LOCUS       DAZGHF010000001       110985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGHF010000001 DAZGHF010000000
VERSION     DAZGHF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 110985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 110985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..110985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.001"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            189..449
                     /gene="iraP"
                     /locus_tag="ACHK3A_000001"
     CDS             189..449
                     /gene="iraP"
                     /locus_tag="ACHK3A_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO8738160.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            549..1964
                     /gene="phoA"
                     /locus_tag="ACHK3A_000002"
     CDS             549..1964
                     /gene="phoA"
                     /locus_tag="ACHK3A_000002"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO8738161.1"
                     /translation="MKQSTIALALLPLLFSPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATVGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            2083..2403
                     /gene="psiF"
                     /locus_tag="ACHK3A_000003"
     CDS             2083..2403
                     /gene="psiF"
                     /locus_tag="ACHK3A_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO8738162.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            2505..3620
                     /gene="adrA"
                     /locus_tag="ACHK3A_000004"
                     /gene_synonym="dgcC"
     CDS             2505..3620
                     /gene="adrA"
                     /locus_tag="ACHK3A_000004"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO8738163.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3637..4446)
                     /gene="proC"
                     /locus_tag="ACHK3A_000005"
     CDS             complement(3637..4446)
                     /gene="proC"
                     /locus_tag="ACHK3A_000005"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO8738164.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            4566..5024
                     /gene="yaiI"
                     /locus_tag="ACHK3A_000006"
     CDS             4566..5024
                     /gene="yaiI"
                     /locus_tag="ACHK3A_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO8738165.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5207..5731
                     /gene="aroL"
                     /locus_tag="ACHK3A_000007"
     CDS             5207..5731
                     /gene="aroL"
                     /locus_tag="ACHK3A_000007"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO8738166.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            5781..5972
                     /gene="yaiA"
                     /locus_tag="ACHK3A_000008"
     CDS             5781..5972
                     /gene="yaiA"
                     /locus_tag="ACHK3A_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO8738167.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            6230..6907
                     /gene="aroM"
                     /locus_tag="ACHK3A_000009"
     CDS             6230..6907
                     /gene="aroM"
                     /locus_tag="ACHK3A_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO8738168.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            6979..7263
                     /gene="ppnP"
                     /locus_tag="ACHK3A_000010"
     CDS             6979..7263
                     /gene="ppnP"
                     /locus_tag="ACHK3A_000010"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO8738169.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSLFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            7420..7749
                     /locus_tag="ACHK3A_000011"
                     /pseudo
     CDS             7420..7749
                     /locus_tag="ACHK3A_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            7746..7826
                     /locus_tag="ACHK3A_000012"
     CDS             7746..7826
                     /locus_tag="ACHK3A_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738170.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(7907..8818)
                     /gene="rdgC"
                     /locus_tag="ACHK3A_000013"
     CDS             complement(7907..8818)
                     /gene="rdgC"
                     /locus_tag="ACHK3A_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO8738171.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            8943..9851
                     /gene="mak"
                     /locus_tag="ACHK3A_000014"
     CDS             8943..9851
                     /gene="mak"
                     /locus_tag="ACHK3A_000014"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO8738172.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(9990..11174)
                     /gene="araJ"
                     /locus_tag="ACHK3A_000015"
     CDS             complement(9990..11174)
                     /gene="araJ"
                     /locus_tag="ACHK3A_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO8738173.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(11300..14443)
                     /gene="sbcC"
                     /locus_tag="ACHK3A_000016"
     CDS             complement(11300..14443)
                     /gene="sbcC"
                     /locus_tag="ACHK3A_000016"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO8738174.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISSMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIKEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSVREHLRQWQQQLTHA
                     EQKLNALTAITLTLTADEVATALAQHAEQRTLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTMEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            complement(14440..15642)
                     /gene="sbcD"
                     /locus_tag="ACHK3A_000017"
     CDS             complement(14440..15642)
                     /gene="sbcD"
                     /locus_tag="ACHK3A_000017"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO8738175.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            15832..16521
                     /gene="phoB"
                     /locus_tag="ACHK3A_000018"
     CDS             15832..16521
                     /gene="phoB"
                     /locus_tag="ACHK3A_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO8738176.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            16579..17874
                     /gene="phoR"
                     /locus_tag="ACHK3A_000019"
     CDS             16579..17874
                     /gene="phoR"
                     /locus_tag="ACHK3A_000019"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO8738177.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKVTRFSFVIPERLIAKNSD"
     gene            18281..19600
                     /gene="brnQ"
                     /locus_tag="ACHK3A_000020"
     CDS             18281..19600
                     /gene="brnQ"
                     /locus_tag="ACHK3A_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO8738178.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            19676..21049
                     /gene="proY"
                     /locus_tag="ACHK3A_000021"
     CDS             19676..21049
                     /gene="proY"
                     /locus_tag="ACHK3A_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO8738179.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     SLKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENH"
     gene            21205..23022
                     /gene="malZ"
                     /locus_tag="ACHK3A_000022"
     CDS             21205..23022
                     /gene="malZ"
                     /locus_tag="ACHK3A_000022"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO8738180.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVPMHKQRSQPQPGVTAWRAAIDLSGGLPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLYQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23027..23608)
                     /gene="acpH"
                     /locus_tag="ACHK3A_000023"
     CDS             complement(23027..23608)
                     /gene="acpH"
                     /locus_tag="ACHK3A_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO8738181.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     NSWYDLDAHYDALETCFWQFYPRMMEQASRKAL"
     gene            23701..24771
                     /gene="queA"
                     /locus_tag="ACHK3A_000024"
     CDS             23701..24771
                     /gene="queA"
                     /locus_tag="ACHK3A_000024"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO8738182.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDERINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24826..25953
                     /gene="tgt"
                     /locus_tag="ACHK3A_000025"
     CDS             24826..25953
                     /gene="tgt"
                     /locus_tag="ACHK3A_000025"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO8738183.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            25976..26308
                     /gene="yajC"
                     /locus_tag="ACHK3A_000026"
     CDS             25976..26308
                     /gene="yajC"
                     /locus_tag="ACHK3A_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO8738184.1"
                     /translation="MSFFISDAVAATGASAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            26336..28183
                     /gene="secD"
                     /locus_tag="ACHK3A_000027"
     CDS             26336..28183
                     /gene="secD"
                     /locus_tag="ACHK3A_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO8738185.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            28194..29165
                     /gene="secF"
                     /locus_tag="ACHK3A_000028"
     CDS             28194..29165
                     /gene="secF"
                     /locus_tag="ACHK3A_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO8738186.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            29295..29642
                     /gene="yajD"
                     /locus_tag="ACHK3A_000029"
     CDS             29295..29642
                     /gene="yajD"
                     /locus_tag="ACHK3A_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO8738187.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(29680..30564)
                     /gene="tsx"
                     /locus_tag="ACHK3A_000030"
     CDS             complement(29680..30564)
                     /gene="tsx"
                     /locus_tag="ACHK3A_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO8738188.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(30863..31402)
                     /gene="yajI"
                     /locus_tag="ACHK3A_000031"
     CDS             complement(30863..31402)
                     /gene="yajI"
                     /locus_tag="ACHK3A_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO8738189.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            31553..32002
                     /gene="nrdR"
                     /locus_tag="ACHK3A_000032"
     CDS             31553..32002
                     /gene="nrdR"
                     /locus_tag="ACHK3A_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO8738190.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            32006..33109
                     /gene="ribD"
                     /locus_tag="ACHK3A_000033"
     CDS             32006..33109
                     /gene="ribD"
                     /locus_tag="ACHK3A_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO8738191.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            33198..33668
                     /gene="ribE"
                     /locus_tag="ACHK3A_000034"
     CDS             33198..33668
                     /gene="ribE"
                     /locus_tag="ACHK3A_000034"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO8738192.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            33688..34107
                     /gene="nusB"
                     /locus_tag="ACHK3A_000035"
     CDS             33688..34107
                     /gene="nusB"
                     /locus_tag="ACHK3A_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO8738193.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            34185..35162
                     /gene="thiL"
                     /locus_tag="ACHK3A_000036"
     CDS             34185..35162
                     /gene="thiL"
                     /locus_tag="ACHK3A_000036"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO8738194.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            35140..35658
                     /gene="pgpA"
                     /locus_tag="ACHK3A_000037"
     CDS             35140..35658
                     /gene="pgpA"
                     /locus_tag="ACHK3A_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO8738195.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDHNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(35712..36686)
                     /gene="yajO"
                     /locus_tag="ACHK3A_000038"
     CDS             complement(35712..36686)
                     /gene="yajO"
                     /locus_tag="ACHK3A_000038"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO8738196.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESNENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(36741..38603)
                     /gene="dxs"
                     /locus_tag="ACHK3A_000039"
     CDS             complement(36741..38603)
                     /gene="dxs"
                     /locus_tag="ACHK3A_000039"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO8738197.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEIVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            complement(38628..39527)
                     /gene="ispA"
                     /locus_tag="ACHK3A_000040"
     CDS             complement(38628..39527)
                     /gene="ispA"
                     /locus_tag="ACHK3A_000040"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO8738198.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLEALERIHRHKTGALIRAAVRLGALSAGDKGRRALSVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(39527..39769)
                     /gene="xseB"
                     /locus_tag="ACHK3A_000041"
     CDS             complement(39527..39769)
                     /gene="xseB"
                     /locus_tag="ACHK3A_000041"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO8738199.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            39975..41423
                     /gene="thiI"
                     /locus_tag="ACHK3A_000042"
     CDS             39975..41423
                     /gene="thiI"
                     /locus_tag="ACHK3A_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HFO8738200.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(41477..42067)
                     /gene="yajL"
                     /locus_tag="ACHK3A_000043"
     CDS             complement(41477..42067)
                     /gene="yajL"
                     /locus_tag="ACHK3A_000043"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO8738201.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(42030..42941)
                     /gene="panE"
                     /locus_tag="ACHK3A_000044"
     CDS             complement(42030..42941)
                     /gene="panE"
                     /locus_tag="ACHK3A_000044"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO8738202.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGITVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            43109..43600
                     /gene="yajQ"
                     /locus_tag="ACHK3A_000045"
     CDS             43109..43600
                     /gene="yajQ"
                     /locus_tag="ACHK3A_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO8738203.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(43728..45092)
                     /gene="yajR"
                     /locus_tag="ACHK3A_000046"
     CDS             complement(43728..45092)
                     /gene="yajR"
                     /locus_tag="ACHK3A_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8738204.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILAATGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADTAANEALEVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(45241..46131)
                     /gene="cyoE"
                     /locus_tag="ACHK3A_000047"
     CDS             complement(45241..46131)
                     /gene="cyoE"
                     /locus_tag="ACHK3A_000047"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO8738205.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(46143..46472)
                     /gene="cyoD"
                     /locus_tag="ACHK3A_000048"
     CDS             complement(46143..46472)
                     /gene="cyoD"
                     /locus_tag="ACHK3A_000048"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO8738206.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(46472..47086)
                     /gene="cyoC"
                     /locus_tag="ACHK3A_000049"
     CDS             complement(46472..47086)
                     /gene="cyoC"
                     /locus_tag="ACHK3A_000049"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO8738207.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKVFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVVSWL
                     ALTWLFGAGFIGMELYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(47076..49067)
                     /gene="cyoB"
                     /locus_tag="ACHK3A_000050"
     CDS             complement(47076..49067)
                     /gene="cyoB"
                     /locus_tag="ACHK3A_000050"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO8738208.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(49089..50036)
                     /gene="cyoA"
                     /locus_tag="ACHK3A_000051"
     CDS             complement(49089..50036)
                     /gene="cyoA"
                     /locus_tag="ACHK3A_000051"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO8738209.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(50496..51971)
                     /gene="ampG"
                     /locus_tag="ACHK3A_000052"
     CDS             complement(50496..51971)
                     /gene="ampG"
                     /locus_tag="ACHK3A_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO8738210.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(52015..52593)
                     /gene="yajG"
                     /locus_tag="ACHK3A_000053"
     CDS             complement(52015..52593)
                     /gene="yajG"
                     /locus_tag="ACHK3A_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8738211.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            52898..53215
                     /gene="bolA"
                     /locus_tag="ACHK3A_000054"
     CDS             52898..53215
                     /gene="bolA"
                     /locus_tag="ACHK3A_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO8738212.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            53559..54857
                     /gene="tig"
                     /locus_tag="ACHK3A_000055"
     CDS             53559..54857
                     /gene="tig"
                     /locus_tag="ACHK3A_000055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO8738213.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            55103..55726
                     /gene="clpP"
                     /locus_tag="ACHK3A_000056"
     CDS             55103..55726
                     /gene="clpP"
                     /locus_tag="ACHK3A_000056"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO8738214.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            55852..57126
                     /gene="clpX"
                     /locus_tag="ACHK3A_000057"
     CDS             55852..57126
                     /gene="clpX"
                     /locus_tag="ACHK3A_000057"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO8738215.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            57314..59668
                     /gene="lon"
                     /locus_tag="ACHK3A_000058"
     CDS             57314..59668
                     /gene="lon"
                     /locus_tag="ACHK3A_000058"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO8738216.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            59859..60149
                     /gene="hupB"
                     /locus_tag="ACHK3A_000059"
     CDS             59859..60149
                     /gene="hupB"
                     /locus_tag="ACHK3A_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO8738217.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            60341..62212
                     /gene="ppiD"
                     /locus_tag="ACHK3A_000060"
     CDS             60341..62212
                     /gene="ppiD"
                     /locus_tag="ACHK3A_000060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO8738218.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVVQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKK
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            62363..62734
                     /gene="ybaV"
                     /locus_tag="ACHK3A_000061"
     CDS             62363..62734
                     /gene="ybaV"
                     /locus_tag="ACHK3A_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO8738219.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            62828..63226
                     /gene="fadM"
                     /locus_tag="ACHK3A_000062"
     CDS             62828..63226
                     /gene="fadM"
                     /locus_tag="ACHK3A_000062"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO8738220.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(63278..63973)
                     /gene="queC"
                     /locus_tag="ACHK3A_000063"
     CDS             complement(63278..63973)
                     /gene="queC"
                     /locus_tag="ACHK3A_000063"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO8738221.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLHHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(64038..65738)
                     /gene="ybaE"
                     /locus_tag="ACHK3A_000064"
     CDS             complement(64038..65738)
                     /gene="ybaE"
                     /locus_tag="ACHK3A_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO8738222.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLLLLQLPARDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            65838..66656
                     /gene="cof"
                     /locus_tag="ACHK3A_000065"
     CDS             65838..66656
                     /gene="cof"
                     /locus_tag="ACHK3A_000065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO8738223.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGNVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            66809..67267
                     /gene="decR"
                     /locus_tag="ACHK3A_000066"
     CDS             66809..67267
                     /gene="decR"
                     /locus_tag="ACHK3A_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO8738224.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            67297..69069
                     /gene="mdlA"
                     /locus_tag="ACHK3A_000067"
     CDS             67297..69069
                     /gene="mdlA"
                     /locus_tag="ACHK3A_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO8738225.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAEDAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            69062..70843
                     /gene="mdlB"
                     /locus_tag="ACHK3A_000068"
     CDS             69062..70843
                     /gene="mdlB"
                     /locus_tag="ACHK3A_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO8738226.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVIMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDANTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            71024..71362
                     /gene="glnK"
                     /locus_tag="ACHK3A_000069"
     CDS             71024..71362
                     /gene="glnK"
                     /locus_tag="ACHK3A_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO8738227.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            71392..72678
                     /gene="amtB"
                     /locus_tag="ACHK3A_000070"
     CDS             71392..72678
                     /gene="amtB"
                     /locus_tag="ACHK3A_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO8738228.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(72727..73587)
                     /gene="tesB"
                     /locus_tag="ACHK3A_000071"
     CDS             complement(72727..73587)
                     /gene="tesB"
                     /locus_tag="ACHK3A_000071"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO8738229.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            73805..74374
                     /gene="ybaY"
                     /locus_tag="ACHK3A_000072"
     CDS             73805..74374
                     /gene="ybaY"
                     /locus_tag="ACHK3A_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO8738230.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQPAI
                     QQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFVL
                     PFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPVQ
                     ASGGATATVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(74405..74716)
                     /locus_tag="ACHK3A_000073"
     CDS             complement(74405..74716)
                     /locus_tag="ACHK3A_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO8738231.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            74879..74975
                     /gene="ffs"
                     /locus_tag="ACHK3A_000074"
     ncRNA           74879..74975
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHK3A_000074"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            75095..75448
                     /gene="ybaA"
                     /locus_tag="ACHK3A_000075"
     CDS             75095..75448
                     /gene="ybaA"
                     /locus_tag="ACHK3A_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO8738232.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(75490..77040)
                     /gene="pdeB"
                     /locus_tag="ACHK3A_000076"
     CDS             complement(75490..77040)
                     /gene="pdeB"
                     /locus_tag="ACHK3A_000076"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO8738233.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWSSTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(77204..77674)
                     /gene="ylaC"
                     /locus_tag="ACHK3A_000077"
     CDS             complement(77204..77674)
                     /gene="ylaC"
                     /locus_tag="ACHK3A_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO8738234.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(77790..78341)
                     /gene="maa"
                     /locus_tag="ACHK3A_000078"
     CDS             complement(77790..78341)
                     /gene="maa"
                     /locus_tag="ACHK3A_000078"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO8738235.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(78514..78732)
                     /gene="hha"
                     /locus_tag="ACHK3A_000079"
     CDS             complement(78514..78732)
                     /gene="hha"
                     /locus_tag="ACHK3A_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO8738236.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(78758..79132)
                     /gene="tomB"
                     /locus_tag="ACHK3A_000080"
     CDS             complement(78758..79132)
                     /gene="tomB"
                     /locus_tag="ACHK3A_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO8738237.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(79678..82827)
                     /gene="acrB"
                     /locus_tag="ACHK3A_000081"
     CDS             complement(79678..82827)
                     /gene="acrB"
                     /locus_tag="ACHK3A_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO8738238.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(82850..84043)
                     /gene="acrA"
                     /locus_tag="ACHK3A_000082"
     CDS             complement(82850..84043)
                     /gene="acrA"
                     /locus_tag="ACHK3A_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO8738239.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATTLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            84185..84832
                     /gene="acrR"
                     /locus_tag="ACHK3A_000083"
     CDS             84185..84832
                     /gene="acrR"
                     /locus_tag="ACHK3A_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO8738240.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            84960..88322
                     /gene="mscK"
                     /locus_tag="ACHK3A_000084"
     CDS             84960..88322
                     /gene="mscK"
                     /locus_tag="ACHK3A_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO8738241.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLRDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            88383..88481
                     /locus_tag="ACHK3A_000085"
                     /pseudo
     CDS             88383..88481
                     /locus_tag="ACHK3A_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(88534..88695)
                     /gene="rsmS"
                     /locus_tag="ACHK3A_000086"
     CDS             complement(88534..88695)
                     /gene="rsmS"
                     /locus_tag="ACHK3A_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO8738242.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(88709..89236)
                     /gene="priC"
                     /locus_tag="ACHK3A_000087"
     CDS             complement(88709..89236)
                     /gene="priC"
                     /locus_tag="ACHK3A_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO8738243.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            89306..89683
                     /gene="ybaN"
                     /locus_tag="ACHK3A_000088"
     CDS             89306..89683
                     /gene="ybaN"
                     /locus_tag="ACHK3A_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO8738244.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            89836..90387
                     /gene="apt"
                     /locus_tag="ACHK3A_000089"
     CDS             89836..90387
                     /gene="apt"
                     /locus_tag="ACHK3A_000089"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO8738245.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            90516..92447
                     /gene="dnaX"
                     /locus_tag="ACHK3A_000090"
     CDS             90516..92447
                     /gene="dnaX"
                     /locus_tag="ACHK3A_000090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO8738246.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSSAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNSVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    91780..91844
                     /gene="dnaX"
                     /locus_tag="ACHK3A_000090"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            92500..92829
                     /gene="ybaB"
                     /locus_tag="ACHK3A_000091"
     CDS             92500..92829
                     /gene="ybaB"
                     /locus_tag="ACHK3A_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO8738247.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            92829..93434
                     /gene="recR"
                     /locus_tag="ACHK3A_000092"
     CDS             92829..93434
                     /gene="recR"
                     /locus_tag="ACHK3A_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO8738248.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            93544..95418
                     /gene="htpG"
                     /locus_tag="ACHK3A_000093"
     CDS             93544..95418
                     /gene="htpG"
                     /locus_tag="ACHK3A_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO8738249.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            95599..96243
                     /gene="adk"
                     /locus_tag="ACHK3A_000094"
     CDS             95599..96243
                     /gene="adk"
                     /locus_tag="ACHK3A_000094"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO8738250.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            96375..97337
                     /gene="hemH"
                     /locus_tag="ACHK3A_000095"
     CDS             96375..97337
                     /gene="hemH"
                     /locus_tag="ACHK3A_000095"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO8738251.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(97334..98293)
                     /gene="aes"
                     /locus_tag="ACHK3A_000096"
     CDS             complement(97334..98293)
                     /gene="aes"
                     /locus_tag="ACHK3A_000096"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO8738252.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FTGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            98445..99749
                     /gene="gsk"
                     /locus_tag="ACHK3A_000097"
     CDS             98445..99749
                     /gene="gsk"
                     /locus_tag="ACHK3A_000097"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO8738253.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(99833..101509)
                     /gene="ybaL"
                     /locus_tag="ACHK3A_000098"
     CDS             complement(99833..101509)
                     /gene="ybaL"
                     /locus_tag="ACHK3A_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO8738254.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(101747..102967)
                     /gene="fsr"
                     /locus_tag="ACHK3A_000099"
     CDS             complement(101747..102967)
                     /gene="fsr"
                     /locus_tag="ACHK3A_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO8738255.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            103185..104837
                     /gene="ushA"
                     /locus_tag="ACHK3A_000100"
     CDS             103185..104837
                     /gene="ushA"
                     /locus_tag="ACHK3A_000100"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO8738256.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLIMAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNNLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKAGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(104874..105353)
                     /gene="ybaK"
                     /locus_tag="ACHK3A_000101"
     CDS             complement(104874..105353)
                     /gene="ybaK"
                     /locus_tag="ACHK3A_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO8738257.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(105557..106351)
                     /gene="ybaP"
                     /locus_tag="ACHK3A_000102"
     CDS             complement(105557..106351)
                     /gene="ybaP"
                     /locus_tag="ACHK3A_000102"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO8738258.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEKRISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            106484..106786
                     /locus_tag="ACHK3A_000103"
     CDS             106484..106786
                     /locus_tag="ACHK3A_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8738259.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            106822..107163
                     /gene="ybaQ"
                     /locus_tag="ACHK3A_000104"
     CDS             106822..107163
                     /gene="ybaQ"
                     /locus_tag="ACHK3A_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO8738260.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(107221..109725)
                     /gene="copA"
                     /locus_tag="ACHK3A_000105"
     CDS             complement(107221..109725)
                     /gene="copA"
                     /locus_tag="ACHK3A_000105"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO8738261.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTNEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            109988..110920
                     /gene="glsA"
                     /locus_tag="ACHK3A_000106"
     CDS             109988..110920
                     /gene="glsA"
                     /locus_tag="ACHK3A_000106"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO8738262.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT        27049 a        27993 c        29523 g        26420 t
ORIGIN      
        1 aggttgataa ttaaaagcct atattttgtg tgggtaatta ttaaaataag agaaacgttt
       61 cgctggtaat caaacaaaaa atatttgcgc aaagtatttc ctttgtcata aaaataatac
      121 ttccagacac tatgaagttg tgaaacataa tgttaacttc tccatacttt ggataaggaa
      181 atacagacat gaaaaatctc attgctgagt tgttatttaa gcttgcccaa aaagaagaag
      241 agtcgaaaga actgtgcgcg caggtagaag ctttggagat tatcgtcact gcaatgcttc
      301 gcaatatggc gcaaaatgac caacagaggt tgattgatca ggtagagggg gcgctgtacg
      361 aggtaaagcc cgatgccagc attcctgacg acgatacgga gctgctgcgc aattatgtaa
      421 agaagttatt gaagcatcct cgtcagtaaa aagttaatct tttcaacagc tgtcataaag
      481 ctgtcacggc cgagacttat agtcgctttg tttttatttt taatgtattt gtacatggag
      541 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg ttttcccctg
      601 tgacaaaagc ccggacacca gaaatgcctg ttctggaaaa ccgggctgct cagggcgata
      661 ttactgcacc cggcggtgct cgccgtttaa cgggggatca gaccgccgct ctgcgtgatt
      721 ctcttagcga taaacccgcc aaaaatatta ttttgctgat tggcgatggg atgggggact
      781 cggaaattac tgctgcacgc aattatgccg aaggtgcggg cggctttttt aaaggtatcg
      841 atgcattacc gcttaccggg caatacactc actatgcgct gaataaaaaa accggtaaac
      901 cggactacgt caccgactcg gctgcatcag caaccgcctg gtcaactggt gtcaaaacct
      961 ataacggtgc gctgggcgtc gatattcacg aaaaagatca cccaacgatt ctggaaatgg
     1021 caaaagccgc aggtctggcg accggtaacg tttctaccgc agagttgcag gatgccacgc
     1081 ccgccgcgct ggtggcgcat gtgacctcgc gcaaatgcta cggtccgagc gcgaccagtg
     1141 aaaaatgtcc gggtaacgct ctggaaaaag gcggaaaagg atcgattacc gaacagctgc
     1201 ttaacgctcg tgccgatgtt acgcttggcg gcggcgcaaa aacctttgct gaaacggcaa
     1261 ccgtcggcga atggcaggga aaaacgctgc gtgaacaggc acaggcgcgt ggttatcagt
     1321 tggtgagtga tgctgcctcg ctaaatgcgg tgacggaagc gaatcagcaa aaacccctgc
     1381 ttggcctgtt tgctgacggc aatatgccag tgcgctggct aggaccgaaa gcaacgtacc
     1441 acggcaatat cgataagccc gcagttacct gtacgcctaa tccgcaacgt aatgacagtg
     1501 taccgaccct ggcgcagatg accgacaaag ccattgaatt gttgagtaaa aatgagaaag
     1561 gttttttcct gcaagttgaa ggtgcatcca tcgataaaca ggatcacgct gcgaatcctt
     1621 gtgggcaaat tggcgagacg gtcgatctcg atgaagccgt acaacgtgcg ctggaattcg
     1681 ctaaaaagga tggcaacacg ctggtcatag tcaccgctga tcacgcccac gccagccaga
     1741 ttgtcgcgcc ggacaccaaa gcgccgggcc tcacccaggc gctaaatacc aaagatggcg
     1801 cagtgatggt gatgagttac gggaactccg aagaggattc acaagaacat accggcagtc
     1861 agttgcgtat tgcagcgtat ggcccacatg ccgccaatgt cgttggactg accgaccaga
     1921 ccgatctctt ctacaccatg aaagccgccc tggggctgaa ataaaaccgc gcccggcggt
     1981 gaattttcgc tgtcgggtgg tttttttgct gttagcaacc agacttaatg gcagatcacg
     2041 ggcgcaaacg ctcatggtta aaacatgaag agggatggtg cgatgaaaat aacattactg
     2101 gttaccttgc ttttcggact ggttttttta accaccgtcg gcgctgccga gagaacctta
     2161 accccgcaac aacagcgtat gacctcctgt aatcagcagg cgacggcgca ggcgttgaaa
     2221 ggggatgctc gtaagaccta catgagtgat tgcctgaaga acagcaagtc tgcgcctggt
     2281 gaaaaaagtt tgacgccaca gcagcaaaag atgcgcgaat gtaataatca agcaacgcaa
     2341 caatctctga aaggggatga tcgtaataag tttatgagcg cctgcctcaa gaaagccgcc
     2401 tgatacctga tagtgctaac gggtgagcta cgaaaatggc tcacccgaaa tttcatactt
     2461 ctgcctttag ccccgtctct ataatttggg aaaattgttt ctgaatgttc ccaaaaataa
     2521 tgaatgatga aaactttttc aaaaaagcgg cggcgcatgg ggaggaacct cctttaactc
     2581 ctcaaaacga acatcagcgg tccgggctgc gctttgcccg tcgcgtcaga ctaccccgcg
     2641 cggttggcct ggctggcatg ttcttaccga ttgcttcaac gctggtttcg catccgtcgc
     2701 cgggctggtg gtggctgttg ttggtcggct gggcgttcgt ctggccgcat ttagcctggc
     2761 aaatagcgag cagggccgtc gatccgctta gccgggaaat ttacaactta aaaaccgatg
     2821 cagtattagc gggaatgtgg gtaggcgtaa tgggcgtaaa cgcactgcct tccaccgcga
     2881 tgttgatgat tatgtgtctg aatttgatgg gggcaggcgg cccccgtctg tttgtcgcgg
     2941 gtctggtgtt gatggtggtc tcctgccttg tcaccctcga gctgacgggc atcaccgtgt
     3001 cgttcaatag cgcaccgctg gaatggtggc tctcccttcc cattattgtc atttatcctc
     3061 tgctgtttgg ctgggtcagc taccagacgg cgactaaact ggcggaacat aaacgcaggt
     3121 tgcaggtcat gagtacccgc gacggtatga cgggcgtgta taaccgacgt cattgggaaa
     3181 ctatgttacg caatgaattt gataactgtc ggcggcataa tcgcgatgca acgttactga
     3241 ttatcgatat cgaccatttc aagagcatca acgatacctg gggccatgat gtgggtgatg
     3301 aagcgattgt ggcgcttacc cgacagttac aaattaccct acgcggtagc gatgtgattg
     3361 gtcggtttgg cggcgatgag tttgcagtaa tcatgtccgg tacgccagca gagagcgcca
     3421 tcaccgccat gttacgggtg catgaagggc taaacacgtt acgcttgccg aatacgccac
     3481 aggtaacttt acggattagt gtgggggttg cgccgctgaa tccacaaatg agtcactatc
     3541 gcgagtggtt gaaatcggca gatttggcgc tctacaaagc aaagaaagcc ggacgtaacc
     3601 gcaccgaagt ggcggcctga cgtccggcaa aagccatcag gatttgctga gtttttctga
     3661 tttttccata cacttcgtca tcgcttcgat cactgcggca cggaagcctt tctcttccag
     3721 tacgcgtacc gcctcaatgg tggtgcctcc cggtgagcag accatatctt tcagtgttcc
     3781 cggatgttct cccgtttcca gcaccatttt tgcggaaccc atgaccgcct gagcggcaaa
     3841 tttatacgcc tgggcgcgtg gcatcccgcc cagcacggcg gcgtcggcca tcgcttcgat
     3901 aaacataaag acgtaggctg gcgaagaacc gctcacaccg accaccgggt ggatcatcgg
     3961 ctcagcaatc acttccgctt cgccaaagca gcggaaaata ttcagcacat cagcggtatc
     4021 ttctggggtt accagcgcgt ttggcgttac ggaggtcatc ccggcattaa ccagcgcggg
     4081 agtgttcggc atggcgcgga taattttccg gtcatggccc agcgcgcggg caagctggtc
     4141 gagcgtgata cctgcagcaa tagaaacgac cagagagtct ttattcaggc tggaggtgat
     4201 ttcgctaagc actttaatca tgatgcctgg tttaacggcg gcaaaaatga tgtcggcgat
     4261 ttgcgctact tcttgcgccg attctgcggc gttgatgccg aactggtcat gcagggcggt
     4321 gactttatcc ggggaggggg tatataccca gatttgccct ggaagcacct gaccgctggc
     4381 aatcagaccg ccgagaatgg cttttcccat attgccgcag ccaataaaac cgattttctt
     4441 ttccattgcc tcactcctgc cgtgaaattc attgttttga taatcgctgg cagaagcata
     4501 aacagaacta tgccggaagg caaaagcgcg acacaataga ggattaccca acaaaggatg
     4561 actttatgac aatttgggtg gatgccgacg cgtgtcccaa tgtaattaaa gagattttgt
     4621 atcgcgcggc ggaacgtatg cagatgccgc tggtactggt agcaaaccag agtttacgcg
     4681 tgccgccatc gcgatttatt cgtacgctgc gcgtcgcggc aggtttcgac gttgctgata
     4741 acgaaattgt tcggcagtgt gaagcgggcg atttggtgat caccgcagat atacctttgg
     4801 ctgctgaagc catcgagaaa ggcgcggcgg cgcttaatcc gcgcggcgaa cgttacacgc
     4861 cagcgaccat tcgtgagcgc ctgacgatgc gcgattttat ggatacctta cgtgccagtg
     4921 ggatccagac cggaggacca gatagtcttt cacaacgtga ccgccaggct tttgctgcgg
     4981 agctggagaa gtggtggctg gaagtgcaac gtagtcgtgg ctaaatgtaa tttattattt
     5041 acacttcatt cttgaatatt tattggtata gtaagggatg tattgagatt ttcactttaa
     5101 gtggaatttt ttctttacaa tcgaaattgt actagtttga tggtatgatc gctattctca
     5161 tgacaccggc tttcgccgca ttgcgaccta ttggggaaaa cccacgatga cacaacctct
     5221 ttttctgatc gggcctcggg gctgtggtaa aacaacggtc ggaatggccc ttgccgattc
     5281 gcttaaccgt cggtttgtcg ataccgacca gtggttgcaa tcacagctca atatgacggt
     5341 cgcggagatc gtcgaaaggg aagagtgggc gggattccgc gccagagaaa cggcggcgct
     5401 ggaagcggta actgcgccat ccaccgttat cgctacaggc ggcggcatta ttctgacgga
     5461 atttaatcgt cacttcatgc aaaataacgg gatcgtggtt tatttgtgtg cgccagtatc
     5521 agtcctggtt aaccgactgc aagctgcacc ggaagaagat ttacggccaa ccttaacggg
     5581 aaaaccactg agcgaagaag ttcaggaagt gctggaagaa cgcgatgcgc tatatcgcga
     5641 agttgcgcat attatcatcg acgcaacaaa cgaacccagc caggtgattt ctgaaattcg
     5701 cagcgctctg gcacagacga tcaattgttg attttcgagc gcctatactt aacgttcatc
     5761 ccgtgaaata aggaagaacg atgccaacga aaccgcctta tcctcgtgaa gcatatatag
     5821 tgacgattga aaaaggaaag ccaggacaga cggtaacctg gtaccaactc agagccgatc
     5881 atcctaaacc agactcgttg atcagtgaac atccgaccgc tcaggaagcg atggatgcga
     5941 aaaaacgcta tgaggaccct gacaaagagt gaccgcatca gactgctcgg aagggattct
     6001 gagtgccgcc acaagggatc tgcgtcacat ttttcacaat tcatgttttt caaataatta
     6061 gaatattaaa caataacaat ccattactgg aatcatttgg aatctttaca ttatgcagtg
     6121 caggtctgct gctacgcttt ttgtcatttg tagcacaagt aagtgtcagc agtggtgctt
     6181 aacacttgcc cggtaattaa cgacgaaata aaagtaaggt ggaagaacaa tgagtgcgtc
     6241 gttggcgatc ctgaccatcg gcattgtacc tatgcaggaa gttttgccgc tcctgacaga
     6301 atacattgac gaagataata tttcccatca tagcctgctg gggaagttaa gccgcgaaga
     6361 agtgatggcg gagtacgcgc cagaagcagg cgaagacacc attctcacat tattaaatga
     6421 caaccatctg gcccatgttt cgcgtcgcaa agtggagcga gacctgcaag gtgtggttga
     6481 agtgctcgat aatcagggtt acgacgtcat tatattaatg agtacagcaa acattagtag
     6541 tatgaccgcg cgtaatacga tctttcttga gccgtcgcgg atattgccac cgctggtttc
     6601 ctctattgtt gaagatcatc aggtgggggt tatcgtaccg gttgaggagt tgctgaccgt
     6661 tcaggcgcaa aaatggcaaa ttttgcagaa accgccagta ttttcattgg gtaatcccat
     6721 tcatgattcg gaacaaaaaa tcattgatgc cgggaaagaa ttactggcaa aaggtgcaga
     6781 tgtcatcatg ctggattgtt tgggatttaa ccagcgtcat cgcgatttac tgcaaaaaca
     6841 gctcgatgtt cctgtcttgc tctctaacgt attgattgca cggctggctg cggaattact
     6901 gatgtaattt tgcgtgacag ccagcgcctc tggcccctat agtgaagtag atgttcaact
     6961 accagacagg gccagtttat gcttcaaagt aatgagtact tttccggcaa agtgaaatcg
     7021 atcggctttt ccagcagcag cactggtcgc gccagcgtgg gtgttatggt tgaaggtgaa
     7081 tacaccttca gcaccgctga gcctgaagag atgacggtaa tcagtggcgc gctgaatgtg
     7141 ttactgcctg acgcgaccga ctggcaagtg tatgaagccg gttcgctgtt taatgttccc
     7201 ggtcacagcg agtttcatct gcaagttgcc gaacccacct cttatctgtg ccgctatctg
     7261 taattcctcg ccttcccctt gaacgggagg gcatttttct gaaatgtcct ctctttaacc
     7321 cataataata tttcctttgc tgggattttt tcgatttccg atatattcat aattcatcaa
     7381 ggttgatata aatatcagcg aagatctcca gatactattg cggaactggc gacgttaaaa
     7441 gataaatcag atgatgaatg gtggtgtgca ttgctgcaaa gtcgcaatga tcatttgcgc
     7501 caaacggcat tgcacaacgc acatactccg gtgttgttaa cgacattgac tgagcctcag
     7561 gagcggtcgc tggctattaa taattcacag ctggctgccg atgtgaaaac ggcgtggtta
     7621 aaagaggagc catcattact cttatttgtc gaacaacccg atctttcgct gttacgtgat
     7681 ttagtgaaaa acggggcaac gcgaaaaatt cgcagtgaag cgcgtcaccg gcttgaggaa
     7741 aaacaatgac acaacgcccc tggagcaagt tacagcataa aacgcataat attgctgcgc
     7801 taagaattat tgcccgctgt agctaacgat tatattcctg gtgtggcttc gtacgccgga
     7861 taagacgcgg caggcgtcgc atccggcatt aaaggtaaat cagcaattaa cgttgtgctt
     7921 cgccacctaa tccttcaatc aggttttgaa ttaacgctgc cagttcacca gtcatcagga
     7981 tgaaatcggc atcaaagcgc tgggcgaaat cttcacggtc gatatcttcg ttttgatcgc
     8041 gcagctcgtc gcagaacttc agacgcttga gcgaaccatc gtcgcacatc acaaactgaa
     8101 tgcgctgctg ccagtcgagc gccagtttag tcaccacttt tccggcttca atgtggttgg
     8161 tgatctcttc gctggtcaga tcttgtttct tcgcgcggat cactccgcca tcttccagca
     8221 acgatttcag ctcggcttca tcaagcagct ggaagccctg tgccgcgctg ccggagcgaa
     8281 cccattcggt cagcgtcagt tcaatcgggt tttccatgct caatggtaca accggtaacg
     8341 accccaggct tttacgcagc agtgccagcg tatcttccgc ttttttggca ctggcgcagt
     8401 ccaccataat caaaccgtta accgtgtcga tccacatcat tgtctggcta aaacggctga
     8461 aagcacgcgg tagcagagag tgcagcactt cgtctttcag cgaatctttt tcggttttct
     8521 tcagcttacg cgcctgttcc gcttccagtt tggcgatttt cgcttccagc gcctgtttaa
     8581 tcaccggaga cgggaggatt ttttcttctt tgcgcgcgca gataacaatt tgaccattgg
     8641 caacgtgcgt taacgcatcg ctgtgcgatc ccatcggagg aacccagccc atcttcgcca
     8701 tgtcctggct gccgcatggg gtaaatgcca tcgaggctag ctgtttttcc atctcttctg
     8761 cacgcaacga aatctcgcgg ctaagacggt aaaccattaa atttttgaac cacagcatga
     8821 taatttccac ggccttgtcg ttaaatttag cgggcatgat aacgaattgt cggcggcctt
     8881 gcattgccaa tccagttgtc cgtctctacg ctattgatat tgaaaaaaat aaggagagta
     8941 ccgtgcgtat aggtatcgat ttaggcggca ccaaaactga agtgattgca ctgggcgatg
     9001 caggggagca gttgtaccgc catcgtctgc ccacgccgcg tgatgattat cggcagacta
     9061 ttgaaacgat cgccacgttg gttgatatgg cggagcaggc aacagggcag cgcggaacgg
     9121 taggtatggg cattcctggt tcgatttcgc cctacaccgg cgtggtgaag aatgccaatt
     9181 caacctggct caacggtcag ccattcgata aagacttaag cgcgaggttg cagcgggaag
     9241 tgcggctggc aaatgacgcc aactgtctgg cggtttcaga agcagtagat ggcgcggcag
     9301 cgggagcgca gacggtattt gccgtgatta tcggcacggg atgcggcgcg ggcgtggcat
     9361 tcaatgggcg ggcgcatatc ggcggcaatg gcacggcagg tgagtgggga cacaatccgc
     9421 taccgtggat ggacgaagac gaactgcgtt atcgcgagga agtcccttgt tattgcggta
     9481 aacaaggttg tattgaaacc tttatttcgg gcacgggatt tgcgacggat tatcgtcgtt
     9541 tgagcggaca tgcgctgaaa ggcagtgaaa ttatccgcct ggttgaagaa agcgatccgg
     9601 tagcggaact ggcattgcgt cgctacgagc tgcggctggc aaaatcgctg gcacatgtcg
     9661 tgaatattct cgatccggat gtgattgtcc tggggggcgg gatgagcaat gtagaccgtt
     9721 tatatcaaac ggttccgcag ttgattaaac aatttgtctt cggcggcgaa tgtgaaacgc
     9781 cggtgcgtaa ggcgaagcac ggtgattcca gcggcgtacg cggcgctgcg tggttatggc
     9841 cacaagagta aaaaaaccag gcaattagcg catcatgcct gatgcgacgc ttgccgcgtc
     9901 ttatcaggcc tacaaaatga gcagcccgta ggccggataa ggcgtttacg ccgcatccgg
     9961 caataagcaa cgcgcacggt gacagtaatc taccccagtg gtttcgccag caccggacta
    10021 tccgccgctt gctggcgctt atagcgacca tacagcagca acgacgacat cgcagcaaac
    10081 gaaagcaggg cggcaggcag cgccacgtaa ttatatgcca gccccagcgt cagcatcata
    10141 ccaccgcaat atgcgccgac ggcgctaccg aggttaaacg ctatttgccc acctgcggca
    10201 cctaataact ctccgccttt ggcattttgc agtaacaata tttgcagtgg tgctgaaagg
    10261 gcaaataatc ccgcgcaaca aataaatgca aaaataagcg acgttgtttt catgccgccg
    10321 aagaaaaaga gcatcagcag tgccagcaca ataataaagt cagttactgc cgcaatgcgc
    10381 agtggtgaat aacgtcctga aatccggcca cttagcacat tccccagcac catccctagc
    10441 ccaactaaca tcataataaa agtcatcgcc gtttccgaaa aaccggaaat aaacatcatg
    10501 tatggcttta cgtagctgaa ccaggcaaat acacctgcgt tgccaaacat ggtggcggcg
    10561 aaaattaacc acggggccgg gctgcgtaaa aagtgaaatt gttcgcgcag ctttcctttc
    10621 gcttcgtcgc gaatatccgg cacccaaaaa tagaccgatg ccatcaccgc aatattaaaa
    10681 acagcgatca ataaaaaggt gtagcgccag ctaaattcct gacttaaata cgttcccagc
    10741 ggaatgccca gcaaattggc gactgtcatc ccggaaacca tccccgccac ggcggcggtg
    10801 acttttccgg gtttgataat ttttgataac acgatcgcgc cgacgccaaa aaatgcgcca
    10861 tgcggaaagc cggataccag ccgaccaatg gcgagcatca ggtaagacga agagagcgtg
    10921 aacatggcgt tgccaatgac gcacaacgcc accagaaaca ataagatatg tttgagtgag
    10981 tagcggctgg aaaagagtgc gatgattggc gcaccgacca ccacccccag cgcataatac
    11041 gagatcatat gcccggcagc aggaatcgaa attcctacgt tatgcgccag ctccgtgagc
    11101 acgcccataa tgccaaattc ggccatcccc aaaccaaacg tgcccagagc caaagataaa
    11161 atgacttttt tcataccacc atcacaacca ccagaacggc taccaatcac cgtagccaat
    11221 aaagtgatag cttgcaggat aacgaccagc aaggccaaga gataattcgc ccctctgtat
    11281 tcattatcct gctgaatagt tatttcactg caaacgcact ttccagtttg ctgtagccca
    11341 ggccgttgat cttcttcact ttgatctgca ccggaatacg ctctttcatc gcttcaacgt
    11401 ggctaatcac accgatggtt ttgccactgg cattcagggc atccagcgca tcaagggcgg
    11461 tatccagcgt ttcgctatcc agcgtgccaa aaccttcatc aaggaacagc gagtcaatac
    11521 gcgttttatg gctgaccaga tccgaaagcg ccagcgccag cgccagactg acgaggaaac
    11581 tttcgccgcc ggaaagggta cgggtatcgc gtaccgcatc tgcctgccag gtatcaacaa
    11641 cctcgacttc cagcgcctcg ctggctttgc gctgtaacag atagcgcccg tgcagccggg
    11701 taagttgctg attagcgaga tggactaaat tatccagcgt cagcccctgg gcaaacttgc
    11761 ggaatttatc gccctctttg gaacctatta gcgaattcag atatccccag tcctcaacct
    11821 gctgcgtcat ttgagcaatt tgctgcatta aggtttgttg ttgctgacgg ttgtctgcat
    11881 cctgcttcag ctgctggcga atctcgccct gactggtggt gttttcacgc aacttttggt
    11941 gagtttgcgc taactcttgc tgaatctgct ccatcgtcac agtgagagcc aacccgtcag
    12001 gtcggtgttg ctgatgctgt gccagcgttt ctgctgtctg agtgaccagc gtttgcgcct
    12061 gacggcgctg gttttccaga ttctgcttga gctgttccag ttgcgttagt gtttgttcat
    12121 ccattagcgc cgcaaggaac gcctgctgat cgtcaaagac gctggcctgt agcgcggtgt
    12181 caaactgcgc ctgggctttt tgcagacttt gcgccgccag aacgtcctgt tgctgtaacg
    12241 tctgctgctg gctgtgtaat gcgagacatt gttcatgaac ctgccgccag ttttccagca
    12301 ccacagtttc ttcgctgtgc gggagttcat cactttgcgg caacgtttcc agaatcggcg
    12361 tcagctgctg catacggttt tgcagcgcgg ttaattcgtt ctggcgatgc tgccagctct
    12421 gcgcttcttg ctgacgcgtc gccaaccagc tctcttcttc atcttcctgt ggcaatgtca
    12481 gtgcataacc cgccaatgcc gttaagagct gctgctggcg ttgttcaatt tgctgttgat
    12541 actggataat ttgctgatta tgcgcggcaa tctgcccttg taattcatgt cgttggctga
    12601 gtaaccgcag ctggcgttcg tgctcatctt gtgcatccag ccacggttga atatcgtcct
    12661 gtggctgcaa ggtgatattg aggctggccg tgacggcttg ccattgttga gtaagtgctt
    12721 gctcatcttg tcggaggctt tgcgcttcgt tttcatcgcg ctgaagttgc tttgttaagg
    12781 cgtccagttg cccacgtagc gtcgcacctt cttcaccgag ctttttaact tcgttttcca
    12841 gcgccagtaa tcgagactga ttaacgccag gctccagcgc ctgatacgcc tcgaccgccg
    12901 ggtggctggt ggaaccacaa agtggacaag gctgacccgc ctgtaactgt gcacgttgag
    12961 cttccagcgt tttgatgcgc gcttcctgct cgcaaatagt tttcacatcg gcaagttgct
    13021 gcgtcttttc tttataacgc tggcgcattt cgttaagtgc ggcgttacgt tgcgtctgtt
    13081 cttgcgtgac attctggata gcgacctgta attgcgccag gcgtttttgt tggggaacaa
    13141 tctgtccatg cagcgcgacc aggcgctgac gcagcgtgcg ttgctcagca tgttgcgcca
    13201 gggcggtagc aacttcatcg gcggttaacg tcaacgtgat cgccgtaagc gcattaagtt
    13261 tttgctcagc atgggttaac tgttgctgcc attgccgcag atgctcgcga acgctggttt
    13321 gttgggagaa ctgcgcacgc caacccgcca gttcgttgtt ccactgacgg aagcggtcgt
    13381 gttcctgtaa ccaggcattc aggctttgtt gctgctgctg taattctgct gactgcttcg
    13441 ccgcgtggtg gcgaatgctc gcccgaagcg ccattgtgct ctgtaagcga gtatttactt
    13501 ctttaatctg ctggcgagta tgcgccagcg ccgcgctgtg ttctgcgatg cgttcccagt
    13561 gcggacgaag atttcgtgcc ggttgtgcca gactgagcgc cgccagttga ggttgcgctt
    13621 tttcttcttc ggctaacgcc tgttgcaagg cctgctgacg gcggctggct tcttgctgca
    13681 attcgtccag acgcgttaac cagtttagtg attgttgttc ttgctgctgc gcggtaagta
    13741 actgtttttc ttcgtcagta agtacctgca aactcgctgt cagcgattgc acttgttccg
    13801 gcgtgagcaa cgcgacgccg ctggcctgcg cttgcagctt ctctagctca gtgcgggccg
    13861 atttgtgctg ctcaaacacc atcgacgaga tttgtccgta gatttcagtg ccggttaact
    13921 cctcgagcaa ttccgcgcgt tctttgggtt tggcattcag gaaggcagca aattgcccct
    13981 gcgaaagcag catcgaacgg gtgaagcgcc cgtaatccag tccggttaac gtcgccgtca
    14041 gttccagctt atctttcact ttgtcggcga gaattttgcc atcggcgcag cgcgccagct
    14101 ctacgcgtgg cacctgcaaa ttaccgtcgg gctggttacg cgcccgattc tggctccaga
    14161 acgcgcgata cgcttcacct ttcacttcaa actccacctc cgccagacat tcggcggtat
    14221 cgcgggtcat gagatcattt tgcgattgtg aaacgttaga aaggcgcgga gtttcgtgat
    14281 acagcgccag acaaatggcg tccagcaggg tggttttccc cgcaccggtt gggccggtaa
    14341 tggcaaacag cccgttgctg gcgaacggct cgcgggtgaa atcaatcttc cattcgcctt
    14401 ttaatgagtt caggtttttc aggcgcaggc tgagaatttt catgcttcgt gttctccggc
    14461 gaggctatgc aacgtcgtgg cgaaaagatg ctgcagacgt tgctgctgcg attcctccag
    14521 ttcttccagt gccagacggc gattgaacac ctcttcgacg ctgagttcgc tgagggtttc
    14581 acgctgttgg ctggctaaca cacgctcgcg ctgttcacga ctccggcgta ccagcaatac
    14641 ttcgacaggc aatgattcgg ttaatgcctg gattttgcgc tgaatatcat gcagatactc
    14701 atcagtagtg atttcgatat ccagccagac aggtggctcc tgtgatacat cgcgccactg
    14761 ttccagctgt tcggtaatcg acgccagatc gcctttcagc accgccatgg gttgcgttac
    14821 cggtacgttc aggttttcca cgctctctaa tttgccgttt gaaaatgtca caagatggac
    14881 atatttactc ttaccgcatt catcaaaact cagtggaatg ggggaaccgc aatatcgaac
    14941 atgttccatg ccgccaataa tctgtgcgcg gtgaatatgc ccgagcgcga tgtagtcggc
    15001 tggtggaaag ttttgtgccg gaaacgcgtc cagcgtgcca atatagatgt cacgcacggc
    15061 atcactttta ctggcaccca cggtcgttaa atgtcccgtg gcgatgatgg gcagaggctg
    15121 atcgccgcgc agtttgcagg catcggcata gtgttgttga taataatcag taatagccgc
    15181 cagcaaatgc tgctgttttt caataccgtt aagccccgct tggctggtaa taatgtcacg
    15241 tgggcgtaaa aacggaatgg ggcacagtac tgcgcctggt gtcccgtcgc gacgaggcaa
    15301 gatttgcggc gcatgtccgg cgctggcgac cacggttgta ttgaggaacg ccatgatatc
    15361 gcgcgattca ttcagcgtgg cgaccgagtc atggtttcct gccagtacca ccagatgaca
    15421 gccagtttgc tgtaaattga caacaaaacg gttgtataac gtgcgggcgt aactgggcgg
    15481 cgagccggta tcgaaaacat caccggcaac aataatcgca tccacctgat gggcttgtgc
    15541 tgtctccagc agccagtcaa gaaaagcctg atgttcggct tcgcggcttt tactgtagaa
    15601 gttctggccg agatgccagt ctgaggtgtg aaggatgcgc ataacggttc cctggcgaaa
    15661 aagcatgggc gcgattatac ccaaacagat gtgccatttg cttttttctg cgccacggaa
    15721 atcaataacc tgaagatatg tgcgacgagc ttttcataaa tctgtcataa atctgacgca
    15781 taatgacgtc gcattaatga tcgcaaccta tttattacaa cagggcaaat catggcgaga
    15841 cgtattctgg tcgtagaaga tgaagctcca attcgcgaaa tggtctgctt cgtgctcgaa
    15901 caaaatggct ttcagccggt cgaagcggaa gattatgaca gtgctgtgaa tcaactgaat
    15961 gaaccctggc cggatttaat tctccttgac tggatgttac ctggcggctc cggtatccag
    16021 ttcatcaaac acctcaagcg cgagtcgatg acccgggata ttccagtggt gatgttgacc
    16081 gccagagggg aagaagaaga tcgcgtgcgc ggccttgaaa ccggcgcgga tgactatatc
    16141 accaagccgt tttcgccgaa ggagctggtg gcgcgaatca aagcggtaat gcgccgtatt
    16201 tcgccaatgg cggtggaaga ggtgatcgag atgcaggggc taagcctcga cccgacatct
    16261 caccgggtga tggcgggcga agagccactg gagatggggc cgacagaatt taaactgctg
    16321 cactttttta tgacgcatcc tgagcgcgtg tacagccgcg agcagctgtt aaaccacgtc
    16381 tggggaacta acgtgtatgt ggaagaccgc acggtcgatg tccacattcg tcgcctgcgt
    16441 aaagcactgg agcccggcgg gcatgaccgc atggtgcaga ccgtgcgcgg tacaggatat
    16501 cgtttttcaa cccgctttta acgccttgct catcggacgc agagcagggc ttatgatttc
    16561 ttaactggag tatcttacgt gctggaacgg ctgtcgtgga aaaggctggt gctggagctg
    16621 ctactttgct gcctcccggc tttcatcctg ggtgcatttt ttggttacct gccctggttt
    16681 ttgctggcat cggtaacagg actgcttatc tggcatttct ggaatttatt gcgcctttca
    16741 tggtggctgt gggtggatcg cagtatgacc ccgccaccgg ggcgtggtag ctgggaaccg
    16801 ctactatacg gcttacacca gatgcagctg cgaaataaaa aacgccgccg cgaactgggc
    16861 aatctgatta aacgctttcg tagtggcgcg gagtcgctgc ccgacgcagt agtgctgacc
    16921 acggaagagg gcggtatttt ctggtgtaac ggtctggcgc aacagattct tggtttgcgc
    16981 tggccggaag ataacgggca gaacatcctt aacctgctgc gttacccgga gtttacgcaa
    17041 tatctgaaaa cgcgtgattt ttctcgcccg cttaatctgg tgctcaacac cgggcggcat
    17101 ctggaaattc gcgttatgcc ttatacccac aaacagttgc tgatggtggc gcgtgatgtc
    17161 acgcaaatgc atcaactgga aggggcgcgg cgtaactttt ttgccaacgt gagccatgag
    17221 ttacgtacgc cattgaccgt gttacagggt tacctggaga tgatggatga gcagccgctg
    17281 gaaggcgcgg tacgcgaaaa agcgttgcac accatgcgcg agcagaccca gcggatggaa
    17341 gggttggtga agcaattgct gacgctgtcg aaaatagaag ccgcaccgac gcatttgctc
    17401 aatgaaaagg ttgatgtgcc gatgatgctg cgcgtcgttg agcgcgaggc tcagactctg
    17461 agtcagaaaa aacagacatt tacctttgag atagataacg gcctcaaggt gtcaggcaac
    17521 gaagatcagc tacgcagtgc gatttcgaac ctggtctata acgccgtgaa tcatacgccg
    17581 gaaggcacgc atatcaccgt acgctggcag cgagtgccgc acggtgccga atttagtgtt
    17641 gaagataacg gaccgggcat tgcaccggag catattccgc gcctgaccga gcgtttttat
    17701 cgcgttgata aagcgcgttc ccggcaaacc ggtggtagcg gattagggtt agcgatcgtg
    17761 aaacatgcgg tgaatcatca cgaaagtcgc ctgaatattg agagtacagt aggaaaagtt
    17821 acacgtttca gttttgttat cccggaacgt ttaattgcca aaaacagcga ttaattcgcc
    17881 tttgtcatct tttattgcca taagccagcc gatgctggct tattttcttt gcagtcgaaa
    17941 tacgggcgtt agattttaca acggttggtg attttttgtt cgcatgatta gccatgtctt
    18001 tttcattgaa atagtgtttt atactggttg gtgatttctt atcgctatat acctctggtt
    18061 tttagatccc ttcttgcttt aaaacgttat aagcgtttaa attgcgcttc aggtgctgtc
    18121 atactgactg cattaacgcg gtaaatcgaa aaactattct tcgccgcgcc tggttgggag
    18181 tatttcccgc taaaattgtt taaatatacc gctgtatcat ccccagggat tggcacaaaa
    18241 atttaacgtt acaacaccac atccacaggc agtatgattt atgacccatc aattaagatc
    18301 gcgcgatatc atcgctctgg gctttatgac atttgcgttg ttcgtcggcg caggtaacat
    18361 tattttccct ccaatggtcg gcttgcaggc aggcgaacac gtctggactg cggcattcgg
    18421 cttcctcatt actgccgttg gcctgccggt attaacggtg gtggcgctgg caaaagttgg
    18481 cggcggtgtt gacagcctca gcacgccaat cggtaaagtt gctggcgtac tgctggcaac
    18541 ggtgtgttac ctggcggtgg ggccgctgtt cgctacgccg cgtacagcta ccgtttcttt
    18601 tgaagtgggg attgcgccgc tgacgggtga ttccgcactg ccgctgttta tttacagcct
    18661 ggtctatttc gctatcgtta ttctggtttc gctctatccg ggcaagctgc tggataccgt
    18721 gggcaacttc cttgcgccgc tgaaaattat cgcgctggtc atcctgtctg ttgccgcaat
    18781 tatctggccg gcgggttcta tcagcacggc tactgaggct tatcaaaacg ctgcgttttc
    18841 taacggcttc gtcaacggct atctgaccat ggatacgctg ggcgcaatgg tgtttggtat
    18901 cgttattgtt aacgcggcgc gttctcgtgg cgttaccgaa gcgcgtctgc tgacccgtta
    18961 taccgtctgg gctggcctga tggcgggtgt tggtctgact ctgctgtacc tggcgctgtt
    19021 ccgtctgggt tcagacagcg cgtcgctggt cgatcagtct gcaaacggtg cggcgatcct
    19081 gcatgcttac gttcagcata cctttggcgg cggcggtagc ttcctgctgg cggcgttaat
    19141 cttcatcgcc tgcctggtca cggcggttgg cctgacctgt gcttgtgcag aattcttcgc
    19201 ccagtacgta ccgctctctt atcgtacgct ggtgtttatc ctcggcggct tctcgatggt
    19261 ggtgtctaac ctcggcttga gtcagctgat tcagatctct gtaccggtgc tgaccgccat
    19321 ttatccgccg tgtatcgcac tggttgtatt aagttttaca cgctcatggt ggcataattc
    19381 gtcccgcgtg attgctccgc cgatgtttat cagcctgctt tttggtattc tcgacgggat
    19441 caaagcatct gcattcagcg atatcttacc gtcctgggcg cagcgtttac cgctggccga
    19501 acaaggtctg gcgtggttaa tgccaacagt ggtgatggtg gttctggcca ttatctggga
    19561 tcgcgcggca ggtcgtcagg tgacctccag cgctcactaa atcactgaac atttgtttta
    19621 accacggggc tgcgatgccc cgtggttttt tattgtgttg atgggttagg aattgatgga
    19681 aagtaagaac aagctaaagc gtgggctaag tacccgccac atacgcttta tggcactggg
    19741 ttcagcaatt ggcaccgggt tgttttacgg ttcggcagac gccatcaaaa tggccggtcc
    19801 gagcgtgttg ttggcctata ttatcggtgg tgtcgcggcg tatatcatta tgcgcgcgct
    19861 gggggaaatg tcggtacata acccggccgc cagctctttc tcgcgttatg cgcaggaaaa
    19921 cctcggcccg ctggctggtt acattaccgg ctggacctac tgctttgaaa tccttattgt
    19981 cgccatcgcc gatgtgaccg cttttggtat ctatatgggt gtctggttcc cgacggtgcc
    20041 gcactggatt tgggtactga gcgtggtgct gatcatttgc gccgtaaacc tgatgagcgt
    20101 gaaggtattc ggtgagctgg agttctggtt ctcgttcttt aaagtcgcca ccatcatcat
    20161 catgattgtc gccggtttcg gcatcatcat ctgggggatt ggcaacggcg ggcaaccgac
    20221 cggtattcat aacctgtgga gcaacggcgg cttcttcagt aacggctggc ttgggatggt
    20281 gatgtcgttg caaatggtga tgtttgctta cggtgggatc gaaattatcg ggattaccgc
    20341 cggtgaagcg aaagatcctg agaaatcgat tccgcgtgcg attaactccg tgccgatgcg
    20401 tattctggtg ttctacgtcg gtacgctgtt cgtcattatg tctatctacc cgtggaatca
    20461 ggttggcact gccggtagcc cgttcgtact gacgttccag catatgggca ttacctttgc
    20521 cgccagcatt cttaactttg ttgtgctgac cgcttcgctg tcggcaatta acagtgacgt
    20581 atttggcgta ggccgtatgc tccacggtat ggcagagcag ggcagcgcac caaaaatttt
    20641 cagcaaaacc tcgcgtcgcg gtattccgtg ggttacggtg ctggtgatga ctaccgcgct
    20701 gctgtttgct gtgtatctga actacatcat gccggaaaac gtcttcctgg tgatcgcttc
    20761 gctggcaacc ttcgccacgg tgtgggtgtg gattatgatc ctgctgtcgc aaatcgcctt
    20821 ccgtcgccgt ttaccgccag aagaagttaa gtcgctgaaa tttaaagtac cgggtggggt
    20881 agcaaccacc atcggcggtt tgattttcct gctctttatt atcgggttga ttggttatca
    20941 cccggatact cgtatctcgc tgtatgtcgg tttcgcgtgg attgttgtgc tgttgattgg
    21001 ctggatgttt aagcgccgcc acgatcgtca gctggctgaa aaccactaat ccttgcgttc
    21061 tgtaagccgg ttcaggagga aatcctgatc cggctattcc gaaagttatc cgcccccgtc
    21121 ctcctccccc aaatatcctt cagatgatga gtgatcctgc attaggctat ggcaaggtga
    21181 tcagattttc atcacagggg aattatgatg ttaaatgcat ggcacctgcc ggtgccccca
    21241 tttgttaaac aaagcaaaga tcaactgctc attacactgt ggctgacggg cgaagaccca
    21301 ccgcagcgca ttatgctgcg tacagaacac gacaacgaag aaacgtcagt accgatgcat
    21361 aagcagcgca gtcagccgca gcctggtgtt accgcatggc gggcggcgat cgatctctcc
    21421 ggcggactac ctcggcggcg ttatagtttc aaattactgt ggcacgatcg ccagcgttgg
    21481 tttacaccgc agggcttcag ccgaatgccg ccggcacgac tggagcagtt tgccgtcgat
    21541 gtaccggata tcggcccaca atgggctgcg gatcagattt tttatcagat cttccctgat
    21601 cgtttcgccc gtagtcttcc tcgtgaagct gaacaggatc atgtctatta ccatcacgca
    21661 gccggacaag agatcatctt gcgtgactgg gatgaaccgg tcacggcgca ggcgggcgga
    21721 tcaacgttct atggcggcga tctagacggg ataagcgaaa aactgccgta tctgaaaaaa
    21781 cttggcgtga cggcgctgta tctcaatccg gtgtttaaag ctcccagcgt acataaatac
    21841 gataccgagg attatcgcca tgtcgatccg cagtttggcg gtgatggggc gttgttgcgt
    21901 ttgcgacaca atacgcagca gctgggaatg cggctggtgc tggacggcgt gtttaaccac
    21961 agtggcgatt cccatgcctg gtttgacaga cacaatcgcg ggacgggtgg tgcttgtcac
    22021 aaccccgaat cgccctggcg cgactggtac tcgtttagtg acgatggcac ggcgctcgac
    22081 tggctgggct atgccagctt gccgaaactg gattatcagt cggaaagtct ggtgaatgaa
    22141 atttatcgtg gggaagacag tatcgtccgc cactggctga aagcgccgtg gaatatggac
    22201 ggctggcggc tggatgtggt gcatatgctg ggggaagcgg gtggggcgcg caataatttg
    22261 cagcacgtag ccgggatcac cgaagcggcg aaagaaaccc agccggaagc gtatatcgtt
    22321 ggagagcatt ttggcgatgc acggcaatgg ttacaggccg atgtggaaga tgccgccatg
    22381 aactatcgcg gcttcacatt cccgttgtgg ggatttcttg ccaataccga catctcttac
    22441 gatccgcagc aaattgacgc ccaaacctgt atggcctgga tggataatta ccgcgccgga
    22501 ctttctcatc aacagcaact gcgtatgttt aatcagctcg atagccacga tactgcgcga
    22561 tttaaaacgc tgctcggtcg ggatattgcg cgcctgccgc tggcggtggt ctggttgttc
    22621 acctggcctg gtgtaccgtg catttattac ggtgatgaag tgggactgga tggtaaaaac
    22681 gatccgtttt gccgtaaacc gttcccctgg caggtggaaa agcaggatac ggcgttattc
    22741 gcgctgtacc agcgaatgat tgcgctgcgt aagaaaagtc aggcgctgcg tcgtggcggc
    22801 tgtcaggtac tgtatgcgga agataacgtg gtggtatttg tccgcgtgct gtatcagcag
    22861 cgtgtactgg tggcaatcaa ccgtggcgaa gcctgtgaag tggtgctacc cgcgtcgccg
    22921 ttgcttaatg tcgcgcaatg gcaacgcaaa gaaggtcacg ggcaactgac agacgggatt
    22981 ctggctttgc ctgccatttc ggctacggtg tggatgaatt aacgtcttat aacgccttgc
    23041 gtgacgcctg ctccatcatc cgcggataaa actgccagaa gcaggtttcg agagcatcgt
    23101 aatgggcgtc taaatcgtac caggagttac gcagggcatc cagacgtggg cgacggcttg
    23161 ccatgccgtt taacacgctc tggatgaaat ccatatcgcg atagcgcacc aaccactgct
    23221 ctgaccataa gtagttgttc agattgataa agcgtggcgg tgagtctgga agaatagtca
    23281 tcacttgctc ccgggcataa caggtaaatt cctgtagcgg aaaatcgggc gacagctgcg
    23341 accagtggcg ggacagaaag tgatcccaca tcacatccag cgtaataggc gcgacacggc
    23401 gcgtttcatt acgaaaccac tcccgcgctt cgcggacttc tggcagattg tcggttaata
    23461 cgtcgattcg ccgatgcata tgaatgccag ccacgacgtc gggcggaaaa ctttcttcag
    23521 gatttccgcg tacgaaatca gccagtaaat tgccggaaag cgagctttcc gcgagatggg
    23581 ctaaatgcag gtgagctaaa aaattcatcg attatattct atccaaaggg gggtaaaggt
    23641 tgcagggaga gcgccccggc actagactac ccgcctctta ttttagtctg agtcagtgtc
    23701 atgcgcgtta ccgatttctc ttttgaattg cccgaatccc tgattgccca ctatcccatg
    23761 cctgaacgca gtagctgtcg tttactgtcg ctggacgggc cgacgggcgc gctgacgcac
    23821 ggtactttca ccgatttact cgataagctc aaccccggcg atcttctggt ttttaataat
    23881 acccgcgtga tcccggcgcg tctgtttggg cgtaaagcca gcggcggcaa gattgaagtg
    23941 ctggttgagc ggatgctcga cgacaaacgc attcttgcgc atattcgcgc ctcgaaagcg
    24001 ccaaaacctg gcgcagaact gctgctgggc gatgacgaac gtattaacgc aacaatgacc
    24061 gcgcgccacg gcgcactgtt tgaagtcgaa tttaatgatc aacgttcggt gctggatatt
    24121 ctcaacagca tcggccatat gccgctgccg ccgtacatcg accgtccgga cgaagatgca
    24181 gaccgcgaac tttatcaaac ggtttatagc gaaaaacccg gcgcggtagc agcaccgacc
    24241 gccggactgc actttgacga acctttgctg gaaaaattac gcgccaaagg cgtggagatg
    24301 gcgtttgtga cgttgcacgt tggtgcgggc accttccagc cggtgcgcgt cgacaccatt
    24361 gaagatcaca tcatgcactc ggaatacgct gaagtgccgc aggatgtggt agacgcggta
    24421 ctggcggcga aagcgcgcgg taaccgggtg attgcggttg gtaccacctc agtacgttcg
    24481 ctggaaagtg cggctcaggc agcgaaaaac gatctcattg aaccgttctt tgacgatacc
    24541 caaatcttta tctatccggg tttccagtac aaagtggtcg atgcgctggt gacgaacttc
    24601 caccttccgg aatcgacgct gattatgctg gtttcggcat ttgctggtta tcaacacacc
    24661 atgaatgcct ataaagcggc ggtagaagag aaatatcgct tttttagtta cggtgatgcg
    24721 atgtttatca cgtacaatcc gcaggcaatt aatgagcgcg ttggggagta attccgcggc
    24781 gctggtttaa acgttggact gtttttctga cgtagtggag aaaaaatgaa atttgaactg
    24841 gacaccaccg acggtcgcgc acgccgtggc cgcctggtct ttgatcgtgg cgtagtggaa
    24901 acgccttgtt ttatgcctgt tggcacttac ggcaccgtaa aagggatgac gccggaagaa
    24961 gttgaagcca ctggcgcaca aattattctc ggtaacacct tccacctgtg gctgcgcccg
    25021 ggccaggaaa tcatgaaact gcatggcgat ttacacgatt ttatgcagtg gaaagggccg
    25081 attcttaccg actccggcgg cttccaggtc ttcagccttg gtgatattcg taaaatcacc
    25141 gaacagggcg tgcacttccg taacccgatc aacggcgacc cgattttcct cgacccggaa
    25201 aagtcgatgg agattcagta cgatcttggt tcggatatcg tcatgatctt tgatgagtgt
    25261 acgccgtatc ctgctgactg ggattacgca aaacgctcca tggagatgtc tctgcgttgg
    25321 gcgaagcgta gccgtgagcg ttttgacagt ctcggaaaca aaaatgcact gtttggtatc
    25381 attcagggca gcgtttacga agatttacgt gatatttctg ttaaaggtct ggtagatatc
    25441 ggttttgatg gctacgctgt cggcggtctg gctgtgggtg agccgaaagc agatatgcac
    25501 cgcattctgg agcatgtatg cccgcaaatt ccggcagaca aaccgcgtta cctgatgggc
    25561 gttggtaaac cagaagacct ggttgaaggc gtgcgtcgcg gtatcgatat gtttgactgc
    25621 gtaatgccaa cccgcaacgc ccgaaatggt catttgttcg tgaccgatgg cgtggtgaaa
    25681 atccgcaatg cgaagtataa gagcgatact ggcccactcg atcctgagtg tgattgctac
    25741 acctgtcgca attattcacg cgcttacttg catcatctcg accgttgcaa cgaaatatta
    25801 ggcgcgcgac tcaataccat tcataacctt cgttactacc agcgtttgat ggcgggttta
    25861 cgcaaggcta ttgaagaggg taaattagag agcttcgtaa ctgattttta ccagcgtcag
    25921 gggcgagaag taccaccttt gaacgttgat taatattaat aatgagggaa atttaatgag
    25981 cttttttatt tctgatgcgg tagcggcaac gggtgcatcg gcgcaaggta gcccgatgtc
    26041 tttgattttg atgctggtgg tattcggtct gattttctat ttcatgatcc tgcgtccaca
    26101 gcagaagcgc accaaagaac acaaaaagct gatggactcc atcgctaaag gtgatgaagt
    26161 gctgacgaac ggtggcctgg tcggtcgcgt aaccaaagta gcggaaaacg gctacattgc
    26221 tatcgcgctg aatgacacca ctgaagtagt tattaaacgt gacttcgtag ctgccgttct
    26281 gccgaaaggc accatgaagg cgctgtaatt aaaatttttc cctaagggaa ttgccgtgtt
    26341 aaaccgttat cctttgtgga agtacgtcat gctgatcgtg gtgattgtca tcggtctgct
    26401 gtatgcgctt cccaacctgt ttggtgagga tccggctgtt cagatcactg gtgcgcgcgg
    26461 agtcgccgcc agtgagcaaa cgctgatcca ggtccagaaa acgttacaag aagaaaaaat
    26521 aactgctaag tctgtggcac tggaagaggg cgctattctt gcgcgcttcg actccactga
    26581 cacccagctg cgcgctcgtg aagcattaat gggcgttatg ggtgacaaat acgtcgtggc
    26641 gcttaacctt gccccggcaa cgccgcgctg gctggcagct attcacgctg agccgatgaa
    26701 gctcggcctt gacctgcgtg gcggcgttca cttcctgatg gaagtggata tggacaccgc
    26761 gcttggcaaa ctccaggaac aaaacatcga tagcctgcgc agcgacctgc gcgaaaaggg
    26821 catcccgtat accaccgttc gtaaagaaaa caactacggc ctgagcatca ctttccgcga
    26881 tgctaaagct cgtgatgaag ccattgcgta tctgagcaag cgccatccgg acctggtgat
    26941 tagcagccag ggcagcaacc agctgcgtgc ggtaatgagc gatgctcgcc tgagtgaagc
    27001 gcgtgaatat gcggtgcagc agaacattaa tatcctgcgt aaccgtgtaa accaacttgg
    27061 cgtggcggag ccggtggttc agcgtcaggg tgctgaccgt atcgttgttg aactgccagg
    27121 tattcaggac acagcgcgtg cgaaagagat tctgggtgcg acggcaacgc tggaattccg
    27181 tctggtaaac accaacgttg atcaggccgc tgcggcatcc ggtcgcgtac cgggcgactc
    27241 tgaagtgaaa cagacccgcg aaggtcagcc ggttgtgctg tacaaacgcg taattctgac
    27301 cggtgaccat atcaccgact ccacttccag ccaggacgaa tacaaccagc cgcaggttaa
    27361 catctcgctc gatagcgcgg gtggtaacat catgtctaac ttcaccaagg acaacatcgg
    27421 caaaccgatg gcaaccctgt ttgtggagta caaagacagc ggtaagaaag atgcaaacgg
    27481 tcgtgcggtt ctggtgaaac aggaagaggt gattaatatc gccaacatcc agtctcgtct
    27541 gggtaacagc ttccgtatta ctggcatcaa taacccaagc gaagcgcgtc agctttcact
    27601 gctgctgcgt gccggtgcgt tgatcgcgcc gattcagatt gttgaagaac gtaccattgg
    27661 cccaaccctg ggtatgcaga acattgagca ggggctggag gcttgccttg ccggtctgct
    27721 ggtttctatt ctgttcatga tcatcttcta taagaagttt ggtctgattg cgaccagtgc
    27781 tctgattgcc aacttgatct tgattgtcgg tattatgtcg ctgttgccag gcgcaacgct
    27841 gagtatgcca ggtatcgcgg gtatcgtctt aacccttgcg gtagcggtcg atgcgaacgt
    27901 actgatcaac gaacgtatta aagaagagtt gagcaacgga cgtactgttc aacaggcaat
    27961 tgatgaaggt tatcgtggcg cattcagttc tatcttcgat gcgaacatca ccacgctgat
    28021 taaagtcatc atcctgtacg cagtgggtac cggggcaatt aaagggttcg cgattactac
    28081 cggtatcggt gtggcgacgt cgatgtttac cgcgattgtc ggtacgcgtg ccatcgtaaa
    28141 cctgctatat ggcggcaagc gcgtcaagaa gctgtcaatc tgaggagtgc gatgtggcac
    28201 aggaatatac tgttgaacaa ctaaaccacg gccgtaaagt ctatgacttt atgcgctggg
    28261 actactgggc ttttggcatc tctggtctgc tgttaatcgc cgctatcgtt attatgggcg
    28321 tgcgcggctt taactggggg ctggatttca ccggtggtac ggttattgaa attacgctcg
    28381 aaaaaccggc tgaaattgac gtaatgcgtg atgcattgca aaaagccggt tttgaagagc
    28441 cgatgctgca aaactttggt agcagccacg acatcatggt ccgtatgccg cctgctgaag
    28501 gcgaaaccgg cggtcaggtg ctgggcagcc aggtcctgaa ggtgattaac gaatccacca
    28561 atcagaatgc ggcagtgaag cgtattgagt tcgtcggtcc gagcgtgggg gcagaccttg
    28621 cgcaaaccgg tgcgatggcg ttgatggcgg cgctgctgtc tatcctcgtg tacgtaggtt
    28681 tccgctttga gtggcgactg gcggcagggg ttgttattgc gctggcacac gacgttatca
    28741 ttacgctggg tattttgtcg ttattccata tcgagattga cctgaccatt gtggcatcgt
    28801 tgatgtcagt tatcggttac tcgcttaacg acagtatcgt ggtatcggac cgtattcgtg
    28861 aaaacttccg caagatccgt cgcggtacgc cttacgaaat ctttaacgtg tccttgaccc
    28921 agacgctgca ccgtaccttg atcacatccg gtactacctt gatggttatc ctgatgctgt
    28981 acctcttcgg tggtccggta ctggaaggct tctcgctgac catgcttatc ggtgtttcca
    29041 tcggtactgc atcttccatc tatgtggcat cggcgctggc tctgaaactg ggtatgaagc
    29101 gcgaacacat gttgcagcag aaagtggaaa aagaaggggc ggatcagccg tcaattctgc
    29161 cttaatcaag tttcagctga tgttgaaaat cccggtcaga agatcgggat tttttttgat
    29221 gtaaggtgat tgcgaaaatc cccgcatctt gggaaactgc gcgtaaccct acatttcatc
    29281 caggtaactt tttcatggct atcatcccaa aaaactatgc gcggttagaa agcggctatc
    29341 gcgaaaaagc attaaaaatc tatccgtggg tctgcggtcg ctgttcccgc gagtttgttt
    29401 attccaacct gcgtgaactt accgttcacc acattgatca cgaccatact aataacccgg
    29461 aagatggcag taactgggaa ttactttgtc tctattgcca cgatcatgag cattcgaaat
    29521 acaccgaagc ggatcagtat ggtacgaccg ttatcgcagg ggaagatgcg cagaaagatg
    29581 tcggtgaagc gaagtacaac ccattcgctg atttgaaagc gatgatgaac aagaagaagt
    29641 gataaaaaat gccggatatg aaatccggca ttctcacaat tagaagttgt aacctactac
    29701 cagataacca ccccagccgg tagagcgaac gttgaagttg ccgttgccga agttcagttc
    29761 tgcatcgtca ttccactgac caccgttatg ccagtaacgg gcaacgacag agtagtgcca
    29821 gtgatcgtaa ttcagcgcca gaatatggct ggacgcaata gagttattgg tacgtgtttt
    29881 aataccgttg ttcgcataac cgctgtcatc gcccaggtcg gaaccccagt cgaagttggt
    29941 gaagccgatg tagctcagct ggccgcccca cagatcggta atcggcacaa agtacttcac
    30001 tttgaaacgg taaccgtccc actcgttttc gttcgctgcg ccgtagttct gccactggta
    30061 tttcgcatag acgttcatgg acaggctcat cggcagacca gtatcgatgt cggtacccag
    30121 acccatgtac caggtgctct ggcggccatc tttattacga cccatgtcgt aaatgtagtt
    30181 gttcgcgaag taccactctt tgaacggacc aaagctaagg tcagtattgg tcagcttgtc
    30241 gatggagaaa cgcggttcga tttccataaa cagcggagaa ccgtggttcc agataccttt
    30301 tgcatcggag ttaccgccga agaataccgg cgcatccgca taaccataga agtcgaacca
    30361 gtctttttta gcgaatgctt cgtactcaag gtaggtatcg ttgcggagct gcggtccgaa
    30421 acgggtgtga tagcttccga caacgttaac gctctggtgc caccagtcgg aaagatactg
    30481 cggtttgtcg ttttcagctg cgttgacagt aaaagacgaa gagagcgcca gtaccgcacc
    30541 ggctgccagt aatgtttttt tcatatgtat gccactgttt gaaaatccct tgcgggagtg
    30601 aaaaaggcgc aaattgcgtt tctaaatatt tcgtatacag aggagcctat tataaaaatc
    30661 attgctcaca aaaatatgtt tcgtttcaca gttctatcat ttacgtaatc gattgcgttc
    30721 acgtttacac acattcgggc ggggattgta ctgactttca cccttgttgc aaacttttac
    30781 tatcaatcca ggtgatgtaa gcggagtaac aaaatgacgg agaaagatgt tgcgggacgc
    30841 acaatatcgc cccgcaatac gtttactgca taacaggttg aatgtcgtgg atacgtacaa
    30901 agcctaactg atccacagac atacccttta actgtaacgg aatatcaaca tcgctggggg
    30961 cgaggacgct ggcaggtgcg ttaatcaatt gattctgcac attgatttcc tgaaagttgt
    31021 ctattgttcc ctgaatttgc ccatattcaa cggttccgct gaatgcgggt aaagggtcat
    31081 tggattcgcc ctgaatacgt agcgtcaggg tagtgccatc tgtgtcaggc gtaatattca
    31141 ccagcgacat acgtaaagta ccgatctggc tttccagtct tgctggtgtt tttgctccag
    31201 gcagaaggta aaccccactg ctggattttg cattcagctt gttttgctga gtgattttga
    31261 ccgtttcttt attgagttgc gtcatctcct ggttcagcgt gctgacgctg tgtttcatct
    31321 gtcgcacttc actttgctgc acacaggcgc taaggctgaa caggcttccc agcaggagca
    31381 gtcgaaaaac gttagtattc ataggtcgtc cctttctcgt cgcaattagc ctaatggtag
    31441 agggtaaaag cagtgaaagc atcgatcctt tatctcaaaa gcgttgcgcc tttgttgtat
    31501 cgtcagttca gggtaaaata gatttctgtt aaccacctgg tcaggacgcc gtatgcattg
    31561 cccattctgt ttcgccgtgg acactaaggt aattgactct cgtctcgtgg gcgagggttc
    31621 atccgtacgc cgccgtcggc agtgtctggt gtgtaatgaa cgtttcacca cctttgaagt
    31681 ggcggagctg gttatgccgc gtgttgtaaa aagcaacgac gtgcgtgaac cgtttaatga
    31741 agagaaatta cgtagcggaa tgctgcgggc gctggaaaaa cgtccggtga gttccgatga
    31801 cgtcgaaatg gcaatcaatc atataaaatc gcagctgcgc gccaccggtg agcgcgaagt
    31861 gccgagcaag atgattggca atctggtgat ggagcaattg aaaaagctcg ataaagtcgc
    31921 ctatatccgt tttgcctctg tctaccgcag tttcgaagat atcaaagaat ttggcgaaga
    31981 gatcgcgcgc ctggaggact aagccgtgca ggacgagtat tacatggcgc gggcgctaaa
    32041 gctggcgcaa cgaggacgtt ttaccacgca tcccaacccg aatgtcgggt gcgtcattgt
    32101 caaagatggc gaaattgtcg gtgaaggtta ccatcaacgc gcgggtgaac cgcatgccga
    32161 ggtacacgcg ttgcgcatgg ccggcgaaaa agccaaaggc gcaacggcgt atgtcacact
    32221 cgaaccctgt agccatcatg gtcgtacgcc accgtgttgt gatgcattaa ttgctgcggg
    32281 cgtggcgcgc gtggttgctg caatgcaaga ccccaatccg caggtcgctg gacgtggact
    32341 ttaccgtctg caacaggctg gcattgacgt cagccacggg ctgatgatga gtgaagctga
    32401 gcaattgaat aaaggctttc tcaaacggat gcgcaccggc tttccttata ttcagttaaa
    32461 acttggcgca tcgcttgatg gtcgcacggc gatggcgagt ggcgaaagcc agtggatcac
    32521 ttcacctcag gcgaggcgcg acgtacaacg actgcgcgcg caaagtcatg ccattttaac
    32581 cagcagcgcc acggtgctgg cggatgatcc agccttaaca gtgcgttggt ctgaactgga
    32641 tgaacaaact cagggactct acccgcaaca aaatctccgc cagccggtac gtattgtgat
    32701 tgatagccaa aatcgcgtga cgccggaaca tcgcattgtg cagcagcccg gcgaaacctg
    32761 gttcgcgcgt acgcaggaag attctcgtga gtggccggaa acggtgcgta ccttgctgat
    32821 tccagagcat aaaggtcacc tggatctggt tgtactaatg atgcaactgg gtaaacagca
    32881 gattaacagc atctgggtgg aagcggggcc aacgctcgct ggcgcattgc tacaggcggg
    32941 tttagtcgat gagctgattg tctatatcgc acctaaacta ttaggcaacg acgcccgtgg
    33001 attatgcacg ctgccagggc ttgagaaatt agccgacgct ccccaattta aattcaaaga
    33061 gatacgtcat gtaggcccgg atgtttgcct gcatttagtg ggtgcatgat ctcccggctc
    33121 gaaagggaag cagcgcacga aatattatgc taaaatccgc ccccctgcgg ggccatactc
    33181 gaacccgaag gaagaaaatg aacattattg aagctaacgt tgctaccccg gacgctcgcg
    33241 tcgccatcac catcgcgcgt ttcaacaact ttatcaatga cagcctgctg gaaggtgcaa
    33301 ttgacgcact gaaacgtatc ggtcaggtaa aagatgaaaa cattaccgtt gtttgggtgc
    33361 caggtgccta tgagctgccg ctggcggcgg gtgcactggc taaaaccggt aaatacgacg
    33421 cggtgattgc gctgggtacg gttattcgtg gtggcactgc ccactttgaa tatgtcgctg
    33481 gtggtgcaag caacggcctg gcgcatgttg cccaggacag cgaaattccg gttgcttttg
    33541 gggttctgac cactgaaagc attgaacaag cgatcgaacg tgctggcacc aaagctggca
    33601 acaaaggtgc agaagctgca ctgaccgcgc ttgaaatgat taatgtattg aaagccatca
    33661 aggcctgaaa ttagtaaggg gaaatccgtg aaacctgctg ctcgtcgccg cgctcgtgag
    33721 tgtgccgtcc aggcgctcta ctcctggcag ttgtcccaga acgacatcgc tgatgttgaa
    33781 taccagttcc tggctgaaca ggatgtaaaa gacgttgacg tcctgtactt ccgtgagctg
    33841 ctggccgggg tggcgactaa caccgcatac ctcgacggac tgatgaagcc gtacctgtcc
    33901 cgtctgctgg aagaactggg ccaggtagaa aaagcagtac tgcgcattgc gctgtacgag
    33961 ctgtctaaac gtagcgatgt gccatacaaa gtggccatta acgaagcgat cgaactggcg
    34021 aaatcgttcg gcgcagaaga cagccataag ttcgtcaacg gcgtactcga taaagcagca
    34081 cctgtgattc gccctaacaa aaagtaatat ccaggccggt agattcacgg aagaccgttc
    34141 catgatcgcc ggccttttct tttttacctg ctgaggcata acgtatggca tgtggcgagt
    34201 tctccctgat tgcccgttat tttgaccgtg taagaagttc tcgtcttgat gtcgaactgg
    34261 gcatcggcga cgattgcgct cttctcaata tccccgagaa acagaccctg gcgatcagca
    34321 ctgatacgct ggtggcgggc aaccacttcc tccctgatat cgatcctgct gatctggctt
    34381 ataaagcact ggcggtgaac ctaagcgatc tggcagcgat gggggccgat ccggcctggc
    34441 tgacgctggc attaacctta ccggacgtag acgaagcgtg gcttgagtcc ttcagcgaca
    34501 gcttgtttga tcttctcaat tattacgata tgcaactcat tggcggcgat accacgcgtg
    34561 ggccattatc aatgacgttg ggtatccacg gctttgttcc gatgggacga gccttaacgc
    34621 gctctggggc gaaaccgggt gactggatct atgtgaccgg tacaccgggc gatagcgctg
    34681 ccgggctggc gattttgcaa aaccgtttgc aggttgccga tgctaaagat gcggactact
    34741 tgatcaaacg tcatctccgt ccgtcgccgc gtattttaca ggggcaggca ctgcgcgatc
    34801 tggcaaattc agctatcgat ctctctgacg gtctgatttc cgatctcggg catatcgtga
    34861 aagccagcga ctgcggcgca cgtattgacc tggcattgct gccgttttct gatgcgcttt
    34921 ctcgccatgt tgaaccggaa caggcgctgc gttgggcgct ctctggtggt gaagattacg
    34981 agttgtgttt cacggtgccg gaactgaacc gtggcgcgct ggatgtggct ctcggacacc
    35041 tgggcgtacc gtttacctgt atcgggcaaa tgaccgccga tatcgaaggg ctttgtttta
    35101 ttcgtgacgg cgaacctgtc acgttagact ggaaaggata tgaccatttt gccacgccat
    35161 aaagatgtcg cgaaaagtcg cctgaaaatg agtaatccgt ggcatctgct tgctgtcgga
    35221 ttcggaagtg gattaagccc gatcgttcct ggcacgatgg ggtcgctggc ggcgattccc
    35281 ttctggtatc tgatgacttt tttgccctgg cagctctatt cgctggtggt aatgctgggg
    35341 atctgtatcg gcgtctatct ttgtcatcaa acggcgaaag acatgggcgt gcacgatcac
    35401 ggcagtattg tctgggacga atttattggt atgtggatta cgctcatggc gctgccgacc
    35461 aatgactggc agtgggttac cgccggattt gtgattttcc gtattctgga tatgtggaag
    35521 ccgtggccga tccgctggtt tgatcacaat gtacatggcg gcatggggat catgattgac
    35581 gatattgtcg ccggggtgat ctccgctggc atcctgtatt ttatcggtca tcactggccg
    35641 ctgggtattc tgtcgtagtt gtgcaccgat gcctgatgtg acgcttgtca cgtctcatca
    35701 ggcctgcata cctatttaaa tcctacgacc ggatgcggtt tatacggcgt ttccagctcg
    35761 gcgatctgtt ccggcttcaa ggtgatatcc accgcgttta gcagttcatc aagctgttcc
    35821 tcccgagaag ttccgataat cggtgcggca atgcccggtt tactcaacaa ccaggccagc
    35881 gcaacttgtg ctcgcgttgc accaagttct tcactgacgc ccgttaagcg ttctgcaatt
    35941 tgtgcgtcat tttcattgct ttcttggtag agatttttcc ccacttcatc cgacaccagc
    36001 cgtgcggtgg tttctcccca aggacgcgtc agacggcccc gcgccagcgg actccatgga
    36061 atcaccgcca cgccttcctg ataacacagc ggcagcatct cgcgctcttc ttcacgataa
    36121 atcagattgt agtgatcctg catactgaca aactgcgccc agccgtgctg tttttgtagt
    36181 tccagtgcct gagcaaactg cgaagcgtgc atggatgacg cgccgatata acgtgctttc
    36241 ccggctttta ccacgtcgtt cagggcttcc agcgtctctt cgatcggcgt gttgtaatcc
    36301 cagcgatgaa tttgcaggat atcgacataa tccatgccga gacggagcag gctgtcgtcg
    36361 atagagcgca aaatttgtgc acgggataat ccttccggta aatcaccaac gcgatggaac
    36421 actttggtcg caacgaccac gtcttcacga cgggcgaaat cccgcagtgc gcgaccgacg
    36481 atctcttcgc tgctgccatc ggaatagcta ttggcggtat caaagaaatt tatgccgcct
    36541 tccagtgcgc gtttaatgat gggacggctg ctctcttccg gcagtgtcca tgcgtgatta
    36601 ccgcgatctg gctcgccaaa ggtcatacag ccgaggcaaa gtcgggaaac acgaaggtcg
    36661 gtttttccta aggggttgta ttgcatgctg ccactcctgc catgaataat ttcttaagca
    36721 tagcaggagt agagtaggga ttatgccagc caggctttga ttttggcttc cataccggtg
    36781 gcatcgaggc cgagttcggc gcgcatttct tcctgagttc cttgtggaat aaagaagtcc
    36841 ggcagaccaa tgttcagcac gggtactggt ttacgatggg ccatcagcac ttcgttcacg
    36901 ccgctgcctg caccacccat aatggcgttt tcttctacgg taaccagcgc ttcatggctg
    36961 gcggccattt ccagaattaa cgcttcatca agcggtttca cgaaacgcat atcgaccagc
    37021 gtagcgttca gcgattcagc gactttcgcc gcttctggca tcagcgtacc aaagttaagg
    37081 atcgccagtt tctcaccacg acgcttcaca atgcctttgc caattggcag tttttccagt
    37141 ggcgtcagtt ccacgccaac tgcgttgccg cgcgggtagc gcaccgcgga cgggccatcg
    37201 ttatagtgat agccggtata gagcatctgg cgacattcgt tttcatcgct cggggtcata
    37261 atgacaattt ccggtatgca gcgcaggtaa gagagatcaa aagcgccctg gtgggtttga
    37321 ccgtcagcac caacaatgcc cgcacggtcg atggcgaaca ggaccggaag cttttgaatc
    37381 gccacgtcat gcagcacctg atcataggcg cgttgcagga aggtggagta gatcgcaaca
    37441 atgggtttgt agcctccaat cgccagaccc gcagcaaagg tcactgcgtg ttgctcggca
    37501 attgccacgt cgaagtagcg atccgggaat ttacgtgaaa actcgaccat gccggaacct
    37561 tcacgcatcg ccggagtaat cgccatcagc ttgttatctt tcgctgcggt ttcgcacaac
    37621 cagtcgccaa agatttttga atagcttggc aaaccgccgc tacttttcgg cagacaaccg
    37681 ctggagggat caaatttagg cacggcgtgg aaggtgattg ggtctttttc tgccggttca
    37741 taaccacgac cttttttggt catgatatgc aggaactgcg ggcctttcag gtcgcgcatg
    37801 ttcttaagcg tggtgataag ccccagcaca tcgtgaccgt ccaccgggcc gatgtagtta
    37861 aagcccagct cttcaaacaa cgtgccaggc actaccatgc ctttaatatg ttcttcggta
    37921 cgtttgagca gctctttaat gggtggcacg ccagagaaaa ctttttttcc gccttcgcgc
    37981 agtgaagagt aaagcttacc ggaaagcagc tgcgccagat ggttgttgag cgcgccaaca
    38041 ttttcggaaa tcgacatttc attgtcgttg agaaccacca gcatatcagg acggatatcg
    38101 cccgcgtgat tcatcgcttc aaacgccatg ccagcagtaa tcgcgccatc gccaatgaca
    38161 cagacggtgc ggcgattttt gccttctttc tcggcagcaa ctgcgatccc aattccggca
    38221 ctgatggagg ttgatgaatg cccgacgctt aatacgtcat attcgctttc gccgcgccac
    38281 gggaacgggt gcaggccacc tttctgacgg atggtgccga ttttgtcgcg gcgcccggtc
    38341 aaaattttat gcggataagc ctgatgcccc acatcccaga ttaactggtc aaacggggta
    38401 ttgtagacat agtgcagcgc cacggtcagt tcgaccgtgc ccagcccgga ggcgaagtgc
    38461 ccgctggaac ggctcacgct gtcgagtaaa tagcggcgca gttcgtcgca gagtttcggt
    38521 aaactctctt tcggcaacag acgtaactcc tgggtggagt cgaccagtgc cagggtcggg
    38581 tatttggcaa tatcaaaact catcaggggc ctattaatac ttattgttta tttattacgc
    38641 tggatgatgt agtccgctag cgcttccagt gccgaggtat cgagtgactg ttcagccagt
    38701 tgtttcagcg actgacgggc atcgtcgatc agatcccggg ctttcttccg ggcttgctca
    38761 agacccagaa gtgcagggta ggtactttta ccaagttgct ggtcggcacc ctggcgtttt
    38821 cccaacgttg cagtatctcc caccacatcc aggatgtcat cctgaacctg gaaggcaagg
    38881 ccgatgctct ctgcgtactt gtcgagtaca gacagagcac gacgtccttt atctccggcg
    38941 cttaatgcac caaggcgaac ggcggcgcga atcaatgcgc cggttttatg acgatgaata
    39001 cgctcaagcg cctccagagg tacgtgtttg ccttccgcgt ctaaatctaa tgcctgacca
    39061 ccgcacattc cggcaatacc gctggcgctc gccagttcag aaatcatcga aattctgtcg
    39121 cgatccgaca cttccggcat atcagcatcg cttaaaatcg agaacgccag cgtttgtaaa
    39181 gcatcgccag cgagaatcgc gttcgcttcg ccaaacttca catggcaggt cggcaaaccg
    39241 cgacgcagat cgtcatcatc catcgccggt aaatcatcat gaattaatga gtaagcgtgg
    39301 atacactcaa cggcggcagc gggtgcgtcc agcgtgtttg tgctaacgcc gaacatatga
    39361 ccggtggcat aaaccaggaa aggtcgcagg cgcttaccac ctaataatgc gccatactgc
    39421 atggtttcga ccacgggagt gttctgaaag ggcagtgggg cgataaaacg gctcagcgcc
    39481 tggttggcct gcttaacgca ggcttcgagt tgctgcggaa agtccattac tcattgtccg
    39541 gtgtaaaagg ggttagagag gcgtcttcat tgtcagacag cagaatttgc acgcgctgtt
    39601 cggcttgttg taatttggcc tgcccctgac gtgccagttg cacgccgcgt tcgaactcgt
    39661 tcagcgcctc ttccagcggc aggtcgccac tttccagacg ggttacaatc tgttccagct
    39721 cgctcagcgc cttttcaaag ctggcgggcg cctcattttt cttcggcata atgaatgtct
    39781 gactctcaat atttttcgcc ccgtcatggt aacggactca gggcaaatag caaataacgc
    39841 gcaatggtaa ggtgatgtgc gcagcaaagc gatgttagtg gtatacttcc gcgcctggat
    39901 gcagccgcag gtgtgggctg ctgtattttt ccctatacaa gtcgcttaag gcttgccaac
    39961 gaaccattgc cgccatgaag tttatcatta aattgttccc ggaaatcacc atcaaaagcc
    40021 aatctgtgcg cttgcgcttt ataaaaatcc ttaccgggaa cattcgtaac gttttaaagc
    40081 actatgatga gacgctcgct gtcgtccgcc actgggataa catcgaagtt cgcgcaaaag
    40141 atgaaaacca gcgtctggct attcgcgacg ccctgacccg tattccgggc atccaccata
    40201 ttctcgaagt cgaagacgtg ccgtttaccg acatgcacga tattttcgag aaagcgttgg
    40261 ttcagtatcg cgatcagctg gaaggcaaaa ccttctgcgt gcgcgtgaag cgccgtggca
    40321 aacatgattt tagctcgatt gatgtggaac gttacgtcgg cggcggttta aatcagcata
    40381 ttgagtccgc gcgcgtgaag ctgaccaatc cggatgtgac tgtccatctg gaagtggaag
    40441 acgatcgtct cctgctgatt aaaggtcgct acgaaggtat tggcggtttc ccgattggta
    40501 cccaggaaga tgtgctgtcg ctcatttccg gtggttttga ctccggcgtt tccagttata
    40561 tgttgatgcg tcgcggatgt cgtgtgcatt actgcttctt taaccttggc ggcgcggcgc
    40621 atgaaattgg cgtgcgtcag gtggcgcatt atctgtggaa ccgctttggc agctcccacc
    40681 gcgtgcgttt tgtcgctatt aatttcgaac ctgtcgtcgg ggaaattctc gagaaaatcg
    40741 acgacggtca gatgggtgtt atcctcaaac gtatgatggt gcgtgccgcg tctaaagtgg
    40801 ctgaacgtta cggtgtacag gcgctggtca ctggcgaagc actgggccag gtgtccagcc
    40861 agacgctgac taacctgcgc ctgattgata acgtctccga cacgctgatc ctgcgtccgc
    40921 tgatttctta cgacaaagag cacatcatca acctggctcg ccagattggc actgaagact
    40981 ttgcccgcac gatgcccgaa tattgcggtg tgatctccaa aagcccgacg gtgaaagcgg
    41041 ttaaatcgaa gattgaagcg gaagaagaga agttcgactt cagcattctc gataaagtgg
    41101 ttgaggaagc gaataacgtt gatatccgcg aaatcgccca gcagaccgag caggaagtgg
    41161 tggaagtgga aaccgtcaat ggcttcggcc cgaacgacgt gatcctcgat atccgttcta
    41221 tcgatgaaca ggaagataag ccgctgaaag tcgaagggat tgatgtggtt tctctgccgt
    41281 tctataaact gagcaccaaa tttggcgatc tcgaccagaa caaaacctgg ctgttgtggt
    41341 gtgagcgcgg ggtgatgagc cgcctgcagg cgctctatct gcgcgagcag ggctttaaca
    41401 atgtgaaggt atatcgcccg taatttgtgg tttttacgtc gcatctggtc agatgcgacg
    41461 tttgccgcat ccgccactac tcgtaataat tataaatccc tgccgccatc accagttgtg
    41521 atgccacttc atgggctttt tcacgcccaa ccagcaggtc gataattttc agaccaaagt
    41581 cgatagctgt acccggcccc tgactggtca gcaattttac ccgtgcatcc cagacgacgc
    41641 gcttgtcctg ccattgttcg gcgggtattt tgtctttcag cgtcgggaag ccggtcatat
    41701 tgccaatcgg gaagatatcg tgcgggacca gcacggtggc tggtgcggcg caaatagccg
    41761 cgacgatacg cccggaacgg tggaactgtt taacggtttc aaccagcagg gtgctatcgc
    41821 gaaaacactc tgcgccttta atgcctccag gcagcacgat cacgtcatat tcgccatcag
    41881 ctacttcgac cagcggcgca tccgccagca gcttcacgcc gcgcgagcag gtaatcgcca
    41941 ggttgccatc gctggcgacg ctggcagtgg tgactttgat accgccgcga accagcagat
    42001 cgatagtggt gacggcttca gtctcttcac taccaggggc gaggcaaacc agtgccgatg
    42061 cgctcatatt cactctcctt tctttttacc atttcaaaca ggcgggtgtt ttccggtacg
    42121 gtaatcccat gcgcgcgggc gcggcgtaag agaaaaccat tgatatagtc gatttcggtg
    42181 tggcgcagcg cgcggatatc ctgcaacatc gacgagatat tttccgctgt ggcatcaatc
    42241 acctgcatca cgtaatcacg caaatcttct gctgaagtat gatgtccttc gcgttcgatc
    42301 actgccgcga cttcctcgca tatctgcata atttcttgcg gatgatgacg taattcaccg
    42361 ttcgggcaat tccagatggc agtcagtgga ttaatcacac agttgactgc cagcttgcgc
    42421 cacagctcgg cgcgaatatt gttatgccag gcaacgtcag gcaacacggt ttgcaaaata
    42481 tccgccagat aactgtaatc gccatcctgt tgccgtgccg gaccaatatg cgtgataccg
    42541 tttgccacat gaataatgac attgccgtcg cggcgagcgg catgggtggt ggtgcccatc
    42601 agtaatggct gctgaatgtt ttgcaactct tcgatggtgc ccatgccgtt gtgaattaac
    42661 agtattggcg tagttacagg cagtgtggac gcgaggcttt tgacggcatc ggaaacctgc
    42721 catgctttca gcgtcaccag gagcagatcg ctggtggcga gaaaatcggg atcgttggcg
    42781 gtcagcgatt cgttaaatat cgaaccatct gtctcaacca gattcacgct acaataaggt
    42841 tgcggtacgc gcagccagcc ctgaacgtcg tgaccctgtt tacaaagtgc tgtaagccat
    42901 aattgcccta aggcaccgca tcccaatacg gtaattttca ttgttcctcc tcacccgcaa
    42961 ccactccggg tgttcaataa ggctatccct taattgtgca tgctgttgcg actatgcaca
    43021 attaagggat acgtcctggt gcaggactgt tggttattta actttgcagg tattatgctt
    43081 cgcatcaaaa atgaagggag aggaaaagat gccatctttc gatattgtct ctgaagttga
    43141 tcttcaggaa gcacgtaacg cggtcgataa cgcgagccgc gaagtggagt cccgttttga
    43201 cttccgtaat gttgaagcct catttgagct gaacgacgcc agcaaaacca tcaaagtgtt
    43261 gagcgagtcc gatttccagg tcaatcagtt gctggatatt ctgcgtgcca agctgctgaa
    43321 gcgcggcatt gaaggcagtt cgctggatgt accggaaaat atcgttcata gcggtaaaac
    43381 ctggtttgtg gaagcgaaac tgaaacaggg cattgagagc gcgactcaga agaaaatcgt
    43441 caaaatgatc aaagacagca aactgaaagt gcaggcgcaa attcagggcg atgagatccg
    43501 cgtaacgggt aaatctcgtg atgatttgca ggctgtcatg gcgatggtac gtggtggcga
    43561 tctcggtcag ccgttccagt tcaaaaactt ccgcgattaa tcgcgacaac ctgcgctttg
    43621 ttcatgtcag atgcggcatg aacgcctgat ttggcttaca aaggtttgta actcaggaac
    43681 ttagtaggcc tgataagcgt agcgcagcag gcaatttcgc ctcgctttta tgcctggcga
    43741 attgcctgtt caacctcaaa gcgattcgtc actttgctgt cgattttcac atacgctgaa
    43801 tgttcttctt ctgcaatcaa cacttctttg acgccttcag tttccagcaa acgtacttct
    43861 aacgcttcgt ttgcggcagt gtccgccggg atttcaatgc gcaagctgct gacatacggc
    43921 ggttctttca tggtactggc gactgccagc cacactgcgg ccagcattgc gccagcgaga
    43981 aatactccct gaccgtcaaa catgccgtca atccagccgc ccagcgaacc gccaatcgcc
    44041 acgccaagaa actggctggt ggagtaaaca cccatcgccg tacctttgta acctgctggc
    44101 gattctttac tgataagtga aggcagaagg gcttccatca aattaaacgc cacaaagaaa
    44161 agctgcacgc cgaccaccag ttgccagaac tgcgtttgtg cgttccacaa cacaatttcc
    44221 gcaaccacga tcagcccgac gcaaaagaca aagacttgct tcattttgcg cttaacttca
    44281 gcgtagataa tgaaaggcac gaccgagcca aaggcgatta acatcgttgc caggtagacc
    44341 ttccagtgtt cagccgccgg gaaccccgca tcagccatct gtccgggcag ggcaacaaag
    44401 gtcgacatca gcaaaatatg cagacacata atgccaaagt tgagtttcag cagccgcggt
    44461 tccgccagca ctttactgaa acttcctttc accattccgg actcacgatt aagtacgtga
    44521 gtgctactgt tgggcacaac ccaaatggtc aacgcaatgc cggttgctgc cagaatagcg
    44581 atcatccaga acagcgcgtg cagcccaagt ttgtgagtga tgatcgggcc aagcaccatc
    44641 gcaatggcaa aggtaatgcc aaagctcacg ccgataaacg ccatcgcttt ggtgcggttt
    44701 tgttcgcgcg tgagatcgga aagcagcgcc ataacggcag cggcaatcgc accggagcct
    44761 tgtagcgccc ggcccagaat aattccccag atggagtcag agagcgcagc gataacgcta
    44821 ccggcggcaa acactgccag cccaccgaca attaatggtt tgcgaccaat gcggtcggaa
    44881 agcaggccaa acggaatctg aaaaacggcc tgagtcagac cataaatacc aatggcaata
    44941 ccgattaatg cttcgctggc accttgcaga gccatgccgt acgtggtcag aaccggcaga
    45001 accatgaaca tgcccagcat gcgcaacgag aatacggtcc ctaaacccca ggtcgcgcgt
    45061 cgctcacctg gcgtcatttt ataatcgttc attaccacct ctgttttaaa ttcgcgacta
    45121 gtgtaaagcg gcaaacgaat ggggtaaaca tgcggatttt ttagtaaata tttcgagagg
    45181 gatatgattc taatgctaag aaaaaaggtg ccgtagcacc tttttaactg agaggttgtg
    45241 ttaccacaca gcagccagca gcgtatgcga gtccggcacc ataaaatcaa cggacatcat
    45301 caccgagagg gcagtgatgg cgatgataga gaagccgaac agcttgcgcg cccagattct
    45361 gtcatcagca actttataac cgcgcagagc catgcctaac caccagacgc tcaccgccgc
    45421 ggcgactacc agatatttat acccagcgta accgccaaga gagagcatca gcgtggcaac
    45481 ggcaaaggcg atgatataca gcgtgatgtg attcttcgcc accgaaatgc cttttaccac
    45541 tggcaatacc ggaatgtttg ccgcctggta atccttaaag cggaaaatgg cgatggcata
    45601 ggagtgaggc atctgccaca gactgaagat agcaagcagg atcgctgcgc cactatcgaa
    45661 ctcgccggtt actgcacagt agccgatcac cggcggcgca gcgccggaga gcgaaccaat
    45721 caatgtgccg tagactgagt ggcgtttcat gtacaggcta taaacgccga cataaaccac
    45781 aaagcccatc acccccagcc agcaggccag cggattcgcg ccaaaccaca gcagcataaa
    45841 gccagcaata cccagcaagg tggcgtacac cagcgagaca gcaggtgaga tcaggccttt
    45901 aaccagcacc cgattcttcg tcctttccat ctttctgtcg atatccctgt cgatgtagtt
    45961 gttaaacaca caacccgacg ccacaaccag tgacacccca accagcgtgt agataaacag
    46021 gggataatca atgctgccct ttgaggccaa caggaatccc ccaatcaccg agatcaggtt
    46081 gccaaagatg atgcctggtt tcgttacttg caggtattgc ttaaacatca taaccgccgc
    46141 tcttagtgca tcatcatgtt gtagttgagg ttccacataa tccagatgga gcctacaacc
    46201 aggatagcga tgattagcac ggtgaagaca aacgccgtca tgttccagcc ttcatctgat
    46261 ttggtattca tgtgcaggaa gcacaccaga tgcaccagaa cctgtaccac tgccattgcc
    46321 aggattgttc ccagaattac ggccggagag gcagctcctg tcatcaccat ccagaacgga
    46381 atcaccgtca gaatgatcga caggataaag cctgtcatgt aggtttttac gctgccatgg
    46441 gacgcgccgc tgtgatcggt agaatgactc attacatcgc ccccatcaga taaacaacag
    46501 tgaatacaca gatccaaacc acatccagga agtgccagaa caggctcagg cacatgatgc
    46561 gggtacgatt agtgctggtc aggccgcgac gggcgatttg caccatcagc accgccatcc
    46621 agataagacc ggaagtgacg tgcagaccgt gcgtgccgac cagcgcaaag aacgctgaca
    46681 ggaagccgct gcgatccgga cccatgccgt taacaatcag atgatggaat tcatagagct
    46741 ccatcccgat aaatccggcg ccaaacaacc aggtcaacgc cagccaggag acaacctggc
    46801 ttttgttgtt tttgtacatg gcgatagccg ccatgccgta ggtgatggag ctgaacaaca
    46861 gcaggaaagt ttcaaccaga acgaacggca gttcgaaaat gtccttacct gtcgggccgc
    46921 ctgcggtgcc gttcaccaga acggcatagg tagcaaacag gatagagaac agaatgcagt
    46981 cgctcatcag gtagatccag aatccgaaga ctttggttcc gcctgcatcg tggtgcccgt
    47041 gttcgtgcgc gtgggcagtc gcgtgcgtca aagtatcagt tgccattttt cagccctgcc
    47101 ttagtaatct catcgaaatg ctggttttcc agtttttcga tttctgccac cggcacgtag
    47161 taatccacgt cctcgtcgaa gcttttcacg atccaggtga tgatcatgcc tgcgaagcca
    47221 acaatcgcca gccaccagat atgccagatc atggcgaaac cgaagatggt ggagaaagcc
    47281 gcaatgacga tacctgcacc gctgtttttc ggcatatgaa tttcttcata gtggtcaggc
    47341 tttttgtacg cttcgccttt ctctttcatt tcccagaatg catcacgctc gtgaacgtgc
    47401 ggcacaacgg caaagttata gaacggaggc ggggaagagg ttgcccactc cagcgtacgg
    47461 ccaccccacg ggtcgccagt caggtcacgg ttctggtcgc ggtcacgaat agaaacgtac
    47521 atctgaataa cgaggcagag aatacccagc gcaatcagta ctgcaccgct ggctgcaatc
    47581 atcagcatgg tgtggaactg cgggtcaatc tgctggctca aacgacgggt catgcccatg
    47641 aagcccagcg catacagcgg cataaaggca acgaagaagc cgatgatcca gaaccagaac
    47701 gcgcgtttac cccaggtttc gttcagtttg aaaccgaacg ctttaggcca ccagtaggtc
    47761 atccctgcga agcagccgaa gaccacgccg ccgatgatca cgttatggaa gtgcgcaatc
    47821 aggaacaggc tgttatgcag aacgaagtcc gcgcccggta ctgccagcag cacgccagtc
    47881 ataccgccta ccgagaaggt gacgataaaa ccgatggtcc acagcatcgc agaatggaac
    47941 acgatgcggc cctgatacat ggtgaacagc cagttgaaga tcttcacccc ggtcgggatg
    48001 gcgataatca tagtggtgat accaaagaag gcgtttacgt tcgcgcccgc acccatcgta
    48061 aagaagtggt gcagccagac gatgaacgac agcacggtga tacagacggt tgcccatacc
    48121 agcgaggtat aaccaaacag acgtttacgc gagaaggttg ccgcaatttc ggagaacaca
    48181 ccgaaaacag gcaggatcag gatgtaaact tccgggtggc cccaggccca aatcaggttg
    48241 atgtacatca tcatgttgcc acccatatcg ttggtaaaga aatgggtgcc cagatagcga
    48301 tccagggtca acaacgcaac ggtaaccgtc agaattggga aggaagcaat aatcagtacg
    48361 ttcgcgcaca gtgatgccca ggtaaatact ggcatcttga acatggtcat gcccggtgcg
    48421 cgcatcttca gaatggtaac gaagaagttg ataccggtaa gcgttgtacc tataccggat
    48481 agctggagac tccatatcca gtaatcgaca ccgactcccg gactgtactc tattcccgat
    48541 agcggtggat aggccagcca gccggtctgc gcaaattcgc ccacgccgag agaaacgtta
    48601 accagaatca caccaacaac ggtaaaccag aagcttaggt tgttgaggaa cgggaacgca
    48661 acgtcacgcg cgccgatctg cagcggaacc accaggttca tcagaccgat aacgaaaggc
    48721 atcgccacga agaagatcat aatcacgccg tgcgcggtga agatctgatc gtagtggtga
    48781 ggtggcagga agcccgcttc gcccgccgag gcaagagcct gctggctacg catcataatg
    48841 gcgtcagcaa aaccacgcag caacatcaca atcgccacga tgatatacat gataccgagg
    48901 cgtttatggt cgacggaggt cagccactct ttccacaggt aggtccactt accgaagtaa
    48961 gtgatcaggc caacgagcgc cagacctccc aaaataatgc cagcgatcgt aaccatgacg
    49021 ataggttcat ggaacgggac tgcatcaagt gataattttc cgaacatctt tattcttcct
    49081 caaccccttt aatgggcgga ttccgcgtgg ctcatgtcca tgccttccat accttcatgt
    49141 gcgctgtgct caccttctgg ctgggtcatg tccatgctct taccgtgagc cataaacttg
    49201 ttaatcacat cggcaaacaa gtctggtttc acgttggaga aatattccac ctggttgtat
    49261 tcgctaggcg cggccagttt ttcgaacgct gccatgtcag acatgctgtt cggcgactgt
    49321 ttcgcttttg cgacccattg gtcgaatgcg gcgcgatccg gtgttgcaat agctttgaac
    49381 ttcatgcctg agaagcccgg gccgctgtag ctggcggaga taccgtcata agtaccgggt
    49441 tcgttggcga tcagatgcag gcgagtctgc ataccggcca tggcataaat ctggctaccc
    49501 agacgcggaa tgaagaagga gttcatcacg gagttggagg tcactttgaa gtacaccgga
    49561 gtgttcgccg ggaaagcgat ttcattcacg gttgcaatgc cctgttccgg gtagatgaag
    49621 aaccatttcc agtccatgga aaccacttcg atggtaatgg gcttctcgtc gtgtgccagc
    49681 ggcttgctag gctcaagagc gtgagtggtt ttccaggtca gtaccgcaag gaagatgatg
    49741 attaagatag gtaccgtcca gaccacagct tccactttat tggagtgtga ccagttcggg
    49801 ctgtacttag catctttatt gctcgcacgg tacttccagg cgaaaccaac agccatcaag
    49861 attgcgggaa taacgacaat caacatcagg ccaaatgccg tcagtatcag tgaacgttgc
    49921 tccagaccaa tctgtccttt gggatctaac agcgcagaat tacagccact gagcaataca
    49981 gtgcctgcaa ataatgacaa ccatcccaaa cttttattgt atttcctgag tctcatttaa
    50041 cgacctcaat tccacgggat ctggtggcgt ttaaagtgtg tgggcatttt acgggaaggt
    50101 tacattactg taaacatgat taaattcctg ttacttggat ttggcttaca ggtcacatat
    50161 gttgcaaaac atatcaggtt gattttaaga agctgttgca aaagggcgtt ataacaaaag
    50221 gggagtgcag aaggagtctg ggcgggcaat tggtataacc aatgcgaaat aaaacaatta
    50281 tttaacaaat aattatcatt aaggtgacaa ctgggtagtg ggaaaataaa gactcattag
    50341 ctgaatcgtg taagaaaaat agaattcata aatggcgtca ataatttcca gacgctaagc
    50401 cgcacaaaag aacaaatatt aataagcgat gctgatgttt atttagccgt aataattacg
    50461 gcgagtgatt actacggcta aataatattt acagattacg tcagatgcgt ttttcgtagc
    50521 gccagataat ccagtaaacc accaagcacg acgccagaaa gggcgactaa aaccccgact
    50581 tccagcagag caggcaggaa agagaagtgc gtcaaatcca gcgcgtccat cgtcaacagc
    50641 agtaaccaca cggccaacag gctgacgccc gccgccagtg tccacatggc aaaggcataa
    50701 cctgccggat attcggtacg ggagataaag ttatcattta ctcgtgtata ttcaagcgtc
    50761 tggcgacaaa ccagcagcaa gataagccct ggtacggcag cggcgacgga gaatagatag
    50821 aatgtcgacc agccgtgtgc ttcaacaaac caacccgcca cggggccgac ataaactcgc
    50881 cctacagcag aaagcgctga gagcagggca aattgagtag cggaaaatga cttattacac
    50941 agcgtcatta acagcgcgac aaaggctgat gtacccatcc cgccacagag gttttcgaaa
    51001 aagacggctg cgcccatgct gtagagatgc ttatcagtaa tcgacagcag ccagtaaccg
    51061 gcgttagatg caccttgtaa aatgccgaaa atcagcagtg cacggaacag cgacaggcgc
    51121 tgcatcaaaa tcccaccgta caatgcgcca acaatggtcg ccagtaagcc aagcgttttg
    51181 ttaaccacgc ctacttcacc cgcatcaaac ccgacgccgc gaatcaaaaa cgtggttgtc
    51241 aggctcatag cgaatgcgtc gcccagctta tacagcacga taagaagcaa aataagccag
    51301 gcattattgc gaccaaagaa atctcgcaga ggtgcaacaa ccgcttgttc cagcgttttg
    51361 ggcacaggaa tggtgtcggt gggttctggt gcaagcaacg tcgcgataat acaggggatc
    51421 aacagcgccg ccatcaacca gtacatgccc tgccagccca gccatttatc tgccagccac
    51481 agggctaagc cgccggaaac cagcatccct aaacggtaac ccagcacgct gattgccgcg
    51541 cccgcaccac gttcttctgc cgggagcaca tcggttttcc acgcatcgaa gacaatatcc
    51601 tgggaggcag agcaaaaagc gatcaccacc gccagtgccg ccatccagcg gagctgggtg
    51661 cctggttcga gaaaacccat cgccgcaatg gcgactaata acagaatttg cgtggcgagc
    51721 agccaaccgc gccgccgccc gaaaaatggg ggcgtgtagc ggtccatcag tggtgaccag
    51781 aggaatttaa aaacgtaagc ctggcctacc agagagaaga aaccaatggt tttgagatcg
    51841 atattctcga ccgtcatcca ggcctgtaag gtgccggacg tcagggcgag cggtagcccg
    51901 gaagcaaaac ccaggatcag caatatggct gaacgcggct gttgaaaaat acgtaaatat
    51961 tgactggaca tgggcgtcta caggcccggc ttgcaccggg ccagagggca gaaattaacg
    52021 cgcgttctgc ttgatgaatt cgtggatgct ggtgtcctga gacatatcag cgatggtatc
    52081 ggtcagcaca ctgttaaccg catcggcgat atttttgttg gaggcctgga acgcaccttc
    52141 aacgttgtag ctggcacgat agtttttggt cattttgttg ccattctgcg cggtagcgat
    52201 gatggcgata tccgctttgg tcgcgatgtt gtagcgcacg ttgccctggg acacgtcagc
    52261 atacagttgg ctaacgatga tttgcagatt aaccgggcca ttcggaccaa ccatgtatcc
    52321 acgggcggtc atttgttttt ccagcacttc ttgcagcagg aaacgcagat cgcgggaggc
    52381 ggtcagggta acgatttgat tatcgcgggt gacttttgcc agcgcctgat cggtacgctg
    52441 atcggcacca ttaatgctta cggtgacgcc catcaggctt ggatcctgct gtggcagtgt
    52501 aatcgtcggg gaaacttcaa tagttgttgg cggttttgcg catcctgcaa gcataaacag
    52561 agcaactaac gggaagagga tttttttgaa catgttcggg ctctcagaga ctcttaagcg
    52621 tgtttggtaa aaattcccgc catcataaca ttgccaacgg cgaggggaag tgggtaaggc
    52681 atgtaaattc atcatgttga cgaaataatc gcccctggta aaagaaacgc tgatgcgagg
    52741 cctgtgtttc aatctttaaa tcagtaaact tcatacgctt gacagaaaaa ccaggacgaa
    52801 acctaaatat ttgttgttaa gctgcaatgg aaacggtaaa agcggctagt atttaaaggg
    52861 atggatgaca tctcagcgtt gtcggaggag atatttcatg atgatacgtg agcggataga
    52921 agaaaaatta agggcggcgt tccaacccgt attcctcgaa gtagtggatg aaagctatcg
    52981 tcacaatgtc ccagccggct ctgaaagcca ttttaaagtt gtgctggtca gcgatcgttt
    53041 tacgggtgaa cgttttctga atcgtcatcg aatgatttac agtactttag cggaggaact
    53101 ctctactacc gttcatgcgc tggctctgca tacttacact attaaggagt gggaagggtt
    53161 gcaggacacc gtctttgcct ctcctccctg tcgtggagca ggaagcatcg cgtaaaaacg
    53221 catttgcaac tgtcggcgct tttccagtat gttgctaaag attttatgaa aaacggcctg
    53281 cgggccgttt tgttttgtct ggattttgcg ctttttgccc agcattcaga cgaaaattgc
    53341 ccgggaattg tgaaaaaata cgcgacagcg cgcaataacc gttctcgact cataaaagtg
    53401 atgccgctat aatgccgcgt cctatttgaa tgctttcggg atgattctgg taacagggaa
    53461 tgtgattgat tataagaaca tcccggttcc gcgaagccaa caacctgtgc ttgcggggta
    53521 agagttgacc gagcactgtg attttttgag gtaacaagat gcaagtttca gttgaaacca
    53581 ctcaaggcct tggccgccgt gtaacgatta ctatcgctgc tgacagcatc gagaccgctg
    53641 ttaaaagcga gctggtcaac gttgcgaaaa aagtacgtat tgacggcttc cgcaaaggca
    53701 aagtgccaat gaatatcgtt gctcagcgtt atggtgcgtc tgtacgccag gacgttctgg
    53761 gtgacctgat gagccgtaac ttcattgacg ccatcattaa agaaaaaatc aatccggctg
    53821 gcgcaccgac ttatgttccg ggcgaataca agctgggtga agacttcacc tactctgtag
    53881 agtttgaagt ttatccggaa gttgaactgc agggtctgga agcgatcgaa gttgaaaaac
    53941 cgatcgttga agtgaccgac gctgacgttg acggcatgct ggatactctg cgtaaacagc
    54001 aggcgacctg gaaagaaaaa gacggcgctg ttgaagcaga agaccgcgta accatcgact
    54061 tcaccggttc tgtagacggc gaagagttcg aaggcggtaa agcgtctgat ttcgtactgg
    54121 cgatgggcca ggggcgtatg attccgggct ttgaagacgg tatcaaaggc cacaaagctg
    54181 gcgaagagtt caccatcgac gtgaccttcc cggaagaata ccacgcagaa aacctgaaag
    54241 gtaaagcagc gaaattcgct atcaacctga agaaagttga agagcgtgaa ctgccggaac
    54301 tgactgcaga attcatcaaa cgtttcggcg ttgaagatgg ttccgtagaa ggtctgcgcg
    54361 ctgaagtgcg taaaaacatg gagcgcgagc tgaagagcgc catccgtaac cgcgttaagt
    54421 ctcaggcgat cgaaggtctg gtaaaagcta acgacatcga cgtaccggct gcgctgatcg
    54481 acagcgaaat cgacgttctg cgtcgccagg ctgcacagcg tttcggtggc aacgaaaaac
    54541 aagctctgga actgccgcgt gaactgtttg aagaacaggc taaacgtcgc gtagttgttg
    54601 gcctgctgct gggcgaagtt atccgcacca acgagctgaa agctgacgaa gagcgcgtga
    54661 aaggcctgat cgaagagatg gcttctgcgt acgaagatcc gaaagaagtt atcgagttct
    54721 acagcaaaaa caaagaactg atggacaaca tgcgcaatgt tgctctggaa gaacaggctg
    54781 ttgaagctgt actggcgaaa gcgaaagtga ctgaaaaaga aaccactttc aacgagctga
    54841 tgaaccagca ggcgtaattt acgcagcata acgcgctaaa ttcgcacaaa ggcccgtcac
    54901 cgccaggtgg tgggcttttt tttgtcatga attttgcatg gaaccgtgcg aaaagcctct
    54961 ttcggtgtta gcgtaacaac aaaagattgt tatgcttgaa atatggtgat gccgtaccca
    55021 taacacaggg actagctgat aatccgtcca taaggttaca atcggtacag caggtttttt
    55081 caattttatc caggagacgg aaatgtcata cagcggcgaa cgagataact ttgcacccca
    55141 tatggcgctg gtgccgatgg tcattgaaca gacctcacgt ggtgagcgct cttttgatat
    55201 ctattctcgt ctacttaagg aacgcgtcat ttttctgact ggccaggttg aagaccacat
    55261 ggctaacctg attgtggcgc agatgctgtt cctggaagca gaaaacccag aaaaagatat
    55321 ctatctgtac attaactccc caggcggggt gatcactgcc gggatgtcta tctatgacac
    55381 catgcagttt atcaagcctg atgtcagcac catctgtatg ggccaggcgg cttcgatggg
    55441 cgctttcttg ctgactgcag gggcaaaagg taaacgtttc tgcctgccga attcgcgcgt
    55501 gatgattcac cagccgttgg gcggctacca gggccaggcg accgatatcg aaattcatgc
    55561 ccgtgaaatt ctgaaagtta aagggcgcat gaatgaactt atggcgcttc atacgggtca
    55621 atcattagaa cagattgaac gtgataccga gcgcgatcgc ttcctttccg cccctgaagc
    55681 ggtggaatac ggtctggtcg attcgattct gacccatcgt aattgatgcc agaggcgcaa
    55741 ctgtgccgct atacttatcc agggcggcac aacgctgtaa gcggcttgcg cctgagaatg
    55801 gcatttgcgt cgtcgtgtgc ggcacaaaga acaaagaaga ggttttgacc catgacagat
    55861 aaacgcaaag atggctcagg caaattgctg tattgctctt tttgcggcaa aagccagcat
    55921 gaagtgcgca agctgattgc cggtccatcc gtgtatatct gcgacgaatg tgttgattta
    55981 tgtaacgaca tcattcgcga agagattaaa gaagttgcac cgcatcgtga acgcagtgcg
    56041 ctaccgacgc cgcatgaaat tcgcaaccac ctggacgatt acgttatcgg ccaggaacag
    56101 gcgaaaaaag tgctggcggt cgcggtatac aaccactaca aacgtctgcg caacggcgat
    56161 accagcaatg gtgtcgagtt gggcaaaagt aacattctgc tgatcggtcc gaccggttcc
    56221 ggtaaaacgc tgctggctga aacgctggcg cgcctgctgg acgtcccgtt caccatggcc
    56281 gacgcaacca cgctgaccga agccggttat gtgggcgaag acgttgaaaa catcattcag
    56341 aagctgttgc agaagtgcga ttacgacgta cagaaagcgc agcgcgggat tgtctacatc
    56401 gatgaaatcg acaagatttc tcgtaagtca gacaacccgt ccattacccg agacgtttcc
    56461 ggtgaaggcg tacagcaggc actgttgaaa ctgatcgaag gtacggtagc tgctgttcca
    56521 ccgcaaggtg ggcgtaaaca tccgcagcag gaattcttgc aggttgatac ctctaagatc
    56581 ctgtttattt gtggcggtgc gtttgccggt ctggataaag tgatttccca tcgtgtagaa
    56641 accggctccg gcattggttt tggcgcgacg gtaaaagcga agtccgacaa agcaagcgaa
    56701 ggcgagctgc tggcgcaggt tgaaccggaa gatctgatca agtttggtct tatccctgag
    56761 tttattggtc gtctgccggt tgtcgcaacg ttgaatgaac tgagcgaaga agctctgatt
    56821 cagatcctca aagagccgaa aaacgccctg accaagcagt atcaggcgct gtttaatctg
    56881 gaaggcgtgg atctggaatt ccgtgacgag gcgctggatg ctatcgctaa gaaagcgatg
    56941 gcgcgtaaaa ccggtgcccg tggcctgcgt tccatcgtag aagccgcact gctcgatacc
    57001 atgtacgatc tgccgtccat ggaagacgtc gaaaaagtgg ttatcgacga atcggtaatt
    57061 gatggtcaaa gcaagccgtt gctgatttat ggcaagccgg aagcgcaaca ggcatctggt
    57121 gaataattaa ccattcccat acaattagtt aaccaaaaag gggggatttt atctcccctt
    57181 taatttttcc tctattctcg gcgttgaatg tgggggaaac atccccatat actgacgtac
    57241 atgttaatag atggcgtgaa gcacagtcgt gtcatctgat tacctggcgg aaattaaact
    57301 aagagagagc tctatgaatc ctgagcgttc tgaacgcatt gaaatccccg tattgccgct
    57361 gcgcgatgtg gtggtttatc cgcacatggt catcccctta tttgtcgggc gggaaaaatc
    57421 tatccgttgt ctggaagcgg cgatggacca tgataaaaaa attatgctgg tcgcgcagaa
    57481 agaagcttca acggatgagc cgggtgtaaa cgatcttttc accgtcggga ccgtggcctc
    57541 tatattgcag atgctgaaac tgcctgacgg caccgtcaaa gtgctggtcg aggggttaca
    57601 gcgcgcgcgt atttctgcgc tctctgacaa tggcgaacac ttttctgcga aggcggagta
    57661 tctggagtcg ccgaccattg atgagcggga acaggaagtg ctggtgcgta ctgcaatcag
    57721 ccagttcgaa ggctacatca agctgaacaa aaaaatccca ccagaagtgc tgacgtcgct
    57781 gaatagcatc gacgacccgg cgcgtctggc ggataccatt gctgcacata tgccgctgaa
    57841 actggctgac aaacagtccg ttctggagat gtccgacgtt aacgaacgtc tggaatatct
    57901 gatggcaatg atggaatcgg aaatcgatct gctgcaggtt gagaaacgca ttcgcaaccg
    57961 cgttaaaaag cagatggaga aatcccagcg tgagtactat ctgaacgagc aaatgaaagc
    58021 tattcagaaa gaactcggtg aaatggacga cgcgccggac gaaaacgaag ccctgaagcg
    58081 caaaattgac gcggcgaaaa tgccgaaaga ggcaaaagag aaagcggaag cagagttgca
    58141 gaagctgaaa atgatgtctc cgatgtcggc agaagcgacc gtagtgcgtg gttatatcga
    58201 ctggatggta caggtgccgt ggaatgcgcg cagcaaggtc aaaaaagacc tgcgtcaggc
    58261 gcaggaaatc cttgataccg accattatgg tctggagcgc gtgaaagatc gcatccttga
    58321 gtatcttgcg gttcaaagcc gtgtcaacaa aatcaaggga ccgatcctct gcctggtagg
    58381 gccgccgggg gtaggtaaaa cctctcttgg tcagtccatt gccaaagcca ccgggcgtaa
    58441 atatgtccgt atggcgctgg gcggcgtgcg tgatgaagcg gaaatccgtg gtcaccgccg
    58501 tacttacatc ggttctatgc cgggtaaact gatccagaaa atggcgaaag tgggcgtgaa
    58561 aaacccgctg ttcctgctcg atgagatcga caaaatgtct tctgacatgc gtggcgatcc
    58621 ggcctccgca ctgcttgaag tgctggatcc agagcagaac gtagcgttca gcgaccacta
    58681 cctggaagtg gattacgacc ttagcgacgt gatgtttgtc gcgacgtcga actccatgaa
    58741 cattccggca ccgctgctgg atcgtatgga agtgattcgc ctctccggtt ataccgaaga
    58801 tgaaaaactg aacatcgcca aacgtcacct gctgccgaag cagattgaac gtaatgcact
    58861 gaaaaaaggt gagctgaccg tcgacgatag cgccattatc ggcattattc gttactacac
    58921 ccgtgaggcg ggcgtgcgtg gtctggagcg tgaaatctcc aaactgtgtc gcaaagcggt
    58981 taagcagtta ctgctcgata agtcattaaa acatatcgaa attaacggcg ataacctgca
    59041 tgactatctc ggtgttcagc gcttcgacta tggtcgcgcg gataacgaaa accgtgtcgg
    59101 tcaggtaact ggtctggcgt ggacggaagt gggcggtgac ttgctgacca ttgaaaccgc
    59161 gtgcgttccg ggtaaaggca aactgaccta taccggatcg cttggcgaag tgatgcagga
    59221 gtccattcag gcggcgttaa cggtggttcg tgcgcgtgcg gaaaaactgg ggatcaaccc
    59281 tgatttttat gaaaaacgcg acatccacgt ccacgtaccg gaaggtgcga cgccgaaaga
    59341 tggtccgagt gccggtattg ctatgtgcac cgcgctggtt tcttgcctga ccggtaaccc
    59401 ggttcgtgct gatgtggcaa tgaccggtga gatcactctg cgtggtcagg tactgcctat
    59461 cggtggtttg aaagaaaaac tactggcagc gcatcgcggc gggatcaaaa cagtgttaat
    59521 tccgttcgaa aataaacgcg atctggaaga gattcctgac aacgtaattg ccgatctgga
    59581 tattcatcct gtgaaacgca ttgaggaagt tctgactctg gcgctgcaaa atgaaccgtc
    59641 tggcatgcag gttgtgactg caaaatagtg acctcgcgca aaatgcacta ataaaaacag
    59701 ggctggcagg ctaattcgga cttgccagcc tttttttgtc tcgctaagtt agatggcgga
    59761 ttgggcttgc ccttattaag gggtgttgta aggggatggc tggcctgata taactgctgc
    59821 gcgttcgtac cttgaaggat tcaagtgcga tataaattat aaagaggaag agaagagtga
    59881 ataaatctca attgatcgac aagattgctg caggggctga tatctctaaa gctgcggctg
    59941 gccgtgcgtt agatgctatt attgcttccg taactgaatc tctgaaagaa ggggatgatg
    60001 tagcactggt aggttttggt acttttgccg ttaaagagcg tgctgcccgt actggccgca
    60061 atccgcagac cggtaaagag atcaccatcg ctgctgctaa agtaccgagc ttccgtgcag
    60121 gtaaagcact gaaagacgcg gtaaactaag cgttgtcccc agtggggatg tgacgaagtt
    60181 caagggcgca tctactgatg tgcctttttt atttgtattc ggtgactttc tgcgtcttgt
    60241 gggctgacaa ttgcccccgt ttcttgtcac aatatgcctt tgcgcgcatc gatacgttgc
    60301 gtgaggtaca cagtcatcta cagcggagtg ttgttacacc atgatggaca gcttacgcac
    60361 ggctgcaaac agtctcgtgc tcaagattat tttcggtatc attatcgtgt cgttcatatt
    60421 gaccggcgtg agtggttacc tgattggcgg aggcaataac tacgccgcaa aagtgaatga
    60481 ccaggaaatc agtcgtgggc aattcgagaa cgccttcaac agcgagcgta atcgcatgca
    60541 gcaacagctg ggcgatcaat actccgagct ggcagcgaac gaaggctata tgaaaaccct
    60601 gcgtcaacag gtgctgaatc gtctgatcga cgaggcgctg ctggatcagt acgctcgtga
    60661 gctgaaactg ggtatcagcg atgagcaggt taaacaggcg attttcgcga ccccagcctt
    60721 ccaggttgac ggcaaatttg ataacagccg ctataacggt atcctcaacc agatggggat
    60781 gaccgccgat cagtacgccc aggcgctgcg taaccagctc actacccaac agctgattaa
    60841 cggcgttgcc ggtaccgatt ttatgctgaa aggtgaaacc gacgagctgg cggcactggt
    60901 agttcaacaa cgcgtggtgc gtgaagcgac tatcgatgtt aacgcgctgg cggcgaagca
    60961 gcctgtgacc gaacaggaaa ttgccagcta ctacgaacaa aacaaaaaca atttcatgac
    61021 gccggaacaa ttccgcgtga gttacatcaa gctggatgcc gcaacgatgc agcaaccggt
    61081 tagcgatgcg gatatccaga gctactacga tcagcatcag gatcaattca cccagccgca
    61141 gcgtacccgt tacagcatca tccagaccaa aactgaagat gaagcgaaag cggtacttga
    61201 tgagctgaat aagggcggtg attttgctgc attagccaaa gaaaaatctg ccgatattat
    61261 ctctgcccgc aacggcggtg atatgggttg gttagaagat gccactatcc cggacgaact
    61321 gaaaaatgct ggtctgaaaa aaaaaggcca actgtctggt gttatcaaat cttcggtcgg
    61381 tttcctgatt gtgcgtctgg acgacattca gccagcgaaa gtgaaatcgt tagacgaagt
    61441 acgtgacgac attgcggcga aagtgaaaca cgaaaaagcc ctcgatgcgt actacgcgct
    61501 gcagcagaaa gtgagcgatg cggcaagcaa cgacaccgag tctctggccg gtgcagagca
    61561 agctgccggc gttaaagcca ctcagacggg ttggttcagc aaagataacc tgccggaaga
    61621 gttgaacttc aagccagttg ccgacgctat cttcaacggc ggtctggtcg gtgaaaacgg
    61681 cgcgccgggc atcaactctg acatcatcac cgtagacggc gaccgcgcat tcgtgctgcg
    61741 catcagcgag cacaaaccgg aagcggtgaa accgttggca gatgttcagg aacaagttaa
    61801 ggcactggtt cagcacaaca aagctgaaca acaggcgaaa gtggatgctg agaaactgct
    61861 ggttgatttg aaagcaggca aaggtgcgga agctatgcag gctgccggtc tgaaatttgg
    61921 cgagccgaaa accttaagtc gttccggtcg tgacccgatt agccaggctg cgtttgcact
    61981 gccgctgcca gcgaaagaca aaccgagcta cggtatggcg accgatatgc aaggtaatgt
    62041 agttctgctg gcgctggatg aagtgaagca aggttcaatg ccggaagatc agaaaaaagc
    62101 gatggtgcag ggtatcaccc agaacaacgc acaaatcgtc tttgaagctc tgatgagtaa
    62161 cctgcgtaaa gaggcgaaaa tcaaaattgg cgatgcgctg gaacagcaat aatcctgaag
    62221 ccgcctcgca aaaaaatgct ttgcagctgt aacaagaaaa ggtcgctttc gcggcctttt
    62281 ccatttctga acattgccat ttgtttactg ttttcactgc cgttaaggtg attccactgt
    62341 taacaaacaa ggagaaaaca gtatgaaaca cggaattaaa gcactgctca ttaccctgtc
    62401 cctggcctgt gccggaatgt ctcatagtgc tctggcggca gctcctgcgg caaaaccgac
    62461 aacggtagaa accaaagcgg aagctcctgc agcacaaagt aaagcagcag ttccggcgaa
    62521 agccagtgac gaagaaggca cccgggtcag cattaataat gccagcgcgg aagagttagc
    62581 ccgcgcgatg aatggcgttg gcctgaagaa agcgcaggcg attgtcagtt atcgcgaaga
    62641 gtacggtccg tttaaaacgg tggaggatct aaagcaggtg ccggggatgg gcaattcgct
    62701 ggtggagcgt aatctggcgg tattaaccct gtaattaatt tgcatagtgg caattttttg
    62761 ccagactgaa gaggtcatac cagttatgac ctctgtactt ataacaacaa cgtaaggttc
    62821 tcgcgctatg caaacacaaa tcaaagttcg tggatatcat ctcgatgttt accagcacgt
    62881 taataacgcc cgctatctgg agtttctcga agaagcccgc tgggatgggt tggaaaatag
    62941 cgacagtttt cagtggatga cggcccataa catcgctttc gtggtggtga atatcaatat
    63001 taactatcgt cgcccggcgg tattaagcga cttgttaacc attacgagcc agttacagca
    63061 attaaacggt aaaagcggca tcttaagcca ggtcattaca ctggagccgg aagggcaggt
    63121 ggtagcggat gcgcttatta cgtttgtttg tattgatctt aaaacgcaga aagcattagc
    63181 tctggaaggg gaattgcgcg aaaagctcga gcagatggtt aagtaaacgt tttgtggtgc
    63241 cggatgctca agccgcatcc ggcgacaccc ggaataatta cttcaacccg gttttctgct
    63301 tcatcgctgc catcaccgtc ggtttatcgg ccagataatg atgcaaaccg ttggcacgta
    63361 aattacacgc cgcacaatga ccgcaaccgt cgcctttaat gccgttataa caggtcagcg
    63421 tttcgttacg gactaaatcc agcttgccgt aataatctgc cagcgcccag gtttccgctt
    63481 tatcaatcca catcagcggc gtttcaaaac gaatgtcttt cgccataccc agactgacgg
    63541 catggttcag tgctttcaca aactcatcgc ggcaatccgg gtagccggag aaatccgttt
    63601 cgcagacgcc ggtaatcacc gcttctgctt ttacctgata cgcatatatt gccgccagtg
    63661 tcaggaacaa aatattacgc ccagggacaa acgtattcgg gatgccatcg gcttcaggtt
    63721 cataatctgg cactggaatg ctgtcacgtg tcaggctgct gaccgccagc tcgttgagca
    63781 gcgttacatc aagaacctta tgcgcgcgtg cccccagttt cagcgccagt tcgcgtgcca
    63841 cgtcgatttc cgcgcgatgt cgctgaccgt aatcgaacgt cacgcaatgg acttcatcat
    63901 attgttgtaa tgcctgcacc agacaggtgg tagaatcctg gcctccactg aacacaacga
    63961 cagcacgttt catagataat cctgcttgaa caataaaagc gttatggtaa cgcctgcgat
    64021 gaacccggac cagctattca ttgcgatggc gcgggaagcc aggcttcggt aaattcaaac
    64081 cagcctcgcg gtgtcagtcg cacaccgttc acgccgggag gggcgctaat gcgatagtga
    64141 tagttgaaca gcggcgtcag cgtcgcatct gccattaact ggctaaaaac cgctttcagg
    64201 gcattaaatc ggttttcctc atcaggcatt acttgcaccg cgtccagtgt cgattgcaaa
    64261 tgtgcatatg ctggagcgtc gaaaacatgt ggccatagtg gatcgcaacg cagccattgc
    64321 tccagggtat attccggtgc ttcgccaatt aatctgtcgc ccatcatgag gtctgcgtgt
    64381 gccagtaggg tcgtgtcgtc ccagtttttt gcgttatgaa aaataattgt gagttcacag
    64441 ccttccgctg ccagtgtcgc ctgtagacgt tctgccatgg tatgaagttc tatcggtagg
    64501 tgataaacca gcgtcaaggt tttcggtagt ttgacctcat ccggtacttg ccattgcgga
    64561 atagtccagc ctggcagtaa tgcatgactg gcggtgatca ggttttctcc gacttctaac
    64621 gtttgtaata aaccggattg atgaataatg gaaatcactt ttcgcgcctg ccagagggac
    64681 agtcggggac ttttgcgcaa cgttaaatag caaaaaccta aactgatgcc actactgacc
    64741 tggctgaccc gttgcagctc ctccggtttg ccgatggtga tttgcacggg atgccgacaa
    64801 ctggttccca aatctttttc gaaaagcggc ggagttatcc agtactcaac cgctttaagc
    64861 agcggatgac gtaaatggta ataatcatgg ctttccaggc gcaccagctc cgctgtgaat
    64921 tgtgttaagc gaaaaggacc cgtgccgatc agtgggaatt gcggatgcgc cagatggctg
    64981 caatagctcg ccagtcggtg cgcaagccag taatcggggc gatgtaaaaa gaaggtcaga
    65041 cactgcggat gggtgacttc aatacgcttc acgctaataa ataattgatc ccgtgctggc
    65101 agttgtaaca gcagcaataa tcgctggtgt aagtgtgagg cttttactgc atcgccgtta
    65161 tgccagtgta gggttgaacg aagataaaag tcccagcgta acccgtcagt agaggtttcc
    65221 cagtgatgtg ctaaatcgcc aatcgggcgc tgagtattat tatcgaagcg ggtcaggccg
    65281 gaaaatatct gcccggccag atgctgctcg gcacggccgg gcaaaaagcc cggttgtagc
    65341 ggttcgagcg ggcgatagta gggaatacgc aacgtgggcg tatcgttttg ccattgtccg
    65401 cccataaacg gctgtaacag agtgcgcagc tcacctgggg ccagttgcgc cagctccagc
    65461 acatcttgct gctttccggt ttccagcgcc tgttccatca tcgcattgcg tagcgattct
    65521 ggcgtgacca gaaagcgtag ttgcccgcgt tttccgcgtc ctgactgcgc ctgccactcc
    65581 agccatcccg cctcctgtgc ctggcgtaac agcgtacgaa catggcgttc gctgcaaaaa
    65641 cagcgttcgg ccagttcgct gacggtgacg gtttgcggct ttccggcgga aggttgccac
    65701 agacgttgat actggttaag acggttgagc agtcgcatat aaacccggaa caatattatt
    65761 taactattca ctattacttc cgtatatatc aggtgatact caatcaccat taaccgtgtc
    65821 acagagtgga gaaagaaatg gctcgtctgg cagcatttga tatggatggc actttattga
    65881 tgcctgacca tcatttaggt gagaaaaccc tctctacttt ggcgcgactg cgtgaacgcg
    65941 acattaccct cacttttgcc actgggcgtc atgcgctgga gatgcagcat attctcgggg
    66001 cgctatcgct ggatgcatat ttgattaccg gcaacggaac gcgcgtgcat tctctggaag
    66061 gtgaactttt acatcgtgat gatttacctg cggatgtcgc ggagctggtg ctgtatcagc
    66121 agtgggatac ccgagccagc atgcatatct tcaatgacga cggttggttt accgggaaag
    66181 agatccctgc gttgttgcag gcatttgtct atagtggttt tcgttatcag ataatcgatg
    66241 tcaaaaaaat gccactcggc agcgtcacca agatctgctt ctgtggcgat cacgacgatc
    66301 ttacacgctt gcagatccag ctatacgaag cattaggcga gcgtgcacat ttgtgttttt
    66361 ccgccacgga ttgcctcgaa gtgctgccgg tgggctgcaa taaaggcgct gcattgacgg
    66421 tgctgaccca acatttaggt ttatcgttgc gcgattgcat ggcctttggt gatgcgatga
    66481 acgatcgcga aatgttaggc aacgtcggta gcggatttat tatgggcaat gcgatgccgc
    66541 aattgcgcgc ggagctcccg catttaccgg tgattggaca ttgccgaaat caggctgtct
    66601 ctcactattt gacgcactgg ctggactatc cacatctacc ttattccccc gaataacgag
    66661 atcccttcca gcaccgggca attgcccggt tttttttgcg ttgaatttgt cattttgtgc
    66721 cgtggtgttt aaaccgcaca gaataaattg tcgcgatttc acctttaaaa tagaattaaa
    66781 agagaaaaaa attctctgtg gaagggctat gttagataaa attgaccgta agctgctggc
    66841 cttactacag caggattgta ccctctcttt gcaggcactg gctgaagccg ttaatctgac
    66901 aaccaccccg tgctggaagc gtctgaaacg gctggaggac gacggtattc ttatcggcaa
    66961 agtcgccctg ctggatccgg aaaaaatagg cctcggcctg accgcctttg tgctgataaa
    67021 aacgcaacat cacagcagcg aatggtattg ccgctttgtc acggtggtta ccgaaatgcc
    67081 agaagtgctg gggttctggc gcatggccgg tgaatacgat tatctgatgc gcgtccaggt
    67141 tgccgacatg aaacgctacg acgagtttta taagcgtttg gtaaacagcg tgccggggct
    67201 gtcggacgtc acttccagct tcgcgatgga acagattaaa tacaccactt ctttacccat
    67261 cgaataaata tccagaatca ggtcaggaca caacgcgtgc gattatttgc tcaattaagc
    67321 tggtatttcc gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct tgtcattatc
    67381 gcgatgctgc aactggttcc gccaaaagtg gttggtattg ttgttgatgg cgtgacagaa
    67441 caacacttta ctaccgggca gatcctgatg tggatcgcca ccatggtgct gattgccgtt
    67501 gtggtttatc tcctgcgtta cgtctggcgt gtattgctgt ttggcgcgtc ttatcaactg
    67561 gctgttgaac tgcgtgaaga ttattaccgt cagctaagcc ggcagcatcc tgagttttac
    67621 ctgcgtcatc gcacgggtga cctcatggct cgtgcgacca atgacgtcga tcgcgtcgtg
    67681 tttgccgccg gagaaggggt gctgacgctg gtggattcac tggtaatggg ctgcgctgtg
    67741 ttgattatga tgtctacgca aattagctgg cagttgacct tatttgccct gttgccgatg
    67801 ccagtgatgg cgatcatgat taagcgcaac ggcgatgcct tgcatgaacg ctttaagctg
    67861 gcgcaggcgg cgttttccag tcttaatgac cgcacccagg aaagcctcac cagtatccgc
    67921 atgatcaaag cttttggtct ggaagatcgc cagtcggcgt tatttgccga ggatgccgaa
    67981 gataccggca aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga cccgaccatc
    68041 tatatcgcga ttggtatggc gaacttgctg gcgattggcg gcggtagttg gatggtggtg
    68101 cagggcagtt taacgctggg ccagctcacc agttttatga tgtatttagg tctgatgatt
    68161 tggccaatgc tggcgctggc atggatgttt aacatagtgg aacgtggtag tgctgcgtac
    68221 agccgcattc gcgcgatgct ggcggaagcg ccggtggtga atgatggtag cgaacccgtg
    68281 ccggaaggtc gtggcgaact ggatgtaaat attcgccagt tcacgtatcc gcagactgac
    68341 catcctgcgc tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct gggtatttgc
    68401 gggccgactg gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca tttcgacgtc
    68461 agcgaggggg atatccgctt tcatgatatt cctctgacga agttacaact cgatagctgg
    68521 cgtagccgcc tggcggtagt tagccagaca ccgttccttt tttccgacac cgtagcgaac
    68581 aacatcgcgt tgggttgccc gaatgccacc cagcaagaga ttgagcatgt cgcgcggtta
    68641 gccagcgtgc atgacgatat tttgcgttta ccgcaaggtt acgatacaga agtgggcgag
    68701 cgcggcgtga tgctttccgg tgggcaaaaa cagcgtatct ccattgctcg tgcgttatta
    68761 gtcaacgcgg aaatcctcat ccttgatgat gcgctttcgg cagtagacgg acgcacagag
    68821 caccagatcc tgcataacct gcgtcagtgg gggcagggaa gaacggtaat catcagcgcc
    68881 catcgccttt ctgcactgac ggaagccagt gaaattattg tgatgcagca cggacatatc
    68941 gctcagcgtg gcaatcatga tgagctggcg caacaaagcg gctggtatcg cgatatgtat
    69001 cgctatcaac aactggaggc ggcgctcgac gacgctccgg aaattcgcga ggaggccatc
    69061 gatgcgtagt tttagccaac tgtggccgac tctcaagcgt ctgttagcgt acggttcgcc
    69121 gtggcgtaaa ccgctgggga ttgcggtcat aatgatgtgg gttgcggcag cggcagaagt
    69181 cagtgggccg ctgcttatca gctattttat cgacaatatg gtagcgaaaa ataacctgcc
    69241 gttgaaagtg gttgcagggc tggctgcggc gtatgttggg ctgcaactgt ttgccgccgg
    69301 gctacattac gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag tgcaacagtt
    69361 gcgtaccgac gtgatggatg ctgcgttacg ccagccatta agcgagtttg atacccaacc
    69421 cgtcgggcag gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg acctctacgt
    69481 taccgtagtg gcaactgtcc tgcgtagcgc cgcactggtg ggcgcgatgc tggtggcgat
    69541 gttcagcctc gactggcgaa tggcgctggt ggcgataatg attttcccgg tggtgctggt
    69601 ggtaatggtg atatatcagc gttacagcac gccgattgtc cgtcgtgtgc gcgcttatct
    69661 ggcagatatc aacgacggct ttaacgaaat tatcaatggc atgagcgtta tccagcagtt
    69721 ccgtcagcag gcgcgatttg gcgaacgtat gggtgaggcc agtcgttcac actatatggc
    69781 gaggatgcaa accctgcgcc tcgacggttt tctgctgcgt ccgctgctga gtctgttttc
    69841 atcgctcatt ctttgtggct tgttgatgct gtttggcttc tccgccagcg gcaccattga
    69901 agtgggcgtg ttgtatgcgt tcatcagtta tttggggcga cttaacgaac ccttaatcga
    69961 actgaccacg cagcaggcga tgctgcaaca ggctgttgtt gctggtgagc gcgtttttga
    70021 gctgatggac ggaccgcgcc agcaatatgg caatgatgat cgcccgttgc agagcggcac
    70081 catcgacgtc gataacgtgt catttgctta tcgcgatgac aatctggtgc taaagaacat
    70141 taatctctct gtgccttcgc gcaactttgt ggcgctggtc gggcataccg gcagtggcaa
    70201 aagcaccctc gccagtttat tgatgggcta ttacccgcta acggaaggtg agattcgcct
    70261 tgatggtcgt ccattaagta cgctaagtca cagcgcgctg cgccagggcg tggcaatggt
    70321 gcagcaagat ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc tgggacgtga
    70381 tatctctgaa gaacgcgtct ggcaggcgct ggaaatcgtg cagctggcgg agctggcgcg
    70441 tagcatgagt gatggtattt acacgccgct tggcgagcag gggaataatc tctcagtcgg
    70501 gcaaaagcaa ctgctggcac tggcgcgcgt gctggtggag acgccgcaaa tcctgatcct
    70561 tgatgaggca accgccagca ttgactccgg gactgaacag gcgattcaac atgctctggc
    70621 ggcggtgcgt gaacatacta cgcttgtggt gattgctcac cgcttatcga ctattgttga
    70681 tgccaacacc attctggtgc ttcatcgtgg gcaagccgtg gagcagggca ctcaccagca
    70741 actgctggcg gcccagggac gatactggca gatgtatcaa ctgcaacttg cgggcgaaga
    70801 gctggcagcc agcgtgcgtg aagaggaatc attgagcgcc tgaatagcgc aatatttcat
    70861 cgttggtgca aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat tttcaatctt
    70921 cttaacttcc tgctctcttt ctcgtttttc atttttggca caccgcttgc aataccttct
    70981 tcgtgtagca gaaccattac cgaattctga ccggagggga tctatgaagc tggtgaccgt
    71041 gattatcaaa ccattcaaac ttgaagacgt tcgtgaagcg ttatcttcca ttggtattca
    71101 gggcctgacc gtcaccgaag tgaaaggttt cgggcgtcag aaaggacatg ccgagctgta
    71161 ccggggggcg gaatacagcg tcaatttcct gccaaaagta aaaattgatg tggcgattgc
    71221 tgatgaccaa ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca ccggaaaaat
    71281 tggcgacggc aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc gtaccggcga
    71341 agccgacgaa gcggcgctgt aatctctggc acacagcaac aggaacgaaa aatgaagata
    71401 gcgacgataa aaactgggct tgcttcactg gcgatgctgc cgggactggt aatggctgca
    71461 cctgcggtgg ccgataaagc cgacaatgcg tttatgatga tttgtactgc gctggtgctg
    71521 tttatgacta ttccggggat tgccctgttt tacggtgggt tgattcgcgg caaaaacgtg
    71581 ctgtcgatgc tgacgcaggt gacggtgacg tttgcactgg tctgtattct ctgggtggtt
    71641 tacggttact cgctggcgtt tggtgaggga aacaacttct tcggcaacat taacgggttg
    71701 atgctgaaaa acatcgaact gacggcggtg atgggcagca tttatcagta tatccacgta
    71761 gcgtttcagg gatcgtttgc ctgcattacc gtaggcttga tagttggagc actggcggaa
    71821 cgaatccgct tctcagctgt gttgattttc gtggtggtat ggctgacgct ctcctacatt
    71881 ccgattgcgc atatggtctg gggcggcggt ttgctggctt ctcacggtgc actggatttc
    71941 gcgggtggca cggtggtgca cattaacgcc gcaatcgccg gtctggtggg cgcgtatctg
    72001 ataggcaaac gcgtgggctt cggcaaagag gcgtttaaac cgcacaacct gccgatggtc
    72061 ttcaccggga ctgccattct ctatatcggt tggtttggct ttaacgccgg gtcagcgggc
    72121 acggcgaatg aaatcgcagc actggcattt gtgaatactg tggtcgcaac ggcggcggca
    72181 attcttggct ggatcttcgg tgaatgggcg ctgcgtggta agccttcact gctgggagcg
    72241 tgttcaggcg cgattgccgg tctggtcggc gtgacgccag cgtgtggcta cattggtgtt
    72301 ggcggcgcgt tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg cgttaccatg
    72361 ctcaaacgct tgttgcgggt ggatgatccc tgcgatgtct tcggtgtgca cggcgtttgt
    72421 ggcattgtcg gctgtatcat gaccgggatt tttgctgcca gctcgctggg cggcgtgggc
    72481 ttcgctgaag gtgtgacgat gggccatcag ttgctggtac agctggaaag catcgccatt
    72541 acgatcgtct ggtccggtgt ggtggcattt attggctaca aactggcgga tctgacggtt
    72601 ggtctgcgtg tgccggaaga acaagagcga gaagggctgg acgtcaacag ccacggcgag
    72661 aatgcctata acgcgtaaca agcactgcaa aaaacagccg gacggttttc acctccggct
    72721 atttttttaa ttatgattac gcattacccc ttcctgaacg gtcgaggcaa ccagcacgcc
    72781 gtcctgagta taaaactcac cgcgcacaaa gccgcgtgcg ctggacgccg aggtgctctc
    72841 cacgctatac agcagccatt cattcaaatt aaacgggcga tggaaccaca tggaatggtc
    72901 gatggtcgct atctgaattc ccggttcgag aaaaccgatg ccgtgcggct gtagagctac
    72961 cggcaggaag ttaagatcag aagcgtaacc gagcagatac tgatgaacgc gcagatcatc
    73021 cggcacgcta ccatttgcgc ggatccacac ctgacgatgc ggttctgcga cgtgaccttt
    73081 caatgggtta tgaaattcca ccggacggac ttccagcgga cgatcgcaga tgaatttatc
    73141 tttcagcact ggcggcagca ggtgcgccag cgattgggcg atttgtgttt ccgaaggcag
    73201 gccatcaggc gctggcgcgg acggcattgt tttttgatgt tcgaaacccg cttctggtgc
    73261 ctggaaagag gccgtcatat aaaaaatcgg tttgccgttt tgaatagcag caacccggcg
    73321 ggcgctgaag ctgttaccgt cacgcagcgt ttcgacatca taaataatcg gcttcttact
    73381 atcgcctggg cgaagaaagt agctgtgaaa cgaatgtacc agccgctctt cagggacggt
    73441 ctcttttgca gcatacaagg cctgacccac gacctggccg ccaaacacct ggcgtaaacc
    73501 taaatcttca ctctggccgc gaaagagtcc ttcctcaatt ttttccagat ttaacaatgt
    73561 cagtaaattt tttagcgcct gactcatata actctccagt aacaaagctg ccgcagcaag
    73621 ccaaagtgag ttgagtataa cgcaaatttg ccactggtcc gatgggtgca atggtctgaa
    73681 ttacgggcta attacaggca gaaatgcgtg atgtgtgcca cacttgttga cgttactatt
    73741 ttgttaacca ctcttccggc gaggaaagtt agcccgctgg tgcattgata ataaggagaa
    73801 gtgaatgaaa ctcgtgcaca tggccagtgg tttagcggtt gcgattgcgt tggcggcttg
    73861 cgcagataaa agcgcggata ttcagacgcc agcaccggct gcaaatacgt ctatttcagc
    73921 aacacaacct gctatccagc aaccgaatgt ctccggtacc gtctggatcc gtcagaaagt
    73981 cgcactgccg cctgatgctg tgctgaccgt gacactttct gacgcgtcgt tagccgatgc
    74041 gccgtcaaaa gtgctggcgc agaaagcggt gcgtaccgaa ggtaaacagt caccattcag
    74101 ctttgttctg ccatttaacc cggcagatgt tcagccgaac gcgcgtattc tattgagtgc
    74161 ggcgattacc gtgaatgaca aactggtatt tattaccgat accgttcagc cggtgatcaa
    74221 tcagggcgga actaaagccg acctgacatt ggtgccggtg cagcaaaccg ccgtgccggt
    74281 tcaggccagc ggtggcgcaa cggctaccgt accttcgact tcaccaactc aggtgaatcc
    74341 gtcttcggca gttccagctc ctacgcaata ttaagcgcag ttaaccctct ccgcccggag
    74401 agggttagta gttccagcga taacgctgca agtcgatttg cccgcttccc gataccatca
    74461 caccttctgc cagtaatgcc tgtcgttgac gctgtaaatc cggtccggtt agcgaaattg
    74521 tgccgtggcg attaaccacc cggtgccagg gcaaggtgct gccttcgggg agacgcttta
    74581 acacaccgcc tacctggcgc gcggcgcgcg gcgatcccgc cagtttcgcc acatcaccgt
    74641 aagtggtgac atagccttcg ggaatagcgg cgacgatttg ccagacgcgt tggggaaatg
    74701 aatcttcttt ttccatcttt tcttcctgag gtaatttttc agcataatct ggaaaaacgc
    74761 ccgagtgaag ccgcattgcg caagaaacca gcatctggca cgcaatgggt tgcaattagc
    74821 cggggcagca gtgataatgc gcctgcgcgt tggttctcaa cgctctcaat gggggctctg
    74881 ttggttctcc cgcaacgcta ctctgtttac caggtcaggt ccggaaggaa gcagccaagg
    74941 cagatgacgc gtgtgccggg atgtagctgg cagggccccc acccatttct gcctcccccc
    75001 gtttcgtcaa aaaatcccaa catggctaaa ctttaaccac aactgacgtc gcaagaattg
    75061 tctggctgcg cagtacgctt cggaggtatg tctgatgaag tatgttgatg gttttgttgt
    75121 tgccgttcct gccgataaaa aggatgccta tcgggaaatg gccgctaagg ccgcgccatt
    75181 atttaaagag ttcggtgcgc ttcgtattgt cgaatgctgg gccagcgatg taccggatgg
    75241 taaagtgacc gattttcgta tggcggtgaa agcggaagag aatgaagagg ttgtctttag
    75301 ctggattgaa tacccttcaa aagaggtccg cgacgctgct aatcaaaaga tgatgtcgga
    75361 cccacggatg aaagagttcg gcgagtccat gccgtttgac ggcaagcgaa tgatctatgg
    75421 tggattcgaa tcaatcatcg acgagtaggt agcgtgacgg gctgtgcggc gtgccgtcag
    75481 ccctaaaatt cacaaatgtt gctcggccca gcgtaagaaa tcctctttcg gtaatgcctt
    75541 gctgtagagc cagccctgac cgtagtgcac gccatgctgg cgtaaccact cttcttgttt
    75601 actggtttcg attccctccg ctaccatttt cagtttcagt gtttttgcca tttcgatgat
    75661 atgcggcgtg acatttttat attccagcgc atcaacgaaa gatttatcga tcttcagaat
    75721 gtcgacatcc agatcctgta aataacttaa acttgaatac cccgtaccaa aatcatcgag
    75781 ataaatttca tggcccgcct cccggtagcg agaaattatc ggggcgctgg ttttcggatc
    75841 ggcaaactcg cgttcagtga gttcaagcgc gatctgtctg ggattaacct gatagtgatt
    75901 gatcatttca cgcagcaatt gcgggatttt ttccgaggtg agcacggtgg attcaagatt
    75961 gatcgaaata tgctgctgcg gatgctgacg cagccagtcg cccatatctt cgaagacgct
    76021 tcttatgatc agtagcgtca acggctcaga aaggcccgtt tgctgtgcca gcggaataaa
    76081 actatctggt gacaaccaac taccgtctgt ctgcggccag cgcgccagtg cctcagcacc
    76141 gacaattttg ccattggcta aggagacaat cggctgatag tgcacgcaaa tatcacgatt
    76201 ttcgatagca tcctgcagcc gatgatgcgg tgactggata cggcgcaaaa tacgcagcac
    76261 aaacatcgct gccagcaggc caatcaccaa cccgagcggt aaccaaataa agacttgccg
    76321 atgccagcct ttctgcaaca ttttcgttga agaccaggtg atgatcgaaa tattcatttc
    76381 cgggaagggc aggatatcgt aaatgattcc attattttcg atatgctcac cgggtgtttt
    76441 ttgtagtctg gtaataattc cctgagcaat ttcatcgctg ctggttatca caacgttatg
    76501 ggcattgcca ataatggcgg catcaatttg ccatgagcta tagggaatga catcaataaa
    76561 ggaagcgggg tcgatcatga cgacataatg tgccgttccc atggcaacca tgtaacggat
    76621 aatgcctaaa tcgttatgcg atgttaacca gacacgataa ccatctttcg aaattttacc
    76681 tggctcgggg aaggtatcgg gcgggctttc atgctccaga gacgaacact ggggaacgtt
    76741 gttatcgata taaaccactt cctgaatata gcgataactg taagataccc gacgcatttc
    76801 catgagatgg gcttcgctac aggcagcgcc ttgccatctt tccagctctt gcagcgcatc
    76861 tttcccttgt gtcgccacct tattggcccg aatagcgacg cgggaggaat acgtattcag
    76921 ctcttcaata aatgatgttt ctacctgctg atgggccagc cagatgctta agccgacagg
    76981 cagcaatact gaaagaatca gtactcccga aataaggccg accagatgtc gtgttctcac
    77041 cagcatatcc ttattaacac agtgtaggga aagtatatct gattgtggaa aaaggctggc
    77101 aggagataag gaggtagagc cgggatttca tgaagggatg ggtggaaacg aacgttgtca
    77161 tttgaccagg cgcagagctt tacgcctggt atgtaaccct aacttaagat gttgattcgg
    77221 cgcgagcgag ggtaaaaata tcgtaaaaag atagttcacc tttacggacg atcatttttt
    77281 gcagttgttc tttatgaaca tccgcgacac gcggagagtt caggatggct tcaactagcg
    77341 cggcaggtac aaagcgcgtg ttgtaccatt cgccgttata gcaaacgcgg aaatccagca
    77401 cgtcgatatc ttcatagcgg tagcgtgggt agacatcgaa aaagaagaaa ccattaagca
    77461 ggataaataa tacaaccagt agccagacag agcctgaaag cgtttcggac tgcaacatca
    77521 ctgccagggt ggcaaaaaaa gcaacgtaca taccgataaa caaccccgga tgtttacgga
    77581 taaaactgat actaaagcgg ggtttgttgt cgcgtttttc gcgggtattt agagactcaa
    77641 ttgtttcggt cagcaggcgt tgtatttcgg tcatcgtttg ccttcgtggt atctgcaact
    77701 ttccagggaa caccctattt taacgtggga atagtagagg gaaagtaaca gatttaccac
    77761 aattttgcat aacagttgcg aaaaaccgat tacaattttt taattattct ggctggatta
    77821 ccgcccacga caacgttgtc cgggacatct tttgtgacaa ctgcacctga ggctaccacg
    77881 acgttatcac caatggtcac accagggtta atgaccgcac gtccgccaat ccagacgtta
    77941 ttaccgatgg tgacgggttt ccccagttca gcaccgctat tacgtgctac agggtcgatg
    78001 ggatgtgttg ccgtgtagat atgaacgcct ggtgccaaca tacagttatc accaatgcga
    78061 atagggcaga catcaagcat cacgcaatcg aagttggcga aaaaattatt gccgagaaaa
    78121 atgttataac catagtcaca gcgaaacgtt ggctcaatat aagcctctgt cacctgaccg
    78181 aataaatcag caagaatttg ctggcgtaat gtgtgctctt ccgccaggga atgattgtat
    78241 cggtgaataa gccgacgagc gcgcaggcga tcgcgagata acgcctcatc tgccgagtga
    78301 tacaactcac cagcaatcat cttttctttt tctgtgctca ttactgacct ccggttgctg
    78361 aaaatgcggc aacacagtaa gacgaaaagt cagtgaagag aacgttccca ttacatctgt
    78421 gtgatgaaga tcacataggg tatgccttca tggcgaaaga attataaaac tcttaataaa
    78481 cagccggtta tagctccgaa agcgagttat ttattagcga ataaatttcc atactgagga
    78541 agggatcttg tcgtacagtt tattcatggt caattcggcg aggcggtgat ctgcggctga
    78601 gtaaaatacc gccagctcat tatctgataa ttcgtattta tttttctcga taacacgctc
    78661 cagcgtgtca attgtctggc aacgacgtaa acgcattaaa taatcggttt ttgttaaagg
    78721 tttttcggac atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat
    78781 tctctttcgt cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct
    78841 gaagatcctg catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt
    78901 cgtcgatctg ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga
    78961 aggtcgcaat atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt
    79021 taacccagcc atgattgctt tcttcaaggt ttgcaagaca gtcatgatac agggtttcac
    79081 agagaaactt aagctgcgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc
    79141 cccttcttag cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg
    79201 acttgccctc tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt
    79261 attacgaaaa attacaatta ggaaggaagc gggacaggac tcactttttc atactagcac
    79321 taacccgcag caggtaaaag cagtttgctc gaactgttca taacatttca ggattatcgc
    79381 cagatgaaat tggtaacact aataccagga ttgctctgaa tatgacgtaa taaccgagga
    79441 atgaataaag aattaccgta aataattaag aatagcctct aaatgattat ggattattaa
    79501 atctattagc gctacttaat ataaattaac taacaatcag tattttttat gaattttata
    79561 cgtgtttaat actggttttc gtatgagatc ctgagttggt ggttcaatga ctccttaatg
    79621 ttcgtcggtt atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca
    79681 atgatgatcg acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg
    79741 aaccaccaca aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc
    79801 gcccattaca ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac
    79861 cagcggcata acgccgagga taaacgccaa cgaggtcatc aggattgggc gtaaacgcat
    79921 ccgcaccgca tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt
    79981 ggcgaattcg acgataagta tcgcgttctt cgccgacaaa ccaatggttg tgagcaggcc
    80041 tacctggaag taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat
    80101 aacccccagc ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc
    80161 agccagacac aggaacacga caatcaacga aatcgcgtac agtgaaggcg cctggttgcc
    80221 ggagagacgt tcctgatagg acattcccgt ccagtcatag ccaacaccgg taggcagttt
    80281 gctcgccagt tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg
    80341 gcctaagatt tccatagatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc
    80401 ccaacgagaa gaggagaacg ccgagaacgg caccatctga ccatcagcag cacgaacata
    80461 ccagtcgccg atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt
    80521 cttcacacga ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag
    80581 agtggtgtta atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc
    80641 aatcttaaac tgcggggtat cttccagacc gtttggacgt acgctggtca acatatcagg
    80701 gtgcttcgct gcttctgcaa gcaactggtt acgcgcctga gtcagttttt cgtgaccaag
    80761 gccagcctgg tcaatcagct caaagtcaaa gccggttgcg gtacccagtt ccacgattgc
    80821 gggcaggtta aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcac gtgttgcacg
    80881 catggtaatc gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga
    80941 aacgaacgca atacctgtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa
    81001 caccgactca acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac
    81061 tttctgcgta cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc
    81121 ctggtcttca tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac
    81181 cacgatgatc agatacagca ccaggtaacg ccctgtactg cgcagaatac cgcctacgct
    81241 gtcggtgtag tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt
    81301 tttaccttcc ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg
    81361 agtcaggatc aacgccacca gtaccgacag cgccattgct gaaacaatgg taatagagaa
    81421 ctgacgatag atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga
    81481 cagtaccatc gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc
    81541 ttcttttggc ggcaaacctt cttccgccat aacacgctca acgttttcta ccacaacgat
    81601 ggcgtcatcc accaacaggc cgatggcgag caccatcccg aacattgtta gcgtgtttat
    81661 cgagaagcca aaggcggcaa ggacggcaaa ggtccccagc aataccaccg gtacggcaat
    81721 ggtcggaatc aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag
    81781 gatgatcgct tcgactagcg ttttaaccac ttcgtgaatc gagattttca cgaacggcgt
    81841 ggtgtcgtac gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag
    81901 ttcagcacgg attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc
    81961 cagaccggaa gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag
    82021 ctcaattttc gccacatcac gcagcagcac gcgggaacca tcctgattca ctttcagcag
    82081 gattttgccg aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag
    82141 ctgttggcct ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg
    82201 cgctttgatg gcggtaatga catcaaccgg cgttagctgg aatttgttca gttcattcgg
    82261 gttcatccag atacgcatcg catactgtga accgaacaac tgaacgtcac ccacgcccga
    82321 cgtacggctg atggcatctt tcatattcgc tgccacgtag tcagagatat cctcctgcgt
    82381 catggtgcca tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc
    82441 aacgctcacc ccttgctgct gtacttcttg cggcagcaac ggcatcgcca gctgcagttt
    82501 gttctgcacc tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat
    82561 ctgcacggta cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt
    82621 catattctgt tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccagg
    82681 gtaggaggcg gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag
    82741 tttgaggatc gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat
    82801 cgggcgatcg ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg
    82861 actgttcagg ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg
    82921 cttttacctg gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg
    82981 ctttcagacc ttctgtcacc agccacttat cgccgatagc ctggcttgca acgatcggac
    83041 gggtttccac tttgtcatcc gcgccaacca ccagtacggt ggcatcgcca cgcggcgtac
    83101 gggttacgcc ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg
    83161 cccgcacgaa catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg
    83221 tgatagaccc ggtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg
    83281 ggaacttaat gccgtcactg gtgatcagcg acactttggc tttgccgttt tcttgtttca
    83341 acgtgccatt cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat
    83401 caacgtagat cggatcaagt tgctgcacgg ttgccagcgt agtcgcctga ccgttctgta
    83461 ccaatgcgcc ttccgtcacg ttcgacttac caatgcgacc gctaatcggc gaggtgactt
    83521 tggtgtaagc cagattgatc cgcgcagttt caacggcagc tttcgccgca gtgaccgcag
    83581 cattcgcctg ttgcgcatca gccagagcct gatcgtactc ttgtttactg atgtactgag
    83641 taccgagcag tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg
    83701 ctttcgccaa atcacctttc gcactgtcgt atgccgcctg ataggtcgca ggatcaatct
    83761 gatagagaga gacacctgct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa
    83821 tcccgctaac ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct
    83881 cggttgtgat ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct
    83941 ggccaccttg ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca
    84001 tcagaacgac cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt
    84061 ccgatttcaa attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc
    84121 gtcgtgctat ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga
    84181 acatatggca cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt
    84241 ggctctacgt cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa
    84301 agcagctggc gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt
    84361 cagtgagatc tgggaactgt cagaatccaa tattggtgaa ctcgagcttg agtatcaggc
    84421 aaaattccct ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc
    84481 cacggtgaca gaagaacggc gtcgattatt gatggagatt atattccaca aatgcgaatt
    84541 tgtcggagaa atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga
    84601 ccgtatagaa caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat
    84661 gacgcgtcgg gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct
    84721 ctttgccccg caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact
    84781 ggagatgtat ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccttgaat
    84841 ctgactccag gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg
    84901 atattcttgc ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca
    84961 tgactatgtt ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt
    85021 ttgtttttgt cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc
    85081 tgccgacaaa agcggacctg caggcacaac ttgactcact gaataaacaa aaagatcttt
    85141 ctgctcagga caaactggtg cagcaggatc tgacagatac attagctacc ctcgataaaa
    85201 tcgatcgcgt aaaagaagag acagttcagc tacggcaaaa ggttgctgaa gcgccggaaa
    85261 aaatgcgcca ggcgaccgcg gcgttaactg cacttagcga tgtcgataat gacgaagaaa
    85321 ctcgcaaaat tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc
    85381 tggacgattt gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt
    85441 tacagacgca gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac
    85501 aaattcgcag tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga
    85561 aagtgttaat gcaggctcag caggctttgc tgaatgccga gattgaccag cagcgtaaaa
    85621 gcctggaagg gaacacagtc ttacaggata ccttgcaaaa gcaacgggat tacgtgacgg
    85681 cgaacagcgc ccgtctggag caccagttgc agctgttgca agaagcagta aacagcaagc
    85741 gcttgacttt aaccgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta
    85801 ttcaggctaa tccgctggtg aagcaggaac tggaaattaa ccagcagtta agtcagcgtc
    85861 tgattaccgc gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact
    85921 ggctggagcg ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg
    85981 gcagcctgct gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg
    86041 aactggaaaa catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc
    86101 agcagcgtga tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca
    86161 ccaacgaagt caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg
    86221 aattgctgga tcagcttaac aaacagttgg gtaaccagct gatgatggcc attaacctgc
    86281 aaatcaacca gcagcagtta atgagtgtgt cgaaaaacct gaaatccatc ctgactcagc
    86341 aaatcttttg ggtgaatagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc
    86401 aaagcctgag agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc
    86461 ctgccgtttt tatcgctttc ctcgctggtt tgccgctgct gttgattgcc gggctgatcc
    86521 actggcgtct gggctggctg aaagcgtatc aacaaaaact ggcttccgct gtgggttccc
    86581 tgcgtaacga cagccagctc aacacaccaa aagcgatcct tatcgacctg atccgtgcgc
    86641 tgccggtgtg cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca
    86701 tcagcgaact gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc
    86761 tgtgctggaa agtgttggag aaaaacggcg ttgccgtgcg tcactttggt atgccggaac
    86821 agcagaccag ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc
    86881 atttctggtc tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc
    86941 aggcgatgat tttcttcaac ttgctgctga ttgccttcct ggtgtggccg atgtgccgcg
    87001 aaagctggcg tgataaagag tcgcacacca tgcgactggt caccattacc gtactgtcga
    87061 ttatcccgat tgcgttgatg gtgctgaccg ccacgggtta tttctacacc acgctgcgcc
    87121 tctccgggcg ttggattgaa accgtttatt tggtgatcat ctggaacctg ctgtaccaga
    87181 cggtattgcg cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc
    87241 gtcggcagaa tctggtgaaa gagggcgcag aaggtgctga accgccggaa gaacccacca
    87301 ttgcactgga gcaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc
    87361 tgttcggtgt catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctgg
    87421 acagcatcac gctctggcat tacaacggca ctgaagctgg tgctgcggtg gtgaaaaacg
    87481 tcaccatggg cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc
    87541 gcaacctgcc tggtttgctg gaagtgttgg tgctctcgcg cctgaacatg cgccagggcg
    87601 cgtcgtatgc cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg
    87661 tgttcggatc gctgggcgtc tcgtgggata aactccagtg gctggcggca gcattatccg
    87721 taggtcttgg ttttggttta caagagatct tcggtaactt cgtttccggt ttgatcattc
    87781 tgttcgaacg tccggtgcgc ataggcgaca cggtaaccat tggtagcttc tcggggacgg
    87841 taagtaagat ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca
    87901 tcccgaacaa agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacacgacta
    87961 cgcgtctggt gatccgtctc ggcgtagctt atggctccga tctggaaaaa gtgcgtaaag
    88021 tgttgctgaa ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct
    88081 tctttacggc atttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac
    88141 tgcgtgaccg tagccgtact gtcgatgagc tgaaccgtac tatcgatcag ttgtgccgtg
    88201 aaaacgacat caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg
    88261 atgaggtgac ggaagtcaaa cgcgactaca aaggcgacga tccgacgcca gcggtagggt
    88321 aataaccaaa gggggcgaca tttagttgcc ccgagattac taacaaagtg cgcgttgttc
    88381 atgccggatg cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatatatt
    88441 gcaggagcgg tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa
    88501 tcttagggga catttagcgg ccccatttat ttctcacttt tccgcctcat catcgcgcgt
    88561 taatttcttt tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg
    88621 gatctgattt tcttccagta gcacaatcaa atcgacggcc agtttgacat cgtcaggggc
    88681 attttccagt gacatactct ctccattgct agcgggttaa acgcgctaac ctgttttcga
    88741 ttttttccag cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat
    88801 gcagcgtctg ctgttccacg agatcggtcg ctcgtgccag acgggctcta cgttcggcaa
    88861 ccatctcacg tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg
    88921 caattttcgg tggtgcgcta tcccactcgc gtagcgacca ggctgtggct tcacgcgcta
    88981 tagcctccag ttgcgccgcc agatgttctg ccagccaggc gacttgcggc agctgttgct
    89041 gctcaaccgc atgacgaagc gcagccagat tatcgcccgc ctcatcgaga caagcctgca
    89101 gtgttgtcgc acgagtctga aaaagatgcc tgtcaaaacg ggcgcttagc gtggcaaatt
    89161 gcgctaccgg ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca
    89221 gcagggcggt tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagca
    89281 actctgtaag ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct
    89341 ggcggtagtg ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat
    89401 cctgctggcg gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta
    89461 ccgctcatgg tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg
    89521 cgtcaaaccg cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt
    89581 ccagatgcca tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttttatat
    89641 gtggcgaatt ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt
    89701 tgcaattatt gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca
    89761 aaggttaaac aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca
    89821 agcaggcata cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc
    89881 aaaagcattc aggactaccc aaaacccggc attcttttcc gtgatgtcac cagcttactg
    89941 gaagacccga aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg
    90001 ggcattacca aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct
    90061 ctgggtctgg gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc
    90121 atcagtgaaa cttacgacct ggaatacggc accgatcagc tggaaatcca cgttgatgcc
    90181 atcaaaccgg gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc
    90241 gaagcgaccg ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt
    90301 atcaacctgt tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac
    90361 agccttgtcc cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga
    90421 cagcacaaga tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt
    90481 cgtgaatcca ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa
    90541 aatggcgccc acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg
    90601 cgaacggctt gtcgttaggg cgtattcatc atgcttatct tttttccggt acccgtggcg
    90661 tcggaaaaac ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta
    90721 ccgcgacgcc gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg
    90781 atctgattga aatcgacgcc gcctcgcgca ctaaagtcga agatacccgc gacctgctgg
    90841 ataacgttca gtacgctccg gcgcgtggtc gtttcaaagt ttatctcatc gacgaagtgc
    90901 atatgctgtc gcgtcacagc tttaacgcac tgttaaaaac ccttgaagag ccgccagagc
    90961 acgttaagtt tttgctggcg acgaccgatc cgcagaaatt gccggtgacg attttgtcac
    91021 gctgtctgca atttcatctc aaggcgctgg atgtcgagca aattcgccat cagcttgagc
    91081 acatcctcaa cgaagaacat atcgctcacg agccgcgggc gctgcaattg ttggcacgcg
    91141 ccgctgaagg cagcctgcga gatgctttaa gtctgaccga ccaggcgatt gccagcggtg
    91201 acggccaggt ttcaacccag gcggtcagtg cgatgctggg tacgcttgac gacgatcagg
    91261 cgttatcgct ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctaatta
    91321 atgaagctgc tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggtctgt
    91381 tgcatcgtat tgcgatggta caactttcgt ctgctgctct tggcaacgac atggccgcca
    91441 ttgagctgcg gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact
    91501 atcagacgct gttgattggt cgcaaagaat taccgtatgc gccagaccgc cgtatgggtg
    91561 ttgagatgac gctgctgcgc gcgctggcgt tccatccgcg tatgcctctg cctgagccag
    91621 aagtgccacg ccagtccttt gcgcccgttg cgccaacggc agtaatgacg ccaacacagg
    91681 tgccgccgca accgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    91741 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    91801 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    91861 gactggcttc ggtcaccgat cgcgttcagg cgcgcccggt gccatcggcg ctggaaaaag
    91921 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccagtgatg cagcaaaaag
    91981 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    92041 tggcggcgaa gctggcggca gaagccattg agcgcgaccc gtgggcggct caggtgagtc
    92101 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
    92161 acaactcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggcgcac
    92221 agcaaaaact ggctgaagcg ttgagcacgt taaaaggttc aacggttgaa ctgactatcg
    92281 ttgaagatga taatcccgcg gtgcgtacgc cgctggaatg gcgtcaggcg atatacgaag
    92341 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    92401 gattcttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    92461 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    92521 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    92581 atcgcgcagc tggaagtcac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    92641 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    92701 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    92761 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    92821 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    92881 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    92941 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    93001 ccgattgccg caccttcacc gaacaggaag tctgtaacat ctgttcgaat ccacgtcgtc
    93061 aggaaaacgg tcaaatctgc gtggtggaga gtccggcgga catttacgcc attgagcaga
    93121 cggggcagtt ttcaggtcgt tattttgtgt tgatgggaca cctgtcaccg ctggacggca
    93181 tcggtcctga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    93241 ctgaagtgat cctcgccacc aaccccacgg ttgaaggtga agctaccgct aactacattg
    93301 ccgagctttg tgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    93361 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ttcccttgcc gggcgtcata
    93421 agattcgttt ttaagcaaac gagagcagga tcacctgctc tcgcttgaaa ttattctccc
    93481 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    93541 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    93601 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    93661 tccgatgcgg cggacaagct gcgtttccgc gcgctttcta acccggacct gtacgaaggt
    93721 gatggtgaac tacgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    93781 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    93841 tccggcacca aatcattcct cgaatccctg ggttctgacc aggcgaaaga cagccagctg
    93901 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggctgacaa agtgaccgtg
    93961 cgtactcgcg cagcaggcga aaaaccagaa aatggcgtct tctgggaatc ggctggtgaa
    94021 ggtgaataca ctgttgccga catcaccaaa gaagatcgtg gtactgaaat caccctgcat
    94081 ctgcgtgaag gcgaagacga gttcctcgat gactggcgtg tgcgttccat catcagcaaa
    94141 tattccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agacggcgaa
    94201 accgttatct cctgggagaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    94261 gaaatcaccg atgaagagta caaagagttc tacaaacaca tcgcccacga ctttaacgat
    94321 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    94381 attccgtctc aggcaccgtg ggatatgtgg aaccgcgatc ataaacacgg tctgaaactg
    94441 tatgttcagc gtgtgttcat catggacgac gcagaacagt tcatgccgaa ctatctgcgc
    94501 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    94561 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    94621 ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttttggca acagtttggc
    94681 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    94741 ctgcggtttg cgtctaccca taccgattct tctgcgcaga ccgtgtctct ggaagactac
    94801 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga cagctacgcg
    94861 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    94921 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    94981 ttccagtctg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    95041 agcgcgaaag aagcggagaa agcactgact ccgtttatcg accgtgtgaa agccctgctc
    95101 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    95161 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    95221 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    95281 gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct gctggatcag
    95341 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaac
    95401 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    95461 tcatcatctg cactttccgc aaattatctc gccattaacc gtttcagccc caggtgcctt
    95521 tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa ttcgacacat
    95581 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    95641 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    95701 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    95761 gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    95821 gaagactgcc gcaacggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    95881 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    95941 ctgattgttg accgtatcgt aggccgccgc gttcacgcgc cgtctggtcg tgtttatcac
    96001 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agagctgact
    96061 acccgtaaag atgatcagga agagaccgta cgtaaacgtc tggttgaata ccatcagatg
    96121 actgcaccgc tgatcggcta ctactctaaa gaagcggaag cgggtaacac caaatacgcg
    96181 aaagttgacg gcaccaagcc ggtggctgaa gttcgcgcag ctctggaaaa aattctcggc
    96241 taatcaagcc gcagcagatc tgctttcggg caggtctgct catcctcctc aggcaatacc
    96301 tctcacagca attagttctt cttcttcact tttccgctac aattatcaac aagttgaatc
    96361 gataagaggc ggtaatgcgt cagacaaaaa ccggtatcct gctggcaaac ctgggtacgc
    96421 ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac
    96481 gtgtggttga tacctcacgg ttgttatggt ggccattgct gcgcggcgtg attttgccac
    96541 tgcgctcgcc gcgtgtggcg aagctgtatg cctctgtctg gatggaaggt ggctcgccgc
    96601 tgatggttta cagccgccag caacagcagg cgctggcaca acgtttaccg gagacgcccg
    96661 tagcgctggg aatgagctac ggctcgccat cactggaaag cgccgtagat gaactactgg
    96721 cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttcaacgg
    96781 tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga
    96841 tatcgtttat tcgtgattac gccgataacc acgattatat taatgcactg gcgaacagcg
    96901 tacgtgcttc ttttgccaaa catggcgaac cggatctgct gttgctctct tatcatggca
    96961 ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgtcgc acaacgactc
    97021 gcgaactggc ttccgcactg gggatggcac cggaaaaagt gatgatgacc tttcagtcgc
    97081 gctttgggcg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag
    97141 aaaaaggtgt aggtcatatt caggtgatgt gcccgggctt tgctgcggat tgtctggaga
    97201 cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc gggaaaaaat
    97261 acgaatatat tccggcactt aatgccacgc cggaacatat tgaaatgatg gctaatcttg
    97321 ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt
    97381 cggcggtttt catcatccgt gaataatgca aaaaggcgtg cagcgtgcct gggtagagtt
    97441 tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg ctgtcatcca
    97501 gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat
    97561 tattaaacag gcagtaatac ggcgactcgc ggtccgcgtc gttgcttaaa tacgcctctt
    97621 catacatctg taaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag
    97681 tcacggaatc ccgtaatccg taaagcccat accacagcaa aacgcccgca actttaccgc
    97741 aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccaacatg gcacctgcgg
    97801 aatcaccggt aaagccaatg cgggacatat tgatttgata atcttccgcc tgctggtgga
    97861 aataacaaca ggcagccaca atttcctcta ttgcttgcgg aaaacgcgct tcaggtgaaa
    97921 gggtgtaatc aatgccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc
    97981 gatcgtgggt atcaagattg ccgagaataa aaccgccgcc atgcaaataa aatagcgtcg
    98041 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    98101 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    98161 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccagggcggt aaatccggct
    98221 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaacg ggtaatttgt
    98281 tttccggctt catgcaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    98341 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    98401 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggca
    98461 aacgtaaatc caaacattat tttcccgtaa acgcacgcga tccgctgctt cagcaattcc
    98521 agccagaaaa cgaaaccagc gctgcctggg tagtgggtat cgatcaaacg ctggtcgata
    98581 ttgaagcgaa agtggatgat gaatttattg agcgttatgg attaagcgcc gggcattcac
    98641 tggtgattga ggatgacgta gccgaagcgc tttatcagga actaaaacag aaaaacctga
    98701 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    98761 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagctatg
    98821 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    98881 atggcccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    98941 tcagccccgg ccacatgaac cagctgcggg ctgaaagtat tccggaagat gtgattgccg
    99001 gagcctcggc tctggttctc acctcttatc tggtgcgttg caagccgggt gaacccatgc
    99061 cggaagcaac catgaaagct attgagtacg cgaagaaata taacgtaccg gtggtgctga
    99121 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    99181 accacgtctc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    99241 atccgttgtt ggcatctgac aaggcgctgg actgggttga tctggtgctg tgtaccgcag
    99301 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    99361 atccgctgct gccgggtgct atagcggaat ttaaccagta tgagtttagc cgcgccatgc
    99421 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    99481 ggccggaaaa aatcatgaac actaatggag caggggatgg cgcactggca gcgttgctgc
    99541 atgacattac cgccaacagc taccaccgta gcaacgtacc aaactccagc aaacataaat
    99601 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    99661 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    99721 gcctggaaga gtcttactgg gatcgttaag ttatcgacca gaccggacga cgatggcatc
    99781 cgtaggccgg ataagatgcg tcagcatcgc atccggcaat ggaatcaatg ttttaccccg
    99841 tcaccacctc acccgccggt ggcgtttcca gcagttccag catagtacga gcaatttcac
    99901 gctcacccat cactacctga ttcgcaccac gttcggtgat atacgccact tcatcgtcat
    99961 aatgggcgcg ggcaataatc tcaatatccg gatttttcgc gcgggcagat gccacaatct
   100021 cgcccgcttc ataaccgttg ggaatcgtca ggatcagcca ttttgcacat tccagatgcg
   100081 ccagttgcat aatttcttcg ttcgccgcat tgcccaatac tgcgcggacc ccgcgctctc
   100141 gcagttcatc aacacgggtt cgtgacgtct caatcaccac cagcggaata tcagaggcga
   100201 gcaatttctc ccccagcagg ctgcctacac gaccgtaacc taccagtagc gcatggttgc
   100261 aaatatccac tgggatctgc ttctcttctt cgatagcctc ttccagcgtc tgctcttcca
   100321 gcgtttcggt cttcgccaga tatttctcca gcagcgcaaa caggaccggg ttgagcataa
   100381 tcgacaaaat cgcccctgcc agtaccaggt tctgcccggc ttgcggcaat agatccagcg
   100441 ccatgcccag tcccgccagg ataaacgcga actcaccaat ttgcgccagg ctggcggcga
   100501 tagttaatgc cgtacgttgg gagtgaccaa acagtcgcac caggaaaaac gcggctaacg
   100561 acttaccaaa cagaataatc gccagcgtcg ccagcactgc cagcggttgc tgaatcagaa
   100621 ttaacggatc aaacaacatc ccgacggaga caaaaaacag caccgcaaac gcgtcgcgca
   100681 atggcagcgt atcgtgggcg gcacggtgac tcagttcaga ctcgttcagt accatcccgg
   100741 caaagaacgc accgagtgca aaggagacat caaacagctc caccgcacca aaggcaatcc
   100801 ctaacgccag cgccagcacc gacagggtaa acagctcacg ggaaccggtt gctgcgctac
   100861 gtgccataat ccacggcacc agacggcgac ctaccagcat cataatggcg ataaatgcga
   100921 tcaccttgcc gatggtgatc cccatatcga cagcaagagt ggcaaagccc acatcgcctt
   100981 gttccatcat tcctgccact gcgggcagca acaccagcgt cagaaccatg accaggtctt
   101041 ccacaatcaa ccaaccgatg gcgatttgcc cacgctgact gtcaattaat tgccgttctt
   101101 caagtgcgcg cagtaacacc acggtactgg cggtggaaag gcataagccg aacacgatac
   101161 cggtcattaa cgaccagccc agcacggcgg agagcgccat acccagcagc gtcgccacgg
   101221 ctatctgggc gatcgcaccg ggaatggcga tggcctttac cgccatcaaa tccttcagcg
   101281 aaaagtgcag acccacgcca aacatcaaca gaatgacgcc cagttcagcc agttccggcg
   101341 caagcttggt atcggcaaca aagcccggag tgaatggtcc tgccagcaca cccgctaaca
   101401 gatatcccac cagaggagaa atacgtagtt tattggccag catgccgagg ataaaggcga
   101461 gcacaaggcc gccaacaatg gtggtgataa gcggggtggc gtgatgcatt ccgtctcctt
   101521 ttcctggtgg ttattgtcca tttttggccg ggaaaaccaa aattacaggt aatagtttat
   101581 gacaatttca ttgatgatgt ttatgaataa ttgttgaatt ttgcagaaaa atggaattag
   101641 ctgcaaaaaa agcacggatc ggaaaacaga aggcggttta acagaggaaa ggcttatggc
   101701 gactgtaaaa cgatgcagcc aaagtttgcc tttggctgca atgaaatcag tctttatgcc
   101761 ggttatcagg caggaatatg gtcaacatcc ccaatagtgg caggaaagca cagattttat
   101821 agactaactc gatgctggtg tgatcggcga taagccccag aactgccgct cccagacctc
   101881 ccatgccaaa agcaaaaccg aaaaagagtc cagaaaccat accgatacgt cccggaagta
   101941 gctcctgagc gtagaccaga atggcagaga atgccgaagc gaggataaat ccaataatca
   102001 ccgttaaaac ccccgtccag tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa
   102061 cgccgaggat agagccccaa atcacatatt tccgtccaat tttatcccct acaggcccgc
   102121 caatcaccgt acctgccgca acggcaaaca ggaaggcaaa caaatgaagc tgagcattct
   102181 ggatagataa tccgaatttt tgcatcagat aaaaggtgta atagctgctg atgctcgcca
   102241 tatagaaata tttcgagaaa atgaggatta acagaatgct gaccgccaga acaactttat
   102301 tgcgcggcag tggattgata atcgtcgctt tgggttttcc tttattcatt cggtgctgtg
   102361 ccgagtacca acggctgatt tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa
   102421 accaggcaac gttgcctttg ccataaggcg cgataatcac cgccgccagc aaggggccca
   102481 gggaactgcc aaagttgccg ccgacctgaa agatagattg cgccaggcca tgacgcccgc
   102541 cggaagccat acgggccacg cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga
   102601 ccagcgccgc tgccagcaga actgcgccga aactgcccgc cagcgccagc agcaccagac
   102661 cacttaaggt aaagcacatg ccaattggca acgaccacgg catcggatat ttatcggtcc
   102721 agtagccgac aactggttgc agtagcgaag aggcgagctg gaaggtgagg gttatcatgc
   102781 caatctgcat aaatgtcaga gaaaactctg actgaagcag cggataaatc gccagaatca
   102841 gcgattggat catgtcgttc agcagatgtg agaggctgat agcacctaaa ataccaaacg
   102901 atgttcgggc cttggtcgtt gacgcagccg cgcccgccac aggctgggtt tgttcactca
   102961 ttgccatagg aaagtcactt tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt
   103021 attacccgac taacatacct gtatgcgccg cctgaaggaa gtctcaacgc tgaatacaga
   103081 atttctaatc tggatacaga tttatcttca ccggacgcag acttgtctat gatgtcgcgt
   103141 catactattt ttcaacacgt tgaaatcagg tcagggagag aagtatgaaa ttattgcagc
   103201 ggggcgtggc gttagcactg ttaaccacat ttacactggc gagtgaagct gctctggcgt
   103261 atgagcagga taaaacctac aaaattacag ttctgcatac caatgatcat catgggcatt
   103321 tttggcgcaa tgaatatggc gaatatggtc tggcggcgca aaaaacgctg gtggatggta
   103381 tccgcaaaga ggttgcggct gaaggcggta gcgtgctgct actttccggt ggcgacatta
   103441 acactggcgt gcccgagtcc gacttacagg atgccgaacc tgattttcgc ggtatgaatc
   103501 tggtgggcta tgacgcgatg gcgatcggta atcatgaatt tgataacccg ctcaccgtat
   103561 tacgccagca ggaaaagtgg gctaagttcc cgttgctttc cgccaatatc taccagaaaa
   103621 gtaccggcga gcgcctgttt aaaccatggg cgctgtttaa gcgtcaggat ctgaaaattg
   103681 ccgttattgg cctgacgacg gatgacacag ccaaaattgg taatccggaa tatttcactg
   103741 atatcgaatt tcgtaagccc gctgatgaag cgaagctggt gattcaggag ctgcaacaga
   103801 cagaaaagcc agacattatt atcgcggcga cccatatggg gcattacgac aatggtgagc
   103861 acggctctaa cgcaccgggc gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg
   103921 cgatgatcgt cggtggccac tcgcaagctc cggtctgcat ggcggcagaa aacaaaaaac
   103981 aggtcgatta cgtgccgggt acgccatgca aaccggatca acaaaacggc atctggattg
   104041 tgcaggcgca tgagtggggc aaatacgtgg gacgggctga ttttgagttt cgtaatggcg
   104101 aaatgaaaat ggttaactac cagctgattc cggtgaacct gaagaagaaa gtgacctggg
   104161 aagacgggaa aagcgagcgc gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga
   104221 tctcgctgtt atcaccgttc cagaacaaag gcaaagcgca gctggaagtg aaaataggcg
   104281 aaaccaatgg tcgtctggaa ggcgatcgtg acaaagtacg ttttgtacag accaatatgg
   104341 ggcggttgat tatggcagca caaatggatc gcactggtgc cgactttgcg gtgatgagcg
   104401 gaggcggaat tcgtgattct atcgaagcag gtgatatcag ctataaaaac gtactgaaag
   104461 tgcagccatt cggcaatgtg gtggtgtatg ccgacatgac cggtaaagag gtgatcgatt
   104521 atttgaccgc cgtcgcgcag atgaagccag attcaggtgc ctacccgcaa tttgccaacg
   104581 ttagctttgt ggcgaaagac ggtaaactga acaacctgaa aatcaaaggc gaaccggtcg
   104641 atccggcgaa aacttaccgc atggcgacat taaacttcaa tgccaccggc ggtgatggct
   104701 atccgcgcct tgataacaaa gcgggctatg tgaataccgg ctttattgat gccgaagtgc
   104761 tgaaagcgta tatccagaaa agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg
   104821 aggtgagctg gcagtaatcc gaaagtgccg gatgttcgca tccggcacaa tgcttaatcg
   104881 cgacgggcga tatcagcaaa tttggcatcg aggatctttg ccagatcgcc tgccgccagt
   104941 tcgatatcca gcccgcgctt gccgccggaa acataaatag tggcaaattc ttgtgcgggg
   105001 gcgtcgataa tcgttggcag acgttttttc tgccccagtg ggctaatccc ccctaccaga
   105061 taccccgtcg aacgctgcgc gaccatcgga tcggccatct caactttctt ggcacccagc
   105121 gcttttgcta cttttttaag atccagttga cctgcgaccg gcgtaacggc cacggcaagg
   105181 tgtttcatat caccgttcac tgcgaccagc agcgttttgt agacctgatc cggattcaaa
   105241 cctaattttt tgacgacttc atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggtg
   105301 tggatttgaa acgaaatctt gtttttttcg agtaatttaa ctgcgggtgt catggtaatt
   105361 ttcctgcctt aaacaaaaaa acgaagcaag catacgcctg atggcggtcc aaaaaaaaga
   105421 gtcatcttgc ctaagagtat tggcaggatg gtgagattga gcgacaatcg agttacaccg
   105481 tcgcttaaag tgacggcata ataataaaaa aatgaaattc ctctttgacg ggccaatagc
   105541 gatattggcc atttttttag cgcaacatct gcggcaaatt cccttctcca tacaggtgta
   105601 gtgcaccaac cgcgaccaca tagcgccccg gcggcatggc gcataatttg tcccgccagg
   105661 cgagatttcg ctgatgcatc agcacatcgt acagcgactg actgaacgta ttaggcagcg
   105721 ttatttcatt attttgcggc ggcgcattca gccaccagct catcatttgt tgcagcaacc
   105781 gtgcgttggt gtgccagtgg gtcagcgtat cgtccagcag cgccagtccg ttgtcaggga
   105841 gctggagcaa catggcaatc tggttttcag ccccttccag ttcaatcacg ggtttatgtt
   105901 gttgcttagc cgcctgcaat agctggtaat cgataccgta ttctgcccgc agccccagtt
   105961 tttgcgcctg cgtcgcctga agaaccatcg cgatttgcca cagcggttga gtagaaaaga
   106021 gcgacggaga aatgcccatc tcctggctaa tatgctgcag gttttggagt tgctcctcgc
   106081 taatgcgctt ttccagggcc tcgcaggcag gcaaattggc aaaaggcgta tcgctggtgg
   106141 aaacatccgc ctcgacgatc agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg
   106201 gcaggggagc catatcgtgg ctacccatat gaatactgcc gatcagatga aaatggcgat
   106261 tgccgggaag agagatatcg atagcaggcc aggtgtaatg attaccgcgc agcgcggccc
   106321 aaagcgtttt tacccggtac aacagatcca tacgacctcc ctttgtgaaa tatcatgcta
   106381 gcgcgcgggg agggatggcg caaatggagt tgattgttgc acttgcgcac agtgcttgtg
   106441 tgcattacaa tgattaaata atggacaagg agatgagaga ggaatggctc aaaagaaaaa
   106501 tataaggagt tttcgtgatg cctggttggc tgattttttt gtccattcaa cgccacatcg
   106561 aaagatccct gctgaaatcc ataccacgtt atcacgaaag ttggacatca ttaatgctgc
   106621 cacttctcat cgggatttac gctcccctcc ggggaaccga tacgaagagt tatccgggaa
   106681 attacaggaa tactcctcca ttagagtgaa taaacagtac cgactgattt ttaaatgggt
   106741 taatggcaag gccgaagaat tgtttttaga cccacacaac tactaaaaca tagctgtcgt
   106801 attgacgaga aaggtaaaac tatgaaacag gcaacaagaa aaccgacgac acccggagat
   106861 attcttctct atgaatatct ggaaccgctc gatttgaaaa tcaatgagtt agcagagttg
   106921 ctgcatgttc atcgtaatag cgtcagtgca ctgatcaata acaatcgtaa actcaccact
   106981 gagatggcat tccgtctggc gaaagttttt gataccacag tcgatttttg gctaaacctc
   107041 caggcggcag ttgatctttg ggaagttgaa aacaacatgc gtacccagga agaattggga
   107101 cggattgaaa cagtggctga atatttggca cgccgtgaag agcgtgcaaa aaaggtcgcg
   107161 tagaataatt tccctataac ggatgcgcca ccctaaagca gtgcatccgc aatgatgtac
   107221 ttattccttc ggtttaaacc gcagcaaccg gttggcgtta ctcactacgg taatcgacga
   107281 gagcgccatt gccgctccgg cgaccaccgg gttaagcagc gtaccggtga acggccacaa
   107341 aataccggcg gcgaccggaa taccaatgct gttgtagata aacgcaccga gcaggttctg
   107401 cttcatattg cgcagcgttg cgcgggaaat cgcgagcgca tccgcaacac ccatcaggct
   107461 atggcgcatc agggtaatcg ccgcggtttc aatggcaaca tcactgccgc cacccattgc
   107521 aatgccgaca tccgcctgag ccagcgctgg cgcgtcgtta atgccatcgc ccaccattgc
   107581 cacctgacgt ccttcacttt gcagacgttt gatcgcttcg gctttaccgt ccggcagcac
   107641 cccggctatc acctcatcaa tccctgcttc tttggcgatc gcattggcgg tggttgggtt
   107701 atccccggtt agcatcacca gacgatatcc cgctttatgc aggcgttgca gcgccgccac
   107761 gctatcacta cgcaatggat cgcgtacagc cagcagggct accgctttcc cgtcaaccgc
   107821 cagcagcaca ggcgttgccc cttgcgatgc ctgagctgaa atatctgctt caatcgcttt
   107881 agtatcaact tgctggtcat ttaacagcgc ctgattgccc agcaataacg catgaccttc
   107941 agcttcaccg ctcacgccca gcccgcgcaa tgtgcggaaa ccgttgacct gcggcagctg
   108001 catatcgctt gctttatcga ggatcgcccg tgccagcgga tggctggaac cttgttccag
   108061 tgccgccgcc agacgcagcg cctgcgcttc gtcaaaatca gcaaatgttt ttactgcgac
   108121 aacctgcggc ttcccttcgg tcagcgtccc ggttttatcg aacaccacgg tgtcgagcgt
   108181 actggcgcgt tgcagcgcgt cggcgtcccg caccagcacg ccaaactcag ccgcccgccc
   108241 gacgcccgaa ataatcgaca tcggcgtcgc cagtcccagc gcacacggac aggcgataat
   108301 cagcaccgtg gtggcaatca ccagggtata aacaatttgc ggtgccgggc caaagaaata
   108361 ccagattgcc gcactgacca gcgcaatgac taccaccacc ggcacaaaca cagcggagat
   108421 tttatccgcc agttgaccga tttctggctt gctgctctgg gcctgtcgca ccatgcgaat
   108481 gatccgtgac agcgtagtat ggctgccgac cgcactggcg cgaaacagca cactgccgtc
   108541 ctgtaccact gtcccggcat ggacgctatc gccttcgcct ttttgctgcg ggattggttc
   108601 gcccgtcagc atcgcttcat ccagccacgc ttcgccctgg gtaatttcac catctaccgg
   108661 cacccgatca cccgtcgtca ggcgcagcaa catacctggc tgaacatctg ccagcggcac
   108721 gtttttttca ccttcgttgg tgaccaggcg cgccgtcggt ggggttaaat cgagtaattt
   108781 ctccagcgcc ttagaagaac gctgacgtgc gcgcgcttcc agcatatgac cgagattaat
   108841 cagaccgata atcatcgcac tggcttcata gtaaagatgt cgcgcttcca tcgggaacca
   108901 ctgcggccac aggttgacgc tcattgaata gagccacgcc acgcccgtac ccagcgccac
   108961 cagcgtatcc atcgtcgctg caccgttcag caggcttttc catgcgctgc ggtaaaaatg
   109021 gccgccggcg aaaaccatta ccgccagggt tatcaggccg ataaccaacc acaggctgcg
   109081 gttgtcggcg gtgaccatca tgttatcgcc gatcatcccc cagaccatca ccgggatacc
   109141 caccgccagt gcgacaatcg cctgccagcg gaagcgcttc atcgtagcga cggcggtttc
   109201 ttgctggcgc tcgcggcgtt tagcgtcatc ttcaatcgct tccgcgccgt agcccgcttt
   109261 ttccaccgcc tgcactaaat cttgtgggga ggcactgccc atcaccagcg cagtacgctc
   109321 cgccaggttt acccgtgcct gagtgacgcc cggtacgctt tgcaacgcat tttgtacgcg
   109381 ggtgacacag ctggcgcagc tcatgccgct cagcagcaac tgctggctgt catcgtcatc
   109441 ggcggtcgct gccggaagcg cctcagaaac cgctgtcagt gcttccgacg ggattgatga
   109501 ctccgccagc ggtttagcct ttgggtggct tacagatgcg tcataacccg cttgtttgat
   109561 ggtttcgatc agctgttctg cactggcagt cccggtaacg tgcgcttcag tgacagacac
   109621 atccgcctgc tcaacatccg ggcgctgttc gagactttct ttcacgcgtt taacgcagtg
   109681 accgcaggac aggccgtcca gggtcaggtc gatagtttgt gacataaaac actcctttta
   109741 agacagtttt gactggctgt gataaaggtt aaaccttcca gcaaggggaa ggtcaagaaa
   109801 ttaataaacc aggcgggtaa aagtacgtaa agattaaaaa atcggctcga tttgcatcag
   109861 gattagacat ttatctcttt gttttcctgt agttaagttg cgggtgctaa gttaaatcag
   109921 gatgcctgaa aattggcacc ggggtgagga attacctccc gcatctataa aaaggagtta
   109981 acaaaagatg ttagatgcaa acaaattaca gcaggcagtg gatcaggctt acacccaatt
   110041 tcactcactt aacggcggac aaaatgccga ctacattccc tttctggcga atgtacctgg
   110101 tcaactggca gcagtggcta ttgtgacctg cgatggcaac atctatcgcg cgggtgatag
   110161 tgattaccgc tttgcgctgg aatccatctc gaaagtctgt acgttagccc ttgcgttaga
   110221 agatgtcggc ccgcaggcgg tacaggacaa aattggcgct gacccgaccg gattgccctt
   110281 taactcagtt atcgccttag agttgcatgg cggcaaaccg ctgtcgccac tggtaaatgc
   110341 tggcgctatt gccaccacca gcctgattaa cgctgaaaat actgaacaac gctggcagcg
   110401 aattttacat atccaacaac aactggctgg tgagcaggta gcgctctctg acgaagtcaa
   110461 ccagtcggaa caaacaacca acttccataa ccgggccatt gcctggctgc tgtactccgc
   110521 cggatatctc tattgtgatg caatggaagc ctgtgacgtg tacacccgtc agtgctccac
   110581 gctcatcaat actgttgaac tggcaacgct tggcgcgacg ctggcggcgg gtggtgtgaa
   110641 tccgctgacg cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc tggctgaaat
   110701 gatgatggaa gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg gtttaccggg
   110761 caaaagcggt gtaggtggcg gtattctggc ggtcgtccct ggcgtgatgg gaattgccgc
   110821 gttctcacca ccgctggacg aagaaggcaa cagtgttcgc ggtcaaaaaa tggtggcatc
   110881 ggtcgctaag caactcggct ataacgtgtt taagggctga tcatgatgaa cacggaaggt
   110941 aataacgata acaaacccct cggtctatgg aacgtcgttt ccatc
//
LOCUS       DAZGHF010000002       104927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGHF010000002 DAZGHF010000000
VERSION     DAZGHF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 104927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.002"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(114..490)
                     /gene="rnpB"
                     /locus_tag="ACHK3A_000107"
     ncRNA           complement(114..490)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHK3A_000107"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(523..1668)
                     /gene="garK"
                     /locus_tag="ACHK3A_000108"
     CDS             complement(523..1668)
                     /gene="garK"
                     /locus_tag="ACHK3A_000108"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO8738263.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1765..2655)
                     /gene="garR"
                     /locus_tag="ACHK3A_000109"
     CDS             complement(1765..2655)
                     /gene="garR"
                     /locus_tag="ACHK3A_000109"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO8738264.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2685..3455)
                     /gene="garL"
                     /locus_tag="ACHK3A_000110"
     CDS             complement(2685..3455)
                     /gene="garL"
                     /locus_tag="ACHK3A_000110"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO8738265.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3471..4805)
                     /gene="garP"
                     /locus_tag="ACHK3A_000111"
     CDS             complement(3471..4805)
                     /gene="garP"
                     /locus_tag="ACHK3A_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO8738266.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5180..6751
                     /gene="garD"
                     /locus_tag="ACHK3A_000112"
     CDS             5180..6751
                     /gene="garD"
                     /locus_tag="ACHK3A_000112"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO8738267.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQGIPLENASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAIP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6900..7235
                     /gene="prlF"
                     /locus_tag="ACHK3A_000113"
     CDS             6900..7235
                     /gene="prlF"
                     /locus_tag="ACHK3A_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO8738268.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7235..7699
                     /gene="yhaV"
                     /locus_tag="ACHK3A_000114"
     CDS             7235..7699
                     /gene="yhaV"
                     /locus_tag="ACHK3A_000114"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO8738269.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(7754..8563)
                     /gene="agaR"
                     /locus_tag="ACHK3A_000115"
     CDS             complement(7754..8563)
                     /gene="agaR"
                     /locus_tag="ACHK3A_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO8738270.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8812..10092
                     /gene="kbaZ"
                     /locus_tag="ACHK3A_000116"
     CDS             8812..10092
                     /gene="kbaZ"
                     /locus_tag="ACHK3A_000116"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO8738271.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCL
                     EHFGEADLVYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10115..10588
                     /gene="agaV"
                     /locus_tag="ACHK3A_000117"
     CDS             10115..10588
                     /gene="agaV"
                     /locus_tag="ACHK3A_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO8738272.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10599..11378
                     /gene="agaW"
                     /locus_tag="ACHK3A_000118"
     CDS             10599..11378
                     /gene="agaW"
                     /locus_tag="ACHK3A_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO8738273.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11368..12246
                     /gene="agaE"
                     /locus_tag="ACHK3A_000119"
     CDS             11368..12246
                     /gene="agaE"
                     /locus_tag="ACHK3A_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO8738274.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12264..12698
                     /gene="agaF"
                     /locus_tag="ACHK3A_000120"
     CDS             12264..12698
                     /gene="agaF"
                     /locus_tag="ACHK3A_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO8738275.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKLGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12695..13828
                     /gene="nagA"
                     /locus_tag="ACHK3A_000121"
     CDS             12695..13828
                     /gene="nagA"
                     /locus_tag="ACHK3A_000121"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO8738276.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAEIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LERIAQRCQRGGPGAQILGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHAQQIWIQGQLASF"
     gene            14178..15332
                     /gene="agaS"
                     /locus_tag="ACHK3A_000122"
     CDS             14178..15332
                     /gene="agaS"
                     /locus_tag="ACHK3A_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO8738277.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15345..16205
                     /gene="kbaY"
                     /locus_tag="ACHK3A_000123"
     CDS             15345..16205
                     /gene="kbaY"
                     /locus_tag="ACHK3A_000123"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO8738278.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16372..16848
                     /gene="agaB"
                     /locus_tag="ACHK3A_000124"
     CDS             16372..16848
                     /gene="agaB"
                     /locus_tag="ACHK3A_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO8738279.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMSITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            16887..17690
                     /gene="agaC"
                     /locus_tag="ACHK3A_000125"
     CDS             16887..17690
                     /gene="agaC"
                     /locus_tag="ACHK3A_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO8738280.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17680..18471
                     /gene="agaD"
                     /locus_tag="ACHK3A_000126"
     CDS             17680..18471
                     /gene="agaD"
                     /locus_tag="ACHK3A_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO8738281.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPLLKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHIGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18472..19239
                     /gene="agaI"
                     /locus_tag="ACHK3A_000127"
     CDS             18472..19239
                     /gene="agaI"
                     /locus_tag="ACHK3A_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO8738282.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(19289..20152)
                     /gene="rsmI"
                     /locus_tag="ACHK3A_000128"
     CDS             complement(19289..20152)
                     /gene="rsmI"
                     /locus_tag="ACHK3A_000128"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO8738283.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            20217..22253
                     /gene="lpoA"
                     /locus_tag="ACHK3A_000129"
     CDS             20217..22253
                     /gene="lpoA"
                     /locus_tag="ACHK3A_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO8738284.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSELTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            22211..22606
                     /gene="yraN"
                     /locus_tag="ACHK3A_000130"
     CDS             22211..22606
                     /gene="yraN"
                     /locus_tag="ACHK3A_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO8738285.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDVWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRITVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            22626..23216
                     /gene="diaA"
                     /locus_tag="ACHK3A_000131"
     CDS             22626..23216
                     /gene="diaA"
                     /locus_tag="ACHK3A_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO8738286.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            23226..23801
                     /gene="dolP"
                     /locus_tag="ACHK3A_000132"
     CDS             23226..23801
                     /gene="dolP"
                     /locus_tag="ACHK3A_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO8738287.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(23914..24954)
                     /gene="yraQ"
                     /locus_tag="ACHK3A_000133"
     CDS             complement(23914..24954)
                     /gene="yraQ"
                     /locus_tag="ACHK3A_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO8738288.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVLLGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(25027..25662)
                     /gene="yraR"
                     /locus_tag="ACHK3A_000134"
     CDS             complement(25027..25662)
                     /gene="yraR"
                     /locus_tag="ACHK3A_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO8738289.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            25790..26308
                     /gene="yhbO"
                     /locus_tag="ACHK3A_000135"
     CDS             25790..26308
                     /gene="yhbO"
                     /locus_tag="ACHK3A_000135"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO8738290.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(26288..26731)
                     /gene="yhbP"
                     /locus_tag="ACHK3A_000136"
     CDS             complement(26288..26731)
                     /gene="yhbP"
                     /locus_tag="ACHK3A_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO8738291.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            26782..27084
                     /gene="yhbQ"
                     /locus_tag="ACHK3A_000137"
     CDS             26782..27084
                     /gene="yhbQ"
                     /locus_tag="ACHK3A_000137"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO8738292.1"
                     /translation="MTPWFLYLIRTADDKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(27071..27574)
                     /gene="yhbS"
                     /locus_tag="ACHK3A_000138"
     CDS             complement(27071..27574)
                     /gene="yhbS"
                     /locus_tag="ACHK3A_000138"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8738293.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(27568..28092)
                     /gene="ubiT"
                     /locus_tag="ACHK3A_000139"
     CDS             complement(27568..28092)
                     /gene="ubiT"
                     /locus_tag="ACHK3A_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO8738294.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            28301..29296
                     /gene="ubiU"
                     /locus_tag="ACHK3A_000140"
     CDS             28301..29296
                     /gene="ubiU"
                     /locus_tag="ACHK3A_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO8738295.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            29305..30183
                     /gene="ubiV"
                     /locus_tag="ACHK3A_000141"
     CDS             29305..30183
                     /gene="ubiV"
                     /locus_tag="ACHK3A_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO8738296.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAILDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            30264..31271
                     /gene="yhbW"
                     /locus_tag="ACHK3A_000142"
     CDS             30264..31271
                     /gene="yhbW"
                     /locus_tag="ACHK3A_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO8738297.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(31389..32633)
                     /gene="mtr"
                     /locus_tag="ACHK3A_000143"
     CDS             complement(31389..32633)
                     /gene="mtr"
                     /locus_tag="ACHK3A_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO8738298.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPTTLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(32787..34676)
                     /gene="deaD"
                     /locus_tag="ACHK3A_000144"
     CDS             complement(32787..34676)
                     /gene="deaD"
                     /locus_tag="ACHK3A_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO8738299.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(34669..34749)
                     /gene="yrbN"
                     /locus_tag="ACHK3A_000145"
     CDS             complement(34669..34749)
                     /gene="yrbN"
                     /locus_tag="ACHK3A_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO8738300.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(34856..35740)
                     /gene="nlpI"
                     /locus_tag="ACHK3A_000146"
     CDS             complement(34856..35740)
                     /gene="nlpI"
                     /locus_tag="ACHK3A_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO8738301.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(35849..37984)
                     /gene="pnp"
                     /locus_tag="ACHK3A_000147"
     CDS             complement(35849..37984)
                     /gene="pnp"
                     /locus_tag="ACHK3A_000147"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO8738302.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(38231..38500)
                     /gene="rpsO"
                     /locus_tag="ACHK3A_000148"
     CDS             complement(38231..38500)
                     /gene="rpsO"
                     /locus_tag="ACHK3A_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO8738303.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(38649..39593)
                     /gene="truB"
                     /locus_tag="ACHK3A_000149"
     CDS             complement(38649..39593)
                     /gene="truB"
                     /locus_tag="ACHK3A_000149"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO8738304.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(39593..39994)
                     /gene="rbfA"
                     /locus_tag="ACHK3A_000150"
     CDS             complement(39593..39994)
                     /gene="rbfA"
                     /locus_tag="ACHK3A_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO8738305.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(40158..42830)
                     /gene="infB"
                     /locus_tag="ACHK3A_000151"
     CDS             complement(40158..42830)
                     /gene="infB"
                     /locus_tag="ACHK3A_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO8738306.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(42855..44342)
                     /gene="nusA"
                     /locus_tag="ACHK3A_000152"
     CDS             complement(42855..44342)
                     /gene="nusA"
                     /locus_tag="ACHK3A_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO8738307.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(44370..44822)
                     /gene="rimP"
                     /locus_tag="ACHK3A_000153"
     CDS             complement(44370..44822)
                     /gene="rimP"
                     /locus_tag="ACHK3A_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO8738308.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(45029..45105)
                     /locus_tag="ACHK3A_000154"
     tRNA            complement(45029..45105)
                     /locus_tag="ACHK3A_000154"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45069..45071),aa:Met,seq:cat)
     gene            45453..46796
                     /gene="argG"
                     /locus_tag="ACHK3A_000155"
     CDS             45453..46796
                     /gene="argG"
                     /locus_tag="ACHK3A_000155"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO8738309.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(46804..48429)
                     /gene="yhbX"
                     /locus_tag="ACHK3A_000156"
     CDS             complement(46804..48429)
                     /gene="yhbX"
                     /locus_tag="ACHK3A_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8738310.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(48886..48972)
                     /locus_tag="ACHK3A_000157"
     tRNA            complement(48886..48972)
                     /locus_tag="ACHK3A_000157"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48936..48938),aa:Leu,seq:gag)
     gene            complement(48987..49319)
                     /gene="secG"
                     /locus_tag="ACHK3A_000158"
     CDS             complement(48987..49319)
                     /gene="secG"
                     /locus_tag="ACHK3A_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO8738311.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQIQPAAPA
                     KPTSDIPN"
     gene            complement(49547..50884)
                     /gene="glmM"
                     /locus_tag="ACHK3A_000159"
     CDS             complement(49547..50884)
                     /gene="glmM"
                     /locus_tag="ACHK3A_000159"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO8738312.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(50877..51725)
                     /gene="folP"
                     /locus_tag="ACHK3A_000160"
     CDS             complement(50877..51725)
                     /gene="folP"
                     /locus_tag="ACHK3A_000160"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO8738313.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYHDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(51815..53749)
                     /gene="ftsH"
                     /locus_tag="ACHK3A_000161"
     CDS             complement(51815..53749)
                     /gene="ftsH"
                     /locus_tag="ACHK3A_000161"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO8738314.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(53849..54478)
                     /gene="rlmE"
                     /locus_tag="ACHK3A_000162"
     CDS             complement(53849..54478)
                     /gene="rlmE"
                     /locus_tag="ACHK3A_000162"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO8738315.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            54604..54897
                     /gene="yhbY"
                     /locus_tag="ACHK3A_000163"
     CDS             54604..54897
                     /gene="yhbY"
                     /locus_tag="ACHK3A_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO8738316.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(55053..55529)
                     /gene="greA"
                     /locus_tag="ACHK3A_000164"
     CDS             complement(55053..55529)
                     /gene="greA"
                     /locus_tag="ACHK3A_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO8738317.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            55777..57210
                     /gene="dacB"
                     /locus_tag="ACHK3A_000165"
     CDS             55777..57210
                     /gene="dacB"
                     /locus_tag="ACHK3A_000165"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO8738318.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(57251..58423)
                     /gene="cgtA"
                     /locus_tag="ACHK3A_000166"
     CDS             complement(57251..58423)
                     /gene="cgtA"
                     /locus_tag="ACHK3A_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO8738319.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(58439..59404)
                     /gene="yhbE"
                     /locus_tag="ACHK3A_000167"
     CDS             complement(58439..59404)
                     /gene="yhbE"
                     /locus_tag="ACHK3A_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8738320.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLGPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(59531..59788)
                     /gene="rpmA"
                     /locus_tag="ACHK3A_000168"
     CDS             complement(59531..59788)
                     /gene="rpmA"
                     /locus_tag="ACHK3A_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO8738321.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(59809..60120)
                     /gene="rplU"
                     /locus_tag="ACHK3A_000169"
     CDS             complement(59809..60120)
                     /gene="rplU"
                     /locus_tag="ACHK3A_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO8738322.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVTIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            60379..61350
                     /gene="ispB"
                     /locus_tag="ACHK3A_000170"
     CDS             60379..61350
                     /gene="ispB"
                     /locus_tag="ACHK3A_000170"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO8738323.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            61578..61856
                     /gene="sfsB"
                     /locus_tag="ACHK3A_000171"
     CDS             61578..61856
                     /gene="sfsB"
                     /locus_tag="ACHK3A_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO8738324.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(61904..63163)
                     /gene="murA"
                     /locus_tag="ACHK3A_000172"
     CDS             complement(61904..63163)
                     /gene="murA"
                     /locus_tag="ACHK3A_000172"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO8738325.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(63218..63472)
                     /gene="ibaG"
                     /locus_tag="ACHK3A_000173"
     CDS             complement(63218..63472)
                     /gene="ibaG"
                     /locus_tag="ACHK3A_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO8738326.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(63632..63925)
                     /gene="mlaB"
                     /locus_tag="ACHK3A_000174"
     CDS             complement(63632..63925)
                     /gene="mlaB"
                     /locus_tag="ACHK3A_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO8738327.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(63925..64560)
                     /gene="mlaC"
                     /locus_tag="ACHK3A_000175"
     CDS             complement(63925..64560)
                     /gene="mlaC"
                     /locus_tag="ACHK3A_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO8738328.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(64579..65130)
                     /gene="mlaD"
                     /locus_tag="ACHK3A_000176"
     CDS             complement(64579..65130)
                     /gene="mlaD"
                     /locus_tag="ACHK3A_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO8738329.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(65135..65917)
                     /gene="mlaE"
                     /locus_tag="ACHK3A_000177"
     CDS             complement(65135..65917)
                     /gene="mlaE"
                     /locus_tag="ACHK3A_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO8738330.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(65925..66734)
                     /gene="mlaF"
                     /locus_tag="ACHK3A_000178"
     CDS             complement(65925..66734)
                     /gene="mlaF"
                     /locus_tag="ACHK3A_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO8738331.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            66944..67921
                     /gene="yrbG"
                     /locus_tag="ACHK3A_000179"
     CDS             66944..67921
                     /gene="yrbG"
                     /locus_tag="ACHK3A_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO8738332.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            67935..68921
                     /gene="kdsD"
                     /locus_tag="ACHK3A_000180"
     CDS             67935..68921
                     /gene="kdsD"
                     /locus_tag="ACHK3A_000180"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO8738333.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGVTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            68942..69508
                     /gene="kdsC"
                     /locus_tag="ACHK3A_000181"
     CDS             68942..69508
                     /gene="kdsC"
                     /locus_tag="ACHK3A_000181"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO8738334.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSIAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            69505..70080
                     /gene="lptC"
                     /locus_tag="ACHK3A_000182"
     CDS             69505..70080
                     /gene="lptC"
                     /locus_tag="ACHK3A_000182"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO8738335.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            70049..70606
                     /gene="lptA"
                     /locus_tag="ACHK3A_000183"
     CDS             70049..70606
                     /gene="lptA"
                     /locus_tag="ACHK3A_000183"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO8738336.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            70613..71338
                     /gene="lptB"
                     /locus_tag="ACHK3A_000184"
     CDS             70613..71338
                     /gene="lptB"
                     /locus_tag="ACHK3A_000184"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO8738337.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            71386..72819
                     /gene="rpoN"
                     /locus_tag="ACHK3A_000185"
     CDS             71386..72819
                     /gene="rpoN"
                     /locus_tag="ACHK3A_000185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO8738338.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            72842..73129
                     /gene="hpf"
                     /locus_tag="ACHK3A_000186"
     CDS             72842..73129
                     /gene="hpf"
                     /locus_tag="ACHK3A_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO8738339.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            73247..73738
                     /gene="ptsN"
                     /locus_tag="ACHK3A_000187"
     CDS             73247..73738
                     /gene="ptsN"
                     /locus_tag="ACHK3A_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO8738340.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            73784..74638
                     /gene="rapZ"
                     /locus_tag="ACHK3A_000188"
     CDS             73784..74638
                     /gene="rapZ"
                     /locus_tag="ACHK3A_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO8738341.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            74635..74907
                     /gene="npr"
                     /locus_tag="ACHK3A_000189"
     CDS             74635..74907
                     /gene="npr"
                     /locus_tag="ACHK3A_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO8738342.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            75120..75752
                     /gene="yrbL"
                     /locus_tag="ACHK3A_000190"
     CDS             75120..75752
                     /gene="yrbL"
                     /locus_tag="ACHK3A_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO8738343.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(75749..76477)
                     /gene="mtgA"
                     /locus_tag="ACHK3A_000191"
     CDS             complement(75749..76477)
                     /gene="mtgA"
                     /locus_tag="ACHK3A_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO8738344.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(76474..77127)
                     /gene="elbB"
                     /locus_tag="ACHK3A_000192"
     CDS             complement(76474..77127)
                     /gene="elbB"
                     /locus_tag="ACHK3A_000192"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO8738345.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(77357..79693)
                     /gene="arcB"
                     /locus_tag="ACHK3A_000193"
     CDS             complement(77357..79693)
                     /gene="arcB"
                     /locus_tag="ACHK3A_000193"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO8738346.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLALVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(79789..80718)
                     /gene="yhcC"
                     /locus_tag="ACHK3A_000194"
     CDS             complement(79789..80718)
                     /gene="yhcC"
                     /locus_tag="ACHK3A_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO8738347.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQTECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            81393..85853
                     /gene="gltB"
                     /locus_tag="ACHK3A_000195"
     CDS             81393..85853
                     /gene="gltB"
                     /locus_tag="ACHK3A_000195"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO8738348.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            85866..87284
                     /gene="gltD"
                     /locus_tag="ACHK3A_000196"
     CDS             85866..87284
                     /gene="gltD"
                     /locus_tag="ACHK3A_000196"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO8738349.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDLSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            87467..88594
                     /gene="yhcG"
                     /locus_tag="ACHK3A_000197"
     CDS             87467..88594
                     /gene="yhcG"
                     /locus_tag="ACHK3A_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO8738350.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNPDARSFYE
                     KETLRCGWSVRQLERQITTQFYERTLLSHDKSAMLQQHAPAEMHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(88654..89118)
                     /gene="nanQ"
                     /locus_tag="ACHK3A_000198"
     CDS             complement(88654..89118)
                     /gene="nanQ"
                     /locus_tag="ACHK3A_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO8738351.1"
                     /translation="MIMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQCDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(89115..89990)
                     /gene="nanK"
                     /locus_tag="ACHK3A_000199"
     CDS             complement(89115..89990)
                     /gene="nanK"
                     /locus_tag="ACHK3A_000199"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO8738352.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALHDA
                     LSALVSPLQVHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDITEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHCSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(89987..90676)
                     /gene="nanE"
                     /locus_tag="ACHK3A_000200"
     CDS             complement(89987..90676)
                     /gene="nanE"
                     /locus_tag="ACHK3A_000200"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO8738353.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(90724..92214)
                     /gene="nanT"
                     /locus_tag="ACHK3A_000201"
     CDS             complement(90724..92214)
                     /gene="nanT"
                     /locus_tag="ACHK3A_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO8738354.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(92324..93217)
                     /gene="nanA"
                     /locus_tag="ACHK3A_000202"
     CDS             complement(92324..93217)
                     /gene="nanA"
                     /locus_tag="ACHK3A_000202"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO8738355.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(93339..94130)
                     /gene="nanR"
                     /locus_tag="ACHK3A_000203"
     CDS             complement(93339..94130)
                     /gene="nanR"
                     /locus_tag="ACHK3A_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO8738356.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            94510..95877
                     /gene="dcuC"
                     /locus_tag="ACHK3A_000204"
     CDS             94510..95877
                     /gene="dcuC"
                     /locus_tag="ACHK3A_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO8738357.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCIIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTLFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAIGAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(95921..96418)
                     /gene="sspB"
                     /locus_tag="ACHK3A_000205"
     CDS             complement(95921..96418)
                     /gene="sspB"
                     /locus_tag="ACHK3A_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO8738358.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(96424..97062)
                     /gene="sspA"
                     /locus_tag="ACHK3A_000206"
     CDS             complement(96424..97062)
                     /gene="sspA"
                     /locus_tag="ACHK3A_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO8738359.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(97457..97849)
                     /gene="rpsI"
                     /locus_tag="ACHK3A_000207"
     CDS             complement(97457..97849)
                     /gene="rpsI"
                     /locus_tag="ACHK3A_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO8738360.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(97873..98301)
                     /gene="rplM"
                     /locus_tag="ACHK3A_000208"
     CDS             complement(97873..98301)
                     /gene="rplM"
                     /locus_tag="ACHK3A_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO8738361.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(98520..99647)
                     /gene="zapE"
                     /locus_tag="ACHK3A_000209"
     CDS             complement(98520..99647)
                     /gene="zapE"
                     /locus_tag="ACHK3A_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO8738362.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYDIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            99841..100239
                     /gene="zapG"
                     /locus_tag="ACHK3A_000210"
     CDS             99841..100239
                     /gene="zapG"
                     /locus_tag="ACHK3A_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO8738363.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            100393..101760
                     /gene="degQ"
                     /locus_tag="ACHK3A_000211"
     CDS             100393..101760
                     /gene="degQ"
                     /locus_tag="ACHK3A_000211"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO8738364.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            101850..102917
                     /gene="degS"
                     /locus_tag="ACHK3A_000212"
     CDS             101850..102917
                     /gene="degS"
                     /locus_tag="ACHK3A_000212"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO8738365.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(102980..103918)
                     /gene="mdh"
                     /locus_tag="ACHK3A_000213"
     CDS             complement(102980..103918)
                     /gene="mdh"
                     /locus_tag="ACHK3A_000213"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO8738366.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            104353..104823
                     /gene="argR"
                     /locus_tag="ACHK3A_000214"
     CDS             104353..104823
                     /gene="argR"
                     /locus_tag="ACHK3A_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO8738367.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        25557 a        28176 c        26310 g        24884 t
ORIGIN      
        1 atgcaatata gacttaataa cttcttacaa agtctattat taataccgta gatattcatc
       61 ttaccctggg caccaaaagc aaaaacccgc cgaagcgggt ttttacgtaa atcaggtgaa
      121 actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag caatcgctca
      181 ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc cctatttggc
      241 cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg tgcgctctta
      301 ccgcaccctt tcacccttac ctgatcccgc ttacgcgggc catcggcggt ttgctctctg
      361 ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct atggagcccg
      421 gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg gcgactgtct
      481 ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg cgttgcgcat
      541 tccaatcgcc agtgtcgcgg cgatattacg tgaagcacgg cagatattgt cataagcccc
      601 gcggaatgct tcgtccaacg taccaatgct ggtcaatacg ctgaagaccg catcaatgcc
      661 atgctgatgt acaacgccaa catcattggt caggctaccc gcaatgccaa tcaccggttt
      721 atggtacttc ttcgccacgt ttgcgacacc aatcggtacc ttgccgtgaa tactctggct
      781 atcaatacgc ccttcaccag tgatcaccag cgtacaatcg tgaatatgtt cctccagatt
      841 cagcgccgta gtgacgattt caataccact tttcagttct gcaccaagaa acgccatcag
      901 cgccgcgccc ataccgcccg cagctcctgc gccggggaca tctttcacat caacatgcag
      961 cgctttttta ataacatcgg catagtgaga gaggttattg tccagctcga caatcatcgc
     1021 ctcactggct cccttttgtg ggccaaagat gcgcgaagca ccactgtcgc cgactaacgg
     1081 gttggtgaca tcacaagcga cgcggatgac acaatctttt aagcgcggat cgagaccgga
     1141 aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt cattgccgtt
     1201 ggcatcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccgt cgttggtggc
     1261 gctgccgcca atgccgataa taatgtttgt cgcaccgctc tccagcgcct gcaggattaa
     1321 ctcaccagta ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag gtaccagttc
     1381 cagcccactg gccgccgcca tttcgataaa cgcggttttg ccatcgccgg agatccccca
     1441 actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt cagccccctg
     1501 ggtggctgca atcatcgctt ccaccgttcc ttcaccaccg tcggcaaccg gaacagaaac
     1561 atactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg caacctcgct
     1621 ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac ctatgcctgt
     1681 taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc ccggattgca
     1741 atacgccatc cgggcacgac gtcattagcg agtaacttcg actttcgcca gtttttcgta
     1801 gtagcacgcc agggcgctat gatccgccgt tcctaaacca tctgcacgca gtgcctgcat
     1861 catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtgag aagtatccag
     1921 cgcattcgcc agatccttaa tatgcagatc aatacggaag cccggtttga agttgcggtc
     1981 catcaccatc ggcgctttgg catccagcac ggtactgccc gccagcccac cgcgaattgc
     2041 ctgatacacc agatccgggt taacgcccgc tttggttgcc aacgttaacg cttctgacat
     2101 cgcagcaata ttcagcgcca caatgacctg atttgccagt ttggtgacgt tacctgcacc
     2161 gatttccccg gtatgcacca cggaacccgc catcgctttc atcaaatcat agtatttgtc
     2221 gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg ctttcggttc
     2281 accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg cttcgctgat
     2341 ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac ctggcttcgc
     2401 gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg agtttggcag
     2461 catggttatg attacgtcgc actgttcagc gatcgcttta gccgtagagg ctgtttctgc
     2521 acctgcagca atcacgtcag cgatagcttc tgggttacgg tcagcaacca ccagcgagta
     2581 acctgctttc aaaaggtttt tactcattgg tttacccata atcccaaggc caataaaacc
     2641 aactttcata gtcatatcaa tcatctctct tattgcggtg gtggttattt tttaaaggta
     2701 tcagccagtt tctgagtggc agagcggaag acgccgagat cgctgccgac ggccacaaac
     2761 gtcgcgcccc attccagata acgacgcgca tcagcctcga ctggtgcgag gatgccgctg
     2821 ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg tacatccggg
     2881 tgcgaggcat tgccgagatg gcctaatgcc gcagccagat cgctggggcc gacgaagatg
     2941 ccgtctacac cttcggtagc ggcaatagca tcgacgttat ctacgccctg ctgactttct
     3001 atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc cacggtgcca
     3061 aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg gtaacgagtt
     3121 gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag gaagttatag
     3181 aaaccgatat ccagaagacg cttaataatt accggctcgt tggtcggcac tcgcactact
     3241 ggcgcgctgg cgctgccttt cagggccatt aactgcggaa taaacgtgga aatatcgttt
     3301 ggcgcatgtt caccatccag caccagccag tcaaacccag ccaaaccaag aacttcagtg
     3361 ctaatcgggt tagagagagc tgaccagcaa ccaatttgta cctgtttcgc agccagtgcg
     3421 gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc ttatttctgc
     3481 aattccatac gtttaatgtc gccgactacg aagaggtagc agaccatcgc catcagcgct
     3541 gaacatccca caaaaaccag tgctgcattg aaggagtgca gttcacttac caggtagcca
     3601 atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac gccgccgcag
     3661 aggccaacaa tctctttcgg cgcggtgtca gaaatcactg gccagcccag cgcaccaaat
     3721 cctttaccaa agaaagccag cgccatcagc atgaccacca gcgtggtgtt gttggtgtag
     3781 ttacataaga tgatggtgga agccagcaac attcccagca caattggtag cttacgtgcc
     3841 agggtcaggg acaaaccgcg tttgatcaga taatccgaga agacacctcc cagcacgccg
     3901 cccgcaaagc cacacagtgc cggaatcgag gcgaccagac ccactttcag aatcgacatg
     3961 cctttttcct gcaccagata aattgggaac caggtgagga agaaccaggt gatggtgttg
     4021 ataaaatatt gtccgaaaaa tacgcccagc atcatgcggt tagagagcaa ttgcttgatg
     4081 taatgcagtt tgggtccgct tgctgccgca ctgcccggct ttttgtggtc catatcgacc
     4141 accgcgccat tttccgagat aaacttcagc tcttccgcag acatacgtgg gtgatctgtc
     4201 gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aaccaattac ccccataacg
     4261 gtaaagacgt gctcccagcc ccacgcgaaa gtcagccagc caagcagcgg cgaaaagagc
     4321 gccagcgaga aatattgcgc cgagttaaag atggcggagg cagtaccacg ttctttcgtc
     4381 gggaaccagg cggcgacaat acgggcgttc gccgggaatg atggcgcttc cgagaacccc
     4441 agcataaagc gcataaagaa catggagatc cctgcccagg ccagcgggaa catatcaaca
     4501 aagccttgca ggaaggtgaa tagcgaccag aaaaagaggc tgtaggtgta aacttttttc
     4561 gaaccaaact tatcaagcag ccagccgccg gggatttgca tcagcaagta ggcccagcca
     4621 aaagcagaga agatgtaacc catcgaaacc gcacttaact gcaactcttt tgccacttcg
     4681 gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac aataaaaata
     4741 atcagtaata ttaaatagcg ggtatgcacg cctttctttt tttcgtcaac ggtgtccagc
     4801 atcattttat ttacctcggg tacttatgct gatttttatt attatgggga aggtgttatt
     4861 tatgagtttc atttatgccg ttacgacaat gaactcggga attagtataa gcagcgcgag
     4921 aataataatc attgtgcaaa tgctaattta attagtacta tttaaatatt attttgagca
     4981 tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc gggcggcact
     5041 gcaatccctg aaatgattga cattgatcac atttctgcat ttaaactcct gacattctta
     5101 tttcacccaa tgaagttatt tatttttaaa tgagaccagg tcctcatttt aataacccct
     5161 ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc caactgcgtt
     5221 ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata atggcctgaa
     5281 agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc aggggcataa
     5341 agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg aagtgattgg
     5401 ttacgccgtg cgcgcaatcc cacgcggaag ctggatcgac gaatcaatgg ttgtactacc
     5461 ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggcac ccttaccgcc
     5521 actggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg gcaccaaaaa
     5581 cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact atgtagtaaa
     5641 aatcattgaa cgcgatctgc tgccgaaata cccgaacgtc gatggcgtgg tggggctgaa
     5701 tcatctgtac ggttgtggcg tggcgattaa cgctccggcg gcggtggtgc ccattcggac
     5761 cattcacaac atttcgctta acccaaactt tggcggcgag gtgatggtga ttggcctggg
     5821 ttgcgagaaa ttacagccag aacgcctgct ggtcggcacg gatgatgtac aaggtattcc
     5881 gctggagaac gccagtattg tcagcttgca ggatgaaaag catgtcggtt ttcagtccat
     5941 ggtcgaggat attttgcagg tcgccgaacg ccatttgcac aaactgaatc aacggcagcg
     6001 agaaacctgt ccagcttcag aactggttgt cggcatgcag tgcggtggca gcgatgcgtt
     6061 ttctggcgta acggcaaacc cggcggttgg gtatgcgtct gatctactgg tgcgctgcgg
     6121 cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc tgctgacacc
     6181 acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt ggtacgataa
     6241 ctatctcaat atgggaaaaa ccgaccgtag cgccaaccct tcgccgggca acaagaaagg
     6301 cggtctggca aatgtggtgg agaaagcgct cggctccatt gctaaatcgg gtaaaagcgc
     6361 aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa tttacgccgc
     6421 gacgccagcc agcgattttg tctgtggcac acaacaggtg gcttcaggga ttaccgtgca
     6481 agtgtttacg accggtcgcg gtacgccgta cggcctgatg gcgatacccg tcattaaaat
     6541 ggcgacccgc accgagctgg cgaaccgctg gtttgattta atggatatta acgcgggcac
     6601 tatcgctacc ggcgaagaaa ccattgaaga agtgggctgg aagttgttcc actttattct
     6661 cgacgtcgcc agcgggaaga agaaaacttt ctcggatcaa tggggactgc ataaccagct
     6721 ggcggtgttt aacccggcac cagtgacctg atttcttttg gaacgtccct cgcaaaacat
     6781 ggccttagtg ccatgttttt attgtttaaa gtccacacat ccattaataa tgcatttgca
     6841 ttaccttagt tcaagcttat aatttgagca gaaaacaggc tgtaaaagga cagtaaataa
     6901 tgcccgctaa tgctcgctct aacgctgtac tgaccactga atcaaaggtc actatacgcg
     6961 gacaaacaac tatccccgcg ccagtgcgtg aggccttaaa actgaagcca ggcctggaca
     7021 gcattcatta cgaaattctg cctggtgggc aagtatttat gtgccgactg ggagatgaac
     7081 aggaggatca tactatgaat gcatttttgc gttttctgga tgcagatatc cagaacaacc
     7141 cgcaaaaaac tcgtccattc gacattcaac aaggaaagaa acttgtcgct ggcatggacg
     7201 tcaacattga cgatgagatt ggcgacgacg aataatggat tttccacaaa gggttaatgg
     7261 ttgggcgcta tatgctcatc cctgttttca ggaaacctac gatgctttag ttgccgaagt
     7321 cgaggcatta aagggaaaag atcctgaaaa ttatcagaga aaagctgcca caaagttatt
     7381 ggcggtggtc cataaagtga ttgaggagca tatcacggtc aatccatcat caccggcatt
     7441 ccgtcatggc aagtcgttag gctctgggaa aaataaagac tggtcacggg taaaatttgg
     7501 tgctggtcgc tatcgtctct tctttcgtta cagtgaaaaa gagaaagtca tcattctggg
     7561 atggatgaac gatgaaaaca cgctgcgcac ctacggtaaa aaaacagatg cctataccgt
     7621 attcagtaaa atgttaaaaa gaggacatcc tcctgccgac tgggaaagcc tcacccaaga
     7681 gacagaagaa aaccattgat ggtgttcaca atgcccgccg aacgcattgg tgttgaccag
     7741 cgggcatcct ttctcattcc ccgaccagaa tcacttcaac cccagccttt ctcagtcctt
     7801 ccagactatc cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa
     7861 tgatcttatg taaactagaa cgattgaact tactggaatc ggtgaccacg atgatccgtt
     7921 ccgcaacttc gcacatccga cggtttaaac gggcttcatc ttcattatgc gtgctgacgc
     7981 cgcgctccag atcgatcgca tctacaccaa gaaacagcat atcgaagtgg taattttgca
     8041 gcgattgctc agcctgatca ccgtaaaaag attgcgactg acggcgcaaa tgccccccgg
     8101 tcatcagcag ctcaacgcct tccgcttcca gcaacgcatt agccacgttc ataccgttgg
     8161 tcatcgcaat tacgtcggtg tgcttgcgca tcagacgagc aatctcaaaa gtggtggtcc
     8221 cggagtcgag gatcacccga tgaccaggct gaatcaactc aacggcagct ttcgcaacgc
     8281 tgcgtttcat cgcggtgttc agcgcgcttt tatcttccac tgatggctcg actgacggcg
     8341 ttgtgctatc gcagatcaac gcgccaccat aggcacgcac agcgatcccc tgcttttcca
     8401 gaaacgccag atcgttgcgg atcgtcacag tagatacacc atacaacgcc gatagatcat
     8461 taacttgcac acttccttgc tggcgcaggc gttgaatgat ctgctctcgt cgctcgctgg
     8521 tgcctgtcac tcgcttctca cctgaagcgt cggtattact catagtaagt cctttcgtaa
     8581 aactttcgtt tcatttcgtt ttgcctatta acgcctttct attaagcaaa tgcaagccca
     8641 ccttacccat tggcgcaagc tactctcgtt tcagtggctt tcattatgtt tcttttgtga
     8701 atcagatcag aaaaccatta tctttcgttt tatttttatc tcaccatgac gcagtatcaa
     8761 ctgaaacaaa acgaaagatt aatgtcgcag taatctgaac tggagaggaa agtgaaacat
     8821 ctgacagaaa tggtgagaca gcacaaagcg ggcaaaacaa atgggattta tgccgtttgt
     8881 tccgcacatc cgctggtgct ggaagctgca atccgctacg ccagtgcaaa ccaaacgccg
     8941 ttactgattg aagcaacctc caatcaggtg gaccagttcg gcggttatac cggaatgacg
     9001 cccgccgatt ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt cccgcaggat
     9061 gcgctgattc tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct gcctgccgct
     9121 caggcaatgg ccaatgccga tgatttgatt aaaagctacg tggcggcagg attcaaaaaa
     9181 attcaccttg attgcagcat gtcctgtcag gacgatccga ttcccttaac cgatggcatc
     9241 gtggccgaac gcgccgcccg tctggcgaaa gtggcggaag aaacctgtct tgaacacttt
     9301 ggcgaagccg atctggtgta tgtcattggc accgaagtac cggtacctgg cggcgcgcat
     9361 gaaaccttaa gcgagctggc ggtcaccacg ccggatgccg cccgcgccac gctggaagcc
     9421 catcgtcacg cctttgaaaa gcaaggtctg agtgccatct ggccacgcat cattgctctg
     9481 gtggtacaac ccggcgtcga attcgatcac accaacgtta ttgattatca gcccgtcaaa
     9541 gcgaccgcct taagccagat ggtcgaaaac tacgaaacgc tgattttcga agcgcactct
     9601 accgattacc aaacgccgca atcgttgcgc cagctagtga ttgaccactt tgccatcctg
     9661 aaagttggcc cggcgctgac cttcgccctg cgtgaagctc tgttctctct ggcggcgatt
     9721 gaagaagaac tggtaccagc aaaagcctgt tctggtctgc gtcaggtgct ggaaaacgtg
     9781 atgctcgacc gcccggaata ctggcaaagc cactaccacg gtgacggcaa cgcgcgtcgt
     9841 ctggcgcgtg gttatagcta ctcggatcgc gtgcgctatt actggccgga cagccagatt
     9901 gatgacgctt tcgctcatct ggttcgtaat ctggcggatt caccaattcc gctgccgctg
     9961 atcagtcagt atctgccgct gcagtacgtg aaagttcgct ccggcgagct gcagccaacg
    10021 ccacgggaac tcattatcaa ccatattcag gacatcctgg cgcagtacca cacagcctgt
    10081 gaaggccaat aagcaaaaca aagaggaaca cgctatgcca aatattgttt taagtcggat
    10141 tgatgaacgc ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg cgggggcaaa
    10201 tctggtgctg gtagccaacg atgaggttgc cgaagatccg gtacaacaaa acctgatgga
    10261 aatggtactg gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag tcatcgacaa
    10321 cattcatcgc gccgccgatc gacagaaaat cctgctagtt tgtaaaacac cggccgattt
    10381 cctgacgttg gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca atatgcacta
    10441 cgccaatggc aagcaacaaa tcgccaaaac ggtttctgtg gatgcgggtg atatcgcagc
    10501 atttaacgat ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc
    10561 tgctgtagac ctctttaaat tactttgagg gattcatcat ggaaatcagt ctgttgcagg
    10621 catttgcgtt gggaattatc gcctttatcg ctggcctgga tatgtttaac ggcttaaccc
    10681 atatgcaccg cccggtggtt ctcggcccgt tggtcgggct ggtacttggc gatctgcata
    10741 ccggtatttt aaccggcggt acgctggaac tggtgtggat ggggctggcc cctctggcgg
    10801 gcgcacagcc gcctaacgtg attatcggta ctatcgtcgg cacggcgttt gccattacta
    10861 ctggcgtgaa acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc gcagtacaga
    10921 tggggattac cttcctgttc tcggtgatgt ccggcgtgat gtcccgatgc gaccggatgg
    10981 cggagaacgc cgatactcgc ggcattgaac gcgtgaacta tctggcattg ctggcactcg
    11041 gcacgttcta ttttctctgc gctttcctgc ctatctactt cggcgcggaa catgcaaaaa
    11101 ccatcattga tgtcctgccg caacgattaa ttgatggcct cggcgtcgcg ggcggcatca
    11161 tgccagcaat cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc tacatcccct
    11221 acttcatcct gggctttgtt gccgcggcct ggctcaagtt accggtgctg gctattgctg
    11281 ccgctgcgct ggcgatggcg ctgatcgacc tgctgcgtaa atctcctgaa ccgactcaac
    11341 ctgcggcaca gaaagaggaa tttgaagatg gcatctaatc aaaccaccct gccgaacgtc
    11401 agcgaaaatg aagaaacact gctgactggc gtcaatgaaa acgtgtatga agatcagagc
    11461 attggcgcgg agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc catgctgtta
    11521 caggcttctt tcaactacga acgtatgcag gcttccggct ggctgtacgg cctgctgcca
    11581 gcactgaaaa agatccacac taataaacgc gacctggcgc gcgccatgaa ggggcatatg
    11641 ggtttcttca atacccatcc gtttctggtg acgtttgtta tcggcattat ccttgcgatg
    11701 gagcgttcta agcaggacgt taacagtatt cagagcacca aaattgccgt cggtgcgccg
    11761 ctcggcggga ttggcgatgc gatgttctgg ctaacgctac tgccgatttg tggcgggata
    11821 ggtgcaagcc tcgcgctaca aggctctatt cttggcgctg tcgtctttat tgtgctgttc
    11881 aacgtggtgc acctggggct gcgttttggt ctggcgcatt atgcttaccg catgggcgtg
    11941 gcggcgattc cactcattaa agcaaatacc aaaaaagtcg gccatgcggc atctatcgtt
    12001 gggatgacgg taatcggcgc gctggtggca acctatgttc gtttaagcac cacgctggaa
    12061 atcaccgcgg gcgacgcagt ggttaagtta caggctgatg ttatcgacaa actgatgcca
    12121 gccttcttac cgctggtcta caccctgacc atgttctggc tggtacgccg cggctggagt
    12181 ccgctgcgcc tgattgcagt gaccgtggtt ctcggcatcg tcggtaaatt ctgccatttc
    12241 ctttaaatac aatgaggtat tagatgttaa gtattatttt gacagggcat ggcgggtttg
    12301 ccagtggcat ggaaaaagca atgaagcaaa tcctcggcga gcaatcgcag tttattgcca
    12361 tcgattttcc ggaaacctca agcaccgcgc tgcttacatc gcagcttgaa gaggccatcg
    12421 cccaactgga ttgtgaagac ggcattgttt ttctgacgga tttgctgggc ggcacgccgt
    12481 ttcgcgtagc ttcaacgctg gcgatgcaaa aactgggctg tgaagtgatt accggcacca
    12541 atttgcaact gctactggag atggtgctgg agcgcgaagg gttaagcggt gaagagtttc
    12601 gtgtgcaggc gctggagtgt ggacatcgtg ggctgaccag tctggtggac gagttaggcc
    12661 gctgccatga ggaatgtccg gtcgaggaag gaatatgaca cccgttttgc gcgccagaag
    12721 gctgctgact gaagagggat ggctcgatga ccatcagttg cgtattgctg acggtgtaat
    12781 cgcagaaatc gaaccgattc cagtgggcgt gactgaacgc gatgcggaac tgctctgccc
    12841 cgcttacatc gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg atgacgcgcc
    12901 ggatgtgctc gacaagctgg caatgcacaa agcacgcgaa ggtgtcggca gttggttacc
    12961 gaccaccgta accgcgccgc ttaataccat tcatgcggcg ctggaacgta ttgctcaacg
    13021 ttgccaacgc ggcggacctg gtgcacaaat actggggagt tatctcgaag gaccgtactt
    13081 cacgccgcag aataaaggcg cgcatccgcc ggagctgttt cgcgagctgg aaattgccga
    13141 gctggatcaa ttgattgccg tttctcagca caccttgcgc gtggtggcgc tggcaccgga
    13201 aaaagaaggg gcattgcagg ccatccgcca tcttaaacag caaaacgtaa gggtgatgct
    13261 ggggcatagc gcggcgacct ggcaacaaac ccgcgccgcg tttgatgctg gtgccgacgg
    13321 cctggtgcat tgctataacg ggatgacagg tttacatcac cgcgaaccgg gaatggttgg
    13381 cgcgggatta acggacaagc gcgcctggct ggaactgata gccgatggtc atcatgtgca
    13441 tccggcggcg atgtcgctgt gttgttgctg tgcgaaagag agaatcgtgc tcatcaccga
    13501 cgcgatgcag gcagccggga tgccggatgg tcgctatacg ttatgtggcg aagaagtgca
    13561 gatgcacggt ggcgttgtcc gtaccgcgtc cggtggactg gcgggcagta cgctgtctgt
    13621 cgatgcggca gtgcgcaaca tggtcgagtt gacgggcgta acgcctgcgg aagccatcca
    13681 tatggcgtcg ctgcatccgg cgcgtatgct gggtgttgat gatgttctgg gatcgcttaa
    13741 accgggcaaa cgcgccagcg tcgttgcgct ggatagcggg ctgcatgcgc aacaaatctg
    13801 gattcagggt caattagctt cgttttgatg gtttgctcct ttattgggcc ttcacttccc
    13861 ccgtaaggcc tttctttttc tttcgttttg atctgtgcag cggtgtcgga tgcgacgcta
    13921 atgcgcctta tccgacctac ggttggtgac cgtaaggccg gataaagtat ttacgccgca
    13981 tccagcaatc ccttttactt cccttgtctt ttctttcaac gatcacagat ttcgttttat
    14041 ttcctttttc tccattgaac tttcagtttc ttttctatag attttaacca acgaaagaaa
    14101 tcaccaagtg aaatgaaacg aaaggcaaac gaaagcgaca acgccgacgt caagttcatc
    14161 agactaagga ttgagttatg ccagaaaatt acacccctgc tgctgccgca accggtacat
    14221 ggactgaaga agagatccgc catcagcctc gcgcatggat ccgttcactc accaacatcg
    14281 acgcgctacg ttccgcgctc aataacttcc ttgaaccgtt actgcgcaaa gagaatctgc
    14341 aaatcatcct gaccggagcc ggaacatcgg catttatcgg tgacatcatc gcgccgtggc
    14401 tcgccagtca taccggtaaa aacttcagcg ccgtaccgac caccgatctg gtcactaatc
    14461 cgatggacta cctgaatcca gctcatccgc tgctgttgat ctccttcggt cgatccggca
    14521 acagcccgga aagcgtcgcc gccgtggaac tggcaaatca atttgtaccc gagtgctatc
    14581 acctgccgat cacctgcaac gaagcgggcg ctctttacca aaacgcgatc aacagcgaca
    14641 acgcgtttgc cctactgatg cccgcagaaa cgcacgaccg cggctttgcg atgaccagca
    14701 gcattaccac catgatggcc agttgcctcg cggttttcgc acctgagacg atcaacagcc
    14761 agagcttccg cgacgtggcg gatcgttgcc aggcgatcct gacctcactg ggcgatttca
    14821 gcgaaggtgt gtttggttac gcaccgtgga aacggatcgt ttatctcggc agcggtggct
    14881 tacagggcgc agcacgcgag tcggcgctga aagtgctgga actgacagcg ggtaaactgg
    14941 cggccttcta tgattccccg accggattcc gtcatggccc gaaatcgctg gtcgataacg
    15001 aaacgctggt ggtggtattt gtctccagcc acccttacac ccgtcagtat gatcttgatc
    15061 tgctggcaga actccgccgc gataaccagg caatgcgcgt aatcgccatc gccgcggaaa
    15121 gcaatgacgt tattactgcc ggtccacata tcatcctgcc gccgtcccgt cactttatcg
    15181 acgttgagca ggcattttgc ttcctgatgt acgcccagac atttgcactg atgcagtcgc
    15241 tgcacatggg taatacaccg gataccccat cagccagtgg caccgttaac cgcgtggtgc
    15301 aaggcgtaat cattcatccg tggcaggcat aagaggatcg cattatgagc attatctcca
    15361 ccaaatatct gttacaggac gcccaggcca atggctacgc ggtgcctgcc tttaacattc
    15421 acaacgccga gacgatccag gcgatcctcg aagtgtgcag tgaaatgcga tcaccggtga
    15481 tcctcgccgg gacgccgggg acctttaagc atatcgcgct ggaagagatc tacgccctgt
    15541 gtagcgccta ttccacaacc tacaacatgc cgctggcact gcatctcgac caccacgaat
    15601 cgctggatga tattcgccgt aaagtccacg caggcgtgcg cagcgcgatg atcgacggca
    15661 gtcacttccc gtttgccgag aacgtgaagc tggtgaaatc ggttgtcgac ttctgccact
    15721 cccaggattg cagcgtggaa gcagaactgg gccgcctcgg cggtgttgaa gatgacatga
    15781 gcgttgacgc cgaaagtgca ttcctgaccg atccacaaga agctaaacgc tttgtcgaac
    15841 tgactggcgt cgacagcctg gcggtagcga ttggtacggc gcacggctta tacagcaaaa
    15901 cgccgaagat tgatttccag cggctggcgg aaattcgtga agtggtggat gttcctctgg
    15961 tgctgcatgg tgccagcgat gtcccggatg aatttgtccg tcgcactatt gaacttggcg
    16021 tcacaaaagt gaacgttgcc acagaattaa aaatagcctt cgctggcgcg gttaaagcct
    16081 ggtttgcgga aaatccacag ggtaatgatc ctcgttatta tatgcgcgtc ggtatggatg
    16141 cgatgaaaga agttgtcaga aataaaatta atgtctgtgg ttcagcgaat cgaatttcag
    16201 cataatcatt agttttttat gatttatccc aatgtacttc ccgattaata tcggggagtg
    16261 ccttaatggt aaaggagata actaaacctt aataaataca tcagtaaaat atcgcaacaa
    16321 taatatattt aaaaaaatta tattattcaa ctttatggtg aggatcacac aatgagcagt
    16381 ccaaatattc tcttaacccg tattgataac cgtctggttc acggtcaggt tggcgtgacc
    16441 tggacatcca ccatcggtgc gaatctgctg gtagtcgtgg atgatgttgt cgctaacgat
    16501 gatattcaac agaaattaat gagtattacc gcagaaacct acggctttgg cattcgtttc
    16561 tttactatcg aaaaaaccat taacgtcatc ggcaaagctg caccgcatca aaagatcttc
    16621 ctgatttgcc gtacgccaca aacggtacgt aaattggtag aaggtggtat tgacctgaaa
    16681 gatgtcaacg tcggcaatat gcatttctcg gaagggaaaa agcaaatcag cagtaaagtt
    16741 tatgtcgatg accaggatct cacggactta cgttttatta aacaacgtgg cgtgaatgtt
    16801 ttcattcagg acgtccctgg cgatcaaaaa gaacaaatcc ctgactaaat ctaaaatcgc
    16861 cttaatattg gtttgaggta ataaaaatgc atgaaataac cctacttcag ggattatccc
    16921 tggcggcgtt agttttcgtt ctggggattg atttttggct ggaagcctta tttttattcc
    16981 gcccgataat cgtttgtacc ctaactggcg ctattctcgg tgatattcag actggcttaa
    17041 ttaccggtgg tctgacagag ttggctttcg ccggattaac ccctgcaggt ggtgttcagc
    17101 cgcccaaccc gattatggcg ggtctgatga ccaccgtcat tgcatggtct acgggcgttg
    17161 atgccaaaac agcaattggt cttggcctgc cgtttagttt gttaatgcag tatgtcattc
    17221 tgttcttcta ttctgctttc tcattattta tgaccaaagc cgataaatgc gcgaaagaag
    17281 cggatacggc agcgttttcc cgacttaact ggacaacgat gctcatcgtt gcttcagcgt
    17341 atgcggtgat tgctttcctc tgtacttacc tggcacaagg ggcgatgcag gcgctggtga
    17401 aagcgatgcc cgcctggctg acccacggct ttgaagtggc tggcggtatt ctgcctgccg
    17461 ttggttttgg cttgctgctg cgcgtgatgt tcaaagcgca atatatccct tacctgatcg
    17521 ccggcttcct gtttgtttgc tacatccagg tcagcaacct gttgccggtt gccgtactgg
    17581 gcgcaggctt tgcggtgtat gagtttttca atgcgaaatc ccggcagcaa gcgcaaccgc
    17641 agcccgttgc cagtaaaaat gaagaagagg actacagcaa tgggatctga aatcagtaaa
    17701 aaagatatca cccgtctggg ctttcgttcg tcactgctac aagcaagctt taactacgaa
    17761 aggatgcagg cgggcggctt tacctgggcg atgttgccgc tcctgaaaaa gatttataag
    17821 gacgacaaac caggcctaag cgcggcgatg aaagataacc tagaatttat taatacccac
    17881 ccgaatctgg tcggattcct gatggggtta ttaatttcga tggaagagaa aggggaaaac
    17941 cgcgacacca ttaaaggcct caaagtggcg ctgtttggcc caatcgccgg gattggcgat
    18001 gcgattttct ggtttacctt gttaccaatt atggcgggga tttgctcatc gtttgccagt
    18061 cagggaaacc tgctggggcc gattctgttc tttgccgttt acctgcttat ctttttcttg
    18121 cgcgtcggct ggactcacat cggttactcg gtcggcgtga aggctattga taaagtgcga
    18181 gagaactcgc agatgattgc ccgttcggca accatcctcg ggatcacggt aatcggcggg
    18241 ctgatcgctt cgtatgtgca tattaacgtg gtgacatcgt ttgccatcga cagtacccac
    18301 agcgtcgcgc tgcagcagga tttcttcgat aaagtcttcc cgaacatttt accgatggcc
    18361 tacaccctgc tgatgtatta cttcctgcgg gtgaaaaaag cgcatccggt gctgttaatc
    18421 ggcgtgacgt ttgttctttc tattgtttgt tctgcattcg gcattttgta aatggaacga
    18481 ggcaccgcgt ctggcggtgc ctctttactt aaggaatttc atcctgtgca aacccttcaa
    18541 caagttgaaa actatacggc gttaagtgaa cgtgccagcg aatatttatt ggccgtgatc
    18601 cgtagcaaac cggatgccgt gatttgcctg gcgaccggag ccacgccatt actgacgtat
    18661 cattatctgg tagaaaaaat ccaccagcag caggttgatg tcagccagct caccttcgtg
    18721 aagctcgacg aatgggtgga tctgccatta acgatgcctg gtacctgcga aactttcctg
    18781 caacagcata tcgtgcagcc gctggggcta cgtgaagacc agctcatcag ctttcgctcc
    18841 gaagagatta atgagacaga gtgcgaacgg gtaacgaacc tgattgcgcg caaaggcggt
    18901 ctggatttat gcgttctcgg gttggggaaa aacggtcatc ttgggctgaa cgaaccggga
    18961 gaaagcctgc aaccggcctg ccatatcagt caacttgatg ccagaacaca gcaacatgag
    19021 atgttaaaaa ccgcgggccg tcctgtgact cgtgggatca ccttaggcct gaaggatatt
    19081 ctcaatgccc gcgaagtttt gttactggtg actggcgaag gaaagcagga tgcgacagaa
    19141 cgttttctca cggctaaagt ctctaccgct atcccggctt catttttatg gctgcataac
    19201 tatttcacat gcatgattga tgagatgtgt cgtcgttaac tcgcctgcta caggcgttgc
    19261 aacgctattc ctgctgcgac gcctgtaatc actctccctg ctgctccagc gcatacttat
    19321 acagcgcatt tttcttcacg ccgtgaatct ctgccgccag cgccgccgct tttttcagcg
    19381 gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag tcttcttcct
    19441 gcgctttatg gccttcgaca atcagcacca tttcgccttt gcgacggttt tcatcttctt
    19501 tgacccacgc cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag gttttggtca
    19561 gctcacgcgc cagaaccacg tagcgggatt cgcctaatac cgcaacgata tcttccaggc
    19621 tatctaacag acggtgggta gattcataaa aaatcagcgt gcgcggctcc gcttcaatag
    19681 cttttagcgc atcacggcgg ccttttgatt tggcaggtaa aaagccttca taacaaaaac
    19741 gatcggatgg taaacccgcg gcgcttaacg cagtgatagc agcacacggc cctggtagcg
    19801 gcaccacgcg aatccccgct tcacgacagg tacgcaccag atggtagcca ggatcgttaa
    19861 ttagcggcgt tccggcatcg gaaaccagcg caatgttttg cccctcttgc agtttcgcca
    19921 gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag cgcaaacaac cgggcattaa
    19981 tcccaaaatg ttgcagcaat aaaccggtgt gacgcgtatc ctcggcggca atcagatcaa
    20041 cggcctgtaa tacctctaat gcacgctggg tgatatcagc cagattgccg attggcgtcg
    20101 gtacaatgta aagctggccc tgagaattat ccgccgattg gtgttgtttc attgtgtcgt
    20161 ccgtattgcc gatttaatat tgagcattgc gtaaaaaaaa tatcactgga tacattatgg
    20221 taccctcaac attttctcgt ttgaaagccg cgcgttgtct gcctgttgtt ctggcagccc
    20281 tgattttcgc cggttgtggc acccatactc ccgatcagtc cactgcttat atgcagggca
    20341 cggcgcaggc tgattctgcc ttttacctgc aacagatgca gcaaagttct gatgatacca
    20401 ggatcaactg gcaattactc gccattcgtg cactggtgaa agaaggtaaa accggacagg
    20461 cggtggagtt gtttaaccaa ctaccgcaag aactgaacga ttctcagcgt cgcgagaaaa
    20521 cactgctggc ggcagagatt aaactggcgc agaaagattt tgctggcgcg caaaacttgc
    20581 tggcgaaaat cacacctgcc gatttagaac aaaaccagca agcccgttac tggcaggcaa
    20641 aaatcgatgc cagccagggg cgtccttcca ttgatttact gcgcgcgtta attgctcagg
    20701 aaccgctgct cggtgcgaaa gaaaagcagc agaatattga cgccacatgg caggcgctct
    20761 cctccatgac tcaggagcag gcgaatacgc tggtgatcaa cgccgacgaa aatattctgc
    20821 aaggctggct ggatttgcag cgcgtctggt ttgataaccg taacgatcct gacatgatga
    20881 aagctgggat cgccgactgg cagaaacgtt atccgaacaa cccgggcgcg aaaatgctac
    20941 caacgcagtt ggttaacgta aaagcgttta aacccgcatc gaccaacaaa atcgccctgc
    21001 tgttgccgct gaatggccag gcagcggtat ttggtcgcac tattcagcaa ggctttgaag
    21061 cggcgaaaaa tatcggcact cagccagtgg cggcccaggt agcagccgca cctgccgcag
    21121 acgtagcaga acaacctcag ccgcaaaccg tggatggcgt tgccagcccg gcacaagctt
    21181 cggttagcga gctgaccggt gaacagcctg cggcccagcc ggtacctgta agcgccccgg
    21241 cgacaagcac cgcagcggta agcgcacccg caaatccatc cgcagagctg aaaatctacg
    21301 atacctcatc acaaccactt agccagatct taagccaggt tcagcaggat ggtgcgagta
    21361 ttgtggtcgg tccgttgctg aaaaataacg ttgaagagtt gctgaagagc aacaccccgc
    21421 tgaacgtgct ggcactgaac cagccggaga acatcgaaaa ccgcgtcaat atttgttact
    21481 tcgcgctttc accggaagac gaagcgcgcg atgcagcgcg tcatattcgt gaccagggta
    21541 aacaagcgcc gctggtgctg atcccacgca gttcattggg cgaccgcgta gccaatgcgt
    21601 ttgcgcaaga gtggcagaaa ctgggcggcg gcaccgttct gcaacaaaaa tttggttcca
    21661 ccagcgaatt acgcgcgggt gttaacggcg gttctggtat tgctttaacg ggtagcccga
    21721 ttactcccag agcgacaacc gactccggca tgacgaccaa caatccaacg ctgcaaacca
    21781 cgccaaccga tgaccagttc accaataatg gcggtcgtgt cgatgcggtg tacattgtgg
    21841 caacgccggg tgaaatcgct tttatcaaac cgatgatcgc catgcgtaac ggtagccaga
    21901 gcggtgcaac gctgtacgcc agttcccgca gcgcacaagg caccgcaggc ccggatttcc
    21961 gtctggagat ggaaggcttg cagtatagcg aaatcccgat gctggcaggc ggtaatctgc
    22021 cgttaatgca gcaggcgctc agcgcggtga acaacgatta ttcgctggct cgcatgtatg
    22081 cgatgggcgt cgatgcctgg tcgttggcaa atcatttctc acaaatgcgc caggttcagg
    22141 gttttgaaat caacggtaat accggaagtc tgacggctaa cccggattgc gtgattaaca
    22201 ggaagttatc atggctacag taccaacaag gtcaggtagt ccccgccagt taaccaccaa
    22261 acagaccggc gatgtgtggg aagcacaagc gcgtcgctgg ctggaaggca aaggactgcg
    22321 gtttatcgcc gctaacgtga acgagcgtgg cggcgagatc gatctgataa tgcgtgaagg
    22381 ccggatcacc gtttttgtcg aggtgcgtta ccgccgctct gcgctttatg gcggcgcggc
    22441 agccagtgtg acccgcagca aacaacacaa attattacag actgcccgct tgtggctcgc
    22501 gcgtcataat gggagttttg atactgtgga ttgccggttc gatgtggtag ccttcaccgg
    22561 gaatgaggtt gagtggatta aggatgcctt taatgaccac tcataattaa ggtttaagga
    22621 ttagcgtgca agaaagaatt aaagcttgct tcactgaaag cattcaaact caaattgcgg
    22681 cggcagaggc gcttccggat gccatctcgc gtgcagccat gacgctggtt cagtctctgc
    22741 tcaatggcaa caaaatcctc tgttgtggta atggaacttc cgctgccaat gcacagcatt
    22801 ttgctgccag catgatcaac cgtttcgaaa cggagcggcc cagcttacct gccattgcac
    22861 taaatactga taatgttgtc ttaacggcga ttgccaacga tcgcttacat gatgaagtgt
    22921 atgcaaaaca ggtgcgggcg ctgggtcatg cgggagatgt attgttagcc atttccaccc
    22981 gtggcaacag ccgcgatatt gtaaaagcgg tagaagctgc cgttacgcgt gatatgacca
    23041 ttgtggcatt aaccggctat gacggcggtg aacttgcagg tttgttaggg ccacaggatg
    23101 tggagatccg cattccttcg catcgtagtg ctcgcattca ggaaatgcat atgctgacgg
    23161 taaattgcct gtgcgatctg atcgataaca cgcttttccc tcaccaggat gtttaaggag
    23221 aatacatgaa ggcattatcg ccaatcgcag tccttctttc cgcgctgctg ttgcaaggtt
    23281 gtgttgccgc tgccgtagtg ggtaccgctg ctgtaggtac caaagccgca actgacccac
    23341 gcagtgtcgg cacccaggtg gacgatggta ccctggaagt gcgcgtgaac agcgcattgt
    23401 cgaaagacga acaaattaag aaagaagcgc gcattaatgt cacggcctat cagggcaaag
    23461 tgctgctggt tgggcagtca ccaaacgctg aactttcggc tcgcgccaaa cagattgcta
    23521 tgggcgtaga cggtgccaac gaagtgtata acgagattcg tcagggccag ccgattggtc
    23581 tgggcgaagc gtctaacgat acgtggatca ccaccaaagt acgttcgcag ctgttaacca
    23641 gcgacctggt gaaatcgtcc aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga
    23701 tggggctggt gactgaacgt gaagcgaaag cggcggcaga tattgccagc cgggtaagcg
    23761 gcgtgaagcg ggtaactacg gcgtttacgt ttattaaata gcagcccttg taatgcctga
    23821 tgcgacgctt gcagcgtctt atcaggccta caacgatgca aaccgtaggt cggataaggc
    23881 gtttacgccg catccgacat gttttccctc aaatcaaacc aacagcgcca gcccgccgac
    23941 aatcacacca gacactgcca ccatcgcccc cgtcagccat aaggctttcg ccgggaacgc
    24001 tttgcgcagc ataatcagtg acggcaaact caccgccggg agcgtcatca acaatgccag
    24061 cgccggagca gttcccatac ctgccagcat catcgtttgt acaatcggaa tttctgctgc
    24121 cgtgggaatg acaaacaagc atcccgctac cgccatcgcc accacccaca tcaggctgtt
    24181 atcgacagca ccatcggcat gggggaataa ccagacgcga gcggcaccca acaccagtac
    24241 tgcaaggatg taaaccggga tcgtactcca gaaaagcgtc catagcgccc tgccccagcg
    24301 gctaaaaaat cctccctgtg cttccggtat gtcaatttcg accggtgcct gcgtttgcgg
    24361 tgtttcacgc acccattttt gcaccagcgt cgcaatcagc aacaccatca ccagccccgc
    24421 caccagacga atcgccgcaa aaccccagcc gaggacgaac cccataaaca ccagcgtcgc
    24481 cgggtttaac aacggattcc ccatccagaa tgccagcgca ccgcccatcg acacctgttg
    24541 gcgacgcatt cccgccgcga ccggagccgc acagcaggta cacatcatgc ccggcagcga
    24601 aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac gcaataacca
    24661 gtcacgcggg atcagcacct gaatcaacga cccgagcagt acgcccagca ccgccgcttt
    24721 ccataccgcg aggaaataga tcatcgcgta atccaacgcc gcctgccacg gattagcatc
    24781 cgcctgcgca aggatagatt taccgatact gtgggtttcg gcagcagtaa acgctttgcc
    24841 gtagtaaggt tgccatttca cataccagag accagcaatg acaacgagaa agaaaagcgc
    24901 gggtttccac cactgaatgg gcgttgccgc ctgagatgaa gactgaccag tcatagcatt
    24961 ccccaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat caattcttgg
    25021 gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca caccttcatg
    25081 ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat caatggattt
    25141 ccagttaccc ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca tccgctgttt
    25201 gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc agttctgggc
    25261 gattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg agtgggcatt
    25321 agcacccata gcactgacca ccagcatatg ctgcgcaccg agccgccgcc cggttaatgc
    25381 ggtatccacc accagcgtgt aatcggcatg aataaacgcc tctttactcc ccgcttctcg
    25441 ccgcgtggtg ccgagacaac aaaacacaat gtcgatggga tcggtgacct gcgccagcgc
    25501 gtcggtcagt tgcggatcat ggggattaaa cacaccgggc atatcgccca acggacgtcg
    25561 cgtcggcgcg gctatggcgt taactttcgg ttcattaatc aacatccgca gcaggtgacc
    25621 acccaccagc cccgttgcgc ctgtaatcag tacctggctc atcttcgctc ctttacagaa
    25681 ttgtctgcct tgcgctcccc ggctcatgca ccaggcttaa tagtctacga ggtaattatc
    25741 cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca tgagtaagaa
    25801 aattgccgtt ttaatcactg atgaatttga ggattcagaa tttacttcac ccgcagacga
    25861 gttccgtaaa gccggacacg aagtgattac cattgaaaaa caagcgggta aaacggtgaa
    25921 aggcaaaaaa ggagaagcca gcgtggccat cgataaatcc atcgatgaag tgacgccagc
    25981 ggagtttgat gccctgctgc taccgggcgg ccattcaccg gattatctgc gtggcgacaa
    26041 ccgttttgtc actttcaccc gtgattttgt gaatagcggc aaacctgttt tcgccatctg
    26101 tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac tgactgccgt
    26161 taaaccgatc attattgatg ttaaaaacgc gggcgcggaa ttttacgatc aggaagtggt
    26221 ggtcgataaa gatcagctgg ttaccagccg tacaccggac gatctgccag cgtttaaccg
    26281 cgaggcatta cgcctgctcg gtgcctgagt tacgtagcca gatcattttt ttaccaaagc
    26341 ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc ggggccgaca
    26401 gcattctggc aaccggaaag cggcgattgt acgcctggcg cgcaaggtcg ctttcctcac
    26461 cttcaagcct gcggatctcg cctttaaatt gcacgccgcg aattaacgct accgttttcg
    26521 gctgaccgtt taccgttccg gcaactgccg cctgcggccc actcatctgc gcgtggcgcg
    26581 ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaaaaga taaaaggcat
    26641 tagcgcacca gagttcccct tcctgctgca cacaccaggt gacaacatgt tgttttgcca
    26701 gccaacggct gatggcggtg agtgtttcca ttgctgttct cttttatact gtgggcctga
    26761 ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg taccgccgac
    26821 gataagcttt ataccgggat caccacagat gtcgaacgcc gctatcagca gcaccaaagc
    26881 ggcaaagggg cgaaagcact gcgcgggaaa ggagaactaa cgctggcgtt ttccgcgcca
    26941 gtgggcgatc gttcgctggc actacgggcg gaatatcgcg ttaagcaact gaccaaacgg
    27001 caaaaagagc gtctggtagc ggaaggcgca gggtttgcag aactgttgag cagcctgcaa
    27061 accccaaaga ttaaaagcga ttgaagtgct cgtgatactc aaccagacca gttacaccgt
    27121 tcagcgcgtc atccgctaaa cgatgtacct ggaaggcact ttcagtgccc ggccagcggc
    27181 aacgcagatc gtgatgtgcc gccagttcaa agccgaaacg gctgtacagc gccggatcgc
    27241 ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct tcatagacca
    27301 gttggcgtgc cagcccctgt ccacggtatt tttcatctac cgccagcggt gccatgccga
    27361 cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag ccaatgacct
    27421 gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca cgcagatcgt
    27481 gaaccagctt cgcttccgca tcactttcga atgaacgacg cagcagggca tcaatacccg
    27541 gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg tctgtttggt
    27601 ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca gcatcatgcg
    27661 taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca catacagccc
    27721 cagctccgta tcgccttcaa ttaccagccg acgctggaag aagagcgtat ccggatcttg
    27781 tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca ctaaaactca catcagcttg
    27841 cgcgttctgg ctaacgacca gtttgccatt caccaccgag gtaaaccatt gcaggtcaat
    27901 atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc catcatccaa
    27961 cgcctggcgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta gcgcaaatgg
    28021 cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac gggaacgcag
    28081 tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc catatcagaa
    28141 aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc accaatacaa
    28201 ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc cagttcgtta
    28261 acatttttgc gctttgatag cgcaaccttc aggaaaaatt atggagctgc tctgccctgc
    28321 cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg tttatatcgg
    28381 gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg agaaaaaatt
    28441 gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg cgattaacac
    28501 ttttgcgcat ccggacggtt acgcccgctg gcagcgcgcc gtggatatgg cggcgcagtt
    28561 gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gagtacgccg ccgagcgtta
    28621 cccgcatatt gagcgtcatg tgtcagtgca ggcttcggcg accaatgaag aggcgattaa
    28681 cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt tgtcgattca
    28741 tcaggtgaaa caactggcac gggtcacacc tgtcccgctg gaagtgtttg ctttcggcag
    28801 cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg gtgagtcgcc
    28861 caacaccgtg ggcgcgtgtt ctccggcccg tttcgtgcgc tggcagcaaa cgccgcaggg
    28921 actggaatct cgcctgaacg aagtgctgat cgaccgttat caggatggcg aaaacgcagg
    28981 ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc acgcgctgga
    29041 agaacccacc agtctcaata ccctggaact gctgccggag ttaatggcgg cgaatattgc
    29101 ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg tggcgaaagt
    29161 ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa aacttcgtac cgcaaagcgc
    29221 gtggatggag acgctcgggt cgatgtccga aggcacgcaa actacccttg gcgcgtatca
    29281 ccgtaaatgg cagtgagaaa agcaatgaaa tattccttag ggccggtgct atggtactgg
    29341 ccaaaagaga cgctggaaga cttttatcag caggccgccg ccagcagcgc agacgtgatt
    29401 tatcttggtg aagcggtctg tagcaaacgc cgggcaacca aagttggcga ctggctggag
    29461 atggcaaaat cgctcgccgg aagtggcaag cagattgtgc tctccacgct ggcgctggtg
    29521 caggcgtcct ccgaacttgg cgaactgaaa cgttatgttg agaacggtga gtttctgatt
    29581 gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc gttcgtcgcc
    29641 gggcacgcgc tgaactgcta caacgcggtg acactgaaaa tattgctcaa acagggcatg
    29701 atgcgctggt gtatgccggt ggagctttcc cgcgactggc tggtgaatct gcttaatcag
    29761 tgcgatgagc tgggcattcg caaccagttt gaagtggaag tcctgagcta cggtcatctg
    29821 ccgctggcct actccgcccg ctgctttacc gcgcgttcgg aagaccgccc gaaagatgag
    29881 tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg tgctatcgca ggaaaaccaa
    29941 caagtgtttg tactcaatgg cattcagacc atgagcggct acgtttacaa cctcggtaac
    30001 gagctggcat ccatgcaggg gctggtcgat gtggtccgcc tgtcaccgca gggtactgac
    30061 actttcgcga tactcgacgc cttccgcgcc aatgaaaatg gcgctgcacc actgccgttg
    30121 acggcaaaca gcgattgtaa cggctactgg cggcggctgg ccgggctgga gctgcaagcc
    30181 taagtaaata gctcactttg ttaacaactt taactactct ttaatgcagt attaaagatt
    30241 aatcagtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg ctactcgatc
    30301 tggcccccat tcccgaaggc tcttcagcgc gggaagcgtt ctcccactct ctcgacctcg
    30361 cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcggaacac cacaatatga
    30421 ccggcattgc cagtgctgca acatcagttt tgattggcta tctggcggcg aataccacta
    30481 cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccattg gtgatcgccg
    30541 aacagttcgg cacgcttaat acgctctatc cggggcgaat cgatttgggg ctgggtcgtg
    30601 cgccgggtag tgaccagcga accatgatgg cactacgtcg tcatatgagc ggtgatattg
    30661 ataatttccc ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc gatcccaatc
    30721 cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca
    30781 gcctttacag cgcgcaactg gcggcgcagc ttggtctgcc gtttgcgttt gcctcacact
    30841 tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagcaacttc aaaccgtcgg
    30901 cacgactgga aaaaccatac gcgatggtgt gcatcaatat tatcgctgcc gacagcaacc
    30961 gcgacgctga atttctgttt acctcaatgc agcaagcctt tgtgaaacta cgccgtggcg
    31021 aaaccggaca actaccgcca ccgattcaaa atatggatca gttctggtca ccgtctgagc
    31081 agtatggcgt gcagcaggcg ctgagtatgt cgttggtagg tgataaagcg aaagtgcgtc
    31141 atggcttgca gtcgatcctg cgcgaaaccg acgccgatga gattatggtt aacgggcaga
    31201 ttttcgacca ccaggcacgc ctgcattcgt ttgagctggc gatggatgtg aaggaagagt
    31261 tgttggggta gtgtgtttta acgtaggaag ccttatccga gatggcaacg ctgtcctacg
    31321 taggcctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc
    31381 accgctgatt actgatacac cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg
    31441 ttgccaacgc caaataccag aatcagcata atcatcggct tgccgcccca gacgcggaat
    31501 ttcgggctac caaagcgttt acgcgatgcg cgggccaaca gcgccggaac aattgccgcc
    31561 cagatggtag ccgctaagcc agcataacca atggcgtaca ggaatccgtt aggaaacagc
    31621 aggccaccca caacaggcgg ggcaaaggtc agcaacgctg ttttcaaacg gcccatagcc
    31681 gagtcgtcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg
    31741 aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gaagatccag gctacggctg
    31801 ttcagcacgc cgcttaatgc ctgtaccagc acatcaatat taccgccctt ctcggcgatg
    31861 ccgataaact ccggacgagg gatgttgccc atcgtcgcca gcaaccagat ggtatacagc
    31921 gccagcgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg
    31981 taatacttca tcaggctcgg cacgttaccg tgataaccaa acgatgccag acagaatggc
    32041 agtgtcatca gcagatacgg cgcataagac gcattgcttt cggcgacgtt gaacaaggtc
    32101 gttggctgta catgccccag caggctgccg aaggtgagga agaaagtaat gactttcgcc
    32161 cccagcacaa ttgctgtcat ccggctgaca gctttcgtac tcaaccacac cacgaacgct
    32221 accagcagtg caaaaccaaa tccagctgcc cgtgcaggga cgttcagcga catctccgcg
    32281 aaggtgtgat gcagaatcga accgctggca gaaatatagg cgtaagtcag gatatagagc
    32341 acaaaggcaa tggaaatgcc gttgactacg ttccagcctt tgcccagcaa atctttggtg
    32401 atggtgtcaa agctagaacc gattcgataa ttcaggttag cttccagaat catcaagccg
    32461 gaatgcagca tacagaacca ggtaaagatc agcgccgcca ttgaccagaa gaaccacgcc
    32521 ccggacatga ccacaggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc
    32581 accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct
    32641 tctctccagt gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac cagtacacga
    32701 gtacaaaaga cagaaaaaaa gccccgatgg taaaaatcgg ggctgtatat attattttac
    32761 agattgtgtt cgctgttcag cgatgattac gcatcaccac cgaaacgacg acgaccggta
    32821 gagtcatcac gacgcggagc gcggccttca cgacgttcgc cgctaaaacg acgaccatca
    32881 ccacgaccac cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgttcgcca
    32941 ctgaaaccac gaccaccgcc acgacgctca ccgccagtat gcggctgtgc atcgcccagt
    33001 aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg cagcacttca
    33061 cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt gatgttacca
    33121 atgtaacggc tgctgatgtc gccttcgtta gcaatcgcac caacgatatg acgaacttca
    33181 acaccatcat cgcggcccac ttcaatgcgg tacagctgca tatcgccaac atcacgacgt
    33241 tcacgacgcg gacgatcttc acggtcacca cgcgggccac ggtcgttacg atcgcgcgga
    33301 ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggaaca
    33361 atcagagtac gttcaccctg tgccattttc agcagtgcgg cggccagagt ttcgagatcc
    33421 agctcttcac cttctgccgt cggctgaatt ttgctcagca gtgcacggta ttgatccaga
    33481 tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg acgtttgccc
    33541 agcagttctg cgttcggcag ttcaacttcc ggaatggtca gcttcatggt gcgttcaatg
    33601 ttacgcagca gacgacgctc acggttctca acgaacagca gcgcacggcc agcacggccc
    33661 gcacgaccag tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag
    33721 ttaactacca ggctgatacg ctcaacgtcc aggccacggg ccgcaacgtc ggtcgcaatc
    33781 aggatgtcca gacgaccatc tttcagtcgt tccagtgtct gctcacgcag cgcctggttc
    33841 atgtcaccgt tcagcgcggc gctgttgtag ccgttacgct caagggcttc tgccacttcc
    33901 agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg
    33961 aaacgtacca gtgcttcgtt tttacgcata ccccagacag tccagtagct ctggctgatg
    34021 tcaggacggg tagtcacgct ggactgaatg cgcacttcct gcggctcttt cataaagcgg
    34081 cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct
    34141 tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg
    34201 tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg
    34261 tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt
    34321 aattgcacgt cataacgttg gccgccgtac agggcaacca cgtttacgcc gcgcatgtgt
    34381 ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc
    34441 accagaatct gtggtgcttt cagctcagga tcaagattct gcagcagagg taaagagaat
    34501 gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga
    34561 tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct
    34621 tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag
    34681 tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc
    34741 aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc
    34801 tcacccgtta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt
    34861 gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg
    34921 cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg
    34981 tggcgctgtc caaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac
    35041 tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt tccattaacg
    35101 tttgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat
    35161 ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg agcttctgct
    35221 cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca
    35281 gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc ccgcgattca
    35341 agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag
    35401 catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaatact tcaggcatat
    35461 ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac
    35521 tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc
    35581 gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca
    35641 atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa
    35701 gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac
    35761 ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc
    35821 ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg
    35881 gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct
    35941 ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg taatcggtca
    36001 ctttctcaac gcgtttgtca gcgatctgag agatgtgtac cagaccttct ttaccgccgc
    36061 cgatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccattg tagacgcggc
    36121 ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcatgtttc gctttctcgc
    36181 cgtcggtcgc tgcgattttc acagtaccgt catcttcgat ttcgatggtg gtgccagttt
    36241 cttcggtcag agcacggata acagaaccgc ctttaccgat aacgtctttg atcttgtccg
    36301 ggttgatctt gatggtatgg atacgcggag cgaactcaga gatatcgccg cgcggcgcgt
    36361 tgatcgcctg ttccattacg cccaggatat gcagacgcgc acctttagcc tggttcagcg
    36421 caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc aatgcagaga
    36481 taccgtcacg ggaacccgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca
    36541 aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg
    36601 caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg
    36661 aagccataga agaggaaccg ttggattcgg tgatttcaga cacaacacgt acggtatacg
    36721 ggaatttgtc catatccggc atgactgcca gcacgccgcg cttcgccaga cgaccgtgac
    36781 caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacagag tacggaggga
    36841 agttgtagtg gaacaggaag gtgtcagtac gttcgcccat cagttcatca agaacctgcg
    36901 cgtcacgtgc agtacctagc gttgcggtaa ccagcgcctg agtttcacca cgggtgaaca
    36961 gcgcagaacc gtgagtacgc ggcagcacac cagtacgcac atccagacca cggatcatat
    37021 ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacatttt
    37081 tctcgatagc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa
    37141 gcagtgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt
    37201 cggtgatgcg gtaagcatcg ctcagacgag cttcagccag tgcagcaacg cgcgcgttca
    37261 gcgcttcgtt taccggctcc ggctgccagt cccaacgcgg tttaccggct tctttcacca
    37321 gttcattgat gttctgaata acaacctgct gttgttcatg accgaagact actgcgccca
    37381 gcatctggtc ttcgctcagc agttcagctt cagattcaac catcagtaca gcggcttcag
    37441 tacccgcaac aaccagatcc agtttgctct ctttcaactc gtcctgagtc gggttcagta
    37501 cgtactggtc attgatgtaa cctacacggg cagcaccaat cgggccattg aacggaatac
    37561 cagacagaga cagggctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg
    37621 ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca
    37681 gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttgggcggc
    37741 cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt
    37801 taacggtcag cgggaagaag tcctgacctg gtttggcttt tttctgacca acaacggtaa
    37861 cgaataccgc agtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca
    37921 tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa
    37981 gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg ttaatacccg
    38041 atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc
    38101 tctcattagc cgcgcgaacc tctgcagcgg aagatcattc atagcaacaa tacattagtt
    38161 tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggccccc ttttctgaaa
    38221 ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt
    38281 ctttacgttt caggtagtcg agcagtttac gacgctgaga aaccatgcgc agcagaccac
    38341 gacggctgtg gtgatctttt ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg
    38401 cagtcagcag tgctacctga acttcggtag aaccggtgtc gtttgcgtca cgaccaaact
    38461 cagaaacgat tttagctgtt gcttcagtac ttagagacat tttaaaactc caaagtatat
    38521 agaatgaaag gacgccgatc tctaattcag cgatcccagt gtacgttacg caaagtgtta
    38581 aacaatttac gcgacgttaa gcggcagtat tctactcgta gcgacttgtt atcgcaagac
    38641 ggttaacatt acgccgggta ttcaaccacc aggcgacgag gcgcaacgcg gccttcatcg
    38701 tcaatttcgc ccataccgat aaacttgccg ttctcaccct ctgtaacgcg aaccagtcct
    38761 tccagcggcg caccggatgt acgaactggg ttaccatttt tgaagtaaac agaagacgtt
    38821 aacggaagat tcaccactgg atagtccgaa gctggactgt ccattggcat cagtaatgga
    38881 tcaagtaact ccgcagccgg aatatcctgc tgttcggctt gctcaaccag ttcacgcaga
    38941 tgttccaggg tcaccatccg ttcaaccgga tatttactta ccgccagacg acgcaagtaa
    39001 ataacatgcg cgccacaacc gagtttttcg ccgagatcgt caatgatagt gcggatataa
    39061 gtgccttttg agcagtgaat ttccagctcc agctcattgc cttcatggcg aataaacagc
    39121 aattcataaa cggtaatcgg gcgcgcttca cgcggaactt caatgccctg acgcgcgtat
    39181 tcgtacagtt ttttgccctg atatttcagt gctgaataca tcgaagggat ctgttcgata
    39241 tcgccacgga aggtatccag cgccgccgcc agttgctctg cactaaaggt taccggacgt
    39301 tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg
    39361 acccgataac gtttgtcgga gtccagcaga tactgggaaa acttcgtcgc ttccccgagg
    39421 caaattggca acatgccggt cgccagcggg tccagcgcac cggtatgccc ggcacggttg
    39481 gcgttataaa tccgtttcac tttttgcagc gcatcgttgc tggacatccc ctgcggttta
    39541 tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac gaggacgact cattagtcct
    39601 ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg tttgaccacg ctggtcacca
    39661 ggtttgacat gcgcatccct tcaaccagag agttgtcgta gaagaaggtc agttccggca
    39721 cgatacgcag gcgcatcgct ttccccagca ggctgcggat gaaaccagaa gcttcctgca
    39781 gcgctttgat gcccgcttta accgcgtctt catctttgtc gttgaggaac gttacatata
    39841 ctttggcata cgccaggtcg cgagacattt cgacaccgga aacggtggtc atcatgccca
    39901 gacgaggatc tttaatttca cgctgcagga tgagagcgat ctctttttgc atttcctgcg
    39961 ctacgcgctg cgggcgacca aattctttcg ccataataaa ttctcctgac aaaaaagggg
    40021 ctgttagccc ctttttaaaa ttaatttcag gtggaagggc tgttcacgtt ggcctgataa
    40081 gacgcgccag cgtcgcatca ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc
    40141 atggaaccct aaaaacctta agcgatggta cgttggattt cgatgatttc gaatacttcg
    40201 atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc cgataccaca ttccataccg
    40261 ttacggactt cgttaacgtc atctttgaag cggcgcaggg actccagctc gccttcgtag
    40321 ataaccacgt tgtcgcgcag aacgcggatc gggttgtgac gtttaaccac accttcggta
    40381 accatacaac ctgcgatggc accaaatttc ggtgatttga atacgtcacg aacttccgcc
    40441 agaccgataa tctgttgttt cagttccgga gacagcatac cgctcattgc cgctttcact
    40501 tcgtcgatca ggttatagat gacggagtag taacgcagat ccaggctttc cgcttcaatc
    40561 actttacgtg cagaggcatc agcacgtacg ttgaagccaa ccaggatggc attggacgcc
    40621 gcagccaggg tagcgtcggt ttcggtgata ccacctacgc cagaaccgat gatcttcact
    40681 ttaacttcgt cagtagacag tttcagcaag gagtcggaga tagcttccac agaaccctgt
    40741 acgtctgcct tcaggacgat attcacttcg tgaacttcgc cttcggtcat gttggcgaac
    40801 atgttctcga gtttagattt ctgctgacgc gccagtttaa cttcgcggaa tttaccctga
    40861 cgatagagtg caacttcacg cgctttcttc tcgtcacgta caacggtaac ttcatcaccc
    40921 gcagccggaa cgccggacag gccgaggatt tccaccggaa tggacggacc cgcttccagc
    40981 acttcctgac ccagttcgtt gcgcatcgca cgaacacgac cgtattcgaa gccacacaga
    41041 acgatatcgc ccttgtgcag agtaccttca cgtaccagaa cggtagcaac cggaccacga
    41101 cctttatcga ggaaggattc gataaccgca ccgctcgcca tacctttacg taccgctttc
    41161 agttccagaa cttccgcctg cagcaggata gcgtccagca gctcatcgat accagtaccc
    41221 gctttcgcag acacatgtac gaactggctt tcaccgcccc actcttccgg caggatgccg
    41281 tactgggaga gttcgttctt aacgcgatcc ggatcggctt ctggtttatc gatcttgttc
    41341 actgcaacca ccaccggaac ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc
    41401 ggcattacgc cgtcgtcggc agcaacaacc aggactacga tgtccgttgc ctgcgcacca
    41461 cgagcacgca ttgaagtaaa cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg
    41521 ttttcagttt caacgtggta tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag
    41581 gccactttcg ttgaacgaat gtagtccagc agagaggttt taccgtggtc aacgtgaccc
    41641 atgatggtca caaccggcgc gcgcggttca gccgctgcac ccgtgtcacg gtcgctcatt
    41701 accgcttctt ccaactcgtt ttcacgacgc aggataactt tgtggcccat ctcttcagca
    41761 accagctgtg cggtttcctg atcgataacc tggttgatgg ttgccattgc gcccagtttc
    41821 atcatcgctt tgatgacctg agagccttta accgccatct tgttcgccag ttcgccaacg
    41881 gtgatggttt cgccgatcac aacgtcacgg ttaacggcct gagcaggctt ctggaagcct
    41941 tgctgcagcg aagaaccttt acgttttccg cccttaccgc cacgtactgc tgcgcgtgct
    42001 tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt tcttcggacg cgcagctttc
    42061 gcgttacgac cacggccacg gccgccttcg acttcacgat cgctttcgtc ttctgcctgg
    42121 cgagcatgtt gagaagtagt aacgtgataa tcgctggaat cttcagtcgg ttccgcgtta
    42181 tcagtccatt tgttttcttc cgccatacga cgtgcttctt cagcaacgcg acgtgcttct
    42241 tcttcgagtt tacgacgcgc ttcttcttcg gctttacgct tgagctctgc agcttcctgc
    42301 tcacggcggg ctttttcagc ctgggcgttt ttagtcatat cgtcttgttg attgctcact
    42361 ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag cttcacgctt agcttgttct
    42421 gcggcctcac gttcagcttt ttgttgcgcc tcacgtttgg ccgattcttc tgcctcacga
    42481 cgggcttgct cttccgcttc acgctgcgct tgctcttccg cagcaaggcg ttcagcctct
    42541 tgcggatcgc gtttcacaaa ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt
    42601 tttccaccgg taccaggaat gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg
    42661 tccgggcctg aatttttctg attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac
    42721 acagagtcgt cagcagactt ccggatacct gcatcagcaa attgctgtac caggcgttcc
    42781 acggaggtct gtcgctctgc ggccagcgtt ttaatcgtta catctgtcat gctgttcctt
    42841 cctgctacag tttattacgc ttcgtcaccg aaccagcaaa tattacgggc agccataatc
    42901 agtgctccgg ctttttcgtc ggtcaaccct tcgatatcag ccagatcatc aatgccctgt
    42961 tcggcgagat cttccagcgt acaaacgcca cgggcggcca gtttgaatgc caaatcacga
    43021 tctatccctt caaggttcag cagatcgtca gccggtttgt tatcaccgag gctttcttcc
    43081 tgggcctgtg caatggtggc cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc
    43141 ggctcatcaa ggccttcgat ttccaacagc tctttcatcg gcacataggc cagttcttcc
    43201 agcgtcgaga agccttcttc taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat
    43261 ttggtgaagg tgtcgatcgc tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca
    43321 acggtcatca cgttgagttc ccaaccgctc agctgcgaag ccagacgcac gttctgacca
    43381 ttacggccaa tcgcctgcgc caggttaccg gcttcaacgg cgatatccat ggtgtgttta
    43441 tcttcatcca ccacgataga agcaacgtct gccggtgcca ttgcgttaat cacgaactgc
    43501 gccgggttat catcccacag gacgatatcg atacgctcgc cgcccagttc agtagaaacc
    43561 gcctgaacac gcgcgccacg catacctacg caagcaccta ccggatcgat acgtttatcg
    43621 ttggttttca ccgcgatttt cgcacgagaa cccggatcgc gagccgctgc tttaatttca
    43681 atcacttctt cgccgatttc cggcacttca atgcggaaca gttcgatcag catttccggc
    43741 ttggaacggg tgacgaacag ttgcgcgcca cgcgcttccg ggcgaacgga atagagcacg
    43801 ccacgaacgc ggtcgccagg acggaagttt tcacgcggca gcatatcttc gcgcaggatc
    43861 acggcttcag cgttgttgcc cagatccaga gagatgttgt cgcggtttac ttttttcacc
    43921 acgccggtga tgatttcacc ttcgtgttca cggaactgat caaccaccat cgcacgttcg
    43981 gcttcacgca ctttctgcac gataacctgt tttgccgtct gggtggtgat acggtcaaag
    44041 gtaacagact caatctgatc ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa
    44101 cgtgcggctt caagggtgat ttccttggtc ggctgggtga cttcatcaac aactaaccag
    44161 cgacggaagg tgtcaaaatc accgcttttg cgatcgatct gtacacggac gtcgatctct
    44221 tgctcatatt ttttctttgt tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc
    44281 tcgcgaggta gcgccttttc attggatacg gcttcaacta cagccaaaat ttctttgttc
    44341 atcgcgggct tttcacctca tccagactat taaaagtggg gaaccaggtt cgccttctgg
    44401 atattactca gcgcgaacac ttcatcttta ccttcgacgg taactgtgat catttcaccg
    44461 tctaccgctt tgataacgcc ctgccattta cgacggtttt gtaccgccat gcggagaacc
    44521 agagtcacct cttctccgac gaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg
    44581 agacccggtg aggagacttc caggttataa gcaacggtga tgggatcttc aacatccagc
    44641 acagcactta cctggtggct cacatcagca caatcatcaa cattgatgcc atcttcacta
    44701 tcaatataga tgcgcagtgt ggatgtgcga ccgcgaataa attcgatgcc aaccagttca
    44761 aaacccaggg cctcaactgg cgcagtaatc atctctgtta atttttgctc taatgtggac
    44821 aagcccaccc ccaagacata aaaaaagggc ctaaagccca gttattctgt agtcagataa
    44881 caaaaaaccc cgataaatcg gggctttata taactgaacc ctataaccgc aactgcggtc
    44941 tggagcactt tccagaagga ttttttcaaa tcccactacg aaggccgaag tcttcacagt
    45001 atatttgaaa aaggactcta agggaaagtg gttgcggggg ccggatttga accgacgacc
    45061 ttcgggttat gagcccgacg agctaccagg ctgctccacc ccgcgcctga aacgtggcaa
    45121 attctactcg ttttgggtaa aaaatgcaaa tactgctggg atttggtgta ccgagacggg
    45181 acgtaaaatc tgcaggcatt atagtgatcc acgccacatt ttgtcaacgt ttattgctaa
    45241 tcacgtgaat gaatatccag ttcactttca tttgttgaat acttttgcct tctcctgctc
    45301 tcccttaagc gcattatttt acaaaaaaca cactaaactc ttcctgtctc cgataaaaga
    45361 tgattaaatg aaaactcatt tattttgcat aaaaattcag tgagagcgga aatccaggct
    45421 cctcatcagt taattaagca gggtgttatt ttatgacgac gattctcaag catctcccgg
    45481 taggtcaacg tattggtatc gctttttccg gcggtctgga caccagtgcc gcactgctgt
    45541 ggatgcgaca aaagggagct gttccttatg catatactgc aaatctgggc cagccagacg
    45601 aagaggatta tgatgcgatc cctcgtcgtg ccatggaata cggcgcggag aacgcacgtc
    45661 tgatcgactg ccgcaaacaa ctggtggccg aaggtattgc cgctattcag tgtggcgcat
    45721 ttcataacac cactggcggc ctgacctatt tcaacacgac gccgctgggc cgcgccgtga
    45781 ctggcaccat gctggttgct gctatgaaag aagatggcgt gaatatctgg ggtgacggta
    45841 gcacctataa aggaaacgat atcgaacgtt tctatcgtta cggtctgctg accaatgctg
    45901 aactgcagat ttacaaaccg tggcttgata ctgactttat tgatgaactg ggcggtcgtc
    45961 atgaaatgtc tgaatttatg attgcctgcg gtttcgacta caaaatgtct gtcgaaaaag
    46021 cctactccac ggactccaac atgctcggcg caacgcatga agcgaaggat ctggaatacc
    46081 tcaactccag cgtcaaaatc gtcaacccga ttatgggcgt gaagttctgg gatgagagcg
    46141 tgaagatccc ggcagaagaa gtcacagtac gctttgagca aggtcatccg gtggcgctga
    46201 acggtaaaac ctttagcgac gacgtagaaa tgatgctgga agctaaccgc atcggcggtc
    46261 gtcacggcct gggcatgagc gaccagattg aaaaccgtat catcgaagca aaaagccgcg
    46321 gcatttatga agcccctggg atggcactgc tgcacattgc gtatgaacgt ttgttgaccg
    46381 gtattcacaa cgaagacacc attgagcagt atcacgcgca tggtcgtcag ttgggccgtc
    46441 tgctgtacca gggacgttgg tttgactccc aggcgctgat gctgcgggac tctctgcaac
    46501 gctgggttgc cagccagatc actggtgaag ttactctgga gctgcgccgc gggaacgatt
    46561 attcaatcct gaataccgtc tcagagaacc tgacctacaa gccagagcgt ctgacgatgg
    46621 aaaaaggcga ctcggtgttc tcgccagatg atcgtatcgg tcaattgacc atgcgtaacc
    46681 tggatatcac cgatacccgc gagaaacttt tcggttatgc caaaactggc ctcctctcct
    46741 cctctgccgc ttcaggcgtg ccgcaggtgg agaatctgga aaataaaggc cagtaattcg
    46801 acatcaaccc tgcccttcag gggtggggtt accttgctta tcctctgtaa actcctttct
    46861 gagcatcaca taatcgaaaa catcatggtt tgcatccaca acccgtgagt ctcctttata
    46921 gtgtgcaatc acttcctcca gcgttttcgg ttgttccggc tttgttaccc ccatccacgc
    46981 attaatcagg taattattgt aagctgtgga aaagatgttg ttttccgttg tgcgatccac
    47041 gccatcccca agaacggggc tataccagat aaacatcggg acatgatatg cctgctggct
    47101 ggcctccctg cctccatgaa aatagacgtt cttcttcgta gggtcacgtt ccaggccgtg
    47161 gtcggcaaaa tacatgaccg aggcgcggcg atcttttaat aattcaaaaa cctgacctag
    47221 caaactatct gtgtaatgaa tggagttgtc atagcaggca tcctgatcgt cctgcggttg
    47281 aaacacggcg ctggattgcg gataggcgct acaagccggt tcatggcttc catttaaatg
    47341 aagaacaatc agtttctttt gctgcgtatt ttgctgtaat gcttgcgata aatgcggcaa
    47401 caacaattca tcaaatcctc tgacataaac tgtttccatg gcgcgcatgg cgatactggt
    47461 aactgctgta ccattctgcc gaaaagcgga ttgcgagctt agccagaaag tctgaaatcc
    47521 tgcttgatta gccatattaa taatgttgtc cgggtaatta tgaatgtcat gactcaaaac
    47581 ggaatcagca gttaaagaaa ggggaaccga cagcgcagtg taaggtgcac cgcttattgc
    47641 ttgattaaac agtttgatct gttttctttg tgcttcgact tgcggtgtcg tagagcgtgt
    47701 atatccatac aaagacatat tgtcgacgcg tacagactcc cccacaatca acacgtaggt
    47761 atcaatacct gtatccctga ctgataattg aaaatacggc accgtgtttg caattgagag
    47821 taatctttga tgctctttcg ctgctaaagc aaaataattg agattgaaaa acggtgtata
    47881 ggtagcaaat cgcgacgcta gtatatatgg actgaacgct tttttatttt ttgcatcttt
    47941 ataagcaaat tgacaagcgg aaaataaact gcccgaaatg acaatcagca ataatattcc
    48001 tgtcactttt ttcgtcggca aggaaacatc atattttatt attactgcca aaaaaagaag
    48061 ggataaaaag gcaaaagcac atagataagg gatatacatc cccagcattt tgacaacttc
    48121 atccggatca ctctgcagca cactaatcgc aaatccatca ttgaaagttg taccaaaggt
    48181 acaccatgaa tagaggctaa tactcatgtc tgccgctgtc agtaacacaa atggtgcggc
    48241 aacaagcagg cgaagcggaa gggatgatat acgtttaaca accaggatgg tcagaacgaa
    48301 gaacagcaat cgctgaacca tatgcgctcc ggaagaggcg actaagttcg taataccaaa
    48361 aacaataaca caaagataca acaaccaatg agatttaaaa cttctaacaa atttgttgaa
    48421 tactgtcatc cagaaatcca agttcacaac caatataaac aattatcaac aatgttcaat
    48481 tttatatcag acatgttttt caactctgat ccatatcaga ccgaaacact gttagttttt
    48541 tctttttaaa tgatgcggcc attgttctca gtaattaaat aacattatca atgcgtctct
    48601 aaacattacg cagaacgtag ccatcagtag ttatactgac aggagcagga tggtttatcg
    48661 agaagtaaac atatccgtat tcgtaccgga actggagttg acacaataaa gtgccaatta
    48721 tgtcagcaga agggaaaagt ttataactaa ggcgtaccga taccatcgtt tcaaagtacc
    48781 agctacgagt aaagcaactg gacgagatac agatacctga caaccattcc tcagaccagg
    48841 accaaaacga aaaaagacgc ttttcagcgt ctcttttctg gaatttggta ccgaggacgg
    48901 gacttgaacc cgtaagccct attgggcact accacctcaa ggtagcgtgt ctaccaattc
    48961 caccacctcg gcacggatac tactttttag ttcgggatat cgctggtcgg cttagccgga
    49021 gctgctggct gaatttgttc ggttttcgcc ggcgcactca ggttttccca ttcgctacct
    49081 ttattggttt tgttgctatt gatgttaccc agcaccagac tgatgatgaa gaataacgtt
    49141 gccagcagcg ctgtcatgcg ggtcatgaag ttaccagaac cacttgaacc aaacagcgta
    49201 gcggaagcgc ctgctccgaa ggaggctccc atatcagcgc ctttaccttg ctgcagcatg
    49261 atcagaccaa caaggccaat tgccacaata aggaaaacta ctaaaagagc ttcgtacata
    49321 atcaacctgt tccttgcgga gttgccgcgt accaatgctt caaccaataa agcgggagtt
    49381 tttaatcttt cccactgaag cgggtgtgaa tactaaccaa agcgaatgac cttcgcaagg
    49441 gcaattttat tgcattgtat caactgcgga aaaaaacagc aaaaccgatg tgttcgctga
    49501 gaaaaaaggc gacaatcgcc gcctttttag ccagttattt atcgttttaa acggctttta
    49561 ctgcatcggc gatgcggtgt gcaaattcag tcacctgcgc ttcgtcttcg ccttccacca
    49621 tcacgcgaat taacggttcg gtgccggatt tacgcaacag cacacgtcca cggctaccca
    49681 gcgcagcttc aacttctgcg gtcacagctt tgactgactc atgctccagc ggatcgccgc
    49741 tgcctgctgt gtaacgcacg ttaaccagaa tctgcgggaa cattttcatg ccgctgcaaa
    49801 ggtcgtgcag gctcatatgg ttacgcgcca tcgccgccag cacctgcaag ccagcaacaa
    49861 tgccgtcacc ggtagtggtt ttatccagca ggatcacatg accggagttc tctgcaccga
    49921 tacgccagcc tttctcctgc attttttcca gtacgtagcg gtcacccact ttcgcgcgcg
    49981 caaatggaat acctaactgt ttcagcgcca gttcaagccc catgttgctc atcaatgtac
    50041 ccacagcacc accacgcagc tggccctgac gaagaccttc acgcgcgatg atatacatga
    50101 tctgatcgcc atcgacttta ttgccttcat ggtcaaccat aatcacgcga tcgccgtcgc
    50161 cgtcgaaggc aataccgaga tccgcttttt cagccagcac acgagcctgg agcgcgcgaa
    50221 cgtcggtagc ccccacttcg gcattgatgt ttacaccgtt cggctcacaa ccgatagcga
    50281 taacgttcgc ccccagttcg cgcagcacgt tcggcgcgat gtgataagtc gcaccgtttg
    50341 cacaatccac cacaatcttc agttcactga ggctaagttc gttcgggaac gtggctttgc
    50401 aaaactcgat atagcgaccc gcagcatcaa cgatacggct ggctttaccc agttctgccg
    50461 aatcaacgca gctgatctct ttttccattt ccgcttcgat ggcctcttct accgcatccg
    50521 gtagtttggt gccgtcgata gagaagaatt taatgccgtt atcgtagaat gggttatgcg
    50581 atgcagaaat tacaattcca gcctctgcgc ggaaggtacg cgtcagataa gccacggccg
    50641 gtgttggcat cgggccagtg aagagcgcgg aaagaccggc tgccgcaagc cccgcttcca
    50701 gtgctgactc cagcatatag ccagaaatac gcgtgtcttt accaataata atcttacggg
    50761 agccgtggcg cgccagcact ttacccgcgg cccaacccag cttaagcaca aaatcagggg
    50821 tgatcggcgc atcccctaca cgaccacgaa tcccatcggt accgaaatat ttacgattac
    50881 tcatagcgtt tgttttcctt tgcagacaga gtggcttcca ccacccgcat cgcttctacg
    50941 gtttctttga cgtcatgaac acgaatgatg tgcgcgcctt gcattgcggc aatgaccgca
    51001 caggccagac taccgctcaa acgctcggac ggccccacgt tcagcagctg tccaatcatc
    51061 gattttcgtg acatacccac caacagcggc aggttgaaat ggtgaaattc agccaggcgc
    51121 gccagtaatg aatagttatg ggagagattt ttaccgaaac cgaatccggg gtcgagcaac
    51181 aatttctctt ttgcgatacc cgcctgctcg caacgtgcta tttgctcaat aaagtagcga
    51241 ttcacttctg caaagacatc gtgatacttc ggggcttcct gcatggtttt tggatttccc
    51301 tgcatatgca tcagacaaac cggtaaaccg gtttctgcag ccgcctccag agcgccaggt
    51361 tcggaaaggg agcggatatc gttaataatg tgagcgccaa ctttcgctga ctcacggatg
    51421 acttctggtt tggatgtatc taccgagatc cagacttcga agcgttgagc aattgcctca
    51481 accacaggaa taacacgttg taactcttct tcaacgctaa cttccgccgc ccctggacgt
    51541 gtggactcgc caccaacgtc aatgatcgtc gcgccagcgt tgatcatcag attcgcatgt
    51601 ttcaccgcat ctatcagcga gttatgcgtg ccaccatccg aaaaggaatc aggcgtgacg
    51661 ttgaggatcc ccattacgtg aggatggcta aggtccagtg aagtaccctg ggcaaagagt
    51721 ttcataatgt tatccctggt atgaattgat aagaaaaaac cccggagcac gccccggggt
    51781 ttttggtaca aatacagtca tctgatgcgg gaacttactt gtcgcctaac tgctctgaca
    51841 tggtgttacc cgggttcggc gtacgcggtt catcaaccgg acgaggagcc tttggactac
    51901 cattgtcgcc agaattgtta gaagcgcctg gttcttccca gcccgctggc ggacgtacat
    51961 cgcgacgtgc catcaggtca tcaatctgcg gtgcgtcgat agtctcatat ttcatgagag
    52021 catctttcat cgcatgcagt atatccatat tgtcggtcag aagctgacgc gcacgattat
    52081 agttgcgctc aatcagtgct ttcacttcct ggtcgatgat acgtgcagtt tcatcggaca
    52141 tatgtttcgc cttcgctacg ctacggccga ggaacacttc accttcttct tccgcgtaca
    52201 gcagtggacc caatttctca gagaagcccc actgagtcac catgttacgt gccaggttgg
    52261 tcgcaacttt aatatcgttg gacgcaccgg tagaaacatg ttccggcccg tagatgatct
    52321 cttctgccag acgaccaccg tacagcgtag aaatctggct ttccagtttc tgacggctag
    52381 cgctgattgc gtcgccctca ggcaagaaga aagtcacacc cagcgcacga ccgcgtggga
    52441 taatcgtcac tttgtgcacc ggatcatgtt ccggcaccag gcgaccgata atcgcatgac
    52501 ccgcttcgtg gtaagccgtc gattctttct gcgcttccgt catcaccatg gagcgacgtt
    52561 ccgcacccat catgattttg tctttcgctt tctcgaactc aaccatcgac acaacgcgtt
    52621 tgttgccacg agcagcgaac agtgccgctt cgttcaccag gttcgccagg tcagcaccgg
    52681 agaaaccagg agtaccacgg gcaatgattg ccgcgtcgat atcgggtgcc aatggtacgc
    52741 gacgcatgtg aactttcagg atctgttcac gaccgcgaac atctggcaag ccaaccacaa
    52801 cctgacggtc gaaacggcca ggacgcagca gcgccgggtc gagaacgtcc ggacggttag
    52861 tcgcggcgat aacgatgata ccttcgttac cttcgaagcc atccatctca accagcatct
    52921 ggttcagagt ctgttcacgt tcatcgtgac caccgcccag acctgcgcca cgctggcggc
    52981 ctacggcgtc gatttcatcg ataaagatga tgcacggtgc cgctttcttc gcctgttcga
    53041 acatgtcacg aacacgggat gcacccacac cgacgaacat ttctacgaag tcagaaccgg
    53101 agatagtaaa gaacggaact ttcgcttcgc ctgcaatcgc tttcgccagc aacgttttac
    53161 cggtacccgg aggaccgacc atcaggacgc ctttcgggat cttaccgccg agtttctgga
    53221 agcggctcgg ctcgcgcaga tactcaacca gttcagcaac ttcttctttt gcttcgtcgc
    53281 agcccgcaac gtcagcaaag gtcgttttga tctgatcttc agtcagcatg cgcgctttgc
    53341 tcttaccaaa cgacatggca cctttgccac cgccgccctg catttgacgc atgaagaaga
    53401 tccagacacc aatcagcaac agcatcggga accaggagat gaagatagaa gccagcaggc
    53461 ttggttcttc aggcggttca ccgacaacct tgacgttctt ggtcaacaag ttatccagta
    53521 atttcggatc ctgaaccgga atgtaagtgg tataacggtt actatctttc ttggtaacgt
    53581 tgatttcacg tccgttgata cgcgcttcac gaacctggtc gttattgacc tcttgtagga
    53641 aggtagagta atccacctta cggccattag actcgctggg cccaaagctc tggaatactg
    53701 acatcagcac aacggcaatg accagccaga gtattaggtt tttcgccatg tcactcaagg
    53761 gattaacctc ttattacaac tgtgttaaaa acagcgtcag gatactctat atccagcatc
    53821 tttcaaactt tcgtctgaaa tctcccggtt agggtttacg cccggtcgct acaatataca
    53881 cttcccgcga acgtgcgcga gaagagtccg gcttacgaac tttgaccttc gtaaacaggg
    53941 agcgaatttc ccttagatac tcatcgaaac cttcgccctg gaacaccttc actacaaaac
    54001 tgccacctgg cgctaataca tcacgacaca tttctagcgc cagttccacc agatacatgg
    54061 cacgggggat atccaccgcc ggtgttccgc tcatgtttgg tgccatatcg gacatgacaa
    54121 cctggacttt gctgtcgcca acgcgctcca gcagtgcttt catcaccagt tcatcacgaa
    54181 aatcgccctg aagaaagtcc acaccaacga taggatccat aggtaaaaga tcgcaagcga
    54241 tgatacggcc tttaccgcca atttgggtga ccacatattg tgaccaacca cccggcgcag
    54301 caccaaggtc gacaaccgtc attcccggtt taaagagttt gtcactttgc tgtatttcat
    54361 caagtttaaa ccaggcacgg gaacgtaacc cctttttctg tgcctgttga acatatttat
    54421 cgctaaagtg ttcctgaagc cagcggctgg agctggcaga acgcttctta cctgtcattt
    54481 aactttccca ttgggataac tcatcgtaac caattgcgta aatttttacg cgcctatttg
    54541 gcgatatatg ggagatggcg gtagaatgac ccgttttcaa tcccaacgta agcaaaaaat
    54601 acgatgaatc tgagtactaa acaaaaacag cacctgaaag gtctggcaca tccgctcaag
    54661 ccagttgttc tgcttggcag taatggtttg accgaagggg tgctggccga gattgaacaa
    54721 gcgttagagc accatgaact catcaaggtg aaaatcgcca ccgaagatcg cgaaactaaa
    54781 accttgatcg tggaagctat cgtgcgcgaa accggcgcct gtaatgtaca ggtcatcggt
    54841 aaaacgctgg tgctttatcg cccaactaaa gaacgtaaaa tctcgctgcc acgctaagat
    54901 tatcctaaag ttacacacat ttgctgtgta aaacgagggg ttttccgcag gcaggagagc
    54961 aaaatgccac gctctgttcg ttgataaaag gccgcatagc ggcctttttc ctttctttac
    55021 aatacatcaa catcttgagt attgggtaat tcttacaggt attccacctt aattacttca
    55081 aattctactt cgccgcccgg cgttttgatg accacaacat catcttcttc tttgccgatc
    55141 aggccacgag caataggcga gtttacagaa atcaggtttt gtttaaagtc agcttcgtca
    55201 tcgccaacga tgcgataagt ctgttcttcg tcagaatcca gattcagcac cgttacggta
    55261 gcaccaaaaa taacgcgccc attgttgggc attttggtga catcaatcac ctgcgcgttc
    55321 gacagcttgg cttcgatgtc tttgatacgg ccttcgcaga agccctgctg ttcacgagct
    55381 gcgtggtatt cggcgttttc tttcaggtcg ccatgctcac gcgcttccgc gatagcagca
    55441 atgatttcag gacggcgcac agatttcaga aaatccagct cttcgcgtaa tttttcagcg
    55501 ccgcgtaagg tcatcggaat agcttgcatt tgttatacct cttgaatatt cctgatgggg
    55561 caagtcttca cccaccccgg ctgttcggcc tgcccggcat aactcctcac cgggaccaga
    55621 agcaaaaaaa taccgacccg ggtacaagtc ccaggtcagc tacaattcac attttgatag
    55681 tcattttacc ctgaagttcc cgaagggtca tcgtttactt tatagggcgt tgcgccgtag
    55741 tatgacggct cgattccagg ttgttagcgc gagattatgc gattttccag atttatcatc
    55801 ggattgacca gctgtatagc gttcagtgtt caggccgcaa atgttgatga gtacattact
    55861 caactccccg ctggtgccaa ccttgccctg atggtgcaaa aagtcggcgc gtcggccccc
    55921 gctattgatt accacagtca gcagatggcg ctgcccgcca gtacgcagaa agtgattact
    55981 gcactggcgg cgttgattca actcggcccc gattttcgtt ttaccacgac gcttgaaacc
    56041 aaaggcaatg tggaaaacgg cgtacttaag ggtgacttag tggcgcgatt tggtgccgat
    56101 ccgacgttaa gacgtcagga tattcgcaat atggtcgcga ctttgaaaaa atctggcgtc
    56161 aaccaaatcg atggcaatgt gttgatagat acctccattt tcgccagcca cgataaagcc
    56221 cccggctggc catggaatga catgacacaa tgctttagcg ctccgcctgc cgccgccata
    56281 gttgaccgca actgtttctc cgtctcgctc tatagtgccc caaaacctgg tgatatggct
    56341 tttatacgcg tggcatctta ttaccccgtt acgatgttca gccaggtacg caccctcccc
    56401 cgtggttctg ccgaagcgca atactgcgag ctggatgtgg tgccaggcga cctgaaccgc
    56461 tttacgctga ccggatgcct gccacaacgt tctgagccgc tcccgttggc ttttgccgtg
    56521 caggatggtg ccagctatgc tggagcaatt ctgaaagatg agttaaaaca ggcaggtatc
    56581 acctggagcg gaacactgct gcgtcagacg caggttaacg aacccggaac ggtagttgcc
    56641 agtaaacagt cggccccgtt gcacgatctg cttaagatta tgctgaaaaa gtcggacaac
    56701 atgattgccg atacggtttt ccgcatgatc ggccatgcgc gcttcaatgt gcctggaaca
    56761 tggcgggccg ggtcggacgc cgtgcgtcag atcctgcgcc agcaagccgg tgtcgatatt
    56821 ggaaatacca ttattgccga tggttcaggg ctttcgcggc ataacctgat tgctcccgcc
    56881 accatgatgc aggtgctgca atacattgcc caacacgaca atgaacttaa ctttatctct
    56941 atgctgccgc tggcgggcta tgacggctct ttgcagtacc gtgcaggtct gcatcaggcg
    57001 ggcgtggatg gaaaagtctc agcgaaaacc ggttcgttgc agggggtata taacctggcg
    57061 ggattcatta ccacagcgag cgggcaacga atggcgtttg tgcaatatct ttctggctat
    57121 gcagtagaac cggcggatca gcgtaatcgc cgtattccgt tggtacgttt tgaaagccgt
    57181 ttgtataagg atatttacca gaacaattag tctaaagaga aaccccggca catggccggg
    57241 gttttgctga ttaacgcttg taaatgaact caacgccttc ttcgtcgtct tcgtcccagt
    57301 cgtcatccca gtcttcatca tcctcttcag caatctcttc aagctgctgg cgatgataat
    57361 catcccacat gaattcgact ttctctggct gtttcgcttc ttcagcctgc acgaccgggt
    57421 tttcaatgat aaaggtcatc acatcccagc agagatcttt cacgcccagt ccgctcgccg
    57481 cagagatcag ataatattta tcttcccaac ccagcgcctc agcgatcgct ttcgctttct
    57541 cttcggcttc tgccttatcc agcaggtcga tcttgttgaa gactaaccaa cgcggtttcg
    57601 ccgccagatc ctggctgtat ttttccagct cgctgataat aatacgcgcg ttttcaaccg
    57661 gatcggtgcc atcaatcgga tcgatatcga tgaggtgcaa caggacgcgg caacgctcca
    57721 ggtgcttcag gaagcgaatg cccagacctg cgccttctgc agcgccttca atcagtcctg
    57781 gaatatcggc aacaacgaag ctcttttcgt tgtccattcg taccacacca agacttggca
    57841 ccagagtggt aaacggataa tccgccactt tcggtttagc cgccgatact gcgcggataa
    57901 atgtcgattt acccgcgttt ggcatcccca gcataccgac gtcagccagc agcatcagct
    57961 ccagtagcag ctcacgctta tcgcccggcg taccgttagt tttctgccgt ggtgtacggt
    58021 taactgacga tttgaaacgg gtattgccca gaccgtgcca gccgccctta gcaaccagca
    58081 gacgctgacc gtgtttggtc atatcgccca tggtttcacc ggtaccctgg tcgataacac
    58141 gcgtacctac cggcacttta atcgtcacgt ctttaccgcg cttaccggta cagtcgcggc
    58201 ttgcgccatt ctgaccacgc tctgcgcgga aagatttttc aaaacgataa tcgataagcg
    58261 tgttcaggtt ctcgtcggct tccatccata cgtcaccgcc gtcaccgccg tcgccgccat
    58321 ccgggccgcc tttcggaata tacttttcgc ggcggaagct cacgcaacca ttaccgccat
    58381 cacctgcaac gaccagaatc gatgcttcat caacaaactt cattttattc tccgtaaatc
    58441 attcgcctgc gcggggttgc gataccaccg ttgtatgctt acgtaatccg ccccaaatac
    58501 gatgaccaat ggcggaatac atcgcgcccg caaccacgac aaacgcaccg agataaccta
    58561 aaaggtttaa catcggtctg gcgaagaaat cgggccaggc cagtgataaa agatctgaaa
    58621 aaaacagcgt aaacagtggg gtgagcgtaa tgatcgcgct cacctgcgct gcctgccagc
    58681 gagccatcgc ttccgccagg gcgccatatc ctaccaatgt attcagtccg caaaaaatta
    58741 aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc aggctttgcc agagggaaga
    58801 gcgcaattgt acataaagtg tacagcaaaa acaggatctg cggtgaggcc agtcgacgca
    58861 ataaaacctt ttgcgccacg ccataactca cccacaccgt cgccgcaccg accccaaaga
    58921 ttactcccca ggtgtaatcg gtgagctttg taaatatctc gaccagactg gtgttaaaaa
    58981 acatcaccag gccgctcagg agcatcaatg cccctacaac ctgagtgctg cgcattttct
    59041 ctttcaggat aaatacgctg gcaaccatca tgccaactgg cgagagttgc ccaatcacct
    59101 gcgaagcggt cggacccagg tattgcaagg atgagctgaa caggatgaag ttcccaaaca
    59161 gcccggcggt cgccactgcc aacaaaatca accagcgtgg cttacgaaac acgcgtaatg
    59221 gcggcaaccg cttcttcacc gcaagaatgg cacccaggcc aatactcgcc atcaagaaac
    59281 ggtaaaacac gattgtcgga ggttccatca cctccagcac ctgcttcatt gcgattggca
    59341 acgcccccca gcaaattgct gtggtgagcg ccaaaagaat gccaatgcct gcctgctgct
    59401 tcataccgta ttccctgcaa agcgcatttg cggattaacg tcgcaccatg cgcattttta
    59461 ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg gctttcatcc gttaccggga
    59521 cgcgaaaaac ttattcagct tcgatgctga taaatttacg gtttttcggg cctttaactt
    59581 cgaatttcac tttaccgtct gctttagcaa acagagtgtg gtcacgaccg caacctacgt
    59641 tagcgccagc gtggaatttg gtaccacgtt gacgaacgat gatgctaccc gccagaacgg
    59701 attcgccacc gaaacgctta acgcccaggc gtttagcttc tgaatcgcga ccgttacgtg
    59761 tggagccgcc agccttttta tgtgccattt gaaatctctc ctcaggtctt aggcgctgat
    59821 gccagtaatt ttcacatcag tgaaccactg acgatggccc tgctgcttac gatagtgttt
    59881 acgacgacga aacttaacga ttttaacttt ctcgccacga ccgtgagcaa caacttcagc
    59941 tttgattacg ccgccatcaa cgaaaggaac gccgattgtg acttcttcac cgtttgcgat
    60001 catcagcact tcagcgaact caacagtttc gccagttgcg atgtccagct tttccaggcg
    60061 aacggtctga ccttcgctta ctcggtgttg tttaccacca ctttggaaaa ccgcgtacat
    60121 ataaaactcc gcttccgcgc acaccttttc aatgattcag agtgcgctat aaatattcac
    60181 aatagggcgc gaatattacg caaaacgcac gcctttgaca agtgctacag tcaatacacg
    60241 aagaaaaaaa acacaacttg tacggtaacg tttatctgtg ccattttttc agtacaatca
    60301 ccctatattc ctaaccataa accctaagtt gcctttgttc acagtaaggt aatcggggcg
    60361 aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg agttaaccgc gcaagatatg
    60421 gcgggtgtta atgcggcaat ccttgagcag cttaattccg acgtccaact gatcaatcag
    60481 ttaggctatt acatcgtcag cggcggcggt aaacgtattc gtccgatgat tgctgtactg
    60541 gctgcacgag ctgttggcta tgagggaaat gcgcatgtca ccattgctgc cctgatcgag
    60601 tttatccaca cggcgactct gctgcacgac gacgttgtgg atgaatcaga tatgcgcagg
    60661 ggtaaagcta ccgccaacgc tgcatttggc aatgccgcca gcgtgctggt aggcgatttt
    60721 atttataccc gcgcgttcca gatgatgacc agcctcggct cgctcaaagt gctggaagtc
    60781 atgtcagaag ccgtaaacgt catcgcagaa ggtgaagttc tgcaactgat gaacgttaac
    60841 gatccggaca tcactgaaga aaactacatg cgcgttatct atagcaaaac cgcgcgtctg
    60901 tttgaggctg ccgcgcagtg ttccgggatt ctggctggct gtacgccgga ggaggagaaa
    60961 ggcctgcagg attatgggcg ctatctcggc actgctttcc agttgatcga cgatttactc
    61021 gattacaatg ccgatggcga acagttaggt aaaaacgtcg gcgacgatct gaacgaaggt
    61081 aaaccgacgc tgccgctgct gcatgcaatg catcatggca caccagaaca ggcacagatg
    61141 attcgtaccg ccatcgaaca gggtaacggt cgccatcttc tggaaccggt tctggaagca
    61201 atgaacgctt gtggatctct tgaatggacg cgtcagcgtg ccgaggaaga agcagacaaa
    61261 gccatcgcag cgttacaggt gctcccggac accccgtggc gagaagcact catcggcctc
    61321 gcgcacatcg ctgttcaacg cgatcgttaa tcccctcccc tcatcccgcg cagcgcgcgg
    61381 gatgattcca gtaaattcca taaaaacact tattcagctc tataacacct gaaaaatagg
    61441 gacgtacatc tttatatatt ctgaatattc acactcttta caggaacttt ttagagcaat
    61501 aggccatcag gagtatagtg atgctcgaca gaagaagtgt tctgaatgaa agcgactaac
    61561 ttaaggagtg aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg
    61621 cgggtttgcg caagaaggga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt
    61681 cctcaacgct ggcgaacgca ttatcgcgcc cctggccgaa aggagagatg attattgcga
    61741 aagccctggg aactgacccc tggattatct ggccatctcg ctaccatgat ccgcagaccc
    61801 atgagtttat cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg
    61861 tctggcggta gccccgcgaa cggggctgcc agctctcaga caattactcg cctttcacac
    61921 gctcaatatt tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga
    61981 tgtgataaat acgatcaact accgtcgtcc cttccgcaat acagccagcc agcaccaggc
    62041 ttgctgatgc acgcagatcg gttgccataa cctgtgcgcc agaaagtttt tcaacaccgt
    62101 gacaaataac ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca
    62161 catgcataaa gcggttttca aagaccgttt cggtgatgaa gccggtccct tctgccacca
    62221 ggttcaacag cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac
    62281 gtacgttaac agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga
    62341 cttcgatgtc tgctccagcg tcacgcagtt tcgccagcac ggcgtcgaga gtatctggct
    62401 gcgcgttacg gcagataatt ttgccgcgag agatcgccgc cgccaccagg aaagtaccgg
    62461 tttcgatacg atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga
    62521 tgacgatacg atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg
    62581 cggtatcgac gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca
    62641 gggttgcagc acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat
    62701 gtgcgccttt cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg
    62761 tcgcgcctaa ttgttcgaga ccagaaatgt gtagatcaac cggacgtgca ccaatcgtac
    62821 aaccgccagg cagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc
    62881 agatagaagc acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc
    62941 gggcatcaat atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagct
    63001 tcattgatgt atcgacgtct tttagtttcg ggacgttctg gatctctacc ggttcttccg
    63061 ccagtagtgc ggcaaaaagg ataggcagag cagcattttt agcgccggaa attgtgactt
    63121 cgccctggag cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt
    63181 aacaattcat atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat
    63241 cgcgcgccca ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg
    63301 caatatattc catcagcggg ccatagaccg tctgctgttt tttaacccga ctcatgccgt
    63361 caaacaactc acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt
    63421 cctggaggga gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt
    63481 caatcatcag ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa
    63541 gcaaaaagcc tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag
    63601 gcggttaacg ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat
    63661 tatacaattt cgccagggta tacactttgt cgtttacccc ctgaagcgtc acattgttgc
    63721 cctgcttttt cgccagatcg ataagatgga gcagcagcgc cagtccacct gtatcaacgc
    63781 gggatacacg gctaagatcg atacaggtaa tccccttcac cgcttcctca cgcatttccc
    63841 aaagcggtag cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct
    63901 gcatccagct cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga
    63961 aatcgatttc agttgcgcag tcaggccgtc aatacctttg gtacgcagca gcgttcccca
    64021 ctcgttttgt ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcctgcca
    64081 gttgcccgtc tgggagtttt tacgccactg gaagtccaga cgcaccggcg ggcggccatt
    64141 cgggtcaata atggtaacgc gaataggcac aatggtttta tcacccagcg gctgttctgg
    64201 cgcaatctga taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag
    64261 gtactcacgg aaagcggcaa agtaagcatc acgttgagct ggggtcgcac tcttgtaata
    64321 ctgacccagc accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc
    64381 aacaatggta cgcagataat ccgggttggc ccgaatttgt ggttgctcat tcttcaggcg
    64441 atcgaacgtt ttctgcgccg cctcgtccat cagcttatac ggattggtct ggtctgccgc
    64501 ggttgccgca ctcagcggtg caatcaccag caaagcgacc atcattaaac gtttaaacat
    64561 gcgtcggttc tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc
    64621 tggcgcagca gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag
    64681 gaactgacca ataagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc
    64741 atccttcagg atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata
    64801 ttgttccccc agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt
    64861 ataacgttgt tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat
    64921 atccgccacc cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc
    64981 gccaatgtta tcgaacgtcg cataaagcgt gtaggtcggt tcagtacgta tggacgtcac
    65041 gttcgccgcc ttcaggcaaa caaacagcgc cgccagtagt gctgctaata aaaagatacc
    65101 cacccaaatt tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca
    65161 gcacaaaatc cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc
    65221 ggctaatccc ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca
    65281 ccgtgatggc aaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga
    65341 cggcattttg cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga
    65401 ccagagatcc gccccagatc cccacggcga cgaaaataac cgtcaacagt ggtaatgaaa
    65461 taaccccagc ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct
    65521 ccatactgga gagttgctct gtagcgcgca tcaggccgat ttctgcggtt agcgccgaac
    65581 cagcacgccc ggcaaacaac aacgcggcaa ccaccggccc cagttcacgc agtagcgata
    65641 acgccaccag catacccaga ctggtttccg cactataagt ggtcagaacc agataacctt
    65701 gcagccccaa caccattccg atgaacacgc cagaaaccac aataatcagc atcgacagga
    65761 cgccgacatt atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc
    65821 cgaccagcgc attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc
    65881 ctttatgtcc gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa
    65941 agatcagcgt gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg
    66001 tccagaaact gacgtacgcg tggatcagga ttcgcctgca acgcctgggc actgccatga
    66061 gcgacaattt ttttgtccgc caggatccag gcgtgatcgg caatacttaa cacttccggc
    66121 acatcgtgag aaaccaccac acaagtcaca cccagcgcgc tgttcagctc agaaatcagc
    66181 ttcaccagta cgcccatggt gatgggatct tgcccaacaa agggttcatc aaacatgatg
    66241 agatccggct ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa
    66301 agttcagaag gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc
    66361 accgtactat gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg
    66421 ttgtcaaata cgttcatatc agtgaacaac gcccctgact gaaataacat gctcatccgt
    66481 ttgcgcactg tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaaga
    66541 atctcaccat gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg
    66601 atgcccgatg gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta
    66661 tcgaagatgc agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc
    66721 acagactgct ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat
    66781 ttaaccttga acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca
    66841 tttgttttac tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa
    66901 atcttcagcg gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc
    66961 actgttaatt gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc
    67021 gtctattctt tgccgaacct ttggcatccc gccgctaatc atcggcatga cggtggtcag
    67081 tattggtaca tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg
    67141 cgatttagcc gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg
    67201 tctggctgcg ctggttcgtc cttttaccgt ccattctgat gttctacgcc gtgaattacc
    67261 cttaatgttg ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg
    67321 aagcgatggt atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact
    67381 tgcacgtcag gctgaacgtc aggggactga cagcctgacc agagagcagc ttgcagagct
    67441 gcctcgtgac ggcggattgc ccgtcgcgtt tttatggctc ggcattgcgc ttatcatcat
    67501 gccagtggcc acgcggatgg tggttgataa cgccacggtg ctggcgaatt actttgccat
    67561 cagcgagttg acgatgggtc tgacggcaat tgctatcgga accagcctgc cggaactggc
    67621 aaccgcaata gcgggggttc gcaaaggtga aaacgacatt gctgtcggaa atatcattgg
    67681 cgcaaacatt tttaatattg tcatcgtatt gggtttaccc gcgctgataa cgccaggaga
    67741 gattgatcca ctggcgtaca gtcgtgacta cagcgtgatg ttgctggtga gcattatttt
    67801 tgcgttgctg tgctggcggc gctccccgca accgggccgt ggtgtagggg tattattaac
    67861 tggcggattt atcgtatggc tggcgatgtt gtactggtta tcgccaatac tcgttgaata
    67921 actggaaacg cattatgtcg cacgtagagt tacaaccggg ttttgacttt cagcaagcag
    67981 gtaaagaagt cctggcgatt gaacgtgaat gcctggcgga gcttgatcaa tacatcaatc
    68041 agaatttcac gcttgcctgt gaaaagatgt tctggtgtaa agggaaagtt gtcgtcatgg
    68101 ggatggggaa atcggggcac atcgggcgca aaatggccgc aacgtttgcc agcaccggta
    68161 caccttcatt tttcgtccat cctggtgaag ccgcgcatgg tgatttaggc atggtcaccc
    68221 cacaggatgt ggtgattgct atctctaact caggtgaatc cagcgaaatc acggccttaa
    68281 ttccagtgct taagcgtctt cacgtaccgt taatctgcat caccggtcgc ccggagagca
    68341 gcatggcgcg cgccgcagat gtgcatctgt gtgttaaagt agcgaaagaa gcctgtccgt
    68401 tagggctggc accgaccagc agcaccaccg ccacgctggt tatgggcgat gccctcgctg
    68461 tcgcgctgtt aaaagcacgc ggctttactg ctgaagattt tgcgctctca cacccaggcg
    68521 gcgcactggg tcgtaaactt ctgctgcgcg taaacgatat tatgcatacg ggcgatgaga
    68581 tcccgcatgt taagaaaacg gccagtctgc gtgacgcatt gctggaagtt acccgcaaaa
    68641 atcttggtgt gactgtcatt tgcgatgaca atatgatgat tgaaggcatc tttaccgacg
    68701 gtgatttacg ccgtgtcttc gatatgggcg tggatgttcg tcagttaagt attgccgatg
    68761 tgatgacgcc ggggggaata cgtgtgcgcc ctggcattct ggccgttgag gcactgaact
    68821 taatgcagtc ccgccatatc acctccgtga tggttgccga tggcgaccat ttactcggtg
    68881 tgttacatat gcatgattta ctgcgtgcag gcgtagtgta aagattcaag gataaacaac
    68941 aatgagcaaa gcaggtgcgt cgcttgcgac ctgttacggc cctgtcagcg ccgacgttat
    69001 agcaaaagca gagaacattc gtctgctgat cctcgatgtc gatggcgtac tgtcagatgg
    69061 cctgatttat atgggcaata atggcgaaga gctgaaagcg ttcaatgttc gtgacggtta
    69121 tggcattcgt tgtgcgctca cctctgatat tgaagtcgct atcattaccg ggcgaaaggc
    69181 taaactggta gaagatcgtt gtgccacatt ggggatcact cacttgtatc aggggcagtc
    69241 aaacaaactg atcgccttta gcgatttgct ggaaaaactg gcgattgccc cggaaaatgt
    69301 ggcttatgtc ggcgatgatc tcatcgactg gccggtaatg gaaaaagtgg gtttaagcat
    69361 cgccgtggcc gatgcgcatc cactgttaat cccgcgcgcc gattacgtga cgcgcattgc
    69421 tggcggtcgt ggtgcagtgc gtgaagtttg cgacttatta ctcctggcgc agggcaaact
    69481 ggatgaagcc aaagggcaat cgatatgagt aaagccagac gttgggttat cattgtgctg
    69541 tcactggcgg ttctggtgat gatcggcatt aatatggccg aaaaagacga taccgcccag
    69601 gtggtcgtca acaacaatga tcccacctat aaaagcgagc atacggacac gctcgtctat
    69661 aacccagaag gggcactaag ctatcgattg attgctcaac acgttgaata ttattccgat
    69721 caggccgttt cgtggtttac gcagccggta cttaccacgt ttgataagga taaaatcccg
    69781 acatggtccg taaaagcaga taaagccaag ctgaccaatg accggatgct ctatttatat
    69841 ggacacgttg aagtcaacgc actcgtgccg gactctcaac ttcgcagaat cacgacggat
    69901 aacgcgcaga tcaatctggt gacgcaggat gttacctctg aagacctcgt cacgttatac
    69961 ggaacaacat ttaactccag cggtctgaaa atgcgcggca acttacgcag caagaacgcc
    70021 gagctgattg aaaaggttag aacatcctat gaaattcaaa acaaacaaac tcagccttaa
    70081 tcttgtgctt gccagctcac ttctggccgc cagcattccg gcatttgccg taaccggaga
    70141 cactgatcag ccgatccaca ttgaatcgga ccagcaatct cttgatatgc aaggcaacgt
    70201 ggttaccttt accggcaatg tcatcgtcac ccagggcacc atcaaaatta atgccgacaa
    70261 agtggtcgtt acccgtccgg gcggcgaaca aggtaaagaa gtgattgacg gctacggtaa
    70321 accggcaacg ttctaccaga tgcaggacaa cggtaaaccc gttgaaggtc acgcttcaca
    70381 gatgcactac gaactggcaa aagattttgt cgttctgacg ggtaatgctt atctgcagca
    70441 ggtcgatagc aacattaagg gcgataagat cacttacctg gtgaaagagc agaaaatgca
    70501 ggctttcagc gacaaaggca agcgcgtaac caccgttctg gtgccgtcgc agctgcagga
    70561 caaaaacaat aaaggccaga ccccggcaca gaagaagggt aattaattcg ttatggcaac
    70621 attaactgca aagaaccttg caaaagccta taaaggccgt cgcgtggtag aagacgtcag
    70681 cctgaccgtc aactccgggg aaattgtcgg tctgctgggg ccaaacggtg ccggtaagac
    70741 caccactttc tacatggttg ttggcattgt gccgcgcgat gcgggcaaca tcattattga
    70801 tgatgacgat atcagtctgc tgcctctgca tgcacgcgcg cgccgcggta tcggctatct
    70861 gccgcaggaa gcctccattt tccgtcgcct cagcgtttac gataacctga tggcggtact
    70921 gcaaattcgt gacgacttgt ctgctgaaca acgtgaagac cgcgcgaacg agctgatgga
    70981 agagtttcac attgagcacc tgcgtgacag catggggcag tcactctccg ggggtgaacg
    71041 tcgccgtgta gaaattgccc gtgcactggc tgcgaatccg aaatttattc tgctcgacga
    71101 accgtttgcc ggggttgacc cgatctcggt tatcgacatt aaacgcatca ttgagcacct
    71161 gcgcgacagc ggcctgggcg tactgatcac tgaccacaac gtgcgtgaaa cactggcggt
    71221 ttgtgaacgc gcttatatcg tcagtcaggg gcatttgatc gcccacggca cgcctacaga
    71281 aatcttacaa gacgaacacg ttaagcgtgt ataccttggg gaagacttca gactctgata
    71341 gggtagaagt ttgcgatgtt ttagcaggag agtacgattc tgaacatgaa gcaaggtttg
    71401 caactcaggc ttagccaaca actggcgatg acgccacagc tccaacaggc aattcgtctg
    71461 ttgcagttgt cgacgctgga acttcagcag gagctacagc aggcgctgga gagtaatccg
    71521 ctgcttgagc aaatcgacac tcatgaagaa atcgacaccc gcgaaacgca agacagtgaa
    71581 acactggaca ccgccgacgc gctcgaacaa aaagagatgc cggaagagct gccgctcgat
    71641 gccagttggg acaccattta caccgctggt acaccatccg gcaccagcgg tgactacatt
    71701 gacgacgagc tgccggtcta tcagggcgaa acgacgcaga ccttgcagga ttacctgatg
    71761 tggcaggtcg agctgacacc gttttccgac actgaccgcg ctattgctac ctctatcgtc
    71821 gatgccgttg atgacaccgg ttatctgact gtcccgctgg aagatattct cgaaagtatg
    71881 ggcgatgaag agattgacat cgacgaagtt gaagccgtcc ttaagcggat ccaacggttt
    71941 gatccggtcg gtgtggcggc aaaagatctg cgtgactgtc tgctgatcca actctcccaa
    72001 ttcgataaaa ccacgccatg gctggaagag gccagactga tcattagcga tcatctcgat
    72061 ctgttagcca atcacgactt ccgcacttta atgcgcgtca cgcggctgaa agaagatgtg
    72121 ctgaaagaag ccgtcaatct gatccagtcg ctcgatccgc gccccgggca gtcgatccag
    72181 acgggcgaac ctgagtatgt cattccagat gtgctggtgc gtaagcataa cggtcactgg
    72241 acggtagaac tcaacagtga cagcattccg cgtctgcaaa tcaaccagca ctacgcctcg
    72301 atgtgcaata acgcgcgcaa cgatggtgac agccagttta tccgcagcaa tctgcaggat
    72361 gccaaatggt tgatcaagag cctggaaagc cgtaacgata cgctactgcg cgtgagtcgc
    72421 tgtatcgttg aacagcagca agccttcttt gagcaaggcg aagaatatat gaaaccgatg
    72481 gtactggccg atatcgccca ggctgtcgaa atgcatgaat cgacgatatc tcgcgtgacc
    72541 acgcaaaaat acctgcatag tccacgaggc atttttgaac tgaagtattt cttttccagt
    72601 cacgtcaata ccgagggcgg cggcgaagct tcctccacgg cgattcgtgc gctggtgaag
    72661 aaattaatcg cggcggaaaa cccagcgaaa ccgttgagcg acagcaagtt aacctctttg
    72721 ctgtcggaac aaggtatcat ggtggcacgc cgcactgttg cgaagtaccg agagtcttta
    72781 tccattccgc cgtcaaacca gcgtaaacag ctcgtttgac ccaaccgata aggaagacac
    72841 tatgcagctc aacattaccg gaaataacgt cgagatcacc gaggcactgc gcgaatttgt
    72901 tacagccaaa tttgccaaac ttgagcaata ttttgaccga atcaaccagg tctatgttgt
    72961 tctgaaagtg gagaaagtca cccacacctc agatgcaaca ctgcatgtaa acggtggtga
    73021 aattcatgcc agcgcggaag gtcaggacat gtacgctgcc attgatggct taattgataa
    73081 gctggcacgc cagttgacca aacataaaga taaactgaaa caacactaat tgtccgggca
    73141 attagcatgt gcatggcggc ctgttgtgcg gcacaacggg ccatttgtac ggttaatgct
    73201 ccgagcctgt tccactgttt gagtgggcag gttcttaggt gaaattatga caaataatga
    73261 tacaactcta cagcttagca gtgttcttaa cagggaatgt acgcgaagcc gcgtccactg
    73321 tcagagcaaa aaacgcgccc tggaaatcat cagcgagctg gcggcgaaac aacttagtct
    73381 gccgcctcag gtggtttttg aagctatcct gacgcgggaa aaaatgggca gtaccggtat
    73441 cggcaatggt attgccattc cgcatggcaa actggaagaa gatactctgc gcgccgttgg
    73501 cgttttcgtt cagctcgaaa cgcctatagc tttcgatgcc attgacaacc aaccggtaga
    73561 tttactattt gcgttgctgg ttccggctga ccaaaccaaa acgcacctgc atactctgtc
    73621 gctggtggcg aaacgtctgg cggacaaaac catctgccgc cgtttacgcg cagcccagag
    73681 cgatgaagag ctgtatcaaa tcattacgga taccgaaggt actccggatg aagcgtagtt
    73741 attcggtaat gtctctttta gacgttgtga ggagaaacag tacatggtac tgatgatcgt
    73801 cagcggacgt tcaggttcag gtaaatctgt cgccctgcgt gcgctggaag atatgggttt
    73861 ttactgcgtg gataaccttc ccgtagtgtt gttacccgat ctggctcgaa ccctggccga
    73921 tcgcgagatt tctgccgccg tcagcattga tgttcgtaat atgccggagt caccagaaat
    73981 attcgaacag gcgatgagta acctgcctga cgctttctca ccgcaactcc tgttcctgga
    74041 tgccgaccgt aataccttaa ttcgtcgtta cagtgacacg cgccgactgc atccgctttc
    74101 cagcaaaaac ctgtcgctgg aaagtgctat cgacaaagaa agcgatttgc tggagcctct
    74161 gcgttcgcga gcggatctga ttgtcgatac ctcagaaatg tccgttcacg agctggcaga
    74221 aatgctgcgt acccgtctgc tgggtaaacg tgaacgtgaa ctgaccatgg tctttgagtc
    74281 tttcggcttc aaacacggta tccctatcga tgcagattac gtctttgacg tgcgcttctt
    74341 gccgaacccg cactgggatc cgaaactgcg tccaatgaca ggtcttgata aacctgtcgc
    74401 cgcgttcctc gaccgccaca cagaagtaca caattttatc taccagacgc gaagctatct
    74461 tgagctatgg ttacctatgc tggaaaccaa caaccgtagc tacctgacgg tcgccattgg
    74521 ttgtaccggc gggaagcacc gttcggtgta tattgcagag caactggcag actacttccg
    74581 ctcgcgcggt aaaaacgtcc agtcacgcca tcgtacgctg gaaaaacgta aaccatgacc
    74641 gtcaagcaaa ctgttgaaat cacaaacaag ctgggcatgc atgcccggcc tgcaatgaag
    74701 ctgtttgaat taatgcaagg ttttgacgca gaagtgctgt tacgtaatga cgaaggtacc
    74761 gaggccgaag ccaacagcgt tattgcactg ctgatgctgg attctgccaa aggacggcag
    74821 attgaagttg aagcgaccgg tccacaggaa gaggaagcac tggccgccgt tatcgccctc
    74881 tttaattctg gttttgatga agattaatct tcatcacttt gacattcaaa tcccttcaag
    74941 ctcccccgac aataagaaaa gcgcgtgctg aaatcgttct caatcaacgt catttgtaca
    75001 ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac cttaagaagc attgtttagg
    75061 tttcgtttaa gttaatcgac catactggag atcgtcagaa aatatttcca ggagatggca
    75121 tgattcgctt atctgaacaa agtcccctgg gcaccggaag acatcggaaa tgttatgcgc
    75181 atccggaaga tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata
    75241 aagagatccg ccgtgagtta aagtactacg cgcatcttgg tcgccggtta aaagactgga
    75301 gtggaatacc gcgctatcac ggtaccgttg agacggattg cggaaccggt tatgtctacg
    75361 atgtgattgc agattttgac ggcaaaccct ccattacgct gaccgaattt gctgaacaat
    75421 gccgttacga agaagacatt gcacaactgc gccagctact gaaacagctg aagcgttatt
    75481 tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca
    75541 tcagtgagtc agaggttatc cccgtcgtct gcgacaacat tggtgaaagc acattgattc
    75601 cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta tggaaacgat
    75661 ttatcgccca accggcgctg gctattgccc tgcagaaaga tctgcaacct cgtgagagca
    75721 aaacgctggc cctcacttcg cgcgaagctt aatccagctg gtgctgttgc ataaacgact
    75781 caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg acggctacgc acgtagcccg
    75841 atggcgcgga gactttgaaa cgaagtggat taggtaatac agcagccagt aatgcagctt
    75901 ccgagcgggt aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc
    75961 caaacacacc gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata cgctttttgc
    76021 tccagactgt ttctatcccc agcgttaatc cagcttccag cccttttcgt acccagctac
    76081 gcccatccca taaaaagaga tttttggccg tctgctgaga aatcgttgaa gcaccgcgaa
    76141 tacggttttc attgcgctcg ttgtgcgcca gggctttctc aatggaagcg acatcaaagc
    76201 cccagtgctc aggaaatttc tgatcttctg cggcaatcac cgccagtccc atccacggcg
    76261 agatttgatc catactgacc cagtcagaat gtgccacgta acgaaaattg ccatgcagcc
    76321 aggcgctgac ctgtcgctcg accattaccg ctgagaaggg aacaggcgca acactaaaca
    76381 acgcgatgcc cccgccccag aaaacggcga ggacaaccat taaccgcaaa agaaaacggc
    76441 gaacgaaact aaacaccgtt aagcggcttt tactcattca gccagaacca gcacgcggga
    76501 aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca tatatgctgg
    76561 ggtggtgaca atcttattgt cttcatcaac cacgatatca tcgacaggac atggcacatg
    76621 ctccgcgccc atctcttcca gcacttctgc ggtatcgata tcagtaccga tggtcaaacg
    76681 cagcgggaaa tcgaaaattt tcggcagcat cgccggggca atacacataa aaccaagcgg
    76741 ttttccggcc tgatgcatcg cttgtgccag cgcctttaat tcacggtcaa cggtgcattc
    76801 gctaccaaga ctggcaaaat tgcttaaatt cttcgccgcg ccaaaacccc ccggcacaat
    76861 caacgcatcc agttcagcgg catcggcctg ggccagagga cggatttcac cgcgcgttat
    76921 tcgtgccgct tcaatcagca cattgcgcgt ttccgtcatc gcttcgcccg ttaaatggtt
    76981 gataacatca acctgctgct tatccggtgc aaagcagacc gcctgcgcac cgctgcgtga
    77041 aatagctaac aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga cgccgcatcc
    77101 gctcagaatt acgccaattt tcttcattgt gatcatcctt tcgcaattga ctgaaacaca
    77161 ttaatttttt taataaaaat ggtacgcatc acacatttaa ctgattcatg taacaaatca
    77221 tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc ccttgtaact
    77281 catcataata atttacggcg cagccaagat ttccctggtg ttggcgcagt attcgcgcac
    77341 cccggtctag ccggggtcat tttttagtgg cttttgccac ccacgctttc agcacttcta
    77401 cgtcgtgacg ccactcttct ttcatctctt caatccattc accgacgtta tcttcccagg
    77461 caggaaggtc aggagactga atttgctgac ccagctgttg cagatggcgt aaccccactg
    77521 accccgccgc acctttaatt ttatgtcctt cctcaacaat gcctttttta tcctgcgccg
    77581 tcagattcga ctccagcacg ctgacatagc ccggcatcat cttctcaaac accgctaacc
    77641 cgtcggtgat cagcttcggt cctacaagtg cgagatactg ttccagcatg ggaatatcga
    77701 gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt actctcctca tcatcctggg
    77761 tatcccagaa tttcttgatc atcgcggtta gcgccggaac cgaaagcggc ttactcagca
    77821 catcatccat tccagcattg aggtactctt gtttgtcttt cagcacgtta gcggttaagg
    77881 ccaccagcgg cggtaaatcc tcgcgcggat aacgcttcgt cagttcacga gagatatcca
    77941 gcccggtcat atctggcaac tgaatatcca gcaacaccag gtcgtattcg cccggtttaa
    78001 acatctccag cgccgccttg ccggtcatgg cgacatcaac gctgttacct aatttttcca
    78061 gcacagaacg cgcaacaatc acgttcagtt caatgtcttc caccagcagc acattcagcg
    78121 ccggtaaagg catatcgtct tcatcaaacg catcatcgac ctcttctgct accgacggtg
    78181 cgtggatcgt caacgtaaag gttgaacctt tgccctgttc gctggtaacc gtaatatcgc
    78241 cgcccatatt tttcgccaga cgacgagaaa cggccagacc aataccggtg ccggtggcag
    78301 gtttaccgcc atgactgtct ttcacctggt aatacatggc gaaaatttta tccagctcat
    78361 cctgcggaat gccgatacca gagtcttcca cttcaaaatg cagcatatcg ccttcatcgt
    78421 agcgcacgcg cacggtaacc tggccttgct gggtgaattt gacggcgtta ctgatgagat
    78481 tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt aatggcagcg
    78541 taggctccag gttaaagcgc aatccttttt gttgcgcctg caatgcggaa agattttcca
    78601 gatcggcaag gaagctggtg aaatcaaccg gctgattatc aagctggacc ttacgccgtt
    78661 ccatcttatc catgtcgata atatcgttga agatattccc gagcgtgacg gccgaaacat
    78721 ggatggtttt gagatatttt tcctgctcgg cggtgagttc ggtatccagc agaatgcggc
    78781 tcaggccgac gataccattc agcggtgtac gcaattcgtg actgatggtg gagataaacg
    78841 tcgttttgtc gcggctggcc cgttcaagcg catcctgata ccgcttacgc tcggtaatgt
    78901 cgcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac ggcactttac
    78961 ggatttcaaa gcaggctttg cgtccgtcgg gataatccag ccactgctca taggtcagtg
    79021 acacattatg acggaacact ttttcatcgg tttcaatgac tttcgcagcg gcttccggtg
    79081 agtaaacatc agcaggtttc agatgaacca gttgtttttc gctttttccg gtcagcagct
    79141 ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt acgataaaaa accaggtcgg
    79201 gtgaagcgtc aaggaaggaa cgtaagaatg aggattgctg ctcgagctga atttgtgtct
    79261 cttcgcgctc tttgatttca attttcagtt ggccgaaggt ttcctgcagt tctgcttccg
    79321 ctttttcacg aacggcgatt tcctgattta gctgggcaat attatctttt aactgaacgt
    79381 tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg caccagtcgt gacagacgtt
    79441 gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc cacggcgtaa
    79501 tcagcaaacc aaagaagata gaacgaataa catcaatgct ttcgacctga ccatgcagca
    79561 ccatggttac cgccatttgt accacaatgg caagaacgac gagggccagc gccagtaaca
    79621 ttgagaagcg caccagacct aacttcatca tcaggtcaac ataatactgc gccagcagac
    79681 gaatttgctt cattagggaa ttccttcacg acaacctgcc ccaataatac ccaattctga
    79741 cagttacgtt ataaattgtg tgagaaatgc ggaattcccc tcaatggcct acgccgttgg
    79801 aggtagccac ggacgtccca gcgccgatcc ctgtacgcca tgttcattca gataacgatc
    79861 cagctcgacc attcccgtcc atcgattttc gcaccacagc ggagcaagca gcgtcgggcg
    79921 acgggcgctg gcggaaatgc ggtggtagat cacttccggt ggcgtatggc gaatcatttc
    79981 tccggcagtg agcgtgtaat cctccagttc aattccgttc aaacgccccg cttcccaggc
    80041 tttcgccata atactgcctt tcacaatatg cagcggatgc agctttatgc cgtccacgcc
    80101 ggtttcaacc actcgctcca gcgtttgcaa acattccgtc tgcccttcgc caggcaggcc
    80161 gacaattaag tgggaacaca ccttcagccc gcgctgacgt gccagctggg ttgtacgctg
    80221 ataacaggca aaatcatggc cgcggttgat gcgatgcagc gttttgtcgt gggcggtttg
    80281 tagccccagc tccagccaca cttcgtaacc ctggtcctta tattcgcaaa gcagatccag
    80341 caccgcatcc ggcacgcagt ccgggcgggt accaacacac aaaccgacaa tattggcctg
    80401 gctcaccgcc tgctgataca tagaacgcag cacctgaact tccgcaaagg tgctggtata
    80461 cgcctgaaag taggccagat agcgtttagc gcggttaact aaattcgcct ggtgcgccag
    80521 ttgctcggca atggaacgat gctgctgcgc ttcatcggca aacgaggcca cattacagaa
    80581 tgtgcagcca ccacgcccga tggtaccgtc acggttaggg cagctaaaac cgccgtgcag
    80641 cgtcagctta tgcacctttt gcccataacg acgggtgaga tcaccaccaa acatattgac
    80701 taatttctgt aactgcataa tctgatagac cgcgccttga aaagaggcca aagcctgcca
    80761 tttttagccc aattcggcga tgacctggat caatcgtccc ggcccgcttt tatcaactgc
    80821 ataatcaatc aaaattaccg aaatttcatg cacaatcaca taaatcactt ttgcttatct
    80881 tgtgtcagat ttttttatct ccttatggat tttaggcaaa aacagtagca tgaaacgtta
    80941 ttaccaatta aggcagtata aaatgctggt tttctcgtca gttcaaggca ggataagggt
    81001 taacacgcct ttatgacagt caggaattgg ctgtttctct aacgacgccc cttttagcct
    81061 taaatagaaa atccacttta atttcagcca attaataaag aattttgcgc taaagcacat
    81121 ttctgtacca ataagcttgc catttgacct gtatcagctt tcccgataag ttggaaatcc
    81181 gctggaagct ttctggatga gcagcctgct catcatattt atgcagtaat tgagatcccc
    81241 tcttcaccgt attaaccgat gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta
    81301 tgacacgcaa accccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt tcggaagtgg
    81361 ggttcccgca gagcctgggg gaggttcacg atatgttgta cgataaatcc cttgagaggg
    81421 ataactgtgg tttcggcctg atcgcccaca tagaaggcga acctagccac aaggtagtgc
    81481 gtactgcaat acacgcactg gcccgcatgc agcaccgtgg cgcgattctc gccgatggta
    81541 aaaccggtga cggttgcggc ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg
    81601 cgcaggagcg cggctggcgt ttagcaaaaa actacgccgt cgggatgctc ttcctgaata
    81661 aagatcctga actcgccgct gccgcacgcc gcatcgttga agaagagctg caacgcgaaa
    81721 cgttgtcgat tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg ggtgaaatcg
    81781 ccctctcctc tctgccacgc attgagcaaa tttttgtgaa cgccccggca ggctggcgtc
    81841 cgcgcgatat ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag
    81901 ccgacaaaga cttctacgtc tgtagcctgt cgaatctggt caatatttat aaaggtctgt
    81961 gtatgccggc ggatctgccg cgcttctacc tggacctcgc ggacctgcgt ctggaatcgg
    82021 ctatctgcct gtttcaccag cgcttttcca ctaacaccgt accgcgctgg ccgctggcgc
    82081 agccgttccg ctacctcgcg cataacggtg aaatcaacac catcaccggt aaccgccaat
    82141 gggcgcgtgc gcgtacctat aaattccaga cgccgctgat ccctgacctg cacgacgccg
    82201 cgccgttcgt caacgaaacc ggctctgact ccagttcgat ggataacatg ctggaactcc
    82261 tgctggcagg cgggatggat atcatccgtg ccatgcgtct gttagtgcca ccagcctggc
    82321 agaacaaccc ggatatggac ccggaactgc gtgccttctt tgactttaac tccatgcata
    82381 tggagccgtg ggatggtccg gcagggatcg tgatgtccga tggccgtttc gccgcctgta
    82441 acctcgaccg taacggtctg cgtccggcac gctacgtcat caccaaagat aaactgatca
    82501 cctgcgcctc tgaagtcggt atctgggatt atcagcctga cgaagtggtc gaaaaaggcc
    82561 gcgtagggcc aggcgaactg atggtgatcg acactcgcag tgggcgcatt ctacactcgg
    82621 cagaaaccga tgatgacctg aaaagccgcc atccctataa agagtggatg gagaaaaacg
    82681 tccgccgtct ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg
    82741 acgacgacac gcttgccagc taccagaaac aatttaacta cagcgcggaa gagctggatt
    82801 cggtgatccg cgtactgggt gaaaacggtc aggaagcggt cggttcgatg ggcgacgata
    82861 ccccattcgc cgtgctctcc agccagccgc gcattattta cgactacttc cgccagcagt
    82921 ttgctcaggt aaccaacccg ccaatcgacc cgctgcgcga agcgcatgtt atgtcgctcg
    82981 ccaccagtat cggtcgtgaa atgaacgtct tctgtgaagc agaaggccag gcgcaccgtt
    83041 taagctttaa atcgccgatt ctgctctatt ccgatttcaa acagctcacg acgatgaaag
    83101 aggatcacta ccgcgcagat acgctggata tcacctttga cgtcactaaa accacgctcg
    83161 aagcgacggt gaaagagctg tgcgacaaag ccgaaaaaat ggtacgtagt ggcaccgtgc
    83221 tgctggtgct ctccgaccgg aatatcgcta aagatcgcct gccggttccg gctccgatgg
    83281 cggttggcgc gatccagacc cgtcttgtcg agcaaagcct gcgttgcgat gccaacatca
    83341 tcgtagaaac cgccagcgcc cgtgatccgc accacttcgc cgtgttgctg ggtttcggcg
    83401 cgacggctat ttatccatac cttgcctatg aaacgctggg ccgcctggta gacacccacg
    83461 cgattgccaa agattatcgt accgtgatgc tcaactatcg taacggcatc aacaaaggct
    83521 tgtacaaaat catgtccaaa atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac
    83581 tgtttgaagc ggtcggtctg cacgatgatg tcgtgggcct gtgcttccag ggggcggtta
    83641 gccgcattgg gggggctagc tttgaagact tccagcagga tctgctgaat ctgtcgaaac
    83701 gagcctggct ggcgcgtaag cctatcagcc agggcggcct gctgaaatac gtgcacggtg
    83761 gcgaatacca cgcctacaat ccggacgtgg tgcgcacgct gcaacaggcg gtacagagcg
    83821 gcgagtatag cgactatcag gaatacgcga agctggttaa tgagcgtccg gcaaccacgc
    83881 tacgcgatct gctggcaatt acgccgggtg aaaacgcggt caacattgct gatgttgaac
    83941 cggcaagcga actgtttaaa cgcttcgata ccgccgcgat gtctatcggc gcgttaagcc
    84001 cggaagccca cgaggcgctg gcggaagcga tgaacagcat cggcggtaac tcaaactccg
    84061 gcgaaggcgg tgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc atcaagcagg
    84121 tggcgtccgg tcgcttcggc gttactccgg cgtacctggt caatgccgac gtcattcaga
    84181 taaaagtcgc ccagggcgcg aagccgggtg aaggaggtca gttaccgggt gataaagtca
    84241 ctccttacat tgccaaactg cgctattcgg tgccgggcgt gacgctgatc tccccgccgc
    84301 cgcaccacga tatctactct attgaggatt tagcgcagct gattttcgac ctcaagcagg
    84361 ttaacccgaa agcgatgatc tccgtgaagc tggtttccga accgggcgta ggcaccatcg
    84421 cgactggcgt ggcaaaagct tatgccgatt taatcaccat cgcaggctat gacggcggca
    84481 ccggcgcaag tccgctttca tcggtgaaat acgcaggctg tccgtgggag ctggggcttg
    84541 ttgaaaccca acaggcgctg gttgccaacg gcttgcgtca caagatccgt ttgcaggtcg
    84601 atggcggcct gaaaacgggt gtcgatatca tcaaagcagc gattcttggc gcggaaagtt
    84661 ttggcttcgg cactggcccg atggtagcgc ttggctgtaa atatctgcga atttgccacc
    84721 tgaacaactg cgccacgggt gtagcaactc aggatgacaa actgcgtaag aatcactatc
    84781 acggcctgcc gttcaaagtg actaattact ttgagtttat cgcccgtgaa acccgcgagc
    84841 tgatggcaca gctgggggta acgcgtctgg tggatctgat tggtcgcacc gacctgctga
    84901 aagagctgga cggtttcacc gccaaacagc agaaactggc actgtcgaag ctgctggaga
    84961 ctgctgagcc gcatgctggt aaagcgctct actgcaccga aaacaacccg ccgtttgata
    85021 acggcctgct gaacgcgcag ttgctgcaac aggcaaaacc gtttgtcgat gaacgccaga
    85081 gcaaaacctt ctggttcgat atccgcaaca ccgaccgttc tgtcggcgca tcgctttcag
    85141 gctatatcgc ccagacgcac ggcgatcagg ggctggctgc cgatcctatc aaagcgtact
    85201 tcaacggcac cgcaggccag agcttcggcg tgtggaacgc gggcggcgtg gaactgtacc
    85261 tgaccggtga tgccaacgac tatgtcggta aaggcatggc gggcggctta atcgccattc
    85321 gtcctccggt tggctcagcc ttccgcagcc atgaagcgag cattatcggc aacacctgcc
    85381 tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg
    85441 tgcgtaactc cggtgctatc accgtggtag aaggcattgg cgacaacggc tgtgaatata
    85501 tgacgggtgg tatcgtctgt attctgggta aaaccggcgt taacttcggc gcaggcatga
    85561 ctggcggctt cgcttacgtt ctcgatgaaa gcggcgattt ccgcaaacgc gttaacccgg
    85621 aactggtcga ggtcttaagc gttgacgatc tggcgatcca tgaagagcat ctgcgcggtc
    85681 ttatcaccga gcatgttcag cataccggtt ctcagcgcgg tgaagagatt ctggcgaact
    85741 ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac
    85801 tgctgggtca ccgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag taaggggtag
    85861 caacaatgag tcagaatgtt tatcaattta tcgacctgca gcgcgttgat ccgcctaaga
    85921 aaccgctgaa gatccgcaaa attgagtttg ttgaaattta cgagccgttt tccgaaggcc
    85981 aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa cccatactgc gagtggaaat
    86041 gcccggtaca caactacatc ccgaactggc tgaagctcgc caacgagggg cgtatttttg
    86101 aagcggcgga actgtcgcac cagaccaaca ccctgccgga agtatgcgga cgtgtctgcc
    86161 cgcaagaccg tctgtgcgaa ggttcctgca ccctgaacga tgagtttggc gctgtgacca
    86221 tcggcaacat tgagcgttat atcaacgata aagcgttcga aatgggctgg cgtccggatc
    86281 tgtccggcgt gaaacagacc ggtaaaaaag tggcgattat cggcgctggc ccggcgggtc
    86341 tggcgtgtgc ggatgttctg acgcgcaacg gcgtaaaagc ggtcgtcttc gaccgtcacc
    86401 cggaaattgg cggattgctg accttcggta ttccggcttt caagctggaa aaagaggtaa
    86461 tgacgcgccg ccgtgaaatc ttcaccggca tgggtattga attcaaactc aataccgaag
    86521 tgggccgcga cgtgcagctg gacgatctgc tgagtgatta cgatgccgtg ttccttggcg
    86581 tcgggactta tcagtcaatg cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg
    86641 cagcgctgcc gttccttatc gccaacacca aacagttaat gggctttggt gaaacccgcg
    86701 acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt ccttggcggt ggcgacactg
    86761 cgatggactg cgtgcgcacg tctgtacgcc agggggcaaa gcacgttacc tgtgcctatc
    86821 gtcgtgatga agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag
    86881 gcgtagagtt caaattcaac gtccagccgc tgggtataga agtgaatggc aacggcaaag
    86941 tcagcggcgt aaaaatggtg cgcacagaaa tgggcgaacc ggatgccaaa ggccgtcgcc
    87001 gcgcggagat tgtggcaggt tccgaacata tcgttccggc agatgcggtg atcatggcgt
    87061 ttggtttccg tccacacaac atggaatggc tggcaaaaca cagcgtcgag ctggattcgc
    87121 aggggcgcat tatcgccccg gaagacaacg acaacgcttt ccagaccagc aacccgaaaa
    87181 tctttgctgg cggcgatatc gtccgtggtt ccgatctggt ggtgaccgct attgccgaag
    87241 gtcgtaaggc ggcagacggt attatgaact ggctggaagt ttaagcctgt cccagcgccg
    87301 cctcctcaga aaagagtcgg cgctgaaatt ctcctttctt tctggaaagc gcctcgcata
    87361 atctgaagaa aaccaatgta atacgctcat ctggattgtt accggtagat gcccggcgta
    87421 tagctgattc cattattcaa cgcagcgcca tataaaggga cagataatgg aatcgctctc
    87481 agagagaacc tcaacaggct accagcaaat ccacgacggt attattcacc tggtcgatag
    87541 cgcccggacg gaaacggtac gtagcgttaa cgcgctgatg accgcgacat actgggaaat
    87601 tggccgacga attgtcgaat ttgaacaagg tggcgaggcc agggctgcgt atggtgcgca
    87661 gctaatcaag cgactatcaa aggatttaag tctaaggtat aagcgtgggt tctctgcaaa
    87721 aaacttacgc caaatgaggc ttttttacct cttttttcaa catgttgaaa ttcgccagac
    87781 agtgtctggc gaattaacac cattgggaat tccgcagaca ccatctgcag aattcccatc
    87841 ggcaaagatt tggcagacgc tgtctgccaa atctttcccc ctgccctggt ccacctacgt
    87901 ccgtttactt tccgttaaaa accctgatgc ccgcagcttt tatgaaaaag agacgctccg
    87961 ctgtggttgg tctgtccgcc agctagagcg gcaaattacg acccaatttt atgagcgaac
    88021 actactgtca catgacaaat cagccatgct gcaacaacac gctcctgccg agatgcatat
    88081 tcttccgcaa caggcgatac gcgatccctt tgtgctcgaa tttctggagt tgaaagatga
    88141 atactcagaa tctgattttg aggaggcgct gatcaaccac ctgatggatt tcatgctgga
    88201 acttggggat gattttgcct ttgttggtcg gcagcgaagg ttacgcattg atgacaactg
    88261 gtttcgggtc gatctgctgt ttttccaccg ccgtttacgc tgcctgctaa tcgtcgatct
    88321 aaaagtgggc aaattcagct atagcgatgc cggacagatg aatatgtatc tcaactacgc
    88381 caaagagcac tggacactac cggatgaaaa tccgcccatc ggtctggttc tctgtgcaga
    88441 gaaaggagcc ggcgaagcgc attatgcact ggctggtttg cctaacaccg ttctggcgag
    88501 tgaatataag atgcaactac ctgatgaaaa acgactcgcg gatgaactcg ttcgaacaca
    88561 ggcggtgtta gaggaaggct atagacgccg ttaatttcag cggtttcgaa ttctccagac
    88621 actgtctgga gaatctttaa aacgaactaa gttttaagcc attaaatcag ccttaacctt
    88681 caccacaacc tttttaatct caccaggttc gccgacaacg catcctggtt tatgcggttc
    88741 tcccggcata aacacggcga acattcccgg ttttaagatg atggcttgct cgttctcaat
    88801 ggcgctgcaa agctggtaat catcctcatg gtggaactct tcacactgac gcgcagtgcc
    88861 tgccgtgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
    88921 ctcgtgcagc tccgcttttt tctcaacggg cgattgagtg ttaaacgtca tgacattcat
    88981 aaagatattg tcgcactgta attcgtaacg acccggtgct ttttcttgcg gcctggcggc
    89041 taaggctagt gttaacgcgt cctgtaacgc aggatgcaat ccggcagacg gtaacgactg
    89101 tacttcaccc attatcataa tttttctccc tgggccaaca acgcagcccc aagtaaacct
    89161 gcatcgtggc ggtagtgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
    89221 agatacgttt ccaccaacgc cagataccct tctgccagac caacgctgcc accgaccact
    89281 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgccag cacatgtgcg
    89341 gagcagtgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
    89401 ctcgcatccg cgcccgccag ttcaccctgc gctgccgctg caataccgcg accagaagca
    89461 atcgcttcca cacaaccagt gcgtccacag ccgcagactg gaccatgagg atcagccagc
    89521 gtatgcccga tgtgccccgc cagaccgcca gggccggtga gcagtttgcc gccgctcact
    89581 acaccgccgc caacaccagt ggaaacggtg ataaacacca tctcggttat atcgccttcc
    89641 agcgcctgat actccgccca cgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
    89701 tcggtaagtt gttccaaagt tttgactaac ggaaagtgta gcaatccacc gagattatgc
    89761 ggattcagcg ccagcaggct gccgtcgcga ataattccgg ttgaagcgat agcaacccgc
    89821 tgcgcatgaa cttgcaacgg agagactaat gcggataagg catcatgcaa ggcttctggt
    89881 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccatc agcgccaatc
    89941 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtgtggtcat agcaccgcct
    90001 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
    90061 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
    90121 gcccttcggc aatcacccga catccggcgt cgctcaacgt tttcaccagc gccagatccg
    90181 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
    90241 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
    90301 cgtgatggtg aatacgtgcc agcagcgttt caacaggcac cggacgcggt cggtcggtgc
    90361 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcaacatct tcaatatagg
    90421 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aatcccgata attggcacgc
    90481 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc aatgcgaatg gcaaccgcgc
    90541 ccgcctgttc tgccgctaat gccatggcgg cgacgatttc gggtttatcg agcgggctgt
    90601 ccggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
    90661 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacagaga ccgggcaaca
    90721 ggattaactt ttggttttga ctaaatcgtt tttggcgcgg ccaaatggca cggcaccgct
    90781 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
    90841 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca gactgaacga
    90901 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
    90961 ggccagtgca ccgcccaatg cgccgacgtt gtaggtaaag cccaggcccg ctgcacgctg
    91021 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
    91081 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
    91141 taccggaata atcagcagct gcgaggccag cagactacaa acgtacgctt tgcgggttcc
    91201 cagccagtca ccgaggaagc caccgacgca gcaccccacc gccgcgccaa agccactaaa
    91261 gaacagcaca ttggcgacag tatgcgggtc ataagccaga tcggttttca gatacgttgg
    91321 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
    91381 tacgcccgtt ggccagcgtt tgcccgtact ctgcaccata aagctgataa agattgcggc
    91441 gcataacagc ccgagtacag cgacgattgc ggcattttgc aggttaccgg cgaagcagaa
    91501 ccacagcgca gtagccgccg ccagcgtcat tacgatattg gcaatgcgat gttcaccacg
    91561 gtaaagaatg tctaccatcg tgcgtactgg tgctttaccc ccgtgtttct ctttccagtc
    91621 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
    91681 aaagaacagc gctctccagc cccagaccgg aaccaccaga ctatagacct gagcggcaac
    91741 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
    91801 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
    91861 gatgaccaga cgcgcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
    91921 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
    91981 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
    92041 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
    92101 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
    92161 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
    92221 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
    92281 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
    92341 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
    92401 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
    92461 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
    92521 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
    92581 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
    92641 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
    92701 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
    92761 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
    92821 gcaaaccatc cgccgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
    92881 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
    92941 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
    93001 tacctttcgc ctcttcagcg acgatttcca gtacctgttc acgctcggaa aggctttgta
    93061 caaaggcttc gccggtagaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
    93121 actgaaccag gcgacgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
    93181 ggagtgcggc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgctta
    93241 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
    93301 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
    93361 tgaccgaagg cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
    93421 gcttcatcgg gatcgtggcg gcggatcgcg tcaacgatcg caatatgctg ttgatagcta
    93481 acgttgttat gctcgtgcag agcctgatca gccacggttg gacgtgcggc gatcagccag
    93541 tcgagcaagg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
    93601 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
    93661 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
    93721 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccgccagga
    93781 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatggtgtc cgcagaagga
    93841 cgtgagacgc gagcgcgttc gccgttgttt atctgcacca gacctttgcg ttttaacgct
    93901 gccagcgctt cgcgcaccga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
    93961 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
    94021 tccaccattt cggagagttt tttacgcgcc agcgggcggc tgcgtaagtt gcggccaatt
    94081 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
    94141 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg gggaaatggc
    94201 ggggttttct ctttgtgcct catcattact ataattaact gaataattaa ctattgcgaa
    94261 aaattaatgt aacgcagata aaaacatccc gattgaatta ttcataagac tattaacaag
    94321 tattatgaat attatgaatg tgttcttaca aaataatcat aagcgcatat tttttaatga
    94381 aaaatcacct catctacaat taaaaacacg acatccgcac cataaatagc cttgcaaaaa
    94441 atataacatc attgttttca atctgccgtt tatgggattg accattttct tttgacacgg
    94501 agttcaacaa tgttcggcat aattatatct gtcatcgtat taattacgat gggctatttg
    94561 atcctgaaaa actacaaacc tcaggtggtg ctggctgccg caggtatctt cctgatgatg
    94621 tgcggtgtct ggttagggtt cggtggtgtg ctcgatcccg ccaaaagcag cggctacttg
    94681 atcgtcgata tttataatga aatcctgcgc atgctgtcca accgcattgc tggattgggg
    94741 ctgtcgatta tggcagtggg cggttatgcc cgctatatgg agcgcatagg ggcaagtcgc
    94801 gcgatggtga gcttgttaag ccgcccgtta aaactcattc gctcgccgta tattattctg
    94861 tcggcaactt acgtcatcgg ccaaatcatg gcgcagttta ttaccagcgc ctccggtctg
    94921 ggtatgttgc tgatggtcac cttatttccg acgctggtga gtctgggagt aagtcgtctc
    94981 tctgcggtgg cggttatcgc aaccaccatg tccattgagt gggggattct ggaaacgaac
    95041 tccatttttg ctgcccaggt cgcaggaatg aaaattgcca cttacttctt ccactaccag
    95101 cttccggtcg cctcttgcat cattatctcg gtggcgatct cccacttttt cgtgcaacgc
    95161 gctttcgaca aaaaagataa aaatatcaat cacgaacagg cagagcaaaa agctctcgat
    95221 aatgtcccgc cgctctatta cgccatttta ccggtgatgc cgttaatcct gatgctcggc
    95281 tcgctgttcc ttgcccacgt cgggctgatg cagtcagaac tgcatctggt ggtggtgatg
    95341 ttactgagtt tgacggtgac gctgtttgtt gagttcttcc gcaagcataa cttgcgcgaa
    95401 acaatggacg atgtgcaggc gttttttgac ggcatgggta cgcagtttgc caacgtggta
    95461 acgctggtgg tcgcgggtga aatatttgcg aaaggcttaa cgacgattgg cactgtcgat
    95521 gcggttatca ggggggcgga gcattctggt ctgggcggta ttggcgtgat gattattatg
    95581 gcgctggtca ttgccattgg tgccattgtg atgggctcag gcaatgcgcc gtttatgtca
    95641 tttgccagtc ttattccgaa tatcgcagcc ggactacatg taccagcggt tgtaatgatt
    95701 atgccgatgc attttgccac gacgctagcg cgcgctgttt cgccgattac tgcggtggtg
    95761 gtcgttacgt caggaattgc aggcgtttcg ccttttgcgg tagtgaagcg gacagcgatc
    95821 cccatggcag tcggtttcgt ggtgaatatg attgccacta tcacgctatt ttattaagcg
    95881 attaaaaaga caaaacaggc cgccagggcc tgttttcatt ttacttcaca acgcgtaatg
    95941 ccggtcgacc accgcgtggt ggctgcggag gttcatcgtc aggatgagtg tcatcatcgt
    96001 gatctggctt gtcgccatca ataaccgaca taacggtttc gttgtctgcc gatgcctctt
    96061 catcattcat gatgctggca tcttcatcgt aggcagcttc aggttcaaac atcgtgcctg
    96121 cgccattttc acgggcgtag atagccagca cggcagccag cggcacagaa acctgacgcg
    96181 gaatgccacc aaagcgcgcg ttaaagcgca cctcatcatt cgccagttcc agattgccga
    96241 cagctcgcgg cgcaatgttg agtacgattt gcccgtcacg cgcatattcc ataggaacct
    96301 gcacgccagg gagcgtcaca tccaccacca ggtgcggcgt gagctggtta tccagcaacc
    96361 actcatagaa tgcacgcagc agatagggac gacgtggtgt tagctgtgac aaatccatac
    96421 agattaactc cggcccagac gcatttcacg ttcagcttca gttaaagaag caaggaaaga
    96481 gtcacgctca aagacgcggg tcatatagcc tttcagctct ttcgcacccg ggccgctgaa
    96541 ctcgatgccc agttgcggca gacgccacag cagaggagca aggtagcaat cgaccaggct
    96601 gaactcatcg ctcaggaagt acggcttctg accgaagacc ggcgcaatcg ccagcagttc
    96661 ttcgcgcagt tgcttacgtg cggcatcggc ttcagaagct gaaccgttga tgatggtgtt
    96721 catcagcgtg taccagtctt tttcgatgcg atgcatgtac agacggcttt caccgcgagc
    96781 taccgggtaa acaggcatca gtggcggatg cgggaaacgc tcatccagat attccataat
    96841 gatgcgagat tcccacaggg tcagctcacg atccaccagg gtcggaacgc tctgattcgg
    96901 gttgaggtca atcagatcct gaggcggatt gtccttttcc acgtgttcga tctcgaaact
    96961 tacacctttc tcagccagca caatgcggac ctgatggcta tagatgtcag taggaccgga
    97021 aaacagcgtc attaccgaac gtttgttggc agcgacagcc atgaaaacct ccaggtatag
    97081 tcagaatttt tactgctacc agccaccagg tggccagtca gaagttgtgt tacccagtaa
    97141 ggaacgactc tctttgttcg aaaatcaaac aaaaaatgag caatacccga catttgggca
    97201 gaaaattgga tgatagttta ccagattttg cgacctttgt ggtgagtcga tgccggaaat
    97261 ggggaaaaag agatgcgctt tagtctgaaa tagttgactt agtcccttat tggcgatgtg
    97321 gtttttgttt tacctgtctg tcaggtggca gcaaaaagca acttcccatt ttttacgctg
    97381 atgcagattt tagccataaa aaaacccgcc gaagcgggtt ttttcgcaaa ttgttttctg
    97441 ccggagcaga agccaattaa cgtttggaga actgcggacg acgacgtgct ttacgcagac
    97501 cgactttctt acgttcaacc tgacgagcgt cacgagtaac gaagccagct ttacgcagtt
    97561 cagaacgcag ggactcgtcg tattccatca gagcgcgggt gataccgtga cggatcgcac
    97621 cagcctgacc agagatacca ccacctttaa cggtgatgta caggtccagt ttctcaacca
    97681 tgtcgaccag ttccagcggc tgacgaacta ccatgcgagc agtttcacga ccaaagtact
    97741 gttccagaga acgttggttg attacgattt taccgttgcc cggtttgatg aaaacgcgag
    97801 ctgcggaact tttgcggcga ccagtgccgt agtattgatt ttcagccatt gcctataatc
    97861 ccgattagat gattagatgt caagaacttg cggttgctgt gccgcgtggt tgtgctcgtt
    97921 acccgcgtaa actttcagtt tacggaacat agcacgaccc agcgggcctt ttggcaacat
    97981 gcctttaacc gcgatttcaa tcacacgctc aggacggcga gcaatcatct cttcaaaggt
    98041 cgcttgtttg ataccaccga tgtggccggt gtggtgatag tacactttgt cagtacgctt
    98101 gttgccggtt acagcaactt tgtcagcgtt cagaacgatg atgtaatcac cggtatctac
    98161 gtgcggagtg tattccgctt tgtgcttacc gcgcaggcga cgagccagtt cagtagccag
    98221 acggcccaga gttttaccgg tcgcgtcaac aacataccag tcgcgtttta cggtttctgg
    98281 tttagctgta aaagttttca ttaaaagctt acccaataaa aagttacacg ttggtgaaca
    98341 cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc cggcaaacct accccttcga
    98401 atagcctatg ccagcacaca aaaagttttg ggaaaaaaac tttcttgtaa cgtggggtcg
    98461 caggattata gagaagtcgg ggtcaaagat cgaccccttt ttgtgatttg tggcaggttt
    98521 taacccgcca aatgctcgcg cttcagatac tcttcgcttt gcatctcttg cagacgtgac
    98581 aggcaacgct ggaactcaaa tttcagccgc tcgccctgat aaatgtcata tagcggcact
    98641 tctgcactca ccactaattt gacatggcgc tcgtaaaact catccaccag cgcaataaaa
    98701 cgtcgcgctt cgctctccat caaccgcgtc ataactggta catcaaacaa catgactgtg
    98761 tgaaagaggc gtgagagcgc aatatagtca tgctgactgc gggcgtcgac gcacagcgta
    98821 gtaaaagaga ccgccagcgt ctggttctcg acgcccattg tcgccagcgg ccgatggttg
    98881 atttctaatg tcggtgaatt ttctcgtttc gcccccgcca gcgccaacca tagtttatcc
    98941 atttgcgccc gggtttcatc gttgaggggc gaaagccaca gatgcgcctg agtgagtgta
    99001 cgcagtcgat aatcaacacc agcgtccacg ttcattacat cacaatgctg tttaatggca
    99061 tcgattgcag gcaaaaaacg cgcacgttgt aggccatttc gataaagttc gtccggcgga
    99121 atatttgacg tcgctaccag ggtaataccg cgggcgaaca gggctttcat cagaccgcca
    99181 agtagcatgg catcggtaat atcagaaaca aaaaattcgt caaaacagag cacgtcagtt
    99241 tctgctttaa agcgatcggc aataatttcc agcggatcgg tctgcccctg caaagcagtt
    99301 agctcttcat gtacccgcag cataaaacgg tgaaagtgca gacgctgttt ccgctctccc
    99361 ggcaggcttt gatagaaaag gtccatcagc caggttttcc cgcgtcctac accgccccac
    99421 atatataagc cacgcactgg cgtatgcttt gtgtcttcgc gtttacccca cagcttaccg
    99481 acccgcgcca ttagcccact cgtcctgggg gctggaggcg tgctattgat gagttcctga
    99541 taaataattt ccaggcggct gacggcctct ttttgaacgt cgtcgggttg atggctgcct
    99601 tcattgagcg ccttcaggta ttgcgatgtt ggggtaacgc tttgcatgat cttattgtta
    99661 ttccttgaat aatcggtgcg ccgttgttca cggttgacga aaaaaaggcc gttctacact
    99721 acgcgatatg cagtcgggat tccacttctg tggaattaac ggttatagtg gcataatcag
    99781 ccgcaggcat ggagcctgaa gccaacaccc tacggaaaca aaagacaacg ggagatgttc
    99841 atgacctggg aatatgcgct aattgggtta gtcgtcggca tcattattgg tgctgtggcc
    99901 atgcgttttg gtaatcgtaa actacgccag caacaggcgt tgcagtacga actggaaaag
    99961 aataaagctg aactggacga gtatcgcgaa gagctggtta gccactttgc ccgcagcgcg
   100021 gaattactgg ataccatggc gcacgactat cgccagctgt atcaacacat ggcgaaaagc
   100081 tccagcagcc tgctgccgga actgtctgct gaagcaaacc cgttccgtaa tcgtctggca
   100141 gagtctgaag ccagcaacga tcaggcaccg gtgcaaatgc ctcgcgacta ttctgaaggc
   100201 gcatccggcc tactgcgtac tggcgcgaag cgcgactaat ttatttttcg ggcgcagcca
   100261 ttgcgccctc ctcttctctc cctccccgac tatcatttaa tctggtgtct cattgttagc
   100321 cgtctgaaaa ttcaataaca tcaaactgtt ttgaatctct tttcttatca ttcaggtacg
   100381 agagtaggaa taatgaaaaa acaaacccag ctgttgagtg cattagcgtt aagtgtcggg
   100441 ttaactctct cggcgtcatt tcaggccgtt gcgtcgattc caggccaggt tgccgatcag
   100501 gcccctctcc ccagtctggc cccaatgctg gaaaaagtgc ttccggcagt ggtgagcgta
   100561 cgggtggaag gaacggccag tcagggacag aaaatcccgg aagaattcaa aaagtttttt
   100621 ggtgatgatt taccggatca accggcacaa cccttcgaag gtttaggctc cggtgtcatc
   100681 atcaacgcca gtaaaggcta tgtgctgact aacaaccatg tgattaatca ggcacagaaa
   100741 atcagtattc agctcaatga tgggcgcgag tttgatgcaa aactgattgg tagcgatgac
   100801 cagagcgata tcgccctgtt gcaaattcaa aacccgagca aattaacgca aatcgctatt
   100861 gccgactccg ataaattgcg cgtcggtgat tttgccgtgg cggtcggtaa cccatttggc
   100921 cttgggcaaa ccgccacctc tggcattgtt tccgcattag gccgcagtgg gttgaatctt
   100981 gaaggtctgg aaaactttat ccagacagat gcttccatta accgcggtaa ctccggcggt
   101041 gcactgttaa atcttaacgg tgagttaatt ggcatcaaca cggcaatcct tgcgcctggt
   101101 ggcgggagcg tcgggattgg atttgccatc cccagtaata tggcgcgaac actggcgcag
   101161 cagcttatcg actttggtga aatcaaacgc ggtctgttag gcatcaaagg cacagagatg
   101221 agtgccgata tcgccaaagc cttcaacctt gacgtgcagc gtggcgcgtt tgtcagcgaa
   101281 gtgttgccag gttctggctc ggcaaaagcg ggcgtcaaag cgggcgatat tattaccagc
   101341 ctcaacggca aaccgctgaa tagctttgct gagttgcgct ctcgtatcgc aaccaccgag
   101401 ccgggcacga aagtgaagct tggcctgctg cgtaacggca aaccactgga agtagaagtg
   101461 acgctcgata ccagcacctc ttcgtcggcc agcgctgaaa tgatcacgcc agcgcttgaa
   101521 ggtgcaacgt tgagcgatgg tcagctaaaa gatggcggca aaggtattaa gatcgatgag
   101581 gttgtcaaag gaagcccagc tgctcaggca ggcttgcaaa aagacgatgt gatcattggc
   101641 gtcaaccgcg atcgggtgaa ctcgattgct gaaatgcgta aagtgctggc ggcaaaaccg
   101701 gccatcatcg ccctgcaaat tgtacgcggc aatgaaagca tctatctgct gatgcgttaa
   101761 tgtcgtaaac cgggcatcag gctgacgtgt gatgtccggt taactcgtgg tatgctgctg
   101821 ccgttccctt ttttaatgac gcctccatca tgtttgtgaa gctcttacgt tccgttgcga
   101881 ttggattaat tgtcggcgct attctgctgg ttgccatgcc ttcgctgcgc agccttaacc
   101941 cgctttccac tccgcaattt gacagtaccg atgagacgcc tgccagctat aatctggcgg
   102001 ttcgccgcgc cgcgccagcg gtggttaacg tttacaaccg tggtttgaac accaactctc
   102061 acaaccagct tgagatccgc accctgggat ccggtgtaat catggatcaa cgcggttata
   102121 tcatcaccaa taaacacgtc atcaacgacg ccgatcagat catcgtcgcc ttacaggatg
   102181 gacgtgtttt tgaagcgtta ctggttggct ctgactctct aaccgatctg gcggtgctga
   102241 aaattaacgc cacaggcggt ttaccaacga ttccgatcaa tccacgccgc gtaccgcaca
   102301 ttggcgatgt ggttctggcg atcggtaacc cgtacaacct cgggcagacc attacccagg
   102361 ggattatcag tgccacgggt cgaatcggtc tgaacccgac cgggcggcaa aacttcctgc
   102421 aaaccgatgc ttccattaac cacggtaact ctggcggcgc gctggtgaac tcgctgggcg
   102481 aactgatggg cattaacacg ctgtcgtttg ataagagtaa cgatggcgaa acgccggaag
   102541 gtatcggctt tgcgattcct ttccagttag cgaccaaaat tatggataaa ctgatccgtg
   102601 atggccgtgt gatccgtggc tacattggca ttggtggacg tgagatcgcc ccattacatg
   102661 cgcagagtgg cggcatggat caactgcaag ggatcgtggt taacgaagtg tcgcctgatg
   102721 gcccagcggc aaatgcaggc atccaggtca acgatctgat tatctctgtg aataacaaac
   102781 ccgctatttc cgccctggag acaatggatc aggtcgcgga aattcgccct ggttcggtga
   102841 tcccggttgt cgtgatgcgt gacgataaac agttaacatt gcaggtcacc attcaggaat
   102901 atccggcgac caactaaggt cagcgtaaaa aaacaaaaac cggagtctgt gctccggttt
   102961 tttattatcc gctaatcaat tacttattaa cgaactcttc gcccagggcg atatctttct
   103021 tcagcgtatc cagcatacct tccagcgcgt tctgttcaaa tgcgctcagg gtaccgatag
   103081 atttacgctc ttccacgccg tttttaccca gcagcagcgg ttgagagaag aaacgtgcgt
   103141 actgaccgtc gccttcaacg taggcacatt cgacaacgcc ttgttcgccc tgcagtgcgc
   103201 gaaccagaga cagaccaaaa cgtgcagctg cctggcccat agacagggtt gcagacccgc
   103261 caccggcttt cgcttcaacc acctcagtac ccgcgttctg gatacgtttg gtcagatcag
   103321 ccacttcctg ctcggtaaaa ctaacgccag gaacctgtga cagcagcggc agaatggtaa
   103381 caccagagtg accgccaata accggcactt caacttcgcc tggctgtttg cctttcagtt
   103441 ccgcaacaaa ggtgttggaa cgaatgatat ccagcgtggt aacgccgaac agtttgtttt
   103501 tgtcataaac accggctttt ttcagcactt cagcagcaat cgcaactgtg gtgttaaccg
   103561 ggttagtgat aataccaatg cacgctttcg ggcaggtttt cgcaacttgc tgtaccaggt
   103621 ttttcacgat gccggcgtta acgttaaaca ggtcggaacg atccataccc ggtttacgcg
   103681 ctacacctgc agagataaga acgacatctg cgccttccag cgccggagtc gcatcttcac
   103741 cagaaaaacc tttgattttc acagcagtag ggatatggct cagatcgaca gccacaccgg
   103801 gagtcactgg agcgatatca tacagagaga gttctgaacc tgaaggcagt tgggttttta
   103861 acagtagtgc aagcgcctgg ccaataccgc cagcagcgcc gaggactgcg actttcatcc
   103921 taaactcctt attatattga taaactaaga tatgttgctc cgctgccgcg accttaatcc
   103981 acaaaattgc cgtttacaat gaccacatct caagaatgtg tagtcacgca agtttagcgt
   104041 ttatgcattt aattgccgta atcaggaacc taacttacgt aattaacagt cgcttatgca
   104101 ttagcgcaac attccaacag gtggtgacaa tataccctac cgttcagcca aaacaacatc
   104161 aatttgataa caattaattt acttttaagc agaatttgca tgccgtgacg caggcatgtt
   104221 tctcaataac gaaatttgat aaaatcccgc tctttcataa cattatttca gccttcttca
   104281 gggctgactg tttgcataaa aattcatctg tatgcacaat aatgttgtat caaccaccat
   104341 atcgggtgac ttatgcgaag ctcggctaag caagaagaac tagttaaagc atttaaagca
   104401 ttacttaaag aagagaaatt tagctcccag ggcgaaatcg tcgccgcatt gcaggagcaa
   104461 ggctttgaca atattaatca gtctaaagtc tcgcggatgt tgaccaagtt tggtgctgta
   104521 cgtacacgca atgccaaaat ggaaatggtt tactgcctgc cagctgaact gggtgtacca
   104581 accacctcca gtccattgaa gaatctggtg ctggatatcg actacaacga tgcagttgtc
   104641 gtgattcata ccagccctgg cgcggcgcag ttaattgctc gcctgctgga ctcactgggc
   104701 aaagcagaag gtattctggg caccatcgct ggcgatgaca ccatctttac tacccctgct
   104761 aacggtttca ccgtcaaaga cctgtacgaa gcgattttag agctgttcga ccaggagctt
   104821 taatctctgc cccgtcgctt ctggcggcgg ggaaaatgtt gcttttccct ctcccccccc
   104881 ctgctttctc ctgcgattaa tttaacgaat agtgcgtttt atcgcga
//
LOCUS       DAZGHF010000003        96101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGHF010000003 DAZGHF010000000
VERSION     DAZGHF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.003"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(111..596)
                     /gene="sixA"
                     /locus_tag="ACHK3A_000215"
     CDS             complement(111..596)
                     /gene="sixA"
                     /locus_tag="ACHK3A_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO8738368.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(799..2943)
                     /gene="fadJ"
                     /locus_tag="ACHK3A_000216"
     CDS             complement(799..2943)
                     /gene="fadJ"
                     /locus_tag="ACHK3A_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO8738369.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSGGYDAEARAFGEL
                     VMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDTALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            complement(2943..4253)
                     /gene="fadI"
                     /locus_tag="ACHK3A_000217"
     CDS             complement(2943..4253)
                     /gene="fadI"
                     /locus_tag="ACHK3A_000217"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO8738370.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLLDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEMDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(4434..4718)
                     /gene="yfcZ"
                     /locus_tag="ACHK3A_000218"
     CDS             complement(4434..4718)
                     /gene="yfcZ"
                     /locus_tag="ACHK3A_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO8738371.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5090..6430
                     /gene="fadL"
                     /locus_tag="ACHK3A_000219"
     CDS             5090..6430
                     /gene="fadL"
                     /locus_tag="ACHK3A_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO8738372.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIRQSPAWLTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNPAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            complement(6492..7247)
                     /gene="mlaA"
                     /locus_tag="ACHK3A_000220"
     CDS             complement(6492..7247)
                     /gene="mlaA"
                     /locus_tag="ACHK3A_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO8738373.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            7541..8473
                     /gene="yfdC"
                     /locus_tag="ACHK3A_000221"
     CDS             7541..8473
                     /gene="yfdC"
                     /locus_tag="ACHK3A_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO8738374.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKTIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            8549..8623
                     /locus_tag="ACHK3A_000222"
     tRNA            8549..8623
                     /locus_tag="ACHK3A_000222"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8582..8584,aa:Arg,seq:cct)
     gene            8803..8985
                     /locus_tag="ACHK3A_000223"
     CDS             8803..8985
                     /locus_tag="ACHK3A_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8738375.1"
                     /translation="MWRDSILHSAMHSLMNSQARDTRDTSLNDDNVLEISEPRIRSTF
                     ERLYQNMNEAFGLGDY"
     gene            complement(9065..10312)
                     /locus_tag="ACHK3A_000224"
     CDS             complement(9065..10312)
                     /locus_tag="ACHK3A_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8738376.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(10384..11298)
                     /locus_tag="ACHK3A_000225"
     CDS             complement(10384..11298)
                     /locus_tag="ACHK3A_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO8738377.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDWDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            11514..12947
                     /gene="cscA"
                     /locus_tag="ACHK3A_000226"
     CDS             11514..12947
                     /gene="cscA"
                     /locus_tag="ACHK3A_000226"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO8738378.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDSGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(12955..13950)
                     /locus_tag="ACHK3A_000227"
     CDS             complement(12955..13950)
                     /locus_tag="ACHK3A_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8738379.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHISLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEDREGGRVTRIPCPLLIRCST"
     gene            14193..14411
                     /locus_tag="ACHK3A_000228"
                     /pseudo
     CDS             14193..14411
                     /locus_tag="ACHK3A_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            14429..15760
                     /gene="dsdA"
                     /locus_tag="ACHK3A_000229"
     CDS             14429..15760
                     /gene="dsdA"
                     /locus_tag="ACHK3A_000229"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO8738380.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLTQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLHNATHLVWATGGGMVPEEEMEQYLAKGRS"
     gene            complement(15865..17403)
                     /gene="emrY"
                     /locus_tag="ACHK3A_000230"
     CDS             complement(15865..17403)
                     /gene="emrY"
                     /locus_tag="ACHK3A_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO8738381.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(17403..18566)
                     /gene="emrK"
                     /locus_tag="ACHK3A_000231"
     CDS             complement(17403..18566)
                     /gene="emrK"
                     /locus_tag="ACHK3A_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO8738382.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTTPIEKEISNIISHNGQL"
     gene            18982..19596
                     /gene="evgA"
                     /locus_tag="ACHK3A_000232"
     CDS             18982..19596
                     /gene="evgA"
                     /locus_tag="ACHK3A_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO8738383.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            19601..23194
                     /gene="evgS"
                     /locus_tag="ACHK3A_000233"
     CDS             19601..23194
                     /gene="evgS"
                     /locus_tag="ACHK3A_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO8738384.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(23250..24395)
                     /gene="yfdE"
                     /locus_tag="ACHK3A_000234"
     CDS             complement(23250..24395)
                     /gene="yfdE"
                     /locus_tag="ACHK3A_000234"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO8738385.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(24469..25413)
                     /gene="yfdV"
                     /locus_tag="ACHK3A_000235"
     CDS             complement(24469..25413)
                     /gene="yfdV"
                     /locus_tag="ACHK3A_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO8738386.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(25483..27177)
                     /gene="oxc"
                     /locus_tag="ACHK3A_000236"
     CDS             complement(25483..27177)
                     /gene="oxc"
                     /locus_tag="ACHK3A_000236"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO8738387.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNGIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(27231..28481)
                     /gene="frc"
                     /locus_tag="ACHK3A_000237"
     CDS             complement(27231..28481)
                     /gene="frc"
                     /locus_tag="ACHK3A_000237"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO8738388.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(28994..29626)
                     /gene="yfdX"
                     /locus_tag="ACHK3A_000238"
     CDS             complement(28994..29626)
                     /gene="yfdX"
                     /locus_tag="ACHK3A_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO8738389.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            29922..30197
                     /gene="ypdI"
                     /locus_tag="ACHK3A_000239"
     CDS             29922..30197
                     /gene="ypdI"
                     /locus_tag="ACHK3A_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO8738390.1"
                     /translation="MKVNLMIFSLFLLVSIMACNVFAFSISGGRSETSYKETEKTSVM
                     TTTHSTKLQPSQAILFKLREDAPPLNLTEDITPPFSTKANYLIHPVR"
     gene            complement(30274..30516)
                     /gene="yfdY"
                     /locus_tag="ACHK3A_000240"
     CDS             complement(30274..30516)
                     /gene="yfdY"
                     /locus_tag="ACHK3A_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO8738391.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            30869..31789
                     /gene="lpxP"
                     /locus_tag="ACHK3A_000241"
     CDS             30869..31789
                     /gene="lpxP"
                     /locus_tag="ACHK3A_000241"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO8738392.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            32145..32216
                     /gene="ypdK"
                     /locus_tag="ACHK3A_000242"
     CDS             32145..32216
                     /gene="ypdK"
                     /locus_tag="ACHK3A_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO8738393.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(32281..33519)
                     /gene="alaC"
                     /locus_tag="ACHK3A_000243"
     CDS             complement(32281..33519)
                     /gene="alaC"
                     /locus_tag="ACHK3A_000243"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO8738394.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYGIEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            33896..35593
                     /gene="ypdA"
                     /locus_tag="ACHK3A_000244"
     CDS             33896..35593
                     /gene="ypdA"
                     /locus_tag="ACHK3A_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO8738395.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            35608..36342
                     /gene="ypdB"
                     /locus_tag="ACHK3A_000245"
     CDS             35608..36342
                     /gene="ypdB"
                     /locus_tag="ACHK3A_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO8738396.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            36355..37212
                     /gene="ypdC"
                     /locus_tag="ACHK3A_000246"
     CDS             36355..37212
                     /gene="ypdC"
                     /locus_tag="ACHK3A_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8738397.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPVRQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(37215..39710)
                     /gene="ptsP"
                     /locus_tag="ACHK3A_000247"
     CDS             complement(37215..39710)
                     /gene="ptsP"
                     /locus_tag="ACHK3A_000247"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO8738398.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDGCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLNLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQMLADKRQQQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(39735..40772)
                     /gene="ypdE"
                     /locus_tag="ACHK3A_000248"
     CDS             complement(39735..40772)
                     /gene="ypdE"
                     /locus_tag="ACHK3A_000248"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO8738399.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSTIRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(40772..41857)
                     /gene="ypdF"
                     /locus_tag="ACHK3A_000249"
     CDS             complement(40772..41857)
                     /gene="ypdF"
                     /locus_tag="ACHK3A_000249"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO8738400.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVICR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIFADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQLKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGVEVLYAMPKTVLLTGEA"
     gene            complement(41872..43119)
                     /gene="fryC"
                     /locus_tag="ACHK3A_000250"
     CDS             complement(41872..43119)
                     /gene="fryC"
                     /locus_tag="ACHK3A_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO8738401.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(43141..43467)
                     /gene="fryB"
                     /locus_tag="ACHK3A_000251"
     CDS             complement(43141..43467)
                     /gene="fryB"
                     /locus_tag="ACHK3A_000251"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO8738402.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(43686..44651)
                     /gene="glk"
                     /locus_tag="ACHK3A_000252"
     CDS             complement(43686..44651)
                     /gene="glk"
                     /locus_tag="ACHK3A_000252"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO8738403.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKIEVQDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            44855..46111
                     /gene="yfeO"
                     /locus_tag="ACHK3A_000253"
     CDS             44855..46111
                     /gene="yfeO"
                     /locus_tag="ACHK3A_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO8738404.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     QMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            46226..46552
                     /gene="ypeC"
                     /locus_tag="ACHK3A_000254"
     CDS             46226..46552
                     /gene="ypeC"
                     /locus_tag="ACHK3A_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO8738405.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(46693..47931)
                     /gene="mntH"
                     /locus_tag="ACHK3A_000255"
     CDS             complement(46693..47931)
                     /gene="mntH"
                     /locus_tag="ACHK3A_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO8738406.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            48267..49469
                     /gene="nupC"
                     /locus_tag="ACHK3A_000256"
     CDS             48267..49469
                     /gene="nupC"
                     /locus_tag="ACHK3A_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO8738407.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(49518..51707)
                     /gene="pdeA"
                     /locus_tag="ACHK3A_000257"
     CDS             complement(49518..51707)
                     /gene="pdeA"
                     /locus_tag="ACHK3A_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO8738408.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLARMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(51915..51990)
                     /locus_tag="ACHK3A_000258"
     tRNA            complement(51915..51990)
                     /locus_tag="ACHK3A_000258"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51955..51957),aa:Ala,seq:ggc)
     gene            complement(52030..52105)
                     /locus_tag="ACHK3A_000259"
     tRNA            complement(52030..52105)
                     /locus_tag="ACHK3A_000259"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52070..52072),aa:Ala,seq:ggc)
     gene            52326..52685
                     /gene="yfeC"
                     /locus_tag="ACHK3A_000260"
     CDS             52326..52685
                     /gene="yfeC"
                     /locus_tag="ACHK3A_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO8738409.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            52687..53079
                     /gene="yfeD"
                     /locus_tag="ACHK3A_000261"
     CDS             52687..53079
                     /gene="yfeD"
                     /locus_tag="ACHK3A_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003834896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO8738410.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESE"
     gene            complement(53130..54545)
                     /gene="gltX"
                     /locus_tag="ACHK3A_000262"
     CDS             complement(53130..54545)
                     /gene="gltX"
                     /locus_tag="ACHK3A_000262"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO8738411.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            54804..54879
                     /locus_tag="ACHK3A_000263"
     tRNA            54804..54879
                     /locus_tag="ACHK3A_000263"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54837..54839,aa:Val,seq:tac)
     gene            54924..54999
                     /locus_tag="ACHK3A_000264"
     tRNA            54924..54999
                     /locus_tag="ACHK3A_000264"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54957..54959,aa:Val,seq:tac)
     gene            55046..55121
                     /locus_tag="ACHK3A_000265"
     tRNA            55046..55121
                     /locus_tag="ACHK3A_000265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55079..55081,aa:Val,seq:tac)
     gene            55126..55201
                     /locus_tag="ACHK3A_000266"
     tRNA            55126..55201
                     /locus_tag="ACHK3A_000266"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55159..55161,aa:Lys,seq:ttt)
     gene            complement(55312..56196)
                     /gene="xapR"
                     /locus_tag="ACHK3A_000267"
     CDS             complement(55312..56196)
                     /gene="xapR"
                     /locus_tag="ACHK3A_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO8738412.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(56237..56395)
                     /locus_tag="ACHK3A_000268"
     CDS             complement(56237..56395)
                     /locus_tag="ACHK3A_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO8738413.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(56448..57704)
                     /gene="xapB"
                     /locus_tag="ACHK3A_000269"
     CDS             complement(56448..57704)
                     /gene="xapB"
                     /locus_tag="ACHK3A_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO8738414.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(57765..58598)
                     /gene="xapA"
                     /locus_tag="ACHK3A_000270"
     CDS             complement(57765..58598)
                     /gene="xapA"
                     /locus_tag="ACHK3A_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO8738415.1"
                     /translation="MSQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            58846..59610
                     /gene="yfeN"
                     /locus_tag="ACHK3A_000271"
     CDS             58846..59610
                     /gene="yfeN"
                     /locus_tag="ACHK3A_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO8738416.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(59648..60574)
                     /gene="yfeR"
                     /locus_tag="ACHK3A_000272"
     CDS             complement(59648..60574)
                     /gene="yfeR"
                     /locus_tag="ACHK3A_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8738417.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFILHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWEVVRGQGRALMAGREGDPLYQI"
     gene            60664..61662
                     /gene="yfeH"
                     /locus_tag="ACHK3A_000273"
     CDS             60664..61662
                     /gene="yfeH"
                     /locus_tag="ACHK3A_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO8738418.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(61659..61877)
                     /gene="ypeB"
                     /locus_tag="ACHK3A_000274"
     CDS             complement(61659..61877)
                     /gene="ypeB"
                     /locus_tag="ACHK3A_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO8738419.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(61879..63894)
                     /gene="ligA"
                     /locus_tag="ACHK3A_000275"
     CDS             complement(61879..63894)
                     /gene="ligA"
                     /locus_tag="ACHK3A_000275"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO8738420.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHEVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAVKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(63965..64963)
                     /gene="zipA"
                     /locus_tag="ACHK3A_000276"
     CDS             complement(63965..64963)
                     /gene="zipA"
                     /locus_tag="ACHK3A_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO8738421.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQLVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            65193..65954
                     /gene="cysZ"
                     /locus_tag="ACHK3A_000277"
     CDS             65193..65954
                     /gene="cysZ"
                     /locus_tag="ACHK3A_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO8738422.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            66139..67110
                     /gene="cysK"
                     /locus_tag="ACHK3A_000278"
     CDS             66139..67110
                     /gene="cysK"
                     /locus_tag="ACHK3A_000278"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO8738423.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            67494..67751
                     /gene="ptsH"
                     /locus_tag="ACHK3A_000279"
     CDS             67494..67751
                     /gene="ptsH"
                     /locus_tag="ACHK3A_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO8738424.1"
                     /translation="MFQQEVTITAPNGLHTRPAALFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            67796..69523
                     /gene="ptsI"
                     /locus_tag="ACHK3A_000280"
     CDS             67796..69523
                     /gene="ptsI"
                     /locus_tag="ACHK3A_000280"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO8738425.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     TDLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            69564..70073
                     /gene="crr"
                     /locus_tag="ACHK3A_000281"
     CDS             69564..70073
                     /gene="crr"
                     /locus_tag="ACHK3A_000281"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO8738426.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(70115..70966)
                     /gene="pdxK"
                     /locus_tag="ACHK3A_000282"
                     /gene_synonym="thiJ"
     CDS             complement(70115..70966)
                     /gene="pdxK"
                     /locus_tag="ACHK3A_000282"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO8738427.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            71071..71439
                     /gene="yfeK"
                     /locus_tag="ACHK3A_000283"
     CDS             71071..71439
                     /gene="yfeK"
                     /locus_tag="ACHK3A_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO8738428.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDDHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(71442..72353)
                     /gene="cysM"
                     /locus_tag="ACHK3A_000284"
     CDS             complement(71442..72353)
                     /gene="cysM"
                     /locus_tag="ACHK3A_000284"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO8738429.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MLAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPFAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(72487..73584)
                     /gene="cysA"
                     /locus_tag="ACHK3A_000285"
     CDS             complement(72487..73584)
                     /gene="cysA"
                     /locus_tag="ACHK3A_000285"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO8738430.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(73574..74449)
                     /gene="cysW"
                     /locus_tag="ACHK3A_000286"
     CDS             complement(73574..74449)
                     /gene="cysW"
                     /locus_tag="ACHK3A_000286"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO8738431.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(74449..75282)
                     /gene="cysT"
                     /locus_tag="ACHK3A_000287"
     CDS             complement(74449..75282)
                     /gene="cysT"
                     /locus_tag="ACHK3A_000287"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO8738432.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(75282..76298)
                     /gene="cysP"
                     /locus_tag="ACHK3A_000288"
     CDS             complement(75282..76298)
                     /gene="cysP"
                     /locus_tag="ACHK3A_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO8738433.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(76456..77247)
                     /gene="ucpA"
                     /locus_tag="ACHK3A_000289"
     CDS             complement(76456..77247)
                     /gene="ucpA"
                     /locus_tag="ACHK3A_000289"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO8738434.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(77376..78233)
                     /gene="murR"
                     /locus_tag="ACHK3A_000290"
     CDS             complement(77376..78233)
                     /gene="murR"
                     /locus_tag="ACHK3A_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO8738435.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKEQALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSELVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            78397..79293
                     /gene="murQ"
                     /locus_tag="ACHK3A_000291"
     CDS             78397..79293
                     /gene="murQ"
                     /locus_tag="ACHK3A_000291"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO8738436.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSQEGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGPEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            79297..80721
                     /gene="murP"
                     /locus_tag="ACHK3A_000292"
     CDS             79297..80721
                     /gene="murP"
                     /locus_tag="ACHK3A_000292"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO8738437.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            80726..82027
                     /gene="pbp4b"
                     /locus_tag="ACHK3A_000293"
     CDS             80726..82027
                     /gene="pbp4b"
                     /locus_tag="ACHK3A_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO8738438.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSALMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDAIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPCSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSDTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(82085..82984)
                     /gene="yfeX"
                     /locus_tag="ACHK3A_000294"
     CDS             complement(82085..82984)
                     /gene="yfeX"
                     /locus_tag="ACHK3A_000294"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO8738439.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSIHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(83080..83655)
                     /gene="yfeY"
                     /locus_tag="ACHK3A_000295"
     CDS             complement(83080..83655)
                     /gene="yfeY"
                     /locus_tag="ACHK3A_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO8738440.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(83716..84165)
                     /gene="yfeZ"
                     /locus_tag="ACHK3A_000296"
     CDS             complement(83716..84165)
                     /gene="yfeZ"
                     /locus_tag="ACHK3A_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 family protein"
                     /protein_id="HFO8738441.1"
                     /translation="MKSTEFHPVHYDAYGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(84152..84577)
                     /gene="ypeA"
                     /locus_tag="ACHK3A_000297"
     CDS             complement(84152..84577)
                     /gene="ypeA"
                     /locus_tag="ACHK3A_000297"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO8738442.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            84791..85660
                     /gene="amiA"
                     /locus_tag="ACHK3A_000298"
     CDS             84791..85660
                     /gene="amiA"
                     /locus_tag="ACHK3A_000298"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO8738443.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            85664..86563
                     /gene="hemF"
                     /locus_tag="ACHK3A_000299"
     CDS             85664..86563
                     /gene="hemF"
                     /locus_tag="ACHK3A_000299"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO8738444.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGTEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEDDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPEDGSPEAALSEFIKVRDWV"
     gene            complement(86497..87621)
                     /gene="eutR"
                     /locus_tag="ACHK3A_000300"
     CDS             complement(86497..87621)
                     /gene="eutR"
                     /locus_tag="ACHK3A_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO8738445.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSSG"
     gene            complement(87667..88167)
                     /gene="eutK"
                     /locus_tag="ACHK3A_000301"
     CDS             complement(87667..88167)
                     /gene="eutK"
                     /locus_tag="ACHK3A_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO8738446.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(88180..88839)
                     /gene="eutL"
                     /locus_tag="ACHK3A_000302"
     CDS             complement(88180..88839)
                     /gene="eutL"
                     /locus_tag="ACHK3A_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO8738447.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(88849..89736)
                     /gene="eutC"
                     /locus_tag="ACHK3A_000303"
     CDS             complement(88849..89736)
                     /gene="eutC"
                     /locus_tag="ACHK3A_000303"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO8738448.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCTTPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(89757..91118)
                     /gene="eutB"
                     /locus_tag="ACHK3A_000304"
     CDS             complement(89757..91118)
                     /gene="eutB"
                     /locus_tag="ACHK3A_000304"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO8738449.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(91130..92533)
                     /gene="eutA"
                     /locus_tag="ACHK3A_000305"
     CDS             complement(91130..92533)
                     /gene="eutA"
                     /locus_tag="ACHK3A_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO8738450.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTEARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPVGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDEKDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(92530..93756)
                     /gene="eutH"
                     /locus_tag="ACHK3A_000306"
     CDS             complement(92530..93756)
                     /gene="eutH"
                     /locus_tag="ACHK3A_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO8738451.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(93973..95160)
                     /gene="eutG"
                     /locus_tag="ACHK3A_000307"
     CDS             complement(93973..95160)
                     /gene="eutG"
                     /locus_tag="ACHK3A_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO8738452.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSLVGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(95150..95986)
                     /gene="eutJ"
                     /locus_tag="ACHK3A_000308"
     CDS             complement(95150..95986)
                     /gene="eutJ"
                     /locus_tag="ACHK3A_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO8738453.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKTEGIY
                     AK"
     gene            complement(95997..>96101)
                     /locus_tag="ACHK3A_000309"
     CDS             complement(95997..>96101)
                     /locus_tag="ACHK3A_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO8738454.1"
                     /translation="TTMTITTPTGEGVTSARTFVRLRRCVLVDAFRIV"
BASE COUNT        24334 a        25291 c        23203 g        23273 t
ORIGIN      
        1 attcgatcac aagcggatca ttgttagtac aataagattg cataaattca acattgccag
       61 aaaaaaataa aggcccgata gattacacca gcgggccttg tgcatgcgta ttaaatcgct
      121 tttgccatct tcagattaca cgggctcatc tgccagttga atgtgccgtt tccgctttca
      181 tcaagcgtta cgctggcaat ggccgaggtg gtaaacatag gcggcgtttc gcccggacat
      241 aactcggcaa ccagataccc gactaagggt aagtgggaga tcaccagtac cgaagcaaca
      301 ccttcattgg tcaacgcctg caaataggca ctgaccagac caacatcgcc gcagggcgtt
      361 aactccggca gaacttctgc actggaaggc aggttcagac aatcccctac ttcttcaagt
      421 gtttgctcgg ctcgcaggaa cgggctcacc agaacgcgtt cgatttccac tttttgacct
      481 ttcagccagt tcgccatcag gcgagattcg tcacaaccgt tagtggtcag aggacgaacc
      541 gaatcactgg cggcatcgag ggctgcgtcg ccgtgacgca tgataaaaac ttgcatattg
      601 caccgctttt gttaaccaga ttcgcccgtt ttctttcatc tgaaaccaga gaaagaaaac
      661 ggtggccggc attgtgcctt atccattcac cgaatgaaac gctgtttttt acctcaatgg
      721 cgtaagtata gtcaatatgc gtttacattt tgagcaacac tccgccattc agcgcggatt
      781 catatagctt tgaccttctt attgcaggtc agttgcagtt gttttccaaa aactttcccc
      841 acgcttgctc atctcgacta aacgctcgca aggggtaaaa cgggaaccat actgcgtggc
      901 cagtcgttgc attattgcaa ccacttcacc cgcaccgaga gaatcgatgt agcggaacgg
      961 accaccgaga aatggcggaa aaccaatgcc aaataccgcg ccaatatctc cgtcacgcac
     1021 gctacggata acctgctcgt ccagacaacg tactgcttca ttcagcatca acatcacaca
     1081 ccgttcagct acctgcggtg cggagagtcg cccctgccct tgtgtgccaa tgagcgggta
     1141 aatggcggga tcgacctgtt ttttgctttt acgccctttc tgaccataaa gatagaaacc
     1201 ccggccattt tttctgcctt tgcgatcgtc gttcaaaatt gaagaaacaa catttgcagg
     1261 cgcgctaaaa cgttctccat aagcggcttc cagtacagga ataattttag tcccggtgtc
     1321 gattcctacc tcatccaaaa gttggattgg gcctaccgga aaaccaaatt tcaccagcgc
     1381 ggtatcaatg tgctctaccc gttcaccttc ggtcaacatg cggatagctt cattaatgta
     1441 aggcgctaag atgcgattga cgtaaaaccc ggctttgtca cgcacgacaa ttggcgtttt
     1501 gccctgtttt ttcgccagtt ttactgtggt ggcgatggtt tgcgccgatg taccggcatg
     1561 gggaataatt tctaccaacg gcattttttc caccggactg aagaaatgca ggccaataac
     1621 ttgctcaggt cgcgcggcgt gagcggcgat atcgccaatc ggtaaagatg acgtgttcga
     1681 agcaaagatg gtatgagcgg cgcaattttg ctcaacttcc gccaccattt gttgttttaa
     1741 ttcgagattt tcaaacaccg cttcaataat cagatcgcga tgggcaaagc cgcaatagtc
     1801 cgtcgttccg gagattaatg ccagctgtct gtcacgttcg ctggctttga gatgacgacg
     1861 gcgaactttg ccctccagct gatcccaact gtacttcagc gcatgattta tgccctgcgg
     1921 gttgatatct ttaattctga ccggaagccc cgctttacaa gcagtgacat aagcaatacc
     1981 gccgcccatc aagccaccac ctaaaattcc cacgctgttt aatggcgcag gcggcgcatc
     2041 actgccagga tctttcttca cgtccgtact ggcaaaaaag atactacgca gcgcctgcga
     2101 ttgcggcgtc atcaccagtt cgccaaaagc acgggcttca gcgtcatagc cgccactggt
     2161 gccctgcgct aatcccgttt cgacaacctc caggatgcgt tctgtcgccg gatagttacc
     2221 ttgagttttg tgttctgttt tcttaccgac cattttgaac agcagcgcac gacctaacgg
     2281 ccccgccaga atacgctcgc gtacaggtag agggcgtgaa gatgggcgat cctgctttgc
     2341 cagctcaaca gcggcttcca gtaaaatgga ttgcggaacg acgtcatcca ccagccccag
     2401 ctttaatgcc tgtttcgccc gaagttgttt tccagtgagg atcatctcca atgctgtgct
     2461 gacacctatc aggcgcggta aacgctgggt gccgcctgaa ccgggtaata atccaagttg
     2521 tacttcaggc aaaccgagca ccgttttagg atcgtcagta cacatgcgac cgtggcacgc
     2581 cagcgccaac tccagcccac cgcccaggca agcgccatga atagccgcga taaccggaac
     2641 gggcaaagca tgaatctccg ccatcaactg ttggccctgc cgcgccagtg cttccgcttc
     2701 ttgcgccgtt ttgcagttgg cgatcatgtt gatgtctgcg ccagcaatga agttgtctgg
     2761 tttcgcggag ataaacacca cgccgcgcaa ctctttgttt tcacggagtt gcttaataat
     2821 ggcgcgcacc tgcgaggcaa actccgcctt cagggtattc attttctcac ccggtacgtc
     2881 gatggtgata acagcaatgt tgtccagacg gacattaagg gtaaacgctg atgccatttc
     2941 cattattccg cctccacaac cattgccgcg ccaagcccac ccgcagcaca ggcggtcact
     3001 aaaccaaatc caccgccgcg acggcgaagt tcatgcaatg tctgggtaat catccgcgcg
     3061 ccggtcgccg cgaagggatg cccgtaagca atcgaaccgc caagcacgtt aaatttgcta
     3121 tcgtccattt cgccagtggc atgtgcacgc cccagtacat cacgagcaaa acgttcgcta
     3181 cccagcaact gaatattcgc cagtgtctga gctgcaaagg cttcgtgcat atcgagcaat
     3241 gtcagatcgc tcattgtcaa accggcacgt tccagcgcca gcggcgttga ccaggccgga
     3301 ccgagcaaca tgtcctgcca gacatctatc gcagtaaatg cgtaactgcg cagatacccc
     3361 agcggtacca gccctaattc tttcgcccgc gattcggtca tcaggatcac cgccgctgcg
     3421 ccatcggtca gcggcgtact gtttgctgcc gttaccgtcc cgtgtttacg atcaaacgcc
     3481 ggacgcaact ttgcgtaatc agcaagcgag gaattaccgc gaatattgtt gtcttcggca
     3541 agcggttgtt tataaggagg gataaaggca gtcatcacct cttctttgag tttaccgtct
     3601 gaccacgcct gagcggctcg ctgatgcgaa cggtgcgcta atgcatcttg ctgttcgcga
     3661 gtaataccgt aggttttcgc catttgttca gcggtgtcgc ccatccgcaa gccagtagag
     3721 tattccgcca ctgccggagg caccggcatt aagtcgcgca aacgcaggcg agagaagagt
     3781 ttcaggcgct ggctcatggt tcgagctttg ttgacatcaa ccagcacgcg cgccagtttt
     3841 ttactgacgc caattggcaa tacggaagag gaatcggccc caccggcaat ccccgcgcga
     3901 atagttcccg ccatcaggct ttctgcgacg tttgcaatcg cctggaaact ggtagcgcaa
     3961 gcgcggctga cgctgtaagc atcggtatgc acattcattc cggtaccgag aacaatttca
     4021 cgcgcaatgt tgggggcttc aggcatttgt acgacctgac caaaaaccag ttgttcaatc
     4081 acttcggcgg ggatctcgct gcgtgccagc agttcgccta ccaccatctt ccccaaatca
     4141 acagcaggaa tgccatgaaa agccgtcgcc tggcgggcaa aaggcgtacg taaaccgcta
     4201 acaatggcga tacgatcgcc ctggcgggta accagcggta aaacctgacc cataacactc
     4261 ccctgtaaaa taataaataa agtggtctga cctgatcata gtcttaacca tttttttaca
     4321 tttagccaag tggagaaaag ggaaagtggg agctatgaca cagagagaaa agtagaagag
     4381 aaaagaaaac gcccctgcca gcaactgaca ggggcgtatg tacccgagag gaattaacgc
     4441 agacccagct ggaagatcag catttcagct tcgcaggcaa aagtgaaatc gatatccagg
     4501 cgcacaccgt cagactcttc agtgaaagtc ggggtgattt tgcacggttc ggattccacg
     4561 ctacgggctt tttcagtcag cgccgccagc gtttgttctg cttctgcgcg gtttgcaaac
     4621 acacggctgt aagacgcggt gcagtcggag ttgtccataa tggtgccaac atccatacag
     4681 cagcaaaccg gggtttcatc agcactacat ttactcatcg ttgatttcct ctgtatgtgc
     4741 acccaaggtg ccagataaac gttgtggata ttttacgctt ccggaaagtg ctgctccagt
     4801 tgttaattct gcaaaatcgg ataagtgacc gaaatcacac ttaaaaatga tctaaaacaa
     4861 aattcacccg aatccatgag tgcgccacct cccaattttg ccagctggat cgcgtttctt
     4921 agatcatatc tgaaaaaaga tagaaacata cttgcaacat tccagctggt ccgacctata
     4981 ctctcgccac tggtctgatt tctaagatgt acctcagacc ctacacttcg cgctcctgtt
     5041 acagcacgta acatagtttg tataaaaata aatcattgag gttatggtca tgagccagaa
     5101 aaccctgttt acaaagtctg ctctcgcagt cgcagtggca cttatctcca cccaggcctg
     5161 gtcggcaggc tttcagttaa acgaattttc ttcctctggc ctgggccggg cttattcagg
     5221 ggaaggtgca attgccgatg atgcaggtaa cgttagccgt aaccccgcat tgattaccat
     5281 gtttgaccgc ccgacatttt ctgcgggtgc ggtttatatt gatccggatg taaatatcag
     5341 cggaacgtct ccgtctggtc gtagcctgaa agccgataac atcgcgccta cggcatgggt
     5401 tccgaacatg cactttgttg caccgattaa cgaccaattt ggttgggggg cttcaattac
     5461 ctctaactat ggtctggcga cagagtttaa cgatacttat gcaggcggtt ctgtcggtgg
     5521 taccaccgac ctcgaaacca tgaacctgaa cttaagcggt gcatatcgct taaataatgc
     5581 atggagcttt ggtcttggtt tcaacgccgt ctacgctcgc gcgaaaattg aacgtttcgc
     5641 aggcgatctg gggcaactgg tggctggtca gattaggcaa tctcctgctt ggctgactcc
     5701 tcaggggcaa gcattagcag ctaccgccaa cggcatcgac agtaatacca aaatcgctca
     5761 cctgaacggt aaccagtggg gctttggctg gaacgccggt atcctgtatg aactggataa
     5821 aaacaaccgc tacgcattga cctaccgctc tgaagtgaaa attgacttca agggtaacta
     5881 cagcagcgat cttaacccgg cttttaataa ctacggtttg ccaattccta ccgcgacagg
     5941 tggcgcaacg cagtcgggtt atctgacgct gaacctgcct gaaatgtggg aagtgtcggg
     6001 ttataaccgt gttgatccgc agtgggcaat tcactatagc ctggcttaca ccagctggag
     6061 tcagttccag cagctgaaag cgacctcaac gagtggcgac acgctgttcc agaaacatga
     6121 aggctttaaa gatgcttacc gcatcgcgtt gggaaccact tattactacg atgataactg
     6181 gaccttccgt accggtatcg cctttgatga cagcccagtt ccggcacaga atcgttctat
     6241 ctccattccg gaccaggacc gtttctggct gagtgcaggt acgacttacg catttaataa
     6301 agatgcttca gtcgatgttg gtgtttctta tatgcacggt cagagcgtga aaattaacga
     6361 aggcccatac cagttcgagt ctgaaggtaa agcctggctg ttcggtacta actttaacta
     6421 cgcgttctga taacgcgttc gcttaataag aaaaaaggtg agttttgcga ctcacctttt
     6481 ttgtttgttt cttattcaga atcgatatct tttaaatcat cctgaatcgc ttgcgcgttc
     6541 ggattttcct gcggtttgag ttcgccgcca ttagcgatga aatcatgacg ctggaagtac
     6601 gcttcgcgca ccataatata aggatcggac gactgacgca gcagaccatc ggaatccaac
     6661 aactgcgcac gagtttctat cccttcaagc gtccatttac ccacagacat cggccaggtc
     6721 agccaggaaa gaaccgggta aagaccatcc gccatatcac caccgtcatc acgcagcgtg
     6781 aagctaccgt agaacggtaa ctgaacgtat ggcccataac ccacaccata atgaccaagc
     6841 gtactaccga agcggtgagg ttcagtccgt tgcagtttcg ggttcgccat ccctgcaaca
     6901 tcaataaagc cgcccatccc caaaatggtg ttcaggaaaa agcgggtaaa gtggaccatc
     6961 ccctgataag ggtcgccctg caagaagtag ttaaccatca ccgcaggttc ttcaaggttg
     7021 ccagtaaagt tgctcaaacc gttacgcgcc ggttgcggaa cataatcacg ccaggcgaca
     7081 gcgaccggtc gaacaatata cgggtctaat acattgaaat tgaagttgta catggtgcgg
     7141 ttgaaccctt ctagcgggtc agaacgccct tgctgatctg taccggaact cgcacacccc
     7201 accagaagcg tagttcccag agcaagcgcc gacaggcgaa gcttcataaa tgtctccctg
     7261 tttttttatg gcttatgcag tttgccatcc atgacggaac gataccgtat ccgcctgttt
     7321 aggtgtgggc gattgtaaca gcacgtcaac tgatgtccag acgccctgat ttgctgattt
     7381 gatcatagcc tggtaatcgc cgccctgtag gctacttgat tctatagaaa cagaaaaagg
     7441 caaacgcagc cttttctaca ttttcagagt aactcccgcc agttgcgagc aaaaaagccg
     7501 ctacgcttta gctatacgtg ctaattcaag agaagagacc atggacaacg acaaaattga
     7561 tcaacacagc gacgaaattg aagttgagag cgaagaaaaa gagcgcggca aaacaataga
     7621 aatagatgaa gatcgactcc cctcccgggc gatggcaatt catgagcata tccgccagga
     7681 tggtgaaaaa gagctggaac gcgacgcaat ggcactactg tggtcagcca ttgcggcggg
     7741 tctgtcgatg ggcgcttcgc tactggcaaa agggatattt catgtcgaac tggaaggcgt
     7801 gccgggcagc ttcttgctgg agaatctcgg ttataccttt ggttttatta tcgtcattat
     7861 ggcccgccag caattattta ccgaaaacac cgtgactgcg gtactacccg tcatgcaaaa
     7921 accgacaatg agcaacgttg gcttacttat gcggttatgg ggcgtcgtgc tgctgggtaa
     7981 tattctcggg acaggtattg cggcgtgggc atttgaatat atgccaatct tcaatgaaga
     8041 aactcgcgat gcatttgtca aaatcggcat ggatgtgatg aagaacaccc ccagcgagat
     8101 gtttgccaac gcgatcattt ccggctggct gatcgccact atggtttgga tgtttcctgc
     8161 agcgggtgcg gcaaagattg tggtgattat attgatgacc tggcttattg ccctgggtga
     8221 caccactcac atcgttgtcg gttctgttga aatcctctat ctggtgttta acggtacgct
     8281 gcactggagc gatttcatct ggcccttcgc actacctact ttagcgggga acatctgcgg
     8341 cggcaccttt atcttcgcgt taatgagtca tgcacagatc cgtaacgaca tgagcaacaa
     8401 gcgcaaagca gaagcacgcc aaaaagcaga acgtgcggaa aacattaaga aaaatgataa
     8461 aaacccggca taaatggcga gggtttaagc aatcgagcgg cagcgtactt accccgcagt
     8521 ccattagcgg gtatactcat gccgcattgt cctcttagtt aaatggatat aacgagcccc
     8581 tcctaagggc taattgcagg ttcgattcct gcaggggaca ccagataccc ttcaaaccaa
     8641 atctaccttc accccgtaaa agatgggttt ggcagcacac ttgccctata tctactcatt
     8701 tttactgcaa caggttgaaa tctcagcact gtcagaaagc gctgatgact aaacagccct
     8761 gggccgggcg atgtaaccat catacagaat cctgatagcg aaatatggcg tgactcgata
     8821 cttcactctg caatgcattc cttgatgaat tcgcaggccc gtgatacacg ggacacgtcg
     8881 ctgaatgacg acaatgtcct ggaaatcagc gaaccgcgta tccggagtac atttgagcga
     8941 ctgtaccaga acatgaatga ggcgtttgga ttaggtgatt attagcaggg gtaagcattt
     9001 ttgtattatt attttccggt tgagggatat ggagatatcg acaacaaccg gaaaaagttt
     9061 acgtctatat tgctgaaggt acaggcgttt ccataactat ttgctcgcgt tttttactca
     9121 ggaagaaaat gccaaatagc aacatcaggc agacaatacc cgaaattgcg aagaaaactg
     9181 tctggtagcc tgcgtggtca aagagtatcc cagtcggcgt tgaaagcagc acaatcccaa
     9241 gcgaactggc aatttgaaaa ccaatcagaa agatcgtcga cgacaggcgc ttatcaaagt
     9301 ttgccacgct gtatttgaag acggatatga cacaaagtgg aacctcaatg gcatgtaaca
     9361 gcttcactaa tgaaataatc caggggttaa cgaacagcgc gcaggaaagg atacgcaacg
     9421 ccataatcac aacaccgata agtaatgcat tttttggccc tacccgattc acaaagaaag
     9481 gaataatcgc catgcacagc gcttcgagta ccacctggaa tgagttgaga taaccataca
     9541 ggcgcgttcc tacatcgtgt gattcgaata aacctgcata aaagacagga aagagttgtt
     9601 gatcaaaaat gttatagaaa gaccacgtcc ccacaataaa tatgacaaaa acccagaagt
     9661 ttcgatcctt gaaaactgcg ataaaatcct ctttttttac ccctcccgca tccgccgcta
     9721 tgcactggtg atccttatct ttaaaacgca tgttgatcat cataaataca gcgccaaata
     9781 gcgagaccaa ccagaagttg atatggggac tgatactaaa aaatatacca gcaaagaacg
     9841 cgccaatagc atagccaaaa gatccccagg cgcgcgctgt tccatattcg aaatgaaaat
     9901 ttcgcgccat tttttcggtg aagctgtcaa gcaaaccgca tcccgccaga taccccaggc
     9961 caaaaaagag cgcccccaga attagaccta cagaaaaatt gctttgcagt aacggttcat
    10021 aaacgtaaat cataaacggt ccggtcaaga ccagaatgaa actcatacac cagatgagcg
    10081 gtttcttcag accgagttta tcctgaacga tgccgtagaa catcataaat agaatgctgg
    10141 taaactggtt gaccgaataa agtgtaccta attccgtccc tgttaatcct agatgtcctt
    10201 tcagccaaat agcgtataac gaccaccaca gcgaccagga aataaaaaag agaaatgagt
    10261 aactggatgc aaaacgatag tacgcatttc tgaatggaat attcagtgcc ataattacct
    10321 gcctgtcgtt aaaaaattca tgtcctattt agagataaga gcggcctcgc cgtttacttc
    10381 tcactattcc agttcttgtc gacatggcag cgctgtcatc gcccctttcg ccgttactgc
    10441 aagcgctccg caacgttgag cgagatcgat aattcgtcgc atttctcttt catctgtaga
    10501 taatcccgta gaggacagac ctgtgagtaa cccggcaacg aacgcatctc ccgcccccgt
    10561 gctatcgaca caattcacag acattccagc aaaatggtga acttgtcctc gataacagac
    10621 caccacccct tctgcacctt tagtcaccaa cagcatggcg atctcatact cttttgccag
    10681 ggcgcatata tcccaatcgt tctgtgtttt tccactgata agtcgccatt cttcttccga
    10741 gagcttgacg acatccgcca gttgtagcgc ctgccgcaaa cacaagcgga gcaaatgctc
    10801 gtcttgccat agatcttcac gaatattggg atcgaagctg acaaaacctc cggcatgccg
    10861 gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc tcggcagaca acgcaattga
    10921 acagagatgt aaccattcgc catgtcgcca gcagggcaag tctgtcgtct ctaaaaaaag
    10981 atcggcactg gggcggacca taaacgtaaa tgaacgttct ccttgatcgt tcagatcgac
    11041 aagcaccgtg gatgtccggt gccattcatc ttgcttcaga tacgtgatat cgacaccctc
    11101 agttagcagc gttctttgca ttaacgcacc aaaaggatca tcaccgaccc gacctataaa
    11161 cccacttgtt ccgcctaatc tggcgattcc caccgcaacg ttagctggcg cgccgccagg
    11221 acaaggtagt agccgcccgt ctgattctgg caagagatct acgaccgcat cccctaaaac
    11281 ccatactttg gctgacattt ttttccctta aattcatctg acttacgcat agtgataaac
    11341 ctctttttcg caaaatcgtc atggatttac taaaacatgc atattcgatc acaaaacgtc
    11401 atagttaacg ttaacatttg tgatattcat cgcatttatg aaagtaaggg actttatttt
    11461 tataaaagtt aacgttaaca attcacccaa tttgcttaac caggatgata aaaatgacgc
    11521 aatctcgatt acatgcggcg caaaacgcac tagcaaaact tcacgagcgc cgaggtaaca
    11581 ctttctatcc ccattttcac ctcgcgcctc ccgccgggtg gatgaacgat ccaaacggcc
    11641 taatctggtt taacgatcgt tatcacgcgt tttatcaaca tcacccgatg agcgaacact
    11701 gggggccaat gcactgggga catgccacca gcgacgatat gatccactgg cagcatgagc
    11761 ctattgcgct agcgccagga gacgagaatg acaaagacgg gtgtttttca ggtagtgctg
    11821 tcgatgacaa tggtgtcctc tcacttatct acaccggaca cgtctggctc gatggtgcag
    11881 gtaatgacga tgcaattcgc gaagtacaat gtctggctac cagtcgggat ggtattcatt
    11941 tcgagaaaca gggtgtgatc ctcactccac cagaaggcat catgcacttc cgcgatccta
    12001 aagtgtggcg tgaagccgac acatggtgga tggtagtcgg ggcgaaagat ccaggcaaca
    12061 cggggcagat cctgctttat cgcggcagtt cattgcgtga atggactttc gatcgcgtac
    12121 tggcccacgc tgatgcgggt gaaagctata tgtgggaatg tccggacttt ttcagccttg
    12181 gcgatcagca ttatctgatg ttttccccgc agggaatgaa tgccgaggga tacagttacc
    12241 gaaatcgctt tcaaagtggc gtaatacccg gaatgtggtc gccaggacga ctttttgcac
    12301 aatccgggca ttttactgaa cttgatagcg ggcatgactt ttatgcacca caaagctttt
    12361 tagcgaagga tggtcggcgt attgttatcg gctggatgga tatgtgggaa tcgccaatgc
    12421 cctcaaaacg tgaaggctgg gcaggctgca tgacgctggc gcgcgagcta tcagagagca
    12481 atggcaaact tctacaacgc ccggtacacg aagctgagtc gttacgccag cagcatcaat
    12541 ctatctctcc ccgcacaatc agcaataaat atgttttgca ggaaaacgcg caagcagttg
    12601 agattcagct gcagtgggcg ttgaagaaca gtgatgccga acattacgga ttacagctcg
    12661 gcactggaat gcggctgtat attgataacc aatctgagcg acttgttttg tggcggtatt
    12721 acccacacga gaatttagat ggctaccgta gtattcccct cccgcagggt gacatgctcg
    12781 ccctaaggat atttatcgat acatcatccg tggaagtatt tattaacgac ggggaggcgg
    12841 tgatgagtag ccgaatatat ccgcagccag aagaacggga actgtcgctc tatgcctccc
    12901 acggagtggc tgtgctgcaa catggagcac tctggcaact gggttaacat aatatcaggt
    12961 ggaacaacgg atcaacagcg ggcaagggat ccgcgtcact cttccccctt cacgatcttc
    13021 aataatatgc aatgcagctt cccgcccgat aatgtcatgt ggaagctgaa ttgtggtcag
    13081 cggcggtaaa aacagatgcc cgacgccaac cagattatca aagcccatta cggcgacatc
    13141 ctgcgggata cgtaccccct tcgccaggag aacctgataa gcgacaaagg ctgcgcgatc
    13201 gttaccacat atcagaacat caaaatctgg tttgcccgat ttgaagtggt cattgagtaa
    13261 acttgcgaga tcggtgtagt gatcatcacc tgttgccatg tgaaattgtt tcacctcagc
    13321 cagatctcgt ccagcatcac gccaggcctg ctcaaatccc tgccgacgat accctgttgc
    13381 caacgcactt tccggtagcc agaagcataa cggttgacga tagcccgccg cgagcaaatg
    13441 ctgtgttgat tcatattgtg cagtgtaatc atcagggata taactgggta acgctgggtc
    13501 atccgccaca cagttcgcca atacaatatt ttcaccatac agagactcag gcagcgagat
    13561 atgtcgcagc cccattgtag tatagataat gccatccgga cggtgggcaa gcagctgacg
    13621 tgccgcgcgg gcagcgtcat cttcagaaaa aatattgatt aaaaaactat tccagccaaa
    13681 ctcgctggcg gtttgctcaa tggcaagcag aatatcaaca gagaaaggag tggtggccgt
    13741 gtcctgcgcc agcacggcga gagtcgacgg cttacgtcct tgagcgcgca tcttacgggc
    13801 ggaaagatca ggaacataat tcagggtctg gattgcctgc aatacgcggt cacgcgttgc
    13861 aggacgcaca gattctgcat tatgcatcac ccgggagact gtcatcatcg acactcccgc
    13921 caggcgtgcg acatccttta atgaagccat acccaagccg tttgccgtaa aacgggcact
    13981 gtagcagaaa cagacgtcac tggcgagatc caacgatatt ctcaacgccc tatcacccga
    14041 cacggcaata caataaaaaa taacaataat tcccggacaa ttgtacccaa ttccgcctct
    14101 gttctcgcca acgagtaacc atgtaggcaa gtaggtaaaa catgccaact ggcatcgcca
    14161 acctggagaa gaaaatataa atatggatca ttgtgctgcc gctgtatccc gacatcagtc
    14221 cggaaattat tgcgattgct atcggttcag gtgcgattgg ctgcacgatc gttacggact
    14281 cgcttttctg gctggtgaag caatattgcg gcgctacgct caatgaaaca tttaaatact
    14341 atacgacagc gacatttatc gcttcagtcg tcgctctggc gggcacattc ctgctgtcat
    14401 ttatcatcta agcgcaaaga gacgtactat ggaaaacgct aaaatgaact cgctcatcgc
    14461 ccagtatccg ttggtaaagg atctggttgc tcttaaagaa accacctggt ttaatcctgg
    14521 cacgacctca ttggctgaag gtttacctta tgttggcctg accgaacagg atgttcagga
    14581 cgcccatgcg cgcttatccc gttttgcacc ctatctggca aaagcatttc ctgaaactgc
    14641 tgctactggg gggattattg aatcagaact ggttgccatt ccggctatgc aaaaacggct
    14701 ggaaaaggaa tatcaacaac cgatcagcgg gcaactgcta ctgaaaaaag atagccattt
    14761 gcccatttcc ggctccataa aagcacgcgg cgggatttat gaagtcctgg cacatgcaga
    14821 aaaactggct ctggaagcgg ggttgctgac gcttgaagat gactacagca aactgctttc
    14881 tccggagttt aaacagttct ttagtcaata cagcattgct gtgggctcaa ccggaaatct
    14941 ggggttatca atcggcatta tgagcgcccg cattggcttt aaggtgacag tacatatgtc
    15001 tgctgatgcc cgggcatgga aaaaagcgaa actgcgcagc catggcgtta cggtcgtgga
    15061 atatgagcaa gattatggtg ttgcagtcga ggaaggacgt aaagcagcgc aatctgaccc
    15121 gaactgtttc tttattgatg acgagaattc ccgcacgttg ttccttgggt attccgtcgc
    15181 tggccagcgt cttaaagcgc aatttgccga gcaaggtcgt attgtcgatg ctgataaccc
    15241 tctgtttgtc tatctgccgt gtggtgttgg cggtggtcct ggtggcgtcg cattcgggct
    15301 taaactggca tttggcgatc atgttcactg cttttttgcc gaaccaacgc actccccttg
    15361 tatgctgtta ggcgtccata caggattaca cgatcagatt tctgttcagg atattggtat
    15421 cgataacctt accgcagcgg atggccttgc agttggtcgc gcatcaggct ttgtcgggcg
    15481 ggcaatggag cgtttactgg atggcttcta tacccttagc gatcaaacca tgtatgacat
    15541 gcttggctgg ctgacgcagg aagaaggtat tcgtcttgaa ccttcggcac tggcgggtat
    15601 ggccggacct cagcgcgtgt gtgcatcagt aagttaccaa cagatgcacg gtttcagcgc
    15661 agaacaactg cataatgcca ctcatctggt gtgggcgacg ggaggtggaa tggtgccgga
    15721 agaagagatg gagcaatatc tggcaaaagg ccgttcataa cgtttcaacg cagcatcgcc
    15781 atcctttccc tgggtgagcg atgctgccga tggcgcagac ttaagatccc cggtcttacc
    15841 tgctataatt ctctctttat accatcaccc aacgcctttc gctgtaaacg gcggtttcgc
    15901 aaaccaaacc aataccgtaa gtaagataaa agcaatagcc gccatacgga aaatctcatt
    15961 tgcagaaatt gaaagtgact gctgagttat ttcattatta atttcattaa gaactcctga
    16021 aagcgaacca tagtatttat ccataatttg tgacgatgaa ttaaatacgg ggttaaattg
    16081 atcgatggtt gctgtcaact gactatggtg taacgattcg cgtcgtcccc acagcgtcat
    16141 tgtcaacgac gtaccaactg atcctgacaa ggtacgaaaa aaattactca tactcgaggc
    16201 attggcaaat ttattatctg gcaaccctga aaacgaaatc gttgttaagg gtaaaaagaa
    16261 acaggcaacg gcgaatccct ggaaaaactg tggcaaaatg atgcctgtaa aatcaatcgt
    16321 tggcataaat gtcacagaac gccagtaata gcaaaccgca tacatcaaaa aactaaatgt
    16381 caccaacaac cgcatatcaa ttttgttgcc ataacgtcct atcaaaggtg atattaatag
    16441 cggcatgatg ccgatgggcg cataggcaag tccggcccat atcgcattat accccatcgt
    16501 ttcctggagt aactgcggca taaggactat cgccccagag taaaataaat acgcgcatgt
    16561 gatactcaca ataccaatgg tgaaattacg ggacttaaac aaactgagat caagaatcgg
    16621 attctctgag gtcgactccc aaatgactaa agaaatcaga gaaataactg acactactgt
    16681 tagtattatt attgtactcg agttgaacca atccagatcg cgccctttat caagcattat
    16741 ctgcaagcca ccaacaccga gcactaacag ggtcagtcct ggtagattca ttttgaccgg
    16801 tgaagtctca gtttctcttc ctttaagtaa ggttaagcat aatgtcagaa cgataatccc
    16861 catagggaca ttgattaaaa atatccaacc ccagctaaag ttatcgcaaa tatagccgcc
    16921 caatatcggc ccacatatcg gagcgataat tacggtcatt gaccataatg ccaaagcaaa
    16981 tgttcttttt tctggcggat aattccttaa taataaactc tgtgacagtg gaattaacgg
    17041 ccccgccatt aagccctgaa cgactctaaa aaatatcagc acatcaagat tggtcgatag
    17101 gctacacatt aatgaagata gtgaaaaaaa ggttactgaa agtaaaaata atcttaattc
    17161 gcctattctt tgtgccagcc tgccggtaac agggatcgca atggcatttg ctacgccaaa
    17221 cgaagtgata acccaggtgc cttcgtctgt tgatgctccc agaaagccag atattgtcgg
    17281 tattgcgacg ttagaaatag tggaatccaa catttgcata aacgtcgcta aggacaatgc
    17341 aatagtaacg caccataacg tcccaccggt taatggtgcc ggagttgatt tagtgattgc
    17401 cattaaagtt gtccattatg cgaaataatg ttgctaattt ctttttctat cggagtagta
    17461 tcgataacta aagccttact ggtataagca ggcatggagg tcacggttga agccagctca
    17521 ggcatctcag caatgtcttc gtccttcgta tcaatagttg cagtcatcga taaaccaata
    17581 cgtaagggat gttccatgag ttcttttgga tcaagagaaa cttcaaccgg tacacgctga
    17641 acgattttga tccagttccc tgtcgcattt tgtgcaggta ataaggagaa cgcattgccg
    17701 gttcccatat tgattcctgt cacccgacca tgaaacacaa cattttcacc ataaagatcg
    17761 ctgataatat tgaccgattg accaatccgt acatccgtga gttgtgtttc tttaaagttg
    17821 gcattaaccc acatttgacg tgccggtacg acagccatta acgattgtcc gggactcact
    17881 gtttcgccga cctgaacact tcgctgggca atatagccgg taacaggact cttaatatcc
    17941 gtacgtttaa gcgctaacca ggcttctttg gttgcatctg ccgcttcaac gacttgtggc
    18001 tgacggttta atggtgtgtt cattactaaa gctttattcg ctttataagc ctggatagcg
    18061 gcattcaatg ccgctttgct acttattaac gtatctttgg tatgctccag cgtttctttt
    18121 gaaataaccc cctgcttcgc taaaggcaca cgacggttat aatcttctaa agattgttga
    18181 tactgaatac gtgctgaagc gacttcggca ctgtattgtt tatcctgtaa gtacagttta
    18241 ttcgtttgcc gaacaatatt tgccagatta tttttagctt tattgagtgc gatagtggca
    18301 tcagtcttat ccagtgagac taaaatgtca ccctgtcgaa cgtagttcgt atctttatga
    18361 ttaacgacag tgacgctgcc tgaaacttgt gcagaaattg gatctgcatt ccccgtgaca
    18421 taggcgtcat ctgtactaat catgtcttct aattccattg accaataggc ataggcacct
    18481 gaaaacgcaa taaataaaac taccgccaat aaagaaaagt attttcttct gttagaatgt
    18541 tttttatttg aattaatctg ttccactttt atctctcatt tcgcatagat gaaatttatg
    18601 aattgaatga aaagagtatt cattcttcat atcaaacaca gtgcatgtgt tgcacattat
    18661 cccaggaacg ttggaggcct aatttaaatg aggtgcaaaa aaaacaacag aggactaaac
    18721 cgtggctttt gcaatacaat tcttacgcat gtaggattag tgagaagact tatagtgcta
    18781 atgtgaaact ataaatcatc ggtacaatcc ctgattttat tgttgacatt tcatttatgc
    18841 cgactattta tatggtatac ttgttgaatt atcttaaagg aagctcaaat tttcttattt
    18901 tgattgagaa aatgagatga tgccttatgt ctgtattact acagggagaa gggaaatgct
    18961 tcattgcaaa gggaataatc tatgaacgca ataattattg atgaccatcc tcttgctatc
    19021 gcagcaattc gtaatttatt gatcaaaaac gatattgaaa tcttagcgga gttgactgaa
    19081 gggggaagtg ccgttcagcg ggtggaaaca cttaaacctg atatcgtcat cattgatgtc
    19141 gatatccccg gagttaacgg tatccaggtg ttagaaacgc tgaggaagcg ccaatatagc
    19201 ggaattatta ttatcgtctc cgctaaaaat gaccattttt acgggaaaca ttgtgctgat
    19261 gctggcgcta atggtttcgt gagtaaaaaa gaaggcatga acaatatcat tgcagctatt
    19321 gaagctgcaa aaaatggcta ctgctatttc cccttctctc tcaaccggtt tgttggaagt
    19381 ttaacgtccg accagcaaaa actcgactcc ttatcgaaac aagaaattag tgtcatgcgg
    19441 tatattcttg atggcaagga taataatgac attgctgaaa aaatgttcat cagcaacaaa
    19501 actgtcagca cttataaaag tcgcttgatg gaaaaattag aatgcaaatc actgatggat
    19561 ctttacacat tcgcacaacg taacaaaatc ggctaaccac atgaagtttt taccctatat
    19621 ttttcttctc tgttgtggtc tttggtcgac cataagtttc gcagacggag attacatcga
    19681 atatcgtggc atcagtagta acaaccgtgt cacacttgat ccactacgtc tgagcaacaa
    19741 ggaattacgt tggctcgcga gcaaaaaaaa tcttgtgatt gcagtacaca agtcccaaac
    19801 agctacgttg ttgcataccg attcgcagca acggattcgt ggtattaatg ctgattattt
    19861 aaatctttta aaaagagcgt taaatatcaa attaacactc cgggaatacg cagatcatca
    19921 aaaagcaatg gacgcgctgg aagacggtga agttgatata gtgttatcac atttagttgc
    19981 ttcgccgcct cttaatgatg acattgctgc aaccaatcct ctgataatta cctttccggc
    20041 gctggtcact acccttcacg attcaatgcg accgcttacc tcatcaaaac cagtaaatat
    20101 tgctcgagta gcaaattatc ccccagacga ggtaattcat caatcatttc caaaagcaac
    20161 aattatctct tttacaaatt tatatcaggc attagcatcc gtctcagccg gacagaatga
    20221 ttactttatt ggtagtaaca tcattaccag cagtatgatt tcccgttatt ttactcactc
    20281 cttaaatgta gtgaagtatt ataactcacc gcgtcaatat aactttttat taaccagaaa
    20341 agattctatc gttcttaatg aagtactcaa tcgatttgtt gatgctttaa caaatgaagt
    20401 tcgctatgaa gtatcacaaa attggcttga tacaggaaat ctggcctttc tgaacaaacc
    20461 attagaactc actgaacatg aaaaacagtg gattaagcag catcccgatt taaaggtgct
    20521 agaaaatcct tactcgccac cctattctat gacagatgaa actggctcag ttcggggcgt
    20581 gatgggtgat attcttaata ttattacctt gcaaacaggt ttaaattttt ctccgatcac
    20641 cgtttcacac aatattcatg ctgggacaca gcttaacccc ggtggatggg atatattgcc
    20701 cgccgctatt tatagtgaag atcgagaaaa taatgtttca tttgctgaag ccttcataac
    20761 aacgccttac gtttttgtca tgcaaaaagc gcctgacagt gaacaaacat taaaaaaagg
    20821 aatgaaagtt gccattccct attattatga gcttcattcg caattaaaag agatgtatcc
    20881 ggaggtagag tggataaaag tcgataacgc cagcgctgca tttcacaagg ttaaagaagg
    20941 tgaacttgat gctctggtcg cgacacagtt aaattcgcgt tacatgatcg accattacta
    21001 tcctaatgaa ctttatcatt ttcttattcc cggcgttcag aatgcatcac tttcgttcgc
    21061 ttttcctcgc ggagaaccgg aacttaagga tattattaat aaagcactga atgcaattcc
    21121 cccaagcgaa gttctgcgcc tgaccgaaaa atggattaaa atgcccaatg tgaccattga
    21181 cacatgggac ctctatagcg agcaatttta tattgttacg acattatccg ttttattagt
    21241 tggcagtagc cttttatggg gattctacct gttacgctca gttcgtcgtc gtaaagttat
    21301 tcagggtgat ttagaaaacc aaatatcatt ccggaaagcg ctctcggatt ccttaccgaa
    21361 tccaacttat gttgtaaact ggcaaggtaa tgtcattagt cacaatagtg catttgaaca
    21421 ttatttcact gctgattact ataaaaatgc aatgttacca ttagaaaaca gtgaatcccc
    21481 ctttaaagat gttttttcta atacgcatga agtcacagca gaaacgaaag aaaaccgaac
    21541 aatatacaca caggtatttg aaattgataa tggcatcgag aaaagatgca ttaatcactg
    21601 gcatacctta tgtaatctgc cagcaagcga acatgctgtt tatatttgtg gttggcaaga
    21661 tattaccgag acgcgtgatt taattcatgc actcgaagta gagagaaata aagcgatcaa
    21721 tgcaactgtc gcaaaaagcc agtttctggc aacaatgagt cacgaaataa gaacaccaat
    21781 aagctccatt atgggcttcc tggaacttct gtcgggttct ggtcttagca aggaacaacg
    21841 ggtggaggcg atttcacttg cctacgccac cggacaatca ctcctcggct taattggtga
    21901 aatccttgat gtcgacaaaa ttgaatcggg taactatcaa cttcaaccac aatgggtcga
    21961 tatccctact ttagtccaga acacttgtca ctctttcggt gcgattgctg caagcaaatc
    22021 gatcgcatta agttgcagca gtacgcttcc tgaacgctac ctggttaaga tcgaccctca
    22081 ggcgtttaag caggtcttat ctaatttact gagtaatgct ctcaaattta ctaccgaggg
    22141 ggcagtaaaa attacgacct ccctgggtca cattgatgac aaccacgctg ttatcaaaat
    22201 gacgattatg gattctggaa gtggattatc gcaggaagaa caacaacaac tgtttaaacg
    22261 ctatagccaa acaagtgcag gtcgtcagca aacaggttct ggtttaggct taatgatctg
    22321 caaagaatta attaaaaaca tgcagggcga tttgtcatta gaaagtcatc caggcatagg
    22381 aacgatattt acgatcacaa tcccggtaga aattacccaa caagtggcgg ctgtcgaggc
    22441 aaaagcagaa caacctataa cactacctga aaagttgagc atattaatcg cggatgatca
    22501 tccgaccaac aggctattac tcaaacgcca gctaaatcta ttaggatatg atgttgatga
    22561 agccactgat ggtgtgcaag cgctacacaa agtcagtatg caacattatg atctgcttat
    22621 tactgacgtt aatatgccga atatggatgg ttttgagttg actcgcaaac tccgagagca
    22681 aaattcttcc ttacccatct gggggcttac agccaacgca caggctaacg aacgtgaaaa
    22741 agggttaaat tgcggcatga acttatgttt gttcaaaccg ttgactctgg atgtactgaa
    22801 aacacattta agtcagttac accaggttgc gcatattgca cctcagtatc gccaccttga
    22861 tatcgaggcc ctgaagaata atacggcgaa tgatctacaa ctgatgcagg agattctcat
    22921 gactttccag catgaaacgc ataaagatct acccgctgcg tttcatgcac tagaagctgg
    22981 cgataacaga actttccatc agtgtattca tcgcatccac ggtgcggcta acatcctgaa
    23041 tttgcaaaag ttgattaata ttagccacca gttagaaata acacccgttt cagatgacag
    23101 taagcctgaa attcttcagt tgctgaactc tgtaaaagaa cacattgcag agctggacca
    23161 ggagattact gttttctgtc agcaaaatga ctaaatagcg gctcccacaa tgttcaaatg
    23221 tgggagctat ttaccagcac atcttacatt tatgatgaga actcctggcg aatttgttcc
    23281 ccatgctggt cgagcgttgc cgctcccggc ataacatgcg ggtccgcgca gccgctgatt
    23341 tttatcggat tccccggcat cattattccc ccggcttcaa tcaacatatt tctcgcctga
    23401 gtttgtggca aattaatggc ctcagccaca cttaataacg gcgcgacggg tacaccaact
    23461 tcatgtattc tggctaacca aacttcagct gcctgcgttt ttaacgtccg ctcaatatat
    23521 tgtttaagaa tagcctggtt ttgtacgcgt aaaatattgc tgctaaatct gggatcatta
    23581 accagttccg taagctccag tgcctggcat aacgcagaaa aaagcttgtc attaccacaa
    23641 caaatcgtaa tcggcttatc ctgagtatcg aaaacatcaa aaggtgccat gtagggatgg
    23701 cgatttccca ggcgttgtgg tgacttccct gtcgcaatat atgccatcag accatgctcc
    23761 agaaaactca gcgtggcatc aaacatcgct atatcgacat gcgcccctct ttggctcttt
    23821 tcgcggccat aaagtgcact cactattccg ctgaataaat agacaccgcc gcatagatcc
    23881 gcaagagagg tacccacgcg cactggcgga gcatcagggt atcctgtttc catcattatc
    23941 ccgctcattg cctgaatgat ggtatcgtag gcaggagcat cttttagcgg accggtatgt
    24001 ccgaaacctg acgatgaagc atatataagg cgcgggttga tttcttgtaa cctttcccat
    24061 gaaaacccga gtttttccat tgtacctggg cgaaaattct cagctaatac atccgcttgt
    24121 ttaagcatat ttataaatat acttttatcg tgatcattct ttaaatcaag aaccacactc
    24181 tctttgccat gattaataaa actgtaatag agtgactgtc catccacata gggaccaaat
    24241 gtgcgggtat catcaccatg acccggcggc tcaactttaa ttacccttgc gcccatatta
    24301 caaagaagtt gagttccgaa aggcccatta aggacatgcg tcatatcgat aactaataag
    24361 ccttcaaacg gccctttgca ttcattattt gtcatttgct tatgccttga aaaaagccga
    24421 aggtgaaaag acacttcggc atttatgtaa aatcaataac atgattaatt atgaaaccag
    24481 tcgactgaca taaatccaca aaggagccgt gacgacaaaa cccagtacgc tcacagccaa
    24541 tgacgctgta ccagtgcggg tgtagacatt aaaccggctg gcaataatga ttccggagaa
    24601 tgccggcggt aaggcgcctg ccagtaccat catctgcaaa tgttcgctgt tcaaatgaca
    24661 tgccatacca acgagaagca gcgccagtgg catcagaatc agcttcagga aggtgttata
    24721 agcaatttcg gcactgaact cgaatttatg tgcagccaga gtcaaccccg cagcgaatac
    24781 cgctacccct gagttagcct tcgcaatcag attaaaggtt gggtcccatg ctgccggaat
    24841 ttttacccca accaacacca ggatcgttgc cagaacaggt gcccatacta ctggctcctt
    24901 tgccgcagaa attaatgcgc tcagattact attcttctta ccatccgctc ctgaggaagg
    24961 attcagcaaa tacagaccaa taggaatagt aattgcatta acaataatag aaataattgc
    25021 taccactaaa cctgttgata cggaatcacc ataaatagga tcgagaactg caaatcccag
    25081 gaatccaatg gtaggtgaac ctgcaattaa tgcacatact gctgcttctg catgagtacg
    25141 tttaaaaaat ttgtagcaac cgaaccagga gaaaaagaaa catccgacaa taaccacaag
    25201 tgatacaagg gtcagacgag tgtccgcaaa aatcatttcc ctgtttgccc gggtaataga
    25261 tacaaataga gctgcaggaa gagcgtagtt taataccagt ttattaaatg cccgggcttg
    25321 atcttctgaa aaagtttctc gtctgccgct aaaataaccc aacagcatga ttacgataat
    25381 aggcaataaa tcgccaataa aaaatgttag cataatagta tttccgataa gggtcccctc
    25441 accatcgcat aatgagttgg caagaggaga cgatgtcagt atttaattac cagcgacttg
    25501 ttttgggtta agtttggtaa tatggccact ttcagttcct gctgcagggt cgatgaccac
    25561 attaataatg gtcggtttgc gcgactgaat accggtggtt aaagcatgac gaagttcatc
    25621 tgtcgtagtg acgttatagc caacgccacg aaacgcatcc attaatttgt cataccttgc
    25681 atggtgcagc agatccgttg gtgatggtgc accagcgccg ctgagatcaa caccgtctcc
    25741 tctgtagatg ccgccattat taaaaataac gatcgtcacc ggcaggttat atcgacaaat
    25801 cgtttcaatt tccatcccac tgaaaccaaa agcactatca ccttcaatgg caacaaccgg
    25861 agaaccagag gtcacgctag caccgatggc atagcccata ccgatgccca tgacacccca
    25921 ggtgccacaa tccagacgac gacgtggttt atacatatca ataatatttc gtgcattatc
    25981 cagggtattt gccccttcat taactaaata aatatcctgg ttctcgcgca atacgtcgcg
    26041 cacagcactt aatgcattaa agtaatttaa tggttgagta tctgtgctta acttttcatg
    26101 cattttttgt gcattttgct gcttgtggat atttaaaata tcgcgccata ccagtggagt
    26161 cgtaaatgtg ttttgtttca gttctgccag cataccttgc atactggatg caatatcgcc
    26221 aacgactggc acagcaatgg ggcggttgct gtcaatttcc tgcggttcaa tatccagttg
    26281 aataaactgt gtatctgccg cccatccttt tttaccgtgt gccagtaacc aattcagtcg
    26341 tgcaccaaca agcatgacaa cgtcagcatt tgccagggca aacgaacgcg cagccgccgc
    26401 agaaagtgga tgcgtatctt cgaggatccc tttcgccata gacattggca ggaatggaat
    26461 ctgggcactt tcaataaatt cacgaagctg ttcatccgct tgtgaatacg ccgcgccttt
    26521 gccaaggata actaatggtc gttcagcttt tgctaatagc gaaattgcgc tagcgactga
    26581 cttcgggcat ggcaataatg ctggcgacgg attttcaact tttacaatcg tggttaacgc
    26641 ttcgtctttt tccatcgtcg cggccaggac atttgctggc aaatcaagat aaactccgcc
    26701 agggcgaccc gatactgaga cccggatagc gcgtgccaat gcaatgccaa gatcctgcgg
    26761 ctgattaacg cggaatgctg ctttggcata cggttttgcc gcattcattt ggtccagctc
    26821 ttcataatca ccttgctgta ggtcgacgat cgcgcggtcg ctggagccgc taatcatgat
    26881 catcggaaaa ccatttaccg ttgcgttggc caatgcggtc aaaccattga ggaatcctgg
    26941 cgcagaaact gtcaggcaga tccccggttt ttgggtaaga aaaccgcttg ccgcagcggc
    27001 atagcctgcc gactgctcat gacgaaaacc aatataacga atgccttccg cctgggcatg
    27061 gcgcgccata tccgtcacag gaatacctac aacaccataa atagtgtcaa taccattctg
    27121 ttttaatgct tcaacgatga tatgcatacc atctgtcatt tgaagttgat ctgacatgat
    27181 taataacctc gaaattttta taaattggga gccagaagcc cccgttaata tcagatggcg
    27241 tggttttgct tcattgcagc aatttcatcg tcgctataac ccagctcctg caatacagca
    27301 gcggtatgct cacctaatag cggcgcagct ttaatatccg gcgtaaaggc agagaatttc
    27361 attggacaac caacggtcag atattttcca cgcaacggtt gttccacttc gacaacacta
    27421 ccactttggc gcaaagaggg atcaagtgaa atttctttca tacttaaaac cggtgcacaa
    27481 ggaatatcaa actgagtcaa ataggccacc gcttcatgtt tatcaatagt gacagtgtat
    27541 ttttcgattt cagcaaaaat atcgaaaata tgtggctgtc gggcatgggc tgtactgtat
    27601 gccggatcgg taatccattc tggtttgccg atggctttac aggtgttttc ccagttttgc
    27661 tcctgaatag tgaaataaat ataggcgtta ggatcggttt cccagccttt acatttcagg
    27721 atccagccag gctgaccgcc accacccgca ttaccaccgc ggggaactgc atcaccaaat
    27781 gtgccattcg gatactgcgg gtattcttcc agatacccca acttatcgag acgctgctgg
    27841 tcgcgtaatt tcacgcggca aaggttcaac acggcatcct gcattgacat ggtgactcgt
    27901 tgcccacgcc ccgttttttc gcgatgcagc aaagcagcaa gtaaaccgat cagcaaatgc
    27961 attccggtgt tgctgtcacc caacgctgca gcgcttacca gcggcggacc gtcccaaaaa
    28021 cctgtagtag atgccgcgcc acccgctgcc tgagcaacgt tttcataggc ttttacattc
    28081 acataaggcg aacactcatc aaaccctttg atcgaaccaa aaatcagacg tggattgatt
    28141 tcttgaatat gctcccaggt gaagcccata tgatcaatgg cccctggatg aaagttctcg
    28201 actaagatat cagcttcgcg gatcagcttt tccattacct ctttgccttc cgctgttttg
    28261 gtatttaatt caatagaacg tttgttactg ttaagcatgg tgaagtaaag cgcatcgata
    28321 tcaggaatat ctcgcagctg gtggcgcgtt acgtcaccaa cgccgggacg ttcaatttta
    28381 atgacgtcag cgccaaacca ggccagcatc tgagtacaag atgggccaga ttgcacacca
    28441 gtgaaatcga gaactttaat tccttgaagt ggagttgaca tagcaatacc tattagttaa
    28501 ttaaatatat gttaatttag cgtttattta aggcataatg ctctctataa ataatccgca
    28561 aaatatcgtc gtagctgcac tcgatatttt taaattcctg tatcaagtgc tgataatcat
    28621 ttcatcgcca cttccaattc ctttctgcat ggtaagtagt gaggacgaaa agaagaatat
    28681 gataaatatt ggttcgcggt aatcagagag ggccgcgaca tttgtgatgt tattcttaca
    28741 actgtaagaa gcgccggtga tgtacaagta agggtatgat tacgcttttt aaagcgtacc
    28801 ataaaacata acataataaa tgatttatga tgacacggga ttattgcggt ttactttgaa
    28861 gaatataaaa tgcgagttta tagcaccgcg agcgccaaat aattttatgg ccacaacctg
    28921 tcgtattgtg gccataaaaa attgtcggct ggagggagta aaatccttta cctcaatata
    28981 ttgcctgtca gctttagttc acaaacagtg cttcgctatc gacgatgatt ccatcttccg
    29041 cacctttaag cgccaggttt gcctcgtagt attgcttttt atcaagcagt ttctgcgcat
    29101 cagcaagtgc attacgagtc tgtttgagcg gcatcagata ttggttttcc attacaccaa
    29161 caccggccag gcgaagctct tccattgcgc cctttttatc ccctttcgcc attttttcgt
    29221 tggcaatttt tatcgcggct tctttttcgg gtgtcgctac ataactctca gaaattccga
    29281 cagatgcatt aatgacaata tattggtcat cattgagatt ggtcttttta cctggcttag
    29341 cgaactttgc ccattcggta ctgtcatctg acaacaaagc tgaagcttca ttggtcagtt
    29401 cttttgcctt ttccggatcg ccatgaaaca gagccaggcg cgctacctgc acgtcacgca
    29461 tcgcatataa accttgttca gagatacgct ctgctgctgc cgttttctgg gtttgctgaa
    29521 cttgctgagt ttgctgttgt gccgctaccg gtgcattatc agcagcccat acagttgaag
    29581 atgccagaat tgctgtgacc atcgtagcca taattaaacg tttcatactt acctcattct
    29641 ttaacagttt tagaaaacgc cgccatcata ttgggtgagc atgaaatgag aaatcagaga
    29701 gcaacggtca ttttgaaagg gttatcttac agttgtagtt attatcgcaa gaagtatttt
    29761 agtcttacaa ttgtaggaat atgcttcctg tcacagagaa ctattcttat ataccacaaa
    29821 gagatgtcat ttattatatc gatgtaaata ctgattaatt aaatgtaaat acagccataa
    29881 catcgtaggt tctcagtgaa ccattttcag gataactttc tatgaaagta aacttaatga
    29941 tttttagctt atttttattg gtctctatta tggcatgcaa tgtttttgca ttttccattt
    30001 ctggtggtag aagtgagacg agctataaag agacagaaaa aacgtcagtg atgacgacaa
    30061 ctcattctac aaaacttcag ccatcacagg cgattttgtt taagttgaga gaagatgcgc
    30121 caccattaaa tctcacagaa gacattacgc cccctttttc gacaaaggcg aattatctta
    30181 ttcatcctgt gcgatagcgt ctgtcacctg gcaggtgaac aatcatgcct gatgctgatg
    30241 tgaacacctg cccaaatgaa ccatcctgac ccactagcgc ctgccatacc ccaccataaa
    30301 gcgaatcatc aagccacaag ccggggcaag aaacataaat atccccgtca ctagttcatt
    30361 accgccagtt aaccacatgg tgaagtagta aatgagtgca gcgaggatca ccatgccgaa
    30421 gaaagaagag accgccgaga ccacgttcag tacctgaccg atgtatccgc ttacgcagac
    30481 aatacacagg gcgagaaaca tccataaata aatcatagct caacctcctg ccgcgctaaa
    30541 aaacctgtaa cttcagtcac tttaatagta agacaatcct taacctctgg caatttttgc
    30601 taagcatcaa tgaaaacagc acgttaaatg caacgctatt gcgtgcggaa aattttttac
    30661 tttgcacgat taataatcag tagctgaaag cagtcagcca gtgataaagt agagccaccg
    30721 cattcatgct cctcgtggtt atgtcctgac tagtctttac actctttaca ggaaccattg
    30781 tcgtacatga tggcccaacc aattgagtgt accgctgtcg acatggaatc ttctgcgata
    30841 caacaattcg tatctacaga aggtaactat gtttccacaa tgcaaatttt cccgcgagtt
    30901 tctacatcct cgctactggc tcacatggtt tgggcttggt gtactctggc tttgggtaca
    30961 gcttccttat cctgttctct gctttctcgg cacgcgtatt ggcgcaatgg cgcgaccatt
    31021 cctgaaacgg cgtgaatcta tcgcccgtaa aaacctggaa ctttgtttcc cacagcattc
    31081 tgcggaagaa cgcgagaaga tgattgccga aaactttcgt tcactcggca tggcgctggt
    31141 agaaaccggc atggcgtggt tctggcccga cagtcgcgta cgtaaatggt ttgatgttga
    31201 agggttggat aaccttaaac gcgcacaaat gcaaaatcgc ggcgtaatgg ttgtcggcgt
    31261 ccattttatg tcgctggaac tgggcggccg cgtgatggga ctgtgccaac caatgatggc
    31321 tacctatcgt ccacataata atcagctgat ggaatgggtg cagacccgtg ggcgcatgcg
    31381 ctctaacaaa gcgatgatcg gcagaaataa tctgcgcggc attgtcggtg cactgaagaa
    31441 aggtgaagcg gtatggtttg ctcccgatca ggattatggt cgtaaaggca gctccttcgc
    31501 gccgttcttt gcggtggaaa atgtcgccac aaccaatggt acgtatgtcc tttcacgtct
    31561 ctctggcgca gccatgttaa ccgtaacgat ggtaagaaaa gcagattaca gcggatatcg
    31621 tttgtacatc accccagaga tggaaggcta cccggcagat gaaaatcaag ccgctgccta
    31681 tatgaacaag attatcgaaa aagagatcat gcgcgcaccg gagcagtacc tctggatcca
    31741 ccgtcgcttt aaaacgcgcc cggtgggaga atcgtcgcta tacatttaac aaactgctct
    31801 tactctttcc ctcctcaaat cagccataat aacctcatgg tttttatggc ttttttctta
    31861 aggttttttc cttcgctgaa tctctaatta ttaccgtcag aaattaaact gtcgccgata
    31921 tgagacacct gtaagttact aaaagtaaat aagaaaatgc ctcttgtctc gtttcagttt
    31981 taggcgtaca ttagcgccgt ctggagcagt ttagctcaga attctggggt gatgcccacg
    32041 gtaaaataca ggacttttaa ggtcgttttg cccggtgtca actcactgta tcaggtgtaa
    32101 tgaagtcatt caggcgtaac agtaattacg cggagagatg taaagtgaaa tatttcttta
    32161 tgggcatttc ttttatggtc atcgtttggg ccggtacttt tgccctgatg atctaaaagc
    32221 aaactgtgca atgaaaaaca ggagccatct ggctcctgtt tgcatttctg gcgatggtgt
    32281 ttattccgcg ttttcgtgaa tatgtttgct gctggcgggt agtaaaccgt cggcacggaa
    32341 catcgcttta attccacgaa tcgcctgacg aatacggtcg cggttttcaa tcagtgcaaa
    32401 gcgaacatgc gtgtcgccgt agtcgccaaa gccaatcccc ggcgagacac agaccttcgc
    32461 ttcgttcagc agcttcttgg caaattccag cgagcccatg gcagcatacg gttccgggat
    32521 tttcgcccag acatacatcg aagccttcgg catttcgacc atccagcccg cttcatgcag
    32581 cccttttacc agtacatcac ggcggcgttt gtactgttca gcaatgtcgc gcacgcactg
    32641 ttgatcgccc tccagcgccg caatcgctgc cacctgcaac ggcgtaaagg tgccgtaatc
    32701 gtgatagctt ttaatacgtg ccagcgcgct gaccagcgtt ttgttgccaa ccataaagcc
    32761 gatacgccag cccgccatgt tgtagctttt cgacagcgta aagaactcga ccgccacatc
    32821 gcgtgcacct ggcacctgca tgattgacgg cgctttccag ccatcgtaga cgatatcggc
    32881 ataggccagg tcatggacca ccagcacatc gtagcgtttc gccagcgcca caaccttttc
    32941 gaagaactcc agctccacgc attgcgcggt tggattcgac gggaagccga ggatcatcat
    33001 cttcggtttc ggataacttt cgcgaatggc gcgttccagt tcgttgaaga aatcgacacc
    33061 ttccaccagc ggcactgagc gtacctgcgc cccggcaatc accgcgccgt aaatatgaat
    33121 cgggtagctt ggattaggca ccagtacagt gtcaccatga tccagcgtcg ccagcatcag
    33181 atgcgccagt ccctctttcg aaccaatagt gacgatggct tctgattccg ggtcgatctc
    33241 aatgccgtag cgatcctgat accagcggga aatggcgcga cgtaaccgcg gaatgccgcg
    33301 tgacgtggag taaccatgcg tgtccgggcg ctgggccacc gtacataatt tttcgacgat
    33361 atgcggcgga gtcgcaccgt ccgggttacc catactgaaa tcaataatat cttcgccgcg
    33421 ccgacgcgca gccattttca gttcagcggt aatgttaaaa acatagggcg ggagacgatc
    33481 aatgcgcgta aagcgacgtt cagggcgagt gtcagccata atttcctcag attaacgtta
    33541 gcgcccggac cgtccgagcg acgctgccac gatggtggct gttttgaaaa tagcctgatt
    33601 aatttctgtc tgtccagcgt tttagaacat ttttattaat gacgttaaat gaggaacttt
    33661 gctttaaaaa caggaaattt atctgcgcta acaagaaaat taccacccat tttattaaca
    33721 taataatttc aataaattta ctaaccagaa gtgcattatc aagagatgca ttgctaatac
    33781 accatttatc tatcaatccc cttttaaaat tgtggttttt cctcaaaaac ggcctgctgg
    33841 tcatcagcct tcaggttacc tatcatagag gtttaatcct tattcagagt cacccgtgca
    33901 cgaaatattc aacatgctgc tggcggtctt cgatcgggca gcgttaatgc ttatctgcct
    33961 gttctttctc atccgtatcc gtctgtttcg cgaactgttg cacaagtcgg cgcactcccc
    34021 aaaagagttg ctcgccgtta ccgccatttt ctcgctgttc gccctgttca gcacctggtc
    34081 cggcgttcct gtagaaggtt cgctggtgaa cgtacgtatt atcgcggtga tgtcaggcgg
    34141 gattctgttt ggcccgtggg tgggcatcat taccggcgtg attgcgggta ttcaccgtta
    34201 tttaattgat atcggtggcg tgacggcgat cccctgcttt atcaccagca ttctggcggg
    34261 ttgtatatcg ggctggatca acctgaaaat ccccaaagca cagcgctggc gcgtcggtat
    34321 tctcggcggc atgttgtgtg aaacgttgac catgattctg gtgattgtct gggcaccaac
    34381 taccgcattg gggatcgata tcgtctctaa aatcgggatt ccaatgatcc tcggcagcgt
    34441 ctgtatcggc tttattgtgt tgctggtaca aagtgttgag ggcgaaaaag aggccagcgc
    34501 cgcgcggcag gccaagctgg cgctggatat cgccaacaaa acgctgccgc tgtttcgcca
    34561 tgtcaatagc gagtcattac gcaaggtctg cgaaattatc cgcgatgaca ttcacgctga
    34621 cgcggtggca atcaccaata ccgatcatgt gctggcctac gttggcgtgg gtgaacataa
    34681 ctatcagaat ggcgatgact tcattagccc gactacccgc caggcgatga attatggaaa
    34741 aatcatcatt aaaaacaatg atgaagccca ccgcacacca gagattcatt ccatgctggt
    34801 gatcccattg tgggagaaag gcgttgtgac cggaacgctg aaaatttact actgccacgc
    34861 gcatcagata acctcgtcat tacaggaaat ggcggtcggt ctgtcacaaa tcatctccac
    34921 gcaactggaa gtttcgcgcg ctgagcagct gcgtgaaatg gcaaataagg cagagcttcg
    34981 cgccctgcaa agcaaaatta atccccattt cctgtttaac gctttgaacg ctatttcatc
    35041 gtcaatccgt ctgaatccgg ataccgctcg ccagctgatc tttaatctgt cgcgttatct
    35101 gcgctataac attgaattaa aagacgatga gcaaatcgat atcaaaaaag agctgtatca
    35161 aattaaagac tatattgcca ttgagcaggc ccgctttggc gacaagctga cggttatcta
    35221 tgatattgat gaagaggtga attgctgcat tcccagcctg ctgatccagc cgttggtgga
    35281 aaacgccatt gtccacggta tccagccttg caaaggtaaa ggcgttgtca ccatcagcgt
    35341 tgcagagtgc ggtaatcggg tacgcattgc ggtgcgggat accgggcacg gcatcgatcc
    35401 aaaggtgatt gagcgggtcg aagcgaacga aatgccgggc aataaaattg gcctgctgaa
    35461 tgtccatcat cgcgtgaagt tattgtatgg cgaggggctg catatccgcc gcctggaacc
    35521 aggaacggaa attgcgtttt acattcctaa ccaacgcacc cctgtcgcct cacaggctac
    35581 gttattgctt tgagccggag tgatattgtg aaagtcatca ttgttgaaga cgaattcctg
    35641 gcacaacagg aactgagctg gctaattaaa gagcacagcc agatggagat tgtcggcacc
    35701 tttgacgacg gtctggacgt gttgaagttt ttgcagcata accgcgtcga cgccattttt
    35761 ctggatatca atattccgtc gctggatggt gtgttactgg cacaaaacat cagccagttc
    35821 gcccataaac cgtttattgt gtttatcacc gcgtggaaag aacatgcggt agaagcgttt
    35881 gaactggagg cgtttgacta cattctcaaa ccgtatcagg agtcacgcat taccgggatg
    35941 ctgcaaaaac tggaagcagc ctggcagcaa cagcagacca gcagtacgcc tgccgcgacg
    36001 gtaacgcgtg agaatgacac cattaatctg gtgaaagatg agcgaatcat cgtcacgcca
    36061 attaacgata tctattacgc cgaagcgcac gagaaaatga cctttgtcta tacgcggcgt
    36121 gaatcctacg taatgccgat gaacattacc gaattttgca gcaaactgcc gccatcgcat
    36181 tttttccgct gccatcgctc attttgtgtc aatctgaaca aaatccgcga gatcgaaccg
    36241 tggtttaata acacctatat tctgcggctg aaagatctgg attttgaagt gccggtcagc
    36301 cgcagcaaag ttaaagaatt ccgccagtta atgcatcttt aatcgggaac tttcatgaaa
    36361 gcaccaggct tgcccgccga tcaacaattt ttcgccgatc tgttcagcgg cctggtgctt
    36421 aacccgcaac tactggggcg cgtctggttt gccagccagc ctgcctcgtt accggtgggc
    36481 agtttatgta ttgattttcc ccgtctggat atcgtgctgc gcggcgaata cggcaatctg
    36541 ctggaagcaa agcagcaacg tatggttgaa ggagaaatgc tgtttattcc ggcgcgcgcg
    36601 gctaatttac cgatcaacaa caaaccggtg atgctgttaa gcctggtgtt cgcaccgacc
    36661 tggcttgggt tatcgtttta cgatagccgc accacgtcgt tgttgcatcc ggttcgccag
    36721 atccaacttc ccagcctgca acgcggcgaa ggtgaagcaa tgcttactgc cctcacccat
    36781 cttagccgtt cgccgctgga gcagaatatc attcagccgc tggtgttaag tttgctgcat
    36841 ctttgccgta acgtggtgaa tatgccgccg ggcaattcgc agccgcgcgg cgattttctc
    36901 tatcacagca tttgtaactg ggttcaggat aattatgccc agccgctcac ccgtgagagc
    36961 gtggcgcagt tttttaatat cacgccaaat catctgtcaa aactgtttgc tcagcatgga
    37021 acaatgcgtt ttatcgagta tgttcgctgg gtacgaatgg caaaagcgag gatgattttg
    37081 caaaaatatc atctgtcgat tcatgaagtg gcgcagcgtt gcgggtttcc ggatagcgac
    37141 tatttttgtc gcgttttccg gcgtcagttt ggtctgacgc cgggagagta cagcgcccgt
    37201 tttcagggct aacgtcagaa ggttaattct gtttccagca gcgtcaggat gctttgtgca
    37261 tcttgcgcgg caaacaacga ctggcggaag tttttattca ccagtttacg cgccagctgc
    37321 gagaagactt tcacatgatt catcccttcg ttagcgccca gtgtcagcat gatcaccagt
    37381 tcgacttcgc ccatttctga ctgccagtca accggtttca ccagtcgggc aatgctgata
    37441 ctggaatgac ggatccactg agatttggtg tgcgggatcg ctacgccaaa accaacgccg
    37501 gtggtaacaa tctcttcccg ctgccagaca tcctcttcca gctcgaacgg atgttcagtg
    37561 cgcccgttaa cgccgaggtt accgcacagg aactggatcg cctgctcttt attgctaaaa
    37621 gcctgatcaa caaagatatt ctccagcgcc agcagagggc gaacgtcttc ttccggcgta
    37681 aacgcggtga gtaacgcttc aatctcctgc gcgctgcggc attcacatgc ctgacgcgcc
    37741 agttcccgac acgcctcgct atccagttga cgaagctggc ttttcaccgc cggaatgcgc
    37801 gggctactca tactcagctc gtccaggccc agcccaagca gtagcggcag ataacggctt
    37861 tcaccgccca gttcaccgca aatacctacc catttacccc gctgatgcgc agtggtaaca
    37921 atctgctgca acatgcgcag gaacgatggc gtaatcgggt tatacagcgg cgatacgcgc
    37981 gggttattac gatcgaccgc atacagatac tgggtcatat cgttggagcc gatactgaag
    38041 aaatcgacct catcgcagaa gtgatcgatg atgtagcaca ccgacggcac ttccaccatg
    38101 atacccagcg taatcgtctc tgcatgacgc aggccatcgc gcttaagctc aacgatcgct
    38161 ttttggatct cgcccttcac ccataagatc tgatcgaggc tgtgaaccat tgggatcatc
    38221 aactgggcgt tgccgaaact ggcggcgcgc aaaatggccc gcagttgagt gcggaacagg
    38281 ccagcaaatt ccgggtaaat tcgtaccgca cgatagccga ggaacgggtt ctcttcctgg
    38341 ggaatgttga ggtaaggaat gcttttatcg ccgccgatgt ccattgtgcg gaagataatc
    38401 ggcttgtcgc ccgcggccag tagcacctgc tggtaggctt caaattgctc ctgctcgtcc
    38461 ggcgcactgt cgcgatccat atagagcatt tcggtacgaa acagcccgac gccttccgcg
    38521 ccgttggcaa acgcgccagg cgcttccagc gcggtgccaa tattcgccgc gatatcaatg
    38581 cgcttgttat cacgggaata ggccagctgc gcggcggcct gcgcctgctg ctgctgacgt
    38641 ttatccgcca gcatctgcgc aacctgataa taaccgctca ccgcgtcatt cgggttaatc
    38701 gccaacacgc cgcactgggc gtcaagcacc gccggttgcc cggcatagcg cgcaatggca
    38761 tccagcggca ggccgctcag taccgggatc gccgaggcgc gggccagaat cagggtatgc
    38821 gaggtgcgcc cggttttctc cagaatcatg cccgcaagat tttttaaatt gaggctcaaa
    38881 aactggctag gggttaaatc ctctgccacc agaatggtcg gtttttccag caccagattg
    38941 ttgcgcggtt ttagttccgg ccaggtgata tgcagtaact gttcgctgat atcgcgaatg
    39001 tcgctaaccc gctcgcgcag ataatcactg gcagaagcag aaagtttggc gcaaacctgc
    39061 tccatattgc tgatgatcgc cgcccccagc ccctgatgct gttctgccat caggtgacgg
    39121 atattgcctg caaattcatc atcctgaatc agcgacaaat gggcgctgag gatagttttg
    39181 ctttcgccgt cacgctcacg caattgctgg ttcagttgct cggcaagcgt tgccaggctg
    39241 tgctccagcc gggtggaatc ttgcgcactg gcggggatcg cccggtaact gtcgaggctg
    39301 tcgctctgta acagggtcag cgtacccacg ccgacgccgc ttgccagcac attgccgtac
    39361 agtaaatcag gattcagacg gcttaatgaa cgcggcagcg gatgcgccgt cagttctgcc
    39421 tgcgttggct ggacgctgtc gctgtcgata aagcgcacct ggatgtactc ttccagcacg
    39481 cgccgcgcct gctcttcatc gctgccgcta atgttcaggc tgcaaccgtc attaaatagg
    39541 gtgccggtgc caatcagcgc cagcgagctt ttggcatctg cctttgcgtt ctggcgatgg
    39601 ttaataaaag tgatttcgct ttgccactgg ctgcactgtt ctttaagttc ccacgccgga
    39661 cgagcatgca gaccgtttgg caaaggacag agaaattgaa tcgttaacat aaccgctccg
    39721 ggttaatcag gagatcatct gaaatccgtc agttgaacaa ccgtctcacg cgtaagacgt
    39781 tgaataaggg cagataaaag ctgctgcatc tgcaaaatgt cacggcaatc ggcaatcgat
    39841 gcggcgcaat gtccgtggcg ggtggcaggc cccatcacca ctgtgggtac gccagtgccg
    39901 gttaagtgca ccgccccacc gtccgtgcca ccgttgctga acatatccgc ctgcaacggc
    39961 acgccaattt ctgccgccac ggtttcaatc cacgcggtaa gttttggcgg cgcaatcagc
    40021 gatttatcgc ttaacaccag catcggcccg ttaccaatct gacgatggtt agccgcgcca
    40081 taatcaaagt ttttcgccca gcaggcggta tcaagcacaa tggcgacgtc cggcgacacc
    40141 gcgcgggtgg cagtttgccc gccgcgtaat cccacctctt cgctggaact tgccaccagc
    40201 cacacctccg caggcagttc ggcgtcgtgc agctcacgta gtaacgtcac cagcagatag
    40261 caaccgaggc gatcatcaaa ggctttcccc atcacccgct ggtgagggag aacctgaaaa
    40321 gttgtatcaa aggtgacgcg atcgcctgga cgaattcccg cctgcatcac ttcgtcatac
    40381 gagcgcgcac caatgtccac gcgtatggtg ctgacgtcat tcccctgccg gtcgccgtca
    40441 agcaggcctg gaattttgca ctcttcacgg gtagtgatac gcaccgactg aagctggcgg
    40501 gcagccatgc gtacattgcc aaccggcaac acatcgatcg ccccttcgcg ggagatgctg
    40561 cgcaccataa atcccacttc gtccatatgc gcacagatca tcaccttcgg acctgtcgac
    40621 tcattgagtc ggatcagcac cgatcccagc ccatcaaatc gcacttcttt ttgcaggcga
    40681 tccgcttctt ccagcaggat ctgccgtact tcctgttccg aggaggcgat cgcatctgcc
    40741 tcgctcaacg cttttaataa cgataaatcc attatgcctc tcccgtgagc aacactgttt
    40801 tcggcatggc gtagagcact tctacgcctt gcggggtgac cagcacaaca tcttcgatgc
    40861 gcacgccccc ttgccctggc aaataaatcc ccggctccac ggtcagtaac atgcctggct
    40921 gtagcgtcgt ggtgtcccgc ggtgaaaaac gcggatcctc atggacttca ataccgatag
    40981 catgaccggt gttatgaccg aaatagtcgc cataaccggc ttctgtaatc acccggcgcg
    41041 cggcgtcgtc aacctgctgg cagcgtacgc cggggcgaat cgcggagatt gctgcgagct
    41101 gtgcctgcag gacaatctga tagacgttaa acagcgggtg ggattcggca cttacccctt
    41161 cgccattcac cagcaaggtg cgcgtcatat cagagcagta gccctgatac agcgcaccga
    41221 aatcgagagt gacaaactcg cccgctgcaa caatcttgtc gctggctttg ccgtgcggca
    41281 gcgccccacg ccagccgctg gcgacaatgg tatcgaaaga ggctttttct gcgccctgct
    41341 ggcgcataaa ccactccagt tcagcggcta tctcgcgctc gctcatcccc gcctgaataa
    41401 agcggcgaat atgctctgca ccgcgatcgg caatcccaca ggcgagacgg attttctcca
    41461 cctcctctgg cgttttgagt tgccgcagca catccggcgt ggcgctaacc agtttcgcat
    41521 tgagttcaga ctgccagcgg tgcgcggttt cccaactcac ctgctggcct tcaaaaccga
    41581 gcgtttgcaa ctgctcatcg gcaaagattt gcctgacgat ggtggcaagc gtgtgcatcg
    41641 cgtcgagcaa atgcagctgg taaccttgcg tgcgggcttc tacatccgcg taatagcgcg
    41701 agtccaccag aatgtgcgca ctttcacggc aaatcaccac atagcctgat ccggtggaga
    41761 tccccagatg cggctgtttg ttctgccgtg aggagagaag caccgcatcc agttgttgcg
    41821 ccttaagcca gtcgcgcagc gaagcgagta atgtcatcag gattgtcctt cttacaggct
    41881 atcgattaac aatttgcctt tacggaacat catcagacgc aggaacacca ccatcagcgc
    41941 agttatgact gcccccagcg cgatccccgc catgtagacg ccgaggttag tgaccagcgg
    42001 ccatgcccag atagcggact ccgggaacca ttgcactgcg cccagccaga cagcggcggt
    42061 tgagccgaca atcgcgccga ccatatagga cggaatggcg gtgatggggc tttccagcgc
    42121 aaacggaatc gcaccctcac tgatccccat aaacgccagg aacatggcgg ttttaccctg
    42181 cggataaagc tgagcactga acagacgttt gccggttaaa cggcggtcga taatggtcgc
    42241 cagacccaga ccaatcggcg gaataacaat agcgatggaa cgcgcggtaa ccggtaatac
    42301 gtggtcagtg gtaaagctga aggcaacgaa accagctgct ttgttgatcg ggccaccaag
    42361 gtcgattgct gtcgccgcgg cgatccccat cgcgtacatt agcgcaccct tctcacctgc
    42421 ggcagtcagc acggtacgga taccgccgtt gatccagcca ccaaaggggg tgatgacgta
    42481 gtacatcgcc agcatgacaa aaatggcgga aagaatcggt agcaaaaatg tggttttgaa
    42541 cgctaacagg aaatccggta actggatttt ctggttcatc cacttcacaa ggtaaccagc
    42601 aacgatagaa ataatcaggg caccaatgaa ggtggacggc accggcgaag aggtcgccca
    42661 ttgcatcgtg ctgggatcga agttcagcag ttgcgtcggc tgggtagaca tcaacccacc
    42721 gataaaacca gccgggaacg ccagtttgcc accgatagag ttcgccacaa atgcagcaaa
    42781 catcggaatg gcaaaaccaa acaacacgcc gccgaaggac tgcgacaacc aggcaaattt
    42841 cagtaaggag aggtcgaaac cggagaattt cccgctatta agagcatcca tgatgccgat
    42901 atcagcagga attttaagcc agctataagc aatcagctgg ctgaaagcga ggatcacacc
    42961 gcccataatt aaggtcggca ccatgcgtga aatccctgac atcacatgct gcgggagttc
    43021 accccagaag ctggttttag aggcctgcgg attcttaact gccgctgccg caccggatgc
    43081 gccaggcaca acggttgcac tgcgtttttt aatggccata acaattatcc ctgtcgaata
    43141 ttactgttgt tcagaggcaa tcatctcttc gatttctttg atgatgcccg cagcgttttt
    43201 aattgcgtcc tgcaaagtga tttcataaac gtcgcgtgat tcgaaacgtt cgttatcttc
    43261 cggggtaacc gccacggagt ggatgatgat ggtcgcttcg gcgatatcct gcgcggtcag
    43321 gcgattctgg atgccgtccg cgccctgagt ttcaattttc acttcatagc ctgcttccac
    43381 cgccgcttct tccagcgcct gggctgccat aaaggtgtga gccagaccca tcgggcaggc
    43441 acataaagca atcagtttct tactcatcgt taattcctcg ttcagtgata gttgacgagt
    43501 gaaattatga acgtgcaaaa caaatcgccg ttaccagaca aataatgcat ttactggaga
    43561 attaatcccg ctgtacggaa gtgtgacgca ggtcggcctt gtgaggggac acaaaggaaa
    43621 attgaattaa ctgtatgatt taaaagatta tcgggagagt tacctcccga tataacagga
    43681 aggatttaga gaatgtgacc taaggtctgg cgtaaatgtg caccggaacc aagaaggccc
    43741 ggattgtcat ggacgatgag atacaccgga atatcatgga catattcttt aaagcgccct
    43801 ttatcttcaa atgcggcacg gaaaccagag gctttgaaga actcaaggaa gcgcggcacg
    43861 ataccacccg caataaacac gccgccaaat gtcccgagat tgagcgccag attgccgcca
    43921 aaacggccca taatgacgca aaacagcgac aacgcgcggc ggcaatcggt gcagctgtca
    43981 gccagcgcgc gttcggtaat atcttttggc ttgagatttt ctggcaggcg gttgtcggct
    44041 ttaacaattg cgcgatacaa attcaccagc ccagggccag aaagcacgcg ctctgccgaa
    44101 acatgaccaa tttctgcacg cagtatttcg aggataatgc cctcttcttc actattcggc
    44161 gcaaaatcaa cgtgaccgcc ttcgcctggc aagcttaccc aacgcttatc gacatggacc
    44221 agatgcgcaa cccctagccc cgttccggca ccgtaaaccg caataggctt accttctacc
    44281 ggttctgcgc caccaaactg aatcagatgc tcttttttca gcatcgggat cgccatcgat
    44341 acagcggtaa aatcgttgat aatttccaga tggctaaaac cgagattctt tttcatttcg
    44401 gcaattgaga acgcccaggt atggttggtc attgccaccc agtcaccggt aattgggcaa
    44461 gcgatggcaa tacagccgtc ttgcacctcg atcttatgtt cttcaagata aacgcgaatg
    44521 accgcttcga ggctggggta atcaagccct gaataggtct tagcctgcga gatttcacca
    44581 ctggcaatat cacacagagc aagacgtgcg ttggtgccgc ccacatcacc gactaatgca
    44641 tactttgtca ttcttcaact gctccgctaa agtcaaaata attctttctc acactgtaaa
    44701 tacctggggg cataacaaca acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt
    44761 cacacaatta ctttatcgtt tcagcaccaa ttgcagcgat gcctttttgc aagctgggca
    44821 aactaagtat ctgaccccgc ataaggaata gaacatgctc catccgcgag ccagaaccat
    44881 gttgttatta tcgctccccg ccgtggcaat tgggattgcg tccagtctta ttctgattgt
    44941 ggtgatgaaa atcgcctcgg tattacagaa tttgctctgg caacgactgc cgggaactct
    45001 ggggatagcc caggattcac ctctctggat catcggtgta ttaacgctaa cgggtattgc
    45061 agtggggttg gttatccgtt tcagccaggg tcatgccgga ccagaccccg cctgtgaacc
    45121 gctgatcggc gcaccagttc cgccctctgc gctaccagga cttatcgtag cattaattct
    45181 tggtcttgct ggtggcgtca gcctggggcc agaacatccg atcatgaccg tcaatatcgc
    45241 ccttgcggtt gccattggcg ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat
    45301 tttagcctct gccggaacca tcggcgcgct gtttggcaca cctgttgcgg cggcgttgat
    45361 attttcgcaa accttaaatg gcagtagtga agttccgcta tgggatcgtc tttttgcgcc
    45421 gttaatggcg gcagcagctg gtgcactaac taccggatta tttttccatc ctcatttttc
    45481 actgcccatt gcgcattacg gacagatgca aatgaccgat attctcagtg gtgcaattgt
    45541 cgcggcgatt gccatcgcag cagggatggt cgccgtatgg tgcttaccac gattgcacgc
    45601 gatgatgcat caaatgaaaa atccggtgct cgtgctgggt attggcggat ttattctcgg
    45661 tattctgggg gttatgggtg gaccagtttc gctgtttaaa gggctggatg agatgcagca
    45721 gatggtggca aatcaggctt tcagcaccag cgattacttt ttgctggcgg tgattaaact
    45781 tgccgccctg gtcgttgctg ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt
    45841 gtttgtcggc gtggcattag ggttgatgct gcatgagcac gtccccgccg ttccagcggc
    45901 aataaccgtt tcctgcgcta ttctcggcat cgtgctggtg gtaacacgcg atggctggtt
    45961 aagtcttttt atggcggcag tcgttgtacc caataccaca ttgctaccgc tgctctgtat
    46021 cgtcatgctt ccggcatggc tgttattagc aggtaagccg atgatgatgg tcaatcgtcc
    46081 gaagcaacag cccccccacg ataacgttta gcaaaaaatg ctctccttta tgatttaaga
    46141 gttatggcga tgatacgggg ctttacctcc ccgtaatatt gcgttaacag gccgctgacg
    46201 gcttcgatcg tgaaggagaa taacaatgtt caggtcactg tttctggcgg ccgccctgat
    46261 ggcatttacc ccgcttgcgg caaacgcagg tgaaatcacc ctactgccat caatcaaatt
    46321 acaaattggc gatcgcgatc attacggtaa ttactgggac ggtggtcact ggcgcgaccg
    46381 tgactactgg catcgcaatt atgaatggcg taaaaaccgc tggtggcgtc atgataatgg
    46441 ctaccaccgt ggctgggata agcgtaaagc gtatgagcgt ggctatcgtg aaggctggcg
    46501 cgatcgtgac gatcatcgcg gaaaaggccg cggacatgga caccgccatt aaacacattc
    46561 gccaatggag cacaatgcct gatgcgacgc tagtgcgtct tatcaggcct acaaaccggc
    46621 tctttaacgc gtagggcgga taaggcgttt acgccgcatc cgccaatagt gccagatgcg
    46681 acgctcattc aactacaatc ccagcgccgt ccccaccaac aaccagatat tcagcgccac
    46741 gaccagcact acaatcaccc agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc
    46801 catcaacttg ctgtcactgg tgaaaatcag cagtggaacc agcgccagag cgataccaaa
    46861 acttaacagc acctgactca taaccagaat ccgtgtcgga tctaatccca tcagaatgac
    46921 aataaatgac ggcaacatgg taacggtgcg acgcacccac agcgggatat ggaagcgaat
    46981 gaagccctgc atcaccacct gccccgccag tgtccccacc accgttgatg acagtccggc
    47041 agcaaccagg cttaatccaa agaccgttgc cgcagcatgg cttaacaacg gttgcagcgt
    47101 caggtaagcc tcatcaagat cggcaacgcc agtatgaccg gaaaagtgga acgccgccgc
    47161 agccgtagcc atcatcgcca gattgacaaa accggcaata gtcatagcga tagccacatc
    47221 ccatttggtg gcagaataac gttgttgacg cgaaccacca tgtaaatgct gagtcagcga
    47281 agagtgtaaa taaatcacgt gtggcataat cgtcgccccc aacacacctg ccgccaggaa
    47341 gactgcctcc gaggtgggta aactcgggat taccattcct ttacccagtt gcgccaggtt
    47401 aggctgggag aaaatcaact cgacaatgta agccgcagca acaaacagca gtaacccgcc
    47461 aatcactttc tccagcggtt tttgccctcg acgttgcagc attaaaatca ggaaagtcgc
    47521 gatccccgtc agcaccgcgc cctgcaacag cgaaacaccc agaatgagtt taaaaccgat
    47581 cgccgcacca ataaattccg ccaggtcggt tgccatcgca atgatttctg cctgaaccca
    47641 atagaaccac actacgggac gcggatagtg atcgcgaatc tgctccgcca ggtttttacc
    47701 ggtggcaatc cccagtttgg cagacagtat ctgaatcagc atcgccatca ggttggccca
    47761 aacgacaacc cacagtagct gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa
    47821 gttaccagga tcgatataac caatcgccgc aatgaacgca ggtcccatta atgcgagcct
    47881 catcttgcgc gccgcccgtc cgctgctact ctcaacgcga tagttagtca tcttgtgcct
    47941 ctaaaacata gcctttgcta tgtttcatgc tatgtcaaac gagaatgatt atcaaattca
    48001 tttaagtgga ttgtgatgat ttctttgata gaccagaatt atgacgacga aaagattgat
    48061 gacttgaatg ttgtgattat gtttaatgtt agcacattta cataactttc agcttccata
    48121 cacaacatag cagaaatgta tgacagctca ctatttttga agcttgtcac aggacgtcat
    48181 tatagtgtgt gtcagatctc gttttcctta accatgttac atagaatgtg cacggaaatt
    48241 taacctgcct catatttgga gcaaatatgg accgcgtcct tcattttgta ctggcacttg
    48301 ccgttgttgc gattctcgca ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt
    48361 atgttattca actgcttgtt atcgaagtgt tactggcgtg gttcttcctg aactccgacg
    48421 ttggtttggg cttcgtgaaa ggcttctccg aaatgtttga gaaactgctc ggatttgcca
    48481 acgaagggac taacttcgtc tttggtagca tgaatgatca aggcctggca ttcttcttcc
    48541 tgaaagtgct gtgcccaatc gtctttatct ctgcgctgat cggtattctc cagcacattc
    48601 gcgtgttgcc ggtgattatc cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg
    48661 gcaaactgga atcctttaac gccgtcagct ccctgattct gggtcagtct gaaaacttta
    48721 ttgcctataa agatatcctt ggcaaaatct cccgtaaccg tatgtacacc atggcagcaa
    48781 cggcgatgtc caccgtgtcg atgtccatcg ttggtgcata catgaccatg ctggaaccga
    48841 aatacgtcgt tgcagcgctg gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga
    48901 tcaaccctta ccgtgttgat gccagtgaag aaaacattca gatgtccaac ctgcacgaag
    48961 gtcagagctt cttcgaaatg ctgggtgaat acattctggc aggtttcaaa gttgccatta
    49021 tcgttgccgc aatgctgatc ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg
    49081 ctaccgtgac tggctggttt ggctacagca tctccttcca gggcatcctg ggctacatct
    49141 tctatccgat tgcatgggtg atgggtgttc cttccagtga agcactgcaa gtgggcagta
    49201 tcatggcgac caaactggtt tccaacgagt tcgttgcgat gatggatctg cagaaaattg
    49261 cttccacgct ctctccgcgt gctgaaggca tcatctctgt gttcctggtt tccttcgcta
    49321 acttctcatc aatcgggatt atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta
    49381 acgtggtttc tcgcttcggt ctgaaactgg tttacggctc taccctggtg agtgtgctgt
    49441 ctgcgtcaat cgcagcactg gtgctgtaag accatacata aaaaagccgg ggataattcc
    49501 ccggtttttt acgcctgtta atcagctaat ggctgcgggc gaccaatcaa atacccttgc
    49561 agatattgca ccccgagctt atgcaatagc gcctgctgct gttgcgtctc gacaaactcc
    49621 gcgaccacac tcaatgactt cgctttcgcc agatcggtaa ttgatctcac aatcatcgca
    49681 tccagcgtgt tcgtgacgat atctttcaca aagacgccat caattttgat gatatcagcc
    49741 tgcaaacgct ttaaccgttc gtagttggca tatccggtgc caaagtcatc aatcgcaatc
    49801 cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc tgctttctgc gttagaaaac
    49861 gcctgctcct cggtgatctc aagaatgacc gcctgcgggg agatgtgata acgcttaaac
    49921 agacgaataa tccgcccggc aatattcttt tgcagcagcg tgagcggcat taaattgact
    49981 gaaaagcgcg ggcctttttt gtcgcaagga tgtgttgcca gccacttcaa cagggattcc
    50041 agcacttgca aatcaaaacg cgcgctaagg ttaaactgag caataagggg cagaaactta
    50101 tccggggtca taatgccgcc gtcatatttc agtcgcgcga ggatctcatc ataaccttca
    50161 ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat caccctgatc taacgccgta
    50221 cgaatggtat tcagcaatag cacctgttta gtggtctgcc cggaaaccat ctcctctctg
    50281 ctatccagcg ccagcacatg gtgatgtgcg caggattgct ccgccagcca gcttaactgc
    50341 cccaacaagg gttgcagggt ttcctgattt ccatcaaaac gcccccaggc agcaccatag
    50401 cccatatcca gcccggtatt gttccagtga atttgccgac tattcaggat gttaaccata
    50461 tgctggagtc gcccttccgt ttccggtcca ctcagcacta acagcagttc actacccggc
    50521 aattgataca acttttcgtt ttcctgcatc aacggcagca gcgtacggta aattgatcgg
    50581 atacagtgaa cgcgcatcat taagccgtaa tgacgactca taaactcaag attatcaatg
    50641 cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgttctaa cgcacgaaag
    50701 ttgggtagga gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg attaagccat
    50761 tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga aatcagcacc
    50821 gcgagaataa atgccagcga atattcggtt agtacccctt gcaaaaagtt ctggttgtaa
    50881 ttcagcaggc aaagcgttga aacagcccag gtgagattta aaaacggata gcgaagctta
    50941 ccgacaccga gggtaaagat gataaagaaa acaggtacca gataaccagc aataaagtca
    51001 ttttcatacg gtgtgcacat cagaagtagc agcacgctaa gagctgccag ccagcttaag
    51061 gtaaatgcgc gtttctcttt gctcaacgac ggagcgatat ccctacgcca caatatctgt
    51121 gcaaagtggg gacttacaat catgcgggcg agatagtaaa aaagcatgtt gtaaatcagc
    51181 actgcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa
    51241 aaggtagata tctttagcgg aaaatcaaag aaatttccca caagatacat gctgcatttg
    51301 atgccaatcg gcgccaccaa accaagccag aaaagacgtt gccagacata tcggctggtc
    51361 aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc acacaacacc
    51421 acaaacgtct ggctgccgag catgaccgcg gtctgtaaaa aagagagatg aaagttccac
    51481 agattggtgc aaaacatccc gcataaaacg gggacgacac cgcgccagcc aaagataaac
    51541 aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact ggagtggata
    51601 atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag cgtaaacgct
    51661 agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag tacactactc
    51721 tcactgatcg gggttttcag gcacacaaat aataagttta ttcgcatcgc gaagcaaaca
    51781 ggggccagcc tgttgccgat ttttcatcca atgatgcttt caccaggcgg cgctaacccg
    51841 gacgtagact gtattacaaa agcggcaaaa agcagagaca aaaaaccccc gctttacagc
    51901 gagggtttga aatttggtgg agctaagcgg gatcgaaccg ctgacctctt gcatgccatg
    51961 caagcgctct cccagctgag ctatagcccc acgatgcgtt tacgtaccaa atttgctggg
    52021 tgcaaaattt ggtggagcta agcgggatcg aaccgctgac ctcttgcatg ccatgcaagc
    52081 gctctcccag ctgagctata gccccatcac gtaaagcttg tcgagttgac gggcggcatc
    52141 atatgaattc cgcccgaatg tgtcaacggc aaattgccac gtgtagtttc aatcgctgaa
    52201 aaatcaggca aatgaacaat tttggaaaac cgctcgcatt cagtagttat tcatgtcacg
    52261 gtttcctgta aagtggtgtt ataaaatgaa ctactaatag acccacatac attcagggaa
    52321 ttgttatgtt caaggagagg atgacgccag atgaacttgc cagactgacc ggctatagcc
    52381 gccagaccat taataaatgg gtacgcaagg aaggctggac aacgtcacca aaaccaggcg
    52441 tccagggtgg caaagctcga ctggttcacg tcaatgaaca ggttcgtgaa tatattcgca
    52501 atgctgaacg tccagaaggt cagggagaag cgccagccct ttctggtgat gcgccgcttg
    52561 aagtgttgct ggtgacactg gcgaaagaga tgacgccagt tgaacaaaaa cagtttacat
    52621 ccttgcttct gcgggaaggg attatcggat tgttacaacg cttagggatt cgcgatagca
    52681 aataatatga aaagattacg caataaaatg accaccgaag aactggctga atgcctcggt
    52741 gtggctaaac aaactgttaa ccgttggatc agagaaaaag gctggaaaac ggaaaaattt
    52801 cccggcgtga aaggcggtcg tgccagactt attctggtcg atacgcaagt ttgcgagttt
    52861 attcagaaca cgccagcctt ccataacaca ccgatgcttc tggaagccga agaacccctt
    52921 gccgaatacg caccgggtat tcgcactcct gcttatcggc aaatcatcag cgcaatagac
    52981 aacatgactc acagtgagca ggaaaaagtt gcgcaatttt tgtcacgcga aggaattcgt
    53041 aatttccttg ctcgtctcga catagatgaa tcagaataaa taaaaaacgg caggggtatc
    53101 tctcctgccg tttatctttt atgcgctaat tattgctgat tttcgcgttc agcaataaaa
    53161 gccagcgctt tgttgatacg ctcgatactg cgggtcttac cgattgcgtg aacggtaaca
    53221 tccagcgccg gagactgccc cgcaccggtt acggcgacac gcagcggcat accaacttta
    53281 cccataccca cttccagctc atccgccgtc gcctgaatag cgtgatgaac gttttcagcg
    53341 gtccagtcag taatcgcagt cagtttgtca cgaaccactt ccagcggctg acgcgctacc
    53401 ggacgcagat gttttttcgc ggcgtcggca tcgaactcag caaaatcttc gtagaaataa
    53461 cggcagctct gtgccatctc tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga
    53521 tcagccagct gcgggccgtt acgggtatcg atattttcct gctcaatgtg ccattgtaag
    53581 tgagtagcaa catactccgg cggcagcgcg ttaatgtagt gatggttcag ccacagcagc
    53641 ttgtcggtgt tgaacgcact ggcagatttg ctgacggcat tcagagtgaa gtatttgatc
    53701 atctcttcac gagtgaagat ttcctgatcg ccgtgggacc agcccagacg caccagatag
    53761 ttcagcagtg cttctggcaa ataaccgtca tcacgatact gcattacgct gaccgccccg
    53821 tgacgtttgg acagtttttt accgtcatcg ccgttgatca tagaaacgtg cgcgtaaacc
    53881 ggtaccggcg ctttcagggc tttaagaatg ttgatctggc gcggcgtgtt gttgatatgg
    53941 tcttcgccac ggataacatg ggtgatttcc atatcccagt catcaacaac cacacagaag
    54001 ttataggttg gggaaccatc ggtacggcga atgatcagat catccagctc ctggttgctg
    54061 aactcaatcg ggccacggat ctgatcgtca aaaacaacag aaccttcctg cgggttagca
    54121 aaacgtacaa cgcacggttc atcatcagca tgatgctcat ggctgtggcg gcagcgaccg
    54181 tcataacgcg gcttctcacc tttcgccatt tgctcttcgc gcagcgcttc caggcgctct
    54241 ttagaacaat agcatttata agctgtgccc tcttccagca tctgatcgat caccgcgttg
    54301 tagcgatcaa aacgtttggt ctggtagtac ggaccttcat cccactccag gctcagccag
    54361 ttcatgccat ccataatggc ttcgatagct tccggcgtgg aacgctcaag atcggtgtct
    54421 tcaatacgca gcacgaactc accgccgtgg ttacgtgcaa aaagccagga gtaaagagca
    54481 gtacgcgcgc cgccaacgtg cagatagcct gttgggcttg gcgcgaagcg agttttgatt
    54541 ttcatgaaat ggccttacgt ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg
    54601 agtgggcgat attctatcac tcccgcctga ttcctcaatg tagttcgggt tgcaacctta
    54661 cgcattattg ttttcgttga caaattgcgc aacacggtct gttttgcgat catcttgctt
    54721 aaatttacga cgaacgaaca atttctttag aaaatgcgtt gactcatttt gaactctccc
    54781 tataatgcga ctccacacag cgggggtgat tagctcagct gggagagcac ctcccttaca
    54841 aggagggggt cggcggttcg atcccgtcat cacccaccaa ctactttatg tagtctccgc
    54901 cgtgtagcaa gaaattgaga agtgggtgat tagctcagct gggagagcac ctcccttaca
    54961 aggagggggt cggcggttcg atcccgtcat cacccaccac tttctcgcca gctagatttc
    55021 ttgtaacaat gtgaagtacc gaagtgggtg attagctcag ctgggagagc acctccctta
    55081 caaggagggg gtcggcggtt cgatcccgtc atcacccacc acttcgggtc gttagctcag
    55141 ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc
    55201 aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg caatttgcga aattgcctga
    55261 tgcgcttcgc ttatcagacc aactatgtcc ggcagttccc gtgcctctca tctactgcgc
    55321 caacgccgcc agcagtttaa ccagcgcggg cgtcacctgc tgcgtttcat aaacaatata
    55381 taagtctgcc gggatgcgtt ctttgagcgg acggaatatg acaccgggcc agttcatctg
    55441 cgcgtagctg tccgctatca atgtgatacc aatacccata ctgaccatgg cgagtaccgt
    55501 ttgcggttca ttaacttcgc gaataacaac cggtgaaaat cccacctgct ggcaaactcg
    55561 ctgcaaaaaa tcccagtcag tgtacacggg cggcattgta acgaaatact cgtcacgtag
    55621 cgcttccagc gggacggtgg aaaatgatga gagatgatgc tcttcaggca tcgccaccag
    55681 aaacgccgat tcatgcaacc gtaagctggt aaaaccagtc ggtggttctg tcgccattcg
    55741 ccagatcccg gcatcaagtt cgcggcgttc cagcaaggcc atttgcatcg caggcatctt
    55801 ttcgcgaaac agaacttcaa cgttaggatt ttctctaagg aatcgccgca taaccgggcg
    55861 catccgtccc cacattgccg ttcccacaac cccgagttca atccgccctg cttctccccg
    55921 acctatttgt tcaacccgag ccaatacatt atttgcattc accagcaatc gacgcgattc
    55981 ttccatcaag attttgcccg cgtgtgtcag tacgacgctg cgcgaatggc gaataaaaag
    56041 ctgcgtgccg agttgatttt ccagctcttt aatatgaatg ctgagcggag gctgagacat
    56101 atttaaacgc gctgctgcgc gaccaaaatg caactcttcc gctacggcaa gaaaataacg
    56161 gagcaactta agatctgttc tgtatacgcg ttccataatt aagtgctcca tataataaat
    56221 acatcgtatt aaattatcat ggcataaagt atttcaccac cagatatccg acatacataa
    56281 acacaatgga taatataccc ggtaatacat ttggtacagg aataggcaag cgcacgattg
    56341 taaacaaagc accaatgcca aaaccaacgc cagaagaaaa taaaatatct ttcactaatt
    56401 aacctttatc ataaaagcag ccccaaggaa cagagctgcg aatcctttta atgtgtaact
    56461 gaacgatgcg ttatcttttc agggtcatgg ttatatttaa acccaaagaa aaatatcact
    56521 gcgaggaaaa gagcataccc tgcaaacatc agccaaatgg tatgccaatc ttttacgcca
    56581 tccaccgaga agtaatccac tgccataccg ctcagaatcg agccaaccca tgcgccgaca
    56641 ccatttacca tggtcataaa aaggccctgg gcgctggcac gaatgctgga atcaacttcc
    56701 tgttcgacaa ataccgaacc agaaatattg aagaaatcga atgcacagcc ataaacaatc
    56761 atcgacagca gcagcaaaat aaacccggtt ggtgatgggt cgccataggc aaagaagcca
    56821 aagcgcagcg tccacgccac catactcatc agcatgacgg ttttaatacc gaaacgcttc
    56881 aaaaagaacg gtatcgtcag aatgaaaccg acttcagcca tctgtgaaac tgacagtaaa
    56941 atggacggat atttcaccac gaagctatca gcaaactcag gattacgggc gaaatcatgc
    57001 aggaacgggt tgccaaaaac gttggtaatt tgcagcaccg cgcccagcat catggcgaag
    57061 aggaaaaaga tagccatgcg tggattttta aacagcacga aggcatccag acccagcttg
    57121 ctggcaagcg atgtagtcgc ttttttctcc gcaaccggaa tcttcggcaa agtcagcgca
    57181 taagccgaca gcagcaatga cgcaccggac gcgatataca gctgcagact actcaattcc
    57241 agatgcagca ggcttactgc ccacatggcg acaatgaacc ccaccgtacc aaaaacgcga
    57301 atgggcggga aagcggtcac cgggtcaagc cctgcctggg caagacagga ataagagacg
    57361 ctgttcgata acgcaatagt cggcataaac gccatcgcat tgactaacat cacccaaaac
    57421 atcatatccg gatcagttac ggatgccgca taaaaaagta cgcccgcaca caccaggtga
    57481 cacagcatgt atgcacgttc tgcacgcagc catttgtctg cgatgatccc cattataccc
    57541 ggcataataa tcgcggcgat ccctttggaa ctgtaaacca tgccaacatt agcgccggtg
    57601 aaatgaaggg tattaatcat gtaagaaccg agggtaacca gccagctccc ccagataaaa
    57661 tattgcaaaa aggacattac ctttaagcgc aaagcgatgc tcataatcgt ttccttgtcg
    57721 catcatatgg cctcactcag taaggccgtt tattgttgtt ttactcaggc aattttgcgc
    57781 agaaagccgc aaataagatt aataaagttt tgctttgaga gttccgctgc tgccagcgtt
    57841 tgggcatgag aaagcttcac gtcactcaga ccttccgcca tgttggtaat cgcagagacc
    57901 gcaacgactt taagttcgca atggcgagct gaaataacct caggcaccac agacatacca
    57961 acaacatccc caccaataat ttgcatcata cgaatttccg ccgcagtctc gaaattcggc
    58021 cccggatacg agacgaacac gccctccgtc agagggaacc cctcttcttt cgccactttt
    58081 tgtaacagtg cgcggtattc cgcatcgtag gcattcgcca gcgagaagaa gcgctcacca
    58141 aaacgttcat cattaagacc caccatcggc gttcccggca tggtgttgat gtgatctttc
    58201 aatgcgacca gactgccggc ccccacttca ggacgcagtg agccagccgc attggtgcag
    58261 aacagcaact cgcagcccag caacttaaag gtacggattg catccgtcat gatggtcatc
    58321 ccacgacctt cgtagaaatg tccgcgacct ttcatgcacg ccactggcac cccctggaga
    58381 taccccagca ccagctcacc cgcatgaccg tgtacggtac ttaccgggaa cccaggcagc
    58441 ttttcgtagg aaattgcgac cgcgttctca atctgatcgg ccagcgcgcc cagcccggaa
    58501 cctaaaataa aggccactcg tggcgtgaaa tcaggtttat aagtcttgat aatatctacg
    58561 caatacagtg ggttatgaga aaactgagcc tgagacatat atatcctttt tttgtagggt
    58621 ggaatctaac gcctttttat aacgagattg ccctacctca taccaataca cttttttcat
    58681 gtatccgata gttattcgat atcgatatat ttatattttt tcactattaa taggctgaaa
    58741 aaactgtatt ggtactctcc tttcccttgc attaacaata tgccgctttt aatcaacctt
    58801 ttattaagca atcaactaat aacgatcggc atagggaatt atgctatgaa aaaacatctt
    58861 tttactctga cactctcttc tgtattggcg ataccagcag tatcccatgc agaatttaaa
    58921 ggcggttttg ctgatatagg tattcattat cttgactgga ctagcagaac cactgagaaa
    58981 tcgtcgacca aatcacacaa agatgatttt ggatatctgg aactggaagg cggcgctaac
    59041 tttagttggg gagaaatgta cgggttcttc gactgggaaa acttttataa tgatcgtcat
    59101 gataaaccgg gtagcgagca gcgttatacc tttaaaaata ccaaccgtat ttacctgggt
    59161 gacaccggat ttaatctcta cttgcatgcg tatggcacct acggttctgc gaatcgcgtg
    59221 aatttccatg acgatatgtt cctttacggt atcggctaca atttcaccgg tagcggttgg
    59281 tggttcaaac cgttctttgc aaaacgctat acagaccaaa cctattatac cggcgataac
    59341 ggttatgtcg ccggttgggt tgcaggttac agctttatgc tgggcagtga gaaattcacc
    59401 ctcaccaact ggaacgagta cgagtttgat cgtgatgcca cctacgcggc aggtaatggc
    59461 ggcaaagaag gtctgaacgg tgccgtcgca ctttggtgga atgcaacttc acacatcacc
    59521 acaggaattc agtatcgtta tgcggatgac aaactgggcg aagattttta ccaggatgca
    59581 ataatctact ccatcaaatt taatttctga ttgattactg ccggggctcc cggcagtatg
    59641 aatagctcta tatctgatac agcggatccc cttcccgccc agccattagc gcacggccct
    59701 ggcctcgtac cacttcccat aacgcttgtg cggcggtgga aagtgaacgg ttcttcctgc
    59761 gcaccagcat caactggcgt tccaccaccg gcgtaatgcg ctttaccacc agcgggctac
    59821 cttcaggtaa cggcagcgcc agtgcgggaa gaatactaat gccgatgcct gcggctacca
    59881 tcgggaacag cgtcgccgga tgaccaatct cctgcacaat attcgcctga ataccgttgc
    59941 gcgccagtgc tgcatcaatc agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt
    60001 tcgctccctg taatgcctgc cagggtacat aatcttccgc ggccaaagcg ctatcgcgat
    60061 ggcaaagcaa gaagaaaggc tcggaaagaa tcgcttcaca ttgcagatcg ctcactgggc
    60121 ctggatcgat gacgatgcca aaatcgacat ctccctggcg aatactttcc atcacccact
    60181 gctgcggacg atcgtgcagg ataaactgaa tatctggata gcggcgatgg ctttcagcaa
    60241 tgcattgcgg aataagatgc gcggaaatgg tctggctggc agcgacccga acttttccgc
    60301 tcagttgttg ccccatacgc ccggtatcgc gcaacgtgct gttaagttca tccagcagtc
    60361 gctcaagacg caaagccaac tgctgccctg catctgtaag caccacttca cgcgtggttc
    60421 tgtccagcag gcgaacacca gtatgatttt ccagctcctt cacactgtga ctcactgccg
    60481 actggctcag gccgatacgc tctcctgcac gactaaaact tttctcctgc gctactgtga
    60541 cgaaaacctt taattgtttt aaagaataat tcatcttttt tcttcatgaa tggatgcaat
    60601 aaatcaattt tatttctcaa actggaagaa gcacaataga accatcgatc atctggagtc
    60661 tttatgaaac tttttcgtat cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg
    60721 ctggcctctt tctttccggc cagaggcgat ttcgtcccct tctttgaaaa tctgaccact
    60781 gcagctattg ccctgctgtt ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt
    60841 gctggcggtg ggcactggcg actgcatttg tgggtaatgt gcagcacctt cgtattgttt
    60901 ccgattctgg gcgtgctgtt tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac
    60961 tccggttttc tctacttgtg cattctcccc gctaccgtgc agtctgcaat cgccttcacg
    61021 tcaatggcgg gcggtaacgt tgcggcggcg gtttgttctg cgtcagcatc cagcctgctt
    61081 gggattttcc tttcaccatt gctggttggt ctggtgatga atgttcacgg tgcgggaggc
    61141 agccttgagc aggtcggtaa aattatgctg caattgctac tgccgtttgt gttggggcat
    61201 ctttcccggc cgtggattgg tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact
    61261 gaccagacgt ccattctgtt ggtggtttat acggcattca gcgaagccgt cgttaacggt
    61321 atctggcaca aagttggctg gggatcattg ctgtttatcg tggtggtcag ttgcgttctt
    61381 ctggctatcg tgattgtagt taacgtcttt atggcacgcc gactgggctt caataaggca
    61441 gatgaaatta ctatcgtctt ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg
    61501 gcaaacattc tgttccccac atcggtgatc ggtatgatgg tgctgcccct gatgattttc
    61561 catcagatcc aattgatggt ctgtgcggtg ctggcgcgtc gatacaaacg ccagaccgaa
    61621 cagttacagg cgcagcagga aagcagcgcc gataaagctt aaagcggacg cttcaggggc
    61681 tggaccagtt gcgtcagccc ctcggtttta atcagcagcg tgatttgcat tagctcaccg
    61741 agcttccctg ccgggaactc atctttgcgg gcaaaccaca gcagatactc ctccggcagg
    61801 tcgattaagc gacgcccttt gtacttgcca aacggcatta tcgtattggc tatttcaatc
    61861 agctgctctt tttccatctc agctacccag caaacgcagc atttccgttt cgtcgatgac
    61921 ttcaataccc agttcctgcg ccttcgccag tttagatcct gcagcttcac ccgctatcac
    61981 cagatcggtt ttcttcgaca cgctgcccgc gactttcgcg cccagttcga ccagtcgagc
    62041 tttagcgtca tcacgcgaca tctggcttaa gctgcccgta agcaccacgg ttttaccagc
    62101 aaacgggctg tcaatctctt ccgcgttaat caccaccggc gcaggccagt gaacgccttc
    62161 cgccaacagc tcgctgatga cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg
    62221 ggaggcaacg acaatgccaa catcaggcac cttttgcagc tcttcaatcg aagcggcttc
    62281 cagcgcttcc agcgtgccga aatatgccgc cagacctgct gcggtggcct cgccgacttc
    62341 acggatgcca agtgcataga ggaagcgagc aaaggtggtt tctttcgctt tttccagcgc
    62401 gttaaccacg ttttgtgccg atttcggccc catacgctcc agtccggtca gtttgactgc
    62461 ggtgagtttg aacagatccg ccggagtgtg gacatattct ttttcaacca gttgatcgat
    62521 gattttgtcg cccattccgt caacatccat cgcacggcgg gaaacaaagt gtttcagaga
    62581 ctctttacgc tgcgcaccgc aaatcaggcc accggtacag cgggcaaccg cttcaccttc
    62641 cacacgctcg acgtcagaac cacataccgg acaatgcgtc gggaatacaa cctcacgggt
    62701 atcttccggg cgttcagaaa gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg
    62761 gcgaataacc actttatcgc caatgcgtaa accaagacgt tcgatttcgt cagcattatg
    62821 cagtgtagcg ttactcacca acacgccagc aacatggaca ggttccagac gcgcaacagg
    62881 cgtaatcgcg ccagtacgcc caacctgaaa ctcgacgtca cgcacaaagg tcatctgttc
    62941 ctgcgccggg aatttaaacg ctaccgccca gcgcggggca cgcgcgacaa agccaagctg
    63001 ctcctgctgt gccagtgagt tgaccttaat caccacgccg tcgatatcaa agcccagcgt
    63061 cggacggtct ttttctactt cgtgataaaa cgccagcact tcttccgccg attcacaaag
    63121 tgttacccga tcactgacag gtaatcccca cgctttaaat tgcattaagc gtcccaaatg
    63181 ggtatccggc aattcaccgc cttccagaac accaacgcca tagcagaaga aagtgagcgg
    63241 ccgcttcgct gtaatacgcg gatcaagctg acgcagtgaa ccagctgccg cattacgtgg
    63301 gttagcaaac actttcccgc ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc
    63361 ctgcggcagg aacacttcac cacgcacttc cagacgcgcc gggatattct ctccgtgtag
    63421 cttcagcgga atggcgcgaa tagtacgcac attagacgtg atatcttccc cggtggtgcc
    63481 atcgccacgg gtggcggcac tgactaaaac accattttca tacagaatac tgacggcaag
    63541 accatccagc ttcagctcac agcaccaggt gactttctcg ttgcttttca gacggtcctg
    63601 cacacgtttg ttgaaagcaa gaaagctttc ttcatcaaaa acgttatcca gcgacagcat
    63661 tggcacttca tggcggatct ggctgaaagc cgccagcggc gcagcaccta cacgttgggt
    63721 aggcgaatca ggcgtaatca gttctggatg tttggtttcc agctcgcgca attcgcgcat
    63781 cagcctgtcg tattcagcgt cgggaatttc cggcgcatcc atcacatgat aaagatattc
    63841 atgatggcga agcgtcgttc gcagttctgt cagttgttgt tcgattgatt ccatatcgca
    63901 ccatcaatgc taaaaacccc cgacaagcgg gggttcgaag aggagttaat ttgccttaag
    63961 tgtatcaggc gttggcgtct ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct
    64021 gcggagtcat catacggcgc tgatcgtcaa gaacaacacc gcccacttca tcggcaatat
    64081 gctgcgcaga ttgcagcatc agcttgaagt tctgcagctc gtcaccgtaa gacggtacct
    64141 gcataaagat agtgacaccc ggagtagtga aatccttcat ttcaggatca aaggttcccg
    64201 gtttcaccat attcgccagg ctgaataacg ccgggccgct gccatccggg ctaagatgac
    64261 gatggtaaat attcatatcg ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca
    64321 gttcaccgtt tagctcgcta ccgtgatgcg ccgcgacgtt catgataatc accgcttctt
    64381 tgcgcttcgg tttatccata actggagccg gttccgctac aggctcaggc tgtggtgccg
    64441 ctacgggttc tgcaggctgg aaagcctgtt gtgccggttg cggtgctgaa tgcactggct
    64501 gcggcgctgg cgcgacctgt ggcgaaactg gctgctgcaa cggctgttca ggctgcggct
    64561 gataggcagg ctgctgcacg ggttgctgca ccagctgcgc tggacgcgga gcatgttgcg
    64621 gcggtacctg cgcttcaggc ggctgctgga ccggttgacg cggctgcgca gacgcataag
    64681 gcggttggta ctggtgttgc ggcgacggac gagcagcctc atgctcctgg gcgttagccg
    64741 gggcatgatt cacgcggtga actcgaacct caccaacgcc ctcatcatct tcgacatcct
    64801 cgtcataaga atcgtcgtca cgttttgact tcattcgttt taatggccga tcgcggaaca
    64861 tagaagatcg ttctttacgg ctggtccaga aaccatgtac cagtaaagcg attatggcga
    64921 tcgcgccaac aatgattaat atcagacgca aatcctgcat cattatattc tctgttgttc
    64981 taacaccttg ccaccacggc aaacatttac tcactaagag tatttgtcga ttacctcaag
    65041 tgcaagtgca ctattaactt tcacagcaca aagatagatg aaatcgtgct ttttgctgtt
    65101 ttttcgaaca tatcctaact gtccattgcg caattacccg gtaaaatacg cagaattttc
    65161 ctgggattgg tcaaaaggag ctcatcctga ttatggtttc atcattcaca tctgccccac
    65221 gcagcggttt ttactatttt gcgcaaggct ggaagctcgt ctcgcaacct gggattcggc
    65281 gtttcgttat tttaccgctg ctggtcaata ttttgttgat ggggggcgca ttctggtggc
    65341 tctttacgca actcgatgtc tggatcccga ctctcatgag ttatgttccg gactggttac
    65401 aatggctgag ctatctgttg tggcccctgg ctgtcatctc tgtgctgtta gtatttggtt
    65461 atttcttttc aacgattgct aactggattg ccgctccgtt caacggttta ttggctgaac
    65521 aactggaagc aagactgact ggcgctacac cgccagatac cggaattttc ggtatcatga
    65581 aagatgtgcc gcgaatcatg aaacgcgaat ggcaaaaatt tgcctggtat ctgccgcgcg
    65641 caattgtatt gctaattctt tacttcatcc ctggtattgg gcaaaccgtc gcgccagtac
    65701 tgtggttcct gtttagcgcc tggatgttag ccatccagta ttgcgattac cccttcgata
    65761 accacaaagt gccgtttaaa gagatgcgca ccgccctgcg cacacgcaaa atcaccaata
    65821 tgcagtttgg tgctttaacc agcctgttta cgatgatccc gttgcttaat ctgttcatca
    65881 tgcccgttgc cgtttgtggc gcgacggcga tgtgggtcga ttgctatcgc gataaacacg
    65941 cgatgtggcg gtaacaatct accggttatt ttgtaaaccg tttgtgtgaa acaggggtgg
    66001 cttatgccgc cccttattcc atcttgcatg tcattatttc ccttctgcat atagatatgc
    66061 taaatcctta cttccgcata ttctctgagc gggtatgcta cctgttgtat cccaatttca
    66121 tacagttaag gacaggccat gagtaagatt tttgaagata actcgctgac tatcggtcac
    66181 acgccgctgg ttcgcctgaa tcgcatcggt aacggacgca ttctggcgaa ggtggaatct
    66241 cgtaacccca gcttcagcgt taagtgccgt atcggtgcca acatgatttg ggatgccgaa
    66301 aagcgcggcg tgctgaaacc aggcgttgaa ctggttgaac caaccagcgg taataccggg
    66361 attgcactgg cctatgtcgc tgccgctcgc ggttacaaac tcaccctgac tatgccagaa
    66421 accatgagta ttgaacgccg caagctgctg aaagcgttag gtgcaaacct ggtgctgacc
    66481 gaaggtgcta aaggcatgaa aggcgcaatc caaaaagcag aagaaattgt cgccagcaat
    66541 ccagagaaat acctgctgct gcaacaattc agcaatccgg caaaccctga aattcacgaa
    66601 aaaaccaccg gtccggagat ctgggaagat accgacggtc aggttgatgt atttattgct
    66661 ggcgttggga ctggcggtac gctgactggc gtcagccgct acattaaagg caccaaaggc
    66721 aagaccgatc ttatctctgt cgccgttgag ccaaccgatt ctccagttat cgcccaggcg
    66781 ctggcaggtg aagagattaa acctggtccg cataaaattc agggtattgg cgctggtttt
    66841 atcccggcta accttgatct caagctggtc gacaaagtca ttggcatcac caatgaagaa
    66901 gccatttcta ccgcacgtcg tctgatggaa gaagaaggca ttcttgctgg tatctcttct
    66961 ggagcagctg ttgctgcggc gttgaaacta caagaagatg aaagctttac caataagaat
    67021 attgtggtta ttctgccgtc atcgggtgag cgttatttaa gcactgcatt gtttgccgat
    67081 cttttcactg agaaagaact gcaacagtaa tgccagcttg taaaaaatgc gtaaaaaagc
    67141 acctttttag gtgctttttt gtggcctgct tcaaactttc gcccctcctg gcattgattc
    67201 agcctgtcgg aactggtatt taaccagact aattattttg atgcgcgaaa ttaatcgtta
    67261 caggaaaagc caaagctgaa tcgattttat gatttggttc aattcttcct ttagcggcat
    67321 aatgtttaat gacgtacgaa acgtcagcgg tcaacacccg ccagcaatgg actgtattac
    67381 gctcttcgtg cgtcgcgtct gttaaaaact ggcgctaaca atacaggcta aagtcgaacc
    67441 gccaggctag actttagttc cacaacacta aacctataag ttggggaaat acaatgttcc
    67501 agcaagaagt taccattacc gctccgaacg gtctgcacac ccgccctgct gccctgtttg
    67561 taaaagaagc taagggcttc acttctgaaa ttactgtgac ttccaacggc aaaagcgcca
    67621 gcgcgaaaag cctgtttaaa ctgcagactc tgggcctgac tcaaggtacc gttgtgacta
    67681 tctccgcaga aggcgaagac gagcagaaag cggttgaaca tctggttaaa ctgatggcgg
    67741 aactcgagta atttcccggg ttcttttaaa aatcagtcac aagtaaggta gggttatgat
    67801 ttcaggcatt ttagcatccc cgggtatcgc tttcggtaaa gctctgcttc tgaaagaaga
    67861 cgaaattgtc attgaccgga aaaaaatttc tgccgaccag gttgatcagg aagttgaacg
    67921 ttttctgagc ggtcgtgcca aggcatcagc ccagctggaa acgatcaaaa cgaaagctgg
    67981 tgaaacgttc ggtgaagaaa aagaagccat ctttgaaggg catattatgc tgctcgaaga
    68041 tgaggagctg gagcaggaaa tcatagccct gattaaagat aagcacatga cagctgacgc
    68101 agctgctcat gaagttatcg aaggtcaggc ttctgccctg gaagagctgg atgatgaata
    68161 cctgaaagaa cgtgcggctg acgtacgtga tatcggtaag cgcctgctgc gcaacatcct
    68221 gggcctgaag attatcgacc tgagcgccat tcaggatgaa gtcattctgg ttgccactga
    68281 tctgacgccg tccgaaaccg cacagctgaa cctgaagaag gtgctgggtt tcatcaccga
    68341 cgcgggtggc cgtacttccc acacctctat catggcgcgt tctctggaac tgcctgctat
    68401 cgtgggtacc ggtagcgtca cctctcaggt gaaaaatgac gactatctga ttctggatgc
    68461 cgtaaataat caggtttacg tcaatccaac caacgaagtt attgataaaa tgcgcgctgt
    68521 tcaggagcaa gtggcttctg aaaaagcaga gcttgctaaa ctgaaagatc tgccagctat
    68581 tacgctggac ggtcaccagg tagaagtgtg cgctaacatt ggtacggttc gtgacgttga
    68641 aggtgcagag cgtaacggcg ctgaaggcgt tggtctgtat cgtactgagt tcctgttcat
    68701 ggaccgcgac gcgctgccca ctgaagaaga acagtttgct gcttacaaag cagtggctga
    68761 agcgtgtggc tcgcaggcgg ttatcgttcg taccatggac atcggcggcg acaaagagct
    68821 gccatacatg aacttcccga aagaagagaa cccgttcctc ggctggcgcg ctatccgtat
    68881 cgcgatggac cgtaaagaga tcctgcgcga tcagctccgc gctatcctgc gtgcctcggc
    68941 tttcggtaaa ttgcgcatta tgttcccgat gatcatctct gttgaagaag tacgtgcact
    69001 gcgcaaagag atcgaaatct acaaacagga actgcgcgac gaaggtaaag cgtttgacga
    69061 gtcaattgaa atcggcgtaa tggtggaaac accggctgcc gcaacaattg cacgtcattt
    69121 agccaaagaa gttgatttct ttagtatcgg caccaatgat ttaacgcagt acactctggc
    69181 agttgaccgt ggtaatgata tgatttcaca cctttaccag ccaatgtcac cgtccgtgct
    69241 gaacttgatc aagcaagtta ttgatgcttc tcatgctgaa ggcaaatgga ctggcatgtg
    69301 tggtgagctt gcaggcgatg aacgtgctac acttctgttg ctggggatgg gtctggacga
    69361 attctctatg agcgccattt ctatcccgcg cattaagaag attatccgta acacgaactt
    69421 cgaagatgcg aaggtgttag cagagcaggc tcttgctcaa ccgacaacgg acgagttaat
    69481 gacgctggtt aacaagttca ttgaagaaaa aacaatctgc taatccacga gatgcggccc
    69541 aatttactgc ttaggagaag atcatgggtt tgttcgataa actgaaatct ctggtttccg
    69601 acgacaagaa ggataccgga actattgaga tcattgctcc gctctctggc gagatcgtca
    69661 atatcgaaga cgtgccggat gtcgtttttg cggaaaaaat cgttggtgat ggcattgcta
    69721 tcaaaccaac tggaaacaaa atggtcgcgc ctgtagacgg caccattggt aaaatctttg
    69781 aaaccaacca cgcgttctct atcgaatctg atagcggcgt tgaactgttc gtccacttcg
    69841 gtatcgacac cgttgaactg aaaggcgaag gcttcaagcg tattgctgaa gaaggtcagc
    69901 gcgtgaaagt tggcgatact gtcattgaat ttgatctgcc gctgctggaa gagaaagcca
    69961 agtctaccct gactccggtt gttatctcca acatggacga aatcaaagaa ctgatcaaac
    70021 tgtccggtag cgtgactgtg ggtgaaaccc cggttatccg catcaagaag taattcttgc
    70081 cgcagtgaaa aatggcgccc atcggcgcca ttttttatgc ttccgccagc ggcggcaaaa
    70141 tcaattcatc gctctcatgc tgctgggtgt agcgcatcac ttccagtacg cgcaaccccg
    70201 cacggtgcac cgcatcgttt aacgccttcc ctttcagcaa tccactgata agctgagcac
    70261 aaaacaggtc gccagtccct ttcaggtcgg tttttacccg tgaatgggaa atgacattca
    70321 cgctgtcggc actgaccacc accacctgca tctcctgatt ttcttcatta ccggaggcgc
    70381 tggtaatcac cacccatttt aatgtgtctg aaagcagact ttttgcggca gcaatggcac
    70441 tgtcgagatc gcggcaattt ttaccggtca ggatttccag ctcaaagata ttgggggtaa
    70501 ttccctgcgc cagcggcagt aaatattgtc gatacgcctc gggaaggtca ggtttgacat
    70561 aaattccgct atcaatatcg ccaatcaccg gatcgaccat gatcaatagg tcaggatggt
    70621 ctttgcgtag cgcagtcagc cactcggcaa ggattttgat ttgcgatgcc gttcccatat
    70681 agcccgtggt tacagctcga agttggcgca gcgcatcacg ctcctgaagc gcacgcaaat
    70741 agccgctaaa ccattcatcc ggaatcgcac caccgtagaa agtgtcataa tgcggtgtat
    70801 tgctcagcaa taccgtcggc acggcaaaga cattcaggcc gttctgtttg atagcaggca
    70861 ccgcaatgct gttgcctacg ctgccgtaca ctacctgcga ctgcacggcg acgatatccg
    70921 cctgcaatgc cctgctctta tcgttaaaca acaacaaact actcatttaa ttttttctcc
    70981 ttgccgatga tcctcatcgt attccaaccg aaactttacc tgattctggc agtcaaatcg
    71041 gctatcacaa aacaaggata aggtaattca atgaagaaaa tcatttgtct ggtcattacg
    71101 ctgctaatga cactccccgt ttacgcgaag ttaactgccc atgaagaagc ccgtatcaac
    71161 gccatgctgg agggattagc acagaaaaag gatttgatat ttgtgcgtaa cggcgatgat
    71221 catacctgcg atgaagcggt ttcccacctg cgtctgaagc tcggcaatac ccgtaaccgc
    71281 attgacactg ccgagcagtt tattgataag gtagcctcgt cgtcatcgat taccgggaag
    71341 ccgtatatcg tgaagatgcc cggtaaaagc gatgagaacg cacaaccttt tttacatgcg
    71401 ctgattgcgc agacggataa aacggtgcct gcgcaataat cttaaatccc cgccccctgg
    71461 ctaaaatgct cttccccaaa caccccggta gaaaggtagc gatcgccgcg atcgcagatg
    71521 atcgccacaa ccaccgcgcc ggggttagct tttgccacgc gcagtgctcc ggcaaccgcg
    71581 ccgccggagc tgacgccaca gaatattcct tcccgcactg ccagttcgcg catggtgttt
    71641 tccgcatcgc gctgatgaat atccagcacc tcatccacca gcgaagcgtt gaaaatcccc
    71701 ggcagatatt ccgcaggcca gcggcgaatg ccgggaatgc tactgccctc ttccggctgc
    71761 aggccaacaa tggtcaccgg tttggattgt tcgcgcataa agcgtgaaac gccggtgata
    71821 gtgccggttg ttcccatgct ggagacaaaa tgggtaatgc gcccgccggt ttgctgccag
    71881 atttccggcc cggtggtggt gtaatgggcg aagggattat caggattatt gaactgatcg
    71941 agcagctttc cttcaccacg attggccatc tccagcgcca gatcgcgcgc gccttccatg
    72001 ccctgctctt tggtgacaag aatcagttcc gcaccgtatg ccagcatcgc tgcacggcgc
    72061 tcctggctca tgttgtcggg catcagcaat ttcatgcgat agcctttaag cgcggcaatc
    72121 attgccagcg caatgccggt gttaccactg gtggcttcaa tcaggacatc gcccggtttg
    72181 atttctccgc gcttttccgc ctcgacgatc atcgaaagcg ctgcacgatc tttcaccgaa
    72241 cctgccgggt tattgccttc cagtttcaac cagacttcac tgccgttatc cggcgccatt
    72301 cgctgcaact tcaccagagg cgtattgcca attgtttgtt ctaatgtact cacgatctct
    72361 gtccatacgt ggtgttgcct gatgcgacgc ttgcgcgtct tatcaggcct acaggttaca
    72421 aaccttgcca ttaaaaagcc cggatcgcgg gaagcgcctc cgggcgttta acattcactc
    72481 aacctatcag gcgctttgtg cgagagcaag ttcctcatcg cgggtttcaa tacgctcgtc
    72541 gccgttatac agccgcgcat gttgcagacc aacgaataaa cgctcgccac gctgcggggc
    72601 atcgtcgcca tgcatcacaa ccgtcagcgg ttcgttgtac caccccagcg gctgcacgac
    72661 taattgggtg tagtggcctt tcgggctggc ttccagtacc tgtaccggca gcggcgaatc
    72721 gaggctggta cggcggctga tatccacttc ccacggacgc aggaagagat ccaccggccc
    72781 ctgatacgca ggtgtatagc ccagcggcca gcgatgtgcg ccaacatgga actgcccgcc
    72841 gcgaatggtt ccctgcaggc ggttcacttc gcccataaat tcgagtacaa aacgggtcgc
    72901 tggttcgcgc catacctggt ccggtgcatc agcctgctcg atattgccct ggctcatcac
    72961 cactacacga tcggctactt cggtcgcttc ttcctggtca tgggtcacaa acacgctggt
    73021 gaatttcagc tcttcatgta actgacgcag ccagcgacgc aactctttac gcacctgcgc
    73081 atccagcgcg ccaaacggtt catcaagcag cagaatttgc ggttctacag ccagcgcgcg
    73141 cgccagtgcc acacgctgtt tctgaccgcc ggaaagctgt gctggataac gatccgccag
    73201 atgcgcaagc tgcaccatct ccagcaattt tgtcactttc gctttgatgg ctgcggcatt
    73261 cgggcgctcg cgacgcggca gcaccgtcag gccgaaagcg atattgtcga acaccgtcat
    73321 atggcggaac agcgcatagt gctggaacac gaaaccgact ttacgatcgc gcgcatgcag
    73381 gcggctgacg tcggtgccgt ggaagcgaat atgcccgctg gtttgatgct ccagcccggc
    73441 gataatgcgc agcagcgtgg ttttcccgga accggacggc cccagcaacg cgaccatctg
    73501 accagaagga atatccagtg agatatcgtt cagcacctgg gtgcgaccaa aagacttctt
    73561 aatattggca atctcaatgc tcatgatgtt cctcctgctg cgcgcgtttt tcctgattct
    73621 ccagacgcca ctgcaacata ctctttaaaa acagggtgat aatcgccatc agcgtcaaca
    73681 acgcggcagc ggtaaaggag ccgacggtgt tgtagtcttg ctccagcaat tcaatctgta
    73741 acggcagcga aagtgtttcg ccgcgaatcg agccggaaac caccgacacc gcaccaaact
    73801 cgccaatcgc gcgggcgttg gtcagcacca cgccataaag cagcgcccag cggatgttcg
    73861 gcagcgtgac gcggcgaaac atctgccagc cagacgcgcc aagcaaaatc gccgcttcgt
    73921 cttcctggct gccctggctt aacatcaccg gcaccagctc acgcaccaca aacggacacg
    73981 tcacgaagat ggtgaccagc accattcccg gccaggagaa cataatttgc agattatgtt
    74041 catccagcca gccgcccagc gggccgttag agccgtagaa cagcagatac accagaccag
    74101 caaccaccgg cgacacggca aacggaatgt ccaacagcgt cagcaatagc tggcgtccgg
    74161 ggaagttaaa gcgcgtcacc agccaggcca gcagaatacc gaacaccagg tttaccggta
    74221 cggcaatcag cgcgatcatc accgtcagcc agatggcgtg cagcatgtcc ggatcggcca
    74281 gattctgtaa aaccggcatc agccctttgc tgaacgcctg tacgaagata taaatcatcg
    74341 gcaccagcag gatgaacgcc gataccagca tcccgatgcc aatcagaaac catttgcccc
    74401 agttaatcgg gcgcgcgtca taacgcttca actgtgtcac ttccgccatt aatgacctac
    74461 cacacgccga ccaaagcgac tttgcagagt gttaattgag aacagcagca gcagcgatgc
    74521 cgcgaggatc accgaggcaa tcgcgctcgc cgccgggtaa tcaaactcct gtaagcgaac
    74581 aaagatcatc agcgacgtca cttctgtttt ccaggcgata ttcccggcga taaaaatcac
    74641 cgcgccaaac tcaccgaggc tgcgggtaaa cgacagcgcc acacccgcca ccagcgccgg
    74701 agatagttcc ggcagcacca ctttgcgaaa actctgccag cgtgttgcgc cgagcgtttc
    74761 tgccgcctct tcatattccg ggcctaactc ttccagcacc ggctgcacgg tgcgcaccac
    74821 aaacggaatg ctggtaaagg ccatcgccac cgcaatcccc agccaggtat aggtgacttt
    74881 gatatcaaac ttcgccagcc attcaccgta aaaaccgttc acggaaaaga gcgaggccag
    74941 cgttaaaccg gcgactgccg ttggcagcgc aaagggtaaa tccatcagcg catcaagcag
    75001 cgtgcgaccc ggaaagcgat agcgggttaa gatccacgcc atcagcagac cgaaaacgcc
    75061 gttaaaaatc gatgccacaa acgccgacag cagcgtcact ttataggctg cgaccacctg
    75121 cgggttggtg atcacctccc agtactgcgc ccagctcatc tgggccagtt gcatcaccag
    75181 cgcggagagc ggcagcagca aaatcaggca cacaaacagc agactggtgc cgaggcttaa
    75241 ggtaaagccc ggcagcacgc gtcttgaaga aactgcaaac attacttacg ccccgccgct
    75301 aacagcttgt ctaactcgcc gccgctggtg aagtgggttt tcatcacttc cggccaggag
    75361 ccaaatttat cttccacgcg gaacagctcg gtctgcggga atttatcttt cagtttgccc
    75421 atgacttccg ggttatttac gcggtagtaa tagtcggtga tgatggtttg cgcctgcggg
    75481 ctgtagagcc agttcaggta ggcttttgcc gctttttccg tgccgttggc ctgtacgttt
    75541 ttatccaccc acgccaccgg gaattccgcc agaatgttgg ttttcggaat caccacttca
    75601 aagccctgcg cttcatactg tttacggatg ttgttcactt ccgactcgaa gctgatcagc
    75661 acatcgccca ggccgcgctc ggcaaaagtg gtggtcgcgc cacgaccgcc agtatcgaac
    75721 acttcaacgt ttttcaggaa ctgggtcata aactgttcgg ttttggcttt atcaccaccg
    75781 tcggctttgt ccgctgcgcc ccatgccgca aggtaggtat aacgcgcgtt acccgacgtt
    75841 ttcgggttcg ggaaaatcag cttcacgtca gagcgtacca ggtcgttcca gtcgtggata
    75901 ttctttgggt tgcccttacg caccaggaag cccatggtgg agtagaacgg cgagctatta
    75961 ttcggcaggc gcgactgcca gtccgctggg atcagcttgc ctttatcgtg caggatttgt
    76021 acgtcggtca cctggttata agtgacaacg tcggctttta aaccctgcaa aattgccagc
    76081 gcctgttttg atgatccggc atgagattgt ttaatcgtca gtttgtcgcc accgttatct
    76141 tttgcccatt gttgctcaaa cggcgggttc agggcggcaa acagctcgcg ggagacgtca
    76201 taagaactgt tcagcagttc cgttgcctgt acatggcccg ccagtagcag agaagcaacc
    76261 agcacgagtg agttcttttt cagtaagtta acggccattg cgcaccctta taaatttaat
    76321 gactttctaa tagccatcat atttataacg gacgtgaaag gagtaacggt tttatatacc
    76381 gtttggtgat ttggaagttg aaaagggaat aagacgaaat cctccccaca aatgcaggga
    76441 ggaaacagag gtgaatcaga tgccgacgct aaccgtctcc ggcagtgtgc tgccgccatc
    76501 aatcacattc tgtgtaccgg ttaaatagct ggattcatcc gatgcgagga aggccgccag
    76561 ttcgccgact tccagcggat cggcgaggcg acgcatcggg attgctttcg ccatttcagt
    76621 cagcaccgat tctggatctt ccgggttcga ctggcgggca atgctttccg ccattggcgt
    76681 gcgaacgtat ccagggcaga tggcgttaac gcgaatacca gactgcgcgt actccaccgc
    76741 cagcgattta gtcaggccaa caatcgccgc tttcgttaag gcgtacgccg tttcgcccgg
    76801 gtctgccacc atatcgccag tgactgaaga catcatcaca atccggccat ctttgcgggc
    76861 aatcatctcc ggcagcaccg ccttcgtgac gttccatacg ccttttatat tgatatcaat
    76921 atggaaatcg cgatcttcat cgctcatatc aaggaaactg cccagacgac aaacgcctgc
    76981 gttattcacc agaatatcaa tgcgcccttc tttttcctta gcgcgcttaa tagctgcggc
    77041 gaccgacgcc gggtcacgca catcggcgac aaccgccgta cagcgatgac cacggccaca
    77101 cagttcgtcc gccagctttt cgatctcagg ggagatatcc agcaagatta ggtttgcgcc
    77161 atgacgcgca aaagttctgg caattccttc gccaattccc tgcaatgcgc ccgtaatcag
    77221 tgctgtcttg cccgtgagtt tacccatttt aatgctctcc ttgttaatac agagcgttac
    77281 actccattcc ctctgaaaat acaggataaa caaaatattg ctgcggatca gctcactaat
    77341 tcaggaaaat ctcaatccag tcgctgcttt atgcatcatt tcaggcgttg cgttagctca
    77401 ctgctgcgct caatcatttt caatgactcc acatcattga gttgcacaag gccaacaaac
    77461 agtaaatcgg taacagaatt ttgcgccgtg cgggtggaca tcgacgagct gcgccattcg
    77521 gtttcgccag aaacggtatc gagggtgtaa tgcgccagcc gccgcagcgg ggaatcggtt
    77581 aatgaggtaa tggcaatcac cgttgccccc tgctttcgcg ctgcctcggc gcacaagaca
    77641 atctcttttt tactgccact gtaagagata gcgatttgta catcgccttt ttttagcgcc
    77701 tgagaaacgg tggcttgtac gtgggtgtcg gcctcacagg ccacgcgata accaattttc
    77761 atcagtttga acgacagatc gcgccccacc agctccgacc cgcccaggcc cgttatctgg
    77821 ataaacggtg ctttactgat gacctcaatg attttttgca gccgcgcgta gtcaaacaac
    77881 gcgcaggttt gctccagcgc ctgctctttt tcgcgattca gtttacgggc aatcacttcc
    77941 agcgaatcat cactggtaat cgaactgtgc aggtgcagcg ccgtggcatt cgttttctcc
    78001 cggctggcac tgtattcgcc aattaacgcc atacgcaatt cagtaaatcc ttgcgcgccg
    78061 agcttctggg caaacttcac aatactcgac tggctgatgc cgagctgttt cgccatcttg
    78121 cgcgaagaga cggatttcag ctcactcacg ttggcccgca gaaagtcagc aattttttgc
    78181 tcgttcccgg taaattcact ttccgcattg cgtattttcg tcaggtacaa catcttccac
    78241 tctcctgggc tggcagcaaa aattccgctt aagttcttat gacgctcttc acactctgcg
    78301 agtgtagact caatgattcc tttaagtctg tcaattcggg atatatgatt ccatacatct
    78361 gcaattattt gatcgtaaat agtaaggtca ccaccgatgc aacttgaaaa gatgattacc
    78421 gaaggctcga acgccgcttc ggctgaaatt gaccgcgtat caacgctgga aatgtgccgg
    78481 attatcaacg atgaagataa aaccgtacca ctcgccgttg agcgcgtact gccggatatc
    78541 gccgcagcga tcgatgttat ccacgcccag gtcagcggcg gcggtcgtct gatttatctc
    78601 ggtgcgggaa catcaggtcg tctggggatt ctggatgcca gcgaatgtcc gccgacctac
    78661 ggcgtgaaac cgggtctggt ggttggatta attgctggcg gcgaatatgc cattcagcac
    78721 gcggtggaag gcgcggaaga tagccaggaa ggcggtgtga atgatctgaa aaacatcgga
    78781 ttaaccgcgc aggatgtggt ggttggtatt gccgccagtg gtcgcacgcc gtatgtgatt
    78841 gccggactgg aatacgcgcg ccagctcggc tgccgcacag tgggaatttc ctgtaatcca
    78901 gggagcgcgg tttccaccac cgctgagttt gctattacgc cgatcgttgg tcccgaggtg
    78961 gttaccggtt cttcacgaat gaaagcaggc acagcgcaga aactggtgct caatatgctc
    79021 tccaccgggc tgatgattaa atccggcaaa gtgttcggca acctaatggt cgatgtggtc
    79081 gccaccaacg aaaaattgca tgtgcgccag gtcaatatcg tcaaaaacgc taccggatgt
    79141 agcgcagagc aagcggaagc agcgttgatt gcctgcgagc gcaactgtaa aacggccatt
    79201 gtgatggtgc tgaaaaatct cgatgccgca gaagctaaaa aacgcctgga tcaacacggc
    79261 ggctttattc gtcaggtttt agacaaggaa taacccatgg ccaaagagat cagcagtgaa
    79321 cttctgaaca ccattcttac ccgggtcggt ggaccgggaa atatcgccag ttgtggtaac
    79381 tgtatgacgc gcctgcgtct gggtgtacat gacagttcac tggttgatcc caatatcaaa
    79441 acgctggaag gcgtgaaggg cgtcatttta accagcgacc aggtgcaggt cgtttttggg
    79501 cctggtaaag cgcatcgtgc cgcaaaagcg atgagcgagc tgctgggaga agcaccggta
    79561 caggatgccg cagaaatcgc cgcccagaac aaacgtcagt taaaagccaa acaaacctcc
    79621 ggcgtgcaac aatttctcgc caaattcgcc accatcttca cgccgctgat ccccggtttt
    79681 attgccgctg gtctgctgct ggggatagcg acgttaattg ccacggtgat gcacgttccg
    79741 gcagatgctc agggcacgct acccgatgcg ctgaatttta tgaaggtgtt cagcaaaggc
    79801 ctgttcacct tcctggtgat tctggtgggc tataacgccg cccaggcatt tggcggcacg
    79861 ggcgtcaatg gcgcaattat cgccgcgctc tttttgctcg gttataaccc aactgcaacc
    79921 accggttact acgccggatt tcacgatttc tttggtctgc ccatcgatcc gcgcggcaat
    79981 attatcggcg tgttgattgc cgcgtgggcc tgcgcccgca tcgaaggcat ggtgcgccgc
    80041 tttatgccgg acgatctcga catgctgttg acctcgttaa tcaccctgct aatcaccgct
    80101 acgcttgcgt acctgatcat tatgccgctg ggcggctggc tgtttgaagg catgtcgtgg
    80161 ctgtttatgc acctgaacag taatccgctc ggttgtgcag tattagccgg gctgttcctg
    80221 atcgccgtgg tgtttggcgt gcatcagggc tttattcccg tttacctcgc gttaatggac
    80281 agtcagggat tcaacagctt atttcccatc ctttcaatgg caggcgcggg ccaggtgggc
    80341 gcggcgctgg cactctactg gcgggcgcaa ccgcacagtg cgctacgcag ccaggtgcgc
    80401 ggggcgatta ttcccggtct gttgggcgtt ggcgaaccgc tgatttacgg cgtcaccctg
    80461 ccccgcatga aaccgtttgt taccgcctgt ttaggtggcg cggcgggtgg tttgtttatc
    80521 gggctgatag cctggtgggg cctgccgatg ggcttaaaca gtgcctttgg gccgtctggt
    80581 ctggtggcgc tgccgctgat gacatcagca caaggtatcc ttccggcaat ggcggtttat
    80641 gcaggcggga ttctggtggc atgggtttgc gggtttattt tcaccacgct ctttggctgc
    80701 cgtaacgtca atctggactg ataatatgaa acggacaatg ctctatcttt ctctgctggc
    80761 tgtcagctgt agcgtcagcg ccgcaaaata ccctgttctg acagaaagtt cgccagagaa
    80821 agcagggttt aacgtcgaac ggcttaatca gatggatcgc tggataagcc agcaagtgga
    80881 cgccggttat cccggcgtaa acctgctgat tataaaagat aatcagattg tttatcgcaa
    80941 agcctggggc gcggcgaaaa agtacgatgg cagcgcgtta atggcgcagc ctgtcaaagc
    81001 caccaccggg acgctgtatg acctggcttc caataccaag atgtacgcca ctaacttcgc
    81061 cctgcaaaag ctgatgagcg aaggcaaact gcatcctgac gatcggattg cgaaatatat
    81121 tccagggttt gccgacagcc cgaacgatgc gatcaaaggc aaaaacacgc tgcggatttc
    81181 tgatctgctg catcacagcg gcggtttccc ggcagatccg caatacccga ataaagcggt
    81241 cgcgggcgcg ttatattccc aggataaagg ccagacgctg gagatgatca agcgcacacc
    81301 gctggagtat caaccctgca gcaagcatat ctacagcgat gtcgattata tgctgcttgg
    81361 atttatcgtc gagtcggtta ccggtcagcc acttgaccgc tatgttgaag agtcgattta
    81421 tcgcccgctc ggcctgacgc acacggtatt taatccgctg ctgaaaggct ttaaaccaca
    81481 gcaaattgcc gccacggaac tgaacggcaa tacccgcgat ggcgtgattc acttcccgaa
    81541 tatccgcact tccactctct ggggtcaggt gcacgatgaa aaagcctttt attcgatggg
    81601 cggcgtttcc gggcacgcag gtttgttttc cgataccggc gatattgcgg tgttaatgca
    81661 aacgatgctg aacggcggtg gctatggtga tgtgcagctg ttcaatgcgg aaacggtgaa
    81721 gatgttcacc accagctcta aggaagatgc cacttttggc cttggctggc gcgtgaatgg
    81781 taatgccacc atgacgccga cgtttggcac gctggcaagc ccgcaaacct acggtcacac
    81841 tggctggacc gggacggtga ccgttatcga tccggtgaat catatggcga ttgtgatgtt
    81901 aagcaacaag ccacattcgc cagtagccga tccgcaaaag aatcccaata tgtttgaaag
    81961 cggtcagttg ccgattgcaa cttatggttg ggttgttgat caggtgtatg cggcgttaaa
    82021 gcagtaataa aaaggccagt cagttgtgat actggctggc ctttaatcat cgaatcagaa
    82081 acgcttacaa cgccattaac ttgtccagcg acggcgcgaa gtaatagccg ccagtgaccg
    82141 gtttggtaaa acgcaacatc gcatcacgtt tgccatcggt atcgccaaac atgctcagca
    82201 gttgctgctc aatgttatgc agacgcgcgc agtaggcgca gaagtacaga ccgtgcgtac
    82261 cgctggcggt gccgtacggc aggctctgtc gaacaatctt cagccctttg ccatcttctt
    82321 tcagatcaac gcgggtcagg tgagaggttt ccggacgttc gtcgccgtca atctcttcgt
    82381 tggcctcttt agtgcgcccg atcatcatct cttgatcgtg aatgctcatc cggttgagtt
    82441 gcttcaggtt gtgctcccaa cgctgcacaa acacatagct gccgccggca tccacgccat
    82501 ctttgataac cgccacttcg cggcgcgtct cttcacccgc cgggttttct gtaccgtcaa
    82561 caaagccgct caggtcacgc tcttcaaccc aacggaagcc gtggatctct tctttcactt
    82621 caatgcaatc accaaacgct tccatcgccg cctgggcgac agagaagttt acgtcgtgac
    82681 gcagagaaag aatgtggatc aacacatcga actgggtcgt cggtgcaaga cctttaccgt
    82741 agcccggaaa atctttcagc tcttcggccc caacgccgcc gctcagagcg cgccaggtat
    82801 tgttaccaaa ggcaaccacc gcaccaagat gcgcgtccgg gaatttcgct tcaaaagttg
    82861 ccagcttgtc ggcaaatgtt ttactggccg cacgcagggc gtcaacttcc cctttcacgt
    82921 tggcttcgat ccaaatcgcc gcgcggcaat gttctggcaa aatgccactc tgaacctgag
    82981 acattgttcc tcctgaaaat aagaatgcca cgcaatccgt ggcattgttg ggcgctattt
    83041 tacgtgtttt ttcgcggcgg cggtttgttc agacgcaaat taacgccgcc agataatttt
    83101 actgactttc cagtttttca gggtatcgtc cgaaggcatt aacccttcag gaccactcca
    83161 ttccccgctg aactggtagc taatatgttg actgccttcg gctttacatt cgacggcacg
    83221 attatcatca ccatcagctt tttggcaatt gccaaatgct ttgctgtaaa ggtcgctaaa
    83281 cggtgtaccg attttaacac cggtgtcagc aggaatatcg ctatccagca cgtcaatgcg
    83341 actgatcgtg ccttgatcgc cgttaatcac catcgccacg ttgtcgccct tcatcacttc
    83401 aaaaaagcgc accacgttgc cgttcgcggt tttcattccg ctgcgcaggc gataatcgcc
    83461 atcaagcgca tcggcaatgg cttgttcttg cagtggtgtg gacgccgtta attcaccaac
    83521 gccctgctcg ctcactttgg tggatgatcc aaaccagttc cacggattag cggcagacca
    83581 gttaacggac gacatcgtgg aacagccggt cagcatcaac ggcatagcgc ataacattaa
    83641 acgcagcgat ttcatgtcac ttcctttggt tattcaataa cgttgcttgg agtgcaaatt
    83701 caccaaaaag tgccgttatt ctttttcttc attaaaacaa gcgcgtaaac gtcgattggt
    83761 cagcagccag attaacgcca caatatccgc cactaccagc gccagaccga taccgctaac
    83821 ggattcaccg ttcagccaca accacggctg ccagcaaagt aaaaccactt gcgccaacag
    83881 caacagaaaa tagagcacat gccagatacg gggaaatgaa gcccgccgac cactcagcaa
    83941 aaacgccagc accgccggaa tgccgggaat caaccccagc cagaaattat cgtgatcggg
    84001 ataaaacaga tttagcagtg cagtaccctg ctcgcgcgac gcaccggcaa tgacaaacag
    84061 cacccaggtt cgcgcctgaa gcaatagcac aagccagaag agcaagggta aacgcaggcg
    84121 accgtacgca tcataatgga caggatgaaa ctcagtactc ttcatcttca atcaaacgct
    84181 tacccagact cagcacgtcg gcgtgttcat atcccaggcg ttcatacatt ccgagcacca
    84241 tgtcgttatc ttccggcacg ttgatctgaa ttttcgggca gccgcgagca atcagctttt
    84301 tctccaaccg attaagcaac gcattagcaa tcccacgccc acgaaactct ggatgcacgc
    84361 caagataata agcagacccg cgatgcccgt cataaccgcc catcaccgtt ccgaccactt
    84421 caccgttcac ctcagcgacc agaaacaaac tgacgtcatg gttcatctta cgctcaatgt
    84481 ccatttccgg atcgttccac ggacgcagca agtcgcaacg ctcccaaagg gtgatgacct
    84541 cttcgaaatc ttcctggcga aatacgcgta tctccatggt attcgttacc tttttgcggg
    84601 ttaaaaggct gattatggcg tgaacggtcg aattagccaa tatctgacga aaatcggttg
    84661 aaaaagtggc ataatgggga gttgtcaact attgaaatga aaagtaaaac aattctcaac
    84721 agcaaacggt cgtaacggat tacgcgatac gatataacat ctggaacttt attattacaa
    84781 ctcaggccgt atgagcactt ttaaaccact aaaaacactc acttcgcgcc gccaggtgct
    84841 gaaagcagga cttgcagcgc tgacgttgtc aggaatgtcg caagccatcg ccaaagaaga
    84901 acctttaaaa accagcaacg gacacagcaa gccgaaagca aaaaaatctg gtggcaaacg
    84961 tgtcgttgtt ctcgatccag gccacggcgg gattgatacc ggagcgatcg gacgcaacgg
    85021 ttcaaaagaa aaacatgtgg tgctggcaat tgccaaaaac gtccgttcca ttttgcgtaa
    85081 tcatggcatt gatgcgcgtt taacgcgttc tggcgatacg tttatcccac tgtacgatcg
    85141 cgttgaaatc gcccataaac atggcgcaga tctgtttatg tcaattcatg ccgacggctt
    85201 taccaacccg aaagctgccg gtgcttcggt atttgccctc tctaaccgtg gggcaagtag
    85261 cgcaatggcg aaatacctgt ctgaacgcga aaaccgcgcc gatgaagttg ccggtaaaaa
    85321 ggcgactgac aaggatcacc tattgcagca agtgctgttt gatctggtgc aaaccgatac
    85381 tattaaaaac agcctgacgc tcggctcgca tattctgaaa aagattaagc cggtgcataa
    85441 actgcacagc cgcaacaccg aacaagccgc gtttgtggtg ttgaaatcgc cgtcggttcc
    85501 ttcggtgctg gtggaaacct cgtttatcac caacccggaa gaagagcggc tgttaggcac
    85561 ggcggcattt cgccagaaaa tcgccacagc gattgccgaa ggcgtgatca gttatttcca
    85621 ctggtttgac aaccagaaag cacactcgag aaagcggtaa gtgatgaaac ccgacgcaca
    85681 ccaggttaaa cagtttctgc tcaaccttca ggatacaatt tgtcagcagt tgagcgccgt
    85741 cgatggcaca gaatttgtcg aagatagctg gcagcgcgaa gctggcggcg gcggacgtag
    85801 ccgggtgttg cgtaatggtg gtgttttcga acaggcgggt gtcaactttt cacatgtcca
    85861 cggtgaggcg atgcctgctt ccgccaccgc tcatcgcccg gaacttgccg ggcgcagttt
    85921 cgaggcgatg ggcgtttcac tggtagtgca tccgcataat ccgtatgttc ccaccagcca
    85981 cgcgaatgtg cgctttttta ttgccgaaaa accgggtgcc gaacccgtct ggtggtttgg
    86041 cggcggcttc gacttaaccc ctttctatgg ttttgaagac gatgctattc actggcaccg
    86101 caccgcccgt gacctgtgcc tgccatttgg cgaagacgtt tatccgcgtt acaaaaagtg
    86161 gtgcgacgac tacttctacc tcaaacatcg taacgaacaa cgcggcattg gcgggctgtt
    86221 ctttgatgat ctgaacacgc cagatttcga ccactgtttt gcctttatgc aggcggtagg
    86281 caaaggctac accgacgctt atttaccaat tgtagagcga cgtaaaacga tggcctacgg
    86341 cgagcgcgag cgcaatttcc agttatatcg tcgcggtcgt tatgtcgagt tcaatctggt
    86401 ctgggatcgt ggcacactgt ttggcctgca aaccggcggg cgcaccgagt ctatcctgat
    86461 gtcaatgccg ccgctggtac gctgggagta tgattatcag ccagaagatg gcagcccgga
    86521 agcggcgtta agtgagttta ttaaggtcag ggattgggtg taactccctc ccccccattc
    86581 ccgcatccgc tgatgcagcg tcagtgacgg cttctcggca aacagctgct ggtaatccgt
    86641 ggcgaattgc cccagatgcc agaatcccca ctgcatggcg gcgtctttta ccgttgtgct
    86701 ttgcgaccaa gggcttatca gttcgcggcg tacggcgttc aggcgaatgc gtttcagcca
    86761 cgcgtttggg ccaatgccta aaatagcgtg aaacgcgttt tgtagcgtgc ggcggctgac
    86821 atgcagttga ttacacaagt ccagcaccgt cactggttcg gacatgtttt ccagcacata
    86881 ttcacgggcg cgggaaagca atcgacggta actctgatga ctgatgcttt ccgccgtcac
    86941 cattggctgc gcttcttcca gcatcgcccc catcgccatt agcaaattat cccccagcac
    87001 ttttcgcact gcaggctgat ggagattttc cgggttttcg caaaacgtcg ccagcgcctg
    87061 ttggacaaag ccccacagag cggctttatg ctgctctttc acttccaacg ccgactggct
    87121 acgcagcata tgtaataccc gatccggatt atgcaaaaag ttagcctgac gagtgatgac
    87181 atcttcagaa agcactacgc caaggatcgt gtaatcgtcc ggcgtactta attcaaactc
    87241 ggttccaccg gggcgcgtcg ctatttctgc gcttcccaga cattgcgaac cgataaatcc
    87301 ctgctcaccg cgcgtcgccg gaatgccaaa ccagaacgag ttcggccaga ccaggcacga
    87361 ctggcgcagc gccagaccgg tgtattcacg gaaaacctga atatcatcga gtagaatttc
    87421 cgtaaattca ccatgaaact tgcccggatg cagctgatcg taaatctgct gccaggcggt
    87481 aattgtcaaa gcatgttcat agacatccgt tgtctgtcgt tgatgaacat tatccacttc
    87541 gaccttcggc gtgagcttca ggttttcggg taagggttca tgataaagat ggtgcaaatt
    87601 ggctgtacgg gtctttttca tgatgttaat gccgggtgtt gtaggacacc cggcacctcc
    87661 gacaggttaa tggggcttga gacgataacg actactgcgt ttacgtaacg tcccggcaga
    87721 aaagagctgt tcgagcgcat ttctggcttt ttccagcggc cagccaaagt gggcagccac
    87781 ttctcctgcc gtcattccct gacgtactga tgttaacagc gccaacaact cgtcagcaga
    87841 ttccgcgacg ataactggcg cgtcgggttc ctttacgggt tgcttaggct ggcggttaaa
    87901 gccagtaacc agccactggg tgtcatcgtc tggccgaccg atctccttac ggctgatgac
    87961 acgcccggta cgcatcgcgg cagcacaacc tgcgtccagc gccgcacgac acgccgccag
    88021 atcgccttcc accaccagcg ttaggcgacc agggtcaagc acttcgtgac tgagcagacg
    88081 aacgttagct gctttgagca tggcatcagc tgcatctatc gcggcgacca tgccgtccac
    88141 ttccagcaat cccagtgcat tgatcatcgg caacctccgt tacgcacgct ggattggatt
    88201 acgcgcgatt tccagcactg catcggtaaa ggcgttacag gctgctttac acgcggcctg
    88261 gctaccggtt aaaaatgctg ccgagtagtt ggtttcagac ggtggcggga cataggtcac
    88321 cagctgtacg tcggcagatt tcagggctgc atcaatgccg taggtcgctt ccagcggcgg
    88381 tgccaccaga tacgccatcg gatcgccaag cgtgatcccg gcggttgatg agagataaga
    88441 accggtacgc gaaactacat gtgccaggaa ggctgtgttt tctgcgtcat tagcccactg
    88501 gaaagcagcg ccattttcaa tatgcgcaac catcgcatcc agaccagcac gcacttccgc
    88561 cgggtttggc ccaccaagca taatcagcac ttcaccggca gtcggtgacg ggccgtgtgc
    88621 ggctcccgca tacagcgagc ggccatacac cacttccacc atcgcctgct tggtcgcttc
    88681 gtcagccgca atgtaggtga cgtcatctga atcagcagaa atcagcccga ggctacgaat
    88741 atgcggcggc aatttcagtt cacgcgcaaa ttcggcgtta acggaggcaa tcacccgcat
    88801 ggcggttacc gacggtcgaa tcaaatctaa agctggcatg atgcctcctt atcgggtcat
    88861 gttgatgccg gatgctttct gctccagcat acgtttcgcc aaatccacaa tgacggctgc
    88921 ggcttcaacc ggcggcgtgc cgccctggtg aatgttagag atacaggtac ggtcggcctc
    88981 aacggtggtt gccatacgcg gcgagtaaac ggcgtagcag gagaggcttt ctgactgccc
    89041 taatcccggg cgttccccca ccagtaggat cactactttc gcgccgagga tctcaccgat
    89101 ctgatcttca atcttcacgc gaccataacg aacaaagaat ggtgtaccga ctttcagccc
    89161 ggcctgtttc aggcccgcca tcagcggtgg caggatctct tcgtagttca cggtgatcgc
    89221 atcagttgac aggccatcag aaataacgac ctgtacgtcc ggattagcaa cacactgtgc
    89281 tttcagcgct tcaacagctt ctgcacacag gcggcggccc atatccggac gagtcaggta
    89341 gagatttttg tcgctaattt cagagcggac ttccagcaag ccctgcgctt tcacccactc
    89401 ttccggtact tctttcagta cggtatcttt cgaacgagag tgatcggcca ggaaacgcag
    89461 caacgcctgg gtacgcggac gcggaccggc acgaccggta caaacgcggg ccacggtgct
    89521 gcgacgcagt tctgttaata cgtctgcgcg atgcgggttt tccacaccaa tccacgcttt
    89581 tgcttccgca gaacctaaat ccagcgcgca gctttccgag gtcactggtg tcgtacaggt
    89641 ggtggtggcg cactttgctt ctgacggcgc gggggccgct tgtcccattg acgccatcac
    89701 gctgcgtaca atttcttcaa tctgtttttg atccatgatg tgttatctcc gcgtcatcag
    89761 aagaacagtg acggatcgcc cgcccgtttg gtcaggcgac cgtttgccat aatgcccatg
    89821 ctttccagcc agcgttcaaa ctccggtgac gggcgcaggt tgagtaactg acgcacagtg
    89881 gcggtgtcgt ggaaggccgt agtctggtag ttgagcatga tgtcatcacc cagcggcatc
    89941 cccatgatgt agttacagcc tgcggtggcg agcaggatca tcaggttttc gttgaggttc
    90001 tggtcagcgt cagcgtggtt ggtgtagcag cagtcacagc ccatagagat gccgctcagc
    90061 ttgcccataa agtgatcttc taagcccgcg cggataatct ggcggtcgtt gtagagatac
    90121 tccggcccaa taaaaccgac cacggtgttg acgataaacg gatcgtaatg acgcgccagc
    90181 ccatagttac gcgcttccat cgtcacctgg tcagcgccga agttagcgcc agcggacagc
    90241 gccgaacctt gtccggtttc gaagtagagg cagttttccc cggcgatacg gttaaactct
    90301 gcacccactg cgcgcgcttc gtcgagcatc gccagctcca cgccaaactc tttcagccct
    90361 ttttcgctgc cgcaaatact ctggaaaatc agcccgcccg gtgcgccgcg acgaatcgct
    90421 tcgatctggg tggtgacgtg cgccagtacg cagccctgag ttgggatgtt gaatttgtcg
    90481 atcacgccat agatggtatc caacacgcgg cttaagtttt ccacgtcgtc agtcaccggg
    90541 ttaacgccga tcaccgcatc gcccaccccg aaggaaagcc cttcgtagat ttgcgcggcg
    90601 atactttgca catcgtcacg ggtatcgttt ggctgcaagc gggcgctaaa ggtgcccgga
    90661 ataccgatgg tggtattggc ctttttgatc accggcattt tcttcgcgcc gtagatcagg
    90721 tccgcgttgg agcaaatctt cgctaccgcc gctaccactt ccgaggtcag ccctttgcgg
    90781 gtaaaggcaa tgtcgtccac gctggtttca tcgctcagca catactcacg cagttcgctg
    90841 atgctccagt ttttaatctg gttgtaggcc gtttcgttaa catcgtcctg aatcagccgc
    90901 gtcacgcagt catcttcata ggcaatcacc ggattattgc ggatgtccgc tacggtcatt
    90961 tccgacaaca cctgctttgc cgccacgcgc tcctgtgagc ttgccgcagc aacgcccgcc
    91021 agcacatccc ccgaacgcag ttcgttggct ttagccagca cctcttttac atccttaaac
    91081 tgatatacat tgccgaacaa tgtggtcttt agtttcataa gtcgttccct caggaaggaa
    91141 atgcgagtga tttcaccgtc accggcacaa ccgatccgcc aaaaagaggc gtaccaatgt
    91201 cgatatagtc ccccgcgcgg acaatcactt cgtcaatgac tgccaacggg agttgttgta
    91261 gctgtgggcg caacaacatg cccagagctt taccaaagtc ctgcccggcc accaccagca
    91321 ggggatgcgg attcggaaaa cgcgcgacga aatcgaccaa cgcgttgatg accgtcagta
    91381 ccgcggcgta acgcacaggc agcgaggcgg gaagcgccag cacgtacgcg tcagttttag
    91441 gatcaagatc cagctgaatc agcgcctgtt gccaggcact caccagatcc ttttcatcaa
    91501 ttgggatcgc caccggcaag ttgcgcagcg gcagttgtac gccctccagc cagattgtgc
    91561 tgccagagag cgaaagggta tgtgcacccg cgccaatcac cgtggcgcgc acggtttgcg
    91621 ccggaaactg cacgttcatc tcacgcaggc gcggatggtc atgcagcgcc gttgccagaa
    91681 gcgggccaat atcggcaaaa cagaacgggt cggcgggctg gtggcgataa cattcgccaa
    91741 cgccgccaga aagcgtaatg atttcgggcg taacacctac gggcagcaaa ccggtttgca
    91801 tcaatgcctg cgcgagcggc gaaagcgttc cgtcaatcac ttcgacaatc agctctgcca
    91861 tccgccgggt aacctgcacc agctgcgcgc cggtcagcga acgggcttcg gtgcctgcac
    91921 cgaagcactc atccacaatc atctgccccg gtttatgagc gtaaaccaca cgcccctggc
    91981 tgtcggtttc cagcaggcga ccgccgacat tgaggcaggc ggtgccgctg atttttccgg
    92041 catcgaacag ggcgtagttc gcggtgccac cgccgatatc gatattcagg acccgacaca
    92101 gccgttgttc agaaagggtt tgcgccccgg caccgtgacc ggcgatcacg gattcgagat
    92161 gcggtccagc gctggcgacg acaaaatcgc ccagcgactg ggagagcgtc atcaccgccg
    92221 ggcgagcgtt gcgggttttc gcgctttcac cggtgatgat gatggcacca gaatcaacgc
    92281 tttccggcgc aatgcccgca gcctgatatt gctcgaggat taaggttttc agttccgctt
    92341 cttttaaacc accctgttta tcgacagggg taaagaacac cgggctttgc cagctaattt
    92401 cgcgtttgat gaattcgtag cgcggcacct gcgacaccgc cgcacggtta accagctcca
    92461 ggtgggagaa aatcacctgg gtggtggtgg tgccgatatc gataccgacg ctcagtagct
    92521 ggcgagtgtt cacgattgcg cctccgcttc ggttttagcc gcgctcgcgt cttctttagg
    92581 taccagcatc atcgccacgc caatcgccgt gacgccgccg atcagcttgc cgacaatcat
    92641 cgggaagatc atggcgttca tgttggcagc cgcgaagcct aaatggtcgc ccagggcgaa
    92701 agcagcggaa acggcgaagg cacagttgat gactttgccg cgggtatcca tctgcttcat
    92761 catgccaaac atcgggatgt tgttggcaag cgttgccacc atgccggctg ccgctatatt
    92821 gttcatattc agcaccttac cgacactcat cagcggtttt tcaaaccagc gagtcagcag
    92881 cagcaccatc ggatacgccc ccagcagcac gcaggagata gaaccgataa cttcaatggc
    92941 gcgcatcact tcaccgggtt tgtcgcctgg agccatgaag atcggatcca gaccggggat
    93001 cagttcccaa ccgagcagga atttcactac cgcagcggca agaccgaggg tgatcaatgc
    93061 aacgaggaat ttggcgaaga tctggaagcc gttgatcatt ttttccggga tgaatttcag
    93121 ccccagcgcc accagcaccg caacgataag taccgggatc atgttcatca ggatcagcgc
    93181 aaaggtgaat tccaccggct gaccgttgat ctgcacaccg gagtacatag caaccagacc
    93241 accagcaata cagccaatcg gaatggtcac aatgcccgcc agcacgccga gcgccagata
    93301 acgacggtca gaaggttcga taatgccgag cgccaccgga atggaaaaca caatcgttgg
    93361 ccccatcatc gacccgagaa ttaacccaga gtatagccac gctgctacgt cgccgcccgc
    93421 cagctctttg gcgaggaaga agccgcccat atcgcacgcc agcagtgttc cggcgaacat
    93481 cgatgggtta gcgccgagca tttcgtaaac cgggataatc accggcccga gtacgtgggc
    93541 cagtaccggt gccagcgcgg tcataccgac catagccagg cccaaagcgc ccattgccat
    93601 aaagccttct tcgaactgac cgccggatcc ttcgatactt ttaccgaact taccgaggaa
    93661 acgagcagaa ccgccgaact gcgacaggat cctgtctacg gcagctatca gcataaagaa
    93721 catcatgatg tacatgatga tttcgttaat acccatcgcc tttactccct gttagttgtt
    93781 atttattggc ggatgcggcg tgaacgcctt atccgcccta catgtgcaat cccgaaggcc
    93841 agataagacg cggcaagcgt cgcatccggc aattgcaccg cgccactggc ggatgcggcg
    93901 tgaacgcctt atccgcccta catgtgcaat cccgtaggcc ggataagacg cggcaagcgt
    93961 cgcatccggc atttattgcg ccgctgcgta caggccgaca atctgctcca ggctggcggt
    94021 acgcgggtta ctgcgcagac aaatatcttc cagcgcggcc tgcgcccatg cgccgtaatg
    94081 cgcagatgtc gcaccaacat cgcccagtcg tttaccaatc ccaacttccg ctatcagctc
    94141 acttaccgca ttaatagcgt cacgatcgtc ggatttttta gttcgcagcg cccgaccaac
    94201 caagctaaac cgatcacgac aaaccatccg gttaaattcc atcaccgttg gcaacaacat
    94261 ggcgttcgcc agaccgtgcg gaatatgcag cgccgcccct ggctgatgcg ccatcgcgtg
    94321 gcacaaccca agtccggcac tggaaaacgc cattcccgcc atacatgaag ccagcaacat
    94381 gctctcgcgc gcggcaaggt catgaccgta gcccaccgct ttcggcagcg atttgccaat
    94441 catcgcaatg gcaccaatcg ccagactgtc ggtaaacggt gtggcgttca gcgcgctgta
    94501 cgcttcaatg gcatgggtta acgcatcaat gccagtcatc gccgtgacat gcgacggcac
    94561 accttcggtc aatgcggcgt cgagaatcgc cacatccggc atcagcgagg catgggctaa
    94621 cacctgcttg cgcccgctca ccgcgtcgat aatcaccgtt acgttggtgg tttcagagcc
    94681 ggttccggcg gtcgttggaa tggcaatcaa cggcaggcgc ggttgcagaa cgctggtttc
    94741 tgacatttct gccagcgtgc tatcggggtt cgtcaccagc aacgccacgg ctttcgccgc
    94801 atccagcacc gagccgccgc caaacgcgat caccccgtca caacctgatt cacgcaactg
    94861 cgccacggct gcacacacgt cggtgatgca cggttcgccc accggacatg gccagagcgt
    94921 catggcgata cctttaacgg ccaggctacg cgtcagcccg gcggtcatcc ccgcctgatg
    94981 caaaaagctg tctgccatca cgaacagatg tttcagccca cgcgtttgcg cttgctgccc
    95041 acaactgctc accgcgcccg gaccgcaaag cgtcaccggt ggaacgctaa atgttttcac
    95101 ccgctgcaga ttcagggtat cgaatgcctg aaaaagcgcc gtttgcaatt cactttgcat
    95161 agatcccctc cgttttctct cgcccactac tggcgatcgc cagcggggtc ataaacaaac
    95221 tgtgctgcgg taaatgcacc tgtaacgccg ggaattgttt gcgaaacagt tccgctacgc
    95281 ccggttgcat acaggaaccg cccgccagcc ataaatccgt aatgccctgc ccttcaatat
    95341 gccgggccac aatgtccgcc attttttcgt acaccggttt caccgcgggc caaatctcgt
    95401 caccgtgacc gcgtttgtac tgctctgcct cttccagcga aatacggcga tttccggcga
    95461 gagtcagaga gatgtgatgt ccgccggtgg cttcatccgc cgagtacgtc actttgccct
    95521 ttttcacgat ggcaatgccg gtagtgccgc cgccgatatc cactacaccg gcgttgtcca
    95581 gttgcagcag atccgcaact gcggttggct catccagcac atggctcact tccagtccag
    95641 cagactccag cacgttgatg gaaatacgcg ggtccgtacc tggaggaaaa gaggtcgccg
    95701 catggctaaa acggcgaccg aattgctgct cgagggtgtc gagatggcga cgaacaatgg
    95761 tgaccgcacc gaagaaatcc cagacgatgc cgtcgcggac cacatcagcc cagtcgaggc
    95821 ataccgccac cggctgaccg tcgcggtcga caaccatcga caccacatcg caggtgccca
    95881 gatccacccc cagccacagc ggagattccg tcgcggcggg cgtctggtta cacagcgttg
    95941 ccgccgtttg cagacgtggg gtgagccatt gttcgtcgtg cgccatctgt tactccttaa
    96001 acaatgcgaa acgcatcgac taatacacag cgacgcagac ggacaaacgt ccgcgcgctg
    96061 gttacccctt caccggttgg cgtggtgatg gtcatagtgg t
//
LOCUS       DAZGHF010000004        83689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGHF010000004 DAZGHF010000000
VERSION     DAZGHF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.004"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            356..982
                     /gene="ydhY"
                     /locus_tag="ACHK3A_000310"
     CDS             356..982
                     /gene="ydhY"
                     /locus_tag="ACHK3A_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO8738455.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTHRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1003..3105
                     /gene="ydhV"
                     /locus_tag="ACHK3A_000311"
     CDS             1003..3105
                     /gene="ydhV"
                     /locus_tag="ACHK3A_000311"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO8738456.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRKTLQR
                     LGLEDIAADLAAHNLLPA"
     gene            3118..3756
                     /gene="ydhW"
                     /locus_tag="ACHK3A_000312"
     CDS             3118..3756
                     /gene="ydhW"
                     /locus_tag="ACHK3A_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO8738457.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDHADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3820..4488
                     /gene="ydhX"
                     /locus_tag="ACHK3A_000313"
     CDS             3820..4488
                     /gene="ydhX"
                     /locus_tag="ACHK3A_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO8738458.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTMQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4485..5270
                     /gene="phsC"
                     /locus_tag="ACHK3A_000314"
     CDS             4485..5270
                     /gene="phsC"
                     /locus_tag="ACHK3A_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO8738459.1"
                     /translation="MNPSQHAEQFQSQLANYVPLFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSANDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     TVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5274..6086
                     /gene="ydhT"
                     /locus_tag="ACHK3A_000315"
     CDS             5274..6086
                     /gene="ydhT"
                     /locus_tag="ACHK3A_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO8738460.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(6092..7702)
                     /gene="ydhS"
                     /locus_tag="ACHK3A_000316"
     CDS             complement(6092..7702)
                     /gene="ydhS"
                     /locus_tag="ACHK3A_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO8738461.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEEIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAIAQAVVKPASRARRRLSFNQM"
     gene            complement(7828..8133)
                     /gene="ydhR"
                     /locus_tag="ACHK3A_000317"
     CDS             complement(7828..8133)
                     /gene="ydhR"
                     /locus_tag="ACHK3A_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO8738462.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTNEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8241..8317)
                     /locus_tag="ACHK3A_000318"
     tRNA            complement(8241..8317)
                     /locus_tag="ACHK3A_000318"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8281..8283),aa:Val,seq:gac)
     gene            complement(8322..8398)
                     /locus_tag="ACHK3A_000319"
     tRNA            complement(8322..8398)
                     /locus_tag="ACHK3A_000319"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8362..8364),aa:Val,seq:gac)
     gene            8707..9963
                     /gene="ydhQ"
                     /locus_tag="ACHK3A_000320"
     CDS             8707..9963
                     /gene="ydhQ"
                     /locus_tag="ACHK3A_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO8738463.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSTYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGSINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10004..11377)
                     /gene="mdtK"
                     /locus_tag="ACHK3A_000321"
     CDS             complement(10004..11377)
                     /gene="mdtK"
                     /locus_tag="ACHK3A_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO8738464.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            11592..12233
                     /gene="ribE"
                     /locus_tag="ACHK3A_000322"
     CDS             11592..12233
                     /gene="ribE"
                     /locus_tag="ACHK3A_000322"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO8738465.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12273..13421)
                     /gene="cfa"
                     /locus_tag="ACHK3A_000323"
     CDS             complement(12273..13421)
                     /gene="cfa"
                     /locus_tag="ACHK3A_000323"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO8738466.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13712..14923)
                     /gene="punC"
                     /locus_tag="ACHK3A_000324"
     CDS             complement(13712..14923)
                     /gene="punC"
                     /locus_tag="ACHK3A_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO8738467.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            15036..15968
                     /gene="punR"
                     /locus_tag="ACHK3A_000325"
     CDS             15036..15968
                     /gene="punR"
                     /locus_tag="ACHK3A_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO8738468.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15965..16990)
                     /gene="purR"
                     /locus_tag="ACHK3A_000326"
     CDS             complement(15965..16990)
                     /gene="purR"
                     /locus_tag="ACHK3A_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO8738469.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17289..17378
                     /gene="cydH"
                     /locus_tag="ACHK3A_000327"
                     /gene_synonym="ynhF"
     CDS             17289..17378
                     /gene="cydH"
                     /locus_tag="ACHK3A_000327"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO8738470.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVVVGLTAALH"
     gene            17544..18713
                     /gene="ydhP"
                     /locus_tag="ACHK3A_000328"
     CDS             17544..18713
                     /gene="ydhP"
                     /locus_tag="ACHK3A_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8738471.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18859..19440)
                     /gene="sodB"
                     /locus_tag="ACHK3A_000329"
     CDS             complement(18859..19440)
                     /gene="sodB"
                     /locus_tag="ACHK3A_000329"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO8738472.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19568..20395)
                     /gene="mepH"
                     /locus_tag="ACHK3A_000330"
     CDS             complement(19568..20395)
                     /gene="mepH"
                     /locus_tag="ACHK3A_000330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO8738473.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASANFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20730..21077
                     /gene="grxD"
                     /locus_tag="ACHK3A_000331"
     CDS             20730..21077
                     /gene="grxD"
                     /locus_tag="ACHK3A_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO8738474.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21128..25744)
                     /gene="lhr"
                     /locus_tag="ACHK3A_000332"
     CDS             complement(21128..25744)
                     /gene="lhr"
                     /locus_tag="ACHK3A_000332"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO8738475.1"
                     /translation="MADNLNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSMERLDTLLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQADEWYSRVRRAAPWKDLPRRVFDVTLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARTLLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTSEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEANQANSSALQQAIVTVLADGGAWFAQQISQRIRDKIGESVDPSAMQEAL
                     WALVWQGVITSDIWAPLRALTRSSTNTRTSTRRSHRARRGRPVYAQPVSPLVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANAWGNL
                     LPWPAHPATLIPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEIFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25837..26484)
                     /gene="rnt"
                     /locus_tag="ACHK3A_000333"
     CDS             complement(25837..26484)
                     /gene="rnt"
                     /locus_tag="ACHK3A_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO8738476.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26587..26994)
                     /gene="gloA"
                     /locus_tag="ACHK3A_000334"
     CDS             complement(26587..26994)
                     /gene="gloA"
                     /locus_tag="ACHK3A_000334"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO8738477.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(27075..28172)
                     /gene="nemA"
                     /locus_tag="ACHK3A_000335"
     CDS             complement(27075..28172)
                     /gene="nemA"
                     /locus_tag="ACHK3A_000335"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO8738478.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28209..28808)
                     /gene="nemR"
                     /locus_tag="ACHK3A_000336"
     CDS             complement(28209..28808)
                     /gene="nemR"
                     /locus_tag="ACHK3A_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO8738479.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATPAV"
     gene            28911..29150
                     /gene="ydhL"
                     /locus_tag="ACHK3A_000337"
     CDS             28911..29150
                     /gene="ydhL"
                     /locus_tag="ACHK3A_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO8738480.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29199..30095
                     /gene="ydhF"
                     /locus_tag="ACHK3A_000338"
     CDS             29199..30095
                     /gene="ydhF"
                     /locus_tag="ACHK3A_000338"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO8738481.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDYLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30176..30697
                     /gene="sodC"
                     /locus_tag="ACHK3A_000339"
     CDS             30176..30697
                     /gene="sodC"
                     /locus_tag="ACHK3A_000339"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO8738482.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30698..32710)
                     /gene="ydhK"
                     /locus_tag="ACHK3A_000340"
     CDS             complement(30698..32710)
                     /gene="ydhK"
                     /locus_tag="ACHK3A_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO8738483.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELQHL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGSNALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32710..33567)
                     /gene="ydhJ"
                     /locus_tag="ACHK3A_000341"
     CDS             complement(32710..33567)
                     /gene="ydhJ"
                     /locus_tag="ACHK3A_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO8738484.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSNLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33570..33806)
                     /gene="ydhI"
                     /locus_tag="ACHK3A_000342"
     CDS             complement(33570..33806)
                     /gene="ydhI"
                     /locus_tag="ACHK3A_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO8738485.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLSLAILIAW"
     gene            34007..34441
                     /gene="slyA"
                     /locus_tag="ACHK3A_000343"
     CDS             34007..34441
                     /gene="slyA"
                     /locus_tag="ACHK3A_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO8738486.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(34488..34955)
                     /gene="slyB"
                     /locus_tag="ACHK3A_000344"
     CDS             complement(34488..34955)
                     /gene="slyB"
                     /locus_tag="ACHK3A_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO8738487.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35229..36338
                     /gene="anmK"
                     /locus_tag="ACHK3A_000345"
     CDS             35229..36338
                     /gene="anmK"
                     /locus_tag="ACHK3A_000345"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO8738488.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLILSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36436..36765
                     /gene="mliC"
                     /locus_tag="ACHK3A_000346"
     CDS             36436..36765
                     /gene="mliC"
                     /locus_tag="ACHK3A_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO8738489.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLFERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36824..37480
                     /gene="pdxH"
                     /locus_tag="ACHK3A_000347"
     CDS             36824..37480
                     /gene="pdxH"
                     /locus_tag="ACHK3A_000347"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO8738490.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37609..38883
                     /gene="tyrS"
                     /locus_tag="ACHK3A_000348"
     CDS             37609..38883
                     /gene="tyrS"
                     /locus_tag="ACHK3A_000348"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO8738491.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38942..39805
                     /gene="pdxY"
                     /locus_tag="ACHK3A_000349"
     CDS             38942..39805
                     /gene="pdxY"
                     /locus_tag="ACHK3A_000349"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO8738492.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39849..40454)
                     /gene="gstA"
                     /locus_tag="ACHK3A_000350"
     CDS             complement(39849..40454)
                     /gene="gstA"
                     /locus_tag="ACHK3A_000350"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO8738493.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40560..42062)
                     /gene="dtpA"
                     /locus_tag="ACHK3A_000351"
     CDS             complement(40560..42062)
                     /gene="dtpA"
                     /locus_tag="ACHK3A_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO8738494.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42673..43308)
                     /gene="nth"
                     /locus_tag="ACHK3A_000352"
     CDS             complement(42673..43308)
                     /gene="nth"
                     /locus_tag="ACHK3A_000352"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO8738495.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43308..44003)
                     /gene="rsxE"
                     /locus_tag="ACHK3A_000353"
     CDS             complement(43308..44003)
                     /gene="rsxE"
                     /locus_tag="ACHK3A_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO8738496.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(44007..44627)
                     /gene="rsxG"
                     /locus_tag="ACHK3A_000354"
     CDS             complement(44007..44627)
                     /gene="rsxG"
                     /locus_tag="ACHK3A_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO8738497.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44631..45689)
                     /gene="rsxD"
                     /locus_tag="ACHK3A_000355"
     CDS             complement(44631..45689)
                     /gene="rsxD"
                     /locus_tag="ACHK3A_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO8738498.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGV
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45690..47912)
                     /gene="rsxC"
                     /locus_tag="ACHK3A_000356"
     CDS             complement(45690..47912)
                     /gene="rsxC"
                     /locus_tag="ACHK3A_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO8738499.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKACKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(47905..48483)
                     /gene="rsxB"
                     /locus_tag="ACHK3A_000357"
     CDS             complement(47905..48483)
                     /gene="rsxB"
                     /locus_tag="ACHK3A_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO8738500.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISYNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48483..49064)
                     /gene="rsxA"
                     /locus_tag="ACHK3A_000358"
     CDS             complement(48483..49064)
                     /gene="rsxA"
                     /locus_tag="ACHK3A_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO8738501.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFFQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(49141..49581)
                     /gene="ydgK"
                     /locus_tag="ACHK3A_000359"
     CDS             complement(49141..49581)
                     /gene="ydgK"
                     /locus_tag="ACHK3A_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO8738502.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49667..49882)
                     /gene="ydgT"
                     /locus_tag="ACHK3A_000360"
     CDS             complement(49667..49882)
                     /gene="ydgT"
                     /locus_tag="ACHK3A_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO8738503.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(50155..50280)
                     /gene="blr"
                     /locus_tag="ACHK3A_000361"
     CDS             complement(50155..50280)
                     /gene="blr"
                     /locus_tag="ACHK3A_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO8738504.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            50523..51563
                     /gene="ydgJ"
                     /locus_tag="ACHK3A_000362"
     CDS             50523..51563
                     /gene="ydgJ"
                     /locus_tag="ACHK3A_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO8738505.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEGWGYDMRD
                     GVLTRVEGEERIEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51599..52600)
                     /gene="add"
                     /locus_tag="ACHK3A_000363"
     CDS             complement(51599..52600)
                     /gene="add"
                     /locus_tag="ACHK3A_000363"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO8738506.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(52704..53876)
                     /gene="malY"
                     /locus_tag="ACHK3A_000364"
     CDS             complement(52704..53876)
                     /gene="malY"
                     /locus_tag="ACHK3A_000364"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO8738507.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFASAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSEKGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKRADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53886..55478)
                     /gene="malX"
                     /locus_tag="ACHK3A_000365"
     CDS             complement(53886..55478)
                     /gene="malX"
                     /locus_tag="ACHK3A_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO8738508.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55653..56681
                     /gene="malI"
                     /locus_tag="ACHK3A_000366"
     CDS             55653..56681
                     /gene="malI"
                     /locus_tag="ACHK3A_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO8738509.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECSSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56793..57560
                     /gene="hdhA"
                     /locus_tag="ACHK3A_000367"
     CDS             56793..57560
                     /gene="hdhA"
                     /locus_tag="ACHK3A_000367"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO8738510.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57781..58371
                     /gene="uidR"
                     /locus_tag="ACHK3A_000368"
     CDS             57781..58371
                     /gene="uidR"
                     /locus_tag="ACHK3A_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO8738511.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLSREASFAQGLRAMIGGILTAS"
     gene            58758..60569
                     /gene="uidA"
                     /locus_tag="ACHK3A_000369"
     CDS             58758..60569
                     /gene="uidA"
                     /locus_tag="ACHK3A_000369"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO8738512.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQEIRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            60566..61939
                     /gene="uidB"
                     /locus_tag="ACHK3A_000370"
     CDS             60566..61939
                     /gene="uidB"
                     /locus_tag="ACHK3A_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO8738513.1"
                     /translation="MNQQLSWRAIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LINDITS"
     gene            61978..63243
                     /gene="uidC"
                     /locus_tag="ACHK3A_000371"
     CDS             61978..63243
                     /gene="uidC"
                     /locus_tag="ACHK3A_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO8738514.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQPADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(63288..64796)
                     /gene="ydgA"
                     /locus_tag="ACHK3A_000372"
     CDS             complement(63288..64796)
                     /gene="ydgA"
                     /locus_tag="ACHK3A_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO8738515.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(64897..66072)
                     /gene="manA"
                     /locus_tag="ACHK3A_000373"
     CDS             complement(64897..66072)
                     /gene="manA"
                     /locus_tag="ACHK3A_000373"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO8738516.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISECYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVRGHGRLARVYNKL"
     gene            66271..67917
                     /gene="fumB"
                     /locus_tag="ACHK3A_000374"
     CDS             66271..67917
                     /gene="fumB"
                     /locus_tag="ACHK3A_000374"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO8738517.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            68060..69463
                     /gene="fumC"
                     /locus_tag="ACHK3A_000375"
     CDS             68060..69463
                     /gene="fumC"
                     /locus_tag="ACHK3A_000375"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO8738518.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRVFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGG"
     gene            complement(69460..70389)
                     /gene="tus"
                     /locus_tag="ACHK3A_000376"
     CDS             complement(69460..70389)
                     /gene="tus"
                     /locus_tag="ACHK3A_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO8738519.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKTAVRLPGVLCYQV
                     DNLSQAALVSHIQHINQLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(70465..71766)
                     /gene="rstB"
                     /locus_tag="ACHK3A_000377"
     CDS             complement(70465..71766)
                     /gene="rstB"
                     /locus_tag="ACHK3A_000377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO8738520.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAVESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(71770..72489)
                     /gene="rstA"
                     /locus_tag="ACHK3A_000378"
     CDS             complement(71770..72489)
                     /gene="rstA"
                     /locus_tag="ACHK3A_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO8738521.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILQE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            72618..72953
                     /gene="ydgC"
                     /locus_tag="ACHK3A_000379"
     CDS             72618..72953
                     /gene="ydgC"
                     /locus_tag="ACHK3A_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO8738522.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(72950..73672)
                     /gene="folM"
                     /locus_tag="ACHK3A_000380"
     CDS             complement(72950..73672)
                     /gene="folM"
                     /locus_tag="ACHK3A_000380"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO8738523.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIISYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(73709..75091)
                     /gene="ydgI"
                     /locus_tag="ACHK3A_000381"
     CDS             complement(73709..75091)
                     /gene="ydgI"
                     /locus_tag="ACHK3A_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8738524.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWS
                     ITGAGILLLTFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(75277..76221)
                     /gene="ydgH"
                     /locus_tag="ACHK3A_000382"
     CDS             complement(75277..76221)
                     /gene="ydgH"
                     /locus_tag="ACHK3A_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO8738525.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            76745..78277
                     /gene="pntA"
                     /locus_tag="ACHK3A_000383"
     CDS             76745..78277
                     /gene="pntA"
                     /locus_tag="ACHK3A_000383"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO8738526.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFTAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            78288..79676
                     /gene="pntB"
                     /locus_tag="ACHK3A_000384"
     CDS             78288..79676
                     /gene="pntB"
                     /locus_tag="ACHK3A_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO8738527.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATILGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(79701..80735)
                     /gene="tqsA"
                     /locus_tag="ACHK3A_000385"
     CDS             complement(79701..80735)
                     /gene="tqsA"
                     /locus_tag="ACHK3A_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO8738528.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLQQYRNSIM
                     TPLKAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            81146..81511
                     /gene="mdtJ"
                     /locus_tag="ACHK3A_000386"
     CDS             81146..81511
                     /gene="mdtJ"
                     /locus_tag="ACHK3A_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO8738529.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            81498..81827
                     /gene="mdtI"
                     /locus_tag="ACHK3A_000387"
     CDS             81498..81827
                     /gene="mdtI"
                     /locus_tag="ACHK3A_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO8738530.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(81866..82687)
                     /gene="ydgD"
                     /locus_tag="ACHK3A_000388"
     CDS             complement(81866..82687)
                     /gene="ydgD"
                     /locus_tag="ACHK3A_000388"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO8738531.1"
                     /translation="MRRTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(82787..82870)
                     /locus_tag="ACHK3A_000389"
     CDS             complement(82787..82870)
                     /locus_tag="ACHK3A_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738532.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(82963..83271)
                     /gene="asr"
                     /locus_tag="ACHK3A_000390"
     CDS             complement(82963..83271)
                     /gene="asr"
                     /locus_tag="ACHK3A_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO8738533.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            83542..83646
                     /locus_tag="ACHK3A_000391"
     CDS             83542..83646
                     /locus_tag="ACHK3A_000391"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO8738534.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
BASE COUNT        21035 a        20517 c        21745 g        20392 t
ORIGIN      
        1 ggtgtgtatt ttaatttata gcgataaagt tatttttatt ttctgcgatg aacttcacaa
       61 ataattgtcg gttaagttaa atttatttca aatgataata aatatcagtt cacttataat
      121 ttcttgcttt gtgattttaa ttgataacga tcaatgttaa gagcccagaa taatcacatt
      181 attttttctc ctaaccataa aggattagtt tatcagcgtt aaatcttttc tcatacacta
      241 ttgggttagg gaaaatatct ggtgttgagg acagagtcag cagcgtatta gttgagtgcc
      301 taatatcagg ctgctgaatt gtcagcactg tgtttgcgaa gaggatgcga aataaatgaa
      361 cccggttgat cgtccactat taaatattgg cttaacgcga ttagaattct tgcgtatatc
      421 aggaaaaggc cttgctggtt taacaattgc ccctgcgtta ctgtcacttt tgggttgcaa
      481 acaggaagat attgatagcg gcacagtagg cttgataaat acacccaaag gggtgctggt
      541 gacccatcgt gcgcgatgca cgggttgcca ccgctgtgaa atctcttgta ccaacttcaa
      601 cgatggctca gtagggacat tcttctcccg tatcaaaatc catcgcaatt atttctttgg
      661 cgataacggg gttggctctg gtggcggcct gtatggcgat ctcaactata ccgcggacac
      721 ctgccgtcaa tgcaaagagc cgcaatgcat gaacgtctgc ccgattggtg cgattacctg
      781 gcagcagaaa gaaggctgta ttaccgtcga tcataaacgt tgtattggct gtagcgcctg
      841 tactacggca tgcccgtgga tgatggccac cgtaaatacc gaaagtaaaa aatcctcgaa
      901 atgtgtgtta tgcggtgaat gcgcaaacgc ctgcccaaca ggggcgttaa aaattatcga
      961 gtggaaagat attactgtgt gaatcttgca aaggaaaaaa tcatggctaa cggttggaca
     1021 ggtaatatat taagagtcaa tctcacgaca ggaaatatta ccctcgaaga ttccagtaag
     1081 tttaaaagtt ttgtcggtgg catgggcttc ggctacaaaa ttatgtatga cgaagtaccg
     1141 ccaggcacga aacctttcga tgaagcgaat aaattagttt ttgctaccgg cccattaact
     1201 ggatctggtg ccccctgtag ttctcgcgta aatatcacct cactttctac ttttaccaaa
     1261 ggaaatttag tcgtcgatgc ccatatgggg ggcttttttg cagcgcaaat gaaattcgct
     1321 ggatacgacg tcattattat cgaagggaaa gcgaaatcac cggtatggct gaagattaaa
     1381 gatgacaaag ttagccttga aaaagccgat ttcttatggg gaaaagggac gcgcgcaacg
     1441 acggaagaaa tttgtcgctt gaccagtccg gaaacctgtg tggcggctat tggtcaggct
     1501 ggggaaaacc ttgttcctct ctctggcatg ttgaatagcc gtaaccacag cggtggtgcg
     1561 ggaactggcg caataatggg ttcgaaaaac ctgaaagcga ttgcggttga agggacgaaa
     1621 ggggtcaaca ttgccgatcg tcaggagatg aagcgtctca acgattacat gatgactgaa
     1681 cttatcggtg cgaataacaa ccatgtcgtg ccaagtacgc cgcaatcgtg ggcagagtat
     1741 tcagatccca agtcacgttg gactgcacgt aaagggttgt tttggggcgc ggctgaaggt
     1801 ggtccgattg aaacgggtga gattccgcca ggcaatcaga acacggtcgg ctttcgtacc
     1861 tataaatccg tttttgactt aggtccggcg gcagagaaat acacagtaaa aatgagtggc
     1921 tgccactctt gcccgatccg ttgtatgacc caaatgaata ttcctcgggt gaaagagttt
     1981 ggcgtgccca gcacaggtgg taacacttgt gtagcaaact ttgtccatac caccatcttc
     2041 ccgaacggtc cgaaagattt tgaagataaa gatgatggtc gtgtgattgg taacctggtt
     2101 ggtctgaatc tgttcgatga ctacggccta tggtgtaact atggtcagct tcatcgcgac
     2161 tttacctatt gttacagcaa aggggtgttc aagcgtgttc tgcctgctga agagtatgcg
     2221 gaaattcgct gggatcaact ggaagcgggt gacgttaact tcattaaaga tttttactac
     2281 cgtctggcgc atcgcgtggg tgagctgagt cacctggctg atggttcata tgccatcgca
     2341 gaacgctgga atttgggtga agagtactgg ggctacgcga aaaataaact ctggtcgccg
     2401 tttggctatc cagttcacca cgccaatgaa gcgtcagcgc aggtcggctc cattgttaac
     2461 tgtatgttca accgtgactg catgacgcat acccatatca actttattgg ttccggcttg
     2521 ccattgaaac tgcaacgtga agtggcgaaa gaactttttg gttctgaaga tgcttacgat
     2581 gaaaccaaaa actacacgcc aatcaatgac gcaaaaatta aatatgctaa gtggtcgctg
     2641 ttgcgggtct gtttgcataa cgccgtcacc ctgtgcaact gggtctggcc aatgaccgtt
     2701 tcgccgctga aaagccgtaa ttatcggggc gatctggcgc ttgaagccaa attcttcaaa
     2761 gcgatcactg gcgaagatat gactcaggaa aaattagatt tagctgcaga gcgtatcttt
     2821 acgttgcatc gcgcctacac ggtaaaactg atgcaaacta aagatatgcg taatgaacac
     2881 gatcttatct gttcctgggt attcgacaag gatccgcaga tcccggtctt tactgaaggt
     2941 acagacaaaa tggaccgtga cgatatgcat gcttcgctca cgatgttcta caaagaaatg
     3001 ggctgggacc cacagcttgg ttgtccgacc cgcaaaacat tgcagcgtct ggggctggaa
     3061 gatattgccg ccgatctggc agcacacaat ctactgcctg cgtaaggaat gggtgagatg
     3121 aaccatcagg atgaattgcc gttagcgaaa gtcagcgaag ttgatgaggc gaagcggcaa
     3181 tggttgcagg ggatgcgtca ccccgttgat acggtaaccg aaccagaacc tgctgaaatc
     3241 ctggcagagt tcattcgtca gcattctgcg gctgggcagc tggtggcgcg cgccgtgttt
     3301 ctctcgccgc cctattcggt tgcagaagag gaactgtcag ttttgctgga aaacattaag
     3361 cagaacggag accacgcgga tatcgcgtgt atgacgggaa gccaggatga ttactattac
     3421 tcaacgcagg ccatgagtga aaactacgcg gcgatgtctt tgcaagttgt ggagcaggac
     3481 atttgtcgcg ccattgcaca cgccgttcgt tttgagtgcc agacgtatcc tcgtccttac
     3541 aaagtcgcca tgctgatgca agcgccatat tacttccagg aagcgcagat cgaagccgcg
     3601 atcgccgcga tggatattgc acctgaatat gctgatatac ggcaggtgga gtcatcgacg
     3661 gcggtgttgt acttgtttag cgagcgattt atgacctatg gcaaggcgta tggcttatgc
     3721 gaatggttcg aggtagagca gtttcagaac ccctgaaatt tcagggagat gtcatggata
     3781 gggtggacag tagcagccac tgcattaggg gataatcaga tgtccttcac tcgacgcaaa
     3841 tttgttctgg ggatgggaac agtaatcttt tttactggtt ctgcgtcttc tctgttagcg
     3901 aacacgatgc aagaaaagaa agttcggtat gccatgattc atgacgagtc acggtgcaat
     3961 ggctgtaata tttgcgcccg cgcatgccgt aaaactaacc acgtccccgc tcagggaagc
     4021 cgtttatcga tagcacacat tccagtcacc gataacgaca acgaaacgca gtatcacttt
     4081 tttcgccagt catgccagca ctgtgaagat gctccttgca tcgacgtttg cccgacaggg
     4141 gcgtcgtggc gtgatgagca ggggatcgtg cgggtagaaa aatcgcagtg catcggctgc
     4201 agttattgca ttggcgcttg tccttaccag gttcgctacc ttaaccctgt cactaaagtg
     4261 gcagataaat gtgatttctg cgctgagtcc cgactggcaa aaggctttcc gcccatttgc
     4321 gtcagcgcct gtccggaaca cgcattgatc tttggtcgcg aagatagccc agaaattcag
     4381 gcgtggttgc aggaaaataa atactatcaa tatcagctac ctggtgccgg gaaaccgcac
     4441 ctgtatcgcc ggttcggtca acatttgatt aaaaaggaaa atgtatgaac ccgtcgcaac
     4501 atgctgaaca gtttcagagc cagttggcga actatgtgcc actgttcact ccccaatttt
     4561 ggccggtgtg gttgatcatt gccggactat tgctggtagg gatgtggttg gtgctggggc
     4621 tgcatgcctt gcttcgtgct cgtggcgtga agaaatcagc caacgaccat ggtgagaagg
     4681 tttatcttta cagcaaagcg gtcagattat ggcactggtc gaatgcgtta ctctttgtat
     4741 tgttgctggc aagtgggctg ataaatcact ttgcgctggt gggcgcaact acggttaaaa
     4801 gtctggttgc ggtgcatgaa gtttgcggat ttttgttact ggcatgctgg ctcggctttg
     4861 ttctgatcaa tgccgttggg ggtaatggtc accactatcg cattcgtcgt caggggtggc
     4921 tggagcgagc ggcaaaacaa acgcgattct atttgtttgg cattatgcag ggggaagaac
     4981 atcctttccc ggcaacaacc cagtctaaat ttaatccctt acagcaggtc gcctatgttg
     5041 gtgtcatgta tggattgctg ccattgttac tattgacggg gctgctgtgt ctctatccgc
     5101 aagccgtggg agatatgttt cctggcgtaa gatactggtt attgcaggcg cattttgctc
     5161 tggcatttat aagcctcttt tttatcttcg gtcatcttta tctttgcacc acggggcgta
     5221 cgccacacga aacctttaaa agcatggtcg atggctatca ccggcactaa cgaatgatca
     5281 ttacacgagc agacctgcga gaatggcgta tcggagcggt gatgtatcgc tggtttctgc
     5341 gccatttccc acgcggcggt agttatgccg atattcatca tgcactgatt gaggaaggat
     5401 atacggactg ggcggagagt ctggttgagt atgcgtggaa aaaatggctg gcggatgaaa
     5461 acttcgcgca tcaggaagtt tcttctatgc aaaaactggc cacagatccc ggagagagag
     5521 cattttgcag tcagtttgcc cgcagcgacg atcatgctcg catcggctgt tgtgaggata
     5581 atgccagaat cgcaaccgct gggtatgccg cgcaaattgc cagtatgggt tatagcgtgc
     5641 ggattggaag tgtcggcttt aacagtcaca ttggcagctc cggtgaacgc gcacgggttg
     5701 ctgtaaccgg taattcttcg cggataagca gcgctggaga tagcagtcgg attgcgaata
     5761 ccggaatgcg agtacgcgtt tgtacattgg gtgaacgatg tcatgtcgcc agtaatggtg
     5821 acctggtgca aatcgccagc tttggcgcaa acgccagaat tgccaacagc ggcgacaatg
     5881 tacacatcat tgccagcggt gaagattcaa caatagtgag cacgggcgtg gtcgactcaa
     5941 ttattctcgg gccaggggga agcgccgcac tggcatatca tgacggcgaa agagttcgct
     6001 tcgccgtcgc cattgaaggt gaaaacaata ttcgagccgg agtccgctat cgccttaatg
     6061 aacagcatca gtttgttgag tgctgatagc tttacatttg attaaacgaa agacgccgac
     6121 gagcacggga tgcaggcttt acgaccgcct gagctatcgc ctcaccaatt tctgcacatg
     6181 ccgtaagtcc ctgtacgaat ggctggtcgt gcatcaacca gggcaacgcg ccgaacgcta
     6241 cgcgcccacg aatttcttcg ggttgctgta gcgtataatc ttcgccaaca tcagggattt
     6301 catcccctgt tttctgtaat tgttcgcgta gtccagggaa aggaatatct ttcactttaa
     6361 gcggacgctg cccacgggca tcaataaaaa cgtcaaacga gtagctattg tcttccgttt
     6421 tcaggacggt gcgcgactca ttaatttcca tttcgtagtc ttcaccgagg gcgagaatat
     6481 gaatgattcc cgcttcacgt aaggccagta gtcgacgaat agactctgaa gggattgccg
     6541 cataattatc gatgaaaacc cgggcaaggc ctttactgaa ccgtttatgg tcgtgttcat
     6601 tcagatgagg aacaatttcc tgtacggctt catgcaggcg cagaatgaca taacgccagg
     6661 gaacagtatg tttttctcgt ttattgcgtt cgacttcctg gagatttttt tctgcccagt
     6721 cgaatgggtc gcgttgtttg cgctcggcaa accaggcttg agcaaaggaa tcgacattca
     6781 ggctttctaa ggctatgcgt tggctccagt ctggatcagc aaatttgatt tcctctacta
     6841 tcaatctaaa tacccgatcc aaaaggccca cttcgccttt ttgaatctca gcatttaatg
     6901 cttgatcggt gacgatgtgt aagggctcgt agggaatagg gcaatagaaa tcggcttcgg
     6961 gtaaaatacc cgtacgcgac atcaacgtaa tatttagctt ttcacttgcg ttatcgcggt
     7021 gaaaaacgac gtgttgttta tcatcttcaa tgaacgaacc atgctgaata gccactgcca
     7081 ttgccgcatc cagtccgctc aaggatgttc ccataatacc cacgttacac gcatcgacct
     7141 ttgcttccat caggcctgac cacgggctgg gaaaatacgt tcgggttgct tcttcttcat
     7201 caggccagac gtgacccgtg gcgatcaccg ctaaatcaaa cgtctcgctg ggtaaatcct
     7261 gatttgtagc gagcatgacg ccagcatttg taatttgcag atcggtaacc tggcatgatt
     7321 cataaacagc cactgcaaat ttttgctgtc gtgcctggtc tactaatcgt aaaaattgat
     7381 cgcggaaata ttcgcccagc agaattcgcg gtaaaaactg acgatcgtgc aaggtttctt
     7441 ttttaacgcc ataacgctgg agatggctgg cttcttgctt ttgtagccat tcgagatacg
     7501 tacaataaat cggcggtatt tcaatactgg caatatttgc cagcatcatt tttgagtttt
     7561 cctcatcact gtatggcatc ccgacaccgg cttcatcagc ctgctcgaag atagaaatag
     7621 aaagtggagt ttgttgctgt agaagcgaga ataaggtgta gatccccgta ggcccggcac
     7681 ccacaatagc aatttttttc attgttactt acctgtacgc attctttcga atttagtgtt
     7741 atcagataag tttggttgag atttttagaa tttgagaaat ttcagacagg gatgaaaagg
     7801 gcgctaaccg cccttgatta aattctgtca ggcgagtttt gcctgattga tttgactaag
     7861 tggctcattg acatcgaaaa ctttggcgac aacttcctca acgccgagat ttttcagtcg
     7921 ggcagtatgt ttttccagat aggcaagggc gcttttttca ttagtaaaaa ggtagatccc
     7981 accggcttcg tggttctttt cactttctgt ccataccttc cacagaaaac caggttcctg
     8041 attaatcgac tcagcaagcg gtttaagctg ctcagccatt gcgtcgccaa atgggccatt
     8101 gaaagaaaaa tgaagttgta acaaagttgc catagcgatt ctccttctga taacgcatac
     8161 gaagacagtg taaatctttt aacgtattgg atatagagaa gaaacagagg gaaaaaggag
     8221 aagaccagat taagaaaatc tggtgcgtcc gagtggactc gaaccaccga cccccaccat
     8281 gtcaaggtgg tgctctaacc aactgagcta cggacgcagg atggtgcgtt caattggact
     8341 cgaaccaacg acccccacca tgtcaaggtg gtgctctaac caactgagct atgaacgcaa
     8401 cgttgtaggt gacaacgggg acgaatatta gcggcagagt gggaaggtgg caagaggcaa
     8461 aacgtaattt tctgcgctat ttcgaccgtt tgcagagctt ttaagcaaat tggctatatt
     8521 ttgttgattt gcaagggcga tttttattca ggatcgcatt tacatcagat acaacccaat
     8581 aatccgtgta tcagagttta aacagagttg tttgccacca tctcaaaatc tgtcgtggat
     8641 aaatcctccg tagcctttac acttaggtag caataaaggc acacagatgc agcaaaggaa
     8701 taaatcatgg gatctgatgc gaaaaacttg atgagcgacg ggaacgtgca aattgttaag
     8761 accggcgagg tcattggcgc gacgcaactt actgaaggtg agttaattgt tgaagctggc
     8821 ggaagagccg aaaataccgt ggtcacgggg gctggctggt tgaaagtggc aaccggtggg
     8881 atcgccaaat gcacacagta cggcaacaat ggcacgctat cggtcagcga cggtgccatt
     8941 gccacagata ttgttcagtc cgagggaggc gcaattagtc tctctacgct cgctacggtt
     9001 aatggccgcc atcccgaagg tgaattcagc gttgataaag gttatgcctg cggtttgttg
     9061 ctggaaaatg gcggtaacct gcgtgtactg gaaggccatc gcgcggaaaa aattattctc
     9121 gatcaagagg gtggcctgtt ggtcaatggg acaacctcag cggtcgtggt agatgaaggt
     9181 ggtgaattgt tggtgtatcc aggtggggaa gccagcaatt gtgagattaa tcagggcggc
     9241 gtttttatgc tggcggggaa agccagtgat acattgcttg ctggtggcac catgaataat
     9301 ctcggtggtg aagactctga cactattgtt gagaatggat ccacttatcg tctggggacg
     9361 gatggtcttc agctctacag ttccggtaag acgcaaaacc tgtccgttaa tgtgggtggt
     9421 cgggctgaag tgcatgccgg tacgctggaa aatgcggtaa tacaaggtgg gacagtcatc
     9481 ctgttgtcac ccaccagcgc ggacgaaaat tttgtcgtag aggaagatcg cgcaccggtt
     9541 gaactgaccg gtagtgttgc attactggac ggcgcttcaa tgattattgg ctatggcgca
     9601 gatctgcaac aatcaacgat tactgtacag cagggcggtg tattgattct cgacggcagt
     9661 acggtaaaag gtgacagtgt cactttcagt gttggtagca tcaatctaaa tggcggaaaa
     9721 ctgtggctaa tcactggtgc ggcaacgcat gtgcaactga aagtgaaacg cctgcgcgga
     9781 gagggagcga tttgcctgca aaccagtgcg aaagaaattt cacctgactt catcaatgtg
     9841 aaaggggaag ttactggtga tatacacgtt gagataacag atgccagtcg gcaaactctg
     9901 tgtaacgcac tgaaactaca gccagacgaa gacgggattg gcgcaacgct ccagcctgcg
     9961 taaaaacaca ggcgcaaccc gtgaaggttg cgccttgtct ttactagcga gctgctcgtt
    10021 gcagaatgat ggctgacggc agacgttgca ggaaccgcat acgcagcatc atcataatgg
    10081 ctgccgacgt caggccaata ataaagccta tccagaagcc tgctggcccc ataggttcaa
    10141 cgaccagatc ggtcagtgcc agaatatagc cgcttggcaa gcccagcacc cagtaagccg
    10201 taaaggtaat atagaaaatg gaacgcgtat ctttatatcc acgcaaaatc ccactgccaa
    10261 tcacctggat tgagtcagaa atctgatata ccgccgccag caacatcaaa tgcgcagcca
    10321 gcgttacaac ctcgggattg tcgttgtaca acagggcgat ttgctcccgc agtgaaaccg
    10381 tgaaaatggc cgtcagggtt gccatacaga cacccaccat aagcccggtc cgcgcagcgg
    10441 tttgcgcatc cagcgttgag ccctgaccca gacgataacc tacgcggatg gttaccgctg
    10501 ccgccagcga catgggaagt acgaacatca gtgaactaaa gttcagggca atctgatgtc
    10561 ctgcgacatc aacaataccg agcggagaca ctaacagagc cacgacggca aacagcgtta
    10621 cttcaaagaa cagcgccagc gcaattggca aaccgagttg aatcagtcgt ttcataaccg
    10681 caggatcggg cttcgcagtg cctttttcgt tacgaatatc gcgcatcgag cgggcgcgtt
    10741 taatgtaaga aaccatggca aggaacatga cccaatacac cgcagcagta gccacgccac
    10801 aaccaacgcc accgagctca ggcataccga aatgaccgta aataaagata tagttcaccg
    10861 gaatgttcac cagcaggccg ataaagccca tcaccatacc cggcttggtt tttgccagac
    10921 cttcacactg gttacgggca acctggaaga aaagatagcc cggcgcgccc catagcaacg
    10981 cacgcagata acccacggct ttgtccgcca gagccggatc gatgttttcc atggagcgga
    11041 taatgtaacc tgcattccac agcaccagca taatgagaac ggaaacaaaa cccgccagcc
    11101 agaaaccttg tcgcacctga tgcgcaatgc gctcacgtcg accggaacca tttaattgcg
    11161 cgataaccgg cgttaatgcc agcagcagtc cgtgaccaaa gaggatcgcc ggaagccaga
    11221 tagaagtacc gatagcgacc gccgccatgt cggtggcact atagccgccc gccatcacgg
    11281 tatcgacaaa acccatcgca gtttgggcga tttgcgcgag aatcaccggg attgctaatg
    11341 ctaataacag acgcgcttca ctgatatact tctgcacgtg aacacctttt atttgtagtt
    11401 atatgagaga ctaaaaagcc gcctgaatgg gcagcaaaaa gaagaagcag gggaaattca
    11461 gtctattgta gcgaggattt attaattctc cagtgaaaaa atagtcattc ttaccccttc
    11521 actggtaagg gcgtttgcca cctgctattg tgctgaacag aatgtcttaa ctgatttcag
    11581 gagttgtaag tatgtttacg gggattgtac agggcaccgc aaaactggtg tcgattgacg
    11641 agaaaccaaa ttttcgcaca catgtggtgg agttacccga ccacatgctg gacggtctgg
    11701 aaaccggtgc ttccgtggcg cataacggtt gctgcctgac cgtgacggaa attaacggca
    11761 accatgtcag ttttgacctg atgaaagaaa cgttacgcat taccaatctt ggcgatttaa
    11821 aagtggggga ttgggtaaac gttgagcgtg cggcgaaatt cagtgatgaa attggcggac
    11881 acttaatgtc aggtcatatt atgaccaccg ctgaagtggc gaaaatatta acctcggaaa
    11941 ataatcgcca gatctggttt aaagtccagg atagtcagtt gatgaaatat attctgtaca
    12001 aaggatttat tggcatcgac ggtattagcc tgaccgtcgg tgaagtcacg ccaacgcgtt
    12061 tttgcgtcca tttaattccg gaaacactgg aacgcacgac tcttgggaag aaaaaacttg
    12121 gcgcacgcgt caacattgaa atcgatccac aaactcaggc agtggtagat acggtagaac
    12181 gagtgctggc ggcacgagaa aatgccatga atcaaccagg tacagaagcc tgatctgaaa
    12241 tcgcccccgg agagggggcg atagaatagc ctttagcgag ccactcgaag gccgttttca
    12301 acaccgcgtg agaacacgac ctgccagagc tgaatatcac gggcgcggaa agcacccgca
    12361 caggcattca gataataggt aaacatccgt ttaaagcgtt cactgtagtt atccgcaatt
    12421 tctggccatg cggcgaggaa tcgttcatac cacgccatca acgtagtatc gtaatcagca
    12481 ccgaagttat gccagtcttc catcacaaag tggggttcgc tggactgagc aatctggcgt
    12541 acagagggca ggcaaccgtt cggaaaaata tatttattaa tccagggatc aacattcaga
    12601 tcggtttttt tcgaaccgat agtatggagc aggaatatgc cttccggttt caaattacga
    12661 tccaccaccg caaaataggt atcgtaattt ttcggtccga cgtgctcgaa catccccaca
    12721 gaaacaatac gatcaaactg gtcgttcagg tcacgataat cttgcagcaa aatggtgaca
    12781 tccaggcctt cacagcgttc ctgagccatt ttttgctgtt cggcagaaat ggtgacgccg
    12841 accacgctta cgtcataatt agacgccatg tagtgcgcca gtccacccca gccacagcca
    12901 atatccagta cgcgcatccc tggttttaac tgcaattttt cacaaatcat tttgagcttc
    12961 gcctgctggg ctgattccag attatcggca tctttccagt aagcacagga atattgcatg
    13021 aagggatcaa gcatgcggct gaacaagtca ttacccaaat cgtaatgctc tttgccgact
    13081 atccaggcac gttttttact ctgcagattg aagagacgag cgccggcaat acgcagcgtg
    13141 tctttgaaat gatgggggag ttggttctcg agacctgcgc gtaagacttt gctaaaaaac
    13201 atatccagtc ggtcacattc ccaccagcca tccatataac tttcgcctaa ccccaacgat
    13261 ccttcctgca gaacgcgttt aaaaaaatcg gggtttttca cacgaatatc cgccggggca
    13321 gaaccgttaa tggctatacc ggcacggcta agtaattcgt tggcgatacg gtaccagttg
    13381 tcatccggta cactgacttc ttctatacac gatgaactca tagtttctcc atcactggtc
    13441 ggtgatcaga accttaaaac agcgtagacg ctttttgggc tttgtgagaa atctcgcaga
    13501 aaaaaccgcg agtctataat ccgacgtaga acagagggaa aggaggaacc cttgccgaaa
    13561 tgaccatcca tagtgagtcg ggaacgatcc tgtccccgta aaaacataat atttatcgta
    13621 gctttaacaa ctcgaaatta gtataggccg ctaatttcaa tcatacaaca aaattttgtt
    13681 agcagcctaa gtataatcat atcgatatag atcagtgtga ttcgctatga gcgacttcgg
    13741 cattgccatg attctgacag tctacttctt cacaacgttg catcatgtaa cccagagcga
    13801 ccagcactac tgttgataac atcacgctgg tggtggtgag caatggcgtg ctgatactga
    13861 tcagccagga aactaccaga cttgcgagga agcacagacc cagttgtaga gtgttctgca
    13921 acgctgcggc gcgaccggtt gcatgtggga agggacgcag cgcctgggcg acaacaatcg
    13981 ggtagatcgc gccattggca atcgccatca cacagaatgg gatcaggatt tcgactagcg
    14041 acacatggct aatgaagccc gcagcccagg tcgcaatgac gctgacagca aacagtacca
    14101 gcaaccacgg taataactgc ttgccttgcc atttctgcag tgcggcgcga cagccataac
    14161 caccaatcag aaacgcgata gtttgcggga cataacttaa accaataact gccgggctgt
    14221 agcccatttc actaaggatg aacggtgagc cggtcagcca tgcaaaaaaa ctggctgaac
    14281 aggccgcata tatcagcacg ttgccgcgat aggttttaga acgtagcagg tcggtaaagg
    14341 tcagaccatc ctgactattg ttacgggcct tcgtcgtggg tttgagccag aaaataggca
    14401 gaatcagcac cacggtaatg gcaaacaggg tggcgaaaat cgcctgccag gaaaaatgga
    14461 ccagcagcca gcttcctaac agaggagcca gtgccggaga tagacccacc agcggcatga
    14521 tggtcgcaaa aatacggtta actttctgtg aaggataata atccgtcact aacgcttgcc
    14581 agataactgc cgcggcgcag acacccacag cctgtacaaa acgcaatacc agcagcgtag
    14641 cggcgttttc tacccacagc atccccagac tacctaacgc aaaaattgtc aggccgatta
    14701 ataataccgg tttacgacca taacggtcgg agagcggccc ccacagaagc tgggctgcgg
    14761 caaaaccggc aaggaacaga ctaaggctgg cactgacagc agacgcaggc gtttgcaggt
    14821 cggcctgtat ggcggcgaaa gcaggcagat acatatcggt tgccagaaaa ccgagtacgc
    14881 tcaaacccgc cagccagact aaaaatcttt tcccaggttg catgtatatt ctctaaatct
    14941 ctcaattaaa caccgccgca aagtgtaggg agtgaaatta gtgttgtgaa acggtaatat
    15001 ttgatgactg gtttcaaaaa ttttgcaggc agactatgtg gtcagaatat tcactcgaag
    15061 ttgttgatgc ggtagcgcgt aatggtagtt ttagcgctgc ggcacaggag ttgcatcgcg
    15121 ttccttctgc ggtcagctat accgtgcgtc agctggaaga gtggctggcg gtgccgctgt
    15181 ttgaacgacg tcaccgtgat gtggaactga ccgctgccgg ggcgtggttt ctcaaagaag
    15241 ggcgctctgt tgtcaaaaaa atgcagatca cccggcagca atgtcagcag atagcgaacg
    15301 gctggcgcgg tcagttagct atcgcagtgg ataatattgt caggccagaa cgtacacggc
    15361 agatgatcgt tgatttttat cgccattttg atgatgtcga acttcttgtc tttcaggaag
    15421 tgttcaacgg tgtctgggat gcgctttccg acgggcgcgt ggaactggcg attggcgcaa
    15481 cacgcgcgat tccggtaggc ggtcgttatg ccttccgcga tatggggatg ctaagctgga
    15541 gttgcgttgt tgccagccac cacccgctgg cgttgatgga tggcccgttc agcgatgata
    15601 cgttgcgcaa ctggccgtcg ttggtgcgcg aagacacctc gcgaacgcta cctaaacgta
    15661 ttacctggtt gctggataac caaaaaagac ttgtcgtgcc tgactgggaa tcatcggcaa
    15721 cctgtatctc ggcaggatta tgcataggga tggtgccaac acatttcgcc aaaccgtggc
    15781 ttaatgaggg gaagtgggta gcactagaac tggaaaaccc cttcccggat tcggcatgtt
    15841 gtctgacatg gcagcaaaat gatatgtcgc ctgcgttaac ctggttgctt gagtatctcg
    15901 gcgatagtga gacgctgaat aaggagtggc tgcgggagcc ggaagagact cccgcaacgg
    15961 gtgattaacg acgatagtcg cggaacgggc cgtcagccac ggagcggcgt tcaatcaagc
    16021 gcggatgcac ttcaatggac tgcggttctt cgcgtttatt gacgatacga tccaacagca
    16081 tgttgaacgc cgtttcgccc agcgaatctt ttggctgatg gatcgtggtc agcgctggcg
    16141 taaaatagcg ggcgttgcgc acgttatcat aaccgatcag cgaaacatcc tgcggcacgc
    16201 gcagacccat ttcatcagca gcacaaagtg cgcccattgc catgatatcg ccaccacaga
    16261 agacggcagt agggcgatgc ggttgcgaca gtatttgctg catggcgcga taaccggatt
    16321 caggttcaaa gtcaccctgt acaatccagc tttccggcac tttgatcatc gcttcttcca
    16381 tcgccttcat aaaaccggca aggcggcctg cgccggtgtt acgttccagc gggccaggga
    16441 taacgccgat ttcgcgatga ccgcgttcaa tcagataacg cccggccatg tagccgcctt
    16501 cgaatgcgtt atcaatgacc gcatcggtga agtcagcttt tgcttcaccc cagtccatca
    16561 ccaccattgg gatatggcga tactcttcca gcatcgccag caacggctct gggtactcag
    16621 aacacatcac cagcagacca tcgacgcgtt tttgcgccat catcgacaga taagcccgct
    16681 gtttctcaag attgttccac gcattgccca gaatcagggt gtaacctttc tggaagcaat
    16741 ttttttcaac cgcttcaatg atctcggcaa aataggccgc ttcgctgctg gtcgccagca
    16801 aaccgataga cttggtgtgg ttaaccttca ggctacgcgc caccgcgcta ggggagtagt
    16861 gtaattcttt aatcgctgcc cacacggcgt tgcgcgtttc ttcagcgacg aaacgtgttt
    16921 tgttgatcac gtgtgacaca gttgtagtgg aaacgtttgc tcgtttcgct acatctttta
    16981 ttgttgccat tccatttcac tccagaccct aacttcagct cctgcaaaat cgcaaggtaa
    17041 acgtttgcct ttacacacct tacgcacaac aaaagtgttg cggtacgccg gaaaaacgac
    17101 acggagcgtc aggaaggggt caatggccag tacgcaaata gtgtaagcgt ggaattttgg
    17161 ccgatcttga cgaaaagggg aagtggtaaa aacacttatc attataaatg acgggagatt
    17221 ttttcatcac agtgtgtaaa aatgcgtaat atcacttttt gtgtggtaat aaaaaaggag
    17281 aaaacttgat gagcaccgac cttaaatttt cactggtaac aacgattatc gtcctcggtt
    17341 tgatcgtggt cgtgggtttg actgccgcgc tgcactgatt tcctctcgag ggagcgcgtt
    17401 ctccctcgca tctccttcct gtttgttagc aaaatggcaa aagtgttttc tctttttgtt
    17461 attttcattt ttttgtgatt gctgtcatgc tttcgccact ttaatgaata tcgcgtaatg
    17521 acgcgaagag gtggagataa ggcatgaaaa ttaactatcc gttgctggcg ctggcgattg
    17581 gcgcgtttgg tatcgggaca acggagttct cgccaatggg cttgttgccc gtcattgcgc
    17641 gcggtgtgga tgtctcgatt cccgctgccg gaatgttaat cagtgcttat gcagttggcg
    17701 taatggttgg cgcgccgctg atgacgcttc tactttctca tcgtgcccgc cgcagtgcgt
    17761 tgattttcct gatggcgatt ttcacgcttg gcaacgtact ttccgccatc gcgccggatt
    17821 atatgaccct gatgctttca cgcattttga ccagcctgaa tcacggggca ttttttggtt
    17881 tgggttcagt cgtggccgca agcgtggtgc caaaacataa acaggccagc gcagttgcca
    17941 ctatgtttat ggggttaacc ctggcaaata tcggtggcgt gccggcggcg acctggttgg
    18001 gtgaaaccat cggctggcgg atgtcatttc tggcaacggc ggggctggga gtgatttcaa
    18061 tggtaagtct gttcttctca ttacctaaag gtggtgcagg ggcacgacct gaagtgaaaa
    18121 aagagctggc ggtattaatg cgtccgcagg tgctgtctgc attgctgacg acggtactgg
    18181 gagctggtgc aatgtttacc ctctacacct atatctctcc ggtactgcaa agtattaccc
    18241 acgcaacacc ggtgttcgtc acagcaatgc tggtgctgat tggtgtcgga ttctctatcg
    18301 gtaactatct cggcggcaaa ctggcagatc gttcagttaa cggcacgttg aaaggctttt
    18361 tgttgctgct gatggtgatt atgctggcaa tcccgttcct ggcccgcaat gagttcggcg
    18421 cagctattag catggtggtg tggggcgctg caacctttgc ggtcgtaccg ccgttacaga
    18481 tgcgcgtgat gcgtgtcgcc agtgaagcgc caggtctgtc ttcatcagtc aatattggtg
    18541 cctttaatct tggaaatgcg ctgggagcag ctgctggtgg tgcggtaatt tccgctgggc
    18601 tgggatacag ctttgtgccg gtgatggggg cgattgtcgc gggactggca ttattgctgg
    18661 tgtttatgtc agccagaaaa caacctgaaa cagtttgcgt tgccaacagc taattaaaca
    18721 aaaacgcaga aggataacct tctgcgttac agttgctggc aacgtgcgat ttatcttgcc
    18781 gaatgcgctg ttaacgcctc atccgtgttg gcatggcgcg cgaagcgcat caggtaatgt
    18841 tgcatttgcc atcagttatt atgcagcgag atttttcgct acgaattccc agttcaccag
    18901 cgcccagaag tgctccagat agccaggacg tgcattgcga tagtcgatgt aataagcgtg
    18961 ttcccagaca tcaacggtca gcagcggagt tgcatcggtg gtcagtggag tacccgcgtt
    19021 agaggttgaa acgatagcca gtttgccatc gctgtttttc accagccagg tccagccaga
    19081 accaaagttt ttgatcgctg catcagtaaa ctgagctttg aaatcggcaa agctgccaaa
    19141 agatgcggcg atagcttcag cgacttttcc agtcggttcg ccacccgcgt tcggtgccag
    19201 gcagttccag tagaaagtat ggttccagac ctgagctgcg ttgttgaata cgccaccttc
    19261 agagctgcga ataatctctt ccagtgattt accttcaaac gcggtacctt taatcaggtt
    19321 gttcaggtta gtgacataag tctgatgatg cttgccatag tgatactcga tggtttccgc
    19381 agaaatgtgc ggtgccagag catctttagc atatggtagt gcaggtaatt cgaatgacat
    19441 tgctactctc ctttattatt aatttgcata cgtacaatag ccttattgtg cgtatggata
    19501 gggtagcaaa ttaaaaggtg agacaaaaga tgaacttacc ctgttgccgt aacaacaggg
    19561 taaagtttta gcgaagtgtt tttggggtca ttacccgacg agcgccaaca tagtggcgct
    19621 gccagtagtc ttcactgaga gaagtgattt gaatttcttg gcccgtgcgc ggtgactgaa
    19681 taaatttgcc gttgccgaca tacacgccga catgatcggc tgtaccgcgt ccctgagtac
    19741 ggaagaagac caggtcgccg tttttcagtt cactacgttc gataggtgct gcatcacgca
    19801 ggtgatacat ttcattcgcc gtacgcggaa tacgaatttt caccaaatct ttataagcgt
    19861 aataaaccag gccgctgcaa tcaaaaccgg tacgcggtga gctgccgccc caacgatatg
    19921 gcttaccaat ttgttgcatc agtttattca ttgccacttt cgtggctttt tgcaccttcg
    19981 ctttgtgcgc gtcggcaagg gttcctgacg cggcattttt gactttcaca caatgcgatt
    20041 tatagccctt acgtttggta cacttttcgg taaagttggc tgaggccgtt ttattgacgg
    20101 catgtttgct gcgagttttg gcgtttttag aagcggtaat ggaagatttt ttaacggaag
    20161 aagcggtttt gcttttggtc gtacttttac tggcggcttt tttcgctgtt ttctggctgg
    20221 ctttctgggt ctttttggtg gttgccgtgc ttttcttttt atctgctttt ttggtgatat
    20281 gagtagtagc agaactctcc ctggcttgct ttgaagcatg ggccataggc gttaaaggca
    20341 gggtggtaaa aagtaaagca cagagcgtga tcgaaatacg gtttatccgc gccactgagc
    20401 gtccctctgt taaggcaggt ctatcatgcc tgcgtctgat ttacaaaact tgatgattct
    20461 aatgcataga agaagagaaa gttaatagct tttattgtta aaaaatacgt ttcgttggcg
    20521 agcgcaaaaa aatgcagcag tgaaagctaa aaatggttat aaatacgcca gtttgccgtg
    20581 cgtcagggaa gaaacgaata tttgaaatgc aacaaataga gcaaagggat aaaatagcaa
    20641 aagcgtgatg aaaatgttat tttccgatgc cgcactgacc cgctacaatg tcagattact
    20701 gccgtaaaga agtttgagga agcaagacaa tgagcaccac tatcgaaaaa atccaacgcc
    20761 agattgctga aaacccgatc ctgctgtaca tgaaaggttc accgaaactg ccgagctgcg
    20821 gtttctctgc ccaggcagtc caggcgcttg ccgcatgtgg cgaacgtttt gcctatgttg
    20881 atatcctgca gaatccggac attcgtgcgg aactgccgaa atatgctaac tggccgacct
    20941 tcccgcaact gtgggttgac ggcgagctgg tcggcggttg tgatatcgtg atcgaaatgt
    21001 atcagcgtgg cgaactgcag cagctgatca aagaaactgc cgctaaatac aagtctgaag
    21061 agccggacgc tgaataatcc gtcctgcaat actgacggat gcgaacgggc attcgtcagt
    21121 cctttctcta tccccaatcc agcccttgtg gcgaacttga aaatcccgcc tcacgcagca
    21181 gcgtaaacat cggcgtttgc cggaccggta ggtcattcac ttctgttagc gtaaagcgca
    21241 gccgtggttc gcgacgcagt gcggtagtca gcgcgtggaa aatctctggg gcgagtaatt
    21301 cctctttttc ctgccagacc agcatttttt tgccaccttg cgccagatag agtaacaatt
    21361 tgccgccaga aacgaccacc agcgcacccg cccgacgcgt tggtatcagc gttgccggat
    21421 gtgcaggcca gggcagaaga tttccccatg cattagccgg atcgttggca gagagcgcca
    21481 ctggtgtata gtggcgcgtt tgcgtggctt gtgtcgccag atcgcgcaat cggtcaatag
    21541 tcagacgttc agcgaattgc gcgccaccca gaccttctac aaaacgacct cgcataattc
    21601 gcccggagtc ttccatactt cgacaaagcg tttgcatcga tggaaacccg ccagggatat
    21661 tttcggctat caccgcctga cgactgatga tgccgtagcg gtcgagcata ttttccgcca
    21721 gcgccagcat cctttcggta tcgtttagtg gctccacctg caataacgac cagcgtccag
    21781 caagctttgg tgtgttgtaa gataccagcg gcgagacggg ttgcgcatag acaggacgtc
    21841 cacgacgagc ccggtgactg cggcgagttg aggtgcgtgt gttggtactg ctgcgggtga
    21901 gggcgcgtaa cggtgcccaa atgtcgctgg tgatgacgcc ttgccagacc agcgcccata
    21961 acgcctcttg catggctgag ggatcaaccg attcgccgat tttatcgcgt atccgttggc
    22021 tgatttgttg tgcaaaccag gctcctccgt cagccagaac cgtgactatc gcttgctgca
    22081 gcgccgaact attcgcctga ttcgcttcgg cgggagtgaa cgattctgcg gcatattcct
    22141 gtagatgcag tgccaccaga ccgtcatctt cacccagctt tttttgcccc gaccagataa
    22201 ccgcaccggt tgccagtaat tcatcgagca tttctggcga atagtcgcgt acgcgggcag
    22261 gcaggatctg gctttcccag agtgaggcgg gtaaaccgac tccggcaagc tgttcgatca
    22321 cccgcatgac gccatccacg ccctcataaa cgcctggcga tgttgaggca aagagtgccg
    22381 ggctaccatc ggtggcgggt aatacgccct gacgttccag cagcaatcgc gcataggtcg
    22441 tggccgcaac gggacgcgtc gcttctctgg cggcttgcag cgagcgcaaa cgcagacgac
    22501 gaaatacttc atcgctcacc cagatgtctt gttgcagatt catcaccagc ccctgttcgc
    22561 gcagttgctg aagttgctct tcgacaatgg caataccgag actaaactca tgagccagtt
    22621 gttcagacgt gaccagagca tgagcccgga gatagcgcag aaagaggtcg cgaagcgggt
    22681 aactgactcg gtgcaaataa atctctggca gtgactctgg tagtcgtacg ccgagagcat
    22741 cgcgcagcct ggcggcatca tccatacagg caagacgctc ctgcccgcta atcatggtcg
    22801 ggaagattcg ttttactgca agaagatttt ccagatacga cgcaatctct tcactgctgc
    22861 ctgtatgtcg ttgcgccagg tcttcaacgg tcattggccc cagttcgcgc agcaggtcga
    22921 acaatccttc ttcacctttc gctcttctgc caggagccag tcgttgcaac tcttcttcca
    22981 cctggcgaat gacctgtggg tcgagtaatt cccccggatc gacctgtccg agtagattgc
    23041 gcagtaactc gctgtccagc gacagtacgg atgcccggcg ctctgccagc ggggcgtcgc
    23101 tctggtacat aaattccgcg acatagccga acaataaact ggcggcgaaa ggcgaaggcg
    23161 tagtggtcgt tacatcggat atttgaattt cgccaccgtt caggcgacgc atcaaacgtt
    23221 ccagcgcggg aagatcataa acatcttgca gacattcgcg tagggtttcg agaatgaccg
    23281 gaaaatctgg atatccctga gcgatttcca gcaactgact ggcgcgcagc cgttgttgcc
    23341 agagcggggt gcgatggccc ggcgtgcggc ccggcattaa taatgtccgc gcggcgcatt
    23401 cgcgaaaacg ggcggcgaaa agtgccgagc tgcctaccgc ctcgcggaca atttgcagca
    23461 acttttctgg ttcaaacaaa aaaatcgcgg catcaggcaa tttaccatcg gtgtcaggaa
    23521 tgcgcgcaac aatgccgtca tcactggcaa ccacagacgc gtcagcgccc catagtgcat
    23581 gtattcgccc ggcaatcgcc agcgcccagg gttcatgcac ccgtcttcca tagggagagt
    23641 gcaaaataat acgccagtca ccaatttcat cacggcaccg ttcgaggacc agatgacgac
    23701 tccccggaac gatgcccgtc gcgttgcgct gctcgtcaat caacccctga atattggcga
    23761 tcgtattttc ttctgccaac cacgggggaa tagtcccgga aaagaacgcg ccatccgcca
    23821 gtaaatggag aaaatcgccg atcatctcgc ctaattctgc cggacgtccg ttaccttcac
    23881 cacgccagaa gggaaggcgg gcagaacgac ctggagcagg agtcacaatc acctgatcgc
    23941 gggtgatttg ctggatccgc catgaggtag cgccgagagt gataatgtcg ttcacccgcg
    24001 actcatatac catctcctca tccagttcac ccacccgccg cgaaccggcc tgttcttcac
    24061 cttcgggtaa taacacgcta tacatgccac gatccggaat ggtgccaccg ctggtaaccg
    24121 ccaacagctg agcgccaggt cgggcggtca atatcccggt ctccctgttc cagaccagtt
    24181 ttggtcgaaa agcagaaaaa tcgccagagg gatagcgccc ggaaagcata tccagcgtga
    24241 cgtcaaaaac acgtcttggc agatctttcc acggtgcggc acggcgtacg cgggagtacc
    24301 attcgtctgc ctgtaatgca tccatcgccg cggcggcaac ggtttgctgc gcaaggacgt
    24361 cgagtggatt atgcggcggt gtcaggtttt ccagcctgcc tgcgaacata cactctacaa
    24421 tgactgcgga atcgactaag tcacgcctgg ttcgggggaa aaaaagccct ttagatacac
    24481 cgccaacctg atgtccggcg cgaccaatgc gttgtaaccc actggcaaca gaaagcggcg
    24541 ttgccacctg aatcaccaga tccaccgcgc ccatatcaat ccccagttcg agactggagg
    24601 ttgcgaccac acagcgtaat tcacccgatt tcaacgcctg ttcggtgatt gctcgttgtt
    24661 ctttagagac ggagccgtgg tgtgagcggg caataaaaat gtcgctactt tgtacacggt
    24721 tagaggttgc gccggaggtc gactcgaaat gggccgcatc aacggcgata gatggggaac
    24781 gctgtaagcg tgcggcgtaa agctcattta atcgtgccgt cagtttttcc gccagcccgc
    24841 gtgaattagt aaagacaatg gtcgagcgat ggcgcaaaac ttcatcaagg atacccgttt
    24901 caatatatgg ccagatggag ccttcccggc cggcatggct gtcttcgccg gtgccgctgg
    24961 cgaccgatga gacatcatcc atattggcga ccggtacgac aatccgtatc tgcggatggc
    25021 gcattgcggg cgggttgact accgtaaccg ggcgatcgcc accaagaaat gctgccacat
    25081 cgctggctga acgcacagtg gcagaaaggc caattcgctg tgctgaggtg tggagcagcg
    25141 tatcgagccg ctccatactt aacgccagat gcgcaccacg tttactgccc gctaccgcgt
    25201 ggacttcatc aataattacc gtttcgacgc cgcgtagcgt ttcgcgcgcg cgggaggtca
    25261 gcatcagata gagtgattcg ggtgtggtga tcagaatatc cggcggatta cgggtgagtt
    25321 tgctgcgttc ctgtgcaggc gtatcgccag tacggatccc cacgcgaaga ttgacttccg
    25381 tttcgccgcg ccgccgccgt tcatcggcaa taccctgtaa cgggatctgc aagttgcgct
    25441 gaacgtcggt gcccagagct ttgatcggtg aaatatagag gatgcgtgag gttttacgct
    25501 tatgcgcctc gcgggtatct tcgccgcctt cgcggaagag ctgatcgagg gcgtagagaa
    25561 atgctgccag cgttttcccg gagccagtcg gtgcaatcac cagcgcatgt tcgcctcgcg
    25621 ccgccacatg ccaggtttgc gactggacag cggtcggctg tttaaaggcg cgaagaaacc
    25681 agtcgcgggt cgccggtgaa aacacgtccg gcaggggcga tgaagggttt agattatctg
    25741 ccattaacgc tattccccgt gaatatcatg catgatgcgt ggaatagcct cactatagat
    25801 gcctgaatca ttgcatgtca tcaggcatcg actcgattac acctcttcgg cggcaggtag
    25861 cggccagcct cccagacgtt tccagcggtt gacgatttca caaaacagca cagcagtgcg
    25921 ttcggtgtcg tacagcgcgg agtgcgcctg ggtgctgtcg aagtccatgc cagcggtctg
    25981 gcaagccttt gacaatacgg tttgtccgag tgccagcccg gccagtgcag cagtgtcaaa
    26041 agtggcgaaa gggtggaacg ggttacgttt cagtgaggcg cgttctgcgg cggccatcat
    26101 aaagctgtga tcgaaattgg cgttgtgcgc caccataatg gcgcggttac aaccgctcgc
    26161 tttaatacct ttacgcacaa ctttaaaaat ttcgtgcagc gcctcgtatt cgctgaccgc
    26221 gccgcgatcg ggatcgttcg ggtcaatgcc gttgaaggcg agggcctctg gctgcaaatt
    26281 tgcgccgaca aatggttcga cgtggaaatg taatgtggtg tccggcatca gccagccttg
    26341 ttcatccatt ttcagggtga tggcggcaat ctcaagcagc gcatcggttt tggcgttaaa
    26401 tccagctgtt tcaacatcga tcacaacagg ataaaaacca cgaaaacggt cgcacagacc
    26461 ggtaagttga gcgttatcgg acatctgatt ctcttaatac ggataaattg cagcgcgcat
    26521 tatgacaaat attacttgtc gatgcagtaa agatgcgggc gcgatgagtt cacgcccggc
    26581 aggagattag ttgcccagac cgcgaccggc gtctttctct tcgattaact caattttgta
    26641 accgtccgga tcttccacaa acgcgataac cgtagtgccg ccttttaccg gacccgcttc
    26701 acgggtgacg ttaccgccgt tctgacggat tttttcgcac gcttcagcgg cgttatctac
    26761 gctaagcgcg atgtgaccat aagcagtgcc gagttcgtat ttatccacgc cccagttgta
    26821 ggtcagttca atcaccgctt cttcggtttc cgggccgtag ccaacaaacg ccagtgagta
    26881 tttgtattcc gggttttcgc tggtacgcag cagtttcatg cccagtactt tggtataaaa
    26941 atcgatggag cgttgcagat cgccaacgcg cagcatggta tgaagaagac gcatctttat
    27001 atcctcaaaa tggctaacag attcaaaatg ttgttttagt atagcggcgg ctttgcgccg
    27061 ctcgcaatgt tggattacag cgtcgggtaa tcagtgtagc cttccgcgcc gccaccgtaa
    27121 aaactttcgg cacgctgtgg gttaagctca gctttgcgtt gcaagcgggc gaccagatcc
    27181 gggttcgcaa tccagtcacg accaaatgcc accgcatcaa ttaacccttt gccgatcagc
    27241 gtttcggctt tttctaccgt gtatgcacct gcgccgataa tcggaccgtg gaaacgggcg
    27301 cgtacttttt cgcggaacgc atcagtatac ggttcacccc ccgcccaatc tggttctgac
    27361 atatgcagat aagcaatgcc gcgtttaccc agttgttcta tcagatacag tgcatcggct
    27421 tcttcattcg ggccgttatc tgtgttctgg aaagtaccga ttggcgaaac gcgaatgcca
    27481 atgcgatcgg caccccattc ttcaatcccg gcatcgacca cttccagcac cagacgtgcg
    27541 cgattttcca cgctgccgcc gtactgatcg gtacgatggt ttgaagaagg agaaaggaac
    27601 tgatgcagca aataaccatg agcagagtgg agctctacca gatcaaaacc ggcttcacgc
    27661 gcgttagcaa tggcctgacg gaaatcattg acgatacccg gaatctcttc cagttcaagc
    27721 gcacgcggca tggatgtttc aacacggatc gcctgaccat tttcatcgcg cagagaagta
    27781 cgtgttcccg cgctaagtgc ggaaggcgct accggtgcct gaccgccagg ttgcaggctg
    27841 gcgtgagaaa tgcgtccggt gtgccacagc tgtacggcca tatgaccatt ttcagcatga
    27901 acgccagcgg taattttttt ccatgcggca atttgttccg gactatggat gccaggtgca
    27961 ccagcatacc cttttgcctg ggcagaaatt tgcgtggctt cgctaataat caaaccggca
    28021 ctggcacgtt ggcgatagta ttccgccatc aacggggtag ggatgtcacc cggttcaata
    28081 ctgcgcagac gcgtcagcgg tgccataaaa atacggtttg ccgccgtgat cgcgcccact
    28141 ttcagtgggg aatacagttt ttcagatgac ataaaggctc ccggtcgtct ggtgataaaa
    28201 aatgcctgct aaacggcagg cgtcgcaata atggttttta catgtgccag cgcgttttcc
    28261 agtggctcga aactgcgcga aattttggcc tgcagattcg cacccagcca tagtgcgtaa
    28321 agcacttgtg cctgttgcag cggttcgcca caaaaggtta aacaatggct atcacggcca
    28381 ttttccagcg cctgcgagag cagggcgatc acgccgcgag cgcctttatc catcgcgcta
    28441 cgcatatctt ctgacagatc gcacacttcg gcagagagtt ttactgtcag gcaaccactg
    28501 atggttccat gttggcaaaa ctggttcagt gtttgctggt aataagccag tatgcggtcg
    28561 cggtagttac cttcgccgga ttgcagcaac tcagtcagtc gttggtgata tgtggcgtaa
    28621 tggcgctcaa gcatggcaac gccaaacgct tctttagagc gaaagtagtg atagaaggac
    28681 cctttcggca cttcagcggt ttttagtaat tcgcttagcc ccatcccggt gaatccacgt
    28741 tgcaggcaaa gttgctcgcc cgtcgccagg agatgttcgc gagtatcatg ttcggtgtgt
    28801 ttgttcatgc gttggagtgt agtagaccag tcggtctagt gcaagcaaag ttgctacttt
    28861 gggcattaac agcttcaata atggtaataa tcttatttga ggagggcgaa gtggcggagc
    28921 aattagagtt ctttcccgtt cagagcccgt gccggggaat ttgccagtct gacgaacgcg
    28981 ggttttgtcg cggctgtttt cgtagtcggg atgagcgttt taactggaat aaaatgagcg
    29041 atggcgaaaa acaagaggtg ctgcgccttt gtcggcagcg attaatgcgc aaattacgcg
    29101 caaataagcc agcgtcatca gacgaaccgg agcaaccatc actcttttga gcctaatctt
    29161 gcgtatactc ctctcatatc tgataagagg aagcgattat ggttcagcgt attactattg
    29221 cgccgcaagg cccggagttt tcccgttttg tgatgggcta ctggcgattg atggactgga
    29281 atatgtccgc ccgccagctg gtcagtttta ttgaagagca tctggatctc ggcgtgacca
    29341 ccgtggacca tgctgatatt tatggtggct atcagtgcga agcggcgttt ggcgaggcgc
    29401 tgaaactggc tcctcacctt cgtgaacgga tggaaatcgt cagtaaatgc ggtatcgcga
    29461 cgaccgcgcg tgaagaaaac gtcattggtc attacattac tgaccgcgat cacatcatta
    29521 agagcgccga acagtcgcta attaatcttg cgaccgatta tctggatctg ctgttaatcc
    29581 accgaccaga cccgttaatg gatgccgatg aagtggcgga cgcgtttaaa catctgcatc
    29641 agagcggcaa agtgcgccat tttggcgtat cgaactttac gcctgcgcaa tttgccctgt
    29701 tgcaatcacg tctgccgttt acccttgcca ctaatcaggt ggaaatatcc ccggtgcatc
    29761 agccgttact gctggatggc acgctcgacc aactgcaaca actgcgtgtt cgtccgatgg
    29821 cgtggtcctg ccttggtggt ggtcgtctgt ttaatgatga ttatttccag ccgctgcgtg
    29881 atgaactggc tgtggtggcg gaggagttaa acgcgggctc gattgaacag gtggtttacg
    29941 cctgggtatt acgtttaccg tcgcagccgc tgccaattat cggctcaggt aaaattgagc
    30001 gtgtacgggc agctgtcgaa gctgaaacac tgaaaatgac ccgtcaacaa tggttccgta
    30061 tccgtaaagc ggcattgggt tacgacgtac cgtaagcact tttaggaata gccgccgttc
    30121 aaaaatgtgt cactggttta cacttattca ggaatgcaca atgaacggag gtcctatgaa
    30181 acgttttagt ctggccattc tggcgctggt tgttgcgacc ggcgcacaag ctgccagtga
    30241 aaaagtcgag atgaacctcg tcacgtcgca aggggttggg cagtcaattg gtagcgtcac
    30301 cattactgaa accgataaag gtctggagtt ttcgcccgat ctgaaagcat taccccccgg
    30361 tgaacatggc ttccatattc atgccaaagg aagctgccag ccagccacca aagatggcaa
    30421 agccagcgcc gcggaatccg caggcgggca tcttgatcca caaaataccg gtaaacatga
    30481 agggccagaa ggtgccgggc atttaggcga tctgcctgca ctggtcgtca ataatgacgg
    30541 caaagctacc gatgccgtca tcgcacctcg tctgaaatca ctggatgaag tcaaagacaa
    30601 agcactgatg gtccacgttg gcggcgataa tatgtccgat caacctaaac cgctgggcgg
    30661 tggcggtgaa cgctatgcct gtggtgtaat taagtaatta agaggccagc gtaccttgtg
    30721 gcggtgcttg ctcaagttgc gaaagcgagc agtacagccg ccagacaatt gccgccagct
    30781 cgcgggcggc aggttgatga tggcgggcaa ggctgtcgca aatccgctgt aattcttcaa
    30841 gtgtggccgc cagtgatttt tgctgaacgc cacgctcact catcactccc cgcaacagtg
    30901 aaatacagtt atcccgtact cgcgataacg gatcggaacg cgattcccag tcgcgcaatt
    30961 gccagacaac atgagaacag ttcagcagca ctacacccca gcgtaataac caacggcgag
    31021 ccagcgcatc ctggctgtta ctcaactgac tgacgtgatg ataagtgagc gattcaaatt
    31081 cgctttcact cagtgttgga tggcggctta gttgatcgac aaaatcccgg cgcagcgcgc
    31141 gtatatggcg gcggcttttg cgagcatccg atcctggacg cagaatggcg aacgctaacc
    31201 acgccaacgc gacgccaacg atttttgcca gattatcgtt aagaaaatca gcaaaatcat
    31261 acaccggggg attagtgaca gcgataaacg aacccataaa aacaatcagt tgcccccaca
    31321 gtgcggcaaa tttgggcatc tgcaatttaa gaagctgcat tgtcgccagc agtggaaaga
    31381 gaaacagtaa aaattgccac agatcgctaa tctgcaccat caggccaaat ttgaccacaa
    31441 agctgaatag cgaaagtaac accagcgtgc gcatcagcag tgacaacgac ttaaacggtg
    31501 ctgcgacggc ggagtagagt acgcagctaa ttgctgccag cgttaatgca ttggaaccgg
    31561 catcccattg cgaagcaata ctccatgcgc caatcatcat caacgtacaa aatgtacgca
    31621 gtccgctcca catagcttcg gcgttatcgg tatgacgagc caggccggat gtccgtggga
    31681 gtctggtatg atcatccaca ccgctttgca gatgttgtaa ttcctgacta ctttgcagat
    31741 aaaggcggca aaaataacgt agtcgctgcc agaaggcgac gtgccgatag tcggcgacgt
    31801 tggtcgggcg tagcggggcg ataatacgtg cgacggtgta aacatctgtt tgcgaactgg
    31861 cgagcgccgt cagcagctgt tcgagaattt ctcgtgtggc acatggcggt gagggccagt
    31921 tgagcaacat acgtcgcagg ctggagatga cactggtcat acgtaattgc tggtggagca
    31981 gcgcattaag gcgtgcgttt tgctggcgaa aacgatagtg gctccagaaa gcctggatac
    32041 ggagcaaatt catggtcagt atctgtccaa tcaccccctc atgtgctgca cgaatggcat
    32101 cggttgtttc aggctgccag agtaaactgg catgttccag taatcgggcg tgcatgtttt
    32161 tcaatgcggt taaaagggct gtagcatcgg aactgctcgg caggatcatc atcatcatgc
    32221 cgccgcacaa aataccgaca atcacctcgc aaacgcgcgc ctgagcgata tcccacagct
    32281 ggctggcctc agtaatatta accatcggaa aggcgatgat ggcagctgtg tagcccgcca
    32341 gttgaaatgc atacgcgaca ttattcgtga agtgcgcaca ggcccaggta caaaagccaa
    32401 gccacgccgc catgctcaaa agaaaaaacc acggctcatt gagcgtatgt ccggcaagaa
    32461 gcagtgccgc aatggctccg agcaaactgc cagcgatgcg tccgaggctt ttgctgatga
    32521 caccgccaac ggtgggaaag ctaaccacgg cagccgaggt catcgcccaa tagggttcat
    32581 ccagatttaa ataataggca accgtcagcg ccagacacat ggcaatggta ttgcgtaacg
    32641 cataacgcca ttgcgccagc gtggccctga accagggcaa attgcgcaag gaccatgacg
    32701 atgcgttcat tattgaccta cggcaatact acaggtggtg cccgacacca gcgtgacatc
    32761 gcctgggact ttatccagcg caaagcggac gggaactcgt tgggcgaggc gaacccaggg
    32821 gacgttaggt ttcacatccg gaataaggtt tgagtcactt tcaacgctct gatcataaat
    32881 cgcccgaccg atactggaaa cgtgaccctg taacgttttg ttatcactat agagggtaat
    32941 ttgtgctggt gcaccttcgc ggatatggcg caacttggtt tcttcaaaat aaccaatgac
    33001 gtaaaacgag tggctgtcga caagggcaaa cagcggtttc ccggtgtcag cgtaatcgcc
    33061 gatgcgggta gtgaggttag tcacccatcc actcaccggg gcgcgaattt ccgtttgcgc
    33121 cagttgccat tgtgcctgtt tcagcgtcgc ctgtgcggca tcaacactgg cctgcatcgc
    33181 tttaacattg aggttagcgg tatcgagttc ttcggcagaa ataaaatttt gtgagagatg
    33241 acgacgacga ttcgcttcgt tattggcttt tgcgaggtca gattgcgctt tcgccaactg
    33301 agcctgtgcg ttcagctcgg cgatctgaaa gggcgtttta tcgatcgtga gcagaagatc
    33361 ccccgcgtta accagttggt tgtcttttat attcagctca acaatacgtc ccgacacctg
    33421 aggggtgata gaaacctgtt ctgcgcgtat tttcccgtcg cgtgtccacg gagattgcat
    33481 gtaataattc cacagccacc atccggcaag aaccgcaact accgctacaa tgattgttga
    33541 gaaatactta attgttttaa ttgacatagt taccacgcaa tcagtattgc cagggaaagg
    33601 caaacgcaaa tcgcaaacag cgataaatcc attaacaagg gatgccagat gtcaccggcg
    33661 tagatccagc cacgaagcag gcggtgtacg acaagccaga tgacgaatcc aaacgcgaag
    33721 gctttgaaaa acggaggaaa gtagacggac gcaccgaaca ccagatcttg taagggcaat
    33781 cctgttgcat tgagcataaa cttcacaaag tagattcctt tacgaccgaa tatattgcgt
    33841 tagattaata aatattcttt aagtgcgaaa aatttacgcg caatttctga aaaatatgtc
    33901 tgacggtaac caaatgcagc aatacatttg ttttagcaat acaattgctg cacactattc
    33961 taaaagccgc ataatatctt agcaagctaa ttataaggag atgaaattgg aatcgccact
    34021 aggttctgat ctggcacggt tggtgcgcat atggcgtgct ctgatagacc atcgcctgaa
    34081 accgctggag ttaacacaaa cccattgggt tacgttacac aatatccatc agttacctcc
    34141 agaccagtcg caaattcaac tggcaaaagc gattggcatc gagcagccat cactggtccg
    34201 tactctggac caactggaag aaaaagggtt aatttcgcgt caaacttgtg ccagcgatcg
    34261 tcgggctaaa cgtattaaac tgacggaaaa ggcagagccg ctgatcagcg aaatggaagc
    34321 tgttattaac aaaacccgcg cggaaatttt acatggcatc tccgcagagg aactggaaca
    34381 actgattaag ctcatcgcaa aacttgagca taatatcatt gagttacagg ccaaagggtg
    34441 aaatgaaggg ggcgtgtggt cagtaacctg accacacgca aacttattta gcgcggggaa
    34501 acggtcacct gactgccatt gctggccagt acgacacgtt ggcccggaga gaaacgagtg
    34561 ttgccttgtt tctgtaccac catgatggta ttaccatcgt ctttacgaat ttccagctcg
    34621 acaccctgcg ttttgttcat tgcactctgt acgccctgac cggctacgcc acctgcaaca
    34681 gcgcctgcag cagtagccag agaacgcccg gttccgccac caacagtatt ccccaggaaa
    34741 ccaccaagaa cagcaccgcc aattgcgccg ataacgttgg aatcatcacc gccctgaatc
    34801 tgtaccggac gtacgttaac gatggtgcca tagctgacat tctgtacttg tttcgcttca
    34861 gaagcggtat aaacatcccc tgacagggtg tcgttattaa cacaaccgac aagagacaga
    34921 cctaccattg aaacaaccaa tacgcgttta atcattgaaa aatctcctgt tcaccatgaa
    34981 acgccacgca agcatccctc atggtcaaag tatatggcat atgtgaatcc atggttacat
    35041 gttctaccaa atatgagcaa atcataatga aagtaggatc aaccaattat aaacaaaaca
    35101 ttcatgatga ttattgattt gaagataatg ttttactacc aacggtaaaa atcggtcatt
    35161 ccgcatggta ttcacgaaaa gtttatgttt gagtggtgcg caaacgtcag attgaaaaag
    35221 gatgaactat gaaatcgggc cgctttattg gcgttatgtc aggcaccagc cttgatggtg
    35281 ttgatgttgt gttggcgaca attgatgaac accgggtcgc acagctggca agtttgagct
    35341 ggccgatccc ggtgtctctg aaacaggctg tactggatat ttgccagggc cagcagctta
    35401 tactttctca gtttggacag cttgatactc aactcgggcg actttttgct gatgcggtca
    35461 atgccttgct taaggaacaa aacctgcagg cgagagatat cgttgcgatc ggctgtcacg
    35521 gtcaaaccgt ctggcatgaa ccgacgggcg tggcaccaca cactttacag attggcgata
    35581 acaatcaaat tgtggcacgc accggaatta cggttgtcgg tgattttcgc cgtcgcgata
    35641 ttgccttggg aggacaaggc gcaccgctgg tacctgcgtt ccatcatgcg ctgctggctc
    35701 acccaaccga gcgacgaatg gtgctcaata ttggcggcat cgccaatctg tcactgctca
    35761 ttcctgggca gccggttggg ggctacgata ccggtcctgg taacatgctg atggatgcct
    35821 ggatctggcg tcaggccggt aaaccttacg ataaagatgc cgagtgggca cgggcgggta
    35881 aagttattct cccactgctg caaaatatgc tcagcgaccc gtatttctcg caacccgcac
    35941 cgaaaagcac cggacgcgaa tactttaact acggctggct ggagcgccat ttgcgccatt
    36001 ttccgggtgt tgatccccgt gatgtgcagg ccacactggc agaactcacc gccgtgacca
    36061 tttctgaaca agttttgttg agcggtggct gtgaacgatt gatggtatgt ggtggaggaa
    36121 gtcgtaatcc gctactcatg gcgcgtctgg cggcattact gccaggcaca gaagtcacca
    36181 ccactgatgc cgttggcatt agtggcgatg acatggaagc attggctttc gcctggcttg
    36241 cctggcggac gcttgcggga ttaccaggaa atctgccttc cgtcactggc gcaagccagg
    36301 agacggtact cggggctatt ttccccgcta acccgtgata tcagagttaa ctgaattttt
    36361 cttaccgcgt ctgccgttac acttggaacg ttagggaggg cgtattgccc tccagaccag
    36421 gaaagtcttc gggatatgtc tatgaaaaaa ctgttactga tctgtttgcc ggtccttctc
    36481 accggctgta gcgcgttcaa tcaactgttt gagcgcatgc aaaccgatac gctggaatac
    36541 cagtgtgatg aaaaaccgtt gacggtcaaa ctgaataatc cgcgccagga agtcagtttt
    36601 gtttacgata atcaactact gcatctcaaa cagggcattt ctgcgtctgg tgcacgttac
    36661 accgacggga tctatgtttt ctggtcgaaa ggcgaggaag cgactgtcta taaacgcgac
    36721 cgcatcgtct tgaataactg tcagttacaa aatccacagc gttgagattt ttccaggggc
    36781 ggcgcacaat agcgtcaccc actgacaatc cgtaaagaaa accatgtctg ataacgacga
    36841 attgcagcaa atcgcgcatc tgcgccgtga atacaccaaa ggcggattac gccgccgcga
    36901 tcttcccgcc gatccattaa ccctttttga acgctggctc tctcaggcgt gtgaagccaa
    36961 actggcggac cctaccgcga tggtggtcgc taccgtggat gaacatggtc agccttatca
    37021 gcgcatcgtt ttactcaaac attacgacga aaaaggcatg gtgttttaca ccaacctcgg
    37081 cagccgtaaa gcacatcaaa tcgaaaataa tccgcgcgtt agcctgctgt tcccgtggca
    37141 tacccttgag cgccaggtga tggtgatcgg taaagcggaa cgactttcga ctctcgaagt
    37201 gatgaaatat tttcatagtc gcccacgtga tagccagatt ggtgcatggg tttcgaagca
    37261 gtccagtcgc atttctgccc gcggtatcct tgaaagtaaa ttcctggagc tgaagcagaa
    37321 gtttcaacag ggcgaagtgc cattgccgag tttttggggc ggttttcgcg tcagccttga
    37381 acagattgag ttctggcagg gcggtgagca tcgcctgcat gaccgctttt tgtaccagcg
    37441 tgaaaatgat gcgtggaaga ttgatcgtct tgcaccctga aaagatgcaa aaatcttgct
    37501 ttaatcgctg gtactcctga ttctggcact ttattctatg tctatttcgc atctggcgaa
    37561 aagtcgtgta ccggcaaagg tgcagtcgtt atatacatgg agattttgat ggcaagcagt
    37621 aacttgatta aacaattgca agagcggggg ctggtagccc aggtgacgga cgaggaagcg
    37681 ttagcagagc gactggcgca aggcccgatc gcgctctatt gcggcttcga tcctaccgct
    37741 gacagcttgc atttggggca tcttgttcca ttgttatgcc tgaaacgctt ccagcaggcg
    37801 ggccacaagc cggttgcgct ggtaggcggc gcgacgggtc tgattggcga cccgagcttc
    37861 aaagctgctg agcgtaagct gaacaccgaa gaaactgttc aggaatgggt ggacaaaatc
    37921 cgcaagcagg ttgccccgtt cctcgatttc gactgtggag aaaactctgc tatcgcggcg
    37981 aacaactatg actggttcgg caatatgaat gtgctgacct tcctgcgcga tattggcaaa
    38041 cacttctccg ttaaccagat gatcaacaaa gaagcggtta agcagcgtct caaccgtgaa
    38101 gatcagggga tttcgttcac tgagttttcc tacaacctgt tgcagggtta tgacttcgcc
    38161 tgtctgaaca aacagtacgg tgtggtgctg caaattggtg gttctgacca gtggggtaac
    38221 atcacttctg gtatcgacct gacccgtcgt ctgcatcaga atcaggtgtt tggcctgacc
    38281 gttccgctga tcaccaaagc agacggcacc aaatttggta aaactgaagg cggcgcagtc
    38341 tggttggatc cgaagaaaac cagcccgtac aaattctacc agttctggat caacacagcg
    38401 gatgccgacg tttaccgctt cctgaagttc ttcaccttca tgagcattga agagatcaac
    38461 gccctggaag aagaagataa aaacagcggt aaagcaccgc gcgcccagta tgtgcttgca
    38521 gaacaggtga cacgtctggt gcatggtgaa gaaggtttgc aggcggcaaa acgtattacc
    38581 gaatgcctgt tcagcggttc cttgagtgcg ctgagtgaag cggacttcga acagctggcg
    38641 caggacggcg taccgatggt tgagatggag aagggcgcag acctgatgca ggcgctggtc
    38701 gattctgaac tgcaaccttc ccgtggtcag gcgcgtaaaa ctatcgcctc caatgccatc
    38761 accattaacg gtgaaaaaca gtccgatcct gaatacttct ttaaagaaga agatcgtctg
    38821 tttggtcgtt ttaccttatt gcgtcgcggc aaaaagaatt actgtctgat ttgctggaaa
    38881 taatgcatta agtggaaagg gggagtgaga aatcactccc cctggttttt atacagggaa
    38941 catgatgaaa aatattctcg ctatccagtc tcacgttgtt tatggtcatg cgggtaacag
    39001 tgcggcagag tttccgatgc gccgcctggg cgcgaacgtc tggccgctga acaccgttca
    39061 attttctaat cacactcaat acggcaaatg gactggctgc gtgatgccgc ccagccattt
    39121 aaccgaaatt gtgcaaggca ttgccgccat tgataaatta cacacctgtg atgccgtatt
    39181 aagtggctat ctgggttcgg cggagcaggg tgaacatatt ctcggtatcg ttcgtcaggt
    39241 gaaagcagcg aatccgcagg cgaaatattt ttgcgatccg gtaatgggtc atccggaaaa
    39301 aggctgtatc gttgcaccgg gtgtcgcaga gtttcatgtg cggcacggtt tgcctgccag
    39361 cgatatcatt gcgccgaatc tggttgagct ggaaatactc tgtgagcatg cggtaaataa
    39421 cgtcgaagaa gcggttctgg cagcgcgtga actcattgcg caagggccac aaattgtgtt
    39481 ggttaaacac ctggcgcggg ctggctacag ccgtgaccgt tttgaaatgc tgctggttac
    39541 cgccgatgaa gcctggcata tcagccgtcc gctggtggat tttggtatgc gccagccggt
    39601 aggtgttggt gatgtgacga gcggtttact gctggtgaaa ctgcttcagg gggcaacgct
    39661 gcaggaggct ctggaacatg tgaccgctgc agtatacgaa atcatggtga ccaccaaagc
    39721 aatgcaggaa tatgagctgc aagtggttgc tgctcaggat cgtattgcca aaccagaaca
    39781 ttatttcagc gcaacaaagc tctgaaatat ctcatttcgg cccacaacga cctgtgggcc
    39841 gatggctatt actttaagcc ttccgctgac agcgcgtctt gtacttccgg acgttcagcc
    39901 atacgttgca taaatgccgc aatgtgctct aacccttcca gattcagttt caccgcgtat
    39961 gcccagcgca gaaccgtaaa cagataggca tcagcaattg taaatcgttg cccgcagatc
    40021 cagtgctcat ccttcagtgc ctcgttcaca tattgcagct tcttctccag ctgcgcgcga
    40081 actgtcgatt tgtactcttc cggtgtatca gggcgaaaca gaggtgtgaa acctttatgc
    40141 agctcggtgg cgatgtaatt cagccattcg atggttttat agcgggaaat actgtttacc
    40201 ggtgccagca actggcggtc ggggacgctg tcggcaagat actgcataat cgctacgcct
    40261 tccgtcagca aagtaccatc gtccagcagc aatgcaggta cctgtccctt agggttaacg
    40321 gcaaagtaat cgtcaccgtt ttcgagacgt tttttcatta aatccacact gacgagcgta
    40381 aaatcctttc cgctctcacg cagggtgata tgggaagcga gagagcaggc acccggtttg
    40441 tagaacaatt tcatcggtaa ctccttttgt ctgtggttta aagtatggta gtgcgttgct
    40501 gaacaaaaaa aaagccgcta acgaaaagtt agcggcttgt aaaagagttt ccctgaattt
    40561 tacgctacgg ctgctttcgc cgctttgtct gcagcgtcat cctgcgtcat gcggtgcagt
    40621 ttcggcgcgg tcagcagcat cagtactgca ataacggcag tagcgacacc aatctgcaag
    40681 aatacgcgac catagacttc cagtgacatc agtggatcgg taacgttatc cggcacagcc
    40741 atcatacccg caacataacc accaatcagg tttgcaccgg cagtggtcag gaaccagcta
    40801 cccataatga agcccatcag acgctgcgga acgagttgtg caaccattgc cagacccaga
    40861 ccagagatca tcagttctcc gatactctgc aggccatagc ttgcgaccag ccagcttaca
    40921 gacacgatac ctgcgtcaga tgcgaatttt gcacccagcg gcagaatcag gaacgcacca
    40981 gaacacatca ccataccgat tgcaaactta gttggcatcg gcagggtatc gcccatcttg
    41041 ttatagatag cggccagaat cgggctacca atgatgatcc agaacgggtt cagtgcctga
    41101 tactgctcag gttctacggc caggcccaga atggtgtgtt caacgttacg aatcgcaaag
    41161 aagttcagtg acgttggcat ctggctgtac agcacgaaga agataatggc ttcgagcatc
    41221 aggatgaagg caacgatcat tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta
    41281 ccgaagataa ccacgatacc aaaggcaaca acgcccagcg ccatacgcgc aacttcctga
    41341 ttgtgcagca gccaggtggc gatagcgatc agtgccacaa caccaataat ggtcagcagc
    41401 aggttacggt agttgatagg ctcgaagtct ggttttgaac cgtactgttt aacccagcgt
    41461 tggcagaagg cgaagttaac gatagtgatc agcaggccta caacgctcaa cgcaaacgca
    41521 acactccagc cgtatttcgc ggccagccac ggggtggcga tcatagagaa gaaagagccg
    41581 atgttgacgg acatgtagta catggtgaat gcaccgtcca gacgcgggtc gtttttctca
    41641 tagcatgtag aaagcagaga agacgggtta gctttaaaca ggccgttacc gaccgcaata
    41701 gccgccatac ccatataaac gataccggcg tcgtgaccag accaggcaac cagagcataa
    41761 ccaatcgcca gcacaatagc gccaagcata attacgcgtt tagtacccag taccttgtca
    41821 cctaaccagc cgccgatagc gaccagacca taaaccaggg cactaaagga agagaaaagg
    41881 gtgattgaat ccgcttcaga catacccagt tgtttaacca ggtaaacagc cataattcct
    41941 tgtaggccgt aataaccaaa acgttcccat aactcaatcg agaagatgag atagaacgcc
    42001 ttcggttgtt tgaaagcgtt caaactgacg ctttcagttg gtttttggtt tgcagtggac
    42061 acttttacct ctttttttac atcccatatt aacgggggtg ctttctcgcg ttatgcctaa
    42121 aaaacatacg cgcaattgtt atagtgggag gggaaacggc aggtaatgtt cactatcttg
    42181 cactttcaga caagtccttt gtaataatct gttacatata actgaggcgg ggaattcaac
    42241 cattccggga aatgttatcc agcgttaaat ttttcaaaaa acgtaacggc agattttttc
    42301 actaccctga gagggggatg atggcttaac ggcgatatgt tctgctattt agcgtttttc
    42361 ctagtgagat agtccagttt ctgaaaaata gccagtgtaa tgttttgtag gtcaatattt
    42421 gttgcgtgtg taaatttaaa tgattttaac ctagttcttg caatgttttt acattgccac
    42481 agcatcgtga tcttgatcac gaatgaatag aaaatttcgc tattaaaaaa acaattaacc
    42541 tgcatttttg gttatccgca aacggattac ctgatgataa cgctaagcaa tggcatcatt
    42601 gacgttaaaa agatgtttta aaagaggata aagaaaggtt atcaatgggg taatcggtgt
    42661 tacccctttt cttcagatgt caactttctc tttgtattca caaagatctt caataataca
    42721 agagccacag cgaggcttgc gggcaataca ggtataacgc ccgtgcagga tcaaccaatg
    42781 gtggcagtcg actttaaact ctgccggaac cactttcagt agcttttctt ctacctgttc
    42841 gacgtttttc cccggcgcaa attgagtacg attacaaacg cggaaaatgt gcgtgtcgac
    42901 agcaatagta ggccagccga atgcagtgtt taatacgacg ttggctgttt tacgacctac
    42961 accaggcagg gcttcaagcg cagcacgatc ttccggaacc tcgccattat gctgttccag
    43021 taagatacgg caggttttga tgatattttc tgctttgctg ttataaagcc caatggtttt
    43081 gatataggtt ttcacccctt cgacgcccag ttcaagcatc gctgcaggtg tattagccac
    43141 cgggtagagt ttcgccgtcg ccttattaac actgacatcg gtcgcctgag cggaaagcag
    43201 tacggcaatc agcaattcaa aaggcgaact gaaattaagc tcggtggtgg gatgaggatt
    43261 gttctcacgc aggcgagtga ggatctccag gcgttttgct ttgttcatca gacattccct
    43321 gtttcaccgt ttggcaatgc acgttcggca gctgcttcag cacggcgctt tttcattctt
    43381 tcatcaatca ggtatttccc tgccagcatc agtcccaggc caataaatgc acctggtggc
    43441 agcatcgcca gcaggaaagg ggagtcggtg tggaatatct ccacgcgtaa tacttttgcc
    43501 cagctaccta acagcgcatc tgcaccgtca aacaatgtgc cattgccgat aatttcgcgt
    43561 agtgaaccca gcacgaacat ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt
    43621 gccgaaagcg ccggaccttt tttggcggcg aaggcttcag cgcggcccac aacgatacag
    43681 ttagtgacaa tcagcggaat aaaaatccct aatgattgat acaggccaaa ggcgtaggcg
    43741 ttgatcagca tctgtacagc gctgaccacc gaggcgatga tcatcacgta aatgggaatg
    43801 cggatctcgg ctggcgtcca gtgacgcagc gtcgaaatgg tcaagttggt cagcgtcagt
    43861 accagcgtag tcgcaagtcc taaacccaga gcgttagtgg cagtggacgt gaccgccaat
    43921 agcggacaga ggccgagcaa ctggaccagc gcagagttgt ttttccacaa cccctgaaca
    43981 ataacgtctt taatttcgct catgctttat tctccacagg caggaagttg agaaagttgt
    44041 gccggtaacg tctgagcgta caatccggcg cgttttaccg catttaccac cgcgcgggga
    44101 gtaatcgtcg cgccggtgaa ctggtcgaaa tcaccaccat ctttcttcac cgcccagttt
    44161 gcatcatccg caccactgat ttttttaccc gcaaaatggg tgatccagtc agaaaggcgc
    44221 agttcgattt tatcgccaag ccctggcgtt tcgtggtgct ctgtcacgcg cgtgccaagt
    44281 accgtgccgt taaaatcggc tcccaccagc agctgaatcg caccggaata gccatctggc
    44341 gcggttgttt ccagaacggc ggctaccggt ttgtcatcct gtttggcgat gtaaacccga
    44401 tgctcacctt tacctaactc tggcgcagtg accagatagc aactctgtgc cagcgcattg
    44461 ttatagcgtt cggctggcag cacctgatca aataacgcct tttgttgcag actggcctgt
    44521 tcagcaattg tcgttttggt catctggttg atggccgcag ttaaccctgt agaacccgct
    44581 gcaaatagtg ccagtgtaat gccgtgtttt cggatagttt tcagcatggt ttaccctttg
    44641 cgatggccgt agacgcgcgg acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc
    44701 agcaggacgg caaaagccac gccgtcagga tagccgccga aactgcggat caaccagact
    44761 aataaacccg ccagcgcgcc gaacatcaga cgaccacgat tggtcgtaga agcagtaacc
    44821 gggtcagtca aaataaagaa tgcgccgagc atagtcgcac cagacagcag atgaatttgc
    44881 ggtgatgcca gtgtttctgg tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt
    44941 actaagaagc tgaggggaat atgccagcga atcgctttct gccatagcaa ccacacgccg
    45001 ccagccagcc aggcgagatt tacccattgc caaccaacgc ccgccagcat accgctgtag
    45061 atcggatatt gcataatctg ttcaaccgaa tgaccggcac ggacagaggt tttaaatgta
    45121 tccaacggtg tcgcctgact aatgccatca atacctaagc gtagcgtgtt catatcacca
    45181 ccactggtgg tatgtccgct gaaaataacc tgtatggcgt cgataaaacc agggatgttg
    45241 accgcaattt catgtggcgg taaccagctg gtcatctgca ccgggaagga gatcagtaag
    45301 accacataac caatcattgc cggattaaac gggttttgcc ccagaccgcc atacaactgt
    45361 ttagcaatga tcaccgcaaa caccgtaccc agcacgacca tccaccatgg cgcgaggggg
    45421 ggaatactta ccgccagcaa taagcctgtc agcaatgctg agttatcttt caacgttgcg
    45481 gctaccgact gcttgcgtag tttgagtacg agagcttcgg ctaacagagc actgaccgac
    45541 gccaacagga tctgaacgag agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct
    45601 ggcacggctg cgagcaacac caacagcata atgcgcgatg tttggcgctg gttatgggta
    45661 taaggggagc tagctattct gaataccatt tagtcctcgt ttacaacctt ttgctgggcg
    45721 gcttttttgg cctgaacgcg ggcaatagcc gcagcaactg ccgctttgcg cggatcaatc
    45781 tgttcttctg gctccgcatt agcctgttgc tgttccagct tgcgtgcttt ggcacgggca
    45841 atagcagctt cgacggcggc tttgcgcgga tcaacctgtt cttctggttc tgcgttagcc
    45901 tgttgctgtt ccagcttgcg cgctttggca cgggcaatag ccgcttcgac ggcagctttg
    45961 cgcggatcga cctgttcttc tggttccgca ttagcctgtt gctgttccag cttgcatgct
    46021 ttggcacggg caatagcggc ttcgacggcg gctttgcgcg gatcgacctg ttgttctggc
    46081 tcggcattag cctgttgctg ctccagcttg cgcgctttgg cacgggcaat agcggcttcg
    46141 acggcggctt tgcgcggatc gacctgttct tctggttccg cattagcctg ttgctgttcc
    46201 agcttgcgcg ctttggcgcg ggcgatagcg gcttcaacgg cagttttacg tggatcagca
    46261 acggttgctg cgtcgttagt ttgctgcagt tctgcctgtt tcgctctggc ttgcgcttta
    46321 cgggcttccc gtgctgcaat aattgcactg ttatccgggc gttcgcccgc tttaatcaca
    46381 ataggctgtg tagcctgggc ctgtttctct ttcacccgcg ccagagcggc agcaatcgca
    46441 tctttatctt tggctgcagg ttgaacggct gcgctcttat gtcgttcaag gcgagccgct
    46501 ttttcgcgct ccagacgagc ctggcgcgct tcgaaacgcg ctttggcttc tgcggcgcgc
    46561 ttttcttcct gacgaatagc tgcaatttca gctttttcct gacggaaata ttgcaccagg
    46621 ggaatattgc tcgggcaaac ccacgcgcaa gccccacatt caatgcagtc ggcaatgtta
    46681 tgcgtggtag ctttatcgtg ttgctgacct ttgctgaacc agtacaactg ttgcggcaaa
    46741 agatccgcag ggcaggcgtc agcacaggcg ctacaccgga tgcagctttg ttcttcctgt
    46801 ggttcgccaa gctcattggc agagggagcc aacagacagt tggtaatctt tacgaccggg
    46861 acatccagcc atggcaaggt aaagcccatt agcgggccgc ccataatcac catttgatcg
    46921 gcagaggggc agaatccggc gtcattcaat aaatgacgca ctggcgtccc cagccgtgcc
    46981 cagacgttgc ccgggcgagc gattgcttcg ccagtcaggg ttacgacacg ctcggtaatc
    47041 ggctcgccat caataacggc acgtttcact gcataagcag tgccgacgtt ttgcattaat
    47101 acgccgatat cggatgaacg cccgccatgt ggaacctgct tcccggtcag aatgtaggtt
    47161 aattgtttag caccgccaga aggatatttg gttggaatca cccgcagaga aatatcgtga
    47221 gagtccgcca gcaccgcgcg cagcatggaa atcgcctgcg gtttgttatc ttcaatgccg
    47281 ataagaattt cgcgtggctg cagaatatgc gcaagaatgc gaataccctc tacgacctga
    47341 gccgcgcaat cctgcatcaa acggtcatcg gcggtaatgt acggctcgca ctcagcagcg
    47401 ttgataatca acgtttcaat cttatctccg ccaccctgca atttaacgcc tgtcgggaat
    47461 cctgcaccgc ccagcccggc aacaccaaac tgatgaatgc gctcgattaa ctcttcgcga
    47521 ctgcgagagc gataatcggc ccagccgtct cgcgggatcc agcagtcttc accatcggca
    47581 tcaataatca cgcttaattc agctaacgct gaaggatgag ccgtagagtg gggcgcaata
    47641 gccgtaacgg tacccgaggt gggcgcgtga acaggcagca ttttgccgcg accacgggta
    47701 agcggctggc cgcgcaatac tttatcgccg acgctaacgc acaactcacc ttcagcgcca
    47761 atatgctgtt tcagtggaat aacaaaacgc tgcgccagtg gtacctggcg caggggtgta
    47821 ccgttggact gggttttcat ctccggtgga tggatgccgc cgttgaaatc ccagatttta
    47881 ttttttctga atgcagagaa taacttaagc atggtgttcc acgggaatga tacgcacggg
    47941 aatggtgttc agatcccatt tccaggagtc aggtgtttct gcgaccggtt gcaacgagat
    48001 gcagtgcgtc gggcacggat caacacataa attgcagccc gtacagagat cactcattac
    48061 cgtatgcatg gcacgggtag cgccaacgat ggcgtctacc ggacaagcct gaatacattt
    48121 agtgcagcca atacagttat tttcatcaat aaccgccacc atccgcgcag gcgttagctc
    48181 ttgcgcttcg ccatccagcg gctgcggctc gacattaagc aactcggcaa tttttagcat
    48241 cacagcttcg ccaccagggg cgcaacggtt gattttttca ccgttatagc tgatggcttc
    48301 cgcgtagggg cgacagccgg gataaccgca ctgaccacac tggctctgcg gtaagatttc
    48361 gtcaattttc tcaacgaccg gatcgtcttc caccgcaaaa cggcgggagg cataacccag
    48421 aatggcgcca aacgccaggc ccagcaggct cacggcggca acggcaatcc agatagcatt
    48481 cattacaact tcaccaaacc actaaagccc ataaaggcca gagacataag acctgcggta
    48541 attaatgcaa tggcattacc gcgaaaaggt gccgggacat cagccacagc aaggcgttcg
    48601 cggatggcgg cgaagagcac catcaccagc gagaaaccga cagcggcgga aaaaccgtac
    48661 agcgccgact gaaagaaatt gtgcccgaga ttgatattca gcaacgccac gccgagcact
    48721 gcacagttgg tggtgataag cggcaaaaaa atccccagca gacggtaaag caccgggctg
    48781 gttttgcgca ccaccatctc ggtgaactgc acaaccacag caatcaccag aataaacgcc
    48841 agggtgcgca ggtaaatcag attaagcggg atcaaaatcc acgtatcgat aagccaggcg
    48901 caaatagacg ccagcgtcat cacaaacgtt gtagccagcc ccatgcccat cgcggtttcc
    48961 agctttttgg aaacccccat aaacggacag agaccgagaa acttgaccag tacaaagtta
    49021 ttgaccagta cagttccgac aaacagtaac aggtagtcag tcatttttcg gcctgaaata
    49081 aaaaagccgc ctattatccg ttaatccaga gcaggcgaca acaggttaac tgtaaggtta
    49141 ttacggattc acaaacgtcg ctttcacccg cgacgagcgt ttaaaatagg ggacgatcaa
    49201 tgcggttgcc aacaaagtaa acagtaactg gcgaacggct atgccatctt ccacgggtga
    49261 aaaagcgaac gcttttactg ccagcaatac ggaaataagc agccagataa tatagtgttt
    49321 aggaacacaa cgccgacgtt tgaagaaagc gatggtcaac cacagtgtgt aataccacat
    49381 ggcgatagcg gtaataaaag aaacgcccca taagatttgt gtcgtcagtg cttgttcgcc
    49441 gagcgtttga aatgtttgcg gagaagataa cgcagcggta tacagcaaca acgccagcgt
    49501 cgtacttaac agagcgacta aaagccaggc caatgggcca agcaaccagc ctccaatacg
    49561 ttgtggcgtt gtggtggtca taggttctcc ataatagcgg cgcaaacagg caaatctaat
    49621 tttaggggcg ttattataag gcttttataa ccgatcgcta ctttctttat tggacatagt
    49681 gccagacgga cttcggtacc tggccgaggt caaacaaacg cccgccagaa accagctctg
    49741 cgcgacggtg atcggcagca cgatacatat tgatcagttc ctgatcgtcc gtcagggtgt
    49801 aattaagatg gtcgtagagt ttttccaaac tttcgagtga actgattttg cgaaatttta
    49861 ataagtagtc ctgaacagtc ataaaaggtc cataaaataa ttaataccaa aggggtaggt
    49921 attattagtg atgataaaat ttcaccaaaa gtgaacgctt gtgttattac aataataaga
    49981 atgcgctgat ggcaaatatt cagggatgaa aaactgctct gaaaagagta acgcgcttta
    50041 ctatctctga caatcaccaa caacatcgaa caagataata aattcctggt ttaatatccg
    50101 acaagtgaaa acatgcaccc ggacgggcag catgtcgctc tacaagcgca gagcttactt
    50161 gtgttgtacc gtagtactct gttcaggtgg ctgatagttg tcaatgtgac tcgccacgcc
    50221 aagaagaatg actgaaacga caagaacgat ccaacctgtt aattcaataa gacgattcat
    50281 tacagcccac atctctgttg attgatccat taacttcagg gggtaaatgt tacttagcaa
    50341 taatagctcc agcaagattt ttactgaggt ttttgcgata tttagctttt gtcgttggaa
    50401 aattcgctat ttttttgact taagttaaac aacatccctt attgctggca ggttattaaa
    50461 ctgttgagcg agggtaaagg atagtgtcaa atagccatca tacttcaatg agaggcaatg
    50521 acatgagcga caacatccgt gttgggttga ttgggtatgg ttatgcgagc aaaaccttcc
    50581 atgcgcccct gattgcgggc acgcccgggc tggaactggc ggtaatctcc agcagcgatg
    50641 aaacaaaagt aaaagccgac tggccaacgg ttacggttgt ctctgagccg aagcatctgt
    50701 ttaacgatcc caacatagac ctgattgtca ttcctacacc caacgatacc catttcccgt
    50761 tagccaaagc ggcgcttgag gcgggtaaac atgtggtcgt tgataaaccc tttaccgtga
    50821 cactgtcaca agcgcgagag ctggaagcgc tggcaaaaag cctagggcgt gtgctgtctg
    50881 tattccataa ccgtcgctgg gatagcgatt tcctgacgct aaaaggtttg ctcgcggaag
    50941 gcgtgctggg tgaagttgct tactttgagt ctcattttga ccgcttccgt ccgcaggttc
    51001 gcgatcgttg gcgtgaacag ggcggcccag gcagcggtat ctggtacgat ttagcaccgc
    51061 accttcttga tcaggccatt acgctatttg gtttaccggt cagcatgacg gtagatttgg
    51121 cacagttacg gcccggagcg cagtcgaccg attatttcca cgccatcttg tcctatccgc
    51181 agcggcgagt cattttacac ggtaccatgc tggcagctgc tgagtcagca cgttatatcg
    51241 tgcatggatc ccgaggcagt tatgtgaaat atggcctcga tccacaggaa gaacgactga
    51301 aaaatggcga gcgtctgccg caggaaggct ggggctacga tatgcgtgat ggcgtactta
    51361 cccgcgtgga aggtgaggaa cgtatcgaag aaacgctgtt gacagtacca gggaattatc
    51421 cggcttacta tgcggctatt cgtgatgcgt taaatggcga tggtgaaaat ccggttccgg
    51481 caagtcaggc aatccaggta atggagttga ttgagctggg catcgaatcc gccaaacatc
    51541 gcgcgacgct gtgccttgcg tgaaaagagg atggtgcaat tagcaccatc catttttgtt
    51601 acttcgcggc gactttttct cgcagtgcgc gtttttcctc tgcgctgagg aaagccattt
    51661 ccagaccatt aatctgcgcc tggcggattt gctcgcggga taacccagca gccggcgcgg
    51721 caacggtata ttcgtgaatg atatccactc cctgtacgcc gggatcgtca gtgttaatgc
    51781 tggcacgaat gccatgctca aggaacgttt tcagcggatg tgccgccagc tctgccacgg
    51841 tgctggtctg aatattggag gtcagacagg attcaatacc aatttgctgc tcggcgagaa
    51901 aatccatcag cgcccgatct tcaatggctt ttacgccatg tccaatacgt tccgccccca
    51961 gttcacgaat cgcctgccag atgctttccg gcccggcagc ttcgcctgca tggacggtaa
    52021 tatgccagcc cgcatcacgc gcgcggttga agtgagaaag gaacagactt cccgggaaac
    52081 caagttcatc accggctaaa tcaagtgcgg taatctggtc acggtgggct aaaaaggcct
    52141 ccagctcttg ctgacaggcg gcttcgccga aggtccggct cataatgccg ataagcttcg
    52201 cctgcacacc aaaggtgcgg caaccttcac gtacgccatc gatcaccgct tcgacaacac
    52261 ccgctacagg cagctgatgt gccattgcca tgtagcccgg tgaaaaacgc agctcgacat
    52321 agtgcaggcc attacgggct gcatcttcaa tgttttcaaa tgccacgcgg cggcaagcat
    52381 caagagaggc gagaacttta acgccccagt caagtttggt cagaaagctc accagatcgg
    52441 gttcgttggc aatgacctga acgtggggaa tcagtgtttc cagggattgt gcaggaagcg
    52501 agatattata ctggcggcca agttcaagaa tggtctgggg acgaatgttg ccatcaaggt
    52561 ggcgatggat atcagttaat ggcagggtgg tatcaatcat ggtcgcactc tttttctgga
    52621 tataaaagtg cgcaccatta taaaaacaaa gcagggtaaa aatccacgat tgcgcaacga
    52681 ttttttacgt tgcgcaattg gggttaacga acagcgcgga tggcgttaat taatccagca
    52741 acaccttttt ccagtttcga acgcgggcag ccggcattga gacggacaaa accacgacct
    52801 tcttcaccgt aggtataccc cggcatgatc gcgaccttct cttgctcgat aagcgctttt
    52861 tgcaacgcgt tgtcgtcaat attcaatgga cgtagatcaa gccaggccag ataagtggat
    52921 tgcgggatct gccagttgag ttcagggaac gcggcgttca ttttatctgc gatatacgtc
    52981 aggttatctt tcagatagac gcgtaaggca tccagccacg gtgcgccttg ctgataggcg
    53041 gcgatatgag cagttaacgc cagtaccgaa ggggaagaaa gcccatcacg tcctttcagt
    53101 gccgataaat aggcatcacg gctgctgcta ttttctataa tcccgtaagc accggtcagg
    53161 gcgggaatat tgaaactttt cgatcccgac gttagcaatg cccagtctcc gcgcgccaca
    53221 ttactccagg gaatatgcgg ctgctcgccc caaaccatat ccatatggat ttcatcggaa
    53281 ataacccgca caccatgacg ctcgcacagg tcagccatga tctccagttc atcgcacgtc
    53341 cacactttcc cggtaggatt ctgtgggcta cacaggagca taattttaca ttctggtttc
    53401 gccaacacgg cttccagctt gcccatatcg caaaaccagc cgtcagcccg cttctctaaa
    53461 gcgacgggca ttactgtgcg ctggttacct tcaatggcct tgtaaaatgc gtcataggcg
    53521 ggtgtgtgga tcaccacgcc ttcacctttt tcagaccact gacgaatcag ttccgagacc
    53581 atgtagataa cagaagggcc atacaccacc gactgcgtat caattgcggt gtaatgctgg
    53641 gtggaaaacc agtgggcaat agccgcgaga aactcatcgt ttttccagcg gctgtagcca
    53701 aacacgccgt gcatcaggcg ctgattcagc gcctcgataa tgcagggggc agaggcaaaa
    53761 tccatgtctg aaatcgtgaa cggtaacagg tcagcagtgc cgaaacggtc agcgacataa
    53821 tcccactgcg tacaccatgt gccatgacga tccacgacct ttgaaaaatc gaacatatct
    53881 tatccttatg cctggacagt atgcatcaga ccggccattt catcttttac tgactgaact
    53941 tgtggcccga taacaacctg caggttatgt tgattaagtt gtactacgcc aattgcccga
    54001 ttgtccttca gtgcctgcac attaacaagc gacatatctt tcacagacaa acgcagacgg
    54061 gtaatgcagt tatcgaggct gacaatattg tcggcaccgc ctaaggcttc gaggattgcc
    54121 ggaacgttgt aacctgattt acccggcgca ccggcaacgg ctttttcgat tgagctggca
    54181 acttcgctat cgcgccccgg ggttttcaga ttgaagcggg tgatagcgaa acggaagatg
    54241 acgtagtaaa cgacaaacca gattgccgcc accactggca ccatgtacca cttggttgac
    54301 agaccatgca aaataccgaa caccacgaag tcgatgatat tgccgtcggt attaccgatg
    54361 gtgacgccga gcacagacat gacggtgaag ccgaggccgg ttaacagcgc gtggatgaca
    54421 tacagaactg gcgctacgaa caggaacagg aattccagcg gttcggtcgt gccgccaacg
    54481 acacaggcga tcaggccaga aataagcaga cctttaattt tatggcgatt ttccgggcgc
    54541 gcgcagtgat acatagctaa cgctgcacct ggcaggccgc cgagaaacgc aggcatttta
    54601 ccttgcgaaa ggaaacgcgt ggcgctttca gaaaaaccgt gagtggtcgg gcaactcaat
    54661 tgcgcctgga agatggtcag tgcgccgctg acggtttgac cgcagacttc ctgcgtgccg
    54721 cctgcgtcgg taaagcgaat taatgccacc agaatgtgat gcagaccaaa cggcaacagc
    54781 agacgttcac cggtaccaaa cagcatcggt ccgaaatcac ccgcgctgtt gatcatatgg
    54841 cctaagccgc taatacccat ggcgaaaatt ggccagacta atggaatcac cagaccgaca
    54901 aggcccatca ccagcgagga gatgattggc acgaagcgcg taccgccgaa gaatgccagc
    54961 gcatccggca ggcggatatt atggaaacgc tcatggagca tccagacgat aataccggcg
    55021 atcaccgcac cgaggatccc tgtatcgatc gactggatcc caagaatgct ctgaatgtta
    55081 ttggctttca gaaccgcggc gtccgtggtt ggcagaatgc ctttattggt caaccagaag
    55141 tttaccgcga gattcattac cgcataaccg acaaagccag caaatgccgc tacacctttg
    55201 ttttcgcgtg ccaggcccag cgggatggcg atacagaaca tgactggcag gaaactaaaa
    55261 gcaaacgagc caatcttact catccaggta aagatagcct gcaacacggg gttgcccagg
    55321 accgggatca gggtgatgac atcatggctg ctaagagaac taccaatgcc gagcataatg
    55381 ccgcagaacg acaataatgc cacaggtagc atgaaggttt tgcctaactg ctggaagaac
    55441 tcccacagcg tgactttcgg tgctgttttc gccgtcataa aacgactcct cgtagagaac
    55501 aatctggatt cagtaaattg cgcgagaaga taaaacgttt tatcaaattt tagtgaggca
    55561 taaatcacat tacgcaacga taatagcggg tataagataa ataaaaggta aaacgtttta
    55621 tctgtcacat aatcagggag taggtcatct gcatggctac cgccaaaaaa ataaccattc
    55681 atgatgttgc gctggctgcg ggcgtgtcgg taagcaccgt ttcgctggtg ctaagtggca
    55741 aagggcgaat ctctaccgcc acaggagaac gcgttaacgc cgccattgaa gagctgggat
    55801 ttgtgcgcaa tcgtcaggcg tcggcgctgc gcggcgggca aagcggcgtc attggtttga
    55861 tcgtccgtga tttatctgcg ccgttttacg ccgaattaac ggccggattg acggaagctc
    55921 tggaagcgca gggacggatg gtttttttgc ttcacggcgg taaagacggc gagcagctgg
    55981 cacagcggtt ttcactgtta ctgaatcagg gggtcgatgg tgtggtcatt gccggggctg
    56041 caggaagcag cgatgacctg cgacggatgg cagaagaaaa agctatcccg gtgattttcg
    56101 cttcccgtgc cagttatctt gatgatgttg atacggttcg cccggataac atgcaggctg
    56161 cacagttgtt gacggagcat ctcattcgca atgggcatca gcgtatcgcc tggctgggag
    56221 ggcaaagttc ctcattaacc cgggcagaac gggtgggggg ctattgtgca actctactaa
    56281 aatttggcct gccgtttcac agcgattggg tgttggagtg ctcttccagc cagaagcaag
    56341 ccgcggaagc tatcacggcg cttttacgtc ataacccgac catcagtgct gtggtttgct
    56401 ataacgaaac tattgcgatg ggggcatggt ttggtttgct gaaagcaggg cggcaaagcg
    56461 gggaaagcgg agtcgatcgt tactttgaac aacaggtttc gttggcggca tttaccgatg
    56521 caacgccaac cacacttgat gatatacctg ttacctgggc cagcacgcct gcgcgggaac
    56581 ttggtaccac acttgcggat cgcatgatgc aaaaaatcac ccatgaagag acgcattcac
    56641 gcaatcttat tattcccgcc cggctcattg cggcgaaata atcctctctt tatctgctat
    56701 acctggtagt gtcctttcct caaggttaat gataaaaaat gtggcacaca ggcggtgcct
    56761 tttattttta ctgaagttca caggaggttt atgtgtttaa ttctgacaac ctgagactcg
    56821 acggaaaatg tgccatcatc acaggtgccg gtgcaggtat tggtaaagaa atcgccatta
    56881 cattcgcgac agctggcgca tctgtggtgg tcagtgatat taacgccgac gcagctaacc
    56941 atgttgttga tgaaattcaa caactgggtg gtcaggcatt tgcctgccgt tgtgatatta
    57001 cttccgaaca ggaactctct gcactggcag actttgccgt cagtaagctg ggtaaagttg
    57061 atatcctggt taacaacgcc ggtggcggtg gtcctaaacc gtttgatatg ccaatggcag
    57121 attttcgccg tgcttatgaa ctgaatgtgt tttctttttt ccatctgtca caacttgttg
    57181 cgccagaaat ggaaaaaaat ggcggtggcg ttattctgac catcacttct atggcggcag
    57241 aaaataaaaa tataaacatg acttcttatg catcatctaa agctgcggcc agtcatctgg
    57301 tcagaaatat ggcgtttgac ctgggtgaaa aaaatattcg ggtgaatggc attgcgccgg
    57361 gggcaatatt aaccgatgcc ctgaaatccg ttattacacc agaaattgaa caaaaaatgt
    57421 tacagcacac gccgatcaga cgtctgggcc aaccgcaaga tattgctaac gcggcgctgt
    57481 tcctttgctc gcctgcagcc agctgggtaa gcggacaaat tctcaccgtc tccggtggtg
    57541 gggtacagga gctcaattaa tacactaacg gaccggtaaa caaccgtgcg tgttgtttac
    57601 cgggataaac tcatcaacgt ctcagctaaa taactggcag ccaaattacg gctattggtt
    57661 aaccaatttc cgagtgaaaa gtatacgaat agagtgtgcc ttcgcactat tcaacagcaa
    57721 tgataggcgc tcacctgaca acgcggtaaa ctagttattc acgctaacta taatggttta
    57781 atgatggata acatgcagac tgaagcacaa ccgacacgga cccggatcct caatgctgcc
    57841 agagagattt tttcagaaaa tggatttcac agtgcctcga tgaaagccat ctgtaaatct
    57901 tgcgccatta gtcccgggac gctctatcac catttcatct ccaaagaagc cttgattcag
    57961 gcgattatct tacaggacca ggagagggcg ctggcccgtt tccgggaacc gattgaaggg
    58021 attcatttcg ttgactatat ggtcgagtct attgtctctc tcacccatga ggcctttgga
    58081 caacgggcgc tggtggtcga aattatggcg gaagggatgc gtaacccaca ggtcgccgcc
    58141 atgcttaaaa ataagcatat gacgatcacg gaatttgttg cccagcggat gcgtgatgcc
    58201 cagcaaaaag gcgagataag cccagacatc aacacggcaa tgacttcacg tttactgctg
    58261 gatctgacct acggtgtact ggccgatatc gaagcggaag atctgtcgcg tgaagcgtcg
    58321 tttgctcagg gattgcgggc gatgattggc ggtatcttaa ccgcatcctg atcctctcat
    58381 ttcgcgggct ggtgataact gtgcccgcgt ttcatatcgt aatttctctg tgcaaaaatt
    58441 atccttcccg ggttcggaga attcccccca aaatattcac tgtagccata tgtcatgaga
    58501 gtttatcgtt cccattatgc tcgaacgaac gttcggttgc ttattttatg gcttctgtca
    58561 acgccgtttt aaagattaat gcgatctata tcacgctgtg ggtattgcag tttttggttt
    58621 tttgatcccg gtgtcagttc tttttatttc catttctctt ccatgggttt ctcacagata
    58681 actgtgtgca acacagaatt ggttaactaa tcagattaaa ggttgaccag tatttttatc
    58741 ttaatgagga gtcccttatg ttacgtcctg tagaaacccc aacccgtgaa atcaaaaaac
    58801 tcgacggtct gtgggcattc agtctggatc gcgaaaactg tggaattgat cagcgttggt
    58861 gggaaagcgt gttacaagaa atccgggcaa ttgctgtgcc gggaagtttt aacgatcagt
    58921 tcgccgatgc agatattcgt aattatgtgg gcaacgtctg gtatcagcgc gaagtcttta
    58981 taccgaaagg ttgggcgggc cagcgtatcg tactgcgttt cgatgcggtc actcattacg
    59041 gcaaagtgtg ggtaaataat caggaagtga tggagcatca gggtggctat acgccgtttg
    59101 aagccgatgt cacgccgtat gttattgccg ggaaaagtgt acgtatcacc gtttgtgtga
    59161 acaatgaact gaactggcag actatcccgc cgggaatggt gattaccgat gaaaacggca
    59221 agaaaaagca gtcttacttc catgatttct ttaactatgc cgggatccat cgcagcgtaa
    59281 tgctctacac cacgccgaac acctgggtgg acgatatcac cgtggtgacg catgtcgcgc
    59341 aagactgtaa ccacgcgtct gttgactggc aggtggtagc aaatggtgat gtcagcgttg
    59401 aactgcgtga tgcggatcag caggtggttg caactggaca aggcaccagc gggactttgc
    59461 aagtggtgaa tccgcacctc tggcaaccgg gtgaaggtta tctctatgaa ctgtgcgtca
    59521 cagccaaaag ccagacagag tgtgatatct acccgctgcg cgtcggcatc cggtcagtgg
    59581 cagtgaagga cgaacagttc ctgattaacc acaaaccgtt ctactttact ggctttggtc
    59641 gtcatgaaga tgcggatttg cgcggcaaag gattcgataa cgtgctgatg gtgcacgatc
    59701 acgcactaat ggactggatt ggggccaact cctaccgtac ctcgcattac ccttacgctg
    59761 aagagatgct cgactgggca gatgaacatg gcatcgtggt gattgatgaa actgcagctg
    59821 tcggctttaa cctctcttta ggcattggtt tcgaagcggg caacaagccg aaagaactgt
    59881 acagcgaaga ggcagtcaac ggggaaaccc agcaggcgca cttacaggcg attaaagagc
    59941 tgattgcgcg tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc aacgaaccgg
    60001 atacccgtcc gcaaggtgca cgggaatatt tcgcgccact ggcggaagca acgcgtaaac
    60061 tcgatccgac gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct cacaccgata
    60121 ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta ttacggttgg tatgtccaaa
    60181 gcggcgattt ggaaacggca gagaaggttc tggaaaaaga acttctggcc tggcaggaga
    60241 aactgcatca gccgattatc atcaccgaat acggtgtgga tacgttagcc ggcctgcact
    60301 caatgtacac cgacatgtgg agtgaagagt atcagtgtgc atggctggat atgtatcacc
    60361 gcgtctttga tcgcgtcagc gctgtcgtcg gtgagcaggt atggaatttc gccgattttg
    60421 cgacctcgca aggcatattg cgcgttgggg gtaacaagaa agggatcttc actcgcgacc
    60481 gcaaaccgaa gtctgcggct tttctgctgc aaaaacgctg gaccggcatg aacttcggtg
    60541 aaaaaccgca gcagggaggc aaacaatgaa tcaacaactc tcctggcgcg ccatcgtcgg
    60601 ctacagcctc ggtgacgtcg ccaataactt cgccttcgca atgggggcgc tcttcctgtt
    60661 gagttactac accgacgtcg ctggcgtcgg tgccgctgcg gcgggcacca tgctgttact
    60721 ggtgcgggta ttcgatgcct tcgccgatgt ctttgccgga cgagtggtgg acagtgtgaa
    60781 tacccgatgg ggaaaattcc gcccgttttt actcttcggt actgcgccgt taatgatctt
    60841 cagcgtgctg gtattctggg taccgaccga ctggagccat agcagcaaag tagtgtatgc
    60901 atatttgact tacatgggcc tcgggctttg ctacagcctg gtgaatattc cttatggttc
    60961 acttgctacc gcaatgaccc aacaaccaca atcccgcgcc cgtctgggcg cggctcgcgg
    61021 gattgccgct tcattgactt ttgtctgcct ggcatttctg ataggaccga gcattaagaa
    61081 ctccagcccg gaagagatgg tgtcggtata tcatttctgg acaattgtgc tggcgattgc
    61141 cggaatggtg ctttacttca tctgcttcaa atcgacgcgt gagaatgtgg tacgtatcgt
    61201 ggcgcagccg tcattgaaga tcagtctgca aaccctgaaa cggaatcgcc cgctgtttat
    61261 gttgtgcatc ggtgcgctgt gtgtgctgat ttcgaccttc gcggtcagcg cctcgtcgtt
    61321 gttctacgtg cgctatgtgt taaatgatac cgggctgttc actgtgctgg tactggtgca
    61381 aaacctggtc ggtactgtgg catcggcacc gctggtgccg gggatggtcg cgaggatcgg
    61441 taaaaagaat accttcctga ttggcgcttt gctgggaacc tgcggttatc tgctgttctt
    61501 ctgggtttcc gtctggtcac tgccggtggc gttggttgcg ttggccatcg catcaattgg
    61561 ccagggcgtt accatgaccg tgatgtgggc actggaagct gataccgtag aatacggtga
    61621 atacctgacc ggcgtgcgaa ttgaagggct cacctattca ctattctcat ttacccgtaa
    61681 atgcggtcag gcaatcggag gttcaattcc tgcctttatt ttgggattaa gcggatatat
    61741 cgccaatcag gcgcaaacgc cggaagttat tatgggcatc cgcacatcaa ttgccttagt
    61801 accttgcgga tttatgctac tggcattcgt cattatctgg ttttatccgc tcacggataa
    61861 aaaattcaaa gaaatcgttg ttgaaattga taatcgtaaa aaagttcagc agcaattaat
    61921 caacgatatc actagttaat attcaataaa aataatcaga acatcaaagg tgcaactatg
    61981 agaaaaatag tggccatggc cgttatttgc ctgacggctg cctctggcct tacctctgct
    62041 tacgcggcgc aaccggctga cgatgaagcg ggactacgca tcagactgaa aaacgaatta
    62101 cgcagggctg ataagcccag tgctggcacg ggaagagata tttacgcatg ggtacaggga
    62161 ggattgctcg atttcaatag tggttattat tccaatatta ttggcgttga aggcggggcg
    62221 tattatgttt ataaattagg tgctcgtgct gatatgagta cccggtggta tcttgatggt
    62281 gataaaagtt ttggctttgc cctgggggca gtaaaaataa aacccagtga aaatagcctg
    62341 ctaaaattag gtcgcttcgg gacggattat agttatggta gcttacctta tcgtattccg
    62401 ttaatggctg gcagttcgca acgtacatta ccgacagttt ctgaaggagc attaggttac
    62461 tgggctttaa cgccaaatat tgatctgtgg ggaatgtggc ggtcacgagt atttttatgg
    62521 accgattcaa caaccggtat tcgtgatgaa ggggtgtata acagccagac gggaaaatac
    62581 gataaacatc gcgcacgttc ttttttagcc gccagttggc atgatgatac cagtcgctat
    62641 tctctggggg catcggtaca gaaagatgtt tccaatcaga tacaaagtat tctcgagaaa
    62701 agcataccgc ttgacccgaa ttatacgttg aaaggggagt tgctcggctt ttacgcgcaa
    62761 cttgaaggtt taagtcgtaa taccagccag cccaatgaaa cggcgttggt tagtggacaa
    62821 ttgacctgga atgcgccgtg gggaagcgta tttggcagtg gtggttattt gcgccatgca
    62881 atgaatggtg ccgtggtgga taccgacatt ggctatccct tttcattaag tcttgatcgt
    62941 aaccgtgaag gaatgcagtc ctggcaattg ggtgtcaact atcgtttaac gccgcaattt
    63001 acgctgacat ttgcaccgat tgtgactcgc ggctatgaat ccagtaaacg agatgtgcgg
    63061 attgaaggcg cgggtatctt aggtggtatg aactatcggg tcagcgaagg gccgttacaa
    63121 gggatgaatt tctttcttgc tgccgataaa gggcgggaaa agcgcgatgg cagtgcgctg
    63181 ggcgatcgcc tgaattactg ggatgtgaaa atgagtattc agtatgactt tatgctgaag
    63241 taagaaaaaa tgccggagag aaaaatctcc ggcattttgg ctgtgaatta ctgctgcgga
    63301 atagcgggta cagccggaac gtttaatgcc ggcatcgcaa acatcccaac aaaatcttcc
    63361 agtggcattt tctgcccgtt taacgtgatc tgaccgttag catattgcag gctggtggtg
    63421 atggtattgt cctgcaaggt ggtcagacgg aacatctgcc ccattgctga tgcaccttca
    63481 acttgctgtt tcgccagttt tttcgcttga tcttcctgat aaccttccag cttcgctacc
    63541 tgagtcatca actcagttgc catatccacc ggaatggtca gtttcgcatc cagagattta
    63601 accgaacggt ctacttcctg cgccagcgtt tgcggcgctt ctttagtcgt tgccggatct
    63661 ttcaggaaca gcgacagatt cagcgcactt tcaccctgac tgtttttcca gcttagcggc
    63721 gcgatagtaa tcaccggatc gcctttcaac atcagcggca gggcgctaaa gaaggcttcc
    63781 gtcacttttt cctgataaag ttcgggattg ttggcaattt ctggctgtgc cagcagcgcc
    63841 tgagtttgcg cgttatattg ctggctaaac tgatgccagg cttcgccatc aatttggccg
    63901 acttttaaag tcagcttgcc gctgcccaga tcctgattct gtaccttcag gctgtttagc
    63961 gagtaatcca gttggctatt gatcgtttta ccatcattga ccagatccga tttaccgctg
    64021 atctccatgc cttccagcag tgccagttct ttgccttcca ctgaaatggt cattttttcc
    64081 agtgacagtt tttggtttcc tacgcgctca ccaaaacttg ccagcgtgct ggaaccgtcg
    64141 gttttcagat tattaaaggt caactgtact ttctggttgt attcgttaac agcgtctatc
    64201 cgaccacttt gcgcctcccc ggaaagggag atagctttgc cgtctctgtc tgcatttaac
    64261 tggaactcgc cgccgctaaa ggcgactttt tcatcctttt gctcgtaatt cagtggcttg
    64321 agcgaaatgt cggaactgga atctccgctg taaccaatgc gggagttaat ctcaaaaggc
    64381 gtttcacctt ttgccatatc aaacagtggt ttgcttactt cgttattaac cagcgtggtt
    64441 tgaattgatg ccatcgacgg gatcagattc agttttttaa gctgggcaag cgggaaggga
    64501 ccatgatcaa ccgattcgtt gaagatgacg ctctgaccgc ttttaatcca cggatttact
    64561 tttccggcaa tgggtttcac caacagttgc agctgactgc tgaatacgcc gcgatgatag
    64621 ttttgataac tcacttccag gttggattca ggagcggtca gtttgagttg cgcgttcgcc
    64681 tgcgcgacca tgtcttcgag atgggtttca atcttcttgc ctgtatacca tgcgccgcct
    64741 gtccagacta cgcctagcgc aacaatgacg cctaccgcta ccagcgattt attcataatg
    64801 attatccata aaatgaaatc aggcggactg gccgcctgaa ggtgttataa gcctttaata
    64861 agcttagcaa gagatgttaa ttttttcagt aagctcttac agcttgttgt aaacacgcgc
    64921 taaacggccg tggcctctga cagtcaccgg tgattcgttg gcggcaataa acgctgattc
    64981 acccggttta agctgtaact gctgagaacc tttacacagc gttgcatcgc cttcgacgca
    65041 gaacaaaatg gcggcactct gctggctgat ggtggtttct ttatcactaa ggtcatgcaa
    65101 cgagaaggca aaatcatcca caggaatcgg gaagtccagt tctgcacctt gttttaccgg
    65161 ctgggtcaac aactggttag ccggtttggc ttcgaacttc acgttggcaa ccagttccgg
    65221 aatatcaatg tatttaggcg tcagacctgc acgcagcacg ttatcggagt tcgccatcac
    65281 ttccagcgcc acgccttgca ggtaagcgtg cggtgtttca gcgaacagga acatcgcttc
    65341 gccaggattc aatttcacca cattcagcaa cagcggggag aacaggccgc tgtcttccgg
    65401 gtaacattca gaaattaaac gaatcgtttg ccatggttca ccctgctggc tgtcgagggc
    65461 cgattttaaa atcgccagcg cgcgggattt ttcttcaccc tgcatattca acaggctggc
    65521 gaacagttcg cttaaacgtt cggcatcagg ctgttgtaaa aagtgagcaa tcgccggatg
    65581 tgcacctgcg actggctgga gcagggagac aatctcggaa aattcacgaa acgcgttcat
    65641 cgcaaggaaa ggcgtcagcg caaaaaccag ctccggcttg tggttaggat ctttatagtt
    65701 acgctcggcg gcatccatcg gaatacctgc ggcattttct ttggcaaaac cgatttcaga
    65761 attgcgtttg tttggatgaa cctgaatgga gagcggctgt gctgcgcata atactttgaa
    65821 caggaaaggc agttcgccaa agcgtttggc aacggcctct ccgagcagag tcgatttatt
    65881 actctcaatc acatcacgca gtgaaacgat atctccggcg gcattctgca ctcgtgaact
    65941 gctttttgga tgtgcgccca tccacagctc ggccatcggc tggctggacg gattttccat
    66001 accataaagt tcagtcaacg ccgttttgct gccccaggca tagttttgca ctgagttaat
    66061 gagtttttgc atgatcaatc cctgttttaa tgtggaaatt aatcccgcta ttaaagcaag
    66121 aatcctacgg gaagtaacct ggagccgcaa aaagtcgtac tagtctcagt ttttgttaaa
    66181 aaagtgtgta ggatattgtt actcgctttt aacagggcaa cggaacaccc gcccagagca
    66241 taaccaaacc aggcagtaag tgagagaaca atgtcaaaca aaccctttca ttatcaggct
    66301 ccttttccac tcaaaaaaga tgatactgag tattacctgc taaccagcga acacgttagc
    66361 gtatctgaat ttgaagggca ggagattttg aaagtcgcac ccgaagcgtt aactttgttg
    66421 gcgcgtcagg cgtttcatga tgcgtcattt atgctgcgtc cggctcacca acaacaggtg
    66481 gccgacattc tgcgtgaccc ggaagccagc gaaaatgata aatatgtggc gctgcaattc
    66541 ctgcgcaact ccgacatcgc ggcgaaaggc gttctgccaa cctgtcagga taccggcacc
    66601 gcgattattg ttggcaaaaa agggcagcgt gtatggaccg gtggcggtga tgaagcggcg
    66661 ctggcacgcg gtgtctataa cacttatatc gaagataatc tgcgctactc gcaaaacgcg
    66721 ccgctggata tgtataaaga ggtgaatacc ggcaccaatc taccagcgca gatcgatctt
    66781 tatgccgttg atggcgacga gtacaaattc ctctgtatcg ccaaaggtgg tggttcggca
    66841 aacaagacgt atctctatca ggaaaccaaa gcgttactaa cgccggggaa actgaaaaat
    66901 tacctggttg agaagatgcg cacgctgggt acggcggcct gtcctccgta tcatattgcg
    66961 ttcgtaattg gtggaacttc tgcggaaacg aaccttaaaa cggtgaaact ggcttccgct
    67021 aaatactatg acgaactgcc aacggaaggg aatgagcacg gtcaggcgtt ccgcgatgtg
    67081 gaactggaaa aagaattgct gatcgaagcg caaaatcttg gtctgggtgc gcagtttggt
    67141 ggtaaatact tcgctcacga catccgcgtg attcgcctgc cacgtcacgg cgcatcctgc
    67201 ccggtcggta tgggcgtctc ctgctctgct gaccgtaata tcaaagcgaa gatcaaccgt
    67261 caggggatct ggatcgaaaa actggaacat aatccaggca aatatatccc ggaagagctg
    67321 cgtaaagcgg gagaaggcga agcggtgcgc gttgacctta accgtccgat gaaagagatc
    67381 ctcgcacagt tgtcgcagta tcccgtttct acacgcttat cgctgaatgg cacgattatc
    67441 gttggtcgtg atattgctca cgccaaactg aaagagcgga tggataacgg cgaagggctg
    67501 ccgcagtata ttaaagatca tccgatttac tacgcgggtc cggccaaaac gccggaaggt
    67561 tatgcctccg gttctcttgg cccaacgacc gccggacgga tggattctta tgtcgatcaa
    67621 ctgcaagcgc agggcggaag tatgatcatg ctggcgaaag gcaaccgcag ccagcaggtg
    67681 acggatgcct gtaaaaaaca cggcggcttc taccttggca gtatcggtgg tccggcagct
    67741 gtattggcgc agggaagcat taagagcctg gaatgtgttg aatatccgga actgggaatg
    67801 gaagccatct ggaaaattga agtggaagat ttcccggcat ttatccttgt ggatgataaa
    67861 ggaaatgact tcttccagca gatacaactc acacagtgca cccgctgtgt gaaataaaca
    67921 gagccgccct tcggggcggt ttttttacat ggcacgaaag accaaacatt tgttatcaaa
    67981 tggtaaataa tgagtaagct aaaagttgct taacgaaagc aaaacagaaa gaaaaaatta
    68041 atcaggtgag gagcaggcca tgaatacagt acgcagcgaa aaagattcga tgggagcgat
    68101 tgatgtcccg gcagataagc tgtggggcgc acaaactcag cgctcgctgg agcatttccg
    68161 catttcgacg gagaaaatgc ccacctcact gattcatgcg ctggcgctaa ccaagcgcgc
    68221 agcggcaaaa gttaatgaag atttaggctt gttgtctgaa gagaaagcga gcgccattcg
    68281 gcaggcggcg gatgaagtac tggcaggaca gcatgacgac gaattcccgc tggctatctg
    68341 gcagaccggc tccggcacgc aaagtaacat gaacatgaac gaagtgctgg ctaaccgggc
    68401 cagtgaatta ctcggtggcg tgcgcgggat ggaacgtaaa gttcacccta acgacgacgt
    68461 gaacaaaagc caaagttcca acgatgtctt tccgacggcg atgcacgttg cggcactact
    68521 ggcgctgcgc aagcaactca ttccacaact taaaaccctg acccagacgc tgagtgaaaa
    68581 atcccgcgta tttgctgata tcgtcaaaat cggtcgtacc cacttgcagg acgccacgcc
    68641 gttaacgctg gggcaggaga tttccggctg ggtagcgatg ctcgagcata atctgaaaca
    68701 tatcgaatac agcctgccgc atgtagcgga actggctctt ggcggtacag cggtgggtac
    68761 tggactaaat acccatccgg agtatgcgcg tcgcgtggca gatgaactgg cagtcattac
    68821 ttgtgcaccg tttgttaccg cgccgaacaa atttgaagcg ctggcgacct gtgatgccct
    68881 ggttcaggcg cacggcgcgt tgaaagggtt ggctgcgtca ctgatgaaaa ttgccaatga
    68941 tgtccgctgg ctggcctctg gcccgcgctg cggaattggt gaaatctcaa tcccggaaaa
    69001 tgagccgggt agctcaatca tgccggggaa agtgaacccg acgcagtgtg aagcattaac
    69061 catgctctgc tgtcaggtga tggggaacga tgtggcgatc aacatgggtg gcgcttccgg
    69121 taactttgaa ctgaacgtct tccgtccgat ggtgatccat aatttcctgc aatcggtgcg
    69181 cttgctggca gatggcatgg aaagttttaa caaacactgc gcagtgggca ttgaaccgaa
    69241 tcgtgagcga atcaatcaat tactcaatga atcgctgatg ctggtgactg cgcttaacac
    69301 ccacattggt tatgacaaag ccgccgagat cgccaaaaaa gcgcataaag aagggctgac
    69361 cttaaaagct gcggcccttg cgctggggta tcttagcgaa gccgagtttg acagctgggt
    69421 acggccagaa cagatggtcg gcagtatgaa agccgggggt taatctgcaa catacaggtg
    69481 cagccgtgga atgatcaaac gaagcggctg cgcctgtggt ttataacggt gttgcacatt
    69541 gtcggcatcg taatttaaca actccccaac gtccggcacg cccgcgccat tatcccgatt
    69601 aatcagcgca ataagcggtg tagggcaagc gtgttgtact tgtttttgat ctcccttgta
    69661 ccagacgcgg gcaatcggct gcaccttcac cggacgtttg atttttaact tcgcgtcctg
    69721 tggcagggta gcaatatcct gatactctct ctccagtttt ctttgccact cctcgcgcgt
    69781 ccacggtgcg acactgcgcg gtgatttcag gcttttttcc agctgtgcca ggacttcatc
    69841 acgatgcaaa ttcttaatga tatgtttatt ggcccagcca aaacgtaaag tggcgggatc
    69901 gtgcagaaca gtgagcgttc ggtaagcatt aagggtgatc agccccggca aatgacgatg
    69961 cacccattca aaacgcgccg ctgtggggag ttctgactca accgtgacga tatgctcgaa
    70021 cgtagtcttg agttgattga tgtgctgaat atgactgacc aacgctgctt gcgaaaggtt
    70081 atcgacctgg taacacaaca cgccaggtag acgaacagcg gtcttgctgc tgcgattttc
    70141 ggactgttgt tgaataaata aatggcggaa atgacgtaac gccagcgact gcgcgtcgtt
    70201 gccgagatgt tgctttacct caatacgatt aagcggatta tgctcatcct ctttttttac
    70261 ctccggcaaa gagaacacgc gggcaaccag caatttgtgc tgttctagct cagcggcaaa
    70321 tgcagccagc tcttgttcca tctggcgaaa ggtagtgttg agtcggtcta cgagatcgta
    70381 acgcgccata atattaacca ctttagttac aacatcctta ttttatagca cagtggtggt
    70441 gacttgacca ccgtgcgcgt aatgtcaagc agaggtaaat tgcgggatgt tatgccataa
    70501 cggccagcta aacgagaagc gggcaccacc cagttcgctg gtgtcacaat taaccgtacc
    70561 gcccattgcc agtgctatag agtggacaat tgccagcccc agcccgcagc cgccggttga
    70621 gcgatcccgg ctgggatcga ggcgaacaaa aggttcaaag atatgttcgc ggttttcagg
    70681 ggcaatccct gggccatcat cctcaacaat taatgtcgct ctattccccg acagtagcag
    70741 gctggtttca accgttgagt gacagtagcg cagggcgtta ttgagcaaat tatccagcac
    70801 gcgctccatt aagcgcatat ccaacgcagc ataatggcct tgcgcgagcg tttttatccg
    70861 taccgtttta tcgggcgtta ctgcctgaat atctgccaga tgcgttgaca gccacaacgg
    70921 caggtctggt tcgctaagat gaagctcatt ttgtgggcga tcgagtcggg cataagtcag
    70981 cagctcttca attaaagctt caagttgact gatatcgcga ttcaacgcct gggattcgac
    71041 ggcgctcagg ttatcgctca tctccagtcg ataacgcagg cgcactaacg gtgttcgcag
    71101 ttcgtgagcg ataccgtcaa taagctgttt tttgctggca atcaaggcgt tgatattgtc
    71161 cgccatctgg ttaaatgcga cgccaagtcg ttcaaagctc gaaccttcat caaagtggat
    71221 acgttcattg agatgcccat cgccaaatcg ttgcgccgct gcttccagtt ttaacatatc
    71281 ctgccagtgc ggacgcatcc aaataaacac cggaaaggcg agggaaatgg caataaaagc
    71341 gatcagggcg atatccagta atcgcatctg atggaggtaa taaagataag gaacaggacc
    71401 aactgccagc acgtagtggc tgcgcgggat acgttgcaaa aaagtgtact gatcgtccag
    71461 ggcgacaatt tcgccgccac gcagccggtg catggaaata tcatcaagat ggtatttact
    71521 cagtggctcg actcgcagat cgaaagagag atttaaatcc atctccttca gcgtcttacc
    71581 ccaatcgtgt ggggggatct cacgcaattc gctgcgcatc agatacagcg aactgttcat
    71641 caaatcatcc agcgactgtt tgcccgcgcg ttcggcggta aatttgtaca ccagcccaac
    71701 cagcagagac atcacaagga agcagacaaa caataacagg taaaactgga taaacagttt
    71761 tttcatcgct tattcccatg catgaggcgc aaaaagatag cctttgttac gaacagtttt
    71821 aatgcgataa ggttctgcgg cgttatcgag cagttttttt cttaaccgcg aaatagccac
    71881 gtccacgcta cgatccagtc cgtcataact gacgccgcgt aaatttttca gcaatgcatc
    71941 gcggtccatg atttgcccag catgggtagc taattcccac aataattcga aatcagctgt
    72001 cgagagcgag atttcagtgt tagccagggt gactacgcgg ttgataggat cgatggtcaa
    72061 cgtgccgaaa tgcagggctt tgtagggagt cagagacgtt tcctgaagac ctttgctcag
    72121 tgtggcttgc tcattctgac gcaaatgcaa acgtaaacgc gctaacaaaa cagcaggggg
    72181 cgtcgttttg agaatatagt cgcaggcacc catttccagt gccaggatgt ggttcatatc
    72241 gctatcgaga gaggttagaa gaacaatcgg tccggaccac tttgcgcgta aatcacgaca
    72301 aatggtcatg ccgtccttgc ctggtagcat gatgtcgagt aacaccaaat ccggattttc
    72361 ttgcagaata gtttcttcgg cccggtcgcc gcgcggttcc acggtaacct gcatatcatg
    72421 ttttgccagg tacgcggcaa tcagtgaacc gacttccgca tcatcttcca caaatacgat
    72481 agtgttcata acattcacgg tagatataaa aacgtcacaa tacaccgcgc tgtttttact
    72541 tactatcaat cgtttctaaa caaggtttca tcaggtattc tactgataac ttgttatgga
    72601 agtgttaagg taaaaagatg gggctggtaa tcaaagccgc gctcggtgcg ctggtagtgc
    72661 tgttgattgg tgttttagca aaaacgaaaa attattatat cgccgggctg ataccacttt
    72721 tcccgacctt tgcgctcatc gcgcattata ttgttgccag cgaacgcggc attgaagcct
    72781 tacgcgcaac catcattttt agcatgtggt cgattattcc ctattttgtt tacctggcgt
    72841 cgctgtggta tttcaccgga atgatgcgct tgcccgccgc ttttgttggt tctgttgcat
    72901 gctgggggat aagcgcatgg gtgttgatta tatgctggat aaagctgcat taacgcagat
    72961 gacgaccgcc atcaagaggg aaactgcgtc cggtgacaaa gcagctggta agtaagtaat
    73021 cgaccaggtc gatcacttct ttctcgccag gcgcggtttt catcagtgat ttattcagcg
    73081 cctgttgtcg gtattcggca tcatcatgct cattaaacag gatcagcgat ggcgcaatag
    73141 aattcacttt cacttccggt gccagcttgc gggcaaacga gcgggtcata ttatccagtg
    73201 ccgctttgct tgcagcatag gcaatatgtt tgtcgctacc gcgctccacc acataatcgg
    73261 taaagtgaat gatatcgctg gcggcgtgtc cgtgcccacg cagtaatctt tccagtgcat
    73321 ggttgagtag gtatggggta ttaacgtgga tctgcatcat gcaagccaga acgtcggtca
    73381 gtggtgtacc tggtttttct gccatccacg cgctggcgtt atgcaaaata gcacgcaggc
    73441 catgggtgct ttttagtact tcatcggcaa acgccatcac accgtcgttg gtcgaaaaat
    73501 cagcctgaat acactgcgca cctgcgttaa tcagtccatc aatggcggga tagtgcgtcc
    73561 gatagctgat aatcaccggt tgcttttgat taatgaaatg ccaggcgagg gcgaggccga
    73621 tgcgacgacc tccgccagta attaatattg gcaagggttg ggctttaccc atcgttatct
    73681 cctttgctat ccaacgatgg gatgaacgtt atcccaccag catccaggtc gccggaactg
    73741 aggcaattaa cagcatgcca atcagcacca tttcctgacg attcagtaca ttatcatggg
    73801 tatgcgtttt acgcgcgtac aggaaaacca gcagccccgg tgcatacaga acaacggata
    73861 acagcaagtg cattggacca gaggcgtaca ataaccataa gccataaatg caggcaccga
    73921 cacctaccgc tttatgcagt ggacgggttg cgattttcag caagaacgcg ccgaccagga
    73981 aatagggcac cagaatcatt tctgaggcga tggtcagcaa tgtgttgtaa tcggaaccgg
    74041 tcagccagat gagtaccaga caaatttgca cgcagatatt ggtcagccac agtgaagcgg
    74101 atggcgcagc ttgtgcgttc tggcgcgcaa aaatgcgcgg gaatgcttta tgagtggctg
    74161 ccaggaacgg aacttctgcc gccatgatgg tccagcttaa gtacgcaccg caaacggaaa
    74221 cgatcaaacc ggcagcgatg atgatttcgc cccacggtcc catcatttcc accatcagtc
    74281 cggccattga cgggttacga atttcagcca gttcaggacg tgccaccacg cccagcgaaa
    74341 gcagcgttac cagcaagtaa acgcccagag cggagagaac cgccagcagt gtcgctttgc
    74401 caacatcacg tttattacgc gcacgcgcag aaacaaccac ggcaccttcc acaccaatga
    74461 aaacccacag ggtgatcagc atggtgtttt tcacctgttc ccaaacgggt acgccaagtg
    74521 caagtccggt gaagtcgagc ttgaaggtat ccagtttgaa catcatcatc gccagcacaa
    74581 caaacagacc cagcggcaac aattttgcca gtgtcgccac caggttaatg ctggcagcgg
    74641 tttgcactcc gcgcaggatc aaaaagtgaa caatccacaa taaagccgat gcaccgacga
    74701 tcgattgcca ggtattacca tcgccaaaca ggcgcaattc cggcgtgtcc gtaaaaaagc
    74761 ttaacgcgga aaaaacgatc accagatagg agacgttggc gatgaccgcg cacagccagt
    74821 atccccatgc ggaacaaaag ccgattagct caccaaatcc ttcgcgggca taggtaaaga
    74881 taccgccgtc aagttcggga cgaatgcgcg tgaggatcag catggcaaag gtcagtaata
    74941 aaataccagc accagtaata ctccagccga tgagcagtgc tgccgggctg gcaactgccg
    75001 ccatattttg cggcagactg aaaacacccg cgcccagcat tgagcttaat accagcgcgg
    75061 tgagtgcgct cagtcccagt ttcttttcca tcgatatcca gtgattaaaa cgtgacaaga
    75121 agaataaata ttagggcaaa acgcataaaa atggagtttg ccactgaagc gcgggatttt
    75181 acgaagagat gtgtctgcat gcaatggtcg cggggaaatt tgtgactaaa aatgcgaaag
    75241 gcggcatcaa gccgccttat aggagtaact gaatagttat ttatacagat ctgcgctgac
    75301 ggtcaggtta ttaccacgtt cttgccactg gcgggtgatg tggtaatact tagcaccttt
    75361 cttcgcggca cgtttcgcaa cctgatagga gacttcagtc atgttgccgt agttgccaga
    75421 gaatttgatg ctgtcgaacg ggaccatcat cgctgcggtc gctttgttca gttcttcgac
    75481 tttagtgccg tctgggagcg tgacagtgta acgcccgcct tttgatgact gggtttcaaa
    75541 gaagcgaccg acttcagaac ttggtgatgc ggtagtcgca acacccggga tctcaacttt
    75601 cttcgcagct tcgccaccgg cagccagagc tgcacgtcct gcttcggaat ctgccgggat
    75661 cacatccggg ctctggacga tacgtttctt agcatctttt ttatagatga atgcagtaat
    75721 acgctggttg ccgccctggt tggcatcgat ttgacgaacg atgtagaaag agtaggcacc
    75781 tttcgctttt gccgctttgg tgatggcatc attgacttct ggctggctac gatagaagcc
    75841 ctggacggtg accgtgtcaa acggttcaat cagaacagcc tgatctttcg gcagttcgac
    75901 aacaccgtta attacgcgat tacttgtttc ttctgctttt tcagcatcgg ctttatagag
    75961 gtcagcgacc acacgccagt taccgctgtt accaaaatca gaagtgtcga caacataaaa
    76021 agaggcggca ccttctttat cagcgcgacg agaaacggct ttcaccgctt cgccaatagc
    76081 attaaaacga ccggtaacca ctacacggtc aaaaggttta accgctgccg cttgctccgg
    76141 tgtcagttct gttgctgcgt taacggagaa tgccgtagca gaaagcagtg ccgacgccag
    76201 gagggtgttc ttaagcttca taaaaataat ccttcgcctt gcgcaaacca ggtactggta
    76261 ttgttattaa cgagaaacgt ggctgattat tgcatttaaa cggtgtaact gtctgcgtca
    76321 tttttcatat cacattcctt aagccaattt taatcctgct caaatggccg tctatgctta
    76381 aaaaacagcc gtatcagcat caatactact gaagcaactg aattgtataa gttaatttaa
    76441 tgttaagtag tgattcgtgc cggggcgatg tctcgtttta tgcgaacgcc gaagacaatt
    76501 atcagtcttt atccggcgtt ctaaggtgtt tatcccacta tcacggctga atcgttaata
    76561 ttttgcgagt tcacgccgaa atactgattt ttggcgctag atcacaggca taattttcag
    76621 tacgttatag ggcgtttgtt actaatttat tttaacggag taacatttag ctcgtacatg
    76681 agcggcttgt gtggctcctg acacaggcaa accatcatca ataaaaccga tggaagggaa
    76741 tatcatgcga attggcatac caagagaacg gttaaccaat gaaacccgtg ttgcagcaac
    76801 gccaaaaaca gtggaacagc tgctgaaact gggttttacc gtcgcggtag agagcggcgc
    76861 gggtcaactg gcaagttttg acgataaagc gtttgtgcaa gcgggcgctg aaattgtaga
    76921 agggaatagc gtctggcagt cagagatcat tctgaaggtc aatgcgccgt tagatgatga
    76981 aattgcgtta ctgaatcctg ggacaacgct ggtgagtttt atctggcctg cgcagaatcc
    77041 ggaattaatg caaaaacttg cggaacgtaa cgtgaccgtg atggcgatgg actctgtgcc
    77101 gcgtatctca cgcgcacaat cgctggacgc actaagctcg atggcgaata tcgccggtta
    77161 tcgcgccatt gttgaagcgg cacatgaatt tgggcgcttc tttaccgggc aaattactgc
    77221 ggccgggaaa gtgccaccgg caaaagtgat ggtgattggt gcgggtgttg cgggtctggc
    77281 cgccattggc gctgcaaaca gtctcggcgc gattgtgcgt gcattcgaca cccgcccgga
    77341 agtgaaagaa caagttcaaa gtatgggcgc ggaattcctc gagctggatt ttaaagagga
    77401 agcgggcagc ggcgatggct atgccaaagt gatgtcggac gcgttcatca aagcggaaat
    77461 ggaactcttt accgcccagg caaaagaggt cgatatcatt gtcaccaccg cgcttattcc
    77521 aggcaaacca gcgccgaagc taattacccg tgaaatggtt gactccatga aggcgggtag
    77581 tgtgattgtc gacctggcag cccaaaacgg cggcaactgt gaatacaccg tgccgggtga
    77641 aatcttcact acggaaaatg gtgtcaaagt gattggttat accgatcttc cgggccgtct
    77701 gccgacgcaa tcctcacagc tttacggtac taacctcgtt aatctgctga aactgttgtg
    77761 caaagagaaa gacggcaata tcactgttga ttttgatgat gtggtgattc gcggcgtgac
    77821 cgtgatccgt gcgggcgaaa ttacctggcc ggcaccgccg attcaggtat cagctcagcc
    77881 gcaggcggca caaaaagcgg caccggaagt gaaaaccgag gaaaaatgtg cctgctcacc
    77941 gtggcgtaaa tacgcgttga tggcgctggc aatcattctt ttcggctggt tggcaagcgt
    78001 tgcgccgaaa gagtttcttg ggcacttcac cgtttttgcg ctggcctgtg ttgtcggtta
    78061 ttacgtggtg tggaatgtat cgcacgcgct gcatacaccg ttgatgtcgg tcaccaacgc
    78121 gatttcaggg attattgttg tcggagcact gttgcagatt ggccagggcg gctgggttag
    78181 cttccttagt tttatcgcgg tgcttatagc cagcattaat attttcggtg gcttcaccgt
    78241 gactcagcgc atgctgaaaa tgttccgcaa aaattaaggg gtaacatatg tctggaggat
    78301 tagttacagc tgcatacatt gttgccgcga tcctgtttat cttcagtctg gccggtcttt
    78361 cgaaacatga aacgtctcgc cagggtaaca acttcggtat cgccgggatg gcgattgcgt
    78421 taatcgcaac cattttggga ccggatacgg gtaatgttgg ctggatcttg ctggcgatgg
    78481 tcattggtgg ggcaattggt atccggctgg cgaagaaagt tgaaatgacc gaaatgccag
    78541 aactggtggc gatcctgcac agcttcgtgg gtctggcggc agtgctggtt ggctttaaca
    78601 gctatctgca tcatgacgcg ggaatggcac cgattctggt caatattcac ctgacggaag
    78661 tgttcctcgg tatcttcatc ggggcggtaa cgttcacggg ttcggtggtg gcgttcggca
    78721 aactgtgtgg caagatttcg tctaaaccgt tgatgctgcc aaaccgtcac aaaatgaacc
    78781 tggcggctct ggtcgtttcc ttcctgctgc tgattgtatt tgttcgcacg gacagcgtcg
    78841 gcctgcaagt gctggcattg ctgataatga ccgccattgc gctggtattc ggctggcatt
    78901 tagtcgcctc catcggtggt gcagatatgc cagtggtggt gtcgatgctg aactcgtact
    78961 ccggctgggc ggctgcggct gcgggcttta tgctcagcaa cgatctgctg attgtgaccg
    79021 gtgcgctggt cggttcttcg ggcgctatcc tttcttacat tatgtgtaag gcgatgaacc
    79081 gttcctttat cagcgttatt gcgggtggtt tcggcaccga cggctcttct actggcgatg
    79141 atcaggaagt gggcgagcac cgcgaaatca ccgcagaaga gacagcggaa ctgctgaaaa
    79201 actcccattc agtgatcatt actccggggt acggcatggc agtcgcgcag gcgcaatatc
    79261 ctgtcgctga aattaccgag aaactgcgcg ctcgtggtat caacgtgcgt ttcggtatcc
    79321 acccggttgc ggggcgtttg cctggacata tgaacgtatt gctggctgaa gcaaaagtac
    79381 cgtatgacat cgtgctggaa atggacgaga tcaacgatga ctttgctgat accgataccg
    79441 tactggtgat tggtgctaac gatacggtta acccggcggc gcaggatgat ccgaagagtc
    79501 ctattgctgg tatgcctgtg ctggaagtgt ggaaagcgca gaacgtgatt gtctttaaac
    79561 gttcgatgaa cactggctat gctggtgtgc aaaacccgct gttcttcaag gaaaacaccc
    79621 acatgctgtt tggtgacgcc aaagccagcg tggatgcaat cctgaaagct ctgtaaccct
    79681 gacggcctct gcggaggccg tcactcttta ttgagatcgc ttaacagaac ggcgatgctt
    79741 tgacctcccg ctgtttgttc aagcgcaatt ttgacaataa ttgtcaacgg cacggaaagc
    79801 agcataccca ccggtcctaa caaccatccc caaaaaatca acgacaaaaa taccaccaat
    79861 gtcgaaagcc ccagcccacg tcccatgatg cgcggctcca gaatattgcc gaagaccaga
    79921 ttaatcagca gatatcccgc cagcaccagc aacgcttcgt agaagccatt aaacaccagt
    79981 acctgagcaa taggggggat tgccgcgaga actgaaccaa tattcgggat gtaattaagc
    80041 gcaaaggcca gcaatcccca gacaaaagcg aagcgaacat cgagtgcggc gagcatcgcc
    80101 catgcgacca ggccggtgat gatgctgatg gctgttttca gcaccagata atgagacaca
    80161 ctgtcaatcg cacgctgaat cgccgccatc ccttcaaccg gacgcgccat catttgctga
    80221 aattttccgg gcaattgtgg cacttcgagc agcataaaca gcaccgtcag cagcaataaa
    80281 aagattgatg acatggcatt agataactgc gtcaataagt tggtgagcaa cgtcatcgcc
    80341 gcgttcggat caatgtaatg cgccagttgg tcaactgaga catcaatccc tacgcgttgc
    80401 aacaacggtt caatcgcttt cagtggcgtc ataatagagt tgcgatattg ctgtaacgtc
    80461 cgcgtcaact cattgagcgc ggaacccaga taagctaaca gcaacaccat cgccatcacg
    80521 atgatggtca tcaaaattga cactgccagt acacgcggca cacgccagcg gaccatgtgt
    80581 tgcaccagcg ggttaagaat aacagcaata aataatgcga gaataaacgg cacgatgatc
    80641 tcggcggcaa aacggatacc gcagagaata atgaccagca ttcccaacat aatgacgatt
    80701 tttaggccat tgagcgtgat gatcggcttt gccatgcttg tccccgtttt ttcttccttt
    80761 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    80821 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    80881 ccgaggacaa ttatcatccg cgatgacgag aagcaacact gcggataatt gtaatattat
    80941 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    81001 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgccaaaact
    81061 tgcgctaagc gcgggcttga ccactttact ttggtttcgc tgaattaagc gaaaattaaa
    81121 ataattctct tgcaggagaa ggacaatgta tatttattgg attttattag gtctggctat
    81181 tgctacagaa attaccggta cgctgtccat gaaatgggcg agcgtcagtg agggaaatgg
    81241 cggctttatt ttaatgctgg tgatgatttc tctgtcgtat atatttctct ctttcgccgt
    81301 taaaaagatc gccttaggcg tagcttatgc gctgtgggaa ggtatcggta ttttatttat
    81361 taccttgttt agcgttttgt tattcgacga aagtttatct ctgatgaaaa ttgccgggtt
    81421 aaccaccctg gtcgccggga ttgtgttgat caaatcaggt acccgtaaag cgcgtaaacc
    81481 tgaactggag gtgaaccatg gcgcagtttg aatgggttca cgccgcctgg ctggcattgg
    81541 caatcgtgct ggaaatcgtt gctaacgtct ttttgaaatt ttctgacggc tttcgtcgca
    81601 aaatatttgg cttgctctct ctggcggcgg tgctggctgc ctttagtgcg ctttctcagg
    81661 ccgttaaagg gatcgacttg tctgtcgctt atgcattgtg gggcgggttt ggtattgccg
    81721 ccacgttagc cgcaggttgg atcttgtttg gtcaacggtt aaatcgtaaa ggctggattg
    81781 gcctggtgct gctgttggct ggaatgatca tggtgaaact tgcctgatga acatgctgcc
    81841 cgcgctgtcg ggcagcgttt gaacattatt tttgcgacag ttgatccagc ttgtcgcgaa
    81901 aaccggtaac agaaatggcc cggttatcgg cgcgccagcg atctttcgcg gcaggagccg
    81961 aactttgcac cccaattaat tgccagccgt catcggtatg caacatcaga ggcgaaccgc
    82021 tgtcacccgg caaggtatcg cactgatgcg acatcaccga cgtttgcgcc cagccagtca
    82081 cttcacagtt ttgatgactg tacaacgtat cgagatgatc ttcagggtat cccgcctgag
    82141 tcactttacg acctgccgct tttaatgcgg cggtaagcgc ggctttatct ccctcaaata
    82201 acggcaatgg tgtaatgcca gaagggggat tacgtagcac aatcaaaccg aagtcccacg
    82261 gcgcagctgc gggaggaaca atccaaccat ccccatctgc ttttaaccgc tttcccagtg
    82321 tcgggtcaac gcggccttct atgtcgtgga tctcatagcg ccaaagacct ttatttgaca
    82381 caaaacgcag cgccaccgct ttatcggctt taccctttgg cggcgtcaat aaacagtgtc
    82441 ctgccgttaa tgccagattg ggtgcaatca gcgtcgctgt acataaattg ccgctggccg
    82501 tttccagttg gccaaccgca tcccacggtg attgggtcgt gtcattcact ggcacacgat
    82561 catcatgacc aaaaaacagg gtgctgacct catcgtttgc cgatttggca acgtctggtt
    82621 tatctgcaaa cacaaaagca gacgtcaaac taattgcacc caacactaca gcaatggttc
    82681 tacgcatatc acactctggt gggtaattat gattattaaa agcaaaccct cataaatact
    82741 atagacggga cgggatgaaa gtgggagtaa aatcagataa ctacgatcag gaaagataaa
    82801 aacgggcggc aatcagtgcg cataaaataa ggatgataag aatgaactca aagcgataac
    82861 ggcccaccat actaccctcc gctaaaggcg gtgctaaacg ttacccgttt agcaccgtaa
    82921 ttttcaggcg cgagggggcg cgccagcatt actgttgaaa acttacgctg cgggttgtgc
    82981 agcaggtttt gccggttgct gatggctgtg tttcttggcg tgtttcttcg ctgcctgcgc
    83041 tttttgttca ggagctttct gttcagcttt ggcattttta tgatgctttt tagccgcctg
    83101 cgctttctgg gcaggtgctg ctttatgctg ttttttatga tgtgtggttt tcgcaggcgc
    83161 tgctttggtg gtcgtcgcag tcggagcagg tgtggtcgca gtctctgcag caaaggcagc
    83221 agaagacaga cccatagcag cggcaacaac cagagctaat acttttttca ttgtcatacc
    83281 ctcaatttgt tttttcattt aaccccactg cggggccgtt gaaataacta tatccctgag
    83341 aatggcagac ttccgtgagt ggttggtttc agcgtgtaac gatatgtaca aacgctgaat
    83401 aaattacagc gttgataata acgtattgtg acttaaggaa aattcatcta ccaataatag
    83461 tagtcttgat cgtgttaact attttactac ttacaaagcg gtagaataac tgcgcatcag
    83521 taataaatga cacacagcaa aatgaatccg tttatttggg tacttataat cctgatgaca
    83581 ctagatgcgc tgcgggaatt ggctggcgct tcgtctattt taggatggct attaacgttg
    83641 gtttgagcag gcaataagtc cggacgggta tttacctcag tccggactt
//
LOCUS       DAZGHF010000005        74208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGHF010000005 DAZGHF010000000
VERSION     DAZGHF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.005"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(21..584)
                     /gene="pabA"
                     /locus_tag="ACHK3A_000392"
     CDS             complement(21..584)
                     /gene="pabA"
                     /locus_tag="ACHK3A_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO8738535.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(616..1218)
                     /gene="fic"
                     /locus_tag="ACHK3A_000393"
     CDS             complement(616..1218)
                     /gene="fic"
                     /locus_tag="ACHK3A_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO8738536.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(1208..1375)
                     /gene="yhfG"
                     /locus_tag="ACHK3A_000394"
     CDS             complement(1208..1375)
                     /gene="yhfG"
                     /locus_tag="ACHK3A_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO8738537.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(1480..2052)
                     /gene="ppiA"
                     /locus_tag="ACHK3A_000395"
                     /gene_synonym="rotA"
     CDS             complement(1480..2052)
                     /gene="ppiA"
                     /locus_tag="ACHK3A_000395"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO8738538.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            2323..3504
                     /gene="tsgA"
                     /locus_tag="ACHK3A_000396"
     CDS             2323..3504
                     /gene="tsgA"
                     /locus_tag="ACHK3A_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO8738539.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILIV
                     LAGLAAILMYVFNTGTPAYMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            3766..6309
                     /gene="nirB"
                     /locus_tag="ACHK3A_000397"
     CDS             3766..6309
                     /gene="nirB"
                     /locus_tag="ACHK3A_000397"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO8738540.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHANLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            6306..6632
                     /gene="nirD"
                     /locus_tag="ACHK3A_000398"
     CDS             6306..6632
                     /gene="nirD"
                     /locus_tag="ACHK3A_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO8738541.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            6759..7565
                     /gene="nirC"
                     /locus_tag="ACHK3A_000399"
     CDS             6759..7565
                     /gene="nirC"
                     /locus_tag="ACHK3A_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO8738542.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSIRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            7584..8957
                     /gene="cysG"
                     /locus_tag="ACHK3A_000400"
     CDS             7584..8957
                     /gene="cysG"
                     /locus_tag="ACHK3A_000400"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO8738543.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGQVVGLRDK
                     LNWFSNH"
     gene            9208..9375
                     /gene="yhfL"
                     /locus_tag="ACHK3A_000401"
     CDS             9208..9375
                     /gene="yhfL"
                     /locus_tag="ACHK3A_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO8738544.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(9482..10567)
                     /gene="yhfS"
                     /locus_tag="ACHK3A_000402"
     CDS             complement(9482..10567)
                     /gene="yhfS"
                     /locus_tag="ACHK3A_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO8738545.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVSRIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HYAIRINPNRSGEETVLRILRESIASI"
     gene            complement(10579..11883)
                     /gene="yhfT"
                     /locus_tag="ACHK3A_000403"
     CDS             complement(10579..11883)
                     /gene="yhfT"
                     /locus_tag="ACHK3A_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO8738546.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(11895..12248)
                     /gene="yhfU"
                     /locus_tag="ACHK3A_000404"
     CDS             complement(11895..12248)
                     /gene="yhfU"
                     /locus_tag="ACHK3A_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO8738547.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(12259..13137)
                     /gene="php"
                     /locus_tag="ACHK3A_000405"
     CDS             complement(12259..13137)
                     /gene="php"
                     /locus_tag="ACHK3A_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO8738548.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNIVKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(13134..14360)
                     /gene="yhfW"
                     /locus_tag="ACHK3A_000406"
     CDS             complement(13134..14360)
                     /gene="yhfW"
                     /locus_tag="ACHK3A_000406"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO8738549.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPMLEKMGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALLNADWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITFNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMVATQLGVLTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(14360..15523)
                     /gene="yhfX"
                     /locus_tag="ACHK3A_000407"
     CDS             complement(14360..15523)
                     /gene="yhfX"
                     /locus_tag="ACHK3A_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO8738550.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPLAHQ
                     GHLVQIPCHQVSDAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVADIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(15607..15969)
                     /gene="yhfY"
                     /locus_tag="ACHK3A_000408"
     CDS             complement(15607..15969)
                     /gene="yhfY"
                     /locus_tag="ACHK3A_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO8738551.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGMM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVKPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(15986..16891)
                     /gene="yhfZ"
                     /locus_tag="ACHK3A_000409"
     CDS             complement(15986..16891)
                     /gene="yhfZ"
                     /locus_tag="ACHK3A_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO8738552.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVNRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKQALLAHQQRVVSGEQEPSY"
     gene            complement(17181..18185)
                     /gene="trpS"
                     /locus_tag="ACHK3A_000410"
     CDS             complement(17181..18185)
                     /gene="trpS"
                     /locus_tag="ACHK3A_000410"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO8738553.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(18178..18936)
                     /gene="gph"
                     /locus_tag="ACHK3A_000411"
     CDS             complement(18178..18936)
                     /gene="gph"
                     /locus_tag="ACHK3A_000411"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO8738554.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(18929..19606)
                     /gene="rpe"
                     /locus_tag="ACHK3A_000412"
     CDS             complement(18929..19606)
                     /gene="rpe"
                     /locus_tag="ACHK3A_000412"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO8738555.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(19624..20460)
                     /gene="dam"
                     /locus_tag="ACHK3A_000413"
     CDS             complement(19624..20460)
                     /gene="dam"
                     /locus_tag="ACHK3A_000413"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO8738556.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(20567..21853)
                     /gene="damX"
                     /locus_tag="ACHK3A_000414"
     CDS             complement(20567..21853)
                     /gene="damX"
                     /locus_tag="ACHK3A_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO8738557.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(21945..23033)
                     /gene="aroB"
                     /locus_tag="ACHK3A_000415"
     CDS             complement(21945..23033)
                     /gene="aroB"
                     /locus_tag="ACHK3A_000415"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO8738558.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(23090..23611)
                     /gene="aroK"
                     /locus_tag="ACHK3A_000416"
     CDS             complement(23090..23611)
                     /gene="aroK"
                     /locus_tag="ACHK3A_000416"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO8738559.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(24012..25250)
                     /gene="hofQ"
                     /locus_tag="ACHK3A_000417"
     CDS             complement(24012..25250)
                     /gene="hofQ"
                     /locus_tag="ACHK3A_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO8738560.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(25162..25566)
                     /gene="hofP"
                     /locus_tag="ACHK3A_000418"
     CDS             complement(25162..25566)
                     /gene="hofP"
                     /locus_tag="ACHK3A_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO8738561.1"
                     /translation="MRVKRWLLAGITLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            complement(25556..25996)
                     /gene="hofO"
                     /locus_tag="ACHK3A_000419"
     CDS             complement(25556..25996)
                     /gene="hofO"
                     /locus_tag="ACHK3A_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO8738562.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(25980..26519)
                     /gene="hofN"
                     /locus_tag="ACHK3A_000420"
     CDS             complement(25980..26519)
                     /gene="hofN"
                     /locus_tag="ACHK3A_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO8738563.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQLQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(26519..27288)
                     /gene="hofM"
                     /locus_tag="ACHK3A_000421"
                     /pseudo
     CDS             complement(26519..27288)
                     /gene="hofM"
                     /locus_tag="ACHK3A_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
     gene            27408..29960
                     /gene="mrcA"
                     /locus_tag="ACHK3A_000422"
     CDS             27408..29960
                     /gene="mrcA"
                     /locus_tag="ACHK3A_000422"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO8738564.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(30128..30688)
                     /gene="nudE"
                     /locus_tag="ACHK3A_000423"
     CDS             complement(30128..30688)
                     /gene="nudE"
                     /locus_tag="ACHK3A_000423"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO8738565.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            31008..33143
                     /gene="igaA"
                     /locus_tag="ACHK3A_000424"
     CDS             31008..33143
                     /gene="igaA"
                     /locus_tag="ACHK3A_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO8738566.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAHDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            33208..33876
                     /gene="yrfG"
                     /locus_tag="ACHK3A_000425"
     CDS             33208..33876
                     /gene="yrfG"
                     /locus_tag="ACHK3A_000425"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO8738567.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            33887..34288
                     /gene="hslR"
                     /locus_tag="ACHK3A_000426"
     CDS             33887..34288
                     /gene="hslR"
                     /locus_tag="ACHK3A_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO8738568.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            34313..35191
                     /gene="hslO"
                     /locus_tag="ACHK3A_000427"
     CDS             34313..35191
                     /gene="hslO"
                     /locus_tag="ACHK3A_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO8738569.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(35329..37053)
                     /gene="yhgE"
                     /locus_tag="ACHK3A_000428"
     CDS             complement(35329..37053)
                     /gene="yhgE"
                     /locus_tag="ACHK3A_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO8738570.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAEYMKDPKRRRGLLMAFDGVQRLQQQISEKA
                     LADNVFIASGSGKPDTIFWSAVMKNRYNAMICIEKDACVLVEQDLNSDGRAERILFAF
                     NDERYIIYGIDPAGKEWKEITMNSLPAVITKEKLLMAAEDGKLGTKPKVWRDLTVDGE
                     RLDLNLNE"
     gene            37432..39054
                     /gene="pckA"
                     /locus_tag="ACHK3A_000429"
     CDS             37432..39054
                     /gene="pckA"
                     /locus_tag="ACHK3A_000429"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO8738571.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTRILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            39169..39486
                     /locus_tag="ACHK3A_000430"
     CDS             39169..39486
                     /locus_tag="ACHK3A_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8738572.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            39545..39841
                     /gene="nadS"
                     /locus_tag="ACHK3A_000431"
     CDS             39545..39841
                     /gene="nadS"
                     /locus_tag="ACHK3A_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO8738573.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(39873..41225)
                     /gene="envZ"
                     /locus_tag="ACHK3A_000432"
     CDS             complement(39873..41225)
                     /gene="envZ"
                     /locus_tag="ACHK3A_000432"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO8738574.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGSSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(41222..41941)
                     /gene="ompR"
                     /locus_tag="ACHK3A_000433"
     CDS             complement(41222..41941)
                     /gene="ompR"
                     /locus_tag="ACHK3A_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO8738575.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            42169..42645
                     /gene="greB"
                     /locus_tag="ACHK3A_000434"
     CDS             42169..42645
                     /gene="greB"
                     /locus_tag="ACHK3A_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO8738576.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            42742..45063
                     /gene="yhgF"
                     /locus_tag="ACHK3A_000435"
     CDS             42742..45063
                     /gene="yhgF"
                     /locus_tag="ACHK3A_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO8738577.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            45501..45728
                     /gene="feoA"
                     /locus_tag="ACHK3A_000436"
     CDS             45501..45728
                     /gene="feoA"
                     /locus_tag="ACHK3A_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO8738578.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            45745..48066
                     /gene="feoB"
                     /locus_tag="ACHK3A_000437"
     CDS             45745..48066
                     /gene="feoB"
                     /locus_tag="ACHK3A_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO8738579.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            48066..48302
                     /gene="feoC"
                     /locus_tag="ACHK3A_000438"
     CDS             48066..48302
                     /gene="feoC"
                     /locus_tag="ACHK3A_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO8738580.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGNCKSCPEGKACLREWWALR"
     gene            48505..49386
                     /gene="rpnA"
                     /locus_tag="ACHK3A_000439"
     CDS             48505..49386
                     /gene="rpnA"
                     /locus_tag="ACHK3A_000439"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO8738581.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAEHSPKHKESLMTIAERLRQEGFQKGIRAIAKTMLDAGVPLEDV
                     LRFTSLTVEDLAEINHQ"
     gene            49458..49577
                     /locus_tag="ACHK3A_000440"
                     /pseudo
     CDS             49458..49577
                     /locus_tag="ACHK3A_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49606..50376)
                     /gene="bioH"
                     /locus_tag="ACHK3A_000441"
     CDS             complement(49606..50376)
                     /gene="bioH"
                     /locus_tag="ACHK3A_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO8738582.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKMVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            50414..51097
                     /gene="gntX"
                     /locus_tag="ACHK3A_000442"
     CDS             50414..51097
                     /gene="gntX"
                     /locus_tag="ACHK3A_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO8738583.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALARLLLLEVL
                     HARRTTGLQLPDRIISVPLWQQRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            51156..51731
                     /gene="nfuA"
                     /locus_tag="ACHK3A_000443"
     CDS             51156..51731
                     /gene="nfuA"
                     /locus_tag="ACHK3A_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO8738584.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            52092..53408
                     /gene="gntT"
                     /locus_tag="ACHK3A_000444"
     CDS             52092..53408
                     /gene="gntT"
                     /locus_tag="ACHK3A_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO8738585.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(53453..55537)
                     /gene="malQ"
                     /locus_tag="ACHK3A_000445"
     CDS             complement(53453..55537)
                     /gene="malQ"
                     /locus_tag="ACHK3A_000445"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO8738586.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTFTQEDQRVHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQTRDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QVAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGSETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(55547..57940)
                     /gene="malP"
                     /locus_tag="ACHK3A_000446"
     CDS             complement(55547..57940)
                     /gene="malP"
                     /locus_tag="ACHK3A_000446"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO8738587.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELAEYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWTNDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPQVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            58562..61267
                     /gene="malT"
                     /locus_tag="ACHK3A_000447"
     CDS             58562..61267
                     /gene="malT"
                     /locus_tag="ACHK3A_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO8738588.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGDWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWHNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(61310..62326)
                     /gene="rtcA"
                     /locus_tag="ACHK3A_000448"
     CDS             complement(61310..62326)
                     /gene="rtcA"
                     /locus_tag="ACHK3A_000448"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO8738589.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSSTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(62330..63556)
                     /gene="rtcB"
                     /locus_tag="ACHK3A_000449"
     CDS             complement(62330..63556)
                     /gene="rtcB"
                     /locus_tag="ACHK3A_000449"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO8738590.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            63745..65343
                     /gene="rtcR"
                     /locus_tag="ACHK3A_000450"
     CDS             63745..65343
                     /gene="rtcR"
                     /locus_tag="ACHK3A_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO8738591.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRL
                     ELLHDARSRSLFETLKCDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PDKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEMNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLSQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITREVVEDEINRLRYNWQES
                     RPSAITALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(65325..66083)
                     /locus_tag="ACHK3A_000451"
     CDS             complement(65325..66083)
                     /locus_tag="ACHK3A_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO8738592.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(66100..66930)
                     /gene="glpG"
                     /locus_tag="ACHK3A_000452"
     CDS             complement(66100..66930)
                     /gene="glpG"
                     /locus_tag="ACHK3A_000452"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO8738593.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(66975..67301)
                     /gene="glpE"
                     /locus_tag="ACHK3A_000453"
     CDS             complement(66975..67301)
                     /gene="glpE"
                     /locus_tag="ACHK3A_000453"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO8738594.1"
                     /translation="MEQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWRRQFPA
                     EVAYGA"
     gene            67491..68996
                     /gene="glpD"
                     /locus_tag="ACHK3A_000454"
     CDS             67491..68996
                     /gene="glpD"
                     /locus_tag="ACHK3A_000454"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO8738595.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(69050..69655)
                     /locus_tag="ACHK3A_000455"
     CDS             complement(69050..69655)
                     /locus_tag="ACHK3A_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738596.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMGGIGWQFSRRTPPELKNRLHYWLVFSPAY
                     FWLILSNFILYITPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSQGQKWVEWGTCLSLAILAQGPLFNYLQ"
     gene            complement(69658..70434)
                     /locus_tag="ACHK3A_000456"
     CDS             complement(69658..70434)
                     /locus_tag="ACHK3A_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738597.1"
                     /translation="MQPSSAMQALIEQVYHIFRRYPVPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDERAILQRFACQYMADWVSGDEAVELQYMLEMFSRADIALSPLLDAIIS
                     VPGYQPTVSMACLLWHNREENIQNSRFEQDDDDKAITAQINAWAFNNQFILKERARQA
                     IENLLNQPEQGTHYQVWEDDWMIDECLCAMYDASSESPGK"
     gene            complement(70437..71147)
                     /locus_tag="ACHK3A_000457"
     CDS             complement(70437..71147)
                     /locus_tag="ACHK3A_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738598.1"
                     /translation="MELVDTLFASLSGTDPFTGVDITIANCKSTYWDEGIIQQLINQV
                     LDEGEKFAGTVGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATIGRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDFTVHFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKEAW
                     KFHTDNSLRGFRDKQAELKLYARHYSVENCTEESCRDCNYCIRPSFSLSRSSIIRLNA
                     ANARFVYQPFTRDQRKRG"
     gene            complement(71321..72442)
                     /locus_tag="ACHK3A_000458"
     CDS             complement(71321..72442)
                     /locus_tag="ACHK3A_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8738599.1"
                     /translation="MITYAGYKVKIIFIIFLFLGYHVVAYAEMGRDREFCYPGTPGNN
                     TTPATFFYDFGTVIISDVNNNSAGTVLPSQNWKIGRYKAYCNSLDDYEVYFSGISGID
                     PSGASGNPQGAEIFIPVTHEISISTHIKLYNQNGTMTDKTVPFENYSTNYPADRSKAD
                     YWSSGTEGYIKIRLDKKIISDVSLNNVLLVSLYASQRQTEHGPVPLFNAWISNLDIQV
                     PQGCTINEGTSFTVNMPDVWASELSRAGAGAKPAGVTPVATTIPINCTNKDTDAVMTL
                     VFDGNISATRDTNGKQSIIQAQDNPDVGIMIMDSQQNSVDLNALATSVGVPFRLVENQ
                     AASAQTADVTFLALPVSTTGKVPAAGRYNALAVLRVEYQ"
     gene            complement(72457..73236)
                     /locus_tag="ACHK3A_000459"
     CDS             complement(72457..73236)
                     /locus_tag="ACHK3A_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8738600.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSFSGTVFIATPPLFVLKPDKNTK
                     IRINRVGGSLPADRESLFILNVAALPSLENSHPTKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNISYQKLRWARKNEIVTVYNPGPRYVTLYNLHVDGKVMDGGMIAPFSHRQQSWCK
                     AQGICEITWQTLDIYNNVLPVWKVNMNPLQMDVSGSQFKTD"
     gene            complement(73202..73774)
                     /locus_tag="ACHK3A_000460"
     CDS             complement(73202..73774)
                     /locus_tag="ACHK3A_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8738601.1"
                     /translation="MPGKQMLCCILISIISEGDMKIFISLFLFIISTNSFADDITHAG
                     VVRIEGLITEKTCIISDESKNFTVNMPDVPSSSVRSAGDVTEKVYFSITLTRCGSDVG
                     NAYIKFTGNTVSEDASLYKLEDGSVDGLALTIFDKNKGSISNDVKSIGFSLTSSVDNI
                     LHFFAAYKALKNNVQPGDANASVSFIVTYD"
     gene            complement(73725..>74208)
                     /locus_tag="ACHK3A_000461"
     CDS             complement(73725..>74208)
                     /locus_tag="ACHK3A_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8738602.1"
                     /translation="DSVVVTFTGILLAKSCDITTGSKDQSVKMGTYDANEFLNVGDVS
                     PSQTFTINVQGCPTAKSTVYSSGVSANVRFTGDADAINSALLRLSAGADSATGLGIEI
                     LDNNDVAIAINGESGFRDLVLNENGDANLTFKLRYKSTQENVHAGQANALLYFDIDYQ
                     "
BASE COUNT        18107 a        19924 c        18423 g        17754 t
ORIGIN      
        1 cactgaatgg caatcagaaa tcagcgatgc agaaaattag ccagcagttg atgtccttgt
       61 tcgctgagaa tactttctgg atggaattgc acaccttcca gatcccactg gcgatggcga
      121 atccccataa tctcgcgggt ttcgctccag gccgtcactt caaagcactc cggtaatgag
      181 tcaggttcca ccaccagcga atggtagcgt gtcacggtga gtggatttgc cagcccctta
      241 aatacgccca caccattatg tgtaatcggc gaggttttgc cgtgcatgac ctttgcggcg
      301 cgcacaactt tgccgccaaa tgcctgcgcc attgcctgat gaccgaggca gacgccaaga
      361 atcggcaagc gtccggcata gtgacgaata acgtcaaggg agatcccggc ttcatctggc
      421 gtacaggggc cgggtgagat gacaattttt tgtggtttaa gggcgtcgat atccgccaac
      481 gtcaacgcat cgttgcgctt aaccaacaca tccgccccca gttcacaaaa gtactggtag
      541 aggttccagg taaaagaatc gtagttatca ataagcagga tcatggcggc tccggtacga
      601 aagaaccgcg ctattttact cagattcccc ggcttcgctt accactttgc taaatatcat
      661 ctgcagcgcg gtgagatccc ccattgcccc attctgattt gcctgattcc aggcctcttt
      721 ttcaataccc tgccagctca gttgatatcc ggcatgaatc gccagttgct cgaagaagat
      781 ccgctgtgcc aaaccacttc ccacccggaa gggatgcagc acgttgattt cacaatagta
      841 atgcgccagc cgctcgacga acttcgcttt ctccaggcca accagataac cttcttcctc
      901 cagatcctgc atcagggcat tgccctcttt ttcgatataa gcaaagtggc agaacggcgt
      961 atcgccctga taaatatcga cttcgcgcag ttgccccgcc cagtcgaaaa tatcctgata
     1021 caactggcga tgaagagtac gcaaatgcgg taaaccccga accaacgggc caagttcaat
     1081 ggtcgcagca cgcagcgccg tcatttcgta agcggcctgt tccagccgct gctgctggcg
     1141 gatgttcagc cggttacgca tgatatcaag gcctggataa agatacggat cgcgcccttc
     1201 gccgaattta tcgctcatag tgcctcctca gcgcttcaag acgcgctaaa gcctctgcgg
     1261 cagtaagagt gactaaaggc atctcgacgc cttcaagacg gcgactggcc tggaaattac
     1321 gattacgctg aagctcccag agacgggact tttgcttatc ggtgagtttc ttcacttgat
     1381 gcctccctga acgtgtccat ttgccacaag tataagcggc aaatccgggt tacgccggga
     1441 gagcagaagc gcgggcaaga ttgcccgcgc gagaaatcat tacggcagga ctttagcgga
     1501 aaggataact accggttttg acggcacatt ctggtacgga ccaacgtcat gagtcggcac
     1561 ctgggaaatc ttatcggcaa cgtccatgcc tttcaccact ttaccaaata ccgcgtaacc
     1621 gaaatcacgc tgaccgtggt caaggaaggc gttatcggca acgttgataa agaactggct
     1681 ggtggcgctg tctttgtcag cggtacgcgc catcgcgatg gtgccacgcg tgttgcgcag
     1741 gccgttatca gcttcatttt tgattggcgg gtttggtttt ttctgctgca tctgctcggt
     1801 gaaaccgccg ccctgaatca taaagccagg aatgacgcgg tgaaaggtcg tgttgttata
     1861 aaaaccactg ttcacataat cgacaaagtt ttgcacagac actggcgctt tttgtttatc
     1921 cagctccagt tcgatgttac cagctgaggt tgtcaacaat acgtgcgggt cccctttcgc
     1981 tgccattgct gcgggagaaa gagcagaaag agcgaaaaca gcagccatcg ccgccagggt
     2041 cgatttgaac atgagatttc cttaacaaac agagcagaaa aaaagcaagt gaagagattc
     2101 taaatagcct tcaagaacaa ggccatccct ttacctaatt ttacgtatct gaaatatatg
     2161 taacctgagg ataacttttt attccgtgat caaaatcacc tcttaaaatg caatttagca
     2221 accgattgca ataaaacatt taatcagatc acaaaatcgc ctaaaatcgc ccgctcgcgg
     2281 catagcccgc tatgccattt tatttttgaa cgcaggccaa tcatgactaa cagcaatcgc
     2341 atcaagctca catggattag ctttctttcc tacgcactga ccggtgcgtt ggttattgtc
     2401 accgggatgg tgatgggaaa tatcgccgat tatttcaatc tgcctgtttc cagcatgagt
     2461 aacaccttca ccttcctcaa tgccggcatt ttaatctcta tcttcctcaa cgcctggctg
     2521 atggaaatcg tcccgttgaa aacgcagtta cgttttggct ttctcctgat ggtgctggcg
     2581 gttgccggtt tgatgttcag ccacagcctg gcgctgttct cggcggcgat gttcattctc
     2641 ggggtggtca gcggcatcac catgtcgatt ggtacattcc tggtaacaca aatgtatgaa
     2701 ggacgtcagc gcggttcacg cctgttattt accgactcct tcttcagtat ggccgggatg
     2761 attttcccaa tgatcgccgc gtttctgctg gcacgtagca ttgagtggta ctgggtttat
     2821 gcctgcatcg ggctggtgta tgttgctatt tttattctga ccttcggctg tgaattcccg
     2881 gcactgggca aacatgcgcc aaaaacagat gctccggtag agaaagaaaa gtgggggatc
     2941 ggcgtactgt ttctctctgt tgcggcgctg tgctacatcc tcggtcagtt aggttttatc
     3001 tcctgggtgc cggaatatgc caaaggcctg ggcatgagcc tgaacgacgc aggcacgctg
     3061 gtaagtaact tctggatgtc gtacatggtc ggcatgtggg cgttcagctt tattcttcgc
     3121 ttctttgatt tgcaacgtat cctgatcgta ctggctggtc tggctgcgat tctgatgtac
     3181 gtctttaaca ccggaacacc ggcatatatg gcgtggtcaa ttctcgccct gggcttcttc
     3241 tccagcgcga tctataccac catcatcacc ctgggttcac agcagaccaa agtaccgtcg
     3301 ccaaaactgg ttaactttgt cctgacctgc gggaccatcg gtactatgtt gacctttgtg
     3361 gttaccggcc cgattgttga acatagtggt ccgcaggcgg cgctgctgac ggcaaacggt
     3421 ctgtacgctg tcgtctttgt gatgtgcttc ctgttaggtt tcgtcagccg tcaccgtcag
     3481 cataacaccc tgacctctca ttaattgttc atgccgggcg gcactatcgt cgtccggcct
     3541 tttcctctct tactctgcta cgtacatcta tttctataaa tccgttcaat ttgtctgttt
     3601 tttgcacaaa catgaaatgt cagacaattc cgtgacttaa gaaaatttat acaaatcagc
     3661 aatatacccc ttaaggggta tataaaggtg aatttgattt acatcaataa gcggggttgc
     3721 tgaatcgtta aggtaggcgg taatagaaaa gaaatcgagg caaaaatgag caaagtcaga
     3781 ctcgcaatta tcggtaacgg tatggtcggc catcgcttta tcgaagatct tcttgataaa
     3841 tctgatgcgg ccaactttga tattaccgtt ttctgtgaag aaccgcgcat cgcttatgac
     3901 cgcgtacacc tctcgtctta cttctctcac cacaccgccg aagagctgtc gctggtgcgc
     3961 gaaggcttct acgagaaaca cggcatcaaa gttctggtcg gcgaacgcgc tatcaccatc
     4021 aaccgtcagg agaaggtgat tcactccagc gccggacgta ccgtttttta tgacaagctg
     4081 atcatggcga ccggttccta cccgtggatc ccgccaatta aaggttctga tactcaggac
     4141 tgctttgtct atcgcaccat tgaagacctc aacgccattg aatcctgcgc tcgtcgcagc
     4201 aagcgcggtg ccgttgttgg tggcggcctg ttaggtctgg aagctgctgg cgcgctgaaa
     4261 aacttaggca ttgaaaccca cgttatcgaa tttgccccta tgctgatggc ggaacagctt
     4321 gatcagatgg gcggcgagca gctgcgtcgc aaaatcgaaa gtatgggcgt gcgcgttcac
     4381 accagcaaaa acactcttga gattgtgcag gaaggtgttg aagcgcgtaa aaccatgcgt
     4441 tttgccgacg gcagcgaact ggaagtcgac tttatcgtct tctctaccgg tatccgtccg
     4501 cgcgataagc tggcaaccca gtgtggtctg gacgttgctc cgcgtggggg tatcgtcata
     4561 aatgattcct gccagacttc cgatccggat atctacgcca tcggtgaatg cgcaagctgg
     4621 aacaaccgtg tatttggtct ggtagcacca ggctacaaaa tggcgcaggt cgccgttgac
     4681 catattcttg gtagcgaaaa cgcctttgaa ggtgcagacc ttagcgccaa gctgaaactg
     4741 ctgggcgtag acgtaggcgg tattggtgat gcgcacggtc gcacacctgg cgcacgtagc
     4801 tacgtttacc tcgacgaaag caaagagatc tacaaacgcc ttattgtcag cgaagacaac
     4861 aaaaccctgc tcggtgcggt actggtgggc gataccagcg actacggcaa cctgctgcaa
     4921 ctggtgctga atgctatcga gctgccggaa aacccggatt ctctgattct gccggcgcac
     4981 tcgggtagcg gtaagccgtc tatcggtgtt gataaactgc cggacagcgc gcaaatctgc
     5041 tcctgcttcg acgtcaccaa aggtgatctg attgctgcca tcaacaaagg ctgccacacc
     5101 gttgcggcgc tgaaagctga aaccaaagcg ggtactggct gcggtggctg tatcccgctg
     5161 gtcactcagg tactgaacgc ggaactggcg aaacagggca tcgaagttaa caacaacctg
     5221 tgcgaacact ttgcttattc gcgtcaggaa ctgttccatt tgatccgcgt tgaaggcatt
     5281 aaaaccttcg aagaactgct ggcgaaacac ggcaaaggct acggttgtga agtttgtaaa
     5341 ccgaccgtcg gttcgctgct ggcctcctgc tggaacgaat acattctgaa accggaacat
     5401 actccgctgc aggattctaa cgataacttc ctcgctaaca tccagaaaga cggcacctac
     5461 tcggtgatcc cgcgttctcc gggcggtgaa atcaccccgg aagggctgat ggcagtaggt
     5521 cgtatcgcgc gtgaatttaa tctctacacc aaaatcaccg gctcccagcg cctggcgatg
     5581 tttggcgcac agaaagacga tctgccggag atctggcgtc agctgattga agccggcttc
     5641 gaaaccggtc atgcctatgc gaaagcactg cgtatggcga aaacctgcgt gggtagcacc
     5701 tggtgccgct acggcgtggg cgacagcgtc ggcctcggcg tggaactgga aaaccgctac
     5761 aaaggtatcc ggactccgca caaaatgaag ttcggtgtct ccggctgtac ccgtgaatgt
     5821 tcagaagctc agggtaaaga cgtgggtatt atcgccaccg aaaaaggctg gaacctgtat
     5881 gtttgcggta acggcggcat gaaaccgcgt catgcgaatc tgttggcagc ggatatcgat
     5941 cgcgaaacgc tgatcaaata tctcgaccgc ttcatgatgt tctacatccg taccgccgac
     6001 aaactgacgc gtaccgcgcc gtggttagaa aacctcgaag gtggcatcga ttacctgaaa
     6061 gcggtgatca ttgacgacaa actggggctg aacgcacatc tggaagaaga gatggcgcgc
     6121 ctgcgtgaag cggtagtgtg tgagtggact gaaacggtca ataccccgtc tgcgcagact
     6181 cgcttcaaac acttcatcaa cagcgataag cgtgacccga acgtgcagat ggtgccagag
     6241 cgcgaacagc accgtccggc aacgccgtat gaacgtatcc cggtaactct ggtggaggac
     6301 aacgcatgag ccagtggaaa gacatctgca aaatcgatga catcctgcct gaaaccggcg
     6361 tctgcgcgct gttaggtgac gaacaagtag caattttccg cccgtatcac agcgatcagg
     6421 tgtttgcgat cagcaacatc gatccgttct tcgagtccag cgtgctgtca cgcgggctga
     6481 ttgcggaaca ccagggcgag ctgtgggtcg ccagcccgct gaaaaaacag cgttttcgct
     6541 taagtgatgg cttgtgcatg gaagacgaac agttttccgt caaacattac gaagcacgag
     6601 tgaaagacgg cgtggtgcag ctgcgcggtt aatgttttaa cgggaggcgc aatgcctccc
     6661 ctttttgcat ggtcctgtaa tgaccttcgg tatattgcag gacatttttt aaactttttg
     6721 ttttattttt ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata
     6781 agtgtgcggc taacgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt
     6841 gggtcagctc cgccatggcg ggcgcgtatg tgggtcttgg gatcatcctg attttcacgc
     6901 tcggtaattt gctcgatccg tccatacgtc ctctggtgat gggcgcgacc tttggtatcg
     6961 ccttaacgct ggtgattatc gccggttctg aactgttcac cggacacacc atgttcctca
     7021 cctttggggt gaaagcgggc accatcagcc acgggcaaat gtgggcaatc ctgccgcaaa
     7081 cctggctggg taatctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg
     7141 gtagcctgct gccggtagat accagcatcg ttcactccgt cgcgctggca aaaaccactg
     7201 caccggcaat ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta
     7261 tctggatggc gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc
     7321 tggcatttat cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct
     7381 cctggttcgg caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt
     7441 gggtgacgct gggtaatact ttatcaggtg ccgtattcat gggattgggt tattggtatg
     7501 ctacgccgaa agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acagctgccg
     7561 gttaattact aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc
     7621 gcgactgtct gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttactgttag
     7681 acgcaggcgc tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg
     7741 cagatgcagg catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct
     7801 gctggctggc gattgctgcg acggatgatg acgcgcttaa ccagcgcgtc agtgaagccg
     7861 ctgaagctcg tcgcatcttc tgtaacgtgg tcgatgcgcc gaaagccgcc agctttatta
     7921 tgccgtcaat tattgaccgc tcaccgctaa tggtcgcggt ctcctctggc ggcacctctc
     7981 cggttctggc gcgcctgttg cgcgaaaaac tcgaatcact gctgccgctg catctgggcc
     8041 aggtagcgaa atacgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatgg
     8101 gtgagcgtcg ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg
     8161 caaacaacga tcagaaagcc attactgaaa ccaccgaaca gttaatcaac gaaccgctcg
     8221 accatcgcgg tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacac
     8281 tgaaaggtct gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg
     8341 acgatattat gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg
     8401 gataccactg cgtaccgcag gaagagataa accagatcct gctacgggaa gcgcaaaaag
     8461 gcaaacgcgt ggtgcggctg aaaggcggcg atccgtttat ttttggccgt ggtggcgaag
     8521 agctggaaac actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag
     8581 cttctggttg ctctgcctat tcgggtattc cgctcacgca tcgcgattat gcccagagcg
     8641 tacgcttaat taccggacac ttaaaaaccg gtggcgaact ggactgggaa aacctggcgg
     8701 cagaaaaaca gacgctggtg ttctatatgg ggttgaatca ggccgcgact attcagcaaa
     8761 agctgattga acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacag
     8821 cagtcacgca gcgcgtgatt gacggtacgc tcacacaact gggcgaactg gcgcagcaaa
     8881 tgaacagtcc atcgctaatt attattggtc aggttgttgg cctgcgcgat aaattgaact
     8941 ggttctccaa ccattaattt aaccaaggcc agagaattct ggcctttttc gtttacaact
     9001 ctctttatat attcagcaga taaaaacact ccccttaata aatattatct gctaatgggt
     9061 tatattcaac gttacagcga cttacaaaat taagtcattc actcactgac gccgattaaa
     9121 tattcctgta tcgttcttaa tgagcagtct gcttatattc atataataaa tgaatattaa
     9181 tcaacatata atacataggg aatgtaaatg aacaaattta ttaaagttgc actggtaggt
     9241 gcggtactgg ctacgttaac tgcatgtact ggtcatattg aaaaccgtga taagaactgc
     9301 tcttacgact acctgctgca cccggcaatt tctatttcta aaatcattgg cggttgcggt
     9361 cctactgcac agtaagtctc tgactgaaag cggctaacac cggtttaatt gcctgacgcc
     9421 ctcggggtta cccgagggcg tatttatttc tgcattcttt tcgaaaacat catgcacaag
     9481 attaaatact ggcaatactc tcacgcaaaa tccgcagcac cgtctcttca ccgctgcgat
     9541 ttgggttaat acgaatcgcg taatgttcga gttgcggatt cgcctggcga aacgttccgg
     9601 aaagtcgata aaaaagtggc ggaatttcat atttcgactc tgcaccaacc gggtaaggca
     9661 aggcaccgcg cttttgcgcc tcttccagta ctctggcggc aatcggctga tgaaactcca
     9721 caatcaacac cttcgactgc gcattggcaa tcaccgcgct tttcacttcc gccactgcgc
     9781 caccgttaag taaagccagc aaccgttcag atacccccgc ctgcaccgcg tgcatcactg
     9841 gcgcgagcac cagaccacgc aacacctcta atgcctgcgc cccctgaatc tgactgccgc
     9901 cggaatagag cgtggcgcga atccggctaa taacatcggc atcgcctacc accgcaccaa
     9961 cgccctctgg cccaaatagc ttgaagcagg aaaatgtcga cacattcgcc ccgcattcgc
    10021 agccaattcg cgacaccttc atcaccgcat agttgtcatc ggttaacgct ggaacacctg
    10081 ccgcgcgcaa cgttgccagc acgtctgcca gcacatagcg gtcctgcggc tgctggcgcg
    10141 tatgctgcac cagcgcggca tcaggttgtt gctcgtcaac aacctgcttc agtgccgaca
    10201 gatcattgaa atcagccgta atgagcgtta gccccatctg ctcaataatg acgcgcgtcg
    10261 ttggataaac cggcgcatca tgcaccagaa gacgttgccc cggtttgagc aaagccgcca
    10321 gtgccgcacg aatcgcgcca gtacccgcgc cctgtaccag tgccgccgcg ggtgcatgaa
    10381 acgcgtcagc cagcacctgt tccacacgct gggtaatgcg gggctgattt agccccggcg
    10441 ttaagcccaa atcaccgcag gtaagaaact cgctgccagg aaaatgacgg caaatcgtgt
    10501 ccaccagcgc aaactgcttt tgctgcgcct caataagcgt caggctttgc agaggaaacg
    10561 tcttcatagg tgccttcctt aagccggaac gaacaggcca agccagtaaa gaacattcag
    10621 caggatgccg gtgatcatta ctgctacgac cggtgccgcc attttctgta ccggacggcc
    10681 cagggattcg ttgaggaagt aaatggcaac cgcgatagag aatccggtat agcccgccat
    10741 tttgattgct gcaaagatcg aaccgaccag caacgccact tccatcagca tattcatggc
    10801 gttacggatg ttatccgacg cattacgtac cgacgggtag cgccccaacc atttgccgat
    10861 cgaacgaagc agcaagactt ccgccgaaat aaccactgcg cctaataccg ctgcaaccat
    10921 cggattcggc gagagatagc ccaccgcata aacaaaggta aagcccgcaa ctgcatacac
    10981 accagtggct aacgcggtgg tggcaatcaa cggtacaaaa cccagcccgc gcataaattc
    11041 cgccagagct gcctggttga ttagtgtttg cgattgttcc ggcgttacgc ctgcggaata
    11101 tgctttctcc agtgtgaaga tcgacacttc actgccagca aaaatcttca tgctggcaac
    11161 ggcggcaatc agcgccccga caatagcgat atacggtaag tttttgataa tccgtgacgt
    11221 gcgttcttca aacaccgaaa gcccgctggc atcaatgtca ttttcatcac gatgacgcag
    11281 gtcgtgagta atcgcgatcc ccagcagcat caccatgcca ataaagattt cgatggattc
    11341 cggattgaga tgcgggaaat agcgcactac taccacacgg gtcatcagta ccaccacggc
    11401 ggcgatcaga ctttgcttcc agccaaactg gtagaaaatc gccaccaacg ggaacagtgc
    11461 aaaagctgaa accaccggcg agcttaattc ccccaggcta cctaatacat ccaccggtag
    11521 cgcggtcagc agctggttta ccggtaacag gcaagtaagg atcaacacgc cccagatagc
    11581 gccaagacca aacgccatca ggctgtttat cgccagtacg ccgaggatat cggtaggaag
    11641 aaagagtaac catgcgttga gcagtccggt tttcagggta aaagagatcc ccaccgaagc
    11701 cacaaaacca atgctcagac caaaagcgat actccccgcc tcgcgacggt tcatatagcc
    11761 ttcaatcagt tgcggcagga tcgggcggat accgtcatga aaaacggccg ccgagcgatg
    11821 cgccagaagc gatgtcatac ccgtcaggca cgccaccacg ataatctgaa tatacagatc
    11881 catagtcgtg ccttttattt cagatggtta atcagcatcg gaatcgcgtg ttcgacgtgc
    11941 tcaacggaaa gcccaaacgc cactttccct tcggcgatca ttttggcgat atgttcgtct
    12001 ttcgctttaa tgcctggttt ggcaatggtg caacttttgt tatagccaat caccgcgatg
    12061 gcaatcgaca atgccgcgcc cgcaccggta ttacacgcgc cgatgtagta atccagttgc
    12121 ccggatttga ctttcattgc cgcttccatg tcgttgtgaa tgaaaacttc aaaacagcca
    12181 ggagccgtcg cttcaatggt ttttttaatc tgctcacgct gtaagcctgc aacaccaatc
    12241 tttttcatga gtctgtcctt attggaaaaa ttgagaggga gtttcacgta acatcacatc
    12301 cacatcggcc tgactgaatc ctgactggcg caattgcgga ataaaggtgg tcaataaata
    12361 gtcatagcca taaccaccgt tggcttttaa atgggagcgg cgcgtaatat ccatcgacag
    12421 catgacgcgg ttaagcaaac ctcggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc
    12481 gtccgggtag taactgttct taccgatggt gtcgaactgc acatacgcgc cgagatcgat
    12541 catcttcaca atgttgtcga ggttgtcttt cagatcgcag tgaccaacgg tgacgcgcga
    12601 aagatcaact ccgtgggctt gcagtagcgc cagttgttcc agccccatgg tgctaaaaga
    12661 ggtatgcgtg gagatcgggc gtccggtctg gttatgcgcc agcgcggcgg caataaatac
    12721 cttctcttcc agcggcgtga tttttccttc gctggagcct atctccgcga tgatcccggc
    12781 tttcagctcc gtgccatcga taccctgttc aatttcatcg accatctctt gcgccagttc
    12841 ctgcacgctg cgggtcgcca catgttccgg gaaaaacgcg tcctggtaat aaccggtaca
    12901 ggccaccacg ttgatcccgg tttcgcgcat tacatcgagc ataaattgcg cattgcgccc
    12961 catgtaacgg ttggtcatct caatcacatt acgcacgccc tgggccatca ggtcgttcat
    13021 ctcctggcaa atgaacgcat actgatcaag gcggcagtcc acgttgtttt taaagccgga
    13081 gagatcaata tgcagatgct catgggcgag ggtgtagccc gtcggatcaa aactcatagg
    13141 gtgtctcctg caaaccggag ggaggaaaga aaagagcgac cattttgtgg cggtggcgcg
    13201 cggaaaaatt cacatacggt agcccccaca tcagaaagcg tggtgagcac accaagctgc
    13261 gtagcgacca tcccttgctg ataaaccagc actggcacca cttcgcgggt atggtggctg
    13321 tgaccaatgg tcggatcgtt accgtgatcc gccatcacga ccaggcaatc atctggctgc
    13381 atcgcctcaa taagccgggc aaggttacgg tcaacgacct gcaaacgttc cgcataacgt
    13441 gcgacgtctt ctgcatgacc agcgaggtcg gtttcctgga tattggtaca aataaacgcc
    13501 gtcggatggg tgttaaattc gttgaaggtg atatccataa tccgctggct atccaccagg
    13561 ttttgccagc tcacgccata aggattactg gcaatatccg ccactttccc caccagcacg
    13621 gttggtacgc ccacttcata cagtttttgt ggcacctgca ctttttcatc gacgccgtag
    13681 cccatatgca cgacctggaa gccgttgtca taagcgccag aacgcggcgc attgatgcca
    13741 ataaagcgcc cttctttgct ttctgcggca tcgaggatgc gctggctgtc ggttaacagg
    13801 ccaccaaatg taatgacccg accgacctgt acctgctcac gcacgatacg accaattttg
    13861 attgcgtcgt caaaagagat cacagagaga ttggcggtga tgttatagac ctgacctaaa
    13921 tccgcctcga gattatcgcc aatcgcaacc gcctgattga cccacagaaa ttgcagatca
    13981 tcgccacggc gctccacctg ccagtcagcg ttaagtaatg cttgctcaac acggtcaatc
    14041 acatcgcgaa agggcatccg tagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc
    14101 cccataaagg tatcgccacc ttcatgttgc agctctgcca cgccccaggt tgcggagtct
    14161 gacggctgca tatctccagg cgcataacct aatgcgttga ttagccccat tttctccagc
    14221 attggtagct gcaaatgcgg caactggctc aggatgtgac cacaggtatt tgccccggca
    14281 tcctgcggac gcaccaacgt gacatctttc attgccccta cgccaaagct atcaatcact
    14341 aacaccacaa atcgcgccat tacgccccca gagagtttcc aagactgtca taacgaccga
    14401 cgatttctgg ttcgccacgg tgaataccgg acagcaacac cacatcgcta cgggtgacaa
    14461 aaatctgcgt gcgaaaacag agcactactg cactgcttac cggaaactcg cctgccagcg
    14521 gcagggtgta gtcgatactg ctgtcatcca cagttttgag attggtttca gtaatctttt
    14581 gattttctgg cgtaaacacc agcgcatgtt gcgcgtgacc acgacggtag taaccgccgc
    14641 cgtagcagta gctgtcgcca cgaaaatgat gggagatttc acttaaccag agcatcgcga
    14701 tacgttcagg ctgatcgccc tgctggtttg ccggaatcgt ccccgtcagc gcatgaccgg
    14761 gttcggcatg agtcacaccg tattccgcca gcaatggcag cgaagtgcag ctggtcgcag
    14821 aaggtgcgtt cagttgctca atcgcaatac cagatttcgc cagttgatcc cgtgcctgta
    14881 tcagcgtgtg aagattcggt gtcggcaaaa ctttcccggc agcctcatcc caaagcaggc
    14941 aagggaaatg ggttagtccg gctaaatgca gccccggcag tttcttaata tcagcaacca
    15001 cctcgtgcag cgaatgctgt acaaaaccgc tctcctgccc cggataaaga aaatcatcgt
    15061 cgctataaac tttgagcagc acagactgca cacgtccggc cttcaccgct gctgcagaaa
    15121 cttcccgcgc tttatcgaga gtaaacacgg tgatgacgtc agttccctgt gcaacggcgt
    15181 cagaaacctg gtgacaaggg atttgcacca gatgcccctg atgcgccaac ggcagaccgg
    15241 cgcggcgcat gacccgcgcc tctttgtaat ccaccgccac aatgccgctg tagcccagtg
    15301 ccagaagttt ttccgccagc cacggattac ggccaaactg cttggtcatc agatacagtt
    15361 caatgccgta aagtcgcgcc gtctcaatca gccgcttacc gttttccagt atctgatcca
    15421 catcgatcac ccagctgtcc ggggcgatct tgccctgctg ccacaaactt agtgcggcgg
    15481 aaatcagcgc cgggttctgg cgtttcaatg cttctacaaa catcttgttg cctcgtttct
    15541 gttccttgaa ttaaatattc atttttctga atatttagat tatgcgacgt ggatttgtcg
    15601 ggagcttcaa acctcttcgt tagcagccat ccataatcca taaagattag ccagcaaata
    15661 gccttcttca cacggcttaa cttccagtgc gaattccttc aacaacacct gatgcaattg
    15721 cacaacggct tgccagtggc tggattgcgc cagctctgcc agcaattcgt catccagcgg
    15781 ctctatttct tcaccacggc gactgcgcat cagtgcgctc gccatatgtg tcatcgccat
    15841 cattccttgc tcgctgcgca ccggtagatg acactctgtt tccagtacgt tgacgacctg
    15901 catcatgcct ttgcagatgt ccttatcaat aacgcctgcc tcacaaagca ggttgagtct
    15961 ggtttccatt tcagatgcct ttcgtttaat aactcggttc ctgttcccca ctgaccactc
    16021 gttgttgatg ggcaagcagg gcctgtttat caacaactgc acgcagcaat tgttgcattg
    16081 ggtaatcatc gactcgcgtc aaaactacgg cttcggtggc ctgtaaaaat cgcggatcgt
    16141 ctgtgagcgg cgtcgcttct aatcccagca tggtcagttc gttttccgcc accacgttcc
    16201 agatcaccgc atcaacatcg cctttaacaa tgcgttgtaa actctcgtga taagagagat
    16261 cgactcgttc cacatcacta tcgccaaaaa agacatcggt catgattttc tgatccgccg
    16321 aacggttatc cagccccacg cgattcacgt ttgcggactc gcctttacgg caaatcaact
    16381 ggtgctcgcc aacgtaggtg tgcggcccca actccagcgc gaggcataac cctttttgcg
    16441 tgagataact ttccgccgcc agtcgggaaa caaccgccat gtcatacacg ccgttaagta
    16501 aacactccac gcgaatatcc gcgccgcgca tgtgcgcata gtaaaaagga ataccatcaa
    16561 actgagcttt caatccgctc gccaggcctt cgtacaaacg ggtatagggc aagggcatcg
    16621 cgcacaccac attgttgata tccacatgac tcagcaatgc tttgttatcc atttccacca
    16681 gataactgcc attacgccca cggcgttcta tccgtatcgc tccgcttgat tccagcgttt
    16741 tcaacgcggc ctgcgtcagg ccaacggatg aacggcattc agttgccagt tcatctatgg
    16801 ttttcagtcg attaccgcac ttttcaccca acaaataacg ggccagtgtc gtgatgacga
    16861 cgccttcttt tttgataaac gttcgacgca taataaattt tcagtaaatt gaatatttat
    16921 atcttcagga atttgaagat aaggcgcaac agcgagatgt ggaaacggcg aggcacttca
    16981 cattttttcg gattattgca aatgaggata aaaaaaccgg gtttccccgg tttcagagtg
    17041 atgataaaag caaaattgcc tgatgcgcta cgcttatcag gcctacattt ccttgcaata
    17101 tgtgcattac tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaaagcgc
    17161 aatttgccag caatagtgaa ttacggcttc gccacaaaac caatcgcttc gtacaccgct
    17221 ttcagcgtac gagaagcgtg tgcactggct ttttccgcgc catctttcat cacctgttgc
    17281 aggaaggctt cgtcattgcg gaaacggtga tagcgttcct gcaattcagt cagcataccg
    17341 gaaacggcat cagccacttc gcctttcaga tgaccataca tcttgccttc gaactgtttt
    17401 tccagttccg ggatgctctg gcccgttacc gcagaaagga tatccagcag gttggaaacg
    17461 cccgctttgt tctgcacatc gtagcgaact accggcggct cgtcggagtc agtgaccgca
    17521 cgtttgattt tcttcactac cgatttcgga tcttccagca ggccgataac gttattgcgg
    17581 ttatcgtcag acttggacat ctttttggtc ggctccagca gcgacattac gcgcgcgcca
    17641 gatttcggaa taaacggctc cggcacttta aagatctcgc catacagcgc gttgaaacgc
    17701 tgggcgatat cgcggctcag ttccaggtgc tgtttctggt cttcaccgac cggtaccaga
    17761 ttagtttgat acagcaggat gtccgcagcc atcagcaccg gatagtcaaa cagaccagca
    17821 ttgatgttct cggcataacg cgcagattta tctttaaact gcgtcatgcg gctcagttcg
    17881 ccgaagtagg tatagcagtt cagtgcccag cctaactgtg catgttccgg cacgtgggac
    17941 tgaacaaaaa tggtgctttt ctcaggatcg ataccacaag ccagatacaa ggccagcgta
    18001 tccagcgtcg ctttacgcag cttctgtgca tcctggcgca cggtgatcgc gtgttggtca
    18061 acgatacagt aaatgcaatg atagtcatcc tgcatgttta cccactgacg cagcgcaccc
    18121 atgtagttac caatggtcaa ttcacctgag ggctgtgcgc cactaaaaac gatgggctta
    18181 gtcatttttc gattcctgat tttcgctatg cggaagccct aatgcgggca gaaggtcatt
    18241 tatagactga taaattacat caggctggct gagatcgata gcctcgccgt agttatatcc
    18301 gtaggttaag ccaactgatg ggcaacctgc cgcttttgct gcctgaatat cattgcgtga
    18361 gtcgccaaca aacagcatct gttgtggggc aattcccatc cgctcagcca ccagtaacag
    18421 cgggtccgga tgcggttttt tgttttgcac atcatcaccg ccgataacca cgctgaagta
    18481 tttggcgata tctaaggctt cgagcagcgg cgcgacgaac ggcgtcggtt tgttggtgac
    18541 caggcctagc ggcaggcctt tagcctgcaa tgcgcccagc gtatcggcaa cgtgcgggaa
    18601 caaaaacgtc ccctcttcgg caacctcgcc atagtagcga tcgaacagtt tacgcagaat
    18661 acgtacctgt tcttctgccg gaatgtcgtc atcaacgggc ggtttaccca ttgttttacg
    18721 cagagtcgca cgttcctgac gcgcccaggt caatgcgcgc tccatcagaa tatctgcgcc
    18781 gttaccaatc caggtaataa cgcgttcttc acctgcgaca ggcaactcca gcgcatacag
    18841 cgccatatct accgcagcag caagaccggg tgcactgtca accagcgtac cgtcgagatc
    18901 aaaagcgacg ccgcgaatat cttcaaactt attcatgact tacctttgcc agttcactgc
    18961 gcatttcatc aatgactttt ttgtagtctg gctggtcgaa gattgccgaa ccagcaacaa
    19021 acatatccgc gcccgccgca gcgatttcgc caatgttgtt caccttcacg ccaccgtcca
    19081 cttccaatcg aatgtcaaag ccagactcgt cgatacgacg acgtacttcg cgcagtttat
    19141 ccagtgtttg aggaatgaaa gactgaccgc cgaaaccagg attgacggac atcagcagga
    19201 tcacatccag cttatccatc acgtaatcca gataactcag aggtgtcgcc gggttaaata
    19261 ccagacccgc tttacagcca ttttctttaa tcagttgcag cgtgcggtca acatgctcgg
    19321 aggcttctgg atgaaaggta atgatgctgg caccagcggc agcgaaatca ggcacaatgc
    19381 gatcgacggg tttcaccatc aggtgtacgt cgataggggc ggtaatgccg tagttacgca
    19441 aggatttcag caccattggc ccaatagtca gattgggaac atagtggtta tccatgacgt
    19501 caaaatgcac gacatcagcg ccagctgcca gggcttttgc ggtatcttca cccaggcggg
    19561 caaaatcagc cgacagaatt gagggggcaa tcaaatactg tttcatccgc ttctccttga
    19621 gaattatttt ttcgcgggtg aaacgactcc tggtttgtac aaagccagca gttcgtccac
    19681 ctttttacgt gtgccgccgt tgctgcttat actgcgtcga actttgacga catgcaattt
    19741 tgcgcgctga taccactcac gcgttaacat cgtatcgtga ttggagatca gcactggaat
    19801 atggcgatca accagacctt cggcgatctc cgccagatgc gcttgttgtt caagcgtaaa
    19861 actgtttgtg tgatacgccg taaagttggc ggtcgcagac agcggcgcat aaggcggatc
    19921 gcaatagacg acggatgcat catctgcgcg cgccatgcta tcggcgtaag actcacagta
    19981 gaaaaaggca ttctgcgctt tttcagcgaa gtgatacaac tctgcttccg ggaaataggg
    20041 ttttttgtag cggccaaacg gcacgttaaa ctcaccgcgc agattgtaac gacacagacc
    20101 gttgtaaccg tagcggttca aatataaaaa cagtaccgcc cgacggaacg gatcctggct
    20161 tttgttgaac tcttcgcgga actgatagta aacctcggcg caatttgttt cgggaacaaa
    20221 cagctcgcgt gcggcctgta cgtactcatc agtacgcatc ttcacaatgt tatagagact
    20281 gatcaggtcg ctattgatat cggcaaggat ataacgagaa aagtcggtgt tgagaaacac
    20341 cgacccggca cctacaaaag gctcaaccag acattcgccc ttgggcaaat gccgtttaat
    20401 atcatcaagc aggggatact tgccccctgc ccacttcaaa aaagcgcgat ttttcttcat
    20461 gctgactaac taattacacc ttctccggct gtggagaaag ctccgacagc atccagcgct
    20521 tcaggcaact cccgcgatga taaaccattg cgggagataa ccttgattac ttcagatcgg
    20581 cctgtacctg acgcagcggt ttcgcccacg ggtttttggc ctggacatcg gctggcaatg
    20641 tagataccgc ttttttcgcc tcttctttcg aagcgtacac gccagaaacc aggacatacc
    20701 acggctgacc attacgcgtc gtttcataga caacgtagtt tttcagattc tctttcttcg
    20761 cccaaccgtt caggttgtcg tagttagagg aactgctcag ctgcagagtg taatggctgg
    20821 acggtgccga tttcaacgaa ccaacattac ctgcggtctt ccctccagcg gctggtgttg
    20881 ccgtggtttg cgccggggat gccgtctgta ctggagccgt agtcgccgtc tcttttggtg
    20941 ctggcgtaga agtcgcagcc ggtgccttcg tgcttgccac aggagcagct ggttcagtac
    21001 gcttcggcgt ctgtgctacc ggcttcggct ccgttttcac ggttgcttgc ggttttttcg
    21061 gttcaatcac cgcctgctgg cgagctggac gcgtagtggc cggacgttca gcggtttgcg
    21121 ttttcgccgt gtcacgcgat gcattgccat tgcgaacagg cgcgaccgtc gcgggttcag
    21181 tcggcaacgt ggaattgacc gccacattat tcaactgttg ctgattttgt ggctgggtca
    21241 gcgcattgtt caggtcaccc tgcacttcaa cacgttgttg accatccgtt gccgccgggg
    21301 tttgcccttg agtcggcgta gaagagatcg gcggcagaga aacatcctgc tgagtatttt
    21361 ccgcagacgt ggttcccggc gctggctgca cgccattcgc ctgatcggtc gcattgccag
    21421 caagatcaat actcttctcg ccagacgcgg tttgatcgct ggaagaggtc gagggggctt
    21481 ttagcgcaga accgataccg atgatcaaca gcaggagaac cagaatgccg acgcccatca
    21541 tcatatactg acgagaagcg ggtttactgg ctgctttttt gcgcttacgt ggacgacgct
    21601 ctacgcgctc ttcatccacg gtttcatctt cggattcgtc aatttcttct tcgagttccg
    21661 gttcctcatt gcgctctttt tgcgcacgag tcggacggcg atcgtcagtg tcatcaagtt
    21721 caatatcatc aaaattgatc tgcggttcgc cacgttcagt acgctcagaa cgttcagaag
    21781 attgacgaga acgaccagta cgacgatcgc tgggatcggg tttcagctcg tcttctggtt
    21841 tgaattcatc catttaacac cccactaaaa ggttaatgct taccacgttg caattaacct
    21901 gaagctaata gaccgcttga taagcggcct gacctttctt gttgttacgc tgattgacaa
    21961 tcggcaatgg cgttaagaac aagctcgtgc gaaacgccgc tgcgaacttc actcttacca
    22021 attgccaacg gaagaattaa gcgcatctct cccgcaagga ctttcttgtc acgcagcata
    22081 tgcggtaaat acgcctgcgc agacatttcg cgcggcccat tgaccggtaa cccagcccgc
    22141 gtgagcaggg ttataatacg ctgcgtttcg gcagaactaa actgcccgag acgttccgac
    22201 gtccgcgccg ccatcaccat acccgcagca accgcttcac cgtgtaacca attgccatac
    22261 cccatttcag cttcaatggc atgaccaaag gtgtgtccca gattcagtaa agcacgtaac
    22321 ccggtttcgc gctcgtcggc ggcgacaact tctgccttca gttcacaaca acggcgaata
    22381 cagtacgcca ttgccggacc gtccagacgc aacaacgcat ccagattctc ttccagccag
    22441 ttgaaaaacg caccgtcaag aataatgccg tatttgatga cttctgccag ccccgacgct
    22501 aactcacgcg ggggaagcgt tttcagacag tcgagatcca ccaccactga agcaggctgg
    22561 tagaacgcgc caatcatgtt tttaccgagg ggatggttga ccgcagtttt gccgccaacg
    22621 gaggaatcga cctgcgacag caatgtcgtc gggacttgaa tgaaacgtac accgcgctga
    22681 taactcgccg ccgcaaagcc ggtcagatcg cccactacgc cgccgccaag cgccaccagc
    22741 gtagtatcgc gaccatgcgg cttttgtaac aacgccgtaa agacggtatc gagtacagcc
    22801 aggcttttat actgctcgcc gtcagggagg ataacgctat cgacgttaac acccgcctgt
    22861 tcaagtacgc cgcggacctt atcgagatac aggggagcca gggtttcgtt ggtaaccaac
    22921 atgacctgct cgcccgattt cagcggtaag aatgaagctg gttcattaaa caaaccagat
    22981 gcgatggtaa ttgggtaact acgttccccg agagtaacga caatcctctc cataacgcga
    23041 catccacctt aattactgta cccgcagacg agtgtatata aagccagaat tagttgcttt
    23101 ccagcatgtg aataatctgg tttgcaacca ctttagcgct ttgatcatca gtacgaatgg
    23161 tcacgtcggc aatctcttca tacagcggat tgcgttcatt ggccaacgct tccagaactt
    23221 cacgcggcgg tgtttcaacg tgcagcaacg ggcgtttttt atcacgctgc gtgcgtgcaa
    23281 gttgcttttc gatggtcgtt tcaagataaa cgacaacgcc acgagcggaa agacggttac
    23341 gcgtttcacg ggatttcaca gagccgccgc cagtagccag cacaataccc tgtttctcgg
    23401 tcaactcatt gatgaccttt tcttcgcgat cgcggaagcc ttcttcgcct tctaaatcga
    23461 aaacccagcc cacatcagct ccggttcgtt tctcaatctc ttgatcggaa tcgtaaaatt
    23521 ccatattgag ttgttgagct aactggcgcc caatagtgct ttttccggca cccataggcc
    23581 caaccagaaa gatattgcgt ttctctgcca ttttttcggt actactaaga ctattcgtta
    23641 atgataaacc cgcttcgctc agagagcgcc gcaggacatg aactgaaacc tcataagata
    23701 ttgcgagagt cagactgaaa attatctcaa tactccagcg ggtttggcaa ctgaataaat
    23761 cacccatccc tctgcatatc tggtcgctgc aagcgcgctg ccttgctacc accgctctgg
    23821 cgattaatca ccgggtaaga ttagcgtaaa aaagacagca aaatgccgcc tgaatgataa
    23881 atcatcatca tggaacgcag gcagcgaaga gcaccaactc tcaaatcggt actccttgta
    23941 tgctaaatac ctgcgcgcgt caaatagatg aaacacgttc agcgtaaaaa cattaccgct
    24001 tttacggctg tttactcact ggaaaccagt cgtggcgtga taaacaccac taactcgcgt
    24061 cgttcatctt cttttccgtc atgacgaaat aattgcccga accagggaat gtcgccaagc
    24121 aacggtacgc tatcctgacc cgatttattt ttacgggtaa aaatgccgcc cagcgccaac
    24181 gtttctccgc ttttgacctc gacctgcgtt tcgatctcct gcttatcaat cgccaacact
    24241 tcgccatcgg cctgctgtag cacctgcccc ggaacgttct ggctgatgtg taatttcagc
    24301 cggatgcgac ctttttgtaa caccgtgggc gtaacttcca tccccaggac ggcctcttta
    24361 aattccaccg acgtcgcgcc actttccccg ctggaaacct gatatggaat ttcgctcccc
    24421 tgtttaatgc tggcaggctg aagatgtgag gccagcagac gcggactggc gataatatcc
    24481 agctgctgtt tttgttcgag cgcggaaagc tcaagatcca gtaaacgtcc gttgatgcgt
    24541 ccaatgttaa aaccgacatg cgttgtcgcc gtcgctacgg agaggtcgct gccaagcgtg
    24601 gtgacttgcc caacgccacc agcttgctgc gcatcagcca acgtccattt cacgcctaac
    24661 tcacgcaaac ttttttcatt aatggtgaca atatgcgccg acagctcaac ctgcccgacc
    24721 ggcagatcca tttgcgctac ccactgttca agcgcgctta acgccgtttt gttatctcgc
    24781 agcaaaaggc gattggtgcg tttatcgacg gtcatactcc ctttggcact cagtagcttc
    24841 tccccggctt tcgccagttc tcccgcgtcg gcgtattgca gggttatact gcgattttcc
    24901 agcggcagat ttgcctgcgc ccgcgcctgc tccgcctcct gacgggcgat attgtcattc
    24961 tgccaggcaa cggaatgcac cgagagaatg ttgccctcct ggcgcgttat caatccggcg
    25021 cttttcacta cagtttgtag tgcctgcttc cagggaacgt cagttaaatg taacgacacc
    25081 gtaccgctga cgtctggcga aaccaccagg ttcaacttct cctgttcagc cagcgcctgc
    25141 aacacctgag ctaccggaac gtcatccacc atcagcgtca ctttttgcgg ctttgccgcc
    25201 tgtacgccgg gtataagcat caacagtagt gcggctatcc attgcttcat ttgtatctcc
    25261 ttgccgttgc cacaaccagt gtggcggttc gcagtttgtc ccggttccca gcgttagtgc
    25321 gtctggcgtc agctgtaaaa ttgtccagcc gttttccagc acgtcgtttt gctgcacccg
    25381 tcgccatttt ttttgcccgt cttttattac accgatgatg cgctcacctc gccctaccat
    25441 cccctgatag cgccactggc taagttcgct aatccggcat agatcttccg gcggtttaaa
    25501 agggtcacgc ataccggtta aaaggcacaa tgtaatacct gccaacaacc agcgtttaac
    25561 cctcatgcgg cgtctccagt tgtagcgtga acaaaaggtc atcaccttcc acgcttaacg
    25621 aaaaacggct cacgctgacg ttgcgctctg ccagccgtgt aaatgccgac ggcactgctt
    25681 cccacagcgt tttcaacgcc aactcacctc cctgcgcgga tggatgccag taaaccagtt
    25741 gcgcgccgga taactgaaaa tccagtggcg aaaagggcag cgttttttcc tcgctgaagg
    25801 gagtggtgtc aaccaggcga tacagtgcgg cccactgctg gtgatgactt gcccgtagtc
    25861 gaattaatgc gtcgctctct tcatggtgtg tcgatgacag aaaaatgagc gtaactaaca
    25921 tcagcaacca gactgcccag caaagctggc ggaggcgggg tgatgtggcg aaccaccagt
    25981 caaagagcat gtccatcgct aaccttcctt gttaactgat actcaaattg ccagcgtccc
    26041 tgcgcatcct gctgcgtggc cccccgctga ttgagatgaa aagatgcatc ctggcggagt
    26101 gacgtttcca gcgcgtttaa cgcggtaatg cttgttgtca gccccttgat ctccagcgta
    26161 ccttgctgcc agcttatcgt tgtcagccag gcatgttcgg gtaaaagcgc cgccagtgct
    26221 tccagcgcag attgccagtc acgggtaaat tgtcgctgga gctgcctttg caaacgctgt
    26281 tcgcgtaact gctggcgttc cagcaaacgt ggtttcgtta tctgtaagct gcgggcgagt
    26341 tgttgttccg cctgaagcaa aacggtgtct acgcgtgctt cggcgttgct tgtcagacgc
    26401 agtattagcg ttatcccgac agccagcagc agaggcgcaa cgaacatcag caaccagaaa
    26461 cgcagaaaag cggtccggcg ttgctgtcgc cagggcaaaa aattaattgg cgggttcatc
    26521 agtacgcctt ccctagcgcc agccccagcg cgatggcaaa gtctccacct ggcggcggta
    26581 gcggcggctg acgaacagaa accgcctccc agggatcaaa tccgccttcg ccacatatcg
    26641 cgacgctttc gggatcaacg gataacgctg ccgccagctc cttcgcgcta gtcatcccca
    26701 ccgccagttt gcgcccccag cgatagcgcg tcgcccacag ccactgttcg ttatcacgcc
    26761 aggccagaca ttgctgatga gaaggtaaaa aaggcaggaa tcgctgtaat gcactggcat
    26821 ccggggtgat cgcgctaaca tgaacacgca acctttctgc cagagtaagc agcgttgcca
    26881 actctttgct ttgcgcggca gtcacgttat aggcgggact gagtgagtct tcgctgtaat
    26941 cgaagcgcag ggagtccgga tccatatcca gctcgcgggc catcgtcccg gacagccagg
    27001 ccgtttgctc ccgctcacca agggacatcg acggacgtgg aaatgaccgc tgtaatgtgc
    27061 gactggcggg aaacgccaac ataatgtgat gacgctgcgg cagttcgcga ctccacggta
    27121 acaacgtttt agccagccgc tgcacatcaa caatccgccc atctttgata atgtcgttct
    27181 ccagcggcaa ccgccaccag cgttgcaaaa agcattcttt tgtgccccaa ccgctaccgc
    27241 ttcttgctgt tgtaaatgca aaccaatttg ccagatctta aatgccattg tgatgatctc
    27301 cttatcaccc gtcactctga cgggtatatc aatgcgtctg gcttgccttt atactaccgc
    27361 gcgtttgttt ataaactgcc caaatgaaac taaatgggaa atttccagtg aagttcgtaa
    27421 agtatttttt gatccttgca gtctgttgca ttctgctggg agcaggctcg atttatggcc
    27481 tataccgcta catcgagcca caactgccgg atgtggcgac attaaaagat gttcgcctgc
    27541 aaattccgat gcagatttac agcgccgatg gcgagctgat tgctcaatac ggtgagaaac
    27601 gtcgtattcc ggttacgttg gatcaaatcc cgccggagat ggtgaaagcc tttatcgcga
    27661 cggaagacag ccgcttctac gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg
    27721 caagtgtggc gctgttctcc ggccacgcgt cacaaggggc gagtaccatt acccagcagc
    27781 tggcgagaaa cttcttcctc agtccagaac gcacgctgat gcgtaagatt aaggaagtct
    27841 tcctcgcgat tcgcattgaa cagttgctga cgaaagacga gatcctcgag ctttatctga
    27901 acaagattta cctcggttac cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg
    27961 gtaaaacggt cgaccaactg acgctgaacg aaatggcggt gatagccggg ctgccgaaag
    28021 cgccttccac ctttaatccg ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg
    28081 tactgtcgcg gatgctggat gaagggtata tcacccaaca acagttcgat cagacacgca
    28141 ctgaggcgat taacgctaac tatcacgcgc cggagattgc tttctctgcg ccgtacctga
    28201 gcgaaatggt gcgccaggag atgtataacc gttatggcga aagtgcctat gaagacggtt
    28261 atcgcattta caccaccatc acccgcaaag tgcagcaggc cgcacagcag gcggtacgta
    28321 ataacgtgct ggactacgac atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga
    28381 aagtgggcga gtcggcgtgg gataacaaca agattaccga tacgctgaag gcgctgccga
    28441 cctatggtcc gctgctgcct gccgcagtca ccagcgccaa tcctcaggaa gcgacggcga
    28501 tgctggcgga cgggtcgacc gtcgcattga gtatggaagg cgttcgctgg gcgcgtcctt
    28561 accgttcgga tactcagcag ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg
    28621 gtcagcaaat ctgggttcgt caggttggcg atgcatggtg gctggcgcaa gtgccggaag
    28681 tgaactcggc gctggtgtcg atcaatccgc aaaacggtgc cgttatggcg ctggtcggtg
    28741 gctttgattt caatcagagc aagtttaacc gcgccaccca ggcactgcgt caggtaggtt
    28801 ccaacatcaa accgttcctc tacaccgcgg cgatggataa aggtctgacg ctggcaagta
    28861 tgttgaacga tgtgccaatt tcccgctggg atgcaggtgc cggttctgac tggcagccga
    28921 agaactcacc accgcagtat gccggtccaa ttcgcttacg tcagggactg ggccagtcga
    28981 aaaacgtggt gatggtacgc gcaatgcggg cgatgggcgt cgactacgct gcagaatatc
    29041 tgcaacgctt cggcttcccg gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt
    29101 cagcgtcatt caccccaatg caggtggcgc gcggctacgc ggtcatggcg aacggcggct
    29161 tcctggtgga cccgtggttt atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg
    29221 aagcgaaacc gaaagtagcc tgcccggaat gcgatattcc ggtgatttac ggtgatacgc
    29281 agaaatcgaa cgtgctggaa aataacgatg ttgaagatgt cgctatctcc cgcgagcagc
    29341 agaatgtttc tgtaccaatg ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga
    29401 ccggcgcgca ggaatacgca ccgcacgtca tcaacactcc gctggcattc ctgattaaga
    29461 gtgccttgaa caccaatatt tttggtgaac ccggctggca gggtactggc tggcgagcag
    29521 gtcgtgattt acaacggcat gatataggcg gaaaaacggg taccactaac agttcgaaag
    29581 atgcgtggtt ctcgggttac ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg
    29641 atcaccgtcg taatctcggt catacaacag cttccggagc gattaaagat cagatctcag
    29701 gttacgaagg cggtgccaag agtgcccaac ctgcatggga cgcttatatg aaagccgttc
    29761 ttgaaggtgt gccggagcag ccgctgacgc cgccgccggg tattgtgacg gtgaatatcg
    29821 atcgcagcac cgggcagtta gctaatggtg gcaacagccg cgaagagtat ttcatcgaag
    29881 gtacgcagcc gacacaacag gcggtgcacg aggtgggaac gaccattatc gataatggcg
    29941 aggcacagga attgttctga ttaaaaaggc gcttcagcgc ctttttaagt ttgctgacaa
    30001 agtgcacttt gtttataccg gatacggcgt gaacacttta cccgtctaaa aacatctaga
    30061 aaaatcaata tattgcagga gccacacagg cctgataagc attatgcatc aggcaaactt
    30121 cacgcattta caaccgcccc tgccccttca accattcgcg cacgaggaaa agcgcgctga
    30181 cattacgcgc ttcgttgaag tcagggtctt ccagcaaatc catcatatgc gccagcggcc
    30241 agcgcacctg tggtagcggc tctggctcat cgccttccag cgattccggg tagagatctt
    30301 gcgctaccac gatattcatt ttgctggaaa agtaagacgg tgccatgctg agcttcttca
    30361 aaaaagtcag atcgttcgca ccaaatccca cctcttcttt tagctcgcgg ttcgcggctt
    30421 cgaagacgct ttcacccgga tcaattaatc ctttcgaaaa acctaattcg taggattcag
    30481 ttcccactgc gtattcgcgg atcaggatca ggtgatcgtc cacaatcggc acaatcatca
    30541 ctgcttcccg gttggttgga cgcattcgtt cataaacacg ccgcacgcca ttgctgaact
    30601 ccagatccac gctttcgacg gtaaacagtc gggaacgggc tacagtttca acattcagaa
    30661 tggtgggttt ttgtaatgat ttgctcatcg tgggatctat gctgtgaaat cagcggttat
    30721 tgtgcgatat cggacacgct ttcggcaatg tgaattgcat gttatttaca tttatgtaac
    30781 ttaataaata atcgtcctca aatcaaatta aaagtcaata ggttgaaata actccaggaa
    30841 tttgctgata ttccgccttc agagggtttg ctatgatcag cggttactgt gatgtgctta
    30901 atgatgctca agttaaactc cacgcttgcc gatagccaac cgcagaatca tgttttgtgt
    30961 ccggtgcgac tgaccacgcc tgacagacta agtaagatgg ggaaagcatg agcaccattg
    31021 tgattttttt agctgctttg ctggcctgct cactacttgc gggatggctg ataaaagtgc
    31081 gatccagacg gcgtcagctg ccctggacca acgccttcgc ggatgcgcaa acgcgtaaac
    31141 tcacacctga agaacgtagc gccgttgaaa attatcttga gagcctgacg caggtattac
    31201 aggtgcctgg cccaacggga gccagcgcgg caccgatttc tctggcgctg aatgccgaaa
    31261 gcaacaacgt catgatgcta acacacgcta tcacgcgtta cggcatctct accgacgatc
    31321 cgaataaatg gcgttactac ctcgattcgg tagaagtcca cctgccccct ttctgggagc
    31381 agtacatcaa cgatgagaat accgttgaac tgattcatac cgattcgctg ccgctggtta
    31441 tttcactcaa cggtcatacg ctgcaggagt acatgcagga aactcgcggc tatgccttgc
    31501 aacctgttcc gtcaacacag gcgtcgattc gcggggaaga gagtgagcaa atcgagctac
    31561 tcaatattcg caaagaaacg cacgaagagt atgcgctaag tcgtccgcgc gggctgcgcg
    31621 aagcgttgct gattgtcgcc tccttcctga tgttcttttt ctgcctgatt accccggatg
    31681 tatttgttcc gtggctggca ggcggcgcgt tactgctgct gggcgcaggt ctgtgggggc
    31741 tattcgcgcc cccggcaaaa tcctccctgc gggaaattca ttgtctgcgc ggtacacccc
    31801 gtcgttgggg attgtttggc gaaaacgatc aggaacagat caacaatatt tcactcggta
    31861 ttatcgacct ggtctacccc gcacactggc agccctacat tgctcatgat ctcggccaac
    31921 aaaccgatat cgatatctat ctcgaccgcc acgtagtgcg tcaggggcga tatctttcgc
    31981 tgcatgatga agtaaaaaac tttccgttac agcactggct gcgcagtacg attatcgctg
    32041 cgggttcgct gctggtgctg tttatgctgt tattctggat cccgctggat atgccgctga
    32101 aattcactct ctcatggatg aaaggcgcgc agaccattga agccaccagc gtgaaacaac
    32161 tggctgacgc tggcgtacgg gttggcgata cattgcgtat tagcggtacg ggaatgtgta
    32221 atattcgcac ttccggtacc tggagcgcga aaaccaactc acctttttta ccgtttgact
    32281 gctcgcagat catctggaat gacgcccgct cattgccatt accagaatct gaattggtca
    32341 acaaagcgac ggcattgacc gaagcagtta atcgccagct gcaccctaaa ccggaagatg
    32401 aatctcgcgt cagcgcctca ttacgttcag caattcaaaa atccggcatg gtattgcttg
    32461 atgattttgg cgacatagta ctgaagacag cggatctgtg ttctgccaaa gatgactgtg
    32521 tgcgactgaa aaatgcgctg gtcaatctcg gcaacagtaa agactgggac gcgctggtaa
    32581 aacgcgccaa cgccgggaaa ctcgatggcg tgaatgtgtt attacgcccg gtgagtgcgg
    32641 aatcgctgga taacctggtg gcaacctcca ccgcgccgtt tatcacgcat gaaacggcgc
    32701 gagcggcaca atcactaaac agtccggccc ccggcggatt cctgattgtc agcgacgaag
    32761 gcagcgattt tgttgatcag ccctggcctt cggcatcact ttacgactac ccgccgcaag
    32821 aacagtggaa cgctttccag aaactggcac aaatgctgat gcatacgccg tttaacgccg
    32881 agggtatcgt cacaaaaatc ttcactgacg ccaatggtac gcagcatata ggccttcatc
    32941 cgatcccgga tcgttccggc ctgtggcgct atctcagcac cacgctgctg ctactgacga
    33001 tgctgggtag cgccatttat aatggcgtac aggcctggcg gcgttaccag cgtcatcgta
    33061 ctcgcatgat gaagattcag gcctattatg aaagctgcct gaacccacaa ctgatcaccc
    33121 cttcagaaag ccttatcgaa taacacgttt gcgcggcagg ttatgctacc ctgtcgcgca
    33181 aattgcttca ctctggagat ttccctcatg catatcaaca ttgcctggca ggacgtagat
    33241 accgttctgc tggatatgga cggcacgttg ctcgacctcg ccttcgataa ctatttctgg
    33301 caaaagctgg tgcctgaaac atggggcgcg aaaaacgggg ttacgccaca ggaagcgatg
    33361 gaatatatgc gccagcaata tcacgacgta cagcatacgc taaactggta ctgtcttgat
    33421 tactggagtg agcaactggg tctggatatc tgtgcgatga ccaccgagat gggaccgcgc
    33481 gccgtactgc gtgaagatac cattccgttt cttgaggcac tgaaagccag cggtaagcag
    33541 cgaattttgc tcaccaatgc gcatccgcac aacctggcgg taaaacttga gcataccggt
    33601 ctggacgcac accttgattt attactttcc acccacacat ttggttatcc gaaagaggat
    33661 cagcggttat ggcatgcggt ggccgaagct acgggtctga aagctgaaag aacgctgttt
    33721 attgatgaca gcgaagcgat tctcgatgct gccgcgcaat ttggtattcg ttactgcctc
    33781 ggcgtgacta atcctgactc cgggattgcc gagaaacagt atcaacgcca tccgtcactg
    33841 aatgactacc gccgcctgat cccctcgcta atgtgaagga gacgccatga aagaaaaacc
    33901 tgctgttgag gttcgactgg ataaatggct atgggctgcc cgtttttata aaacccgcgc
    33961 gctggcccgt gaaatgattg aaggcggtaa ggtgcattac aacgggcagc gcagcaagcc
    34021 gagcaaaatc gtcgagctga atgccacgct cactctgcgc cagggaaatg acgaacgcac
    34081 ggtgattgta aaggcgatta ctgaacagcg tcgccccgcc agcgaggcag ccttgctgta
    34141 tgaagagact gcggaaagtg tagagaaacg cgaaaaaatg gcgctggcac gtaaacttaa
    34201 tgccttaacc atgccgcacc cggaccgacg cccggacaaa aaagagcgcc gcgacctgtt
    34261 acgatttaaa cacggcgaca gtgaataact gtcacctgca agagagatga ttatgccgca
    34321 acatgaccaa ttacatcgct atctgtttga aaactttgcc gttcgcggcg aactggtaac
    34381 cgtttcggaa accctgcaac agatccttga gaaccacgat tatccgcagc ccgttaaaaa
    34441 cgtgctggca gaactgctgg ttgcgaccag cctgttaacc gctacgctga agtttgatgg
    34501 tgatatcacc gtacagctgc aaggcgacgg tccgatgaat ctggcggtta ttaacggtaa
    34561 caacaaccag cagatgcgcg gtgtggcgcg cgtgcagggc gaaattccag aaaatgccga
    34621 cctgaaaacg ctggtcggca atggttacgt ggtgatcacc atcaccccga gcgaaggtga
    34681 acgctatcag ggcgtggtgg gtctggaagg tgatactctg gcggcctgcc tggaagatta
    34741 ctttatgcgt tctgaacagc tgccgacgcg cctgtttatt cgcaccggcg acgtagacgg
    34801 caaaccggct gcaggcggta tgttgttgca ggtaatgcct gcgcaaaatg cccagcagga
    34861 cgactttgac cacctggcga cgctaaccga aaccatcaaa accgaagaac tgttgacctt
    34921 accggcaaac gaagtgttgt ggcgtttgta tcacgaagaa gaggtgacgg tttatgatcc
    34981 gcaggatgtg gagttcaaat gcacctgctc gcgtgaacgt tgcgccgacg cgctaaaaac
    35041 gctgccggat gaagaagtcg atagcatcct ggcggaagat ggcgaaattg acatgcattg
    35101 tgattactgc ggtaaccact atctgttcaa tgcgatggat attgctgaaa tccgcaacaa
    35161 cgcgtctccg gcagatccgc aagttcatta atgagtatgt ccggcagaga gggctctgcc
    35221 ggattttacg acgtaatggc ctgatgcgct tatcagacct acggattcaa tgcaatttat
    35281 cgcaggtaag ataatgtgcc gaatccaata aaaatcagat atgcgctgtc attcattcag
    35341 attcaaatcc agtctttcgc catccaccgt aagatcgcgc cacactttag gcttcgtccc
    35401 cagttttcca tcctccgcag ccatgagtaa tttttctttc gttattacag ccgggagtga
    35461 attcatcgta atctctttcc attctttccc agcaggatca ataccataga taatgtatct
    35521 ttcatcatta aaggcaaaca ggatccgctc tgccctgcca tcactattca gatcttgctc
    35581 taccagcacg caggcatctt tttcaatgca tatcattgcg ttatagcgat ttttcatcac
    35641 tgccgaccag aatatcgtat ccggttttcc agaacctgag gcaataaata cattatctgc
    35701 taatgctttt tccgatatct gctgctgaag acgttgtact ccatcaaatg ccatcagcag
    35761 accccgcctg cgtttcgggt ctttcatata ctcggcatcg cttttcagcg actcaagcgc
    35821 cgcacgtcca tagcgaccgc tctgttcgag catatagaga cttacctggt ctggcgtgtt
    35881 tttgccgctc tgataacgcg ccatatgact gttcacgcta atgcgcatac tgtccagcac
    35941 cggagaatta agaagcacca gtattaccaa caccaataac gaaaccgcaa ggttcacttt
    36001 gccctgaaga acaagggggt tttgcccttt acgccagacg atactgacaa aataccccag
    36061 cgaccatacc agtaacacca gcaccgccag cgcgccctgc aagcggtcga cagtccagcc
    36121 gtactgagcg acccgcagcc ataacgccca ggcggcgacg aacacataca gcggagcaac
    36181 cagcaaagcg gttttaatca ggcaacgcaa cggccccgtc cagggaagag acgctttttg
    36241 cggatcgcgg acaatagcca ttaagatcaa ttgcaaaaag gccagcgtta acagcaaccc
    36301 agcggcggag atgtggcgag aaatcgcgct caggcccgta aacggcaggg tgataataaa
    36361 catcagggtt agcaatgata ctaacggcag caaccccgtg gcgattaacg tgaacaactt
    36421 ttgaatagag tcgattaaac gtgactgtgt acgcgcgagg atcaccgcca gtgcggtaac
    36481 cagacctaac gtgagataaa taaaccagtc ggttgcaaaa aagagcgttt tgaaaaacgt
    36541 gatgccaaca agtttaaaca actctcccca cagtaaaagt accagccacg tcaggccatt
    36601 ggcgagaaaa atcaccagta atataagtac gttatgccat actgactggt aaaaatagcg
    36661 atagcggcta ctgtcattgc ggatgcgaag gctttgttgt atccacggta gcaacaacat
    36721 tgccatcagc agcagatagc aaccaaaatc ccaaagcgcc ttttctgctc tccaggggtt
    36781 catgccatca atttgccact ttagccaacc gctcatccct agcgtggcaa taaacaccag
    36841 cgccagccat ccccaaaggc gcttctgttt aaaggagatc acggagaaga gtaaaacgga
    36901 tgaaaacgcg acggtcgcag gcacgccata aacaatccaa ctgtgatttt ttccggcaag
    36961 ccaggcgatc agcaggtagc aaaccagacc ctgaagtaat ccggtagcaa tcatccccca
    37021 gcgtgttgcc ggtgaaagtt caacgttatc catgttagtt atccagaatc aaaaagtggg
    37081 ttaattattg catccgggcc gtagtatttt gcttttttca gaaaataatc aaaaaaagtt
    37141 agcgtggtga atcgatactt taccgattga atttgcatca atttcattca ggaatgcgat
    37201 tccactcaca atattcccgc catataaacc aagatttaac cttttaagaa cattttccac
    37261 acctaaaatg ctatttctgc gataatagca accgtttcgt gacaggaatc acggagtttt
    37321 ttgtcaaata tgaatttctc cagatacgta aatctatgag ccttgtcgcg gttaacaccc
    37381 ccaaaaagac tttactattc aggcaataca tattggctaa ggagcagtga aatgcgcgtt
    37441 aacaatggtt tgaccccgca agaactcgag gcttatggta tcagtgacgt acatgatatc
    37501 gtttacaacc caagctacga cctgctgtat caggaagagc tcgatccgag cctgacaggt
    37561 tatgagcgcg gggtgttaac taatctgggt gccgttgccg tcgataccgg gatcttcacc
    37621 ggtcgttcac caaaagataa gtatatcgtc cgtgacgata ccactcgcga tactttctgg
    37681 tgggcagaca aaggcaaagg taagaacgac aacaaacctc tctctccgga aacctggcag
    37741 catctgaaag gcctggtgac caaacagctt tccggcaaac gtctgttcgt tgtcgacgcc
    37801 ttctgtggtg cgaacccgga tactcgtctt tccgtccgtt tcatcaccga agtggcctgg
    37861 caggcgcatt ttgtcaaaaa catgtttatt cgcccgagcg atgaagagct ggcaggtttc
    37921 aaaccagact ttatcgttat gaacggcgcg aagtgcacta acccgcagtg gaaagaacag
    37981 ggtctgaact ccgaaaactt cgtggcgttt aacctgaccg agcgcatgca gctgattggc
    38041 ggcacctggt acggcggcga aatgaagaaa gggatgttct caatgatgaa ctacctgctg
    38101 ccgctgaaag gtatcgcttc tatgcactgc tccgccaacg ttggcgagaa aggcgatgtt
    38161 gcggtgttct tcggcctttc cggcaccggt aaaaccaccc tttccaccga cccgaaacgt
    38221 cgcctgattg gcgatgacga gcacggctgg gacgatgacg gtgtgtttaa ctttgaaggc
    38281 ggctgctacg caaaaactat caagctgtcg aaagaagcgg aacctgaaat ctacaacgct
    38341 atccgtcgtg atgcgctgct ggaaaacgtc accgtgcgtg aagatggcac tatcgacttt
    38401 gatgatggtt caaaaaccga gaacacccgc gtttcttatc cgatctatca catcgaaaac
    38461 atcgttaagc cggtgtccaa agcgggccac gcaactaaag ttatcttcct gaccgcagac
    38521 gcctttggtg tgctgccgcc ggtttctcgt ctgactgccg atcaaaccca gtaccacttc
    38581 ctctctggct tcaccgccaa actggccggt acagagcgcg gcatcaccga accgacgcca
    38641 accttctccg cctgcttcgg cgcggcattc ctgtcgctgc acccgacgca gtacgcagaa
    38701 gtgctggtga aacgtatgca ggcggcaggc gcgcaggcgt atctggttaa caccggctgg
    38761 aacggcactg gcaaacgtat ctccattaaa gatacccgcg ccattatcga cgccatcctc
    38821 aacggttcgc tggataacgc agaaaccttc actctgccga tgtttaacct ggcgatccca
    38881 accgaactgc cgggcgtaga caccaggatc ctcgatccgc gtaacaccta cgcctctccg
    38941 gaacagtggc aggaaaaagc tgaaacgctg gcgaaactgt ttatcgacaa cttcgataaa
    39001 tacaccgaca cccctgcggg tgccgcgctg gtagcggcag gtcctaagct gtaatcagct
    39061 gatatctcat tgacccctta tccaaacgga taaggggttt ttttatctaa ttagctacca
    39121 atatgtacac ggtacactta taattatcaa tagaccgcag gatgcattat gttcaccttt
    39181 attgaactac aaggatttag caaacgccgc cagttattgc tacctgatga tgagttccgg
    39241 gcttttcagg aactactcat tcaagatccc actgccggtg acattatcgc aggcacaggc
    39301 agttttagaa aaattcgctg gaaccgttct ggaatgggga aacggggtgg cattcggata
    39361 atttattaca acgtaacacg caatggccga atttatctgg cgttgattta tcctaaaaat
    39421 gaacaggatg atttaacaga agaacaaaga aaggcattga aactattgtc cgagaagctg
    39481 ctttaatgtt gaagctccac aaattccacc atggccgaaa tgtcaacgga ggcattgaaa
    39541 acagatgaaa gatgaattat ttgctgatct tcttgccagc gcagaagaaa tggtacgcat
    39601 cgaaaagggt gaagaaacgc ctgaaccaga gcacgtccat acttttagcg aaatcgatgt
    39661 aaaagcgatc cgtgaagcaa cgggtttacg tcagcaagat tttgctgtcg ccgtaggggt
    39721 aagttatgac ctggtcaaaa gctgggaaac caaacgccgt caacctaccg gagcaccccg
    39781 aaaattgctc ctgcttttgc aagcgaatcc tttcattatc aatcaattga aaacaatcta
    39841 aaaacaggag gcaccttcgc ctcccgtttt atttaccctt cttttgtcgt gccctgcgcc
    39901 cgcgttaccg gcactggcag ccaggcgcga atggaaagcc cgccccgctc gctgctgcca
    39961 agctccagca tgccgttatg gttatccacg atacgctgca caatcgccag ccctaatccc
    40021 gtgccgctaa tggtgcgcgc actgtcgccg cgaacaaacg gctggaacag gtgcttacgt
    40081 tgttccggcg caattcccgg accatcatct tccacctgga accaggcgcg attcggctcc
    40141 gttccgctgc tgactttgat ccagccattg ccatagcggg cagcgttcac caccatattc
    40201 gccaccgcac gtttgatcga cagcgggtgc attttcacct caatgctgcc ggggtaaagc
    40261 gcggtttcaa tttcccgctc atagccactt tcggcggcaa tcacctcacc gagcactgcg
    40321 ttgagatccg ccatttccat cggcatctcc tgcccggtgc gcaggtagtc gataaactgc
    40381 tcaatgatgg cgttgcactc ttcgatatct ttattgatcg attcggccag gtagccatcc
    40441 tgctcgctca tcatctcggt cgccaggcga atgcgcgtca gcggcgtacg cagatcatga
    40501 cttacccccg ccatcagcag cgtgcgatca tccgccagtt gcttaacacc agccgccata
    40561 tggttaaagg cgcgggtaac ggaacgcacc tccgaagcgc catattcacg cagcggcggc
    40621 ggaataatcc ctttaccgac ctgcagagca gcatgttcga gatcgaccaa cggtcggttc
    40681 tggatacgga taaacagcca cgccccgcct atcgccaata gcataatcgc cagcgtatag
    40741 cggaacagcg gagaaaaatc accctgatga atttcggtca gcggcacgcg cacccagata
    40801 ttgggcgaca gccaggtttt cagccagaca acaggcgaac ttttgttgac ctcaacgcgc
    40861 acttccgtcg ggccgcccag ttgctgcgcc atctgatggc ttaagaattc atagtgttgt
    40921 gcccaacgca gacctgcctc ttcggcagct tcgttggaat agagagagat ccccagctca
    40981 cggtagatct cccgacggaa agcgggaggc acaaccaact gcgtgccgtc ctccagttgc
    41041 agtttgtcgg tcatcaacat acgcacttcg tacgcgagga ctttattaaa ctgctggagg
    41101 ctcggcaaaa tcgcgaagtt cagcaccacc agataagtcg tcaccaggct gacgaacagc
    41161 aaggtgacga tgagcaataa cgtacgggca aatgaacttc gtggcgagaa gcgcaatcgc
    41221 ctcatgcttt agaaccgtcc ggtacaaaga cgtagcccag accccagacg gtctgaatgt
    41281 aacgcggatg cgccggatct tcttccacca tgcggcgtag acgcgaaatc tgcacgtcga
    41341 tggagcgttc cattgcggag tattcacggc cacgggcaag gttcatcagc ttatcgcggg
    41401 agagcggctc acgcggatgg ctgaccagtg ccttcagtac cgcaaactca ccgctggtga
    41461 gcggcatcgg ctcatcttcg cggaacattt cacgcgtacc gaggttaagt ttgaacttac
    41521 cgaaagcaat taccgcctct tcctgtgacg gcgcgcccgg cagttcgttg gcctgacgac
    41581 gcagcaccgc acgaatgcgg gccagcaatt cacgcgggtt aaacggtttt ggaatgtagt
    41641 cgtcagcgcc aatctccagg ccaacgatac ggtccacttc ttcccctttc gccgtcacca
    41701 taatgatcgg catcgggttg ctctggctac gaagacgtcg gcaaatcgac aagccatctt
    41761 caccaggtaa catcaaatcc agtaccataa gatggaaaga ttcacgagtc agcaggcgat
    41821 ccatctgttc tgcattagcg acgcttcgaa cctggaagcc ttgttcggtg agataacgtt
    41881 ccagcagcgc gcgcaggcgc atatcgtcat cgaccaccag aatcttgtag ttctcttgca
    41941 ttgtttgtac tcccaaaggt tcgcaacaat ttgtaagcgt gtattcttaa aaaagctcac
    42001 gttcgtcacc agctaaatct ggtatgaatt tcagcctaaa ttgttacaaa gcatattaaa
    42061 cagcagctta agtatacaat ttattcggcg aaacattatt gattctgttg atatgatcac
    42121 gttataccca atgtgcgcat tatcaaacag acaaagggaa tcaacgagat gaaaacgccc
    42181 ctggttaccc gggaagggta tgaaaaactc aaacaagagc ttaattatct ctggcgtgaa
    42241 gaacgcccgg aggtcacaaa aaaggtgacc tgggccgcaa gtctgggcga ccgcagcgaa
    42301 aatgctgact atcagtataa taaaaagcgc ctgcgtgaaa tcgaccgtcg cgtgcgctat
    42361 ctcactaaat gcatggaaaa tctcaaaatc gtcgattact cccctcagca ggaaggcaaa
    42421 gtcttttttg gtgcgtgggt ggagattgaa aacgacgatg gcgtgactca ccgtttccgt
    42481 attgtcggct acgatgaaat ttttggccgt aaagattaca tctctatcga ttccccgatg
    42541 gctcgcgcat tgttgaaaaa agaggtgggc gatttggcgg tggtgaatac ccctgccggg
    42601 gaagcgagct ggtatgtgaa tgctatcgag tacgtgaaac cgtaaggaag agtcttaacc
    42661 tcctgccgat ggctggcatt tttgccagcc agtccgtata actatcccct gatttttgat
    42721 ccgaaaagat gaactcaaac catgatgaat gattcgttct gccgcattat tgcgggtgaa
    42781 attcaggcgc gcccggaaca ggttgacgct gccgttcgcc tgcttgacga agggaatacc
    42841 gtgccgttta tcgcacgtta tcgtaaggaa atcaccggcg gtctggatga cacgcagctg
    42901 cgtaatctgg aaacgcgtct gagctatctg cgcgagctgg aagagagacg tcaggcgatc
    42961 ctcaagtcca tttccgagca aggcaaactc actgacgagc tggcgaatgc catcaacgcc
    43021 acactaagca aaaccgaact cgaagacctc tacctgccct acaaacctaa acgccgcacc
    43081 cgcgggcaaa tcgccattga agcagggctt gagccgttgg ctgacctgct gtggagcgat
    43141 ccgtcacaca cgccagaagt cgccgctgca caatatattg atgccgataa aggcgtggca
    43201 gataccaaag ccgcgctgga cggcgcgcgc tatatcctaa tggaacggtt tgccgaagat
    43261 gccgcgctgc tggcgaaagt gcgtgattat ctgtggaaga acgcgcattt ggtctcaacc
    43321 gtggtgaatg gtaaagaaga ggaaggggcg aaattccgcg actatttcga tcatcacgaa
    43381 ccgctgtcca cggtgccttc tcaccgcgcg ctggcgatgt tccgtggtcg taacgaaggc
    43441 gtacttcagc tttcgctgaa tgccgatccg cagttcgaag agccgccgaa agagagctat
    43501 tgcgagcaaa tcatcatgga tcaccttggc ctgcgcctga acaatgcccc ggcggatagc
    43561 tggcgcaaag gtgtggtgag ctggacgtgg cgcatcaagg tgttgatgca tctggaaact
    43621 gaactgatgg gcaccgtgcg cgaacgcgcg gaagatgaag caatcaacgt ctttgcccgt
    43681 aacctgcacg atctgctgat ggcggcccct gccggactgc gtgcaacgat gggcctcgat
    43741 ccgggtctgc gtaccggggt aaaagtggcg gtagtcgatg cgacaggtaa actggtggcg
    43801 accgatacca tttatccgca taccgggcag gccgcaaaag cagcgatgac cgttgctgcg
    43861 ctgtgtgaaa agcataacgt tgaactggtg gcgatcggca acggtacagc ttctcgcgaa
    43921 accgaacgtt tctatctcga cgtgcagaag cagttcccga aagtgaccgc gcagaaagtg
    43981 atcgtcagcg aagctggcgc gtcggtttac tcggcttccg agctggcagc acaggaattc
    44041 ccggatctcg acgtttcgct gcgtggcgcg gtgtctatcg cccgtcgttt gcaggatccg
    44101 ctggcggagc tggtgaaaat cgatccgaaa tctatcggcg taggtcagta tcagcatgac
    44161 gtcagccaga cgcagctggc acgcaaactg gatgcagtag tagaagactg cgtaaacgcc
    44221 gttggcgtcg atctcaacac cgcttctgtt cctctgttaa cccgcgtggc gggcctgacg
    44281 cgcatgatgg cgcaaaacat cgtggcctgg cgcgatgaga acggccagtt ccagaaccgt
    44341 cagcaactgt taaaagtgag ccgtctgggg ccgaaagcct tcgagcagtg cgcgggcttc
    44401 ttgcgcatta accatggtga taacccgctg gacgcgtcta ccgtccaccc ggaagcctat
    44461 ccggtggtgg aacgcattct ggcagcaaca cagcaggcac tgaaagatct gatgggtaac
    44521 agcagcgagc tgcgtaacct gaaagcgtct gactttactg atgaaaaatt cggtgtgccg
    44581 acggtaaccg acatcatcaa agagctggaa aaaccgggtc gcgatccgcg tccggaattt
    44641 aaaaccgctc agtttgccga tggcgtcgag acaatgaacg acctgcaacc gggtatgatc
    44701 ctcgaaggtg cggtgaccaa cgtcaccaac tttggcgcgt ttgtcgatat tggcgtgcat
    44761 caggacggcc tggttcacat ctcttcattg tcgaacaagt ttgtggaaga tccgcatacc
    44821 gtggtgaaag cgggcgacat tgtgaaggtg aaagtgctgg aagtggatct tcagcgtaaa
    44881 cgtatcgccc tgaccatgcg tctggacgag cagcctggcg aaaccaacgc ccgtcgcggt
    44941 ggcggtaacg atcgcccgca aaacaaccgc ccggcagcca aaccgcgtgg gcgtgaagcg
    45001 cagcctgccg gtaatagcgc gatgatggat gcgctggcgg cggcaatggg caaaaaacgt
    45061 taaacgcccg gactggcccc taaacctgcg cgattaggcg ttcacgccac atctggcaac
    45121 gaacgccttg cctgacataa aagtgccgga gaatatctcc ggccttttta ttccacaagc
    45181 aaactcataa tatatcccat taatatttat cacttcatta acaactgaaa ccttaattaa
    45241 acattaacca gccctaataa ttcgtcatgc gaattatatt ttcgctgcga tataaccttg
    45301 agccatatca acattgagtc agattattat tcaaaccaac attcacacac attttaagta
    45361 ttgctgatag aaaccattct cattatcatt gtactgttga ttatttaatc tctccttcgt
    45421 tggcaaatca tctggtctca tgtcgctgtc aaacgcccca tgaggtagtt atccagttaa
    45481 tgagaaacaa gtaggcacct atgcaataca ctccagatac tgcgtggaaa attactggct
    45541 tttcccgtga aatcagcccg gcatatcgcc aaaaactgct ttctcttggc atgttacctg
    45601 gctcctcttt taatgtggtg cgcgtcgctc cactcggcga ccccattcat atcgaaaccc
    45661 gtcgtgtgag cctggtatta cgaaaaaaag atctggcctt attagaagtg gaagcggttt
    45721 cctgttaata cggtgataac aacaatgaaa aaattaacca ttggcttaat tggtaatcca
    45781 aattctggca agacaacgtt atttaaccag ctcactggct cacgtcagcg tgtaggtaac
    45841 tgggctggcg ttaccgtcga acgtaaagaa gggcaattct ccaccaccga tcatcaggtc
    45901 acgctggtgg acctgcccgg cacctattct ctgaccacca tctcatcgca gacctcgctc
    45961 gatgagcaaa tcgcctgtca ctacattttg agtggcgacg cagacctgct gattaacgtg
    46021 gtggatgcgt ctaaccttga gcgtaacctg tacctgacgc tgcaactgtt ggaactcggc
    46081 attccctgca tcgtggcact gaacatgctc gacattgccg agaagcaaaa tattcgtatt
    46141 gaaattgatg ctctgtcggc gcgtcttggc tgcccggtga tcccgctggt ttcaacccgt
    46201 ggtcgcggta ttgaagcgct caagctggcg attgatcgct ataaagctaa cgagaatgtg
    46261 gaactggtgc attacgcaca gccgctactc aacgaagcag attcactggc aaaagtgatg
    46321 ccttccgaca tcccgctgaa acaacgtcgc tggctgggcc tgcaaatgct ggaaggcgat
    46381 atctacagcc gcgcctacgc cggtgaagcg tcgcagcatc tggatgccgc cctcgcccgt
    46441 ctgcgtaatg agatggacga tccggcgctg cacattgccg atgcgcgtta ccagtgcatt
    46501 gctgccatct gtgatgtggt aagcaacacc ctgacggcag aacccagccg tttcaccact
    46561 gcggtagata aaatcgtgct caaccgtttc ctcggtctgc cgattttcct ctttgtgatg
    46621 tacctgatgt tcctgctggc tatcaacatc ggcggggcgt tacagccgct gtttgatgtc
    46681 ggttccgtgg cgctgtttgt gcatggtatt cagtggattg gctacacgct ccacttcccg
    46741 gactggctga ctatcttcct cgcccagggc ctgggtggcg gtattaacac cgtgctgcca
    46801 ctggtgccgc agatcggcat gatgtacctg ttcctctcct tccttgagga ctccggctat
    46861 atggcgcgtg cggcgtttgt gatggaccgc ctgatgcagg cgctgggctt gccggggaaa
    46921 tcctttgtac cgctgatcgt cggcttcggt tgtaacgtac catcggtaat gggcgcacgt
    46981 acgcttgatg caccgcgtga acgcctgatg accatcatga tggcaccgtt tatgtcctgc
    47041 ggcgcacgtc tggctatctt cgcagtattc gcggctgcct tcttcgggca gaacggtgcg
    47101 ctggcggtct tctcgctgta tatgctgggt atcgtaatgg cggtgctgac gggcctgatg
    47161 ctcaagtaca ccatcatgcg cggtgaagcg acgccgtttg tcatggagct gccggtctac
    47221 catgtaccac acgttaaaag cctgattatc cagacctggc agcgtctgaa aggcttcgtt
    47281 ctgcgtgctg gtaaagtgat tatcatcgtc agcattttcc tgagcgcctt caacagcttc
    47341 tcgctgagcg gaaaaatcgt cgataacatc aacgactcgg cgctggcttc cgtcagccgg
    47401 gtgatcaccc cagtcttcaa gccgattggc gtgcatgaag ataactggca ggcaacggtt
    47461 ggcctgttta caggcgcaat ggcgaaagaa gtggtggtgg gcacgctcaa caccctctac
    47521 accgcagaaa atattcagga cgaagagttc aatccggcag aatttaacct cggtgaagag
    47581 ctgttcagcg cggtagatga aacctggcag agcctgaaag acaccttcag ccttagcgta
    47641 ctgatgaatc ccattgaagc gagcaaaggc gacggcgaaa tggggaccgg ggcgatgggc
    47701 gtgatggatc agaaattcgg tagcgcagct gccgcataca gctacctgat tttcgtcctg
    47761 ctgtatgtac cgtgcatctc ggtgatgggg gctatcgccc gtgaatcaag ccgtggctgg
    47821 atgggcttct ccatcctgtg ggggctgaat atcgcttact cactggcaac attgttctat
    47881 caggtcgcca gctacagcca gcacccaact tacagcctgg tgtgcattct ggcggttatc
    47941 ctgtttaaca tcgtggttat cggtttgctg cgtcgcgcgc gtagccgggt ggatatcgaa
    48001 ctgctggcaa cgcgcaagtc ggtaagcagt tgctgcgcag ccagcaccac cggtgattgc
    48061 cattaatggc ttcacttatt caggtgcgtg atttgctggc gttacggggc cgtatggaag
    48121 cggcccagat aagccagaca ttgaacaccc cgcagccaat gattaacgcc atgctgcaac
    48181 agctggaaag catgggcaaa gccgtgcgga ttcaggaaga acctgacggc tgcctctctg
    48241 gcaattgtaa aagctgcccg gaaggcaaag cctgcctgcg cgagtggtgg gcgctgcgtt
    48301 aaccatactc catcacctgt tttgaaaagc ccggtatgcg tttgcatccg ggcttttttg
    48361 cgtgcggctt tccataaaaa tgcaaccctt gcagcgcgac gtaaattcct ctgaaagcgt
    48421 ctcgcaggga tgaaaactcg ctaatgcaca ggtgtggagt ggtgcgtaga gtcgcggcat
    48481 tcaaacaaca ggtgaaggaa cgccatgagc aaaaagcaga gttccacccc acacgatgcg
    48541 ctgttcaaac tctttttacg ccaaccggag acggcgcgcg attttcttgc gtttcattta
    48601 ccggcaccta ttcacgcgct ctgtgatatg aaaaccctca agctggagtc gagcagcttt
    48661 attgatgacg atctgcgtga aagctattcc gacgtgctgt ggtcggtgaa aacggaacaa
    48721 gggccaggat atatctattg tctgattgaa catcaaagca cctcaaacaa actgatcgca
    48781 tttcgcatga tgcgttacgc cattgccgca atgcaaaatc acctggatgc cggatacaaa
    48841 acattgccga tggtggtgcc attgttgttt taccacggta ttgaaagccc ctatccctat
    48901 tcgctgtgtt ggcttgattg tttcgccgat cccaaactgg caaggcagct ttatgcctcc
    48961 gcatttccgc tgattgatgt caccgtcatg cctgatgatg aaatcatgca gcaccgacgt
    49021 atggcgctgc tggagttaat tcaaaaacat attcgtcaac gcgatctgat ggggctggta
    49081 gagcaaatgg cctgcttatt aagtagtgga tacgctaatg acagacaaat caaagggctg
    49141 tttaattaca tcttgcaaac cggcgatgcg gtacgtttta acgattttat cgacggcgtt
    49201 gccgaacatt caccgaaaca caaggagagc ttaatgacta ttgcggaaag attgcggcaa
    49261 gaggggtttc agaaaggtat acgagctatc gccaaaacca tgctggatgc cggagtccct
    49321 cttgaggacg ttctgcgatt taccagcctg acggttgaag accttgctga aataaatcat
    49381 caataacgta aaggatggtt ttaatgacag atgcagaaag agaatatctt attagttacc
    49441 tgattggtta tttattgggt cgacgcctgg aagccctgga tatcgccaaa agaatgctgt
    49501 aatccggagt ccctcttgca gacatcatgc gctttaccgg ggtgtcagaa gaagagttgg
    49561 ctgcggcgag ccagtaaagt tctgtctcgc catttcaaaa gccacctaca ccctctgctt
    49621 caacgccacc agcatgcgac aaaactcggc cggatgcgaa ataaacggcg catgggccgc
    49681 tttggcgaag atgtatgatt cgctgtgagg ccaaagttta tccagcatcg gcaccacttt
    49741 gcgcggcacc agaccgtcga gatagccata caatcgcaaa aacggcatgg acacgttttg
    49801 cagcggctga cggagatcga ccattttcag gatttccagc ccgccattaa gcacgtcaac
    49861 ctccggcatc ggtaacgcca gaacggtttt cttcagcgcc cgcgcatcct ggcgcgccgt
    49921 ttcagtaccc atggtttgta acgccaggaa ccgctccacc gtacgctgaa aatcatcact
    49981 gagttgctgc tgaaatcccg ccagcacgtc cggttttatc cccggccact cgtcacgagc
    50041 actaaaacaa ggtgacgacg ccacggtgac cagcgcctga acacgctcgg gatgggttaa
    50101 cgcaatctgg cttgccacca gcccgcccag actccagcct aaccaaatgg ctttatcagg
    50161 tgcctgtcgc agcacggctt cggccatatc agcaagtgac atcgcaccaa atccccggct
    50221 acgcccgaag ccgggcagat caacaaggtg cagcgtaaaa tgcgagctca gttcctcgtc
    50281 aatgcaacgc cacacttcgg cattcagtcc ccatccgtgc agcagcacaa gatgaacatt
    50341 cccctgacct ttggtctgcc accagatgtt attcatccgc tattgttctc ttttgactta
    50401 caaggatgaa catatgctaa cagtaccggg attatgctgg ctatgccgaa tgccgctggc
    50461 gttaggtcat tgggggattt gttcggtatg ctcacgcgcc gcccgtacag ataaaacgtt
    50521 atgcccccaa tgtggattac ccgccacaca ctcacatctt ccctgcggtc gctgcctgca
    50581 aaaaccgccg ccctggcaaa gactggtcac ggttgccgac tatgcgccgc cgttaagtcc
    50641 gcttatccac cagcttaagt tttcccggcg cagcgaaatc gccagcgccc tggcacgtct
    50701 gttactattg gaagtcttac acgctcgtcg caccaccggg ttgcaattgc cggatcgcat
    50761 tatcagcgtt ccgttatggc agcagcgtca ctggcgtcgg ggatttaatc agagcgattt
    50821 gctgtgtcag ccgttatcac gctggttgca ctgccgatgg gatagcgaag ccgtcacacg
    50881 aacacgggcc actgcgaccc agcattttct cagtgcccgg ctgcgcaagc gcaacctgaa
    50941 aaatgccttt cgccttgaat tgcctgtgca aggtcgccat atggtgattg tggatgatgt
    51001 cgtcaccacc ggaagtaccg tcgcagagat tgcgcagttg cttttacgca atggtgcggc
    51061 gactgtccag gtctggtgcc tttgtcgaac cttgtagagc ctcgatgatg ggcgtattat
    51121 aaccaactaa aatagtcaac tattaggcca ttactatgat ccgtatttcc gatgctgcac
    51181 aagcgcactt tgccaaactg ctggcaaatc aggaagaagg gacacaaatc cgcgtatttg
    51241 tgattaaccc tggcacgcct aacgctgaat gtggcgtttc ttattgtccg ccggacgctg
    51301 tagaagccac cgacactgcc ctgaaatttg acctgctgac cgcgtatgtt gatgagttaa
    51361 gcgctcctta cctggaagat gcagagatcg actttgttac agaccagttg ggttcccagt
    51421 taacgctgaa agcgcctaac gccaaaatgc gtaaagtcgc agacgatgca ccgctgatgg
    51481 agcgcgtgga gtatatgctg cagtcgcaga tcaacccaca gcttgctggt cacggtggtc
    51541 gcgtttcgct gatggaaatc accgaagacg gttacgccat tctgcaattt ggcggcggct
    51601 gtaacggttg ttctatggta gatgtgacgc tgaaagaagg gatcgagaag cagctgctga
    51661 acgaattccc ggagctgaaa ggtgtgcgcg atctcaccga acaccagcgc ggcgagcact
    51721 cctactacta agttatcccc tcttttgtag attgcccgat gcgacgctaa agcgtcattt
    51781 cgggcctacg ttgaacatgt gccagacgtt ggcgcagcat tgctttggca aacccctccc
    51841 ttcgtttccc cgcgataata tgaccaacct ctcataattt aaatttaccc cgctctggtg
    51901 attctcaaac gccggatgtt acccgtatca ttcacgtggg taccaaacat actcctgaca
    51961 tctgactaca ataattagtt ttagtgggta tcagtcgtgg tgccgcaata tctctgttcc
    52021 cgattgggat aattagagtt tgtcgtcaga aaattgacgt tacccataac aaatgaaagg
    52081 ccaggtaaat catgccatta gtcattgttg ctatcggtgt aatcttgttg ttgctcctga
    52141 tgatccgttt caaaatgaac ggcttcatcg ctctcgtcct cgtggcgctt gctgttggat
    52201 taatgcaagg aatgccgctg gataaagtta ttggctccat caaagcaggt gtcggcggaa
    52261 cgctcggtag ccttgccctg atcatgggtt ttggcgcaat gctgggcaaa atgctggcag
    52321 actgcggtgg cgcacaacgt atcgccacca cgctgattgc taaatttggt aaaaaacaca
    52381 tccagtgggc ggtggtactg accggtttta ccgttggttt tgccctgttc tatgaagtgg
    52441 gctttgtgct gatgctgccg ctggtgttta ccatcgcggc ttccgcgaat attccactgc
    52501 tgtatgttgg tgtaccaatg gcggctgcac tgtctgtgac ccacggcttc ctgccaccgc
    52561 atccgggccc gactgcgatt gccaccattt tcaatgccga tatgggtaaa accctgctgt
    52621 acggtactat tctggcaatc ccgaccgtga ttctcgccgg tccggtttac gctcgcgtgc
    52681 tgaaaggtat agataagccg atcccggaag gtctgtacag cgcgaaaacc ttcagcgaag
    52741 aagagatgcc aagttttggc gtcagcgtct ggacctctct ggtcccggtt gtgctgatgg
    52801 cgatgcgtgc gattgccgaa atgatcctgc cgaaaggtca cgctttcctg ccggtagcgg
    52861 agttcctcgg tgacccggta atggctacgc tgattgccgt actgattgcg atgttcacct
    52921 ttggtctgaa ccgtggtcgt tcaatggatc agattaacga cacgctggtt tcttccatca
    52981 aaatcattgc gatgatgctg ttgattatcg gtggtggcgg tgcgttcaag caggtgctgg
    53041 tagacagcgg cgtggacaaa tacattgctt ccatgatgca cgaaaccaac atttctccgc
    53101 tgctgatggc ctggtcgatt gctgccgtac tgcgtatcgc gctgggttcc gcaaccgttg
    53161 cggcaatcac tgcgggtggt atcgcggcac cgctgattgc aacgacgggt gttagcccgg
    53221 agctgatggt tattgcggtt ggttccggta gtgtgatttt ctctcacgtg aacgatccgg
    53281 gcttctggct gttcaaagag tactttaacc tgactatcgg cgagaccatc aaatcctggt
    53341 cgatgctgga aaccattatc tcggtgtgcg gtctggtagg ctgtctgctg ctgaatatgg
    53401 tgatttgata acactgactg ccggatgcat cgcatccggc atcaaccgca ctctacttct
    53461 tcttcgctgc cgctctgcgc cgtctgtcca aatccttcag caacttgttc acaccatcat
    53521 cggcaaacat cgcctcaagc gttgcggaga gcttgcgccg ccagttttta tactggtaac
    53581 tggtgccggg aatatttacc gggtccgcca tatccagcca gtcttctggc tgtagtccta
    53641 acagggcgct gttgctatcg gcgatgtagc gctgtaagcc acgattcagc gtcggtgtca
    53701 tcgacatcaa cgatgcctta tgcccggcac gttttggcag gcaaccatat ttatgcagag
    53761 catccagtag tccttgcttc gccagttcgc gatcctgata cagaccgcgc agtaccactt
    53821 catccggata cagccccagc gttttgccca gcgttaaatc cccgctctcc cagtagccgc
    53881 gtagcgtagg caggtcgtgt gtcgccgcaa ccgccatcga ctgctctggg tacgctttcg
    53941 gtgcgcggaa tgtcttctcg tggtcgtttt cgaaatagag cactttgtag gagtacacac
    54001 cgctgctgcg cagcttaccg acaatctcta ccggtacggt accgagatct tcaccaatca
    54061 ccatacagcg atgacgttta ctttcgagtg ccagaatcga gagcagatca tccaccggat
    54121 agtgaacata cgcgccctga tctgccgtct cgccatacgg tatccaccac aaacgcagca
    54181 tcgacatcac gtgatcaatt cgtaatgcgc cgcagttttg catattggca cgcaacagct
    54241 cgataaacgg ttcataggca cgcgcggtga taatatgcgg gtccattggc ggcaatcccc
    54301 agttctgccc caacgggccg aggatatccg gcggtgctcc aaccgacgct ttcaggcaat
    54361 acaactcccg gtcgcaccag gtttctgatc caccctctgc tacaccaacc gccagatcac
    54421 gatacaagcc aatcggcatt tcatagccct ggcttatctc ccagcaggcg gcaaactggc
    54481 tgtaagccag ccactgcaac cagagataaa aatcgacgtc atcacgatgt tcttcgcaga
    54541 actggcgcac ttctggtgaa tccacgttct gatacatctc tggccatgca ggccagcccc
    54601 agcgcatttc gtcctctttc acttgctggg catgtagcgc gtcaaacgcc gcctgccaga
    54661 acaggctatc gccttgctcg gcaacaaact gacgaaacgc ggccacctgt tcatcatcac
    54721 gttgcgcgaa acctttccac gccatgcgca atgctgtcat ttttagggcg gtaaccgtgg
    54781 agtaatcgac ccagtcggca tcccgcgtct gttgcagcgt ctgttgcgtg gtcggcaact
    54841 tccaccaggc ctgagcctct tcgctaagat ggaaatcttc gacggcgtta acgtcgatat
    54901 aaatcacatt cagccaacgg cgagaagacg ggctgtatgg gctggcactt tccggatttg
    54961 ccggatagag cgcatgaatc gggttcagac cgatgaacga cccgccacgt tttgccacat
    55021 ccaccagcat cgctttgaga tcgccaaaat ccccaatacc ccagtttttt tccgatcgca
    55081 gcgtataaag ctgaacgcag gcaccccaca gcttttgttt attcagcaac gcctgcggtt
    55141 cgtaacagcg tttcggggcg acaatcaccc tgcaatgcac gcgctggtct tcctgggtaa
    55201 acgtcagcgt gtgataacct tccggcagct tcgtcggtag attgaacgct ttgccccccg
    55261 ttacatggcc tttgtactgc gttccttctt cggtggtcag cagccagcta tattcgccgc
    55321 tgccctccac caccatcggc atttttttgc cgctggtata aaccatgaca ttcgggactg
    55381 gcgttaccgc cactttcgtg gcggtacgtt gatgcatcgc gtcaagcaaa cgccgtttgg
    55441 tttcggcgct aatcgactgc ggtttaccgt gggcattgat gtaattgggg ctaatccccg
    55501 ccgccagcgc ggcattatcc agacgtttgc tttccatcga gcttccttag cgttttgcct
    55561 gccagatacg agcctgataa tcgcgaatag agcgatccga gctaaacata ccgcagcggg
    55621 cggtattgag gatcgccgcg cgagtccagg cctcctggtc gcggtacagc acatccacct
    55681 gcttttgtgc ctctacatag gctgcgaaat ccgccatcac cagatacgga tcgccgccct
    55741 gtttgccgat actgtgcagc atctgatcga aggcatgctt atcgccgtcg ctgtatttgc
    55801 cgctttccag ctctttcaat actgcgtcca gcaccttatc tttcttccgc catttcaccg
    55861 ggtcgtagcc tttggccaga attgccttca cttgttccac ggtatgacca aaaataaaga
    55921 tattttcttc accaactttc tcggcgattt caacgttcgc accatccagc gtaccgacag
    55981 taagcgcacc attgagcgcc agtttcatat tgccggtacc ggaagcttct ttacctgccg
    56041 tcgaaatttg ttcggagata tccgccgccg ggatcagttt ttccgccgcc gaaacgcaat
    56101 aatccggcag gaacaccacc ttcaatttgt cgccaacctg cggatcgttg ttgatcacgt
    56161 cagccacttt gttgatcgca aagataatgt ttttcgccag gtagtagccc ggtgccgctt
    56221 tcgcgccaaa gaggaagacg cgcggtacgc gatcagcctg cgggttttca cgaatttctt
    56281 tgtacaacgc cagaatatgc agcagattca ggtgctggcg tttgtactca tgcagacgtt
    56341 tgatctgaat atcgaaaatc gcctgtggat tgatctcaat accggtacga actttcacaa
    56401 actctgccag acggacttta ttcgcctgct tgatctcgcg atattgctga cggaatttcg
    56461 catcatcagc gaatttttcc agattgatca gttgatcgag atcgttagtc cactcttttt
    56521 tcagtgattt atccaacaga gccgccagtg ccgggttgca ctgtttgatc cagcgacgtg
    56581 gggtaatacc gttggtgacg ttatggaatt tgttcggcca tagctggtga tattccggga
    56641 acagatcttt caccaccaga tccgagtgca gggccgcaac accgtttacc gcgaaaccgc
    56701 caaccacaca caggttcgcc atatgcactt gtttgtcgtg taccaccgcc agtttggccc
    56761 atactttttc atcgcccggc caggtttttt ccaccagcgt tttaaagcgt gtattaattt
    56821 cgttaataat ctgcatgtgg cgcggcagta agcctttcac cagtttcaca tcccagcgtt
    56881 ccagcgcttc tggcatcaag gtatggttgg tgtaagcgaa agttttactg gtgatggccc
    56941 aggcatcatc ccagctcatc tggtgctcat cgatcagcac gcgcagcagt tctggaatcg
    57001 cgatagttgg gtgggtatcg ttcagctgaa taacttcgta ctccgccagc tcgtgcagtt
    57061 tacgccccgc cagatgatgg cgacgcaaaa tatccgctac cgagcaggca cactggaagt
    57121 attgctgcat caggcgcagc tttttaccgg cagtatggtt gtcgtttgga tagagaactt
    57181 tggtcagttt ttccgcgttg atgccctgct gttcggcacg caggaaatca ccatcgttaa
    57241 atttagtcag atcaaacgga tgcgcatgcg tcgcctgcca cagacgcagc ggctgcgcca
    57301 cgccattacg atagccgaca acggggagat cccacgcttg accggtaatg gtaaactccg
    57361 gctcccagcg tccgtctttc gtcactttac cgccaatccc tacctgcaca tccagtgctt
    57421 cgttgtggcg gaaccacggg tagttactgc gatgccagtc atccggcgct tcaacctgtt
    57481 tgccatcgac aaatgactgg cggaacaagc catattgata attaaggccg taaccagtag
    57541 ctgactgccc gacagttgcc attgagtcga ggaagcacgc cgccagacgt cccagaccac
    57601 cgttacccag cgccgggtcg atctcttctt ccagcaagtc agtcaggttg atgtcataag
    57661 ccttcaacga atcctgtaca tcctgatacc agccgagatt caacagattg ttgcccgtca
    57721 ggcgaccaat caaaaactcc attgagatgt agttaacatg ccgctgattc gccaccggtt
    57781 tggcaaatgg ctgagcacgc agcatttcgg ccagtgcttc gctcactgcc aaccaccact
    57841 gacgaggggt catttcagcc gcagaattta agccataacg ctgccactga cgtgaaagcg
    57901 cttcctgaaa ttgcttatcg ttaaaaatag gttgtgacat aggagttcca cttttcttag
    57961 attttcaaca caacgttatc gctagtttgc caggctcgat gttgaccttc ctcatcctgc
    58021 gggggattag gcagggagga gttgcgggga tgagcaagga aatgtgatct cgaccactta
    58081 aagctagtgc aaaccacagg attagcagca aatcaatgcc atacagcgca gaaaatctgt
    58141 atcaaagtgc aaaaaatggc cgttgcgtat tttcaaaaag cggaaggtaa ctctataatt
    58201 aagtaaagga gtgaaacagt ttcacaagta aaatatccag tgtgctccat ctcattctta
    58261 atagatttat taagatcatc tttttagatg gcactttcat cagaaatgaa gaagaaaccc
    58321 ttgcttaaat gaaactgatg aacataagga aaataccgta caacccagta ttcacgctgg
    58381 atcagcgtcg ttttaggtga gttgttaata aacatttgga attgtgacac agtgcaaatt
    58441 cagacacata aaaaaacgtc atcgcttgca ttagaaaggt ttctggccga ccttataacc
    58501 attaattacg aagcgcaaaa aaaataatat ttcctcattt tccacagtga agtgattaac
    58561 tatgctgatt ccgtcaaaat taagtcgccc ggttcgactc gaccataccg tggttcgtga
    58621 gcgcctgctg gctaaacttt ccggcgcgaa caacttccgg ctggcgctga tcacaagtcc
    58681 tgcgggctac ggaaagacca cgctcatttc ccagtgggcg gcaggcaaaa acgatatcgg
    58741 ctggtactcg ctggatgaag gtgataacca gcaagagcgt ttcgccagct atctcattgc
    58801 cgccgtgcaa caggcaacca atggtcactg cgcgatatgt gagacgatgg cgcaaaaacg
    58861 gcaatatgcc agcctgacgt cactcttcgc ccagcttttc attgagctgg cggaatggca
    58921 tagcccactt tatctggtca tcgatgacta tcatctgatc actaatcctg tgatccacga
    58981 gtcaatgcgc ttctttattc gccatcaacc agaaaatctc acccttgtgg tgttgtcacg
    59041 caaccttccg caactgggca ttgccaatct gcgtgttcgt gaccagctgc tggaaattgg
    59101 cagtcagcaa ctggcattta cccatcagga agcgaagcag ttttttgatt gccgtctgtc
    59161 atcgccaatt gaagccgcag aaagcagtcg gatttgcgat gacgtttccg gttgggcgac
    59221 ggcactgcag ctaatcgccc tctccgcccg gcagaatact cactcagccc ataagtcggc
    59281 acgccgcctg gcgggaatca atgccagcca tctttcggat tatctggtcg atgaggtttt
    59341 ggataacgtc gatctcgcaa cgcgccattt tctgttgaaa agcgccattt tgcgctcaat
    59401 gaacgatgca ctcatcaccc gtgtgaccgg cgaagaaaac gggcaaatgc gcctcgaaga
    59461 gattgagcgt caggggctgt ttttacagcg gatggatgat accggcgatt ggttttgcta
    59521 tcacccactg tttggtaact tcctgcgcca gcgctgccag tgggaactgg cggcggagct
    59581 gccggaaatc caccgtgccg ccgcagaaag ctggatggcc cagggatttc ccagcgaagc
    59641 gattcatcat gcgctggcgg caggcgatgc gctgatgctg cgcgatattc tgcttaatca
    59701 cgcctggagt ttgttcaacc atagcgaact gtcgctgctg gaagagtcgc ttaaggccct
    59761 gccgtgggac agtttgctgg aaaatccgca gttggtgtta ttgcaggcgt ggctgatgca
    59821 aagccagcat cgctacggcg aagttaacac cctactggcc cgcgctgaac atgaaatcaa
    59881 ggacattaga gaaggcacca tgcacgcaga atttaacgct ctgcgcgccc aggtagcgat
    59941 taacgatggt aatccggatg aagcggaacg gctggcaaaa ctggcactgg aagagctgcc
    60001 gccgggctgg ttctatagcc gcattgtggc aacctcggtg ctgggtgaag tgctgcactg
    60061 caaaggcgaa ttgacccgct cactggcgct aatgcagcaa accgaacaga tggcacgcca
    60121 gcacgacgtc tggcactacg ccttatggag tttaatccag caaagtgaaa ttctgtttgc
    60181 tcagggattc ctgcaaaccg cgtgggaaac gcaggaaaaa gcgttccagc tgatcaacga
    60241 gcagcatctg gaacagctgc caatgcatga gtttctggtg cgcatccgag cacaactgtt
    60301 atgggcctgg gcacggctgg atgaagccga agcgtcggcg cgtagcggga ttgaagtctt
    60361 gtcgtcttat cagccacagc aacagcttca gtgcctggca atgttgattc aatgctcgct
    60421 ggcccgtggt gatttagata acgctcgtag ccagcttaac cgtctggaaa acctgctggg
    60481 gaatggcaaa tatcacagcg actggatctc taacgccaac aaagtccggg tgatttactg
    60541 gcaaatgacc agcgataaag ccgccgctgc caactggttg cgtcatacgg ctaaaccgga
    60601 gtttgcgaac aaccacttcc tgcaaggtca atggcacaac attgcccgtg cacaaatctt
    60661 gctgggcgag tttgaaccgg cagaaattgt tctcgaagaa ctcaatgaaa acgcccgcag
    60721 tctgcggttg atgagcgatc tcaaccgtaa cctgttgctg cttaatcaac tgtactggca
    60781 ggccggacgt aaaagtgacg cccagcgtgt gttgctggac gcattaaaac tggcgaatcg
    60841 caccggattt atcagtcatt ttgtcatcga aggcgaagcg atggcgcaac aactgcgcca
    60901 gcttattcaa ctcaatacgc tgccggagct ggaacagcat cgcgcacagc gtattctgcg
    60961 agaaatcaat caacatcatc ggcataaatt cgcccatttc gatgagaatt tcgttgaacg
    61021 tctgctaaat catccggaag tgccagaact gatccgcacc agcccgttga cccaacgtga
    61081 atggcaggtc ctggggctga tttactccgg ttacagcaac gagcaaattg caggtgagct
    61141 ggaagtggcg gcaaccacca tcaagacgca tatccgcaat ctgtaccaaa aactcggtgt
    61201 cgcccaccgt caggccgcgg tacaacacgc gcagaaactg ctgaagatga tgggatatgg
    61261 ggtgtgagtt tagccggata atacgtcgga tccggcttac atctttatat cattcaatgc
    61321 tcacccgcgt tacgccatcc gcctccacca gactaaaccg cactggcaag aaacgctcca
    61381 ccaccgcaat attggtcagc agatggcatg agggatgggc gaccgtaaat tctcccgcgc
    61441 ccgccagcgc catcggcagt accagctggt cggcgagata ttcccccacc gccgccggac
    61501 ttgccaggta gcgtttcacc tctttcacca actgtgccgc gaccacttcg gcgctgacgc
    61561 gcttttcacc gacgacaaaa aagcgttcgg tgatattttc actttcgact tcaagcgaaa
    61621 cggtattacc cggtccctgg tcacgcggca ggttatgaat attctgctca tgcagggaaa
    61681 aactccccgc cagtgtagcg atttcacgct cagcgacatg gcgcggcacg ccagctaata
    61741 gcacctctcc acgcatctgc acaatgttcc cgcgctcgcc aagttgcaag gtgttaaaca
    61801 atgccaccgg cgagacttcc gttgccacca ccccgcctcc agcaggataa aaaccgtggc
    61861 gtaataatgt ggtttgctga tgaatgccca ttttcgccag cagcggctcc agcacccggc
    61921 ggataaaatc cgcaggcggt gccgacgggt tatcggtgcc gccgctcact tcaacacgcg
    61981 aaggcccatc agcaaaccac agcgcgggta gcaccgtttg cagcaccagc gtgctgcttc
    62041 cggcactacc gatagcaaag cggtaatcgc cgccacgcac ggtaccgggc cggaagacca
    62101 gacgctgtga ccccagctcc gcgccttcca ccgttgccct acaaatttcc gcagccgctt
    62161 ttaccgcggt cagatgttgg cgcaacagcc ccggtttcgc ccgcccggca cgaatgccgg
    62221 tgatggtaaa tggctggccg gttatcatcg acaggctcag cgccgagcgc aggatctgcc
    62281 cgccgccttc gccctgtgcg ccatccagcg caatcatcct tttcatcatt tatcctttta
    62341 cgcacaccac ctgacgcagg gtatagataa cttccaccag atcgctttgt gccgccatca
    62401 ccgcatcaat atctttatac gccatcggga tttcgtcgat cacttcggca tctttacggc
    62461 attccacatg cgcggtggca cgaatttgat cttccacgct gaacattttt ttcgctttag
    62521 tacggctcat aacccgcccg gcaccgtggc tgcacgaaca gaacgactct tcatttccca
    62581 gcccacggac gataaagctt ttcgctccca tcgaccctgg aataattcca tattgaccag
    62641 cacgcgcaga caccgcgcct ttacgcgtca cgtagatctc ttcaccaaag tgctgttctt
    62701 tttgcacata gttgtggtga cagttgatct cttccatcgc cagcgtttgt ggctgtctga
    62761 ccgttttctg cgtaatgctc tgcaacgccg ttaccacgtt ttccatcatc gcatcgcggt
    62821 taaggctggc aaaaagctgc gcccaggcca cggctttcag gtaatcatca aagtattccg
    62881 taccttccat aaagtacgcc agatcacgcg acggcaatgt ttcaagcgtt tcctgcatct
    62941 ctttttgcgc cagatcgata aagtaagtcc cgatggcgtt accaattccg cgtgaaccgg
    63001 agtgcagcat aatccacacc tggtccgact catcaaggca gatttcaata aagtggttac
    63061 ccgttcccag cgttcccagg tgtttatagt tgttagtgtt aaggaaacgg ggatattttt
    63121 gcgttaacca ctgataaccg gcttccagct cagcccattt cgcatcgacg ttaacaggcg
    63181 gattttccca tgcgccttta tcacgtttac aacgtccggt agtacgtccg tgcggcacgg
    63241 ctgtttcaat cgcctgacgc agctccgcca ggttttccgg caggtctgcc gccgttaacg
    63301 cggtacgcag cgcgttcatt ccacaaccga tatccacgcc caccgccgcc ggaataatcg
    63361 cccctttggt cgggatcacg ctaccaatgg tggaaccttt acccaggtgt acatccggca
    63421 ttaccgcaat atgtttgaaa ataaacggca tcttcgccgt attaataagt tgctgacgcg
    63481 catcggcctc taccggcacg cctttggtcc acatttttac cggggcattt tcagtggtca
    63541 gtaattcgta attcattttg tttttctctt ttcgttgttt gctgtcctga taattgcaac
    63601 cgtcgtgcca gaaaattaaa taagcagctt aattttttta tttattgttt ttaaaaagat
    63661 tatatcttta cgtccgtaac cggagatttc ccgcaaagcc gatttaccga taatgaaatc
    63721 tcgtctttta taaggatatc taagatgcgt aaaacagtgg cttttggctt tgtcggaacg
    63781 gtgctggatt atgccgggcg cggcagtcag cgctggtcga aatggcgtcc gtcactctgt
    63841 ctatgccagc aagaatcgtt agtcatcaat cgactggaat tgttgcacga cgcccgctcg
    63901 cgctcgctgt ttgaaacgct taaatgcgat atcgccagcg tttcgccaga aactgaagtg
    63961 gtgagcgttg agattgaact gcataacccg tgggatttcg aagaggttta cgcctgcctg
    64021 catgatttcg cccgtggcta cgcgtttcaa cctgataaag aagactattt aattcacatc
    64081 accactggca cccacgtcgc acagatttgc tggtttctgc tggcggaagc gcgttacctg
    64141 cccgcccggc tgatacaatc ttccccaccg cgcaaaaaag aacagccgcg cggcgcaggt
    64201 gaagtgacga ttatcgatct cgatttaagc cgttataacg ccatcgccag ccgctttgcc
    64261 gaggaacggc agcaaacgct cgattttctt aagtccggca ttgccacgcg taacccccac
    64321 ttcaaccgca tgattgagca gattgaaaaa gtggcgatta aatcccgcgc accgatcctg
    64381 cttaacggcc cgacaggtgc gggcaagtca tttctggcac gacgcatctt cgaattaaaa
    64441 caggcacggc atcagtttag cggcgcattt gtagaaatga actgcgccac tctgcgtggc
    64501 gataccgcca tgtcgacgct atttggtcat gtgaaaggcg cgtttaccgg ggcgcgggag
    64561 tcgcgggaag gattattacg cagcgccaac ggcgggatgt tatttcttga tgagattggc
    64621 gaactgggcg cagacgaaca ggcaatgctg ctgaaagcca ttgaagagaa aaccttttat
    64681 ccgtttggca gcgatcgcca ggtgagtagc gactttcagc ttatcgccgg aacggtgcgc
    64741 gatttgcgtc agctggttgc cgaaggcaaa ttccgcgaag atctgtacgc gcggatcaat
    64801 ctctggacct tcaccttgcc ggggctgagc cagcgccagg aagatattga accgaacctt
    64861 gattatgaag tggagcgcca cgcctcactc actggcgaca gcgtgcgttt taacaccgaa
    64921 gcgcggcgcg cctggctggc ttttgcgacc tctccccagg caacatggcg cggtaacttt
    64981 cgcgaacttt ctgccagcgt cacgcggatg gcaacctttg ccactagcgg acgcatcact
    65041 cgggaagtgg tggaagatga gataaaccgt ctgcgctata actggcagga gagtcgcccc
    65101 tccgcgatta cggcgttgct gggcgcagag gcagaaaaca tcgatctctt cgaccgtatg
    65161 caactggaac acgtaatagc catctgccgc caggcaaagt cactttctgc tgccggtcgc
    65221 cagctttttg atgtttcgcg ccagggcaaa gccagcgtca acgacgcaga tcggctacgc
    65281 aaatacctgg cgcgttttgg tctgacgtgg gaagccgtgc aggatcagca cagctccagt
    65341 tgaatatgat ggtccgtcag cacctgcatg acgcttactg gcggcggggc gtcggtgtag
    65401 acagcatcaa ccatgctgat gctccccata ttgaccatcg cattacggcc aaatttcgag
    65461 tgatcgacaa ccagcataac gtggcgcgag ttctcaataa tggcgcgttt ggtgcgaact
    65521 tcgtggtaat cgaattccag caacgagccg tcgctatcga tgccgctgat ccccagaatg
    65581 ccgaaatcaa ggcggaactg ggagataaaa tcgagcgtcg cttcgcctat gatcccacca
    65641 tcgcggctac gtaattcgcc accggcgaga ataatgcgaa aatcctcttt taccatcagc
    65701 gtgttagcga cgttaagatt gttggtgaca atgcgcaaat tgctgtgatt gagcagcgcg
    65761 tgcgccaccg cttccggggt ggtgccgata tcgataaaca gcgttgagcc attaggaatt
    65821 tgctccgcca ctttgcgggc gatgcgctct ttttcttcgg tctgggtggc tttacgatcg
    65881 tgccatggcg tgttaaccga actggaaggc agcgccgcac cgccatgatg gcgcaggatc
    65941 aggttttgct ctgccagctc gttgaggtcg cgacgaatag tctgcgggct gacggagaag
    66001 tgctccacca gttcctcggt actgacataa ccctgctgtt taaccagttc gataataccg
    66061 ttgtgacgtt gtgtttgttt catttataaa tccctggaat tattttcgtt ttcgcgcatt
    66121 gagcgaatca acaaaagcca tcgctaaacc cacggctaac ccggcgatgt gtgctccgtt
    66181 cgccatcgac atcccaaaca aatcaaacca tccggcgaca atccagatca gcgcaaagat
    66241 aattaaccca cgttgcaggt aaatgccact ttgcggatcg cgttcgccac gtagccagac
    66301 gtagcccatc agcgcataca ccacgccaga aagcccgcca aaccacggcc cgctgaattt
    66361 ttgctgcaca tagccgctta acagggcgct aatgagagta atgacaatta gcttaccgct
    66421 accgaggcgt ttttccaccg caccgccgag ataccaccac cagagcaggt taaagaggat
    66481 atgcatcagc gagaagtgca ttaacgcgtg ggtgaagtaa cgccagaact caaatttcag
    66541 tgttggatcg aatggccagg ccagccataa catcacttcc tgatcgccga gaatttgcat
    66601 ggcaataaac accaccacgc aggcgatcat catcacccag gttaccggac ctgcgcgttc
    66661 acgcaaggca gcaaagaaag gataacggcg ataatgcagg ccactgccgg tatggcccgc
    66721 ctgccagctg gccgccagat aacgcggatc tgccgggttt tcgagaaaac gcgccagctc
    66781 tgcccgcacg cgctcggcct gggactcatc cgccagccag acatcgcttt ggttatgttg
    66841 ttgaatcgtg aggataacac cctgcgtcgc catgtaatca acaaacgcct gcgccacgcg
    66901 ggggttagca aaagaggtaa tcatcaacat cgttgctgtc gcttattcca cacaaaaggg
    66961 gacagtataa agcgttacgc gccgtacgcc acctctgcag gaaactgacg ccgccaggct
    67021 tcaaagccgc cgtcaatgct atagaccaca tcgtagccct gttgcagcag atactgcgcc
    67081 gcgcctttgc tgctattgcc gtgataacac atcaccatca ccggtgtgtc aaagtcgtta
    67141 tcacgcataa acgcgcccag tgtgtcgtta gttaaatgga aagcctgcac cgcatgcccc
    67201 attgcgaaac tctgtggatc gcgaatatcg accagcaccg cctctttttc ctgcaacttc
    67261 tggtgcgcga cggcaacgtt aatacattcg aactgctcca tgcgtctctc tttctttaca
    67321 aacaagtggg caaatttacc gcacagttta cgtcgaagcg gcagataaac gccataatgt
    67381 tatacatatc actctaaaat gttttttcaa tgttacctaa agcgcgattc tttgctaata
    67441 tgttcgataa cgaacattta tgagctttaa cgaaagtgaa tgagggcagc atggaaacca
    67501 aagatctgat tgtgataggt ggcggcatca atggtgctgg tatcgcggca gacgccgctg
    67561 gacgcggttt atccgtgctg atgctggagg cgcaggatct cgcttgcgcg acctcttccg
    67621 ccagttcaaa actcattcac ggtggcctgc gctaccttga gcactatgaa ttccgcctgg
    67681 tcagcgaggc gctggctgaa cgtgaagtgc tactgaaaat ggccccgcat atcgccttcc
    67741 cgatgcgttt tcgcctgccg catcgtccgc atctgcgccc ggcgtggatg attcgcattg
    67801 gtctgtttat gtacgatcat ctgggtaaac gcaccagcct gccgggatca accggtttgc
    67861 gttttggcgc aaattcagtg ttaaaaccgg aaattaagcg cggattcgaa tattccgact
    67921 gttgggtaga cgacgcccgt ctggtactcg ccaacgccca gatggtagtg cgtaaaggcg
    67981 gtgaagtgct tactcgtact cgtgccactt ctgctcgccg cgaaaacggc ctgtggattg
    68041 tggaagcgga agatatcgat accggcaaaa aatatagctg gcaagcgcgc ggcctcgtga
    68101 acgccaccgg cccgtgggta aagcagttct tcgacgacgg gatgcatctg ccttcgcctt
    68161 atggcattcg cctgatcaaa ggcagccata ttgtcgtgcc gcgcgttcat acacaaaagc
    68221 aggcctacat tctgcaaaac gaagataaac gtattgtctt cgtgatcccg tggatggacg
    68281 agttttccat catcggcacc accgatgtgg aatacaaagg tgatccaaaa gcagtgaaga
    68341 ttgaagagag tgaaatcaat tacctgctga aagtgtataa cacgcacttt aaaaagcagt
    68401 taagccggga cgatatcgtc tggacttact ccggcgtgcg tccgctgtgt gatgatgagt
    68461 ccgattcgcc gcaggctatc actcgtgatt acacccttga tatccatgat gaaaacggca
    68521 aagcgccgct gctgtcggta ttcggcggta agctgaccac ctaccgcaaa ctggcggagc
    68581 acgcgctgga aaaactgacg ccatattatc agggcattgg tccggcgtgg acgaaagaga
    68641 gcgtgctgcc gggtggtgcc attgaaggcg accgcgacga ttatgccgct cgcctgcgcc
    68701 gccgctatcc gttcctgact gaatcgctgg cacgccatta cgcgcgcacg tacggcagca
    68761 acagcgagtt gctgctcggc aatgcgggga cggtaagcga tctcggggaa gatttcggtc
    68821 atgagttcta cgaagcggag ctgaaatacc tggtggatca cgaatgggtc cgccgcgccg
    68881 acgatgccct gtggcgtcgc acaaaacaag gcatgtggct gaatgcggaa caacaatctc
    68941 gtgtgagtca gtggctggtg gagtatacgc agcagaagtt gtcgctggcg tcgtaaggat
    69001 gatgcccggt tgtgaagtgc aaccgggcta tttggattcc ttagactatt cattgtaaat
    69061 aattgaataa cggcccctga gctaaaatcg ccaaacttaa gcacgttccc cactcaaccc
    69121 acttctgccc ctggcttcta tttttgctac gccactggca gtaatatccc aacgcaaaac
    69181 caagctgtgc actaatcggg ccgacaaaat cagtccacgg ctgcggccaa aaaggggaaa
    69241 aattcagggg aagccagaca aacgtcagaa agaagtttcg gatattttcc agcaacgctg
    69301 atttctctgg agtaatataa agaatgaaat tcgagagaat taaccagaaa taggccggag
    69361 aaaagaccag ccagtaatgc agtctgtttt ttaattcagg aggcgttctg cgggagaatt
    69421 gccaaccgat gccacccatt acactgaaaa aaagtgcata ttcaaccatt aactcaagtt
    69481 gtaattcctg aagagaaata cgcacaacca ggagcggaaa ggagaatatt atttcagcca
    69541 gaaaagttaa cgtgaatata agtacggcta aggcaataac cgtcggccat accaataatg
    69601 gcacgataac aaaaccgaga attacagggg gaatataaag ccggaattta atcattttta
    69661 ttttcctggt gattcggatg atgcgtcata catcgcgcat aaacactcat caatcatcca
    69721 gtcgtcttcc cagacctgat aatgtgtgcc ctgttcgggc tgattaagca ggttctcaat
    69781 cgcctgccgc gcacgttctt tcaggatgaa ttgattgttg aaggcccagg cattgatttg
    69841 ggcggtaatg gctttatcgt catcatcctg ctcaaaacga ctattctgaa tattctcttc
    69901 cctgttatgc cacagcaaac atgccatcga tactgtgggt tggtaaccgg gtacagagat
    69961 gatggcatca agcaatggcg aaagagcgat atcggcacgg gaaaacattt ccagcatata
    70021 ctgtaattcg acagcctcat cgccagatac ccaatcagcc atatactggc aggcaaatcg
    70081 ctgcaatatt gcccgttcat cctcacgcca gttttcttta ctggcaaggt tgatacgccg
    70141 tagagaaaat acttcgtgaa tattgtcaaa ttgcccctgc gcaacaaact ccagtaaacg
    70201 aggaagaaaa taacgaacct catcgctgtt ttgcggatca ggtcctggac tactattcca
    70261 ggcattgatt aaggaataag gaatcgctcg caacgatgta ttccgtaatg ctttttgttc
    70321 ctgttgactc aggcaatatt cacaacaaac cacaaattgc tgcggcaccg gataacgacg
    70381 aaaaatatga taaacctgct caattaatgc ctgcatggcg gagctgggtt gcataattac
    70441 cccctcttcc gctggtcgcg tgtaaacggt tgataaacaa aacgagcatt cgccgcattc
    70501 aggcgaataa tggaactacg agagagggaa aagctgggcc gaatgcaata attacaatcc
    70561 cgacagcttt cctctgtgca attttccact gagtaatgac gcgcgtagag tttcagttca
    70621 gcctgcttat cccgaaaacc gcgtaagctg ttatcggtat ggaatttcca tgcttcttta
    70681 aacacctgaa agatataaac atcctcaatc caggagtcac attttttaag cgggcgcttt
    70741 gctatgatca cccgctcatc cggattgagc attgccgtaa aatgaacggt aaagtcattt
    70801 gtcgtattca ggtcacattg aacatctggt acatcactga tataggggtc aaatccaaaa
    70861 tcagcaccac aggcacaaag acgaccgatc gtggcggtat cgagggaaaa tcccggccat
    70921 acattgctgg aggttaagcc aatatttaat gtgacatcat atcgagataa tccctcaaga
    70981 ccgacagtac cggcgaactt ttccccttca tccaggacct ggttaatgag ttgctgaata
    71041 atgccttcat cccagtatgt tgatttgcag ttggcaatcg taatatccac gccagtgaat
    71101 ggatcagtac cggagaggct tgcaaataac gtatcaacta attccatttt tattatccgg
    71161 cttaatgcaa cattttatcc atcggcacat aacctacatt ttccaccaga ttttgtccct
    71221 gtagaattaa aaatattttt ttgttttaca ttctgttcat ccctattatt gcattttatt
    71281 ttgcggtgat ggctaaaccg attctgtact agctcaaaaa ttactgatat tccactcgca
    71341 atacagccag tgcattataa cgacctgccg caggcacttt tccagttgta ctaacaggta
    71401 aagccaggaa agtaacatca gcagtttgcg cagaagcagc ctggttttcc accaatctga
    71461 acggaacgcc tactgatgtt gccagagcat ttaaatctac ggagttttgc tgactatcca
    71521 taatcataat accaacatca ggattatctt gtgcctgaat aatactttgt ttcccattgg
    71581 tatcacgtgt agctgaaatg ttaccgtcga ataccaacgt cattaccgca tctgtatctt
    71641 tattcgtaca attaatcgga atagttgttg ctacaggagt aacaccagca ggcttcgctc
    71701 cagcaccagc ccggctcaat tcactggccc acacatccgg catattaaca gtaaaactag
    71761 taccctcatt aatagtgcaa ccttgcggaa cctgaatatc caggttactg atccaggcat
    71821 taaataatgg tactggccca tgttccgttt gccgctgact ggcatacaat gaaactaaaa
    71881 gtacattatt cagagagaca tcagagatga tttttttatc caacctaatt ttaatatagc
    71941 cttctgttcc tgaagaccag taatctgctt tacttctgtc agcaggataa ttagtactat
    72001 agttctcgaa tggtactgtt ttatctgtca tcgtcccatt ttgattgtag agttttatat
    72061 gtgttgaaat agatatttca tgcgtaacag gaataaatat ttctgctccc tgtggattac
    72121 ctgaagcacc tgaaggatct attcctgaaa tacctgaaaa ataaacttca taatcatcaa
    72181 gagaattaca gtatgcttta tatcgaccta ttttccagtt ttgagatggt aaaacagtac
    72241 cggctgaatt gttgttaaca tctgaaataa taactgtgcc aaaatcataa aaaaaggtcg
    72301 ctggcgtagt attgtttcct ggagtaccag gatagcaaaa ctcacgatcc cggcccattt
    72361 cagcataggc tacgacatgg taccctaaga ataaaaaaat tataaaaata attttcactt
    72421 tatatccggc ataagtaatc atacgatttc cttttactaa tccgttttaa attgtgagcc
    72481 tgacacatcc atttgcaacg gattcatatt tactttccat acaggcaata cattattata
    72541 aatgtccaat gtctgccatg ttatttcaca aatcccttgc gctttacacc atgattgttg
    72601 acgatggcta aatggtgcga tcataccacc atccataact ttaccatcaa catgaaggtt
    72661 atagagtgtc acatatctgg ggcctggatt ataaacggta acaatttcat tttttcgtgc
    72721 ccagcgtaat ttttgatatg aaatattcgg gtcttcagac aaatttgatg gccgatagaa
    72781 tattttcata cgattgcgga ccgctatttg caattgatta tcagttttgg ttggatgact
    72841 attttccaac gatggtaatg cagcaacatt aagtataaat aaactttccc ggtcggcagg
    72901 taaactgcca ccaacacgat taattcttat ttttgtattt ttgtcaggct tcaaaacaaa
    72961 cagtggcgga gttgcgataa ataccgttcc agaaaatgag ggcaaagatt caaccgttcc
    73021 cgtcaactga cgagcatcat caggggagac tgcaaccttg actaacatat ctctgtctga
    73081 agtgtttttg agttcaacag atattgagga ctgattttcc ggatacgcaa aacgcgttcc
    73141 accgatcact aatcctgctt gtgctgtaga gaataaaagg gaaaataata ctatgactac
    73201 cttaatcata ggtgacaata aacgatactg acgcattggc atcccctggt tggacattat
    73261 tttttaatgc tttgtaagcc gcaaaaaaat gcaatatatt atcaactgat gatgtaagtg
    73321 aaaaacctat gcttttaaca tcattactaa tactgccttt gttcttatca aaaatcgtga
    73381 gtgcaagccc atctaccgag ccatcttcca gcttatataa actggcatct tcagaaactg
    73441 tattgccggt aaactttata tacgcgttgc caacatcact accacagcgg gttaacgtta
    73501 tggaaaaata aaccttttca gtaacatccc ctgcactcct taccgaacta ctgggtacgt
    73561 ctggcatatt aactgtaaaa ttttttgact catcagaaat aatgcaggtt ttttcggtaa
    73621 ttagcccttc aatacgaacc actccggcat gagtgatatc atcagcaaaa gaatttgttg
    73681 atattataaa caaaaataaa ctaatgaata tcttcatatc tccctcactg ataatcgata
    73741 tcaaaataca acagagcatt tgcttgcccg gcatgtacat tttcctgagt tgatttatag
    73801 cgaagtttaa acgtcaaatt agcatcaccg ttttcattta acactaaatc acgaaaacct
    73861 gattcaccat taatagcaat cgccacatca ttgttatcca atatttctat tcccagtcca
    73921 gtcgcactat ctgcaccggc ggagagacgc agtaatgcgc tattaatggc atctgcatca
    73981 ccagtaaacc gcacattggc gctcacacca gaactataaa cagtactttt tgcagtcggg
    74041 catccttgta cattaatcgt aaacgtttga ctcggagaaa catcacctac atttaaaaat
    74101 tcattagcat cataagtgcc catttttacc gactgatctt tacttccggt agtaatgtca
    74161 cagctttttg ccaacaaaat cccagtaaat gtaacaacaa ctgaatca
//
LOCUS       DAZGHF010000006        69084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGHF010000006 DAZGHF010000000
VERSION     DAZGHF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.006"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(13..89)
                     /locus_tag="ACHK3A_000462"
     tRNA            complement(13..89)
                     /locus_tag="ACHK3A_000462"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53..55),aa:Arg,seq:acg)
     gene            complement(288..364)
                     /locus_tag="ACHK3A_000463"
     tRNA            complement(288..364)
                     /locus_tag="ACHK3A_000463"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(328..330),aa:Arg,seq:acg)
     gene            complement(368..460)
                     /locus_tag="ACHK3A_000464"
     tRNA            complement(368..460)
                     /locus_tag="ACHK3A_000464"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Ser,seq:gct)
     gene            complement(776..961)
                     /gene="csrA"
                     /locus_tag="ACHK3A_000465"
     CDS             complement(776..961)
                     /gene="csrA"
                     /locus_tag="ACHK3A_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO8738603.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1196..3826)
                     /gene="alaS"
                     /locus_tag="ACHK3A_000466"
     CDS             complement(1196..3826)
                     /gene="alaS"
                     /locus_tag="ACHK3A_000466"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO8738604.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVNSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLNEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3954..4454)
                     /gene="recX"
                     /locus_tag="ACHK3A_000467"
     CDS             complement(3954..4454)
                     /gene="recX"
                     /locus_tag="ACHK3A_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO8738605.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4523..5584)
                     /gene="recA"
                     /locus_tag="ACHK3A_000468"
     CDS             complement(4523..5584)
                     /gene="recA"
                     /locus_tag="ACHK3A_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO8738606.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5664..6161)
                     /gene="pncC"
                     /locus_tag="ACHK3A_000469"
     CDS             complement(5664..6161)
                     /gene="pncC"
                     /locus_tag="ACHK3A_000469"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO8738607.1"
                     /translation="MTDSELMQLSEQVGQSLKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6306..7391)
                     /gene="mltB"
                     /locus_tag="ACHK3A_000470"
     CDS             complement(6306..7391)
                     /gene="mltB"
                     /locus_tag="ACHK3A_000470"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO8738608.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7647..8210
                     /gene="srlA"
                     /locus_tag="ACHK3A_000471"
                     /gene_synonym="gutA"
     CDS             7647..8210
                     /gene="srlA"
                     /locus_tag="ACHK3A_000471"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO8738609.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8207..9166
                     /gene="srlE"
                     /locus_tag="ACHK3A_000472"
                     /gene_synonym="gutE"
     CDS             8207..9166
                     /gene="srlE"
                     /locus_tag="ACHK3A_000472"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO8738610.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9177..9548
                     /gene="srlB"
                     /locus_tag="ACHK3A_000473"
                     /gene_synonym="gutB"
     CDS             9177..9548
                     /gene="srlB"
                     /locus_tag="ACHK3A_000473"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO8738611.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9552..10331
                     /gene="srlD"
                     /locus_tag="ACHK3A_000474"
     CDS             9552..10331
                     /gene="srlD"
                     /locus_tag="ACHK3A_000474"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO8738612.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10437..10796
                     /gene="gutM"
                     /locus_tag="ACHK3A_000475"
     CDS             10437..10796
                     /gene="gutM"
                     /locus_tag="ACHK3A_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO8738613.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMYAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10863..11636
                     /gene="srlR"
                     /locus_tag="ACHK3A_000476"
                     /gene_synonym="gutR"
     CDS             10863..11636
                     /gene="srlR"
                     /locus_tag="ACHK3A_000476"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO8738614.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            11629..12594
                     /gene="gutQ"
                     /locus_tag="ACHK3A_000477"
     CDS             11629..12594
                     /gene="gutQ"
                     /locus_tag="ACHK3A_000477"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO8738615.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12591..14105)
                     /gene="norR"
                     /locus_tag="ACHK3A_000478"
     CDS             complement(12591..14105)
                     /gene="norR"
                     /locus_tag="ACHK3A_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO8738616.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     IVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14292..15731
                     /gene="norV"
                     /locus_tag="ACHK3A_000479"
     CDS             14292..15731
                     /gene="norV"
                     /locus_tag="ACHK3A_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO8738617.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLTDIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15728..16861
                     /gene="norW"
                     /locus_tag="ACHK3A_000480"
     CDS             15728..16861
                     /gene="norW"
                     /locus_tag="ACHK3A_000480"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO8738618.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQRQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRNARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILAALMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(17085..19337)
                     /gene="hypF"
                     /locus_tag="ACHK3A_000481"
     CDS             complement(17085..19337)
                     /gene="hypF"
                     /locus_tag="ACHK3A_000481"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO8738619.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAGLGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19490..20017)
                     /gene="hydN"
                     /locus_tag="ACHK3A_000482"
     CDS             complement(19490..20017)
                     /gene="hydN"
                     /locus_tag="ACHK3A_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO8738620.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20166..21176)
                     /gene="ascG"
                     /locus_tag="ACHK3A_000483"
     CDS             complement(20166..21176)
                     /gene="ascG"
                     /locus_tag="ACHK3A_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO8738621.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21436..22893
                     /gene="ascF"
                     /locus_tag="ACHK3A_000484"
     CDS             21436..22893
                     /gene="ascF"
                     /locus_tag="ACHK3A_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO8738622.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            22902..24326
                     /gene="ascB"
                     /locus_tag="ACHK3A_000485"
     CDS             22902..24326
                     /gene="ascB"
                     /locus_tag="ACHK3A_000485"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO8738623.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LARTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24484..24954)
                     /gene="hycI"
                     /locus_tag="ACHK3A_000486"
     CDS             complement(24484..24954)
                     /gene="hycI"
                     /locus_tag="ACHK3A_000486"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO8738624.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(24947..25357)
                     /gene="hycH"
                     /locus_tag="ACHK3A_000487"
     CDS             complement(24947..25357)
                     /gene="hycH"
                     /locus_tag="ACHK3A_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO8738625.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25354..26121)
                     /gene="hycG"
                     /locus_tag="ACHK3A_000488"
     CDS             complement(25354..26121)
                     /gene="hycG"
                     /locus_tag="ACHK3A_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO8738626.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(26121..26663)
                     /gene="hycF"
                     /locus_tag="ACHK3A_000489"
     CDS             complement(26121..26663)
                     /gene="hycF"
                     /locus_tag="ACHK3A_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO8738627.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(26673..28382)
                     /gene="hycE"
                     /locus_tag="ACHK3A_000490"
     CDS             complement(26673..28382)
                     /gene="hycE"
                     /locus_tag="ACHK3A_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO8738628.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28400..29323)
                     /gene="hycD"
                     /locus_tag="ACHK3A_000491"
     CDS             complement(28400..29323)
                     /gene="hycD"
                     /locus_tag="ACHK3A_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO8738629.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29326..31152)
                     /gene="hycC"
                     /locus_tag="ACHK3A_000492"
     CDS             complement(29326..31152)
                     /gene="hycC"
                     /locus_tag="ACHK3A_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO8738630.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(31149..31760)
                     /gene="hycB"
                     /locus_tag="ACHK3A_000493"
     CDS             complement(31149..31760)
                     /gene="hycB"
                     /locus_tag="ACHK3A_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO8738631.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(31885..32346)
                     /gene="hycA"
                     /locus_tag="ACHK3A_000494"
     CDS             complement(31885..32346)
                     /gene="hycA"
                     /locus_tag="ACHK3A_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO8738632.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32558..32908
                     /gene="hypA"
                     /locus_tag="ACHK3A_000495"
     CDS             32558..32908
                     /gene="hypA"
                     /locus_tag="ACHK3A_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO8738633.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            32912..33784
                     /gene="hypB"
                     /locus_tag="ACHK3A_000496"
     CDS             32912..33784
                     /gene="hypB"
                     /locus_tag="ACHK3A_000496"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO8738634.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            33775..34047
                     /gene="hypC"
                     /locus_tag="ACHK3A_000497"
     CDS             33775..34047
                     /gene="hypC"
                     /locus_tag="ACHK3A_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO8738635.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            34047..35168
                     /gene="hypD"
                     /locus_tag="ACHK3A_000498"
     CDS             34047..35168
                     /gene="hypD"
                     /locus_tag="ACHK3A_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO8738636.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAETHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35165..36175
                     /gene="hypE"
                     /locus_tag="ACHK3A_000499"
     CDS             35165..36175
                     /gene="hypE"
                     /locus_tag="ACHK3A_000499"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO8738637.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36249..38327
                     /gene="flhA"
                     /locus_tag="ACHK3A_000500"
     CDS             36249..38327
                     /gene="flhA"
                     /locus_tag="ACHK3A_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO8738638.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRHDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLSLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            38372..38800
                     /locus_tag="ACHK3A_000501"
     CDS             38372..38800
                     /locus_tag="ACHK3A_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO8738639.1"
                     /translation="MAVSARNQLTGTVSTVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(38839..39183)
                     /gene="ygbA"
                     /locus_tag="ACHK3A_000502"
     CDS             complement(38839..39183)
                     /gene="ygbA"
                     /locus_tag="ACHK3A_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO8738640.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            39469..42030
                     /gene="mutS"
                     /locus_tag="ACHK3A_000503"
     CDS             39469..42030
                     /gene="mutS"
                     /locus_tag="ACHK3A_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO8738641.1"
                     /translation="MRTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLEGVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIATGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42136..42792
                     /gene="pphB"
                     /locus_tag="ACHK3A_000504"
     CDS             42136..42792
                     /gene="pphB"
                     /locus_tag="ACHK3A_000504"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO8738642.1"
                     /translation="MPSTRYQKINAHHYHHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(42843..43610)
                     /gene="ygbI"
                     /locus_tag="ACHK3A_000505"
     CDS             complement(42843..43610)
                     /gene="ygbI"
                     /locus_tag="ACHK3A_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO8738643.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            43806..44714
                     /gene="ltnD"
                     /locus_tag="ACHK3A_000506"
     CDS             43806..44714
                     /gene="ltnD"
                     /locus_tag="ACHK3A_000506"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO8738644.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            44711..45973
                     /gene="otnK"
                     /locus_tag="ACHK3A_000507"
     CDS             44711..45973
                     /gene="otnK"
                     /locus_tag="ACHK3A_000507"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO8738645.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSSAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDTPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            45970..46608
                     /gene="ygbL"
                     /locus_tag="ACHK3A_000508"
     CDS             45970..46608
                     /gene="ygbL"
                     /locus_tag="ACHK3A_000508"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO8738646.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            46613..47389
                     /gene="ygbM"
                     /locus_tag="ACHK3A_000509"
     CDS             46613..47389
                     /gene="ygbM"
                     /locus_tag="ACHK3A_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO8738647.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLAQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADHFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            47478..48842
                     /gene="ygbN"
                     /locus_tag="ACHK3A_000510"
     CDS             47478..48842
                     /gene="ygbN"
                     /locus_tag="ACHK3A_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO8738648.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCI
                     WAVI"
     gene            complement(48880..49116)
                     /locus_tag="ACHK3A_000511"
     CDS             complement(48880..49116)
                     /locus_tag="ACHK3A_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO8738649.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWMVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(49127..50554)
                     /locus_tag="ACHK3A_000512"
     CDS             complement(49127..50554)
                     /locus_tag="ACHK3A_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO8738650.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFNILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARQ"
     gene            complement(50554..51147)
                     /locus_tag="ACHK3A_000513"
     CDS             complement(50554..51147)
                     /locus_tag="ACHK3A_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO8738651.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            51294..51701
                     /locus_tag="ACHK3A_000514"
     CDS             51294..51701
                     /locus_tag="ACHK3A_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO8738652.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEQELFMQLVRKMMSK"
     gene            complement(51822..52814)
                     /gene="rpoS"
                     /locus_tag="ACHK3A_000515"
     CDS             complement(51822..52814)
                     /gene="rpoS"
                     /locus_tag="ACHK3A_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO8738653.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(52877..53971)
                     /gene="nlpD"
                     /locus_tag="ACHK3A_000516"
     CDS             complement(52877..53971)
                     /gene="nlpD"
                     /locus_tag="ACHK3A_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO8738654.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(54156..54782)
                     /gene="pcm"
                     /locus_tag="ACHK3A_000517"
     CDS             complement(54156..54782)
                     /gene="pcm"
                     /locus_tag="ACHK3A_000517"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO8738655.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(54776..55537)
                     /gene="surE"
                     /locus_tag="ACHK3A_000518"
     CDS             complement(54776..55537)
                     /gene="surE"
                     /locus_tag="ACHK3A_000518"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO8738656.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(55518..56567)
                     /gene="truD"
                     /locus_tag="ACHK3A_000519"
     CDS             complement(55518..56567)
                     /gene="truD"
                     /locus_tag="ACHK3A_000519"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO8738657.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQKLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(56564..57043)
                     /gene="ispF"
                     /locus_tag="ACHK3A_000520"
     CDS             complement(56564..57043)
                     /gene="ispF"
                     /locus_tag="ACHK3A_000520"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO8738658.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(57043..57753)
                     /gene="ispD"
                     /locus_tag="ACHK3A_000521"
     CDS             complement(57043..57753)
                     /gene="ispD"
                     /locus_tag="ACHK3A_000521"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO8738659.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(57772..58083)
                     /gene="ftsB"
                     /locus_tag="ACHK3A_000522"
     CDS             complement(57772..58083)
                     /gene="ftsB"
                     /locus_tag="ACHK3A_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO8738660.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(58276..58599)
                     /gene="ygbE"
                     /locus_tag="ACHK3A_000523"
     CDS             complement(58276..58599)
                     /gene="ygbE"
                     /locus_tag="ACHK3A_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO8738661.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(58649..59254)
                     /gene="cysC"
                     /locus_tag="ACHK3A_000524"
     CDS             complement(58649..59254)
                     /gene="cysC"
                     /locus_tag="ACHK3A_000524"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO8738662.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(59254..60681)
                     /gene="cysN"
                     /locus_tag="ACHK3A_000525"
     CDS             complement(59254..60681)
                     /gene="cysN"
                     /locus_tag="ACHK3A_000525"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO8738663.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(60683..61591)
                     /gene="cysD"
                     /locus_tag="ACHK3A_000526"
     CDS             complement(60683..61591)
                     /gene="cysD"
                     /locus_tag="ACHK3A_000526"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO8738664.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            61843..62880
                     /gene="iap"
                     /locus_tag="ACHK3A_000527"
     CDS             61843..62880
                     /gene="iap"
                     /locus_tag="ACHK3A_000527"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO8738665.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   62962..63295
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=63023..63049
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaac"
     gene            complement(63401..63685)
                     /gene="cas2e"
                     /locus_tag="ACHK3A_000528"
     CDS             complement(63401..63685)
                     /gene="cas2e"
                     /locus_tag="ACHK3A_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO8738666.1"
                     /translation="MSMLVVVTENVPSRLRGRLAIWLLEVRAGVYVGDVSARVREMIW
                     EQISGLAEEGNVVMAWATNTESGFEFQTFGVNRRTPVDLDGLRLVSFLPV"
     gene            complement(63687..64604)
                     /gene="cas1e"
                     /locus_tag="ACHK3A_000529"
     CDS             complement(63687..64604)
                     /gene="cas1e"
                     /locus_tag="ACHK3A_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO8738667.1"
                     /translation="MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYVLLAKQYGVKW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYD
                     IADIIKFDTVVPKAFEIARRIPGEPDREVRLACRDIFRSSKTLVKLIPLIEDVLAAGE
                     IQPPAPPEDAQPVAIPLPVSLGDAGHRSS"
     gene            complement(64620..65219)
                     /gene="cas6e"
                     /locus_tag="ACHK3A_000530"
     CDS             complement(64620..65219)
                     /gene="cas6e"
                     /locus_tag="ACHK3A_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO8738668.1"
                     /translation="MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKR
                     NTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKR
                     LDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQT
                     VCFEGVITINDAPALIDLVQQGIGPAKSMGCGLLSLAPL"
     gene            complement(65206..65880)
                     /gene="cas5e"
                     /locus_tag="ACHK3A_000531"
     CDS             complement(65206..65880)
                     /gene="cas5e"
                     /locus_tag="ACHK3A_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO8738669.1"
                     /translation="MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACL
                     GIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHE
                     TIQTWREYLCDASFTVAIWLTPQATMVMSELEKAVLKPRYTPYLGRRSCPLTQPLFLG
                     TCQASDPQKALLNYEPVGGDIYSEESVDGHHLKFTVRDEPMITLPRQFASREWYVIKG
                     GMDVSQ"
     gene            complement(65883..66974)
                     /gene="cas7e"
                     /locus_tag="ACHK3A_000532"
                     /pseudo
     CDS             complement(65883..66974)
                     /gene="cas7e"
                     /locus_tag="ACHK3A_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            complement(66987..67469)
                     /gene="casB"
                     /locus_tag="ACHK3A_000533"
                     /gene_synonym="cse2"
     CDS             complement(66987..67469)
                     /gene="casB"
                     /locus_tag="ACHK3A_000533"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO8738670.1"
                     /translation="MADEIDAMALYRAWQQLDNGACAQIRRVSEPDELRDIPAFYRLV
                     QSFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKTEQTTGISLGRALANSGRINERRI
                     FQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA
                     "
     gene            complement(67462..68970)
                     /gene="casA"
                     /locus_tag="ACHK3A_000534"
     CDS             complement(67462..68970)
                     /gene="casA"
                     /locus_tag="ACHK3A_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO8738671.1"
                     /translation="MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDME
                     LATLALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQ
                     TKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
                     GFKSGLRGGTPITTFVCGIDLRSTVLLNVLTIPRLQKQFPNELHTENQPTWVKPVKPN
                     ESVPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGL
                     WPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQF
                     RNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTAL
                     RKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANINFSQADEVIADL
                     RDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG"
BASE COUNT        16470 a        18750 c        17633 g        16231 t
ORIGIN      
        1 tcaagtaaga gatggtgcat ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg
       61 agtactctat ccagctgagc tacggatgca tcgggaaact tactttactg cagatttttt
      121 gataccgcta ctaaagccgt atcaagtaag agatggtgca tccgggagga ttactcgcct
      181 tgcggctcgc ccttcgggcc gttgctaaag caacgttatc ctccctggtg cctgcggtta
      241 ttaccgccac ctcttccata ccttcatcgc tgaaggtacg tagaatatgg tgcatccggg
      301 aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc tgagctacgg
      361 atgcaaatgg cggtgaggcg gggattcgaa ccccggatgc agcttttgac cgcatactcc
      421 cttagcaggg gagcgccttc agcctctcgg ccacctcacc acacgcctct tacgagtgct
      481 tcgaagaact tgtttatcgc tcatcgtcgc tgcgtggcgc acatattact ttctgagact
      541 tataagtcaa acaatttttc ccacactttt atcgtttgca cacttcacgc tcaattagtc
      601 tgtaaaaacg tcaaaaagag tgttttatca acagaaaaac ggaggtctgt cagatagcag
      661 taatgcaaaa aaatggagac ttaagttgaa tgaacgggag taaagcgaaa agacaatgga
      721 gtgaagaaga aattttgagg gtgcgtctca ccgataaaga tgagacgcgg aaagattagt
      781 aactggactg ctgggatttt tcagcctgga tacgctggta gatctcttca cggtgaacag
      841 aaacttcctt cggggcattt acgccaatac gtacctggtt gccctttacc cctaaaactg
      901 tcacggtgac ctcatcccca atcatgaggg tctcaccaac tcgacgagtc agaatcagca
      961 ttctttgctc cttgaaagat taaaagagtc gggtctctct gtatcccggc attatccatc
     1021 atataacgcc aaaaagtaag caatgacaaa cacattacat ctaagcagtc atggcattac
     1081 attctgttaa acctaagttt agccgatata cacaacttca acctgacttt atcgttgtcg
     1141 atagcgttaa tgcgaatgcc gtgaagcgag tccacggcat tgcctgacgc ttatattatt
     1201 gcaatttcgc gctgacccag cctttcacac tggctaacgc tgcaggtaag gccgcagcat
     1261 ccgtaccacc ggcttgcgcc atgtcaggac gtccaccacc cttgccgccc acctgctgag
     1321 cgaccatacc aatcagttcc cctgctttca cacgatctgt gacgtcctta gatacgcctg
     1381 caatcagaga aaccttacct tcggctaccg ttgccagcac gataattgtc gaccccagct
     1441 gattttttaa atcgtcaacc atggtacgca acattttcgg ctcaacaccg ctaagctcgc
     1501 taaccaacag cttaacacca ttaacatcaa ttgccttact ggaaagattt gcgctctcct
     1561 gtgcggcagc ttgttcttta agctgttgta actctttttc cagctgacgc gtacgttcca
     1621 gtactgagcg cactttatca gccagattat tgctatcgcc tttcagcaga tgcgcgactt
     1681 cgtttaagcg atcgctgtct gcatgaacgg tggcgatagc gccttctccg gttaccgctt
     1741 cgatacgacg aacgcctgca gcagtacccg attcagagat gatgcggaac agaccaatat
     1801 caccagtgcg actggcgtga gtaccgccac acagctcggt ggagaaatcg cccatgctca
     1861 gcacacgtac acgttcatca tacttctcgc cgaacagcgc catcgcacct ttcgctttcg
     1921 ccgcttcgag atccatgatg ttggtttcaa tcggcaagtt acggcgaatc tgtgcgttca
     1981 ccaggtcttc gaccgcacga atctcttctg gtttcatcgc ttcgttgtgt gagaagtcga
     2041 agcgcagcac tttgtcgtta accagtgaac ctttctgcga tacatgagta cccagaacct
     2101 ggcgcagcgc agcgtgcatc aggtgcgttg cggagtgatt cagacgaata cgggcgcgac
     2161 gagcttcatc aacatcagcc tgcaccgcgt cgcccacttt cagagaaccc gcagcaagtt
     2221 taccgatgtg accaatcgcc tggccgtatt tctgcgtatc ttccaccgca aaggagaagt
     2281 tagcgccttt cagttcgcct ttatcaccaa cctgaccgcc ggattccgca tagaatggcg
     2341 tttgatccag cacgaccaca gcttcctggc ctgcattgat ggcatcaacc gctttaccat
     2401 caacaaacag cgcggtcact ttgccgttca gttccagatg gtcatagcct ttaaattcag
     2461 atgcactgtt aacacggatc attgcgttgt aatcggcacc aaagccgctg gcttcgcgcg
     2521 cacgacgacg ctgctcttcc attgctgctt caaatccagc ttcgtcaact ttgatgttgc
     2581 gctcacgaca aacatcagcc gtcaggtcaa ccgggaagcc ataggtgtcg tacagacgga
     2641 aagccgtttc accatccagc gtatcaccag aaagttttgc cagctcttcg tccagcagcg
     2701 ccagaccgcg ctccagagta cgggcaaact gctcttcttc agtcttcagc acctgctcaa
     2761 cctgcgcctg ctggcgtttc aggtcttcac ccgcagagcc cattacgtcg atcagcggac
     2821 caaccagttt gtagaagaag gtttctttcg cgcccagcat attgccgtga cgcactgcgc
     2881 gacgaatgat acgacgcagt acataaccac ggttttcgtt ggacggcatt acgccatccg
     2941 cgatcaggaa cgcacaagaa cgaatgtggt cagcgattac gcgcagcgat ttgttgctca
     3001 aatcggtcgc gccagtgact ttcgctaccg cctggatcag cgtacggaac aggtcgatgt
     3061 catagttaga gttaacgtgt tgcagcaccg cagcaatacg ctccagaccc ataccggtat
     3121 ctacagacgg cttcggcagc ggttccatcg tgccatctgc ctggcggttg aactgcatga
     3181 agacgatgtt ccagatctca atgtagcggt cgccgtcttc ttccggactt cccggagggc
     3241 caccccaaat gtggtcgccg tgatcgtaga agatttcggt gcacgggccg cacggaccag
     3301 tgtcacccat ctgccagaag ttgtcagatg cgtatggcgc acctttgtta tcgccgatgc
     3361 gaataatacg ttcgcgcggg atcccgactt ctttttccca gatttcgtag gcttcgtcgt
     3421 cgctttcata gacggtaacc cacagacgct ctttcggcag ggcaaaccat ttttcgctgg
     3481 tcagcagttc ccatgcaaac tgaatggcat cgtgtttgaa atagtcgccg aagctgaagt
     3541 tgcccagcat ttcgaagaag gtatggtgac gcgcggtgta accgacgttt tccaggtcgt
     3601 tgtgtttacc acccgcacgc acgcagcgtt gggaagtggt agcgcgggaa taattacgct
     3661 tgtcgagccc aaggaacaca tccttgaact ggttcatccc ggcgttggta aacaacaaag
     3721 ttgggtcgtt atggggtacc agggagctgc tggcaactac ctgatgtccc ttactatgga
     3781 aaaagtcgag aaacgcctga cggatctcag cggtgctctt gctcataatt atcctgaaat
     3841 caagctaacg aaatatcgcc accagctaca gcgtgtttta accgccgggc tggtaactga
     3901 aaagtgggaa taagataagt tttcttgact gggaagtaaa atcccgtatg cgttcagtcg
     3961 gcaaaatttc gccaaatatc ctggatatct tccatcagat agccacgata gagcagaaaa
     4021 cgctggatct taactttttc tgaaaagaca gttggcaaag gttcgccata ttttcgcgtc
     4081 gcctgatcgc gcgccagtgc gcaccagtcg atgtcacatt cacgcatcgc tttttctgtc
     4141 gcttcgcggg aaataccttt ctgattcagt tcctgacgaa tacgcgcagg tccataacct
     4201 ttgcggctac ggctggcgat aaagcgcgca acaaatcggc tgtcatcgag atagccatgt
     4261 tcatggcacc aggcgataac gcgctcgtaa tcttctgccg tggcatcaat ctcttccggg
     4321 ccatttttgc ccatgatcgg tgccgcgagt ttacgtcgca attcttgctc actgtgatcg
     4381 cgcaccgcca gaatgcgtac cgcacgatcc aacaggcgag catatgccgg gcgacgggac
     4441 gttgattctg tcatggcata tccttacaac ttaaaaaagc aaaagggccg cagatgcgac
     4501 ccttgtgtat caaacaagac gattaaaaat cttcgttagt ttctgctacg ccttcgctgt
     4561 catctacaga gaaatccggc gttgagttcg ggttgctcag cagcaactca cgtactttct
     4621 tctcgatctc tttcgcggtt tccgggttat ctttcagcca ggcagttgca ttcgctttac
     4681 cttgaccgat cttctcacct ttgtagctgt accacgcgcc tgctttctcg atcagcttct
     4741 ctttcacgcc caggtcaacc agttcgccgt agaagttgat accttcgccg tagaggatct
     4801 ggaattcagc ctgtttaaac ggcgcagcga ttttgttctt caccactttc acacgggttt
     4861 cgctccccac cacgttttcg ccctctttca ccgcgccgat acgacggatg tcgagacgaa
     4921 cagaggcgta gaatttcagc gcgttaccac cggtagtggt ttccgggtta ccgaacatta
     4981 caccaatttt catacggatc tggttgatga agatcagcag cgtgttggac tgcttcaggt
     5041 tacccgccag cttacgcatc gcctggctca tcatacgtgc cgcaaggccc atgtgagagt
     5101 cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc cgccacggag tcaacgacga
     5161 taacgtctac tgcgccagaa cgcgccaggg cgtcacagat ttccagtgcc tgctcgccgg
     5221 tatccggctg ggagcacagc aggttgtcga tatcaacgcc cagtttacgt gcgtagattg
     5281 ggtccagcgc gtgttcagca tcgataaacg cacaggtttt accttcgcgc tgcgctgcgg
     5341 cgatcacctg caacgtcagc gtggttttac cggaagattc tggtccgtag atttcgacga
     5401 tacggcccat cggcagacca cctgccccaa gcgcgatatc cagtgaaagc gaaccggtag
     5461 agatggtttc cacatccatg gaacggtctt cacccaggcg catgatggag cctttaccaa
     5521 attgtttctc aatctggccc agtgctgccg ccaacgcttt ctgtttgttt tcgtcgatag
     5581 ccatttttac tcctgtcatg ccgggtaata ccggatagtc aatatgttct gttgaagcaa
     5641 ttatactgta tgctcataca gtatcaagtg ttttgtagaa attgttgcca caaggtctgc
     5701 aatgcatacg cagtagcctg acgacgcacc gcatcacggt caccgctgaa acattcccgc
     5761 cgggtaatgc cttcaccacg ggcagtggca aaagcaaacc agacggtgcc gacaggtttc
     5821 tcttcactgc cgccatccgg cccggcgata ccactaatag acacggcgta atcagcacga
     5881 gccgctttca gtgcgcctat caccatttcc accacgacag gttcgctcac cgcgccatgc
     5941 tgtgccagcg tctcttcgcg tacaccgatc atttgcgctt ttgcttcgtt actgtaggtg
     6001 acaaatccgc gttcaaacca ggcggaactg ccggcaatat cggtaatcac tttcgctacc
     6061 caaccgccgg tacaagactc agcagttgtt acggttgcgc cacgggcttt cagcgactgc
     6121 ccaacctgtt cgcttaactg catcagttca ctgtcagtca tcacaacctc tgtcagtcaa
     6181 aaatttatgc cggacaagat aacactttcg tcaggaagat ggtgttggcg ttgcaatttt
     6241 acgaggagga ttcagaaaaa agctgattag ccagagggaa gctcacgccc ccctcttaca
     6301 aatagttact gtacgcgcgc cagcgccacg gcttgtccta actgccagac cgccattgcg
     6361 taatgggtgc tgtggttata acgggtaatg gtgtagaagt tcggcagacc gtaccagtac
     6421 tggtagccgg tgccaacatc cagacgcagc aggctggctt gttgatggtt gcccagcggc
     6481 tgctgtggcg ttaaacctgc cgcggcaagc tgcgaaatgc tgtacttggt tttgaagcca
     6541 tttggcaagc ctggagcctg accgtttgcc atcaccgcaa cctgatcacc tttcacccag
     6601 ccgtgcgctt tgaaatagtt cgccacacta ccaatggcat caaccggatc ccacaggttg
     6661 atatgcccgt cgccgctgaa atctaccgca tattgtttgt aagacgacgg cataaactgt
     6721 ccgtagccca tcgccccggc aaaggaacct ttcagattga gcggatcgtc ctgctcgtcg
     6781 cgcgccatca gcaggaaggt ttccagctcg ccagaaaaat actccgcgcg gcgtgggtag
     6841 ttaaatgaca gcgttgccag cgcatcgagg atgcgagttt tccccatcac gcgcccccag
     6901 cgggtttcaa cgccgataat cccgacgata atttccggcg gtacaccata cacctgccac
     6961 gcgcgattca acgcatcttc atactgattc cagaaaacca caccgttctg cacgttgtcc
     7021 ggcgtaataa attttttgcg ataacgcagc catgcgccgt tcggacctga tggcggtttc
     7081 accgatgtgg ttggtgcctg gttatccatc agccgcagta ccgaatccag acgtttcgcc
     7141 tgggagagaa tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac cattttgtcg
     7201 atgaactgct gggcattcgg attattggcg aagtcaccgc ccatctgcat cacattgtgc
     7261 tgcggctcaa gcaggaagcc gccagacggc gttccggtgg tcgtttcagt ctcagtaggt
     7321 tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt tacataacga
     7381 cgcttgaaca tgaggggtcc atttaacaga ttcaaccagg ggcaagtatg gtaaagcatc
     7441 acgccccgca caaggaagcg gtagtcactg cccgatacgg actttacata actcaactca
     7501 ttctcctcgc tatcctttta ttcaaacttt caaattaaaa tatttatctt tcattttgcg
     7561 atcaaaataa cacttttaaa tctttcaatc tgattagatt aggttgccgt ttggtaataa
     7621 aacaataaat cctgaaggag agaacaatga tagaaaccat tactcatggt gcagagtggt
     7681 ttatcgggct gttccaaaag ggcggagagg tgtttaccgg gatggtgacc ggcattcttc
     7741 cgctgttgat tagtctgctg gttatcatga acgcactgat taattttatc ggtcagcatc
     7801 gtattgaacg ttttgctcaa cgttgcgccg gtaaccctgt ttcccgttac ctgctgttac
     7861 cgtgcattgg cacgtttgtc ttttgcaatc cgatgacctt aagcctgggt cgctttatgc
     7921 cggaaaagta caaacccagc tactacgcgg ccgcctctta tagctgccac tcaatgaatg
     7981 gcctcttccc ccatatcaac ccaggcgaac tgtttgttta tcttggcatt gccagcggtc
     8041 tgacaacact gaacctgcca cttggcccac tggcggtgag ttatctgctg gttggtctgg
     8101 tcaccaactt cttccgcggc tgggtgaccg atctgaccac cgccattttt gagaaaaaga
     8161 tgggcattca acttgaacaa aaagttcatc tggcaggagc aacatcatga cgcgtattcg
     8221 gatcgaaaaa ggaacgggtg gctggggcgg cccgcttgag ctggaagcca cgccgggcaa
     8281 aaaaatcgtc tatatcaccg ccggtacccg gcctgcgatt gttgacaaac tggcacagct
     8341 tactggctgg caggctattg acggatttaa agaaggtgaa cccgcggagg cggaaattgg
     8401 tgtcgcggta atcgactgtg gcggcacatt acgctgcggc atttatccga aacggcgtat
     8461 tcccaccatt aatatccact cgacgggcaa gtccggtcca ctagcgcaat acattgtaga
     8521 agatatttat gtctctggcg taaaagaaga aaacatcact gtagtgggtg atgcgacacc
     8581 acaaccctct tccgtgggcc gtgactatga caccagcaag aaaatcaccg aacaaagcga
     8641 tggtttactg gcgaaggtgg gaatgggtat gggttctgcc gttgccgtgt tgtttcaatc
     8701 tggtcgtgac accatcgaca ctgtattaaa aaccattctg ccgtttatgg cattcgtttc
     8761 ggcgcttatc ggcatcatta tggcttccgg tcttggcgac tggattgccc acggtcttgc
     8821 tccgctggcg agccatccac tgggtctggt catgctggcg ctcatctgct ccttcccgct
     8881 gctttcacct ttcctcggcc caggcgcagt tatcgcacag gttatcggcg tattgattgg
     8941 cgtgcagatt ggtctcggca atattccgcc gcatctggct ttacctgcac tgtttgccat
     9001 caacgcgcag gcggcctgcg acttcatccc ggtcggtttg tcgctggcgg aagcccgtca
     9061 ggacacggtt cgcgtcggtg tcccttctgt tctggtgagc cgctttttaa ccggtgcgcc
     9121 aactgtactg atcgcctggt ttgtctccgg ttttatctat caatagaggc tgaaacatga
     9181 ccgttattta tcagaccacc atcacccgta ttggcgcgag tgccactgac gccctcagcg
     9241 accagatgct catcaccttt cgtgaaggcg cgccagcgga tctcgaagag tattgcttca
     9301 ttcattgcca cggcgagttg aaaggtgcac tccatcccgg tttgcaattt tcactcgggc
     9361 agcatcgcta tccggtgacc gccgttggca gcgtggcgga agacaacctt cgcgaactgg
     9421 gtcatgtcac cctgcgcttc gatggtttaa acgaagcgga atttccgggc actgtccatg
     9481 tggcaggccc tgtacccgac gatatcgcgc cgggatcggt tttgaagttt gaatctgtta
     9541 aggagtaaaa aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt
     9601 tcctgtgcca cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg
     9661 acaaagccgc aaatgtggca caagaaatta acgccgaata tggtgaaggt atggcgtacg
     9721 gttttggtgc tgacgccact agcgagcaaa gcgttctggc gctctctcgt ggggtagatg
     9781 aaatctttgg tcgcgtggat ttgctggtct acagcgccgg aatagcaaaa gcagccttta
     9841 tcagcgactt ccagctcggc gattttgacc gttcgctaca ggtgaatctg gtgggttatt
     9901 tcctgtgtgc ccgtgaattt tcgcgtttga tgatccgcga cgggattcag gggcgcatta
     9961 ttcagatcaa ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggc tacagcgcag
    10021 cgaaatttgg tggcgtcggg ctgactcaat cactggcgct ggatctggcg gagtacggca
    10081 ttacggtgca ttcactgatg ctcggtaatc tgctgaaatc gccgatgttt cagtcgctat
    10141 tgccacaata tgcgaccaag ctgggtatca aaccggatca agtcgagcag tattacatcg
    10201 acaaagtacc gctcaaacgc ggctgcgatt atcaggatgt gctgaatatg ctgctgttct
    10261 acgccagtcc taaggcgtcg tactgcactg gtcagtcgat caatgtcacc ggcggtcagg
    10321 tgatgttctg attaacagcg gagatccgtt aaggatctcc gcgagactaa taaaatgcct
    10381 gatgcgctac gctcatcagg catacaggac ttccgccact acattaagga aaagttatgg
    10441 tatccgcact catcaccgtc gccgttatcg cctggtgtgc gcaactggcc ttaggcggtt
    10501 ggcaaatttc tcgttttaac cgtgccttcg acacactatg ccagcaaggg cgggttggcg
    10561 tgggccgttc cagcgggcgc tttaaaccgc gggtcgtggt cgccatcgcc ctggacgatc
    10621 agcaacgcat cgtcgacacc ttgtttatga aaggactgac cgtcttcgcc cgaccacaaa
    10681 aaattcccgc aattaccggt atgtatgcgg gtgatttaca gcccgatgtg atctttcccc
    10741 atgatccact atcacagaat gctctatcat tggcgcttaa actgaaacgt ggataatttc
    10801 gttgtgaatg ttacttgctt gcgaagttat cattttgaaa cctaaatcag gtaatcacgc
    10861 ccatgaaacc tcgtcagcgt caggccgcca ttctggagta tctgcaaaag cagggtaaat
    10921 gctcggttga agaattggcg caatactttg acaccacagg cacaaccatt cgcaaagatc
    10981 tggtcattct ggaacatgcc ggaaccgtca ttcgtactta tggcggagtg gtgttgaata
    11041 aagaggaatc cgatccgcca atcgatcata aaacactcat caacacccac aagaaagagc
    11101 tgattgcaga agctgccgtt agttttatcc atgatggcga ttcgatcatt cttgatgctg
    11161 gcagtaccgt tttgcagatg gtccccctgc tctcgcgctt taataacatc acagtgatga
    11221 ccaacagcct gcatatcgtc aatgcgctat ccgaactgga taacgaacaa actatcctga
    11281 tgccaggcgg aacatttcgt aaaaaatcgg cctcatttca cgggcaactg gcagagaatg
    11341 ccttcgagca tttcaccttc gataaattgt ttatgggcac cgacggcatc gatctcaatg
    11401 cgggcgtaac cacgttcaac gaggtttata ccgtcagtaa ggcaatgtgc aatgccgcgc
    11461 gcgaagtgat tttgatggcg gactcatcaa agtttggccg taaaagcccc aacgtagttt
    11521 gcagtcttga aagcgtcgat aagctaatta ccgacgcagg tatcgatcca gcgtttcgtc
    11581 aggcgctgga aaagaaaggg atcgacgtga tcataaccgg agagagcaat gagtgaagca
    11641 ctactgaacg cgggacgtca gacgttaatg ctggagttgc aggaagcaag ccgtttaccg
    11701 gaacgtctgg gcgatgattt tgttcgcgcc gccaatatca tcctgcactg cgaaggcaaa
    11761 gtggtggttt cgggaattgg caaatcgggc cacattggta agaaaatcgc cgcaacgctt
    11821 gccagcaccg gcactcctgc cttttttgtc catccggcag aggcattgca cggcgacctg
    11881 gggatgatcg aaagccgcga tgtgatgctg tttatctctt actccggtgg cgcgaaggaa
    11941 ctggatctga ttattccgcg tctggaagat aaatctatcg cgctgctggc gatgaccggc
    12001 aaaccgacgt caccgctggg cctggcggcg aaagcggtgc tggatatctc cgtagaacgc
    12061 gaagcctgcc cgatgcacct tgcgccgacc tccagcaccg tcaataccct gatgatgggt
    12121 gacgcgctgg cgatggcggt catgcaggcg cgcggattta atgaagaaga ttttgcccgc
    12181 tcccaccctg ctggagcact gggcgctcgc ttgctgaata aagtgcatca tctgatgcgc
    12241 cgtgacgatg ccatcccgca ggtggcgtta accgccagcg tgatggacgc gatgctggaa
    12301 cttagccgca ccggtttagg tctggtggca gtgtgcgatg atcaacgact ggtgaaaggc
    12361 gtctttaccg acggtgattt acgtcgctgg ctggttggcg gcggcgcact caccacgcca
    12421 gtcaatgaag cgatgacggt cggcggcacc acgttgcaat cgcaaagccg tgccatcgac
    12481 gccaaagaaa tcctgatgaa acgcaaaatc accgccgcac cggtagtgga tgaaaacggc
    12541 aaactcaccg gcgcaattaa cctgcaggat ttctatcagg ccgggattat ttaatccttc
    12601 agccccagac gtttcgccag ccgatgcagg ttggcgacgt cggtttccag catccgcgca
    12661 caggcggccc agttgtgatg attttgcgcc agtgcctgac gaatggtttc acgctggaac
    12721 gcttctgtcg cttcacgcag gttttgctta acgataggca ccgccgccgc ttctggcggc
    12781 ggcaacgtca cctcaggaaa agcaaaatgt tgcgcctcaa gaatcacttc atcgccgctg
    12841 cgagtggctc tcgacagtac taccgcccga tgaatagcat gttccagttc gcgcacgttt
    12901 cccggaaaac tgtagtgttg cagtaaattt cgcgccccgg cacttaatac cacgcgggag
    12961 agccccagcc gcaaacgaca ctgctcgcag aaataccccg ccagcagaat gacatcatcg
    13021 ccccgctcac gcagcggcgg caccgaaagt ggaaacacgc tcaggcgatg aaacaaatcg
    13081 gcgcggaatc gccctgccag cacctcttcg cgtaaatcgc ggttagtcgc cgccagcacg
    13141 cgcacatcga cccgcaaact gcggtcatcg ccaacgcgct gaatatcgcc atactgcaac
    13201 accctcagca gcttggcctg caatgccaac gacaactcgc cgatctcatc gagaaacagc
    13261 gtgccgttat ccgccatttc aaacttcccg ctgcggttac tgatagcacc agtaaacgcc
    13321 cctttcacat gcccgaacaa ctcactttcc gccacacttt ccggcagtgc ggcacagttg
    13381 agatagacca gcggattcac cgcccgtggc gaggcttcat gaatcgcttt cgccaccagc
    13441 tccttaccgg ttcccgtctc accgctaatt aaaacgttga gatcggatgc cgccacaatc
    13501 tcaatctctt ttttcaattg cgtcatgcct ggggacaaac caatcatctg cgtctgtttc
    13561 accgcttcaa acggcgcggc atcgcctggc agcatattct ggctttccag ttgttcaatc
    13621 agtaacgcat tacttaacgc tcccgccgcc agtgcggcaa tcagccgtag ctcttcgtcg
    13681 ctgaaaacat cgaactgatc gggctgcatc ccgtcgagcg tcaaggcacc gatcaggttt
    13741 tgcccggcaa acaacggcaa accaacgcag gcgtgaacct tcagactctc ctgccctgga
    13801 atcaaaccgt cataaggatc gggcaattcg ctgtctgcgg gaaagcgcac cacatcaccc
    13861 gcgcgggcaa tcgcttccag ccgtggatga ccttccagcg caaagcgtct accgagtaca
    13921 tccttcgcca gcccgtcgat ggcaagcgga ataaactgcc gcgagtcgta acgtagcaac
    13981 gcagacgcat cgcactccag cacctgacgc agcgtggtga tcaggcgctg aaaacgatcc
    14041 tgatgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc aacggaaaaa
    14101 ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc attttattga
    14161 tagtcatttt gactactcat taatgagcac aattttattt atagagtaaa aacaattaga
    14221 taaaaaactg gcacgcaatc tgcaatcagc aagacatctt tttagaacac gctgaataaa
    14281 ttgaggttgc tatgtctatt gtggtgaaaa ataacattca ttgggttggt caacgtgact
    14341 gggaagtgcg tgattttcac ggtacagaat ataaaacgct gcgcggcagc agctacaaca
    14401 gctacctcat ccgcgaagaa aaaaacgtgc tgatcgacac cgtcgaccat aaattcagcc
    14461 gcgaatttgt gcagaacctg cgtaatgaaa tcgatctgac ggatatcgat tacatcgtaa
    14521 ttaaccatgc tgaagaggac cacgccgggg cgctgaccga actgatggca caaattcccg
    14581 atacgccgat ctactgtact gccaacgcta tcgactcgat aaatggtcat caccatcatc
    14641 cggagtggaa ttttaatgtg gtgaaaacag gcgacacgct ggatatcggc aacggcaaac
    14701 agctcatttt tgtcgaaacg ccaatgttgc actggccgga cagcatgatg acttacctga
    14761 caggcgacgc ggtactgttc agtaacgatg ctttcggtca gcactactgc gacgagcatc
    14821 tgttcaacga tgaagtggat cagacggagc ttttcgagca gtgccagcgt tattacgcca
    14881 atatcctgac gccgttcagc cgcctggtaa cgccgaaaat taccgagatc ctgggcttta
    14941 acttaccagt cgatatgata gcgacctctc acggcgtggt atggcgcgat aacccgacgc
    15001 aaattgtcga gctgtacctg aaatgggcgg cggattatca ggaagacaga atcaccattt
    15061 tctacgacac catgtcgaat aatacccgca tgatggccga tgctatagcc cagggtattg
    15121 cggaaaccga cccacgcgta gcggtgaaaa ttttcaacgt cgcccgaagc gataaaaacg
    15181 aaatcctgac caatgtcttc cgctcaaaag gcgtgctggt cggcacttcg acgatgaata
    15241 acgtgatgat gccaaaaatc gccgggctgg tggaggagat gactggttta cgctttcgta
    15301 acaaacgcgc cagtgctttc ggctctcacg gctggagcgg cggtgcggtg gatcgtcttt
    15361 ccacacgcct gcaggatgcg ggtttcgaaa tgtcgcttag cctaaaagcc aaatggcgac
    15421 cggaccagga cgctctggag ctatgtcgtg aacacggtcg cgaaatcgcc cgtcagtggg
    15481 cgctcgcgcc gctgccgcag agcacggtga atacggtggt taaagaagaa acctctgcca
    15541 ccacgacggc tgacctcggc ccacggatgc agtgcagcgt ctgccagtgg atttacgatc
    15601 cggcaaaagg cgagccaatg caggacgttg cgccaggaac gccgtggagt gaagttccgg
    15661 ataacttcct gtgcccggaa tgctcccttg gcaaagacgt ctttgacgaa ctggcatcgg
    15721 aggcaaaatg agcaacggca ttgtgatcat tggttcgggc ttcgccgccc gccaactggt
    15781 gaaaaatatt cgcaaacagg acgcctgtat tccattaacc ctgattgccg ccgacagcat
    15841 ggatgagtac aacaaaccgg acctcagcca tgttatcagc cagaggcaac gtgccgatga
    15901 ccttacccgc cagacggcgg gtgaatttgc cgagcagttt aatctgcacc tgtttccgca
    15961 cacctgggta acggatatcg atgccgaagc ccatgtggtg aaaagtcaga ataatcagtg
    16021 gcaatacgac aagttagtgc tggcaaccgg tgccagcgcc tttgtcccgc cagtgcccgg
    16081 gcgtgagtta atgctgacgt taaatagtca gcaagagtat cgcgcctgtg aaacgcaact
    16141 gcggaatgcc cgacgcgtgt tgattgttgg cggtggcttg attggtagcg aactggcgat
    16201 ggatttttgt cgtgcaggca aagcggtcac gctaatcgac aacgctgcca gtattctggc
    16261 agcgttaatg ccaccggaag taagcagccg cttgcagcat cggttgacgg agatgggcgt
    16321 tcatctgctg ttaaaatctc agttgcaggg gctggaaaaa acggattctg gcattctggc
    16381 aacgctggac cgccagcgca gcatcgaagt ggatgcggta attgccgcca ccggactgcg
    16441 cccggaaacc gccctggcac gacgcgccgg gctgacgatt aatcgcggcg tttgcgtcga
    16501 tagttatctg caaaccagta atgccgatat ttatgcgctg ggcgattgcg cggaaattaa
    16561 cggtcaggta ttgccgttcc tccagccgat tcaacttagc gcgatggtgc tggcaaaaaa
    16621 tcttctcggc aataacacgc cgctgaaact cccggcgatg ctggtgaaaa tcaaaacgcc
    16681 ggaattaccg ctgcatctgg caggcgaaac ccagcgtcag gatttacgct ggcaaattaa
    16741 taccgaacgc cagggaatgg tggcgcgcgg tgttgatgat gccgaccagc ttcgcgcctt
    16801 tgtggtcagt gaggatcgga tgaaagaggc gtttggattg ttgaaaacgt tgccggtgta
    16861 ggcgagctac agtgccccaa atgtcggatg cggcgctgac gcgtcttatc cgacctacag
    16921 ggaacgcatg tgttgatcac ataatacgtt tatgtcgcat cctgcactgt cccaaaatgt
    16981 cggatgcgac gctggcgcgt cttatccgac ctacggggac gcacgtgtag gccggataag
    17041 gcgtttacgc cgcatccggc aatggtgtcc aaatgcaaca cgctttatcc gttctggact
    17101 tcacccgcta accagcgcgc cgcagcaata accccctgcc ccagagacaa tccaccatcg
    17161 cccgccggta aactctgcgg gaagagcaac gtaaaatcag caagataatg cgccagacgt
    17221 gcacgcagca aacggttatg aataactccg ccgctaaata ccagcgtagt gataccccgc
    17281 atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc atgaaacgcc
    17341 cacgcgcgtt gattaaccgg agcctgccag ctcagccact gctgccagaa agtggcgaga
    17401 tccagttgat tgtccaccag cggcatcgtc accggatgcg tcactccgtc gcacgaagct
    17461 gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc tggcgcacag
    17521 cccagtcccg ccgccacagc atcgaacaaa cgcccacacg atgacgccag cggcgcgtta
    17581 attccacgct caatggcccg cgccagcacg ctccagtttt gctgttgcac acttgccgtt
    17641 tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag gttacgccac
    17701 ggctgcttcg ccgccaaatc gcccccagca agcgccactg caggcaagcc gcccaggtgc
    17761 tggcattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc gttctccccc
    17821 ataccgatac cgtcgagcgt caacgcaatg acatcaccgc catccagtgg ccagtgatgt
    17881 tctgccagac acgccgccgc atgagcatga tgatgcagca ccgtttgcgt cggcagattc
    17941 atctcacgcg cccactggct ggagacatag cccggatgcg cgtcatgcac aacgtattgc
    18001 ggggtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg catctggatg
    18061 ccatcgtcac ttaaatcgcc cagatgctgg ctcaacaccg cttgttcacc gcgcaccagg
    18121 cagaaggtat ttttcagatc cgcgccgaga cacagcacag gcggaacatt tttaaagccc
    18181 ggaggcaaag ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat ttcgccgctt
    18241 tcgcgcacca ccgaatcatc catccgctgc acgatgtcgc ggttatgtat caagaatccg
    18301 tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagccgg tggtttaccg
    18361 ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag caaatgctgg
    18421 agcgggttcg caggcaacat caccccaact tcgttaaggc caggggcgat atcatcacaa
    18481 agctcaggaa cgtatttttt atccaccagc acaatcggcg cggcgggtgt ggtaagcaac
    18541 tgacgggcag cgtctggtaa accttctgcc actggcaaca taaccgccag cggtttcgcc
    18601 gggcgatgtt tgcgcgcccg cagtgtcgcc accgcgttac tgttacgcgc atcgcaggcg
    18661 agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa ctgtgcgata
    18721 gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca ttcaagatgg
    18781 gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag cgggtcacgg
    18841 tactctttgt cacaggccgg acacagcgga aacgccgcca tcacggtaaa cgggcggtcg
    18901 taaggcatgg cgcgaataat ggtgaaacgc ggaccacagt gagtacagtt gataaacgga
    18961 taacgccagc gtcgttcgcc tggggtattc atttcggcaa gacaagcagg gcaagtcgcg
    19021 gcatccggga caatttgcgt attcatgacg ccacccgcgc tctggcggat ggtgaactca
    19081 gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg cgccagtggc
    19141 gggcagtgct gatgcaactg cacaagaaac gtttccggat cttccagcag ccggacttct
    19201 acgccatcgc cgtcattaca gacatcgccg tgaagattta attgctgtgc cagttgccag
    19261 acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg gacaccgcaa
    19321 gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc cgcccagccg
    19381 gacacgacag cgtcgcatcc ggcagtcaca ggtcggcgat actgcccgct tcgtattcta
    19441 cgaatatttc cgggaattcc tttgatgcca gaacagttca gtaagatttt tagaacatca
    19501 gcgccgtacg gcgacgtttt tctgcgctca gttgttcaag tttattacga tcgacacaaa
    19561 tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga tggttgcaca
    19621 ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagccccgcg ccgctgttgc
    19681 gaatcaccgg acgtaccacc acttccatcg cgccatacgg gcaagccaca acgcaggttt
    19741 tgcaaccaat acaacgttcc tgcatcacat gaacaaaccc tttatcacga ctgatagcac
    19801 cattcggaca gacgttagcg cacggtgcat cttcacactg acggcaaact gtcgccgtgg
    19861 aaatgttcac acctttaatg acatggattc gcggtaaaaa agtttccggg gtcagcgatg
    19921 cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta cggcaaccaa
    19981 tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc aatgacagtt
    20041 aatgcgccga tacattcaca aatcatgcca gtttttaatt tactgttatt taaagaaatt
    20101 aaattctgta atgcaggaaa aacgatgtca tcgacactag tgacgatgac atgtgatgac
    20161 aatgtttatc gcgaaagagc aatgagggag tcgcggcgga tcagttttcc gctgaaggtt
    20221 ttcggcggtg tgtaagcacc accatcgagc ataaatatca gccgcccaat tatttcctga
    20281 atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacggtgta gggggcaata
    20341 gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc gcgctcgtgt
    20401 aacgctttca tcgcacctat cgccatatcg tcgttactgg caactaacgc gctaaattta
    20461 gccccacgtt cgagcaacat ttctacccct tcggccccgc tggcaggcgt ccatttaccg
    20521 ttagcgataa gtttttcatt gagcgcaata ccatgctgcg ccagcgcgtc tttatacccg
    20581 gcaagacgtt caacgctggt gggggaatcc atcgaaccgg taaggaaagc aatctcctga
    20641 tgtccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg atcgcaccag
    20701 acgctatggc tgctgttttt gcgcaggcgg cgattaagca ccattatcgg ctgactgtgt
    20761 gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat cataatggcg
    20821 tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc gctgtgttta
    20881 ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc atgaaagagt
    20941 aattcactaa aataaatgcc gtggtaaagc gtgttggtca cgaccagccc cagcgtctga
    21001 gtactcttcg ccgacagatt gcgcgccagc aagtttggac ggtaaccgct ctcttctacc
    21061 gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga aagcacgcgg
    21121 gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt cgtcatcatt
    21181 ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt gtccttgcgc
    21241 aatcggcggg aaaaatattc aggtgaccgg tttcacaaat ataaaaaatg aacaattaac
    21301 tctcttgctt attcagtgac aactattcat gattttgtga aaccggtttc ctaattccgt
    21361 ttcagcatcg gcatttttcc gtcacgtcga ctgataacaa ctacatctac cctactgata
    21421 acaggataaa atccgatggc caaaaattat gcggcgctgg cacgctcggt gatagcggca
    21481 ctgggcggcg ttgataacat ctcggcagtc acgcactgta tgacgcgttt gcgctttgtt
    21541 atcaaagatg accagcttat cgacagtccg acgttgaaaa ccatctccgg cgtgctcggc
    21601 gtggtacgta gtgacaacca gtgtcaggtg attatcggca ataccgtttc acaagccttt
    21661 caggaagtcg tcagcctgct gccgggagat atgcagcccg cacagcccgt gggtaaaccc
    21721 aaacttacgc tacgtcgcat aggtgcgggg atcctcgatg cgctgatcgg caccatgtca
    21781 ccgctgatcc cggcgattat cggcggatcg atggtcaaac tgctggcgat gatcctcgag
    21841 atgagcggcg tgctgacaaa aggatcgccg accttaacca ttctgaatgt gattggtgac
    21901 ggtgctttct tcttcctgcc gctgatggtc gcggcttcgg ccgccatcaa atttaaaacc
    21961 aatatgtcgc tggcgattgc catcgcgggt gttctggtac atccgagttt tattgaactg
    22021 atggcgaaag cggcccaggg tgaacatgtc gaatttgccc tgatcccggt caccgcggtg
    22081 aaatacacct acacggtgat cccggcgctg gtgatgacct ggtgcctgtc atatatcgaa
    22141 cgctgggtgg atcgcattac accggcggtg actaaaaact tcctcaagcc aatgctgatt
    22201 gtgttgattg ccgcaccgct ggcaatcctg ctgattggcc cgattggtat ctggatcggt
    22261 agcgccattt cggcgctggt ttacaccatt catggttatc tgggctggct ttcagtcgcc
    22321 attatgggcg cgctgtggcc tctgctggta atgaccggga tgcaccgcgt ctttacgcca
    22381 accatcattc agaccattgc cgaaaccggc aaagaaggga tggtcatgcc gtcagagatc
    22441 ggcgctaacc tgtcgctggg cggttcatca ctggcagtgg cgtggaaaac gaaaaacccg
    22501 gaactgcgtc agacggcgct ggctgcggcg gcatcagcca ttatggcggg gatttccgaa
    22561 ccggcgttat atggcgtggc gatccgcctg aaacgtccgc ttatcgccag tcttatcagc
    22621 ggttttattt gcggcgcggt tgccggtatg gcggggcttg ccagccactc aatggcagcg
    22681 ccggggctat ttaccagcgt gcagttcttc gatccggcga atccaatgag catcgtctgg
    22741 gtgttcgccg tcatggcgct ggcggtcgtg ctgtcgttca tcctcacact gttgctcggc
    22801 tttgaggata ttcctgttga ggaagcgact gccgaggcgc gaaagcatca gagcgcacaa
    22861 ccgaccgtcg ccaaagaagt aagtcttaat tgaggatgaa aatgtcagta tttccagaag
    22921 gttttttatg gggcggcgcg cttgccgcca accagtctga aggtgcgtac cgggaaggtg
    22981 gcaaagggct gaccaccgtc gatatgatcc cacacggcga gcatcgaatg gcggtgaaac
    23041 tggggctgga aaaacgtttt cagttgcgcg atgacgagtt ttatcccagc catgaggcga
    23101 cggattttta tcatcgttat aaagaagata tcgccctgat ggcagagatg ggattcaaag
    23161 tgttccgtac ctcaattgcc tggagccgcc tcttcccgca gggcgatgaa ctgacgccca
    23221 accagcaggg cattgctttt taccgttctg tctttgaaga gtgtaaaaag tacggtatcg
    23281 aaccgctggt cacgttgtgc cacttcgatg tgccgatgca tctggtcacc gaatatggct
    23341 cctggcgtaa ccgcaagctg gtggagtttt tcagccgcta cgcccggacc tgctttgaag
    23401 catttgatgg tctggtgaaa tactggctca ccttcaatga aatcaacatt atgttgcata
    23461 gcccgttctc cggcgcgggt ctggtgtttg aagaaggcga aaatcaggat caggtgaaat
    23521 atcaggctgc gcatcaccaa ctgattgcca gtgcattagc caccaaaatc gcccatgagg
    23581 ttaacccgca aaatcaggtg ggctgtatgc tggcgggcgg taacttctac ccttacagtt
    23641 gcaagccgga agatgtctgg gcggcgctgg agaaagatcg ggaaaacctg ttttttatcg
    23701 atgtgcaggc gcggggcgcg tatccggctt actctgcccg cgtattccgc gaaaaagggg
    23761 taaccatcga caaagcaccg ggcgatgatg agatcctgaa aaacaccgtc gattttgtct
    23821 ctttcagcta ttacgcctca cgctgcgcct cggcggagat gaacgccaac aacagcagtg
    23881 cggcgaacgt ggtgaaatcg ctgcgtaacc catatctaca ggtaagcgac tggggctggg
    23941 ggattgatcc actcggtctg cgtatcacca tgaacatgat gtatgaccgc tatcagaagc
    24001 cgctgtttct ggtggaaaac ggcctcggcg caaaagatga acttgctgcc aatggcgaga
    24061 ttaacgatga ctatcgcatc agctatttac gcgaacatat ccgcgcaatg ggcgaagcga
    24121 ttgcagatgg cattccgctg atgggctaca ccacttgggg ctgtattgat ttggtttccg
    24181 cctcaacggg tgaaatgagc aaacgctacg gttttgtcta cgtagaccgt gacgacgcag
    24241 gcaacggtac gctggcgcgc acgcgtaaga aatcgttctg gtggtataaa aaagtgattg
    24301 ccagtaatgg tgaggattta gagtaagtaa cagtgccgga tgcggcgtga acgccttatc
    24361 cggcctacat gtcgcacgaa ccaggtaggc ccgataagcg cagcgcatcg ggcaatctgg
    24421 cgtttgctat cagttctgat tccctcatca agaacatccc tgtcctgatt ccttactgaa
    24481 aaactactct tcttccaccg ctaactgcgc gaatccgcca ttcccttccc agccttccag
    24541 tcgttgataa acggtttcta ccgcatcttt aatcggctgg gtcatcgggt agtaaaagcc
    24601 gacgatatcc ggctggatgc cgaggaaaat cacttcgcca atgtcttctt ttaactggtc
    24661 gataaggtag ttcaacggca tgttatgggt agtcatcata aacatctcgg cgatatcatc
    24721 cggatcgatg atgcggatct cgccggggtt taaccccata tccgtggcgt cgacaatcag
    24781 cagccgtgtc gggcgcagtt cgcggatggc gacgatgtcg ttttccggag cgctaccgcc
    24841 gtcaatcacc acccagttac ctttcggcgc ggcggcgcac ttttccgcca gcagcgggcc
    24901 tgcgccatca tcgcccatca tgctattgcc aacacaaagt aaaacgtcag tcacgcagtc
    24961 tcctcaccat caaatagatg gcgttttcct gatgaatatc atgcagcata cttaacagca
    25021 ttgtactcca ttctttctgc tgcgaagttt gcgctgcccg ggctttatcg aatgcgttag
    25081 ccagcatcgg cacatggtta atgtcgatga cgatctcacc atatttcggc acgccttcca
    25141 ttttgcggcg ggcttcgctg cccgcctcca gagtggcaat ccatgccaga tagtcatccc
    25201 acgggcaggt aagcgccgct tccaggcaat cgataacccc aaggtggtga ccaatcgcca
    25261 ggctgtaata gaccacctgc tgcgcctcgg cgggcgtagc atcgttctca tcaataaatt
    25321 tacggctcag ttgactgaac accacctttt cactcatcgg atacgcgcct cttcaacaac
    25381 atgattcaga tggctgacaa tctcgttcag acgcggatcg ttttccgctt ccagccagcg
    25441 tgcaacctgt tcttcgccct gccctaactg tgtaaggtaa tcatcggcaa tctgacgacc
    25501 gtaacgataa cccgccaggc ggcgtgcttc gcgatccact ttcacacgca gcggctgcac
    25561 catatcgcca tgcaggatct ccgccggttg ttcatcctgc tcacccggcc cacgggcgtg
    25621 aattttctgc tccagcaggc cgagcgccat tgcaaagccg tatagcgtag cggcaggcgt
    25681 tggcgggcag ccagggatgt agacatctac cgggacgatt ttatccgtac cgccccacac
    25741 gcagtagaga tcgtggaaga tcccgccact gttaccgcag gcaccgtagg agatacagat
    25801 tttcgggtcc ggcgcggact gccacgcacg cagcgcaggg gatcgcattg cacgggtgac
    25861 cgcgccggta aacagtaaaa tatccgcatg acgcggtgaa ggaacgactt taatgccgaa
    25921 gcgttctgca tcaaacagcg gcgaaagcgt ggcgaaaatt tcgatttcgc agccgttgca
    25981 gccaccgcag tccacgcggt aaacgtaggc agaacgtttg atttttttca gtaacgacgc
    26041 cttcatgctg gcgatggatt catccaccgt catcgggacc ggaatgccgt tggcgtcacg
    26101 ggggcctaat aaattgctca tcagatggcc tctttcatat ggcgagtcag ttcaatacgg
    26161 tcggacggca ccaggcattt ctggcgctta cattccgggc aagtctcaaa gctttcgcgg
    26221 tggttttccg cgcggctgtc accattgtgc ttaagcagcg caatggcgta gtcgatctct
    26281 ttctggacgg cgaaaggacg attgcagacg cggcagttgc acagcgcgaa gcgggactgt
    26341 tggaggaagt cttctttctt ccacaccgcc agttcgtact cttgcgacag tttgatcgcc
    26401 gccgtcgggc aaacttcttc gcagcgtcca cagaagatgc agcgcccgag attaaactcc
    26461 caggccagct ctcctgtggc gaggtcggtt tcaaccgtta aggcgtttga cgggcaggca
    26521 ttgacgcagg ccgcgcagcc gatgcactgc tgcgggttct gctctggctt accacggaag
    26581 tttttatcaa ccgcaatcgg ctccagcgga taagacgagg tcgccgtgcc ggttttgatg
    26641 acttttttga taaaggtaaa catggcgatt ccttatttca gcggcgagtt tttacgctca
    26701 atgctgtaac gctcgagttc tttgtacggc accactttgc tcttcttctt acgcacatcg
    26761 accacggtca tgcggtcggt acaggagtag caagggtcca ggctaccgat aatcagcggc
    26821 gcatcggaaa cggtgttgcc gcgcagcatg tagcgcaggg tcggccagtt cgcgtaggtc
    26881 gcggcacggc agcgccagcg gtacagcttc tggttgtcgc cggtcatgct ccagtggata
    26941 tcgtcgccgc gcggcgcttc ggcaaagccc agcgcaaaac ggtgcggaat gtaggtaaaa
    27001 ccttctacca tcaacggacc gcccggcagg ttatccagac cgtagtcgat catgttcagc
    27061 gcggtataga cctcgttgat tcgcactttc agacgggaaa taacgtcgca gccctgctcg
    27121 ctgtggactt ccattggcag caggccataa ccgacaaacg ggtgatcggc gcgagtatcg
    27181 cgggcgtgac cgctggcgcg aaccatcggg ccaacgttac tgaaatcgcg ggcgatttcc
    27241 gggtccagac gaccaatgcc gacggtgcgc tgttccatgt tcggtgtgct cagcagcaca
    27301 tccaccagct cctgcacttc acgacgcatc tgttgtgcca gctggcgggt ctggatcatg
    27361 tcgtctttca gcagatcgcg acgaatcccg ccgatcaggt tcaggccgta ggttttacgt
    27421 gcaccggtaa ggatctctgc cattttcatg gaggtttcac gcacgcggaa gaactgcata
    27481 aagccggagt cgaagccggt aaagtgacag gcgaggccga gattgagcag atgcgagtgc
    27541 aggcgttcca cctccaacag aatggcgcgg atcatctgcg cacgttctgg cacctgaata
    27601 cccatcgcgt tttccaccga cgtggtgtag gcggtgctgt gagcaaagcc gcagatcccg
    27661 cacacacggt cagagaggaa ggtcacttcg ttgtaaccca tacgggtttc cgccagtttt
    27721 tccatgccgc gatggacgta gaacaggcgg tagtcggcgt cgataatgtt ttcaccatcg
    27781 acaaacagac ggaagtgacc cggttcgtca gaagtgacgt gcaggggacc aatcggcacg
    27841 acgttgtttt tcttgtcgcc cagttcgttg atgaactcgt aggtttcagc atcggtagtc
    27901 ggtgccggac gctgacgata atccatgctg tctttacgca gaggataaag ttcatccggc
    27961 cagtcatccg gcagcaccag acggcgttca tccggcagac caaccggaat caaaccgtac
    28021 atatcgcgca cttcacgctc gccccacact gccgccggaa cgcgcggcgt cacggaagga
    28081 tactccggtt tgttggcgtc aacttcgacg cgcaccgtta cccaacactt agtccccttc
    28141 tccatcgaca gcacgtaata aacggcgtaa tgaccattca gtttgcgttc gtcgttacca
    28201 aacagcaccg acagccagcc cccctgcttg tagtagagaa actccaccac ttccggcagg
    28261 tagttcacct tcacggtcac ggtcagctga tctttggtct gccaggcgtg gtccagcacg
    28321 acgcccggaa atgcctcatt cagcgcggcg agataatgtt gacctaattt ttcttcagac
    28381 atgctcaaac tctctttaat cacgccgcca gcaaggagac gaacgctaaa aatgcaaagc
    28441 caaaaccagc ccaggtaatg cgtggagtaa tatcaagacg caaacgggcc atgctgtttt
    28501 cgaacagcgc gataaccagt acgccgacca ccagtttgac gatggcaatc accagcgcca
    28561 gcagcagccc accgacggtg aaggtttcca tttgtcccca cggaataaac accccgacga
    28621 acatctgcaa caccaccagc tgtttcaggc tgatgcccca tttcatgacg ccaaagccgc
    28681 tgccgctata ctcagagagc ggaccttcct gcaactcctg ctcggcttcc gccaggtcga
    28741 acggcagttt gcccatttcg ataaaagtgg cgaacgcaca ggcacaaagc gccagcacca
    28801 gcgggatgct ctggctcagt ggccagtgat aaacggtgtc ggtgatgttg ctgatgttgg
    28861 tggaaccggc aacctgtgcg gcgacccaca gaccaagcag cagcatcggt tcaaccaaca
    28921 cgccaagcat cgcttcacgg ctcgcgccga tagcggtaaa cgggctacca gtatccagac
    28981 cagaaatggc aaagaagaaa cgcgcgatgg cgaagagata cagtaaggtg atcaaatcgc
    29041 ccagttgcgg cagcggagag ccgacggtca ccaccggcag cgcagtggcg atagtcagca
    29101 tgacgcccac catcacatac ggcgtcaggc ggaacaccca gccggaggca tccgggccaa
    29161 cgctctgacg ccccagcagt ttgataatgt cgcgatactc ctgcaacacg cccggcccac
    29221 gacggttatg caagcgggcg cgcgctacgc gggtaatacc ggagagcagc ggcgcaacgg
    29281 caaataacac cagcgcctga attaacggat ataaaacact cattctcagg ctcctcgtga
    29341 aacaataatc accaccagta ccgccagttc aaccagcgcc atccggcgga acagcaacgc
    29401 actcccctcg cactgccagc ccggcaccag cgacaccgga ttcagccatt tgcgtagttt
    29461 cagcaccggc gcaaacgcct gtttcaccgg catggcaaaa ccgtgagcgg taatcaccat
    29521 tgatttttca tgatcgtagc cgcacaccca ggccgcgccg cgggaacgcg acggcaaacg
    29581 atcgcctttg caaatcgcca taatgatgaa tggcagcagc gggcaggcaa tcagcagcaa
    29641 cgtgatcatg ggttgagaaa cggtggtgtt agcaggctcc agcggcagag gtacagcagc
    29701 agagagcatc ggcagtagcc acggcgcagc aacaccgcca attacgcagc aaatcgccag
    29761 tgccactacg cttacgctca tcaggagcgg cgcacaggtg gcgttttcgg cctctttggt
    29821 acgcggcgcg ccgaggaaag taacgccata aactttcgcc atacacatca ccgccagcgc
    29881 accggtaatt gccagcccca cggcgagcag cggcccgaga agacgggcaa caaacgcgcc
    29941 actattgctc agtttgaaga aggattgata gataacccat tccccggcaa acccgttcag
    30001 cggcggcagc gcagccattg ccatcagccc gactaacatg gcgatggaga taaccggcat
    30061 tttcttgcca ataccaccga gtttttcgat atcgcgatga ccggtacgga accagacgct
    30121 ccccgcacca aggaacagta cgctcttgaa caggctatgg ttaagcagat ggtacagacc
    30181 accaaccaga ccaagggcaa tcagcgccgg ttgttcgagc gcgatacccg ttacgccagc
    30241 gcccagcccc agcaggatga tgccgatatt ttccagggtg tggtaagcca gcagacgttg
    30301 gatattatgt tccatcaacg catacagacc gccgacgaat gcggtgatca tgccgagtac
    30361 cagcagtgcg atcccccacc acagcggtgc attaccgccc agtagtgaca gagttaaaat
    30421 gcccagcagg ccaattttca tgactaccgt ggaaaacagc gcggcagctg gcgcagaggc
    30481 gttggcatgt gcctgtggca cccagccgtg cagcggaata atcccggcca gcaggccaaa
    30541 gccaatcaca ccgagcagcc agatatcgga accgagcggc agctgttgca tacgcatatc
    30601 cagcaggcgc agatccagcg tgccgtaacg ctgccacagc agccagcagg caatcgccag
    30661 cagcagagtg ccaagacgcc ccagcgcaaa ccacagtttg ccctctttgc tgttgctggt
    30721 gaggaacacc gcacacaggg ccatgatttc ggccattacc acgaacatgc cgaggttgct
    30781 ggcgatgacg gcgcagacgg cggcagccat caacatattg atctgcaagc cgttgcactt
    30841 cacctgcgcg tggcgatgcc agtcaatgtt gtagaggctg acaaacagac cgcataggcc
    30901 gagcgtaatc agccaaatcg cattaagcgg agagatttgc acatcgtagc ttaccagcga
    30961 cagcgcaccg ctcacgccaa ccgcgccagt cagcactgtg aagcccgcgg ctgccgtata
    31021 caggctacca accgcgccgc caattccggc tatccagccg cttagcgcct tttgaaaaga
    31081 aaagagaaat gccagaacag cggcggcgac aaaccacgcc acgccgctat tgatcaggga
    31141 aattgcgctc atttagcctc tccactttga gcctgctgaa acaaggtgag atcgccaaaa
    31201 tccgtgttaa aagtcagctc acgcttacgt ttgctgacgc gggcgatatc ggtgttatcc
    31261 accagatgca gggctttagt cgggcacatc cgcacgcagg ccggaccttg ttcatcaaag
    31321 ctacaaaggt cacatttgac ggcgatcgcg cgaatacctg gcacccagtc aagcaatgtg
    31381 ctgacacgcg ccggagcagg cggtgccggt ggcgctttcg gggtattggc gtttgccgga
    31441 atatccagcg gacggctgcc ggaaaattca attgcgccaa acgggcaggc gatgccgcac
    31501 agtttgcagc ttacgcacag actttcattc aactgcacgg ccccatcaac gcgggtgatg
    31561 gcgttaaccg ggcaaaccac cgcacagggc gcatcttcac agtggtgaca gagctgcggc
    31621 gcagattctt tttcattgag catcactcgc aggcgcggca ttgattgcag gccgtgctgg
    31681 cgatgcgtct ctgaacaggc ggcctcacag gtgtggcagc cgatacagag cgtggagtca
    31741 gcaattacaa aacgattcac caggcattcc tcaggtgatt gtcatttttg acgaaaacat
    31801 gccgatgaaa tgtcattttc gacactcatc gacacgccca tccccaaaca ggcgtaacgc
    31861 ctgcaaaacg ggcaaagcct cagctcatgc tgccgggctt tgtcccttta ccagttggct
    31921 taaattcacc ggcacattgt tttcaatggc ggtgtataag ctgtcgtaaa cgccagcgat
    31981 tttttcgaga tagtgttcca gaacctgttc gcgatcgcgg atgttgacct tgccatcaat
    32041 catgccgtca atgctcagtt gcgcctgcgc aatccgctgt ttgtcttcac catcttttgt
    32101 ttcgacgtaa tactcgatgg tgtggctgtt aaagccatcc gccagggtga cgtaaatacg
    32161 aaaatgttca aaaaggacga aacagagttc tttgtccggc gcgcagctca tggcgtgatg
    32221 caggcgcgct ttcgataacg tgattccctg aaccagcgaa ttgcagtaga tgtgccactg
    32281 gtcctgtagg cgacgatgcc gctgtgcgat gtaatcggct ttctcgctta tttcccaaat
    32341 agtcattgtc aggttacccg tttaacagag atgccagctt taagcatttt ttgtgccaac
    32401 ttttaattta ttgttattaa agcgattttt aagctaaaga tgaatttcgt cgccgtgtcg
    32461 acgtgtcatt tcgacatcat cgacattatt caccgcaggg ataatcaaca ctggcacaat
    32521 tattgcttgt agctggcaat agttaatggg aggcgatatg cacgaaataa ccctctgcca
    32581 acgggcactg gaattgatcg aacagcaggc cgcaaaacac ggcgcaaaac gcgtaactgg
    32641 ggtctggctc aaaattggcg cattttcttg tgtcgaaacc agctctcttg ccttttgttt
    32701 tgatctggtt tgccgcggca gcgtggcgga aggttgtaaa ctgcacctcg aagaacaaga
    32761 ggccgaatgc tggtgtgaaa catgccaaca gtatgtgacg ctactgaccc agcgcgtccg
    32821 ccgctgtcca cagtgtcatg gtgacatgct acagattgtg gcagacgacg gtttacagat
    32881 tcggcggata gaaatagacc aggagtgagc gatgtgtaca acatgcggtt gcggtgaagg
    32941 caacctctat attgagggtg atgaacataa ccctcattcc gcgtttcgta gcgcgccatt
    33001 tgccccggcg gcacgcccga agatgaaaat caccggcatt aaagcgcctg aatttacccc
    33061 cagccagact gaagaaggcg acctgcatta cggtcatggc gaagcgggca ctcacgcgcc
    33121 gggcatgagc cagcgtcgga tgctggaagt cgaaattgac gtgctggaca aaaataaccg
    33181 tctggctgaa cgcaaccgcg cgcgctttgc tgcccgcaag caactggtgc tcaacctggt
    33241 ttccagccct ggttccggta aaaccaccct gctgacggaa actttaatgc gcctgaaaga
    33301 cagcgttccg tgcgcggtta ttgaaggtga ccagcaaacc gtgaacgatg ccgcacgcat
    33361 tcgcgctacc ggcaccccag cgattcaggt gaacaccggt aaaggctgcc atcttgacgc
    33421 acagatgatt gccgacgccg cgccgcgcct gccactggac gataacggta ttctgtttat
    33481 cgaaaacgtc ggtaacctcg tttgcccggc cagcttcgat ctcggtgaaa aacacaaagt
    33541 ggcggtgctt tccgttaccg aaggtgaaga caaaccgctg aaatatccgc acatgtttgc
    33601 cgccgcctcg ctgatgctgc tcaacaaagt tgacttgctg ccgtatctca atttcgacgt
    33661 tgagaagtgc atcgcctgcg cccgtgaagt caatccagaa attgaaatca tccttatttc
    33721 cgccaccagc ggcgaaggga tggaccagtg gctgaactgg ctggagacac agcgatgtgc
    33781 ataggcgttc ccggccagat ccgcactatt gacggcaacc aggcgaaagt cgacgtctgc
    33841 ggcattcagc gcgatgtcga tttaacgtta gtcggcagct gcgatgaaaa cggtcagcca
    33901 cgcgtgggcc agtgggtact ggttcacgtt ggctttgcca tgagcgtaat taatgaagcc
    33961 gaagcacgcg acactctcga cgccttacaa aacatgtttg acgttgagcc agatgtcggc
    34021 gcgctgttgt atggcgagga aaaataatgc gttttgttga tgaatatcgc gcgccggaac
    34081 aggtgatgca gttaattgag catctgcgcg aacgtgcttc acatctctct tacaccgccg
    34141 aacgccctct gcggattatg gaagtgtgtg gcggtcatac ccacgccatt tttaaattcg
    34201 gcctcgacca gttgctaccg gaaaacgttg agtttatcca cggtccgggt tgcccggtgt
    34261 gcgtactgcc aatgggtaga atcgacacct gcgtggagat tgccagccat ccggaagtca
    34321 tcttctgtac ctttggcgat gccatgcgcg taccagggaa acaggggtcg ctgttgcagg
    34381 caaaagcacg cggtgccgat gtgcgcatcg tttactcgcc aatggatgcg ttaaaactgg
    34441 cgcaggagaa tccaacccgt aaagtggtat tcttcggctt aggttttgaa accaccatgc
    34501 cgaccaccgc catcactctg caacaggcga aagcccgcga tgtgcagaat ttttacttct
    34561 tctgccagca cattacgctt atcccgacgc tgcgcagttt gctggaacag ccagataacg
    34621 gtatcgacgc gttcctcgcg ccgggccacg tcagtatggt tatcggcact gatgcctata
    34681 attttatcgc cagcgatttt catcgcccgc tggtggtcgc tggtttcgaa ccccttgatc
    34741 tactgcaagg cgtggtgatg ctggtggagc agaaaatagc ggcccacagc aaggtagaga
    34801 atcagtatcg tcgggtggta ccggatgccg gtaacctgct ggcgcaacag gcgattgccg
    34861 atgtgttctg tgtcaacggc gacagtgaat ggcgcggctt aggcgtgatt gaatcttctg
    34921 gcgtgcacct gacgccggat tatcaacgat tcgatgccga aacacatttc cgcccggcac
    34981 cgcagcaggt ctgcgatgac ccgcgtgcgc gttgtggcga agtcttgacg ggcaaatgta
    35041 agccgcatca atgcccgctg tttggtaaca cctgtaatcc tcaaaccgcg tttggtgcgc
    35101 tgatggtttc ctccgaagga gcgtgcgccg cgtggtatca gtatcgtcag caggagagtg
    35161 aagcgtgaat aatatccaac tcgcccacgg tagcggcggc caggcgatgc agcaattaat
    35221 caacagcctg tttatggaag cctttgccaa cccgtggctg gcagagcagg aagatcaggc
    35281 ccgtcttgat ttggcacagc tggtagcgga aggcgaccgt ctggcgttct caaccgacag
    35341 ttacgtcatt gacccgctgt tctttcctgg cggtaatatc ggcaaactgg cgatttgcgg
    35401 caccgccaat gacgttgcgg tcagtggcgc aattccgcgc tatctctcct gtggctttat
    35461 cctcgaagaa ggattgccga tggagacact gaaagccgta gtgaccagca tggcagaaac
    35521 cgcccgcgcg gcaggcattg ccatcgttac tggcgatacc aaagtggtac agcgcggcgc
    35581 ggcagataaa ctgtttatca acaccgctgg catgggcgca attccggcga atattcactg
    35641 gggcgcacag acgctaaccg caggcgatgt attgctggtt agcggtacac tcggcgacca
    35701 tggggcgact atccttaacc tgcgtgagca actagggctg gatggagaac tggtcagcga
    35761 ctgcgcggtg ctgacgccgc ttattcagac gctgcgtgac attcccggcg tgaaagcgct
    35821 gcgtgatgcc acccgtggtg gtgtaaacgc ggtggttcat gagttcgcgg cagcctgcgg
    35881 ttgtggtatt gaactttcag aagcggcact gccggttaaa cctgctgtgc gcggcgtttg
    35941 cgaactgctg ggactggacg cccttaactt tgccaacgaa ggcaaactgg tgatcgccgt
    36001 tgaacgcaac gcagcagagc aagtgctggc ggcgttacat tcccatccat tgggaaaaga
    36061 tgcggcgctg attggcgaag tagtagaacg taaaggtgtt cgtcttgccg gtctgtatgg
    36121 cgtgaaacga accctcgatc taccgcacgc cgaaccgctt ccgcgtatat gctaataaaa
    36181 ttctaaatct cctatagtta gtcaatgacc ttttgcaccg ctttgcggtg ctttcctgga
    36241 agaacaaaat gtcatataca ccgatgagtg atctcggaca gcaagggttg ttcgacatca
    36301 ctcggacact attgcagcag cccgatctgg cctcgctgtg tgaggctctt tcgcaactgg
    36361 taaagcgttc tgcgctcgcc gacaacgcgg ctattgtgtt gtggcaagcg cagactcaac
    36421 gtgcgtctta ttacgcatcg cgtgaaaaag acacccccat taaatatgaa gacgaaactg
    36481 ttctggcaca cggtccggta cgcagcattt tgtcgcgccc tgatacgctg cattgcagtt
    36541 acgaagaatt ttgtgaaacc tggccgcagc tggccacagg tgggctatac cccaaatttg
    36601 gtcactattg cctgatgcca ctggcggcgg aagggcatat ttttggtggc tgtgaattta
    36661 ttcgtcatga cgatcgcccc tggagcgaaa aagagttcaa tcgtctgcaa acatttacgc
    36721 agatcgtttc tgtcgtcacc gaacaaatcc agagccgcgt cgttaacaat gtcgactatg
    36781 agttgttatg ccgggaacgc gataacttcc gcatcctggt cgccatcact aacgcggtgc
    36841 tttcccgcct ggatatggac gaactggtca gcgaagtcgc caaagaaatc cactactact
    36901 tcgacattga cgatatcagc atcgtcttac gcagccaccg taaaaacaaa ctcaacatct
    36961 actccactca ctatcttgat aaacagcatc ccgctcacga acagagcgaa gtcgatgaag
    37021 ccggaaccct caccgaacgc gtcttcaaaa gtaaagagat gctgttgatt aatctccacg
    37081 agcgggacga tttagccccc tatgaacgca tgttgttcaa cacctggggc aaccagattc
    37141 aaaccttgtg cctgttaccg ctgatgtctg gcgacaccat gctgggcgtg ctaaaactgg
    37201 cgcaatgcga agagaaagtc tttaccacta ccaatctgag cttactgcgc cagattgccg
    37261 aacgtgtggc aatcgctgtc gataacgccc tcgcctatca ggaaattcat cgtctgaaag
    37321 aacggctggt tgatgaaaac ctcgccctga ccgagcagct caacaatgtt gatagtgaat
    37381 ttggcgagat tattggccgc agcgaagcca tgtacagcgt acttaaacaa gtggaaatgg
    37441 tggcgcaaag tgacagtacc gttctgatcc tcggtgaaac tggtacaggt aaagagctga
    37501 ttgcccgcgc gatccataat ctcagtgggc gtaataatcg ccgtatggtc aaaatgaact
    37561 gcgcggcgat gcctgccgga ttgctggaaa gcgatctatt tggtcatgag cgtggggctt
    37621 ttaccggtgc cagcgctcag cgcattggtc gttttgaact ggcggataaa agctcactat
    37681 tcctcgatga agtgggcgat atgccgctgg agttacagcc gaagttgctg cgtgtattac
    37741 aggaacagga gtttgaacgt ctcggcagca acaaaatcat tcagacggac gtacgtttaa
    37801 ttgccgcgac taaccgcgat ctgaaaaaaa tggtcgccga ccgagagttc cgtagcgatc
    37861 tctattaccg tctgaacgta ttcccgatcc acctgccgcc actacgcgag cgtccggaag
    37921 atattccgct gctggcgaaa gcctttacct tcaaaattgc ccgtcgtctg gggcgcaata
    37981 tcgacagcat tcctgccgag acgctgcgca tcctgagcaa catggaatgg ccgggcaacg
    38041 tgcgtgagct ggaaaacgtc attgagcgcg cggtattgct aacacgcggc aacgtgctgc
    38101 agctgtcatt gccagatatt gctttaccag aacctgaaac gccgcctgcc gcaacggttg
    38161 tcgcccagga gggcgaagat gaatatcagt tgattgttcg cgtgctgaaa gaaacaaacg
    38221 gcgtggttgc cgggcctaaa ggcgctgcgc aacgtctggg gctgaaacgc acgactctgc
    38281 tgtcacggat gaagcgactg ggaattgata aatcggcatt gatttaacag atgtggttat
    38341 actcgatcaa cattcaatga aaggaggcag tatggcagtt tcagcaagaa atcaattaac
    38401 cggaacagtt agcacagtag caatgggcgc tgtgaatgat gaagtagaac tgacgctggc
    38461 aggtggtgca aagctggtgg caattgtgac tcacagcagc cagcaagcgc tgggtctggc
    38521 gaaagggaaa gaagcgattg ccctgattaa agccccgtgg gtcacgctgg caacggaaga
    38581 ctgtggactg aaattctctg ctcgcaacca gtttgctggc agcgtgtcga caatcaccga
    38641 aggcgcagtg aacgccactg tccatatcaa aacggatgcg ggttttgaaa ttgtggcagt
    38701 ggtaacaaat gaaagtcagg atgaaatgaa actcaccact ggctcgcgcg ttattgcatt
    38761 gatcaaagca tcagccatcc tgattgccac caaagcgtaa acgccttatt tgccggagag
    38821 tatggctctc cggcagattt atttctttgg acgatatttt tctggcaact ccggcaccgg
    38881 acgcttgtca tcgatgagat gacgcacggt taagatcgga tgacgccaca gcattctcgg
    38941 cccggcccaa cgcataatct gtttcatctc ttcacgcttt gcaggctggt aacagtgcac
    39001 cggacactgc ttacaggctg gtttctcttc accgaacaca catttatcca gccgcttttg
    39061 cgcgtaagca aacaacgcct cgtaatgctc cggctctgct gacgcctgcg ggcatttcgc
    39121 ttgataaaga tcgatcattt ttttaatcgt cagtttttca cgagagatac gcttgccgga
    39181 catgctgcct ccatactctt aaggtgcatt tatattacaa cttaatttta aaggggacta
    39241 tggctctgaa ggaggaggtt tcttcaaccg acgaatttgg cgatcttgtt tcatcgtcat
    39301 cacgcaaaag ctgcaaagca gcatttttcg cggaaccgac atcaagaact cacctttcgc
    39361 gtcttcccct gaaatgatta actccggtat catgtgcgcc ttatgtgatt acaacgaaaa
    39421 taaaaaccat cacactacat ttaacatcag ggagccggac ttaaccccat gagaacaata
    39481 gaaaatttcg acgcccatac gcctatgatg cagcagtatc tcaagctgaa agcccagcat
    39541 cccgagatcc tgctgtttta ccggatgggc gacttttatg aactgtttta tgacgacgca
    39601 aaacgggcgt cgcaactgct ggatatttca ctgaccaaac gcggtgcttc agcgggagag
    39661 ccaatcccga tggcggggat tccctaccat gcggtggaaa actacctcgc caaactggtg
    39721 aatcagggcg aatcggttgc tatttgtgaa cagattggcg atccggcgac cagcaaaggg
    39781 ccggtcgagc gcaaagttgt ccgtatcgtt acgccgggca ccatcagcga tgaagcgctg
    39841 ttgcaggaac gtcaggacaa cctgctggcg gctatctggc aggacagcaa ggggttcggc
    39901 tacgccacgc tggatatcag ctccgggcgt tttcgcctga gcgaaccggc ggaccgcgaa
    39961 acgatggcgg cagaactgca acgtacgaat ccagcggaat tactgtatgc ggaagatttc
    40021 gccgagatgt cgctgattga aggtcgccgc ggcctgcgtc gtcgcccgct gtgggagttt
    40081 gaaatcgaca ccgctcgcca gcaattgaac ctgcaatttg gcacccgaga tctggtcggt
    40141 tttggtgtgg agaacgcacc acgcggactt tgtgctgccg gttgtctgtt gcagtatgcg
    40201 aaagataccc aacgcacgac cctgccgcat attcgttcta tcactatgga acgtcagcag
    40261 gacagcatca ttatggatgc cgcgacgcgt cgtaacctgg aaattactca gaacctggcc
    40321 ggcggtgcgg aaaatacgct ggcttcagtg ctcgactgta ctgtaacgcc gatgggtagt
    40381 cgtatgctga aacgctggct gcatatgcca gtgcgcgata cccgcgtgtt gcttgagcgc
    40441 cagcaaacta ttggcgcatt gcaggatttc accgccgagc tgcagccggt actgcgtcag
    40501 gtcggcgacc tggaacgtat tctggcgcgt ctggctttac gaactgctcg tccacgcgat
    40561 ctggcccgta tgcgtcacgc tttccagcaa ctgccggagc tgcgcgcgca actggaaggt
    40621 gtcgatagcg ccccggtaca agcactgcgt gagaagatgg gcgagtttgc cgaactgcgc
    40681 gacctgctgg agcgagcaat catcgacacg ccgccggtgc tggtacgcga cggtggtgtt
    40741 attgcgactg gttataacga agaactggat gaatggcgcg cgctggctga cggcgcgacc
    40801 gattatctgg agcgtctgga agtccgcgag cgtgaacgta ctggcctgga caccctgaaa
    40861 gtcggcttta acgcggttca tggttattac attcagatca gccgtggaca aagccatctg
    40921 gcacccatca attacatgcg tcgccagacg ctgaaaaacg ccgagcgcta cattattcca
    40981 gagctaaaag agtacgaaga caaagttctc acttcaaaag gcaaagcact ggcactggaa
    41041 aaacagcttt atgaagagtt gttcgacctg ctgttgccgc atctggaagc gttgcaacag
    41101 agcgcgagcg cgctggcgga actcgacgtg ctggtgaacc tggcggaacg ggcctatacg
    41161 ctgaactaca cctgcccgac ctttattgat aaaccgggta ttcgcatcac cgaaggccgc
    41221 catccggtgg ttgaacaggt gctgaatgag ccgtttatcg ccaacccgct gaacctgtcg
    41281 ccgcagcgtc gcatgttgat cattaccggt ccgaatatgg gcggtaaaag tacctatatg
    41341 cgccagaccg cactgattgc gctgatggcc tacatcggca gctatgtacc ggcacaaaaa
    41401 gtcgagattg gacctatcga tcgcatcttt acccgcgtag gggcagcgga tgatctggca
    41461 tccgggcgtt caacctttat ggtggaaatg accgaaaccg ccaatatttt acataacgcc
    41521 accgagtaca gtctggtgct gatggatgag attgggcgtg gaacgtccac ttacgatggt
    41581 ctgtcgctgg cgtgggcgtg cgcggaaaat ctggcgaata agattaaagc gttgacgttg
    41641 tttgctaccc actatttcga gctgacccag ttaccggaga aaatggaagg cgtcgccaac
    41701 gtgcatctcg atgcactgga gcacggcgac accattgcct ttatgcacag cgtgcaggat
    41761 ggcgcagcga gcaaaagcta cggcctggcg gttgcagctc tggccggcgt gccaaaagag
    41821 gttattaagc gcgcacggca aaaactgcgt gagctggaaa gcatttcgcc gaacgccgcc
    41881 gctacgcaag tggatggtac gcaaatgtct ttgctgtcag tgccagaaga aacgtcgcct
    41941 gcggtcgaag cactggaaaa tctcgacccg gattcactga ctccgcgtca ggcgctggag
    42001 tggatttatc gcttgaagag tctggtgtaa taacaattcc cgatagtctt ttgctatcgg
    42061 gaatattaac gacaactgac gaatcaaata aaaataccct gtataatagg aaatcttatt
    42121 ttacagggta aaaccatgcc atctacacgc taccaaaaaa tcaatgccca tcactatcac
    42181 catatatggg ccgttggtga tattcatggt gactatcaat tattacaatc ccgcttacat
    42241 caactctctt tttgtcctga aaccgacttg cttatttctg tcggcgataa catcgatcgt
    42301 gggcccgaga gtcttaacgt gctgcgcctg ttaaaccagc cctggtttat ctccgtcaaa
    42361 ggcaaccacg aagcgatggc gcttgaggca ttcgaaactg gcgatggaaa tatgtggctt
    42421 gccagcggtg gtgactggtt tttcgattta aatgattcag agcaacaaga ggcaatagat
    42481 ctgttgctga aattccatca ccttccacat attattgaaa tcactaacga caccataaaa
    42541 tatgtcatcg cacatgcaga ttatccgggg aaggagtatc agtttggcaa agaaatagcg
    42601 gagagcgaat tactctggcc tgttgatcgt gtgcagaaat cacttaatag cgaattacaa
    42661 aaaataaacg gcgctgatta ttttatattt ggacatatga tgtttgataa cattcagacg
    42721 ttcgcaaacc agatttatat tgataccgga tcgccgaaaa gcgggcggct gtcattttat
    42781 aaaataaagt agtctcatgc ttcttctgtg aagcatgagt aacccggcgt tattgcaggt
    42841 cattattcat ttttcgctac cagcaaagag agatccagct tcgccagcgc gcggctggca
    42901 ctctccggca gaccatcgtc agtaatgatc tgatcaaact cgcttaatgg taacgccagc
    42961 catgtcgcca cctgtccata tttcgtcgca tcacagacca aaactcgctg gcggctggca
    43021 ctggcaatcg cccgtttcac cgtgacttta tcttctgctg gcgtagagat cccccgcaca
    43081 ctccatgacg atgcagaaat aaaagcctga tcaatcatca ggctacgcag catggtcgca
    43141 gcggcttccc caacacagga acggttttcc cgacacactg cgccgccagt gtgaataatt
    43201 gtgcaattac tgttgtcgag caagtagtcc gcaataacga agtcgttagt aaccactgtg
    43261 agtggctcca tgtgagtcag atgctgtgca atcgctaacg tggtcgttcc cgcatccaga
    43321 tagatacaac gtcccggctg aacaagactt gccgccagct tgccaatagc cgctttttgc
    43381 gtcattgcca gcgcagtttt tacctgatga gaaggttcat gcgccacccg tcccggagac
    43441 tggacgcccc cggacaccaa cacaacggct ccctgctgct ccagtttttg taaatcccga
    43501 cgaatggtca tatgtgacac attcattctg tccgttagtt cagcaatact aacaatgcct
    43561 ttttcagcta ccatctcaag gatgatttgg cgacgctcta cgggtatcaa cttttgctcc
    43621 ttcctttgtc ctgctgacat tctacgctat ttgcctgcga aacgtgcgcg gcgcaactaa
    43681 cgcttagttc acataaaata acatacaatg ttaatttttg tgaatcagct caccatatcg
    43741 ttatctttcc gcgcttatat tcacaacatc aaacaaaata tcacttaaat taacaaggag
    43801 agcagatgaa aacgggatcg gagtttcatg tcggtatcgt tggcttaggg tcaatgggaa
    43861 tgggagcagc actgtcatgt gtccgcgcag gtctttctac ctggggcgca gacctgaaca
    43921 gcaatgcctg cgctacgttg aaagaggcag gtgcttgcgg agtttctgat aacgccgcga
    43981 cgtttgccga aaaactggac gctctgctgg tgctggtggt caatgcggcc caggttaaac
    44041 aggtgctgtt tggtgaaaca ggcgttgcac aacatctgaa acccggtacg gcagtaatgg
    44101 tttcttccac tatcgccagt gctgatgcgc aagaaattgc taccgctctg gctggattcg
    44161 atctggaaat gctggatgcg ccagtttctg gtggtgcagt aaaagccgct aacggtgaaa
    44221 tgactgtcat ggcctccggt agcgatattg cctttgaacg actagcaccc gtgctggaag
    44281 ccgttgccgg aaaagtttat cgcataggtg cagaaccggg actaggttcg accgtaaaaa
    44341 ttattcacca gttgttagcg ggcgtacata ttgctgccgg agccgaagcg atggcacttg
    44401 cagcccgtgc ggggatcccg ctggatgtga tgtatgacgt cgtgaccaat gccgccggaa
    44461 attcatggat gttcgaaaac cggatgcgtc atgtggtgga tggcgattac accccgcatt
    44521 cagccgtcga tatttttgtt aaggatcttg gtctggttgc cgataccgcc aaagccctgc
    44581 acttcccgct gccattggcc tcaacagcat tgaatatgtt caccagcgcc agtaacgcgg
    44641 gttacgggaa agaagacgat agcgcagtta tcaagatttt ctctggcatc accctaccgg
    44701 gagcgaaatc atgatcaaga ttggcgttat cgccgatgat tttaccggcg cgacggatat
    44761 cgccagtttt ctggtggaaa acggtctacc aacggtacaa attaacggtg ttccaacagg
    44821 taaaatgccg gaagcaatcg acgcactggt gatcagcctg aaaacgcgct cctgtccggt
    44881 ggttgaagcc acacagcaat cgctggcggc tctgagctgg ttgcaacagc aaggttgcaa
    44941 acagatctat ttcaaatact gctctacttt cgacagttcg gcgaaaggta atatcggccc
    45001 ggttaccgat gcattaatgg atgctctcga cacgccgttt acggtcttct ctccggccct
    45061 gccggtcaac ggacgtacgg tttatcaggg gtatttgttc gtaatgaatc aactgctggc
    45121 cgaatccggg atgcgccatc acccggtaaa tcccatgacc gacagctatc ttccccgtct
    45181 ggttgaagcg caatccacag ggcgctgcgg cgtcgtttcg gcacatgttt tcgaacaagg
    45241 tgtggatgcc gttcgtcagg agctggctcg cttacagcaa gagggctacc gctacgcggt
    45301 gcttgatgcg ctgaccgaac accatctgga aattcaggga gaagccttgc gcgatacccc
    45361 actggtaacg ggcggttctg gtctggcgat tggccttgcc cggcagtggg cgcaagaaaa
    45421 cggtaaccag gctcgcgaag cagggcgtcc gctcgctggg cgcggcgtag tgctctccgg
    45481 ttcatgctct caaatgacca accgccaggt ggcacattac cgtcaaattg caccagcccg
    45541 tgaggttgat gtggcacgct gcctctcaac cgaaactctg gccgcttatg cacacgagct
    45601 ggcagagtgg gttctgggcc aggaaagtgt acttgctcca ctggtttttg ccaccgccag
    45661 cactgacgca ttggcagcaa ttcaacagca atacggtgca caaaaagcca gtcaggcagt
    45721 ggaaacactg ttttctcaac tagcggcgcg gttagcagcg gaaggcgtga cacgctttat
    45781 tgtcgcaggc ggtgagacct ccggcgtagt cacacagagc ctgggcataa aaggttttca
    45841 tattggccca accatttccc ccggcgtgcc gtgggtaaac gcactggata agcctgtctc
    45901 actcgccctt aaatctggca acttcggtga tgaagccttt ttttcacgag cccaaagaga
    45961 gtttttatca tgagcgattt cgcaaaagta gagcagtctt tgcgagagga gatgacgcgg
    46021 attgccagtt cattctttca gcgcggatat gcaactggtt cggctggcaa tctgtcgctg
    46081 cttttacctg acgggaattt actggcgaca ccgacaggtt catgcctggg caatctcgat
    46141 ccgcagcggc tttccaaagt caccgcggat ggcgaatggt taagtggtga caaaccctcg
    46201 aaagaggtgc tctttcatct ggcgctgtat cgcaacaatc cgcgctgtaa agcggtagtg
    46261 catttgcaca gtacgtggtc gacggcgctt tcctgcctgg aagggctgga cagcaataac
    46321 gttattcggc cgttcacacc atacgtggtg atgcggatgg gaaacgtccc gctggtgccc
    46381 tattaccgac cgggcgataa acgcatcgca caggatctgg cggaactggc agcagacaat
    46441 caggcttttt tactggcaaa tcatggccca gtggtttgcg gtgaaagcct gcaagaagcc
    46501 gccaacaata tggaagagct agaggaaacg gcaaagctga tttttattct cggtgaccgc
    46561 ccgatccgtt atctgaccgc aggtgaaatt gcggaattaa ggagttaatg caatgcctcg
    46621 ttttgcagct aatttatcca tgatgttcac cgaagtgcct tttattgaac gcttcgccgc
    46681 agcgcgaaaa gccgggttcg atgctgtgga gtttctgttt ccctatgact actcccccca
    46741 gcaaatccag cagcaactgg cgcaaaacca cctgacgctg gcgctgttca ataccgctcc
    46801 cggagatatt aatgcgggag aatggggatt atccgccctt cccggacgtg agcacgaagc
    46861 acgtgcagac atcgacctgg cactggaata tgcattggcg ctcaattgtg aacaagtcca
    46921 tgtgatggca ggcgtcgtgc ccgctggcga agatgctgaa cggtaccggg cagtatttat
    46981 tgataatctc cgctacgctg ccgaccactt tgcgccacac ggcaagcgaa tattggttga
    47041 agcactcagc cccggcgtta agcctcatta tctcttctcc agccagtatc aggcactggc
    47101 tattgttgaa gaggttgcgc gagataacgt ttttattcaa ctagatactt tccatgcgca
    47161 aaaagtagat ggcaacctga cgcatttaat ccgcgactac gccgggaaat atgcgcacgt
    47221 acaaattgcc ggactacctg atcgacatga accggacgat ggagaaatca actatccgtg
    47281 gctgttccgc ctgttcgatg aagtgggata tcagggctgg atcggttgtg aatacaaacc
    47341 tcgtggcctc accgaagaag gacttggctg gtttgacgcc tggcgctaat tcgttaaatc
    47401 cctaattaca acgtacccat acatcccccc atctacggat ggggtaggtt aactataact
    47461 ttcagacagg gtttcccatg tccacaatta cattgttatg cattgcgtta actggcgtaa
    47521 tcatgctgtt gctgctggtc atcaaggcaa aggtacaacc attcgttgct ctgctcctcg
    47581 tcagcctgtt agtcgcactt gcggcgggta taccggcggg cgaagtgggt aaagtgatga
    47641 tcgccgggat gggcggtgtg cttggttccg tcactattat tattggtctg ggcgctatgc
    47701 tggggcgtat gatcgaacac tctggtggtg cagagtcact ggctaattat ttcagtcgca
    47761 agttaggcga caaacgaact atcgctgcgc tgactctggc agcgttcttc ctcggtattc
    47821 ccgtcttctt tgatgtcggc tttattattc ttgcgccaat catttacggt tttgccaagg
    47881 ttgccaaaat atcaccactc aaatttggtc tgcctgtcgc tgggatcatg ctcactgttc
    47941 acgtggcggt accgcctcat ccaggccctg tcgccgcagc ggggttactc cacgcagaca
    48001 tcggctggct aaccatcatc ggtattgcga tttctattcc cgtagggatt gttggctact
    48061 ttgcagcgaa aataatcaat aagcgccaat atgcgatgtc ggtcgaggta ctggaacaga
    48121 tgcaactggc tccggccagt gaggaaggcg cgacaaaatt aagcgataaa ataaatccac
    48181 cgggcgtcgc gctggtcacc tcgctaattg ttattcctat cgcgattatc atggcgggta
    48241 cggtttccgc aacactgatg ccgccttcgc atcccctgct tggtacgcta cagctgatcg
    48301 gctcaccaat ggtagctcta atgattgcgc tggtgctggc attctggtta ttggctttac
    48361 gtcgcggctg gagcttacaa cacaccagcg acattatggg ctcagccctt cccactgcgg
    48421 cggtagtgat tttggttact ggtgctggag gggtatttgg caaagtgctg gtggaatcgg
    48481 gcgtcggtaa agcccttgcc aacatgttac aaatgattga cctgcctctg ttaccagccg
    48541 catttattat ttcactggcg ctgcgtgcat cgcaggggtc agcaaccgta gcaatcctga
    48601 caaccggcgg gttactctca gaagcggtga tgggattgaa tccaattcag tgcgtattgg
    48661 tgacgctggc agcctgcttt ggtgggcttg gcgcgtcaca tattaatgac tcagggttct
    48721 ggattgtgac caaatatctg gggttgtcgg tagcagacgg gctgaaaacc tggacagtgt
    48781 taacgaccat tctcggtttt accggattct taattacctg gtgcatatgg gcggtaattt
    48841 gaccttaata aaaggtccga tgggcatcgg accttttatt tagcgcttac cttccgccag
    48901 cagcggcggg atgctcggca ccatcggcgc gtcatcaata tctttctgcg tcatgcggaa
    48961 cgcttcggga taatgttcgc ggctggtgcg gcgtagcggt tcggtatcgc gccaggtata
    49021 gaggcaatgc tggcactggt agaccatcca gacatctttt accggcgatt tcgccatcac
    49081 ttcaatctgt tcatcggcac aacgtggaca aatcatcttg ttctccttat tgacgtgcgg
    49141 ccagcatagc ggtcagtttt tcagcccagg ctttggtttc cggtaagtcc accaccggct
    49201 ggctgtagtg accacggttg tccggggcga caggcgtggt ggcgtcgata atcagcttgt
    49261 cggtgatccc cgccggactt gagccagggt cgagttccag cacggacata ttcggcaact
    49321 gtaccaaatc ccctgccggg ttcactttcg aggagagcgc ccacatcacc tgcggcaggt
    49381 tgaacggatc aacgtcttca tcgaccataa tcaccatctt cacgtagccc agaccgtgcg
    49441 gcgtggtcat cgcacgcagg cccaccgcgc gggcaaagcc gccgtagcgt tttttggtgg
    49501 agataatcgc cagcaggccg tgggtgtaca tggcgtttac cgcctgcact tccgggaact
    49561 cggctttcag ttgttgatac agcggcacgc aggtggcagg ccccatcagg tagtcgattt
    49621 cggtccacgg cataccaaga tagagcgatt cgaaaatcgg tttggtacgg taagagactt
    49681 tatcgatacg caccacggtc atgttacgcc cgccggagta gtggccggta aactcaccga
    49741 acggcccttc aatctcacgt ttacgacttt cgataacccc ttcgaggatc acttccgagc
    49801 cccacggcac atcaaaaccg gtcagcgggg cggtggcgat cgggtacggg ctttcgcgca
    49861 gcgcgcctgc catttcgtac tcagactgat cgtatttcag cggcgtggcc cccattaggg
    49921 tgatgatcgg atcgttaccg agtgtgatgg caatcggcag atcttcaccg cgctcttctg
    49981 ctttatgcag atgcagggcg atatcgtgca tcggcaccgg ttgcaggccg agcttacgct
    50041 tgcccttcac ttccatgcgg tagatgccga cgttctgctt gccgaagtta tccgggtcga
    50101 gcggatcgcg ggaaaccacg cacgctttat cgagatagaa accgccatca ccgtcgttta
    50161 aacgaaacag cggcagaata ttgaacaggt tgatctcgtc gccatcgacg gtgttctgcg
    50221 cccaggctgg attggcgcgg cgctccgggg caatggggaa gttatcccag cggcggataa
    50281 actcatcaat ctgtttttta accggggtgt ttggcggcag gccgagggaa atcgcgtggt
    50341 tctgccagga accgatggtg ttcattgcca cgcgggcatc gataaagccg cgaatattat
    50401 caaaccaaag cgcgggcgcg ccgtcgccga tacgcccggt ggcgttggct gcggctgcca
    50461 gatccggttc cgcgttcacc tcttcgctga ttttcagtaa ctggccgtgg tcatcaagcg
    50521 cctgtaaaaa gctgcgtaaa tcatcaaatg ccattattca ttctcctgag aaaaattccg
    50581 ggcctgcggc aatccttgcc agcgcctggc gtgaggatgt tcgaggccaa attgatccag
    50641 cacgcgggct accacgtggt ggacaatgtc atctaccgtt tcgggatggt tataaaaggc
    50701 aggcatcggc ggcaccatcg ccacgcccat gcgcgaaagt gcgagcatat tttcgagatg
    50761 gattgtgcta agcggcattt cacgcggcac cagcaccagt ttgcggcctt ctttgagcac
    50821 gacgtccgcc gcgcgcccca ccaggccatc ggcgtaacca gcgcggatac cggcgagcgt
    50881 tttcatactg catggaataa cgatcatgcc gtcggtacga aaggagccgg aagagatagt
    50941 cgccgcctga tccgccgggt tatggctgaa gtctgcgagg gcggcaacat cgcgagcgct
    51001 gtaaggcgtt tccagttcaa tcgtggtttt cgcccacttc gacatcacta gatgagtctc
    51061 gacattcggc atctcccgca gcgcttgcag taatgccaca ccaagaggcg caccggtagc
    51121 ccctgtcatc ccgacgatca gtttcattgc ttactctctt aattgttcgt atacgaacgt
    51181 tattattaac atactccttc atttccacag cggcaaggat tgaaaagtaa caacatcttc
    51241 ttcgtcactg aaaaaccgct atgatagcga cagagaatta gcccggagtt tacatggcgt
    51301 tacgaaataa agcgttccat cagttacgac agctttttca gcagcataca gctcgctggc
    51361 agcacgagtt acctgacctg accaaaccac agtacgcggt gatgcgcgcc attgctgata
    51421 agcctggtat cgaacaggtg gcactgatag aagcagcagt cagcaccaaa gcaacgctgg
    51481 cagaaatgtt ggcaagaatg gaaaatcgtg gcttagtcag acgtgaacat gacgccgctg
    51541 acaagcggcg acgctttgtc tggctgactg ccgaaggaga aaaggtactt gctgcggcga
    51601 tccccattgg tgacagtgtg gatgaggaat ttttggggcg tttgagtgca gaagagcagg
    51661 agctgtttat gcagttggtg cgcaagatga tgagcaaata ggatgctgat tgccgggtag
    51721 gacgctgacg agtcttatcc aggcgacaaa acacaccgac ttcccaaaca aaagaaaaag
    51781 gccagcctcg cttgagactg gcctttctga cagatgctta cttattcgcg gaacagcgct
    51841 tcgatattca gcccctgcgt ttgcaggatt tcgcgcaaac ggcgcaggcc ttcaacctga
    51901 atctggcgaa cacgttcacg ggtgaggcca atttcacgac ctacatcttc cagtgttgcc
    51961 gcttcgtacc ccagcaaacc gaatcgacgt gccagtactt cacgctgttt ggcgttcagc
    52021 tcgaacagcc atttgacgat gctctgcttc atatcgtcat cttgcgtggt atcttccgga
    52081 ccgttctctt tttcatcggc caggatgtcc agcaacgctt tttcggaatc accacccagc
    52141 ggggtgtcta ccgaggtaat gcgctcgtta agacgaagca tacggctgac gtcatcaact
    52201 ggcttatcca gttgctctgc gatctcttcc gcacttggtt cgtggtccag tttatgggac
    52261 aactcgcgtg cggttcgcag gtaaacgttc agctccttta cgatgtgaat aggcaaacga
    52321 atagtacggg tttggttcat aatcgcccgt tcgatcgtct ggcgaatcca ccaggttgcg
    52381 tatgttgaga agcggaaacc acgttccggg tcaaacttct ctaccgcgcg gatcagcccc
    52441 aggttgccct cttcgatcag gtccagcaac gccagaccac gattgccata acggcgggca
    52501 atttttacca ccagacgcaa gttactctcg atcatccggc ggcgggaggc aacatctcca
    52561 cgcagtgcgc gacgcgcaaa ataaacttct tcttcggccg ttaacagtgg tgaataacca
    52621 atctcaccaa ggtaaagctg agtcgcgtcc agcacacgct gtgtggctcc ctgcgataac
    52681 agttcctctt cggccaaatc gttatcactg ggttcctctt ctactaaggc cttttcgtca
    52741 aaaacctcaa ctccgttctc atcaaattcc gcatcttcat ttaaatcatg aactttcagc
    52801 gtattctgac tcataaggtg gctcctaccc gtgatccctt gacggaacat tcaagcaaaa
    52861 gcctggttcc gccgatttat cgctgcggca aataacgcag cgggtttacg gatttcccct
    52921 tgtaacgaat ttcaaaatgc aagcgtgttg aactggttcc ggtgctaccc atggttgcta
    52981 ttttttgccc cgccttcact tcttgttgtt cccggaccag cattgtgtcg ttatgggcgt
    53041 aggcactcag gtaatcatca ttatgtttga tgataatcag attaccgtag ccgcgcagcg
    53101 cgttaccggc ataaacaacg cggccatctg cggtcgcgat aattgcctgt cctttgctgc
    53161 ctgcgatatc aatccccttg ttgcccccct cagaagcgcc aaaggtttcg atcactttgc
    53221 cctcagtcgg ccagcgccag gtggagatag gcgtactggt tgatgtactg ctgacagtcg
    53281 gctcggttgt gcttgctgtt ggtaccgtta caggcgctgt gaccgtggtc gcagttggct
    53341 tgttgttcgg caacattttg ttagcactct gttcacccga agactcagaa tacgtaattg
    53401 tcggttgcga cgcaacagca acggtggaat tttgtgcagg cttgatcaca actccttgct
    53461 ctgctgcgtc ggcctgggta atggcatttc cgccagtgat tggcgtaccg gaagcattac
    53521 ccacctgtaa ggtctgaccg acgttcagcg cgtatggtgc ctgaatattg ttgcgctgag
    53581 caaggtcacg gaaatcgttg ccagtaatcc aggcgatata gaaaagtgtg tcgccttttt
    53641 tcacggtata ggtactgccg ctataactgc ctttcggaat gttcccatac tgacggttgt
    53701 agacgatgcg tccgttttcc atctgtaccg gctgctgagc tactggctgt actggctgga
    53761 tttgcggttg ttgagtagcc tgaatttgtg gctgctgtac cggctgaatt tgcggttgct
    53821 gcgctgtaga cgtcgtcccc attttcggcg gcggcgtaat caacatacca gaattggtat
    53881 ttgcaggcgc attgccatta acggagctga ccggtgccgg tggatttgaa gtgtcagaac
    53941 agcctgccag ccatagcgaa accagtgaca aagccgcaat gcggcgaacg gtgaattttg
    54001 ggcttcccgc gctcatttat cccccaggaa aaattggtta ataaccagtg acataattac
    54061 cgtgcaaggc accatactga acactggaaa agatgttcac gatacgctga cctgcggcaa
    54121 aataaccagg aaaaatccag gtatttcctc acgttttaag ccagctcacc cttcactaaa
    54181 gggacaaagc gcacggcctc cacggtatcg ataataaatt cgcctccccg acgacgcacc
    54241 cgtttcaaat actggtgctc ctcccctacg ggtaagacga gaattccgcc ttcgtccagc
    54301 tgcgtcatta gcgcagttgg aatttccggc ggtgccgccg taacaatgat agcgtcaaac
    54361 ggcgcacgtg cctgccaacc ttgccatcca tcgccatgac gggttgaaac attatgtaaa
    54421 tcaagatttt tcaggcggcg acgcgcctgc cactgcaagc ctttaatccg ttcaaccgag
    54481 caaacatgct ggacaagatg cgccaggatt gccgtttgat atcccgaacc ggtgccaatt
    54541 tccagcaccc gcgactgcgg cgtcagttcg agtaattcgg tcattcgcgc caccatatac
    54601 ggctgcgaaa ttgtctgccc ctgacctatc ggcaaggcga tattgtccca ggctttttgt
    54661 tcaaacgctt catcaacgaa tttttcacgc ggcacggcgg caagtgcatt cagcaccagc
    54721 tcatcctgaa taccttgcgc acgtaattga tccagaagtg cttgtacgcg tctgcttacc
    54781 attgcgtgcc aactcccacg ctgtttaacc agtctgaaac cacatcttgc gcgctatgcg
    54841 cagttaaatc cacatgcagc ggcgtgatgg aaacatagcc ctcatctacc gcagcaaaat
    54901 cggtccccgg accggcatca catttaccgc ccggcgggcc aatccagtac agcgtattgc
    54961 cgcgcggatc ttgctgcggg atcacctgat ctgccggatg tcgtgtaccg cagcgcgtca
    55021 cgcgaatacc tttgatttga tccaatggta aatccggaac gttaatatta agaatacgtc
    55081 cggtgcgcag cggctcttta cacagtgcgc gcaaaattga acaggttacc gccgcggcag
    55141 tgtcgtaatg tttatgcccg tcaagcgaga cggccagcgc cggaaaacct aaatgacggc
    55201 cttccatcgc ggcggctacc gtaccggaat aaataacatc atcccccaga ttcggcccgg
    55261 cgttaattcc ggacacaaca atgtccgggc gcggacgcat cagagcattc acgccaagat
    55321 agacgcaatc ggtcggggtt cccatttgca cagcaatatc accattttca aaggtaaacg
    55381 tgcgcaggga ggattccagt gtcagagaat ttgaagcgcc gctgcggtta cgatcggggg
    55441 cgaccacctg aacgtcagca aactcacgca aggctttcgc cagcgtttgt ataccgggtg
    55501 catgtacccc gtcatcatta ctcagcaata tgcgcataat cacctgttgt gttgataagt
    55561 tccctgacaa cgctggttgc aaaacttccc gccggaagcc agaaacggat ttctacggtg
    55621 acgtcatccc accagttcca gcttaatttt tgcggataca gcagcatcgc tctgcgcgcg
    55681 gcttcaactt tttcacgcac cagtaaagtt tgtaattcag tttcttcggc gacagctgct
    55741 tgttcgaatg ccagcgcttc acgctgagtt ccccattcgc cactgcccgg caatgcggcg
    55801 gttatcatca gctctttatc gttgacgcga cgctgtaatt ccgccagttc ttcggtggtt
    55861 gcaacaaacc agctaccacg tccggctaat tgtagcgcat cgccgtcaac aacttgatta
    55921 acgtctgctt ttttgaggcg ctcagcaaca atctgattaa acaacgcact gcgggctgcc
    55981 gacaaccaaa aactccgttt attgcgatcg cgcaccggag tattggtttg cgcccaacgc
    56041 agcgcgccct gcaagttgct accgccaatc ccaaaacgtt gggcaccgaa gtagttcggt
    56101 acacctttta cgcaaatatc gatcagacgt tgttcaacgt catcgcgatt gctcacttcg
    56161 cgtagtacca gggtaaaggc gttacctttc agcgccccta aacgcagctt gcgcttgtgc
    56221 cgcgcatact ccagcacctg gcagccttcc agttgaaagg cgctcagatc gggcatctct
    56281 ttgcccggca cgcgagcgca taaccactgt tccgtaacag catgtttatc tttttgccca
    56341 gcgaagctga cttcacgggc atgaattttc aggaatttcg ccagtgcatc cgccacaaaa
    56401 cgggtattgc agccgttttt gaggattcta accagaatat gctcaccttc accatcaggc
    56461 tcaaagccca aatcttccac caccacaaag tcttccggat tggctttcag cagcccggtg
    56521 ccttgcggtt taccgtggag gtaagtgaga ttatcaaact caatcatttt gttgccttaa
    56581 tgagtagcgc caccgcttca caggcaatcc cttccccacg tccggtaaat cccagttttt
    56641 ccgtagtagt ggctttcacg ttaacatcat ccatatggca gccgagatct tcggcaataa
    56701 acacgcgcat ttgtggaatg tgcggcaaca tcttcggtgc ctgagcgatg atagtgacat
    56761 cgacgttgcc gagggcataa cctttcgcct gaatacgacg ccaggcttcg cgcaacagct
    56821 cgcggctgtc ggcaccttta aatgccggat cggtatccgg gaacagcttg cctatatctc
    56881 ccagcgccgc cgcgccaagc aatgcatcgg tcaacgcatg gagcgccacg tcgccatcag
    56941 aatgcgccag cagccctttt tcgtaaggaa tgcgtacgcc accaatgata attgggcctt
    57001 caccgccaaa ggcgtgtacg tcaaaaccgt gtccaattcg cattatgtat tctcctgatg
    57061 gatggttcgg gtgaggtaaa attcggccag cgccaaatcc tccgggcgcg ttaccttgat
    57121 gttatccgcg cggccttcaa caagttgcgg atgaaaaccg caatattcca gcgccgaggc
    57181 ctcatcggta atggtagcgc cttcatttag agcgcgcgtc aggcagtcat gtaacagctc
    57241 acgagggaaa aattgcggcg tcagcgcgtg ccataagccg ttgcgatcaa cggtatgagc
    57301 aatggcattt ttgcccggtt cggcacgttt catcgtatcg cgcaccggcg cagcgaggat
    57361 cccccccgtg cggctggttt cgctcaacgc caacaatcgc gcgaggtcat cctgatgcag
    57421 acaaggacga gcggcgtcat gcaccaatac ccactgcgcg tcgccagcgg ctttcagacc
    57481 tgccagcacg gaatcggcac gctcttcacc accatcaaca accgtgattc gtggatgatt
    57541 cgccagagga agttgtgcaa agcggctatc gccaggactt atggcaatga cgacacgttt
    57601 caccctggga tgcgccagca gcgcatacac cgagtgttca agaatggttt gattaccgat
    57661 tgagagatat tgcttaggac attccgtttg cattcggcgg ccaaatccgg ccgccggaac
    57721 cacggcgcaa acatccaaat gagtggttgc catgttaatt cccgggctga tttatcgatt
    57781 attttgcccc gcagactgtg cgcgctttga cgcgtcaggc accagacgat aaaacgtttc
    57841 gcccggcctg gtcatgctga gttcattacg cgcacgctct tcgagcgcct cctggccgcc
    57901 attgagatcg tcaatttcgg caaaaagctg atcgtttcgc gctttaagtt tcgcgtttgt
    57961 agcttgctgt gccgccacat cattattgac gcgagtatag tcatgtatac cgttcttacc
    58021 gaaccacagc gaatactgta gccagaccag aatagccagc aacagcagcg ttagtttacc
    58081 catcctgccc cctgaaaaac ggcatcttca tcccatgcat ccgaagacga ctctacatcc
    58141 tctgttgggg ataccgcgac aacgcgggca aatgtaccac atttgtccat tgttacgtat
    58201 acccagggcg tgcagaacat aatctcatta ttagttacgg attgaattat gaacagaata
    58261 gagaggaaag aacaattaac ccagtagcca cataaacagc gcaccaaaca taatgccaac
    58321 cgtcgccaga gtgaaaacaa tactgtagcg tagctttccg tccatcaatg aatgcagtgc
    58381 aatccccacc actacagcga cgggcatcag cgccagaaag aaaggccagg tgtagataaa
    58441 aaagagcagc gtgttagagc cataaatcaa catcggaatt gccaacgcgc acagccacga
    58501 taaaaaacca accacagcgc ctggcagcga ccatgtggtg tcttcaacct cagtaaggct
    58561 gtcgttattt gttaatgtaa tgttgtggct gttacgcata tctgatcctg ttactttgac
    58621 gaaccgggca tggaaacccg gcggtgtctc aggatctgat aatatcgttc tgtctcaaca
    58681 gatctaataa ttgctgtacc aaatttgtta ctaattgttc accattgaga tgaatttctg
    58741 ccgattcagg cgcttcgtaa acggaatcta ttcccgtaaa gttgcgcagt tcaccggcac
    58801 gcgctttctt atataaacct tttggatcgc gggcttcgca aatcgccagc ggcgtatcga
    58861 caaacacttc gataaagcgc ccttctccta cgcgttcgcg aaccatctgg cgttcggcgc
    58921 ggtgtggcga gataaatgcg gtcagcacca ccagtccggc ttcaaccatc aaattcgcca
    58981 cttcaccaac ccgacggata ttctctttac ggtcggcatc gctaaaaccg agatcgctgc
    59041 ataatccgtg gcgaacgttg tcgccatcca gcagatacgt actgacgcca agtttatgta
    59101 acgcctcctc cagcgccccc gcgacggttg atttaccgga accagagagg ccggtaaacc
    59161 acagcacaac accacgatga ccgtggtgct gttcgcgttg ttgcggagtt actgggtggc
    59221 tatgccagac gacgttttcg tcatgcagcg ccattattta tctcccagta aatcgcgcgc
    59281 accccaatgc gggaaatggc ggcgaaccag ggcattcaat tccagttcga atgcactgaa
    59341 ttcagatggc gcagcagttg cctggctaac tggttcgtgc accataccgg cacctacggt
    59401 cacattactc aggcgatcga taaaaatcag cccaccggta accgggtttt gctgataacg
    59461 atctaacacc agtggctcgt caaaggtgag atctaccagg ccgatgccgt tcagcggcag
    59521 gttttcaact tcgcgttggg taaggttatt gatatcaacc tgataacgaa tgccatcaac
    59581 acgagcacgc gtcttcttac cggcaatttt gatgtcataa ctctggcccg gggaaagcgg
    59641 ctgttccgcc atccatacca catccaccga cgcgctctgc acagccggta acgcttcgtc
    59701 tgccgccagc agcagatcgc cacggctgat gtcgatctca tccgtcagca ccagggtgat
    59761 cgcttctccg gcaaaggctt cttcgcgatc gccatcaaaa gtcacgatcc gcgcgacgtt
    59821 tgattccaca ccagagggca gcactttgac acgttgcccg acttccacgc gaccggatgc
    59881 cagcgttccg gcgtagccac gaaaatcgag attcggacgg ttaacgtact gcaccgggaa
    59941 gcgcattggc tgagcatcca ccactcgctg gatctccacg gtttccagca cttctagcag
    60001 tgtcggaccg ctgtaccacg gcatactttc actttgcgaa gccacgttgt cgccttccag
    60061 tgcggagagc ggcacaaagc ggatatccag attacccggc agctgcccgg caaaagtcag
    60121 ataatcttca cgaatacggg tgaacgtctc ttcactgtaa tccaccagat ccattttgtt
    60181 gatcgccacg accagatgtt tgatccccaa cagtgtggag ataaaactat gacgacgggt
    60241 ttgatcgagc acgcctttac gggcatcgat cagtaagatc gccagttcac atgtcgatgc
    60301 gccagtcgcc atattgcggg tgtactgctc gtgccctggg gtgtcggcga taataaattt
    60361 acgcttctcg gtagagaaat agcggtaggc cacatcaata gtgatgccct gttcgcgctc
    60421 agcttgcagg ccgtccacca gcagtgccag atccagcttt tcgccctggg tgccgtgacg
    60481 cttactgtcg ttatgcagcg atgagagctg atcttcgtag atttggcggg tatcgtgcag
    60541 caggcgacca atcagggtac ttttgccgtc atcgacgcta ccacaggtca gaaaacgcag
    60601 caggctttta tgttgttgcg caatcatcca tgcttcgacg ccgccttcat tggcgatttg
    60661 ttgtgcaagt gcggtgttca tcttaaaaat acccctgacg ttttttcagc tccatagacc
    60721 ccgcctggtc gcggtcaatt acgcggccct gacgttcact ggtggtggaa accagcatct
    60781 cttcgatgat ctccggcagt gtttgcgcat ttgactccac cgcgccggtc agcggccaac
    60841 agcccagtgt gcggaaacgc accatccgtt ttttaatcac ttcgcccggt tgcaggtcga
    60901 tacggttgtc atcaatcatc atcaacatac cgtcgcgttc cagaaccgga cgttccgcag
    60961 caagatacag cggaacaatg tcgatatttt ccagccagat gtattgccag atatcctgct
    61021 cggtccagtt agagagcggg aagacgcgga tgctttcgcc tttgttaatt tgcccgttgt
    61081 agttgtgcca cagctccggg cgctgatttt tcggatccca gcgatggaag cggtcacgga
    61141 aagagtagat tcgctcttta gcgcgggatt tctcttcgtc acggcgcgca ccaccgaagg
    61201 cggcatcaaa accgtatttg ttcagcgcct gtttcaggcc ttcagttttc ataatatcgg
    61261 tatgtttcgc gctgccgtgt acgaatggat taatccccat cgccacgcct tccgggtttt
    61321 tatgcaccag cagctcgcag ccgtaggctt tcgccgtacg atcgcggaac tcatacatct
    61381 cgcggaattt ccagccggta tcgacatgca gcaacgggaa aggcagcgta cctggataaa
    61441 acgccttgcg cgccagatgc agcatgacgc tggaatcttt accgatagag tagagcatca
    61501 ccggatttga gaattctgca gccacctcgc gaataatgtg gatgctttcc gcctccagtt
    61561 gccgcaggtg ggtaagtcgt ttttgatcca taaccgttcc tttgcaatac cgctattttc
    61621 ttgccatcag atgtttcgac tatagggagc gtaagagaac gaatgaaatt accaattaga
    61681 atgagtagtt ccttaacgga ataacgattt ggcaaagcta atatcaaaaa gtgcttaagg
    61741 caccggattt cgggcgttta ggaagatttg aaattgtttt agcgcagcgg cagtttcata
    61801 ctatgacggt aaaaaaattt gcatggtatt taaggactca ctatgttttc cgcattgcgc
    61861 caccgtaccg ctgccctggc gctcggcgta tgctttatcc tccccgtaca cgcctcgtca
    61921 cctaaacctg gcgattttgc taatacccag gcacgacata ttgctacttt ctttccggga
    61981 cgcatgaccg gaactcctgc agaaatgtta tctgccgatt atattcgcca acagtttcag
    62041 caaatgggtt atcgcagtga tattcggaca tttaatagtc ggtatattta taccgcccgc
    62101 gataatcgca agagctggca taacgtgacg ggaagtacgg tgattgccgc tcatgaaggc
    62161 aaagctccgc agcagatcat cattatggcg catctggata cttacgctcc gctgagcgat
    62221 gctgacgccg atgccaatct cggcgggctg acgttacaag gaatagatga taacgccgca
    62281 ggtttaggtg tcatgctgga actggcagaa cgcctgaaaa atacgcctac cgagtatggt
    62341 attcgatttg tggcgaccag tggtgaagag gaagggaaat taggtgctga gaatttactc
    62401 aagcggatga gtgacaccga aaagaaaaat acgctgctgg tgattaatct cgataactta
    62461 attgttggcg ataaattgta tttcaatagt ggtgtaaaaa cccctgaagc agtaaggaaa
    62521 ttaacgcgcg acagggcgct ggcaattgcg cgtagtcatg gaattgccgc aacgaccaat
    62581 ccgggtttga ataaaaatta tccgaaaggc actgggtgtt gtaatgacgc agaaatattc
    62641 gacaaagccg gtattgctgt actttcggtg gaagcgacta actggaatct tgggaataag
    62701 gatggttatc agcaacgcgc aaaaacagcc gcattccctg cgggaaatag ctggcatgac
    62761 gtaagactgg ataatcaaca acatattgat aaggcgcttc ctggaagaat agaacgtcgc
    62821 tgccgtgatg ttatgcggat aatgctacct ctggtgaagg agttggcgaa ggcgtcttga
    62881 tgggtttgaa aatgggagtt gggagttcta ccgcaggggc ggggaattct aagtgatatc
    62941 catcatcgca tccagtgcgc ccggtttatc cccgctgacg cggggaacac acatgaatgt
    63001 cggttcagac cgtgttttta cccggtttat ccccgctggc gcggggaaca cttgccaacc
    63061 gccaaagact ggaaaaccaa tctcggttta tccccgctgg cgcggggaac acgaactggc
    63121 gttgagtgat aaatttgacg gaatcggttt atccccgctg gcgcggggaa ctcttcagtt
    63181 tcacactcgg taatgccgtt atttacggtt tatccccgct ggcgcgggga actcctcggg
    63241 gccagcctcc cccattaacc cggcaccggt ttatccccgc tggcgcgggg aactctctaa
    63301 aagtatacat ttgttcttaa agcatttttt cccataaaaa caacccacca accttaatgt
    63361 aacatttcct tattattaaa gatcagctaa ttctttgttt ttaaacaggt aaaaaagaga
    63421 ccaaccttaa accatccaaa tctaccggag tacgcctgtt taccccaaat gtctggaact
    63481 caaatcccga ttccgtattc gtagcccatg ccatcaccac attgccttct tccgccagtc
    63541 cagatatttg ttcccagatc atttcacgga ctcttgcgga tacatcacct acatacaccc
    63601 ctgcacgtac ctccaacaac cagatggcta atctgcctcg taagcgcgaa ggtacatttt
    63661 cagtgaccac gaccaacata ctcatctcag ctactccgat gtccggcatc tcccagagaa
    63721 acgggaagtg gaatagcaac aggctgcgca tcttcaggtg gggccggcgg ctgtatttcc
    63781 ccagcagcaa gcacgtcctc tataagcgga atcaatttga ctaatgtttt actactgcga
    63841 aaaatatccc tgcacgccaa acggacttcc cggtccggct caccaggaat acgacgcgct
    63901 atctcaaaag ctttcggtac aacagtgtca aatttaatga tgtccgcgat atcgtaaaca
    63961 aaggaaagag gctttcctgt atgcacaaac ccaatagctg gtgcataacc agctgcaagt
    64021 atcgccgctt cagttacgcc atataaacaa gaagttgcag cgctaatgca ttggttgacc
    64081 gtatcgccct tttcccagtc tttcggatcg taacgacgtc cattccattt caccccgtat
    64141 tgcttcgcca gaagtacata ggttgctcgc acacgacttc cttctatacc tctgagttgc
    64201 tctacggagc gccgggcagg cgcaggttct ccaaaccgaa gttcaaacat tttacgtacg
    64261 accttcagac gcaaatcttc atccagagca agttttgcct gatagagcag cttatctgaa
    64321 cgcgcaccac caggctgacc agaagcataa acacgaacgc ccgcttcccc cacccatacc
    64381 aataatgttc caacttgcgc agccaggcgt acagctgcat gcgaaacccg tgtaccaggt
    64441 tccaacatga tgcaggcaac cgagccaaca ggaatatgag tgcggatccc tgtcttgtcg
    64501 ataagtacaa acgcgccatc tattacatcg atctgcccat attgcagaaa gatcatggag
    64561 acgcgatctt tgagtggaat gggattaagg ggaagccagg tcattttatt acacctcaat
    64621 cacagtggag ccaaagatag caagccacat cccatcgatt tagctggccc aataccttgc
    64681 tgtacaagat ctattaacgc tggcgcgtcg ttgatggtga tcaccccttc aaagcaaacc
    64741 gtttggatct ttccactttt accatcacca gaaaaatact gtggccgttc cgatatggga
    64801 tgcacatctt caacgcgcgc cgcattgccc aatttacgtt gcaaccacgc gatttgttct
    64861 gcttctttta ttaacggaac ccgacagcgt ttaatattcc ctttactgtc caggcgcttt
    64921 tgattgtcga gaatagtttt gatcggattt gcccgaagcc gaaaatagag tggaacacca
    64981 acctgaagtt gaaattcaac ctgtttagtt ttaatgactg tcgcaacggc agtagaaaca
    65041 ggcatttgcg ctgactgcaa taaaacatga cagccttctg gtgtgtttcg cttctcaaca
    65101 tgaaaaagaa aatcacgagc agcatccggt ctgtttggaa ataaatgcca taatccctgg
    65161 tgaagttggt aaagatccct gctccaggcc ctggcaatga tgactttact gagatacatc
    65221 catacctcct ttaatcacat accattctcg ggaagcaaat tgtcgaggta aggtgatcat
    65281 cggttcgtcg cgcaccgtaa attttaaatg atgcccgtca actgattcct cactataaat
    65341 atcgccgcca acgggctcat aatttaatag cgccttctga ggatccgacg cctgacatgt
    65401 cccgagaaaa agcggttgtg ttagcggaca actcctccgc cccagatacg gtgtataccg
    65461 aggctttaat actgcttttt caagttctga cataaccatc gttgcctgtg gtgttaacca
    65521 gatagcgacg gtaaaggagg catcacataa atattcgcgc catgtttgaa tcgtttcatg
    65581 acttttcaaa ccacggtaat cttctcgcgc tccaaggact gtatggtaat cacgcaaccc
    65641 cgttacagac acacgacgat cgtcaagaat gagttcatcg cagcgcactg caaattgcac
    65701 actctctgat aacgcctgta atgaagaagt atcatcacgt tggatcccaa gacaagcccc
    65761 gagtagccct aataacccgc ttcgggtcgg aaatcttccg gtaggtcgcg ttccttcaaa
    65821 ggtcggctgc ccccaggctt gcattggccc agcaagccgc aagatcaaat aagatctcat
    65881 gttcacgcct cgccattatt acgaacccag gattttaact gttctaaagt aggcatttgt
    65941 ttaacttgag cagtaattgg gtctacatca gataagctga attgcgcagc agctccgttc
    66001 agaccatatc cattggcaac gcgatcccaa tattgattaa acgcctgtat agacggttgc
    66061 aaaaagccat ctttcgcttt aaccgctttt tcaaaagcat ttgccataga aagtggcata
    66121 tcggagaaat taaccattac catatccgca gggttaaaag cggcataagt acgctgtttt
    66181 gctccaggga cctctgttgc cagcatatga acaacatggg ttgcaatttc cagagcctgc
    66241 tccctggagg caccacctaa attttcctga agttgagcga ggttaatgtt ggcataacga
    66301 taaaaaacac ccgatgaaaa ttcctgagtt cccagatgtg cagaaccttg ttcctgtaaa
    66361 tcatctacag cggtgaacca gtcaatatca gaatcaacct gatgagtagt gatcgcatgc
    66421 gcaatggaca ttgcaccatc aacttttccc aactcactca tcatgccgct ggttgccatt
    66481 cttccactaa gcgcaatatc aacaccctgc tgtaaattca cacgtatggc ggcaatatct
    66541 tccttaagaa ctttgagcag ctttttatca tccagattat cagcctctgc ttttgcaacc
    66601 tgctcacaga accaggctat ttctcccaca acctagggag taaccgcatc ggcagaaatc
    66661 ttttcggctt catcaactaa tttaccggag agcagcgcta atgtcttatc gatgattttt
    66721 tggtcaaaac gttcaccaag tttttgccga agaacatcac gtaattgtgc aagatgaatt
    66781 gttctgagac tggattcacc aatattttgt gcgtaataat cacttttacg catcgcatgt
    66841 ttaaggcttt gacttgaaat tcttactcgt cttttcccgc cgaaaatagc gtctttctgc
    66901 atgttcatat cgtcgcggtt cagacatgaa gggctgtgag agatcagaac atgaatattg
    66961 ataaagttag acatagaaag gtttccttac gcatttttgt ttgtggtcaa tacaaaatct
    67021 tccagaagtt gctggcgttc gcgctttccc caccaggtca acatcctggc cattaatggc
    67081 cagtcaagta cgggttcggc gtgagtaagt aatcgacgta actggaccat atcggctgtt
    67141 ctgtcagccc gaattaattg aaagatacgg cgctcgttaa ttcttccact attggctaaa
    67201 gctcttccca acgagatacc tgttgtttgc tccgtttttt tgtcctgatg tcggatgaca
    67261 ttctttcctg cgctcaggca aaacaccatg cgcaaaagag cctgctggtg acgtgggttt
    67321 tcccaaccaa aagattgcac cagcctataa aacgcaggga tatcgcgtaa ttcatcaggt
    67381 tctgaaacac gtctaatttg cgcacatgct ccattatcca gttgttgcca ggctcgatat
    67441 aaagccattg catcaatttc atcagccatt tgatggccct ccttgaggtt ttaactcccg
    67501 taaatgtttg tatagcgtgg cgcgggcaag cgctaatgtg cttattaatt taggatgatg
    67561 tgcataggga gctacagatt gattaaatag catttcacac aattgatgaa gtttgtctcg
    67621 taaatcagct attacctcat cagcttggga aaaattaata ttcgccaata catcgggaat
    67681 taataattca ctctgtcgat agaaatgcct ttctgcagtc tcatgaacag aaactccggc
    67741 ccctttgaag tctttatttt taaacccttc tgcaaaggta tataacgcct tgcgtaaggc
    67801 tgttttatat cccaaaccaa cagtcactat ttcgtttatc acattgccgt attgttgcca
    67861 cccctgatta aacatcaaca catcatgacg ccgttcaaga atagatgctt gattattacg
    67921 atatcccccc ataatcaatt caagaggact ttgcggcgca atatttctga attgattcac
    67981 aaccgccgcc acgcgatttc cattttcatt ttgaataatc ttatctacca caactcggct
    68041 gatttgtgtc catgatggtg cggaggtggt gaaagcaaga aatttttcct caacctcccc
    68101 tttcttgact gttaccagac aaggggaatg cggatggggc catagcccat taactgtaaa
    68161 ggtaaatttt tccttaagaa aaccggtata acgcaaattg ctttcctgac cacagcaaga
    68221 acatttacca atcccaatgg gatcgcatag ttcaatatgc gctggttgcc agaataaacc
    68281 acggacaaac ccaattgaag aagcaggcac cgattcattc ggcttgacag gcttaaccca
    68341 ggtaggttgg ttttccgtat gtaattcatt gggaaattgt ttttgaagac gaggtattgt
    68401 gaggacattg agtaataccg tcgagcgaag atcgatccca catacgaagg tggttatagg
    68461 tgttcctcca cgtaaaccgc ttttaaagcc accaccaaaa ccaggtgcct gattcgcctg
    68521 gttgaataac gcaatcgcag tgcatccacc acataatgct tcaccctgcc ccggttgatt
    68581 gacaaatgca caattcgtcg cgccgcttac cccagccaac agtttttcca ttggagtcac
    68641 atcatttgct ttgacacctt tggtctgcat aaagggatgt tctgcgtgat taaggtagaa
    68701 catatctatc cacggcgcga tgagttgttg aaactcatct tcagtgagcg gattcattat
    68761 gcgatgtcga aattcaacgt catcttttgc cggggcgata atttgcccaa tgcaaaccag
    68821 cagtgctaaa gtggccagtt ccatatcgtc acggggcaaa cttaatcgcc actgatctct
    68881 actgcagtat agcgattgca gatttatgat ttggactttc cccccgtttc gcgggcgtac
    68941 agggatccag ttatcaataa gcaaattcat ttgttctcct tcatatgctc cgacatttct
    69001 cctgcatttc tatgcgtcgg cacacttccg ttatcggtaa aaacaaagaa aaaatacggc
    69061 gtaattaatt caataatcac attc
//
LOCUS       DAZGHF010000007        66439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGHF010000007 DAZGHF010000000
VERSION     DAZGHF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.007"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(184..1131)
                     /gene="dusC"
                     /locus_tag="ACHK3A_000535"
     CDS             complement(184..1131)
                     /gene="dusC"
                     /locus_tag="ACHK3A_000535"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO8738672.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPAGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(1193..2386)
                     /locus_tag="ACHK3A_000536"
     CDS             complement(1193..2386)
                     /locus_tag="ACHK3A_000536"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO8738673.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARQGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEIARQRAVFTDHITMMDAVNAEEVVRIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWIGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPCCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(2401..3045)
                     /gene="maiA"
                     /locus_tag="ACHK3A_000537"
     CDS             complement(2401..3045)
                     /gene="maiA"
                     /locus_tag="ACHK3A_000537"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO8738674.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLESHYPQVPLMPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(3053..3754)
                     /locus_tag="ACHK3A_000538"
     CDS             complement(3053..3754)
                     /locus_tag="ACHK3A_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO8738675.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAIVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEN
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKASETGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGSVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(3770..4798)
                     /gene="gtdA"
                     /locus_tag="ACHK3A_000539"
     CDS             complement(3770..4798)
                     /gene="gtdA"
                     /locus_tag="ACHK3A_000539"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO8738676.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTSMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVTIGNETFSFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(4810..6168)
                     /locus_tag="ACHK3A_000540"
     CDS             complement(4810..6168)
                     /locus_tag="ACHK3A_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8738677.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAIIRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAVCIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTQLITVVRQSTSVPDSLPHAGVV
                     NE"
     gene            6295..7203
                     /locus_tag="ACHK3A_000541"
     CDS             6295..7203
                     /locus_tag="ACHK3A_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8738678.1"
                     /translation="MANWAQKLKLHHLQMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGLTLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLKEMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYRVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            7334..7732
                     /gene="yohJ"
                     /locus_tag="ACHK3A_000542"
     CDS             7334..7732
                     /gene="yohJ"
                     /locus_tag="ACHK3A_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO8738679.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            7729..8424
                     /gene="yohK"
                     /locus_tag="ACHK3A_000543"
     CDS             7729..8424
                     /gene="yohK"
                     /locus_tag="ACHK3A_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO8738680.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            8554..9438
                     /gene="cdd"
                     /locus_tag="ACHK3A_000544"
     CDS             8554..9438
                     /gene="cdd"
                     /locus_tag="ACHK3A_000544"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO8738681.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALVAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            9588..10307
                     /gene="sanA"
                     /locus_tag="ACHK3A_000545"
     CDS             9588..10307
                     /gene="sanA"
                     /locus_tag="ACHK3A_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO8738682.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            10310..10549
                     /gene="yeiS"
                     /locus_tag="ACHK3A_000546"
     CDS             10310..10549
                     /gene="yeiS"
                     /locus_tag="ACHK3A_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO8738683.1"
                     /translation="MDVQQFFVVAVFFLIPVFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            10868..12106
                     /gene="preT"
                     /locus_tag="ACHK3A_000547"
     CDS             10868..12106
                     /gene="preT"
                     /locus_tag="ACHK3A_000547"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO8738684.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLKGACSC"
     gene            12100..13335
                     /gene="preA"
                     /locus_tag="ACHK3A_000548"
     CDS             12100..13335
                     /gene="preA"
                     /locus_tag="ACHK3A_000548"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO8738685.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEDNLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(13406..14416)
                     /gene="mglC"
                     /locus_tag="ACHK3A_000549"
     CDS             complement(13406..14416)
                     /gene="mglC"
                     /locus_tag="ACHK3A_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO8738686.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(14432..15952)
                     /gene="mglA"
                     /locus_tag="ACHK3A_000550"
     CDS             complement(14432..15952)
                     /gene="mglA"
                     /locus_tag="ACHK3A_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO8738687.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(16013..17011)
                     /gene="mglB"
                     /locus_tag="ACHK3A_000551"
     CDS             complement(16013..17011)
                     /gene="mglB"
                     /locus_tag="ACHK3A_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO8738688.1"
                     /translation="MNKKVLTLFAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(17291..18331)
                     /gene="galS"
                     /locus_tag="ACHK3A_000552"
     CDS             complement(17291..18331)
                     /gene="galS"
                     /locus_tag="ACHK3A_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO8738689.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNLAM"
     gene            complement(18473..19630)
                     /gene="yeiB"
                     /locus_tag="ACHK3A_000553"
     CDS             complement(18473..19630)
                     /gene="yeiB"
                     /locus_tag="ACHK3A_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO8738690.1"
                     /translation="MGRNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPARSKTSR"
     gene            complement(19647..20315)
                     /gene="folE"
                     /locus_tag="ACHK3A_000554"
     CDS             complement(19647..20315)
                     /gene="folE"
                     /locus_tag="ACHK3A_000554"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO8738691.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            20573..21409
                     /gene="yeiG"
                     /locus_tag="ACHK3A_000555"
     CDS             20573..21409
                     /gene="yeiG"
                     /locus_tag="ACHK3A_000555"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO8738692.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(21441..23420)
                     /gene="cirA"
                     /locus_tag="ACHK3A_000556"
                     /pseudo
     CDS             complement(21441..23420)
                     /gene="cirA"
                     /locus_tag="ACHK3A_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(23713..25182)
                     /gene="lysP"
                     /locus_tag="ACHK3A_000557"
     CDS             complement(23713..25182)
                     /gene="lysP"
                     /locus_tag="ACHK3A_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO8738693.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAVS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(25387..26268)
                     /gene="yieE"
                     /locus_tag="ACHK3A_000558"
     CDS             complement(25387..26268)
                     /gene="yieE"
                     /locus_tag="ACHK3A_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO8738694.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            26367..27416
                     /gene="yeiH"
                     /locus_tag="ACHK3A_000559"
     CDS             26367..27416
                     /gene="yeiH"
                     /locus_tag="ACHK3A_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO8738695.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            27490..28347
                     /gene="nfo"
                     /locus_tag="ACHK3A_000560"
     CDS             27490..28347
                     /gene="nfo"
                     /locus_tag="ACHK3A_000560"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO8738696.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTSAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            28350..29438
                     /gene="yeiI"
                     /locus_tag="ACHK3A_000561"
     CDS             28350..29438
                     /gene="yeiI"
                     /locus_tag="ACHK3A_000561"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO8738697.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(29494..30744)
                     /gene="nupX"
                     /locus_tag="ACHK3A_000562"
     CDS             complement(29494..30744)
                     /gene="nupX"
                     /locus_tag="ACHK3A_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO8738698.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKSFINRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(30844..31785)
                     /gene="rihB"
                     /locus_tag="ACHK3A_000563"
     CDS             complement(30844..31785)
                     /gene="rihB"
                     /locus_tag="ACHK3A_000563"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO8738699.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            31915..32613
                     /gene="yeiL"
                     /locus_tag="ACHK3A_000564"
     CDS             31915..32613
                     /gene="yeiL"
                     /locus_tag="ACHK3A_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO8738700.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(32681..33931)
                     /gene="psuT"
                     /locus_tag="ACHK3A_000565"
     CDS             complement(32681..33931)
                     /gene="psuT"
                     /locus_tag="ACHK3A_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO8738701.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(34025..34963)
                     /gene="psuG"
                     /locus_tag="ACHK3A_000566"
     CDS             complement(34025..34963)
                     /gene="psuG"
                     /locus_tag="ACHK3A_000566"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO8738702.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELAHTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(34951..35892)
                     /gene="psuK"
                     /locus_tag="ACHK3A_000567"
     CDS             complement(34951..35892)
                     /gene="psuK"
                     /locus_tag="ACHK3A_000567"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO8738703.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(36316..38007)
                     /gene="fruA"
                     /locus_tag="ACHK3A_000568"
     CDS             complement(36316..38007)
                     /gene="fruA"
                     /locus_tag="ACHK3A_000568"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO8738704.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVATSG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKEGAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYISFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(38024..38962)
                     /gene="fruK"
                     /locus_tag="ACHK3A_000569"
     CDS             complement(38024..38962)
                     /gene="fruK"
                     /locus_tag="ACHK3A_000569"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO8738705.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFDVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(38962..40092)
                     /gene="fruB"
                     /locus_tag="ACHK3A_000570"
     CDS             complement(38962..40092)
                     /gene="fruB"
                     /locus_tag="ACHK3A_000570"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO8738706.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            40460..41641
                     /gene="setB"
                     /locus_tag="ACHK3A_000571"
     CDS             40460..41641
                     /gene="setB"
                     /locus_tag="ACHK3A_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO8738707.1"
                     /translation="MHNSPAVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVTVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(41638..41892)
                     /gene="yeiW"
                     /locus_tag="ACHK3A_000572"
     CDS             complement(41638..41892)
                     /gene="yeiW"
                     /locus_tag="ACHK3A_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO8738708.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            42047..42619
                     /gene="yeiP"
                     /locus_tag="ACHK3A_000573"
     CDS             42047..42619
                     /gene="yeiP"
                     /locus_tag="ACHK3A_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO8738709.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            42842..44308
                     /gene="yeiQ"
                     /locus_tag="ACHK3A_000574"
     CDS             42842..44308
                     /gene="yeiQ"
                     /locus_tag="ACHK3A_000574"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO8738710.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQSGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARALMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLARE
                     TDWSLLALGVAGWMRYVSGVDDAGNTIDVRDPLSDKIRDLVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            44426..45412
                     /gene="yeiR"
                     /locus_tag="ACHK3A_000575"
     CDS             44426..45412
                     /gene="yeiR"
                     /locus_tag="ACHK3A_000575"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO8738711.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHGVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVMRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            45451..46164
                     /gene="lpxT"
                     /locus_tag="ACHK3A_000576"
     CDS             45451..46164
                     /gene="lpxT"
                     /locus_tag="ACHK3A_000576"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO8738712.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            46576..47142
                     /gene="mepS"
                     /locus_tag="ACHK3A_000577"
     CDS             46576..47142
                     /gene="mepS"
                     /locus_tag="ACHK3A_000577"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO8738713.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            47323..48879
                     /gene="pdeN"
                     /locus_tag="ACHK3A_000578"
     CDS             47323..48879
                     /gene="pdeN"
                     /locus_tag="ACHK3A_000578"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO8738714.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFTDLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLKELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            48961..50775
                     /gene="yejA"
                     /locus_tag="ACHK3A_000579"
     CDS             48961..50775
                     /gene="yejA"
                     /locus_tag="ACHK3A_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO8738715.1"
                     /translation="MIVRILLLFIALFTFGAQAQTIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNAGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRLEND
                     AKNWVTRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            50776..51870
                     /gene="yejB"
                     /locus_tag="ACHK3A_000580"
     CDS             50776..51870
                     /gene="yejB"
                     /locus_tag="ACHK3A_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO8738716.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNTGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            51870..52895
                     /gene="yejE"
                     /locus_tag="ACHK3A_000581"
     CDS             51870..52895
                     /gene="yejE"
                     /locus_tag="ACHK3A_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8738717.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            52897..54486
                     /gene="yejF"
                     /locus_tag="ACHK3A_000582"
     CDS             52897..54486
                     /gene="yejF"
                     /locus_tag="ACHK3A_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO8738718.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIVFDGKPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(54490..54834)
                     /gene="yejG"
                     /locus_tag="ACHK3A_000583"
     CDS             complement(54490..54834)
                     /gene="yejG"
                     /locus_tag="ACHK3A_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO8738719.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(55168..56358)
                     /gene="bcr"
                     /locus_tag="ACHK3A_000584"
     CDS             complement(55168..56358)
                     /gene="bcr"
                     /locus_tag="ACHK3A_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO8738720.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(56386..57081)
                     /gene="rsuA"
                     /locus_tag="ACHK3A_000585"
     CDS             complement(56386..57081)
                     /gene="rsuA"
                     /locus_tag="ACHK3A_000585"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO8738721.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            57231..58991
                     /gene="radD"
                     /locus_tag="ACHK3A_000586"
     CDS             57231..58991
                     /gene="radD"
                     /locus_tag="ACHK3A_000586"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO8738722.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAVTRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            59116..59400
                     /gene="rplY"
                     /locus_tag="ACHK3A_000587"
     CDS             59116..59400
                     /gene="rplY"
                     /locus_tag="ACHK3A_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO8738723.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(59539..60546)
                     /gene="yejK"
                     /locus_tag="ACHK3A_000588"
     CDS             complement(59539..60546)
                     /gene="yejK"
                     /locus_tag="ACHK3A_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO8738724.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            60728..60955
                     /gene="yejL"
                     /locus_tag="ACHK3A_000589"
     CDS             60728..60955
                     /gene="yejL"
                     /locus_tag="ACHK3A_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO8738725.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            60975..62735
                     /gene="yejM"
                     /locus_tag="ACHK3A_000590"
     CDS             60975..62735
                     /gene="yejM"
                     /locus_tag="ACHK3A_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO8738726.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            62810..62886
                     /locus_tag="ACHK3A_000591"
     tRNA            62810..62886
                     /locus_tag="ACHK3A_000591"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62844..62846,aa:Pro,seq:ggg)
     gene            complement(63012..63771)
                     /locus_tag="ACHK3A_000592"
                     /pseudo
     CDS             complement(63012..63771)
                     /locus_tag="ACHK3A_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            64091..64738
                     /gene="narP"
                     /locus_tag="ACHK3A_000593"
     CDS             64091..64738
                     /gene="narP"
                     /locus_tag="ACHK3A_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO8738727.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(64773..65825)
                     /gene="ccmH"
                     /locus_tag="ACHK3A_000594"
     CDS             complement(64773..65825)
                     /gene="ccmH"
                     /locus_tag="ACHK3A_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO8738728.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKAAPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(65822..66379)
                     /gene="dsbE"
                     /locus_tag="ACHK3A_000595"
     CDS             complement(65822..66379)
                     /gene="dsbE"
                     /locus_tag="ACHK3A_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO8738729.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKL
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
BASE COUNT        16195 a        17499 c        16500 g        16245 t
ORIGIN      
        1 ttatccctta cggctaccgg gtcgggcgcg aggtaacatc cctgtaaaac gcgccctcag
       61 cccacatcca tgtgggctgc cccggccttc agggaacgcc tcggcgattt tgacgccacc
      121 aaacaaccgt gcggcctatt gataaagagc taacacattg tcaaaaaaca tcactatggt
      181 ttcttagagt ttctcgatat caattgcctg aatagccctt gcaatatcag gggaattatt
      241 caacacccga acatgctgaa ataattccgt tgcttcatcg tattctttac gcaaataact
      301 caaccactgt ttaatccgcg caacgtgata taacccggta tcgccctgct tttccagacg
      361 ggtatatttt tgcagcaaag caactacctc cggccacggc attcgcggtt cgttatattt
      421 taccacccgg ctcaggttgg gaatattgag cgccccgcga ccaatcatca ctgcgtcgca
      481 tccgctgatc gtcatgcatt gttgcgcact ctgccagtcc cagatttcac cgttggcaat
      541 cacaggaata ttcaaccgct ggcgaatctc gccaatcgcc tgccagtcaa tatgctccgc
      601 gcggtaaccc tgctcttttg tccgcccatg tactaccagc tccgtagcgc cagcctgttg
      661 caccgcatcg gcgatttcaa atttcttctc accgctgtcc cagcccagac gcacttttac
      721 gctgacaggc aaatgcgccg gtacagcttc gcgcatcgct tttgcaccct gatagatgag
      781 ttcaggatct ttgagtaacg tcgccccacc gccgctaccg ttaaccgttt tcgacgggca
      841 gccgcaattg agatccacgc cccaggaacc taactcgacc gcacgggcgg cattctctgc
      901 cagccattgt gggaactgac ctaacaactg cacgcgcacc agcgtaccgg ctggtgtccg
      961 gctggcgttt tgtagctcag ggcaaatgcg atgaaagact tttaccggca gcagttgatc
     1021 caccacgcgg acaaactcgg tgatgcacag atcgtagtcg ttaacttcgg tcagcaattc
     1081 acgcaccaga gagtcgagta caccctccat cggtgccagt aacacacgca tatcatcacc
     1141 cgcaaaaaaa tgaggcgcta tgttagcgcc tatggtcagc ggattaaagt actcagcgtg
     1201 gttgcaaaca gttatcttct ttccagccgt acagccactc catgccgcgg taaaactctg
     1261 cctgcgattt ccctttccac aacaggttac gtacctggcg ctctactcct gcggcatgat
     1321 aaacccgccc catttcacgg gtcgaccaga cgatgcgcgc ggtgcgcgga atacgtaccg
     1381 actcatacag cgcaaaggct tgtgccgcat caccctcaca ctgcgccagc gccttaccca
     1441 gtgttaccgc gtcttccagc gccatacagg caccctgcgc catatactgc gccaccggat
     1501 gggccgcatc gcccaccagc gtaatgcgct tcgttcccca cttcgctacc ggttcacggt
     1561 cggcggttga ccagcgtcgc caggaggtcg gtttatccag catctggcgc ggacgggggt
     1621 gaatcccttc aaagtaagag agcacttcct ctttactgcc atccttcacg ccccactctt
     1681 cctgctggcg gctgtggaat gtcaccacca ggttatactg cttgccgccg cgtaaagggt
     1741 aatgcaccaa gtgacagcac ggccccgccc acagtactgg tgcgttaatg cgcaaatctt
     1801 ccggcatatc ggcacaatcg ataacagcac ggtagaccac atgcccggtg acacgcggcg
     1861 catcgcccaa taagctctgg cgcaccactg acttcacgcc gtcacagcca atcagaatat
     1921 cgccgatcca actattgccg cgatcgtcga aaacggtgac atcgtcttct gtctcgcgaa
     1981 tatccacgac gttggtggag gtgtgatatt ccacgtccgg atgcgtcagt accgcttccc
     2041 acaccgaggc gtgaatatca acccggtgga tcaccgcgta agggccgccg aaatgatcgc
     2101 gaaaggcctg accggtttca atgcgcacca cttcttcggc gtttaccgca tccatcatgg
     2161 tgatgtgatc ggtgaatacc gcgcgctggc gggcgatttc gccgacgccg aggctatcca
     2221 acgccgaaaa cgcatttggc cccaactgga tacctgcgcc aatctcaccg atctcatgcg
     2281 ccttttccag cagcaccact ttgatcccct ggcgggccag tgacaatgca gttgccgcac
     2341 cgccgatccc gcctcccaca attactgcgc gtgtcacttt agtcattatg gttctcctgg
     2401 tcaggctgaa attttatctt gttgattttc tggcgctgcg gcaatgaacg ctggcagctg
     2461 cgtgcaggcg tcgtataccg ctttgcagcg cggatacccg ctcagatcgc agttcatacg
     2521 tagcgcgttc gcccactgtg gaacaaggca gcaatctgcc agcgtcggcg tctcgcccac
     2581 acaaaattgc cccgactggc tctggcgcag tagctgttcc accgcgctta acccttgctg
     2641 gatccagtgc gcataccagc gttttttatc ttcttcactg acgttcagct cttcggtcag
     2701 gtagcgcagc acgcgtaggt tattcaatgg atgaatatcg caggcgatgg catagacaat
     2761 ttccagcacc ttgttgcggg ccggttcttc ctgcggcatc agcggaactt gcgggtagtg
     2821 actctccagc cagtcaataa tcgccagcga ttgtccgagt gattcgccct cgtcggtcag
     2881 cagcgtcggc accagcccga ccggattcat ccgccggtag gccagttcgt tttgctgacc
     2941 aatgcggata ttcacccctt cggtctggta atcgataccc ttcagcgcca gcgcaatgcg
     3001 cacccggtaa gacgctgaac tattaaaaaa actgtacagc ttcatcatca cctcagacaa
     3061 tcttcaccgc gataggcgtt aagccctcga cgttgcccgt gatcacatca cctttgacta
     3121 ctgagccaac cccttccggc gtaccggtaa agattagatc gcccggttgc agttcaaaga
     3181 agcctgacag gtagctgatg gtttcattca ctgaccagat gagatgacga atatcgctgc
     3241 gctgatgatc ttcgccgtta acctgtaacc agataggtgc gttatcaata ccgccagttt
     3301 cgctggcttt gtgcagcggc gcgatggggg ccgagagatc gaacgctttg ccgatttccc
     3361 acgggcggcc catctggcgc atttccattt gacggtcacg gcgagtcata tcaagacccg
     3421 tggcataacc ccagacatat tcatgggcgt tttccagcgg gatatcactg ccttttttac
     3481 caatcgccac caccagttca atttcataat ggtagttatc ggtctgcgcc ggatacggca
     3541 gcgccagcgt ttcgccagcg gcaaccggaa caatggcatc cgccggtttg cagaagaaga
     3601 atggcggttc acgatccgga tcaaagccca tttcgcgggc gtgggcggca tagttacgtc
     3661 cgacgcagta aacgcggcgt acggggaatt gttcatcact gccaacaacc ggtacggtaa
     3721 cgggcgcttg tggttcgaat acgtattgag tcattttctc tctcccaaat taataacgtg
     3781 cttcgcggaa cagccccagg gcttcttgta ccggtctgtc cgaaaaacta aataaaacgg
     3841 tatcttgcgt ggtctggaac gacacgccgt gccaggtcgg caccacgaag atatcttttg
     3901 cactaaagct gaaggtttca ttgccgatag tgacctgccc gctaccttcc accacatggt
     3961 agatggtgct gtcagtggta cgcgccacgc gtgaggcaaa ccctttcggc agcagttgca
     4021 gaaaagcgcc catcgacggc atcggatagc caccggtgac cgggttgacg tagcgcattt
     4081 tgtagccatc ccactcatcg gcatcgccca ggcgggttaa atcgtgtagc acttcgcggc
     4141 tacggtcata acggtagtta aagatgggcg aggagttccc ggtctgatgg cgcaacggca
     4201 acatattggc agcgtaacgc ggcaggtaat cgccctcttt acgcgttact ggttgttgct
     4261 cctccgggta atcttcggca aaaccgcagc ccagagtgtt cactaatggc agatccagcc
     4321 catcgagcca gataaccggt tcgtcgccag ggttaccgtg atcgtgccag cgccactgcg
     4381 gggtcaggat aaaatcgccc tcattcattg acgtgcgttc gccatcaacg gcggtgaatg
     4441 cccctttacc ttcaacaata aaacgcagcg ccgactggtt atggcgatga ctcggtgcca
     4501 cttcgcccgg catgatcagt tgcaaaccgg catataaagt cgcggtgatg gaagactgcc
     4561 cgcgcagcgc cgggttttcc agcaccagca cgcggcgcac ggcttctttc gcgccaatca
     4621 ggctgccgct ctccagcagc aacgggcgga tctcctgata attccagtac gctggcgcac
     4681 agttagcgtt gggcgttttt ggcaccagat ggtgcagtga ctcccacagc ggggtcaggt
     4741 tttgcccgga aatatgctga taaaattgct cgcggctatt ttgattgtta tcagtcattg
     4801 ggatgctcct tattcgttca caacacctgc gtgaggcagt gagtcaggga cagaggtcga
     4861 ctggcgaacc acggtgatca gctgggttaa catcaccgca ctgatcgccg cgggcacagc
     4921 gataatgaaa aacagggtgt cgaaagagaa attcatcgcc atcataacgc cgccagacag
     4981 cgagccaaca atcgcgccgc agcggcccac ggcgtttgac cagctcacgc ccgtggcgcg
     5041 actttgtgtt ggataaagtg ttgcggtcag cgcattcagc cccacctgcg atccgctgat
     5101 gccaatgccg gtgccgaaaa tcgccagcgc cattagccat aatccgtcct ggcttaagcc
     5161 aatcatcacg atgcatacag cgcctatggc atagctcagc gccagtacgc ggaaaggatt
     5221 gaacttatcc ataagcacgc ccagcgccag cgcgcccaac gtaccgccaa tctggaacgc
     5281 ggcggtaacc caggaggcat gttgcaggtc gatgccgcga tggttgagca gcgttggcat
     5341 ccagcttgag agcaggtaaa taatcagcaa gctcataaag aacaccaccc acagcatcaa
     5401 cgtgatgggt aactgacgcc ccataaacag ttgacgaatg ctgccttttg taacggaggc
     5461 tgattcaatg agataaaagt gggtatcgac ataacgctcg cggatgatgg cgctgacggt
     5521 tttcgcaatc accgcctggg gtagctggcg gcgcacctgc cagcgtggcg attccggcag
     5581 aaccaccagc agtgcaacaa acagcatcag cggtaatacg ccacccaaca ccaggatccc
     5641 gtgccagcca atcaccggca ccagctgcgc gctgacaatc ccgccaaacg ccgaacccag
     5701 ggtaaagccg caaaacatca gcgtcaccag cgcgccgcgg cggcgggcag gcagatattc
     5761 cgaggtcatg gtaattgtat ttggcatcgc gccacccagc ccaaggccgg tgaggaaacg
     5821 caggaatacc agcgtttgca gatccggtga gaaagccgag gcgaggctta acgcgccaaa
     5881 caaaaagacg cacagctcaa tcacccgttt gcggccaaag cgatcggaca acggaccaca
     5941 aagcagtgcg cctgcggtta accccagcag acccgcgcca aaaagcgggg cgagatcgcc
     6001 tgcggtgagt tgccagtgag tgcgaatatc aggggcgata aagccgatag ccgcggtgtc
     6061 aaaaccatcg agcattacca ccagaaaaca gcagatgata acgcgccact gcatcttgcc
     6121 gaccggagca gcatcgatca gtgcttgtaa atcacgtcgt tgagtcatag taaatgcctc
     6181 agtgcaggta aggtagatgt ttcatttgtt ttatgtatgt tgcgatattg tgattaaaga
     6241 gatgaaatgt acaatggcat ttcggacaga gagataacct ggaggttatg gcaaatggca
     6301 aactgggcgc agaaactaaa gcttcatcac ctgcaaatgc tggtggcgct gggtgaacag
     6361 ggcaatctga cccatgtcgc gcggatgatg aatatcaccc agcctgcgct gtcgaaatgg
     6421 ttgtcacaac tcgaagatga gatcggcctc acactctttg agcggcacag caaaggactt
     6481 cgtcctactg aaggcggcaa attgttattg caacatgccc aacggttaat caatgacatg
     6541 gagcgctcgc aacatgaact ggcccgtttt aaacaaggtg gactggtcgg cagtctgaaa
     6601 attggctgtt ctcccgtcgc cactgactgc gtatcccagg cgattttgca tttgctcaag
     6661 gagatgccaa ctctacacct gaatatcgaa gagaaggtga tgaccccatt gctgcaagat
     6721 ctgttggcag gttctgtcga tgtggtggtt gggcgaattg gcggtcgcgc gttgcagctg
     6781 ccgctgaatt atcgcgtgct ctacaccgag ccggtgtgtt ttgtcgcccg tcctgagcat
     6841 ccactggcga aatacgccac cctcagctgg aatgatcttg ctaactggcg gtggatagtc
     6901 tggccgacag gtacccccat ccgcatcagc attgataatg cgctggtaga ccacggcgtg
     6961 atgctgccag aaaacacgat tgagtcggcg tcgatgaacg tcagtaccaa cctgttacaa
     7021 agcagcgata tgatctctat tttgtcatta cgcctggcgc aacgttatgc cagccaggga
     7081 caactggtga ttctcaacct gccaaagata gaacagaaag gcagcgtcgg tgtgttctgg
     7141 cgcaataacg aaacgccttc ttatgcttta agtcagtttc tgcacttttt acagcagaat
     7201 tagtgtgctg aactgggcgc tcgcccgagg ttacgtccct aaaattcaac tatgctgaga
     7261 tttcttaatg tgatcggtag cacgttttac gaattaattg tatgatgaat ccatctcatc
     7321 tggggtgttg attatgagta agacactgaa cattatctgg caatatttac gtgcttttgt
     7381 cctgatttat gcctgcctgt atgcaggcat tttcattgct tccctgctac cggtaaccat
     7441 tccgggcagc atcatcggga tgctgatcct gtttgtcctg ctggccttgc aaattcttcc
     7501 ggcgaaatgg gtcaatccgg ggtgctacgt actgattcgc tatatggcgc tattgtttgt
     7561 gccgattggt gtaggcgtca tgcaatattt tgatttgctc cgcgcacagt ttggtccggt
     7621 agtggtttcc tgtgcagtca gtacgctggt ggtttttctg gtggtgagct ggagttcaca
     7681 actggtgcac ggtgaacgta aagtcgtagg tcagaaagga tcagaagaat gatggcgaat
     7741 atctggtggt cattaccgct gacgttgatt gtcttttttg ccgcccgcaa actggcggca
     7801 cggtataagt ttcccctgct taacccgttg ctggtagcaa tggtggtgat cattcctttt
     7861 ttaatgctga ccggcatctc ttacgacagc tactttaaag gtagcgaagt gctaaacgat
     7921 ctgcttcaac cggcggttgt cgcactggcc tatcctttat atgagcagct gcaccagatc
     7981 cgcgcgcgct ggaaatcgat catcaccatc tgttttatcg gcagcgtggt tgcgatggtg
     8041 acggggactt ctgtggcatt gttgatgggc gcttcaccgg aaatcgccgc gtcaatcctg
     8101 ccgaaatcag tcaccacgcc tattgcaatg gcggttggcg gcagtattgg cggtattccg
     8161 gcaatcagcg cagtttgcgt gattttcgtc ggcatcctcg gcgctgtgtt tggccataca
     8221 ttgcttaatg cgatgcgtat tcgtaccaaa gctgcgcgcg gtctggcaat gggcacagcc
     8281 tcgcacgccc tcggtacggc gcgctgcgcc gagctggatt atcaggaagg tgcatttagt
     8341 tcgctggcgc tggtgttatg cgggattatt acttcgctga tcgcaccgtt ccttttcccg
     8401 attattctgg cagtaatggg ctaaaatttg cgatgcgtcg cgcatttttg atgtaagttt
     8461 cacgcgttgc ataattaatg agattcagat cacatataaa gccataacgg gttcgtaaac
     8521 tgttatccca ttacatgatt atgaggcaac gccatgcatc cacgttttca aaccgctttt
     8581 gcccaacttg cggataactt gcaatctgca ctggaaccta ttctggcaga caagtacttc
     8641 cccgctttgt tgaccgggga gcaagtctca tcgctgaaga gcgcaacggg gctggacgaa
     8701 gacgcgctgg cattcgcact acttccgctg gcggcagcct gtgcgcgtac gccattgtcg
     8761 aattttaatg ttggcgcaat tgcgcgcggt gtgagcggaa cctggtattt tggtgccaat
     8821 atggaattta ttggtgcgac aatgcagcaa accgttcatg ccgaacaaag cgcgatcagc
     8881 cacgcctggt tgagtggtga aaaagcgctt gtagccatca ccgttaacta tacgccttgt
     8941 ggtcactgcc gtcagtttat gaatgaactg aacagcggtc tggatctgcg tattcatctg
     9001 ccgggccgcg aggcacacgc gctgcgtgac tatctgccgg atgcctttgg gccgaaagat
     9061 ctggagatta aaacgctgct gatggacgag caagatcacg gctatgcgct gacgggtgat
     9121 gcgctttctc aggcagcgat tgcggcggca aaccgttcgc acatgcctta cagtaagtcg
     9181 ccaagcggtg tcgcgctgga atgtaaagac ggtcgtattt tcagcggcag ctacgctgaa
     9241 aacgccgcat tcaacccgac tctgccaccg ttgcaggggg cgttaattct gttgaatctc
     9301 aagggttatg attacccgga tatccagcgc gcggttctgg cagaaaaagc cgatgcgccg
     9361 ttgattcagt gggatgccac ctccgcaaca ctgaaagctc tcggctgtca caatatcgac
     9421 cgcgtgcttc tcgcttaagc ctggtgccgg atgcggcgtg aacgccttgt ccggcttgcc
     9481 agccctctcc tggtgtcgaa aatcccggca aacagtttgc cgtttcttgc gcaaaaccag
     9541 cgggtaaagt agcctgatgg aaattttcct tagatcgagt ctcctgcatg ttaaagcgcg
     9601 tgttcctcag cctgttagtc ctgatcggct tgctgctgtt gactgtgctc ggcctcgatc
     9661 gctggatgag ctggaaaacc gcgccttata tctacgacga attgcaggat ctcccctacc
     9721 gccaggtcgg tgtggtgctc ggaacagcaa aatattatcg tactggcgta attaatcagt
     9781 attatcgcta ccgcattcaa ggagcgatta atgcctataa cagcggtaag gtaaattatc
     9841 tattactgag cggcgataac gcattgcaaa gttataatga gccgatgacc atgcgcaaag
     9901 atttaatcgc tgctggtgtc gacccatcag atattgttct cgattacgca ggctttcgta
     9961 cgctggattc catcgtgcgt acacgcaaag ttttcgatac taatgatttc attattatca
    10021 cccaacgttt ccactgtgag cgggcattat ttattgcgct gcatatgggg attcaggctc
    10081 agtgttatgc cgtaccgtca ccgaaagata tgctgtcagt acgtattcgc gaatttgccg
    10141 cccgtttcgg tgcgctggct gacctttata tttttaaacg tgaaccgcgt tttttagggc
    10201 cgctggtccc tattccggct atgcaccagg tgcctgaaga tgcgcagggg tatcccgccg
    10261 tcacacccga acagttactt gaattacaaa agaaacaagg aaagtagtta tggatgtaca
    10321 gcagtttttt gtcgttgccg tttttttcct tatcccggta ttttgtttcc gcgaagcatg
    10381 gaaaggctgg cgcgcaggcg cgattgataa acgggttaaa aatgcaccgg aaccggtgta
    10441 tgtctggcga gcaaaaaatc ccggactctt cttcgcttat atggtggcat atatcggctt
    10501 cggaatttta tctatcggca tgattgttta tcttattttc tatcgttaat attttctctt
    10561 ttctgttaat tattgagaat aattattact tcactcaata agctgcggat ataacattct
    10621 gaaccgcagc tatttgtgaa tcctttcaca gtttaaattt ccccacgcgc atagccttaa
    10681 tatcagcacg caatatttat actctaaata attcgagttg caggaaggcg acaagtgagt
    10741 gagtccccag gagcttacat aagtaagtga ctggggtgag cgaacgcaga cgcagcacat
    10801 gcaacttgaa gtatgacgag tatatttagc gttcgcctta attactcata gcattaagga
    10861 agatcacatg ccgcaacaaa attatctgga tgaactcact ccggctttta caccgttact
    10921 ggcgattaaa gaagcctctc gctgtttatt atgccatgac gccccctgta gtcaggcttg
    10981 cccggcacag accgatccgg ggaaatttat tcgctcaatc tactttcgta attttaaagg
    11041 cgctgccgag acaattcgtg aaaataatgc cctcggtgcc gtttgtgcca gagtgtgccc
    11101 gacggagaaa ttatgccaaa gcggttgtac ccgtgccggt gttgatactc ccattgatat
    11161 cggtcgctta cagcgttttg ttactgattt tgaacaacaa actgggatgg aaatttatca
    11221 gcccggtact aaaacgctcg gcaaagtcgc cattattggc gcgggtcctg ccggattgca
    11281 ggccagtgtg acactgacaa accagggtta tgacgtcacg atctatgaga aagaagcaca
    11341 acccggtggc tggttgcgta acggcattcc gcaattccgg ttaccgcaat cagtactgga
    11401 tgcagaaatc gcccgtattg aaaaaatggg cgtgaccatt aaatgcaata acgaaatcgg
    11461 taacacactc accctggagc agctgaaagc ggaaaaccgc gcggtactgg tcactgtggg
    11521 gttatcgagc ggttcggggc taccactgtt tgagcatagc gatgttgaga ttgccgtcga
    11581 cttcttgcaa cgtgcacgac aggcgcaagg cgatatcagc attccacaaa gcgcattaat
    11641 tatcggcggc ggtgatgtcg cgatggacgt agccagcacg ctgaaagttc tcggctgtca
    11701 ggcagtaact tgcgtagcgc gtgaagagtt agatgagttt ccggcaagcg aaaaagagtt
    11761 tgccagcgcc cgggagctgg gcgtttcgat catcgatgga ttcacgccag tagctgtgga
    11821 aggcaataaa gtcacgttta agcatgtacg gttaccgggc gaactgacga tggcggcaga
    11881 taaaattatt ctcgccgtcg gtcagcatgc cagactggat gcctttgcgg agttagagcc
    11941 gcagcgtaac accatcaaaa cacaaaatta ccagacccgc gacccgcaag tctttgctgc
    12001 tggcgatatt gttgagggtg acaaaaccgt ggtttatgcc gtgaaaaccg ggaaagaagc
    12061 cgctgaggcg attcatcact atttaaaggg agcttgctca tgttaacgaa agatctttcg
    12121 attacctttt gtggtgtgaa gtttcccaac ccgttctgcc tctcttcttc gcctgtaggc
    12181 aactgctatg agatgtgtgc caaagcctac gacacaggct ggggtggcgt ggtgtttaaa
    12241 acgatcggtt tttttatcgc caacgaagtc tcgccgcgtt ttgatcatct ggtgaaagaa
    12301 gataccggtt ttatcggctt caaaaatatg gagcagattg ctgaacatcc gttggaagat
    12361 aatctggccg cactgcgtcg gctgaaagaa gattacccgg acaaagtatt gatcgcttca
    12421 atcatgggcg aaaatgagca gcaatgggaa gagctggcgc gtctggtgca agaagctggc
    12481 gcggatatga tcgagtgtaa cttctcctgt ccgcaaatga cttctcatgc gatgggtagc
    12541 gatgtcgggc aaagcccgga gctggtagaa aaatattgtc gggcagtaaa acgtggttcc
    12601 acgctgccaa tgctggcgaa gatgacgccg aatatcggtg atatgtgcga agtggcgctg
    12661 gcggcgaagc gcggcggcgc agatggcatt gcggcgatta acaccgttaa atccatcacc
    12721 aatatcgatc ttaatcagaa aatcggtatg ccgatcgtta acggtaaatc gagtatttcc
    12781 ggatattccg gtaaagcggt aaaaccgatc gccctgcgct tcattcagca aatgcgtacc
    12841 catccagaac tgcgcgattt cccgatcagc ggtatcggcg gcattgaaac ctgggaagat
    12901 gcggcggagt ttttattgct cggtgcagcg acgttgcagg tgaccaccgg cattatgcag
    12961 tacgggtatc ggatagtgga agatatggca agcgggttgt cgcattatct cgccgatcaa
    13021 gggtttgatt cgttgcagga gatggtaggt ctggcgaata acaatattgt cccggcggaa
    13081 gatttagacc gcagttatat tgtctatccc catatcaatc tcgataaatg tgttggctgt
    13141 ggacgctgtt atatttcctg ttacgacggc ggtcaccagg cgatggaatg gagcgagaaa
    13201 acccgtacac cgcattgtaa taccgagaaa tgtgttggtt gcctgctttg tggtcacgtc
    13261 tgcccggtgg gttgtattga tctcggagaa gtgaagttta agaagggaga gaaagaacac
    13321 ccggtaacgt tgtaaaacca gagacgcatc cggcatttgg caccgattgc cggatgcggc
    13381 gcatccggca atacccacat tcacatcatt tcttacgcgc gtatttcagt gaatccagcg
    13441 ccacggcgaa gataataatc gcccctttga tgatgtactg ccagtatggg tttacgccga
    13501 tgtacgtcag gccatagttg atgacggtaa aaataatcac cccggtcacc acgccaatca
    13561 ccgtccctac accaccgctg aacgatacac cacccaccac acatgccgcg atagcatcca
    13621 actcatacat aaagccgagg ttgttggtgg cagagccgat acgtccggct tctaacatcc
    13681 cgccgaaggc atagaacacg ccagacaacg cgtagatcat cagcaggttc aggccgacgt
    13741 tgacaccaga tactttcgcc gcttccgggt taccgccaat ggcaaaaatg ttcttaccga
    13801 agcgggtttt gttccacaac acccagacga acgccaccgc aatcaacgcg tagaaggtga
    13861 tgtaagagag acggaaactc cccagcgcga caaagccctg agcaaaggta gagaagccac
    13921 tgtcgaaacc agaaatcggc gacgccccga caaagtcgta atagagcgag ttgatgccat
    13981 agacgatgat catcgtgccg agcgtggtaa tgaacggcgt cacgttgaga taggcgataa
    14041 tcagaccgtt gatcaaaccg atcaccgcac caatggcaca gacaatcaga ataaccagcg
    14101 caatcggcat cgtcgccatt tccgggaaca ctttgttggc gttatccatg gactgcaata
    14161 acgtcgccgc tactactgcc gccagcccca cctggcgacc tgcagaaagg tcggtcccct
    14221 gggtgacaat taaccctgcc acaccgagcg cgataataat acgcaccgat gactgggtga
    14281 gaatattact taagttcaac agacttaaaa atgttgggtc ctggaaaata ataattgcca
    14341 gcaaaactaa aagaacgacg taaataccgc cctctttcag gtaagtaaga aaacttttct
    14401 tatttaacgc actcatggga agcccctaat cttaaaggtg caaagacgca agacgcagaa
    14461 tttcgttttg cgttgttgtt tttgtatcga caattccgga aacgagacca ttgctcatga
    14521 ccagaatacg gtctgttatc cctaacaact caggcatttc agaggagata ataataatcc
    14581 ctttgccttt cttcgccagt tcggcaatta actgatagat ctcaaacttc gctccgacat
    14641 caataccgcg cgtcggttca tcgagcatta atatttctgg ttgcgttagt agccagcgac
    14701 caataatcac cttttgctga ttaccgccgg agagcgaacc aatttgcgtc cgatgccccg
    14761 gtgtttttac ccgcatcgaa tcaatcaccc actgggtatc gcttttcatc cgcgagttat
    14821 ccagtaaacc aactttattt ttgtagttgc gaatattgga aattaacgag ttaaaaccga
    14881 tatccagata ggcataaatt cccgttgagc ggcgctcttc agttaccagt gcaaatccgt
    14941 ggtttatggc ttcgttggca ttatggttat tgatcttttt gccgtgcaac gtaatggtgc
    15001 cagccgattt ctcgcgaata ccaaataacg tctcaacaat atcggtacgt ttcgctccca
    15061 ccagaccggc tataccgaga atctcccctt tatgcagatc aaacgagaca tcgcgaatcg
    15121 acggctggcg cagcgacgtc aggttacgca cttcaagaat cacttcgccc ggcttgtttt
    15181 ctttgtcagg gaaacgctgg ttaagagaac gcccaaccat catggcgatg atcttgtcca
    15241 tcgtcagtcc tgccagcggt tcagtggcga tccactgacc gtcgcgcaat acggtaactt
    15301 catcacataa ctggaagatt tcttccattt tatgtgagat ataaacaata ccgcagccgc
    15361 gctcttttaa tttacgaata atagtgaaca gatgattgac ctctttttcg gttaacgaag
    15421 aagtcggttc atccataatc acaattttcg cgttatagga aaacgctttg gcgatttcga
    15481 tcatctgcat ttgcgaaacg gataatgtgc cgacgcgcgc acgcggatcg atatcaatat
    15541 ccagttcatc aaaaatcgct ttggtttcgc ggtacatttt gtcctgatcg acaaacatgc
    15601 ctttggtggg atatcgcccc agccacatgt tgtccatcac cgaacgttgt aataccaggt
    15661 ttaactcctg gtgtaccatc gaaataccat tttccagcgc ttctttggca gaatggaaat
    15721 cgatctcttt accctggaat aaaatggtgc cggagtcttt ttgataaata ccaaacaggc
    15781 attttaataa tgttgatttt cctgcgccgt tttcccccat taatgcatgg atagaatgtg
    15841 gccggacttt taaattaacg ttatcaagtg ccttaacacc tggaaaagac ttgttgatac
    15901 cgctcatttc caacaagtat tccccggaag acggagtcgt tgagctgacc atataatttt
    15961 accttgttgg ccatacaata agggcgcagt aaacgactgc gcccaatcag tcttatttct
    16021 tgctgaattc agccaggttg tctttatcta cgccaacata aggtacgcgg accactttgt
    16081 tgtcgatttt ccagttggtg ccatcagccg cacctttgcc atcggccagg tttttcgcca
    16141 gatcaaaggt cgctttcgcc tggttgttag catcgttcag tacggtgccc gccagtgcac
    16201 cggatttcac cagcgccagc gcttctggca gcgcatcgac gccaaacacc ggaatgctgg
    16261 acttgttgtg tgctttcagt gcttctaccg cgcccattgc catcgcatcg ttgttggcga
    16321 taaccacttc gattttgttg gcgttcgggc cagacagcca ggcgtccatc ttatctttcg
    16381 cctgagcggt atcccacata gcggtatcta actgtaactg ttcagttttg atgcctttgt
    16441 cgttcaattc tttaatcacg taagtggtac gtgcttctgc atccggatgg cccggctcac
    16501 ctttcagcag tacgaactga atctgaccgt ctttgttcag atcccaaccc tgattcgccg
    16561 cccagtgttt agcaatcaaa tcgccctgaa taatgccgga ctctttggag tcagtgccaa
    16621 cgtagtaggc tttgtcgtag ctatccagcg ccttacgaga cggttctttg ttgaagaaaa
    16681 ccaccggcac gttttgccca cgcgctttct caatcaccgt acccgcagct gccgggtcaa
    16741 ccaggttgat tgccagtgcc ttcacccctt tcgccagcaa tacgtcgatc tgatcgttct
    16801 gcttggactg gtcattctga gaatcattca tcagcagctg aacatctggc gcggctttcg
    16861 catcttgctc aatagccttg cgcaccacag acataaagtt atcgtcgtac ttatagattg
    16921 ttacaccaat gcgagtatca gcagcgtgtg cagcggcacc gaataacatg ctggccatca
    16981 cagcaaacag ggttaacacc ttcttattca tggtatctcc ggtttttctt atgcagggta
    17041 gtgcttgaga taaatgctcg gcggggcagt agagttaatg aagtgttact gaacgccgaa
    17101 gctcactttt taaaattcgt tcttccatgc ccggtaacgc tccagaaaac ggctttaatt
    17161 gttgttatga cgctattacc tcggtgaaag tgattattca ctgttacata cgggttacaa
    17221 cgttaaaacg gtgcaatcat agctatcaca ttgttaagat actgtgaaat cactcacaga
    17281 ttgaaagcgg ttacatcgcc agatttgttg agttagtgat cgccgccgca ttctggcgcg
    17341 ttgcgacaga atggcgacgc actaacgtcg gcataaaaca gtggctggca cgcggatcaa
    17401 tattgcctgc tgccccctgc aaggccagtt cggtggctaa tttagccatt gaagcaatgg
    17461 gataacgcac agtcgttaat tgcgggtcgg tgtaacgggc gatgggaata tcatcgaaac
    17521 cgatgatgga gagatgtaac ggaatcgcaa tgccattatc ttttaatgct gtcagtgcgc
    17581 cagcggccat attgtcgtta taagcaaata cagcggtaag ttgtagattg cgccccagca
    17641 gttcaaccat cgccgcctca ccgcccggca tgtccggcgt acctgtgcca atccagcttt
    17701 ccggcggaat aatatcctgc tctttcaacg cactcatcca gcctgcttta cgcatggcgt
    17761 catcttcaat gccgtgactg gaagaaagat aaccaatacg ttgatgaccg ttattcagca
    17821 acatgcgcgt cgccattcgg gcaccgctga gattatccag gcaaacgcaa cgatgggcgt
    17881 accccggcac aacgcggttg attaacacca taccaggaat gttatccata aattgtgcca
    17941 gttcatcatc actcaatgct tttgagtgaa caatcaacgc attacaccgc tggcgaatta
    18001 acacctcaat ggcgtgacgc tctttttccg cttcatgata gctattgcct attagcacgt
    18061 atttctgatg ctgctgggcg accaaatcca ccgcttttac cagcgcacca aaaaacgcat
    18121 cagaaacgtc catcaccacc acgccaatgg tatcgctaac ctgagttgcc agcgcctggg
    18181 cattggcgtt tggccgataa tccagctcac tcacggcttt cattactgct tcacgcgtgt
    18241 cggcactgac cagcgtgctg ttattgagca cccgggaaac cgttgccaca gagacgccag
    18301 cctgacgcgc tacatcacga atggtgatca tattcactac ctgttatgag attgcagtaa
    18361 atgactgctt gctggcggct attttgtcag cactgaatac aggacttcgt gaatcgagtc
    18421 acagcaatgg aaacggttac agccgttttg ttaacgaatg tgatccagat cgttatcttg
    18481 atgttttaga tcttgccggc ccagctgcac gcaaagttaa ctgacgccag agccattcca
    18541 ccggcccctg gcggaagtaa cgcagccaga taacagagaa gaggatattc gccagccata
    18601 ccggaataac aaacgccagc agctccaggc ggtcaaaatg cataaacaaa ccgaggtggt
    18661 aaaaaagcgt ggtacaaatc agcgtttgca atagatagtt ggtcagcgcc atccgtccga
    18721 cgcaggcgat cgcaagcacc agcttaaagc ggctcaattg cggccagaag ccataaaaca
    18781 gcgacgcata gccaatcgcc tgaaacggcg cactcagttc ccgcggcatc tgaagtaaaa
    18841 aggcacacca gcgatacgcc cagtccagct gccattgcag ggcgatggca ggaaggttaa
    18901 tggtcacccc aatcgccacc agcacaaaac cagtacgacg atagtgacgt aagctgaact
    18961 gccctttcag ccagccgctg cgcatcagcg ccgcaccaat gagcatcatc cctgccagtt
    19021 gccagccata ctgtgcgccc agtgccagta aactgttgcc aacaccatcg gcacgattac
    19081 tgatcgcttc aacaccaccg tgaagtttcc agtacttttc atacaaaata gccgatgcat
    19141 caggtgtcca ggcgcggctg gtctggctat cggaaatcaa ccccaataac agcaaaacgc
    19201 caagccccac cagataaagc atgacgccgg tattaaacag gctttttacc gatggcgcat
    19261 cgcgcaccag ccgccagcag attaagccca ccagcccgta agccagcaga atatcgccgt
    19321 cccagaacaa taaaccgtga ataaagccca gcaagactaa cagcgttaac cgcgactgga
    19381 tccagcgtct gccacggggc agcaacattt gcaggccagc accaaacagc agcgcaaaaa
    19441 gcgtgaggaa tttcacctgg ccgatgagat cgagaaatgc ccaggtccat gcatcctgcg
    19501 gcgtaatagc gccgtaccag gcgggattaa gataagccgc ctttggtagc ccaaaggcgc
    19561 tgatgtttaa tagcaggatc cccagaatgg cgacgccgcg aacaaaatcg agcgtgacgt
    19621 tgcgccccat ggttcctgcc ttttaatcag ttgtgatgac gcacagcgcg cagaaattcg
    19681 tggcgcgtat tctgactgga tttgaacaat ccaccaagag aggtcgttgt tgtggcactg
    19741 gttgcgtcgc ggatgccacg cgctttcacg cagtaatgca ccgcgtcgat agagacagcc
    19801 acgttattgg tgcccagcag cgtttgtagc gcaatgagaa tttgctgggt cagacgttcc
    19861 tgtacctgtg ggcgctgggc aaagaactgc acgatgcggt taatttttga cagaccgatc
    19921 accgaatctt tcgggatata agccaccgtc gctttgccat cgatggtaac aaaatggtgt
    19981 tcacaggtgc tggtcagggt gatatcgcgc acggtgacca tttcatcgac cttcattttg
    20041 ttttcaatga gggtgatttt cgggaaattg gcgtaatcca gaccggagaa aatttcatcg
    20101 acatacattt tagcgatgcg atgcggcgtt tccatcaaac tgtcatcagc caggtcgaga
    20161 ttcagcagct gcatgatttc ggtcatatga ccagcaataa ggcttttgcg cgtttcgtta
    20221 tccatttcat gcacgggcgg gcgcagcggt gtttccagtc ctcgcgcaac taacgcttca
    20281 tgaaccaggg ccgcttcttt actgagtgat ggcatttatg atttctcctg caggtgtgac
    20341 gcctccgccc tgcgtggggg caaagttatt aagctgattt acagcctgat tattgtgcgt
    20401 gaggcggcgc acataatcca gtattcacag cgataattat tgtaattgcc gctgcctttc
    20461 atcagcagat gttaaaacat cgttatgcaa atacggaagt gaaagttact cacagcacat
    20521 tgaataaacg gtatgatgaa gaaattgcaa acaacacaac aaggagccac gcatggaaat
    20581 gctcgaagag caccgctgtt ttgaaggctg gcagcaacgc tggcgacacg actccagtac
    20641 cttaaactgt ccgatgacgt tcagtatctt tctcccacca cctcgtgatc acaccccgcc
    20701 accagtgctg tactggcttt ccggattaac ctgcaatgac gagaacttca ccaccaaggc
    20761 gggtgcccag cgcgtggcgg cagaactggg gattgtactg gtgatgccag acaccagccc
    20821 gcgcggcgaa caggttgcca acgacgatgg ctacgattta ggccagggcg caggctttta
    20881 tcttaatgcc acgcaaccgc cgtgggcgac gcattaccgg atgtatgatt atctgcgcga
    20941 tgaattaccg gcgctggttc agtcgcaatt taatgtcagc gaccgctgcg ctattagcgg
    21001 tcattcaatg ggtggtcacg gtgcgctgat tatggcgctg aaaaacccgg gcaaatacac
    21061 cagcgtttcc gcctttgcgc cgattgtgaa tccatgcagc gtaccctggg gcattaaagc
    21121 gtttagcagc tatttaggtg aggacaaaaa tgcatggctg gaatgggaca gttgtgcgct
    21181 gatgtatgcc agtaacgcgc aggatgcgat cccgacgctt atcgatcagg gtgataatga
    21241 tcagtttctt gccgaccagc tgcaacccgc agtgctggca gaagccgccc gccagaaagc
    21301 gtggccgatg acgctgcgta ttcagccggg atatgatcac agttactact tcatcgcctc
    21361 ttttatagag gatcacctgc gcttccatgc gcagtattta ctgaagtgaa agtccgcccg
    21421 gttcgccggg catcttctca tcagaagcga taatccactg ccataaagta acgacgtccg
    21481 tcttcgttat agctgtagtc gtcacgactg agatccttgt cgccaaggtt cagcacgcct
    21541 gcgcgcagtt tgacgtcttt agtcacctgc caggccgcac cggtattcca gatggtataa
    21601 ccgcccggtg ttttagccgt cgcgctgtcg gcgcgtttct gcccggtata gtgtccggaa
    21661 acatagaatg accagtcttc cagcgccagc ggtttccagt ccagcgtacc gttagcggta
    21721 tggaacggca gatcggataa aggtttattt tcgccgttgc tgacatcacg accatcgttg
    21781 taggtgtagt tgagtgacag tttccattca tcgttgaacg gaattttcag ctcggtttcc
    21841 acgccctgaa tacgtgcttt gttaacgttg tagtagctaa ataccggcac accgttcgct
    21901 gtaaaaccaa caaagttttg gtagcctggt gcagcattga cgtcagacgt acggctgatg
    21961 ctgatacgat ctttcacatc gttacggaaa acggtaacgc tggattcaac cccttccagc
    22021 cagccttctt cgcccatgta gtaaagcccc agctcccaac tttcgctggt ttctggtttc
    22081 agatccgggc tacccacaat cttacatgcg ccacggcagg aattgctcgt ccagtcaggg
    22141 ctaagttgca acagagaagg tgctttaaat gccgtcgccc agcccccttt caccgttacg
    22201 gtgtcggtgg cgttataaac caggtaggca cgcggactcc agtgttcacc gtaggtttca
    22261 tgatcgtcca tacgtacgcc ggtcgtcagc gccagcggct cgaagatccg ccattcatct
    22321 tcaacaaata gcgcgtactg gctggcagat gttttggagc tggttccccc agtcaggttc
    22381 accgcatcgc taagtttgtc gtgacgccat tcaccgccaa ccgtgagaaa ctgattaatc
    22441 gccgtcagcg gcaacgtgta tttgccgtcg accgtattgc tttcggaagt tatcgggctg
    22501 ctgttgccag ggtttttgtt ctcgactttc tcaccgtagt atttcagttc gctggtgccg
    22561 taatcccaac gcccattatg gctgacggag tagttctggc gttccaggcg gtttttgtcc
    22621 agcgagtcgg aatcacgatc ctgacggtcg aaaccgtatc cggcagtaaa atcgtgattt
    22681 tgattcggtg tccaggcaaa ttcgacattg ccgtcgcggc tggagaatcc ttcaatacgc
    22741 ggcgtttctc cggtatcggt ggtcgttgag ttttgcgggt catccttttc acgttttgcc
    22801 aggctgccgt aagctttcat tcccagcacg ccgtcaatta atggtccgct ggtaaagaat
    22861 tgaccgttat aggtatcacc gcgatcgcga tgttcctgaa cggtggtatc gacggtaacg
    22921 gtgcccgacc atttctgacc gatttttttg gtgatgatat tcactacacc gccgagcgca
    22981 tcggaaccgt acagcgacga catcgggcca cggaccactt caatacgttc gatggaatcg
    23041 accgggatcc agttcagatc gaaatcattg tggcggaaga cggcattgcg ggagttaacg
    23101 cgtttaccgt cgacgagaat cagggtgtag ctgctgtcca gaccacgaat actaacgccc
    23161 ttacggttat ccccttcgtt cgtcagttgt acgccaggca cttctttgag gacatccttc
    23221 agattctgta ccggttttcg ctgcaggtct tcctaggtaa tgacgctgat actggcaggt
    23281 gcatctttaa ggttttgctc aacggaagat gcagtgacaa ccatcgtttc gccatcatca
    23341 tcgaccgcta acacaggcca tgcacaagaa atagcggaca aacacagccc gacccgtacg
    23401 aaagggttca acctaaacat tccatatctc catgaggtaa ctacgaaaat aaaatgggtt
    23461 atcgctcaca tcttcttcac gtccccttgc gtacggcagc atcgcggtgg acttatttgt
    23521 atgcaggtga tcatcccaga aagccagacc agcctctgat ttgtcagcct tttttgattg
    23581 cggcgtaacg ataatgcaaa cgataacaat tatcaatcca aatgttaaat tttatatccg
    23641 catgatttgt cagggaaatg gaattgaaaa agccctctcg gttgagaggg cttagcaagg
    23701 aagggagaaa acttatttct tatcgttctg cgggaacttc atttcgctgt agcgtacaaa
    23761 gtgagttcct ttaatcagct tgtagccgaa ccaaataatc aggaacagcg ggataccaat
    23821 atacgttgcc gctacgccgc cccagtcaat ggtatctttc aggaacgctt catagttctg
    23881 gcccaaagtg ataatcagac acagaatgaa tgcgaagatc ggccccagtg ggaagaaacc
    23941 tgaacggtac ggcagatcgt taatgtcgtg tccctgcaat acgtaaccgc gacggaagcg
    24001 atagtggcta acggcaatcc ccagccaggc gataaaaccg gtcatcccgg aggtgttcag
    24061 cagccacagg tataccgtct ggttgccaaa catggaggtc aggaagcaca gaccggcaat
    24121 caccgtcgtc gcatacagcg cattacgcgg cacgccacca cgcgacagtt tagcgaaaat
    24181 gcgcggcgct ttaccgtcac acgccagggt gtacagcata cgagtagacg catacatacc
    24241 ggagttaccc gctgacagca ccgccgtcag aataactgcg ttcatcaccg ctgcagcaga
    24301 gagcagaccc gcgtgctgga acaccagggt gaacggactg acgctgatgt ctttaacatc
    24361 gttacgcagc aggctcggat cggtgtacgg aataatcagg ctgataatca ggatcgcgaa
    24421 cacatagaac aacaggattc gccagaacac ctgacgtacc gcgcgtggaa tgtttttcgc
    24481 cggatcttcg gactcgcctg cagcaatacc gatcagctcg gttccctgga aagagaagcc
    24541 gacaatcata gctacgccga tcatcgccgc aaaaccacca gcaaacggcg cttcgccgat
    24601 tgtccagttg ctccagcccg caggctgcgc gcctttgaag ataccgataa tcatcagcac
    24661 gccaacgatg ataaagacaa taactgtcgt gactttgatc agtgagaacc agtattccgc
    24721 ttcaccaaag ccacgaactg agatgtagtt cagcaggaag ataacgccga ggaacagcgc
    24781 actccagatc cagcccggtg tatccgggaa ccaccagctc atgaccagct gagctgcaac
    24841 caggtcaacg gcgatagtca ccgcccagtt gtaccagtag ttccagccca gcgcgaagcc
    24901 aaagccttct tcaacatagt tctgaccgta agtggcaaac gaaccggaaa ccggcatata
    24961 tgcagccagt tcaccgagac tggtcatcag gaagtaaacc atcaggccaa tcaacatata
    25021 cgagagcaat gccccgcccg gacctgcctg agaaatcgtt gcgccagagg caacaaaaag
    25081 acctgtaccg atggaaccgc caatggcaat catcgtcagg tgacgcgcct ttaattcacg
    25141 gcgtaagccc ggcgcttctg tggttttagt ttcggaaacc atacaaaaat gctatccatc
    25201 ttaaaaatga ggcgcgattg tagcagacga ttggcaatcc ttccggcaga aatacacggt
    25261 tataagacac cttcatgatc gcccagggat tataagtaaa gcagccaatc tcttttctgg
    25321 cgaatgcatg ctgagggcgc tgcgtcacga aatcgacaca gcaccagcat gttcttgtac
    25381 agcaacttaa cgcggtacat tcgcgggatc gcaatagtcc agaaagcgcc gtagcgcgtt
    25441 ggaaaggtgt ttttgccgat gatgtatacg ccacaacgta cgcatcaggc gcggcagagg
    25501 gaccgcaact tcacttaatg tgcctgcctg caattgatct tcaatcaccc gtcgcgacag
    25561 gcaactaatt cccaacccat gacgcaccgc atgtttgatt gcctcggagt tacctaattc
    25621 catcgccatc tcaaacttcg gtaaatgtga cagcaacaga taatcgacaa tctcccgcgt
    25681 gccggagccg cgttcacgca ggatccacgg cgcagcggcc agctgttcta aggtgaccgg
    25741 accacgagcc aatggcgaag tcggcgcggc gaaaaccacc agctcgtctt ccagccacgg
    25801 ttcagaaatg atttcagtgc tgtggcacgg tccttcaata aagccaatat caacgcggaa
    25861 atccagcacc gcttgcatca cgtcctggct gttcccaacg ctaagttcaa tcggcaactg
    25921 cggataatca tggcgataac gggcaatgac tgcaggcaga atgtagttac cgatagtact
    25981 actggcatag atacgaatcg cgccgttgtc ttcgcgaaac agttgttcga tttcaaccgc
    26041 ctgttcaagc agtgccaacg cgcgcggata gagcagccgc ccgtgttcat taacaaccag
    26101 tcttttcccc acgcgatcaa acagttgcac gccaagctgc ccttccagat cggtcaaggc
    26161 tgcgctcact gctgattgcg acaacgccag catcaccgac gcctgggtgg ttgatccact
    26221 tttcaatact tctgcaaaaa cttccaactg ccggagggtg atgtgcatag tcgcttacca
    26281 cttataaaga ttaattataa atatataatc aattttattt ttaaaccagt tagtcgtaac
    26341 cttatgcccg gttaaaggag agggttatga cgaatatcac cttacagaaa caacatcgta
    26401 cactgtggca ttttattccg gggttagccc tgagtgcagt tatcaccggg gtcgccctgt
    26461 ggggtggttc cattcccgcg gttgcgggtg ccgggtttag tgccctcacc ctcgcaatct
    26521 tgttggggat ggttttaggc aacaccatct atccgcacat ctggaaaagc tgtgacggtg
    26581 gtgtgctgtt tgccaagcaa tatttattac gtctgggtat cattctttat ggcttccgtc
    26641 tgacgttctc gcaaattgcc gatgtcggta tcagtgggat catcattgac gtgctgacgc
    26701 tgtccagtac cttcctgctg gcttgcttcc tggggcagaa agtgtttggt ctggataagc
    26761 acaccagctg gttgatcggt gccggtagca gtatctgtgg tgctgccgcg gtactggcga
    26821 ctgagccggt agtgaaagcg gaagccagta aagtaaccgt ggctgttgca accgttgtta
    26881 tcttcgggac cgtcgcgatt ttcctctacc cggcgatata tccgctgatg tctcaatggt
    26941 ttagtccgga aactttcggt atctacattg gttctactgt gcacgaagtg gcgcaggtgg
    27001 tggcggcagg tcatgccatc agcccggatg cagaaaacgc agcagttatt tccaaaatgc
    27061 tgcgcgtgat gatgctggct ccgttcctca tcctgctggc ggcgcgtgtt aaacagctgt
    27121 ctggggcgaa cagcggcgag aaaagcaaaa tcactattcc gtggtttgcc atcttgttca
    27181 tcgtagttgc catctttaac tcgttccacc tgttaccgca gagcgtggtg aacatgctgg
    27241 taacgctgga taccttcctg ctggcaatgg cgatggcggc gctgggtctg accactcacg
    27301 tcagcgcgct gaaaaaagcc ggggcgaaac ctctgctgat ggcactggta ctgtttgcct
    27361 ggctgattgt tggtggtggt gcgattaact atgtgattca aagcgtcatc gcataaacca
    27421 ctacatcttg ctcctgttaa cccgctatca ttaccgtttt cctccagcgg gtttaacagg
    27481 agtcctcgca tgaaatacat tggagcgcac gttagtgctg ctggcggtct ggcaaatgcc
    27541 gcaattcgcg ccgccgaaat cgacgcaacc gcgtttgcct tgttcaccaa aaaccaacgt
    27601 cagtggcgtg ccgcaccgct cacgacgcaa accatcgatg aattcaaagc cgcctgtgaa
    27661 aaatatcact acacatcggc gcaaattctt ccccacgaca gttatctgat taacctcgga
    27721 catccggtcg ctgaagctct ggaaaaatcg cgcgatgcgt ttatagatga aatgcagcgt
    27781 tgcgaacagc tggggctttc tttgctcaac ttccaccctg gcagccatct gatgcagatt
    27841 tcagaagagg attgccttgc gcgtattgcc gaatccatca acattgcgct ggataaaact
    27901 caaggtgtga cagcggtgat tgaaaacacc gccggtcagg gcagtaactt agggtttaaa
    27961 ttcgaacatc tcgcggcgat tatcgacggc gtggaagata aatcccgcgt cggcgtctgc
    28021 attgatacct gccatgcttt tgctgccggg tatgatttgc gtacttctgc cgaatgcgag
    28081 aaaacgttcg cggattttgc ccgtattgtt ggctttaagt atctgcgcgg gatgcacctt
    28141 aacgatgcga aaagcacctt tggcagccgc gttgaccgcc atcatagcct cggtgaaggc
    28201 aatattggtc atgatgcgtt ccgctggatc atgcaggacg accgtttcga cggcattccg
    28261 ctgatccttg aaaccatcaa cccggatatc tgggcagaag agatcgcctg gctgaaagcg
    28321 caacaaactg aaaaagcggt agcctgaaga tgaacaaccg ggagaaggag atccttgcaa
    28381 ttttacggcg taacccgctg attcagcaga acgaaattgc ggatatgctg caaatcagcc
    28441 gttcgcgcgt tgcggcgcat attatggatt taatgcgcaa aggccggatt aaaggcaaag
    28501 gttacattct caccgagcag gaatactgcg tagtggtggg gacaatcaat atggatattc
    28561 gcgggatggc ggatatccgt tacccgcaag cggcttctca tcccggtacc attcattgct
    28621 cggcaggcgg cgttggacgc aatatcgccc acaacctggc gctgttaggc cgcgacgtcc
    28681 atttgctttc agtgattggt gatgactttt atggcgaaat gctcctggaa gaaacgcgcc
    28741 gcgccggcgt gaatgtctcc ggctgcgttc gtttacatgg ccaaagcaca tcgacgtatc
    28801 tggcaattgc caatcgagac gatcaaaccg tgctggcgat taacgatacc catctgctgg
    28861 atcagttgac accgcaacta ctgaacgggt cgcgcgattt acttcgtcat gcgggcgtgg
    28921 tacttgctga ttgtaacctg acagccgagg cgctggaatg ggtctttacc ctcgctgatg
    28981 aaatcccggt gtttgtcgat accgtttcag aattcaaagc gggcaaaatc aaacactggc
    29041 tggcgcatat tcacaccctg aaacccactt taccggagct ggaaatttta tggggacagg
    29101 cgatcaccag cgatgctgac cgtaatgccg cagtgaatgc gttgcatcag caaggcgttc
    29161 agcaactgtt tgtttatttg cccgatgagt cagtttattg cagcgaaaag gatggagagc
    29221 aatttttgct gacagcgcca gcgcatacga cagtagacag ttttggtgct gacgatggtt
    29281 ttatggcggg cctggtatat agctttctgg agggaaacaa tttccgtgac agcgcccgtt
    29341 ttgcgatggc ctgcgcggca atttcacgcg ccagcggcag cttaaacaac cctaccctgt
    29401 ctgccgataa cgcgctttca ttagtgccga tggtgtaaca atgttgccgg attcgacgct
    29461 tgactacatc ttatccggct cataactcgc gcactacgct aagccaataa agaatcctgc
    29521 aatggttgca ctcatcaggt tagaaagtgt cgccgccgcc agcgcgcgta aaccaagctg
    29581 ggcgatttcc ggtgcacggt gtggcgcaac cgcagaaaac gcccccacca ccaccccgat
    29641 agaaccaaag ttagcgaaac cgcacaacgc gaaggaaata atcgccacgg ttttagcatc
    29701 cagagtgcca gctgtttgca gataaggtga gaaattgagg taagcgacaa attcatttat
    29761 tgccagtttc tgtccaatca aactcccggc aagattcgca tcactccagt caacacccat
    29821 tacccacgcc agtggggcca gcaggtaacc taaaatggac tccagcgagg catgttcaaa
    29881 gccaaaccag ccgccaacgc cgccgataat accgttaatt aacgcaatga tggcgacaaa
    29941 tgccatcact actgtcgcca cacctgcggc gattttcagc ccggtcattg cccctgtcgc
    30001 agcggcttca ataatgcttt ttggcggtgt ttcggtgaaa gagaggttat taaaggaaac
    30061 ctgcgaagat tctgttgccg ggcttaacag gcgggcaaac aagatcccgc cggggatcgc
    30121 cattaacgat gccgccagca gatattccac aggcacgccc agtgcggcgt aaccaatcat
    30181 tgtcgaacca gcaatcgagg ccatcccgct acaaatcgct gtaaataatt cattgcgatt
    30241 cagacgattg ataaaggatt tgacgattgc cggaatttcg ttttgcccga ggaaaatggt
    30301 agtgaccgcg acgaatgact cgatcttgct gatattcaat gctttctgga agataccgcc
    30361 gagaatgcga attaagatcc ccatcacacc gatgtagtag agaatactca ccagtgctgt
    30421 aacgaagata attgccggta ataccctgaa accaaagata aatcctgcgc catcaaataa
    30481 ggtatccatt tttggtccga ccagagaacc aaagataaat gcgctacctg catcgctgta
    30541 cgccatcact ttatgcacgc caaaagcgac tttttctgcg acccaacgcc ctggtggtaa
    30601 ccaaagcata atgccgccaa tcacgacctg taacactaac gccgcgccaa cggtacgcag
    30661 gctgatcttc ttcttgttta ctgacagcaa aaacgcaatt gccagcaata ccaccattcc
    30721 cagaacactt ctcatgacat ccatagttat tatctcttca tgtcattttg ggcgacggct
    30781 ttctgcccat cagaaatagc cgggcctgca tcttgcgata ctgtcccggc ttgttatcaa
    30841 cacttaatgg gttttgatgt agccgcgcac gcactcttcg actaatcccc agaaccagtc
    30901 agtatcaata gtgatgccga ctttggtatt ggcgggcttg ccaagaacgc ccagctcgtc
    30961 gcagaccgta cgcccgtagc aagggccgct gttaacgtcc acttcgacgt acatctcctg
    31021 ggttttaatg ccatcagggt taatcagata accgatgcag gtggcgtcat gcaccgggcc
    31081 gccagccagg ccgtagtttt cgaactgggt tttgagagtg aagttcatga tgtcgctgaa
    31141 cagctctccg gcagggccgc ctgccctttc catccgagca atcacgtccg gggtgcaaac
    31201 ggtctggttg gtgagatcga ggcccatcat cactaatgga acgccggagg tgaacaccac
    31261 gcgtgcggct tccgggtcag caaagatgtt gaactcggca gatggggtga agttgccagt
    31321 accgtaagcg ccgcccatca gcacaatttc acggattttc ggcaggatcg cgggttgcat
    31381 acgcattgcc accgcgatat ttgaaagtgg gccaaccggc accaaagtga tatcgccatc
    31441 gcttgccatc agggtatcaa tgatatattt caccgcatga gtgctttctg cctggcgggt
    31501 cagcggctcg aataccgggc catccagtcc ggtttcaccg tgaatattat cggcaacgat
    31561 ttgttgacgc ataatgggct gtggcatccc cgcataaacc ggaacattaa tctccagttt
    31621 ctggcaaaca ttcaggccat taattaatgt tttatcaagc gtctgattac ccgccacaat
    31681 ggtgatacct aataaatcaa tcgccggatg tttcgctgcc atcattatgg caatagcatc
    31741 atcatgaccc ggatcacaat ccagaataat ttttctcttt tccattgttt atttcctctg
    31801 tttccagttg cgttattttt tctacagcaa agaaaattcg accgggccga tgattgaatc
    31861 ttaacaacag cgtaagtatg ctaaatatga gaaatctcat agcggataaa catcgtgaaa
    31921 gaaatccaca ataatgacct taagcagcaa ttgatgagtg aatctgcgtt taaggattgc
    31981 ttttcaacgg atgtttcagc cgatacgcgg ctgtttcatt ttttagcgcg tgactacatt
    32041 gtgcaggaag ggcaacagcc gtcatggctg ttttatctga cgcgaggccg tgccaggctt
    32101 tacgccacgc ttgctaatgg tcgcgtgtcg ctgatcgatt tctttgccgc tccctgtttt
    32161 attggcgaga ttgagttaat cgacaaagac catgaaccgc gtgcggtgca ggctattgaa
    32221 gagtgttggt gccttgcgct ccctatgaaa cattaccgcc cgctgttatt aaacgacacg
    32281 ctatttttac gaaaactctg cgtcacctta agtcataaaa attatcgtaa tattgtttct
    32341 ttaactcaga atcaatcatt tccgttagtg aatcgcctgg cagcttttat attactctcg
    32401 caggaaggtg atctttatca cgaaaagcat acgcaagcgg cagagtattt aggtgtttct
    32461 tatcgacatc ttttatatgt tctcgcgcaa ttcattcacg acggtttatt aattaaaagc
    32521 aagaaagggt atctcattaa aaacagaaag cagttgtcag gactggcact ggagatggac
    32581 ccggaaaata aattctcagg gatgatgcag taaaaattat ttgcaataga gcgattgccg
    32641 gatgtgacgc ttaataagcg cgtacatccg gcaatgacac tcaagctaaa ccaataaaga
    32701 acccggcaat ggtcgcgctc atcaggttgg aaagcgttgc tgctgccagc gcccgtaaac
    32761 caagctgggc gatttccggt gcgcgttttg gcgaaatagc cgaaaatgcc ccaacgacaa
    32821 caccgataga accaaagtta gcaaaaccac aaagcgcaaa ggagataatc gcaatggttt
    32881 tcacttccag cgtgccgccc gtttgcaggt atggggagaa actcaggtaa gcgacgaatt
    32941 cgttaatcgc cagtttctgc ccaatcaggc tacccgcaag attggcatca ctccagtcca
    33001 cacccatgat ccacgccagc ggtgccagca catagccaaa aatactttcc agagaggcat
    33061 tggcgaaacc aaaccagccg ccaattccgc cgataatgcc gttgatcagc gcaataattg
    33121 cgacaaacgc cattacaacc gtcgccacac cagcggcgat tttcagcccg gtcatcgcac
    33181 cgctcgccgc cgcttcgata aagctttttg gcggcgtttc gctgaacgac agattttcaa
    33241 atgtcacctg cgaaggctcg gtggctgggc taagaatacg tgcaaacaaa ataccaccag
    33301 ggatcgccat cagcgatgcc gctaacaggt agtcaattgg tacgcccatt ccggcataac
    33361 caatcatcat cgaaccagca atggacgcca tcccgctaca aattgcggta aacaactcgt
    33421 tgcgattcat gcgatcgata aacggtttaa cgatcgccgg gatctcattt tgcccgagga
    33481 aaatagtggt aaccgcaaca aaagattcga ttttgctgat gttgagggct ttctggaaaa
    33541 tgctgccaag gatgcgaatc agcagcccca tcacgccaat gtagtacagc agactgatga
    33601 gcgcagtgac gaagataatc gccggaagta cgcgaaaggc gaagataaaa cccgcaccgt
    33661 caaagaggac atccattttc ggcccgacca gcgaaccaaa aatgaaggcg ctaccggcat
    33721 cactgtaaga catcacttta tgaacgccta atgccgcctg ttctactgcc cattttcccg
    33781 gtgggaagta gagcatgatg ccaccaatag cgatttgcag cagcagtgcg gctccaaccg
    33841 tgcgcaaact gatgctcttt ttattcactg acaacagaaa tgctattgcc agtaacacca
    33901 ccatccccac aacacttctc attatatcca taatgatttt cccttcatgc cagtaaaccc
    33961 ggcgtcagcg ccaggttttg gtatgcttga tgagtacggg cgacggcttt ctgcccgtca
    34021 gaaattaacc cgcgagacgc tggtattctt tggctatttc actcgccagg atggcgttgt
    34081 tgaacaccag ctggatgttg gatttcaggc tgtcaccgcc ggtcaactca gcaacgcgcg
    34141 ccagcaggaa tggcgtactt tctttaccaa taacaccctg cgcttcagct tcagctaccg
    34201 cctgatcgat cgccgcattg atagtgtgtt ccggcatagc aaactgttcc gggatcgggt
    34261 tcgctaccac gaggccaccg ttcagcccgc tttgccattt caccgccatt gcacgggcaa
    34321 tttcgctggc gctgtcgaga cgaatgctca cgtcaaacgg gctggtgcgg cagaaaaacg
    34381 caggcagcgc tttggtctga tagccaatta gcggtacacc gaaagtttct aaatattcgg
    34441 tagttaagcc taaatcgaga atagatttcg ccccggcaca aacgacggtg acattggtgt
    34501 gcgccagttc ttgcaaatcg gcagaaatat cgaaggtatg ttccgccccg cgatgcacac
    34561 caccaattcc cccggtggca aatactttaa ttccggcaag cgccgcaata atcatcgttg
    34621 aagcaacggt ggttgcacca ttttttccgg cggcaacaac aaaaggtaaa tcgcgacgac
    34681 taactttggt cacgttatgc ccttcacgac ccagcaattc aatttcttct ttgcttaaac
    34741 ccactttcat cacgccgcca ataatggcga tagtggcagg tacagcgccc tgtttacgaa
    34801 tagtttcttc aacttcaatt gcggtctggg cattttgtgg gaacggcatc ccgtgagaaa
    34861 taatggtcga ttccagcgcc acaaccggtt ttttgttttt taaagcgtcc tgcacttccg
    34921 gggaaatttg taataattca ggggaaattt ttaattcaga cattctgcgt tctccactaa
    34981 cgatataacg ttggcaatcg ataaatcggg gttattggtg tattcacagg agagcgccat
    35041 tgacgaacat ccctgtgcga aacgaacaga ttcggcaaac ggcattccgt ctacccaaca
    35101 cgaagcaagt cccgccatca tggcatcgcc cgctccggta acattaatga cattggtttt
    35161 gatcggcgca gaccaaccac tttcaccgct gatatcactg taataaacgc cgtcgccgcc
    35221 catgctcaat accagtcggt tcaggccatg ttgatggaac caggcagcaa cttttgccac
    35281 atcttcacgc cctgacaaag caatcccact cagggtttcc gcttcaaggc ggtttggctt
    35341 gagagtgtgg atttgattta gacggtcgcg cactttgaca catttccacg cggaaacagg
    35401 atcgacaaat acgggtacgt tggcggcatt atccagaatc catgccagtg cctcttcact
    35461 gatattacag tccgcgacaa tgacctttgc cctctgaata aattcaccgt gctgtgcgag
    35521 atattcagct gtaatagcgt tgctaatatt catgtcattt atagcaacca gcatttcacc
    35581 ggtattatcg agtaatgata aatagctcga cgtattttct cccggcacaa tcaggcattt
    35641 atcgacataa acaccagatt gattggtttg cgttagcagc gattgaccat aaaaatcact
    35701 gcctacggcg ctcagtagcc aggctttatt ccccagcaac gccaggtttt gtgcaatatt
    35761 gcgccctact ccaccaggcg taaactttat tttacctgga tttgaatccg cataatttaa
    35821 tgattcatgt gaatatccgg cgacatcaat attcgccgaa cctataatta cgacataatc
    35881 cttttcgcgc atagcacgtc ctctggtaaa aaattaccaa tcctgtcaat ccacaagaat
    35941 atagatactg caaagacggt ttaaacatgt gttcatattt aaacatgtgc tcatagtatg
    36001 catgttttac gtaaagtaaa ttgccgccag agaggcaaaa tgggcagatt tgagcaggct
    36061 cacaaaatgc gattaaatgc ctgaattaca aaagtgagga atttgtgacg ggatgcacag
    36121 aacaggaaca ctaaaaaagg gcagaaaata atctgccctt gaggaaataa ccttagtgtt
    36181 tgatgaacat ttcaggtttc tactgaaaga taaaaccatt taaattcata aaattacatt
    36241 cacaaactgg tcattccctt tgcgctcatc aaatgttaca gggcaggaaa tttctgccct
    36301 gtaacacacc ttttattacg ctgctttcgc tactgcgtcc acttccggac gtttcaggaa
    36361 ggcataggcc aaacccgcca ccagcgtacc ggcaataatt gctaccaggt aacccagtac
    36421 cggcgtaata gcgccaggga tcagcagaac aaacagacca ccgtgcggtg ccataagttt
    36481 cgcaccaatc gccattgaga ttgcgccagt cagcgcccca ccaacgatac aacacggcag
    36541 cacacgcatc ggatcacgag cagcaaacgg aattgcacct tccgaaataa agcacagacc
    36601 aagaaccaga gcggctttgc caccttcctg ctgcgctttg tcgaatttgc gacgcgccac
    36661 cattgttgcc agacccattg ccagcggtgg caccatacct gccgccataa tcgccgccat
    36721 cgggccatag gtttgagtac tcagcagacc cacaccgaat gcgtacgctg ctttatttac
    36781 cggaccgccc atgtcagtac acatcatgcc accgaggatc gcccccagca gaaccgcatt
    36841 cgcagtcccc atggtctgca gccagtgagt caacccttcg agaatgccag caaccggttt
    36901 accgatcagg tagatcatcg ccagaccgac caccagactg gaaattagcg gaatgatcag
    36961 gatcggtttc agcgcctcca tactctgtgg cagtttcagt tgcgtactga ttaacttcgc
    37021 aatgtaacca gccaggaagc ccgcaataat acccccaatg aagccagaac cggtgctgac
    37081 cgccagcata cccccaatca gacccggcgt gagacccgga cgatcggcaa tggagaagga
    37141 aatgtaacct gccagtaccg gcaccatcag cgcaaaggct gaaccaccac caatctgcat
    37201 cagcgccgct gccaacgtac ccggctcttt aaacgcttcg ataccaaaag caaaagaaag
    37261 cgcgatacac agaccacctg caacgaccat cggcagcatg taagagacgc ccgtcagcaa
    37321 gtgacggtaa gcgcctgcac cctctttctt accttcagtg gtcgctgttt gagctttgcc
    37381 cgccggttca tacggcgttg cttcagcaac cgctttatcc agttcctgcg cggttttctt
    37441 cagcgccaga ccggtagagg tgcgatacat cggtttacca gcaaatttcg ccagatccac
    37501 ttcgatatct gccgccacaa tcaccagatc cgctgcggct acttcttcag gggtgatcgc
    37561 attacccgcg ccaacagaac cacgggtttc aactttcacc caccagccac gttttttcgc
    37621 ttcggtttca atggcttcag ccgccataaa tgtgtgtgct acgccagtcg ggcaagctgt
    37681 caccgcaact acgcgtttcg gaccgctggt ggcaactggt gctgtcgcag cgaccggtgc
    37741 agtgtaaggt ttcgcatggc ctttggcttc actcaggaac agctcagggt gcgcaactgc
    37801 ccgggaaata tcgcccagcc agacattttt accgttcagc gcgctgtcgt tcgggatgga
    37861 atcaccgaga acaatcgcca tttcagcgtc gttcggattg tcgatgattt ccagttttgc
    37921 ttttcgcgcc gccgcgccca gcagggtctt cgccatatag gcgcgtgcct gaccgagatt
    37981 agcgtcaata atcagcagcg ttttcattat gcctctcctg ctgtcagtta aaaggttgta
    38041 agtcgacgcg cgccatcatt gcggccaact gcggacgatc ggtaataccc acattgcttt
    38101 gacttaccgc cagggctgca acagctgtcg ccagacgcag tgtgtgttca ctggattcac
    38161 gcatcagcaa gccataaatc aggccaccaa ccatagaatc ccctgcgcca acggtgctta
    38221 cgacatcgac tgacggtggt ttggcgatcc attcgccgga ggcattaacc caaagcgcgc
    38281 cttcggcacc cagtgaaata acgacatgcg cgataccttg ttcacgcagc gcatgcgcag
    38341 cttcaatcac atctttcatt tcaggcagtt tacggcctgc ccagatttcc agctcgcggc
    38401 ggttaggttt caccagccac ggtgccgctt tcaaacctgc tactaacgct tcacggctac
    38461 tatcaaagat aatgcaagga cactgactac gcaggcgagt catccagtcg gtgaacgctt
    38521 ccgggctgac gcctgacggt aagcttccgc tgacacagac catatcgaac tgaccgagcc
    38581 agctcagaga atcagtcaca aagcgttccc agtcggcggg ggtaacgtca aaacccgaga
    38641 agttgaagtc ggtcacttcg ccgtcttttt ccgtcagctt aacgttaatt cgggtgcgcc
    38701 cctgtacaac ctggaaacgg ttggcaatgc ccagctcgct gaacagttgc tgaaaaccat
    38761 cctgattgtc tttaccgagg aagccaccaa cggtgacatc aattcccagg tcttttaata
    38821 ctttggccac gttgatgcct ttacccgccg catgcagacc ggtggttttc accaggttca
    38881 cttcgccgcg ttcaatttcc gggcagaaac caacaaggtc ataagccgga ttaagggtga
    38941 tagtagcaac acgtctgctc attatgcgcc ctccccaaga ccagcagcga tagcgtcgcc
    39001 gattgctttc agcgcctgtt cagcatctgc accctgggcg gtaaagcgta ggcgatgacc
    39061 tttcttaacg ccaagtgcca caactttcat cagactacgt ccgtttgccg gtttgccggt
    39121 accatcaagg tttgtcacgg taatatcact gttaaattgt ttaatggtat tgaccagcat
    39181 ggtacctgga cgagcatgca ggccgtgttc attgcgcacc acaaactccg cgcttaacac
    39241 gtcgtcggtc ggcgcatcat cgctggtcag cagcgccagc aacgttgccg catccgcttt
    39301 cagcaagcgg tcagctttat tgtcgagtaa caaatcagcg agacgcttaa gaaccgcaat
    39361 gggctgatcg tcattcatcg ccacactcac cagcatggct gccgtttcgc cgtccacatc
    39421 aaaagcattt gccgcacggc ttaccgcaat cgcgctacgc agattgcctt cggcgctatc
    39481 gctcagccag ataccctgcc cgaggttcag cggttgttca ttgatggctt tggtgacgaa
    39541 agtggcgtca actgcccccg cttctttcag acgcgcagcg ttcagcgcct gaagagtcag
    39601 cagatcgctg gcgacgatat ccagcgtcag catttcgttg tcgagcttca gctgctcact
    39661 ctgcttttcg cccatcagca atgcgcgaag ttcttctgct gttgttgctg acttcagttg
    39721 ttcagcaacg gaatcatcgc tcagtacgtg ggtcagctgg cgtagcaggc ccagatgttc
    39781 atccgagctg gcagcaatac cgatcgccac gtacgctacc tggccgtcac cccaggtgac
    39841 gccttccggg aactgaaata cctgaacgcc ggttttcagc acctgatcgc gggtgtcggt
    39901 agtgccgtgt ggaatagcaa taccattgcc gaggaacgtt gaggtttgct gttcgcgcgc
    39961 cagcatgcca ttgacgtagc cttcagctac attaccggcc tgcaccagcg ccgcagcgac
    40021 ctggcgaatc gcctcttctt tgtctccggc cttttcgccc ggatggatgt cctgtacgga
    40081 taactggaac atagttctcc tctcttgctg aattgaaacg attcagcttc tatgagaaaa
    40141 aaagcgccaa cctggcttag ggttaaagac aagatcgcgc tgaaacgttt caagagagca
    40201 taatactttt gtttcagcac gcaaggaaag ccgtaaagtt atgagcaaaa gtttgatgtg
    40261 ctgcacaatt ttttgtcatt tttcctaatt gctgatggga aaatccgtta tcagcaattt
    40321 catttcagca gcgtcagcag ccaatcagca acagtgaaat ggcattttga ttattcgtgg
    40381 cgcttttgat ttcgctgtct gtttattttc tgacaagcag cgtaaactcc gcgtcttcct
    40441 cttccagtga tcgaccagca tgcataactc ccccgcagtc accagcgcga aatcgtttga
    40501 cctgacctcg acggcgtttt taatcgttgc ctttctcacc ggtattgcgg gcgctctgca
    40561 aaccccgaca ctcagtattt ttcttaccga tgaagtacat gcccgtccgg cgatggtggg
    40621 attcttcttt accggcagcg ctgtcattgg gattctggtg agtcagtttc tcgccgggcg
    40681 ctctgataag cgcggcgatc gcaaatcgct gattgtcttt tgctgcctgt taggcgtgct
    40741 ggcctgcacc ctttttgcct ggaatcgcaa ctactttgtt ttgctattcg ttggcgtctt
    40801 tcttagcagc tttggctcga ccgctaaccc gcaaatgttt gcccttgccc gtgaacatgc
    40861 cgacaaaacc ggacgtgagg cggtgatgtt cagctctttt ttacgcgctc aggtttcact
    40921 ggcatgggtc attggcccac cgctggctta tgccttagcg atgggtttca gctttacggt
    40981 aatgtatctg agcgcagcgg tagcgtttat tgtttgcggc gtgacggtgt ggctgttttt
    41041 accgtcaatg caaaaagaac ttccgctggc gaccggcacg gttgaagcgc cgcgccgtaa
    41101 ccgtcgcgat acgctgctgc tgtttgtcat ttgtacattg atgtggggct cgaacagcct
    41161 gtacatcatc aacatgccgc tatttattat caacgaactg catcttcccg agaaactggc
    41221 cggtgtgatg atggggaccg ccgccgggct ggaaatcccg accatgttga ttgccggata
    41281 tttcgccaaa cgtctgggta agcgtttctt aatgcgcgtt gctgccgtgg gtggcgtctg
    41341 tttttacgca ggaatgctga tggcgcattc acctgtcatt ctgttgggct tgcagctgct
    41401 aaatgctatt tttattggca ttctgggtgg catcgggatg ctctattttc aggatttgat
    41461 gcccggtcag gcaggttcag ccaccacgct ctataccaat acgtcgcgcg tgggctggat
    41521 catcgcagga tcagtggcgg gcatcgtcgc cgagatctgg aattatcacg ctgtgttctg
    41581 gtttgcgatg gtgatgatta tcgccactct gttttgctta ctgcggatta aagatgttta
    41641 aggcgcggtc agcatttcca gatcgataag ccaggtcatt gcctgctgac gcgagttacc
    41701 gcacatttca gcgctggctt gtaagcctgc acagactttc ggccgcagtg gcgaggtgaa
    41761 aattttacag cgttgctgtt catcaagctg aatgcagggc gtattggcgg gcttgccatc
    41821 tggcataccg ggaatagggc tggaaatcga aggggcagtg caacacgccc cgcaacccgg
    41881 acggcattcc atacgttgtt ctcttaaggt taaccaccgg attgatgagc gcgcacagta
    41941 ccaccttttt tgcaccagca aaagtgcgaa taccacttgc ccgaaaggcc cgtcgcgagt
    42001 actttgtcgc gatatttttg acattttcga ctacaggaat ttttcgatgc caagagcgaa
    42061 cgaaattaaa aaaggtatgg tactgaatta caacggcaaa ctgctgctgg ttaaggacat
    42121 tgatattcag tcgcccactg cccgcggtgc cgctacgctg tacaaaatgc gtttttctga
    42181 tgtccgtacc gggctgaaag tagaagagcg tttcaaagga gatgatatcg ttgacaccgt
    42241 gacgctgacc cgccgttacg ttgacttctc ctatgtcgat ggcaacgaat atgtctttat
    42301 ggataaagaa gactataccc cgtatacctt caccaaagat cagattgaag aagagttgct
    42361 gtttatgcca gagggcggca tgccggacat gcaggtgctg acctgggacg gtcaactgct
    42421 ggcgcttgag ttgccgcaga ccgttgatct ggaaatcgtt gaaaccgcac caggcatcaa
    42481 aggggcatct gccagcgccc gtaacaaacc ggcgacattg agcactggtc tggtgattca
    42541 ggtaccagaa tacttaagcc cgggcgaaaa aattcgtatc catatcgaag aacgccgtta
    42601 tatggggcgt gctgactaac ttcagccgca tgcagaaaag ggatagctca ggctgtccct
    42661 tttttaattt attataccaa tcttctattt tgcgcttcct gaacacctta cgcccgcaat
    42721 tttcactcgt tgattgatcg ccctcacact tcatcgcatt aacaatccag accaatttca
    42781 attgctgtca tataacttta cactgtcatt gttaattaat cgttactaag acgtgactcc
    42841 tatgaataca atcgcctccg ttacgctccc gcatcatgta cacgctccac gctacgatcg
    42901 ccagcagttg caatcacgta tcgttcattt tggctttgga gcctttcacc gcgcacatca
    42961 ggcgttactg accgatcgtg tgctgaatgc ccagagcggc gactggggga tctgtgaaat
    43021 cagcttgttc agcggtgatc aactgatgag ccagctccgc gcacagaacc atttatacac
    43081 cgtgctggag aaaggggcgg acggcaatca ggcgataatt gtcggtgccg ttcacgaatg
    43141 cctgaatgca aaactggatt ccttagcggc aattattgag aaattttgcg agccacaggt
    43201 ggcaattgtt tccctgacga ttaccgaaaa aggctattgt attgacccgg ccaccggtgc
    43261 actcgacacc agcaatccgc ggattattca cgatctacaa acccctgaag aacctcactc
    43321 cgcaccgggt attctcgtcg aagcactgaa acgccgccgt gagcgcggcc ttacaccgtt
    43381 taccgtgctc tcctgcgaca atattcccga caatggccat gtggtgaaaa acgcggtgct
    43441 gggaatggca gaaaaacgtt cgccagaact cgccgggtgg ataaaagagc acgtcagttt
    43501 tccgggaact atggtcgacc gcattgttcc ggcagcaacc gacgaatcac tggcggaaat
    43561 cagccagcat ctgggggtaa atgatccctg cgcgattagc tgcgaaccgt ttatccagtg
    43621 ggtggtggaa gataacttcg tcgctgggcg tcctgcctgg gaagtcgcag gtgtacaaat
    43681 ggtgaatgat gtcctgccat gggaagagat gaaactgcgt atgcttaatg gcagccactc
    43741 ttttctcgct tatctgggtt acctctcagg attcgcccat atcagtgatt gcatgcagga
    43801 tcgcgcattt cgccatgccg ccagagcatt aatgctggat gagcaagcgc cgacactgcg
    43861 aattaaagat gtcgatttaa cacaatatgc ggataagtta attgcacgtt ttgctaaccc
    43921 ggcgctgaaa cataagacct ggcaaatcgc aatggatggt agccagaaat taccgcaacg
    43981 tatgctggca ggtattcgca tacatctggc gcgcgaaacg gactggtcgt tgctggcatt
    44041 aggcgttgca ggctggatgc gttacgtcag cggcgttgat gatgccggaa ataccattga
    44101 tgttcgcgat ccgcttagcg ataaaattcg cgatcttgtt gcgggcagca gcagtgagca
    44161 acgcgtcacc gccctgcttt ccctgcgtga agttttcggt gatgatctgc cagataaccc
    44221 gcattttgtg caggccatcg aacaagcctg gcaacaaatc gcacaattcg gcgcgcatca
    44281 ggcgctatta aacaccctca aaatttaacg atttctgcgg ttaaagcgga tgaagctcgc
    44341 cttcgtccgc tctccccttc tcttttctgc cgtttttagc caggattaac gctcagttaa
    44401 cttttaagag tattgcggag ccgttgtgac caggaccaac ctcatcaccg gttttctcgg
    44461 cagcgggaaa accacgtcga ttcttcatct gttagcccat aaagatccca acgaaaaatg
    44521 ggcggtactg gttaatgaat ttggggaagt cggaattgat ggtgctttgc tcgccgatag
    44581 cggcgcattg ctgaaagaga tccccggcgg ctgcatgtgc tgcgttaatg gtttacccat
    44641 gcaggtagga ttgaatacct tactgcgtca gggaaaacca gaccgcttgt tgatagagcc
    44701 gaccggactg ggtcatccga aacagatcct agatctctta accgcgcccg tgtatgaacc
    44761 gtggatagat ctgcgcgcca cgttgtgcat tctcgatcca cgcctgctgc tggacgaaaa
    44821 aagcgccagc aatgaaaact tccgtgacca gctggctgcc gcagacatta ttgtcgccaa
    44881 taaatccgac cgtgcgacgc ccgaaagtga gcaagcgcta cagcgctggt ggcagcaaaa
    44941 tggtggcgat cggcaattaa ttcacagcga gcatgggaaa attgacggtc atcttctgga
    45001 tttgccgcgt cgcaatttag ccgagttgcc cgccagcgcc gcgcattctc atcagcatgg
    45061 cgtgaaaaaa gggttagcag cgttaagcct gccagagcat caacgctggc gtcgcagtct
    45121 gaacagcggg caaggatatc aggcctgtgg ctggatattc gacgctgata cggtattcga
    45181 caccattggc attctggaat gggcgcgact tgcaccggtg gaacgcgtca aaggcgtgat
    45241 gcgtattccc gaagggctgg tgcgaatcaa ccgtcagggc gatgacctgc acattgaaac
    45301 gcaaaacgtt gcgccaccgg acagtcgtat tgagctgatt tccagcagcg aagctgactg
    45361 gaatgccttg cagagcgcgc tgttgaagct tcgtttagcg accaccgcgt aaggttgcct
    45421 gcgtttttca gtaagataat tagagaaaat atgattaaaa atttgccgca aatagtgttg
    45481 ttgaatattg tcggcctcgc gctgtttctt tcctggtata ttcccgttaa tcatggattc
    45541 tggttgccga ttgatgcgga tattttttat ttttttaatc agaaactggt cgaaagtaag
    45601 gcctttttgt ggctggttgc attgaccaac aatcgcgctt tcgacggttg ttcactgctg
    45661 gcgatgggaa tgttgatgct gagtttctgg ctgaaagaaa acgccccagg cagacgacgt
    45721 atcgtgatta ttggtctggt catgctatta actgcagtgg tattaaacca gctgggtcag
    45781 gcattaattc ctgtaaaacg ggccagccca acattgactt ttaccgatat taaccgcgtc
    45841 agcgaactgc tctctgttcc cacgaaagat gcctcacgag atagctttcc cggcgatcac
    45901 ggtatgatgc tgcttatttt ttcggcattc atgtggcgtt atttcggcaa agttgcaggc
    45961 cttatcgccc ttattatttt tgtggttttt gcgtttccca gagtaatgat tggcgcacac
    46021 tggtttactg acatcattgt cggttcgatg accgtgatat tgatcggttt gccctgggtg
    46081 ttgctgacgc cattaagtga tcgattaatc accttttttg acaaatcact accgggaaaa
    46141 aacaaacatt tccaaaacaa ataactcaca gtaattaaca tcatcagggt tatttttata
    46201 gtgaggataa tcctgatgat gcgcaccgtg ctttcatcca tcgcgcgcaa aaatcattct
    46261 ctaagtaaat gaatggattg catgcgtttc actcaatcgt actttaattg accaatcccg
    46321 tttattaact ttctgtatca ctttttctta taaaaaatca tgtaaaaccg ctcgccaaga
    46381 ccgcaccaat cgggtaatct cgaactcgtt ttgcctcggc ggtagattat cctcacagca
    46441 tataattttg tgcgttagtc cacagatttg gccttaagga attgtttcaa catgcccagg
    46501 taattagtct cgtgtcgctt ggcatttttt tataacgata tttgtcgtta aggacttcaa
    46561 gggaaaacaa acaacatggt caaatctcaa ccgattttga gatatatctt gcgcgggatc
    46621 cccgcgattg cagtagcggt tctgctttct gcatgtagtg caaataacac cgcaaagaat
    46681 atgcatcctg agacacgtgc agtgggtagt gaaacatcat cactgcaagc ttctcaggat
    46741 gaatttgaaa acctggttcg taatgtcgac gtaaaatcgc gaattatgga tcagtatgct
    46801 gactggaaag gtgtacgcta tcgtctgggt ggcagcacta aaaaaggcat cgattgttct
    46861 ggtttcgtac agcgtacatt ccgtgagcaa tttggcttag aacttccgcg ttcgacttac
    46921 gaacagcagg aaatgggtaa atctgtttcc cgtagtaatt tgcgtacggg tgatttagtt
    46981 ctgttccgtg ccggttcaac aggacgccat gtcggtattt atatcggcaa caatcagttt
    47041 gtccatgctt ccaccagcag tggtgttatt atttccagca tgaatgaacc gtactggaag
    47101 aagcgttaca acgaagcacg ccgggttctc agccgcagct aataaaccgt ttggatgcaa
    47161 tcccttggct atcctgacga gttaactgaa agcactgctt aggcagtgct tttttgtttt
    47221 cattcatcag agaaaatgat gtttccgcgt cttgatccag gctatagtcc ggtcattgtt
    47281 atcttttaaa tgttgtcgta atttcaggaa attaacggaa tcatgttcat acgcgctccc
    47341 aattctggac gtaagctcct gcttacctgc attgttgcag gcgtgatgat tgcgatactg
    47401 gtgagctgcc ttcagttttt agtggcctgg cataagcacg aagtcaaata cgacacactg
    47461 attaccgacg tacaaaagta tctcgatacc tattttaccg acctgaaatc cactactgac
    47521 cggctccagc cgctgacctt agatacctgc cagcaggcta accccgaact gactgcccgc
    47581 gcggcgttta gcatgaatgt ccgtacgttt gtgctggtga aagataaaaa aacattctgt
    47641 tcatctgcga ctggtgagat ggacattcca ctaaaagaat tgattccggc gctcgacatt
    47701 aataaaaatg tcgatatggc gatcttaccc ggtacgccga tggtgccgaa caaacccgca
    47761 atcgtcatct ggtatcgcaa cccgttgttg aaaaatagtg gcgtgtttgc cgcgctgaat
    47821 ctcaatctga cgccttctct cttttatagt tcacggcagg aagattacga tggcgtcgcc
    47881 ctcattattg gtaatactgc gctatctacc ttttcttcac gtttgatgaa cgttaatgaa
    47941 ttaaccgaca tgccggtccg tgaaactaaa attgcgggca ttcctctgac cgttcggctt
    48001 tatgcagatg actggacatg gaacgatgtg tggtacgcat ttttactggg tggcatgagt
    48061 ggaactttcg ttggacttct ctgctattac ctgatgagtg tgcgtatgcg cccaggcaga
    48121 gaaatcatga ccgccatcaa gcgcgaacaa ttttacgtgg tgtatcaacc ggtggtggat
    48181 acacaagctt tgcgggtaac gggcctggaa gtactgctac gctggcggca tccagtagca
    48241 ggagaaatcc ccccggatgc cttcattaac tttgccgaag cgcaaaagat gattgtgcca
    48301 ctgactcagc acctgtttga gttgattgcc cgcgatgccg cagaattaga aaaagtactg
    48361 ccggtaggcg tcaaatttgg cattaacatt gcgccggccc acttgcacag cgaaagcttt
    48421 aaagcggata tccagaaact gctcacttcc ctgcccgcac accatttcca gattgtgctg
    48481 gaaattaccg agcgcgatat gctgaaagag cgagaagcca cacaactctt cgcctggctg
    48541 cattcggtcg gcgtagaaat tgctattgat gacttcggca ccgggcacag cgcgcttatc
    48601 tatcttgagc gttttacgct cgattatctg aaaattgatc gtggatttat caacgccatc
    48661 ggtacggaaa cgatcacttc acccgtactt gacgcggtgc tgacgctggc gaaacgcctc
    48721 aatatgctga cggttgctga aggggtcgaa acgccagaac aggcacgatg gctaagcgaa
    48781 cgcggcgtta atttcatgca aggctactgg attagtcgcc cgttaccgct ggacgatttt
    48841 gttcgctggc tgaagaaacc gtatacgccg cagtggtaag gtgtgcttac gtcccttatt
    48901 attcatagtg aaagcatgcc ggattgcggc taatgatgag taaaaggaaa tccgttgcag
    48961 atgattgtgc gcatactgct gctgtttatc gctctgttca cctttggtgc gcaggcgcag
    49021 actatcaagg aaagctatgc ctttgccgta ctgggcgaac cccggtacgc atttaatttc
    49081 aaccattttg attatgtgaa ccccgccgcg ccaaaaggtg ggcaaataac gttgtctgcg
    49141 ctcggcacct tcgataattt caaccgctat gcactgcgcg gcaatgcggg cgcacgcacc
    49201 gagcagctgt acgacacgct atttacgact tccgatgacg aaccaggcag ttattacccg
    49261 ctgattgctg aaagcgcacg ctatgctgac gattattcct gggtggaggt cgctattaat
    49321 ccacgcgccc gttttcatga tggttcgccc attactgccc gcgatgtaga gtttactttt
    49381 caaaaattta tgaccgaagg cgtgccgcaa tttcgtctgg tctacaaagg caccaccgtc
    49441 aaagccattg cgccgttaac cgtgcgcatt gagttagcga aacccggcaa agaagatatg
    49501 ctgagcctgt tttcgctgcc ggtctttcca gaaaagtact ggaaggatca caaacttagc
    49561 gacccgctcg ccacgcctcc gcttgccagt ggtccgtacc gaattacgtc ctggaaaatg
    49621 gggcaaaata ttgtctattc ccgcgtgaaa gattactggg cagcaaactt accggtaaac
    49681 cgtggacgct ggaatttcga caccattcgc tacgattatt acctcgatga taatgtcgcc
    49741 tttgaagcgt ttaaagccgg tgcctttgat ttgcgtttgg aaaacgacgc caaaaactgg
    49801 gtcacgcgct ataccggtaa aaatttcgat aagaaataca tcatcaaaga tgagcaaaag
    49861 aacgaatcag cccaggatac gcgctggctg gcgtttaata tccaacgtcc ggtattcagc
    49921 gatcgccggg tgcgggaagc aatcactctc gcctttgact ttgaatggat gaacaaggcg
    49981 ttgttttata atgcctggag tcgcacgaac agttattttc agaataccga atatgcggcc
    50041 agaaattacc ccgacgccgc ggagctggtg cttctggcac caatgaaaaa agatctaccg
    50101 ccagaagtct tcacgcaaat ctaccagccg ccggtatcca aaggcgatgg ctacgatcgt
    50161 gacaacctgt taaaagccga caaacttctc aacgaagcgg gctgggtgct gaagggtcag
    50221 caacgcgtaa atgccacaac gggtcagccg ctcagctttg agttattgct accttcgagc
    50281 agcaatagtc agtgggtatt gccgttccag cacagcctgc aacggctggg tatcaacatg
    50341 gacattcgca aagtggataa ctcgcaaatc accaaccgca tgcgcagtcg cgactatgac
    50401 atgatgccgc gcgtatggcg ggcgatgccg tggcccagtt ccgatttaca gatttcctgg
    50461 tcatcggaat atatcaattc cacttataat gcccccggcg tgcaaagccc ggttatcgac
    50521 tcgctgatca accaaattat tgccgcgcag ggaaataaag aaaaattact gccgttgggg
    50581 cgagcactgg atcgcgtatt aacgtggaat tattacatgc tgccaatgtg gtacatggcg
    50641 gaagaccgtc tcgcctggtg ggataaattc tcccaacccg ctgtacgccc tgtttacagc
    50701 ctgggtattg atacctggtg gtatgacgtt aataaagcga cgaaactgcc gtcagccaga
    50761 caacagggag agtagatggg cgcttatctg attcgccgtc tgttgctggt gatcccaacg
    50821 ttatgggcga ttatcactat caactttttc atcgtgcaaa ttgcgcctgg cggtccggtc
    50881 gaccaggcca tcgccgccat tgagtttggt aatactggtg tattacccgg tgcaggcggt
    50941 gaaggtgttc gcgccagcca tgcacaaacg ggcgtcggta atatcagcga cagtaattac
    51001 cgtggcggac gcggattgga tccagaagtg atcgccgaga tcactcatcg ctacggtttc
    51061 gataagccga tccacgaacg ttacttcaaa atgctctggg actacatccg ttttgatttt
    51121 ggcgacagcc tttttcgcag cgcctcggtg ctgacgctga ttaaagacag tctgccggtc
    51181 tccatcaccc tcggattgtg gagcacgctg attatctatc tggtgtcgat tccgttaggc
    51241 attcgcaaag ctgtttataa tgggagccgt tttgacgtct ggagtagtgc atttatcatc
    51301 atcggctacg ccattccggc ctttttgttt gccatcctgc tgattgtctt cttcgcgggc
    51361 ggcagctatt tcgacctgtt ccctctgcgc ggcctggttt ccgctaactt tgattcgctg
    51421 ccgtggtatc agaaaatcac cgattatctg tggcatatca cgctgccggt gctggcgaca
    51481 gtgattggcg gctttgcggc tctgaccatg ctgacaaaaa actcattcct tgatgaagtg
    51541 cgtaagcaat acgtggtgac ggcacgtgcg aaaggggtaa gtgaaaaaaa tattctctgg
    51601 aaacatgtgt tccgcaacgc catgctgctg gtgattgccg gttttccggc gacgtttatc
    51661 agcatgtttt ttaccggctc gctgctgatt gaggtgatgt tttcactcaa tggtcttggc
    51721 ttactgggct acgaagcgac cgtctcgcgc gattatcctg taatgtttgg taccttgtat
    51781 attttcaccc tgattggcct gctgctgaat attgtcagtg atatcagcta tacgctggtc
    51841 gatccgcgta tagattttga gggacgttaa tgtcgcgact cagccccgtc aatcaggccc
    51901 gttgggcgcg ttttcgtcat aaccgtcgcg gctactggtc gttatggatt ttcctcgtct
    51961 tgtttggttt gagtttgtgt tctgaactta tcgccaacga taaaccgttg ctggtgcgtt
    52021 atgacggcag ttggtatttc ccgttgttga aaaactacag cgaaagcgat tttggcggcc
    52081 cgctggcaag tcaggctgat tatcaggacc cgtggctgaa acaacggctg gaaaataacg
    52141 gctgggtact gtgggcaccg attcgctttg gtgctaccag tatcaacttt gctaccgata
    52201 agcccttccc ttctccaccc tcccggcaaa actggctggg aacggatgcc aatggcggcg
    52261 atgtgctggc gcgcattctc tatggcacgc ggatctcggt tctgtttggc ctgatgctga
    52321 ctctctgttc cagcgtgatg ggcgtgctgg cgggggcgct acaaggctat tacggcggta
    52381 aagtcgatct ctgggggcaa cgctttattg aagtatggtc agggatgccg acgctgtttt
    52441 tgattatttt actttccagc gtcgtacagc ctaacttctg gtggctgctg gcaattaccg
    52501 tcttgtttgg ctggatgagt ctggtcggcg tggtgcgggc ggagttttta cgtacccgta
    52561 atttcgacta catccgcgcg gcgcaggcgc ttggcgtcag cgatcgcagt atcatcctgc
    52621 gtcatatgtt gccaaatgcc atggtcgcta ccctcacctt tttaccgttt attttatgta
    52681 gttcgataac caccctgacc tcactcgatt tcctcggctt cggtctgccg ctcggttcac
    52741 cgtcactcgg cgaactgctg ttacaaggga aaaataacct tcaggccccg tggcttggga
    52801 tcaccgcctt cttgtcggtg gcgatattgt tgtctttgct gatctttatt ggtgaagccg
    52861 tccgcgacgc atttgatcct aataaggcgg tgtagcatga cgcaaactct gttagcgatt
    52921 gaaaatttgt cggtgggttt tcgccatcag caaaccgtac gcacagtagt caatgatgtt
    52981 tcacttcaga ttgaggctgg cgaaacgctg gcgctggtgg gtgagtcagg ttcaggcaaa
    53041 agcgttaccg cgctgtcaat tttacgcctg ctcccttccc cgccggttga atatctctcc
    53101 ggcgatattc gttttcatgg cgaatcgctg cttcatgcca gcgatcaaac gttgcgcggt
    53161 gtacgcggta ataagatcgc catgattttt caggagccga tggtgtcgtt aaatccatta
    53221 cataccctgg aaaaacagct ttatgaagta ctttcactcc accgcgggat gcgtcgggaa
    53281 gcggctcgtg gcgaaattct taactgcctt gatcgcgtcg gtatccgcca ggcggcaaaa
    53341 cggctgacag attatccgca tcagctctcc ggcggcgaac gccagcgggt gatgattgcg
    53401 atggcgctgt taacgcgacc ggaattatta attgccgatg aaccaaccac cgcgctggac
    53461 gtctctgtcc aggcgcagat tttacagctg ttgcgcgaac tgcaaggcga gctgaatatg
    53521 ggtatgctgt ttattactca taacctcagc attgttagaa aactggccca ccgcgtggcg
    53581 gtaatgcaaa acggtcgctg tgtcgagcaa aataacgccg ctacgctatt tgcctcaccc
    53641 actcatcctt acacacaaaa gctactcaac agtgaaccgt caggcgaccc ggtcccattg
    53701 ccagaacctg cctcaacgtt gctggatgtt gaacagcttc aggttgcctt ccccattcgc
    53761 aaagggattt tgaagcgcat tgtggatcat aatgtggtgg tgaaaaacat cagttttacg
    53821 ctacgagcgg gtgaaacact gggtttagtg ggcgagtctg gttccgggaa aagcacgacg
    53881 ggactggcgc tgctgcgact gattaactct cagggcagca tcgtctttga cggtaagcca
    53941 ctgcaaaatt taaatcgccg ccagctgtta cctattcgtc atcgcattca ggtggtattt
    54001 caggatccaa actcctcgct caacccacga ctcaacgttt tgcagattat tgaggaaggc
    54061 ttacgggttc accagccgac gctttctgcc gcgcaacgcg aacaacaagt gatagccgtg
    54121 atgcatgaag tgggattaga tcctgaaaca cgccaccgtt atccggcgga gttctctggt
    54181 ggtcagcggc aacgtattgc gattgccagg gcgttaattc ttaagccctc gctgatcatt
    54241 cttgatgaac caacatcatc actcgacaaa acggtgcagg cgcaaatatt gacgctattg
    54301 aaatcattgc aacaaaagca tcaactggcc tatttgttta tcagccacga tttgcacgtt
    54361 gtccgcgcgt tatgtcatca ggttatcgta ctgcgacaag gggaagtagt ggaacaagga
    54421 ccgtgcgcgc gcgtgtttgc cgcaccgcag caggagtata cgcgtcagct actggcgttg
    54481 agctgacgct taaaaaggat tgtagtttga aaagggttcg gcaattgcca caccaaaatt
    54541 tttcaatcgg catgttgcag caaattcgtc ctggcgattt acaaacaggc acggctcacc
    54601 ttcacactcc agcaccgaac atggaacttc gatgtcgctt aacgcctggc ttagtttgta
    54661 catcaccgac catgcattat caccatccgg gctaagcaat ttaagcgcca ccaggctgtt
    54721 gtcaccgcac ggtccatttt gcggaatcgg ttcaaccttc gaacctgcga aaccagcaga
    54781 ccagcgatag ttctgcggca gtcgatggac gattgagagt tgtaatgaag tcacttattt
    54841 tccccggaag tacattttac ttcacaattt gtttacatca attttaacac atcatcaaca
    54901 atccgtcttt atacagatcg taaagattga tgcctcgcct gcgtggcctc tatggctctg
    54961 atttaaataa tatctgcggg cgtggtcacg tttgcggggc tatatgtacc cgtttctgcg
    55021 atctaactca acctttttaa ctacaatgat gtgacttttt acataaattg attttacata
    55081 aaataaacat atatcggggg aatgatagat ttgtggttga catgcatcaa caaaggaagc
    55141 cttttagctt cctcgttgtg caatagatca ccgttttttc ggccgactgg cgtacaaaca
    55201 gaagagaatg gagctggttg cgcagaatgc aattgaccaa atcatcggcc atgcagagtt
    55261 aaaggtcgca agagaaagta atgcgccaac aattgcccct atgccgaaac ggaaggttcc
    55321 tgccagcgaa gatgccgttc ccgccatatg gggaaactca tcaagaatga ccgccatcgc
    55381 attggatgac accatcgaca cgcagcccac aaacgccgca acgccaacca ccagcgacca
    55441 gaatcccagc cccagcagtg cactgatgac catccacgct gccataataa actgtatcca
    55501 caaccccgag cggaacatat ttaacgcgcc aatacggcgg acgaagcggc tgttaaagat
    55561 ggtcatcacg aacagaaaaa gaatgtttag cgcaaagtaa taaccaaagt tttccggcgc
    55621 gacgtggttg atttcaatgt agacaaacgg tccggcgctt aagaatgaga acatcccggc
    55681 aaagctgaaa ccgctggcca gcatataact caggacacgc ttatgacgga acagcgccgc
    55741 aaagttacca atagtggtac gaatgtgaaa aggctggcga cgctccggtg gtaaggtttc
    55801 tttgatcaga aagaagatca ttgccgaagc cagaatcgcc gccacggcga ggatccagaa
    55861 gatgtaatgc cagctcagcc acaccagcac ccagccgcca actatcggtg ccatcagcgg
    55921 tgcaatggtc gtcaccagca tgacaaacga catcatccgt gagaactctt ctttcgggta
    55981 gatatcacgc atcaaagcgt tgataaccac gctggccgca gccgcagcca gcccgtggaa
    56041 gaaacgcatc acaatcagct gatcgatggt ttgtgccaac gcacacgcca ccgcggcggc
    56101 ggcaaacacc agcgtaccgc caagaaccac cggcttacgc ccgaagctgt ctgccatcgg
    56161 cccgtagatt aactgcccca acgcaaagcc cagaatataa gtactgaggg tcatctgcgt
    56221 actgcccgcc ggtacaccaa actgagctga aattaccggt agcgcaggca gatacatatc
    56281 aatcgacagc ggcatcaaca tggccagcag gccaaggata aaaacaatag caaacgacga
    56341 atgctgtcgg gtggtcacaa cgggctcctg aaagtcattg aaaagttaga cgacgctggc
    56401 aatttcttct tcagttaacg gacgatattc accgggagct aaatcagcat ccagcgtaat
    56461 gccgccaata cgttcacgat gcagctcaac cacatggtta cccacggcgg cgaacatgcg
    56521 tttcacctga tgataacgcc cttcgctgat ggtcagacga acctgcgttg gggtaatcac
    56581 ttccagcacc gccggcttag tgagatcttt ttcgttatgc agctgcacgc ctttagcaaa
    56641 ttgctctgcc gtatcgtcag ctacgggtga ttccagcgtc accagatagg tcttctcgca
    56701 atgatggcgc ggggaagtaa tgcggtgcga ccactgacca tcatctgtca ttagtaccag
    56761 accggtggta tcgatatcca gacgccctgc agcgtgcagt ttccacgcta ccggttcatc
    56821 aagaaaatag agcaccgttg ggtgatccgg gtcgtccgtg gagcaaacat agccctgagg
    56881 cttattgagc atgaagtaac gtggaccgtg ttgctgcgcc agcgggttgc catcgtaagc
    56941 gacatcatgt tcagggagca gtttgaacgc tgcattacgg acgatttcgc catcgacggt
    57001 gacacgattg ccgcggattt cacgcccggc aatagcacgg ctaacgccga gttgctgtgc
    57061 gataaattta tcaagtcgca tgtgtgtgat tttgcctgta aaaaacggag gtcgggtaat
    57121 gacccgaaaa tctgaactgt tgtctgccca gtatagcggt ctaacaacat ccctcaaggg
    57181 aaaaagatcc atggcatact attagcagaa taatctacct acgcgagacc atgattttta
    57241 cacttcgccc atatcagcaa gaagccgtgg atgccacgct caaccatttt cgtcgtcata
    57301 aaacccctgc cgttatcgtg ctgcccaccg gtgcaggtaa aagcctggtg atagcggaac
    57361 tggcgcggct ggcgcgtggt cgcgtgctgg tgctggcaca cgttaaagaa ctggtggcgc
    57421 aaaaccatgc aaaatatcag gcgctggggc tggaagccga tatttttgcc gccgggctaa
    57481 agcgcaaaga gagccacggt aaagtggtat ttggcagcgt gcagtcggtc gcccgtaatc
    57541 ttgatgcctt tcagggtgaa ttttcgctgt tgattgtcga tgaatgtcac cgtattggtg
    57601 acgatgaaga gagccagtat cagcaaatcc tcactcacct gaccaaagtg aatccccact
    57661 tacgcctgct ggggctgact gccacgcctt ttcgactggg caaaggctgg atttatcagt
    57721 tccattatca cggcatggta cgcggcgatg agaaagccct tttccgtgac tgcatttatg
    57781 agctaccgct gcgttatatg attaaacacg gctatctgac gccgccagaa cgactggata
    57841 tgccagtagt gcaatacgat ttcagccgct tgcaggcaca gagtaacggg ctattcagcg
    57901 aagccgatct caaccgcgag ctgaaaaaac agcaacgcat taccccgcac atcatcagcc
    57961 agattatgga gtttgctgta acgcgcaaag gggtgatgat tttcgccgcc accgttgaac
    58021 acgcaaaaga gattgtggga ctgctgcctg ccgaagatgc tgcactgatt actggcgaca
    58081 cccccggcgc tgagcgcgat gtgttaattg aagattttaa agcccagcgt tttcgctatc
    58141 tggtcaacgt cgcggtactg accaccggat ttgacgcccc gcacgtcgat cttatcgcca
    58201 ttctgcgccc taccgaatca gtgagtcttt accaacaaat tgtcgggcgc ggtctgcgtc
    58261 tcgctcccgg caagactgat tgcttaattc ttgattatgc gggtaaccct cacgatctct
    58321 acgcgccgga agttggtaca ccgaaaggca aaagtgacaa cgttccggta caggttttct
    58381 gccctgcctg cggttttgcc aacacctttt gggggaaaac gaccgccgac gggacattga
    58441 ttgaacactt tggtcgtcgc tgtcagggat ggtttgaaga tgacgacgga catcgcgaac
    58501 agtgtgactt ccgtttccgt tttaaaaatt gcccgcaatg taacgctgaa aatgatattg
    58561 ccgcccgccg ctgccgcgaa tgtgacacgg tactggttga tccggatgat atgttaaaag
    58621 cggcgctacg actgaaagac gcgctggtat tacgctgtag cggcatgtct ttgcagcatg
    58681 ggcacgacga gaaaggcgaa tggttgaaaa tcacctatta cgatgaagac ggcgcggatg
    58741 tgagtgagcg tttccgtctg caaacgcccg cccagcgtac cgccttcgaa caacttttta
    58801 tccgtccgca tacgcgcaca ccgggcatcc ctctgcgctg gatcaccgcc gccgatatcc
    58861 tcgcccagca agcattactg cgacacccgg attttgtcgt cgcccgcatg aaaggtcagt
    58921 actggcaagt gcgtgaaaaa gtgttcgatt acgaaggtcg ttttcgtcgg gcgcacgaat
    58981 tacgcggtta atggtagttt tgattgatgt ataaggcgat tgagtataga atctcgcccg
    59041 cttttgcata cgcaaagcag atcacttacc tgttgctggg tcgcctgtag caggattaat
    59101 ttaagagaga aagaaatgtt tactatcaac gcagaagtac gtaaagagca gggtaagggt
    59161 gcgagccgcc gcctgcgtgc cgctaacaag ttcccggcaa tcatctacgg tggcaaagaa
    59221 gcgccgctgg ctgtcgaact ggatcacgac aaagtcatga acatgcaagt taaagctgaa
    59281 ttctacagcg aagttctgac catcgttgtt gacggtaaag aaatcaaagt taaagctcag
    59341 gacgtacagc gtcacccgta caaaccgaag ctgctgcaca tcgacttcgt tcgcgcttaa
    59401 ttgctgaata agttgtaaaa aaccccgctc cggcggggtt ttttgtctct gcaaattatg
    59461 cctgattacg gtattgctat tttttgcagg ccagataagg cgttcacgcc gcatctggca
    59521 ttcagaacac aaaaccgatt aattgccgcc agacgtccgg cgctgcaatt gatcgcgcaa
    59581 gttcggcggc gtgcctttaa tggtcagggt atcggttgct ggatcccaga agatgcgttc
    59641 acctaacagc atcgcatcaa agttaatagt caagccaccg ccgctaccgg caaacttcgt
    59701 caactgacgc agtgtgctac ggtctgccgg gaagctctct tccagttcgt agcctttttc
    59761 agccgcaaac tccgtgaagc tcacttcgct gacgcctgcc agctctttcg acagcgattc
    59821 cagttcaatc tcttcacctg cctgcagctg ctcattacag tagctataaa cctgctggcg
    59881 cacgttctgt cgctctgctt tatccagttg tgcttcggca gtgaaatcat cgaccgcttg
    59941 caacagccca cggttttgcg ctttcgcgtt taacccttcg ctggcaccga ggaaatccat
    60001 aaagaaatcc gccactttgc gccctactcg ccctttcagg aaagtgagat agcgggtcga
    60061 ctctggattg gtttcccact cggttaaatc gatacgcgca acaatatccg catgattgat
    60121 atcaagataa tgggttgggt tgatgtcgag attttcgtta acacgcatac tgctcaggtt
    60181 gctcagaact gccaccagca gatactccac cgccagatag cgatagtggc aaaacagcac
    60241 gaatccaccg tcagcaaaag gatatttcgc taattcgtca cgcaggcgac cggttgccgc
    60301 gcggctaaat gccagaaagt cctcttcgcc ctgacgctgt aagcgcagcg tctgcgccag
    60361 ttcactctct tcgctaaaca agccgtaagc tttatttttg gcgctataga cccgatgcag
    60421 ttctgccacc atctcaacga cggtttccgt cggttccagc aatgaatcgc gcaacaccag
    60481 ctcaaggttc tgctcatcac gcttgataag ctggtgcaga gcaatctggt tgatatccag
    60541 actcatgata aactctcctt taagaccggg cggtattcaa ccaccgccgg ttaagcgacg
    60601 caataaaaga tggcggtcat tttacagaag gcggcgtaac gagaatgatt taacgcataa
    60661 aaaagcagaa aaaaaaccgt tgctacggta atatgttgcc ctttcatcaa ctaactgatt
    60721 tcgatttatg ccacaaattt cccgctacag tgatgaacag gttgaacaat tgctcgcaga
    60781 gctgctcaac gtactggaaa aacataaggc tccgaccgac ctttccctga tggtgttagg
    60841 caacatggtc actaacctta tcaacaccag cattgccccg gcccaacgcc aggcaattgc
    60901 caactctttt gcccgcgcct tacagtcctc tatcaacgaa gacaaagcgc actaagggaa
    60961 acagataaca ggttatggta actcatcgtc agcgctaccg tgaaaaagtc tcccagatgg
    61021 tcagttgggg gcactggttt gcactgttca atattctgct ttcgctcgtc attggcagcc
    61081 gttacctgtt tatcgccgac tggccgacaa cgcttgctgg tcgcatttat tcctacgtaa
    61141 gcattatcgg ccatttcagc ttcctggtgt tcgccaccta cttgctgatc ctcttcccgc
    61201 tgacctttat cgtcggctcc cagaggctga tgaggttttt gtccgtcatt ctggcaacgg
    61261 cgggaatgac gctattactg atcgatagcg aagtctttac tcgtttccat ctccatctta
    61321 atcccatcgt ctggcaactg gttatcaacc cagacgaaaa tgagatggcg cgcgactggc
    61381 agctgatgtt catcagcgtg ccggttattt tattgcttga actggtgttt gcgacgtgga
    61441 gctggcaaaa gctacgcagc ctgacgcgtc gtcggcgctt cgcgcgcccg ctggccgcat
    61501 tcttatttat cgcctttatc gcctcgcatg tggtttatat ctgggccgat gccaacttct
    61561 atcgcccgat caccatgcaa cgcgctaacc tgccgctttc gtacccgatg acggcgcgac
    61621 gttttcttga gaagcatggt ctgcttgatg cgcaggagta tcaacgccgt ctcattgagc
    61681 aaggtaatcc agacgccgtt tccgttcagt atccgttaag cgaactgcgc tatcgcgata
    61741 tgggcaccgg gcagaatgtg ctgttgatta ctgtcgatgg cctgaactac tcacgcttcg
    61801 agaagcagat gcctgcgctg gcaggttttg ctgagcaaaa tatttcgttc acgcgccata
    61861 tgagctccgg caacactaca gacaacggca tctttggcct gttctatggc atctcgccga
    61921 gctatatgga tggcattctg tcgacccgta cgcctgcggc attaattact gcgcttaatc
    61981 agcaaggcta tcagctgggg ttattctcgt cagatggctt taccagcccg ctgtatcggc
    62041 aggcattgtt gtcagatttc tcgatgccga gtgtacgcac ccaatccgac gaacagaccg
    62101 ccacgcagtg gatcaactgg ctgggccgct acgcacaaga agataaccgc tggttctcgt
    62161 gggtctcttt caatggcaca aacattgacg acagcaatca gcaggcattt gcacggaaat
    62221 atagccgggc ggcaggcaat gtcgatgacc agatcaaccg cgtgctcaat gcactgcgtg
    62281 attctggcaa actggacaat acggtggtga ttatcactgc cgggcggggt attccgctga
    62341 gcgaagagga agaaaccttt gactggtccc acggtcatct gcaggtgcca ttagtgattc
    62401 actggccagg cacgccggcg cagcgtatta atgcgctgac tgatcatacc gatctgatga
    62461 cgacgctgat gcaacgcctg ctacatgtca gcacacctgc cagcgaatat tcgcaaggtc
    62521 aggatttgtt caaccctcaa cgccgccatt actgggttac cgccgcggat aacgatacgc
    62581 tggcaattac caccccgaaa aagacactgg tgctgaacaa taacggtaaa taccgcactt
    62641 acaacttacg tggtgaaaga gtgaaagatg aaaaaccaca gttaagtttg ttattgcaag
    62701 tactgacaga cgagaagcgt tttatcgcta actgattaat tataaatcag ttagcaaaat
    62761 atcttacttg caatcggtgt ggaaaacggt agtattagca gccacgagtc ggcacgtagc
    62821 gcagcctggt agcgcaccgt catggggtgt cgggggtcgg aggttcaaat cctctcgtgc
    62881 cgaccaaaaa tcccaagaaa accaacctat tgcggttggt ttttttatat ctgcatttaa
    62941 ttcgataaac agacagcgac acatcacgac ctgtttattt tctgttatca gaacgtccag
    63001 actacacccg cctgagttgt gctgctttct tcacgggaga aacgactatc gataccgagc
    63061 gtcaggcccg tcaatgtttg ctcaaagcca gctcccgcat cagtgttcac gttgttgcgg
    63121 ctgttaattg ccgaactcca catcgccgcc ctttacgcta ccaagacgct cacgcacggt
    63181 gtccagttct gcatcaaata ccagcggttg tgctgccgcc atattcagca catccgtggt
    63241 tgaagggggc atttgtacgc gattctctgt gagactccag ctatggttgc cattatccag
    63301 caaggtatat tcatacgtac tgatatcaac aacgcttccg gcattgccca acgtaaatgc
    63361 cgcatcaccg cgccctcctt taatcgtggg atcagtcgcc gcgccataaa cctgtagcac
    63421 tccattattg atcgtagtct ggtttaccgc gccacccatt tgaacaattt gataaccatc
    63481 atttaatact gagtattctg cggtgccatt tatttcaatg tactgatatc ccccgttaat
    63541 tttagtatta ctacttacac caaattctta tatatgctgt tctccgccag gattgatttt
    63601 cgtattattg gcggccccat aaaccagctg gatatctttt tccaggacaa caccatcaac
    63661 tgtctcacca tactcaacag ttgatgccaa tgctatgggt gaatataacg ctgtagccac
    63721 cagaacagat attgcggaac gacaaagaga aacagaacca gattggtgca ttaagctttc
    63781 atcctatgaa attaattgct gttaaaagca ttggggacag aaaataccca tagctccata
    63841 cccggagtca gttttaaaaa actgtttaaa gaaatgcaca agtattgtga ttgatttttt
    63901 agttgttttt cctgatgaga agctgatgca aaattccgtc tttataatga agatgatgcc
    63961 aaagcgaacg acaaagttgt agttttcact acatgtccat acataaaatg gggtaacatt
    64021 cacgcgcctg gtagcgttac caacgctacg ctcaaacata atgattctaa taaaacctca
    64081 ggagactact atgcctgaag caacgccttt tcaggtgatg attgtggatg atcatccact
    64141 tatgcgacgc ggtgttcgtc agttactgga gcttgatcct ggctttgaag tggtcgccga
    64201 agcgggcgac ggcgcgagcg cgatcgatct ggcgaataga ctggatatcg acgtgatctt
    64261 gctggatctc aatatgaaag gtatgagtgg cctggatacc ctcaatgcct tgcgcaggga
    64321 tggcgttacc gcgcaaatta ttatcctgac cgtatccgat gcctccagcg atgtctttgc
    64381 gctgatagac gcaggcgcag acggttatct gttgaaagac agcgacccgg aagtgttgct
    64441 tgaagcgatt cgtgccggag cgaaaggcag caaagttttt agcgaacgcg tcaatcagta
    64501 cttacgtgaa cgtgaaatgt ttggcgcgga agaagatccc ttcagcgtgc tgacggagcg
    64561 cgagctggat gttctgcacg agctggcaca ggggctgtca aataaacaga ttgcctcggt
    64621 gttgaatatt tccgagcaga cagtaaaagt acacattcgc aatctgctgc gtaaactcaa
    64681 tgtccgctca cgcgtggcgg ccaccattct gttcctgcaa caacgcgggg cacaataaaa
    64741 atagcccgat ggcatcacca tcgggccact ttttatttac tctcctgcgg cgacaaatgt
    64801 tgcatcgcct gcgcgatact acgttcgatc accgcacggc gagtatcgtt ggcaggtaag
    64861 agtttcaaca tcatttccca cgcggcaacg gcttcgccaa atcgttgctg ctcaaaggca
    64921 ttaaatgcat acatgcttaa cacacggata ttgctatggt ccgttctcac caactggcgc
    64981 agcagttcgc cgccgaggcg gttgtcgttg ggatcagatg aacgtgtcaa cgcttcagcg
    65041 tatccgagcg ccgcatcact gttcttcgga tcgaggcgat atgcagtagc gtatgcatcg
    65101 gtggcgatac tggcgttacc cagcgccatg ccaacgcggc ccaacataat ccagccttct
    65161 atatctcccg gatttttttg cagttgagta cgcatcccca gcgcaaggcg cgacatctct
    65221 tcttcgttga gcggagcggc tttcggatcc agcgccctgt ccagtaacgc cggagcctgc
    65281 gcggtggcct gctgccagat tttcacctgc tgataattgc cagtctggta gtagctgacg
    65341 ccagccacaa ttaacgccac cacaataccc ggcagataaa caacatatcc ggcacgctta
    65401 ccttccggca cgctttgttc tggaaacgct tccggcacca cgcgtacccg acgccgcgaa
    65461 cgggcgtata tgacccaacc gccaatgcca atagccacta ctggcagcac ccacagcagc
    65521 acggtcagcg gcgttaacgg cggatcgtaa gtgacgaagt tgccgtaacg cgccaccata
    65581 taatcgacaa tctctttctt acttttgcct tcctgcatca gttcatacac tttctggcgc
    65641 aggtcggtgg caatcatcga gttggaatcg gcaatgctgt tgttctggca tttcgggcag
    65701 cgcagttctt cagtgagctg acggaactgt tgttcctgtg cttcatcttt aaactgcaac
    65761 acgtcgatgg tcgccagcgc tgagccggag atcatcagca tcagcacgcc caataaaaac
    65821 ctcattgtgc ggcctcctta ctgtatttct cccacagtgg cttgatctct tcttcccaga
    65881 cgcgaggatt gagatcgccc gcatggcgat agcgaatgat gccgttgccg tcaataagga
    65941 acgtttctgg cgcgccatag acaccgagat ccagccctaa catgccgtcg ccatcaaaca
    66001 ggcttagtgc gtaaggattg cccaactctt tcagccagct gattgccttc tggcgatcgt
    66061 ctttatagtt catgccgacc acgcggatgc cctgcgcaga aagctgattc agatattgat
    66121 gttccgcacg gcaggtcgga caccaggtcg cccagacgtt aagcagcacc ggtttgccct
    66181 gagtcagcac atccgcctga taaaactgcc ccggattgtc cagtgattcg aggcgaaact
    66241 tcggcactag cttgccaatg agcgccgatt ccagattggt cggatcatcc ccttcggcat
    66301 tacgcgccag ctgccacagt agcgccgcgg caatcgccag gaagataatc aacggaatta
    66361 acaatacttt gcgcttcata cagcctccgg cgcagttttc tgcgggttca cgcgcttacg
    66421 atagcgagga tcaaacagg
//
LOCUS       DAZGHF010000008        65857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGHF010000008 DAZGHF010000000
VERSION     DAZGHF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.008"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..468)
                     /gene="folK"
                     /locus_tag="ACHK3A_000596"
     CDS             complement(<1..468)
                     /gene="folK"
                     /locus_tag="ACHK3A_000596"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO8738730.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELLFPDGLSLVEALQAKGFNEL"
     gene            complement(465..1862)
                     /gene="pcnB"
                     /locus_tag="ACHK3A_000597"
     CDS             complement(465..1862)
                     /gene="pcnB"
                     /locus_tag="ACHK3A_000597"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO8738731.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1922..2818)
                     /gene="gluQRS"
                     /locus_tag="ACHK3A_000598"
     CDS             complement(1922..2818)
                     /gene="gluQRS"
                     /locus_tag="ACHK3A_000598"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO8738732.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTLFTDQLRGIIHANEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNTQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2885..3340)
                     /gene="dksA"
                     /locus_tag="ACHK3A_000599"
     CDS             complement(2885..3340)
                     /gene="dksA"
                     /locus_tag="ACHK3A_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO8738733.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3518..4222)
                     /gene="sfsA"
                     /locus_tag="ACHK3A_000600"
     CDS             complement(3518..4222)
                     /gene="sfsA"
                     /locus_tag="ACHK3A_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO8738734.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTSC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4237..4767)
                     /gene="thpR"
                     /locus_tag="ACHK3A_000601"
     CDS             complement(4237..4767)
                     /gene="thpR"
                     /locus_tag="ACHK3A_000601"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO8738735.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4841..7270
                     /gene="hrpB"
                     /locus_tag="ACHK3A_000602"
     CDS             4841..7270
                     /gene="hrpB"
                     /locus_tag="ACHK3A_000602"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO8738736.1"
                     /translation="MSSLPVAAVLPELLAALDCASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVAHFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDTAQMSWLDQPPTVNLQAAKRL
                     LQMLGALEGERLSAQGQKMAVLGSDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIACRRGQDG
                     RYQLANGMGAMLDADDALSRYEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLFAAKWLPEYDWPAVDDESLLATLETWLLPHMSGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDKPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7364..9895
                     /gene="mrcB"
                     /locus_tag="ACHK3A_000603"
     CDS             7364..9895
                     /gene="mrcB"
                     /locus_tag="ACHK3A_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO8738737.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDYDDYDDYEDEEP
                     MPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLP
                     AAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDF
                     PDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRS
                     GFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLF
                     LSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYF
                     GRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDM
                     LSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAE
                     KAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIG
                     SLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVD
                     ALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAF
                     QTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTG
                     RQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMS
                     IYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEM
                     QQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10115..12304
                     /gene="fhuA"
                     /locus_tag="ACHK3A_000604"
     CDS             10115..12304
                     /gene="fhuA"
                     /locus_tag="ACHK3A_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO8738738.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIASDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12355..13152
                     /gene="fhuC"
                     /locus_tag="ACHK3A_000605"
     CDS             12355..13152
                     /gene="fhuC"
                     /locus_tag="ACHK3A_000605"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO8738739.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13152..14042
                     /gene="fhuD"
                     /locus_tag="ACHK3A_000606"
     CDS             13152..14042
                     /gene="fhuD"
                     /locus_tag="ACHK3A_000606"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO8738740.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAIIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMIW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14039..16021
                     /gene="fhuB"
                     /locus_tag="ACHK3A_000607"
     CDS             14039..16021
                     /gene="fhuB"
                     /locus_tag="ACHK3A_000607"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO8738741.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAINPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16056..17336)
                     /gene="hemL"
                     /locus_tag="ACHK3A_000608"
     CDS             complement(16056..17336)
                     /gene="hemL"
                     /locus_tag="ACHK3A_000608"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO8738742.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPHEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17502..17549
                     /gene="yadX"
                     /locus_tag="ACHK3A_000609"
     CDS             17502..17549
                     /gene="yadX"
                     /locus_tag="ACHK3A_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO8738743.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17561..18982
                     /gene="clcA"
                     /locus_tag="ACHK3A_000610"
     CDS             17561..18982
                     /gene="clcA"
                     /locus_tag="ACHK3A_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO8738744.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19006..19071
                     /gene="yadW"
                     /locus_tag="ACHK3A_000611"
     CDS             19006..19071
                     /gene="yadW"
                     /locus_tag="ACHK3A_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO8738745.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19064..19408
                     /gene="erpA"
                     /locus_tag="ACHK3A_000612"
     CDS             19064..19408
                     /gene="erpA"
                     /locus_tag="ACHK3A_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO8738746.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19455..20078)
                     /gene="yadS"
                     /locus_tag="ACHK3A_000613"
     CDS             complement(19455..20078)
                     /gene="yadS"
                     /locus_tag="ACHK3A_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO8738747.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20116..20916)
                     /gene="btuF"
                     /locus_tag="ACHK3A_000614"
     CDS             complement(20116..20916)
                     /gene="btuF"
                     /locus_tag="ACHK3A_000614"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO8738748.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLVPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20909..21607)
                     /gene="mtnN"
                     /locus_tag="ACHK3A_000615"
     CDS             complement(20909..21607)
                     /gene="mtnN"
                     /locus_tag="ACHK3A_000615"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO8738749.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21691..23208
                     /gene="dgt"
                     /locus_tag="ACHK3A_000616"
     CDS             21691..23208
                     /gene="dgt"
                     /locus_tag="ACHK3A_000616"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO8738750.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLYSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23338..24762
                     /gene="degP"
                     /locus_tag="ACHK3A_000617"
     CDS             23338..24762
                     /gene="degP"
                     /locus_tag="ACHK3A_000617"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO8738751.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24917..26074
                     /gene="cdaR"
                     /locus_tag="ACHK3A_000618"
     CDS             24917..26074
                     /gene="cdaR"
                     /locus_tag="ACHK3A_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO8738752.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEQR"
     gene            complement(26189..26575)
                     /gene="yaeH"
                     /locus_tag="ACHK3A_000619"
     CDS             complement(26189..26575)
                     /gene="yaeH"
                     /locus_tag="ACHK3A_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO8738753.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGIGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26733..27545)
                     /gene="yaeI"
                     /locus_tag="ACHK3A_000620"
     CDS             complement(26733..27545)
                     /gene="yaeI"
                     /locus_tag="ACHK3A_000620"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO8738754.1"
                     /translation="MITRRRFLQAAAATLAAGSSLSYMHYWEPGWLELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGVAQKPDLILLGGDYVLFDMQLNFSAFS
                     DVLSSLAECAPTFACYGNHDRPVGTEKNRLIGEALKSAGITVLFNEAMLITTQKQQFE
                     LVGTGDLWTGQCKPPPASEANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIP
                     LVGEPFAPIEDKRYAAGLNAFGERQIYTTRGVGSLYGLRLNCRPEVTILELV"
     gene            complement(27598..28422)
                     /gene="dapD"
                     /locus_tag="ACHK3A_000621"
     CDS             complement(27598..28422)
                     /gene="dapD"
                     /locus_tag="ACHK3A_000621"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO8738755.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28453..31125)
                     /gene="glnD"
                     /locus_tag="ACHK3A_000622"
     CDS             complement(28453..31125)
                     /gene="glnD"
                     /locus_tag="ACHK3A_000622"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO8738756.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31187..31981)
                     /gene="map"
                     /locus_tag="ACHK3A_000623"
     CDS             complement(31187..31981)
                     /gene="map"
                     /locus_tag="ACHK3A_000623"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO8738757.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32349..33074
                     /gene="rpsB"
                     /locus_tag="ACHK3A_000624"
     CDS             32349..33074
                     /gene="rpsB"
                     /locus_tag="ACHK3A_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO8738758.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33332..34183
                     /gene="tsf"
                     /locus_tag="ACHK3A_000625"
     CDS             33332..34183
                     /gene="tsf"
                     /locus_tag="ACHK3A_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO8738759.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34330..35055
                     /gene="pyrH"
                     /locus_tag="ACHK3A_000626"
     CDS             34330..35055
                     /gene="pyrH"
                     /locus_tag="ACHK3A_000626"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO8738760.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35347..35904
                     /gene="frr"
                     /locus_tag="ACHK3A_000627"
     CDS             35347..35904
                     /gene="frr"
                     /locus_tag="ACHK3A_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO8738761.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35996..37192
                     /gene="ispC"
                     /locus_tag="ACHK3A_000628"
     CDS             35996..37192
                     /gene="ispC"
                     /locus_tag="ACHK3A_000628"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO8738762.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37381..38139
                     /gene="ispU"
                     /locus_tag="ACHK3A_000629"
                     /gene_synonym="uppS"
     CDS             37381..38139
                     /gene="ispU"
                     /locus_tag="ACHK3A_000629"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO8738763.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38152..39009
                     /gene="cdsA"
                     /locus_tag="ACHK3A_000630"
     CDS             38152..39009
                     /gene="cdsA"
                     /locus_tag="ACHK3A_000630"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO8738764.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39021..40373
                     /gene="rseP"
                     /locus_tag="ACHK3A_000631"
     CDS             39021..40373
                     /gene="rseP"
                     /locus_tag="ACHK3A_000631"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO8738765.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVTSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40403..42835
                     /gene="bamA"
                     /locus_tag="ACHK3A_000632"
     CDS             40403..42835
                     /gene="bamA"
                     /locus_tag="ACHK3A_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO8738766.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42957..43442
                     /gene="skp"
                     /locus_tag="ACHK3A_000633"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42957..43442
                     /gene="skp"
                     /locus_tag="ACHK3A_000633"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO8738767.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43446..44471
                     /gene="lpxD"
                     /locus_tag="ACHK3A_000634"
     CDS             43446..44471
                     /gene="lpxD"
                     /locus_tag="ACHK3A_000634"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO8738768.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44576..45031
                     /gene="fabZ"
                     /locus_tag="ACHK3A_000635"
     CDS             44576..45031
                     /gene="fabZ"
                     /locus_tag="ACHK3A_000635"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO8738769.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45035..45823
                     /gene="lpxA"
                     /locus_tag="ACHK3A_000636"
     CDS             45035..45823
                     /gene="lpxA"
                     /locus_tag="ACHK3A_000636"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO8738770.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45823..46971
                     /gene="lpxB"
                     /locus_tag="ACHK3A_000637"
     CDS             45823..46971
                     /gene="lpxB"
                     /locus_tag="ACHK3A_000637"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO8738771.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTTRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46968..47564
                     /gene="rnhB"
                     /locus_tag="ACHK3A_000638"
     CDS             46968..47564
                     /gene="rnhB"
                     /locus_tag="ACHK3A_000638"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO8738772.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRVLGLAS"
     gene            47601..51083
                     /gene="dnaE"
                     /locus_tag="ACHK3A_000639"
     CDS             47601..51083
                     /gene="dnaE"
                     /locus_tag="ACHK3A_000639"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO8738773.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51096..52055
                     /gene="accA"
                     /locus_tag="ACHK3A_000640"
     CDS             51096..52055
                     /gene="accA"
                     /locus_tag="ACHK3A_000640"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO8738774.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52154..54295
                     /gene="ldcC"
                     /locus_tag="ACHK3A_000641"
     CDS             52154..54295
                     /gene="ldcC"
                     /locus_tag="ACHK3A_000641"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO8738775.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGERWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54352..54741
                     /gene="yaeR"
                     /locus_tag="ACHK3A_000642"
     CDS             54352..54741
                     /gene="yaeR"
                     /locus_tag="ACHK3A_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO8738776.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54806..56104
                     /gene="tilS"
                     /locus_tag="ACHK3A_000643"
     CDS             54806..56104
                     /gene="tilS"
                     /locus_tag="ACHK3A_000643"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO8738777.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRMENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIIGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56153..56413)
                     /gene="rof"
                     /locus_tag="ACHK3A_000644"
     CDS             complement(56153..56413)
                     /gene="rof"
                     /locus_tag="ACHK3A_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO8738778.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGTTRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56400..56600)
                     /gene="yaeP"
                     /locus_tag="ACHK3A_000645"
     CDS             complement(56400..56600)
                     /gene="yaeP"
                     /locus_tag="ACHK3A_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO8738779.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56766..57311
                     /gene="yaeQ"
                     /locus_tag="ACHK3A_000646"
     CDS             56766..57311
                     /gene="yaeQ"
                     /locus_tag="ACHK3A_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO8738780.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFSNLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57308..57730
                     /gene="arfB"
                     /locus_tag="ACHK3A_000647"
     CDS             57308..57730
                     /gene="arfB"
                     /locus_tag="ACHK3A_000647"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO8738781.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57744..58454
                     /gene="nlpE"
                     /locus_tag="ACHK3A_000648"
                     /gene_synonym="cutF"
     CDS             57744..58454
                     /gene="nlpE"
                     /locus_tag="ACHK3A_000648"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414734.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO8738782.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58484..59308)
                     /gene="yaeF"
                     /locus_tag="ACHK3A_000649"
     CDS             complement(58484..59308)
                     /gene="yaeF"
                     /locus_tag="ACHK3A_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO8738783.1"
                     /translation="MDKPKAHCRLLLPCFLFLSACTVDISQPDSSATAVDVEAKTWVV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQVCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(59361..61079)
                     /gene="proS"
                     /locus_tag="ACHK3A_000650"
     CDS             complement(59361..61079)
                     /gene="proS"
                     /locus_tag="ACHK3A_000650"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO8738784.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61190..61897)
                     /gene="tsaA"
                     /locus_tag="ACHK3A_000651"
     CDS             complement(61190..61897)
                     /gene="tsaA"
                     /locus_tag="ACHK3A_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO8738785.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61894..62298)
                     /gene="rcsF"
                     /locus_tag="ACHK3A_000652"
     CDS             complement(61894..62298)
                     /gene="rcsF"
                     /locus_tag="ACHK3A_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO8738786.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAESAKPKVP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62416..63231)
                     /gene="metQ"
                     /locus_tag="ACHK3A_000653"
     CDS             complement(62416..63231)
                     /gene="metQ"
                     /locus_tag="ACHK3A_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO8738787.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63271..63924)
                     /gene="metI"
                     /locus_tag="ACHK3A_000654"
     CDS             complement(63271..63924)
                     /gene="metI"
                     /locus_tag="ACHK3A_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO8738788.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63917..64948)
                     /gene="metN"
                     /locus_tag="ACHK3A_000655"
     CDS             complement(63917..64948)
                     /gene="metN"
                     /locus_tag="ACHK3A_000655"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO8738789.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAITWLQEHH
                     VKVEVLGYV"
     gene            65136..65708
                     /gene="gmhB"
                     /locus_tag="ACHK3A_000656"
     CDS             65136..65708
                     /gene="gmhB"
                     /locus_tag="ACHK3A_000656"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO8738790.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15653 a        16300 c        18079 g        15825 t
ORIGIN      
        1 taattcatta aagccttttg cctgtagagc ttcaactaat gataagccat caggaaatag
       61 caattccggc gcaatttcaa acagtggcca cagcataaag ccgcgatttt tcatatcgta
      121 gtgcggaacg gtcagacgtt cggtatttat tacttcatta ccaaacagca tgatgtcgag
      181 atccagcgtg cgcggtcccc agcgttcagc tttacggacg cgaccttgct gcaattcgat
      241 acgctgtgtg tgattgagta gctcttcagg ggcaagagtg gttttcagcg cgacggctgc
      301 atttaagtaa tcgggttgat cttgcggccc cagcggtggg gtgcggtaaa acgaagaaac
      361 agcaagaatg cggctttctg gaatatcgcc taatgctttc agggcagcat tgacctgctc
      421 cagcggagag gccagattgc tgcctatggc aatatacgcc actgtcatgc ggtaccctca
      481 cgacgcggtg cgcgtttgcg tggacgacga gtacggcgac gcggtgacgg ttcttcatcc
      541 agttcgttaa gcatcccttt ttggtctggt ggcgcggaaa cctggaactc accccaccat
      601 ttcaccagac gttgcagttc agcgttacgc tcaacttcgg ctcgtaaggc caacaggtcg
      661 tatgccgcac ggaacttagg atgctccagc agtttccagg cgcgtttacc ctgacgtcgg
      721 gacatacgca actgcaactg ccagatatcg cgggttaacg tcgtcagacg tttcgggatc
      781 gccagtgaac gacaggcttc gtccagcacg tcgttcatcg ccagcgcgaa agcgtcgtga
      841 taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct ccagcagtgg gtaccagaac
      901 atggcggcaa acaggaacgc cgggttcacg cgcatatcgt tatggatacg cgtatcggta
      961 ttcttcagca cctgttcaat gatccgctcc atcgggctgt cgccattttc cgtgaagtag
     1021 cgggtaatgg tcgggaacag cggctggaac aggtgatatt cacacagcag cttataggtt
     1081 tcgtaaccgt agcccgcttg cagcagtttc aaagattctt caaacagacg tgccggtgga
     1141 atatcgttca gcagggtagc caggcgaggg atcggttctg cggtttcagg actgatacgc
     1201 atacccaatt tggcggcaaa acgtaccgcg cgcagcatcc gtaccggatc ttcacggtag
     1261 cgcgtttccg ggttaccaat cagacggata acgccgtcct tcagatcctt catgccgcca
     1321 acgtaatcac ggacggtaaa atctgccacg ctgtaataca ggctgttgat agtgaaatcg
     1381 cggcgctggg cgtcttcttc gatggagccg aaaatgttgt cgcgcagcaa catgccgttt
     1441 tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt cgtgatgtcc acggaaggtc
     1501 gcaacttcga taatctccgg gccaaacatc acatgagcca gacggaaacg gcgacccacc
     1561 aggcggcagt tacggaacag tttacgtacc tgttcaggcg tggcgttagt ggttacgtca
     1621 aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc caccgccaac cagccaggct
     1681 tcgtatcccg ctttattgag cctgtacatt accttcaggg cattttcact gatatctttg
     1741 cgggaaatag catgctgctc acgcgggatc accgtcacct gtggacgggc gactgcctgt
     1801 tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa aattagcgac tcgggtaaaa
     1861 atagtacacc tcggtagtgt caaacatcat tcaggacaaa aaaatagcgg ctaatcatag
     1921 ctcagcatga cgcatttgag aatgttgaat ttacaattgc cgactcaggc acggcggtaa
     1981 gccgccagtt tttgacggct gactgaagga tttgctcgac gctgaaatcc tgccagtgtg
     2041 cttctgcctg ctgacccaga aattgaagtg ccgcgattag taccgggcgc ggatcgcctt
     2101 tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc gccttgtgtg ttaagcgcca
     2161 gcggtaaatg aatgtaatct ggcactttcc agccaaaaag ctggtacagc gagatttgcc
     2221 gcactgtcgg ttcgatcaaa tctgccccgc ggacaatttc gctcacgccc tggaagtgat
     2281 catccaccac caccgccagg ttataggcaa acaaaccatc acggcgatga ataataaaat
     2341 cttcccgcgc cagtttttcg ttggcgtgaa taatgccgcg cagctgatca gtaaatagcg
     2401 tgaccggatg ctgctggcgg atacgtactg cggcgttgtc tggtccatga tgcaattccc
     2461 ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg cgcgcgcgtg caggtgcagt
     2521 aataacttag tccttgctca tgtaaccagg cgagtgcttc gcgataggca tcgtgacgtt
     2581 gcgattgcca gagaacgtcg ccgtcccagt gcagaccgta atgttccagc tggcgcagga
     2641 tagtctctgc ggcaccggga acttcacgag gcgggtcgat atcttcaatg cgtaccagcc
     2701 agcgaccttg ccgggcgcga gcctgtaaat agctgccaag cgcggcaatc agagagccaa
     2761 aatgaagctc gccggaagga gagggggcaa agcggccaat atagtgtgtg tctgtcatct
     2821 cgttgaacaa aaaataaggc gggagcattt cccgcctgtg gtaaacgtga tggaacggct
     2881 gtatttagcc agccatttgt ttttcgcgaa tttcagccag cgttttgcag tcgatgcaca
     2941 gatcggctgt cgggcgcgct tccagacggc gaataccaat ttcaacaccg caggattcgc
     3001 agtagccgaa atcttcgtct tccacttttt tcagcgtctt ctcgatcttt ttgatcagct
     3061 tacgctcgcg atcgcggtta cgcagttcga ggctgaactc ttcttcctgg gctgcacggt
     3121 ctaccgggtc cgggaagttg gctgcttcat cctgcatatg tgtaacggtg cgatcgactt
     3181 catccctgag ttgattacgc catgcttcca gaatacgacg gaagtgcgcc agctgggctt
     3241 cattcatata ctcttcgccc ggcttctcct gatatggttc caccccagcg atggcgagaa
     3301 tactcaggga cgatgtttta cggttttgcc cttcttgcat gttgcttctc cttaacacgc
     3361 actatcgatc cccatgttcg ggggaaaaat gaggccgcta taaatagcag atgcttttcc
     3421 ggatagcaat tatctaaacg taacacttga caactgtgtg aggaaaagcg tatttgcgca
     3481 cgcgaccaga atgtaaatta accagttaca tactttacta caatgtaacc ggcagtgatt
     3541 ttttaagagc catgccttca gcagaaagtt ccgctttgta agccagaatt tctacccccc
     3601 tctgttgagc ttctgacaat agttgcgcgt atttctcatc gatgtggcgc gcgggtgaaa
     3661 accgtgtaat ggctgaatgc agcacggcga aaaagataac cgcacgctgg ccttcagccg
     3721 ctacgctcat caactcccga aggtgtttct gacctcgttc agtgaccgca tcgggaaaat
     3781 atccctgttc gttctccgct aacgtaaccg atttcacttc aatatagcag tctggacgcg
     3841 aatccgcctg caacataaag tcaatacggc tgcgttcggc gccgtatttt acttcgcttt
     3901 tcagcgagct atagcctgac agctctgaaa ttgattcatt aaggatagcc tctttcgtca
     3961 acctgttagc ccaaagcgtg ttgacgcaaa taaatgcgcc gctctggctt tgagtcaatt
     4021 cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt cgaataccag acggtatcgc
     4081 caggcgttgc acaactggtc atcgcacccg tattcgggca gtgtagcgtt aattcgcgac
     4141 catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg ctgaattagc gtcgcgcgct
     4201 gtagaggggg agaaaattcc attacgacaa tccttgttat tgcgttagcg cccagcgttt
     4261 tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag gcgtaaaggg tgaactccgt
     4321 caccgtatac gaccagttaa aacctggcgg cgggattgtc accgcctcgc tggcgtcgcg
     4381 caataaggta atatgtggat gaaacggacg attgctttga aaacaaccgc tgcgggcagc
     4441 ctgtgaacgg agcatattcg ccagctggat taagccgcgt ggtggctgac gcatccctaa
     4501 ccacaccaca cgcgaacgca gccattgacc ggcgtcatca agcgtgagtg tgaaacccgg
     4561 ttgacgaatc cgcccggcta aaagagaaag cgccttctct ttctcggcgc tcacttcgcc
     4621 taaaaatgcc agagtcagat gcaaattatc ggcggcgacc ggacgtcccg cgtcaggtgg
     4681 gaagtgtgtg gcgcgccagt ggataatctg ttcgcggatt tctgcaggta agtcgatagc
     4741 aaagaacagc cgttgcggtt cagacatgtg aggcactcgg ttatgaatta ccgcgatgct
     4801 acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt gtgtcgtcgt tgcccgttgc
     4861 tgccgtctta cctgagttac ttgctgctct cgattgtgca tcgcaggtgt tattaagtgc
     4921 gccgaccggg gccgggaaat caacctggct gccgctgcaa ctgctggcgc atcccggcat
     4981 taacgggaaa attatcctgc tggagccgcg tcgtctggcg gcgcgtaacg ttgcgcaacg
     5041 actggcggag ctgcttaacg aaaagccagg cgataccgtt ggctaccgga tgcgtgcgca
     5101 aaactgcgtc gggccgaata cccgcctgga agtggttacc gaaggcgtgc tgacgcggat
     5161 gatccagcgt gacccggaac tgagcggtgt tggattggtg atccttgatg aatttcatga
     5221 gcgcagcttg caggcggatt tggcgttggc gctgttactc gatgtgcaac aaggtctgcg
     5281 tgatgacctt aaactgctga ttatgtcggc tacgctggac aacgaccgct tgcagcaaat
     5341 gctgccagaa gcgcccgtcg tcatctcaga agggcgctcg tttccggttg aacgccgtta
     5401 tttgccgctg cccgctcatc agcgttttga cgaagccgtc gcggttgcca ccgccgaaat
     5461 gctgcgtcag gaaagtggat cattactgtt atttttacct ggcgtcggag aaattcagcg
     5521 tgtgcaggaa caactggctt cacgcatcgg cagtgatgtg ttgctctgcc cgctgtatgg
     5581 cgcgttgtcg ctgaacgatc agcgaaaagc gatcctcccg gcaccgcaag ggatgcgtaa
     5641 agtggtgctg gcgaccaata ttgctgaaac cagtttaacc atcgaaggta ttcgtctggt
     5701 ggtggattgt gcccaggagc gtgtggcgca ttttgatccg cgtacagggc ttacgcggct
     5761 ggttactcaa cgcgttagtc aggcgtcgat gacgcaacgt gccgggcgtg ccgggcgtct
     5821 ggagccgggt atctgcctgc atttaatcgc caaagaacaa gcagaacgcg ctgccgcgca
     5881 aagtgaaccg gagatcttac aaagcgatct ttccggtttg ctgatggaat tactgcaatg
     5941 gggatgcagc gatacggcgc agatgagctg gctggatcaa ccgccaacgg tgaatctaca
     6001 ggccgcgaaa cggctgttac aaatgctggg ggcgctggag ggtgaacggc ttagcgcaca
     6061 agggcaaaaa atggcagtgc tgggtagcga tccgcgttta gcggcaatgc tggttagcgc
     6121 gaagaacgac gacgaagctg ctaccgcggc aaaaattgcc gccattctcg aagagccgcc
     6181 acggatgggc aatagtgacc tgggcgtggc gttttcgcgc aatcaacccg cctggcagca
     6241 acgtagtcag caactgttaa aacgcttaaa cgtacgcggc ggtgaggcag acagttcgct
     6301 tatcgcgccg ctacttgccg gggcgtatgc cgatcgcatt gcttgtcgcc gtgggcaaga
     6361 tggacgctat caactggcaa acggcatggg ggcgatgctc gatgccgacg atgcgctaag
     6421 ccgctacgaa tggttgatcg caccgttatt attgcagggc agcgcctcgc cggatgcgcg
     6481 gattttactg gcgctaccgg tcgatatcga tgaattagta caacgctgtc cgcagctggt
     6541 acagcagtcg gacaccgtgg aatgggatga cgcgcaaggc acgctgaaag cctggcgtcg
     6601 gctgcaaatc ggtcagttga cggtgaaagt gcagccactg gcgaaaccct cggaagacga
     6661 gttgcatcag gcgatgctta acggcatccg tgataaaggt ttaagcgtgc tcaactggac
     6721 ggcggaagcg gaacagctac gcttgcgttt gttattcgcc gcaaagtggt tgccggaata
     6781 tgactggcca gcggttgatg atgagagttt gttggcgacg ctggaaacgt ggctactgcc
     6841 gcatatgagc ggcgtgcatt cactacgcgg cctgaaatca ctcgacattt atcaggcgct
     6901 acgcggatta cttgattggg gaatgcagca acgtctggat agtgaattgc ctgcgcatta
     6961 cactgtgccg acgggaagcc ggatcgccat tcgttatcat gaagataaac cgcccgcgct
     7021 ggcggtgaga atgcaggaga tgtttggtga ggccaccaat ccgacgatcg cccaggggcg
     7081 cgtgccgctg gtgctggagt tgctttcccc tgcccaaagg ccattacaaa tcacacgaga
     7141 tttgagcgcc ttctggaaag gagcgtaccg tgaggtgcaa aaagagatga aagggcgtta
     7201 tcccaaacat gtctggccgg acgacccggc aaatactgca ccgacgcgac ggacgaaaaa
     7261 gtattcgtga agaccaaagc aaatttgaga gatatcttct tctgtcttgc aacagaagaa
     7321 cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa agcatggccg ggaatgaccg
     7381 cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg taagccgtcg
     7441 tcgttacgaa gatgacgatt acgacgatta tgatgactat gaggatgaag aaccgatgcc
     7501 gcgcaaaggt aagggcaaag gcaaagggcg taagcctcgt ggcaaacgcg gctggctatg
     7561 gctgctgcta aaactggcta tcgtttttgc cgtgctgatc gccatttacg gcgtttatct
     7621 cgatcaaaaa attcgcagcc gtattgatgg caaggtctgg caattgcccg cggcagttta
     7681 tggccgaatg gtcaaccttg agccagacat gaccatcagc aagaacgaaa tggtgaagct
     7741 gctggaggcg acccagtatc gtcaggtgtc gaaaatgacc cgtcctggcg aatttaccgt
     7801 gcaggccaac agcattgaga tgatccgccg tccgtttgat ttcccggaca gtaaagaagg
     7861 gcaggtgcgt gcgcgtctga cctttgatgg cgatcatctg gcgacgatcg tcaatatgga
     7921 gaacaaccgt cagtttggtt tcttccgtct tgatccgcgc ctgatcacca tgatctcctc
     7981 gccaaacggt gagcagcgtt tgtttgtgcc gcgcagtggt ttcccggatt tgctggtgga
     8041 tactttgctg gcgacagaag accgccattt ctacgagcat gatgggatca gtctctactc
     8101 catcggacgt gcggtgctgg caaacctgac cgccggacgc acggtgcagg gtgcgagtac
     8161 gctgacgcaa cagctggtga aaaacctgtt cctctccagc gagcgctctt actggcgtaa
     8221 agcgaacgaa gcttacatgg cgctgatcat ggacgcgcgt tacagcaaag accgtattct
     8281 tgagctgtat atgaacgagg tgtatctcgg tcagagcggc gacaacgaaa tccgcggctt
     8341 cccgctggcg agcttgtatt actttggtcg cccagtagaa gaattgagcc tcgaccagca
     8401 ggcgctgttg gtgggtatgg tgaaaggggc gtccatctac aatccgtggc gtaacccgaa
     8461 actggcgttg gagcgacgta atctggtact gcgtctgctg caacagcaac agattattga
     8521 tcaagaactc tatgacatgt tgagtgcacg tccgctgggg gttcagccgc gcggtggggt
     8581 gatctctcca cagccagcct ttatgcaact ggtgcgtcag gagctgcagg caaaactggg
     8641 cgataaggta aaagatctct ccggcgtgaa gatcttcact acctttgact cggtggccca
     8701 ggacgcggca gaaaaagccg ccgtggaagg cattccggca ctgaagaaac agcgtaagtt
     8761 gagcgatctt gaaaccgcga ttgtggtcgt cgaccgcttt agtggtgaag ttcgtgcgat
     8821 ggttggaggt tctgagccgc agtttgcggg ctacaaccgt gcgatgcagg cgcgtcgttc
     8881 gattggttcc cttgcaaaac cagcgactta tctgacggcc ttaagccagc cgaaaatcta
     8941 tcgcctgaac acgtggattg cggatgcgcc aatcgcgcta cgtcagccga atggccaggt
     9001 ctggtcaccg cagaacgatg accgtcgtta tagcgaaagc ggcagagtga tgctggtaga
     9061 tgcgttgacc cgttcgatga acgtgccgac ggtgaatctg ggcatggcgt tggggctgcc
     9121 tgcggttacg gagacctgga ttaaactggg tgtgccgaaa gatcaactga atccagttcc
     9181 agcaatgttg ttgggggcgt tgaacttaac gccaatcgaa gtggcgcagg cgttccagac
     9241 catcgccagc ggtggtaacc gtgctccgct ttctgcactg cgttcggtaa tcgcggaaga
     9301 tggcaaggtg ctgtatcaga gcttcccgca ggcggaacgc gctgttccgg cgcaggcggc
     9361 gtatctgaca ctatggacca tgcagcaggt ggtacaacgt ggtacggggc gtcagcttgg
     9421 ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg actaccaaca ataacgtaga
     9481 tacctggttt gcgggcattg acggcagtac ggtgaccatc acctgggtag gccgtgataa
     9541 caaccaaccg accaaattgt atggagccag cggggcaatg tcgatttatc agcgttatct
     9601 ggctaaccag acgccaacac cgctgaatct tgttccgcca gaagatattg cagatatggg
     9661 cgtggactat gacggcaact ttgtttgcag cggtggcatg cgtgtcttgc cggtctggac
     9721 cagcgatccg caatcgctgt gccagcagag tgagatgcag cagcagccgt caggcaatcc
     9781 gtttgatcag tcttctcagc cgcagcaaca gccgcaacag caacctgctc agcaagagca
     9841 gaaagacagc gacggtgtag ccggttggat caaggatatg tttggtagta attaacgtct
     9901 aagcgtgaca taccggatgg cgagtagcca tccggtaaaa taacatccca cccaaagtat
     9961 taaccctttc ttttcatctg gttgtttatt aacccttcag gaaagctcag attgcgtacc
    10021 gcttgcgaac ccgccagcgt ttcgaatatt atcttatctt tataataatc attctcgttt
    10081 acgttatcat tcactttata tcagagatat accaatggcg cgttccaaaa ctgctcagcc
    10141 aaaacactca ctgcgtaaaa tcgcagttgt agtagccaca gcggttagcg gcatgtctgt
    10201 ttatgcacag gcagcggttg aaccgaaaga agacactatc accgttaccg ctgcacctgc
    10261 gccgcaagaa agcgcatggg gaccggctgc aactattgcg gcgaagcact ctgctactgc
    10321 gactaaaacg gatacaccaa ttgaaaaaac gccgcagtct atttcggttg tgacaaatga
    10381 agagatgcag atgcatcaat ttcagtctgt aaaagaagca ttaggttata cacctggcgt
    10441 tacggttagc agtcgtggtg cttctaatac atatgatttt gttattattc gtggtttctc
    10501 atctgttggt ctgaatcaaa ataattacct tgatggacta aaacttcagg gcaactttta
    10561 taacgatgct gtgattgatc cttacatgct cgagagggtt gaactgatgc gcgggccgac
    10621 gtctgttctc tatgggaaaa gtaatcctgg tgggattatt tctatggtga gtaagcgtcc
    10681 gacaactgaa cccctgaaag aaattcagtt taaaatgggg acggataatc tgtttcagac
    10741 cggattcgat ttcagtgatg cgctggatga taacggtgag ttctcctacc gtttgactgg
    10801 cctcgcacgc tcaacaaacg aacagcagaa aaactctgaa tctcagcgtt ataccattgc
    10861 tccatcattc tcatggcgtc cagacgacaa aaccaatttt actttcctgt cctattttca
    10921 gaacgaacca gaaacgggtt attacggttg gttgccgaaa gaggggaccg ttgagccatt
    10981 gcctaatggt aagcgtctac cgactgactt caatgaaggt gcatcgaata atacatactc
    11041 ccgtaaccag aaaatggtgg gatatagttt tgaacatggt tttaatgaca ccttcaccgt
    11101 gcgtcagaat ctgcgtttca gtgaaatgaa aacctcacag aaaagtgttt atggcacagg
    11161 gattgccagc gatggtcata ccctaaaccg cgggacagtg gtggataatg agcgtctgca
    11221 aaactttagc gttgataccc aacttgaaag taaatttgct acaggtgaag tcgagcatac
    11281 tttgctgaca ggggtagact tcatgcgtat gcgcaatgat atcaatgcca gctttggatc
    11341 tgcaccatcc atcgatcttt ataacaaata tcatcctgaa tactttgcat ttggtaacgc
    11401 agagccatac caaatgaatg aaagcaaaca aacaggtatt tatgttcagg atcaggcgga
    11461 atggaataaa tgggtattca ctctgggggg acgctacgat tggtctaagc aagcgaccac
    11521 tgttcgtgaa aactcttata cgccgactga aggttatatt gagcgtaatg atcatcagtt
    11581 cacctggcgc ggtggtgtaa actacttatt cgataatggt atttcacctt actttagtta
    11641 tagccagtcc tttgaaccga gtgctttcga tctgtggagc aacccgcgcg tttcctataa
    11701 gccatcgaaa ggtgaacagt atgaagctgg cgtaaaatat gttccgaatg atatgccggt
    11761 cgttgttacg ggcgcagtct atcaattgac gaaaacaaat aacctgacag cagacccaac
    11821 aaacccgtta gcgcaagtcc cagcaggtga gattcgcgct cgtggtgtgg aacttgaagc
    11881 aaaagcggcg ttaaatgcca atattaactt gacggcttct tatacctaca cagatgcgga
    11941 atacaccaaa gacaccaatc tcaaaggtaa aactccagaa caagtaccgg agcatatggc
    12001 atctctctgg ggggattata ccttcaatga agggccgctt tctggtttaa cattgggaac
    12061 aggtggtcgt tttattggtt ccagctatgg tgatccggcg aacactttta aagtgggtag
    12121 cgcagctgta atggatgctg ttgtaaaata tgatctggca cgctttggta tggcgggatc
    12181 cagccttgct gtgaacgtca acaatttgct cgatcgtgag tatgttgcca gttgcttcca
    12241 gacctatggc tgcttctggg gcgcagaacg tcaggtcgtt gcaaccgcaa ccttccgttt
    12301 ctaatttttt ctttggggca cggatttccg tgcccgcttc acaagttggc tgttatgcag
    12361 gaatacacga atcattccga taccactttt gcactgcgta atatctcctt tcgtgtgcca
    12421 gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg ccgggaaagt gactggtttg
    12481 attggtcaca acggttctgg taaatccact ctgctcaaaa tgcttggccg tcatcagcca
    12541 ccgtcggaag gcgaaattct tcttgatgcc cagccgctgg aaagctggag cagcaaagcg
    12601 ttcgcccgca aagtggctta tttgccgcag cagcttcctc cggcagaagg gatgaccgtg
    12661 cgtgaactgg tggcgattgg tcgttacccg tggcatggcg cactggggcg ctttggtgcg
    12721 gcagatcgcg aaaaggtcga agaggcgatt tcgctggttg gcttaaaacc gctggcgcat
    12781 cggctggtcg atagtctctc tggcggcgaa cgtcagcggg cgtggatcgc tatgctggtg
    12841 gcgcaggata gccgttgtct gttgctcgac gaaccgacct cggcgctgga tatcgcccac
    12901 caggttgatg tgctggcgct ggtgcatcgt ttaagtcagg aacgtggtct gacggtcatt
    12961 gccgtgctgc acgatattaa tatggctgcg cgctactgtg attatctggt cgccctgcgc
    13021 ggtggtgaaa tgattgctca gggaacgcct gcggaaatta tgcgcggcga aaccctcgaa
    13081 atgatttatg gcatcccgat gggtattctg ccgcatccgg cgggtgctgc acctgtgagt
    13141 tttgtttatt gatgagcggc ttacctctta tttcgcgccg ccgactgtta acggcgatgg
    13201 cgctttctcc gttgttatgg cagatgaata ccgcccacgc ggcgattatt gatcccaatc
    13261 gtattgtggc gctggagtgg ttgccggtgg aattactgct ggcgctcggc atcgtgcctt
    13321 acggcgtggc ggacaccatc aactatcgcc tgtgggtcag cgaaccgcca ttgccggact
    13381 cagtgattga cgttggtttg cgtacagaac ccaaccttga actgctgacc gaaatgaaac
    13441 cgtcgtttat gatctggtcg gcaggatatg gcccttcacc agaaatgctg gcacgtattg
    13501 cgccggggcg cggatttaac ttcagcgacg gcaaacagcc gctggcgatg gcgcgtaaat
    13561 cactgacgga aatggcagat ttacttaacc tgcaaagcgc agcagaaacg catttagcgc
    13621 aatatgaaga ctttatccgc agcatgaaac cccgctttgt gaagcgtggt gcacgcccgt
    13681 tattgctgac gacgcttatc gatccgcgcc atatgttggt cttcggtcca aacagcttgt
    13741 tccaggaaat tcttgatgag tacggcatcc caaatgcctg gcaaggggaa accaacttct
    13801 ggggcagtac cgccgtcagt atcgatcgtc tggcggcgta taaagacgtt gatgtgctct
    13861 gttttgatca cgacaacagc aaagacatgg atgcgctaat ggcaaccccg ctgtggcagg
    13921 ccatgccgtt tgtccgtgca ggacgttttc agcgcgttcc ggcggtctgg ttctatggcg
    13981 cgacgctttc ggcaatgcac tttgtgcgta ttctggataa cgccatcgga ggtaaagcgt
    14041 gagtaaacga attgcgcttt ttccggcgtt attgctggcg ctgctggtga ttgtcgctac
    14101 ggcgctcacc tggatgaact tctcgcaggc gttgccgcgc agccagtggg cgcaggccgc
    14161 ctggtcgccg gatattgacg tcatcgagca gatgattttt cactacagct tgttgccgcg
    14221 tctggcgatt tcgctgctgg tgggcgcggg cctggggctg gtgggcgtgc tgtttcagca
    14281 agtgctgcgt aacccgctgg cggaaccgac gacgttgggc gttgcaacag gcgcgcaact
    14341 ggggattacc gtcactacgc tctgggcgat cccaggtgct atggcgagcc agtttgctgc
    14401 gctggcaggg gcttgcgttg ttggcttaat tgtctttggc gtcgcgtggg ggaaacggct
    14461 gtcgccggta acgctgatcc tcgcggggct ggtagtgagc ctttattgtg gggcaatcaa
    14521 tcagttactg gttatcttcc atcacgacca gctacaaagc atgttcctgt ggagcaccgg
    14581 aacgctgacg caaaccgact ggggcggcgt tgagcgttta tggccgcagc tgctgggcgg
    14641 cgtgatgctg acgttattgc tacttcgccc gttaaccctg atggggcttg atgatggcgt
    14701 ggcgcgcaat ctcggtctgg ccttgtcgct ggcgcgtctg gcggcgctgt cgctggcgat
    14761 agtcatcagt gcgctgctgg tgaatgcagt ggggattatc ggctttatcg ggttgttcgc
    14821 gccgctgctg gcaaaaatgc tgggggcgcg gcgtctgctg ccacggttaa tgctggcgtc
    14881 gctgattggt gcgttaattc tgtggctttc tgatcaaatc atcctctggc tgactcgcgt
    14941 gtggatggaa gtttccaccg gttcggtcac tgcgttgatc ggtgcgccgc tgctactgtg
    15001 gctgctgccg cgtttacgca gcattagcgc gccggatatg aaggtcaacg atcgtgtcgc
    15061 ggctgaacgc caacatgtgc tggcgtttgc cctcgcgggc ggcgtgctgc tgttgatggc
    15121 tgtggtagtg gcgctgtcgt ttggtcgtga tgcgcacggc tggacgtggg cgagcggggc
    15181 gttgctcgag gatttaatgc cctggcgctg gccgcgaatt atggcggcgc tgtttgcggg
    15241 cgtcatgctg gcggtggcgg gctgtattat tcagcggctg accggaaacc cgatggcaag
    15301 cccggaagtg ctggggatta gctccggcgc ggcgtttggc gtggtgttga tgctgtttct
    15361 ggtgccgggt aatgcctttg gctggctgtt acctgcaggc agtctcggcg cggcggtgac
    15421 gctgttgatc attatgatcg ccgccggacg cggtggattt tccccacacc gcatgttact
    15481 ggcggggatg gcgttaagta ccgcgttcac catgcttttg atgatgttgc aggcaagtgg
    15541 tgacccgcga atggcgcaag tgctgacctg gatctccggt tcgacctaca acgcgaccga
    15601 tgcgcaggtc tggcgcacgg gaattgtgat ggtaattttg ctggcgatta acccgctgtg
    15661 ccgccgctgg ctgaccattt taccgctggg cggtgatacc gcacgagccg taggaatggc
    15721 gctgacgccg acgcgaattg cgctgctgct attagcggct tgcctgacgg cgaccgcgac
    15781 gatgactatt ggaccgttga gttttgttgg tttaatggca ccgcatattg cgcggatgat
    15841 gggttttcga cggacgatgc cacacatcgt aatttcggcg ctggtgggtg gtttactgct
    15901 ggtgttcgct gactggtgtg ggcggatggt attgtttcca ttccagatcc cggcggggct
    15961 gctgtcaacc tttatcggcg cgccatattt tatctatttg ttgagaaagc agagccgtta
    16021 atagaatgcc tcgcccggtg ggcgaggcaa aatcatcaca acttcgcaaa cacccgacgt
    16081 gcagcatcga tggtgttatt gatatcttcc atgctgtgcg ccacggacat aaagcccgct
    16141 tcaaacgctg acggtgccag ataaacacct tcgtccagca tcatatggaa gaaacgctta
    16201 aagcgttcca cgtcacaggc catcacatcc tgatagcacg tcacggactc ggcgtcggta
    16261 aagaaaatac cgaacatgcc gccaacgtgg tttaccacca gcggaattcc ggcttcttct
    16321 gccgcttcca gcagaccttc tgccagacgt gttgtcagct catccagcgt ctcgtgaacg
    16381 cccggctgtg cgacttcatt cagacaggcg aaaccagctg ccatcgcaat cgggttaccg
    16441 gaaagcgtac ccgcctgata gaccggacca gtcggggcca gcgcatccat tacatcacga
    16501 cgaccaccga atgcgcctac cggcattcca ccgccgatga ttttgccgag gcaggttaaa
    16561 tctggcacta cgccgtaata atcctgtgcg ccagctagcg ctacgcggaa accggtcatc
    16621 acttcatcga tgatcagcaa cgcgccaaat tcgtcacaca gcgcacgcag acctggcagg
    16681 aactctggca gcggtggaac gcagttcata ttgcctgcca ccggctcgac gataatacag
    16741 gcaatctcat gcgggtactg ttcgaacgcg gcgcgtacag aggccagatc gttataagta
    16801 caggttaagg tatgtttggc gaaatctgcc ggaacacccg gcgagtttgg ctggcctaac
    16861 gtgagtgcgc cagaaccggc tttcaccagc aggcagtcag cgtgaccgtg gtaacaacct
    16921 tcaaatttaa tgattttgtc gcgaccggta aaaccacggg ccaggcggat tgcgctcatg
    16981 gtcgcttcag tgccggagtt caccatgcgc accatatcca tggtcgggac cagttcggtc
    17041 accagttgcg ccattttcac ttccatttcg gtcggtgcac caaagcttaa accacgctcg
    17101 gcggcttcaa tcacggcatt gcggatcgcc ggatggttat ggcccagcac catcggcccc
    17161 caggaaccga cataatcgat ataggctttg ccatcaacat cgtacagata agcgccgtcc
    17221 gctttttcga taaacagtgg agtgccgccc acgccagtaa aggcgcgaac aggggagttc
    17281 acaccgccag ggatcagctc gcgcgctgcg ctgtaaagat tttcagactt actcatggag
    17341 ggttcctgat tcgtagaaaa agtgaatggc tgctattcta tgttattcat aacaagttaa
    17401 atactcgtca aacatcaggc tgcttgtact ggtgcaatcc tgatttctta gagtataaaa
    17461 gttttgtgca tttgaaacat tacgctttgc aaaggatttt catggaacgt gcgagtaaaa
    17521 tgccgtcatc ttatttgtat gaccaataag tgatcattgg atgaaaactg atactccctc
    17581 tttagaaaca ccgcaggccg cgcgcctgcg acgcagacaa ctgattcgcc aacttcttga
    17641 gcgcgataaa accccgttag ccattttgtt tatggcggca gtcgtcggca cgcttgtcgg
    17701 gctggctgcg gttgcttttg acaaaggtgt cgcctggttg cagaaccaac gtatgggggc
    17761 actggtacat actgctgata attatccgct actgttaacc gtcgcttttc tctgttcggc
    17821 ggtgctggcg atgttcggct actttttggt gcgcaaatac gcgccggaag caggtggttc
    17881 ggggatcccg gaaattgaag gggcgctgga agatcaacgt cccgttcgct ggtggcgtgt
    17941 gttgccggtg aaattctttg gcgggctggg aacactcggc ggaggcatgg tgctgggccg
    18001 cgaagggcca accgtacaga tcggcggcaa tatcggtcgc atggtgcttg atgttttccg
    18061 cctgaaaggt gatgaagcgc gccatacgct gctggcaacc ggtgctgcgg cggggctggc
    18121 tgcggccttt aacgccccgc tggcgggtat tttgtttatt atcgaagaga tgcgtccgca
    18181 gtttcgctat acgttaattt cgattaaagc ggtatttatt ggcgtcatta tgtcgaccat
    18241 tatgtaccgg atttttaatc atgaagttgc gttgattgac gtcggtaaac tttctgacgc
    18301 gccgcttaat acgctgtggc tttatctgat cctcggtatt atttttggca ttttcggccc
    18361 tatttttaat aaatgggtgc tggggatgca ggatttgctg caccgtgtgc acggcggcaa
    18421 tattaccaaa tgggtactga tgggcggtgc aattggcggt ctgtgtggat tgctggggtt
    18481 tgtggcacca gcaacgtcgg gcggcggttt taacctgatt cctatcgcta ccgcggggaa
    18541 tttcagcatg ggaatgctgg tgtttatctt cgtcgcgcgg gtcattacca ccttactctg
    18601 cttctcttcc ggcgcgccgg gcggtatttt tgccccgatg ctggcgctgg gtactgtgct
    18661 gggaaccgct ttcggaatgg ttgccgttga gctgtttccg caatatcacc ttgaggcggg
    18721 gacatttgct attgccggaa tgggggcatt actggcggca tctattcgcg cgccgttaac
    18781 ggggatcatt ctggttctgg aaatgaccga taactaccag ctcattttgc caatgattat
    18841 taccggtctt ggcgcaacac tattagcgca atttaccggc gggaaaccgc tatactcggc
    18901 gattcttgcg cgcacgctgg cgaaacagga agctgagcaa ctggcgcgaa gcaaggccgc
    18961 atcagccagc gagaatactt gaacgaaata ccagggtatt agataatggc gattattatt
    19021 gggttagaat ttgcccaatt gccgatgtcg tttggagcaa aatatgagtg atgacgtagc
    19081 actgccgctg gagtttaccg acgcagcagc caacaaagtt aaaagcctga tcgctgacga
    19141 agataacccg aatctgaaat tacgcgtgta tattaccggt ggcggttgca gcggcttcca
    19201 gtatggtttc acctttgatg atcaggtgaa cgaaggcgat atgaccatcg aaaaacaggg
    19261 cgttggcctg gtggttgatc cgatgagcct gcaatatctg gtcggcggtt ccgttgacta
    19321 taccgaaggt ctggaaggtt ctcgtttcat cgtgaccaac ccgaacgcga aaagcacctg
    19381 cggttgcggt tcttccttta gcatctaatc tgttgttcat gtgccggata aactatccgg
    19441 cacatcttct ctcttcaacg cccattctca tccagcgcaa acgtcggtag cttaagatgc
    19501 caacgaatag ccgccagccg aatcaatagc gtcacgacca tgcccatcat actggctgtt
    19561 tccagtggta cggaaaatgt gtaataagcc gtagcgtgga caataccgcc gataatacag
    19621 gctgttgcgt agatttctgt acgtaaaatc atggggatct cgcgggcaag tacatcacga
    19681 atgatcccgc cgccaacgcc agtaatgacg cccatacaaa ccgcgattaa cggaccggct
    19741 tccgcattaa aggctttatt cacgccaatg ccgacaaaca ctgccagacc aacggcgtcc
    19801 agcaccggca acatccattt tggtaagcgt cttggctggc gcaccagcac gatggtcagc
    19861 atgctggtga ccattgcaac gaccagatcg gtgggatctt tcacccaaaa taccgggccg
    19921 tgatccagcg ccatgtcgcg aattgttccg ccgcctactg cggtaaccac gcccaatacc
    19981 agaacaccaa aagggtccat acgcaatttt ccggctaaca aaacgccaga gatagcaaat
    20041 accgctgtgc cgactatatc cagccaatag acgagcattg ttcaatcccc actgagcacc
    20101 tgaaaaggtc aggcattaat ctacctgtga aagcgcatta cagagctgtt gtgcagcgag
    20161 gataatacgt gggcttgcac gttcaaacca gtcactcgtg agaggaataa cgggaatttt
    20221 gagctgttca ccccagtatt gtttgatttt aggaatttgg tccggtccgc ctgtaatgac
    20281 aatcgcctgt ggcgcgcgtg ctaacacctg ttcgcggcta acttgcggcc agggaacccg
    20341 gctgtcttta aagatgtttt ctccgccaca aacttcgagt acctggtttt gaatcgactc
    20401 ttttccacta gtaaatggcg gattaatgcc gaattgcaga aaaacacgtt ttttaggttt
    20461 atcagcatat tgcgctttca attgcgcgta ctggtccagc agggattgcg cggcttgttc
    20521 ggccttatcc ggttgcggac tccaggggac cagttgacgt aacgcattgg caatttgttc
    20581 aatgcttgtc gcatcgaccc acatcacttt tattcccaga gaagccagct ggtcaacctg
    20641 ccgctcggca ttacctccac gccaggcaat caccagatcg ggtttcagcg ccacaatgcg
    20701 ttccagattc atcccctgcc aggtggaaac ctgctcaatt ttttgcgctt gtggaggata
    20761 gtcggaatag ctgctgaccc caaccggcgt gatcccggcg gcaaaggcaa gttcagtgtt
    20821 ggcgggagaa agcgtgatga cgcgcggcgc ggcgttgagc cacagcggcg cgagaaaaga
    20881 cagggcgacc agcgccctga acagtgactt agccatgtgc cagtttctgc accagtgact
    20941 caaccatcag gctggactgt ttagcggcaa cagccaggaa ctcatcgaag ctaagatgag
    21001 actgttgatc ggccacgtcg gaaatggcgc gtacgacaac aaacgggacg ttgaaattgt
    21061 ggcagacatg ggcgattgcc gtcgcttcca tctctacagc aatggcctgt gggaagttgt
    21121 ggcggatttt cgccagacca acagaaccat tgatgaaagc gtcgccgctg acaatcaggc
    21181 cacgtacagc gtttagattc agttcggcaa tgcaggcctc agcggcagcg atcagtttat
    21241 cgtcagcttt aaagcctgcc gggcagcctg gtaactggcc gtattcataa ccaaatgccg
    21301 tgacatccgc gtcgtgataa cgcgcttcgt ccgagacaac gatatcgccc actttcagtg
    21361 ttggtgccag gccaccggca gaaccggtgt taataatcac atctggcttg cagtgttcca
    21421 acagcaaagt ggcacccagc gccgcagcga ctttaccgat gcccgatttc agaagcgcaa
    21481 cctcggttcc attcagttgg ccggtataga tttcgcaacc gccgagactg atagtttgac
    21541 ggttttcgat tttgtcacgc agtagcgtaa cttcttcttc cattgcacca atgatgccga
    21601 ttttcataga tttactcgcg ataagcccga tttgaaggca tagtttacca tgcgcttacg
    21661 gggaagcgta tttctcacgc gggagaggac atggcacaga ttgatttccg aaaaaaaata
    21721 aactggcatc gtcgttaccg ttcaccgcag ggcgttaaaa ccgaacatga gatcctacgg
    21781 atcttcgaga gcgatcgcgg gcgtatcatc aactctccgg caattcgtcg tctgcaacaa
    21841 aaaacccagg tttttccact ggagcgcaat gccgccgtgc gcacgcgtct tacccactcg
    21901 atggaagtcc agcaggtggg gcgctacatc gccaaagaaa ttttaagccg tctgaaagag
    21961 cttaaattac tggaagcata cggcctggat gaactgaccg gcccctttga aagcattgtt
    22021 gagatgtcat gccttatgca cgatatcggc aatccgccgt ttggtcattt tggcgaagcg
    22081 gcgataaatg actggtttcg ccagcgtttg cacccggaag atgccgaaag ccagcctctg
    22141 actgacgatc gctgcagcgt ggcagcacta cgtttacggg acggggaaga accgcttaac
    22201 gagctgcggc gcaaaatacg tcaggactta tgtcattttg aggggaatgc acaaggcatt
    22261 cgtctggtgc atacattgat gcggatgaat ctcacctggg cacaggttgg cggtatttta
    22321 aaatatactc gtccggcgtg gtggcgtggt gaaacgcctg agacacatca ctatttaatg
    22381 aaaaagccgg gttattatct ttctgaggaa gcctatattg cccggttgcg taaagaactt
    22441 aatttggcgc tttacagtcg ttttccatta acgtggatta tggaagctgc cgacgacatc
    22501 tcctattgtg tggcagacct tgaagatgcg gtagagaaaa gaatatttac cgttgagcag
    22561 ctttatcatc atttgcacga agcgtggggc cagcatgaga aaggttcgct ctattcgctg
    22621 gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt taagccgcag tacggaagat
    22681 cagtttttta tgtatttacg ggtaaacacc ctaaataaac tggtacccta cgcggcacaa
    22741 cgatttattg ataatctgcc tgcgattttc gccggaacgt ttaatcatgc attattggaa
    22801 gatgccagcg aatgcagtga tcttctaaag ctatataaaa atgtcgctgt aaaacatgta
    22861 tttagccatc cagatgtcga gcagcttgaa ttgcagggct atcgggtcat tagcggatta
    22921 ttagagattt atcgcccttt attaagcctg tcgttatcag actttactga actggtagaa
    22981 aaagaacggg tgaaacgttt ccctattgaa tcgcgcttat tcaacaaact ctcgacgcgc
    23041 catcggctgg cctatgtcga ggctgtcagt aaattaccgt cagattctcc tgagtttccg
    23101 ctatgggaat attattaccg ttgccgcctg ctgcaggatt atatcagcgg tatgaccgac
    23161 ctctatgcgt gggatgaata ccgacgtctg atggccgtag aacaataacc aggcttttgt
    23221 aaagacgaac aataaatttt taccttttgc agaaacttta gttcggaact tcgcgctata
    23281 aaacgaatct gaagaacaca gcaattttgc gttatctgtt aatcgagact gaaatacatg
    23341 aaaaaaacca cattagcact gagtgcactg gctctgagtt taggtttggc gttatctccg
    23401 ctctctgcaa cggcggctga gacttcttca gcaacgacag cccagcagat gccaagcctt
    23461 gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca ttaacgtaga aggtagcaca
    23521 accgttaata cgccgcgtat gccgcgtaat ttccagcagt tcttcggtga tgattctccg
    23581 ttctgccagg aaggttctcc gttccagagc tctccgttct gccagggggg ccagggcggt
    23641 aatggtggcg gccagcaaca gaaattcatg gcgctgggtt ccggcgtcat cattgatgcc
    23701 gataaaggct atgtcgtcac caacaaccac gttgttgata acgcgacggt gattaaagtc
    23761 cagctgagcg atggccgtaa gttcgacgcg aagatggttg gcaaagatcc gcgctctgat
    23821 atcgcgctga tccagatcca gaacccgaaa aatctgaccg caattaagat ggcggattct
    23881 gatgcactgc gcgtgggtga ttacaccgta gcaattggta acccgtttgg tctgggcgag
    23941 acggtaactt ccgggattgt ctctgcgctg gggcgtagcg gcctgaatgc tgaaaactac
    24001 gaaaacttca tccagaccga tgcagcgatc aaccgtggta actccggtgg tgcgttggtt
    24061 aacctgaacg gcgaactgat cggtatcaac accgcgatcc tcgcaccgga cggcggcaac
    24121 atcggtatcg gttttgctat cccgagtaac atggtgaaaa acctgacctc gcagatggtg
    24181 gaatacggcc aggtgaaacg cggtgagctg ggtattatgg ggactgagct gaactccgaa
    24241 ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt tcgtaagcca ggttctgcct
    24301 aactcctccg ctgcaaaagc gggcattaaa gcgggtgatg tgatcacctc actgaacggt
    24361 aagccaatca gcagctttgc cgccctgcgt gctcaggtgg gtactatgcc ggtgggcagc
    24421 aaactgaccc tgggcttact gcgcgacggt aagcaggtta acgtgaacct ggaactgcag
    24481 cagagcagcc agaatcaggt tgattccagc tccatcttca acggcattga aggcgctgag
    24541 atgagcaaca aaggcaaaga tcagggcgtg gtagtgaaca acgtgaaaac gggcactccg
    24601 gctgcgcaga tcggcctgaa aaaaggtgat gtgattattg gcgcgaacca gcaggcagtg
    24661 aaaaacatcg ctgaactgcg taaagttctc gacagcaaac cgtctgtgct ggcactgaac
    24721 attcagcgcg gcgacagcac catctacctg ttaatgcagt aatctccctc aaccccttcc
    24781 tgaatacggg aaggggttct ccttacaatc tgtgaacttc gccacaactc catacatctt
    24841 catcatcctt tgggcatttg cacaatgccg tacgttacgt acttccttat gctaagccgt
    24901 gcataacgga ggacttatgg ctggctggca tcttgatacc aaaatggcgc aggatatcgt
    24961 agcacgtacc atgcgcatca tcgataccaa tatcaacgta atggatgccc gcgggcgaat
    25021 tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac gaaggagcat tgctggtact
    25081 ttcacaggga cgagtcgtcg atatcgatga cgcggtggca cgtcatctgc acggtgtgcg
    25141 gcaggggatt aatctaccgt tacggctgga aggtgaaatt gtcggcgtaa ttggcctgac
    25201 aggtgaacca gagaatctgc gtaaatatgg cgaactggtc tgcatgacgg ctgaaatgat
    25261 gctggaacag tcgcggttga tgcacctgtt ggcgcaggat agccgtttgc gggaagaact
    25321 ggtgatgaac ctcattcagg ctgaggagaa tactcccgca cttactgaat gggcgcaacg
    25381 gctggggatc gatctcaatc aaccgcgagt ggtggctatt gttgaggtcg acagcggtca
    25441 gcttggcgtg gacagcgcaa tggcggagtt acagcaactg caaaatgcgc tgactacgcc
    25501 cgagcgtaat aatctggtgg cgattgtctc gctaaccgaa atggtggtgt tgaaaccggc
    25561 gttgaactct tttgggcgct gggacgcaga agatcatcgt aagagagttg aacaactgat
    25621 tacccgcatg aaagagtatg gccagttgcg ttttcgcgtt tcgctgggca actattttac
    25681 cggtcctggc agtattgccc gatcctatcg tacggcgaaa acgacgatgg tagtggggaa
    25741 acagcggatg ccagaaagtc gctgctattt ttatcaggat ctgatgttac ctgtgttact
    25801 cgacagtttg cgtggcgact ggcaggccaa cgaactggcg cgaccgctgg cgcggctgaa
    25861 agcgatggat aataacggct tgctgcgacg tacgctggcg gcgtggtttc gccataacgt
    25921 gcagccgctg gcaacgtcaa aggcgctgtt tattcatcgt aataccctgg agtatcggct
    25981 taatcgtata tcggaactca caggcttaga tctggggaat tttgatgaca ggttattgtt
    26041 gtatgtggcg ttacaactgg atgaacagcg atagggtaaa aatagtaaat gccgggaaat
    26101 atgctcgtct taattcaagt tgcatgtacg gcgactcgaa ttattcagcg tatactttac
    26161 ccggctggca gaatggcaat atgcaaaatt atttacgtgt cagcttttct aaatctgctt
    26221 ctatctcgct gattttattg gtcaccactg actccaggtg acgcaagtcg tcgagaattt
    26281 tacgcttaag gtcaacttcg ctacgatcac gctggcagat ttgatcaagc tcatcaatga
    26341 tataacgaag attagggctg atttcctgaa cttctttata tccctggcca ataccatcgg
    26401 cgaccaccgt tttgcgctga cgcggatatt taaacttcac actcttggcg aaaaactcgc
    26461 ctttatcttt ctgaaaatag attttcaaaa tatcgttatt ggcttcctgc cggaggctgt
    26521 aacgatcaat ttcctcagga ttggtaatgc ccaaactttt cagattgtcg tacatagttg
    26581 tacctcaaaa gagtcagtaa ttgtgcttat cttagcattt cgcctggcgg tttagctcga
    26641 cggagatcgc aggaagcctt ttcttccaga cttttatcat ggcctaaaaa tcaaaaaatt
    26701 acctgcttta ttctgataag agaattcgct ttctacacca gttccaggat tgtcacttcc
    26761 gggcggcaat tcagacgcaa gccatacaaa cttccgacac cccgagtcgt gtaaatttgt
    26821 cgctcaccaa aggcatttaa accggcggcg tagcgtttat cttctatggg cgcaaaaggc
    26881 tcaccaacca acggtatgcg taactgcccg ccgtgggtgt gaccacagag catcagatcc
    26941 cacttttcat tacgcatgac ttctttgctg tcgggattat gcgccagcac cagtcgtggc
    27001 agatttgctt cgctggcggg aggcggtttg cattgtccgg tccataaatc acccgtgcca
    27061 accagttcaa attgctgctt ttgagtggtg attagcatgg cttcgttaaa taacaccgtg
    27121 atccccgccg attttaatgc ctcgccaatt aagcgatttt tctctgtacc aacagggcga
    27181 tcgtggttgc cgtaacaggc aaatgtcggc gcacattcgg caagggagga gagtacgtca
    27241 ctaaacgccg aaaaattcag ctgcatatca aataatacgt aatcgccgcc cagcaatatc
    27301 aaatcgggct tttgtgctac gccaagagca attgcgtcag aaatcaggct taaaggaaca
    27361 aaacgagagt aatggagatc tgccagaaaa aggattttga aaggagcagc gttgtcttta
    27421 aaaaaggcga ggcggtgacg gattaattca agccagccag gttcccaata atgcatatag
    27481 ctgaggcttg atccggcagc taacgtcgca gcggctgcct gtaaaaaacg cctgcgtgtg
    27541 atcatcatcc atccctgcaa aaaatcgggc agcgtcatgc tgcccgtgta ctgattttta
    27601 gtcgatggta cgcagcagtt cgttaatgcc gactttgccg cgagttttcg cgtcaacttt
    27661 cttaacgata actgcacaat aaaggctgta tttgccatct ttcgacggca ggttgccaga
    27721 aacaaccacc gaaccagccg gaacgcgacc gtagtggatt tcgccggttt cacggtcgta
    27781 aatacgggtg ctctgaccaa tgtatacgcc catagagatg acggaacctt cttcgacaat
    27841 caccccttca accacttcag agcgtgcacc gatgaagcaa ttatcttcaa taatggtcgg
    27901 gttagcctgc agcggctcca gcacgccgcc gatgcctaca ccgccagaca gatggacgtt
    27961 tttaccaatt tgtgcacagg aaccgacagt ggcccaggta tcaaccatag tgccttcatc
    28021 aacatatgcg ccgatgttga cgtaagacgg catcagcacg gtgttacggg caataaatgc
    28081 accctgacgt accgccgctg gtggcacaac gcggaagcct tctttctgaa aacgcgcttc
    28141 gtcgtagtcg gcgaatttca tcggcacttt atcgaagtag cggctttctg ccccttcgat
    28201 cacctgatta tcattaatac ggaaagagag cagcaccgct tttttcaacc actgatgcgt
    28261 cacccactga ccgtcaattt tttccgctac acgcagtgcg ccggaatcca gcagggcgat
    28321 cacctgattt accgcttcgc gggttacggt gtctgcattg gctggcgtga tctcggcacg
    28381 gcgttcaaaa gcggtttcaa taatgttctg taactgctgc attgttaaac tcttttcata
    28441 tcagtaaaca catcaccctt tatcgtttgg attgagggcc tctgtcaacc gctgatgcac
    28501 ttcctgctgc aactcgttat taagcgcacg ccggtcagcg gtggcaatta tgaataaatc
    28561 ttctactcgc tcgccaatgg ttgtaattcg ggcgccatga agcgaaattc ccagatcggc
    28621 aaaaattttc ccgactcgcg ccagcagtcc aggttggtcg agggcgatca gttcgaggaa
    28681 cgatttgcgg tcggtatggg tcggcaaaaa cgtcacttcg gtttcaacag taaaatggcg
    28741 caatttggcg ggttggcgac ggggctgcgg tggctgccag ctactttgcg tcagcacctg
    28801 ctccagacca aaccgaataa cctcatgacg atctgcggat agcgggctac catcgggttc
    28861 cagcacgatg aaggtatcca tcgccatacc gtcgcgagtg gtgaaaattt gtgcgtcgtg
    28921 aacacttaaa ttgcggcggt ctaattcggc acagacggcg gcaaacagat aggggcggtc
    28981 cgggctccag ataaaaatct cggtgcctcc acgcgtagcc tgcggactaa gcaataccag
    29041 cggtttgctt aaatcatgct gtaataaatg gcgcgcatgc caggccagtt gatttgggct
    29101 atggcggaca aaatagttag cacgacagcg tgaccaaatt tggtgcagcg cctcttcgtc
    29161 gatgttatcc atgcgcagta gtgccagcgc ctggagttgg tgatggcgaa cccgttcgcg
    29221 catatccggc gtgttttgca ttccgcgtcg tagctgcttt tcggtggcga aatagagttc
    29281 gcgcaacaga ctttgcttcc agctattcca cagcgtttcg ttggtggcgc atatatcggc
    29341 cactgtcagg cataccagat aacgaagtcg gttttctgtt tgcacttctt cggcaaactg
    29401 cttgatcact tccgggtcct gaatatcgcg gcgttgggcg gtcaccgaca tcagcaagtg
    29461 ctggcgaacc agccacgcca ccagctgtgt ttcgcgcgag ttaagcccgt ggagttcggc
    29521 aaaatgcacc acgtcttgtg caccgagaat ggagtggtcg ccgccacgcc ctttggcgat
    29581 atcgtgaaac agcgcggcga tgaaaatcag ctcagttgac ggcaggcgcg gccagacgtc
    29641 tacacacaac ggatggcgct ggcgcgtttc ttcactggca aaactctcca gtttcagcat
    29701 cacgcgaata gtatgttcat ccaccgtgta ggcgtggaac aggtcaaact gcatctgccc
    29761 gacgatatgc gaccattggg gcatatacgc gccgagcacg ctatgacgat gcatcggtaa
    29821 tagtccgcga cgcaccgcac cagggtgacg cagaatgctc aaaaacagtt ttcgcgcttc
    29881 cggaatatta cacagcggtt gttgcaaatg gcgacgggcg tggcgtaact ggcgcagcgt
    29941 ggtagagtaa atgccggtga tcgcactgtt gcgcaccatg gtgtagaaca tacgcaagat
    30001 ggcttccggc tggcgcataa atagtgtttc atcacgcagg tcgatcagcg ttccgcgtag
    30061 ctgaaactca tcgtcgattg gacgtggttt ttcgtcggcg ggaagggcga ggatggcttc
    30121 atcgaacagt tgcagcagca tctggttgag ttcactgacg cggcgtgtaa cgcggaagta
    30181 atccttcatc atccgctcga ccggttcgtt accttcgcca ctgtaattca gacgctgggc
    30241 gacgctaagc tggcgatcaa acagcaggcg attatcgtaa cggctgacga ccagatgcag
    30301 ggcaaagcga atacgccaca atatatgcag acattcgttt aattccgctc gctccgctga
    30361 ggttaagaag ccaaacccga ccatttcatc cagcgatgtt gcgccaaaat gacggcgggc
    30421 cacccattgc agagtgtgaa tatcgcgcaa gccgccagga ctacttttga tgtctggttc
    30481 aaggttgtag ctggtgccgt ggtaacgctg atggcgctgg ttctgttctt caactttcgc
    30541 tgcgtagaac ttgtcggaag gccagaatcc ttcgctaaaa atatgttttt gcagttcgag
    30601 gaagagcgct acatcgccaa ttaataagcg ggattcgatt aaattggtgg cgacggttaa
    30661 atccgataac ccttccagca tgcactcttc aagcgtgcgc acgctatgac cgacttccag
    30721 ctttacatcc cagagcagcg ttaacagctc gcccactttt tgcgcctgat catccgggag
    30781 ctttttacgg cttaaaatca gtaaatcgat gtcggaaagt ggatgcagct cgccacgacc
    30841 ataaccaccg acggcaacca atgccaggtc ggcaatctgg ctgaatcccg cttcaatcca
    30901 taatcgttgc aggagttggt cgataaactc ggtgcgtgcc tcaatcaact gttctgcgga
    30961 gatcccattg tcaaaggcat cacccagcca acgctggaaa gtatcgatat gggcttttat
    31021 cccacagacg gttaattcat cacggggcca ggcgcatgga ttttgcggtt gaccgggcag
    31081 ggtggggaga gcggtgtttg cgtactgttc tggaagggta ttcattgtgc gccacccata
    31141 agattaaatt atcgcattaa aaaagccggc attcgccggc ttcatcttat tcgtcgtgcg
    31201 agattatcgc cgggatggtg tcatccttgc gtagcgtcag aatttcgcag ccgttatcag
    31261 tcaccacaat agtatgctca tattgtgcag acaagctgcg atctttggtt tttaccgtcc
    31321 agccatcttt catggtgcgg atctcttttt tacccgcgtt gaccattggc tcgatggtga
    31381 acgtcatccc aggtttcagt acgacgttgg tttcacggga gtcatagtgc agcacctgcg
    31441 gttcttcgtg gaagccgcga ccaataccgt gtccacaata ttcacgaacg acggagaagc
    31501 cttctgcttc gacaaatttc tgaattgccg caccgatttc gcgcagatta atgccaggtt
    31561 ttaccatgcg tagcgccagg tacaggcttt cttgcgtgat gcggcacaga cgttcgccca
    31621 tgatggtcgg cttaccgacg ataaacattt tcgaggtatc gccgtggaaa ccatctttga
    31681 ttacggtgac atcaatgtta acgatatcgc catctttcag cagcttggcg tcgtccggga
    31741 taccgtggca caccacttca ttaatagaaa tgcaaacgga tttcggatag ccgtgatagc
    31801 cgaggcaggc agaaaccgcg tgttgttcat taacaatgta atcattacag atgcgatcca
    31861 gctcgccggt gctgacgccc ggtttaacat acggttcgat catctccagc acttcggcag
    31921 ccagtcggcc agcgacgcgc attttttcga tatcttctgg ggtcttgatt gagatagcca
    31981 ttaattctgt ccatcagcgt cggtgatacc gacaatatat atgtaagtgc cgtcaatggt
    32041 atcacacccg ggcaaattga gaatcattct gaatttcgcc aaacgtgcca ctgaaggttt
    32101 tctataatag aaaattcaac gtctgatgct gtacacagcg ccaacaatta ttggtgtcca
    32161 cgacgtattt gtggtataaa gcgcgccgga cttccgatcc atttcgtata cacagactgg
    32221 acggaagcga caatctcact ttgtgtaaca acacacacgt atcggcacat attccggggt
    32281 gccctttggg gtcggtaata tgggatacgt ggaggcataa ccccaacttt tatatagagg
    32341 ttttaatcat ggcaactgtt tccatgcgcg acatgctcaa ggctggtgtt cacttcggtc
    32401 accagacccg ttactggaac ccgaaaatga agccgttcat cttcggtgcg cgtaacaaag
    32461 ttcacatcat caaccttgag aaaactgtac cgatgttcaa cgaagctctg gctgaactga
    32521 acaagattgc ttctcgcaaa ggtaaaatcc ttttcgttgg tactaaacgc gctgcaagcg
    32581 aagcggtgaa agacgctgct ctgagctgcg accagttctt cgtgaaccat cgctggctgg
    32641 gcggtatgct gactaactgg aaaaccgttc gtcagtccat caaacgtctg aaagacctgg
    32701 aaactcagtc tcaggacggt actttcgaca agctgaccaa gaaagaagcg ctgatgcgca
    32761 ctcgtgagct ggagaaactg gaaaacagcc tgggcggtat caaagacatg ggcggtctgc
    32821 cggacgctct gtttgtaatc gatgctgacc acgaacacat tgctatcaaa gaagcaaaca
    32881 acctgggtat tccggtattt gctatcgttg ataccaactc tgatccggac ggtgttgact
    32941 tcgttatccc gggtaacgac gacgcaatcc gtgctgtgac cctgtacctg ggcgctgttg
    33001 ctgcaaccgt acgtgaaggc cgttctcagg atctggcttc ccaggcggaa gaaagcttcg
    33061 tagaagctga gtaataaggc ttgataactc ccccaaaata gttcgagttg cagaaaggcg
    33121 gcaagctcga gaattcccgg gagcttacat cagtaagtga ccgggatgag cgagcgaaga
    33181 taacgcatct gcggcgcgaa atatgaaggg ggagagccct tatagaccag gtagtacacg
    33241 tttggttagg gggcctgcat atggccccct ttttcacttt tatatctgtg cggtttaatg
    33301 ccgggcagat cacatctccg aggattttag aatggctgaa attaccgcat ccctggtaaa
    33361 agagctgcgt gagcgtactg gcgcaggcat gatggattgc aaaaaagcac tgactgaagc
    33421 taacggcgac atcgagctgg caatcgaaaa catgcgtaag tccggtgcta ttaaagcagc
    33481 gaaaaaagca ggcaacgttg ctgctgacgg cgtgatcaaa accaaaatcg acggcaacta
    33541 cggcatcatt ctggaagtta actgccagac tgacttcgtt gcaaaagacg ctggtttcca
    33601 ggcgttcgca gacaaagttc tggacgcagc tgttgctggc aaaatcactg acgttgaagt
    33661 actgaaagca cagttcgaag aagaacgtgt tgcgctggta gcgaaaattg gtgaaaacat
    33721 caacattcgc cgcgttgctg cgctggaagg cgacgttctg ggttcttatc agcacggtgc
    33781 gcgtatcggt gttctggttg ctgctaaagg cgctgacgaa gagctggtta aacacatcgc
    33841 tatgcacgtt gctgcaagca agccagaatt catcaaacca gaagacgtat ccgctgaagt
    33901 ggtagaaaaa gaataccagg tacagctgga tatcgcgatg cagtctggta agccgaaaga
    33961 aatcgcagag aaaatggttg aaggccgcat gaagaaattc accggcgaag tttctctgac
    34021 cggtcagccg ttcgttatgg aaccaagcaa aactgttggt cagctgctga aagagcataa
    34081 cgctgaagtg actggcttca tccgcttcga agtgggtgaa ggcatcgaga aagttgagac
    34141 tgactttgca gcagaagttg ctgcgatgtc caagcagtct taattatcga aaaggagccg
    34201 cctgagggcg gcttcttttt gtgcccatct tgtaaattca gctaaccctt gtggggctgc
    34261 gctgaaaagc gacgtacaat gtcgctagta ttaattcatt tcaatcgttg acagtctcag
    34321 gaaagaaaca tggctaccaa tgcaaaaccc gtctataaac gcattctgct taagttgagt
    34381 ggcgaagctc tgcagggcac tgaaggcttc ggtattgatg caagcatact ggatcgcatg
    34441 gctcaggaaa tcaaagaact ggttgaactg ggtattcagg ttggtgtggt gattggtggg
    34501 ggtaacctgt tccgtggcgc tggtctggcg aaagcgggta tgaaccgcgt tgtgggcgac
    34561 cacatgggga tgctggcgac cgtaatgaac ggcctggcaa tgcgtgatgc actgcaccgc
    34621 gcctatgtga acgctcgtct gatgtccgct attccattga atggcgtgtg cgacagctac
    34681 agctgggcag aagctatcag cctgttgcgc aacaaccgtg tggtgatcct ctccgccggt
    34741 acaggtaacc cgttctttac caccgactca gcagcttgcc tgcgtggtat cgaaattgaa
    34801 gccgatgtgg tgctgaaagc aaccaaagtt gacggcgtgt ttactgctga tccggcgaaa
    34861 gatccaaccg caaccatgta cgagcaactg acttacagcg aagtgctgga aaaagagctg
    34921 aaagtcatgg acctggcggc cttcacgctt gctcgtgacc ataaattacc gattcgtgtt
    34981 ttcaacatga acaaaccggg tgcgctgcgc cgtgtggtaa tgggtgaaaa agaagggact
    35041 ttaatcacgg aataattccc gtgatggata aataagggta agattccgcg taagtatcgc
    35101 gggggcgtaa gtctggttat aaggcgttat tgttgcaggc agtttggtca cggccagcgc
    35161 gcagcaaccg gagcgtacaa aagtacgtga ggatggcgag cactgcccgg ggccaaaatg
    35221 gcaaataaaa tagcctaata atccagacga ttacccgtaa tatgtttaat cagggctata
    35281 cttagcacac ttccactgtg tgtgactgtc tggtctgact gagacaagtt ttcaaggatt
    35341 cgtaacgtga ttagcgatat cagaaaagat gctgaagtac gcatggataa atgcgtagaa
    35401 gcgttcaaaa cccaaatcag caaaatacgc acgggtcgtg cttctcccag cctgctggat
    35461 ggcattgtcg tggaatatta cggcacgccg acgccgctgc gtcagctggc aagcgtaacg
    35521 gtagaagatt cccgtactct gaaaatcaac gtgtttgatc gttcaatgtc tccggccgtt
    35581 gaaaaagcga ttatggcgtc cgatcttggc ctgaacccga actctgcggg tagcgacatc
    35641 cgtgttccgc tgccgccgct gacggaagaa cgtcgtaaag atctgaccaa aatcgttcgt
    35701 ggtgaagcag aacaagcgcg tgttgcagta cgtaatgtgc gtcgtgacgc gaacgacaaa
    35761 gtgaaagcac tgttgaaaga taaagagatc agcgaagacg acgatcgccg ttctcaggac
    35821 gatgtacaga aactgactga tgctgcaatc aagaaaattg aagcggcgct ggcagacaaa
    35881 gaagcagaac tgatgcagtt ctgatttctt gaacgacaaa aacgccgctc agtagatcct
    35941 tgcggatcgg ctggcggcgt tttgcttttt attctgtctc aactctggat gtttcatgaa
    36001 gcaactcacc attctgggct cgaccggctc gattggttgc agcacgctgg acgtggtgcg
    36061 ccataatccc gaacacttcc gcgtagttgc gctggtggca ggcaaaaatg tcactcgcat
    36121 ggtagaacag tgcctggaat tctctccccg ctatgctgta atggatgatg aagcgagtgc
    36181 gaaacttctt aaaacgatgc tacagcaaca gggtagccgc accgaagtct taagtgggca
    36241 acaagccgct tgcgatatgg cagcgcttga ggatgttgat caggtgatgg cagccattgt
    36301 tggcgctgct gggctgttac ctacgcttgc tgcgatccgc gcgggtaaaa ccattttgct
    36361 ggccaataaa gaatcactgg ttacctgcgg acgtctgttt atggacgccg taaagcagag
    36421 caaagcgcaa ttgttaccgg tcgatagcga acataacgcc atttttcaga gtttaccgca
    36481 acctatccag cataatctgg gatacgctga ccttgagcaa aatggcgtgg tgtccatttt
    36541 acttaccggg tctggtggcc ctttccgtga gacgccattg cgcgatttgg caacaatgac
    36601 gcccgatcaa gcctgccgtc atccgaactg gtcgatggga cgtaaaattt ctgtcgattc
    36661 ggctaccatg atgaacaaag gtctggaata cattgaagcg cgttggctgt ttaacgccag
    36721 cgccagccag atggaagtgc tgattcaccc gcagtcagtg attcactcaa tggtgcgcta
    36781 tcaggacggc agtgttctgg cgcagctggg ggaaccggat atgcgtacgc caattgccca
    36841 caccatggca tggccgaatc gcgtgaactc tggcgtgaag ccgctcgatt tttgcaaact
    36901 aagtgcgttg acatttgccg caccggatta tgatcgttat ccatgcctga aactggcgat
    36961 ggaggcgttc gaacaaggcc aggcagcgac gacagcattg aatgccgcaa acgaaatcac
    37021 cgtagctgct tttcttgcgc aacaaatccg ctttacggat atcgccgcgt tgaatttatc
    37081 cgtactggaa aaaatggata tgcgcgaacc acaatgtgtg gacgatgtgt tatctgttga
    37141 tgcgaacgcg cgtgaagtcg ccagaaaaga ggtgatgcgt ctcgcaagct gaggataatc
    37201 cggctacaga gagtcgcgct atttgttagc gtagggcttc agtgatatag tctgcgccat
    37261 ctgatcgtaa gtagttggct ttataaggtc aggtatgccg tggttttaca cggctttttt
    37321 ttgtataggc ttcagtattc ctgagtaccg taaaccctgt cagggaataa gaaacgcgtg
    37381 atgttgtctg ctactcaacc acttagcgaa aaattgccag cgcatggctg tcgtcatgtt
    37441 gcgatcatta tggacggcaa tggtcgctgg gcaaaaaagc aagggaagat tcgtgccttt
    37501 gggcataaag ccggggcaaa atccgtccgc cgggctgtct cttttgcggc caacaacggt
    37561 attgaggcgt taacgctgta tgcctttagt agtgaaaact ggaaccgacc agcgcaggaa
    37621 gtcagtgcgt taatggaact gtttgtgtgg gcgctcgata gcgaagtaaa aagtctgcac
    37681 cgacataacg tgcgtctgcg tattattggc gataccagtc gctttaactc gcgtttgcaa
    37741 gaacgtattc gtaaatctga agcgctaaca gccgggaata ccggtctgac gctgaatatt
    37801 gcggcgaact acggtggacg ttgggatata gtccagggag tcaggcaact ggctgaaaag
    37861 gtgcagcaag gaaacctgca accagatcag atagatgaag agatgctaaa ccagcatgtc
    37921 tgtatgcatg aactggcccc tgtagattta gtaattagga ctggggggga gcatcgcatt
    37981 agtaactttt tgctttggca aattgcctat gccgaacttt actttacaga tgttctctgg
    38041 cccgatttcg atgaacaaga ctttgaaggg gcgttaaatg cctttgctaa tcgagagcgt
    38101 cgtttcggcg gcaccgagcc cggtgatgaa acagcctgat gggggtcgct tttgctgaag
    38161 tatcgcctga tatctgcttt tgtgttaata cccgttgtca tcgcggcgtt gtttctgttg
    38221 ccgccggtgg ggttcgccat tgtaacgctg gtggtctgca tgctggcagc gtgggaatgg
    38281 ggacagctta gcggttttac cactcgttcg cagcgagtat ggttggcggt gttatgcggg
    38341 ttattgttgg cgctgatgct ttttctgttg ccggaatatc accgaaatat tcatcaaccg
    38401 ctggttgaaa tctcactttg ggcttcgctg ggttggtgga ttgtcgcgct attgctggtg
    38461 ctgttttacc ctggttccgc agcaatctgg cgtaactcta aaacattgcg ccttattttt
    38521 ggcgtgctaa ccattgttcc cttcttctgg ggcatgctgg cgttacgggc ctggcactac
    38581 gacgagaatc attacagtgg cgcaatatgg ctgctctatg tcatgatcct ggtatggggc
    38641 gctgactctg gcgcatatat gtttggcaaa ttgtttggta aacataagct ggcaccgaag
    38701 gtttctccgg gtaaaacctg gcaaggcttt ataggtggac tcgcgaccgc agcggtaatc
    38761 tcatggggtt atggcatgtg ggcgaatctc gacgtcgctc ccgtcacctt actcatttgc
    38821 tctattgtcg cagcgttagc ctcagtgctc ggcgatctga ccgagagtat gtttaagcgt
    38881 gaagcaggaa ttaaggacag cggtcattta attccaggac acggtggtat tttagatcgt
    38941 attgatagcc tgacggctgc ggtaccggtc tttgcttgct tgttgttact ggtattcagg
    39001 acgctttaac ggaaggtaat atgctgagtt ttctctggga tttggcttcg ttcatcgttg
    39061 cactgggtgt acttatcacc gtgcatgaat ttggtcattt ctgggttgcc cggcgttgtg
    39121 gtgttcgcgt tgagcgtttc tcaatagggt ttggtaaggc gctctggcgg cgaactgata
    39181 agctcggcac cgaatatgtt atcgccctga tcccgctggg cggttatgtc aaaatgctgg
    39241 atgagcgcgc agaaccggtc gttccggaac tccgccacca tgccttcaat aataaatctg
    39301 tcggccaacg agcggcgatt attgccgcag gtccggttgc aaacttcatt tttgctatct
    39361 ttgcctactg gctggttttt attattggtg tgcctggcgt acgtccggtg gttggcgaaa
    39421 tagcagccaa ttcgatagct gcggaagcac aaattgcacc aggtacggaa ctaaaagccg
    39481 tagatggtat cgaaacgcct gattgggatg ccgtgcgttt gcagttggtc gataaaattg
    39541 gcgatgaaag caccaccatt acggtagcgc catttggcag cgaccaacgg cgggatgtaa
    39601 agctcgattt acgtcactgg gcgtttgagc ctgataaaga agatccggta acttcgctgg
    39661 ggattcgtcc tcgtgggccg caaattgaac ctgtactgga aaatgtgcag ccaaactcgg
    39721 cggcaagcaa ggcaggtttg caagcaggcg acaggatcgt taaagtcgat ggtcagccct
    39781 taacgcagtg ggtgaccttt gtgatgcttg tccgggataa cccgggtaaa tccttagcgt
    39841 tagaaatcga aaggcagggg agtcccttgt ctttgacatt aatcccggag agtaaaccgg
    39901 gtaatggtaa agcgattggt tttgtcggta ttgagccgaa agtcattcct ttgccagatg
    39961 agtataaagt tgtacgccag tatgggccgt tcaacgccat cgtcgaagcc acggacaaaa
    40021 cgtggcagct gatgaagctg acggtcagta tgctgggaaa attgatcacc ggtgatgtga
    40081 aactgaacaa cctcagtggg ccgatctcta tcgccaaggg ggctgggatg acagcggaac
    40141 tcggggttgt ttattacctg ccgtttcttg cgcttattag cgtgaactta gggataatta
    40201 acctgtttcc gttgcccgta cttgacgggg ggcatctgct gttccttgcg atcgaaaaga
    40261 tcaagggcgg accggtatcc gagcgggttc aagacttttg ttatcgcatt ggctcgattt
    40321 tgctggtgct gttaatgggg cttgcacttt tcaatgattt ctctcggtta tgagagttag
    40381 ttaggaagaa cgcataataa cgatggcgat gaaaaagttg ctcatagcgt cgctgctgtt
    40441 tagcagcgcc accgtatacg gtgctgaagg gttcgtagtg aaagatattc atttcgaagg
    40501 ccttcagcgt gtcgccgttg gtgcggccct cctcagtatg ccggtgcgca caggcgacac
    40561 ggttaatgat gaagatatca gtaataccat tcgcgctctg tttgctaccg gcaactttga
    40621 ggatgttcgc gtccttcgtg atggtgatac ccttctggtt caggtaaaag aacgtccgac
    40681 cattgccagc attactttct ccggtaacaa atcggtgaaa gatgacatgc tgaagcaaaa
    40741 cctcgaggct tctggcgtgc gtgtgggcga atccctcgat cgcaccacca ttgccgatat
    40801 cgagaaaggt ctggaagact tctactacag cgtcggtaaa tatagcgcca gcgttaaagc
    40861 tgtcgtgacc ccgctgccgc gcaaccgtgt tgacctaaaa ctggtgttcc aggaaggtgt
    40921 gtcagctgaa atccagcaaa ttaacattgt tggtaaccat gctttcacca ccgacgaact
    40981 gatctctcat ttccaactgc gtgacgaagt gccgtggtgg aacgtggtag gcgatcgtaa
    41041 ataccagaaa cagaaactgg cgggcgacct tgaaaccctg cgcagttact atctggatcg
    41101 cggttatgcc cgtttcaaca tcgactctac ccaggtcagt ctgacgcctg ataaaaaagg
    41161 tatttacgtc acggtgaaca tcaccgaagg cgatcagtac aagctttctg gcgttgaagt
    41221 gagcggcaac cttgccgggc actccgctga aattgagcag ctgactaaga tcgagccggg
    41281 cgagctgtat aacggcacca aagtgaccaa aatggaagat gacattaaaa agcttcttgg
    41341 tcgctatggt tatgcctatc cgcgcgtaca gtcgatgcct gaaattaacg atgccgacaa
    41401 aaccgttaaa ttacgtgtga acgttgatgc gggtaaccgt ttctacgtgc gtaagatccg
    41461 ttttgaaggt aacgatacct cgaaagatgc cgtcctgcgt cgcgaaatgc gtcagatgga
    41521 aggtgcatgg ctggggagcg atctggtcga tcagggtaag gagcgtctga atcgtctggg
    41581 cttctttgaa actgtcgata ccgataccca acgcgttccg ggtagcccgg accaggttga
    41641 tgtagtctac aaggtgaaag aacgtaacac cggtagcttc aacttcggga ttggttacgg
    41701 tactgaaagt ggcgtgagct tccaggctgg tgtgcaacag gataactggt taggtacggg
    41761 ttatgctgtt ggtatcaacg ggaccaaaaa cgattaccag acctatgctg aactgtcggt
    41821 aaccaacccg tacttcaccg tagatggtgt aagcctcggt ggtcgtctct tctataatga
    41881 cttccaggca gatgacgccg acctgtccga ctataccaac aagagttatg gtacagacgt
    41941 gacgttgggc ttcccgatca acgaatataa ctcgctgcgt gcaggtctgg gttatgtaca
    42001 taactccctg tccaacatgc agcctcaggt tgcgatgtgg cgttatctgt actctatggg
    42061 tgaacatccg agcacctctg atcaggataa cagcttcaaa acggacgact tcacgttcaa
    42121 ctatggttgg acctataaca agcttgaccg tggttacttc ccgacagatg gttcacgtgt
    42181 caacctgacc ggtaaagtga ccattccggg atcggataac gaatactaca aagtgacgtt
    42241 agacacggcg acttatgtgc cgattgatga cgatcacaaa tgggttgttc tgggtcgtac
    42301 ccgctggggt tatggtgatg gtttaggcgg caaagagatg ccgttctatg agaacttcta
    42361 tgccggtggt tccagcaccg tgcgtggctt ccagtccaat accattggtc cgaaagcagt
    42421 ttacttcccg catcaggcca gtaattatga tccggactat gattacgaat gtgcgactca
    42481 ggacggcgcg aaagatctgt gtaaatcgga tgatgctgta ggcggtaacg ccatggcggt
    42541 tgccagcctc gagttcatca ccccgacgcc gtttattagc gataagtatg ctaactcggt
    42601 tcgtacttcc ttcttctggg atatgggtac cgtttgggat acaaactggg attccagcca
    42661 atattctgga tatccggact atagtgatcc aagcaatatc cgtatgtctg cgggtatcgc
    42721 attacaatgg atgtccccat tggggccgtt ggtgttctcc tacgcccagc cgttcaaaaa
    42781 gtacgatgga gacaaggcag aacagttcca gtttaacatc ggtaaaacct ggtaagtgtt
    42841 ctccacaaag gaatgtagtg gtagtgtagc gatgacttta ggcgatcaat ataagatcgc
    42901 cgggccacgc aaagaactgc accctccggt gcaaatggga tggtaaggag tttattgtga
    42961 aaaagtggtt attagctgca ggtctcggtt tagcactggc aacttctgct caggcggctg
    43021 acaaaattgc aatcgtcaat atgggcagcc tgttccagca ggtagcgcag aaaaccggag
    43081 tttctaacac gctggaaaat gagttcaaag gccgtgccag cgaactgcag cgtatggaaa
    43141 ccgatctgca ggctaaaatg aaaaagctgc agtccatgaa agcgggcagc gatcgcacca
    43201 aactggaaaa agacgtgatg gcccagcgcc agacttttgc tcagaaagcg caggcttttg
    43261 agcaggatcg cgcacgtcgt tccaacgaag aacgcggcaa actggttact cgtatccaga
    43321 ctgctgtgaa atccgttgcc aacagccagg atatcgatct ggttgttgat gcaaacgccg
    43381 ttgcttacaa cagcagcgat gtaaaagaca tcactgccga cgtactgaaa caggttaaat
    43441 aagtaatgcc ttcaattcga ctggctgatt tagcgcagca gttggatgca gaactacacg
    43501 gtgatggcga tatcgtcatc accggcgttg cgtccatgca atctgcacaa acaggtcaca
    43561 tcacgttcat ggttaaccca aaataccgtg agcatttagg cttgtgccag gcgtccgcgg
    43621 ttgtcatgac ccaggacgat cttcctttcg cgaaaagtgc cgcgctggta gtgaagaatc
    43681 cctacctgac ttacgcgcgc atggcgcaaa ttttagatac cacgccgcag cccgcgcaga
    43741 acattgcacc cagtgcggtg attgacgcga cggcgaagct gggtaacaac gtatcgattg
    43801 gcgctaacgc ggtgattgag tccggcgttg aactgggcga taacgtgatt atcggtgccg
    43861 gttgcttcgt aggtaaaaac agcaaaatcg gtgcaggttc gcgtctctgg gcgaacgtaa
    43921 ccatttacca tgagatccag atcggtcaga attgcctgat ccagtccgga acagtggtag
    43981 gcgcagacgg ctttggttat gccaacgatc gtggtaactg ggtgaagatc ccacagattg
    44041 gtcgcgtaat tattggcgat cgcgtggaga tcggtgcctg cactaccatt gatcgtggcg
    44101 cgctggatga cactgttatt ggcaatggtg tgatcattga taaccagtgc cagattgcac
    44161 ataacgtcgt gattggcgac aatacggcag ttgccggtgg cgtcattatg gcgggcagcc
    44221 tgaaaattgg tcgttactgc atgatcggcg gagccagcgt aatcaacggg catatggaaa
    44281 tatgcgacaa agtgacggtt acgggcatgg gtatggtgat gcgtcccatc actgaaccag
    44341 gcgtctattc ctcaggcatt ccgctgcaac ccaacaaagt ctggcgcaaa accgctgcac
    44401 tggtgatgaa cattgatgac atgagcaagc gtctgaaatc gcttgagcgc aaggttaatc
    44461 aacaagacta acgttccatc ttttgttcgc caaactttac ggcctgtctc attcttacga
    44521 ttgcggcagg ccgtgttatt attgtcgttt cttatatttt gacaggaaga gtatcttgac
    44581 tactaacact catactctgc agattgaaga gattttagaa cttctgccgc accgtttccc
    44641 gttcttactg gtggatcgcg tgctggattt tgaagaaggt cgttttctgc gcgcagtaaa
    44701 aaatgtctct gtcaatgagc cattcttcca gggccatttc cctggaaaac cgattttccc
    44761 gggtgtgctg attctggaag caatggcaca ggcaacaggt attctggcgt ttaaaagcgt
    44821 aggaaaactg gaaccgggtg agctgtacta cttcgccggt attgacgaag cgcgctttaa
    44881 gcgcccggtc gtgcctggcg atcaaatgat catggaagtc actttcgaaa aaacgcgccg
    44941 cggcctgacc cgttttaaag gggttgctct ggtcgacggt aaagtagttt gcgaagcaac
    45001 gatgatgtgt gctcgtagcc gggaggcctg atacgtgatt gataaatccg cctttgtgca
    45061 tccaaccgcc attgtggaag agggcgcgtc gattggcgcg aacgctcaca ttggtccttt
    45121 ttgtatcgtt ggaccccatg tcgaaattgg tgagggtacc gtactgaaat ctcacgttgt
    45181 cgtgaatggt catactaaaa ttggccgcga caatgagatt tatcagttcg cctccatcgg
    45241 cgaagtcaac caggatctta aatatgctgg cgaaccgacc cgtgtggaaa tcggcgatcg
    45301 taaccgcatt cgcgaaagcg tcaccattca tcgtggcaca gtccagggcg gtggattgac
    45361 gaaggtgggc agcgacaact tactgatgat caacgcgcac attgcgcacg attgtacggt
    45421 aggtaaccgc tgtattctcg ccaacaacgc aacgctggcg ggtcacgtat cggttgacga
    45481 cttcgcgatc atcggcggca tgaccgcagt ccatcagttc tgcatcattg gtgcgcacgt
    45541 gatggttggc ggctgctccg gtgtggcgca ggacgtccct ccttatgtca ttgcgcaggg
    45601 taaccacgca acgccgttcg gtgtcaatat cgaagggctg aagcgccgcg gattcagccg
    45661 tgaggcgatt accgctatcc gcaatgcgta taagctgatt tatcgtagcg gtaaaacgct
    45721 cgatgaagtg aaaccggaaa ttgctgaact ggcggaaaca tatccggaag tgaaagcctt
    45781 taccgatttc tttgcacgct caacgcgcgg tctgattcgt taatgactga acagcgtcca
    45841 ttaacgattg ccctggtcgc cggagaaacc tccggcgata tcctgggggc cggtttaatc
    45901 cgcgctctga aagaacgtgt gcccaacgcc cgctttgttg gtgttgccgg gccacgaatg
    45961 caggctgaag gctgcgaagc ctggtacgaa atggaagaac tggcggtgat gggcattgtt
    46021 gaagtgctcg gtcgtctgcg tcgcttactg catattcgtg ccgatctgac aacgcgtttt
    46081 ggcgaactga agccagatgt ttttgtcggt attgatgcgc ctgacttcaa tattaccctt
    46141 gaaggtaacc ttaaaaagca gggtatcaaa accattcatt atgtcagtcc gtccgtctgg
    46201 gcgtggcgac agaaacgcgt tttcaaaata ggcagagcca ccgatctggt gctcgcattt
    46261 ctgcctttcg aaaaagcgtt ttatgacaaa tataacgtgc cgtgccgttt tatcggtcac
    46321 accatggctg atgccatgcc gttagatcca gataaaaacg ccgcgcgtga tgtgctgggg
    46381 atccctcacg atgcccactg tctggcattg ttgccgggaa gccgtggcgc ggaagtggag
    46441 atgcttagcg ccgatttcct gaaaacggcc cagcttttgc gccagacgta tcctgatctg
    46501 gagatcgtgg tgccgctggt aaatgccaaa cgccgtgagc aatttgaacg catcaaagct
    46561 gaagtcgcgc cagacctttc ggttcatatg ctggatggaa tgggccgtga ggcgatggtc
    46621 gccagcgatg cggcgctatt ggcgtcgggt acggctgcac tggagtgcat gctggcgaaa
    46681 tgcccgatgg tggtggggta tcgcatgaag cctttcacct tctggttggc gaagcggctg
    46741 gtgaaaaccg attatgtctc gctgccaaac ctgctggcgg gcagagagtt agttaaagaa
    46801 ttattgcagg aagagtgtga gccgcaaaaa ctggctgcgg cgctgttacc gctgttggcg
    46861 aacgggaaaa ccagccacgc gatgcacgat accttccgtg aactgcatca gcagatccgc
    46921 tgcaatgccg atgagcaggc ggcacaagcc gttctggagt tagcacaatg atcgaatttg
    46981 tttatccgca cacgcacctg gttgcgggtg tggatgaagt cggacgcggg ccgttagttg
    47041 gcgcggtcgt caccgctgcg gtgatcctcg acccggcgcg cccgattgcc gggctgaatg
    47101 attccaaaaa gctgagcgaa aaacgccgtc tggcgctctg cgaagagatc aaagagaaag
    47161 cgttgagctg gagtctgggc cgagcggaac ctcacgaaat cgacgaactg aatattttac
    47221 acgcgaccat gctggcgatg cagcgtgccg tcgctgggct gcatattgcg ccggaatatg
    47281 tgttgattga tggtaaccgc tgcccgaaat taccgatgcc tgcgatggct gtggtgaaag
    47341 gcgatagccg cgtaccggaa atcagtgccg cgtctatcct ggcgaaagtg acgcgtgacg
    47401 ccgaaatggc ggcgctggat attgttttcc cgcaatatgg ttttgcccaa cataaagggt
    47461 atccaaccgc ttttcatctg gaaaaactgg ctgaatatgg cgcgactgaa catcatcgtc
    47521 gcagctttgg gcctgtcaaa cgcgtactgg gacttgcgtc ctgattcttg tgtcgagatt
    47581 aagtaaaccg gaatctgaag atgtctgaac cacgtttcgt acacctgcgg gtgcacagcg
    47641 actactcgat gatcgatggt ctggccaaaa ccgcaccgct ggtaaaaaag gcggcggcgt
    47701 tgggtatgcc agcactggcg atcaccgatt tcaccaacct ttgcggtctg gtgaagttct
    47761 acggagcggg acatggcgca gggattaagc ctatcgtcgg ggcagatttt aatgtccagt
    47821 gcgacctgct gggtgatgag ttaacccacc tgacggtgct ggcggcgaac aataccggct
    47881 atcagaatct gacgttgctg atctcaaaag cgtatcagcg cgggtacggt gcagctgggc
    47941 cgatcatcga tcgcgactgg cttatcgaat taaacgaagg gttgatcctt ctttccggcg
    48001 ggcgcatggg cgacgtcgga cgcagtcttt tgcgtggtaa cagcgcgctg gtagatgagt
    48061 gtgtcgcgtt ttatgaagaa cacttcccgg atcgctattt tctcgagctg atccgcaccg
    48121 gcaggccgga cgaagaaagc tatctgcacg ctgcggtgga actggcggaa gcacgcggtt
    48181 tgcccgttgt ggcgaccaac gacgtgcgct ttatcgacag cagcgacttt gacgcacacg
    48241 aaatccgcgt cgcgatccac gacggcttta ccctcgacga tcctaaacgc ccgcgtaact
    48301 attcgccgca gcaatatatg cgtagcgaag aggagatgtg cgagcttttt gccgacattc
    48361 ccgaagccct tgccaacacc gttgagatcg ccaaacgttg taacgtaact gtgcgtcttg
    48421 gtgaatactt cctgccgcag ttcccgaccg gggacatgag caccgaagat tatctggtca
    48481 agcgtgcaaa agaggggctg gaagagcgtc tggccttttt attccctgac gaggaagaac
    48541 gtgttaagcg ccgcccggaa tatgacgaac gtctggagac tgaacttcag gttatcaacc
    48601 agatgggctt cccgggttac ttcctcatcg ttatggaatt tatccagtgg tcgaaagata
    48661 acggcgtacc agtagggcca ggccgtggct ccggtgcggg ttcactggtg gcctacgcgc
    48721 tgaaaatcac cgacctcgat ccgctggaat ttgacctgct gttcgaacgt ttccttaacc
    48781 cggaacgtgt ctccatgcct gacttcgacg ttgacttctg tatggagaaa cgcgaccagg
    48841 ttatcgagca cgtagcggac atgtacggtc gtgatgcggt atcgcagatc atcaccttcg
    48901 gtacgatggc ggcgaaagcg gtgatccgcg acgtaggtcg cgtgctgggg catccgtacg
    48961 gctttgtcga tcgtatctcg aaactgatcc cgcccgatcc ggggatgacg ctggcgaaag
    49021 cgtttgaagc cgagccgcag ctgccggaaa tctacgaagc ggatgaagaa gttaaggcgc
    49081 tgatcgacat ggcgcgcaaa ctggaagggg tcacccgtaa cgccggtaag cacgccggtg
    49141 gggtggttat cgcgccgacc aaaattaccg attttgcgcc gctttactgc gatgaagagg
    49201 gcaaacatcc ggtcacccag tttgataaaa gcgacgttga atacgccgga ctggtgaagt
    49261 tcgacttcct tggtttgcgt acgctcacca tcatcaactg ggcgctggag atgatcaaca
    49321 agcggcgggc gaagaatggc gagccgccgc tggatatcgc cgcgatcccg ctggatgata
    49381 agaaaagctt cgacatgctg caacgctcgg aaaccactgc ggtattccag cttgaatcgc
    49441 gcggcatgaa ggacctgatc aagcgtctgc aacctgactg cttcgaagat atgatcgcac
    49501 tggtggcact gttccgcccc ggtccgttgc aatcagggat ggtggataac tttatcgacc
    49561 gtaaacatgg tcgcgaagag atctcctatc cggacgtgca gtggcagcat gaaagcctga
    49621 aaccggtact ggagccaacc tacggcatca tcctgtatca ggaacaggtc atgcagattg
    49681 cccaggtgct ttctggttat accctcggtg gcgcagatat gctgcgtcgt gcgatgggta
    49741 agaaaaagcc ggaagagatg gctaagcagc gttctgtatt tgctgaaggt gcagaaaaga
    49801 acggaatcaa cgccgaactg gcgatgaaaa tcttcgacct ggtggagaaa ttcgcgggtt
    49861 acggatttaa caaatcgcac tctgcggcct atgctttggt gtcatatcaa acgttatggc
    49921 tgaaagcgca ctatccggca gagtttatgg cggcggtaat gaccgccgat atggacaata
    49981 ccgagaaggt ggtgggcctg gtggatgagt gctggcggat ggggctgaaa atcctgccac
    50041 cagatataaa ctccggtctt taccatttcc acgtcaacga cgacggcgaa atcgtgtatg
    50101 gtattggcgc gatcaaaggg gtaggtgaag gtccgattga ggccatcatc gaagcccgta
    50161 ataaaggcgg ctacttccgc gaactgtttg atctctgcgc ccgaaccgac accaaaaagt
    50221 taaaccggcg ggtgctggaa aaactgatca tgtccggggc gtttgaccgt cttgggccac
    50281 accgtgcggc gctgatgaac tcgctgggcg atgcgttaaa agcggcagat caacacgcga
    50341 aagcggaagc tatcggtcag gccgatatgt tcggcgtgct ggccgaagaa ccggaacaaa
    50401 ttgaacaatc ctacgccagc tgccaaccgt ggccggagca ggtggtatta gatggggaac
    50461 gtgaaacgtt aggtctgtac ctgacgggac accctatcaa ccagtatcta aaagagattg
    50521 agcgttatgt cggaggcgta aggctgaaag acatgcaccc gacagaacgt ggtaaagtca
    50581 tcacggctgc ggggctcgtt gttgccgcgc gggttatggt caccaagcgc ggcaatcgta
    50641 tcggtatctg cacgctggat gaccgttccg ggcggctgga agtgatgttg tttactgacg
    50701 ccctggataa ataccagcaa ttgctggaaa aagaccgcat acttatcgtc agcggacagg
    50761 tcagctttga tgacttcagc ggtgggctta aaatgaccgc tcgcgaagtg atggatattg
    50821 acgaagcccg ggaaaaatat gctcgcgggc ttgctatctc gctgacggac aggcaaattg
    50881 atgaccagct tttaaaccga ctccgtcagt ctctggaacc ccaccgctct gggacaattc
    50941 cagtacatct ctactatcag agggcggatg cacgcgcgcg gttgcgtttt ggcgcgacgt
    51001 ggcgtgtctc tccgagcgat cgtttattaa acgatctccg tggcctcatt ggttcggagc
    51061 aggtggaact ggagtttgac taatacagga atactatgag tctgaatttc cttgattttg
    51121 aacagccgat tgcagagctg gaagcgaaaa tcgattctct gactgcggtt agccgtcagg
    51181 atgagaaact ggatattaac atcgatgaag aagtgcatcg tctgcgtgaa aaaagcgtag
    51241 aactgacgcg taaaatcttc gccgatctcg gtgcatggca gattgcgcaa ctggcacgcc
    51301 atccacagcg tccttatacc ctggattacg ttcgcctggc atttgatgaa tttgacgaac
    51361 tggctggcga ccgcgcgtat gcagacgata aagctatcgt cggtggtatc gcccgtctcg
    51421 atggtcgtcc ggtgatgatc attggtcatc aaaaaggtcg tgaaaccaaa gaaaaaattc
    51481 gccgtaactt tggtatgcca gcgccagaag gttaccgtaa agcactgcgt ctgatgcaaa
    51541 tggctgaacg ctttaagatg cctatcatca cctttatcga caccccgggg gcttatcctg
    51601 gcgtgggcgc agaagagcgt ggtcagtctg aagccattgc acgcaacctg cgtgaaatgt
    51661 ctcgcctcgg cgtaccggta gtttgtacgg ttatcggtga aggtggttct ggcggtgcgc
    51721 tggcgattgg cgtgggcgat aaagtgaata tgctgcaata cagcacctat tccgttatct
    51781 cgccggaagg ttgtgcgtcc attctgtgga agagcgccga caaagcgccg ctggcggctg
    51841 aagcgatggg tatcatcgct ccgcgtctga aagagctgaa actgatcgac tccatcatcc
    51901 cggaaccgct gggcggcgct catcgtaacc cggaagcgat ggcggcatcg ttgaaagcgc
    51961 aactgctggc ggatctggcc gatctcgacg tgttaagcac tgaagattta aaaaatcgtc
    52021 gttatcagcg cctgatgagc tacggttacg cgtaattcgc aaaagttctg aaaaagggtc
    52081 acttcggtgg ccctttttta tcgccacggt ttgagcaggc tatgattaag gaaggatttt
    52141 ccaggaggaa cacatgaaca tcattgccat tatgggaccg catggcgtct tttataaaga
    52201 tgagcccatc aaagaactgg agtcggcgct ggtggcgcaa ggctttcaga ttatctggcc
    52261 acaaaacagc gttgatttgc tgaaatttat cgaacataac cctcgaattt gcggcgtgat
    52321 ttttgactgg gatgagtaca gtctcgattt atgtagcgat atcaatcagc ttaatgaata
    52381 tctcccgctt tatgccttca tcaacaccca ctcgacgatg gatgtcagcg tgcaggatat
    52441 gcggatggcg ctctggtttt ttgaatatgc gctggggcag gcggaagata tcgccattcg
    52501 tatgcgtcag tacaccgacg aatatcttga taacattaca ccgccgttca cgaaagcctt
    52561 gtttacctac gtcaaagagc ggaagtacac cttttgtacg ccgggtcata tgggcggcac
    52621 cgcatatcaa aaaagcccgg ttggctgtct gttttatgat tttttcggcg ggaatactct
    52681 taaggctgat gtctctattt cggttaccga gcttggttcg ttgctcgacc acaccgggcc
    52741 acacctggaa gcggaagagt acatcgcgcg gacttttggc gcggaacaga gttatatcgt
    52801 taccaacgga acatcgacat cgaacaaaat tgtgggtatg tacgccgcgc catccggcag
    52861 tacgctgtta attgaccgca attgtcataa atcgctggcg catctgttga tgatgaacga
    52921 tgtagtgcca gtctggctga aaccgacgcg taatgcgttg gggattcttg gtgggatccc
    52981 gcgccgtgaa tttactcgcg acagcatcga agagaaagtc gctgctacca cgcaagcaca
    53041 atggccggtt catgcggtga ttaccaactc cacctatgat ggcttgctct acaacaccga
    53101 ctggatcaaa cagacgctgg atgtcccgtc gattcacttc gattctgcct gggtgccgta
    53161 cacccatttt catccgatct accagggtaa aagtggtatg agcggcgagc gtgttgcggg
    53221 aaaagtgatc ttcgaaacgc aatcgaccca caaaatgctg gcggcgttat cgcaggcttc
    53281 gctgatccac attaaaggcg agtatgacga agaggcgttt aacgaagcct ttatgatgca
    53341 taccaccacc tcgcccagtt atcccattgt tgcttcggtt gagacggcag cggcgatgct
    53401 gcgtggtaat ccgggcaaac ggctgattaa ccgttcagta gaacgagcgc tgcattttcg
    53461 caaagaggtc cagcggctgc gggaagagtc tgacggctgg tttttcgata tctggcaacc
    53521 gccgcaggtg gatgaagccg aatgctggcc cgttgcgcca ggcgaacgat ggcacggctt
    53581 taacgatgcg gatgccgatc atatgtttct cgatccggtt aaagtcacta ttctgacacc
    53641 ggggatggac gagcagggca atatgagcga ggaggggatc ccggcggcgc tggtggcaaa
    53701 attcctcgac gaacgtggga tcgtagtaga gaaaaccggc ccgtataacc tgctgtttct
    53761 ttttagtatt ggcatcgata aaaccaaggc aatgggatta ttgcgtgggt tgacggaatt
    53821 caaacgctct tacgatctca acctgcggat caaaaatatg ctacccgatc tctatgctga
    53881 agatcccgat ttctaccgca atatgcgtat tcaggatctg gcgcaaggga tccataagct
    53941 gattcgtaaa cacgatcttt ccggtttgat gttgcgggca tttgatacct tgccggagat
    54001 gatcatgacg ccacatcagg catggcagag acagattaaa ggcgaagtgg aaaccattgc
    54061 gctggaacaa ctggtcggta gagtatcggc aaatatgatc ctgccttatc cacctggcgt
    54121 accgctgctg atgccaggag aaatgctgac caaagagagc cgcacggtac tcgattttct
    54181 actgatgctt tgttccgtag ggcaacatta ccccggtttt gaaacggata ttcacggcgc
    54241 gaaacaggac gaagacggcg tttaccgcgt acgagtccta aaaatggcgg gataacttgc
    54301 cagagcggct tccgggcgag taacgtgctg ttaacaaata aaggagacgt tatgctgggt
    54361 ttaaaacagg ttcaccatat tgcgattatt gcgacggatt atgcggtgag caaagcgttt
    54421 tactgcgata ttcttggttt cacgctgcaa agcgaagtct atcgcgaagc gcgcgactcg
    54481 tggaaagggg atttggcgct taatgggcaa tatgtgattg agcttttctc atttccgttc
    54541 ccgccggagc gccccagccg accggaagct tgcggcctgc gtcatctggc ttttagcgtt
    54601 gatgatatcg atgcggcagt ggcgcacctt gaaagccata acgtgaagtg tgaagccatc
    54661 cgtgtcgatc catacacgca aaaacgcttc acgttcttta acgatccgga cgggctgccg
    54721 ttggaactgt atgagcagta aggcttgtca tcgccgcatt tgcccggtaa cgtgccgggc
    54781 attgctactg taaaatcgca tcatcatgac actcacgctc aatagacaac ttctctcctc
    54841 acgccagatt ctggtggcct ttagcggcgg gcttgactcc accgttctgc tgcatcagtt
    54901 ggtgcagtgg cggatggaaa atccgggcgt cactctgcgc gctatccatg tgcatcacgg
    54961 tttaagtgct aatgccgatg cctgggttag gcattgcgag aacatctgcc aacagtggca
    55021 ggtgccgctg gtggtcgagc gcgtgcaact tgcgcaagaa ggactgggca ttgaggccca
    55081 ggcgcggcag gcacgttatc aggcatttgc ccgcaccttg ttacccggtg aagtgctggt
    55141 caccgcgcaa catctcgacg atcaatgtga aacctttctg ctggcgctaa aacgcggtag
    55201 cggccctgcc gggctttcgg ctatggggga agtttcggag tttgccggaa cacggcttat
    55261 ccgcccgttg ctcgcccgca cgcggggaga actggtgcag tgggcgcgtc agtatgattt
    55321 acgctggatt gaagacgaaa gtaatcagga tgatagctac gatcgtaact tcctgcgcct
    55381 gcgcgtggtg ccgttattgc agcagcgttg gccgcatttt gccgaagcaa cggcccgcag
    55441 cgctgcgctt tgcgctgaac aagagagcct gttggatgaa ctgctggcgg atgatatagc
    55501 acactgtcaa acgtcgcagg ggacgctgca gattgcgcca atgctggcga tgagtgatgc
    55561 ccgccgcgcg gcgattatcc gccgctggct ggcagggaag aatgcaccga tgccttcccg
    55621 cgacgcgttg gtgaggatct ggcaggaagt ggcgctggcg cgggaagatg cttccccctg
    55681 tttacgcctg ggcgcgtttg aaatccgccg ctatcagtcg caactgtggt ggattaaatc
    55741 cgtcactggg caaagcgaaa ccattgtgcc gtggcagacg tggctacaac cgctggaatt
    55801 accggcggga ctgggaagtg tacagcttac tgcgggaggc gatattcgcc ctccgcgtgc
    55861 agacgaagcg gtcagcgtgc gtttcaaagc gccaggattg ctgcatatta tcgggcgcaa
    55921 cggcggacgt aagctgaaga aaatctggca agagctgggt gtgccgccgt ggctacgtga
    55981 caccacgcca ctgctgtttt atggcgaaac gttgattgcg gcggcagggg tatttgtgac
    56041 gcaagaaggt gtggctgaag gtgagaatgg cgtcagtttt gtctggcaga aaacgcttag
    56101 ttaagtgaaa gccggataag acgcttcaaa cgtcgcatcc ggcgaaagtc aatcaggact
    56161 cgctcaccac caccgtaccg atttccgggt ggctaaagct ggtaatttta tccagacgca
    56221 gctcgcgggt tgtaccagcg gcctcgacga ccagatactc cacatttttg cgggagacta
    56281 aatcgctggc cttcgcctgc aatttttcgc catctttcag ctcaagtgtc agcattaaat
    56341 gatgctggca ggcgagctca agattatcgt aatcatcaca attgattggt tgatacgtat
    56401 cattcattga cataatcgct caccagtaag tttgccgcag cgtatgctgc tttttccctg
    56461 acagcctcag aaagggcgtc gtcggcagcc atttcattca gcactttcaa aacgcagccc
    56521 agcgcgtccg gaacgtatcc taagtctccg ctggcgattt ccgcgtaccg cttgcgtatt
    56581 aactcacaat atttttccac atgccctcct gtcagcactc tgacttaacc gtggatgcaa
    56641 gtctaagcct acgaagataa actctgtttc gcaaggtgac tataccacac tcatttctgc
    56701 aatatcagcg ccgcaactgc acgtattccg ttacaatggc ctcctgattc gaaaggagtt
    56761 ttcttatggc gcttaaagcg acaatttata aagcgacggt taatgtggcc gatctcgacc
    56821 gcaaccagtt tctcgatgcc tctctgacgc tggcgcgcca cccttcagaa acccaggagc
    56881 gcatgatgct gcgcctgctg gcgtggctaa aatatgccga tgaacgtctg caatttaccc
    56941 gtggtttgtg tgccgatgat gagccggaag cgtggctgcg taacgatcat ttgggtattg
    57001 atttgtggat tgagctgggg ctgccggatg agcgacggat taagaaagcc tgcacccagg
    57061 ccgcagaagt ggcgctgttt gcctataata gtcgggcggc gcaaatctgg tggcagcaaa
    57121 atcagagcaa atgcgcgcag ttttccaatc tttccatctg gtatctggac gatgaacaac
    57181 tggcgaaagt gagcgccttt gccgatcgta ccatgacgct gcaggcaacg attcaggatg
    57241 gcgtgatctg gttatcggat gataagaata atctggaagt gaacttaacc gcctggcaac
    57301 aaccttcatg attgtgattt cccgacatgt tgctatcccc gatggtgagc ttgagatcac
    57361 cgccattcgt gcgcagggcg cgggcgggca gcatgttaat aagacctcaa cggctattca
    57421 tctgcgtttt gacattcggg cgtccagcct gccagagtat tacaaagaac gtctgctcgc
    57481 cgccagccat cacttgatca gcagtgatgg cgtgattgtc attaaggcac aggaataccg
    57541 cagtcaggaa ctgaaccgcg aagcggctct ggcccggctg gtggcagtga ttaaagattt
    57601 aacaacagaa caaaaagccc gacgacccac gcgccccacc cgtgcatcga aagagcgcag
    57661 gctggcatcg aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tgcgcagcgg
    57721 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
    57781 tcagcctctt tactctgatg ggttgtaata atcgggccga agtcgatacg ctttctccgg
    57841 cgcaggctgc cgaactgaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgctg
    57901 attgcgaagg aatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
    57961 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
    58021 gaaccgctga caagctagta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
    58081 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc gattgaatcg cagttcaact
    58141 atacgctaga accggcacaa tccagtttac ctatgacgcc gatgaccctg cggggcatgt
    58201 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
    58261 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
    58321 cggtgttatt gtcagtagaa ggtcacttta cgcttgaggg taatccggat accggtgcgc
    58381 cgactaaagt attggcaccc gatacggcgg gtaaatttta tccgaaccag gattgcagta
    58441 gtttggggca gtaattgaaa gccggagcat tctccggctt ttatcactga gtcagcccaa
    58501 caaagcgacc cgccttgata taaatccctg gcttcatgtg cccaacatac tgtaactgcg
    58561 tttctggctt aaacgcagag atatcgccag tacgcatatg catcagatcg gcagggctaa
    58621 tccagcctga ttgcgccagc gtaagcggat gcccggcttt ggcaaatgca tcagtgacaa
    58681 attccgaaca aaaccacgac tttttgtctc cttcgcccac actgcttaac tgcgctttcg
    58741 ccaggccgct gacgcactgt tgacgaaaat cctcggagaa cggattcagt gagcacacct
    58801 ggcgagtcac cataaaggga ataaattcga caatgccgcg atagttataa ccgctatctt
    58861 tgattttatt agcaaacgcg gtgatatctg tggcttgttg cggggtaaga tccgggactc
    58921 gtaagacgaa aagcttatca ctgtgcttca tcgctttttt aagggaaacg atctggacgc
    58981 cagcgcctgt cgcttctgca acgttattat cacccagaaa gattgcaacg tgactcacag
    59041 aggaagtgct gaagacgcgg attccaaatg aggttacccc aaggcttgag gagaacagca
    59101 aatcgccggg tttgagatca ggtgccgtta tttcttttat tgattgttcg gtgaaagagc
    59161 tttgatgctg gaatttgact acccatgttt ttgcctcgac atctaccgct gtggcagagg
    59221 agtctggctg gctgatatca acggtacagg ccgaaaggaa aagaaagcag gggagaagca
    59281 ggcggcaatg cgcctttggt ttatccattt tatacaatcc atgtaaaaaa gggccctgaa
    59341 attcaggacc ctttctggca tcagccttta atctgtttca ccagatattc gacgatgtca
    59401 ccagtcttaa ttaactgttt ctcgccgtta cgacgatatt tatattcgat atcatcgttg
    59461 tcgaggttac ggtcgcccag cacaatagtg tgcggaatac cgatcagttc catatcagca
    59521 aacatcacgc ccggacgctc tttgcggtca tccagcagca cttcgatacc ttgtgcacgc
    59581 agttcgctgt acagtttctc agcaagctct tgtacgcgga aggatttgtg catgttcatc
    59641 ggcagaatcg ccacctggaa aggcgcgata gcgtcaggcc atacgatgcc gcgttcgtca
    59701 tagttctgct caattgccgc agccacaacg cgcgttaccc cgataccgta gcaacccatc
    59761 gtcaggattt gattacggcc atcttcaccc tgtacggagg ctttcagtgc ttcggagtac
    59821 ttggtaccca gctggaagat gtgaccaact tcgataccac gtttgatcag cagcgtaccc
    59881 tgaccatccg ggcttggatc gccagccacc acgttgcgga tatcagcaat ttccggggta
    59941 gcgacatcgc gatcccagtt gataccgaag tagtgtttac catcgatgtt agcaccagca
    60001 gcgaaatcac tcatcgccgc aacggtacgg tcaatcacca ccggaattgg catgtttacc
    60061 ggacccagtg agccagggcc ggctttaacc acggcacgaa tttcttcttc ggtcgcgaaa
    60121 gtcagcgggc ttgcaacctg cggcagtttt tctgctttaa cttcgttcag ctcgtggtca
    60181 ccgcgcacca gcagcgcaac cagcgggaag ctactgcctt caaccgcttt aaccagcagg
    60241 gtcttaaccg ttttctcaat cggcagattg aactgttcaa ccagttccgc gatggtcttc
    60301 gcgttcggcg tatcaaccag cgtcatttcc tgggtagcag cagcgcgcgg ttctttcggc
    60361 gcgatagctt ctgccagttc aatgttcgct gcatagtcag aggtgtcgga gaagaccaca
    60421 tcgtcttcac cgctttgcgc cagcacctgg aattcgtgag aggcgctacc gccgatagaa
    60481 ccggtatcgg cctgtacggc gcggaaatcc agccccatgc ggctgaagat tttgctgtag
    60541 gccgcataca ttgcatcgta ggtttcctgc agggattcct gagaggtatg gaaagagtaa
    60601 gcatctttca tcaggaattc gcgggaacgc atgacgccga aacgcgggcg cacttcatca
    60661 cggaacttag tctggatctg atagaagttc agcggcagct gtttgtaaga gctcagctcg
    60721 ttacgaatca ggtcggtaat cacttcttca tgagttgggc caagtacgaa cggacggtca
    60781 ccacggtcag caatacgcag caattccggg ccatactgtt cccaacgacc actctcttgc
    60841 cacagttcag atggctgaac caccggcatt aacacctcaa tcgcaccggc gttgttcatc
    60901 tcttcacgca cgatgttttc gacttttttc agaacgcgca cgccggtcgg cagccaggtg
    60961 tataacccgg aggccagctt gcggatcatc ccggcgcgca gcatcagctg atggctgatc
    61021 acctcggcgt cggcaggtgt ctccttgaga gtggagagca ggtattggct agtacgcatg
    61081 ttgttacggt tccagttgga aggtagaaca ggctcaaggt gagcctggga caaaaaaagt
    61141 gatttagttt accagtgcaa aagaaatgtc aaaagagaag ggcgtgaatt taacgcggtt
    61201 ccagcgcaaa gacttcaaaa cctgcgtcgg tgacgcgcca gcgaacgtta aaatcatgca
    61261 gccagacggc ataggttttg cccgtttcct cacctttacg ataggccggg cgcgggtcct
    61321 gcgctagtac ttcgcggata aacagcgtta actgcggata acgcttctcc agcgtcaaaa
    61381 gctgcttttc gacctctgcg gtaaaactca ccgccatctc tgcagctggc gcactttgcg
    61441 catagctggc actggcattt ggaagcgatt cggcaaaggg gagatacggt ttgatatcca
    61501 ctaccggcgt accatcgacc agatccagac tgccgagctt cagaatcacg ctgtctttat
    61561 ggcaaacaac ctctttcagc tctaccagcg acataccaat tgggttaggg cgaaaagtgg
    61621 agcgtgttgc gaaaaccccc attctggcat taccgccaag acgaggcgga cgcacagtcg
    61681 gacgccagcc gccttccatc gtttgatgaa agacgaaaag tatccataaa tggctgaacg
    61741 cttccaggcc gcgaacggcg tcggcttggt tgtagggagc aatgagatgc agttcgccgt
    61801 tggcgctttt taccagacct ggttggcgcg gaacggcgaa tttttcttta tagggcgagc
    61861 gaataacgcc tatttgctcg aactggaaac tgctcatttc gccgtaatgt taagcgcaga
    61921 accgatacat acagcctgac gatagcagcc tggcgtaccg ctggtgactt cgcagctatg
    61981 cagtaatacc gcattggctt tcattttcga ggcgttgatt tgcatccgct tacgcgcggt
    62041 tggaatgctc ggcggagagt cctgattaga ggcttggcaa gagtcgccac tgacttcacc
    62101 gagatcgcgg aacggtttgc cgactaattc ttctgcattg gtataaattc ggaccggcgt
    62161 ggcgcgcggc actttcggtt ttgcagactc cgctttcggc tggggtgcag tgctttgaac
    62221 gggttcgaca ggggatctgc ttaacatgga acagccgctt agcatgagtg ctactaaaca
    62281 gatcggtaaa gcacgcatag tatttcctca atgtatgatc aaaacgtcaa tattgaatca
    62341 ggagcttgta aaaatgacaa gacgggcaag cgcccgtcct gaatgatatt acaaattgtg
    62401 gaaacagcct aaaaattacc agcctttaac agcgccgccg ttaaacactt tgtttgctgc
    62461 ttcgtaaact tcgtcagact gataagcctg gacgaatttc ttcacgttct cggcgtcttt
    62521 gttatcttca cgcgttacga tcaggtttac gtacggggac tctttatctt cgacaaagat
    62581 accgtctttc gccggagtca ggccaatctg gctggcatag gtggtattga taactgccag
    62641 agcgatttgc gcgtcgtcca gagaacgcgg cagctgcggt gcttccagct caacaatttt
    62701 cagatttttg gggttctcaa caacatcaag aacggtcggc agcaggccaa cgccatcttt
    62761 cagtttgatc aagcccactt tttgcagcag cagcagtgaa cgaccaaggt tagttgggtc
    62821 gtttggcacg gcaacctgcg aaccatcctg cagttcatcc agtgatttga ttttcttgga
    62881 gtaacctgca atcggataaa caaaagtgtt gcctactgcg accagtttgt agccacgatc
    62941 tttcagttgc tgatcaaggt acggtttatg ctggaaggcg ttggcgtcga tatcgccttt
    63001 gctcaatgct tcgtttggca gaacatagtc gttgaatgtt accagctcaa cgtccaggcc
    63061 atatttgtct ttcgcaactt tctgcgcgac ttctgcaacc tgctgttcgg caccaacaat
    63121 cacgccgact ttaatgtggt ttggatcttt ttcatcctga ccgcagccta ccagtgccag
    63181 tgatccaatc agggctccca ctgccgcaaa ggttttgaat ttgaacgcca taccttattc
    63241 cttcttcaat tatttatgtt gtgttgaacg ttacttgcga gtgacagccc ggacgatgcg
    63301 gtcgcctgcg aactgaatta aataaaccag aatgaccagc aataccagta ccgtattcat
    63361 caccgtcgcg ttgtagccga tgtagccata ctgatagcca atctgaccta aaccaccggc
    63421 accgactgca ccacccatcg cggaataacc gaccagggta atcagggtga tagttgccgc
    63481 atttaccaga cccggcaacg cttccggtaa cagcacttta cggacgatct gcattggcgt
    63541 ggcacccatt gcgcgggaag cttcaattaa cccggttggg atctccagca gagcgttctc
    63601 gaccatacgg gcaataaacg gtgctgcacc aacggttaac ggaacaatcg ctgcctgcaa
    63661 accaatcgat gtaccaacaa taacgcgggt aaacggaatc atccatacca gcaagataat
    63721 gaacgggatg gaacggaaaa tgttcacaat cgcagaaacg gtacgataca gcttcgcgtt
    63781 agcaataatt tgccccggac gcgtgacata aagcaggacg ccaaccggca gaccaatcac
    63841 aaatccaaaa aaaccggata cgaaggtcat tgccagcgtt tcccatacgc cacgaaccag
    63901 cagccacatc atcggctcag acataaccca gtacctctac ttttacatgg tgttcctgca
    63961 gccaggtaat ggcggcttgc gtatcttgtt gtgtgccgtg catttcagtc agcatgatgc
    64021 cgaacttaac gccaccggcg taatccatct gcgcgctaat aatgttgttg ttgacgttga
    64081 aacgacgcgc ggtttcagaa agcagtgggg catcgaccga ttgaccggta aactccagac
    64141 gcaacatcgg cacgcagtca gtgaatggct ccgcttgcag acgttcctgg taatcttccg
    64201 ggatatccag atgcagggtc gactgaataa acttctgcgc cagcggcgtt ttcggatgcg
    64261 agaacacttc acttaccgtg tcctgctcga tcagttcgcc attgctgatg accgccacgc
    64321 aatcacaaat acgcttcaca acgtccattt cgtgggtgat caacagaatc gtcaaaccca
    64381 gacggcggtt gatgtctttc agcagttcga gaatagaacg tgtcgttgcc ggatccagcg
    64441 cgctggtggc ctcatcacac agcaatactt tgggattgct ggctaacgca cgggcaatcg
    64501 ccacacgctg tttctgccca ccggaaagat tcgacgggta gctatcatgc ttatcgccaa
    64561 gaccaaccaa tgacagcaat tccgtcacgc gacgtttgat ctcatctttc ggtgtgttgt
    64621 ccagctccag cggcagagcc acgttgccaa aaacagtacg cgaagagagc aggttaaaat
    64681 gctggaaaat cataccaatc tggcggcgag ctttggtcaa ctcggattct gacagcgtgg
    64741 tcagttcctg gccatcgacc agcacgctac cctcggttgg gcgctccagc aggtttacac
    64801 aacgtataag cgtactctta cccgcgccag aggcaccgat aacgccataa atttgtccag
    64861 ctggcacatg caggctgacg ttgttcaacg cctggatggt gcgggtgccc tggtggaaca
    64921 ctttggtgat attcgaaagt tttatcattg attatttatt atcgtcatta agttagtcgt
    64981 ggcatttcga atgcctggaa cgggcaacgc cttcaatgaa atggatgtta aggcatccag
    65041 acgtctaaat caatcaggtt tatgcgaaga gcactttctt gctggtcgaa acatgcgata
    65101 ctagcgtcac atgccttatt aaggagctat aaaaggtggc gaagagcgta cccgcaattt
    65161 ttcttgaccg tgatggcacc attaatgtcg atcacggcta tgtccatgag atcgacaact
    65221 ttgaatttat cgacggtgtt attgacgcca tgcgcgagct aaaaaaaatg ggctttgcgc
    65281 tggtggtagt aaccaaccag tctggcattg ctcgcggtaa atttactgaa gcacagtttg
    65341 aaacgctgac cgagtggatg gactggtcgc tggcggaccg cgatgtcgat ctggatggta
    65401 tctattattg cccgcatcat ccgcagggta gtgttgaaga gtttcgccag gtctgcgact
    65461 gccgcaaacc acatccgggg atgcttttgt cagcacgcga ttatttgcat attgatatgg
    65521 ccgcttctta tatggtgggc gataaattag aagatatgca ggcagcagct gcggcgaacg
    65581 tgggaacaaa agtgctggtg cgtacgggta aacctgttac gcctgaagca gaaaacgcgg
    65641 cggattgggt gttaaatagc ctggcagacc tgccgcaagc gataaaaaag cagcaaaaac
    65701 cggcgtaatg attaaaagat gagcggttga aataaaaatg catttttccg cttgtcttcc
    65761 tgagccgact ccctataatg cgcctccatc gacacggcgg atgtgaatca cttcacacaa
    65821 acagccggtt cggttaaaga gaaaaatcct gaaattc
//
LOCUS       DAZGHF010000009        64130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGHF010000009 DAZGHF010000000
VERSION     DAZGHF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.009"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(475..1323)
                     /locus_tag="ACHK3A_000657"
     CDS             complement(475..1323)
                     /locus_tag="ACHK3A_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO8738791.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            1532..2167
                     /gene="yfhb"
                     /locus_tag="ACHK3A_000658"
     CDS             1532..2167
                     /gene="yfhb"
                     /locus_tag="ACHK3A_000658"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO8738792.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2225..2728
                     /gene="tadA"
                     /locus_tag="ACHK3A_000659"
     CDS             2225..2728
                     /gene="tadA"
                     /locus_tag="ACHK3A_000659"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO8738793.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2725..4281)
                     /gene="mltF"
                     /locus_tag="ACHK3A_000660"
     CDS             complement(2725..4281)
                     /gene="mltF"
                     /locus_tag="ACHK3A_000660"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO8738794.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            4539..8426
                     /gene="purL"
                     /locus_tag="ACHK3A_000661"
                     /gene_synonym="purI"
     CDS             4539..8426
                     /gene="purL"
                     /locus_tag="ACHK3A_000661"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO8738795.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYVEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGASGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            8983..10410
                     /gene="qseE"
                     /locus_tag="ACHK3A_000662"
     CDS             8983..10410
                     /gene="qseE"
                     /locus_tag="ACHK3A_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO8738796.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIDLPSSKNTK"
     gene            10575..11288
                     /gene="qseG"
                     /locus_tag="ACHK3A_000663"
     CDS             10575..11288
                     /gene="qseG"
                     /locus_tag="ACHK3A_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO8738797.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            11278..12612
                     /gene="glrR"
                     /locus_tag="ACHK3A_000664"
     CDS             11278..12612
                     /gene="glrR"
                     /locus_tag="ACHK3A_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO8738798.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            12673..13011
                     /gene="glnB"
                     /locus_tag="ACHK3A_000665"
     CDS             12673..13011
                     /gene="glnB"
                     /locus_tag="ACHK3A_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO8738799.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(13056..14246)
                     /gene="hmpA"
                     /locus_tag="ACHK3A_000666"
     CDS             complement(13056..14246)
                     /gene="hmpA"
                     /locus_tag="ACHK3A_000666"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO8738800.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGLSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            14575..15828
                     /gene="glyA"
                     /locus_tag="ACHK3A_000667"
     CDS             14575..15828
                     /gene="glyA"
                     /locus_tag="ACHK3A_000667"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO8738801.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDASGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(15891..17083)
                     /locus_tag="ACHK3A_000668"
                     /pseudo
     CDS             complement(15891..17083)
                     /locus_tag="ACHK3A_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            17201..20482
                     /locus_tag="ACHK3A_000669"
     CDS             17201..20482
                     /locus_tag="ACHK3A_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO8738802.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNITLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVTKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKCSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            20579..21562
                     /locus_tag="ACHK3A_000670"
     CDS             20579..21562
                     /locus_tag="ACHK3A_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO8738803.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            21585..23096
                     /locus_tag="ACHK3A_000671"
     CDS             21585..23096
                     /locus_tag="ACHK3A_000671"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO8738804.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVSPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKDAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            23121..24119
                     /locus_tag="ACHK3A_000672"
     CDS             23121..24119
                     /locus_tag="ACHK3A_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8738805.1"
                     /translation="MSASSLLLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            24152..25246
                     /locus_tag="ACHK3A_000673"
     CDS             24152..25246
                     /locus_tag="ACHK3A_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO8738806.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            25258..26130
                     /locus_tag="ACHK3A_000674"
     CDS             25258..26130
                     /locus_tag="ACHK3A_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO8738807.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKNGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRNDDILC
                     LVYEHRSGVYHYRVSQTFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(26279..26701)
                     /locus_tag="ACHK3A_000675"
     CDS             complement(26279..26701)
                     /locus_tag="ACHK3A_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO8738808.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(26798..28000)
                     /gene="hcaD"
                     /locus_tag="ACHK3A_000676"
     CDS             complement(26798..28000)
                     /gene="hcaD"
                     /locus_tag="ACHK3A_000676"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO8738809.1"
                     /translation="MKEKTIIIVGGGQAAAMAAALLRQQGFSGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTQELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAASVMGRNAPPPVQRYLLPRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLRADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRIDNGALHRCESWENANNQAQIAAAAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     HGDDWICRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLID
                     EDIALKSL"
     gene            complement(28010..28822)
                     /gene="hcaB"
                     /locus_tag="ACHK3A_000677"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(28010..28822)
                     /gene="hcaB"
                     /locus_tag="ACHK3A_000677"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO8738810.1"
                     /translation="MSDLHNESIFITGGGSGLGLALAERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVNQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(28819..29139)
                     /locus_tag="ACHK3A_000678"
     CDS             complement(28819..29139)
                     /locus_tag="ACHK3A_000678"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO8738811.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(29139..29657)
                     /gene="hcaF"
                     /locus_tag="ACHK3A_000679"
     CDS             complement(29139..29657)
                     /gene="hcaF"
                     /locus_tag="ACHK3A_000679"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO8738812.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDGNWRLLERDIVLDQ
                     AVISSHNLSVLF"
     gene            complement(29654..31015)
                     /gene="hcaE"
                     /locus_tag="ACHK3A_000680"
     CDS             complement(29654..31015)
                     /gene="hcaE"
                     /locus_tag="ACHK3A_000680"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO8738813.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKHGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            31151..32041
                     /gene="hcaR"
                     /locus_tag="ACHK3A_000681"
     CDS             31151..32041
                     /gene="hcaR"
                     /locus_tag="ACHK3A_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO8738814.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            32201..33340
                     /locus_tag="ACHK3A_000682"
     CDS             32201..33340
                     /locus_tag="ACHK3A_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO8738815.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSSLTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(33332..34612)
                     /gene="csiE"
                     /locus_tag="ACHK3A_000683"
     CDS             complement(33332..34612)
                     /gene="csiE"
                     /locus_tag="ACHK3A_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO8738816.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRKSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(34803..35657)
                     /locus_tag="ACHK3A_000684"
     CDS             complement(34803..35657)
                     /locus_tag="ACHK3A_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8738817.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAEKAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(35802..36605)
                     /gene="suhB"
                     /locus_tag="ACHK3A_000685"
     CDS             complement(35802..36605)
                     /gene="suhB"
                     /locus_tag="ACHK3A_000685"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO8738818.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            36724..37464
                     /gene="trmJ"
                     /locus_tag="ACHK3A_000686"
     CDS             36724..37464
                     /gene="trmJ"
                     /locus_tag="ACHK3A_000686"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO8738819.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            37734..38222
                     /gene="iscR"
                     /locus_tag="ACHK3A_000687"
     CDS             37734..38222
                     /gene="iscR"
                     /locus_tag="ACHK3A_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO8738820.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            38334..39548
                     /gene="iscS"
                     /locus_tag="ACHK3A_000688"
     CDS             38334..39548
                     /gene="iscS"
                     /locus_tag="ACHK3A_000688"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO8738821.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            39576..39962
                     /gene="iscU"
                     /locus_tag="ACHK3A_000689"
     CDS             39576..39962
                     /gene="iscU"
                     /locus_tag="ACHK3A_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO8738822.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            39979..40302
                     /gene="iscA"
                     /locus_tag="ACHK3A_000690"
     CDS             39979..40302
                     /gene="iscA"
                     /locus_tag="ACHK3A_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO8738823.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            40398..40913
                     /gene="hscB"
                     /locus_tag="ACHK3A_000691"
     CDS             40398..40913
                     /gene="hscB"
                     /locus_tag="ACHK3A_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO8738824.1"
                     /translation="MDYFTLFGLPACYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            40930..42780
                     /gene="hscA"
                     /locus_tag="ACHK3A_000692"
     CDS             40930..42780
                     /gene="hscA"
                     /locus_tag="ACHK3A_000692"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO8738825.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            42782..43117
                     /gene="fdx"
                     /locus_tag="ACHK3A_000693"
     CDS             42782..43117
                     /gene="fdx"
                     /locus_tag="ACHK3A_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO8738826.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            43129..43329
                     /gene="iscX"
                     /locus_tag="ACHK3A_000694"
     CDS             43129..43329
                     /gene="iscX"
                     /locus_tag="ACHK3A_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO8738827.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            43388..44671
                     /gene="pepB"
                     /locus_tag="ACHK3A_000695"
     CDS             43388..44671
                     /gene="pepB"
                     /locus_tag="ACHK3A_000695"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO8738828.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGTDDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAARLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            44790..45566
                     /gene="sseB"
                     /locus_tag="ACHK3A_000696"
     CDS             44790..45566
                     /gene="sseB"
                     /locus_tag="ACHK3A_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO8738829.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(45598..45723)
                     /gene="timP"
                     /locus_tag="ACHK3A_000697"
     CDS             complement(45598..45723)
                     /gene="timP"
                     /locus_tag="ACHK3A_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO8738830.1"
                     /translation="MKIRCFCIVLIVSGALLSEVNNNRSLSGNNLLAVNNLQSSK"
     gene            complement(46385..47230)
                     /gene="sseA"
                     /locus_tag="ACHK3A_000698"
     CDS             complement(46385..47230)
                     /gene="sseA"
                     /locus_tag="ACHK3A_000698"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO8738831.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            47437..52398
                     /gene="yfhM"
                     /locus_tag="ACHK3A_000699"
     CDS             47437..52398
                     /gene="yfhM"
                     /locus_tag="ACHK3A_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO8738832.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQEFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNSARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGVEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTTSGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFTLWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDAMRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLSASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            52399..54711
                     /gene="pbpC"
                     /locus_tag="ACHK3A_000700"
     CDS             52399..54711
                     /gene="pbpC"
                     /locus_tag="ACHK3A_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO8738833.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWMHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLALLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAIPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLAEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            54860..55291
                     /gene="ndk"
                     /locus_tag="ACHK3A_000701"
     CDS             54860..55291
                     /gene="ndk"
                     /locus_tag="ACHK3A_000701"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO8738834.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            55441..56595
                     /locus_tag="ACHK3A_000702"
     CDS             55441..56595
                     /locus_tag="ACHK3A_000702"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO8738835.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            56880..57887
                     /gene="rodZ"
                     /locus_tag="ACHK3A_000703"
     CDS             56880..57887
                     /gene="rodZ"
                     /locus_tag="ACHK3A_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO8738836.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            57914..59032
                     /gene="ispG"
                     /locus_tag="ACHK3A_000704"
                     /gene_synonym="gcpE"
     CDS             57914..59032
                     /gene="ispG"
                     /locus_tag="ACHK3A_000704"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO8738837.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            59143..60417
                     /gene="hisS"
                     /locus_tag="ACHK3A_000705"
     CDS             59143..60417
                     /gene="hisS"
                     /locus_tag="ACHK3A_000705"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO8738838.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRTLGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            60435..61055
                     /gene="yfgM"
                     /locus_tag="ACHK3A_000706"
     CDS             60435..61055
                     /gene="yfgM"
                     /locus_tag="ACHK3A_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO8738839.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            61066..62244
                     /gene="bamB"
                     /locus_tag="ACHK3A_000707"
     CDS             61066..62244
                     /gene="bamB"
                     /locus_tag="ACHK3A_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO8738840.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            62362..63834
                     /gene="der"
                     /locus_tag="ACHK3A_000708"
     CDS             62362..63834
                     /gene="der"
                     /locus_tag="ACHK3A_000708"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO8738841.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEADDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            63897..64112
                     /locus_tag="ACHK3A_000709"
     CDS             63897..64112
                     /locus_tag="ACHK3A_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO8738842.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKRVEFIIA"
BASE COUNT        15174 a        16071 c        17758 g        15127 t
ORIGIN      
        1 cctggcagtg cgattaaagg agcagaggga ataaaaaacg ggggcatgac caacccccgg
       61 tacaaggaag gagtaactac aatggaaaaa ttaatgaaga cctttagtgc tacgttatat
      121 ggcgaataaa gactgccaca gggaagtttg cagaaacagt gatgccgatc gtgtttctga
      181 cgctcttctc tgaataagcg taatttaagt gaggtctgaa agtggatggc tgctcagtag
      241 aaaatgctaa agaaaggggg aggctttgtg ttgctgaact tatgcaaaat tttttttgca
      301 ccataaatcc aacaacacat tcaataacac attactgtat cactatgaaa ataaatgtgt
      361 ttttatatga tttcacacat atcacaattg atgcattttt tagtgattaa cagcgccata
      421 aggaaactct caaaacaaca caaaccgggc gggcattata cgcgcattct ttactcagac
      481 cagttttttc accagcgctt cactgtgacg aatacgttct ggagccagtt ccagatcctg
      541 ctgaatcagc gcaatgaaca gcaaatccgt taacattccc tgcgcgtgac aagcagagat
      601 tgaagcactg tttgtcgctt gttcttcggc aatggtatac aggcaatgag aagcacgctg
      661 ttgcagggca ttgggagtaa agccggtaat cgccagcact tttccgccca ctcgcagcat
      721 ctcatctgcc gccaggttta actcgcgtcg tacaccggtg taggaaatgg ctaataacag
      781 atcgtcaggg gacgacgcct gtactgtagc gagtagcgca tgcatatcgc gcactgcggc
      841 agcattgaag ccaatcttca tcagcttcca ggcaaagttt tgcgccacca gacccgaagc
      901 gccaataccg gtcagaatta tccgccgcgc agagcgcaac attgccacgc attcatgcag
      961 tttctcttca ctattaacgt ttaacgttgc gtacatagcg gcagtatttt ctttaatcag
     1021 tttttcgccg accagccgta acggatcatc accgcggatt tggttatgaa tgggcacgga
     1081 gggagattcc ggctggcttg ccagcgcttc actcaacgcc aacttaagtg ccggaaaacc
     1141 tttatagccg agtttttgcg cgaacttcac gacgctggac tgactgactc cggcttcgtt
     1201 ggccagttgc tgagagctta aatggcgcgc cgtatcaggt tgtagcagca gataatccgc
     1261 cagtttttta tcactttggg caagcccctg gtaacgctga cggatacgaa gtaagccgtt
     1321 catatctttc ctcgcgatga aatagtggcg acaacaggca atatgagaca cacgcgagtg
     1381 aattttatat tccattgccg ctaattgcca tgaattaaaa attccagtta gccatgatgc
     1441 ggtcgattgt tgccggaaaa gtgatgatct atctgttagg ctatgtagta gcgaaaatta
     1501 atcaggatgt ttcagtccag aggagtatgg tttggcaact cacgagcgtc gtgtggtgtt
     1561 ttttgactta gatggaacat tgcatcagca ggatatgttc ggcagttttc tgcgttattt
     1621 actgcgtcgc caaccgctga atgcgttact tgtcctgccg ttgttgccga tcatagccat
     1681 tgcgttattg ataaaaggtc gtgcggcacg ctggccgatg agtctgcttc tgtggggatg
     1741 cacttttggt cacaatgaag cgcgtttgca ggcgttgcag gccgatttcg tacgctggtt
     1801 tcgtgacaat gttaccgcct ttccgctggt tcaggagcga ttaaccacct acctgttaag
     1861 ttccgatgct gatatctggt tgattaccgg ctctccgcag ccgctggttg aagcggttta
     1921 tttcgatacg ccctggctgc cgcgggttaa tcttatcgcc agccaaattc agcgtggcta
     1981 tggtggttgg gtattgacga tgcgttgtct gggacatgaa aaggtcgcac aactggagcg
     2041 caaaatcggc acgccgctgc ggctgtacag tggctatagc gacagtaatc aggacaatcc
     2101 gctgctttat ttctgtcagc atcgttggcg agtaaccccg cgcggtgaac tccagcaact
     2161 ggaatagagt aaagcatagc gtccgtgtat aatgcgccgc gcttttataa ccggagtttt
     2221 ctttttgtct gaagtcgaat ttagccacga atactggatg cgtcacgcgc tgacgctggc
     2281 aaagcgtgcc tgggatgagc gggaagtgcc ggtcggcgcg gtattagtgc ataacaatcg
     2341 tgttattggc gaaggctgga accgtccgat tggtcgacat gatcccaccg cacatgcaga
     2401 aatcatggcc ctgcggcagg gcggtctggt gatgcaaaat tatcgactga tcgacgccac
     2461 gctgtatgtc acgcttgaac cctgtgtaat gtgtgccgga gcaatgatcc acagtcgcat
     2521 tggtcgcgtg gtctttggtg cgcgtgacgc gaaaactggc gctgcgggat ctttaatgga
     2581 tgtgctgcat catccgggta tgaatcaccg ggtggaaatt accgaaggaa tactggcgga
     2641 tgagtgcgcg gcgttgctca gtgacttctt tcgcatgcgc cgccaggaaa ttaaagcgca
     2701 gaaaaaagcg caatcctcga cagattaatt ttgtttctct tcactccctt ttctggaaaa
     2761 cagcagagag ggagagtggg tcaaatagtt tgatgacgac tgggaaagaa acgagagaaa
     2821 aggaaatttc tctttgccca gctccgtagg cgataccgcc ggataatcct gcgccagttg
     2881 catcgccgct tctgtagcct gcttctcttt ctcctgcaga taacccacca ggctaatctg
     2941 atacttacga atattttcga cataagcgta ggcttcatgc ccacgagcgt agccgtaagt
     3001 cagcttgctg taatagggtt tctggctaag taaaggcaga cgctgtttta cgtcagccca
     3061 actgtcagga ttacctttgg tttttgccgt cagggcgcgg gcatccagca tatgcgcata
     3121 gcccatattg tacgcggcaa gggcaaacca gatccgttcg ttctccggca cactttccgg
     3181 cactttactc atcatatcct gcaaataacg cacgccgccg ctgatgctct gttcggcatc
     3241 ggtacgatcc gtaatgccga ggctttgcgc ggtgtttttg gttaacatca tcatgccgcg
     3301 cacacccgtc ggtgaagtgg cctgagcatc ccagtgcgat tcttgataag caatagcggc
     3361 cagcaaacgc cagtcaattt cttcggcgta tttctcaaac aggggcttta actgcggcag
     3421 taccgcatcg acggcgcgta aaaatgtgcg cgtatcgacg taatcaaaat catcgccatg
     3481 ccccaggtat ttctcttcaa tgcgtgccag cgtaccgtct tcattcatct cattgaagaa
     3541 atcgagcaga gcggcggaaa gggtattatc gccatctaac gggctaaacc aggtcaccgg
     3601 ttgttcatcg gtgatatcga gcgctacggc gagttccggg tgaacgcgct gaaacaggct
     3661 gatggcgaca gaatcagcaa tggtgtaatc gagttttcct tcgatgacat cttccattaa
     3721 ttccgcagag ccttttttgt cgtctacctt ccagcttaat tccgggaatt ttgtttcttt
     3781 cagggtctgg agatcgttaa ccaccacatg acccggtgca acggtgagtt gctccgccgt
     3841 caggttgccc agtgtacgtg ggcgatactg acccacttta taaaccagtt gttgtgacac
     3901 ggaataatag gtagggccag gctgataatt ttttacacgc tcactgttat agaccaggcc
     3961 tgccgccagc aggtcggcgt taccattatc gaggtcgtca aacagctggc tgatattctg
     4021 ccgcacggtc actttcagtt ttacgccgag gtaatcggca aactgtttcg ccagttcgta
     4081 atccaggcca aaaggtttcc cgttgatttc gttataagtc aggggggtat gaatggtgct
     4141 cacacgcaac tctccccgcg cttgaatggc ggcgatacgg ttgtcggctt taccaaacca
     4201 gggaatggat ggccagagag cgaccgcgag cagcagtgcc agaatgccga tgaacagata
     4261 attaatcttt aattttttca attagttaat tctctgtgtc gtgcgcgtcc cagcttgaaa
     4321 aaacgtaata atagtgaaag gtttactcat aaatgagcgg cattttgcgt aaacctgcgc
     4381 cagttggcaa cttattacag ccattggcgg cacgcgttgc taattcacga tggtgatttt
     4441 atttccacgc aaacggtttc gtcagcgcat cagattcttt ataatgacgc ccgtttcccc
     4501 cccttgggta caccgaaagc ttagaagacg agagacttat gatggaaatt ctgcgtggtt
     4561 cgcctgcatt gtcggcattc cgaatcaaca aactgctggc acgttttcag gctgccaggc
     4621 tcccggttca cactatttac gccgagtatg tccattttgc tgacctcaat gcgccgttaa
     4681 acgatgatga gcacgcacaa cttgaacgcc tgctgaaata tggcccggca ctcgccagcc
     4741 acgccccgca aggcaaactc ctgctggtga ccccgcgtcc tggcaccatc tctccctggt
     4801 cttcgaaagc gaccgatatc gcccataatt gcggtctaca acaggtaaac cgccttgagc
     4861 gcggcgttgc ttactatgta gaagccggta cgctgaccaa tgaacaatgg cagcaggtta
     4921 ccgctgaact gcacgaccgc atgatggaaa cggtcttttt tgctttagat gatgcagagc
     4981 agctgtttgc acaccatcaa ccgactccgg tcaccagcgt tgatttgctg gggcagggcc
     5041 gtcaggcgct aatcgacgct aacctgcgtc ttggcctggc actggcggaa gatgaaattg
     5101 actatttgca ggatgctttc acaaagctgg gtcgtaaccc gaacgacatc gaactgtata
     5161 tgtttgccca ggcgaactcc gagcactgcc gccacaaaat ttttaacgcc gactgggtta
     5221 tcgatggtga acagcagccg aaatcactgt ttaagatgat caaaaacacc ttcgaaacca
     5281 cgccggatca cgttctctct gcttataaag ataacgccgc cgtaatggaa ggttctgaag
     5341 tgggccgcta ctttgctgac cacgaaacgg gccgctacga tttccatcag gaacctgcgc
     5401 atatcctgat gaaagttgaa actcacaacc acccgacggc gatttctccg tggccgggcg
     5461 cggcgaccgg ttccggcggt gaaatccgcg atgaaggtgc ctccgggcgc ggcgcgaagc
     5521 cgaaagctgg tctggtcggt ttctccgtgt ccaacctgcg aattcctggc ttcgaacagc
     5581 cgtgggaaga agatttcggt aaacctgagc gcattgttac cgcgctggac atcatgaccg
     5641 aaggcccgct gggcggtgcg gcgtttaaca acgaatttgg tcgtccggca ctgaatggct
     5701 acttccgtac ttatgaagaa aaagtgaaca gccacaatgg cgaagagctg cgcggttatc
     5761 acaaaccgat catgctggcg ggcgggatcg gcaacattcg tgccgatcac gtacaaaaag
     5821 gcgagatcaa cgttggtgcg aagctggtcg ttctcggcgg cccggcaatg aacatcggtc
     5881 ttggcggcgg cgcggcgtct tccatggcgt ctggtcagtc tgatgccgac ctcgacttcg
     5941 cttccgtaca gcgcgacaac ccggagatgg agcgtcgctg ccaggaagtg atcgaccgtt
     6001 gctggcagct tggtgatgcc aacccaatcc tgtttatcca cgacgttggc gcaggcggtc
     6061 tttctaacgc catgccggaa ctggtgagcg acggcgggcg cggcggtaaa ttcgaactgc
     6121 gcgatatcct gagcgacgaa ccgggcatga gcccgctgga aatctggtgt aacgaatccc
     6181 aggaacgcta cgtgctggca gttgctgccg atcagttacc gctgtttgac gaactgtgta
     6241 agcgtgagcg cgcgccatac gcggtgattg gtgaagcgac cgaagaactg catctttctt
     6301 tgcacgatcg tcattttgat aatcagccga tcgatctgcc gctggacgta ctgcttggta
     6361 aaacgccgaa gatgacccgc gaagtacaaa cgctgaaagc gaagggtgat gcgctggttc
     6421 gtgaagggat taccattgct gacgcggtga aacgcgtgct gcacctgccg accgtggcgg
     6481 agaaaacttt cctggtgacc attggtgacc gcagcgtaac cggcatggtg gcgcgcgatc
     6541 agatggtggg tccgtggcag gtgccggtcg ctaactgcgc ggtcactacc gccagcctcg
     6601 atagctacta cggtgaagcg atggcgattg gcgagcgtgc gccggttgcg ctgctggatt
     6661 tcgccgcttc tgcccgtctg gcggtcggtg aagcgttaac caacatcgcc gcaacacaaa
     6721 ttggcgatat caaacgcatt aaactttccg ccaactggat ggcggcggca ggccaccctg
     6781 gtgaagatgc aggcctgtat gaagccgtta aagccgtggg tgaagaactt tgtccggcgc
     6841 tgggcctgac gatcccggtg ggtaaagact ctatgtcgat gaaaacccgt tggcaggaag
     6901 gtaacgaaga gcgcgaaatg acgtcgccgc tgtcgctggt gatttctgca tttgcccgcg
     6961 tggaagatgt acgtcacacc atcacgccac agctttctac cgaagataac gcactgctgc
     7021 tgattgattt gggcaaaggc aataacgcgc tgggcgcaac ggcgctggcg caggtttatc
     7081 gtcagcttgg cgacaaaccg gcagatgtac gcgatgtcgc gcaactgaaa ggcttctatg
     7141 acgcgattca ggcgctggtt gcacagcgta agctgctggc gtatcacgac cgctctgatg
     7201 gcggcctgct ggtaacgctg gcggaaatgg cctttgctgg tcattgcggc attgacgcgg
     7261 atatcgcctc tctgggtgac gatcgcctgg cggcgttgtt taacgaagaa ctgggtgcgg
     7321 tgattcaggt tcgtgccgct gaccgtgaag ccgttgaagc ggtactggca cagcatgggc
     7381 ttgcggattg cgtccattat gtagggcagg cggtttccgg tgaccgtttt gtgattaccg
     7441 ccaacgggca gactgtattc agcgaaagcc gcactacgtt gcgtgtctgg tgggcagaaa
     7501 ctacctggca gatgcagcgc ctgcgtgaca acccggagtg tgccgatcag gagcaccagg
     7561 cgaaatcaaa cgacgccgat ccgggcctga atgtgaaact gtcgttcgat atcaacgaag
     7621 atgtggcagc accgtatatt gccactggtg cgcgtccgaa agttgccgtg ctgcgtgagc
     7681 agggggtgaa ctcgcatgtt gaaatggcgg cagctttcca ccgtgcaggc ttcgatgcaa
     7741 tcgatgtgca tatgagcgac ctgctggctg gacgcacggg cctggaggat ttccacgctc
     7801 tggtagcgtg cggtggattc tcctacggtg acgtgctggg tgctggtgaa ggctgggcga
     7861 agtctatcct gttcaatgac cgtgtacgcg atgagtttgc aaccttcttc caccgtccgc
     7921 aaacgctggc gctgggggta tgtaacggtt gccagatgat gtctaaccta cgcgaactga
     7981 tcccaggtag tgagttgtgg ccacgttttg tgcgcaatac ctccgatcgc tttgaagcgc
     8041 gtttcagcct ggttgaagta acccaaagcc cgtctctgct gttgcagggg atggtgggct
     8101 cgcaaatgcc gattgctgtc tctcatggcg aagggcgcgt ggaagtgcgt gatgcggcgc
     8161 atctggcggc actggaaagc aaagggctgg tggcactgcg ctatgtcgat aacttcggca
     8221 aagtcactga aacctacccg gctaacccga acggttctcc gaatggtatt acggcagtca
     8281 cgactgaaag tggtcgagtc accattatga tgccgcaccc ggaacgtgtt ttccgtactg
     8341 tcagcaactc ctggcatccg gaaaactggg gcgaggatgg gccgtggatg cgtattttcc
     8401 gcaatgcacg taagcagttg gggtaagccg tcaacccatt ggttttgcag ccggatgagg
     8461 cattaagcac caactactat tataagctcc caacttcggg agcttttttg tgtctgtaaa
     8521 tcacgacaat gggtggttta ccgcgtcgct ttctggcgac acttaactaa ttgattttaa
     8581 tggcatctaa taagtttatc ttaagttgtc tctttttagc gacacagtgg ctgatttaca
     8641 gccaaatagc gaagaagcca taaccccttc aaaacagcgt acaatcagta cgttaccaaa
     8701 ctattttctt tattggcaca gttactgcat aatagtaacc agtggctcat tcaccgactt
     8761 atgtcagccc cttcgggacg tgctacataa aatacgaatg acgcacaaca aggtgcctgc
     8821 cgtccaactt ctgatatcag cgtagctata tcaaccatcg ggcgaaacgt cgagttaggc
     8881 accgccttat tccataacaa agccgggtaa ttcccggctt tgttgtatct gaacttcccc
     8941 tcggttagca tcaggctatt cgcgtctgac gagagtaaca ccttgaaacg ctggcccgtt
     9001 tttccccgct cattacgaca actggtaatg ctggcatttt tgctgattct gctgcccctg
     9061 ttggtgctgg catggcaagc ctggcaaagc ctgaatgcgc ttagcgatca ggcggcgctg
     9121 gttaaccgca ctacgcttat tgatgcccgg cgtagtgaag cgatgaccaa cgcggcgctg
     9181 gagatggagc gtagctaccg tcagtattgc gtgctggacg accctacgct tgcgaaggtt
     9241 tatcaaagcc agcgcaagcg ttacagcgaa atgctcgatg cccacgcagg cgtactgcca
     9301 gacgataaac tttaccaggc attgcgtcag gatttgaaca atctggccca acttcagtgt
     9361 aacaacagtg gtcccgatgc tgccgccgcc gcgcgtcttg aagcctttgc cagtgccaat
     9421 accgaaatgg tgcaggccac gcgcacagtg gtgttctctc gtgggcagca acttcagcgt
     9481 gaaatcgccg aacgtgggca atattttggt tggcaatcgc tggtgctatt tctggtgagt
     9541 ctggtgatgg tgctactttt cacgcggatg attatcgggc cggtgaaaaa tatcgagcgc
     9601 atgatcaacc gtctggggga agggcgttct ctgggcaata gcgtttcgtt cagtggaccg
     9661 agcgagttac gctcggttgg gcaacgtatt ctttggttaa gtgagcgcct gtcatggctg
     9721 gaatcccaac gccatcaatt tttaagacat ttatctcatg aattaaaaac gccactggcg
     9781 agtatgcgcg agggcactga attactggct gaccaggttg tcgggccgct tacgccagag
     9841 caaaaagagg tggtgagcat tcttgatagc agcagccgca atttgcaaaa actgatcgaa
     9901 caactgcttg attacaaccg taaacaggcg gacagtgcgg tggaactgga gaatgttgaa
     9961 ttagcaccgc tggtggagac agtggtttct gctcatagcc tgcccgcacg ggctaaaatg
    10021 atgcataccg acgtcgatct caaagcaaca gcttgcctgg cggagccaat gctgctgatg
    10081 agcgtactgg ataatcttta ctccaatgcg gtgcactacg gggctgaatc cggtaacatt
    10141 tgccttcgca gcagttcaca tggtgcgcgg gtttatattg atgtcatcaa tacaggcacg
    10201 cccattccgc aagaggaacg cgccatgatc ttcgaaccct tttttcaggg aagccaccag
    10261 cgaaaagggg cggtgaaggg cagcggtctg ggattaagca ttgccaggga ttgtattcgc
    10321 cgtatgcaag gggaactgta tctggtcgac gagagcgggc aagacgtttg tttccgcatt
    10381 gatttaccgt cgtcgaaaaa cacgaaataa tgctaacagc ccatcccatc aggctatttt
    10441 atttgtcagt tcggccccgg gcagtgctcg aaatcttcac gtacgacatg tacgctctgg
    10501 tttctccgcg ctgtccatgt ccaaactgcc tgcaacaatt acgcccactg ggataggctt
    10561 taagtctggt gaatatgcga cacatttttc aacgattatt gccccgacgg ctgtggctgg
    10621 cagggctgcc atgtctggca ttgctgggtt gcgtgcagag tcacaataag ccagccattg
    10681 atacgccagc agaagaaaaa attccggttt atcaactggc tgactatctt tctactgaat
    10741 gtagcgacat ctgggcgctg caaggtaaat caaccgaaac caatccgctt tactggctgc
    10801 gggcgatgga ttgtgctgat cgtttaatgc ctgcgcagtc gcgtcagcaa gcccgccaat
    10861 atgacgacgg caactggcaa aataccttta aacaagggat tttgctcgcc gacgccaaaa
    10921 ttacgccgta tgagcgccgc cagctggtcg cgcgcattga tgcgctaagt accgagatcc
    10981 cggcacaggt tcgccctctg tatcaactct ggcgcgacgg tcaggcgctg caactgcaac
    11041 tggcggaaga gcgccaacgc tacagtaaac tccagcagtc cagcgacagc gagctggata
    11101 ctttgcgcca gcaacatcac gttctacaac agcaactgga actcactacc cgcaagctgg
    11161 aaaacctgac cgatattgaa cgccagctct cgacccgcaa accggctgga aatttctcgc
    11221 cggatacgcc gcacgagagt gaaaagcccg cgccatccac tgatgaggtc actcctgatg
    11281 agccataaac ctgcgcattt attactggtc gatgacgatc cgggattgct gaaactgctt
    11341 ggcctgcgcc tgaccagcga aggctacagc gtggtcacgg cggaaagtgg tgctgaagga
    11401 ttacgggtac tgaatcgcga aaaagtagat ttagttatca gcgacctgcg gatggatgaa
    11461 atggatggta tgcagctgtt tgctgaaatc cagaaagtgc agccgggaat gccggtaatt
    11521 attcttaccg cacatggttc tattcccgat gccgttgctg caacacagca gggagtgttc
    11581 agtttcctca ccaagcctgt cgataaagac gcgctatatc aggcaattga cgatgcgctg
    11641 gaacaatccg cgccagccac cgatgaacgc tggcgcgagg cgattgtcac ccgcagcccg
    11701 ctgatgctgc gtttgctgga acaggcgcgg ctggtggcgc aatcagacgt tagcgttttg
    11761 attaacggtc agagcggcac cgggaaagag attttcgccc aggctatcca caacgccagc
    11821 ccgcgcaata gcaaaccatt tattgctatt aactgtggtg cattgcccga gcaattgctg
    11881 gagtcggagc tgtttggtca tgcgcgtggc gcgtttactg gcgctgtcag caatcgcgaa
    11941 ggtttattcc aggcggcgga aggcggtacg ctatttctcg atgagattgg cgatatgcct
    12001 gcaccgttgc aggtcaaact gctgcgtgtg ttgcaggagc gtaaagtgcg cccgctgggc
    12061 agtaaccgcg atattgatat cgacgtgcgg attatttccg ccactcaccg cgacctgcca
    12121 aaagcgatgg cgcgcgggga attccgtgaa gatctctatt accgcctcaa cgttgtcagc
    12181 ctgaaaattc cggctctggc agagcgcaca gaagacattc cgctactggc aaatcacctg
    12241 ttgcgccagg cggcagagcg acataaaccg tttgtccgcg cgttctccac cgatgcgatg
    12301 aaacgcctga tgaccgctag ctggccgggt aatgtacgcc agttggtcaa cgtgattgaa
    12361 cagtgcgtgg cgctgacctc atctccggtg attagcgatg cattagtaga gcaggcgctg
    12421 gagggcgaaa acacggcgct gccaaccttt gttgaggcac gtaatcagtt tgaactcaac
    12481 tatttgcgta agctgctgca aatcaccaaa ggcaacgtca cccacgcggc gagaatggcg
    12541 gggcgcaacc ggacagaatt ttataaactg ctttcccgac acgagctgga tgcaaacgat
    12601 ttcaaggaat gaattggcgt tatgtgttac gtttagcaga tcaaaagaca ggcgaccttt
    12661 tcaaggaata gcatgaaaaa gattgatgcg attattaaac ccttcaagct ggacgatgtt
    12721 cgcgaagcac tggctgaagt cggcattacc ggcatgacgg tcactgaggt gaaaggtttt
    12781 ggccgccaga aaggccatac cgagctgtac cgcggcgcgg agtatatggt ggattttctg
    12841 ccgaaagtga aaattgagat tgtcgtaccg gacgacattg tcgatacctg tgtcgatacc
    12901 attattcgca cggcgcaaac cggcaaaatc ggtgacggta aaatcttcgt ctttgacgtg
    12961 gcacgggtca ttcgtatccg taccggtgaa gaagacgacg cggcaattta aaccgcgtca
    13021 agggttgccg gatgtttcca tccggcaaca tcaaatcaca gcaccttatg cgggccaaag
    13081 cattcgtaat gaatattttc ctgcttcacg cccagatcca ctaactgttt cgcggcaaac
    13141 tgcatgaagc caaccgggcc gcagagatag aactgcattg tcggatcgct gaacgcacct
    13201 tccagtttgc tcaaatccat cagaccttcg ctatcaaact gacctttagc gcgatcggct
    13261 tcgctcggct gacgatacca ggtgtgcgcg gtaaagcgcg gcagtgacag ccccagttcc
    13321 ttaacttcat cagcaaaggc gtgaacatcg ccattttctg ccgcatggaa ccagttcact
    13381 tgtgctgtgt ggcctgcttt tgccagtgtg tcgagcattg ccagcattgg cgtttgaccg
    13441 acaccggcag agattaacgt cactggtgtg tcatctgcga cagccataaa aaaatcacct
    13501 gctggagcga ccagtttcac gacatcgcca acattggcgt gattgtgcag ccagttggat
    13561 acctgcccac cctcttcgcg tttcaccgca atacgatagc ctttgccatc cggtttgcga
    13621 gtcaaagagt actgacgaat ttcctgatgc gggaaacctt ccggcttcag ccagacgccg
    13681 agatattgcc ccggacggta ttctgccact gcgccaccgt cgaccggctc cagttcgaag
    13741 ctggtgataa gcgcgctgcg cggtgtttta gccacaatgc ggaaatcgcg agtaccttcc
    13801 cagccaccgg ctttgctggc gttttcgtta tagatttccg cctcgcgatt gataaataca
    13861 ttagccagta cgccataggc tttaccccac gcgtccagca cttcctggcc cgggctgaac
    13921 atttcgtcca gcgtcgccaa caggtgttca ccgacaatgt tgtactgttc cggtttgatc
    13981 tggaagctgg tgtgcttctg cgcgattttt tctaccgctg gcagcagcgc aggcaggttt
    14041 tcaatattac tggcgtaggc ggcaatagcg ttaaacaggg cttcacgttg atcgccattg
    14101 cgctggttac tcatgttaaa aatttctttg agttctgggt tatgagtaaa catacggtcg
    14161 tagaaatggg cggttaactt tggtcccgtt tccaccagta aagggatggt ggcttttact
    14221 gtagcgatgg tttgagcgtc aagcatatgg tcttcctttt ttttgcatct taattgatgt
    14281 atctcaaatg catcttataa aaaatagccc tgcaatgtaa atggttcttt ggtgtttttc
    14341 agaaagaatg tgatgaagtg aaaaatttgc atcacaaacc tgaaaagaaa tccgtttccg
    14401 gttgcaagct ctttattctc caaagccttg cgtagcctga aggtaatcgt ttgcgtaaat
    14461 tcctttgtca agacctgtta tcacacaatg attcggttat actgttcgcc gttgtccaac
    14521 aggaccgcct ataaaggcca aaaattttat tgttagctga gtcaggagat gcggatgtta
    14581 aagcgtgaaa tgaacattgc cgattatgat gccgaactgt ggcaggctat ggagcaggaa
    14641 aaagtacgtc aggaagagca catcgaactg atcgcctccg aaaactacac cagcccgcgc
    14701 gtaatgcagg cgcagggttc tcagctgacc aacaaatatg ctgaaggtta tccgggcaaa
    14761 cgctactacg gcggttgcga gtatgttgat atcgttgaac aactggcgat cgatcgtgcg
    14821 aaagaactgt tcggtgctga ctacgctaac gtccagccgc actccggctc ccaggctaac
    14881 tttgcggtgt acaccgcgct gctggaacca ggtgataccg ttctgggtat gaacctggcg
    14941 catggcggtc acctgactca cggttctccg gttaacttct ccggtaaact gtacaacatc
    15001 gttccttacg gtatcgatgc tagcggtcat atcgactacg ccgatctgga aaaacaagcc
    15061 aaagaacaca aaccgaaaat gattatcggt ggcttctctg catattccgg cgtggtggac
    15121 tgggcgaaaa tgcgtgaaat cgctgacagc atcggtgctt acctgttcgt tgatatggcg
    15181 cacgttgcgg gcctggttgc agctggcgtc tacccgaacc cggttcctca tgctcacgtt
    15241 gttactacca ccactcacaa aaccctggcg ggtccgcgcg gcggcctgat cctggcgaaa
    15301 ggtggtagcg aagagctgta caaaaaactg aactctgcgg ttttccctgg tggtcagggt
    15361 ggtccgttga tgcacgtaat cgccggtaaa gcggttgctc tgaaagaagc gatggagcct
    15421 gagttcaaaa cttaccagca gcaggtcgct aaaaacgcta aagcgatggt agaagtgttc
    15481 ctggaacgcg gctacaaagt ggtttccggc ggcactgata accatctgtt cctggttgat
    15541 ctggttgata aaaacctgac cggtaaagaa gctgacgccg ctctgggccg tgctaacatc
    15601 accgtcaaca aaaacagcgt accgaacgat ccgaagagcc cgtttgtgac ctccggtatt
    15661 cgcgtgggta ctccggcgat tacgcgtcgc ggcttcaaag aagcagaagc gaaagaactg
    15721 gctggctgga tgtgtgacgt gctggacagc atcaatgatg aagccgttat cgagcgcatc
    15781 aaaggtaaag ttctcgacat ctgtgcacgt tacccggttt acgcataagc gaaacggtta
    15841 atctgttgaa aaaggcttcc ttcgggaagc ctttctacgt tatcgcgcca tcaaatctgt
    15901 cgtaactgcg cctcaacata caaatagcca atccccagca cctgttgtgc gcggcttaat
    15961 tgcccaaagc caattttcgt cgctttcacg ctcggtcctt cgtcgcggtc gaacgggtta
    16021 aatcccgtct ggcgaataat ggtattaagc cagtgctcac caaggcacaa ctgcgaccgt
    16081 acaaccaaat ctgattaata ttgaggatgt tgaggaagtt atacagactc aaaccaatgg
    16141 cattggcaga gcgatcaacc cagctggtga tccacggttc tccactctgc caggcagcga
    16201 ttaactgcgc tgttgtcagt ttttcaggat caagctgagt attaaccggt tgtgatttta
    16261 gccatacccg cgcctgtttt tttaatgcac ttaacgaggc gacggtttcc aggcagccgt
    16321 aacgtccaca gtcgcagacg acgccatccg gattaacgat ggtgtgacca atctgtccgc
    16381 taccatacaa acagccgcga taaatttgct cgttaatcac gaacgacgag ccaatgccgt
    16441 aatcaacgtt gatcacgcag aaatcccgtt cctgcgaatt attttgccat ttctccgcca
    16501 gcgccagcat cacgcagtca ttatcgacca tcacccgaat gccgagcttc tcttccagca
    16561 gatactttac ctcaaccggc gttgtccacg gcgcttgcgg catggtttgc gacacgccgg
    16621 tcaccggatc aacctgaccg tggattgcca gcgccaggtt gatggtacgg tccggccaca
    16681 atttacggtg gcgatgccag catttttcga tttcggacag cagcgcctgc ggcgtcggtg
    16741 catcaatctg taaatactca aattcacctt tcggacttaa acaagcatta gcaacctgac
    16801 actcaatact ggtgggcgtc acgttcaggc acaacgtcca gtcaccttcc ggcgcaatca
    16861 gccatgtacc gctactatgc ccgcgcgtct ggctttcatc gtcgatattc accacccgtt
    16921 tttcgctttc cagttcctgc aaaatattac tgaccgccgg aatcgaaatt tgcgccagcc
    16981 gggccagcgt tgatttattg gcgcgctttt gccggtacag cagttccaga atcacgcatt
    17041 tgttatggtg gcgaatctgt tgattattaa tgcaggctct catcttcact aaaccttgtt
    17101 tagttaattg cgtgattatt ccgcgaaaac attgccagat tgttcaatac actgccacaa
    17161 atcttttaat tcagtatgtc ttgttaatat tgagggcacc atgactccag taaaagtgtg
    17221 gcaagagcgc gttgagatcc cgacctatga aaccgggccg caggatatac atcccatgtt
    17281 cctggaaaat cgcgtttatc agggatcgtc cggcgcggtt tatccctacg gcgtgaccga
    17341 tacgctgagc gagcagaaaa ccctgaaatc ctggcaggcg gtgtggctgg aaaacgacta
    17401 catcaaagtg atgatcctgc cggagctggg cggtcgggtg catcgcgcgt gggataaagt
    17461 gaaacagcgc gattttgtct atcataatga agtcattaaa ccggcgctgg tggggttgct
    17521 ggggccgtgg atctccggcg ggattgagtt taactggccg caacaccatc gcccgaccac
    17581 ctttatgccc gtcgatttca ccctcgaagc ccatgaagac ggcgcacaaa ccgtgtgggt
    17641 cggcgaaacg gagccgatgc acggtttaca ggtgatgaca ggtttcaccc tgcgccctga
    17701 ccgggcggca ctggaaattg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt
    17761 cttgtggtgg gccaacccgg cagtgaaagg gggtgaaggg catcagagcg tcttcccgcc
    17821 ggatgtaacg gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgccac
    17881 cggcacttac tacaaagtgg actactccgc cggagtggac atttctcgct ataaaaatgt
    17941 gcccgttcca acctcatata tggctgaaaa atcacagtac gattttgttg gcgcgtggtg
    18001 tcacgatgaa gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa
    18061 acagtggagc tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa
    18121 taacggcccg tatatcgaac tgatgactgg tatttttgcc gataaccagc ccgattttac
    18181 ctggcttgat gcttacgaag agaagcgttt tgagcagtat ttcctgcctt accattcttt
    18241 gggcatggtg caaaacgcct cccgcgatgc ggtgattaaa ctccagcgta gcgagcgggg
    18301 gattgagtgg gggctgtatg ccatctctcc gttgaaagga taccgtctgg cgatccgcga
    18361 aatcggcaaa tgcaacgcgt tgctcgatga tgccgtggcc ctgacaccag cgaccgccat
    18421 ccagggcgtg ttgcacggta tcaatcctga aaggctgacc attgaactct ccgatgccga
    18481 cggcaatatc acactgagtt atcaggaaca tcagccgcaa gagttgccgt tgccggacgt
    18541 cgccaaagcg ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg
    18601 tcagcatctg gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg
    18661 cgtggcgctg gatccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa
    18721 ccgagcagat ttcccgcaag cggtggcgta tgccactcag gcgctgaaac gcgcacatgc
    18781 gctaaacaaa aatccgcagt gcggacaggc gagtttgatt cgcgccagtg cttacgaacg
    18841 tcaggggcaa tatcaacaag ccgaagagga tttctggcgg gcggtctgga gcggcaacag
    18901 taaagcaggt ggctattatg gtctggcacg gctggcggcg cgtaatggtc acttcgacgc
    18961 tggtctggat ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg
    19021 cctgcataat ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcga
    19081 gaaactgctg cgtgattatc cgctgaacgc cactctgtgg tggctgaact ggttcgatgg
    19141 tcgtagcgaa tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc
    19201 cctgatgacc gccgggcaac tgattaactg gggaatgccc accctggcgg cagagatgct
    19261 gaacgcactg gactgccagc gcacgctgcc gctttacttg caagccagct tgctgccgaa
    19321 agctgaacgc ggcgaactgg tcacaaaagc cattgatgtc ttcccacagt ttgtccgttt
    19381 cccgaatacg ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg
    19441 tcatttactg gcctgcttct actacaacaa gtgcagctac aacaaagcca ttactttatg
    19501 gcaacgttgc gtagagatgt cgccggagtt tgccgacggc tggcgcgggt tagcgatcca
    19561 tgcgtggaat aagcaacacg attatgagct ggccgcgcgt tatcttgata atgcttatca
    19621 gcttgcgccg caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagcgg
    19681 tgtcacaccg gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg
    19741 cgacgacatg accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc
    19801 ggcggacatt ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaagatcac
    19861 cagtcagttt atcctcaacc agttattacg cgcctggcag catcttgatg ccagagagcc
    19921 gcagcaggcc agcgaactgc ttcatgccgc gttgcattat ccggagaatt taagcgaagg
    19981 ccgtttaccg gggcaaactg acaacgacat ctggttctgg caggcgatat gcgccaacgc
    20041 gcagggcgat gaaactgaag cgacgcgttg tttacgtctg gcggcgactg gcgatcgcac
    20101 cattaacatt cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat
    20161 ggcgctgcga ctgctgggcg aacaacacac cgcacagcaa ctgtttagtg aaatgaaaca
    20221 gtgggcgcaa gagatggcga aaaccagtat cgaggcggat ttctttgctg tttcacaacc
    20281 tgacctgttg tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt
    20341 ggcgatgctg gcgtccgcgg gactcgggga ggttgcgcac tatgaatctg ctcgcgctga
    20401 attgatggcg attaatccag cctggccgaa agcggcatta ttcaccaccg tgatgccttt
    20461 tatttttagc tacgttcact aaaccttatt aactgtaata tttgagcggc acgcgctgcc
    20521 gctcacgctc aaataattaa aataagccgt tgtaaataaa aaccctacag gaggcactat
    20581 gcctaaaaaa atgacaagaa cccgtaattt attattaatg gctacgctat tgggtagcgc
    20641 actatttgcc agggctgcgg ataaagaaat gactattggc gcaatatacc ttgataccca
    20701 gggatattac gccggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca
    20761 ggtacagtta attgaaacta acgctcaggg cgatatttcg aaagaaagta cctttgttga
    20821 taccctcgtg gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag
    20881 tagtcgtacc gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg
    20941 tattaatcaa aaaggtgtcg ataaatatgt ctcggcgtat ctggtcggcg atccgctgga
    21001 atttggtaaa aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca
    21061 gccgaaaatt gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg
    21121 atttgaagag gtattaaaat cccgcgttcc cggcgcgcaa atagtcgcta atcaggaagg
    21181 gactgtttta gataaagcga tttccgttgg tgaaaaacta attatctcca cgccggatct
    21241 caacgccatt atgggggaat cgggcggtgc gacactcggc gcggtaaaag cggtacgtaa
    21301 tcaaaatcag gccggaaaaa ttgctgtttt cggttcggat atgaccactg aaattgctca
    21361 ggagctggaa aacaatcagg tgctgaaagc ggtggtggat atttccggta agaaaatggg
    21421 caatgctgtt ttcgcgcaaa ctttgaaggt tatcaataaa caagccgacg gtgaaaaagt
    21481 gattcaggtg ccaatcgatc tctataccaa aacggaagac ggtaaacagt ggctggcaac
    21541 gcacgttgat ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acagcaacag
    21601 aggcagtccc ggtagcaaaa gtggtggcag gaaataagcg ttatcccggc gtcgttgcgc
    21661 tggataacgt taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggcaaaaacg
    21721 gtgcgggaaa atcgaccctc attcgaatgc ttaccggtag cgaacgtccg gatagcggtg
    21781 atatctggat tggcgagacg cgactggaag gtgacgaagc tacgctgact cgccgtgccg
    21841 ctgaactggg ggtacgcgcg gtttatcagg agttaagtct ggtggaaggg ctgacagtgg
    21901 cggaaaacct ctgcctcggt cagtggcccc gccgcaacgg catgattgat tacctgcaaa
    21961 tggcgcagga cgcccaacgt tgcttgcagg cgttgggcgt tgacgttagt cctgaacaac
    22021 ttgtttcaac actaagcccg gcgcaaaagc agctggtgga aattgcgcgg gtgatgaagg
    22081 gcgagccgcg cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcggaggttg
    22141 aactggtgat cagcgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca
    22201 gccaccggat ggaagaaatt cgccgcattg cctcctgtgc caccgttatg cgcgatggtc
    22261 aggtggcggg cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc
    22321 tcgggcgcga tcacgttgat attgcgccgg tttcccctca ggaaattgtc gatcaggccg
    22381 tgctggaagt ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgctcc
    22441 gtcgtggcga agtactcggc attgctggcc tgctgggggc agggcgcagt gaattgttga
    22501 aagccatagt tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa
    22561 tcactcgccc cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc
    22621 gcaaagatgc ggggatcatt ccctggctgg gcgttgacga aaatacagtg ctgaccaatc
    22681 ggcaaaaaat cagcgccaac ggtgtgctgc aatggtccac catccgccgc ctgaccgaag
    22741 aggtgatgca gcggatgacg gtcaaggccg ccagtagcga aacgcccatc ggcacgcttt
    22801 ctggcggcaa tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt
    22861 tgttgctcga cgagccaacg cgtggcgtcg atatcgaagc caaacagcag atttaccgta
    22921 ttgtccgcga gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg
    22981 agttgccgct ggtgtgcgac cgcattctgt tgttacagca cggtacgttc tcgcaggagt
    23041 ttcacgctcc ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa
    23101 ttttaccgag gaacctgaag atgtctgctt cgtcattact attgccgcag ggcaagagcg
    23161 tctcgctcaa acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgatcg
    23221 ccattcttta tctggtcttc tctctgaacg cgcctggctt tatctcattg aataaccaga
    23281 tgaacgtgct gcgcgatgcc gccaccatcg ggatcgccgc atgggcgatg acgctgatta
    23341 ttatctccgg tgaaattgat gtcagcgttg gtccgatggt ggcttttgtc tcggtgtgcc
    23401 tggcattttt gctgcaattt gaagttccgc tggcggtggc gtgtctgctg gtgttgctgt
    23461 taggcgcgct gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtgccaagtt
    23521 ttgttgccac gctgggttta tggagcgccc tgcgcggaat ggggctgttt atgaccaacg
    23581 ccttgccagt gccgattgat gaaaacgaag tgctggactg gctgggggga caatttctcg
    23641 gtgtgccggt atccgcgctg atcatgatgg tattgtttgc gctgtttgtg ttcatcagcc
    23701 gcaaaaccgc cttcgggcgc tcggtttttg ccgttggcgg taatgccact gcagcacagt
    23761 tgtgcggtat taacgttcgt cgggtacgca ttcttatctt taccctttcg ggattattag
    23821 cggcggtgac cggcattttg ttggcggcgc gcctcggttc cggtaacgca ggtgccgcaa
    23881 acggtctgga gtttgacgtc atcgccgcgg tcgtggttgg cggtacggcg ctttccggtg
    23941 gtcgcggctc gttgttcggt acgctgcttg gcgtactggt gattacgcta atcggtaacg
    24001 gtctggtgct gctcgggatt aactcctttt tccagcaggt ggtgcgcggc gtcatcatcg
    24061 tggtggcggt gctggcgaat atcttgctga ctcagcgtag cagtaaagcg aaacgctaaa
    24121 ccttaacgcc cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg
    24181 atttatgaaa acgatgctgg cagcttattt accaggaaat tcgaccgtcg atctgcggga
    24241 agttgcggtg ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat
    24301 ttgcggaagc gatgtccact atatctatca tcaacaccgt gccacggcag cggcacccga
    24361 taaaccgtta taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat
    24421 ggggcaaggc tgccgccatt ttaaagaggg cgaccgtgtg ctggtgtatc acatttctgg
    24481 ctgtggtttt tgcccgaact gccgtcgcgg ctttcctatc tcttgtactg gcgaaggaaa
    24541 agcggcttac ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa
    24601 agatctgatc ctcctgccgg atgcactgag ttacgaagat ggtgcgttta tcagttgcgg
    24661 cgttggtaca gcgtatgaag ggattttgcg cggcgaagtt tccggcagtg ataacgtact
    24721 ggtggtcggt ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc
    24781 aaaaaggatc atcggcgttg atatgctgcc ggaacgtctg gcgatggcga aacagttagg
    24841 agtgatggat cacggctatt tagcgaccac cgaaggtctg ccgcagataa tcgccgaact
    24901 cactcacggc ggcgcggatg ttgcgcttga ttgttccggt aatgccgcag gtcgcttact
    24961 ggcactgcaa tccaccgctg actggggacg cgtggtttac attggtgaaa ccggaaaagt
    25021 cgagttcgag gttagcgccg acctgatgca ccatcaacgg cggattatcg gctcctgggt
    25081 gaccagtctg ttccatatgg aaaaatgcgc ccacgattta acggactgga aactgtggcc
    25141 gcgtaacgcc attacccacc gcttctcgct ggaacaggcg ggagatgctt atgcactgat
    25201 ggcaagcggc aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg
    25261 accatctata ccttatccca cgggtcgcta aagctgggcg tttctgacca gggcggcgta
    25321 atcgaaggct tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aaacggtgtg
    25381 gcaaccgatg cctcttgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt
    25441 tttgtctggc aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat
    25501 ctgcacggcg acggctggct ggggcagtgg caatgtgtct cgcgcaatga tgatatcttg
    25561 tgtctggtgt atgaacatcg cagcggtgtt tatcactatc gggtaagcca gacgtttcat
    25621 ttaacggcgg atacgctgac ggtgacactc tctgtcacca atcaaggggc agagacgctg
    25681 ccatttggta ccggctggca tccttatttt ccgttgtcgc cgcaaacgcg gattcaggca
    25741 caggcgagcg gttactggct ggagcgggag cagtggctgg cgggtgagtt ttgcgagcag
    25801 ctaccgcaag aattggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat
    25861 ggttttgccg gatggaatgg tcaggcccgc atcgagcagc cgcaggaggg ttatgccatc
    25921 atcatagaaa cgacgccacc tgcaccgtgt tatttcatct ttgtttccga cccggcgttt
    25981 gataaaggat atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccagatgac
    26041 catcatcgcc cggaaggtgg tgacctcatt gcgcttgcac caggggaatc aacaacttca
    26101 gagatgtcgt tgcgggttgc gttactgtaa tttataaaat gccggataag gcgttcacac
    26161 cgcatccggc actcggcgca cgatgcctga tgcgacgctt gccgcgtctt atcaggccta
    26221 caattggtgt tggaacggta ggcaggataa ggcgttcacg ccgcatccgg catctttcct
    26281 aacgccgatc gagagaaatc gcccccggcc cggtaattgc caacagcagg aatgcgccag
    26341 cgatactgac attcttccag aaattaatca tatttggccc aaccgcatcg ccggtcatat
    26401 cccagtaatg gtgaccaatc accgccgtac ccagcgtgta gaaaataaac agcaccgcca
    26461 gcggacgggt gaaaaagcca agcacgatta atatcgcggc gggcacttcc ataactaccg
    26521 caataatcgc tgccagcatc ggcatcggcg cgcccagcga ggccatatat tggaccgtac
    26581 cgtcaaagcc catcattttg ggaaaaccaa aaataatgaa aattaagacc acggcgatac
    26641 gggcaattaa taacaaaacg gggcgtgcag caccaaaatc aaaataacgt agtgagttca
    26701 taaccaatcc ctttgcaaga taatgtgatt taaacgtaat acacactctt gcacctcgcc
    26761 acgcccctgg cattgagata ttcgctaatt atcctggtta cagtgattta agcgcgatgt
    26821 cctcatctat cagcagtttc gcatcaaacg ttttgccgct ctggatccat ttgcggatta
    26881 agcgaatctc acgcccctga ttcagcgtta ccgcaccgat aagcacgccg ttttgcagat
    26941 taaaccaaat cgccttctga gtttccgggt tgccacgaca aatccagtca tcgccatgca
    27001 tatcgccaat aaactgtaag ttatcactgt actgatcgct ccagaaccac ggcggcggca
    27061 gtcgcggaag cggtagcccc aacattgcgg cagcggcaat ttgcgcctgg ttattggcgt
    27121 tttcccagct ttcgcagcgg tgtagtgcac cattatcaat acgggtgatt gccacatcgc
    27181 caccggcaaa gatcgcggga tcgcaggtgc ggcaagcttc atcaatgaca ataccattgg
    27241 cagtatcaag gttggcctcg cgagccagtt ggtcgttggc gctgatacca ataccgtaaa
    27301 tcaccacatc ggcccgaagc gtttcgccac tttgcagcgt cagttctact ttttcaccat
    27361 cgaccacatg ttcaatggca ttattgagca gaatgcgcac accagcctgc tgatggcgcg
    27421 gtaaaagata gcgttgcacg ggcggtggtg cattacggcc catgacgctt gccgccagtt
    27481 caatcactgt caccttacat ccacgctgcg tggcgctggc agccagttcc agaccaatag
    27541 ttccggcacc gacaatcacg actgaccgtt cgggctgcag aacttctcgc agtctggcgg
    27601 catcaccggc atggcgcagg gtaaagcagc gttctcccag tgcatcaagc aacggcagcg
    27661 gtcgagctgc cgcgccggtt gctataaaaa gctgatccca gtgccagctt tcgccgttag
    27721 ctaacactaa ctcttgtgtg tcgcggccca atgttttgat ggttacaccg gaatgcagat
    27781 gaacattgtt ttcctgccac cagtgagcgg gtaacacaga ctgcaactgt ggggaatctt
    27841 ccagcaacat ggatttcgag agcggggggc gttcataagg aagatgttgc tcatcggaaa
    27901 acagatgcag ctcaccgctg aacccttgct ggcgtagcaa ggccgcagcc attgccgccg
    27961 cttgcccgcc accgacaata atgatcgttt tttctttcat cgtgcttcct taaagatcca
    28021 gcccagccgc tacgtggcga atgccgcgaa tcgctaaacc cgcatcagcg ttgatcatca
    28081 caccgcttaa tgcgcgatta ttgcgccgcg atgccaacat cacatacgga cccgtaaaat
    28141 ccgccggttg cgggaaaaat tgcagcggta aaatggcggc aattttctcc ggcgtcagag
    28201 actgcattat cgaggtttca ctttgcccga gcgcctgtgg gccgcgcagg tcgctggcca
    28261 taccacacgg gccgacgcca ttcacccgca ctttcggtgc cagttcataa gccagttggc
    28321 gaataagtcc ggttgcggca tgtttactgg cggtgtacag cgggccaccg ccgccgggat
    28381 accaggcggc gtttgacagt gtgaaaatca tgctgccttc actggcgatt aacgccggag
    28441 cgcaggcttt tgcgcccagc aggtaaccga gtacgttgac attaaacagc tcgtggaagc
    28501 cggtttcgag cgtctctgcg ggagtgttaa ccagtgaggc attgtgatcc cagatgcccg
    28561 cattgccgat aaaacaatcc agcttgccag aacgagtcag gatctgatta accgcgcgtt
    28621 gataatcggc atagcaggtc acgttacctt ccaccgccag aatatgttcg ccaaatcgct
    28681 gacgcagact ggcgactttt gccgccgaca gttccagcgt ggcaacctgc gcgccttctt
    28741 cgataaatcg ctcggccagc gccaggccta atcccgatcc gccgccggta ataaagatgg
    28801 actcgttatg cagatcgctc atggctgcgc ctccggtaag tcgatgaaga tgtcaccacc
    28861 ttcaacgtgt actggataag tggttagcgg atcggtggcg ggcaggcata acgctttccc
    28921 cgttttcagg caaaaactgg cggcgtgcag cgggcactcc accgtggcgt catcttccag
    28981 atacccttct gacatcgacg cattaccatg gctgcaacga tcgttaatgg cataaaactc
    29041 gccgcctacg ttgaacaggg cgatgacggg cgaggtatcg atccggagag cctcaccctc
    29101 cggcacatcc gcaacgggac acgcataaat tcgattcatc agaacagtac actcaggtta
    29161 tgggaggaga tgaccgcttg atccaggacg atatcccgtt ccagcaagcg ccagttcccg
    29221 tcctccagac ggcgaacttt gtcgaaacgc gttccaacat agaatgtttc atcgcgctct
    29281 ttttgtgccc gataaagtag ataattcacc ctcacggcaa atacgtttgg gatgtcggtt
    29341 tcgcttacct ggcagttgct gattaagtga cgagtgcgtg acggcggttc ttctgcccag
    29401 gccatgccgg tttccagacg ggcgattcgc cgttccagct ggtctttggt gtcattaaaa
    29461 atccaggttg tcggcggttg aacgcctttg cggcggtcgc gcgtttgtgc gttaaccgtg
    29521 gtgcgcatgg tgtaacgaat ctcttcgtcg agctgtgcca gccagtcacg aaatttccag
    29581 tcatccagca gactggcttc gtgaaagaga aactggctaa tgcggtgatg taactctagt
    29641 gaaacttgcg cactcatttc atcacctcct gctggtaagc ggcggtttta tcgagcactt
    29701 cctgccagct ttcgctactc agaagatcgg cccagcgttg gtacattcca cgagcggccg
    29761 tttctgagaa gatatagtta gtaatgcctg gaatgccgtc gtcgcgacgc ttttcctgac
    29821 caagccccat ttccagacac agtttgctgt tgcgggcgcg gtggcctttc agcaatttct
    29881 ggatttcaca ccagttctcc gagtcatcct gctcgagaaa accagcagga ccaaaagcac
    29941 gagtggcgct gttttcaaaa gcggctttaa cttcatcgga ggcggctttg tcagtaatac
    30001 agaacgccca cacttcgact tgatcagggc cgcgcggatg ccagacgcgg agcgtggcag
    30061 tgccgttgag ccatgaaagc gtggggaaaa tattgttatg acccgccagg cgcagggcgc
    30121 gaacttcacc taaacgttgt tctgcttcgg cataggtttc gcggtaatag ctggaaaccg
    30181 cgccatcgac ccagacatta gcatccggtt tttcagtaaa gaagaaaccg ctaccgtgac
    30241 cgtcctgacc aaattgcagc gcatctttgg cggtttccca caccgggcgg gcggtttgtc
    30301 catcaccgag acgcttatcg ctgccatcat ctttcgcccc taatacctga acggcagaag
    30361 catggctgaa cagagcatga tattggtcac tggcgaactg ctctgccggg aatttccagt
    30421 tgcagttgat cacccacttt tgtacaccgc cgacaatttc ggtgccgcct tcgcgacgat
    30481 ccagcatgcc atccagatac caggcaatgt cacccaggta atcacgcagg cccggtgcgc
    30541 tggtatccca gttgccaaaa atcagccctt tataactctc cacacaagga acttcgttta
    30601 gtccccagtg ggatttacac aacccttgtg ggtaggcgcg aggttccagc ggtacatcga
    30661 tcaactcgcc gttaatgcca taagaccagc cgtgatacgg acaggtaaag gcgcgagtgt
    30721 taccgcaatc tgcataactc acacgcatgg cccggtggcg gcattggttg agaaacgcct
    30781 taatgctgcc gtctttctga cgcactacga caaccgcatc ttcccccatg taggtgttaa
    30841 agaaatcacc atgttttggg atctggcttt cgtgggcgag aaatagccag caacgaccga
    30901 aaatgcgctc aagctccagt tggtaaatat ccgggtcggt ataaatacgc ggagtgaccc
    30961 gaccgttttg ggtgtcaatc agttggtaaa tgttcaaatc tgagggtgtg gtcatatttt
    31021 ttaatcctga aagataagat gtattgacct tctatttttg tgccataagg atgttgctgg
    31081 ttggttgctg atatgtgaaa tagttaattt ctaccagctg agacgttttt tcgataacaa
    31141 ggggaaggtg atggaactac ggcatttacg ctatttcgtc gcagtggcgc aggcactgaa
    31201 ctttacccgt gcggcggaaa aactgcatac ctcacagcct tcgttaagca gccagatccg
    31261 cgatcttgaa aactgtgtcg gtgttccgtt gctggtgagg gataagcgca aagtcgcgtt
    31321 gacggcggcg ggagaatgtt ttctccagga cgcgctggca atcctcgaac aagcggaaaa
    31381 tgccaaatta cgggcgcgga aaattgttca ggaagacaga caattaacca ttggctttgt
    31441 gccatcggcg gaagtgaatt tactgccaaa agtattaccg atgtttcgtc tcagacagcc
    31501 agacacctta attgagctgg tgagtttaat caccacggaa caggaagaaa aaattcgccg
    31561 tggtgaactc gacgtcggct taatgcgcca tcctgtttat agcccggaga ttgattatct
    31621 ggagcttttt gacgaaccat tagtggtggt gttaccggtt gatcaccctt tagcacatga
    31681 aaaagagatc actgccgcgc aactggatgg cgtgaatttc gtcagtactg atccagcgta
    31741 ttccggttcg cttgcgccga tcgttaaagc gtggtttgcg caagaaaaca gccagccaaa
    31801 tatcgtccag gtggcaacga atattctggt gaccatgaat ctggtgggga tggggctggg
    31861 cgtcactttg atacccggtt atatgaataa ttttaatacc ggccaggtgg tatttcgacc
    31921 cattgccgga aatgtgcctg ccattgcttt actgatggcg tggaagaaag gggagatgaa
    31981 gccggcatta cgcgatttca tcgccattgt gcaggaacgt ttggcaagcg taacggcata
    32041 attacgcatt atttttgcaa aacgcgtcgc ggatcgcagt tttttagtgc ttgtgaactg
    32101 gcgttacaga catcgggaag caggctcgaa tattcttatt tttcaacttg caccaacatt
    32161 cgattaaccc cagactaacg cctcctgacg ggagggactc atggttttgc aatccacgcg
    32221 ctggttggcg ctcggctatt tcacatactt ttttagttac ggcatttttc tacctttctg
    32281 gagcgtttgg cttaagggga ttggtttaac gccagaaacc atcggcctgt tattgggggc
    32341 aggtctggtt gcccgttttc tcgggagctt gctcatcgcg ccccgcgtca gcgatccttc
    32401 tcgcctgatt tccgccttgc gcgtgctggc actgctgaca cttctctttg ctgtcgcctt
    32461 ctgggcgggg gcgcacgtag cgtggctgat gctggtgatg attggcttta acctcttttt
    32521 ctcaccgctg gtaccgttga ccgatgcact ggcgaatacg tggcaaaagc agttcccgct
    32581 tgattacggc aaagtgcgat tgtggggctc ggtggcgttt gtcattggct cgtcgctgac
    32641 gggcaaactg gtcactatgt ttgattatcg ggtgatcctc gcgctgttga cgttgggcgt
    32701 ggcatccatg ctgctcggct ttctcatccg tccgacgatt cagccacaag gggcaagccg
    32761 ccagcaggag agcaccggtt ggtctgcgtg gttggcgttg gttcgccaga actggcgctt
    32821 tctggcctgc gtttgtttat tgcagggggc gcatgcggcc tattacggtt ttagcgccat
    32881 ttactggcag gctgctggct actcggcctc ggcggtgggc tatttgtggt cgctgggcgt
    32941 ggtggcggaa gtcattatct ttgcgctgag taataaactt ttccgccgtt gtagtgcacg
    33001 cgatatgctg ttgatctcag cgatttgcgg cgtagtgcgc tggggcatta tgggagcaac
    33061 tacggcgttg ccgtggttga tagtggtgca aattctgcat tgtggcacct tcacggtctg
    33121 ccacctggcc gccatgcgct atattgccgc tcgccagggt agcgaagtca tccgtttaca
    33181 ggcggtttac tctgccgtcg cgatgggcgg cagtattgcg atcatgaccg ttttcgccgg
    33241 tttcctgtat caatatctgg gccacggcgt gttctgggtg atggcgctgg tggcgcttcc
    33301 ggcaatgttt ttgcgcccga aagttgttcc ctcatgctga ttcgagcatt ttgcggatct
    33361 gctcctgttg atgtgttgtc aacgtcaggt ccgtatagat cagcggtggt gaaaagagcg
    33421 gtaacggcat ggtataagga gcaataatca gtgccgcgct gcgcggagcg cctgtctgca
    33481 aaaatgcctt taccgacata tgtttaatat tgagcggcag taacgttagt tcgcgtagct
    33541 gctgctcaat ctgcgcttct cgctcgctat cattcccggt cagtagaata atctgtttct
    33601 catgcaggtc gttttcctgc attagccagg caccaaaaat caccgcgacc agaccgcttt
    33661 cctcatcaga aagatggacg ccgtattcac tttcaaatcc ggccagcgcc gcgcgggtgg
    33721 tgcgcaccag gcgtggatac aggcgggcga actcttccgg cagggtgtta tcaataccga
    33781 tggcaaacaa actgcgattt aacgcctggg cgaggtgggt ataaagctga tcgcataacc
    33841 cctgttcatc ataaaaacga acatttccca gctcacgaaa atggtttacc agacgtttga
    33901 tagacttacg cagttgtctg tcccgctgat gcgcatcccg cagtggatcg ggaacccgca
    33961 acatggaaaa aagtaatgcc ataaacagtg gctcatcagg cggtacaggc tggagcgccc
    34021 gacgctgcca gtggcgtcca atttcctgcg ctacctgaaa ttcaaggcag gattccgccc
    34081 agcgacgttg gagcggatta aactgaggcg taattccagc gtggtgttgc agcaaacaat
    34141 attgcagata cagacacagg aaatgaatat cgcgcgtttc aaagcgtttt tgcagccgtc
    34201 gggagcagag attcactaac gcttgtagat tggtatcgtc gtaaaagtta cgcgcaattc
    34261 cgcgccgctt cagttcactc ttcagggcgg gggtgaaatg gctggtaata aatgacggac
    34321 acagacggaa accacgtcgt agccagtgaa ataaacacaa acgttggtta agcactgtac
    34381 cttcaacccg gtagctaccg tcataaccag tagtcagtgt gagttgatga tagcgcagga
    34441 tctcccgtcc tgtttcgctg atatcaagac tggcaatatc atcatccaca ccattaagct
    34501 cgctgaaagt tgccgtggtg gcggtcaacc ccggctgaaa gagcgtcagc aagatctggc
    34561 agcggcgctg gggagccgaa aggacagatg gtggagcaag cgtaggcatc atctgtagat
    34621 ctcccataag cataattgat aagaatagct aaagtttgct cagggaaatg gcgaagggaa
    34681 tgctaatcat cagaaatgtt ggcaagcgtc acagaatttt tctcatctta aactgttctg
    34741 accagtggat tagcatgtct taaggtggtt ttgtgaatga ttatcgtgct agcgatcatg
    34801 ggttagttct gcggattcaa cgcgctaagg ataaaatcca ccatctgttc gcgataaaca
    34861 tcgccgtgac gatcggaaaa agcatcaatg tgttcgccat ccgggattag gatcaaccgt
    34921 tttggctctt ttgccaggct atacaacttt tcgctgtgct gccatgggat aacgtgatca
    34981 gctttaccgt gaatcagtaa aagcgggatc gggctgacgc tggcgatgta attttcgccg
    35041 ctgtaactct catcaagtaa gtagccactg ccggggatca tctggttggc gatggtcgca
    35101 taagaggcaa atgtggagtc gaggatcacc gcacgtatgc cttcacgatc accctgacca
    35161 ataacatcca aaatattcgc cccgccaata ctctgcccga acagcaccag ccgttgtggg
    35221 tttacatcac tgcgatggcg caccacattg atggcacttt gcgtatcgtc cagcaatccg
    35281 gcctgggacg gcgtgccttt tgatttacca aacccgcgat aatcaaacat aaaaacgttg
    35341 aaattacgct cgggtaacca actgaccagc ggccagtggg cggacatatt tccggcattg
    35401 ccgtgagcat gaataatggt ggcaatggcc ttttcagcgg ggccagttgc agaagggata
    35461 aaccaaccat gtaggcgagt gccatcttta gcggtgaatt ctactgattc ggctgaccag
    35521 ggatcggggc cgtagatttt gtcgtcaggg taatagaaaa aattgatggc gatgcgcggt
    35581 attaaataaa tgcagaaggc aacaacaaag agaataaaca gaattttgag aacgcgtttg
    35641 ttcactggca gcgccataac ctttccttgc gacttcttta tcatccatta atcggggaaa
    35701 gatggtggga gagtggcgaa agaaagtaaa gcggtgagga agtatttcgg aatagcgcct
    35761 gaaaggcgag ggcgggtgag tgacatcacc cgccagagtc attaacgctt cagggcgtcg
    35821 cttaactcgt cacgcatgtt cgccagcatg gctttaacaa cgcgcgggtt accagcaacg
    35881 atgttaccgg tcagcatgta gttatgacca ccggtgaagt cgctgacgat gccgcccgct
    35941 tcacgaacca gcagctcgcc tgccgcgaaa tcccacggac gcagaccgat ttcaaagaaa
    36001 ccgtcaacgc gacccgcagc aacgtaagcc agatccagcg ccgcagaacc ggtacgacgg
    36061 aagtctgcac attcgttgaa cagtttgccg acgatgttga tgtaggtagt ggcgtactgt
    36121 tttgctttga acgggaagcc ggtcgccaga atagtaccgt cgagatcgcg agcggtgctg
    36181 ccgcgcagac ggtagccgtt cagctgtgcg ccctgaccgc gagtggcggt gaacagttcg
    36241 ttacgcatag gatcgtaaac cacagcaact tcagtgcggc ctttgatacg aacggcgata
    36301 gataccgcga agtgcggcag acgtttgata aagttggtag tgccatccag tggatcgata
    36361 acccattgaa catcctgatc agtaccttca agttcaccgc tttcttcggt gatgatggtg
    36421 tgctgtgggt aagatttacg aatcgtgtcg ataatcaccg cttcggcagc tttatctacg
    36481 ttggtcacga agtcgttact gcctttctgg ctcgcttcta cagcgtccgg ggtttcatag
    36541 tttttggcaa ttaaattacc cgccttgcgc gctgcgcgca cggcgatgtt cagcatcgga
    36601 tgcatcggtc tctctcactg gatgttaaag aacgggaaaa cggcgcagag tatagcagcg
    36661 agatcggcat ctgtcttcgt tttatgataa gatgcgtgaa taatctttag atgaagaaag
    36721 acaatgctgc aaaatattcg aattgtgctg gtggagacgt cacacaccgg caatatgggg
    36781 tctgttgccc gtgccatgaa aacaatggga ttaaccaatc tgtggctggt taatccactg
    36841 gtgaaacctg actcccaggc gattgccctg gcagcagggg ccagcgatgt gattggtaat
    36901 gctcacatcg tcgatacgct cgacgaagcg ttagccggtt gtagcctggt ggtgggcacc
    36961 agcgcacgct cccgcacgct gccgtggccg atgctcgacc cgcgcgaatg cggtctgaaa
    37021 agcgtcgctg aagcggcaaa taccccggtg gcactggtgt ttggtcgcga gcgcgtcggc
    37081 ttgaccaatg aagagctgca gaaatgccat tatcatgtcg cgattgcggc taacccggaa
    37141 tacagttcgc tgaacctggc gatggcggtt caggttattg cctatgaagt gcgcatggcc
    37201 tggctggcaa ctcaggaaaa tggcgagcag gtcgagcatg aagagacgcc gtatccgctg
    37261 gtcgatgatc tggagcgttt ttatggtcat ctggaacaaa cgctgctggc aaccggtttt
    37321 attcgtgaaa accatccggg gcaggtgatg aataaattgc gccgtctgtt tacccgtgcg
    37381 cgcccggaaa gccaggaatt gaatatcctg cgcgggattc tggcttctat agagcagcag
    37441 aataaaggta acaaggccga ataatagtgg ttgcctgatg cgacgcttaa cgcgtcttat
    37501 caggcctgca gtgcacagag cccctgggcg gataaggcgt tcacgtcgca tccgacaaca
    37561 ggtacaaacg ccacgataaa aaaatggcac tgaaggttaa atacccgact aaatcagtca
    37621 agtaaatagt tgaccaattt actcgggaat gtcagacttg accctgctat gcaatacccc
    37681 cacttttaca ataaaaaacc ccgggcaggg gcgagtttga ggtgaagtaa gacatgagac
    37741 tgacatctaa agggcgctat gccgtgaccg caatgcttga cgttgcgctc aactctgaag
    37801 cgggcccggt accgttggct gatatttccg aacgtcaggg aatttccctt tcttatctgg
    37861 aacaactgtt ttcccgtctg cgtaaaaatg gtctggtttc cagcgtacgt ggaccaggcg
    37921 gtggttatct gttaggcaaa gatgccagca gcatcgccgt tggcgaagtg attagcgccg
    37981 ttgacgaatc tgtagatgcc acccgttgtc agggtaaagg cggctgccag ggcggcgata
    38041 aatgcctgac ccacgcgctg tggcgtgatt tgagcgaccg tcttaccggt tttctcaata
    38101 acattacttt aggcgaactg gttaataacc aggaagtgct ggatgtgtct ggtcgtcagc
    38161 atactcacga cgcgccacgc acccgcacac aagacgcgat cgacgttaag ttacgcgctt
    38221 aataaaaaga attcagaatc aggccggagt gctaaacact ccgtaaacac ggtcgtacat
    38281 ccagccggta gcctggtttc ttgcattgag tgatgtacgg agtttataga gcaatgaaat
    38341 taccgattta tctcgactac tccgcaacca cgccggtgga cccgcgtgtt gccgagaaaa
    38401 tgatgcagtt tatgacgatg gacggaacct ttggtaaccc ggcctcccgt tctcaccgtt
    38461 tcggctggca ggctgaagaa gcggttgata tcgcccgtaa tcagattgcc gatctggtcg
    38521 gcgctgaccc gcgtgaaatc gtctttacct ctggtgcaac cgaatctgac aacctggcga
    38581 tcaaaggtgc agccaacttt tatcagaaaa aaggcaagca catcatcacc agtaaaaccg
    38641 aacataaagc ggtactggat acctgccgtc agctggagcg cgaaggtttt gaagtcactt
    38701 acctggcacc gcagcgtaat ggcattatcg acctgaaaga actcgaagca gcgatgcgtg
    38761 acgacaccat ccttgtttcc atcatgcacg taaataacga aatcggcgtg gtgcaggata
    38821 tcgcggctat cggcgaaatg tgccgtgctc gtggcattat ctatcacgtt gatgcaaccc
    38881 agagcgtggg caaactgcct atcgatctga gccagttgaa agttgacctg atgtctttct
    38941 ccggtcacaa aatctatggc ccgaaaggta ttggtgcgct gtatgtgcgt cgtaaaccgc
    39001 gcgtacgtat cgaagcacaa atgcatggcg gcggtcacga gcgcggtatg cgttccggca
    39061 ctctgcctgt tcaccagatc gtcggtatgg gcgaagccta tcgcatcgca aaagaagaga
    39121 tggcgaccga gatggaacgt ctgcgcggcc tgcgtaaccg tctgtggaac ggcatcaaag
    39181 atatcgaaga agtttacctg aacggtgacc tggaacacgg tgcgccgaac attctcaacg
    39241 tcagcttcaa ctacgttgaa ggtgagtcgc tgattatggc gctgaaagac ctcgcggttt
    39301 cttcaggttc cgcctgtacg tcagcaagcc tcgaaccgtc ctacgtgctg cgcgcgctgg
    39361 ggctgaacga cgagctggca catagctcta tccgtttctc tttaggtcgt tttactactg
    39421 aagaagagat cgactacacc atcgagttag ttcgtaaatc catcggtcgt ctgcgtgacc
    39481 tttctccgct gtgggaaatg tacaagcagg gcgtggatct gaacagcatc gaatgggctc
    39541 atcattaatc ggtatcggaa tcaggagaat ttataatggc ttacagcgaa aaagttatcg
    39601 accattacga gaatccgcgt aacgtgggtt cctttgacaa caacgacgag aacgtcggca
    39661 gcggcatggt gggtgcaccg gcctgtggcg acgtgatgaa gttgcagatt aaagtcaacg
    39721 atgaaggtat cattgaagac gcgcgtttta aaacttacgg ctgcggttct gctatcgctt
    39781 ccagctccct ggtcaccgaa tgggtgaaag ggaagtctct cgacgaagcg caggcgatca
    39841 aaaacaccga tattgctgaa gaacttgaac tgccgccggt gaaaattcac tgttctattc
    39901 tggcagaaga cgcgatcaaa gccgccattg cggactataa aagcaaacgt gaagcaaaat
    39961 aagagttgag gtttggttat gtcgattaca ctgagcgaca gtgcagcagc gcgagtaaat
    40021 acctttctgg ctaaccgcgg caaagggttt ggcctgcgtc tgggcgtgag aacctccgga
    40081 tgttcaggta tggcttatgt actggaattt gttgacgaac cgacgccgga agacatcgtg
    40141 tttgaagaca aaggcgtgaa agtcgtggtc gatggcaaaa gcctgcaatt tctggacggt
    40201 acgcagctgg acttcgtaaa agaaggcctg aacgaagggt ttaaattcac caacccgaac
    40261 gtcaaagatg agtgtggttg cggcgaaagc ttccacgttt gatgcgcata ccgataaccc
    40321 caccgtggtc gcctgcgcgt ggggtttgtt ttacctgatt cgccgttatc gcggcggatc
    40381 gcagccctga gaatgttatg gattacttca ccctctttgg cttgcctgcc tgttatcaac
    40441 tcgataccca ggcgctgagc ctgcgttttc aggatctaca acgtcagtat catcctgata
    40501 aattcgccag cggaagccag gcggaacaac tcgccgccgt acagcaatct gcaaccatta
    40561 accaggcctg gcaaacgctg cgtcatccgt taatgcgcgc ggaatatttg ctttctttgc
    40621 acggttttga tcttgccagc gagcagcata ctgtgcgcga caccgcgttc ctgatggaac
    40681 agctggaact gcgcgaagaa ctggacgaga tcgaacaggc gaaagatgaa gcgcggctgg
    40741 aaagctttat caaacgtgtg aaaaagatgt ttgatacccg ccatcagttg atggttgaac
    40801 agttagacaa cgagacgtgg gacgcggcgg cggataccgt gcgtaagctg cgttttctcg
    40861 ataaactgcg aagcagtgcc gaacaactcg aagaaaaact gctcgatttt taatttctgg
    40921 aagctaaaca tggccttatt acaaattagt gaacctggtt tgagtgctgc gccgcatcag
    40981 cgtcgtctgg cggccggtat cgaccttggc acaaccaact cgctggtggc gacagtgcgc
    41041 agcggtcagg ccgaaacgtt agccgaccat gaaggccgtc acctgctgcc gtctgttgtt
    41101 cactatcaac agcaagggca ttcggtgggt tatgatgcgc gtactaacgc agcgctcgat
    41161 accgccaaca ccattagttc tgttaaacgc ctgatggggc gctcgctggc tgatatccag
    41221 caacgctatc cacatctgcc ttatcaattc caggccagcg aaaacggcct gccgatgatt
    41281 gaaacggcgg cggggctgct gaacccggtg cgcgtttctg cggacatcct caaagcactg
    41341 gcggcgcggg caactgaagc cctggcaggc gagctggatg gtgtagttat caccgttccg
    41401 gcgtactttg acgatgccca gcgtcagggc accaaagacg cggcgcgtct ggcggggttg
    41461 catgttctgc gcttacttaa cgaaccgacc gctgcggcta tcgcctacgg gctggattcc
    41521 ggtcaggaag gcgtgatcgc cgtttatgac ctcggcggcg ggacgtttga tatttccatt
    41581 ctgcgcttaa gtcgcggcgt gtttgaagtg ctggcgaccg gcggtgattc cgcgctcggc
    41641 ggcgatgatt tcgaccatct gctggcggat tacattcgcg agcaggcggg cattcctgat
    41701 cgtagcgata accgcgttca gcgtgaactg ctggatgccg ccattgcagc caaaatcgcg
    41761 ctgagcgatg cggactccgt gactgtgaat gttgcgggct ggcagggcga aatcagccgt
    41821 gaacaattca acgaactgat cgcgccactg gtaaaacgaa ccttgctggc ttgtcgtcgc
    41881 gcgctgaaag acgctggcgt agaagctgat gaagtgctgg aagtggtgat ggttggcggt
    41941 tctactcgcg tgccgctagt gcgtgaacga gtaggcgaat ttttcggtcg tccaccgctg
    42001 acttctatcg acccggataa agtcgtcgct attggcgcgg cgattcaggc ggatattctg
    42061 gtgggtaaca agccggacag cgaaatgctg ttgctggatg tgatcccgct gtcgctgggc
    42121 ctcgaaacga tgggcggcct ggtggagaaa gtgattccgc gtaataccac tattccggtg
    42181 gcgcgcgctc aggatttcac cacctttaaa gatggtcaga cggcgatgtc tatccatgta
    42241 atgcagggtg agcgcgaact ggtgcaggac tgccgctcgc tggcgcgttt tgcgctgcgt
    42301 ggtattccgg cgttaccggc tggcggcgcg catattcgcg tgacgttcca ggtcgatgcc
    42361 gacggtcttt tgagcgtgac ggcgatggag aaatccaccg gcgttgaggc atctattcag
    42421 gtcaaaccgt cctacggtct gaccgacagc gaaatcgcct cgatgatcaa agactcaatg
    42481 agctatgccg agcaggacgt aaaagcccgt atgctggcag aacaaaaagt agaagcggcg
    42541 cgtgtgctgg aaagtttgca cggcgcgctg gctgctgatg ccgcgctgtt aagcaccgca
    42601 gagcgtcagg tcattgacga tgctgccgct cacctgagtg aagtggcgca gggcgatgat
    42661 gttgacgcca tcgaacaagc gattaaaaac gtagacaaac aaacccagga tttcgccgct
    42721 cgccgcatgg accagtcggt tcgtcgtgcg ctgaaaggcc attccgtgga cgaggtttaa
    42781 tatgccaaag attgttattt tgcctcatca ggatctctgc cctgatggcg ctgttctgga
    42841 agcgaatagt ggtgaaacca ttctcgacgt tgcgctgcgt aacggtatcg agattgaaca
    42901 cgcctgtgaa aaatcctgtg cttgcaccac ctgccactgc atcgttcgcg aaggttttga
    42961 ctcgctgccg gaaagctcag agcaggaaga cgacatgctg gacaaagcct ggggactgga
    43021 gccggaaagc cgtttaagct gccaggcgcg cgttaccgac gaagatttag tggtcgaaat
    43081 cccgcgttac actatcaacc atgcgcgtga gcattaacag aggttagtat gggacttaag
    43141 tggaccgata gccgcgaaat tggcgaagca ctgtacgatg cgtatcccga tcttgatccg
    43201 aaaacggttc gattcaccga tatgcatcag tggatttgcg atctggaaga tttcgacgac
    43261 gacccgcagg catcgaacga gaaaatcctc gaagcgattt tgttggtctg gctggacgaa
    43321 gccgagtgaa tgaacgggct gcctgggggt agcccgtttt gctgttaaac gaataaggat
    43381 aactaaaatg acagaagcga tgaaaattac cctttctacc caacctgccg acgcgcgctg
    43441 gggagaaaaa gcgacttaca gcattaataa tgatggcatt accctgcatt tgaacgggac
    43501 agacgatctg gggctgatcc agcgtgcggc gcgcaagatt gacggtctgg gcatcaagca
    43561 tgttcagtta agcggtgaag actgggatgc ggatcgctgc tgggcattct ggcaaggtta
    43621 caaagccccg aaaggcacac gtaaagtgga gtggccggat ctggacgatg cccaacggca
    43681 ggaactggat aaccgcctga tgatcatcga ctgggtgcgt gacaccatca acgcaccggc
    43741 ggaagaactg gggccatcgc aactggcaca gcgtgctgtt gatctgatca gcaacgtcgc
    43801 gggcgatcgt gtgacttacc ggatcaccaa aggcgaagat ctgcgtgatc aaggttatat
    43861 ggggctgcac acggtcgggc gcggttcaga acgttccccg gtattgctgg cgctggatta
    43921 caacccaaca ggcgataaag aagcgccagt gtacgcgtgc ctggttggta aaggcatcac
    43981 ctttgattcc ggcggctaca gtatcaaaca gaccgcattt atggactcga tgaagtcgga
    44041 catgggcggc gcggcaacgg ttaccggagc gctggcgttt gccattacgc gcggactgaa
    44101 caagcgcgtg aagctgttcc tctgctgtgc ggataacctg atcagcggca atgcgttcaa
    44161 gctgggcgat atcattacct atcgcaacgg taaaaaagtt gaagtgatga acaccgatgc
    44221 ggaagggcgt ctggtgctgg ccgatggcct gattgatgcc agtgcgcaga aaccggaact
    44281 gatcattgat gcggcgaccc tcaccggggc ggcgaaaacc gcgctgggta atgattatca
    44341 cgcgctgttc agttttgacg atgcgcttgc cgctcgcttg ctggcgagtg ccgcgcagga
    44401 gaatgaacca ttctggcgtc tgccgctggc ggagttccac cgcagccagc tgccgtctaa
    44461 ctttgccgaa ctgaacaata ccggaagcgc ggcgtatccg gcaggtgcaa gcacggcggc
    44521 gggcttcctg tcgcactttg ttgagaacta tcagcaaggc tggctgcata tcgactgctc
    44581 ggcgacttac cgtaaagcgc cggttgaaca gtggtctgcg ggcgcgaccg ggctaggtgt
    44641 gcgcacgatt gctaatctgt taacggcgta aacgtcttat ttggcctaca aaattgcgaa
    44701 aatccaatag attgagattc ccttgtaggc ctgataagcg tagcgcatca ggcacttgtg
    44761 ccccgaacgt tgaatgaatt gtggttctta tgtccgaaac aaaaaacgaa cttgaagacc
    44821 tgctggaaaa agcagcaacc gaaccggcgc accgcccggc ctttttccgt actctactgg
    44881 aatccaccgt ctgggtgccg ggtacggcgg cgcagggcga ggctgtggtt gaagatagcg
    44941 cgcttgattt acagcactgg gaaaaagaag acggcaccag cgtcattcct ttttttacct
    45001 cgttagaagc acttcaacag gcggttgaag acgaacaggc atttgtcgta atgcccgttc
    45061 gcacattgtt tgagatgacc cttggcgaaa cgctcttcct taatgccaaa ctgccaaccg
    45121 gtaaagaatt tatgccgcgt gaaatcagtt tgctgattgg tgaagaagga aatccgctga
    45181 gcagccagga agtactggaa ggcggtgaat cgctgatatt atcggaagtc gcagagccac
    45241 cagcacaaat gattgattca ctcaccacct tatttaaaac tattaagccg gtgaagcgtg
    45301 cttttatttg ttcaattaaa gagaacgaag aggcacagcc taatttactt attggcattg
    45361 aagccgatgg tgatatcgaa gaaattattc aggcggcggg aagtgtagcg accgatacat
    45421 tacctggcga tgaacccatc gatatttgtc aggtgaaaaa aggggaaaaa ggaattagcc
    45481 actttattac cgaacatatt gcgccattct atgaacgtcg ctggggtggt tttttgcgtg
    45541 actttaaaca gaaccggata atctaaaata tgccgcccca aagggcggca ttcgggctta
    45601 cttgctgctc tgaaggttgt taaccgccag taaattgttg ccggagagac tccggttatt
    45661 atttacctcg cttaacagcg caccagaaac aataagtacg atacaaaagc accgtatctt
    45721 cattgtgcgg tccatgcgaa gaccgcatca cgacagcacc actggcgata ttgccgcgat
    45781 agcaagcgct gagacgcgct tgctctaacg gtcgcggaac atcctttcct taagccagcg
    45841 cccgcttgcg agacggcgga aaggtccgca gtggataata cactaacaga acaataataa
    45901 ctctctttta cttgacaaaa agagagttac tggcgagtat tgtgtccgct gagacgcgct
    45961 tgcattacta cggtaataca atctgctgga aaggccgcga accaaaccag caacaaataa
    46021 ccgcctgatt ttggcggttt tttgtttttt aaaggaaagt gaatttacat gtttaagtaa
    46081 tatcttttac cggttagtat ttttaatttt gagcaggatt tgtttatatt tgtctgaatt
    46141 cggactaata agccaggtta ttttattacc gaacacggcg gaaagggccg gtgtcgataa
    46201 tacgctaaat aaacaatgat tactctcttt cgcttgacaa aaagagagtt actggcgagt
    46261 attgctttgc tgtgaagcac ctgcgttgct catgcaacac aagccaatct gctggaaagg
    46321 ccgcgaacca gaccagcaat aaaaaaccgc cagattcggc ggttttttat tgcctgttaa
    46381 aagcttattt ctctggctca actggtaaat ctgctcgtgc gccccattca ctccatgcgc
    46441 cgtcgtacag tttcacgttt ggcacatcaa gtgtcgcgag tgccaacaaa accacggctg
    46501 ccgttacacc agagccgcag ctgacgataa taggtttgtc ataacttacg ccgcgaccaa
    46561 aaaatatcgc atccagttca tcggtcgttt ttagttcacc ttcgcgcacc agttccgtcc
    46621 acggaacatt tagtgcaccg ggaatatgtc cgcgacgtaa acctgggcga ggttcatcaa
    46681 cttctgcgtt aaaacgtgca gccgggcggg catcgataat ttgcgccgta ttttcatggc
    46741 ttgccaataa cacatcggtt actttcacca cggcttcagg attaaacgcg gcgttaaact
    46801 ctccttccgg cagctctact gcgccttctt ctaatagcag atcatcacgc tgccagcctg
    46861 caagtccacc acccagaatc gacactttct ctacaccaaa ggtgcgcagc atccaccatg
    46921 ctcgtggggc tgagaaaaga ttaccttcgt catagacaat caggtgctta tcctggttaa
    46981 cgcctaattc acgcatcgcc acggcgaacg tttccgggcg cggcagcatg tgcggaagcg
    47041 gggaagtgtg atcagaaagc gcttctatat caaaaaacac tgcgccggga atatgtccat
    47101 tcagatactc ctgagcaacg ttacgatctt cctgtccagg cgacgccatg cgggcatcga
    47161 taatctgaat ttccgggtca tcaatatgtt cggcgagcca gtcggctcct acaaaccatg
    47221 tcgtggacat aggcatctcc atttttgccg gggttaggct aaattgtcgg atgtttagtg
    47281 aaaggcgaca agcaaaagag gctgatttgg ctgtctgagc cacaattctc actaaaattc
    47341 ggattgtatg ggcgtgatac tgaattacat aacgtaaatt ctcacgcata ataattattc
    47401 gacgttgcta acgggccatt gtggcaggga tgaaaaatga aaaagttacg cgtagccgcc
    47461 tgcatgctaa tgctggcgct ggcagggtgc gacaacaacg ataacgcgcc aacagcggtg
    47521 aaaaaagatg cgccttctga agttactaaa gcggcctctt cagaaaacgt gagttcagca
    47581 aaactttccg cgccagagcg acaaaaactg gcccaacaga gtgccggtaa ggcgctgaca
    47641 ttgctggatc tctctgaagt tcaacttgat ggcgcagcca cgctggtgct gacattctcc
    47701 atccctctcg acccggatca ggaattctca cgcgttatcc atgtcgtcga taagaaaagc
    47761 ggcaaagtgg atggtgcctg ggagctgtca gataatctta aagagttgcg tttacgccac
    47821 ctcgaaccga aacgtgattt gatcgtcact attggcaagg aggtcaaagc actcaacaac
    47881 gcaaccttca gtaaagatta cgaaaaaact ataactaccc gagatatcca acccagcgtc
    47941 ggttttgcca gccgtggctc gttgttacct ggtaaagtta ttgaagggct gccggtaatg
    48001 gcgctcaacg ttaacaatgt cgatgttaac ttcttccgtg ttaagccaga atctctgcct
    48061 gcattcatta gccaatggga ataccgcaat tcgctggcga actggcagtc tgacaaactg
    48121 ctgcagatgg cggatctggt ttataccgga cggtttgatc tcaattctgc gcgtaacacc
    48181 cgtgaaaaat tattgctgcc gctgggcgat atcaaaccgc ttcagcaggc aggcgtgtat
    48241 ctggctgtga tgaatcaggc tggacgttac gattacagta atcccgcgac gctgtttacg
    48301 ttaagtgata tcggcgtttc agctcaccgt tatcacaatc gtctggatat ctttacccaa
    48361 agcctggaaa acggcgcggc ccagcaagga gttgaagtct cattattaaa tgagaaaggg
    48421 cagactctga ctcaggcaac cagtgatgcc caggggcatg tgcagctgga aaatgataaa
    48481 aacgcggcat tactgttggc gcgtaaagac ggtcagacaa cgctactcga tttaaaactt
    48541 ccggcgctgg acttagcaga atttaacatt gctggcgcgc caggctatag caaacagttt
    48601 ttcatgtttg gcccgcgcga tctttatcgc cctggtgaaa cagtcatcct caatggttta
    48661 ttgcgtgatg cagatggtaa agcattgccc gatcaaccca tcaagttaga cgtgattaaa
    48721 cccgacgggc aggtactcag gagcgtcgtt agtcagccgg agaatggcct ctaccacttt
    48781 acctggccac tcgatagcaa tgcggcaacc ggtatgtggc atattcgcgc taacacgggc
    48841 gataaccagt atcggatgtg ggatttccac gtcgaagatt ttatgccaga gcgcatggcg
    48901 ctgaatctga ccggtgagaa aaccccgcta acgccgaaag atgaagtgag attctccgtg
    48961 gtggggtact acctgtatgg tgcgcctgct aatggtaata ctttgcaagg gcaacttttc
    49021 ctgcgcccac tgcgtgaagc tgtgtcagcc ttacctggtt tcgaattcgg cgatatagct
    49081 gccgaaaatc tttcccgcac gctggatgaa gttcagttga cgctggatga taaagggcgt
    49141 ggcgaagttt ctacagaaag ccagtggaag gaaacgcatt ccccattaca ggttattttc
    49201 cagggtagtt tgttggaatc gggcggtcgc ccggtgacgc gccgcgctga gcaggctatc
    49261 tggcctgccg atgcattgcc ggggatccgt ccgcagttcg cctcgaaatc ggtttacgat
    49321 tatcgcactg acagcacggt gaaacagccc attgttgatg aaggcagtaa cgccgctttt
    49381 gacatcgttt atagcgatgc gcaaggcgta aaaaaagccg tgtcgggctt gcaggtgcgc
    49441 ctgattcgcg aacgccgcga ttactactgg aactggtcag aagatgaagg ctggcagtca
    49501 cagtttgatc aaaaagatct gatcgaaaat gaacaaactc tggatctgaa agcggacgaa
    49561 accggcaagg tcagtttccc ggtagagtgg ggcgcttatc gtctggaagt caaagcgccg
    49621 aatgaagcgg tcagtagtgt gcgtttctgg gctggctata gctggcagga caacagcgac
    49681 gggagcggtg ccgtgcgacc cgaccgtgtc acgctgaaac tggataaagc cagctatcgc
    49741 cctggcgata ccattaagtt gcatattgcc gcgccaacgg cgggtaaagg ttatgcgatg
    49801 gtcgagtcca gtgaagggcc gctgtggtgg caagagattg atgttccggc tcaagggctg
    49861 gatctgacga ttccggtcga taaaacctgg aatcgtcatg atctgtattt aagtacgctg
    49921 gtggtacgtc ctggcgataa atctcgctcc gcgacgccaa aacgcgcggt tggggtgttg
    49981 catctgccgc ttggtgatga aaaccgtcgc ctcgatctgg cgctggaaac ccctaccaaa
    50041 atgcgcccca atcagccatt aaccgtgaaa attaaagcca gcaataaaaa tggtgagatg
    50101 ccaaaacagg tgaatgtgct ggtgtctgcc gttgatagcg gtgtgctgaa tattactgat
    50161 tacgtcacgc cagatccgtg gcaggcgttc tttggtcaga aacgctatgg cgcagacatt
    50221 tacgatattt acggtcaggt cattgaaggt caggggcgtc tggcagctct gcgttttggt
    50281 ggtgatggtg atgagctgaa acgtggtggt aaaccgccgg tcaatcacgt caatattgtc
    50341 gcgcagcagg cgctgccggt aacgctcaac gaacagggcg aaggctcggt tacactgcca
    50401 attggcgatt ttaacggtga attgcgcgtc atggcgcaag cctggacggc agatgacttc
    50461 ggaagcaacg aaagcaaagt gattgttgct gcaccggtga ttgctgaact gaacatgcca
    50521 cgctttatgg cgagtggcga tacctcgcgc ctgacgctgg atatcactaa tcttaccgat
    50581 aaaccgcaaa aacttaatgt tgccctgacc gccagtggtt tgcttgaact ggtcagcgat
    50641 tcacccgcag ccgttgaatt agcgccaggg gtgcgtacta cgctgtttat cccggtgcgt
    50701 gcattgccgg gttatggcga tggagacatt caggccacca ttagcgggtt agcgttaccc
    50761 ggggaaaccg ttgccgatca gcataagcag tggaaaatcg gcgtccgtcc ggcattcccg
    50821 gcacaaacgg tcaattacgg tacggcgtta cagcctggtg aaacctgggc gctcccggct
    50881 gatggattgc aaaacttctc gcctgttacg ctggaagggc aattgttgtt gagcggcaaa
    50941 ccaccgctga acatcgcacg ttatatcaaa gagttaaaag cgtatccgta cggctgtctc
    51001 gagcaaacca ccagcggcct gttcccgtca ctttatacca atgcagccca actgcaggcg
    51061 ttgggcatca aaggcgacag tgatgagaaa cgccgtgcat cggtcgatat cggcatttcc
    51121 cgtttgctgc aaatgcaacg tgataacggc ggctttacgc tgtgggataa aaacggtgac
    51181 gaagagtact ggctgacggc ttacgtgatg gatttcctgg tccgcgcagg cgaacagggt
    51241 tacagcgtac cgacagacgc cattaaccgg ggtaatgagc gtctgctgcg ctatttacaa
    51301 gatccgggca tgatgtcgat cccgtacgct gataatctca aagccagtaa attcgccgta
    51361 cagtcttacg ctgcgctggt gttggcccgt cagcaaaaag caccactggg tgcgctgcgt
    51421 gaaatctggg agcatcgtgc agatgccgct tctggtttac cgctgctgca acttggcgtt
    51481 gcgctgaaaa ccatgggtga tgcaatgcgt ggtgaagaag cgattgtgct ggcgctgaaa
    51541 acgccgcgta atagtgatga gcggatatgg ctgggtgatt acggtagtcc actgcgcgac
    51601 agcgcgttga tgctctcatt gctggaagag aacaaactgc tacccgatga gcagtactcc
    51661 ctgttgaaca cactttcgca gcaggcgttc ggtgaacgct ggctatcgac gcaggaaagt
    51721 aacgcgttgt tcctggctgc ccgtactctt caggatttac cgggcaaatg gcaggcgcaa
    51781 accactttct ctgctgagcc gctgacaggc gagaaagcgc aaaccagcaa tctgaatagc
    51841 gatcaacttg ccaccttgca ggtgaccaac agtggcgatc agccgttatg gctgcgtgtg
    51901 gatgccagcg gttatccgca atccgcacct ttatcggcga gcaatgtgct gcaaattgag
    51961 cgtcatattc tgggtactga cggtaagagc aaatcgctgg actcgttacg tagcggcgat
    52021 ctggtgctgg tgtggttgca ggttaaagcc agtaacagtg taccggatgc gttagtcgtg
    52081 gatctgttgc ctgcggggct ggaactggaa aaccagaatc tggcgaacgg cagcgccagc
    52141 ctggagcaaa gtggtggtga agtgcagaac ttgctgaacc agatgcagca ggcgagcatt
    52201 aagcacatag agttccgtga cgatcgcttt gtggcggcgg ttgccgttga tgaatatcaa
    52261 ccggtaacgc tggtgtatct ggcgcgggcg gtgacgccgg gaacgtatca ggtgccgcag
    52321 cctatggtgg aatcaatgta tgttcctcaa tggcgggcga ctggtgcggc tgacgatctg
    52381 ttgatagtca gaccgtaaat gcctcgcctg ttaaccaaac gcggctgctg gataatgctg
    52441 gcagccgcgc cttttatcat cattctggca gcatgggcag cagataaact ctggcctctg
    52501 ccattgcaag aagtgaatcc tgcacgcgtg gtggtggcgc aggatggtac gccactctgg
    52561 cgtttcgccg atgctgacgg tatctggcgt tatccggtaa caatcgaaga tgtttctcca
    52621 cgttaccttg aagcgctgat caattatgaa gatcgctggt tctggatgca tccgggggtg
    52681 aatccgttct cggtggcgcg tgcagcctgg caagatctca cgtcgggtcg ggtcatttcc
    52741 ggcggcagca cgctcaccat gcaggtggcg cgcctgcttg atcctcaccc caaaacgttt
    52801 ggcggcaaaa ttcgccagct ctggcgcgcg ttgcaactgg aatggcatct gtctaaacgt
    52861 gaaattctga ccttgtatct taaccgtgcc ccgtttggcg gtacgttgca ggggattggt
    52921 gcggcaagtt gggcttacct cgggaaatcg cctgcgaatt taagctattc cgaggcggca
    52981 atgctggcgg ttttgccgca agcgcccagc cgtcttcgcc cggatcgttg gccggagcgt
    53041 gccgaagccg cgcgtaataa agtgctcgaa cggatggccg tgcaaggtgt gtggtcccgt
    53101 gagcgggtaa aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa
    53161 ctggcactgc tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact
    53221 acgctggatg ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaaggacga
    53281 ttgccaccgc gcagctcact ggcgatgatc gtggttgatc ataccgatat gcgtgttcgc
    53341 ggctgggtgg gatcagttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc
    53401 aatgcgatcc gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat
    53461 gaaggcttga tccacccggc atcgctgttg caagacgtcc ccagacgcac cggtgattac
    53521 cgacctggca actttgacag cggttttcat ggcccgatca gcatgagtga ggcgctggtg
    53581 cgatcgctga acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg
    53641 gcaaagttac gcaatgttgg attgccgtta tatttgccca acggtgctgc accgaatctt
    53701 tcgctcattc tcggcggcgc tggcgcaaaa ctggaagata tggcggcagc gtataccgcg
    53761 tttgctcgcc acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa
    53821 cggcctttaa tgtcgtcggg ggctgcgtgg atcattcgcc ggattatggc tgatgaagcg
    53881 caacccttgc cggatggtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc
    53941 agctatggct atcgtgatgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc
    54001 tggactggca gaccggacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccata
    54061 ccgctgttga atcaggttaa taatatctta ctgtcgcgca gtgtgaattt gccggaagac
    54121 ccacgtcctg actctgtaag tcgtggcgtt atctgctggc cgggcggaca gtctttggcg
    54181 gaaggtgacg gtaactgtcg ccgccgcctg gcaacctggc tgctggacgg gagccagccg
    54241 ccaactctat tactgccgga gcaggaaggc attaatggca ttcgtttccc catctggctg
    54301 gatgaaaacg gcaaacgtgt tgccgctgat tgccctcagg cgcgacaaga aatgataaat
    54361 gtctggccgc taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgcttg
    54421 ccaccggcct cgacaatttg cccgccatac ggtcacgatg cacaactccc gctgcagcta
    54481 acaggtgtgc gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg
    54541 ccgttgcaat caagtggagg ggcaggtgaa cgctggtggt ttctgaacgg cgaaccgtta
    54601 actgaacgtg ggcgcaacgt gaccctgcat ttgacggata aaggcgatta tcaattgctg
    54661 gtgatggatg aggtggggca aatagcgaca gtgaaatttg tcatgcaata gtctgttttg
    54721 tccctgattg ttgcttaaac tgggtttatt ttaaaaaaat gttaccttca tcaatagtca
    54781 acggctctgt tgctcattat aatccgcgcc atctcgtacg ctggtacaga caacaacaga
    54841 acaatttaca gaggtaaaaa tggctattga acgtactttt tccatcatca aaccgaacgc
    54901 ggtagcaaaa aacgtgattg gtagtatctt tgcgcgcttt gaagctgcag ggttcaaaat
    54961 tgtcggcacc aaaatgctgc acctgaccgt tgaacaggct cgtggctttt atgctgaaca
    55021 cgatggaaaa ccgttctttg atggtctggt tgaattcatg acttctggcc cgatcgttgt
    55081 ttccgtgctg gaaggtgaaa atgctgttca gcgtcaccgc gatctgctgg gcgcgaccaa
    55141 tccggcaaac gcactggccg gtactctgcg tgctgattac gctgacagcc tgaccgaaaa
    55201 tggtacccac ggttctgatt ccgtcgaatc tgccgctcgc gaaatcgctt atttcttcgg
    55261 cgaaggcgaa gtgtgcccgc gcacccgtta ataatttcgt aaatgccgcg tgcaaacgtg
    55321 gcatccgtgc gccagaattt gtacaatgca gcgcccccgg acgagcagcc gctcaccggg
    55381 gcgtttcttt tttcaaccct ccaggggcca taacgtgtaa taacgaggcc ggaatagcat
    55441 atgtctgaac aattagtcac acctgaaaac gtcaccacta aagatggaaa aatcaacctg
    55501 ctggatctca accgtcagca gatgcgggag ttttttaaag atttaggtga aaaacccttc
    55561 cgcgccgatc aggtgatgaa gtggatgtat cactattgct gcgacaactt tgatgagatg
    55621 accgacatca acaaagtgtt gcgcggcaaa ctgaaagagg tggcggagat ccgcgcaccg
    55681 gaagtggttg aagaacagcg ttcatctgac ggcaccatta aatgggcaat tgccgttggc
    55741 gatcagcgcg tcgaaacggt gtatatcccg gaagacgacc gtgccacgct ctgcgtctct
    55801 tcgcaggtgg ggtgtgcgct ggagtgtaaa ttctgttcca ccgcccagca gggctttaac
    55861 cgcaacctgc gggtatcgga aattatcggc caggtgtggc gtgcggcgaa aattgtcggc
    55921 gcagcaaaag tcaccggtca gcgcccaatc actaacgtcg tgatgatggg tatgggcgag
    55981 ccgctgctca acctgaacaa cgtcgttccg gcgatggaaa tcatgcttga tgatttcggt
    56041 tttggcctgt caaaacgtcg cgtcacgctt tccacttccg gtgtggttcc ggcgctggat
    56101 aaactgggcg atatgatcga cgttgcgctg gctatctccc tgcacgcgcc gaacgacgaa
    56161 attcgtgacg aaattgtgcc gatcaacaaa aagtacaata tcgaaacgtt ccttgcggcg
    56221 gtgcgccgtt atctggagaa atccaacgcc aatcagggcc gagtcactat tgaatacgtg
    56281 atgcttgacc acgttaacga cggcactgaa cacgcgcacc aactggcgga actgctgaaa
    56341 gatacgccgt gtaagatcaa cctgatcccg tggaacccgt tcccgggcgc gccgtatgga
    56401 cgcagttcga acagccgtat cgaccgtttc tcaaaagtgc tgatgagcta cggtttcacc
    56461 actattgtgc gtaaaacgcg tggtgatgac attgatgctg cctgtggtca gttggcgggc
    56521 gatgttatcg accgtacgaa acgtaccctg cgtaaacgaa tgcagggtga agccatcgac
    56581 attaaagcgg tctgatagcc attacgccac ggttacttta tgtaccgtgg cgtaatcttt
    56641 tatgaatgaa gtattaagtt gcaattgtct ggtcaatatt aacggtgcgt ttttgctgac
    56701 tttaaggcag tatgtaacga tgcaacagta agttagcctt agcgaatgtg ggctttgtca
    56761 cgagcacaca gacggtctta tactgtatga taacggttaa cttaacggat gtttcgcggt
    56821 gtgggtgggc atgatggttc accggcatct caattctcat ttaaacgtac ctgcagcgaa
    56881 tgaatactga agccacgcac gaccaaaatg aagcacttac taccggcgct cgcctgcgta
    56941 atgctcgcga acaactagga cttagtcaac aggccgttgc cgagcgactt tgcctgaagg
    57001 tttccacggt acgcgacatt gaagaagata aggcacccgc cgatcttgct tcaacattcc
    57061 tgcgcggata tatccgctct tatgcgcgtc tggtacatat tccagaagaa gaactgctgc
    57121 cagggctgga aaagcaggct ccactacggg ctgcaaaagt tgcgccgatg cagagttttt
    57181 ccctcggtaa acgccgcaaa aaacgtgacg gctggctgat gaccttcacc tggctggtat
    57241 tgtttgtggt tattggcctg agcggtgcct ggtggtggca agaccacaaa gctcagcagg
    57301 aagagatcac cactatggcc gatcaatctt ctgcggaact gaataataat caaagccaga
    57361 gtgtaccgtt agatacgtcg acaaccacag atcaggctat ggcgaccacg ccgacatcac
    57421 ctgtggatac caccgcaacc aacacgcaaa cacctgccgt aactgcgcca gcaccagctg
    57481 ttgatccgca acagaatgcg gttgttccac cttcgcaggc aaatgttgat accgctgcga
    57541 ctccggcacc agcggcaaca acaacaccag atggcgctgc gccgttgcca accgatcagg
    57601 ctggcgtgac cacgccggcg gttgacccga atgcgctggt gatgaacttt actgccgatt
    57661 gctggctgga agtcaccgat gctaccggta aaaaactgtt tagcggtatg cagcgtaaag
    57721 acggtaattt gaacttaacc ggccaggcac cgtacaaact gaaaattggt gcgccagccg
    57781 cagtacagat ccagtatcaa gggaaacctg tcgatctgag tcgttttatc agaactaacc
    57841 aggttgcgcg tctgaccctc aatgccgaac aatcaccggc gcagtaacag acgggtaacg
    57901 cgggagattt ttcatgcata accaggctcc aattcaacgt agaaaatcaa cacgtattta
    57961 cgttgggaat gtgccgattg gcgatggtgc tcccatcgcc gtacagtcca tgaccaatac
    58021 gcgcacgaca gacgtcgaag caacggtcaa tcaaatcaag gcgctggaac gtgttggcgc
    58081 tgatatcgtc cgtgtctccg taccgacgat ggacgcggca gaagcgttca aactcatcaa
    58141 acagcaggtt aacgtgccgc tggtggctga catccacttc gactatcgca ttgcgctgaa
    58201 agtagcggaa tacggcgtcg attgtctgcg tattaaccct ggcaatatcg gtaatgaaga
    58261 gcgtattcgc atggtggttg actgtgcgcg cgataaaaac attccgatcc gtattggcgt
    58321 taatgccgga tcgttggaaa aagatctgca agaaaagtat ggcgaaccga cgccgcaggc
    58381 gttgctggaa tctgccatgc gtcatgttga tcatctcgat cgcctgaact tcgatcagtt
    58441 caaagtcagc gtgaaagcgt ctgacgtctt cctcgctgtt gagtcttatc gtttgctggc
    58501 aaaacaaatc gatcagccgc tgcatctggg gatcaccgaa gccggtggtg cgcgtagcgg
    58561 ggcagtaaaa tccgctattg gtttaggtct gctgctgtct gaaggcatcg gcgacacgct
    58621 gcgcgtatcg ctggcggccg atccggtcga agagatcaaa gtcggtttcg atattttgaa
    58681 atcgctgcgt atccgttcgc gcgggatcaa cttcatcgcc tgcccgacat gttcgcgtca
    58741 ggaatttgat gttatcggta cggttaacgc gctggagcaa cgcctggaag atatcatcac
    58801 tccgatggac gtttcgatta tcggctgcgt ggtgaatggc ccaggtgagg cgctggtttc
    58861 tacactcggc gtcactggcg gcaacaagaa aagcggcctc tatgaagatg gcgtgcgcaa
    58921 agaccgtctg gacaacaacg atatgatcga tcagctggaa gcgcgcattc gtgcgaaagc
    58981 cagtcagctg gacgaagcgc gtcgaattga cgttcagcag gttgaaaaat aataacgtga
    59041 tgggaagcgc ctcgcttccc gtgtatgatt gaacccgcat ggctcccgaa acattgaggg
    59101 aagcgttgag ggttcatttt tatattcaga aagagaataa acgtggcaaa aaacattcaa
    59161 gccattcgcg gcatgaacga ttacctgcct ggcgaaacgg ccatctggca gcgcattgaa
    59221 ggcacactga aaaacgtgct cggcagctac ggttacagtg aaatccgctt gccgattgta
    59281 gagcagaccc cgctattcaa acgtgcgatt ggtgaagtca ccgacgtggt tgaaaaagag
    59341 atgtacacct ttgaggatcg caatggcgac agcctgactc tgcgccctga agggacggcg
    59401 ggctgtgtac gcgccggcat cgagcatggt cttctgtaca atcaggaaca gcgtctgtgg
    59461 tatatcgggc ctatgttccg tcacgagcgt ccgcagaaag ggcgttatcg tcagttccat
    59521 cagttgggct gcgaagtttt cggtctgcaa ggtccggata tcgacgccga actgattatg
    59581 ctcactgccc gctggtggcg cacgctgggt atttccgagc acgtaactct tgagctgaac
    59641 tctatcggtt cgctggaagc tcgtgcgaat taccgcgatg cgctggtggc attccttgag
    59701 cagcataaag aaaagctgga cgaagactgc aaacgccgca tgtacactaa cccgttgcgc
    59761 gtactcgatt caaaaaatcc ggaagtgcag gtgcttctca acgacgctcc ggcattaggc
    59821 gattatctgg acgaggaatc tcgtgagcac tttgccggtc tgtgcaaact gcttgagagc
    59881 gcgggtatcg cttacaccgt aaaccagcgt ctggtgcgcg gtctggatta ctataaccgt
    59941 accgttttcg agtgggtgac taacagcctc ggttcccagg gcaccgtgtg tgcaggtggt
    60001 cgttatgacg gtctggtgga acaactgggt ggtcgtgcaa caccggcagt cggttttgcg
    60061 atgggcctcg aacgtcttgt attgttagtt caggccgtta atccggaatt taaagccgat
    60121 cctgttgtcg atatatacct ggtggcttca ggtgctgaca cacaatctgc ggctatggca
    60181 ttagcagagc gtctgcgtga tgaattaccg ggcgtgaaac tgatgaccaa ccacggcggc
    60241 ggcaacttta agaaacagtt tgcccgtgct gataaatggg gtgcccgcgt tgctgtggtg
    60301 ctgggtgagt ctgaagtggc taacggcaca gcagtagtga aggatttgcg ctctggtgag
    60361 caaactgcag ttgcgcagga tagcgtagcc gcgcatttgc gcacgttact gggttaagga
    60421 aggagaagga cagcgtggaa atttacgaga acgaaaacga ccaggttgaa gcggttaaac
    60481 gcttttttgc tgaaaatggc aaagcactgg ctgttggggt gattttgggc gttggcgcac
    60541 tgattggctg gcgctactgg aatagccatc aggttgattc tgcacgctcc gcttctcttg
    60601 cctatcaaaa tgcggttacc gcagtgagcg aaggcaaacc ggatagcatc ccagcggcgg
    60661 aaaaatttgc tgctgaaaat aaaaatactt atggtgcgct ggcttctttg gaacttgcgc
    60721 agcaatttgt tgacaaaaat gaactagaga aagctgccgc ccagttacaa caggggctgg
    60781 cagacacgag cgatgaaaat ctcaaagccg tgataaatct gcgtcttgct cgcgttcagg
    60841 tacagctcaa gcaggctgat gccgcgctaa aaacccttga tgctatcaaa ggtgaagggt
    60901 gggctgccat tgttgccgac ctgcgtggtg aagcattgct gagcaaaggt gataagcaag
    60961 gtgcgcgtag tgcatgggaa gcaggcgtga aaagcgatgt taccccggca ctgagcgaaa
    61021 tgatgcagat gaaaattaat aatttgtcca tctgagaggg acccgatgca attgcgtaaa
    61081 ttactgctgc caggactgct ttccgttacc cttttaagcg gctgttcgct gtttaacagc
    61141 gaagaagatg tggtaaagat gtccccattg ccaaccgttg aaaaccagtt tacgccgact
    61201 actgcgtgga gcacttccgt tggtagcggc attggcaact tctattccaa tcttcatccg
    61261 gcactggcgg acaacgttgt ctatgcagcg gaccgcgctg gtttagtaaa agcgctgaat
    61321 gcggatgacg gcaaagaaat ctggtctgtc aacctggccg agaaagatgg ctggttctct
    61381 aaagatcctg cattactttc tggcggcgtg accgtgtctg gtgggcatgt ctacattggc
    61441 agcgaaaagg cgcaggttta cgcgctgaat accagcgatg gtactgtggc atggcaaact
    61501 aaagtcgcgg gtgaagcact ttcgcgcccg gtggtcagcg acggtctggt gttaatccac
    61561 accagtaacg gtcagttaca agcgctgaac gaagctgacg gcgctgtcaa atggacagtt
    61621 aacctcgata tgccgtcgct ctctttgcgt ggcgagtctg cgccggcaac ggcttttggt
    61681 gcggccgtcg tgggtggcga taatggtcgc gtcagcgcag tgctgatgga acagggccag
    61741 atgatttggc agcagcgtat ttcccaggcg accggttcta ccgaaattga ccgtctgagc
    61801 gatgttgaca cgactcccgt cgttgttaac ggcgttgttt tcgcgctggc ctataatggt
    61861 aacctgacgg cgcttgatct gcgcagcggt cagattatgt ggaaacgcga actgggttcg
    61921 gtgaatgatt tcatcgtcga cggcaaccgc atctatctgg tcgatcaaaa tgaccgggtg
    61981 atggcgttga ccattgatgg cggcgttacg ctgtggacac aaagcgatct gctgcatcgc
    62041 ctgctgactt ctccggtgct gtataatggc aacctggtgg taggtgacag tgaaggttat
    62101 ctgcactgga ttaacgtcga agatggtcgt ttcgttgccc agcaaaaagt tgatagttcc
    62161 ggtttccaga ctgaaccggt tgctgctgac ggcaaactgc tgatccaggc aaaagacgga
    62221 accgtgtact ctattacacg ttaatcgtct ctgtcgttca ctttgaaaac ggctcctgga
    62281 caggggccgt tttcctgttt ttaacaacga cacgaatata gcgtgcgttg tctgatgatt
    62341 tataaaaatg aggctttaaa catggtacct gtggtcgcgc ttgtcgggcg ccctaacgta
    62401 ggaaaatcca cgttatttaa ccgtctaact cgcacccgag atgcgctggt tgcggatttc
    62461 ccgggtctga ctcgtgaccg taagtacggt cgtgcggaaa ttgaaggccg cgagtttatc
    62521 tgcattgata ccggcgggat tgatggcacc gaagacggtg tagagacacg tatggcggaa
    62581 cagtcgctgc tggcgattga agaagcggac gttgtactgt ttatggtgga tgcgcgcgcg
    62641 ggcctgatgc cggcggatga agcgattgct aaacatctgc gctcccgtga aaaaccgacc
    62701 ttcctggtgg caaacaaaac tgacggtctg gatcccgatc aggcagtggt tgatttctac
    62761 gcgcttggtt taggtgaaat ctacccgatc gccgcgtctc acggtcgtgg cgtattaagt
    62821 ctgctggagc acgtgctgct gccgtggatg gaagatctcg caccgcaaga agaagtcgac
    62881 gaagacgccg aatactgggc gcaatttgaa gcggaagaga acggcgaaga agaagaggca
    62941 gacgacttcg acccgcaaag tctgccgatc aaactggcga ttgtgggtcg tccgaacgta
    63001 ggtaagtcta cactcactaa ccgtattctt ggtgaagagc gcgttgttgt ttacgacatg
    63061 cctggcacga cgcgtgacag tatctacatt ccaatggaac gcgacggacg tgagtatgtg
    63121 ctcattgaca ccgctggtgt acgtaaacgc ggcaaaatca ccgatgcggt agagaaattc
    63181 tcggtaatca aaacgttgca ggccattgaa gatgccaacg tggtgatgtt ggtgattgat
    63241 gcgcgtgaag gtatttccga tcaggatctc tccttgctgg gctttattct caatagtggg
    63301 cgctcacttg tgattgtagt gaacaagtgg gacggcctga gccaggaagt gaaagagcag
    63361 gtgaaagaga cgctggactt ccgtctgggc tttatcgatt ttgctcgtgt gcactttatc
    63421 tctgccttgc acggcagtgg tgttggtaac ttgtttgaat cagtacgtga agcgtatgac
    63481 agctccaccc gtcgtgtggg gacctctatg ctgacgcgca tcatgacgat ggctgttgaa
    63541 gatcaccaac caccgctggt acgcggtcgt cgtgtgaagc tgaaatacgc ccacgccggg
    63601 ggttataacc cgccgattgt ggtgattcac ggcaatcagg tgaaagacct gcctgattcc
    63661 tacaagcgtt acctgatgaa ctacttccgc aaatcgctgg acgtaatggg atcgccgatt
    63721 cgtattcagt tcaaagaagg ggaaaacccg tatgcgaaca agcgtaacac cctgacgcca
    63781 acccagatgc gtaaacgtaa gcgtctgatg aagcatatca agaaaagtaa gtaatcactt
    63841 atctggtttc cctttccaca cggagaggga agccgaataa cacgcgagga gtatttatgg
    63901 cactcaactg tccaacctgc aaaacccctc tcgaacgcaa tggcgagact gctcactgtg
    63961 caacttgcga caaagacttt accgtccagg cactttgccc tgagtgtcat caggcgttac
    64021 aagtgttaaa agcttgtggt gctgtggatt atttctgcca acatgggcac ggtttaatat
    64081 ctaaaaaacg cgttgaattt attattgctt aatatcatgt gaatgtattg
//
LOCUS       DAZGHF010000010        63850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGHF010000010 DAZGHF010000000
VERSION     DAZGHF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.010"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..181
                     /locus_tag="ACHK3A_000710"
     CDS             <1..181
                     /locus_tag="ACHK3A_000710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738843.1"
                     /translation="YDHPYRNDDSRFAKGIIIIVIPFPQRGKSSNCNDVRYGCLDGLS
                     GHYDHSGCTVLCLVA"
     gene            complement(270..662)
                     /locus_tag="ACHK3A_000711"
     CDS             complement(270..662)
                     /locus_tag="ACHK3A_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO8738844.1"
                     /translation="MRDIQQVLERWGAWAANNHENVTWSSVAAGFKGIIPPKITSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            complement(1148..1393)
                     /locus_tag="ACHK3A_000712"
     CDS             complement(1148..1393)
                     /locus_tag="ACHK3A_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738845.1"
                     /translation="MIEEQRERQHKQEATQPDSVTAKNYARVVAEVMRREGTELNGQD
                     CLVIRTRVLGMLAARRRQEQRQNAAPYQWHKPERLRR"
     gene            complement(1962..2393)
                     /locus_tag="ACHK3A_000713"
     CDS             complement(1962..2393)
                     /locus_tag="ACHK3A_000713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738846.1"
                     /translation="MKKIDSLRISKVAIISVSLLFLSACSTNNIENMPRDARVKFALM
                     RAYSDNKFDNSTVIGEVLGLSCARQLGKTLVTPLPGGSAIATSSEGDITSESDAIQDM
                     RYKAAMMGGDAIVNVVCKSGGVDWIHNCWSTVKCVGDVIKK"
     gene            2590..2976
                     /locus_tag="ACHK3A_000714"
     CDS             2590..2976
                     /locus_tag="ACHK3A_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738847.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVRGEIGNPPFDVSGVAYLLYGMD
                     SHNNRYQLESTRRTLEAMVRDGLLERVNVYERREGTGDGVRRKVARYGLPGHSVVVRD
                     GEGRNGAIDGECIRINATDVVLVDGQ"
     gene            complement(3427..3720)
                     /locus_tag="ACHK3A_000715"
     CDS             complement(3427..3720)
                     /locus_tag="ACHK3A_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO8738848.1"
                     /translation="MITDSVAQKLEERGLWRRAATRWAEVLLHAETDSEREEAARRRA
                     ICISKTRRMPEQLVTFGDVKKAADRTLKEMGINPQDEWKNYSFSDAGDDLALS"
     gene            complement(3689..4156)
                     /locus_tag="ACHK3A_000716"
     CDS             complement(3689..4156)
                     /locus_tag="ACHK3A_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO8738849.1"
                     /translation="MNQQTTQQDQTTRRPTERPSWRHMTKRQRKNHRRVNRLCEYWPE
                     LFNLSEVRPLKNGVISDMLQEVRTRNIPVGKGALREAVITYTHMTRYLKALIAGGPRY
                     DLNGQPCGEVTEKEKAAASEQLMWRLEQIKQQNIQWQDTGGGESDNRQRGTET"
     gene            complement(4173..4622)
                     /locus_tag="ACHK3A_000717"
     CDS             complement(4173..4622)
                     /locus_tag="ACHK3A_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8738850.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTAMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            complement(5114..6937)
                     /locus_tag="ACHK3A_000718"
     CDS             complement(5114..6937)
                     /locus_tag="ACHK3A_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO8738851.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWLEQDGKLA
                     GDNIPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLETERKNISDGLVIELPVKKKERGKGDDATSLALNQMGAS
                     QRGEVLLAHYGGELAINADSDTVHYYNSVVWVPVQDKELQRTMAQIFIDEDICYSQNA
                     IKSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFTPPA
                     EGETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGK
                     SVMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAIT
                     GGDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEVVPENER
                     DPMLPEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGY
                     LMSSVMCDGLLVGNAEIIPFSPRRYLYHAYLSYMRAHGFSKPVTLTRFGADMPGAMAE
                     YGREYMKKRTKEGVRSNVILTDESEDWLPSCAPVKNEGDKN"
     gene            complement(6943..7056)
                     /locus_tag="ACHK3A_000719"
                     /pseudo
     CDS             complement(6943..7056)
                     /locus_tag="ACHK3A_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            complement(7123..7317)
                     /locus_tag="ACHK3A_000720"
     CDS             complement(7123..7317)
                     /locus_tag="ACHK3A_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738852.1"
                     /translation="MCNFSDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDMQ"
     gene            complement(7321..7506)
                     /locus_tag="ACHK3A_000721"
     CDS             complement(7321..7506)
                     /locus_tag="ACHK3A_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738853.1"
                     /translation="MLKTFRVFSRAVNKRGHTIGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            complement(7499..7681)
                     /locus_tag="ACHK3A_000722"
                     /pseudo
     CDS             complement(7499..7681)
                     /locus_tag="ACHK3A_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            complement(7724..9316)
                     /locus_tag="ACHK3A_000723"
                     /pseudo
     CDS             complement(7724..9316)
                     /locus_tag="ACHK3A_000723"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9434..9682)
                     /locus_tag="ACHK3A_000724"
                     /pseudo
     CDS             complement(9434..9682)
                     /locus_tag="ACHK3A_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526648.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            complement(9675..10394)
                     /locus_tag="ACHK3A_000725"
     CDS             complement(9675..10394)
                     /locus_tag="ACHK3A_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO8738854.1"
                     /translation="MKRPNMPVQGQGFVQPEISQNDILIRHKAESRIDSRVISERAGI
                     QHESIIATIKSHQSRLRELGSLPRQSVKELTYLKSGKSGRKRGRPEISYLLNELQLDY
                     LLRVIRGRDAERINQFKLDVTKAFAKHRAAEPVRREYLPGYHESRDGLKALGAERHHY
                     INLARTENRFAGLAVGERQSAGEQQLGLLIVMQKIEQAAFNEAIQNGMTPTEAVREVA
                     RRMETFASLMTSAPSLGVNHA"
     gene            complement(10460..10657)
                     /locus_tag="ACHK3A_000726"
     CDS             complement(10460..10657)
                     /locus_tag="ACHK3A_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8738855.1"
                     /translation="MSNNLLRLSDVMRRTGYGRAWIYRLINQGRFPKPVKIGPRSVAF
                     IESEVDEWINQRIEASRNNAA"
     gene            complement(10905..11600)
                     /locus_tag="ACHK3A_000727"
     CDS             complement(10905..11600)
                     /locus_tag="ACHK3A_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738856.1"
                     /translation="MPVVNDFPDLSHWFTVQEFTITQAALLLSGIDPLDYPTLEMVKE
                     EKHERWRMAYGFAYGMVTAIRRGVLTPVLCVAYDWGDMRRLTPAEMMDRSNNISMDGT
                     IITRYSLQEWVKNEKVDYVRPSRSISDKEIKPSNQSVIIDAESSVINSEETVLLTYGH
                     KSEGLEYVEDAIRELWATYDPDNQQTAPTRNDVVEYLKGRGAGQNMAEAVNLILRPTG
                     LQQVGRRKRKTNS"
     gene            complement(11728..12942)
                     /locus_tag="ACHK3A_000728"
     CDS             complement(11728..12942)
                     /locus_tag="ACHK3A_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8738857.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARAARDEAKRLIAGGVNPSDVRKAHKREQASKFGNTFEA
                     IAREWYEKRLDRWSQSYAAEMIDTFEKDVFPYIGSKPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGKCG"
     gene            complement(13107..13180)
                     /locus_tag="ACHK3A_000729"
     tRNA            complement(13107..13180)
                     /locus_tag="ACHK3A_000729"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13146..13148),aa:Gly,seq:ccc)
     gene            complement(13259..14011)
                     /gene="actS"
                     /locus_tag="ACHK3A_000730"
     CDS             complement(13259..14011)
                     /gene="actS"
                     /locus_tag="ACHK3A_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO8738858.1"
                     /translation="MSAGRLNKKSLGIVMLLSIGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKNSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            14427..16724
                     /gene="xdhA"
                     /locus_tag="ACHK3A_000731"
     CDS             14427..16724
                     /gene="xdhA"
                     /locus_tag="ACHK3A_000731"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO8738859.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAISINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAAPIHNGGN
                     LLKQSSMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALNIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            16735..17613
                     /gene="xdhB"
                     /locus_tag="ACHK3A_000732"
     CDS             16735..17613
                     /gene="xdhB"
                     /locus_tag="ACHK3A_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO8738860.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCQLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            17610..18089
                     /gene="xdhC"
                     /locus_tag="ACHK3A_000733"
     CDS             17610..18089
                     /gene="xdhC"
                     /locus_tag="ACHK3A_000733"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO8738861.1"
                     /translation="MNHSETITIECTINGIPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITGIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(18129..19907)
                     /gene="ygeV"
                     /locus_tag="ACHK3A_000734"
     CDS             complement(18129..19907)
                     /gene="ygeV"
                     /locus_tag="ACHK3A_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO8738862.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFISEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            20386..21573
                     /gene="ygeW"
                     /locus_tag="ACHK3A_000735"
     CDS             20386..21573
                     /gene="ygeW"
                     /locus_tag="ACHK3A_000735"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO8738863.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            21631..22827
                     /gene="dpaL"
                     /locus_tag="ACHK3A_000736"
     CDS             21631..22827
                     /gene="dpaL"
                     /locus_tag="ACHK3A_000736"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO8738864.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            22885..24096
                     /gene="ygeY"
                     /locus_tag="ACHK3A_000737"
     CDS             22885..24096
                     /gene="ygeY"
                     /locus_tag="ACHK3A_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO8738865.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            24149..25534
                     /gene="hyuA"
                     /locus_tag="ACHK3A_000738"
     CDS             24149..25534
                     /gene="hyuA"
                     /locus_tag="ACHK3A_000738"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO8738866.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGSNISPQVPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            25582..26514
                     /gene="arcC"
                     /locus_tag="ACHK3A_000739"
     CDS             25582..26514
                     /gene="arcC"
                     /locus_tag="ACHK3A_000739"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO8738867.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(26555..28180)
                     /gene="yqeB"
                     /locus_tag="ACHK3A_000740"
     CDS             complement(26555..28180)
                     /gene="yqeB"
                     /locus_tag="ACHK3A_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO8738868.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTECGFIPVIVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(28228..28998)
                     /gene="yqeC"
                     /locus_tag="ACHK3A_000741"
     CDS             complement(28228..28998)
                     /gene="yqeC"
                     /locus_tag="ACHK3A_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO8738869.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRSECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            29102..29680
                     /gene="mocA"
                     /locus_tag="ACHK3A_000742"
     CDS             29102..29680
                     /gene="mocA"
                     /locus_tag="ACHK3A_000742"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO8738870.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKIMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            30002..33100
                     /gene="ygfK"
                     /locus_tag="ACHK3A_000743"
     CDS             30002..33100
                     /gene="ygfK"
                     /locus_tag="ACHK3A_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO8738871.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSCKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPPELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTELKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVDSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDNEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            33103..34431
                     /gene="ssnA"
                     /locus_tag="ACHK3A_000744"
     CDS             33103..34431
                     /gene="ssnA"
                     /locus_tag="ACHK3A_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO8738872.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKGGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDDAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSHSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLCDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            34482..35261
                     /gene="ygfM"
                     /locus_tag="ACHK3A_000745"
     CDS             34482..35261
                     /gene="ygfM"
                     /locus_tag="ACHK3A_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO8738873.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQTLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEEYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVEKQNLEGDALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            35258..38128
                     /gene="xdhD"
                     /locus_tag="ACHK3A_000746"
     CDS             35258..38128
                     /gene="xdhD"
                     /locus_tag="ACHK3A_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO8738874.1"
                     /translation="MIIHFTLNGTPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEEDNC
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            38293..39693
                     /gene="xanQ"
                     /locus_tag="ACHK3A_000747"
     CDS             38293..39693
                     /gene="xanQ"
                     /locus_tag="ACHK3A_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO8738875.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGATLQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            39711..41027
                     /gene="guaD"
                     /locus_tag="ACHK3A_000748"
     CDS             39711..41027
                     /gene="guaD"
                     /locus_tag="ACHK3A_000748"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO8738876.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            41063..42430
                     /gene="ghxQ"
                     /locus_tag="ACHK3A_000749"
                     /gene_synonym="ygfQ"
     CDS             41063..42430
                     /gene="ghxQ"
                     /locus_tag="ACHK3A_000749"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO8738877.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(42466..42954)
                     /gene="ygfS"
                     /locus_tag="ACHK3A_000750"
     CDS             complement(42466..42954)
                     /gene="ygfS"
                     /locus_tag="ACHK3A_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO8738878.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVSACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(42954..44873)
                     /gene="ygfT"
                     /locus_tag="ACHK3A_000751"
     CDS             complement(42954..44873)
                     /gene="ygfT"
                     /locus_tag="ACHK3A_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO8738879.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQPDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            45308..46756
                     /gene="uacT"
                     /locus_tag="ACHK3A_000752"
     CDS             45308..46756
                     /gene="uacT"
                     /locus_tag="ACHK3A_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO8738880.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            46758..46883
                     /gene="yqfG"
                     /locus_tag="ACHK3A_000753"
     CDS             46758..46883
                     /gene="yqfG"
                     /locus_tag="ACHK3A_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO8738881.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(46880..46951)
                     /locus_tag="ACHK3A_000754"
     CDS             complement(46880..46951)
                     /locus_tag="ACHK3A_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8738882.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            47006..47554
                     /gene="idi"
                     /locus_tag="ACHK3A_000755"
     CDS             47006..47554
                     /gene="idi"
                     /locus_tag="ACHK3A_000755"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO8738883.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     YYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(47597..49114)
                     /gene="lysS"
                     /locus_tag="ACHK3A_000756"
     CDS             complement(47597..49114)
                     /gene="lysS"
                     /locus_tag="ACHK3A_000756"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO8738884.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(49124..50222)
                     /gene="prfB"
                     /locus_tag="ACHK3A_000757"
     CDS             complement(join(49124..50146,50148..50222))
                     /gene="prfB"
                     /locus_tag="ACHK3A_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO8738885.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(50313..52046)
                     /gene="recJ"
                     /locus_tag="ACHK3A_000758"
     CDS             complement(50313..52046)
                     /gene="recJ"
                     /locus_tag="ACHK3A_000758"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO8738886.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHSGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGVA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAL
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLTYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(52052..52762)
                     /gene="dsbC"
                     /locus_tag="ACHK3A_000759"
     CDS             complement(52052..52762)
                     /gene="dsbC"
                     /locus_tag="ACHK3A_000759"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO8738887.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(52787..53683)
                     /gene="xerD"
                     /locus_tag="ACHK3A_000760"
     CDS             complement(52787..53683)
                     /gene="xerD"
                     /locus_tag="ACHK3A_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO8738888.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            53795..54316
                     /gene="fldB"
                     /locus_tag="ACHK3A_000761"
     CDS             53795..54316
                     /gene="fldB"
                     /locus_tag="ACHK3A_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO8738889.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(54356..54763)
                     /gene="ygfX"
                     /locus_tag="ACHK3A_000762"
     CDS             complement(54356..54763)
                     /gene="ygfX"
                     /locus_tag="ACHK3A_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO8738890.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(54744..55010)
                     /gene="sdhE"
                     /locus_tag="ACHK3A_000763"
     CDS             complement(54744..55010)
                     /gene="sdhE"
                     /locus_tag="ACHK3A_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO8738891.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            55263..56243
                     /gene="ygfZ"
                     /locus_tag="ACHK3A_000764"
     CDS             55263..56243
                     /gene="ygfZ"
                     /locus_tag="ACHK3A_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO8738892.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(56320..56979)
                     /gene="yqfA"
                     /locus_tag="ACHK3A_000765"
     CDS             complement(56320..56979)
                     /gene="yqfA"
                     /locus_tag="ACHK3A_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO8738893.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     IKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(57143..57454)
                     /gene="yqfB"
                     /locus_tag="ACHK3A_000766"
     CDS             complement(57143..57454)
                     /gene="yqfB"
                     /locus_tag="ACHK3A_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO8738894.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTDLKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            57499..58932
                     /gene="bglA"
                     /locus_tag="ACHK3A_000767"
     CDS             57499..58932
                     /gene="bglA"
                     /locus_tag="ACHK3A_000767"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO8738895.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLHEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            complement(59096..61969)
                     /gene="gcvP"
                     /locus_tag="ACHK3A_000768"
     CDS             complement(59096..61969)
                     /gene="gcvP"
                     /locus_tag="ACHK3A_000768"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO8738896.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(62088..62477)
                     /gene="gcvH"
                     /locus_tag="ACHK3A_000769"
     CDS             complement(62088..62477)
                     /gene="gcvH"
                     /locus_tag="ACHK3A_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO8738897.1"
                     /translation="MSNVPAELKYSKEHEWLRKEAGGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(62501..63595)
                     /gene="gcvT"
                     /locus_tag="ACHK3A_000770"
     CDS             complement(62501..63595)
                     /gene="gcvT"
                     /locus_tag="ACHK3A_000770"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO8738898.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
BASE COUNT        15543 a        16732 c        15617 g        15958 t
ORIGIN      
        1 ttatgaccat ccctaccgta atgatgattc acgctttgct aaaggtatca tcattatcgt
       61 aatacctttt ccccaacggg gtaaaagctc caactgtaac gatgttcgtt atggttgcct
      121 cgatggtctg agtggtcatt atgaccattc agggtgcacc gtcctttgtc tcgtggcata
      181 attatcaggc gattacgttt ttcatgattg attggcaagc cgtatttctc caccgcagtt
      241 tcctcatgac tgcggttttt ttatgcgcgt tatgccgtca ctgaatcgcg ctcaacatac
      301 cgatccatct ccagcttcac atccagcatc atcaacatgc cttcaacaac cccctccgct
      361 ttgtgaaggc gtttacctat acaggtgtca gagcacccat gtttccgtgc cagcgccatg
      421 aacgtctccc ccaacacgta ataatcaacc agcaagtcat gcagatcgcg attgttccgg
      481 taaaggcggg ctatgcaccc gcatatcacc atcgcatcat cgtcacagca ctgcggacga
      541 gatgttatct ttggcggaat aatcccctta aatccggcag caacggatga ccaggtcaca
      601 ttctcatggt tatttgccgc ccacgctccc catcgctcaa gcacctgttg aatatcgcgc
      661 acgtgttacg ccccctgtga tgtgtttaag gttgtgctat gacttatgcc gattgctgta
      721 aggcccacca gccgcgtaca tcactcgatt gtgagggatg taaaaatcag ggctggctgg
      781 tccggtctgg atggtttaca taccccgata tcggggatgt acaaatcatg gttttacgac
      841 tggtgaattt gaacaactca aataatcgag taaatgagca aaaaacatca atgggttagg
      901 gtaataccac gcagcagtgt ttctcaactc ccaccattga cggaagttga ataaacgggt
      961 aagtccaaag ctcaaaaatg agctttgtaa caatatcagc aagttaccgc gccagcttcc
     1021 accaatggtg gattgcggaa gaatcaatgt attaggccaa agtgcaaatt tgcactctgg
     1081 aacaatatca acaagttaac gcagtcagct acccggctca gaattgagct gcctttaaat
     1141 caacaagtta ccgccgtaac cgctccggct tatgccactg gtacggggcc gcgttctgtc
     1201 gctgctcctg ccgcctcctt gccgccagca taccaagcac cctggtacgg ataaccaagc
     1261 agtcctgccc gttcagttca gtgccttcac ggcgcatcac ttccgcaaca acacgcgcat
     1321 aatttttagc ggtcacgctg tccggctgcg tggcctcctg tttgtgctgg cgttctctct
     1381 gctcttcaat catgaggatt aactcgcgtt cctcctgctc ttcaataatc ccgtgaatta
     1441 tctctgttgg tgaacgttcg gtcatcgggc ctcctgtgtg tcactgtttt ggtgtcaatg
     1501 tcactgttac ggcgacaccg tattttgccc aaatcaggca aaattgctta cccgaatccg
     1561 gtaaatttgc gcgctttttc gatcatcgcg tatcggtttc gttttgcgct tcctgttgcg
     1621 tttcgttttt gagaaaaatg cgcacttttt ccggtggcct ttcccccctg caagccgcgt
     1681 catacctggc ttttctctgc tggtctgtgt gatcggtttt atgatcgatt ttgtgatcgg
     1741 ttttatgatc gatttgcgca gttttttgcg caattttctg cgcatttttt cagggatttt
     1801 tcaccccatc ggaggggctt ccgagggggg gctgtgaagg gggtagcatt ttgctgtgat
     1861 ggaagagggc aattgtaaca ggggtagcta acggtaagct gcaatcacac gtcgtaattg
     1921 cagtttccat gtattaatca ataccttcag tatggcaact gttatttttt tattacatca
     1981 ccaacgcact taacagttga ccaacagtta tgaatccagt caacaccacc tgatttacat
     2041 acaacgttca ctatggcatc acctcccatc attgcggctt tatatcgcat gtcctgtatt
     2101 gcatccgact cagaagtaat gtcgccctca gaggatgtag caatagcgct tccccctggc
     2161 aatggtgtta ctaatgtttt tccaagttgt ctggcgcaac tcaatcctaa cacttcgccg
     2221 ataactgttg aattatcaaa cttgttatct gaataagccc tcattagagc aaattttaca
     2281 cgcgcatctc tcggcatatt ctcaatatta tttgtagaac acgcgctaag aaataataaa
     2341 gatactgata taatagctac tttacttatt cgtaaagaat caattttttt catcttgcgt
     2401 acctcacaac acaacaattt gaatattcat atttgcctca tattgccgca acccatcgca
     2461 taacccttgt gcttccagcg cttcagcctt gtcatcacgt atcatctgta ccccgccgat
     2521 tctgtccttt caaaatttgc cttatggctt attgtatgag ggattcatcc tacaacaaga
     2581 aaggtcacta tgagactgac gcaatacaaa aaagagatcc tcagttactt cactcccgac
     2641 aatctggcgt gggtcagagg tgaaattggt aacccgccgt ttgatgtctc cggtgtggct
     2701 tacctgctgt atggcatgga ttcacacaac aaccgatatc agctcgaatc aacaaggcgc
     2761 acacttgaag cgatggtccg tgacggtctt ctggagcggg ttaacgtgta tgagcggcgt
     2821 gagggaacag gtgatggtgt tcgtcgaaag gtggcgcgtt atggcctgcc tggtcattct
     2881 gttgtggtgc gtgacggtga gggccggaac ggtgcaattg acggcgaatg tatacgcatc
     2941 aatgctacag atgtggtgct ggtggacggg caataaaaaa acctgaataa ctccgctggg
     3001 tgcttcagtt gcacggagcg aaatcattca gggtcagccg taacgggtct ctgtacaggt
     3061 ggcttcagtt gccagaagat gagcgcccgt tacagcctgc tgtgataagc cgctttacag
     3121 tgagcttcaa ttgctcaaag aggcgcgttt ttatcacaac tttgccgcaa taacccgctg
     3181 tacagagggc tccagttgcc caaagaggcg cattcttatc gcggcatgac gaaatcaccg
     3241 tgatgttttc gtcgttcact catcccgatg tgtgtgacaa gtacacatca aaatgagtgc
     3301 cgaaaagccg ctggctggct tcattcggcg aggctcccgt tgcctgcgga gaacaccatc
     3361 aacggccgtt cggtggtgat tatagcgaat cgcgataatt gagcaaatac ggcgatgata
     3421 tgcgtatcac gatagcgcta aatcgtcgcc tgcatcactg aagctgtaat ttttccattc
     3481 atcctgcgga ttaatcccca tctctttcag cgttctgtct gcggcctttt tcacatcgcc
     3541 aaaggtgacc agttgttcag gcatacgacg ggttttactg atgcatatcg cccgacgtct
     3601 ggcagcttcc tcgcgttcac tgtccgtttc tgcgtgaaga agaacctcag cccatcgggt
     3661 cgccgcacgt cgccataaac cacgctcctc aagtttctgt gccacgctgt ctgttatcac
     3721 tctcaccacc tcccgtatcc tgccactgga tgttttgctg ttttatctgc tccagcctcc
     3781 acatcagttg ttcgctggct gctgctttct ctttctccgt gacttcgccg caaggttgcc
     3841 cgttcaggtc gtaccgtggt ccaccagcaa tcagcgcctt aaggtatcgc gtcatgtggg
     3901 tgtaagtgat taccgcctca cgtaatgcac ctttgcctac aggtatgttt cttgtcctga
     3961 cctcctgcaa catatcgctg attacgccgt tcttgagggg cctgacttcc gagaggttaa
     4021 acagttcagg ccagtactcg cacagacggt ttacgcgtct gtggtttttt cgctgccgct
     4081 tcgtcatgtg tcgccatgat gggcgctccg tgggtctgcg agtggtctgg tcctgttgtg
     4141 tggtctgctg gttcatgttg tgctcctgtc attcagaaag gaatatcgtc agagaacggg
     4201 tcatcacctc ccgctggtgg ctgatttccc tgctgtccgg tctgtcgttt tgctctttcc
     4261 agtgcttcgg tcgccttccc ctgttgcccc cgcttcccgc caggacgcgc cgaacgggca
     4321 ctgattacgc tgtcagcgat aacctgccag ccctgccgta tttctccgtt ctgtccggtc
     4381 cactggctga tctgcatgtt acccgccacg ctgataaggt cacctttttc atgtctcgcc
     4441 agcgcgtcgg cctgcttacc aaatgccagg actgatagcc ataacgtcgc ctgtccgtcc
     4501 tgtgcctgac tgcatggcag tgataccgcc atacgcgcca gcgtcatcgc tgttcccttg
     4561 ctggtctgtt ttacctgcgg gtcgtccacc agccgcccgt aagctgcaat ttgtgctgtc
     4621 atggtctgcg ctccttgttc cggtactcct ggataatccc cattgtcata tcgaataaat
     4681 cacatgccgc tttttctgca tcttccgctt tcaggtcata cagcgtcatg aaatggtgag
     4741 cgattgcccg tgcggatctt ttgctgatcc ctgttcggtt aaatcgatgg cgtgtgagtg
     4801 tgatttttta gcgtgtttct ctgtcataaa atttttctca gaggaataaa taaaaattta
     4861 tacccaaaag gtgttcaact gttcaccttt tgctatttca cctttagatt cataaagtta
     4921 aggggtgaac actttttgta aaggtgttca ccaactgttc accctgttca cccgcgactg
     4981 ttcaaaaatc atacaaaaag gtgaacaggt gaatactttg gtgaatagtt ttacaaaagt
     5041 gttcacccat taacatattg atactaaata catttattgc agggtgaaca gtggtgaaca
     5101 gtttttctat aacttaattt ttatcaccct catttttcac tggtgcacat gacggcagcc
     5161 agtcctcaga ctcatccgtc agtatcacgt ttgatcgcac accttcttta gttctctttt
     5221 tcatatactc cctgccatat tccgccattg cgcctggcat atctgcaccg aagcgcgtca
     5281 gtgttacggg cttactgaaa ccgtgcgccc tcatatacga gagataggcg tgataaagat
     5341 aacggcgtgg actgaaagga ataatctcag cattaccgac taacaggccg tcacacatta
     5401 ccgacgacat gagatagccg cagaagtcca ccagcgaatc cccctcacgc tttatcgcca
     5461 gtgcttcttc tgatttctgc tgctcatata aaaggcgtct ggcttcgtcc tgatcagcaa
     5521 accgtgtaag cagatgacga atcactacag caagctcacc ttctattttt tccggcagca
     5581 tcggatcgcg ttcgttttcc ggtacaacct ccgaaaaatt gaatatcaca cgacgacgtg
     5641 agatcccccc gctgcggtca ctgaatgaca tggcgctgtt gttaaccgcc agcacaaccg
     5701 ccggaatacg cgttgagtat ggcgctttgt gtttcgggtc aattgccacc ttgtcaccgc
     5761 ccgtaatggc ctttatccct gcaccatcac ccgcgtagcg ggtcatatcc ggcagaataa
     5821 tcagtgaaaa gccaaccact aatgcacgtt cccttgcatc ctccagtgcc ttcatgcttg
     5881 ccgataccgt gttggcctta cctgccagca tggtacaaat ctctgccata acacttttac
     5941 cgcttccacc aggacctgtt acctcaatga ataactgcca gtcgtaccgg ttcgccagca
     6001 ccataaataa cgctgccaga acgcgatctg ccttgcggtc attctcagcc accgaacggc
     6061 gcaaccattt ccagaaattc ggcgcatgtg ttgccagcgt ttcaccctct gctggtgggg
     6121 tgaacggtaa ttcactggca attaacagcc agtcattttt atcgtgttca cggaaatcac
     6181 ctgtacgggt atcaaatacc ccgttactga atccaatcag gttgcgggcg gtattcccca
     6241 ttacgggtaa acttaacttc atggtatcaa ccgccgattt tatggcgttt tgtgaatagc
     6301 agatatcttc gtcaatgaag atttgcgcca ttgtgcgctg tagctcttta tcctgtacgg
     6361 gaacccatac aacactgttg taataatgca ctgtgtcaga gtcggcgttt attgccagtt
     6421 cgccgccata atgtgccaga agaacttcgc cgcgctggct tgcccccatc tggtttagcg
     6481 ccagtgatgt ggcatcatca ccttttcccc gctctttttt ctttacaggc agctcaataa
     6541 ccagcccgtc agaaatgttt ttgcgctcag tctccagata gttacgccag ttctgccgct
     6601 cgttatggtg cataccttcg gggtaatagt ctgcgtcctg tacgcctgcc gccgccagct
     6661 tctgacctat gcctttgacc atcacctgat cgaggtgtcc ggccttgtat atacgcgctg
     6721 atttgcgccc gtcgggtaca atttgcagat tgccaatatc tttaagttgc tcatcagcca
     6781 gcacgacggg cggtatatta tctccggcaa gttttccgtc ctgctccagc cactgtttcg
     6841 cgtgcgccca cgcatcactg ccagcgaaaa taatcacttc cgtatcggcg tgttttttcc
     6901 ctcgaggctg tttttttaag ttcggtgcgc ttttcatttc ttacctctgc tgatcatcgt
     6961 catgtggcta ccgtattcat tgcggtaaat atccccgcac ataaattcgg ggtgagtttt
     7021 gctactggca gttaagccag aatatttacg tttcatcgtt ttatttcctg atatgtaacg
     7081 ctttaattcg ctggtattta tttcatcctg ttatttattc ccttattgca tatcacccga
     7141 tgccgcaatc agccagctac cagccttacc tgtaagctgc ttaatcaggg cgagcaatga
     7201 ctcaatctca ccctcagtca tttcacctgt gttacccatc atcagagaac aaataagttc
     7261 tgcctgcatt gcgctttttg ctgcttcatc aacagtcata tcggaaaaat tacacatgaa
     7321 ttactcctca tcctgaatac atgtcactgc gtggataatt acgccggata aaccggcttt
     7381 agctgatttg ttaattactg tgctgattgc tgcctggacg ttttcggcct tcacgttctg
     7441 tgtgatgccg atggtatggc cgcgcttgtt aacagcacgg gaaaagacgc ggaaggtttt
     7501 aagcatgact caccccctga cgaatacggg cgacaaaaat cagatgtgcg tgcggcagcc
     7561 gggcgcgtgc ttcgcgctca gtggcagcgg taacggtgaa tattgcaatg ctgcctgtca
     7621 ggcactgcat aaagcgccag acaaagtaag ggcgtgcgga tacagccatg tgaaaggctc
     7681 ctctgacttt gggattatac ccaccgccgg aggtggtaag ctcgctggcg gtggactgta
     7741 cagggttacc acaactggcg ttagaggaaa ccagcccgac cgaagtcggc cccatacagc
     7801 ccaccattgt tgaggtgtgc gtgtgcgccg atacaaaaaa agacgctggc gcgtcgtgta
     7861 tcgcctctaa ctcctgcggg gtggtaatcc cgatccccgt tttataaggg atagcctcag
     7921 tatatgccgc ttttttgctt aagtggtcgg atgtgttgcg agaaaagttt gtgcgaaaaa
     7981 tattcacata tccttccaga aaggatggat accagaaaga aagacaacgt accagacaat
     8041 aaccagcgcc gtgatatgag gcatcacgac ataatgcatt accggaatcc agtatgagta
     8101 agcacagatt ttcgcccgtt accccgaata tcagcgcact acaacgccag cgactgatac
     8161 gtgacatcat tgaaaccagg aaagaaaaat taaccccgcc agaaaaaacc agcgaaccgc
     8221 caccgcttga ggtgtttctc agtggcagaa aaaaccaacg caataaatta caacaacaga
     8281 aaccatcatc gctgtagcgt ggtgtgttac ggcgtattgc tttacaggta cagacaatga
     8341 caaatgaaga actacaggaa atcgcattat ccatacttga gcgacagcgc gaaaaagcac
     8401 aacggcaaag aaggttgtta cttaatccac agccactcac caggccaaca cgatttgccc
     8461 ttgaggcttt tgtcgagaaa tacccacggc ggttaaaagc cactaaaaac cgaccgccag
     8521 gctgatagct cgctgtttct tctgcggctc tatgaaacag acagaaacca tcagcgccgt
     8581 gatgtgagga agtacggcgc attgttttac aggtgcacaa tatggataac acacaacaca
     8641 cttcacaatc agagaataaa aaagatgatc tcacagcata tgagcaacat ttcgttgaaa
     8701 aaaaacagtc aataacgaga cacatcgcag atatggatat gcgcaagcga tttgaaaaaa
     8761 ctttcaatac caattttgcg cttgaggatt ttttaaagca gcattccagc cgatcaagca
     8821 ataagggata gcttatggat gaccagaaaa aaaactcctt tgccatgatg gttacactga
     8881 ccagcgcccg tataactgaa catgaacgaa agcggaaagc tgcacgcgaa caggcaagag
     8941 caacagaatt aacacgtaaa gaacttaacg ctctggcacg ggaacacaga gaagcacctg
     9001 aagcttttgc acttgataat tttctgaaaa gtacatgccg taagaaaata cgaacatcgg
     9061 gaccaagggg ccattcgtcg taaactgcat attgtccgcc agcctcagat atggcattgt
     9121 ggataatgcg cttgatttcc tttatctcca ctggcgaccc tttcgtggtc gcctttgttt
     9181 tgcctgccat tcctgcatca tgcggatatt cgcgccgtac agggccacca gcgcgataat
     9241 ttgataaacc ctcccccaag tattgcttaa cgtgtttcat gcgctcagcg gtgaaatggc
     9301 tcacgatatg gcgcatgtaa tcagcatggt tgatgctcac ctgtgcgaac ttatccgcag
     9361 tatcgatgta cagggcggca gcgttgcgga tgtcaggtat gttcacgttc tcccaggcga
     9421 cgtgccagcc agaatcaaac tgttcaatat ttacactttg gctcccccgt ttcggggaat
     9481 caaagtacaa attttctaca acctcaaaat ccacaccttt tttaaatccg tactcctcaa
     9541 tacgcccttt gatccaggtg gtgaaatcac ggcctacacc gagcgcttta tgcagtgcgc
     9601 gtgcgctgac aacgcccgtt tcatatctgc caataatgcc aggtgatacc ggaataattg
     9661 cggcgaaatc ataattacgc atgattcacc cccagagacg gcgctgacgt catcaggctg
     9721 gcgaacgtct ccatgcggcg agcaacttcc cgcaccgctt cggtcggtgt catgccgttc
     9781 tgtatcgcct cgttaaatgc tgcctgctcg attttctgca tgactatcag taagcctaac
     9841 tgttgctctc ccgcgctctg acgctcgcct acagccagcc cagcgaagcg attttcagtg
     9901 cgggcaaggt tgatgtaatg gtgacgttct gcgcccagtg ctttaagccc gtcacggctt
     9961 tcgtgatagc ctggcagata ttcgcgacgg actggttcag cggcgcgatg cttcgcgaat
    10021 gccttcgtaa catccagctt gaactggttg atacgttctg cgtcacgccc acggataacg
    10081 cgtaacagat agtcaagctg tagctcattc agcaaataac taatttcagg acgaccgcgc
    10141 ttgcggccag attttcccga tttcaaatag gttaattcct tgactgactg gcgaggcagt
    10201 gagccaagct cgcgcaatct ggattgatga gatttaatcg tcgcaatgat actttcgtgt
    10261 tgtattcctg ctcgctcaga aataacgcgg gaatcaatac gcgactccgc tttgtggcga
    10321 atcaatatat cgttctggct gatttcaggc tgaacgaagc cctgcccctg tacgggcata
    10381 ttcggacgtt tcattgaaaa actcctgcta tcggatcagg tttagtgctg ggggagaata
    10441 tccccctccc aaaagattat tacgcggcgt tgttgcgaga agcctcgata cgctggttaa
    10501 tccattcatc gacttcgctt tcaataaaag ccacggagcg gggaccaatt tttacaggtt
    10561 taggaaagcg cccctggttg atcaggcgat aaatccaagc gcgaccgtaa ccagtacggc
    10621 gcataacatc agaaagacga agtaagttat ttgacatatt caatcctcgt aacgtctata
    10681 accgtctaca atgatgcata aaaggaaaac atatttatag gtggatacta tttttaatgg
    10741 tgggtactat ttcaatggaa tagtgcccac tattatttta ataatgctgc caccattcca
    10801 gtatcaataa gcaaagaaaa cgcacgctta actatgattt ctcgccataa acaaaagagt
    10861 tttgagcaaa tagtgccacc ttttttacga agggtgggca ctatttaact atttgttttt
    10921 cttttccttc ttccaacttg ctgcaagcca gtggggcgta gtatcaggtt tacagcctcg
    10981 gccatgttct gccccgctcc cctgcctttc aggtattcaa caacatcatt tcttgtaggg
    11041 gctgtttgtt gattatctgg atcatatgtt gcccaaagtt ctcttattgc gtcttctaca
    11101 tattcaagac cttcgctctt atgaccataa gtcagcaata ctgtttcttc agaattaatc
    11161 acgctagatt cggcatcaat tattactgat tggttagatg gcttaatctc cttatccgat
    11221 atagagcgag agggtcttac ataatcaact ttctcatttt ttacccattc ctgtaatgaa
    11281 taacgggtaa taattgttcc gtccatggat atattatttg aacgatccat catctctgct
    11341 ggggttaaac gacgcatatc cccccaatca tacgcaacgc acagaacggg agttaagaca
    11401 ccgcgtcgaa ttgcagtaac cattccataa gcaaacccat atgccattct ccatcgttcg
    11461 tgtttttcct ctttgaccat ctccagagtt gggtagtcga gaggatcaat acctgacagc
    11521 aataacgcag cttgagtaat agtaaactcc tgcactgtaa accaatgact taagtcaggg
    11581 aaatcattca caaccggcat gtgcacacct cgcactctct gtagtggtgg ctgtgccagt
    11641 ccgcagaggt gtacggggtt tcgggagcga ccctagacac agcctgttat ttagtcgctc
    11701 aaagtctact actgtatact caaccagtca tccacatttt ccaaacttgc catgtaccac
    11761 gtttccgccc tgttccagtg agtccatata gtcggcgtac cactggagca tctcccgccg
    11821 tccgtccaga tattgcgcgt gattgtatgt gccacggatc gagtttttat cgacgtgcgc
    11881 cagctgcgtt tctatccatg cggagttata cccctgctcg tgcaaaatcg tactcatggt
    11941 gtgccggaac ccatgaccag taacacgccc tgcataacca atacgacgaa taagcacgtt
    12001 catcgccatt tcactcatcg gtttactgtg ctgtattcga ccagggaaaa taaacggata
    12061 ctgccccgtc atgtgtttca gttgttccag aatagctatc gcctgttctg aaagcggcac
    12121 gcaatgagga cggcgcattt tcattcgttc tttcggcact tcccacagac gattatcaaa
    12181 atcaatctcc tgccactctg cctgccgcaa ttcacctggg cggagtcctg taagaatcaa
    12241 taatcgcata cccagcttga cgatctggct tccggtgtac gtattaaaaa cgcggaaaaa
    12301 ttccggtagc tcatcaactt tcagatacgc gtaatgctct tttttgtgtg gtgcaaatgc
    12361 gcttgccaga tcaggagcgg ggttatattc agcgcgtccg gtaattattg cgtaccggaa
    12421 cacttcgcca catcgctggc gaaccttacg caatttttca gttgcgccac ggtcattaag
    12481 acgggagaga accgcgagca attccatcgg ttttatttct gctatcggtt tgctgccgat
    12541 atacgggaat acgtccttct caaacgtatc aatcatctct gcggcatatg attgtgacca
    12601 tctgtccagg cgtttttcat accactcacg ggcgatcgcc tcaaaggtgt taccgaattt
    12661 gctcgcctgt tctctcttat gggcttttct cacatcactt ggattaactc caccagcgat
    12721 aagccttttg gcttcatccc ttgccgccct ggcatcagaa agggacacct caggataaac
    12781 acccagcgac aacattttgg gcttacctgc aaacctgtat cgcaatcgcc agcccttcga
    12841 accgttaggc tcaatgagta acgaaagccc gttgccgtca ttgagtgtat aggctttctc
    12901 ttttggcttc gcgcggcgga tagcaaggtc tgaaagcaac atgtgtatag cccccaaaaa
    12961 acgattttgt cctatacgca ttactataca cttctgtgtg tagatttagg tagatgactg
    13021 tagacgcttg tagacgagga aaatcgctgg aggccttgat attcggggga ttggcagact
    13081 ttagtagacg ttggtagatg tttgtttgga gcgggcgaag ggaatcgaac cctcgtatag
    13141 agcttgggaa gctctcgttc taccattgaa ctacgcccgc ttcgagatgc gtaaggcatt
    13201 ataaacctta cgctctcctt agcaagtgcc gcgctgctga ctgctgatta attcgccatc
    13261 agcattttgg cttgctgccc tgaggcggca agtaacgtag cggatcaatt gccgttgcac
    13321 ggtaacgaat ctggaaatgc aggcgaaccg atgccgcatc agtgctcccc atagtggcga
    13381 ttttttgccc agccttcacg ctttgcccat tatttaccag catcgtgtca ttatgggcgt
    13441 aagccgtaat gtaatcttca ctgtgtttaa tcatgatgag attaccgtag ccacgcagct
    13501 ggttgcccac atacaccacc tttcctgcac ccgcggcgta aataggtgta ccccgtggag
    13561 ctgagatatc aatcccttta ttgccgccat cagctgtcga atacggcatg ataactttcc
    13621 ctgtcgttgg ccataaccaa caacgttgcc ctactggcgg ccaggaagat ttcggtactg
    13681 ctgatgacgg tgtaaccgat gcggttttgg tcgttgattt ggcggttgat ttacgtgtac
    13741 tactattttt cgccccaccc agttttagtt tctgaccaac ttcaatggtg taaggggggg
    13801 aaatgccgtt cagtcgcgcc agctctttta cgctagttcc cgtggtgcgc gaaatacgat
    13861 atagcgtatc cccccgtttc acggtgtaaa cggagccgga atacgttcct gtatcggatg
    13921 atttgctacc cgagcagccc gccaaaagca gtccaatcga taacaacatc acgataccca
    13981 gagatttttt attcaggcgt cccgcactca aaatctttcc tcgcttaaaa aacaagacgc
    14041 catatcatag cagccagcct cttgacaccc aatctcttgc ttctggaaaa acgcggtaat
    14101 agagaaagag gttaatttca tttggcaaag ggaagtatca tcagcaaaat attcactgta
    14161 gatgtatcga attttctgac tcatttaccc tatttcccat cctgatgcgt aataatccca
    14221 atttatcaca ttgattttat cgccactcat attgatacgt atcactgccc ctattcaggg
    14281 tcctgtttcg tatcatatct gtgttttttt aacatcatca cacttccttc ctcccttaac
    14341 gcttatcgtc gtttctggcg taaatcttgc ctgcttagac taaatctttg ccataagaac
    14401 caaatgttca aatcgagggg atttctatgg aagcgcggga agcaaccgct acaggtgaat
    14461 catgcatgcg cgtagatgcc attgctaagg tcaccgggcg ggcacgatat actgacgatt
    14521 atgttatggc gggcatgtgt tacgcgaaat atgtacgtag ccctatcgca catggttatg
    14581 ccataagtat taatgatgaa caagccagaa gtttaccggg cgtactggcg atttttacct
    14641 gggaagatgt gcctgatatc ccattcgcta cagctgggca tgcctggaca cttgacgaaa
    14701 acaagcgcga taccgccgat cgcgcactgc taactcgcca tgttcgtcat catggtgacg
    14761 ccgttgccat cgtcgtggcc cgtgatgaac tcacggcaga aaaagcggcg caattggtca
    14821 gcattgagtg gcaagaatta cccgttatca ccacgccaga agcggcgctg gcagatgacg
    14881 ctgcaccaat ccataacggc ggcaatttac taaaacaaag ctcgatgtcg acgggtaatg
    14941 tccaacaaac aatcgatgcc gccgattacc aggtgcaggg gcactatcag actcccgtta
    15001 ttcaacattg tcatatggaa agcgtgacat cgctggcatg gatggaggat gactcgcgaa
    15061 ttaccatcgt ttccagcacc cagatcccgc acattgttcg ccgtgtggtt ggtcaggcgc
    15121 tgaatattcc ctggtcatgc gtacgagtca tcaaaccgtt tgtcggtggc ggttttggta
    15181 ataaacagga tgtactggaa gagccaatgg cggcattcct gaccagcaag cttggcggca
    15241 ttccggtgaa agtttccctt agccgtgaag agtgtttcct cgcaacccgt acccgccacg
    15301 cttttactat tgacgggcaa atgggcgtga accgcgacgg aacattgaaa ggttatagtc
    15361 tggatgttct gtctaacacc ggcgcttatg catctcacgg gcactccatc gcttctgcgg
    15421 gcggaaataa agtcgcttac ctttatcctc gttgtgccta cgcttacagt tcaaagacct
    15481 gctataccaa cctcccctcg gctggtgcga tgcgtggtta tggcgcgcca caagtcgtat
    15541 ttgccgttga gtctatgctt gatgacgccg cgacagcgtt aggtattgat cctgttgaaa
    15601 ttcgtttacg caacgccgcc cgcgaaggag atgctaatcc gctcacgggc aaacgtattt
    15661 acagcgcagg gttgccggag tgtcttgaaa aaggccggaa aatctttgaa tgggaaaaac
    15721 gccgtgcaga gtgccagaac cagcaaggca atttacgccg cggcgttggc gtcgcctgtt
    15781 ttagctacac ctctaacacc tggcctgtcg gcgtagaaat agcaggcgcg cgcctgttga
    15841 tgaatcagga tggaaccatc aacgtgcaaa gcggcgcgac agaaatcggt cagggggccg
    15901 acaccgtctt ctcgcaaatg gtagcagaaa ccgtgggggt tccggtcagc gacgttcgcg
    15961 ttatttcaac ccaagatacc gacgttacgc cgttcgatcc cggtgcattt gcctcacgcc
    16021 agagctatgt tgccgcgcct gcgctgcgca gtgcggcact gttattaaag gagaaaatca
    16081 tcgctcatgc cgctgtcatg ctacatcagt cagcgatgaa tcttaccctg ataaaaggcc
    16141 atatcgtgct ggttgaacga ccggaagagc cattaatgtc gttaaaagat ttggcgatgg
    16201 acgccttcta ccaccctgaa cgcggcgggc agctctctgc cgaaagctcc atcaaaacca
    16261 ccactaaccc accagcgttt ggctgtacct ttgttgatct gacggtcgat attgcgctgt
    16321 gtaaagtcac catcaaccgc atcctcaacg ttcatgattc gggccatatt cttaatccgc
    16381 tactggcaga aggtcaggta cacggcggaa tgggaatggg cattggctgg gcgctatttg
    16441 aagagatgat cattgatgcg aaaagcggcg tggtccgtaa ccccaatctg ctggattaca
    16501 aaatgccgac catgccggat ctgccacaac tggaaagcgc gttcgtcgaa atcaatgagc
    16561 cgcaatccgc atacggacat aagtcactgg gtgagccacc aataattcct gttgccgctg
    16621 ctattcgtaa cgcggtgaag atggctaccg gtgttgcaat caatacactg ccgctgacgc
    16681 caaaacggtt atatgaagag ttccatctgg caggattgat ttgaggataa catcatgttt
    16741 gattttgctt cttaccatcg cgcagcaacc cttgccgatg ccatcaacct gctggctgac
    16801 aacccgcagg ccaaactgct cgccggtggc actgacgtac tgattcagct ccaccatcac
    16861 aatgaccgtt atcgccatat tgttgatatt cataatctgg cggagctgcg gggaattacg
    16921 ctggcggaag atggctcact acgtatcggc tctgcaacga catttaccca gctcattgaa
    16981 gatcctgtaa ctcaacgtca tctcccggcg ttatgtgctg cggcctcatc cattgccgga
    17041 ccgcagatcc gtaacgtcgc tacctacggt ggaaatattt gcaacggtgc caccagcgca
    17101 gattctgcca cgccaacgct aatttatgac gcgaaactgg agatccactc cccgcgcggt
    17161 gttcgtttcg tcccgattaa tggctttcac accgggccgg gcaaagtgtc tcttgagcat
    17221 gacgaaatcc ttgtcgcctt tcattttccg ccccagccga aagaacacgc gggcagcgcg
    17281 cattttaaat atgccatgcg cgacgcaatg gatatttcaa cgattggctg cgccgcacat
    17341 tgccaactgg ataacggcaa tttcagcgaa ttacgcctgg catttggtgt tgccgcgcca
    17401 acgccaattc gctgccaaca tgccgaacag actgcacaaa atgcgccatt aaacctgcaa
    17461 acgctggaag ccatcagcga atcagtcctg caagatgtcg ccccgcgttc gtcatggcgg
    17521 gccagtaaag agtttcgtct gcatctcatc cagacgatga ccaaaaaagt gattagcgaa
    17581 gccgtcgccg cggcgggggg aaaattgcaa tgaatcacag cgaaacaatt accatcgaat
    17641 gcaccattaa cgggatacct tttcagcttc acgccgcacc aggcacgccg ctctcggaat
    17701 tactccgcga acaaggactg ctaagtgtca aacaagggtg ctgcgtgggt gaatgtggtg
    17761 cctgtacggt gctggtcgac ggcacagcaa tagacagttg cttatacctt gccgcctggg
    17821 ctgaaggaaa agagatccgc acgctggaag gtgaagcgaa aggcggaaaa ctttctcatg
    17881 ttcagcaggc ttatgcgaaa tccggcgcag tgcagtgcgg gttttgtacg ccgggcctga
    17941 ttatggctac cacggcaatg ctggcgaaac cacgcgagaa gccattaacc attacgggaa
    18001 ttcgtcgtgg actggcggga aatctttgtc gctgcacggg gtatcagatg attgtaaata
    18061 cagttctgga ttgcgagaaa acgaagtaaa aggatatccg gcctgaattc aggccggatt
    18121 cactgaggtt atgtgtttaa caactcatat ttcttaatct tgcgatagag cgtagcaatg
    18181 ccgatgccca gttcatcagc aacttccttc ttgctgttat gacgtgaaag cgcctcgcgg
    18241 atcatttgct tttccatctc ctccagcgcc gtgccgcccg catcatcgag tgccaggtgc
    18301 gcctcactga cctctgttac atcactttgc tccgttgtgc cattattcag cagatttggc
    18361 ggcaatagcg tgctgtcgat aacttcacct gaaggaacca cgttaaccag atattccatc
    18421 aaattgctta actcgcgcag gtttccgggc caacgatgct tacgcaatat ttcgacgaca
    18481 tcgggagcaa tgcccggata aaccgatccc agacgacggg tatgcagatg taaaaagtaa
    18541 tgcaccagta gttcaatatc ttcctgacgt tcacgcagcg gtggcagagt tatcgggata
    18601 acattaagtc ggtagaagag atcttcgcgg aatttacctt cggaaatgaa ctgggccaaa
    18661 ttctggttag ttgcagaaat gatgcgaatg tcgacttgta ttgggctact ggcaccaatc
    18721 ggcagaattt cacgtgcctc aatagcgcgc agtaatttag cctgcaacat taatggcata
    18781 tcacctattt catcgagaaa cagcgtgccc gtattcgccg cctgaatcaa ccctgtttta
    18841 ccgttggcag aagcgccagt aaatgcgcct ttaacataac cgaacagttc gctctccaga
    18901 agctgctccg gaatcgcggc acagttgata gcaataaaag gtttattccg tcttccgctc
    18961 aacttatgga ttgcacgggc gacgacttct ttacccgtgc cgctttcgcc aaccaccata
    19021 acgctggatg ggctgggtgc aatacggcta atgagtcgtt ttaattgccg cataacacgg
    19081 cactcgccaa ccaattgttc aatatgcggt tcatcaggtg catttgctac agaaaaactg
    19141 gtatgcgatt ggtgaaacgc cattaaaaat aattgtcggc cctgaatgtt atgcaattga
    19201 ccaatgatta attcactttt atcgtcccac gaaacaatat gctgcatatg tccatgggta
    19261 aaattactct caaatgttaa tggtctgaaa cgaacaggtt tcccaataat attattttgc
    19321 acaacaccga gtgtttttaa ggcagtctga ttaacaaact gaacccgatt ttcgtcatca
    19381 acaactaaaa cgccctgatc catattatcg atcatggtcg cgaatatttt actgatgtta
    19441 tctcctggcc cctgatcctc cagaagtttc gaaacaaaaa tggtggatat atggcgaacg
    19501 taatcagaaa attcgcgtaa attatcactg atatgttctt gttgctcgtg ggtaacggca
    19561 atcaaactta tcaccccaac acaacgatcc tgtaaaatga caggcgtacc cagaaatgct
    19621 ttttcgcggc aattttcttt actatcgcaa ccttcacaaa ggggatcgaa gcgagactgt
    19681 gtcacaactt tttcagtttt cgtttccagg acgtggcgga gcaggcgtga gttgccgctc
    19741 aactggcgac caagaaactt cccatacgcg cccgttccgg cgacgcgaca caagttttca
    19801 tcaacgatct caacctcaag ctgcaaaacg ctggcaagca ttctggcaaa acgctgaatt
    19861 gtcggttgaa tttgcatcaa tactgactgc gtagtagcaa gctccatagc tttaccttcc
    19921 agacttactt aaaagtcgat cattgaagac gttgatggtt catagatcat gatgatatta
    19981 actcaggcga aattggcttt gataaaaaca taagattttt atcactttct aatgaaatta
    20041 tggaagagat atcacatttc tatatcaata tgagaattac ggcggtgagt ttatcaaact
    20101 gaagagagat cgcctgtccc tttatcttat ttctgatact tagcagcaaa taaataacgc
    20161 gataaaaaaa gccaaacgtt ttcgtatttt acaaacaacc agaagctggc atcaatttgt
    20221 gatcaacccc acacattatc cgtcaaatta gtcttttgca gccccgtgga taattctggc
    20281 acacttattg ttagtcccag gtatagctgt gaaaacacca atcacgttgg caagtcacag
    20341 tgaaataaac cactttgcct gtcattccac taccgggact ttatgatgaa aactgttaat
    20401 gagctgatta aggatatcaa ttcgctgacc tctcaccttc acgagaaaga ttttttgtta
    20461 acgtgggaac agacgccaga tgaactgaaa caagtactgg acgttgccgc agcattaaaa
    20521 gcactgcgtg ctgaaaacat ctcaaccaaa gtctttaata gtggattagg tatttccgta
    20581 ttccgcgaca actccacccg tacccgcttc tcttatgctt ccgcgcttaa cctgctcggc
    20641 cttgcacagc aagatctcga cgaaggcaaa tcacaaatcg ctcacggcga aaccgtgcgt
    20701 gaaaccgcca atatgatctc cttctgcgcc gatgccatcg gtattcgcga cgatatgtat
    20761 ctgggcgcag gcaacgccta tatgcgtgaa gttggcgcgg cacttgatga cggctacaaa
    20821 caaggggttc tgccacagcg tccggcttta gtgaacctgc aatgcgatat tgaccacccg
    20881 actcagtcta tggctgacct ggcatggttg cgtgaacact ttggttcgct ggaaaacctg
    20941 aaaggcaaaa aaatcgccat gacctgggcc tactccccaa gctatggcaa accgctctct
    21001 gtaccacaag gcatcatcgg tctgatgacc cgcttcggta tggatgtcac cctggcccat
    21061 ccggaaggct acgacctgat cccggatgtc gttgaagttg ctaaaaacaa tgctaaagcc
    21121 tccggtggta gcttccgtca ggtcaccagc atggaagaag ccttcaaaga cgcagacatc
    21181 gtttatccga agtcatgggc accttacaaa gtgatggaag agcgtactga attgctgcgt
    21241 gcgaacgatc acgaaggctt aaaagcactg gaaaaacagt gtctggcaca gaacgcgcaa
    21301 cacaaagact ggcattgtac tgaagagatg atggaactga cccgtgatgg cgaagccctg
    21361 tacatgcact gcctgccagc tgatatcagc ggcgtatcct gcaaagaagg tgaagtgact
    21421 gaaggcgtat tcgaaaaata ccgtatcgct acctacaaag aagccagctg gaagccttat
    21481 atcatcgccg cgatgatcct gtcccgtaaa tacgccaaac caggtgcact gctcgagcaa
    21541 ctgctgaaag aagcgcaaga acgcgtgaaa taacccctcg ggccgatcag ctggtcggcc
    21601 ctctatttcc agaggccaaa aggataggat atgtccgttt tctcattgaa gattgatatc
    21661 gccgataaca aatttttcaa cggcgaaaca tcaccgcttt tttcgcaaag ccaggcaaaa
    21721 ctggcacgcc agttccacca gaaaattgct ggttatcacc caacaccgct ttgtgcgctg
    21781 gacgatctcg caaacctgtt tggtgtaaag aaaattctcg tcaaagacga atcaaaacga
    21841 ttcggtctga acgccttcaa aatgcttggc ggtgcgtacg ccatcgctca attattgtgt
    21901 gaaaaatatc atcttgatat cgaaacgctg tcatttgagc acctgaaaaa tgccatcggc
    21961 gaaaaaatga ctttcgcgac taccaccgac ggcaaccacg ggcgcggtgt ggcatgggca
    22021 gcacagcaac tcggacagaa tgcggtgatt tacatgccga aaggttctgc tcaggaacgc
    22081 gttgacgcca ttctaaacct cggtgccgag tgcatcgtca cggatatgaa ctatgacgat
    22141 accgttcgcc tgaccatgca acacgcgcag cagcacggct gggaagtggt acaggacacg
    22201 gcatgggaag gttacaccaa aatcccaacc tggatcatgc aaggctacgc aacactggcg
    22261 gatgaagccg tcgagcaaat gcgtgaaatg ggcgtcaccc cgacacacgt tctgctgcaa
    22321 gccggtgtcg gtgcaatggc cggtggtgtg ctgggttatc tggtcgacgt ctatagcccg
    22381 caaaatctgc acagcattat tgttgaacct gacaaagctg actgtattta tcgctccggc
    22441 gtcaaaggcg acatcgtcaa cgttggcggt gatatggcca ccatcatggc gggcctggcc
    22501 tgcggcgaac ctaacccact gggctgggaa atcctacgta actgcgccac ccaattcatc
    22561 tcctgccagg acagcgttgc cgcattaggt atgcgcgtgc tgggtaatcc gtatggcaac
    22621 gacccgcgca tcatctccgg tgaatccggc gctgtcggtt tgggcgttct cgcagcggtt
    22681 cattatcacc cgcaacgtca aagcctgatg gaaaaactgg cgctgaacaa agatgccgtg
    22741 gtgctggtta tcagcactga aggcgacacc gacgtgaagc actaccgcga agttgtctgg
    22801 gaaggcaaac acgctgtagc accttaatca ccctattgga actgctccct gcaaaacggg
    22861 gagtaaaaaa tctggagaaa aataatggct aagaatattc cattcaaact gattcttgaa
    22921 aaagcaaaag attaccaggc agacatgact cgcttcctgc gcgatatggt tgctattccc
    22981 agtgaaagct gcgacgagaa acgcgtagta catcgtatta aagaagagat ggaaaaagtc
    23041 ggcttcgata aagttgaaat cgacccgatg ggcaacgttc tcggttatat cggccacggc
    23101 ccgcgtctgg tggcaatgga cgctcatatc gataccgtcg gcattggcaa catcaaaaac
    23161 tgggacttcg atccgtacga aggcatggaa actgatgagc tgatcggtgg tcgcggtact
    23221 tccgaccagg aaggcggcat ggcatctatg gtttatgccg gtaaaatcat taaagacctc
    23281 ggtctggaag atgaatatac cctgctggtt accggtactg tgcaggaaga agactgcgac
    23341 ggcctgtgct ggcagtacat tattgaacaa tccggcattc gcccggaatt tgtggtcagt
    23401 accgaaccaa ccgactgcca ggtataccgt ggtcagcgcg gtcgtatgga aattcgtatt
    23461 gatgttcagg gtgtgagctg ccacggttct gcaccagaac gcggtgacaa cgccattttc
    23521 aaaatgggtc cgattcttgg cgaattacaa gaactctccc aacgtctggg ttatgacgaa
    23581 ttcctcggca aaggcaccct caccgtttct gaaatcttct tcacatcccc aagccgttgc
    23641 gctgtagcag atagctgcgc cgtctctatt gaccgccgtc tgacctgggg cgaaacctgg
    23701 gaaggcgcgc tggacgaaat ccgcgccctg cctgcagtac agaaagctaa cgcggttgtt
    23761 tctatgtaca actacgaccg tccgtcctgg actggcctgg tttacccaac cgaatgctac
    23821 ttcccgacct ggaaagtgga agaagatcac ttcaccgtta aagcactggt gaatgcctac
    23881 gaaggtctgt ttggcaaagc gccggttgtt gataagtgga ccttctcaac taacggcgta
    23941 tctatcatgg gccgtcacgg cattccggtg atcggctttg gcccgggtaa agaacctgaa
    24001 gcgcatgcac ctaacgaaaa aacctggaaa tctcacctgg tgacctgtgc cgcgatgtac
    24061 gctgcaatcc cgttaagctg gctggcaacc gaataattac tcttcactta ttcccctccg
    24121 gttcgccgga gggtttttgg agtttgctat gcgcgtattg atcaaaaacg gcactgtcgt
    24181 taacgcagat ggacaagcca aacaagattt gctgattgag agcgggattg ttcgccaatt
    24241 gggcagcaat atttcgccgc aggtcccgta tgaagaaatt gatgccactg gctgttacgt
    24301 tttccctggc ggcgtggatg tccatacgca tttcaatatt gatgtcggca tcgcgcgcag
    24361 ttgtgatgat ttttttaccg gtacccgcgc agctgcgtgt ggcggtacaa caaccattat
    24421 tgaccatatg ggatttggcc cgaatggctg ccggttacgc catcaactgg aagtttatcg
    24481 cggttatgcc gcccacaaag cagtcatcga ctacagcttt cacggtgtca ttcaacatat
    24541 caatcacgct atcctcgacg aaattccgat gatggttgag gaaggactga gcagttttaa
    24601 actctattta acctatcaat ataaactcaa cgatgatgaa gtgttgcagg cattacgccg
    24661 cttgcatgaa tccggcgcgc tgaccaccgt gcacccggaa aatgacgcag ctatcgccag
    24721 caagcgggcg gagtttatcg ccgcagggtt aaccgcgccg cgttatcatg ccttgagtcg
    24781 acctctggaa tgcgaagcgg aagccatcgc ccgcatgatt aacctggcac aaattgccgg
    24841 taacgccccg ctctatatcg tgcacctgtc taacggctta ggtctggatt atctgcgtct
    24901 tgcccgtgcg aatcaccagc cagtctgggt tgaaacctgc ccacaatatc tcctgttgga
    24961 cgaacgcagt tacgatacag aagatggcat gaagttcatt cttagcccac cgctgcgtaa
    25021 cgtacgcgag caggacaaac tgtggtgtgg catcagcgat ggtgcgattg acgtggtagc
    25081 gaccgatcac tgcacattct cgatggctca acgcctgcaa atttctaaag gcgatttcag
    25141 tcgctgccca aatggcttac ccggtgtgga aaaccgcatg cagttactgt tttccagtgg
    25201 cgttatgacg ggacgtataa caccggaacg ttttgttgaa ttaaccagcg cgatgcccgc
    25261 caggctgttt ggcctgtggc cgcaaaaagg attattagcg cccggttccg atggcgacgt
    25321 ggtgattatc gacccacgtc agagtcaaca aattcagcat cgccatctcc acgacaacgc
    25381 cgactactcg ccatgggagg gttttacctg tcagggcgcg attgtcagaa ctttatcccg
    25441 tggtgaaacg attttctgtg acggcacctt tacaggcaaa gccgggcgag gtcgtttcct
    25501 gcgacgcaaa ccgtttgtcc ctcccgtgct ctaaccactg ccagggatct atacacaatc
    25561 aagaatgatg agtgaggaaa aatgagtaag aaaattgttc tcgccctggg cgggaatgcg
    25621 ctgggcgatg acctggccgg acaaatgaaa gcggtaaaaa ttacttctca ggcaattgtt
    25681 gatttaattg cccagggaca tgaagttatc gtcactcacg gtaacgggcc acaggtgggc
    25741 atgattaacc aggcctttga agctgccgca aaaactgaag cgcactcgcc aatgctgccg
    25801 atgtctgtct gtgttgcctt aagccagggt tatattggtt acgatctaca aaacgcatta
    25861 agagaagaac tgctttctcg tggcattaat aaacctgtag caacgctggt tacccaggtc
    25921 gaagtcgatg ctaacgaccc ggcattcctc aacccgacca agccgatcgg ttcgttcttt
    25981 accgagcagg aagcagagca actgacaaaa cagggttaca ctctgaaaga agatgctggt
    26041 cgcggctatc gccgtgttgt tgcctcgccg aaaccggttg atattattga aaaagagacg
    26101 gtaaaagcgc tggtagaagc aggccaggta gtgatcaccg ttggcggtgg tggcattccc
    26161 gttattcgtg aagggaacca tctgcgcggt gccagtgcgg ttatcgataa agactgggcc
    26221 agcgcccgtt tagcagaaat gattgatgcc gatatgctga tcattctgac tgcggtagaa
    26281 aaagtggcca ttaacttcgg taaagagaat gaacagtggc tcgatcgcct gtcattaagt
    26341 gatgctgaac gctttatcga agaaggtcat tttgcgaaag gttctatgct gcctaaagtg
    26401 gaagctgccg cctcgtttgc tcgctctcgc gcaggtcgtg aagcactgat taccgtattg
    26461 agcaaagcga aagaagggat tgaaggcaaa accggaacgg tgatttgtca gtaatttaaa
    26521 ggccgggtgg gtaataccat ccggccattt tcatttaagc cacttccagc acttctttcg
    26581 tcgctttaac gccctgatgc atcaacatca ttaacgcctc aagtacgccg ccgccaatcg
    26641 cccgggcttt atcagaaacg ctggtgaaat cagccgtttc accacgagga tcgatatcac
    26701 caattttaaa accaccgacc actgccagtc cgtcgttcaa caagccacgc accatccccg
    26761 tcaacggtgc tttaatttca tgctcaccaa tccaggcaat cacatcgccc tctttcacca
    26821 gatcgcctaa tttcacgttg gatcgcataa tgcctgcagc aggagcacgg atcacccgtc
    26881 gggtggtatg ccccataata ttgccaggaa caccggtatt ctcctgcgca caaccagagt
    26941 aaatcacctg accgagccag tgcccgcgat ttgtttcaat taccgcatga caatccttcc
    27001 ctgcagtaaa gcccggccca agcgcgattg ttactggtgc catatctgcc cgcgtcccca
    27061 aattctgttt cgccagaata gcgtccacca cgcaaagcgg tttcagttca tcaagcaatg
    27121 aacaggcggg atctactatc acagggatga atccgcattc ggtaagtttc atcgcctccg
    27181 cagagctggt tgccaggcgg gcggtgacgc cttcgaccgt catttcgcca tcgaacacgg
    27241 cctgggcaaa tgccacggta caacgaatca ctgtcggttt ttccacttcc agcatgatca
    27301 ctttaaaacc cgcatgatac agacgtagcg ctacaccact ggcgatatcc cccgcaccac
    27361 gaatcaccac cagctggtgt ccggaaggat gagaaggctt catcatcagc ccacccggcg
    27421 cgttattttt cacctgtaat atttctgcca gcacgctgat ggcgatctcc tgcggcgttt
    27481 ccgcacctat gttgtaacca acgggcgcat gtaaacgggc aatatgttct tcagccacgc
    27541 ctttctcacg caattggcgc aggaaaagct gaaccttgcg gcgacttgcc agcaacccca
    27601 gccatgcaat gggttgttca atgagtttgt cgagggcttc acgatcctga ttattcgtgg
    27661 caatcaggac aaaattatca gggcgaatat ccagtgcttc cactgccgca ccaaacgact
    27721 cagcatggag aagcgtggtt gatggtggga atagttcagg attgaggctt tcgcgataaa
    27781 tatcggcaac ggcgatatca aatcctaata gcgccgcact ctgggctatc gcccggttga
    27841 catgccccgc gccaatcaac accagacgtg gacgcatacc atggacgctg ataaatactg
    27901 acattgcacc tccacaatct gacccgacag catccgcacc gttacgagcc atacgtccat
    27961 ggaataatcg cggcttacgt tcctgcaatg cctgaagcga ctcttcaatc accttccgct
    28021 caaccattcc gccaccaatt gtaccgacga tagagccatc ggcgcgcact aacatttgtg
    28081 cagaatggcg gggggttgag cctcggctat caacaatttg cgccatcgca aacggacaat
    28141 tttgctcttc gagttttgca gcctctgtga aaatattcat accaacctca atggatatcc
    28201 tttcagtaac ccggaatacc cgggccgcta attcacaaat ctgcgcgcga ttgcaggatg
    28261 ttcttgtata tcgcccagcc agattgcctc tacgtcgtgt tgttgcagcg gttgaaggag
    28321 ctcgctttgc gcaatcgcat tctcacattg agaaaaacgg ttaatgaacc agactcgccg
    28381 acaaccttgc ggtacgtttt taaacgcccc ctgagggtgg cgaaccagcg caacgagatc
    28441 gctcagttgc aaggttgcat caggtgttaa cccggtaata tcagcaaact gcgaccagcg
    28501 atggacattt tctgtgctca ctttcgcacc caaaatatgt cctcccatca cagcaatcac
    28561 gcagcaactg cttttaggta tgcaaggttc gtgctcatca ggcgctttta acggcattcc
    28621 acgcgagcca tctgcctcaa tgagaattac gtcacactct gatcgttgta ccagtgcatc
    28681 aatcgcttct ggcgtaaatc cctgcacttt tccctggttc gctttccagc tgtgaaaaca
    28741 aaatgaaatg ggggatataa aagacgcatg aggaagcatg gcgggatcac gacagaaaac
    28801 cacgggccag tgagatgttg gcataaacat atgtgtcgtc gtagtgatta atacacgcct
    28861 gccgctcgcc tggaacagtt ctgccagcca aaaaagcaag ctggttttcc ccccagcacc
    28921 aacaacagaa atcacagtgg gacgtttctg cgcgcctaaa tcaatgaata atgccgatgg
    28981 gtcaattata cttttcacgc gtctttatgg acctctctgg gataaattac cctcaactta
    29041 atccatcagg aagtaacgca attatcaggc gttattagct cctgtaaata atggaatcac
    29101 tatgtcagcc atcgactgta taattaccgc cgcaggatta tcatcaagaa tggggcaatg
    29161 gaaaattatg ttaccctggc aacagggaac aattcttgat acaagtatca aaaatgcgtt
    29221 gcagttttgt agccgaatta ttttagtcac cggctatcgt ggtaatgaac tgcacgaacg
    29281 ttatgcgaac cagagcaata tcactattat ccacaaccca gattatgcgc agggtttact
    29341 gacatcagta aaggccgcag tacccgcggt gcaaacagaa cattgttttc tcacccacgg
    29401 agatatgcca accctcacca tcgatatttt tagaaaaatc tggtcgttac gaaatgatgg
    29461 cgcaatactg ccactccata atggcatccc cggccatccg attttagtgt caaaaccatg
    29521 cctgatgcag gcaatccagc gacccaatgt caccaatatg cgtcaggcac tccttatggg
    29581 agaacattat tccgtcgaaa tagaaaatgc agaaataatt ttagatattg atactcctga
    29641 cgactttatt acagcgaaga aaaggtatac tgaaatttag aaaacgtagt taaacgattg
    29701 cgttcaaata tttaatccct ccagcactct ttatcataat gataaggaat cactgatttt
    29761 gagaaaagag aaaaagtgat aaatgggctg gcaataataa ttttcaggag tgattttaat
    29821 tctcatttgg cacctgtgag ttcattcaca aaaagccatt aagtttcgag ccgcctcaca
    29881 tttttttata tttcccgcca aacctggcaa gagtggtgcg attgttgctc tatcccccta
    29941 aaccaccgga tttctcaaca ccggtcactc aatgatatct gtataagcta aggagagggt
    30001 tatgggggat attatgcgtc ccattccgtt tgaggaactt ttgacgcgca tatttgatga
    30061 ataccaacaa caacgctcaa tctttggtat tcccgagcaa cagttttact cacccgtaaa
    30121 aggtaaaact gttagcgtct tcggtgaaac ctgtgccact cccgtcggcc ctgccgctgg
    30181 cccgcacacg cagctcgcgc aaaatattgt cacttcctgg ctgactggcg gacgcttcat
    30241 cgaactaaaa accgtccaaa ttcttgaccg cctggagctg gaaaagccct gtatcgatgc
    30301 cgaagacgag tgctttaaca ccgaatggtc taccgaattt accctgctta aagcctggga
    30361 tgaatacctc aaagcctggt ttgccctgca ccttctcgaa gcgatgttcc agccttctga
    30421 ttcctgtaaa tcgttcatct ttaatatgag cgtcggttac aacctcgaag gtattaagca
    30481 accgccgatg cagcagttca tcgacaatat gatggacgca tctgaccatc cgaaattcgc
    30541 tcaataccgc gatgcactta ataagctact ccaggatgac gcatttttag cccgccacgg
    30601 cttacaggaa aaacgcgaaa gcttgcaagc cttacccgct cgcatcccca ccagcatggt
    30661 acaaggggtt accctctcca ccatgcacgg ctgtcctccg catgaaatcg aagccatttg
    30721 ccgctacatg ctggaagaaa aagggctcaa cacctttgtg aaactcaacc cgaccttact
    30781 ggggtacgcg cgtgttcgtg agatcctcga tgtctgcggt ttcggttaca tcggcttaaa
    30841 agaagagtca tttgatcacg acctcaagct gacgcaagca ctggaaatgc tggaacgcct
    30901 gatggcactg gcaaaagaaa aatcactcgg ctttggcgta aaactgacta acactctcgg
    30961 caccattaat aacaaaggcg cactgcccgg tgaagagatg tatatgtcag gccgtgcgct
    31021 gttcccgctc tccatcaacg ttgcagcagt tctctctcgc gcctttgacg gcaaactgcc
    31081 catttcttat tccggtggtg ccagtcagct gactatccgc gatatttttg atactggtat
    31141 tcgccctatt actatggcaa ccgacctgct gaaacctggc ggctatctgc gcttaagtgc
    31201 ctgcatgcgc gagctggaag gctccgacgc ctggggactt gaccacgttg acgtcgaacg
    31261 actgaacaga ctggcagcag acgcgttaac catggaatac acccagaaac actggaagcc
    31321 agaagagcgt attgaagtgg ctgaagacct gccgctgacc gattgctacg ttgccccctg
    31381 tgttactgcc tgcgctatca agcaagatat tccggaatac atccgtctgc ttggcgaaca
    31441 ccgctatgcc gacgcgctgg aactcatcta tcaacgcaac gccctgcccg ccattaccgg
    31501 tcatatttgc gatcaccagt gccaatacaa ctgtacccgc ctggattacg acagtgcgct
    31561 gaatatccgc gaactgaaaa aagtcgcgct ggaaaaaggt tgggatgaat ataagcaacg
    31621 ctggcacaaa ccagccggtt ctggttcacg ccatccggtt gccgtgattg gtgcaggtcc
    31681 ggcgggtctg gccgcaggtt acttccttgc cagagcgggc catccggtta cgctgtttga
    31741 acgcgaagcc aacgcgggcg gcgtggtgaa aaatatcatt cctcagttcc gtattcctcc
    31801 agagttaatt cagcacgata tcgattttgt tgccgctcac ggcgtgaaat ttgagtatgg
    31861 ctgctcaccc gatctgaccg ttgaacagtt aaaaaatcag ggcttccact atgttctgat
    31921 tgccaccggc actgataaaa atagcggtgt gaaactggcg ggcgacaacc aaaatgtctg
    31981 gaaatcactc cccttcctgc gtgaatacaa caagggcaca gagctcaagc tgggcaaaca
    32041 tgtggtcgtt gtcggggcgg gtaacaccgc aatggactgc gctcgtgcgg cgttacgcgt
    32101 tccaggcgta gaaaaagcaa cggtcgttta ccgtcgttca ctgcaagaga tgccagcatg
    32161 gcgcgaagag tatgaagaag cgttgcacga cggcgtggag ttccgttttc tgaataatcc
    32221 ggaacgtttc gatgctgatg gcaccttaac cttgcgcgtt atgtcgcttg gcgaacctga
    32281 tgagaaaggt cgtcgccgtc cggttgaaac caacgaaaca gtaacactgc atgtagacag
    32341 cctgatcacc gccattggtg aacagcagga tactgaagcc ctgaatgcga tgggcgtgcc
    32401 gctggacaaa aacggctggc cagacgtcga ccataatggc gaaactcgtc tgactgacgt
    32461 ctttatgatc ggcgacgtac agcgcggacc atcctccatt gtcgctgctg tcggaaccgc
    32521 gcgtcgggcg accgatgcca tccttagtcg ggaaaatatc cgttcccacc agaacgataa
    32581 atactggaac aacgttaatc cggcggaaat ctatcaacgc aaaggcgata tctctgtcac
    32641 cctggtggat agcgacgatc gtgacgcgtt tgtcgcccag gaagccgcac gctgccttga
    32701 atgtaactac gtttgcagca agtgtgtgga tgtctgcccg aaccgcgcca acgtatccat
    32761 tgcggtccca ggcttccaga accgtttcca gacgctgcac ctcgacgctt actgtaacga
    32821 atgtggcaac tgcgcccaat tctgcccgtg gaacggtaaa ccgtacaaag acaaaatcac
    32881 cgtcttcagc ctgtcgcaag actttgataa cagcagcaac ccaggcttcc ttgtggaaga
    32941 ttgccgggta cgcgtacgtc tgaataacca aagctgggtg ttaaacatcg acaacgaagg
    33001 tcagttcaac aacgtaccac cggagctgaa cgatatgtgc cgcatcatta gccatgtcca
    33061 ccagcatcat cattatctgc tgggccgtgt ggaggtgtaa tcatgttgat tctgaagaat
    33121 gtcaccgcag tgcagttaca cccagcgaaa gtgcaggaag gcgttgatat cgccatcgaa
    33181 aatgatgtga ttgtcgctat cggcgatgcc ctgacgcaac gctaccccga tgccagctac
    33241 aaagagatgc atggtcggat tgtgatgcca ggaattgtct gctcgcacaa ccatttttac
    33301 tcggggctgt cccgcggaat tatggcaaac atcgccccct gcccggattt catctcaacg
    33361 ctgaaaaatc tctggtggcg actcgatcgc gcccttgatg aagagtcgct ctattacagc
    33421 ggactgattt gttccctgga agcgattaag ggcggatgta catcggttat cgatcaccat
    33481 gcctctccgg cgtatatcga cgggtcgctc tccacattgc gcaacgcatt tttaaaagtt
    33541 ggcctgcgcg cgatgacctg ttttgaaact actgaccgta acaacggtat caaagagttg
    33601 caggaaggtg tagaagaaaa catccgcttc gcccgtcaga ttgatgatgc gaagaaagcg
    33661 gcaactgaac cgtatctggt agaggctcat attggtgctc acgcgccgtt tactgtgccg
    33721 gatgccggtc tggaaatgct gcgtgaggcc gtgaaagcca caggccgtgg tttacatatt
    33781 cacgctgcgg aagaccttta cgatgtttcc catagtcatc actggtacgg caaagatctg
    33841 ctggcacgat tagcgcaatt cgatctcatc gacagcaaaa cgctggtcgc tcatgggctt
    33901 tatctgtcta aagacgacat cgcgttgctc aatcagcgtg atgcattcct ggtgcataac
    33961 gcccgttcaa acatgaacaa ccatgtcggc tacaaccatc atctttgcga catccgcaat
    34021 ctggcgttgg gtacggacgg tattggttca gatatgtttg aagagatgaa atttgccttc
    34081 tttaaacatc gcgatgcggg tggtccgctg tggcctgaca gctttgccaa agctctggct
    34141 aacggcaacg aactgatgag ccgcaacttt ggcgcgaaat ttggccttct ggaagccggt
    34201 tacaaagccg atttaaccat ttgcgattac aactcgccga cgccgctgct ggcagacaat
    34261 atcgccgggc atatcgcttt cggtatgggc tcaggcagcg ttcacagcgt gatggtcaat
    34321 ggtgtgatgg tctatgaaga ccgtcagttt aacttcgatt gcgattccat ttatgcgcaa
    34381 gccagaaaag ccgctgccag tatgtggcgt cggatggatg cgctggcata aataacagct
    34441 cccctcttct ttcagagaag aggggaaatt aaggaaagac gatgattgaa caatttttca
    34501 gacccgactc tgtcgaacag gcgctggaac tgaagcgccg ctaccaggat gaagccgtct
    34561 ggttcgccgg gggcagcaaa ctcaacgcta caccaacccg taccgataaa aagattgcca
    34621 tttccttaca ggatctggaa ctggactggg ttgactggga taacggtgca ctgcggattg
    34681 gcgcgatgtc tcgcttgcaa acactgcgtg atgcgcgatt tattcctgca gcactgcgtg
    34741 aagctctcgg ttttgtttac tcacgccatg ttcgtaatca gtcgaccatt ggtggtgaaa
    34801 ttgccgcccg gcaagaagag tcagtgctgc ttcccgtcct gctggcactg gatgctgaac
    34861 tggtttttgg caacggcgaa actctgtcaa ttgaggagta cctggcctgc ccatgcgatc
    34921 gcctgctaac cgaaatcatc attaaagatc cgtatcgcac ctgtgcgact cgcaaaatta
    34981 gccgttctca ggcaggttta accgtcgtga cggcagccgt tgcaataaca gaccacgacg
    35041 gtatgcgaat tgcgctggat ggcgtagcca gtaaagcact gcgtctgcac gatgttgaaa
    35101 aacaaaacct ggaaggtgat gcccttgaac aggctgtcgc caacgccatt ttcccgcagg
    35161 aagatttgcg gggcagcgtg gcctataaac gctatatcac gggagttctg gtagccgacc
    35221 tttatgccga ctgccaacag gctggggagg aagccgtatg atcatccact ttactttaaa
    35281 tggcacgccc caggagctaa ccgttaatcc tggtgaaaac gtgcaaaagc tgttgtttaa
    35341 catgggaatg cactctgtac gcaacagcga tgatggcttt gggtttgccg gttctgacgc
    35401 aattatcttt aacggtaata tcgttaacgc gtccttgctt atcgccgcac agttagagaa
    35461 ggcagatatt cgtaccgcag aatctctggg caaatggaat gagttaagtc tggttcaaca
    35521 ggccatggtt gatgttggcg tggtgcagtc tggttataac gatccagctg cggctctgat
    35581 tatcaccgat cttctcgatc gcatcgacgc acctacccgc gaagagatcg acgacgcgct
    35641 ctcaggtctg tttagccgcg atgctggctg gcagcaatac tatcaggtca ttgaactggc
    35701 ggttgcacgt aaaaataatc cgcaggccac cattgatatc gctccgactt tccgtgacga
    35761 ccttgaagtc attggcaagc attatcctaa aactgatgcc gcgaaaatgg tgcaggcgaa
    35821 accctgctat gttgaagatc gcgtaacggc tgacgcctgc gtcattaaaa tgttacgtag
    35881 cccacacgct cacgcactga ttactcatct ggatgtcagc aaagctgaag ccttaccggg
    35941 cgtcgttcac gttattactc acctgaattg cccggatatt tactataccc cgggtggtca
    36001 gagcgcaccg gaaccgtcac cgcttgaccg ccgaatgttc ggcaagaaaa tgcgtcacgt
    36061 cggcgatcgc gttgctgcgg tcgtcgctga aagtgaagac attgcgctcg aagcactgaa
    36121 gctcatcgac gttgaatatg aagtgcttaa gccggtaatg tcgattgacg aggcaatggc
    36181 ggaagatgcg cctgtcgtgc acgatgaacc ggtggtgtat gttgctggtg caccagatac
    36241 tctggaggaa gataactgtc atgcagccca gcgcggcgag catatgatca tcaacttccc
    36301 gatcggttct cgcccacgca aaaatatcgc tgccagtatt catggtcata ttggtgatat
    36361 ggacaaaggc tttgccgatg ccgatgtgat cattgagcga acctataact caacgcaggc
    36421 acagcagtgc ccgactgaaa cacatatctg ctttacccgt atggacggcg atcgtctggt
    36481 gatccacgcc tccacccagg taccatggca cttacgccgc caggtcgcgc gcctcgtggg
    36541 catgaaacaa cataaagttc atgtcattaa agagcgagtt ggcggcggtt ttgggtctaa
    36601 acaggacatc ctgctggaag aagtgtgcgc ctgggcaacc tgcgtgaccg ggcgtccggt
    36661 actgttccgc tacacccgtg aagaagagtt tattgctaac acctctcgtc acgttgcgaa
    36721 agtcaccgtc aaactgggag cgaaaagaga tggtcgcctg acagcagtga agatggattt
    36781 ccgcgccaac actggccctt acggcaacca ctcactcacc gtaccatgta acggaccggc
    36841 gctgtcgctg ccgttatatc cgtgcgataa cgtcgatttc caggtcacca cctactacag
    36901 caacatttgc ccaaatggtg cttatcaggg ttatggcgca ccgaaaggta acttcgctat
    36961 caccatggca ttagcggaac tggctgaaca gttacagatc gaccaactgg aaattatcga
    37021 acgtaaccgg gtacacgaag ggcaagagct gaaaattctc ggtgcaatcg gtgaaggtaa
    37081 agcgccgacc tccgttcctt ccgccgccag ctgcgcactg gaagagatcc tgcgtcaggg
    37141 tcgcgagatg atccaatggt cttcaccaaa accacaaaac ggtgactggc acatcggtcg
    37201 tggcgtcgcc attatcatgc agaaatcagg gatcccagat atcgatcagg ctaactgcat
    37261 gatcaaactg gaatcagacg gtacctttat cgttcattct ggcggtgcgg atattggtac
    37321 tggtctggat actgtggtga cgaaactggc agcagaagtg ctgcactgcc caccgcagga
    37381 cgtgcatgtt atctccggtg ataccgatca tgcgttgttt gataaaggcg catatgcctc
    37441 gtccggtact tgcttctcgg gtaacgcggc gcgtttggca gcagaaaatc tgcgggagaa
    37501 aattctgttc cacggtgcgc aaatgttggg tgagccagtg gcagatgtcc aactagcaac
    37561 gccgggcgtc gtacgcggca agaaaggcga agttagtttc ggagagatag cccataaagg
    37621 cgaaaccggc accggctttg gttcactggt gggaactggc agttatatca cgcctgattt
    37681 cgccttcccg tatggcgcaa acttcgctga agttgccgtc aacacgcgta cgggtgaaat
    37741 ccgcctggat aaattctacg ccttgctgga ctgcggtaca ccggtcaatc cagagttagc
    37801 gttgggacaa atctacggtg ccactctgcg agctatcggc cacagtatga gcgaagagat
    37861 catttatgac gccgaaggtc acccgttaac gcgtgattta cgcagttacg gcgcaccgaa
    37921 aattggtgac attccgcgtg atttccgcgc tgtgctggtg ccgagcgacg ataaagtcgg
    37981 cccattcggg gcgaaatcga tctcggaaat cggtgtaaat ggcgccgctc cggcgattgc
    38041 taccgcaatt cacgatgcct gcggcatctg gttacgcgaa tggcatttca caccggagaa
    38101 aatactcacc gcgctggaaa aaatataagt aataaatggc cgcttcggcg gcctgtttct
    38161 ctgtttgaac gatgactaaa tagtgactgc gaaaatgaga taagccgttc tcaaggcgtc
    38221 ctgcgcccca aattcgtggg aatttatttt actaattcag atgatcaaat ttactttaaa
    38281 aggagtgaag ggatgtctga tataaaccat gcaggttctg accttatatt tgaactggag
    38341 gatcgccctc cctttcatca ggctctcgta ggtgccatta cccatctgtt ggcaattttc
    38401 gttccgatgg taacccccgc gttaatcgtg ggtgcgacct tacagctttc cgctgaaaca
    38461 actgcctatc ttgtttctat ggcgatgatc gcctctggta ttggtacctg gttacaagta
    38521 aaccgctacg gcatcgtcgg ttctggtctt ctctcaattc agtcagtcaa tttttcattt
    38581 gttacggtca tgattgcgct gggcagcagc atgaaaagcg acggttttca cgaagagtta
    38641 atcatgtcgt cgctcctcgg cgtctccttc gttggcgcat ttctggttgt cggctcttct
    38701 tttatcctgc cctatttacg tcgggttatt acgcctaccg tcagcggtat tgtggtactg
    38761 atgatcggct taagcctgat taaagtcggc attatcgatt ttggtggagg atttgcagcc
    38821 aaaagcagcg gtacgttcgg caattacgaa catctcggcg ttggtttatt ggttttaatt
    38881 gtggtgatcg gctttaactg ctgtcgcagt ccgttgctac gcatgggagg gatcgccatt
    38941 gggctatgtg tcggctatat cgcatcgtta tgcctgggca tggtggattt cagcagtatg
    39001 cgcaatttgc cgttaatcac catcccgcat ccgttcaaat acggctttag ttttagcttc
    39061 catcagttcc tggtggttgg cacgatttat ctgcttagcg tgctggaagc agtcggcgat
    39121 atcaccgcca cggcaatggt ttcccgccgc cccattcagg gggaagagta tcagtcccga
    39181 ctgaaaggcg gcgtgctggc agatggtctg gtttctgtta tcgcctccgc tgtcggttca
    39241 ttaccattaa ccacgtttgc gcaaaataat ggggttattc agatgactgg cgtcgcttca
    39301 cgttatgtcg ggcgaaccat cgcggtaatg ctggttatcc tcggcttatt tccgatgatt
    39361 ggcggcttct tcacgaccat tccctcggca gttctgggag gcgcaatgac gttgatgttt
    39421 tccatgattg ccatcgctgg gattcgcatc atcatcacca acggattaaa gcgccgagaa
    39481 acacttattg tcgccacgtc tttaggttta gggcttggcg tctcctacga tcccgaaatt
    39541 tttaaaatat tgccagcctc tatttatgta ttagttgaaa accctatttg tgctggcggg
    39601 ttaactgcga ttttattaaa tattatcctc cctggtggct accgacagga aaacgttctg
    39661 cctggtatta cctcagcgga agagatggat taacagtaaa ggagtcaatg atgtcaggag
    39721 aacacacgtt aaaagcggta cgaggcagtt ttattgatgt cacccgtacg gtcgataacc
    39781 cggaagaaat tgcctctgcg ctgcggttta ttgaggatgg tttattactc attaaacagg
    39841 gaaaagtgga atggtttggc gaatgggaag acggaaagca tcaaattcct gacaccattc
    39901 gcgtgcgcga ctatcgcggc aaactgatag taccgggctt tgtcgataca catatccatt
    39961 atccgcaaag tgaaatggtg ggggcctatg gtgaacaatt gctggagtgg ttgaataaac
    40021 acaccttccc tactgaacgt cgttatgagg atttagagta cgcccgcgag atgtcggcgt
    40081 tcttcatcaa gcagctttta cgtaacggaa ccaccacggc gctggtgttt ggcactgttc
    40141 atccacaatc cgttgatgcg ctgtttgaag ccgccagtca tatcaatatg cgtatgattg
    40201 ccggtaaggt gatgatggac cgaaacgcac cggattatct gctcgacact gccgaaagca
    40261 gctatcacca aagcaaagaa ctgattgaac gctggcacaa aaatggtcgt ctgttgtatg
    40321 cgattacgcc acgcttcgcc cctacctcat ctcctgaaca gatggcgatg gcgcaacgcc
    40381 tgaaagaaga atatccggat acgtgggtac atacccatct ctgtgaaaac aaagatgaaa
    40441 ttgcctgggt caaatcgctt tatcctgacc atgatggtta tctggatgtt taccatcagt
    40501 acggtctgac cggtaaaaac tgtgtctttg ctcactgcgt ccatctcgaa gaaaaagagt
    40561 gggatcgtct cagcgaaacc aaatccagca ttgctttctg tccgacctcc aacctttacc
    40621 tcggcagcgg cttattcaac ttgaaaaaag catggcagaa gaaagtcaaa gtgggcatgg
    40681 gaacggatat cggtgccgga acaactttta acatgctgca aacgctgaac gaagcctaca
    40741 aggtattgca attacaaggc tatcgcctct cggcatatga agcgttttac ctggccacgc
    40801 tcggcggagc gaaatctctg ggccttgacg atttgattgg caacttttta cctggcaaag
    40861 aggctgattt cgtggtgatg gaacccactg ccactccgct acagcagctg cgctatgaca
    40921 actctgtttc tttagtcgac aaattgttcg tgatgatgac gttgggcgat gaccgttcga
    40981 tctaccgcac ctacgttgat ggtcgtctgg tgtacgaacg caactaataa taaaacttta
    41041 acatcctcgt gaggacatca ttatgtctgg agacatccta caaacaccgg acgcaccaaa
    41101 gccacagggc gcgctggata attattttaa aattaccgct cgtggcagta ccgttcgtca
    41161 ggaagtactg gctggcttaa cgacctttct ggccatggtt tattccgtta tcgtcgttcc
    41221 gggaatgctg ggcaaagcag gttttcctcc cgcagctgtg tttgttgcca cctgtctggt
    41281 cgcgggcttc ggctcgttgc tgatggggtt gtgggccaat ttgccaatgg cgattggttg
    41341 tgcgatttcc ttgacggcgt ttaccgcatt cagtctggta ctcgggcaac aaattagcgt
    41401 tcctgtcgca ctgggcgcgg tatttctgat gggcgtcatc ttcaccgcca tttccgtaac
    41461 cggtgtgcgt acctggatct tacgtaattt gccgatgggg attgctcacg gtacaggtat
    41521 cggtatcggc ctgtttctgc tgctgattgc tgctaacggt gtgggaatgg ttatcaaaaa
    41581 cccgattgaa ggcttgccag tggcgctcgg tgcgtttacc tccttcccgg tgatgatgag
    41641 cttgctgggg ctggcggtta tcttcggtct ggagaagtgt cgcgtacccg gcgggatctt
    41701 gttggtgatt attgcaattt cgatcatcgg cttaatcttt gacccagcgg tgaagtacca
    41761 cggtctggtg gcgatgccaa gcctgactgg cgaagatggt aagtctctga ttttcagcct
    41821 cgatattatg ggcgcactcc agccaactgt acttccgagt gtactggcat tggtgatgac
    41881 cgcagtgttc gacgccactg gcaccatccg tgccgtcgcc ggtcaggcga atttgttgga
    41941 taaagacaac cagatcatca acggcggcaa agccctgacc agtgactcag taagttcaat
    42001 attctccggc ctggtgggcg cagcgcccgc ggcggtttat atcgaatcag cggcaggaac
    42061 cgccgccggg gggaaaaccg gtttaaccgc aaccgtagtg ggggcgttat tcctgctgat
    42121 tctgttctta tcaccgctgt catttttgat ccctggttac gccactgccc ccgctctgat
    42181 gtacgtaggt ttgctgatgt taagtaacgt ctcgaagctg gatttcaatg attttattga
    42241 cgcaatggct ggcctggtgt gtgccgtgtt catcgttctg acttgtaata tcgttaccgg
    42301 tattatgctt ggctttgtga cactggtcgt aggccgcgtc tttgcacgcg aatggcaaaa
    42361 gctgaatatt ggtacggtga tcattactgc cgcactggtc gcattttacg cgggtggttg
    42421 ggcaatctaa tagatcctcc gcgcctttca gcgcggagat ttctttcaca gaggattctc
    42481 accgctggca acaatacgct gctggcggac tcgcctgagt tctctttcag taagcaactg
    42541 cagcgcctgt gttgggcaag attcaacgca ggctggcccc tcttcccgct gttcacagag
    42601 atcgcatttc acgatttgtt gccgggtatc ccccggcaac gactgaatgg tgatcattcc
    42661 aaacgggcac gcactgacgc aactctgaca gccaatacaa cgggcagaat tggtctgcac
    42721 cacctgctcg cccatcgtca gcgcccccac ggggcaagcg ctaacacaag gggcgttttc
    42781 acactgatgg cacatcaccg gcgcactgat gctgtccagt cgctgtacct tcagccgggg
    42841 taaaaagaca tcggcattca actcctgttc tgaagggtga gcgaccacac aggctacttc
    42901 acaagtacgg cagccaatac agtcagccgg attaacgata attaacgatt tcatcatgat
    42961 gccttcgtat caaacagagt taacatatcg cgcgccgcct gccttcctgc ggccattgca
    43021 gtgacaacca gatccgcgcc atgaacagca tcaccaccag caaagacttt tttcagatgc
    43081 gtctgggtag gtaaataccc gacgtcaccg gtttgaatca ggccccattt atcgagttta
    43141 attccgctgc cctgcaacca cggcatggca tgcgcctgga aaccaaaggc cataatgaga
    43201 acatcggcgg gcaattcaaa ctctgaaccc gctaccggac gaggacgacg gcgaccatcc
    43261 ggccccggct cacccatggc ggtacgaatc aggcccaccg cagttaaacg cccatcttcg
    43321 tcacaagcga tatattgcgg ctgaacattg aactgaaact cgacaccttc ctcgcgcgca
    43381 ttgaccacct ctttgcgcga gcccggcata ctgacttcat cacgacgata cgcgcaggtc
    43441 acgctggcgg cattgaggcg gatggaagtc cgcaaacaat ccatggtcgt atcgccaccg
    43501 cccaatacca cgactcgctt accttccaca tccgtcagcg gatactcttc agactccggc
    43561 aaccccatga gctggcgggt atgggcagtc aggaacggta acgcctgaat gacaccgggc
    43621 gcatcttcat gcggcagatc tgctcgcatc atcccgtaag tccccacgcc gataaaaact
    43681 gcatcatatt cagaagttaa atcgctaaag gtaatatcgc ggccaatttc acagttaaga
    43741 tgaaagtcga ttcccattgc ggtgaatatc tctcgccgct ggcttaatac cgttttatcg
    43801 agtttgaaag gaggtatgcc aaaagtcagc ataccgccaa tttctggatg gcgatcaaag
    43861 acatcaacct gaactcctgc gcgcgccaaa atatcagcac accctaaccc tgcaggccct
    43921 gcgccaatca ccgccacttt ttcgatacgg ggaacaactt tgctgacatc gggacgccag
    43981 cccatcgcca gcgcggtatc ggtaatgtag cgttccagat tcccgataga gactgcgcca
    44041 gagtgatctt tcaaagtaca tgcgccttca caaagacggt cctgtggaca taccctgccg
    44101 cagatttctg gcaaggaact ggtctggtgg caaagttctg ccgcttcaat aatctttcct
    44161 tcctgcacca gacggatgta atccggaata gcgttatgca gcgggcaatg ccagttgcag
    44221 ttagcttttt cggcgcaata aacacagcgg tcactctcat aagtcgcttg ttgtggatcc
    44281 agcccgtaat agatttcgcc aaagtgggtt ttccgttcac tcgctgaaat tttatctgcg
    44341 cctttacgcg agttaacggg gagcaacgct gcactgcgag atggctgagc gtctgatgac
    44401 gcttttcctg ctgccgtttt gcgctggcgg gccaccttta tctgctgcaa ccctttatcg
    44461 tccatcagcc gtaacgcctg tgttgggcag acttcaatac aggcttgcgt gccggaactg
    44521 cgctggttac aaaggtcgca tttctgcgca atcgtatcga ccatctcaac gacgccaaag
    44581 gggcaagcga ttgcgcatct tttacaacca atacattttt gctcgtccag ttgtacgcta
    44641 tcgggctgga aagtcagagc attaaccgga caagccgtaa cgcaaggggc attgttgcag
    44701 tgatggcagg ccaccggatt cgcagcctgg cctttcccta caacgtggat acgcggtcga
    44761 aagtcactgt gactcagcgg ccagttttct tgattatgtg ccatcgcaca ggcaatttca
    44821 caagcatgac agcctataca ttccgcagct tcagcagcga taaacttatt catttgcatc
    44881 cctttcattt aatgagttat gtcttctcaa atttcgcgat gcaataaatg ggcatgtatt
    44941 aggtagcatg gccgatttaa ttataatttt gcgactgagt tcaaattatt gcccctcaat
    45001 ctatgcagct ttcattattt caacattgcc gatcaaaaga atgctatcca gataaaaacg
    45061 attaaatgcg agagtgcgac atgccagaat gattaatgaa atatcaatat gagaaaatta
    45121 cagataattc cattgtcagg ttctcttctc attttcgtgg tttaaccacg attatgtgat
    45181 tacctccgca attcctgtct ctaactcccc ttcctcgcaa aactggcact ccacgagcat
    45241 gtatttagac agtttcatga acgtaaacgg ttgcttttta ctctggcggg cgaaaggaga
    45301 aacactgatg agcgccatag attcccaact tccctcatct tctgggcaag accgcccaac
    45361 tgatgaggtt gaccgcatat tatcaccagg aaagctgatc atactcggtc tgcaacacgt
    45421 ccttgtcatg tacgcaggtg cagtcgctgt tcctcttatg attggcgacc gactgggcct
    45481 ctcaaaagaa gctattgcga tgctcattag ctcggatctc ttttgctgcg ggatcgtcac
    45541 attattgcaa tgtatcggta tcggccgctt tatggggatc cgcctgccgg tgattatgtc
    45601 ggtgaccttc gccgctgtaa caccaatgat agccattggg atgaacccgg atatcggcct
    45661 gctggggata tttggtgcca ctatcgccgc gggttttatc accacattat tagcgccact
    45721 tatcggtcgc ttgatgcctt tattcccgcc actggttacc ggtgtggtta ttacttctat
    45781 cggacttagc atcattcagg tgggtattga ctgggccgca ggaggtaaag ggaatccgca
    45841 atatggtaat cccgtttatt taggtatctc ctttgccgtc ttaattttta tcttgctcat
    45901 tactcgctat gcgaaaggat ttatgtccaa cgtcgccgta ttactgggga ttgtatttgg
    45961 ctttttactt tcgtggatga tgaatgaagt caatttatcc gggctacatg atgcctcgtg
    46021 gtttgcgatt gtcaccccga tgtcgtttgg tatgccgatt tttgatcccg tttccattct
    46081 gaccatgact gccgtgttaa tcatcgtgtt tatcgagtcg atggggatgt tcctggcact
    46141 gggtgaaata gttggtcgta aactctcttc acacgatatt attcgcgggc tgcgtgtcga
    46201 tggcgtaggg acaatgatag gcggaacgtt taacagcttc ccccatacgt cattttctca
    46261 aaacgttggc ctggttagcg tgacgcgcgt tcatagccgc tgggtgtgta tttcttcggg
    46321 aattatatta atcctgtttg gcatggtgcc aaaaatggcg gtgctggtcg cctccattcc
    46381 gcaatttgtg ctgggcggcg ctggtctggt gatgttcggc atggtactgg cgacagggat
    46441 tcgaattctg tcgcgctgta actacaccac caaccgttac aacctctata ttgtggcgat
    46501 cagtctcggc gttggcatga ctccgacgct ctctcacgat ttcttttcta agttaccggc
    46561 cgtactgcaa ccgttgctgc atagcggcat tatgctcgca acccttagcg ccgttgtgct
    46621 gaacgtcttc tttaatggct atcagcatca tgctgatctg gtgaaggaat ccgtctctga
    46681 taaagattta aaagtcagga cagtacgtat gtggcttctg atgcgcaagc tgaagaaaaa
    46741 tgagcatgga gaataatatg aatcttttaa tgcgcgctat attcagtctg ctgttgcttt
    46801 ttactctctc tattcctgtc atttctgact gtgttgcaat ggccattgaa agtcgcttca
    46861 aatatatgat gctacttttt taaatggttt ttacctgtcg acatccgctc aaaacgggcg
    46921 gttgtcgata aacgctcact tggttaatca tttcactctt caattatcta taatgatgag
    46981 tgattagaat tacatgtgag aaattatgca aacggaacac gtcattttat tgaatgcaca
    47041 gggagttccc acgggtacgc tggaaaagta tgccgcacac acggcagaca ctctcttaca
    47101 tctcgcgttc tccagttggc tgtttaatgc caaagggcaa ttattagtta cccgccgcgc
    47161 ccttagcaaa aaagcgtggc ctggcgtgtg gactaactcg gtttgtgggc acccacaact
    47221 gggagaaagc aacgaagacg cggtgatccg ccgttgccgt tatgagcttg gcgtggaaat
    47281 tacgcctcct gaatctatct atcctgactt ctactatcgc gccaccgatc cgaatggcat
    47341 tgtggaaaat gaagtgtgtc cggtatttgc cgcacgcacc accagtgcgt tacagatcaa
    47401 cgatgatgaa gtgatggatt atcaatggtg tgatttagca gatgtattac acggtattga
    47461 tgccacgccg tgggcgttca gtccgtggat ggtgatgcag gcagccaata gtgaagcaag
    47521 aaaattgttg tctgctttcg cgcagcacaa ttaaaaaacc ccgacatttg ccggggttgt
    47581 gagcataacg taatgcttat ttcaccggac gcatcgccgg gaacagaata acgtcgcgga
    47641 tggtatgact gttggtgaac agcattacca tacggtcgat accaattccc agacctgctg
    47701 tcggcggtaa gccgtgttcc agtgcggtga cgtaatcttc gtcgtagaac atcgcttcgt
    47761 cgtcacctgc atctttcgcg gcaacctgat ccaggaagcg ctgcgcctga tcttccgcgt
    47821 cattcagctc gctaaagccg ttaccgattt cacgcccacc aatgaagaac tcaaagcggt
    47881 ctgtgatttc cgggttaacg tcgttacgac gcgccagagg agaaacttct gccggatatt
    47941 cagtaatgaa ggtcggctgg atcagatgtg cttctgccac ttcttcgaag atctcggtaa
    48001 cgatacggcc cagaccccag ctcttctcaa cgtggatgcc gatagattca gcaatcgctt
    48061 tcgcagagtc gaagttgtcc agatccgcca tgtcggtttc cgggcgatat ttcttgatcg
    48121 cttcacgcat ggtcagtttt tcgaacggtt taccgaagtc cagcgttacg tcgccgtagg
    48181 tcacttccgt cttaccgaga atatcctgtg ccagagtacg gaacagcgat tcggtcagct
    48241 cgatcagatc tttgtaatct gcgtaagcca tatagagttc catcatggtg aactctgggt
    48301 tatgacgtac ggaaatacct tcgttacgga agttacggtt gatttcgaat acacgctcga
    48361 agccaccgac caccagacgc ttgaggtaca gttccggcgc gatacgcagg tacatgtcga
    48421 gatccagcgc attatggtgg gtgatgaacg gacgcgcagc ggcaccgcca gggatcacct
    48481 gcatcatcgg cgtttcaact tccataaagc cgcggttcac catgaactgg cgaataccag
    48541 agaggatctg cgagcgcact ttaaaggtgt tgcgggattc atcgttagag atgagatcca
    48601 gataacgctg acgatagcgc gcttcctgat cctgcaagcc gtggaattta tccggcagcg
    48661 gacgcagtgc tttggtcagc agacgcagct cggtacagtg gatagacagt tcgccggttt
    48721 tggttttgaa cagtttacct ttcgcgccga ggatatcgcc gaggtcccat tttttgaact
    48781 gctcgttgta aacgccttcc ggcagatcgt cacgggcaac gtacagctga atgcggccac
    48841 ccacatcctg cagggtaacg aaagacgctt tacccataat acgacgggtc atcatgcggc
    48901 cagcaacggc aacttcgatg ttcagcgctt ccagttcttc gttctcttta ccgtcgaatt
    48961 ctgcgtgcaa ttggtcagag gtatgatcgc gacggaaatc gttcgggaag gcaatcccct
    49021 gctcgcgcag gttcgccagc ttctcacgac gcgttttcag ttcattgtta agatcgacta
    49081 ccgcgtcagc gccctgtgcg tgttgttcag acatgttggt tcctcataac cctgctttca
    49141 aacttgcttc gataaattga tccaggctgc cgtccagcac ggcctgcgtg ttgcgggttt
    49201 ctaccccggt gcgcagatct ttaatgcggg agtcatcaag gacataagaa cgaatctggc
    49261 tgccccagcc gatgtcggat ttattgtctt ccatcgcctg cttctcggca tttttcttct
    49321 gcatctccag ttcataaagc ttcgctttca tctgcttcat ggcctggtct ttgttcttgt
    49381 gctgggaacg gtcgttctgg cactgggtca cgatcccggt tgggatgtga gtaatacgca
    49441 ccgcagattc ggtacggtta acgtgctgac cgcccgcgcc ggacgcgcga taaacgtcga
    49501 tgcgcagatc cgccggattg atttcgatat cgatatcgtc atcaacttcc gggtaaacaa
    49561 acgcggagct gaacgacgtg tggcgacgac cgccggagtc aaacgggctc ttacgcacca
    49621 ggcggtgaac gccagtttct gtacgcagcc agccgtaagc gtaatcgccg gagattttga
    49681 tcgtcacgga tttaataccc gccacttcac cttccgactc ttcgatgatt tcagttttga
    49741 aaccacgcga ttctgcccag cgcagataca tacgctcaag catgctcgcc cagtcctgtg
    49801 cttccgtacc gccagacccc gcctgaatat cgaggtagca gtcggcgctg tcatattcgc
    49861 cagagaacat acggcggaac tcaagctgcg ccagtttttc ttccagagcg tcgagttcag
    49921 caacggcttc gttaaaggtt tcttcgtcgt cagcttctac agccagttcc agcagaccag
    49981 aaacatcttc cagcccctgt ttcatttggt cgagggtgtc gacaacggct tcgagggaag
    50041 aacgctcttt acccagggcc tgtgcgcgtt cgggttcgtt ccagacatcc ggctgttcca
    50101 gctcggcgtt tacttcttcc agacgctctt tcttggcgtc gtagtcaaag atacccccta
    50161 agaacgtcgg agcgttccgt gaggtcctga atgcgattat ttaccggatt aatttcaaac
    50221 atggtctgat ttcttttatt gagctagtca aaatgcggtg ataagagcgg gattgtaccc
    50281 aatccacgct cttttttata gagaagatga cgctaaattg gccagatatt gtcgatgata
    50341 atttgcaggc tgcggttgcc gcgaaactcg ttgatatcga gcttgtaagt cagttgcact
    50401 tcgcgcacgc cgttatccgg ccagagggcg gtatcgacat taaaagcaat accatccagc
    50461 agcggaccgc cgccgaccgg ctcgaccatg actttcaaat gacgctcgcc caccagccgc
    50521 tgttgcagca gacggaaatg accgtcaaac agcggctccg ggaacatctg cccccacggg
    50581 ccagcatcgc gcagcagctg cgccacttcc atagtcattt cggccgggct taacggaccg
    50641 tctgacacca cttcgccttg caatagtgaa ggatccagcc actcggtaac cagctcgcca
    50701 aaccgttgtt gaaagagttc gaatttatcc tcttccagcg acaaacccgc cgccatcgca
    50761 tggccgccaa acttgagcat catgccgggg tagagtgtgt ctaatcgctc cagcgcatca
    50821 cgcatatgca gcccctgaat ggagcgaccg gaacctttca gcgtaccgtc acctgctggc
    50881 gcaaacgcga taaccggacg gtgaaaacgc tctttgatgc gcgaagccag aataccgaca
    50941 acgccctgat gccattcggg gtggtacatt gccagcccgc cgggtaacgt gtcgcggctg
    51001 cgctccagtt tctcgcacag ggtcagggct tcaatctgca ttccttgttc gatctctttt
    51061 cgcgtctggt ttagcgcatc gagttcattt gccagcacgc gcgcttcgcc gatgttgtcg
    51121 cacagcaaca gcgccacacc gacggacata tcgtccagtc gcccggcagc attgagacgt
    51181 ggccccagcg caaaacctaa atcgctggcg gcgagtttta gtgcatcacg gtttgccact
    51241 tccagcagcg ctttaatccc tggacggcac tttccggcac ggatgcgact catcccctgc
    51301 caggtgagga tgcggttatt ggcgtccagc ggcacgacgt ccgccactgt tcccagcgcg
    51361 accagatcca gcagttctgc caggttagga attgcgacgc cgcgctcatc aaaccagccc
    51421 tgatcgcgca aaaaggtgcg cagcgccagc atcagataaa acgccacacc cacgcctgcc
    51481 agtgatttcg acgggaaatt acagtcgcgc aagttagggt taatgatcgc ttccgccgcg
    51541 ggtaatgttt cgcccggcaa atggtgatcg gtaacaataa ccggaatgcc caacgagcga
    51601 gcgtgttcaa cgcccgaatg ggaggaaata ccgttatcca ccgtgacaat taactgcgcg
    51661 ccacgggcat gggcctgatc gaccacttcc gggcttaagc cgtaaccgtc ttcgaaacgg
    51721 tttggaacca agtagtcgat attgctgcaa ccaagcgagc gcatcgccag cacgcttaga
    51781 gccgtgctgg tggcaccgtc ggcgtcgaaa tcaccgacca caataatccg cgtgccttcg
    51841 cgaaaagcgt tgtaaaggat ctcaacggcc ttttcgacgc cgctcagttg ctgccagggc
    51901 agcatgcctt taacactgcg ttccagttcc tgcgcgctac gcacaccccg gctggcatat
    51961 aaacggcgca gcaagggagg caattcagcg ggcaagtcgg ccgtttcatc gacttctcgg
    52021 cgacgaagtt gtatctgttg tttcacgcga attatttacc gctggtcatt ttttggtgtt
    52081 cgtcgaggaa ttctttcatg tctttcggcg gctggtaacc cggaacaagt gtgccattgc
    52141 tcagcacaac tgccggagta ccgctaacgc caagctggac gccaagtgcg taatggtcgg
    52201 caatatccac gtcgcagctg gctggtgcga cgcttttacc tgccatcaca tcatcaaacg
    52261 ctttgttttt atctttcgca caccagatag ctttcatttc tttctctgca tcgctgtcca
    52321 gcccctggcg cgggaaagca agataacgca cggtgatccc cagcgcgtta tagtctgcca
    52381 tttgctcatg cagtttgtgg cagtaaccac aggtaatatc agtaaacacg gtgatgacgt
    52441 gtttttcctg cggcgcttta taaacgatca tctctttttc aagcgcattc aactgcttta
    52501 acagcatctt attggtgaca ttgaccggag ccgtgccact aacgtcatac attggcccct
    52561 gaatgatatg tttaccatca tcggtgatgt acaacacacc gctgttagtc agaactgtct
    52621 tcatgccagc tacgggcgcg ggctgaatat cgctgctttt gatgcccatt ttggctaacg
    52681 tttgttgaat tgccgcgtca tcagcctgaa caaagcctga aaacgctgcc aacaaggtaa
    52741 ataacataaa acctttcttc ataaattttc ctgttctttt cagacatcac gcccgcgggt
    52801 gatgctgttg atgaagttgt cgcagacgct cggtagcgac atgcgtataa atttgcgtgg
    52861 tggagagatc gctgtggccc agtagcatct gcaccacgcg taaatccgca ccatgattta
    52921 ataagtgagt ggcaaaagcg tgacgcaaca catgcggtga cagcttttcg ctgtcgatac
    52981 ccgccagcac ggcataatgt ttaatgcggt gccagaaggt ctgtcgcgtc atctgctgcg
    53041 cacgctggct gggaaacaac acgtcaattg acacaccatt caacagccac ggacgcccat
    53101 gttccagata ggtttccagc cagtaaaccg cctcttcacc taacggcacc aggcgctctt
    53161 tgttgccttt accaatgacc cgcactacgc cctgacgcag gctgatatca ctcattgtca
    53221 gcccgaccag ttcagagaca cgcagtccgg tagcatacaa cacttcaagc atggctttat
    53281 cgcgtagctc cagtggctga tcaattaatg gtgcctgtaa taaacgttcg acctgcgctt
    53341 cgcttaaatc ttttggcaaa cgctggggca atttcggtga agcgagatgc gcactgggat
    53401 catcttcacg aaacttttcg cgataaagat actggaacaa tcggcgcact gcactcagca
    53461 aacgcgctga gctggtggct ttatacccgc cctccagccg ttctgccagt aatgcctgca
    53521 aatcgtcact ttgcgccgtc gccagcgtca acccacggtg atgcaaccac tccaccatca
    53581 ttgacagatc gcgacggtaa gcgttcaacg tattttcagc cagatttttt tccagccaca
    53641 gagcatcaag aaactgctcg atgcgtgcca gatcctgttt cacttgcgcc ccttatggtc
    53701 actcatttga tccattatgc cttattgtgc cgtgactaaa gcgattctga tacactagcc
    53761 gcaaaagcca cagcagaatc gagaagctta cgttatgaat atgggtcttt tttacggttc
    53821 cagcacctgt tacaccgaaa tggcggcaga aaaaatccgc gatattatcg gcccagaact
    53881 ggtgacctta cataacctca aggacgactc cccgaaatta atggagcagt acgatgtgct
    53941 cattctgggt atcccgacct gggattttgg cgaaatccag gaagactggg aagccgtctg
    54001 ggatcagctc gacgacctga acctcgaagg taaaattgtt gcgctgtatg ggcttggcga
    54061 tcaactggga tacggcgagt ggttcctcga tgcgctcggt atgctgcatg acaaactctc
    54121 gaccaaaggc gtgaagttcg tcggctactg gccaacggaa ggatatgaat ttaccagccc
    54181 gaaaccggtg attgctgacg ggcaactgtt cgtgggtctg gcgctggatg aaactaacca
    54241 gtatgacctt agcgacgagc gtattcagag ctggtgcgag caaatcctca acgaaatggc
    54301 agagcattac gcctgatgtc actgacggct tagcgcatat gctttgccgt catctttatc
    54361 tttgcgtctc ttgttgcaac aaaatccgcc gtaaatcccg ccattcagct tcgtccatgc
    54421 tgtcggctgc cagccataaa tgttgccgtt taccgctgtc agaacgtaaa cgcagcatca
    54481 tgccgctctt aatcatccac ggcgctttga cgatgctcca ctcctgccct tgccaacgca
    54541 aacgcccgtc catcaacagg cgaatttccc cctggcgagc attaatgcgg cgctggctgc
    54601 gaacgcaatc aaacaccacc agcgaaagca acaccatcca taacggggtg taactgagcg
    54661 gccagggcat gagtaaaata acagcggcaa ccagcccatg aatcagcaag gaaagccact
    54721 gtgcgcgcca ggagacgcgc aaatcagatt gccacaggac cacgttcccg gttccgtgtc
    54781 tggatgagtc ggaccatcat ttccagttct gcatcggccg gtttaccgtg attcatcagc
    54841 cagttaaaca ggtccgggtc gtcgcattcc agcagacgaa taaagatgcg tttttcgtca
    54901 tcgcttaagc tgtcgtactc atgttcgaaa aacggcatga ttgaaatatc gagttcgcgc
    54961 ataccacggc ggcatgccca atgaatgcgg gctttgttgt taatgtccat cttcttcctg
    55021 tctcacgaaa atccagtacc cggctattgt aacgtgtttt tcgacttctt ttgcgggaat
    55081 atcagtaaac acaatcgcga tcgcgaaatt aattcgcaac aattcaaggg cttcattttt
    55141 ttggaagtcg cctcgcaaag catatcagaa ggcacagatc gcgtagtgaa agcacttgca
    55201 ttgcctcata gctcttttac cattagtcat taatacgccg ttaagcaact caggactcta
    55261 ttatggcttt tacacctttt cctccccgtc agcctacggc ttctgcccgt ttaccgctga
    55321 cgctgatgac gcttgatgac tgggcgcttg ccaccattac tggcgcggac agcgaaaaat
    55381 atatgcaggg tcaggtgaca gcagatgtca gccagatgac agaagatcag cacctgctcg
    55441 ccgcccattg cgacgccaaa ggcaaaatgt ggagcaattt acgtctgttc cgcgacggcg
    55501 atggctttgc gtggattgaa cggcgcagcg tgcgtgaacc gcagctgact gaactgaaaa
    55561 aatatgcggt gttctctaaa gtgaccatcg cgccagacga cgagcgtgtg ctgcttggtg
    55621 ttgccggttt tcaggcgcgc gccgcgctgg caaatatctt tagtgaactg ccttcgaaag
    55681 aaaaacaggt agttaaagaa ggcgcgacca ctttgctatg gtttgaacac ccggcagaac
    55741 gtttcctgat cgtaaccgat gaagccactg ccaatatgct gaccgataaa ctgcgcggtg
    55801 aagcggaact gaacaatagc caacagtggc tggcattaaa cattgaagcg ggtttcccgg
    55861 tgattgatgc cgccaacagc gggcagttta tcccacaggc gaccaacctc caggcgctgg
    55921 gcggcattag ctttaaaaaa ggctgctata ccggacaaga gatggtggcg cgagctaaat
    55981 tccgtggtgc caacaaacgc gcgctctggt tgctgacggg tagcgccagc cgactgccgg
    56041 aagccggtga agacttagag ctgaaaatgg gcgagaactg gcgtcgtacc ggaaccgtgc
    56101 tggctgcggt aaaactggaa gatggtcagg tcgtggtaca ggtcgtcatg aataacgata
    56161 tggaaccgga cagcatcttc cgcgtgcgtg acgatgcgaa tacattgcat atcgagccgc
    56221 tgccgtattc actcgaagag taaatctctg tgtcgcatca tgcatttatc gcctgatgcg
    56281 acaagggtgt gtcttttcag gcctacaaag gcacacccat tacgcctgcc caatatataa
    56341 atagatcgcc agaaagtggc acacgctgcc gccgagcaca aagccgtgcc agatggcatg
    56401 gttgtaggga atgcgtttgc agacgtagaa aatcaccccg agcgagtaaa ccacgccgcc
    56461 tacagccagt aaggtaacgc tgcccgccgc aagcttaatt gccatttcat aaatcaccac
    56521 cagcgacagc cagcccatcg ccagataggt caccagagat aaaattttga atcggtgcgc
    56581 gatggtcagt ttgaacagaa tccccagcaa tgccaggctc cagataacaa tcatcaaccc
    56641 gcgcgctaac ggagaatcca gccccaccag caaaaacggc gtgtaggttc cggcaatcaa
    56701 taggtaaata gcgcaatggt caaatttctt cagccacatt tttgcccgtt gatgaggaat
    56761 ggcgtgatag agcgtcgaag cgaggaacag caggatcata ctgccgccat agaggctgta
    56821 actggttatc gctgtggcgc tggcattgag atccaccgcc tgaaccagta gcaacaccag
    56881 cccaacgata ccaaacacca acccaatgcc gtgactgacg ctgttggcaa tttcctctgc
    56941 cagcgaatat ccctgcttaa tgaggggctt ctgaaccata acttactccg gagaaacgta
    57001 cacgcacatg tatacctctc cagcgtaact gagaatagtt ccagtgaaca cctgttagct
    57061 aaaataaatt ctgatttgag actaatctcc gaaaaatcat gaaattaaat gcgaaatttc
    57121 aactaacagg cgtgagttca atttaaagac atttaaattc aatcacataa aactgtgtct
    57181 ggccgggata gatatcggca atgacctttt tcagatcagt cagggtcata ttttcctgct
    57241 ctgcatgttt ttccgtcagt gtatccagcg ttacggttga ggttgcggtg acttcaatcg
    57301 tgcaaaaata accgtcgtct tcaaaacgcc cgacacgaag cacatcaccc gttttgaagt
    57361 gcgattcaga ctcgtcgcgg atggtgatgg ttttacgccc agccagaatg tcatcctgga
    57421 aacgttgaaa aaaagtgatg tcgtttggct gcatggtact atttcctgta aaaattgact
    57481 catctggagc ctatgatagt gaaaaaactc accttaccga aagatttctt atggggcggc
    57541 gcagttgccg ctcatcaggt cgaaggcggc tggaacaaag gcggcaaagg gccgagcatt
    57601 tgtgacgttc tgaccggtgg cgcacacggc gtgccgcgcg aaatcaccaa agaagtgttg
    57661 ccaggaaaat actatccgaa ccatgaagcc gttgattttt atggtcatta caaggaagac
    57721 atcaagctat ttgccgaaat gggcttcaaa tgttttcgta catccatcgc ctggacgcgc
    57781 atttttccaa aaggcgatga agctcagcca aacgaagaag ggctgaagtt ctacgatgat
    57841 atgttcgatg aactgctgaa atacaacatc gaaccggtga tcaccctctc ccactttgaa
    57901 atgccgctgc atctggtgca gcaatacggt agctggacca accgtaaagt ggttgatttc
    57961 tttgtccgtt tcgcagaagt ggtatttgaa cgctataagc ataaagtcaa atactggatg
    58021 accttcaacg aaattaacaa ccagcgtaac tggcgtgcac cgctgttcgg ttactgctgc
    58081 tccggcgtgg tgtataccga gcatgaaaac ccggaagaga cgatgtacca ggtgctgcat
    58141 caccagtttg tcgccagcgc cctggcggtg aaagccgcgc gtcgcattaa cccggaaatg
    58201 aaagtcggct gtatgctggc gatggtgccg ctttaccctt actcctgtaa tccggacgat
    58261 gtgatgttcg cccaggagtc gatgcgcgaa cgctacgtct ttaccgatgt gcaactgcgt
    58321 ggctattacc cgtcctatgt gttgcacgag tgggagcgtc gcggatttaa catcaaaatg
    58381 gaagacggcg atctcgatgt gctgcgcgaa ggcacctgcg attatctcgg tttcagctat
    58441 tacatgacca acgcggtgaa ggccgaaggc ggcaccggcg atgcgatctc tggttttgaa
    58501 ggcagcgtac caaacccgta tgttaaagcg tctgactggg gctggcagat tgatcctgtg
    58561 ggtctgcgct atgcactttg cgaactgtat gagcgttatc agaagccgct gtttattgtc
    58621 gaaaacggtt ttggcgctta tgacaaagtg gaagaagatg gcagcatcaa cgatgactac
    58681 cgcattgact acctgcgcgc ccatattgaa gagatgaaaa aagcggtgac ttacgatggc
    58741 gtggatctga tgggctacac accgtggggc tgcatcgatt gcgtgtcgtt cactaccggg
    58801 cagtacagca aacgctacgg ctttatctat gtgaataaac atgacgacgg tactggcgat
    58861 atgtcgcgtt cacgtaagaa gagctttaac tggtacaaag aagtgattgc cagcaacggc
    58921 gagaagcttt aattacccga tgtgcctgat gcgccccgct catcaggcct ggcgctcctg
    58981 tcatatattg aactccagga gtttatattt acgcttcatc cgacacacaa agcgcacttt
    59041 attaacaagc taaaaaggcg ccgaagcgcc tttagaagat agtcgaatca gtgaattact
    59101 ggtattcgct aatcggtacg caggagcaga acaggttacg gtcgccgtaa acatcatcca
    59161 gacgtttcac tgtcggccag tatttgtctg ccacgcctgc cgggaatacc gcaacttcac
    59221 ggctgtacgg atgcgcccac tcggcgacca gttcgctctg aatgtgcggc gcgttcacca
    59281 gcgggttatc ttccagcggc cagacaccgg ctttcacctg gtcaatttct gcgcggatag
    59341 ccagcatcgc gtcgataaag cgatccagtt ccactttgct ttcagattca gtcggttcaa
    59401 ccatcagcgt acccgccacc gggaacgaca tggtcggcgc gtggaaaccg tagtcgatca
    59461 gacgcttggc aatatccagt tcgctgatgc cggtttcttc tttcagcggg cgaatatcga
    59521 gaatacattc gtgcgccacg cgaccgtcgc gaccggtata cagcaccggg aaggcatcct
    59581 gcaggcggct ggcaatatag ttggcgttaa gaattgccac ctggcttgct tttttcagcc
    59641 cttctgcgcc catcatgcgg atgtacatcc agctgattgg cagaatggat gcgctaccga
    59701 acggtgccgc agaaaccgcg ccctgacggg ttaacatgcc ttcaatttgt accacgctat
    59761 gacctggcac aaacggtgcc agatgcgctt tcacgccgat cggtcccata ccagggccgc
    59821 cgccaccgtg cggaatgcag aaagttttat gcaggttaag gtgcgagacg tccgcgccaa
    59881 taaagcccgg tgaggtgatg ccgacctggg cgttcatgtt cgcgccatca aggtaaacct
    59941 gaccgccgaa ctgatgcacg acttcacaca cttcacggat cgtttcttca tacacgccgt
    60001 gggtggaagg ataggtcacc atgatacagg agaggttatc gcccgcctgt tccgctttcg
    60061 cgcgcagatc agtcagatcg atgttgccgt ttttatcaca cgccacaacc accacctgca
    60121 ttcctgccat atgtgcagaa gcggggttag ttccgtgcgc agaagccggg atcaggcaga
    60181 tatcacgatg cccttcgttg cggctttcat gataatgacg aatcgccagc aggcccgcgt
    60241 attcgccctg tgcgccagag ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca
    60301 ccagccagtc agccagctgc gcaatcatct gctgataacc ttcggcctgc tccggcgggc
    60361 agaacgggtg cagttcggca aattccggcc aggtgattgg gatcatctcg gcggcggcgt
    60421 tcagtttcat ggtgcaggaa cccagcggga tcatcgcctg attcagcgcc agatctttac
    60481 gctccagcga gtgcatatag cgcatcattt cggtttcgct gtggtagcga ttaaacaccg
    60541 gatgggtgag gatttcgtcg tcgcgcagca tcgcaggctg aatagagcgg ctgtcgtgag
    60601 ccacgtcttt gtccagcgtg tcgatgtcca ggccgtggtt atcccccagc agtacgctga
    60661 aaagctgcat cacgttttca cgcgtggtgg tttcatcaag ggtgatccca accgcgttca
    60721 ggatatcgct acgcaggttg atttcagccg cttcggcacg cgccagtacg cccgccttgt
    60781 ctgccacttc cacacacaaa gtgtcgaaat agtgcgcatg acgcagtttc agaccttttt
    60841 gttgcaggcc cgccgccagg atatcggtca gacggtggat gcggttagcg atacgtttca
    60901 ggccaaccgg gccgtgataa acggcataca ggctggcgat gtttgccagc agtacctggg
    60961 aagtacaaat gttggagttc gctttctcac ggcgaatatg ttgctcgcga gtctgcatcg
    61021 ccatgcgcag agcggtattg ccagctgcat ctttcgatac accgataata cggcccggca
    61081 ttgagcgttt gtattcatct ttcgccgcaa agaatgctgc gtgtgggcca ccgtagccca
    61141 tcggcacacc gaagcgttgc gccgaaccaa agacaatatc cgcgccctgt ttacccggcg
    61201 cagttaacag caccagcgcc attatatcgg cggcgacact gaccacaatt ttgcgtgatt
    61261 tcagttcgct gatgagcgca gtgtagtcgt ggatttcacc ggttgtgcca acctgctgta
    61321 acagcacgcc gaagacgtcc tgatggtcga gcactttttg cgcgtcatcg acaatcactt
    61381 caaaaccaaa ggtttcagca cgagtacgga ccacatcgag cgtttgcgga tgaacgtcag
    61441 aagcaacgaa gaagcggttg gcatttttca gtttgctgac gcgtttcgcc atcgccattg
    61501 cttcggcggc agcagtggcc tcgtccagaa gagaggcaga ggccatatcc agcccggtca
    61561 aatccagcgt tacctgctgg aagttgagca gtgcttcaag gcggccctgg gagacttcag
    61621 gttgatacgg agtatacgcg gtataccagc ccggattttc cagcatgtta cgcagaataa
    61681 ccggcggtag ctgcacggcg gtgtaaccca tgccgatgta agacgtgaag cgtttattgc
    61741 gactggcaat agccttgagt tccgccagcg cggcgtattc ggtcgccggt gcgccaacct
    61801 gcggcggggt cgcaagctga atatctttcg gcacaatctg gccggtcagc gcgtttaacg
    61861 attgtgcgcc aacggcattc agcatttctt gctgttgcgc ggcgtccggt ccgatatggc
    61921 gttcaataaa agcgccgctg ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt
    61981 cctgaaacgt gcagtgaatt gtgaacctct ctccttgcga agagagtgag ggtgaggcgt
    62041 aaaagttcct caccctgatc ctctcccgca gaagaggaat aaagccgtta ctcgtcttct
    62101 aacaatgctt cgtatgcggt cgcatccagc agtgattcca gttcgctttc atcgctggct
    62161 ttgattttaa agatccagcc gcctgcatac ggttcgctgt tcaccagttc cggggaatcg
    62221 ctcagtgcgt cgtttaccgc cacgatttca ccgcttactg gcgcataaat gtctgacgcc
    62281 gcttttactg attcggcaac cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc
    62341 ggcaggtcaa caaacaccat atcgcctaac agctcctggg catgttcggt gataccaacg
    62401 gtgtaagtgc cgccggcttc tttacgcagc cattcgtgtt ctttgctgta tttcagttct
    62461 gctggtacgt tgctcatcaa tcaatctcca aaaaagtaaa tcacgcgacg gctttaccgt
    62521 tacgcacaaa aacaggtttc gtcactttaa ccggcatttc acggttgcga atttgtacaa
    62581 tcgctgtttc accaatacct tccggcacgc gcgccagcgc aatgctgtaa cccagcgtcg
    62641 gggagaaagt accgctggtg ataatgcctt catgctggtt gccctgcgca tcggtaaagc
    62701 gtaccggcag ttcattacgc agcacgcctt tttcggtcat caccagacca accagttttt
    62761 ctgtgccatg ctcacgctgc acttccaggg cttcacgacc gatgaagtca cgatctgccg
    62821 gttcccaggc gatagtccag cccatattgg cggctaaagg agagatggtt tcgtccatct
    62881 cctgactata aagattcata cccgcttcca gacgcagcgt gtcacgcgcg cccaggccgc
    62941 atggcttaac gcccgcttcc accagcgcgc gccagaaatc cgccgctttt tcattgggca
    63001 gcgcaatttc atagcccgct tcaccggtat aaccagtggt ggcaataaac agatcgcccg
    63061 cctgcacgcc aaagaacggt ttcatccctt ccaccgcctg acgctgggcg tcattaaaca
    63121 gtgtggcagc ttttgcctgc gcattcggcc cttgtacggc gatcatggaa aggtcatcac
    63181 gaacggtaat ttcgatgccg aaaggttcag cgtgttgggt aatccaggag aggtcttttt
    63241 cgcgggtggc ggagttaaca acgaggcgga agaaatcttc agtaaagtag tagacgatga
    63301 ggtcatctat cacaccgcca gaggcattca acatccccga gtaaagggct ttgccgcttt
    63361 tggtgagttt cgccacatcg ttcgccagca gataacgcag aaactcccga gtgcggctgc
    63421 cgcgaagatc gacgatggtc atatgtgaca catcaaacat tccggcatcg gtacgtaccg
    63481 catgatgttc gtcgatttgc gaaccgtaat gcagcggcat catccagccg tggaaatcca
    63541 ccatgcgagc gccgcaaagc gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt
    63601 cctcattgaa taagcggggc tgacaacttt tccatggcga aattatcacc acgaaaccca
    63661 gcatcggagc cactcccggt ccccaacgca atcgttctct tttgcctgaa cttaccaccg
    63721 aaacagactg ttaaccataa ggtaaaattg atcatcacat tagcttatgg ttaaaaaatg
    63781 caaaaatcgc aacagaataa aaaaccaaaa aacacatcag tttctataca aagatgatgt
    63841 gatgagaaag
//
LOCUS       DAZGHF010000011        62509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGHF010000011 DAZGHF010000000
VERSION     DAZGHF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.011"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..282)
                     /gene="chbB"
                     /locus_tag="ACHK3A_000771"
     CDS             complement(<1..282)
                     /gene="chbB"
                     /locus_tag="ACHK3A_000771"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO8738899.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLK"
     gene            complement(581..919)
                     /gene="osmE"
                     /locus_tag="ACHK3A_000772"
     CDS             complement(581..919)
                     /gene="osmE"
                     /locus_tag="ACHK3A_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO8738900.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1121..1948
                     /gene="nadE"
                     /locus_tag="ACHK3A_000773"
     CDS             1121..1948
                     /gene="nadE"
                     /locus_tag="ACHK3A_000773"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO8738901.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2178..3065
                     /gene="cho"
                     /locus_tag="ACHK3A_000774"
     CDS             2178..3065
                     /gene="cho"
                     /locus_tag="ACHK3A_000774"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO8738902.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3025..3600)
                     /gene="ves"
                     /locus_tag="ACHK3A_000775"
     CDS             complement(3025..3600)
                     /gene="ves"
                     /locus_tag="ACHK3A_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO8738903.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3803..4288)
                     /gene="spy"
                     /locus_tag="ACHK3A_000776"
     CDS             complement(3803..4288)
                     /gene="spy"
                     /locus_tag="ACHK3A_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO8738904.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTSAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4618..5586)
                     /gene="astE"
                     /locus_tag="ACHK3A_000777"
     CDS             complement(4618..5586)
                     /gene="astE"
                     /locus_tag="ACHK3A_000777"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO8738905.1"
                     /translation="MDNFLALTLTGKKTVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5579..6922)
                     /gene="astB"
                     /locus_tag="ACHK3A_000778"
     CDS             complement(5579..6922)
                     /gene="astB"
                     /locus_tag="ACHK3A_000778"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO8738906.1"
                     /translation="MNAWEINFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAS
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6919..8397)
                     /gene="astD"
                     /locus_tag="ACHK3A_000779"
     CDS             complement(6919..8397)
                     /gene="astD"
                     /locus_tag="ACHK3A_000779"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO8738907.1"
                     /translation="MTLWINGDWVTGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVMAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGRWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8394..9428)
                     /gene="astA"
                     /locus_tag="ACHK3A_000780"
     CDS             complement(8394..9428)
                     /gene="astA"
                     /locus_tag="ACHK3A_000780"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO8738908.1"
                     /translation="MMVIRPVERSDVAALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEESETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLAANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9425..10645)
                     /gene="astC"
                     /locus_tag="ACHK3A_000781"
     CDS             complement(9425..10645)
                     /gene="astC"
                     /locus_tag="ACHK3A_000781"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO8738909.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFAYRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRCGLFSEIRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSGGSS"
     gene            11091..11897
                     /gene="xthA"
                     /locus_tag="ACHK3A_000782"
     CDS             11091..11897
                     /gene="xthA"
                     /locus_tag="ACHK3A_000782"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO8738910.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTANRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12064..12774
                     /gene="ydjX"
                     /locus_tag="ACHK3A_000783"
     CDS             12064..12774
                     /gene="ydjX"
                     /locus_tag="ACHK3A_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO8738911.1"
                     /translation="MNAERKFLFACLIFALVIYAFHAFGLFELLTDLPHLLTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            12779..13456
                     /gene="ydjY"
                     /locus_tag="ACHK3A_000784"
     CDS             12779..13456
                     /gene="ydjY"
                     /locus_tag="ACHK3A_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO8738912.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13470..14177
                     /gene="ydjZ"
                     /locus_tag="ACHK3A_000785"
     CDS             13470..14177
                     /gene="ydjZ"
                     /locus_tag="ACHK3A_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO8738913.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14177..14725
                     /gene="ynjA"
                     /locus_tag="ACHK3A_000786"
     CDS             14177..14725
                     /gene="ynjA"
                     /locus_tag="ACHK3A_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO8738914.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SIFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLVTHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14735..15901
                     /gene="ynjB"
                     /locus_tag="ACHK3A_000787"
     CDS             14735..15901
                     /gene="ynjB"
                     /locus_tag="ACHK3A_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8738915.1"
                     /translation="MRHCVWLLGLLSLFSLATHASDWQEIKNDAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYDINLKIVHLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQFLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFSEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15874..17409
                     /gene="ynjC"
                     /locus_tag="ACHK3A_000788"
     CDS             15874..17409
                     /gene="ynjC"
                     /locus_tag="ACHK3A_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO8738916.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAVLTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQAKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLTATFIALILIL
                     AWLEWGSSRRHFWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGYLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRSKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17409..18062
                     /gene="ynjD"
                     /locus_tag="ACHK3A_000789"
     CDS             17409..18062
                     /gene="ynjD"
                     /locus_tag="ACHK3A_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO8738917.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGGLAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18129..19436
                     /gene="ynjE"
                     /locus_tag="ACHK3A_000790"
     CDS             18129..19436
                     /gene="ynjE"
                     /locus_tag="ACHK3A_000790"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO8738918.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSANWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKKRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDIEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19445..20065)
                     /gene="ynjF"
                     /locus_tag="ACHK3A_000791"
     CDS             complement(19445..20065)
                     /gene="ynjF"
                     /locus_tag="ACHK3A_000791"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO8738919.1"
                     /translation="MLDRHLHPRIKPLLHQCVKVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALIVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            20152..20559
                     /gene="nudG"
                     /locus_tag="ACHK3A_000792"
     CDS             20152..20559
                     /gene="nudG"
                     /locus_tag="ACHK3A_000792"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO8738920.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20525..20797)
                     /gene="ynjH"
                     /locus_tag="ACHK3A_000793"
     CDS             complement(20525..20797)
                     /gene="ynjH"
                     /locus_tag="ACHK3A_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
                     /protein_id="HFO8738921.1"
                     /translation="MSRALFAVVLAFPIIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQQDDKNLSTNPLVWRRVKP"
     gene            21033..22376
                     /gene="gdhA"
                     /locus_tag="ACHK3A_000794"
     CDS             21033..22376
                     /gene="gdhA"
                     /locus_tag="ACHK3A_000794"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO8738922.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPIDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(22453..24408)
                     /gene="topB"
                     /locus_tag="ACHK3A_000795"
     CDS             complement(22453..24408)
                     /gene="topB"
                     /locus_tag="ACHK3A_000795"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO8738923.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(24413..25456)
                     /gene="selD"
                     /locus_tag="ACHK3A_000796"
     CDS             complement(24413..25456)
                     /gene="selD"
                     /locus_tag="ACHK3A_000796"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO8738924.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRHACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRELLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(25573..26124)
                     /gene="ydjA"
                     /locus_tag="ACHK3A_000797"
     CDS             complement(25573..26124)
                     /gene="ydjA"
                     /locus_tag="ACHK3A_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO8738925.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAAMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            26285..28141
                     /gene="sppA"
                     /locus_tag="ACHK3A_000798"
     CDS             26285..28141
                     /gene="sppA"
                     /locus_tag="ACHK3A_000798"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO8738926.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            28306..29322
                     /gene="ansA"
                     /locus_tag="ACHK3A_000799"
     CDS             28306..29322
                     /gene="ansA"
                     /locus_tag="ACHK3A_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO8738927.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            29333..29974
                     /gene="pncA"
                     /locus_tag="ACHK3A_000800"
     CDS             29333..29974
                     /gene="pncA"
                     /locus_tag="ACHK3A_000800"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO8738928.1"
                     /translation="MHPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(30135..30407)
                     /gene="yeaC"
                     /locus_tag="ACHK3A_000801"
     CDS             complement(30135..30407)
                     /gene="yeaC"
                     /locus_tag="ACHK3A_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO8738929.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(30449..30862)
                     /gene="msrB"
                     /locus_tag="ACHK3A_000802"
     CDS             complement(30449..30862)
                     /gene="msrB"
                     /locus_tag="ACHK3A_000802"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO8738930.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            31204..32199
                     /gene="gapA"
                     /locus_tag="ACHK3A_000803"
     CDS             31204..32199
                     /gene="gapA"
                     /locus_tag="ACHK3A_000803"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO8738931.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            32283..33185
                     /gene="yeaD"
                     /locus_tag="ACHK3A_000804"
     CDS             32283..33185
                     /gene="yeaD"
                     /locus_tag="ACHK3A_000804"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO8738932.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGPAAQQGLPAHG
                     FARNLPWTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKACEIDLEAH
                     GEFETTSALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYL
                     NPQDCSVINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAY
                     ASETQKVTKEKPAHLAQSIRVAKR"
     gene            complement(33236..34090)
                     /gene="yeaE"
                     /locus_tag="ACHK3A_000805"
     CDS             complement(33236..34090)
                     /gene="yeaE"
                     /locus_tag="ACHK3A_000805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO8738933.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYAEGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQIPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(34180..34926)
                     /gene="mipA"
                     /locus_tag="ACHK3A_000806"
     CDS             complement(34180..34926)
                     /gene="mipA"
                     /locus_tag="ACHK3A_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO8738934.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYDGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            35362..37296
                     /gene="yeaG"
                     /locus_tag="ACHK3A_000807"
     CDS             35362..37296
                     /gene="yeaG"
                     /locus_tag="ACHK3A_000807"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO8738935.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            37409..38692
                     /gene="yeaH"
                     /locus_tag="ACHK3A_000808"
     CDS             37409..38692
                     /gene="yeaH"
                     /locus_tag="ACHK3A_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO8738936.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            38839..40314
                     /gene="cdgI"
                     /locus_tag="ACHK3A_000809"
     CDS             38839..40314
                     /gene="cdgI"
                     /locus_tag="ACHK3A_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO8738937.1"
                     /translation="MIRSTRISLGLFFNYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MASMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADQALREAKSEGGNKVIIHHI"
     gene            40495..41985
                     /gene="dgcJ"
                     /locus_tag="ACHK3A_000810"
     CDS             40495..41985
                     /gene="dgcJ"
                     /locus_tag="ACHK3A_000810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO8738938.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPDLRGTLQTASTDCDTIVEAAALLPAFDQAVESNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            42028..42531
                     /gene="yeaK"
                     /locus_tag="ACHK3A_000811"
     CDS             42028..42531
                     /gene="yeaK"
                     /locus_tag="ACHK3A_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO8738939.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(42532..42636)
                     /gene="yoaI"
                     /locus_tag="ACHK3A_000812"
     CDS             complement(42532..42636)
                     /gene="yoaI"
                     /locus_tag="ACHK3A_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO8738940.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            42806..43252
                     /gene="yeaL"
                     /locus_tag="ACHK3A_000813"
     CDS             42806..43252
                     /gene="yeaL"
                     /locus_tag="ACHK3A_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO8738941.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(43209..44030)
                     /gene="nimR"
                     /locus_tag="ACHK3A_000814"
     CDS             complement(43209..44030)
                     /gene="nimR"
                     /locus_tag="ACHK3A_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO8738942.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGVSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            44127..45308
                     /gene="nimT"
                     /locus_tag="ACHK3A_000815"
     CDS             44127..45308
                     /gene="nimT"
                     /locus_tag="ACHK3A_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO8738943.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSTMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVAAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            45363..45710
                     /gene="yeaO"
                     /locus_tag="ACHK3A_000816"
     CDS             45363..45710
                     /gene="yeaO"
                     /locus_tag="ACHK3A_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO8738944.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLANIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(45732..45986)
                     /gene="yoaF"
                     /locus_tag="ACHK3A_000817"
     CDS             complement(45732..45986)
                     /gene="yoaF"
                     /locus_tag="ACHK3A_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO8738945.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            46169..47194
                     /gene="dgcP"
                     /locus_tag="ACHK3A_000818"
     CDS             46169..47194
                     /gene="dgcP"
                     /locus_tag="ACHK3A_000818"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO8738946.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            47227..47325
                     /gene="yoaK"
                     /locus_tag="ACHK3A_000819"
     CDS             47227..47325
                     /gene="yoaK"
                     /locus_tag="ACHK3A_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO8738947.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            47328..47402
                     /gene="yoaJ"
                     /locus_tag="ACHK3A_000820"
     CDS             47328..47402
                     /gene="yoaJ"
                     /locus_tag="ACHK3A_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO8738948.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(47461..47709)
                     /gene="yeaQ"
                     /locus_tag="ACHK3A_000821"
     CDS             complement(47461..47709)
                     /gene="yeaQ"
                     /locus_tag="ACHK3A_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO8738949.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(47857..48039)
                     /gene="yoaG"
                     /locus_tag="ACHK3A_000822"
     CDS             complement(47857..48039)
                     /gene="yoaG"
                     /locus_tag="ACHK3A_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO8738950.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(48043..48402)
                     /gene="yeaR"
                     /locus_tag="ACHK3A_000823"
     CDS             complement(48043..48402)
                     /gene="yeaR"
                     /locus_tag="ACHK3A_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO8738951.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSTEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(48575..49213)
                     /gene="leuE"
                     /locus_tag="ACHK3A_000824"
     CDS             complement(48575..49213)
                     /gene="leuE"
                     /locus_tag="ACHK3A_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO8738952.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(49340..50263)
                     /gene="dmlR"
                     /locus_tag="ACHK3A_000825"
     CDS             complement(49340..50263)
                     /gene="dmlR"
                     /locus_tag="ACHK3A_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO8738953.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            50366..51451
                     /gene="dmlA"
                     /locus_tag="ACHK3A_000826"
     CDS             50366..51451
                     /gene="dmlA"
                     /locus_tag="ACHK3A_000826"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO8738954.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKKIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVID
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            51702..53312
                     /gene="yeaV"
                     /locus_tag="ACHK3A_000827"
     CDS             51702..53312
                     /gene="yeaV"
                     /locus_tag="ACHK3A_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO8738955.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHHV
                     EHYLPQTPVEVLEKTPVLPAGTVFKGDN"
     gene            53344..54468
                     /gene="yeaW"
                     /locus_tag="ACHK3A_000828"
     CDS             53344..54468
                     /gene="yeaW"
                     /locus_tag="ACHK3A_000828"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO8738956.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            54524..55489
                     /gene="yeaX"
                     /locus_tag="ACHK3A_000829"
     CDS             54524..55489
                     /gene="yeaX"
                     /locus_tag="ACHK3A_000829"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO8738957.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHLAELQYSDVDWQLHYCTRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLNIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(55536..56651)
                     /gene="rnd"
                     /locus_tag="ACHK3A_000830"
     CDS             complement(55536..56651)
                     /gene="rnd"
                     /locus_tag="ACHK3A_000830"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO8738958.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(56733..58418)
                     /gene="fadD"
                     /locus_tag="ACHK3A_000831"
     CDS             complement(56733..58418)
                     /gene="fadD"
                     /locus_tag="ACHK3A_000831"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO8738959.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDSEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(58623..59204)
                     /gene="yeaY"
                     /locus_tag="ACHK3A_000832"
     CDS             complement(58623..59204)
                     /gene="yeaY"
                     /locus_tag="ACHK3A_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO8738960.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(59243..59938)
                     /gene="tsaB"
                     /locus_tag="ACHK3A_000833"
     CDS             complement(59243..59938)
                     /gene="tsaB"
                     /locus_tag="ACHK3A_000833"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO8738961.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGADLPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDEKGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLTLCDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(59996..61906)
                     /gene="yoaA"
                     /locus_tag="ACHK3A_000834"
     CDS             complement(59996..61906)
                     /gene="yoaA"
                     /locus_tag="ACHK3A_000834"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO8738962.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            62038..62382
                     /gene="yoaB"
                     /locus_tag="ACHK3A_000835"
     CDS             62038..62382
                     /gene="yoaB"
                     /locus_tag="ACHK3A_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO8738963.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT        15196 a        16204 c        15512 g        15597 t
ORIGIN      
        1 cttaagcacg cctaaaccat cgactttgcc ataaagcagc gagtcaatta cttcgaccgg
       61 tttgttgggt aacaaacgct ggatttcggg caacatataa gcaatctgcg gccctaataa
      121 cacgacatcg gcattctgac ctttttcacc agccagtgtt tccggaaatg cttcaataat
      181 gaccggaact tcatattttt ctgcctgtgc gcgcattttt gataccagta aagaggtaga
      241 catgcccgca gaacaaaaca gataaatgtg tttcttttcc ataaaactgc cctcatcgac
      301 gattatctgt cagccagaca ctccgcaagc cttaacctgc ttccatgctc tgggtaactt
      361 gcgaaaccaa catgactttt tttgtggctg aaaggagtat acggtataga cctgagcaat
      421 agtatttctt tgacctgata aattgtctct ccttgacctg cggttatgac cctcttcaca
      481 tttcgggcaa ataattcgcg aagataatta agaactcagg ccaaaaaaaa ccggcgcaat
      541 ggccggtttc cgttgtaact caagctttca ggcgaagtga ttacttcgca gcctgtggat
      601 cagtgtcgta ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta
      661 acgcgacaaa gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag
      721 taccacgagc atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct
      781 gcgcccggct catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg
      841 tacgatcata agccgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc
      901 ctgccatatt cttgttcata ttccgtcctc ttgtttatca gcgtgttaga taagcctgga
      961 atacattggg cgctttttca agcccgtgaa cgaaacggct ccgctttcag aggattcctg
     1021 tatgacgttt taaccaccat tcagcccgct gtcgcttgtc gtttcagtag caacgggtta
     1081 gctttaagga agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat
     1141 aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt
     1201 cgattttctg aaaagctacc tgcaaactta tccgttcatt aaatcactgg tgctcgggat
     1261 cagcggcggt caggactcca cgcttgccgg aaagctgtgc cagatggcga ttaatgagct
     1321 gcgccaggaa actggcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt
     1381 tcaggctgac gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt
     1441 aaccgttaat atcaagggcg cggtattggc cagcgaacag gcgctgcgcg aagcgggtat
     1501 tgaattaagt gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata
     1561 tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat
     1621 taccggattc ttcactaaat atggtgacgg cggtacggac attaatccac tgtatcgtct
     1681 taacaaacgt cagggtaaac agttactggc ggcattaggt tgcccggaac acctttataa
     1741 gaaagcgcca acggccgatc tggaagatga tcgcccttct ctaccagatg aagtggcact
     1801 cggcgtgacc tatgacaata tcgacgacta tctggaaggg aaaaacgtac ctgaacaggt
     1861 cgccagaaca atagagaact ggtatctgaa aactgaacat aaacgccgtc cgccaattac
     1921 cgttttcgat gatttctgga agaagtaata atttgcacat attggtttgt gcgaaaaaga
     1981 gtactttgtt cacgccggat gcggcgtgaa cgccttattc gccccacaaa actatgcaaa
     2041 ttcaatatat tgcatgagtc gaataggcct gacaggcgta gcacgtctga cggtgtaacc
     2101 tttgtcatca acccgcctct tttttaatcg ctccccgcct gttacactgg atagataacc
     2161 agcattcgga gtcaactgtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg
     2221 cggcaattta tgaatatccc gaacatttac gttcattcct taatgactta cccacccgac
     2281 caggggtgta tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagca
     2341 ttaacattcg cagccgcgtc ctttctcatt tacgtacccc ggatgaagcc gccatgctac
     2401 ggcaatcccg acgcatcagc tggatatgta ccgccggtga aatcggcgct ctactccttg
     2461 aagcgcgatt aatcaaagag cagcagccgc tgtttaataa acgcttgcgc cgcaatcgcc
     2521 agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg
     2581 atttttcacg agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag
     2641 cattgcagac catcgctgat gaacaaaaac tttgttatgg tctgttggga ctggagccgt
     2701 taagtcgcgg tcgtgcatgt tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg
     2761 gtaaagagag ccatgacgat catgcgctac gtttgcgcca gtctctggaa cgtttacgag
     2821 tagtttgttg gccgtggaaa ggtgcggtgg cgctgaaaga acagcacccg gaaatgactc
     2881 aatatcatat tattcaaaac tggctgtggc tgggggcggt taattcgctg gaagaagcga
     2941 caatgttaat tcggacaccc gccgggtttg atcacgacgg ttataaaatt ctttgtaagc
     3001 cgctgctttc cggtaactat gaaattactg aacttgatcc ggcgaatgac cagcgagcca
     3061 gttgatttca ctgaacaaca gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc
     3121 atcgaaccag cacgcgccct ggtcggtagt gagcagttta tcgcccaatt gccacgcccc
     3181 gttaatgaca aacactaccc cgccacgtga gccaaaggta gtaaacgtac gttcggcaat
     3241 tctgactttc gccttacaga catccaggcg tgtcatgatg ttgaaatcca tcgacatctg
     3301 ccctgccgtc agtttcgctt ttaccacctg gtccgccgca aaggcaaaag gctgtaacgg
     3361 ttttaaggta tggttaaagc ggtctgtgct ttcaaggaac atctcgccgc cttccagcaa
     3421 cgtcactatc ctttccatac ctggaaacaa agaaaactcc ccattcgccg cgatggatgc
     3481 aatgctggca cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt
     3541 ttcaccggca gcatttcgcc acagattcac cgacatttta cgcatatcaa agtattccat
     3601 actcgctccc ggcccgttct tttcattgtt atcgttcagg taaccgtgga tggttatcgg
     3661 caactcgggt gatttacgtt agtggcgatc aggctttttt tgcatctgtt tgagaacaaa
     3721 aaagaaaacc gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt
     3781 cttacgtttt ttggctgaaa gattattcag cagttgcagg cattttacct tttgccgctg
     3841 gacgttctgt cagacgcttc tcaaaattag cattaaattg ctttttctgt tccggcgtca
     3901 agatgttgta aatcttgttc tgggtttcca tgtgcgccag catgttagct ttgcgctgtt
     3961 cttccatttt tgcgatctgc gcttcagctt ttgctttatc gaaggtatcg ctggcaatga
     4021 tgtcatgcat tgcgcggcgt tcttccagcg gcggacgttt catctggtca cgctggcctt
     4081 tcatgatttc gcggatctgc tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca
     4141 tgtcctgatg cggaccgaac ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg
     4201 ctgaggtagt ggtgtctgcg gcatgggcca ggttagccgc gccaagagcc agggtagagg
     4261 caacaaacag tgcagttaat ttacgcatat tctatatcct tcctttcagt tatttattac
     4321 ggctttctta agtagcgtgc cgtgttgacg agattaactt tactgacttt agcgtcaatt
     4381 aatcagagca acggtaaaac aatgaaagtg taaaaaacac ttttgcgcca attatggaga
     4441 aaaaaagaaa atttgatgga gagtaatgag agaatattac aacacgatga ttttgcagag
     4501 attatgaaga actataccgg atgactggtg ataaataaag caaataacca ggattaatct
     4561 gtattaattt ataagaaagt aacttaatac ccgcagaatg atttctgcgg gtaagtatta
     4621 gcttattttt tcgagcatta atcccgcgcg taatcccaac gctaccaaag gattagggaa
     4681 taacacatac tctacatcat gggttacggt aaaacgttcc tccccgtcct gcgccagcaa
     4741 tgttcctttc tcaaacggca taaaattcag cgtgtcactt gccatatgca tttcgaagga
     4801 cggcgagtgg cgagtaattt gcgaaaccac ccgataacgg agcggcggcg ttctcacgat
     4861 accgacactc tcaccagata gcagcgcagc aattgcgctg gcagtgactg caaactggcg
     4921 aagatcgttt tgtccaaagg gcaacgcttt gccgagttcc agcgtacagg ccagcgcgcc
     4981 aaaatgtctg gcgctgaaat gggtaaacgt accaccaggc tcctgatgga acaccagtgc
     5041 ctccagaccc gccgcaccca gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg
     5101 cggtaataca ccgaaccgcg gatgcaagga gccacgaatt gcggtatgga gatcaaggtg
     5161 ccagcgcaca gattctttgc cctggtcata aaaatcttcc aggcactgct ccagttcgcg
     5221 cgcccgacag gtttccccgc tttcagcaaa tagctgccaa cgaccgccaa acattcgatt
     5281 catatcgcta tggcaataac gtttcccttg cttcagcgca ggaggattcc cgaggatcac
     5341 cagtaaccgc caacgtaacg ggatctcacc gtgagatatc gcgccaagca acgcgtccag
     5401 catttccaca ggtgccgtct cattaccgtg gatccccgct gaaatcacca gcgcgccttg
     5461 cggtggcgtt aatggcgtca gttccagcac gccatcgccc agccagcgcc agcgaacgcc
     5521 gttgatttct cgctcggtga taaccgtttt tttacccgtt aaggtcagag caagaaaatt
     5581 atccatttcc ccctccctcg cgctggaacg gataaaccga accgagattc agtaattggc
     5641 tcaatacatc cagtgcttcc cgcccttcgc gcagcaattg cgggtcggcc agatcggcag
     5701 cagtaaggcg atcgcggtag taacgatcca cccagtcatt gagcgcatta aacagcgtat
     5761 cgttcatcat caccgccgga ttcactgccc ggcgttcttc ttctgtcaat accacgcgca
     5821 accgcaggca cgccgggcct ccgccattcg ccatgctttc acgtaaatca aagactttta
     5881 gttcgctaat cgggttgtca gcggcaagga gttcattgag ataaccccat actccggcgt
     5941 gttcccgaca ctcctgaggc agcaccaaca tcatggaacc atcatcgcgg ctcagcagtt
     6001 ggctgttaaa cagatatgta gacaccgcat ctgacacgga aacctgagtc gccggaactt
     6061 ctatcgccat aaaaccgttc acccgcgcac gcaggcttgc cagtaactgt gactggcgag
     6121 cgaacgcctg ttgatggcaa aacagcacct ggcggttact cacggcaatc acgtcattat
     6181 gaaaaacgcc ctggtcgata acgtccgggt tttgctgggc gaaaatcacc tgttggggat
     6241 tcacctgatt cagccttgcc accgcctcgc tggcttcgcg agtctgccgc gccggataac
     6301 gggaaggccg ggtatcattg ccttcttctc gcccgtagac aaaaagttgc atacccggtt
     6361 caccgtaatg accgccgaga cgattgtggt ttgccgcccc ctcatcaccg agcaacgcta
     6421 cctgcggcaa cgccgaatgg acactaaatt tctcttcgtc gttaaaaatc gcttttaaca
     6481 acgattcggt gacgggggct tccagcgaac ggtgaaattt attgttcagg ttggcaacgg
     6541 tgagatgcac tttgccatcc agcgtatcgg cagatggcgc gatcgttgcc gcattggcta
     6601 cccacattgg cgaagcggag ctgacgctgg aaagccagtg cggtgcctgg cgggcaacct
     6661 tttccagtac ctgctcatcg ctgccgctga atcccaactg acgcagcacc ggaataaacg
     6721 gacgctcgtg cggcgggatc accgcctggg ggaatcccgc gtcggcaagg gctttcattt
     6781 tcagtaagcc ctgcttcgcc gccagtcgcg ggttagagac ctgaaaacgg tgacgggtgg
     6841 aggcttcatt accaaacgac aggcccgcgt aatgatgcgt cagccctacc agcccgtcga
     6901 aattgatttc ccaggcgttc atcgcaccac ctcatcggaa aaatccagcc cggggttaag
     6961 cgttgcgggc aatgttaacg agtccgactc cagacttgcc atcggccagg cacaataatc
     7021 tgcggcatac caggcgctgg ggcgatggtt accggaagcg ccaatgccgc cgaatggcgc
     7081 ggtactggca gcaccggtaa gcggtttgtt ccagttaaca atccccgccc gtgcctccag
     7141 caacagttga tcgaattttt cccgctcggg ggaaaccaga ccgcaagaga ggccgaagcg
     7201 agtgttattc gccattcgaa tcgcttcatc gaaattgtca taacgccaga cgcgcaataa
     7261 cggcccaaac acttcttcat ccggcacgcc agtaacgcct gtcatttcaa tgatccccgg
     7321 cgtcagcaac gatgtccctg cttgtaataa gcgcggcgca agcagggtcc gtccgcccat
     7381 cgcctccagt tcctgccagg cagtaaccac ctgctgtgcg gcctgctcag aaatcagccc
     7441 gccaataaac ggctgcggtt cgtcatccca acggcccggc gttaaccgct ggctgacggc
     7501 aaccaggcga gcaagaaatg catcgccctg cgttccactt ttcagcaata aacggcgggc
     7561 gcaggtgcag cgttgcccgg cagtgacaaa cgccgactga atggtcagat ggacagcggc
     7621 atcgatatcc gccacctcat cgataattag cgggttatta ccgcccatct caagggcgag
     7681 aattttctcc ggctgaccgg aaagctggcg atgcagctgg tagccggtat tggcgctgcc
     7741 ggtaaacagc aagccgtcga gatcctccag cgcactcagc gcctgacccg tttcacgccc
     7801 gccctgcacc aggttcagca cgcccggcgg caagccagcc tgctgccata aacgcattac
     7861 cgcttcgcca ctccacggtg tcagttcgct gggtttaaag ataatggtgt tacctgccag
     7921 caatgccgga acgatatgtc cgttcggtaa atgaccgggg aaattatacg gcccaaacac
     7981 cgccagcacg ccgtgcgggc gatgtcgcag gctcgccgcg ccgtccggca tttcactacg
     8041 ctgctcgccg gtacgaacgt gatacgcctt aattgatatc gcgattttat tgatcatcgc
     8101 catcacttcg gttgccgctt cccagcgcgg cttacccgtt tctctggcaa taatcgcggt
     8161 taattcggct ttattgcttt ccagcaatcc ggcaaagcgt tcaactctta cctgacgatc
     8221 gccaaaggag agacgcgccc agcgcggaaa cgccgcacgg gcagcccgac aagcctgccc
     8281 gacctgagcg gcatcggcat cattgccttg ccataacacc tcgcccgata ccggattacg
     8341 cttcacacgc gatgcgccct ggcccgtaac ccagtcacca ttgatccata aagtcatgct
     8401 gttttctcct ctgcgcacag gcgcaccaga cgaacgcgat ccccggcgtg gcatttcagg
     8461 gcatccagtt gtgcggcggt taatatcaaa cgctcggttg ccggatcggt acgcaccagc
     8521 accacgcgga aatggtgata attttcattg gcggccaggc aggccgggaa atcgccctgc
     8581 gcaggctgcc cttctgccac ttccaccagc cgacttttgc ggatggcgcg cacgcggtcg
     8641 atgtcacact caagcgtcgg cccgccgtca aagatgtcga tatagttgcg gtagcgaaaa
     8701 ccttctttct ccagcaccgc gcgggcgggc gcggtttgcg gatgtacctg gccgataacg
     8761 tcctgcgctt cctgggataa aaagtgggta tagatcggat gtttcggcat cagttccgca
     8821 ataaatgcct tttgcccggt gccacagaga aaatcggcgc ggctaaaatc catcgaaaag
     8881 aagcgtttac cgaggctttg ccagaacggc gaatagccat gttcgtcaat caccccgcgc
     8941 atttcggcga ccactttgtc attaaacttg tcgcgaaagg ccgccataaa cataaagcgc
     9001 gatttcgaca gcaaatagcc gttgccctct ttgcgccagt ccgggtcgag aaacagcgtg
     9061 cacagctcgc tgctgccggt gtgatcgtta ctgagaaaca gcgtcggcaa cgcgttatag
     9121 acattcagct cttttgaggc gtgaaccagc gtgccgacgc gatagttgta ccagggatcg
     9181 ttcagcccaa ccgccacctc aatggcgcaa atccccgcca cagtgcctgt ttcgctctct
     9241 tccagtacaa acacataacc ctgctcgctt ttgggcagtt cgccctgcca ggttttgatg
     9301 gccctttcga tacgcgctga aagcgtggct tcattagcag ggagcgacgt caggccgccg
     9361 cccgttttgc tggcaagctg catcagcgcc gcaacgtctg agcgctcaac gggacggatg
     9421 accatcatga tgaacctccg ctcacaaagt gttcacaagc cgccgcaaaa cgatccagcc
     9481 cggtcgtcac ctcttcttcg ctgacattga gcgctggcgc aaaacgcact acgttgccac
     9541 cggcaatcag caccattacg cccgctttcg ccgcttcctg agaaatttgt ttcgcttgcc
     9601 cggcgtaatc ggcattcagc acacagccaa tcagcagacc taatccgcga atttcactga
     9661 acaaaccgca gcggtgatta atggtattaa gacgctcaac aaaccagtcg tgacgctgtt
     9721 taacgccatt aagcatctct ggcgtgttga tgagatccag cactttacct gccaccgccg
     9781 aggccagcgg attaccgcca taagtggtac catgtgtgcc cacggtcatc acgctggcgc
     9841 attcttcggt tgccaacaga gcgccaacgg ggaaaccgcc gcccagtgct ttggcggtag
     9901 ttaacagatc gggcgtcacg ccgtagtgca tataggcata cagttccccg gtgcgcccga
     9961 cgccggtttg tacttcatca aaaatcaaca gcgcattgtg gcggtcacac aattcacgca
    10021 gaccttgtaa aaatgcgttg cttgctggca ccacaccgcc ttccccctgg atgggttcga
    10081 caatcaccgc acaggtagag tcgtcaatca gcgcgctggc agagttaata tcgttatatg
    10141 cggcatgacg aatatccggc ggcagtggcg caaaatcctg tgaataggct ggctgaccgc
    10201 ccgcactgac ggtaaacagc gtgcgaccat gaaacgcatt tttaaacgcc acgataccgc
    10261 tcttatggct gccgtagcgg tcgtgagcga atttacgcgc cagttttagc gccgcttcgt
    10321 tggcttccgc accggagtta caaaagaaga cgcgataggc aaacgtggcg tcgatcaatt
    10381 ttttcgccag tcgcagtacc ggctcgttgg tgtaaccgtt gccggtatgc cagaacttac
    10441 tcgcctgttc gttcagcgcc tcacgcagtt ccggatgcgc atggcccagc gcgttcaccg
    10501 caatgccacc cgcgaagtcg atatactctt tcccctgctg atcccacaag cgcgaacctt
    10561 cgccacgtac cggtataaac ggtgccggag cgtaaacagg tatcatccat tcatcaaagt
    10621 tttcacgcgt aattggctga gacatagcga cctctacagt aaataattcg ttatttatat
    10681 gttaataata agtaatgttt gcgctgtaaa tgtagattgc agggttcgtg ccagccagtg
    10741 ataaaagtgc ataaacggcg gaggctaact ggaaatcaag gagttataac caaaccatat
    10801 gcatttaaag tgcatataaa gtgaatacgt ttgcgatgtg ggtgaataaa aagaataaaa
    10861 aacgcaatgt tatgcagaag taaaatataa ttctggaatt gtgatcattg acgaaattta
    10921 ctggaaatta ctgcgccatt ctgacgcaac gcgcaccaaa agcgggcatt ttttgcgcca
    10981 tcgttgacat cattaacaac catcgatcaa atcacttaac aacaggcggt acgcaaagcg
    11041 aaattctgct accatccacg cactctttat ctgaataaat ggcagcgact atgaaatttg
    11101 tctcttttaa tatcaacggc ctgcgcgcca gacctcacca gcttgaagcc atcgtcgaaa
    11161 agcaccaacc ggatgtgatt ggcctgcagg agacaaaagt tcatgacgat atgtttccgc
    11221 tcgaagaggt ggcgaagctc ggctacaacg tgttttatca cggacagaaa ggccattatg
    11281 gcgtggcgct gctgaccaaa gagacgccga ttgccgtgcg tcgcggcttt cccggtgacg
    11341 acgaagaggc gcaacggcgg attattatgg cggaaatccc ctcgccgctg ggcaatgtca
    11401 ccgtgatcaa cggttacttc ccgcagggtg aaagccgcga ccatccgata aaattcccgg
    11461 caaaggcgca gttttatcag aatctgcaaa actatctgga aaccgaactc aagcgtgaaa
    11521 atccggtgct gattatgggc gatatgaata tcagtcctgg cgacctggat attggcattg
    11581 gcgaagagaa ccgtaagcgc tggctgcgta ccggaaaatg ttctttcctt ccggaggaac
    11641 gcgaatggat ggagagactg atgagctggg gattggttga taccttccgc catgcgaatc
    11701 cgcaaacagc caatcgtttc tcatggtttg attaccgctc aaaaggtttt gacgataacc
    11761 gtggtctgcg catcgacctg cttctcgcca gccaaccgct ggcagaatgt tgcgtagaaa
    11821 ccggcatcga ctatgaaatc cgcagcatgg aaaaaccgtc cgatcacgcc cccgtctggg
    11881 cgaccttccg ccgctaattt agctgttctc ctggctcaaa ctgggtcagg agaattaacc
    11941 ttgagaaaaa tcaacaaact gtcagtaatg atttgttgcc tgccgtcctt tgttataccg
    12001 tcactgcgtt tttagttgtc tgaccacttc tctattatca agtttgatat aggaaactcc
    12061 acgatgaacg ctgagcgtaa atttcttttt gcctgtctta tttttgcgct ggtcatttac
    12121 gctttccacg ctttcggttt attcgagctg ctcaccgatt tacctcactt actgacactc
    12181 attcgccaga gcggattgtt cggttatagc ctctatattc tgttattcat cattgccacc
    12241 ctctttctgt taccaggaag catactggtg atcgctggag gaatagtttt tggcccgctc
    12301 ttagggacac tactatcatt aattgccgcc acgctggcct cctcgtgctc attcctgctg
    12361 gcgcgctggc tgggacgtga tttactgctg aaatacgttg gccatagcca tacctttcag
    12421 gccattgaaa aaggcattgc gcgtaacggt attgattttc ttattctgac ccgcttaatc
    12481 ccgttgtttc cttacaatat tcaaaattac gcttacggat taaccacaat cgccttctgg
    12541 ccttataccc ttatttcggc actcacaacc ctgcccggta ttgttattta taccgtgatg
    12601 gcaagcgatc tcgccaatga aggcattacg ctgcgcttta ttttacaact ctgtctggcg
    12661 ggcctggcac tgtttattct cgtccagctc gcaaaactct acgcccgaca caaacatgtg
    12721 gatctgtctg cttcgcgccg cagcccactt tctcacccca aaaatgaagg atagaacgat
    12781 gttgcaacat tattcagtgt catggaaaaa aggactggct gcactctgct tactggctgt
    12841 tgccgggctt agcggctgcg atcaaaaaga gaatgcggct gcaaaagtgg aatacgacgg
    12901 actttcgaac agccaaccgc tgcgtgtcga tgccaataac catacggtaa ccatgctggt
    12961 gcaaattaat ggtcgtttcc tcaccgacga cactcgtcac ggtattgtgt ttaaagatgg
    13021 ctccaacgga cataaatcgc tgtttatggg ttatgcgacc ccgaaagcct tttatgaagc
    13081 cctgaaagag gcaggtggta caccgggcga aaacatgacg atggataata aagaaacgac
    13141 tcatgtcaca ggcagcaaac tggatatttc ggtcaactgg caaggggcgg caaaagcgta
    13201 ttccttcgat gaagtgattg ttgacagtaa tggaaagaaa ctggacatgc gctttggcgg
    13261 taatttaacg gcagcagaag agaagaaaac gggctgcctg gtgtgtctgg atagctgccc
    13321 ggtcggcatc gtcagcaatg caacatacac ttatggtgcg gttgaaaaac gaggtgaagt
    13381 taaattcaaa ggcaatgcct cggttctccc ggcggataac acgctggcaa cggttacctt
    13441 taaaatcacc gaataaagca ggataaagga tgatgaagat gcaatcgcga aaaatctggt
    13501 actaccgtat aaccctcatc atcctgttgt tcgctatgct gctggcatgg gcgctgcttc
    13561 ccggcgtcca tgagtttatc aatcgcagcg ttgcggcgtt tgccgccgtg gaccaacagg
    13621 gtatagaacg ctttattcag tcttacggcg cactggcggc agttgtctcg ttcttgttga
    13681 tgattttaca ggccattgct gcaccgctac ctgcgttttt gatcaccttt gccaatgcgt
    13741 cgctgtttgg cgcgttctgg ggcggcttgc tgtcgtggac cagttcgatg gccggcgcgg
    13801 cgctgtgctt ttttatcgcc agagtgatgg gccgcgaagt ggtggaaaag ttaacgggta
    13861 aaacggtact caatagtatg gacggctttt ttacccgcta tggtaaacac accattctgg
    13921 tatgccgatt attgcctttt gtgccttttg atcccgtaag ctatgctgcc ggtttgactt
    13981 caatacgttt tcgctcgttt tttatcgcca ccgggcttgg tcagttacca gcgactattg
    14041 tttattcctg ggcgggcagc atgttaacag gcggtacttt ctggtttgtc accggactgt
    14101 ttattctgtt tgccctgacc gtggtgattt ttatggcgaa gaaaatatgg cttgagcgcc
    14161 agaagaggaa tgcctgatgg gtttaccgcc gcttagcaaa attcctttca ttttacgtcc
    14221 gcaggcgtgg ctgcatcgtc gccattacgg cgaggtgcta agcccgattc gctggtgggg
    14281 gcggatcccg tttatctttt atctggtgtc gatatttgtc ggctggctgg agcgcaaacg
    14341 ctcaccgctg gatccggtag tgcgatcgct tgtcagcgcg cgcattgcgc aaatgtgcct
    14401 gtgtgagttt tgcgtggata tcaccagtat gaaagtcgcc gagcgcactg gcagcaccga
    14461 taaactgctg gcagtggctg actggcggca aagcccgctc tttagcgatg aagaacggct
    14521 ggcgctggag tacgcagaag ccgcaagcgt aacgccgcca acggtcgatg atgccctgcg
    14581 cgcccgactg gttacgcatt ttgacgctca ggcgctcacc gaactgacgg cattgatcgg
    14641 actgcaaaac ctgtcagccc gttttaattc tgccatggac attcccgctc aggggctgtg
    14701 ccgtattcct gaaaaacgtt cttaaggaga gatgatgcgc cattgtgtgt ggttgctggg
    14761 attgttatcg ctgttttctt tggcaacaca tgccagtgac tggcaggaaa tcaaaaacga
    14821 cgccaaaggg caaaccgtct ggtttaacgc ctggggcggc gataccgcaa ttaaccgcta
    14881 tctcgactgg gttagcggcg agatgaaaac ccattacgat ataaacctga agattgttca
    14941 tctcgcggat gccgcagacg cggtgaagcg cattcagacc gaagctgctg ccggacgcaa
    15001 aacaggcggt tcggtggatc tgctctgggt gaacggcgaa aacttccgca ccttaaaaga
    15061 ggctaattta ctgcaaaccg actgggcaga gactctgccc aactggcgct atgtcgacac
    15121 acagcttccg gtgcgggaag atttttcagt gcctacagaa ggggctgaat cgccctgggg
    15181 cggcgcacaa ctgacgttta tcgcccgccg cgatgttacg ccacagccac cacaaacgcc
    15241 gcaagcctta ctggagtttg ctaaagccaa tcccggcacg gttacctatc cgcgcccacc
    15301 ggactttacc ggcacggcgt ttcttgaaca gttgctgatt atgttgacac ccgatcccgc
    15361 cgcattaaaa gaagcgccgg acgatgcgac tttcgcccgt gtcactgcgc ccttgtggca
    15421 atttcttgat gcgctgcatc cgtatttatg gcgcgaagga aaggatttcc cgccttcgcc
    15481 cgcgcggatg gatgctctgc tgaaagctaa cacattgcgc ctgtcgctga cctttaatcc
    15541 tgctcatgcg cagcaaaaaa tcgccagcgg cgatttaccc gccagcagtt acagctttgg
    15601 ctttagcgag gggatgattg gcaacgtcca tttcgtcacc attcccgcca acgcgaatgc
    15661 cagtgctgcg gcgaaggtag ttgccaattt cctgctctca cccgatgcac aactgcgtaa
    15721 agcagatccc gctgtctggg gcgatccttc tgttctcgat ccgcaaaaac tgcctgacgg
    15781 gcagcgcgaa tcattgcaat cgagaatgcc gcaggatctg ccgccggtac tggctgaacc
    15841 gcacgcaggt tgggtaaatg cgctggaaca agaatggcta caccgttacg gtacgcatta
    15901 atctttttgc tgtgggcgat gatggcggtg atttatgcac cgctgatccc ggcagttctc
    15961 acgctgatat cgcctgcttt gtcgttgacg cactggcagg cgttatttgc cgatccacag
    16021 ttaccgcagg cattactggc aacgctggtg tcgacaacca ttgcggcggt cggggcgttg
    16081 ttgattgccc tgctggtgat tgtagcgctg tggccagggc cgaaatggca gcgtatgtgc
    16141 gcccgtctgc cgtggctgct cgccattccc catgtggctt ttgccaccag cgcccttctg
    16201 ctctttgctg acggagggct gctttatgac tatttcccgt atttcactcc gccaatggac
    16261 aaattgggta ttgggctggg cctcaccctg gcagtgaaag aaagcgcatt tctgctgtgg
    16321 atcttagcgg cagtattgag cgaaaaacgg ctgttgcagc aactcattgt gctggattcg
    16381 ctgggctaca gccgctggca atgcctgaac tggctgctgt tgccctctgt cgcgcctgcg
    16441 ctggcaatgg cgatgctggc gattgtggcc tggtcgctgt cggtcgtgga tgtggcgatt
    16501 atccttgggc caggtaatcc cccgacgctg gcagtaatta gctggcagtg gttaactcag
    16561 ggcgacgccg atcaacaagc gaaaggcgca ctcgccagcc tgctgctgat gctgttactc
    16621 gccgcctacg ttttgctggg ctatctgcta tggcgtagct ggaggcgtac tattccccgc
    16681 gtagatggcg ttcgtaagcc tgccacgcct ttattgcctg gcattactct ggcgagtttt
    16741 ttacccttga ccggtgtgct gtgtgtggtt ctgttggcga tcctcgcgga tcagtcgacg
    16801 atcaatagtg aagcgctcat caatagcctg acgatgggac ttaccgcgac gtttatcgct
    16861 ttaatcctga tactggcgtg gctggaatgg ggatcgtcac gtcggcattt ctggctatgg
    16921 ttgcccattt tattacccgc tctgccactg gtggcgggcc agtacacgct ggcgctatgg
    16981 ctaaacctag atggaagctg gacggcggtg gtctgggggt atctgctgtg ggtaatgccg
    17041 tggatgctgt ttatcctgca acccgcctgg cagcgcattg attcacggtt aattttgatt
    17101 gcgcaaacac tgggctggtc gcggtccaaa atcttctttt acgtgaaatg tccgcttatg
    17161 ctacgcccta cgctgattgc cttcgcggtg ggattttcag tcagtattgc acagtatatg
    17221 ccgacgctgt ggctgggcgc ggggcgtttt ccgacgctca ccactgaagc ggtggcatta
    17281 agcagcggcg gcagcaacgg cattctcgcc gcccaggctt tatggcaact gctattaccg
    17341 cttattattt ttgccctgac cgcgttagtc gcaaaatggg taggttatgt cagacaagga
    17401 ctccgctaat gctctgcgtg aaaaatgttt cgctacgttt accagaaagc cgcttgctga
    17461 caaacgttaa ctttacggtg gataaaggtg acattgtcac gttaatgggg ccgtctggct
    17521 gtggaaaatc cactctgttt tcatggatga ttggtggact ggccggacag ttttcttgta
    17581 caggggagct atggctcaat gagcaacgga ttgacatgct acccaccgca cagcgtcaga
    17641 ttggcattct tttccaggat gcactgttat ttgaccagtt cagcgtcggg caaaatttac
    17701 tgctggcgct accggcgaca cttaaaggga atgcccgacg taatgccgtg aatgatgcac
    17761 ttgagcgttc aggccttgac ggagctttcc atcaggatcc tgctactttg tctggcggtc
    17821 agcgagcgcg cgttgctctg ctacgcgccc ttctcgccca gccaaaagcg ttactcctgg
    17881 atgagccatt cagccgtcta gatgtggctc tgcgcgataa ttttcgccag tgggtgttca
    17941 gcgaagttcg cgcccaggcg atccccgtcg ttcaggtaac gcacgatctc caggatgttc
    18001 ctgctgatag ttctgttctg gatatggcgc agtggtcaga aaattacaac aaactgcgat
    18061 aacgcaaagt ttttctcaat gcgtcagttc agaatggcgc actcaaaact acaatgtcgg
    18121 gattttcgat gaaacgtgtt tctcaaatga ccgcgctggc aatggcttta gggctggctt
    18181 gcgcttcttc gtgggccgct gaactggcga agcctcttac acttgaccag cttcaacaac
    18241 aaaatggcaa agcgatagat actcgcccca gcgcgtttta taacggctgg ccacaaacct
    18301 taaatggccc ttctggtcat gaacctgccg ccttaaacct ctctgctaac tggctcgaca
    18361 aaatgagcac cgaacagctc aacgagtgga tcaagcaaca taacctgaaa gccgatgctc
    18421 cggtggcgct gtacggtaat gacaaagatg tcgacgccgt caaaaagcga ctgcaaaaag
    18481 caggttttac gcatatctcc attctgagtg acgcgctaag cgaaccttcc cgtctgcaaa
    18541 aactgccgca ttttgagcag ctggtttatc cgcaatggct gcacgacctg caacaaggta
    18601 aagaggttac ggcgaaacct gccggtgact ggaaagtcat tgaagcggcc tggggcgctc
    18661 ctaagcttta ccttatcagc catattcccg gcgctgacta catcgatacc aacgaagtgg
    18721 aaagcgaacc gctgtggaac aaagtttctg atgaacaact aaaagcgatg ctggcaaaac
    18781 acggcattcg ccatgacacc acggtcattc tgtacgggcg tgacgtatac gctgcagcgc
    18841 gtgtggcaca aattatgctc tacgctggcg tgaaagatgt ccgcctgctg gacggcggct
    18901 ggcaaacctg gtccgacgcg ggactgcccg ttgagcgcgg aacgccaccg aaagtgaaag
    18961 cggaaccgga tttcggcgtg aagatcccgg cacaaccgca actgatgctt gatattgaac
    19021 aagcgcgtgg actgctgcat cgccaggatg catcgctggt gagcattcgt tcgtggccag
    19081 aatttatcgg tacgaccagc ggttacagct atattaaacc aaaaggtgaa atagccggag
    19141 cacgttgggg acacgctggt agcgactcga cgcatatgga agatttccat aacccggatg
    19201 gcaccatgcg tagcgccgat gatattaccg ctatgtggaa agcatggaat atcaagccag
    19261 atcagcaagt ttcattctac tgcggcaccg gctggcgcgc gtcagaaacc tttatgtacg
    19321 cacgcgcaat gggctggaat aacgtctccg tttatgacgg tggctggtac gaatggagca
    19381 gcgatccaaa aaatccggta gcaaccggtg aacgcggccc ggacagcagt aaataacatt
    19441 gcaattactg acgctggagc gacttcagcg tcagataccc actccacacc cgcgtaaagg
    19501 tcgtcatcca gcacaacgca ccaaataccc acgcaaacca cggaaaacag gccggaaaga
    19561 ggcaactcag aacaaacagc aaaatcgtct cgctgccttc ggttaagccg cccagataat
    19621 aaaacgattt atgcgcataa ccgggattat caatctgatg tttcgccgcc agcgcagcaa
    19681 aagcgagaaa actgctgccc gtgccgataa acgcaaacaa cagccagcct ccggccagcg
    19741 cgttttgttc tggtgcggcg agaataaaac caaacggcac cagcgcgtaa aagagaaaat
    19801 cgagagaaat atcgagaaag ccgcccgcgt cggtaagtcc tctgcgccgc gccagcgcac
    19861 cgtccagacc atcaagcagc ctgttcaaca aaatgacgat tagcgccgcc agataccagc
    19921 ccagcgccag aaacggcagt gccagcacgc cgatggcaaa tcccactaat gttaaaccat
    19981 ctggcgtgat gccgggttta tccagcacct tcacacactg atgcaacaaa ggtttaattc
    20041 ggggatgaag atggcggtct agcacaggca ctccttaaat ataaagcctt tccgattgag
    20101 caacagtgcg gatattatgg catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg
    20161 attgaagttg ttgccgccat cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc
    20221 gcccagagcg atcaggcggg attatgggag tttgccggtg gtaaagtcga gccggatgaa
    20281 agccagcggc aggcgctggt gcgtgagtta aacgaagaac tgggcatcga agcaactgtt
    20341 ggtgactatg ttgccagcca tcagcgagaa gtttcggggc ggattatcca tcttcatgcc
    20401 tggcacgtac ccgacttcca cgggacgtta caggcacatg aacatcaggc gctggtctgg
    20461 tgctcacctg aagaggcgct gcaatatccg ctggcccctg ctgacattcc attattagag
    20521 gcgtttatgg ctttacgcgc cgccagacca gcggattagt gctaaggttt ttgtcatcct
    20581 gttggcattg cagcagtatt ccttcggctt taattaccgc cccttcagaa taattttgat
    20641 cctgataaac gcagcactga gtacagggct gcgctgactg cccgcctgaa ctgaacactt
    20701 ctggcggtac gtttacctcc acgtccggac gataatgcgg gttagccagt gcaattattg
    20761 gaaatgctaa tactacggcg aacaatgctc gactcacagg gaactcctta acgttattgt
    20821 ctctgctact gataacggta gccgggtggc aaaactttag cgtctgagtt atcgcatttg
    20881 gttatgagat tactctcgtt attaatttgc tttcctgggt catttttttc ttgcttaccg
    20941 tcacattctt gatggtatag tcgaaaactg caaaagcaca tgacataaac aacataagca
    21001 taatcgtatt aatatataag ggttttatat ctatggatca gacatattct ctggagtcat
    21061 tcctcaacca tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg
    21121 aagtaatgac cacactctgg ccttttcttg aacaaaatcc caaatatcgt cagatgtcat
    21181 tactggagcg tctggttgaa ccggagcgcg tgatccaatt tcgcgtagta tgggttgatg
    21241 atcgcaacca ggtgcaggtc aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc
    21301 cgtacaaagg cggtatgcgc ttccatccgt cagttaacct ctccattctc aaattcctcg
    21361 gcttcgaaca aaccttcaaa aatgccctga ctactctacc gatgggcggt ggtaaaggcg
    21421 gcagcgattt cgacccgaaa gggaaaagcg aaggcgaagt tatgcgtttt tgccaggcgc
    21481 tgatgactga actgtatcgc cacctgggcg cggatactga cgttccggca ggtgatatcg
    21541 gcgttggtgg tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata
    21601 ccgcctgcgt cttcaccggt aagggccttt catttggcgg cagtcttatt cgcccggaag
    21661 ctaccggcta cggtctggtt tatttcaccg aagcaatgct aaaacgccac ggtatgggtt
    21721 ttgaagggat gcgagtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa
    21781 aagcgatgga atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg
    21841 atgaaagcgg attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg
    21901 atggtcgagt ggctgattac gccaaagaat ttggtctggt ctatctcgaa ggccaacaac
    21961 cgtggtctgt accgattgat atcgccctgc cttgcgccac ccagaacgaa ctggatgttg
    22021 acgccgcgca tcagcttatc gccaatggcg ttaaagccgt cgccgaaggg gcaaatatgc
    22081 cgaccaccat cgaagcgact gaactgttcc agcaggcagg cgtactgttt gcaccgggta
    22141 aagcggctaa tgctggcggt gtagcaacat cgggcctgga aatggcgcaa aacgctgcgc
    22201 gcctgggctg gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc
    22261 accatgcctg tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggggcga
    22321 acattgccgg ttttgtgaag gttgcggatg cgatgttaag ccagggcgtg atttaagaca
    22381 attgtgccgg atacagcgca aacgccttat ccggccataa aaatcttcag tatcagatgg
    22441 ttgaaagaac ctttatatta tcactccatt gcccgcctct tctgatggcg ggcttttttt
    22501 cggcgctctc ttgcgtggcg aatccttttt cttcccttct ccaccgccgg gagccattat
    22561 tccccgaaac tgccttaccg acgtgcgctt tgcctgatca atcaactggt acaatgtccc
    22621 caccagcggc tgcataaagt cctgatagcg acactgcttt tcgctgattt gcgtcagcac
    22681 cgattcccag tgcgcggtca tgtccggtcg cgtcgccatt tctggcagcg aatggaatag
    22741 cgcttttccg gcgtcggtgg agtggatata gcgccctttt ttggtcagga agcctcgctt
    22801 gaacagcagt tcaataatcc cggcgcgagt cgcttctgtc cccagaccat cggtcgcacg
    22861 aaggatcttt ttcagatctt tgtcctgcac aaaacgtgct atcccggtca tcgctgaaag
    22921 cagtgttgca tcggtaaaat ggcgcggcgg ctgggtttgc cgctctacca cttcaccttt
    22981 ttcacacagc aactcatcgc ctttcgccac cacaggcagc ggcgtgccgt cgttttcttc
    23041 atcgcgctct ttgctgccta acagtgtgcg ccagcctgct tcagcaagaa aacgggcttt
    23101 agcgacaaat ttgcctttgg caatgtccag ttcgataaca cacttgcgga acaccgcatc
    23161 cgggcagaac tgcatcaaat actgacgggc aatcaggtta tagaccttcg cttcgttctc
    23221 cgtcaggttg atcgcagaac tccgtgcggt cgggatgatg gcgtggtgcg catcgacttt
    23281 tttgtcatcc caacagcggt tgcgtatatc tggatcgacc actggctgcg gcaacagatc
    23341 tggtgcatga acgctgatag cattcatcac cgcgtggcgt ccggcaaaat gttcttctgg
    23401 caaatagcgg caatcggaac gcggataagt gattagcttg tgcgtttcgt acagcttctg
    23461 gcagatatca agcacgttct gcgcgctcag accaaaacgt ttagccgctt caatctgcaa
    23521 cgccgaaagc gaaaaaggca gcggcgcgga ttctgattcc cgtttatcgt tatagctggt
    23581 gacaatagct ggttgaccac taatgcggtt aaccacatgc tccgccagtg gacgatgtaa
    23641 taagcgccct tcttcatcct ggtacggttc acacgcttcg ctcggttgcc agatagcggt
    23701 aaaccgctca tcggcaggtg tcacgatatg tgctttgact tcaaagaagt ctttcgccac
    23761 gaagttttca atctcttcgt cgcggcgcac caccagtccg agtacgggcg tctgcacgcg
    23821 tcccacggaa agcacgccct gataaccggc attgcgaccg agaatggtat acgcacgggt
    23881 catattaatg ccgtacagcc agtcagctcg cgcacgcgcc agtgcagaaa cgcacagcgg
    23941 tacaaactcg ctattggagc gaagacgatc gatcgcccgc tcaaccgcct gcgggttcag
    24001 gtcgtttatc aaacaacgct gtacctgctg gcgcttttcc ggtgccagtt gcaggtagtc
    24061 cagcacttca tccaccagca gttgcccttc gcgatccggg tctccggcgt gaacgatttc
    24121 gctggcttca tgcaggaagc gtttgatgac attaagttgt ttagtcacgg agggtcgggg
    24181 ctgtaattgc cacttttccg ggacaatcgg caaatccgca agattccagc gcgcatagcg
    24241 gctgtcgtag gcgtctggct gcgcctgctc aagcaggtga ccgatacacc aggtcaccac
    24301 ctgaccatta ccgcactcga taaagccatc gcctttccgg tgcggtttag gcaggacatc
    24361 agcaatggcg cgcgccagac tcggtttttc ggcaataaac aaccgcattg aattaacgga
    24421 tctcaaccat ggcacgaccg ccgcgcgctg gcaccagttc gccaatcgcc gtcagttcaa
    24481 tgccaaactc ggcggctgta gctttgacct cattttctgc ttccggcatg accgccagca
    24541 gcaaaccgcc agaagtttgc ggatcgcaca gcagttcgcg cacttcacgc ggcatttcac
    24601 ccatcagatg accgtagctg gcaaagttac gttcggtgcc gccaggcact gcgcccaact
    24661 taatgtactc ttcaaccccc ggcaatttcg ggatcgcgtc atagtcgacg cgcgcctgca
    24721 cacctgcgcc ctgacacatt tcgcttaaat gccccagtaa accaaaaccc gtaacgtcgg
    24781 tcatcgcttt tacgccttcg atgttggcaa aggacgcgcc tgcgatgttc atccggcaca
    24841 tcacttccgt cgccagtccc tgatgttctg gtttcagcag tgattttttc tcagccgtgg
    24901 taagaacgcc aatccccaga ggtttcgtca ggaacagttt gcatccggct tgtgcggtac
    24961 tgtttttctt cacccgctcg gtaggtacga tccccgttac cgccagacca aaaatcggct
    25021 ctggcgcatc gatggagtga ccgccagcca gcgcaatccc cgcctgacgg catgcatggc
    25081 gtccaccttc ggtcacttcg cgggcaattt ccggggaaag tttgttaatc ggccagccga
    25141 ggatcgcaat cgccataatc ggtttgccgc ccattgcgaa gatatcgctg atggcgttag
    25201 tcgccgcaat gcggccaaaa tcgaaaggat tatcaacgat cggcataaag aagtcggtgg
    25261 tactgataac gctggtgcca ttgcccagat cgtataccgc cgcatcgtcg cgggtttcat
    25321 tacccacaag caaattcgga tcaacaaact tcgcctgctc actgtgcagg atggtttcca
    25381 acacttttgg ggaaatttta cagccgcaac cagcgccgtg gctgtattgg gtcaaacgaa
    25441 tcgagttctc gctcatggac atctcctgtc aatgcaatcc gggtatggta accctcattc
    25501 catgaagtga taagtgagag tgtctgaatt cctgcgcctt tgctcacaat ccagacagtt
    25561 tcgcgacaat tatcagaaat aagtcacaaa cggcgtcggg tccgggacgt ttatcgacgt
    25621 agatgctttc aactgcggcg taccgaggta gagaaaaccg acaattttat cctgctcacg
    25681 gcaaccgaat gcttcacgca ctaccggact ttcagttaat gcaccactgc gccagatgcc
    25741 gccaaacccc tgggcaactg ctgccatttg catcgccatg accgcgcatc ccgcagacat
    25801 ttcctgttcc cagcgcggga ctttatgatt ttcttcgcat ttcgccacca ccgttatgat
    25861 gagcggtgcg cggaacggcg cattacgcgc tttgtcgatg gctttgttat cactaccggc
    25921 agcaatcgcc ccctgctcca gtacggcgct gaaacgctcg cgcccttccc cttcaatcac
    25981 aaagaaatgc cacggttgca tggacttatg gtccggcgca cgcatagccg cacgcaggat
    26041 gttttgcagt tgttcacccg ttggcgcagg ttcagccagg cgggaggcgc tacggcgatt
    26101 gatcaatagt tcgagtgcat ccatttgatt aactcctgtc atgatattta ttcacaaaat
    26161 taacacgaga gtggattttg ttacagccca gtccgcaatt cctgctgaca agtgccggtt
    26221 gggtcattac gataaccaca tctattgcgc ctgaaacagg tgtgacctta agttgggaga
    26281 atacatgcga accctttggc gatttattgc cggatttttt aaatggacgt ggcgtctgct
    26341 gaatttcgtc cgtgaaatgg tacttaacct gttctttatt tttctcgtgc tggttggtgt
    26401 ggggatctgg atgcaggtca gtggtggtga ttcgaaagaa acggccagtc gtggcgcact
    26461 gctgctggac atttctggtg tgatcgtcga taaacccgac agttctcagc ggtttagtaa
    26521 attaagccgc cagctgcttg gtgccagttc cgatcgtctg caggaaaact cactgtttga
    26581 tatcgtcaac accattcgcc aggcgaagga cgaccgcaat atcaccggta ttgtgatgga
    26641 tctgaaaaac ttcgcaggcg gcgaccagcc gtctatgcag tacatcggca aagctctgaa
    26701 agagtttcgt gatagcggga aaccggttta tgccgttggc gagaactaca gccaggggca
    26761 atattatctc gccagtttcg ccaataaaat ttggctgtct ccgcaaggcg tggttgatct
    26821 gcacggcttt gccaccaacg gtctgtacta caaatcatta ctggataagc tgaaagtttc
    26881 cacccatgtg ttccgcgtgg gtacgtataa atctgccgtt gaaccgttta ttcgtgatga
    26941 tatgtcgccg gcagcccgcg aagctgacag ccgctggatt ggtgagctgt ggcaaaacta
    27001 tctgaatact gttgccgcta accggcagat ccctgctcag caggtattcc ctggcgcgca
    27061 agggttgctt gagggtttaa ccaaaaccgg tggcgatacc gcgaaatatg cgctggaaaa
    27121 caagctggtc gatgcactgg catcgagtgc cgaaatcgaa aaaactctga ccaaagagtt
    27181 cggctggagc aagactgata aaaattatcg cgccatcagt tattacgatt acgcattgaa
    27241 aacgccggca gataccggtg acagcatcgg agtcgtcttc gctaatggcg caattatgga
    27301 tggcgaggaa actcagggga atgttggcgg tgataccact gcggcacaaa tccgcgacgc
    27361 tcgccttgac ccgaaagtga aagcgattgt cctgcgtgtt aatagcccag gcggcagcgt
    27421 taccgcgtct gaagtgattc gcgctgaact ggcagcagcc cgggcagcgg gtaagcctgt
    27481 ggttgtatcg atgggcggca tggcggcatc tggtggttac tggatttcca caccagctaa
    27541 ttacattgtg gctaacccca gcaccctgac cggttctatc ggtatcttcg gcgtgatcac
    27601 caccgtagaa aatagtctgg attcgattgg tgttcatact gatggtgtct caacttcacc
    27661 gctggcggat gtttctatca ccagggcact gccgccggaa gcgcagcaga tgatgcagtt
    27721 aagcattgag aatggctata aacgctttat cacgctggtt gctgatgcgc gtcattcaac
    27781 gccagagcag attgataaaa tcgcccaggg ccatgtctgg accggtcagg atgcaaaagc
    27841 taacgggctg gtcgatagtc tcggggattt cgatgatgcg gtcgccaaag cagcagagct
    27901 ggcaaaagtg aaacagtggc atctggaata ctacgttgat gaaccgacct tcttcgacaa
    27961 agtgatggac aacatgtctg gttctgtccg ggcaatgttg ccagatgcgt tccaggccat
    28021 gttacctgca ccgctggcct cggtagcctc taccgttaaa agtgaaagcg acaagctggc
    28081 agcgtttaac gacccacaaa accgttatgc gttttgcctg acctgcgcca acgtgcgtta
    28141 agtcttgtac tgagtggccg acagatcgtc ggccacattt ttttacctcg acgaatcctc
    28201 ttcccgctgt ctcgccccat atttccttat ccgcatagta tcaggtgcgc tcccccttcc
    28261 tcacgtatat acttttgctc tttcgatatc attcatatca atatcatgca aaagaaatca
    28321 atttacgtcg cctacacggg cgggaccatc gggatgcagc gttccgagca gggttatatt
    28381 ccggtgtcag gtcatctaca gcgccaactg gcgctgatgc cggaattcca tcgcccggag
    28441 atgccagatt tcactattca tgaatatacg ccattgatgg attcttctga tatgacgcca
    28501 gaagactggc agcatattgc tgaagatatt aaagcgcact atgacgacta tgatggtttt
    28561 gtcattctgc acggcacaga cacgatggcg tataccgcct ctgcactgtc gttcatgctc
    28621 gagaatctcg gtaaaccggt cattgtgaca gggtcacaaa tcccgctggc tgagttacgc
    28681 tctgacggac aaattaatct gctgaatgcg ttgtacgttg cggcgaatta tcctatcaac
    28741 gaagtaactc tcttttttaa taatcgactg tatcgtggca acagaaccac caaagcccat
    28801 gccgatggtt ttgatgcgtt tgcctctcca aaccttcctc cgttactgga agcaggtatc
    28861 catattcgcc gtttgaatac gccacccgcc ccgcacggtg aaggggaatt gatcgttcat
    28921 ccaatcacgc cgcaaccaat tggcgtagtg acgatttatc cggggatttc tgctgacgtc
    28981 gtgcgcaatt ttctgcgcca acctgtgaaa gcattgattc tgcgctcata tggcgtgggt
    29041 aatgcgccac aaaacaaagc cttcctgcag gaattacaag aagccagcga tcgcggtatt
    29101 gtggtggtca acctgacaca atgtatgtcc ggtaaagtga acatgggtgg ttatgccacc
    29161 ggtaacgccc tcgcccatgc cggcgttatt ggcggtgcag atatgactgt agaagccacg
    29221 ctaaccaaac tgcattacct gcttagccag gaacttgata ctgaaaccat tcgcaaggcc
    29281 atgagccaaa acctgcgtgg cgaactgacg ccggatgatt aaggagactg caatgcaccc
    29341 tcgcgccctg ttactggtcg atttacaaaa tgatttctgt gctggtggcg cgctcgccgt
    29401 gccggaaggt gacagtacgg tggatgtcgc taaccgcctg attgactggt gccagtcgcg
    29461 cggtgaagcg gttatcgcca gtcaggactg gcacccggcg aatcacggca gttttgccag
    29521 tcagcacggt gtagagcctt atacgccagg ccaactcgac ggtttgccac aaaccttctg
    29581 gccagatcac tgtgtgcaga acagtgaagg cgcacaatta catccgttac tgaaccaaaa
    29641 agcgatcgca gcggtgttcc ataaaggcga aaatccttta gttgacagtt acagtgcctt
    29701 ttttgataac ggccgtcggc agaaaaccac gctcgatgac tggttacgcg atcatgaaat
    29761 cgatgaattg atcattatgg gtctggctac tgactattgc gtgaagttta ccgtgctgga
    29821 cgcgttacag ttaggttata aggttaatgt gattaccgat ggttgtcgtg gcgtgaatat
    29881 ccagccacag gacagtgcgc acgcgtttat ggagatgtca gcagctgggg caacgctata
    29941 tacgctggca gattgggaag agacgcagga gtaatattag ttggattcat ctctggccta
    30001 catttcctca atgacattgt aggccaggta atacttgtta gtgttgcatt taacctactc
    30061 gcagctaaaa cacgacatcc gaacaagtct atttgccctc ccttaataac aggcttaaat
    30121 actagacaac tcattcactt aaacatggca ataggctttg ctgagatacc aaaatcttct
    30181 tttaactgtt gtttgctctt catgaccatc tgaccatttg tgtcaattgt catatgctgt
    30241 gcttcggtat tattgcgggc ttgccacagc atcaccagtt gcaggcagtt ctctttttgt
    30301 tcctcggtca acgtaacgcc atcaggccat ttccccagtt caacggcggt cgacaaacgc
    30361 tggtatacct caggcgtcat gctgttgata atgtcatcaa gattcataat ttttccgctc
    30421 ctgtggaata agttgctgaa tcgttttttc aaccgttgat ttcttcgccg ttttcgccat
    30481 cagtgaagcg taaagaagca gagttaacac aataacgttc gcccgttggc tgcggcccgt
    30541 cagggaagac atggcccaga tgggcatcgc agttaccgca acgaatttct atgcgctgca
    30601 ttccatgtga caagtctttg atataacgaa tagattcttc actcaccggt tcgtagaaac
    30661 tgggccagcc acagccggaa tcatacttgg tttgggaatg aaacagcggg gcatcgcaga
    30721 tcaaacagtg atatacgccg tcacgcttgt tatgcagtaa acgacccgta aatggcggtt
    30781 ctgtcccatg attctgcgtc acgtagaact gcatctcgga caaatttttt ttcagttctt
    30841 ctgccgaagg tttattagcc atttgctcac atctcacttt aatcgtgctc acattacgtg
    30901 actgattcta acaaaacatt aacaccaact ggcaaaattt tgtcctaaac ttgatctcga
    30961 cgaaatggct gcacctaaat cgtgatgaaa atcacatttt tatcgtaatt gccctttaaa
    31021 attcggggcg ccgaccccat gtggtctcaa gcccaaagga agagtgaggc gagtcagtcg
    31081 cgtaatgctt aggcacagga ttgatttgtc gcaatgattg acacgattcc gcttgacgct
    31141 gcgtaaggtt tttgtaattt tacaggcaac cttttattca ctaacaaata gctggtggaa
    31201 tatatgacta tcaaagtagg tatcaacggt tttggccgta tcggtcgcat tgttttccgt
    31261 gctgctcaga aacgttctga catcgagatc gttgcaatca acgacctgtt agacgctgat
    31321 tacatggcat acatgctgaa atatgactcc actcacggcc gtttcgacgg taccgttgaa
    31381 gtgaaagacg gtcatctgat cgttaacggt aaaaaaatcc gtgttaccgc tgaacgtgat
    31441 ccggctaacc tgaaatggga cgaagttggt gttgacgttg tcgctgaagc aactggtctg
    31501 ttcctgactg acgaaactgc tcgtaaacac atcaccgctg gcgcgaagaa agtggttatg
    31561 actggtccgt ctaaagacaa cactccgatg ttcgttaaag gcgctaactt cgacaaatat
    31621 gctggccagg acatcgtttc caacgcttcc tgcaccacca actgcctggc tccgctggct
    31681 aaagttatca acgataactt cggcatcatc gaaggtctga tgaccaccgt tcacgctact
    31741 accgctactc agaaaaccgt tgatggcccg tctcacaaag actggcgcgg cggccgcggt
    31801 gcttcccaga acatcatccc gtcctctacc ggtgctgcta aagctgtagg taaagtactg
    31861 ccagaactga atggcaaact gactggtatg gcgttccgcg ttccgacccc gaacgtatcc
    31921 gtagttgacc tgaccgttcg tctggaaaaa gctgcaactt acgagcagat caaagctgcc
    31981 gttaaagctg ctgctgaagg cgaaatgaaa ggcgttctgg gctacaccga agatgacgta
    32041 gtatctaccg atttcaacgg cgaagtttgc acttccgtgt tcgatgctaa agctggtatc
    32101 gctctgaacg acaacttcgt gaaactggta tcctggtacg acaacgaaac cggttactcc
    32161 aacaaagttc tggacctgat cgctcacatc tccaaataag ttgagatgac actgtgatct
    32221 aaaaagagcg acttcggtcg ctcttttttt tacctgataa aatgaagtta aaggactgcg
    32281 tcatgattaa gaaaattttt gcccttccgg tcatcgaaca aatctcccct gtcctctccc
    32341 gtcgtaaact ggatgaactg gacctcattg tggtcgatca tccccaggta aaagcctctt
    32401 ttgcattaca gggcgcacac cttctctcat ggaaacctgc gggtgaagaa gaagttctgt
    32461 ggttgagcaa taacacaccg ttcaaaaatg gcgtcgctat tcgcggtggc gtaccggttt
    32521 gctggccgtg gtttggtccg gctgcacaac aaggtccggc tgcacaacaa ggtctgcctg
    32581 cgcacggttt tgcccgcaac ctgccgtgga cgctgaaatc acatcgtgaa gatgctgatg
    32641 gcgtagcgct gacttttgaa ttgacacaaa gcgaagagac gaaaaaattc tggccgcacg
    32701 actttacgct attagcgcat ttccgcgtgg gtaaagcctg tgaaatcgat cttgaagcac
    32761 acggcgaatt tgaaaccacc tctgccctgc atacctactt taacgtgggt gatatcacta
    32821 aggtaagcgt cagtggactg ggcgatcgct tcattgataa agtgaatgac gcgaaagaag
    32881 atgtactgac cgacggtatt cagaccttcc ctgaccgtac cgatcgcgtg tatctgaatc
    32941 cgcaagattg cagcgtgatt aatgacgaag cgctgaatcg tattatcgcc gtaggccacc
    33001 agcatcatct gaacgtcgtt ggctggaacc caggaccggc gctttcagtt agcatgggcg
    33061 atatgccgga cgatggctac aaaacctttg tttgtgtaga aacggcttac gcttcagaaa
    33121 cgcaaaaagt gaccaaagaa aaacctgcac atctggcgca atccattcgc gttgcgaaac
    33181 gttaatttgc gttaatgttg tgtgccgggt gcgttgcatc cggcacgtat caacatcaca
    33241 ccatatccag cgcagttttt ccttttggtg ccggatatgc cttatccagc atagctaatt
    33301 ccgctgacga aagttcaacc tcaagcacag ccgcattttg ttggacatgg gcaatcgtgg
    33361 ccgcttttgg aatcgccatc acaccctgat gactgatcac ccacgccaac aatacttgtg
    33421 ccgcgctgat attgtgagca tgtgcaattt cgttgactac tgcgtttttt agcagcccat
    33481 tgcgcaaccg cccggcctga gctaacggac tgtaagccat caccggtatc tgctgttgct
    33541 ggcaccaggg gagtagatcg tactcaatcc ctcgtgaacc gagatggtaa agcacctgat
    33601 tagtggcaca ctgatttccc cccggcagct gccagagttc ctgcatgtcg gcataatcaa
    33661 ggttagaaac gccccagcgg cggatttttc cctgggcgat caatttttcc atcgctgcga
    33721 cagtctcttc aaaagcgaaa ctgccagacc agtgtaataa gtagagatcg agataatcag
    33781 tattgagacg gcgtaaactg gcttcgcatg catttatcgc tttttgcccg ccagcattcc
    33841 acggatagac tttagagacg agaaagacgt tatcacgcag accggttaat gcttccccaa
    33901 ccaccttttc agcaccgcct tcggcataca tttcggcggt atcaatgagg gttaaaccga
    33961 gttcaatgcc cgcgcgtagt gcagcaactt ctgttttgcg ctgactggca tcttcgccca
    34021 tataccatgt tccctgccct atggctggca gtgagacatc gccactaaat tgaatcattt
    34081 tttgttgcat tgtttcctcc caggtattgc accaccgtaa tgcaaaacag ggcatgacgc
    34141 cctgttttta tgcacaaaat gccctggaaa gatgcattat cagaatttgt aggtgatccc
    34201 ggtagaaatc aggccagtcc aggatttatc caccattggg ctgtcagtaa cttcatcaga
    34261 cagacgggtg tagcgcgcgg taccgtaaac actccagtcg ccgaggaagt tgtagctggc
    34321 gctcagctcc aggtaagggc tccagctgtc gttcgggtta tagccacgca gaccgctgcg
    34381 agcggactct ttgcgcgata cgccataata gtattcgttc tggttttcgc tgttccactg
    34441 cacaccaata cccggagtca cggtcaggcc accgttggtg taacgataca accaggccat
    34501 atcccagacg atgccgttgc tgttatccag ggtatcgcca gccagggtgg tacgcaggta
    34561 accgtactgg gtaaagtgag cataagacag accagccatc atggtgctct tacggtcatc
    34621 caggtgacgc atttggtgat cgccactgtc tttagctttg aagtaaagcg gcgaccagta
    34681 agcggtaatt gaaagtttgt ccgttgcgtc attccacagg tagtaaccac cacctaagcc
    34741 acggaaccag aagttatcgc cgtcatagtt gattaccggt actgggtaaa catcggtatc
    34801 gtaatcttta tatgggtgtt caacgacacc tacgcctgcg cccagggaaa atttaccttc
    34861 agcgtgcgct acgcctgcag acgttgcgat aagcactcca agtgccagaa gtttgagttt
    34921 ggtcacaatt aatcattcct taaacaaatg tttagcgggc gacaaagttt acccgtcaat
    34981 acatcgaaac ccaacctttt tacgttttca ttttttatag taactgttta attttcctga
    35041 cgcagatgac accactctta cagccaaatg aatttagcgt taccggcgac cctggtattt
    35101 acattaatta tgcaaaattt atggatgagt tgttgatatg ccattgaaat taagaaagcc
    35161 gtgcaggcaa gttttccatt tgccatctac gcttaatttt gaaggtgtat caccgggcac
    35221 gttgttctca tcgtcgataa aatggcatga gagttgctgt gttttagcaa gagacgtcgt
    35281 tcagtttact tcttccggga gcctctacta ttcatatgaa cggctcttaa cctgtgctaa
    35341 aaaacgaaag gacggcatac catgaatata ttcgatcact atcgccagcg atatgaagct
    35401 gccaaggacg aagagttcac actgcaggag tttcttacca cttgtcggca agatcgcagt
    35461 gcttatgcca acgcggctga gcggctattg atggctatcg gtgagcctgt catggtcgat
    35521 acagcccagg aacccagact ttctcgactc ttttctaacc gggtcatagc acgttatccg
    35581 gcatttgaag agttttacgg catggaagac gcgattgaac agattgtctc ttatctgaaa
    35641 cacgcggctc aggggctgga agagaagaaa caaatcctgt atctgcttgg gcctgtgggt
    35701 gggggtaaat catcgcttgc tgagcgactg aaatcattaa tgcagctcgt accgatttat
    35761 gtattgagcg ccaacggtga gcgtagcccg gtcaacgatc atccgttctg tcttttcaat
    35821 ccgcaggaag atgcgcagat tctggaaaaa gagtatggca ttcctcgccg ttatctcggc
    35881 accatcatgt cgccgtgggc ggcaaaacgc ctgcatgaat ttggtggcga tatcactaag
    35941 ttccgggtag tgaaggtctg gccgtcaatt ctgcaacaaa ttgctatcgc caaaacggaa
    36001 cccggtgatg agaacaacca ggacatctcc gcgctggttg ggaaagtcga tattcgtaaa
    36061 ctcgaacact acgcgcagaa tgacccggac gcctacggct attccggtgc actgtgccgc
    36121 gccaaccagg ggatcatgga attcgttgag atgtttaaag caccgattaa agtgctgcat
    36181 cccttgctaa ccgccactca ggaaggtaac tacaacggga cggaaggtat ctccgccctg
    36241 ccgttcaacg ggattattct cgcccactcg aacgaatccg aatgggtcac tttccgtaat
    36301 aacaaaaaca acgaagcctt cctcgatcgt gtttacatcg tgaaggtgcc gtattgcttg
    36361 cgcatttccg aagagatcaa aatctacgag aaattgctta atcacagtga attgactcac
    36421 gccccctgcg cccctggcac gctggaaacg ctgtcacgtt tttccattct ttcgcgcctt
    36481 aaagagccag aaaactccag catttattca aagatgcggg tttatgatgg cgaaagtctg
    36541 aaagacacag atcccaaagc caagtcgtat caggaatatc gtgactacgc cggtgtcgat
    36601 gaagggatga acggtctgtc gacgcgtttt gcgtttaaga tcctctcccg cgtgttcaac
    36661 ttcgatcatg tagaagtggc ggcaaacccg gttcatctgt tctacgtcct ggaacagcag
    36721 atcgagcgcg agcagttccc gcaagaacag gcagaacgct atctggagtt cctgaaaggt
    36781 tatctgatcc cgaaatatgc cgagtttatc ggcaaagaga tccagacggc ctaccttgaa
    36841 tcctattccg aatatgggca aaacattttc gaccgttatg ttacctacgc ggatttctgg
    36901 attcaggatc aggagtatcg cgatcctgat accgggcagt tgtttgaccg cgagtctctt
    36961 aacgccgagc tggagaaaat cgagaaaccg gcggggatca gtaatccaaa agatttccgc
    37021 aacgagattg ttaacttcgt actgcgcgcc agagcgaata acagcggacg caatccgaac
    37081 tggaccagct atgaaaaact gcgcacggtt attgagaaga aaatgttctc caataccgag
    37141 gagctgttgc cggttatttc gtttaacgcc aaaacgtcaa ccgacgagca aaagaaacat
    37201 gacgactttg tcgaccgtat gatggaaaaa ggctacaccc gtaaacaggt gcgtttactg
    37261 tgcgaatggt atttgcgcgt acgtaaatcg tcttaacaac cctggcccgg tacacatgct
    37321 accgggccta caacgacagc gaaccgtggg ccagagaagc ggcaacacag gcgtagcata
    37381 aagttggcaa atgtagtacg gggggcatat gacctggttt attgaccggc gtctgaacgg
    37441 caaaaacaaa agcatggtga atcgccagcg ttttttacgc cgttataaag cgcaaattaa
    37501 acagtcgatc tccgaggcca ttaataagcg ttcggtgact gacgtcgaca gcggcgagtc
    37561 cgtatccatt cccacggaag atattagcga accgatgttt catcaggggc gtggcggtct
    37621 gcgccaccgc gtgcatccgg gcaatgacca tttcgtccag aacgaccgaa ttgaacgtcc
    37681 ccagggtggc ggcggaggtt ccggcagcgg tcagggccag gccagccagg atggtgaagg
    37741 tcaggatgaa ttcgtctttc agatttcgaa agatgagtat cttgatctgc tctttgaaga
    37801 tttggcttta ccgaatctga aacaaaacca acaacgccag ctgaccgaat ataaaacgca
    37861 tcgggcgggt tataccgcta acggcgttcc ggccaatatc agcgttgtgc gttcattgca
    37921 gaactcactg gcgcggcgca cagccatgac ggcgggcaag cggcgggaac ttcatgcgct
    37981 ggaagagaat ttggccatca tcagcaacag tgaacctgcg caactgctgg aagaggaacg
    38041 tctgcgcaaa gaaattgcag aattacgtgc caaaattgaa cgcgtccctt tcattgacac
    38101 cttcgattta cgttacaaga actacgaaaa acggcccgat ccctccagcc aggcagtgat
    38161 gttttgcctg atggacgttt ccggttcaat ggatcagtcc actaaagata tggctaaacg
    38221 tttttatatt ctgctgtatc tgttcctcag cagaacatat aagaacgtgg aagtcgtata
    38281 catccgccat catacccagg cgaaagaagt cgatgaacat gagtttttct actcgcagga
    38341 aacaggcggc accattgttt ccagcgccct gaaactgatg gatgaggtag tgaaagagcg
    38401 ttataacccg gcacagtgga atatttacgc agcccaagca tcagacggcg ataactgggc
    38461 cgatgactct ccgctttgcc atgaaattct ggcgaaaaaa atattacccg ttgttcgtta
    38521 ttacagctat atcgaaatta cacgtcgcgc gcatcagaca ctgtggcgag aatatgagca
    38581 tctgcaatct actttcgaca actttgcgat gcagcatatc cgcgaccagg atgatattta
    38641 tccggtgttc cgtgaactgt ttcataaaca aaatgcaaca gctaaagact aaaactgtca
    38701 gccgggtcat tatcgcctgg ctgattttta gttcactgta aattatctca tttattacat
    38761 acattagctt acaatcgctt taaatatgac agcataacct ttacacaatt tagttccaga
    38821 aaacaatcat tcggaaaaat gattcggtca acacgtattt ccctggggtt attctttaat
    38881 tattttttat cgttaacgaa aattgatctt ggtcaaaact atatatctct gccatcaata
    38941 aaatccagca ctcacattgc tctccttttt atggcttcta tgggtacaca aaaattaaaa
    39001 gctcaaggct tttttatttt cagcttattg ctgacgttaa ttttattttg cattactacc
    39061 ttatataacg aaaacacgaa tgtaaaactc atcccacaga tgaattacct gatggttgtt
    39121 gtggctttgt ttttccttaa cgccgtcatt ttccttttca tgttaatgaa gtatttcact
    39181 aacaaacaaa ttttaccaac actcatttta agccttgcat ttttaagtgg ccttatctat
    39241 ttagttgaaa ccattgtaat tatccataaa ccaatcaacg gcagtacact gatccagaca
    39301 aagtcgaatg atgtttctat tttctatatt ttccgccaac tcagtttcat ttgtttaacc
    39361 tcgctggcgc tcttttgtta tgggaaagac aacatccttg acaacaataa gaagaaaact
    39421 ggcattctgt tgctggcgct gattcctttt ttagtttttc cccttctggc acacaatctg
    39481 agcagttata acgctgacta ttctttgtat gtcgtcgatt actgtccgga caaccatact
    39541 gcgacctggg gaatcaacta tacaaaaata ttggtttgtt tgtgggcatt tttactgttc
    39601 tttattatca tgcgtacacg attagccagc gaactatggc cgttaatagc attattatgt
    39661 ctggcatcgc tatgctgcaa cttacttcta ctgactctgg atgagtataa ttacaccatc
    39721 tggtatatca gtcgcgggat tgaagtttcc agtaaactgt ttgttgtgtc ttttctgatt
    39781 tataacattt ttcaggaact gcaactctcc agcaaactgg cagttcatga tgtactgacc
    39841 aatatttata atcggcgcta ctttttcaac agcgtagagt cattattgtc gcgacctgtt
    39901 gttaaggact tttgtgtcat gctggttgat attaatcagt tcaaacgcat caatgcccaa
    39961 tggggacatc gtgtgggtga taaagtgctg gtatcaattg tcgatattat ccagcaaagc
    40021 atccgccccg atgatatttt agcgcgactg gagggtgagg tgtttggctt gctatttacc
    40081 gaactcaata gtgcccaggc aaaaatcatt gcggaacgta tgcgtaaaaa tgtcgaactc
    40141 ctgaccggct ttagtaacag atatgatgtt cctgagcaaa tgaccatcag tattggcacg
    40201 gttttttcaa cgggtgacac acgtaatatc tcgcttgtca tgacggaagc agatcaagcc
    40261 ttacgcgaag cgaaaagcga ggggggcaac aaagtgatta ttcatcatat ttaagtgcaa
    40321 aaatattcag agccatgctt tttgcgtggc ttttgcatac aatttattac tattacccct
    40381 aaatttcccc tccccagcgg cgcagagatg agtataatta gcgcccctgt gccaggccgc
    40441 aatcgaactt tatctggttt tctcgtttca ctaaccgaag gagtgccatt tatcatgaaa
    40501 ttgcaccata gaatgctccg gcattttatc gccgcaagtg tcattgtgct gacatcttcc
    40561 ttccttattt ttgaacttgt cgccagcgac agagcaatga gtgcctatct gcgctatatc
    40621 gtgcagagag cagactcctc ctttctttat gataagtatc agaatcagag tattgccgcg
    40681 catgtgatgc gcgctctcgc tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc
    40741 atctgcgagg cttttgagtc tgccaataat acccatggct taaacctgac tgcccataaa
    40801 tacccggact tacgcggcac actacaaacc gcatccactg actgcgacac aattgtggaa
    40861 gctgcagcac tattacccgc ttttgatcag gcagtggaaa gcaaccgcca ccaggatgat
    40921 tacggttcag gtcttgggat ggccgaagag aaatttcact attatctcga tctcaatgac
    40981 cgctatgtct atttttatga accggttaat gttgagtact ttgcgatgaa taactggtct
    41041 ttcctgcagt caggaagtat tggcatcgat cgcaaagata ttgaaaaggt atttaccggg
    41101 cgtaccgttt tatcgagcat ttaccaggat cagcgtacta aacagaacgt gatgagtttg
    41161 ctgacgccgg tatatgtcgc agggcagcta aaagggattg tgctgctgga tattaacaaa
    41221 aacaatctgc ggaatatctt ttacactcat gaccgccctc tcctctggcg ttttctcaat
    41281 gtcacgctaa ccgataccga ttcggggcgc gacattatca tcaaccagag cgaagataat
    41341 ctgttccagt atgtcagtta cgtccatgac ttaccgggcg gcattcgtgt ctcgttatcc
    41401 attgatattc tttactttat cacgtcttcg tggaaaagcg ttctgttctg gattttgacg
    41461 gcgttaattt tgctgaatat ggtgcggatg cacttccgtt tataccaaaa cgtgtcgcga
    41521 gaaaatatta gtgatgcgat gactggactg tataatcgca aaattttaac ccctgaactg
    41581 gagcagcggt tgcagaagct ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac
    41641 atggacaagt taaagcaaat aaatgacacc ctcggtcatc aggaggggga tttagcgatt
    41701 acgttattag ctcaggcgat taaacaatcg attcgtaaaa gtgattatgc catccgactc
    41761 ggtggcgatg aattctgcat cattcttgtc gattcgacgc cgcaaattgc agcacaactg
    41821 cctgaacgta tcgaaaaacg tctgcaacat atcgcgccgc agaaagagat cggcttctct
    41881 tccggtattt acgcgatgaa agaaaacgat acgttacatg atgcgtataa agcttccgat
    41941 gagcgtttat atgtcaataa gcagaacaaa aacagccgtt catgataacc ttctgtggtt
    42001 gtttgcttgt aatctcagga gcatgaaatg actgaaatgg ctaaaggaag cgtgacgcat
    42061 cagcggttaa tcgctttatt atcacaagaa ggtgctgact tccgcgtcgt tacccatgaa
    42121 gcggtaggta aatgtgaagc ggtgtcagaa attcgtggca ccgctttagg ccagggggct
    42181 aaggcactgg tatgtaaagt caaaggcaat ggtgtaaatc aacatgttct ggcgatcctc
    42241 gcggccgatc aacaggccga tctgagccaa ctcgccagcc atataggcgg attacgcgct
    42301 tcgttggcca gcccggcgga agtcgatgaa ctcacaggct gtgtcttcgg cgcgatcccc
    42361 cctttcagct tccatccaaa actcaaactg gttgccgatc ccctactctt cgaacgattt
    42421 gatgaaatcg ccttcaatgc aggcatgctg gataaatccg ttattttgaa aaccgcggat
    42481 tatttgcgca ttgcgcaacc agaactggtc aatttccgcc gcactgcgta actagcctgt
    42541 ccgttcgata agaagaacgg acaaaaccag cacaacagca atggcaaaaa acgatgacgt
    42601 gataatcagt gtctcgacga acatttgatc gttcatagta tgcccccggg agatagttgt
    42661 tttcacgtta ttgttagcgt gcgcaaatgg cagtttgatg acagttcgcc tgatttttta
    42721 tgcaaaaaac gtaaagattt tctacttcct tcctgcagca agcgtaaagt aagcaggctt
    42781 attattcttt ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc tgctcgggtt
    42841 agctgcgctg ggctttatca gtcataacac cactgtcgcc gtttcaattc tggtgttaat
    42901 cattgtccgc gtcacaccgt taagcacctt tttcccgtgg attgaaaaac aggggctaag
    42961 tatcgggata atcattctga cgattggcgt tatggctccc atcgccagcg ggacgctacc
    43021 gccctcaacc ctgattcact cgtttctgaa ctggaaatcg ctggtggcaa ttgcagtcgg
    43081 cgttattgtc tcctggctgg gggggcgcgg cgtgacgtta atgggcagcc agccgcaact
    43141 ggtcgccggg ctgctggtgg gcactgtttt gggcgtggcg ctgtttcgcg gcgtaccggt
    43201 tggcccgctt attgccgcgg gtctggtttc gctgattgtg gggaaacagt agttaatccc
    43261 gcaatatatc ggcctggcgt ttgccccagg cctttcttaa acatagtaat aaaggccgtt
    43321 gttgaatcat accccagcgt atgagcaact ttctgtacag tatcgccttt cactaatccc
    43381 tgaagcgcca taatcagctg taactgctga cgccactggc gaaaactcac ccccgtctct
    43441 ttcactatca gtcgggcaag attgcgttca ctcatcgcaa aaaagcgagc ccattgcccc
    43501 aatgaccccc actcgacggg ctctttcgcc atcatctcca ccatcgcgcg gattttagga
    43561 tgagaagaaa cgggtaggtg cagctgttgt tgtggctgct gagggagttc atcaaacagg
    43621 acctgaatta atctgcgggt catcggctca gcacgctgag tcgtcgtcct gtttgccagg
    43681 gttaaaatta attcccggca taagggcgat attttcaatg tgcaacaggt tgtcggcatt
    43741 gtcactgcgc tgggttcgat aaacagaaaa cagagttcag cgttagctgt cacttgattg
    43801 ctatgttcca cccctcccgg tatccaaacc gcatattgtg gcggcaccat ccacaaagca
    43861 ttttccaccg tacaggtaat tgcaccatgt agtgccagta tcaactgtcc tttgcggtgc
    43921 tgatgtacag gactggttaa ctcatccgtt ccggcgtgaa tacaaaatgc gaccgcggct
    43981 tcatggtgtc ggtcgggttc atagccattg agattcaacc tgtgcatcat tttgtccgaa
    44041 tttagcgata atttgtcatt ttagcttgat tcaacataac aataaaaacg gtaaggtaca
    44101 gcctcgtttg taacaatgag aagcatatga cctgttcaac ttcattaagc ggcaaaaaca
    44161 ggattgtcct tatcgctggc attctgatga ttgccacaac attacgcgtc acctttaccg
    44221 gcgcagcacc gttactggat acgattcgtt ccgcttactc gctgacgacg gcgcaaaccg
    44281 gcttattgac caccctgcca ttattagcct ttgcgctaat ctcacctttg gctgccccgg
    44341 tggcgcgacg ttttggtatg gaacgtagcc tgtttgccgc gttacttttg atctgtgctg
    44401 gtatcgcaat tcgctctctc ccttcgcctt acttattatt tggcggtaca gcggtcattg
    44461 gcggtgggat tgcattaggc aatgtcttac tgccaggatt aattaaacgc gatttccctc
    44521 attccgtcgc cagacttacc ggcgcatatt ccctgacaat gggagctgca gcggcactgg
    44581 gatcgactat ggtcgtgccg ctggctttga acggttttgg ctggcaaggc gcgttgctca
    44641 tgctgatgtc ttttcctctg ctggctcttt ttttatggct gccacagtgg cgaagtcaac
    44701 aacatgcaaa tttgagtacc tcacgcgcct tacatactcg gggtatctgg cgttcgccgc
    44761 ttgcctggca ggtaacattg tttcttggga tcaactcact ggtctattac gtgattattg
    44821 gctggcttcc ggcgattctc atcagtcacg gctatagcga agcacaggcg ggttcactgc
    44881 atggtttgct gcaactagcc acagcagctc ccggtttgct gatcccactt ttcttacatc
    44941 atgtgaaaga tcagcgtggt attgcagcgt tcgttgcctt gatgtgcgca gtggccgcgg
    45001 ttgggctctg ctttatgcca gcacacgcga tcacctggac tctgcttttc ggttttggtt
    45061 ccggcgcaac aatgatactg gggttgacgt tcattggtct gcgggcaagt tctgcgcatc
    45121 aggcggcggc actctcgggg atggcacaat ccgtcgggta tttgctggca gcctgtgggc
    45181 cgccgctgat gggtaaaata cacgatgcta acggtgactg gtccgtacca ctgatgggtg
    45241 ttgcaatact ttcactactg atggcgattt tcggactttg cgccgggaga gacaaagaaa
    45301 ttcgctaata tccggtgcta tagtgacgtg gcaaatcatg cgtgaaaggg agaacaaata
    45361 cgatgaatat tcagtgcaaa cgcgtttatg atccggctga acagagcgat ggttatcgcg
    45421 tactggtcga ccgcctctgg ccacgcggta tcaaaaaaac cgatttagcc cttgatgaat
    45481 gggataaaga aatcacgccg tcaacggaac tgcgcaaagc ctttcacggc gaagtcgtcg
    45541 attttgcaac ctttcgcgag caatatcttg cagaactggc gcaacacgag caagaaggaa
    45601 agcggctggc gaacatcgcc aaaaaacagc cgctgaccct gctctactca gcaaaaaaca
    45661 ccacgcagaa ccatgcgctg gtgctggcag actggctacg tagcttgtga ttttagtacg
    45721 ccatccggca gttatttttc accagccgga tggtcccgtc gccacaatgc ccattcatca
    45781 atcgtttcac cgcccggtaa tttgcaattg ttgctgaccc cttgcgctgt ctgcacagga
    45841 atgagcgtcc cgcccttctg ctggcaatag accgacgccg gatttgccat accaatctgc
    45901 ggcggtttag gtgcttctgg ctgagaaggg gttgaacaac cagccaggac cagcaagcaa
    45961 ggcagaacaa aactgataat tttcatttat tgatctcaca tatttatcca gacttagagt
    46021 atcgcggtat cgttttgttt tgcagcacta tttttattac attcactcaa atcatattac
    46081 gccttgtttc atctttgttg atgatgtttt atcatgcctg caaagattaa ataatcagca
    46141 tttacccgcc gtatcctgga gttgtttcgt gtcagatcag attatcgccc gcgtctcgca
    46201 atcccttgcc aaagaacagt cactggaaag cctggtccga cagcttctgg agatgctgga
    46261 aatggtcact gatatggaat caacctacct gaccaaagtg gatgtcgaag cgcgcctgca
    46321 gcatataatg tttgcccgta acagccagaa aatgcacatc ccggagaatt ttaccgtctc
    46381 gtgggattac tcgttatgca aacgcgccat tgatgaaaac tgctttttca gcgatgaagt
    46441 ccctgaccgt tggggcgact gtattgcggc acgcaatctt ggcatcacca catttctgag
    46501 cacgccaatt cacttaccgg atggatcatt ctatggcacg ctttgcgccg ccagcagtga
    46561 gaagcgccag tggagtgaac gcgcggaaca ggttttacag ttattcgccg gactgattgc
    46621 acaatatatt caaaaagagg cactggttga gcagctgcgc gaagccaatg ctgcgctgat
    46681 tgcgcaatcg tataccgact cgttaaccgg gctaccgaat cggcgggcga tttttgaaaa
    46741 tctgacgacg ctgttttctc tcgcccgaca tcttaaccat aagataatga tcgcgtttat
    46801 cgatctggat aacttcaaat taatcaacga tcgttttggt cataatagtg gtgatttgtt
    46861 cctcattcag gttggcgagc gccttaatac gctccagcaa aatggcgaag ttattggtcg
    46921 tctcggcggt gatgagtttt tggttgtttc actgaacaac gagaatgcgg atatttcgtc
    46981 tctgcgagaa cgtattcaac agcaaatacg tggagaatat cacttaggtg acgttgattt
    47041 gtattatccc ggtgccagtc ttggcatagt agaagtcgat cctgaaacga ccgatgcaga
    47101 cagtgccctg catgctgccg atatcgcgat gtatcaggag aaaaaacaca aacagaaaac
    47161 accttttgtc gcgcatccag cgctacattc ctgaggcgta ttcacatcct tttgattggt
    47221 gataacatgc gaatcggtat tatttttccg gttgtaatct ttattacagc ggtcgtattt
    47281 ttagcatggt tttttattgg cggctatgct gccccgggag cataaagatg aaaaaaacaa
    47341 cgattattat gatgggtgtg gcgattattg tcgtactcgg cactgagctg ggatggtggt
    47401 aacgtcacat ctagaaaata gcaaaggctg cctgtgtgca gcctttgtgc aatttacgcg
    47461 ttaactttta atctttctgt agataaacag cacgacaatc gcgccaataa cggcaaccac
    47521 gaagctgcca aaattgaagc catcgacttt accaaagcca aacagcgtgc tgatccatcc
    47581 gccgactacg gcaccgacta tccccagcag gatagtcata aagaatccac caccatcttt
    47641 acctggcatg atccacttcg ccagaatacc ggcaataagc ccaaaaataa tccatgacag
    47701 aatgcccatt gtttcctcac ttatctgttt tgcattagcg ggttagtcgc tgataaaaag
    47761 catagcacaa catcgtgagg gcaagatttg tgacgagcat cacggaggat ttttttgcga
    47821 tggcgcagaa attgcgccat caacgatcag tgataattac caaccacaaa catcatgttc
    47881 gttttccgtg tcataagaac gcacggtatt caccagatct tttatcactt cagccgccac
    47941 ttctggcacc agcaaagtca tcggcgtctc tgtttcataa tcgacagaaa cgccattgct
    48001 gttattggtg acggtcacgg tatacgttgc tttgcccatg attcatttcc cgttatgaat
    48061 gactttccgt tgttgcgcac cttccatcag gacttcagga gcaacgaaga agtcaatgtt
    48121 gaaataagtg tcgtcagtca tggcttcaat gttgtgccac ttttctggag ggaacacagc
    48181 aaactgcccc gcttcgataa ggatcacttg atcaggctct gtactgtgtt catcagcgta
    48241 gccgagatat ttgaccgccc catgcataac ggaaaggcgt gggtaaaccc ccgggcgcgt
    48301 tcctttatca agatgacgtt cgaatattcc ggcaggcgca gtttgtttat tccagaaagg
    48361 cgttgagcgc gtatgaatat aattctgtgg gatttgcagc atccttttcc ctccttcggt
    48421 gaatgcgctg aaaacggttt atttcagccg tttcagggta cgcctgataa tttgcatttt
    48481 aaataccatt tattggttac tatttagcac cgtatcagag aagaatcagg gaggattaca
    48541 ggtgggaagc ccgcgacaac gcgggctgaa tgcatcagga ctggagcgtc gccagtcggg
    48601 cggcgaaacc cacaaacatc aaaccaatca gtgagttgcc cactttagcc agtttctttt
    48661 tggtacgtat gtactgcgtg acaaaagcac cagaaataat caggaagctc aaatagcaga
    48721 aactcaccag ttccagcgtc gtcgccagaa taaagaatga aattcccgta tgtggggcat
    48781 taacatcgat aaactgtacg aaaaacgaca catagaacaa aatggctttc ggattagtca
    48841 ggctcaaaat taacgcgcgt ttaaaaatgg caccgtattg gggctcatcg gatttggcct
    48901 cgttattttt acccttcagg gtcgcgtaga gaattttact ccccagatag agcaaataaa
    48961 acgcaccaag ataacgtaca atgttgaata atatcggggt ggtcttaatt aatgtcgcca
    49021 ctccagccca tgccagaaac atcaataccg catcgccaat aaatacaccg caggccgcaa
    49081 gataaccgcc tttcataccg ctactgacgc tatttttgag tacaaacagg gtatttggcc
    49141 ctggcaccaa cacaataaaa atggccccaa ccagataggt ccagtaattc agaaccccgt
    49201 attcagcgaa cacattaacc tctttaatta tctttcgatc atgcgcgatt aaaggtgaat
    49261 atgctaacca atctgtagcg gcttagaaag gagaaaatca ggttttaacc tgatatcaac
    49321 ccgataattg aatcattaat caggcatgct ccagtgaaaa attcgggtag tgctcggcaa
    49381 aatactggcg taaaaattct accgttatcc gtactttcgc tgacgtcgcc agccttgaaa
    49441 cataaacggc ccagacattc gctggctggt aatattccgg tagcacttgt actaaatgac
    49501 cactggcaat gttttcgcta acatcccacc aggagcgcag agcaatcccc tgcccatcga
    49561 gacaccactg atggacaatt tctccatgat tagacgacag tgggccggta actttaatcg
    49621 catggtggcc ttctttattg cgtaattgcc agacaccaaa aggatgatcg cgctctttaa
    49681 tgaccaggca cggcaatgca gaaagatcgg ttaaatgttt cggcgcgcca tgttgcgcaa
    49741 taaattccgg tgacgcgcag agaatgcgat aattggtcgc cagtttgcgg gcgattaaat
    49801 taggggcgat gtcatcgcca atgcgaatat cgagatcgac accttcattg accaaatcga
    49861 ccaaccggtc ttccacatca aaacgtaatt ccagttgcgg atacgccttc gccagcgctg
    49921 ataacgccgg ggccaccacc tgtcgtccaa aaccaaagct gctgataata cgcagcatcc
    49981 cctgtggcac ctgacgcacg tcagaaagtt cgtccatcat ctgaccgaca tcctgcaaaa
    50041 tccgctgcgc ccattcataa atccgctctc cttcttcggt aatggtgacg cggcgggtgg
    50101 tgcggtgtaa caacaccacg tttagcgttt gctccagcaa ggcgatgcgc ttgctgacga
    50161 acgccggaga aacgcccagt tcttccgcca cggcggcaaa accagcccgg cgagccacca
    50221 gcataaagac gcgcaaatcg ttcagcagcg gtaaattatt catgattcgt gttttatgtt
    50281 tcaccagtta cagggattaa ttccttttca gtcaattata ggatggtgat gttgtcaatt
    50341 ttgatggtca ggaagtgaga acccaatgat gaaaacgatg cgtattgctg cgatcccggg
    50401 agacgggatt ggcaaagaag tccttcctga agggattcgc gtgttacagg ctgccgctga
    50461 gcgctggggc ttcgccttga gttttgagca aatggagtgg gcgagctgcg agtattacag
    50521 ccatcacggt aaaatgatgc cggacgactg gcatgagcaa cttagccgtt tcgacgccat
    50581 ctattttggt gccgtcggct ggccggacac cgttccggac catatttcgt tgtggggttc
    50641 gctgctgaaa tttcgtcgtg aattcgacca gtacgtcaac ctgcgcccgg ttcgtctctt
    50701 tcctggcgtt ccctgcccgc tggcgggaaa acagcctggc gacatcgatt tttacgtggt
    50761 cagggaaaac accgaaggcg aatattcctc gctcggcggc agagtgaatg aaggtacaga
    50821 gcatgaagtc gtcattcagg aatcggtatt tactcgtcgt ggtgtcgatc gtattttgcg
    50881 ctatgccttt gaacttgcgc aaagccgccc acgtaagacg ctaacttctg ccactaaatc
    50941 gaacggttta gccatcagca tgccgtactg ggatgagcga gtggaagcaa tggctgagaa
    51001 ttacccggag atccgctggg acaagcagca tattgacatt ctctgcgcac gttttgtgat
    51061 gcaaccggaa cgcttcgatg tggtagtggc gtccaatttg tttggcgata tcctttccga
    51121 tcttggcccg gcctgcaccg gcaccattgg cattgcccca tccgccaacc tgaatccgga
    51181 acgcactttc ccgtcgctct tcgagcctgt ccacggttcc gcgccggata tctacgggaa
    51241 aaaaattgct aaccctatcg ccacaatttg ggccggtgca atgatgctcg attttctcgg
    51301 caatggagat gagcgtttcc agcaagcgca taacggtatt ctggccgcga ttgaagaagt
    51361 gattgatcac gggccgaaaa cacctgatat gaaaggcaat gccaccacgc cacaggttgc
    51421 cgacgcgatt tgcaaaatta ttttgcgtta aggtcaaacc agtttatttg aaccgcgtca
    51481 ctgacgcggt ttttttattc gttctttgca gtaaataacc tgcgtcattt caccttttat
    51541 tgtttccgtt tcgtgtttta tggctttccg tattcttaat tgtttaattt atgtaacatg
    51601 caaataatca atttgttaat cacgacatac aactcattac cctgcaaata attacaactg
    51661 gcaaaagcca gagacgggct tttatgccca ggagatgact catgatgagc aatgttaaga
    51721 aaaaagatgt gccgctgata agcatcagcc tggtggccat tcttttcatc gcagctgcat
    51781 taagcctttt cccacaacaa tcggccgacg cggccaacgc aatatacact tttgttacgc
    51841 gtacgttagg ttccgccgta caggtattgg ttttgctggc aatgggactg gtgatttatt
    51901 tagccaccag taaatacggc aatattcgtc ttggcgaagg aaaaccggaa tacagcacgc
    51961 tctcatggct gtttatgttt atttgtgccg gtttaggttc ttctacgctt tattgggggg
    52021 ttgctgaatg ggcctattat tatcaaacgc ctggattaaa tatcgcaccg cgttcacaac
    52081 aggcactcga atttagcgtt ccctactcct tcttccactg gggcatcagc gcctgggcaa
    52141 cttatacgct ggcctcatta atcatggctt atcactttca tgtgcggaaa aataaagggc
    52201 tgagcctttc cggcattatt gctgctatta ccggcgttcg cccgcaaggc ccgtggggaa
    52261 aactggtcga tttgatgttc ctgatcgcca ctgtcggcgc actgaccatt tcccttgttg
    52321 tcaccgcagc aacctttacc cgtgggcttt ccgcgctgac cggtttaccc gataacttta
    52381 ccgtacaggc atttgtgatc ctgctttccg gcggcatttt ttgcctaagc tcgtggattg
    52441 gtatcaacaa cggtttgcaa cgtctaagca aaatggttgg ctggggcgcg ttcctgctgc
    52501 cattactggt gctgattgtc ggtccaaccg aatttattac caacagcatc atcaatgcca
    52561 tcggcctgac cacgcaaaac ttcctgcaaa tgagcttatt caccgatccg cttggcgatg
    52621 gttcatttac ccgcaactgg accgttttct actggctgtg gtggatctca tacacgcctg
    52681 gcgtagcaat gtttgtcacc cgcgtttccc gcggtcgtaa gattaaagaa gttatctggg
    52741 gactgatcct cggcagcacc gtcggttgct ggttcttctt cggcgtaatg gaaagctatg
    52801 ccattcacca gtttatcaat ggcgtaatca acgtcccaca ggtgctggaa acactgggcg
    52861 gcgagacagc tgtgcagcaa gttctgatgt cgttgccagc cggtaaattg ttcctcgccg
    52921 catacctggg cgtgatgatt attttccttg cctcgcatat ggatgcggtg gcctacacca
    52981 tggcggcaac cagtacgcgt aatctccagg aaggtgacga tcctgaccgt gggctgcgtc
    53041 ttttctggtg cgtggtaatt accctgatcc cgctttcaat tcttttcact ggcgcgtcgc
    53101 tggaaacgat gaaaaccacc gtcgtactca cagcccttcc gttcctcgtc attttactgg
    53161 tgaaagtcgg cggatttatt cgctggctga aacaggatta cgccgacatt ccggctcatc
    53221 atgttgaaca ttatctcccg cagacaccgg ttgaagtcct ggaaaaaacg ccagtgctcc
    53281 ctgcgggaac cgtattcaaa ggcgacaact gagcgccatc atcctaacga taaaggtatc
    53341 cctatgagca atctgagccc tgactttgta ctacccgaaa atttttgcgc taacccgcaa
    53401 gaggcgtgga ccattcctgc ccgtttttat accgatcaga acgcctttga acacgaaaaa
    53461 gagaacgtct tcgccaaaag ctggatttgc gtcgctcaca gcagcgaact ggcgaatgcc
    53521 aatgattatg tgacgcgtga gatcattggc gaaagcattg tgctggtacg cggtcgtgat
    53581 aaggttttgc gcgcgttcta taacgtgtgt ccgcaccgtg gtcatcagtt gttgagcggc
    53641 gaagggaaag cgaaaaatgt gattacctgc ccgtatcacg catgggcatt caaactcgat
    53701 ggcaacctgg cccatgcacg taactgcgaa aacgtcgcca atttcgatag cgacaaagcg
    53761 caactggttc cggtgcgtct ggaagaatat gccggattcg tcttcatcaa catggaccct
    53821 aacgccacca gcgtagaaga tcagttaccc ggtctgggcg cgaaagtgct ggaagcctgc
    53881 ccggaagtcc acgatctgaa actggcggcc cgctttacca cccgcacgcc tgccaactgg
    53941 aagaacattg tcgataacta tctcgagtgc tatcactgtg gtccggcgca tccaggtttc
    54001 tccgactccg tacaggttga tcgttactgg cacaccatgc acggtaactg gacgctgcaa
    54061 tacggtttcg ccaaaccgtc agaacagtct ttcaaattcg aagagggcac ggatgcggca
    54121 ttccacggtt tctggctgtg gccgtgcacg atgcttaacg tcacaccgat caaagggatg
    54181 atgacggtca tttatgaatt cccggtggat tctgaaacta ccctgcaaaa ctacgatatt
    54241 tacttcacca atgaagagtt aaccgacgag caaaaatcgc tgattgagtg gtatcgcgat
    54301 gtgttccgtc cggaagattt acgtctggtt gaaagcgtac agaaagggct gaaatcgcgt
    54361 ggctatcgtg gtcaggggcg catcatggcc gacagtagcg gtagcggaat ttccgaacat
    54421 ggtatcgccc atttccataa tctgctggcg caggtgttta aggactaatg acatcggcgg
    54481 cggtattttc cgccgctggg ctgatttttg atggagtaca gcaatgtcag actatcaaat
    54541 gtttgaagta caggtgagtc aggtggaacc gcttaccgaa caggtgaaac gcttcacgct
    54601 ggtggcaacc gatggcaaac cgctgcctgc gttcaccgga ggaagtcaca tcatagtgca
    54661 gatgagcgat ggtgataacc agtacagcaa tgcgtattca ctactgagtt caccgcataa
    54721 cacctctagt tatcagattg ccgttcgact ggaggaaaac tcgcgcggcg gttcccgctt
    54781 tttgcatcag caggtaaaag tgggcgatcg gttaacgatt tcaacgccta ataacctgtt
    54841 tgcgctaatt ccctcggcca gaaagcatct gtttatcgcg ggcggtattg gtatcacccc
    54901 tttcctgtcg cacctggcag agttgcaata cagcgacgtc gactggcagc tacattactg
    54961 cacgcgaaat ccagaaagtt gcgcatttcg tgatgagcta gtccagcatc cacaggctga
    55021 gaaagttcac ttacatcact catcaaccgg aacacgactg gaattagcgc gattattggc
    55081 ggatatcgaa cctggcacac atgtttatac ctgtggcccc gaggcgctaa atgaagcggt
    55141 aagaagtgaa gctgcgcgtc tgaacatcgc cgccgatacg ctgcactttg agcaatttgc
    55201 tatcgaagac aaaaccggcg atgcatttac cctggtgctt gcccgttccg gaaaagagtt
    55261 tgtggtgccg gaagagatga ctattttgca ggttattgaa aataataaag cagcgaaagt
    55321 ggaatgttta tgtcgtgaag gggtatgcgg aacctgcgaa acggcaatac tggaaggtga
    55381 agctgaccat cgggatcagt attttagcga tgaagagcgt gccagccagc aaagtatgtt
    55441 gatctgttgt tcgcgcgcga agggtaaacg cctggtgttg gatttgtagt ttgccgaagc
    55501 cggactgagc gcgcccagtc cggcttcgga agattttact gcggatattc ctgcaataaa
    55561 ttgtgtaatg cttccgccat cagctcacca cgccagccgg aaatcagctc cggcaaattg
    55621 ttctgcggtt tcagtttcca gtgccagttc agcagttggt tgatttgccg acgcgatgcc
    55681 agcaattcgg cgctgatctt gtgcgtttcg ctcacgtcag taatcagcga cttaatcgct
    55741 ttaaacgctt tacgataacc cggcatgtcc atcaggttaa gcatcggctg cggtaaggcc
    55801 tcttccggca atgcctgcgc tttttccacc agcgccagca gcgttttacc gtgaaagcgg
    55861 atttcgctac cggataagcc caggctgtcc agttcgccta aacttcccgg catataacgc
    55921 gctacggacc acaaatgctc ttcacgcacg acaaagttca ctgccagatc gcgctcccgc
    55981 gccttgcgca gtcgccagtc ggctaacagt tgcagacagg ccagttggcg tgtgcgtaac
    56041 tgccaggcat tggtgatatc acgccaggca tcttccggag caacgacttc ctgacgacgc
    56101 atttgcatca ggcggcattc atccagcgcc gcaggtagcc agccggaggc ctccgtttct
    56161 accataagct ttgcggtgat cggtaacaga taccagacat ccgccgctgc gtattcacac
    56221 tgacgttcgg tcagcggtct ggccagccag tcggtgcgcg attcactctt gtccagcgta
    56281 acgccggaat actcttccac catggaagcg aaaccccatg acatcgggcg tccgcagaag
    56341 gcagcaagga tttgcgtgtc aatcaagggt tgtggtaatt cgccaaagac attgaggaac
    56401 acttccagat cttcactgcc cgcatggaga aattttgtga tggacggatc gcgcaggatc
    56461 gctttcagcg gtgaccagtc agagatcccg aggggatcaa ttagcgccag atgctcacca
    56521 tcaaaaagtt gaattagacc cagttgcggg taataggtgc gcgtacgaac aaattcagta
    56581 tccagggcta tcgccggaaa ggcacggacg gcttcacaca aagaagccag cgcatcgtcc
    56641 gtggtaatca tttggtaatt caaattgtat tctctttagt gggcgtcaaa aaaaacgccg
    56701 gattaaccgg cgtctgacga ctgacttaac gctcaggctt tattgtccac tttgccgcgc
    56761 gcttcgtcac gtaattctcg tcgcaaaatt tttccgacgt tagatttcgg taactcatca
    56821 cgaaactcca ccagcttcgg tactttgtag cccgtgagct gacggcggca aaaagtcacc
    56881 agtgactctt cggtaagcga tggatctttt ttcacaacga agattttcac cgcttcacca
    56941 ctggagccgg aaggtacgcc aacagccgcg acttcctgta cgccaggatg ctgcatgacg
    57001 acatcttcaa tctcgttggg atagacgtta aaaccggaaa ccagaatcat gtctttttta
    57061 cgatcgacaa tgcgcaggaa tccttcttca tccatcaccg cgatgtcgcc ggtgtgtaac
    57121 cagccatttt tgatgatttc atcggtagca tccggacgct gccagtaacc cagcatcacc
    57181 tgcggtcctt tgacacaaag ctcacccggt tgaccaggtg gtacttcact atcatcatca
    57241 tccaccagtt tggcttccgt cgacggcacc ggcaaaccga tgctaccact atgataatca
    57301 atatcatacg ggttaacgct caccagcggc gcacactcgg tcaggccata accttccagc
    57361 agatactgtc cagtcagttt aacccaacgt tctgccacca cttgctgaac aggcatccct
    57421 ccgcctgcgg aaagatgcag actggagaaa tccagctgct ggaactcttt attgttcagc
    57481 aacgcattga acaaggtgtt aacgccagtg atagcggtaa acggatattt cgctaactct
    57541 tttaccaacc ctggaatatc gcgcgggtta gtgataagca ggttctgccc ccccagttcg
    57601 ataaacagca ggcagttaat ggtcagtgca aaaatgtgat acagcggcag cgccgtcacc
    57661 accagctctt tgcccggatg caacagcgga ccataggtcg cgttaacctg ttccaggttc
    57721 gccagcatat tgcggtgagt cagcatcgcg cctttcgcca caccagtggt gccgccggtg
    57781 tattgcagga aagctaaatc ttccggcacc agttcgggtt tgacgtactg catccggtag
    57841 ccgttgtgca gggcgctacg aaatgaaatg gcatctggca gatggtattt cggcaccaga
    57901 cgcttgatgt atttaacaac gaaattgact accgtgcctt ttgccgtaga tagctgatcg
    57961 cccatacggg tcagaattac gtgctgaacg gcggttttat caaccacttt ttccagagtg
    58021 tgggcaaagt tagacacgat aacaatcgcc gatgcgccgc tatcgttaag ctgatgctca
    58081 agttcacgcg gggtatacaa cgggttaacg tttacgacaa tcatcccggc acgcaaaatg
    58141 ccaaacagcg ccaccggata ttgcaataaa ttaggcatca tcaacgcaac gcgatcgcct
    58201 ttcttcagtc ccaacccttg ttgcaaataa gcggcaaacg cgcgactgcg ttcttccagt
    58261 ttgcggaagg tcatcacctc ccccatattc acaaacgcgg gttgatcggc gtagcgcgcg
    58321 accgactgct caaacatatc taccagagat tgataacggt cagggttgat ctccgtcggg
    58381 acgtccgcgg gataacggtt aagccaaacc ttcttcaatt cttcacctct aaaatgcgtg
    58441 ttcgtcgtca tcgcaacccc aaatgatata catgctgtta acataatatt aactcatcat
    58501 accagcttga taattaccca acgaaaaggt tgcgaagcgc gtcactattt atttttatct
    58561 ttaccgtaag aatgcagaaa cagcggacca gccgctgttt ctttttcttt aaaaacaagc
    58621 aattattcag ttacaacagt ttgtactctc gcggggccgg gattatacca gccccatcca
    58681 ccgtagccat agggccagcc acgaccgcca taaaaccatg gatcaatcgg ctgaggcggc
    58741 ataatcacct gctgggttaa atgccaacgt ttgtaacccg ttacctgcat caccataaat
    58801 ttatagggcg tgttgccgat tttgccgtca accgcaccgg tgattggccc aactaccgta
    58861 accagttgtc cacggaaatc taccgggtcc agaaaaccgt tcacatcagc ataaatgcga
    58921 ccgcgagaag gttctcccag cgtcggtctg gctccgctgt ccagtggtac ggtggcaatt
    58981 tccaggcggg ttttcccttg ctggttttgt accgcaacca ctttgccacc aaagcgtgcc
    59041 tcctgaccaa cgtacagctg cggcgcactc atcacccgaa ctaaatcttg ttgcggcgtg
    59101 ggactgctgc ctttaatggc gtccggcaca gtaacacaac cgctgagcat tagcgcaaac
    59161 gtacctgcca gtatgccttt gataacattc ttttgaaccg ccatgatgcg actccttttt
    59221 tctcagggca tactcttaag attcattctt tgcccggaag tttcttccat gcgacgttgt
    59281 tacgtaaata aaccggttcg gcatgttcca ccgccaccgt tttaccctcg gcaaacatct
    59341 gacacgcaat cggaagcata tcttctgcag cgggcagtaa cacttcgcca tcgcacaggg
    59401 tcaaaccgct ctctttaccg agatccggcc aggcttgcca gcctgtacct accgtcaccc
    59461 attccccgga aagctgttgc atccgttcat gaacgagttc gggtttgagt acggcttcgg
    59521 tttcttcacc gtgccagata cccttttcat cacgctgata ttcggcccag taaacttcgc
    59581 ccatccgcgc atcaatagca gccagcacgc gggttgcgcc gtttttgcgc cacgcccctt
    59641 gcgccattgt catgagcgtg gaaacgccaa tcatcggcag atccgcgcca agcgccagcc
    59701 cctgcgcaat accaatacca atgcgcacgc cggtaaagct accggggccg cgcccgtaag
    59761 ccagagcgtt aatatcagtc agggaagttc cgctggtggt caggatatcc tgcaccatcg
    59821 gtaagattcg ttgagtatgt tcacgagggc aaagctcaaa atgagcgttg acagtaccgt
    59881 cgttccacag ggcgacagag caggcctctg tcgcggtatc gatagccaga attcgcatgg
    59941 gtcttcgtgc ttagatcaat aaaaaggcgc gcatcatacc atactccgta acaaattacc
    60001 tggaggatgg tatcgcaagg aaacgaaccg cacgggcaat gtcacgggtg cgcggcgctg
    60061 gcggcagact ggcgagaaac gtcgcgccgt aagggcgcat caccagccga ttatcgcaaa
    60121 tcaccagcac gccgcgatcg tcggcgtcgc gaatcagtcg ccctactccc tgcttgagag
    60181 taatcaccgc atccggcagt tgtacttcat cgaacgggtc accaccacgc aaacggcaat
    60241 cttccatgcg cgcttttaat agtggatcat ccggtgaggt aaacggcaac ttgtcgataa
    60301 tcaccaatga caatgtatcg ccacgcacat ccaccccttc ccagaagctg ctggttgcca
    60361 caagaagcgc gttaccggcg ctgacaaatt gctgcaacag ttgccctttg ctggtttccc
    60421 cctgcaacaa aacgggaagc gtcatggtag cgcggaactg ctcagccaga tcgcgcatca
    60481 tggcgtgcga ggtacaaagc ataaaacaac gaccgttgtt agcttcgatg atcggtcgca
    60541 acattgccgc cagttgccgt gcggagcccg gttgattggt ttgcggcagg ttgcgcggca
    60601 cacagagtaa cgcctggcgg ctgtaatcaa acgggctggg taacagcaac gactctgcct
    60661 gttcaatgcc aagccgcgag gtaaaatgat gcagatcgtc gttcaccgac agcgttgctg
    60721 aggtaaagat ccagctaccg ggtttttgcg ccattaactc tttgaattta tccgccacgc
    60781 tgagcggcgt gagcgccaga gtaaaatggc gcgaagtgca ttcgtaccag tagctgtagc
    60841 ccggctgatt gatctctttt agccgtttca gccgcgtacg atacaacgtg gcgcgctcaa
    60901 atgccgcatc cagcaaggcg gaacgcccca gcgacagttt cgccacgtca taacaaagtt
    60961 ccagggtgtc atcgagcagt aaaaacgccc gttgaatttg cggattagct aacagttcgc
    61021 gcaggttgcc acgataacca ggctcaccga gttgcagacg aaaatcctgc gcgctctggg
    61081 caaggcggtc ggcgcacttt tgtaactgct gagtatcttt taattcggtg cgataggcga
    61141 tggtgatgtc ttttgccagg tcgagtagtt gtcgactgga gagtgactga ccaaaatact
    61201 ggctggcaat gtcaggcagc tggtgggctt cgtcgaagat catgacgtca gcttccggga
    61261 tcagttcgcc aaatccactc tctttcacca ccatatccgc cagaaagaga tgatggttta
    61321 ccaccaccac atcggcgtcc atcgcttttt tgcgtgcttt gaccacaaag caatctttat
    61381 acatcgggca gtcgctgcca aggcagttat cgttggtgct ggtgaccaac ggccacgcct
    61441 gtgaatcttc cgccacgctg acgcaggtgc tgatatcacc atcgactgtt tgattagacc
    61501 aggagcgcag caggatcaca tcgcttaaga tttgtaccgg cagatcgccc cccgccagcg
    61561 cctgctgttc gagacgttcg aggcagaggt agtttgagcg ccctttcagc aacgccacgt
    61621 tgcccgtata tttcaacgcc tttgaaaccg ttggcaaatc gcggctgtag agctgatcct
    61681 gcaacgcttt tgagccggtc gagataatga ctttcttttt cgcccgcagc gcaggggcca
    61741 ggtaagcgta ggttttgccc gtaccggttc cggcttccac caccagcggc tggccttttt
    61801 ctatcgcctg ggtgacggct accgccatct gtcgctgtgg ttctcgcggc ttaaagcctg
    61861 gtatcgcttt cgccagctga ccgtctggtg caaaatcgtc cgtcacacta ccccctgttg
    61921 atttgaacag ggattatgtc aggatgaggg cgctttcgcc agttgaagtg gtgacggcga
    61981 cctcacattg tggcagtctt tgcagcacga aaatggaagt ataatgagga caaaatgatg
    62041 actatcgttc gtatcgatgc tgaagcccgc tggtctgatg tagtaatcca caacaacacg
    62101 ctctactaca ctggtgtacc ggaaaacctc gacgccgatg cctttgagca aaccgccaac
    62161 acgctggcgc agattgacgc cgtgctggaa aaacagggca gcaataaatc gagcattctg
    62221 gatgccacca ttttcctggc cgataaaaac gacttcgcgg cgatgaataa agcgtgggat
    62281 gcctgggttg tcgcgggtca tgcgccggtg cgctgcacgg tacaagcggg tttgatgaac
    62341 ccgaagtata aagttgaaat taagattgtg gctgcggtgt aagctttatc gaagcaaaat
    62401 aagtcagacg ataatttatc gacaatactg gtcggttttg cataaatcga ccagagatta
    62461 agatattaca gttgatgctc ctgctaattc attatttatt tttataatt
//
LOCUS       DAZGHF010000012        61096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGHF010000012 DAZGHF010000000
VERSION     DAZGHF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.012"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(27..848)
                     /gene="dapB"
                     /locus_tag="ACHK3A_000836"
     CDS             complement(27..848)
                     /gene="dapB"
                     /locus_tag="ACHK3A_000836"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO8738964.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(1015..1929)
                     /gene="rihC"
                     /locus_tag="ACHK3A_000837"
     CDS             complement(1015..1929)
                     /gene="rihC"
                     /locus_tag="ACHK3A_000837"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO8738965.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(1995..2945)
                     /gene="ispH"
                     /locus_tag="ACHK3A_000838"
                     /gene_synonym="lytB"
     CDS             complement(1995..2945)
                     /gene="ispH"
                     /locus_tag="ACHK3A_000838"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO8738966.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKGVNCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(2947..3396)
                     /gene="fkpB"
                     /locus_tag="ACHK3A_000839"
     CDS             complement(2947..3396)
                     /gene="fkpB"
                     /locus_tag="ACHK3A_000839"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO8738967.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(3504..3998)
                     /gene="lspA"
                     /locus_tag="ACHK3A_000840"
     CDS             complement(3504..3998)
                     /gene="lspA"
                     /locus_tag="ACHK3A_000840"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO8738968.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(3998..6814)
                     /gene="ileS"
                     /locus_tag="ACHK3A_000841"
     CDS             complement(3998..6814)
                     /gene="ileS"
                     /locus_tag="ACHK3A_000841"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO8738969.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSLSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCANAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(6857..7798)
                     /gene="ribF"
                     /locus_tag="ACHK3A_000842"
     CDS             complement(6857..7798)
                     /gene="ribF"
                     /locus_tag="ACHK3A_000842"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO8738970.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(7806..8024)
                     /gene="yaaY"
                     /locus_tag="ACHK3A_000843"
     CDS             complement(7806..8024)
                     /gene="yaaY"
                     /locus_tag="ACHK3A_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO8738971.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            8127..8390
                     /gene="rpsT"
                     /locus_tag="ACHK3A_000844"
     CDS             8127..8390
                     /gene="rpsT"
                     /locus_tag="ACHK3A_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO8738972.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            complement(8486..9391)
                     /gene="nhaR"
                     /locus_tag="ACHK3A_000845"
     CDS             complement(8486..9391)
                     /gene="nhaR"
                     /locus_tag="ACHK3A_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO8738973.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(9451..10617)
                     /gene="nhaA"
                     /locus_tag="ACHK3A_000846"
     CDS             complement(9451..10617)
                     /gene="nhaA"
                     /locus_tag="ACHK3A_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO8738974.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNHADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            11203..11355
                     /gene="mokC"
                     /locus_tag="ACHK3A_000847"
     CDS             11203..11355
                     /gene="mokC"
                     /locus_tag="ACHK3A_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO8738975.1"
                     /translation="MKQPKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(11459..12589)
                     /gene="dnaJ"
                     /locus_tag="ACHK3A_000848"
     CDS             complement(11459..12589)
                     /gene="dnaJ"
                     /locus_tag="ACHK3A_000848"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO8738976.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(12678..14594)
                     /gene="dnaK"
                     /locus_tag="ACHK3A_000849"
     CDS             complement(12678..14594)
                     /gene="dnaK"
                     /locus_tag="ACHK3A_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO8738977.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEVGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            14970..15374
                     /gene="yaaI"
                     /locus_tag="ACHK3A_000850"
     CDS             14970..15374
                     /gene="yaaI"
                     /locus_tag="ACHK3A_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO8738978.1"
                     /translation="MKSVFTLSASLAISLLLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            15400..16113
                     /gene="msyB"
                     /locus_tag="ACHK3A_000851"
     CDS             15400..16113
                     /gene="msyB"
                     /locus_tag="ACHK3A_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO8738979.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            16262..16828
                     /gene="satP"
                     /locus_tag="ACHK3A_000852"
     CDS             16262..16828
                     /gene="satP"
                     /locus_tag="ACHK3A_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO8738980.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(16863..17450)
                     /gene="mog"
                     /locus_tag="ACHK3A_000853"
     CDS             complement(16863..17450)
                     /gene="mog"
                     /locus_tag="ACHK3A_000853"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO8738981.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYIETAPEVVAAFRPKSARRDVSE"
     gene            complement(17565..18518)
                     /gene="tal"
                     /locus_tag="ACHK3A_000854"
     CDS             complement(17565..18518)
                     /gene="tal"
                     /locus_tag="ACHK3A_000854"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO8738982.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            18797..20227
                     /gene="yaaJ"
                     /locus_tag="ACHK3A_000855"
     CDS             18797..20227
                     /gene="yaaJ"
                     /locus_tag="ACHK3A_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO8738983.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            20297..21073
                     /gene="yaaA"
                     /locus_tag="ACHK3A_000856"
     CDS             20297..21073
                     /gene="yaaA"
                     /locus_tag="ACHK3A_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO8738984.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(21113..21409)
                     /locus_tag="ACHK3A_000857"
     CDS             complement(21113..21409)
                     /locus_tag="ACHK3A_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO8738985.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEMTLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(21623..22909)
                     /gene="thrC"
                     /locus_tag="ACHK3A_000858"
     CDS             complement(21623..22909)
                     /gene="thrC"
                     /locus_tag="ACHK3A_000858"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO8738986.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFVFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLHPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(22910..23842)
                     /gene="thrB"
                     /locus_tag="ACHK3A_000859"
     CDS             complement(22910..23842)
                     /gene="thrB"
                     /locus_tag="ACHK3A_000859"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO8738987.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(23844..26306)
                     /gene="thrA"
                     /locus_tag="ACHK3A_000860"
     CDS             complement(23844..26306)
                     /gene="thrA"
                     /locus_tag="ACHK3A_000860"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO8738988.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(26332..26451)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(26387..26452)
                     /gene="thrL"
                     /locus_tag="ACHK3A_000861"
     CDS             complement(26387..26452)
                     /gene="thrL"
                     /locus_tag="ACHK3A_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO8738989.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(26666..27352)
                     /gene="yjtD"
                     /locus_tag="ACHK3A_000862"
     CDS             complement(26666..27352)
                     /gene="yjtD"
                     /locus_tag="ACHK3A_000862"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO8738990.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADKHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(27752..27892)
                     /gene="yjjY"
                     /locus_tag="ACHK3A_000863"
     CDS             complement(27752..27892)
                     /gene="yjjY"
                     /locus_tag="ACHK3A_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO8738991.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            27988..28704
                     /gene="arcA"
                     /locus_tag="ACHK3A_000864"
     CDS             27988..28704
                     /gene="arcA"
                     /locus_tag="ACHK3A_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO8738992.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(28763..30115)
                     /gene="creD"
                     /locus_tag="ACHK3A_000865"
     CDS             complement(28763..30115)
                     /gene="creD"
                     /locus_tag="ACHK3A_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO8738993.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAHWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFIAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGAIMNGIYLQAVLKGWCNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(30173..31597)
                     /gene="creC"
                     /locus_tag="ACHK3A_000866"
     CDS             complement(30173..31597)
                     /gene="creC"
                     /locus_tag="ACHK3A_000866"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO8738994.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDTTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPTDPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVEQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(31597..32286)
                     /gene="creB"
                     /locus_tag="ACHK3A_000867"
     CDS             complement(31597..32286)
                     /gene="creB"
                     /locus_tag="ACHK3A_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO8738995.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLTLHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(32299..32772)
                     /gene="creA"
                     /locus_tag="ACHK3A_000868"
     CDS             complement(32299..32772)
                     /gene="creA"
                     /locus_tag="ACHK3A_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO8738996.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            32983..33852
                     /gene="robA"
                     /locus_tag="ACHK3A_000869"
     CDS             32983..33852
                     /gene="robA"
                     /locus_tag="ACHK3A_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO8738997.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(33849..34496)
                     /gene="gpmB"
                     /locus_tag="ACHK3A_000870"
     CDS             complement(33849..34496)
                     /gene="gpmB"
                     /locus_tag="ACHK3A_000870"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO8738998.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            34548..35063
                     /gene="yjjX"
                     /locus_tag="ACHK3A_000871"
     CDS             34548..35063
                     /gene="yjjX"
                     /locus_tag="ACHK3A_000871"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO8738999.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            complement(35057..35383)
                     /gene="trpR"
                     /locus_tag="ACHK3A_000872"
     CDS             complement(35057..35383)
                     /gene="trpR"
                     /locus_tag="ACHK3A_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO8739000.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(35473..37410)
                     /gene="sltY"
                     /locus_tag="ACHK3A_000873"
     CDS             complement(35473..37410)
                     /gene="sltY"
                     /locus_tag="ACHK3A_000873"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO8739001.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWELSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            37746..39413
                     /gene="ettA"
                     /locus_tag="ACHK3A_000874"
     CDS             37746..39413
                     /gene="ettA"
                     /locus_tag="ACHK3A_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO8739002.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(39469..39753)
                     /gene="nadS"
                     /locus_tag="ACHK3A_000875"
     CDS             complement(39469..39753)
                     /gene="nadS"
                     /locus_tag="ACHK3A_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO8739003.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPDFFRELASH"
     gene            complement(39755..40087)
                     /locus_tag="ACHK3A_000876"
     CDS             complement(39755..40087)
                     /locus_tag="ACHK3A_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8739004.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(40179..41411)
                     /gene="nadR"
                     /locus_tag="ACHK3A_000877"
     CDS             complement(40179..41411)
                     /gene="nadR"
                     /locus_tag="ACHK3A_000877"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO8739005.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(41432..42814)
                     /gene="radA"
                     /locus_tag="ACHK3A_000878"
     CDS             complement(41432..42814)
                     /gene="radA"
                     /locus_tag="ACHK3A_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO8739006.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(42863..43831)
                     /gene="serB"
                     /locus_tag="ACHK3A_000879"
     CDS             complement(42863..43831)
                     /gene="serB"
                     /locus_tag="ACHK3A_000879"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO8739007.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            43937..44581
                     /gene="ytjB"
                     /locus_tag="ACHK3A_000880"
     CDS             43937..44581
                     /gene="ytjB"
                     /locus_tag="ACHK3A_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO8739008.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            44609..45625
                     /gene="lplA"
                     /locus_tag="ACHK3A_000881"
     CDS             44609..45625
                     /gene="lplA"
                     /locus_tag="ACHK3A_000881"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO8739009.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            45657..45920
                     /locus_tag="ACHK3A_000882"
     CDS             45657..45920
                     /locus_tag="ACHK3A_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8739010.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(46081..46800)
                     /gene="deoD"
                     /locus_tag="ACHK3A_000883"
     CDS             complement(46081..46800)
                     /gene="deoD"
                     /locus_tag="ACHK3A_000883"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO8739011.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(46857..48080)
                     /gene="deoB"
                     /locus_tag="ACHK3A_000884"
     CDS             complement(46857..48080)
                     /gene="deoB"
                     /locus_tag="ACHK3A_000884"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO8739012.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(48132..49454)
                     /gene="deoA"
                     /locus_tag="ACHK3A_000885"
     CDS             complement(48132..49454)
                     /gene="deoA"
                     /locus_tag="ACHK3A_000885"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO8739013.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(49532..50311)
                     /gene="deoC"
                     /locus_tag="ACHK3A_000886"
     CDS             complement(49532..50311)
                     /gene="deoC"
                     /locus_tag="ACHK3A_000886"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO8739014.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            50569..52119
                     /gene="yjjI"
                     /locus_tag="ACHK3A_000887"
     CDS             50569..52119
                     /gene="yjjI"
                     /locus_tag="ACHK3A_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO8739015.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            52091..52954
                     /gene="yjjW"
                     /locus_tag="ACHK3A_000888"
     CDS             52091..52954
                     /gene="yjjW"
                     /locus_tag="ACHK3A_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO8739016.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALWERG
                     VSRLIFPALYL"
     gene            complement(53172..53951)
                     /gene="yjjV"
                     /locus_tag="ACHK3A_000889"
     CDS             complement(53172..53951)
                     /gene="yjjV"
                     /locus_tag="ACHK3A_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8739017.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLKKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYTLFNVP"
     gene            complement(53948..55021)
                     /gene="yjjU"
                     /locus_tag="ACHK3A_000890"
     CDS             complement(53948..55021)
                     /gene="yjjU"
                     /locus_tag="ACHK3A_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO8739018.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(55143..55304)
                     /gene="ytjA"
                     /locus_tag="ACHK3A_000891"
     CDS             complement(55143..55304)
                     /gene="ytjA"
                     /locus_tag="ACHK3A_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO8739019.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(55431..56036)
                     /gene="osmY"
                     /locus_tag="ACHK3A_000892"
     CDS             complement(55431..56036)
                     /gene="osmY"
                     /locus_tag="ACHK3A_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO8739020.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(56429..58018)
                     /gene="prfC"
                     /locus_tag="ACHK3A_000893"
     CDS             complement(56429..58018)
                     /gene="prfC"
                     /locus_tag="ACHK3A_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO8739021.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQSAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(58109..58786)
                     /gene="yjjG"
                     /locus_tag="ACHK3A_000894"
     CDS             complement(58109..58786)
                     /gene="yjjG"
                     /locus_tag="ACHK3A_000894"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO8739022.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(58801..59247)
                     /gene="rimI"
                     /locus_tag="ACHK3A_000895"
     CDS             complement(58801..59247)
                     /gene="rimI"
                     /locus_tag="ACHK3A_000895"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO8739023.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(59216..59629)
                     /gene="holD"
                     /locus_tag="ACHK3A_000896"
     CDS             complement(59216..59629)
                     /gene="holD"
                     /locus_tag="ACHK3A_000896"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO8739024.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPIVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            59732..60763
                     /gene="rsmC"
                     /locus_tag="ACHK3A_000897"
     CDS             59732..60763
                     /gene="rsmC"
                     /locus_tag="ACHK3A_000897"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO8739025.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLSDYAPLNKMDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            60963..61049
                     /locus_tag="ACHK3A_000898"
     tRNA            60963..61049
                     /locus_tag="ACHK3A_000898"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60997..60999,aa:Leu,seq:cag)
BASE COUNT        14528 a        16633 c        15285 g        14650 t
ORIGIN      
        1 tttgcatcac tataaatatt ttgtggttac agattattga gatcaagcac atctcgcata
       61 tcaaaaagac cactttcctt accactcaac cacaaagccg atcttaccgc accgttagca
      121 aatgtcatgc ggctggacgc cttatgggtg atctccagac gctcgccaat atcggcaaac
      181 atcgcggtat gttcaccaac gatgtcacct gcacgcacgg tggcaaaacc aatggtgcca
      241 ggcacacgtt caccggtatg gccttcacga ctgtagaccg cgcaatcttt cagatcttta
      301 tccagagcgt gggcgatcgc ctctcccatt gccagtgcgg tgcctgacgg cgcatcaact
      361 ttatgtctat gatgtgcttc aataatttcg atatcggtgt agtcacccat cactttggct
      421 gctttctcca gcagtttaag catgacgtta acgccaacgc taaaattggc ggcaaagaca
      481 atcgcaatat cggcggcggc gtcacgaatc gcctgtttac cggcttcgtc aaaccccgta
      541 gtgccgatca ccatcccttt gccatgctgg cgacaaaaag cgagatggtt cagcgtacct
      601 tccggacggg taaaatcgat aaacacatca aaatcatctt ttatcgcatc aagactactt
      661 tgcacagtaa cgcctgtttt cccggctccg gccagttcgc ccgcatcgct gcccagtaaa
      721 gaagatcctt cgcgctccag cgccgcgccc agctgcacgc cttctaatgc cagcgccgcc
      781 tgaatcaact ggcggcccat acggcccccg gctcccgcga tggcaacgcg gatgtttgca
      841 tcatgcatag ctattctctt ttgttaattt gcatagaccg ttttcagagt aaccagcgca
      901 tcgctacgcc gccagccaaa aacgctgata attagaaatt aatgataaac agggagggat
      961 atcagtgaaa ggcatgagtc gatgaatgac tgcatgccga taacatgtga caggttacgg
     1021 caccagagcc agcacctcag ctacccactg ctggaagcct tttacattca gatccaatgc
     1081 cacctgtaca ttggctggtt tgcccaggca accgtcgata tcaaccaccg tcgtgccaga
     1141 ggtaaattcg ccctgcgttt ccactgccac aaaacacggc ttgagagtga acaggtccgg
     1201 gcgcaccagc caggcgatgg cgcagagatc gtgcattcgc agtccacttt gcatactgcc
     1261 gctacggtag tgactaaaca gggcgtggag catttttccg gtgcgattca actccggcaa
     1321 ggtagcgaga tagtcagggg ttaatattgc ctgattggtg acatccagac cgcacatgac
     1381 gatttcgatg ccgctacgga agacacaggc tgccgcttct ggatcggcag cgatattaaa
     1441 ctcggcgttt ggtgtgcagt tgccgcgacc agcagaaccg cccatgatca ccagacggcg
     1501 aatatacggc ttacattccg ggcattgtga aagtaacagc gcaatattgg ttaacgggcc
     1561 gatggccacc agggtaacgg gctctggcgc acgcatcagg gcatcacgaa ttgccagaaa
     1621 cgccggtata cccagtggct gacggttgtg ttcaacaaag tcatagccag ccattcccga
     1681 ttcaccgtgt acagatgccg catcgcgcgg cgcacgaacc agtggtacgg cagcgccctg
     1741 ggcaagcgga atctccgcat tccagaaatg cagcagttgc agggcgttgc gggtcgtttt
     1801 ctctaccgaa acattacccg cgacagtggt catcagttgc agatcgagtt cgggggtaaa
     1861 aatcgcggcg gcaatggcaa cggcatcgtc aatgccgggg tctgtatcga ggaagatagg
     1921 taaacgcatg ttttctccat aaaaaatgcc ggtaacaaga ccggcatttt cgcataactt
     1981 aggctgctaa tgacttaatc gacttcacga atatcgacac gcagctcttt cggcacttcg
     2041 aaaacaatgt tttcttcacg accttccagc ggaatggctt caccaccgcc cagctgttgc
     2101 aaacgtgcca ccacattctg caccagaata tccggagccg atgcgcccgc agtcacgcca
     2161 acgcagttca cgccttttac ccactcttcc tggatatctt tcgcatcgtc aatcaaaaat
     2221 gcgcgtttgc ccatacgctg ggccagttcc gccagacggt tggagttgga ggagtttttc
     2281 gaaccaacca ccagtactac ttccgcctgc tctgccaggg cgcgtaccgc ctcctgacgg
     2341 ttagtcgtgg cgtagcagat gtcatctttg cgcggaccga caattttcgg gaagcgttta
     2401 cgcagcgcgt cgatcacatc agacgtgtca tccaccgaca gcgtggtttg ggtcataaag
     2461 gagagcttct cttcgttttt gaccgtcagt ttccacacat cgtctggcga ttcgaccaga
     2521 tacattcccc cttccgggtt gctgtactgg cccatcgtcc cttccacttc cgggtgcccg
     2581 gcgtgaccga tgagaataga ttcttcacca cgacggctgg cgcgggcgac ttccatatgc
     2641 actttggtca ccagcggaca ggtggcatca aacaccgtca aatcgcggct tttcgcttcg
     2701 ttacgtactg cctgagaaac accgtgtgcg gagaaaatca ggatcgcgcc gtccggtact
     2761 tcgctaatct gctcaataaa gatagcccca cgctcgcgca agctatcgac cacgtagcgg
     2821 ttatgcacca cttcgtgacg gacatatatc ggtgcgccgt aaatggccag cgcgttttca
     2881 acaatgctga tagcgcggtc taccccggca caaaaaccac gcgggttggc caacaggatc
     2941 tgcatgttac gcctccagtg ccggatcgat ttccagcact tcaatatcaa aatgaacggt
     3001 ctgcccggcc agcggatggt tgaaatcaac ggtaatggag tcgccgttaa tttcgcggat
     3061 cacgccaggc atctcactgc catccattgc ggtaaaaagc atgattgcgc caatttctgg
     3121 ctcgcctgca tccataaatt cacggcggga gaagtactga atcaggtccg gtgacggcac
     3181 gccaaacgcc gcatcaggct ccagcgagaa ggtggtttta tcgcccactt tcagccccag
     3241 caggtgttgc tccagccctt cagaaagaga agcatcaccc aggcggaaca gcgccggttt
     3301 accgttgttg cgggtagact cagcggtggt gccatcatcg agtttcagcg tgaagtgcac
     3361 caggacggcg ctattgctct gtacagattc agacatgcag gtttgctctt atattttgag
     3421 gatttttgcc ggatgcggcc tacgatttcg cagcaatctg taggccggat aagatgcgtc
     3481 agcatcgcat ccggcagggt ttattattgt tttttcgctt tagaaggtaa aaaaccttcc
     3541 agcacaatca gtgccgcacc gacacaaatg gcagtatcgg caaggttgaa ggtggcgaag
     3601 tgccagtcgc cgacatagaa gtcgatcata tcgacaacga agccgtgcca caggcggtcg
     3661 aacaggttgc ccagcgcgcc gccaataatc agcgcgtaag cgatattgtt tagcttctgc
     3721 gtggccttcg agcgatacat catcactgcc aggatcacgc taataccaat cgcaataccg
     3781 gcaaaaaacc aacgctgcca gccgccacta tcggcaagga aactaaacgc cgcgccatag
     3841 ttacgcgcat aatgcagatt aagcgacggg aacagcggga ccgtatcccc cagagcaaag
     3901 ttctggagga tcaggtattt gctgcccaga tcgataatca gcacgactac caccagccac
     3961 agccagcgta gccctgttga acagatcgat tgactcatca ggcaaactta cgtttttcac
     4021 cgtcaccggc gacgttgctg acacaacggc cgcagatttc tgcgtgttcc gccaccttgc
     4081 cgacatcctg ggtgtagtgc cagcagcgtg ggcacttctc accttcggct ttactcaacg
     4141 cgactttcag ccctttgagg acttcgctct gctgggcgtc agcaggtgcg tcgttatagt
     4201 ctgcaacggt agcgccggag gtcaacagga caaatcgtaa ttcatcgccc agcgcggtca
     4261 gtttcgccgc cagttccggt tctgcataca aggttactgc cgcttccagc gagccgccca
     4321 ctttcttatc ggcacgcgct tgctcaatga ctttgttcac ttcgccacgc actttcaaca
     4381 gctcgtccca gaacgcatcg ttcattgctt cactgtctgc cagaccaaac aggccttcgt
     4441 accactcacc ggtgaagacg tatttttcac gttcgcccgg caggtaaccc cacacttcat
     4501 cagcggtgaa ggagaggatt ggtgccatcc agcgaaccag cgcttctgcg atgtgataca
     4561 gcgcagtctg gcagctacga cgcgccacgc tgtccgcttt ggcggtatac tgacggtctt
     4621 tgatgatgtc gaggtagaag gaccccatct caacggagca gaagcgcatc agacgctgta
     4681 ccacttcgtg gaaatcgtat gcttcgtacg ctttgaggat gtcttcctgt gccgcgttcg
     4741 cacaacctac ggcccagcga tccagtacca ccatctcttc cggtttcacc atatcttttg
     4801 ccggatcaaa accgttcagg tttgccagca ggaagcgcgc ggtgttacgg atacgacgat
     4861 agctgtcggc agcacgtttc aggatctcgt cagaaacggc catttcgcca gtgtagtcgg
     4921 ttgatgccac ccacagacgc agaatatccg cacccagttt gttcatcaca tcctgcggcg
     4981 aaacggtgtt accgatggat ttagacatct tgcgaccctg accatccacg gtaaagccgt
     5041 gagtcagtac ctgacgatat ggcgctttgc ccttcatcgc ggtagagatc atcagggaag
     5101 acatgaacca gccacggtgt tggtcagaac cttccagata catgtccgct gcgtgaccgg
     5161 caaattccgg acgcacatca acaacggaag agtgggtaga tccggagtca aaccatacat
     5221 ccagcgtatc cggtactttc acatactggt cagcttcgtc gccgaggatc tctttcgcat
     5281 cgagatccca ccacgcctga atgccgtcaa cttcaacgcg ttttgccact tcttccatca
     5341 gttcgagagt acgcggatgc agttcttctg tgtctttgtg cacgaacagt gacatcggca
     5401 cgccccaggt acgctgacga gagatacacc agtcaggacg gttagcaacc atcgactcga
     5461 tacgcgcctg gccccagtca gggatccact gcacgccttt gatctctttc agtgactgcg
     5521 cacgcagacc tttctgatcc atgctgacga accactgcgg cgtcgcgcgg aagatgatcg
     5581 gcgttttatg acgccagcag cacggatagc tgtgttgcat tttctcaacg tgcaacagtg
     5641 cgcctttttc ctgcaacaac gcaatgacaa tatcgttcgc tttgaagacg ttaacgccat
     5701 ccagagtcgg gtaagtaccc ggcagataag tgccgtccgg gccaaccggg ttagcggttt
     5761 ccagaccata tttttgaccg atcacatagt cgtccggacc gtggcctggc gcggtatgaa
     5821 cggcaccggt accggcatcc agcgtaacgt ggtcgccgag aattgccgga acatcgaagt
     5881 ccataaacgg atgggtaaag cgcaacagtt ccagctcggc acctttcacc gtgccaagaa
     5941 tggtgtaatc gctaacgccg atacgctgca ttacgctttc aaccagatct ttcgcgagga
     6001 tcacggcctg accgtcgatt tgcaccagcg cataatcaaa atcaggtgcg atggagattg
     6061 cgcggttagc aggcagcgtc cacggcgtgg tggtccatat aaccagcgaa attgggccgt
     6121 taacattgct tacgccaaat ttcgttttaa gcgcatcctg atcgaccgcc tggaaagcga
     6181 cgtcgatgga cagagaagtt ttgtcgtaat actcaacttc cgcttctgcc agtgcagaac
     6241 ggcagtcaac gcaccagtgc accggcttcg cgcctttgtg caggtgaccg ttgccgatga
     6301 ttttgcccag cgcgcggatg atgttggctt cagttttgaa gtccatggtc aggtacgggt
     6361 gcgaccagtc gcccagcacg cccagacgga taaagtcttt gcgctgaccg tcaacctggg
     6421 tcgcagcgta ttcgcggcac ttggcgcgga actcagcggc ggtgaatttc tcccccggct
     6481 taccgtattc ttgctctact ttcagctcga ttggcagacc atgacagtcc cagccaggca
     6541 catacggcga gtcatatcca gaaagccctt tggacttaat gataatgtct ttcagaatct
     6601 tgttaaccga gtgaccaata tgaatactgc cattcgcata aggagggcca tcatgcagaa
     6661 tgaaggtttt tttgcctttt ttagctgcac gaatgatgcc gtacagatca tcatcagtcc
     6721 aacgcgccag cattcccggt tcgcgcttgg cgagatcgcc acgcatcggg aaccctgttt
     6781 ccggcaaatt cagggttgat ttatagtcac tcatcagatt ctcggttccg tatttcggtt
     6841 tgattacata acaggcttaa gccggttttg ttagcccaaa aaattcgcgg gcggttaatt
     6901 catcacgcgc aatctgcgct ttcagttcgt ccagcgatgc aaatcgctgc tcattgcgta
     6961 tttttttacg cagcactact tgtatatggc gaccataaag gtccattgca acatctaaca
     7021 aatgcacttc cagttgctgg cgaataccgg caaccgttgg gcgtgttccg atgtttgcaa
     7081 cgccgggtaa cggcttttcg ccaaggccca acacttctac cgcataaacc cctttcaccg
     7141 gggaaacctg acggcgtagc ggtacattcg ccgtcgggaa acctatagtg cgccctaatt
     7201 catcaccgtg gactacacgc ccggagatag caaacgggtg ccccagtaaa ctttctgcca
     7261 gagccagatt gtcatccgca agcgcctgac gcacggcggt gctgctgata cgcacgccac
     7321 cttcgcaaaa agtttgcgtg ctggtgatat cgaagccgta ttccatgccc gctttctgta
     7381 ataacaagaa atcgccttca cgaccagcgc caaagcggaa atcgtcacct acggcaagaa
     7441 attttacccg caagtgcttc accagaagat cactgatgaa gttttgcgcg gttaacgccg
     7501 caaaacgcct gtcaaaacgc acgcacagca cgtaatcaac accacactct gcaagataac
     7561 gcagtttttc ccgcagccgg gtgagccgtg ccggggcttt atcagtagca aacagttcca
     7621 gtggttgagg ttcaaaaagc atcaccatca ccggtaagtt gcgcttgcgc ccttcttcct
     7681 gcaagccctg taacagcgcg cgatgaccgc gatgcacgcc gtcgaaatta ccaatagtca
     7741 gcacacaccc ttcttgcggg gcctggctga gattatgtat gccgcgtatc agcttcatgt
     7801 ctggctcaaa acagtgaaaa tcgtccgagt ataccttgta cagcggtcaa ggttaaccgg
     7861 cgattgagta ccgaaatcag aaagaagtgg cttttcattg ctatggcgca aatcatggga
     7921 agaaactgac cgcctgctgc aatttttctc gcggaaaagc tgtattcaca ccccgcaagc
     7981 tggtagaatc ctgcgccatc actacgtaac gagtgccggc acattaacgg cgcttatttg
     8041 cacaaatcca ttgacaaaag aaggctaaaa gggcatattc ctcggccttt gaattgtcca
     8101 tatagaacac atttgggagt tggaccttgg ctaatatcaa atcagctaag aagcgcgcca
     8161 ttcagtctga aaaggctcgt aagcacaacg caagccgtcg ctctatgatg cgtactttca
     8221 tcaagaaagt atacgcagct atcgaagctg gcgacaaagc tgctgcacag aaagcattta
     8281 acgaaatgca accaatcgtg gaccgtcagg ctgctaaagg tctgatccac aaaaacaaag
     8341 ctgcacgtca taaggctaac ctgactgcac agatcaacaa actggcttaa ttgcctgttg
     8401 ttgtcagctt tgtaaaaaaa cccgctctgg cgggtttttt tatatccatc tgcaggccaa
     8461 aattcggcct gcaaagtaca aatgtttatt ttgaagctgg agtaaacagc gcagaatagt
     8521 ctgtattgca gatacgctgt actgcagggt gctgaatcat cctttcggca aaaatcgcgt
     8581 ggtactcttc catcacgttc tcaacacggc ctatctccac aaccgagtca tcgttataga
     8641 aatcattagc gtaaagcgaa ggtgcaacga aaatagcgtt atgcgtcgcc ccaaaggctt
     8701 tcatcaacgc agcatcatca aactcaccca aaatttcgac gttcaagccc tgggagttaa
     8761 accagtttaa tagcttacgc cccaacattg agcgacgccc cggaataagt aaacgacgct
     8821 cttcaagaca ggcaggaaac ggcttttctg gtagtgggtt agtgcaccag aaactgacac
     8881 cacattcgcc aattttcatg gaaaacagcc cttcctgctg agtggaatcg atcggacagt
     8941 cagagatgat catatccagt ttatgctgac tcaactgctc taaaagcatc tcgtgcgtcg
     9001 attcgaaaca gcgtagatgg atctgctcgt cttccacaac tgcggcatcc agaacactgc
     9061 tgaccagacg tttggaaagt gcatctgcca caccaacatc aaacaataag ttggactctt
     9121 tgcgatagtt gacgatatcc agcatttcct ggcttaaggt gaacatttta tcggcatagc
     9181 gatagaccag ctctcccagc tcgctgggct cgaggccacg tcccttgcgc ttaaataatt
     9241 tgccttgcag gcgctcttcc agcgcccgaa tctgtccggt aatggtttgt ggtgttaaat
     9301 aaagcgcctc cgctgcgcca accacggaac cttctttata gacatgccag aagtaataca
     9361 agtggttgta attgatatga gacatgctca tttctctccc tgataacaat gaaaagggag
     9421 ccgtttatgg ctccccggta aaccgtcctg tcaaactgat ggacgcaaac gaacgcgtaa
     9481 ccagctgtat ccaattaccg ccgaagagat agaaccgacc aggataccga gtttcgccca
     9541 gttaatcagt tctggatcta cgctaccaaa ggccaggctg gcaataaaga tagacatagt
     9601 aaaaccgata ccgcacagga tccccaccgc cataatttgc tgataagtcg ttccctcagg
     9661 cagatgcgcc agtttcaaac gcagcgccaa ccagcagaac agactaatcc ccagcggttt
     9721 gccaatcagc aagccagcga tgatccccaa tggcagaatg gaggtcaagc catccagcgt
     9781 gacgccttgc agtgaaacgc cagcattagc aaatgcaaac agcggtaaaa tcagatacgc
     9841 cacccacggg tgcaacacat gctccagtcg cttcgctgga gaacgcccat gcttctcttt
     9901 caaaggaata aagaagccga caattacccc cgccagggtt gcgtgaaccc ccgatttcaa
     9961 caccgcagtc cacaacacca cgccaaccag aatatagacg cccgtgcggc gtacaccaca
    10021 cagattcaat accgcgagta ccgcaattgc tacagccgcg acgccaagag aggccatcga
    10081 taagtcatta gtgtagaaca atgcgatgat aatgatggcc ccaagatcgt cgataatagc
    10141 cagagccatc aaaaagatct tcagggctaa cggaacacga cttcccaaca gcgccagcac
    10201 accaagtgca aaagcaatgt cagtagccgc cgggatcgcc cacccttcgc gggtaatcgg
    10261 atcggcatgg ttaaaggcca gatagagtaa tgccggcaca atcatcccac caatagcggc
    10321 gataactgga aatgcagcct ggcgtaagct ggctagcgat ccttgcatca gttcacgttt
    10381 aacttccaga ccgactaaca ggaaaaatac cgccatcagc gcgtcattta tccataacag
    10441 catgtttttg ttgatttcga gtgaaccaac ccggagctga accggcgtct ccagaaagtc
    10501 gtgataccat ccactggttg cgccgctgtt ggccataatc atcgccagga tagcggcaat
    10561 gataagaata atgcctcccg aggcatcact gctaaagaat cgatgcagat gtttcacttt
    10621 ttatttctct ttcaggtgaa tagatcgttt ttactatctt accccgcacg aatcatcgtt
    10681 aaaatcagat tatagtgtag gaataattcg ctttttacga ggcattgaag gatttgcgtc
    10741 atcagtgatg agatgagttt ttgacggctg taagcgctga cgtgtgaccc taagccctga
    10801 taatcatagc ttaaggtcag gatttctgag ggattaatta ggaaaagtaa taattaattt
    10861 gttgtcagtc ggcgatgttt gttaggccaa tacttcaaaa ataatccagt ttgtcgtcaa
    10921 taattgcgat gaagagataa attaaagtta aataattaaa tcattaaaaa attacgccat
    10981 aaaaatgcga ggcgtcttca ggattagaat cgataaaaca gaatgggtta aagagagcga
    11041 tataatagcg gcgggtgctt gaggctgtct gtctcaggca ttagctgaac ggcagataga
    11101 gaaaagcccc gagtgatatt ttactatcaa cccgaggccc cctatatgca gaacacatgt
    11161 agagtgcctc ttactgaccg taaggtcaag gagaagagag caatgaagca gcctaaggcg
    11221 atgattgtcg ccctgatcgt catctgtatc accgccgtag tggcggcgct ggtaacgaga
    11281 aaagacctct gtgaggttca catccgaact ggccagacgg aggttgctgt tttcacggct
    11341 tacgaatccg agtaagagca acggcgggga gtgatccccg ccacttttca ggtgctcgca
    11401 tatcttcaac gcaccctatt tttacccagg cctgcccacg ggcaggcttt tggggagatt
    11461 agcgggtcag gtcgtcaaaa aacttcttca caccatcaaa gaagctcttt gagcgcgggc
    11521 tgttgtgctc gccggttggg ccaccgaagc tttcttgcag ctcttgcagc agctgtttct
    11581 gcttctcgtt caggcctacc ggtgtttcga cgacaacgcg gcacagcaaa tcaccctgtg
    11641 cgccaccgcg gacagacttg acgcctttac cgcgcatacg gaacagctta ccggtctggg
    11701 tttcgccagg cactttcagt ttgacgcgac catcaagggt cggtacttcg atttcgccac
    11761 ccagcgccgc catagcgaag ttgatcggga cttcgcaata gaggttgttg ccttcacgct
    11821 cgaaaatcgg gtgctgttta acctgaacct gaacgtacag atcgcctgcc ggtgcgccat
    11881 gctcgcccgc ttcaccttcg cccgcaagac ggatgcggtc tccagtgtcc acccctgccg
    11941 ggattttaac ggacagcgtt ttgctgcgct caacacgacc atgaccatga catttgttgc
    12001 acggatcttt gatcagcgta ccgcggccct gacagtgtgg acaggtctgc tgcacggcaa
    12061 agaaaccctg gcgcatctgc acctgaccag aaccatgaca ggtcggacag gtctgcggct
    12121 gtgtacctgg ttttgcaccg ctaccgtggc aaacgtcaca ctcttccaga gtcggaatgc
    12181 ggatctcttt ggtcacgcca cgtacagctt cttcgagggt gagctccatg ttatagcgta
    12241 aatcagcacc gcgcgccgca cgttgacgac cacgtccgcc gccaaaaata tcgccgaaaa
    12301 cgtcaccaaa aatatcgctg aagtctgcgc cgccgccgaa accgccgcct cccatgccac
    12361 cttgctcaaa cgcagcatga ccatactgat cgtatgccgc acgtttttgc gagtcggtca
    12421 gaacttcata agcttccttg atctctttaa atttcgcctc ggcctctttg tcaccctggt
    12481 tacggtccgg gtggtatttc atggccaggc gtttgtaggc ctttttgatt tcacgctctt
    12541 ccgctgtttt ggaaacgcct aaaatctcgt aataatcttg cttagccatc ttttttaaat
    12601 tgcccctaga tgaatgcacg ggcggagagg aaattcccct tcgcccgtgt cagtataatt
    12661 acccgtttat agggcgatta ttttttgtct ttgacttctt caaattcagc gtcgacaaca
    12721 tcgtcatctt tcgcgttgtt tgcagaagca tcagcaccgg cagtctgctg ctgggcatgt
    12781 tgctgctggg cgatttccat cagtttctgg gaaacctgtg ccagttcctg cattttcgct
    12841 tcgatagcgg ctttgtcttc acctttcaga gcagtttcca gtgcagtcag tgcagactcg
    12901 atagcagttt tgtcgtcagc cggcagtttg tcgcccactt cttcaacctg cttacgggtg
    12961 ctgtgcagca gatggtcgcc ctggttgcga gtctgtacca gctcttcaaa cttacggtca
    13021 gcttcggcgt tagcttctgc gtcgcgtacc attttctgga tttcatcttc gttcagacca
    13081 gaagaagcct tgatggtgat cttctgctct ttaccgctgt ttttatcttt cgcggaaacg
    13141 tgcaggatac cgtcagcatc gatatcgaag gtaacttcga tctgcggcat gccgcgcggt
    13201 gccgggttga taccatccag gttgaactga cccagagatt tgttatcagc tgcacgttta
    13261 cgttcaccct gcagcacatg gatggttacc gcagactggt tgtcttcagc ggtagagaac
    13321 acctggctgt gcttggtcgg gatagtggtg tttttcgcga tcagcgtcgt catcacaccg
    13381 cccatggttt cgatacccag agacagcggg gtaacgtcca gcagcagtac gtcttttacg
    13441 tcaccagtca gaacaccacc ctgaacagca gcaccgattg ctacagcttc gtccgggtta
    13501 acgtctttac gcggctcttt accaaagaac tcagcaactt tcttctgaac cattggcata
    13561 cgagtctgac caccaacgag gataacgtcg tcgatatcag atacggacag gccagcgtcc
    13621 tgcagtgcaa ctttcagcgg ctcaatggaa cggtttacca gatcttcaac caggctttcc
    13681 agtttcgcac gagtcacttt gatgttcatg tgtttcggac cggtcgcgtc tgcagtgatg
    13741 tacggcaggt taacgtcggt ctgctgagcg gaagacagtt cgattttcgc tttttctgcc
    13801 gcttctttca ggcgctgcat tgccagcgga tcgttgcgca ggtcaatgcc ctgatctttc
    13861 ttgaattctt caaccaggta gttgatcaga cggctgtcga agtcttcacc acccaggtgg
    13921 gtatcaccgt tggttgccag aacttcgaag gttttttcgc cgtcaacttc gtcgatttcg
    13981 ataatagaaa tatcgaaagt accaccaccc aggtcataaa ccgcgatagt acggttgcca
    14041 gtacctttgt ccagaccgta agccagcgca gctgcggtcg gttcgttgat gatacgtttt
    14101 acttccagac cagcgatacg gcctgcgtct ttggttgcct gacgctgagc atcgttaaag
    14161 tatgccggta cggtgataac agcttcagtt accggttcac ccaggtaatc ttcagcggtt
    14221 ttcttcattt ttttcagcac ttcagcagag atctgcggcg gtgccatttt ctggccttta
    14281 acttcgaccc atgcgtcgcc gttatcagca gcaataattt tgaacggcat gatggaaaca
    14341 tcacgctgta cttcttcgtc ctggaagcgg cggccaatca ggcgtttaat cgcaaacagg
    14401 gtgttttgcg ggttcgtcac tgcctgacgt ttagccggct gaccaaccag agtttcacca
    14461 tcctgggtat aggcaatgat agaaggcgtg gtgcgatcgc cttcggcgtt ctccagtaca
    14521 cgaggagtgg tgccatccat aatcgctaca caagagttgg tagtacccag gtcgatacca
    14581 attattttac ccatctaaac gtctccacta tatattcggt catcatgtgg ttgtgagtct
    14641 gtaatagggg cgaaacgtct ggtttcaact gcccaatttc atttttttgc agactcactc
    14701 actgcggttg actactaaat ggggtcgtaa cccacgtcat caagggggag atgcaaaaat
    14761 tttttgtgct tttgcgcaga aatttcgcga tatgccagaa caagacatga aactgacagc
    14821 aattttattc atatagagaa tgaatagcag acaagacatt catagaaaaa ttgtaataaa
    14881 aagaaaatat tacgtacctg aatcttctct ttatcagcag taaactagtg ggtattcatc
    14941 ccctacctct ccccactaag agaatcctta tgaaatccgt ttttacgctt tccgccagcc
    15001 tggcgattag cctgctgctg tgctgcacgg cgcaggcaaa cgaccataaa atcctcggcg
    15061 tcattgcaat gccacgtaac gaaaccaacg atctggcgct gaaacttcct gtttgccgaa
    15121 ttgttaaacg catacaactc tcagccgacc atggcgattt acagttaagc ggtgcatcgg
    15181 tttatttcaa agccgcccgt agcgccagtc agaccctgaa tattccttca gaaataaaag
    15241 aagggcaaac cactgactgg atcaacatta acagcgataa cgacaataaa cgctgcgtct
    15301 caaaaatcac cttttctggt catacggtga actcatcgga tatggccacg ctgaaaatta
    15361 tcggcgacga ctaacgccag aatatagaag ccacaaaaaa tgaatgttaa ttacctgaat
    15421 gattcagatc tggattttct ccagcattgt agtgaggaac aactggcaaa tttcgcccgg
    15481 ctgctcaccc ataatgaaaa aggcaaaact cgcctctcca gcgtactgat gcgcaacgaa
    15541 ctgtttaaat cgatggaagg acatcccgag caacaccgcc gcaactggca gctgattgcc
    15601 ggagaattac aacattttgg tggcgatagt atcgccaaca aactgcgcgg acacggtaaa
    15661 ttgtatcggg ccattttgct cgatgtttca aaacgattga agctgaaagc cgacaaagag
    15721 atgtctacgt ttgaaattga gcaacagtta ctggaacaat ttctgcgtaa tacctggaag
    15781 aaaatggacg aggaacataa gcaggagttt ctgcacgcgg tcgatgccag ggtgaatgag
    15841 ctggaagagc tgctgccgct gctgatgaaa gacaaattat tggcaaaagg cgtgtcgcat
    15901 ttgctttcca gccaattgac ccgcatttta cgcacccacg cagcaatgag cgtacttggg
    15961 catggtttgc ttcgcggcgc ggggctggga ggccctgtag gcgcggcact aaatggggtt
    16021 aaagcggtca gcggcagtgc ctatcgcgtg acaattccag ccgtactgca aatcgcctgc
    16081 ctgcgccgga tggttagtgc cactcaggtc tgataccgga aaccggggaa aattttcatt
    16141 ttcccctcaa aagatcgtag acactgcccc actggctgat tattatgccg cgccctgaaa
    16201 acactacagt tattcaggga aattatttca ccattcattc gatgatgatt tttgaggaat
    16261 tatgggcaac actaagttgg ctaatccggc accgctgggc ctgatgggct tcggcatgac
    16321 caccattctg cttaacctgc acaacgtggg ttattttgct ctggacggta ttattcttgc
    16381 catgggcatt ttctacggcg gcatcgcgca aatttttgct ggtctgctgg agtacaaaaa
    16441 aggcaacact ttcggtttaa ccgcattcac ctcttacggt tctttctggc tgacgctggt
    16501 tgcgattctg ctgatgccga aactgggtct gaccgatgcg ccaaatgcac agttccttgg
    16561 tgtctacctg ggtctgtggg gcgtatttac gctgtttatg ttcttcggca cgctgaaagg
    16621 cgcacgcgtt ctgcaattcg ttttctttag cctgaccgtg ctgtttgccc tgctggcgat
    16681 cggtaacatt gccggtaacg ccgcaatcat ccactttgcc ggctggattg gtctgatctg
    16741 cggtgccagc gcaatctatc tggcgatggg tgaagtactg aacgagcagt ttggtcgcac
    16801 cgttctgccg attggtgaat cccattaatt gttttgagat ccccccgctc ggggggattt
    16861 ttttattcgc taacgtcgcg tcttgcactc ttcggtctga atgctgcaac cacttccggt
    16921 gccgtttcaa tgtatggccc ttccagcaac tgaatgcagt acggtacgct ggcaaaaata
    16981 ccatgcacca caacgttacc cgcagcgtcc ttcacgcctt ccagcgtctc tttaatcgac
    17041 ttcggctgac cgggtaagtt aaggatcagc gcctgtttgc gaatcacgcc cacctgacgc
    17101 gaaaggatcg cagttggtac aaaatgcagg ctgatctggc gcatctgttc accaaagcca
    17161 ggcatctcgc ggtccgctac tgccagcgtc gcatcgggcg ttacgtcacg acgcgccggg
    17221 ccagttccgc ccgtggtgag caccagatgg caactcattt catccaccag ctcacacaac
    17281 gtttgctcga tgatcgcctg ctcatcgggg attaagcggg tttccagttc aaacggcgtg
    17341 gttagcgccg atgtcagcca ttcttccagc gcagggatgc ctttatcctg ataaacgccg
    17401 ctggatgcgc gatcagagat ggaaactaag ccaatacgta aagtattcat caagattttt
    17461 ccgtttaaac agtctcgtta aacagaatga tacactgcga agggagtgac agacaggctt
    17521 cagaaaaggt agcgtgaccg acttcccggt cacgctaaga atgattacag cagatcgccg
    17581 atcatttttt ccagtttttc ctggtcaaca gcaaacttac ggataccttc cgccagttta
    17641 tctactgcca ttggatcctg gttatgctgc cacaggaact cggactcagt gatacgcgcc
    17701 ggacgcgctt tcacttcgcc ggtgtaagac agtttacgtt cgatagcccc ttcgctctca
    17761 gccagctctt tcagcagcgc cggtgcgatg gtcagacggt cgcagcctgc cagttccaga
    17821 atttcgccga tgttacggaa gcttgcgccc atcaccacgg tttcataacc gtgctctttg
    17881 tagtactggt agatttcaga tacagaaacc acgcccggat cttctgccgg agcgtactct
    17941 ttcttgtcgg tattcgcttt gtaccagtca agaatacggc caacaaacgg cgagatcagg
    18001 aacacgcccg cttccgcaca agcacgagcc tgagcgaagg agaacagcag ggtcaggtta
    18061 cagttaatac cttctttttc cagctgttct gcagcacgga taccctgcca ggtagaagcc
    18121 agtttgatca gaatacgatc gttgctaatc cctgcatcgt tgtagagttt gatcaggcgt
    18181 tttgctttcg caattgacgc ttcggtgtca taggaaagac gcgcatcaac ttcagttgag
    18241 atacggcccg gaaccagttt caggatttcc agaccaatat ttactgccag tttgtcggtc
    18301 gcgtccacga tctgctgcgc gcgatcgttg ctctgctgtt tcgcccaggc gacagcatca
    18361 tcaatcaact tacggtattc cggaatctgc gctgcgttaa gaatgagaga agggttggtt
    18421 gtggcatcct gcggttgata cagcttcatt gccgcgatgt ccccagtgtc ggccactacg
    18481 gtggtgtact gacgaaggga ggtcaatttg tccgtcatga tagtatttct ctttaaacag
    18541 cttgttaggg ggatgtaacc ggtctgccct gatgatatca cgacgcaacg tcagcgcaac
    18601 cgccgacaag acggttatcg ccaggcgaga ctgtttcgga tttctgacac tgcgttgata
    18661 tcgcccgcca tttttataca aaacctcatg tatgctacgc agaagttatc aagtacctcg
    18721 tagcgtatat acttcttaaa caattggtaa cgtttacaca ggaaagtcat cgcgaccggc
    18781 aataagaggg atatgcatgc cagatttttt ctcctttatt aacagcgtcc tttggggatc
    18841 ggtaatgatt tacctgctct tcggcgcagg ttgttggttc acttttcgca ccggatttgt
    18901 gcagtttcgc tacatccgcc agtttggcaa aagtcttaaa aatagcattc atccacagcc
    18961 aggcggttta acctcatttc agtcattgtg taccagtctt gcggcgcgcg tgggtagcgg
    19021 caacctggct ggcgttgcgc tggctattac caccggtgga ccaggtgccg tcttctggat
    19081 gtgggttgcc gcgtttatcg gcatggcgac ctcgtttgcc gaatgttccc ttgcacaact
    19141 ttataaagaa cgtgacgtta atgggcagtt tcgtggcgga ccggcatggt atatggcgcg
    19201 cgggctgggg atgcgctgga tgggcgttct gttcgccctc tttttgctca tcgcctacgg
    19261 cataattttc agcggagttc aggcgaacgc cgttgcccga gccctgagtt tttcttttga
    19321 ttttcccccg ctggtgacag gcattattct tgctgtcttt gctctgctgg caatcactcg
    19381 cggtcttcat ggcgtcgccc ggctcatgca ggggtttgtc ccgttgatgg cgataatctg
    19441 ggtactgacc agcctggtaa tatgcgtaat gaatatcggg caacttcccc acgtcatttg
    19501 gtctattttt gagagtgctt ttggctggca ggaagcggca ggcggcgcgg cgggatatac
    19561 cttaagccag gcgattacta acggttttca gcgcagtatg ttttccaatg aggcgggaat
    19621 ggggtcgacg ccaaacgcgg cagcggcggc tgcgtcctgg cctccgcatc ccgcagcgca
    19681 aggaattgtc cagatgattg gcatttttat cgacaccctg gtcatctgta cggcaagcgc
    19741 catgctgata ttactggcgg gtaacggcac aacctacatg ccgctggaag gtattcagct
    19801 tatccagaag gcgatgcggg tactcatggg ttcctggggt gctgagtttg ttaccctcgt
    19861 ggttattctg tttgccttca gctccatcgt tgccaactac atttatgccg aaaacaatct
    19921 cttcttctta cgcctgaaca accctaaagc gatctggtgt ctgcggatct gcaccttcgc
    19981 aacggtcatc ggcggcacct tgctaagtct tccgctgatg tggcaactgg cagatatcat
    20041 aatggcctgc atggctatta ccaatttgac cgccatatta ctgctctcgc ctgtggttca
    20101 taccattgcc agtgattatc tacgccagcg taaactcggc gtgcgcccgg tgtttgatcc
    20161 gttgcgttat ccggatatcg gtcgccagct ttctccggac gcgtgggatg atgtttcgca
    20221 ggagtaatca caactatcga tcaactcatt ctcttttttt gctaaagtcg gcataaattt
    20281 cctgcaagga ctggatatgc tgattcttat ttcacctgcg aaaacgctcg attaccaaag
    20341 cccgttgccc accacgcgct atacgctgcc ggagctgtta gacaattccc agcagttgat
    20401 ccatgaagcg cggaaactga cgcctccgca gattagcacg ctgatgcgca tcagcgacaa
    20461 actggcgggt atcaacgccg ctcgctttca tgactggcag ccagatttca cgccggagaa
    20521 tgcccgccag gcgattctgg cattcaaagg tgatgtctac accggcttgc aggccgaaac
    20581 cttcagcgaa gacgatttcg attttgccca acagcatttg cgaatgcttt ccggcttgta
    20641 tggcgtactg cgcccgctcg atttaatgca gccttatcgt ctggaaatgg ggatccgtct
    20701 tgagaatgcc cgagggaaag atctgtatca attctgggga gatatcatca ccaacaagct
    20761 gaacgaggcg ctcgcagcac aaggcgataa tgtggtgatt aatctggcgt cagatgagta
    20821 ttttaaatcg gtgaagccga agaaattgaa tgccgagatt atcaagccgg tgttcctcga
    20881 tgagaagaac ggcaaattta agatcatcag cttctacgct aagaaagcac gcggtctgat
    20941 gagtcgtttc attattgaaa atcggctgac caaaccagag caactgactg gttttaatag
    21001 cgaaggttac ttctttgatg aagcttcctc cagcaacggc gaactggtgt ttaaacgcta
    21061 cgagcagcgt taagtttgca gtcaatgccg gatggcaatc cggcattgat atttagcgat
    21121 gatgtttgtc aggccccgga cgatgatcgc cacgatgatc attgtgcttg ttatggcgcg
    21181 gcgatggcgg cggtccatat gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    21241 accagccgtg gtcgcgccag tgaccgccat cccagtaata gccacgatta tcacgatcgc
    21301 ctatctgtaa ttttactgac gggactaacg tcatttccgc agcctgcgca gccatgggag
    21361 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    21421 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac ggcagttgtt
    21481 attctccgca atcctaatct ctaagttacc gcatttattg agcatttctc ctttttccct
    21541 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
    21601 tgattgagat aatgaataga tattactgat gattcatcat caatttacgc aacgcagcaa
    21661 aatcggcggg cagattatgt gaaagcaagg gtaaatcggc acgttctgcc agctcttttg
    21721 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    21781 cggtgccgag gaacaagcca tattcgcctg gatgcaactg gtcacgcagt gcacgatacg
    21841 ctacggcagc gtgcggctcc gaagtgtagc ccagttcttt taactcacgc attgtctgtt
    21901 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    21961 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    22021 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
    22081 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca
    22141 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    22201 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
    22261 tgatgttaat cgagttagct gagtttagcc ccagcgccac tttcagttct tcatcatcaa
    22321 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcaa
    22381 tattgccgcc caatgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    22441 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gccgctccgg
    22501 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca
    22561 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
    22621 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaagaca aacgccgcgc
    22681 gtacgcgctc ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct
    22741 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
    22801 ccggcaggtc gtgcgggaaa aacagcccct gatttttgcc caacccctgg gttacggctt
    22861 gcgcaaagct gacctgctca ttgtgatctt tcagattgta gagtttcatt tagttttcca
    22921 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    22981 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacacagag
    23041 cgaacaaagt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg
    23101 cctgccgcgc ctgccggaag cctggcagta accgttcacg gtagggttca gcgataacat
    23161 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga ataaagcctg
    23221 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    23281 tggcttctgc cgtcgagact ttaatccccg gatacgccag cacccacagc cactcatcaa
    23341 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccga
    23401 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgccct tccagctcgc
    23461 ccatcaaagc cagcaaacgg gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca
    23521 tcagcgccgc gaccactgag caggcgctgg agcctaagcc cgagccgatc ggcatattct
    23581 tttccagggt catcgccact ggaatttgct tacccagctc ctggcaaaaa cgctcccaac
    23641 actgataaac gatattttcc cgaggttctg acggcagctt atcggcaaag cgtccgaggt
    23701 tattgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    23761 caacgggtgt caccgccgcc ccgagcacat cgaacccgac gctcatattg gcactggaag
    23821 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    23881 tcggcaaaga caccggcagc tgtaacgtca ttgcccgcac catatccgcg cagcaccaac
    23941 ggcagcggct gataatagtg gctatagaag gccagggcgt tttcgccgtt tttcactttg
    24001 aacagcggat cgttgccatc cacttcagca atcttcacgc ggcagacgcc atcttcatca
    24061 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac acgagcggca
    24121 aagagatcat cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
    24181 aactcagcgg gtagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    24241 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcgga
    24301 tccggttcgg tataacccat ttcccgcgcc aacgtggtcg cctcggagaa actcatgcct
    24361 tcgtctaact tgccgaagat ataagaaagc gaaccagaaa gaatgccgga gaacttcatc
    24421 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    24481 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcatgacg caacagatgg
    24541 tagtaatcca tcgacgaggt gttagccttt ttgttcggcg tgacgacgtg gaaaccttcg
    24601 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
    24661 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    24721 ttggcttgcg ccagttcttc ctgccagttt tccagattaa ggccatgtac attggtgagc
    24781 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcaaccag
    24841 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    24901 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    24961 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    25021 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
    25081 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
    25141 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
    25201 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    25261 atcagcacca cggaaatacg ggcgcgtgac atcgctgcaa agacgcgcgc cgccatgccg
    25321 accatccctt tcatccccgg accagaaacg ctgaacattg ccatgttatt cagattggaa
    25381 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
    25441 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatt
    25501 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
    25561 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
    25621 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
    25681 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
    25741 cctgccatca acaccatgtg atcagccgga atacgacttg ccgcaatacg gcgggtggac
    25801 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
    25861 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    25921 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    25981 ctaatgccat gcaggacatg ttttatttgg gcaaattcct ggtcgacgaa agttttcagt
    26041 tgcgccagcg ggaatcccgg ctgagcggcg gcgagtcccg tcagaagttc ggcaaaaata
    26101 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc
    26161 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
    26221 ctggcattgc tttccagaat atcggcaacc cgcagaaaac gttctgcatt tgccactgat
    26281 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    26341 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    26401 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    26461 tgtactctgt aatttttatc tgtctgtgcg ctatgcctat attggttaaa gtatttagtg
    26521 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    26581 agacactctt tttttaatcc acaccgagac atattgcccg ttgcagtcag aatgaaaagc
    26641 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    26701 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgt tgtaaccagt
    26761 cgaccagttt tatgtcatcc gccactgcca gagtcgtcag cagtgccatg gctcgttcgc
    26821 gtaaagcttg cagttgatgt ttgtctgccg ttgtgtcact tttcgccggt tgttgtatta
    26881 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    26941 ccgccaccat cggcacacca gtaagaacgt cagccaacgc taactcttcg ttagtcaacc
    27001 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    27061 acagcagcac cagttctact ggcgtagcgt agtaatgata tttcgcccga ctgcgcgcag
    27121 tggtggcaac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    27181 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    27241 cctgactatc gacaatccgc agctcgctaa accccatcgt tttcattgcc cgcgccgctg
    27301 ccccaatatt ttctgctctg gcgggtgcga ccagaataat cgttatacgc atattgccac
    27361 tcttcttgct caaataaccg cgaaccgggt gaccactgtc aacttattac gcggtgcgaa
    27421 tttacaaatt cttaacgtaa gtcgcagaaa aagcccttta cttagcttaa aaaaagctaa
    27481 actatttcct gactgtacta acggttgaat tgttaaaaaa tgctacatat ccttatgttt
    27541 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    27601 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    27661 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    27721 ttttattggg tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga
    27781 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    27841 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    27901 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    27961 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    28021 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    28081 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    28141 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    28201 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    28261 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgact
    28321 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    28381 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    28441 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    28501 ttctgtgaaa acccaggcaa aattcagtcc cgtgctgaac tgctgaagaa aatgaccggc
    28561 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    28621 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    28681 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    28741 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    28801 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtcgatatta cgggtcacaa
    28861 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    28921 cagagttgag cagtccccac atcacaccat ccagcaacaa cagcgcgagg gtaaacaaca
    28981 tgctgttgca ccaacctttc aataccgcct gcaaataaat accgttcatt atcgccccaa
    29041 tcagactggc gattatccat gccacggtaa aaccggtatg ttcagaaagc gccagcaaga
    29101 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    29161 gcgcggtgag cgtttcaaaa acaaagaacg ctataaaagt cagtgcaatc agcagaatgg
    29221 cgtacttagt cgcccggtca gttaattggt attgatcggc tggcgtcgtt actgcgacgc
    29281 taaacgccgg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    29341 tattagcaaa ccagctgctt tgccagtgcg cctgaaaacc tgactcgcta acttcccgtt
    29401 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    29461 cgctattacg cccgccaggc accacagaaa gatcgccggt accgcttaaa ttcagggcca
    29521 tattcagctt caggttctgc ttccgccagt ccccttcagg taaagggata tgcacgccct
    29581 gcccgccttg ctctaagccg gtgccgggtt caatggtcag cgccgttccg ttaacttcag
    29641 gtgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aatggcttgc
    29701 ctaaggtgat atttggcgcg ttgagttcgc taagacgcga aacatcgaaa tcggctttta
    29761 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    29821 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
    29881 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgatga
    29941 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    30001 tacggtaatc agcacgttcg acaatcacct gccgaatcag cattatcgga atcaacaaca
    30061 gcaatactgc accaaacagg gtggtcattt tccagaacag gggggatttc aacatagtct
    30121 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    30181 agtgacggtg aagtcgaagc gaagccagca cgccaccttc ctgcacattg cgcagcgtga
    30241 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    30301 tttgcccatt tgcacgaggc aaagagtaaa agcgttcaaa aatacgtgaa agcgcgtagt
    30361 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctgc tccacttcgg
    30421 cgcttagcgt gatgcaaccg ctctcggggg taaaatcgat ggcgttatcc agtaaattcc
    30481 ccagagcctg ctccagtaac gccggttcag cagcaacgtt aacctcggtg ggcgtaacat
    30541 gtagagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcggaata
    30601 atgccgccac atcaacagca gttagaacga cttcctgacg attctccagt cttgcctggc
    30661 gtagtaacgt ttctaccaat gcctgcatac gcgcattttg cgtcagaatg ttgtcagtaa
    30721 aacgagccac cacttccggc ggcggacctt cgcgtaaaat ttccgccgct ccacgaatcg
    30781 ccgccagtgg gctttttagc tcatgagtta atgcgtaaac atactgctca atatagtttt
    30841 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    30901 taccgagatc ggggagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    30961 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    31021 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    31081 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg tccataatcg
    31141 gcgcggcaac atacatcacc gaactttcgg gatcggtagg attttgcaac gtgctgcgcg
    31201 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    31261 cggctttatt tgccgaatcg aacaatactt tgccctgcgc atcggtcata tagacatgat
    31321 attcattgcg cactttgtta atgccaccga tattggcgcg aaacgggcga tgctgtagct
    31381 gattaaacgc ctgcgccagt tgcccatgcg ttgtgtcccc agagagcaaa tcgggacgcg
    31441 ccagctccgc cagcaacgtt gcggtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    31501 ccggtttaac ttctttgaca aaaatggcca gtacgaacca agctgccacc gccaccagta
    31561 aaaaatagcc cagcaacaac cgcatgccga tacgcattac aggcccctca ggctatatcc
    31621 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    31681 cagcgtttta atgtgggtat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    31741 gctatccatc agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    31801 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    31861 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    31921 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    31981 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    32041 ggtcaggaac agtacaggta acgccggatg gagcgtcagt aactggcggc acaattcaaa
    32101 gccactgata tcaggcaaac caacgtcgag aatcatgaca tcgggaacct gctggcgagc
    32161 tttatccagc accggcaagc ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    32221 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    32281 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    32341 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    32401 agcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    32461 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    32521 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    32581 accaccggtt ttcgcccgac tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    32641 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    32701 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    32761 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    32821 aataacatat tgattaagaa caaaatctca atacttttat ttccgtcaag ccctaaaaca
    32881 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    32941 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    33001 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    33061 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    33121 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    33181 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    33241 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    33301 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cactatgcca
    33361 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    33421 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    33481 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    33541 agtcaggata aagacgacga acaagaagta ttctatacca ccgcgttagc ccaggatcag
    33601 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    33661 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacgggacg
    33721 tgcatgccaa tgctcaacct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    33781 gcagaagatg ccaaagcggg agatcgccca attaatctgc gctgtgaact actgattccg
    33841 atccgtcgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    33901 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    33961 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    34021 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    34081 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    34141 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    34201 tcttcggtga gtgaatcgat atttcttgtt tccagtacac ccatatttaa ttcacgcagg
    34261 cgagaatcaa agatgatatc acagccgcag gcctgggcga tgatttccgc cgtgcgccgg
    34321 gtccgtccta aatcgctact gatgatatgc gtaatgccaa gctctttggc acgggttgcc
    34381 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    34441 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    34501 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    34561 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    34621 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    34681 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    34741 tacatccaga ggctgatttt tgggtggcga ttgaagcagg cattgatggc gacagcacct
    34801 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    34861 cgttacctgc ggtgattctg gaaaaagtgc gtgaaggcga ggcgctcggt cccgtgatgt
    34921 cacgttatac cggtattgat gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg
    34981 ccgggaaact tacccgcgcc agcgtctatc accaggcggt gatcctggcg ctgagtccgt
    35041 ttcataatgc ggtgtatcag tgatctttca gcaacacctc ttccagccac tggcgcagct
    35101 ccacgggcgc ggctttcagg ctgttagatc cacgcgtaat cgtcgcgatg cccgcgccga
    35161 gttcattttt taactcacgc tggctcattt cgccgcgcaa cagctcttcg acaatacgca
    35221 cgcgagtccc caacgcttcg cgctcatctg gcgtcagcat caggtttaac aacggtaaat
    35281 ggagatcgtt ttggtaggca ttcttaagca ggtcgacaaa acgtaaccac tcctggtgac
    35341 gctgttctgc catcgctgct gaatagggtg attgttgggc cataatatgt cgccattgtt
    35401 agcgggggaa gcaaaatgcc tcccccggtt gtactcgcta aagagtacga tagcatatca
    35461 taaacgtgcg gatcagtaac gacgtcccca ttccgtggcg ctcatcaacg tcggtttatc
    35521 ccccatgaaa tagcggtagt aagcgtcata agccagcacg ttcttcacat aaccgcgcgt
    35581 ctcggagaac ggaatactct cgacaaatgc cacagcgtcg atacgcccgg cgctgttgcc
    35641 aagccaggtt cgcacccgtc caggtccggc gttataagct gctgaggaga aaatacggtt
    35701 attgccaaac tgctgataaa catattgcag gtaactggtg ccaatgttga tgttcgtttc
    35761 cggatccagc aattgcccag ggctgctata accaggaata gagaacatct tcaccgtatg
    35821 ggtcgccgtg cccggcataa tctgcatcag gccgctggcc cctaccggtg atttcacttt
    35881 cggattccag gcgctctcct gacgggcaat cgccatcgca tagctttgcg ggatctcctt
    35941 cccactggtg taacgtttga aaagatcgtt gtaggccagc gggaatctct cttccagatg
    36001 atcccaaagc ttcccggcga tcgttgcctg aacgctaagt tcccaccatt ggttattgaa
    36061 agcataccga gccagttgcg cctgctctgt ttttgacttg ctcttcacca gattggccca
    36121 ctcgctacgc gcggtattat cgagattcca gtacatcaac tcgcgcacgc gcgccatctc
    36181 cggcccctga gtcagggcgc tgtcaacatt ctgcggcgct ttatcaatct tcagctcata
    36241 ctcttcaccg atgcgttgtg ctgcaaccat cgggtagaaa ccacgctgtt gcatgagttg
    36301 atgcaaaatc tcttttgctt cagcttcacg tccgcgttcc agcagtaaat ccgcctgcca
    36361 gtaacgccat tcatctttct ctttcgcctc catcggcaaa cgcgccagcc aggtattcag
    36421 gccgcgacga tcgccggtgc caagcgccat tcgcacgcgc cgttcaataa gcgaagtaga
    36481 ttgcgaacgc ataatggcgt catcgcgcca tttcgcctgc tcatcggtga catcattgcc
    36541 catcaaacgc caggcgacga tatcgcgcag ctcctgaatc tgatcttcat taagctgctg
    36601 cgcctgggca agcgacggga tcatcagccg tgcattttca gcatcctgcc gcgccacact
    36661 ggcaaacgcc accgccgcca tttgacgggt aaaatcggtc gctccggttg tacgcgcgaa
    36721 ggtcagtacc gtattagggt tgttcgccag tgaaatgatt gccgaggcga tagtctggta
    36781 atcggcaggc atctgccctg ccagcactgt tacgagtcca gtgttacccg ctttcatcgc
    36841 cagacggata cgctctaaat acgccagcgg atcttgttta cctgacgcac gccagacgct
    36901 aaataacttg tcacaggcgt taggctggct cttgccggtt agccacagct ctttcgcccc
    36961 ttgccaggct tcttcactct gcccggtgtt ccatttcgca tagtagtaat tacattgcgc
    37021 ttcggtagtt ccgggctttt ccgggctaaa ggctaacaag ccacgccagt cttcacgccg
    37081 cgccagttca ttgacgaaac gagattgcag cgtgcgagcg ggaggaagcg tggggttagc
    37141 gcgaacaaag ttagtgaccg tcaccgccgg ttgattcatc aggtcatcgg tgatctgacg
    37201 gtattccagg tagggataaa gcggataatc tttcagtcca ggcatcattt gttcgaccac
    37261 atccatttgt cgattatccc aggcctgctt gatttgggcg tagcggctac gctgctcatc
    37321 cagtgagtcg gctcgcgcca cgctgctgac cgtcagcaga cagacaccgg cagccaacag
    37381 ccgccaggta acttgtttgg ctttttccac aagcgcatcc tctaagtgta aatacatcaa
    37441 tgcagcgtca tgccgcgtaa tagatttatg ctaaccagtc attgccgttt acgccacgtt
    37501 acggacactt ttttactttt atactctaaa taattcgagt tgcaggaagg cgataagtga
    37561 gtgagtcccc aggagcttac agaagtaagt gactggggtg agcgagcgta agcgcagcac
    37621 atgcaacttg aagtatgacg agtatactgc gagggtgatc gaccggctgg gattagataa
    37681 ggaaacaggc tacacttcgc ctttgaaaac tcatcatcac gataaacata gaggcgaagt
    37741 ccaacgtggc tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac
    37801 gtcatatttt gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg
    37861 gtctgaacgg cgcgggtaag tccaccctgc tgcgcattat ggcgggcatt gataaagaca
    37921 tcgaaggcga agcgcgtccg caaccggata tcaagattgg ttatctgccg caggaaccgc
    37981 agttgaaccc ggaacacacc gtgcgtgagt ccattgaaga agcggtttct gaagtggtta
    38041 acgccctgaa acgcctggat gaagtgtatg cactgtacgc cgatccggat gccgattttg
    38101 acaaactggc cgctgaacag ggccgtctgg aagagatcat tcaggctcac gacggtcaca
    38161 acctgaacgt tcagctggag cgtgcggcgg atgcgctgcg tctgccggat tgggacgcga
    38221 aaatcgctaa cctctccggt ggtgagcgtc gtcgcgtggc gttgtgccgc ctgctgctgg
    38281 aaaaaccaga catgctgctg ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg
    38341 cctggctgga acgcttcctg cacgacttcg aaggcaccgt tgtggcgatt acccacgacc
    38401 gttacttcct cgataacgtt gcgggctgga tcctcgaact ggaccgcggt gaaggtattc
    38461 cgtgggaagg taactactcc tcctggctgg agcagaaaga tcagcgtctg gcgcaggaag
    38521 cttcacaaga agctgcgcgt cgtaagtcga ttgagaaaga gctggagtgg gtacgtcaag
    38581 gtactaaagg ccgtcagtcg aaaggtaaag cacgtctggc gcgctttgaa gaactgaaca
    38641 gcaccgaata tcagaaacgt aacgaaacca acgaactgtt tattccacct ggaccgcgtc
    38701 tgggcgacaa agtgctggaa gtcagcaatc tgcgtaaatc ctatggcgat cgtctgctga
    38761 ttgactccct gagtttctcg atcccgaaag gggcgatcgt cgggatcatc ggtccgaacg
    38821 gtgcgggtaa atcgacgctg ttccgtatga tctctggtca ggaacagccg gacagcggca
    38881 ccatcacttt gggtgaaacg gtgaaactgg catcggttga tcagttccgt gactcaatgg
    38941 ataacagcaa aaccgtttgg gaagaagttt ccggcgggct ggatatcatg aaaatcggca
    39001 acaccgagat gccaagccgc gcctacgttg gccgctttaa ctttaaaggg gttgatcagg
    39061 gtaaacgcgt tggcgaactc tccggcggtg agcgcggtcg tctgcatctg gcgaagttgc
    39121 tgcaagttgg cggcaacatg ctgctgctcg acgaaccaac caacgacctg gatatcgaaa
    39181 ccctgcgcgc gctggaaaac gccctgctgg agttcccggg ctgcgcgatg gttatctcgc
    39241 acgaccgttg gttcctcgac cgtatcgcca cgcacatcct ggattaccag gatgagggta
    39301 aagttgagtt cttcgaaggt aactttaccg agtacgaaga gtacaagaaa cgcacgctgg
    39361 gcgcagacgc gctggagccg aagcgtatca agtacaagcg tattgcgaag taatgcgtaa
    39421 aatgccggat gcggcgcgcc agcatcgcat ccggtattag ttaaatgatt aatgggaggc
    39481 aagttcccgg aaaaagtcag gattttcttt aattgtggca agtacttttg cagccaatcc
    39541 cgtaggattt cttcgcttcg attcccagct tttaatggta tccacactgg ttcccagagc
    39601 ttttgccatc tcactttgcg aaacgttgag ttgctcacga atagctttaa catccgcaat
    39661 ttcgtagcgg gtcacccgtg caggttcttt taaaccttgt ttaatctcga cagcctcttc
    39721 cagagaggtt ttcaactcat caaaaaaact cattttatat ctcgcctttg agcagtttcg
    39781 tcaacttctt tagttgagct ttttccgtat ctgtcagact atctttggca tttttcggat
    39841 aggccataat caaataaata atctcttccg tcgccagaaa gtaaattatt ctgacactgc
    39901 cactttttcc ctgtgaaccc gcagccattc ggactttacg caatccacca gtctgctgaa
    39961 tgagatcacc tttatcggga gttccgatga gttctttttg taattccttt aattcatcat
    40021 cggttgcaat ctgcttgatc tgacgcgtaa acattggcgt ctctataaat attatcgcct
    40081 tacccattac ccctccctgg aaggtacatt gtacaccaaa atggagtact aagtacactt
    40141 ttgaatcagt aatcccactc aacatcttat aaagatgatt atctttgctc ccccatcatc
    40201 tcccgcacca gctccacgca gcgcaggaaa cggctgtcat aatcgtcttc ttcaacccgc
    40261 acaaattcga tattgttctc ttccagcatc tccaccagca agttctggaa ctctttgcga
    40321 tccaccgaac tgccgaggct gcgtaaacca tccgccaccc aaggggtgtt gttctccagc
    40381 aagataacca gatcaaaacg gtattcatcg atcagcgcct gcacgaacgg atgctcccgc
    40441 ccttcgtact ttttgcagaa cgcctgggta gtgacaaaat cagtgtcgat aaacgccact
    40501 ttattggcat atttcactgc aaaatcaatg tattgtgcgt gtcccagcgc gattttatcg
    40561 tagtcggaat actgcaatgc gatctcatca ccgccgaggt gtgaaaagac ataatcgcga
    40621 ccatattccc acgcactggt ggtattgaag atattggcaa gtttgtttac cagggtggat
    40681 ttaccgctcg actcgccgcc aaggatcgcc acggtacgca caaaaaacgg cttcacttca
    40741 gtggggatat attcccagta gcggaacggg ttttcgcgga tctgcgcacc gctgatactc
    40801 ataaaggtgc gtttcggatc gaccagcacc gtttcgatcc ccagatgttc catatactgt
    40861 ggcgcatcgg cttcttccga ggtgtagatc aggtccggct ggatcccctt ttcagccata
    40921 aactttttga tgccgttgct ccatacatcc cagccgtgcg gatatggctc catgccctct
    40981 tcattgaaag catgaatacg gatatttttc tgatatttaa aggtttgcaa taaccagcgc
    41041 agacgatccg gcaccgtggg ctgctgcgac atggcgctgt cttcgaacaa cgcgcggtcg
    41101 cgggtatcgt caaagcccat aatgatatgc agctcgtcaa cctggctaca ggcgcgctgg
    41161 ataaggtaga tatgtccggt atgcagcggg tagaacttac cgaatacgac accaatggtt
    41221 ttcttctgtc ggggaaattc aagccccaaa aaacggtgca acgcctccag cttttgcgcg
    41281 ctggggcttt taattttggc attcagtaac tggcttaaat accctttggt cataccgctg
    41341 gcatcagcga cctgctgtag cgtgcagccc tgttgcttga tggcagtttt caggtaatca
    41401 aatgacgaca tatctccctc cgtatctctc attataagtc gtcgaacacg ctaagcgcgt
    41461 cggagagttt tttaacgcca aaaatctgca tcccttccgg cgcttttttc ggcacgttag
    41521 ccgccggaac aatcgcccgg cgaaaaccgt gtttcgccgc ttcagaaatt cgttcctggc
    41581 cgctgggcac cgggcggatc tcccctgcca gcccgacttc accaaacacc accagatcct
    41641 gcggcagcgg tctgtcgcgc aggctggaaa ccatcgccag cagtaacgct aagtcggcgc
    41701 tggtttccgt taccttcacg ccgccgacca cgttaacaaa tacatcctga tcggccattt
    41761 gcaacccacc gtgacggtgc agcaccgcca gcaggattgc cagacggttt tgctccagcc
    41821 ctactgcgac gcggcgtggg ttcgccatca tcgagtgatc gaccagcgcc tggatctcca
    41881 ccaacagtgg acgcgttcct tcccacacta ccatcactga gctaccggag gtcacttcat
    41941 caccgcggct taaaaagatg gcggaagggt tgctaacttc acgcagcccc tgttcggtca
    42001 tcgcgaagac gcccagctca ttcaccgcgc cgaagcggtt tttatggctg cgtaaggtgc
    42061 gaaaacgaga gtcggcatcg ccatccagaa gcaccgaaca gtcgatacag tgttccagca
    42121 ctttagggcc agccagcgag ccatctttgg ttacgtgccc aaccatgaca atcgccacgc
    42181 cgcgcgtttt ggcgaagcgc gtcagataag ccgccgtttc acgcacctgc gccacgctgc
    42241 caggcgatga ctgaacatcc gccatatgca tcacctggat ggagtcaatt accatcagct
    42301 tcggttgctc ttcttcggca atcaggcaga tctgctcgat gctggtttcc gacagcatat
    42361 taagattgtc agtcggcagg ccaaggcgat gagcgcgcat tgccacctgt tgcagcgact
    42421 cttcgccagt gacatacagc gttttcatct gctgcgccag tttgcacagc gtttgcagca
    42481 acagcgtgga tttccccgca cctgggttac cgccaatcag aatggcgctt cccggcacca
    42541 ccccgccgcc taatacgcgg tcgaactctt taaatccggt ggaaaaacgc ggcagctcct
    42601 caaggctgat gtcggaaagt ttctggactt ttgccactcc ggcgctaccg gcatagccac
    42661 tgagacgctc gttacgcgcc accgttggcg acgcagcaag acgcacctcg gtgatggtgt
    42721 tccaggcatg acaggcgctg cactgcccct gccagcgcgg ataatcggcc ccgcattcat
    42781 tacaaacaaa ggcgcgtttt ggagcttttg ccacggtgta cctcgttaat gctgtgccgc
    42841 ccgcaggatg acgggcaggc aattacttct gattcaggct gccagagagg atgcagaata
    42901 cccccatcag gtcagcgtga cggatggtga cttccgtctt ttcattcact tttggcttgg
    42961 catggtaggc aatccccagc cctgccgctt tgatcatcgg caggtcattg gctccatcgc
    43021 caatcgccac ggtctgcgcc agcgggattt catactcctg cgccaggcgg gtcagggttt
    43081 tcgctttata ttgcgcgtca acgatgtcgc cgatcacatt gccggtaaat ttaccgtcca
    43141 tgatctccag ttcattggct accactgctg tcaggcgcag cttgtcgcgc aggtattcag
    43201 caaagaaagt aaagccgccg gaggcaatcg ccactttcca gcccagcgtt tccagcttga
    43261 gtaccagctg cgttaagcct ggcatcagcg gcagattttc acgcacctgt tgcagaatat
    43321 tggcgtcagc gcctttcagc gtcgccacgc ggctgcgcag gctggcggta aaatcgagtt
    43381 cgccgcgcat cgcccgttcg gttacctccg ccaccctctc gcccgttccg gccagtttgg
    43441 caatttcatc aatacattca atctggatgg cggtggaatc catgtccatc accagcaaac
    43501 ccggcgtgcg caggtgcggg attttcccca gcggcgcgac atccagctgc gcttcgtggg
    43561 ccaggcgcgt agcccgcgcg gtgagtgaac ctgccagacg aatcacctga taatcttcca
    43621 cgcaccaggc ggcaacaatc accatcgccg cgcccagttt gctctggtat tgggtcagac
    43681 gttgtttatc cagcccacga ccatacagca gccagccgct acgacctgcg tggtaatcca
    43741 gtggcatcac ttcatcacca cttaatgaaa gaggcagacc cggccataaa gagacatctt
    43801 caggcaggtc gcaccaggta atgttaggca ttaaggctcc tgtaaaatcg ttcgaagcag
    43861 ggaaaataac gcatgaggct accttgtatc cattgcttct ggcaacatta agtctcaaat
    43921 tttcaaaggg tggaagatgg ctcgcacaaa actgaaattc cggctgcatc gggcagtgat
    43981 tgtcctgttc tgtcttgcct tgttagtggc gctgatgcag ggagcgtcat ggtttagtca
    44041 aaaccaccag cgacagcgta atccacagct ggaagaactg gcccgcaccc tggcgcgtca
    44101 ggtgacgctg aacgttgcac cgctgatgcg taccgactca ccggatgaaa aacgcattca
    44161 ggcgatcctt gatcagttaa cggatgaaag ccgtatcctc gacgcgggcg tgtatgacga
    44221 acaaggcgat cttatcgccc gttctggcga aagcgtcgac gtgcgcgacc ggttggcgct
    44281 cgacggcaaa aaagcgggcg gctattttaa ccagcagatt gtcgaaccaa ttgccgggaa
    44341 aaacggaccg ctcggctatc tgcgccttac gctcgacacc catacgctcg ccaccgaagc
    44401 ccaacaggtg gataacacca ctaacatttt acgcctgatg ttgctgctct cactggcaat
    44461 cggcgtagtg ctgacccgca cgctgctaca gggtaaacgc acccgctggc agcaatcgcc
    44521 cttcctgtta accgccagta aaccggtgcc ggaagaggaa gaaagcgaga aaaaagagtg
    44581 acccattact acaagaaagg aaatcgttat gtccacatta cgcctgctca tctctgactc
    44641 ttacgacccg tggtttaacc tggcggtaga ggagtgtatt tttcgccaga tgccggctac
    44701 gcagcgcgtt ctgtttctct ggcgcaatgc cgacacggta gtgattggtc gcgcgcagaa
    44761 cccgtggaaa gagtgtaata cccggcggat ggaagaagat aacgtccgcc tggcgcggcg
    44821 cagtagcggt ggcggcgcag tgttccacga tctcggcaat acctgtttta cctttatggc
    44881 tggcaagccg gagtacgata aaaccatctc cacgtcgatt gtgctcaatg cgctgaacgc
    44941 gctcggcgtc agcgccgaag cttccgggcg taacgatctg gtggtgaaaa ccgccgaagg
    45001 cgatcgcaaa gtctcaggct cggcctatcg cgaaaccaaa gatcgtggct tccaccacgg
    45061 cactttgctg ctcaattccg accttagccg cctggcaaac tatctcaatc cggataaaaa
    45121 gaaactggcg gcgaaaggca ttacctcagt gcgttcccgc gtgaccaatc tgaccgaatt
    45181 gctaccgggg atcacccatg agcaggtttg cgaggccata accgaggcct ttttcgccca
    45241 ttatggcgag cgcgtggaag cggaaatcat ctccccggac aaaacgccag acttgccaaa
    45301 cttcgccgaa acctttgccc gccagagtag ctgggaatgg aacttcggtc aggctccggc
    45361 attctcgcat ctgctggatg aacgctttac ctggggcggc gtggaactgc atttcgacgt
    45421 tgaaaaaggc catatcaccc gcgcccaggt gtttaccgac agtctcaacc ccgcgccgct
    45481 ggaagccctc gccgggcgac tgcaaggctg tctgtaccgc gcggatatgc tgcaacagga
    45541 gtgcgaagcg ctgctggttg acttcccgga acaggaaaaa gagctacggg agttgtcagc
    45601 gtggatggca ggggcggtaa ggtaactcgc tgattaaaat atccagggaa ggaaatatga
    45661 tcgatctgga aaaccaggaa cgggagatta tcaatttaat gttcagccag ggtatatcct
    45721 ggctaaccgc cgtgcgtatc aggcataaac tatcacttgc tgaagtaagt aaaatgttag
    45781 gtatctccat taattctcta aaacagatcg aaaagacgga acggcttagt tcaaacatca
    45841 aaagcaaaat ggcaggaatt tatggttgtc caccagagtt gctgatatgc ccttcctgga
    45901 tgacggctga acataaatga atagattcgt gaatagatac ttgaatggat tttattttct
    45961 atccatatga aaaataatag taaatctata aatcagttgc tattgatgaa taaatactaa
    46021 aaagccggag cagtctccca ctccggcttc acacgacaat cgcctttcag caaaacacaa
    46081 ttactcttta tcgcccagca gaacggattc cagcgcgatt ttgatcatgt cgttgaaggt
    46141 ggtctgacgc tcagcggcag tggtctgctc gtgagtgcgg atgtggtcag acacggtgca
    46201 gatggtcagg gctttcgcgc caaattctgc agcgacgccg tagataccag ccgcttccat
    46261 ttccacgccg aggatgccgt atttttccat cacgtcgaac atttcgccgt ccggagagta
    46321 gaacaggtca gcggagaaca ggttaccaac gcgagcatca acgcccagtg cttttgccgc
    46381 gtctaccgcg ttacgcacca tgtcgaagtc agcgatagcg gcaaagtcat ggtctttaaa
    46441 acggatgcgg ttaactttgg aatcggtgca ggcacccata ccgataacga cgtcacgcag
    46501 ttttacgtgc ggcagaacag caccacagga acctacgcgg ataattttct tcacgccgaa
    46561 atcggtgatc agttctttgg tgtagatgga gcaggacggg atacccatac cgtgacccat
    46621 tacggaaatt ttgcggcctt tataagtacc ggtgaagccc agcataccgc gaacgttgtt
    46681 cacttcacgg gcatcttcaa ggaaagtttc agcaatatac ttcgcacgca gcgggtcgcc
    46741 tggcatcaaa actacgtcag caaaatcgcc catttctgca ttaatgtgtg gagtagccat
    46801 cgttttatcc tttttgtgac atattaaagg cggagtcact gctccgccca aatccatcag
    46861 aacatggctt tgccatattc catatcagaa gtaccaaaat attttgccag agtctggccg
    46921 atatccgcga aggtttcacg atgccccagc gaacctggtt tcactttcgg gccgtaaacc
    46981 agtaccggaa tgtgttcacg cgtgtggtca gtaccggtcc aggtcgggtc acaaccgtgg
    47041 tcagcagtga gaatcagaat atcgtcatcg cgcagcaaag acatcagctc cggcagacgg
    47101 cggtcgaaca gttccagacc cgcagcataa ccggcgacgt cgcgacggtg gccccaggaa
    47161 gagtcgaagt caacgaagtt ggtgaagacg atggtgttat cacccgcttc tttcatctct
    47221 ttgatggtgg cgtcaaacag cgcgtccagg ccggtcgctt tcactttttt ggtgataccg
    47281 cagttggcat agatgtctgc aattttaccg accgaaacca cctggccgtg tttttcatca
    47341 accagtttct gcagcacggt cggtgctggc ggctcaacag ccaggtcgtg acggttaccg
    47401 gtacgctgga agttaccggc tttgtcgccg ataaacggac gagcgataac acgaccaatg
    47461 ttgtagccgc cgttggtcag ctcttcacgg gcgatttcgc acagttcgta gagtttatcc
    47521 agaccaaagg tttcttcgtg gcaagcaatc tggaacacgg agtcagcgga ggtatagaaa
    47581 atcggcttgc cggttttcat gtgctcttcg cccagttgat ccagaatgac cgtaccggaa
    47641 gagtggcagt taccgaggta acccggcaga ttagcgcgtt caaccagttt atccagcagc
    47701 tcttgcggga agctgttttc gtgatcggag aaatatcccc actcaaacag aaccgggaca
    47761 ccggcgattt cccagtgacc agacggggtg tctttaccgg atgacatttc gtgcgcccat
    47821 gcgtacgcgc cgataacttc agcgttgcca tccattcccg ccggaatgaa accggtagaa
    47881 ccttcgtgtg ctttcgccag cccaagacgg gtcagatttg gcagattgag cgggccttta
    47941 cgaccgtggt cagcttcgcc tttggcacaa gcttctgcga tatgacccag ggtgtcagcc
    48001 ccgacgtcac caaagcgttc tgcatcttct gtagcgccga tgccgaatga gtccagcacc
    48061 ataataaatg cacgtttcat atgttctccg tactcagtgc ttcaaattta aatgatcaga
    48121 tcagtatacc gttattcgct gatacggcga tagacagttg gtgtgctttc cggtgcttta
    48181 tcggcaagtt taattgccgc tttcaccgct ttcgccgctt cctgccagct gttttcgtct
    48241 ttcgcgtgga taactgccag cggacgctga ccgtctacct ggtcgcccag acgcgccata
    48301 tcagtaaagc cgacgctgta atcgatggtg tcagatgcct gacggcgtcc gccgcccatt
    48361 gcaaccactg ccatccccag tgcgcgggta tccatttcac tgacaaaccc ttcggtatca
    48421 gcatagactg ctttcgtcag catcgctgtc ggcagatact tcgcgtagtt ctcaacgaag
    48481 tcggtcgggc ctttttgtgc cgcgaccata cgaccaaaga cttctgccgc tttaccgttg
    48541 tccagcaccg cctgcaattt cgcgcgcgct tcggcgtcat ttttcgccag tttgccggag
    48601 atcagcatct ccacgcacag tgccatcgtg acatcaaaca gacgcgggtt acgatattca
    48661 cccgtcagga actgcaccgc ttcacgaact tcaaccgcgt tacctgcact ggaggccagt
    48721 acctgattca tatcggtgag cagcgcggtg gtgcgcacgc cagcgccgtt agccacgcca
    48781 acaatcgctt cggcaagggc ttcagagagt tcgtaggtcg gcataaacgc gccgctacct
    48841 actttcacgt ccatcaccag cgcgtccaga ccttccgcca gtttcttcgc cagaatagag
    48901 gcagtgatca gcgggatgga gtccacggtt gcggtaatat cacgggtcgc gtagaaacgt
    48961 ttatccgccg gagccagtga gctggtctgg ccgataatcg ccacgccgac gtctttaata
    49021 atttcgcgga aacggttgtc atccgggaaa atgtcgaagc cagggatgga ttccagtttg
    49081 tcgagcgtac cgccagtatg accgaggccg cgaccggaga tcatcggaat atagccgccg
    49141 caggctgcga ccatcggccc caacatcagc gaagtgacat cgccgacgcc gccggtcgag
    49201 tgtttatcaa caatcgggcc attcagatgc aggcttttcc aatcgagaac ggttcctgaa
    49261 tctcgcatag ccatggtcag cgagacacgc tcaggcattg tcatatcgtg gaagaaaatg
    49321 gtcatcgcga gggcggcaat ctgcccttcg gagatagtgt tgtcgcgaat accgttgata
    49381 aagaaacgaa tttcttcatc gctcagcgca tgaccatcac gttttttacg aataatttct
    49441 tgtgcgagaa acaaggtaac ctcccgtgga aaagagacga ggtaatcggg gaaccggatg
    49501 agcatcgctg catcatccgg caaatcaagc cttagtagct gctggcgctc ttaccgtcgc
    49561 cgtggcccag agctttcagc aggcttgcca gcaggctgga agcaccaaag cggtagtgac
    49621 gcgcatcagc ccagtcagca ccgaacagtt catcggcgat ggcgagatat ttctgcgcat
    49681 cttccgcagt acgcacgccg cccgccggtt tgaaaccaac ggttttttct acgcccatat
    49741 cacggatcac ttccatcatg atgcgcgcgc tttccggcgt cgcgttcaca gccactttac
    49801 cggtagaggt tttgatgaag tccgcacccg ctttgatgga gatttcagac gctttacgga
    49861 tcagcgcttc gtcttttagt tcgccggttt cgatgatcac tttcagcagt acattcgctg
    49921 ccgcgcaagc ctctttacag gctttcacca ggtcaaaacc aacctgctcg ttacccgcca
    49981 tcagcgcgcg gtacgggaac acaacgtcaa cttcatcagc accgtaggcg attgccgcac
    50041 gggtttctgc cagcgcgatt tcgatgtcgt cgttaccgtg tgggaagttg gttaccgtag
    50101 cgatacggat ttccggggta ccctgctctt tcagcgtttt gcgagcaatt gggataaagc
    50161 gaggatagat acagatagcg gcggtattgc cgaccggagt tttggcctga tgacacagag
    50221 caattacttt ctcgtcggtg tcgtcgtcat tcagggtggt caggtccatc aatttcagtg
    50281 cacgcaggct gcttgctttc agatcagtca tttcattctc ctggcttgtc gccaataaaa
    50341 ttcaccttgc gagtttgtta gtattctaac atctactccg caacacactt cgatatacat
    50401 cacaattaag gaaatctact tacaagtttg catcactgta atgcgatctg gttcaaataa
    50461 ttcactttca aatgaatgcg tcagtggtgg caaacgcatc aggatcaaag tgaacatcac
    50521 gaaacttctt acaatggcgc atcaccttcg gcataaaagg aaaagaagat gcccacttct
    50581 catgaaaatg cactgcaaca acgttgccag caaattgtca ccagcccggt acttagcccg
    50641 gagcagaagc gccattttct ggcactggaa gcagaaaaca atctgcctta cccacagctt
    50701 cctgccgaag cccgccgcgc gctggatgaa ggtgtaatct gcgatatgtt tgaaggtcat
    50761 gcgccgtaca aaccgcgcta tgtcttaccc gattacgccc gttttctggc gaacggttcc
    50821 gaatggctgg agctggaagg cgcgaaagat cttgatgatg cactctctct gctgaccatt
    50881 ctttaccacc acgtaccatc ggtcacatcg atgccggtct acctggggca actggatgcg
    50941 ttgttgcaac cgtatgttag aattctaaca caagacgaga tcgatattcg aataaaacgt
    51001 ttctggcgtt acctcgacag aaccctgcca gacgccttta tgcacgccaa tatcggcccg
    51061 tctgattcgc ccattacccg cgcgatctta cgtgcagacg cagagttgaa gcaggtttca
    51121 ccgaacctga cctttatcta cgatcctgaa atcacccctg atgacctact gctggaagtg
    51181 gcgaagaaca tctgtgaatg tagcaaaccg cacatcgcca acggtccggt gcatgataaa
    51241 attttcacaa aagggggcta cgggattgtg agctgttaca actcactgcc gctggcgggt
    51301 ggtggcagca cgctggtacg ccttaacctg aaagccattg ccgagcgcag tgaatcgctg
    51361 gatgacttct ttacacgcac tctaccgcac tactgccagc agcagatcgc catcatcgat
    51421 gcgcggtgtg aattcctcta tcaacaatca cacttctttg agaatagctt cctggtgaaa
    51481 gaagggctga ttaaccctga acgttttgtg ccaatgtttg gcatgtacgg attggcggaa
    51541 gcagttaact tgctgtgtga gaaagaaggg attgccgcgc gctacggtaa agaagccgcc
    51601 gcaaatgaag tgggttatcg catcagcgca caactggcgg agtttgtcgc caataccccc
    51661 gtgaaatatg gctggcaaaa acgcgccatg ctacacgcac agtcggggat cagttccgat
    51721 atcggcacca cgcctggcgc acgcttgccg tatggtgatg agccagatcc gattacccat
    51781 ctgcaaactg tcgccccgca tcatgcttat tattattccg gcatcagcga cattctgacg
    51841 ctcgacgaaa ccatcaaacg taatccgcag gcgctggtac agctttgcct cggtgccttt
    51901 aaagccggaa tgcgtgaatt taccgccaat gtcagcggta acgatctggt tcgcgttacc
    51961 ggttatatgg tgcgtttgtc ggatttagaa aaatatcgcg ccgaaggttc acgcaccaac
    52021 accacctggc tgggagaaga agccgcacgc aacactcgta ttctggaacg ccagccgcgc
    52081 gtgataagcc atgaacagca gatgcgcttt agtcagtaag attatcccct tctcctgcgt
    52141 tgacgggcca ggcagtcgtc tggctctgtt tttgcagggc tgcaatctgc gctgcaaaaa
    52201 ctgtcacaat ccgtggacga tggggcgctg taatgattgc ggagagtgcg tgccgcagtg
    52261 tccgcatcag gcgttgcaga ttgtcgacgg caaagtggtg tggaacgctg tggtctgcga
    52321 gcaatgtgat acctgcctga agatgtgtcc gcaacatgcc acgccaatgg cgcagtccat
    52381 gagcgtggat gaggtgctta gccatatccg caaagcagtg ctgtttatcg aagggataac
    52441 ggtaagcggc ggcgaagcca cgacccagct accgtttgtg gtggcgctgt ttactgctat
    52501 caaaaacgat ccgcaactgc gccacctcac ctgtctggtg gacagtaatg gcatgttgag
    52561 cgaaaccggc tgggaaaaat tgcttccggt gtgcgacggc gcaatgctcg atctcaaagc
    52621 gtgggggagc gaatgtcatc agcaattaac cggacgcgat aatcagcaga tcaagcgcag
    52681 catctgtttg ctggcagagc gcggtaagct ggcggaactg cgtttgctgg taatccctgg
    52741 ccaggtggat tatttgcaac acatcgaaga actggcggcg ttaatcaagg gacttggcga
    52801 tgttccggta cgcctgaacg cgtttcatgc ccacggcgtg tatggcgagg cgcaaagctg
    52861 gccgagcgcc acgccggagg atgtggaaca actggctgat gcgttatggg agcgcggggt
    52921 gagccggtta atatttccgg cgctatattt gtgacatgcc ggatacggcc tacggatcgg
    52981 cacagacctg taggcatgat aagatgcgtc aagcgtcgca tcaggcattg tgcgccagct
    53041 gccggatgcg gcgtgaacgc cttatccggc ctacggttcg gcacagactt gtaggcatga
    53101 taagacgcgt caagcgtcgc atcaggcatt gtgcgccaac tgccggatgc ggcgtgaacg
    53161 ccttatccgg cctacggcac gttaaacaac gtatacgtat tatttagcaa cacttcggca
    53221 atctcatctt ctggttccgg gcgtaactcg caaagtacgt caaacacccg cgcagcctgt
    53281 tccgggcggt taggctgccc ctgaaagccg ttgagcggca tatccggcgc gtcggtttcc
    53341 agcaacaacg atgccagcgg taattttgcg atgacatcgc gggttttact ggcgcgtgga
    53401 taggtgatag taccgcctac gccgattttg tagcccagca gtacaaacct ttcggcctgt
    53461 tgcaggctgc cggaaaaacc gtgaaccacg ccagtgcgtg gcaaatcgtg gcgtttaaga
    53521 tgcatcgcca gtttgtcgtg tgtgcgccgt gaatgcagga tcaccggcag atcgtagcgt
    53581 ttcgccagtt tcagttgttc gtcgagtaac cactgctgcc tctcaaattg cggatcgtcg
    53641 ccgaagagat ccagaccgat ctcccccacc gccaccacct tcgccggacg cctttccagc
    53701 gcctgctgta gctgatcaag agacacatcg ctatgttttt tcaacatacc aggatgcaag
    53761 cccaatgcgg catacagcgg ttgatatttt tccgctaacg cctgcacgcg ggcaaaattt
    53821 tccgcctctg tcgccggaac aatgatcttg cctacgcccg cttgtgccgc gcgttgcagg
    53881 ctggcctctt catcaccact aaaaggcggg aaatcaaaat ggcagtgggt gtcgataaaa
    53941 cggcaaatca agccagatcc tcattgttaa atgtcgggtc gttcgcctgc ggagcgtcga
    54001 tcacttcggc aaccagcgta tcgttggcga ctggcgcagg cggaatgaca atcgattccg
    54061 gcgtcaccac cggcaccaga tggcgggtaa gcggcgcttt ttcagtcaat aacttaccga
    54121 ccgttgccag aaaataacga ccgcataaac gcccaagttt atagtcttca cgcagcgccg
    54181 gaatccgact gccaagcgcg atactatgca acggcttcgg cggataaatt tcgaatatcc
    54241 gcagctggcc cggtggtttc tcaataaatt gctgaatgtc acgatagctg gtttcatgat
    54301 gctgcaccag attgaccagc ggctgcaggc tactgtcacc cagccagcgt tccatgcgtt
    54361 tgaaccactg cggcgtgtag tacatttgcg acggcacggt gcggatgacg accaaggttt
    54421 ttgctccctg ccttgccgcc tctttaaccg gaatcgcatc actgatcccg ccatccaggt
    54481 agttaatgcc ttccagcgac actccgctac gataaaagcc aggtatcgca ctggaggcgc
    54541 gaatcacatc cagccagttt tgtttggttg gtaaaaagta attcggcgca tagtcatcct
    54601 gacgacaggc gcacatataa aacgatttgc cgctgtcaaa caaccgcgcg gcggtatcca
    54661 tttgcaacgg catctggctt gcagtggcct ccaccagcca gtcgagatcg ataagatttc
    54721 ctccacggac aaagcgcaat ggatcgaaaa attcgcgttt tgtggtatag cgcatgatga
    54781 ctttgcgcgc gtaaccgggc tgattgcaga tatacgccga gaggttctgc gccccggcag
    54841 atgtgccgag ataaagatcg aaaggattaa actgcgcgcg cataaactca tccagcacgc
    54901 cagccgtgaa aattccacgc tgtccgccgc cttcgcacac cagagctatt cgtccaggct
    54961 ggaacggcct tagcgacaac ggcgcaatat taccaagcgt tacaggtatt cgctgcccca
    55021 cctctgcctt cctgtttttg ttgtgaaaga atgacaaagt aacgcaattt attattcgct
    55081 aaaaccgcaa aagccagtcc gctggactgg ctttggataa aattttctta atggtctgaa
    55141 atctagggtc gttttcggcc catgaacaaa ctcaccagga acagaataat cccgacgaca
    55201 aagacaattt tagctgcgcc tgcagcggta ccggccagac caccaaaccc aagtgcggcg
    55261 gcgattaacg cgataaccag aaatatgatg ccccaacgaa acataagcgt ctcctttacc
    55321 atagttaatg tcaccgcttg atatgagcgt gaaaatcact caatgcgata tttttagtgt
    55381 ggtgcacatc gcgcctcccg acaaagttcg ggaggacgaa ttacgacgaa ttacttagtt
    55441 ttcagatcat ttttaacgct tttcacacca tctaccgctt tggcgatact ttcagcacgg
    55501 tcgctctgtg cctgggaatc gacggtaccg gagagctgaa ccacgccgtc ggtggtttca
    55561 actttcacat gacgggaagg gacgatatcg tccgccagca gtttggcttt gatttcactg
    55621 gtggtggcgg tgtcacccgc gtagcccttc accgagcctt ctttagcgtc gcgaacgtgc
    55681 agtttgtcgc tgacagaggt caccccttca acgcctttcg ccactttcac tgcctcttcg
    55741 gcctgagcct ggctttcaac gaaaccgctc agggtcacga ctttttgatc ggttttgaca
    55801 gagatatcgg tgctcttgat gttgtcatga tccaccagcg ccgccttcac tttcgcggtg
    55861 atggcgctgt catccatgaa attaccgact ttattcatag agctatcgac tttttgccct
    55921 gcgctttcat tggtagtctg tgcgttgttt tccgcgtagg cagagccggt cgcgacggca
    55981 gaggtcaaca ttacagccag cagagttttc gaaatcttca gtcttgtcat agtcatcgat
    56041 ttattcctgt atgtttgctc gtaatttgag cctggcaaca cgaggttgca ttgctgaata
    56101 gggagagact tcaccctcta cagaagtcaa tggccgccat cacaaaagcg atgggtgatg
    56161 aataacgacc attacagcct ctgaatcagt tattaatatc ggtagaatga caatcgacgg
    56221 ctttagatac tgatatctac gcattgaacg gtatttaacg ccgtcagaaa tgtcattact
    56281 ttgttaaata tagatcacaa ttttgaaacc gctcgggata tcagcgaaaa cataagcaaa
    56341 agtgaatgtt ttaagaacat tccgtaagcg gctaataagg aagggaaatt gacagggcgc
    56401 agcggctacc gcgccctgga ggcaagaatt aatgctcgcg ggtctggtgg aactgaacgt
    56461 ctggataacg ttcctgcgcc aggcgcaggt tgaccatgct ggtagcgatg taagcgaggt
    56521 tatcgccgcc atcaagcgcc agttggcttt cgttcttacg cttgaactct tcgaatttct
    56581 tcgcgtccgc acattctacc cagcgggcag tggcaacgtt gactgactca tacactgctt
    56641 caacgttgta ttcgctcttc agacgcgcta ccaccacatc aaactgcagc acaccaaccg
    56701 caccaacgat cagatcgttg ttggagatcg gacggaacac ctgcaccgcg ccctcttcgg
    56761 aaagctgtac cagcccttta agcagctgtt tttgcttcag cggatctttc aggcggatac
    56821 gacggaacag ttctggcgcg aagttcggaa taccggtgaa cttcatcatc tcaccctggg
    56881 taaaggtgtc gccgatctga atggtgccgt ggttgtgcag accaaggata tcgccaggat
    56941 acgcttcttc aacgtgcgaa cggtcacccg ccataaaggt cagcgcgtcg gagatcacca
    57001 catctttcgc agtacgcacc tggcgcagct tcatgccttt ttcatattta ccggacacca
    57061 cacgcataaa cgccacgcgg tcgcggtgtt tcgggtccat gttggcctga attttaaata
    57121 cgaagccggt aaatttgtct tcgctcgcct ctacggtacg ggtatcagtc tgacgcggca
    57181 tcggcgcagg agcccactcc accaggccat ccagcatatg atcaacgccg aagttaccca
    57241 gcgcagtacc gaagaatacc ggagtgattt cgcccgcaag gaacagctct ttgtcgaact
    57301 cgttagacgc gcctttcacc agctccagtt cgtcacgtag ctgctgtgcc agatcttcac
    57361 caaccgcagc gtcgagatcc gggttattca gccctttaac aatgcggact tcctgaatcg
    57421 tgtggccttt accgctctga tagagatagg tttcatcttt ataaaggtgg taaacgcctt
    57481 taaacagctt gccgcagcca atcggccagg tgatcggtgc gcagccgatt ttcagctcgt
    57541 tctcaacttc atcgagcagc tccatcgggt cgcggatatc acggtcaagt ttgttcataa
    57601 aggtgaggat cggcgtgtcg cgcagacggg taacttccat cagcttacgg gtacgatctt
    57661 caacaccttt tgcggcgtcg ataaccatca ggcagcagtc caccgccgtc agggtacgat
    57721 aggtatcttc cgagaagtct tcgtgccccg gggtgtcgag caggttaacc aggcaatcgt
    57781 gatacggaaa ctgcatcaca gacgtagtaa tggagatccc acgctgcttt tccatctcca
    57841 tccagtccga cttagcgtgc tggttggaac cacggccttt tactgtaccg gcgctctgaa
    57901 tggcctgtcc gaacagcagc accttctcgg tgatggtagt cttaccggcg tccgggtgag
    57961 aaataatggc aaaagtgcgg cgcttcgcca cctcttgcaa ataaggagac aacgtcataa
    58021 ttctttcttc ttgagtaagc ggcatcgcgc cgcgcttgtt gaaaacgaaa aattgcggct
    58081 attttaccca tcaacggggg ggaggcaatc agtgtttaca caggagctgc tccagttcgt
    58141 gcaacgaaga aacggtccag gtgggcgcga tgccttctgg ttgctcgcga ttgtgcgcat
    58201 tcagccagca ggtcgcaagc ccggcgttga tgccaccgag aatatcggac tcagctgtgt
    58261 cgccaaccat cagcacacgt gaacggtcag gattgcctgc ctgttccagc gcataatcga
    58321 aaattttctt attcggtttg gcaacgccaa cttcttcgga aatcaccagc aaatcgaaat
    58381 aatcacgcag gcccgtgcgt tccagacgca cttgctgcaa ggcgctaaag ccgttggtga
    58441 tgatgccaat tttagcgttg ccacgaatgg cgttaagcag agaaaccgcg cccggcagtg
    58501 gcgtacagat ttccgccatc gcattaataa aggcttcgtt gagtttaccc ggctcgacgt
    58561 tcagccgttc ggcccagctc tcaaaccgcc cgtgctgaag ctgtaatgaa gtgatcgcgc
    58621 cgttttgata atccacccac agtggcttgt taacggcctg atagtcctga aaatcttcag
    58681 cggtaaaggt gacgctgtaa tcaagaaaca tccgctgcaa gccggtgaat gagtcaaagg
    58741 taaacagcgt ttcatcggca tcaaagaaaa tccagtccca cttcattatt ccaccttgtc
    58801 ttacatactg attggcaacg ccatgatgat ggcgtcttcg cgaccgtccg tggtggggta
    58861 gtaattgcgg cgaattgtcg cctcgttaaa gcctaaactt tcgtacaggg caatggcggc
    58921 agcgtttgaa gcacggactt ccagccatag cgtcgccacg ccgcgttttt ccagttcgtc
    58981 gatcagatgt tccagcagcg cccttcccaa tccctgacgc tgatagtcag gatcgaccgc
    59041 aatattgaac aatgtagctt catccagcac cacttgcgta atcgcaaacg ccgccatttt
    59101 gccgttttgc gttaactgaa agttgagata acgctcgccc tggttgctgg caaaggtttt
    59161 ttcactccac ggaaaggcgt gggcgcgttg ttcaatgtgg taagccgctg gtaaatcagt
    59221 cgtttcgagg gaagaaatcg tgttcatatg tgcaaatttg ttgccataac gcggcgcgtg
    59281 ccgttgggtt tgcccgtaat tcggtgagcg ccggtgatgc cacctgagcg ccttccagtg
    59341 atagcggttc gtcagtaccc aaccgccagc tgttgcagcg actgccttgc ggcagcatcg
    59401 cgattttttc tggcgtcagt tgcagcactt gatcggggct gacggttaac gcgcgcagaa
    59461 catcgctcac tataggatcg gtcagggcgg gaagatcgtt tgccaccatc accagacgga
    59521 cgtgtgccgg gatcgcaatg gcaatctccc cctgcaacgc gccagggcga cgcagcgacc
    59581 actgggtaat gcccagttgc tgtaactgcc agtctcgtcg ggatgtcata gcgaatcgct
    59641 cctgttgtca ggggcgcaaa tatagcaaat tcgtcgatac cgcgccaaca tatggctata
    59701 atcgccgcca gtattaattg aggagcattc catgtctgca tttaccccgg caagtgaagt
    59761 cttgctgcgt cacagtgatg atttcgaaca aagccgtatt ctttttgccg gagacttaca
    59821 ggatgacctg cccgcgcgtt tagataccgc ggccagccgt gctcataccc agcaattcca
    59881 ccactggcag gtgttaagcc gccagatggg ggataacgcc cgttttagtc tggtcgccac
    59941 ggcggatgat gtcgcagatt gcgatacgct gatttactac tggccgaaga acaaaccgga
    60001 agcccagttc cagttgatga atttactttc tctgctgcca gtgggcacgg atatttttgt
    60061 cgttggcgag aaccgcagcg gcgtgcgcag cgccgagcag atgctgtcag attatgcgcc
    60121 gttgaataaa atggacagcg ctcgtcgctg tggcctctat tttggtcgtc tggaaaaaca
    60181 gccagtattt gatgccgata aattctgggg cgaatacagc gtcgatggcc tgacggtcaa
    60241 aacgctgcct ggcgtgttta gccgcgacgg tctggatgtc ggtagccagt tgctgctctc
    60301 gacgttaacc ccgcacacga aaggtaaagt gctggatgtc ggctgtggcg cgggcgtact
    60361 ttcggttgcc tttgcgcgcc attcgccgaa aattcgtctc actctgtgcg atgtctctgc
    60421 accagcggta gaagccagcc gcgcaacact tgcggccaac ggtgttgaag gtgaagtctt
    60481 tgccagtaac gtcttttccg aggtgaaagg tcgttttgat atgatcatct ccaacccgcc
    60541 gttccacgat gggatgcaaa ccagcctgga tgccgcgcaa acgctgattc gcggcgcggt
    60601 gcgtcatctt aatagcggcg gcgagctgcg aattgtagcg aacgccttcc tgccttatcc
    60661 ggacgtgctg gatgagacat ttggcttcca cgaagtgatc gcgcaaaccg ggcgcttcaa
    60721 ggtgtatcgc gccattatga cccgccaggc gaagaaaggt tgagtatctg atatatgtcg
    60781 ccgcataccg tgtcggatgc ggcgtacacg ccttatctga ccgacaaacg aattatccac
    60841 aatccgctga tggacatttt tccagcaatt acacctctgt cgataattaa ctattgacga
    60901 aaagctgaaa accactagaa tgcgcctccg tggtagcaat tctttttaag aattgacggt
    60961 atgcgaaggt ggcggaattg gtagacgcgc tagcttcagg tgttagtgtc cttacggacg
    61021 tgggggttca agtccccccc ctcgcaccaa aaaccacgtt gatattgctc gcactgggcg
    61081 aaggtggcgg aattgg
//
LOCUS       DAZGHF010000013        60138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGHF010000013 DAZGHF010000000
VERSION     DAZGHF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.013"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            51..791
                     /gene="yiaT"
                     /locus_tag="ACHK3A_000899"
     CDS             51..791
                     /gene="yiaT"
                     /locus_tag="ACHK3A_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO8739026.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPTDNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(912..2891)
                     /locus_tag="ACHK3A_000900"
     CDS             complement(912..2891)
                     /locus_tag="ACHK3A_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO8739027.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNAYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHTVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKT
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHCHPEVG"
     gene            complement(2902..4302)
                     /locus_tag="ACHK3A_000901"
     CDS             complement(2902..4302)
                     /locus_tag="ACHK3A_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8739028.1"
                     /translation="MTSTPITAADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTTSSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            4528..5343
                     /locus_tag="ACHK3A_000902"
     CDS             4528..5343
                     /locus_tag="ACHK3A_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8739029.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            complement(5375..6070)
                     /gene="araD"
                     /locus_tag="ACHK3A_000903"
     CDS             complement(5375..6070)
                     /gene="araD"
                     /locus_tag="ACHK3A_000903"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO8739030.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDEARQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6064..6924)
                     /gene="sgbU"
                     /locus_tag="ACHK3A_000904"
     CDS             complement(6064..6924)
                     /gene="sgbU"
                     /locus_tag="ACHK3A_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO8739031.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWTVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(6917..7579)
                     /gene="ulaD"
                     /locus_tag="ACHK3A_000905"
     CDS             complement(6917..7579)
                     /gene="ulaD"
                     /locus_tag="ACHK3A_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO8739032.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPNKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPVQVAGDFHAQIDAIWGGA
                     RA"
     gene            complement(7576..9072)
                     /gene="lyxK"
                     /locus_tag="ACHK3A_000906"
     CDS             complement(7576..9072)
                     /gene="lyxK"
                     /locus_tag="ACHK3A_000906"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO8739033.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPLVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTTQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRVHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(9076..10062)
                     /gene="yiaO"
                     /locus_tag="ACHK3A_000907"
     CDS             complement(9076..10062)
                     /gene="yiaO"
                     /locus_tag="ACHK3A_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO8739034.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGAELLKAVDEVQ"
     gene            complement(10075..11352)
                     /gene="yiaN"
                     /locus_tag="ACHK3A_000908"
     CDS             complement(10075..11352)
                     /gene="yiaN"
                     /locus_tag="ACHK3A_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO8739035.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(11355..11828)
                     /gene="yiaM"
                     /locus_tag="ACHK3A_000909"
     CDS             complement(11355..11828)
                     /gene="yiaM"
                     /locus_tag="ACHK3A_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO8739036.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            complement(11946..12413)
                     /gene="yiaL"
                     /locus_tag="ACHK3A_000910"
     CDS             complement(11946..12413)
                     /gene="yiaL"
                     /locus_tag="ACHK3A_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO8739037.1"
                     /translation="MIFGHIAQPDPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAAENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTTSEIRKIVVKVALTALN"
     gene            complement(12425..13423)
                     /gene="yiaK"
                     /locus_tag="ACHK3A_000911"
     CDS             complement(12425..13423)
                     /gene="yiaK"
                     /locus_tag="ACHK3A_000911"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO8739038.1"
                     /translation="MKVTFEQLKAAFNRVLISRDVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            13624..14472
                     /gene="yiaJ"
                     /locus_tag="ACHK3A_000912"
     CDS             13624..14472
                     /gene="yiaJ"
                     /locus_tag="ACHK3A_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO8739039.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14574..15047
                     /gene="ysaA"
                     /locus_tag="ACHK3A_000913"
     CDS             14574..15047
                     /gene="ysaA"
                     /locus_tag="ACHK3A_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO8739040.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(15199..16452)
                     /gene="avtA"
                     /locus_tag="ACHK3A_000914"
     CDS             complement(15199..16452)
                     /gene="avtA"
                     /locus_tag="ACHK3A_000914"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO8739041.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRIKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMSEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(16630..18657)
                     /gene="malS"
                     /locus_tag="ACHK3A_000915"
     CDS             complement(16630..18657)
                     /gene="malS"
                     /locus_tag="ACHK3A_000915"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO8739042.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALVEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAA
                     SVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            18977..19801
                     /gene="bax"
                     /locus_tag="ACHK3A_000916"
     CDS             18977..19801
                     /gene="bax"
                     /locus_tag="ACHK3A_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO8739043.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(19909..21087)
                     /gene="xylR"
                     /locus_tag="ACHK3A_000917"
     CDS             complement(19909..21087)
                     /gene="xylR"
                     /locus_tag="ACHK3A_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO8739044.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(21165..22346)
                     /gene="xylH"
                     /locus_tag="ACHK3A_000918"
     CDS             complement(21165..22346)
                     /gene="xylH"
                     /locus_tag="ACHK3A_000918"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO8739045.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(22324..23865)
                     /gene="xylG"
                     /locus_tag="ACHK3A_000919"
     CDS             complement(22324..23865)
                     /gene="xylG"
                     /locus_tag="ACHK3A_000919"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO8739046.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(23943..24935)
                     /gene="xylF"
                     /locus_tag="ACHK3A_000920"
     CDS             complement(23943..24935)
                     /gene="xylF"
                     /locus_tag="ACHK3A_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8739047.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            25301..26623
                     /gene="xylA"
                     /locus_tag="ACHK3A_000921"
     CDS             25301..26623
                     /gene="xylA"
                     /locus_tag="ACHK3A_000921"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO8739048.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            26695..28149
                     /gene="xylB"
                     /locus_tag="ACHK3A_000922"
     CDS             26695..28149
                     /gene="xylB"
                     /locus_tag="ACHK3A_000922"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO8739049.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWSMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            28318..28659
                     /gene="yiaB"
                     /locus_tag="ACHK3A_000923"
     CDS             28318..28659
                     /gene="yiaB"
                     /locus_tag="ACHK3A_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO8739050.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGILMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            28704..29141
                     /gene="yiaA"
                     /locus_tag="ACHK3A_000924"
     CDS             28704..29141
                     /gene="yiaA"
                     /locus_tag="ACHK3A_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO8739051.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(29183..30178)
                     /gene="wecH"
                     /locus_tag="ACHK3A_000925"
     CDS             complement(29183..30178)
                     /gene="wecH"
                     /locus_tag="ACHK3A_000925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO8739052.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVCAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAITLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            30352..30660
                     /gene="ysaB"
                     /locus_tag="ACHK3A_000926"
     CDS             30352..30660
                     /gene="ysaB"
                     /locus_tag="ACHK3A_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO8739053.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     ALCKDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            30755..31666
                     /gene="glyQ"
                     /locus_tag="ACHK3A_000927"
     CDS             30755..31666
                     /gene="glyQ"
                     /locus_tag="ACHK3A_000927"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO8739054.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            31676..33745
                     /gene="glyS"
                     /locus_tag="ACHK3A_000928"
     CDS             31676..33745
                     /gene="glyS"
                     /locus_tag="ACHK3A_000928"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO8739055.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNTNVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAASLAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            34067..34219
                     /gene="hokA"
                     /locus_tag="ACHK3A_000929"
     CDS             34067..34219
                     /gene="hokA"
                     /locus_tag="ACHK3A_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO8739056.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMVRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(34407..34619)
                     /gene="cspA"
                     /locus_tag="ACHK3A_000930"
     CDS             complement(34407..34619)
                     /gene="cspA"
                     /locus_tag="ACHK3A_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO8739057.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(34900..35190)
                     /gene="yiaG"
                     /locus_tag="ACHK3A_000931"
     CDS             complement(34900..35190)
                     /gene="yiaG"
                     /locus_tag="ACHK3A_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO8739058.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            35624..36334
                     /gene="yiaF"
                     /locus_tag="ACHK3A_000932"
     CDS             35624..36334
                     /gene="yiaF"
                     /locus_tag="ACHK3A_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO8739059.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(36384..37358)
                     /gene="ghrB"
                     /locus_tag="ACHK3A_000933"
     CDS             complement(36384..37358)
                     /gene="ghrB"
                     /locus_tag="ACHK3A_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO8739060.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTIEQNAAILA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(37462..38121)
                     /gene="yiaD"
                     /locus_tag="ACHK3A_000934"
     CDS             complement(37462..38121)
                     /gene="yiaD"
                     /locus_tag="ACHK3A_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO8739061.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            38274..40607
                     /gene="bisC"
                     /locus_tag="ACHK3A_000935"
     CDS             38274..40607
                     /gene="bisC"
                     /locus_tag="ACHK3A_000935"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO8739062.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVMVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(40576..41016)
                     /gene="yiaC"
                     /locus_tag="ACHK3A_000936"
     CDS             complement(40576..41016)
                     /gene="yiaC"
                     /locus_tag="ACHK3A_000936"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO8739063.1"
                     /translation="MIREVQHSELPAILELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(41013..41576)
                     /gene="tag"
                     /locus_tag="ACHK3A_000937"
     CDS             complement(41013..41576)
                     /gene="tag"
                     /locus_tag="ACHK3A_000937"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO8739064.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            41734..42432
                     /gene="yhjY"
                     /locus_tag="ACHK3A_000938"
     CDS             41734..42432
                     /gene="yhjY"
                     /locus_tag="ACHK3A_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO8739065.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            42661..43869
                     /gene="yhjX"
                     /locus_tag="ACHK3A_000939"
     CDS             42661..43869
                     /gene="yhjX"
                     /locus_tag="ACHK3A_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8739066.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            44193..45884
                     /gene="eptB"
                     /locus_tag="ACHK3A_000940"
     CDS             44193..45884
                     /gene="eptB"
                     /locus_tag="ACHK3A_000940"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO8739067.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            45976..46052
                     /locus_tag="ACHK3A_000941"
     tRNA            45976..46052
                     /locus_tag="ACHK3A_000941"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46010..46012,aa:Pro,seq:cgg)
     gene            46087..46220
                     /locus_tag="ACHK3A_000942"
     ncRNA           46087..46220
                     /ncRNA_class="other"
                     /locus_tag="ACHK3A_000942"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            46965..48572
                     /gene="dppA"
                     /locus_tag="ACHK3A_000943"
     CDS             46965..48572
                     /gene="dppA"
                     /locus_tag="ACHK3A_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO8739068.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(48635..48862)
                     /locus_tag="ACHK3A_000944"
     CDS             complement(48635..48862)
                     /locus_tag="ACHK3A_000944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739069.1"
                     /translation="MTRSLELRVCIAHKSIPALVEQGMKYCCRMALHAEAQRHRVCRL
                     LTKSALFMPDAAQTPYPTYKIVQTQYIAEFS"
     gene            48880..49899
                     /gene="dppB"
                     /locus_tag="ACHK3A_000945"
     CDS             48880..49899
                     /gene="dppB"
                     /locus_tag="ACHK3A_000945"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO8739070.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            49909..50811
                     /gene="dppC"
                     /locus_tag="ACHK3A_000946"
     CDS             49909..50811
                     /gene="dppC"
                     /locus_tag="ACHK3A_000946"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO8739071.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            50822..51805
                     /gene="dppD"
                     /locus_tag="ACHK3A_000947"
     CDS             50822..51805
                     /gene="dppD"
                     /locus_tag="ACHK3A_000947"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO8739072.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            51802..52806
                     /gene="dppF"
                     /locus_tag="ACHK3A_000948"
     CDS             51802..52806
                     /gene="dppF"
                     /locus_tag="ACHK3A_000948"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO8739073.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(52836..54107)
                     /gene="yhjV"
                     /locus_tag="ACHK3A_000949"
     CDS             complement(52836..54107)
                     /gene="yhjV"
                     /locus_tag="ACHK3A_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO8739074.1"
                     /translation="MQHNTLPKHDQKMPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            54583..54690
                     /locus_tag="ACHK3A_000950"
     CDS             54583..54690
                     /locus_tag="ACHK3A_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8739075.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNWLNKRK"
     gene            55066..55173
                     /locus_tag="ACHK3A_000951"
     CDS             55066..55173
                     /locus_tag="ACHK3A_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8739076.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            55549..55656
                     /locus_tag="ACHK3A_000952"
     CDS             55549..55656
                     /locus_tag="ACHK3A_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8739077.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            56032..56139
                     /locus_tag="ACHK3A_000953"
     CDS             56032..56139
                     /locus_tag="ACHK3A_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8739078.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(56226..57905)
                     /gene="bcsG"
                     /locus_tag="ACHK3A_000954"
     CDS             complement(56226..57905)
                     /gene="bcsG"
                     /locus_tag="ACHK3A_000954"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO8739079.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDITEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(57902..58093)
                     /gene="bcsF"
                     /locus_tag="ACHK3A_000955"
     CDS             complement(57902..58093)
                     /gene="bcsF"
                     /locus_tag="ACHK3A_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO8739080.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(58090..59661)
                     /gene="bcsE"
                     /locus_tag="ACHK3A_000956"
     CDS             complement(58090..59661)
                     /gene="bcsE"
                     /locus_tag="ACHK3A_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO8739081.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            59934..60122
                     /gene="bcsR"
                     /locus_tag="ACHK3A_000957"
     CDS             59934..60122
                     /gene="bcsR"
                     /locus_tag="ACHK3A_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO8739082.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT        14709 a        15002 c        15690 g        14737 t
ORIGIN      
        1 cattactatg ccgactcttt tttatcttat tttaaaatgg aataccggac atgttaatta
       61 accgcaatat tgtggcgtta tttgcgttgc cttttatggc aagcgcaact gcttctgaat
      121 tatccattgg tgctggtgcg gcttataatg aatcgcctta tcgcggttat aatgaaaata
      181 caaaggcaat tccgctgatt agttatgaag gtgatacttt ttatgttcgt cagaccacgt
      241 taggttttat tctgtcgcaa agtgaaaaaa atgaacttag cctgaccgca tcctggatgc
      301 cgctggaatt tgaccctacc gataatgacg attatgccat gcaacagctt gataagcgtg
      361 atagtacggc tatggcgggg gttgcctggt atcaccacga gcgttgggga accgtgaaag
      421 cctctgcggc tgcggacgtt ctggataaca gcaacggctg ggtgggggag ctatcggtat
      481 tccacaaaat gcagataggt cgcctgtcgc tgacacctgc gctgggcgtt ctctattatg
      541 acgagaattt cagtgactat tactatggca tttcagagag tgagtcccgt cgtagcggtc
      601 tggcaagtta ttccgcgcag gatgcctggg tgccttatgt cagcctgacg gcaaaatacc
      661 cgataggaga gcacgtcgta ttgatggcga gcgccggata cagcgagctg ccggaagaga
      721 ttaccgacag cccgatgatt gatcgtaatg agagttttac ctttgtcacc ggcgtgagct
      781 ggcgttttta atttaccggt ggatgtcggt gcggctggcc tacgctctcg caccgactcg
      841 tgggcctgat aagacacgtt aagcgtcgca tcaggcaatg tgctccctac gttggatgcg
      901 gcgcgagcgc cttatccaac ctccggatgg caatgctttt cctcattcac ccagatccgc
      961 atttcgcctt caccccggtt ggcccagcta aaccacggga taaaggtcag ggtttgcggc
     1021 tggcgtttgg ctggcgcgct gtcgtaatgc cacagcggtt gctgctctgg attgctctgt
     1081 tcataccgat aacccggtgc ctggattaag atcttatggc ggaacaatcc cttaccttca
     1141 aatgtcgtaa atggcgcatc ggcgggcagc cacagattat gcagtgactc gccgttgtcg
     1201 gcctgttcca ggcaatacac cagcgggccg cgctgaatcg ccacttttcc ggcgacgtga
     1261 cgcaccagag ggttaccgta aacgcgacgt accggcattg gcaaagtcag attcagcgtg
     1321 tcgccctcct gccattcgcg ggtaatgtgc aaataccctt tacgaatatc ctgctcgacc
     1381 tcttccccat tcaatatgat ctgcggctgt gtgcaccagt ccggcagacg taaagccagc
     1441 gtatgacgca ccggctgggg cgattcaacc gcaattgtca cctgttcctg ccacggatag
     1501 tttccgctaa cccgcagacg cagcgtgcca ttttctaccg gcacttccat gctgtttcct
     1561 gcgtagatgt tgatatacaa cgcatcttca cgcggcgtgt agagataatg accaatcgag
     1621 gttagcacgc gggcgatatt tggcggacaa caagcgcaac caaaccaacg ctggcggatc
     1681 ggcttaacgt gatcgtagat atggttgaat tttaacgttt ttggatgcac ttccagcgga
     1741 ttaacgtaga agaaatgttt gccatccagc gccatgccgc cgagcacagt gttgtacagt
     1801 gcgcgctcca tcacatcggc atattgactg tcgccttcca tttccagcat tcgccgggcg
     1861 aacatcatca ggccgatgga agcacaactt tcggcgtaaa ccgtgtcatt cggcagatcg
     1921 taatcgctgc tgaacgcttc gccgctgctt tgtgagccaa tgccgccggt aatatataac
     1981 tgacgctggg ccatattgtt ccacagccgc aggcaatcct gacgcttgct ttcatcgtga
     2041 cttaaacgcg cgagatgtgc gacgccagtc atcagatata caaagcgtac tgtatgtccg
     2101 atggcggttt gttgctgtgc gatgggcaaa tgcgcctggc tgtaggcttt atctttcacc
     2161 atccacgccg ggccgtaggt gtgccagtgc gaggtctgcc cgcgcttttc atattcctgg
     2221 tcgtaatagt gcggttgcgc accacgctgt tcgacaaaat agttcgtcag cgccaggtag
     2281 cgcggctctt cggtcacttc atacaggcgc attagtgcca gttcaatttc cgggtgacca
     2341 ggataaccgt gtaacttact ttcacctgga ccaaatacgc tgtcgatatg atcggccaga
     2401 cggcaaacca cttccagcaa gcgccgtttt cccgtggcct ggaagaaggc gactccggct
     2461 tcaatcagat gaccggcgca gtaaagttca tgacactccg ccagattgct ccagcgttct
     2521 tcgggcgctt ttaccgtaaa gtaagcattg agatagccat cttcgcactg ggcggaggcg
     2581 atcagttcaa tcacctcgtc agcggttttt tccagttcgg catccggctt ctggcacagc
     2641 gaccaggcta ccgcttccag ccatttggcg acgtcgctgt cctgaaacac catcccgtaa
     2701 aattcaccgt cttgcagtcc tgcggcaatg cgaaagtttt caatcgcatg gctgggttcc
     2761 gcttctggga tacgatcgtt caaggcgtcc cactgataag gaatcaccac atcgcggacc
     2821 agttgttggt actgaccgag gaacggatcg ctgaccgtca gtttatgcag atcgacttcc
     2881 gaaatgttca tcttgtgctc cttaaggctg tacatgacgc tgacgcaggt cgctagcaat
     2941 gcgtgacatc atagggttat tgagtttgca aaagcggatc gcccctgcca gcagcaggtg
     3001 gaatagcgca ggcagtaatg tttccatcgc cgtgatcccg ccaagcgaac tggtggtctg
     3061 gttttccacg cctggctggt aggcgacaaa aataaacagc aggctgatga tcccggcgct
     3121 ggaggcccat gccagcttga tgaaaaacag attgaaggcg aagttcatgc cggaagaacg
     3181 tacgcgggtt ttccactcgc cgtagtcatc ggcaaaggcc atcaatgaga agtgcagtgg
     3241 caatgtgaag ccaagaatca cgccattacc gaggatcacc gccagccaca gcgtttgata
     3301 agccgggcca gaaggcagga accacatcaa caccgccagc gcagcgagga gcaggttggt
     3361 gtagtagtaa attttgacgg tatcgaaacg ccgcgttaac gggctgacaa tcaccgagcc
     3421 gataatggag gcgaaggtga ccatggtgaa gaacagcgac gtgtagcccg tactgccttg
     3481 taagacgtag gtaatgaagt acatataccc accgccgcga atattaaaga cgttaatcag
     3541 caggaaagac atgaccagca tcagcagcag ttggtcgttg ttccgcagcc cggcaagatg
     3601 ttcgcgcagg gtaaatttcc ccattgtgga gagtggcacc cgttcacgaa cccagaagaa
     3661 acagcacagg aacatcacca cggcaatggc gcacagcacg ccgacgccca gttgatagcc
     3721 gcgcgcggca ttaccctgac cgaagagatc caccatccac ggcaggccaa ccgaaaccag
     3781 aaatcccgct acgccgcaga gaacgaatcg ccaggactgg caggagatca cttcattgtg
     3841 gcgggtggtc atggtgttga tcagcgcgca gtacggtacg ttgatggcgg tataaccgac
     3901 tgaaaggagc aggtaagtac cgaacgccca ggcgattttt actcccatgc tggcatctgg
     3961 cacggtgaac gtcaggatgc cgataatgcc gatgggaatg gcaatccata actgccacgg
     4021 acgaaagcgc ccccagcgac tttgcgtccg gtcggcaatg acgcccatca ccgggtcgga
     4081 gatggcatca aacacgcgca gtgcaataaa cagcgtgccg accagcgccg gagttaagcc
     4141 aaaaacgtcg gtgtagaaaa aggtgagaaa gttcatgatc aggcaagtta ttacggtgcc
     4201 gcctgcgtca cccaggccgt agcctatttt ttcccgtaca gataacgcat cgtcaatttt
     4261 acctttagcg atatcagcgg cagtaatcgg tgtagaggtc atagatattc ctcgtgaata
     4321 gggagcaatt tttagtttta ttgcaatgac ctgattaatt ctgccgtgaa cgggaatatc
     4381 aacaaggcga gaaaagagta aaaaaaggtg acgattccga cctgaattta aaagtgtgaa
     4441 atggatcagg tgaaattaat gtttttgatt aataatcaaa aggtaatgtg tttaatcatt
     4501 aatgaataag cgggaaatta catatcaatg ctcgaattat ccatgacgtt accgattaaa
     4561 gtgcaaaatg gtggattatt tatttcccgt ggcgtaggga ggcacccggc aaggcggctg
     4621 gagtcatggg aaattatctt tgtcgaaaaa ggtcgattaa cgattcagga agaagagcgg
     4681 atatttctgg tcgatgcggg agagagttta ttactctggc cgaaccgaca gcatgttggg
     4741 gtagaggatt ttcccgccga tctcaaattt tactggctgc attttgaggt taaagcgccg
     4801 gatagcgacc cgcgctggct cactcatctt agcgtgccgc aacaaacgaa ggttgccgat
     4861 ccgcaggcgc tggtggccct gtttcgccag ttcttaagcg aacaggaaaa gcatcaacgc
     4921 actccggcgc tggagtttat tgtgctgctg attttgcagc agctcacccg tgacgcgcgt
     4981 cttgatgaac aggccgatgc cgcaggtacc acgcttgcct ggaaagcgca gaaactgatt
     5041 cgcacgcaat atcatcgccc gctttctacg gcgcttttag cccgcgagct gtactgcaat
     5101 gccgattatc ttgggcgcgt ctaccggcgg gtgtttcatc tgacgttaac agaagcaatt
     5161 catcgccagc gagtgcgcga agccgaaaag ctgttaatta gcgaagcgcg atcgctcact
     5221 gaagtggctg cgttgtgcgg gtttaatgac gttggctatt tccgacagat ttttcgcaaa
     5281 cacaccggtt taacgccagc tgcgtggaag cgacgttaca gcaaggaaca tatcaattcg
     5341 tagtgccggg gcaatgaagc cccggcgtga gggattactg cccgtaatag gcattggccc
     5401 catgcttacg caggtagtgc ttatctaaca gttcgttttg catcgcaggg agctgcggcg
     5461 caagctggcg cgagaacagg cccatatagg cgcactcttc cagcactacg gcattatgca
     5521 ccgcatcggc ggcatttttt ccccaggcga acgggccgtg agaatgcacc agcaccgccg
     5581 ggatttgtgc cggactcctg ccgcgttctt cgaaagtttc aatgatcact tcgccggtct
     5641 gatattcata ttcgccgtta atctcctctg cggtcatctg tcgcgtgcag gggatggcac
     5701 catagaaata atcggcgtgc gtggtgcccc aggcggggag atccagcccg gcctgcgacc
     5761 agatggtggc gtggcgcgag tgggtatgca caatgccacc gatttcggca tagcgacggt
     5821 aaagcgccag atgcgttggc gtgtcggacg agggcttttt gctgccttcc accaccttac
     5881 cgctggctat ctcaaccacc accatatcgt cggcggtcat cacgtcgtac tcgacgccgg
     5941 aaggtttgat taccatccat tgccgcgctt cgtctaccgc gctgacattg ccccaggtga
     6001 acgtcaccag atggtgagcg ggaagtgcca gatttgccgc cagcacgtcg gctttcagtt
     6061 gctctaacat ataaaacctg cctcctgcat acgcgcttca atccagcgcc gcgcctgaat
     6121 aatctccagc accggctctt tggctttttc ggtccacatc tcaatcagga aagaaccgcg
     6181 atagttcagt tcatgcagcg ttttgaagat gccaacgaaa tcgacgcagc cttcgccaaa
     6241 cggcacatca cggaactgtc cggggctttg ctcggtgact ggttgagtat ctttcaggtg
     6301 gatcgcggcg atacggtcaa tgcccagttt cagttcggcg ggaacatcat tgccccaggc
     6361 gctgaggttg ccgacgtccg ggtagacggt gaaccacggc gaggcgagca tctcgtccca
     6421 ctttttccat ttgctgatgg agttcataaa cgcagtatcc ataatctcca ccgccagcat
     6481 tacttgtgat gccgccgcct gttcgactgt ccacgccagc ccttcagcaa aacgttgccg
     6541 ggtgccttcg tcgtggtctt cgtaatagac gtcgtagccc gccagctgga tggtgcgaat
     6601 gccgagatcg cgcgccaggc gaatcgcttt actcatgatt tcccgcgccc gttcgcgcac
     6661 tgcctcgtca cggctaccaa aaggaaagcg acgatgtgcg gacaggcaca tcgacgggat
     6721 cccaacaccc gtttcgatca tcgcagtgac cagcgaagca cgttgtgcgg cgctccagtc
     6781 aagacgtgag agccgttcgt cggtttcatc caccgacatt tcgacaaaat caaagccaca
     6841 gctttttgcc agcaccagcc gctccggcca ggagagatct ttcgccagcg ctttttcata
     6901 aatccctaac ggatgattac gcacgcgcac ctccccagat ggcatcaatt tgtgcatgga
     6961 aatcgccagc cacctgcacc ggatttgctg cgcctgccag tgctcgcccg gcaataaacg
     7021 ctttcacgcg aatatcttta aacagcggca ggtcagcagg ggtaatcccg ccggtaatgg
     7081 aaagctcaag gccgatatcg gaaagcgcct tcatgcgtgc cagatcggcc tcgccccact
     7141 gttgcccgct ggcctgcgcg tcgcgaccgc gatgataaat ggcctgccgc acgccaatac
     7201 gatgccagtc gcgggcgtcg tccagcgtcc agttaccgaa caactcgatc tgaatttcac
     7261 caccgcagcg ttgcgccatc gcgtggcctt tttccaccgt cgcgagcggt gcggcgcaga
     7321 tgatggtcat ccagttagcg cctgcgccaa acgcctgttg cgcgagcgtt tcaccggcat
     7381 cggcgacctt ccagtcagcg acgatgattt tgttcgggca ctgttcgcgc aaggctttca
     7441 ctgcgccaag cccttcgttt aaacagagaa tggtgcccgc ttcgacgata tcgacgctgt
     7501 cttttaacag cgtcacgtcg cgctgtgcgt cttcaagtga tgagtggtcg agggccagtt
     7561 gcagaagtgg tcggctcata atgtgtgctc cttgatgcgg gcgtgaaagc cctgaagtgc
     7621 ggcaatgaga tgctgataac gttggtattt ttgttggtaa agctgatggg cggtcatatc
     7681 cggcagcagg gtgcgtaccg ggtgctgtaa atcacgttgg gcttcgctga aatcgcgata
     7741 aactccggtg ccgacgcggg cggcaagtgc cgcaccaaag cagccggttt cttccacctg
     7801 cggcagctcg atacgcagac cgctgacgtc cgccagcatt tgcatccaga catcggagtg
     7861 cgccggaccg ccagtgacgc gcagggtgtg cacatcagta aaacgttcgc gcatccggtt
     7921 gagatgggtc atatggctga acaccacgcc ttcataaatg gcctgcaaca ggtgtacgcg
     7981 ggtgtgaatg gcctgcatcc cgtagaaacc gctggtcatc tcgagtccag cgttgctgcc
     8041 gtacaggaac ggcaggaaaa agagatcgcc cccggccttc ggcaagctgg caacggcctg
     8101 gttgatctca tcaaacgaga tttctcccca ctgtgtggta aaccattcga gattgccgga
     8161 agaggtgggg cttgcttcat gaacgataaa ttgaccatcg ttaacgtagc gaccatagac
     8221 atacggatgc gcttcaccgt cacgtaaacc acgggttatg ccgctggtca ccgcccaggt
     8281 gcccatcacc gcattgaggg taaattcgtc ttcgatcccg gcgcagagtg cggtggaaac
     8341 cacatcaaac aggccgccga caacgggcgt acccgctttc agaccggtca gtgcggctgt
     8401 ctgagcggtg atctccccgc agatttcggc agatccgaca acaagtggca gggcgtggtt
     8461 gatttcagcg atccccagcc agtcggtgag gcacgggtca tattccccaa gactcatgtt
     8521 gtagagattg gactcagaaa tattgctctc ttcgcaacct ttgacgccgg ttaaacacca
     8581 gcgcaggtag tcgtgcgtca tcatcacgca gccaatttgc gcgtagcgtt ccggttcgtg
     8641 ctctttcagc cagcgtaaca gcgacaccgg atgcccggtc cacaaggttt gtcgcgtcag
     8701 cgggtagagt ttttccggga tgccatcttc ctgccagcga cgaacgattt ccatcgcccg
     8761 gcgatccgag gacaaaatgg cgttaccgag cggcttgtcg tttttatcca gcaaaaacaa
     8821 gccctttccc tgtgcggaga tgccgatacc gacaatttgt tccccgctaa caccagaatg
     8881 agtaagcagg gcgcgaatga cagccatgca gcattgccac agttctgcca tatcgcgctc
     8941 tgcccaacct ggctgcgggc ttaatgcgca cagcggcagg cgctgcacgc ctgcctcccg
     9001 gccttcgcgg tcatacagcc cggctttcag ccagctaccg ccacaatcta accccagcca
     9061 gtattgcgtc atggtttatt gcacctcatc cacggctttc agcagctcag cgccctgcgg
     9121 aacatcttta acaaacatgt cgcggacctg agtacccagt gcgtcgctaa aggctttgcg
     9181 gtcgagatcg gtgatgactt ccacgcccgc ttctttcatg ccgtcgatga ttttttgctg
     9241 atcttcggca accagtttgc gctggtagtt acccgcttcc tgtgcagatg aaactagcgc
     9301 ctgctggaac tccgggctta agccatcaaa cttcgctttg ttgatcacca ccagaagtgg
     9361 cgaataggcg tggtgcgtca gagaaaggta cttctgtact tcgaaaaatt ttgctgacca
     9421 gacgacgttg atcgggtgtt cctgagcgtc gatagtgcgc gtttccagcc cggtatagac
     9481 ttcggcaaac ggcatcggga tcgggttagc gccaaagact ttgaatgcgg cgatattcat
     9541 cgggctattg ttggtgcgaa ttttcagccc tttcaggtcg gcgggggttt taaccggtgc
     9601 gcgcgagttg gtgacatcgc gccagccgtt ttcccagtag gccagtactt ttagtccttt
     9661 accttcaagt gaggctttca gatcatcacc gactttgccg tcgagcgttt tatgcgcgtg
     9721 agcggtatcg cggaacagga aagggacatc aagcaagttc atcactggtg ataacccggc
     9781 aaagttattc gagccggaca tttccatatc gatggtgccg ccacgtacgc cgctgatcat
     9841 cgcctgtgcg ttaccgagag tactgtccgg gaacagtttt aatttcagct cgcctttggt
     9901 tctctcctgc aataaatcat tgaatttttt cgccgcaata tgttgcgagt cggtttgtga
     9961 ggtttcataa ccgaaacgta aagattgcgc cgccagtgaa gatgtactaa atgcagccag
    10021 gccagcaatg aataatgcgt aggttacaga gcgtaatttc ataatatttt ccttttaatt
    10081 aatccatttc aaagggagga tgatgagatc gggaataaaa acaaatacga ctaataatga
    10141 atataaaacc aggacgtaag ggaaaacgcc tctgactgca tcatcgaatt tgagttttgc
    10201 cacccctgaa ataacgttaa gcacattacc gataggcggt gtaattaagc cgattgagca
    10261 gttaatgata aacatgacac cgaaataaat cggatcgatt cctgcctctt taactaaagg
    10321 cattaatacc ggggtaagaa ttaataccgt cggcgttaaa tccatgacca tgccgacaat
    10381 caggatagcc accataatga cgataaacag cagacgcggt gaatcgacca gcggttgcag
    10441 taaatcagaa accatcatcg gcagttcagc aatggtgatc agccaggcgg aaacttgtgc
    10501 cgaggctacc agaaacatca ccactgaggt cgttttcgct gcgccaatga gcacatgcca
    10561 aagtgtggcg aaggtcattt cacggtaaat aactgtggcg acaaacagcg catagaaagc
    10621 agcaacggcc ccggcttcgg taggggtaaa cagaccggag cgaaacccgc caataatgat
    10681 gactggaaga aacagcgccc agataccgga gacaaaagag tgccagattt cctgcatcgt
    10741 tgctttttgc tggcggggta aattaaggcg actggcctgc caccaccagg taagcatcag
    10801 cgttgcgccc atcatcatgc caggggcaat gcccgccata aacagcttac tgatggataa
    10861 tccgctggaa acgccgaaaa taataaacgg aatggaaggg ggaataattg gcgcgataat
    10921 gccgccagag gcaatcagcc ccgcagcccg gttgaccggg tagttagcgc tgcgcatcat
    10981 cggcactaat agcgcggcaa cggcggcggt atcagcaaca gcagaaccag aaaggctcgc
    11041 cataatcata gccgccagca cgccgacgta gcccaacccg ccaggtttat gccccaccag
    11101 tttcatcggc aggtcaacaa tgcgttttga caagccgccc gcattcatga tttcacccgc
    11161 cagcacaaag aacggaatcg ccagcaggga gaagctgtcg gctccgttca ccagcgtttg
    11221 tgccatgatc tggacatcaa acatgtccag ccagaacatt aacgccgctc cgcacaacaa
    11281 cagtgcccag gcaataggca aaccgatagc aataccaccc aacagacagc ccagaaaaat
    11341 cagcacagcc atgattaagc tccttgcggt ggcgacgtta gagaattgct acgcgtgatg
    11401 agttgatata aatgacgcag ttcaaagaat gcgataacga ggctggtggg cagacaagcg
    11461 gcatacatca ggccgatggg caaaccgagg atcggtgaat aatcgctcca gtcctgaatt
    11521 gtttttagcg ttgcgcccca ggccagtgcg ccacaaataa ataagattaa ggaatgggta
    11581 accagagcga ctcgtcgctg ccatgcgggg gagagtttct ccaccagaaa ggtgacctga
    11641 acgtgggcgt tatccataaa agccacaatc gcgccaataa atgttaacca gacaaataaa
    11701 taacgtgaca attcatctac agataaaatg cttgtctgaa aaccatatct taaaataatg
    11761 tttataaaca caatacagga aagtacggcg agattaatcg ccagtattgc ttcaagtatt
    11821 tttttcatag ctattccttg aggctatgtc tgtcataatt caagttgcat gtgcagcaac
    11881 ctaaattatt aatatagtta gagcaataac attacccagg cgttctggcg tagacgatta
    11941 ttcgattaat tcagcgctgt taatgcgact ttaaccacaa tttttcgtat ctcagaggtt
    12001 gtttgcagaa tacaacctgg tcggtgaaca tcctgcggga agaaaatagc gtagctgccc
    12061 ggtatcattt ctataaatga ttcatgttca ctatcgtgat aaaaaataat atcgcgctgc
    12121 tctaatagtg attcgctgac tttattattt cccgtatcaa tagcaatgcc gattttctct
    12181 tcaccccacg ccagaaactg aatatcgata taccgacgat gcacttccgg acggttttcc
    12241 gccgcttcgc gtgtggttaa atcgataatt tgcgcataaa tattcttgcc gtcgatttcg
    12301 acaacgcccg gttccagggc gttgaagtcg gtggtgcgca gaaaatcaag cgccttttca
    12361 atggcggcag gcaaacggca cgggtcaggc tgcgcgatat gtccaaaaat catgggttat
    12421 ctccttataa cgcttggatt tttgcccaca cgctgtcatc aacggtaata ccgttacggc
    12481 ggttttcggc cagcagggta gtaaattcgt ggccgggtaa gcgaatggcc tgattttcgt
    12541 cagcgcgttc agcggtggta acgtaatcca tgatgcgttg cagtttggca tcgcgggtgg
    12601 ggccgtcgat aagcttatct acttcaatgg caataaaaat ttgtgaaatg ccgtattcgt
    12661 cgctgttgtc ctgggtgact tcggcaaccg atgcgccgtc agaaaggaga gtggcgatca
    12721 tatccagcac aatcgacatg cccgaacctt tccagtaacc catcggcaaa atgcggcgat
    12781 tcttctcgat aacgccaggt tctttggtca aattgccctc atcatcaaag ccaccatcga
    12841 ccgggagctg acggcctgcc agacggttaa cttctaacat gccgtaagag aacatcgaca
    12901 tcgacatatc gaccatggtg attggggtag aaggaatggc gacgatcagc gggttagtgc
    12961 cgatgcgaca ctcttttgcg ccccacggcg gcattacggc gatggagttg gtccagcaaa
    13021 tgccaatata gcctttttcc gccgcctgcc agccgtagct gccgccgcgc atccagtggt
    13081 tggcattacg tagtgccacc agaccaatac cgtgatcggc agccagttca atggcgcgat
    13141 ccatcatctt tttcgctgtc aggttaccga tcgaacgctg ggcgtcccac tgttcaattg
    13201 cgccgaggct ggttatacgt ttgggttggg catcaggaat gatatcgccg ttttccagtt
    13261 gttgaatgaa acgagggaaa cggttgacgc cgtgagaata aacgccggat tcggtggtgc
    13321 gggcgaacat ttcggcacag gcatcagccg tttcgctgtc aacgtcgcgt gaaattaaga
    13381 ctcgattaaa ggctgctttt aactgctcaa atgtcacttt catccctggc ttccttgttt
    13441 ttttgatcgc ttttttggct taaaatttca atatgcgaaa tctgatttca aatatatcga
    13501 tcgcttttta acaaagcaat ctgattgatg aatttcaaaa aacttaaaaa tcaagtggtt
    13561 ataaattatc tgtcgagatc gtgaactacg acacactttg cgctaccatc ttgacgcgac
    13621 aaaatgggga aagaagtgat gggaaaaaaa gagaacgaga tggcgcagga aaaagagcgt
    13681 ccagccggaa gccagagtct gtttcgcggg ttgatgctga ttgagatttt gagcaactat
    13741 ccaaacggtt gtccgttggc gcatctttcg gagctggctg gtttaaataa gagtaccgtc
    13801 catcgcttat tgcagggatt acaatcctgc ggctatgtga ccaccgcgcc cgccgcaggg
    13861 agttatcgcc tgaccaccaa atttattgcc gtcgggcaga aggcgctgtc ttcgctgaat
    13921 atcattcata tcgccgctcc gcatcttgag gcactgaaca tcgccactgg tgaaactatt
    13981 aacttctcca gccgcgaaga cgatcacgct attttgattt ataagctgga acccacaacc
    14041 gggatgctgc gaacccgtgc ctatattggc cagcatatgc cgctctactg ttccgcaatg
    14101 ggcaagatct acatggcgtt tggccacccg gactacgtga agtcatactg ggaaagccat
    14161 cagcatgaga tccagccgtt aacccgcaat accattaccg agctgcccgc gatgttcgac
    14221 gaactggcgc acattcgtga aagcggagcg gcgatggaca gagaagaaaa cgaactcggc
    14281 gtctcctgta ttgctgttcc ggtgtttgat attcatgggc gggtgccgta cgccgtgtcg
    14341 atttcgcttt cgacatcacg tctgaaacag gtgggggaga aaaatctcct gaaaccactg
    14401 cgtgaaaccg cgcaggctat ttctaatgaa ctgggattta ctgtcaggga cgaccagggc
    14461 gcgatcacat aacgctttgg acaagtgcca aaactttaac atttccttcg ttggatcaaa
    14521 gcagtagaga cgcgctctct ggcactctgc tgttttagtg caaaggagtg atcatgaacc
    14581 ggtttattat tgcggatgcg acgaaatgta tcggttgccg tacctgtgaa gtggcttgcg
    14641 cagtgtcgca tcaggagaat caggattgcg ctgcgttgtc accagacgag tttatttccc
    14701 gtattcgtgt cattaaagac cacacatgga ccacggctgt agcctgccat cagtgtgaag
    14761 atgcaccgtg cgcgaatgtc tgccctgttg acgcgataag ccgcgaacat gggcatattt
    14821 tcgttgaaca atcacgttgc attggctgta aaagctgtat gctggcttgc ccgtttggtg
    14881 cgatggaggt cgtttcttcg cgcaaaaagg cgagggcgat taagtgcgat ctgtgctggc
    14941 atcgggcgac gggaccggcc tgtgttgaag cctgcccgac aaaggcgttg cagtgcatgg
    15001 atgtcgagaa agtgcagcga catcggctac ggcagcagcc tgtttgaaac gtttttccgg
    15061 cgtttctcaa cgcagggata gtgcgctttg tttatgccgg atgcggctaa aacgccttat
    15121 ccggcctaca aattctacca aattcaatat attgcagaaa tcatgtaggc ctgataagcg
    15181 tagcgcatca ggcaattttt agtgactttc agcccaggct ctttctatct cttctgccag
    15241 aatcttcaca cccgcctcaa ttttctccgg ctccggcacg tagttcatgc gcatacattg
    15301 atgtgtatgt ggccacggtt tatccagccc tgggaagaag ttgtgccccg gcaccatcag
    15361 cacgccccgt gctttcaggc gctggtagag ctgctcggtc gtaatgggca aatccttaaa
    15421 ccatagccag aggaaaatgg ctccttccgg tttatgaatc aggcagcgat cttccggtaa
    15481 atagcggcga atgatagcga tagtttcctg aacacgctgg tagtaaaacg gtttgatgac
    15541 tgtttcagac aggcgcagca gatcgttacg cttaatcatt tcactcatca tcgccggacc
    15601 aataccgcca ggtgccaggc tgataatgcc gttcatattg gtgatggcgg tgatgatttt
    15661 ttcattggcg atgataatgc cgcagcggga gcctggtaga cccagcttgg aaagactcat
    15721 gcacagtacg atattcggat tccacagcgg gcgcgcttcg ctgaagatga tacccgggaa
    15781 cgggacgcca taagcgttat caatcaccag cggaatgccg tgttgattcg ccagcgcgtc
    15841 aagcttcagc aactcttcgt cagtaatcac attgcctgtt gggttcgtcg gccgggagac
    15901 gcaaatcatc ccggtttctt cgccaatatg cagatgctca aaatcgacgt ggtatttaaa
    15961 ctggccttcc ggcagcagct caatattcgg acgcgcagag acaaacagat cttcttccag
    16021 tccggcgtca gcgtagccaa tgtattccgg tgccagcgga aacagcactt ttttgatccg
    16081 accatcggca cggcgtccgg cgaacagatt aaataagtag aaaaacgcgc tctggctgcc
    16141 gtttgttagt gcaatattct gtggttcgat atcccaaccc aacttctcgc gtagcattcc
    16201 ggcaagcagt gtgagtagct ccgttttccc ctgtggaccg tcgtaattgc acagtgcatc
    16261 agtcgctttg ccactttcca gcatgtcggt cagtagcgtc tggaagtagt cctgcatttc
    16321 cgggatctgc gctggattac cgccgccgag cataatcgcg ccaggcgtgc gtaaaccgtc
    16381 gttcagatct tccatcagca gcgtaatgcc ggagtggcgg gtaaatttgt caccaaaaag
    16441 ggagaatgtc atagcgggat atctgtcgaa ctctgtagca aggaaggtaa caataacgct
    16501 acatttgctg gggtgcaaat cgctctgttg aagagggagc ggtgctttat cctgcaacgc
    16561 tgattaggtc tgacatttta tcctggttgg cggcttcccg aggtactgac gaacgggaag
    16621 ccggaaaagt tactgttgcc cagcccagat gaccagcact ttatcgtcgc catgctcacg
    16681 aacaaagccg tagccctgct tcagcgaaag tgtcgtttgt ttgcccgcgc caattgcggg
    16741 atggcgggcg cggaactggc tgattttctg ccagtgcgcg acgctggcgg cagatttgcc
    16801 gctaacatcc tgccagttca tatccgaacg cgtgccttgc agtggatccg aaccggtggg
    16861 gccgaacgga cgcgaggatt catcaccata aaagatttgt accgcgcctg gcgctaataa
    16921 taactctgct gctttgtcgc ccccttcacg gaacaggcgg gtatcatgcg acgagaggta
    16981 gctcaacacg ttgaaatcct gcaatttctc cgccatttgc tgccaggtcg tatccatctg
    17041 cgccagacaa tcgactgctt tagccgcctg ctcctgataa tcgaaattga tcatcgcatc
    17101 gaagccgtgg cgatagtagt cactttgcat cacgccgtgg ccccaggctt caccggtcat
    17161 ccagaacggt ttgtcatcta acgctttatc gggattcgct tttttccatt cgacaagcgc
    17221 ggcgctggct tcggttttca gttgctgcca ggcgggcaac tcaacatgtt tggcggtgtc
    17281 gacccgaaaa ccatcaatcc catagtcgcg aacccactga cttaaccagt gggttaagta
    17341 atcgcgaggg gtaaagccgt cgatgacttt agcgtgggta tccgttttgt ttttatagaa
    17401 caccggcaga ccagaagcgg tcgttgattc ggttttgata tccggcaaaa aggccagcga
    17461 catagtgaga tcgtcaaatc caggattgtc gtaatcgccg atatcggttc tgatccagtt
    17521 ttttccccac catttatccc agcctgtttt gtcgctgaaa ttaatgtaat cgttaaagct
    17581 atgccaggtt tgcccggcgg caggtttcca gtcgctccag cgttcaccca acgttttttt
    17641 cacttcgtca ccagaaagat ataacgcgcc aaactgatac tcctgcatat ccgccagtgt
    17701 ggcatagccg gtgtggttca tcacgacatc aaagagaata cgaataccgc gctgatgcgc
    17761 gctatcaacc agtgtccgta gatcggcttc actgcccata ttggcatcaa gattcgtcca
    17821 gtcctgtgtg taataaccgt ggtaggcata atgcgggaaa tcgccttttg taccgccgcc
    17881 gacccagccg tgaatttgct caaatggggc acttatccat aaagcattga cgcccagctg
    17941 ctggaggtaa tccagtttgt tggtcaggcc gcgtaaatcg ccgccgtgaa aagtgccaat
    18001 ttctgccata ccgtctttat gacgtccgta actctggtca ttactgggat cgccgttttc
    18061 gaaacgatct gtcagcacaa agtaaaccgt ggcgttatgc cagtcgaaag gggcaggggc
    18121 gtcagtttct gcccgttcca gcaggagtaa accgttgctg gtggcagcgg gttgtaacgt
    18181 tatttgaccg ttcttcacta tcgcaatttg ctggctgtaa taatcccgga cggcggctcc
    18241 ctccggaaaa gtggcgctga catccagtgt gagcggtaat ccatcccatt tcgggcattc
    18301 acggaccagg cttgctaccg gttcgacggc gttctggatg gaaatcatca atgttggtgt
    18361 accggagcgg gtgtctagtt gcagcgtata ttcgccgtcc ctgaacaatc gccattgagg
    18421 cggcgtgttg ctacaaggtt gcagggaaag catctgattg agttttatcg catccgcagg
    18481 ctgccagcac tgttggtcaa aatttagcgt tagtggacgc gtacctttgg gcaactgcgc
    18541 gtggctgaca aatgttcccg tcccctgttc gctaaaggtg ggaaaccccg gagaagtcca
    18601 gctggcggca acggcgaagc caggaaggag tgtcagaaaa caggcggcga gtttcatggc
    18661 ggatgagtcc ttattgctgc gatttcagac agtttgccag cgataagcca gacaaaactc
    18721 atccctgagc ggtaagtcca caggatgaga agcaagggtg agcgatcgcg cacaaaaaac
    18781 ggctgaattt tgcgataaac cccacatttt ctgcgattta gcgccaatct gaatcgttaa
    18841 ctcgtgacat agtttcagat ttggactatt ccttggggtg ctcttgacag ctatttttga
    18901 tatgatttga gattcaactc tcaaatttgt gaaaaatata aggtgttgga atgattaaat
    18961 ccgaccagga gacctgatga ttttgactcc catacgacga tatggggcga tgattcttat
    19021 gttactcact ctggtgtttt cgagtgaggt gttagcgaag acgcacacaa caacagcgag
    19081 tcaaaagtcc cacttaacta aagctagtaa taaacaggta agcagtaaac aagagtattc
    19141 tcgcaatagt gcaaagagta gttcacttcc tgatttgcga aaataccctt ccggaacacc
    19201 aaggaaaaag gcgtttctcc ggactgtaat gccttacatt accagccaaa atgcggccat
    19261 tactgcggaa cgtaactggc tgatttcaaa acagtatcag ggccaatggt cacctgctga
    19321 gcgtgcgcgt ctgaaagaca tcgccaaacg ctacaaggtg aagtggtctg gtaatacgcg
    19381 aaaaatcccg tggaatacct tgcttgaacg cgtagacatt atccctacca gtatggtggc
    19441 gacgatggct gcagcagaaa gcggttgggg aacgtcaaag ctggcgcgca acaacaacaa
    19501 tctgttcggc atgaaatgca tgaaaggacg ttgtaccaat gcgccgggta aagtgaaagg
    19561 gtactcacag tttagttctg tcaaagaatc ggtgagcgcc tatgtcacta acctgaatac
    19621 tcatccggct tactcttcgt tccgtaaatc gcgtgcacag ctgcgtaaag cggatcagga
    19681 agtgactgcc acagcgatga ttcacaagct gaagggctac tcgactaaag ggaagagtta
    19741 taacaactac ctgttcgcaa tgtaccagga taaccaacgg ttaatcgcgg cgcatatgta
    19801 attgcatttc ctcgagcctt atccgacttg tcagtcggat aaggcttttg cttgcatcag
    19861 gtggttgtgc tgagttacct gatgcgacgc cgacaattct catcatcgct acaacatgac
    19921 ctcgctattt acatcgcgat actcttttgg cgtcgtgtca tatgcttttt taaaaacaga
    19981 gtagaaatat tgcagcgatg gataaccgca catttgcgat atctcattga tcgacaaggt
    20041 ggttgaaatc agcagactgc gcgctttctc cagcttctcg gcatgaatca tggcatggat
    20101 ggtttcaccc acctcttctt taaaacgctt ctcaagattg gagcgcgaga tcccgaccgc
    20161 atccagtacc tgatccactt taatcccttt acaggcgtga ttacgaatgt aatgcatggc
    20221 ctgaataacg gcgggatcgg tcagcgagcg gtaatctgtt gagcgccgtt caatgacgcg
    20281 aactggcggg accaaaatcc gctgtagcgg catttcttct ttatctaata atcgatgcaa
    20341 cagttttgcc gcctgatagc ccatttgccg cgcgccctga gcgaccgaag aaagggcgac
    20401 acgcgacaga tagcgggtca gttcttcgtt atcgatgcca atcacgcata atttttccgg
    20461 tacgggaata tgtagatgtt cacatacttg cagaatatgc cgcgcccggg cgtcagtaac
    20521 ggcaataatc ccggtttgcg gtggcagcgt ttgtagccag tctgccagcc gattttgcgc
    20581 gtgttgccag ttctctggcg cggtttctaa cccctgataa accactccgc gatacttttc
    20641 ttcggcgaca agctgacgaa atgcatattc gcgttcagtg gcccaacgtt taccgcttga
    20701 ttccggaaga ccataaaaag caaagcggtt aacgcctttc tcttttaaat gcaaaaatgc
    20761 gctttcaacc agcgcatagt tatcggtggc aatgtaatga acgggtgggt aactttcggc
    20821 aaggtgatac gagccaccaa ccccaacaat ggggacgtcg acatcagcca gcgcttgctc
    20881 gatttgtttg tcgtcgaagt cggcaatgac gccatctcct aaccagtcct tgattttatc
    20941 aatgcgggcg cggaaatctt cttcaatgaa aatatcccat tccgattgtg acgcctgtaa
    21001 atattcccct acgccttcta ctacctgccg gtcataggct ttattggcat tgaacagtaa
    21061 tgtgatgcgg tgacgtttag taaacatggt tcttttcctg ctgaatcatg caaaaactca
    21121 aaaccggtaa tacgttaccg gctttgagaa aatttttatc aaaatcaaga acggcgtttg
    21181 gttgcggagt ccatccatac agccagcaac agaatcgcac ctttaacgat atactgccag
    21241 aaggtcggta catccatcat actcatgccg ttatccagtg aagccatgat gaatgccccc
    21301 attaccgctc cagccacgct cccgacgccg cctgccagac tggtgccgcc aatcacacat
    21361 gctgcaattg cgtccagctc ggcgatattc cccgcagaag gtgaaccagc gccaagtcga
    21421 gaactgagga ttaatccggc gatggctacc attaatccgt taatcgcgaa cacggcaagt
    21481 ttggtgcgtt caacgttaat cccggagaga cgtgccgctt ccagattgcc gccgatggca
    21541 taaatgcgtc gtccaaatgc tgtccgcgtt gccataaaca ttccgccgag taacagcaac
    21601 gtcagcagca gaacaggagt gggaacgcca cggtaatcat tcaacagcca gattgcgcct
    21661 aatacgatga tagcggttaa agcctggcga ccaactactg cggtagaggc gggagattgc
    21721 aaacccaaag cctgacggcg cattcttccg cgccattgcc agccaacaaa agccattaag
    21781 ccaagcgcgc caatgatgaa gccagtgctg gcaggtagat agctttgccc aatttgtgac
    21841 atcgcggcgc tggtggggga tacggtcgtg ccgttggtga tgccaatgag tatgccgcga
    21901 aatgccaaca tgcccgcgag ggtgacaata aatgaaggga ctttgcggta cgcgacccac
    21961 catccgttcc aggcaccgag aagcaatccc agaaccagcg ttacaatgat ggtaagtggc
    22021 aaaggccagc ctagccagac gtcacaaatc gccgcgacgc cacctaacag ccccatcatt
    22081 gagccgacgg aaaggtcgat ttcagcagaa attatgacga acaccattcc taccgcgagg
    22141 atgccggtaa tcgcggtttg gcgtaacagg ttggagacgt tacgggcgct taagtaggca
    22201 ccatcggtgg tccaggtaaa gaacagcatg attgcgatga tagctgcaat catcacgaag
    22261 acctgcaaat tcagtgattt cagcccggaa aagccaccgg atgtcggtac ggctaatttc
    22321 acttcagacg gattgctttt cgacatgatg ttcgctcctc aatgcggctt ccatcacctg
    22381 ctcctgagtc aggttatgat ttatcaggtt ggcttttagt ttcccttcat gcatcaccag
    22441 tacacgatcg ctaaggccga gcacttcagg taattcggaa gagatgacaa taacggcaat
    22501 accctgctgg acgagttggt taattaattt gtagatctcg tatttcgcgc caatatcgat
    22561 acccctggtg ggttcatcaa gaatgagaat gcgcgggtta agtaacagac agcgagcgag
    22621 gatcgctttt tgctgattgc cgccgctcaa acgtccaata gcaaggtcgg gggacgacgt
    22681 tttaactttg agttgctgga ttgattccag aatacatttt tgctctgccg cgtcatcaag
    22741 ctggctaatg ccaccggtaa attttttgag tgcggcgagg gtaatatttt taccaaccgc
    22801 cattaccgga acgatgccgt cgcgctttct gtcttcgggt accatcgcaa tcccctgggc
    22861 gatggcttgc tgacagttac gaatatctac ctgtttgcca tcaatataaa ttttcccttc
    22921 ccattgtccg ggccacacac caaacaggca ctgaatagtc tcggtacgtc cggcaccaac
    22981 gagtccggcg atacccagta tttcgccacg tttcagggaa aacgagacat cattaactcg
    23041 tttaatatga cgattaaccg gatgccatgc cgtcagatgt tcaatacgta atatttcatc
    23101 tccggtggta tgtggttcat tagggtaaag cgcggttaac tctcgtccga ccatcatggt
    23161 gataatatcg tcttcactca ttccagcagc atcacgcgtg ccaatgtgct gaccgtcgcg
    23221 aataacgcaa atcgtatcgg aaatcgcttt gacttcgttg agtttgtgcg aaatataaat
    23281 acaggcgata ccgtgctgtt gtagatcgcg aataatatcc agtaaaaccg acgtttcctg
    23341 ctcagttaat gaggctgtcg gctcatcgag aattaacaaa cgcacctgtt tattaagtgc
    23401 cttggcaatt tcaaccagtt gttgttgccc aagtcctaaa tcgccaacgc gggtatcagg
    23461 tgaaatggat aaactgacct gtgcgagcag cttctgacag cgtagcgtca tcagatcata
    23521 atccataatg ccattatggg ttatttcgtt acccaggaag atattttcca gcacggtcaa
    23581 ttctttcacc agggccaatt cctggtgaat gatggcgata cctttgcgtt cggtatcgcg
    23641 gatgtgactc gcctgaatct cttctcccgc aaaaataatt tcgccttcgt aggaaccatg
    23701 gggataaata ccacacagca ctttcatcag cgttgattta ccagacccat tttccccaca
    23761 gagtgagacg atttcgccag cattcaaccg caagctgacg ttatcaatcg ccttcacact
    23821 gccgaaggtt ttggtaatgt tcttcatttc aagtagataa ggcataacga ctccacctaa
    23881 gccaattaat tcacacggca tggagagaaa tcacgccccc gctcctcgcc ggggcgtaac
    23941 gcttacagct cgctctcttt gtggaatccg tctttaacta ccgtgtcttt gatgttgttt
    24001 ttattcacat cgatcggtgt cagcaggcgg gagggaacat ctttcaggcc attattcagt
    24061 gtggtatctg cttttggttc ctgaccattg cccaactcaa cggcaatttc tgcggcagta
    24121 tttgccaaca acgtaatagg tttatacacc gtcatagttt gcgtaccggc agcaatacgt
    24181 ttaatacctg cgagatccgc atcctggccg gaaattgcta ctttccctga taaaccttgc
    24241 gcgcttaatg cctgaattgc cccacctgcg gtggcatcgt ttgaggcaac tacagcatca
    24301 attttgttat tattggcggt tagcgcgttt tccataattt tcaatgcgtt ttccggtaac
    24361 cagccatcaa cccattggtc accaacgact ttaatttttc cagaatcaac gtaaggtttt
    24421 aacactttca tttgtccggc gcggaacagc ttagcgttgt tatctaccgg cgagccgccc
    24481 atcaggaagt aattaccttg cggaacaata tcgaccaggg cttttgcctg cagttcaccg
    24541 actttttcgt tatcgaaaga aatataaaaa tcgatatccg catcgttaat catacggtcg
    24601 taagctaata ctttaatacc ttcttgtttg gcttctttta caacgttact taatacctga
    24661 ccgttataag gaataatgac aagaacatcg acaccccggt taatcatgtt ttcaatctgc
    24721 gacatttgtg tttcttcatt gccatttgca gactgtacaa atactttcgc gccgagagat
    24781 tctgcttttt tcacaaaaat atctcgatct ttttgccagc gttcaagacg gagatcatca
    24841 atcgccatac ctattttgac ttctttggcg tgtgccgcaa cgttggtaag cagaagtgag
    24901 gtgcaaaggg tgagtagaat gttctttatt ttcatggtgt agggccttct gtagttagag
    24961 gacagcttta ataagtaaca atcaccgcga taaacgtaac caatttttag caactaaaca
    25021 ggggaaaaca attacagatt tttatctttc gattacgatt tttggtttat ttcttgattt
    25081 atgaccgaga tcttactttt gttgcgcaat tgtacttatt gcatttttct cttcgaggaa
    25141 ttacccagtt tcatcatccc attttatttt gcgagcgagc gcacacttgt gaattatctc
    25201 aatagcagtg tgaaataaca taattgagca actgaaaggg agtgcccaat attacgacat
    25261 catccatcac ccgcggcatt acctgattat ggagttcaat atgcaagcct attttgacca
    25321 gctcgatcgc gttcgttatg aaggctcaaa atcctcaaac ccgttagcat tccgtcacta
    25381 caatcccgac gaactggtgt tgggcaagcg tatggaagag cacttgcgtt ttgccgcctg
    25441 ctactggcac accttctgct ggaacggggc ggatatgttt ggtgtgggag cgtttaatcg
    25501 tccgtggcag cagcctggtg aggcactggc gttggcgaag cgtaaagcag atgtcgcatt
    25561 tgagtttttc cacaagttac atgtgccatt ttattgcttc cacgatgtgg atgtttcccc
    25621 tgagggcgcg tcgttaaaag agtacatcaa taattttgcg caaatggttg atgtcctggc
    25681 aggcaagcaa gaagagagcg gcgtgaagct gctgtgggga actgctaact gctttacaaa
    25741 ccctcgctat ggcgcgggtg cggcgacgaa cccagatcct gaagtcttca gttgggcggc
    25801 aacgcaagtt gttacagcga tggaagcaac ccataaattg ggcggtgaaa actatgtcct
    25861 gtggggtggt cgtgaaggtt acgaaacgct gttaaatacc gacttgcgcc aggagcgtga
    25921 acagctgggc cgctttatgc agatggtggt tgagcataaa cataaaatcg gtttccaggg
    25981 cacgttgctt atcgaaccga aaccgcaaga accgaccaaa catcaatatg attacgatgc
    26041 tgcgacggtc tatggcttcc tgaaacagtt tggtctggaa aaagagatta aactgaatat
    26101 tgaagctaac cacgcgacgc tggcaggtca ctctttccat catgaaatag ccaccgccat
    26161 tgcgcttggc ctgttcggtt ctgtcgacgc caaccgtggc gatgcacaac tgggctggga
    26221 caccgaccag ttcccgaaca gtgtggaaga gaatgcgctg gtgatgtatg aaattctcaa
    26281 agcaggcggt ttcaccactg gcggtctgaa cttcgatgcc aaagtacgtc gtcaaagtac
    26341 tgataaatat gatctgtttt acggtcatat cggcgcgatg gatacgatgg cgttggcgct
    26401 gaaaattgca gcacgcatga ttgaagatgg cgagctggat aaacgcatcg cgcagcgtta
    26461 ttccggctgg aatagcgaat taggccagca aatcctgaaa ggccaaatgt cactggcaga
    26521 tttagccaaa tatgctcagg aacacaattt gtctccggtg catcagagtg gtcgccagga
    26581 gcaactggaa aatctggtaa atcattatct gttcgacaaa taacggctaa ctgtgcagtc
    26641 cgttggcccg gttatcggta gcgataccgg gcattttttt aaggaacgat cgatatgtat
    26701 atcgggatag atcttggcac ctcgggcgta aaagttattt tgctcaacga gcagggtgag
    26761 gtggttgctt cgcaaacgga aaagctgacc gtttcgcgcc cgcatccact ctggtcggaa
    26821 caagacccgg aacagtggtg gcaggcaact gaccgcgcaa tgaaagctct gggcgatcag
    26881 cattctctgc aggacgttaa agcattgggt attgccggcc agatgcacgg tgcaacctta
    26941 ctggatgccc agcaacgggt attacgccct gccattttgt ggaacgacgg gcgctgtgcg
    27001 caagagtgca ctttgctgga agcgcgagtt ccgcaatcac gggtgattac cggtaacctg
    27061 atgatgcccg gatttactgc gcctaaattg ctatgggttc agcggcatga gccggagata
    27121 ttccgtcaaa tcgacaaagt attattaccg aaagattact tgcgtctgcg tatgacgggg
    27181 gagtttgcca gcgatatgtc tgacgcagct ggcaccatgt ggctggatgt cgcaaagcgt
    27241 gactggagtg acgacatgct gcaggcttgc cacttatctc gtgaccagat gcccgcatta
    27301 tacgaaggca gcgaaattac tggtgctttg ctacctgaag ttgcgaaagc gtggagtatg
    27361 gcggcggtgc cagttgtcgc aggcggtggt gataatgcag ctggtgcggt tggtgtagga
    27421 atggttgatg ctaatcaggc aatgttatcg ctggggacgt cgggggtcta ttttgctgtc
    27481 agcgaagggt ttttaagcaa gccagaaagc gccgtacaca gcttttgcca tgcgctaccg
    27541 cagcgttggc atttaatgtc tgtaatgctg agtgcagcgt cgtgtctgga ttgggccgcg
    27601 aaattaaccg gcctgagcaa tgtcccagct ttaatcgctg cagctcaaca ggctgatgaa
    27661 agtgccgagc cagtttggtt tctgccttat ctttccggcg agcgtacgcc acacaataat
    27721 ccccaggcga agggggtttt ctttggtttg actcatcaac atggccccaa tgaactggcg
    27781 cgagcagtgc tggaaggcgt gggttatgcg ctggcagatg gcatggatgt cgtgcatgcc
    27841 tgcggcatta aaccgcaaag tgttacgttg attggtggcg gggcgcgtag tgagtactgg
    27901 cgtcaaatgc tggcggatat cagcggtcag cagctcgatt accgtacggg gggggatgtg
    27961 gggccagcac tgggcgcagc aaggctggcg cagattgcgg cgaatccaga gaaatcgctc
    28021 attgagttgt tgccgcagtt accgttagaa cagtcgcatc taccagatgc gcagcgttat
    28081 gccgcttatc agccacgacg agaaacgttc cgtcgcctct atcagcaact tctgccatta
    28141 atggcgtaaa cgttatcctc tgcctgaccg ggtgggggat aattcacatc tatatatctc
    28201 agtaattaat taatatttag tatgaattta ttctgaaaat catttgttaa tggcattttt
    28261 cagttttgtc tttcgttggt tactcgtaat gtatcgctgg tagatatgga gatcgttatg
    28321 aaaacctcaa agactgtggc aaaactatta tttgttgtcg gggcgctggt ttatctggtt
    28381 gggctatgga tctcatgccc attgttaagt ggaaaaggct attttcttgg catattaatg
    28441 acagcaactt ttggcaacta tgcatatctt cgcgcagaaa aactcgggca actggataat
    28501 ttttttaccc atatctgcca gttagttgcg ttaatcacta tcggtctctt gtttatcggt
    28561 gttttaaacg cacctatcaa tgcttatgaa atggtgatct accctatcgc cttttttgtc
    28621 tgcttgtttg gtcaaatgcg tttgtttcgc tcggcatgag caacataaag ctcttacaca
    28681 ttcaggaatg aaggaatact gtgatggaca acaaaatatc aacctattct ccggcattca
    28741 gtattgtgtc atggatagct ctcgttggtg gtatcgttac ctatctgtta gggctatgga
    28801 atgcagagat gcagttaaat gaaaaaggat attattttgc cgtactggta ttaggactgt
    28861 tttctgcggc gtcttatcaa aagaccgttc gggacaagta tgaaggcata ccgaccacct
    28921 ccatttatta tatgacctgc ctgactgtct ttattatctc tgttgcgtta ctgatggtag
    28981 gtctgtggaa tgcaacgtta ttactcagcg agaaaggttt ttatggactg gctttcttct
    29041 taagcttgtt tggtgcagta gcggtgcaga agaatattcg tgatgccgga ataaacccac
    29101 caaaagaaac acaggttacc caggaagaat acagcgaata attcacataa gcccggtcag
    29161 tccaatgtga ccggcctttt acttaactca ctagtctgtt tctgtcgatt cgttgtacca
    29221 gcatagaaag taacaaactc gctgccaacg tcgcgcaaaa gatccaaata atatcgagta
    29281 ttggccagtt tttaagctca atcccccggg tgcgcagtgc atggataatc aaggcgtgga
    29341 acccgtatat gcccaacgaa tggcgggaga ttaagccaag tccgcgaacg gtacgcgtat
    29401 caagcgtgtt tttaaccaga gtcaatagcg tgattgcgca gataaaaacc atcggcccac
    29461 agtaaagata ccaggtatcg gcaaaatttc cgcgccactg caattcatat aatgtcccgc
    29521 gagagataat aaaaaccccc gtcgcaaaca gtgctgcgca cacccacgac agtgctttat
    29581 gctgtgtgtc catcatccct atagcgcggc ccaacatgcc atacaggatg tagtaaaaag
    29641 tatcgccatt gatatataag ttaattggca gccattcaaa accgtcaatt ttctgcggca
    29701 ctgtgtttgg gttagcgata ataccaatca ccaccattag caccagcaac atttttccgc
    29761 cgacgttctt cacctgaatc agcggtgaaa ccagataaat caccgcaatc gcgaagaaaa
    29821 accataagtg gtaaaacact ggcttttgca gcaggttttt cagcgctaac tccacattga
    29881 tggaggtaaa cagcgcaatg tagagcagtg cgattgcgct ataaaaaagc agacataagc
    29941 cgatacgcaa gaaatggcgc ggctgggcgc tgcgttcgcc aaaaaagaga tagccggaaa
    30001 tcatgaaaaa tagcggcacg ctgacacgag aggcagaatt cagaacattg gcgatatccc
    30061 aggtgacggg gctaacacta tgagcattgg tcacatacca ggtagtggtg tgaatcatca
    30121 ccaccattaa acacgctatc cctcgcaggt tatcaatcca gtaaattttg ggctgcatct
    30181 gtgtctctgt atctggttaa aaaagtctga ccgataaatc attcggttgg cgcactggaa
    30241 taatctgagt tttatcacta cagcttatag aggcttaagg aaattcgtaa gatatcagcc
    30301 actataccga tataaataat aagactcacc tgcaaaccag acggtaattt aatgatgatg
    30361 aactctttct ttccggcaat ggcgcttatg gtgcttatgg cgctagtggg ttgttctaca
    30421 ccgccacccg tgcagaaagc acaacgggta aaggttgatc ctctgcgttc gttgaatatg
    30481 gaagcgttat gcaaggatca ggcggcgaaa cgttataaca ccggcgagca aaaaatcgac
    30541 gtcaccgcat ttgaacagtt ccagggaagc tacgaaatgc gcggttatac cttccgtaaa
    30601 gagcagtttg tctgttcttt tgacgcagat ggccattttt tgcatctttc catgcgttaa
    30661 gccctgcttt ttcccgtttc gtactgtata tcttccatcc agcgggtata ctgatccctt
    30721 cctttaaatc cacacgtatc cagcacgaaa taatatgcaa aagtttgata ccaggacctt
    30781 ccagggcttg atcctgacct tacaggatta ctgggctcgc cagggctgca ccattgttca
    30841 accattggac atggaagtcg gcgcgggaac ctctcaccca atgacctgtc tgcgcgcgct
    30901 ggggccagaa ccgatggcgg ctgcttatgt tcagccttct cgtcgcccga ccgatggtcg
    30961 ctacggcgaa aaccccaacc gtttacagca ctactatcag ttccaggtgg tcattaagcc
    31021 atcgccggac aatattcagg agctgtacct tggttctctg aaagagctgg gcatggaccc
    31081 gaccatccac gacatccgtt tcgtggaaga taactgggaa aacccgacgc tgggtgcctg
    31141 ggggctgggc tgggaagtgt ggctgaacgg catggaagtg acgcagttca cttacttcca
    31201 gcaggttggt ggtctggagt gtaaaccggt taccggcgag atcacctacg gtctggaacg
    31261 tctggcaatg tacattcagg gcgtagacag cgtttacgac ctggtctgga gcgacggccc
    31321 gctgggtaaa accacctatg gcgacgtgtt ccatcagaac gaagtggagc agtccactta
    31381 caacttcgaa tacgcggatg tggacttcct gttcacctgc tttgagcagt acgagaaaga
    31441 agcgcagcag ctgctggcgc tggaaaatcc gctgccgctg ccagcctacg agcgtattct
    31501 gaaagccgcc cacagcttca acctgctgga tgcgcgtaaa gccatctccg tcaccgagcg
    31561 tcagcgctat attctgcgca ttcgcaccct gaccaaagca gtggcagaag catactacgc
    31621 ttcccgtgaa gccctcggct tcccgatgtg caacaaagat aagtaagagg cggctatgtc
    31681 tgagaaaact tttctggtgg aaatcggcac tgaagagctg ccaccaaaag cactgcgcag
    31741 cctggctgag tcctttgctg cgaactttac tgcggagctg gataacgctg gcctcgcaca
    31801 cggcaccgtt caatggtttg ctgctccgcg tcgtctggcg cttaaagtag ctaacctggc
    31861 ggaagcgcaa ccggatcgtg aaatcgaaaa acgcggcccg gcgattgccc aggcgttcga
    31921 cgctgaaggc aaaccgagca aagcggcaga aggctgggcg cgtggttgcg gtattaccgt
    31981 tgaccaggct gagcgtctga ctaccgataa aggcgaatgg ctgctgtatc gcgcccacgt
    32041 gaagggcgaa agcactgaag cactgctgcc gaatatggtt gcgacttcgc tggcgaaact
    32101 gccgatcccg aaactgatgc gttggggtgc aagcgacgtg cacttcgtgc gtccggtgca
    32161 caccgtgacc ctgctgctgg gcgacaaagt tattccggct accattctgg gcattcagtc
    32221 cgatcgcgtg attcgcggcc accgctttat gggcgagccg gaattcacta tcgacaatgc
    32281 tgatcagtat ccggaaattc tgcgtgagcg cgggaaagtc atcgccgatt acgaagaacg
    32341 taaggcgaag attaaagccg atgccgaaga agcggcgcgt aagattggcg gtaacgctga
    32401 cttaagcgaa agcctgttgg aagaagtcgc ttcgctggtg gaatggccgg ttgtgctgac
    32461 cgcaaaattc gaagagaaat tcctcgcggt gccgtctgaa gcgctggttt acaccatgaa
    32521 aggtgaccag aaatacttcc cggtgtatgc gaacgacggc aaactgctgc cgaactttat
    32581 cttcgttgcc aacatcgaat cgaaagatcc gcagcaaatt atctctggta acgagaaagt
    32641 cgttcgtcca cgtctggcgg atgccgagtt cttcttcaac accgaccgta aaaaacgtct
    32701 ggaagataac ctgccgcgcc tgcaaaccgt gctgttccag caacagctgg gtacgctgcg
    32761 tgacaagact gaccgcatcc aggcgctggc tggctggatt gctgaacaga ttggcgctga
    32821 cgttaaccac gctacccgtg cgggcctgct gtccaagtgc gacctgatga ccaacatggt
    32881 cttcgagttc accgacaccc agggcgttat ggggatgcac tacgcgcgtc acgatggcga
    32941 agcggaagat gttgccgtgg cgctgaacga gcagtatcag ccgcgctttg ccggtgatga
    33001 cctgccgtct aacccggtag cctgtgcgct ggcgattgct gacaagatgg atactctggc
    33061 gggtatcttc ggtatcggcc agcatccgaa aggcgacaaa gacccgtttg cgttgcgtcg
    33121 tgccgcactt ggcgtgctgc gtattatcgt tgagaagaac ctcaaccttg atctgcaaac
    33181 gctgaccgaa gaagcggtgc gtctgtatgg cgataagctg actaatacca acgtagttga
    33241 tgatgttatc gactttatgc tcggtcgctt ccgcgcctgg tatcaggacg aaggttacac
    33301 cgttgatacc atccaggcgg tactggcgcg tcgtccgact cgtccggcag atttcgatgc
    33361 ccgtatgaag gcggtatcgc acttccgtac tctggatgcg gcagcgtcac tggcggcggc
    33421 aaacaagcgt gtatctaaca ttctggcgaa gtctgacgaa gtgctgagcg atcgcgtgaa
    33481 tgcttctact ctgaaagagc cggaagaaat taaactggcg atgcaagttg tggtgctgcg
    33541 cgacaagctg gagccgtact tcgctgaagg tcgttaccag gacgcgctgg tcgaactggc
    33601 tgaattgcgt gaaccggttg atgccttctt cgataaagtg atggtcatgg ttgatgacaa
    33661 agaattgcga atcaaccgtc ttaccatgct ggagaaactg cgcgaattat tcctgcgcgt
    33721 tgcggatatt tcgctgttac agtaataacg ccgttattaa aaagcctgcc atctggcagg
    33781 cttttttatc gctaaataat agagcaacct ttaataatct tctgctgaat aaagattgtc
    33841 tcatatatta attttatgag atttttttag gattatatca aggagaagaa acgaacttat
    33901 taagctagaa tagctgtggg tgcttgagac tgtttgtctc aggtattcac cgaaaggcag
    33961 acagagaaaa gccccacctg actataaatc aaagtgaggc taccctatgc ctgaacacca
    34021 taaggttagc ctcttactcg ttggaaatca acacaggagg ctgggaatgc cgcagaaata
    34081 tagattgctt tctttaatag tgatttgttt cacgctttta ttttttacct ggatggtaag
    34141 agattcactg tgtgaattgc atattaaaca gggaagttat gagctggcgg catttttagc
    34201 ctgcaatttg aaagagtaag agactttggc gggaagttat tcccgccttt cttacggcgt
    34261 tgcgcattct cattgcaccc aaatttattc ttcacaaaaa taacactaga ttctattacg
    34321 cgatcgatta tttatttcct gaaaaacaaa taaaaaaatc cccgccaaat ggcagggatc
    34381 ttagattctg tgcttttaag cagagattac aggctggtta cgttaccagc tgccgggcct
    34441 ttagcgccgc tttcgatggt gaaggacact ttctgacctt cgtccagaga tttgtaacca
    34501 tcgttctgga tagcagagaa gtgtacgaac acgtctttag agccatcgtc aggagtgatg
    34561 aagccgaagc ctttgtcagc gttgaaccat tttacgatac cagtcatttt accggacata
    34621 gtgtattacc tttaataatt aagtgtgcct ttcggcgata tggcgtgctt tacagatttt
    34681 gaagcgttaa aggaatgtgc actacgaggg gtatcaacga taactcttga agggacttgc
    34741 cttactacac tgctttaatg gtctgtacgt caaaccgttg atgtgcatta agccacgcat
    34801 tggcgggtga tgcaacaatt atttttcata tttatgatta atcggttgga aaacggtcct
    34861 gtcatcagga ccgtaaacag caataaagtg gataaaagtc tattccatca actgcttact
    34921 taatgccggg ttggcttgaa tcaaacgcat caattttagt tcggcacttg aaggcttcac
    34981 gcgtctggat tcccattcct ttaccatggc gactgatacg cccaaaaccc gggcgaaatc
    35041 atcgattttt aaccctgtcc cttttcgtaa ctgctcaatt tcggtacagg acgttgttct
    35101 gtgcgtcagg gtaattttct gcgtttcatc tttaaaaaca atctgttcca ggctgctcaa
    35161 cagctcatgc attggatctt tatattccat tgaaaactcc tcaaatcaca ctgcgggatc
    35221 gtgaattgca tcgaagctca ttaagaatag tcgggaaaaa cagacatagc ggggtgtagt
    35281 cagggcatgc tctggctatg attcgattca gccagtcatt tagcgtgact tatagccacc
    35341 taacgatctg ataaatgccg atatatctct taccactccc ggtttgtaaa gatttcgaaa
    35401 atctaacgtt ctgcgaaggc gtaaatctca ggctacaaaa aaagcaacgg cactatcatt
    35461 ttttaacaat gaaacaacaa attgaccttg tttcattttc tccatgattt taccaggaag
    35521 attacgtaga aagggtatcc tgcaagcctg tcctggacta tccttgtcac gtcagacacg
    35581 cgtgtgtcgt tgtgcgcttt ttttgggtga aaggagtaag aaaatggcga caggaaagtc
    35641 ctgctctcgc tggtttgcgc ctcttgcggc gttattaatg gtagttagcc tgagtgggtg
    35701 ttttgataaa gaaggcgatc agcgtaaagc gtttatcgac ttcctgcaga atacagtgat
    35761 gcgtagcggt gaacgtctac caaccctgac tgccgatcag aaaaaacagt ttggtccttt
    35821 tgtctctgat tacgcgattc tgtatggtta ttctcagcag gtgaatcagg cgatggattc
    35881 cggtctgcgc ccggttgtag acagcgttaa cgcaattcgc gtaccgcagg attacgttac
    35941 gcaaagcggc ccactgcgtg aaatgaacgg ttctctgggc gtactggcgc aacagctgca
    36001 aaatgcgaag ttgcaggccg atgctgcgca ctctgcgcta aaacagagcg atgacctgaa
    36061 accggtcttc gatcaggcat tcaccaaagt ggtaacgacg ccagctgatg cgttgcaacc
    36121 gttgattccg gcggcacaaa ctttcacgca acaactggtg atggttggag actacattgc
    36181 tcagcaaggt actcaggtga gctttgtcgc aaatggcatc cagttcccga cttcgcagca
    36241 ggcaagtgaa tataacaaac tgattgcgcc attaccggca caacatcagg cgtttaatca
    36301 ggcctggact acagcagtta ctgcaaccca ataaagagta aaagcccgag cgattatcgg
    36361 gctttactcc acgcagtcgc ggcttagtcc gcgacgtgcg gattcacaca gttcttctca
    36421 acctttcctt gtaacgcatc aatcaagtta tccacggcac aggcggccat gccataacgc
    36481 gtctcatgag tggcagatcc aatatgcggt actgcgacga cgttggccat tgagagcaac
    36541 ggcgaatcta ctgacagtgg ctcttgttcg aagacatcca gcccggcagc gtgaatttcc
    36601 cctttctgca atgcagcaat cagtgcattt tcatcaacca ccgggccacg tccggcatta
    36661 atgaaaatgg cggaggattt cattttggcg aattgttctg cgccaaacag atgatgcgtt
    36721 tcatcagtta acggcaggat caggcaaacg aaatctgact cttgtaacag tgtatccaaa
    36781 tcgcagtagc gggcgttgaa gcgttcttct gcttctttat ggtggcggcg cgcgttatag
    36841 aggatgggca tgttgaagcc aaagtgcgca cgttgtgcca gcgccatgcc gatccgtccc
    36901 atcccgacaa tgcccagtgt tttatggtga acgtcagtgc cgtaccagtc cgggcctatg
    36961 ctcgcggtcc attcgcctgc ttttactcgt tctgctacct ccacaacccg acgagcggta
    37021 gacaatacca gcgccatcag cgtatcggcg acggtttctg ttaatacggt tggcgtgtgc
    37081 atcagcagaa tttttcgggc ggtaagcgca tcgacatcaa aattgtcata gcctacggag
    37141 atcgttgatg tggcacgcag tttcggcatt ttttccagca atgcggcatc aacattctcg
    37201 tttgaaccca gtaaaccttc agcttcggca agaattgctg cattttgttc gatggtttgt
    37261 gggctgaggt ttgccacctg gtgaacggtg aaatgctctt gcaggcgttg cagtaaatca
    37321 tcaggtaagg ctttgtagag gataacggac ggcttcattc ttctctccat tcactgataa
    37381 ggcgatcacc ttagcctgaa atatgatgaa ggaaaactga agaatgccag gtgatttctg
    37441 catcacctgg catgaaaggg attacagcgg gcttaaggta atttctacac ggcggttttg
    37501 cgccttacct tctgcagtgc tgttgctggc gattgggtta gccgggccaa ggccctgagt
    37561 acggatgcgg ctggcgtcca cgccctgggt gatcaacgcg ctggcaacgg aatccgcacg
    37621 ctgctgggag agacgcatgt tcaggtcgtg cccacccgtg ctgtcggtat aaccaatcac
    37681 gttaaccgcc gttttcggat actctttcag taccattgcc acgccggtca gggtgttagc
    37741 gcccgccggt ttcagggttg cgctgctgct gtcgaaggtc acattgttcg gcatattgag
    37801 gataatgtta tccccgctgc gggttacgct aacaccagtg ccgcgcattt tgtcacgcag
    37861 cttcgcttcc tgcacatcca tgtaataacc cacgccgccg cccagagctg cgcctgctgc
    37921 tgcgccaatg agcgcgcctt taccgcgatc tttcttcgaa gaagagagcg caccaatacc
    37981 cgcgccaacg agagagccca gacctgcgcc gatagcagat ttacctgctt cgcgttcgcc
    38041 ggtgtaaggg ttagttgtac agccagatac cgccagagcg ccactcacta cggcggcaat
    38101 aagataaaca cgtttcttca ttgttaatcc ttaataacct ttttattctt tgccacgggt
    38161 tctgtggcgg gagattatgc cgcgtgaaca tgaagattat tcctgggaat actcggaaat
    38221 ttgtaagtaa tatttaactg ctcaatacat ctaacctttc aggagtcttc ggtttggcca
    38281 actcatcctc acgatattcc gttctgactg ccgcccactg ggggcctatg ctggttgaaa
    38341 ccgacggcga aaccgtgttt agctcgcgtg gcgcgttagc cacaggaatg gaaaactcct
    38401 tgcagagcgc ggttcgcgac caggttcaca gcaatacgcg ggtgcgattt ccaatggtgc
    38461 gcaaaggctt tcttgcgtca ccggaaaatc cgcaaggcat tcgtgggcag gatgaatttg
    38521 ttcgcgtgag ttgggatgag gcgctggagc ttattcacca tcaacataaa cgcattcgtg
    38581 aggcttatgg tccggcatcg atttttgctg gttcctacgg ctggcgttca aacggcgtgc
    38641 tgcataaggc ctcgacatta ttacaacgct atatggcgct ggcaggcggt tatactgggc
    38701 atctggggga ttattcgacc ggcgcggcac aggcgatcat gccgtatgtc gtgggtggca
    38761 gcgaagttta tcaacagcaa accagttggc cgctggtgct ggaacatagc gatgtcatgg
    38821 tgctgtggag tgctaaccca ctcaatacgc tgaaaattgc gtggaatgca tccgatgagc
    38881 aggggctttc ttacttttcc gcactgcgtg acagcgggaa aaagctgatc tgcattgatc
    38941 caatgcgatc ggaaaccatc gatttctttg gcgataaaat ggagtgggtg gcaccacaca
    39001 tgggcaccga tgttgcgttg atgctgggga tcgcccatac gctggtggaa aatggttggc
    39061 acgacgaagc gtttctggcg cgttgcacca caggttatgc cgtcttcgcc tcttatttgc
    39121 tgggcgagag tgatggtata gcgaaaaacg ccgaatgggc ggcagagatt tgtggtgttg
    39181 gcgcagcgaa aatccgcgag ctggcggcta ttttccacca aaatacaacc atgctgatgg
    39241 caggctgggg aatgcagcgc caacagtttg gtgagcaaaa acactggatg ctcgtcacgc
    39301 tggcggcaat gttggggcaa atcggcacac ccggcggcgg ttttggtctt tcttaccatt
    39361 ttgccaatgg tggtaacccc acgcgccgcg ctgcggtgct ctcttccatg cagggcagtt
    39421 tgcctggcgg caccgatgcg gtggataaaa tccctgttgc ccgcattgtt gaagcactgg
    39481 aaaaccccgg tggcgcatat caacataacg gtatggaccg acatttcccg gatattcgtt
    39541 ttatctggtg ggcgggtggt gccaacttta ctcatcatca ggataccaat cgcctgatcc
    39601 gtgcctggca aaaaccggag ctggtggtga tctctgaatg cttctggacg gcggcggcga
    39661 aacacgcgga tatcgttctg cctgcgacca cctcttttga gcgtaatgat ctcaccatga
    39721 ccggcgatta cagtaatcag catctggtgc cgatgaagca agtggtgccg ccacgctatg
    39781 aagcgcgtaa tgattttgat gtttttgccg agttaagtga acgttgggag aagggcggct
    39841 atgcgcggtt tacggaagga aaaagtgagc tgcaatggct ggaaacgttt tataacgttg
    39901 cccggcaacg cggggcaagc cagcaggttg aattgccgcc atttgctgag ttctggcaag
    39961 ccaaccagtt aattgagatg ccggaaaacc cggacagcga gcggtttatt cgcttcgccg
    40021 attttcgccg cgatccgcag gcgcatccat taaaaaccgc cagcggcaag attgaaatct
    40081 tctcgcagcg tattgccgat tacgcttatc cggattgccc tgggcatcca atgtggctgg
    40141 agccggacga atggcagggc aatgccgaac cggaacagtt gcaggtactt tctgctcatc
    40201 cggcacatcg tctgcacagc cagctgaatt acagttctct gcgcgaattg tacgcggtgg
    40261 caaaccgtga gcctgtcacc attcatcctg acgatgctca ggcgcgcggc atacaagatg
    40321 gcgatactgt tcggctgtgg aacgcacgcg ggcaaattct tgccggggca gtcattagcg
    40381 agggaattaa acctggcgtg atttgcattc acgaaggggc atggccggat ctggatttaa
    40441 ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct gaccaaagat ctccccagct
    40501 cgcggctggg gaatggctgt gcgggtaata cggcgctggc atggctggaa aaatacaacg
    40561 gtccggaact gacacttaca gcgtttgaac caccggccag ctcataatcc aggtgggtag
    40621 ttgggtttca tcctgccatg cgcagtcgac aatgtgaaaa ccctgcgcct ggtaaaaatc
    40681 tatcgccggt tgattttttt gataaacctc cagcatcaga tggggatggc gctgctgcac
    40741 atactgcatt agcgccttac caataccgcg tctgacggcc ttcggtgcga caaacattgc
    40801 agccagaaat cggccttcca taatgctgac aaaaccgaga agcttaccgt cttcttccca
    40861 gacccagttt tgcgcgttgg caagatacgc atcgcgcacc agcggaatgc agtcacgcca
    40921 gtaactcgat tttataaagg gatgccccca ggttgtactt tccagccaca gttcgaggat
    40981 cgcggggagt tctgaatgtt gcacttcccg aatcatggtt tatttcccgg atggcaacag
    41041 cagccaacca catgatcatt caccagccca catgcctgca taaaggagta acagattgtg
    41101 gtaccgacaa acttaaaacc acgttttttt agtgccttag atagggcgtc ggaggcgggt
    41161 gtagatgtgg ggatttcgct caacgttgtg gcttgtgtca cctgtggttg atgattgaca
    41221 aacgaccaga caaagtcggc aaacggttcg ccgttctgtt ccatttgcag gtacgcccgc
    41281 gcattaccaa taattgcctg aattttcccg cgatggcgga taatcccggc gtcctgtacc
    41341 agtctttcga catcctcttc ctgcattgca gccaccttca ccggatcgaa ctgatgaaag
    41401 ctggcgcgat agttttcgcg ttttttgagg acggtgatcc acgataatcc agcctgctgc
    41461 ccttcaaggc agatcatttc gaacagtttt ttactgtcag tttcaggcac gccccactca
    41521 ttatcatggt aggcaatata aagcgggtcc tgactcaccc agccgcaacg ttccatactt
    41581 tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt ttcttgacgc atcggtgaaa
    41641 aagtcaatat ttaatacagg gctactatgc ccgatatcct tcatacaatg acaataatat
    41701 tgccgaattc ggctcttccc tggagtcgtg ttaatgatca taaaaaaaag cggtggtcgc
    41761 tggcagctaa gcctgctggc gagtgtggta atcagtgcct tttttctcaa cacagcttac
    41821 gcctggcaac aagaatatat cgttgatacg caacccggac attccacaga gcgttacacc
    41881 tgggatagcg atcatcaacc tgattacaac gatattttgt cgcaacgtat tcaaagtagc
    41941 caaagggcgc tgggactgga agtcaatctg gcggaagaaa ctcctgtgga tgtgaccagc
    42001 agtatgagta tgggctggaa ttttccttta tatgagcagg ttacaaccgg cccggtcgcg
    42061 gcattacatt acgatggcac aaccacctcg atgtataacg agtttggcga cagtactacc
    42121 acgctggccg atccgttatg gcatgccagc gtgagtacct taggctggcg tgttgactcc
    42181 cggcttggcg atctccgacc ctgggcgcaa atcagctata accagcaatt tggcgagaat
    42241 atctggaagg cgcaatcagg cctgagccgg atgacggcga caaaccagaa cggcaactgg
    42301 ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa atattgctgc ctatgctgcg
    42361 ctatctcagg cagaaaatac cactaacaat agcgactatc tgtatacgat gggggttagc
    42421 gccagatttt aacgttatag atacaattga aaccaataaa taacaactaa tgtggcgata
    42481 gtgggtgata acttactaaa taataaattt ggtgaataat tgtcgagtca ttcattcctg
    42541 aactaatgca tttcattccg ttctgatggc atttcatgcc gtttttcccc aggcataaag
    42601 tgcacttcgt tatggttgtc agcagagatt tttccttttt attactgcag gaatactgcc
    42661 atgacacctt caaattatca gcgtacccgc tggttgacac tcatcggtac tatcattacc
    42721 cagtttgccc ttgggtcggt ttatacctgg agcctgttta acggcgcgct ttctgccaag
    42781 ctggatgcgc cggtaagcca ggtcgctttc tctttcggct tgttaagtct ggggctggca
    42841 atttcatctt ctgttgcggg gaaattacag gaacgttttg gcgttaaacg cgtcactatg
    42901 gcttccggca ttttgctggg attaggcttc ttcctgacag cgcattctga caacctgatg
    42961 atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg atggcgcggg ttacctgctg
    43021 acgctctcta actgcgtgaa gtggttcccg gagcgtaaag gtctgatctc cgcgttcgct
    43081 atcggttctt atggtctggg tagcctgggt ttcaaattta tcgacacgca gctgctggaa
    43141 acggtgggtc tggaaaaaac ctttgtgatt tggggagcga ttgcgctggt gatgattgtc
    43201 tttggcgcaa cattaatgaa agacgcacca aaacaggaag tgaaaaccag caatggtgtg
    43261 gtggagaagg actacaccct ggcagagtcg atgcgtaaac cgcagtactg gatgttagcg
    43321 gttatgttcc tgactgcgtg catgagtggt ctgtatgtga ttggtgtagc gaaagatatc
    43381 gctcaaagtc tggcgcatct tgatgcaatt tccgcagcca atgctgtgac ggttatttcc
    43441 atcgccaacc tttctggtcg tctggtgctg ggcattctgt ctgacaaaat cgcccgtatc
    43501 cgtgttatca ccattggtca ggtgatatcg ctggtgggta tggcggccct gctgtttgca
    43561 ccattgaatg cagtgacgtt ctttgcagcg attgcctgcg tggcatttaa ctttggcggc
    43621 actatcacgg tgttcccgtc actggtcagt gagttcttcg gcctcaataa cctggcgaaa
    43681 aactacggtg tgatttatct cggtttcggt atcggcagca tttgtgggtc gattatcgcc
    43741 tcactgtttg gcggcttcta tgtgaccttc tacgtgattt tcgcactgct gattctgtca
    43801 ctggcgctct ctacgacgat tcgtcagcca gagcagaaaa tgttgcgtga ggcgcatggc
    43861 tccctttaat catctgccgc gagctactgg gctttagcct tagtgtgaat caaacgcttc
    43921 aacgcaatgt ataaacaatg acgcgtaaaa tttgggccag ttaactggcc ctttttactg
    43981 ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt tgtaaatttg tgcttcatgc
    44041 acactctttc cccacacttt ttccctttgc tgtggtctac ttattcgcgc gtgtagattt
    44101 tacttatctg actacctccg cacttttccc ctgccgggcc tgaaaagccg ctaagcaggg
    44161 tgttatcacc tgtttgtcca gggtttgttt gcatgagata catcaaatcg attacacagc
    44221 agaagctgag ctttttgctt gcaatctata ttggcctttt tatgaatggc gcggtttttt
    44281 accgccgctt cggcagctat gcgcacgatt ttaccgtctg gaaaggcatt tctgctgttg
    44341 ttgaactggc cgccaccgta ctggtgacct tctttttact acgtcttctt tcgctgtttg
    44401 gccgccgtag ctggcgtatt ctggcgtcgc tggtggtgct cttttccgca ggtgccagct
    44461 attacatgac cttccttaat gtggtcattg gttatggcat catcgcttcc gtcatgacca
    44521 ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt tattctctgg ttaatcgccg
    44581 ttagcgcgtt gcctcttatc cttatctgga ataaccgctg tcgttacacc ttgctccgac
    44641 aactgcgaac cccagggcag cgtattcgca gcctggcggt cgtcgtactg gcaggtatta
    44701 tggtttgggc accgattcgt ttgctggata tccagcagaa gaaagtggag agggcgacag
    44761 gtgttgattt gccgagttat ggcggcgtcg tcgcgaactc ttatctgcca tcaaactggc
    44821 tttcggcatt aggtctttat gcctgggcgc gggtcgatga atcttccgat aacaattctc
    44881 tccttaaccc tgcgaagaag tttacgtacc aggctccaca aaacgttgat gatacatatg
    44941 tcgttttcat tattggtgaa accacgcgct gggatcatat gggcattttc ggctatgaac
    45001 gcaataccac accgaagctg gcccaggaaa aaaatctggc ggcgttccgt ggttactcct
    45061 gtgataccgc aaccaaactc tctctgcgtt gcatgtttgt acgtcagggg ggcgcggaag
    45121 ataatccgca gcgcacatta aaagaacaga acattttcgc ggtattgaag cagttaggat
    45181 tcagttctga tctctacgct atgcaaagcg aaatgtggtt ctacagcaat acgatggcgg
    45241 acaacattgc ttatcgtgag cagattggtg cggagccacg taaccgtggc aaaccggtag
    45301 atgatatgtt gctggtagac gaaatgcagc aatcgctggg gcgcaacccg gatggtaagc
    45361 atctgatcat tctgcatacc aaaggctcac actttaacta cacccagcgt tacccgcgca
    45421 gctttgcgca gtggaagccg gaatgtattg gtgttgatag cggctgtacc aaagcgcaga
    45481 tgatcaactc atacgacaac tcggtgacct atgtggatca ctttatttcc agcgtgattg
    45541 atcaggttcg cgataagaaa gcgattgtgt tctatgcagc tgaccatggt gagtcaatta
    45601 atgaacgcga gcacctgcac ggcacgccgc gtgaactggc accgccggaa cagttccgcg
    45661 tgccgatgat ggtctggatg tcggataaat atctggaaaa tccggtcaat gcgcaggcat
    45721 ttgcgcagct gaaaaaagaa gctgacatga aagtgccacg ccgtcacgta gagctgtacg
    45781 acaccatcat gggttgtctt ggctatactt caccggatgg tggaattaac gaaaacaaca
    45841 actggtgcca catcccgcag gcaagagagg cggcggctaa ctaagaggcc tgctgacttt
    45901 ctcgccgatc aaaaggcatt ttgctattaa gggattgacg agggcgtatc tgcgcagtaa
    45961 gatgcgcccc gcattcggtg attggcgcag cctggtagcg cacttcgttc gggacgaagg
    46021 ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa aagcctgctc aacgagcagg
    46081 cttttttgca tttgtagcct atgaggatga gaacctccgg gggcagggag gttcgactcg
    46141 agcgaagcga gagaacgttg cgccagcaac ggcccgcagg gcgagccacg aagtggcgag
    46201 taatcctcta tcaccgacca aattcgaaaa gcctgctcaa cgagcaggct tttttgcatc
    46261 tgtagctcat gaggaagaga acccccgggg gcggggaggt tcgactcgac gtaggtctga
    46321 taagacgcgc cagcgtcgca tcaggcaacg gcaccgcact tattgtcgga tgcggcgtga
    46381 acgccttatt cgatctacac accacacatt tccctgtcaa atttgcgccc catatctcac
    46441 tatccattaa cggatttgtg acagaaacca ttgtcttttt ttatatttgc ttaaatcttt
    46501 ttttgcgtta cttatggctg gcgctggcat tttccgctgt gcgttttagc gttcatggta
    46561 ttaattgctc agatatccac cttttcctca gatttttttc attaaatgca taaaacttaa
    46621 tcaccaaatg ttgctaaata ttaagctgat tgttctggtt gtgttggcgt agcacaaatt
    46681 tctctgctgc aaatagctgt ttgaagtttt tttaatcttt gtttgtggat tctcaccgtt
    46741 ggtgtaaatc ccggttggct gtattgacgt tttcacattc tgttgacaga ttgtaggtca
    46801 cgaggggcat tttatggagg atccgcactg ttacactgat gttaattagt acggcatccc
    46861 cacctcataa cgttgacccg accgggcaaa aaacaaaaaa ggtcaggcag cgacaaccca
    46921 ctgcaaaggg ttaaaacaac aaacatcaca attggagcag aataatgcgt atttccttga
    46981 aaaagtcagg gatgctgaag cttggtctca gcctggtggc tatgaccgtc gcagcaagtg
    47041 ttcaggctaa aactctggtt tattgctcag aaggatctcc ggaagggttt aacccgcagc
    47101 tgtttacctc cggcaccacc tatgacgcct cttccgtacc gctttataac cgtctggttg
    47161 aatttaaaat cggcaccact gaagtgatcc cgggcctcgc tgaaaagtgg gaagtcagcg
    47221 aagacggtaa aacctatacc ttccatctgc gtaaaggtgt gaagtggcac gacaataaag
    47281 aattcaaacc gacgcgcgaa ctgaatgccg atgacgtagt gttctcgttc gatcgtcaga
    47341 aaaacgcgca aaacccgtac cataaagtct ctggcggcag ctacgaatac ttcgaaggca
    47401 tgggcttacc agagctgatc agcgaagtga aaaaggtgga cgataacacc gttcagtttg
    47461 tgctgactcg cccggaagcg ccgttcctcg ctgacctggc aatggacttc gcctctattc
    47521 tgtcaaaaga atatgctgac gcgatgatga aagccggtac accagaaaaa ctggacctca
    47581 acccaatcgg taccggcccg ttccagttac agcagtacca gaaagattcc cgtattcgct
    47641 ataaagcgtt tgatggctac tggggcacca aaccgaagat cgatacgctg gtcttctcta
    47701 ttactcctga cgcttccgtg cgttatgcga aattgcagaa gaacgaatgc caggtgatgc
    47761 cgtacccgaa cccggcagat atcgcccgca tgaagcagga taaatccatc aacctgatgg
    47821 aaatgccggg gctgaacgtc ggttatctct cgtataacgt gcagaaaaaa ccactcgatg
    47881 acgtgaaagt tcgccaggct ctgacctacg cggtgaacaa agacgcgatc atcaaagcgg
    47941 tttatcaggg cgcgggcgta tcagcgaaaa acctgatccc gccaaccatg tggggctata
    48001 acgacgacgt tcaggactac acctacgatc ctgaaaaagc gaaagccttg ctgaaagaag
    48061 cgggtctgga aaaaggtttc tccatcgacc tgtgggcgat gccggtacaa cgtccgtata
    48121 acccgaacgc tcgccgcatg gcggagatga ttcaggcaga ctgggcgaaa gtcggcgtgc
    48181 aggccaaaat tgtcacctac gaatggggtg agtacctcaa gcgtgcgaaa gatggcgagc
    48241 accagacggt aatgatgggc tggactggcg ataatgggga tccggataac ttcttcgcca
    48301 ccctgttcag ctgcgccgcc tctgaacaag gctccaacta ctcaaaatgg tgctacaaac
    48361 cgtttgaaga tctgattcaa ccggcgcgtg ctaccgacga ccataacaaa cgcgttgaac
    48421 tgtacaagca agcgcaggtg gtgatgcacg atcaggctcc ggcactgatc atcgctcact
    48481 ccaccgtgtt tgaaccggta cgcaaagaag ttaaaggcta tgtggttgat ccattaggca
    48541 aacatcactt cgaaaacgtc tctatcgaat aattaaaagc catacaagac tgatggcaaa
    48601 ggcaaaactg cctgatgcgc tccgcttatc aggcctacga aaactctgca atgtattgag
    48661 tttgcacgat tttgtaggtc ggataaggcg tttgcgccgc atccggcata aacaaagcgc
    48721 tctttgtcaa caatctgcat acccggtggc gctgtgcctc tgcgtgcagc gccatccggc
    48781 agcaatactt cattccctgt tccacaaggg cgggaattga tttgtgagca atacagacac
    48841 gcagttccag gctgcgggtc attatagaga atccgggtta tgttgcagtt tattctccga
    48901 cgtttgggac tcgtcatccc cacgtttatc ggtattaccc ttctcacatt tgcctttgtc
    48961 cacatgatcc cgggcgatcc ggtgatgatc atggcgggcg agcgtgggat ctccccagag
    49021 cgtcacgcgc agctgctggc tgaactcggc ttagataaac cgatgtggca gcagtatctc
    49081 cattacattt ggggcgtaat gcacggcgat ttaggcattt cgatgaaaag ccgcatcccg
    49141 gtatgggaag agttcgtgcc gcgcttccag gccacgctgg aacttggcgt ctgcgcgatg
    49201 atttttgcta cggcagtcgg tattccggtt ggtgtgctgg ctgcggttaa acgcggttcc
    49261 attttcgatc acacggcggt tggcctggcg ctgacaggtt attcaatgcc tatcttctgg
    49321 tggggcatga tgctgatcat gctggtttcg gtgcactgga acctgacgcc cgtctccggt
    49381 cgcgtgagcg acatggtgtt cctcgatgat tccaatccgt taaccggttt tatgctgatc
    49441 gacaccgcca tctggggtga agacggtaac tttatcgatg ccgtcgccca tatgatcttg
    49501 cccgccattg tgctgggtac tatcccgctg gcggtcattg tgcgtatgac acgctcctcg
    49561 atgctggaag tgctgggtga agattacatc cgcaccgcgc gcgccaaagg gttgacccgc
    49621 cttagggtga ttatcgtcca tgcgctgcgt aacgcgatgc tgccggtggt gaccgttatc
    49681 ggcctgcagg tgggaacgtt gctggcgggg gcgattctga ccgaaaccat cttctcgtgg
    49741 cccggtctgg ggcgctggtt gattgacgca ctgcaacgcc gcgactatcc ggtagtgcag
    49801 ggcggcgtat tgctggtggc gacgatgatt atcctcgtca acttgctggt cgatctgctg
    49861 tacggcgtgg tgaacccgcg tattcgtcat aagaagtaag gggacatcat gtcacaggtt
    49921 actgaaaata aagtgattag cgcaccggtg ccgatgaccc cgttacagga gttctggcac
    49981 tattttaaac gcaacaaagg cgcggtcgtc gggctggttt acgtcgtcat cgtgctgttt
    50041 atcgcgatct ttgccaactg gattgcaccc tataacccgg cggaacagtt ccgcgatgca
    50101 ctgctcgccc cgccagcctg gcaggaaggc ggcagcatgg cgcacttgct gggcaccgat
    50161 gacgtaggcc gtgatgtgct gtcgcgtctg atgtacggtg cgcgattgtc actgctggtt
    50221 ggctgtctgg tagttgtgtt atcgctgatt atgggcgtta ttctcggcct gatcgccggt
    50281 tacttcggcg gcctggtcga taacatcatc atgcgcgtgg tcgatatcat gctggcgctg
    50341 ccaagtctgc tgttggcact ggtgctggtg gcgattttcg gcccgtcgat tggtaacgcc
    50401 gcgctggcgc tgacctttgt tgccctgccg cactatgtgc gcttaacccg cgccgccgtg
    50461 ctggtggaag ttaaccgcga ttacgttacc gcgtctcgcg tggcaggtgc cggggcgatg
    50521 cgtcagatgt ttattaacat cttcccgaac tgccttgcgc ctctgattgt tcaggcgtcg
    50581 ctcggtttct ctaacgccat tctcgatatg gctgctcttg gtttcctcgg catgggggcg
    50641 cagccgccaa cgccggagtg gggcaccatg ctctccgacg tgttgcagtt cgcgcaaagc
    50701 gcctggtggg tcgtgacctt cccgggtctg gcgatcctgc tgacggtgct ggcatttaac
    50761 ctgatgggtg acggtctgcg tgacgcgctc gatcccaaac tgaagcagta agaggttcga
    50821 gatggcgtta ttaaatgtag ataaattatc ggtgcatttc ggcgacgaaa gcgcaccgtt
    50881 ccgcgccgta gaccgcatca gctacagcgt aaaacagggc gaagtggtcg ggattgtggg
    50941 tgagtccggc tctggtaaat cggtcagttc gctggcgatt atggggctga ttgattatcc
    51001 gggccgcgta atggcggaaa agctggagtt taacggtcag gatttgcagc gtatctcgga
    51061 aaaagagcgc cgcaaccttg tgggtgccga agtggcgatg atcttccagg acccgatgac
    51121 cagccttaac ccgtgctaca ccgtgggttt ccagattatg gaagcgatta aggtgcatca
    51181 gggcggtaat aagagcactc gtcgtcagcg ggcgattgac ttgttgaatc aggtcggtat
    51241 tcccgatccg gcttcgcgtc tggatgttta cccgcatcag ctttccggcg gcatgagcca
    51301 gcgcgtgatg attgccatgg cgattgcctg tcggccaaaa ctgctgattg ccgatgaacc
    51361 aaccactgcg ctggacgtga ccattcaggc gcaaatcatt gaactactgc tggagctaca
    51421 gcagaaagag aacatggcgc tggtgttaat tacccatgac ctggcgctgg tggcggaagc
    51481 ggcacataaa atcatcgtga tgtatgccgg tcaggtggtg gaaacgggcg atgcgcacgc
    51541 catcttccat gcgccgcgtc acccgtatac tcaggcattg ctgcgtgcgc tgccggaatt
    51601 tgctcaggat aaagaacgtc tggcgtcgtt gccaggtgtt gttcccggca agtatgaccg
    51661 cccgaacggc tgcctgctta acccgcgctg cccctatgcc actgacagat gtcgcgctga
    51721 agaaccggcg ctgaatatgc ttgctgacgg gcgtcagtcc aaatgccatt acccacttga
    51781 tgatgccggg aggccgacac tatgagtacg caagaggcca cctcgcaaca accgctgttg
    51841 caggctatcg acctgaaaaa acattatccg gtgaagaaag gtatgttcgc gccggaacgt
    51901 ttggttaaag cgctggacgg cgtttcgttt aaccttgaac gcggcaaaac gctggcggta
    51961 gtaggggaat ctggctgcgg taaatcgacc ctcggtcggt tgctgacgat gatcgaaacg
    52021 cccaccggtg gtgaactgta ttaccaaggg caggatctgc tcaagcacga tccgcaggcg
    52081 cagaagctgc gtcggcagaa aatccagatt gtcttccaga acccttacgg ttcgctgaat
    52141 ccacgtaaaa aagtcgggca aattcttgag gagccgctgc tgattaatac cagcttaagc
    52201 aaagaacagc gtcgggaaaa agccctgtcg atgatggcga aagtcggcct gaaaaccgaa
    52261 cattatgacc gctatccgca tatgttctcc ggcggtcagc gtcagcgtat cgccattgcc
    52321 cgaggtctga tgctcgaccc ggatgtggtg attgccgatg agccggtttc cgcgctggac
    52381 gtctcagtgc gcgcgcaggt gctgaatctg atgatggatt tgcagcagga gttggggctg
    52441 tcttatgtct ttatctcgca cgacctgtcg gtggtagagc acattgctga tgaagtgatg
    52501 gtgatgtacc tgggccgctg cgtggagaag ggaacgaagg accaaatctt caataacccg
    52561 cgtcatccgt acactcaggc gctactctcc gcgacgccgc gcctgaaccc ggacgatcgc
    52621 cgcgagcgca tcaagctcac cggtgaactg ccaagcccac tgaatccacc gccgggctgc
    52681 gcgtttaacg cccgctgtcg tcggcgcttc ggcccctgca cccagttgca gccgcagcta
    52741 aaagactacg gcggtcaact ggtagcttgt tttgctgttg atcaggatga aaatccgcag
    52801 cgttaactca caaaaccggg gccgtgcgct ccggtttact caatcagctt caacagtggc
    52861 gacaagcata gcaaaatgcc gtaaagcaga atcagccagg ctttaaaccc gcgaagtttt
    52921 tccagttgtg cgactttata gatcaggaaa aatggaatga ggcacgacac aatgccatat
    52981 aacgggcttc ccaactgaaa gaacaccagc accgatacac gaaacgagac ccaaatcgtc
    53041 aacgtaatga cgataaaagc gcagatcgcc agagtcagca tgcgtgagtt aattttcttg
    53101 gtatcaataa ttcggcttaa cagattgaga ataatgcctt taatggcctc gtggaaaccg
    53161 agataaatgc caaagaatgc ggtcagtacg gcaaagatat tcagcaccgt cgaggtgata
    53221 tgaatgatat gcccagggat cacctgcgcg gccagtgcca gtgccgagat attttgttca
    53281 aaggcagaaa cggcttcttc gtggctaatt gagaaggtaa acgaaaaggc aaaaaatagg
    53341 atcaccgcga tgagcgtgac ataactaatt cggtgggtac gcagcgcgag ccgggttgcc
    53401 agtactttat ccgcttcccg tttgcggtag gcaatattca tcgggtttag cacctgaata
    53461 aataccgcag aaaagaagca aaaaggaata gtaagcagga cgtcgcggaa gaagacggag
    53521 gcttgcggga aggcggttat attggcgaaa ttccagtgcg ggatcatcgc aaaaccgaag
    53581 acgacaataa tccctacttt gaccaccacc attggcccgg aaatcttaaa cagtaatcgt
    53641 tcaccaccag acgcaattgc caccagtacg gcgaaaatgg cgactttata aagtagagat
    53701 tgtgaaagat cggcatcggt taaaccgaag gttttcaggt acgaggcgct gtcgaaaacc
    53761 acggagagag agtagataaa aatcccgtgg ataatcatca aaaagtagat aaccccgagg
    53821 aaaattcccc agttcttccc caggtaatga ctgataatat cggtgtagtc attacaggaa
    53881 tcgctttcag aaagggtttt taaataaatg tcctgcacta cccaggtggc aggataagca
    53941 atgatcgctg cggtaataaa tacccaaatt cccttcaagc caatttgtac tggcatcagc
    54001 acggttccgg caccaatcgc catgcctatg cataataaaa cccagccgaa gtcgtagcgt
    54061 gtaaacggca ttttctgatc gtgtttcggt agtgtgttgt gctgcataaa gtcctgtcca
    54121 gaaagaagtc ggcctcgcaa aaatgaataa acccagtttc tcttgcgcaa caggtggaga
    54181 agtcgaatac gcagtatgta aaaaatgagg caacagattc actggggagg tcgcgaggag
    54241 gcgcaattct acaaaattgc gcatttcact accagtctgg agtcagcccg gatatgaact
    54301 gcaactaatg caaataaaga aattaagctg gaaggcatca cgcaatgagg aagattgccg
    54361 ggtgctgacg gtaaaaagca atcgtaaaat cagcggcaag ccggaaaggt tccggtgagg
    54421 cgcaatgttg cgggggctta atccctggtg gcattggttg ctggagagag aaaacccccg
    54481 cacgttgcag gtatgcacct gacaacatca cgggggctaa tcttgaacct agaccactca
    54541 agaatagccg cgaaacgttg tcattacaac acaggcggct atatgacgct cgcagaactg
    54601 ggcatggcct tctggcatga tttagcggct ccggtcatag ctggcattct tgccagtctg
    54661 atcgtgaact ggctgaacaa gcggaagtaa cgtgtcagac gggcgtcagg ctgccgtaat
    54721 ggcaatttgc gcccggatca ggccgcaggg ataaaactct gcggcctttt tcgtaagaaa
    54781 aatgcaacgg ttgcaagcgg ataatattct gcggaatgca cagcacagag tgaagaagtg
    54841 acggatttcg gagctgaaaa acagtcgtag aatcagcggc aagccggaaa ggttccggtg
    54901 aggcgcaatg ttgcgggggc ttaatccctg gtggcattgg ttgctggaga gagaaaaccc
    54961 ccgcatattg caggtatgca cctgacaaca tcacgggggc cattcttgaa cctacacaac
    55021 tcaagaatag ccgcgaaacg ttgtcattac aactcaggcg gctatatgac gctcgcagag
    55081 ctgggcatgg ccttctggca tgatttagcg gctccggtca ttgctggcat tcttgccagc
    55141 atgatcgtga actggctgaa taagcggaag taacgtgtca gacgggcgtc aggctgccgt
    55201 aatggcaatt tgcgcccgga ccaggccgca gggataaaac tctgcggcct ttttcgtaag
    55261 aaaaatgcaa cggttgcaag cggataatat tctgcggaat gcgcagcaca gagtgaagaa
    55321 gtgatggatt ccggaacaga aaaacagtcg tagaatcagc ggcaagccgg aaaggttccg
    55381 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
    55441 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatctagac
    55501 cattcaagaa tagccgcgaa acgttgtcat tacaactcag gcggctatat gacgctcgca
    55561 gagctgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
    55621 agcatgatcg tgaactggct gaataagcgg aagtaacgtg tcagacgggc gtcaggctgc
    55681 cgtaatggca agttgcgccc ggatcaggcc gcagggataa aactctgcgg cctttttcgt
    55741 aagaaaaatg caacggttgc aagcggataa tattctgcgg aatgcgcagc acagagtgaa
    55801 gacgtgacgg attccggagc agaaaaacag tcgtagaatc agcggcaagc cggaaaggtt
    55861 ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg cattgtttgc tggagagaga
    55921 aaacccccgc acgttgcagg tatgcacctg acaacaccac gggggccatt cttgaaccca
    55981 aacaactcaa gaatagccgc gaaacgttgt cattacaact caggcggcta tatgacgctc
    56041 gcagagctgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
    56101 gccagcatga tcgtgaactg gctgaacaag cggaagtaac gtgtcatgcg ggcgtcaggc
    56161 tgccgtaatg gcaatttgcg cccggatcag gccgcagggg tgaaactctg cggccttttt
    56221 cgttcttatt gcgggtaagg cacccagtcg ccgccgttca ggcgaacgta cggtttatcc
    56281 tggtattgaa taactactgc atttgagttc tcggagaccg gtgctgtttg tggcaaccca
    56341 ctggtgagtt ttttccagtc aacattgtct tcggagaaaa tcttgccatc aagaacgcga
    56401 accaccagat cggagatagc caggaagctg ctcggttggt cgatgacaat cggtgccccc
    56461 tgatgtggtg ccttcatgcc gaagaatttc accccaacgg ggacgtctgt gatagacggg
    56521 ctagggatat cacgtaggcc agatacctgc attctgtcgc ccttcagcgc gccgccgtgt
    56581 tccggcacca cgaccaccat cactttacga cccgatttct ccagttcagt aaagaaggcg
    56641 tccagttcat caaagaattt ctgcgcccgc gctttgtaat ccgctgtttt gctgacgccc
    56701 ggataatggt tgccgtcatg cagtggaagc gtgttgtaga acgtggcact acggctgttt
    56761 ttatcttttt cggtaatgtc cagccagcgg ttaagcacgg cggtatcgtc ataaaccggc
    56821 gaaccatcaa agcccagcaa aataaccggc agatttgttt gatccatcaa ttcggtctgc
    56881 atgccgccat tttcgcgaac ttctttcaaa aaaccgccga actggccgtt atgtcccatc
    56941 atcaggtgct gggtaaagcc cagtttcgac aggttatcaa acagatagca gtcgttattt
    57001 gccggttgat acagattagt gtgcgaagtc tgcccgcagc tggcgcgcag taaacggatc
    57061 gccgccgggc cactgtagga ggtggcggag ttaaagttct tgaactcaat atcgaaatgc
    57121 gaccacagcg gatgcgacat caacccggcg gcttcaatat ccgaccagga aagcgaacag
    57181 atattaatca ccagtagttc aaatggctga gcatcagcgg gcagcgaaga cgggaaggtc
    57241 gatttacgtt tcgcctccgc gttatagaaa ttatttagcc aggcgttaag gttcgccgtt
    57301 gttggcggtg cagtttgtgc gggcatatca cccactaccg gtgcgccacc cgtcgccgca
    57361 acggttgccg ctgcgttacc acccgtcgtt gttacagtgg tcgtcggttg accggctggc
    57421 cacaaggaga aacttggtcc cgccagggta agtacgttca gccatagcag aatggcaacc
    57481 acaaaaacgg taatgcgaat ccattgtgac aggaataacc aggccacgaa taaaacaaaa
    57541 atggccccaa tcatctgcca gttaataaag cgtgtgacaa ggtcgattaa ataatcggta
    57601 ctgaaccccg ccacctgcga accctggctc attatgcttt ccgggccagg caaccaggtg
    57661 tcatgccaga acaaagcaaa gccgatcggc agggcaatcc agtggcgcaa gcgatgcagg
    57721 ctgtagcgcg gaatgggcat cagcagaaac gcggcaaaga ccaaattgag gagcggatgg
    57781 aagttaagat atcccgccca caacaggccg aacttaacca gaaaatagaa gttccagccg
    57841 gaaaggccgc gccagtattg ccagagggaa gaaggcatgg cggtattttg cgtaaattga
    57901 gtcatttttt ggttgccctg gctttttccg tgcggcgtaa cgtcccggcc ggtttaacat
    57961 aacgaggttt agcaaagaac aaacgtaagg tgtcgcgaat gcgtcgcaaa gagtgccgcg
    58021 ccagatagcc cagcgggaaa aatatcagtg cgcaaacgac aataatttca atgatatcgc
    58081 tgatggtcat catgatgagc gctccacagc atcatctaac agtcgcattg gttctggtat
    58141 tcgtcgccag tggcgaccat cgtgctcggc attgatgacc ggtttagaac tttgcgttaa
    58201 aggcagcggc atgccccatt gttctggggc aagcaggcgc atctgcacca gctcggcact
    58261 gatttgatca tcttcaaacc agaccatacg gtttgagaaa atgtcgccag taggcaatgg
    58321 gaaaatatga ttcaacgcgg tatccagatc gttaatccga cagaatgaga gaaacagcac
    58381 cagccgatta ccgccaatgg tcatgatatc gccggtgcga ttagggcgac atagcgtcag
    58441 ggcttgctca acgcggatac ccggtaccgg acgtagggca accagaacgc ctttaccgtg
    58501 ggcaggtaat agagggttat tcatcatgtt gttgacggca ttacagaaca catcccactt
    58561 ctggaaacca cgcagtttga gcggctgggt cattgacagc aaggtagtga tatcttccgg
    58621 cacatagcga ctaaacttct gcccttgcac gctttcgatc atcgtcagac agcgggagag
    58681 tggcgcattc cacgggataa ccatatttgc accgcaggcc aataataaac gttcatcggt
    58741 ggcgcgcagg ctagcggtat tttcccgtac gaggattttc atcgcactac cgcgctggcg
    58801 acgcagagta tgaatgctgc gggccagtgg ctcgatttgc gcattttgct gtaaagaaaa
    58861 gaccaccgtc gccgcctgag cggtacgggc ttcattgaac aggacttcgt tattgttgaa
    58921 cagttgccag tgttccgata gcggcggcgc accttccagt acagcaacat tactcagaat
    58981 gcgtttttcg tcgctgcgtg gttggatttc cgcctcctgg tgttgaacta atgtccagca
    59041 accattttgt tgctgaacgc taagctgctg acgggcgctg accccttttt cgttgcacca
    59101 gaaagcaata tccagcaaat gttggtcgcc ctgaaaacgc aaactggcaa gaccaaaaag
    59161 tgaacggtat tcctcaagta acaatgaaaa ttgtttatcg ttattatttc cggaattaat
    59221 taccaaaagc gaacaatggt ttagccggct ccatttattc attttatcca accatgagcg
    59281 aagccgctcg gcaggaatgt tttgccatgc gttatttgcg caaacaagaa taaaaaggta
    59341 attatgggga tcaatagaac actgcaaatc acgggccaaa tagtatagac ctttttcatg
    59401 atttagcatt gaaaataatg ttatttttcc cggcccttga gaatcatcta attgaaagat
    59461 tttcgctgga tcgctgtcca tgctaatgac cgcgacgtgt gcggtttcag cctgggatgc
    59521 aattgtttga ttcgccagac tgatagcatc ttcatggcga tcgacgttaa accaccagac
    59581 gccgcctgct ggcatatgtc gcagctcatc ccataatgat gagataccga tagagaatac
    59641 agggtccaca atgtccctca tgtcaacatt ttgtctgatt atcagtttac tagcgaaagc
    59701 taagaaataa acctaccatt gaaattaaaa cttatcagat ttagcatgtg aatcaattta
    59761 gcaggtcaca agacatgtga ttttagtctt ttcgttttta actttttctg ttgcgtaacg
    59821 gaaagtcaaa aagtgagcac attcccgtct cgccgtaaac aataaaccga cgaaatgctc
    59881 acagttagaa ttgtctcatg aacggtacgg ttatttcata gggatcaaga aaaatgaata
    59941 acaatgaacc agatactctg cctgatcccg cgataggcta tatcttccag aatgatattt
    60001 tggcgttaaa gcaggcattt tcactgcctg atattgatta tgccgatatt tcccaacgcg
    60061 aacagttggc cgcggcatta aaacgctggc cgttgctggc agagtttgcg caacaaaagt
    60121 aggggattgg tgaatggc
//
LOCUS       DAZGHF010000014        59140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGHF010000014 DAZGHF010000000
VERSION     DAZGHF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.014"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(339..545)
                     /gene="ybfA"
                     /locus_tag="ACHK3A_000958"
     CDS             complement(339..545)
                     /gene="ybfA"
                     /locus_tag="ACHK3A_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO8739083.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            858..947
                     /gene="kdpF"
                     /locus_tag="ACHK3A_000959"
     CDS             858..947
                     /gene="kdpF"
                     /locus_tag="ACHK3A_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO8739084.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            947..2620
                     /gene="kdpA"
                     /locus_tag="ACHK3A_000960"
     CDS             947..2620
                     /gene="kdpA"
                     /locus_tag="ACHK3A_000960"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO8739085.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFIICTGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMYDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLALMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2643..4691
                     /gene="kdpB"
                     /locus_tag="ACHK3A_000961"
     CDS             2643..4691
                     /gene="kdpB"
                     /locus_tag="ACHK3A_000961"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO8739086.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTFISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPVDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4700..5272
                     /gene="kdpC"
                     /locus_tag="ACHK3A_000962"
     CDS             4700..5272
                     /gene="kdpC"
                     /locus_tag="ACHK3A_000962"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO8739087.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5265..7949
                     /gene="kdpD"
                     /locus_tag="ACHK3A_000963"
                     /pseudo
     CDS             5265..7949
                     /gene="kdpD"
                     /locus_tag="ACHK3A_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7946..8623
                     /gene="kdpE"
                     /locus_tag="ACHK3A_000964"
     CDS             7946..8623
                     /gene="kdpE"
                     /locus_tag="ACHK3A_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO8739088.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDSAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8857..8961
                     /locus_tag="ACHK3A_000965"
     CDS             8857..8961
                     /locus_tag="ACHK3A_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739089.1"
                     /translation="MENNSRTMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9219..11417
                     /gene="speF"
                     /locus_tag="ACHK3A_000966"
     CDS             9219..11417
                     /gene="speF"
                     /locus_tag="ACHK3A_000966"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO8739090.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVT
                     RGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEFAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11414..12733
                     /gene="potE"
                     /locus_tag="ACHK3A_000967"
     CDS             11414..12733
                     /gene="potE"
                     /locus_tag="ACHK3A_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO8739091.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKIFSRVSKVDAPVQGMLTIVIIQS
                     GLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12790..14430)
                     /gene="pgm"
                     /locus_tag="ACHK3A_000968"
     CDS             complement(12790..14430)
                     /gene="pgm"
                     /locus_tag="ACHK3A_000968"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO8739092.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14456..15001)
                     /gene="seqA"
                     /locus_tag="ACHK3A_000969"
     CDS             complement(14456..15001)
                     /gene="seqA"
                     /locus_tag="ACHK3A_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO8739093.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15186..15950
                     /gene="ybfF"
                     /locus_tag="ACHK3A_000970"
     CDS             15186..15950
                     /gene="ybfF"
                     /locus_tag="ACHK3A_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8739094.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16090..16383
                     /gene="ybfE"
                     /locus_tag="ACHK3A_000971"
     CDS             16090..16383
                     /gene="ybfE"
                     /locus_tag="ACHK3A_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO8739095.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16523..17053
                     /gene="fldA"
                     /locus_tag="ACHK3A_000972"
     CDS             16523..17053
                     /gene="fldA"
                     /locus_tag="ACHK3A_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO8739096.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17263..17349
                     /gene="uof"
                     /locus_tag="ACHK3A_000973"
     CDS             17263..17349
                     /gene="uof"
                     /locus_tag="ACHK3A_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO8739097.1"
                     /translation="MIRIISRANSVTSPNEVNRLVTGQIPHD"
     gene            17342..17788
                     /gene="fur"
                     /locus_tag="ACHK3A_000974"
     CDS             17342..17788
                     /gene="fur"
                     /locus_tag="ACHK3A_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO8739098.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17872..18198)
                     /gene="chiQ"
                     /locus_tag="ACHK3A_000975"
     CDS             complement(17872..18198)
                     /gene="chiQ"
                     /locus_tag="ACHK3A_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO8739099.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18248..19654)
                     /gene="chiP"
                     /locus_tag="ACHK3A_000976"
     CDS             complement(18248..19654)
                     /gene="chiP"
                     /locus_tag="ACHK3A_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO8739100.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDIYDGTAWLQALTFGYRAADVVDLRLEGSWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20103..21767)
                     /gene="glnS"
                     /locus_tag="ACHK3A_000977"
     CDS             complement(20103..21767)
                     /gene="glnS"
                     /locus_tag="ACHK3A_000977"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO8739101.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGSFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSCEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(21970..23916)
                     /gene="nagE"
                     /locus_tag="ACHK3A_000978"
     CDS             complement(21970..23916)
                     /gene="nagE"
                     /locus_tag="ACHK3A_000978"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO8739102.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDVMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            24249..25049
                     /gene="nagB"
                     /locus_tag="ACHK3A_000979"
     CDS             24249..25049
                     /gene="nagB"
                     /locus_tag="ACHK3A_000979"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO8739103.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25109..26257
                     /gene="nagA"
                     /locus_tag="ACHK3A_000980"
     CDS             25109..26257
                     /gene="nagA"
                     /locus_tag="ACHK3A_000980"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO8739104.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            26266..27486
                     /gene="nagC"
                     /locus_tag="ACHK3A_000981"
                     /gene_synonym="nagR"
     CDS             26266..27486
                     /gene="nagC"
                     /locus_tag="ACHK3A_000981"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO8739105.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27534..28286
                     /gene="nagD"
                     /locus_tag="ACHK3A_000982"
     CDS             27534..28286
                     /gene="nagD"
                     /locus_tag="ACHK3A_000982"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO8739106.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28542..30206
                     /gene="asnB"
                     /locus_tag="ACHK3A_000983"
     CDS             28542..30206
                     /gene="asnB"
                     /locus_tag="ACHK3A_000983"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO8739107.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            30587..30663
                     /locus_tag="ACHK3A_000984"
     tRNA            30587..30663
                     /locus_tag="ACHK3A_000984"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30621..30623,aa:Met,seq:cat)
     gene            30672..30756
                     /locus_tag="ACHK3A_000985"
     tRNA            30672..30756
                     /locus_tag="ACHK3A_000985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30706..30708,aa:Leu,seq:tag)
     gene            30780..30854
                     /locus_tag="ACHK3A_000986"
     tRNA            30780..30854
                     /locus_tag="ACHK3A_000986"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30812..30814,aa:Gln,seq:ttg)
     gene            30889..30963
                     /locus_tag="ACHK3A_000987"
     tRNA            30889..30963
                     /locus_tag="ACHK3A_000987"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30921..30923,aa:Gln,seq:ttg)
     gene            30979..31055
                     /locus_tag="ACHK3A_000988"
     tRNA            30979..31055
                     /locus_tag="ACHK3A_000988"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31013..31015,aa:Met,seq:cat)
     gene            31104..31178
                     /locus_tag="ACHK3A_000989"
     tRNA            31104..31178
                     /locus_tag="ACHK3A_000989"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31136..31138,aa:Gln,seq:ctg)
     gene            31216..31290
                     /locus_tag="ACHK3A_000990"
     tRNA            31216..31290
                     /locus_tag="ACHK3A_000990"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31248..31250,aa:Gln,seq:ctg)
     gene            complement(31444..32619)
                     /gene="ubiF"
                     /locus_tag="ACHK3A_000991"
     CDS             complement(31444..32619)
                     /gene="ubiF"
                     /locus_tag="ACHK3A_000991"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO8739108.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            32765..34189
                     /gene="miaB"
                     /locus_tag="ACHK3A_000992"
     CDS             32765..34189
                     /gene="miaB"
                     /locus_tag="ACHK3A_000992"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO8739109.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34342..35382
                     /gene="ybeZ"
                     /locus_tag="ACHK3A_000993"
     CDS             34342..35382
                     /gene="ybeZ"
                     /locus_tag="ACHK3A_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO8739110.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35379..35846
                     /gene="ybeY"
                     /locus_tag="ACHK3A_000994"
     CDS             35379..35846
                     /gene="ybeY"
                     /locus_tag="ACHK3A_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO8739111.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            35936..36814
                     /gene="corC"
                     /locus_tag="ACHK3A_000995"
     CDS             35936..36814
                     /gene="corC"
                     /locus_tag="ACHK3A_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO8739112.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            36839..38377
                     /gene="lnt"
                     /locus_tag="ACHK3A_000996"
     CDS             36839..38377
                     /gene="lnt"
                     /locus_tag="ACHK3A_000996"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO8739113.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKH"
     gene            complement(38455..40332)
                     /locus_tag="ACHK3A_000997"
     CDS             complement(38455..40332)
                     /locus_tag="ACHK3A_000997"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO8739114.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLLSSVFLHSSVSHLLMNMFALLVVGGAVERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLSSGAGTHHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWFVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANAVSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVTDIPLEKYDGTGTSGKPGAWV
                     MALSPDEKTLLIPGMVRGDIVRINTITHQKEDFPTGDARGIVSAMGFRPKNGEIIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            40787..41695
                     /gene="gltI"
                     /locus_tag="ACHK3A_000998"
     CDS             40787..41695
                     /gene="gltI"
                     /locus_tag="ACHK3A_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO8739115.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            41865..42605
                     /gene="gltJ"
                     /locus_tag="ACHK3A_000999"
     CDS             41865..42605
                     /gene="gltJ"
                     /locus_tag="ACHK3A_000999"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO8739116.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            42605..43279
                     /gene="gltK"
                     /locus_tag="ACHK3A_001000"
     CDS             42605..43279
                     /gene="gltK"
                     /locus_tag="ACHK3A_001000"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO8739117.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            43279..44004
                     /gene="gltL"
                     /locus_tag="ACHK3A_001001"
     CDS             43279..44004
                     /gene="gltL"
                     /locus_tag="ACHK3A_001001"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO8739118.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            44122..45057
                     /gene="rihA"
                     /locus_tag="ACHK3A_001002"
     CDS             44122..45057
                     /gene="rihA"
                     /locus_tag="ACHK3A_001002"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO8739119.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLCESEEPITIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(45102..45584)
                     /gene="ybeL"
                     /locus_tag="ACHK3A_001003"
     CDS             complement(45102..45584)
                     /gene="ybeL"
                     /locus_tag="ACHK3A_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO8739120.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            45819..48401
                     /gene="leuS"
                     /locus_tag="ACHK3A_001004"
     CDS             45819..48401
                     /gene="leuS"
                     /locus_tag="ACHK3A_001004"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO8739121.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            48416..48997
                     /gene="lptE"
                     /locus_tag="ACHK3A_001005"
     CDS             48416..48997
                     /gene="lptE"
                     /locus_tag="ACHK3A_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO8739122.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            48997..50028
                     /gene="holA"
                     /locus_tag="ACHK3A_001006"
     CDS             48997..50028
                     /gene="holA"
                     /locus_tag="ACHK3A_001006"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO8739123.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAV
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHMPLRALFDKH
                     RVWQNRRGMMGEALNRLSHPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            50030..50671
                     /gene="nadD"
                     /locus_tag="ACHK3A_001007"
     CDS             50030..50671
                     /gene="nadD"
                     /locus_tag="ACHK3A_001007"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO8739124.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            50695..51306
                     /gene="cobC"
                     /locus_tag="ACHK3A_001008"
     CDS             50695..51306
                     /gene="cobC"
                     /locus_tag="ACHK3A_001008"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO8739125.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            51566..51883
                     /gene="rsfS"
                     /locus_tag="ACHK3A_001009"
     CDS             51566..51883
                     /gene="rsfS"
                     /locus_tag="ACHK3A_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO8739126.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            51887..52354
                     /gene="rlmH"
                     /locus_tag="ACHK3A_001010"
     CDS             51887..52354
                     /gene="rlmH"
                     /locus_tag="ACHK3A_001010"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO8739127.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            52385..54286
                     /gene="mrdA"
                     /locus_tag="ACHK3A_001011"
     CDS             52385..54286
                     /gene="mrdA"
                     /locus_tag="ACHK3A_001011"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO8739128.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            54289..55401
                     /gene="mrdB"
                     /locus_tag="ACHK3A_001012"
                     /gene_synonym="rodA"
     CDS             54289..55401
                     /gene="mrdB"
                     /locus_tag="ACHK3A_001012"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO8739129.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            55412..56500
                     /gene="rlpA"
                     /locus_tag="ACHK3A_001013"
     CDS             55412..56500
                     /gene="rlpA"
                     /locus_tag="ACHK3A_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO8739130.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGLYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            56640..57851
                     /gene="dacA"
                     /locus_tag="ACHK3A_001014"
     CDS             56640..57851
                     /gene="dacA"
                     /locus_tag="ACHK3A_001014"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO8739131.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            57961..58224
                     /gene="ybeD"
                     /locus_tag="ACHK3A_001015"
     CDS             57961..58224
                     /gene="ybeD"
                     /locus_tag="ACHK3A_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO8739132.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            58325..58966
                     /gene="lipB"
                     /locus_tag="ACHK3A_001016"
     CDS             58325..58966
                     /gene="lipB"
                     /locus_tag="ACHK3A_001016"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO8739133.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT        14187 a        15243 c        15334 g        14376 t
ORIGIN      
        1 tgggggcacc aatacgtacc ccggctgaac cctgaacaat gctaccgcca tactgcgtca
       61 tgtcgccctg acgcgccgcc ggttttccgc tcatctgctc atccttgttt tatcgggtta
      121 tttgtctgct gacaggcgaa accctacaga tttataagaa agccttaagt gagatatctg
      181 tcagataaga acaattagga aaatatttat atatatttca tgaatatatg tttcgattgg
      241 tataacggtg tctaaatatt cctctgaaca tcttcctgat acaaaaaaaa ccgccagcgt
      301 ttccgctggc ggttttcaat gtattcagaa ctcagcaatc agtaaaaatc accagtcgcc
      361 ttttcggcct gatccatcca caccggttta tcgctggttt tcgaccagac gcggtgcagg
      421 tagctgtaaa agcgggcgcg gtctttccag aaaagcatga aaggcagcgc cagaacgccc
      481 gctgcaaccg cataagtacg gcgtaaaaag ataagccatg caggatattc tctgtagagt
      541 tccatacttt tctccccctt ttgtatctac ccggtgaata gcaccggaaa aatgaatttg
      601 tttatctgat gaaaatagta ccgccttttg tgtaatttta ctactcatcc gaccacttat
      661 ttttgcttat tgatggttta tttacattca taccgtaatt aagttacaca aaggttaaat
      721 taatactaaa cattagttaa atcatggctt ttgccatttt tatacttttt ttacaccccg
      781 cccgcagatt tttgcgaaat ctttgcagcc agaattctac tcttccggta tcacttttag
      841 gccactggag gtgcactgtg agtgcaggcg tgatagccgg cgtgttgctg gtgtttttat
      901 tactgggtta tctggtttat gccctgatca atgcggaggc gttctgatgg ctgcgcaagg
      961 gttcttactg attgccacgt ttttactggt gttaatggtg ctggcgcgtc ctttaggcag
     1021 cgggctggcg cggctgatta atgacattcc tcttcccggt acgacgggcg ttgagcgcgt
     1081 actttttcgc gcgcttggcg tctctgaccg cgagatgaac tggaagcagt atctctcagc
     1141 cattcttggc ctgaatatac tggggctggc ggtgctgttt tttatgttgc tcggtcagca
     1201 ctatctgccg cttaatccac agcagttgcc aggactgtcg tgggatctgg cgctgaatac
     1261 cgccgtcagc tttgtcacaa ataccaactg gcaatcttac agcggtgaaa ccacgttgag
     1321 ctatttcagc cagatggcgg gcttaacggt gcaaaacttt ctttctgccg ccagcgggat
     1381 tgcggtgatt tttgccctca tacgtgcatt tacccgccag agcatgagca cactcgggaa
     1441 tgcctgggtc gatctgctac gcatcacgtt atgggtgcta gtccctgtgg cgttgttgat
     1501 cgcattgttt tttattcaac aaggtgcgct gcaaaacttt ctgccctatc aggcggtgaa
     1561 taccgttgaa ggagcgcaac agctgttacc catggggcct gtagcttctc aggaagcgat
     1621 caagatgctc ggcactaacg gcggtggctt ctttaatgcc aactcgtcgc atccgtttga
     1681 aaacccaacc gcactgacca acttcgtgca gatgctggcg atcttcttga tcccaacagc
     1741 gctgtgcttt gcctttggcg aagtggcggg cgatcgccgt caggggcgca tgttgctgtg
     1801 ggcgatgtca gtgattttta tcatctgcac aggcgtggtg atgtgggcag aagttcaggg
     1861 taatccgcat ctgctggcac tgggcgcgga cagcagcatc aatatggaag gtaaagagag
     1921 tcgtttcggc gtgctggtca gtagcctgtt tgcggtcgtg actacggcgg cttcctgtgg
     1981 cgcggtgatt gcgatgtatg attcgtttac cgctcttggt ggcatggtgc cgatgtggct
     2041 gatgcaaatt ggtgaagtgg tgttcggcgg tgtcggttct ggtctttacg gcatgatgct
     2101 gtttgtcctg ctggcggtgt ttattgctgg gctgatgatt ggtcgtacac cggaatatct
     2161 gggtaaaaaa atcgacgtac gcgagatgaa actgaccgcg ctggcgattc tggtcacccc
     2221 aacgctggcg ctgatgggcg cggcgctggc aatgatgacc gacgccggac gtagcgccat
     2281 gctcaaccct ggcccgcatg gttttagcga agtgctgtac gccgtgtcat ccgccgctaa
     2341 caacaacggc agcgcctttg ccggattaag cgccaactct ccattctgga actgtttgct
     2401 ggcgttctgc atgtttgtcg gtcgcttcgg ggtaattatc ccggtgatgg caattgccgg
     2461 ttcgctggtg agtaaaaaga gccagcccgc cagctccggc acgctgccaa cgcacggccc
     2521 gctgtttgtt ggcctgttaa tcggcaccgt gttgctggtt ggcgcactga cctttatccc
     2581 tgccctggcg cttggtccgg tggcggaata tctctcctga tgatattgag tgagcactga
     2641 atatgagtcg taaacaactg gcgctattcg aaccaacact tgtcgttcag gcgctgaaag
     2701 aagcggtgaa aaaattaaac ccgcaggcgc aatggcgtaa tccagtgatg tttatcgtct
     2761 ggatcggcag tctgttaacc acctttatta gcattgcaat ggcaagcggc gcgatgcctg
     2821 gcaatgcgct gtttagcgcg gcaattagcg gctggctgtg ggtcaccgta ctgttcgcta
     2881 atttcgccga ggcgctggca gaaggccgca gtaaagcgca agccaatagt ctgaaagggg
     2941 tgaaaaaaac tgcctttgcc cgcaagctgc gtgagccgaa atatggcgct gcggcggaca
     3001 aagttcccgc agatcaactg cgtaaaggcg atatcgtact ggtagaagct ggcgatatta
     3061 tcccctgcga tggtgaagtc attgaaggcg gcgcatcggt cgatgaaagc gccatcaccg
     3121 gggaatcggc tccggtgatc cgtgaatccg gcggcgattt tgcctccgtc actggcggca
     3181 cgcgtattct ttctgactgg ctggtgattg agtgcagcgt taaccccggc gagacatttc
     3241 tggatcggat gatcgcgatg gtggaaggcg cgcagcgacg caaaacgccg aacgagattg
     3301 ccctgaccat cctgctgatt gccctgacta tcgtcttttt actggcaacc gccacgctgt
     3361 ggccgttttc cgcatggggt ggtaatgcag tcagcgtaac ggtactggtg gcgctgttgg
     3421 tctgtctgat cccaaccact attggcggcc tgttgtcagc gatcggcgtc gccgggatga
     3481 gccggatgtt gggcgcgaat gtgattgcca ccagcgggcg tgcagttgaa gcggcgggcg
     3541 acgtggacgt cctgctgctg gataaaactg gcaccatcac cctcggtaac cgtcaggcat
     3601 cagagtttat ccctgcgcag ggcgtagagg aaaaaacgct ggccgacgca gcacaactgg
     3661 cttcgctggc cgacgaaacg ccggaaggcc gcagtattgt gatcctcgcc aagcagcgct
     3721 ttaacctgcg cgagcgcgat gtgcagtcgc ttcacgccac ttttgtaccg tttactgcgc
     3781 aaagtcgaat gagcgggatc aatatcgaca accgcatgat ccgtaaaggt tctgtcgatg
     3841 ccattcgccg ccatgttgag gctaacggtg gtcacttccc tgtcgatgtt gatcaaaaag
     3901 tcgatcaggt tgcgcgtcag ggggccacgc cgctggtggt ggtggaaggt tctcgtgtgc
     3961 tgggcgttat tgcgctgaaa gatatcgtca aaggcggtat taaagagcgc ttcgcccagc
     4021 tgcgcaaaat gggcattaaa acggtgatga ttaccggcga taaccgtctg actgccgccg
     4081 ccattgctgc ggaagcgggt gtcgatgatt ttctcgccga agcgacaccg gaggccaagc
     4141 tggcgttaat tcgtcagtat caggcggaag gccgtttggt ggcgatgacc ggcgacggca
     4201 ccaatgatgc tccggcgctg gcgcaggcag atgtcgcggt agcgatgaac tccggtactc
     4261 aggcggcgaa agaagcgggc aatatggtgg atctcgactc taacccgacc aagttgatcg
     4321 aggtggtgca tattggcaaa cagatgctga tgacccgtgg ctcgctgacc accttcagca
     4381 ttgccaacga tgtggcgaaa tacttcgcca ttattccggc ggcgttcgcg gcaacgtatc
     4441 cgcagttaaa tgcgctgaac atcatgcgcc tgcattcgcc cgactccgcc attctcagtg
     4501 cggtcatttt caacgccttg attatcgtct ttttgattcc cctggcgtta aaaggcgtga
     4561 gttataaacc gcttaccgct tctgccatgt tgcgccgtaa cttatggatt tacggtctgg
     4621 gtgggctgct ggtgccgttt attggtatca aagtcattga tttactgctg accgtttgcg
     4681 gtctggtgtg aggtttacca tgcgtggatt acgtccggca ttatcaacat ttctcttttt
     4741 gttactgatt actggcggtg tttacccgct gctgaccacc gcgctggggc aatggtggtt
     4801 tccctggcag gccaacggtt cgttgattcg cgaaggtgat acggtgcgcg gctcggcatt
     4861 gatcgggcag aattttactg gcaacggcta ttttcatggt cgcccgtcgg caacggcaga
     4921 aatgccctat aatccacagg cttctggcgg gagcaatctg gcggttagta acccggagct
     4981 ggataaacaa atcgctgcac gcgttgccgc attacgggcc gctaacccgg atgccagcgc
     5041 gaacgttccg gttgaactgg tgacggcgtc ggcaagcggg ctggacaata acatcacccc
     5101 gcaagcggcg gcctggcaga tcccacgcgt ggcgaaagcg cgtaatctca gcgttgaaca
     5161 gctcacgcag ctgatcgcaa aatatagcca acaaccgctg gtgaaatata tcggccagcc
     5221 ggtcgtcaac attgtcgaac tcaatctggc gctggataag cttgatgaat aacgaaccct
     5281 tacgtcccga ccccgatcgt ctgctggaac aaactgccgc gccgcatcgg gggaagctga
     5341 aagttttctt cggtgcctgc gcaggcgtcg gcaagacctg ggcaatgctg gcagaagccc
     5401 agcgactgcg ggcgcaaggg ctggatattg tggttggcgt ggtagaaacc cacgggcgga
     5461 aagataccgc cgcaatgctg gaagggctgg ctgttctgcc gttaaaacgc caggcgtacc
     5521 gtgggcggca catcagcgag tttgatctcg atgccgccct cgcccgccgt ccggcactga
     5581 tcttaatgga cgaactggcg cacagtaatg cgccaggttc tcgtcatccc aaacgctggc
     5641 aggacatcga agagctgctg gaagctggca ttgatgtttt cactaccgtc aacgttcaac
     5701 atctggaaag cctgaatgat gtggtcagcg gcgtcaccgg aattcaggtt cgggaaaccg
     5761 tgcccgatcc tttttttgat gccgccgacg acgtagtgct ggtggacttg cccccggacg
     5821 atctgcgcca gcgactgaaa gaaggcaaag tctatattgc cgggcaggcg gagcgcgcca
     5881 ttgaacattt tttccgcaaa ggcaatctga tcgccctgcg cgaactggca ctgcgccgta
     5941 ctgccgatcg cgtagatgag caaatgcgcg cctagcgggg gcatcctggc gaagagaaag
     6001 tctggcacac gcgcgacgcg atccttttat gcatcggcca taacactggt agtgagaaac
     6061 tggtccgcgc ggcggcgcgg ctggcgtcac ggctgggtag cgtctggcac gcggtgtatg
     6121 ttgaaacccc tgccctgcat cgcttaccgg agaaaaaacg tcgggcaatt ctcagcgcct
     6181 tacgtctggc gcaggagctg ggcgcggaga cggcaacact ttctgatcca gcggaagaga
     6241 aagcggtagt gcgttacgcc cgtgagcata atctcggcaa gattattctc ggtcgcccag
     6301 cctcgcgccg ctggtggcgt cgggaaacgt ttgctgaccg actggcgcgc atcgcccccg
     6361 atctcgatca ggtgctggtc gcgcttgatg aaccacccgc ccgcacgatt aacaacgcgc
     6421 cggatagccg ctcttttaaa gacaagtggc gggtacagat tcagggatgc gtggttgccg
     6481 ccgcgttatg cgccgttatc accttaattg ccatgcagtg gctgatggcg tttgatgccg
     6541 ccaacctggt gatgctgtat ctacttggcg tggtggtggt ggcgctattt tatggacgct
     6601 ggccttcggt ggttgctacc gtcattaatg tggtgagttt cgatctcttt tttatcgccc
     6661 cacgcggcac gctcgccgtc tctgatgtgc aatatctgct gaccttcgcg gtgatgttaa
     6721 ccgtcgggct ggtgatcggg aaccttactg ctggcgtgcg ttatcaggcg cgggtagccc
     6781 gttaccgcga acaacgcaca cggcacttat atgaaatgtc caaagctctg gctgtgggcc
     6841 gcagttcgca ggatatcgcc gccaccagcg aacagtttat tgcctccacg tttcatgccc
     6901 gtagccaggt gttgctgcct gatgacaacg gtaaattgca gccgttaaca catccgcaag
     6961 gaatgacgcc gtgggacgat gccatcgcgc agtggagttt tgataaaggt ctgcctgcgg
     7021 gcgcgggcac cgacacgtta cccggtgtac cgtaccagat tttgccgcta aaaagcggcg
     7081 agaaaaccta cggactggtg gtggtggaac cggggaatct gcgccagttg atgatcccgg
     7141 aacagcagcg cctgctggag acgtttacgc tgttagtcgc caatgcgctt gagcggctga
     7201 cgctgaccgc cagcgaagaa caggcgcgga tggcaagcga acgtgaacag atccgcaacg
     7261 ctctgctggc ggcgctttcg catgatttac gcacgccgct cacggtgctg ttcggtcagg
     7321 cagaaatctt aacgctcgat ctggcaagcg aaggatcgcc ccacgcccgc caggccagcg
     7381 agattcgtca gcatgtgctg aacacgaccc gactggtgaa taatctgctc gatatggcgc
     7441 gaattcagtc cggcggcttt aatttgaaga aagagtggtt aacgctggaa gaagtggtcg
     7501 gcagcgcgct gcaaatgctg gaaccgggtt tatcgtcgcc tatcaatctt tctctgccag
     7561 aaccgctgac cttaatccac gttgacgggc cgctctttga acgggtgctg attaatctgc
     7621 tggagaacgc ggtgaaatac gcgggtgcgc aggccgaaat tggtatcgat gcccacgttg
     7681 agggcgagaa gctacaactg gatgtctggg ataacggccc cggtcttccg ccaggccagg
     7741 agcagacgat atttgataag tttgctcgcg ggaataaaga gtcggcggta ccgggtgtag
     7801 ggcttgggct ggcaatttgt cgggcgattg tggatgtaca cggcggcacc attaccgcgt
     7861 tcaaccgacc ggaaggtggt gcctgttttc gtgttacact tccccagcaa accgctcctg
     7921 aacttgaaga atttcatgag gatatgtgac aaacgttctg attgttgaag atgaacaggc
     7981 tattcgtcgc tttctgcgca cggcgctgga aggcgacggt atgcgcgtct ttgaggccga
     8041 aacgctgcaa cgcggcttgc tggaagcggc tacccgtaag ccagatttga ttattctcga
     8101 tctcggcctg cccgatggtg atgggattga gtttatccgc gacctgcgcc agtggagcgc
     8161 ggtgccggtg attgtgcttt ccgcacgtag cgaagagagc gacaaaatcg ccgcgctgga
     8221 tgccggagcg gatgattatc tgagtaagcc gtttggcatt ggcgaattgc aggcccgtct
     8281 gcgcgtcgct ttacgtcgcc actccgccac cgccgcgccc gatccgctgg tgaaattttc
     8341 cgatgttacc gtcgattcag cagctcgtgt gatccatcgg ggcgaggaag aggtgcatct
     8401 caccccgatt gagttccgcc tgctggcagt gttgcttaat aatgcgggaa aagtgctcac
     8461 ccagcgccag ctccttaacc aggtgtgggg gccaaacgcg gtcgaacaca gtcattattt
     8521 gcgtatttat atggggcatc tgcgacaaaa actggaacag gacccggccc ggcctcgcca
     8581 tttcattact gaaaccggta ttgggtatcg gtttatgctt tgaatattaa tttcaataca
     8641 tcctgccttt tattaattaa agccgtaata ataaatacgg ctttttatct taaacaacac
     8701 acaaaaataa caattcaata ttttatatta ctgagtaaaa agctcatcat taaataaatt
     8761 aagattgatc attttttatt gatcaccttc acagttcaac cgtatttcct ggatagaata
     8821 ccttcacctt cattcacatc aggaaaggta aattaaatgg aaaataacag ccgcactatg
     8881 ccccatataa ggcggacaac tcatattatg aagtttgctc atcgcaatag cttcgacttt
     8941 cacttcttca atgcccgtta gtctaccgac taagggcact tcagcgtaca ggtcttcctg
     9001 actctctgta ttacaggcat atgcctgtat tccgcagtgc tcattctctg ttaacccttg
     9061 tattatcagc caccggactg ataagggttt ctgcattctg aaaaaagcaa acttctgatt
     9121 aatttatcag cggggtaact ttgctttttt tccggcacga tcgatttctc attcgagaaa
     9181 ttgaggacct gctattaccc aaaataaaga gatggaaaat gtcaaaatta aaaattgcgg
     9241 ttagtgattc ttgcccggac tgttttacca cgcagcgaga atgtatctat attaatgaaa
     9301 acagtaatat cgatgtggcg gcaatagttt tatcgctcaa cgatgttaca cgcggaaaac
     9361 tcgatgaaat cgatgccacg ggttatggca tcccggtatt tattgctact gaaaatcaag
     9421 aacgtgtacc cgcagagtat ttgccccgta tttcgggtgt ctttgagaat tgcgaatcgc
     9481 gacgagaatt ttatggtcgt cagttagaaa ccgcagccag ccattatgaa acgcaactgc
     9541 gcccgccctt cttccgcgca ctggtcgatt atgtcaatca gggtaacagc gcgtttgatt
     9601 gccccggtca tcagggcggc gaatttttcc gtcgccatcc cgcggggaat cagtttgtgg
     9661 aatactttgg tgaggcgctg ttccgtgctg acttgtgcaa cgccgacgta gcgatgggcg
     9721 atctgctgat tcacgaaggc gcgccatgca ttgcacagca acatgcggca aaagtgttta
     9781 atgccgataa aacatacttc gttttaaatg gcacttcatc ttctaacaaa gtggttttaa
     9841 acgccctgct gacaccgggt gatctggtgc tgtttgaccg caataaccac aaatctaacc
     9901 accacggagc gttgctacag gctggcgcga caccggttta tctggaaacc gcacgtaacc
     9961 cgtatggttt tatcggtggt attgacgctc actgttttga agaaagttac ctgcgtgaac
    10021 tgatcgcgga attcgcaccg cagcgggcaa aagaggctcg tcctttccgc ctcgccgtta
    10081 ttcagctagg cacttacgac ggtacgattt ataacgcccg tcaggtggtg gataaaattg
    10141 gtcatctgtg tgactacatc ctctttgact ccgcatgggt cggctatgag cagtttatcc
    10201 cgatgatggc ggactgttca ccactgttgc tggatcttaa tgagaacgat ccgggtattc
    10261 tggttacgca atctgtgcat aaacagcagg ctggtttttc ccagacttca caaatccata
    10321 aaaaagacag ccatatcaaa gggcaacagc gttatgtacc gcacaaacgc atgaacaacg
    10381 cctttatgat gcatgcctcc accagcccgt tctatccgct gtttgccgca ctggatatca
    10441 acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg
    10501 gcattaatgc ccgcaaacta atcctcgata actgtcagca tattcgtcca ttcgtacctg
    10561 aactggtgga tggtaaaccc tggcagtcgt atgaaacggc gcaaattgcg gttgatctgc
    10621 gcttcttcca gtttgtacca ggtgagcact ggcattcttt tgaaggctat gcagagaatc
    10681 aatactttgt cgatccgtgc aaactgttgc tgacaacccc aggtattgat gcacgtaacg
    10741 gcgaatatga agcgttcggt gtacccgcga cgattcttgc taacttcctg cgcgaaaatg
    10801 gcgtagtgcc ggaaaaatgc gatcttaact ccatcctctt tctgctgact ccggcagaag
    10861 atatggccaa acttcagcaa cttgttgctc tgctggtacg cttcgaaaaa ctgcttgagt
    10921 ccgacgcgcc attatcagaa gtactgcctt ccatctacaa acagcatgaa gagcgctaca
    10981 ccggttatac cctgcgtcag ttgtgtcagg aaatgcatga tttgtatgcc cgtcacaacg
    11041 tgaaacaact gcaaaaagag atgttccgta aggaacactt cccacgcgta agcatgaatc
    11101 cacaagaagc caactacgcc tatttacgcg gtgaagtgga gctggttcgt ctgccggatg
    11161 cagaaggccg tatcgctgcc gaaggtgcgc ttccttatcc tccgggtgtg ctgtgtgttg
    11221 ttccgggtga aatctggggt ggtgctgttc tgcgttactt cagcgctctg gaagaaggga
    11281 tcaacctgct gccaggtttt gcaccagagc tgcagggtgt ctatatcgaa gaacatgatg
    11341 gtcgtaagca agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc accctgttga
    11401 aaggggaaaa attatgagtc aggctaaatc gaataagatg ggcgtcgttc agttaaccat
    11461 actgacgatg gtcaacatga tgggctccgg tatcatcatg ctgccgacaa agcttgccga
    11521 agttgggaca atctcaatta tctcctggct ggtgacagcc gtaggctcaa tggcactggc
    11581 atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca ggcggtatgg gcggttatgc
    11641 ggagtatgct ttcggtaaat ccgggaactt tatggcgaac tacacctacg gtgtctcgct
    11701 gctgattgct aacgtcgcga ttgctatttc ggcggttggt tacggcaccg aactgctcgg
    11761 cgcgagtttg tcgccagtgc agattggtct tgcgaccatc ggagtgctgt ggatttgtac
    11821 cgtggctaac tttggtggtg cgcgcattac cgggcaaatc agtagcatta ctgtgtgggg
    11881 ggtcattatt ccggtcgtcg gtctgtgcat tatcggctgg ttctggttta gcccgacgct
    11941 atacgctaac tcatggaacc cgcaccatgt gccgttcttt agcgctgtag gttcttccat
    12001 cgccatgacg ttgtgggctt tcctcggtct ggagtcagcc tgtgccaacg ccgaagtagt
    12061 tgaaaacccg gaacgtaacg tgccgattgc agttcttggc ggtacgttag gtgcggcggt
    12121 gatttatatc gtctccacca acgtgattgc cgggattgtg ccaaatatgg agctggcaaa
    12181 ttcaacggca ccatttggtc tggccttcgc gcagatgttc acgccggaag tgggtaaagt
    12241 cattatggcg ctgatggtga tgtcctgctg cggttcgctg cttggctggc agttcaccat
    12301 tgcccaggtg tttaaatctt ctgccgatga agggtacttc ccgaaaattt tctcccgtgt
    12361 gagcaaagtc gatgcaccag tacagggaat gctgaccatt gtcatcatcc agagcggatt
    12421 atcactgatg accattagcc cgtcgctgaa cagtcagttc aacgtgctgg ttaacctggc
    12481 cgtggtgacc aatatcattc cgtatattct gtcaatggcg gcattagtca ttattcagaa
    12541 ggtcgctaat gtgcccccct caaaagcgaa agttgcaaac tttgttgctt ttgttggcgc
    12601 gatgtacagc ttttatgcgc tgtactcatc cggggaagaa gccatgctat acggttccat
    12661 cgtgaccttc ctcggttgga cactgtatgg tctggtctca ccacgctttg aactgaaaaa
    12721 taaacacggt taattaagca aagcaaaaaa gggcgatctt ccgaccgccc tttttcttat
    12781 ttgatatgtt tacgcgtttt tcagaacttc gctaacaatc tcaaccgctt ctttctcaat
    12841 ctgcttgcga tgttcttcac caaggaagct ttcgcagtag atcttatatg cgtcttccgt
    12901 gcctgacgga cgcgcggcga accagccgtt gtcagtcatc actttcagac cgccgataga
    12961 agcgccatta cccggtgccg ccgtcagacg cgcggtgatc gggtcacctg ccagggtgct
    13021 ggcgctcacc atttccggag acagcttaga caacgccgct ttttgtgcgg aagtcgctgc
    13081 tgcctgcaaa cggttatagc tcggcgcacc aaagcgtttt gccagttcgt tatagtgttc
    13141 ctgcgggttc ttaccggtga cagcggtgat ttccgccgcc agcagacaca taatgatacc
    13201 gtctttatcg gtggaccacg gcgtgccgtc gaaacgcagg aaggaagcgc ctgcactctc
    13261 ttcgccgcca aagccgaagc tgccatcgaa caaaccatca acaaaccatt tgaaacctac
    13321 cggtacttct accagcttac ggcccaaatc attgaccaca cggtcgatca tcgcagatga
    13381 aaccagcgtt ttaccgacag caacatcttt gccccactgc ggacgatgct ggaacaggta
    13441 gttgatcgcc accgccaggt agtggttcgg attcatcaaa cctgccggag tgacgatacc
    13501 gtgacggtca taatccgggt cgttagcaaa cgccagatcg aacttatcac gcagtgccag
    13561 caggcccgcc atcgcacact cggaggagca gtccatacgg atcgcaccgt ctttatcgag
    13621 gtgcataaag cggaaggttt gatcgacctg atcgttaacg atggtcaggt tgaggttgta
    13681 atactcgcca atgcgcttcc agtattcgat accggaacca cccagcggat caacgcccag
    13741 cgtcaggccc gctttctgaa tcgccgccat atcaacgata tcggccagcc cttccacaaa
    13801 cggctgcacc agatcctgct ctttcacatg accggatgcc atcgcttcgt cgagggagat
    13861 acgcttcacg cctttcaggc catcagccag cagtgcgttg gctctgtctt ccaccacttt
    13921 agtgacgttg gtatcagccg ggccaccatt tggcggattg tacttgatac caccatcttc
    13981 cggcgggtta tgggacggtg taatcacgat accgtctgcc agtgggccac cttttttatt
    14041 gtgaaccaga atggcattgg aaatggcagg tgtcggagtg aagccattgt tttcctgcac
    14101 aatgacatca acgccgttcg ctgccagcac ttccagtacg gaaatgaacg caggctcgga
    14161 cagggcgtga gtatctttac ccacatagca agggccagtg atgccgtttt tcgcacgttc
    14221 ttcagcaatt gcctgagcga tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc
    14281 actgccacgg tgaccggaag taccgaattt caccgcgtgc tccgcattcc ctgcttctgg
    14341 tttcagtaca taatattgcg ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc
    14401 aggttggcct gcacgattgt ggattgccat tgctttgtcc tttgtctgca acgttttaga
    14461 tagttccgca aaccttctca atcaattccg ccgggaattg catcgactgc atgatgtgtt
    14521 cgatcatgct gcatttacgg ccggtgttgg tgttggtgat cacccaatac ggcgtaccag
    14581 gcacatgttt cggcttggtc tgattaccat ttttcagcag cgtttgttca tctgccgcaa
    14641 agtaaacgcg tgtacgaccg tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa
    14701 gagaatatag tgtagacaac agcagcataa agcgattgac cgctcgcttt tgctctgcgt
    14761 attcgtccga aagcagaagt tcacgcattg cgcgaacctt gtctttaatc gttttgaccg
    14821 gcttcgcttc gacgatagca ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag
    14881 gctgtgatgc ggcggaaaat ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga
    14941 tatgcttagt gtggctggca atatagctgt agagttcatc atcaacttca atcgttttca
    15001 tcttaatcca gtgcagtgtc ttagctgaat acaagtcgcc aggagtatat gaataaatcc
    15061 cggcagcgga tagcgtcaag cctggcttgc agaaaaactc ggaataaacc ttatttgttg
    15121 cagccctgtg gcacaatggt caacataata ccctaacccg acaacgcgaa aaaaagaact
    15181 ttgccatgaa attgaatatc cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca
    15241 tcgttcttgt ccatggtctg tttggcagcc tcgacaacct tggcgtactg gctcgcgatc
    15301 tggtaaacga tcacaatatc atccaggttg atatgcgtaa ccacggtctt tcaccgagag
    15361 atccggtaat gaattacccg gcgatggcgc aggatcttgt tgataccctg gatgcacagc
    15421 agatcgacaa agcaacattt atcggtcact ccatgggcgg taaagcggta atggctctta
    15481 ctgcactagc ccccgatcgc attgacaagc tggtggcgat tgatattgcg ccggtcgatt
    15541 atcacgttcg ccgtcatgat gagattttcg cggccatcaa cgcagtcagt gaatcagacg
    15601 cacaaacacg ccagcaagca gcggcaataa tgcgccagca tcttaatgaa gaaggggtga
    15661 ttcagtttct gttgaaatct tttgttgacg gggagtggcg ctttaacgtg ccagtattgt
    15721 gggatcagta tccgcatatt gtgggttggg agaaaatccc ggcatgggat catcctaccc
    15781 tgtttatccc tggcggcaat tctccgtatg ttagcgagca gtaccgtgat gatttactgg
    15841 cacaatttcc acaggctcgg gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg
    15901 aaaaaccgga tgcggtatta cgcgctatcc gtcgctatct caatgattaa cgcaactgat
    15961 taaaaaccca gcgtccgcgt cgctttccgc gcgtggacgt tggcgtgact catcgcgctg
    16021 atgtattatg gcgcgctatc catccgggca ggcgcctggc tgattgtttc ccccgaagtc
    16081 accaagatca tggccaaaga acaaacggac cgtacgacat tagatctgtt cgcgcacgag
    16141 cgtcgaccgg gacgaccgaa aactaatccg ctttcgcgcg atgagcagct gcgtattaat
    16201 aaacgcaacc aactaaaacg cgacaaagta cgtggcctta agcgtgtcga actgaagctg
    16261 aacgcggaag ctgtcgaggc actgaacgag ctggcggaat cgcgcaatat gagccgtagc
    16321 gagctgatcg aagagatgtt aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt
    16381 taaatttcca ctttcatgta gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga
    16441 ctgcctgttc tgctatgatt gcctttatcc gtgggcaatt ttccaccccc atttcaataa
    16501 gtttcaagag gttatttcac tcatggctat cactggcatc tttttcggca gcgacaccgg
    16561 taataccgaa aatatcgcaa aaatgattca aaaacagctt ggtaaagacg ttgccgatgt
    16621 ccatgacatt gcaaaaagca gcaaagaaga tctggaagca tatgacattc tgctgctggg
    16681 catcccaacc tggtattacg gcgaagcgca gtgtgactgg gatgacttct tcccgactct
    16741 cgaagagatt gatttcaatg gtaaactggt tgcgctgttt ggttgtggcg accaggaaga
    16801 ttacgcagaa tacttctgcg atgcattagg caccatccgc gacatcattg aaccgcgcgg
    16861 cgcaactatc gttggtcact ggccaaccgc gggctaccac ttcgaagcgt caaaaggtct
    16921 ggctgatgat gaccactttg tcggtctggc tatcgacgaa gatcgtcagc cggaactgac
    16981 tgctgaacgt gtagaaaaat gggttaaaca aatttctgaa gagctgcatc tcgacgaaat
    17041 tctcaatgcc tgatgtgatg cggcgtagac tcatgtctac gccgtattaa tagataatgc
    17101 caatcaaaat aattgctaca aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg
    17161 tgttcaagtg gccttgccgt tgtaaatgta agctgtgcca cgtttttatt aacaatattt
    17221 gccagggact tgtggttttc atttaggcgt ggcaattcta taatgatacg cattatctca
    17281 agagcaaatt ctgtcacttc tcctaatgaa gtgaaccgct tagtaacagg acagattccg
    17341 catgactgat aacaataccg ccctaaagaa agctggcctg aaagtaacgc ttcctcgttt
    17401 aaaaatcctg gaagttcttc aggagccgga caaccatcac gtcagtgcgg aagatttata
    17461 caaacgtctg atcgatatgg gtgaagaaat tggtctggct acggtatatc gcgtactgaa
    17521 ccagtttgac gatgctggta tcgtcacccg ccacaatttt gaaggcggta aatccgtatt
    17581 tgaactgaca cagcaacatc accacgatca cctgatctgc ctcgactgcg gcaaggttat
    17641 cgaatttagt gatgattcca tcgaagcgcg tcagcgtgaa atcgccgcaa aacatggcat
    17701 tcgcctgact aaccacagtc tctatcttta cggtcactgt gccgaaggcg attgccgcga
    17761 agatgagcac gcgcacgaag gcaaataagc cagcctgaac gagaaaagcc aacctgcagg
    17821 ttggcttttt tatacaaggg aaataagaaa agcgagagtt acagctctca cttatttgtt
    17881 attactgcga atttcctgcc agaccttatc acaatcggct ttcaccgcct gatcatttcc
    17941 ggtttgcgtt gcctgaatac actgctgata atccagtacg cggacacttt cctgctcggc
    18001 aaactgctga tgttgtttct ctttcttcag cacgtttaac acgctctggc aggcttcaat
    18061 tttttccggc gaaccttgcg cggtgttgat acaggcgctg taagcctctt tcagacgact
    18121 gtcttcctgc ggtgcggttg attgcgcaca agctaccagc cccgatgcca tcatggcgat
    18181 gagaatcagt tttttcatag caaattcctc aaatgtgcgg caggcacaaa acctgccgcg
    18241 tcgggcatca gaagatggtg aatggtgcga ttaccataaa tttcacgtcg cgctcatcct
    18301 ggaagatgtt accgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg
    18361 tgaagtgcag tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tacaccgcat
    18421 ccaggctgta ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc
    18481 tctgccaggt cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc
    18541 ctggaagatt ccagtttttc aggtcataca tcgcaccgaa gaagaccgct ttttcgccgt
    18601 tggcgttgaa gtcagaacgg ttatcccacc agatatccag gcgaccgttc gaggaagcgt
    18661 aggttggagt catacgttgc aggaagtatc cctgctgacc gtcggcttta acccagctgc
    18721 cttccagacg caaatccact acatcagccg cccggtaacc aaaggtcaac gcttgcagcc
    18781 aggcagtgcc gtcgtaaatg tcgttgacgc tgcgatcgtc aactttatcg cgcgtaccgt
    18841 agaactggta gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg
    18901 caaaatattg atctatatac ccttccgcct gaccaaatgc cgcttccagt acaaagttat
    18961 ttttgaagtc gtacttcgca ccgatggagt gcagataatc aactttggta gttttatcgt
    19021 tctgataaaa ctcatccatt tcgagatgcc acggtgcttt gtattcgttg gtccacatgt
    19081 aggagaaact caacgcacca gcatcgccgt aatcaaaatt cgccccggct tccgcaccct
    19141 gataagtacc cggcataaag ctccagtgcg gcgctaacag cgtctgacca gttggctgaa
    19201 tgtaacctgc cctcgcccac accggaccgt atttaaattt ggccgctgct ttatacaggc
    19261 ttataccgct tttgtcgccg gaccagtctt cgtcataggc tttattgctt tttgaaaacg
    19321 cgatttcgtt cgggtgagag ctgtcaccgt tttccgccat ttcaatcgcc gtaaacgcag
    19381 caatatcaag gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc
    19441 aggtggagtg agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc
    19501 gttcacgctg ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg
    19561 cgcgagcctc cggcattgcc ataaagcccg acattgctgt aacaccggcg atagccagcg
    19621 ccagcgtact acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat
    19681 tgctgcacca aaggtgaaca gcgaaaaaaa taaaaattaa aaaagtagcg ttgagaaaag
    19741 tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc tctttctgta
    19801 accagaatgt aagaacatga gcaaccgcac atattttgat gatttttgtt acaaggctta
    19861 agtttgcagt tgaaaggctt gcagaagaat aaaagcgtga atttccagta agatgaaaac
    19921 ggttacattt tatttaatta tggtttgtga ttttaaattt gttttatgga taaattgcga
    19981 agcggaatta attcgcgaag aaaagcagaa aaaaacgccg cagagcgcgg cgtttgggat
    20041 tgtttgcata tttcttgccg gatgtggcgt aaatgcctca cccggcctgg tagaacttaa
    20101 aactactcgc ctactttcgc ccaggtatca cgcagcccta cggtgcggtt aaataccggt
    20161 ttttcggcag tggaatggcg gctgtcgagg cagaaataac cttcacgctc aaactggaat
    20221 gctttacccg ctaccgcgtc tttcagcgac ggttcagcga aaccctgttt gatcaccagc
    20281 gattccgggt taatcaccga caggaaatca tccgcagcac ctgggttagg cacgctgaac
    20341 agacggtcat acaaacggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata
    20401 acacctttca ctttacgacc atctgccgga tctttgctta aggtgtcggc gtcataagta
    20461 cagaagatgg tggtgatatt accttcggca tctttctcga cgcgttctgc cttaatcacg
    20521 taagcattac gcagacgcac ttctttaccc aacaccagac gtttgtactg cttgttagct
    20581 tcttcgcgga aatcggcgcg atcgatccaa atctcacagc taaacggcac ctgacggcta
    20641 cccatttccg gtttgttcgg atggttcggc atggtgacca tttcgccttc gccctggtag
    20701 ttttcgataa ccagtttcac cggatcgata accgccattg cgcgcggggc attttcgttg
    20761 agatcttcac ggatgcagga ttccagtgac gccatctcaa tggtgttgtc ctgcttggtc
    20821 acaccaatgc gtttgcagaa ctcacgaata gaagccgcag tgtaaccacg acgacgcaga
    20881 ccagaaatgg tcggcatacg cgggtcatcc cagccttcaa cgtgcttatc ggtcaccagc
    20941 aggttcagct tacgcttgga catcacagtg tattccagat tcaggcgcga gaattcgtac
    21001 tggcgcgggt gaacaggaat ggtgatgttg tccagtaccc agtcgtacag acggcggttg
    21061 tcctggaact caagcgtaca cagagagtgc gtaatacctt ccagcgcatc gctgatgcag
    21121 tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca
    21181 aatttaatgc ggtacagcac cggatcgcgc attacgataa atggcgacgc catgtcgatt
    21241 ttcgcacgca ggcaggcttt accttcttca aaactaccgg tacgcatttt ttcgaacagc
    21301 gccaggttct cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg ctgcgtcagg
    21361 gtgccgcggt attcgcggat ctgttccggt gtcagttcat caacgtacgc caggccttta
    21421 ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat aatcggagga gtaacggacg
    21481 ttaccagacc agtgaaaacc taaccactca acgtcgtttt tgatcgactc aacatactcg
    21541 atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc
    21601 tgggcgatcc cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc
    21661 ggctccggcg ggaaacgggt gtgtactgtg gtgtgcttac cactggccag atcttcatcg
    21721 atgatctgac gaataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca
    21781 aagcgtaaac aacgtataac ggcgtatgat cttataagcg ggacaggctg acaactgtta
    21841 gttagtgaaa atcgaaaatc tcactgagat tatggggatg attgatgaaa aaaaaagcgg
    21901 cagagaagat ctccaccgct tgtcattgtt ggatgcgacg ctcaagcgtc gcatcaggca
    21961 taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa catggccctg
    22021 agctttaatg atcaggccgc tgaaatcgtc gatattgctg caaaccaccg ggctaatcat
    22081 cgagcgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt gtcctgcgct
    22141 tacctgcgcg ccctcttcca ccagacgttt aaagccttta ccttccagcg ctacggtgtc
    22201 gatacccata tggacgacga tctccgcgcc tttttcggtt tccagacaga acgcgtggtt
    22261 ggtgttgaag attttcacga ttgtccctgc ggctggtgat acgacgattt tatctgtcgg
    22321 tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc
    22381 cagtgccaca acatcaccgg taatcggcga taccagctcc gcgatagaca ccgcgtttgg
    22441 tacagcctgc ggttttgcta caggcgcggc agttgcaggc gcagtttcag ctgacgcagc
    22501 ggctaccgga ccacgggcaa cgactttctt catcgcatcg ccgatggatt ctgctttcgc
    22561 gcccacaatc acctgaatag tctgtttgtt cagttttact accccagaag cccccagacg
    22621 tttacacatc acatcgttga cgcgagctga gtcaaccacg gtaaggcgca gacgagtaat
    22681 acaggcgtca atggctttca ggttgtcagt accgccaacc gcagcaatat agttggttgc
    22741 cagttgattg agaccttctt ccgtgttgct gttggcttct tcagtaacga tctcgtcttc
    22801 tttatcttca cggcccggcg ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac
    22861 gaagtagata gcgaagaaga caacacccat caccagcagc atccagacgt tctggctggc
    22921 ggccggcagg ttatacatca acgcgtagtc gatagccccc gcagagaagg agaagcccgc
    22981 gtggataccc agcagcgttg ccacaaacag gctgataccg gtcagcagtg cgtgcaggag
    23041 gtacagcagc ggcgcaagga acatgaacag gaattccagc ggctcagtca caccggtcag
    23101 gaacgcagta acagcaacag aaagcagcat accgccaacc atcggacgac gctctttcgg
    23161 tgctgcaaag tacatcgcca gcgccgcacc cggcagaccg aacatcatga tcgggaagaa
    23221 gccggacatg aacatccccg cggtgccgtc accggcatag aagcggttaa tgtcgccgtg
    23281 gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga tggtgttcag
    23341 tacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga taccggaacc
    23401 cagcgcgccc gcagaaacga tccactcgcc gcctgcatgg atagcgtgct gtaccggcgg
    23461 ccagacgtaa ccaaaaatgg ccgccagcac caggcagaag aagccggtgg caatcggcac
    23521 aaagcgtttg ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg accaacggtt
    23581 ataggctgca ccaccgacca gaccggtaat gatacccgcc agtacaccca tgttaatttc
    23641 tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac ccgccagtgc
    23701 cgccgcacct gcgctgtctt tcgaccagct ggatgccacg ccgatggcga agattaatgc
    23761 gaggttatca aaaatcgcac cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg
    23821 ctgaccgaat cgcagcaaca gcgccgccac cggcagcacc gcgataggga gctgtaacgc
    23881 cctacctagt cgctggaaaa aacctaaaat attcatctta ttccccctac gagaacccta
    23941 tttggctcgt ttaaagccgt atttttattt tgctgcaaat tgtactgccg atgttctgta
    24001 atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgta tcgcaaatta
    24061 aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc cttttactgg
    24121 ctaaaccaga aaacttattt tatcattcaa aaaatcaggt cggattgacg cctgtctgcg
    24181 caaatccagg ttacgcttaa agatgcctaa tccgccaacg gcttacattt tacttattga
    24241 ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc
    24301 gccatatcgt caatcgtatc aatgcgttca aaccgactgc cgatcgtccg tttgtactgg
    24361 gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa atgcataaag
    24421 caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat gtcggtctgc
    24481 cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc gatcacgttg
    24541 atattccagc agaaaatatc aaccttctca acggcaacgc cccggatatc gacgccgaat
    24601 gccgccagta tgaagaaaaa atccgttctt acggaaaaat tcatctgttt atgggcggtg
    24661 taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct tctcgtactc
    24721 gtatcaaaac cctgactcat gacactcgcg tcgcgaactc tcgtttcttt gataacgatg
    24781 ttaatcaggt gccaaaatat gccctgactg tcggtgttgg tacattgctg gatgccgaag
    24841 aagtgatgat tctggtgctg ggtagccaga aagcactggc actgcaggcc gccgttgaag
    24901 gttgcgtgaa ccacatgtgg accatcagct gtctgcaact gcatccaaaa gcgatcatgg
    24961 tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat ttcaatgaat
    25021 tagaagcaga aaatatcaaa ggtctgtaat tattatccct gccctggctc cttgctcagg
    25081 gcaatatttt ttaaaatcgg gggtcagaat gtatgcatta acccagggcc ggatctttac
    25141 cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga ttaaaagcgt
    25201 ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg gggccattct
    25261 ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtgcagt ttaacgatac
    25321 tgctgaagca gtcagcgtag aaacgctgga aatcatgcag aaagccaatg agaaatcagg
    25381 ctgtactaac tatttgccga cgcttatcac caccagcgac gagctgatga aacagggcgt
    25441 gcgcgttatg cgcgagtacc tggcaaaaca tccgaatcag gcgttaggtc tgcatctgga
    25501 aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg tgcgtaagcc
    25561 tgatgccgcg ctggtcgatt tcctgtgtga gaacgccgac gtgattacca aagtgactct
    25621 ggcaccggaa atggttccag cggaagtcat cagcaaactg gcaaatgccg ggattgtggt
    25681 ttctgctggt cactccaacg cgacgttgaa agaagcgaaa gccggtttcc gcgcggggat
    25741 tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg aaccgggcct
    25801 ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attattgctg atggcctgca
    25861 tgttgattac gccaacattc gtaacgctaa gcgtctgaaa ggcgacaaat tgtgtctggt
    25921 taccgatgcc accgcgccag caggtgccaa cattgaacag ttcatttttg cgggtaaaac
    25981 aatatactac cgtaatggac tttgtgtgga tgagaacggt acgttaagcg gttcatcctt
    26041 aaccatgatt gaaggcgtgc gtaatctggt cgaacattgt ggtatcgcac tggatgaagt
    26101 gctgcgtatg gcgacgctct atccggcacg tgcgattggc gttgagaaac gtctcggcac
    26161 gctcgccgca ggtaaagtag ccaacctgac tgcattcaca cctgatttta aaatcaccag
    26221 gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac accaggcgga
    26281 caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc ggtttatcgc
    26341 ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag tcagcttgcc
    26401 cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat caaagaagtt
    26461 gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga aacccgcaat
    26521 ttccacgcaa tcggcgtacg gcttggtcgt cacgacgcca ccatcacctt gtttgatctc
    26581 agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca gcagacgctg
    26641 gaacatgccc tgctgaatgc cattgctcag tttattgata gctaccagcg caagctacgc
    26701 gagctgatcg cgatttcggt gatcctgcca gggcttgtag acccggacag cggcaaaatt
    26761 cattatatgc cgcatattca ggtagaaaac tgggggctgg ttgaagcact ggaagagcgt
    26821 tttaaagtga cctgttttgt cggccacgat atccgcagtc tggcgctggc agagcactac
    26881 ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg cggaaccggg
    26941 gccgggatta tctctaacgg gcgaattttt atcgggcgca acggcaacgt cggtgaaatt
    27001 ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt tggctgcctg
    27061 gaaactatcg ccgccaatgc tgccattgaa caacgggtgt tgaatctgtt aaagcagggc
    27121 taccagagcc gtgtgccact ggacgactgc accatcaaaa ctatctgcaa agccgcgaac
    27181 aaaggcgata gcctggcctc ggaagtgatt gaatatgtcg gtcgtcatct gggtaaaacc
    27241 atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgctgg agaaatcacc
    27301 gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca ggcgctgaag
    27361 gcgtttcgta ctaatctacc ggtggtacgt tctgagttgg atcaccgctc agcaatcggc
    27421 gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca tttgctggaa
    27481 aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag tccatgacca
    27541 ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc gccgtaccgg
    27601 gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg ttgctgacca
    27661 actatccttc gcagaccggg caagatctgg cgaatcgctt tgccactgcc ggtgtcgatg
    27721 taccggatag cgtgttttat acctccgcga tggcgaccgc cgatttcctg cgtcgccagg
    27781 aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg tacaaagccg
    27841 gtttcactat taccgatgtg aaccctgatt ttgtgattgt tggcgaaacg cgttcctaca
    27901 actgggacat gatgcataaa gcagcctatt tcgtcgctaa cggcgcacgc tttatcgcca
    27961 ccaacccgga cacccacggg cgcggttttt atcccgcctg tggcgcgttg tgtgcaggga
    28021 tcgagaaaat ctccgggcgc aaaccgttct atgttggaaa gccgagtccg tggatcatcc
    28081 gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac ggtgattgtc ggcgataact
    28141 tgcgtaccga tattctggcg ggcttccagg ccggtctgga gacgattctg gtgctttctg
    28201 gtgtttcgtc actcgacgat atcgacagta tgccgttccg cccaagttgg atttacccat
    28261 cggtcgctga aatcgacgtt atctgaatag caaccacgcc tcagggcgtg gtttcttatt
    28321 tttacccatc attaaaatca ccccattcaa taaaacccgt caaaaattat cgatccatca
    28381 ataaatatac ccaaaagccc tccccttttt catcaatcag caatcgccat tacgtttttt
    28441 atttttccac tgccaaatcg cttgcgcacc gtgctgcttt gcggcatttt tttaacaagt
    28501 aaacacaaca cgcagtaaac accaggttaa cggagaaggt tatgtgttca atttttggcg
    28561 tattcgatat caaaacagac gcagttgagc tgcgtaagaa agcactcgag ctgtcacgcc
    28621 tgatgcgtca tcgtggcccg gactggtccg gtatttatgc cagcgataac gccatcctcg
    28681 cccacgaacg tctgtcaatt gttgacgtta acgcgggggc gcaacctctc tacaaccaac
    28741 aaaaaactca tgtgctggca gtaaacggtg aaatctacaa ccaccaggca ttgcgcgctg
    28801 aatatggcga tcgttaccag ttccagaccg ggtctgactg tgaagtgatc ctcgcgctgt
    28861 atcaggaaaa agggccggaa tttctcgacg acttgcaggg catgtttgcc tttgccctgt
    28921 acgacagcga aaaagatgca tatctgattg gtcgcgacca tctggggatc atcccactgt
    28981 atatgggcta tgacgaacac ggtcagctgt atgtggcctc agaaatgaaa gccctcgtgc
    29041 cagtttgccg cacgattaaa gagtttccgg cggggagcta tttgtggagt caggacggcg
    29101 aaatccgttc ttattatcat cgcgactggt tcgactacga tgcggtgaaa gataacgtga
    29161 ccgacaaaaa cgagctgcgt caggcactgg aagattccgt taaaagccat ctgatgtctg
    29221 atgtgcctta cggtgtgctg ctttctggtg gtctggattc ctcaattatt tccgctatca
    29281 ccaagaaata cgccgcccgt cgcgtggaag atcaggaacg ctctgaagcc tggtggccgc
    29341 agttgcactc ctttgctgta ggtctgccgg gctcaccgga tctgaaagca gcccaggaag
    29401 tggcaaacca tctgggcacg gtgcatcacg aaattcactt cactgtacag gaaggtctgg
    29461 atgccatccg cgacgtgatt taccacatcg aaacttatga tgtgaccact attcgcgctt
    29521 caacaccgat gtatttaatg tcgcgtaaga tcaaggcgat gggcattaaa atggtgctgt
    29581 ccggtgaagg ttctgacgaa gtgtttggcg gttatctata cttccacaaa gcgccgaacg
    29641 ctaaagagtt gcatgaagag acggtgcgta aactgctggc cctgcatatg tatgactgcg
    29701 cgcgtgccaa caaagcgatg tcagcctggg gcgtggaagc acgcgttccg ttcctcgaca
    29761 aaaaattcct tgatgtggcg atgcgcatta acccacagga taaaatgtgc ggtaacggca
    29821 aaatggaaaa acacatcctg cgtgaatgtt ttgaagcgta tctgcctgca agcgtggcct
    29881 ggcggcaaaa agagcagttc tccgatggcg tcggttacag ttggatcgac accctgaaag
    29941 aagtggcagc gcagcaggtg tctgatcaac agctggaaac tgcccgcttc cgcttcccgt
    30001 acaacacgcc gacctcaaaa gaagcgtatc tgtaccggga gatctttgaa gaattgttcc
    30061 cgcttccgag cgccgctgag tgcgtgccgg gcggtccttc cgtcgcttgt tcttccgcta
    30121 aagcgattga gtgggatgaa gcgttcaaga aaatggacga tccgtctggt cgcgcggttg
    30181 gcgttcacca gtcggcatat aaataagacg tgtaatattg ccccggagaa atccggggca
    30241 atttcagtta ctggggcgct tgttgattct gatatgcagt aaggaaatgt tccgggtttg
    30301 ccacatcgct gtttgacaac tcattactgt aggccagtat ttcatagatt aatttcccac
    30361 cactattgtt gaattccgaa tttcatcctg tatgacaaaa gaatggctgt agggtgggga
    30421 aaaaacacaa agtaaacaat aattgacgaa tatagcgcca tgctgttcgc aacctaacca
    30481 aacagtcact ttcgagcaat tttccttgaa aaagaggttg acgctgcaag gctctatacg
    30541 cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa aaagatggct acgtagctca
    30601 gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc gtcgtagcca
    30661 ccatcttttt tgcgggagtg gcgaaattgg tagacgcacc agatttaggt tctggcgccg
    30721 caaggtgtgc gagttcaagt ctcgcctccc gcaccattca ccagaaagcg ttgtacggat
    30781 ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg gttcgaatcc
    30841 aggtacccca gccatattct tcgaataagc ggttcaccgc ccggttattg gggtatcgcc
    30901 aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca ggtaccccag
    30961 ccatcgaaga aacaatctgg ctacgtagct cagttggtta gagcacatca ctcataatga
    31021 tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc gaatatgttc
    31081 ggcaaattca aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc ggattctgat
    31141 tccggcattc cgaggttcga atcctcgtac cccagccaat ttattcaaga cgcttacctt
    31201 gtaagtgcac ccagttgggg tatcgccaag cggtaaggca ccggattctg attccggcat
    31261 tccgaggttc gaatcctcgt accccagcca cattaaaaaa gctcgcctcg gcgagctttt
    31321 tgcttttctg cgtatattca atgccgaatg cgatgttgac tcgtcttatc cttcaaagtc
    31381 ggatgcgacg ctgccgcgtc ttatccgacc tacggttggc atgcatccag caatgttgtg
    31441 aggctacaac cctaacgcat atttcagcgc cttacgtttc aacacgccag cacgctccgc
    31501 cgccatcaaa ccaagattac gcacaaaacg cagcggtggc agattattgc tgaatccggc
    31561 ataaaacaga tccataccac tttgcatcat aaagttatcc gccatacgcc gcatctggta
    31621 acgcttgagc acaggataac tggcccacgc ttcgccgtag ctgcgggcgt tcaccagaac
    31681 gtcaatcagg gcatcgacat cacgataacc aagattcacc ccctgccccg ccagcggatg
    31741 gatggtatgc gcggcatcgc ccaccagagc aagccctggt tgcacatact gcaacgcatg
    31801 gcgacgtatc agcggaaacg caccagcggc aagcggtgtc acgtaaccca gacgcgacgg
    31861 gaaatgcttc gcgatttccg cctgtagctg cgccatattc atattctgca actggcgaat
    31921 gcgcgccgga gagtcatacc acaccagcga tgcccagtta tcaaacaacg gcagaaacgc
    31981 acgcggtccg tccggggtaa attgctgcca ggtgctgtcg ccgggatcgt tctcacactg
    32041 tacgctaatc aacatacacg actgcgcata ctgccaggca tgaacgccaa ttcccgccat
    32101 ctgtcgcacc tgcgaatttg cgccgtcggc accaatcacc agcttcgcga ggatcgtttc
    32161 accgcccttc agttccagct cctgaagatc attatggcga tgcagtgcaa tcagcgaggt
    32221 tggcacacgt aacgttactt tcggatgcgc ttccagcgcc tgccacaacg cctgttgcag
    32281 gacagtgttt tccaccatgt agccaagcaa cggtagctta agttcagcgg cgtcaaacac
    32341 cacatgcgcc gtttcccact cccacgtttc cagtctgcgg taaggatggc aacgcatagc
    32401 ctgtactgca tcccagaccc ctaacccttt aagcaatgat accgaagccg cactaatcgc
    32461 cgagatccgc acgtccggct ggctgtcagc gacaaacggt gcaggttggg catgttcgat
    32521 aaccattacc gaaaatccgt gctgagccag tcccagcgcc agtgcgccgc cgaccattcc
    32581 tccgccgaca atggcaattt ccgttggttg atttgtcatg gtcaatcatc ctgttagcaa
    32641 tatgctttaa gtttacagga tttttacccc caagggctga taacgctcat actggtcaca
    32701 acggcagcaa agcattacac tatgcgccct gcattcctgg ctactatttc gcaagagcaa
    32761 gtcgatgacc aaaaaactcc atattaaaac ctggggctgt cagatgaacg agtacgattc
    32821 atcgaagatg gccgatctgc tggatgccac ccacggctat caactgaccg acgtggcgga
    32881 agaagcggat gtgctgctgc tgaacacctg ctcaatccgc gagaaggctc aggaaaaagt
    32941 cttccatcag ttgggtcgct ggaaactgtt aaaagagaag aatccagacc tgattatcgg
    33001 cgtcggcggc tgcgtggcat cgcaagaagg cgagcacatt cgccagcgcg cccactatgt
    33061 cgatattatt tttgggccgc aaacgctgca ccgtctgccg gagatgatca actccgtgcg
    33121 cggcgaccgc agcccggttg tagatatcag cttcccggaa atcgagaagt ttgaccgtct
    33181 gccggaaccg cgcgccgaag ggccgaccgc gtttgtctcc atcatggaag gctgcaataa
    33241 atattgcacc tactgcgtag tgccttacac ccgtggtgaa gaggtaagcc gtccgtccga
    33301 cgatattctg tttgagattg cccagcttgc tgctcagggc gtgcgtgaag ttaacctgct
    33361 cggccagaac gtgaacgcct ggcgcggtga gaactacgac ggcaccaccg gatcgtttgc
    33421 cgatctgctg cgtctggttg cggcgatcga cgggatcgat cgtattcgct ttaccaccag
    33481 ccatccgatc gaattcaccg acgatatcat cgaagtgtac cgcgacacgc cggagctggt
    33541 gagcttcctg catctgccgg tacagagcgg ttccgatcgc attctgaacc tgatgggacg
    33601 tacccataca gcgctggagt acaaagcgat catccgtaag ctgcgtgcgg cgcgtccgga
    33661 tattcagatc agttctgact tcatcgttgg cttccctggc gaaaccaccg aagacttcga
    33721 gaaaacgatg aagctgattg ccgacgtcaa tttcgacatg agctacagct ttatcttctc
    33781 cgctcgtcca ggtacaccag ccgccgatat ggttgatgat gttccggaag aagagaagaa
    33841 gcagcgcctg tatattctac aagaacgtat caatcagcaa gcaatggcat ggagccgccg
    33901 gatgctcggt accacccagc gtattctggt agaaggtaca tcgcgtaaga gcatcatgga
    33961 gctttccggc cgtactgaaa ataaccgcgt cgtcaacttc gaaggtacgc cggatatgat
    34021 cggtaaattc gtcgatgtag aaattaccga cgtctacccg aactctctgc gcggtaaagt
    34081 ggtgcgaact gaagatgaaa tgggtctgcg cgtggcagaa acaccggaat cggtgattgc
    34141 ccgtacccgc aaagaaaacg accttggcgt gggttattat cagccgtaat tctcaggccc
    34201 gccgttccgg tgggccttgt tttttctctc tttatcccca tctttatcgc aatgcttgcg
    34261 cctttttcct gtggcgtgaa taatttcctt ataggccggt gaatgttcag cttccggcgg
    34321 catacagata agaggaacgg tttgaacata gacactcgcg aaatcaccct ggagccagca
    34381 gacaatgcgc gtctgttgag cctgtgcggc ccgtttgatg acaacatcaa acaactcgaa
    34441 cgccgtctcg gcatcgaaat caatcgccgc gataaccact ttaaactgac cggccgtccg
    34501 atttgcgtca ccgctgcggc agacattctg cgtagcctgt atgtcgatac tgccccgatg
    34561 cgcggtcaga ttcaggatat cgaaccggaa cagatccacc ttgcgattaa agaagcgcgg
    34621 gtactggagc aaagcgcgga gagcgtgccg gagtacggca aagcggtcaa tatcaaaacc
    34681 aaacgcggcg taattaagcc gcgcacgcca aaccaggcgc agtacatcgc caatattctc
    34741 gaccatgaca ttaccttcgg cgttggcccg gcgggtacgg gtaaaaccta cctggcagtg
    34801 gctgcggcag ttgatgccct ggagcgtcag gaaattcgcc gtattctgct gactcgtccg
    34861 gcagtagaag ccggtgagaa actgggcttc ctgcctggcg atttaagcca gaaagtagac
    34921 ccgtatctgc gcccgctgta cgacgcgctg tttgaaatgc tgggctttga gaaagtcgag
    34981 aaactgattg agcgcaacgt tattgaagtc gcaccgctgg cctatatgcg tggtcgtacg
    35041 ctgaacgacg cgtttatcat tcttgatgag agccagaaca ccaccatcga acagatgaag
    35101 atgttcctga cccgtatcgg ttttaactca aaagcggtta tcaccggcga cgtcacacag
    35161 atcgacctgc cgcgtaatac caagtctggt ttgcgtcatg ccatcgaagt actggctgat
    35221 gtcgaagaga tcagctttaa cttcttccac agcgaagacg tggttcgtca cccggtggtg
    35281 gcgcgtatcg ttaacgccta tgaagcctgg gaagaagccg aacaaaaacg taaagcggcg
    35341 ctggcagcag aacgcaagcg cgaagaacag gaacaaaaat gagtcaggtg atcctcgatt
    35401 tacaactggc atgtgaagat aattccgggt taccggaaga gagccagttt cagacatggc
    35461 tgaatgcggt gatcccgcag tttcaggaag aatcggaagt gactattcgc gtggtcgata
    35521 ccgccgaaag ccacagtctg aatctgacct atcgcggtaa ggataagccg accaacgtgc
    35581 tctccttccc gtttgaagta ccgcctggca tggaaatgtc gctactgggc gatctggtta
    35641 tctgccgtca ggtggttgag aaggaagctc aggagcaagg caaaccactg gaggcgcact
    35701 gggcgcatat ggtggtgcac ggcagtctgc atttgttagg ttacgatcac atcgaagatg
    35761 acgaagcaga agaaatggaa gccatcgaaa cagagattat gcttgctctg ggctatgagg
    35821 atccgtacat tgccgagaaa gaataatttg ccaggcattt gactggcaga cgtccccgcc
    35881 gttggtgatt aacgacggcg ttttgattaa ctaacatgag aacccatacg acgccatgag
    35941 cgacgacaat tcacacagta gtgacacgat aagcaacaag aagggatttt tctccctgtt
    36001 actcagccaa cttttccacg gtgaaccgaa aaaccgtgac gaactgctgg cgctgatccg
    36061 tgattccggg cagaacgacc ttatcgacga agatacgcgc gatatgctcg aaggggtgat
    36121 ggacatcgca gaccaacgcg ttcgcgacat catgatcccc cgctcccaga tgattaccct
    36181 gaaacgcaac cagacgctgg acgaatgcct tgatgtcatc atcgagtccg cccactcacg
    36241 tttcccggtg attagcgaag acaaagatca cattgaaggg attctgatgg cgaaagactt
    36301 gctgccgttt atgcgcagcg atgctgaagc cttcagcatg gacaaagtgt tacgtcaggc
    36361 ggttgtcgtt cctgaaagta agcgcgtaga ccggatgctg aaagagtttc gctctcagcg
    36421 ttaccacatg gcgatcgtca ttgacgaatt cggtggggtt tccggtctgg tgaccattga
    36481 agacatcctg gaactgattg ttggtgagat tgaagacgag tatgacgaag aagatgatat
    36541 cgacttccgt cagctgagtc gtcatacctg gaccgtgcgc gcactggctt ctattgaaga
    36601 cttcaacgaa gcgttcggca cccactttag cgatgaagag gttgacacta tcggtggtct
    36661 ggtgatgcag gcatttgggc atcttccggc gcgtggcgaa accatcgaca tcgacggtta
    36721 ccagttcaaa gtggcgatgg ccgacagtcg gcgtattatt caggttcatg tcaaaatccc
    36781 ggatgactca ccccagccga agctggatga ataaaaccga aactggatag ataactacat
    36841 ggcttttgcc tcattaattg aacgccagcg cattcgcctg ctgctggcgt tattattcgg
    36901 tgcctgcgga acgctggcct tctctcctta cgacgtctgg cctgcggcga ttatttcgct
    36961 gatggggctt caggcgttga cctttaaccg ccgtccactc cagtctgccg ctattggctt
    37021 ttgctgggga tttggcctct ttggcagcgg tattaactgg gtctatgtca gcatcgcgac
    37081 ctttggcgga atgcctggcc cggttaacat cttcctggtg gtactgctgg cggcgtattt
    37141 gtcgctgtat accggactgt ttgctggcgt gctgtcgcgt ctgtggccga aaaccacctg
    37201 gctgcgcgtg gcgattgccg cccctgctct ctggcaagtg accgagtttc tgcgcggttg
    37261 ggtactaaca ggcttcccgt ggttacagtt cggctatagc cagattgatg gcccgttaaa
    37321 agggctggca ccgataatgg gcgtggaagc cattaacttc ctgctgatga tggttagcgg
    37381 cctgctggca ctggcgttgg tcaaacgcaa ctggcgtccg ctggtggtgg ccgtcgtgct
    37441 gtttgccctt cccttcccgc tgcgttacat ccagtggttt accccgcaac cggaaaaaac
    37501 tattcaggtt tcgatggttc agggcgatat tccgcaatcg ctgaaatggg acggagacca
    37561 gctacttaat acgctgaaga tatactacaa cgcaacggca ccgctgatgg gcaaatcatc
    37621 gttgattatc tggccggagt cggcgataac cgatctggaa attaatcagc aaccgttcct
    37681 caaagctctg gacggtgagt tgcgcgataa agggagctcg ctggtcaccg ggattgtcga
    37741 cgcgcgtctc aataaacaga accgctacga tacctacaac accatcatca cactgggtaa
    37801 aggcgcaccg tacagctatg aatcagccga tcgctataac aaaaaccatc tggtgccgtt
    37861 tggcgagttt gtcccactgg agtcgatact gcgtccgtta gcaccgttct ttgatttgcc
    37921 gatgtcttca ttcagccgcg gaccatatat tcagccaccg ctgtcgttga atggcattca
    37981 acttactgcg gccatttgct acgaaatcat tctcggcgag caagtgcgcg ataacttccg
    38041 cccggatacc gactatctgc tgactatctc caacgatgcg tggtttggta agtctattgg
    38101 tccatggcag cacttccaga tggcgcgaat gcgtgcgctg gagctggcgc gcccactgtt
    38161 gcgcagcacc aacaacggca ttacggcggt gattggcccg cagggtgaga ttcaggcgat
    38221 gatcccgcag ttcacccgcg aggtgttaac cactaacgtg acgccgacta ccggactcac
    38281 accgtacgca cgtaccggca actggccgct gtgggtgctg acggcactgt ttggttttgc
    38341 agctgtgctg atgagtctgc gagcacgtaa acattaatgt gcctgatgta tccggcatga
    38401 acaaaacata ctttctcatt gagttgtggg gcatgattat catgcccttt tttcttacgg
    38461 ttcagggata caagccacat cgccatattg gggatgccga ataattgcct gcggatcaag
    38521 cgagcgcgtc ctgtccattt ggattaacct gttcttcgcc ataacaaata tctttctacc
    38581 atccgctgaa aaacgaaggt gattaacgta gctggcagga taaacgccaa taatctgccc
    38641 ggcattaata ttgagcagga tgacataacc ttgcaggcca tatgacacca acgctaaata
    38701 ttgaccgtcc gggctaatac cctcaacgga ataaacgctc ctgctacacc attgcgtcat
    38761 aatgctggct tgttgatccc ctacgcttat acgtgaaatc ccctggctat cggcaaaaat
    38821 aatctcgcca ttttttggtc ggaatcccat cgcagaaacg ataccacgcg catcacctgt
    38881 gggaaagtct tctttctgat gcgtgatggt attgatgcgt acaatgtcgc ctctgaccat
    38941 accgggtatc aacaatgttt tttcatccgg ggaaagtgcc ataacccagg cgcctggttt
    39001 accgctcgtc cccgtcccgt cgtatttctc cagcgggata tccgtcacat gctgataagt
    39061 caccagatca atggctgaca cactattact ggtgcgattc atcacccagg cttttgtgcc
    39121 atcacgcgag aggataaggc tgtcaggatg ggcgccggtg gtaatcgact gaataatctc
    39181 ccccgtcgcc acgttaatga ccgatactgc attcgcttca aatgaagtcg cataaatcaa
    39241 cttttcatcc aggcttaatg ccagcgatct gacgccctga tctttacagc caccacaatg
    39301 tttgactgac ttttctttcg ctatgggcgc tgtaaaggta tgcaggattt tcttttgtcc
    39361 gaggtcgaaa acaacaatgc tgttggtgtt ttcaattgcg gcatacaacg tattaccatc
    39421 ccgactgatc gccatatcat ggatatcggc aatctcagcg agtaaatcac cactggcctg
    39481 ctcacgactt accggagcgg ataatgtttc cctgagggcg ttgcgctcct ccactaactg
    39541 ctgctttttt tgtcgtcgct cttgttcaat ctcctgcgga taaaacgctt cacgcaggct
    39601 ttgcctgacc tgtaaaactg attcatcaat ctgctgctgc acaaaccaga tagtcccggt
    39661 cagaagaaga gtgaccgcaa caataatccc gccttccgta acgagccgat tttgcccaac
    39721 taaacgcgca ctcagccaac ccaacgcgcc ccccgcaatc aggccgccaa tgtggcaagc
    39781 gttatctatt cctgcttgcc gggcaccata caacagtgtc agcgccacca tacccaacag
    39841 agaaaatact cgccgctggt ttttatggtg tgtacccgca cctgaagaaa gctgtgttgc
    39901 tatcgcagcg cccgctattc ccataattgc cccggatgcc ccaacgctga tgactatctg
    39961 ctcactttcg cgtaacgcat aacaggcgct gatcagccca ccaaagatgc cggagaataa
    40021 ccagataatc aggagtcgcc atttccccag aatacgttcc accgctcccc ccaccaccag
    40081 gagtgcaaac atattcatca gcaaatgaga aacactgctg tgcagaaaga cactgctcaa
    40141 taagcgccag gattcacccg acaacgtgag agaagcaacg ttagcaccga gatggataag
    40201 tgcggattga tcgacttctt cataactcat ccattgtgcg aggtaacaaa atatcccaac
    40261 acagagcgca aaaaggataa gggttattgc gggtaattga acatttaacg gcttaaccga
    40321 agatgcagac ataaggtgat tcttccttga aaagacaggt aaaaagcatt tttcccgtgt
    40381 gcagttatca tcccggcaaa cgtcccatgc cgaaaagata accctgtccg aaatatgact
    40441 atttcagcct aacattacaa tcgtaattaa ttcacctata agtatttttt ggcatgtaag
    40501 ttgctttatt tactgtgtaa gcaatgaaat cagccgcgac gcaaccgggt tgcaccacca
    40561 caaatcagcg gtagcaccaa aaaggtgcga tttaagaatt ttgcttcgga atggtgcggc
    40621 gcatctcgca aaaacaaaca ataaaacagc aatatcttta catttagcca aactaaatgt
    40681 tacctaacaa gcacaacact gcacaataaa gttgcagatg ataacaacac aaacactcac
    40741 aacgggtatc catgcgttct taacgcagaa gataaaggag ttggatatgc aattacgtaa
    40801 acctgccaca gcaatcctgg ccctggcgct ttccgcagga ctggcacagg cagatgacgc
    40861 cgccccggca gcaggcagta cgctggacaa aatcgccaaa aacggcgtga ttgtcgtcgg
    40921 tcaccgtgaa tcttcagtac ctttctctta ttacgacaat cagcaaaaag tggtgggcta
    40981 ctcgcaggat tactccaacg ccattgttga agcagtgaaa aagaaactca acaaaccaga
    41041 cttacaggtg aaattgattc cgatcacctc acaaaaccgt attccactgc tgcaaaacgg
    41101 cactttcgat tttgaatgcg gttctaccac caacaatgcc gaacgccaaa aacaggcggc
    41161 tttctctgac actattttcg tggtcggtac gcgcctgctg accaaaaagg gcggcgatat
    41221 caaagatttt gccgacctga aaggcaaagc cgtagtcgtc acttccggca ccacctctga
    41281 agttttgctc aacaaactga atgaagagca aaaaatgaat atgcgcatca tcagcgccaa
    41341 agatcacggt gactctttcc gtaccctgga aagcggtcgt gccgttgcct ttatgatgga
    41401 tgatgctctg ctggccggtg aacgtgcgaa agcgaagaaa ccagacaact gggaaatcgt
    41461 cggcaagccg cagtctcagg aagcctatgg ttgcatgttg cgtaaagacg atccgcagtt
    41521 caaaaagctg atggatgata ccatcgctca ggtgcagacc tccggtgaag cggaaaaatg
    41581 gtttgataag tggttcaaaa atccaattcc gccgaaaaac ctgaacatga atttcgaact
    41641 gtcagacgaa atgaaagcac tgttcaaaga accgaatgac aaggcactga actaattaca
    41701 agaaccaggg gcggaaattc cagccctctc gattgttacg tagcacggac agactatacg
    41761 cctggtggtc gttccccatc gggcctgaaa accgcaatac gctgggtaac aatcttcgag
    41821 ggtagcagtt aacgctgcta ccctcttttt tctggagtag atttatgtct atagactgga
    41881 actggggtat ttttttacaa caagccccgt tcggcaacac cacctatctt ggttggatct
    41941 ggagtggttt tcaggtgacg atcgctttgt cgatctgcgc ctggattatc gctttcctcg
    42001 tcggttcatt tttcggcatt ttacgtaccg ttccaaaccg ttttctctcc ggtctgggta
    42061 cgttgtatgt cgaactgttc cgcaacgtgc cgttgattgt gcaattcttt acctggtacc
    42121 tggtgatccc ggagctgctg ccagagaaaa tcggcatgtg gtttaaggca gagctggatc
    42181 ccaatattca gtttttcctt tcatccatgc tctgcctggg gctgtttacc gccgcccgtg
    42241 tttgcgaaca ggtacgcgcc gctattcagt cgctgccgcg tgggcaaaaa aatgccgcgc
    42301 tggcgatggg tctgacgcta ccgcaggctt accgctacgt actgctgcct aatgcttatc
    42361 gcgttatcgt cccgccgatg acctcagaga tgatgaacct ggtgaaaaac tccgccatcg
    42421 cctcgactat cggtctggtg gatatggcgg cgcaggcggg taaattgctc gattactcgg
    42481 cccacgcctg ggagtcgttt accgccatca cgctggcgta tgtattgatt aacgctttta
    42541 tcatgctggt gatgacgctg gttgaacgta aagttcgtct gcccggcaac atggggggca
    42601 aataatgtac gagtttgact ggagttccat tgttccttcc ctgccatatc tgctcgacgg
    42661 gctggtgatc acgctgaaaa tcaccgtcac ggcagtggtg attggtattt tgtggggcac
    42721 catgctggcg gtgatgcgct tgtccagctt tgcgcctgtt gcgtggtttg ccaaagccta
    42781 tgttaacgta ttccgctcga ttcctttagt catggttttg ctgtggtttt acctgattgt
    42841 gccgggattt ctgcaaaacg tgctgggatt atcgccaaaa aatgatatcc gtttaatttc
    42901 ggcaatggtg gcgttttcga tgtttgaagc ggcctactat tcagagatta tccgtgccgg
    42961 tattcaaagt atttcccgtg gtcagtcgag cgccgcgctg gcgttgggga tgactcactg
    43021 gcagtcgatg aaactgatta ttctgccgca ggctttccgc gcgatggtgc cgctgctgct
    43081 cactcagggc atcgtactgt tccaggatac ctcgctggtg tatgtgttat ctctggccga
    43141 tttcttccgt accgcttcaa ccattggtga gcgtgatggt acgcaggttg agatgatcct
    43201 gtttgccgga tttgtttatt tcgttattag ccttagcgcg tcgttgttgg tcagctactt
    43261 gaaaagaagg acagcataat gattaccctg aaaaatgttt caaaatggta tggtcacttt
    43321 caggtgctga ccgactgctc aaccgaagtg aaaaaaggcg aagtggtggt ggtttgcggc
    43381 ccgtctggtt ccggcaaatc aacgctgatt aaaaccgtca acggcctcga accggtgcag
    43441 caaggtgaaa tcaccgtcga tggcatcgtg gttaacgaca agaaaaccga tctggcaaag
    43501 ctgcgttccc gcgtcgggat ggtattccag catttcgagc tgttccctca tctgtcgatt
    43561 atcgaaaacc tgaccctggc gcaggtgaaa gtgcttaaac gcgataaagc gccggctcgt
    43621 gaaaaagcat tgaaattgct ggagcgcgtc gggctttccg cacatgccaa taagtttccg
    43681 gcgcagcttt ccggcggtca gcagcagcgt gtggcgatcg ctcgcgcatt gtgtatggat
    43741 cctattgcca tgctgttcga cgaaccgaca tcggcgctgg atccggagat gatcaacgaa
    43801 gtgctggacg tgatggtgga actggcgaac gaaggaatga ccatgatggt ggtgacccac
    43861 gaaatgggct ttgcccgtaa agtggcgaat cgggtgatct ttatggacga gggtaaaatt
    43921 gtcgaagact cgccgaaaga cgctttcttc gatgatccga aatcggaccg cgcaaaagac
    43981 ttcctcgcga aaatcctgca ttaatcactc tggcgcgcat acccaagtgc gcgccatctc
    44041 acgctttatc gtttcgctcc tcgtatctgt tacatcctag cggtaccctt gtcgcaaaga
    44101 agcacacaac aaggagcaac aatggcactg ccaattctgt tagattgcga cccaggtcat
    44161 gacgacgcta tcgcaatagt tcttgccctc gcctcaccag agcttgatgt caaagcaatt
    44221 acgtcttccg ccggaaacca gacaccagaa aaaaccttac gcaatgttct gcgtatgctg
    44281 accttgctta atcgcaccga tattccggta gcaggcggcg cggtaaaacc gttaatgcgt
    44341 aatttgatta ttgcggacaa tgtgcacggc gaaagcggtc tcgacggccc ggcattaccg
    44401 gaaccgacgt tcgcaccgca aaactgtacg gcggtggagc taatggcgaa aacgctgtgt
    44461 gaaagtgagg aacctatcac cattgtttca accggaccgc aaactaacgt tgccttgctg
    44521 ctcaatagcc acccggaact gcatagcaaa attgcccgta tcgtgattat gggcggcgca
    44581 atggggcttg gtaactggac gcctgcggct gaatttaata tttacgttga cccggaagcg
    44641 gcagaaattg tcttccagtc aggaattccg gtggtgatgg ccggtctgga tgttactcac
    44701 aaagcacaaa tccacgttga agacactgag cgtttccgcg cgattggcaa ccctgtttca
    44761 accattgttg ccgaactgct ggatttcttc ctcgaatatc ataaagacga aaaatggggc
    44821 tttgtcggcg caccactgca tgacccatgc accatcgcct ggctgttgaa accggagttg
    44881 tttaccactg ttgagcgctg ggttggcgtg gaaacacaag ggaaatatac ccagggtatg
    44941 acggttgttg attattatta tctgactggc aataaaccga atgccaccgt aatggtcgat
    45001 gttgatcgtc agggctttgt tgatttactg gctgatcgtc tgaaatttta cgcttaatca
    45061 ctcgccggat gacatcacgc catccggcac cactcatttc attacggctc gaacggacgt
    45121 ctctggaact gatcatgacc acatttcggg catagcgtca gcacttccgg tgtatagatc
    45181 gggagatgga agtgacattt ctcgcagacc agatttccca gccctaccac ttctccgctg
    45241 tgataaaccc cgtgatgatt gaggtcctgg aaaacttcgc gccattcaag ctgcgtttta
    45301 tcggtgatgt ctgccagctc ctgccacaag ctttctttaa tcacccgcat aaagacgctg
    45361 tcagattctt ctttcaggct ctcttcatag ctcatggcga actcttccag gtcacgtctg
    45421 acagctcgcg tcagctcatc gacctcggtt cgcgttaact cccctgtttt tattacgcgc
    45481 tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc cattgcgcag gcgttcgctc
    45541 agtgacgcga ccagttcacg gtaatattga gcaaccttgt tcatcgtttc atctcccggg
    45601 tcgttaactg actataataa tagacgttct ttgttccttg ctttgctaat acgcccacaa
    45661 actctgtaaa ttaatgtatg aataatccgg gcatcatttc aacgtcgagt gcgcgaaagg
    45721 ctgttttgac gcggacgttt gggctatgct atgcggatct gaaaaaccac ctcaacgcta
    45781 catttgtagc cgtattgaaa acaggaccac tggctgccat gcaagagcaa taccgcccgg
    45841 aagagataga atccaaagta cagcttcatt gggatgagaa gcgcacattt gaagtaaccg
    45901 aagacgagag caaagagaag tattactgcc tgtctatgct tccctatcct tctggtcgac
    45961 tacacatggg ccacgtacgt aactacacca tcggtgacgt gatcgcccgc taccagcgta
    46021 tgctgggcaa aaacgtcctg cagccgatcg gctgggacgc gtttggtctg cctgcggaag
    46081 gcgcggcggt gaaaaacaac accgcaccgg caccgtggac gtacgacaac atcgcgtaca
    46141 tgaaaaacca gctcaaaatg ctgggctttg gttatgactg gagccgcgag ctggcaacct
    46201 gtacgccgga atactaccgt tgggaacaga aattcttcac cgagctgtat aaaaaaggcc
    46261 tggtatataa gaagacttct gcagtcaact ggtgcccgaa cgaccagacc gtactggcga
    46321 acgaacaagt tatcgacggc tgctgctggc gctgcgatac caaagttgag cgtaaagaga
    46381 tcccgcaatg gtttatcaaa atcaccgctt acgctgacga gctgctcaac gatctggata
    46441 aactggatca ctggccagac accgttaaaa ccatgcagcg taactggatc ggtcgttccg
    46501 aaggcgtgga aatcaccttc aacgttaacg actatgacaa cacgctgacc gtttacacta
    46561 cccgcccgga cacctttatg ggttgtacct acctggcggt ggctgcgggt catccgctgg
    46621 cacagaaagc ggcggaaaat aatcctgaac tggccgcctt tattgacgaa tgccgtaaca
    46681 ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa aggcgtcgat actggcttta
    46741 aagcggttca cccattaacg ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga
    46801 tggaatacgg cacgggcgca gttatggcgg ttccgggtca cgaccagcgc gactacgagt
    46861 ttgcctctaa atacggcctg aacatcaaac cggttatcct ggcagctgac ggctctgagc
    46921 cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct gttcaactct ggcgagttca
    46981 acggtcttga ccatgaagcg gccttcaacg ccatcgccga taaactgact gcgatgggcg
    47041 ttggcgagcg taaagtgaac taccgcctgc gcgactgggg tgtttctcgt cagcgttact
    47101 ggggcgcgcc gattccgatg gtgacgctgg aagacggtac cgtaatgccg accccggacg
    47161 accagctgcc ggtgatcctg ccggaagatg tggtgatgga cggcattacc agcccgatta
    47221 aagcagatcc ggagtgggca aaaactaccg ttaacggtat gccagcgctg cgtgaaaccg
    47281 acactttcga cacctttatg gagtcctcct ggtactatgc gcgctacact tgcccggagt
    47341 acaaagaagg tatgctggat tccaaagcgg ctaactactg gctgccggtg gatatctaca
    47401 ttggtggtat cgaacacgcc attatgcacc tgctctactt ccgcttcttc cacaaactga
    47461 tgcgtgatgc aggcatggtg aactctgacg aaccagcaaa acagttgctg tgtcagggta
    47521 tggtgctggc agatgccttc tactatgttg gcgaaaatgg cgaacgtaac tgggtttccc
    47581 cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg
    47641 caggccatga actggtttat accggcatga gcaaaatgtc caagtcgaag aacaacggta
    47701 tcgacccgca ggtgatggtt gaacgttacg gcgcggacac cgttcgtctg tttatgatgt
    47761 ttgcttctcc ggctgatatg actctcgaat ggcaggaatc cggcgtggaa ggggctaacc
    47821 gcttcctgaa acgtgtctgg aaactggttt acgagcacac agcgaaaggt gatgttgcgg
    47881 cactgaacgt tgatgcgctg actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa
    47941 cgatcgctaa agtgaccgat gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg
    48001 cgattatgga gctgatgaac aaactggcga aagcaccgac cgatggcgag caggaccgcg
    48061 ccctgatgca ggaagcactg ctggccgttg tccgtatgct taacccgttc accccgcaca
    48121 tctgcttcac gctgtggcag gaactgaaag gcgaaggcga tatcgacaac gcgccgtggc
    48181 cggttgctga cgaaaaagcg atggtggaag actccacgct ggtcgtggtg caggttaacg
    48241 gtaaagtccg tgcaaaaatc accgttccgg tggacgcaac ggaagaacag gttcgcgaac
    48301 gtgctggcca ggaacatctg gtagcaaaat atcttgatgg cgttaccgtg cgtaaagtga
    48361 tttacgtacc cggtaaactc ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg
    48421 atatctggca acattgttgt tatctctggc ggtgttaatc accgccgggt gtggctggca
    48481 tctgcgtgat accacgcagg ttccttccac tatgaaggtc atgatcctgg actcaggcga
    48541 tccgaacggg ccattaagcc gtgcggtgcg taaccagtta cgtctaaatg gtgtcgagtt
    48601 gcttgataaa gaaaccacgc gtaaggacgt tccatccctg cgtttgggta aagtgagcat
    48661 cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca gcagagtatc agatgatcat
    48721 gacggtcaat gcgaccgtgt tgatccccgg ccgtgacatc tacccgatta gcgccaaagt
    48781 cttccgttcg ttcttcgata acccgcaaat ggcgttagcg aaagataacg aacaagacat
    48841 gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg attcgtaagc tgccaagcat
    48901 ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg accacaacgg atactccggc
    48961 aacgcctgca cgcgtctcta ccacgctggg taactgatga ttcggttgta cccggaacaa
    49021 ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc ttttacttgg taacgatcct
    49081 ctgttattgc aggaaagcca ggacgctatt cgtcaggtag ctgcggtaca aggattcgaa
    49141 gaacaccaca ctttttccat tgatcccaac actgactgga atgcgatctt ttcgttatgc
    49201 caggccatga gtctgtttgc cagtcgacaa acgctattgc tgttgttacc agaaaacgga
    49261 ccgaatgcgg cgatcaatga acaacttctc acactcaccg gacttctgca tgacgacctg
    49321 ctgttgatcg tccgcggcaa taaattaagc aaagcgcaag aaaatgccgc ctggtttact
    49381 gctcttgcga atcgcagcgt gcaggtgacc tgtcagacac cggagcaggc tcagcttccc
    49441 cgctgggttg ctgtgcgcgc aaaacagctc aacttagagc tggatgacgc ggcgaatcag
    49501 gtgctctgct actgttatga aggtaacctg ctggcgctgg ctcaggcact ggagcgttta
    49561 tcgctactct ggccagacgg caaactgaca ttacctcgcg ttgaacaggc ggtgaatgat
    49621 gccgcgcatt tcaccccttt tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc
    49681 gcgttgcata ttcttcagca actgcgtctg gaaggcagcg aaccggttat tttgttgcgc
    49741 acattacaac gtgaactgtt gttactggtt aacctgaaac gccagtctgc ccatatgcca
    49801 ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc gccggggcat gatgggcgag
    49861 gcgttaaatc gcttaagcca tccgcagtta cgtcaggccg tgcaactcct gacacgaacg
    49921 gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg cagagcttga agggttatct
    49981 cttctgttgt gccataaacc cctggcggac gtatttatcg acggttgata tgaaatcttt
    50041 acaggctctg tttggcggca cctttgatcc ggtgcactat ggtcatctta aacccgtgga
    50101 aacgctggcg aatttgattg gtctgacgcg ggtcacaatc atccctaata atgttcctcc
    50161 gcatcgtccc cagccggaag caaacagcat gcagcgtaaa cacatgcttg aactggcgat
    50221 tgccgacaag ccattattta ctcttgatga acgcgagcta aagcgcaatg ccccctctta
    50281 cactgcgcaa acactgaaag agtggcggca ggagcaagga ccggacgtgc cgctggcgtt
    50341 tattattggt caggattcat tgctgacctt tccgacctgg tacgaatacg aaacgatact
    50401 cgacaatgca catttgatcg tctgtcggcg tcctggttac ccacttgaaa tggcacaacc
    50461 acaatatcag caatggctgg aggatcattt gacacataac ccggaagatc ttcaccttca
    50521 gcctgccggt aaaatttatc tggctgaaac gccgtggttt aacatctcgg cgaccatcat
    50581 ccgcgaacgt ttgcaaaatg gtgaatcgtg tgaggattta ttaccggaac cggtactgac
    50641 ttacattaac caacaaggct tgtatcgctg atacctgtcg ttacggaggg catgatgcga
    50701 ctgtggttaa ttcgtcatgg tgaaacgcaa gcgaatgtcg atggtctgta cagcggtcat
    50761 gcgcccacac ccctgaccgc gcgcggtatt gagcaagcgc aaaatctgca tacgctgcta
    50821 catgatgttt cctttgacct ggttttatgc agtgaactgg aacgggcaca gcataccgcg
    50881 cgactggttc ttagtgaccg ccagcacccc gttcatatca tccctgaact caatgaaatg
    50941 ttttttggcg actgggagat gcgacatcat cgtgacctca tgcaagaaga tgccgaaaac
    51001 tatagcgcgt ggtgcaatga ctggcagcat gctatcccca cgaacggtga aggatttcag
    51061 gcattttcgc aacgtgtgga acgatttatc gcaaggctta gtgaatatca gcactatcag
    51121 aatattttag tcgtcagcca tcagggcgta ctgagtctgt taattgctcg tttaattggc
    51181 atgcctgccg agtctatgtg gcatttccgc gttgaccagg ggtgttggag cgccattgat
    51241 atcaaccaaa aattcgcgac gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaat
    51301 gcatgacttt tctgtttttt tacgggtaag ccgcaacgac cattgacagc cccgggcagg
    51361 ctgatattct ccgcagccag actttttccg ccagacacga ctttgtagaa attgttttac
    51421 aaaaatggcg atgcaatctg cggcgcgggg tgggatgata gcccactttc gaaagccgat
    51481 tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat actgacagac catttttatc
    51541 tatttgattc acccaggggg aaaacttgca gggtaaagca ctccaggatt ttgttatcga
    51601 caaaattgat gacctcaaag gtcaggacat catcgcctta gacgttcagg gcaaatccag
    51661 catcaccgac tgcatgatca tctgtacggg tacgtccagc cgccatgtta tgtccattgc
    51721 tgatcacgtt gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggcg tagaaggtga
    51781 aaacagcgcc gactggattg tcgtggattt gggcgatgtg attgtccatg tcatgcagga
    51841 agagagccgt cgcctgtatg aactggaaaa actctggagt taatgcgtga agctgcaact
    51901 tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg agtacctgcg
    51961 tcgttttccg aaagatatgc ccttcgagct gattgaaatt ccggccggaa aacgcggcaa
    52021 gaatgcggac atcaagcgca tactcgacaa agagggtgag cagatgttgg cggccgcagg
    52081 caaaaaccgc attgtcaccc tcgatattcc aggcaagccc tgggatacgc cgcagttagc
    52141 cgctgagctg gaacgctgga agctggatgg tcgcgacgtc agtctactga ttggcgggcc
    52201 tgaagggttg tcgcctgcct gtaaagcggc ggcggagcag agctggtcgc tgtcggcact
    52261 tacccttccc catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag
    52321 catcaccacc aaccatcctt atcaccgtga gtgataagtg agctttgagt agaaaacgca
    52381 gcggatgaaa ctacagaact cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg
    52441 ccgggcgctg gtcgcctttt tggggatttt gctgctgacc ggcgtgctta tcgccaacct
    52501 gtataatctg caaattgttc gctttaccga ctaccagacc cgctctaatg aaaaccgcat
    52561 taagctggtg cctatcgcac ccagccgcgg cattatctac gaccgtaacg gtatccctct
    52621 ggctctcaac cgcactatct accagataga aatgatgccg gagaaagtcg ataacgtgca
    52681 gcaaacgctg gacgctttgc gcagcgtggt tgatctgacc gatgatgata ttgcggcatt
    52741 ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt ccggtgaaaa ctaacctgac
    52801 cgaagtacaa gtagctcgct ttgccgtcaa ccagtaccgt tttccgggtg ttgaagttaa
    52861 aggctataaa cgtcgttact atccttacgg ttcggcgttg acccacgtca tcggctatgt
    52921 gtcgaaaatc aacgataaag acgtcgaacg cctgaataat gacggcaaac tggccaacta
    52981 tgcggcaacg catgatatcg gtaagctggg cattgaacgt tactatgaag acgtgctgca
    53041 cggtcagacc ggttatgaag aggttgaagt taacaaccgt gggcgtgtta tccgccagtt
    53101 aaaagaagta ccaccgcaag ccggacacga tatttacctg acgctggatc tcaaactcca
    53161 gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct gtggtagtca ccgatccgcg
    53221 tacaggtggg gtgttggcgc tggtttccac gcctagttat gacccaaacc tgtttgttga
    53281 cggtatctcc agcaaagatt attccgcctt gctgaacgac ccgaatacac cgctggtgaa
    53341 ccgcgccaca cagggggttt atcctcccgc gtctacagtt aaaccctatg ttgcggtttc
    53401 ggcattgagt gccggggtga tcacgcgcaa tacgacgctg tttgaccccg gctggtggca
    53461 actgccaggg tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa
    53521 tgtcacaaga tcgctggaag aatctgcgga taccttcttc tatcaggtgg cctacgatat
    53581 ggggatcgat cgcctctccg aatggatggg taaattcggt tatggtcatt acaccggtat
    53641 cgacctggcg gaagaacgtt ccggcaacat gcctacccgc gaatggaaac agaaacgctt
    53701 taaaaaaccg tggtatcagg gtgacaccat tccggttggt atcggtcagg gttactggac
    53761 agcgacccca atccagatga gtaaggcact gatgatcctg attaatgacg gtatcgtgaa
    53821 ggttccgcat ttgctgatga gcaccgccga agacggcaaa caggtgccat gggtacaacc
    53881 gcatgaaccg cccgtcggcg atattcattc cggttactgg gagctggcga aagacggtat
    53941 gtacggtgtt gctaaccgcc ctaacggtac ggcgcataaa tattttgcca gcgcaccgta
    54001 caaaattgcg gcgaaatccg gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta
    54061 taatgcgcac aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc
    54121 atacaacaat ccgcaagtgg ctgtcgccat gattctggag aacggtggcg cgggtccggc
    54181 ggttggtacg ctgatgcgcc agatcctcga ccacattatg ctgggtgata acaacaccga
    54241 tctgcctgcg gaaaatccag cggttgccgc agcggaggac cattaatcat gacagataat
    54301 ccgaataaaa aaacattctg ggataaagtc catctcgatc ccacaatgct gctgatctta
    54361 ctggcattgc tggtttacag cgccctggtt atctggagcg ccagcggtca ggatattggc
    54421 atgatggagc gtaaaatcgg ccagattgcg atgggtctgg tcatcatggt ggtgatggcg
    54481 caaattcctc cacgcgttta tgaaggctgg gccccctatc tctatatcat ctgtattatt
    54541 ttgctggtgg cggtagatgc tttcggtgcc atctctaaag gcgctcaacg ctggctggac
    54601 ctcggtattg ttcgttttca accgtcggaa attgccaaga tagccgtacc gttgatggtt
    54661 gcacgcttta tcaaccgcga cgtttgccca ccttcattga agaatacagc tatcgcgctg
    54721 gtgctgatat ttatgcccac gctgctggtg gctgcacagc ctgacttggg aacatcaatc
    54781 ctcgttgcgc tttccggcct gtttgtactg ttcctctctg gccttagctg gcgtctgatt
    54841 ggcgtcgcag tagtgctggt agcggcgttc attccgattc tgtggttctt tctgatgcat
    54901 gattaccagc gccagcgcgt aatgatgctc ctggacccgg aatcagaccc actcggcgca
    54961 ggctatcaca ttattcagtc taaaattgct attggctccg gcggattacg cggcaaaggc
    55021 tggctgcacg gcactcagtc acagcttgaa tttctccccg aacgccatac cgactttatc
    55081 ttcgcggtac tggcggaaga gctgggatta gtgggcattt tgattctgct cgctctctac
    55141 attctgctga tcatgcgcgg gctgtggata gccgccagag cgcaaaccac ctttggtcgc
    55201 gtcatggctg gcggcttaat gctgatatta ttcgtttatg tcttcgtaaa tattggtatg
    55261 gtaagcggta ttctgccggt tgtaggggtt ccgctcccac tggtcagtta tggaggatcg
    55321 gcgctaattg tgctgatggc tgggttcggg attgtaatgt caatccacac ccacaggaaa
    55381 atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat
    55441 cgcggcagga atgctcgcgg catgtacaag cgatgatggt cagcaacaga cggtaagtgt
    55501 accgcagcct gcggtatgta acggccccat agttgaaatt agcggggcag acccgcgttt
    55561 cgaaccactg aacgcgacgg caaatcagga ttaccagcgc gacgggaaaa gctacaaaat
    55621 cgtgcaggat ccgtctcgat ttagccaggc gggactggcg gcaatctatg atgccgaacc
    55681 cggcagtaac ctgacggcct ctggcgaagc tttcgatccg acacagttga cggcggccca
    55741 tccaacgctt ccaatcccca gctacgccag aatcactaac ctggctaacg ggcgaatgat
    55801 cgtggtgcgc attaatgatc gcggccttta tggcaacgac cgcgtgattt cgctttctcg
    55861 cgcggcagct gaccgtctta acacgtcaaa caacaccaaa gttcgtatcg atccgattat
    55921 tgtcgcccag gatggttcgc tttctggtcc tggtatggct tgtaccacag tcgccaaaca
    55981 gacttacgcc ctgccagcac ctcccgattt aagcggtggc gcgggaacaa gttcagtgcc
    56041 tgtcccgcag ggtgacattc ttccggtcag taattcgacg ctaaaaagcg aagatccgac
    56101 cggcgcgccg gtaaccagca gcggtttcct cggcgcacca acgaccttag cgcctggtgt
    56161 actggaaggc agcgaaccga cgcctgcgcc acagcccgtt gttacagctc cgtcgacaac
    56221 gcctgcaacc tcacctgcaa tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa
    56281 ctttatggtg caagtcgggg ccgttagcga tcaggctcgt gcgcaacagt accaacagca
    56341 actgggacag aagttcggcg tccccggtcg cgtaactcaa aatggcgcgg tctggcggat
    56401 ccagcttggc ccattcgcca gcaaagccga agccagtacc ttgcagcaac gtttgcaaac
    56461 cgaagcccaa ttacagtcat ttattaccac cgcgcagtag cgtaaagcag gcatctgatg
    56521 tgtcaatttc tgtaaatgac gttaacaaag tcacgcggaa agtcagatgc ctgccggtag
    56581 tgcatttgct atagtagggc acttttttta attccatcac ggatgtcgta gttcagacca
    56641 tgaataccat tttttccgct cgtatcatga agcgcctggc gctcaccacg gctctttgca
    56701 cagcctttat ctctgctgca catgccgatg acctgaatat caaaaccatg atcccgggtg
    56761 taccgcagat cgacgcggag tcctacatcc tgattgacta taactccggc aaagtactcg
    56821 ccgaacagaa cgctgatgtc cgccgcgatc ctgccagcct gaccaaaatg atgaccagtt
    56881 acgttatcgg ccaggcaatg aaagccggta aatttaaaga aactgattta gtcactatcg
    56941 gcaacgacgc atgggccacc ggtaacccgg tgtttaaagg ttcttcgctg atgttcctca
    57001 aaccgggcat gcaggttccg gtttctcagc tgatccgcgg tattaacctg caatcgggta
    57061 acgatgcttg tgtcgccatg gccgattttg ccgctggtag ccaggacgct tttgttggct
    57121 tgatgaacag ctacgttaac gccctgggcc tgaaaaatac ccacttccag acggtacacg
    57181 gtctggatgc tgatggtcag tacagctccg cgcgagatat ggcgctgatc ggccaggcgt
    57241 tgatccgtga cgtaccgaat gaatactcga tctataaaga aaaagaattt acgtttaacg
    57301 gtattcgcca gctgaaccgt aacggcctgt tatgggataa cagcctgaat gtcgacggca
    57361 tcaaaaccgg acacactgac aaagcaggtt acaaccttgt tgcttctgcg actgaaggcc
    57421 agatgcgctt gatctctgcg gtgatgggcg gacgtacttt taaaggccgt gaagccgaaa
    57481 gtaaaaaatt gctaacctgg ggcttccgtt tcttcgaaac cgttaatcct ctgaaagtag
    57541 gtaaagagtt cgcctctgaa ccggtttggt ttggtgattc tgatcgcgct tcgttagggg
    57601 ttgataaaga cgtgtacctg accattccgc gtggtcgcat gaaagatctg aaagccagct
    57661 atgtgctgaa cagcagtgaa ttgcatgcgc cgctgcaaaa gaatcaggtc gtcggaacta
    57721 tcaacttcca gcttgatggc aaaacgatcg aacaacgccc gctggttgtg ctgcaagaaa
    57781 tcccggaagg taacttcttc ggcaaaatca ttgattacat taaattaatg ttccatcact
    57841 ggtttggtta aaaattgaac acttgaaagt gtaatttccg tccccatata ctaagcatca
    57901 gtaaaaaact cccgccttct ggcgggagtt gctatttaat tacgttacgc cggagctgac
    57961 atgaaaacca aacttaacga actgcttgaa ttccctactc cttttactta caaagttatg
    58021 gggcaggcgt tacctgagct ggttgatcag gtggttgaag tggtacagcg ccatgcgcca
    58081 ggtgactaca ccccaacggt aaaaccaagc agcaaaggca actaccactc ggtatctatc
    58141 actatcaacg ccactcatat cgagcaggtt gaaacactgt atgaagaact gggcaaaatc
    58201 gatattgtcc gcatggttct gtaactcgct tctccgttac ccggctcctg gtcgggtaac
    58261 tcccctctcc tcgctgtgat atacttttcc cccactttta ctcattctcc acggagatgc
    58321 cgttttgtat caggataaaa ttcttgtccg ccagctcggt cttcagcctt acgagccaat
    58381 ctcccaggct atgcatgaat tcaccgatac ccgcgatgat agtacccttg atgaaatctg
    58441 gctggtcgag cactatccgg tattcaccca agggcaggca ggaaaggcgg agcacatttt
    58501 aatgccgggt gatattccgg tgatccagag cgatcgcggt gggcaggtga cttatcacgg
    58561 gccggggcaa caggtgatgt atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg
    58621 tgaactggtg accttgcttg agcaaacagt ggtgaatacc ctggctgaac tgggtataga
    58681 agcgcatcct cgggctgacg cgcctggtgt ctatgtcggg gaaaagaaaa tttgctcact
    58741 gggtttacgt attcgacgcg gttgttcatt ccacggtctg gcattaaacg tcaatatgga
    58801 tctttcacca tttttacgta ttaatccttg tgggtatgca ggaatggaaa tggctaaaat
    58861 atcacaatgg aaacccgaag cgacgactaa taatattgca ccacgtttac tggaaaatat
    58921 tttagcgcta ctaaacaatc ctgacttcga atatattacc gcttaattcc atacatcaat
    58981 ggcccaattt acactgggtc attactcata atacctgcta atgctttaca tctccttcgc
    59041 gtttgtatat tttctttatc acttgcagaa atgcgatgct acgccttgct ccgtagccca
    59101 cctaattgat taacccattg aaataattga ccatggtgag
//
LOCUS       DAZGHF010000015        53792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGHF010000015 DAZGHF010000000
VERSION     DAZGHF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.015"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            164..1747
                     /gene="yegH"
                     /locus_tag="ACHK3A_001017"
     CDS             164..1747
                     /gene="yegH"
                     /locus_tag="ACHK3A_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO8739134.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            1822..2160
                     /locus_tag="ACHK3A_001018"
     CDS             1822..2160
                     /locus_tag="ACHK3A_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8739135.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSNIDLAKRRYK
                     ELVQERKNEN"
     gene            2150..2440
                     /locus_tag="ACHK3A_001019"
     CDS             2150..2440
                     /locus_tag="ACHK3A_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8739136.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2493..4346)
                     /gene="asmA"
                     /locus_tag="ACHK3A_001020"
     CDS             complement(2493..4346)
                     /gene="asmA"
                     /locus_tag="ACHK3A_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO8739137.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQVNWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLKETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVIGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4368..4949)
                     /gene="dcd"
                     /locus_tag="ACHK3A_001021"
     CDS             complement(4368..4949)
                     /gene="dcd"
                     /locus_tag="ACHK3A_001021"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO8739138.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5041..5682)
                     /gene="udk"
                     /locus_tag="ACHK3A_001022"
     CDS             complement(5041..5682)
                     /gene="udk"
                     /locus_tag="ACHK3A_001022"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO8739139.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            6000..9317
                     /gene="dgcE"
                     /locus_tag="ACHK3A_001023"
     CDS             6000..9317
                     /gene="dgcE"
                     /locus_tag="ACHK3A_001023"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO8739140.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVILLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9426..10274)
                     /gene="alkA"
                     /locus_tag="ACHK3A_001024"
     CDS             complement(9426..10274)
                     /gene="alkA"
                     /locus_tag="ACHK3A_001024"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO8739141.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEC
                     RGVVTAIPDIASHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPLRSYALLHIWYTEG
                     WQPDEA"
     gene            10408..11760
                     /gene="yegD"
                     /locus_tag="ACHK3A_001025"
     CDS             10408..11760
                     /gene="yegD"
                     /locus_tag="ACHK3A_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8739142.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11773..13712)
                     /gene="yegI"
                     /locus_tag="ACHK3A_001026"
                     /pseudo
     CDS             complement(11773..13712)
                     /gene="yegI"
                     /locus_tag="ACHK3A_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(13709..14470)
                     /gene="pphC"
                     /locus_tag="ACHK3A_001027"
     CDS             complement(13709..14470)
                     /gene="pphC"
                     /locus_tag="ACHK3A_001027"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO8739143.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYTNMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSGPAVNERTDDDKTLALALWLP"
     gene            complement(14467..15126)
                     /gene="yegL"
                     /locus_tag="ACHK3A_001028"
     CDS             complement(14467..15126)
                     /gene="yegL"
                     /locus_tag="ACHK3A_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO8739144.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15347..15406)
                     /gene="ibsA"
                     /locus_tag="ACHK3A_001029"
     CDS             complement(15347..15406)
                     /gene="ibsA"
                     /locus_tag="ACHK3A_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO8739145.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15679..15735)
                     /locus_tag="ACHK3A_001030"
     CDS             complement(15679..15735)
                     /locus_tag="ACHK3A_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8739146.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            complement(16007..16063)
                     /locus_tag="ACHK3A_001031"
     CDS             complement(16007..16063)
                     /locus_tag="ACHK3A_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8739147.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(16335..16391)
                     /locus_tag="ACHK3A_001032"
     CDS             complement(16335..16391)
                     /locus_tag="ACHK3A_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8739148.1"
                     /translation="MMKILIIVVLLVVSYPAY"
     gene            16670..17917
                     /gene="mdtA"
                     /locus_tag="ACHK3A_001033"
     CDS             16670..17917
                     /gene="mdtA"
                     /locus_tag="ACHK3A_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO8739149.1"
                     /translation="MKSSYKSRWVMVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17917..21039
                     /gene="mdtB"
                     /locus_tag="ACHK3A_001034"
     CDS             17917..21039
                     /gene="mdtB"
                     /locus_tag="ACHK3A_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO8739150.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGQGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            21040..24117
                     /gene="mdtC"
                     /locus_tag="ACHK3A_001035"
     CDS             21040..24117
                     /gene="mdtC"
                     /locus_tag="ACHK3A_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO8739151.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRIGLNPQALFNQGVSLDDVRSAISNANVRKPQG
                     ALEDDTHRWQIQTNDELKTAAEYQPLIIHYNNGGALRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            24118..25533
                     /gene="mdtD"
                     /locus_tag="ACHK3A_001036"
     CDS             24118..25533
                     /gene="mdtD"
                     /locus_tag="ACHK3A_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8739152.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQIGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAITTLMLMPNYTMQTRCFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNCALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSAQ"
     gene            25530..26933
                     /gene="baeS"
                     /locus_tag="ACHK3A_001037"
     CDS             25530..26933
                     /gene="baeS"
                     /locus_tag="ACHK3A_001037"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO8739153.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26930..27652
                     /gene="baeR"
                     /locus_tag="ACHK3A_001038"
     CDS             26930..27652
                     /gene="baeR"
                     /locus_tag="ACHK3A_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO8739154.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLMIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLEALDEE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27843..28175
                     /gene="yegP"
                     /locus_tag="ACHK3A_001039"
     CDS             27843..28175
                     /gene="yegP"
                     /locus_tag="ACHK3A_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO8739155.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            28323..29684
                     /gene="yegQ"
                     /locus_tag="ACHK3A_001040"
     CDS             28323..29684
                     /gene="yegQ"
                     /locus_tag="ACHK3A_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO8739156.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTTFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29957..30175)
                     /gene="ogrK"
                     /locus_tag="ACHK3A_001041"
     CDS             complement(29957..30175)
                     /gene="ogrK"
                     /locus_tag="ACHK3A_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFO8739157.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(30257..31420)
                     /locus_tag="ACHK3A_001042"
     CDS             complement(30257..31420)
                     /locus_tag="ACHK3A_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO8739158.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDISGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(31420..31899)
                     /locus_tag="ACHK3A_001043"
     CDS             complement(31420..31899)
                     /locus_tag="ACHK3A_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8739159.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATRFTGMTGAG"
     gene            complement(31914..34361)
                     /locus_tag="ACHK3A_001044"
     CDS             complement(31914..34361)
                     /locus_tag="ACHK3A_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8739160.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAETMAGVKLLMSGYEFAQK
                     NSELQAVLGVAKDSAEMTALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTVASTGKLDKLTAAFKASDGKTSELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALVLTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFTNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(34354..34509)
                     /locus_tag="ACHK3A_001045"
     CDS             complement(34354..34509)
                     /locus_tag="ACHK3A_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO8739161.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGSTNE"
     gene            complement(34506..34781)
                     /locus_tag="ACHK3A_001046"
     CDS             complement(34506..34781)
                     /locus_tag="ACHK3A_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO8739162.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(34838..35356)
                     /locus_tag="ACHK3A_001047"
     CDS             complement(34838..35356)
                     /locus_tag="ACHK3A_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO8739163.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATSADAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEVDTGEGKQGEDTESKLPVICTYFKLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(35369..36559)
                     /locus_tag="ACHK3A_001048"
     CDS             complement(35369..36559)
                     /locus_tag="ACHK3A_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFO8739164.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALMGAESVTGVKPRILGVPGLDTKEVAVALASVCQELNAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQKPGTDADLLNEAGVTTLVRRDGFRFWGN
                     RTCSDDPLFLFESYTRTAQVLADTMAEAHMWAIDKPITATLIRDIIDGINAKFRELKN
                     NGYIVDGTCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITSRYLASLVTS
                     VNSN"
     gene            36972..37415
                     /locus_tag="ACHK3A_001049"
     CDS             36972..37415
                     /locus_tag="ACHK3A_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO8739165.1"
                     /translation="MLDIRMSRPSEASEIIQIWKSSVDATHDFLTGHDRQEIEKEVIG
                     FFSETPVWVATNQDDQPLGFMFLHEGHLEALFVAGSARGLGVGKRLISHALALHPELS
                     VDVNEQNQQAVGFYQHMGFQVSGRSDLDNQGRPYPLLHLRMANNM"
     gene            complement(37436..38020)
                     /locus_tag="ACHK3A_001050"
     CDS             complement(37436..38020)
                     /locus_tag="ACHK3A_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO8739166.1"
                     /translation="MDKKFKNQIVDSDIARLLLKHRKARNLTVTELAQRSGVSQAMIS
                     KIERGISSPSATILSRLANAMNVTLSKLFAELEQHQNSLVRFAHQQQHWTDEQSGITR
                     WSLSPAGACPELIKVEIPAMGELTIPASANEHLSGQTLWMMSGSLDFRVKNQTHHLRA
                     GDCLALTFASEYQMSNPDSEQSCSYIVAFSQKNQ"
     gene            complement(38256..38783)
                     /locus_tag="ACHK3A_001051"
     CDS             complement(38256..38783)
                     /locus_tag="ACHK3A_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO8739167.1"
                     /translation="MQHLKNITAGNPKTVAQYQLTKNFDVIWLWSEEGKNWYEEVSNF
                     QEDTIKIVYDENNIIVGITRDASTLNPEGFSVVEVPDITSNRRADDSGKWMFKDGAVI
                     KRIYTADEQEQQAESQKAALLSEAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(38787..40832)
                     /locus_tag="ACHK3A_001052"
     CDS             complement(38787..40832)
                     /locus_tag="ACHK3A_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8739168.1"
                     /translation="MSTKFKTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSMSETLAATPKAVKTVMDETNKKAPLNSPALTGTPTTPTARQGTNNTQ
                     IANTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTA
                     LAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQETVNRAGNAV
                     QKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSYMWFETGDNGNEYFKW
                     RSRQSTTTKDLMNLKWDALYVLVKALFSSEVKISTVNALRIFNSSFGAIFRRSEENLY
                     IIPTRENEGENGDIGSLRPFGINLRTGVVSVGNGARIDGGLALGTNNALGGNSIVLGD
                     NDTGFKQNGDGNLDVYANNVHVMRFVSGSIQSNKTINITGRVNPSDYGNFDSRYVRDI
                     RLGTRVVQTMQKGVMYEKAGHVITGLGIVGEVDGDDPAVFRPIQKYINGTWYNVAQV"
     gene            complement(40843..41373)
                     /locus_tag="ACHK3A_001053"
     CDS             complement(40843..41373)
                     /locus_tag="ACHK3A_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO8739169.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDSCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWETSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(41366..42274)
                     /locus_tag="ACHK3A_001054"
     CDS             complement(41366..42274)
                     /locus_tag="ACHK3A_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFO8739170.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELLWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(42279..42626)
                     /locus_tag="ACHK3A_001055"
     CDS             complement(42279..42626)
                     /locus_tag="ACHK3A_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO8739171.1"
                     /translation="MTARYLGMNRSNGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(42623..43258)
                     /locus_tag="ACHK3A_001056"
     CDS             complement(42623..43258)
                     /locus_tag="ACHK3A_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO8739172.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITSTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            43342..44127
                     /locus_tag="ACHK3A_001057"
     CDS             43342..44127
                     /locus_tag="ACHK3A_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739173.1"
                     /translation="MFSDNVINAWWFISLYLFLLIALTFVTFGKSNLMRFIAHHFNLE
                     YSDRKLKMLDKKWRDIQLFKTINGINVSGIEDVRMIQQGLIDGKLKTSYFFLTRFWGD
                     ITKPPHIIKTIIVILASIIYILFACYIHNKQSAIVRDAIGIPYKNMMYYVYSDKVLLS
                     FKNKTVELNKTYSLADCKTLQNVFIKDTLPEIACNKLLQLNEEDSEWLSQEIKDNNSH
                     KKALLILSLVYFISGLLIFLSYTKFLYANKKVLEYKASNKNHS"
     gene            complement(44199..44651)
                     /locus_tag="ACHK3A_001058"
     CDS             complement(44199..44651)
                     /locus_tag="ACHK3A_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO8739174.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSTELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEETILAHLER"
     gene            complement(44644..45111)
                     /locus_tag="ACHK3A_001059"
     CDS             complement(44644..45111)
                     /locus_tag="ACHK3A_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8739175.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSMSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(45074..45319)
                     /gene="lysC"
                     /locus_tag="ACHK3A_001060"
     CDS             complement(45074..45319)
                     /gene="lysC"
                     /locus_tag="ACHK3A_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFO8739176.1"
                     /translation="MLCAGCTSARPAQTPVIVHNACPKVSLCPMPGSDPQKNGDLSAD
                     IRLLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(45219..45644)
                     /gene="lysB"
                     /locus_tag="ACHK3A_001061"
     CDS             complement(45219..45644)
                     /gene="lysB"
                     /locus_tag="ACHK3A_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFO8739177.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASEQQ
                     TTITMLKNQLHVAITRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACADASDCPQRLPESESLPDAGQ"
     gene            complement(45632..46057)
                     /locus_tag="ACHK3A_001062"
     CDS             complement(45632..46057)
                     /locus_tag="ACHK3A_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFO8739178.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVATWAILATVIC
                     VVAGGVGVYATEYVLELYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRKACRQRLEGRELCQS"
     gene            complement(46072..46569)
                     /locus_tag="ACHK3A_001063"
     CDS             complement(46072..46569)
                     /locus_tag="ACHK3A_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO8739179.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(46569..46850)
                     /locus_tag="ACHK3A_001064"
     CDS             complement(46569..46850)
                     /locus_tag="ACHK3A_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO8739180.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(46854..47057)
                     /locus_tag="ACHK3A_001065"
     CDS             complement(46854..47057)
                     /locus_tag="ACHK3A_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO8739181.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(47057..47566)
                     /locus_tag="ACHK3A_001066"
     CDS             complement(47057..47566)
                     /locus_tag="ACHK3A_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO8739182.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(47666..48409)
                     /locus_tag="ACHK3A_001067"
     CDS             complement(47666..48409)
                     /locus_tag="ACHK3A_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFO8739183.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDIRLLTETLELTATAD
                     MPDEVRAKLHKITGLFLRDGGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRVPRKTRSVTPAKRGRPKKKAS"
     gene            complement(48413..49486)
                     /locus_tag="ACHK3A_001068"
     CDS             complement(48413..49486)
                     /locus_tag="ACHK3A_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO8739184.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            complement(49545..50399)
                     /locus_tag="ACHK3A_001069"
     CDS             complement(49545..50399)
                     /locus_tag="ACHK3A_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO8739185.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVVELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHDAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            50573..52345
                     /locus_tag="ACHK3A_001070"
     CDS             50573..52345
                     /locus_tag="ACHK3A_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFO8739186.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            52345..53379
                     /locus_tag="ACHK3A_001071"
     CDS             52345..53379
                     /locus_tag="ACHK3A_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8739187.1"
                     /translation="MSKKKGKTPRPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTPFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDND"
BASE COUNT        12233 a        14679 c        14026 g        12854 t
ORIGIN      
        1 taaataatgt tttattatta ccacttttaa ttcagggata atgtgaggtt ataaacctca
       61 aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta tccatgatcg
      121 aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat ggattgccga
      181 tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc tcggcattga
      241 taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaagcagc gcgaccgcgc
      301 acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg cgtcaatctc
      361 ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct ttagcgcccg
      421 cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga tggagctgaa
      481 cgaacggctg gaagggaaag acagcaataa ccccacccaa cgcaaaggtg cgaagttctg
      541 gggggtggtg acacaaattg tggtactgga cgccatcttc tcactcgact cggtgattac
      601 cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta tcgctatcag
      661 cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc cgaccatcgt
      721 tattctctgc ctaagtttcc tgttgatgat tggctttagc ctggtggcag aaggtttcgg
      781 cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga tgatcgaggc
      841 gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc agacactgcg
      901 ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag atgcggaact
      961 ggacgccgaa accgcgtcta tgctggtgga tcatggtaac cagcagatct ttaatccgca
     1021 ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtaccgtca gcagcattat
     1081 gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagaag aaatccgcca
     1141 gttactggag cgaaatcagc atacgcggct tgtcgttact gatggcgatg acgcagaaga
     1201 tttgctcggt gttgttcacg ttatcgacct gttacaacag tctctgcgcg gcgaaccgct
     1261 caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc cgttgttacc
     1321 tgccctggag cagttccgta atgcccgcac acactttgct tttgtggtgg atgagtttgg
     1381 ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgccg gtaacttacc
     1441 gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg acggttcctg
     1501 gacggcgaat ggtcatatgc cgctggaaga tctggtgcaa tatgtgccgc taccgctgga
     1561 tgagaaacgc gaatatcaca ccattgccgg gctgttgatg gaatatttgc aacgtattcc
     1621 aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc aggtggaaag
     1681 ccatcgcgtg cagaaggtgc agattattcc gctgcataag gatggcgaga tggagtacga
     1741 ggtgtgataa aacatcaaat gcgcaggttc aaactgcgca tttgatttat acaaaattta
     1801 gtataatcta aggatggagt aatgagaaaa aagctggctt ttcttgatac cagtcttgac
     1861 gatttacgcg catttccaga atcgtctcga caggaaattg gctatcaact cgacaggatc
     1921 caacaagggt taaacccata cgactggaaa cctttttcaa ctatcggccc tggtgtgcga
     1981 gagattcgta ctcgcgatgc tgatgggatc tatcgcgtca tgtatgtcgc aaaatttgaa
     2041 gaagcggttt acgtattgca ttgttttcaa aagaaaacac agactaccag tcaatcgaat
     2101 attgatttag ccaaacgacg ctataaagaa cttgttcagg agcggaaaaa tgaaaattga
     2161 aacctttgat agtgtgtggg atgcggtgag tgacacccct gaacaagcgg aaaatatgcg
     2221 gatccgtgcc gaacttgtta cgatcattaa taactggatt gaacaacaag ggtttagcca
     2281 ggctcaggca gcgtctgctt taggggtcac tcaacctcgc atatctgaac ttgccagagg
     2341 taagatccag atctttagta tcgataaatt gatcaccatg atggctcatg caggattgca
     2401 tattcaacgt attgaagttc aatatccaca tgctgcttag tactataaat acagatggcg
     2461 acaaattgac gccgccatct gcataacttg tcttacatct tctccagcaa cttcttcaca
     2521 tctttgccat tgcgggaatc tttattccgc tcggcccagt cattcaggcg acgtttcgct
     2581 tcgtcctgta gatgtttgcg cagtaactga tccacttgca ggctgtaatt aagttgctgc
     2641 cagttgccat aaacccgcag cggtactggc gtttctttga ggaaatcaat cagtttgctt
     2701 tccccgttcc aaccgccaat cacacgaatg tcaaactggg tgtcgcaggt ttgatctgcc
     2761 agattcaaca tgccttcccc tgtcagcgcc agcacaggcg attgaccttg catgtcgttt
     2821 aacgtcacga cgccatcctt caacgtcaaa tcggtggtaa agcggtcaag acgcgttacg
     2881 ttatcgaagt tttcagcggc cttcacatca ccaccattac gttctaccgc ttgctgaatc
     2941 atctgctgga agttcatccc ttccatgcgc gtgtcggtca tttcgacatg tgcctgtcct
     3001 tgccagttat ggcggaatgc gtcggcatct atgtcagcac cggagaaatc accagccagt
     3061 gacatctttc cggtcaacga aatcggatag ttaaacgcct tcagaatggt gccaatctca
     3121 acgttttcca gccgtggctg gaagtttatt cgcggattta ttgatgtcgc gtccagcgtg
     3181 cccggcagtg aaacctgtcc accgttaagt ttgccctgca gttgagtaat ttccagcaga
     3241 cccgacttgt tggtcatttg cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg
     3301 ttactggcct gcaacaaaat atcagccgta aagccttgca gtccctgata ggccggttca
     3361 tcaatacgcg aagaaatgac cgggcgcggc aacgtggatt ggctctgccc ctgctgcgcg
     3421 gcaccgtttt caccgttcgc tgtttcttta agcgggatga ggttgtcaag attcagttgc
     3481 gggaattgca gcctcagctg ccattccggt ttctctgtca gcgtgacctg tgcttgcccg
     3541 ctcagcgtac tgtcattggc ggtcaagctg atttggttaa atgaaaggcg tttatgtgac
     3601 tcctgccact gggcctggaa actcccctgc ccctgaatac cttgttttgg taaatctgca
     3661 ccctgcaact gccagttaac ttgttcaata gccgccgtta aatcatgcgg ataatcagaa
     3721 gcatctaccg taccgttaag ggatatcgtg agatcgcgct gatcgcgatt aacccgcccg
     3781 gagaactcaa atgaaccacg atgttgggga tcttgttcca tttgcaggcg aatattgcgg
     3841 attgtcacct gctcgtcatc ttcatgctgg aacaccagca cgctgtccgc caccttaaga
     3901 ctggatatat caaacgacca tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc
     3961 ggtgcaaccg gagcgtcttc actgcgcacc gcttccgtct gcggcgtcag ttggatcact
     4021 gcccctttta gcatcacctg cttaacgctc agttgatgac tcagcagtgg taaaagcgcc
     4081 acgtccagac gcatgttgtc tgcgcgaacc agtggctggc tcgcgccctg ggcagtgaga
     4141 gacattcgcc cggagaggat actaagctgc ggccagacgt gccaacgcag tggcccgtcg
     4201 agctgcaatt gataaccgct acgtgcagca acttgcttga ccatatagtc gcggaaatca
     4261 ttcggattca ccagcaacac taacgcagat aacccggcca ccagcacgac caggagtatc
     4321 atcagcgtcg tcagaaatcg tctcatggta tcctcaatgg gcctgaatta gtctttatcg
     4381 attcgactgg ctaccgcgcc ctgctggttg cgatatttcg catcttcacg gcggttgtaa
     4441 ggtcgcgccg ccgggccgga aagcggctca aagctcagcg caccaattaa catgcccgga
     4501 cgcagcgcca gcggcagctt accggagttg tagaactcca gcacaataca gccagaccag
     4561 cccggatcga tacggtgcgc ggtgacgtgc accataagcc ccagacgcgc cagtgaggaa
     4621 cgaccgtcca gccatcccac cagatcggct ggcagcgtca ccgactccag cgtcaccgcc
     4681 agcgccagct ctcctgggtg aagataaaac gcctcgcttt catcgagtac gatctcatcg
     4741 ctcatcacgc ggtcaagcgc ggcgctcact tcatctttgg gaccgctcag atcgataaac
     4801 gcagccgtgt gaccacggaa ggtacgaaat ttattgccca ggcgcacatc caccgtcgcg
     4861 ccgttaatac gctccactgg cggacgtggg ttgatcgaca aacggccttc atcaagccag
     4921 gcttcaatat ctcggtcaca cagacgcatg gcattttctc ctttcgcgca tcactccctt
     4981 aacgccaatt acgtcaaggg cataccaggt tatcactgaa cggtacacaa tttatcaagc
     5041 ttattcaaag aactgactta ttttcgcttt caatatatcg atggcgatgc ggtttttccc
     5101 gccgcgcggc acgataatgt ccgcatattg tttagaaggc tcaatgaatt gcaggaacat
     5161 cggacgcacg gttttttgat attgcgccat cactgaatcc attgaacgcc cacgctcgtt
     5221 aacgtcacgc ttgatgcggc gcatcaggca gatatccagc ggggtatcaa cgaaaatgga
     5281 gaagttaagt tcatcacgca aacgcgcatc cgtcagcagc aaaatgcctt cgagaatgat
     5341 gactttcttc ggctcaaccg tcaccgtttc tttcatacgc gtatgttcaa catagctgta
     5401 aaccggcagg tcaatggccg agccgcgttt caacgcttgt aaatgctcaa gcaacaggct
     5461 gtgatccata gcgctgggat ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg
     5521 gctttgatct ttgtaatagc agtcttcggg aattacgccg atgtgttcat caccgacttg
     5581 ctcacgtaat tcacgataaa gggtactggc aataagactc ttgccggaag ccgatgcgcc
     5641 agcgataccg ataatgacgc actgatgaga ctgatcagtc atatatttag cgacctgatt
     5701 aacctggatg ttaggaaggg ggcgacgaag cgccaaacgc ggcaattata gggatttcat
     5761 ccgcctgata ccagtcgaat agcgttgccg cgcgctcaga gttaattgtt gacgaagaat
     5821 tcccggtggc aaattacgtt gatcagtttt atacaaggta aaaattgtta tacgcagttg
     5881 cgcaaattat ccgcctttac gtcactttat gagcaattcg catataaaat gtaaaacttt
     5941 tgtactagca taaacacaga aacgaatact ggcgacctgg tcttgcggat aaagcggtaa
     6001 tgagcaaaca atcacagcat gtattaattg ccctgcccca cccgctgctt cacctggtca
     6061 gtttaggttt agtctcgttt atctttaccc ttttctcgct tgagctttcg cagtttggca
     6121 cccaactcgc cccactgtgg ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg
     6181 ccgggcgcat gtggccgggg attgcgctga gctgctcgct gggaaatatc gccgcatcca
     6241 tcctgctttt ttccaccagc tcgctgaaca tgacctggac gaccatcaat attgttgaag
     6301 ccgtggtcgg ggcagtgcta ctgcgtaaat tgctgccgtg gtataaccca ttgcaaaatc
     6361 tggctgactg gctgcgtctg gcactcggca gcgccattgt tccacctctg ttagggggtg
     6421 ttctggttat cctgctgacg cccggagacg atcctctcag ggcatttttg atatgggtac
     6481 tgtcagaatc catcggcgca ctggcactgg tgccgctggg attgttattt aaaccacact
     6541 atctgctgcg ccatcgcaac ccacggttgc tttttgagtc gctgctcacg ttagccatca
     6601 cactgacgtt aagctggctt tcgatgctgt atctgccgtg gccttttact ttcattattg
     6661 tgctgttgat gtggagcgcc gtgcgcctgc cacgaatgga agcctttttg atcttcctta
     6721 ccacggtgat gatggtgtca ctgatgatgg ccgcggatcc ctccctgctt gctacgccgc
     6781 gtacatacct gatgagccat atgccgtggc taccgttttt gctgatcctg ctgcccgcca
     6841 acatcatgac catggtgatg tatgcctttc gtgcggaacg caaacacatt tccgaaagcg
     6901 aaacccgctt tcggaacgcg atggaatatt ccgccatcgg catggcatta gtgggcaccg
     6961 agggacaatg gctgcaatcc aacaaagcgc tctgccagtt tctcggttac agtcaggaag
     7021 agctgcgcgg actcaccttt cagcaactga cctggccgga ggatctcaat aaagatctgc
     7081 aacaggttga aaagctgatc agcggtgaaa taaacaccta ttcaatggaa aaacgctact
     7141 acaaccgcaa tggcgatgtt gtctgggcgt tgcttgccgt ctcactggtg cgccacaccg
     7201 atggtacgcc gctctatttt atcgctcaga ttgaagacat taacgagcta aaacgcaccg
     7261 aacaggtgaa ccagcaactg atggagcgca tcacgctggc taacgaagcg ggcgggattg
     7321 gcatctggga gtgggagcta aagccgaata tttttagctg ggataagcgg atgttcgagc
     7381 tgtatgaaat tcctccgcat atcaaaccga actggcaggt gtggtacgag tgcgtactgc
     7441 cggaagatcg ccagcatgcc gaaaaagtga ttcgtgattc gttgcaatca cgctcgccct
     7501 ttaagctgga atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca
     7561 accgggtact gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga
     7621 ccgaagtgaa acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc
     7681 tggattccat cggcgaagcc gtggtctgta ttgatatggc aatgaaaatt acctttatga
     7741 atccggtggc ggagaagatg agcggctgga cgcaggaaga agcgttaggt gttccgctcc
     7801 tgacggtgtt gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg
     7861 ccgacacctc acgttccgcg attgaacaag atgtggtgtt gcactgtcgg agcggcggca
     7921 gctacgacgt gcattacagt attacgccgt taagtactct ggacggcagc aatataggct
     7981 cggttctggt gattcaggac gtcaccgaat cgcgtaaaat gctgcgccag ctgagctaca
     8041 gcgcctccca tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactac
     8101 gcatcctgct gcaaacggta aacagtacac atcagcgaca tgcactggtg tttatcgatc
     8161 ttgatcgctt taaagcggtg aatgacagcg ccgggcacgc cgctggcgac gctttactgc
     8221 gcgaactggc gtcattgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg
     8281 gtggcgatga atttggtctg ctgctgccag attgtaatgt cgaaagtgcg cgttttatcg
     8341 ctacacgcat tatcagcgcc gtgaatgact atcactttat ctgggaagga cgtgtacatc
     8401 gggtaggtgc cagtgccggg attaccttga ttgatgacaa caatcatcag gcggctgaag
     8461 tgatgtcgca ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga
     8521 cggtttacga accgcagcaa gctgccgcac atagcgaacg ggcggtgatg tcgcttgatg
     8581 aacagtggcg gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc
     8641 cgcggatccc ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag
     8701 gcgagattat tgatgaacaa acatttcgtc gtagcttcag cgatccggca cttagccatg
     8761 ctcttgaccg acgggtattc cacgattttt tccagcaggc cgcaaaagcg attgccagta
     8821 aaggcttaag catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga
     8881 atgaactgct tgagcagctg gaaaatagcc ctctaccccc acggttatta catctgatta
     8941 ttccggcaga cgcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat
     9001 gtcggatcgt attcagtcag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga
     9061 atatggcaga ttacctgcta cttgatggtg agttatgcgc caatgtgcag ggaaatttga
     9121 tggatgagat gctgattacg atcattcagg ggcacgctca gcggctcggg atgaagacca
     9181 tcgccgggcc agtagtttta cccctggtga tggatacgct ttctggcatc ggcgtcgatc
     9241 tgatttatgg cgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt
     9301 atttcgcgat taactgaggc gtgcttctcc atcgcctgat acgacgctaa cgcgtcttat
     9361 catgcctaca aatcgctcat tccccaggcc ggataaggcg ctcgcaccgc atccggcgac
     9421 caacgtcatg cttcgtctgg ttgccagcct tccgtatacc agatatgcaa cagcgcataa
     9481 gaacgcaagg gcttccagcg ttcggcataa cggcggattt gtgccggtgt cattcccgga
     9541 aatcgctgtt taatcagata atcatccggc aggaaaacat ctttcgcctg ccagccgcgc
     9601 aaggcgaaat aattcgccgt ccagcgcccg atacccggaa aagtttgcag cgttttcatc
     9661 gcctgctcca catcgcccgg tattgtcatt ggtaaggtgc cctccagcgc cgcatttgcc
     9721 agatgaatca gcgcctctgc ccgtttcaac ggcataccta acgcttttaa tgcctgcgga
     9781 tctgctgctg ccagccgctg aggcgtgggg aagcagacat aatccggaaa atcatccagc
     9841 ctttcgccat aaagctgtgc cactctggcg gtcaattttg ccgccatcgc cacgctcacc
     9901 agctggccta aaatcgcccg cacgccctgc tcaaaagcat caacacaacc gggtaaacgc
     9961 aatccgggcc gtgccgcgcc taacttgccc aacgcaccgt taacaatctg cgggttacat
    10021 tgcagatcaa acaggcggct cattttcgcc agacactctg cggcaacggg ttctaaacct
    10081 gcacttaaat ttatgtgcag agtatggctg gctatatccg gaatagcagt caccacgccg
    10141 cgacattcgc ccaccgccag actacgggca taataatcgt cagcgaccgt ttccacaccg
    10201 ttcaccgcac gggcggcgag aaatcccaac atccacgacc agtcatacgg cggctgccag
    10261 ttcagggtat acatcgcatc tccttgttaa tccgctttca gcataaacgt tattcagacg
    10321 ctacgctttg ctttcatatt ccggttgtcg cgacggcaac atttcgctaa agtcacgccc
    10381 cttcttcacc ggcatgggga ttatttcgtg tttattggtt ttgattacgg tacagcaaac
    10441 tgttcagtgg cggtcatgcg cgacggtaag ccgcaattac tgaaaatgga aaacgacagc
    10501 acgctgctgc cttcaatgct ttgcgcgcca acgcgtgaag cggtaagcga atggctgtac
    10561 cgccatcatg atgttccggc agacgacgat gaaacgcagg cgctgttgcg tcgggcgatt
    10621 cgttataacc gcgaagaaga tatcgatgtt acggcgaaaa gcgtgcagtt cggtctttcc
    10681 tcactggcac agtacattga tgatccagaa gaggtgtggt ttgtgaaatc accaaaatca
    10741 ttcctcggtg ccagcggctt aaaaccgcag caggtagcgc tgtttgagga tctggtctgc
    10801 gcaatgatgt tgcacattcg ccagcaggcg caggcgcagc tgccggaggc aattactcag
    10861 gcggtgattg gtcgcccgat caacttccag gggctgggcg gcgatgaagc aaacgcccag
    10921 gcgcaaggga ttctggaacg cgcggcgaag cgtgccgggt ttaaggatgt ggtattccag
    10981 tacgagccgg tcgcggctgg gctggattac gaagccacct tgcaggaaga aaaacgggtg
    11041 ctggtggtgg atatcggcgg tggtacgact gactgttcat tgctgctgat ggggccgcag
    11101 tggcgttcgc gtctcgatcg tgaagccagc ctgctgggtc acagtggttg ccgtattggc
    11161 ggtaacgatc tggatatcgc actggcgttt aaaaacctga tgccgttact gggcatgggt
    11221 ggcgaaaccg aaaaaggcat cgccctgccg attctgccgt ggtggaatgc ggttgccatc
    11281 aacgacgttc ctgcgcaaag tgatttctac agtagtgcca acggtcgtct gcttaacgac
    11341 ctggtacgcg atgcccgcga accggaaaaa gtggccctgt tacagaaagt ctggcgtcag
    11401 cgtttaagct atcgcctggt acgtagcgca gaagagagca aaattgcgct ttcaagcgta
    11461 gcggaaaccc gcgcctcact gccgtttatc agcgatgaac tggctacgct gattagccag
    11521 caagggctgg aaagcgccct cagccagcca ctggcgcgga ttctggaaca ggtgcaactg
    11581 gcgctggata acgcccagga aaaaccggat gttatctatc tgaccggcgg tagcgcccgt
    11641 tcaccgctga ttaaaaaagc gctgacagaa cagttgccgg gcattccgat tgcaggtggc
    11701 gatgactttg gctccgtcac cgccgggctg gcacgctggg cggaagtggt gtttcgttaa
    11761 ttgaaaactg gctcagcaac ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc
    11821 ctgacgtagc ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg
    11881 caactctgtc gcaccgacat tcagcgccga ttccagccga tgtcgtttgg cggccatttt
    11941 tgccattacc gcttgtctgt ctgcaggcgt taccgcttcg ttcggcctga acacaaaacg
    12001 gcgttcacag ctcgctttcc agtcgataac cgcctgggtc agatgatcac caaaaccttt
    12061 cacttgctta acgctccgtc gggtaacatc cgctgctgtt tcaataccaa aggaccgtaa
    12121 cgccgcttta cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc
    12181 cagaaacttc tgcttctgcc gttcccttgc ggtgtcctga agtgctgcca gatcgcgctt
    12241 ttcttcttcc ggtaacccaa gaatttcgtc cttcattttt tcgagcatcg tccgtttggc
    12301 gataaaacct tccagcccgc ccaactgctg gatctggctg actaaatggt catagtccat
    12361 tttcgcgcga ttaaacgcct cacggcgttg ctggatttct gctttgtacg ctttgcttgt
    12421 caggctgcca ataatccaga tagccgccag cacaggaacc aaaataatat aacgtggttc
    12481 tgtctgaagg ccgcaaagca gcagcgataa cgctgacagt gcgatctcaa tcagaatgat
    12541 gtattcacgc cttaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg
    12601 taatggtaat tgcaatgctg gcggtgctac tgacgccatc accatcgccc agactttcgc
    12661 cagcacaaaa tcaccgctgg tggtaatgac ctcttcgccg agatcaataa aatagataac
    12721 gccttgatta tccagagcac accacgggca gtcagccaaa tgaccgggat aaacatgcat
    12781 tgccgaaacg gtacatttct ttaactgttg gcgtagtaaa tcaagtgccg ctacccacgc
    12841 ttttgccgtc ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc
    12901 ttcaacatgc ccggtaacat cgacagcgga atagatcgtg gcggcggttt taaaccacgt
    12961 cgctgattat ctgacgcgta ggcataacgg aaatgggcaa tatccgtctc cagtgcatta
    13021 cccgcatcag agacaagcgg tacaccggaa taaggatgcc gcccaccaaa caagacgtga
    13081 aaaatcagca acgcaaggcc aaaattatcg tgattcgcgg tgcgttcaaa gccgacaaat
    13141 gatggcaagg tttgttgctc tggcggcgta aaatgcgaca cgccgacttc gcataaatgc
    13201 agcgtgccat tggcgttaat ctgaaaggag tcgctatcaa tcaacaccac tttgctgtcg
    13261 cgacctacca taaagctgtt ctggtttacg tcacccacga cgtgcccgtg ctcgtgaatc
    13321 gtagcaaaag atgaagcaat attgcgcgca acatagagta gaaaatccca cgcacaatga
    13381 gggtaacgtt gacgacgatg tgccgggcta tagatcatat gaatcggttc tttaccagaa
    13441 acttttggca tcataaaacc gattactttt ccgcctcgtc caccgtgaag cgttgcctgc
    13501 ggccaggcga cataattcaa caactgcgcg tcagctgtcg cagccataaa ggcaagtttg
    13561 tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg cgacgctatc gacaaactcc
    13621 tcgatatcat aaaccgcgcc ttcaccgcct ttgcccagtt cacagcctgg cgtaacgcac
    13681 tcaccagtag cagtatataa agtgggtttc atggtaacca cagcgccagt gccaacgttt
    13741 tatcatcatc ggtacgctcg tttaccgccg ggccggacaa aaactgcttt aacaattcag
    13801 gtaataaatc gagttgttcc tgcgttgctg acgccagccc attgaaaaac ggggtaaaga
    13861 aaggcacatg cggagaatta tccagcatat ttaacgccag ccgctggata ccatccgtaa
    13921 atgctgcaac tttgtgcgca cgctcagtgc tggtgaaagt ttccagtctg gaaacggcat
    13981 cttcatcggt aatgaagtgc gtcatattgg tatattcacc caccatcggc gtgagcggca
    14041 gttgcaaacc gtgccccaaa tctaccacca cgccgccatc gccaatctgc ataatcaagg
    14101 tgccattagc tgatgaaatc aatcccaaaa acgtgcaggc aaaatcgcga accgctaatt
    14161 ctttggcttc cgcctcggca aaaagtcgct gacgaatggt cagcacaatg tccgttgcga
    14221 gaacatcatt aagccccaat tccccgccct gcactttttg cgacatataa gccatcgctt
    14281 cattgacggc gagcatcgca ccttcgcctc cctgcgaggc gctacctgcg ccatcagcga
    14341 caaataccga caataatggc tgctggtcat ttaaccaggc aacctgcatc tggcaggcat
    14401 cctggcaagg caagtccgcg ctgatgtgcg acgttccaac ggttgatgca tagaccagac
    14461 gccagctcac actgacgtcc agccttttgg cgcttccagc acaacttccg ttcccggcgt
    14521 ggagcgggaa accgaacgca gcgaactgga taaccagcta aatagttcgc ggaattgtaa
    14581 tccttgaaga ggcaaaggct gacgaacgct gatttgggcc agcgtcttca tatccgcccc
    14641 ctgaacaccg atggaaaaga aggcaaactt cttatcttct tccccctgaa acaccttgtt
    14701 ggcagcagcc tgccactcat cggttggtgc gccatcggta atcaggaaaa tccacggacg
    14761 ataataggaa ataccattgg cgcgatactc acgtttgcgc tcctcaacca tatccagggc
    14821 tttggtaatg gcggccccca ttggtgtatc gccctgggca aacaggatgg gcgggaaaaa
    14881 attagcggcg ctggtaaatg gttgttccac atgcaccggg ccgaacgtca caatgccgag
    14941 ttccactctt tttaaggcca gcggatcggc aagcagctca tcgcgaaagg taaccaatcc
    15001 ggcgttaagt tcattaatag gtctaccgtt catagaacca gaaacatcca acagcaaaat
    15061 gcaggggcaa cgtggttccg ggttactggc aaaatcgctg gtggcaaacg tgatttgttc
    15121 gctcataaga aatcctttct tagagaatta ttgaggcccg gtatcatacc gactgcaaca
    15181 atgaaataag agcaaacgaa gaaactttca gaattaggcc cggcggcgca gaaaattttg
    15241 ctattcgcct cgcaagaacg gagagccgtc gattgacact aagggcggag tgacataatt
    15301 tcaggagtga gggttaggga gaggtttccc cctccccctg gtgttcttag taagcctgga
    15361 agctaatcac taagagtatc accagtatga tgacgtgctt catcataacc ctttccttat
    15421 taaaagccct cttctccggg agagggtttc ccgtttcagc gtcccgctga aatcgtcggc
    15481 ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg tgaacaaaat agtttcctct
    15541 gtgattattg cgtaacgact cgcaaagaat atttgctggc aggatcgcag actacaaagc
    15601 ctgcggattg acaatcttat cgtgaaggca taatttcagg agtgagggta gagcggggtt
    15661 tcccccgccc tggtagtctt agtaagctgg ccagctaata actaagagca ccacgatgat
    15721 gagtagcttc atcatgaccc tttccttatt taaggcccct tcctcgggag gggctttccc
    15781 gtttcagcgt cccgctgaaa tcggcggcct acctcctttc gccatgcaag cagtctatcg
    15841 ctaacttgca gataaaatag tttcacatgt gattattgcg taacgtctcg caaagaatat
    15901 ttgctggcag gatcgcagac tacaaagcct gcggattgac aatctaattg tgaaggcata
    15961 ctttcaggag tgagggtaga gcggggtttc ccccgccctg gtagtcttag taagccggga
    16021 agcttatgac taagagcacc acgatgatga tccacttcat catgaccctt tccttattta
    16081 aggccccttc ctcgggaggg gctttcccgt ttcagcgtcc cgctgaaatc atcggcttac
    16141 ctcctttcgc cctgaatgca gtctatcgct gacttgcgga taaaatagtt tcacatgtga
    16201 ttattgcgtg acgtctcgca aagaatattt gctggcagga tcgcagactg caaagcctgc
    16261 ggattgacaa tcggaccgtg aaggcatact ttcaggagtg agggtagagc ggggtttccc
    16321 ccgccctggt agtcttagta agctgggtag ctaacgacta agagcaccac gatgatgagt
    16381 atcttcatca tgaccctttc cttatttaag gccccttcct cgggaggggc ttgcccgttt
    16441 cagcgtcccg ctgaaatcgt cggcctacct ccttttgccc tgaatgcagt ctatcgctaa
    16501 cgcgtagata aaatagtttc ctgtgttatt actggatggg tgctcgcaaa tgtgcccgcc
    16561 attcagacga ttcccgatag tgtttcataa ttcctccatt tttctccctg attggctggc
    16621 tacactagta tcattccgcg aatcgtttca ggaagagaaa ctcttaacga tgaaaagcag
    16681 ttataaatcc cgttgggtaa tggtaatcgt ggtggttatc gccgccatcg ccgcattctg
    16741 gttctggcaa ggccgcaatg actcccggag tgcagcgcca ggggcgacga aacaagcgca
    16801 gcaatcgcca gcgggtggtc gccgtggtat gcgttccggc ccattagccc cggttcaggc
    16861 ggcgaccgcc gtagaacagg cagttccgcg ttacctcacc gggcttggca ccattaccgc
    16921 cgctaatact gttacggtgc gcagccgcgt ggacggtcaa ctgatggcgt tgcatttcca
    16981 ggaaggccag caggtcaaag caggcgattt actggcagaa attgacccca gccagttcaa
    17041 agttgcatta gcacaagccc agggccaact ggcaaaagat aaagccacgc ttgccaacgc
    17101 ccgccgtgac ctggcgcgtt atcaacaact ggtaaaaacc aatctcgtat ctcgtcagga
    17161 gctggatgcc caacaggcgc tggtcagtga aaccgaaggc accattaagg ctgatgaagc
    17221 aagcgtcgcc agcgcgcagc tgcaactcga ctggagccgc atcaccgcac cagtcgatgg
    17281 tcgcgttggt ctcaagcagg ttgatgttgg taaccaaatc tccagtggtg ataccaccgg
    17341 gattgtggtg atcacccaga cgcatcctat cgatttgctc tttaccctgc cggaaagcga
    17401 tatcgctacc gtagtgcagg cgcaaaaagc cggaaaaccg ctggttgttg aagcctggga
    17461 tcgcaccaat tcaaagaaat taagtgaagg cacgctgtta agtctcgata accaaatcga
    17521 tgccactacc ggtacgatta aagtgaaagc acgcttcaat aatcaggatg atgcgctgtt
    17581 tcccaatcag tttgttaacg cgcgcatgtt agtcgacaca gagcaaaacg ctgtggtgat
    17641 cccaacagcc gccctgcaaa tgggcaatga aggccatttt gtctgggtgc tgaatagcga
    17701 aaacaaggtc agcaaacatc tggtgacgcc gggcattcag gacagtcaga aagtggtgat
    17761 ccgcgcaggt atttctgcgg gcgaccgcgt agtgacagac ggcattgatc gcctgaccga
    17821 aggggcgaaa gtggaagtag tggaagccca gagcgccacc actccggaag agaaagccac
    17881 cagccgcgaa tacgcgaaaa aaggagcacg ctcctgatgc aggtgttacc cccgagcagc
    17941 acaggcggcc cgtcgcgcct gtttattatg cgtcctgtcg ccaccacgct gctgatggtg
    18001 gcgatcttac tcgccgggat tatcggttat cgagccctgc ccgtttcggc gctgccggaa
    18061 gtggactatc cgaccattca ggtagtcacg ctctacccag gtgcgagccc ggatgtcatg
    18121 acctctgccg ttaccgcgcc gcttgaacgc cagtttgggc agatgtctgg cctgaaacag
    18181 atgtcgtcgc aaagttccgg cggtgcgtca gttatcactt tgcagttcca gctaacatta
    18241 ccgctcgatg tcgccgagca ggaagtgcag gccgcgatta acgctgcgac caacttgttg
    18301 ccgagcgatc tgcctaaccc gccggtttac agcaaagtga acccggcaga tccgccgatc
    18361 atgacgctcg ctgtcacctc aaccgccatg ccgatgacgc aagtggaaga tatggtggaa
    18421 acccgcgtcg cgcagaaaat ctcgcagatt tccggcgtcg gcctggtgac actttccggc
    18481 ggtcagcgtc cggctgttcg cgtcaaactt aacgctcagg cgattgccgc cctcggcctg
    18541 accagcgaaa ccgtgcgcac cgccattacc ggcgctaacg tcaactcggc aaaaggtagc
    18601 ctcgacggcc cttctcgtgc ggttacgctt tccgctaacg accagatgca atccgccgaa
    18661 gagtatcgcc agctaatcat cgcctaccag aacggcgcgc cgattcgtct gggcgatgtc
    18721 gcaaccgtag agcaaggtgc agaaaacagc tggcttggcg catgggctaa caaagaacag
    18781 gccattgtga tgaatgttca gcgccagccc ggtgctaaca ttatctccac cgccgacagc
    18841 attcggcaga tgctgccaca gctcaccgag agtctgccga aatcggtgaa ggtgacggta
    18901 ctttctgacc gtaccaccaa tattcgcgca tccgtggatg atactcagtt tgaattgatg
    18961 atggctatcg cgctggtagt catgattatc tacctgtttt tgcgtaatat tccggcgacc
    19021 atcattcccg gcgtcgcagt gccgctgtcg ttaatcggca ccttcgcggt catggtgttt
    19081 ctcgattttt caatcaataa cctgacgctg atggcgttaa ctatcgccac cggatttgtg
    19141 gtcgatgacg ccatcgtggt gatcgaaaac atttcccgct atatcgaaaa aggcgaaaaa
    19201 ccgctggcgg cggcgctcaa aggcgcaggt gaaatcggtt ttaccattat ctcgctgacc
    19261 ttctcgctga ttgcagtgct gatcccgcta ctgtttatgg gcgatatcgt cgggcgactg
    19321 ttccgcgaat ttgctattac tctggcagtc gcgattttga tctcagccgt ggtgtcgctg
    19381 accctgacac cgatgatgtg tgcgcggatg ctcagccagg agtcgctgcg taaacagaac
    19441 cgcttctccc gtgcctcgga aaaaatgttc gacaggataa tcgccgccta tggtcaggga
    19501 cttgcgaaag tgctgaatca tccgtggctg accttaagcg tggcgctcag cacgctgctg
    19561 cttagcgtgc tgttgtgggt gttcattccg aaaggtttct tcccggtaca ggacaacggc
    19621 attattcagg gcactttgca ggcaccgcaa tccagctcct ttaccaatat ggcccagcgg
    19681 caacgccagg tcgcggatgt gattttgcaa gatccggcgg tgcaaagtct gacctcattt
    19741 gttggcgttg atggcactaa cccgtcgctg aacagtgcgc gtttgcagat caacctcaaa
    19801 ccgttggatg aacgtgatga tcgggtgcaa aaagtcatcg cccgcctgca aacggcggtg
    19861 gataaagtgc cgggcgtcga tctcttcctg caaccaacgc aggacctgac catcgatact
    19921 caggtcagcc gcacccagta ccagtttacc ttgcaggcca cgtcactgga tgcgctcagt
    19981 acctgggtgc cacagttgat ggaaaaactc cagcaactgc cacagctttc tgatgtctcc
    20041 agcgactggc aggacaaagg gctggtggcg tatgtcaatg ttgatcgcga cagcgccagc
    20101 cgtctgggga tcagcatggc ggacgtcgat aacgcccttt acaacgcgtt tggtcagcga
    20161 ctgatttcca ctatttatac tcaggccaac cagtatcgcg tggtgctgga gcacaacacc
    20221 gaaaatactc ctgggcttgc ggcgctggat actattcgcc tgaccagcag cgacggcggc
    20281 gtggtaccgc taagctcaat tgccaaaatt gagcagcgtt ttgcgccgct ttccatcaac
    20341 catctggatc agttcccggt aacgaccatc tcctttaacg tgccggataa ctattcgctg
    20401 ggcgatgcgg tgcaggcgat tatggacacc gaaaagacgc tgaatctgcc ggtggatatc
    20461 accacgcagt tccagggcag caccctcgcc ttccagtcgg cgttgggcag cactgtctgg
    20521 ctgattgtcg cggcggtggt ggcgatgtat atcgtgctcg gcattctgta cgagagcttt
    20581 attcacccga tcaccattct ctcgacgcta cccaccgcag gggtcggcgc actgctggcg
    20641 ttactgattg ccggtagcga gctggatgtg attgcgatta tcggcattat tttgctgatc
    20701 ggtatcgtga agaagaacgc catcatgatg atcgacttcg cgctggctgc tgagcgcgag
    20761 caaggcatgt cgccgcgcga tgcaatctac caggcttgtc tgttgcgttt tcgtccgatc
    20821 ctgatgacca ccctggcggc tctgcttggc gcgctgccgc tgatgttgag taccggggtc
    20881 ggcgcggaac tgcgtcgtcc gttaggtatc ggcatggtcg gcggtctgat tgtcagccag
    20941 gtgctgacgc tgttcaccac gccggtgatt tatctgctat tcgaccgcct ggcattgtgg
    21001 accaaaagcc gctttgctcg tcatgaagag gaggcgtaag tgaagttttt tgccctcttc
    21061 atttaccgcc cggtggcgac gattttactg tcggtcgcca ttaccctgtg cggcatactg
    21121 ggtttccgta tgctgccagt cgccccgctg ccgcaggtcg attttccggt gattatggtc
    21181 agcgcctcgc tgcccggtgc gtcaccagaa acaatggcgt cttccgttgc cacgccgctg
    21241 gaacgttcgc ttggacgcat tgccggggtc agtgaaatga cctccagcag ttcgctcggc
    21301 agcacgcgca ttattttgca gtttgatttt gaccgcgata tcaacggcgc agcgcgcgat
    21361 gtgcaagcgg cgatcaacgc cgcgcaaagt ttgctgccca gcgggatgcc aagccgtccg
    21421 acctatcgca aagcgaaccc gtcggatgcg ccaattatga tcctcacgct gacatccgat
    21481 acttattcgc agggtgaact gtacgatttc gcctcgacgc agctggctcc gacgatttcg
    21541 caaatcgacg gtgtcggtga tgtcgatgtt ggcggcagct cactgcccgc cgtacgcatc
    21601 gggctgaatc cgcaggcgct gtttaatcag ggcgtgtcgc tggacgatgt gcgcagcgcc
    21661 atcagcaatg ccaacgtgcg taagccgcag ggggcgctgg aagatgatac ccatcgctgg
    21721 cagatccaga ctaacgatga actgaaaacc gccgccgaat atcagccgtt gattattcat
    21781 tacaacaacg gcggcgcgct tcgtctgggc gatgtggcga cggtgaccga ctcggtgcag
    21841 gatgtgcgca acgccgggat gaccaacgcc aaaccggcta ttttgctgat gatccgcaag
    21901 ctgccggaag ccaatattat ccagacggtt gacagcatcc gggcaaaatt accggagttg
    21961 caggagacca ttccggcggc gattgatctg caaattgccc aggatcgctc ccccaccatt
    22021 cgcgcctcgc tggaggaagt cgagcaaacg ctgattatct cggtggcgct ggtgattctg
    22081 gtggtctttt tattcctgcg ctcgggtcgc gccactatta ttcccgccgt tgcggtgccg
    22141 gtttcgctga ttggtacgtt tgcggcgatg tacctgtgcg gattcagtct caataacctt
    22201 tcgttaatgg cgctcaccat cgctactggt ttcgtggtgg atgacgccat cgtggtgctg
    22261 gaaaacattg cccgtcacct ggaagcgggg atgaaaccgc tacaggccgc gctgcaaggc
    22321 actcgcgaag tcggttttac ggtgctgtcg atgagtctgt cactggtggc ggtgtttctg
    22381 ccgctgctgt tgatgggcgg attgccgggt cgcctgttac gcgagtttgc cgtgacgctt
    22441 tctgtcgcca ttggtatttc gctgctggtt tctctgacgc taacgccaat gatgtgcggc
    22501 tggatgctaa aagccagtaa gccgcgcgag caaaagcgac tacgtggttt tggtcgcatg
    22561 ttggtagccc tgcaacaagg ctatggtaag tcgctgaaat gggtgcttaa tcatacccgt
    22621 ctggtgggcg tggtgctgct tggcaccatt gctttgaata tctggctgta tatctcgatc
    22681 ccgaaaacct tcttcccgga gcaggacacc ggcgtgttga tgggcgggat acaggcggat
    22741 cagagtattt cgtttcaggc gatgcgcggt aagttgcagg attttatgaa aattatccgt
    22801 gacgatccgg cagtggataa tgtcaccgga tttaccggcg gttcgcgggt gaacagcggg
    22861 atgatgttta tcacgctgaa gccacgcggc gaacgcagcg aaacggcgca gcaaattatc
    22921 gaccgtctgc gcgtgaagct ggcgaaagaa ccgggggcga atctgttcct gatggcggta
    22981 caggatattc gcgttggcgg acgtcagtcg aacgccagct atcagtacac gttgctgtcc
    23041 gacgacctgg cggcactgcg cgaatgggag ccgaaaattc gcaaaaaact ggcgacattg
    23101 ccggaactgg cggacgtgaa ctccgaccag caggataacg gcgcggagat gaatctggtt
    23161 tacgaccgcg acaccatggc acggctggga atcgacgtgc aggccgccaa cagcctgtta
    23221 aataacgcct tcggtcagcg acaaatctcg accatttacc agccgatgaa ccagtataaa
    23281 gtggtgatgg aagtggatcc gcgctatacc caggacatca gtgcgctgga aaaaatgttc
    23341 gttatcaata acgaaggaaa agcgatcccg ctgtcgtatt tcgctaaatg gcaaccggcg
    23401 aatgctccgc tgtcggtgaa tcatcaggga ttatcggcag cctcgaccat ctcgttcaac
    23461 ctgccgaccg ggaaatcgct ctcggacgcc agtgcggcga tcgatcgcgc aatgacccag
    23521 cttggtgtgc cttcgacggt gcgcggtagt tttgccggca cggcgcaggt gttccaggag
    23581 acgatgaact cgcaggtgat cctgattatc gccgccatcg ccacggtgta tatcgtgctg
    23641 ggtatccttt acgagagtta cgtacatccg ctgacgattc tctccaccct gccctcggcg
    23701 ggcgttggag cgctgctggc gctggagctg ttcaatgccc cgttcagcct aatcgccctg
    23761 atagggatca tgctattaat cggcatcgtg aagaaaaacg ccattatgat ggtcgatttt
    23821 gcgcttgaag cccaacggca cggtaacctc acgccgcagg aagctatttt ccaggcctgt
    23881 ctgctgcgtt ttcgcccgat tatgatgact accctggcgg cgctgtttgg cgcgctgccg
    23941 ctggtattgt cgggcggtga cggttcggag ctgcggcaac ccctggggat caccattgtc
    24001 ggcggtctgg tcatgagcca gcttctgacg ctgtatacca cgccggtggt gtatctcttt
    24061 ttcgaccgtc tgcgactgcg tttttcgcgt aaacctaaac aagcggtaac cgagtaaatg
    24121 acagatcttc ccgacagcac ccgctggcaa ttgtggattg tggctttcgg cttctttatg
    24181 cagtcgctgg acaccaccat cgttaacacc gcgcttccct caatggcgca aagcctcggg
    24241 gaaagtccgt tgcatatgca catggtcatt gtctcttatg tactgaccgt ggcggtgatg
    24301 ctgcccgcca gcggctggct ggcggacaaa gtcggcgtgc gcaatatttt ctttaccgcc
    24361 atcgtgctgt ttactctcgg ttcactgttt tgcgcgcttt ccggcacgct gaacgaactg
    24421 ttgctggcac gcgcgttaca gggcgttggc ggcgcgatga tggtgccggt cggcaggttg
    24481 acggtgatga aaatcgtacc gcgcgagcaa tatatggcgg cgatgacctt tgtcacgctg
    24541 cccggtcaga tcgggccgct gcttggcccg gcgctcggcg gtctgctggt ggagtacgca
    24601 tcgtggcact ggatcttttt gatcaacatt ccggtgggga ttattggcgc gatcaccaca
    24661 ctgatgttaa tgccgaacta caccatgcag acgcggtgct ttgatctctc cggcttttta
    24721 ttgctggcgg ttggcatggc ggtattaacc ctggcgctgg acggcagtaa aggtacaggt
    24781 ttatcgccgc tggcgattgc tggcctggtc gcagttggcg tggtggcact ggtgctttat
    24841 ctgctgcacg ccagaaataa caactgcgcc ctgttcagtc tgaaactgtt ccgtactcgt
    24901 accttttcgc tgggtctggc ggggagtttt gccggacgta tcggtagcgg catgttgccc
    24961 tttatgacgc cggttttcct acaaattggc cttggcttct caccgtttca tgccggactg
    25021 atgatgatcc cgatggtgct cggcagcatg gggatgaagc gaattgtggt acaggtagtg
    25081 aatcgctttg gttatcgtcg ggtactggtg gcgaccacgc tgggtctgtc gctggtcacc
    25141 ctgttgttta tgactactgc tctgctgggc tggtactacg ttttgccgtt cgtcctgttt
    25201 ttacaaggga tggtcaactc gacccgtttc tcctccatga acaccctgac gctgaaagat
    25261 ctcccggaca atctggcgag cagcggcaac agtctgctgt cgatgattat gcaattgtcg
    25321 atgagtatcg gcgtcactat cgccgggctg ttgctggggc tttttggttc acagcatgtc
    25381 agcgtcgaca gcggcaccac acaaaccgtc tttatgtaca cctggcttag catggcgttt
    25441 atcatcgccc ttccggcgtt tgtctttgcc agagtgccga gcgatacgca tcaaaatgta
    25501 gctatttcgc ggcgaaaaag gagcgcgcaa tgaagttctg gcgacccggt attaccggca
    25561 aactgtttct ggcgattttc gccacctgca ttgtcttatt gatcacgatg cactgggcgg
    25621 tacgtatcag ttttgagcgc ggctttatcg attacatcaa gcatggtaat gaacaacggc
    25681 tgcaaatgct cggcgatgcg cttggtgagc agtacgccca gcacgggaac tggcgcttcc
    25741 tgcgtaataa cgatcgcttt gtatttcaga tcctacgttc actggagcat gataacaacg
    25801 aagataagcc cggccccggt atgccaccac acggctggcg cacgcaattt tgggtggttg
    25861 atcaaaataa taaagtgctg gttggcccgc gagcaccggt tccacccgac ggcacacggc
    25921 ggcccattat ggtcaatggt gcggaagttg gcgcggtgat cgcctcccct gttgaacgac
    25981 tgacccgtaa tactgatatc aattttgaca gacaacagcg gcaaaccagt tggctgattg
    26041 ttgctttatc taccttatta gcggcgctgg cgacattccc actggcgcgc ggtttgctgg
    26101 ctccggtaaa acgactggtg gatggcacgc acaaactggc agcgggtgat ttcactactc
    26161 gcgtagcccc caccagcgaa gatgagttgg gcagactggc agaggatttc aatcaactcg
    26221 ccagcacact ggagaaaaac cagcagatgc gccgcgattt tatggccgat atttctcatg
    26281 aactgcgcac gcctttagca gtactgcgcg gcgaactgga agccattcag gatggtgtgc
    26341 gtaaattcac gccggagacg ctggcttctt tacaggcgga ggtcggtaca ctgaccaaac
    26401 tggttgacga tcttcatcag ttgtcgatgt ctgatgaagg cgctctcgcc tatcaaaaag
    26461 caccggtaga tttgatccca ctgctggaag tggcgggcgg cgcatttcgc gaacgatttg
    26521 ccagccgtgg cctgaaactg caattttccc tgccagacag tattaccgta tttggcgatc
    26581 gcgatcgttt aatgcagttg ttcaataact tactggaaaa cagcctgcgc tacactgaca
    26641 gcggcggcag cctgaaaatc tctgccgagc agcacgacaa aacggtgcgc ctgacctttg
    26701 ccgacagcgc gccgggcgtc agtgacgatc agctacaaaa attgtttgaa cgtttttatc
    26761 gcaccgaagg ttcccgcaac cgtgccagcg gcggttccgg gctgggactc gcgatttgcc
    26821 tgaacattgt tgaagcacat aatggccgca ttattgctgc ccattcgcct tttggcgggg
    26881 taagcattac agtagagtta ccgctggaac gggatttaca gagagaagta tgaccgagtt
    26941 accaatcgac gaaaacacac cgcgtatttt gatcgtggaa gatgaaccga agctgggaca
    27001 gttgctcatt gattatctgc gtgccgcgag ctatgcgccg acgcttatca gccacggcga
    27061 tcaggtactg ccgtatgtgc gccagacacc accggatctg atcctgttag atctgatgct
    27121 ccctggcacc gatggcctga cgctgtgccg ggaaattcgt cgtttttctg acattccgat
    27181 cgtgatggtg acggcaaaaa tcgaagagat cgaccgcctg ctggggctgg agattggcgc
    27241 cgatgactac atctgcaagc cgtacagccc acgggaagtg gtagcgcgtg tcaaaaccat
    27301 tttgcgccgt tgtaaaccgc aacgcgaact tcagcaacag gatgccgaaa gcccgttgat
    27361 gatcgacgaa ggtcgttttc aggcttcatg gcgcggtaaa atgcttgacc tgacgcctgc
    27421 ggaatttcgt ctgttgaaaa cgctctctca tgaaccagga aaagtgttct cccgcgagca
    27481 attgctcaat catctttatg acgactaccg cgtagtaact gaccgcacca tcgacagcca
    27541 cattaaaaac ctgcgtcgca agctggaagc tctggacgaa gagcagtctt ttatccgcgc
    27601 cgtctacggt gtcggttatc gctgggaagc cgacgcttgc cgcatcgttt agttttagcg
    27661 acatgacttt gttagccgga gatggcgttc agatcagatc cggcaacatt atcccacgta
    27721 tggtcagcaa actgacatta tctctccgcc ctggcaggca ccagaaaatc gctcatactt
    27781 taatcggtaa acagcacctt tagatgctgt tttcgatacg caatttcaat caaggagtca
    27841 ttatggctgg ttggtttgaa ctcagtaaga gcagtgataa tcaatttcgg tttgtgctaa
    27901 aagcgggcaa tggtgagact atcctcacca gcgagctgta tacctcaaaa gcctctgcgg
    27961 aaaagggcat cgcgtcggtg cgtagcaaca gcccgcaaga agaacgctat gagaaaaaaa
    28021 cggcaagcaa cggcaaattc tatttcaatc tgaaagccgc caatcatcaa attatcggct
    28081 ccagccagat gtacgccacc gcgcaatctc gtgaaaccgg aattgcctcc gttaaagcca
    28141 atggcacaag ccagacggtg aaagacaata cgtaattacg atgccgggcg cgtgttgttg
    28201 gcgtccggcg ctttaccagc tcccccctac agcgctacaa tgcccgccct taaagtgggg
    28261 gcactcccct ggccgcttca tcaggtgaag cggatctgac ctgtcatcag aacgagagaa
    28321 ttatgtttaa accggaactc ctttccccgg cgggaacgct gaaaaatatg cgttacgctt
    28381 tcgcttatgg cgcagatgct gtttatgcgg gccagccgcg ttactccctg cgtgtgcgca
    28441 acaacgaatt caaccacgaa aatcttcagc tcggcatcaa tgaagcccac gcgctgggga
    28501 aaaagtttta tgtcgtggtc aacattgcac cgcacaacgc caagctgaaa acctttatcc
    28561 gtgacctgaa accggtggtg gaaatggggc cggatgcgct gattatgtcc gatccagggc
    28621 tgattatgct ggtgcgtgag cacttccctg aaatgccgat ccatctctcg gtacaggcta
    28681 acgccgtgaa ctgggcgacg gtgaaattct ggcagcaaat gggcctgacc cgcgtgatcc
    28741 tctctcgcga gctgtcgctg gaagagattg aagagatccg caatcaggtg ccggatatgg
    28801 agatcgagat cttcgttcac ggtgcgctgt gcatggccta ctccggtcgc tgcctgctct
    28861 ctggctatat caacaagcgc gacccgaacc agggtacctg caccaacgcc tgccgctggg
    28921 agtacaacgt ccaggaaggg aaagaagatg acgttggcaa catcgtacac aagtacgagc
    28981 caattccggt gcaaaatgtt gagccgacgc tgggtatcgg cgcgccaacc gacaaagtgt
    29041 ttatgatcga agaagcccag cgtccgggcg agtatatgac cacgtttgaa gatgagcacg
    29101 gcacttacat catgaactcg aaagatctgc gcgccatcgc ccatgttgaa cgcctgacca
    29161 aaatgggcgt gcattcgctg aaaatcgaag gccgtaccaa atctttctac tattgcgcac
    29221 gcaccgcgca ggtttaccgt aaagctatcg atgacgccgc tgcgggcaaa ccgttcgata
    29281 ccagcctgct ggaaacgctg gaaggtctgg cgcatcgtgg ctataccgaa ggtttcctgc
    29341 gtcgtcatac ccacgacgat tatcagaact acgaatacgg ttattcggtt tctgaccgcc
    29401 agcagtttgt tggtgagttt accggtgagc gcaaggggga gctcgcggcg gtagcggtga
    29461 aaaataaatt ctccgttggc gacagccttg agctaatgac gccgcaaggc aacattaact
    29521 ttacccttga gcatatggaa aacgccaaag gtgaagcaat gccggtcgca ccaggcgatg
    29581 gttatactgt gtggctcccg gtgccgcagg atctcgaact gaattacgcg ctgctgatgc
    29641 gtaatttctc cggtgaaacc acgcgtaacc cccacggtaa gtgattaatt tcgattattt
    29701 ttcccggatg aaaaattctt agaaaccgat cacatacagc tgcatttatt aaggttatca
    29761 tccgtttcgc tgaaaaacat aacccataaa atgctagctg taccaggaac cacctcctta
    29821 gcctgtgtaa tctcccttac acgggcttat tttttacgcg taatacaatg aaataaaagg
    29881 atttatttct ggtcacgtcc acacattgac cacatcgaca aaaaagcccc tcgactgagg
    29941 ggcttcctgt ttgtaattac atccacataa tttgctgccc tgacggcaac gggtgcggtc
    30001 ttacggcgtg gacttctccc ggcttcacga tgtatcgctg taccgactca taagtgatga
    30061 acgtggcgct gcaattcacg ttctggcact ggtgataacg ctcttttgtc gtgtcagtga
    30121 tatagcgact tgtacgcgca tgtgcggcat gctggcataa aggacaatga aacatcgcga
    30181 gcacctcttc cggttttgtt gatggtgcca ttttagttaa tttatcctta taaaacaaac
    30241 agataaaaca aaaacatcac tcatcatctt ctgtttcgta ctccacatca gaaagcctga
    30301 cctcaagctc caaggacgtc gtgaagccgc tattattcag aaaatgtgtc accttagtga
    30361 ttgtccagtc ctgctcgtct atgacgcgct taaagcctga cactttgacc ggcgtttccg
    30421 tgtaaatatc tgcccgacca gtagccagac tgatggagaa ctccgcaacg ccccgttgca
    30481 gcttatccca cttcgcctga gcggcgcgca tggcctgtgc tttcgtggca tataccgtgg
    30541 tcagggcaaa aacgttgtca gcctcaccag ccatgtattc accttcgcgc gcttccggta
    30601 cttttggcgc tttcttctgc gtgactggtt tcgctttcgg gtgctccagt gcgcgcaggt
    30661 gtttctcttt ctttttgcgt ttcagtttta ccttctgctt ttgtggcttc gggtctttgg
    30721 tgtgtaacca ctttgccgtt acgccggtat aggctccacg gtcagcaatc gcaaaatgat
    30781 ggcggtcgcc gtcgctgcgg gttatggtga cctgcgggat ttttttaccg ctggccgtca
    30841 ccccctgccc cgctttgaga aacaacagtt ttcccatttt taccgacacc tcaccgccgt
    30901 tgcgttctgc aagacgggtc aggaatttcg catcagactc ctgcgactgg tcgatgtgcg
    30961 ggattttaat tccggccagt gacggagcga cactggcttc cagcctgtta cgggaggcta
    31021 tcgcctcaac aatcgcaccg agcgtggtgt catgccagga gccttcacgg cgggaattga
    31081 gcgtcccgcg aaaatctgca ctccgggcgc ggatggtgac cacatccggt gcgccccggt
    31141 gttcaacctc atcaacggta aatttccctt tgcataccag ggcaaaacct ttccagccga
    31201 tatacaccgt caggacagcg ccacgaaccg gcagcccgac ctgcccgtcg gcatcgttca
    31261 gttcaatatc aagctggtca gcctcaaagc cccggttatc cgtcagggtc atgctcatca
    31321 gtcggtcgct gatattgccg gaaatatccc tgctgtcgag catcagcatg taatccggcg
    31381 tcagcgtact gcctgcatca aatgtcagtg catccagcat tatcccgccc ccgtcatacc
    31441 cgtgaatctg gtcgccatac tgccagcctt accgatgagc gattccgcct gtttaccgat
    31501 atcgccataa agcgcggcca gtgattcatc aacgcgggtg agcgacagcg taaaatcaat
    31561 ttttcggggt gtgccgtctg caaagaaaat acttcctgtt tcactcaccc tgctgatgac
    31621 atacatgccg taaatcatgc cggtgccatc cagcaacggc cacgcccggc cttcctctgc
    31681 catcagcctg agcgtggtca tcgtcagctt gccgccggtc agttctggat aaagcacacc
    31741 ggcaagcgtg atgttttcct cacccacacc gagaaactgg taggcgtccc gtttaccgat
    31801 acgggaattt gacggccagc gataatctga ttcacgctgc atggtctggt gtggcagcgt
    31861 ctggcgcata aaaacaaaca tacctaacgc gagcatcatt tttcgtcacc tccttaaccg
    31921 tcatgcatca tgctggcacg ggcgcgcgca cgtttatccc gctcgtattt ttcgagcgca
    31981 tcctgtaact ggcggtcaag ctgtgtcccc ggcgcagtac cacccgtcag gctgatgtga
    32041 tattcgtttt tactctggtc cacataagag cggccagccg gtgccgtgac cggctgataa
    32101 gcctgatagc ctgcataaga gctggtcgcc ggaatataac caccggtgcc atacgtggcg
    32161 gcatgagttc tggcggcggt ctggtcaagt gtgtctgact ctttgttgat aacaccgagt
    32221 ttttccagta cccagtcaat accgctgcgc agtttgttga acgcattaag cggcagcatc
    32281 agcgcgtcag ccagtgcctg cccgaacatg acgcccgtgt cacggcaacg gttcagggtg
    32341 tcctgtgtgg ctttaaccgg agcaatcagg tttgtaaacc actgccacgc ggcctgtaac
    32401 ttttcgccca gccagtcaaa caccggctta agtggcgtga acagctcccc caccggcgca
    32461 aatgccgctt tcagcccttc aaccacaccg ccaaagaatg cgctgacagg ctcccagtat
    32521 ttacggataa gcaacgcccc ggcgacaatg gcagccacca cggccacaac cggccagcta
    32581 atcgcaccga tggccgtcat aacggcacta ccaaccgtcg tgaagattgc ccccattgcg
    32641 cctgctgccg cgataatggc attgatgccg gtgataaccg gccaggctac aaggccaatg
    32701 gcaccgatga tgccagtcag caccagtgca ccgccggcaa tgatgccgat ggttgacgcc
    32761 agtgatttgt ttttctgtat ccagccgtcg agttttaaca catactttgt ggccgtctgc
    32821 gtgagcttac gcagtgcgcc ttcctgctgg tcaaacaggt cagtccccac cgcctcataa
    32881 gcggactgaa actccttaaa gtcaccgccg agattgtcct gcataatatt taccagctct
    32941 gaggtcttcc cgtccgaggc tttaaacgca gcggtcagtt tgtccagctt tccggttgag
    33001 gcgacagtca tcagcacggc ggcggctgag ctggcctcct ccccgaaaat ggttttcatg
    33061 tattcagcct gctgggcagt accgagccgg tttttctcaa aactggcctg catttctttc
    33121 agaatggtaa atactggtcg ggtgtttccc ttgctgtctg aggttttcac tccaagctct
    33181 ttgagtgcat cccatgcttt tcccgtcggt gcctgcaggc ggcttaacac ggcacggctt
    33241 cccgtccccg ccattgagcc tgtgattttt gcatcatgca gcgccccgac cattgcggcg
    33301 gtttcttcaa tgctgacacc ggcatttttt gccacaggtg cggcataggt cagcgcatcg
    33361 ctcatgccgt caaaatcggc ggcggttttg ttcatcgtca tggagagaac atccccgata
    33421 tgagcgacct tatcgtttga aagctgaaag gcggatttca tccccatcag cagggcggcg
    33481 ttttcttcca tcgtgcggcg gttcgccagc gccatattca gcgtgaccgg cgttgccgcc
    33541 tgaatggcat caacatcccc acccgctttc gcgatgatta tctgtgcacc ggccgcatca
    33601 tccgccgagg cggcggtatt gtcgccgagc tggcgcgcct gtttgcgtag tgcggtcatt
    33661 tcggcggagt cttttgccac acccagcacg gcctgcaatt ctgagttttt ctgcgcaaac
    33721 tcataaccgg acatcagcaa cttaactccg gccatcgttt ccgccgccgc aatccccaca
    33781 ccggcagcgc ccactgaggc catatttccg gccagttcct ttccggcctg ataacgctgt
    33841 tttactgcat taagttttgc ctgttgcgca ctgacacgcg ccagcgcgtc acgctgccgg
    33901 ttaagctgtg cggtggtttc actgatacgg tttttcagtc cctgctcatc atgtgcaaga
    33961 ttgcgggtat taattcccac agcggccagt tcccgctgct ggcgcttaac ggaatccgtc
    34021 aggcggttat atttcgcctg taagtcctcc gccgcacgct ttgcggattc cagcactttc
    34081 gcctgagcac gggtcggacg ttcagtgttt ttaaactgtg tggcaagtgc ttcggcttcc
    34141 tgccgtgcct tttcaagtgc atgaccagtc acggcaagct gtgcgctggt cttgcgaaat
    34201 ccctcaatac gggatgcgtg accgttcagc tcgcgcagtg atttttgtgt ttcccggata
    34261 tcccccgaca gcgacttact cgctgtacgg atggatttaa acgggcggga tgcctggtca
    34321 acagccctga gcaatacctg taattttaca ttgttactca ttcgtgcttc cgcttcgccg
    34381 gagcgccttt tcgcgccatg tgatgagttc ggtcaggctc atgggataca gttctgatgg
    34441 cggccagtga aatatcactg ccacatctgc catcaggtca tcgaccgaca gatttttcgg
    34501 aaacgtcact gcaccgagtt cggagacaaa aaaccgacca ccttaccggc cagcgccaca
    34561 aggtcaggca gttccagcgc ggcgacctcc tgctcggtca gcatcggtgc cgtcatgcgc
    34621 ggcagcacct taatcagtgc atcgacttcg gagtttgcga ccgcagccag actgacaccg
    34681 cgcagcgtcc cggcattagg tttcatcagc gtgacctgtt cgataacctg ctcaccacgc
    34741 ttgaccggat tgtccagggt aatcacattt tctttgttca tggttttctc acttctgaat
    34801 cggggttaac cggtcagcca ggctgaccgg atgaaaatca caggccgata ttgcggcggt
    34861 gttgctccag ccggtcgacg ccgttcacct tctcaatcat gttgatggtg tcgatttcga
    34921 ccagctcctt accgtccatc gtcaacttga aataagtaca aatcaccggg agtttcgatt
    34981 cggtatcctc tccctgttta ccctctccgg tatcgacttc tttctgacgc ccacgcatga
    35041 ccacttcgac ggccaccgtt tcgccggtat cgtcgcgctg gtaagaacca gcaaaacgaa
    35101 tcggtacggc atcagcactg gttgcagcgt aaagctccca gataaccgaa tccgggaatc
    35161 caccgagcga ccactccatt gacagagcat catcatcaag gccgaggtct accggtgcgc
    35221 tgccgttcat ccccgcaccg cgatagtttt cgagcttacg ggtcagtttt ggcagcgtga
    35281 cggactttgc aacgccctga tagctgtagc cgttcagaaa gacgttcatt aacttgagtt
    35341 tgcgcggcat tgccatcggt caggctcctt aattgctgtt aaccgaagtg accagacttg
    35401 ccaggtatct ggaagtaata cgctggcgca gggtcaggtt ttcgagagga ggcaccgggg
    35461 tatagtcgta gtcgatatac agttttccgg ccttgagggt ttccgcatcg ttggattctt
    35521 cgctgaacca gcatgtgcca tccacgatat aaccgttgtt tttcagttca cggaatttgg
    35581 cattgatgcc gtcaatgatg tcgcgaatca gcgttgcggt gatgggctta tcaatagccc
    35641 acatgtgcgc ctcagccatc gtgtcggcca gtacctgcgc ggtgcgtgtg tagctttcaa
    35701 agaggaacag cgggtcatca gagcaggtac ggttacccca gaaacggaaa ccgtcgcggc
    35761 gaaccagcgt tgtgacgcct gcctcgttca gcaggtcagc atcagtgcct ggcttctgca
    35821 aatcccagaa gacggatgca ctgatgccgg taacaccgtt caccccgacg ttggacagcg
    35881 ttttatgcca gccctgctcc tggtcgattt tagcgcgcag acccagcgca cgagcggtgg
    35941 catacgcggt ggcggtggta ctggtaaccg tatcccatgc gaggaaatcc ggccagataa
    36001 ccatcagctc acgctggctg aaattctggc ggtaggcttt cacctcggaa atggttttac
    36061 aaccccatgc gctgatatac ccgaaggcat tcagttcctg gcataccgat gccagtgcga
    36121 cggcgacctc tttggtgtcc agtcccggta cgccgagaat gcgcggttta acgccggtaa
    36181 ccgactcagc ccccatcagg gctttcagtc cggtgtactg accgttttcg tcggtggtgc
    36241 cgataatatt ggaaacagtc tgcgcgagtt tcgtttcctc gtcgtcgccg gtgccgtctt
    36301 ccacgcgcac aacaacggtg accggttttg actggtcagc gatggcctgt aacgacgccg
    36361 ccagcgtgcc ttttttaccg gcctttgcaa ttgcgctctg cacattggta atcagcacag
    36421 gtttattgag ggggaatgtt tccgcatccg catcgctggc cgtgcagacc atgccgacaa
    36481 tggcagtgga tacggtggaa atgacgcggg tgccgtcgtt aatctccagc acctgcacgc
    36541 cgtgatgata gtcactcatc cgtctaactc cgtggttaat gggtgagtgg tattttctgt
    36601 tgtgcagagc atgagacgct atttgacctg gctggtcagt ggatgaaaca acagataaag
    36661 aaaaggcgga caatccgccc gcctgtcatg atttgtactc acttatttcc cgactgacaa
    36721 tttacatagc caaaacgcta tcaaatctga cagtctgctt tgagcgagaa gcggaagttc
    36781 aatgtgataa agctcaggaa cattgtcgtt gatgatgata aagaggatca gactaactta
    36841 gcacttctca tcacgttgat aaggcataga aaccgcaaac actattgtga atataatttc
    36901 actatagtaa attaaagtaa gctcttttcg ttcgttatta cataaaataa agttctctct
    36961 aaggttgaaa aatgttagat atcagaatgt cacgaccatc tgaagcatca gagatcatac
    37021 aaatctggaa gagttcagtg gatgctaccc acgactttct tacaggtcat gaccgacaag
    37081 aaatcgaaaa agaagttatc ggctttttct cagaaactcc tgtatgggtt gcaacaaatc
    37141 aggatgacca accgctagga tttatgtttt tgcatgaggg gcatctggag gcactttttg
    37201 tagcaggctc tgctcgcgga cttggcgtcg gaaaacgttt gatatctcat gcactggcgc
    37261 tgcatccaga gctaagcgtt gatgtaaatg aacaaaacca gcaggctgta gggttttatc
    37321 agcatatggg ttttcaggta tcagggcgtt ccgatctgga taatcaggga aggccatacc
    37381 ccttgttgca tctgagaatg gccaataaca tgtaaccaat gtggcagaga agatatcatt
    37441 gatttttttg gctaaaagcc acgatgtaag aacagctctg ctcactatct ggattgctca
    37501 tttggtactc gcttgcaaag gtaagggcaa gacagtcacc ggcacgtaaa tgatgggtct
    37561 gatttttgac tcgaaaatca aggctgccac tcatcatcca aagcgtttgc ccggaaagat
    37621 gctcatttgc ggaggcgggt atcgtcagct cacccattgc agggatttca actttgatca
    37681 gttccgggca tgcccccgca ggagacaaag accaacgggt gatgcctgat tgctcatccg
    37741 tccagtgctg ttgctgatga gcaaaacgaa ccagggaatt ctgatgttgc tcaagctctg
    37801 caaagagttt agatagcgtt acattcatgg cgttggccag gcggctaagg atagttgcac
    37861 tagggctgga gatgcctcgt tcaatcttac taatcatggc ctgactgacg cccgatcgtt
    37921 gagcaagctc tgttacagtc aaattgcgag ccttcctgtg tttaagcaat aaacgtgcaa
    37981 tatcgctatc tactatctga tttttaaatt ttttatccat ttccgccctc tctttcacca
    38041 acctgattat gccattacat aaattggcct gaaataaatg attttgtaag tctgtaaatt
    38101 cactactcct aagcgcggca cttcgtttat acgcacctat aaccttaaaa tatgagttgg
    38161 taagcttaac gtccgctttt ggcacatagc ggactgtcag attaggcttt actctgtgct
    38221 atagatatgt aagctcacac cagagatcat acaacttatt gcggcatttc cggccattcg
    38281 ggatttgcag gatccacacg actgaccaga acgctgtagc gttcccatgc ttccagtcgg
    38341 ctgcgttcct catccgtcgc catattcagc ctgacagcgc gttccagcgg caaaatcacg
    38401 gattcagctt cggaaagtaa agctgccttt tgtgattctg cctgttgctc ctgttcatct
    38461 gccgtataaa tccgcttaat cacggcacca tccttaaaca tccatttacc tgagtcatca
    38521 gcacgtcggt tggaggtaat atcaggaacc tcgacaacgc tgaaaccttc agggttaagc
    38581 gttgaagcat ctctggtgat gccgacaatt atattattct cgtcgtaaac aatctttatc
    38641 gtgtcttcct gaaaattact tacttcctca taccagtttt ttccctcttc ggaccataac
    38701 cagataacat caaaattttt tgtcagttga tattgggcaa cagtttttgg attacccgca
    38761 gtaatatttt ttaaatgctg cataaattac acctgtgcga cgttatacca tgtgccattg
    38821 atgtattttt gtattggcct gaatactgcg gggtcatcac catcgacttc accgacaata
    38881 ccaagcccgg taattacgtg ccctgctttc tcatacatca cccctttctg catggtctgg
    38941 acaacacgtg tgccaagtct gatatctctc acatagcggg aatcaaagtt accgtaatcc
    39001 gagggattaa cacgccccgt aatatttatg gtcttattac tttgaatgct tccggaaaca
    39061 aagcgcataa catggacgtt attagcataa acatccagat taccatcgcc attttgttta
    39121 aatccggtgt cgttatcacc aagaacaata gagttcccac ccaacgcgtt attcgtgcca
    39181 agtgccagcc cgccatcaat cctggcacca ttaccaacag acacaactcc tgttcttaag
    39241 ttgatgccga atggccttaa tgacccaata tctccatttt caccctcatt ttctcgtgta
    39301 ggaatgatat acaggttttc ttcagaacgg cgaaaaatag caccaaaaga tgaattaaat
    39361 atcctcagcg cattgactgt agatattttt acttcactac tgaaaagggc tttaacaaga
    39421 acatacagag catcccattt aagattcatc aggtcttttg ttgtggtgct ctggcgactt
    39481 ctccatttga aatattcatt gccgttgtca cctgtttcaa accacatata tgaatcagta
    39541 tcgctgtcgg catcattttt aaatccaatc tttgcccagt cagtatttcg aatccaggca
    39601 aggattgagt cgttttcaaa agtaagcccg ccggacaagg tatcgccatt tttttgcacg
    39661 gcgttcccgg ctcggtttac cgtttcctgt aaaccgagat attcgataac ggcggcaacg
    39721 gtcgatttag ccagaatatc ccgcccgact tttgtcaggg tcgccaggct ggcaacatca
    39781 ttccccgtaa aatacggaaa cctgtctgcc gcagtagcaa gccccgccag cgccgtcaaa
    39841 gtggcatctt tcggttgctt acccgcaagc gcattagtca tggtggtagc aaaattcggg
    39901 tcattgccca gcgccgccgc cagctcgttc agcgtattca gtgcgtcagg cgacgagtct
    39961 acaagggcgg caatcgcggc cataacgaaa gccgtgtttg cgatctgagt attattcgtt
    40021 ccctgtcgcg cagttggcgt cgttggcgtt ccggtcagtg cagggctgtt taatggcgct
    40081 ttcttgttcg tttcatccat taccgtctta acggcttttg gtgttgcggc gagcgtttca
    40141 gacatgctgt tggtcgcact acttagctgg acaagacctt ttcgcgctgt ggtagcgtcc
    40201 tgtgcggtat atttcccgtt agcaaggtca tacgcggcct ttaccgcttt cggcgttgcg
    40261 gccagtgttt cagacgtgct gttggtcgca ctgcttaact gagtaaaacc tttggcggtc
    40321 agcgaggcgt ccgggtgacg tcgtgactgt tcgtgctctg caattttgtc atcaacgtaa
    40381 tcctgtgtcg ccatcaccgt tgtggtgtca atggtcagct ccaccgaggc cacactgctg
    40441 acgatgatga ccatgcgaca ggtctgcgaa cgccctgagc cttcggcaag agctggctta
    40501 taactttcgg ccatgttcgc cacggcaatt aacgttcccg catcatcgta caggccaagc
    40561 tcacgcatcc agaaaccgcc cacctccggc ggaataacca gctctgcgat aatataatta
    40621 ctgtttcgtt tgtcctggct gattttgttc agcgtatgtc gccagacttc gtggataagc
    40681 ccggtctgtc cggcatccgg gacaggcaat ttaccaccgc catccccgac ggccatcgtg
    40741 gtaatgttga ccttccgccc tcccggtgcg gttgccgctg ccagctttgc tgcaccggca
    40801 gtggtgataa cggttttgaa ttttgtgctc attattcctc acttatccgg ggtaaaccgt
    40861 aattacatcg ccgtcgtaag ccacaccacc ggcgaacagg tagccgggaa tgtcccgggt
    40921 aatgttcagg ccaataaggt ggcggcttgc aggtttggca tcagcaatca gccgttccat
    40981 ttcctgatac attgcctctg tgatgccact ttccagtaca ccaatatcaa gccggaaggt
    41041 gcccggcggg tcactggttt cccaccactc cgtcacgttg atgagatagc ccagcggctc
    41101 caccacacgc cggattgcac ctatagtgcc tttatgacag tggatgaaat aggcatcgcg
    41161 gataacagcg cgttttgtcg cttccggcca cttttcatcc catctgtcga ccgaaaacgc
    41221 ccacgccagc cacggcagca gatttgccgg acaggagtcc gggttccaca gctcacgaat
    41281 actgaccggt gttttttcaa tttccgcaca ggcttttgcg gcggcgacct caagcggtga
    41341 tgagccggtc ggcaacagtc gcgaatcact catccgagcc tccggtcacg acgcggtatt
    41401 cggtacagaa agacgcctgc gtactgttga gcacgatgtc ggccagtggt gcggccagct
    41461 cgacacgctg cacgccttcc acatgcaaag cggcataaat ggcagacaga cggatgtcgc
    41521 gccccagccg gtgctgtgcc gtgatgtacg cttccagttt tttcacggcg gcagcgcgta
    41581 tgggttcgct ttcgggacca gggtaaaggt aaagcgtggc gtttatctgg tattcaacga
    41641 tggcggcaga ctgcacggtc acgcggtcgg ccaccggcct gacgtcctcg ccattaaggg
    41701 cgttacgcac caccgccagc aggtcttcgg atgcgacacc gttattttca cgtgacagca
    41761 cagagatggt gacgcaggcc ggagacggac tggtgacaga gatatccgcg acacgcccgt
    41821 cagcactgcg accatgatac tgataggcac ccaccgaccc ggcgacgctt aaaccttcaa
    41881 aggcctgctg aatacgcaga cgataatcgg tgtcagattc catcacggcc ggtgtcggcg
    41941 ggatggtcga atcatctgcc ggggtgatag tcagacgcgt ggtgttgtaa ttggcaccaa
    42001 tcacatcaag gtcattaccc gcggcacagg ccagcatcac cgcccgtgcg gcctcattca
    42061 cacgctgacg ccagagaagc tcacgataag cattttcctc cagcagtttg acgagaggct
    42121 cagattccag cgtcagggta cgggcgaccg cctcctgctg gtcttccggg taaagggaaa
    42181 tcagtgtcgc cttgcgttcg gcgagaatgg tttcaaagtc cagctcctcg accacatccg
    42241 gtgcgggtag ctggttcagg tcgataatcg gcatggtttc aactcacagg gatggttaac
    42301 gaaagtggct ggccggtgtc gttgtgctgg ccggttaacg tgaccgtcat tcgcccgtca
    42361 aaactgcgcg ccgtggtgac agatgacagc gtgacgcggg gttcccattt cagcaccgcc
    42421 atgtaacagg cgaccttaat ctgcaactca agcgccgggg tctgcggctg gtcaatcatt
    42481 gatgccagca acgagccgta atcacgacgc atcacccgtg agccgaccgg tgtacgcagg
    42541 atatcgccga tactctggct gatatgctca aggtcagtga cagtcaggcc attactgcga
    42601 ttcattccga gataacgagc tgtcataaag gactcccggt tgtgccgccg ctgtcgccgg
    42661 ggtgtttgtg ggtatgcagt accttaccgt ttgatgagag ttcaccgccg gtgtgttcaa
    42721 tgttgccacg catcgtcccg cccttctgta cttccagcgt gctagtaatc agcctgttgg
    42781 tgcagaccac ctccggtgtg tccagggtga tgcgagttga cgctttcacc atgaccaccg
    42841 gcaccgtggc agtaacagaa tcagaagccg tcacgctggc cgttttaatt ccgcttaccg
    42901 tgagtgcact ggtttcgggt tcatactcaa tcaccgcccc gtcagggaaa cggatatgca
    42961 gggcatccgc cgacgcagac ggcgcggggt tatcgccgga ataaatcccc ggcagaacga
    43021 acgccgtgtc aagttcacca cccacggcca gaatcagcac ctgttccccc acggaaggtg
    43081 cccaccatgt gcgcgaacgc ccggcgcgat gggtcagcca ctgaagccag tcggtgcaca
    43141 tgccgccggt ctgcacacgg cagcgaccgg cgttaaggtc ggtttcgacg acaaggccgg
    43201 tacgaatcat gttgcgcagt gcgcgcgcga gttcctgaat atttgcgaga gtgttcatgc
    43261 gtgtgagatt gcacaatata taaaagttat gctatctgga ttcatttgta gaacgaccat
    43321 acaacattcg aggagagcgt aatgttcagt gataatgtga ttaatgcgtg gtggtttatc
    43381 tctttgtatc tatttttatt aatagcatta acatttgtta cctttggtaa aagtaatctt
    43441 atgaggttta ttgcacatca tttcaatctt gagtattcag acagaaagtt aaaaatgctc
    43501 gacaaaaaat ggcgcgacat tcaactattt aaaacaatta acggaatcaa tgtatcaggc
    43561 atcgaagatg tgagaatgat acagcagggg ctgattgatg gaaaactaaa aacatcgtat
    43621 tttttcctta ctcgcttctg gggtgacata acaaaaccac cacacataat taaaacaata
    43681 attgtaattc tggccagtat tatttatatt ctcttcgcat gttatataca caacaaacaa
    43741 tccgctatag taagagatgc cataggcata ccatataaaa atatgatgta ctatgtttat
    43801 agtgacaaag ttcttttatc cttcaaaaat aaaacagttg aacttaataa aacttatagc
    43861 cttgccgatt gcaagaccct gcaaaacgta tttataaaag acacacttcc tgaaatcgcc
    43921 tgcaataagc tcttacagct aaacgaggag gactccgaat ggttaagtca ggagattaaa
    43981 gataataaca gccacaaaaa agcattatta atactatccc tcgtctattt catttcaggc
    44041 ctgcttatat tcctgtcata tacaaaattc ctttacgcca ataagaaggt tttagaatac
    44101 aaagcatcaa ataaaaacca ctcataaacc tctaaacatt gagcgaccag cacggccgct
    44161 caatgtttaa ttgcgcatca gcctctgcct ggataaaact aacgctcaag gtgagccagg
    44221 atagtctctt caatcatctg cacatcctca ccggtaaagc cgagcagggg acgcgccgga
    44281 taatcaattt tcttaccgtc tttccggttt tcttccgaca ggccgaactg atgcacgctg
    44341 gcgatttttg gtgacttccc gccgtaaaat tccattgatg cctgttccgg gctggcgcgg
    44401 atatgcaaaa agcgactggt gataagtttc gcaaacattt ttcgcttaac acgaccggtc
    44461 ttttttctgg cgctctgctg ctggcgtggc gcgtagggtg tgccgtccgg ggctttctgt
    44521 gccatcaccc gacgctgctg actctgacgc agacgtttcg ccagttcggt actcagtcgt
    44581 cgacgccctg acggtgacag cgactcaatc agtccggtca gccggtcttc aaaacgctta
    44641 aactcattca tcccacttgc tcaccagttc gccattgata tacagctcca tcgggcgggt
    44701 aaccggctcc ggcggcggag gttccgggat attcttcaca tgcagcgcgc cgtcaacctc
    44761 actgaccagc gtgcgctcgg tcagcatcag gctgatgctg atatcaaagc tgctgtcatt
    44821 gttgatgtct gcataaaacg tgaagccctt tttctggcct tcgtcggtgg tcatgatgtc
    44881 gggctgattt tcccgcagcc acgccagcac cggcacaatg agcaggtcaa aatcaccggt
    44941 aaagtcggtc acaatcacat tgagcgtgta acgcttttcg aatgacatag acgtcgccag
    45001 tgtggaggca atactcccgt tatccacgaa tatccgcagc atctcgggac tggttttcag
    45061 caccgtgacg gcatcagtca gcgccctgcg caggctgtcg ggtttgagca tcgttttcgt
    45121 cctgacagtg tttaatcatt tttacctggc tggcacagcg tgccagcgcg ttctcaagca
    45181 gccggatatc ggcacttaaa tcgccgttct tctgcgggtc actgcccggc atcgggcaaa
    45241 gactcacttt cgggcaggcg ttgtggacaa tcactggcgt ctgcgcaggc cgggcgctgg
    45301 tgcaaccggc gcacagcatc aggcaggtca gcaccgtacc agcggcgaaa atcttcgttt
    45361 tcattgagta acctcgtgat ggttttctcg cgctgtgctt cacgcttcgc ggcgttctcc
    45421 agttcctgac gcagtgccac ctgcgccagc tcgtttttgt ctgccctggt gatggcaaca
    45481 tgaagctgat ttttcagcat ggtgatggtc gtctgctgtt cactggcgac gttattcgcc
    45541 ctgtccagcg aggcgcgcag gctggcattt ttgtgtttca ccagaaacag accggccacc
    45601 gccagcgata acaacacgac cagcacaatc atcagctttg acatagttcc cgcccctcaa
    45661 gacgctgacg acaggcttta cgtatcagcc ggaaaaacag cgacgccaca agataaatca
    45721 gcgcggtaaa aatccacccg gcagcgacca gcgagataaa cgtcgccacc atcaccacca
    45781 gagccgccgc ccgtctgcgc cacggcaccg gctgcaaaaa cagcgacgtg acaatcttca
    45841 cggccagcga ttccggcggc agctcccgcc cgtagagttc cagcacatac tccgtggcat
    45901 acacgccgac accaccggca accacacaga taaccgtcgc cagaatcgcc caggtggcga
    45961 caaaactgac ggccacgctc tgcgggtaaa tcagggacag tgccagcatc agcgccagcg
    46021 acacgttcag catcagtgaa agggataatt tcttcatggt gtttactccg tttaagccgg
    46081 tacgccgccg gcggtacgcc agacggtgac cagtttttcc agtgaatgct cacgctgacc
    46141 gtaaccggca cccggcaggg acgcccagat attgcgacag cgtgaaatgg cgcgctcaat
    46201 gcgtcccgcc cggatgtcat ccagtgcacc gcgttcgcgg atcaactgaa tggcgagcct
    46261 gtcctgtgac aacggactga aatccggcag ggcaagctgt ttgcggtagt gcggccagaa
    46321 caggtaaagc tgctgatagc gaccggaggc cgtggatttt tcaccgcgac ggttaaacac
    46381 cttcgccggt cggccatgcg cgaacgggtg atcactgtag tcggtgaaaa tttccggctt
    46441 tccgtccagt ccggtgacta tcacgtcata gccccggttt ttcgtcagcg gatgattcgc
    46501 cgtcccttcg gacacggcca gcatgtcgag aaaggcggcg atattctgat gcgtgttaat
    46561 taccggcatt acggtttccc cctgccctta aagcggcgct gaatggcaat ctcaatcacc
    46621 tgataaccgg cgatacccag catggaaccg atgccgcaca ccgcaggcag tgacaggtca
    46681 ggaaactgca ccagaacaac accggcaacc atcgagacaa aaccgccgag caacatgcgc
    46741 ccgataaaca gacgcggggt gatgggttca ccaccggcaa gcaccttgcc gacaacaatc
    46801 agcaccccaa tcatgaaaag cgacaggacg cttttttctt ctgctgtcat gcgttactcc
    46861 cacagattga cagtttcagc cacgggcgcg gtctgaacgt cgggcagttc gacggcggtg
    46921 ccgtgtggca gcaccgcacc cagttcagcc agtcccggat ttgcggcgag cacggtctca
    46981 accacgccct cagtgcgccc gtaataccgg acacaaatgg cgtcgagcgt gtcgccctgt
    47041 agcgcaaagg tcttcatcag atttgactca cgatgcagcg cggcttgtcc tggatacgcg
    47101 ccaccgccca gcgcatatcc cgccacagtt catcaatggt gctgtcaatg ctgtcggcct
    47161 tcttgtcgcc tttcgcactg gcatccacgc cgcgataacg ctcataaagc gacgcggtcg
    47221 ccatcgcaca cacggcgcgc tcgtagtaaa aaactttgat gctttcaccg tcgatgtcgt
    47281 ccgccggaac gtccgccaga cgcgtaaaac cggcggcaat tttctgttcg cggtactcgt
    47341 acagctccgc attcgtttca gccatgcctg acttgatggc ctcacgcaga cgggcggggg
    47401 cgacggtctg ctcaaggcgc atacgttccc ggacgcgctt cgggtcgata tcgggaaaaa
    47461 agaacgtgtt tttaatcacc ggctcgtcgc ctgccggttg cgggatgacc accgtaccct
    47521 caccggacac gggagcctcc tttcgcggaa taatcagcgt catcatgact acctctgaaa
    47581 agtcgggcgg tggacgccgg tgcagtgtca ggtaattcac cgtcactgac cggcgtgccg
    47641 ccctggcgcg gggcgcattc ggttgttaac tggctttctt tttcgggcgt ccacgttttg
    47701 ccggtgtcac gctccgggtc ttacgcggga cacgggtggc cgctttgggc tgcggctccg
    47761 gcttcggttt cagctcccgc tccagtcgtt caatctcttt tttgacgcct gcctgacagt
    47821 cgagctgtgt cgcacgttgc aggtgagcca gcgcaccggc ggcatcacca ccatcacgca
    47881 gaaacagacc ggtgattttg tgcagctttg cgcgcacttc atcaggcatg tcagccgtgg
    47941 cggtcagttc aagggtctcc gtcagcaggc ggatatccac agattcaccg gcagcgtgag
    48001 cgcgcatggc cgcgagtgcg acctcctcgg tgaacatgta cggcggggtg cggcggtgtt
    48061 tacccggcat ggtcagaccg tacttcaggg cataacgggc aatctccagc gcaccggcaa
    48121 tatcgccggt atccagacgc cacagcatga ccgtcatcag aatgtcatcc tgtgcacctt
    48181 tgccctgctc cagcacaccg ttcacccacg gcaaccagaa cggcagcagc tcgcgctttt
    48241 tcgcggcctt aagctctttt gaataaatcg ctttcagtgt gcgctggtct gcggccagct
    48301 taaccagcat ctgctcatag acagttgcat gtcgcagcgg ggcggcttcc cgctgcgcgg
    48361 tcatcgctgc cgagacccgc atcatgtggc gctgtgcggg actcgtcatc ggttacgctc
    48421 ccggctctgc ggtcgcctta gccggtgtgg agaaatcacc gaccttaatt ttttccacca
    48481 gacaaccggc ggcgtagtct tccactacgt aatcaatgtt cattgactcg tagttctcca
    48541 cgcggtcgag tttcgggttt tccacaatca cgcggcgatg gctgtcatcc atgtagtaga
    48601 tggacaggtt ttccagcttc gtgatgagca tcgcatccgc cgggaagtac gggacgcgta
    48661 ccgccggcag gttaccgatg cgtttctggc tgatgatgac gtcagcggcc agcatttcgc
    48721 tgttgtcctg ctccttgtta acgatgggga aatacttgtc cgccagtaac tgacgtccca
    48781 caatcaccac aaggtcaggg tcttcctgat accacggctc aatcaggtta ttggtcgcat
    48841 ccatcaccag tgcgtcaagg ctggcataat caccgccctt gcccacgcgg atgacctcag
    48901 aggtggtgtg cccttcctcg tcagtgacct tgctcatcac gcgcgccgga gcttcattgc
    48961 ggtatttctg cagccagccg actgccacat cctgcagcat cggattgctg ctgcggtcag
    49021 aggtttcggc acgcttcacg ccgttaaaac cggccatgat gaaatcaagg gactggcgtt
    49081 tgataatggc gttacggata cggagctgga aatcctgata acgcgcccac aggtccagcg
    49141 ttttgtagcg gatataaaaa tcgaagttaa tctggtcgca ttcgtacttg ttggacgcca
    49201 gcttcgagaa gtccttcggc tgacgctcgg tgccaccggc ggtgtcggtg gtgctggcaa
    49261 tggagccggt gacaccgatg ccaatttttt cccctttcat ttcgctgacc ggcacaatgt
    49321 tgatgcgggt cagaaagtca gaggactcct gcatggtgtt catcagggtc tgggtgaccg
    49381 acggttcaac ggtgaatttt ttcgacacat caccggcgtc gatgccgttc agttcggcaa
    49441 cacgggacag gtaggcatta aatttaaagc gggtttcctg gcgcatagtt tttcctgaaa
    49501 ttaagggtta atcgtgaagg ttttcccgga ctgactgacg ccggtcagca gttcgtcatc
    49561 agggcgtcac cgccaccacc ggtggccttg ctgcggcgct gctgggtcag actttcggtg
    49621 tggtcgagac tgtttttcag gcgggtgaat gcctggctgg tttcatccgc cctgtcagtc
    49681 acctcctgct taagtgcgga aaaggcggtt tccatctcag cgaggcgctg ctcagtggcg
    49741 ctcagttttt cctgcacatg ttcagcaaca gcggtcaccg cgtcatgcac gtcattcaga
    49801 cgggcatcat cgctggcctg tttgcggcca aaaatggact tcaccttttc ggtcagggcg
    49861 gtgaacacgg tttcaggcag gtcttcaaat tccagctcaa caggcgttgc cactgaaatc
    49921 aggttttcag ggcttaattt gaagcggttc agagggttgt gttttgccgt gcggcagaat
    49981 tccaggtatt ccgtgccgag gcttgccggg tcatcggtga cggccagccc caccagataa
    50041 catttgccgg tgttggcaaa gttcggctga atttccattg aggtatagac cttctgcgcg
    50101 gccttgttca tcgcgataag gtcatcggtc ggggtgattt tcgcaaacag cgcccatttg
    50161 cctttcagcg ccgaatcatc gtcaatcttt tcggccttca gttcgaccac atcaccataa
    50221 cgtttaaaaa taccgtcagg caggatgccg cgcagatgtt ccaggttaat acggcaacca
    50281 tagacacgcg ggtcaaaggt ttcggccatt tcctgaatat cctgcgcact gatgacacgc
    50341 ccgtcacagg tgtcaccctc aacgccgata cgaaagaatt ttgagacttt ttttgccatt
    50401 gtcaggagtc ctgaatagtg attagaggag tcacatgtcg gcatcagttt cccgacgatg
    50461 cgcatcctcc gccatcagtc ccggatggct tatcactgac acaacagcac cttagcgaat
    50521 cgcgggacgc gactcagtag ccttgccgtg tatttatcac ggcgaggtat tcatgaccat
    50581 caccacagac accactcttt tacacgaccc gcgtcgtcag gcggcgctgc tgtactggca
    50641 gggattttcc gtgccgcaga ttgccgccat gttgcagatg aaacgcccga cggtgcagag
    50701 ctggaaacag cgcgacggct gggacagcgt tgcccccatc agccgtgtcg aaatgagtct
    50761 ggaagcgcgg ctgacccagc tcatcatcaa accgcagaaa accggcgggg acttcaagga
    50821 aattgacctg ctgggacgcc agattgaacg actggcacgg gtcaaccgtt acagccagac
    50881 cggcaacgag gcagacctta atccgaacat tgctaaccgc aacaaaggtg ggcgtcgcaa
    50941 accgaaaaag aattttttca gtgacgaagc catcgaaaag ctggagcaga ttttctttga
    51001 gcagtctttc gactatcagt tgcactggta tcgcgccggg cttgagcacc gcatccgcga
    51061 tatcctgaaa tcccgccaga ttggcgcgac gttttatttt tcccgcgagg cgctgctgcg
    51121 cgccctgaaa accggtcata accagatttt tctgtcggcc agtaaaacgc aggcgtatgt
    51181 gttccgcgaa tacatcatcg cctttgcccg gctggttgac gttgacctga ccggtgaccc
    51241 gattgtcctg ggcaataacg gcgcaaaact gatttttctc ggcaccaact ccaacaccgc
    51301 acagagccat aacggcgacc tgtacgtcga cgagattttc tggatcccga attttcaggt
    51361 actgcgtaag gtggcatcag gtatggcctc acagagtcac ctgcgttcga cctatttctc
    51421 caccccgtcc acgctggcgc acgacgccta cccgttctgg tcgggtgaac tgtttaaccg
    51481 gggacgcgcc agcgccgccg aacgcgtgga aatcgacgtc agtcataacg cccttgccgg
    51541 tggacttctc tgtgcggacg gccagtggcg gcagattgtc accattgagg acgccctgaa
    51601 aggcggctgc acgctgttcg acattgagca gctcaaacgc gaaaacagcg ccgacgattt
    51661 taaaaacctg ttcatgtgtg aatttgttga cgacaaggcg tcggtgttcc cgttcgagga
    51721 gctgcaacgc tgcatggtcg acacgctgga agaatgggaa gactatgcgc cgtttgccgc
    51781 gaatccgttc ggctcccgcc cggtatggat tggttacgac ccgtcacacc gtggcgacag
    51841 cgccggatgc gtggtactgg caccgccggt ggtggccggt ggcaaattca gaatacttga
    51901 gcgtcaccag tggaaaggca tggactttgc cactcaggct gaatccatcc gcaaactcac
    51961 cgagaaatac aacgtcgaat acatcggtat tgatgccacc ggcctcggtg tcggcgtgtt
    52021 ccagctcgtg cgctcgttct atcccgccgc acgcgacatc cgctacacgc cggaaatgaa
    52081 aaccgcaatg gtgctcaagg caaaagacgt catccgccgt ggctgtctgg aatatgacgt
    52141 cagcgccacc gacatcacca gctcgtttat ggctatccgc aagaccatga ccagcagcgg
    52201 acgcagcgcc acctatgagg ccagccgcag cgaggaagcc agccacgccg acctcgcctg
    52261 ggcgaccatg cacgccctgt taaatgagcc actcaccgcc ggtatcagca ccccgctgac
    52321 atccaccatt ctggagtttt actgatgagc aagaaaaaag ggaaaacacc gcgaccagcg
    52381 gcaaaaacaa tgaccgccag cgccccgaaa atggaggcat tcacctttgg tgagccggtg
    52441 ccggtactcg accgccgtga cattctggat tacgtcgagt gcatcagtaa cggcagatgg
    52501 tatgagccac cggtcagctt taccggtctg gcaaaaagcc tgcgtgctgc cgtgcatcac
    52561 agctcaccga tttacgtcaa acgcaatatt ctggcctcaa catttatccc gcatccgtgg
    52621 ctttcgcaac aggatttcag ccgctttgtg ctggattttc tggtgttcgg taatgcgttt
    52681 ctggaaaagc gctacagcac caccggtaag gtcatcagac tggaaacatc accggcaaaa
    52741 tatacccgcc gtggtgttga ggaggatgtt tactggtggg tgccgtcctt caacgagccg
    52801 acacctttcg cgcccggctc cgtgtttcac ctgctggagc cggatattaa tcaggagctg
    52861 tacggcctgc cggaatatct cagcgccctt aactctgcct ggctgaatga gtcggccacg
    52921 ctgttccgcc gcaagtatta cgaaaacggc gcgcatgccg gatacatcat gtacgtcacc
    52981 gatgccgtgc aggatcgcaa cgatatcgaa atgcttcgcg aaaacatggt gaagtcgaaa
    53041 ggccgcaata actttaaaaa tctgtttctc tatgccccac aggggaaagc cgacggcatt
    53101 aaaattatcc cgctcagtga agtggcaacg aaggacgatt tttttaatat caaaaaagcc
    53161 agcgccgctg acctgctgga cgcgcaccgc atcccctttc agttgatggg cggcaagccg
    53221 gagaacgtcg ggtcgctggg tgatattgag aaagtggcaa aggtctttgt ccgcaatgag
    53281 cttatcccgt tacaggacag gatccgcgag ataaacggct ggctcggtca ggaggtcatc
    53341 cgctttaaaa actactcact ggacactgac aacgactgaa catcgccgcc tgcgggcggc
    53401 ttttttacac cccgtcatca cgccctcaca cgctcaccac cgcacaaaac accccgcaga
    53461 cacaccaacg cctcaacggg cagactaagc gccgtcacga cgcgctcaga cgctgaaaaa
    53521 ataaaatcag caccaccgcc agcgcgcagt gctttccccg cctcgcccgc ccgcttcatg
    53581 cagggcttta aatgcagtgc agcagtaatg gttagaatgc gccagcactg atgttagata
    53641 ccagaactgg catttaaaaa tgaatgcaaa taaatgcacc aaactaatta cttactgaca
    53701 aaaaataagt aaaatcaggc gcaagttggc attgagaccc ctttcagggt attaggaata
    53761 attatttcag gagaaaaaat catcacttcc tc
//
LOCUS       DAZGHF010000016        52108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGHF010000016 DAZGHF010000000
VERSION     DAZGHF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.016"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..847
                     /locus_tag="ACHK3A_001072"
     CDS             <1..847
                     /locus_tag="ACHK3A_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739188.1"
                     /translation="ITAFSIISIFIHSYVTCAIRLFRKQKSRLNNTVRYEKLSRKKGF
                     NFCLSLQLIIPGVVQILPMMFLFNQLNFSEGTSDWYEMSILIAITVVIVTIGILPGII
                     HINERQNGNKMTGIIVVLIFIPVATAALSVWYRPIPNMIANMTMNLSGISDQRTHEYY
                     IERATHPAGMFNGKIWNTRYYKNIPDRFFITGVNTFTLGNIKLICPTAIVKARIESLK
                     FTVNDIDEYEQKGKKLKKTAMKCIPFDKNDIHTWDSPLSEPIYYEKIKQTIDNSMLKI
                     LHVVK"
     gene            915..1223
                     /locus_tag="ACHK3A_001073"
     CDS             915..1223
                     /locus_tag="ACHK3A_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO8739189.1"
                     /translation="MQSQTALLLLIIPFPVLVIASPESTQLALALKQLDGVKTSLEHA
                     RTSASNDPQARYFFDYLQAQQDISVVEQGIRRYLAPARAQPRAIQPLSANYQQENPDD
                     "
     gene            1216..1449
                     /locus_tag="ACHK3A_001074"
     CDS             1216..1449
                     /locus_tag="ACHK3A_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO8739190.1"
                     /translation="MTDSQLAAFQAASLSKPETVSLLVLGLFTSVLLLWCAWTLMVAW
                     QGVCRQRLSWQSFGAVAAHSLLLVLVSFWLVLS"
     gene            1459..1776
                     /locus_tag="ACHK3A_001075"
     CDS             1459..1776
                     /locus_tag="ACHK3A_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO8739191.1"
                     /translation="MRWFLLLLFATFNALADLPTMEDPSRGQGKGIMETLQNYGFDIV
                     VLLALAICAVGFLVVANNCIATYSEIQSGRKQWKDLGSMAGVGSILLVVIIWLLNQAS
                     DVL"
     gene            1778..2131
                     /locus_tag="ACHK3A_001076"
     CDS             1778..2131
                     /locus_tag="ACHK3A_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO8739192.1"
                     /translation="MATIPFLPDRLNREPVVWRGLTTSELFLALALGLGGGCAFGILL
                     ALITHYWPLIPGSALSGAALLIQGGGRILARAKRGKPDTWLPQALLAWLERHHLRGAQ
                     LIQHSAVWVVRRSPR"
     gene            2128..2787
                     /locus_tag="ACHK3A_001077"
     CDS             2128..2787
                     /locus_tag="ACHK3A_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO8739193.1"
                     /translation="MSRFRHALAERDNHILTLRAVIVLLCLCLAGVGYGWYSAPQKLT
                     LWFPPDLRSGSTRPWWEVPPASVYAFTFYIFQQLNRWPDNGEEDYSRNLRSLRAYLTP
                     ACYTRLEHDFQVRRNGGELRDRTRGVYELPGHGYSSLVHEGEKRPFPRVEALSRDSWR
                     VTLDVAVEEYYQNEPVKKALVRYPLQVVRYAIDAEKNPWGLAIDCTDAAPQRLVAEEK
                     P"
     gene            2784..3551
                     /locus_tag="ACHK3A_001078"
     CDS             2784..3551
                     /locus_tag="ACHK3A_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO8739194.1"
                     /translation="MKPVLFTLLFSFPAFATEVVQWENIPLPVTLHVGQERIVDVGKP
                     VRIGYPATLEGKVRLQSAGGKVFLLANTAFPTTRIQLRDTGNGELILLDIHATQGTST
                     LEPMQISYTPQTPATAKTSVPVTASTEPLPVLLVRYAAQNLYAPLRTVEALPGVTPAP
                     VRLAKRITTLLPQQPVTATPLASWQVNATTVTAIRLQNHSGQRITLDPRELQGQFTAA
                     AFQHDWLGPRGLAEDTTVAYLVTEGPVSRALLPEPKP"
     gene            3548..4843
                     /locus_tag="ACHK3A_001079"
     CDS             3548..4843
                     /locus_tag="ACHK3A_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO8739195.1"
                     /translation="MMPRQSLLLKILLPLIVLVTVWLLLRPASKNTQETSLSRTVAAL
                     TPQELRDLGIDGDTPADTVATLVGQMKQYRRELQAIQTNNESQQAREKRLNAQQATLA
                     SQLREDMRRQQDQMRDELVKSQRGLLDKLDRLMAKRPDDIPPGLGLTPGELPADVQPD
                     IRWLEPLDAPPPVDEKLLRVPLKAADKPPPKPDVKVYTLPQNATLTGSVAMTALIGRV
                     PLNGTVSDPYPFKLLIGEDNLTANGVELPDVAGAVVSGIASGDWTLSCVRGQITSMTF
                     VFHDGIIRTVPQGGKSGNKQQEIGWLSDNQGLPCIPGERKSNAKEYLTSQFLLAGSGA
                     AAQAFANGESTTVVEDGSITSAVTGNNGQYVLGQAIGGGLKETADWMKQHYGQMFDAV
                     YVPPGHKVAVHLTEALSIDYEPEGRQVKYQQASTPGGLE"
     gene            4840..5199
                     /locus_tag="ACHK3A_001080"
     CDS             4840..5199
                     /locus_tag="ACHK3A_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO8739196.1"
                     /translation="MKFTILILLLLLAGCSTSQEELMPVDPNASMLTLWQQQSGGNAA
                     LLDARAQLRRPPDGPVAQPEADTIRSQFPRLPNPDMVLYVFQHLNSNTPVPGYSTVFP
                     FYTRPQYALPGERLTPP"
     gene            5196..7838
                     /locus_tag="ACHK3A_001081"
     CDS             5196..7838
                     /locus_tag="ACHK3A_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO8739197.1"
                     /translation="MNERRGLTEQALNTSYRKGPSFVDWLPWVEFLPESQTLLLEDGR
                     SVGAVFELYPVGTEGRSTAFLEQVRDTVTDALQDAFDEHERAPWVVQFFCQDEDDSEA
                     WLASLASYAAPEIRDSAFTREWLTQMANHMVNLTRDEGLFLDNTVTRTRWRATWRRTR
                     MVVYRWLPGLSPDEPSPEETLNNICERLTAGLKAAGISAIRQNEQDIHRWLVRWFNPA
                     PPAEDKKAFWQQLDLPETEPLPVLADFAERLLASEPRSDAANGVWWLDNRPHKVVVVD
                     RLRKAPPPGLLTGETARGDGINALVDLLPGGTIMNLTVVVYPQDKLEQHISRLADRAI
                     GDNTDSEYTRRDCSEVRSFLKDGHKLYRSTLAFYLSAPDTDILFRQQRQLQSLLLNAG
                     LVPVQEQHEVAPLSHWLRWLPMCFDPLRDPRGLYTRLNFVQHIAGLLPLYGRETGTGH
                     PGISYFNRGGTPLCFDPLNKLDRAQNGHLLLLGPTGAGKSATLNAKIAQLMALHRPRL
                     FIIEAGNSFGLMADYAAAHGLTVNKISLRPGSGVTLPLFADAWRLVEQAVTDAEPPED
                     EESDGDDQRDVLGEMEITARLMITGGEAKEDARLTRPDRAMIRQAIIAAAHTCYEEKR
                     QTLTQDIRDALFALSRDNSLPEARRQRAYEMAESINLFCAGFEGELFNREGQMWPEAD
                     ITLVDLATFAREGYEAQLAISYISLINHINNLGERAQHLARPIVNITDEAHIITVNPL
                     LARFLTKGSKMWRKLGIWLWLATQNLADFPDEAKKLLNMIEWWELLVMPPEEIEQVCR
                     FKALTEEQSQLLRSAVKSPGQYTEGVVLSPRIEALFRVVPPALYLALAMTEKHEKADR
                     MRIMKETGCSEVEAAITLSSVISQ"
     gene            7887..8807
                     /locus_tag="ACHK3A_001082"
     CDS             7887..8807
                     /locus_tag="ACHK3A_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739198.1"
                     /translation="MTAIACWFNREESDSIWAVSDSRITQQNSRLTDHCPKLFSIPVS
                     VIRSSDVYRIHPQKIFEFGFGFAGSTMIGINVKEMLAVSLARLHEIANNNPEQQISTE
                     AFPSLNEIASLAKNIAEQYMSDIGQSFPQAVRIQMIIFGFCWRTQTYKIIKLNNSIEH
                     PANLQIEDIQNLSTGRPVLLGDNLHELNEFIENTRQQFTPNTINWWRTPFIALNNWIN
                     QETVNTIGGYIQMVTAHPLGTRISFLTNLNENIIRTSYAGINTTESFGPTIGGFILMP
                     MDGMSLPDENGWDVGNQVTRTAADRVATNR"
     gene            complement(8810..9034)
                     /locus_tag="ACHK3A_001083"
     CDS             complement(8810..9034)
                     /locus_tag="ACHK3A_001083"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8739199.1"
                     /translation="MKNTSSIQAQFGAHLKKLRLQSGLSQEAFADKCGLDRTYVSGIE
                     RGVRNPTLEVISVLGKGLDMDIKELFNFER"
     gene            complement(9074..9337)
                     /locus_tag="ACHK3A_001084"
     CDS             complement(9074..9337)
                     /locus_tag="ACHK3A_001084"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8739200.1"
                     /translation="MEKTGSIKKVLGKRIRALRREMDLSQEALADKCGIDRTYMSGIE
                     RGVRNPTLVVICAISDGLGMDLSHLFMFDDGAEKDAESTKCDS"
     gene            9445..9720
                     /locus_tag="ACHK3A_001085"
     CDS             9445..9720
                     /locus_tag="ACHK3A_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8739201.1"
                     /translation="MTKKDWHPADIIAALHKRGTSLAKLSREAGLASSTLANALNRPW
                     PKGEWLIAIALGKHPSEIWPERYLNTGNKREPRKPLHLMTTTENSGT"
     gene            9939..10313
                     /locus_tag="ACHK3A_001086"
     CDS             9939..10313
                     /locus_tag="ACHK3A_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO8739202.1"
                     /translation="MMTYCARIVAIGLFVADGLADRTLITFDSNGPKDCLDYSLSLEP
                     SFREDDLLILPGDRLLLASHDYLVTAVGKEAQQSLFELGHLTMVFNGSLNACHSGAVH
                     LSGPIPKLHDLYGELLIEEGRP"
     gene            10357..11715
                     /locus_tag="ACHK3A_001087"
     CDS             10357..11715
                     /locus_tag="ACHK3A_001087"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8739203.1"
                     /translation="MELNRSLKARHLTMISVGGVIGAGFFLGAGAAIARTGPSVVLAY
                     LIGGVITLFIMLLLAEMAVARPVSGSFESYARTAFGPWCGFITGWTYWLAFLIGPASE
                     AIAAGTFLNLWFPGVPVWLFCLVIASSLTAINMVGVHFFGEVEFWLSLVKVVALLAFI
                     VAGCYSLGLDNASPASISHPTDSGSFFAGGLPGFLGAMLMVIFSFGGTEAIGTAAGES
                     ENPQRDIPRTLRGTVVRILLLYVGSLTVLLLVLPWQQAGVSSSPYVEASGILGGHMAR
                     HIMNFVVLTAALSCIDTGVYATSRMLHAMASDGYFPAWFARLHPAHKTPDNAILASSL
                     VLFTGVIIAILSPDAYVILAGISGFGFLFTWLMIALSQPTLHRIALHDGTLKYKAPAA
                     RYIQPLTVGLIALIMAGQFFTDGGGITLVTVLIWLALASAYYIFVVRKNRRLSDEKSH
                     AQ"
     gene            11693..12484
                     /locus_tag="ACHK3A_001088"
     CDS             11693..12484
                     /locus_tag="ACHK3A_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HFO8739204.1"
                     /translation="MKKVMLNELTGREIRELLQSGKELTAIVNFGSCENHADHLPLGP
                     DMFVPEIVACRVAQRVDNTLVLPCTPFGTSVHYNAYPLAVTLRYETNIALAEDIFISL
                     LHHGIRRILVINGHDGNIPAIEIAARNVKARHTQAAIVVVPAWWDLVGHHLGKGFFDS
                     WDNTGLGHGGEGETSVMMALIPELVELKYAQKQIPVEVFADYGVNRIWDIGEISATGA
                     TGDPRCATAEKGQRILACLVDVLCALLRKLDAANWQYALNKDANM"
     gene            complement(12587..13006)
                     /locus_tag="ACHK3A_001089"
     CDS             complement(12587..13006)
                     /locus_tag="ACHK3A_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739205.1"
                     /translation="MANQQAAKWAGVAAIAACLTFVVTTIGLLLAWRSLHQWKPQYKE
                     NSRLLLIEALIAFQKCLITIPKNLDNDPTYQSRKEFLKASTEVELRGQIYLKQHSNEK
                     LKDELANLRSKCAEFVGGKVTKPELSFISAIILLIEV"
     gene            complement(13245..14135)
                     /locus_tag="ACHK3A_001090"
     CDS             complement(13245..14135)
                     /locus_tag="ACHK3A_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning protein RepB C-terminal
                     domain-containing protein"
                     /protein_id="HFO8739206.1"
                     /translation="MISQCFDSRFYTFKVESLVYARTLPVNVKFSTKYKQIKASVDEI
                     GLIEPIVVYINDANDKVILDGHLRVEVLKELGITTAHCLISPVEDTYSYNKRVSRLTI
                     VQAQNMLLKAVESGVSVEKLCAVLGVTPDTLIGKLKISDGIAPEVMALLAEKNVPQTI
                     FSVLKKLKFYKQIEMVNTMISINNFTRKFAMSMLHAVAPEHLVAPKKDASRETDIRKN
                     LERLEKEMASVQVETQHLQDEYAENNLRLVIIKNHIERLLANGQVLNWLYDNHQEYLS
                     VLKQISGLDDLNKLGQTIEE"
     gene            complement(14137..15024)
                     /locus_tag="ACHK3A_001091"
     CDS             complement(14137..15024)
                     /locus_tag="ACHK3A_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO8739207.1"
                     /translation="MKSFDEQIELVDISDIYIANPRKRNKFIHNEIKENIKTIGLKRP
                     VSVRPVNNENYKYALICGQGRLEAYIEYGETKIPAIIKQVDEETAHLMSLAENIARRN
                     PRAAELMEDIRRMKTSGLTDREIGLRLGYNSSWVNGVITLLSSGEKHLLRAFETGDIP
                     LYLAVEISRSGDEETQELLTVALKEGHIKGGQINKIRRILEQRKKGDKGTISTAFFPG
                     KKQKKLTPEELANVYQENINEQKAILLKSTMVKEKILAVKQIFGYLLKDNDFVTLLHS
                     NNIHDIPSLLDGSDVSGEK"
     gene            complement(15024..16559)
                     /locus_tag="ACHK3A_001092"
     CDS             complement(15024..16559)
                     /locus_tag="ACHK3A_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8739208.1"
                     /translation="MSDIVAGNRIAAQYLRMSTEHQKYSPENQREYIEKYAKEHCITI
                     KYTYDDAGRSGLSLDNRPGLRSLLYDVENGIIKVSLILVYDVSRFGRFQDIEEAAHYS
                     WLLKRNGVRIIYCAEPFSEEQTELFMLGLSYSRYGAATYSKNLSQKVFLGQANLVRRG
                     YYQGGMPGYGIRRLLIDEHTNPKGMLLPGQRKSLQTDRVILIPGPAEEVVIVNKIYDM
                     FIDEGKPEFVIASELNRKNIPAENGLQWSRCKIHQILTNEKYIGNNVYNRTSGRLKTR
                     SVRNPQDQWIRCDGAWDPVVSVEKFEAAKRIIEARAVQYSDDELLDKLRHLLKLRGRL
                     SGIVIDEEELFPSSSIFRSRFGGLVRAYTLIGYHPRHDYSYLEIKKQIAECHLTFMDN
                     LVQDIYRYGGWAEMHQDGSLYINDEFFVSANLVRCQRLSSGKLRWKIKFNVSHYCDVM
                     IFPRLDSTNNSVVDYFMFPAIDVLYNDLTLHENNGFILELYRFDDLSCLENLVKRTGL
                     ESK"
     gene            complement(16583..17530)
                     /locus_tag="ACHK3A_001093"
     CDS             complement(16583..17530)
                     /locus_tag="ACHK3A_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8739209.1"
                     /translation="MRKILFFLLLMIAVLPEVQATCSFYGGVTSEVSGYLNFGNVVIQ
                     RDAQVGSTIATAITGAYNNGDGIAGCKREAWTARWELTQWGTLSGYGNGVYNTNLPGV
                     GLRLSAASTGKTLPYETSYAYNAGGTWVSIPGDGIKGELIKTGDITSGTLTDGVLARA
                     SVVNQFYFANVTLNGMNTVKSEACTVTTPSVDVPMGDHDKSEFSGAGSSTDWVSFDIG
                     LSCDVGARINVRIDATADASASSQGVMLIDSGGASGVGIQLHYRPDDAAVQYGQERFY
                     WQSVYGDEIVQLKARYYQTAGSITPGAANGTATFTMSYK"
     gene            complement(17549..18553)
                     /locus_tag="ACHK3A_001094"
     CDS             complement(17549..18553)
                     /locus_tag="ACHK3A_001094"
                     /inference="COORDINATES: protein motif:HMM:NF012635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8739210.1"
                     /translation="MRVWRGMILLAGILFCQASWAFCTSSVSFSISPGTVVVQRDAVV
                     GEPISDWLVSNQGMAYQDCNYDATPQYVINAGIKNTGSRASVTWNGEAVFNSNVAGVG
                     FVFEGRSSVDSNPLPENWIGIASGQSQVFTLQISHSVGYHPSINDQARIRLIKTGDIT
                     PGTLSGNLGRFIAGTRENGQWSTEIPITFSGGQITTIACSVTTPDVNVPLGQHQKAEF
                     SGPGTGTGWQAFNIGLDCDKSARINVQIDAVQDPSNVAGVMKLDSAPGDMAATGIGVQ
                     LFFAPDNSAVQFGQSRYYYTSPNGGTETVQLKARYYQTAATITAGVANATATFTLTYK
                     "
     gene            complement(18550..21066)
                     /locus_tag="ACHK3A_001095"
     CDS             complement(18550..21066)
                     /locus_tag="ACHK3A_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8739211.1"
                     /translation="MNNPTPRLMIILLTLLLAIVLLSGYLRNAEAREVFNPHALEIDN
                     PGQPVADLSTFAESGGQLPGTYRVTVFVNGEKQSDAQDIAFIDGAKKKLSPQITPAML
                     KAWGVKTDVFPALAALPPDQPLDDIGRYIPMANTELRFSKLQLNVSIPQAAMNASARG
                     YVDPSEWDEGVPAALLNYNLSGSNTWRDGQNGSDDNYYANLQSGINLGAWRLRNYSTW
                     NYDEDNGSHWDSVNTYLQRDIQRLKGQLTLGDSYTPSDIFDSVQFRGAQLASDDNMLP
                     DSLRGFAPVIRGIAQSNAQVTIKQNGYIIYQSYVAPGAFTITDLYPTSGSGDLEVTIK
                     EADGSERTFVQPFSAVPIMQREGRLKYALTAGKYRSGNEDSDEPQFGQVTAIYGLPHA
                     ITVYGGTLYSDDYQSGAAGLGFGLGELGSLSADITAAHTTLNNDETHNGQSYRVQYSK
                     DFQTTDTSFTLAAYRYSTAGFYTFQEANDLRADDDNGWQMTYNKRQRLQLDLSQSIGS
                     YGSFFISGYQQDYWQEDGYERTLSAGWNGNIDGISYSVTWSYSDYPESTQPADQQLAF
                     NIQVPLSRFMPNAWASYSVNTAKHGDTRQQVGLNGTALADNNLSYSLQQSYTNHGVGG
                     SGNINADYKGGQGEITGGYNYDDDTQQVNYGLKGGIVAHPHGITLSQPLGQSLAIVKA
                     PGADDTKVQNNTGVYTDWRGYAVVPYVNPYKKNRIALDTSTLGDEVDTDTAVQTVTPT
                     QGAVVMADFNTRVGRRVLMTLLYRGLPVPFGAEAKLKEGGSGIVGDDGQVYLTGVPEE
                     GDIAVNWNGAQQCVVHYRLPEQENQPSVILVNQECREVAK"
     gene            complement(21078..21758)
                     /locus_tag="ACHK3A_001096"
     CDS             complement(21078..21758)
                     /locus_tag="ACHK3A_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8739212.1"
                     /translation="MNRYADVVLALLLAFLSVQAQAGIVIGGTRVIYDGDKKETAASV
                     RNPEKSGVYLVQSWVDSGEQGGKTPFIVTPPLFRINPGEENMLRIVRTGGNLPQDRES
                     VFWLNVKSIPATDDSHSRTNVLQVVVKSRLKLFYRPAGLEGQPESAYHQLSVAHSGNH
                     LTVSNPTPYYVTLFTLKVDGQEIKEADMVPPKGSVSFTLPSATASAVTWQAISDYGGV
                     SQTESRKL"
     gene            complement(21872..22384)
                     /locus_tag="ACHK3A_001097"
     CDS             complement(21872..22384)
                     /locus_tag="ACHK3A_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8739213.1"
                     /translation="MKKTMLSACFLLVAGNAVANDGTIHFTGSITDQTCTVDSGSQNL
                     NVDLGNVGQTALNGAAGMKASPTRFTLNLSGCPDTVTGANVKFDGTSDGVNQNLLALD
                     SGTGIATGVGIEIADKNGAVIPLHTASMDYPLAAGSNTLDFVARYVSTGTAVTTGTAN
                     GTSQFTINYK"
     gene            complement(22752..23342)
                     /locus_tag="ACHK3A_001098"
     CDS             complement(22752..23342)
                     /locus_tag="ACHK3A_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase"
                     /protein_id="HFO8739214.1"
                     /translation="MPEKRKHLTRHEVGQILLAADRGIHFERDYCLIQMCFLHGLRVS
                     ELCGMRLSDIDLIGRSVYVRRLKNSLSTQHPLFDNELPALMRWLAVRNRWRDADSDWL
                     FLSQKGGALSRHQVRLLLKRYGELAGVSISAYPHMLRHGCGYALADLGRDTRLIQDYL
                     GHRNIRHTVIYTATNTQRFMNVWGNVRENHTISARM"
     gene            23555..24478
                     /locus_tag="ACHK3A_001099"
     CDS             23555..24478
                     /locus_tag="ACHK3A_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO8739215.1"
                     /translation="MRAQYILLLLCAAFPLRAITTPEIAASALTPDCVKYRVVGLCYW
                     LYCTPFGCTVRTSVKVSHFRPDLVVSAYSNTGQNPWAEMSLLSPPLPGVAEGGGDTNP
                     RSASQHSKVRFKNADAIGFPAGDELAKFFTQFGYICSPSSQPFQPYFLSQPDTLAWRS
                     GVPEMTYPEALTPGMREVGQNGDMWGNIYPRAGAINQTHDYKAAAVIAQRAADLVTRT
                     GQPHLYTPLTASSRAGYWPPSPVIEGDSDNHRWQMLTPKKSAACSVFPDGSATDTYAN
                     KLAEDGAYAWTLWRPYKCCPRRGQTFLGSTG"
     gene            24490..25851
                     /locus_tag="ACHK3A_001100"
     CDS             24490..25851
                     /locus_tag="ACHK3A_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO8739216.1"
                     /translation="MRALFLVLLFFAGVCVAADDYSFRTRGAVDDLLYYQIGGGEAVM
                     PPPVRRSPSPLSLGLGWNADMMCGNFDIDTTVRNQLNGITDGFQQLMGNVIQSATGAV
                     ASLPAMIIQRANPQLYDLLTNGVLQGRLDFDKSLLNCQKMAEKMTDLAADSAWFSGAK
                     AENYQSLAASDNDAIRTDQKAAKEAAEKGKRWVGGEKRGGKGQLPIKVVHDATAAGWN
                     ILNQQPATSTTSLSSSECDGELCSTWTSPEEAAGWMTRVVGEQTISVAQVTDDPDARS
                     GAQAGIGLHPLIDEEKAKILPLLTDLVNGSQKPGFDNLAKASGGSMQLTRGVVEALRD
                     DPDVAVLTERLAGELALARVTEQALLARRTLLAGMREPNIANVKEAQESLGKTTAQLD
                     EELNQLKLELDFRQALTRNTASQILQRKQQRDQLQGQVVEVPDDSDSRLHNLNNPQPD
                     SPR"
     gene            25848..27350
                     /locus_tag="ACHK3A_001101"
     CDS             25848..27350
                     /locus_tag="ACHK3A_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO8739217.1"
                     /translation="MTANSYFEYVLTLLGWLVNNGVWQTLTATGLFAVPLLAKLVALW
                     LKARGQGADEGNVARLLVVWVEHSLYSALLVIFFAGVPFLNVDINTLTYDSQRTKQCG
                     WSVVQPGDSGYAPLINELSGQSASVPVWWYFTHALGKGITSAAVAVLPCKPDLRQLRF
                     EVQHTRLADPALAQELQDFVNDCYGPSRARLKRLSSDISEEQSREVDWVGSSFYLATL
                     GYYDRDHSRSPRAAWPYDASRDDGLSDTGGGGYPACKEWWSDGDTGLKNRLLKQVDTT
                     LWQQLQKLGQSKEEYEEAVLRSLVSPRNMQVSQGGLVYNGFGGSAGDNLSQTQLLSRV
                     GGIIGAGLSSYALFPAFDNVRQALPMVQAFLEMALVICIPVILLFSAWDLKTVITLSF
                     VQFALFFLTFWWELARWLDNWLMQMMYDSDTHSYFNLWGLQNTSDDLIVNIIMGVMFL
                     VLPAFWLGALTWAGIRIGAAVAGGMGSAVGDIRSAGEQVGKMIVSKTRIP"
     gene            complement(27495..27695)
                     /gene="glgS"
                     /locus_tag="ACHK3A_001102"
     CDS             complement(27495..27695)
                     /gene="glgS"
                     /locus_tag="ACHK3A_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006174923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO8739218.1"
                     /translation="MNRQDINALKNFDFLARSFARMHALGQPVDIDAVTGNMSDEQQA
                     WFRERYDHYRKQAERARVIELR"
     gene            28029..28319
                     /locus_tag="ACHK3A_001103"
     CDS             28029..28319
                     /locus_tag="ACHK3A_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739219.1"
                     /translation="MSLFDKKPSLMPVVIAPDVWQSAVEFPADPQQVEERLGNLLLVV
                     LLTLRTAGTSRKKVTFTIYCQPPQGDMKVPVALPLSLNYHGHYVQVSMAEQA"
     gene            28385..29290
                     /locus_tag="ACHK3A_001104"
     CDS             28385..29290
                     /locus_tag="ACHK3A_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFO8739220.1"
                     /translation="MDRSNDIYQKVTDQIIAELENGNVPWLRPWRDGEPPFPINALSG
                     RPYHGINVPLLWNSADRQSFASDRWLTFHQVSALGGQVRRGEKSSLAVLYLPRTQEET
                     DALGQPLLDKDGEPKVRHFGIIREFWLFNLSQCDGLPQALSEPFTRPATPVEMAEAIT
                     MNSGVTLRHRRQSKAYYKPATDLVMMPHPQQFESSEAYYATLLHELTHATGHASRLNR
                     PGIAGAERTEALYAAEELVEEMGSAFLCAHCGIQGRLQHASYIGYWLDILRQDKRAVI
                     RASGMARHASEWLLNAVPQPCQLSA"
     gene            29356..29727
                     /locus_tag="ACHK3A_001105"
     CDS             29356..29727
                     /locus_tag="ACHK3A_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739221.1"
                     /translation="MGLDLFFFERKKNAPDESQYARVGYFRKVNPLLKWVDKHVGLVR
                     NQVLLEVTKANLQALLSDLQSLTPDNCGAVYPTIDGFFYGYTDYGEHYWKDVRTVTLW
                     VKETLETFDFERDRLMFYAWW"
     gene            29901..31490
                     /gene="mobH"
                     /locus_tag="ACHK3A_001106"
     CDS             29901..31490
                     /gene="mobH"
                     /locus_tag="ACHK3A_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFO8739222.1"
                     /translation="MLKWLKRPSQSQPPTPDATLPEGWFAPQSAEQLLATPLRQSCLH
                     QLRQLTPLPDTLFDSWILAAIHRFAALVQLLPASQSHHHSTPGGLLDHSLEVACNAIR
                     LRQGYLLPPEAGAEEQARQASAWTVAVVCVALLHDTGKIAVDMEIHQRDGTRWHSWQG
                     YLAQPYRWQYQSHGRDYHLHPAATAMLINQLIPSPLLDWLAGYPAAFASLIYQLTGHY
                     ERSGALAELVQQADKASVARYLGGDIKTALAQKPSSFPQQLLTALRSLVQNEYRLNNP
                     ECGSDGWLTEDALWLISKTTADRVRAWLLQHGVSSVPENNNRLFDELLTHQLIVANED
                     KAIWRCIIRSDKGWSPVEPLTLLRLTPSVIWGQDDQRPACFDGDVTPVIEAEPAPELS
                     APTQPLPEIADDPRLGDNNNFLQWLKNHTSPSATVNTRNGKVHVVENHLFLISPGIFK
                     TYAAETTGDSGDGWKLAQRMFQESGLALRCNDDSFIWICEVRVPQKKSSQLKGFLMED
                     PKQVLGEKVPVNNPWLKLLRE"
     gene            complement(31546..32199)
                     /locus_tag="ACHK3A_001107"
     CDS             complement(31546..32199)
                     /locus_tag="ACHK3A_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO8739223.1"
                     /translation="MKKKLPDQLSRECIIAAMRAYDEGVRHQFSESRLYDVVFEGRSY
                     PPKAIVGIAASNMTGVEFSPEDFSGGIKSKCVKLLTDQGFQIVEKHLGNEQPAISIFP
                     DELPPQSVFTEGAATQVTVNRYERDRKARQAALAWHGCLCKVCGIDMMKVYGEIAKGF
                     IHIHHLIPLSDIKENYSLDPKKDFIPVCPNCHAMLHRRNPPFSPEELKSIIEYHAAL"
     gene            complement(32311..32577)
                     /locus_tag="ACHK3A_001108"
     CDS             complement(32311..32577)
                     /locus_tag="ACHK3A_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005289972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739224.1"
                     /translation="MSPRTHRQLVSVEVMWPAQTLPLPLQQVVEALNQGETPDQIIIR
                     MNQRGLLAWREDASAQDTHDIFQVRLDNQNEARFLCRYVTLPLH"
     gene            complement(32704..33045)
                     /locus_tag="ACHK3A_001109"
     CDS             complement(32704..33045)
                     /locus_tag="ACHK3A_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012776626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8739225.1"
                     /translation="MNVTSPKMLSQAIRDARKQRNLTQRETAASMGMKQSTVSEFENH
                     PEGTSLDTLFKLLAALDLKLQVVSWRGKKTLLMAGSRSGDLWKYEQKGHGAIRTEPPF
                     SLAYHLDFQST"
     gene            complement(33456..33944)
                     /locus_tag="ACHK3A_001110"
     CDS             complement(33456..33944)
                     /locus_tag="ACHK3A_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8739226.1"
                     /translation="MAKKTAPLVPGTNQLLAEFGERLKLARLRRKLTAKRVAERAGMS
                     PMTLRSLESGGTGVTIGAYLSVMQILGLEKDLNKLAAEDEQGRQLQDSRLINKTRVSR
                     PAAISHTNRHTGNVIKNDAFDKQFSTSQEKSIDSEVKTEVVTSASLAALLKPIKKPRK
                     GS"
     gene            complement(34252..35061)
                     /locus_tag="ACHK3A_001111"
     CDS             complement(34252..35061)
                     /locus_tag="ACHK3A_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFO8739227.1"
                     /translation="MTNIFTLMNPISSATETLLSQINRICTAQGIPFFIAGATAREIL
                     LHHIHSRSIGRRTRDIDIAVFVDSWPRFDALKNAFLAEGAEVVQGNAHRMIVKGIELD
                     IIPFGGVAEGNQIAWPPEREIIMAVDGFAEVFSHTVVVRLDNSENIPFCSLPGLALLK
                     LFAWRDRGHGSTKDATDLFKIICEYGVIENERIYESPVEGERLNWDPIRMGAFLLGSD
                     IATISEHSSLQDLLHLDKERLIDVIVRQHDVGDAGIEQIINDFWDGMASDG"
     gene            complement(35015..36013)
                     /locus_tag="ACHK3A_001112"
     CDS             complement(35015..36013)
                     /locus_tag="ACHK3A_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HFO8739228.1"
                     /translation="MKEEQLLSDAIENLPKGFHLQYEHSGRHGKHDAWGKLTGPDGES
                     VIFALEVKFIHRKESLMAVREQMVRFPADFPVLLVCNRLTSALAEYCADSQINFIDTA
                     GNARIQVPGLYVRIEGRYEKKPVSASSRFAEGVMKLLFVLLSSPETLNETYRSLAGKA
                     GISLGMVSKAFDYLEAQRYFRKSKKGRRLINEGELEALWLKDYATALRPKLDSLSLSA
                     PVSWDELTLASGEYWSGEIAAAKLSGGYLIPENGVIFTSHPLLQRRKELGLKPVPGGK
                     LQLIAGFWGNFVLNHKAEAMLCVAELLASGDDRNWETARIINDKYLHLNESDLFSY"
     gene            complement(36641..37888)
                     /locus_tag="ACHK3A_001113"
                     /pseudo
     CDS             complement(36641..37888)
                     /locus_tag="ACHK3A_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(38111..38195)
                     /locus_tag="ACHK3A_001114"
     tRNA            complement(38111..38195)
                     /locus_tag="ACHK3A_001114"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38159..38161),aa:Leu,seq:caa)
     gene            38391..39410
                     /gene="ahr"
                     /locus_tag="ACHK3A_001115"
     CDS             38391..39410
                     /gene="ahr"
                     /locus_tag="ACHK3A_001115"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO8739229.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTILNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            39540..41042
                     /gene="yjgR"
                     /locus_tag="ACHK3A_001116"
     CDS             39540..41042
                     /gene="yjgR"
                     /locus_tag="ACHK3A_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO8739230.1"
                     /translation="MSEPLLIARTSDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEEGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLPWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(41161..42243)
                     /gene="lptG"
                     /locus_tag="ACHK3A_001117"
     CDS             complement(41161..42243)
                     /gene="lptG"
                     /locus_tag="ACHK3A_001117"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO8739231.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(42243..43343)
                     /gene="lptF"
                     /locus_tag="ACHK3A_001118"
     CDS             complement(42243..43343)
                     /gene="lptF"
                     /locus_tag="ACHK3A_001118"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO8739232.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(43406..43456)
                     /gene="ytgA"
                     /locus_tag="ACHK3A_001119"
     CDS             complement(43406..43456)
                     /gene="ytgA"
                     /locus_tag="ACHK3A_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO8739233.1"
                     /translation="MSIIILATISTTQENK"
     gene            43610..45121
                     /gene="pepA"
                     /locus_tag="ACHK3A_001120"
     CDS             43610..45121
                     /gene="pepA"
                     /locus_tag="ACHK3A_001120"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO8739234.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYRGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            45255..45698
                     /gene="holC"
                     /locus_tag="ACHK3A_001121"
     CDS             45255..45698
                     /gene="holC"
                     /locus_tag="ACHK3A_001121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO8739235.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            45698..48553
                     /gene="valS"
                     /locus_tag="ACHK3A_001122"
     CDS             45698..48553
                     /gene="valS"
                     /locus_tag="ACHK3A_001122"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO8739236.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLSADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(48607..49803)
                     /gene="yjgN"
                     /locus_tag="ACHK3A_001123"
     CDS             complement(48607..49803)
                     /gene="yjgN"
                     /locus_tag="ACHK3A_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO8739237.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVFLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLPANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            49996..50499
                     /gene="yjgM"
                     /locus_tag="ACHK3A_001124"
     CDS             49996..50499
                     /gene="yjgM"
                     /locus_tag="ACHK3A_001124"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8739238.1"
                     /translation="MNNIAPQSPVIRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(50545..50961)
                     /gene="rraB"
                     /locus_tag="ACHK3A_001125"
     CDS             complement(50545..50961)
                     /gene="rraB"
                     /locus_tag="ACHK3A_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO8739239.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            51123..>52108
                     /gene="argF"
                     /locus_tag="ACHK3A_001126"
     CDS             51123..>52108
                     /gene="argF"
                     /locus_tag="ACHK3A_001126"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO8739240.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMV"
BASE COUNT        12425 a        14166 c        13458 g        12059 t
ORIGIN      
        1 aattacagcc tttagtatta tttccatatt tattcacagc tatgttacat gtgcgattcg
       61 gctatttaga aaacaaaaat ctcgcttaaa taatacagtg cgatacgaaa aactatccag
      121 aaagaaagga ttcaacttct gcttatcctt acaacttata atacctggag tagtacaaat
      181 attaccaatg atgtttctgt ttaaccaact taatttcagt gagggtacta gtgactggta
      241 tgagatgagc atactcattg ccataactgt agttattgtt acaattggca ttctgcctgg
      301 tatcatacat attaatgaac ggcaaaacgg caataaaatg acaggaatta ttgttgtttt
      361 aatatttata cctgtcgcaa ccgctgcact tagtgtttgg tacagaccta ttcccaacat
      421 gatagctaat atgacaatga atctttcagg gattagtgac cagagaaccc atgaatacta
      481 tattgaaaga gcaactcacc cagcaggtat gtttaatggt aaaatatgga atactcgata
      541 ctataaaaac atccctgata gattcttcat tactggagtt aacacattta ctttagggaa
      601 tataaaactc atctgcccaa cagcaatagt caaagcaaga atagaaagtt tgaaattcac
      661 tgtaaatgac attgacgagt acgaacaaaa agggaaaaaa ctaaaaaaga cagcgatgaa
      721 atgtattccc tttgataaaa atgatataca tacctgggac tcgccattat ctgaaccaat
      781 ttattacgag aaaatcaaac agaccattga caactctatg ctaaaaattc ttcatgtagt
      841 gaaatgattt tttagctgct tcacctctga agctctccta ctttgctaat cattgagtat
      901 aaggagagct tcaaatgcag tcccagacag cactattact gttaataata cctttcccgg
      961 tactggtaat cgcctccccg gaatccaccc agctcgccct cgcgctgaag caactcgacg
     1021 gcgttaaaac ctcgctggag cacgcccgga ccagcgccag caacgatccg caggcgcgct
     1081 acttcttcga ctacttacag gcgcagcagg acatctctgt cgttgaacaa ggcattcgcc
     1141 gctatcttgc cccggctaga gcacagccgc gtgctattca gcccctgagc gccaactacc
     1201 agcaggagaa tcccgatgac tgactcgcaa ctcgccgcct ttcaggccgc cagcctgagc
     1261 aaaccggaga ccgtcagcct gctggtgctc ggcctgttta ccagcgtcct gctgctgtgg
     1321 tgtgcctgga cgctaatggt cgcctggcag ggcgtctgcc gccagcgtct gagctggcag
     1381 agctttggcg cggtggcggc ccattcactg ttactggtgc tagtcagctt ctggctggta
     1441 ctgagctgag gaggcatcat gcgctggttt ctgttactgc tgttcgccac atttaacgcc
     1501 ctcgccgacc tgccgacgat ggaagatccg tcacgcggtc agggcaaggg cattatggag
     1561 acgctgcaaa actacggttt tgacatcgtg gtgctgctgg cgctggccat ctgtgccgtc
     1621 ggttttctgg tagtggcgaa caactgtatt gctacctatt cggagatcca gagcggacgc
     1681 aagcagtgga aagatctggg ctcgatggca ggcgtcggca gcattcttct ggtggtcatc
     1741 atctggctgc tcaaccaggc ttcggacgtc ctgtgatatg gcaaccatcc cctttctccc
     1801 cgaccgcctc aaccgcgagc ccgtggtctg gcgcggcctc accaccagcg aactgtttct
     1861 ggcgctggct ctcggtcttg gcggcggctg tgcgttcggc attctgctgg ccctgataac
     1921 ccactactgg ccgttgatcc ccggttcggc actttctggc gcggcattac tgattcaggg
     1981 tggtgggcgg attctggccc gcgcaaaacg cggtaagccc gacacctggc tgccgcaggc
     2041 gctcctcgcc tggctggagc gtcatcatct tcgcggcgca caactgatcc agcacagcgc
     2101 cgtgtgggtg gtgcgcagga gtccacgatg agccgctttc gccatgcgct ggctgagcgc
     2161 gataaccata tcctgacgct gcgggcggtg attgttctgc tgtgcctgtg tctggcgggc
     2221 gtcggctacg gctggtacag cgcgccgcaa aagctcaccc tctggttccc gccggatctg
     2281 cgcagcggca gcacccgccc gtggtgggaa gtgcctcccg ccagcgtcta cgccttcacc
     2341 ttttatatct tccagcagct taaccgctgg ccggataacg gcgaggagga ctattcccgt
     2401 aatcttcgtt cgctgcgtgc ctacctgacg cccgcctgtt acacccggct ggagcacgat
     2461 tttcaggtgc gtcgcaacgg cggtgaactg cgcgaccgca cgcgcggcgt ctatgaactg
     2521 cccggccacg gctacagcag cctggtgcat gagggcgaga agcgcccctt cccgcgcgtg
     2581 gaggcgctga gccgggattc atggcgggta acgctggatg tggcggtgga agagtactac
     2641 cagaacgagc cggtgaaaaa agcgctggtg cgctacccgt tgcaggtggt gcgctacgcc
     2701 atcgatgcgg agaaaaatcc gtggggactg gcgatcgact gtaccgatgc cgcgccgcag
     2761 cgtctggtgg cggaggagaa accatgaagc cagtcctctt tactttattg ttcagtttcc
     2821 ccgccttcgc cacggaagtg gtgcagtggg aaaacatccc gctgcccgtc acgctgcatg
     2881 tcgggcagga gcgaattgtt gacgtgggta aaccggtgcg cattggctat cccgccacgc
     2941 tggaaggcaa agtgcgtctg caaagcgcag gcggcaaggt cttcctgctg gcgaacacgg
     3001 cgttcccgac cacccgcatc cagttacgtg ataccggcaa cggtgagctg atcctgctgg
     3061 atattcatgc cacccagggc acttcgacac ttgaaccaat gcaaattagc tatacgccgc
     3121 agacgcctgc cacagcaaaa acatccgtcc cggtgacagc atcaacagaa cctctgcccg
     3181 tcttgctggt gcgctacgcc gcgcaaaatc tgtatgcgcc gctgcgcacc gtcgaagccc
     3241 tgcccggcgt cacacctgcg cccgtccggc tggcgaaacg catcaccact ttactgccgc
     3301 agcagccggt taccgccacc ccgctggcgt catggcaggt caacgccacc accgtcaccg
     3361 ccatccgcct gcaaaatcac agcggacagc gcatcacgct cgatccgcgc gagcttcagg
     3421 ggcagttcac cgctgccgcg ttccagcatg actggctcgg cccgcgtggt ctggcagaag
     3481 ataccaccgt ggcgtacctg gtgacggaag gtccggtttc ccgcgccctg ctgccggagc
     3541 ccaaaccatg atgccgcgcc agagcctgct gctgaaaatc ctgctgccgc tcatcgtact
     3601 ggtaacggta tggctgctgc tgcgcccggc cagcaaaaac acgcaggaga cttccctgtc
     3661 ccgcaccgtg gcggccttaa cgccgcagga actgcgcgat ctgggcatcg acggcgacac
     3721 gcccgccgac accgtcgcca cgctggtcgg ccagatgaaa cagtaccgcc gcgaacttca
     3781 ggcaatccag acaaacaacg aaagccagca ggcgcgcgaa aaacggctta acgcacagca
     3841 ggccacgctg gcctctcagc ttcgggaaga catgcgccgc cagcaggacc agatgcgcga
     3901 tgaactggtg aaaagccagc gcgggctgct ggataagctc gaccgtctga tggccaagcg
     3961 cccggacgat atcccgcccg gtctggggct gacgcccggc gaactacctg ccgacgttca
     4021 acccgatatc cgctggctgg agccgctgga tgcgcctcca cctgtggatg aaaagctgct
     4081 gcgcgtccct ctgaaagcgg ccgacaaacc accgccgaaa cctgacgtga aggtctacac
     4141 cctgccgcag aacgccacgc tgaccggatc ggtggcgatg acggcgctga ttggccgcgt
     4201 gccgcttaac ggcaccgtca gcgatcccta cccgtttaaa cttctgattg gcgaggacaa
     4261 cctcaccgcc aatggcgttg agctgccgga cgtggcgggc gcggtggtct ccggcatcgc
     4321 ctcgggagac tggacgctct cctgtgtgcg cgggcaaatc acctcgatga cctttgtgtt
     4381 tcacgacggc atcatccgca ccgtcccgca gggcggcaaa tccggcaaca aacagcagga
     4441 gatcggctgg ctttcggata accaggggct gccatgtatt cccggcgagc gtaaaagcaa
     4501 tgcgaaggag tacctgacct cgcagttcct gctggcgggt tccggtgcgg cggcgcaggc
     4561 cttcgccaat ggcgaaagca ccacagtggt cgaggacggc agcatcacca gcgcagtgac
     4621 gggcaacaac ggtcagtacg tgctcggcca ggctattggc ggcgggctga aagagaccgc
     4681 cgactggatg aaacagcact acggccagat gttcgacgcc gtgtacgtgc cgccgggcca
     4741 caaggtggcg gtgcatctca ccgaagcgct gtctatcgac tacgagccgg aaggccgtca
     4801 ggtgaaatac cagcaggcca gcacaccggg aggtctggaa tgaaatttac catccttata
     4861 cttctgttgt tactggccgg atgcagcacc tcgcaggagg aactgatgcc tgtcgacccc
     4921 aatgccagca tgctgacgct gtggcagcag caaagcggcg gcaacgcggc tctgctggat
     4981 gcccgcgccc agctacgccg cccgccagat ggtccggtgg cgcagcctga ggcagacact
     5041 atccgcagcc agtttccgcg cctgcccaac ccggatatgg tgctttacgt cttccagcac
     5101 ctcaacagca acacgccggt acccggctac agcaccgttt tccccttcta tacccgtccg
     5161 cagtatgcgt tgccgggcga acgcctgacg ccgccatgaa tgaacggcgc ggcctgactg
     5221 aacaggcgct gaataccagc taccgcaagg gcccgtcatt tgtcgactgg ctgccgtggg
     5281 tggagttttt gccggagtcg cagacgctgc tgctggaaga tggccgctcg gtaggtgccg
     5341 tatttgagct atatcccgtt ggcaccgaag gccgctcaac ggcgtttctg gaacaggtcc
     5401 gcgataccgt caccgatgcc ttacaggatg cgtttgatga gcatgagcgc gcaccgtggg
     5461 tggtacagtt tttctgtcag gatgaggacg acagcgaggc gtggctggca tcacttgcct
     5521 cttacgccgc accggaaatc cgcgactccg cctttacccg cgagtggctc acacagatgg
     5581 caaaccatat ggtgaatctc acccgcgacg agggactgtt tctcgataac accgtcaccc
     5641 gtacccgctg gcgggcgacg tggcggcgca cgcggatggt ggtgtaccga tggcttcctg
     5701 gactttcacc ggatgaaccg tcgccggaag agacgctgaa taatatctgc gaacgcctta
     5761 ccgccgggct aaaagcggcg ggtatcagcg ctatccgcca gaatgagcag gacattcacc
     5821 gctggctggt gcgctggttt aatcccgcgc cgcctgctga ggacaaaaaa gccttctggc
     5881 agcagcttga cctgccggaa actgaaccgc tgccggtcct ggccgatttc gccgaacgcc
     5941 tgctggcgag tgaaccccgt tcggatgcgg caaacggcgt ctggtggctg gataaccgcc
     6001 cgcataaagt ggtggtggtt gaccgcctgc gcaaagcgcc gccgcccggc ctgctgaccg
     6061 gggaaaccgc gcgcggagac ggcattaacg ccctggtcga cctgctgcct ggcggaacca
     6121 tcatgaacct gaccgtggtg gtgtatccgc aggacaagct ggagcaacac atcagccgcc
     6181 tggccgaccg ggcgattggc gacaataccg attcggaata cacccgccgc gactgcagcg
     6241 aggtgcgaag cttcctgaag gacggccata aactctaccg cagcacgctg gcgttttatc
     6301 tcagcgcgcc ggacaccgat atcctgttcc gccagcagcg tcagctacag agcctgctgc
     6361 tcaacgccgg gctggttccg gtgcaggagc agcacgaagt cgccccctta agccactggc
     6421 tgcgctggct gccgatgtgc ttcgatcccc tgcgcgaccc gcgcgggctg tatacccggc
     6481 tcaactttgt gcagcatatc gccgggctgc tgccgctgta cggacgggaa accggcaccg
     6541 ggcatccggg gatcagctac ttcaaccggg gcggcacgcc gctgtgcttc gatccgctga
     6601 acaagctgga tcgtgcccag aacggacacc ttttacttct tgggccaacc ggagcgggca
     6661 aatccgccac cctcaacgcg aaaatcgcgc aactgatggc gctacatcgt ccgcgcctgt
     6721 ttatcatcga ggccggaaac tcatttggcc tgatggccga ctacgccgcc gcccacggcc
     6781 tgacggtgaa taaaatcagc ctgcgccccg gttctggcgt caccctgccc ctgttcgccg
     6841 atgcgtggcg actggtggaa caggcagtta ccgacgccga accgccagag gacgaagaga
     6901 gcgacggcga tgaccagcgc gacgtgctgg gtgaaatgga gatcaccgcc cggttgatga
     6961 ttaccggtgg tgaagcgaaa gaggatgcgc gcctcacccg gccggatcgg gcgatgatcc
     7021 gccaggcgat tatcgctgcc gcgcacacct gctacgagga aaaacgacag acgctgaccc
     7081 aggatatccg tgacgcactg ttcgcccttt cccgcgacaa cagtctgccg gaagcccgcc
     7141 gccagagggc gtatgaaatg gcggaatcca tcaacctgtt ctgcgccggg tttgaaggcg
     7201 aactgtttaa ccgtgaaggc cagatgtggc cggaggcgga tatcacccta gtggatctcg
     7261 ccacctttgc ccgcgaaggc tatgaagcgc agcttgccat cagctatatc tcgctgatta
     7321 accatatcaa taacctcggc gagcgcgccc agcatctggc gaggcctatc gtcaatatca
     7381 ccgatgaggc gcatatcatc actgttaatc ccttattggc gcgctttctc accaaaggct
     7441 cgaagatgtg gcgcaagctc ggtatctggc tgtggctcgc cacccagaac ctggcggatt
     7501 ttcctgatga ggcgaagaag ctgctcaata tgatcgaatg gtgggaacta ctggtgatgc
     7561 cgccggaaga gatcgagcag gtctgccggt ttaaagcgct tacggaagaa cagagccaac
     7621 tgctgcgctc agcggtaaaa tcaccggggc agtataccga gggcgtggtg ctttcaccgc
     7681 gtatagaggc actgtttcgg gtggtgccgc cagcgctcta tctggcgctg gcgatgacag
     7741 agaaacatga aaaggccgat aggatgcgga ttatgaagga gacagggtgt agtgaagtgg
     7801 aggcggcgat aaccctaagc tcggtaatct cgcaataaaa atatgctaag ctattcgcat
     7861 taatgttaaa taactggaaa aaccttatga ccgcaattgc atgttggttt aatagagagg
     7921 aaagcgatag tatttgggca gtcagcgact caagaatcac tcagcaaaat agcagactaa
     7981 ctgaccattg tccgaaatta ttctcaatcc ccgtatcagt tattcgctct tcggacgttt
     8041 atcgaattca cccgcaaaaa atattcgaat ttggcttcgg atttgcagga agtacaatga
     8101 ttggaattaa tgtcaaagaa atgcttgcag tatcattagc aagacttcat gagattgcga
     8161 acaataatcc agaacaacaa atatcgaccg aagcattccc aagtcttaat gaaatagctt
     8221 cattagcaaa aaatattgcc gagcaatata tgtctgatat cggccaatcc tttccccaag
     8281 cagtaagaat acagatgata atttttggtt tttgctggag aacacaaacc tacaaaatta
     8341 taaaactcaa taactctatt gagcacccag ccaacctcca gattgaagat attcaaaatt
     8401 tatcaacagg cagacctgtt ttattaggtg ataacttgca tgaacttaat gagtttatag
     8461 agaataccag gcaacaattt actcctaaca caataaattg gtggcgtacc ccgtttattg
     8521 ctttgaataa ttggattaac caagaaacag tcaatactat tggtggatac atccaaatgg
     8581 taaccgcgca cccacttggt actcgaatat catttttgac caatttgaat gaaaatatta
     8641 tcagaacaag ctatgcagga attaacacta ctgagtcatt tggaccaact attggtggtt
     8701 ttatcctaat gccaatggat gggatgtcac tcccagatga aaacggctgg gatgttggaa
     8761 atcaagttac ccgtactgct gcggatagag ttgctactaa tagataaacc tatctttcaa
     8821 agttgaataa ttctttgatg tccatgtcca acccttttcc taaaacactt atcacctcaa
     8881 gggttggatt ccttactccc cgctcaatac cgctcacata agtacgatcg agaccacact
     8941 tatccgcgaa tgcttcctga gacaggcccg actggagtct gagttttttg agatgtgcgc
     9001 caaactgcgc ctgaatactg gatgtatttt tcatccagat aaaatgaact tacgttgtct
     9061 atcagaccac ggactatgag tcacacttcg ttgattcggc gtccttttct gcgccatcgt
     9121 caaacataaa taaatggcta agatccatcc ccagaccgtc cgagattgca caaatgacaa
     9181 caagcgtggg gttccttact cctcgctcaa ttccactcat ataggtgcga tcgataccgc
     9241 atttgtcggc taaagcttcc tgggataggt ccatctcacg gcgtagagcc ctgattcttt
     9301 tacccagtac ctttttgatt gatcccgtct tttccatcta gccagcatca atcttcgttg
     9361 attatcagtc cacggactat gagtcacata aaaagcgtgc tagcatacat tcgtgactca
     9421 tagtcaacaa tagaggtaaa cggaatgact aaaaaagact ggcaccccgc cgatatcatt
     9481 gccgccttac acaagcgcgg caccagtctg gcgaaactct cgcgggaagc gggactggcc
     9541 tcgtcaacgc tggcgaatgc cctgaaccga ccgtggccga aaggtgagtg gctgattgcc
     9601 attgcgctgg gaaagcatcc gtcagaaatc tggccggaac gttacctcaa tacgggaaat
     9661 aaacgtgaac cccgtaaacc gcttcacctc atgacaacaa cagaaaactc cgggacataa
     9721 cgacccgcgc tgtgagtggc aggacgcatt aatcaacgac acctgacagg agagttgttt
     9781 atgcacaccg aaccacaccc ttgctccatc gctcttacaa ccggaggcca ccttcacccg
     9841 gcctcccgga ctgaaggctt tattcaccta ttcaggctac atgcctgctc acctacacca
     9901 ggccgtactc tcatgcccga caaacgggag aacgagccat gatgacctac tgcgcacgca
     9961 tcgttgctat cgggctgttt gtcgccgacg gactggcaga caggacgctt atcacctttg
    10021 acagcaacgg accgaaagac tgcctggact attccctgtc gctggaaccc tcattccgcg
    10081 aagacgacct gctgatcctg cccggcgatc ggctgctgct ggcgagccat gactacctgg
    10141 tgaccgccgt gggtaaagag gcacagcagt ctttgtttga acttggccac ctgaccatgg
    10201 tgtttaacgg cagcctgaat gcctgccatt cgggagccgt tcatctctcc ggcccaatcc
    10261 ctaaacttca cgacctgtac ggtgaactgc tgattgagga gggacggcca taagcgctgc
    10321 cttgaatcca tccacaacaa caaaagagaa gacattatgg aactcaacag aagtctgaaa
    10381 gcccgtcacc tgacgatgat ttccgtggga ggcgtgattg gtgccggatt tttcctcggc
    10441 gcaggcgcgg ccattgcccg taccgggccg tcggtggtgc tggcctacct tatcggcggt
    10501 gtcatcaccc tgttcattat gctgttgctt gccgaaatgg cggtcgccag gcccgtctcc
    10561 ggttcctttg aatcctatgc ccgcaccgcc tttggcccat ggtgtggctt tatcaccggg
    10621 tggacctact ggctggcctt cctgattggc ccggcgtcgg aggccatcgc tgcaggcacc
    10681 ttcctgaacc tgtggtttcc cggagtcccc gtctggctgt tttgcctggt gatcgcttcg
    10741 tcgctgactg ccatcaacat ggtcggcgtt cattttttcg gtgaagtgga gttctggctg
    10801 agcctggtga aagtcgtggc gctgctggcc tttatcgtcg cgggctgcta cagcctggga
    10861 ctggataacg cctcacccgc cagcatcagt catccgaccg acagcggcag cttttttgct
    10921 ggcgggctgc ccggatttct cggcgcgatg ttaatggtga ttttctcttt cggcgggaca
    10981 gaggccatcg gcaccgccgc gggtgaaagc gagaacccac agcgtgatat tcctcgtacg
    11041 ctgcgtggca ccgtggtgcg gatcctgttg ctgtatgtcg gctcattgac cgttctgctg
    11101 ctcgttctcc cctggcagca ggcaggtgtc agcagcagcc cgtatgtcga agcttccggc
    11161 attctcggtg gccatatggc gcggcatatc atgaactttg tggtgctgac cgccgcgctc
    11221 tcctgcatcg ataccggggt ttacgccacc tcgcggatgc tgcacgccat ggcgtctgac
    11281 ggctatttcc cggcctggtt tgcccgcctg cacccggcac ataaaacacc ggataacgcc
    11341 attcttgcca gcagcctggt gctgtttacc ggcgtcatca tcgccattct ctcgccagat
    11401 gcgtatgtca tcctcgccgg gatttccgga ttcggctttc tgttcacctg gctgatgatt
    11461 gccttaagcc agcccaccct gcaccgaata gccctgcacg acggaacgct gaaatacaag
    11521 gctccggccg cccgctacat ccagccactc accgtggggc tgattgcgct gatcatggcc
    11581 gggcaatttt tcaccgacgg cggcggcatt accctggtta ccgtcctgat ctggctggcc
    11641 ctcgccagcg cgtactacat ctttgtcgtc cgaaaaaaca ggaggctgtc cgatgaaaaa
    11701 agtcatgctc aatgaactga ctggccggga aatccgcgag ctgttgcagt ctggtaagga
    11761 actcactgct atcgtcaact tcggcagttg tgagaaccac gccgatcacc tgccactcgg
    11821 ccccgatatg tttgtacccg aaatcgtcgc ctgccgggtg gcgcagcggg tggataatac
    11881 ccttgtgctg ccctgcacgc cgttcggcac atccgttcac tacaatgcct atccactggc
    11941 ggtcacccta cgctacgaga ccaatatcgc gctggcggaa gatatcttca tcagcctgct
    12001 gcaccacggt attcgccgga tcctggtgat taacggccat gacggtaata ttccggcgat
    12061 tgaaatcgcc gcccgcaatg tgaaggcccg acatacgcag gcagcgattg tcgtggttcc
    12121 ggcgtggtgg gatctggtgg gacatcatct cgggaaggga tttttcgact catgggataa
    12181 caccggacta ggacacggcg gcgagggcga aacgtccgtc atgatggcgc tgattccgga
    12241 gctggtggaa ctgaaatatg cgcagaaaca gatcccggtg gaggtgttcg ctgattatgg
    12301 cgtgaaccgg atctgggata tcggggaaat cagcgccacc ggagcaaccg gcgatccgcg
    12361 ctgcgccacg gcggaaaaag gccagcggat cctcgcctgt ctggtggatg tgctttgcgc
    12421 gttgctgaga aagctggatg cagctaactg gcaatatgcg ttaaataaag acgcaaacat
    12481 gtaatcgata tgctcgggca acctgtggag tggcttttgc ctttccacag gttacccgga
    12541 agcgatatcc caaccgggta accgattggt gttagcccag aatctattat acttcaatga
    12601 gtaatattat tgctgagatg aatgacagtt caggtttagt caccttacca ccaacaaatt
    12661 ctgcacattt actcctgaga tttgctaatt catcttttaa tttttcgttg ctgtgttgct
    12721 taagatatat ttgtcctctt agctcaactt cagtacttgc cttcaaaaat tcctttcggc
    12781 tttgataggt gggatcgtta tcgagatttt ttggtatagt tattaaacat ttctgaaatg
    12841 cgattaaagc ttcgattagg agaagtctag agttttcttt gtattgaggc ttccactgat
    12901 gcaacgaccg ccaagcaagt agtaaaccta tcgttgtcac aacaaacgtc aaacaggcag
    12961 caatagcagc aacccccgcc cattttgcag cttgttgatt agccaccaat gtgctcaagc
    13021 ttatgaaatc cgcatctata tgcactattt ttacctcagt aagaaaagtg gatgtaaatc
    13081 tactttttca tttttgaaac agcaacttga ttttaagcca tcgtcctttg agcaagattt
    13141 tccgcaccag ttgaatcacg tcaaccacca gcacgccctc ctctctcagt gcatcccatc
    13201 ataatggcaa tctgtaaaac agcagtcctt gctttaaaaa tgtgtcattc ctcaattgtc
    13261 tggccaagct tattcaaatc atccagcccc gagatttgct tgagcacaga aaggtactcc
    13321 tgatggttat cataaagcca gttaagtacc tgaccgttcg ccagcagacg ttcaatgtga
    13381 tttttaatga taaccagccg aaggttattc tcagcatatt catcctgaag atgctgcgtt
    13441 tccacctgaa cggaagccat ttctttttcc agtctttcga gattttttct gatatctgtt
    13501 tctctgctgg catctttttt aggcgctaca agatgttcag gggcaacggc atgcagcatc
    13561 gacatcgcaa atttgcgggt gaaattatta atactgatca tggtgttaac catttctatc
    13621 tgcttataga actttagttt tttcaacaca gagaaaatgg tctgcggtac atttttttct
    13681 gccagtaacg ccatgacttc aggggcaatg ccatcagaaa ttttcagttt accaatcagc
    13741 gtatctggcg tgacacccag tacggcgcat agtttttcaa ctgaaacgcc agattcaacc
    13801 gctttaagca gcatgttttg ggcctgcaca atggtaaggc gactaacacg tttgttatat
    13861 gaatatgtat cctccacagg ggatatcaga caatgagcag tagttattcc gagttctttc
    13921 aaaacttcaa cgcgaagatg tccatccaga atgactttat cattggcgtc attgatataa
    13981 accacaatgg gttcaattaa cccaatttca tcgacagagg ctttaatttg tttgtacttc
    14041 gtactgaatt taacattaac cggcagcgtt ctggcataca ccagtgattc caccttaaag
    14101 gtataaaatc gggagtcaaa acattgtgaa atcatcttat ttctcccccg atacatcact
    14161 gccatccagt aaagaaggaa tatcgtggat attattactg tgcagcaatg taacaaaatc
    14221 attatcttta agtaaataac caaatatttg ctttactgcc agaattttct ctttaaccat
    14281 tgtggatttc agcagaatgg ctttttgctc attaatgttt tcctggtaga cattagccag
    14341 ttcttccggg gtcagttttt tctgtttttt cccaggaaaa aaagccgtac taatcgtacc
    14401 tttatcgcct ttcttacgtt gttcaagtat gcgcctgatc ttattaattt gcccaccctt
    14461 gatatgtcct tctttcaggg cgaccgtcag cagttcctga gtttcctcat ctcctgaccg
    14521 ggatatttcc acagcaagat acaagggaat atcgccagtc tcaaatgccc gcaaaagatg
    14581 cttctctccg gatgacaaca gagtgatcac cccatttacc caactcgaat tatacccaag
    14641 ccgcaagcct atttcacgat cggtcaggcc actggtcttc attcttctga tatcttccat
    14701 tagctctgcc gcccggggat tacgtcttgc gatgttttca gccaggctca tcaggtgcgc
    14761 tgtctcttca tcaacctgct tgatgattgc aggaattttc gtttcaccat actcaatata
    14821 cgcttcaagt cgtccctgac cacaaatcag ggcatattta tagttttcat tattcactgg
    14881 gcgcacgcta acaggccgtt tcagaccaat ggtttttatg ttctctttta tttcattatg
    14941 aatgaattta ttccttttcc gtggatttgc gatataaata tcactgatat cgacaagttc
    15001 tatctgttca tcaaaagatt tcattattta ctctccagac cagttcgttt tacaagattt
    15061 tccagacatg acaaatcatc aaatcgatat agttcgagga taaaaccatt gttctcatga
    15121 agcgttaaat cgttatacaa cacatcaata gccggaaaca taaagtaatc aaccacggag
    15181 ttatttgtgc tatccaaccg tggaaagatc atgacatcac aataatggga tacattaaat
    15241 tttatcttcc aacgaagctt acctgacgac aaacgttggc agcgaaccag attagcggaa
    15301 acaaaaaact catcattaat atataatgaa ccatcctgat gcatctctgc ccaaccgcca
    15361 tatcggtata tatcctgcac caaattatcc atgaaagtga gatggcattc tgcaatctgc
    15421 ttttttattt caagataaga gtaatcatgg cgcgggtgat aaccaataag ggtgtaagcc
    15481 ctgactaacc caccaaaccg tgaacgaaaa atgctgctcg atggaaacag ttcctcttca
    15541 tcaatcacaa tcccggataa tctgccacga agcttcagca gatggcgtag tttatccagc
    15601 agttcatcat cactgtactg aacggccctg gcttcaataa tccttttagc cgcctcaaat
    15661 ttctctactg ataccacagg gtcccacgcg ccgtcacatc tgatccactg atcctgagga
    15721 ttcctgacac ttcgggtctt caatcgtccg gaggttctgt tataaacatt attgccaata
    15781 tatttttcat tggtcagtat ctgatgaatt ttacatcggc tccactggag cccattctcg
    15841 gcggggatgt tcttccggtt cagttctgaa gcaataacaa actcaggctt gccctcatca
    15901 ataaacatgt catagatttt attgactatt accacctctt ccgcgggccc cgggatcaat
    15961 attactctgt cagtttgcag gcttttcctt tgccccggca gaagcatgcc tttcggatta
    16021 gtatgttcat caattaataa tctccgaatg ccatagccag gcataccacc ttgataataa
    16081 ccccgccgaa cgaggtttgc ctggccaaga aaaacttttt gtgaaaggtt tttactgtaa
    16141 gttgcggcgc catagcggga gtaagataat cccagcataa acaattcggt ctgttcttcc
    16201 gagaaaggct ccgcgcaata aataatgcgc acaccattac gcttcagtag ccacgagtaa
    16261 tgagcagcct cttcaatatc ctgaaaacga ccaaaccgac tgacatcgta aacaagaatt
    16321 aacgagactt ttattattcc gttttcaaca tcatataaca gcgaacgcag gccaggcctg
    16381 ttatccaggc tcagcccact tcggccagcg tcgtcatagg tatatttaat ggtaatacag
    16441 tgttcttttg catacttctc gatatattcc cgctggttct ccggcgaata tttctgatgc
    16501 tcggtagaca ttcttaaata ttgagcagca atcctgttcc cggcgacaat atccgacata
    16561 gaatattcca tatgattacg cgctatttat aactcatcgt aaacgtcgcc gtaccgtttg
    16621 ccgcacctgg cgtgatcgag cctgcagtct ggtaatatcg cgccttaagc tgcacgatct
    16681 cgtcaccata taccgactgc cagtaaaacc gttcctgacc gtactggaca gcggcatcat
    16741 ccgggcggta atgaagttgt atccccacgc cagacgcccc gccgctatca atcagcatga
    16801 cgccttgcga actggcggaa gcatccgcag tggcatcgat acgtacgttt attctcgccc
    16861 cgacatcgca gctcaaacca atatcaaagc tcacccagtc ggttgaagat cccgcaccac
    16921 tgaattcgct tttatcgtga tcccccatcg ggacatcaac agagggcgtg gtcacagtac
    16981 aagcttctga tttcactgtg ttcatcccgt tcaacgtgac gttggcaaaa taaaactgat
    17041 tcactaccga agcgcgcgcc agcacaccgt ccgttaatgt accgctggtg atatccccgg
    17101 ttttgataag ctcgcccttg atgccatcac cgggaatgga tacccaggtg ccgccagcgt
    17161 tgtaggcgta gctggtttca tagggcaatg ttttacctgt cgaggccgca ctcagacgta
    17221 atcctacccc tggcaggttg gtgttgtaga caccattccc atagccactc agcgtccccc
    17281 attgcgtcag ttcccagcgg gcggtccagg cctcccgctt acacccggca atgccgtcgc
    17341 cattgttgta tgcccctgta atggcagtgg caatcgtgct gccgacctgt gcatcccgct
    17401 ggataaccac gttgccgaaa ttcaggtaac ccgatacttc tgatgtcaca ccaccgtaga
    17461 acgaacaggt ggcctgaacc tccggcagga cggctatcat caacagcagg aagaaaagaa
    17521 tttttctcat ctcaacactc cggcgacgtc atttataggt cagcgtgaag gtcgccgtgg
    17581 cattggcaac acctgcagta atcgtggctg cggtctggta ataccgcgcc ttaagctgca
    17641 ctgtctcagt accgccattg ggagaggtat agtaatagcg tgactgacca aactgtacgg
    17701 cgctgttatc cggtgcaaag aacaactgga caccgatgcc cgtagccgcc atgtctccgg
    17761 gcgcgctgtc cagcttcatc acgccagcca cgttggacgg atcctgtacg gcatctatct
    17821 gaacgttgat acgggcactt ttatcgcagt ccagcccgat gttaaacgcc tgccagccgg
    17881 ttcccgttcc cggcccggag aactccgctt tttgatgctg ccccagcggc acattgacat
    17941 caggcgtggt cactgagcag gcgatggtgg tgatttgccc accggaaaat gtaatgggaa
    18001 tttcagtcga ccactgcccg ttctcccttg tccccgcgat aaaacgcccc aggttgccgc
    18061 tcagcgtacc gggcgtaatg tcgccggttt ttatcagccg gatccgcgcc tgatcgttaa
    18121 tggagggatg gtagccaacg ctgtggctta tctggagcgt gaatacctgc gactgcccgc
    18181 tggcgatacc gatccagttt tccggtaacg gattgctgtc gaccgaggag cggccttcaa
    18241 acacaaatcc cacacctgcg acattactgt taaacaccgc ctcaccattc caggtgacgc
    18301 tggcgcggga gcccgtgttt ttgatgcctg cattgatgac gtattgtggc gttgcatcat
    18361 aattgcagtc ctgataggcc atcccctggt tcgacaccag ccagtcgcta atgggttccc
    18421 ccaccacagc atcccgctgg accaccaccg taccggggct gatggagaaa ctcaccgaag
    18481 acgtgcaaaa cgcccacgac gcctggcaga acaggatccc cgccagcaga atcatcccgc
    18541 gccatactct cattttgcca cctccctgca ttcctggttc accaggatca ctgacggctg
    18601 gttttcctgt tccggtaaac ggtaatgcac cacgcactgt tgtgctccgt tccagttaac
    18661 cgcaatatcg ccttcctccg gcacgccagt cagatagacc tggccatcat cgccgacaat
    18721 accgctgccg ccctctttca gcttcgcctc tgcgccaaag gggactggca gcccacggta
    18781 cagcagcgtc atgagcacac gccgcccgac acgggtgtta aaatccgcca tcaccacggc
    18841 accctgcgtg ggggtcacgg tctgtacggc agtgtcggta tccacttcgt cgccaagcgt
    18901 gctggtgtcg agcgcaatac ggtttttctt gtacgggttc acatacggca ctaccgcata
    18961 accgcgccag tcggtgtaaa ccccggtgtt gttctgtacc ttcgtatcat ccgcaccggg
    19021 cgctttcacg atcgccagcg actggccgag cggctgcgaa agcgtgatgc catgcggatg
    19081 cgcgacaata ccgcccttca gcccgtagtt aacctgctgc gtgtcgtcgt catagttgta
    19141 gcctccggtg atttccccct gcccgccttt atagtcggcg ttgatattgc cggagccgcc
    19201 cacaccatgg ttggtgtagc tctgctggag gctgtagctc aggttgttgt cggcaagcgc
    19261 cgtaccgttc agccctacct gctggcgggt atcgccgtgt ttcgcggtgt tgacgctgta
    19321 gctcgcccag gcgttcggca taaagcggct gagcggcacc tggatgttga acgccagttg
    19381 ctggtcggca ggctgcgtgc tctccgggta gtcgctgtaa ctccaggtaa cgctgtagct
    19441 gataccgtcg atattcccgt tccagcccgc gctcaacgtg cgttcgtagc cgtcctcctg
    19501 ccagtaatcc tgctggtagc cggagataaa aaacgagcca tagctaccga tactctgact
    19561 gagatctaac tggaggcgct ggcgtttgtt gtacgtcatc tgccagccgt tatcatcatc
    19621 agccctcagg tcgttggctt cctggaaggt gtaaaatccg gcggtggagt agcgatatgc
    19681 cgccagcgtg aagctggtgt cggtggtctg aaaatccttc gaatactgca cccggtacga
    19741 ctgcccgttg tgggtttcat cgttattgag cgtggtatgg gcggcggtga tatcggctga
    19801 cagcgaaccg agttcaccga ggccaaaacc gagccctgcg gcgccagact gataatcgtc
    19861 cgagtacagc gtgccgccat agacggtgat agcatgcggc agaccgtaaa tcgcagtgac
    19921 ctggccgaac tgcggctcgt cgctgtcctc attgccgctg cggtatttcc cggcggtcag
    19981 ggcatatttc aggcggcctt cacgttgcat aatcggcacc gccgagaacg gctggacgaa
    20041 ggtgcgctcg ctgccgtcgg cctctttgat ggtcacttcc aggtcaccgc tgcccgaggt
    20101 cggatacaga tcggtgatgg taaacgcacc cggcgcgacg tagctctggt agatgatgta
    20161 gccgttctgc ttaatggtca cctgagcgtt agactgggcg ataccgcgga tcaccggggc
    20221 gaacccgcgc aggctgtccg gcagcatgtt gtcgtcagat gccaactgcg cgccgcgaaa
    20281 ctggacgctg tcgaagatgt ccgacggcgt gtagctgtcg ccgagcgtca gttgtccctt
    20341 caggcgctgg atatcccgtt gcaggtacgt gtttactgaa tcccagtggc taccattatc
    20401 ttcgtcataa ttccaggtcg agtagttgcg tagccgccac gcgccgaggt tgataccgct
    20461 ttgcaggttc gcgtagtagt tatcatccga accgttttgt ccatcgcgcc aggtgttgct
    20521 gccgctgagg ttgtagttca gcagtgcggc ggggacacct tcgtcccatt cggacggatc
    20581 cacgtatcct ctggcgctgg cattcattgc cgcctgggga atactgacgt tcaactgcaa
    20641 tttactgaag cgcagttcag tgttggccat cgggatatag cggccaatat catccagtgg
    20701 ttgatccggc ggcagtgctg ccagtgccgg gaagacgtcc gttttgacgc cccaggcttt
    20761 cagcatcgca ggcgttatct gcgggctgag tttctttttg gctccgtcaa taaaagcaat
    20821 atcctgtgcg tcgctctgct tttcaccgtt cacgaagacc gttacgcggt aggtaccagg
    20881 tagctgacca ccggattccg caaaggtgga taaatccgcc accggctggc cggggttatc
    20941 aatctccagc gcgtgggggt taaacacctc acgcgcctcg gcattacgca aatagccaga
    21001 caaaagcact attgccaaca acagcgtcag caggattatc atcagacgtg gcgtcgggtt
    21061 attcatcacg tcctccttca cagcttacgg ctttcggtct ggctgacacc gccatagtcg
    21121 ctgatggcct gccaggtcac cgcgcttgca gtcgctgacg gtagcgtgaa actcacactg
    21181 cctttcggcg gcaccatatc cgcctcttta atctcctgcc cgtcgacctt cagggtgaac
    21241 agcgtgacgt aatacggtgt gggattgctg acggtaagat ggttaccgct gtgggcaacg
    21301 ctcaactggt gataggcact ctccggctgc ccttccaggc ctgccggacg gtaaaacagt
    21361 ttcagccgcg acttgaccac tacctgcaac acgttggtgc gcgaatggct gtcgtcggta
    21421 gcggggatcg atttcacgtt cagccagaac accgactccc tgtcctgcgg caggttgcct
    21481 ccggtgcgca caatgcgcag catgttctct tcgccgggat taatgcgaaa caacggtggc
    21541 gtgacgataa acggcgtttt gcccccctgt tcgccactgt ctacccagct ttgcaccagg
    21601 tagacgccgg atttctccgg gttgcgtacc gaggcggccg tctctttttt atccccgtca
    21661 taaatcacgc gcgtaccgcc aatcacgatc ccggcctgcg cctgtacgct caggaaagcc
    21721 agcaacagtg ccagcaccac gtctgcatac ctgttcatct tcttgttctc cggacgaaaa
    21781 atacgcatcc ctgcgaaaca gtccctgtta tgcggcggta caccaggtcg ggtaggatgg
    21841 gagaggtggt gaaccccgcg acggcacagc gttatttgta gttaatggtg aactgcgagg
    21901 taccgttagc tgtacctgtg gttaccgcgg taccggtgga aacatagcgg gcgacgaagt
    21961 ccagcgtatt gctgcctgcc gcgagggggt aatccattga ggccgtatgc agaggaatga
    22021 ctgcaccatt tttatcggcg atttcgatac caacgccggt cgcaatcccg gtaccgctgt
    22081 ccagcgccag caggttctgg tttacgccat cagacgtccc gtcaaacttc acgttcgccc
    22141 cggtaacggt atcagggcaa ccactcaggt tgagcgtgaa gcgcgtcggg gaggctttca
    22201 tcccggccgc accgttcagt gcggtttgcc ctacattgcc cagatcgacg ttcaggtttt
    22261 gcgaaccgct gtccacggtg catgtctggt cggtaatact gccggtaaaa tggatggtgc
    22321 catcattggc gaccgcattc ccggccacca gtaaaaaaca cgcgcttaac atcgtttttt
    22381 tcatacgttt ctccttgatg aaagatcact cagggctcaa ccctgattca ctctggtgac
    22441 gagaaaaccc gcgtaagact ttcccgttag tgccagagtg gtgagttcag gagaaaatgt
    22501 ggtggacgaa gggggtgaaa aataaaatgt cagtggaagt gaagtatggc tttcaaaatg
    22561 gcataaaacc gtaatgcggt gagtgagtaa aaactggcaa cgtgaaccac ttaagcacgt
    22621 tgccagccag gaccagagag agactacata tttgttacat taaatattat tttaaaaaca
    22681 acaagatagt ccgttaccaa aaggggtctc agaaaaattt tgacagtttg ctaaattttt
    22741 ctgatactac tttacattct ggcactaatt gtgtggtttt ctctcacgtt tccccagaca
    22801 ttcataaacc tctgcgtatt tgtcgccgta taaatcaccg tatgccggat gttccggtgc
    22861 cccagataat cctgtatcag acgggtgtcg cgccccagat ctgccagcgc gtaaccgcaa
    22921 ccgtgtctca gcatatgcgg ataagcggaa atgctgacac ctgccagttc tccgtaacgt
    22981 ttgagtaaca atctaacctg atggcgcgac agcgcaccac ccttctgcga caggaacaac
    23041 cagtctgaat ccgcatcccg ccagcggttg cgtaccgcca gccagcgcat cagcgcaggc
    23101 agttcattat caaacagcgg atgctgcgtg gagagactgt ttttcaaccg ccggacatac
    23161 accgagcgcc cgatcagatc gatatcagac aggcgcatcc cgcacagttc actgacccgc
    23221 aggccgtgta aaaaacacat ctgaatgagg caataatcgc gctcaaaatg gatccctctg
    23281 tctgctgcaa gcaatatctg tcccacttca tgacgagtga gatgctttcg tttttcaggc
    23341 atcagccttg ctccttatca aaataaaaaa tgaaaaaaaa tcaggccaga actgccttca
    23401 cagtccaggc ctgaaccaga gaataccacc agaaacaacg tctggtgatg atgtaaaact
    23461 caacaatttt ctgtaacggc ttccagtttc ataccgttga tgaacagata ttgcggtaca
    23521 cccgacagaa gaaatatctg ctgtcggagg cgcgatgcga gcccaataca tccttctgct
    23581 gctgtgcgct gctttccccc tcagggcgat tactacgccg gagatcgcag cctctgcgct
    23641 tacgccggac tgtgtgaaat accgggtggt cggcctgtgc tactggctgt actgcacgcc
    23701 gttcggctgt acggtacgca cgtcggtgaa ggtcagccat ttccgcccgg atctggtggt
    23761 gtcggcttac agcaacaccg ggcagaatcc gtgggcggag atgtcgttac tcagcccacc
    23821 gttgcccggt gttgccgaag gcggcggcga tacgaatcct cgttctgcca gccagcacag
    23881 caaggtccgc tttaagaatg ccgatgccat cggcttcccg gcgggtgatg aactggcaaa
    23941 atttttcacc cagtttggct atatctgctc accttcatcg cagccttttc aaccgtactt
    24001 tctgagtcaa cccgatacgc tggcgtggcg cagcggtgtg ccggaaatga catacccgga
    24061 ggcgctgacg ccgggcatgc gggaagtcgg tcagaacggc gatatgtggg gcaatatcta
    24121 cccgcgagcc ggagccatca accagacgca cgactataaa gccgccgccg ttatcgccca
    24181 gcgcgcggcc gatctggtga cccgtaccgg gcaaccgcat ctctacacgc cgctgaccgc
    24241 ctcatcgcgc gccgggtact ggccgccctc cccggttatc gaaggcgaca gcgataacca
    24301 tcgctggcaa atgctcacgc cgaaaaaatc cgccgcctgt tcggtgttcc cggatggcag
    24361 tgccactgac acctacgcca acaaactggc ggaggatggc gcgtacgcct ggacgctgtg
    24421 gcggccctat aaatgctgtc cgcgccgggg gcaaaccttt ctcggcagta cgggttaacg
    24481 gaggcggtga tgagagccct tttcctggtc ttattgtttt tcgccggggt ttgcgtggcg
    24541 gcagacgact acagtttccg cacgcgaggc gcggttgacg acctgcttta ctaccagatt
    24601 ggcggcggtg aagcggtcat gccgcctccg gtacgccgta gcccctcgcc gcttagctta
    24661 ggtcttggct ggaacgccga tatgatgtgc ggcaatttcg acatcgacac caccgtgcgc
    24721 aaccagctca acggcatcac cgacggcttc cagcaactga tgggcaacgt tatccagagt
    24781 gccaccgggg cggtcgccag cctgcccgcg atgattatcc agcgcgccaa tccgcagctt
    24841 tatgatctgc tgaccaacgg cgtgctgcag gggcggctgg attttgataa gtctctgctg
    24901 aactgccaga aaatggcgga gaagatgacc gatttggcgg ccgattccgc ctggttttcc
    24961 ggagccaaag cggagaacta ccagtccctc gccgccagcg ataacgatgc catccgcacc
    25021 gaccagaaag cggcaaaaga ggccgccgaa aagggtaaac gctgggtggg cggtgaaaag
    25081 cgcggcggca aaggccagct gccgattaag gtagtccatg acgccaccgc cgcaggatgg
    25141 aatatcctca accagcagcc tgccaccagc actacttctc ttagctcctc tgaatgcgac
    25201 ggggaacttt gtagcacctg gacatcccct gaagaggccg ccgggtggat gacccgcgtc
    25261 gttggcgagc agaccatcag cgtggcgcag gtcaccgacg atccggatgc gcgttccggc
    25321 gcgcaggcgg ggatcggcct gcatccgctg atcgacgaag aaaaggcgaa gatcctgccc
    25381 ctcctgacag atctggttaa tggcagtcag aaacccggct tcgacaattt ggcgaaagct
    25441 tccggcggtt ctatgcaact gacccgcggc gtggtcgagg cgctgcgcga cgatccggac
    25501 gttgccgtcc tcaccgaacg gctggcggga gaactggcgc tggcccgcgt caccgagcag
    25561 gcgttgctgg cccggcgtac gctgctcgca ggtatgcgtg aacccaatat cgccaacgtc
    25621 aaagaggcgc aggagagcct gggcaaaacc accgcccaac tggatgaaga gctgaaccag
    25681 ttgaagctgg aactcgattt ccgccaggca ctgacgcgca ataccgccag ccagatcctg
    25741 caacgcaaac agcagcgcga ccagcttcag ggacaggtgg tggaggtgcc ggacgacagc
    25801 gacagccgtc tgcacaacct caataacccg cagccggaca gcccgcgatg accgccaaca
    25861 gctatttcga gtacgtgctc accctgctcg gctggctggt gaataacggc gtgtggcaga
    25921 cgctgaccgc caccgggctg ttcgccgtgc ccctgctggc aaaactggtt gccctgtggc
    25981 tgaaagcgcg cggccagggg gccgatgaag gcaacgtcgc ccgcctgctg gtcgtctggg
    26041 tggagcactc gctctacagc gcactgctgg tgatcttctt cgcgggtgtg ccgttcctca
    26101 atgtcgatat caacaccctc acctacgaca gccagcgcac aaagcagtgc ggctggtcgg
    26161 tggtacagcc cggcgattcc ggctacgcac ctttgattaa tgaactcagc ggccagagcg
    26221 cttccgttcc agtgtggtgg tacttcacgc atgcactggg caaaggcatc accagcgccg
    26281 ccgtcgccgt attgccctgc aagccggacc tgcgccagtt gcgctttgag gtgcagcaca
    26341 cccggctcgc cgatccggca ctggcgcagg agctacagga tttcgttaac gactgctacg
    26401 ggccgtcgcg ggcaaggctc aagcggctga gtagcgatat tagcgaggag caaagccggg
    26461 aagtggactg ggtgggatcg tctttttacc tcgccacgct aggctattac gaccgcgatc
    26521 actcccgttc gccccgcgcc gcctggcctt atgacgccag ccgcgacgac ggtctgagcg
    26581 atacgggtgg cggtggctat cctgcgtgta aggagtggtg gtccgatggc gatacggggc
    26641 tgaaaaaccg cctgctgaag caggtcgata ccacgctctg gcagcaactg caaaaactgg
    26701 gacagtctaa agaggagtat gaagaagcgg tactgcgctc gctggtcagc ccgcgcaata
    26761 tgcaggtatc gcagggcgga ctggtgtaca acgggtttgg tgggagtgcg ggggataacc
    26821 tcagccagac gcagttgctg agtcgtgtgg gcgggatcat cggcgcgggg ctttcgagct
    26881 acgccctgtt tccggcgttt gataacgtcc gccaggcgct gccgatggtg caggcgttcc
    26941 tcgaaatggc gctggtgatt tgtattccag taattctgct gttctcggca tgggatctta
    27001 aaacggtcat cacgctgagc tttgtgcagt tcgccctgtt ctttctcacc ttctggtggg
    27061 aactggcccg ctggctggat aactggctga tgcagatgat gtacgacagc gatacccact
    27121 catacttcaa cctctggggc ctgcaaaaca cctccgacga tctgattgta aatatcatta
    27181 tgggcgtgat gttcctggtg ttacccgcat tctggctggg ggcgctgacg tgggcgggca
    27241 tcaggatcgg cgcagcagtc gctgggggga tgggcagcgc ggtcggcgat atccgcagcg
    27301 ctggcgaaca ggtcgggaag atgatcgtca gtaaaacacg cattccctga agatttcgta
    27361 ctgacggtgt gatgaacagg gcttgtagct gagtttatgc gcaccgcata aactgtcgtg
    27421 tcagtgacaa tctcgtggta ggtagctgga gtctgaaaaa acgcaacggg ccgttaagcc
    27481 cgttgttcgt tacatcaccg cagttctatc actctcgccc gctcagcctg ctttcggtag
    27541 tggtcgtatc gctcccggaa ccatgcttgc tgttcatcac tcatattgcc ggtaacggca
    27601 tcgatatcaa ctggctggcc cagggcgtgc atacgggcaa aactgcgcgc cagaaaatca
    27661 aaattcttta atgcgtttat atcttgtcgg ttcattagca tatccctcca gtcattgtgt
    27721 ttactgttca aggtcatatg cttttctgat ttcagtatcc gcctgaaaaa gatcgttttt
    27781 tgagcgatgc gtgcgcatca atctacctgt gttcacattt caggaacgac aaaactcttc
    27841 agccccagta atgattttcc cctccctttt atcacccgcg cagaaaacac taccgccagc
    27901 catgcagtaa gttcgccatg tatccggaag gtgcgaacgg cgcagatgcg caggataccc
    27961 gtaccattcc atcccgcagg gaaacgccct gcgaagggtg ttccctgctc atttacagga
    28021 gaaacaccat gtcgcttttc gataaaaaac cgtcgttaat gccggtggtt atcgcccctg
    28081 atgtctggca atccgcagtg gaatttcccg ccgacccgca gcaggtggaa gaacggctgg
    28141 gcaatctgtt gctcgtcgta ctgctgaccc tgcgcaccgc cgggacatcc cgcaagaaag
    28201 tcacctttac gatctactgc cagccgccgc agggcgatat gaaagtccct gtcgcattac
    28261 cgctatcgct caactatcac ggccactatg tgcaggtctc gatggctgaa caggcataac
    28321 ctctccccgc cggagtcgct tccggatgga ggaggctccg gcttctttcc atgaggacca
    28381 cacgatggac agaagcaacg atatctacca gaaagtcacc gaccagatta tcgctgagct
    28441 ggaaaacggc aatgtgccgt ggctccgccc gtggcgcgat ggcgagccgc cgttcccgat
    28501 caatgcgtta tccggtcgtc cgtatcacgg catcaatgta ccgctgctgt ggaacagcgc
    28561 cgacaggcag agcttcgcca gcgaccgctg gctgacgttc catcaggtca gcgcgctcgg
    28621 cgggcaggtg cgccgggggg agaaatcctc tctggcggtg ttgtatctgc cgcgcacgca
    28681 ggaagagacc gacgcactcg gccagccgtt gctggataaa gacggcgagc cgaaggtgcg
    28741 gcatttcggg attattcgtg aattttggct gtttaacctg tcgcagtgcg acggcctgcc
    28801 gcaggcgctg tccgagccgt ttacccggcc cgcaacgcct gtggagatgg ctgaagccat
    28861 tacgatgaat agcggtgtga cgctgcgcca tcgccgccag tcgaaagcgt attacaaacc
    28921 cgcgaccgat ctggtgatga tgccgcaccc gcaacagttt gaaagcagcg aggcgtacta
    28981 cgccacgctg ctgcatgaac tgacgcacgc taccggacac gcttcacgcc ttaaccgccc
    29041 cggtattgcc ggagcggaac gcaccgaagc actctacgct gctgaagagt tagtggagga
    29101 gatgggtagc gccttcctgt gcgcccattg cggcattcag ggcaggctcc agcacgccag
    29161 ctatatcggc tactggctgg atatcctgcg ccaggacaag cgggcggtga tccgcgccag
    29221 cggtatggcg cgccacgcca gtgaatggct gctcaacgct gtaccacaac cctgtcagct
    29281 tagcgcctga cagcccaccc gcacggggag ccttccccgc cgggaagttg ctcccctttt
    29341 tctgaggatc aatgtatggg actcgatctc ttctttttcg aacggaaaaa aaacgcgccc
    29401 gatgaaagcc agtacgccag agtgggttat ttccgcaagg tgaatccgct gcttaaatgg
    29461 gtggataaac acgttgggct ggtgcgcaac caagtgctgt tagaagtcac caaagcaaat
    29521 ttgcaggcgc ttctgagcga cctgcagagc ctgacgccgg ataactgcgg cgcggtttat
    29581 ccgacgattg acggcttttt ctatggttat accgattatg gagaacacta ctggaaagac
    29641 gttcgcaccg tcactctctg ggtgaaggag acgctggaga cgtttgattt tgagcgagac
    29701 aggctgatgt tttatgcctg gtggtagtat taaagtggcc gacaaaacgt cggtcacttt
    29761 gcctcgtcgg ccagaaatac agcgccagat atacacatga gagcataccg tttttactaa
    29821 taaaaatgta aaaggcccaa agggagacac tgcaaaggaa gggcgacaag ggcaaagggg
    29881 cctgcccgga aaggggaaat atgctgaagt ggctaaaaag accatcccag agccagccac
    29941 ctacgccaga cgctacgctg ccggagggct ggtttgcgcc acaatccgca gaacaattgc
    30001 tggcgacgcc gttacgccag tcctgtttgc accagttacg gcaactgacc ccgctgccgg
    30061 ataccctgtt tgatagctgg atcctcgcgg cgattcatcg cttcgccgcg ttggtacaac
    30121 tgcttcccgc ctcacaaagt catcaccatt ccactcccgg cgggttgctg gatcattcgc
    30181 tggaggttgc ctgcaacgcc atccgactgc gccagggtta tctgctacca ccggaagcgg
    30241 gcgcggaaga acaggcccgg caggccagcg catggacggt cgcagtggtc tgcgtggcat
    30301 tactgcacga tacgggaaaa atcgccgtgg atatggagat tcatcaacgt gatggcaccc
    30361 gctggcattc gtggcagggt tatctggcac agccttatcg ctggcagtac cagagccacg
    30421 gtcgcgacta tcatctgcat ccggcggcta ccgcaatgct gattaatcag ttgataccct
    30481 cgccattact ggactggctg gccggatacc cggcggcttt tgcgtccctg atttaccagc
    30541 tcaccggaca ctacgaacgg tctggcgcac tggctgaact ggtgcagcag gccgataaag
    30601 cctctgtcgc ccgatacctc ggcggcgata tcaaaaccgc gctggcgcaa aagccctctt
    30661 ccttccccca gcaacttctt acagccttgc ggtcgctggt acagaacgag taccggctca
    30721 ataatcccga atgcggatcc gatggctggc tgacggagga tgcgctgtgg cttatcagca
    30781 aaaccaccgc cgaccgggtt cgcgcctggc tgctccagca tggagtttcc agcgtgccgg
    30841 aaaacaataa ccgcctgttt gacgaactgc tgacgcatca gcttattgtt gccaacgaag
    30901 ataaagcgat ttggcgctgc attatccgat cggataaagg ctggtcgccc gttgaaccgc
    30961 tgacgctact gcgcctgacg ccgtctgtaa tctgggggca ggatgatcaa cgtccggcct
    31021 gtttcgatgg tgatgtaacg ccggtaattg aagccgaacc cgctcctgaa cttagcgcac
    31081 cgactcaacc gttaccggag atagcagacg atccgaggct tggtgacaat aacaatttcc
    31141 tgcagtggct gaagaaccac actagtcctt cagccacggt gaatacccgg aacggcaagg
    31201 tacatgtggt ggaaaaccat cttttcttga tttcgccggg gatctttaaa acatacgcag
    31261 cagaaaccac aggcgatagc ggcgacggct ggaaactggc gcaacgaatg tttcaggagt
    31321 cagggctggc cctgcgctgt aatgatgaca gttttatctg gatctgcgag gttcgggttc
    31381 cacaaaagaa aagtagtcag ttgaaagggt ttctgatgga agatccgaag caggtgttgg
    31441 gggagaaagt gccggtgaat aatccgtggc tgaagttgct gcgcgaatag ctatttatat
    31501 caaaatactc tcttccaccg agctacagcg aatgggcttt aatacctaaa gagccgcatg
    31561 atattcaata attgatttca attcttcagg cgaaaaaggc ggattacgcc gatgcaacat
    31621 ggcgtggcaa tttgggcaga cgggaataaa atcttttttg ggatccagag agtaattctc
    31681 tttgatgtct gatagcggaa tcaggtggtg tatatggata aaacctttcg ctatctcgcc
    31741 atacaccttc atcatatcaa taccacatac tttgcacaga caaccatgcc aggctaatgc
    31801 ggcctgtcgg gccttacggt cgcgttcata gcgattaacc gttacctgcg tagctgcacc
    31861 ttcagtaaat acgctctgcg gtggcagttc atcaggaaaa atactgatgg cgggctgctc
    31921 gttacccaaa tgtttctcaa cgatctggaa cccttgatcg gtaagcaact tgacgcattt
    31981 cgacttgata ccaccactaa aatcttcagg ggaaaactca acgccagtca tatttgaagc
    32041 agcaataccg actatagctt tcggtggata actgcgccct tcaaagacca catcatacag
    32101 ccgggactcg ctgaactggt ggcgtacacc ttcatcatag gcacgcatag ccgcaataat
    32161 gcattccctg gaaagctggt ctggcagttt cttcttcatt tacaactcat catccctgaa
    32221 acgacatttg ctaactcttt attttatagc atattaattt atccggtgat aaacagcact
    32281 cttcaatttc gcgttaccgg gataggggca tcagtgcagc ggcaacgtaa cgtagcggca
    32341 gagaaatcgc gcctcattct ggttgtccag acgtacctga aagatatcgt gtgtatcctg
    32401 cgcggatgca tcctcacgcc acgccagcag accccgttgg ttcatgcgga taataatctg
    32461 atcgggcgtt tcgccctgat tcaatgcttc gactacctgt tgtaggggaa gcggtagcgt
    32521 ctgagcaggc cacatcactt caacacttac gagctggcgg tgtgtccgag gtgacatcgg
    32581 tggtttcctc catggctgga atttagcggt taattagcca tgaaataggg gcatttttta
    32641 ctgtttcaac tactgccgag atggctacgg aatacgcgct gactgatgca aaaatagctt
    32701 ctcttatgtg ctctggaaat ccagatggta cgctagtgaa aatggtggtt ccgtccggat
    32761 agcgccatgg cctttttgtt catatttcca cagatcacca ctcctgctcc cagccatcag
    32821 aagagttttt ttacccctcc agcttacgac ctgaagctta aggtccagag cggcaagcag
    32881 cttaaagagc gtatccaggc tggtcccctc aggatggttc tcaaactctg aaaccgttga
    32941 ctgtttcatg cccatacttg ctgcagtttc acgctgggtc aggttacgtt gcttgcgtgc
    33001 atccctgatt gcctgagaca acattttggg tgaggttacg ttcattttat cccccaggat
    33061 agatattttg agcatatccc ctatagggga taaaaattca tattctcagc ggagaaaaga
    33121 ttaataacta aatgaattaa aattactttt catggaaatt aaggggggac gatattcctc
    33181 gagccagtaa atatgaacat tgccagatgg cagaacgcgc cattttacaa gcattttcca
    33241 taacagatat aagactgatt gagcggttgg attaagcatg acgatatgtt taaaaaaacc
    33301 agttttatcg acataacgcc aaaatatgtt tacagcatcg acatatcaac gccatatgta
    33361 cattttttgg cattttttaa acatatcatc agcaggcttt attttttgct atcgaaatct
    33421 acagtccctc gtttcaatac aaaaattcca gcgtgtcagc taccttttct gggtttcttt
    33481 attggtttta gcagtgcagc aagagatgca gatgtcacga cttctgtttt aacttcagaa
    33541 tcgattgatt tttcttgtga tgtcgagaat tgcttgtcga aggcatcgtt ttttatcacg
    33601 tttccggtat gcctgtttgt gtgagagata gcagcaggac gagaaacacg cgttttattt
    33661 attagccggg aatcctgaag ttggcgtccc tgttcatcct cagcggcaag tttgttcaga
    33721 tccttttcaa gacccaaaat ctgcataact gacaaataag cgccgattgt cacgcccgtc
    33781 ccgcctgatt caagagaacg caaggtcatt ggcgacatcc cggctcgctc agcaacccgc
    33841 ttcgctgtga gcttacgtcg cagtcgcgcc agcttaagcc gttcgccaaa ctcagcaagc
    33901 aactgattag taccgggaac taagggagcc gtttttttag ccatagtgat aatattttat
    33961 cttttcaata cctatatagc cataatatta gcgcatcatg ccgaaggtgc ggtgcgccgt
    34021 ggaaggtgga acatcaagtt tacctgcgtt ctccactatc tttgatggga accagcaagc
    34081 ctttgacctt cagtttgatg atgtcgcgac tgactctggg ctggcattat tgataacata
    34141 agcgcatctt aatcgggcag ttggcgttac gccctgccat tgttcacaat aataccgttc
    34201 atgattcatg aacaaaactc tgttgtgaac atttcatcta aaactacatc ctcaaccatc
    34261 acttgccata ccatcccaga aatcattgat gatctgctca atcccagcat cacccacgtc
    34321 atgttgtcgt actatcacat cgatcaggcg ttctttatca agatggagta aatcctgcaa
    34381 ggatgagtgt tcactaattg tcgctatatc gcttcccaac agaaaagcgc ccatacgtat
    34441 gggatcccag ttcaggcgtt ccccttcgac gggcgattca tagatgcgtt cattctcgat
    34501 aacaccatat tcacagataa tcttaaagag atcggtcgcg tctttagtac tcccatgccc
    34561 acgatcacgc catgcaaaaa gttttaacag agccagtcct ggcaaggagc agaacggaat
    34621 attttcgctg ttatccagcc gcacaactac ggtatgagaa aagacttcgg caaatccatc
    34681 cacagccatt atgatctcac gttctggtgg ccaggcaatt tgatttcctt cagcaacacc
    34741 accaaaagga ataatatcta gctcgatccc tttgacaatc atacgatggg cattaccttg
    34801 gacaacttct gccccttcag caaggaaagc atttttaagc gcatcaaatc tcggccagct
    34861 atccacaaag acggcaatat caatatcacg ggttcggcga ccaatacttc tgctgtgtat
    34921 atgatggagc agaatttcac gagccgttgc acctgcaata aaaaacggga tcccctgagc
    34981 ggtgcaaata cggttgatct gactcagtaa cgtttcagta gctgaagaga tcggattcat
    35041 taaggtgaag atatttgtca ttgattatcc ttgctgtttc ccagtttctg tcatccccgc
    35101 ttgccagcag ttcagccacg catagcatcg cctcggcttt gtgattcagt acaaagttcc
    35161 cccaaaaacc ggcgatcagc tgtagtttcc cccccggaac aggttttagt cccagttcct
    35221 ttcgcctttg taacaacgga tgggatgtaa aaatcacgcc attttctggt atcaggtatc
    35281 ctccgctcag ctttgccgcc gcaatctcgc cgctccagta ttccccggac gcaagcgtca
    35341 actcatccca tgaaacaggc gcggataaag aaagcgaatc cagcttaggg cgcaaagctg
    35401 tagcataatc tttcagccac aaagcctcta actcgccttc attgattaag cgacgcccct
    35461 tcttcgattt gcggaaatag cgttgcgctt cgagataatc aaaagccttg ctgaccattc
    35521 ccagagagat acctgccttt ccggccagac tgcggtaagt ctcgttcagg gtctctgggc
    35581 tagacagcaa aacaaaaaga agcttcatca ccccttcagc aaaacgactg ctggcggaaa
    35641 cgggtttctt ttcatagcgg ccttcgatcc gcacatacaa gcctggaacc tggatacggg
    35701 cattccctgc cgtatcgata aagttgatct ggctgtcggc gcaatattcc gccagtgcag
    35761 acgtcaggcg gttacacacc agcagcacag gaaaatccgc aggaaagcga accatttgtt
    35821 cgcgaacagc catcagtgac tctttacggt ggataaattt tacttccaga gcaaatatca
    35881 ctgactcacc atccggccct gttagcttac cccaggcatc gtgcttacca tgtctaccgc
    35941 tatgttcata ctgcaaatgg aatccttttg gcagattctc tatcgcatca gacaaaagct
    36001 gttcttcctt catttgttca cacctcataa ctgttcatga atcatgaaca aaacaatgtt
    36061 atgaacaaat aaagaaagaa gcaacgtaaa tgatcaaaaa aagaccgcca gaaatgcgat
    36121 gccttattca accttccaaa cgtcattttc cattaaagaa agaagggtaa agagtagttg
    36181 gacgcattct ttcagggttg agtaaccatt cctactcccc cgactccacc tcctcctgcg
    36241 ccgccatcag aaccaactct gacttcgaaa tcagcaggtc tttcggcctc ccacctctca
    36301 acctgcgatt agccgagcca aaccagatag ccagccccca cggtgtttta cgctccttaa
    36361 acagtgaaag aatcaaacgg acaaccttga gaggccgcat tttctcatcc agaacataat
    36421 cagggtataa atcctcacca tcgaccttca gcgagaatat cttcccggcg gctttccagc
    36481 ctttcggacc agcatcagga tcggcggaac gaaagcccgc ttttgcggat aattcagacg
    36541 ccgtcagcca ttccgagtca gccagtattc tggctttcag ctcctctagc tgccggtcat
    36601 tgcgctcctg gaaactcata tggtcgagtg cgcctcccgg cgactttttg aagccatacg
    36661 gcacaacatg cctgaaccgg tttgcatcca gataatccgc ccaccactgc atcatctccc
    36721 aacgttcttc gagatgctgc gccttgtgga tatacgccgc acgcacgcgc ttgcgctcct
    36781 gatggctcat ctggcgctct accgcgtcgc ttgaccacag ccccgactcc accagcgcgc
    36841 tacaggccat agtgcgaaaa ccgtggccac agacatcttt ctgggtgtcg tagcccatca
    36901 cccgcagcgc tttgttgatg gtgttttcac tcatcggctt gcggtaattg tgatcgccgg
    36961 ggaacaccag ttcagttccc ggtcggcaat gctgccgcac ctcgtgcagc agttcgatgg
    37021 cctgacgtga caatgggacc aggtgtgggg agtgcatctt cgccccacgc gctgaatatt
    37081 taacgccggg gatcggctca cgctcagcgg ggatggtcca catggcgtta cgcagatcaa
    37141 tttcatccca gcgggcaaag cgcagttcgc tggagcggat aaacagcagc agtgccagtt
    37201 ttaccgccag ttgagtcagt tgacggcctt tgtaatcgtc gattctttcc aggaaatcag
    37261 ggataagatt cagatccaac gccgggtgat gccgtacttt gggcgtggag accgcgccgg
    37321 ttaaatctga tgcagggtta tgatcgataa tgccgttctg gacggcgtag cgcatcacgc
    37381 aggcagtgcg ctgctgaagc cgggaagcaa catccagttt gcccgctttc tccacttcct
    37441 tgatgggcac cagcagatct ttgaccttca ttttggtgat gtcacagctg ccgttcgtgg
    37501 ggaatacgta aagctcgaaa taacgcagca cccgcttctt atgttccgcc gaccagttca
    37561 cgttactggt gacccattca cgtgccacag actcaaacgt atgttccggc gttatcgcaa
    37621 ggcgttctgc ttttcggcgt gcactcggat cgatgccgga aatcagcaat aattgtgcct
    37681 cttcacgcat gcgtcttgcc tgtgcaagcg tcaaaagcgg ataagggcct aaagcgagct
    37741 ttttctcttt gtttacaaag cggtacttga tgtaccagcg ttttgaaccg ttagggttaa
    37801 ccagcaggta aagtccatgc gagtcactga gcttatacgg cttgccacaa ggcttggcgt
    37861 ggactatagc gcgatctgtt agtgccattt gggggcctca ctattatcga actacgtcta
    37921 agcccccaat ttggccccca aatcgcgtgg atgtcaatgg atgaaaaatg accaacacgg
    37981 aaagaccaca aaataaatat ggttattgtg gaaaaataaa ttacctttga attacaaacg
    38041 gatatggacg aatttggacg gaaatggaca caaaaaaagc cacccttagg tagctaatta
    38101 ttgcatcact tggtgccgaa ggccggactc gaaccggcac gtatttctac ggttgatttt
    38161 gaatcaactg cgtctaccga tttcgccact tcggcactga agaggtatgc ggaaaacgtt
    38221 gtggattata cctgtcgcac gccaccatgc aagcgccagc ttgctcgcac ctcgctaagt
    38281 gctgaaattt tcagcattgc ataccgcgat gtgtaacctt tgtcacactc caggcgcccc
    38341 gccctgccat gctctacact tcccagacca caccagagaa ggataaaaac atgtcgatga
    38401 taaaaagtta tgccgcaaaa gaggctggcg gcgaactgga agtttatgag tacgatcccg
    38461 gtgaactgaa gccacaagat gttgaagttc aggtggatta ctgcgggatc tgccattccg
    38521 atctgtcgat gatcgacaac gaatggggat tttcacagta tccgctggtt gccgggcatg
    38581 aggtgattgg gcgcgtggtt gcattgggta gcgccgcgca ggataaaggt ttgcaggtcg
    38641 gtcagcgtgt cgggattggc tggacggcgc gtagctgtgg tcactgcgac gcctgtatta
    38701 gcggtaatca gatcaactgc gagcaaggtg cggtgccgac gattttgaat cgcggcggtt
    38761 ttgccgagaa gttgcgtgcg gactggcaat gggtaatccc actgccagaa aatatcgaca
    38821 ttgaatctgc tgggccgctg ttgtgcggcg gtatcacggt ctttaaacca ctgttgatgc
    38881 accatatcac tgccaccagc cgcgtcggcg tgattggtat tggcgggctg gggcatatcg
    38941 ctataaaact tctgcacgca atgggatgcg aggtgacagc ctttagttct aatccggcga
    39001 aagagcagga agtactggcg atgggtgccg ataaagtggt gaatagccgc gatccgcagg
    39061 cactcaaagc actggcgggg cagtttgatc tcattatcaa caccgtcaac gtcagcctcg
    39121 actggcagcc ttattttgag gcgctgacgt atggcggtaa tttccatacg gtcggtgcgg
    39181 ttctcacgcc gctgtctgtt ccggccttta cgttaattgc gggcgaccgc agcgtctctg
    39241 gttctgctac cggcacgcct tatgagctgc gtaagctgat gcgctttgcc gcccgcagca
    39301 aggttgcgcc gactaccgaa ctgttcccga tgtcgaaaat taacgacgct atccagcatg
    39361 tacgcgatgg taaagcgcgc taccgcgtgg ttctgaaagc cgacttctga tccctttgcc
    39421 gggtgcctgt cgcccggcgt ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt
    39481 agcggtgaag agtgcgcagc cgttgcctat actccaggca ggaaactgga ggatatctca
    39541 tgagcgaacc cctgttaatt gcccgcacgt cggacacaga actgttttta ttgccgggaa
    39601 tggcgaatcg tcacgggctg attactggcg caacggggac gggtaaaacc gttacgctgc
    39661 aaaaactggc agagtctcta tctgaaatcg gcgtgccggt gtttatggcc gatgtgaaag
    39721 gtgatttaac gggagtagcg gaggaaggta cttcttcgga aaaactgctc gcaaggctta
    39781 aaaatatcgg cgtaaatgac tggcaacctc atgctaatcc ggtggtggtg tgggatatct
    39841 ttggcgagaa aggccacccg gtgcgggcga cggtttcgga tctggggccg ctgttgctgg
    39901 cgcgactgtt gaatctcaac gatgtgcaat ctggcgtgct gaatattatc ttccgcattg
    39961 cggacgatca gggattgttg ctgctcgact ttaaagattt gcgggcgatt acccagtaca
    40021 tcggcgataa cgccaaatcc ttccagaatc agtacggaaa tatcagtagc gcatcggttg
    40081 gtgccatcca gcgcggatta ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg
    40141 agccgatgct ggatatcaaa gactggatgc gcaccgatgc caacggtaaa ggcgttatca
    40201 atatcctcag cgccgagaaa ctttatcaga tgccgaaact gtacgccgcc agcctgccgt
    40261 ggatgctttc agagttatat gaacaattgc ctgaagcggg cgatctggag aaaccaaaac
    40321 tggtgttttt cttcgacgaa gcacatctgc tgtttaacga tgcgcctcag gtactgctgg
    40381 ataagattga gcaggtgata aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt
    40441 cgcaaaaccc gtctgatatt ccggataatg tgctcgggca gctcggtaat cgtgttcaac
    40501 acgctttgcg ggcttttacg cccaaagatc agaaagcagt aaaagctgcg gcgcaaacca
    40561 tgcgggccaa tccggcattt gataccgaaa aggcaatcca ggaactgggg accggcgagg
    40621 cgttaatctc gtttctcgat gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga
    40681 tcgcaccttg ttcgcgaatg gggccggtga cggaagatga gcgtaatggc ctgattaatc
    40741 actccccggt gtatggcaaa tatgaagatg aggtggaccg cgagtccgcc tatgagatgc
    40801 tgcaaaaagg ctttcaggcc agtaccgagc agcaaaataa tccccccgtg aaagggaaag
    40861 aggtggcggt ggatgacggt attcttggtg gattgaagga tattttgttt ggcactaccg
    40921 gaccacgcgg cgggaagaaa gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc
    40981 aggtgacgaa tcagattgta cgcgggatgt tggggagttt gctggggggg agaaaaagat
    41041 aagtcagacg tgatctgtgc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg
    41101 ttcgaactcg tatttgactt tgtcaacaat aataaaggga gctttcgctc ccttgttaga
    41161 ttacgatttt ctcattaaca gccacaggct gattaagaag aagctggcgc ttggcaacag
    41221 tgcgccgatg atcggcggga tgccataaac caacgtcagc gggccgaaga tctggtccag
    41281 tacgtagaag acaaaaccga agctgatacc ggtgaccaca cgcacgccca tcggtacgct
    41341 acgcagtggg ccaaagatga acgacagcgc catcagcatc atcaccgcca cggatagcgg
    41401 ctggaagatt ttgctccaca tgttgagctg ataacgtccg gcatcctgac cgctcgactt
    41461 cagatacttc acgtagttgt gcaaaccact gatggagagt gcatccgggt ccagcgccac
    41521 cacgcccagt ttgtctggcg tgaggttggt tttccaggtg ccgctcaccg tctgcgaacc
    41581 ggtaatctgt ttcggattgg tcaggtcaga ttcatcaacc tgcgacagac gccagacttt
    41641 atgttccggg tcaaacttcg cggtagcggc ataacgtacg gattgcagac gacgattctc
    41701 gttaaaggca taaatactga tgccacctaa ctcctcgtca cctttaaccc gctcaatgta
    41761 gacgaagttg ttgccatctt tcgcccataa gccttgctgg gtagagagca acgagccgcc
    41821 gtacatcgcc tgcgcacggt agttacgcgc catctgctcg ccctgcggcg cgacccattc
    41881 gccaatcgcc atcgtcagca agaccagtgg aatggcggtt ttcatcaccg acagcgctac
    41941 ctgcatacgg gtaaaaccag aagcctgcat caccaccagt tcgctgcgct gcgccagcat
    42001 cccaagacca agcaacgccc caagcagagc cgccatcggg aagaagattt gcacatcttt
    42061 cggcacgctc agcaaggtat acattcctgc gcctaacgcg tcgtaactcc cctgcccggc
    42121 ttttttcagc tgatcgacaa acttgataat gcccgacagc gacaccagca tgaacagcgt
    42181 catcatgatg gtggtgaaaa tagttttacc gatatagcgg tcaagtacgc caaaaggttg
    42241 catcacaccg ctcctttacg cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc
    42301 caaaggttga gaacaatcgc caaagccaga taaatcaggt taacggtcca catccacagc
    42361 gtcgggtcca gcttaccttt accgccgttc gatttcaggg aggtctggat caggaagaaa
    42421 agtagataca gcagcatggc tggcagcatc gacagtacgc gtccctgacg cgggttaacc
    42481 acgctcagcg gtacgaccat aagtgccatc ataaacacgg tgaataccaa cgtgatacgc
    42541 cagttcagtt ctgcacgggc acgatcggtg tcagtgttcc acaatgtgcg catgtccatc
    42601 tggtcggtat cgttcgggtc gagcgccacc gcctggtgac caatgatcgc ctgataatcc
    42661 tggaagtccg taatgcggaa atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg
    42721 ttgagagtga cgacctggga gccgtcgcgc agctgggtta aatgtccgga atcggccacc
    42781 accacagaag gacgtgcatt accttttggt cgaatttgcg cgaggaacac atctttgaaa
    42841 tcgctgccgt caacgctttc gatgaacagc accgagctgc cattagtcgc ttgctggaat
    42901 tgcccttgcg ccagcgccgc catgccaggg ttcgctttcg cttctgctaa cacttcatcc
    42961 tgatgacgcg atgaccacgg tcccgcccac atcacgttaa ccgccgcgac gattgccgtg
    43021 aataccgcaa ggatcattgc cgctttcacc agaaccgctt tgctcaggcc gcaggcatgc
    43081 attaccgtaa tttcactttc ggtatacagt ttgcccagcg tcatcagcag cccgaggaac
    43141 aggcttaatg gcaggataag ctgcgccatt tccggcacgc ccaacccgag aagggagagc
    43201 accagattcg ccggaatatc gccgtcaacc gctgcgccga ggatcctcac taacttttga
    43261 cagaagaaga tcaaaagcaa gatgaagagt atcgccagct ggcttttgag cgtctcccgc
    43321 accagatatc ttatgattat cactttaaat acgcccgtaa aaactcgtct tttgcaggat
    43381 tttagcttgt ttcatggctt aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta
    43441 agataattat actcaacgga ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa
    43501 taacgttaag attaacacga agtcatcgca acagcggaca tgagttacga aagcttgcaa
    43561 ttctatctgt agccaccgcc gttgtcttta agattcagga gcgtagtgca tggagtttag
    43621 tgtaaaaagc ggtagcccgg agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga
    43681 accacgtcgc ctttctccga ttgcagaaca gctcgataaa atcagcgatg ggtacatcag
    43741 cgccctgcta cgtcggggcg aactggaagg aaaaccgggg cagacactgt tgctgcacca
    43801 tgttccgaat gtactttccg agcgaattct ccttattggt tgcggcaaag aacgtgagct
    43861 ggatgagcgt cagtacaagc aggttattca gaaaaccatt aatacgctga atgatactgg
    43921 ctcgatggaa gcggtctgct ttctgactga actgcacgtt aaaggccgta acaactactg
    43981 gaaagtgcgt caggctgtcg agacagcaaa agagacgctc tacagtttcg atcagttgaa
    44041 aacgaataag agcgaaccgc gtcgcccgct gcgtaagatg gtgttcaacg tgccgacccg
    44101 ccgtgaactg accagcggtg agcgcgcgat ccagcacggt ctggcgattg ccgccgggat
    44161 aaaagcagcg aaagatctcg gtaatatgcc gccgaatatc tgtaacgccg cttatctcgc
    44221 ctctcaggcg cgccagctgg ctgacagcta cagcaagaat gtcatcaccc gcgttatcgg
    44281 cgaacagcag atgaaagagc tgggaatgca ctcttatctg gcggtcggtc agggttcaca
    44341 aaacgaatcg ctgatgtcgg tgattgagta cagaggcaac gcgtcggaag atgcacgccc
    44401 aatcgtgctg gtgggtaaag gtttaacctt cgactccggc ggtatctcca tcaagccttc
    44461 agaaggcatg gatgagatga agtacgatat gtgcggcgcg gcggcggttt acggcgtgat
    44521 gcgtatggtc gcggagctgc aactgccgat taacgttatt ggcgtgctgg caggctgtga
    44581 aaacatgcct ggcgggcgtg cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca
    44641 aaccgttgaa gtgctgaaca ccgatgccga aggccgcctg gtactgtgcg acgtgttaac
    44701 ttacgttgaa cgttttgagc cggaagcagt gattgacgtg gcgacgctga ccggtgcctg
    44761 cgtgatcgcg ctgggtcatc atattactgg tctgatggcg aaccataatc cgctggccca
    44821 tgaactgatt gccgcgtctg aacaatccgg tgaccgcgca tggcgcttac cgctgggtga
    44881 cgagtatcag gaacaactgg agtccaattt tgccgatatg gcgaacattg gcggtcgtcc
    44941 tggtggggcg attaccgcag gttgcttcct gtcacgcttt acccgtaagt ataactgggc
    45001 tcacctggat attgcaggaa ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg
    45061 tccggtagcg ttgctggcac agttcctgtt aaaccgcgct gggtttaacg gcgaagagta
    45121 agtttgcgtt ttaccctcac cctaaccctc tccctaaggg agagggaacc gatcgagcgc
    45181 aatagaaatt cattgctcat ctttcagggt gaggaatgca gcaatattta aatccaccac
    45241 aagaagcccc atatatgaaa aacgcaacgt tctaccttct ggacaatgac accaccgtcg
    45301 atggcttaag cgccgttgag caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg
    45361 gtaagcgcgt actcatcgcc tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc
    45421 tgtgggcgcg tccggcagaa agctttgttc cgcataattt agcgggagaa ggaccgcgcg
    45481 gcggtgcgcc ggtggagatc gcctggccgc aaaagcgtag cagcagcccg cgcgatatat
    45541 tgattagtct gcgaacaagc tttgcagatt ttgccaccgc tttcacagaa gtggtagact
    45601 tcgttcctta tgaagattct ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg
    45661 tggctggttt caacctgaat acggcaacct ggaaataatg gaaaagacat ataacccaca
    45721 agatatcgaa cagccgcttt acgagcactg ggaaaagcag ggctacttta agcctaatgg
    45781 cgatgaaagc caggaaagtt tctgcatcat gatcccgccg ccgaacgtca ccggcagttt
    45841 gcatatgggt cacgccttcc agcaaaccat catggatacc atgatccgct atcagcgcat
    45901 gcagggcaaa aacaccctgt ggcaggtcgg tactgaccac gccgggatcg ctacccagat
    45961 ggtcgttgag cgcaagattg ccgcagaaga aggtaaaacc cgtcacgact acggccgcga
    46021 agctttcatc gacaaaatct gggaatggaa agcggaatct ggcggcacca ttacccgtca
    46081 gatgcgccgt ctcggcaact ccgtagactg ggagcgtgaa cgcttcacta tggacgaagg
    46141 cctgtccaat gcggtgaaag aagttttcgt tcgtctgtat aaagaagacc tgatttaccg
    46201 tggcaaacgc ctggtaaact gggacccgaa actgcgtacc gctatctctg acctggaagt
    46261 ggaaaaccgc gaatcgaaag gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc
    46321 gaaaaccgca gatggtaaag attatctggt ggttgcgact acccgtccgg aaaccctgct
    46381 gggcgatacc ggcgtggccg ttaacccgga agatccgcgt tacaaagatc tgattggcaa
    46441 atatgtcatt ctgccgctgg ttaaccgtcg tattccgatc gttggcgacg aacacgccga
    46501 catggaaaaa ggcaccggct gcgtgaagat caccccggcg cacgacttta acgactatga
    46561 agtgggtaaa cgtcacgccc tgccgatgat caacatcctg acctttgacg gtgatatccg
    46621 tgaaagcgcc caggtgttcg ataccaaagg taacgaatct gacgtttatt ccagcgaaat
    46681 cccggcagag ttccagaaac tcgagcgttt tgccgcacgt aaagcggtcg ttgctgcggt
    46741 tgacgcactc ggcctgctgg aagaaattaa accgcacgac ctgaccgttc cttacggcga
    46801 ccgtggcggc gtagttatcg aaccgatgct gaccgaccag tggtacgtgc gtgccgatgt
    46861 cctggcgaaa ccggcggttg aagcggttga gaacggcgac attcagttcg taccgaagca
    46921 gtacgaaaac atgtacttct cctggatgcg cgatattcag gactggtgta tctctcgtca
    46981 gctgtggtgg ggtcaccgta tcccggcatg gtacgacgaa gcgggtaacg tttatgttgg
    47041 ccgcaacgaa gacgaagtgc gtaaagaaaa taacctgagc gcggacgtcg cgctgcgtca
    47101 ggacgaagac gttctcgaca cctggttctc ctccgcgctg tggaccttct ccacgctcgg
    47161 ctggccggaa aacaccgacg cactgcgcca gttccaccca accagcgtga tggtatccgg
    47221 cttcgacatc atcttcttct ggattgcccg catgatcatg atgaccatgc acttcatcaa
    47281 agatgaaaat ggcaaaccgc aggtgccgtt ccacaccgtt tacatgaccg gcctgattcg
    47341 tgatgacgaa ggccagaaga tgtccaaatc caagggtaac gtgattgacc cgctggatat
    47401 ggttgacggt atttcgctac cggaactgct ggaaaaacgt accggcaata tgatgcagcc
    47461 gcagctggcg gacaaaatcc gtaagcgcac cgagaagcag ttcccgaacg gcattgagcc
    47521 gcacggtact gacgcgctgc gcttcaccct ggcggcgctg gcttctaccg gtcgtgacat
    47581 caactgggat atgaagcgtc tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc
    47641 cagccgcttt gtgctgatga acacagaagg tcaggattgc ggcttcaacg gcggcgaaat
    47701 gacgctatcg ctggcggacc gctggattct ggcggagttc aaccagacca tcaaagcgta
    47761 ccgcgaagcg ctggacagct tccgcttcga tatcgccgca ggcattttgt atgagttcac
    47821 ctggaaccag ttctgtgact ggtatctgga gctgaccaag ccggtaatga acggtggaac
    47881 agaagccgaa ctgcgcggta ctcgccatac gctggtgact gtactggaag gtctgctgcg
    47941 cctcgcgcat ccgatcattc cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact
    48001 ttgcggtatc actgccgaca ccatcatgct gcagccattc ccgcagtatg atgcatctca
    48061 ggttgatgaa gccgcactgg ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg
    48121 taacatccgt gcagaaatga acatcgcgcc gggcaaaccg ctggagctgt tgcttcgtgg
    48181 ttgcagcgcg gatgcagagc gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc
    48241 gcgtctggaa agcatcaccg tgctgcctgc cgatgacaaa ggtccggttt ccgttaccaa
    48301 gatcatcgac ggcgcagagc tgctgatccc gatggctggc ctcatcaaca aagaagatga
    48361 gctggcgcgc ctggcgaaag aagtggcgaa gatcgaaggt gaaatcagcc gcatcgagaa
    48421 caagctggca aatgaaggct ttgtcgcccg cgcaccggaa gcggttatcg cgaaagagcg
    48481 tgagaagctg gaaggttatg cagaagcgaa agccaagctg attgaacagc aggctgttat
    48541 cgccgcactg taatcaacac agtgaagaaa acgaaggccg gagcatgctc cggccttttt
    48601 tatctcttac agaaatggta aagaaggcat aactccccga gaaatactgg ctaagaagcc
    48661 tttatctggt tgttcttctt tattgattaa aggaaggtca tccaaatcgc ccagcaaata
    48721 cgtatttttt gcctgccagt caatcatcca tattttcagc agtggataag ccagaccgcc
    48781 cgtaatcccg gatatcacca ccaacgcaca catgcgataa agcataccgt ggtacgttaa
    48841 agttgagcga aaacgaatac gcccatcatt cagtgacagg ttgctcataa aatgatttcg
    48901 caacgacacc gttaaataac tggtgctaac agcaatccca aaataataga ttaactgcgc
    48961 gattatcatt ttacgttgca tttccataaa ttgctgtaaa ttctcaatgt catcatttgc
    49021 atatacagaa ctatcatacg catttaatat ttgatcgaag ataatataac cagcaacagc
    49081 gagaaaaggc aataaagcta aaaatgcgag aatggcatat tttatacaat acgtagtatc
    49141 taatttcacc ttgaaacgat gtataccgaa actggtattg ctccagagaa agctcattac
    49201 caggctatat aaagtaccat taaaaatacc aatggaaaca ataccaacta ttgccatcag
    49261 aaccacagat ataataacac tacttgaact attggcaggt agcatctttg tcgagataaa
    49321 gaaaacagtc cccatcccaa tggccattaa aatcggcaag aaaaaggtta cccaccagaa
    49381 ccctttcaaa gagcaattaa aactaaatct tacgccgttg agactggtca ttaaggcctg
    49441 ataacgcagt ccttttgctg ccataaatat aagaaaaacc aacagtaaca aagtcaaaac
    49501 acagccaaca agcggcatat ctgctgacac tgtcattaag attgcgaaat agaaaaaaac
    49561 aaaaacaaga caactaacaa aaacattccc accggtaatt ccataagaaa atcgttgtcc
    49621 gttaacttcc atattagcat aaagataacg cttacatttc attaatgccc atggtaaata
    49681 gatacctagc gttataatcg ttaacaacac attcaccaca caaataagaa aatatctctc
    49741 acctgtacca tgaaaaacaa aagaatggga cggaacatcc atttcattaa taacttgagc
    49801 cataaaaatt ccctgaatta aaaaatccat tgatataatt tatagataca gacagtaaaa
    49861 tgttctgcca cgttacaata ctcttatcag taataggctg gcaaaaactt tattgcttta
    49921 taagacgctt gcgcacgtaa gaagagagtg gtattaaaca aatgtataat tactttattt
    49981 ttattgggtt aaactatgaa taacattgcg ccgcaatcac ctgtaatacg tcgcctgacg
    50041 ctgcaggata atcctgctat cgcccgcgtc attcgtcagg tatccgccga atacggtctt
    50101 accgctgata aaggctacac cgtcgccgat ccgaatcttg acgagctgta tcaagtgtat
    50161 agtcagcctg gccatgcata ttgggtggtt gagtacgagg gtgaagtggt cggcggcggt
    50221 gggatagcgc cattaacagg tagtgaagcg gacatttgcg aactgcagaa aatgtacttt
    50281 cttcccgcca ttcgcggtaa agggctggca aaaaaactgg ccttaatggc gatggagcag
    50341 gcgcgagaaa tgggtttcaa acgctgctat ctggaaacga ccgctttttt gaaagaagcg
    50401 attggtttat atgaacattt aggttttcaa catatcgact atgcacttgg ctgcacgggc
    50461 catgttgatt gtgaagtgcg gatgttgcgc gaactctgaa aaaatgcccg ctagtatgtg
    50521 ctatcgggca ttttaatcaa ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa
    50581 atcttcatcg tcgccatctt cgccgttagg atcttcaaag taagtgcccc aaccgtcgta
    50641 ctcaacgtca aatttctccg ccagcgtcat cagttgttca acctgggcat cgatcagatc
    50701 ggcattcaac gcgcactcgc tgaggatgtc gcagcaaatc acgatatcac catcttcaac
    50761 ttccagctct tctggatcgg tcacttcgta accgagttta aacgcttcaa ctgccgcttt
    50821 ttccagagtt tccagatcgt ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg
    50881 atcgctgcca tcttccagta attcttcaat aatcaaacgt gtttcttcac gctgttcttc
    50941 cagttgttcc gggtttgcca tggctcattc ctcttaatgt cctgccgata ctcttattgt
    51001 cacacaccac tgacattgcc tccacctttg tgataaagat ttatgcttta gacttgcaaa
    51061 tgaataatca tccatataaa ttgaatttta attcattgag gcgttagcca caggagggat
    51121 ctatgtccgg gttttatcat aagcatttcc tgaaattact cgatttcacg ccagctgaac
    51181 ttaacagcct gctgcagtta gccgcgaagc tgaaagctga taagaaaagc ggtaaagaag
    51241 aagccaaact cactggtaaa aacatcgcgc tcatcttcga aaaagactcg acccgtaccc
    51301 gatgctcttt cgaagttgcc gcatatgacc agggtgctcg cgttacttat ctcggcccaa
    51361 gcggtagtca gattggtcat aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca
    51421 tgtatgacgg tattcagtat cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat
    51481 acgctggcgt gccggtatgg aacggcctga ccaatgagtt tcaccccacg cagttgctgg
    51541 cggatctcct caccatgcag gagcatttac ccggtaaagc attcaacgaa atgacgctgg
    51601 tctatgcagg tgacgcacgt aacaacatgg gcaattcgat gctcgaagct gcggcgctta
    51661 ccggtctgga tttgcgtctg gtcgcgccac aggcatgctg gccagaagct gcgctggtta
    51721 cggaatgccg cgccctggca caacaaaatg gcgggaatat tacgctgacg gaagatgtag
    51781 ctaaaggagt tgaaggtgct gactttatct ataccgatgt atgggtgtcg atgggtgaag
    51841 caaaagaaaa atgggctgaa cgcattgcat tactgcgtga ttatcaggtg aacagcaaga
    51901 tgatgcagtt gaccggtaac ccggaggtca aattcctcca ctgcctgccc gcgtttcatg
    51961 acgaccagac gacgcttggt aagaaaatgg cggaagagtt tggcctacat ggcggaatgg
    52021 aagtgactga tgaggtcttc gaatctgccg ccagcattgt ttttgatcag gcggaaaacc
    52081 gcatgcatac catcaaagcg gtgatggt
//
LOCUS       DAZGHF010000017        49354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGHF010000017 DAZGHF010000000
VERSION     DAZGHF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.017"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            80..646
                     /locus_tag="ACHK3A_001127"
     CDS             80..646
                     /locus_tag="ACHK3A_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO8739241.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            795..1916
                     /locus_tag="ACHK3A_001128"
     CDS             795..1916
                     /locus_tag="ACHK3A_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO8739242.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            1916..5023
                     /locus_tag="ACHK3A_001129"
     CDS             1916..5023
                     /locus_tag="ACHK3A_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO8739243.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVENVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            5027..6400
                     /locus_tag="ACHK3A_001130"
     CDS             5027..6400
                     /locus_tag="ACHK3A_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO8739244.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAAQETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQIGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            6409..7572
                     /locus_tag="ACHK3A_001131"
     CDS             6409..7572
                     /locus_tag="ACHK3A_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO8739245.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFIRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(7621..8427)
                     /gene="sapF"
                     /locus_tag="ACHK3A_001132"
     CDS             complement(7621..8427)
                     /gene="sapF"
                     /locus_tag="ACHK3A_001132"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO8739246.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(8429..9421)
                     /gene="sapD"
                     /locus_tag="ACHK3A_001133"
     CDS             complement(8429..9421)
                     /gene="sapD"
                     /locus_tag="ACHK3A_001133"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO8739247.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(9421..10311)
                     /gene="sapC"
                     /locus_tag="ACHK3A_001134"
     CDS             complement(9421..10311)
                     /gene="sapC"
                     /locus_tag="ACHK3A_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO8739248.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(10298..11263)
                     /gene="sapB"
                     /locus_tag="ACHK3A_001135"
     CDS             complement(10298..11263)
                     /gene="sapB"
                     /locus_tag="ACHK3A_001135"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO8739249.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(11260..12903)
                     /gene="sapA"
                     /locus_tag="ACHK3A_001136"
     CDS             complement(11260..12903)
                     /gene="sapA"
                     /locus_tag="ACHK3A_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO8739250.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(13216..13461)
                     /gene="ymjA"
                     /locus_tag="ACHK3A_001137"
     CDS             complement(13216..13461)
                     /gene="ymjA"
                     /locus_tag="ACHK3A_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO8739251.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(13595..14980)
                     /gene="puuP"
                     /locus_tag="ACHK3A_001138"
     CDS             complement(13595..14980)
                     /gene="puuP"
                     /locus_tag="ACHK3A_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO8739252.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            complement(14970..15134)
                     /locus_tag="ACHK3A_001139"
     CDS             complement(14970..15134)
                     /locus_tag="ACHK3A_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739253.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(15283..16701)
                     /gene="puuA"
                     /locus_tag="ACHK3A_001140"
     CDS             complement(15283..16701)
                     /gene="puuA"
                     /locus_tag="ACHK3A_001140"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO8739254.1"
                     /translation="METNIVEVENFVQQSEERRGSSFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            16913..17677
                     /gene="puuD"
                     /locus_tag="ACHK3A_001141"
     CDS             16913..17677
                     /gene="puuD"
                     /locus_tag="ACHK3A_001141"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO8739255.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            17704..18261
                     /gene="puuR"
                     /locus_tag="ACHK3A_001142"
     CDS             17704..18261
                     /gene="puuR"
                     /locus_tag="ACHK3A_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO8739256.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            18411..19898
                     /gene="puuC"
                     /locus_tag="ACHK3A_001143"
     CDS             18411..19898
                     /gene="puuC"
                     /locus_tag="ACHK3A_001143"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO8739257.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVEVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            19900..21180
                     /gene="puuB"
                     /locus_tag="ACHK3A_001144"
     CDS             19900..21180
                     /gene="puuB"
                     /locus_tag="ACHK3A_001144"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO8739258.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVHELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            21218..22483
                     /gene="puuE"
                     /locus_tag="ACHK3A_001145"
     CDS             21218..22483
                     /gene="puuE"
                     /locus_tag="ACHK3A_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO8739259.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKMFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDEAMKILQDALSD"
     gene            complement(22603..23580)
                     /gene="pspF"
                     /locus_tag="ACHK3A_001146"
     CDS             complement(22603..23580)
                     /gene="pspF"
                     /locus_tag="ACHK3A_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO8739260.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            23747..24415
                     /gene="pspA"
                     /locus_tag="ACHK3A_001147"
     CDS             23747..24415
                     /gene="pspA"
                     /locus_tag="ACHK3A_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO8739261.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            24469..24693
                     /gene="pspB"
                     /locus_tag="ACHK3A_001148"
     CDS             24469..24693
                     /gene="pspB"
                     /locus_tag="ACHK3A_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO8739262.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            24693..25052
                     /gene="pspC"
                     /locus_tag="ACHK3A_001149"
     CDS             24693..25052
                     /gene="pspC"
                     /locus_tag="ACHK3A_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO8739263.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            25061..25282
                     /gene="pspD"
                     /locus_tag="ACHK3A_001150"
     CDS             25061..25282
                     /gene="pspD"
                     /locus_tag="ACHK3A_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO8739264.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            25357..25671
                     /gene="pspE"
                     /locus_tag="ACHK3A_001151"
     CDS             25357..25671
                     /gene="pspE"
                     /locus_tag="ACHK3A_001151"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO8739265.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            25885..27564
                     /gene="ycjM"
                     /locus_tag="ACHK3A_001152"
     CDS             25885..27564
                     /gene="ycjM"
                     /locus_tag="ACHK3A_001152"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO8739266.1"
                     /translation="MEQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSTFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYIRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     IDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNNKATLRYLIYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            27578..28870
                     /gene="ycjN"
                     /locus_tag="ACHK3A_001153"
     CDS             27578..28870
                     /gene="ycjN"
                     /locus_tag="ACHK3A_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8739267.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITVGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQTSQKKLDELVEQR"
     gene            28891..29772
                     /gene="ycjO"
                     /locus_tag="ACHK3A_001154"
     CDS             28891..29772
                     /gene="ycjO"
                     /locus_tag="ACHK3A_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO8739268.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            29759..30601
                     /gene="ycjP"
                     /locus_tag="ACHK3A_001155"
     CDS             29759..30601
                     /gene="ycjP"
                     /locus_tag="ACHK3A_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO8739269.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            30632..31684
                     /gene="ycjQ"
                     /locus_tag="ACHK3A_001156"
     CDS             30632..31684
                     /gene="ycjQ"
                     /locus_tag="ACHK3A_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO8739270.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCEIARRHGADFCLNPIGSDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            31703..32491
                     /gene="ycjR"
                     /locus_tag="ACHK3A_001157"
     CDS             31703..32491
                     /gene="ycjR"
                     /locus_tag="ACHK3A_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO8739271.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVITACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEKVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALLEQLRADNFQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            32501..33556
                     /gene="ycjS"
                     /locus_tag="ACHK3A_001158"
     CDS             32501..33556
                     /gene="ycjS"
                     /locus_tag="ACHK3A_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO8739272.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            33553..35820
                     /gene="ycjT"
                     /locus_tag="ACHK3A_001159"
     CDS             33553..35820
                     /gene="ycjT"
                     /locus_tag="ACHK3A_001159"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO8739273.1"
                     /translation="MIRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWCTSNGAGYTITSRRFVSADQLPLIALEITITPLDADALVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKRVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQATGDESFIAHEGMALLLGTAK
                     FWISRAVQVNDRLEIHNVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARKFGCSDDA
                     FIHRAEIFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRNGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            35817..36476
                     /gene="pgmB"
                     /locus_tag="ACHK3A_001160"
     CDS             35817..36476
                     /gene="pgmB"
                     /locus_tag="ACHK3A_001160"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO8739274.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     ADLRAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            36490..37572
                     /locus_tag="ACHK3A_001161"
     CDS             36490..37572
                     /locus_tag="ACHK3A_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8739275.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKERNIAMVFLNYALYPHMTVYDNMAFGLK
                     MQKIANEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTISEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            37617..38522
                     /gene="ompG"
                     /locus_tag="ACHK3A_001162"
     CDS             37617..38522
                     /gene="ompG"
                     /locus_tag="ACHK3A_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO8739276.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(38573..39571)
                     /gene="ycjW"
                     /locus_tag="ACHK3A_001163"
     CDS             complement(38573..39571)
                     /gene="ycjW"
                     /locus_tag="ACHK3A_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8739277.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGNQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLIAGKPSPQNITVATHMSLK"
     gene            39726..41123
                     /gene="ycjX"
                     /locus_tag="ACHK3A_001164"
     CDS             39726..41123
                     /gene="ycjX"
                     /locus_tag="ACHK3A_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO8739278.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            41120..42181
                     /gene="ycjF"
                     /locus_tag="ACHK3A_001165"
     CDS             41120..42181
                     /gene="ycjF"
                     /locus_tag="ACHK3A_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO8739279.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIEDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            42329..43870
                     /gene="tyrR"
                     /locus_tag="ACHK3A_001166"
     CDS             42329..43870
                     /gene="tyrR"
                     /locus_tag="ACHK3A_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO8739280.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(43914..44420)
                     /gene="tpx"
                     /locus_tag="ACHK3A_001167"
     CDS             complement(43914..44420)
                     /gene="tpx"
                     /locus_tag="ACHK3A_001167"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO8739281.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            44539..45504
                     /gene="ycjG"
                     /locus_tag="ACHK3A_001168"
     CDS             44539..45504
                     /gene="ycjG"
                     /locus_tag="ACHK3A_001168"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO8739282.1"
                     /translation="MRTVKVFEEGWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQESLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(45479..46207)
                     /gene="mpaA"
                     /locus_tag="ACHK3A_001169"
     CDS             complement(45479..46207)
                     /gene="mpaA"
                     /locus_tag="ACHK3A_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO8739283.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPL"
     gene            complement(46498..47136)
                     /locus_tag="ACHK3A_001170"
     CDS             complement(46498..47136)
                     /locus_tag="ACHK3A_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8739284.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKAGDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(47207..48127)
                     /gene="ycjY"
                     /locus_tag="ACHK3A_001171"
     CDS             complement(47207..48127)
                     /gene="ycjY"
                     /locus_tag="ACHK3A_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8739285.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNSRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSREYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            48265..49164
                     /gene="pgrR"
                     /locus_tag="ACHK3A_001172"
     CDS             48265..49164
                     /gene="pgrR"
                     /locus_tag="ACHK3A_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO8739286.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT        12051 a        12245 c        13241 g        11817 t
ORIGIN      
        1 ttccattact ttggagaaac gtataattag caaaaatgag aacgatcatt ctcatcaagt
       61 gataggccag gagacagaaa tgaccagcaa gctggagata cgccacaaac agcgtcagga
      121 tgaaatcatt aacgccgccc gtcggtgttt tcgtcgctgc ggatttcatg ccgccagtat
      181 gtcgcaaatt gccagcgaag ctcagcttag cgtgggtcag atatatcgtt acttcgccaa
      241 caaagacgcc attattgaag agatggttcg ccgtatcatc gactttcgta ttgcccaaat
      301 ggacattgac gcgcgtaccg atcacttccc ggaagtcctt gccttgcgta aatccttaaa
      361 tgaggacgac gacgcgctga tgctggaagt ggctgcagaa gccacacgca acccgcgggt
      421 tatggcaatg cttgaagaag ctgacgccag aatgttcgct aacggttgtg cacatatgaa
      481 aagaatgcat ccccatctta gcgacgagca cattcgttgc tgcgttgaag tgtttgctac
      541 catgatggaa ggtacggttt atcgtcgttt aacaccgcaa aaaagcgatc cacaacatct
      601 gcaggaaata tatcaggata ttgttagtat gttgataaat aaataagaac agaattaacc
      661 aatagcgggg gatattaatc ccccttattt cattgcgaaa ttcatttcgc gatgcgggtt
      721 cgttataact gaatatccct gacatatcct cattaatttc aatgaggagg gtatttctca
      781 ccctggagaa aataatgaag tatatagcaa catctgtagt ggcaatgctg ctcttatcgg
      841 gttgtgataa tacgcaaagt aacaattcat ccccgtcaga aacagaagtc ggcgttgtta
      901 cgctcaaatc tcaaccggtt tcggtagtca gtgaattaac cggacgcacc agtgctgcgc
      961 tcagtgctga agtacgtccg caggttgggg gaattatcca gaaacgctta tttaaggaag
     1021 gtgatctggt caaggctgga cagccgctct accagattga tgcggccagt tatcaggctg
     1081 catggaatga agctcgggca gcattacaac aagcacaggc actggtaaaa gccgattgcc
     1141 agaaagcgca gcgttatgcc cgactggtga aagagaacgg tgtttcacaa caggatgctg
     1201 atgatgctca gtctacctgt gcacaagata aagccagtgt agaggcgaaa aaagccgcac
     1261 tggaaactgc gcgcattaat cttgactgga ccacggtaac cgcaccgatt tcggggcgta
     1321 ttggtatttc gtcggtaacc cctggcgcac tggtgaccgc gtcgcaggat acagcgttaa
     1381 cgactattcg tggtctggat acaatgtatg tcgacctcac tcgctctagt gtcgatttat
     1441 tacgtctgcg taaacagtca ctggcgacca acagtgacac catgagcgtc tcacttattc
     1501 tggaagatgg cacaacttac agcgaaaaag ggcgtctgga actcaccgaa gtcgctgtag
     1561 atgagtctac cggttcggtg acattacggg cgatttttcc caatccacaa cagcagttat
     1621 taccgggaat gtttgttcgc gctcgtgtcg atgaaggcgt gatggaagac gctattctcg
     1681 cgccgcaaca gggcgtcacg cgcgatgcta aaggcaatgc aactgcgctg gtggtgaata
     1741 aagacaataa agtagagcag cgaacgctcg aaacgggaga aacgtatggt gataaatggc
     1801 tggtgctgaa cggcctgcac aacggcgacc gactgattgt tgaaggttct gccaaagtca
     1861 cttcagggca gaccgtcaag gctgttgaag ttcaggctaa tggaggcaac gcctgatgtt
     1921 ttcgcgcttt tttgttcgcc gcccggtctt tgcctgggtt atcgccattt tgattatgtt
     1981 ggcggggatt ctggctattc gcacattgcc cgtcgcgcaa tatcctgacg ttgcgccacc
     2041 gaccattaaa atttcagcca cttatactgg tgcttctgcc gaaacgctgg agaacagcgt
     2101 gactcaggtt atcgagcaac aactcactgg gcttgataat ttactctatt tcagctcaac
     2161 cagcagctct gatggttcgg tcagtattaa tgtgaccttt gaacaaggta ccgatccaga
     2221 tactgcacag gttcaggtgc agaataaaat tcagcaggcg gagtcgcgcc tacccagcga
     2281 agtgcagcaa acgggtgtta cggtggagaa atcacaaagc aactttttgc tgattgctgc
     2341 cgtgtatgac accactgaca aagcctccag ttcggatatc gccgactggt tggttagtaa
     2401 cgttcaggac ccgctggcgc gtgttgaagg tgtggggagt ctgcaagtct ttggcgcgga
     2461 atacgctatg cgcatctggc ttgacccggc caaactggcg tcttactcgc taatgccttc
     2521 agacgtgcaa agtgctattg aagcgcaaaa cgtgcaggtt actgccggga aaataggggc
     2581 attgccttca ccgaatactc agcaactgac cgcaacggta cgtgcgcagt ctcgtttgca
     2641 gacggtggat cagttcaaaa atattatcgt gaaaagccag tcagacggcg cagttgttcg
     2701 tataaaagat gtggctcgcg ttgagatggg cagtgaagat tataccgcta tcggcaaact
     2761 taacggtcac ccgtctgccg gggttgctgt aatgctttcg cccggtgcga atgcgctgaa
     2821 tacggcgacg ctggtcaagg ataagattgc cgaattccag cggaacatgc cgcagggata
     2881 cgacatcgca tacccgaaag acagcactga atttattaaa atctccgtag agaacgtaat
     2941 tcagacgctg tttgaagcca tcgtattggt ggtttgcgtg atgtatttat tcctgcaaaa
     3001 cctgcgtgcc acactgattc cggcgttagc tgtccccgtc gttttgctgg gcacattcgg
     3061 cgttcttgcg ttgtttggct attcgattaa taccctgacg ttgtttgcaa tggtgctggc
     3121 gatcggcttg ctggtggacg atgccattgt ggtggtagag aacgtcgaac gcattatgcg
     3181 tgacgaaggg ttgcccgcgc gtgaagccac ggaaaaatca atgggcgaga tttctggcgc
     3241 actggttgcc attgcgctgg tgttgtcagc cgtattcctg ccgatggcct tctttggggg
     3301 atctacgggc gtaatttatc gtcagttctc catcaccatc atctccgcaa tgctgctttc
     3361 cgtggtggtg gcattgacct tgactcccgc cctgtgcggt tccgtcctcc agcatgttcc
     3421 gccacataaa aaaggctttt tcggcgcatt taaccgcttt taccgccgta ctgaagataa
     3481 atatcagcga ggcgtaattt atgtcctgcg ccgtgctgct cgaacgatgg ggctttatgt
     3541 cgtactgggt ggtggaatgg ccctgatgat gtggaaactg ccgggcagtt tcttacccac
     3601 tgaagaccag ggcgaaatca tggtgcagta cacgctgccg gccggggcga ccgctgcccg
     3661 tacagcagaa gtgaatcgcc agattgttga ctggttcctg attaacgaga aagcaaatac
     3721 cgatgtcatt tttaccgttg atggtttcag ttttagcggc agcggacaga acaccgggat
     3781 ggcgtttgtt tcgttgaaaa actggtctca acgtaaaggg gcagaaaaca ccgcccaggc
     3841 tatcgcccta cgggctacca aagagctggg cacaattcgt gatgccacgg tatttgcgat
     3901 gacgccgcca gccgttgatg ggctgggcca aagcaatggt tttacgtttg aattgttagc
     3961 taacggtgga accgatcgcg aaacactact gcaaatgcgt aatcagttga tagaaaaagc
     4021 gaatcaaagt ccggagttac attctgtgcg tgccaatgat ttgccacaaa tgccgcaatt
     4081 gcaggtagat attgatagta ataaagcggt gtcattaggg ttgagtttga atgatgtcac
     4141 cgacaccctg tccagtgcgt ggggcggtac ttatgtgaat gactttattg accgtggtcg
     4201 tgtgaaaaaa gtctacattc agggcgacag tgaatttcgt tccgcgccgt cagacttagg
     4261 taaatggttt gtgcgcggta gcgataacgc catgacacca ttctctgctt ttgcgaccac
     4321 ccgctggctg tatggaccgg aaagactggt gcgctacaac ggttctgcag cctatgaaat
     4381 tcagggggaa aatgcgactg gctttagttc cggcgatgca atgacaaaaa tggaggaact
     4441 ggcaaacagt cttcctgcgg gaacaacctg ggcctggagt ggtttgtcat tgcaggagaa
     4501 actggccagc ggtcaggcat tgagtctgta tgcagtttct attctggtgg ttttcctctg
     4561 ccttgcagca ctgtacgaaa gctggtcagt cccgttctcg gtcattctgg tgatccctct
     4621 ggggcttctt ggcgcggcgc tggcggcctg gatgcgtgat ttaaacaacg atgtttactt
     4681 ccaggtggcg ctattaacca ctatcggctt gtcgtcgaaa aacgccattc tgattgtgga
     4741 atttgctgaa gcggcggttg cagagggcta ttccttgagt cgtgcggcat tacgcgcggc
     4801 gcagactcgt ttacgcccaa tcatcatgac ctcgctggcg tttattgcgg gggtaatgcc
     4861 gctggcgata gcaaccggtg caggggcgaa cagccgcatc gccattggta cgggcattat
     4921 tggcggcacg ctgaccgcta cattactggc tatttttttt gttcctctgt tttttgtact
     4981 ggtgaaacgt ttgtttgccg gtaaaccgcg ccgtcaggag taagttatgt tgcgtcgttc
     5041 tcttattttt ctggtgttgt tgagtgcggg atgtgtctcg ctcgatcccc actacagtac
     5101 gcctgaatcc ccaatcccgg cgaccttgcc gggagcacag ggccagggga aggcaatcag
     5161 ccatgactgg cagcaggtga tccacgatcc ccgattgcag caggtagtaa ccattgcact
     5221 gaacagtaac cgcgatgtgc aaaaagcgat tgctgatatt gactcagcac gggcgctcta
     5281 tgggcaaact aatgcgtcgt tatttccgac ggtgaatgcg gccttaagtt cgactcgcag
     5341 tcgttcgctg gcgaatggta cgggaacaac tgccgaggct gacggcacgg tgtccagtta
     5401 tacgttagat ctgtttggtc gtaatcagag tttatctcgg gcggcgcagg aaacctggct
     5461 tgccagtgaa tttacagccc agaacactcg tttaacgctg attgctgaaa tcagcaccgc
     5521 ctggctgaca ctggcggctg ataacagcaa cctggccctg gcaaaagaaa cgatgaccag
     5581 tgcagaaaac tcattgaaaa ttatccagcg ccagcaacag attggcacgg cggcagcgac
     5641 agatgtcagt gaggcgatga gtgtttatca gcaagcgcgc gccagcgttg ccagctatca
     5701 gacacaagtg atgcaggata aaaatgcgct gaacttactg gcaggtacaa cgcttgcgga
     5761 aaatctgcta ccgggaacac tggaaagcct gccggaacaa atgataagtc tggttcctgc
     5821 gggagtatca tctgatgttc tgctgcgtcg cccggatatc caggaagctg aacataattt
     5881 gaagagcgcc aatgcagata ttggcgcggc gcgggctaac tttttcccga cgatttcgct
     5941 gactgccagt gccggtgttg gcagtgacgc attgtcgtcg ttgttcagcc acggaatgca
     6001 gatctggtca tttgcgccat cggtcacatt accgctgttc actggcggaa gtaatctggc
     6061 gcagcttcgt tacgcagaag cacaaaagcg tgggctcatt gccacctatg aaaaaaccgt
     6121 tcagagcgca tttaaagatg tcgctaacgc actggcgcgg cggaccacgc ttgaagagca
     6181 actggatgca cagcgccagt atgttaaagc tgagcaacaa acggtcgatg ttggcttgcg
     6241 tcgctatcag gcgggtgttg gtgattacct gaccgtgttg acggcgcagc gcagcttgtg
     6301 gagcgcacag caggagctgc tggcactgca actgactgat tttacaaacc gaatcacgct
     6361 ctggcagtcg ctgggcggcg gtatgtcatc actcaagtaa ggaataatat ggctagagtc
     6421 tctctttcat gggcattgat tcttggtctt ttagccggta tcggcccgat gtgtaccgat
     6481 ctttatttgc cggctttgcc ggagatgtct gagcaactgg cggcaaccac gaccataacg
     6541 caattaaccc tgaccgcatc actgattggt cttggcgtcg gacaactgtt atttggccct
     6601 ctgagcgaca aaatagggcg taaacgtccg ttaatcttgt cgttgctatt gtttattgtt
     6661 tcttccattt tatgcgcgac aacgaacaat atttactggc tggtggtctg gcgttttatt
     6721 caagggatcg cgggggcggg tggttcggtg ctctctcgtt ctattgctcg tgacaaatat
     6781 cagggagtaa cgttgaccca gttttttgcg ctgttaatga cggtgaatgg cctggcaccg
     6841 gtgttgtcgc cagtgctggg cgggtacatt gttagcactt ttgactggcg cactttattc
     6901 tgggtaatgg ctgaaattag caccgtactg ttgctgggct gcctgttatt tattaatgag
     6961 accttgccag aaaataaaag gggctcatca ttgctattaa ccggacgaag cgtggtgcag
     7021 aaccgccgtt ttatacgctt ttgcctgatt caaagtttta tgctggctgg tttgtttgca
     7081 tatatcggct cttcgtcgtt cgtgttgcag aaggaatttg gctttagtcc aatgcaattt
     7141 agcctggtgt ttggccttaa cggcatcgga cttatcattg cttcatggat cttctcgcgc
     7201 ctggcgcgac ggattaacgc gatgacattg ttgcgaggtg gcctgatagc ggcaattttg
     7261 tgtgcattgc tcacggtctt atgcgcatgg atacaattgc ccattccggc actggtggca
     7321 ttatttttca ccatcgcatt ttgtagcggc atcggcactg ttggcggggc agaggctatg
     7381 agtgcagtag ggacgcagga atctggaacg gcgtctgcgt tgatggggat gagcatgttt
     7441 gtcttcggcg gtatagccgc gccattgtcg ggaattggcg gagaaacact gttaaaaatg
     7501 agtctggcaa ttacggtgtg ttatacgctg gcattgctgg ttgctctcac cagaatcgac
     7561 aatcaaaagt aatgaacacc tcatcctgag gggggccctg tcaggatgag gtgcaaacca
     7621 ctaacggtcc ttacgccacg catccgccgt caatgcctcg ccaaaatgac cggcaataag
     7681 ccgtttggtg agttcatgca gcggcgatgc cagcacatcg gcggtgctgc ctcgctcgac
     7741 aacctcgccc tgatgcatca ccagtacctg gtcgctaatg tgcttcatca ttccgatatg
     7801 ctgggtaaca taaatatacg aaataccctg tttttcctgt aattccagca tcagattaat
     7861 caactgcgaa cgcatcgaca tatccagtga ggcgagggct tcatcggcaa taatcacttt
     7921 tggacgcaat atcagcgcgc gtgccagacc cagacgttgt ttttgtccgg gcgccaacat
     7981 atgcggatag taactgacgt gatccggtag cagcccaacc atccgcatcg tttcaataat
     8041 ctgtttgcga cgctgttcag gttccagatc ggtgttcaag cgcagtggaa aatcaagaat
     8101 ctgcgagata cgttgacggg gattcaacga ggtcgaagga tcctgaaaaa tcatgcgaat
     8161 acgctgacta cggaaggaat aatcgccaaa atgcagcgga tgatcgtcaa tcaataactc
     8221 gccgctggtc ggttctatca ttcccgccag catttttgcc agtgtggatt tacccgaccc
     8281 attctcacca ataatcgcca gtgtctggcc ttcacgtagc gtaaagctca aaggttttac
     8341 cgcttctacg gtctgacgac gaaaccagcc ggtccggtaa cggaacgttt tacttaaatt
     8401 acgcacttca agcagcgttt cgatcatctc actctttctc catgttcagc gggaagtgac
     8461 aggcatagag atgatttttc gcccccgtca aacgtggcgt cacaatgcat tctcgttgtg
     8521 catacgggca acgcggcccc agacgacacc caatcggtaa ctgttccagc agtgggatag
     8581 cgccgggcag cgtattgagg cgacttttat gcggcatcgc gctgccgaag tctggtatcg
     8641 cgcggatcag cgcctgggta taaggatgat gtggcattgt caccaactcc ttacttggcg
     8701 cggtttccac cgtttgaccg cagtaaagca cgttaatttt atccgcccat tggctaagca
     8761 tttgtaagtc atggctgata agcaaaatag tggtattgct gttctggttg agacgcgtca
     8821 gcaggcgaaa gatttgcgcc tgggttgttg gctccattga gtttgtcggt tcgtcagcaa
     8881 tcagcagacg cggttgattc gccagtgcaa tggctatcat cactttctga cattcacctt
     8941 cggtcaactc atagggaaaa ctgcgcatcg catctttgtg atctttaatc cccacacggt
     9001 gcagtagttc aatggcacgg cgtttgcgcc agccaaaacg ttgccaccaa cggcctttat
     9061 acgtccaggc tgggatgttt tgcatcaact ggcgacccac acgttctgaa gggtcaagac
     9121 aagactgcgg ttcctggaaa atcatcgaca cgttgtgacc aaccagcttg cgccgttcgc
     9181 gtgcggagag acgcagcaaa tcgatatcat caaaacgcat acggtcagca gtaacacgcc
     9241 agttatcttt attcacccca caaatcgctt tcgcaatcaa acttttgccg gaaccggatt
     9301 cgccaacaag accgcggatt tcaccttcgg ttaaggtcat acttacgcgg tcgacggctt
     9361 taacccactc atcaccggtt ttaaattcaa tggtcaggtt acgaatatcg agtaatggca
     9421 ttattccacc cccgcaataa ttgcacgacg aacaccgtcg ccaaggaggt taaccaacag
     9481 cacgctaatc ataattgccg cacctggcag catgacagtc cacggggcga cataaatcag
     9541 ttccagcgca tcaccgagca tcgctcccca ttcaggcgag gggagttgtg cgccgagatc
     9601 gagaaagccc agcgcggcga tatcgagaat tgccatcgac agtgcgcggg tgatctcggt
     9661 gaccagcccg gcggtgatgt tgggcatcac agcaaaccag agaatattca gcgttgatgc
     9721 gccatccaga cgggcggcga taacgtactc tttttccagt tcgtcatgca ccatgctgta
     9781 aatcgaacgt accatacgcg gcagcaatgc cagccagaca gcaaacatgg cgtgagacaa
     9841 actcggtccg gcaaacgcta caacgataat tgccagcagc agcgaaggga tcgccagtaa
     9901 ggtatccaga atatggttaa gcaccgctga gcgtaggccg tgggtcgccc cggcgaaggt
     9961 gcccagaacc aggccacaga tcgtcgcggc aagcgtcacc acaaatgcgc cacctacggt
    10021 tggcgcagct ccgctcagta aacggcttaa aacatcgcgt ccgaggtcgt cagtccccag
    10081 gaagaaagaa acttcgccat agcgcgacca tgacggcggc aataattggt aaccgagaaa
    10141 ttgctggtcg atgccgtaag gcgcaaacca gccgccaaaa atacacagta cagccagccc
    10201 cgcgcagccg tacaggccga ccatcgcaga ggcgtcacta taaaatttgc gccaggcggt
    10261 acgcagcgtg cccggcgggc gcttttcgct gtatacgcta tcgtaaggca taccattcct
    10321 tatgtttcag agggttagcc atggcaccca aaatatcaga aatcacgtta acaataatga
    10381 ccagtgagcc acacaccatc actccggcgg aaatggctgc gtaatcctgc tggcggatgg
    10441 cgttaattaa ccagcgccct aaacccggcc agctaaaaac catttcggtg atcatcgcta
    10501 gcgtcagcat ggtagaaaac tgtaagccca ggcgaggaat aaccggaggt aacgcgttat
    10561 gcagaacgtg gcgacgcaaa atagtaaagc gcgacaaacc gcgggtcgcc gctgctttaa
    10621 catagttctg gtcatacact tcgatagtgc taatacgcat cagtcggatc acttcagttg
    10681 ttggcgcaac cgacagggtg atcacgggca atatcatatg gcggattgcg ctcatgacca
    10741 tttcatcccg ccatggtgag tccgaaagcc aggcatcaat caacgcaaaa ccggtaatcg
    10801 gtttcacttc gtagagcaga tcgaaacgcc ctgaaacggg caaccaacct agtgtgagtg
    10861 aacaaaacag cgtcagcaga agcgccagcc agaacaccgg gattgaaaag cccagtaagg
    10921 cgatggcgtt gatcaagtta tcctgccact tatggcgcgt aatcccggca atcattccta
    10981 cggggatgcc aacaatcagc gcaaaaccga aggcgaggat gcacagttcc atcgtcgccg
    11041 ggaagacctc ttttaactgt tcggcaatcg gttgaccgtt aatgctggaa acaccaaaat
    11101 cccagtggat caggccgtta aaccagaaca cccaggcatt ccacagtgac gcgccttgta
    11161 acggcgcatg tggggtgaaa tagctcaagc tgaagccaac gaaggtcagc aggaacaagg
    11221 tgacaatcaa taacaaaata cggcgcaagg tgaagataat catggctttt tcacctcatc
    11281 ctgtttctcg cgatacaccc cagcaaagga ggcgttacca aacgggctaa gtaccagacc
    11341 tttgatatcg taccgatagg cctgcaagcg caatgacgac gccagcggca gaatgggtaa
    11401 ttcctgcgcc agaatactct gcgcttcgtc ataggcttca atacgcgccg ccagctgctg
    11461 cgaggagagc gccttacgca acacgctgtc gaatttcgga tcgcaccagt gggcgaggtt
    11521 ggtctgtgaa tgaattgccg cgcagctcag taacggacgg aagaaactgt ccgggtcgtt
    11581 actgtccgtc gcccaaccgg ataacgtcag atcatggctc atatccatca accgcgcctc
    11641 ctgaaaacga ccttctaccg gcacaatcac cacttttacg ccaacctgtg ccatatccgc
    11701 ctgaatcagt tcggcggttt tcagcggact ggggttccac gcctgcgaac gtgttggcac
    11761 ccacagtttc agcgttaagt tttccagccc caacgccttc aactgttcgc gcgattttgc
    11821 cggattgtat tcagtaattt tagcctcgtt gtcataggcc cacgaggcgc gcggtaaaat
    11881 agaggccgcc gtttcagccg taccataata gatggattgc atcaggcgct ggttattaat
    11941 cgccagtgcc agcgcatggc ggacagcggg gttatttagc ggcggtttag cggtgttaaa
    12001 cgccagatag gcgacgttca tccccgggcg cagcgttaaa cgcaagcgtg ggtcgtcacg
    12061 caaaatggat agctgactgg cagcaggcca ggccagaacg tcgcactccc cggtcaggag
    12121 tttcgacaat cgtcctgtac cgccagagcc taaatccacc accacttgtg gcatcaacgg
    12181 cttaccacgc cagaaatcat catgacgttg taagcgaata tattgcccgg cgcggtattc
    12241 cgacaactga tacggtccag tgccgaccgg ctgacggtcg agttgctcct ggcgatcttc
    12301 tttctctaac ttccgggcat attctgccga catgaccgaa gcataatggg ttgcgaggtg
    12361 ccacaaaaaa gaagcatccg gctgagccag acggaactca acggtatgat tatccagttt
    12421 acggacgctt ttcacgttat cggcaaattg caggctgtcg aagtagggga agttgctgcc
    12481 gttgacgtta tgccacgggt tgttgcggtc aaaaattcgc tggaaggtaa acaccacatc
    12541 gtcggcattc attttacgag tgggagtaaa ccagtcggtt ttttgaaacg gaacatcgcg
    12601 acgcaggtgg aagcgatagg tcgcgccgtt gtcgagtact tcccagcttt cggcaagttc
    12661 cggcatcagg cgataggtat agggatcgac atccagcagt cgatcataaa actgggcggc
    12721 aagggtatcg acaattaacc cactgctcgc tttggatggg ttaaaggtgt tgacttgccc
    12781 gctgacgcaa tagacaaaac cgctgtcgcg gatatcagca tgcggggggg attcaggcgc
    12841 ggcgattgcc tgaccactca caagtccagc aatcaccaaa agagacgata atacctggcg
    12901 cataatatta atggatttta tgtaaagagg ctatcttact aatatttaat gacatttgcc
    12961 attaccgttt gtgttcaggg gtcgtaatga gggccgtgtg gttggtcgtt gaacagatga
    13021 cgtcgccatc tgttcaacat tcgtacccgt gatttctcta taactataac tcacagaaca
    13081 acttagcgag gagcagagcg gtacgatcgt gagaaaccca ttgaggcacg ctgcagtgcg
    13141 tgtcattgtt agccggatgc ggcgtgaacg ctttatccgg acaacgatac tgaccgagag
    13201 tctgcaatga aataattact cattacccca ttgattcaaa aactctgcga tctcatcaat
    13261 gcgtacggga ttaatccccg cttcagcagc catttcatgt tgggcttctt cgctgatctc
    13321 ttcattgttc atcaaacggg tgagcagtaa ctggaagtag agggccagcg gtgtgcgttc
    13381 agcgtctgcg acaggctctg cgcattcggt cgcgtactca tcggcaatat caaaatattt
    13441 tagcgggata tcgtggttca ttatttgccc ctggggttaa tgcgctaagc ataagggcgg
    13501 atgatagcac tcgtgatccg ggtcatacat ctttttatag ttaatggcgc ggtgcattac
    13561 cctcaggcag gataatgcgc cgcgcatccg actattacgt ttcgctcacc ggcgttctgt
    13621 caccatcata caacggcact ttgcgatagc ggcggatcag ataccacaaa tatgcgccgc
    13681 ctaacgaagc ccataccaga ccgagtgtca gtgacgttga ctcgaggtta acccacaaca
    13741 cacccaccgt cagcgcgcca accagcggca tcagcaaata gtggaagtga tccttccagc
    13801 ttttattcat tcctttacgc cgccagaaat gattaaacac cgacagattt acgaaggtaa
    13861 atgccaccag agcaccgaag ttaatcaatg ctgtcgcggt gactaaatca aagaacagcg
    13921 ctgacaacgc gacaatcccg accataatga cgttcagtgc cggagtccgc catttcgggt
    13981 gcacatagcc aaacacgcgc tccggaaaca cattgtcacg ccccatcaca tacagcagac
    14041 gcgacacgct ggcgtgcgac gccaggccag acgctaacgt gttcacaaac gtggtacaga
    14101 ggaaaatcga ctggaagagc ttgccgccga cgtatagcgc aatttcaggc agtgcggcgt
    14161 ccgggtcttt aaagcggctg atatcgggaa agaacagctg cataaagaac gacgcagcga
    14221 taaagataac gccgccgtat accgccgtca ggaagatggc tttcgggatc acgcgtgcgg
    14281 cgtccggcgt ctcttccgaa agcgtggtca ccgcatcgaa accgaggaag gagaaacaga
    14341 caatcgtcgc cccggtaata attgggatca ggtgcgcgtt ctcgctgata aacggctgaa
    14401 gtgaccagac ggtgccaacg ccttctcctt tatgcagtcc ctgaacaacc aggaagataa
    14461 acaccaccat gatggagatt tgcaccagta caaacaaggt attgaagtta gcgaccaggt
    14521 tgacgctctt cagattcgcg gcggttaaaa tggcaacgaa ggttaccacc cacacccacg
    14581 gcggcacttc cgggaagagg gcggagagat agattttcgc caacaaaacg ttgatcatcg
    14641 gcaaaaagag ataatccagc agcgatgacc agccgaccat aaatccgacg tgcgggttaa
    14701 tcgacttttg cgcgtaggta taggccgaac cggcctccgg aaactggcga accagtttgc
    14761 cgtagctgat agcggtaaac agcacgcccg ccagcgccag caaataggac gccggaacgt
    14821 gaccgtcgct aatgccggag acaataccaa aagtatcaaa tacggtcatc ggcgtgagat
    14881 aggccagacc catcatcacc acctgccaca atttcagtga ttttcgcaga cgggttttgc
    14941 caggttgcgc agcaatattc agtggtgaat taatagccat aattgctgcc tcccctgcgt
    15001 aataaggttt gcggtcgtgt cgacctgaaa tcaaacctgt ttagcaaggg aagcagaggg
    15061 ggcggagtcc agtcagtcgc aggaggggcg catacgcagc ctccctcgcc atgcggggat
    15121 ttaatcatcg gcatcatctc atttcctcgt cacagtctta atcgttaacg gaagcccgtt
    15181 gccgcctgcg ctccataacg cgacatccgg gaaatgtctg gattaccagc aaatgtcagg
    15241 cctggctccg ctcaggccga tgaaacaacc ccgcaagggg tattacgcgt ttttcaacat
    15301 ccactcaatt tctgtttctg tgatgaggcg ctcaaactgc aacagctcat catttttaca
    15361 ggcgtgatag acatggcaga agcgttcgcc taaatagcgg cgcaggtgat cattctcgat
    15421 aaactcaccc agggcatcgc tctggcgaat cgggaagggt aagccttcct gttccagccc
    15481 gttaccttcg acttcttcct gtaatggcag ttcgttatca aggccatgca aaataccggc
    15541 aaaaatcgcc gccatcacca gatacgggtt ggcatcggca cccgccacgc gatattccac
    15601 gcggtgatta tggcgatcgc cgcagggaat acgcagggcg acggtgcggt tgttatggcc
    15661 ccacgacgcc tgcgtcggta catacatccc cggctggaag cggcgatacg agttcacgtt
    15721 tggtgccagc aacgccattg atgacggcat caggtcaatc atcccggcga gcatcttttt
    15781 cagcagcggc gaatcttcgc cttccgcgtc agaaagcacg ttctcgccac gattattttg
    15841 catactgata tggatatgca tcccgctgcc cgcgtgctct tcatacggct tcgccataaa
    15901 agtggcgtgc atcttatgct tttctgccat cagacgcacc aggcgtttta gtgccagcgc
    15961 atcatcgcaa gcttccagca cgttatcggt atggtaaaga ttgatttcaa actgacctgg
    16021 cgaggcctcg gcgaccgcgc catctgccgg aatcagctgt aactgcgcca gctcatcaat
    16081 atcattgagc acgtcggcga agtggttgag gttatcaacg gagtaaacct ggctttgtgt
    16141 attgcggtca tcggtgccgg gcgcgcaggg cggttgcaga tatccttcag cgtcgcgctg
    16201 gcgatccagt aaatagaact ccagctctac cgctacgacc gggaacaatc cgcgctggcg
    16261 cagctgctgc cacaggcggt taagaacgtt ccgcggctca acgtcaaagg gagcgccatc
    16321 ttcatcgacc atggtcagga gcatctgacc gataaactct ggatcggcgg cagaaggggt
    16381 taaggaaccg agaacaggaa cacaggtacg atccggctcg cccatttcct gacccaggcc
    16441 cgcttcttct actacgttgc ccagaatatc cattgcaaac accgaggccg ggaaataaca
    16501 ccctttctcg agcttcttca ggcttgaaac aggaatacgt tttccacgga agcaaccatt
    16561 caaatcggta agtaaaacat caacatattg cgtattcggg tagcgctcca ggtagcgttt
    16621 cacttcctgc gtaaaggagc taccccgcct ctcttctgac tgctgaacaa agttctctac
    16681 ttcaacgata ttggtttcca tgattcttcg cctttggttt gttttccgct cgttatcaaa
    16741 gcgtaaaata taatgaccac tattcgaatc tgtatgcaaa ctaaatgttt gtcaaatgtt
    16801 aaattgagtt tgcaaaaatg aaaacccact gctagattga aaaaatattg aacataaagg
    16861 tcatttaaag cgcagtaatg gcgataattt agtccacttt gtgagattga gcatggaaaa
    16921 tataatgaac aatccggtta tcggtgtcgt aatgtgcagg aacaggctta agggtcatgc
    16981 gacccagact ctgcaagaaa agtacctgaa tgccatcatc cacgcaggcg gcttgccaat
    17041 tgcgctgcca catgcgctgg cggaaccgtc attacttgaa caacttttgc cgaaactcga
    17101 tggcatttat cttcctggta gtcccagcaa tgtgcagccg cacctatatg gtgaaaacgg
    17161 cgatgagcct gacgccgatc ctgggcgtga tcttctgagc atggcgttaa ttaatgccgc
    17221 actcgaaagg cgcatcccca ttttcgccat ctgccggggt ttacaggaac tggtggtggc
    17281 aaccggtggt tcgttgcatc gcaagctgtg cgaacagcct gaattgctgg aacatcggga
    17341 agatcccgaa ctgccggtgg agcagcaata tgcgccgtcg catgaagttc aggttgaaga
    17401 ggggggatta ctgtctgcgt tgttacctga atgtagcaac ttttgggtaa actctctgca
    17461 tggacaaggg gcgaaggtcg ttagcccacg gttgcgtgtt gaagctcgct cgccggatgg
    17521 cttagttgag gcggttagcg tcatcaatca tccttttgcg ctgggcgtac agtggcaccc
    17581 ggaatggaac agtagcgagt acgcgctttc gcgtatattg ttcgagggct ttatcaccgc
    17641 ttgtcagcac catatcgctg aaaaacagcg actctgacca ctacagttta aggaaatgca
    17701 aatatgagtg atgagggact ggcgccagga aaacgcttgt cggaaatccg ccagcagcag
    17761 gggctttcac aacgtcgtgc cgccgaactc tccgggctga ctcacagtgc tatcagtacg
    17821 atagaacaag ataaagtcag ccctgccatc agtacgctgc aaaagctgct gaaggtgtat
    17881 ggtctgtcac tctcggaatt cttttccgag ccggaaaaac ctgatgagcc gcaggtcgtc
    17941 attaatcagg acgacttaat tgagatgggc agtcagggtg tgtcaatgaa gctggttcat
    18001 aacggtaacc cgaatcgcac gctggcgatg atctttgaaa cttaccagcc gggcacaacc
    18061 actggggaaa gaattaagca tcagggtgag gaaataggca ctgtactgga aggtgaaatt
    18121 gttctgacga ttaatggtca ggattatcat ctcgtcgcgg ggcaaagtta tgccattaat
    18181 accggcatcc cgcacagttt cagtaatacg tcggcaggta tttgccgaat tatcagcgcc
    18241 catacgccca ccacgtttta atctttttgt tctgtaagcc gggtaagcgc aatgcgcccg
    18301 gcaatctata aagaatgtcg ccagaaataa acgggcatac ggcccgggga tctctgcgcc
    18361 ctgacgttca caaactgcat atatctgata gacgtgaaac aggagtcata atgaattttc
    18421 atcatctggc ttactggcag gataaagcgt taagtctcgc cattgaaacc cgcttattta
    18481 ttaacggtga atatactgct gcggcggaaa atgaaacttt tgaaaccgtt gatccggtca
    18541 cccaggcacc gctggcgaat attgcccgcg gcaagagcgt cgatattgac cgtgcggtga
    18601 gcgcagcacg cggcgtgttt gaacgtggcg actggtcact ctcttctccg gctaagcgta
    18661 aagcggtgct gaataaactc gccgatttaa tggaagccaa cgctgaagaa ctggcgctgc
    18721 tggaaacgct cgacaccggt aaaccgattc gtcacagcct gcgtgatgat attcccggcg
    18781 cggcgcgcgc cattcgctgg tacgccgaag cgatcgacaa agtgtatggc gaagtggcga
    18841 ccaccagtag ccatgagctg gcgatgatcg tgcgtgaacc ggtcggcgtg attgccgcca
    18901 tcgtgccgtg gaacttcccg ctgctgctga cttgctggaa actcggcccg gcgctggcgg
    18961 cgggaaacag cgtgattcta aaaccgtctg aaaaatcacc gctcagtgcg attcgtctcg
    19021 cggggctggc gaaagaagcg gggctgccgg atggtgtgtt gaacgtggtg acgggttttg
    19081 gtcatgaagc cgggcaggcg ctttcgcgcc ataacgatat cgatgccatt gcctttactg
    19141 gctcaacccg caccgggaaa cagctgctga aggacgcggg cgacagcaac atgaaacgcg
    19201 tctggctgga agcgggcggc aaaagcgcca acatcgtttt cgccgactgt ccggatttgc
    19261 aaaaagcggc gagcgcgacc gctgcgggca ttttctacaa ccaggggcag gtgtgtatcg
    19321 ccggaacgcg cctgttgctg gaagagagca tcgccgatga attcttagcc ctgttaaaac
    19381 agcaagcgca aaactggcag ccgggccatc cgcttgatcc cgcaaccacc atgggcacct
    19441 taatcgactg cgcccacgcc gactcggtcc atagctttat tcgggaaggc gaaagcaaag
    19501 ggcaactgct gctggatggc cgtaacgccg agctggctgc cgccatcggc ccgaccatct
    19561 ttgtggaggt agacccgaat gcgtccttaa gccgcgaaga gattttcggt ccggtgctgg
    19621 tggtcacgcg tttcacatca gaagaacagg cgctacagct tgccaacgac agccagtacg
    19681 gacttggcgc ggcggtatgg acgcgcgacc tctcccgcgc gcaccgcatg agtcgccgcc
    19741 tgaaagccgg ctccgtcttc gtcaataact acaacgacgg cgatatgacc gtgccgtttg
    19801 gcggctataa gcagagcggc aacgggcgcg acaaatccct gcatgccctt gaaaaattca
    19861 ccgaactgaa aaccatctgg ataagcctgg aggcctgaaa tgaccgaaca caccagcagt
    19921 tactacgccg ccagtgcgaa taaatacgcg ccattcgaca cgctgaatga gtcgatttcc
    19981 tgcgacgttt gcgtggtcgg cggcggctat accgggcttt cctccgcgct gcatctggcg
    20041 gaagcgggct ttgacgtggt ggttctcgaa gcctcacgca tcggctttgg cgcgagcgga
    20101 cgcaacggcg ggcagcttgt gaactcctac agccgcgaca tcgacgtgat cgaaaaaagc
    20161 tacggcatgg ataccgcccg tatgctcggc agcatgatgt tcgagggtgg cgagatcatc
    20221 cgcgaacgta tcaaacgtta tcagattgac tgcgactatc gccccggcgg tctgtttgtg
    20281 gcgatgaatg ataaacagct cgccacactc gaagagcaga aagagaactg ggaacgctac
    20341 ggcaataaac agctggagct gctggacgcc aacgctattc gccgcgaggt cgccagcgat
    20401 cgctacactg gtgcgctgct ggatcacagc ggtgggcata ttcacccgct aaacctcgcc
    20461 attggtgaag ccgatgccat ccgcctgaac ggcgggcgcg tgcatgagct ttccgcagtg
    20521 acgcagatcc agcacaccac gccagccgtg gtgcggactg ccaatggtca ggtgacggcg
    20581 aagtatgtga ttgtcgccgg gaatgcctac ctgggcgata aagtagagcc ggaactagcg
    20641 aaacgcagca tgccgtgcgg cacgcaggtg atcaccaccg aacggctgtc ggaagattta
    20701 gcccgttcgc tgatcccgaa aaactactgt gtggaagact gtaactatct gcttgattac
    20761 taccgtctta ccgccgataa ccgcctgctg tacggcggcg gcgtggtcta cggcgcacgc
    20821 gacccggacg atgttgagcg ccttgtggtg ccgaaactgc tgaaaacctt cccgcaactg
    20881 aagggcgtga aaattgatta ccgctggacg ggcaacttcc tgctgaccct gtcacgcatg
    20941 ccgcagtttg gtcgcctcga taccaacatc tactacatgc agggctacag cggccacggc
    21001 gtcacctgta cccacctcgc cgggcgtttg attgctgaac tgctgcgcgg tgacgccgag
    21061 cgtttcgacg ccttcgccaa tctgccgcat tacccgttcc ccggcgggcg cacgctgcgc
    21121 gttccgttta ccgcgatggg cgcggcttat tacagcctgc gcgatcgtct gggcgtttaa
    21181 tttccgatta acagtgaaga gtcaaaaggt gtgaaacatg agcaacaatg aattccatca
    21241 gcgtcgtctt tctgctaccc cacgcggggt tggcgtgatg tgtaacttct tcgcccagtc
    21301 ggcagaaaac gccacgctga aggatgtaga gggcaacgaa tatatcgatt tcgccgcagg
    21361 cattgcggta ctgaataccg ggcatcgcca ccctgatctg gtcgcggcgg tggaacagca
    21421 attgcaacag tttacccaca cggcgtatca gattgtgccg tacgaaagct acgtcacgct
    21481 ggcggagaaa attaacgccc ttgccccggt gagcgggcag gctaaaactg cgttcttcac
    21541 caccggtgcg gaagcggtgg agaacgcggt gaaaatcgcc cgcgcccata ccggacgccc
    21601 tggcgtgatt gcgtttagcg gcggcttcca cggtcgtaca tatatgacca tggcgctgac
    21661 cggaaaggtc gcgccgtaca aaatcggctt cggcccgttc cccggttcgg tatatcacgt
    21721 accttatccg tcagatttac acggcatttc aacgcaggac tccctcgacg ccatcgaacg
    21781 cttgtttaaa tcggacattg aagcgaagca ggtggcggcg attattttcg aaccggttca
    21841 gggcgaaggc ggtttcaacg ttgcgccaaa agagctggtt gccgccattc gccgcctgtg
    21901 cgacgagcac ggcattgtga tgattgccga tgaagtgcaa agcggctttg cgcgtaccgg
    21961 taaaatgttt gccatggatc actacgccga taagccggac ttaatgacga tggcgaaaag
    22021 cctcgcaggc gggatgccgc tttcgggcgt ggtcggtaac gcgaatatta tggacgcgcc
    22081 cgcgccgggc gggttgggtg gtacctacgc cgggaacccg ctggcggtgg ctgccgcgca
    22141 cgctgtgctc aacattatcg acaaagaatc actctgtgaa cgcgcgaatc aactgggcca
    22201 gcgcctgaca aacacgttga ttgatgccaa agaaagcgtt ccggccatcg cggcggtacg
    22261 cggtctgggg tctatgattg cggcagagtt taacgatccg caaacgggcg agccgtcagc
    22321 ggcgattgca cagaaaatcc agcaacgcgc gctggcgcag gggctgctct tgctgacctg
    22381 tggcgcatac ggcaacgtga ttcgcttcct gtatccgctg accatcccgg atgcgcaatt
    22441 cgatgaggca atgaaaattt tgcaggatgc gctgagcgat taatagccca acgcaataat
    22501 gtttgatgcg ctgcgcttat caggcctgca aacaacgtat tgattatgta tgccggataa
    22561 ggcgttcacg ccgcatccgg caagttgtat tgcccaactt cgctaaatct ggtgcttttt
    22621 caacaacgcg cggaactgat gataggttaa ccccagtaat tcagccgcgc gcttctggtt
    22681 atatttccct tgttgcaaac tgagttgcag caactctttt tcctgctgca tctgaaactc
    22741 acgtaaatcc agcggcagcg ttggaagcga ggtggtttct gaaacggcga tagcgtcttc
    22801 aggcggacgc cgtttaaagg gatcaataat gatgtcatca agcggataat cgctggtgcc
    22861 gtggcgatac accgaacgtt ccaccacgtt tttcaattca cgaatatttc ccggccagcg
    22921 ataattcagc aatgtttctc tggcgcgctc cgtaaaaccc gggaacagag gcagcttgat
    22981 ttcccgacac atctgaatgg caaagtgttc tgccatcagc ataatgtcgc tttcgcgctc
    23041 gcgcaggggc ggcagttgca ctacatcgaa agccagccgg tcgagcaggt cagcgcgaaa
    23101 agtaccttca ttgaccatcg ccgggagatc ggcattcgtc gcgcatacca accgtacatt
    23161 cacctgcaat ggctggctgc cgccaacgcg ctccagttca ccgtactcaa tcacgcgcaa
    23221 taatttctcc tgcaccatca tcggtgccgt agcgagttca tcaagaaata gcgtaccgcc
    23281 gtcggcacgt tcaaatctcc ctggatgacg tttttgcgca ccggtaaacg cccccgcttc
    23341 gtgaccaaac agttcggaat ccagcagatt ttcatttaac gccgcgcagt taagggaaat
    23401 aaacggcccc tgccaacggg aggagagata atgcagacgg ctggcaatca gctctttacc
    23461 tgtgccgcgc tcgccgatga tgagcaccgg tttatccagc ggtgcgagat gcgaaacctg
    23521 ttccagcact tcgagaaagc tgttcgcctc accgagtaaa ttatctttgt attctgccat
    23581 gatgaaattc gccacttgtt agtgtaattc gctaactcat cctggcatgt tgctgttgat
    23641 tcttcaatca gatctttata aatcaaaaag ataaaaaatt ggcacgcaaa ttgtattaat
    23701 agttcagcag gacaatcctg aacgcagaaa tcaagaggac aacattatgg gtattttttc
    23761 tcgctttgcc gacatcgtga atgccaacat caacgctctg ttagagaaag cggaagatcc
    23821 acagaaactg gtgcgtctga tgatccagga gatggaagat acactggttg aagtacgttc
    23881 tacttcggcg cgtgcgctgg cagaaaagaa acagctgact cgccgtattg aacaagcgtc
    23941 ggcgcgtgaa gttgaatggc aggaaaaagc cgaactggcg ctgctgaaag agagagaaga
    24001 cctggcgcgt gcggcgttaa ttgaaaaaca gaaactgacc gatctgatta agagcctgga
    24061 acatgaagtg acgctggtgg acgatacgct ggcacgcatg aagaaagaga tcggcgagct
    24121 ggaaaacaaa ttgagcgaaa cacgcgctcg ccagcaggca ttgatgttac gccatcaggc
    24181 ggcaaactcg tcgcgcgatg tgcgtcgtca gctggacagt ggcaaactgg atgaagcaat
    24241 ggctcgtttc gaatctttcg aacgtcgaat tgaccagatg gaagcggaag cagaaagcca
    24301 cagcttcggt aaacaaaaat cgctggacga tcagtttgcc gaactgaaag ccgatgatgc
    24361 aatcagcgag caactggcac aattaaaagc caaaatgaag caagacaatc aataataaca
    24421 tccaggcggc gtccgaacgc gccgccgctc atcgtctaag gagtccttat gagcgcgcta
    24481 tttctggcta ttccgttaac catttttgtg ctgtttgttt taccgatctg gttatggctg
    24541 cattacagca atcgttctgg tcgcagtgaa ttgtcgcaaa gtgagcagca gcgattagcg
    24601 caactggctg atgaagcaaa acggatgcgc gaacgtattc aggcgctgga atccattctt
    24661 gatgcagaac atccgaactg gagggatcgc taatggcggg cattaatctc aataaaaaat
    24721 tatggcgtat tccacagcag ggcatggtcc gtggcgtctg cgccgggatt gccaactatt
    24781 ttgatgtacc agtaaaactg gtgcgtatcc tggtggtgct gtcgattttc ttcggtctgg
    24841 cgctgtttac cctggttgct tacatcattt tgtcatttgc gcttgatcca atgccggaca
    24901 acatggcctt tggtgagcag ctaccttcca gcagtgaatt gctggatgaa gtcgaccgtg
    24961 aactggcggc aagtgaaacg cgtttacgcg agatggaacg ttatgtcact tccgatactt
    25021 tcacgttacg tagccgtttc cgtcaactgt gaggaaagtt atgaatactc gctggcaaca
    25081 ggccgggcaa aaggtgaagc ctggtttcaa attagcaggc aagctggtac ttcttaccgc
    25141 actgcgctat ggcccggcgg gtgtagctgg ctgggcgata aaatcagttg ctcgccgacc
    25201 actgaaaatg ttgctggctg tggcgctgga accgctgtta agtcgggctg ccaataaact
    25261 ggcgcagcgt tataaaaggt gaggggagtt tcgcaaaaat tgttaaagct aaggcgtatt
    25321 atggatggca attttcatcc atagaaggac gcttacatgt ttaaaaaagg cttacttgct
    25381 ctgacactgg tgttttcact gcccgttttc gccgctgaac actggatcga tgttcgtgtt
    25441 ccagagcagt atcagcaaga gcacgttcag ggagccatca atattcccct gaaggaagtg
    25501 aaagagcgca ttgccaccgc cgttccggat aaaaacgaca ccgtgaaagt gtattgcaat
    25561 gccggacgcc agtcagggca ggcaaaagag atccttagcg agatgggata tacccacgtt
    25621 gagaacgccg gcggcctgaa agacatcgca atgccgaagg tcaaaggtta aaagaatcca
    25681 ccctaagcgg cgcttatgcg ccgtttactt tttctgtcac accttattta catccccata
    25741 gattacttgc gtcagctcac aaatacgctt tttccctggt aaaaaatgat ttcctgcgtg
    25801 actaaaaccc ttgtgctcaa ttgacagttt attttctgcg gagtagtctc tcgtttcatg
    25861 ggaccgctac cacgaaaagg caacatggaa cagaaaatta cggattacct ggacgaaatc
    25921 tacggtggaa catttaccgc gactcattta cagaaacttg taacgcgtct tgagagtgcg
    25981 aaacgattaa ttacacagcg acgtaaaaaa cactgggatg aaagtgatgt cgtgttaatt
    26041 acctatgccg atcaatttca cagtaatgat ttaaaaccac tacccacatt taatcagttt
    26101 taccatcaat ggctgcaaag cactttttca catgttcatt tgttgccgtt ttatccatgg
    26161 tcatctgatg atggcttttc ggtaattgat tatcatcagg tcgccagtga agcgggggag
    26221 tggcaggata ttcagcaact cggtgaatgc agtcatttaa tgtttgattt tgtctgcaac
    26281 catatgtcgg caaaaagtga atggtttaaa aactatttac aacagcagcc aggttttgaa
    26341 gattttttta ttgcggttga tccgcaaacc gatctcagcg ccgtcacccg tccgcgtgcg
    26401 ttaccgttat taacgccatt ccagatgcgc gataattcaa cgcgtcattt atggaccacc
    26461 tttagcgacg atcaaattga cctcaattac cgtagccctg aagtgttact ggcgatggtg
    26521 gatgttttac tgtgttacct tgagaaaggt gctgagtata tccgtctgga tgccgttggc
    26581 tttatgtgga aagagccggg aacaagctgc atccatctgg aaaaaacaca tctgattatc
    26641 aaactgttac ggtcgattat tgataacgtt gcgccaggca cagtgatcat taccgagacc
    26701 aatgttccgc atatagacaa cattgcttac tttggcaacg gtgatgacga agcgcatatg
    26761 gtgtaccagt tctcgctgcc gccgctggtg ctgcatgcgg tgcaaaaaca gaacgttgag
    26821 gcactttgtg cgtgggcgca aaacctgaca ctaccttcca gcaacaccac ctggtttaac
    26881 ttccttgcct ctcacgatgg catcgggctt aacccactgc gggggttgtt gcccgaaaac
    26941 gaaatattag cgctggtcga ggtgttacag caggaagggg cattagtaaa ctggaaaaat
    27001 aatcccgacg gtacgcgtag cccctatgaa atgaatgtca cctatatgga tgcgttgagc
    27061 cgccgtgagt gtagcgacga agaacggtgt gccaggttta ttctcgccca tgcgattttg
    27121 ttaagtttcc ccggtgtgcc agcgatatat attcaaagca ttctggggtc gcgtaatgat
    27181 tacgcaggtg tcgaaaaact tggatataat cgtgcgatta accgtaaaaa atactctagt
    27241 aaagagataa cgacagaact gaacaataaa gcgacattaa ggtatttgat atattataag
    27301 ttgtcgcatt taattacact tcgtcgcagt cataaagagt ttcatccgga taataatttc
    27361 accattgatg cgattaattc atttgtaatg cgaattcaaa gaagcaatgc ggatggcgat
    27421 tgcctgacgg gattgtttaa tgtcagtgaa aatattcagc atataaatat tactgacctg
    27481 cacggtcggg atctgattag tgaagttgat atattgggta atgaaataac actgcatccc
    27541 tggcaggtta tgtggattaa ataaaaagga acatttcatg attaaatcaa aaatcgtgct
    27601 gttatcagca ctggtttcct gcgccctgat ttcaggctgt aaagaagaaa ataaaaataa
    27661 tgtatccatc gaatttatgc attcttcggt ggagcaggag cgccaggccg tcatcagcaa
    27721 attgattgcc cgttttgaaa aagaaaaccc aggtatcacc gtaaaacaag tgcccgtgga
    27781 agaagacgcc tataacacca aagtcattac cctttcacgt agcggttcgc tgccggaagt
    27841 gatcgaaacc agccatgact acgccaaagt gatggacaaa gagcagctta tcgatcgtaa
    27901 agcggttgcc acggtcatca gcaacgtcgg tgaaggcgcg ttctacgatg gcgtactgcg
    27961 gattgtgcgc accgaagatg gcagcgcctg gaccggtgtt cctgtcagcg catggattgg
    28021 cgggatctgg tatcgcaaag atgtgctggc aaaagctggg ctggaggagc caaaaaactg
    28081 gcagcaattg ctggacgttg cacagaaact gaatgacccg gcgaataaaa aatacggtat
    28141 tgcgctgcct acagcagaaa gcgtgttgac ggaacagtct ttctcccagt ttgcgttatc
    28201 caatcaggct aacgtcttta acgccgaagg caaaatcacc cttgatacac cagagatgat
    28261 gcaggcgctg acctattacc gcaaccttgc tgccaacacc atgccaggtt ctaacgacat
    28321 catggaagtg aaagacgctt ttatgaacgg caccgcgccg atggcgattt actccaccta
    28381 tatccttccg gctgtgatta aagaaggcga tccgaaaaac gttggtttcg ttgtacccac
    28441 cgagaaaaac tctgcggtct acggcatgtt gacctcgctg accattaccg tcgggcaaaa
    28501 ggccgaagag acggaagcgg ctgaaaaatt tgtcaccttt atggagcagg cagacaacat
    28561 tgccgactgg gtgatgatgt cgccaggcgc ggcgctgccg gtgaacaaag cggttgtcac
    28621 taccgccacc tggaaagaca acgacgttat taaggcgctg ggtgagctgc caaatcagtt
    28681 aatcagcgaa ctgccaaata ttcaggtctt tggcgcagta ggggataaaa attttacccg
    28741 catgggcgat gtgacgggtt ctggcgtggt gagctccatg gtgcataacg tcaccgtggg
    28801 taaagccgac ctccctggca cgctgcaaac gagccagaaa aagctggatg agttggttga
    28861 acagcgctaa acccaggaca ggaatccgca atgaacaggc ttttttcagg tcgttccgat
    28921 atgccctttg cgctgttgct tctcgcgccc agcttattac tgctgggcgg tctggtggcg
    28981 tggccgatgg tgtcgaatat cgaaatcagt tttttacgtc tgccgcttaa tcccaacatc
    29041 gagtcaacgt ttgttggggt gagcaactac gtgcgtattc tctccgatcc cggcttctgg
    29101 cactcgctgt ggatgacggt ctggtatacc gcgctggtgg tggcgggcag caccgtgctc
    29161 gggctggccg tggcgatgtt tttcaaccgc gagttccgcc tgcgcaaaac cgcgcgttcg
    29221 ctggtgatcc tctcctacgt aacgccgtct atttcgctgg tgttcgcctg gaaatacatg
    29281 ttcaacaacg gttacggcat tgtgaactac ctcggcgtcg atcttctgca tctctatgag
    29341 caggcaccgc tgtggttcga taatccgggc agtagctttg tgctggtggt gctgttcgcc
    29401 atctggcgct acttccccta tgcctttatt tcgtttctgg cgattttgca gaccatcgac
    29461 aaatcgctgt acgaagcggc agagatggat ggcgctaatg cctggcaacg gtttcgtatc
    29521 gtcacgctgc ccgcgattat gccggtcctg gcgacggtgg tgaccctgcg caccatctgg
    29581 atgttctaca tgttcgcgga tgtttatctg ctgacgacca aggtcgatat tctcggtgtc
    29641 tatctctaca aaaccgcctt tgcctttaat gacttaggga aagcggcggc gatctcggtg
    29701 gtgctcttca tcattatttt cgctgtcatt ctgctgacca gaaaacgggt gaacctcaat
    29761 ggcaacaaat aaacgcacac tcagtcgcat cggtttttac tgcgggctgg cgctgtttct
    29821 catcatcacg ctgtttccgt tttttgtgat gctgatgacc tcgttcaaga gcgcaaaaga
    29881 ggcgatctcg ctgcatccta cgctgctgcc gcaacagtgg acgctggagc attatgtcga
    29941 catttttaac ccgatgattt tcccgtttgt cgactacttc cgtaacagca tggtggtgtc
    30001 ggtggtttca tccgtggtgg cggtatttct cggcattctc ggggcttacg cgctttcccg
    30061 cctgcgcttt aaagggcgga tgaccatcaa cgccagcttt tacacggtgt atatgttctc
    30121 cggcattttg ctggtggtgc cgctgttcaa aatcatcacc gcgcttggca tatacgacac
    30181 cgagatggcg ctgatcatta ccatggtaac gcaaaccctg cccaccgccg tgtttatgct
    30241 gaaaagctac ttcgacacca tccctgatga gatcgaagaa gcggcgatga tggatggcct
    30301 caaccgtctg caaatcatct tccgtatcac cgtgcccctg gcgatgtccg ggctgatatc
    30361 cgtattcgtc tactgcttta tggtggcatg gaacgactat ctgtttgcgt cgattttcct
    30421 ctccagcgcc agcaatttca ccttaccagt cggcctgaac gcgctgttca gtacgcctga
    30481 ttacatctgg ggacggatga tggcggcctc gctggtgacc gcattgccgg tggtgattat
    30541 gtatgcgctt tcggagcgtt ttattaagag tggtttgacc gccggtggcg tgaagggcta
    30601 aagcggccag tttctttaca aggagtttta catgaaaaag ttagtagcca cagcaccgcg
    30661 cgtagctgcg ctggttgagt atgaagatcg ggcgatttta gccaatgaag tgaagatccg
    30721 cgtgcgcttc ggcgcaccga agcacgggac ggaagtggtc gatttccgcg ctgccagtcc
    30781 gtttatcgat gaagacttta acggcgaatg gcagatgttc accccgcgtc cggcagatgc
    30841 gccgcgcggc attgagtttg gcaaattcca gcttggcaac atggtggttg gcgacattat
    30901 cgagtgcggc agcgacgtta ccgactacgc ggtgggcgac agcgtatgcg gctacggccc
    30961 gctctccgag acggtcatca ttaacgcagt gaataactac aagctgcgca aaatgccgca
    31021 aggcagttcg tggaaaaacg ctgtctgcta cgacccggcg cagtttgcca tgagcggcgt
    31081 gcgcgatgcc aatgtgcgcg tgggcgattt tgtggtggtg gtagggcttg gcgcgatcgg
    31141 tcaaattgcc attcaactgg ctaaacgtgc tggcgcgtcg gtggtgattg gcgtcgatcc
    31201 tatcgcccat cgctgtgaga ttgcccgtcg ccatggcgcg gatttctgcc ttaaccccat
    31261 tggcagtgac gtaggcaaag agatcaaaac gctaaccggc aagcagggtg ccgatgtgat
    31321 tatcgaaacc agcggctatg ccgacgcgct gcaatcggcg ctacgaggtc tggcatatgg
    31381 cggcaccatc tcgtatgtcg cgtttgccaa accgtttgcc gaaggtttta acctcggacg
    31441 cgaagcccat ttcaataacg ccaaaattgt tttctcccgt gcgtgcagcg agccgaaccc
    31501 ggattatccg cgctggagcc gcaaacgtat cgaagaaacc tgttgggaac tgctgatgaa
    31561 cggttatctc aattgtgaag atttaatcga cccggtggtg acctttgcca atagcccgga
    31621 aagttacatg cagtatgtcg accagcatcc ggaacagagc atcaaaatgg gcgttacgtt
    31681 ttaattcaaa ggaatgaaaa aaatgaaaat cggcacacag aatcaggcgt tctttccgga
    31741 aaacattctg gagaaatttc gttatatcaa agagatgggt ttcgatggtt ttgagatcga
    31801 cggcaaactg ctggtcaaca atctcgaaga agtcaaagca gcaatcaaag aaaccggttt
    31861 accggtgatc accgcctgcg gtggctatga cggttggatt ggcgacttta tcgaagagcg
    31921 tcgacttaat ggcttaaagc agatcgaacg cattcttgaa gctctggcag aagtgggcgg
    31981 taaaggtatc gtcgttccgg ctgcatgggg catgtttacc ttccgcttac cgccgatgac
    32041 ctcgccgcgt agcctggacg gcgaccgcaa aatggtgagt gattccctgc gcgtactgga
    32101 aaaggtggcc gcacgtaccg gaaccgtggt gtacctcgaa ccgttaaacc gttatcagga
    32161 tcacatgatc aacactctcg ccgatgcccg ccgttatatc gtcgaaaacg atcttaaaca
    32221 tgtacagatt atcggcgatt tctatcacat gaatatcgaa gaagataacc tggcgcaggc
    32281 gctgcatgac aaccgcgacc tgctcggtca tgtgcatatt gccgataacc atcgctacca
    32341 gccgggcagc ggcaccctgg atttccacgc gctgttagaa cagctgcgcg cggataactt
    32401 tcagggctat gtggtgtacg aagggcgcat ccgggcggaa gatccagccc aggcgtaccg
    32461 tgattctttg gcctggttgc gtacctgcta agaggtcttt gtgaaaagtg caatgacaag
    32521 ctctccgctg cgggtcgcga taataggcgc gggccaggtg gcggataaag ttcatgcttc
    32581 gtactactgc acccgcaacg atctggaact ggtggctgtc tgtgacagcc gcctttccca
    32641 ggcgcaggcg ctggcagaaa aatacgggaa tgcatccgtg tgggacgatc cgcaggccat
    32701 gctgctggcg gtgaaacctg atgtggttag cgtctgctca cctaaccgtt ttcattacga
    32761 acataccctg atggcgctgg aagcgggctg ccatgtgatg tgcgaaaaac cgcccgccat
    32821 gacggcagaa caggcgcggg aaatgtgcga taccgcgcgc aaacagggca aggtgctggc
    32881 ctacgacttt caccatcgtt ttgcactcga tacgcaacag ctgcgtgaac aggtgaccaa
    32941 cggcgttttg ggagagattt acgttaccac cgcccgcgcc ctgcgtcgtt gcggtgttcc
    33001 cggctggggt gtctttacta ataaagaatt gcagggcgga ggcccgctga tcgacatcgg
    33061 cattcatatg ctggatgcgg cgatgtatgt gctgggtttt ccagcggtga aaagcgtgac
    33121 tgcgcatagc tttcaaaaga tcggcacgca aaagagctgc ggtcaatttg gcgagtggga
    33181 cccggcaacc tacagcgtcg aagattcgct gtttggcacc attgaatttc ataacggcgg
    33241 cattctgtgg cttgagacct catttgcact caacatccgc gaacagtcga ttatgaacgt
    33301 cagcttttgt ggtgataaag ccggggcgac gctgtttcca gcacatatct acaccgataa
    33361 caacggtgag ttaatgacgc tgatgcaacg ggaaatggca gacgacaacc gtcatctgcg
    33421 cagtatggaa gcctttatca accacgtaca aggtaagccc gtgatgatag ccgacgccga
    33481 gcagggatac atcatccagc aactggtggc ggcgctttat caatccgcag aaacagggac
    33541 gcgtgtggaa ttatgatcag gccagtaacg ttaacggaac cccatttcag ccagcatacc
    33601 ctgaacaagt atgcttcgct gatggcgcag gggaacggct atcttgggct tcgcgccagc
    33661 catgaagaag attacacgcg ccagacgcgc ggcatgtatc tggcagggct ttatcatcgg
    33721 gcgggtaaag gtgaaatcaa cgaactggtg aacctgcctg atgtcgtggg gatggagatt
    33781 gccataaatg gtgagatttt ctcgttatcc cgcgaagcat ggcagcgtga gcttaacttt
    33841 gccagtggcg aattacgccg caacgttgtc tggtgtacca gcaacggcgc aggttacacc
    33901 atcaccagcc gtcgctttgt ttcggcagac caactgccgc tcattgcgct ggaaatcact
    33961 attacgccac tggacgccga cgcgttagtg ctgatttcaa caggcattga cgccacgcaa
    34021 accaaccacg gtcgccaaca tctcgacgaa acccaggtgc gggtatttgg tcagcatctg
    34081 atgcagggga tctacaccac ccaggatggg cgcagtgatg tggccatcag ctgttgctgt
    34141 aaggtgagcg gtgatgtgca gcaatgctat accgccaaag agcgccgttt actgcaacat
    34201 acctgcgcgc agcttcacgc aggtgagacg ctgacgctgc aaaaacgggt gtggatcgac
    34261 tggcgggacg acagacaagc cgctttagac gaatggggca gtgcgtcgct tcgtcagctt
    34321 gaaatgtgcg cgcagcagag ttacgaccaa cttcttgcag catcaacaga aaactggcgt
    34381 caatggtggc agaaacgtcg tatcacggta aacggcggcg atgcgcacga tcagcaagcg
    34441 ttagattatg cgctttatca cctgcgcatc atgacgcctg cccacgatga gcgcagcagc
    34501 attgcggcga aaggtttaac cggcgaaggc tacaaaggcc acgttttctg ggatacagaa
    34561 gtatttttgc taccgttcca tctgtttagc gatccgacgg ttgcccgaag tttactgcgt
    34621 tatcgctggc acaacttgcc tggcgcgcag gagaaagcgc gacgcaacgg ctggcagggc
    34681 gctctgtttc cgtgggaaag cgcgcgcagc ggcgaagaag agacgccgga atttgccgcc
    34741 attaacatcc gcaccggact gcgccagaaa gtggcctcgg cgcaggctga acaccatttg
    34801 gtggctgata tcgcctgggc ggttattcaa tactggcagg caacggggga tgaaagtttc
    34861 attgcccacg aaggcatggc gttacttctg ggaaccgcaa aattctggat tagccgcgct
    34921 gtacaggtaa acgatcgtct ggaaattcac aatgttattg ggccagacga atataccgaa
    34981 catgtcaata ataacgcctt caccagctat atggcgtatt acaacgtcca gcaggcgctg
    35041 aatatcgctc gtaagtttgg ttgtagcgac gacgcgttta ttcatcgtgc cgaaatattc
    35101 ctcaaagaac tgcggctgcc agaaattcag cctgacggcg ttttgccgca ggatgattcg
    35161 tttatggcga aaccagcgat taatctggcg aaatacaaag cggcggcggg gaagcaaacc
    35221 attctgctgg attattcacg cgcagaagtg aacgagatgc agatcctcaa acaagctgat
    35281 gtggtgatgc tcaattacat gctgccggag cagtactcag cggcatcgtg tcttgccaat
    35341 ctgcaatttt atgaaccgcg caccattcac gactcttcac tgagtaaagc gatccacggc
    35401 attgttgccg cacgctgtgg cctgttggcg caaagttatc agttctggcg cgaggggact
    35461 gaaatcgatc ttggtgctga tccgcatagc tgtgatgacg gtatccacgc agccgcaact
    35521 ggcgctattt ggctgggggc gattcagggt tttgccgggg tgagcgtgcg taacggtgaa
    35581 ttgcatctca atccagcgtt acctgagcag tggcaacagt tgtctttccc tctgttctgg
    35641 cagggctgcg aattacaggt cactctcgac gcgcagcgta ttgcgattcg aacttctgcg
    35701 cccgtttcac tgcgtttgaa cgggcagctt atatctgtgg ctgaagaatc tgttttctgt
    35761 ttgggtgatt ttattttgcc cttcaatggg accgctacca cgcatcagga ggatgaatga
    35821 aactgcaagg ggtaattttc gatctggatg gtgtaatcac cgataccgcg catctgcatt
    35881 tcaaggcgtg gcagcagatt gccgctgaaa ttggcatcag cattgatgcg cagtttaacg
    35941 aatccctaaa agggatcagc cgcgatgagt ctctgcggcg cattctgcaa cacgggggca
    36001 aagagggcga ctttaacccg caggagaggg cgcaactggc gtatcgcaaa aatctgctct
    36061 acgtccactc actgcgcgaa ctgacggtta atgctgtgct gcccggcatt cgcaatttgc
    36121 tggcagatct ccgtgcacag cagatcccta tagggctggc ttctgtctcc ctgaatgcgc
    36181 cgacgatttt agcagcgctg gagctgcgcg agtttttcac cttctgcgcg gacgcttccc
    36241 aacttaaaaa ctcgaaaccg gacccggaaa tctttctcgc cgcctgtgcc ggactgggcg
    36301 tgccgccgca ggcatgtatc ggcattgaag atgcgcaggc gggcattgac gccattaacg
    36361 ccagcggtat gcgctcggtg gggatcggcg cgggcttaac cggggcgcaa ttattgttgc
    36421 cctcaacgga ctccctgacc tggccgcggt tatcggcctt ctggcaaaac gtatagcaaa
    36481 ggaatcaaca tggctcagct ttcgttacaa catattcaaa aaatctacga taaccaggtg
    36541 catgtggtga aggacttcaa cctggaaatt gccgataaag agttcattgt ctttgtcggt
    36601 ccgtcaggct gcggtaaatc gactacgctg cgcatgattg ccgggcttga ggagatcagc
    36661 ggcggcgatc tgttgatcga cggcaaacga atgaatgacg ttccggctaa agagcgcaat
    36721 atcgcgatgg tgttcctgaa ctacgcgctg tatccgcata tgacggtcta cgacaacatg
    36781 gcgtttggcc tgaagatgca aaaaatcgcc aacgaggtga ttgatgagcg ggtgaactgg
    36841 gcggcgcaaa ttctcggcct gcgtgagtac ctgaaacgca agccgggggc gctttccggc
    36901 gggcaacgtc agcgagtggc gctcgggcgg gcgattgtgc gcgaagcggg cgtgttttta
    36961 atggatgaac cgctctctaa cctggatgcc aagctgcgcg tgcaaatgcg cgcagagatc
    37021 agcaagctgc atcagaaact gaacaccacc atgatctacg tgacccacga tcagaccgaa
    37081 gcgatgacca tggcgacgcg gattgtgatt atgaaggatg ggattgttca gcaagtaggt
    37141 gcgccgaaaa cggtttataa ccaaccggcg aatatgtttg ttgccggatt tataggatca
    37201 ccggcgatga attttattcg cggcacgatt gatggcgata aattcgttac ggaaacgctt
    37261 aaattaacca tttccgagga gaaattagcg gttctgaaaa cacaggaaag tttgcataag
    37321 cccattgtga tgggaatacg cccggaagat attcatccgg acgcgcaaga ggaaaataac
    37381 atttccgcca aaattagcgt ggcagaatta accggtgctg aatttatgct ctacaccacg
    37441 gttggggggc acgaactggt ggtccgtgct ggtgcgttaa atgattatca tgcaggagaa
    37501 aatatcacta ttcattttga tatgactaaa tgtcatttct ttgatgcaga aacggaaata
    37561 gcaattcgct aaatacaggg ggaaggcatt cccccaggat aatacaagga acaataatga
    37621 aaaaattatt accctgtacc gcactggtga tgtgtgcggg aatggcctgc gcacaggccg
    37681 aggaaaagaa cgactggcac tttaatatcg gcgcgatgta cgaaatagaa aacgtcgagg
    37741 gttatggcga agatatggat gggctggcgg agccttctgt ctattttaat gccgccaacg
    37801 ggccgtggag aatttctctg gcctattatc aggaagggcc ggtagattat agcgcgggta
    37861 aacgtggaac gtggtttgat cgcccggagc tggaggtaca ttatcagttc ctcgaaagcg
    37921 atgatttcag tttcggtctg actggtggtt tccgtaatta tggttatcac tacgttgatg
    37981 aaccgggtaa agacacggcg aatatgcagc gctggaaaat tgcgccagac tgggatgtga
    38041 aactgactga cgatttacgt ttcaacggtt ggctgtcgat gtataaattt gccaacgatc
    38101 tgaatacgac cggttatgct gatacccgtg tcgaaacgga aacgggtctg caatatacct
    38161 tcaacgaaac ggttgccttg cgagtgaact attatctcga gcgcggtttc aatatggatg
    38221 atagccgcaa taacggtgag ttttccacgc aagaaattcg cgcctatttg ccgctgacgc
    38281 tcggcaacca ctcggtgacg ccgtatacgc gcattgggct ggatcgctgg agtaactggg
    38341 actggcagga tgatattgaa cgtgaaggcc atgattttaa ccgtgtgggg ttattttacg
    38401 gttatgattt ccagaatggt ctttccgttt cgctggaata cgcgtttgag tggcaggatc
    38461 acgacgaagg cgacagcgat aaattccatt atgcaggtgt cggcgtaaat tactcgttct
    38521 gataatgggt taaattgccg gatgcggcgc gagcgccaaa tccggcatac atttatttca
    38581 acgacatatg cgtggcaacg gtaatatttt gtggtgacgg ttttccggca attaagcgga
    38641 ataataactc gcagctttgt tgccctaact cctgcgtcgg aacatcgatg ccgcccggtg
    38701 caggcgttaa aataaatgac agcgtttcat tgctatagcc caccaccgct aactgctgcg
    38761 gaatagcaat atttttctct gctgccgcac gataaatgct cattaatttc aggctgtcag
    38821 tggcaaatac ggcttctggc aacggcgact ggcttaataa ttgccgtgcg gcttgcaagg
    38881 ctgtttcatg ggtataaccg ccgtcaacaa tccattcatc acgcactgca atattatgcg
    38941 cagccagact ttgcttatag ccattaacgc ggtcaaccga gacatggaca tcaagcggag
    39001 catgcaggca ggcaatattt tgattcccac tttcaattaa cgcatcggtc aacgcaatgc
    39061 tgtcgccaaa attatcggta tcgacagaat aaacatgggc atattgacct tcaactttgc
    39121 caattaccac caccggaata tcatatttat cgagttgggc aaaaaatgac tcatccgctg
    39181 gcgaacttag cataataatg cctttaatca ttttctgctt aattttgctt tcgcattttt
    39241 gtaagtcttc tgccaggttg tgcgacgtct gcaatatcac gtcgaaacct tcttcttcag
    39301 ctttggcggt gatggcatgt aaaacttccg agaaaaacgg attacccgcc gtagttttgg
    39361 tcgaacgggt agaaatcacc ataatggcat caaaacccga agaggtcagc gcgcgtgcca
    39421 gcttgtttgg ttgatactgt aattcttcaa tggcccgtaa cactttttcg cgcgcttccg
    39481 gggagatatt ggtttgctta ttcagcacgc gtgatacggt ggattttgat acgcctgcaa
    39541 ccctggcaat atcataaata gtaggggaca taggtcagga actccgtccg aacgtgaatg
    39601 ttgcacatct tatggagttc gcccgtcgat gacaacacgg gcagttgata atcaatggcc
    39661 tggcccccac attcatatcc ttacgaatga tttttttctg gtcttcagag cagcacagga
    39721 cagcaatgaa gcgacttaaa aatgaactta atgcgctggt gaatcggggt gtcgacagac
    39781 atctgcgcct cgctgtaacc ggacttagcc gcagcggcaa aacagcgttt atcaccgcga
    39841 tggttaatca gttgctcaat attcatgccg gagcacgttt gccgctatta agtgcggtgc
    39901 gtgaagaacg cctgctgggc gtaaaacgca ttccccagcg tgactttggc attccgcgct
    39961 tcacctatga tgaaggactg gcgcagttat atggcgatcc tcccgcctgg ccgacgccaa
    40021 cgcgcggcgt cagcgaaatt cgcctggcgc tacgctataa atcgaacgat tcgctgctgc
    40081 gccactttaa ggatacctcc acgctgtatt tggagattgt ggattacccc ggcgaatggt
    40141 tgctcgacct gccgatgctg gcgcaggact atttaagctg gtcgcgccag atgacgggct
    40201 tactcaatgg tcagcgcggc gaatggtcgg cgaaatggcg aatgatgtgc gaagggctgg
    40261 acccgctagc gcctgccgac gaaaaccggc tggcagacat tgccgccgcg tggaccgatt
    40321 atctccacca ctgtaaacag caggggctgc actttattca gcctgggcgc tttgtcttgc
    40381 cgggagatat ggcaggtgcg cccgcgctgc aattcttccc atggccagat gtcgatgcct
    40441 ggggcgagtc taaactggcg caggccgata aacacaccaa cgccggaatg ttgcgcgagc
    40501 gttttaatta ttactgcgag aaggtggtga aaggattcta taaaaatcac ttcctgcgtt
    40561 ttgaccgcca gattgtgctg gtggactgcc tgcaaccgct caacagtgga ccacaggcat
    40621 ttaatgatat gcgtctggcg ctgacgcagc tgatgcaaag tttccactac gggcagcgta
    40681 ccctgttccg gcgtttgttt tcgccggtta tcgataagct attgtttgct gccactaaag
    40741 cggaccatgt gaccatcgat cagcacgcta atatggtttc attactgcaa caactgattc
    40801 aggatgcctg gcaaaatgca gcgttcgaag ggatcagcat ggactgcctg gggctggcgt
    40861 cagttcaggc gaccaccagc ggcattattg atgttaacgg tgagaaaatc ccggcgctgc
    40921 gcggtaatcg acttagtgat ggcgcaccgc tcactgttta tcctggcgaa gtccccgcac
    40981 gtttgcctgg tcaggcgttc tgggataagc aaggcttcca gtttgaggcg tttcgtccgc
    41041 aggtgatgga tgtcgacaaa ccgctaccgc atattcgcct tgatgctgcg ctggaatttt
    41101 taataggaga taaattgcga tgaccgaacc gttaaaacca cgtattgatt tcgacggtcc
    41161 actggaggtc gatcagaatc caaaattcag ggcgcagcag acctttgacg aaaatcaggc
    41221 gcaaaatttt gccccggcca cgctcgacga agcgcaggaa gaagaggggc aagttgaagc
    41281 ggtgatggac gcagcgttac gtccgaaacg cagcctgtgg cgcaaaatgg tgatgggcgg
    41341 gttggctctg tttggcgcaa gcgttgtcgg gcagggtgta cagtggacaa tgaacgcctg
    41401 gcaaacccag gactgggtgg cgctgggtgg atgtgccgct ggggcattga ttatcggcgc
    41461 tggcgtaggt tctgtggtaa cagagtggcg gcgcttatgg cgcttgcgac agcgcgccca
    41521 tgaacgcgac gaagcgcgtg atttattgca tagtcacggc acgggtaaag gccgcgcatt
    41581 ttgcgaaaaa ctggcacagc aggcgggcat cgatcagtca catccggcgc tgcaacgctg
    41641 gtatgcctca atccatgaaa cgcaaaacga ccgtgaagtg gtcagtttgt atgcgcattt
    41701 ggtccagcca gttttagatg cccaggcgcg gcgcgaaatc agccgttcgg cagcggaatc
    41761 aacattgatg attgcggtca gcccactggc gctggtggat atggctttta tcgcctggcg
    41821 caatctgcgt ttgatcaatc gcatcgccac gctgtatggc attgaactgg gatattacag
    41881 ccgtttgcgc ctgtttaagc tggtattgct gaatatcgct ttcgccggag ccagcgaact
    41941 ggtgcgcgaa gtggggatgg actggatgtc gcaagatctc gctgcgcgtt tgtctacccg
    42001 ggcagctcag gggattggtg ctggacttct gacggcacga ctggggatta aagctatgga
    42061 actttgccgc ccgctgccgt ggattgaaga tgacaaacct cgcctcgggg atttccgtcg
    42121 tcagcttatc ggtcaggtga aagagacgct gcaaaagggc aaaacgccca gcgaaaaata
    42181 atgcaatatc gggtgctgac cggatatctt tacgccgaag tgcccgtttt tccgtctttg
    42241 tgtcaatcat tgttgacaga aaccttcctg ctatccaaat agtgtcatat catcatatta
    42301 attgttcttt tttcaggtga aggttcccat gcgtctggaa gtcttttgtg aagaccgact
    42361 tggtctgacc cgcgaattac tcgatctact ggtgctgaga ggcattgatt tacgcggtat
    42421 tgagattgac cccattgggc gaatctacct caattttgcc gaactggaat ttgaaagttt
    42481 cagcagtctg atggccgaaa tacgccgtat tgcaggtgtt accgatgtgc gcactgttcc
    42541 gtggatgcct tccgaacgtg agcatctggc gttgagcgcg ttactggagg cgttgcctga
    42601 gcctgtgctt tctgttgata tgaaaagcaa agtggatatg gcgaacccgg cgagctgtca
    42661 gctttttggg caaaaattgg atcgcctgcg caaccatacc gccgcgcaat taattaacgg
    42721 ctttaatttt ttacgctggc tggaaagcga accgcaagac tcgcataacg agcatgttgt
    42781 tattaatggg cagaatttcc tgatggagat tacgcctgtt tatcttcagg atgaaaatga
    42841 tcaacatgtt ctgaccggtg cggtggtgat gttgcgctcg acgattcgta tgggccgtca
    42901 gttgcagaat gtcgctgcac aggacgtcag tgccttcagt caaattgtcg ccgtcagccc
    42961 gaaaatgaag catgttgtcg aacaggcgca gaaactggcg atgctaagcg cgccgttgct
    43021 gattacgggt gacaccggta caggtaaaga tctctttgcc tacgcctgcc atcaggcaag
    43081 cccacgagcg ggcaaacctt acctggcgct gaactgcgcg tctataccgg aagatgcggt
    43141 cgagagtgaa ctgtttggtc atgctccgga aggtaaaaaa gggttctttg agcaggcgaa
    43201 cggtggttcg gtgctgttag atgagatagg cgagatgtcg ccaaggatgc aggcgaaatt
    43261 actgcgtttc ctcaatgatg gtactttccg tcgagttggt gaagatcatg aagtgcatgt
    43321 cgatgtaagg gtgatttgcg ctacgcagaa gaatttggtc gaactggtgc aaaaaggcgt
    43381 gttccgtgaa gatctctatt atcgtctgaa cgtgttgacg ctcaatctgc cgccgctacg
    43441 tgactgtccg caggacatca tgccgttaac cgagctgttc gtcgcccgct ttgccgacga
    43501 gcagggcgtg ccgcgtccga aactggccgc tgacctgaat actgtactta cacgttatgc
    43561 gtggccggga aatgtgcggc agttaaagaa cgctatctat cgcgcactga cacaactgga
    43621 cggttatgag ctgcgtccac aggatatttt gttgccggat tatgacgccg caacggtagc
    43681 cgtgggcgaa gatgcgatgg aaggttcgct ggacgaaatc accagtcgtt ttgaacgctc
    43741 ggtattaacc cagctttatc gcaattatcc cagcacgcgc aaactggcaa aacgtctcgg
    43801 cgtttcacat accgcgattg ccaataagtt gcgggaatac ggtctgagtc agaagaagaa
    43861 cgaagagtaa gcgcgaatat gcctgatggt gcaacaccat caggcatatt aacttatgct
    43921 ttcagtacag ccagagctgc ttcgtaatcc ggctcggtgg tgatttcatc caccagctgg
    43981 ctgaaaatca cattgtcatt ttcatcaata accacaacgg cacgcgctgc cagacctttc
    44041 agtgggccat cagcaattgc cacaccgtag gcttgcagaa attcagcgtt acggaaagtg
    44101 gagagggtga taacgttgtt cagaccttct gcgccgcaga aacgagactg ggcgaacggc
    44161 agatcggcag agatacacag cacaacggtg ttgtcgatct ctgttgccag ttggttaaac
    44221 ttacgtactg atgcggcgca aacaccggta tcaatactcg ggaaaatgtt cagcactttg
    44281 cgtttacccg caaactgacc gagggtgacg tcagacagat cttttgccac gagagtaaaa
    44341 gtctgcgctt tgctacccgc ctgcgggatg gaattggcga ctgtaaccgg gttgccctgg
    44401 aaatgaacgg tttgtgacat gattatcttt cctgtttaca tatagttaac gtcacaccta
    44461 gtttatgcta actgtcaata acacagcaaa cgctatttgc gcttaatccg cagaccaccg
    44521 cgacaacaag gagtaaagat gagaaccgtt aaggtattcg aggaaggctg gcccttacat
    44581 accccgtttg tgattgctcg gggaagtcgc agtgaagcgc gcgtggtggt ggttgaactg
    44641 gaagaagaag gtattaaagg caccggtgaa tgcacgccgt atccgcgtta tggagaaagt
    44701 gatgcctcgg taatggcgca aattatgagc gtcgtgccgc aattagagaa agggctgaca
    44761 cgggaggagt tgcaaaaaat cctccctgcc ggcgcagcac gtaatgcgct ggattgtgca
    44821 ttgtgggatc tcgcggcgcg aaaacagcag gaatcgctgg ctgatttgat cggcataacg
    44881 cttcccggga cagttatcac tgcacagacg gttgtcatcg gtacgcctga tcagatggcc
    44941 aatagtgcat caacactctg gcaggcaggc gcgaaattac tgaaagtgaa gctggataac
    45001 catcttatca gtgagcggat ggtggcaatt cgcaccgctg tgcccgatgc gacgctgatc
    45061 gttgatgcaa atgaatcctg gcgtgcagaa gggttggcgg cgcgttgcca gctattggcg
    45121 gatttaggcg ttgcaatgct tgaacaaccg cttcctgcgc aggacgatgc ggcgttggag
    45181 aattttattc atccgttacc gatttgtgct gatgaaagtt gtcatactcg tagcaatctg
    45241 aaggcgctga aagggcgcta tgagatggtt aacattaagc tcgataaaac ggggggcctg
    45301 acggaagcgc tggcgctggc gactgaagca cgtgcacaag gttttagtct gatgctgggc
    45361 tgcatgttgt gtacctctcg tgccattagc gccgctttac cgctggtgcc gcaggtcagt
    45421 ttcgccgatc ttgacggacc gacctggctg gcggtagatg tggaaccggc gcttcagttc
    45481 acaacgggcg aattgcatct ttaggatgcc agcgcagcaa gtttgccatc gcaaagagat
    45541 atttttcgct ggcttcgtcg gaggagatgg gtggaaactc tgcggtgatg caatgcaggt
    45601 tgagatccgc acaccaactg ccgaaagagc caggcgtttc ataaccgacg ctggttacca
    45661 gaggcaattc aaacgcctgg gccagccatt cacctaattc gctgtgtctg ggatcttcaa
    45721 tacaggccag cggatcgtgg aatgagacca cccaggcggg ttgaatgcgg tgtataagct
    45781 ggcacagcgc ctgggtttca ggttcggagc caggtttgtc acctgtcagt aaaacgacat
    45841 cacgttcttc agcggcgctg ttccagcgat aaacggtttc accttccttc cagtttgccg
    45901 ccggaaagtt tctgtttaaa tccacaccat tagcattggc ccttaacccc aactggcagc
    45961 cgtcaggatt cacacacagc accacatgat gacggcgcaa agaaggtgtc aacgtccgca
    46021 gagcacagga gagcgtgacg atcgaagagt tttcatcacc gtgagtgcca gccagaatca
    46081 aaccactttc gtgactggct gcaggggccg gaaaccagat taacggcgcg cccaataatg
    46141 aacgtccgta atgttctgtt ccgggcggaa atgcgccgcg ttcggcgcgt gggcgagtta
    46201 cggtcatgat cgtctctggt tcaggaagta ttacaggcag tgttgtgcaa attttgcgga
    46261 ttatcaaatc cttttcttca acgctatgtt atcgatcttg tatcactttt ttgccggaag
    46321 ttgtttgcat tttcattgat gtgcagatat tagaattgtc gtaatgttat atacatggcg
    46381 tattttattc ttactggttc tacacatgtt aatcgcatct gattgatcag ttcaccttaa
    46441 taagtttaat aaatcgtact gcacatagta cagtacgatg gtagctatat aattgattta
    46501 catgaagaat ggcttatcgc catcagtccc gggttgattg attcctatat tctcaccaat
    46561 gtgtttctca ggtttatcga tgatatctgt aattaatgct gcgacactct tacgtgatac
    46621 gatcgtacct ttgaatggtt cattgcgtga ggtaagctca taatcaataa tatcttcatc
    46681 agtcagccag gcagggcgaa gaattgtata ttccagtcca gaagcctcaa ttgcatctgc
    46741 agccctgcgg aatggtttta ggggttcacc aataactgca ttattccatt cgccaaattt
    46801 tcctggaact tcatcataaa taccgaggga aagaacaaag atcagacgtt taacatcacc
    46861 ggctttcatc gcagcgatca ctgaatttgc ctgaatatct aaatcttccc ccgtaaggtt
    46921 ggcatacaca acatcctgtc cctgcatggc ctgcttcagt gctgcatgat tcagtacatc
    46981 ccccataata atcttgctat tagtgggata gggtttgtgg atttttgctg gctgacgggc
    47041 aaataaagtt tgtttaatgg tttgtttatc tgccagttga ttaatcacat ggcgggctat
    47101 ctgaccgcca gcaccaagaa tcagtacatt tttcatgaaa cttttcccag attattggca
    47161 gcaatatgcc aggccatatc actattacgg atttgaatat gtttcattac agagtttcct
    47221 caaagaacgg agctaatact gaaattgctt cagccacata gactttgccg tcataaagat
    47281 ccatatggtt tgcgccttca acaatgtgat agcgtttatc ctggcttgat gctcgatcgt
    47341 acaggtcgtc actcatccat ttgctccctg cctggctgcc cgccacaatc tgtatcggct
    47401 gagtcaggta cacttccgcc atatggtaag catcataggt aataatctgg ttaaggctgc
    47461 gcaaagtagc gtaacccggt gctgttggat actgcgcgcg aggggtatgg taatactccc
    47521 aggcctgacg cagttcttcg ttcggcgcat cggactcctt cattggtgcc agtggcatag
    47581 tggcgtattc tctgctgcta atatcgctgg ttcgggcgtt tgaacccgcg tcaacgtatg
    47641 gtagggcatc tatagatttc acattgtttt cccaaccatt acggaacatc gaaccaatat
    47701 tgaccgcact aacagtaccg atggccttga tgcggcgatc ctgaattgca gcattggctg
    47761 tatatcctgc accggcacaa attcccatcg caccaattcg gctattgtcg acataagaaa
    47821 gcgttgtcag gtaatcaatc acggcactga cgtcttcagt acgaatgtat gggttttcta
    47881 actgacgcgg ctcgccgccg ctttcacctt gataagatgc gtcataagca atagtgacaa
    47941 atcccttttc cgccagtttt tcggcatagg ttccagccgt ttgctcttta acgcccccac
    48001 ctgggtgaga taccacaatt gccggatact gacgggtttc atcaaatttt ggagggaaat
    48061 agatcactgc agacaaagag atggttggat tattgctgtt agtgaagctg actttattat
    48121 tcatcattcc gttcctcatg gagttgtcgg ttcgttttga cggttggtga tatcactata
    48181 gatattgatc attaagttga ttagacccaa aatcatgatt agacctatca tttaaatgat
    48241 taatagattg tatggagata ctgaatgaag cgtgaagaaa tcgctgatct gatggcgttt
    48301 gtcgtcgttg cagaggagcg tagcttcact cgcgcagcag cccgcctgag catggcgcag
    48361 tcagctttaa gccagatagt gcgccgtata gaagaacgat tgggattgcg gcttctgaca
    48421 cgaaccacgc gcagcgttgt acccactgaa gcgggcgagc atcttttgtc tgttcttggc
    48481 cctatgttgc atgacataga ttctgccctg acatccctga gcgatctgca gaaccgccca
    48541 tccgggacaa tacgtattac tactgtagaa catgcagcaa aaacgatatt gttaccagct
    48601 atgcgcacat tcctgaaatc gcatcctgaa attgatatcc agcttaccat tgattatggt
    48661 ttgaccgatg tcgtttctga acgttttgat gcaggcgtcc gtctgggtgg ggagatggat
    48721 aaagatatga tcgccattcg aatcgggcca gatataccaa tggctattgt tggctcaccg
    48781 gattattttt ctcgtcgaag tgctccaacc tcagtgtcac aattaataga tcatcaggca
    48841 attaatttgt atcttcccac atcgggtaca gcaaatcgct ggagattaat acgcggtgga
    48901 cgtgaagttc gtgttcgcat ggaaggtcag cttttactga atacgataga cctgatcatt
    48961 gatgctgcaa ttgatgggca tggattggcg tatctacctt atgatcaggt tgagcgggct
    49021 attaaagaaa aaaaactgat acgtgttttg gataaattca caccagattt acccggttat
    49081 cacctgtact atccacaccg tcgacatgct ggctcggcat tctcattatt tatagatagg
    49141 ctgaagtata aaggtgctgt ttagcactac ttgctgatac attaatttaa ttcttctctt
    49201 aacgtattct cagttccttt caacgttttg gttatttttt attcttcgta caatggcgac
    49261 agatgctgat tatgataccg aaaacgggtt tgaacgtgcg aagcccgaac gtagtgttcg
    49321 gagttctatg tgctttaccg cattttggag acta
//
LOCUS       DAZGHF010000018        49093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGHF010000018 DAZGHF010000000
VERSION     DAZGHF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.018"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            434..523
                     /gene="tisB"
                     /locus_tag="ACHK3A_001173"
     CDS             434..523
                     /gene="tisB"
                     /locus_tag="ACHK3A_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO8739287.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(647..721)
                     /gene="ysdE"
                     /locus_tag="ACHK3A_001174"
     CDS             complement(647..721)
                     /gene="ysdE"
                     /locus_tag="ACHK3A_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO8739288.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            803..1987
                     /gene="emrD"
                     /locus_tag="ACHK3A_001175"
     CDS             803..1987
                     /gene="emrD"
                     /locus_tag="ACHK3A_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO8739289.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(1995..2492)
                     /gene="yidF"
                     /locus_tag="ACHK3A_001176"
     CDS             complement(1995..2492)
                     /gene="yidF"
                     /locus_tag="ACHK3A_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO8739290.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(2489..2851)
                     /gene="yidG"
                     /locus_tag="ACHK3A_001177"
     CDS             complement(2489..2851)
                     /gene="yidG"
                     /locus_tag="ACHK3A_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO8739291.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(2841..3188)
                     /gene="yidH"
                     /locus_tag="ACHK3A_001178"
     CDS             complement(2841..3188)
                     /gene="yidH"
                     /locus_tag="ACHK3A_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO8739292.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(3248..4741)
                     /gene="yidJ"
                     /locus_tag="ACHK3A_001179"
     CDS             complement(3248..4741)
                     /gene="yidJ"
                     /locus_tag="ACHK3A_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8739293.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKS
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGDNILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(4738..6453)
                     /gene="yidK"
                     /locus_tag="ACHK3A_001180"
     CDS             complement(4738..6453)
                     /gene="yidK"
                     /locus_tag="ACHK3A_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO8739294.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     MGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            6620..7486
                     /gene="yidL"
                     /locus_tag="ACHK3A_001181"
     CDS             6620..7486
                     /gene="yidL"
                     /locus_tag="ACHK3A_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8739295.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(7576..9237)
                     /gene="yidE"
                     /locus_tag="ACHK3A_001182"
     CDS             complement(7576..9237)
                     /gene="yidE"
                     /locus_tag="ACHK3A_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO8739296.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(9433..9861)
                     /gene="ibpB"
                     /locus_tag="ACHK3A_001183"
     CDS             complement(9433..9861)
                     /gene="ibpB"
                     /locus_tag="ACHK3A_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO8739297.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(9973..10386)
                     /gene="ibpA"
                     /locus_tag="ACHK3A_001184"
     CDS             complement(9973..10386)
                     /gene="ibpA"
                     /locus_tag="ACHK3A_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO8739298.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(10380..10452)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            10691..11023
                     /gene="yidQ"
                     /locus_tag="ACHK3A_001185"
     CDS             10691..11023
                     /gene="yidQ"
                     /locus_tag="ACHK3A_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO8739299.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(11025..12239)
                     /gene="yidR"
                     /locus_tag="ACHK3A_001186"
     CDS             complement(11025..12239)
                     /gene="yidR"
                     /locus_tag="ACHK3A_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO8739300.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHQLDSTLDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSNEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            12340..13404
                     /gene="cbrA"
                     /locus_tag="ACHK3A_001187"
     CDS             12340..13404
                     /gene="cbrA"
                     /locus_tag="ACHK3A_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO8739301.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTMLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(13401..14738)
                     /gene="dgoT"
                     /locus_tag="ACHK3A_001188"
     CDS             complement(13401..14738)
                     /gene="dgoT"
                     /locus_tag="ACHK3A_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8739302.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(14813..15961)
                     /gene="dgoD"
                     /locus_tag="ACHK3A_001189"
     CDS             complement(14813..15961)
                     /gene="dgoD"
                     /locus_tag="ACHK3A_001189"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO8739303.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(15958..16575)
                     /gene="dgoA"
                     /locus_tag="ACHK3A_001190"
     CDS             complement(15958..16575)
                     /gene="dgoA"
                     /locus_tag="ACHK3A_001190"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO8739304.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEKSIPAIVDAYGDKALIGAGTVLKPEQVDALARMSCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(16559..17437)
                     /gene="dgoK"
                     /locus_tag="ACHK3A_001191"
     CDS             complement(16559..17437)
                     /gene="dgoK"
                     /locus_tag="ACHK3A_001191"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO8739305.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGFNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(17434..18123)
                     /gene="dgoR"
                     /locus_tag="ACHK3A_001192"
     CDS             complement(17434..18123)
                     /gene="dgoR"
                     /locus_tag="ACHK3A_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO8739306.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            18400..19056
                     /locus_tag="ACHK3A_001193"
     CDS             18400..19056
                     /locus_tag="ACHK3A_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739307.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(19100..19912)
                     /gene="yidA"
                     /locus_tag="ACHK3A_001194"
     CDS             complement(19100..19912)
                     /gene="yidA"
                     /locus_tag="ACHK3A_001194"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO8739308.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTTLSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(20027..20425)
                     /gene="yidB"
                     /locus_tag="ACHK3A_001195"
     CDS             complement(20027..20425)
                     /gene="yidB"
                     /locus_tag="ACHK3A_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO8739309.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            20543..20644
                     /locus_tag="ACHK3A_001196"
                     /pseudo
     CDS             20543..20644
                     /locus_tag="ACHK3A_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20665..23079)
                     /gene="gyrB"
                     /locus_tag="ACHK3A_001197"
     CDS             complement(20665..23079)
                     /gene="gyrB"
                     /locus_tag="ACHK3A_001197"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO8739310.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(23108..24181)
                     /gene="recF"
                     /locus_tag="ACHK3A_001198"
     CDS             complement(23108..24181)
                     /gene="recF"
                     /locus_tag="ACHK3A_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO8739311.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(24181..25281)
                     /gene="dnaN"
                     /locus_tag="ACHK3A_001199"
     CDS             complement(24181..25281)
                     /gene="dnaN"
                     /locus_tag="ACHK3A_001199"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO8739312.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(25286..26689)
                     /gene="dnaA"
                     /locus_tag="ACHK3A_001200"
     CDS             complement(25286..26689)
                     /gene="dnaA"
                     /locus_tag="ACHK3A_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO8739313.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            26986..27066
                     /locus_tag="ACHK3A_001201"
     CDS             26986..27066
                     /locus_tag="ACHK3A_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739314.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            27296..27436
                     /gene="rpmH"
                     /locus_tag="ACHK3A_001202"
     CDS             27296..27436
                     /gene="rpmH"
                     /locus_tag="ACHK3A_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO8739315.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            27453..27812
                     /gene="rnpA"
                     /locus_tag="ACHK3A_001203"
     CDS             27453..27812
                     /gene="rnpA"
                     /locus_tag="ACHK3A_001203"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO8739316.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            27776..28033
                     /gene="yidD"
                     /locus_tag="ACHK3A_001204"
     CDS             27776..28033
                     /gene="yidD"
                     /locus_tag="ACHK3A_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO8739317.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            28036..29682
                     /gene="yidC"
                     /locus_tag="ACHK3A_001205"
     CDS             28036..29682
                     /gene="yidC"
                     /locus_tag="ACHK3A_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO8739318.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            29788..31152
                     /gene="mnmE"
                     /locus_tag="ACHK3A_001206"
     CDS             29788..31152
                     /gene="mnmE"
                     /locus_tag="ACHK3A_001206"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO8739319.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            31395..31469
                     /gene="tnaC"
                     /locus_tag="ACHK3A_001207"
     CDS             31395..31469
                     /gene="tnaC"
                     /locus_tag="ACHK3A_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO8739320.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            31690..33105
                     /gene="tnaA"
                     /locus_tag="ACHK3A_001208"
     CDS             31690..33105
                     /gene="tnaA"
                     /locus_tag="ACHK3A_001208"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO8739321.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            33197..34444
                     /gene="tnaB"
                     /locus_tag="ACHK3A_001209"
     CDS             33197..34444
                     /gene="tnaB"
                     /locus_tag="ACHK3A_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO8739322.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            34576..35751
                     /gene="mdtL"
                     /locus_tag="ACHK3A_001210"
     CDS             34576..35751
                     /gene="mdtL"
                     /locus_tag="ACHK3A_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO8739323.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            35726..36685
                     /gene="yidZ"
                     /locus_tag="ACHK3A_001211"
     CDS             35726..36685
                     /gene="yidZ"
                     /locus_tag="ACHK3A_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO8739324.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            36842..37591
                     /gene="yieE"
                     /locus_tag="ACHK3A_001212"
     CDS             36842..37591
                     /gene="yieE"
                     /locus_tag="ACHK3A_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO8739325.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            37613..38179
                     /gene="yieF"
                     /locus_tag="ACHK3A_001213"
                     /gene_synonym="chrR"
     CDS             37613..38179
                     /gene="yieF"
                     /locus_tag="ACHK3A_001213"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO8739326.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(38233..39570)
                     /gene="adeP"
                     /locus_tag="ACHK3A_001214"
     CDS             complement(38233..39570)
                     /gene="adeP"
                     /locus_tag="ACHK3A_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO8739327.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            39737..40402
                     /gene="yieH"
                     /locus_tag="ACHK3A_001215"
     CDS             39737..40402
                     /gene="yieH"
                     /locus_tag="ACHK3A_001215"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO8739328.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            40469..40942
                     /gene="cbrB"
                     /locus_tag="ACHK3A_001216"
     CDS             40469..40942
                     /gene="cbrB"
                     /locus_tag="ACHK3A_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFO8739329.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITHLPGLDISCPETDSLS"
     gene            complement(41024..41746)
                     /gene="yieK"
                     /locus_tag="ACHK3A_001217"
     CDS             complement(41024..41746)
                     /gene="yieK"
                     /locus_tag="ACHK3A_001217"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO8739330.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLHNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLIVIADKAAAAELALG"
     gene            complement(41761..42930)
                     /gene="yieL"
                     /locus_tag="ACHK3A_001218"
     CDS             complement(41761..42930)
                     /gene="yieL"
                     /locus_tag="ACHK3A_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO8739331.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPALKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(42957..44573)
                     /gene="bglH"
                     /locus_tag="ACHK3A_001219"
     CDS             complement(42957..44573)
                     /gene="bglH"
                     /locus_tag="ACHK3A_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO8739332.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(44659..46053)
                     /gene="bglB"
                     /locus_tag="ACHK3A_001220"
     CDS             complement(44659..46053)
                     /gene="bglB"
                     /locus_tag="ACHK3A_001220"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO8739333.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(46072..47946)
                     /gene="bglF"
                     /locus_tag="ACHK3A_001221"
     CDS             complement(46072..47946)
                     /gene="bglF"
                     /locus_tag="ACHK3A_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO8739334.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQSAQVAQQEKTPEVITPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAI
                     MPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(48080..48916)
                     /gene="bglG"
                     /locus_tag="ACHK3A_001222"
     CDS             complement(48080..48916)
                     /gene="bglG"
                     /locus_tag="ACHK3A_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO8739335.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIVKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRIIE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT        12039 a        12838 c        12145 g        12071 t
ORIGIN      
        1 aaaaaccccg ccggagcgag gtttcgtcag tcgcctgcgg ctggtaaccg caaagcacac
       61 tgtattatgt caacactgaa agtatacgtg ttccgcgcag aacgcgcaat ttcggcacga
      121 attttgacgt atttagtgca tagttgagta tcgatcacag tttgcgtttt gtccaaaatt
      181 actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt tatgcggggg
      241 ccgcatcgtt acccggcgca ctaagtcctg gcagaaacgg gtggtgccgt cagcgcctta
      301 accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt gagataccgc
      361 gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc
      421 acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac tcattgttgc
      481 agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat tcaagtcgca
      541 ctaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg gtgtcggatg
      601 cgatgctgac gcatcttatc cgccctacca tctctcccgg caacatttat tgccgctttt
      661 gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg ctaacattca
      721 tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta ttctaattat
      781 ttttataaaa gcatccgtga taatgaaaag gcatagaaac gtcaatttgt tattgatgtt
      841 ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag ctattgccga
      901 tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct
      961 gctgacttac ggtgtctcac agctgtttta tggcccgatt tccgaccgcg tgggtcgccg
     1021 accggtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg cggtcacgac
     1081 ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca ccggcgttgg
     1141 cggcgtaatg gcgcgtactt tgccgcgcga tttatatgaa cggacacagt tgcgccatgc
     1201 taacagcctg ttaaacatgg ggattcttgt cagtccgttg ctcgcaccgc taatcggcgg
     1261 tctgctggat acgatgtgga actggcgtgc ctgttatctc tttttgttgg ttctttgtgc
     1321 cggtgtgact ttcagtatgg cccgctggat gccggaaacg cgtccggtcg acgcaccgcg
     1381 cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta actgttattt
     1441 gctgatgctg attggcggtc tggccggaat tgccgccttt gaagcctgct ccggcgtgct
     1501 gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta ttctgccgat
     1561 tccggcggca ttttttggcg catggtttgc cggacgtccc aataaacgct tctcaacgtt
     1621 gatgtggcag tcggttatct gttgcctgct ggctggcttg ctgatgtgga tccccgactg
     1681 gtttggcgtg atgaatgtct ggacgctgct cattcccgcc gcgctgttct ttttcggtgc
     1741 cgggatgctg tttccactgg ctaccagcgg cgcgatggag ccgttcccct tcctggcggg
     1801 caccgctggc gcgctggtcg gcggtctgca aaacattggt tccggcgtgc tggcgtcgct
     1861 ctctgcgatg ttgccgcaaa ccggtcaggg cagcctggga ttgttgatga ccttaatggg
     1921 attgttgatc gtgctgtgct ggctaccgct ggcgacgcgg atgtcgcatc aggggcagcc
     1981 tgtttaagct cacgtcaccg cagcatcgtc atcagctcca tgggagaacg atgctgcttt
     2041 atcagatcac gcatcacccg catatgcggc gcggagtaag aataaaacgc ctgatagccc
     2101 gcacaaagca cgttgtctgg tttatcttcc cgagcgtgcg ggcacagccg atgccagtcg
     2161 cacgcctggc agtcacggcg cggttgcgac ttttcgctgc cacaccattt ttgtaaggtg
     2221 gtgtcaaaaa gttcaatcga aatacgactg atatctgccc gcacccaaca ctcgaacagg
     2281 gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag atcgccccgg
     2341 tcatcaaatt caagatgggg aataagctgt actgagcgga tgcccagctg tttgaaattg
     2401 tgataaaaat cggcaggttg cagagcatcc ttatactcta caaccagttt atgcctgtcc
     2461 tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat acgataagtt
     2521 gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg gagatcaaaa
     2581 atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc attaaattgc
     2641 gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca atcgaaatcc
     2701 agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc aacgcgccgt
     2761 agcccagcat ggtacgaaac cacgccagtg atgtgcgttc cggctgcagc cctgggtcgg
     2821 caatgcgtct ggctttgcgg ctatccggca tacaacacca gtcccatgac gatcactgcc
     2881 acgaccatca atattaaact gatgattaat aagctgttgg tatagggcag gtcctctttc
     2941 aggcgcatcg ctttttcatt gcgtaaccag cgcaagtagc catacatcgc taacccgccg
     3001 gaaaacagac acaacaacaa cgccagcagt tcgcggatga cgggcgtggc gaaatctggc
     3061 gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcagtacg gatccacgcc
     3121 agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc gaggcgggaa
     3181 attttcattg ttgctccttg taaactgttc ctcgccgaaa aattaggcca ggaacagcaa
     3241 taatgcatca gaacttctgt tttttctctt ccaccttcac cccttgtgtc ggtaaaccgg
     3301 tgtcatagtc gcgtaccact ggagaataac catcctgtgg gcgtggacga aacgccccca
     3361 tccagcgcgg ttgtgcatct ttgcgccacg gacgcaggtt ccattgataa ctgcggaacg
     3421 gatcgcgaat tttgtccata taatccaata aggcgtcatg cattttgctg cgaacgtcgg
     3481 caaaatggat atcatcgatc aggttatgca tttcatttgg gtcattacgg cgatcgtaaa
     3541 gttcatcact ggtgaagagg ttgagcacca gtttaaagtc atccgttacc cagcaacgca
     3601 ccggaataaa accgccaaag ctgtcatgct caatctcgta gcgattaaat tccaccaaca
     3661 cgccacgtgg ttcttttacg gcaaggatat tatcccccgg cagaatctct ggtttttcaa
     3721 tatctgccag cgccatcatt gtcggcagta agtcaatatg actgactggc gtatcgacct
     3781 gtcgccgctc cccttgcggc gaacggatga tcagcggaat gcgggtgatg tcgtcataca
     3841 tcgccgcccc tttactgatc agcttatgtg cgcccatcat ttcaccatga tcggaggtat
     3901 aaatgaccca cgtattttca cgttgctctg gcgttaaggc attgatgaca cgtccgattt
     3961 ggtcatcaac aaagtcattg caggcaaaat agagcggatg gtgataaagc ccgtcatcac
     4021 cgactggcga tggcatcgcc tgcgcccata agcggtgatg ttccggtttg ttcgccaggt
     4081 catcctgtga tttctcgcca agttcgtagt aaaaatcagc gtatttctct aaatactcca
     4141 ccggacaggt gaacgggtga tgcggctcat catacgaaac caccatcagg aaaggttcgt
     4201 cggcgcgcgc gggctgttgc agaaaatcta ccgcccgatt gctgatgcgg tgcgcccagg
     4261 tgaaggtttc gtcgatatgg ttcgcctgta aatcctcaac gctgtttagg ccattgcgcc
     4321 acaggctgat ctctttttcc gtcagttcgc taaggtagtt cgccccatcg aaccagtaat
     4381 cagcgtccca ctccggcgga cactcgccag tgccgaaata gtcatgaccg tcgagatgcc
     4441 atttgccaat gtaacaggtg tgatagcccg catccttaaa gtagcgcccc atggtggaaa
     4501 tgtttttacc tggcgcgacg ttgttggtcc acgggccgga ctggttagcg tagataccgg
     4561 taaacagccc ggcgcgcgct ggtgtacaaa ccggtgaaca ggtataggcg gaattaaagc
     4621 gaatactttc cgccgccaga ctatcaatat tttgcgtatt cagcggctta ccgctatagc
     4681 aaccgaccat attggtggcc tgggtatcgg tcatgatgaa cagaaaattg gggcgtttca
     4741 taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat gccgccagct
     4801 atcaaaaatc aggtagacaa tcactacggc ggcaatgaaa taactgatca tcgctaacca
     4861 gcgcgtaccg tagccgccga attcagccag cccggcatag acgccaatca tcgcgaacag
     4921 gatgcccatt gacgcgatct tgacgttttt ccacggtttc atgtccaccg caaacgcatc
     4981 tttgaaggtg aacggcgtgg cgcgcggttt gataaaaccg atcaccagca tcacgaccac
     5041 gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact tcaccagata
     5101 gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg ccagcgccgg
     5161 gatgcgcggg aagaaaaagc ccataatgat gatggtaacc agcggcacgt tgtaaatgcc
     5221 gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc acggcgctac
     5281 cagcaccgaa acgatggcga taaagaaacc aaatttgcgc ccgacggtga ccagctgctg
     5341 cggctcggca ttctggttaa tgatgcgccg gtaaataccc atactgaata acgtactggc
     5401 gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg cgccgaagaa
     5461 acccaccagt ggcactggca gaacgttatt gaccagcgtc gggtaggcca tgtcggcttt
     5521 cggtagatcc tgatacagat gaaacgcaat caaccctggc agcaccagta ccagcgggtc
     5581 gagcattttc agcaccgccg ttaacagcgc ccccttttgc ccttccgcca ggctttttga
     5641 cgccagcgtg cgttgcacga tgccctgatt tgtacaccag taaaaggtgt tcaccagaat
     5701 caaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccgccga ctgagtttaa
     5761 tttctcggcg tgaacggtgg tgagttgctc aatgccctgc ataaagctgc ccttgcccat
     5821 cgcgatcagg ccaaataccg gcaccatcaa cccgccaata accagcccaa taccgttaat
     5881 ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa ttcccgccag
     5941 accaagcaaa ataaccagta accagatagc cgcaccatcg gaaagttgta gcgactcacc
     6001 cacatgaaac aggctgttga gcgccaacgc gccggaatag agaacaatcg gcagaaagca
     6061 gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt tatcataacg
     6121 ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg gtaaaaagat
     6181 tagcgcgagg aagatcagcg tcactgcaga ggtgacttcc cagcccatca ccgacatgcc
     6241 acttttgtag gcctgcccag aaaggccgac cagttgttcc gtggaaaggt tggttagcat
     6301 taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat agccttgttg
     6361 cgatccggta tctgttttac ggaccttcca ccaggtgatg accgctacca gcagcgtaaa
     6421 accgacaaaa ctcaagattt gtaacgaatt catctcatag cccttattta tgtttatgtc
     6481 ccggacgcat gaatataggg agaggtaaaa tgcgtcgggt caggcagtca gattcttaaa
     6541 atacaaacgt cgtatccctg aacggattgt tttctgttaa gttcaggttg tgagcataat
     6601 tcgccggagg gatattaaaa tgaatggaaa attgcaaagt tcggatgtaa aaaacgaaac
     6661 tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa tttctctgat
     6721 atcgctctgg catacctacg ccgacgagca ttatcgggtg atctggccgc gggacaagaa
     6781 gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat gtgggaaaat
     6841 tcttttaaag aatgacgaac aaataactct gcatggcaac tgtattatat ttttaaagcc
     6901 aatggatatt cactcttatc actgtgaagg tttggtctgg gaacagtatt ggatggaatt
     6961 tacccccacc agtatgatgg atattcccgt cggtcagcaa agcgttattt ataatggcga
     7021 agtttataat caggaactca ccgaagttgc tgagttaatt acttcaccag aagcaataaa
     7081 aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgca
     7141 cgcagtcggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca
     7201 tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga
     7261 ggcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct
     7321 cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caagggaact cggttggcga
     7381 agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaagcataa
     7441 atttggttat gcgccgtcag ctgtgctgaa gaatacggac aggtaataac cagaatggcg
     7501 agtgtgtaaa tgactcgatg atgttcagaa ataccaggtg gagtaccatt tacggcgact
     7561 ccacccggaa aagagttaac cgatactcca gaagagcacc gccagtaatt ggggggtgat
     7621 aatgcgcagg aacatcacca acggatagac agtggcgtaa gagagcgccg ccgcaccgct
     7681 cgttggatga agattattag caaatgccag cgccggagga tcggtcatgg agccagccag
     7741 catcccgcac atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt
     7801 aatcagcgga acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatc
     7861 caccagtgta tggataaaat cgccgccgga tttcaggcca accaccgaga gaaagagcac
     7921 aatgcctaac tcgcgtagcg ccaggttagc gcttggtggc ataaaccagt acagcttgcc
     7981 gatactgccg atacgcccga ggatcaacgc cataatcagc ggaccgcccg ccagcccgag
     8041 tttcaacgcc gccgggaacc caggcacaaa gacgggaata gaacctaaca atacgccaag
     8101 cccgatacca ataaacactg gcagcatctg aacctgttgc agtttttgct gcgcattccc
     8161 cagcacattg gcaacggcat caattgcgga cggacgcccc accaggttga ggatatcgcc
     8221 gaactgcagg ctgatatcgc tacttgcaac taattcaaca ccagcacggt tcaggcgcga
     8281 gataacaacg tcatagcgtt ctttaaagtg caggtcgcga atacgtttac cgagcacgtt
     8341 ttcattggtg accaccacac gctcgacgcg caagtcagtg cctttcgtag acagcgaggt
     8401 atcgacctcc tgaccaatca ccagttgcgc attatgtaaa tccgcaggct gccccaccag
     8461 atgcagcaaa tcgcccagtt gaataacggt ttctggcgaa ggcactttta gggtttcttc
     8521 tcgtttcaat cgtgagcaaa taactttatc gccattgaga atcggcacat ctttaatggc
     8581 taaattatgc aaattaggat tctcaactcg gatgttaatc gtcctgatta gcgcaccgcc
     8641 gttggttcgc gtggactcgt gctgctgggc ctcagtctca acattgacgc ggaaaataac
     8701 ccgcagcatc cacatagtga acaaaatgcc gcaaatccca aatggatacg ccatcgcata
     8761 gctcatcccc atttgatcga ccatcgccat cggtgtcccc aggtcgcgca agatctgctg
     8821 ccctgccccc agtgctggcg tattggttac cgcaccggag aaaatcccca gtactaccgg
     8881 cagtggaata tcaaacagtt tatgcaggat ggcggtaacc agccccccga tgatgacgat
     8941 cagaacagca aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg
     9001 ccctacctga atcccgatgg tataaacgaa caggatcagg ccaaattcct gaataacatg
     9061 cagcatatcg ctacttaatg tcatccccgc ctgagaaaca aaatggccga cgatgatccc
     9121 accgaacagc acgccgccaa tgcccaatcc aacaccgcga aacttgacgt tgccgataaa
     9181 caacccgacg accgccacca aagccagaat actgaccgtt aatgctatat cactcatcgt
     9241 ccatttctct tgagataaca ttttaattgc tacggattat ggctgaatcg gtgaagcaga
     9301 tttgccttat agcgcacatt atcacgggaa agaacgtgcc gaaatatctt aaacaggcga
     9361 aaattaacgc attgttgaag aagaaagaca aaaaaagccc caccagaatg gcggggcaaa
     9421 gagaatagct agttagctat ttaacgcggg acgttcgctg atagcgatac gctgcgctgc
     9481 gatgggttca ggctcattac gaattaaatc aatatgcagt aaaccgttta cgaaggttgc
     9541 accagagact tccatatttt cagccagcgt aaagctcagg ctaaatggct gattcataag
     9601 cccttgatgc agccattttt tctcttcttt tggctgctcc ggcgtgcctt ttacgctcag
     9661 gcgcgtacct tccagttgaa tctctaaatc ttcctgacga aaacctgcca gcgcaagggt
     9721 aatgcggtag tggttatcgt cgcttttctc aatgttgtac ggcgggaagc tctggctttc
     9781 accggcgttt tgcagtgcgt tggccagttt gtcaaaaccg atccattgac gcatcagtgg
     9841 ggataaatcg aagttacgca tagtcatttc tccttctaag aagcgagtaa gtacctgcaa
     9901 atccgaagat tcgcatatgc tccctgacgg cgagcatgga gatgtcaggc cgcgcaaggc
     9961 ggccttaggg aattagttga tttcgatacg gcgcggtttt ttcgcttccg gaatcacgcg
    10021 ttcgagatcg atatacagca aaccatttac caggttagca ccacgaacat gaatgttctc
    10081 agctaactgg aatttgcgtt caaagttgcg ttcagcgatg ccctggtaca gataggtgcg
    10141 ctctttttgt tcgtcggcgt gagcaccttt caccaccagc agattatcct gggcggtaat
    10201 ttccagttcg ctctcagcaa aaccagccac agcgatagca atgcggtaat ggttttcgtc
    10261 taccagttca acgttatacg gagggtagcc gccattactc tggctctggt tgttttctaa
    10321 gtggttaaac aaacggtcaa atccaatagc agaacggtaa agcggggata aatcaaagtt
    10381 acgcataatc aatagctcct gaaatcagcg agaatctaag accttccaca atggacaggt
    10441 caggtagcca gaacacccat caggcgcgtc ctcatcggct acgatgtaaa aatgggtctg
    10501 gaaatgaact tttcaagcct tatcagcgga ctttttttgc agtttatggt ctattgcata
    10561 gactgagggg gcagcacgaa acgttaaagt gcgatagccg ccacagtgcg acgtaatgat
    10621 gatgttattt tcagcaaggg atcgctataa ctcatcaggc caacacccac gatcaacaga
    10681 tgaatttatt atgataagaa atgtgttgtt agcgttcatg atatgcagcg gaatgacatt
    10741 acttggagga tgctccagcg tgatgtccca taccgggggg aaagaaggaa cgtatcccgg
    10801 cacccgcgcc agcgctacca tgattggcga cgatgaaacc aactggggca ctaagtcgct
    10861 ggcgattctc gatatgccgt ttaccgctgt gatggacacg cttttactgc cgtgggacgt
    10921 gttccgcaaa gacagctcgg tgcgctcacg cgttgaaaaa agcgaagcta acgcgcaagc
    10981 caccaatgcg gttattccac ccgccagaat gcctgataac tgatttagcg atcagtttcg
    11041 gtgatccaca gctggccgcc ttccatccac gccagccact gaccatccgg cgagaagacc
    11101 acggcgtccg cagaaggtgg attggcgtgg ttttcggtta aatactcaac ctcgccgctt
    11161 tgcgcatggg cgcaagcaat tcgattatcc agcacaaagc ccaaccattc tcctgatgga
    11221 tgccagttaa atgcagactg aatatccgtt ttgttatggg ttaactggcg cggctcgccg
    11281 ccctgtggcg agataagcca cagttgcaca atgccgttat catcacgcat taaaaacgcg
    11341 atttgcgtac cctgcggatt acagcgcacc cagtggcgcg ggacgttgac taaccccgga
    11401 taagcccgat ggtgggtaaa ggttaaacgt cgctgcacga cgccacgcgg tggcgcgggc
    11461 agggttgttt ccgttccact taacggcgca tcaccagctg ctttccagcc agcttcatct
    11521 tgcggtaact caacgataaa tagctccggc actttctcgc cctttggcga aagtgtgtcg
    11581 ccaataaacg ccagcgcgtg atttcctacc catccttctt cataagcacg attgatttca
    11641 ttactgccag gctgcggcgt gggcgttgtt ttactcacca gcacgcacca gtggctaccg
    11701 ctgtattcac gcggatgctg cttttgtacg ttgaccgggc caaacggcgc agcaacacct
    11761 acgtttcgca aatccagcgt cgaatcgagt tgatgcatta catggtcgtt ataggtaaag
    11821 ctcacccttt ctccgttcgg gctaaagaca tgcacatggc tgccgccgcg cagcgctcct
    11881 ggggtgtacg gcgcggtaat atccattgca tcgagattgc tcaccttgcc gccttcagca
    11941 atcactccgc gccgatgatg gaagtcgtaa taccatgttt catcaggatt ttccgggccg
    12001 tggatgaaaa catatttctc tgacttcgga tgaacggtca ccacgccgac gtacgcgccc
    12061 tgtgacgcgc gatagataac ctcgacctcg ccggtatgga tattcacacg ctcaatggtc
    12121 tcgccggtaa acgacgcccc tgaaggacgc acgtcaaata ccagccactg gctatcgggc
    12181 gtccaggtat tggtattggt gagcaggtga ttacggggag caaaggttat ctgtttcatg
    12241 gcgcggtcct gatacaaaac gggtcccgcc atcatacttc acaaggactt cactttcaga
    12301 caatagtctt ttgccatcgt gatgaatgtg agtgaaaaga tggaacattt cgacgtggcg
    12361 attattggcc tcggcccggc agggtcggcg ttggcgcgaa agttagcagg caaaatgcag
    12421 gtgatcgcgc tggataaaaa gcaccagcat ggtactgaag gtttcagcaa accttgtggc
    12481 ggtctgctgg caccggacgc gcagcgatct tttattcgcg atggactgac gcttcctgtc
    12541 gatgtcatcg ccaacccgca gattttcagc gtcaaaaccg tcgacgtcgc cgcatcgctc
    12601 acgcgtaact accagcgaag ctatatcaat attaatcgcc atgctttcga cttgtggatg
    12661 aaatcgctga tccccgccag cgttgaggtt taccacgaca gcctgtgtcg aaaaatctgg
    12721 cgtgaggatg ataaatggca tgtcattttt cgcgcagacg gctgggagca gcatattacc
    12781 gcccgctatc tggtcggtgc cgatggtgcc aactcgatgg tgcggcgaca tctctacccg
    12841 gatcatcaaa ttcgtaaata tgtcgctatc cagcagtggt tcgcagagaa acatccagtg
    12901 ccgttctact cctgcatctt tgataatgcg ataactgact gttattcatg gagtatcagc
    12961 aaagacggtt attttatctt tggcggtgcc tatcccatga aagacggtca gacgcgtttc
    13021 acgatgctga aagagaaaat gagcgccttt cagttccagt ttggtaaggc ggtgaaaagc
    13081 gaaaaatgca cggtgctgtt tccctcacgc tggcaggatt ttgtctgcgg taaggacaat
    13141 gcctttctga ttggtgaagc ggcgggattt atcagcgcca gctcgctgga ggggattagc
    13201 tatgcgctgg atagcgcaga gattctgcgt tcggtgttac tgaagcagcc ggagaagagc
    13261 aacgccgcct actggcgcgc cacccgcaaa ctgcgtttaa aactcttcgg caagatagta
    13321 aaaagccgat gcctgaccgc accggcttta agaaagtgga ttatgcgcag tggtgtggcg
    13381 catattccac agttgaaaga ttagccaacg cgcttcacat cgcccaccag caggatgtaa
    13441 gagagcgcgc caatcaacgc gacggcggag atataaacca gtgcaggtgc gaaaccgtaa
    13501 ccctgcgcca ggtaccccac caccagcgga acggtgatgc cgcccagacc accggcgaag
    13561 ttaaacacgc cgccggttaa accaatcagg cgcatcggtg ccagagaaga gaccagcgac
    13621 caggtaatcg aagcaaaacc gttgccgaag aatgccagcg ccatcaggca cataatcatc
    13681 atcggatcgt tggtgtagtt agcgcccata atgcaggtgg agatcagcaa gccgcagata
    13741 atcggcgttt tacgcgcaaa gcccagtgaa aagcccttac gtaccagcag atccgcgacc
    13801 cagccagaga gcaggacgcc gacaaacgcc gcgaggaatg gcaccgtggt cataaagcct
    13861 gctttcagcg ccgtgattcc tttttcctgg gttaaatagt tcgggaacca agttaagaaa
    13921 aaccacagtg tagaagccac cgcaaattgc ccaagataga cgccgatcag tttacgatgg
    13981 aacaccagtt tccagtcttt ggctgttaac ggctgacgcg cctctttttt caccggcgca
    14041 tcaccatcca ccagaccgcc gccatcacga atgtaatcca gttcagcttt gctgatgcct
    14101 ttagtcaggc gcggcggctg ataaacctta aaccaaatca gcgaccagat aatgccgata
    14161 ccaccggtga caatgaacac ccagtgccag ctcaacattt cctgaatcca gatcagcagc
    14221 ggagtcaaaa acgccagacc gacaaactga ccagacgtat aaaaaccaac ggcggaagcg
    14281 cgttcatgtt ccgggaacca gctggtcacc atccggttat tggtcgggaa agcgggcgct
    14341 tcgaaaatac cggttattgc gcgcagacca attaatgaca tcaatcccgt ggcaaagccc
    14401 tggaataaag tcgccactga ccagccaaat atcgcaataa aataagtcac gcgtgaacct
    14461 acgcgatcta aaaaccaacc gccggggatc tgacatagtg tataaagcca ggcgaaggcc
    14521 gaaaatacat agcccatttc cgctttggta ataccgaact cttcctgaat atgggcggaa
    14581 gccacggcca ggttagcgcg gtcaacataa caaatgacca ccgtaataaa gatcatcacc
    14641 agcgtcagat aacgccgacg ccccggcttt gctgcattaa cgggaatatc catagcgagc
    14701 tttctccaga ttttgggcat agcgaagccg ctcaccatgc cctgtaattt acagagggtt
    14761 atatttttgt attgctgttt tagtgcccga tgagaggctt acgtggcagg aattaccact
    14821 ctgctacgct gttatcttca tgacgccaga gcggattacg ccagtccggg gcatttttac
    14881 tgaactcaat aactttagct tcgtcgattt ccacacctaa gcccggtttc gttaatggtt
    14941 taaagaaacc gccgaccatg ctgaagtctt ctttgttttt cacaaagtcg agtaactccg
    15001 cgcctttgtt gtaatgaatg cccatacttt gttcctgaag tacggcgtta taggaaacaa
    15061 agtcgatatg caggcaagcc gccagtgcaa tcggtccgag cggacagtgc ggcgcaaggg
    15121 tcacgtcata ggcttctgcc attccggcaa ttttgtagca ttcggtaata ccgcccgcgt
    15181 gggagagatc tggttgcaga atcgaaatac cacccgcctc cagcacacgt ttaaaatcga
    15241 aacgtgagaa catgcgttca cccgccgcca gtggaatatg cgtttgtgcc gccagtttcg
    15301 ggtagtattc ggcttgttct gccagcaccg gctcttcaat aaacagcggg cgatacggct
    15361 ccagttcttt aatcagcact ttcgccatcg gcgcgctgac gcgaccgtgg aaatcaagac
    15421 caaactcaat ctgattgcca aaagcttcac gaatttgtgc cacggtgttg actgccgcat
    15481 ctaccgcgcg ggagttatca attagcccca gttcttcaca accgttcagt ttgaaggtat
    15541 cgaagccgat ttcgcgcagg gttttaatgc cgtcgataac atccgccgga cgatcgccgc
    15601 cgacccaact gtaggcttta attttgtcgc gaaccaggcc gcccatcagt tgccagaccg
    15661 gcgcattcag cactttgcct ttgatatccc ataacgcctg gtcgatcccg gcgatggcgc
    15721 tcatcaggat tggaccgcca cgataaaatc cggcgcgata catcacttgc cataagtcat
    15781 tgatgcgcga tggatcctga ccaatcaaat agtcacccag ctcgtgaact gccgcttcca
    15841 ccgtgcgggc gcggccttca atcactggct cgccccagcc gaccacgcct tcatcggttt
    15901 caattttcag gaacatccag cggggaggta aacgatacgt ggtaattttg gtgattttca
    15961 ttgcactgcc tctcgatacg ccttaacaaa tgctgctgcc tgctgcgcgg tgcgttctac
    16021 ggactgcccg gcgcgataga gatcgctgcc taagcccgcc cctgcacaac ctgcgtctat
    16081 ccactgcgcc aggttttctg gcgtcacgcc gccaacggca aagactgcga tgtccggtgg
    16141 taataccgct tttaacgctt tgatgtattg cggaccaaaa gccgatgacg gaaatatttt
    16201 cagcgcctga gcgcccgcgt cgagcgcggt aaaggcttcg gtcgccgtcg cgcagccggg
    16261 gcaaacggtc atgccgtagc ccaccgcacg gcggatcact tcactatgga tattgggcgt
    16321 aacgatgagc tggcagctca tcctggcgag tgcatcgacc tgttcaggtt tcagtaccgt
    16381 acctgcgcca atcaacgcct tatcgccata cgcatcaacg atggcgggaa tgcttttctc
    16441 ccattgaggg gaattcagcg ggatttcaac cgcgtcgaac ccggcgtcaa tcaccgcgcc
    16501 aacatgcgcc agcgcctcgt cgggcgtaat gccgcgcaaa atggcgatca gcgggagttt
    16561 agtttgccac tgcatgagcg atgctcctta taccagcctg aaatgccgtg tcgcccgcca
    16621 ccgccgtcac gtcgcaaccc atcgcctgaa aggcttgctg gtagcgcgcg gtcagcgatg
    16681 ttccggcgac aagggtgatg gcgtgttgat gggtcacata gtcgcgcata ctggcaacct
    16741 ctgcgccaat caacaaacca gagagaaatt cgctgacctg ttcgcgggga agtgttccca
    16801 gcacatgcga ggcgcgaact tcaaaaagct gcggcaatat ggcgggagca ttaaagccac
    16861 gctcaagacc agccgcgaag gcatcggcag agttttcctg cggcggcaaa cctgcgccaa
    16921 tcaatgagtg atttaacagt aaatgatgta attcaccggt catcacggtg cgaaaatcgt
    16981 tgatttgctg gctatcggcc tgcacccatt tgcaatgggt accgggcata acataaagag
    17041 aggaaggagc gagagcacgc gcgccgatca attgtgtttc ttcgccgcgc atcacattgt
    17101 ggttatcgtc atgagagacg cataatccgg gaataatcca gatattgtcg ccaaccgacg
    17161 ttaattgatc gccaatagac gaaaaacggg caggaacaga taaatacggt gcaactttcc
    17221 agccgacatt gctgccgacc attcctgcca tcaccactgg cgtattctct tcacgccagt
    17281 cggtcgtgac ttctgctaac accgcagccg gagattttcc gttcaggcgc gtgacgcctg
    17341 cttctgattg cctgctgtcc aggcagtggt cgccctgata aagccaggcg cgcagattgg
    17401 tcgatcccca gtcaattgcg atgtagcgag ctgtcatgtg atttccttta accttcgtgt
    17461 cgagctggcg atcatggtaa gcgccgcctg ctctgccgca tcgccgtcct gatgccgtat
    17521 tgcatcgaac agcgccttat gttcctggag cgtttgcggc atgttggcct catcgcccat
    17581 ccaggttcgt tcaaaaaccg cccgctgcag cgaactgatc gcaatgctaa gttgctgtaa
    17641 caccgggtta tgcaccgact gcagcaccgc ctcgtggtag cgaatatccg cttcgttaaa
    17701 cgcttcgcgg tcctgattgt tggcaatcat ctcgttcagc gccgattcaa tctgcgccag
    17761 atcgctggag gtcgcgcgct ctgctgccca acgggcaata gccggctcta ccaggtttcg
    17821 cacttcgctc attgcactga taagccgtgg gtcatagtcg ttttccagca cccattgcag
    17881 tacgtcagtg tcgaggtaat tccactggtt acgcggagcc acaaacgccc cgcgataacg
    17941 tttcatttca atcagccgct tcgccatcag cgaacgaaac acctcacgga tgatgttgcg
    18001 cgaggttgca aactcttcac agagctccgc ctcagccgga agtggagagc ctggtacgta
    18061 tttgccgtga acgatctgtt tacccagcgt aataacgatg cgatcggttt tattgagagt
    18121 catggagagt ccttgtgctc tgtatgttct tctctacttt accccgatcg atgcataacg
    18181 cggcaacttt gtagtaccag cgtgatgacg ttcgcgtttg ctgtgcgcct aatgtagtac
    18241 aaacttatat tgttgtacta caatttagat cacaaaaaga acaatacata aaaaatgaca
    18301 tgcgtcgggc agaaatctga aaagggatgt caggcgctaa acaggaggga aagaagagta
    18361 tgctttcaac ggcttagcta ctcgtttaaa ggattaatca tgaagttgaa ttttaaggga
    18421 ttttttaagg ctgccggttt gtgttctttg gcatttgcgt tgaccggatg tatcacctgg
    18481 ggattggttt ccaatactgc aagccgtcca catgccgagc aatggcggtc agacacgatt
    18541 aagggtttat cgctggctga agacagtaac ggtacgaaag gctatgtttt tgttggtgaa
    18601 tcgttggact atctgttgac caccggcggc gatgaggtgg ttaaaatgct gaacgatccg
    18661 gcaatccatg gtgaaagaat caccgtttct gataacgcga aatttatttt aagcagcagt
    18721 aataagaatt tctctggtgc tattacgctc tattacgact ggaacaacga agaagataag
    18781 gcactggcaa cacaatatgg ttttatttgt gatacccggc gttgtacatg gatgctggac
    18841 ggtttgaaag gctcaatcca ccagaaaaat aaaaaagcgg attactcaaa cgtgatggtt
    18901 ttccatcaac cctttacggt gggattttat gagtacaaag caacagatgg cgtcccgcgc
    18961 ggcctcgtta atgcgctgct gccagtcact cttacgctgg atatagtaac gtcaccactc
    19021 cagttcctga ttttatgcac gaccagaaat tgttaatcgc tgcgggcaaa cgcattcatg
    19081 ccgtttgccc attcactaat taattcagca catacttctc aatagcaaat gccacgccat
    19141 cttcaaggtt ggatttggtg acaaagttcg ccacttcttt caccgaagga atagcgttat
    19201 ccatcgccac accgacgcct gcatattcaa tcattgcgat gtcgttttcc tgatcgccaa
    19261 tcgccatgat ttcttccggt ttaataccta acacgtcggc cagtgatttc acacccgtac
    19321 ctttgttaac gcgtttatcg aggatttcga ggaagtacgg cgcacttttc agcacggtgt
    19381 atttttcttt cacttcctgc ggaatacgcg cgatggcctg gtcgaggatg gcaggttcat
    19441 caatcatcat cactttcagg aactgggtat tggggtccat tttctccgct tcgcagaaca
    19501 ccagcggaat ggtggcaacg aaagattcat gcaccgtgta gtagctgata tcacgattgg
    19561 cggtgtacag cgtggtgcgg tccagggcgt ggaaatgaga accgacttcg cgagagagtt
    19621 tttccaggaa acggtagtcg tcatagctga gagtggtttg cgccacggtg ctaccatcag
    19681 cggccttctg taccagcgcg ccgttataag taatgcagta gtcgcccggc tgttccatat
    19741 gcagctcttt caggtaattg tgcacacctg cataagggcg acccgtcgtt agcacgacat
    19801 tcacgccacg ggcgcgagct gcggcaatcg catttttaac ggcgggtgaa atggtgtgat
    19861 cgggcagcag aagggtgcca tccatatcga tagcaatgag tttgatagcc atgagttccc
    19921 caggtagatt ggttcctgac ccatgctaac gcgattccgc tcaaaaatca gtacaacacc
    19981 cgagggaaaa gggggatgca acgcgcgttg cgtgctcccc ttttgcttag cggaagagtt
    20041 tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt ggtgacactt
    20101 caccttgcgg tgagagtgca tcaataatct tcggcaattg ttctgccagt aaactggaag
    20161 ctgtactggt atccacgccc agtttttgcc cgagatcgga caccgcattt gtgccgagcg
    20221 ccgattccag ttgctcgccg ctaactggtt ggttgccctg ttgattactc agccaggttg
    20281 agagaatggc acctaaaccg ccactttgca gtttttccag cagcacctga atgccgccct
    20341 gctcctcaac ccaacttaaa atcgcctgat atttccccgc atcgcctttc agaaaggcgc
    20401 cgacaacgtc atcaaaaagg cccatgataa tcacctgtaa agcgttacgt gttgactcaa
    20461 aaagtatagt tctgtgggat gataattgcg gattgcagga ataaaaaggg cggagatgat
    20521 ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg aacgccttat ccggcctaca
    20581 aaaacatgca aattcaatat attgcaggag tctcgtaggc ctgataagcg tagcgcatca
    20641 ggcacgctcg cattgttaac gccattaaat atcaatattc gccgctttca gagcgttctc
    20701 ttcaataaac gcacggcgcg gttcaacggc gtcgcccata agcgtagtga acaactggtc
    20761 ggcagcaatc gcatctttaa cggtaacgcg cagcatacga cggctttccg ggtccatggt
    20821 ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct ggatggagag
    20881 gccgcgacgg gactctttca ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg
    20941 acgacgttcg ccacgttcga taaatgcatc ttcttccagc aagccacgca gtttctcacc
    21001 cagcgtgcag atacgacgat attcgccgcc agtgataaat tcgtgatcca gcggatagtc
    21061 agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt gttcggcatt
    21121 ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca gttcgctgac
    21181 cagcgcgttc acccagcggg taacggtctg ctcatcagag aggtcggctt ccgtcagcgt
    21241 cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca tgcggttgat
    21301 cattttctgc gtcgcgttgt actcagacac cagtttctct aacgcttcgc cagccaatgc
    21361 cggtgcactg gcgttggtgt gcagcgttgc gccatccagc gcgatggaga tctggtactg
    21421 atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca ctttgtacag
    21481 cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa
    21541 gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat
    21601 gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac cacaaccaag
    21661 cgcggtgata agcgtcgcca cttcctggga agagagcatc ttatcgaagc gcgctttctc
    21721 gacgttgagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg
    21781 cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcagaga gcgccggatc
    21841 gcgttcctgg cagtctgcca gtttgcccgg caggcccgct aagtcgagcg cacctttacg
    21901 gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat cgataatttt
    21961 gccaaccacg attttcgcgt cggttgggtt ttccagcagg tattctgcca gcagttcgtt
    22021 catctgctgc tcaaccgccg atttcacctc agaagaaacc agtttgtctt tggtctggga
    22081 ggagaatttc gggtccggca ctttcacgga aacgaccgca atcaggcctt cacgcgcatc
    22141 gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca tgtaggcgtt
    22201 cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg
    22261 aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact gcaacgccac
    22321 ttcgacgcca ataccgtctt tctcggtgga gaagtagaag atattcgggt ggatcggcgt
    22381 tttgttcttg ttcagatatt caacgaacgc cttgatgccg ccttcatagt ggaagtggtc
    22441 ttctttgccg tcgcgcttgt cgcgcagacg aatggaaacg ccggagttga ggaacgacaa
    22501 ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg tgaaggtttc
    22561 gaggcttggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg taaccgccag
    22621 cggggcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct cgcgctggat
    22681 aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc cgtgcagacc
    22741 gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat
    22801 cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc
    22861 atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt tacagtgacc
    22921 cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt gcagaccggt
    22981 gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat ccagcccttt
    23041 caggactttg atactggagg agtcataaga attcgacatc aacgtttctc gctcatttat
    23101 acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg
    23161 acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt
    23221 ttaagcggct ggcaagcagc ccgcgacgct catcatcaag ctcagaggca aaatcatcta
    23281 tcaggtagag acaccgccgc ccgctttcac gggtgaggaa ctctccttgc gccagacgta
    23341 aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc ggcgcaccgt
    23401 cggcgcgaat gcgtaagtcc gctttatgcg ggccgtgcgc ggtgtaggtt agctggcgat
    23461 cgcgttcaaa attacgttcc agcacctcag cgtactctgt ctctttctcc cagccgcgct
    23521 ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatcg gccatatcag
    23581 ccgcgatacc ggcgctatac tccgcgcgcc aggtgcttat ttgctccgcc agcggaatca
    23641 gttctttatc ccacgggcgt agctgttcgt aacgtgtcac ctggcgcagc gccgcattgc
    23701 gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccgggt tcgttgtgaa
    23761 agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt aaagtaaacc
    23821 cttctggcgt tatcaactgc attggcatca ggtgcgccag ttccgcgacc ttatgcccgt
    23881 ctgtaccgtc gatgcggact ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg
    23941 tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa cgcctcctgc tcgtggcgaa
    24001 tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta tagatggctt
    24061 ccagcacgct ggttttgcca ctaccgttgg cacctaccag aaagttaaag ccgggagata
    24121 aagcgagatc cgcggtttcg atattgcgga aatcgcggat caacaagcgg gtgagggaca
    24181 ttacagtctc attggcatga caacataggc agccgactgt gatgcggcat cttcaatctg
    24241 cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca gtgcgttcag
    24301 aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc tataggtaac
    24361 gtcgaggatc tcttccgctt cttcctgttc tgggttgttg gcggtgattt tcagctggtt
    24421 ttcgctgaca tacagacgca cgccgcggaa tttctcgtta gagagaattg ccgcacgggc
    24481 aaacgcctgc ttgagcagat cacagccagc ttccagatgc ttgtccgggt tcttcggcag
    24541 aacgcgacgg taatccggga agcgaccatc caccagtttc gaggtgaaga taaagtcgcc
    24601 aacgtgggcg cgaatgttgt tgctgccaat ctgcacgcgc agcggattgt cgccgccgtc
    24661 gagcatacgc atcagttcaa tcacgccttt acgcggcacg atcaccgaat ggcttggcaa
    24721 agattgacca attggcattg aacagactgc cagacggtgg ccgtcggtcg ccacggtgcg
    24781 cagttcttca ccttcggttt caaacagcat accgtttaag taatagcgaa cgtcctgatg
    24841 cgccatagaa aactgggtcg cttcaatcag acgcttcatc gtcgcctgcg gcagggtaaa
    24901 ttcgacttcg ctctgccagt catcgaggtt cgggaaatct gccgctggca gggtagacag
    24961 cgaaaaacgg ctacgcccgg agcgcaccag catccgctca ccttccagct gcacggcaat
    25021 ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt
    25081 cgcccctggt tcgtgcggct gaaccagcgc aacgcgtgcc accatttcca tctcgagatc
    25141 agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac cgagaatcgg
    25201 tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta ataaatgctc
    25261 acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa ttagaaaaat
    25321 cttctttgat atcgtggctc tcttcacgca actgctcgat cttccggcag gcatgaagca
    25381 ccgtcgtgtg gtcacgacca ccaaaggcat cgccaatctc cggaagactg tggttagtca
    25441 gctctttcgc cagcgccatc gccatttggc gcggacgggc caccgagcgg gatcgacgct
    25501 tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc tgaatattgt
    25561 cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt
    25621 cgatggtgat cgcccgtccg gtaaagttgg cattggcaat gacgcggttc agcgcgcctt
    25681 ccaactcacg tacgttagat cgtagacgct tggcgataaa aaacgccact tcgcccggca
    25741 aacgaatgtc gttttcgtcg gcctttttca tcaggatcgc cacgcgggtt tccagctctg
    25801 gcggttcgat cgccacagtc agtccccagc cgaagcggga tttcaaacga tcctcaacgc
    25861 cgttgatctc tttcggatag cgatcggagg tgagaatgat ctgttgatta ccttccagca
    25921 gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc tttattagca aaaaactgaa
    25981 tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct tcgatcgcgt
    26041 tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata taaaccactt
    26101 tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg tgagttttac
    26161 ccaaacccgt gccgccataa aggaacaacg ggttataagc accgccaggg ttatctgcca
    26221 cctggcgagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag ttatcaaacg
    26281 tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggagccggg acgttatccc
    26341 aacccgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg
    26401 ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt
    26461 caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca ttgatattat
    26521 taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac agggccagcg
    26581 tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct gtggctggta
    26641 actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg gcggactcca
    26701 ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga cgtacgtcga
    26761 aaagaccctg catgagggtg acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga
    26821 tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga tcttctgttt
    26881 ctcacagatt ttcccgattt atccacagga ctttccagaa ctcgctaagt gtaaacgatc
    26941 ctgccgcggg ccgggcacga tttacgccgc atattggaaa atttaatgac catagacaaa
    27001 aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac ccatcagccc
    27061 gtataatcct ccacccggcg cgccatgctg gttttcactg gtgtgaggcc gtacattttc
    27121 cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact ccggagtgta
    27181 caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc gtttcatttt
    27241 tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa tcgccatgaa
    27301 acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc gtgctcgtat
    27361 ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct
    27421 gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt cccagggagt
    27481 tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa cgggctggca
    27541 cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt atcggtctta
    27601 cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa cgtctgacgc
    27661 gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg gtggtggcga
    27721 aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa aaattatggc
    27781 gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct atcaacgcct
    27841 gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa gctacggaat
    27901 tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga aacgcgtatt
    27961 aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac catttgatac
    28021 cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg
    28081 tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca ggcccaacag
    28141 accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt accggccagt
    28201 ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat caacacccgt
    28261 ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa ctctacccag
    28321 ccgttccagc tgctggaaac ttcaccgcag tttatttatc aggcacagag cggtctgacc
    28381 ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa cgttgaaaag
    28441 gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac gtataccgac
    28501 gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac
    28561 gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctcctc gtttggtcag
    28621 ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa cttcgcactg
    28681 cacaccttcc gtggcgcggc gtactccacg cctgacgaga agtacgagaa atacaagttc
    28741 gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg ggtggcgatg
    28801 ctgcaacagt atttcgcgac ggcatggatc ccgcataacg acggtaccaa caacttctat
    28861 accgctaatc tgggtaacgg catcgccgct atcggctata aatctcagcc ggtactggtt
    28921 cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga aatccaggac
    28981 aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg gttgtggttc
    29041 atttctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg taactggggc
    29101 ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg
    29161 cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc aatgcgtgag
    29221 cgtctgggcg atgacaaaca gcgtatcagc caggaaatga tggcgctgta caaagctgag
    29281 aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat cttcctggcg
    29341 ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc
    29401 cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg cgtaacgatg
    29461 ttcttcattc agaagatgtc gccgaccact gtgaccgacc cgatgcagca gaagatcatg
    29521 acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg tctggtgctg
    29581 tactatatcg tcagcaacct ggtaaccatt atccagcagc agctgattta ccgtggtctg
    29641 gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga gttttcgcta
    29701 aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa gcaccgcaca
    29761 tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt agcccaggcc
    29821 acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa
    29881 gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta tcttccgttt
    29941 aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc tggcccgaac
    30001 tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt gatcctcgac
    30061 ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc gggtgagttt
    30121 tccgaacgcg cgtttcttaa cgataaactc gacttagccc aggccgaggc gattgcggat
    30181 cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct gcaaggcgca
    30241 ttctccgcac gggttaatca tctggtagaa gccctcaccc acttgcgcat ttacgttgaa
    30301 gcggcaattg atttccccga tgaagagatc gatttcctct ccgacggcaa aattgaagcc
    30361 cagcttaatg acgtgattgc cgatctcgat gcagtgcgtg ctgaagcacg tcagggtagt
    30421 ttgttgcgcg aagggatgaa agtggtgatt gccggacgtc ctaacgccgg taaatcgagc
    30481 ctgttaaacg cgctggctgg ccgtgaagca gcaatcgtaa ccgatatcgc cggaactacg
    30541 cgtgacgtgt tgcgtgagca tatccacatt gacggaatgc cgctgcatat catcgacacc
    30601 gccgggctac gtgaagccag cgatgaagtg gaacgtattg gtatcgagcg cgcgtggcaa
    30661 gaaattgaac aagccgaccg cgtgctgttt atggtcgatg gcaccacgac agacgccgtt
    30721 gatccagcag agatctggcc ggagtttatc gcccgtctgc cagcgaaact gccgatcacc
    30781 gtggtgcgca ataaagccga tatcaccgga gaaacgttgg ggatgagcga agtgaacggt
    30841 cacgcgttaa ttcgtctctc tgcaaggact ggtgaaggtg tggacgtgct gcgtaaccat
    30901 ctcaaacaga gcatgggctt tgacaccaac atggaaggcg gcttcctggc gcgtcgtcgt
    30961 cacctacagg cactggaaca ggcggcagag cacctgcaac agggtaaagc gcaactgtta
    31021 ggagcctggg caggcgagct gctggcggaa gaactgcgtc tggcgcagca gaacttaagc
    31081 gagatcaccg gggaatttac ttctgacgac ctgctggggc gaattttctc cagcttctgt
    31141 attggtaagt aaccgcgctt gcgaagccgc attctgactg tcagatgcgg cttcgcttca
    31201 ttgttagcac tcctgttact cctcaaccct ttttttaaac attaaaattc ttacgtaatt
    31261 tataatcttt aaaaaaagca tttaatattg cttcccgaac gattgtgatt cgattcacat
    31321 ttaaacaatt tcagaataga caaatttctt gagtattata ctgtagcctc gtgtctttcg
    31381 aggataagtg cattatgaat atcttacata tatgtgtgac ctcaaaatgg tttaatattg
    31441 acaacaagat tgtcgatcac cgcccttgat ttgcccttct gtagccatca ccagagccaa
    31501 accgattaga ttcaatgtga tctatttgtt tgctatatct taattttgcc ttttacaaag
    31561 gccatctttc gtttatttac ttgttttagt aaatgatggt gcttgcatat atatctggcg
    31621 aattaatgga tattgcaaat gtaatattca cagggatcac tgtaattaaa ataaatgaag
    31681 gattatgtaa tggaaaactt taaacatctc cctgaaccgt tccgcattcg tgttattgag
    31741 ccagtaaaac gtaccactcg cgcttatcgt gaagaggcaa ttattaaatc cggtatgaac
    31801 ccgttcctgc tggatagcga agatgtgttt atcgatttac tgaccgacag cggcaccggg
    31861 gcggtgacgc agagtatgca ggccgcgatg atgcgcggcg acgaagccta cagcggcagc
    31921 cgcagctact atgcgttagc cgagtcagta aaaaatatct ttggttatca atatactatt
    31981 ccgactcacc agggccgtgg tgcagaacaa atctatattc cggtactgat taaaaaacgc
    32041 gagcaggaaa aaggcctgga tcgcagcaaa atggtggcgt tctctaacta tttctttgat
    32101 accacgcagg gccatagcca gattaacggc tgtaccgtgc gtaacgtcta tatcaaagaa
    32161 gccttcgata ctggcgtgcg ttacgacttt aaaggtaact ttgaccttga gggattagaa
    32221 cgcggcattg aagaagttgg cccgaataac gtgccatata tcgttgcgac catcaccagt
    32281 aactctgcag gtggtcagcc ggtttcactg gcaaacttaa aagcgatgta cagcatcgcg
    32341 aagaaatacg atattccggt ggtcatggac tccgcacgct ttgctgaaaa tgcctatttc
    32401 atcaagcagc gtgaagcaga atacaaggac tggaccatcg agcagatcac ccgcgaaacc
    32461 tacaaatatg ccgatatgct ggcgatgtcc gccaagaaag atgcgatggt gccgatgggc
    32521 ggcctgctgt gcatgaaaga cgacagcttc tttgatgtgt acaccgaatg cagaaccctt
    32581 tgcgtggtgc aggaaggctt cccgacatat ggcggcctgg aaggcggcgc tatggagcgt
    32641 ctggcggtag gtctgtatga cggcatgaat ctggactggc tggcttatcg tatcgcgcag
    32701 gtgcagtatc tggtcgatgg tctggaagag attggcgttg tctgtcagca ggcgggcggt
    32761 cacgcggcat tcgttgatgc aggtaaactg ttgccgcata tcccggcaga tcagttcccg
    32821 gcacaggcgc tggcctgcga gctgtataaa gtcgccggta tccgtgcggt agaaattggc
    32881 tctttcctgt taggccgcga tccgaaaacc ggtaaacaac tgccatgccc ggctgaactg
    32941 ctgcgtttaa ccattccgcg cgcaacatat actcaaacac atatggactt cattattgaa
    33001 gcctttaaac atgtgaaaga gaacgcggcg aatattaaag ggttaacctt tacctacgaa
    33061 ccgaaagtat tgcgtcactt caccgcaaaa ctgaaagaag tttaattaaa acactcagag
    33121 tggctataag gatgttagcc actctattac cctacatcct caataacaaa aatagccttc
    33181 ctctaaaggt ggcatcatga ctgatcaatc agaaaaaaaa cactctgcat tttggggtgt
    33241 tatggttata gccggtacag taattggcgg tgggatgttt gctttacctg ttgaccttgc
    33301 cggagcctgg tttttttggg gcgcttttat tcttattatc gcctggtttt caatgttaca
    33361 ttcagggcta ttattattag aagcaaactt aaattatccc gtcggatcca gttttaacac
    33421 catcaccaaa gatttaatcg gtaacacctg gaacattatc agcggtatta ccgttgcctt
    33481 cgttctctac atcctcactt atgcctatat ctctgctaat ggtgcgatca ttagtgaaac
    33541 gatatcaatg aatttgggtt atcatgctaa tccacgtatt gtcgggatct gcactgccat
    33601 tttcgttgcc agcgtattgt ggataagttc gttagccgcc agtcgtatta cctcattgtt
    33661 ccttgggctg aagattatct cctttgtaat tgtttttggt tctttcttct tccaggtcga
    33721 ttactccatt ctgcgcgatg ccaccagcac cacggcggga acatcttatt tcccgtatat
    33781 ctttatggct ttgccggtgt gtctggcgtc atttggtttc cacggcaata ttcccagcct
    33841 gattatttgc tatggcaaac gcaaagataa gctaatcaaa agcgtggtct tcggttcgct
    33901 gctggcgctg gtgatttatc tcttctggct ctattgcacg atggggaata tcccgcgaga
    33961 aagctttaag gcgataatct cctcaggcgg caacgttgat tcgctggtga aatcgttcct
    34021 cggcaccaaa cagcatggca ttatcgagtt ttgcctgctg gtgttctcca acttagctgt
    34081 tgccagttcg ttctttggtg tcacgctggg attgttcgat tatctggcgg atctgtttaa
    34141 gattgataac tcccacgccg ggcgtttcaa aaccgtgctg ttaaccttcc tgccacctgc
    34201 gttgttgtat ctgatcttcc cgaacggctt catttacggg atcggcggtg ccggactgtg
    34261 tgccaccatc tgggcggtca ttatccccgc ggtgctggca atcaaagctc gcaagaagtt
    34321 tcccaatcag atgttcacgg tctggggcgg caatcttatt ccggcgattg tcattctctt
    34381 tggtataacc gtaattttat gctggttcgg taacgtcttt aacgtgttac ctaaatttgg
    34441 ctaaatcctt caagaagcca gccattcgct ggcttcttgc ctctcaggaa atcacttatg
    34501 tccaaatggc aactcgcctg atcctccttc accacgtatg ctttgcgtca ccttactatc
    34561 aggacgcttt agcccatgtc ccgctttttg atttgtagtt ttgccctggt tttactttat
    34621 cccgcaggga ttgatatgta cctcgttggt ttaccgcgca tcgccgccga tctcaatgcc
    34681 agcgaagcgc agttgcatat tgcgttctcc gtttatctgg cggggatggc ggctgcgatg
    34741 ttatttgccg gtaaagtggc cgatcgttca gggagaaagc ctgtcgccat acccggcgcg
    34801 gcgctattta ttatcgcctc ggtgttctgt tcactggctg aaaccagcgc gttatttctt
    34861 gcagggcgat ttctacaggg actgggcgcg ggctgttgtt acgtagtggc gttcgccatt
    34921 ttgcgcgaca cgctggatga tcgacgtcgg gctaaagtgc tgtcgttact caacggcatt
    34981 acctgcatca ttccggtgtt agcgccggtg ctcggacacc tgattatgct taaattccct
    35041 tggcagagtc tgttctggac gatggcaacg atgggcatcg cggtactgat gttgtctttg
    35101 tttattttaa aagaaacgcg ccctgcggcc cccacaactt cggacaaacc acgagaaaat
    35161 agcgagtcac tgcttaatcg gtttttcctc agccgtgttg ttatcaccac cctcagcgtt
    35221 tcggtgatcc tcaccttcgt caacacgtcg ccagtattgc tgatggaaat catgggtttt
    35281 gagcgcggag aatacgccac cattatggcg ttgaccgctg gcgtcagcat gaccgtttca
    35341 ttctccacgc cgtttgcgct gggaattttt aagccacgta cgttgatgat cacctcacag
    35401 gtgttattcc tggcggcggg gatcaccctt gccgtttcac cttcccatgc ggtttcgctg
    35461 tttggtatca cgctgatttg cgccggtttc tcggtaggtt ttggtgtggc gatgagtcag
    35521 gcgttagggc cgttttcatt acgcgcgggc gtagccagct cgaccttagg tattgcgcaa
    35581 gtttgcggtt cgtcactgtg gatttggctg gcggcggtgg ttggtatcgg cgcatggaat
    35641 atgctgatcg ggattctgat tgcctgtagc atagtgagcc tgttgctgat tatgttcgtc
    35701 gcgcctggac gcccggttgc cgctcatgaa gaaatccatc accacgcttg atctcaatct
    35761 gctgctctgt ctgcaactgc tgatgcagga gcgcagcgtg accaaagcgg cgaagcggat
    35821 gaacgtgacc ccttcggcgg tgagtaagtc gctggcaaag ttaagagcgt ggtttgacga
    35881 cccgctcttt gtgaactcac cgctggggct gtcgcccaca ccgctaatgg tcagcatgga
    35941 gcaaaatctg gcggagtgga tgcaaatgag caacctgctg ctggataaac cgcaccacca
    36001 gacaccgcgc ggcctgaagt ttgagctggc agcagaatca ccgctaatga tgatcatgct
    36061 taatgcgctg tcgaaacgga tctaccaacg ttacccgcag gcgaccatca aattacgtaa
    36121 ctgggattac gattccttag atgccattac tcgtggtgaa gtggatattg gtttttccgg
    36181 tcgcgaaagt catccccgtt cgcgggagct gttaagctcg ctaccgttag ccattgatta
    36241 tgaagtgctg tttagtgatg tgccctgcgt ctggttacgc aaagatcatc cggcactgca
    36301 tgaagcatgg aatctggaca cctttttacg ttatccgcat atcagcattt gctgggaaca
    36361 gagcgatacc tgggcgctgg acaatgtgtt acaggagctg gggcgcgaac gcacgattgc
    36421 tatgagcctg ccggaattcg agcagtcact gtttatggca gcacagcccg acaatctgct
    36481 actggcgacc gcaccgcgct actgtcagta ctacaatcaa ctccaccaac tgccgttggt
    36541 tgctcttcct ctcccgtttg acgaaagcca gcaaaaaaag ctggaagtcc cttttaccct
    36601 gctgtggcat aaacggaaca gccataatcc gaagatcgtc tggttacggg aaaccattaa
    36661 aaatctttac gcgtcgatgg cataaccgaa tcgtatgaaa cgcgacccaa tttcacaata
    36721 aaatgtaaaa aagttgtaat aagcttgtct gaatcgaact tttagccgct ttagtctgtc
    36781 catcattcca gtaaatgatt actcttgtat tcataatgga ccattaagca tggagcgaaa
    36841 aatggcgact cactttgccc gagggatttt aacggaagga catctgattt ctgttcgtct
    36901 cccctcccag tgtcatcaag aagcccgaaa cattccccct catcgtcaaa gccgttttct
    36961 ggcgtccaga ggtttactcg cagaactgat gttcatgctg tatggcattg gcgaattgcc
    37021 ggaaatcgtc accctgccga aaggaaaacc ggttttcagc gataaaaatt tgccttcgtt
    37081 ttctatttcc tatgccggga atatggtcgg cgtggcgtta acaaccgaag gtgaatgtgg
    37141 cctcgatatg gaactacagc gtgcgacgcg cgggtttcat agcccacacg cgcccgataa
    37201 ccacaccttt tccagcaatg aatcgctatg gatcagtaaa caaaacgatc ctaacgaagc
    37261 gcgggcgcag ctcatcacgc tgcgccgaag cgtgctaaaa ctaaccggtg atgttttgaa
    37321 tgacgatccg cgcgatctgc aactactgcc cattgccgga cgcctgaaat gtgctcatgt
    37381 aaatcatgta gaagcgttat gcgacgcgga ggacgtgctg gtgtggtccg tggcggtcac
    37441 gcccacgatt gaaaagctca gtgtctggga gttagatggc aaacatggct ggaaaagcct
    37501 gcccgatatt cacagccgcg ccaacaatcc taccagccgg atgatgcgtt ttgcccagct
    37561 ctctaccgtg aaggcttttt cgccaaattg atagacaacc acaggagtca tcatgtctga
    37621 aaaattgcag gtggttacgt tactggggag cctgcgcaaa ggctcattta atggcatggt
    37681 tgcacgtacc ctgccgaaaa ttgctccggc gagcatggaa atcaatgcgt taccttccat
    37741 tgccgacatt cccctgtatg acgctgacgt acagcaggaa gacggttttc cagcaacagt
    37801 tgaagctctg gcggaacaga tccgtcaggc tgacggtgtg gtgatcgtca cgccggaata
    37861 taactactcg gtaccgggtg ggctgaaaaa tgccatcgac tggctttccc gcctgccgga
    37921 tcaaccgctg gctggtaaac cggtattgat tcagaccagc tcaatgggcg tgattggcgg
    37981 cgcgcgctgt cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa
    38041 caagccggaa tttatgggcg gcgtgattca gaacaaagtt gatccgcaaa ccggggaagt
    38101 gattgatcag ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat
    38161 tcagcgagtt aagatctaaa taaaaaaccc gccagcaatc atgcatggcg ggtttttaac
    38221 gcgctatcga ttttagtgag cgtcgataaa gacaatcttc aggataaaca gcagcgcaac
    38281 gatgattacg cacgggctaa ggtcacgcag acgtccggta ccaatcttca tcacgcagta
    38341 ggagataaag cccagcgcga taccttcagt aatcgagaag ctgaacggca tcatcacagc
    38401 ggtaataaac gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga
    38461 ggtcatcaac acgccaacgt aaatcagcgc accagctgca gcgtagcctg gcaccatccc
    38521 cgccagcggc gacaggaaga taaccagcag gaacagcaga ccaacaacca ctgccgtcag
    38581 accggtacga ccgccaacgg aaacgccgga agaagattca atatacgccg taacagaaga
    38641 agtaccgata aacgaaccgg tcaccgaaga gatactgtcg acatacagcg cctgcttcat
    38701 gcgcgggaat ttacctttct catcagccag acctgcttta tcggtcacgc caatcagcgt
    38761 accggaggag tcaaacaagt tgaccagcat gaaagagaaa atcacccctg ccagcccaag
    38821 gttaaacgac ccggctaaat ctacatgacc cacgacactc attacgctcg gcggcgcaga
    38881 aacgatgcca ttatagtgga catcacccag catccagccc agcagcgttg tcaccacgat
    38941 ggaaaccagc accgccgcgt gaatattgcg cgaggccaga atggcgatga tgaagaagcc
    39001 gaggatcccc agcagtacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg
    39061 gttagcgaca atcacacctg cattcttcag tcccatcatg ccaataaaca ggccgatacc
    39121 gctggtaatg cccacacgca gactcaccgg aatgttggct atcatccagt agcgaacgcg
    39181 gaaaatcgtc agtaacagca gaccaatcgc gccccagaag attgcgccca tcccgacctg
    39241 ccacggcaag cccatcgcct gcacaaccac aaaagcgaag aacgcattca ggcccatagc
    39301 gggtgccagt gcaactggca ggttagcaaa cagtcccatc ataatactgc cgaatgcagc
    39361 aatcagacag gtagtaacga agacggcgct ggtatccatg ccagcaacgc caagaatttg
    39421 cgggttaaca aaaacgatgt aaaccatcgt caggaaggtg gtaaaaccgg cgatcacttc
    39481 ggtccgtgcc gtcgtgccat gttcgcgcag tttaaacacg cgttccagca tcccctggcc
    39541 agaagcctgg gttgtgtgtt gttgactcat tatctatttc cgaacaagga gggaaaatcc
    39601 gtcgctatcg tataccaaaa tgagacaata tggacggctg aagataactt tttttcattg
    39661 tatttgctta tacggcaacg attgcgtgat gcgaattggc atgacgaaaa cgttaaacta
    39721 acgataaagg aaagacatgt cccagataga agcggtattt ttcgactgcg acggtacgct
    39781 ggtcgatagc gaggtcattt gctctcgcgc ctatgtcacg atgttcaggg aatttggcat
    39841 tcacgttgac ctggaagaga tttttacccg ttttaaaggc gtaaaactct acgaaatcat
    39901 cgacatcatc agtaaagaac agggcgtgac gatggtccag gccgatgccg agcacgttta
    39961 ccgcgcagaa gtagcacgtc tgtttgatac cgaacttgag gacatcgcag gagcgaacga
    40021 attgctggcg agcattaacg tgccgatgtg cgtcgtctct aacggtccgg tcagcaaaat
    40081 gcagcattcg cttggtaagc tcaacatgct gcactacttc ccggaaaaac tgttcagcgg
    40141 ctacgatatc cagcgttgga agcccgaccc ggcgttaatg ttccatgcgg caaaagcgat
    40201 gaatgtgaac gttgaaaact gcattctggt cgatgactcc agcgccggtg cgcaatcagg
    40261 aatcgatgct ggtatggaag tgttttactt ctgcgctgac ccgcacaata agccgatcgt
    40321 tcatccgaaa gtcaccacct ttacccatct ttcgcaatta ccggaactgt ggaaggcgcg
    40381 tggttgggat attacggcat agttattcac actcccttca cttactccac ttaaattggc
    40441 acgcaaaggt aagtaaaggg agtttgatat gtctgtttca cgtcgggtaa ttcatcacgg
    40501 actgtatttt gcggttttag ggccgttaat tggtgttctg tttcttgtcc tctacatatt
    40561 cttcgcaaaa gaaccgctga ttcttttggt aataatacag gtacttccat tgtttctctt
    40621 aatgtcgata actacgggag ctattcctgc aatgttaact ggcgtaatgg tcgcctgtct
    40681 gccggaaaag atcgggtcac agaaacgtta tcgttgtctg gttggcggca taggtggagt
    40741 cgttatcacc gagatctatt gcgctgttat cgtacatatt aaggacatgg cttcctcagc
    40801 gttgtttgaa aacattcttt ctggtgaaaa tctcgttgtc cgcatcatcc cagcattgct
    40861 ggcaggcgta gtgatgagca gaatcattac acatctaccc ggattggata tttcatgtcc
    40921 tgaaacagac tctttaagct aagcggaatg ctttatctat ttgtcattaa atgttggaga
    40981 acaggccgga gcgtaattca cacatccggc cttattttta agcttaaccg agcgctaatt
    41041 ctgcggcagc ggctttatca gcgatgacta tcagcgaagg gtgcaactgc aatacggaag
    41101 caggaacatc ttcggtaacc ggaccttgca gaacattttt cagtgcctgc gctttacccg
    41161 cgccactgac aattatcagc aagtttttcg ccgccatgat gcttttcggc cccatcgtca
    41221 cgtagctgtc aggcactaat gaaaagtcgc cacccaactc gccatgcgcc acaatatcaa
    41281 ccatctctcc ctggatcggg aactccacgg tttgctcatg gaaatgggtc gtatttggca
    41341 gattgccaca aaaatgacca tctacaccta atcccaacac caccaaatcc aggccgcctt
    41401 cacgcgccag tttctgatca tgctcgcggt agttatcaat agtgagcttc tggatattct
    41461 cttctttgat ccctgcaggg gtgaaaaaca gattatgcag attggtaatc gttacgcctt
    41521 ccccttcttt accgcgaaat ggaatttcat cgaaattgta gaaatagcag ttatcgtacc
    41581 agggcttacc tttaaccaga gtgatgagat attcgtacat gcctttgggc gtgctcccgg
    41641 cggtaattgc caggttaacg cgacgcatct ttgacatata accaagcaga tgatgtgccg
    41701 cgacacggct catttcctgg taatcttcgg taatgatcaa tttcatctta atgccttata
    41761 ttatttgaat aatttctgta caaaggctgc ataggctggt cgccagacat ccatttcatg
    41821 gttcagaccg ggatattcct ggtaatcaaa cttaattttt ttctgctcaa gctcagtttt
    41881 cagcccggcg atatccttgc cggttacgac atctttatcg cccacgacca cagtaaaatt
    41941 acgtagttgc tggttaatag ctgccggctc gttcagccgg gcagcgaccc cttcatctgg
    42001 tacggtcgtc gtggtaacac cactgaatgt ggccagccag ccaaagcttt ccagatgatt
    42061 cattcccgat accagtgcct gatacccacc ttgtgaaagc cctgccagtg cgcgaccatc
    42121 ggcatcttta cggacattaa aacgcttgct aatcagcggg ataatatcgt tcatcaattc
    42181 gcgatctgcc gctttagcat ttaacggata aaagacttta cgtctctcct gaggaacgaa
    42241 atcttcggga ataatgccct tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg
    42301 tttaattttc ccttcagcca gcaggttatc catgatttgc gggatacgcc cctgatcaat
    42361 agcggaacgt ccggtatcac caaagccgtg atagaaatag agcactggca aaggctcgcc
    42421 tatgccggtg tatcctggcg gggtccagac atacatctga cgttcggatt gcaatgcgtt
    42481 ggagtggtaa gttatggcga tcaaatcacc atgcacaaca gaacgtgtat ccagataact
    42541 gcccggtacc agaatcatac tggagttaac ctggcgctga ggcttagtca ttgctgtacc
    42601 tgtatcaatg ctgcgtacac catcaacatt gaaaaaatac tcgtacaggt tgcctttcag
    42661 agcaggcgtg cgccacgacc agactcccgc ttcgtctttg gtcatcgggt gaatattgtc
    42721 cggaaccgga acacccacca ctacagagac attttttgcc ccaggggcaa agtagcgaaa
    42781 ggtcacgctg ttatcggtat tgacctgagt cacatactgt ttcaccggaa tagtgggtgc
    42841 cgggctggct ggcatatccg ctgctatggc ccattgagca gaaataccgc tggcaatagc
    42901 cagcgttaaa gcagcaattt ttatattcat cgtaatattc cttttattaa gttccattac
    42961 caccagattt cagcctgggc accgacagca aactggtcgt ctttattgtc ttcaaaggtg
    43021 aagccatcca gttcgttttc cagagctttg atatatgtag cgtagaaacg aatttccggg
    43081 cgcgaggtca acatactggt attgacttta aaggtatgga agagcgtggt tttataacca
    43141 gactcattat atttattgct attcgcatct ttgttttgct gggtgaaata gcccagttca
    43201 acacctgtct ggttatattg gtcccaaata taggccgggc gaacaaccgc acgaatagat
    43261 tcgaaatcag aatgtgcgcc tgtttcgtag ctataaatat cgttaccgaa ggagtaaaca
    43321 atcgcgttag ccacaatgaa atgatcgccg atataagctt cgccctgcga agtcagacga
    43381 actgcagttc cgcctgtgtg atcaccataa taacgaccat taaaggtagt aaatggactt
    43441 gcaccagcat aacgaccaaa gttactggcg atagagttat tcgcaaccag gaaggagaat
    43501 tcgttgaagc cacccttatc aaatttctgc gttaaagatg taccaaacat ccaggtatct
    43561 ttccagtcat aatacccgtt attatcctga ttatcttttt ccgatgcgct ttcatttgct
    43621 gagacataac gaccgctcac cattaaggtg gctttatccc ataacgggat atctttatag
    43681 cgtaaatcaa tggtattggt attaatctgc tgtttgtttt gcaggctgcg atcgtaatca
    43741 tcgacatctt cacgaaccag cgcgatatca attttacccg gaccgacttt ccagttttcc
    43801 agacctacac ctgcggcagc atcggtacgc tgcgttttcc agtcaagcat ctggatttca
    43861 attttcggcg caccgtgttt acccacccag aaatcagcct ctggcgcaaa gggcaggaaa
    43921 cctttggtgg taacgtacat atcagagaac tgcataaagt tctcaccacc ggcgttatcg
    43981 ccaaaccagc cagtagagta ctgctgacca acgttaccat ccatcatcac gatggcatca
    44041 acccgtttgc cgttttcgtt gtagacacgt tgttttaact gcaaatcaaa ccagccggag
    44101 tattcgttac caaagcggcc cagagaacca attgcccatg atttaggtga accatgagag
    44161 gctgttcccc aaccagaacg gtagtagccg ttgtaactaa agccgatttc atctttcaca
    44221 aatttactga aatctttcag agtcatggaa gaataaatcg agcctgtctc actggcattt
    44281 ttctcttcat ttttaaccag tacagcatca ggctttgcgg cacgactggt cgggaatgga
    44341 ttggcggcat acccttgatc attcgtactt acgctacgat tcaccgtcgc tggcgtatac
    44401 tttttcttct cttcatcttt atattttttc agttcgcttt gcgtttctct taacgccttt
    44461 tctaatagct caaggcgttg ttccactgtt aatgattcag ccaatgattg tgcggaaaag
    44521 gctaacggtg ccattaataa gatggcagag gtaataagat ttcgtctaaa cataatatcc
    44581 ctttatggtg caaagaaaga attaacgcat cgcatccaga ctgttctgaa tgcgacgata
    44641 attaaggtgc tttaatggtt atttttttaa tgacagcccc cgagttttga tcacttctgc
    44701 ataccatccg aagcttttct tgcgcgttct tgtgaggctt ccttcaccat tatcatcacg
    44761 atccacataa ataaagccgt agcgcttaga catttgtgaa tgagatgcac tgactaaatc
    44821 aattggcccc caactggtgt accccataat atccacacca tcggcaatcg cttcatttac
    44881 ctgtaccagg tgatcgttta aataggcaat tcgataatcg tcctgtatcg aaccatccac
    44941 ttcaacgctg tcttttgcgc ctaatccgtt ctcgacaata aataacggtt tttgataacg
    45001 atcccaaagc gtatttaaca gaatccgtaa tccaaccgga tcaatttgcc acccccactc
    45061 tgaacttttc agatgcggat ttgggatcat attcagtatg ttgccctgcg catttttatt
    45121 aatgctttcg tcgtgggaaa cacaaccggt catgtaataa ctaaaagaga tgaaatctac
    45181 ggtatgtttt aaatcttctg cgtcactttc agtcatctca atggtgatat tgtggtcgcg
    45241 gaagaaacgc tgcatatagc cgggatactg accacgcgcc tgaacatcac caaagaacat
    45301 ccagcgccgg ttctcttcca tggcctgcaa catatcctgt ggctggcagg tgagggggta
    45361 aaccagccca ccgagaagca tattgccaat tttcgcttcg gggatcaggc tatgacaggc
    45421 tttaactgcc cgcgcactgg caaccagttg atgatggatc gcctgataaa cttccgcctc
    45481 gccactctct tctgccagcc ccactcccgt gaatggcgcg tgtaatgaca tgttgatttc
    45541 attaaatgtc agccataaca caactttatg ttggtagcgg gtgaagaccg tgcgggcgta
    45601 atgctcgaag tgatcgatga ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc
    45661 atatggcatt tcgtaatggg ataacgttac cagcggcttg atccccgcct gcgccatttc
    45721 atcaaacagc cgatcgtaaa acgctaaccc cgcttcattc ggttcggctt cgtcgccctg
    45781 agggaaaatt cgcgcccagg caatggaaat acgcagacag gtgaagccca tctcggcaaa
    45841 taacgcgata tcttccgggt aacggtgata aaaatcgatg gcgacatctt tgatattctc
    45901 tttccccagg atgcgcggtt ccatttttcc cattacgcca tgaggctgta aatctgaggt
    45961 cgtgatccct ttgccatctt cctgccaggc gccttccacc tgattggcag ctgttgcacc
    46021 accccaaaga aatgtttctg gaaatgcttt cataattaac tccttttatc gttagcgaat
    46081 gatggataac agcggctcac cagcgtttat ctgcgccgtg ccgtggggta atacgtccgt
    46141 aaaatcatcg ctattactga ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat
    46201 agcagggata tcaaaagaaa tcagccgatc gcctgtattg accttgtcac ccacgttgac
    46261 gtgagcggaa aagaatttgc cgtccagttt tacggtatcg ataccgacat gaatcaggat
    46321 ctccacacca tcatctgact caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat
    46381 tcgacctgcc accggagaac gcacttcacc aaccgagggc ataatggcaa tacctttacc
    46441 caacaggccg ctggcaaacg tggtatcagc gacgtgaatg agcggcacaa tctctcccgt
    46501 catcggtgaa cagataccgc cctgctcagg tgtaataacc tctggtgttt tctcttgctg
    46561 ggcaacttgc gctgactgac tcttagcggt gatgaaatga aacatcaccg taccgacaaa
    46621 tgcgcaaccg atggcaatga caccgccaat aacgctggcc catacggtga aatcaatccc
    46681 cgttgacggg atggtttgca tgaaggtgaa aatacctggc aaaccaaagg agtaaacttt
    46741 cgtttgcgcg tagccaatga tggtggcccc caatgcccca cttatgcagg cgataacgaa
    46801 ggggtactta cgcggcagat tgacgccata taccgctggt tcggtgatac caaacaaacc
    46861 tgtcaacgcc gctgatcccg ccaccacttt tttctgtgca tcgcgttcgc agaggaagac
    46921 gccgagcgcc gccccgactt gcgccataat ggcgggcatt aacagcggga tcatggtgtc
    46981 gtagcccagc acggtgaagt tattgataat taaaggtatc aaaccccaat gcagaccgaa
    47041 catcacaaag atttgccaga agccgcccat taccgcgcct gcaaatgcag gaaccgcctg
    47101 ataaagccag agataaccgg cagcaatcag ttcacttatc caggttgata gcggccccac
    47161 cagcagaaat gtgatgggcg tgatgaccat cagacatagc aagggtgtga agaaattttt
    47221 gattgccgaa ggtaaccacg cattaagtcg gcgttccaga atgctgcaca accaggcaga
    47281 aaaaataatg ggaataaccg atgacgagta attcaacaat gtgaccggaa tacccaggaa
    47341 atccaggccc agcgcatcca ctttttgccc gttctcgaag gcagtcagaa ttaatggatg
    47401 cactaacgct ccgccaatca ccatggcagt aaatggattg ccgccgaagc gtttccctgc
    47461 ggtgtagccc aggattatcg ggaagaacca aaacaaggca tcactggcgc taaataaaat
    47521 taaataagta ccactttgtt cggtcgtcca ctgaaaagtg agtgccagag ccaacatacc
    47581 tttcaggatc ccggttgccg ccatcaaacc gatcagaggc gtaaaaatac ctgaaataac
    47641 ataaacaaag cggtttaaca gattaccttt atcatcattt tccggtgcct gttgcgcttt
    47701 ttcgccaagg cctgccacgc tgttaaccgc caggaagaca tcggccacat ggttacctat
    47761 gacaacctga aactggccac cgctttccac caccataata ataccggggg tctttttcag
    47821 tacctcagct tgcgctttgc tttcatcctt taacttaaaa cgtaatcgcg ttgcacaatg
    47881 catcagactc acaatgttat ctgcgccccc gactcctgcg actattgttc tggctaactc
    47941 cgtcataact tgccctctac cgctttgcgg caaaactcca aaaaaaaacc tgaaaaaaac
    48001 ggcctgacgt gaatcaagca atttttttca ggttttgccc gcttagtgcg gtaacaatcc
    48061 tttattcagt aataatattt cagtgttctt tgcgcacgcg ctctatattt atggctaaaa
    48121 acataatctc tgcaggtgag attttacgtt gatactgcaa accaataaaa atggcgatcc
    48181 gctccgcaca ttgccatgct tgcgggtaat tttgctttac tgcttgttgt aatgattcat
    48241 cactatcgtt aatcgatgca tgttcaataa tacgccagga taaaaatttc agatgcgtaa
    48301 ccagtcgctg ataactcaag ctttcttcct ggtaattaag gctgaactga aattttatta
    48361 attgcagcat ttctcgcatt aattgcgtga cacctgcaac atcctccata tttccgctca
    48421 tttgggcact gaccagatgc atggcaataa agcccacttc atctttcggt aactgcacgc
    48481 ccaaccgttt aacaataatg gttaacgctt cttcccctag ctggaactct ttcgggtaaa
    48541 gtcgctggat atcccacagc agcgggttgg gcagtagcac gttttgctga aagcgtttaa
    48601 tcgcaaactg gcaatggtca gttagcgaga tataaatact gtcctgcaac tttcccaggc
    48661 gctcctgcgc cagagagata atacgatcac aggttgccat cacctcaaga ggcatatgac
    48721 ttaagagttc gcttaatcgc ccgttcagtt catgactgct caaggcatac tctttttcta
    48781 ttccacttga gttaattctt tcgcctgggc gtttttgaaa gccaattccg cgccccatga
    48841 cgactttttc ccgctgttga tcatcaataa ccaccacaac attattgttg agaattttgg
    48901 tgatttgcat gttcatagca aggacctttt ttataaacaa aaaaacccga cttcaccagt
    48961 attctctggt tatgtcaggt tttgcctgcg aatgcagtaa caatccagtc attaattaat
    49021 ggtttttata acgaacatcc aggttcgaaa attaatttag ttgcgtgcag agaaaatagc
    49081 aatgctctat tta
//
LOCUS       DAZGHF010000019        47595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGHF010000019 DAZGHF010000000
VERSION     DAZGHF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.019"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(310..852)
                     /gene="ycfP"
                     /locus_tag="ACHK3A_001223"
     CDS             complement(310..852)
                     /gene="ycfP"
                     /locus_tag="ACHK3A_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO8739336.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(875..1900)
                     /gene="nagZ"
                     /locus_tag="ACHK3A_001224"
     CDS             complement(875..1900)
                     /gene="nagZ"
                     /locus_tag="ACHK3A_001224"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO8739337.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAREAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHSDPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(1911..2735)
                     /gene="thiK"
                     /locus_tag="ACHK3A_001225"
     CDS             complement(1911..2735)
                     /gene="thiK"
                     /locus_tag="ACHK3A_001225"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO8739338.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2716..3357)
                     /gene="lpoB"
                     /locus_tag="ACHK3A_001226"
     CDS             complement(2716..3357)
                     /gene="lpoB"
                     /locus_tag="ACHK3A_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO8739339.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3371..3748)
                     /gene="ycfL"
                     /locus_tag="ACHK3A_001227"
     CDS             complement(3371..3748)
                     /gene="ycfL"
                     /locus_tag="ACHK3A_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO8739340.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTLPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3751..4110)
                     /gene="hinT"
                     /locus_tag="ACHK3A_001228"
     CDS             complement(3751..4110)
                     /gene="hinT"
                     /locus_tag="ACHK3A_001228"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO8739341.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4444..6633
                     /gene="fhuE"
                     /locus_tag="ACHK3A_001229"
     CDS             4444..6633
                     /gene="fhuE"
                     /locus_tag="ACHK3A_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO8739342.1"
                     /translation="MLSTQFNRDNQYQTTSKPSLLAACIALALLPSAAFAAPATEETV
                     IVEGSATTPVDDENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWAYIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6693..8126)
                     /gene="ptsG"
                     /locus_tag="ACHK3A_001230"
     CDS             complement(6693..8126)
                     /gene="ptsG"
                     /locus_tag="ACHK3A_001230"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO8739343.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8421..9218)
                     /gene="ycfH"
                     /locus_tag="ACHK3A_001231"
     CDS             complement(8421..9218)
                     /gene="ycfH"
                     /locus_tag="ACHK3A_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8739344.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPQDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9229..10233)
                     /gene="holB"
                     /locus_tag="ACHK3A_001232"
     CDS             complement(9229..10233)
                     /gene="holB"
                     /locus_tag="ACHK3A_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO8739345.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFHGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10230..10871)
                     /gene="tmk"
                     /locus_tag="ACHK3A_001233"
     CDS             complement(10230..10871)
                     /gene="tmk"
                     /locus_tag="ACHK3A_001233"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO8739346.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATLPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(10861..11883)
                     /gene="yceG"
                     /locus_tag="ACHK3A_001234"
     CDS             complement(10861..11883)
                     /gene="yceG"
                     /locus_tag="ACHK3A_001234"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO8739347.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKLRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11886..12695)
                     /gene="pabC"
                     /locus_tag="ACHK3A_001235"
     CDS             complement(11886..12695)
                     /gene="pabC"
                     /locus_tag="ACHK3A_001235"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO8739348.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12815..14056)
                     /gene="fabF"
                     /locus_tag="ACHK3A_001236"
     CDS             complement(12815..14056)
                     /gene="fabF"
                     /locus_tag="ACHK3A_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO8739349.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14144..14380)
                     /gene="acpP"
                     /locus_tag="ACHK3A_001237"
     CDS             complement(14144..14380)
                     /gene="acpP"
                     /locus_tag="ACHK3A_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO8739350.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14591..15325)
                     /gene="fabG"
                     /locus_tag="ACHK3A_001238"
     CDS             complement(14591..15325)
                     /gene="fabG"
                     /locus_tag="ACHK3A_001238"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO8739351.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ITDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15338..16267)
                     /gene="fabD"
                     /locus_tag="ACHK3A_001239"
     CDS             complement(15338..16267)
                     /gene="fabD"
                     /locus_tag="ACHK3A_001239"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO8739352.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16283..17236)
                     /gene="fabH"
                     /locus_tag="ACHK3A_001240"
     CDS             complement(16283..17236)
                     /gene="fabH"
                     /locus_tag="ACHK3A_001240"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO8739353.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17304..18374)
                     /gene="plsX"
                     /locus_tag="ACHK3A_001241"
     CDS             complement(17304..18374)
                     /gene="plsX"
                     /locus_tag="ACHK3A_001241"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO8739354.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18455..18628)
                     /gene="rpmF"
                     /locus_tag="ACHK3A_001242"
     CDS             complement(18455..18628)
                     /gene="rpmF"
                     /locus_tag="ACHK3A_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO8739355.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18680..19201)
                     /gene="yceD"
                     /locus_tag="ACHK3A_001243"
     CDS             complement(18680..19201)
                     /gene="yceD"
                     /locus_tag="ACHK3A_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO8739356.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19400..19984
                     /gene="yceF"
                     /locus_tag="ACHK3A_001244"
     CDS             19400..19984
                     /gene="yceF"
                     /locus_tag="ACHK3A_001244"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO8739357.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20097..21056)
                     /gene="rluC"
                     /locus_tag="ACHK3A_001245"
     CDS             complement(20097..21056)
                     /gene="rluC"
                     /locus_tag="ACHK3A_001245"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO8739358.1"
                     /translation="MKTETPSVKIVAINADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21629..24814
                     /gene="rne"
                     /locus_tag="ACHK3A_001246"
     CDS             21629..24814
                     /gene="rne"
                     /locus_tag="ACHK3A_001246"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO8739359.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERAEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQVAEPQPEVAETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            24948..25241
                     /locus_tag="ACHK3A_001247"
     CDS             24948..25241
                     /locus_tag="ACHK3A_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO8739360.1"
                     /translation="MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            25238..25732
                     /locus_tag="ACHK3A_001248"
     CDS             25238..25732
                     /locus_tag="ACHK3A_001248"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8739361.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIDR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(25827..26780)
                     /gene="flgL"
                     /locus_tag="ACHK3A_001249"
     CDS             complement(25827..26780)
                     /gene="flgL"
                     /locus_tag="ACHK3A_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO8739362.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(26792..28435)
                     /gene="flgK"
                     /locus_tag="ACHK3A_001250"
     CDS             complement(26792..28435)
                     /gene="flgK"
                     /locus_tag="ACHK3A_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO8739363.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDASGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDTGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(28501..29442)
                     /gene="flgJ"
                     /locus_tag="ACHK3A_001251"
     CDS             complement(28501..29442)
                     /gene="flgJ"
                     /locus_tag="ACHK3A_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO8739364.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(29442..30539)
                     /gene="flgI"
                     /locus_tag="ACHK3A_001252"
     CDS             complement(29442..30539)
                     /gene="flgI"
                     /locus_tag="ACHK3A_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO8739365.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(30551..31249)
                     /gene="flgH"
                     /locus_tag="ACHK3A_001253"
     CDS             complement(30551..31249)
                     /gene="flgH"
                     /locus_tag="ACHK3A_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO8739366.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(31302..32084)
                     /gene="flgG"
                     /locus_tag="ACHK3A_001254"
     CDS             complement(31302..32084)
                     /gene="flgG"
                     /locus_tag="ACHK3A_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO8739367.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(32255..33010)
                     /gene="flgF"
                     /locus_tag="ACHK3A_001255"
     CDS             complement(32255..33010)
                     /gene="flgF"
                     /locus_tag="ACHK3A_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO8739368.1"
                     /translation="MDHAIYTAMGAGSQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQTTRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(33030..34235)
                     /gene="flgE"
                     /locus_tag="ACHK3A_001256"
     CDS             complement(33030..34235)
                     /gene="flgE"
                     /locus_tag="ACHK3A_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO8739369.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMTLVFNANGVLTSDPTANISTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(34260..34955)
                     /gene="flgD"
                     /locus_tag="ACHK3A_001257"
     CDS             complement(34260..34955)
                     /gene="flgD"
                     /locus_tag="ACHK3A_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO8739370.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGTTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34967..35371)
                     /gene="flgC"
                     /locus_tag="ACHK3A_001258"
     CDS             complement(34967..35371)
                     /gene="flgC"
                     /locus_tag="ACHK3A_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO8739371.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(35375..35791)
                     /gene="flgB"
                     /locus_tag="ACHK3A_001259"
     CDS             complement(35375..35791)
                     /gene="flgB"
                     /locus_tag="ACHK3A_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO8739372.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35947..36606
                     /gene="flgA"
                     /locus_tag="ACHK3A_001260"
     CDS             35947..36606
                     /gene="flgA"
                     /locus_tag="ACHK3A_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO8739373.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            36682..36975
                     /gene="flgM"
                     /locus_tag="ACHK3A_001261"
     CDS             36682..36975
                     /gene="flgM"
                     /locus_tag="ACHK3A_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO8739374.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36980..37396
                     /gene="flgN"
                     /locus_tag="ACHK3A_001262"
     CDS             36980..37396
                     /gene="flgN"
                     /locus_tag="ACHK3A_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO8739375.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(37436..38971)
                     /gene="murJ"
                     /locus_tag="ACHK3A_001263"
     CDS             complement(37436..38971)
                     /gene="murJ"
                     /locus_tag="ACHK3A_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO8739376.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(39081..40004)
                     /gene="yceM"
                     /locus_tag="ACHK3A_001264"
     CDS             complement(39081..40004)
                     /gene="yceM"
                     /locus_tag="ACHK3A_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO8739377.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVRAQRIVDKIWRDAMSE"
     gene            complement(40006..40653)
                     /gene="yceH"
                     /locus_tag="ACHK3A_001265"
     CDS             complement(40006..40653)
                     /gene="yceH"
                     /locus_tag="ACHK3A_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO8739378.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVMDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(40664..41248)
                     /gene="rimJ"
                     /locus_tag="ACHK3A_001266"
     CDS             complement(40664..41248)
                     /gene="rimJ"
                     /locus_tag="ACHK3A_001266"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO8739379.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            41484..42692
                     /gene="mdtH"
                     /locus_tag="ACHK3A_001267"
     CDS             41484..42692
                     /gene="mdtH"
                     /locus_tag="ACHK3A_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO8739380.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            42756..43403
                     /gene="grxB"
                     /locus_tag="ACHK3A_001268"
     CDS             42756..43403
                     /gene="grxB"
                     /locus_tag="ACHK3A_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO8739381.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPIELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            43537..44097
                     /gene="yceB"
                     /locus_tag="ACHK3A_001269"
     CDS             43537..44097
                     /gene="yceB"
                     /locus_tag="ACHK3A_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8739382.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            44203..45249
                     /gene="pyrC"
                     /locus_tag="ACHK3A_001270"
     CDS             44203..45249
                     /gene="pyrC"
                     /locus_tag="ACHK3A_001270"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO8739383.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVRKEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            45323..45568
                     /gene="dinI"
                     /locus_tag="ACHK3A_001271"
     CDS             45323..45568
                     /gene="dinI"
                     /locus_tag="ACHK3A_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO8739384.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            45858..46112
                     /gene="bssS"
                     /locus_tag="ACHK3A_001272"
     CDS             45858..46112
                     /gene="bssS"
                     /locus_tag="ACHK3A_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO8739385.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            46227..47345
                     /gene="solA"
                     /locus_tag="ACHK3A_001273"
     CDS             46227..47345
                     /gene="solA"
                     /locus_tag="ACHK3A_001273"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO8739386.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            47393..47506
                     /locus_tag="ACHK3A_001274"
     CDS             47393..47506
                     /locus_tag="ACHK3A_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO8739387.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVYYF"
BASE COUNT        11526 a        12793 c        11840 g        11436 t
ORIGIN      
        1 cttcactatt gagaataaca tgaatggtgc attggtcata ccaaaattga tgttaatcaa
       61 gttttgttgc tgaaaagata ggcgacttta ccgggcgaga tcaaaaaaca gagtttattg
      121 tttattattt gagcgaattt gtgagagtga tatagagtga ggggtgtatg gagtgcgttc
      181 ggtaggcctg ataagaggcg catcaggcat tgatgctcga ctgtcggatg cggcgtggac
      241 gccttatccg acttacagaa ttcggagcgc atcaggtacc ggcaaggtta cctgatgcga
      301 cgagggcatt tacccgaggg ttttgaacgc cttaatgcgc tgcaaatgcg gggagatatt
      361 cttgaatttg tgcgtctgct cttcgtccca gacaatctcg taataatgat gcagctcttc
      421 agatgtccgc tggctgttaa gagcttcatc attacgcgac aaaatcacca aacaacgatc
      481 gcgattcttt tcgcggaagt tggtcacaca cttagtggca atatcggcat actcttccgg
      541 gcgatcaatc ttgccttcca tgttctcata agggaacaaa ttagggttga agatcacctg
      601 gcggatatcg caaagaaaac caatccgttc cgcccagtat ccgcccagac caacgccgca
      661 aatcagcgga cgctcgtcga cgttcagttg caacattttg tccacttctt tgagcagatg
      721 ctgcatatca tgtttcggat gccgcgtact gtagcttatc aagcgtacat ccgggtcaat
      781 aaactgcaat tgtaagactt tctcgtggtt acccggactg ttagagtcaa aaccgtgtaa
      841 atagataatc atcgcttcct cacataagcc agggttagtg acctgctttc tcttcctgcc
      901 agcgttcatg taactgattc agacgggcgc tgatcgcttt ccagcgagcc gagtccatca
      961 gttcctgtcg cgaaaatgaa cctttatgat acaaacgtgt aacacgttct gccttgatcg
     1021 gtgacagatt atctaacacg ctgacggccc ctttacgatt attgcagacc aggatcatat
     1081 cgcaacccgc gtccagtgat gcctgcccgc gttcggcata actgcccata atcgcggctc
     1141 cttccatcga taaatcgtca gagaaaatca cgccatcaaa acccagttcc tgacgcaaaa
     1201 cggttttcag ccaataggga gaaccgctcg ccggacgcgg atcgacatca ctgtagatca
     1261 catgcgcagg cataatggcg tcgagtttat tttcgcggat taaggaactg aagaccgaca
     1321 tatctttagc gcgaatctcc gcttgcggac gtgggtcgca cggtgtttct ttgtgtgagt
     1381 ctgccgttac tgcaccgtgt cctgggaagt gtttcccggt cgttttcatt ccggcttcat
     1441 gcataccatc aataaaccgg ctggcaattg ccagggcttt ttcgggatcg gaatgataag
     1501 aacgctcgcc aatcgccgcg ctgatatgcc cgacgtccag cactggcgca aagctgatat
     1561 caatatccat agcaatcatt tcgctggcca tcaaccaacc tgcctctcgc gccagttttc
     1621 cgccttcttc cattcccaac agcgcagcaa atgattgtgc cgctggcaag cgggtaaaac
     1681 cttcacgaaa acgctgcacg cgtccacctt cctgatcaac cgccaccacc agatgattgc
     1741 gcgaagctgc gcggatctgg cgcaccagtt cacgtaactg ggcaggatca tgatagttac
     1801 gcgtaaagag aatcagccct cccaccagcg gatgcgccag tatttcacgc tcttccgcgt
     1861 ccagttcgta accttcgaca tccaacatta ctggacccac actgctctcc ttattgtttt
     1921 attaatagct gccgccaggt gtcatcggcc agcctgataa attgttgatc gccggtttgt
     1981 cgccagcggt actcaaacca ccctgctttg agcatcagca gccagggaaa ccatcgcctg
     2041 acctgacgcc ataattgcgc cggataaatc ttcgcgcgag tggcatagtc attgaccaat
     2101 tgccggtgct ggtcagtatt ttccacccac accgccgcca gttccagcgc gatatcacca
     2161 tctccggcat actcccagtc gatgagtttt aaccctgacg cgctatgcac taaatttccg
     2221 gcgtggacat ccatatgcaa tggacttaag cgtaaaggcc gtggttcccg cgctttgcgc
     2281 agacgtttta accttcgcag ccaacccact gtccgccgcg ccggatcgct ttgctgccag
     2341 tacagttcca gtaacggcaa cagcgttatt cgccagccaa aacgcggttg ttgatgtaga
     2401 taatacagca agcctgccag ttcgttggta tccggcaaat acgtttttac ctcgccgggc
     2461 agatagtcga ccaccatcca gtcacggaga tataaatgcg gcttcggtgc aatgcatgcg
     2521 ggtagttgtg ataaagcccg atactggcgc aagaacgcga accgcggcgc atcaggatcg
     2581 tgcggctgac gcacaacaaa acgctggccc tgatgttcaa tgagaaaact cccgccacta
     2641 agcccactat taaacgtcgt gacgggatga aactgcggga aaaagcgcga cagcaattcg
     2701 tcgcgcgtga tgggattatt gctgcgaaac ggcaccttta cctgaccaga taatttcgcc
     2761 cgtctgcacc agcatcagct gcatttgaag ggtcggagcg ttaacattgc cagaggcgct
     2821 ggagtacagc acgtaatgag cgccgacatt gcgggcaatg cctatggctt tactacgggt
     2881 gcctaaactg tcctgcggcg acaaacctaa ctgttgcttt gccatcgaca gctgctgggc
     2941 ggaaaccagg gtaaatttac cgttattagc cagcgcattt cgcagcgttt cggtcgcttc
     3001 tgcggcattc agcgaaccgt tagtacggtt gttaacgcta tcaaccagca ggacgctacc
     3061 cgcagtcacg ccgtcagccc caagcatctt actgaccatc ggctgcattg cgccattcca
     3121 gtcataatgg cgaatatgcg gcgcaggcgg tgcagtttga tcttcgtgct caattgggcc
     3181 tggctgctgc ggtatcgtcg gcaccgatgg cactgtgggg acaggctgtt gcggctcggc
     3241 tggttgttcc ggcgctggtt tcacttcttc taccggcgca ggttcacgtt gccccacaca
     3301 cccggcgaga aacatcgcca gcgcggtaat caaggcgtag cgactcattt tggtcatcaa
     3361 gattcacccc ttacaaatat agataaagtc tgactttgtg cgcccccaga aaattggcgc
     3421 tgccgtacag cgttaccgcc gaatgtgcgg gaagggtaac gctgcgtggc ctttccagag
     3481 gatgcatctc cagccctctg gcgtcatacc agtaaaaacg ataatgaacg gtaacgggtt
     3541 cttgcctttc gttataaagc gttgaggatg ctgaaggttg aatatcagac gtcgaaagga
     3601 cgggcttttc tgcactgatg cccgcagcca ataaagatga ctccatcacc agcgattgct
     3661 catcattcac cggaatttcc ggatgtgaac gacagcccac cagcactaac aacgccagag
     3721 acaccagccc aaagcatcct tttctcattg ttacagacct ttatgcgcca gcattggtcc
     3781 cagtggacgg ccacccaaca aatgcatatg gatgtggtaa acctcttgtc cgccatggcg
     3841 gttggtgttc atgatcaggc gatagccatc ttcggcaata ccttcttgct cagcaatttt
     3901 tgccgctacg gtgatcatgc gtcccagcgc ctgctcatgc tcagctgaca cgtcgttcac
     3961 agtcggaatg aggatattcg gaatgatcag aatatgcgtt ggcgcttgcg gcgaaatatc
     4021 gcgaaacgcc gttaccagat catcctggta gacgatatcg gaggggatct cacgacgaat
     4081 aattttgctg aatatagttt cttctgccac gacattttcc tttttcaaat agccatgcgt
     4141 tgcgccatgc tacgccgcgc cacactgcga gtatagagta tggtcgagtt aaacctgctc
     4201 tttcaacctt aactcacgat ttcttaagca aaaaatcgtt ttgctgccgt atatatcgcc
     4261 attattccca tttctgcctg cgaatgcgct gaaaagacat tctctgtttg gtttacacgt
     4321 caaatatgaa tgcgtatatt tctcatttgc atttacatgc aaaattattc gcacactaaa
     4381 tataaccgct catcaccctc ctccggcatg agcctgacga caacataaac caagagattt
     4441 cagatgcttt caacacaatt taacagggat aatcaatatc aaaccacctc caaaccgtca
     4501 ctgcttgccg cctgcatagc actggcactc ttaccttccg ccgcttttgc tgcaccagcc
     4561 actgaagaaa cggtgattgt tgagggttca gccactactc cagttgatga cgaaaatgat
     4621 tacagcgtaa cgtctacctc tgcgggtacc aaaatgcaga tgactcaacg tgatattcct
     4681 cagtcggtca ctattgttag tcagcagcgg atggaagatc agcagttaca aacgctgggc
     4741 gaagtgatgg aaaacacgct ggggatcagc aaaagccaag cggattccga tcgtgctctt
     4801 tattattccc gcggattcca gatcgataac tatatggttg atggtatccc cacctatttt
     4861 gaatcgcgct ggaatctggg cgacgcactt tctgatatgg cactgtttga acgcgtagaa
     4921 gtagtgcgtg gcgcgacagg actcatgacc gggacgggta atccatctgc ggcaattaat
     4981 atggttcgaa aacacgcgac cagtcgtgaa tttaaaggcg atgtctcggc ggaatacggt
     5041 agctggaaca aagaacggta tgtggcggat ttacaaagcc cactcaccga agatggtaaa
     5101 atccgcgcac gaattgtcgg cggctaccag aataacgact catggctgga ccgctacaac
     5161 agtgaaaaga ccttcttctc cggcattgtc gatgctgatt taggcgatct tacgacgctt
     5221 tcagccggtt acgaatatca gcgcattgat gttaatagcc caacctgggg cggtttaccg
     5281 cgctggaata ctgatggcag cagcaacagt tacgatcgcg cacgcagtac cgcacctgac
     5341 tgggcgtata acgataaaga gatcaacaag gtctttatga ccctcaagca gcggtttgct
     5401 gatacctggc aagcgacact gaatgccacc cactccgaag tcgaatttga cagtaaaatg
     5461 atgtatgtcg atgcctatgt aaacaaagcg gatggtatgc tggttgggcc atacagtaat
     5521 tatggacctg gctttgatta tgtcggcggt accggttgga acagcggcaa acgtaaagtt
     5581 gatgcgctgg atttgtttgc tgacggtagt tatgaattgt ttggtcgtca gcacaatcta
     5641 atgtttggtg gcagttacag caaacaaaac aatcgttact tcagttcatg ggcctacatc
     5701 ttcccggatg aaattggcag tttctacaac tttaatggca atttcccaca aaccgactgg
     5761 tcaccacaga gcctggcgca ggacgatacc acacatatga aatcgttata tgctgccact
     5821 cgtgtcaccc ttgccgatcc gctgcatctg atcctcggcg cacgttatac caactggcgg
     5881 gttgatacgc tgacttacag catggagaaa aaccacacca cgccttacgc tggtctggtg
     5941 tttgacatca atgacaactg gtcgacctac gccagctata cctctatttt ccagccgcaa
     6001 aatgatcgtg acagttcagg caaatatctg gctccaatca ccggtaacaa ctacgagctg
     6061 ggtctgaaat cggactggat gaatagccgt ctgaccacca cgttagccat cttccgtatt
     6121 gagcaggata atgtcgctca gtccaccggt acacctatcc ccggcagcaa cggcgaaacc
     6181 gcctataaag cggtggatgg gacagtcagt aaaggtgtgg aatttgaact caacggcgca
     6241 attaccgaca actggcagct gacgtttggc gcaacgcgct atattgcaga ggataacgaa
     6301 ggaaacgccg ttaatcctaa tctgccacgc accacggtta aaatgttcac cagctaccgg
     6361 ttgcctgtca tgccagagtt gacggtcggc ggtggtgtta actggcaaaa tcgcgtgtat
     6421 accgacaccg tgacaccgta tggcaccttc cgcgccgagc aaggtagcta cgcgctggtg
     6481 gatctcttca cccgctacca ggtgacgaaa aacttctcgt tacaggggaa tgtcaataac
     6541 ctgttcgaca aaacctacga taccaacgtg gaaggttcta ttgtctacgg cgcaccgcgt
     6601 aatttcagca ttaccggcac gtatcaattc tgaataacac ctgtaaaaaa ggcagccatc
     6661 tggctgcctt agtctcccca acgtcttacg gattagtggt tacggatgta ctcatccatc
     6721 tcggttttca ggttatcgga tttagtaccg aaaatcgcct gaacaccaga accagcaacc
     6781 actacgcccg ctgcgcccag tttcttcagg ccagcctgat ccactttaga cacatcagca
     6841 acgctgacgc gcagacgggt aatacatgcg tcgaggttag taatgttttc tttaccacca
     6901 aatgcagcaa ccagagccgg tgccatttcg ctggtacctg tcgcttttgc gtcttcagtc
     6961 gcgtcttcac gacccggcgt tttcagatcc agtgctttaa tcagcacgcg gaagatggtg
     7021 tagtaaacaa tggcataacc gataccgacg atcgggaaca gccacagttt gctgctgtta
     7081 ccagacagaa cgatgaagtc gatcagaccg tgcgagaacg acgtaccgtc acgcatcccc
     7141 agaagaatac agattgggaa tgccaggcct gccagaatcg cgtggatgat gtacaggatc
     7201 ggcgcaacga acatgaagga gaactcgatc ggctcggtga taccggtcag gaacgaggtc
     7261 agcgccgcgg agatcataat accgcccact ttcgcgcggt tttctggttt agcagagtgc
     7321 cagatagcaa ttgcggcagc tggcagaccg tacattttga acaggaagcc accagacagt
     7381 ttacccgcag tcgggtcacc cgccatataa cgcggaatgt cgccgtggaa aacctgacct
     7441 gctgcgttgg tgtattcacc aatctgcatc tggaaaggta cgttccagat gtggtgcaga
     7501 ccaaacggta ccaggcaacg ttcgatgaaa ccgtaaatgc caaacgcaac taccgggttc
     7561 tggtaagcag cccactgaga gaaggtctgg attgcagaac caatcggcgg ccaaatgaag
     7621 gacagcacaa cgccagtaaa gatggcagcc aggcctgaaa tgatcggcac aaagcgttta
     7681 ccggcaaaga agccaagata ctcaggcagc ttaatacggt agaaacggtt aaacatgtac
     7741 gctgcgatcg caccggagat aatccccccg agtacgccag tatccgccag gtgtttagag
     7801 gcgatttctt cagcaggtaa atgcagtacc agtggcgcaa ccacggccat ggttttaacc
     7861 atgatgccat aggcaacaac tgcggccagc gcggatacgc catcgttatt ggtaaagccg
     7921 agggcgacac cgatcgcaaa aatcagtggc atgtttgcaa agacggaacc gcctgcttct
     7981 gccataacat gcgatacaac ggcgggcagc cagctgaaat tcgcggaacc gacgcccagc
     8041 agaatacctg cgataggcag tacggatacc ggcagcatca gcgatttacc gaccttttgc
     8101 aggttagcaa atgcattctt aaacataatt gagagtgctc ctgagtatgg gtgctttttt
     8161 tacgttttca cgcgtggcaa ggggggagag cctcgccgtg tacagggcat ctaagcgccc
     8221 tttatttatt acacagagta aaataattca gtgccgattt gtttgacggc tatcacgttt
     8281 caacttcaga tcattggaga agttgcacat tttgacaaaa catatgtcac aatatttcaa
     8341 acgtggttat tcaccgccca attttaggcg gtgaacttta ctcgttttag ccattaatta
     8401 cgagctttaa aaaaactcat tcaacggatg gattgaaggc gggaagcgtc gatgtgaaac
     8461 agacgggcga agttatcggt ggttacctgc gccagttctt caacggcaac acctttcaac
     8521 acagccatgt attctgcaac gtcacgaacc atcgcaggtt gattctcttt tcctcgatgc
     8581 ggaaccggtg caaggtaagg tgagtcagtt tctaccagta accgatcctg ggggacataa
     8641 cgcgcggcat cgcgcagttg ctccgcatta cggaaggtca caatgccgga aaaggagatg
     8701 taaaatccga gatccagtaa tttacccgcc gtttctctgt cctctgtaaa acagtgtagt
     8761 acgccaccgc aatccgtcac tttttcttcg cgcagaattg ccagcgtatc ggcgcgggcg
     8821 tcacgggtat ggacgataac cggcttgttc agttcacgac caatctggat atgatggatg
     8881 aaggattctt gctgccgtac tttagtttcc ggcgtgtaat aataatccaa cccggtttca
     8941 cccagcgcaa caacgccctc ttctgccgcc agacggcgta aatcttctac atcgtaggga
     9001 tcattctggt tgagtggatg cacgccacaa gaaaatacga cgttgtcacg ttcgcctacc
     9061 agatcccgca tgtgtaaata acccggtaat gttgtggcga ctgccagaca aaatttcaca
     9121 tcgcgtgcgg cggctttcgc cagaacgtca tccacgtcct tatgcaaaga ttcataatcc
     9181 agaccatcga gatggcagtg tgagtcgact aaaaacatga tgtctctctt aaagatgagg
     9241 aaccggtagc acaacgcccg gttgcaggta atgctcaata cgcagtaaaa gatcggtgat
     9301 gagaagctcg cggttaatac ctgtgacaga cattaactgt tcacgaatgt ggcaaacatc
     9361 ccccagtata gcctgcaggc gcgagggaga aagatggttt gccagttcga cgaccaggcc
     9421 cggcacatca acattggtca cctgcgcagc gccatgatgg cgttttagcg catccatcag
     9481 caacgttgcc agccagtgta aacgcgccgg agcttgttca tgattaaggg ccgctagcag
     9541 cgaataccag tcgcccgatg gcacgctata tgccaaagcc tgacacaatg tttcacgagc
     9601 ctgccagtta tctccgtgaa acaacgccag tgccgcgcca ggcgaaccgg cacttaagcg
     9661 caatgcggca agtaatgcat cctgtgacat tgtcacttcg cgtgaaagcc aggtcacggc
     9721 gtactgttcc ggcggcggcg caaggtaatg taaccgacaa cgactacgta atgttgccag
     9781 taaacgttct ggctcgcggg tagcaaggaa aaaccacgtt tctgctggtg gctcttcaag
     9841 cgttttcagc aatgcgttag ccgcggcgtc ggtcagtaag gcagcatcgg ttacccagac
     9901 aactttcgca ccacctaagc gtgcgtgctc attcagcttt tcggtgacct cacgtaccgc
     9961 atcaatgccc agcgtatttt tccctttttc gggagccagg gtgtagtaat cgggatgcgt
    10021 gccagcctgc atcaactgac atccacgaca gtgaccgcaa cttttgtggc cctgcggttg
    10081 ttggcagagt aaataacggc tcagggcgta gattaaagca tcatcgccca tgcccggtaa
    10141 cgcctgaatg agcagcgcat ggtgacctct tccggcctga tagctggcta ccagtttttc
    10201 gaaatcaggt cgtaaccatg gataccatct catgcgtcca actccttcac ccagttggtc
    10261 acggtagtgc ggattgcatc catcacagcc tccagcggca gggtggcatc aatggtatga
    10321 atgcttttat cttgtgctgc cagttccaga tagcgggcgc gggtgcgatt aaaaaaatcg
    10381 aaagactctt gctcaatacg atccagctcg ccgcgtgcac gcgcgcgttt taagccaact
    10441 tccggggtaa catcgagata gagcgttaag tcggggcgaa aatccccgag aacagcatca
    10501 cgcagtgttg ccagcatatg ttggtcaata ccacgtccgc cgccctgata cgcctgagtg
    10561 gagagatcgt ggcgatcgcc aatcacccaa gtgccgttag ccagcgcagg tttgatgacc
    10621 gtttctacca gttgaacgcg cgcggcataa aacatcagaa cttcggcttt atcggtaatg
    10681 acttcatcgc ctaccgattt gatatccagc accaggcttc ttaacttttc ggcaagttgc
    10741 gtaccgccag gttcccgagt gaaaaccatg tcgcggatac ccagttgctc aagcgtctca
    10801 accaccacgt tacgcgcggt agttttgcct gcgccttcca gcccctcaat gacgatatac
    10861 ttactgcgca tttttttcct taagcacttt cagataatcc tgcacagact tgttatgact
    10921 ggcaagattg gtattaaacg tgtgaccacc tttaccatcg gccacaaaat agagatacgg
    10981 cgtttttgcc ggatgcgcag cagccttcag cgaatccgcc cccggcgtcg ctatcgcacc
    11041 tggcggcaaa ccggtaatgg tataggtgtt atacgctgtc ggcgtttcca ggtctgcacg
    11101 agaaagtttg ccattataac gctctcccat cccgtaaatc acggtcgggt cggtctgcag
    11161 gcgcatacca atgcgtaacc ggttgataaa taccgaggca acctgatcgc gttcactggc
    11221 aacggcggtt tctttttcga taattgatgc catcgtcacc aactggtttt tatctttata
    11281 aggcagaccg tccgcacgcc cttcccaggc gctatcgacc gctttcacca ttttcttgtg
    11341 cgctcgcttg agtaacgcga catcggtggt attggcggta tacatccagg tgtctggcca
    11401 gaaccaacct tcaatccact ccgggttttc cagttcaagt gcctgcgcta cggtggcgta
    11461 cttatcgtcg ctcagcgtgt gcttgatata gggggcctca cgcaattgct tgaggtaatc
    11521 gctcagacgc atcccttcta ccagtcgcag agggaactgt gcttctttac cgctttccag
    11581 caatttcagc atctcgcgca cggtcatctg cggtgtaaag cggtaagtcc cggctttaaa
    11641 gtgagaaaga tccggttcga tacgcagcag ccattggaaa acccgtgggc gattgatgat
    11701 cttatcggca taaagctgtt caccgagcgc cagacgtccg gtccctggct tcagggtaaa
    11761 tatcgtctct tctttgataa gtaatttgct gtcggcaaga tggcgaagct tccagacgcc
    11821 cacaccagcg gcgataccca gtaccaccag caataacaag ataatcaata acactttttt
    11881 catgactaat tcgggcgctc acaaagtggg gctaaatatt catataacgt tgctgacgaa
    11941 aaggagacat cgccacaggc acgtacgggc atcactggca ttaacgcatt acaaataacc
    12001 atctcatctg cctgcaacgc ctcctccagc gaggcttgca cttcgacaag ctgataagag
    12061 gattgtgcca gcaaacggat acagaattgt cgcataatgc cgttaacacc tgcctgatcc
    12121 agtcgcggcg tataaactac gttgcccttc cgccagaaca aattagccgc acagcattcc
    12181 gtaacccacc cttcgctgtc aaggaccagc gcctcatcag cgtttgtctg ctcaagatga
    12241 gagcggatca atacttgctc aagccgatta agatgtttaa tacctgcaag atgaggattg
    12301 cgccccagcc gcaccgggct tagcgccaac gtcatcccct cgttacgcaa acggtcgtaa
    12361 tgtgcgggat aaaccgtaac ggagagaatc cgcgttgccg gtccgctgtt caatgtgctg
    12421 tatcctcgcc cgccactacc gcgactgatc accactttca gcacaccatt ttgctgttct
    12481 gctgccagcg ttttcatctc tagttcaagc tgaggccaga agtcacagga aatcatcaac
    12541 cgctgacaag catcctgtag tcgctggata tgcgccgata acaaactgac tttaccgtcg
    12601 ataactctgg cggtggtaaa acaaccatca ccaaactgcg ttgcccgatc gcttactgcc
    12661 agcgattcct gcttatgacc gttaattaag aacataccgg ctccttatca gagaattgtt
    12721 agtgtggcag catgttcact acggaacaag tcggaataaa agctaagaaa aaaggcccgc
    12781 aagcggacct tttataaggg tgggaaatga caacttagat ctttttaaag atcaaagaac
    12841 cattagtgcc accgaagccg aaggagttac acagcgtgta ttccattccg ctgacctgac
    12901 gtgcttcgtg cggtacgaaa tccagatcgc aaccttcatc cgggttatcc aggttgatgg
    12961 ttggcggaac agcctgatcg cgcagcgcca ggatggagta gatagattct actgcacccg
    13021 ccgcacctaa caggtgaccg gtcatagatt tcgtggagct taccaacaca cggcttgcag
    13081 cttcaccgaa gatggttttc accgcctgcg cttcagcttt atcgccagcc ggcgtagaag
    13141 taccgtgagc gttaacgtag ccaatctgac tcgcttcaat gcctgcatca cgcagagcat
    13201 ttgccattgc cagagctgcg cctgcgccat tttctggcgg tgacgtcata tgataagcat
    13261 cgctgctcat accaaagccg acgagttcag cgtaaatttt cgcaccgcgt tttttcgcgt
    13321 gttcgtactc ttcaagtacc agcataccgg caccatcgcc cagtacaaaa ccatcacgct
    13381 ctttatccca cgggcggctc gccgcttgcg ggttatcatt gcgggtagat aatgcacgtg
    13441 ccgcgccaaa accaccaacg cccagcggcg tactggcttt ctctgcgcca cctgcaacca
    13501 tcacgtcagc atcgccatac gcgataatac gcgcagcatg gccaatgttg tgcacgccgg
    13561 aagtacaggc ggtcgcgata gagatgcttg ggccacgcag gccatacatg atagtcagat
    13621 gacctgccac catgttcaca atcgttgacg gaacgaagaa tgggctgatc ttacgtggac
    13681 caccgttcat cagagatgtg tggttttctt cgatcagtcc gaggccgcca atcccggagc
    13741 caattgcggc accaatgcgg gttgcgttct cttccgttat ttcaaggcca gaatcctgca
    13801 tggcctgaac gccagcgaca attccatatt gaatgaaggc atccatcttg cgctgttctt
    13861 tgcgcgagat aatgtcctca cagttaaaat cctttactaa gccagcaaat ttcgttgcat
    13921 aggcgctagt atcgaaatgg tcgattaggc tgatgccact ctgaccggca agcagagctt
    13981 tccaggtaga ctctacggta ttgccgacag gagacaacat gcccagtccg gtcacaacta
    14041 cacgacgctt agacacgttt gtcctccagg gagggaaaaa atgattctag tgggacaaaa
    14101 agataaaact caggcggtcg aacgaccgcc tggagatgtt cacttacgcc tggtggccgt
    14161 tgatgtaatc aatggcagcc tgaacggtgg tgattttctc agcttcttcg tccggaatct
    14221 cagtatcaaa ctcttcttcc agagccatta ccagctcaac ggtgtcaaga gaatccgcgc
    14281 ccaggtcttc aacgaaagaa gcattgttgg taacttcttc ctgcttaacg cccagctgtt
    14341 cgccgataat tttcttaacg cgttcttcga tagtgctcat actcttaaat ttcctatcaa
    14401 aactcgcttt cgcgatggtt ttcgtagtgt ataaaatgtt gaaaaatttg caactaaatc
    14461 ccggcaggtc ttaccacgat tttacgttat tttgaggcct taccccccaa taacgcaaat
    14521 cattttgcac taattattac tcataaccac gcagactgcg cgcaacatga gcattttgtg
    14581 caaatcgcgg tcagaccatg tacatcccgc cgttcacatg caaagtttca cccgtgatgt
    14641 aagctgcttc gtcggatgcc aggaatgcaa ccgcgttggc gatttcctgt gcgccgccga
    14701 ggcgacccgc aggaacctgc gccaggatac ccgcacgctg gtcatcgctc agcgcacgtg
    14761 tcatgtccgt ttcaataaag cccggagcaa caacgtttac agtaataccg cgtgacgcaa
    14821 cttcgcgcgc cagtgattta ctgaagccga tcaagcccgc tttcgccgca gcgtagttgg
    14881 cctgaccgcc atttcccatg gtaccaacca cagaaccgat agtgataata cgaccatgac
    14941 gctttttcat catagcgcgc attaccgctt ttgacagacg gaaaacagat gaaaggttgg
    15001 tttcgataat atcgttccac tcttcatctt tcattcgcat taacaggtta tcacgagtga
    15061 taccggcatt attgaccagg atatccactt caccaaattc tgcgcgaatt ttttccagaa
    15121 cagattcgat agatgccggg tcggtcacat tcaacatcag acctttgccg ttggcaccta
    15181 aataatcagt gatcgcctga gcgccatttt cactggtcgc agtgccgata actttcgcgc
    15241 cacgggctgc gagcgtttca gcaattgcgc ggccaattcc gcggcttgca ccggttacca
    15301 gtgcgatttt tccttcaaaa ttcatgattt tcctctttta aagctcgagc gccgctgcca
    15361 tcgctgaagg ttcgttcagc gccgaggcgg tcagggtgtc gacaatgcgt ttcgtcaggc
    15421 cagtaagcac tttgcccggg ccgacttcat agagatgttc tacgccttgc gctgccatgt
    15481 attcaacaga cttcgtccac tgaaccgggt tatacaactg acgtaccagt gcgtcacgga
    15541 tggcatcacc attggtttcg catttcacat caacgttatt cacaacagga actgttggtg
    15601 cgttaaaggt gattttcgct aattctactg ccagtttgtc ggctgctggt ttcatcagcg
    15661 cacagtgaga cggtacgctc actggtaacg gcagagcgcg ttttgcgccc gcagctttac
    15721 aggcagcgcc agcacgctca actgcttctt tatgaccggc aataaccacc tgtcccggag
    15781 agttaaagtt taccggagaa acgacctgac cttctgcagc ttcttcacac gctttcgcaa
    15841 tagacgcatc atccagaccg atgattgccg ccatagcacc cgttccttcc ggtacggctt
    15901 cttgcatgaa cttgccgcgc atctcaacca gacgcaccgc atcagcgaaa tcaatcacac
    15961 cagcgcaaac cagcgcggag tattccccca ggctgtgacc agccatcatt gccggtgctt
    16021 taccgccctg ctgctgccat acgcgataca gcgcaacaga tgcagtcaac agcgctggct
    16081 gggtttgcca ggttttattc agttcttcag ccggcccctg ctgggtcagc gcccacaggt
    16141 cgtagcccag cgccgcagaa gcttcagcaa acgtttcttc gacaattgga tagctcgccg
    16201 ccatatcagc cagcattcca acggtttgag aaccctgtcc agggaacaca aatgcaaatt
    16261 gcgtcatgtt ttaatcctta tcctagaaac gaaccagcgc ggagccccag gtgaatccac
    16321 cgccaaaggc ttcaagcaga accaactgcc ccggcttaat gcgcccgtcg cgtacagctt
    16381 catccagagc gcacgggaca gaggccgcag aggtattacc gtggcgatcc agcgtcacca
    16441 cgacattgtc catcgacatg ccgagttttt tcgctgttgc actgataata cgcaggttag
    16501 cctgatgcgg aaccagccag tccagttgag aacggtcaag attattcgcc gccagcgtct
    16561 catctacgat gtgcgccagt tctgttaccg caaccttgaa gacttcgttg cccgccatcg
    16621 tcagatgaat tgaattctct ggattcacac ggtcagcatt aggcagcgtc agcaactcac
    16681 catagctacc gtcggcatgc agatgggtgg agatgattcc cggctcttca gaggcagcca
    16741 gcaccgcagc gcccgcgcca tcgccaaaaa taataatagt cccacgatcg gttggatcgc
    16801 aggtgcgcgc cagtacatcg gaaccgacga ccagagcata cttcaccgcc ccagatttca
    16861 cgtattgatc ggctacgctt aatgcatagg tgaaacctgc gcaggctgct gcaacgtcaa
    16921 atgccgggca acctttaatg cccagcatgc tttgaatctg acaagctgcg ctcgggaaag
    16981 cgtgcgtagc agaagtcgtt gccacaacga tcaggccaat ctggtctttc tcaatgcccg
    17041 ccatctcaat tgcgcgtgtc gccgcttcaa agcccatggt tgaaacggtt tcgtttggcg
    17101 cggcaatgtg gcgttcgcgg ataccggtac gagtgacaat ccactcgtca gaggtgtcca
    17161 ccattttttc caaatcggcg tttgtccgca cttgttcggg cagatagctg ccagtaccaa
    17221 taatcttcgt atacatgtac gctcagtcac ttttcggtta tataccgtca cttgcaaact
    17281 gcgagttcgc tggcagcgtc ctgctaccgc agagttccgc ttttgccacc gtccagcagc
    17341 tcaaaaccag ctgggtatac agattccagg cgagcggcaa ttcgctgagg aacttgtcgc
    17401 tgcaccgcct gcactgcctg ttcaatcgcg accgcaaaag ctcgctgatt ggctgcacca
    17461 tgacttttta tcaccgtgcc gcgcaatcct aacagacagg cgccgttata ctggtcgggg
    17521 ttgaggtgac tgaatcgcct cgtcaggctc ttttgtagcc aacgctttaa taacagtagc
    17581 caccacgacc gttttttccc ttcaccctga gatttcagca gagaaaggaa catcctgaca
    17641 acaccttcca tcgtctttaa tgtgacattt cctgtaaagc cgtcacaaac cagcacatct
    17701 gtcttgccag ttaacaactc attggcttca agatagccga tataattgat agaagggatt
    17761 gttttaagca ccgctgaggc atcccgaata ctgtcgagac cctttacttc ttcttcacca
    17821 atattgagca acgccacgcg aggattagga atttccacca cctcttcagc cagaactgag
    17881 cccataatgg caaattgcac caacattgta ctgtcacaat cgacgttggc ccctaaatcg
    17941 aggaccaccg ttttgccctt ttgctgatgc ggtaataccg tcaccagcgc cggacgctca
    18001 atcccctcca ggggcttgag taataatttt gccagcccca ttagcgcccc ggtattaccg
    18061 gcactgacac aggcttgcgc tcgaccttct ttcaccagct ccagtgccac gcgcattgaa
    18121 ctcccacgac tggcgcggat agcttgcgaa ggccgggcat cactggcgat aactgactgc
    18181 gcaggaataa tctgcagacg cgaacgttgt tcaaagtcag ctttagcaag taatggcgtg
    18241 atggcgtcgg gattgccgac taaaagaaga gtgagttgcg aattagaatt cagtgcctgc
    18301 aatgctgcag gcactgtcac ggaagggcca aaatcccctc ccatgacatc taacgccagg
    18361 gttagacgtg tcaaggtatc gtcgccgccc ggtttggtct ttccccagtt gcctggggaa
    18421 aatcctcact aagcttcatc acgcagacgc gtgattactt agcgatgacc ttgcggccgc
    18481 ggtagtaacc gtcggcagtg atgtggtgac gcaggtgttt ttcaccagaa gttttgtcta
    18541 cagacaggct ggtgactgcg gtcagcgcgt catgggaacg acgcatgcca cgtttggaac
    18601 gggttggttt attctgttgt acggccatgg accttactcc tcaattacgg tttatccccg
    18661 atccaacggg gagcaccaat tacttacgct ttaagctggc taatacggca aatgggtttg
    18721 gcttttgcgc ttcttcaggc agttcaccaa agaccatgtc cgcttcggac acttcacagt
    18781 gttcagaatc atgcaccgga actaccggca aggcgaggat gatttcatct tcaaccattg
    18841 caagcagatc gatttcaccg aattcgttaa cctcaatcgg ttcatacgct tccggcagtg
    18901 cttcagcctg ttcgtctgaa cgcacaggac taaaacaata cgttgtgtag acctgatgag
    18961 taaacggctt cccgcaacgc tgacactcga gcgttaccgt caccttcgca tcgccgttta
    19021 acactgcgag acgttggtta tcgatagcga acgacatgga gcattccaca tcactgtcca
    19081 cactgactac ggattcggcg acgcgctcaa cctgatcagg agtatagata ccctggtaat
    19141 caaggcgttt ttgagccgta cgaaccggat cgagagtcag gggtaatttt accttttgca
    19201 tagggcgcgc atattaactt tgtaacgtca tagagtcaaa gaaaaaggca gcctgtggtt
    19261 gccttttgcc aataattcgc acacattgcg ggttactgct ttattttcgt tcagtgctga
    19321 taacgaacat caacctgcaa aacgaagcgc atagtttaaa atggctatca tcaatacgct
    19381 atatctgatg gaaaaaaata tgcctaaact tattttagcc tccacctcgc cctggcgtcg
    19441 cgctcttctg gaaaaactgc aaatctcttt tgaatgtgca gcacccgagg tcgacgaaac
    19501 tccacgcagc gacgaatcac cgcgacagtt ggtgcttcga ctggcacaag aaaaagcgca
    19561 atctctggcg tcacgttatc cggatcattt aattattggt tcagaccagg tatgtgttct
    19621 tgatggcgaa atcaccggta aaccgttaac ggaagaaaat gcccgtctgc aattacgcaa
    19681 agccagcggc aatatcgtca ccttctatac tggactggcg ttgtttaatt cggcgaatgg
    19741 gcatctacaa acagaagtgg agccttttga cgtccatttc cgtcacctga gcgaggcgga
    19801 gattgataat tacgtgcgta aagagcatcc cctgcactgc gcgggtagct ttaagagtga
    19861 aggatttggc attacgctgt ttgagcgttt agaggggcga gatcctaaca cgctggttgg
    19921 tttaccgcta atcgcgctgt gtcagatgtt acgtcgggaa gggaaaaacc cgctgatggg
    19981 atagttttct gcgccggatg cggttacgtc gcatccggca atcggtgctg gcggcctgat
    20041 gcggcgtaaa cgccttatca aggccaaccg cccattatca ggtttatatg cttgtattag
    20101 cgcgcgttac gcagcttttg caaacaacgc ttcaaacctt catccatcgg cgcttcgata
    20161 cgcatcacct caccggtccc cggatgggta aacttcaacg ctgcggcgtg caggaacaga
    20221 cgatttaatc ctgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc accgtagcga
    20281 tcgtcaaagg caatcggatg acccgcatac tgtgtatgca cacggatctg atgagtacgc
    20341 cctgttaccg ggctacaacg caccagggtg gcaaaagcat agcgttcttc cactttaaag
    20401 cgtgtttccg acggtttgcc ttcctgactc acacgcacga tacgttcgcc gctttgcaga
    20461 atatttttca gcaacggcgc ttgaacgctc ttcacatgcg actgccactg accgcgcacc
    20521 agcgccaggt aatctttttg catccctttt tcacgtaatt gctcatgcag agaacgcaac
    20581 gccgagcgtt ttttcgctac cagcaacaca cctgaagtgt cccggtcgag acgatgaacc
    20641 agttcaagga accgcgcttc cgggcgcaac gcccgcaaac cttcgataac gccgaagctt
    20701 aaaccactgc cgccatgtac cgccgtaccg gaaggtttat tcagcaccag gatatggtca
    20761 tcttcatata agatgacgtc cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc
    20821 tcttcttccc gttcagcaac gcgaaccggt gggatacgca cctcatcacc cgcttcgagt
    20881 ttatattcag gcttaatacg ttttttgttc acccgcactt cgcctttacg caaaatacgg
    20941 taaatcatac tttttggtac gcctttcaat tgggtacgca aaaagttatc gatacgttgc
    21001 cccgcttcgt cagcgttgat agcaacaatt tttacggatg gagtctctgt tttcatggtg
    21061 ggcgattcta attagccaac aggattcgcg ccactcattt ttctatgctt atatttagtt
    21121 ttgcacctta ttacttcact acgtgatcac tttattgatg gttattaaac caatcaccag
    21181 caagaagtga aaaaactgtg agtaagcggg tgataaatgg taaaagtcat cttgctataa
    21241 caaggcttgc agtggaataa tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat
    21301 tttacggaat aacccatttt gcccgaccga tcatccacgc agcaatggcg taagacgtat
    21361 tgatctttca ggcagttagc gggctgcggg ttgcagtcct taccggtaga tggaaatatt
    21421 tctggagagt aatacccagt ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa
    21481 cgggcaaccg acactctgcg cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga
    21541 ctagacacga ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg
    21601 tcaatgtaag aataatgagt aagttacgat gaaaagaatg ttaatcaacg caactcagca
    21661 ggaagagttg cgcgttgccc ttgtagatgg gcagcgtctg tatgacctgg atatcgaaag
    21721 tccagggcac gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc
    21781 gagtctggaa gctgcttttg ttgattacgg cgctgaacgt cacggtttcc tcccactaaa
    21841 agaaattgcc cgcgaatatt tccctgctaa ctacagtgct catggtcgtc ccaacattaa
    21901 agatgtgttg cgtgaaggtc aggaagtcat tgttcagatc gataaagaag agcgcggcaa
    21961 caaaggcgcg gcattaacca cctttatcag tctggcgggt agctatctgg ttctgatgcc
    22021 gaacaacccg cgcgcgggtg gcatttctcg tcgtatcgaa ggcgacgatc gtaccgaatt
    22081 aaaagaagca ctggcaagcc ttgaactgcc ggaaggcatg ggacttatcg tgcgcaccgc
    22141 tggcgtcggc aaatctgctg aggcgctgca atgggattta agcttccgtc tgaaacactg
    22201 ggaagccatc aaaaaagccg ctgaaagccg ccctgctcca ttcctgattc atcaggagag
    22261 caacgtcatc gttcgcgcat tccgcgatta cttacgtcag gatatcggcg aaatccttat
    22321 cgataacccg aaagtgctcg aactggcacg ccagcatatc gctgcattag gtcgcccgga
    22381 tttcagcagc aaaatcaaac tgtacaccgg cgagatcccg ctgttcagcc actaccagat
    22441 tgagtcacag attgagtccg ccttccagcg tgaagttcgt ctgccatctg gtggttccat
    22501 tgttatcgac agcaccgaag cgttaaccgc catcgacatc aactccgcac gcgcgacccg
    22561 cggcggcgat atcgaagaaa ccgcgtttaa caccaacctc gaagctgccg atgagattgc
    22621 tcgtcagctg cgcctgcgtg acctcggcgg cctgattgtt atcgacttca tcgacatgac
    22681 gccagtacgc caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg
    22741 ggcgcgtatt caaatcagcc atatttctcg ctttggcctg ctggaaatgt cccgtcagcg
    22801 tctgagccca tcactgggtg aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg
    22861 taccgtgcgt gacaacgaat cgttgtcgct ctctattctg cgtctgatcg aagaagaagc
    22921 gctgaaagag aacactcagg aagttcacgc cattgttcct gtgccaatcg cgtcttacct
    22981 gctgaatgaa aaacgttctg cagtgaatgc gattgaaact cgtcaggacg gcgtgcgctg
    23041 tgtgattgtg ccaaacgatc agatggaaac cccgcactac catgtgctgc gcgtgcgtaa
    23101 aggggaagaa actccaacct taagctacat gctgccgaag ctgcatgaag aagcgatggc
    23161 gctgccgtct gaagaagagt tcgctgaacg taagcgtcct gaacaacctg cgctggcaac
    23221 ctttgccatg ccggatgtgc cgccagctcc aaccccagct gaacctgccg cgcctgttgt
    23281 agccccagca cctaaagctg caacggcaac accagcatct cctgcacaac cagggttgtt
    23341 gagccgcttc ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaaa ccaaaccggc
    23401 cgagcaacca gcaccgaaag cagaagcgaa accggaacgt caacaggatc gtcgcaagcc
    23461 tcgtcagaac aaccgccgtg accgtaatga gcgccgcgac acccgtagtg aacgtgctga
    23521 aggcaacgat aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc agcagactgc
    23581 cgagacgcgt gagagccgtc agcaggctga ggtaacggaa aaagcacgta ccaccgacga
    23641 gcagcaagcg ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac gtcaggcgca
    23701 acaagaagcg aaggcgctga atgttgaaga gcaatctgtt caggaaaccg aacaggagga
    23761 acgtgtacgt ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta
    23821 cgagcaaagc gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa ctgtcgctgc
    23881 cgaaccaatt gttcaggaag cgccagctcc acgcacagaa ctggtgaaag tcccgctgcc
    23941 agtcgtagcg caaactgcac cagaacagca agaagagaac aatgctgata accgtgacaa
    24001 cggtggcatg ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg
    24061 tcgtcgtcgc tatcgtgacg agcgttatcc aacccaatcg ccaatgccgt tgaccgtagc
    24121 gtgcgcgtct ccggaactgg cctctggcaa agtctggatc cgctatccaa ttgtacgtcc
    24181 gcaagatgta caggtcgaag agcagcgcga acaggaagaa gtacaagtgc agccgatggt
    24241 gactgaggtc cctgtcgccg ccgctgtcga accggttgtt agcgcgccag ttgttgaaga
    24301 agtggctgaa gttgtagaag cccccgttca ggttgccgaa ccgcaaccgg aagtggctga
    24361 aacgacgcat cctgaagtaa ttgccgccgc ggtaactgaa cagccgcagg tgattaccga
    24421 gtctgatgtt gccgtagccc aggaagttgc agaacacgca gaaccggtgg ttgaaccgca
    24481 ggaagagacg gctgatattg aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc
    24541 tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac
    24601 ggtagccgcg gttgaaccag agatcaccgt tgagcataac cacgctaccg cgccaatgac
    24661 gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt cacagtgact ggcagcgccc
    24721 tacttttgcc ttcgaaggca aaggtgccgc aggtggtcat acggcaacgc atcatgcctc
    24781 tgccgctcct gcgcgtccgc aacctgttga gtaataatta gctcaagtaa tcaagccctg
    24841 gtaactgcca gggctttttt atttcagggg tagcatcttt gctgtaatga cattagcgtt
    24901 acatgtactg tataattatc atccacgcaa agccgaagga tatatttatg ccagcagcca
    24961 ataacattcc agccaggcgc gaatctttaa acttgcgcat aaaacccgca gaacgcgatc
    25021 ttattgaccg ggccgctaaa gcccgaggaa aaaatcgcac cgaatttgtc cttgaggccg
    25081 ccagagccgc agctgaagag gcgctcattg agcagagaat tttcaggatc gattctgatg
    25141 attatcagct atttttgaat cgtctggatc agccccccgc gcccaatgct gcattacgca
    25201 aaaccatgca aacacccgcg ccgtgggagc aaaaaaaatg attcaggccc ccgagccact
    25261 catggcaagg catcaattca cctctttttg ctcgggagtg gaaacgatgg ataactggct
    25321 aaagcagaga gccctaaaaa atcagcttgc cggcgcatca cgtacatttg ttagctgtga
    25381 tacgtatagc aacgttttgg catattattc gcttgcttcc agcgccgttg aaacgtatgt
    25441 cgcgacagga cgatttcgcc gaaatatgcc agaaccgatc cctgttgtag tacttggtcg
    25501 tctggcaata gataaatcat tacagggtca gggcatcgat cgtgcgatgg tcagagatgc
    25561 cggtctacga gtactacagg cggctgaagt cattggtatt cgcggaatgt tggtccatgc
    25621 gttatcagac caggcccgtg agttttatct gcgagtgggg tttgaaccat cgcctgtcga
    25681 ttctatgata ctgatggcaa cattggctga tttgcaggaa tgtcttaaat agttcgttat
    25741 gtctcatgca aaaaaatgcc cgccccggcg atcccagagc aggcagacaa aacataccca
    25801 gtttcatgat atgttttaaa gcgaaattat ttgttgagct ggaagagcga caatccctgc
    25861 atatcggtaa atgctttata cgacgcctgc aatgccgttt gctgcatgat gtaagatgaa
    25921 atagttgcat tccagtccac atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg
    25981 cgatcgctac ctaatgaatc cagcgactcc agttcgttca gctgcgtgcc taattccgcg
    26041 cgaacagtca gcacattgtt cagcgagttt ttcagtccgc ggttggtttt atccagtgcc
    26101 gcagcggcgg tttctttatc cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg
    26161 gcgctatcca gcatggcaaa aagattggtt tcagaagcgc taccgtctgg ttccgctacc
    26221 gcgttgctgg taatactgtc gaaaattttg tcacccgtgt gccctatcac catcgaacgc
    26281 gaagcatcga cctgttgttt aatactttct gctccgcctt cgtattttcc atccgcctcg
    26341 ctaaacggtg cggattctgt tttataaccg gcaaaaatgt agcgcccgtt accgtcagtg
    26401 gtgttcgcca gattcagcaa ctggtcacga agcccctgaa tatccgtagc cagcgaggcc
    26461 cggtcatcgt cactcaaggt gccattgctg gcgtagacaa ttttttcctg agcattctgg
    26521 atagcggtgg tgacctggct aagtacactc tcttccagtg acactttttg agtggcgaaa
    26581 gtacgcgcca gcgtgtactg gctgttttgc gcctgtgcct gggagagaac aacagcttgt
    26641 gatgcagcaa tgggatcatc tgaagggtta acgactcgct tacccgtcga catctgttcg
    26701 ccgtacttca tccattctgc ctgagaattg gtgataccac gcatgttttg ctggtacatc
    26761 atctgggtac tgaaacgcat cttatctccc cttagcgaat gttaatcagc gcatcaaaaa
    26821 tcgcgtttgc cgtctgcaga acctgcgcat tcgccaggta atactgctga aaacgttgca
    26881 gatttccgta ctcctcatcg agattgacac cggaaatcga ctgctgctga ttggaaagct
    26941 gcgtcaccac attaccttgc gtggtgctac tggttttcaa cgtcgcggtt ttattaccga
    27001 tatcactcac taacgaggca taagcgtcgt taaaggattt cgcaccgccc accgttttac
    27061 tgttgctttg cagatcgagc agggcctgac cgttgcggtt gtcgctatca cctgtatctt
    27121 ctgcgctcgc catggcgatt ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg
    27181 cgtcacttac cggtttcagc gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca
    27241 actccagacc atcaaatgcc actttaccgc tggcatccgg cgtcaccgta aaagtggtat
    27301 tactggcaag gcgggtgacc tgccactgat tattatcgaa cgagattttg taatctgtcg
    27361 ccagtaccgc ggaggcatca gttaccgttg caccgatcgc aacgtcacct ttgtttttcg
    27421 tgttttgcag aaccgcgggc ttaccgatag caaagaaatc ttcaccggca tcgccgttag
    27481 catcaaatcc ggctttgtgt tgggtgttga aagcctcggc aaatgccagc gccagttgtc
    27541 caagcgtatt acgcgtctgg tccagatcct gagaacggaa tgtcagaatg ccgcccagcg
    27601 acccggtatt cagtaatttc tccgggatct caatattgcc tgccgtccca tcaacataag
    27661 cgacagtcgt acgagaaggg tcagcgctgg aaggaactgc cgccagttgc cgagccgtac
    27721 ttccctgaac cagtgagtaa ccattggcca tcgtgatgtt ataagtgcca ccatcctgaa
    27781 cgctgacttc tacaccaaca atctggttta attcgctcac cagttgatcg cgttgatcca
    27841 gcagattgtt aggtgacgcc cctgccccca cgcctgtcag gcgggagatt tgatcgttca
    27901 ggctggcgat ttgtttagcg taattgttga tctgatcaac gctggcacct atcgcgatat
    27961 tgacctgttt gtcctggtcg cgcagatatt gatcggtggt tttaaactga ttcaccaacc
    28021 cttctgattt cccaatcagc gcctggcgcg ctgccggatc ttccgcgtta ctcaccagcg
    28081 tttgcaggct ggtgaagaaa tcctgcatct gtgttgccag cgaagaggta ctggtggaga
    28141 gcatattgtc gattttcgac atctgctcat agcgggcggt caggccgcta ctttgcgtct
    28201 gcgcagcacg taactggttg gtaataaacg catcatactc acgctgcaca ccagaaacgt
    28261 agacgccatt gccaacccag ccgccagcgc ccaacgtgct attggcctgc gccataatag
    28321 tggtttggcg ggtatatccg gcaacgttat agctggagat attattactt gccgtattta
    28381 acgccgcctg ggccgcgttc agtccgctca tggcgttatt gatcaagctg gacatggagg
    28441 ttccttataa gccttcaatt acagagtatt atcggcagcg ccccgccgga cttgagttat
    28501 tcagaacaga ttatcaatgt tcatgctgta ggttttgctc accttgtcgc ttatcgattt
    28561 catctgctga atcatgctgg tgagtttgcg ggcatagtga ggatcggtgg catagcccgc
    28621 gtcctgtagg gcctgcgccc cctgctccgc actcgcggcg gtcgtcacgg cggcgtagcg
    28681 cgggttacgc gttaacagcc caacgtaatc cgacaatgct tccagatacg agctgtagac
    28741 ccgaaacttc gcttttactt tcttcgcttc gccattttca tattcagtcg tggtgatttc
    28801 agtgactggc cctttccagt tgccagaggc tttgacgcca aacaggttat agctcggctc
    28861 gccgttttca cggcggattt gccgttgtcc ccagccagat tccagcgccg cctgagcgag
    28921 gatcaaatga tgtggcacac cgctttgctg gcttgccagt tgggcgggca acgagagttg
    28981 cgcgaggaat gctttactgt cacccggcag cgaatcatcg tagttacgtg gtacggcctt
    29041 ttgcaccagc tgcgaaagcg tctgattttg ataacgcacc acggtttcga gcgggaattt
    29101 catcggtgct gctggcgtgg actcctctgg caatggttgt tctggcgtca tctgtttaac
    29161 catcatctct gccagcccca gacctttgcc cgccgtcatc tgttgggcaa tctgctggtc
    29221 atacatactg gtatacaggc gagtgtgctc gctgctgaac aggccatctt ttggtaaagc
    29281 gtcgcgcatg cttttcaaca tcatctgcac gaacatccct tccacctggc gggccaccgg
    29341 acggatattt gccgccggat cttcgcccgc tttcgcctta agttcgttga gcgattgtgc
    29401 atcccaggcc gcacttgcca gtagtttgct gtcgctgatc atcagatgat ttccagtttt
    29461 gcccgcagac atcccgcact ttgcattgat tgcaaaatag acatcagatc catcggcgta
    29521 gcgcccagcg cattgagcgc acgcaccacg ttattgaggc tggcgctgga acgtacgctt
    29581 tgcagcgaac cgccgctctg gcgtaaatcg atctgcgttt gtggagtaac cacagtctgt
    29641 ccaccaccaa acggtgtatc tggctggctg acattggcct ggcgattaac tgttactgag
    29701 agatttccct gcgctaccgc gcagctgtcg agggtcactt cgcgattcat caccaccgaa
    29761 ccggtgcgcg agttaatcac tactttagcg tcctgcgggg taacattaac ctgcatattc
    29821 tggatatcgg caaggaaacg gacctgggaa ctgttgccac tcggtacgcg cacctgaata
    29881 gtccgcgcat ccaacgcggt ggcgctgcca tatccacgca ctcggttgat ggtgtcagcg
    29941 atttgctgtg ccatgctgaa atcttcgtcg ttaagttgca aattaagggt attcccgacg
    30001 ccaaactggc tgggcaattc acgttcaata accgcaccat tggtgatccg tccaccgttc
    30061 agttggttca cctgaacact gctaccgcca gcggaggccc ctgcgccgcc aaccagaata
    30121 ttgccctgcg ccagcgcata cacctgactg tcaacgccct taagcggtgt catcaataac
    30181 gtaccgccac gcagactttt ggcatttccc atggaagaaa ccaccacgtc aatggtttgc
    30241 ccctgacgtc caaacggtgg aagtgacgct gtcaccataa ccgcagcgac gttttttagc
    30301 tgcatattag tgcccgtcgg aacggtaatt cccagctgtg agagcatgtt attaagcgtt
    30361 tgtgtggtaa acggcgtctg agttgtctgg tcaccagttc catccagccc caccaccagg
    30421 ccatagccaa tcagtgagtt ttgccttacc ccctgaacac tggtgagatc gcgaatacgc
    30481 tcagcctgag ccgccgtcgt aaccagtaga agaattaatg cagagagaaa tttaatcacc
    30541 acagcctcac ttacattgga gacaggttaa gaaagaaacg ctgcaaccag cccatatttt
    30601 gcgcttcgtt aatgtagcca ttgcctacgt attcaatgcg cgcatccgcc acctgagtag
    30661 acggtacggt attgctgccg ctgatagtgc gtggattaac cacgccagag aagcgaataa
    30721 attcggtacc ctgattaatg gcgatctgtt tttcacccac cacatgcagg ttgccgttaa
    30781 ccagtacctg gtcaaccgtc accgtcaacg tgccgctaaa ggtattactg gcattggccc
    30841 cgccttttcc gttgaacgtg ttaccaccgg aggcttcgac atcggcacga gcgttaccaa
    30901 acagcccctg caaatagcgc ggcacagtat caaagccaaa attagtttta ccgtcacggc
    30961 tggcattcgc agaggagctt ttgctggcgc tgacgttctc ctgcaacacg atggtcagcg
    31021 tatcgccaat attgcgtggt cgacgatctt caaacagcgg ttgatagcca tagttaatcg
    31081 gctgagcaga ctggaaaata gaaccgttgg cgacgggcgt cggaccggga accggttgtg
    31141 cactggtcgc cccctgtacc agcggcgtgg agggtatcca ggcgcagccg gttagtgaaa
    31201 gcaccaacaa gctggaaatg gcataagtat gcgcagcgtt tttttgcatg gctatcatct
    31261 tcaaaaatca gtaaaccggt gaatctgcca ccggttatgc cttagagttg cgtcagtttt
    31321 tgcagcatct gatcggtggt ggataccgct ttactgttga tttcgtaagc gcgttgcacc
    31381 tgaatcatat tgaccagttc ttccgccacg ttgacgttag acgtttcaac atacccttga
    31441 tacagcagtc ccgcgccgtt caggcccggc gtgctttcgt tcggtgcacc ggaggattgc
    31501 gtttcggtgt agaggttttc gccaatactc tccagtccgg tatcattcat aaaggtggtg
    31561 agattgagct gcccaacctg aaccggagct gcctggcctt gttgtgttac gctgaccacg
    31621 ccatcacgac cgatggtgat acttagcgca ttcgccggaa tggtgatcgc tggctgcacc
    31681 tgaaaaccac ctgccgtcac cagctgcccg ttctgatcca cctggaaaga gccgtcacgg
    31741 gtataggctg atgagccatc tggcaacatc acctggaaaa agccctgccc tttaatcgcg
    31801 acatctttgc tgttgttggt ctgcgacaga ttgccctggc tgtgtaagcg ttcagtggcg
    31861 accgggcgta cacccgtgcc gatttgtaat ccggatggta aggtggtttg ttcggaagac
    31921 tgagcacctg gctggcgaat ggtttgataa agcagatctt caaataccgc gcgctgacgc
    31981 ttaaaaccgt tggtactgac gtttgccagg ttgttggcaa tgacgtccat attggtttgc
    32041 tgggcatcca ggcccgtttt ggcgatccat aatgaactta tcataaaatg tcctgtagtg
    32101 gattagtgac aaagtgcatc tgtcttcttg ccggatgcgg cgtaaacgcc ttatccggcc
    32161 tacaagagct cgcgaaatca ataaattgcg attgccccat agacccgata aacttgcgca
    32221 tcgggcaact tatacttgtc atgtatcctt tcaattaact catcgacagc agttggttgg
    32281 ctcggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca aaacgccgcg
    32341 cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct tccagaacac
    32401 ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg cccacgcgtg gtctgggttt
    32461 ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctggac ttcgctgccc gtggctttca
    32521 ccagtttcag acgccccact ggcgcaaccg tatttgccgg atcgcccgga ttcagcgccg
    32581 agattgtgcc atcggcagca atagtgattt ccgccccttc cggcacagca attggcccag
    32641 cctcgcctat caccggatgt ccctgaattg tcagttgccc ggtgggatca acctgaatgc
    32701 tgccattacg cgtataccct tcgctaccgt cagcggtctg cacggccagc cagccatcct
    32761 gctgcaacgc aacgtccagc gggcgcgagg tgtaatccat cttgccgggc gtcatgtctg
    32821 cacccggcgt tgacgccgtt accagcgtgc gcgtaggcag tgaaagccct tccaccggca
    32881 ctgcgcgcaa ggcgtttaac tgcgcgcgaa aaccgggcgt tgaggcattg gccagattac
    32941 tggcggttac cgcctgttga ttcaacgtct ggctgcctgc tcccatcgcg gtataaattg
    33001 cgtgatccat tgagctatcc cgtcagcgat tagcgtaagt taaccagcgt gttgaggatc
    33061 tggtcctggg ttttgatggt ctgggcgtta gactgatagt tacgctgagc aacgatcata
    33121 ttgaccagtt ctttactgag atcgacgttg gacgcttcca gcgcaccgtt ggtcagggtg
    33181 ccaaagttgc ccgtcccggc tgtccccaac agcgccacgc cagaagattg cgtcgcagac
    33241 cagacgttgt cgccttcgga tgccagacct tcgttgttgg caaagttcgc cagtacaatc
    33301 tgccccagca gttgggtttg ttcgttggaa tagttgccga caaccgtacc gtcatcattg
    33361 atttgataac tcaccagatc gcccggtttg tagccgttct gggtggttgc cacaatgttg
    33421 ttagcgccgg tattttgctg catggagttg aggaagctca ggctaaacgt ggcgggttct
    33481 gcaccgttaa ttgcgccagt ggaaatattc gctgttggat ctgaggtcag aacgccattg
    33541 gcattaaaca ccagcgtcat tgcaggctca gctgtacctg ttggatcact gctatcctgg
    33601 gtgtagacct gccagttatt atccccggtc ttcacaaagt agacgctcat gtcatgagca
    33661 ttaccctgac tatcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgcta
    33721 gcatcaaatg cgttaacaga gggaagcgga tcactggaat tcaggttgat ctgcatcgac
    33781 gccgtggtgg tagttttcgc tgccatcagg gtattcggaa tcgaaatatt ggtcggattc
    33841 gccccttgct gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag ctgtaaacct
    33901 tgcatattca ccaggttacg gttttcatcc agcttaaatt gtccgttacg gctgtagaac
    33961 accgaaccgt tgctgtctac cagacggaaa aaaccgttct ggctgatagc aacgtccaga
    34021 cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc agcaactttt
    34081 acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta
    34141 aagccgtagg tggcagagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca
    34201 gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct
    34261 taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag atccagcgta
    34321 ttaccgttgt tgccgcggat cacaccctgc accagagcaa actgcagcgg ctgggcaacc
    34381 agttgtgtac caccgttact ggcgctaatc gctacgttgt aagaaccgtt cggcgcagtg
    34441 gtgccgtcag tcaacgtacc gtcccaggtg aaactgtgga ctccggcggt cagttcacca
    34501 atatcaatgg tacggacaac cgcaccgttt ttatcggtga tggtggccgt cactttgtct
    34561 gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg
    34621 ccggttcccg caagaacagt ggtgccgggg atcatcacgc cgtgaccgat caggttactg
    34681 gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt
    34741 ttttcaatcc cgctgaccgt gctgatttga gccaattgcg acgtcagctc gttgttttcc
    34801 attggattgg tcgggtcctg gtttttcagc tgcgccacca acaaagtcag aaaactgctt
    34861 tgtaaatctg cggcgttgct gcccgtgagc gaactactac tggtggtgct gacgccggta
    34921 tttgtcggat cggtggtggt taccgcaatg gacatagttt tctcctttat tgaccgagcg
    34981 taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgatagc
    35041 tgcgtgacgc cgacatggtg ttaaccatct ctccgacgac atcaacgttt ggcattttca
    35101 cgtagccctt tgcatccgcc agcggattac ccggttcata aaccagtttg tccggggcct
    35161 gactttctat aacatcggca acctttacgc cgcctgtcgc agcacctggc gcagcgttaa
    35221 cctgaaatac cacctgtttt gcccgatatg gctgtccatc gggaccggtc acgctatcag
    35281 cattcgccag attactggcc gccacgttca ggcgctggga ctgagcagtt aacgccgacc
    35341 cggcgatatc aaaaatattc agcagtgcca tccgttaatt tccgctctgt aaaacgttca
    35401 tcatgccttt gatttgcccg ctcaacgcgc taaggctcat ctggtattgc aggctgttat
    35461 cggcaaactg ggtgcgttcg cgatccatat cgacagtatt accatccagc gagggctggt
    35521 ccggaatacg gtattgcagt tctgcggtag gaggcgtcag cgcctgcgcc ggaatgtgtt
    35581 gcgttgaggt catcgtcagt gcaaccacgc ttgttgcatc ccgtccacgt tgcatgactt
    35641 ttttaagttc actggcaaaa tcgatatcgc gcgcctgata accaggggta tcggcattgg
    35701 cgatgtttgc tgccagcact tcctgacgct gggcgcgcag attgagcgcc tcttgttgaa
    35761 aacgtaaggc ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca aaattcggcc
    35821 atcagcttaa atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa tgggtcgcta
    35881 tttatgccgt tgatggtcat tgcggacagg tacaattcac gttgtagaaa tggctggggg
    35941 gcgaaaatgc tggcaataaa acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta
    36001 agtgcggcga gcaatctcac atcgcaattg cacacctttt ttagcgccca gctcgcgggg
    36061 gtaagtgatg aggttcgtgt ttctattcgt acagcgccca atctactacc gccgtgcgag
    36121 cagccattgc tttcgatgag caataattcc cgcctgtggg gcaatgtgaa tgtgttggca
    36181 cgctgcggta acgacaaacg atatttacag gttaatgtgc aggccacagg aaattatgtg
    36241 gttgccgcaa tgcccattgt gcggggagga aagctggaag ctggcaatgt caaactgaaa
    36301 cgcggacggc tggacacctt gccaccgcgt acggtgctgg atatcaatca acttgttgat
    36361 gccgttagcc tgcgcgatct atcacccgat caacctatcc agttaaccca gtttcgccag
    36421 gcatggcggg taaaagcggg acaacgcgtc aacgtgatcg ccagcggtga tgggtttagc
    36481 gccaacgcag aaggtcaggc gctgaacaat gctgccgtcg cacagaatgc gcgggtgcgc
    36541 atggtatcgg gacaagtggt cagcggcgtt gttgatgcag atgggaatat tcttataaac
    36601 ctgtaagctg ttaaagatta cccgtccctt gccgataaat aagcaacaca tgataaaagc
    36661 gccctcaatg aggaataaac catgagtatt gatcgcactt cgcctctgaa gcccgtaagc
    36721 accgttcaac cgcgcgaaac cactgacgcg ccggtaacga acacccgggc ggcaaaaaca
    36781 accgcctcca ccagcaccag tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc
    36841 ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa aactagcgat tcgtaacggt
    36901 gaactaaaaa tggacaccgg caaaattgcc gatgcgctga tcaacgaagc gcagcaagac
    36961 ttgcagagta actgatcgta tgacgcgtct tgcagagatc ctcgaccaga tgtccgctgt
    37021 gcttaacgat ctcaaaacgg taatggatca agagcagcaa catctctcta tggggcagat
    37081 caacggcagc cagttgcaat ggattacaga acaaaaaagc tctctgctgg cgacgctgga
    37141 ttacctcgaa cagttacgca ggaaagaacc caatgcagca aatagcgttg atattagtca
    37201 acgctggcag gaaattactg tgaaaacgca gcaactacgc caaatgaatc aacataacgg
    37261 ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca
    37321 tcaggaaccg acgctatatg gagcgaacgg tcagacctca acaacccatc gcggcagtaa
    37381 aaagatttcg atctgaagat tatctccggc ctgcactgca ggccggaatg cctttttaca
    37441 ccgtccggcg ggcaaactct ttaactttga agcccagtac cgccagtgct gcaaagtacg
    37501 cggcaatccc cgccagcacg accgccatta aacgcagtaa acgccagggc atggtaccca
    37561 atgaccactc cggcatgata tgtaacatac ctaaaagcac gccagacatc accagtaccg
    37621 ccaccaccag acgcaacaga aacgccatcc agccgggttg cggggtaaag attttctgct
    37681 tacgcaactg ccagtaaagc agcgaagcat tcagacacgc cgccagacca atagaaagtg
    37741 acagcccggc atgtttcaac ggaccgataa acgccaggtt catcaattgc gttaaaatca
    37801 gcgtaacgat ggcaattttc actggcgttt taatgtcctg gcgggaataa aagccaggag
    37861 ccaacacttt cactacaatc aggccaatca aacccaccga gtaggcaatt aacgcccgct
    37921 gggtcatcag cgcatcaaag gcggtaaatt taccgtactg gaacagcgaa acggttaacg
    37981 gacctgaaag aatgcccaac gcaaccgcac tcggcagcgc caacaggaaa caaagacgca
    38041 gcccccagtc catcaaacgg ttgtattcat catgattgcc actggcaaaa cttttcgaca
    38101 gcgacggcag caaaatggtg ccaagcgcca cgcccagtac accggacgga aactccatta
    38161 agcggtcggc gtaatacatc caagacaccg aaccggaagc aagaaacgag gcaaaaatgg
    38221 tgttgatgat taaggagatc tggctcacag agacgccaag gatcgccggt cccatctgtt
    38281 tcaccacgcg cattgctccg gcatcgtgga agttaatgcg cggcaggacc agcatgccga
    38341 tcttcttcag gtgcggtagc tgatacacca gctgcaggat gccgccgacc gtaacagccc
    38401 acgccagcgc cagcaccggt gggttaaagt acggtgcggc aaacagcgcg aaaccaatca
    38461 tgctgatatt aagcagtgtt ggagcaaacg ccggaatcga gaagcggttc cacgtattca
    38521 gaatcgctcc caccagcgac gccagggaga tcagcaagat atagggaaag gttatcttta
    38581 gtagctggct ggtcagggca aatttgtcgg cagtgtcagc gaagcctggc gcggtcacca
    38641 tgatcaccca cggtgcagcg agcatgccag cgaccgtcac aaccgccagc gcgagtgtca
    38701 gcaggccaga aacataagag acaaagaccc gcgtggcgtc ttcaccctgc ttgcttttat
    38761 attctgccag aatcggtaca aatgcctggg aaaatgcccc ttcggcaaag atacggcgca
    38821 acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga
    38881 ttctggcgac aattgcgtca cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc
    38941 tgacggcggc cagcgatttt aataaattca tcggtgttct aatccagacc cacgggcaaa
    39001 acgcctgcaa agcaggcgct gaaagaggcg aatagtctac ctggattatg gtgaattgct
    39061 accgccagat gttacagggt ttattcactc atcgcatcgc gccagatctt gtcaacgata
    39121 cgttgcgccc gcactgcctg ttcgccggcg gtttgcggaa ctgtctgatt ttgcacgcat
    39181 tcaatgaagt gccgcgcaca gccgacaaat ccacgttgct caagcgtact ctgccaacca
    39241 ggaatcggtt tatgcactac gccctgcccg cgctcctcac gccattcgcg catatccgta
    39301 atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt cacgctggct tccggcacgg
    39361 cgatgcatac aggtggtgat ctgcaaagga ccggccgaaa aatggtgctc ggcaaacagc
    39421 atttcgccag cgtcgttagt cagtagcgta ccgccatcca gagaggcttt gccgcccgac
    39481 aaccacagcg cggtatccac cacatgcaga taatcatcca gcaacgtgaa ataaagatcg
    39541 tgtggcccga cgctattgct acgatgttta tccattctta gcgaggctgc ggtggcgagt
    39601 tgcgttttta actctccgta gagtggtgcg aaacgacggt taaaaccgac catcaacgtc
    39661 agttttttcc gcgccgccag ttccaccaac cgttcagcat cgcgcagatt ttctgccagc
    39721 ggtttatcga cacagacatg tacccccgca ttgagtaacg tactgaccac gtcaaagtgg
    39781 ctggcggtgc tggaatgcac aaaaaccgca tcgcaactgg cggcaaggct ggataacgaa
    39841 tcggcataag gaatgcgcca gctttcacaa attggcaggg ctttcgcgcg cgtaggcgac
    39901 caggctcctt gtaacatcca gtcagacgct gccgccagca ccggtaacca cgctttttgc
    39961 gcaatgccgc ctaatcccac tacgccgata cgtaattttt tcactttaat ctcccagatg
    40021 ggcaagcaac gaatcaagac gctgtttcag ttctgccact tcgatttcca gggcttcgac
    40081 gcgagcctgt aaatcaccgt caaccgcgtt cgacatatcc atcaccgccg gctgatcttc
    40141 aacctcacca ctgaaaagat gcatgtagcg gctttcacgt ttacccggtt cgcgggccag
    40201 acgcaccaca aaaggaccat cttcgcgatt tgccagttgt tccagcgtcg actccacttc
    40261 cgccatatcg ctgaattcat acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg
    40321 ggcaccacgc aataacaacg tggtgattaa cgccacttct gcggcgctca gtttcagatc
    40381 gccaaattct gaattacaaa aacgttgctc atatttggtg acccgattac caaaaccgct
    40441 cactgtgcgt agataatgac gtttgaccag attatccagc tgttcctgca cttcggattc
    40501 actcagattc atgaccggtt cacggttcgt tttctgatta caggccgtga ctacaccatt
    40561 gactgagagc gggtattgct ccggcgtggt cacctgcttt tccagcaagc aaccaatcac
    40621 gcgagcttca agggcagtaa gttgatattt catgagattc tccttagcgg ccgggcgtcc
    40681 agtctggggt agttaatgcc gtcagtacgt gatcgcgcca ttgtccatca atcaacagat
    40741 agtctttcgc atagccttct ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt
    40801 tattgtgcgg catataatta gccataatgc gatgaatatg ttgggtgcgc tgcatataac
    40861 gaatggctgc ggtcagggct tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg
    40921 aataaccgag atagcaggca tgaaaagagc cacgaacaac attggaaaaa ttggcaacgc
    40981 caataatctc tttttcgtcc gggtcgaata agccaaagta gaaagctgaa ccttgtttat
    41041 gaaattcgtt aatcatccct agcctggcct gccagcctga tggataacag tggctttcgt
    41101 cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc tgcgtaataa tccgccagac
    41161 gccaggcatc acgatcatgc accagacgca cgaccagtcg gtctgtggtt aagcgcactt
    41221 ttggcacgtt actgcgatag ccaaacattc tatacctact ccttcccgta acgtctttaa
    41281 tacgctgtaa gtattactat acctgcgcaa ccagaccctg tgaaaagtcc agaatgccat
    41341 tttctgcatt attcacatat ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa
    41401 aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca
    41461 tcttttattt cccgggaggg gaaatgtccc gcgtgtcgca ggccaggaac ctgggtaaat
    41521 atttcctgct catcgataat atgctggtcg tgctggggtt ctttgttgtc ttcccgctga
    41581 tctctatccg cttcgttgat caaatgggct gggccgccgt catggtcggt attgccctcg
    41641 gcctacgcca gtttattcag caaggtctgg gtattttcgg cggagcaatt gccgaccgct
    41701 ttggtgccaa accgatgatt gttaccggta tgctgatgcg tgccgccgga ttcgccacaa
    41761 tgggtatcgc ccacgaaccg tggctactgt ggttttcatg cctgctttcg ggactcggcg
    41821 gcacgttgtt tgatcctccg cgttcggcgc tggtggtgaa attaatccgt ccacagcagc
    41881 gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc cggtgcggtc attggcgcat
    41941 tgttggggag ctggctgttg caatacgact ttcgcctggt ctgcgccaca ggggcagttt
    42001 tgtttgtgct gtgtgcagcg tttaatgcgt ggttgttacc ggcatggaaa ctctccaccg
    42061 tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg cgacaagcgt tttgtcacct
    42121 atgtactgac gctggcgggt tactacatgc tggctgtaca agtgatgctg atgctgccaa
    42181 ttatggtcaa cgacgtggcg ggcgcgccct ctgccgttaa atggatgtat gccattgaag
    42241 cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg gagtgaaaag cattttcgtc
    42301 tggaacaccg gttgatggct gggctattga taatgtcatt aagcatgatg ccggtgggca
    42361 tggtcagcgg tctgcaacaa cttttcaccc tgatttgtct gttttatatc gggtcaatca
    42421 ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc ggacgcaaga gctcgcggca
    42481 gctatatggg gtttagccgt ctgggtctgg cgattggcgg cgctattggt tacatcggtg
    42541 gcggctggct gtttgacctg ggcaaatcgg cgcaccagcc agagcttccg tggatgatgc
    42601 tgggcattat tggcatcttc actttccttg cgctgggttg gcagtttagc cagaaacgcg
    42661 ccgcgcgtcg tttgctggaa cgcgacgcct gatttgatac tcccccctga ctcctccata
    42721 ctgaaagtga actgacagcc aaatggagga gtcacgtgaa gctatacatt tacgatcact
    42781 gcccttactg cctcaaagcc cgcatgattt tcggcctgaa aaatatcccc atcgaattac
    42841 atgttctgct caacgacgac gcagaaacac ccacccggat ggtcggtcaa aaacaggttc
    42901 ccattctgca aaaagatgac agccgctata tgccagaaag catggacatc gttcactatg
    42961 tcgataaact cgacggcaaa ccgttactga ccggcaaacg ttcccctgcc attgaagagt
    43021 ggctgcgcaa ggtcaatggc tacgccaaca aactgctgtt gccgcgtttt gccaaatcgg
    43081 catttgatga gttttctact cctgccgcgc gcaaatattt cgtcgacaag aaagaggcta
    43141 gcgcgggtaa ttttgccgac ctgctggccc actctgacgg tctgattaag aatatcagcg
    43201 atgatttacg tgcgctggac aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt
    43261 cggaagatga tattcagcta tttccgctcc tgcgtaatct gacgctggta gccggaatca
    43321 actggccaag ccgcgttgct gattaccgcg ataatatggc gaaacagaca caaatcaatt
    43381 tgttatcatc aatggcgatt taatcctgac ccgccgcggc agcctgtatt cgcgggttgc
    43441 cgccgttttc agttttttct gctggcgcgt ttatcgctgg tgacacatgc tgcgttgtgt
    43501 caggatattg acgcagattt catgtcgagg actaccatga acaagttttt atttgccgct
    43561 gcattgatcg tcagtggcct gctcgttggc tgtaatcaac tcacccaata caccatcacc
    43621 gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt tctcaaaaga tatcggttta
    43681 cccggcgtgg ctgacgccca tattgttctg acaaacctga ccagccaaat tggtcgcgaa
    43741 gagccgaata aggttaccct aaccggagac gccaatctgg acatgaactc cctgttcggt
    43801 agccagaaag cgaccatgaa actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa
    43861 ggcgctatct tcctgaaaga gatggaagtg gtcgatgcga cggtacaacc ggaaaaaatg
    43921 caaacggtga tgcaaacgtt gcttccctat ttaaaccagg cattacgcaa ttactttaac
    43981 cagcaacctg cttacgtcct gcgcgaagat ggcagccagg gcgaagcaat ggcgaagaaa
    44041 ctggcgaaag gcattgaagt gaagccaggc gaaattgtca ttccgtttac tgattaatca
    44101 cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa aacgtttccg cttatccttt
    44161 gtgtccggca aaaacatccc ttcagccgga gcatagagat taatgactgc accatcccag
    44221 gtattaaaga tccgccgccc agacgactgg caccttcacc tccgcgatgg cgacatgtta
    44281 aaaactgtcg tgccgtatac cagcgaaatt tatggacggg ctatcgtaat gcccaatctg
    44341 gctccgcccg tgaccaccgt tgaggctgcc gtggcttatc gccagcgtat tcttgacgcc
    44401 gtacctgccg ggcacgattt caccccgctg atgacctgtt atttgacaga ttcactagac
    44461 cctaatgagc ttgagcgtgg atttaacgaa ggcgtgttca ccgctgccaa actttacccg
    44521 gcaaacgcaa ccactaactc cagtcacggc gtgacgtcag ttgacgcaat catgccggta
    44581 cttgagcgca tggaaaaaat cggtatgccg ttactggtgc atggtgaagt gacacatgca
    44641 gatatcgaca tttttgatcg tgaagcgcgc tttattgaaa gcgtgatgga accgctacgc
    44701 cagcgtctga ctgcgctgaa agtcgttttt gagcacatca ccaccaaaga cgctgccgac
    44761 tatgtccgtg acggaaatga acggctggct gccaccatca ctccgcagca tctgatgttt
    44821 aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc tgtattgtct acccatcctc
    44881 aaacgcaata tccaccaaca ggcattgcgt gaactggtcg ccagcggttt taatcgcgta
    44941 ttcctcggta cggattctgc gccgcatgca cgtcatcgca aagagagcag ctgcggctgc
    45001 gcgggctgct tcaacgcccc aaccgcgctg ggcagttacg ctaccgtctt tgaagagatg
    45061 aatgctctgc agcactttga agcattctgt tctgtaaacg gcccacagtt ctatggcttg
    45121 ccggtcaacg acacattcat cgaactggtc cgcaaagagc aacaggttgc tgaaagcatc
    45181 gcattgactg atgacacgct ggtgccattc ctcgccgggg aaacggtacg ctggtccgtt
    45241 aaacaataaa aatcgtagct tcctgttgtc attaggttat tttaactgta taaataacca
    45301 gtatattcaa cagggggcta ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt
    45361 gccagctggg gctattgacg ccctggctgg cgaactttcc cgccgtattc agtatgcgtt
    45421 tcctgataat gaaggccacg tatcggtacg ttatgccgca gcgaataatt tatcggttat
    45481 tggcgcgaca aaagaagata aacagcgcat tagcgaaatt ctccaggaaa cgtgggaaag
    45541 cgccgatgac tggtttgtca gcgaataata tgcagtgatt tttgtttttg ccgggtcgcc
    45601 ccggcttttt tattttactt gcgaattgtg agtacaaaat ttctacactt cctcaaaata
    45661 attgcacaat atgctaaaaa aatatgaatg acatgctgtt ttattgttca tataaatctt
    45721 aaaagcaaaa atttatctgt tccccccttt aaaaattcgg tgaataccct tacttattgg
    45781 tatactgaag ttgcttcaga taaaaacgca ttgaacctcg aataacgttg tctagtaaca
    45841 cgaattaggg ggccatgatg gaaaaaaata atgaagtcat tcagactcat ccgctcgtag
    45901 ggtgggacat cagcaccgtt gatagctatg atgcgctgat gttgcgtttg cactaccaga
    45961 ccccaaataa gtccgagcag gaagggactg aagttggtca gacgctctgg ttaaccactg
    46021 atgttgccag acaatttatt tcgatattag aagcaggaat cgccaaaatt gaatccggtg
    46081 atttcccggt aaacgagtat cggcgtcatt aacatgacat gaccatccac ccaaaaggca
    46141 cctcaccggt gcctttacca tttctgggtt gtatatctgc attcagttaa ttactctgct
    46201 taaaccatga gagcagagag aaaaaaatga aatacgatct catcattatt ggcagcggtt
    46261 ccgtaggcgc tgccgccggg tattatgcaa cccgtgccgg tttaaacgtg ctaatgaccg
    46321 acgcccatat gccaccgcat caacacggca gccaccacgg cgatacgcga ttaattcgcc
    46381 atgcttatgg tgaaggcgaa aagtatgtcc cgctggttct ccgcgcgcaa acgctgtggg
    46441 atgaactctc ccgccacaac gaagaagatc ccatttttgt acgctctggc gtcattaacc
    46501 ttggcccggc tgattccaca tttctcgcca acgtcgccca cagcgccgaa caatggcaac
    46561 tcaacgttga aaagctcgat gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc
    46621 cggacaacta catcggctta tttgagactg attccggctt tttacgcagc gaactggcga
    46681 ttaaaacctg gatccaactg gcgaaggaag cgggctgtgc gcagctgttc aactgcccgg
    46741 tcaccgcaat tcgtcatgac gatgatggcg taactattga aacggctgac ggtgagtatc
    46801 aggcgaaaaa agcgattgtc tgcgcgggaa catgggtaaa agacctgctc ccggagttgc
    46861 ctgtccagcc cgtacgcaaa gtatttgcct ggtatcaggc cgatgggcgc tatagcgtga
    46921 agaataaatt cccggcgttt accggtgaac ttcccaatgg cgatcaatat tatggttttc
    46981 cggcagaaaa cgacgcgttg aagattggca aacataacgg aggccaggtt atccattcag
    47041 cggatgaacg tgttccgttt gcggaagtgg tcagcgatgg ttcggaagcc ttcccgttct
    47101 tgcgcaatgt attgccgggt atcggttgct gcctgtacgg cgccgcctgc acctatgata
    47161 attcgcctga cgaagatttt attatcgata ccctacccgg ccacgataat acactgctca
    47221 ttaccggcct gagtgggcac ggttttaaat ttgcgtcagt tttaggggaa atagctgccg
    47281 attttgcgca agacaaaaaa agcgattttg atttgacgcc attcaggctt tcccgcttcc
    47341 aataatcatc attacggcct ccagaatgag gccgttttta caggagtatt taatgcgtcg
    47401 gctgttgcac tatctcatca ataatattcg cgagcatctg atgctttatc tttttctctg
    47461 gggattgttg gcaattatgg atctgatcta tgtgtattat ttttagtttt ccgcactgac
    47521 ataaactgac aaatatttaa tctccctttc atttagcgtg aaaaattgaa tgagattatt
    47581 cctgcttcaa cagca
//
LOCUS       DAZGHF010000020        45708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGHF010000020 DAZGHF010000000
VERSION     DAZGHF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.020"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(264..1664)
                     /gene="asnS"
                     /locus_tag="ACHK3A_001275"
     CDS             complement(264..1664)
                     /gene="asnS"
                     /locus_tag="ACHK3A_001275"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO8739388.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1833..3035)
                     /gene="pncB"
                     /locus_tag="ACHK3A_001276"
     CDS             complement(1833..3035)
                     /gene="pncB"
                     /locus_tag="ACHK3A_001276"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO8739389.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQYLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3301..5913
                     /gene="pepN"
                     /locus_tag="ACHK3A_001277"
     CDS             3301..5913
                     /gene="pepN"
                     /locus_tag="ACHK3A_001277"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO8739390.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVRWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQLLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(6119..6886)
                     /gene="ssuB"
                     /locus_tag="ACHK3A_001278"
     CDS             complement(6119..6886)
                     /gene="ssuB"
                     /locus_tag="ACHK3A_001278"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO8739391.1"
                     /translation="MNTARLNQGTPLLLNAVSKQYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(6883..7674)
                     /gene="ssuC"
                     /locus_tag="ACHK3A_001279"
     CDS             complement(6883..7674)
                     /gene="ssuC"
                     /locus_tag="ACHK3A_001279"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO8739392.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7686..8831)
                     /gene="ssuD"
                     /locus_tag="ACHK3A_001280"
     CDS             complement(7686..8831)
                     /gene="ssuD"
                     /locus_tag="ACHK3A_001280"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO8739393.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(8828..9787)
                     /gene="ssuA"
                     /locus_tag="ACHK3A_001281"
     CDS             complement(8828..9787)
                     /gene="ssuA"
                     /locus_tag="ACHK3A_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO8739394.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9780..10355)
                     /gene="ssuE"
                     /locus_tag="ACHK3A_001282"
     CDS             complement(9780..10355)
                     /gene="ssuE"
                     /locus_tag="ACHK3A_001282"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO8739395.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10592..11602
                     /gene="pyrD"
                     /locus_tag="ACHK3A_001283"
     CDS             10592..11602
                     /gene="pyrD"
                     /locus_tag="ACHK3A_001283"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO8739396.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            11776..12318
                     /gene="zapC"
                     /locus_tag="ACHK3A_001284"
     CDS             11776..12318
                     /gene="zapC"
                     /locus_tag="ACHK3A_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO8739397.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFNLEQAV"
     gene            complement(12315..13424)
                     /gene="ycbX"
                     /locus_tag="ACHK3A_001285"
     CDS             complement(12315..13424)
                     /gene="ycbX"
                     /locus_tag="ACHK3A_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO8739398.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEVLATAPAKIYGAAA
                     ADDTVNITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            13668..15776
                     /gene="rlmKL"
                     /locus_tag="ACHK3A_001286"
     CDS             13668..15776
                     /gene="rlmKL"
                     /locus_tag="ACHK3A_001286"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO8739399.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            15788..17695
                     /gene="uup"
                     /locus_tag="ACHK3A_001287"
     CDS             15788..17695
                     /gene="uup"
                     /locus_tag="ACHK3A_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8739400.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            17825..19078
                     /gene="pqiA"
                     /locus_tag="ACHK3A_001288"
     CDS             17825..19078
                     /gene="pqiA"
                     /locus_tag="ACHK3A_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO8739401.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADDPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            19083..20723
                     /gene="pqiB"
                     /locus_tag="ACHK3A_001289"
     CDS             19083..20723
                     /gene="pqiB"
                     /locus_tag="ACHK3A_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO8739402.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            20720..21283
                     /gene="pqiC"
                     /locus_tag="ACHK3A_001290"
     CDS             20720..21283
                     /gene="pqiC"
                     /locus_tag="ACHK3A_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO8739403.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            21539..21706
                     /gene="rmf"
                     /locus_tag="ACHK3A_001291"
     CDS             21539..21706
                     /gene="rmf"
                     /locus_tag="ACHK3A_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO8739404.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(21776..22294)
                     /gene="fabA"
                     /locus_tag="ACHK3A_001292"
     CDS             complement(21776..22294)
                     /gene="fabA"
                     /locus_tag="ACHK3A_001292"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO8739405.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(22363..24123)
                     /gene="ycbZ"
                     /locus_tag="ACHK3A_001293"
     CDS             complement(22363..24123)
                     /gene="ycbZ"
                     /locus_tag="ACHK3A_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8739406.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANIR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            24309..24761
                     /gene="matP"
                     /locus_tag="ACHK3A_001294"
     CDS             24309..24761
                     /gene="matP"
                     /locus_tag="ACHK3A_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO8739407.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(24837..25889)
                     /gene="ompA"
                     /locus_tag="ACHK3A_001295"
     CDS             complement(24837..25889)
                     /gene="ompA"
                     /locus_tag="ACHK3A_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO8739408.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVPGGASTKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(26245..26754)
                     /gene="sulA"
                     /locus_tag="ACHK3A_001296"
     CDS             complement(26245..26754)
                     /gene="sulA"
                     /locus_tag="ACHK3A_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO8739409.1"
                     /translation="MYTSGYAHRASSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            26972..27601
                     /gene="sxy"
                     /locus_tag="ACHK3A_001297"
     CDS             26972..27601
                     /gene="sxy"
                     /locus_tag="ACHK3A_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO8739410.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(27564..29726)
                     /gene="yccS"
                     /locus_tag="ACHK3A_001298"
     CDS             complement(27564..29726)
                     /gene="yccS"
                     /locus_tag="ACHK3A_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO8739411.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(29736..30182)
                     /gene="yccF"
                     /locus_tag="ACHK3A_001299"
     CDS             complement(29736..30182)
                     /gene="yccF"
                     /locus_tag="ACHK3A_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO8739412.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            30305..32359
                     /gene="helD"
                     /locus_tag="ACHK3A_001300"
     CDS             30305..32359
                     /gene="helD"
                     /locus_tag="ACHK3A_001300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO8739413.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRFYHHLDAHWQRWSSEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEVIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPSVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(32391..32849)
                     /gene="mgsA"
                     /locus_tag="ACHK3A_001301"
     CDS             complement(32391..32849)
                     /gene="mgsA"
                     /locus_tag="ACHK3A_001301"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO8739414.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(32945..33607)
                     /gene="yccT"
                     /locus_tag="ACHK3A_001302"
     CDS             complement(32945..33607)
                     /gene="yccT"
                     /locus_tag="ACHK3A_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO8739415.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            33780..34193
                     /gene="yccU"
                     /locus_tag="ACHK3A_001303"
     CDS             33780..34193
                     /gene="yccU"
                     /locus_tag="ACHK3A_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO8739416.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(34238..34555)
                     /gene="hspQ"
                     /locus_tag="ACHK3A_001304"
     CDS             complement(34238..34555)
                     /gene="hspQ"
                     /locus_tag="ACHK3A_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO8739417.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(34613..35803)
                     /gene="rlmI"
                     /locus_tag="ACHK3A_001305"
     CDS             complement(34613..35803)
                     /gene="rlmI"
                     /locus_tag="ACHK3A_001305"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO8739418.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVNIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            35898..36176
                     /gene="yccX"
                     /locus_tag="ACHK3A_001306"
     CDS             35898..36176
                     /gene="yccX"
                     /locus_tag="ACHK3A_001306"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO8739419.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIH"
     gene            complement(36173..36502)
                     /gene="tusE"
                     /locus_tag="ACHK3A_001307"
     CDS             complement(36173..36502)
                     /gene="tusE"
                     /locus_tag="ACHK3A_001307"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO8739420.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRNFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(36593..37252)
                     /gene="yccA"
                     /locus_tag="ACHK3A_001308"
     CDS             complement(36593..37252)
                     /gene="yccA"
                     /locus_tag="ACHK3A_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO8739421.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(37662..38681)
                     /locus_tag="ACHK3A_001309"
     CDS             complement(37662..38681)
                     /locus_tag="ACHK3A_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8739422.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRTIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRSA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(38659..38901)
                     /locus_tag="ACHK3A_001310"
     CDS             complement(38659..38901)
                     /locus_tag="ACHK3A_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO8739423.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(38969..41407)
                     /locus_tag="ACHK3A_001311"
     CDS             complement(38969..41407)
                     /locus_tag="ACHK3A_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO8739424.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRFGIKGGFYWSTEKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQEKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPDLVHTLDTLD
                     VEAAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINDGLA
                     AECKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTAEEV
                     NKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETCDSVNQNQLETEQND
                     QKAEQNSQNALQDEPETKQSEPVAQQEEEKVCTACGQSGGGNCPDCGAVMGDATYQET
                     FDEKNQAEVQEDDPEEIEGAEHPNNENAGNDQHHTSDSETGEAADPLIAVNGHHEITS
                     TSRTCDHLMIDLETMGKNPNAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRD
                     TIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILR
                     RSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYV
                     SAIWQKLIPSQADF"
     gene            complement(41500..41688)
                     /locus_tag="ACHK3A_001312"
     CDS             complement(41500..41688)
                     /locus_tag="ACHK3A_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO8739425.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            complement(41685..41873)
                     /gene="dicB"
                     /locus_tag="ACHK3A_001313"
     CDS             complement(41685..41873)
                     /gene="dicB"
                     /locus_tag="ACHK3A_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO8739426.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            complement(42359..42511)
                     /locus_tag="ACHK3A_001314"
     CDS             complement(42359..42511)
                     /locus_tag="ACHK3A_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO8739427.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            42786..43088
                     /locus_tag="ACHK3A_001315"
     CDS             42786..43088
                     /locus_tag="ACHK3A_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO8739428.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNVKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            43091..43450
                     /locus_tag="ACHK3A_001316"
     CDS             43091..43450
                     /locus_tag="ACHK3A_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8739429.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(43497..43889)
                     /locus_tag="ACHK3A_001317"
     CDS             complement(43497..43889)
                     /locus_tag="ACHK3A_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8739430.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            44016..44276
                     /locus_tag="ACHK3A_001318"
     CDS             44016..44276
                     /locus_tag="ACHK3A_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8739431.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            44273..44710
                     /locus_tag="ACHK3A_001319"
     CDS             44273..44710
                     /locus_tag="ACHK3A_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO8739432.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            44797..>45708
                     /locus_tag="ACHK3A_001320"
     CDS             44797..>45708
                     /locus_tag="ACHK3A_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO8739433.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEVYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSELHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAIQEIRAAREQWERDNGFISNGNGLEA"
BASE COUNT        11554 a        11240 c        11735 g        11179 t
ORIGIN      
        1 ctttatcgac ctggcacaaa atcctctttc agacatccag aatgccaaaa aatagtatga
       61 aaattgtgct atcagctaag aaaaaactaa tccgcaataa gaatgcgggt tattttttgt
      121 gcgtaaatgt tgcaatgtaa tattccgtaa caggatgatc gttatggcgg ggttgcaaat
      181 agattgcttg acttaacaaa cagataactt gacagaaagg ataaaaaaag agccagcggg
      241 tgctggctct tgaagacgct aaattagaag ctggcgttac gcggagtacg tgggaacgga
      301 atcacatcac gcacgttttg cacaccagtt acgtaagcaa tcaggcgttc aaaaccaaga
      361 ccgaaacctg aatgcggaac agtaccgtag cgacgcagat cgcgatacca ccagtaatct
      421 tctttattca ggcccatttc cagcatacgc tcgtccagca cgtccagacg ttcttcacgc
      481 tgggagccac caatgatctc accgatgccc ggagccagaa cgtccatagc cgcaacggtt
      541 ttaccgtctt cgttaaggcg catatagaac gctttaatat ctttcggata gtttttaacc
      601 actaccggtg ctttaaagtg ttcttccgcc agataacgct catgctcaga agagagatcg
      661 acgccccagt aaaccgggtt ttcaaacttc ctgccgcagt tttcgagaat ggtcactgcg
      721 tcggtgtaat ccacctgcgc gaaatcggcc tcaatgaagc gttccagacg tgaaacggca
      781 tctttatcta cgcgttcagc gaagaatttc atgtcgtcag cgcgttcttc gagaaccgct
      841 ttgaagacat atttcagcat ggcttcagcc agacccgcga tatcgttcag gttagcaaac
      901 gccacttccg gctccagcat ccagaattcc gccaggtgac ggctggtgtt ggagttttca
      961 gcacggaaag tcgggccgaa ggtataaatt ttggacaatg cgcaagcgta ggtttcgccg
     1021 ttcaactggc cagatacggt caggaaagac tctttaccaa agaagtcttt gtcgaaatcc
     1081 actttgccct gatcgttacg cggcagattt tccagatcca gcgtagaaac acggaacatt
     1141 tcgcctgcac cttcggtatc agatgcggta atcagtggcg ttgaaaccca gaaaaatccc
     1201 tgctcgttaa agaagcgatg cagcgcctgc gccagcgtat ggcgaacgcg cgcgacggca
     1261 ccaatcaggt ttgtgcgcgg acgcaggtga gcgacttcac gcagatactc aatgctgtgg
     1321 cgttttgccg ccatcgggta agtgtctgga tcttcaaccc aaccagcaac ttcaaccttg
     1381 ctggcctgaa tttcaaattg ttgcccctgg cccggcgacg ccacgacttt acccgtcaca
     1441 atgaccgagc agccggtggt cagacgcaag acgtcttcat tgtaattggg cagagaatta
     1501 ttgatgacag cctgtacagg atcaaagcag gaaccgtcat aaacggcgag gaaggagatg
     1561 ccagcttttg aatctcggcg ggtacgtacc catccgcgca cggtgacttc gctgtcaacg
     1621 gctacgcggc cctggagtac gtcggctaca ggcacaacgc tcataatatt ctctctgtta
     1681 atagtcggaa aaaataaaca cttgtccacc cgtaaatggg gggataccta tgttacctgg
     1741 catctgcaat cagacaagca gaattcgcaa atgcagcgaa agatttcgga attaaaggta
     1801 aaaaaaggga gccgattagc tccctgatga tattaactgg cttttttaat atgcggaagg
     1861 tcgaacgctt tgcgtagcgc ccgaacaaac gctttatcgt ggcagatagt tttgcctggg
     1921 ctgtcagaaa gtttcgccac tggtttaccg ttacactcta ccaacttaat gacaatattc
     1981 aggggtttta cctgggggat atcgcaggtc aggcgagtcc caataccaaa acttaattgc
     2041 acgcgggaag agaagtggcg gtatagctca accgctttgc gtaaatccag attgtcagag
     2101 aaaaccagcg ttttactctg tggatcaatt cccagctttt cataatgtgc aatggctttt
     2161 tcaccccatt caaccgggtc accagagtca tggcgcaggc cctgatagcg actggcgaac
     2221 tcgacaccga aatctcgcag gaaagcatcc atagtgatgc agtcggttaa cgcaatgcca
     2281 agttggtcgg gatactcttc cagccaggca gcaagtgcag ctcgctggct gttggctaga
     2341 tccgggctga tttgctgatg cgcctggaac cattcgtgtg cctgtgttcc catcggcgtg
     2401 agggaaagcc gacgcgccag atcgtagttg ctggtgccca cgaaccagga ttcctgttgc
     2461 agacgtttca cgatggtttc ttgtacttca cgagaaaaac gtcggcgggt gccaaaatcc
     2521 atcagatgga agcgcgacat atcaagaccg gcggttaatg ccgagaagtc ggctaatttg
     2581 ctttccagcg tgtcgagggc ttgcgcaacg tcggcctgcg gtgagcgata gcgatgtacc
     2641 atttcactga taaccgccag caaaggaact tcccagagga tgacttcacg ccacgggccg
     2701 cttaaacgaa tatccagctt gccattatcg ttggacacgg tgacttgttc cgggttaaag
     2761 cggaactcgc gtagccagtt aagatagtcg gccttaaaga aaggcagggc agagagccac
     2821 tgatattcat catcctgcag gcgcaggtac tgcatcgcct gaacctgttc acgaatagca
     2881 tcggcataaa tacccagcag atcgtcacct cggcaacgaa actccgccgc gacatgcaca
     2941 tcgtaatagt gatgaaacac ggcttgctgc atatgcaact tataagcatc tgtatccagc
     3001 aacgagtgca gaacaggaga agcgaattgt gtcataggtg cgcagtagcg tcctcgtaca
     3061 ggagcgttta gtacaataaa catcttcagg aactgctgga gtataccttg tttcgcaatt
     3121 tattgaaccc cgatcacacc atatgccacc tttctggtcg atggcattac gcggtgtatg
     3181 ttataaaaat gtagcaataa aggcgtttgt acctgaaaag atgaagattc tgcatagcgc
     3241 gatttacgca acaggaatag actgaacacc agactctata aaagatgcta aaggttattt
     3301 atgactcaac agccacaagc caaataccgt cacgattatc gtgcgccgga ttaccagatt
     3361 actgatattg acttgacctt tgacctggac gcgcaaaaga cggtcgttac cgcggtcagc
     3421 caggctgtcc gtcatggtgc atcagatgtt ccgcttcgtc tcaacggcga agacctcaaa
     3481 ctggtttctg tttatattaa tgatgagccg tggaccgcct ggaaagaaga agagggcgca
     3541 ctggtcatca gtaatttgcc ggagcgtttt acgcttaaga tcgttaatga aattagcccg
     3601 gcggcgaata ctgcgctgga agggctttat cagtcaggcg atgcgctttg cacccagtgt
     3661 gaagccgaag gtttccgcca tattacgtat tatctcgacc gcccggatgt gctggcgcgt
     3721 tttaccacca aaattattgc cgataaaacc aaatatccct tcctgctttc caacggtaac
     3781 cgcgttgcgc aaggtgaact ggaaaatgga cgtcattggg tacggtggca ggacccgttc
     3841 ccgaaaccgt gctacctgtt tgcgctggtg gcaggcgact ttgatgtgct gcgcgacact
     3901 ttcaccacgc gttctggtcg cgaagtagcg ctggagctgt atgtcgatcg tggcaacctt
     3961 gatcgtgcgc cgtgggcaat gacctcgctg aaaaactcca tgaaatggga tgaagaacgc
     4021 ttcggcctgg agtatgacct cgacatctat atgatcgtcg cggtggattt cttcaatatg
     4081 ggcgcaatgg agaataaggg gctgaatatc tttaactcca aatatgtgct ggcccgcacc
     4141 gacaccgcca ccgacaaaga ttacctcgat attgaacgcg ttatcggcca tgaatatttc
     4201 cataactgga ccggcaaccg tgtcacctgt cgcgactggt tccagctcag tctgaaagaa
     4261 ggtttaaccg tcttccgcga tcaggaattc agctctgacc ttggttcccg cgcggtaaac
     4321 cgtatcaata atgtgcgcac catgcgcgga ttgcagtttg cagaagacgc cagcccgatg
     4381 gcgcacccaa ttcgcccgga tatggtcatt gaaatgaaca acttctacac cctgaccgtt
     4441 tacgagaagg gcgcggaagt gattcgtatg atccacactc tgcttggcga agaaaacttc
     4501 cagaaaggga tgcagctcta tttcgagcgt catgatggta gcgcagcgac ctgtgacgac
     4561 tttgtgcagg cgatggaaga tgcgtcgaat gtcgatcttt cccatttccg ccgttggtac
     4621 agccaatccg gcacgccggt tgtgactgtc aaagacgact acaatccgga aactgagcag
     4681 tacaccctga ccatcagcca gcgcacgcca gctacgccgg atcaggcaga aaaacagccg
     4741 ctgcatattc cgtttgccat cgaactgtat gataacgaag gcaaagtgat cccgttgcag
     4801 aaaggcggtc atccggtgaa ttcggtgctg aacgtcaccc aggctgaaca gacctttgtt
     4861 tttgataatg tctacttcca gccggtgcct gcgctgctgt gcgaattctc tgcgccagtg
     4921 aaactggaat ataagtggag cgatcagcaa ctgaccttcc tgatgcgtca tgcgcgtaat
     4981 gatttctccc gttgggatgc ggcgcaaagc ctgctggcaa cctacatcaa gctaaacgtc
     5041 gcccgtcatc agcaagggca gctgctgtct ctgccggtgc atgtggctga cgctttccgc
     5101 gcggtgctgc tcgatgagaa gattgatcca gcgctggcgg cagaaatcct gacgctgcct
     5161 tctgtcaatg aaatggctga actgttcgat atcatcgacc cgattgctat tgccgaagta
     5221 cgcgaagcac tcactcgtac tctggcgact gaactggcgg atgagctact ggctatttac
     5281 aacgcgaatt accagagcga gtaccgtgtt gagcatgaag atattgcaaa acgcactctg
     5341 cgtaatgcct gcctgcgctt ccttgctttt ggtgaaacgc atctggctga cgtgctggtg
     5401 agcaagcaat accatgaagc aaacaatatg accgatgcgc tggcagcgct ttctgcggcg
     5461 gttgctgcac agctgccttg ccgtgacgcg ctgatgcagg agtacgacga caagtggcat
     5521 caggatggtc tggtgatgga taaatggttt atcctgcaag ccaccagccc ggcggcgaat
     5581 gtgctggaga cggtgcgtgg cctgttgcag catcgctcat ttaccatgag caacccgaac
     5641 cgtattcgtt cgttgattgg tgcgtttgcg ggcagcaacc cggcagcgtt ccatgccgaa
     5701 gatggcagcg gttaccagtt cctggtggaa atgcttaccg acctcaacag ccgtaacccg
     5761 caggtggctt cacgcctgat tgagccgctg attcgcctga aacgttacga tgccaaacgt
     5821 caggagaaaa tgcgcgcggc gctggagcag ttgaaagggc tggaaaatct ctctggcgat
     5881 ctgtacgaga agataactaa agcactggct tgatactttt gagccaaaat gccggatgcg
     5941 acgcttgacg cgtcttatca ggcctacaag tccgttgaaa atgctggttt tgtaggctgg
     6001 ataaggcgtt cacgccgcat ccggcagccg tgcccaaatg cctgatgcga cgcttgacgc
     6061 gtcttatcag gcctacaatc cgttgaaact gctggttttg tgtgccacat ccggcatatt
     6121 aaccatgacg ccgaatcggt tgttcagagt gcccacgctg catcacccgt tgcaaaactt
     6181 ccgcttccag ctccgctaat cgcaccgacc ctaaccggcg cggacggggg atatccaccg
     6241 tcaaatccag accaattttt ccctcttcaa ttaacaatac ccggtcagcc attgctaccg
     6301 cttcgctcac atcatgcgtc accaacagta cggtaaagcc gtgctcctgc caaagtgaca
     6361 caatcaaatc ctgcatctcg agccgagtca gggcgtccag cgccccgagc ggttcatcaa
     6421 gcaataataa acccggtcga tgaattaacg ctcttgccag cgccacacgc tgtttctgcc
     6481 cgccagaaag tgcggcaggc cattcccctg cgcggttctc cagtcctact gcagccaacg
     6541 cccgacgcgc ggcatcgcgc cactggcctt taaggcctaa cccaacgtta tcaatcaccg
     6601 atttccacgg tagcagacgc gcatcctgaa acatcattcg cgtgtcttcc tgaatctcag
     6661 ccagcggtgt ggtgcccgct aacacgtcgc ctgcggttgg cgtttccaga cctgccagca
     6721 ggcgcagcag ggtacttttg ccaccaccgc tgcggcccac caccgccaca aactgacctg
     6781 ccggaatatg taaatccagt tggttcagga cgatattttc cgcgtattgt ttgcttactg
     6841 cattgagcaa caatggtgtg ccctggttca gacgagcagt attcataccg tggcctcctt
     6901 caaatgataa gccgggttcc agcgcaacca gagacgctct aacagctgcg cgctgacgtc
     6961 agcaagtttg ccgagcaggg cgtaaagaat aatagcgact accaccacgt ccgtttgcag
     7021 aaactcccgc gcattcatcg ccagataacc aatgccggaa ttggcagaaa tagtttcggc
     7081 aacgatcagc gtcagccaca tcaggcccaa agcaaaacgc acgccgacca taattgaggg
     7141 cagggcgcca ggcaggatca catggataaa cagcggtatg ccggataatc catagctacg
     7201 cgccatttcc accagaccgc gatcgatatt acggatgccg tgccaggtgt tgatataaat
     7261 ggggaatagc gtgccgagcg ccaccagaaa gatttttgcg gattcatcaa tgccaaacca
     7321 caaaatcacc agcgggatca gcgccagatg cggcacgtta cgcaacatct gaatggaggt
     7381 atccagcagt cgttctcccc agcgagatag cccgctaatc agtcccaaaa tcaaccccag
     7441 cgatccgcca attgaaaagc caatgagcgc ccgccaggag ctgatcgcca gatgctgcca
     7501 caattcgccg ctggcggaga gcgtccagaa cgccatcacc actccttccg gtgagggcaa
     7561 aatacgcgtt gacagccagc caactgagga ggccagttgc cacaccgcca cgatgcccac
     7621 cggtaaaaac cagggggcaa cgcgcaataa ccactttttc actggcgttg ccattatcgt
     7681 attccttagc tttgcgcgac gttacggggg ataaaatcat ttgccaccgc ttcgccttgc
     7741 ggattcagcg gctgcggctg gggaatttcc gggatggcga catccagatg cgggaacagc
     7801 aactcgccaa cccgatacgc ttcttccaga tgcgggtagc ccgaaagcac aaagctgtcg
     7861 atgccaagcg cggcgtattc gttgattcgc gcagcgaccg tcggaccatc gcccaccagt
     7921 gccgtcccgg caccgccgcg cactaagcca acgcccgccc ataaattggg gctgatctcc
     7981 agattgtcgc gcttgccgtt atgtagcgcc gccattcgct gttgtcctac ggaatccgtc
     8041 cgggcgaatg cggcttgtgc tttggcgata gtctcatcat caagatgcga gattaaccgc
     8101 tcggcggcct gccacgcttc gtcgttggtt tcacgaacaa tcacatgcag acgaataccg
     8161 aaacgaattt tgcgtccatg cgcggcagct ttcgcccgca cttgttcgat tttctcttta
     8221 accagttccg gcggttcgcc ccaggtgagg tagagatcaa cttgttctgc cgccagctcc
     8281 tgggcgacat ctgacgatcc gccaaagtaa agcggaggat acggctgttg aatcggcggg
     8341 aagagcagtt tggctccgcg cacatgaata tgtttaccgt tgaaattgac ggtttccccc
     8401 agcaataagc gccgccagac ctgggtaaat tccgccgagg cttcgtagcg ctcgctatga
     8461 tcaaggaaca cgccgtcgcc tgccagctct tgtggatcgc tgcctgtgac cagattaaac
     8521 aacgcacgac cattcgagag acggtcaagc gtggcggcct ggcgggcggc aacggtaggt
     8581 gaggttacgc tgggacgcag ggcgacaaga aacttcagcc gttgcgtcac cgggatcatc
     8641 gacgcggcaa ccagccacgc atcttcacac gatcgtcctg ttgggatcag cacaccggta
     8701 taacccagac gatccgccgc ttgcgcaatt tgttgcagat aaccgtgatc aaccggacgt
     8761 gaaccttctt ccgttcccag atagtgcccg tcaccatggg tcggtaaaaa ccagaacata
     8821 ttcagactca taattgtttt ccttccagtt gagtgggctg ccagatgcgc tggcgaatat
     8881 cgactttttt cggcaccaga cggttttcat aaaacagatc tgccgtttgc tgctgtaagg
     8941 cggcaacttc agcgctcaac ggtttgatgg tggtgggagg acgatgatcc agatatgagg
     9001 caataaccgg tgccggtaag cccatcgttt ttgccagtaa agcgatgctt tgttcgcgct
     9061 ggctgcgggt taacgcatcg gcctcactaa aggttgtcag cacaccctga ataaaagcgc
     9121 cgtttttttc tgcatacgga cgagctgcca gataaaacga tccagtttga ttgagatcgg
     9181 tgccgtcttt cagcacccgc acgccgccct gtaataatgc agcggagtag tagggatccc
     9241 agatagccca ggcgtcaacg ttaccttgct ggaacgcggc gcgggcatcg gcaggcgtca
     9301 ggtaagtggg ctgaatatcc gtgaatttaa gcccggcctg gcgaagggct cgcagtaaaa
     9361 gattgtgcga actggaacct ttctgaaagg caactttgtg acctttaaga tcggctacgg
     9421 ttttgatcgg gctgttttct gccaccagaa tcacttcggc tttgggtttc ggtggctcga
     9481 cgcccacgta taccaaatca gccccggcag cctgggcaaa gattggcgga atatccccgg
     9541 tactgccgag atcgatactg ccaacgttta acgcctccag catctgtggg ccagcgggga
     9601 actccaccca ggagattttt gtttgcggat agcgtttttc cagtaactgg tggctttttg
     9661 ccagcaccat accaatactg cctttttgat agcctatacg taacgcctct ggcgaagact
     9721 ccgcggcaac agcaagtgta gagacgctaa gtaaacccgc cagcgccagt ttaatgatct
     9781 tacgcatggg cattacctcg cagagacaga aggtcaggaa cctgaacatc gcggcggtgc
     9841 aacgcctgcc agaaagtttc tagcgcggta tcaagacgag tttgcagatt tggcgtgaac
     9901 tggggtttgt gttggtaatc aattacttgc gagtcatcgg caaacacgcc gtgcaggatc
     9961 tcctgggctt tcagcgtact taaaactggc ttaagggcat aatcgaccgc cagcagatgg
    10021 gccacggtgc cgcctgtcgc cagcggcagc accactttgc cttgcaacgc acgttctggc
    10081 agcaggtcga gcagggtttt cagcgcgccg gaataggcgg ctttatacac aggcgtggcg
    10141 acaatcagcc catcggcctg ttgcagctgt tcggtgaagg tcttgagtgc cggactatcg
    10201 aaacgagcat aaagcagatc ttccgggacg aagttttgca aattccagtg ataaacctct
    10261 acatccaggc catttagttt ttcccgcgca tattccagca aggagctgga gcgagaagga
    10321 aagcgaggac tacccgccag ggtgatgaca cgcatactct ctccttataa ccaattgttc
    10381 ttgttttgtt aacattgata acaattcggt cagtctgaca gagggcgtcc gcgagtcgaa
    10441 atgatttatc tgaggaagaa aagccaaaaa aagcataaaa aaagcgcaat aagaaaacgt
    10501 ttgctattta ttgccgcagg tcaattccct tttggtccga actcgcacat aatacgcccc
    10561 cggtttgcac accgggaatc caggagagtt catgtactac cccttcgttc gtaaagccct
    10621 tttccagctc gatccagagc gcgctcatga gtttactttt cagcaattac gccgtattac
    10681 cggaacgccg tttgaagcac tggtgcggca gaaagtgcct gcgaaacctg ttaactgcat
    10741 gggcctgacg tttaaaaatc cgcttggtct ggcagccggt cttgataaag acggggagtg
    10801 cattgacgcg ttaggcgcga tgggatttgg atcgatcgag atcggtaccg tcacgccacg
    10861 tccccagcca ggtaatgaca agccgcgtct ctttcgtctg gtagatgccg aaggtttgat
    10921 caaccgtatg ggctttaata atcttggcgt tgataacctc gtagagaacg taaaaaaagc
    10981 ccattatgac ggcgttctgg gtattaacat cggcaaaaat aaagatacgc cggtggagca
    11041 gggtaaagat gactatctga tttgtatgga aaaaatctat gcctatgcgg gatatatcgc
    11101 catcaatatt tcatcgccaa ataccccagg attacgcaca ctgcaatatg gcgaagcgct
    11161 ggatgatctc ttaaccgcga ttaaaaataa gcaaaatgat ttgcaagcga tgcaccataa
    11221 atatgtgccg atcgcagtga agatcgcgcc ggatctttct gaagaagaat tgatccaggt
    11281 tgccgatagt ttagttcgcc ataatattga tggcgtgatt gcaaccaata ccacgctcga
    11341 tcgttctctg gttcagggaa tgaaaaattg tgatcaaacc ggtggcttaa gtggtcgtcc
    11401 gcttcagtta aaaagcacag aaattattcg ccgcttgtca caggaattaa acggtcgctt
    11461 accgatcatc ggtgttggcg gcattgattc ggttatcgct gcgcgtgaaa agattgctgc
    11521 gggggcctca ctggtgcaaa tttattctgg ttttattttt aaaggtccgc cgctgattaa
    11581 agaaatcgtt acccatatct aattatttct tcgaattcct ctttataaga taatcagggc
    11641 tttattttaa gccctggttg ttttatattc atcgctgttg cttatttaga cattttgtac
    11701 ttttattgtt gaggttatta agcgaagcga caatggattg tgttgctgcg gttttatagg
    11761 gtaggggaga ggcagatgcg aattaaacca gacgataact ggcgttggta ttacgatgaa
    11821 gagcacgatc gaatgatgct cgatttagcc aatggtatgc tatttcgctc acgttttgcg
    11881 cgcaaaatgt tgactccaga tgccttttct cccgcaggct tttgcgttga cgacgccgcg
    11941 ctctatttct catttgaaga aaaatgccgc gattttaatt tatctaaaga acaaaaagcc
    12001 gaactggtgc ttaatgcgct ggtagcaatt cgttatctca aaccgcaaat gccgaaaagc
    12061 tggcattttg tttcccatgg tgaaatgtgg gtgcccatgc cgggcgatgc ggcctgcgtt
    12121 tggttgagcg acactcacga gcaagtcaat ttgctggtcg ttgaatctgg cgaaaatgcc
    12181 gcactatgcc tgctggcaca accctgcgtt gttattgcgg gtcgcgcgat gcagctgggc
    12241 gacgccatca aaatcatgaa cgacaggctg aaaccgcagg ttaatgttga cagcttcaac
    12301 ctcgaacagg cagtctaacg cgtcagctta agcgcagtct tcggtacaca gctacagcaa
    12361 agaatggtgc catcatcgcc cattgctgat tttttcagcg gcgtgacttc gccttctaaa
    12421 agctgaacac ggcaacttcc acaaatgccc gcgcggcaag aataagggat acgaattccc
    12481 tgattttcta attgctccag caacacctgc tggttatttc cacgaaatgc ctgtccctgc
    12541 cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt tgacggtatc atcagcggcg
    12601 gctgcgccgt aaattttagc cggagccgtt gccagaactt ccacctcatc gccaacgcga
    12661 attacgccgc tattacgggc aattaaattc tgaccaaaat cgacatcgcc gttatcctgg
    12721 gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg ccggatgttt ttgccctttt
    12781 tctggactga cggtggtgaa aatacagcgg ctacaaggtt taaccacatc aaacaccaca
    12841 tcaccaatgc gaatcacttt ccagctatct tcttcccagg ctgacgcgcc ggaaaccacc
    12901 agattggggc ggaattgctc catttttaca ctggccggac aacgttgttg gagatcacgt
    12961 aacgaggctt cgttagcaag aaggtaagga tagccatcag caaatgacag gggtacagtg
    13021 ttgtggcgtt tcacgcgccg cgtcatttgt ggccccaccc agcgcaactg cacttcgcgg
    13081 gagaaaaatc cacttagcca tttgttgatc gcgtctggtg caattcgcgc ggtaaaatgt
    13141 gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg aacatatgcg
    13201 ctactgccat ctggtgcggt gagatgcaag ccatcatgca caggtgaagg agtaaaccgt
    13261 accatctggg gaaactggcg agcggtaata aacgtaccgt caggttccgt gatcataaag
    13321 atgcgatcga aggccagacc actgacatct gccagggcat gtgtaagacc aatgccgcgc
    13381 atcgatttaa caggatgaat aaaaagccgg gttaatgtcg ccacggtgcg gtcctcaaat
    13441 gaaaataagc cctcaacttt atgacatgac gcgcttatta gctataatgc gcaacaattt
    13501 tcttagcgcc tgtcccatta ggctatttta tttgtcagtt tggcccaggg aagtgctcga
    13561 aatcctcacg tacttgtgta cgctccggtt tctccgcgct gtccatgtcc agactgactg
    13621 caacaattac gcctactgcg gtaggctctt agagtaaaag tgacgatatg aattctctgt
    13681 ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa aacctggggg
    13741 ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa gggcgacaca cggcttgttt
    13801 accagagcct gatgtggagc cgcctggcct cgcgtattat gttgccgctg ggcgagtgta
    13861 aggtttacag cgatttagac ctctatctcg gtgttcaggc gatcaactgg acagagatgt
    13921 ttaatcctgg cgcgaccttc gctgtccact tcagtggttt gaatgacacc atccgcaaca
    13981 gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcacg cggaaaaatc
    14041 tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc tggctgcata
    14101 aagaaaccgc cagtattgcc cttgatctca gtggtgatgg tttacatctg cgtggctatc
    14161 gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc agccgcgatt gttatgcgat
    14221 ccggctggca gccaggaaca ccgctgctcg atccgatgtg tggttccggt acgttgttga
    14281 ttgaagcggc gatgctggcg accgatcgcg caccaggctt gcaccgtgga cgttggggct
    14341 ttagcggctg ggcacagcat gatgaagcca tctggcagga agtgaaagca gaagcgcaaa
    14401 cccgcgcccg taaaggcctg gctgagtata gctctcattt ttacggttcg gacagcgacg
    14461 cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc ggggattggt gaactgatca
    14521 cctttgaagt gaaagatgtc gcgcaactga ccaatccgct gccgaaaggg ccgtacggta
    14581 cagtgttgag caacccgcca tacggtgaac gtctggacag cgaaccggcg ctgattgcgc
    14641 tgcatagtct gctgggccga atcatgaaaa accagttcgg cggctggaat ctctctttat
    14701 tcagtgcctc gccggatctg ttaagttgcc tgcagttgcg tgcagacaaa cagtacaaag
    14761 cgaaaaacgg tccattagac tgcgtgcaga aaaattacca cgttgccgaa agcacaccag
    14821 acagcaaacc ggtaatggca gcggaagact acgccaaccg tctgcgtaag aacctcaaaa
    14881 aattcgagaa gtgggcgcgt caggaaggca ttgaatgcta ccgcctgtac gacgccgatc
    14941 taccggaata caacgttgca gttgaccgct acgccgattg ggtggtggtg caggagtatg
    15001 cgccgccaaa aactattgat gcgcacaaag cgcgtcagcg tctgttcgat attatcgctg
    15061 caaccatttc ggtattgggg attgcgccaa acaaactggt gctgaaaacc cgtgaacgcc
    15121 agaagggcaa aaatcagtac cagaaactgg gcgagaaggg cgagtttctc gaagttaccg
    15181 aatataacgc tcacttgtgg gtgaacctga cggattacct cgataccggt ctgttcctcg
    15241 atcaccgtat cgcccgtcgt atgctcggtc agatgagcaa aggcaaagat ttcctcaacc
    15301 tgttctctta taccggcagc gccaccgtgc acgcgggatt aggcggtgca cgcagcacta
    15361 ccaccgtgga tatgtcgcgt acttatctgg agtgggcaga acgcaacctg cgtctgaatg
    15421 gcctaaccgg gcgtgcgcat cgcctgattc aggccgattg cctggcgtgg ctgcgtgagg
    15481 caaatgaaca gttcgatctg atttttatcg atccgccaac cttctctaac tcaaaacgaa
    15541 tggaagatgc gtttgatgtt cagcgcgatc atctggcgct gatgaaagat ttgaaacgtc
    15601 tgctgcgtgc tggtgggacg atcatgttct cgaacaacaa acgtggcttc cgtatggatc
    15661 ttgacggact ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa acgctctccc
    15721 aggatttcgc ccgtaaccgc cagatccaca actgctggct gattaccgca gcctgaaagg
    15781 aatagtaatg tcattaatca gtatgcatgg cgcatggctg tcgttcagcg acgcgccgct
    15841 tctcgataac gcagaactgc atatcgaaga taacgaacgt gtttgtctgg tgggccgcaa
    15901 cggcgcaggc aaatcgacgt taatgaaaat cctcaaccgt gaacaagggc tggatgacgg
    15961 tcgcattatt tacgagcaag atttgattgt agctcgtctg caacaggatc cgccgcgtaa
    16021 cgttgagggg agtgtttatg atttcgttgc cgaaggcatt gaagaacaag cggaatacct
    16081 gaaacgctat cacgatattt cgcgcctggt gatgaacgac ccgagcgaga aaaatctcaa
    16141 cgaactggcg aaggttcagg aacagttgga tcaccacaac ctgtggcagc tggaaaaccg
    16201 cattaacgaa gtgctggcgc aactggggtt agatcctaac gttgcgctgt cgtcgctttc
    16261 cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg gtgagcaatc cgcgcgtatt
    16321 gctgctcgac gaaccgacta accacctgga tatcgaaacc atcgactggc tggaaggatt
    16381 ccttaaaacc ttcaatggga cgataatttt catctcccac gaccgttcgt ttatccgcaa
    16441 tatggcgacg cgcattgttg atctcgatcg cggcaagctt gtgacctatc ctgggaatta
    16501 cgaccagtac ctgctggaaa aagaagaagc cctgcgcgtg gaagaactac aaaatgccga
    16561 gttcgatcgc aaactggcgc aggaagaggt gtggatccgc caggggatca aagcacgccg
    16621 tacccgtaat gaaggccgcg tacgcgccct gaaagcaatg cgtcgtgagc gtggcgaacg
    16681 tcgcgaagtg atgggtaccg caaagatgca ggtggaagag gccagccgct ccggtaagat
    16741 cgttttcgaa atggaagacg tttgctacca ggttgacggt aagcaactgg tgaaagattt
    16801 ttctgcccag gttctacgtg gcgacaaaat tgccctgatt ggtccgaatg gatgcggcaa
    16861 aaccacgctg ctgaaactga tgctcggtca gcttcaggcg gacagcgggc gtattcacgt
    16921 tggcaccaaa ctggaagtgg cttatttcga tcagcaccgt gcggaactgg atcccgataa
    16981 aacggtgatg gataaccttg ctgaaggtaa gcaagaggtg atggttaacg gcaagccacg
    17041 tcatgttttg ggctatttgc aggacttcct gttccatccg aaacgggcga tgacgccggt
    17101 acgtgcgctt tctggcggtg agcgtaaccg cttgctgttg gcgcgtttgt tcctcaaacc
    17161 aagcaactta ttgattcttg acgaaccgac caacgatctt gatgttgaaa cgctggaact
    17221 gctggaagaa ctgatcgaca gctaccaggg cacggtattg ctggtgagcc acgatcgtca
    17281 gtttgtcgat aacaccgtta cagaatgctg gatcttcgaa ggcggcggta aaattggtcg
    17341 ttatgtcggc ggttatcatg atgcccgtgg tcagcaagaa cagtatgtgg cgctcaaaca
    17401 gcctgcggtg aaaaaaatcg aagaagccgc cgcgccaaaa gcagaaactg taaaacgcag
    17461 cagtagcaaa ctaagctata aattgcagcg cgaactggag cagctaccgc aattgctcga
    17521 agatctggag gcgaagctgg aagccctaca gacgcaggtg gcggatgctt ccttcttcag
    17581 tcagccgcat gagcagacgc aaaaagtgct cgccgatatg gctgctgcag agcaggagct
    17641 ggagcaagcc tttgaacgct gggagtatct tgaagcgtta aaaaatggtg gctgatcgca
    17701 aaatagtcga tttaaacaga gacagctgac aacggtaaaa ttgcctgatg aactgggttt
    17761 atcaggcatg aaaaccaaca ctttgttagt catctgtaaa atagcgcatc attaaggagt
    17821 accaatgtgc gaacatcatc atgccgcgaa gcacatcctg tgctcgcagt gtgacatgct
    17881 ggtggcgtta ccgcgccttg agcatggtca gaaagcggca tgtccccggt gtggcacaac
    17941 gttaaccgtg gcgtgggatg cccctcggca gcgtcccacc gcctatgcgt tggctgcact
    18001 gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat atgaacgttg cgggagtcac
    18061 cagtgaaatt acattactgg aaattcccgg cgtgcttttt tctgaggact acgccagcct
    18121 cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg ttttgtctga taaccattct
    18181 gttactggtg aatcgcgcag aattaccggt ccgtttaaaa gagcaactgg cacgggtgct
    18241 ttttcaactc aaaacctggg gaatggcgga gattttcctc gcgggtgtgc tggtcagttt
    18301 cgttaaactg atggcttacg gcagcattgg cgtaggcagc agctttctcc cctggtgttt
    18361 attttgtgtc ctgcaactgc gtgcttttca gtgcgttgat cgtcgctggt tatgggacga
    18421 catcgccccg atgccagaac tgcgccagcc gctaaaacct ggcgtcacgg ggatacgcca
    18481 ggggttacgt tcgtgctcct gttgtacagc aattcttcca gctgatgatc cggtgtgtcc
    18541 gcgctgtggt actaaagggt acgttcgtcg tagaaacagc ctgcaatgga cactcgcgct
    18601 gcttgttacg tccatcatgc tgtatcttcc ggctaatatt ttgcccatca tggtgacgga
    18661 tttattaggc tcgaagatgc catcgacgat tctcgctggg gtcatcctgt tatggagcga
    18721 aggatcttat cccgtcgctg cggttatctt tctggccagt attatggtgc caacgttaaa
    18781 gatgatcgcc atagcgtggt tgtgttggga tgccaaaggg catggcaagc gcgacagtga
    18841 aagaatgcat ttgatttatg aagttgttga gtttgtaggc cgctggtcga tgattgacgt
    18901 tttcgttatc gcggtgctct cggcgctggt gcgtatggga ggtttaatga gtatttatcc
    18961 ggcaatgggt gcattaatgt ttgctttagt cgtcataatg acaatgtttt ctgctatgac
    19021 gtttgacccg cgtttgtcgt gggatcgtca acctgaatca gagcatgagg agtcctgaca
    19081 gtatggaatc taataatggg gaagccaaaa tccagaaagt gaagaactgg tctcccgtgt
    19141 ggatatttcc tatcgtcacg gcgctcattg gggcctgggt tcttttttat cattacagcc
    19201 atcaggggcc ggaagtgacc ctgatcaccg cgaatgcgga aggaatagaa ggtggtaaaa
    19261 ccaccattaa aagccgtagc gttgacgtcg gcgtggttga aagcgccaca ctggctgatg
    19321 atttgacgca cgttgaaatc aaagcgcggc tgaattccgg tatggaaaaa ttgctgcata
    19381 aagataccgt cttttgggtg gtgaaaccgc agattggtcg agaagggatt agcggcctgg
    19441 gaacgctgct gtctggtgtt tatatcgaac tgcagccagg cgcgaaaggc agcaaaatgg
    19501 ataaatacga tttgctggac tcaccaccgt tggccccgcc tgatgcgaaa ggtatccgtg
    19561 tgattctcga tagcaaaaaa gccgggcagc tctcgccagg agatcccgtg ctgttccgtg
    19621 gctatcgggt aggttcggtt gaaaccagca cctttgatac gcaaaaacgc aatatcagct
    19681 atcaactgtt catcaatgca ccttatgacc gactggtgac cagcaatgtt cgcttctgga
    19741 aagatagtgg cattgcggtt gatttgacgt cagcgggaat gcgtgtggag atgggttcat
    19801 tgacaacgct gcttagtggc ggtgtcagct ttgatgtgcc ggaaggtctg gatttagggc
    19861 agccagtggc accgaaaaca gctttcgttt tgtatgatga tcagaagagc attcaggatt
    19921 cgttgtacac cgatcacatt gattatctga tgttctttaa agattcggta cgcggtctgc
    19981 aaccgggagc tccggtagaa ttccggggta ttcgcctggg taccgtaagc aaagtgccat
    20041 tctttgcgcc gaatatgcgt cagacattta acgatgatta ccgtattccg gtactgattc
    20101 gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa tgcggatgtt gttgagcacc
    20161 ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa aaccggaaac ctggtcactg
    20221 gcgcactgta tgttgatctc gatttctatc caaatacgcc tgcaataacc ggtattcgtg
    20281 aatttaatgg ttatcagatt atcccgaccg ttagcggcgg tctggcgcaa atccagcaac
    20341 gactgatgga agcgttggat aagatcaaca aactgccatt gaatccgatg attgaacagg
    20401 caaccagtac gctttctgaa agtcagcgca caatgaaaaa cctgcaaacg acgctggata
    20461 gcatgaacaa gatcctcgcc agccagtcga tgcagcagct gccgacggat atgcagtcaa
    20521 cgttgcgtga attgaatcgc agcatgcagg gcttccagcc tggctccgca gcctacaaca
    20581 agatggtggc ggatatgcag cgccttgatc aggtgttgcg agaactgcaa ccggtgctga
    20641 aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc gaaggacaaa aaagatccag
    20701 agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg attgcggcac tgtggctggc
    20761 cggatgcagc tccggcgaaa ttaataaaaa ctattaccag ttacctgtgg tgcagagcgg
    20821 tacacaaagc accgccagcc agggtaatcg cctgttatgg gttgagcagg tcgctgttcc
    20881 tgactatctg gcggggaatg gtgtggttta tcaaaccagt gatgtgaagt atgtgattgc
    20941 caacaacaac ctgtgggcca gcccgttgga tcaacagttg cgcaacactc tggttgccaa
    21001 cctgagcacg caactgcccg gctgggtggt tgcctcccag cctctgggaa gcgcccagga
    21061 tacgctcaat gttaccgtaa cggagtttaa cgggcgatat gatggcaagg tcattgtcag
    21121 tggtgagtgg ctgttgaacc accagggaca actgatcaaa cgtccgttcc gtctggaagg
    21181 ggtgcaaact caggatggtt acgatgaaat ggttaaagtg ctggccagtg tctggagtca
    21241 ggaagccgct tctattgcgc aagagataaa gcgtctaccg taatcataaa gatttgtaaa
    21301 tataaccgtc tccggtatgt tgcctgaggc ggtttttttg tttctaaggt gcggaaaaag
    21361 ttgttcctct tcacattttt tgtacaactg acatgcccgt gtagctcaca aatatgacag
    21421 tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg gtattgctta actgtgattg
    21481 cacatttagt aatcactgtt ttcttttcca ccagaaacca gtatgaggga aacgaggcat
    21541 gaagagacaa aaacgagatc gcctggaacg ggcacatcaa cgtggttatc aggccggcat
    21601 cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg aatcaaaggt cacaatggct
    21661 gggaggctgg cgagaagcca tggcggacag ggtagtaatg gcctgattct gtctctttaa
    21721 aaagaaacct ccgcattgcg gaggtttcgc cttttgatac tctgtctgat tataatcaga
    21781 aggcagacgt atcctggaac agaccgactt tcaggtcgct ggcggtatag atcagacgac
    21841 catcaaccag cacttcgcca tctgccagac ccataatcag acgtcgatta acaatgcgtt
    21901 taaagtgaat acggtaggtc acttttttcg ctgtcggcag tacctgaccg gtgaatttca
    21961 cttcgccaac gcccagcgcg cggcctttac cttcgccgcc cagccagccg aggtagaacc
    22021 ctaccagctg ccacattgcg tccaggccca ggcatcccgg cataaccgga tcgccaatga
    22081 agtggcatcc gaagaaccac agatccggat tgatatccag ttctgcttca acataccctt
    22141 tgtcgaagtt accacccgtt tcggtcattt tgaccacacg gtccatcatc agcatgttcg
    22201 gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc gcgaccagag gcaagaaggt
    22261 cttcttttgt ataggattcg cgtttatcta ccatgttctc tgtaagcctt attttattga
    22321 agcacgcagg atagctaaca cgtgtacgct gaacaagtcc gatcagttcg gaataaacca
    22381 gttcagccaa cgcaatggcc atggaaaacg gtgacgtcct tcctgttgcg atgcttgcgc
    22441 gatacgttcc tggatggttt gcatcagcgt cgtttggcct tcgccatccc acaccagatt
    22501 taataataac ggcagtgcgt cagtcacatc gtctactgcc cagatggtga atttgccttc
    22561 ttctaccgct ttcaccagtt cactgtgaag acttaaatgg cgaatgttag cagtggggat
    22621 aatgacacct tgtttcccgg ttaactcacg ttgctggcaa atagcaaaga agccttcgat
    22681 tttctcattt aaaccaccga ccggctgggc acgaccgaac tgatcgactg aacctgtgat
    22741 agcgatactc tgattcaccg gcacatcggc gagggcgctt atcagggcgc agagttcagc
    22801 catcgaggca ctatcgccat ccacttcact gtatgactgc tcaaatgtca gcgatgctga
    22861 gaaggggatc tgttgctcaa gctgtagttc cgacatcagg aacgcctgca tgatcatcat
    22921 ccctttcgca tggatattgc cgccaagctc cgctttgcgt tcgatgtcgg tgaactcacc
    22981 atcgccaata tgcacaacgc agctaatgcg agaaggttcg ccaaaagcgc gtggatgacc
    23041 cggaaattca atgaccgaaa gggcgttaat ttgcccgatg cgttcgcctt cggtttcaat
    23101 caggatttgc tcctgaagaa tctcatcctg catgcgttca gcgaggaaac cttcacgcca
    23161 ttcacgctgc tgcagcatta agtttagctg ctcgccggag aaggtgtcgc catcgcacag
    23221 ggaggcgacc tctttacact ggcggaggat ccactgcggg ctaagcggaa gtgtttcttg
    23281 ttcaccggtg tagcgggctg cttcgcggat aagtaccggc caggcatccg ctcccggtgc
    23341 aggtaagtga ttatgtcttg cggtcaatgt tacccagcga caccactggc ttactgactc
    23401 cgcatcgaca atctgcagag tatcttcaaa ttcgctataa atagcctgct ctgaaagctc
    23461 tggttccatc tcctggaaat cagccaatga ttcgcgttcg cctaccagaa tgactttcag
    23521 cttcaatggc atcgaaggca cagagacggg gagagggcgc gactcatcaa acgcaaccca
    23581 gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc cacagcagag gttgcgccag
    23641 cagtgtacgc aaagagatga tgagaatacc gccatttgcc tgatgcacca gacctggctg
    23701 cagggtaatg tcgccattaa actggcgcag gcagccaaag agttgttccg cttctaccca
    23761 gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca tctgcactca ctgcgttacg
    23821 taagcggatg gtgtgcccgg aaacctcata gtgaccgccc accagttggc ctgcatcgct
    23881 ttgtagcgca cgcgcggcat cggcaataag attcagatac tcagactctt ccggggcctt
    23941 cgccagcata aaagaggagg atgctcgcgt atgcagcaac tgctccagcg caaattgcag
    24001 tcgcggttga gtatcactga ataaaggatc gttttcgtca atcaaatgtg gctgagcaaa
    24061 tatttcctga tagctatcgg tatcaggaac caggtcacgc catgcaagtt tcgtaatggt
    24121 caaagttgat gttttttagt ctgttgtcaa agccgcgatt ataccgtaac cggcactaca
    24181 gcacacgtag aaagcaccga caatactcct ggcatgggcg ttaaagctca caggatggag
    24241 attcttttct tcactggccc aaaaagctga tattctgtaa agagttacac ggtaacattg
    24301 agatcgctat gaaatatcaa caacttgaaa atcttgaaag cggttggaaa tggaagtatc
    24361 tggtgaagaa gcaccgcgaa ggggagttaa tcacccgtta catagaagcc agtgccgccc
    24421 aggaagccgt cgatgtgttg ctctcgctgg aaaatgaacc cgtgctggta aatggctgga
    24481 ttgacaagca tatgaatccg gaactggtca atcgcatgaa gcaaaccatt cgggccagac
    24541 ggaagcgcca ttttaatgcg gaacatcagc atacgcgcaa aaaatcgatc gatctggaat
    24601 ttattgtctg gcaacgtctg gctggtcttg cgcagcgtcg cggtaaaacg ctttctgaaa
    24661 cgattgttca gctgattgaa gatgcggaaa acaaagagaa atacgcgaat aaaatgtctt
    24721 ctttgaagca ggatctgcag gcattgctgg gtaaggaata actgacgaaa gtcagttcaa
    24781 ttttctaaag acgaaaaaaa accccgcagc agcggggttt tttaccagac gagaacttaa
    24841 gcctgcggct gagttacaac gtctttgatg cctttaactt cgatctctac gcgacgatcc
    24901 ggagccaggc aatcgatcag tgcagcacgc tgtttcacgt tgtcacaggt gttgccagta
    24961 accgggttgg attcgcccat accacgtgcg gagattttgt ctgccgggat acctttagag
    25021 atcaggtaat caacaacgga ctgagcacga cgctcggaca gagcctggtt ataagcgtca
    25081 gaaccgatgc ggtcagtgta acccagaaca actacggaac cgtctttcgg atccaggttg
    25141 ctcagctggc tgtacagctg atccagagca gcctgacctt ccggtttcag ggttgctttg
    25201 ttgaaggtga acagaacgtc agacttcaga gtgaagtgct tggtctgtac ttccggtgcc
    25261 ggagctggag ccggagcaac tactggagct gcttcgccct gaccgaaacg gtaggaaaca
    25321 cccaggctca gcatgccgtt gtccggacga gtgccgatgg tgtgtgcgtc accgatgttg
    25381 ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcata ctcaacaccg
    25441 ccagcgaaga ccggagaaac gccggtgtcg tggtctttag tggatgcgcc accaggtacg
    25501 ttagacttgg tgtctgcacg ccataccata ccacccagac gagtgtaaac gtccagatcg
    25561 tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt gtatgcgccg
    25621 ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat ttcaaagcca
    25681 acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt ttcgtgggtc
    25741 ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag tttagcacca
    25801 gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc agccagtgcc
    25861 actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc caaaatacgc
    25921 catgaatatc tccaacgaga taacacggtt aaatccttca ccggggatct gctcaatatt
    25981 aactctaccg atatcttcgg cttatgccga gcacccctgg cgatgtaaag tctacaacgt
    26041 agttgaaaac ttacaagtgt gaactccgtc aggcatatga aaaaaaagtc ttgtataagg
    26101 tacgtttaat cttttttgtc agcgacaatt tacagaagaa aatcgcggaa accgcttcag
    26161 acaagcctcc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg acaaaaaaag
    26221 ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt ttagcccgga
    26281 aagttgtctc gttgcgtgag aggatgcgct taccggacgc ataataaacc ccatagcgtt
    26341 accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat catctgccaa
    26401 ccaaccgatc accacgctgt aattgcccgt gcgtaaagcg cgaaccattg actcaacggt
    26461 gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc cagatgcctg
    26521 aacccattcg cgactcagtt tttgttgcgg tgttaaccag agttgccagc gcgattgctg
    26581 accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct gatcttcgcg
    26641 atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac gcgcaatttt
    26701 acttgctgcg gatgagaacg acgaagcacg atgtgcatag cctgaagtgt acataatcaa
    26761 tccagccctg tgggttactg tatgaatata cagtacatcc ggtgacgaca aagatcaacc
    26821 ctattttcgg aaagagcctc gcaaattttg tcgttagtga cgggaaaaca taaattaatc
    26881 ttgcccctta agaataagtt gcctattttc gtagttaacg gatccgttaa tgtgaatcat
    26941 tcttttatgt tatgatttta aaaggaattt tatgaaaagc ctctcctata agcggatcta
    27001 taaatcacaa gaatacctgg caacgttggg cacaattgaa taccgatcat tgtttggcag
    27061 ttacagcctg accgttgacg acacggtgtt tgcgatggtt tctgatggtg agttgtatct
    27121 tcgggcttgt gagcaaagtg cacagtactg tgtaaaacat ccgcctgtct ggctgacata
    27181 taaaaagtgt ggccgatccg ttaccctcaa ttactatcgg gttgatgaaa gtctatggcg
    27241 aaatcaactg aagctggtgc gtctgtcgaa atattctctc gatgctgcgc tgaaagagaa
    27301 aagcacgcgc aatacccggg aaagactgaa agatttgccc aatatgtctt ttcatctgga
    27361 agcgattctc ggggaggtgg ggattaagga tgtacgggcg ttacgtatac ttggggcaaa
    27421 aatgtgttgg ttgcgactgc ggcagcaaaa cagtctggtg acagaaaaga ttctgtttat
    27481 gcttgaaggg gccattatcg gcattcatga agctgcgctc ccggtggcac gccgccagga
    27541 gcttgcagaa tgggctgact ctcttacgcc gaaacaggag tttcctgcgg aacttgagta
    27601 atctggcgtt gcagacgacc aatctctggc agtaatgcaa tcaataaccc aacttgttgt
    27661 acgaccagag gttctttgct gtctgcccgt ggttcaagtt gctgcatccg ctgtttcagg
    27721 ccagctaatg cctgattgac gcgttcttca tcagcaggtt gatgatgtaa cgcgtcatca
    27781 acatagcaaa ctgcgtcatc aagaaacgcc agaatttcag gattagttaa ctgctcccgg
    27841 tgagcaccga gggctgagat atagctggta aacgtatggt taaggcacag caaccgaaac
    27901 gcggcttcgc gaatttgcgg ggtaacgttc ggttcgctgg acatatttga taccaccgac
    27961 gccagttcag catcacggtt gtgtgcatcg cggcgggcaa tacgatacgc cagacggtta
    28021 tcacgcccct gatggtattg ctccagtatg gcatcgagat accggcagtt ggcctcggtg
    28081 gcgcgttcga gcattcgcgg cagattgcga aacttccagt caggccagat gtagctcact
    28141 gccgcccacg caatggcaca accaatcagc gtatcgatta cgcgaggtaa cgccacttca
    28201 aaaccttcac ccagcaagtt gaaacacagt agcaccaaaa gtgtgatgaa catcgttgca
    28261 tgggcgtatt gcacgttacg gaaggcaaaa aagagcacgc cggtaataac cagcagcacc
    28321 agctgccctt ccagtgatgg cacaaaccac agcacaggaa taccaatcgc gatacctacc
    28381 agcgtaccaa taatccttaa cttcaggcgg tggcgcgtgg cgttatagtt tggctggcag
    28441 acaaacaaac ttgtcagcaa gatccaatac ccgtgatgca ttccggttat ctgaatgatg
    28501 gcgtagccga agcacaacac cagcgacatt cttaccgcat gacggaagag ggcggattcc
    28561 ggcgtgaagt gacggctaag acgcagccag atatcactca atccgtgcgg gctgtcatca
    28621 gcgagctcat tttcgtcatt attatggggc agtgcctggg cctgttctga ttcaattgtt
    28681 gccagttggg catcaatggc acgtaaattg ttcagtaaaa atcctagtgt tttgagtaaa
    28741 tcagcaggag cgccgttatc gcgcatccgc tccagcgcag catcaatatg cgtaaaagcg
    28801 cgctcaaaat gcggatcatg ttgataaggc tgacgcagca aaatacagcg tgacagttgc
    28861 tggcacgcct ggccctgcat cgacatcagc cgctgaaaac ggaacagcac gtcgctgtgg
    28921 cgaaaatgtt cacgcaatgt ttgatattga atatgagaag agctggcacg ctcgtgaata
    28981 tcctgcgcga caaagtaata atgcagtgtg cgacgcgttc ctcgttgacc acgatcgcca
    29041 cgtaagcggg tcagcagcga gagtttcgtc tgattcaatg tcgccatcag ctgaccgttg
    29101 gcgagagcca aatcgtacag cggtgcctgg ctttcatctt caatatcagg atcaaacatg
    29161 cgcgacttga gctcaagata acgcgccagt tgttcatagc aacgcgccag gttgtcctgc
    29221 agcgggcgga ccgggaacag cagatgacca ataagtgtca ggacgttgta ccagacggca
    29281 ccggccagca gatacatcgg ctgctgatac cagtgctcat acagtgacgt ccccaacata
    29341 gtgtaaatgg cgatcagtaa cgcaccgaag gcaattgttg catagcgttg acccagaccg
    29401 ccgagcaaaa tgaagccgct ggtagagagc gttaaaccaa ttgcaaatag ccagggccag
    29461 ggaaacagca attctactga ggccgaggcg ataaaaaagc agaacagcgt aatgatgagg
    29521 ttacgcaaac gtcccgccag tcggtcatcg agatcggtca gcgccgctgc caccatcccc
    29581 agcgttagcg gaatcgtcag ttttacatca cccagccacc acggaaacgc tgtggttcca
    29641 caaagcgcaa taaaaatacg cgcgtaatac agccaggcgc tgttccaggt atagcgtttg
    29701 agcaaaggac ttagcataaa ggccatatta gtggattatt caaaacgacg acgtgcattg
    29761 gcttcacgcg cagcttgcgc tgtttctacc gataccacgc gacgaccgac cggccatagt
    29821 gcaatagcgg caattttaaa gttcgcaatg ccgacaggaa tgccaatgat ggaaatacat
    29881 tgtgcgatgc ccgttgcaat gtgcatcagg cataaccacc agccaaagaa aatcagccag
    29941 aaaatattca ataccgtacc gccagtattt agcagcacat ttttgccagc cgggttcagt
    30001 tcatcgacat gaatagcttc attgccataa ggcaccagag acagtttagt gatctcccag
    30061 caggatcgtg tcagcggtaa ggtaaaaatc agcacaatac tgaccagagt cgccaacagc
    30121 cagcccagag tggtggcaaa tccgccaagc acaaagttca gaatgttcaa aacggtacgc
    30181 ataaaacctc gctttactgt ggttttcagt aatgggcggc aattgtaacg tttttttggg
    30241 ctggagcacg ttttctctga cggttacact gataagaaat aatttcgtgt ggatctacag
    30301 agtcatggaa ctgaaagcga caacgcttgg aaaacgtctg gcacagcacc cttacgatcg
    30361 ggcggtgatc ctcaatgccg ggattaaagt ctccggcgat cgccacgaat accttattcc
    30421 tttcaatcaa ttactggcga ttcactgtaa gcgcggtctg gtatggggcg agctggaatt
    30481 tgtactgccg ggcgaaaaag tggtgcgtct gcacggtacc gaatggggcg agacgcagcg
    30541 tttttaccat catcttgatg ctcactggca gcggtggagt agcgagatga gcgaaattgc
    30601 atctggtgtt ttacgccagc aactggattt gattgccacg cgtactggag aaaataaatg
    30661 gctgacgcgt gagcaaacct ctggcgttca gcaacaaatc cgccaggctt tgtcggcgtt
    30721 gccgttgccg gttaaccgac tggaagaatt cgataactgc cgtgaggcat ggcgtaaatg
    30781 tcaggcctgg ttgaaagata ttgaaagcgc tcggttgcag cataaccagg cgtataccga
    30841 agccatgctt accgagtatg cggatttttt ccgccaggtc gagtcttcac cgctgaatcc
    30901 ggcgcaggcc cgggcagtcg ttaatggcga gcattctctg ttagtgctgg ctggtgcagg
    30961 aagcggaaaa acgtcggtgc tggtggcccg tgcaggctgg ttgctggcgc gtggtgaagc
    31021 gtcccctgag caaattttat tgctggcgtt tggtcgcaaa gccgctgaag agatggacga
    31081 gcgtattcga gaacgactac ataccgaaga cattaccgca cgcacatttc atgcgctggc
    31141 gctgcatatt attcagcagg gcagcaaaaa agttccgata gtcagcaaac tggaaaatga
    31201 taccgctgcc cgtcatgaac tttttattgc tgagtggcgc aagcaatgca gcgaaaagaa
    31261 agcgcaggcg aagggctggc ggcaatggct gacggaagaa atgcagtggt cagtgccaga
    31321 aggtaatttc tgggatgatg aaaaattaca gcgtcgtctt gcctctcgcc tcgatcgctg
    31381 ggtaagtctg atgcggatgc acggtggtgc acaggcagaa atgattgcca gtgcacccga
    31441 agtgattcgc gatctgttca gtaaacgtat caagttgatg gccccgttat taaaagcctg
    31501 gaaaggtgcg ctgaaggcag aaaacgctgt cgatttttcg ggccttattc accaggcgat
    31561 tgtgattctg gagaaaggtc gctttatcag cccgtggaag catattctgg ttgatgaatt
    31621 tcaggatatc tcgccgcagc gcgcagcgtt gttagcggca ttacgcaagc aaaacagtca
    31681 gacgacgttg ttcgccgttg gtgatgactg gcaggcgatt tatcgcttca gcggtgcgca
    31741 aatgtcgctc accaccgctt tccatgaaaa ctttggtgaa ggtgatcgct gcgatttaga
    31801 cacgacttac cgttttaaca gtcgtatcgg tgaggtggca aaccggttta ttcagcagaa
    31861 cccaggccag ctgaaaaagc cgctaaacag cttaaccaat ggagacaaaa aagccgtcac
    31921 gttattggat gagagccatc ttgacgcttt gctggataag ctctctggtt atgccaaacc
    31981 ggaagagcgc attctgatcc tggcgcgtta ccatcacatg aggcctgcca gcctggaaaa
    32041 agcggcaaca cgctggccga agttgcaaat cgactttatg accattcatg ccagcaaagg
    32101 gcaacaggcg gattacgtca tcatcgttgg cttgcaggag gggagtgatg gttttccggc
    32161 tgcggcgcgg gagtcgatta tggaagaggc gctactgcca tcggttgagg atttcccgga
    32221 cgctgaagaa cggcggttaa tgtacgtggc gctgacccgg gcacgccatc gggtatgggc
    32281 actgtttaac aaagagaatc cctctccatt tgtcgaaata ctgaaaaatc ttgatgtgcc
    32341 ggtggcgaga aaaccgtaag aaacaggtgg cgtttgccac ctgtgcaata ttacttcaga
    32401 cggtccgcga gataacgctg ataatcgggg atcagaatat cgaccgcgtc gttgaaatgc
    32461 ggcgactgga taatgaagtc tgccgttgcc acgttggtgg cgaccggaat gttccatacc
    32521 gtcgccagac gcagcaaggc tttcacgtca gggtcgtgcg gcacggcgtt tagtgggtcc
    32581 cagaagaaaa tcaatacatc aattttccct tctgagatca atgcgccaac ctgctggtca
    32641 ccccccattg ggccactcaa catcgcgttg acgttcatgc cggttgcacg ggaaattaag
    32701 ttaccggtag tgcctgttgc atacagtacg tgttgttcca gtaacggttg atgccgttcc
    32761 acccagctca tcagcatttg tttgcagtga tcgtgtgcca ccagcgcaat atgtttccgc
    32821 gcaggtaaag tgcgtgtcgt cagttccata atgtacatcc gtagttaact ttcctacaga
    32881 ttactgtaag cacttatcgc tgcaagataa agaccgaaaa agcctgcgca caggcacaaa
    32941 aatctcagga agatggttgt ttttccgccc attgcaggaa agtatttcgc gtttgtgggt
    33001 cagccagttt aaaccaatac ttcagccgtt gttctgtgag cacctgagac tgcggtggaa
    33061 tagcatccag ctcggaaaca cccgaaagca gcgtactatc atctgccatc atcgtggcaa
    33121 attgcggtag tgaagcgcgt ttcgcggatt tgttatagcg ttcaacctcg acctcataat
    33181 caatcgtttt tgccgttgag gtaatggcga gaatatccag ctttaccgga atcggcgtcg
    33241 catcgccatc caacaattca aggcgcggcg cggcatcaaa atggttagct tcccgctcat
    33301 tctccagacg aggcaggcga aaattgacct ggttgatgag ctgggtatta aaactgacca
    33361 ccagcggtgg ggagatatag agccgttctt cactattgga aagatgaatc gtcttctcaa
    33421 cgcgaaacac taactgatgc ggtccgttat ccagttcaat gctgtccgca ccgcgcagca
    33481 gagagctgga aaccttcttg ccgtcgagca ccagaagatc gacatcggtt gaaagccgca
    33541 gcgtggtggc aaaaacggat accggcaaac ataatgcaat caaggtggtc acgatgccgg
    33601 ttttcatagg tagctcctgt caaaagaccg cccgaataat aatgtgtcaa aatttttctt
    33661 taatcatact tactaacata tagacatatt tcccgctttt gctctcattc attcgtatta
    33721 gctgcatggt tggcatgtcg gctttgtcgt acactttgtt aaacagtctg gaggaaataa
    33781 tgaaagaaac cgatattgca ggcattttaa cgtccactca caccattgcg ctggtggggg
    33841 caagcgacaa acccgatcgt cccagctatc gcgtgatgaa atatctactc gaccaggggt
    33901 atcacgtcat tccggtttcg ccaaaagttg ctggcaaaac actgctgggc cagcaggggt
    33961 atggcacgct ggcagaagtt cctgaaaaag tcgatatggt cgatgttttc cggaattcag
    34021 aagcggcgtg gggcgtagcg caggaagcga tcgccatcgg cgcgaaaacc ctctggatgc
    34081 aactgggggt gattaatgag caagcggcag tgctggctcg ggatgccgga ttaaatgtag
    34141 tgatggatcg ctgcccggct attgagatcc ctcgcctggg cctggccaaa taaaaaatcc
    34201 ccggaaggca aaaaccttcc ggggatttgt tcagggatta gttacgcaga cgcggggcct
    34261 ggagttgttt acggatggtc tgcgccagct catccatgga tggctgttca ggatgttcat
    34321 cctgcaattc gctacttagc tgggcttcag ccaggtaggt atgaaccggt aggccgttat
    34381 cgtcctccat caccacgtga taccacggag cggcgcgaag ctcgtcattc accgccagct
    34441 catcaggcga cggttcagag agcgaataaa ccgggtcgat atccacgacc actccgagat
    34501 aacctaacag ggaatggcgg acctgctggc cgataccgaa tttgctggca atcatagtca
    34561 cctcccggga aatctttacc tttgatataa gggtaaaatt ccacatttca agttacatga
    34621 cgcgacaggc aaaccctttc agatatagcc cttccggata ggtagcgatc accggatgat
    34681 cggctgcctg acggaactgc tctataaatt gtacatcacg accggcatca attgcggcat
    34741 ccgcgatgat tttctgaaat aaatcgctgg tcatcagacc ggaacaggag aaagtcagga
    34801 gaataccgcc ttcattcagc agctgaatcg ccagcatgtt gatgtcttta taaccacgac
    34861 acgcgcccat caactggctt ttattctcaa caaacttcgg cgggtccatc acgataacgt
    34921 caaatttttc accgcgatcg cgataagtac gcagcaattt aaagacatca tcacggacaa
    34981 actcagcctt gctcagatcc agtttgttca gctcaacgtt ctgccgtgca atatccaggg
    35041 cttcctggga ggtatcaacg ctgacaacct ggctgcaacc gcccatcagt gccgatacgg
    35101 cgaaaccacc ggtataggag aaacagttaa gtacgcgttt attttcgacg tagcggcggg
    35161 tagccaggcg gctgtcacgc tgatccaggt agtagcccgt tttgtgtccg tgctgaatat
    35221 tcaccagcag tttcatgccg tgttcttcaa tcggcagcag ggcaggcggc aactcgccgg
    35281 tgatggggcc ctgagtcagc tccatccctt cttttttacg taccgcgacg tcgctgcgat
    35341 cgtaaatggc acattccggg tacagcgttt gcagggcgct aattaatgcc gcgcgctgat
    35401 actctgcgcc agcactcagc agttgcagca ccagaaaatt accgaaacga tcgatagtaa
    35461 tacccggcag gccatcagat tctccggcga ttaaacgata gctgtcgagg ccatcttttt
    35521 gcgccagcca gtcacgccat ttttgtgctt gctgcaaacg gcgggtaaaa aaagcaatgt
    35581 cgatagattc attcgggtca aacgtccaga cgcgcgcccg gatttgcgaa gctggcgaat
    35641 aagcgccgcg tgctaaccat tttccctgat gatcaacaat atcgatggtt tcaccgaggc
    35701 tggctttacc ttccatgcgg gcaacggccc cggaaaagac ccacggatgg cgacgaagta
    35761 atgatttttc gcgccctttg gctaacacta aacgtacact cataatttac ttttctgtcg
    35821 atgcaaagaa atgggcgtca ttgtccggag ttttccatgg atttgcaacg cgtccattaa
    35881 ggataaggag agcagcaatg tcgaaagtct gcataattgc ctgggtttac ggtcgagttc
    35941 agggcgttgg atttcgctat accacgcagt acgaagcgaa aaaactggga ttagccgggt
    36001 acgccaaaaa tcttgacgat ggcagcgtgg aagtggttgc ctgcggtgaa gaagggcagg
    36061 tagaaaaatt aatgcagtgg ttaaagagtg gcggcccgcg ttctgcgcgt gtagaacggg
    36121 tgctcagcga gccgcatcat ccctcggggg aattaaccga ttttcgtatc cattaaatac
    36181 attttaccgg ttttggcagg ccagcaattt tggtggcttg cttagccgga cctttcggaa
    36241 acagtcggta taaatagcgg ctgtttcctt tttcttcgcc aaatttattc gccatcgctt
    36301 ttaccagcat acgaatcgcc ggagaagtat tgaattccag atagaaatta cgcacaaaac
    36361 gcaccacttc ccagtgttct ggcgacagcg taatcccttc gttctctgca atcaccactg
    36421 ccagcggctc actccactgg ctgctttctt tgagatagcc ttcggtatcc gtttctatct
    36481 ctttaccttc gaagatcagc ataattatta ctaccttaat cagactggcg gcagtgtaac
    36541 aaaaaacaaa gccccgcata aagcgaggct atgaaagtgt tagcgggtaa gattaatcgc
    36601 ggctggcgaa gcccagaatg ctcagcaggc tgacgaagat gttgtacagc gaaacataca
    36661 ggctaaccgt ggcacgaata tagttcgttt caccgccatg aatgatgttg ctggtttcaa
    36721 acaagatagc gccagaggag atcagaatga agaccgcgct gatcgccaga tgcagagcag
    36781 gcagttgcag gaagatatta gcaaccatac caatcagcac caccacaata cccgccatca
    36841 gcataccgcc gaggaacgac atatctttgc gggtggtcag cacatatgcg gagcagcaga
    36901 agaacactaa cgccgttccg cccagtgcca tagcgattac gtcacccatt ccggcagaca
    36961 gataggtatt cagaattggt ccgaggatat aacccagaaa accggtaaag gcgaatgcgg
    37021 agataatccc ggtcggctta ttcgccgttt tataggtcag gaacatcaaa ccatacatac
    37081 ccaccagcgt cagaatcaga cccggagatg gcagcatcag caccgtactg gcagttgcgg
    37141 taatcgccga aaaggccagc gtcaggctca gcagaaaata ggtattacgc agcaccttat
    37201 gggtgctaag cagcgatgta cggtcatgtg aagaactaac aatacgatcc attgagtcac
    37261 tctctatgac agatgtaatt aattaagcag cataatgata atgcgtaagg gcacccagaa
    37321 gttttaccca tctttacgca tttgatctgg aacaggttta acagcggatt atcaggttat
    37381 taagcaatga tataacgctc tgagaatttc gacagacaaa agaaaaaggg gttagcattt
    37441 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
    37501 gtcgccggtt ttcaagaccg aagaaaaaca aataaaaatc agtgtaatac gtgaagctgc
    37561 tgaaaattta atcaaattta acaccaagaa aaatcaaggt gttaggaagt tgctcatagc
    37621 tagtatacca agtatttttg ccagaaaatt ttctgtattg cctacttgct aaccacgagc
    37681 ggcagatcca gtgttggtgt gatttttgtt ttacgatcgt aaatcaacac ctgattttct
    37741 gttttatgcc cgctgaaaat ttgtttgtcg cgactgctgc cttcgtaatc agagatcccc
    37801 ttggctttta tatcgtgaaa attgcacccg aacggaacgc caactttttg ctcggctgag
    37861 cgtttagcct gattccacca gttgttcagc gttttagcta tgactttccc gcctttggtt
    37921 gtattgatca catattcgca tgtacaggaa gatacatttc gggctaactg gatcgctgta
    37981 cgtaatcgcg gtgaccattc cttgatttgt ttggtgccgg ttttgttttg ctcaatgtag
    38041 atccctttat ccataatatc ctgccatttc aactcaagca catcaccgag ccttgccgca
    38101 cagagatagg aaatctccat tgcaatgcgt aactgtggaa ttgcttccgc atatatcgct
    38161 gcatactgtt catcggtgat gtaaacagtg cgggctttaa gagagaattt tctgactcct
    38221 ttgcatgggt tattcttcac gtatccacgc tcatatcccc acccgtatac tcgactcagg
    38281 cttgccagtt catgatttgc ctgggtcttg ctctcaagcc ctcgtttatc catgaaaatt
    38341 cttacctgct cagttttgac attatcagca agcacttttc cgaataccat cagcaacgcc
    38401 ttctgatgtt gtcgataatc tttttgggtt cggggggcca gttctgtaaa tgcaggggag
    38461 tccataaaca tgtgccataa tttagctacg gtcattatgt tgtggagttt tgctttttcc
    38521 agttcataat tttgccagac tttagctacg ctggttttcc gcactcttcc tagccctata
    38581 gttcttgtac ttccttctgg tttccacacg taactgtaac cattcgatct aacccgcggt
    38641 ggcagtacat tatctttttt attttttctt ggtcttccca ttgttcagcg cctcaaaatc
    38701 gggttcagca gaaaccagtt cagatgtttt tggcatcgtt gtcagtccgt gtggaatatc
    38761 cctgcgtaga actattggtt cgtttttagg accgattaca aatgggatgc cgtgcagtct
    38821 taactggtgt tgctgttttg tgtatcgctc gtatttcgtg atctctttaa tctctgctgg
    38881 agatagagtt aattcgtaca tgtggtcacg tgcctttaca gcatgaccgc cgccactata
    38941 gctggtgggc ggcgatatgg gttgaacatt aaaaatcagc ctgactcggg atcagttttt
    39001 gccagatagc tgaaacgtat tttgcctgat aacgggcgtc atcaagtgca ttatggcgct
    39061 caccttcgaa tggaatagcc gttctggcat cgaagtctat ggctttcccc agctcaacga
    39121 ttgtgcgtac atcgcgatcg ttgtagtaac gccacgggca ggggatcccc tgccgttcgt
    39181 atgaacggcg caaaatcgtg ttgtcgaagt tggctccatt tccccagacc tgaacaaaaa
    39241 attcaccgga gttttcgtcg ataaattccc gcagttgtaa cagtgcatca tctaacggga
    39301 tttcatcggt cataatggca gattgcgctt cgcgtgattg cttaagccac catttaatgg
    39361 tgtcccgatc aatgactccg ccagcagttt ccagatcgat agtcttacta aattccggtc
    39421 ccatatctcc ggtttgcgga tcgaaaaata ttgcacctat tgagatgatc ggggcattag
    39481 gattttttcc catggtttca aggtcgatca ttagatggtc acacgtcctg ctggtggatg
    39541 tgatttcgtg atgaccgttc accgcaatta agggatctgc cgcttcgcca gtttcactat
    39601 cgctggtgtg atgctgatcg ttaccagcat tctcattgtt cggatgttca gcgccttcta
    39661 tttcctccgg atcatcttcc tgaacttcag cctgattttt ttcatcgaat gtttcctggt
    39721 atgttgcgtc gcccatcacc gcgccacagt caggacagtt gccgccaccg ctctgaccgc
    39781 aggcggtgca gactttttcc tcttcctgtt gcgctactgg ttcggattgt ttcgtttctg
    39841 gctcgtcttg taacgcattt tggctgtttt gttctgcttt ctggtcgttc tgttccgttt
    39901 ctagctggtt ctggttcaca gaatcgcagg tttcaatccc cttaacccat ttcgaatcat
    39961 tcgggtcgct aatcccttca acaaactcac cacgagaagc ggcaagcaat ttgtcggcat
    40021 ctggtcgact gatgtctgct gcctccataa ttttatttac ttcttcagcg gtaactttta
    40081 cctgtgactt gtcatccagc ggctgcgggt cctgatgatg ttcaactgta tccgctgcca
    40141 ttgtttcagc cgttgcctgt tcatctgcca ttgcgccaga tggttgcggg ttttcttcat
    40201 catttttttc ttcttctgtt ttgcactcag cagccagtcc gtcgttaatt tcttccagga
    40261 tatctttttc cggtgtatgc cgggcagcca tgagcgtttc agctgtggga ttctcgtgat
    40321 cagtttctgt caggttggca ttgatatacc cctgaaggcg tcccgggtag tgataaaact
    40381 cagggtgggc gctccggata agtgcaaaaa tagctgcgcg ggaatagtcc aggataccag
    40441 gggttgcgcg aagtgctgcg gaccattccc tgaacggact ttcttttttc aggacgattt
    40501 cttttgcgcg acgataaacg ctcacgggta tttcataaat attaaaatcc atcggaagcg
    40561 tggctgctgc agcctccaca tccagagtat cgagggtgtg tactaagtca ggattacgat
    40621 cggttttatt tccgccaccg gcatttgcac cggaagccgt gcgggtgatg cgagaaacac
    40681 gatttccttt catccactct tttgtcagca gacctcgatc ggtatagtca gcgtccaggt
    40741 atgcttcgaa aaaagcagtc attaacccca gactggaatt aacgggatta gggaaaactt
    40801 tgtctgtgtc gcgtaccagt ttatgaaggt cgcgaatctc cagcgggtcg agcagctttg
    40861 tgttgtggga aatagccagg gcagtaacag ccggcagttc ttcagctcgt gcgatatgta
    40921 atgcctgaag ttcttcccgt gaaacgtgcg ttaccggttt ttcgctgcca tgttgcgcaa
    40981 gccagcgaat gggtagttcc tgaccggaaa ttggcaggag catattatcc tcaatctcag
    41041 ccatgtcttc gccattgacg ttggtattgt cagtgctggc tggtttttcc tgaactgagg
    41101 gggaaggcgc tataaatatc attgtgatgc catcttcccc gcctttttca tagcggttgc
    41161 agaattcagt atcaaacacg ccttccggtg gaaggtcatt cacaacgggg aaatggacgc
    41221 ggacgggttt tttgaaatca tcagcgtcgt agcctgcctc gtccattgca acaacgcacc
    41281 gcgagattgc tacagataat ttcttttctg tactccagta aaaaccccct ttaatcccaa
    41341 agcgttttct gaccttgtca ttttttgctt cgcaatatag cgcaaattct tctttatcag
    41401 tgctcattga taaacctcat aactatttta aggttgtacg aacccctgcc attgctggca
    41461 tgcttaatca acgggtatgg cgttaatatg gctggtggat tatccagccg gtatttcgtt
    41521 attcaggtac agcgatactt tttttaacgg gaggcattcg ccagaaattt tttgctcgtc
    41581 tcttgcccgg aggcatgatt ctttactggc ataaattccg gtaatcacat tctgtggctc
    41641 acccgttata agaaaaacag tcattaccag tgcaaatgca gaagtcactg ctgccccccg
    41701 aaaataccaa gtttaagaag ggcaattctg gagagtatgg aattatcatt gagaagataa
    41761 ggctcatatt ttctcatctt aatggcatct tcagtaaact cccggttact gagcagaaca
    41821 ccaatatcaa aacacccttc agacgtatta acgtttggta ataacgtttc cattatcgtg
    41881 tcctcaacaa tgaattttgt gatgtggtgc ctggtgcctc caggtgacgt taaccagtta
    41941 acaattaacg ccggaataca gaaggatgcc cgttacgccc cgtaaaagac cactttacgg
    42001 atttaactgt tccgcgtgcg catagccgca ttcaccgcat cacaaaattc actttaaaaa
    42061 ggatggcaga gcagtcatgg agtcgaactg atgccaccaa agactacaca tggatattgt
    42121 ggcggggctg tcacttaagc gtatggtcag cctgacaacc cggtgtcctc aaggggaagg
    42181 aataaccccg ccatacttac cgccgcgcca tttcgcggat tgccacaacc ggaagcgcac
    42241 ggtcgaatta aatttaacga caaagtacag agagacgagc ttctccgtgc gctttcgtgt
    42301 tatgccctga cttttcaggg taaattaacc agtggcaacc tgtttttact ggtgctaatc
    42361 agttagtgtt tctggctaac cagcgacgcg cgccagcttc ggttttaaac tttttacttt
    42421 tggtatacgt catcgcggta aacgtgccgt cctggttagg gaacacaccg cataccagag
    42481 attcgttgtt gccaagattg agcgtatcca tgttgacctc atttcccctt aacgccgggg
    42541 tagcggaaca aaaaacctgc tgcatagtta aacgtaatcc ccgccgtcat gttcatacgc
    42601 ctcaggctgg ctacttaacc cctgaccact gccgggtaac tcgaagtatt gccctgcgtt
    42661 ctgtgggtcg gggtgggtgt atggatacaa tttacaaatt aaaatttaac tggtcaacat
    42721 gtttattatt aaatttgtaa ttgcgagctg ttgcattagt cccaaaatgg gactactata
    42781 cagttatgaa gattatctca gttaaaacac tcagggattt ttgggcggag aatcctgatg
    42841 cagaacaacc gctaagggca tgggtggatg aggcggcaaa agctgactgg aaaagcccag
    42901 cagacattaa ggcacaatac cgaacggcca gtatattgaa aaaccggcgc gtggtattca
    42961 acgtaaaagg taatcattac cgtttaattg ttgcaattgc gtatcagcga ggatgggtat
    43021 ttgttaaatt catcggaaac cacaaggaat acgatgctat tgatgctgat accatcgaac
    43081 tggagtaagc atgaacatca aacctattcg tacagagcaa gattatgaag ccgcgttgcg
    43141 tgctgttgaa cccatgttcg acaatgagcc cgaaattgac acgcctgagg gggatttttt
    43201 tgaggtgatg tgtttgctaa tagaggaata tgaaaaaaaa cattatccca ttgagccacc
    43261 atccccaatt gaagctataa gatttcgcat ggaacagcag gggctgactg tgaaagattt
    43321 ggaacccgca attgggaaaa aaaatcgggt ttatgaggtg ttgaatggta ccagaagcct
    43381 tacgttacca atgattcgcc gtcttcataa tcaatttggt atccccctgg aaagcctggt
    43441 tggattataa aatctgctag tcatttgcct gatgctcgtt ccagaaaagg aatgcatcag
    43501 gcagttttgt ttttctgccg cagtaactct tcaagtttcc gtttatagaa atcgcgtttt
    43561 tgctccatat cacgaatgat ctgctctgcg tcgctttgag gtaactcatc taaaagcgat
    43621 atgatttttc gttgttgttc tgtaagttgc ggttggttgt cattactgga tacagccatt
    43681 ttatcgccga gagtttcttc ttccataaag aaccaatgga cgggatgttg tgagagctct
    43741 gctaattttt ccagtttatc cattcttggc atcacgcctt tcaaccaacc ttgcacggat
    43801 tggggtttta caccaagacg tcttcccagc tctgactggt ttatattcaa ttcctgcaac
    43861 acctgctgaa ggcgttttac aaagatcatc accacccctc gtaaactagt tccgcgatcc
    43921 tacagaaaaa attgataagt ggcattacaa atagaagttg aaatttaaat ttaaatttgt
    43981 aatcatcggt gtcatcgtaa agttcggagg gaaacatgca aaaaagtact caagtgaaaa
    44041 tcctgtcaat aatgagccaa tcagaattag ggcgtcgtct tggtaaaaca ccgcaaacca
    44101 taagtgggtg gtttaaaaaa cgagtgccag cggaggaggt tataccagca tgtgaggcgc
    44161 ttgactgggg agtaactccg catgaattgc gccctgataa ataccctaat ccaaccgatg
    44221 gtttacctgt tgagtatcag gctaacgcac aagcagcggc gggagttgat tcatgaaaat
    44281 caagcatgag cacatccgca tggcgatgaa tgcgtggctg ctttatccga gggtagggcg
    44341 caaaaaaatc gctgatgata tagcgacagc atattttgag cttgaaatga cttatccacc
    44401 aatgcatgac acctctacga cagagggtat tggattgaat atacaaaata tttttcgctg
    44461 gcttgaaaag gatacgcctg atgctgttga aaaaattcag gcactaattc cggctatcct
    44521 gactgttctt ccgcgtgaac tgcgttatca cctcagtatt tttgacactg ttgagcgccg
    44581 tgcattactg gcggcgcagg aagcgttgag cacggcaatt gatgcacatg atgatgctgt
    44641 ccaggccgtt taccggaaag cgtatttcag cgacggcggg tcatccggcg agtctgttgt
    44701 ggtgcattga tatttatgcc agccccctgc tgattctgtt gattggggaa tcacagaata
    44761 tcaccagagg atggttcgtc acaagatgag gcaattatgg ccgcattacc atacatgcaa
    44821 ctgtacatag ctgattacct ggctgacacc atgcatttgt ccgcagagga gcacggtgcg
    44881 tatttgttgc tgatgttcaa ttactggcaa acagggaaac caatacctaa aaacaggctg
    44941 gcaaaaattt cccgtctgac taacgagcga tgggctgatg tggaaccatc cttgcgggag
    45001 tttttttgcg ataacggcga tgaatgggtg catcttcgga ttgaggaaga tctggcatca
    45061 gtcagggaaa aattgaccaa aaaatcagcc gccggaaaag catctgttca ggccagaaga
    45121 agcagaaagg aagtatatgt tcaaacaaaa caagagagag atttaacagg tgttcaaaca
    45181 aatgttggtg ttgtgtttga acatgacgca aacacaaaag caactaataa agatacagat
    45241 ctaaaagaat taaaccccac acataacgtg cgtgaacgcg agagtattcc gaccagtgag
    45301 ttgcatggtg cgccgttgca gacagccgaa cctgaatacc tggacggcct gagcgaaccg
    45361 atcgggaaat tttcgatgac cactgtctgg cagccgtcgc cggattttcg acaacgggca
    45421 gcagtgtggg gtatggctct gcctgagccg gaatttacac ctgctgagct tgccgcattc
    45481 cgggattact ggatggcgga ggggaaggtt ttcacacagg ttcagtggga gcagaaattt
    45541 gcccgccacg tgcagcacgt cagggcacag gtaaaaccag tcagcaaggg ggtaagccat
    45601 gcagcatcag gtggcacggc atcacgggca attcaggaaa tccgggcagc acgcgaacag
    45661 tgggaacgag acaacggatt tatcagcaac ggaaacggcc tggaagct
//
LOCUS       DAZGHF010000021        44909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGHF010000021 DAZGHF010000000
VERSION     DAZGHF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.021"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            102..1337
                     /gene="moeA"
                     /locus_tag="ACHK3A_001321"
     CDS             102..1337
                     /gene="moeA"
                     /locus_tag="ACHK3A_001321"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO8739434.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            1337..2086
                     /gene="moeB"
                     /locus_tag="ACHK3A_001322"
     CDS             1337..2086
                     /gene="moeB"
                     /locus_tag="ACHK3A_001322"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO8739435.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYHCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCGVCGQ"
     gene            complement(2162..2824)
                     /gene="fsa"
                     /locus_tag="ACHK3A_001323"
     CDS             complement(2162..2824)
                     /gene="fsa"
                     /locus_tag="ACHK3A_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO8739436.1"
                     /translation="MELYLDTSDVVAVKTLSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            2955..3854
                     /gene="ybiY"
                     /locus_tag="ACHK3A_001324"
     CDS             2955..3854
                     /gene="ybiY"
                     /locus_tag="ACHK3A_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO8739437.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            3860..6292
                     /gene="ybiW"
                     /locus_tag="ACHK3A_001325"
     CDS             3860..6292
                     /gene="ybiW"
                     /locus_tag="ACHK3A_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO8739438.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQAKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            6438..7253
                     /gene="ybiV"
                     /locus_tag="ACHK3A_001326"
     CDS             6438..7253
                     /gene="ybiV"
                     /locus_tag="ACHK3A_001326"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO8739439.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNIAPFNS"
     gene            7405..8664
                     /locus_tag="ACHK3A_001327"
     CDS             7405..8664
                     /locus_tag="ACHK3A_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO8739440.1"
                     /translation="MTFSCETLPTDAKAAIREMKAALREQLSDVQQVFDTLSAKIETR
                     VAEIDALKAQGLPVWPEVSYSDIAAGTVSDATRNEIKRRGCAVIKGHFPREQAMAWDR
                     AMLDYLDINHFDDVYKGPGDSFFGSLEASRPEIYPIYWSQAQMQARQSENMAAVQSFL
                     NRLWKSESNGKQWFNPDISVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQRV
                     FANVFNGNFDDYDPWDAAHRTDVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLLHV
                     VPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAGDS
                     VWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFPRE
                     DYEANWEGRFTLEDLNIHGKRALGMDV"
     gene            complement(8905..10497)
                     /gene="ybiT"
                     /locus_tag="ACHK3A_001328"
     CDS             complement(8905..10497)
                     /gene="ybiT"
                     /locus_tag="ACHK3A_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO8739441.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIIGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            10716..11636
                     /gene="ldtB"
                     /locus_tag="ACHK3A_001329"
     CDS             10716..11636
                     /gene="ldtB"
                     /locus_tag="ACHK3A_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO8739442.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(11694..12812)
                     /gene="ybiR"
                     /locus_tag="ACHK3A_001330"
     CDS             complement(11694..12812)
                     /gene="ybiR"
                     /locus_tag="ACHK3A_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO8739443.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGAQTPEWKPRLVWSCLGLYIVFLMALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(12809..13276)
                     /gene="mntR"
                     /locus_tag="ACHK3A_001331"
     CDS             complement(12809..13276)
                     /gene="mntR"
                     /locus_tag="ACHK3A_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO8739444.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVYMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            13462..13590
                     /gene="mntS"
                     /locus_tag="ACHK3A_001332"
     CDS             13462..13590
                     /gene="mntS"
                     /locus_tag="ACHK3A_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO8739445.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            13862..15445
                     /gene="opgE"
                     /locus_tag="ACHK3A_001333"
     CDS             13862..15445
                     /gene="opgE"
                     /locus_tag="ACHK3A_001333"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO8739446.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(15494..16009)
                     /gene="ompX"
                     /locus_tag="ACHK3A_001334"
     CDS             complement(15494..16009)
                     /gene="ompX"
                     /locus_tag="ACHK3A_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO8739447.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(16062..16127)
                     /locus_tag="ACHK3A_001335"
     CDS             complement(16062..16127)
                     /locus_tag="ACHK3A_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739448.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            16362..17249
                     /gene="rhtA"
                     /locus_tag="ACHK3A_001336"
     CDS             16362..17249
                     /gene="rhtA"
                     /locus_tag="ACHK3A_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO8739449.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            17549..18052
                     /gene="dps"
                     /locus_tag="ACHK3A_001337"
                     /gene_synonym="pexB"
     CDS             17549..18052
                     /gene="dps"
                     /locus_tag="ACHK3A_001337"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO8739450.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            18456..19202
                     /gene="glnH"
                     /locus_tag="ACHK3A_001338"
     CDS             18456..19202
                     /gene="glnH"
                     /locus_tag="ACHK3A_001338"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO8739451.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            19341..20000
                     /gene="glnP"
                     /locus_tag="ACHK3A_001339"
     CDS             19341..20000
                     /gene="glnP"
                     /locus_tag="ACHK3A_001339"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO8739452.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            19997..20719
                     /gene="glnQ"
                     /locus_tag="ACHK3A_001340"
     CDS             19997..20719
                     /gene="glnQ"
                     /locus_tag="ACHK3A_001340"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO8739453.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            20836..23061
                     /gene="ybiO"
                     /locus_tag="ACHK3A_001341"
     CDS             20836..23061
                     /gene="ybiO"
                     /locus_tag="ACHK3A_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO8739454.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTIWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(23058..23984)
                     /gene="rlmF"
                     /locus_tag="ACHK3A_001342"
     CDS             complement(23058..23984)
                     /gene="rlmF"
                     /locus_tag="ACHK3A_001342"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO8739455.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            24260..24520
                     /gene="mcbA"
                     /locus_tag="ACHK3A_001343"
     CDS             24260..24520
                     /gene="mcbA"
                     /locus_tag="ACHK3A_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO8739456.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            24785..27067
                     /gene="fiu"
                     /locus_tag="ACHK3A_001344"
     CDS             24785..27067
                     /gene="fiu"
                     /locus_tag="ACHK3A_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO8739457.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIKNEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            27109..27786
                     /gene="ybiX"
                     /locus_tag="ACHK3A_001345"
     CDS             27109..27786
                     /gene="ybiX"
                     /locus_tag="ACHK3A_001345"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO8739458.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            27860..28126
                     /gene="ybiI"
                     /locus_tag="ACHK3A_001346"
     CDS             27860..28126
                     /gene="ybiI"
                     /locus_tag="ACHK3A_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO8739459.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            28391..28651
                     /gene="ybiJ"
                     /locus_tag="ACHK3A_001347"
     CDS             28391..28651
                     /gene="ybiJ"
                     /locus_tag="ACHK3A_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO8739460.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(28791..29876)
                     /gene="hcxB"
                     /locus_tag="ACHK3A_001348"
     CDS             complement(28791..29876)
                     /gene="hcxB"
                     /locus_tag="ACHK3A_001348"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO8739461.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAVVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGMPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQKTLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARKVGMPEETLQAFCQQLAS"
     gene            complement(30017..30979)
                     /gene="ybiB"
                     /locus_tag="ACHK3A_001349"
     CDS             complement(30017..30979)
                     /gene="ybiB"
                     /locus_tag="ACHK3A_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO8739462.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIKRCLAGSEPIPESLKIQMACCLMATGEAATINDGLARINQAF"
     gene            complement(31007..33157)
                     /gene="dinG"
                     /locus_tag="ACHK3A_001350"
     CDS             complement(31007..33157)
                     /gene="dinG"
                     /locus_tag="ACHK3A_001350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO8739463.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            33261..33461
                     /locus_tag="ACHK3A_001351"
                     /pseudo
     CDS             33261..33461
                     /locus_tag="ACHK3A_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            complement(33696..35057)
                     /gene="rhlE"
                     /locus_tag="ACHK3A_001352"
     CDS             complement(33696..35057)
                     /gene="rhlE"
                     /locus_tag="ACHK3A_001352"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO8739464.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            35286..35957
                     /gene="cecR"
                     /locus_tag="ACHK3A_001353"
     CDS             35286..35957
                     /gene="cecR"
                     /locus_tag="ACHK3A_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO8739465.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            35960..36955
                     /gene="hlyD"
                     /locus_tag="ACHK3A_001354"
     CDS             35960..36955
                     /gene="hlyD"
                     /locus_tag="ACHK3A_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO8739466.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            36948..38684
                     /gene="ybhF"
                     /locus_tag="ACHK3A_001355"
     CDS             36948..38684
                     /gene="ybhF"
                     /locus_tag="ACHK3A_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO8739467.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            38677..39810
                     /gene="ybhS"
                     /locus_tag="ACHK3A_001356"
     CDS             38677..39810
                     /gene="ybhS"
                     /locus_tag="ACHK3A_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8739468.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            39821..40927
                     /gene="ybhR"
                     /locus_tag="ACHK3A_001357"
     CDS             39821..40927
                     /gene="ybhR"
                     /locus_tag="ACHK3A_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8739469.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(40889..41299)
                     /gene="ybhQ"
                     /locus_tag="ACHK3A_001358"
     CDS             complement(40889..41299)
                     /gene="ybhQ"
                     /locus_tag="ACHK3A_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO8739470.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            41432..42193
                     /gene="ybhP"
                     /locus_tag="ACHK3A_001359"
     CDS             41432..42193
                     /gene="ybhP"
                     /locus_tag="ACHK3A_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO8739471.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKMQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            42190..43431
                     /gene="clsB"
                     /locus_tag="ACHK3A_001360"
     CDS             42190..43431
                     /gene="clsB"
                     /locus_tag="ACHK3A_001360"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO8739472.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAMFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            43431..44387
                     /gene="ybhN"
                     /locus_tag="ACHK3A_001361"
     CDS             43431..44387
                     /gene="ybhN"
                     /locus_tag="ACHK3A_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO8739473.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(44423..44812)
                     /locus_tag="ACHK3A_001362"
     CDS             complement(44423..44812)
                     /locus_tag="ACHK3A_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739474.1"
                     /translation="MESYSQSSNKLDFQHEARILNGIWLITALGLVTTAGLALVIIRN
                     AALMSERPIWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVSCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
BASE COUNT        10733 a        11880 c        11515 g        10781 t
ORIGIN      
        1 gtaaaggcag gagccaacgc tacgcccgag gtggcataca gtctgtggcg tttttctgac
       61 ataataggca aattcgattt tgcctccgca ggagtgtttt catggaattt accaccggat
      121 tgatgtcgct cgacaccgcg cttaatgaga tgctttctcg cgttacccca ctgaccgccc
      181 aggaaacgct gccactggtt cagtgttttg gtcgtattct ggcgagcgat gtcgtttcgc
      241 cactggatgt cccggggttt gataactccg ctatggacgg ctacgcggtg cgtttagccg
      301 atattgcctc cgggcaaccg ctgcccgttg ccggtaaatc ctttgccggt cagccgtacc
      361 atggtgaatg gcctgcgggt acctgcattc gtattatgac cggtgcgccg gtgccagaag
      421 gctgcgaagc ggtggtgatg caggagcaga ctgaacaaac ggacaatggc gtgcgtttta
      481 ctgctgaagt gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc tctgcaggtg
      541 cggttgtttt cccggcgggg actcgcctga ctaccgctga actgccagtg attgcttcgc
      601 tagggattgc cgaagttccg gtgattcgta aagtgcgtgt agcgcttttt tctaccggtg
      661 atgaactcca gttgcccggt cagccgctgg gcgacggcca aatctacgat accaaccgtc
      721 tcgccgtaca cctgatgtta gaacagttgg gatgcgaggt aatcaactta gggattatcc
      781 gcgacgatcc ccatgccctg cgcgccgcat ttattgaagc cgacagccag gcggatgtag
      841 tgatcagttc cggcggtgtt tcagtgggtg aagcggatta caccaaaacg attcttgaag
      901 agctggggga gatcgccttc tggaaactgg cgattaaacc gggtaaaccg ttcgcgttcg
      961 gtaaactttc taatagttgg ttctgcggcc tgccgggcaa cccggtttca gcgacgctga
     1021 ccttctatca actggtacag cctttgctgg caaaactaag cggcaatacc gccagtggcc
     1081 tgcccgcgcg ccagcgtgta cgcacagcgt cccgcctgaa aaaaacgcca ggacgccttg
     1141 atttccagcg cggcgtgctg caacgcaacg ccgatggcga actggaagtg acgaccaccg
     1201 gacatcaggg ttcacatata tttagctcct ttagcctcgg caactgcttt atcgtgctgg
     1261 aacgcgatcg cggcaatgtg gaagtgggcg aatgggtgga agtagaaccg tttaacgcgt
     1321 tgttcggagg cctgtaatgg cggaactcag cgatcaggag atgctgcgct acaaccggca
     1381 aatcattctg cgcggttttg attttgacgg tcaggaagca ttgaaagatt cccgcgtgct
     1441 ggtcgtcggc cttggtgggc tcggctgcgc agcttcgcag tatctggcaa gcgccggtgt
     1501 cggcaacctg acgctgctcg acttcgacac ggtttcgctc tccaatctgc aacgccagac
     1561 gctgcacagt gatgccacgg tcgggcaacc aaaggtggaa tccgcccgtg acgccctgac
     1621 gcggatcaac ccacatatcg cgattacgcc agtcaatgcg ctgcttgatg acgctgaact
     1681 tgctgcgatg attgcaaaac acgatctggt gctcgactgt acggataacg ttgcagtacg
     1741 taatcaactg aacgcaggct gttttgccgc gaaggtgccg ctggtttccg gcgcggcaat
     1801 tcgtatggaa gggcaaatca cggtcttcac ttatcaggac ggcgaaccgt gctatcactg
     1861 ccttagccgt ttgtttggcg agaacgcgtt aacctgcgta gaagcaggcg tgatggcacc
     1921 gttaatcggc gtaattggtt cgttgcaggc aatggaagca atcaaactgc tggcaggtta
     1981 tggcaaacca gccagcggaa aaatcgtgat gtacgatgcg atgacctgtc agtttcgcga
     2041 aatgaaactg atgcgcaatc cggggtgtgg ggtgtgcggg cagtaactgc cacctccagg
     2101 ccagataaga cgcggcactc cgccacgtca tcagcaattt atgaggatgc agaacgtccg
     2161 gttaaatcga cgttctgcca aacgctccca gccagtcctg ttcaaacttc gccacagcgg
     2221 catcaaccgc cggatagcta atcatctgtt gtgccacatc cagcggcaga gtaattgatt
     2281 cacatcctgc cagtaagcag tccagcgcct gacgcggggt tttgaaactc gcggctaaca
     2341 ctttcgcctg cggcgcatgc attttcaata actggtgtaa gtcggtcaca gtctgaatac
     2401 cgctaccgcc ctgagcatca atacgattaa cgtaaggcgc aacatattcc gcacctgcca
     2461 gcgccgacag tagcccttgt gctgcgccat ataccgcggt tcccagcgtc ggaatccctt
     2521 ccgcttttaa catcttaata gctgccagcc cctcagcggt caccggaacc ttcaccacga
     2581 tatccgcaat aatagaacgc aatttacgtg cgtcattaac catcccttca gcagtggtag
     2641 ccattacctg ggcaaacaga cgcccctgac cgcccatcgc ctcgtgaagt tgcggaagca
     2701 caacctccag cggtttttta cccgctgcga taatgcttgg gttagtggtc acacccgcca
     2761 gcggaaaaat acgtgacagc gtcttcaccg caacaacgtc tgaagtatcc agatacagtt
     2821 ccataaccat cctcaaaatg tactttttgt ctgaatgtag actatcacgg gaaaactctc
     2881 tgaacagttg acgcacatca atctaacttt cattcgaaag tattttaatg tttgaacgaa
     2941 acagagaggc aactatgatt ttcaatattc aacgctactc gacccatgat ggccccggta
     3001 tccgcacggt cgtatttctt aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg
     3061 aaagccgcgc ccgcacgcag gatctgctgt atgacccacg actctgtctg gaaggctgtg
     3121 agctgtgcgc taagaccgca ccggaagtga ttgaacgcgc gttgaatggt ttgcttattc
     3181 atcgggaaaa gttaaccccg gagcatttta cggcgctaac cgactgctgc ccgacacagg
     3241 cgttaaccgt gtgtggtgaa gtgaaaagcg ttgaggagat catggcaacc gttttgcgcg
     3301 ataaaccgtt ttacgatcgc agcggcggcg gcttaacgct ttctggcggt gaaccgttta
     3361 tgcagccgga aatggcgacg gcgctgctgc aagccagcca cgaggccggc attcatactg
     3421 cggtagaaac ctgtctgcat gtgccgtgga aatatatcgc cccttctctg ccctatatcg
     3481 atctgtttct tgccgattta aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg
     3541 gtaacgccgc cagggtgctg gataacctga aaaaactcgc cgcagcgggc aaaaaaatca
     3601 ttatccgcgt gccactgatt cagggcttta atgccgacga aacctctgtg aaagccatta
     3661 ccgattttgc cgccgacgag ctgcacgtta gcgaaattca tttcctgccc taccacacgc
     3721 tgggcatcaa caaatatcac ttacttaatc tgccctatga cgccccggaa aaaccgcttg
     3781 atgcgccaga actgctcgac tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga
     3841 ccttacgagg ataacaatca tgacaacact aaaactggat acgctcagcg accgcattaa
     3901 agcgcacaaa aatgcgctgg tgcatattgt gaaaccgccg gtctgtaccg aacgagcaca
     3961 acactatacc gagatgtatc aacaacatct cgataagcca atcccggtac gtcgcgcgct
     4021 ggcactggcg catcacctgg cgaatcgcac catctggatc aaacatgatg aattgatcat
     4081 tggcaaccag gcaagcgaag ttcgcgccgc gccgatcttc ccggaatata ccgtctcctg
     4141 gatcgaaaaa gagattgatg atctggcaga tcgtcccggt gctggttttg cggtgagcga
     4201 agagaacaaa cgcgttctgc atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga
     4261 tcgctgctac ggcatgttta ccgatgagca aaaaggtctg ctggcgaccg gcatcattaa
     4321 agcggagggc aatatgacct ccggcgatgc gcacctggcg gtcaatttcc cgctactgct
     4381 ggaaaaaggt cttgatggtc tgcgtgagaa agtggcggaa cgtcgctcgc gcatcaatct
     4441 gacggttctg gaagatctgc acggcgagca attcctgaaa gcgattgata tcgtgctggt
     4501 ggcagtcagt gaacacattg aacgtttcgc tgccctggcg cgtgaaatgg ccgcaaccga
     4561 aacccgcgaa agccgtcgcg atgaactgct gacgatagca gaaaactgcg atcttatcgc
     4621 ccaccagccg ccgcagactt tctggcaggc gctgcaactg tgctacttca tccagttgat
     4681 tttgcagatc gaatctaacg gtcactcggt atcgtttggt cgtatggacc agtatctcta
     4741 cccatactat cgccgtgacg ttgaactgaa ccagacactg gatcgcgaac acgccatcga
     4801 gatgctgcat agctgctggc tgaaactgct ggaagtgaac aaaatccgct ccggctcaca
     4861 ctcaaaagcc tctgcgggaa gtccgctgta tcagaacgtc accattggcg ggcaaaatct
     4921 ggttgatggt caaccgatgg acgcggtaaa tccactctct tatgcgatcc tcgaatcttg
     4981 cggtcgcctg cgttcgactc agcctaacct cagcgtgcgt taccatgcgg gtatgagtaa
     5041 cgatttcctc gacgcctgtg ttcaggtgat ccgctgcggc ttcgggatgc cggcgttcaa
     5101 caacgacgaa atcgtgatcc cggaatttat taaactcggt attgaaccgc aggacgctta
     5161 cgactacgca gcaattggtt gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg
     5221 taccggcatg agctttatca acttcgcccg cgtgatgctg gcggcgctgg aaggtggtcg
     5281 tgatgccacc agcggcaaag tgttcctgcc acaagcaaaa gcgttgtcag cgggtaactt
     5341 caacaacttc gatgaagtaa tggacgcatg ggatacgcaa atccgttact acacgcgtaa
     5401 atcaatcgaa atcgaatatg tcgtcgatac catgctggaa gagaacgtac acgatattct
     5461 ctgctcggcg ctggtcgatg actgtattga gcgagcgaaa agtatcaagc aaggcggcgc
     5521 gaaatatgac tgggtttctg gcctgcaggt cggcattgct aacctcggca acagcctggc
     5581 ggcagtgaag aaactggtat ttgaacaggg cgcgattggt cagcaacagc tggctgccgc
     5641 actggcggat gacttcgacg gcctgactca cgagcagctg cgtcagcggc tgattaacgg
     5701 tgcgccgaag tacggcaacg acgatgatac tgtcgatacg ctgctggctc gcgcttatca
     5761 gacctatatc gacgaactga agcagtacca taacccgcgc tacggtcgtg gtccgattgg
     5821 cggcaactat tatgcgggca catcgtctat ctccgctaac gtaccgtttg gcgcgcagac
     5881 gatggcaacg ccggacggac gtaaagcaca taccccgctg gcagaaggcg ctagtccggc
     5941 ctccggtact gaccatctcg gccctactgc ggtcattggc tcggtgggta aactgcctac
     6001 ggcagcaatt ctcggcggcg tgctgctcaa ccagaaactg aatccggcaa cgctggagaa
     6061 tgaatctgac aagcagaaac tgatgatcct gctgcgtacc ttctttgaag tgcataaagg
     6121 ctggcatatt cagtacaaca ttgtttcccg cgaaacattg ctggaggcga agaaacatcc
     6181 ggatcaatac cgcgatctgg tggtacgcgt agcgggttat tccgccttct tcaccgcgct
     6241 ctctccagac gcacaggacg atatcatcgc ccgtaccgaa cacatgctgt aatcccctca
     6301 gcccggcgtc gtcgtcgccg ggccaaatca ttcacatcat caatttcagc ctcgctttca
     6361 ttcgaaatat aatttgtgct ctgcgtcaca ttgttattac actgttacgg tttcattttc
     6421 gagccaggag cacacctatg agcgtaaaag ttatcgtcac agacatggac ggtacttttc
     6481 ttaacgacgc caaaacgtac aaccaaccac gttttatggc gcaatatcag gaactgaaaa
     6541 agcgcggcat tgagttcgtt gtcgccagcg gtaatcagta ttaccagctt atttcgttct
     6601 ttcctgagct aaaggatgag atctcttttg tcgcggaaaa cggcgcactg gtttacgaac
     6661 atggcaagca gttgttccac ggcgaactga cccgacatga atcgcggatt gttattggcg
     6721 agttgctaaa agataagcaa ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca
     6781 gcgaaaatgc ccccgaagca ttcgtcgcac tgatggcaaa acactaccat cgcctgaaac
     6841 ctgtaaaaga ttatcaggag attgacgacg tactgttcaa gttttcgctc aacctgccgg
     6901 atgaacaaat cccgttagtg atcgacaaac tgcacatagc gctggatggc attatgaaac
     6961 ccgtcaccag tggttttggc tttatcgact tgattattcc cggtctacat aaagcaaacg
     7021 gtatttcgcg gttactgaaa cgctgggatc tgtcaccgca aaatgtggta gcgattggcg
     7081 acagcggtaa cgatgcggag atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg
     7141 ctgcggaaaa cattaaacaa atcgcccgtt acgctaccga tgataataat catgaaggcg
     7201 cgctgaatgt gattcaggcg gtgctggata atatcgctcc tttcaatagt tgatcttccc
     7261 cctggccgaa gtgaccactt cggcctctct cctttttgct cgctttgtgc actgactcaa
     7321 tatttttatc ttgcgttaac ttatttttaa cttaatgtat caatcgcaat cattttactt
     7381 tcgaatgaaa gaagcgaggt tgatatgact ttttcctgtg agacgctgcc cacagatgcg
     7441 aaagcagcta tccgagagat gaaagctgcg ctgcgggaac agctcagtga tgtgcaacag
     7501 gttttcgata cactctctgc aaaaatcgaa acgcgtgttg ctgaaatcga tgccctaaaa
     7561 gcgcaagggc tgccggtttg gccggaagtt tcttatagtg atattgccgc aggtacagta
     7621 agcgatgcca ctcgcaatga aattaaacgt cgcggttgtg cagtcataaa aggccatttc
     7681 cctcgcgagc aggcgatggc gtgggatcgt gccatgctcg attatcttga tatcaatcat
     7741 tttgatgatg tttataaagg ccctggcgac agttttttcg gctcgctgga ggcttctcgt
     7801 cccgaaatct atcccattta ctggtcgcaa gcacagatgc aggcgcggca gagcgaaaac
     7861 atggctgctg tgcaatcttt tctaaaccga ctgtggaagt ctgaaagcaa cggcaaacag
     7921 tggtttaatc ctgatatcag tgtgatttat ccagacagga tccgccgccg ccctccaggt
     7981 actacatcta aaggcctggg cgcgcatacc gattctggtg cgctggaacg gtggttatta
     8041 cccgcttatc agcgcgtttt tgccaatgtg tttaatggta atttcgatga ttacgatccc
     8101 tgggatgctg cacaccggac cgatgtcgaa gagtacacgg tagataacac tacaaaatgt
     8161 tcggtgttcc gcacgtttca gggctggacg gcgctctctg atatgttatc gggtcaggga
     8221 ttgttacatg tggtgcctat cccggaagcg atggcgtatg ttttattgcg cccgctgctg
     8281 gatgacgtac cggacgatga actgtgcggg gttgcacctg gcaaagttct gcccatttca
     8341 gagaaatggc atccactact gcttaaagcg ctgacttcca ttcccgcgat aacagcaggg
     8401 gattccgtct ggtggcactg cgatgtgatt cactccgtcg cgccagtaga caatcaacag
     8461 ggttggggca acgtgatgta tatccctgct gcgccaatgt gcgagaaaaa tcgggcttat
     8521 gcacacaaag tcaaaatggc gctggaaaaa ggcgcatcac ctggcgactt cccgcgggaa
     8581 gattatgaag caaactggga aggccgcttt acgctggagg atctgaacat tcacggtaag
     8641 cgcgcactgg gcatggatgt ttgagagtta atggaagtga attgatgtgg gattaagaaa
     8701 atcgattcaa cgttactcag attgctgaca aatgcgcttt gttaatgcct gatgcggcgt
     8761 taacgcctta tccggcctac aaagtcctgc agattcaata tattgcaggt actgcgtagg
     8821 cctgataagc atagcgcatc aggcaatctt gcgtttgtca tcagccgccg tcaccccgac
     8881 agtatcgggc tgacggataa taacttactc gatcccttta ctacgcagat aatcttcgta
     8941 attaccgcta aagtcgatca cgcgttccgg ggtgatttcc agaattcggg tcgccaggga
     9001 gcttacaaac tcacggtcgt gagacacaaa gatcagcgtg ccctgataca gttccagcgc
     9061 catgttcagc gactcaatgg attccatatc caggtggttg gtcggttcgt ccatgatcag
     9121 aatgttcggc ttctgcatca ttaacttacc aaacagcatc cgccctttct ccccaccgga
     9181 aagcactttc gctggctttt tgatgtcgtc ctggctgaac agcaaacgac cgagaatgct
     9241 gcgtaccgcc tgctcgtcat cgccttcctg cttccactga ctcatccatt cgaacacggt
     9301 aagatcattt tcgaactcat attcgtggtc ctgggcgtag taaccaatgc gcgcgttttc
     9361 agaccatttt acggtgccgc tgtccggttg cagatcgccc accagcgttt tcaacagtgt
     9421 tgatttaccg acgccgttgg tacccagtac cgccagtttt tcaccgactt ccagcagcag
     9481 gttgagattt ttaaacagcg gaccgttatc aaaccctttg gtcagacctt ccacttccag
     9541 cgcgttacgg aacagtttct tatcctgttc aaaacggatg aacgggttct gacggctgga
     9601 ggctttcacc tcttccagtt tgattttatc aatctggcgc gcgcgcgaag ttgcctggcg
     9661 agatttcgag gcgttggcac taaagcggct aacgaaagat tgcagctcag caatctgcgc
     9721 tttcttcttg gcgttatcag ccagcagacg ctcacgcgcc tgggtcgccg ccgtcatgta
     9781 ctcatcgtag ttacctggat aaacgcgcag ctcgccgtaa tccagatccg ccatgtgggt
     9841 acaaaccatg ttaaggaagt gacggtcgtg cgagatgatg atcatggtgc tgtcacgctc
     9901 gttcagcacc tgttccagcc aacgaatggt gtcgatgtcg aggttgttgg tcggttcgtc
     9961 gagcaggaga atgtccggat cagcaaacag cgcctgcgcc agcagcacac gcagcttcca
    10021 gccaggagca acttcactca tcgggccgta gtgctgttcc actggaattc ccacgccaag
    10081 caacagttca ccggcgcgag cttccgcaga gtaaccgtcc atttcgccgt atttcacttc
    10141 cagatcggcc actttatagc cgtcttcttc actcatttcc ggcaaagcat agatgcggtc
    10201 acgctcctgc ttcacttccc acaactcttt atgcccaatg atcaccgtat ccagcacagt
    10261 gaactcttca aaggcaaact gatcctgacg cagcttacca atgcgctcgt tgggatcgag
    10321 ggaaacgtta cccaacgtcg gctctaagtc gccgccgagg atcttcataa aggtggattt
    10381 accactaccg ttcgcgccaa tcaggccgta acggttgccg ccgccaaatt tgacggaaat
    10441 gttttcaaac aacggcttac tgccgaactg catggtgacg ttactggaaa ctaacacagg
    10501 cgtatcctga aaagagatat gacaaaccgc gcattatgcc ataaatctga aagaagatcc
    10561 tgctcctcgc ttaaggtcta cactcaagcc aaaggcaaaa aatgtgattt cgtacacatc
    10621 tgatttcact gtgagctgga atgaacttat aatgcgcttc caatactcta atattctcaa
    10681 cccaatggcc tgccaggcac aaaatctcgc ttaacatgaa tatgaaattg aaaacattat
    10741 tcgcagcggc cttcgctgtt gtcggctttt gcagtaccgc ctctgcggta acttatcctc
    10801 tgccaaccga cgggagtcgc ctggttggtc agaatcaggt gatcaccatt cctgaaggta
    10861 acactcagcc gctggagtat tttgccgccg agtaccagat gggactttcc aatatgatgg
    10921 aagcgaaccc gggtgtggat accttcctgc cgaaaggcgg tactgtactg aacattccgc
    10981 agcagctgat cctgccggat accgttcatg aaggcatcgt cattaacagt gcagagatgc
    11041 gtctgtatta ctatccgaaa ggaaccaaca ccgttatcgt gctgccgatc ggcattggtc
    11101 agttaggcaa agacacgcct atcaactgga ccaccaaagt tgagcgtaaa aaagcaggcc
    11161 cgacctggac gccgaccgcc aaaatgcacg cagagtaccg cgctgcgggc gaaccgcttc
    11221 cggctgtcgt tccggcaggt ccggataacc cgatgggtct gtatgcactc tacatcggtc
    11281 gcctgtacgc tatccatggc accaacgcca acttcggtat cggcctgcgt gtaagtcatg
    11341 gctgtgtgcg tctgcgtaac gaagacatta aattcctgtt cgagaaagtg cctgtcggta
    11401 ctcgcgtgca gtttattgat gagccggtga aagcgactac cgaaccagac ggcagccgtt
    11461 acattgaagt ccataacccg ctgtctacca ccgaagccca gtttgaaggt caggaaattg
    11521 tgccaattac cctgacgaag agcgtgcaga cagtgaccgg tcagccagat gttgaccagg
    11581 ttgttcttga tgaagcgatc aaaaatcgtt ccgggatgcc ggttcgtctg aattaatcgt
    11641 caaaacgtaa aacaaaggcg gactcataat ccgccttttt tatttgccag accctaccag
    11701 gccgggatca taaccaacaa aacatatccc accagcgccg cccacaacag catcggtatc
    11761 gaataaagat gaaaacgcca ccagatacgg cgatcgttcg ccatccgtaa cgcaatcaaa
    11821 tttgccagcg atccgggtaa taaaccaaag ccccctacgt ttaccgccca tgccagcagc
    11881 aacgacggcg gcacatagtt cagcaacaat atggtactcg gcacgttact gatcacctgc
    11941 gataaaccga ttgccgttaa ccataatccg ggttcggata aatgactcac gttacttaac
    12001 accccctgca aagctggcag ttgggttagt aaatggacgt cgataaacat cgccataaac
    12061 accagcagca gcgtccagtc cacactgagc accacgcgac gtgccagcag cgcaaagccc
    12121 accgccacaa tcaccagtcc ccacagctct tgttttaact ccagcgccat cagaaagacg
    12181 atatacagcc ccagacaact ccacaccagc cgcggtttcc actccggcgt ttgcgccccc
    12241 gtatggtatt gcagcgcctt tccagggaaa caacaccagc acaacagcag gagtgtcagc
    12301 atcattgcgc cagccagcgg tgccatttgg gcaataaatc cggcaaacga aagaccagaa
    12361 cgtccccaga taagaatatt ttgcgggttg ccaattggcg tcagtagcga accagcgttg
    12421 actgccagcg cctcaaaaat aatcagccga ttaaccggga tctcacacag tctttttagc
    12481 gtgatagtca gcggaacaac aataaacagc gcgacatcgt tggtcagaaa ggtagaaagc
    12541 agcgccgccg ccagcaccat aaacatcgcc agccgacgct ccgtagcaaa gcggcgcacc
    12601 attttgcgcc ccagcacatc aaaataaccg cttaactcca cacctttggt cagcagcatc
    12661 aggccgctta aggtgatgat ggtgtgccag tcgatagcag caggccagga tttcggtgca
    12721 aagggaacga aaaaacttaa tccgatgcca acaagaatta ataactgaaa aaaacgatcg
    12781 ccttgcagcg tgcgtaaaaa aggcaggctc atttggcacc gtgtttctgg gtaaacaaac
    12841 gaaaggcatc cagcgtctct tcactaacat ggtgctccat gccttccgcg tcgcgacggg
    12901 cgatttccgg actgacgccc aacaccaaca agaaattttc gactatctga tgtcgctcgc
    12961 ggctttcctg cgccagtttc tctccttctg ccgttaaaaa cacgccacgc caggggatca
    13021 tttcaatcag ccccatggtt gccagtcgct taagcatttt agccaccgtc ggttgcgaaa
    13081 ctcccaaacg agcagccatg tatacctgac gagcttcccc cacttccctg atcaagtcag
    13141 aaatcagctc aacgtaatca tcaatcagct cgcgccgatg cgcctctctg acctggcgga
    13201 acccttcaac gtgctcttcc acgttcacta actgcgtcac tttttttgct gttggcgtac
    13261 ctgcgcgacg actcattgtg cttcctcatt atggtgacgc atacatagcg ttctcatttc
    13321 gtgcgcacat tgtaaaccag agttgcgaag gtacaaaaaa ttaacgtttt agcaatagct
    13381 atataatata gcctgtgcta tatctgtatg taatgcaatc atccctcaag gatcgacggg
    13441 attagcaagt caggaggtct tatgaatgag ttcaagaggt gtatgcgcgt gtttagtcat
    13501 tctcccttta aagtacggtt aatgctgctc tctatgttgt gcgatatggt caacaacaaa
    13561 ccgcagcaag ataaaccttc agataaatag cggcgtcgcg gtacgccgct tcactcctgc
    13621 ttttatgcag gcataacgcg ttttggtctg aaaaacccca ctttttgtcg gatttgcaat
    13681 ccccttcgca aaagatttgt tcgtcagtag ttgacctgaa cggcggctcg ctctatcttc
    13741 ttgcagccct gcgtatattg cggctcgcgg atgcggaccc ctttcccctc tccacgcact
    13801 cttgcaggta ttgacccttg acgccaaggt aagcacatgg cgtttgttac gatagtggca
    13861 tatgaattta accctcaaag aatcgcttgt tacccgtagc cgggtattta gcccgtggac
    13921 tgcgttctac tttttacagt cgctattaat taacctcggc ttaggttatc ccttcagttt
    13981 gctctacacc gctgcgttta cggctatttt gcttttgcta tggcgaacat tgcctcgcgt
    14041 acaaaaagtt ctggtcggtg tcagttcgct ggtggcggct tgttatttcc cttttgctca
    14101 ggcctacggc gcacctaatt tcaatacatt gctggcattg cactccacca atatggaaga
    14161 gtcgaccgaa atcctgacga tttttccgtg gtacagctac ctggtcggct tatttatttt
    14221 tgcgctcggc gtaatagcaa tcaggcggaa aaaagagagt gaaaaagcgc gctggaatac
    14281 cttcgacagc ctgtgtctgg tattcagtgt ggcgacattt tttgttgctc ccgtgcaaaa
    14341 cctggcctgg ggtggcgtat ttaaactgaa agatactggc tatccggtat ttcgttttgc
    14401 taaggatgtc atcgtcaata ataacgaggt gattgaagag caagaacgga tggcaaaact
    14461 gtccggaatg aaagatacct ggacggtcac tgccgttaag ccgaagtatc agacctatgt
    14521 ggtggtgatc ggagaaagcg cgcgtcgcga tgccctcggt gcctttggcg gtcactggga
    14581 caataccccg tttgccagca gcgttaacgg tttgatattt gctgactaca ttgccgccag
    14641 tggctccacg cagaaatcgc ttggcttaac gctcaatcgc gttgtcgatg gcaaaccaca
    14701 gtttcaggat aactttgtca ccctggcaaa tcgcgcgggc ttccagacct ggtggttttc
    14761 caaccagggc caaatcggcg aatacgatac cgccatcgcc agcatcgcca aacgtgcaga
    14821 tgaagtgtac ttcctgaaag aaggtaattt tgaagcagat aaaaacacga aagacgaagc
    14881 gttactggat atgaccgctc aagtgctggc gcaagagcac tcgcaaccgc agctgattgt
    14941 tttacatctg atgggctcac atccgcaggc ctgcgaccgg acacaaggca aatacgaaac
    15001 ctttgtgcaa tcgaaagaaa cgtcgtgcta tctctatacc atgacgcaaa cagacgattt
    15061 actgcgtaag ctgtacgatc agttacgcaa cagcggcagt agcttctcgc tggtttactt
    15121 ttctgaccac ggtctggcct ttaaagagcg tggcaaagac gtgcaatacc ttgcccatga
    15181 tgataagtat cagcaaaatt tccaggtgcc ttttatggtc atttcgagcg acgataaagc
    15241 gcatcgggtg attaaagccc gccgctctgc caatgacttc ttaggctttt tctcccagtg
    15301 gacgggaatt aaagcgaagg aaataaatat caaatacccg tttatatctg agaagaaagc
    15361 cgggccgata tacatcacca acttccagtt acagaaggta gattacaacc atctcggaac
    15421 cgatattttc gacccgaaac cttaaaaaca aaaatccgcc ccgagaggcg gattttttat
    15481 atcaccgaag tgattagaag cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc
    15541 tacgaatacg gctctgctcg taagagaagt ccagagcaac gttttccatc gggttgaact
    15601 gcagaccagc accgtaggag aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt
    15661 cagtggtctg gaatttacca taacccacac ccactacacc gtagatgctt gcccagtcgt
    15721 taatgcggta agccggacca gcagtgatgc cgtagtactg gtttttgttg tagtcaccag
    15781 agcttgcagt acggcttttc tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt
    15841 tgtcttcttc atagcggtat ttcaggttga aaccgcccat tttgttcatt tggccctgag
    15901 cgtcgctctg tgcgtaaccg ccagttacgg tagaagtcgc agctacggaa gtacctgcgg
    15961 tgaaagccag aactgcggcc agtgctgaaa gacatgcaat ttttttcata accacctcaa
    16021 atgtgattca aataagtcct aagttttaaa tatatcaaaa attaatggga aactcttcgc
    16081 gatttgtgat gtctaacggg ccatttcatg taacagaacg tttccataca ccgctatcca
    16141 tctaaattta aatcactttt tcagagagct gcataagtat tacgcatgtt ttccctgtca
    16201 ttcatccaga ttattcctaa tcaccagact aatgattcca tcaatcctgg cgcattttag
    16261 tcaaaacggg ggaaaatttt ttcaacaaat gctcaaccag cattgggtat atccagtaca
    16321 ctccacgctt tacttaggtt tagatatttg tgggagaaag gatgcctggt tcattacgta
    16381 aaatgccggt ctggttacca atagtcatat tgctcgttgc catggcgtct attcagggtg
    16441 gagcctcgtt agctaagtca ctttttcctc tggtgggcgc accgggtgtc actgcgctgc
    16501 gtctggcatt aggaacgctg atcctcatcg cgttctttaa gccatggcga ctgcgctttg
    16561 ccaaagagca gcggttaccg ctgttgtttt acggcgtttc gctggggggg atgaattatc
    16621 ttttttatct ttctattcag acggtaccgc tgggtattgc ggtggcgctg gagttcaccg
    16681 gaccactggc ggtggcgctg ttctcttctc gtcgcccggt agatttcgtc tgggttgttc
    16741 tggcggttct tggtctgtgg ttcctgctac cgctggggca agacgtttcc catgtcgatt
    16801 taaccggctg tgcgctggca ctgggagcag gggcttgttg ggctatttac attttaagtg
    16861 ggcaacgcgc aggagcggaa catggccctg cgacggtggc aattggttcg ttgattgcag
    16921 cgttaatttt cgtgccaatt ggagcgcttc aggctggtga agcactctgg cactggtcgg
    16981 ttattccatt gggtctggcg gtcgctattc tctcgaccgc tctgccttat tcgctggaaa
    17041 tgattgccct cacccgtttg ccaacgcgga catttggtac gctgatgagc atggaaccgg
    17101 cactggctgc cgtttccggg atgattttcc tcggagaaac actgacaccc atacagctgc
    17161 tggcgctcgg cgcaattatc gccgcttcaa tgggttctac gctgacagta cgcaaagaga
    17221 gcaaaataaa agaattagac attaattaaa tttacatttc tgcatggtta tgcataacca
    17281 tgcagaattt atcgctactt ttcctctaca ccgtctttat atatcgaatt atgcaaaagc
    17341 atatttattc cgaaaattcc tggcgagcaa ataaataaga attgttctta ttaatatatc
    17401 taactcattg agtttttatt ggttttgttt tttcacgctt gttaccacta ttagtgtgat
    17461 aggaacagcc agaatagcgg aacacatagc tggtgctata cttagtctcg ttaattactg
    17521 ggacataaca tcaagaggat atgaaattat gagtaccgct aaattagtta aatcaaaagc
    17581 gaccaatctg ctttataccc gcaacgatgt ctctgatagc gagaaaaaag caacagtaga
    17641 gttgctgaat cgccaggtta tccagtttat tgatctttct ttgattacca aacaagcgca
    17701 ctggaacatg cgcggcgcta acttcattgc cgtacatgaa atgctggatg gcttccgcac
    17761 tgcactgatc gatcatctgg ataccatggc agaacgtgca gtgcagctgg gcggtgtagc
    17821 tctggggacc actcaagtta ttaacagcaa aactccgctg aaaagttacc cgctggacat
    17881 ccacaacgtt caggatcacc tgaaagagct agctgaccgt tacgcaatcg tcgctaatga
    17941 cgtacgcaaa gcgattggcg aagcgaaaga tgacgacacc gcagatatcc tgaccgccgc
    18001 gtctcgcgac ctggataaat tcctgtggtt tatcgaatct aacatcgaat aaatccatcg
    18061 ctgatggtgc agaactttag tacacgataa aggcggcttc ctgacaggag gccgttttgt
    18121 tttgtagccc gcctcaacgc acttatttag tgcatccatc tgctatcttc agctgattaa
    18181 gtaaattttt tgtatccaca tcatcacaca atcgttacat aaagattgtt ttttcatcag
    18241 gttttacgct aaataatcac tgtgttgagt gcacaatttt agcgcaccag tttggtgccc
    18301 cagaatggtg catcttcagg gtattgccct ataaatcgtg catcacgttt ttgccgcatc
    18361 tcgaaaaatc aatgagttgc aaaactggca cgattttttc atatatgtga atgtcacgca
    18421 ggggatcgtc ccgtggatag aaaaaaggaa atgctatgaa gtctgtatta aaagtttcac
    18481 tggctgcact gaccctggct tttgcggttt cttctcatgc cgcggataaa aaattagttg
    18541 tcgcgacgga taccgccttc gttccgtttg aatttaaaca gggcgataaa tacgtgggct
    18601 ttgacgttga tctgtgggct gccatcgcta aagaactgaa gctggattac gaactgaagc
    18661 cgatggattt cagtgggatc attccggcac tgcaaaccaa aaacgtcgat ctggcgctgg
    18721 cgggcattac catcaccgac gagcgtaaaa aagcgatcga tttctctgac ggctactaca
    18781 aaagcggcct gttagtgatg gtgaaagcca acaataacga tgtgaaaagc gtgaaagatc
    18841 tcgacgggaa agtggttgct gtgaagagcg gcactggctc cgttgattac gcgaaagcaa
    18901 acatcaaaac taaagatctg cgtcagttcc cgaacatcga taacgcctat atggaactgg
    18961 gcaccaaccg cgcagacgcc gttctgcacg atacgccaaa cattctgtac ttcatcaaaa
    19021 ccgccggtaa cggtcagttc aaagcggtag gtgactctct ggaagcacag caatacggta
    19081 ttgcgttccc gaaaggtagc gacgagctgc gtgataaagt caacggcgcg ttgaaaaccc
    19141 tgcgcgagaa cggaacttac aacgaaatct acaaaaaatg gttcggtact gaaccgaaat
    19201 aataacgcta cgcctgtaag gcgcactggc agttccctct cccctctggg gagaggatta
    19261 gggtgagggg cgcaaacccg ctccggggcc attaattacc ctgaatttga ttatttacac
    19321 cacggtaaca ggaacaacat atgcagtttg actggagtgc catctggcct gccattccgc
    19381 ttctgattga aggtgccaaa atgaccctgt ggatctcggt cctcggtctg gctggcggcc
    19441 tggtgatcgg attgctggca ggttttgcac gcaccttcgg tggttggata gccaaccacg
    19501 tcgcgctggt ctttattgaa gtgatccgcg gcacacctat cgtcgtccag gtgatgttta
    19561 tctatttcgc cctgccgatg gcatttaacg acttacgcat cgacccattt actgcggcgg
    19621 tggtcaccat catgatcaac tccggcgcat acattgcgga aatcacgcgt ggtgcggtgc
    19681 tgtctattca caaaggtttt cgtgaagcag gactggcgct cggtctttca cgttgggaaa
    19741 ccattcgcta cgtcatttta ccgctggcac tgcgtcgtat gctgccgccg ctgggtaacc
    19801 agtggatcat cagcattaaa gacacctcgc tgtttattgt tatcggcgtg gcggaactga
    19861 cccgtcaggg gcaagaaatt attgccggta acttccgcgc ccttgagatc tggagcgccg
    19921 tggcggtgtt ctatctgatt attaccctgg tgctgagctt tattctgcgt cgtctggaaa
    19981 gaaggatgaa aatcctgtga ttgaatttaa aaacgtctcc aagcattttg gtccaaccca
    20041 ggtgctgcac aatatcgatt tgaacattgc tcagggcgaa gtcgtggtga ttatcgggcc
    20101 gtccggttcc ggtaaatcga ccctgctgcg ctgcatcaac aaactggaag aaatcacttc
    20161 cggcgatctg attgtcgatg gcctgaaggt taacgatccg aaagttgacg agcgcctgat
    20221 tcgccaggaa gcaggtatgg tgttccagca gttttacctc ttcccgcatc tgacggcgct
    20281 ggaaaacgtc atgtttggtc cgctacgcgt gcgtggcgcg aacaaagaag aggcggaaaa
    20341 actggcgcgt gagctgctgg cgaaagtcgg tctggcagaa cgtgcacatc actacccttc
    20401 cgaactttct ggtggtcaac agcagcgtgt ggcgatcgca cgcgcgctgg cggtgaagcc
    20461 gaaaatgatg ctgtttgatg aaccgacttc cgctcttgac ccggaactgc gccatgaagt
    20521 gctgaaggtt atgcaggatc tggctgaaga agggatgacg atggtgatcg tgacacacga
    20581 aatcggtttt gccgagaaag tagcttcgcg cctgatcttt atcgacaaag gccggattgc
    20641 ggaagatggc gatccgcagg tgttgatcaa gaacccaccg agccagcgct tgcaggaatt
    20701 tttgcagcac gtctcttaac ttctcagcct ccctttcctc cgaaagggag gcacaccaga
    20761 ttcctctcat ttaaaatcgc ccctcctcca gcatctatac ttatcttttt gctctgtttt
    20821 ctcactggag gagtcatgcg gtggatcctg ttcatcctct tctgcctgct gggcgcacct
    20881 gcccacgcgg tatccatacc cggcgttaca accacaacga caacggactc aacgactgaa
    20941 ccggccccgg aaccggatat cgaacaaaaa aaagcggcct atggtgcact ggcggatgtg
    21001 ctggataatg acacctcacg taaagagttg atcgaccagt tgcgcaccgt cgccgcgacg
    21061 ccccctgcgg aacccgtacc gaagatcgtg ccgccgacgc tggttgaaga gcaaaccgtg
    21121 ctgcaaaaag tcaccgaagt cagccgccat tatggtgaag ccctttccgc ccgcttcgga
    21181 caactttatc gcaatatcac cggctccccg cataagccgt ttaatccaca aaccttcagc
    21241 aatgcgttga cccatttttc aatgttagcg gtattagtgt ttggttttta ctggctgatt
    21301 cgcctgtgcg cacttccgct gtatcgcaaa atgggccagt gggcgcggca aaaaaatcgt
    21361 gagcgcagta actggttgca gcttccggcg atgattatcg gggcgtttat tatcgacctg
    21421 ctgttactgg cactgacatt atttgtcggt caggttttaa gcgacaacct gaatgcgggc
    21481 agtcgcacca tcgctttcca acaaagtttg tttctcaacg cctttgccct gattgaattt
    21541 ttcaaagctg tactacgcct gattttttgc ccaaatgtgg cggagctgcg cccgttcacg
    21601 attcatgacg agaccgcccg ttactggagc cgtcgcctga gctggttaag cagtttgata
    21661 ggttatggcc tgattgtggc cgtgccgatt atctctaatc aggtgaatgt acaaattggc
    21721 gcgctggcga acgtcatcat tatgctgtgc atgaccatct gggcgttgta cctgatcttt
    21781 cgtaataaaa aagagattac ccaacatttg ctcaactttg cggagcattc gctggccttt
    21841 ttcagcttgt ttatccgcgc ctttgcgctg gtgtggcact ggctggcaag cgcctatttt
    21901 atcgtgctgt ttttcttttc gttgttcgat ccgggcaaca gcctgaaatt tatgatgggt
    21961 gcaacggtgc gcagcctggc gattattggt atcgcagcat ttgtttccgg tatgttttcc
    22021 cgctggctgg cgaaaaccat caccctctcg ccacatactc agcgaaatta tccggagctg
    22081 caaaaacggc tgaatggctg gctttcggcg gcgctgaaaa cggcgcgtat tctgaccgtc
    22141 tgcgtggcgg taatgctgct gttgagcgca tggggattat tcgatttctg gaactggctg
    22201 caaaacggtg cggggcagaa aaccgtagat atcctgatcc gtatcgcact cattcttttc
    22261 ttctcggcgg ttggctggac ggtgctcgcc agtttgatcg aaaaccggct ggcttcggat
    22321 attcatggcc gcccactacc cagcgcccgt acgcgtaccc tgctgacgct gttccgtaac
    22381 gcgctggcgg tgattatcag taccatcacc atcatgattg tgttgtcgga aatcggcgtt
    22441 aatatcgcgc cattgctggc aggtgccggg gcattaggtc tggctatctc ttttggttcg
    22501 caaacgctgg tgaaagatat tatcaccggg gtatttattc agtttgaaaa cggcatgaac
    22561 actggagatt tggtgactat cgggccgttg accggcactg tggaacggat gtcgattcgc
    22621 tccgtgggcg tgcgccagga taccggggcg tatcacatca ttccgtggtc ttcgataacc
    22681 acctttgcta acttcgtccg cggtattggt tcggtagtgg caaactacga tgttgatcgc
    22741 catgaagatg ctgataaagc caatcaggca ctgaaagatg cggtagcaga attgatggaa
    22801 aacgaagaaa ttcgcgggct ggttattggt gaaccgaact ttgccgggat tgtcggctta
    22861 agcaataccg cgtttacact gcgtgtttcg ttcaccacgc tgccactcaa acagtggacg
    22921 gtccgctttg ccctcgacag ccaggtgaaa aaacatttcg acctggcggg tgttcgcgcg
    22981 ccggtgcaga cttatcaggt gctgccagct ccgggcgcgg ccccggctga accgttgccg
    23041 ccgggggagc caacgcttta acgctggcga ttgacaaaac ggcggcgctg ctcgtcgttc
    23101 ataaaggtcc aggctataaa gcggctctgc ttttgcccct gggccatctc tttttttacc
    23161 accttcaccg cacctacgtc cgtcaaagca cgatacaacg gcggtaagtt ttcaccacga
    23221 gataccagcg aggtaaacca catcacctgc ttcgcgaagc ctttgctctc ttcaatcatc
    23281 tttttgataa aagcgacctc accgccttca caccacaact cctgttgctg accaccaaag
    23341 ttcagtgcat cgtctttgtt cagccccagg ttacggcgtt tacgttcact acctgcccgt
    23401 gccgcagcgg cggaatcgtg gaatggcggg ttacacaagg tcgcatcgta ttgctcgttt
    23461 ttatggatga tgccgttaaa aatcgcccca ctctcttttt gccgacgcag acgaatggcg
    23521 cggttaagcc ccggattagc actgatgatc gcctgcgcac tgcttaacgc ctggctgctg
    23581 gtttcactac cggtaaaacg ccagccatat tcatgtacgc caatcagcgg ataaatacag
    23641 ttcgcaccaa caccgatatc cagaatgctg gcattagctg gaattgttcc gcttgcttca
    23701 gccagtaaat cggcaaggtg atgaatataa tccgcccggc ccggtactgg cgggcagaga
    23761 aaaccgtccg ggatatccca gttcgctacg gcgtaaaaat gggccagcaa cgccttattg
    23821 agcgccttca ccgccagcgg attggcaaag tctacgcttt gctccccggc gggtgtaagc
    23881 gtgaggaatt gcctgagttc aggattgacc tgacaaagcg tggcgagatc gtagcggctg
    23941 tgatgacggt tgcgcggatg caaccccggt ttctgggcgg acatagcatt ctcctttaag
    24001 cagcggcgta agatacccgc ataacgcgac agggtaaata tctggctgcg tttatcattt
    24061 ctaaaaacct tcacatggct atggtttatg gtaattcacc ataaaaatca tcatcatgca
    24121 tgtcattgat ttatcttatt ttgtaaaaat atattgttca aaaaataagc gcatctctgt
    24181 caattttttg cgtcattttc atcgcgcagg cgacctacac ttatctggca ctacccgttg
    24241 tatttatgag gatgtcatta tgaaaaagtg cctcacacta ctgattgcca ccgtcctgag
    24301 cggaatctct ctcacggctt atgccgcgca accgatgagt aacctggaca gtggtcaact
    24361 acggcccgcc ggtaccgttt cggcaactgg cgcatcaaac ctaagcgatc tggaggataa
    24421 actggcagaa aaagcgcgcg aacaaggcgc gaaaggttat gtcattaatt ccgcaggcgg
    24481 aaatgaccag atgttcggta ctgcaatcat ctacaaataa ccccaccaac gtaactgcat
    24541 tcgcctctgg atgcagtttt cgtccaattt gtaagcaaaa aacatcaaat atgaacgcaa
    24601 tgtaaataaa tgtatttctt tttcgcgcaa tgggtgatag aaaatcgcgc caagtgataa
    24661 tgcttatcaa aattattatc acttgcacgg acgctatcac gggattaaca gtggcatcgc
    24721 atccgcagag atgctttctc gtgacagtga aaatttcacc atataagaaa aagtcacctg
    24781 caaaatggaa aacaatcgca atttccctgc cagacaattt cattcgctca cgttctttgc
    24841 cggtctttgt attggtatta cgcctgtggc gcaggcactc gctgccgaag ggcaagctaa
    24901 cgcggatgac acgctggttg tcgaagcatc aacgccttcg ctttatgcgc cacaacaatc
    24961 tgccgatccg aaattctcgc gtccggtagc ggatactacc cgcacgatga cggtgatttc
    25021 tgaacaagtg attaaagatc agggtgcaac caaccttacc gacgcgctca aaaacgtccc
    25081 cggcgtgggt gcgttttttg cgggtgagaa cggtaactcc accactggcg acgccattta
    25141 tatgcgcggt gccgatacct ctaacagtat ttatatcgat ggcattcgcg atatcggcag
    25201 cgtctcgcgc gacaccttca ataccgagca ggtcgaagta attaaagggc cgtccggcac
    25261 cgactacggg cgcagcgcgc cgacaggctc gatcaatatg atcagcaaac agccgcgcaa
    25321 tgattccggc attgacgcct ccgccagtat tggcagcgcc tggttccgcc gcggcacgct
    25381 ggacgtcaat caggtcattg gtgataccac tgcggtgcgc ctgaatgtaa tgggcgaaaa
    25441 aacgcacgat gccggacgcg acaaagtcaa aaatgagcgt tacggcgtcg ccccttctgt
    25501 cgcttttggc cttggtacag cgaatcgttt gtatcttaat tatctgcatg tcacccagca
    25561 caacacgcca gacggcggca ttccgactat cggtttaccg ggttattctg ccccatctgc
    25621 gggaacagcg gctctgaatc attccggaaa agttgatact cataactttt acggcacgga
    25681 ttccgattac gacgattcga ccaccgacac cgctaccatg cgttttgagc acgacatcaa
    25741 cgataacacc accattcgca atactacccg ttggtcgcgc gtgaagcagg attacctgat
    25801 gacggcgatt atgggcgggg cgtcgaatat tacccagccc accagcgatg tgaatagctg
    25861 gacctggtca cgcacggcga atactaaaga tgtgagtaat aaaattctca ccaaccagac
    25921 caacctgacc tcgacgttct ataccggcgc tatcggtcat gatgtcagta ccggcgtgga
    25981 atttacccgt gaaacgcaga ctaactacgg cgttaatccg gtgacgttac ctgcggtaaa
    26041 tatttatcat cctgacagca gcattcatcc cggcggcctg acgcgcaacg gcgcaaacgc
    26101 caatggtcag acggatacct tcgcaattta cgccttcgat acgctgcaaa tcacccgtga
    26161 ttttgagctg aacggcggga tccgtctgga taattatcat actgaatatg acagtgccac
    26221 cgcctgcggc ggcagcggac gcggtgccat cacctgccct gctggcgtgg caaaaggttc
    26281 tccggtcacc accgtcgata ccgccaagtc gggcaatctg gtgaactgga aagccggggc
    26341 gctgtatcac ctgacggaaa acggcaatgt ctatattaac tatgccgttt cccagcagcc
    26401 tccgggcggc aacaacttcg cccttgcgca gtctggcagc ggtaacagtg ccaaccgcac
    26461 cgattttaaa ccgcaaaaag ccaacaccag cgagattggc accaaatggc aggttctgga
    26521 taaacgcctg ttgctcaccg ccgcgctgtt ccgtactgat atcaaaaatg aagttgagca
    26581 aaatgatgac ggaacttact cgcaatacgg taagaaacgc gtcgaaggct atgagatatc
    26641 cgtggccggg aatatcactc ccgcgtggca ggtgattggc ggctataccc agcaaaaagc
    26701 aaccatcaaa aacggcaaag atgttgccca ggatggttcc tcatcgctgc cgtatacccc
    26761 ggagcacgcc tttaccttat ggagccagta tcaggcaacc gatgatattt ccgttggtgc
    26821 gggcgcacgc tatatcggca gtatgcataa aggttctgac ggcgcggtgg gaacgccagc
    26881 gtttaccgaa ggttattggg tcgccgacgc taaactgggg tatcgggtta atcgcaatct
    26941 cgacttccag ctaaacgtct acaacctgtt tgataccgat tacgtcgcct caatcaacaa
    27001 gagcggctac cgttatcacc cgggcgagcc aagaaccttc ttgctcacgg ccaatatgca
    27061 tttctgattg agatgtgggg cgcaggcccc actttttgga gaacttgtat gatgtaccac
    27121 attcccggcg tgctatcgcc acaggacgtc gcccgttttc gcgaacaact ggaacaagcc
    27181 gaatgggtgg atggacgcgt caccactggc gcacaaggtg cgcaagttaa gaacaatcaa
    27241 caggtagaca cccgcagcac gttatacgcc gccctgcaaa atgaggtgct gaacgcggtt
    27301 aaccaacatg ctttgttctt tgccgcggcc ttgccgcgta ccctttccac gccgctgttt
    27361 aatcgctatc agaacaatga aacctatggt tttcatgtgg atggcgcagt acgcagtcac
    27421 ccacaaaacg gctggatgcg cactgacctt tctgccacgc tgtttttaag cgatccacaa
    27481 agctacgatg gcggcgaact ggtcgttaat gacaccttcg ggcaacatcg ggtaaaactt
    27541 ccggcaggcg atctggtgtt gtatccctcc agcagcctgc attgcgtgac acccgtaacc
    27601 cgcggcgtac gagtggcatc atttatgtgg atccagtcga tgatccgcga tgataaaaag
    27661 cgcgccatgc tgtttgaact ggacaacaat attcagtcgc tgaaaagccg ctacggcgaa
    27721 agtgaagaga tcctgtcgct gctcaatctt tatcataatc tgctgcggga atggtcggag
    27781 atctgacgct tgaactgccc gagttcgtcc ctatattagc tacacttaac tgtacaagta
    27841 ttgatatggg gaggacgata tggcatccgg ttgggctaac gatgacgccg tcaacgaaca
    27901 gatcaacagt acaattgaag atgcgattgc ccgcgctcgg ggtgaaattc cgcgtggcga
    27961 aagcctgtat gaatgtgaag agtgcggagc ccccatcccg caagcccgtc gggaagccat
    28021 tcctggtgtg cgcttatgca ttcattgtca gcaggagaaa gatttacaaa aaccagctta
    28081 tacaggatat aatcgcagag gttcgaaaga cagccagtta cgttaactac aactggcggg
    28141 atgtatagcg agtataattt gtattttgcg tggcattccg tgcctttaac gctgtcgtga
    28201 ctgccacgct gcataaaaca agtccataag ttcaacaaat aaatggatta tcagtatgtt
    28261 agatatcaat caatttattt gaacaaggcg gtcaattctc ttcgatttta tctctcgaaa
    28321 aaaaacgtga tactcatcac atcgacgaaa caacgtcact tatacaaaaa tcacctgcga
    28381 gagattaatt atgaaaacta tcaatactgt tgttgctgct atggctcttt caactctgtc
    28441 atttggcgtg tttgccgcgg aaccggtaac ggcatcccag gcacagaaca tgaataaaat
    28501 cggcgtggtt tctgccgatg gcgcatccac cctcgatgcc ctggaagcga aactggctga
    28561 gaaagctgcg gcagctgggg cgagtggata cagcatcact tctgccacca acaacaataa
    28621 attaagcggt actgcggtaa tttataagta attcgtagaa cggataaggc gtttacgcta
    28681 cgtccagcac aagtatttcc ctgtctgttt gccgacagac gcatatgctc taaccctcat
    28741 tgatcctatg ttacccttgt ttgcccgtcc gccactggac gggctttttt ttagctggct
    28801 aactgctgac agaaagcctg caacgtctct tccggcatac caacctttcg cgccgcatca
    28861 caaatggcct gccagcttcc cgcatccaat ggaatcccct gctcctgccg ttcgcgacgc
    28921 gtgttaactt cccactcgcc cggtagcaaa atcggcttat cgtcatcatg cggcgaggct
    28981 ttcacccact cggcaaaggc ttcggtctgc gcgctacaat ccggcgcgcc gaacagttcc
    29041 gggttgatga tgatagtggt catgcagtta agaatggcat cgggactggt ttgtaacgtt
    29101 ttctgatgcg tcgttttacc gccggaaagc gccccaccaa gaatttcaca catcgctgca
    29161 agggcgtagc ctttatgttc ggcaaaggtc aacagcgaac ccaacggcga ctcctgcatt
    29221 accgccggat tggtcgtcgg catgccgtta acgtcaatca ggcaacctgg cggcacgggg
    29281 acgcctttat gccaggcgac gcgggttttg ccaaatgcga tggcgctggt ggcgtaatca
    29341 agcaacagcg ggaaattatc tttacgaggg aaaaccacac agaacggatt ggtgccaaag
    29401 cggctgtcgc gaccgtggaa cggcgcgacc attggaatac cgaccacgct aacaaagtgg
    29461 atagagacaa accccgccgc tgcacactgc tccgcccagt aaccgatacg gccgatatga
    29521 tgcgagttat gtagcgccac ggcagcaata ccgtgctgat gcgctttctc aatccccagc
    29581 gccatcgctt cgtgtgccgc gacctgacca aatgcgcgat cgccatcaag cgtaaccacc
    29641 gcgcccgctt ctttaacgat tttggcatgg tggttaattt gcagatgccc ctgactccag
    29701 gaacgcacat agcttgggat catgccaata ccatgtgaat catgccctgc caggtttgcc
    29761 gcgattaaat gatcggcaac taatttcgct tcttgttcct cgctacccat ctgacgaaat
    29821 acagcctgaa taaaactgtg cagcgtctga gcatcaaagc gatgaccact ttccatggtt
    29881 tactcctgtt tatgttatgt gtttgttgtt tttttatgct gcacgccggg cgcgggaagg
    29941 tcaataaaga tgcaggaatt tcatattgcg gtggggtaaa agccggatga catggctcat
    30001 ccggctgaga taaaaattaa aatgcctgat taatgcgcgc caggccgtcg ttgatagttg
    30061 ccgcttcacc cgtagccatc aggcagcaag ccatctggat tttcagcgat tcaggaatcg
    30121 gttcgctgcc agcaaggcaa cgcttaatcc actgcgccgt ggtttccgga tcttttgctt
    30181 gtggcagtaa ctcgctacca gcagtgtcct gtttttcata cagtacccgc atcccttcac
    30241 ggtcaatgag attgatctgc gggcagcgct gcggattagc atacacttca ccttcagtac
    30301 catgcattag tagcgcccgc ccgccgatat cactaaagaa cttcgcgacg cgtccaatgt
    30361 attccggatg cgaaacgctg gaaagacgca gtgcctcacc ttcggcaaat ggcgtcgcca
    30421 gtttcgccag ggtatgtgca ctgttacgca cgcccatccg ccagcgcatc gccagttgtt
    30481 tttccagcgg cgggcaaaac gcgccgacag gcataaacac cggttgatgt tcgtcgagct
    30541 tcgcctgcgc ctgcccgccg tgcaacgttg gcgtaatacc catcagttcg aaaatggttt
    30601 ctgtcagcac gcgggttgga tcttcgctaa ccccgtgaac caccacagga aaaccgagtt
    30661 tgtgcaggag aatcgccaac aacggcgtca ggttggcctg tttacgcgcg ccgttgtaac
    30721 tggggatgac aatcggcatc ggcttgtctg caggcggcgt cagcttgatg gtgtgatttt
    30781 gcatggcttc gtaaaagccg agcatctctg cttccccttc ccctttgata cgcagcgcaa
    30841 tcaatacgcc gcccaactca aggtcaggga cttcaccatt gagcatatgg gcatacagac
    30901 cgcgcgcagt atcccggtct aaatcgcgcg cgtggttttt cccgcgcccg atctctttaa
    30961 tgattttgcg atagtccatt tacgactcct tacctgactc acatcattaa cgccgccgac
    31021 ggcgtgggga tttcgttttt tctttctttt taactatacc ttcagggact tccggttgct
    31081 ctatcggaaa taccggtaat gcatccagta gtcgcttgcc ataatttttg gtcagcaagc
    31141 gtttgtcgta gataaccact tccccccagc aaccgtggct tcgaatcagt cgcccaacct
    31201 gctgaatcag gttaaacgaa gcgctcggca ggctttgcac ctcaaacgga tagcggttga
    31261 ggcttttcag ccattcacct tcggtgatca ccaccggact gtcgatgggc ggaaaagcga
    31321 tcttgtggat atgcacctga ctgagcagat cgcctttcag atcaagacct tcggcaaagg
    31381 actgtaagcc caccagcacg ctacgctcac cgttggcaac gcgtttgcgg tgcagttcaa
    31441 ctaagcgata acgtggctga tcgccctgca ccagcaacat caggcgtaaa tccgtcacat
    31501 agtcgagaaa gcgctgcatc gcacgcccgc tggcaaacag taccaacata ccgagatgtt
    31561 ttttgctctc cacctgctca cggaaaaagg ctgccatttc agcaatgtgc tgctcttcgt
    31621 tgtcgatgga aggctcaaag cgcatccggg gaataacaat tttgccctgt tcgcagtggt
    31681 taaagggcga atccagcgcc acaaagcggt cgcccgcttt ctctttcaga ccactcatct
    31741 cctgtaaacg cgaaaaactg ttcagcgaac gtaaggtcgc ggaagtgaca ataatgtgcg
    31801 gaatactgcg ccacagcagc ctttccagct gatcgctgac acgaataccc acacagtgaa
    31861 accagagatg cagctgccct tcgcgctctt cccgcgtcgc ccatttagtc accggcgcac
    31921 cggaagattg cgccagagac gccagccgcc agagtttgct ttgcgcctcg aacatcccca
    31981 acgcgcgatt catctgcaaa atcaaccgat gcagacgtac aatgtcatgg ctaccggttt
    32041 tctcacttaa atcgttaaga aataactccg ccaggccacg cagcatctcg gtgagttttg
    32101 ccagccgctg gcagatctcc agcacttcat caggcagttc gcccatcgca aaacggtgct
    32161 ctgcctcctg cccggcaggc atgtagagat tgagaatgtt gtttaacgag gcgataagct
    32221 catacagctc ttcacaatgc gcattcaaac gttcagggat cgccagcggt gggatggtct
    32281 tcgggcgaaa ctgctccatg caggtagcga ccagtttcgt gaacaagtcc agctgtagcc
    32341 gataccaggg cgcggtgatt tcggcgctca tctccagcgc atcccgcgcc acatctggca
    32401 ggtgatgacc ttcgtccagc accagcagca gatttttcgg gtcaggtagt acggcttcgc
    32461 tttccatcgc cgccatcacc agcgcatggt ttgccaccac cacttccgct tcctgaatct
    32521 cccgacgagc aacaaaaaac gggcattcac gatagtagta acagttgcgg ttgaggcagc
    32581 tggctttgtc ggtacttaaa cgacgccaga gatcgtcatc aatggcgata tccgtatgat
    32641 cgcgcagacc atcccattta taagtgtcga gatcgccctt cagcttcgca caacgttttt
    32701 gctcttcctg attgttcggc gtcagctcgt cgtcaagaaa cgccagcaga tcctgttgcg
    32761 tgggttcagt actggcaagc gctgtcagat tacgcggaca aacgtagcgc ccacgcccaa
    32821 aagcagcagt gaatttaaga tcgggaatga tctttttcag cagaggcaaa tctttactgt
    32881 agatctgatc ctgcaatgcc acgttggcgg tactcaccac cagcgttttt tgctcttcgc
    32941 gggcaatggc gatgccggga atcaaatagg agagcgtttt cccaacgccg gtgggtgctt
    33001 caatcgccag atgccgccct tcttctccgg ccagcgtttt ggcgacgtcc gcaatcatct
    33061 gccgctgcgg cgcacgggga ataaagtcgg ggatctgttc ctgaagcgcc ttataccagg
    33121 cggcaatttg cgctttaagc gcggcggtta atgccatgag aaaacctgaa atactgtata
    33181 aacagccaat attgtggcat ttttcggttt tcaggggtaa tggtttttct gtttctggaa
    33241 agcggctcgc aataaccagc aggatgatgc gtaaagcgct tcgcgctaaa ttcgaacaac
    33301 atgcagaact gcgtacactc ttgctggcaa ccgcgtccgc aaaactggtt gagcatacgc
    33361 aaaatgatgc ttactgggga gatagcggta atggtcaggg caagaatcgc ctggggtacc
    33421 ttttaatggc gttgcgcgga caattggctg cggagaagta acgcatcggg tggcttgtgg
    33481 gcgttaaaac agagaaatct ttttgccgcg ctcgcaaatg gctgacaagt gcttgttgtg
    33541 aaatgccgga tgcggcgtaa acgccttatc cggcctacaa aagcatgcaa attcaatata
    33601 ttacagagtt cacgtaggcc tgataagcgc agcgcatcag gcagttttgc gtttgttcat
    33661 cagcctgatg ccgggcatcg cccggcatca aaagattact gcgcagcggc aggtttacgt
    33721 ggacggcggc gacgttgttg ttcgcctgcc ggtttggcat cgccgaggcg gcgagacggt
    33781 ttttctgcag gtttcgcact tgcagatttt gcgccacctt ctccacggcg tggttgctgt
    33841 tgaccgcgac caccaccttg cccacgaccg ccgccacgtt gctggcgacc gttctggatc
    33901 ggttcggctt tgattgacgg gtccggctca taacccggaa tcgcaatgcg cgggatctct
    33961 tttttcagca gtttttcgat atcacgcagc agtttgtgtt catcaacaca caccagcgac
    34021 aacgcttcac cggtagcagc cgcacgaccg gtacgcccga tacggtggac ataatcttcc
    34081 ggtacgtttg gcagttcata gttgaccacg tgcggcagct cttcaatatc caggccgcgc
    34141 gcagcgatgt cagttgccac cagtacacga atatcgcccg atttaaaatc agccagcgca
    34201 cgagtacgcg caccttgcga tttattaccg tggatcgccg cactacggat gccatcttta
    34261 ttaagctgtt cagccagatg gttagcgcca tgtttggtac gggtaaacac cagcacctgc
    34321 tgccagttcc ctttcccaat catatgcgac agcaattcgc gtttgcgttt cttatcgaca
    34381 aagtgaacgt gctgggtcac ctgatcagac gcggtattgc ggcgtgccac ttcgatttcc
    34441 agcgggttgt gcaacagttt ttccgccagg gctttaatat cgtcagagaa ggtcgcggag
    34501 aacaacaggt tctggcgctt cgctggcagt tttgttaaca cgcgacggat atcgtggata
    34561 aagcccatgt cgagcatgcg gtcagcttca tcaaggacga ggatttcaac ctgatccagc
    34621 ttcactgcat tttgatgttc caggtccagc agacgtcccg gcgttgccac cagcacatca
    34681 acgccgccgc gcaacttcat catttgtggg ttaatgctga caccgccaaa caccaccagc
    34741 gaacgaatgt tcagatattt gctgtaatca cggacgtttt cgccaatctg cgccgccagt
    34801 tcacgggtcg gggtaagaat gagcgcgcgt accggacgac gccctttggc gtgcggctgg
    34861 cgagtgatca ggtgttgcaa cagcggcagc gtaaagcccg ctgttttgcc ggtaccggtc
    34921 tgggcgctgg ccatcaggtc gcggccttcc agcaccgcag ggatcgcctg ctgctgaata
    34981 ggggtgggtt cacggtaacc ctgctcggca acggcgcgca ggatatcagg gcttaaaccc
    35041 aaagaatcga aagacataac tactccgaac cgccccgacc gttaccggtg tagttttcag
    35101 ggagatactg aaaaggaaaa tgacaaaaac cacaatgtca tgagggagcg gagtgtagca
    35161 gtttttgtga cgcagcgcat aaattatccc tgtgactggc gttgctaaaa ttcttagcca
    35221 tactggacaa ctctcatatg gagtcataat cttaatcaat catttgatta agaatggact
    35281 gtgcgatgaa taatcctgcc atgacaatca agggtgaaca ggcgaagaaa cagctgattg
    35341 ctgccgcact ggcgcagttt ggtgaatatg gaatgaacgc caccacccgc gagatagccg
    35401 cccaggccgg gcagaatatc gctgccatca cctattactt cggttcgaaa gaggatttgt
    35461 acctcgcctg cgcccagtgg attgccgatt ttattggcga gcagttccgt ccacacgccg
    35521 aagaagccga acgtttgttc gcgcaaccac agcctgatcg cgcagctatc cgtgaactga
    35581 tccttcgcgc ctgcaggaac atgattaagc tgctcaccca ggatgacacc gtcaacctca
    35641 gcaaatttat ctcccgtgag cagctctctc ccacggcagc ctatcacctg gtgcacgaac
    35701 aggtgattag tccgctacac agccacctta cacgcctgat tgccgcctgg accggctgcg
    35761 acgctagcga tacccgcatg atccttcata cccatgcgtt gattggtgag attctggcgt
    35821 ttcgtcttgg caaagaaact attctgttac gtaccggctg gaccgcgttc gatgaagaaa
    35881 agaccgaact gatcaaccag acggtgacct gtcatattga cctgattttg caaggattat
    35941 cgcaaaggag tttgtagtga tgaaaaaacc tgtcgtgatc ggactggcgg tagtggtact
    36001 tgccgccgtg gttgccggag gctactggtg gtatcaaagc cgccaggata acggcctgac
    36061 gctgtatggc aacgtggata ttcgtacggt gaatcttagt ttccgtgttg gggggcgcgt
    36121 tgagtcgctg gcggtggacg aaggtgatgc gatcaaagcg ggccaggtac tgggcgaact
    36181 ggatcacaag ccgtatgaga ttgccctgat gcaggcgaaa gcgggcgttt cggtggctca
    36241 ggcgcagtat gacctgatgc ttgccgggta tcgagatgaa gaaatcgctc aggccgccgc
    36301 agcggtgaaa caggcgcaag ctgcctatga ctatgcgcag aacttctata accgccagca
    36361 aggactgtgg aaaagccgca ccatttcggc aaatgacctg gaaaatgccc gctcctcgcg
    36421 cgaccaggcg caggccacgc tgaaatcagc gcaggataaa ttgcgtcagt accgttccgg
    36481 taaccgcgaa caggacatcg cccaggcgaa agccagcctc gaacaagcgc aggcgcaact
    36541 ggcgcaggcg gagctgaatt tacaggactc aacgttgata gccccgtctg atggcacgct
    36601 gttaacgcgc gcggtggagc ctggcacggt gctcaatgaa ggtggcacag tctttaccgt
    36661 ctcactgaca cgtccggtgt gggtacgtgc ttatgttgat gaacgtaatc tcgaccaggc
    36721 ccagccgggg cgcaaagtgc tgctttatac cgacggtcgc ccggacaaac cgtatcacgg
    36781 gcagattggt ttcgtttcgc ctaccgctga atttaccccg aaaactgtcg aaacgccgga
    36841 tctgcgtacc gacctcgtct atcgcctgcg tattgtggtg accgacgccg atgatgcctt
    36901 acgccaggga atgccggtga cggtgcaatt cggtaacgag gcaggacatg aatgatgccg
    36961 ttattacgct gaacggtctg gaaaaacgct ttccgggcat ggacaagccc gccgtcgcgc
    37021 cgctcgactg taccattcat gctggttatg taacggggct ggtggggccg gatggcgcag
    37081 gtaaaaccac actgatgcgc atgctggcgg gattactgaa acccgatagc ggcagcgcca
    37141 cggtgattgg ctttgatccg atcaaaaacg acggcgcgct gcacgccgtg ctcggctata
    37201 tgccgcagaa attcggcctg tatgaagatc tgacggtgat ggagaacctc aatctgtacg
    37261 cggatttgcg cagcgtcacc ggtgaagcgc ggaagcagac ttttgctcgc ctgctggaat
    37321 ttacatctct tggcccgttt accggacgcc tggcgggcaa gctctccggc ggaatgaagc
    37381 aaaaacttgg cctggcctgt accctggtgg gcgaaccgaa agtgttgctg ctcgatgaac
    37441 ccggcgtcgg cgttgaccct atctcacgcc gcgaactgtg gcagatggtg catgagctgg
    37501 caggcgaagg aatgttaatt ctctggagca cctcgtatct cgacgaagcc gaacagtgcc
    37561 gtgacgtgtt actgatgaac gaaggcgagc tgctgtatca gggagaaccg acggccctga
    37621 ctcaaactat ggccggacgc agctttctga tgaccagccc gcacgagggc aaccgcaaac
    37681 tgttgcaacg ggcattgaaa ctgccgcagg tcagcgacgg catgattcag gggaaatcgg
    37741 tacgtctgat cctcaaaaaa gagaccacgc cagacgatat tcgccatgcc gacgggatgc
    37801 cggaaatcaa catcaacgaa actacgccgc gttttgaaga tgcgtttatt gatttgctgg
    37861 gcggtgccgg aacctcggaa tcgccgctgg gcgcaatatt acatacggta gaaggcacac
    37921 ccggcgagac ggtgatcgaa gcgaaagagc tgaccaaaaa gtttggcgat tttgccgcca
    37981 ccgatcacgt caactttgcc gtcaaacgcg gggagatttt tggtttgctg gggccaaacg
    38041 gcgcgggtaa atcgaccacc tttaagatga tgtgcggttt gctggtgccg acctctggtc
    38101 aggcgctggt gctgggcatg gatctgaaag agagttccgg taaagcgcgc caacatctcg
    38161 gctatatggc gcaaaaattt tcgctctatg gcaacctgac ggtcgaacag aatttacgct
    38221 ttttctctgg tgtgtatggc ttacgcggtc gggcgcagaa cgaaaaaatc tcccgtatga
    38281 gcgaggcgtt cggcctgaaa agtatcgcct cccacgcgac cgatgaactg ccattaggtt
    38341 ttaaacagcg gctggcgctg gcctgttcac tgatgcatga accggacatt ctgtttctcg
    38401 acgaaccgac gtccggcgtt gatcccctca cccgccgtga attttggcta catatcaaca
    38461 gtatggtaga gaaaggcgtc acggtgatgg tcaccaccca ctttatggat gaagcggaat
    38521 attgcgaccg catcggcctg gtgtaccgcg ggaaattaat cgccagcggc acaccggacg
    38581 atttgaaagc gcagtcggcc aacgatgagc aacccgatcc cacgatggag caagccttta
    38641 ttcagttgat ccacgactgg gataaggagc atagcaatga gtaatccgat cctgtcctgg
    38701 cgtcgcgtgc gggcgctgtg cgtcaaagag acgcggcaga tcgttcgcga tccgagtagc
    38761 tggctgattg cggtagtgat cccactgcta ctgctgttta tttttggata cggcattaac
    38821 ctcgactcca gcaagctgcg ggtcgggatt ttactggaac agcgtagcga agcggcgctg
    38881 gatttcaccc acaccatgac cggttcgccc tacatcgacg ccaccatcag cgataaccgt
    38941 caggaactga tcgccaaaat gcaggcgggg aaaattcgcg gtctggtggt tattccggtg
    39001 gatttcgccg aacagatgga gcgcgccaac gccaccgcac cgattcaggt gatcaccgac
    39061 ggcagtgagc cgaataccgc taactttgtg caggggtatg tcgaagggat ctggcagatc
    39121 tggcaaatgc agcgagcgga ggataacggg cagacttttg aaccgcttat tgatgtacaa
    39181 acccgctact ggtttaaccc ggcggcaata agccagcact ttattatccc cggtgcggtg
    39241 accattatca tgacggtcat cggcgcgatt ctcacctcgc tggtggtggc gcgagaatgg
    39301 gaacgcggca ccatggaggc gttactctct acagagatta cccgcacgga actgctgctg
    39361 tgtaaactga tcccttatta ctttctcggg atgctggcga tgttgctgtg tatgctggtg
    39421 tcagtgttta ttctcggcgt gccgtatcgc ggttcgctgc tgattctgtt ttttatctcc
    39481 agcctgtttt tactcagtac cctggggatg gggctgctga tttccacgat aacccgcaac
    39541 cagttcaatg ccgctcaggt cgctctgaac gcggcttttc tgccgtcgat tatgctttcc
    39601 ggctttattt ttcaaatcga cagtatgccc gcggtgatcc gcgcggtgac gtacattatt
    39661 cccgctcgtt atttcgtcag caccctgcaa agcctgttcc tcgccgggaa tattccagtg
    39721 gtgctggtgg taaacgtgct gtttttgatc gcttcggcgg tgatgtttat cggcctgacg
    39781 tggctgaaaa ccaaacgtcg gctggattag ggagaagagc atgtttcatc gcttatggac
    39841 gttaatccgc aaagagttgc agtcgctgct gcgcgaaccg caaacccgtg cgattcttat
    39901 tttacccgtg ctgattcagg tgatcctgtt cccgttcgcc gccacgctgg aagtgaccaa
    39961 cgccaccatc gccatctacg atgaagataa cggcgaacac tcggtagaac ttactcaacg
    40021 ttttgcccgc gccagcgcct ttacccatgt gctgctgttg aaaagcccgc aggagatccg
    40081 cccaaccatc gacacacaaa aggcattact cctggtgcgt ttcccggctg acttctcgcg
    40141 caaactggat accttccaga ccgcgccgtt gcagttgatc ctcgacgggc gtaactccaa
    40201 cagtgcgcaa attgccgcca actacctgca acagatcgtc aaaaattatc agcaggagct
    40261 gctggaagga aaaccgaaac ctaacaacag cgagctggtg gtacgcaact ggtataaccc
    40321 gaatctcgac tacaaatggt ttgtggtgcc gtcgctgatc gccatgatca ccactatcgg
    40381 cgtaatgatc gtcacttcac tttctgtcgc ccgcgaacgt gaacaaggta cgctcgatca
    40441 gttactggtc tcaccgctca ccacctggca gatcttcatc ggcaaggccg taccggcatt
    40501 aattgtcgcc accttccagg ccaccattgt gctggcgatt ggtatctggg cgtatcaaat
    40561 ccccttcgcc ggatcgctgg cgctgttcta ctttacgatg gtgatttacg gtttatcgct
    40621 ggtgggattc ggtctgttga tttcatcact ctgttcaaca caacagcagg cgtttatcgg
    40681 cgtgtttgtc tttatgatgc ccgccattct cctttccggt tacgtttcgc cggtggaaaa
    40741 catgccagta tggctgcaaa acctgacgtg gattaaccct attcgccact ttacggacat
    40801 taccaagcag atttatttga aagatgcgag tctggatatt gtgtggaata gtttgtggcc
    40861 gctactggtg ataacggcca cgacagggtc agcggcgtac gcgatgttta gacgtaaggt
    40921 gatgtaactt cttatctttc gccagcaaag acactaccgc cgggccagca aggattgcca
    40981 gccctgcaat cgccagcaaa aagttgtttt ccagtacgcg ggacaacagc cacagcacaa
    41041 tcagcgctgc gccaaaatac caggtcgtgg tcagctcttc cagcacgtct gccacctcac
    41101 gccagcgttg ctcggggtgg cgctgaaaca ccagcatcat cactaccgtg acacaataca
    41161 gtgcgcataa tcccacgccg acgtgaacca gagtcttact catccagccc accaccagca
    41221 gcgccactac cagtaaatgc aacataatct gccagcaact tagacccgtt gcgacacgaa
    41281 cacgttgttg ccacttcatg gcttctctcc tgaagaattt atctctactt actcagagta
    41341 ccgaacttta cggaaaattc cgtaagaccg cacctttttg tgacgccgac tacactattt
    41401 caatgagata agaggcaaag acaggaaaat tatgcccgat caaacacaac aattttcgtt
    41461 caaggtgctc accatcaata ttcacaaagg ctttaccgcg tttaaccgac gcttcatttt
    41521 gccggaactt cgcgacgccg tgcgtaccgt cagcgccgat attgtttgcc tgcaggaagt
    41581 gatgggcgcg cacgaagttc atccgctgca tgtggaaaac tggcccgata cctcgcacta
    41641 cgagtttctc gccgacacca tgtggagcga tttcgcctac ggtcgcaatg ccgtataccc
    41701 ggaagggcat cacggcaacg cggttctttc acgttatccc attgaacatt atgagaatcg
    41761 cgatgtttcg gtcgatggtg cggaaaagcg cggcgtgctc tactgccgca ttgtgccgcc
    41821 gatgaccggg aaagcgattc atgtgatgtg cgtacatctg ggcctgcgtg aggcgcaccg
    41881 tcaggcgcag cttgcgatgc tcgccgaatg ggtgaatgag ctgccggacg gcgaaccagt
    41941 attggtggcg ggtgatttca acgactggcg gcaaaaagct aatcatccgt taaaaatgca
    42001 ggccgggctg gatgagatat ttacccgcgc ccacggacgc ccggcgcgca cgtttccggt
    42061 gcaatttcct ctactacgtc tggacaggat ctatgtcaaa aatgccagcg ccagcgcgcc
    42121 aaccgcgttg ccgctgcgga catggcgaca cctttctgat catgcccctt taagtgcgga
    42181 gattcattta tgaaatgtag ctggcgcgaa ggcaataaga tccagttgct ggaaaacggc
    42241 gagcaatatt atcccgcgat gtttaaggcg attggcgagg cgcaagaacg catcattctt
    42301 gaaacgttta tctggtttga ggatgacgtc ggcaaacagc tgcatgcggc gctactggca
    42361 gcagcgcaac gcggggtgaa agcggaagtc ttgctggatg gctacggttc gccggatctc
    42421 agcgatgagt ttgtcaatga actgacggca gctggcgtgg tatttcgcta ctacgatccc
    42481 cgtcctcgcc tttttggtat gcgcaccaat gtgtttcgcc ggatgcatcg caaaattgtg
    42541 gtgatcgacg cgcgtatcgc ctttattggc gggctgaatt actccgccga gcatatgtcc
    42601 agctacggtc cagaggctaa acaggattac gcggtacgcc ttgaagggcc gattgtcgaa
    42661 gatattctcc agtttgagct ggaaaacctg cctggacaga gcgcggcacg acgctggtgg
    42721 cgacgtcatc acaaagcgga agagaaccgc cagccgggag aagcgcaggt attgctggtc
    42781 tggcgcgata acgaagaaca tcgcgatgat attgaacgtc actatctgaa aatgctcact
    42841 caggcgcggc gagaagtcat tatcgccaac gcctacttct tccccggcta tcgattttta
    42901 cacgccttgc gtaaagcggc acggcgcggg gtgcggatca aactgatcat tcagggcgaa
    42961 ccggatatgc cgattgtcag agtcggtgcg cgcttgctgt ataactatct ggttaaaggc
    43021 ggcgttcagg tatttgagta ccgccgccgc ccgctccacg gcaaagtggc attgatggac
    43081 gatcactggg cgacagtagg gtccagtaat ctcgatccgc tcagtttgtc actgaatctc
    43141 gaagcaaatg tcatcatcca cgatcgtcat tttaaccaga cgctgcgcga taatctgaac
    43201 ggcattattg ccgcagattg tcagcaggtc gatgagacca tgctgccgaa acgcacctgg
    43261 tggaacctga ccaaaagcgt gctggcgttc cactttttac gccacttccc ggcgctggtt
    43321 ggctggcttc cggcacatac accacgtctg gcgcaggttg atccccccgc acaaccgaca
    43381 atggaaacgc aggatcgggt agaaactgaa aacacggggg taaaaccctg atgagtaaat
    43441 cacacccgcg ctggcgctta gcaaagaaga tcctcacctg gctgtttttt atcgcggtga
    43501 ttgtgttgct ggtggtctac gccaaaaaag tggactggga agaggtctgg aaggtcatcc
    43561 gcgactacaa tcgcgttgcg ctgctcagtg cggtcgggct ggtggtcgtc agctatctga
    43621 tttacggctg ctatgacctg ctcgcccgtt tctactgcgg tcacaaactg gcgaagcgcc
    43681 aggtgatgct ggtgtcgttt atctgctacg ccttcaacct gacgctcagt acctgggtcg
    43741 gcggcatagg tatgcgctat cgtttgtact cgcggctagg gttaccgggc agcactatta
    43801 cgcggatttt ctcgctcagt attaccacca actggctggg ctatattttg ctggccggga
    43861 ttatctttac cgcaggcgtg gtggagctgc cggatcactg gtatgtcgat caaaccacgc
    43921 tgcgtattct cggcattggc ttattgatga ttatcgcggt ttatttgtgg ttttgcgctt
    43981 tcgcgaagca ccgccatatg accatcaaag ggcaaaagct ggtgctgcct tcatggaaat
    44041 tcgccctcgc ccagatgctg atttccagcg ttaactggat ggtcatggga gcgataatct
    44101 ggctgttact tggtcaaagc gtgaactatt tctttgtact gggcgtgttg ctggttagta
    44161 gtattgccgg ggtgattgtg catattccgg cggggattgg tgtgctggaa gcggtgttta
    44221 tcgcactact ggctggggag catacctcca aaggtagcat tatcgccgcc ctactcgctt
    44281 accgtgtgct gtattacttt atcccactgc tgctggcgct gatctgttac ctgctactgg
    44341 aaagccaggc gaagaagctg cgggcgaaaa atgaagcggc gatgtgaagt tcggttaagt
    44401 tctttagtat gtgcatttac gactaatgaa aaaaccgcgt atgcctttgc cagacaagcg
    44461 ttatagctat cgcggcaagt atcccaaaga agccgataaa atagtaatag agtgttaatg
    44521 cacctgtaaa agcgcaacta accggactcc acaactcttc gctatggcat tttcctgcca
    44581 gttcaaggag cttatttcgt ccatgcgaga taatgcctga ccataaaaca atcattaagc
    44641 aacttattac ccaaatgggc cgttcactca ttaatgcggc attcctgatg atcaccaggg
    44701 ccagtcctgc ggttgtcacc aaacccaaag cggtaatgag ccatatgccg tttaatatcc
    44761 tggcttcgtg ctgaaaatct aatttattgc tgctttgcga gtatgattcc attcttttag
    44821 ttatccttag aaaaaatatt aatattcacc atcaataaat ttaaagccac gcatacaaaa
    44881 tacttttgta tttatggata cggaaaaca
//
LOCUS       DAZGHF010000022        43709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGHF010000022 DAZGHF010000000
VERSION     DAZGHF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.022"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(195..788)
                     /gene="tehB"
                     /locus_tag="ACHK3A_001363"
     CDS             complement(195..788)
                     /gene="tehB"
                     /locus_tag="ACHK3A_001363"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO8739475.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLENLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(785..1777)
                     /gene="tehA"
                     /locus_tag="ACHK3A_001364"
     CDS             complement(785..1777)
                     /gene="tehA"
                     /locus_tag="ACHK3A_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO8739476.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLIRTERAVLMKAEDKE"
     gene            1901..2881
                     /gene="ydcK"
                     /locus_tag="ACHK3A_001365"
     CDS             1901..2881
                     /gene="ydcK"
                     /locus_tag="ACHK3A_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO8739477.1"
                     /translation="MRKYRLSEEQRAFSYQDDGTKKSVLLRQIIAMSDFNDVIAGTAG
                     GWIDCETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIEKHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2873..3412)
                     /gene="rimL"
                     /locus_tag="ACHK3A_001366"
     CDS             complement(2873..3412)
                     /gene="rimL"
                     /locus_tag="ACHK3A_001366"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO8739478.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNRTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(3475..3699)
                     /gene="ydcH"
                     /locus_tag="ACHK3A_001367"
     CDS             complement(3475..3699)
                     /gene="ydcH"
                     /locus_tag="ACHK3A_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO8739479.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            complement(3839..5494)
                     /gene="opgD"
                     /locus_tag="ACHK3A_001368"
                     /gene_synonym="mdoD"
     CDS             complement(3839..5494)
                     /gene="opgD"
                     /locus_tag="ACHK3A_001368"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO8739480.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTDFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5719..7062)
                     /gene="ydcJ"
                     /locus_tag="ACHK3A_001369"
     CDS             complement(5719..7062)
                     /gene="ydcJ"
                     /locus_tag="ACHK3A_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO8739481.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGETHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7279..8202
                     /gene="ydcI"
                     /locus_tag="ACHK3A_001370"
     CDS             7279..8202
                     /gene="ydcI"
                     /locus_tag="ACHK3A_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8739482.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(8240..9880)
                     /gene="trg"
                     /locus_tag="ACHK3A_001371"
     CDS             complement(8240..9880)
                     /gene="trg"
                     /locus_tag="ACHK3A_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO8739483.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            10279..10428
                     /gene="hokB"
                     /locus_tag="ACHK3A_001372"
     CDS             10279..10428
                     /gene="hokB"
                     /locus_tag="ACHK3A_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO8739484.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(10406..10474)
                     /gene="yncP"
                     /locus_tag="ACHK3A_001373"
     CDS             complement(10406..10474)
                     /gene="yncP"
                     /locus_tag="ACHK3A_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO8739485.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(10500..10673)
                     /locus_tag="ACHK3A_001374"
     CDS             complement(10500..10673)
                     /locus_tag="ACHK3A_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739486.1"
                     /translation="MKKLALILVIGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCIN"
     gene            complement(10918..11448)
                     /gene="cybB"
                     /locus_tag="ACHK3A_001375"
     CDS             complement(10918..11448)
                     /gene="cybB"
                     /locus_tag="ACHK3A_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO8739487.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            11637..12638
                     /gene="gap"
                     /locus_tag="ACHK3A_001376"
     CDS             11637..12638
                     /gene="gap"
                     /locus_tag="ACHK3A_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO8739488.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILAKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(12679..14118)
                     /gene="aldA"
                     /locus_tag="ACHK3A_001377"
     CDS             complement(12679..14118)
                     /gene="aldA"
                     /locus_tag="ACHK3A_001377"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO8739489.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISTLIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(14315..15115)
                     /gene="ydcF"
                     /locus_tag="ACHK3A_001378"
     CDS             complement(14315..15115)
                     /gene="ydcF"
                     /locus_tag="ACHK3A_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO8739490.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEMPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(15387..19289)
                     /gene="hrpA"
                     /locus_tag="ACHK3A_001379"
     CDS             complement(15387..19289)
                     /gene="hrpA"
                     /locus_tag="ACHK3A_001379"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO8739491.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELSLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            19490..20095
                     /gene="azoR"
                     /locus_tag="ACHK3A_001380"
     CDS             19490..20095
                     /gene="azoR"
                     /locus_tag="ACHK3A_001380"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO8739492.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            complement(20149..21441)
                     /gene="ynbD"
                     /locus_tag="ACHK3A_001381"
                     /pseudo
     CDS             complement(20149..21441)
                     /gene="ynbD"
                     /locus_tag="ACHK3A_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
     gene            complement(21455..23212)
                     /gene="ynbC"
                     /locus_tag="ACHK3A_001382"
     CDS             complement(21455..23212)
                     /gene="ynbC"
                     /locus_tag="ACHK3A_001382"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO8739493.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLASSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIISDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALVFEKMQ
                     SFISHLYANKSQKLDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDIFLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRGN"
     gene            complement(23228..24124)
                     /gene="ynbB"
                     /locus_tag="ACHK3A_001383"
     CDS             complement(23228..24124)
                     /gene="ynbB"
                     /locus_tag="ACHK3A_001383"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO8739494.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWVTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGLNC
                     FELFLLFIPLFGFLILATWRVFVGEPSGFLHTVSAIFCGWIMTVFALSHAAWLLMLPT
                     TNIQGEALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     VSLIIGPLLTPLDTLQALLAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(24124..24729)
                     /gene="ynbA"
                     /locus_tag="ACHK3A_001384"
     CDS             complement(24124..24729)
                     /gene="ynbA"
                     /locus_tag="ACHK3A_001384"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO8739495.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLHKRQVSANQITLAALALSLLTGL
                     LLALVPYHGMFLLLPIVLFVRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWVL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMNAEK"
     gene            complement(24898..26243)
                     /locus_tag="ACHK3A_001385"
                     /pseudo
     CDS             complement(24898..26243)
                     /locus_tag="ACHK3A_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(26307..27167)
                     /gene="pdxI"
                     /locus_tag="ACHK3A_001386"
     CDS             complement(26307..27167)
                     /gene="pdxI"
                     /locus_tag="ACHK3A_001386"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO8739496.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLGALDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(27398..27988)
                     /gene="paaY"
                     /locus_tag="ACHK3A_001387"
     CDS             complement(27398..27988)
                     /gene="paaY"
                     /locus_tag="ACHK3A_001387"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO8739497.1"
                     /translation="MPIYQIDGLTQFVPEESFVHPTAVLIGDVILGKGVYVGPNTSLR
                     GDFGRIVVKDGANIQDNCVMHGFPDQDTVVEEDGHIGHSAILHGCIIHRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRSKQTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            complement(27970..28122)
                     /locus_tag="ACHK3A_001388"
                     /pseudo
     CDS             complement(27970..28122)
                     /locus_tag="ACHK3A_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
     gene            28123..28329
                     /locus_tag="ACHK3A_001389"
                     /pseudo
     CDS             28123..28329
                     /locus_tag="ACHK3A_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28501..28827)
                     /gene="ydbL"
                     /locus_tag="ACHK3A_001390"
     CDS             complement(28501..28827)
                     /gene="ydbL"
                     /locus_tag="ACHK3A_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO8739498.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(28835..29020)
                     /gene="ynbE"
                     /locus_tag="ACHK3A_001391"
     CDS             complement(28835..29020)
                     /gene="ynbE"
                     /locus_tag="ACHK3A_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO8739499.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(29017..31656)
                     /gene="ydbH"
                     /locus_tag="ACHK3A_001392"
     CDS             complement(29017..31656)
                     /gene="ydbH"
                     /locus_tag="ACHK3A_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO8739500.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVVARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGHLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWSE
                     DEPLLLTDVSVDVLGGNVLMKQLRMPQHDSALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNAHLPGNDCPQGKECEDKQ"
     gene            31864..32853
                     /gene="ldhA"
                     /locus_tag="ACHK3A_001393"
     CDS             31864..32853
                     /gene="ldhA"
                     /locus_tag="ACHK3A_001393"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO8739501.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFEQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            32964..33386
                     /gene="hslJ"
                     /locus_tag="ACHK3A_001394"
     CDS             32964..33386
                     /gene="hslJ"
                     /locus_tag="ACHK3A_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO8739502.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(33383..33649)
                     /gene="ydbJ"
                     /locus_tag="ACHK3A_001395"
     CDS             complement(33383..33649)
                     /gene="ydbJ"
                     /locus_tag="ACHK3A_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO8739503.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTTIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            33923..37447
                     /gene="nifJ"
                     /locus_tag="ACHK3A_001396"
     CDS             33923..37447
                     /gene="nifJ"
                     /locus_tag="ACHK3A_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO8739504.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPDSVRTVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSVDEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLKDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            37813..38946
                     /gene="ompN"
                     /locus_tag="ACHK3A_001397"
     CDS             37813..38946
                     /gene="ompN"
                     /locus_tag="ACHK3A_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO8739505.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            39087..39521
                     /gene="uspF"
                     /locus_tag="ACHK3A_001398"
     CDS             39087..39521
                     /gene="uspF"
                     /locus_tag="ACHK3A_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO8739506.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            39697..40632
                     /gene="ttcA"
                     /locus_tag="ACHK3A_001399"
     CDS             39697..40632
                     /gene="ttcA"
                     /locus_tag="ACHK3A_001399"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO8739507.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(40761..42134)
                     /gene="dbpA"
                     /locus_tag="ACHK3A_001400"
     CDS             complement(40761..42134)
                     /gene="dbpA"
                     /locus_tag="ACHK3A_001400"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO8739508.1"
                     /translation="MSAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPTILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRMLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(42164..42337)
                     /gene="ynaL"
                     /locus_tag="ACHK3A_001401"
     CDS             complement(42164..42337)
                     /gene="ynaL"
                     /locus_tag="ACHK3A_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO8739509.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(42612..43595)
                     /gene="zntB"
                     /locus_tag="ACHK3A_001402"
     CDS             complement(42612..43595)
                     /gene="zntB"
                     /locus_tag="ACHK3A_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO8739510.1"
                     /translation="MEAIKGADVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(43609..43656)
                     /gene="ydaN"
                     /locus_tag="ACHK3A_001403"
     CDS             complement(43609..43656)
                     /gene="ydaN"
                     /locus_tag="ACHK3A_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO8739511.1"
                     /translation="MLRNRARRLDYKIMR"
BASE COUNT        11338 a        11311 c        10226 g        10834 t
ORIGIN      
        1 atgccgaaaa aggggctgcc gaaaaagggg ctaagtcaat catcagtagt taaatttcag
       61 taagttaagc tgctaaaaaa tttcgactac tgtgattaat ttcacatcca ttcgataaaa
      121 aatatgctta ccgaaaagaa gaacagtaaa ccatctcagc gtggggaatt ttcacgcgcc
      181 ttgttcctag cgggtcattt tttacgtgcc agcatcgtgg cgaaacgcag tttaatacga
      241 ttaccgttgg cgtcggtgcg gtgcagctcg ccgacgtctt cattgtattt caccatctcc
      301 cagccttcgt aatatcgacg taattccccc tctttgaagg caaacgggaa gccgacggta
      361 catggataat cagcggtatc catcgccgcc acaatcaggt tgtaaccgcc aggtttagtg
      421 caacgttgca tattggcaat cagccctggg atggttttag cctcaaggaa catcagcacc
      481 acagtcgaaa gaataaaatc atactgtcca tcaaatgtga ggttattcag atcaacgacc
      541 cgggtgtgta aattctccag attttcaatg gatttaatcc gctcgacgtt ggcgatactc
      601 atggcatttt tatcccatgc gtcaacatca taaccattgg ctgccaggta aagactgtta
      661 cgaccattgc cacagcccag atccagcgtt ttacccggtt taaccacttt caccgcttcc
      721 agcacttcag agtgtgtgcg agttaattca tatttatcag taaaatagtt ttcgtcacga
      781 atgatcattc tttgtcctct gctttcatta aaacggcgcg ctcggttctg attaacaatt
      841 ttccctgcat cagaagcgca aaagtacgga tgagcagtat tgcaataata aaattggtaa
      901 agataaacag cggcaccgcc agcgtatgga aaaatccatt atcgctgcca ctccccagat
      961 gcaaaccggt ggttgccagt gcagatacgc cgaacgagaa actccagaat gaagcattaa
     1021 atggctggga gagataccat ggcatcagac gtagcataaa cagcagttgc agcagtccat
     1081 aaccgaaaag cattttcgcc agcgtgtcac cctcgccgcc gttgacgctc agccaggcac
     1141 tacaggccac cagcgcagga gcgagctgaa tgccgagtga tgtccgcagc gccgtgggta
     1201 attctcccga actgcgcaga cgctgcaaga tcacaggttc aaggcttagc catgagaaaa
     1261 cgcctgcgcc taaaaacacc agaccggcgt cggtgtagcc caacgcacca caggccattg
     1321 cgctgataaa gttgttggca actgtcggca gatacagtcc aggcgtggta gcttcttcag
     1381 ggtgagatcc gcgccataat cccgcagttt gccaggcggc ataagccaac tgaaccacga
     1441 cgccaaaact gaacaggcac accgccagtg ggcgaaacca cggaacaaaa ccaatcgcca
     1501 ccagcatcgt cgttgccgga aacaaactca caaaactgct cagcactgga tggcgcactt
     1561 ccgccagcac gctatgcgga aagcgtatga gtcgggtaat aaatgcgcta gttaataatc
     1621 cccagatgat catcgccaga atcaccagcc catcccctaa ccagtggctg acctgccaaa
     1681 cctggctggc atagcgccag gcaaatccca tccctatcgt ccccaacaca ataccaaagt
     1741 agcctgccgg caaattgagc actttatcgc tctgcatctt cttactcatt ttgtttaatt
     1801 tataaagtat atttgaaatg cattttatgg aattttctcg agcatagcca gagccgcaga
     1861 atttgctacg gttatagtaa gtcacctgtg ggaaaacgct atgcgcaaat atcgtctaag
     1921 tgaagaacag cgagccttta gttatcagga tgatggcact aaaaaaagtg tgttattacg
     1981 gcagattatc gccatgagcg attttaacga tgtgatagca ggaaccgctg gcggctggat
     2041 cgattgcgaa acggtgttag cacaggaagg aaattgctgg atttacgacc agaatgctat
     2101 tgcgttcggc ggagcggtga tttccggtaa tacgcgcatc accggcacca gcgtactgtg
     2161 gggagaggtt tacgcaacgg ataacgtctg gatcgataat agtgaaatca gtcagggtgc
     2221 atatataagt gacagtgtca ccattcgtga ctcattagtt tgcgggcaat gtagaatatt
     2281 tggtcacgcc ttaattgatc aacattctat gatcgtcgcc gcacaaggct taacgcccga
     2341 ccatcaactc ctgctacaaa tttatgatcg cgcaagggtt agcgcctcgc gtattgttca
     2401 ccaggcacaa atttatggcg atgccgtcgt ccggtatgct tttattgaac atcgcgccga
     2461 agtttttgat tttgccagcg ttgaaggtaa tgaagagaac aacgtctggc tatgcgattg
     2521 tgctaaagtc tacggtcacg cgcaggtgaa agctggcata gaagaagatg ccattccgac
     2581 gattcattac agttcgcagg tggcggaata tgccattgtg gaaggtaatt gtgtgttaaa
     2641 acatcatgtc ttgattggcg gaaatgccgt ggtacgtggc ggaccgattc tgctcgacga
     2701 gcacgttgta atacaaggtg agagccgtat tacaggtgcg gtgataattg aaaaacatgt
     2761 ggagttaacc gaccacgccg tcgttgaagc ctttgatggc gacacggttc atgtccgtgg
     2821 tccaaaagtc attaacggcg aggagcgcat tacgcgaacg ccgctggcag ggttattgtg
     2881 aatcgataat acgcgcgtat aagttcacat catcataggc atcattcagg aactcagcct
     2941 gtttcagaca accttcaagg ataaaaccat tgcgcaaagc gacctggttg ctttgcgcat
     3001 tgtccacccg acatttgatc acgaagcgtc taagttcacc agactgagcg taatgatgaa
     3061 tcaatgcctg cagcgcctga gaaatgatcc cctgcccctg atgagattcg tccagccagt
     3121 agccgatttc agcggtttta ttcagtggtt caatacgatt aaacgagata acgccgataa
     3181 gttcatcttc tttgaaaatc atgaacattt tggcatagcc gcgttgatgc aacatcacat
     3241 taccctgcac cgtttttcgc gtgtcctctt cactttgaac aaactgcggc cagtttagcg
     3301 actgctgtaa ccaggttcta tttttacaga ttaactgata aagaggtgtg acatgacttt
     3361 ctgcaacagc atgtaattca agtgattcac ttacttttat cgtttcagtc atcttatctc
     3421 cagtcaatcc atgcagttgt taaaaaagcc gcctggctgg cggcttagga aagtttacac
     3481 ctctttgacg ctcacctgct gcaggatttt gagcatctca tcttttaact gtagcttttg
     3541 ttttttcatg cggaccactt ccgcattgta ccctcgaccg tcggaacctt cctttctggc
     3601 aatttcatga tcaagtttat tgtgtttatc gaacaaggac ataaagcgag gattttcgtt
     3661 tttcagacgg gatattaaat ctcggtattc tggaaacata cgcactccct gttagtggct
     3721 gacaaggtgt atctgaaata cacaccatca gattactcat ttaacttagg gaaaaatgcg
     3781 agcgggatcg tgtttaacca gacggcacct cccgaagaag atgccgaaga aaaaacgatt
     3841 aactcatcac gcggtcatca acatactgac gtttatccgg cgctggcggg aaatactgat
     3901 acagccatgt ttcactgata gcgtcgccct gacaacgcag atacatccgc atatcgaccg
     3961 gatcagtgga gtccgaagtc ggataccagt caaactgaat acgataacca tcaatgggtt
     4021 caatatagag aatttcgatt tgtttcgctt ccccactgga aagcgtaatc accggctcaa
     4081 tgccttttgg cgcggcagct ttcagatcac caccaacgaa atcgacggca aaacgtcgcg
     4141 cccatttttc gggatagtgt tcacctggcg cccaaccttc cgggaaaccg cccatgccgg
     4201 tacgcgtcgc cataacgcgc gctaatgggc aatgaacagg cggttgcgca ctccagtaca
     4261 gacgatactg gaatgcaaac tcatcacccg cttttaccgc tttttctggc tgccagaagc
     4321 agacaatgtt atccagcgtt tcgcccgttg ttgggatttc catcaggccg atggtgccct
     4381 taccccactt gttacgcggc tccacccaca gacttgggcg tttgttatac cagcccataa
     4441 tgtcctgata atgggagaaa tcacgatcca gttgcaataa accaaaccct ttcgggttgt
     4501 tgtcggtgta agcattgaac tgcaattttt gcggattatt cagcggacga caaatccact
     4561 cgccattgcc ccgccacatg gacaaacgat cagagtcatg gatttgcgga tgaatggtgt
     4621 cgcacatccg acgttcatta gtaccgcagc tgaacatgct ggtcatcggc gcaatgccca
     4681 gctgtttaat gtctttgcgc gcatacaggt gattttccac atccataatc acctgacttt
     4741 tctcacaatg gatagtgaac ttataggcac cagtaatgct ggcgctatcg agcaacgcat
     4801 aaacggtaaa ggtggttgcc cccggtttta ccgtatcaaa ccagaagtca gtaaagtcgg
     4861 ggaactcttc tttactgtcg gtgtaagtgt cgatcgccag gccgcgggcc gacaaaccgt
     4921 attgatatgt atcatcaacc gcgcggaaat aactcgcgcc gaggaatgat acgacatcac
     4981 ggcgcgccag ttcgggggct ttaaacacgc gaaaaccggc aaaaccgaga tcactttgtc
     5041 cttctaattg tttggtatca acacccgcat cgttgtattt gaacaactcc gggcgaaagt
     5101 gaatttcacg cgccagatgt gttgctggat ctacagaaaa catacgaacg cggcgacgga
     5161 atcccattcc catatggaag aactgagcgt ccagttgacg gttctcaacg ttatgccaga
     5221 gcgatttttc ggcgtcgtat tgaatactgt tataagcctg cggcgtcatt gtcgccagag
     5281 tgtcaggtaa cggacgcggc gcaccacgcc acgctgtttg cgctaagtcg tgcgccattg
     5341 actgtagaat ggagaagtca aaacgttggg tttgcccgtc ggcaatatca gaatctgccg
     5401 cgaatgccgc ctgagaaaaa agagaagcaa tgccgctggt accgcacacg gcggccatag
     5461 ccattgaacc tttaataaat cgtctacgat ccatacctga aagtgagtcc ttctggtgtt
     5521 gtgaatggtc ccgcgctgat cgttttcagc ggcaagaaag tgcttattta tgaccgctca
     5581 ctctagacaa tatttattaa taatccaatt gttgaactga gagaatatgc ataaatcggc
     5641 aattttttta tgtcgatggg atgagcctga ttcgtttgta aaatcaggca aatttttgca
     5701 aaggcggatg gtcagaattt aaagcaaacc acagcgacgt ttactgcgtt cttccgcttc
     5761 ctgataaagc tggaactcat ccaaaaccgg gcagcccaac gcctgctcaa atgcttcgcg
     5821 actggcatta ccgtgactgc gtgcctgcgt ttcattaccc agatttgact ggaagatccc
     5881 cgccgcgcta acaggcaaga aatcttcata ggtaatgggt tgcgccacta cccaaccacg
     5941 ttcaattaag ggctgtggat cgtctccggg atgaatcgcc tgacgatgcg tctcacctga
     6001 aggcgtcaga cggtaccgga accatgccaa cccttgctga cgcattaaaa actcactgtc
     6061 aggaaaagtg cggaaggttt cctgtaaatg catttggtga gtgagattat cctgcccggt
     6121 tccagcgttc cgcagtagat catcatacag ttgtcgtcct ttcggcgtta atgccacgcc
     6181 acgctgttct atttcaccaa agcgcgcggt atgcgtgccc tgtttctgcc ctgcaaacaa
     6241 caccgtctct tccagtgctt taaagctagt ctggcgtagt aaaatcggta cctcgcggcg
     6301 cggcggccct tcgatcagaa ttttgggttc aattccgcat tcaggcatca tcgactgcac
     6361 ccggtcaata tccagcgtgc gtggcgtcag gtggttgata tggcatccag gaaaacagac
     6421 cacatcagca atcaaccgat gttcgttgtg caaggcgcga taggtttctt catctaccgt
     6481 tgctgactgg tgccaacgaa acgtttccag ggcttcctgc acaaactcct gtgcctgtgt
     6541 ttcgttaaaa ccgccccgct gctcatattc ctctaacagt tgtcgacaac gtggggtgaa
     6601 gatatcgcgc tgacgcagaa tctccgccgc tttctggcgc aaaatttcgt tctcgataag
     6661 ctcaaggcgg agtaatgaag taaaaacgcg gaagggatta cgcgccagag aagcatcatc
     6721 aatgggccga aatgctgtgg aatgtaccgg cacccccgcc tgcgagagat cgtaatagct
     6781 caccgggtac atccccataa tggcaaacat ccgccgaaga gtagcaagct cttgtgcagt
     6841 cccaacacga atcgccccat gacgttcaac attcagtcgc gccagctcgt ctgcatttac
     6901 cattttttcg tgcagttgag gattgttttc cagcacagcc agattcacat cagctaccag
     6961 ttccagcagc gtaccgtact gcggaacttc ttgctggtac atggctgaca ttgcctgcga
     7021 aaactgttcc cgaatctcat ccgccgtgat gctgttcgcc ataatgtctg cctccagtgg
     7081 tcagtaatct ggagtgtagg taaccgcatt cactctggcg ggaagaattt gcaaactgtg
     7141 atctcgccgc gaaaacatca atattatcca tttcgctgta acataattgc tttaattgtt
     7201 aataatattt tgcaatcaag ttatcataat caaacaactt cacttgtcag cgacaccgct
     7261 tcgtttttaa catcgcttat ggaaaaaaat agtctgttta gtcagcgcat ccgtttgcgc
     7321 caccttcata cattcgtagc tgtcgcacaa caaggaactt tggggcgcgc ggctgaaacc
     7381 cttaatttga gtcaacctgc gctctctaag acattgaatg aactggagca gctgacgggc
     7441 gctcgcttgt ttgagcgtgg tcgtcagggg gcgcaactta ccttacccgg cgaacaattt
     7501 ttaacgcatg cagtcagagt gcttgacgcc atcaacactg ctggacagtc gcttcatcgt
     7561 aaagaaggtc ttaataatga tgtcgtcagg gttggtgcac taccgactgc ggcactgggg
     7621 atattacctt cggttatagg tcagtttcat cagcaacaaa aagagacgac cttgcaagtt
     7681 gcgacaatga gtaaccctat gattcttgcg ggtttaaaaa ccggggaaat cgatatcggc
     7741 attggtcgga tgtcagatcc tgaactgatg accggactta attacgaact gctgtttctt
     7801 gaatcgctga agctggttgt ccgccctaat catccgctac ttcaggagaa cgtaacgcta
     7861 agccgggtgc tggaatggcc ggtcgttgta tcaccagaag gcactgcgcc acgccagcat
     7921 tcagatgcat tagtgcagag ccagggatgt aaaattcctt cgggttgtat cgaaacgctg
     7981 tctgcttcgc tatctcgtca acttacggtt gaatacgatt acgtgtggtt tgtcccttct
     8041 ggcgctgtaa aagacgacct gcgtcatgcc acgctggtgg ccctgcctgt tccgggtcat
     8101 ggtgcaggcg aaccgattgg aatactgacc cgcgtagatg cgacgttctc ttctggttgc
     8161 cagttgatga ttaacgctat tcgaaaatca atgccgttct gaaaggtgaa gggatctgtc
     8221 gatccctcct ttaacatttt cacactgtag cgaaactaac tggttcacca gctccgcgag
     8281 gttctgccga cacagaatgt ttgttcagac ggaatacgtc caccgcctca gttaatcgtg
     8341 ccgcctgttc ttcaagggac actgccgctg ctgaggcctc ttctaccaga gaagcattct
     8401 gttgcgtcac cttatccatt tcagatatcg cctggctaac ctgcgttata cccctacttt
     8461 gctcatccga ggcggcggca atttcctgca tgatatgtgt gacactcgcg acggcatcaa
     8521 caatagtgct catggttttc ccggccgtgg ccacctcatc cgatcccagg tcaattaacc
     8581 tgactgattc actgatcaag ccttcaatct ctttcgccgc ctgagcgctg cggcttgcga
     8641 gtgtccgtac ttcgctggcg acaacggcaa atccacgccc ttgctcaccc gctcgcgcgg
     8701 cttcaacggc agcattcagt gccagaatat tcgtctggaa agcaatactg ttgatgacgg
     8761 cggtgatctc agaaattttc ttcgaactcg tggagatagc gcccatcgtt tttactacac
     8821 cggaaaccgt ctgcccgcca tcgctggctt taatagaagc ctcttgcgcc agtttgctgg
     8881 catgatgcgc gttatccgca ttctgtttca ccgtcgcagt gagttgctcc atgctggcgg
     8941 cagtttgttc gattgccgcc gcttgttctt cggtgcgaga tgaaaggtcc gcattgccag
     9001 cggaaatttc gctggtgcca cgataaatct cttccgcacc ctgtcgaaca gtccctactg
     9061 tcatccccag tgaatgctgc atttgctgta aatgacgact taagcgaccg atttcattac
     9121 gacccgccgg ttcatcattc atcgtcagat cgccactggc gattttttca atccgttgtg
     9181 cggcatgttg cagtggacga atgacgatcc gacgtagcac cataaatgtt atcagcgtca
     9241 tgaccagggc aagcacaaac gcgccaatca tgaacatccc acccaggcgg gtgcgctgat
     9301 gggccagttc cgccagttgg ttggctctgg tgctacgtat cttaacggct ttgctcaaaa
     9361 tatcggtata agcattatcc agcggtcgga tctgctcact ttcatgattg ataatcgctt
     9421 caaacatgcc atttttggca tatttcaaca taggttgcat acccgtgata taagcctgga
     9481 agcgttgatt taattcagtg tcgagggctt catcagcagg tgttttcacc gaccgattct
     9541 gataagcacg ataaccttgc tgcgactgtt taatctccga ttcggcttgc gcaatatttc
     9601 gcttcattgc ttccatttcc gcaatacgac tcgctgcccc ggcctgaatc atattgatcc
     9661 gggctgaacg caaaaaatct gaactgttcg ctaacccggt ccgaatctca atctccgcag
     9721 taacatcacg ctgatcgcga tcggcctgcc agaggaaata gccagccaga gcagaactta
     9781 atgcgaatag aactaagata ccgccaagaa tgcaggcaaa taacggaacc agcctgatgt
     9841 gatgcaaaaa acctaatcgc tgtgagggag ttgtattcat taccgttgtc tctcgtccag
     9901 gtttactcct gatagaaagt catcggcact tgacggtaat tacttatgac caaaaacctc
     9961 tttgcgtcac atttttacaa caattatggc agagaagaat aatgccattg gaatgaagag
    10021 agtaacgaat gattaaactg gctaacccga atgacgaaaa attctggaaa acagcacgca
    10081 atttcgtcag gtgattattt tcgtccctaa ggaaaccaca taggatgctc acgggtgcct
    10141 gagactttct gttttcaggc taaccgggaa tcagaaagac gaaggccccg accgatattt
    10201 ctatcaaccg aggccaacga atgaacacag caattcagat cttagcctct tacccgccat
    10261 ccggcaagga gaaaggctat gaagcgcaac cctctggtgg tgtgtctgct cattatctgc
    10321 attacgattc tgacattcac actcctgacc cgacaaacgc tctacgaact gcggttccgg
    10381 gacggtgata aggaggttgc tgcgctcatg gcctgcacgt ccaggtaagg gcaagcgcgg
    10441 ggattttccc cgcgcatctg actagcagat tcattttctc atggcacccc ttttacactt
    10501 cagttaatgc atgttttttt cgatgcacta atagaaccat cattacaaac aaatttgccg
    10561 cctgccgtac agtgtgagat cccccctttc gaaccagaac agggtttgcg cccggcatcg
    10621 gcataaaagg aaacaagcgt tcctataacc aagataagtg ccagtttttt catataacct
    10681 ccatggttta tcgaatctta tgttcgattt taacgttata cgctggttta ttttgatacc
    10741 gttgatgaga tcaaagatcg ggtgaattga ttaaaaataa gccagatgga cgtatgggca
    10801 aggattatgg ttttatttgt catacaaata aatataatag gcgcttccga tgtagacccg
    10861 tattcttcgc ctgtaccacg ggtcggtttt agtacaggcg ttttctttaa atatccttca
    10921 ggaacgttta cgcggcatca tgcgtagaag tgtgttgtcc ttccagaaat agtggtgtgc
    10981 cagtgcagcc gcagcgtgca acccgatgac aaaatatccc agattcgcca gcatttcatg
    11041 ccacgacttt aagctatcta cccgttcgaa attggcctct gaagcgtaag gcatcgtcaa
    11101 accaaacgca aaccacgggt tgccccggtt atacatcatc accaaaccaa tcactggcag
    11161 cgcaataaac aggagataaa tcaccaaatg gcccaaatgc gccagtcccg tcatcatcgg
    11221 ttttggctta ggtataatcg gcggggttgg gtatttcagt cttaacaaaa gacgaaccac
    11281 catcagtacg agaattgaga tgccacagga aacatgaatc atgttgatga ggggccgatc
    11341 gctacgtggg aagaaaccac gaaactccat tgcgcaatac gctacgataa ccagcaaaaa
    11401 gaccagccag tgaatgctga tttgtaacct tgagtattta tttcccataa catttcctgc
    11461 tttaacataa tttgccgtta acataacggg cttttcttaa aatttcatta aatattgttc
    11521 acccgttttc agtacgttac gtttcactgc aagaatgtag attgcccatc ccgccatcct
    11581 ggtctaagcc tggaaaggat caattttcat ccgaacgttc ctgacaggag aagaccatga
    11641 gtaaagttgg tattaatggt tttggtcgta tcggtcgact ggtgttgcgt cgattacttg
    11701 aagtcaaaag caacatagac gttgtcgcta ttaatgatct cacttcccca aaaattctcg
    11761 cctacctgct gaaacatgat tcaaactacg gaccgttccc ctggagcgtt gattttacgg
    11821 aagattcact tatcgttgat ggaaaaagca tcgcggttta cgccgaaaaa gaggctaaaa
    11881 atattccgtg gaaagcgaaa ggggcagaaa ttattgtcga atgtactggc ttttatacct
    11941 ccgccgagaa atcgcaggcg catcttgatg ctggtgcgaa gaaggtgttg atttccgccc
    12001 ctgccggtga aatgaaaact atcgtttata acgtcaatga cgacactctg gatggcaacg
    12061 acaccattgt ttccgtggcg tcatgcacca ctaactgcct tgcgccgatg gccaaagcct
    12121 tgcatgacag tttcgggata gaagtcggca cgatgacgac cattcatgcc tataccggca
    12181 cccagtcact ggtggatggt ccgcgtggga aggatttacg agcttcacgc gcagcggcag
    12241 aaaatatcat tccccacact acaggtgcgg caaaagccat tggtctggtg atcccggaac
    12301 tgagcggcaa actgaaaggt catgcgcaac gcgtgccggt gaaaacaggt tcggtcactg
    12361 agctggtgtc cattctcgca aaaaaagtga ctgccgaaga ggtgaataac gcacttaaac
    12421 aagcaaccac caataacgag tcatttggtt ataccgatga agaaatagtc tcttccgata
    12481 tcattggcag ccatttcggg tcggtgtttg atgccacgca aacggaaatt accgccgtgg
    12541 gcgatttaca actggtgaaa acggtcgcct ggtacgataa cgaatatggc ttcgtcacac
    12601 aactcattcg caccctcgaa aaattcgcta aactctgacg cacacaggcg gaggaaaaac
    12661 ctccgcctct tccactcatt aagactgtaa ataaaccacc tgggtctgca gatattcatg
    12721 caagccatgt ttgccatctg cgccgccaat accagattta cgccatccgg cgtggaatcc
    12781 ttgcatagct tcgaagtttt cacggttgat gtaagtttca ccaaacttca gccctttaat
    12841 ggctttcatc gcgacgttca gattttgggt atagattgat gaggtcaggc cgtaatcact
    12901 atcattcgcc attgagatag cctcttccag cgtgtcaaat gcgaccaccg gcagaaccgg
    12961 gccaaaggtt tcctcatgca taatcgacat ttcctggcga acatccagca gcaatgtcgg
    13021 cggataataa tatcctttcc cctctaccgc tttgccaccc aacgccactc tcgccccttc
    13081 ttctactgcg cgcgccactt tttgctcgac cctttccagc gccgcggcgt taatcaacgg
    13141 ccccatcgca atgtcgttgc gttcagcggg gttaccaaat tgaaccgcct gcatcgcttc
    13201 acccagccga ttgacaaact gatcataaat gcctttctgt acataaacac gttctgcaca
    13261 gttacacact tgcccactat taatgacgcg tgaatcaacg atggctttga ctgccagttc
    13321 aagatcggca tcgtccatta cgatagccgg tgctttaccc cccaattcca ggcacacttt
    13381 ggtgatgttt ttcgccgcag tcgccataat cttttcacct gcagagacgc tgcctgtcat
    13441 actgaccatt gcgacctttg ggttacccgc cagttcttgc ccaacggttt caccacgccc
    13501 cagaacaagg ttaaacacgc cgcgcggaag gcctatttca tcgacaattt tggcgaatgc
    13561 aatcgcattg tttggcgtaa attcactggg tttaatgacg atggtattac cggtcaaaag
    13621 agcgggagcc attttgcggg caatgaggaa gaacgggaag ttccacggca gaatgccggt
    13681 agtcacgcca agcgcacgtt taaacagaag aatattttct cctggacgat cgctttgaat
    13741 aatctcgcct tcataacgcc gtgcccactc cgccatgtaa tcgatatagt ccgcagtaaa
    13801 agccacttcg acttcagcca gctgctggat cttaccccct tcttcaacaa tcagcgtact
    13861 gatttcactg gcgcgttcgc ggatcccggc ggagattttg cgcaaccaac tggcgcgttc
    13921 aatagcaggc aacgcttccc attctggttg tgcacgttct gctgcatcga ttgccttacg
    13981 ggcatcctcg gcctgaccat cgggaatgcg ggaaatgaca gcctctgtag cagggtttac
    14041 cacatcaatc catgcgtctc cacgccaggt aacaaactgt ccatcaatat acataggatg
    14101 ttgaacgggt actgacatga gcgactcctg tgatttatat gtttgttttt ggtaaatgta
    14161 ttgttaatta acatcaatca tcatagagac ggcatcatga aaggacaatt ctgtgaaggg
    14221 cttcataaaa acaagtataa aaggaataag aggcagaaat aaaaggcagg agttatgccc
    14281 gtttgcaaac aagtggttca caaacgggca atttttaacg taacgagcga ctttccatcg
    14341 cctcgattag caccgcatcg tgtttcagcg tttgccatgc atggatgact tctgccggaa
    14401 aatcaacgtg aacgataaaa tctcgcccgc ggggaccgta gccatcgcta tcatcgcgta
    14461 aacgcggcag ctcgccagtg agtagtgaga gataacgctc aaccggccat aatccttgca
    14521 acggattaac aaagattaca ctgtctgcgt tatttcctaa ctgaggaacg aatccgggat
    14581 aacttaacca gcgtggtgca tcgggattgt ccccagtcat acggcggaac gtcgccatcg
    14641 tgcgccgctg catggtgggg tcctgaacaa cgatagccgt atgaactcgt tctacggcct
    14701 gattcaatag cgcgatgcta aagcgtgcgt tttcaccgca gtttgttgac tggtcttcaa
    14761 tccagatttt ttcatgcgga atgtgccaga actgatgagc gatatccgcc aggatggtcg
    14821 cttctgctct gccagtggtg cggatagtgt tgtagtgcgg atgctgtgcg atggcgctat
    14881 acaaaaaagt tgtcgagtga ccgataccac cactaatcag tagaggaatt tgctgatcgc
    14941 gagcaatctt acatgccgcg tcgatagtcg gcataaccgc attgcctgca aggatcacgc
    15001 aatcggcctg atacggcatc tcaccggaga aatcatcctg cgccagccac tgaccgataa
    15061 catttatggc atctatggtt gccggcgaaa gcgtcggaaa cggggtaatg ttcatggttc
    15121 ctccttatcg ttcccttaag aatactgcgt gagataatag tgagaagcgg agcactctga
    15181 aaggaggaag tcgtatttcc ttaaccgcca ctccgcaagc ctgacatatc ttttaaggaa
    15241 atgctgtaga taaacattat tcatcttgca gctgagaatt gtatttttct gaattcctga
    15301 atttttgccc ttaccgtttt agaattcata aaaaacccgg cgctaaatgc ttaccgggtt
    15361 ttagatttat caggcaaata gccgcgttaa ccgctaatct gttccatcgc ctgcaaaata
    15421 cgcttatctg aaatcggata aggcgtacca agttgttgag cgaagtaact aacgcgcaac
    15481 tcttctatca tccaacggat ctctttcacg tcttcatcct cacgacgtgc gggcggcagt
    15541 ttgttgatcc attgctgcca cgcctgctgg acattttcga ctttcagcat ctgagcacgg
    15601 tcacgatgcg gatcaaccgc cagtttttcc agtcgttttt caatcgcctg caaataacgc
    15661 agcgtgtcgc ccagccgttt aaaaccgtta ccagtgacaa aaccgcgata caccagcccg
    15721 cccatctgcg ctttgatgtc agaaagcccc agcgccatag tcatatccac ccgccctttc
    15781 aggcgtttgt tgatattgaa cactgccgta aggatttgct cgacctgctt cgcaatatcc
    15841 accaccgtat cgttcagttc agcgcggact ttctcatgca gcgcagcaaa gccttcttcc
    15901 gtccagaccg ggcctccatt ggcgtcgatc agttgatcca ccccgcagga gatacagtcg
    15961 tcgatcagct ccagcacttt gccatacggg ttaaagtaca gccccagctt ggctttgttc
    16021 ggtaactttt catgcaaata cttgatcggc gatggaatat tcagcagcag taaacgacga
    16081 agaccgttcc acatcgcttg cttttgttcc agcgggttat caaacagttt gatcgccacg
    16141 ctgtcgcgct catccaccaa cgctggccac gccttcactt tgtagttgcc acgcttctgt
    16201 tcgtagcttt ccggcagttg cccaaagctc cagatatgta agccgctctg ctcgataccg
    16261 tcatccgcca ccgcagacag cgtttcctgc actttgcctt tcagagcatc tttcagatcc
    16321 tgtaatgagc gcccttcttt tagcttcttg tttttgtcat ccaccacacg gaaggtgatt
    16381 ttcaggtgat cgggcacctg atcccagtgc cagtcttcgc ggtcaacggt gacgccagtc
    16441 atccgccgta gctctcgttc aagactgtcg agcaacgaca attccaacgg tgtgacgcgg
    16501 cctagaaacg cttcggcgta gtttggcgcg ggcacaaaat tacggcgtac tggtttcggc
    16561 aacgatttaa tcagagcaat caccagttcg cggcgcagac cggggatttg ccattcaaac
    16621 ccgctctctt ctacctgatt aagcagcggc agcggaatat gcacggtcac accgtccgcg
    16681 tccgcgcctg gctcaaactg atagctcaaa cgcagcttga gattgccctg atgccagaag
    16741 ttcggataat ccagcttgct gattttttcc gccccttctt tgatcaacat gctcttttca
    16801 aagttgagca aatcaggcgt ttcgcggctg acttttttcc accagctatc gaagtgacga
    16861 gcggagatta catcgtggct gatgcgctgg tcgtagaact caaacagcgt ttcgtcatca
    16921 accagaatat cgcggcgacg tgatttgtgt tccagctctt ccacttcggc ccgcagtttc
    16981 aggttttcac ggaagaatgc gtgacgcgtc tgccagtcac cttccaccag agcgtggcga
    17041 ataaagagtt cacgacataa cgccggatcg atctggctgt agttgacctt acgcgcagca
    17101 acaatcggca aaccataaac agtgactttt tccgttgcca tcaccgcgcc ctgcgcccgt
    17161 tcccagtgcg gttcgctgta ggtgcgttta atcaaatgct gagcaacggg ttctacccat
    17221 tcagggtcga tacgcgcagc aatgcgcccc cacaggcggc tggtttctac cagttccgcc
    17281 accattaccc atttcggcgg ctttttgaat aaaccagaac cgggaaagat ggaaaaacgc
    17341 gcgttacgtg cgccggtata ttcttgttta tcggcatctt tcatgccgat atgggaaagc
    17401 aaacccgtca gcaacgcaat gtgaatttcg cgatactccg ccggttcgct gttaaccgga
    17461 atgccaagtt ctttcaccac ctggcgcaac tgggtgtaga tatcctgcca ttcgcgcacg
    17521 cgcagatagt tgagataatc ggtacgacac aggcgacgga aggcgttaga agaaagcgcc
    17581 ttttgctgct cgccgagata attccacaga tttacaaacg cgaggaagtc ggattctttg
    17641 tcgtggaagc ggcgatgttt ttcgtctgat gcctgctgtt tatccatcgg acgctcgcgc
    17701 ggatcctgaa tggagagcgc ggacgtgata atcatcgcct cacgcacgca gccatgtttt
    17761 tgcgcttcca gcaccatacg cgccagacga ggatcgacag gcaattgcga aagctggcga
    17821 ccgagcggcg tcagtttata ggcgctggcc tgttcatcag tggtgatcgc gcccagctct
    17881 tcgagcagac gcacgccatc ctggatatta cgtttatccg gtgcttcgac aaatgggaac
    17941 gcggcgatat cgcccaaccc cagcgcggtc atctgcaaaa taaccgaggc caggttagta
    18001 cgcagaatct ccggatcggt aaactccggg cgcgagagga aatcgtcttc ggaatagaga
    18061 cgaatacaga tcccttcgga cacacgacca cagcggcctt tacgctgatt ggcagacgcc
    18121 tgagaaatcg gctcaatcgg caaacgctgc actttagtgc gatagctgta gcggctgata
    18181 cgcgccgtac ctgggtcgat aacgtattta atacccggta cggtcagcga cgtttccgcg
    18241 acgttggtcg ccagcacaat gcgccgtcca ctgtgcgact ggaacacgcg gttctgttcg
    18301 ctgttcgaaa gccgcgcata aagcggcaag atttcggtat ggcgtaagtt cagcttgttc
    18361 agcgcatcgg cggtatcgcg aatttcccgc tcaccactca taaagatcag aatgtcgcca
    18421 gggctttcct ggctcagttc gtctacggcg tcaaaaatcg cctgcagctg atcgcgctcg
    18481 gtgtcatcgg cttcttcaac aatcggtcga tagcgcactt ccaccggata ggtccgaccg
    18541 gagacttcga taatcggcgc attattaaag tggcgcgaaa agcgttccgg gtcgatagtc
    18601 gcggaagtga taatgatttt taggtcagga cgccgcggta gcaactcttt caaatagccg
    18661 agcagaaaat cgatattcag gctacgttcg tgcgcttcgt caataatgat agtgtcgtac
    18721 tgcatcagca ggcggtcttg ctggatctcc gccagcagga taccgtcggt catcagcttg
    18781 accatcgtgt tatcactaac gtgatcactg aaacgcactt tgtaaccgat gcaaccgccc
    18841 ggctccgttt tcagctcttc cgcaatacgg ttcgccaccg ttcgtgccgc caggcgacgc
    18901 ggttgggtat gaccgatcag ccctttaatc ccgcgcccca gttccataca gattttcggt
    18961 agctgagtcg ttttaccgga accggtttcc ccggcgacga tcaccacctg gtgatcacga
    19021 atcgcttcga gtatgtcctg ctttttctga ctaaccggca aattgtccgg ataagtgatt
    19081 tccggtcgtg ccgcttcacg cagcaggact ttccctgccg catggtcaat ctctttcgcc
    19141 atctcctgga aaatggcctg ttgtgcatca ggatttttaa ccttcttcac gccgtgcaga
    19201 cggcgagaaa aacgcagtct gtcgcgcagc atcagcgaat ccagccgctg ctgcaaggcc
    19261 gtaaaggtca atttttgttg ttctgtcata acgttagtgg gcagtagtct gcctgattaa
    19321 ttttctttta aatgatagat atagagtacc acatcgcggc tttctatgcg ttgttcaata
    19381 aattcgaaca taggcttcga tttattgcgc tatctctgtg gcaggattgt gaataaagtg
    19441 tcaacaagca acggggcatc gcccattcaa acatctataa ggaaacacca tgagcaaggt
    19501 attagttctt aaatccagca tcctggcagg gtactctcag tctaatcagt tgtccgatta
    19561 ttttgttgaa caatggcgcg aaaagcactc cgctgatgaa atcaccgttc gcgacctggc
    19621 tgcaaatccg attccggtgc tggatggcga actggttggt gcgctgcgtc cgagcgatgc
    19681 accgctgact ccgcgtcagc aggaagctct ggcactttcc gatgagctga ttgccgagtt
    19741 gaaagcccac gacgttatcg ttattgcggc accgatgtat aacttcaaca tctcgactca
    19801 gttgaaaaac tattttgacc tggttgcacg cgcaggcgtt actttccgct atacggagaa
    19861 cggtccggaa ggcctggtca ccggtaaaaa agccatcgtt atcaccagcc gcggtgggat
    19921 ccataaagat ggcccaacgg atctggtgac gccgtatctg tccacattcc tgggctttat
    19981 cggcattacc gatgtgaaat ttgtcttcgc cgaagggatc gcatacggtc ctgaaatggc
    20041 agcgaaagcg cagtctgacg cgaaagcagc catcgacagt attgttgctg aataactgga
    20101 cacgatcttg atgtggactg tagatattca gtccacatct caatccgctt acctgttttc
    20161 ccataatttc agcatcgctt tgtgctcttc cttaagcaca atccgcgagc gtctggctcg
    20221 aatataacta atcgcttcat caacggtttt acaatgtccg taacataaca gccatgccgc
    20281 caccaccagc gcactgcgcg ataatcccaa cgcgcaatgg accagaacgc tgccttgctc
    20341 ttcgcgtaat gtttccagca tcgccacggc ctgtcggagc tccccctctt ccggaaccac
    20401 cagatccagc atcggtacac aaaaatagag ttgatctttt gtggctcgtc cgcgagggaa
    20461 ttcaaaggtg acgtccagaa cagcattctg tgccggaata tgttgtggaa acgcccctaa
    20521 ataaacacca gcagtaattt tgctcaccga ctccaggcga agacaaaacc aacgcataga
    20581 cagccacatc ccgatgcgcc agggcaatgt cagccagtaa acggcgggcg ggagtttccc
    20641 ctaactatct ttccctgttg ttatcgcgcc aagcccaccg tacccacggc caatgatgaa
    20701 tagcgataat actggccaac ataaccagac tgaccaccat aactgaagca ttatcattag
    20761 ctccatcaac acaatgcacg agcacgcgcc tacgacatat ggcagtgcta ttttgattcg
    20821 acgttgatca ggtttctgat aattccaacg acggtcgacg ggcaccatcc agtcaattaa
    20881 catacctacc gccagccctg tgatgacatc aataaaatga tgctgccagg tcgtcagcgt
    20941 cgaaatggcg atgagtaaaa accatccgcc gcagacttta cgccacctca cagccagatg
    21001 ctgacgaaag tgacgccaaa gtagccagca gagaataata tgcagcgaag gagactggtt
    21061 ataaggcaga tcaaacaatt caagttgcga aaataaccat cctgtcaccc cactcacttc
    21121 aggacggata aaactaaact tcagcggata gagaaaaaaa ccgcagcagg ccattaccgt
    21181 tgccagaata agccggtgga ccagacggcg ctgctcaaat gtcgagctac aaacaaacag
    21241 cgaaaatcca tataaaagat ccagactcca gtaaggaaca atagtccagg gaagaaaagg
    21301 gatcgccgtt tcccaaccga ataactgact ggggatatca tggttgttaa gatcctgagt
    21361 cgcggtgaac tgattaagaa atccatatgt gaagaagaaa aacggggcca gcaacaataa
    21421 ccagccagcg ccttgtagca atacgttccg gcgttcagtt accacgacgc accgccatcg
    21481 aaaccgtaaa aatgccccac tcatcaatcc gttgtgtgca tttatcgaat ccggcatcat
    21541 gcacgagcga atccatctcc ccttgagaac gcacgcgcat tacccacggt ttaccatctt
    21601 tatgactggt taacaccccg gcaatcagct ccagttgtgg gtgccacggc tgcccggtgt
    21661 agatgagaat gccacccgga tcgatggcat tggcaagacc tgcgagtgag ttttttacct
    21721 gctcattttc gggaaaaagc tcatacagtc cagagacaat cgccagcgta ggccgcggag
    21781 ttaacgcgct gagctcctcc gggttaaacg catcgccctg ttcaaaacgc acccgcccag
    21841 acattccccg ttgggcaatc atctcttgcc cctgtgcaac atttaactca ctgtaatcac
    21901 gtaagaaaat atcgcttacg gcaggctcgt tcgccagcgc atccagtaca tagcgtccat
    21961 gccctgcggc aatgtcaacc acgcggacgg ctaaaccttt agcgtggaga tcggcaacgg
    22021 cttgtttaat cagcatttgt aaatgggttt tgcgctggcg aataccgcgc cagccaacac
    22081 tgttcaggta gattttgtcg actaaacgcc cgaatgcgtt actaccttgc ggttgatttt
    22141 gatagacata atccagcgaa ctgcctgaat caaagccggt gctcattccg agatgcaggc
    22201 ccgcagagtg cgccccgaac aatttcatcg ctttacgcat aaagcgatac gccaaatcaa
    22261 ccggcgataa tggcacgggt ccaccagaaa gtagccgcca tcgatccgct gatggtcctg
    22321 tgcagtcttc atgctgataa tctaattttt gcgatttgtt agcatataag tgactaataa
    22381 agctttgcat tttttcaaat accagcgccc tgttctcttc acccaacgtg tcgtgataaa
    22441 agcctggcag cagatgcagc tctttcagag ggctacgtaa acgctgataa aaatcaatct
    22501 gcggttggcg gtgcaccaca tagtcatcgc ctgatatcag aagttgcgtg gggagcgtaa
    22561 tcgccgccgc atcgctaata atacgttcag acgttttata gagatctagc aagatattga
    22621 cggcaatcgc ccttgtgatc agcggatcat tattgaaact agccacccgt tgccgatcgt
    22681 gggtcaaata gcgtcctttc acataggaat taataaaaaa cagaccacgc agacgatgcc
    22741 ataacgccag cgcaggacgt gccagcggca catacaattt aaccttaaag gccggagaag
    22801 ccagcaccag cccgcgaata gcaggcgcat aatcatgaac ccatgtagca accatcactg
    22861 cgccgacgct ttgcgcgatc acaaccacct cttccagtcc gacctggctg tcgctggcag
    22921 caaaacggac aaattcatcg acatcttgca ctgaactcgc aagagacgga ctataaccac
    22981 gcggccccga agtttgtcca tgccctcggg catcccatgc ataaaaagca gtatctggca
    23041 tcgccagttc atcaacgaga tgttgtagac gaccagaatg ttcatgaccg cgatgaaata
    23101 agacgatgac ctttttcgcc cctggctgta aagtgggcca gtgccgataa aacaacgctg
    23161 tattatcact ggtggtaaaa aaatgttccc cagggatgcg tgaattttcc attctctcta
    23221 ttttccttca gtaacagcaa tagcgaataa aataaaaaaa taccggagcg gtgaaaatta
    23281 atgagtcaat cctgtcaagg agtccgccat gtcctggtaa tagttttcca ctatctttaa
    23341 caccaacatc tcgtttgatg gctgacatca ctacatcgcc gcaaaaacca ctaataccaa
    23401 ttaacaaacc cgctaataat gcctgtaatg tatctagcgg tgtcagtaac ggtccaataa
    23461 tcagtgacac aatcatggta gtgattacgc cccccaccag accttccagc gtttttcccg
    23521 ggctgacttt ggggaccact tttcttctgc cgcaggattt cccccataaa tactgtgcaa
    23581 tatcgtttga ctcggttaat gccaaaagaa ataacaccag taacgcctcg ccctggatat
    23641 ttgtggttgg caacattaat aaccaggcgg catgactcaa ggcgaaaacg gtcattatcc
    23701 agccacaaaa aatggcgctc acggtatgca gaaaaccgga gggttcaccc acaaaaactc
    23761 gccaggtggc taatatcaga aacccaaaca aaggtataaa taacagaaac agctcaaagc
    23821 aatttaaacc gataagcaaa tagttaagag gaataaccca atataaccaa cgcggaaaat
    23881 gtacggatat aagcgtacaa tattctttca atgccagaaa acttatcagc gcaaaaaacg
    23941 tcaacgtcac ccagcgtgga cctgaaatca ccagtgaaaa acaaatgacg atgccccacc
    24001 aggtacgaat acgtaatgta acttcatccc cttttctctc cggtaaccgc cataccagaa
    24061 aacggtttat cagcgttgct aaaattaaca acgcgaaaag tgttgccaga gatttttcca
    24121 gcattatttt tcagcgttca tcaggagcac actccgacaa cgattaatcg cagtccagag
    24181 aagcagaatt gaagcaatgc tccataaaag attattccat tgcatccact gtggataaat
    24241 ggcaacggcc agcacccaca acccaaatat taacgcgcga tcgcttttgc caaatggacc
    24301 ggcataactg cgaataccgt taatcgtctg cgcgagtaaa ccgcaaaact cagtcaatat
    24361 ggtgcaaaat aacatgagta ttacgagtga tgcgttactt tccggtaaaa ataaaaacgg
    24421 taaataaagc gcaatatcgg aaataacatc gccagtttca ttcaaaatcg ccccaagacg
    24481 tgtttgctga ttgcactcac gcgccatcat gccatccagc gcattgagtg ccatacggac
    24541 aaaaagcacg atgggcaata gcagaaacat gccatgatat ggcaccaacg ccaacaacaa
    24601 accggtgaga agagaaagtg ccaacgcggc aagggtgatc tgatttgcag acacttgccg
    24661 cttatgaagc caaaacatcg tcggccttaa cagtgactga aagagcggtt ttatctggta
    24721 gagcgtcatt atcaatcctt gataattctg tttgcgctat ggtgcatcaa ttgccctggc
    24781 agagatatcg caacacgccg cttcattaaa aattcagaat atatcaaggt attggggctc
    24841 actgtcagga atgattatcc ccaattaatc aaatagttaa aaatattacg ctgttattca
    24901 atgcaactca tcaagttttc tcatcgcttg ttcccgtatt gcgcgcgtct taccgttaag
    24961 cgccagctgg cggactaaat ccagttcagg gttaacaagc tctaacgcca gcaaaggatc
    25021 acgtttcagc ctcgccttta atacatcaac tggaaaacgg ggattattca tcgccacaat
    25081 ccaccactcg ggatcaattt ctgccgccag tactgcaggc ggtgtgtggc ggttgttaag
    25141 tgcgccgaac ttgaaaaatg tgagattgct tttcgccagt gtactccacc atatacgatc
    25201 cggttgtttt gccagtacat ccagttgtgc taacgcatcg ggttctagtg cataataaac
    25261 acgtaacgct tcgttaacct gcgcacggga actgttttcc tgcctcagaa aaaacttcgc
    25321 ccaaatatca tttcgtggcg tgttttgctg taattccttt aagccttggg agagttcgca
    25381 ttatggcaag atcaaatgtc gcaatgtctc gtccttcaaa tgcagcaacg ttgccttcat
    25441 ccagcatttg caattcctgc aataattcag gagaagactt agggcgaaac agcgcttctt
    25501 ttttcatctg attacagaca ttccctgcta tgcgaatatt gcttcgctta aaaatttctt
    25561 tcagagcatc ttcatcattt attaggcccc aataatatgc atcaggattt attatcatca
    25621 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
    25681 ttgtcgaaga tgcaacaaca ggtgcaatta tgtcattgat aaatatcccg cgcgcattaa
    25741 tacttccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
    25801 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgttacgt
    25861 tacgttacgt tacgttacgt tacgttacgt tacgttacgt taccgagaat aacccatgga
    25921 ttagaaagag agaaagtgga aatattacct ttgactgtca aattgccaag aacaatcaat
    25981 ttttgattac tctctatatt accttctacc attaaatcgc cacgatgcac tttccagtcc
    26041 gatgcagata aaccgacggg gacaagattc acgctactgt tgatagcttg ttcaacttgt
    26101 tgtttatctt cagcggtagc cgcagaaaaa aatggttcaa tatcaactgc atttacaaca
    26161 catcgaagtt ccgcctgtac tggaagactc agcataaaca ggggaagtaa acctatccag
    26221 ctgcaaatcc tgggcttaaa catatgcact ccatgccatt aaaaaagaaa caaaagggaa
    26281 atgcatcttt cccttttgta tattcgttat tctcgcgaaa taccgtccaa cgtagacaac
    26341 acttcctcag aaagatgcaa tttttcagca gccatattct cccgtaaatg cgcaacagaa
    26401 gacgtccctg ggatcagcaa aatattcggt gaacgctgta acagccacgc cagcgccacc
    26461 tgcattggtg ttgcgcccag gctcgctgct acatccgaaa gtgtggagga ttgcagcggc
    26521 gtaaagcccc caagcggaaa gaacggcacg taggcaatgc cgtcacgagc caaagcatca
    26581 atcattgcgt catcagcacg atgagcaatg ttgtattcgt tctgcacaca gacgatttcg
    26641 gtaatcttac gcgcctctgc aacctgcgtc ggtgtaacgt tgctcagacc aatatgtttt
    26701 accaggcctt gttgttgcat ctctgccagc acggtcaggc tggcttcaat cgatccttcc
    26761 gctgggccat gaccatcccc catcataacg cgcaggttaa ccacatccag tgcgcccagc
    26821 ccgagattac gtagattatc gtgcaccgct ttttgcagct ctgccggaga aaatgcgggc
    26881 aaccaggatg cgtcctctcc acgccgcgca ccaattttag tgacaattgt caggtcgtca
    26941 gagtaaggat aaagcgcttc gcggataatc tgattggtga cgtgcggacc ataaaagtcg
    27001 ctggtatcaa tatgattgac gcccaatgcc agcgcctcac gcagcacggt tatagcgacg
    27061 tggcgatctc ttggggggcc aaaaacccca ggacctgcca gttgcatcgc gccataacca
    27121 agacggttaa cggattttgt accgagagta aatgtattgc tgctcatcgc accctgccct
    27181 gtaataaaac ggattaagaa aaatagtata gaaacgaaaa gagataaata atgcgaaggc
    27241 gtaaacaaga ttactggatg agttgctcta ttgccctgaa agacaccaaa acaaaacgcg
    27301 tcaagtaccg catccggcaa taaaaaaacc gcaacctgta tcagcgttgc ggtttaggta
    27361 tcacatgcta aaaattagaa cgtccagcat caggacgtta ctgtttcggt cgcagattct
    27421 catcaaatac caggcgctgc ctgccaggtt caacttcccg caatggctca acttgatgca
    27481 acgtctgctt actgcgtgtc accagcacct gatactcatg cgtgccctgc tttttccatg
    27541 ccagttcctg ctcactaagt tcacgaatcg cttttgccgg gctgccgaca atcaggtaat
    27601 tagcaggcat ttctgctttg gctttcacaa atgcggaagc gccaacaatg ctgttctcgc
    27661 caatcaccgc accgtccatc actaccgcgt tcatacccac taatgcattg cggtggataa
    27721 tgcagccgtg aaggatagcg ctatgaccaa tatgcccatc ttcttccacg acagtatcct
    27781 gatcgggaaa accgtgcata acgcaattat cctgaatgtt cgcgccatct ttcaccacga
    27841 tacgaccgaa atcgccacgc aggctggtat ttggcccaac gtacacgccc ttgccgagaa
    27901 taacatcgcc gatcaatacc gctgtcggat ggacaaaact ctcttctggc acaaactgag
    27961 tcagaccgtc tatctgataa attggcataa cgcctcctgt tcaatattca agccgccaaa
    28021 acgttgaaaa tacaggcttc ccggcgcagg caattcaccg accgaggttt cacctttttc
    28081 actaacgaac gctaaagcag caggcgctac gcgctgataa agtttgccga taaaggtttg
    28141 gtagtcgcgc aatatatccg taaccgtcgt ctcgattttt gtgcagatgc gattcgccat
    28201 gccgcagatg atgaaaaact ggcaggcatt ggctttcatt ggggattttc cgaccagagt
    28261 catttttcaa cggtatttaa gcaacgcttt gggatgacgc caggcgagta tcgacgtaaa
    28321 ttccgctaac taattcgcgt tgcatgtaca gcaacgcgaa ttagttaaaa cagatatttg
    28381 actatgcatt tttcagggcg aaaggcaatt tgacaggaat ttttgaatat tcacgttgtt
    28441 tccggaaaat agattaatcg tcttctcctg tagcgttatc ccgatattgc ggttttgcta
    28501 ttaaaatttt cgcacccatt taccgtttat cccttgtaca tattccccgg gtttggcccg
    28561 ttctacaagc ttttgcccgg cgagtttcgc gatatcatcc accgacacat tattctgctt
    28621 tgccagttgt tgatagctcg ctttacgttc ggcattgatg tcggttacta atttctcagt
    28681 ttcagcatct gttttcagcg caaccagata accataaaat gtttcgccta cccgcccctg
    28741 agttctggct tcatccagag tcaatgccat tacattgctg cttaccaagc caacaagaaa
    28801 tgcacaaaga agtaatgttc ttttcatcat cacctcaaaa aagatcgcta cgggtctcaa
    28861 gcagctcttc gacatctttg tctgccttga tgatgatctc atgctcaatt ttaacgttca
    28921 tattgatagt gatcggttcc ttaggtgcag cgacttcaat gcgaggcgta cagccaacca
    28981 gcataaaaga tgacgtcaac gcagccagta aaattttcat tgtttatcct cacactcttt
    29041 tccttgcgga cagtcatttc ccggtagatg cgcgttctgc tccagccatg cctggagatt
    29101 atcgccaaag cgtaaactgc gccacagcgt aaaaatattc tcttcatgat ggtaattaag
    29161 atttaccgta ccgcttttac catcaacgcg actggtacct gtaatattgg cctgcatggt
    29221 taataaaccg agattatcta aattaatttt tgtcgatgaa cggctaattt ccatatagcg
    29281 caaccagtta attgctgaac ccgcggtcat attgtctttc accaccgcat ctgcggtgtc
    29341 tttatccagc cgcaaggtca ttggcccgct atttgccagc cagccgtctt tcactatcca
    29401 tttttcgttg tttagccata acggcaatgc accactaaat gccccggaca tggcgaattg
    29461 tttcggattg acggcgctaa ccagttcgct ggatgagaga ttattcagcc gcaacagcgc
    29521 cgagtcatgt tgcggcatgc gtaattgttt catcagcacg ttaccgccta acacatcgac
    29581 gctaacatca gtcaacagca atggttcgtc ttcactccac ggataacgcc cttgcaaatc
    29641 agccgtaata tttttcgctg tcaccagatt aatcacttcg gcaattcgca acgtaacggg
    29701 gccgcgagtc cccagatgcc aggctccatc ggcaaaacgg aaaggcagga caaaatcgac
    29761 accgttaacc tgattatctg gcatccaggc actaccgcct ttcaacacac cgtgccctcc
    29821 cgcgcggaat ccttgttcag gcgcagctga aaatgcaacc tgagcataca gttcgccatc
    29881 gcgtaagttc atcttccagt cgggtggcac cagcggctgg aatacagtca gtgattgttt
    29941 aggccaccag gcgttaccgc gcagacgaat accgtcccag cgaccattta cccgaaccgg
    30001 gccaatctca ccagcatgca aatcgccttt aaagaggaaa tacgtcggat cgcgcccatc
    30061 gacactaaat tttaaggttg atggcggtaa cacactgccg ccagtgaaca gcgtttgccc
    30121 ggcgtccagt gacagcgcgc cgctaaagga gggatgctgt gcgtcacgta cccagacgac
    30181 gggcttgtcg agaattaatc gcggcttttc taccgtcatc gtaccgtatt gtaactggtc
    30241 gaaaccggtg gagagatcgg tcagcgtaat cgtgctgtca tgccactcac cctttcctgc
    30301 gacatcccag gtggcattca tcggtgtaaa actgcctttt ccccagtagc gccactgcca
    30361 gcggccagcg tcagggagaa aatcattcgc cagtccatcc atatgcagca cgaaatcgcc
    30421 cagttcattt tcatgcgcct gcaaaatggc ctgcaaatgt ccgtcaacac cacgttgggt
    30481 gacttttaca cccgctaaag gccagcggat ttcatcgatg tccagcgaat cgatgactct
    30541 tcccttcgaa cgtaataacg cgcctggctc aaaggccagc gttgggtcag tcagacttcc
    30601 gcttagctgc gcaggtaaac gggcatataa aatgaggtcc gcctgtttcg cttcaccggt
    30661 cagctgcaaa ggcagctggc tgttatccat acttaatttt cctgggccaa aattaagcac
    30721 cgcgttgccc ttacccgctt gcccctgggt cagcacactc agtcgtccgc tgaccagagc
    30781 gttctcgagc cctgcctgcc agttgtcgac tttgacaccc agtcgtccac tcaaaggaaa
    30841 acctgcatac ggccagctcc agcgaccatc gcttacggtc aattgttggc gagtaatttg
    30901 ccacggcaga tcgagcaacg gatcgccgtt atcccgtgcc accacaatca attgcccgct
    30961 attttcctgc cagtccagct cggcatccac cagtgaaggc tcctgcggca agtttaacgt
    31021 cgcagtagca tgaccactta caggaagtcc atccggcact agcggcatag taaattcccc
    31081 caccagttta accggcggct gattttcaaa cgcgacgaca tccagttcgc tgactgtaag
    31141 ttgttgccct ttcagctggc cttgaaattt aactttttcg ccctgataac gcagttgctg
    31201 gatatcggag gttaatgcga gagagagttt tccctgccat tcctgccagg gagaaaaaat
    31261 cagtttatcg atattgatcc aggtgttagg caacatggcc tgccactgcg cgagggtttt
    31321 tggagcggca ggcgattgct ccgtctgcgg caatttcgcc agacaagcag aatcaagttc
    31381 taccgtgccg acgttcaata accagcggct gggatgtgaa aggctggcgt tggtgatatg
    31441 cgcaagctga caatctccca ccagataacg gagatcggga atgattaaac ctttacgcgt
    31501 aatgcgtggg ctttcatcta atgcaatacg tgtcccgagc ggtagccaga tgcccgccag
    31561 cgtgggaacc cacagcccga gcgtcatcag cagtgtcaac ggcacaagaa taatcagtaa
    31621 taatagcgcg agaacggctt tatatttacc cagcatgggt agttaatatc ctgatttagc
    31681 gaaaaattaa gcattcaata cgggtattgt ggcatgttta accgttcagt tgaaggttgc
    31741 gcctacacta agcatagttg ttgatgaatt tttcaatatc gccatagctt tcaattatat
    31801 ttgaaatttt gtaaaatatt tttagtagct taaatgtgat tcaacatcac tggagaaagt
    31861 cttatgaaac tcgccgttta tagcacaaaa cagtacgaca agaagtacct gcaacaggtg
    31921 aacgagtcct ttggctttga gctggaattt tttgactttc tgctgacgga aaaaaccgct
    31981 aaaaccgcca atggctgcga agcggtatgt attttcgtaa acgatgacgg cagccgcccg
    32041 gtgctggaag agctgaaaaa gcacggcgtt aaatatatcg ccctgcgctg tgccggtttc
    32101 aataacgtcg accttgacgc ggcaaaagaa ctggggctga aagtagtccg tgttccagcc
    32161 tatgatccag aagccgttgc tgaacacgcc atcggtatga tgatgacgct gaaccgccgt
    32221 attcaccgcg cgtatcagcg tacccgtgac gccaacttct ctctggaagg tctgaccggc
    32281 tttactatgt atggcaaaac ggcaggcgtt atcggtaccg gcaaaatcgg tgtggcgatg
    32341 ctgcgcattc tgaaaggttt tggtatgcgt ctgctggcgt tcgatccgta tccaagtgcg
    32401 gcggcgctgg aactcggtgt ggagtatgtc gatctgccaa ccctgttctc tgaatcagac
    32461 gttatctctc tgcactgccc gctgacaccg gaaaactatc atctgttgaa cgaagctgcc
    32521 ttcgaacaga tgaaaaatgg cgtgatgatc gtcaatacca gtcgcggtgc attgattgat
    32581 tctcaggcag caattgaagc gctgaaaaat cagaaaattg gttcgttggg tatggacgtg
    32641 tatgagaacg aacgcgatct gttctttgaa gataaatcca acgacgtaat tcaggatgac
    32701 gtattccgtc gcctgtctgc ctgccacaac gtgctgttta ccgggcatca ggcattcctg
    32761 acagcagaag cgctgaccag tatttctcag actacgctgc aaaacttaag caatctggaa
    32821 aaaggcgaaa cctgcccgaa cgaactggtt taatcttgcc gctcccctgc attccagggg
    32881 agctgattca gataatcccc aatgaccttt catcctctat tcttaaaata gtcctgagtc
    32941 agaaactgta attgagaacc acaatgaaga aagtagccgc gctcgttgcg ctaagcctgc
    33001 tgatggcggg atgtgtaagt aatgacaaaa ttgctgtaac cccagaacag ctacagcatc
    33061 atcgctttgt gctggaaagc gtaaacggta agcccgtgac cagcgataaa aatccgccag
    33121 aaatcagctt tggtgaaaag atgatgattt ccggcagcat gtgtaaccgc tttagcggtg
    33181 aaggcaaact gtctaatggt gaactgaccg ccaaagggct ggcaatgacc cgtatgatgt
    33241 gcgctaaccc gcagcttaat gaactcgata acaccattag cgaaatgctg aaagaaggtg
    33301 cacaagtgga tctgaccgcg aaccagttaa cgctggcgac cgcgaaacag acattaactt
    33361 ataagctggc ggatttaatg aattaatagc tgccacagct cccggcggca agtgactgtt
    33421 cgctacagcg tttgccgttg ggtaatgcac acatcccaat cgtcgtacca tccagttgac
    33481 gggcaacaga aagcgaaccg ccgatcattg cacaatttgc ttctccacta ctggacatcg
    33541 acgcttttaa acctggcgct acgtgcgccg cagtcgcctg ctgaacaggt tcactactac
    33601 acgccgacaa caataaagcg gcacacccta cccaaaacgc tgctcgcatc ttttcttcct
    33661 ctgatcttca agccaaacga caccgccata aataataggc agcacagagg gcggcgtcga
    33721 gagctgtcct gagcgttgcc ccgcattttt acttttttat ggctattttt ttgccctctg
    33781 tttgatcaaa acattcatta cgctgatgtg ggggacacaa aagcgaaaat gcagaagaaa
    33841 gccatttgct aaaattgaag gattactact gggcgcgcag caatttcgtg cgcccctcat
    33901 ttgcgcaatg taagggtgtc atatgattac tattgacggt aatggcgcgg ttgcttcggt
    33961 ggcgtttcgc accagtgaag ttatcgccat ctaccctatt acccccagtt ccacgatggc
    34021 agaacaggct gatgcctggg ccggaaacgg cttgaagaac gtttggggag acacaccacg
    34081 cgtggttgaa atgcagtcgg aagcgggtgc tatcgctacc gtgcatggcg ctttgcagac
    34141 gggtgccctc tcaacatcgt ttacgtcatc gcagggtttg ctgctgatga tcccgacgct
    34201 gtacaaactg gcaggcgaac tgacgccatt tgtcctgcat gtagcggcac gtaccgttgc
    34261 cacacatgca ctctctattt ttggcgatca ttccgacgtt atggcggtgc gccagacggg
    34321 ttgcgcgatg ttgtgtgcgg ctaacgtcca ggaagcgcaa gactttgctc tcatttcgca
    34381 catcgcaacg ctgaagagcc gcgtgccatt tattcatttc tttgatggtt tccgcacgtc
    34441 ccacgaaatc aataaaattg tcccgctggc cgatgacact attcttgatc tcatgccgca
    34501 ggccgaaatt gatgctcatc gcgcccgggc gctcaacccg gaacatccgg tgatccgcgg
    34561 tacgtccgcc aatcctgaca cttatttcca gtctcgcgaa gccaccaacc catggtacaa
    34621 cgcagtctat gaccatgttg aacaggcgat gaatgatttc gctgccgcga caggtcgcca
    34681 gtatcagccg tttgagtatt acgggcatcc acaagcggaa cgggtgatta tcctgatggg
    34741 ctctgccatt ggcacctgtg aagaagtggt tgatgaattg ctgacccgtg gcgaaaaagt
    34801 cggcgtgctg aaagttcgcc tgtaccgccc cttctccgcc aaacacttac tgcaagcgtt
    34861 gccggactct gttcggacag tggcggtgct ggatcgcacc aaagagccag gtgcgcaagc
    34921 ggaacccctg tacctggatg tgatgaccgc actggcagaa gcctttaata atggcgagcg
    34981 tgaaaccctg ccccgtgtca ttggtgggcg ctatggtctt tcatccaaag aatttggccc
    35041 ggactgtgta ctggcggtat ttgccgagct caacgcggct aaaccgaaag cgcgctttac
    35101 ggtaggtatt tacgatgatg tgaccaatct gtcactgccg ttgccggaaa acaccctgcc
    35161 aaactcggcg aaacttgaag ccttgtttta tggccttggt agtgatggca gcgtttccgc
    35221 gaccaaaaac aatatcaaga ttatcggtaa ttccacgccg tggtacgcac agggctattt
    35281 tgtttacgac tccaaaaagg cgggcggcct gacggtttct caccttcgcg tgagcgagca
    35341 gccgattcgt tccgcttatc tgatttccca ggctgatttt gttggctgcc accagttgca
    35401 gtttatcgat aaatatcaga tggctgaacg tttaaaacct ggcggcattt tcctgctcaa
    35461 cacgccgtac agcgtagatg aagtatggtc gcgcttgccg caggaagttc aggccgtgtt
    35521 aaaccagaaa aaagcgcgct tctatgtgat taacgcggcg aaaatcgccc gcgaatgtgg
    35581 tctggcggcc cgtattaata ccgtcatgca gatggctttt ttccatctga cgcaaattct
    35641 gcctggcgat agcgccctcg cagaattgca gggtgcgatt gccaaaagtt acagtagcaa
    35701 aggtcaggat ctggtggagc gcaactggca ggctctggcg ctggcgcgtg aatccgtaga
    35761 agaagttccg ttgcagccgg ttaatccgca cagcgccaat cgaccgcctg tggtttccga
    35821 tgccgctcct gatttcgtga aaaccgttac cgcggcgatg ctcgccgggc ttggtgatgc
    35881 cctccccgtt tcggcgctgc cgccagacgg cacctggccg atgggcacca cgcgctggga
    35941 aaaacgcaat atcgccgaag agatccccat ctggaaagag gaactctgta cccaatgtaa
    36001 ccactgcgtc gctgcttgcc cacattcggc tattcgcgca aaagtggtac cgcctgaagc
    36061 gatggaaaac gcccctgcca gcctgcattc gctggatgtg aaatcgcgtg atatgcgcgg
    36121 gcagaaatat gtcttgcagg tggcaccgga agattgcacc ggctgtaacc tgtgcgtcga
    36181 agtttgcccg gcgaaagacc gtcagaatcc agagattaaa gccatcaata tgatgtcgcg
    36241 cctggaacat gtcgaagaag agaaaatcaa ttacgatttc ttcctcaacc tcccagaaat
    36301 cgaccgtagc aaactggaac gtattgatat tcgtacatca cagctgatta caccgctgtt
    36361 tgaatattca ggtgcttgct ccggttgtgg cgagacgccg tatattaaat tgctgactca
    36421 gctctatggc gaccgaatgt tgatcgcgaa cgccaccggc tgttcttcaa tttatggcgg
    36481 taacctgccc tctacaccgt atactaccga tgccaacggt cgtggaccgg catgggcgaa
    36541 ctcgctgttt gaagataacg ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca
    36601 ccgtgtccgc gtgttgcgtc tgctggatca atttgccgat aaaatcccgg cggaattact
    36661 aactgcgttg aaatcagacg ccacgccaga ggttcgtcgt gaacaggttg cagctttacg
    36721 ccagcaactc aaagatgttg ccgaagcaca tgaactgcta cgtgacgcag atgcactggt
    36781 agaaaaatca atctggctga ttggtggtga tggctgggct tacgatatcg gctttggcgg
    36841 tctggatcat gtattgagtt tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg
    36901 ctattccaac accggtggtc aggcgtcgaa agcgacaccg ctgggtgcag taactaagtt
    36961 cggcgagcac ggcaaacgta aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg
    37021 tcatgtttat gtggcgcaga tttctctcgg cgcgcagctc aaccagacgg tgaaagcgat
    37081 tcaggaagcg gaagcgtatc cggggccatc gctgatcatc gcttatagcc cctgtgaaga
    37141 gcatggttac gatctggcac tcagtcacga ccagatgcgc caactcactg ccaccggctt
    37201 ctggccgcta tatcgcttcg atccacgtcg agccgatgaa ggcaaactgc cgctggcatt
    37261 ggattcacgc ccgccgtcag aagcactgga agaaacgtta cttcacgagc agcgtttccg
    37321 tcgtctgaat tcgcagcagc cagaagtggc agaacagtta tggaaagatg ctgcagctga
    37381 tttgcaaaaa cgctatgact tcctggcaca aatggccgga aaagcggaaa aaagcaacac
    37441 cgattaaatg cactggataa ggattatcca attctaaaaa aaagcccgga cgactgttcg
    37501 ggcttgtctt tttatatgtt ggaaaatcag tagcaatgca atggcccaac agaaaataaa
    37561 atctgtgaca ataaaggcat ataacccgcg cagaataacg tatagcaaat taaaagtata
    37621 atttttattt tgtatgtatt atcttcaata cattcaccag acttattatc atttcagaag
    37681 aattatttga ttaaggtttt acttaaggcg taacaaatga tatttatcgg ctaactgaac
    37741 ttctccttta ggatgctttc actccccaaa atggggataa aaggcaaata aaataactaa
    37801 aggatttatt caatgaaaag caaagtactg gcacttttaa ttcctgccct gctcgccgca
    37861 ggtgctgcac atgcagccga agtttataat aaagacggca acaaattaga tctgtatggc
    37921 aaagttgatg gcctgcatta tttttctgat aattcagcga aagatggcga ccagagctat
    37981 gcgcgtctgg gttttaaagg cgaaacccaa attaacgatc aactcactgg ctacggtcaa
    38041 tgggaataca atattcaggc aaacaacact gaatcttcaa caaaccagtc atggacccgt
    38101 ctggcatttg ccggactgaa atttgcagat tacggttctt tcgattacgg acgtaattat
    38161 ggcgtaatgt acgacatcga aggctggacc gatatgctgc ctgaatttgg cggtgactct
    38221 tataccaatg cagacaactt tatgactggt cgtgccaacg gcgtcgcgac ttatcgtaat
    38281 actgatttct tcggtctggt aaatggtctg aacttcgcgg tgcagtatca aggtaacaac
    38341 gaaggagcca gtaatggtca ggaaggcacc aacaacggac gtgatgttcg ccatgaaaac
    38401 ggtgacggct ggggtctttc cacaacatat gatttaggca tgggctttag cgctggtgcg
    38461 gcatacacct cttctgaccg caccaatgac caggttaacc atactgcggc gggtggtgat
    38521 aaagcagacg cgtggactgc tgggctaaaa tacgatgcta acaatattta cctggcaacc
    38581 atgtattcag aaacgcgtaa catgaccccg tttggcgaca gcgattatgc agttgcgaac
    38641 aaaacccaga actttgaagt tactgcacag taccagtttg attttggtct gcgtccggca
    38701 gtctctttcc tgatgtctaa aggccgtgac ctgcacgctg ccggtggtgc agacaacccg
    38761 gcaggtgttg atgataaaga tctggttaaa tacgccgatg ttggcgcgac ttactatttc
    38821 aacaaaaata tgtccaccta cgttgactat aaaataaacc tgttggatga agatgacagc
    38881 ttctacgctg ccaacggcat ctctaccgat gatattgtcg ctttaggtct ggtttatcag
    38941 ttctaaatcc tcctgcccgc tgttatggcg ggctttttct gcttattctt cctcctttga
    39001 tctaaattaa aaatgtgaac tccgtcatta cacaaaaagt gtcatctggc gttacacttt
    39061 atgcggatac taaaacagga ggttttatga acagaacgat tcttgtccct atcgatattt
    39121 ccgattcaga attaactcaa cgcgtgatta gccacgttga ggcagaggca aagattgatg
    39181 atgcagaggt tcatttcctg acggtaatac cttcactgcc ctactatgcc tctctgggtt
    39241 tagcgtattc cgcagaatta ccggcaatgg atgacctgaa agcggaagcc aaatcgcaac
    39301 tggaagagat cattaaaaaa tttaaactgc caaccgacag agtgcatgtc catgttgagg
    39361 aaggctcgcc caaagaccgc attctggaat tggcgaagaa gatccccgct catatgatca
    39421 tcattgcttc ccatcgaccg gatatcacca cttatctgct cggttctaac gccgcagcgg
    39481 tagtgcgtca cgcagagtgc tccgtgctgg ttgtgcgctg acattaacgc ccgcacattg
    39541 ctgcgggctt tttgattcat ttcgcaaatg tgctgacatt ttcccctcta atccgtacca
    39601 tacacgccac agtttttata tcagatttct tatgctgggc gttccggcat gacgcatact
    39661 cttctgatgc catataacga attgagtcgc ttttaaatgt cgcaaaatca agaaattagt
    39721 aagaaagaac aatacaacct gaacaaatta caaaaacgtc tgcgtcgtaa cgtgggcgaa
    39781 gccattgctg acttcaatat gattgaagaa ggcgatcgca tcatggtttg cctctccggg
    39841 ggtaaagaca gctataccat gctggaaatt ctgcgcaatt tgcagcaaag cgcgccaatc
    39901 aatttttcgc tggtggctgt taacctcgat caaaagcaac cgggcttccc ggaacacgtt
    39961 ctgcccgagt atcttgaaac gctgggcgtt gagtacaaga ttgtggaaga gaacacttac
    40021 ggtatcgtga aagagaagat tccggagggc aaaaccactt gctcactgtg ttctcgtctt
    40081 cgtcgcggta tcctttatcg caccgcaacg gaactggggg cgacgaagat cgcgctgggt
    40141 caccatcgtg acgatatcct gcaaacgttg ttcttaaata tgttctacgg cggcaagatg
    40201 aaaggtatgc ctccgaaact gatgagcgat gatggcaaac atattgtgat tcgtccgctg
    40261 gcctactgcc gcgagaaaga tattcagcgc tttgccgatg caaaagcgtt cccgattatt
    40321 ccgtgcaacc tgtgcggttc acagcctaac ctgcaacgtc aggtgattgc tgacatgttg
    40381 cgtgactggg ataaacgtta tccagggcgt atcgagacga tgttcagcgc gatgcagaat
    40441 gtggtgccgt cgcatctgtg cgataccaac ctgttcgatt tcaaaggtat tacccacggt
    40501 tctgaagtgg ttaacggggg tgatctggcg tttgatcgcg aagagatccc tctacaaccg
    40561 gcgggttggc agcctgaaga agatgaaaat cagttggatg agttacggct gaatgtggtt
    40621 gaagtgaaat aacccggata gcgcccgatg cgcaagctta ccgggctact cttatggagg
    40681 ccggataaga cgcggccagc gtcgcatccg gcaatcccga agaagatgtt tactcttgca
    40741 ccctgcgatt caacatttca ttattttaat aaccgcaccc ggcacgtttt tcctttaatc
    40801 ttcccgccct gtaactgttt ccatgcttta tgagcaacag cctgacgcac cgcgacatag
    40861 acatgcgccg gatgcacggc gattttgcca atatctgcgc catcaagccc gatatctcct
    40921 gtcagcgcac ctaatacatc acccgggcgc attttggctt ttttcccgcc atcgatacat
    40981 aacgttgcca tttctgcttc cagcggcaca atggaactat taactggcgg cgtttgccag
    41041 ttaagtttta tctgcaacat gtcagaaatg atattggccc gctgtgcttc ttccggggca
    41101 cagaaactga tcgccagacc gctatttcct gcacgagctg tacgaccgat gcgatgtaca
    41161 tgaacttcag ggtcccacgc cagctcaaag ttcaccacca gctcaagcga ttttatatcc
    41221 agaccacgcg cagcaacatc agtcgcgacc agtacacggg cgctaccgtt agcaaaacgt
    41281 accagggtct gatcgcgatc gcgttgctcc agatcgccgt gtaacgacaa tgcactttgc
    41341 cctacttcat tcagcgcgtc gcagacagac tggcaatctt ttttggtatt gcaaaacacc
    41401 acgcaagagg atggctgatg caagcttaat aaccgttgca acagaggaat tttgcctttg
    41461 ctggatgtct cataaaattg ttgttcaata ggtggcagag catctgttga gtcaatttca
    41521 atcgctaaag gatcgcgttg cactcttccg ctgattgcgg cgatggcttc cggccaggtt
    41581 gccgaaaaca gaagcgtctg tcgagatgct ggcgcaaaac ggatgacatc atcaatggca
    41641 tcgctaaatc ccatatccag catgcggtcg gcctcatcca tcaccagcgt attcaacgca
    41701 tccagtgata ccgtgccttt ttgcagatga tccagcatac ggcccggcgt tgccacgata
    41761 atatgcggcg catgttgcaa cgaatcacgc tgcataccga acggttgacc accgcacaac
    41821 gtcaaaattt tggtatttgg cagaaaacgc gccagccgac gcaattcacc tgccacctga
    41881 tccgccagtt cacgcgtagg acacagcact aaagcctggg tttgaaatag cgacgcatca
    41941 atttgctgta acaagccgag gccaaaagcc gccgttttgc cgctgccggt tttcgcctgc
    42001 acgcgaacat cttttccggc aaggatcgtc ggaagcgcgg cggcctgcac cggcgtcatg
    42061 gttaaataac ccaactcatt aaggttcgtg agttgggcgg gaggcaaaac attcagggta
    42121 gaaaaagcgg acacaattta ttctcgtggt catcgacgca aagtcagcag gcgcgtatcc
    42181 tcgcagatct acgctcacga tgcgacaatt taatcggttc ttcgtcgggt ggtgggtcag
    42241 gcatgggttg cgggcgaggg atcggatcgg gcactggaac aggatcgcca ggaatcggtt
    42301 cagggacagg aatttgcaaa taaataagtg tcgtcatatt tccctccggt cattgggtgg
    42361 actcttaaag ggtagacgct gacaaataac aggcaaaaaa aagccgactc atcaaagtcg
    42421 gcgtcgtacg aatcaattgt gctatgcagt aattcaaaaa aggaagtaag acaatatgga
    42481 gcgcaacgcc catcgcttga cgttgcattc acctgcaaga gagatattgc cctgaatggg
    42541 tagagagttt attgacttcg ctcaaacttt gcggcgtttt tgtatacaga cagccgggaa
    42601 aattgctttt gttacaacca tttactacga tgcaaccata aagcaacacc accaataaga
    42661 acaactaaca gaatacaaaa aattgaaaat ccgaattgcc acccgccgcc tgggatccca
    42721 ccaaggttga cgccaaataa ccctgtcaga aaggtactgg gtaaaaagac cattgccatc
    42781 aacgacattg tataggtacg acgcgctaaa ttttcctgca tcacctgagc gatttcatca
    42841 gccatcacgc cagtccgtgc tatacaggcg tcgatttcgt caaggccgcg cccaaggcga
    42901 tcggcaatat cctgcatacg acggcgttgg tcatcgctca tccacggcaa acgttcactg
    42961 gcaagacgag cataaacatc acgttgcggt gccatatagc gacgcatcac aattaattgt
    43021 ttgcgcagca gagccaggaa tccacgcggt ggaatttgct gatcaaggag attatcttca
    43081 aggtcgataa ttttatcgtg cagctgctcg ataaattcgc tggaatgatc ggtcaacgca
    43141 tcacacacat ccaccagcca tcccccgcaa tcggtcggac cggtgccttc ttccagatcg
    43201 cttaccacat cgtccagcgc cagcactttg cgttgtcggg tcgaaacaat taaccgcccg
    43261 tccatatata cacgcatggc gaccagttga tcggggcgtt catcggtgct gccgtttata
    43321 cagcgcaatg taatcagcgt accttcaccg agacggctga ctcggggccg tgtgctctcg
    43381 cccgccagcg catcacgtac gttattggga agcagcggtg ttgtcgccag ccattgggcg
    43441 ctatcatggt gtacataatt aaggtggagc cagcaggggt gcgcttcatc aatcacatct
    43501 gtattttcca gcggtttaac gccgcctcta ccatccagca tccaggcaaa tactgcatcc
    43561 gggacattaa cgtccgctcc cttaatcgct tccacagtgc ctccatcatc aacgcattat
    43621 tttgtagtct agccttctgg ccctgttacg caacatctca tcaccccatt accctgaaat
    43681 gattaataaa attctgtcta aattgaata
//
LOCUS       DAZGHF010000023        42044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGHF010000023 DAZGHF010000000
VERSION     DAZGHF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.023"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            52..2229
                     /gene="sinH"
                     /locus_tag="ACHK3A_001404"
     CDS             52..2229
                     /gene="sinH"
                     /locus_tag="ACHK3A_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFO8739512.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYIINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDEWLLGGNIFYDYDFTRGHRRLGLGGEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKVVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGADYKAGTGDNSDVSINATLNYQFGVPLKDQLDSDKVKAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPRLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDSNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
     gene            2284..3261
                     /locus_tag="ACHK3A_001405"
     CDS             2284..3261
                     /locus_tag="ACHK3A_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFO8739513.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEESAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADHVTLTSDLKGARPTGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDDDRGRYIGIEITPTT
                     QTGTPNVGTALHLYDISTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLAIPATNDEARTNLNGPARDGKEALTIPTNGDGVQGYKLHIIYKHK"
     gene            3382..11433
                     /locus_tag="ACHK3A_001406"
     CDS             3382..11433
                     /locus_tag="ACHK3A_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFO8739514.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPAKTHEVAFTAKPSEFSADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPTVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLIAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKTTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQTSETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEDGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNYYWSSLAGMSGQHYAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGVNR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEASLTLTQPGGVGVKTHITAK
                     MRSDYTASDEKDVIFTVITSPDSDKARMWGHMLGIIEANNIFKRPRLADETDNELGAV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANVIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDHDVSTSSRAQAKVG
                     ETITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQPQGVGLATPLNIAPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVSSPDGTLTENNEVWSRVSQANTSSTSKG
                     GCGANMLPRRSQLNALYDANSGNAVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDMPFTLKRGDGYTRSNEKHIAGGGDALVAPVVINSGLADETSLNDTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTAALYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGTVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDSTNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQPTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMPVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGDVV
                     AMDDLIVQPLSGSAVTLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(11502..12878)
                     /gene="xseA"
                     /locus_tag="ACHK3A_001407"
     CDS             complement(11502..12878)
                     /gene="xseA"
                     /locus_tag="ACHK3A_001407"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO8739515.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDNAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQLKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANLRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="ACHK3A_001408"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="ACHK3A_001408"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO8739516.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="ACHK3A_001409"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="ACHK3A_001409"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO8739517.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16245..16784)
                     /gene="yfgI"
                     /locus_tag="ACHK3A_001410"
     CDS             complement(16245..16784)
                     /gene="yfgI"
                     /locus_tag="ACHK3A_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO8739518.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16800..17318)
                     /gene="yfgH"
                     /locus_tag="ACHK3A_001411"
     CDS             complement(16800..17318)
                     /gene="yfgH"
                     /locus_tag="ACHK3A_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO8739519.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17629..17820)
                     /gene="yfgG"
                     /locus_tag="ACHK3A_001412"
     CDS             complement(17629..17820)
                     /gene="yfgG"
                     /locus_tag="ACHK3A_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO8739520.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17838..17990
                     /locus_tag="ACHK3A_001413"
     CDS             17838..17990
                     /locus_tag="ACHK3A_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739521.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     YVIMMS"
     gene            18172..20415
                     /gene="pdeF"
                     /locus_tag="ACHK3A_001414"
     CDS             18172..20415
                     /gene="pdeF"
                     /locus_tag="ACHK3A_001414"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO8739522.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEISQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(20454..21995)
                     /gene="ppx"
                     /locus_tag="ACHK3A_001415"
     CDS             complement(20454..21995)
                     /gene="ppx"
                     /locus_tag="ACHK3A_001415"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO8739523.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(22000..24066)
                     /gene="ppk1"
                     /locus_tag="ACHK3A_001416"
     CDS             complement(22000..24066)
                     /gene="ppk1"
                     /locus_tag="ACHK3A_001416"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO8739524.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(24237..24875)
                     /gene="purN"
                     /locus_tag="ACHK3A_001417"
     CDS             complement(24237..24875)
                     /gene="purN"
                     /locus_tag="ACHK3A_001417"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO8739525.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(24875..25912)
                     /gene="purM"
                     /locus_tag="ACHK3A_001418"
     CDS             complement(24875..25912)
                     /gene="purM"
                     /locus_tag="ACHK3A_001418"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO8739526.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            26237..26863
                     /gene="upp"
                     /locus_tag="ACHK3A_001419"
     CDS             26237..26863
                     /gene="upp"
                     /locus_tag="ACHK3A_001419"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO8739527.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            26949..28238
                     /gene="uraA"
                     /locus_tag="ACHK3A_001420"
     CDS             26949..28238
                     /gene="uraA"
                     /locus_tag="ACHK3A_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO8739528.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            28333..29034
                     /gene="hda"
                     /locus_tag="ACHK3A_001421"
     CDS             28333..29034
                     /gene="hda"
                     /locus_tag="ACHK3A_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO8739529.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(29140..29499)
                     /gene="arsC"
                     /locus_tag="ACHK3A_001422"
     CDS             complement(29140..29499)
                     /gene="arsC"
                     /locus_tag="ACHK3A_001422"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO8739530.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(29520..30983)
                     /gene="bepA"
                     /locus_tag="ACHK3A_001423"
     CDS             complement(29520..30983)
                     /gene="bepA"
                     /locus_tag="ACHK3A_001423"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO8739531.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            31196..32257
                     /gene="yfgO"
                     /locus_tag="ACHK3A_001424"
     CDS             31196..32257
                     /gene="yfgO"
                     /locus_tag="ACHK3A_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO8739532.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(32356..32826)
                     /gene="bcp"
                     /locus_tag="ACHK3A_001425"
     CDS             complement(32356..32826)
                     /gene="bcp"
                     /locus_tag="ACHK3A_001425"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO8739533.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(32826..33398)
                     /gene="gcvR"
                     /locus_tag="ACHK3A_001426"
     CDS             complement(32826..33398)
                     /gene="gcvR"
                     /locus_tag="ACHK3A_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO8739534.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            33544..34422
                     /gene="dapA"
                     /locus_tag="ACHK3A_001427"
     CDS             33544..34422
                     /gene="dapA"
                     /locus_tag="ACHK3A_001427"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO8739535.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASMKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            34439..35473
                     /gene="bamC"
                     /locus_tag="ACHK3A_001428"
     CDS             34439..35473
                     /gene="bamC"
                     /locus_tag="ACHK3A_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO8739536.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            35686..36399
                     /gene="purC"
                     /locus_tag="ACHK3A_001429"
     CDS             35686..36399
                     /gene="purC"
                     /locus_tag="ACHK3A_001429"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO8739537.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            36567..37430
                     /gene="ypfJ"
                     /locus_tag="ACHK3A_001430"
     CDS             36567..37430
                     /gene="ypfJ"
                     /locus_tag="ACHK3A_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO8739538.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            37445..39460
                     /gene="tmcA"
                     /locus_tag="ACHK3A_001431"
     CDS             37445..39460
                     /gene="tmcA"
                     /locus_tag="ACHK3A_001431"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO8739539.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATYVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENAIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDIVLSADDWLELAGFAFAHRPLLTSLGCLMRLLQTSEMALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALYALDDVRTERLRDRITQWQFFH"
     gene            39534..40232
                     /gene="ypfH"
                     /locus_tag="ACHK3A_001432"
     CDS             39534..40232
                     /gene="ypfH"
                     /locus_tag="ACHK3A_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8739540.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     TATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(40313..40513)
                     /gene="ypfN"
                     /locus_tag="ACHK3A_001433"
     CDS             complement(40313..40513)
                     /gene="ypfN"
                     /locus_tag="ACHK3A_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO8739541.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVNHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(40541..41668)
                     /gene="dapE"
                     /locus_tag="ACHK3A_001434"
     CDS             complement(40541..41668)
                     /gene="dapE"
                     /locus_tag="ACHK3A_001434"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO8739542.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(41672..42028)
                     /gene="yffB"
                     /locus_tag="ACHK3A_001435"
     CDS             complement(41672..42028)
                     /gene="yffB"
                     /locus_tag="ACHK3A_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO8739543.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT        10183 a        10413 c        11083 g        10365 t
ORIGIN      
        1 atacaatatt aaagcttatt tttgactgta tcattgtcac agggaaagtg catgctgcga
       61 tggaaacgct gtattattct aacatttatc tctggtgctg ctttcgcggc gccagagata
      121 aatgttaagc aaaacgaatc gttacctgat ttaggtagcc aggcagcaca gcaggatgaa
      181 caaaccaaca agggtaaatc gctgaaagag cgcggagccg attacatcat caactccgcc
      241 acgcaagggt ttgaaaactt gacccctgag gcgctggaat ctcaggccag aagctatctg
      301 caaagtcaaa tcacctcaac cgcacaatct tatattgaag acactctctc tccctacggt
      361 aaggtccgtt cgaacctctc cattggccag ggcggcgatc tggatggcag ttccatcgat
      421 tattttgttc cctggtacga taatcaaacc actgtttatt tcagtcaatt ttctgcgcag
      481 cgaaaagaag atcgtacaat cgggaatatt ggtcttgggg taagatataa ttttgatgaa
      541 tggttattgg gcggaaatat attttatgat tatgatttta cccgaggaca tcgccgttta
      601 ggtttaggcg gcgaagcgtg gactgattat ttaaaattct ctggcaacta ttatcatccg
      661 ctttctgact ggaaagactc cgaagatttc gatttttatg aggagcgtcc tgcgcgcggc
      721 tgggatattc gcgccgaagc ctggttacct tcttatccgc aactgggggg caaagttgtc
      781 ttcgagcaat attacggcga tgaagttgcc ctttttggta cggataattt ggagaaagat
      841 ccctacgcgg taacacttgg actgaattat caaccggtgc cattactaac cgttggggcg
      901 gactataaag cggggaccgg agataacagc gatgtcagca ttaatgccac tcttaattat
      961 cagttcggcg ttccgctaaa agatcaattg gatagcgata aagtgaaagc ggctcactcg
     1021 ttgatgggca gtcgtcatga ttttgttgag cgtaataact ttattgttct ggaatacaaa
     1081 gaaaaagatc cgcttgatgt caccctgtgg ttaaaagcgg atgccaccaa cgaacacccc
     1141 gagtgtgtca ttaaagacac gcctgaagcg gcggtcgggc tggaaaaatg taagtggacc
     1201 attaacgcgc ttatcaacca ccactacaaa attgtcgctg cgtcatggca ggcgaaaaac
     1261 aatgctgccc gcacgctggt tatgccggtt atcaaagaga ataccctgac agagggtaac
     1321 aataaccact ggaacctggt gctgcccgcc tggcagtaca gctctgacaa agccgaacaa
     1381 gaaaaactca atacctggcg agtacgtctg gcgctggaag atgaaaaggg caaccgacag
     1441 aactctggtg tggtggaaat caccgttcag caggaccgta aaattgagtt gattgttaat
     1501 aacatcgcga acgtgccgga agagaacaac cacagccacg aagccagcgc ccaggcagat
     1561 ggcgttgatg gtgtggtgat ggatctcgat gtaaccgaca gctttggcga taacaccgac
     1621 cgcaacggcg atgcgctgcc ggaagataac ctcacgccgc ggctttacga cgcgcaggac
     1681 aaacgagtga cgttaaccaa caagccttgc tcgaccgaca acccctgcgt ttttatcgct
     1741 aaacaagata aagaaaaggg tactgtcacc ctctccagta ccttacccgg cacctatcgc
     1801 tggaaagcaa aagctgcgcc ctacgacgac agtaactatg tggatgtcac tttcctcggg
     1861 gcagaaattg gtgggctaaa tgcatttatc tatcgtgtgg gggcggctaa acccagcaac
     1921 ctgataggta aagataaaga accgctgccg ctcaacaaca cttatcgatt tgttttgtgg
     1981 cgtgacagca ataaagacgg tgtcttccag caggtcgaaa aactgactga cgaagagatg
     2041 gcgcagtacg actacaagtg ggaatttacc ggcaagagca tcaatggtga agtgggtgca
     2101 caggcgaaca cttccaatga ggacattgtg attccggcca ctaaccgcga agcagcgcaa
     2161 acctatggcg cacaagcggg agatggcttg cagggatacg gtttacgcgt gctgtatacc
     2221 aaaaaataag attggcgtaa ggccagtcgg ccagtcgctg aaggttcatt aaggagagca
     2281 tcaatgaagc aggataaaag acgcggtctg acccggatcg cattggcgct ggcactggca
     2341 ggttattgtg tggcacctgt ggcgctggcg gaagaaagcg cctgggtcga cagcggcgaa
     2401 accaatattt tccagggaac cattccgtgg ctctattcgg aagggggaag cgctacgaca
     2461 gatgccgacc atgtaacgtt gacttctgat ctaaaaggcg ctcgcccgac aggcagtgaa
     2521 acggacaagc gtctttactc gggtgataaa ttaaccgtaa gttgggaaat tggtgatacc
     2581 gaaggcgacg tggatcttgg gggattgggc gataacgcaa agactatcga tactatccgc
     2641 tggatgagct acaaggacgc tcaggggggc gatccgaaag agctggcaac gaaggtgacc
     2701 agttacactc ttaccgatga cgaccgtggt cgttatatcg gtattgaaat tacgccgacc
     2761 acgcagaccg gtacgccaaa cgtcgggact gcgctgcatc tttatgacat ttccactgcc
     2821 agcggcggcg gaagcgacag cgataacgtt gcgccagggc cggtggttaa ccagaacctg
     2881 aaagtcgcca tctttgttga tggtaccagt atcaacctta tcaacggcag cacgccaatc
     2941 gaacttggca aaacctacgt ggccaaactg tactcggatg agaacaaaaa cggcaagttt
     3001 gatgcgggta ccgatgctga cgttaccgcc aattatgact tccgttgggt actttctggc
     3061 agcagccagc agctgggcac ttcaggtggt atcgttaact caagcttcga taataacaat
     3121 ctggccatcc ctgcgaccaa cgacgaagcc agaaccaacc ttaacggtcc tgcgcgcgat
     3181 ggtaaagagg ctcttaccat tccgacaaac ggcgacgggg tacagggtta caaacttcac
     3241 attatttaca aacacaaata acgcgtactc agcagcattc ccgcaaggga atgctgtttc
     3301 cgcatatttt gcttaatccc ggattaagca aaatatgttg atgcgacaag cgtccgttta
     3361 acaaccggtg gagtttctgt catgtccctg accctgaata aaatccttgt gttatgcgca
     3421 ttgctgataa gcgcaatgct gcctgggtgg tcatgggcag aatctgcatg gcaagactct
     3481 tcagatacgg tcggcgaatt taacggtacc gtgcccaccg ccgacagtgc cagcataccc
     3541 gtctaccagg gcagtgtctt tctggatcct gcaaagacgc atgaggtagc gtttaccgct
     3601 aaaccgagtg aattcagcgc ggatgttagc gtctctaaat tgctcgtcac caacccgcaa
     3661 gacagagaag gcgatatcat tgccacacca cgttgggaaa accaaacgcc accgacagta
     3721 tctttagttt gggcggatgc cgcgacgccg gacaccttac tggacccaca gccagtcgca
     3781 gatcgctcat tttgcgcaca gggccttgcc gggcgttcgc tgatcgcctg ggcgcagcca
     3841 gacccccagc agaccatgcc gctgttatat ctacttacct cgacgggcta tccctatgaa
     3901 agtgttctga cccttgccga tcagaaagtg acgctgaaga ttgcgcccgc gcagggtgac
     3961 cttatttccg tcagcgccgc gggctatgac gaatcctccg gtgcggcgaa aacgacggtt
     4021 ggcggcagta tcacgttaac ggtcaccacc aaagattgcg tcggtaatgt tgtggggaat
     4081 attccttttg ttatcaaacg caaagatgct gaaaaccgcc agggtgtagt gaacaatact
     4141 gcgccggtaa aactgggcac gaccgaatta accaccaccg cgacggaata tcgtggtact
     4201 accgatgcga atggtgttgc cacggttacg gtcacccagg ccaacggccc tggtgtaaaa
     4261 acgccgttgg tcgcgagcct cgcgggtatt gctcagacca gtgaaacggc agtgatcttt
     4321 accgtcctca ccagcccgga tgtcccgcag gccacaatgt ggggtcatat gcccgatacg
     4381 ctgaaggccc gtgattacac ttttagtcga ccaaaactgg cggcggaagt cgataatgaa
     4441 gacggtacgg ttaacgatca caatgaaacc tggtccacct ttacctggag tggtgcggat
     4501 aaacattgcg acattttgcc cggaatgcgt cagttcggtg cgctggcgac ggtggtgcca
     4561 acgtcagtgc aggatgtggc tggttggccg atgcagggta actactactg gtcatcactg
     4621 gcggggatga gcgggcagca ctatgcggcg gatgtttcga accgcagcga agcgcaaaaa
     4681 ccggacgata cgacgtttat tgtgagctgt gtcgataaag aagcgcccga tgttgaaccg
     4741 aagctggtac tgacgccggg cagttatgat tcgacgataa aagcgatgaa agtaaaggtg
     4801 ggggaagagg cttctttacg cctgacgatt accgatagca agaacaacga tcagccgctg
     4861 gcttactact acttttcgct gcatctggat gacggtgtta accgtaagaa tcaaactgac
     4921 gctgcgtggg agacgcaccc ggtacaaatt gatggtggca gtaacgttcg caaagttgac
     4981 gcgcatacct atgaagggat cactgatgct aacggagagg cctctctgac gctgacccag
     5041 ccaggcggcg tgggggtgaa aacacacatc accgcgaaaa tgcgcagtga ttataccgcc
     5101 tcggatgaga aagacgttat ttttacggtg atcaccagcc ctgattccga caaagcgcgg
     5161 atgtggggac atatgttagg cattattgaa gcgaataata tctttaaacg cccacggctg
     5221 gcggatgaaa ccgacaacga actgggagca gtgcgggaaa ataacgaaga ctgggcgctg
     5281 tttgatcaga atagcagtat gcaggctgag tgtggtttag gtcatattcc gagccaaagc
     5341 tcattgcaca gtttgtttgc cgcacatcca gccaatgtca tcggcaccga atatggctgg
     5401 ccaacgttgc aaaaagcgta tcttagtgcg gtggaagaga cgtcgcacgc gtcggtgaac
     5461 ctggccacgg gcaacatcga cacctattcc ggctttaagc aaaactacct ttcttgttcg
     5521 ggcaatgaga tggtcgccca gattgctgcg accaccgatc atgatgtttc cacaagcagc
     5581 agagcgcagg ctaaagtggg tgaaacgatt accatgacgg tacgcacctt taatgccttg
     5641 aataatgcgc ccgtgccgta taccgcgttc accatcacta aagatatggg gaaaaatcgt
     5701 caggggcaga ctacaggttt tgatgatcca acccgcggcg caatcgagat gaatggcaca
     5761 ctctacggaa catcgcaacc gtctttagtc tatgccggta ctacggatgc gcagggtttt
     5821 gctactgtgg aaattaagca accgcaaggt gtgggcttag cgacgccgct gaatattgcg
     5881 cctgtaaact cctatattcc caatactgtt aattacaatg tgattttcac tacgctcacc
     5941 agtccggatg cggttggcgc gcagatgtgg ggtcatatgg atgagacgat taccgtcgat
     6001 gcattaacct ttgcccgacc ccggctggct gcggaagtgt ccagcccgga cggcacatta
     6061 acggaaaata acgaagtgtg gtcgcgcgtt agccaggcca acacttccag caccagtaaa
     6121 ggtggctgtg gtgccaatat gctcccacgc cgttcccagc ttaacgcgct ctatgatgct
     6181 aacagcggta acgccgttca aacggtacac ggttggccaa cgcagcgtca gccctactgg
     6241 agcagttcgc ccgcggatca ggtgccgcat tactacacca tagccctcaa cgatggcgcg
     6301 cggaccgttg gcggtagcac ggcggtttat gtcagttgcc tgacgacggc taacaatcct
     6361 gccagcagca ttacgctgga agtggtggac ccggcgcagt ggaacgcggc agcaaatgcg
     6421 gcgaagctga aaaaaggcga aacgctacag gtgaaggtga cggtaaaaga tgctcagggc
     6481 aatccattgg gtgatatgcc ttttacgctg aagcgtggcg atggttatac ccgatcaaac
     6541 gaaaaacaca ttgccggtgg cggtgatgcg ctggttgcgc cggttgtgat caatagtggc
     6601 ctggcggatg aaacttcgct caatgatacc gccgcagcat acagcgcgat gaccggcagc
     6661 gatggcacta aaattctcac tattacccgc ccggataccc atggcacaaa aacgtcgctg
     6721 accgccgcgc tctattcaga cacaacgaaa aaagcgaccc tcgataccat ttttaccgtt
     6781 gtcaccagcc cggacagcga taaggcgaaa atgtgggggc atatgcctga aacagtgacg
     6841 gctgcagatg gaacggtctt taaacgccca ttattgttga aagagttatc cagtacgtcg
     6901 ggtcgaacag cgattgcaga agagaatgaa gactgggcgc aatttaccca aacccaggct
     6961 ataagcacat cctctaatgg ttgtggtagt gaatatgttc cctcccaggc cgggctggag
     7021 tcattgtatg aagccaaccg cggcaatgcg atgaaaacgg ttcagggctg gcccgtcgcc
     7081 tcaagttacc tttccagcac gacaggtagt agtagcctgg aacagcgtga cttcaaggcc
     7141 gtcaatctca gttcaggcac cagttcgata attccatctg ccacaaaaga actgcttacc
     7201 tgccagacga cgccgattgt taaggcaagc caaattgttc ttgaagctgc agaccccaca
     7261 aaatttgaca gtacgaataa tgttgtcaaa gcgaaaaaag gcgaagaagc cgtattacga
     7321 gtgaccacta aagatgcgca gggtaaccct gtgggaaata ccgcatttac cctcaaacga
     7381 aacaccagcg tgaacagggc caatgtgtca actacgacgt cgattgcttc gctggcggta
     7441 acggatgcct ggggaaatac tcaagatgat tttctttcaa cgaccctggt tatttatggc
     7501 gtgacggggg ctgatggcat caccactttc acattgaagc aagaccagac taccgggcta
     7561 aaaacagaac tcacggcagc gttggattcc tccagcagca ctaaatcgac gcttcccgtt
     7621 gtctttacgg tgcttaccag cccggattcg ccaaaagcca aattctgggg gcatatggct
     7681 gaaacggcga caggcgatga cggtcttatt tatcgaaggc ctttactgag ggacgagaat
     7741 agtgctacca ccagtatcgg taccctggtg gaagaggggg aggcctggtc tacgttcccg
     7801 tcgggccagg ctaacgatac ctcgataaat ggttgtggcg cggagtatgt tccaactgac
     7861 aatgaactta gggccattta tgcgcaccag gggagtagcg cattacatga tgctattggc
     7921 tggccagtgt cgaggttcta tatctcgaat accgttgcag atacgttcac tcaaaccttt
     7981 acgtatgatg tcgtttcgct gaagacagga gatgaaacgc agatgccctc atctggtggt
     8041 gcattactga gctgccggac cacgcctgtc gccgttgcca gtcagatcat cgttgaggcg
     8101 aatgatacgg cgcaatttgt caaagttgac gacaccttga gtgcgttgaa agtgaaaaaa
     8161 ggcgaagacg cggtgattcg cgtggtgact aaaaatgcgc agggaaatag cgtgcccaat
     8221 gtccccttta ttctcagacg tgaaggctcg aagaaccgtc aaaatgccga gatgataaat
     8281 aagtcgatta cggtaattaa cgcggcaggc gcttctgcga ggatgaatag ttcttcaagc
     8341 ctgctttatg gggtgactgg cgcggatggg actacctcct ttacggtaaa acaagatgat
     8401 tctatgggcc tggtaaccaa tatgtacgcc cagttgtatc agccgacgat agaatccaac
     8461 aaattaccgg tcatgtttac cgtcataacc agcccggata cgccactggc aagttactgg
     8521 gggcatatgc ctgaaacgtt tactacccgg tcgggaattg cctttaagcg gccattgctt
     8581 actgccgagc atccagcggg gcagtcaacc atggctaata atgaatcatg gctttctttg
     8641 aatacggcag caaaaaatga tgtcagcaaa tcagactgcg gcgaacctta tcaaccgtta
     8701 ttaagcgaat ttcaagagtt atacagtgaa catcctaatg gtgcgatagg gaccgattta
     8761 ggccttccgc tgactaacac ctggtgggct tacgataaaa ttgcctacgc gaacgtctgg
     8821 tacgatcagt ctattaattt atcgaatgga tcaagctcca gggcattatc aaacaccgtg
     8881 gcatttgtgt cctgtctggt aaaccctcat gcagtggcag cttctataga gatgacctcc
     8941 acggcactgg atgcggagaa aacggccagc aacgatggtc gccctagtgc aacggcggcg
     9001 aaaggtacgg cgatccggat gacggtgatt gtgcgggata gcgcaggaaa tctgttgcca
     9061 ggcgcaaact ttaatctcat acgcgggact gcgttggatc gtgcaaaaaa caggctagat
     9121 tctacgtatg atgatcttac tatcgtgccg gtcacccctg ccggagtaaa catgtcactc
     9181 tataataatg gggcacaggc acttctgacg acaggaagtg atggtaaagc gacttttgat
     9241 gtgacgcaaa acgagactta tgggctggca actccgctca ctgcaacatt gatgcgcgac
     9301 accacaaaaa gtgccacgat ggatgttatt ttcactgtga taaccagtcc gaactcaccg
     9361 aaagcaaaat actggggtca tatgcccgac acgtttacca gtagggcggg ggtgaccttt
     9421 aaacgaccgc tactggcagc agaagcgacc ttggggtcct cggtaagtaa taataatgag
     9481 tcgtggtcat acctttttta taccaataaa gttacgccgg attgcccggt tgagtatcag
     9541 ccgcgtctca atgagttaca ggggctatat aatgatcatc caggcgggac aatactgaca
     9601 gatttggggt tgcctataac tgccggaagt ggaaattggt ggacttatga aatgagcacc
     9661 actgacgcgt tgacgtggta ttacggcgtc attaatttga aaacgggaca atcgacgacg
     9721 actattaatg gatacgcttt aatgctctgt ctgacgcagc cacattcggc accagcaagc
     9781 cttacgctgt catcgactgc ctatgatgag ggccgaactg ccagcaatgg tggtaccccc
     9841 acgtcatctg tgaaaaaagg tgagatgctt cccatcgtgg tgacgatcaa agatgccaat
     9901 ggtaatcctg ttggcggcga aggtgttacg ctcaagcgcg tgcaggcgaa aagccgttca
     9961 gggatctctg tatcctccaa taccgtagat gatctcattt tagatgaagt caccccgaca
    10021 tcggcgcgta tctccttcaa ccagaatacg tcggcatgga gcggcttcac cggcagtgat
    10081 ggcacgataa cgttcaacgt tacccaaaat aacaccgtcg gtctggtaac gccgtttaca
    10141 gcttcattgg cgcgaaatcc gcaggttacg gctaatcagg acctgatatt cacggtagtg
    10201 accagcccgg attctgcgaa ggctaactac tgggggcata tgccagcaac cttgacggca
    10261 gtgaatggtg cggtatttga acgcccgaaa ttgtggagtg aactgacatc gacttctggc
    10321 gtcggcaaga tcaataacaa taatgaagac tggccgtact ttaccccaac acagaaatca
    10381 gatgcgagtg tgagcccttg tgaagtagca aggcagccac tattcaatga tttaagttcg
    10441 ttgtctgcgc gttatccgaa taacacgttt gtcactgaaa ctggctggcc tgcgtattac
    10501 acttggtggg cagaggataa atccgccgat ggtaaagacc agtcggttga tttgagaaat
    10561 ggcacgctgt atacgggttc aacaaaatca tttcagcctt gcctggctaa cgcgcgttca
    10621 acggtttcca gcgtcacact tacctcaaca gcgtttgatg cagccactca ggcggcgaaa
    10681 gtgaaaaaag gcgaagccat gcccgttacc gtgacggtga aagatagcgc cgggaatacg
    10741 gtgccgaatg tggaatttac tttgaaacgc ggtgaggcat cgccgcgtaa cgctggggca
    10801 acgttgtatg gtgatgtggt ggcgatggac gatctcatcg ttcagccgtt atctggttcg
    10861 gctgttacgc tgagtgaaag cggtaatacc attagtggca tgacaggcgc ggatgggacg
    10921 gcaagtttta cgctccgcca ggataatacc cctggttata aaacgccact gacggttact
    10981 ctggctaatt acgcttccgc gacagacacg ctggacgcta tttttacggt accaaccagc
    11041 ccaaatgttt cctcggcgca cttttgggga catatggcag acacggtagt ggtgaatggt
    11101 aaatcgctac atcgaccgtt gttaacgaca gaattgccgt ccggggctaa tcccgtatcc
    11161 tcaccgatta ttaactacga aaactgggct tctgcacata tcattgatgc cagtaaatgg
    11221 gatatcgcaa ggcagtgtgg cagcatagaa aatgcaccga catacaatga attagagttg
    11281 ttgcatactg tttttaattc actgggatgg ccgtcttcac ccagttttcc ttatttgtcc
    11341 tctcaacaat gtgggatgga cgaaggtacc ggagcacagg attgtagtat cacgctaata
    11401 aataaaccgg gtctggtgac ctgcttccag taaccagcat ggcggatctg gcgtggcctg
    11461 ggagttattg ccggatgcga tgctggcgcc tcttatccgg tctacggatt agacgttttt
    11521 ttacgtgatt tgcgggtttt tgttacggcg ctgacttcac taatcaccac accatctccc
    11581 agtcgtgtgg tcagtgtctc acccactttc acttgcttaa cctgttttaa caccgcgcca
    11641 tcagcggcgc tggtgacgct ataaccgcgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgtgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcagggtt
    11761 tctgctaaac gatattccag ttgttgaatt cgcgtctgtg cgcggtgaat acgcggctgc
    11821 ggattttgct ggtttagcct ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgcttt tgcagacgtt cgagcatagt ttgctgacgt
    11941 gccagccgga gctgcggatg ctgttgctgc aagcgatgat gaatctgcgt aaaacgacgc
    12001 gtgcggttgg cgagataata atccatcgcc atttccagcc gttgatgggt cgattgcacc
    12061 tgacgtagca attcttgctg atttcggctc accacttcag cggcggctga cggcgttggc
    12121 gcacgtagat cagcaacaaa gtcagcaatt gtcacatcgg tttcatgccc gacggcgctg
    12181 actatcggaa tgcgactggc aaaaatcgcc cgcgccacac gctcatcatt aaaactccat
    12241 aaatcttcca gcgagccacc gccacgcccg acgattaata cgtcgcactc gttgcgaaga
    12301 ttcgccagct caatggcgcg aacgatttgc cctggtgcgt catcgccctg aacggcagtg
    12361 ggatagatta tcactggcag agacggatcg cggcgtttca acacatgcaa aatatcgtgc
    12421 agtgcggcac ctgtttttga ggtaatcacc ccaacgcagt gcgcagggga ggggagtggt
    12481 tttttaagtt gctgatcgaa caaaccttca gcctgcaact tcgctttaag ctgttcgtac
    12541 ttctgttgca gtaaaccttc accggcgggc tgcatacttt cgacaataat ttgataatcg
    12601 ccgcgcggtt catacagcgt aatgttggcg cgaactaaaa cctgttgccc atgctgcggg
    12661 cggaaggtga cccggcgatt gctgttgcgg aacatcgcgc agcgcacctg ggcgttatcg
    12721 tctttaagcg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaca cctgccccat ctcatgctca agcagcagac gaaccgtttg attcaggcgg
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcga tagtaacccg ccctccgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgcacatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgacccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggc tttgcgggct acccggtcgt taccgaagaa aacgaactgg tcggcatcat
    13441 caccggtcgt gacgtgcgtt ttgtgaccga tctgaatcaa ccggttagcg tttacatgac
    13501 gccaaaagag cgtctggtaa ccgtgcgtga aggcgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagatt tccagaaagc agaacgtaaa ccgaacgcct gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgcagcggt tggtgctggc gctggtaacg aagagcgtgt
    13741 tgatgcgctg gttgccgcag gcgttgacgt tctgctgatc gactcctctc acggtcactc
    13801 tgaaggtgtt ctgcagcgta tccgtgaaac tcgcgctaaa tatcctgacc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc ccgcgcgctg gcagaagccg gttgcagtgc
    13921 cgttaaagtg ggtatcggcc ctggttctat ctgtacgacg cgtatcgtga ctggtgtcgg
    13981 tgttccgcag atcaccgccg ttgctgatgc agtagaagcc ctggaaggta ctggcattcc
    14041 ggttatcgct gacggcggta tccgtttctc aggcgatatc gccaaagcta tcgccgcagg
    14101 cgcaagcgcg gtgatggtgg gttccatgct ggcaggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttacaaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcttctgacc gttacttcca gagcgacaac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tggcctataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatggggct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 cttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttcccagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga actgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caacccaagc ggcattattc tttccggcgg cccggaaagc
    14761 accaccgaag aaaacagccc gcgtgcgccg cagtatgttt ttgaagcagg cgtaccggta
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gttgaagtcg taaacgacag cgcactggtt
    14941 cgcggtattg aagatgctct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgttgcaccg cgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 ctgaatattg ttcacgtacc ggcagaagat cgcttcttgt cagcgctggc tggcgaaaat
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ctatctaccc tgacgttatc
    15601 gaatctgctg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggt
    15661 ggcctgccga aagagatgaa gatgggcttg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctaccgtatg acatgttgta ccgtcacccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggagaag tgaagaaaga gtattgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcattcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cacatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaatg gtatttcccg cgtggtgtat gacatcagcg gcaagccacc agcaactatt
    16141 gagtgggaat gatttgaccc tgcactatga atgaacaaaa ccctctgtta ctacagaggg
    16201 ttttttatcc taaagaatta taggtttgaa gttactaaca tcgtttagtt aaaccagcta
    16261 tctgatttat tctcttctgc tttgcccacg cttttcatca gatcgcgacc gccttcagtc
    16321 atattcctgt ttgcgtcagc ttcagattgc accacatcgg tttgcgcagc tttgtgcttc
    16381 agttcctgat cgataaattc gttttctcgc ttaacgcggg cttcttcttt cgccagcgcc
    16441 agtttttgtt tctgaatctc taagctgcgt agctcatctt cataactttg atcgcgtttt
    16501 ttgtccgcag tggcttcggc gtccagttta tcctgacgag ctttcttatt cgccgctgcc
    16561 gttgccgctc ttttattagc cgcggcctgg gcgtttgcgc gacgttgctt ctcttgctgg
    16621 atttccctgt tgcgctccgc gacccattcg tcatgctgcc tttgctcttc atttttacct
    16681 tgctgttccg cttctgcgac agccgagagt tgatcctgca atgatgaggc gatagccgga
    16741 tagcttaagg aggctaagat ggcgcaaaga aaaactttct tcatgactcc tcctgattat
    16801 tagctctttt caggacattt ggtatttggc tgaatacgtg tttcgttata cgtcgtggta
    16861 ataacaacgg ctaaacctgt cgtaaactgg cactctttac ccacctgggt ggaggtatac
    16921 actttggtgc cttccttata tgttaaagaa acaccttcca ctaaggtttt atcattcacc
    16981 atagaacccg ctgccgcgcc tacagctccg ccgccaactg cacctgccgt cgttccggaa
    17041 ttgctgccag acccgacgtt gtggccgata acaccgccag cgacagcgcc aataagcgcg
    17101 ccgaaggctt gtgcgttccg tttattttgg gcgttgtcta cggcaacttt tgcgggaaga
    17161 atggaaataa tattaacggt tttagtttct tgtttggtat tcagttgatc ggtttgataa
    17221 acatcggcgg catgatcatc agcatttgac tggcatcctg ccagagtgaa tgacgctaac
    17281 attgccacag gcagaagaca ttttttaaat ttcatcacta ttccttgtta tacacattac
    17341 gaaaaataag acatttaaga tcatcaaaag aggaaagtaa atgtattgcg tataaaggaa
    17401 tagattaaat ttattaaaat tcatgcggga aataatttat aaaaatacat catcacaaca
    17461 tatgtattaa tgatgtattt atttcgcagt cgaacaggat aaacatcagt caggacaaaa
    17521 acgtaaaatt taagatgaaa aaatatctgc cgctttgata atcccaaaga gcaaaagcat
    17581 ccgacagcga ggtgccgtta tgttctgtga gagtggagaa ggtaaccgct aacgttgtac
    17641 tggtgattcg acggagagtg tggattgctg agcttctttt ttgctctgat ggtgctgcca
    17701 ggcaccgacg gacgagtaga taaaacggcc aaagaagaag ataaagctga tgagcagtac
    17761 gatacgggtc atgcgactgt taaatcggtg tcgttttcgc atactggttg cctgactcac
    17821 aaaaggttcc ttgaagtatg tcccacgccg tggacggtac ttacattaag gcacaacagg
    17881 acaaaatggt caattcttcg ttatgtaaaa aagcgtcagt ggatacatat tttaatgtta
    17941 tggaagttaa tttaattatt tacaatgatt atgtaataat gatgagttaa catgataaac
    18001 gtaataattc attaatcagg gttattttgt ttgatatata tcaattggaa ctttcatatg
    18061 acacttagaa tcattgctct ctatagtgat gaataatcat cattcgaagt caggtgggat
    18121 gtctgtctga atacacctcc ttcaggatgt gggggattct gctgagcatc tatgaaactg
    18181 aatgcaactt atataaaaat acgtgataaa tggtgggggc ttccgctgtt cctgccttct
    18241 ttaatcttgc ccattttcgc ccacattaat actttcgcgc atatttcttc cggtgaagtt
    18301 tttctctttt atctgccact ggcactgatg atcagcatga tgatgttttt cagctgggcg
    18361 gcattgccag ggatcgcctt agggattttt gtccgcaaat atgcagagct gggtttttac
    18421 gaaacgctct cattaacggc taattttatt atcattatca ttctctgttg gggcggttac
    18481 agggttttta ctccccggcg taacaacgtt tcacatggtg atagccgttt aatttcccag
    18541 cgactattct ggcagattgt gtttcctgca acgctgtttc tgatactttt ccagtttgct
    18601 gcgtttgttg gattactggc gagcagagaa aatctggtcg gcgtcatgcc ctttaacctc
    18661 gggaccttaa tcaattatca ggccttgctg gtgggtaatc tgattggtgt cccgctgtgc
    18721 tacttcatca ttcgggtggt gcgaaatccg ttttatttac gtagctatta ttcgcaatta
    18781 aaacagcagg ttgatgccaa agttaccaaa aaagagttcg caatctggct actggcatta
    18841 ggtgctttac tattgctgtt atgcatgccg ttaaatgaaa aaagcacgat ttttagcacc
    18901 aattacacct tgtcattatt gctgcccctg atgatgtggg gagcgatgcg ctatggttat
    18961 aagctgattt cattgctctg ggcggtcgtg ttgatgatca gcatccacag ctatcaaaat
    19021 tacattccca tttatcctgg ctataccacg cagctgacca taacctcctc cagttatctg
    19081 gtattctctt ttattgtcaa ttatatggct gtactggcaa cccgtcagcg agcggtagtc
    19141 agacgcattc agcggcttgc gtatgtggac ccggtggttc atctgccaaa tgttcgcgcc
    19201 ctgaatcgcg cgttacgtga tgccccctgg tctgcgcttt gttatttacg catccctggc
    19261 atggaaatgc tggttaagaa ttatggcatc atgctgcgga ttcaatacaa gcaaaaactt
    19321 tctcactggc tgtcaccatt gctggaaccg ggtgaagatg tttatcagct ttcgggtaac
    19381 gatctcgcgc tgcggctgaa tacagaatcg caccaggagc gcattaccgc actggatagc
    19441 catctcaagc aatttcgttt cttttgggat ggaatgccga tgcaaccgca gattggcgtc
    19501 agttactgct atgtgcgctc gccagtgaat catatctacc tgctgctggg agagctaaat
    19561 acagtggccg aactttctat cgtgaccaac gccccggaaa atatgcagcg tcgcggagca
    19621 atgtatttgc aacgcgaatt gaaagataaa gtcgcgatga tgaatcggct acagcgggcg
    19681 ctggaacaca accatttttt cctgatggcc cagccgatta ccggtatgcg tggtgatgtc
    19741 taccatgaaa ttcttctgcg catgaaaggt gagaatgatg aactgatcag ccccgatagc
    19801 ttcttaccgg tcgcgcacga atttggttta tcgtcgagta tcgacatgtg ggtcattgag
    19861 catacgctgc aatttatggc tgaaaacaga gcgaagatgc ccgctcaccg ttttgcgatt
    19921 aatctgtcgc caacctcggt atgtcaggct cgttttcctg ttgaaatcag tcagctgctg
    19981 gctaaatatc agattgaagc gtggcaactt atttttgaag tcaccgaaag taatgctctg
    20041 accaatgtta agcaggcgca aatcaccttg cagcatcttc aggaattagg ctgccagatt
    20101 gcgattgatg atttcggcac cggctacgcc agctatgcgc ggcttaaaaa tgtgaatgcc
    20161 gatctgctta aaattgacgg cagttttatc cgcaatattg tgtcaaatag tctggattat
    20221 cagatagtgg cgtcgatttg ccacctggcg cgaatgaaga aaatgcgggt agtggcagag
    20281 tacgttgaaa acgaagagat ccgcgaggcg gtgctctctt tggggatcga ttatatgcag
    20341 ggttatctta ttggtaagcc gcaaccgtta attgatacgc tgaatgaaat cgaacccatt
    20401 cgcgaaagtg cctgaataat gcgggccgac atttctcgtc ggcccgcaaa gcattaagcg
    20461 gcgatttctg gtgtactttc ttcttcaatt ttcaaccgcc agccagccac gccttcccag
    20521 tattcttgct ccttttccag atcaagcagt accagcgcat tctgactaaa ccagtcatgc
    20581 ggaaaacgca gtgtccagtg actgtcatcg gtaatcagcg tcaatgttgg cggtgtggtg
    20641 gttgcctgac gttgattatt gagtaatacg ccaaggcgca atagctgaat cagtggcagg
    20701 aactgtttct tcttaaacaa ggtaaagcgc ggcagatcgt cgagcttaat cgctttacgg
    20761 tgatagcgca ccagcgtcgc catcatcaac tgctgttcct gattaaaacc cggcaagtcg
    20821 ctgttttgca gaatataagc ggagtggcga tgcaaaccgc tgtggttgat attcaacccg
    20881 acctcatgca gcatggcggc ccatcgcagt agcgcttcca gttgcggatg cgccagcttc
    20941 ggttgctgtt cccgccactg ttcgtacatt tgcatagtgg tatccagcac ccgtcgggcc
    21001 tgttcgctgt cgatgtgata ctggttggcg aggctgctgg cggtgcgact acgcacatcc
    21061 tgatgacgga aacgtccttc catttcatac agtacgcctt cgcgtaacgc cccgtcagaa
    21121 aggcgcagtt cacggatggc taaagcatca aacacaccgc ataaaatcgc cagtccagga
    21181 acgaagactg ttttccgttc ttcggaaaga cctggtaagc tcagcgatgc gaaattacgg
    21241 tgccgtaaaa cttcttttac cagtttttcc agacgttccg gggtaattat cccgtctttc
    21301 tcgcccattt ccattaacac ttcatgggcg gcttttattg taccggaagc gcccattgca
    21361 acattccagc cctgaattcg aaattgccag gttaaagttt ccagtttttg tgctgccgcc
    21421 atgcgagcgc gctgaaaatt ctctttattg atgaccccgc cagggaaata aagctgggca
    21481 aagctgacac aacccatccg gcggctttca acgagaatcg gttcgaaatt ttcaccaatc
    21541 accagttccg tagatccgcc gccaatatca ataaccagtt tgcgaccttt ctccggttgg
    21601 gtatgttcca cgcccataaa aatcagacgg gcttcttcat taccggaaat aatttcaatc
    21661 gggtagggaa tgaccttttc cgcgcgtttc agaaagtcag tggcgttcag cgcctgacgc
    21721 agtgtatggg tgccaactat acagacgctg gcaggagaaa acccttgcag ccgttcggca
    21781 aacagcgaca gacagtttaa accgcgcgtc attgcctctt cactcaacat attatctggc
    21841 cccaggccgt ccgccagatg cactcgctgt ttcaggcggc caataatctg catggcacca
    21901 tctaccacac gggctatgac catgtgaaaa ctgtttgaac caagatcgac cgcagcaaac
    21961 tcctgcggac gaggggattt atcgtgtatt ggcatagggt tattcaggtt gttcgagtga
    22021 tttgatgtag tcgtaaatcg ccaactgcgc ccgtacttta cggcgattgc cgcggggaac
    22081 gtagcgatta ctgagttctt tatcgatata acgtgctttg accgtatcgc tgaacaatat
    22141 gtcgatgatg tccagcaccc gctgcttcag gcgcggatcg agcagcggtg tcgccacttc
    22201 aatacgataa tcaatattgc gcgtcatcca gtcggcggaa gaaaggtaga cctttttatc
    22261 gccgccattt tcaaaaatat aaacccggtc atgttcaagg taacggtcaa caatactgat
    22321 ggcacgaatg ttgtcgctaa tgccttccag attggggatc agcgaacaca tgccgcgaac
    22381 cagcagatta accggtacgc cggagctgga ggccgcatac agacgatcaa ccaggccttt
    22441 atcgacaagg ttatttagct tcagggtgat accgctgggc agcccttgct gcgcgttggc
    22501 gatttcgcgg tccaccattt catacaacag gcggcgggag ttttgcggcg acaccattaa
    22561 ataatcaaat gtcaccggac ggtatgggtt ttcaataaag ttgaacaccc gccgtacttc
    22621 gttggttatg cgcgcatcgg cggtcagcaa cgaatagtca gtataaagac gcgcggtttt
    22681 ttcgttaaag ttcccggtcc cgatgtgtgc gtaacgcacc acttcaccgt tttctttacg
    22741 tgaaatcagg aacagtttgg cgtgaatttt cagccccggc gcagagaaga taacgtgcac
    22801 gcctgcttcg gtcaggcgct tcgcccagtg aatgttggct tcttcgtcga aacgcgcctg
    22861 taactcaacc accacggtga ctttcttacc gttgtgtgcg gcgtggatca tcgagtcgat
    22921 gatgcgtgaa tctttcgcca cgcggtaaat gttaatttta atcgccagta cgctcgggtc
    22981 gaacgaagcc tgacgcagca gttccagtac atgctcaaag gtatgataag gataatagag
    23041 caacacatcg cgttcgcgaa tagcatcaaa accattgcgg aactgggctt tatcaaacca
    23101 aatgtggcgt aaacgcggca gtggtttgtt caccagattg gctttgccga cattggggaa
    23161 attaataaag tctttaaaat tatgataacg accaccgggg acgatggagt cgtagcggga
    23221 aatagtcagt ttttcgcgta acacttcaac cagcgcattg ggcatatcgc gctgataaac
    23281 aaaacgcacc ggctcagcag ttaaacgctg cttgagactg gaagacatca actccatcag
    23341 gctggcttcc atctcatgca ctaaatcgta ttcggcatcg cgggtcatct tcattgaata
    23401 ggcattcagc gcgtcataat caaagaagcc tttgaaaata tcatcgaggc agtaacgcag
    23461 aatgttatcc agaagaatca tcggcttgcg tcgacgtggc gcttctggcg gtaaattcac
    23521 aaagcgcggc actttatctg atgggatctc cagcagcgcg taacggatgg tatcgccacg
    23581 gataatttcc accgccagat aggtgtaatc atctttcagg aactgcacta agtcagtgtc
    23641 aggattgatt aaaatcggcg taatgtgctg acgcagatac tgcttaaaat aatgacgcag
    23701 ccagttttgt tgattgacgg agagctggcg ttcattaatc aggaagatct ggttgcgcgc
    23761 catctccagc aacagctcgt tgtagaggcc gtcgaattcc tgatcggctt tcagcacccg
    23821 ggactgaatt ttgcccagta aatggcggga atgagagttg gaaccttgtt cttcgctaat
    23881 aatgatgcgt cgcttcagtt cagcgaagcg gactttatag aactcatcaa ggttattgga
    23941 atagatcccc aggaaacgca tcctttcaat cagcgggtta gatttgtccg ccgcttcctg
    24001 aagcacgcgt tcattgaacg ataaccaact gagctctttt tcgatgtata gcttttcctg
    24061 acccattacc acttttactc cgttttattc acgggacact gcctggatat tatggcgagc
    24121 aatattggca gatgtccaac tatgccagaa aagggggaaa aaaactgctg gagcttgcga
    24181 tttacaggtg aattgaaaaa cgccagcggc agagctggcg ctttaactac ggggcattac
    24241 tcgtcggcag cgtagccctg cggcggcaga cgttgaccat ccagccacgc ggcgttttcg
    24301 tgcattttca gacgaccatc ggcaaaccag ctaatcacca gtggataaat agcgtgttcc
    24361 tgggtttgca cgcgggcggt gatgtcatct tccgtatcac cagcaaatac cgggactttc
    24421 gcctgtaaaa taaccgggcc accgtccagt tcatcggtga cgaaatgcac cgatgtaccg
    24481 tgctcttcat cgccattttc cagcgcctga cgatgggtgt gtaatccggg atatttcggc
    24541 agcagagaag ggtgaatgtt cagcaaacgc ccggcatagt gggagacaaa cgccgggctg
    24601 agaatgcgca taaaaccagc cagcacgacc acatcgggtg cgtacatgtc gatttcatga
    24661 atcaactccc ggtcataggc ttcacgactg tcaaacgcgc tggcgatgag cgtatgcgtt
    24721 gcaatacccg cctggcgggc gcgttcaagg ccgaacgcgt cggccttatt gctgaaaact
    24781 gcccgtacgg tgcctttaat tttgttggtt ttgcaggcgt caataattgc ctgcaaatta
    24841 cttccgttgc cggaaataag caccacaata ttcattattc gataaccacg cgttgttcgg
    24901 aatcagaggc tttgatgata ccgattttcc acgcgttttc accgttggca ttgagcaggg
    24961 cgagagcttt gtccacttcc ggagcaggca gggcaataat cataccgacg ccgcagttga
    25021 aggtgcgata catttcatgg cgctcaacat tacctgccgt ttgcagccag ttgaacactt
    25081 ccggccactg ccaggaagat tcatcaatca ctgcctgggt attatctggc aatacgcgcg
    25141 gaatgttttc ccagaagccg ccgccggtca ggtgcgcaat ggcatgcaca tcgacctttt
    25201 caatcaattt cagcactgac ttcacgtaaa tgcgggtcgg tgccagcaga tgatcggcta
    25261 atggcttacc atcaagttcg gtggtttgcg gatcacaacc gctgacttca agaattttgc
    25321 gcaccagcga atagccattc gagtgcgggc cgctggagcc gagtgcaatc agcacatcgc
    25381 cgtcgctgac tttagatccg tcgatgattt ctgatttttc taccacacca acgcagaaac
    25441 cagcgacatc gtaatcttca ccgtgataca tccccggcat ttccgccgtt tcgccaccca
    25501 ccagtgaaca gcccgattgc agacaacctt ccgcaatgcc gctgatcacc gctgaagcgg
    25561 tatcaacatc cagttttccg gttgcgtaat agtcgaggaa aaacagcggt tccgcacctt
    25621 gcaccaccag gtcattaacg cacatggcga ccagatcaat accaatggtg tcgtgacgtt
    25681 ttaagtccat cgccagacgc agcttggtac ctacgccgtc agtgccggaa accagcacag
    25741 gttcacgata tttttgcggc aatgcacaca gcgcaccgaa gccgcccaga ccgcccatca
    25801 cttccggacg acgcgttttc ttcactacgc ctttgattct tccaaccaga gcattacccg
    25861 cgtcaatatc aacaccggca tctttgtagc taagagaggt tttatcggtc actgcttggg
    25921 tccccacgcg ttacttgcgg tagaaaaata aaattcggcg caattctaac agggaaagca
    25981 aacgtttgcg agactgcttt acacaacctt tttgcacgtc ttttccccag gcgcgcggcg
    26041 aaagaatact tgtactaggg caaaggatcg ttttcggatg gaataatctt cttccataac
    26101 catttgaata taaaataact ttatctcaaa ccgttatcat tttgactaaa gtcaacgaaa
    26161 ataatattgc cgccttgaag aaaggaggta taatccgtcg attttttttg tggctgcccc
    26221 tcaaaggaga aagagtatga agatcgtgga agtcaaacac ccactcgtca aacacaagct
    26281 gggactgatg cgtgagcaag atatcagcac caagcgcttt cgcgaactcg cttccgaagt
    26341 gggtagcctg ctgacttacg aagcgaccgc cgacctcgaa acggaaaaag tcactatcga
    26401 aggctggaac ggcccggtag aaatcgacca gatcaaaggt aagaaaatta ccgttgtgcc
    26461 aattctgcgt gcgggtcttg ggatgatgga cggtgtgctg gaaaacgttc cgagtgcgcg
    26521 catcagtgtc gtcggtatgt accgtaatga agaaacgctg gagccggtac cgtacttcca
    26581 gaaactggtt tctaacatcg atgagcgtat ggcgctgatc gttgacccaa tgctggcaac
    26641 cggtggttcc gttatcgcga ccatcgacct gctgaaaaaa gcgggctgca gcagcatcaa
    26701 agttctggtg ctggtcgctg cgccagaagg tatcgctgcg ctggaaaaag cgcacccgga
    26761 cgtcgaactg tatactgcat cgattgacca gggactgaac gagcacggat acattattcc
    26821 gggcctcggc gatgccggtg acaaaatctt tggtacgaaa taaagaataa aaataattaa
    26881 agccgacttt aagagtcggc ttttttttga gtaaagcgcc tataacacat aatacagagg
    26941 ataatactat gacgcgccgt gctatcgggg tgagtgaaag accgccactt ttacagacaa
    27001 tcccgcttag tttgcaacat ttgttcgcca tgtttggtgc aaccgtcctg gtgcccgtct
    27061 tatttcatat taacccggcg actgtactgt tatttaacgg tataggaaca ctgctgtatc
    27121 tcttcatctg taaagggaaa attccggctt atcttggttc cagctttgcc tttatttcac
    27181 cggtattgtt actgttgccg ttggggtatg aagttgcgct gggcggcttt attatgtgcg
    27241 gcgtgctgtt ctgcctggtt tcttttatcg tgaagaaagc ggggaccggc tggctggacg
    27301 tgctgtttcc acctgcggca atgggcgcaa tcgttgccgt catcggtctg gagctggcgg
    27361 gcgtagctgc cggtatggcg ggtttactcc cggctgaagg gcaaacgcca gactccaaaa
    27421 ccatcatcat ctctattacc accctggcgg tcacggtttt aggttccgtg ctgtttcgtg
    27481 gtttcctggc aattatcccg attttaattg gcgtgctggt ggggtacgcg ctctctttcg
    27541 caatgggaat tgtcgatacc acgccgatta ttaatgctca ctggtttgcg ctgccgaccc
    27601 tctatacgcc gcgcttcgag tggtttgcca ttctgactat tctaccagcg gcgttagtgg
    27661 ttattgccga acacgtaggg cacctggtcg taacggctaa tatcgtcaaa aaagatctgc
    27721 tgcgcgatcc aggtctgcac cgttcgatgt ttgctaacgg cttgtcgacc gtgatctccg
    27781 gcttctttgg ctccacgcca aacaccactt acggagaaaa cattggcgtg atggcgatca
    27841 cccgtgttta cagtacctgg gttatcggcg gtgcggcgat tttcgctatc ctgctttcct
    27901 gcgtcggtaa actggctgca gctatccaga tgattccatt gccggtgatg ggcggcgttt
    27961 cgctgctgct ttacggtgtc atcggtgctt ccggtattcg tgtgttgatc gaatcgaaag
    28021 tggattacaa caaagcacag aacctgatcc tgacttccgt tattttgatc atcggcgtca
    28081 gtggcgcgaa ggtaaacatt ggcgcggcgg agctgaaagg tatggcgctg gcgaccatcg
    28141 tcggtatcgg cttaagtttg atcttcaaac tgattagtat gctgcgccct gaagaagtgg
    28201 tgctggacgc agaagacgcg gatataacag acaaataatg taccgaccgg gcagtgttgc
    28261 tgcccggttc aaacatcatg ggattctgtg gtaaacttct cgcgattttg tgaaatcctg
    28321 gttgaggtat ctctgaacac accggcacag ctctctttgc cactttatct tcctgacgac
    28381 gaaacctttg caagtttctg gccgggggat aactcctctt tactggccgc gctgcaaaac
    28441 gtgctgcgtc aggaacatag cggttacatc tatctctggg cacgcgaagg cgcggggcgc
    28501 agccatctgc tgcacgcggc ttgcgcggaa ttgtcgcagc gtggcgatgc ggtgggctat
    28561 gtcccgctgg ataaacgcac ctggtttgtt ccggaagtgc tcgacggtat ggagcatttg
    28621 tcgctggtct gtatcgacaa cattgagtgt attgcaggcg atgagttgtg ggagatggcg
    28681 attttcgatc tctacaatcg aattctggaa tcgggcaaaa cacggttgtt aatcaccggc
    28741 gatcgtccac cgcggcagtt gaatcttgga ttaccggatc tcgcgtcgcg tctcgactgg
    28801 gggcagatct acaaattgca gccactttct gatgaagata agttgcaggc gctacagtta
    28861 cgcgcgcgtt tgcgtggttt tgaactgccg gaagatgtgg ggcgtttctt gctgaagcgg
    28921 ctcgacagag agatgcgcac gttatttatg acgttggatc agttggatcg tgcgtcgatt
    28981 accgcgcaac gtaagctgac cattccgttt gtgaaagaaa ttctgaaact gtagacaaga
    29041 cgtttattgt cggatatgca cccatagtat tcgtcggccg gataagacgc ggcaagcgtc
    29101 gcatccggca atgtgcttca actagcgatg gagcacttct taacccacga tttccagtac
    29161 ctgctccggt ggacgaccaa tccgcgcttt gccattcgcg accacaatcg ggcgctccat
    29221 tagcttcgga ttatccacca ttgcctgaat cagcgcctct tcgctaagcg aactgtcagc
    29281 caggttcagt tctttataaa gatcctcttt ctggcgcatc aattcacgag cgctattcat
    29341 ccccagcatt ttcagcaaat cacgcagcgt tgccgcatcg gcgggtgtct caaggtagag
    29401 caccacttct ggctccacgc cgttttcttt cagcaggttc agcgtttccc ggctctttga
    29461 acagcgtggg ttatggtaga tttttacctg tttggtcatt gttcttcctt taatgcgaat
    29521 tacatcttgg tataaggctt aaagcgttcc tgcagctggc gcaactggtc gatgcgcgca
    29581 tcgtaacgcg cttgttgcag gctgcctaat ttcacctgcg aactggcgct actcaacagc
    29641 gaaatggcct gatcgagtcg tccggcgagc gcataacctt ctgctcgcgc agccagctcc
    29701 tgatcgcggt tatttagcgc ggcttccgcc tgtgccagca aatcccagcc gttgctgtca
    29761 tctttattat taaaggtgta gcgattcaga atattggccg cttcttgtgg ttgaccgcct
    29821 tgcagataag cgttcgccag gttgagctgc aacaccggat tggtgcgtaa atcgcgggca
    29881 tttttcagac gattgatcgc ctcattggct ttgttttgcc caagatcgat atcagtagcc
    29941 agatcgagat accatgcgtt gccaggttct gccgccagta acggttgcag cgttttacgc
    30001 gcctcgtcgt atttattggc ttccatcgcc tgtaaagcac gaccatattg cgccgctcgt
    30061 tgctgacgaa cgtttccttt cgcccattca tccagcaaat cactggtgag ctggttacgt
    30121 ccggaattat acatccccag tgtgcgcgct ttcgccagat agaaatcttc cgacgactgc
    30181 accaccatcg ggcgcatctg attagcacgg ttgcgggcat ctgccagacg actttccggc
    30241 aacgggtgag tcaataaaat ttccggcgga cgcgaggagt aacgcgcctg atcgagtaat
    30301 ttttcgagga aggtcggcat cgcctgcgga tcgaatcctg agcgttgcag cacctgaata
    30361 ccaatgcggt ccgcttcctg ttcattttgc tgggtgaagc tgatcatccc ctggcgcgtt
    30421 cccgccagtg taccggtcag cgcagccatc cccgcctgcg gactggccat cgccagtaaa
    30481 atagaaccta acgcgccgac ccaggtcagc ggcgcgctgc gctgctgatc ttccatcgct
    30541 cgcgccaggt gacgttgggt gacgtgggag atttcgtgcg ccataactga agccagttga
    30601 ctttcgttat cggaataacg gaacagggca gagtgcagca ccacgttgcc gccaaagaaa
    30661 gcaaaggcgt taatttcatc gttgttgatc agaaagaaat gaaacggtgt cttaaccgaa
    30721 ttggcatgcg aaaccagacg cattcctagc gaattaatgt attgcgttaa cagcggatca
    30781 ttaattaacg gcgcgctgcc gcgtagctgg cggacataat agtcgcccat ctgcatttcc
    30841 tgaccaatgg aaagtgtgct tcctgcggag gttcccatat ccggcaaggt gtctgcgctg
    30901 tcggcaaacg ccggggctac ctgaccaatg gtcatagcag caatgagggt tgcaaccagg
    30961 ttttttttca actgcctgaa cataacctct atcctgtatt tctggagata gtcatttgac
    31021 cgatactgtg aaatatcgtt cccaccgcgc cgcctttgag tgtaacggcg aagattagcc
    31081 cactcaaatg acttttcagt ttcctgaatc cgtcataaaa agcgaagtct tttttgtggc
    31141 atttcgatac aattgcggat cgcaatcccg ctcttgaggt tcagggaagg tctgtatgct
    31201 cgaaatgttg atgcaatggt atcgccgccg ttttagcgac ccggaagcga ttgccttgct
    31261 ggttatttta gttgccggat ttggcattat ctttttcttt agtggcctgc ttgctccgct
    31321 gttggtggct attgtgctgg cctatttgct ggaatggcca accgtgcgcc tgcaatctat
    31381 tggctgctcc cgccgctggg cgacgtcgat tgtattggtg gttttcgtcg gtatattgct
    31441 actgatggcg ttcgtggtac tgcctatcgc ctggcagcag ggcatctact taatccgcga
    31501 tatgccgggg atgctcaata agctttctga ctttgccgcc acgttgccgc gccgctatcc
    31561 ggcgttaatg gatgcgggca ttattgatgc aatggccgaa aatatgcgca gtcggatgct
    31621 gaccatgggc gattcggtgg tgaaaatttc cctcgcctcg ctggtcggtt tgctgaccat
    31681 tgccgtctat ctggtgctgg taccattgat ggtcttcttc ctgctgaaag acaaagagca
    31741 gatgctgaac gccgttcgcc gggtgctgcc gcgtaaccgt ggactggcag gacaggtgtg
    31801 gaaggagatg aatcaacaaa tcaccaacta tatccgcggc aaagtgctgg agatgatcgt
    31861 ggtggggatt gccacctggc tgggattctt actcttcggg ctgaactatt cgctgctgct
    31921 ggcggtgctg gtcggcttct cggtacttat tccgtacatt ggtgcatttg tggtgaccat
    31981 tccggtggtt ggcgtggcgc tattccagtt tggtgctggc acggaattct ggagctgctt
    32041 cgcggtgtat ctgatcattc aggcgctgga cggcaacctg ttagtacctg tgttgttctc
    32101 cgaagcggtt aacctgcatc cgctggtcat tattttatca gtagtgatct tcggtggttt
    32161 gtggggattc tggggcgtat tcttcgccat tccattggcg acgctgatca aagccgtgat
    32221 tcacgcctgg cccgatgggc aaatcgcgca agactgacct tcagcgaaaa aatttgaaac
    32281 gagtgttgca gggcagtgag aagatgtgag atgtgctggc cgcaagcgca gccagcacgg
    32341 aatggagcaa agtaatcagg cgtgttcttt cagccagttc agcacaacgt cgtggtgatt
    32401 gctggttttg aaatcgtcaa agacatgttc gattttgcca tcagcgtcaa tcaggaagct
    32461 gatgcgatga ataccatcgt aggttttgcc catgaaggac ttttcacccc agacgccgaa
    32521 ttgttcgcac acctggtggt cctcatcaga caggagcgta aagttaagca gctctttttc
    32581 cgcaaaacgg gagagttttt cgggtttatc ggtgctgata cccagcacat caacgcccgc
    32641 ttttttcaac tcatccatgt tatcgcgtaa gccgcaggcc tgtacggtac agccgggggt
    32701 catggctttc gggtagaaat aaaccagaac acgctgtccc tggaagtcgg tcaaattaac
    32761 ttgttctccg tcttgatccg gcaagctaaa tttcggtgcg atatcaccgg ctttcagtgg
    32821 attcattact taactccatc ctgttcatca tgttgggaat aattgacgac gttaatactg
    32881 ccttgtgcat tgagttctgt acatagggct ttgaatgctt gctcaatatt tgctgcgtcc
    32941 gcagatgcgg ggctgtgggc ggttatctga atatgcaact gcgcagcccg ttcattttca
    33001 gcaggttgcg tgcgcgacac cagctccgca atgttcatat gatgcgcgtc gaaaagtgct
    33061 gtgaagcgtt caattaaatg cggggagtct gccacatcga cctgaaccca gacagatgct
    33121 ggcattggcg gacgcggacg cgccgtcgtg cgcttcatca cgattaaaag atccagctcg
    33181 gcacctttca acggtaacgt tgattcaatc agagtaatgg cattccatga accggaaagc
    33241 agcatgataa acgtgaactc ttctcccagc atcgccaggc gactgtcttc aatattacag
    33301 ccgcaactac tgacatgacg ggtgatggtg ttcacaattc cagggcgatc ggcacccaac
    33361 gcagtgatca ccagataatg ttgcgatgac agtgtcaaac tggttattcc ttcaaggggt
    33421 gagttgttct taaggaaagc ataaaaaaaa catgcataca acaatcagaa cggttctgtc
    33481 tgcttgcttt taatgccata ccaaacgtac cattgagaca cttgtttgca cagaggatgg
    33541 cccatgttca cgggaagtat tgtcgcgatt gttactccga tggatgaaaa aggtaatgtc
    33601 tgtcgggcta gcatgaaaaa actgattgat tatcatgtcg ccagcggtac ttcggcgatc
    33661 gtttctgttg gcaccactgg cgagtccgct accttaaatc atgacgaaca tgctgatgtg
    33721 gtgatgatga cgctggagct ggctgacggg cgcattccgg tgattgccgg gaccggtgct
    33781 aacgctactg cggaagccat tagcctgacg cagcgcttca atgacagcgg tatcgtcggc
    33841 tgcctgacgg taacccctta ctacaatcgt ccgtcgcaag aaggtttgta tcagcatttc
    33901 aaagccatcg ctgaacatac tgacctgccg caaattctgt ataatgtgcc gtcccgtact
    33961 ggctgcgatc tgctcccgga aacggtgggc cgtctggcga aagtaaaaaa tattatcgga
    34021 atcaaagagg caacagggaa cttaacgcgt gtaaaccaga tcaaagagct ggtttcagat
    34081 gattttgttc tgctgagcgg cgatgatgcg agcgcgctgg acttcatgca attaggcggt
    34141 catggggtta tttccgttac ggctaacgtc gcagcgcgtg atatggccca gatgtgcaaa
    34201 ctggcagcag aagggcattt tgccgaggca cgcgttatta atcagcgtct gatgccatta
    34261 cacaacaaac tatttgtcga acccaatcca atcccggtga aatgggcatg taaggaactg
    34321 ggtcttgtgg cgaccgatac gctgcgcctg ccaatgacac caatcaccga cagtggtcgt
    34381 gagacggtca gagcggcgct taagcatgcc ggtttgctgt aaagtttagg gagatttgat
    34441 ggcttactct gttcaaaagt cgcgcctggc aaaggttgcg ggtgtttcgc ttgttttatt
    34501 actcgctgcc tgtagttctg actcacgcta taagcgtcag gtcagtggtg atgaagccta
    34561 cctggaagcg gcaccgcttg cggagcttca tgccccggct ggaatgattt tgccggtgac
    34621 ctccggcgat tatgcaatcc cggtgaccaa cggtagtggt gctgtcggta aggcgctgga
    34681 cattcgtcca ccagcccagc cgttggcact ggtttctggc gcgcgtaccc agttcacggg
    34741 cgataccgct tcattgctgg tggaaaatgg tcgtggcaat actctgtggc cgcaggtggt
    34801 tagcgtgctg caggcgaaaa actacaccat cacccaacgt gatgatgctg gtcagacact
    34861 gaccaccgat tgggtacaat ggaaccgtct ggacgaagac gagcagtatc gtggtcgtta
    34921 tcaaatctct gttaagccgc agggttatca gcaggcggtt acggttaaac tgctgaacct
    34981 ggaacaggcg ggcaaaccgg ttgcagacgc ggcttccatg cagcgttaca gcacggagat
    35041 gatgaacgtt atttccgccg gtctggataa atctgccact gacgccgcga acgctgcgca
    35101 aaatcgtgcc tccaccacta tggacgtaca aagtgcagct gatgacaccg gtttaccaat
    35161 gctggtcgta cgcgggccgt tcaatgtggt ttggcaacgt ctgccagctg cgctggaaaa
    35221 agtgggcatg aaagtgaccg acagcacccg ttcgcagggc aacatggccg taacttacaa
    35281 gccgctgtct gacagcgaat ggcacgaact gggcgcgagc gatccaggtc tggcatccgg
    35341 tgactataaa ctgcaggttg gcgatttaga taaccgcagc agcctacagt tcatcgatcc
    35401 gaaaggtcat actctgactc agagtcagaa cgacgcgctg gtagctgtct tccaggctgc
    35461 gtttagcaag taaaaataca gggctggagt catccggccc ttttttctga tatgatacgc
    35521 aaacgtgtgc gtcggcagaa aaacgcgatt ttagcggtaa ttcgcacgaa atttgtttgt
    35581 cggacgtagg tcggataagg cgttcacgcc gcatccgaca aaacatccgg cacaccagac
    35641 agcaatagat tttaaaacgt taattcacac ccaggagtga taaagatgca aaagcaagct
    35701 gagttgtatc gtggtaaagc gaaaaccgta tacagcacgg aaaacccgga cctgttggtg
    35761 ctcgaattcc gcaatgatac gtcagcaggg gatggcgcgc gcattgagca gtttgatcgc
    35821 aaaggtatgg tgaacaacaa gttcaactac ttcattatga gcaaactggc tgaagcgggt
    35881 atcccgactc aaatggagcg tctgctctcc gataccgaat gtctggtgaa aaagctggat
    35941 atggtgccgg ttgagtgtgt cgtgcgtaac cgtgctgctg gctctctggt gaaacgtctt
    36001 ggaatcgaag aaggtattga gctgaacccg ccactgttcg atctgttcct gaaaaacgac
    36061 gccatgcacg atccgatggt caacgaatct tactgcgaaa cctttggctg ggtaagcaaa
    36121 gagaacctgg cacgcatgaa agagctgacc tacaaagcga acgacgtgct gaaaaaactg
    36181 ttcgatgatg ctggtctcat tctggtcgac ttcaagctgg agtttggtct gtacaaaggt
    36241 gaagtggtac tgggcgatga gttctccccg gacggcagcc gcttgtggga caaagaaacg
    36301 ctggagaaaa tggacaaaga ccgtttccgc cagagcctcg gtggcctgat cgaggcctat
    36361 gaagccgtcg cccgccgcct gggtgtacag ctggactgat ttttctgttc atcatcttgc
    36421 cgtgcagctg gcacggcaag acaaccgctc tcataagatg agtattgaac gctcattcct
    36481 ccgccatttc atcccgcttt cctgtggtaa tcttcctctg aaccacctac gatcatttct
    36541 ataatgaata tattgttgag gtatctatgc gttggcaagg gcgacgtgaa agtgacaatg
    36601 ttgaagacag gcgcaacagc tctggtggtc cgtcaatggg cggtccaggt tttcgcctgc
    36661 caagcggtaa aggcgggctg attttactga tagtcgtgct ggttgcaggc tactatggcg
    36721 ttgatttaac cgggttgatg accggacagc cggtttccca acagcaatca acacggtcaa
    36781 ttagtcctaa tgaagacgaa gccgcaaaat tcacttcggt gattctggca accacggaag
    36841 acacctgggg acagcaattc gagaagatgg gtaaaaccta tcagcaaccg aagctggtca
    36901 tgtaccgtgg aatgacgcgc accggctgcg gggcgggcca gtccataatg ggaccgttct
    36961 attgcccggc ggatggcacg gtttatatcg atctctcctt ctatgatgac atgaaagaca
    37021 aacttggcgc ggatggcgat tttgcccagg ggtacgttat cgcccatgaa gtcggtcatc
    37081 atgtgcagaa actgttaggc atcgagccga aagttcgtca actgcaacaa aacgcgacgc
    37141 aggcggaagt gaaccgctta tctgtgcgta tggaactcca ggccgactgt tttgccggtg
    37201 tctgggggca tagtatgcag cagcaaggtg ttctggaaac cggcgatctg gaagaggcgc
    37261 tgaacgcggc gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag
    37321 taccagacag tttcactcat ggcacttctc agcaacgcta cagctggttt aaacgtggtt
    37381 tcgacagcgg cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc
    37441 agggatggct gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat
    37501 ccgccgcttg ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt
    37561 gcgtgatgcc ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca
    37621 ctgttctccc tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga
    37681 cgcccgccac ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg
    37741 aagctggctg gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga
    37801 ctcgctgcga tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct
    37861 caaacgcgta cttacggcga ataacgacgc tatcctctgg cggcagaacc agccgttcac
    37921 gttggcgcat tttactcccc gtactgactg gcaccccgcg accggcgcac cacagccaga
    37981 acaacagcaa ctcttacagc agctactgac catgccattg ggcgtggcgg tggtaacggc
    38041 tgcgcgtggg cgcggtaaat cggcgctggc agggcaactc atttctcgta ttgcgggtag
    38101 tgcgattgtc accgcgcccg caaaagcggc aacgtatgta ctggcacaat ttgcgggcga
    38161 gaagtttcgc tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct
    38221 ggtggtcgat gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt
    38281 tcctcgaacg ttgttaacca ctacggtgca gggctacgaa ggcacgggac gtggtttttt
    38341 gctgaaattt tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg
    38401 ctgggcacag ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttgacgatga
    38461 aaacttcacc cacacaccac aaggcaatat cgtcatttcc gcatttgaac agacgttatg
    38521 gcgaagcgag ccacaaacgc cgttaaaggt ttatcaatta ttgtctggtg cgcactaccg
    38581 gacttcgccg ctggatttac gccgcatgat ggatgcacca gggcaacatt ttttacaggc
    38641 ggctggcgaa aacgcgattg ccggggcgct gtggctggtg gatgagggcg gattatctca
    38701 agaactcagt caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca
    38761 gtcgctggcg gcgcacggca gcaatccact ggcggcgaca ttgcgcggaa ggcgggttag
    38821 ccggatagca gttcatccgg ctcgtcagcg ggaaggcaca ggtcagcaac tgattgctgg
    38881 tgctttgcaa tatacgcatg acctcgacta tctttcggtg agttttggtt acaccgggga
    38941 attatggcgt ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga
    39001 agccagcagc ggttgctata cggcgatggc gctgttaccg atgagtgatg cgggtaaaca
    39061 gctggctgaa cgtgagcatt accgtttacg tcgcgatgcg caagctctcg caaagtggaa
    39121 tggcgaaacg ctccctgttg atccactaaa cgatatcgtg ctttctgccg acgactggct
    39181 tgaactggcc ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttaat
    39241 gcgtctgtta caaaccagcg aaatggcatt accggcgctg cgtgggcgtt tacagaaaaa
    39301 cgccagcgac gcgcagttat gtaccacact taaactttca ggccgtaagc tgttactggt
    39361 ccgtcagcgg gaagaggccg cgcaggcgct atacgcactt gatgatgttc gcactgagcg
    39421 tttgcgcgat cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat
    39481 ggtcattgtg agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac
    39541 atgaccattt tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc
    39601 acggtgtcgg ggataacccg gtggcaatgg gggaaatagg tagctggttt gcgccgctat
    39661 ttcctgatgc gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc
    39721 gtcagtggtt ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca
    39781 tcatgccgac gtttattgag acggtgcgct actggcagaa acagagcgga gtaggggcta
    39841 ccgccacggc gttaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag
    39901 ccgaaccggg ccttgcctca cgcgtcattg cctttaacgg acgctatgcg agcctgccgg
    39961 aaactgcgtc tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg
    40021 atctggcgca tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc
    40081 tggatatcgt ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgctctgg
    40141 atcacttgcg ttataccatt ccgaaacatt atttcgacga agcgctaagc ggcggtaagc
    40201 ctggcgatga tgatgtgatt gaaatgatgt aataatagcc gggtaggtct gataagactg
    40261 tatcaggcat catggccgga tgcggcgtta acgccttatc cggccactta gcttacttct
    40321 tcggttgatc ctttttcggc cagtcgtcgt catcgtccca cttatcgttg aaatcacgat
    40381 gcgggggaag ctccggcttg ttggcgagaa atttcttatg gttgacgcgc ttgagatctt
    40441 taatcacgtt cagcaggacg cctaccaaaa agacaatcac cagaatccac caatatttag
    40501 ccagccagtc catgcttatt tcctcttgca gaaccactca tcaggcgacg agctgttcca
    40561 tgatacgttg atacatacgg gcaagtagct gcaggtcggc agcgttcaca cattcattaa
    40621 ttttatgaat agtggcattg accggcccga gttccaccac ctgcgccccc atgcgggcaa
    40681 taaagcgccc gtcggacgtt ccgcctgtgg ttagtagctg cggtttaatt tcattatagt
    40741 gctcaaccgc gttaacgacc gcatccacca gtttaccgcg cgcggtcaaa aatggctgcc
    40801 cggaaagcca ccaatccacc gtatagcgca gttgatgttt ttcaagcagg gcaagtacct
    40861 gcgctttgat catctcatca gtcagttcgg tgctgaagcg gaagttaaac tgcacaaaca
    40921 gttcacccgg aataacgttg ttactgcccg ttcccgcctg aatattggca atctgcatac
    40981 tggtcgccgg gaagaattca ttgccctgat cccactcaat agccactaat tcattaagga
    41041 aaggtgctgc gcgatgtacc ggattgtcag ccagatgtgg gtaggcaaca tgcccctgca
    41101 cgccatgaat ggtgaggttg caggttaacg atccgcgacg accatttttc accacatcac
    41161 caaccacttc gatactcgac ggttcgccaa ccagacagta atcgagacgc tcattacgcg
    41221 ccattaacgc ttcgacgact tttaccgtac cgttgtgggc actagcttct tcatcagagg
    41281 tgatcagaaa tgccagtcgc cccgtatggt tgggatgttg tgcgacaaaa cgttctgccg
    41341 ctaccaccat cgccgccagc gagcctttca tatctgccgc accgcgcccg aataacatgc
    41401 cgtcacgaat ggtgggttca aacggcggat tgatccaacg gtcggcgtcg ccaggcggca
    41461 ccacgtcggt atgcccggca aaggctaacg tttcaccctg cccacgccat gcccaaaaat
    41521 tctgcgtatc ggcaaagtcc atgcgttcaa cggtaaaacc gatcgcctgc aaacgttcaa
    41581 tcaacaaagc ctggcatcct gcgtcatcag gactcaggga agggcggcga ataagctgtt
    41641 gtgtcagctc aataaccggg cacgacatag actacacctc atggaaaaat tgctgataac
    41701 tggattcact gaaacccagc agcataggct taccaggcgc gcagagcaat ggacgtttga
    41761 taattgcagg catttcagtc attaatgtcg ccgcagaggc cgcatcggtg attttattgc
    41821 gggtggtttc gtccagttta cgccaggttg taccacgggt gttgagtaac gcttcccagc
    41881 ctaattcgtt gataaaaccg ttcaataatt cgctgtccaa tccatcgacg cggtaatcat
    41941 gaaagcgata gtcgatgtta ttggcttcca gccaacggcg agccttttta atggtgtcac
    42001 aatttttgat gccgtaaagt gtaaccataa tgaatccttt ttcg
//
LOCUS       DAZGHF010000024        41599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGHF010000024 DAZGHF010000000
VERSION     DAZGHF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.024"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(198..464)
                     /locus_tag="ACHK3A_001436"
                     /pseudo
     CDS             complement(198..464)
                     /locus_tag="ACHK3A_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(872..2077)
                     /locus_tag="ACHK3A_001437"
     CDS             complement(872..2077)
                     /locus_tag="ACHK3A_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8739544.1"
                     /translation="MALTDIKVKTAKPKEKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGVAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFKKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLACALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFKTKLWEIPAEVMKMKRPHIVP
                     LSEQVIALFKQLEPITKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIETQLAHVDKNAIRGTYNHALYLDGRREMMQWYADYIDSLSSH
                     ESQCQGPLY"
     gene            complement(2377..2471)
                     /locus_tag="ACHK3A_001438"
     tRNA            complement(2377..2471)
                     /locus_tag="ACHK3A_001438"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2435..2437),aa:Sec,seq:tca)
     gene            2764..4146
                     /gene="yicJ"
                     /locus_tag="ACHK3A_001439"
     CDS             2764..4146
                     /gene="yicJ"
                     /locus_tag="ACHK3A_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO8739545.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            4156..6474
                     /gene="yicI"
                     /locus_tag="ACHK3A_001440"
     CDS             4156..6474
                     /gene="yicI"
                     /locus_tag="ACHK3A_001440"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO8739546.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKHPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAVCEMPAADGSVIFTLKATRTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLMVTPQGNALTITL"
     gene            6770..8323
                     /locus_tag="ACHK3A_001441"
     CDS             6770..8323
                     /locus_tag="ACHK3A_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFO8739547.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENITDLLHLDHLLVAECEEIDGVMNNSRRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            8350..8820
                     /locus_tag="ACHK3A_001442"
     CDS             8350..8820
                     /locus_tag="ACHK3A_001442"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFO8739548.1"
                     /translation="MDITKILNTNRVILDMQATNKEEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            8838..9152
                     /locus_tag="ACHK3A_001443"
     CDS             8838..9152
                     /locus_tag="ACHK3A_001443"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO8739549.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            9177..10268
                     /locus_tag="ACHK3A_001444"
     CDS             9177..10268
                     /locus_tag="ACHK3A_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO8739550.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            10280..11131
                     /locus_tag="ACHK3A_001445"
     CDS             10280..11131
                     /locus_tag="ACHK3A_001445"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO8739551.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASVQLCVEGELGHLADAPRYDQAANADLMTQPTDVET
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            11212..12072
                     /locus_tag="ACHK3A_001446"
     CDS             11212..12072
                     /locus_tag="ACHK3A_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO8739552.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPT
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            12120..13052
                     /locus_tag="ACHK3A_001447"
     CDS             12120..13052
                     /locus_tag="ACHK3A_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO8739553.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            13033..13713
                     /locus_tag="ACHK3A_001448"
     CDS             13033..13713
                     /locus_tag="ACHK3A_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO8739554.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(13757..15466)
                     /gene="yicH"
                     /locus_tag="ACHK3A_001449"
     CDS             complement(13757..15466)
                     /gene="yicH"
                     /locus_tag="ACHK3A_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO8739555.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGSWLRDGKTLLLDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVTGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(15587..16978)
                     /gene="xanP"
                     /locus_tag="ACHK3A_001450"
     CDS             complement(15587..16978)
                     /gene="xanP"
                     /locus_tag="ACHK3A_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO8739556.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            17257..18462
                     /gene="gltS"
                     /locus_tag="ACHK3A_001451"
     CDS             17257..18462
                     /gene="gltS"
                     /locus_tag="ACHK3A_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO8739557.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(18496..20577)
                     /gene="recG"
                     /locus_tag="ACHK3A_001452"
     CDS             complement(18496..20577)
                     /gene="recG"
                     /locus_tag="ACHK3A_001452"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO8739558.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(20583..21272)
                     /gene="trmH"
                     /locus_tag="ACHK3A_001453"
     CDS             complement(20583..21272)
                     /gene="trmH"
                     /locus_tag="ACHK3A_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO8739559.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(21279..23387)
                     /gene="spoT"
                     /locus_tag="ACHK3A_001454"
     CDS             complement(21279..23387)
                     /gene="spoT"
                     /locus_tag="ACHK3A_001454"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO8739560.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(23406..23681)
                     /gene="rpoZ"
                     /locus_tag="ACHK3A_001455"
     CDS             complement(23406..23681)
                     /gene="rpoZ"
                     /locus_tag="ACHK3A_001455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO8739561.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(23736..24359)
                     /gene="gmk"
                     /locus_tag="ACHK3A_001456"
     CDS             complement(23736..24359)
                     /gene="gmk"
                     /locus_tag="ACHK3A_001456"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO8739562.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            24617..26302
                     /gene="ligB"
                     /locus_tag="ACHK3A_001457"
     CDS             24617..26302
                     /gene="ligB"
                     /locus_tag="ACHK3A_001457"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO8739563.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGEETHRDAMMPPLNGAVIHPVAHTGVR
                     KMADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVR
                     LISAVPQTVSGPLANSTLQGEIFLKREGHIQKQMGGINARAKVAGLMMRQDDSDTLNS
                     LGVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVARVRNAWWKTKLPFV
                     TDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVARVAEVKAIQFAVGKSGKISVVASL
                     APVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPT
                     PPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRSLHQTHRFEHIF
                     SWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDER
                     SWSQLLLSTEQFWQQLPGTGSGRVRQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(26299..26916)
                     /gene="yicG"
                     /locus_tag="ACHK3A_001458"
     CDS             complement(26299..26916)
                     /gene="yicG"
                     /locus_tag="ACHK3A_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO8739564.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(27208..28032)
                     /gene="dinD"
                     /locus_tag="ACHK3A_001459"
     CDS             complement(27208..28032)
                     /gene="dinD"
                     /locus_tag="ACHK3A_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO8739565.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVNIKQLENSVKITEKK"
     gene            complement(28254..29117)
                     /gene="yicC"
                     /locus_tag="ACHK3A_001460"
     CDS             complement(28254..29117)
                     /gene="yicC"
                     /locus_tag="ACHK3A_001460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO8739566.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            29244..29960
                     /gene="rph"
                     /locus_tag="ACHK3A_001461"
     CDS             29244..29960
                     /gene="rph"
                     /locus_tag="ACHK3A_001461"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO8739567.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            30026..30667
                     /gene="pyrE"
                     /locus_tag="ACHK3A_001462"
     CDS             30026..30667
                     /gene="pyrE"
                     /locus_tag="ACHK3A_001462"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO8739568.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(30704..31300)
                     /gene="slmA"
                     /locus_tag="ACHK3A_001463"
     CDS             complement(30704..31300)
                     /gene="slmA"
                     /locus_tag="ACHK3A_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO8739569.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(31407..31865)
                     /gene="dut"
                     /locus_tag="ACHK3A_001464"
     CDS             complement(31407..31865)
                     /gene="dut"
                     /locus_tag="ACHK3A_001464"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO8739570.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(31843..33063)
                     /gene="coaBC"
                     /locus_tag="ACHK3A_001465"
     CDS             complement(31843..33063)
                     /gene="coaBC"
                     /locus_tag="ACHK3A_001465"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO8739571.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRERGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            33235..33903
                     /gene="radC"
                     /locus_tag="ACHK3A_001466"
     CDS             33235..33903
                     /gene="radC"
                     /locus_tag="ACHK3A_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO8739572.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            34120..34356
                     /gene="rpmB"
                     /locus_tag="ACHK3A_001467"
     CDS             34120..34356
                     /gene="rpmB"
                     /locus_tag="ACHK3A_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO8739573.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            34377..34544
                     /gene="rpmG"
                     /locus_tag="ACHK3A_001468"
     CDS             34377..34544
                     /gene="rpmG"
                     /locus_tag="ACHK3A_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO8739574.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            34642..35451
                     /gene="mutM"
                     /locus_tag="ACHK3A_001469"
     CDS             34642..35451
                     /gene="mutM"
                     /locus_tag="ACHK3A_001469"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO8739575.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGESCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(35490..35969)
                     /gene="coaD"
                     /locus_tag="ACHK3A_001470"
     CDS             complement(35490..35969)
                     /gene="coaD"
                     /locus_tag="ACHK3A_001470"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO8739576.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(35977..37254)
                     /gene="waaA"
                     /locus_tag="ACHK3A_001471"
     CDS             complement(35977..37254)
                     /gene="waaA"
                     /locus_tag="ACHK3A_001471"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO8739577.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            37667..38725
                     /gene="rfaQ"
                     /locus_tag="ACHK3A_001472"
     CDS             37667..38725
                     /gene="rfaQ"
                     /locus_tag="ACHK3A_001472"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO8739578.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            38722..39846
                     /gene="waaG"
                     /locus_tag="ACHK3A_001473"
     CDS             38722..39846
                     /gene="waaG"
                     /locus_tag="ACHK3A_001473"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO8739579.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQFSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            39839..40636
                     /gene="rfaP"
                     /locus_tag="ACHK3A_001474"
     CDS             39839..40636
                     /gene="rfaP"
                     /locus_tag="ACHK3A_001474"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO8739580.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKKEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVASLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            40652..>41599
                     /gene="waaO"
                     /locus_tag="ACHK3A_001475"
                     /gene_synonym="rfaI"
     CDS             40652..>41599
                     /gene="waaO"
                     /locus_tag="ACHK3A_001475"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFO8739581.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKLIEAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNF"
BASE COUNT        10079 a        10002 c        10932 g        10586 t
ORIGIN      
        1 gtaaaagtcc ctattataga agccgtagca aaacgaaatt ttaacagaag caacttcatt
       61 ttgtaagtgt ccggcttaat aatgaagagc tcacgcttct aaacgataag cgtggtagcc
      121 atcggcttcc tttatttttt cttaatgtgc ttcctagtat tgttccagtc attaatatag
      181 aagcatggga tctttagcag gcttttcaca gaaataaata aaaatattgc ccaccttggc
      241 tgctccagaa atgcatatgc cagatgagcg tgtatatcaa taaagtcagg ctcccgttgg
      301 gcaagctacc tgagtttgtt gatataactc gtctcgctga tttcatcagt gccgtatgcg
      361 tcatgcactt cttcaaactc ctccagtaat gggccaaacc tggtattcgg aaatacgaaa
      421 cggccttggt tatcgttcac atcaaagcgt aatctgtcgt tcattttctg agcctgtaaa
      481 acggatggga cacaatctga aaagcataaa agtcgtgatg ttgtgtatca agtcttgtta
      541 ttgcccggga tgaatattgt ttacaatatc atcgacagta attcttcatg tggataaaaa
      601 tttatcttta tctgcattcc aatgcattgt gaatagtcgg ctaagacaac atgttagttg
      661 atttttggtg tgatggtgct gaaataacga caggatgatg attgaaatta caggcatcaa
      721 aattgttagc ggtctatagc ggtgtgatgt tctgagattg tttgcagatg caatataaat
      781 attttgtcaa aaaatatcta aaaataaaac atgaaaacaa aattcctcca gacctggagg
      841 ccggaggaat tttgtgaatt gagagaaaat atcagtacaa ggggccttga cactgactct
      901 cgtggctgga aagcgaatca atgtaatcag cgtaccattg catcatttca cggcgaccat
      961 ctagatacag ggcatgatta taggtccccc tgatagcgtt tttatctaca tgagctaact
     1021 gcgtttcaat ccaagcggaa ttaaagccct gttcgtgtag aatggtactc atggtgtgtc
     1081 tgaaaccgtg ccctgtgagt cttcccttat atcccagtaa ttcaatgact tggttaatgc
     1141 tttccttact aattggcttg cgaggatcgt tcctaccgat aaagaccaga ggatgatgtt
     1201 ttgtgatggg ttcaagttgc ttaaataggg caatgacctg ctcagagagc ggcacaatat
     1261 gaggtcgctt catcttcatt acttctgccg gaatctccca taattttgtc ttaaaatcga
     1321 tatcctccca gcgtgcaaaa cgtaattctt gtgttcgtac accggttaac ataatgatct
     1381 gagtagctgt tttagtgatg atacttccgg tataacccgc cagatcgttg aggaagtggg
     1441 gaagttcatt agcagtaaga aagggaaaat gtactttttt cggtgtagct aaagcacagg
     1501 caagatcggg agcagggttg tagtctgccc gaccagtaac aattgcatag cggaacacct
     1561 caccacagcg ctggcggact ttgcgcattt tctccagagc tccacgtttt tccattttac
     1621 gcagtgcttc cagtaactcc attggtttga tttcggcgat agggcgtttg ccaatgtagg
     1681 gaaaaatgtc ttttttaaag gtatcaatga tttcatcacg atagcgtaat gaccaccgat
     1741 cggcttttga ctgatgccat tcgcgagcaa tggcctcgaa cgtatttttc aaactcattt
     1801 gctgcgcgat tttttcttct ttttttgcca cccctggatc gcctccagca gcgaggattt
     1861 ttctggcttc gctgcgtttt tcacgagcgt cggctagcgt tacatcgggg tatacgccaa
     1921 tagaaagcat cttttcttta ccagcaaagc ggtacttcat acgccagtat ttagaaccat
     1981 tagtgttaat cagtagatac ataccgccgc catcagcaag tttatagggc ttttcttttg
     2041 gttttgcggt tttcacttta atatcggtta atgccatgac atcctccggc ttttaatgaa
     2101 ttcatgttcc tgagggggat ttctgccagt aattaacctg atacccccaa tgcacccctt
     2161 gtataacaaa gatgttgttg aaagccgttg ttatatatca tattttacca attatggggg
     2221 tgctgatatt tcaataggaa ataatacccc ctaaagtacc cccaaaagca acttgatacg
     2281 gggtgaagtc ggttgacttc tggatacaaa ggaatcctga tatacaaggg attagaaggg
     2341 ggggattgac atcgggagag ttcagaagat gttaattggc ggaagatcac aggagtcgaa
     2401 cctgcccggg accgctggcg gccccaactg gatttgaagt ccagccgcct caccggagac
     2461 gacgatcttc cgcgcctcga ttgctacatg gaggcggggc gcattatagc tacttccttg
     2521 agtttctaca tcccccagat cagttttttc cgcctaatac acgcagcgta ttatcacgaa
     2581 aacattatag ttcatataaa actcatattt tacaatgata tatgagcgaa attaacgcta
     2641 ccacgatcac ataaaacaag aatcataaat taataaccag atatcggaat attcgctctc
     2701 gcagggatgg tttacaaaat gcgttatcgg tctcagcacc cctatagcat caaggaaaag
     2761 cagatgaaga gtgaagtgtt gtccgttaaa gagaaaattg gttatggcat gggagacgcc
     2821 gccagccaca ttattttcga taacgtaatg ttatatatga tgttctttta taccgatatt
     2881 tttggcattc ctgccggatt tgtcggaacc atgtttttgg tcgctcgtgc actggatgcg
     2941 atttccgatc cttgcatggg gttgttggcc gatcgaacgc gctctcgctg gggtaaattt
     3001 cgtccgtggg tactgtttgg cgcactgcca tttgggatcg tctgtgtact ggcctatagc
     3061 acgccagatc tcagtatgaa cggaaaaatg atttatgcag caattactta caccctactt
     3121 accttacttt ataccatcgt taatatccct tactgcgcat tgggtggtgt aatcaccaat
     3181 gacccgactc agcgtatctc gctgcaatcc tggcgttttg tgctggcgac cgcgggaggc
     3241 atgctctcta ctgtgctgat gatgccgctg gttaatttaa ttggtggtga taataaacca
     3301 ctcggtttcc agggcggtat cgcggtcctt tccgtggtgg cattcatgat gctggcattc
     3361 tgtttcttca ccactaaaga acgcgttgaa gcaccaccta caacgacgtc tatgcgggaa
     3421 gatttacgtg atatctggca aaacgaccag tggcggattg tcggtttact aaccattttc
     3481 aatattctgg cggtgtgcgt acgcggtgga gcgatgatgt attacgtcac atggattttg
     3541 ggtacgccgg aagtgtttgt cgcttttctc accacttatt gcgtgggtaa cctgattggt
     3601 tccgcactgg caaaaccgct gaccgactgg aaatgtaaag tcactatctt ctggtggacg
     3661 aacgccctgc tggcagtgat tagcctcgcg atgttctttg ttcccatgca ggccagcatc
     3721 actatgtttg tcttcatctt cgtgattggt gtattgcatc aactggtgac acctatccag
     3781 tgggtaatga tgtccgatac cgtcgactac ggcgagtggt gcaatggtaa acgcctgacc
     3841 gggatcagtt ttgctggcac gctgtttgtg ctcaagctgg ggttggcctt cggcggcgct
     3901 cttatcggct ggatgctggc ttatggcgga tatgatgcgg cagaaaaagc gcagaacagc
     3961 gccacgatta gcatcattat cgcgctattc acgattgttc cggcgatctg ttacttgctg
     4021 agcgcgatta tcgctaaacg ctactactca ctcacgacgc acaatctgaa aaccgttatg
     4081 gaacagctgg cgcagggcaa acgccgttgc cagcaacaat tcacctctca agaagtgcag
     4141 aactaaggaa cggcaatgaa aattagcgac ggaaactggt tgattcaacc tggcctcaat
     4201 ttgattcacc cgcttcaggt gttcgaggtt gaacagcagg gtaatgaaat ggtggtctat
     4261 gctgcccccc gtgatgtgcg tgaacgtacc tggcagcttg atacaccttt atttacgctg
     4321 cgctttttct ccccacagga aggtattgtt ggtgtacgga ttgagcattt tcagggggcg
     4381 ctgaataacg gtcctcatta tccgctcaat attttgcagg atgtgaaggt cacaatcgaa
     4441 aacacagaac gttatgctga gtttaaaagt ggcaacttaa gcgcgcgtgt cagcaaaggt
     4501 gagttctggt cactggattt tctgcgcaac ggcgaacgta ttaccggtag tcaggtgaaa
     4561 aataatggct acgtgcagga cacgaataat caacgaaatt atatgtttga gcggctggat
     4621 cttggcgttg gcgaaacagt ttacggtctg ggagagcgct ttactgccct ggtgcgcaat
     4681 ggccagacgg tagagacctg gaaccgggac ggcggcacaa gtactgaaca ggcgtataaa
     4741 aatatcccgt tctatatgac taaccgtggt tatggggtac tggtcaatca tcctcaatgc
     4801 gtctcttttg aagtgggatc ggagaaagtc tccaaagtgc agttcagcgt tgagagtgaa
     4861 tatctcgaat actttgttat cgacggcccg acgccgaaag cggtacttga tcgttatacc
     4921 cgttttactg gtcgtccggc gctgccgccc gcgtggtcct tcggcctgtg gctaaccact
     4981 tcatttacca ccaactacga cgaagcgacg gtaaacagct ttatcgatgg tatggcggaa
     5041 cgcaatctgc cgctgcatgt tttccacttt gactgtttct ggatgaaagc cttccagtgg
     5101 tgcgattttg agtgggaccc gctgactttc ccggacccgg aagggatgat ccgccgcctg
     5161 aaagcgaaag ggctaaaaat ctgcgtctgg attaacccct atattgggca aaaatcccct
     5221 gtctttaaag agttacaaga gaaaggctat ttactcaaac acccggacgg ttcgttatgg
     5281 cagtgggata aatggcagcc aggtctggca atttatgact ttaccaatcc ggatgcctgc
     5341 aaatggtacg ccgacaaact gaaaggtctg gtcgcgatgg gcgttgattg ctttaagacc
     5401 gactttggcg aacgtattcc aaccgatgtt caatggtttg acggttccga tccgcagaaa
     5461 atgcataacc attatgcgta catctacaac gaactggtgt ggaacgtgct caaggacacc
     5521 gttggtgagg aagaagctgt cttgtttgcc cgctcggctt ccgtcggtgc gcagaaattc
     5581 ccggtacact ggggtggcga ctgttacgct aactacgaat caatggcgga aagcctgcgc
     5641 ggtggtttgt ctattggcct ttcaggtttt ggcttctgga gccacgatat cggcggcttt
     5701 gagaataccg cgccggcgca tgtttacaaa cgctggtgcg cgtttggttt gctctccagc
     5761 catagccgtt tacatggcag caaatcttat cgtgtgccgt gggcctacga tgatgagtcc
     5821 tgtgatgtgg tgcgcttctt cacgcaactg aaatgccgca tgatgccgta tctgtatcgt
     5881 gaagctgctc gtgcgaacgc gcggggtacg ccgatgatgc gggccatgat gatggagttt
     5941 ccggacgatc cggcttgtga ttaccttgac cgtcaataca tgttaggcga caacgtgatg
     6001 gttgcgcctg tgttcactga agcgggcgat gtgcagttct acttgcctga aggtcgctgg
     6061 acacacctgt ggcacaacga tgaactcgat ggtagtcgct ggcataaaca gcagcacagc
     6121 ttcctgagtc tgcccgttta tgtgcgtgat aacaccctac tggcgctggg caacaacgag
     6181 caacgtcccg attacgcgtg gcacgaaggc acggcattcc agctctttaa tctacaagac
     6241 gggcatgaag ccgtctgtga aatgcccgct gctgacggtt cggtgatctt tactctaaaa
     6301 gcaacacgta ctggtaacac gattactgtg actggtgcgg gcgaggcgaa gaactggaca
     6361 ctgtgcttgc gcaatattgt gaaagtaaat ggtctgcaag gcggttcgca ggctgaaagt
     6421 gagcaggggc tgatggtgac gcctcaaggg aatgcgctga caattacgtt gtaatctatt
     6481 gatggaatgc cgggtacgaa tggttttgtg tcgtatccgg taaataatct cacatctggt
     6541 aataaatatt tcttccatcg tatttgcgag catgatcacg attattcatc gcgaaaatgt
     6601 gatctgtgct gaaaaatatc ccttaaccgc cttgctttgt tttatcactg tccggattac
     6661 ggacagattt tagtattgtt gtgaaattat tctgctttat atttaccctc agagatggca
     6721 actgaagctc ttcctcatga ggcttcttaa tctgtgataa gggtaaaaaa tgcaaatgat
     6781 tacctcgcga caaaacagat tattacgatt ccttttacct cgaagggaat atacgactat
     6841 tgttacgatt gccggctatt taaatgtttc ggaaaaaacc attcaacgtg atttacgttt
     6901 acttgagcaa tggctgggac aatggagaat aaatgttgag aagcgtgctg gcgcgggtgt
     6961 gatgttaagc gcggagaata ttactgattt gctgcatctt gatcatttgt tggtggcaga
     7021 atgtgaagag attgatggtg taatgaataa ttccaggcgc gttaaaatag cgtcgcagtt
     7081 attaagtgaa acaccgaatg aaacgtcgat cagtaaattg tcagaacgct actttatcag
     7141 cggagcctct attgttaatg acctgagagt aattgagtcc tggcttgcgc cgttggggtt
     7201 atcgttgatc cgcagtccaa gtggtacgca tattgaaggt agtgaagggc aagtccgaca
     7261 ggcaatggca ttactgatta acggcattat taaccataat gagccgcaag gtgtcgtgta
     7321 ttcacgtctg gatcccggaa gctataaagc attagtccat tattttggag aagaagaggt
     7381 gttatttgtc cagtcattgt tactggatat ggaaaatgaa ttaagttggt ctttgggaga
     7441 accttattac gttaacattt ttactcacat ccttattatg atgtatcgca acacgcacgg
     7501 gaatgcgtta tcaagagaag aagatcaaac caggcaatat gatgaaaata tctttaatgt
     7561 tgccagtcag atgattcata agatagaaca acgaattgca catacattgc ccgatgatga
     7621 agtctggttt atttatcaat atatcatttc atcaggtgtg gcgattgatg gacaaaaaga
     7681 tgttagcatt atttcacata tgcaggccag caatgaagcg cgtctgatta cctggcgttt
     7741 aattacggta ttcagtgaca tcgtggactg cgattttagt gaagacagcg cattatatga
     7801 tggcttattg gtgcatatta aaccgctgat taaccgacta aattatcgta ttcatatccg
     7861 taatccattg ttggaagata ttaaagcaga actagcggat gtctggcggt tgacgcaata
     7921 tgtggtgaat caggtattta aaacctgggg tgagaatgca gtgagcgagg atgaagtagg
     7981 ttacctgacc gttcattttc aggctgcgat ggagcggcaa attgcccgta aacgcgtatt
     8041 actggtctgt tcaaccggaa tcggaacttc gcatctactg aaaagccgta ttctgcgagc
     8101 atttcctgaa tggacgattg ttgatgttat ttcagcagcg aatttatcac aggttttgcc
     8161 tgacaatatc gaactgatta tttcgaccat taatttgcct acagtcacta tgccggtcgc
     8221 ttatgttacc gcttttttta atgatgccga tattaagcgg gtcactgaaa tggtgattac
     8281 ggaaaaatta catcatgcga catctcgggt cgttgaaatt taatgtcaga attttatgag
     8341 gtatgacgaa tggacataac aaaaatcctg aatacgaatc gcgttatttt agatatgcaa
     8401 gcgacgaata aagaggaggc gattgaagaa ttaactaatc ttctcaaaaa agatggtgcg
     8461 atcgattgtc gggaaacctt tattaaagat gtctggcagc gcgaagcgga aggctcgact
     8521 ggttttgaaa atcatattgc gatcccgcat ggtaaatctt cagctgtgat taatactacg
     8581 ctggcaattg gccgtacccg gcaggatatt ccctgggaaa cgctggatgg cagtagcgtg
     8641 cgctgtatta ttttatttgc ggtacgtctt gaagatcaaa ataccaccca tatccgttta
     8701 ctttcccagg tcgcgggcgc gctggcggat gatgacatca tagaacaatt gttagttgag
     8761 acgagtccgc aaaaaatcat tgatctcttt agtcagtatg cacaatccga tgtctgctaa
     8821 ataacaggga gtatgtcatg aatattgtct gtgttgccgc ctgtacggcg ggtattgctc
     8881 atacctatat tgctcgtgaa aagttaatca aaggagcaaa agctctgggt cataccatta
     8941 aagttgaaac ccagggaacc attggtacag aaaatgaatt acaggcggca gatatctctg
     9001 ctgctgacgt cgtgattctt gctgtagatg tgaaaataaa aggagaagaa cgcttcacca
     9061 ataaacgtat tgtgcgagta aagacagaga tcgtcattaa atcgcccgtt caatttcttg
     9121 aaaaggtcga aaaatcactg ggaaacaaat gatccttata ctggagggat cttaatatga
     9181 aagaaaataa aattccggtt tcacaggaaa ttaaaaaaca tttattaacc ggcatctcgt
     9241 ggatgatacc ccttattgtg gccgccggga tatgtattgc gttggggcaa gttcttggtg
     9301 gtacagatgt tggtgaaaaa acaggaacaa tcccgtggat gcttaatcag attggcggct
     9361 ggggaatggg gctgattgtg ccgctgatta gcgcggcaat tgcctattct attgccgatc
     9421 gtcccggatt tgccccgggc ctgattgtcg gctttctctg tgggcaaatc cataccggat
     9481 ttattggcgg tatgttgggt ggtttcctgg tgggttacac cattttgcta cttaaacgct
     9541 atatccgcct gccgcagtcg atgcaaggac tcatgccaat catggtgttg ccggtattaa
     9601 gcaccattat cggtgggctg ttaatgatga cgctgattgg taaacccatc gcctggttac
     9661 aagaagcact gattcatttg ctcgagtcga tgcagggcgg atcgcgcttc ctgatggggg
     9721 ccatacttgg cgcgatggca accttcgatt tcggtggtcc ggtcaacaaa accatgtcgc
     9781 tgttttccga tggcttgctg gtcagtggcg tctacggacc agaagccgtg aaatttgtcg
     9841 gctcaatcat ccctccgttt ggcatcacac tttcgttctt actgacacgc cataaataca
     9901 cccgtgcgga gcgcgaggcg ctgaaagccg cgtttccgat ggggatctgc atgatcactg
     9961 aaggagtcat tccgatcgcc gcgcgtgacc tgctgcgagt agtgggatct tgtgtggtgg
    10021 catcggcggt cgcgggcggc ttaatcatgg tgtggggcgt ggagagtccg gttccgcatg
    10081 gcggcatgtt tgttgtgcct ctgttcaccc atcctctgct gttttgcctg gcgctgggta
    10141 taggcacggt gatttgtggc gtgatgcttt cactctggaa gaaaccggta acggaacgcg
    10201 acgaagagtt tgacgaactg agcgatcaaa agctgaaaga tgaagagatc actttcaccc
    10261 tcgaataacc ggaggcccta tgtttgccga tatgaaaagt atggtgatga aagcctggca
    10321 tgaacattac gctttgctgg cgatcaactg tatgaatctg gaaagtgctc acgcagctat
    10381 ccgcgtggcg gagaagaatc gcgctccgat cattctcaat ctctatcagg ggcatctggc
    10441 gcattttcct gcacccgtcg cggcggcagt ggttaaaacg ctcgcggaat ctgcctcagt
    10501 accggtcgct ctggcgcttg atcatggcaa aaatccggac tgtattcgtc aggcgtttcg
    10561 tgctggtttc agcggattaa tgattgatgc ttctgctttt cccctggagg aaaacgtgcg
    10621 ccagaccagg gcggtggtgg aactcgccgc cagcgtgcag ctctgcgtgg aaggcgaact
    10681 gggccatctg gcagatgcgc cgcgttacga ccaggccgcc aatgcagacc tgatgacgca
    10741 acccactgat gtggaaacgt ttatcgcgca gacaggcatt gatctactgg ccgtctcggt
    10801 aggcacggcg cacggcatgt acgcgcctgg tgttgtacct gctattgatt ttcagcgtct
    10861 ggcggagatt tcgcgctgct cgagcgtgcc tctggcatta catggcggta gcggtacgcc
    10921 gttcgatcag ctccagctct gtacgacgct gggggtagca aaaatcaacg tgggggcggc
    10981 catttttgaa cgggggaaat ccgcgctcct gcataccctc tgtcacgata tatccattga
    11041 actggtggac gcgctgaaag ctatggaact ggccttcgaa gaagcgatta caccgtattt
    11101 acaggcgagc ggcgctatcg gcaaagcctg attcattttc tgtcatggcc tttatgccag
    11161 tgcaataacg caccggcagg gcggactaca cgctgaaact ggagatttaa catgttagtt
    11221 tcgatgaaag aattactgca acctacccgc cagcacggct ttgccattgg cgcatttaat
    11281 gtggcggata actgtttcct tcgcgcagtc gtggatgaag cagaagcgac aaatactccg
    11341 acgattattg ccatccatcc cagcgaacat gattttgtgg gcgatgcctt tttcgctcac
    11401 gttcgcgaaa ttacccagcg tagtccggtc ccgtttgtac tgcacctcga tcatggtgcc
    11461 tctgtagagc atgttttgcg tgcgattcgc tgcggattta cctcagtaat gatcgacggt
    11521 tcgctgctgc catatgaaga aaacgtggcg ctgaccgcag aagtggttcg cctcgctcac
    11581 gcggttggag tatcggttga gggtgaactg ggaaccatcg gacaaacagg aacgtcggta
    11641 gaaggtggtg tttcacaagt gacctacaca gatccggcgc aggccgccga ttttattgct
    11701 cgtacaggtg ccgacacact ggctgtggcg attggtacag cgcatggcat ctatccaaaa
    11761 gggatgcagc cgaagctgca aatggacatt ttgcgcgaca ttgccggacg tattgatatt
    11821 ccgctggtgc tacacggtgg ttcggcaaac ccggacgcgg agattgctga atcagttacg
    11881 ctcggcgtcg gcaaaatcaa tatctccagc gatatgaaat acgcctattt ccagaaagtg
    11941 cgtgaaattc tcgctaaaga gagctggtgg gacccgaacg tcatctatcc ggatgcgatt
    12001 aacgcagcgc gggaagtcat tcgccacaaa atgaaactgt tcggttcgct cggtaaagca
    12061 tcactgtact aagccagaag ggcggggtga cccgcctttt acgcaacaag gaacgagata
    12121 tgcactatct ggggttggat attggtggga ccaaaatcgc cgccgtcgtc atggatgcgc
    12181 atggctggga gattcgccgt taccgctgcc cgacgcaaaa gtcgacatat caacaatttg
    12241 tctcatgcgt tgtggcgctt atcgagcaga ttaggcggga cgttcaacga ccgatgctga
    12301 cagggatcgc cttacccggc agtatctcgc cactcactgg cctgattaaa aacgcgaata
    12361 ttcaggtgat taacggtcat gcgttacagg ctgatttgca gcaattgctt gggcaaccgg
    12421 tggtgatagc caatgatggt aactgtttcg cgctatcaga agcttgcgac ggtgccgggc
    12481 aagattatga cgtggtattt ggtattacgc ttggtacggg ctgcggcggt ggcattgcca
    12541 tcaagcaacg accgtttata ggggcctggg gaaatgctgc cgaatgcggt catatcacgt
    12601 tgccaggcta tacggagcag gaagatggtc catcagtcag ttgctattgc ggcaaacaca
    12661 actgcgtgga gtcgtttgtt tccggcagcg gttttagtga acgctatcaa cagatgactg
    12721 gtaacttgct cactcctgcg gcgattgtca ccctggcaca acgtggtgat gcttgtgcca
    12781 tgcagcaggt ggcacgtttt cgccaacagc ttgcccgcac gctggcaacc atcgttaatg
    12841 ttgttgaccc tggcgtgatt gtgatcggcg gcgggctttc gaatgtggaa ctgcttatca
    12901 ccgatctgaa cgcagaagtc gctccactgg ttttcaccga tcaattcacc actcccattg
    12961 taaaagcaca gcacggcgac agtagcggaa tgcgtggcgc tgcctggctt gctatgcgca
    13021 acggagaagc caatgaaacg ttcacaaatt aattatgtta ttgataaagc acatgccatt
    13081 gcgcagacct tccgcgtttg tttgccagag ttcgcctatt ttacggtgga tgactggctg
    13141 cagcgggaac gggataactg gcaagaagtg gtcgacttac agctcggttg ggatatcacc
    13201 gattttggtc gtggtgattt caaccaaacg ggactaacac tgctaacgat gcgcaacggc
    13261 gcgctggggt cggcggcata tccaaaaccg tatgctgaga aaatgttgca gatccaacaa
    13321 gatcagcaga ctccctggca ttttcacacc cacaaaatgg aagatatcct caatcggggc
    13381 ggtggagact tatgcatgca gttggcctgg gccaacgaag ccaatctctg tgatgatcaa
    13441 cgagtcatta ccgtgagtgt tgatggtcag cggcgtacca tgaaacctgg tgaaacgctg
    13501 gtgctaaagc cggggcaggg catttgcctg ccgccgcgtc tgtatcatcg cttctgggcg
    13561 gaaaaagcgt ttgttctcgg ctgggaaatc tcgatggtaa atgatgacca acacgataat
    13621 tattttcttg agccgggagg acgttttccg gcgatagaag aagatgaacc ggtgaaatgg
    13681 ttattgtgca gtgagtatgg aattttgcgc taataacggg gaacgctagc gctgatatcc
    13741 gctagcgtcc ttatttttac tgcaccggct ccgtcgacgt aacttcacca ctggaaacta
    13801 cgcccgcatt catggtttga gtcacttgct gctttgcggc attcacggca tgcaattgcc
    13861 cggtaaccgt aggtttcagc gggacattgg cctgaatatc gccactggca gtaagctgaa
    13921 tattgccgtc gccagtcaac ggtaattcag gccatcccca ctgttgcaaa atattcaccg
    13981 gcacaccgcg tccattcagg ctgatatggg tctgacgttg tggcgtttgt gaaacactgg
    14041 cggtggcttc cagaatgcct ttttcagtaa atgcactcag ttcgctgata ttcaccgtgc
    14101 tgctgttggc ggtcagcgcc agcgatgggc gacgaacatc aacacgattg aatgtcgcgg
    14161 cggcggcatt cagattcgcc gaaccactcc agacgcccca tttatgatcg gtaaccagcg
    14221 tcaggttggc accgtaacca tcgagcgcgg tgagctgcca cgggaagtca gggtcgatat
    14281 caatgatcag attgcggctg gcgctaaatc tcttcagttg caggctattt aaccaaccgg
    14341 gtgtcgtttc catccacaac tgttgccagt tttttggcaa ggtatattcc agcccggcaa
    14401 ttgccgcatc atcaaggagt aacgttttcc cgtcacgcaa ccagctccct gacgttctga
    14461 ccataccccc ttcccagcgg ctggtgaact ggcgcagcgc tacgccctgc ggggaaaatt
    14521 ccgcgtttat aatcgggtca aataaatgca gcgaaccata aatgaactcg ctagcgttca
    14581 tcgacagttt gccttcctgt gtctgccagt catctttact gaaggtcatg ttgcgcaagc
    14641 tgagatcgag gtcggtcacc gcccagtccg gaccttgcaa acgagcgtca atgacttcca
    14701 ggcgaccaat ctgcaacgac gggacagagc gtaatggcgc aaagaagtcg gttagcgatt
    14761 tttcgctttg tagacggata tccgccatgc gcagattttc cacttgccag ctgccgtcgg
    14821 cgttacgctg cgcgtttccg gttaatgtcc cgcgggcgat gtcggcaccc aagttagtca
    14881 gcgtaacgcg atcgttatca atactgcctt caatcagtac attggtggca ggaacatcat
    14941 tgagcgaaag cgatccggca ctaaactgaa tctgcgcctt cgtacccagc actttaccgg
    15001 cttctggtga ccacggaacc acgccaccat ttacccgctg cgcgcttagt ttccattcgc
    15061 tattcgggct attaaacgcc atatcacgca gttgcagacg atcggctttg aacggtagcg
    15121 gcgcggtctg gtcggtgaga ttcagcgtcc cgttttccag caagatggta tcgacatggc
    15181 gtggttcggt cagttggcga ctgcttagcg caatgtcgac acttttggcc accagggtcg
    15241 cgggctgacc atcacgacca aacgtgacgt tctccagcac gatatgagat ggcgcggaaa
    15301 aacggtgatc catcgccccg aaggccagat gatagtcgct attctcggaa acccatgcgc
    15361 tgatatgttc tgctccccag cgggtttgca gaagaaaata gaggccagcg atcaccacta
    15421 acagagcgat gagaatgtag agaagcagct tcccaataaa tttcatggtc ttccatcccg
    15481 tgaaaagcac ataagggagt tatgcacgat ttacgcgcaa tcctcaaggc gggaatggtg
    15541 aaagacgtga gactgcggcg gtaattatct taccgccgcc agtgaattac tgtttttctg
    15601 gtgggaaaat cagattcagc acgatggcag taataccgcc cgcggcaatc ccggaggaga
    15661 gcaggttttt cagccattca ggggcaaact gcaaaatcag cggctgctga gagacgccca
    15721 gaccgaccgc cagcgacagc gcgataatca gaatcgcccg acggttcagc ggctcacgag
    15781 aaacgatacg cacaccggag gcggcgatgg tgccaaacat tacaagcgtt gcgccgccca
    15841 gaaccggttc aggaatgtgt tgtacaaaac cgctcactgc cgggaacaga cccagcacga
    15901 tcaacatcag cgcgacgaca aaaccgacat agcggctggc aacaccggtt aactggatca
    15961 ctccgttgtt ctgcccgaag caggagttcg ggaaggtgtt aaacaccgcc gaaacaaacg
    16021 agttcaggcc gtttgccagc acaccgcctt tcaggcgttt catgtacagc ggaccggaca
    16081 ctggctgttc ggaaacgtca gaggtcgccg tgatatcgcc aatggtttcc agcgaagtga
    16141 tcataaagac cagcatcagc ggcagcagca gactccattc aatgccaaga ccgtaataga
    16201 gcggcgttgg caccataatc agttcttgtg tcatcggttc gttgctttct ggcaacatgc
    16261 ccataaacca cgccagcgca tatccggccg ccatcgcaat taccagtgag gccacgcgta
    16321 agtaagggtt acgttgacgg ttaagcagga taattaaggc taaaaccacg cctgccagca
    16381 gcagattttt cggtgcgccg aaggtgttat cgctcatggc tgcgtaaccg ccgccaatgg
    16441 acgttaaccc aacctgaatt agcgacaggc cgataatcat caccacaacg ccagaaacta
    16501 gcggcgtaat aatgcggcgc gccagatgca gaacgcggga gatcaccatc tcagtgcaac
    16561 ttgccagcat caacgtgccg aacaaagccg ccatcatggt aggaacatca gcaccaccgg
    16621 ttttcagcgc ggtaccgccc ataatcagtg gggcaacaaa gttgaagctg gtgccctgaa
    16681 tagacaacag cccggagcca accggacccc aggccttaat ttgaataatc gatgccacac
    16741 cggaggcaaa cagcgacata ctaataatgt gttgcgtgtc ttgtgccggt aaacccagcg
    16801 cctggcagat taatagcgct ggcgtgatca ccgcaacgaa catcgccagc agatgctgac
    16861 aggcggcaaa cagagtttga ggaagcggcg gacgatcttc aagacggtaa atcagttcgc
    16921 tgttttgagt ctgcgcaacc ggttgcgcat tttctgactc gagggtggaa acagacatcg
    16981 aaaacaatcc cgttgtagca aagcgggcat tttagctgac cgaaagagaa aagcaaacgt
    17041 ttgccacggc ctctgctaaa tatttaaatg agataaatgc acttttttat ataacttttg
    17101 gtatttttct gcttaaaatc catgccattt gttcattaac agctgagaat ttgacccagg
    17161 aaagcataac tcgatacccg tcatagttca taagttgcca ctgcgcgatg aagtatgacg
    17221 agtatgaaag agtgatgcgg atacaaagga gtaactatgt ttcatctcga tactttagca
    17281 acgcttgttg ccgcaacgct gacgttgctg ctcgggcgta agttggtcca ttccgtctcc
    17341 tttttgaaga aatacaccat accggaacct gttgcgggtg gtttgttggt ggcgctggcg
    17401 ctactggtac tgaaaaaaag catgggctgg gaagtcaact ttgatatgtc cctgcgcgat
    17461 ccgttaatgc tggcattctt cgccaccatt ggcctgaacg ccaacattgc cagtttgcgt
    17521 gccggtgggc gtgtggttgg catcttcttg attgtggttg ttggtctgtt ggtgatgcaa
    17581 aatgccattg gcattggtat ggctagcctg ttagggcttg atccgctgat ggggctgttg
    17641 gccggttcta ttacgctttc cggcggtcac ggtacgggcg ctgcgtggag taaattgttc
    17701 attgaacgct atggcttcac caatgcgacg gaagtggcga tggcctgtgc gacgtttggt
    17761 ctggtgctgg gcggcttgat tggcggtccg gtagcgcgct atctggtgaa acactccacc
    17821 acgccgaacg gtattccgga tgaccaggaa gtcccgacgg cgttcgaaaa gccggatgtg
    17881 ggccgcatga tcacctcgct ggtgctgatt gaaactatcg cgctgattgc tatctgcctg
    17941 acggtgggga aaattgttgc gcaacttttg gctggcactg cttttgaact gccgaccttc
    18001 gtctgtgtac tgtttgttgg cgtgattctg agcaacggtc tgtcaatgat gggcttttac
    18061 cgcgtctttg agcgtgcggt atccgtgctg ggtaacgtaa gcctgtcgtt gttcctggcg
    18121 atggcgctga tggggctgaa actatgggag ctggcttcgc tggcgctgcc gatgctggcg
    18181 attctggtgg tacagaccat cttcatggcg ttgtatgcca tcttcgttac ctggcgcatg
    18241 atgggcaaaa actacgatgc ggcagtgctg gctgcgggtc actgtggttt tggcctcggt
    18301 gcaacgccaa cagcaatcgc caacatgcag gcgatcactg aacgttttgg cccgtcgcac
    18361 atggccttcc tggtagtgcc gatggtcggt gcgttcttta ttgatatcgt taacgcgctg
    18421 gtgattaagc tgtatttaat gttgccgatt tttgccggtt aaccgatgaa gcggcggtct
    18481 tctcaccgcc gccttttacg cattcgagta acgttccgtt tctggcatcc agcgttctat
    18541 cagggctttt gcctgctgtg ggtaacgttc gtgaatatgg cgtgccaggc gctgaacttc
    18601 cgggatcatc gcctgatcgc gcagtaaatc cgccacttta aattcagcat tacccgtctg
    18661 acgcgtgcct aacaattcgc cagggccgcg aatctccaga tctttttgcg caatcacaaa
    18721 cccgtcgttg ctgtcgcgca gtacttgcag acgaatttgc gccgttttag agagcggcgt
    18781 tttgtagagc agcacgcagt gagaagccac cgcaccacgt cccacgcgcc cgcgaagctg
    18841 gtgcaactgc gccagaccca gacgctccgg gttttcgata atcatcaggc tggcgttagg
    18901 cacatcaacg ccgacttcaa taacggtagt ggcaaccagc aggtgtaact cgccttgttt
    18961 aaatgatgcc atgacggcct gcttctcggc tggtttcatc cgtccgtgta ccagaccaac
    19021 attcaactct ggtagcgcca gtttcaactc ttcccaggta gcttccgccg cctgcgcttc
    19081 cagtaactct gactcttcaa tcaacgtaca aacccagtat gcctgacgac cttcagttat
    19141 gcaggcgtgg cgcacgcggt caataatgtc ggtacggcgg gtatcaggaa tagcgaccgt
    19201 agtcactggc gtgcggcctg gcggcagctc atctatcacc gaggtatcga gatcagcata
    19261 cgcagtcatt gccagcgttc gggggatcgg cgtggcggtc atgatcaact gatgaggatg
    19321 gaagccttgc tgctggcctt tctcccataa cgccagacgc tgatgcacgc caaaacgatg
    19381 ctgttcgtcg ataatcacca gcgccaggcc gttaaactgc acctgttcct ggaagatggc
    19441 gtgtgttccg acaatcatct gcacctgacc gctggcgatg gcttcctgct gtgccagtcg
    19501 cgctttacct ttctgcttac cggcgagcca gcccacttcg ataccgagcg gtgcaaacca
    19561 gttgcggaag ttattcgcgt gctgttcggc gagtaattcg gttggtgcca tcaatgccac
    19621 ctgtttgccg tgcgcaatcg cacgcagcgc ggcgagggca gcgaccagcg ttttaccgga
    19681 acctacatcg ccctgtacca ggcgcatcat cggcgcatcc agcgccatat cgcgctcgat
    19741 ctccgccacc acgcgtgcct gtgcgcccgt tggcttgaac ggtaaggctg cgaggagttt
    19801 atttttcagc gcgtcattgg cgctaagcgg ctgggcatgg aaacgttgtg caccagcgcg
    19861 taacgccaac atgctgaggt tgtgcgcaag cagttcttcc agaatcagac ggcgttgtgc
    19921 tggatgctgc ccggtttcga gatcgctaag ctgtagcgtc ggcggcgggc ggtgcaaagt
    19981 gcgcaacgct tccggtagcg tcatcattcc ttgtgacagt tccggcggca ggagttcttc
    20041 aatggcgcag gtgtcgagca gatccagcgc ctgatcggtt aatttacgca gcgtggcctg
    20101 ctttacgcct tccgttgttg ggtaaaccgg cgttagcgtt tcctgcaact ctggcgtact
    20161 gagatcgccc tgcacgcggt attccgggtg gatcatctcc gcaccatatt taccgcgctt
    20221 tgcttcgcca taggccagca cgcggcggcc tgtcgccagg ctatttttca ttgccgcgtt
    20281 gaagttgaaa aagcgcatgg tgagaatgcc ggaaccgtcg ctgatctggc aggtcatcat
    20341 ccgccgaccg ccgaaggaga tattgcagtt cagcacttcg ccttccaccg tggcataaac
    20401 gcccggtagt agttctccga tggggtagag atgggtgcga tcttcgtagc gcaggggaag
    20461 gtgtaagagt agatcctgca cggtatgcag gttgattttc gccagcttgt tactaagcgc
    20521 tgcgccaacg cccgttaggg aactgagcgg gacagcatct aacaggcgac ctttcatggc
    20581 acttaccctg cagcctgcat ggtagcccac cagtcagcat cggcttcaat ttcgccttgc
    20641 tgattaacgt gggggtaagg caggcctttg cgttttgcga ctttcgccag caccggatag
    20701 ccgccttcaa acagcaggcg ttgttgctct gcttccggca acatgctgtt ttcacgcagg
    20761 tacatgcctg cattttgccg ctggcgctgg gcttcataaa gaatgagggc tgaggcaacg
    20821 gaaacattca gcgactgcac catgccgatc atcggtatga tgatgtcccg atccgccagg
    20881 gccaacgctt cctgcgtgat gcctgttttc tcctgtccca tcaaaataca ggtcgggcga
    20941 gtgtaatcaa tttcgcggaa atcgacagcg ttatcagaaa gatgggttgc cagaatctgc
    21001 atgccctggc ctttcagatg agcgacggca tcgccaatgg tgcggtgcgt tttcacctgt
    21061 acccagctgt tactacccgc agctgccgaa gccatggtgc gcatccggct accaggccag
    21121 acagcatgaa cttcatgtac accaacggca tctgcggtac gaataatcgc agaaacgtta
    21181 tgaggtttgt ggacctgctc catgcagacg gtcagatcag gctgccgcct ggcgagcatt
    21241 tcgcagatgc gtgcataacg tgttgggttc ataaaacatt aatttcggtt tcgggtgact
    21301 ttaatcacgt ctggcatcac gcggattttg cgcatgatat tcgccagatg cacacggtca
    21361 cgggcggtca gacgaataaa ggcgctgtag acgcgaccat ctttctcttc cgtattcaaa
    21421 ctttgaatat tcgaggtggt ggtgttaatt gccgctgtca ggtttgccag cgcaccctga
    21481 tgattgaaca tctccacctt gatttcggtg atgaactcct gcgccgtctc tttatcccat
    21541 tccacagcca taaacttctc tggctctttc tggtagccac ggatgttacg gcaggattca
    21601 tggtggatca ccagaccttt accggggctg acgtgggcga taatcgggtc gccaggaata
    21661 gggcggcagc atttcgcaaa ggtgatcagc acgccatcgg cacctttaat gggcagatgt
    21721 ccgtgacttt gggttgccgg tggaatggag gcgtccccat gttgcagatt tttcgcgacc
    21781 accacgctca ttgcgttacc aaggccgatt tcagccagca gatcgtcaag cgttgccagc
    21841 ttcatgcggt ccagctcgcg ctgaatattt tcctgcggga tttcattgag cttgcggctg
    21901 ccacccaacg catggttgag cagacgacgg cccaggctta cagaatcatc acgcttgagg
    21961 tttttcagca attgacgaat tttggcgcgc gctttcgagc taacgacaaa gttcagccac
    22021 gcggcattcg ggcgagcgcc cggtgcggta ataatttcaa cggtttgacc gctggtaagc
    22081 ggctgcgaca gcgggtaagg ctggcggtcg acgcgtgcgc ccacgcaggc atgaccgata
    22141 tcggtatgca ccgcataagc gaagtcgacg ggcgttgcac cggcaggcag ttcgacaatg
    22201 cgcccttctg gtgtgaaaac gtaaatctca tccgggaaga gatcggattt aacgctctcg
    22261 ataaattcaa acgagctacc ggcactctgt tgcagctcca gcaggctttg catccagcgc
    22321 tgggcgcgta tttgcgcggt agtgctggtt tcgccgtgct ctttataagc ccagtgcgcg
    22381 gcaacaccca tctccgccat ctgatccata tcttcggtac ggatctggac ctcaaccggc
    22441 acgccgtgcg ggccaatcat cgaggtgtgc aaagactgat agccgttcgc ttttggaatg
    22501 gcgatatagt ctttcacgcg gcccggacgc ggcttgtaca ggctgtgcat ctggcccagc
    22561 acgcgataac aggtgtcaga atcattgacg atcacgcgga aagcgtagat gtccatgatt
    22621 gagtgaaaac gctgctcttt gagcaccatt ttgcagtaaa tcgaataaag atgcttttcg
    22681 cgaccactga cgcggcacgg tattcccgct tcctgcaaac gcccttcgat ttcagaaaga
    22741 atcttctgga tcatctcttt acggttgccg cgcgcggctt tcaccacttc tttgattacg
    22801 cgataacggt tgggatacag cgcctcaaaa cccagctctt cgagttcggt tttaatgtgg
    22861 tggataccta aacggtgcgc cagcgggctg taaatttcga gggtttcacg ggcgatgcgg
    22921 cgacgtttgt ccgggcgaag tgagcccagc gtgcgcatgt tgtgggtacg gtcggcaagt
    22981 ttgatgagaa tgacgcggat atcctgcacc atcgccataa tcatcttgcg aaagttttcg
    23041 gcctgcgcct ctttcttatc gcggaacttg agcttatcaa gtttcgacac cccctctacc
    23101 agctcggcga cgcttttacc aaaaagctgt tccatgtcct ggtaggtggc gggagtatct
    23161 tcaatcacgt catgcagcag cgccgccata agcgtttcat agtcgagttt catctcggcc
    23221 agaatgcagg caaccgccac cgggtgcgtg atatagggtt caccgcttga acgtgtttgc
    23281 ccctcgtgag catcacgtgc aacgagatac gcctgccgca gacgcttgat ttggtcttcc
    23341 ggcaggtagg tttgaatcag ttgattcagg ctttcaaaca gatacaaggg cgacccgctt
    23401 tgtgattaac gacgaccttc agcaatagcg gtaacggctt gtaattcagc ggcttcctgc
    23461 tcttgctgtt cctggcgttc gcgaacgtcg aggatctggt tgttgatcag accttcttcg
    23521 atttcgcgca gcgcgattac agtggtttta tcgttttctt ccggtaccag cggatccttt
    23581 ccgcctacct gcatctgacg agcgcgacgc gcggcgacca gtaccaggtc aaaacggtta
    23641 ccaattttct ctacagcgtc ctgaacagtt acgcgtgcca tacttaaaaa gctccacagg
    23701 tgaagaaatg actgggcatg atactgaaat caggttcagt ctgccaacaa tttgctgatt
    23761 aaagcgtcat gacgctgctt ttggcggctc atgcgcagac gttcggcgcg aataatggtc
    23821 ttcaaatcgg tcaacgcggt atcgaagtca tcattcacaa tcaggtaatc atattcggcg
    23881 taatggctca tttctgcaac agcttgcgcc atacgctttg caatgacctc ttcgctgtcc
    23941 tgaccgcgac cgcgtagacg gcggtccagt tcaattttgg acggcggtaa aataaagata
    24001 ctccgcgcgt gcggcatctt ctggcgaatt tgctgcgcac cctgccagtc gatatcgaga
    24061 aaaacatcga caccggtcgc cagtacttgc tcaatggcct cacgcgaagt gccatagtaa
    24121 ttaccaaaaa cttctgcgtg ttcgaggaac gcatctctgc taatcatttc tttaaattca
    24181 tcatgattaa caaagaaata atgttcaccg tggacctcac caggacgcgg ttggcgtgtg
    24241 gtgtgtgaaa cagaaacctg ggtgtcatac aacggttggg tttttaataa agcctgaatc
    24301 aggctggatt tacccgcgcc actgggggca gaaacaatat aaagcgtgcc ttgagccatg
    24361 agtatctttc gtatgtgatt agcgaaataa agcctacata cgagcttatt atacacggcg
    24421 cggccatgtg acgtagccct tgctcacact ttttgcgcaa agttgttgcg ttttgcgtag
    24481 ctgcttccgc atttacctgt cattcactgc aataaagacg attctcagga agcgtcctcg
    24541 cagactccgt ttttgccact cggctttgct ttcatcacgt tgtatatctg cctgactatc
    24601 tacagcggag gtgatgatga aagtatggat ggcgatatta ataagtatct tgtgctggca
    24661 atcatcggcg tgggcagtct gtccggcctg gtcgccagcc agagcacagg aagaaatttc
    24721 ccgcctgcaa cagcaaataa aacagtggga cgatgactac tggaaagaag ggaaaagtga
    24781 ggtggaagac ggtatttacg atcagttaag tgcccgtctc acgcagtggc aacgctgttt
    24841 tggtgaagag acgcatcgcg acgctatgat gccgccttta aacggtgcgg ttatacatcc
    24901 ggttgcacat acgggtgtac gcaaaatggc ggataaaatc gcattaagtc tgtggatgcg
    24961 cgaacgtagc gatctttggg tgcagccaaa agttgatggc gttgcggtaa ccctggttta
    25021 tcgggacggg aaactgaaca aagcaatcag tcgcggcaat ggcctgaaag gcgaggactg
    25081 gacgcagaaa gttcgcttaa tttccgctgt gccgcaaacc gttagcgggc ctttagccaa
    25141 cagtacgctt cagggggaaa tatttctcaa acgcgagggg catatccaaa aacaaatggg
    25201 gggaataaat gcccgcgcaa aagttgctgg tctgatgatg cgccaggacg atagcgacac
    25261 gctgaattct ctgggcgttt ttgtctgggc atggccggat ggaccgcagt taatgaccga
    25321 tcgtttaaaa gagctggcta ccgctggttt tactctaacg cagacgtata cccgtgcggt
    25381 gaaaaatgct gatgaagttg cgcgcgtacg caatgcgtgg tggaaaacga aattaccctt
    25441 cgtcaccgat ggcgtggttg tgcgagcggc gaaagaaccc gaatcccgcc attggctacc
    25501 aggccaggca gaatggctgg tggcctggaa atatcaacct gtagctcggg ttgccgaagt
    25561 gaaggcgatt cagtttgcgg tgggtaagag cggtaaaata tcggtggttg cgtcactcgc
    25621 acctgtcatg cttgatgata aaaaagtcca gcgggtgaat attggttccg tcaggcgctg
    25681 gcaggagtgg gatattgcgc ctggtgatca gatcctcgtc agccttgccg gtcagggtat
    25741 tcctcgcatt gatgatgtgg tgtggcgcgg tgcagaacgt acaaaaccga caccgccaga
    25801 aaaccgcttt aactcgttga cctgctactt tgcttctgat gtttgtcagg aacagttcat
    25861 ttcacgctta gtctggctgg ggtcaaaaca ggttcttggg ctggatggca ttggtgaggc
    25921 cggctggcgc tcgctgcatc agactcatcg ctttgagcat atcttttcct ggcttttatt
    25981 aacgccagag caattacaga acacgccggg gatcgcgaaa agtaaaagtg cgcagctatg
    26041 gcatcagttt aatctggctc gtaagcagcc ttttactcgc tgggtgatgg caatgggaat
    26101 accgctaacc cgggcggcgc ttaatgccag tgatgaacgg tcctggtcgc aacttttatt
    26161 gagcacggag cagttctggc agcagctgcc ggggactgga tcggggcgtg tcagacaggt
    26221 tattgaatgg aaggaaaatg cgcaaatcaa gaagctgggc agttggctgg ctgcccagca
    26281 gatcacaggt tttgaacctt agtgtccttc gtggctgtag taaaagaccg gtaatcccag
    26341 cttaaggcgt agcgccagta agcgggcaaa aaagccgaat accagggtgg agataattac
    26401 cacatcatgg ttagaaacat agtgttgcag cgcgatgtac agcacggcgg aggcaaatga
    26461 gacaccggca tacaactctt tctggaatac cagcgggatg cgtttacaga acatatcgcg
    26521 caaaacgccg ccaaatacgc cggtcgtcac agctgcgaca acggcaatga tcgggccgtg
    26581 tcccatatcc agtgcaacct gtgcgccaat gatagaaaag accaccagtc cgagcgcatc
    26641 gagcaccaga aacactttgc gcaggtaagg cattacagga gcaacgatag tggtgagtac
    26701 tgcggcggta gcgacaataa tcacatattc cgggtgtttg acccagccga gcggatagtg
    26761 gcccagcaga atatcgcgca ctgaccctcc gccaattgcc gtggcggtcg caataataat
    26821 tacgccaaat gtatccatgc gccgtcgtcc ggccgccagc gcccccgtca tggcttcggc
    26881 agtaatgcca accaaataca aaatgtgtaa cagcatggtt ctctccctaa aaatctgtct
    26941 ggcagggtag cgatttgtgc gtattgtcac gattgagatt ttttaaggtg agaggaaatt
    27001 gataacttgt agttatgtga aatcgctatt ttctgtagcg tagatgcatt cttctgactt
    27061 cttgtctgag aaaatgcata agaaaaaata ggttaaaatt gaggggtgga aaagaaaggt
    27121 gcaatgttta gccattgtgt tcaccgcacc tggactttgg cttacgcatc gggtatctca
    27181 atcaacaaaa agagttttta gtttctgcta tttcttctct gtaattttta cactgttttc
    27241 caactgcttg atgttgacct gcggggtcgg caactcctca ggcatggttc cgccaagttc
    27301 ctgaatggtt tgcctcacct tgcgacccac gtcaaagtgt gtggtgttag cttgctgttt
    27361 tgaattcacg ccatcccgct tgagtttttc ttctgtttgg gtagctcgaa agagattagc
    27421 cgccagttct gttgaaccca tatgatccag gatcttctga ctctttttca gccccttccg
    27481 ctgatggatg gctttctgat ctaatccacc atacagcccc cggtaaccat gattctgaaa
    27541 tatggcgaaa tcgatagctg tcgctacccc agcttgctgc gctgcctcaa ctaattgttt
    27601 gttatgttct ttcaactcat ttctcagaaa aagacgcttt tcgtcttcac gaagttgcct
    27661 gaaagcttca tcgtcagcca gctcctgccg tcgggtctgg atagcaaaat aagtttgccc
    27721 tgccgcaata accggtttcg cagggtcgcc gttttgtact accaggtaac aggcatagcg
    27781 ggagagtacg atgtctttta atgctctttt ggcatttgag ccaattttga ccatatcgag
    27841 gatctcctcg aaatggtcat caattgtgtg accactattc agacaagctt ctttggcgcg
    27901 cgttaataca ggtataaagt gacgatactc tgaataaccc agtagttttc caagttgcct
    27961 tgctgaccat tgttctgctc cgtttgcatt aatcagcttt atctcttcaa aaggttgatg
    28021 atgttcgttc attgcccact ccatgtgata tcgattgcaa ggaaagtaag tctacaactg
    28081 tgtttatata cagttgtaag tatctcacgt tctcactgcg ctgggcaaaa aaacatcgca
    28141 aaatctggag caggcacgca aaacagaatt gaatgttgcg gggaggaaaa atgccgctct
    28201 gtttaagtgg gcggcatttg tgaaggctga aaaggaagaa ttggcgttac gagttattca
    28261 atgttctgga tctgctcgcg catctgctca atcaacactt tcagctcgat ggcagaattc
    28321 gtcacttcgg cattgataga tttcgacgca agagtgttcg actcgcggtt gaactcctgc
    28381 atcataaagt ccagacgacg accaaccgct tcttttttct tcagaatgtt gtaggtctct
    28441 ttgacatgcg cttcgaggcg gtccagttct tcggcaacgt caattcgttg tgccagcaga
    28501 accaactcct gctccagacg gttgttttcc agttgcacct gagcatcttc cagcttcgcg
    28561 accagacgct cacgctgcca ttgcaggatt tccggcatat gggcgcggac tttgaccact
    28621 tcggcggtga cgccttccag acgctgctcg atcaatgctt tcagcgcctg accttcggtt
    28681 tcgcgcgcga cgataaagtc gtccagcgta ccatccaacg ccgcgagaat ttcagcggca
    28741 atcgcgtcga gatcctgctc ctgggctgcc atcacgcccg gccagcgtag aatatcaacc
    28801 gggttgattt ccccttcgtc actctgcatt tttacccagt tcgcggcagt taccagctgt
    28861 ttagccagtt tttcgttgag gatcagctca ccttgcgcgc taacatctgg ctcatagcgc
    28921 agggtacatt ccactttacc gcgcgtcagg cgagaacgaa tacgctcacg aacgacaggt
    28981 tcaaggctac ggaactgctc cggcagacga aagtaagttt ccagataacg ctggtttacc
    29041 gagcgcattt cccaggttgc gctcccccat tcacccttga tttcacgccg ggcgtaggcg
    29101 gtcatactgc ggatcataga cgttcctgtt tataaaagga gaggtggaag gattatagcc
    29161 atcgatgcct tgtcaggata ggaataaccg ccggaagtcc gtataatgcg cagccacatt
    29221 tgtttcaagc cggagatttc aatatgcgtc cagcaggccg tagcaataat caggtgcgtc
    29281 ccgttaccct gactcgtaac tatacaaaac atgcagaagg ctcggtgctg gtcgaatttg
    29341 gcgataccaa agtattgtgt accgcctcta ttgaagaagg cgtgccgcgc ttcctgaaag
    29401 gtcagggcca gggctggatc accgcagagt acggcatgct gccacgttct acccacaccc
    29461 gtaacgcgcg tgaagcggcg aaaggtaagc agggtggacg caccatggaa atccagcgtc
    29521 tgatcgcccg tgctcttcgt gcggcagtag atttgaaagc gctgggtgag ttcaccatta
    29581 cgctggactg cgacgtgctt caggctgatg gtggcactcg taccgcgtcg attacgggtg
    29641 cctgcgtggc gctggcagat gcgctacaga agctggtgga aaacggaaag ctgaaaacca
    29701 acccgatgaa agggatggta gccgcagttt ctgtcggaat tgtgaacggc gaagcggttt
    29761 gcgatctgga atacgttgaa gactcagccg cagagaccga catgaacgta gtgatgaccg
    29821 aagacgggcg catcattgaa gtgcagggga cggcagaagg cgaaccgttc acccacgaag
    29881 agttactcac cttgttggcg ctggcccgag ggggaatcga atccattgta gcgacgcaga
    29941 aggcggcgct ggcaaactga tttttaaggc gactgatgag tcgccttttt tttgtctgta
    30001 gaaaagtaag atgaggagcg aaggcatgaa accatatcag cgccagttta ttgaatttgc
    30061 gcttagcaag caggtgttaa agtttggcga gtttacgctg aaatccgggc gcaaaagccc
    30121 ctatttcttc aacgccgggc tgtttaatac cgggcgcgat ctggcactgt taggccgttt
    30181 ttacgctgaa gcattggtgg attccggcat tgagttcgat ctgctgtttg gccctgctta
    30241 caaagggatc ccgattgcca ccacaactgc cgtggcgctg gcggagcatc atgacctgga
    30301 cctgccgtac tgctttaacc gcaaagaagc aaaagaccat ggtgaaggtg gcaatctggt
    30361 cggtagcgcg ttacaaggac gcgtaatgct ggtggatgat gtgatcaccg ccggaacggc
    30421 gattcgcgag tcgatggaaa ttattcaggc caatggcgcg acgctggctg gcgtattgat
    30481 ttcgcttgat cgtcaggaac gcgggcgcgg cgagatttcg gcgattcagg aagttgagcg
    30541 tgattacaac tgcaaggtga tctctatcat caccctgaaa gacctgattg cttacctgga
    30601 agagaagcca gaaatggcgg aacatctggc ggcggttaag gcctatcgcg aagagtttgg
    30661 cgtttaaaga aactcgccgg atgaaaagtc atccggcgtc atattactgc aactgtgcgg
    30721 caattagcgg ccagcgggcg tcaaaatcat ccgtcgggcg gtatttaaac tcgctgcgga
    30781 caaagcgtga cagcatacct tcacagaagg ccagcaactg gcttgccagc agggtttcat
    30841 cggtggtgta accttcaccc tcacgcattc tcttttcacg caatacctga cgcaactgcg
    30901 cttcaatacg ctcgaacagc tggttgatgc gcccttgcag acgatcctgt tcgaacatta
    30961 gcgcatgacc agtgaggatg cgagtcaggc caggattacg ctcaccaaaa ccgagaagca
    31021 gcaacacaat cagacgcagg cgcgctgtgg tgtccttctc atctttcaga atcagattga
    31081 tgcgagtaat caggctatct tcgataaact caatcaggct atcgaacatg cgggtcttac
    31141 tggggaagtg gcgatacagt gccgcttcgg aaacgccgac agaggcggcc agttttgccg
    31201 tcgtgatacg ttggcttcca tcgctggatt ccagcatcag cgccagagac tgaagtattt
    31261 cctcgcgacg gttccttttc gcagtttgtt tttctgccat gttacaaaat acccctgaaa
    31321 ataagcactt gccaggcggc acccacgcta tgaccgcaaa cgaaatgttt gcggctatgt
    31381 tatgacgtta ttcggatgcg tatgtgttac tgacgaccag agtgaccaaa gccgccttca
    31441 ccgcggtcgg tggcgtcgaa atcttccacc agattaaatt cagcctgtac taccggaaca
    31501 aaaatcatct gggcgatgcg ttcgccaggt tgaatggtga agctgtcctg accacggttc
    31561 cacacggaaa tcatcaactg gccctgatag tcagaatcga tcaatcctac caggttacca
    31621 agcacgatac cgtgcttatg tcccaatccg gagcgcggta gcatcattgc cgccagtgaa
    31681 ggatcggcaa tatgaatcgc cagcccggtc ggaaccagcg tagtgtcacc cggagccagt
    31741 tctacggcgt cgtcgagaca ggcacgcagg tcaagtccgg cagagccaga ggtggcataa
    31801 gtcgggagcg gaaattcctt cccaacgcgc gggtccagaa tcttaacgtc gatttttttc
    31861 atcataacgg gtcacgatct cgtcgagtaa taattggcca aggagctctt tgcgctcaag
    31921 cggtaagact ttatctccgt cctgccagaa aaggtgtaat gcgttgttgt cgctgttaaa
    31981 tccttgagtt ggctgggaaa catcgttcgc gcagatcaga tcaaggtttt tacggatacg
    32041 tttttgccgg gcgtattctt ccacattatt tgtttcggcg gcaaatccaa cgacgtaggg
    32101 gcgatggtct tttagtgcgg caacgcctgc gacgatatcg gggtttttaa ccatttttat
    32161 tgttaattca tcaccctgcg tggcctgctt tttgattttt tctggggcca cggtagctgc
    32221 gcgataatcc gccacggcgg cgcaaccgat aaaaatattt tgctgctgta cagatgcatt
    32281 cacggcggct tccatttcca gcgcggtcat cacatcaaca cgtttaacaa acggtggcgt
    32341 cggtagtgaa accggacctg ataccagcgt gacgttcgcg ccacgacggg cagcggcggc
    32401 ggcgatagca aaacccatct tgcctgagct gtgattagag atataacgca ccggatcgag
    32461 tggttcacgc gtcgggccgg cggtaatcat aatgttcaga tgtttcaggt cgttgacggg
    32521 cgaaaaatgc gctaccgcca tatcaacaat ggttaacgga tcgagcattc gcccagggcc
    32581 gatatcacca caagcctgac tgccactgtc tggcccccaa atgagcaaac cacgggaagc
    32641 aagcacctct aaattatgct gcgtggcagc ggcacggtac atctgctggt tcatggcggg
    32701 gagcacggct acaggcgcag gtgtggccag acaaatcgtc gataccagat cattcgccat
    32761 tccggcagca acacgggcaa tcaaatctgc cgtggcaggg gcgagaatca ctaaatcagc
    32821 ccatttaccc agctcaatat ggcccatagc ggcttcggct gctgggtcca gcagactgtc
    32881 ggaaacggga taaccagaaa ccgcctgcaa gctaagtggg gtgataaagg cttttgccgc
    32941 ttcggtcatg gctacgcgga cgtcggcccc gcgctcgcgc aaacgacgca ccagttcagg
    33001 ggttttatag gcagcaatac cgccgctgac gccgagaacg atttttttac cggccaggct
    33061 catgatgatt tttcctgttg ggtgacacca gaagttggcg attttatcac aatacttttg
    33121 ttgtcgtgcc ttcacctgag aatcactttg cgaggcgctt tccaggattg aaaactggcc
    33181 gtcgatttaa cggaacggct atgacaggat gcgagcacca caaaggaggt gaaggtgaaa
    33241 aacaattcac agctgttgat gccgcgcgaa aaaatgctga agtttggtat tagcgcctta
    33301 acggatgtcg agctgctggc gctatttctg cgtaccggaa cgcgcggtaa agatgtatta
    33361 accctggcaa aagagatgct ggagaatttc ggctctcttt atggcttgtt aacctctgaa
    33421 tatgaacaat ttagtggtgt tcatggaatt ggcgtggcga aatttgccca gttaaagggg
    33481 attgccgaac tggcgcggcg ttactacaac gtgcggatgc gtgaagaaag ccctttactc
    33541 agcccggaga tgacgcggga atttttacaa agccagctca cgggtgagga gcgggagatc
    33601 tttatggtga tctttctcga ctcacaacac agggttataa cgcatagccg tcttttttcc
    33661 ggcacgctaa accatgttga agtccatcct cgggaaatta tccgcgaagc gataaaaata
    33721 aatgcctcgg cgctgatcct tgcacataat cacccttcgg gttgtgctga acccagtaaa
    33781 gcggataaac tcattactga acggataata aagagttgtc agttcatgga tttacgcgtg
    33841 ctcgaccata tcgtgattgg gcgtggagag tatgtttctt ttgccgaacg cggctggatt
    33901 taacccgcta tgcgcgatcc ttcgggatct ttgtctgttc gggacttgag cacatcgctg
    33961 agtcagcgta tactacgcca cctttgagaa tctcgggttt ggcatttggg cctggcaatc
    34021 gagagttcac atagaactgc gatgaccggg ctgtaaagcc tgacgaggcg ccaatacccc
    34081 atacgaagct cgagctaatt tgatttttgg agaatagaca tgtcccgagt ctgccaagtt
    34141 actggcaagc gtccggtgac cggtaacaac cgttcccacg cactgaacgc gactaaacgc
    34201 cgtttcctgc cgaacctgca ctctcaccgt ttctgggttg agagcgagaa gcgttttgtc
    34261 accctgcgcg tatctgctaa aggtatgcgt gtaatcgata aaaaaggcat cgatacagtt
    34321 ctggctgaac tgcgtgcccg tggcgaaaag tactaagtac ttagaggaaa taaatcatgg
    34381 ctaaaggtat tcgtgagaaa atcaagctgg tttcttctgc tggtactggt cacttctata
    34441 ccactacgaa gaacaaacgt actaagccgg aaaaactgga actgaaaaaa ttcgatccag
    34501 ttgttcgcca gcacgtgatc tacaaagaag cgaaaatcaa ataatttttg ctttgatgta
    34561 acgaaaaacc tcgccccggc ggggtttttt gttatctgcc tgcccccata ttgactgcat
    34621 ctgttcattc ctggagatgc tatgcctgaa ttacccgaag ttgaaaccag ccgtcgcggc
    34681 atagaaccgc atctcgttgg tgcaaccatt cttcatgcgg tggtgcgcaa cggacgcttg
    34741 cgctggccgg tttcagaaga gatctaccgt ttaagcgacc aaccagtgct tagcgtgcag
    34801 cggcgggcta aatatctgct gctggagctg cctgagggct ggattatcat tcatttgggg
    34861 atgtctggca gcctgcgcat ccttccagaa gaacttcccc ctgaaaagca tgaccatgtt
    34921 gatttggtga tgagcaacgg taaagtgctg cgctacaccg atccgcgccg ctttggtgcc
    34981 tggctgtgga ccaaagagct ggaagggcac aacgtgctgg cccatcttgg accggagccg
    35041 cttagcgacg atttcaacgg tgagtacctg catcagaagt gcgagaagaa aaaaacggcg
    35101 attaaaccat ggctgatgga taacaagctg gtggtaggcg tagggaatat ctatgccagc
    35161 gaatctctgt ttgcggcggg gatccatccg gatcggctgg cgtcatcact gtcgctggca
    35221 gagtgtgaat tgttagctcg ggtgattaaa gcggtgttgc tgcgttcgat tgagcagggc
    35281 ggtacaacgc tgaaagattt tctgcaaagt gatggtaaac cgggctattt cgctcaggaa
    35341 ttgcaggttt acgggcgaaa aggtgagtcg tgtcgggtgt gtggtacgcc gattgtggcg
    35401 actaaacatg cgcagcgggc aacgttttat tgtcggcagt gccagaagta attgatgcgc
    35461 gccggatggc ataccatccg gcataaactc tacgctaact tcgccatcag cgcctgatgg
    35521 acattctccg gcaggaaatg ggtgacatcg ccctgatggc gcgccacctc tttcaccaac
    35581 gatgaagaga taaacgacca ctctttcgac ggcatcagaa acacactttc cagttccggc
    35641 attaagtggc gattcatatg cgccagctgc atttcatatt caaaatccgc caccgcacgc
    35701 aggccacgaa tcagcaccgt agcgtgttga ttacgggcga agttcgccat caaatcacta
    35761 aacccgacca cttccacgtt ccccagatgc gcggttgcct gctgtgccag cgccacacgc
    35821 tcttccagag taaacatcgg ttttttactg ggactggcgg cgatcgccag aataacgtga
    35881 tcgaacattt gcgtggcgcg cgtcacgata tcgatatgac cattggtaat gggatcgaaa
    35941 gtacccggat aaatcgcccg tttttgcata acaacctcaa tgcgttttcg gtggcaggta
    36001 aggttccagc agttgaagca gacgctgtag cgcgccctgg ttttgataca gtacttcaac
    36061 ggcatgacgg ccatagaaac tacggtaatc ggcgtcggtg agtaaagagg aaacctcttt
    36121 tgcaagcgac gtggcatcgg taacggtaat cagcccgctt gcctgctcca gccgcgcgca
    36181 aatgtcttta aagttaaaag tatgaggccc catcaatacc ggaatagcgt gtgcggcagc
    36241 ttccagcgga ttatgcccac cacgttcaac cagtgaaccg ccaacaaagg cgagatcggc
    36301 aatgccatac agtaacatca actcgcccat cgtatcgcca accacaacct gcgtgctggt
    36361 ggaggggact tcccctgaag agcgtgtgat atagcttagt ccagcctggc ggacaaggtt
    36421 aatcgcatcc gggaagcgtt ccggatgacg ggggaccagg atgagcaata aattcgggaa
    36481 ttgctgtaac aatgcctgat gtgcggcgat caccacactc tcttcgcctt cgtgagtgct
    36541 ggtggcaatc cataccgggc ggtgtggtgc ccactggcgg cgcagcgtca cggctttagc
    36601 agccaactgc ggcgttacag aaatatcgaa tttcaggcta ccggtaacgg ttacctggtt
    36661 attttttgcg cccagcgcca caaaacgtgc accatcttct tcattttgcg cagcaatcag
    36721 cgtaatacga cgcagcaagc gacggacgaa tttacccagt ttggcataac ctgcggccga
    36781 gcgggcagag agtcgcgcgt tagcgatcac cagcggaatt ttacgtttat gtagcgccgc
    36841 aatcaggtta ggccatagtt cggtttccat aatcaacact agtttagggt cgactttatt
    36901 caggaaacga ttaagtgcat cgggcaggtc atatggcaga taaacgtgct gaacatcctt
    36961 cccgaaagcc gattgtacgc gctccgaacc ggttggcgtc atggttgtaa cggtaatcgg
    37021 taaatcagga taacgatggc gcagcgcgcg caccaacggg atcgccgcca gagtttcacc
    37081 gacggagacg gagtgcagca tgatgccgcc tggttttagc ggatggcggt aaaaaccgta
    37141 acgttcaccc cagcgttttc gataggccgg agccttacgt ccgcgcaccc agagccgtat
    37201 ccagatcagc ggctgaataa ggtagagaag ggcggtgtaa agcaattcga gcatagtaaa
    37261 tagctgactt atggatgtgc tggggattct atgtatttag ctgtggcttt accattactt
    37321 ttcccgtttt tgacttaaat agcaatttac ccgccaattc ttgctgaaca tctatacttt
    37381 ttttgtattt ttatgcgatt cattgatttc tgcagtacgt gcaggaagga atcgcagagc
    37441 aacagtgaaa gaatatggtt gctaatgatt ttatttgatt gatttcttta ttgaaaatca
    37501 acaatgtgct taaaaatcat tcgggtcgga ttgccgcgaa agaagtgata cactagcgcg
    37561 tcaatatcag tgcggtatca gtattcaggt agctgttgag cctggggcgg tagcgtgctt
    37621 tttctgcttc accttaatcg gataatctca acaaaaagag ttacttgtgg ataagccatt
    37681 tcgaagaatt ttgctcatta agatgcgttt tcatggggat atgttattaa ctactcccgt
    37741 cattagttcg ctgaaaaaaa attaccctga cgcaaaaatc gatgtgctgc tttatcagga
    37801 caccatcccg atcctgtctg aaaatccaga gattaacgcg ctctacggca taaaaaataa
    37861 aaaagcaaaa gcctcagaaa aaattgccaa ctttttccat ctcatcaagg tattacgtgc
    37921 caataagtat gaccttatcg ttaatcttac cgatcaatgg atggttgcta tactggtgcg
    37981 cttattaaat gcccgtgtga aaatttccca ggattatcat catcggcagt ctgctttttg
    38041 gcgtaaaagt ttcacccatt tggtgccgtt gcagggtgga aatgtggtgg aaagtaactt
    38101 atccgtgctg acaccactgg gagttgattc gttggtgaag cagacaacca tgagttaccc
    38161 gcctgcaagc tggaaacgta tgcgtcgcga acttgatcac gctggtgttg gacaaaatta
    38221 tgtggttatc caacctacgg cgcggcaaat cttcaaatgc tgggacaacg ccaagttttc
    38281 cgctgtgatt gatgccttac atgctcgtgg ttatgaagtc gttctgacgt ccggcccaga
    38341 taaagacgat ctggcctgcg tcaatgaaat tgcgcaggga tgccagacgc caccagtaac
    38401 ggcgctggcc ggaaaggtga ccttcccgga acttggtgcg ttaatcgatc atgcgcagct
    38461 gtttattggc gttgactccg caccggcgca tattgccgct gcagttaata cgccgctgat
    38521 atcgctgttt ggtgcgacag accatatttt ctggcgtccc tggtcaaata acatgattca
    38581 attctgggcg ggagattacc gggaaatgcc aacgcgcgat cagcgtgacc gaaatgagat
    38641 gtatctttcg gttattccgg cggcagatgt cattgctgct gtcgataaat tactgccctc
    38701 ctccacgaca ggtacgtcgt tatgatcgtt gctttttgtt tatataaata ttttcccttt
    38761 ggcggtttgc agcgcgattt tatgcgtatt gctcagacag tcgccgcccg aggtcatcat
    38821 gttcgggttt atacccagtc gtgggaaggc gaatgccctg atgtatttga actgatcaaa
    38881 gtgccggtta aatcgcatac caatcacggg cgcaatgcgg agtattttgc ctgggtgcaa
    38941 aaacatttac gcgaacatcc cgtcgataaa gtcgttggat tcaacaaaat gccggggctg
    39001 gacgtttatt atgccgctga tgtttgttat gccgagaaag tagcgcagga aaaaggcttt
    39061 ttctatcgcc tgacgtcacg ttatcgccat tatgccgcct ttgagcgggc aaccttcgaa
    39121 cagggcaagc cgacacagct gctgatgctg acagataagc aaatcgccga tttccagaaa
    39181 cattatcaga ctgaagcgga gcgttttcat attctgccac cggggattta tcctgatcgt
    39241 aaatatagcc agcagccagc taatagccgt gaaatcttcc gtaagaagaa tggaataacc
    39301 gaacaacaat atttattgtt gcaggtcggt tcagacttca cgcgtaaagg tgtcgatcgt
    39361 tccattgaag cacttgcttc gttaccggat tcgctgcgcc acaacacatt gctatatgtt
    39421 gttgggcagg ataaaccgcg aaaatttgag gcgctggcag aaaaacgcgg cgtgcgcagt
    39481 aatgttcact tcttctcggg gcgcaacgat gtctcggaat taatggcggc ggcggattta
    39541 ttactgcatc ctgcctacca ggaagcggcg ggaattgtgc tgctggaagc gattactgca
    39601 ggattaccgg tactaacaac tgccgtttgt ggctatgcgc attatattgt cgacgccaat
    39661 tgcggcgagg ctattgctga gccattccgc caggaaacat tgaatgagat tttacgcaaa
    39721 gcgttaacgc aattttcatt gcgccaggcc tgggcggaaa atgcgcgaca ttatgctgat
    39781 acacaagatt tatacagtct gccagagaaa gcggcggata tcataacggg tggtctggat
    39841 ggttgaactt aaagagccgt ttgccacgtt atggcgcgga aaagatcctt ttgaggaagt
    39901 taaaactttg cagggtgagg tatttcgtga actggaaact cgccgtactc tgcgctttga
    39961 aatggcgggc aaaagctatt ttctcaaatg gcatcgcggc acgaccctga aagagataat
    40021 caaaaattta ctctcattgc ggatgccagt attaggcgca gaccgcgaat ggaatgcgat
    40081 tcatcgactg cgggatgtcg gcgttgatac tatgtatggg gtggcattcg gcgaaaaagg
    40141 cattaatccg ctcaccagaa cctcgtttat tataaccgaa gatctgacac caaccatcag
    40201 tctggaagat tactgtgctg actgggcgac taaccctcca gatgttcgcg taaagcgtat
    40261 gcttattaag cgtgtcgcga cgatggtgcg cgatatgcat gctgcgggca ttaaccaccg
    40321 tgactgttat atctgtcatt tcctgctgca cttgcctttt tccggtaaga aagaggagtt
    40381 aaaaatttcg gtaattgatc tgcaccgggc gcagcttcgc acgcgcgttc cacgtcgttg
    40441 gcgcgataaa gatcttatag ggctttattt ttcttcgatg aatattggcc tgactcagcg
    40501 ggatatctgg cggtttatga aagtgtattt tgtcgcctcg cttaaagaca ttctcaagca
    40561 ggaacaagga ctgctgtcgc aagcagaagc aaaagccaca aaaatcaggg aaagaacgat
    40621 tcgaaaatcg ttgtaatttc cggagagaaa tatgagtgcc cactatttta atccacaaga
    40681 gatgatcaat aagacaatca tcttcgatga aaggccagcg gcgtcagtgg catcatcatt
    40741 ccatgttgct tatggcattg ataaaaactt tctttttggt tgtggtgttt caatcacgtc
    40801 agttttgtta cataacaacg acgtaagttt tgttttccac gtttttattg atgatatccc
    40861 tgaagccgat atccagcgtt tagcccaatt ggcgaaaagc tatcgtacct gtatccagat
    40921 ccatctagta aattgtgaac ggcttaaggc attaccgacg accaaaaatt ggtctattgc
    40981 catgtatttc cgttttgtaa ttgcagatta ctttattgat caacaagata agatccttta
    41041 cctggatgct gatatcgcct gtcagggaaa cttaaagccg ctgataacaa tggatcttgc
    41101 caataacgtt gctgctgttg ttactgaacg cgatgctaac tggtggtcgt tacggggtca
    41161 aagtctgcag tgtaatgaac ttgaaaaggg ttactttaat tcaggtgtcc tgttaattaa
    41221 tacactagcg tgggcgcagg agtccgtttc tgctaaagcg atgtcgatgc ttgctgataa
    41281 agccatcgtt tcccgtttaa cctatatgga tcaagatatc cttaatctta tcctgttagg
    41341 gaaagttaaa ctcattgaag ctaaatacaa tacgcaattt agtttaaatt atgaattaaa
    41401 aaaatcattt gtttgtccaa ttaatgatga aaccgtatta attcattatg tcggcccgac
    41461 aaaaccctgg cattactggg ccggttatcc aagtgcgcaa ccttttatca aagccaaaga
    41521 agcatcgccc tggaaaaatg aaccgttaat gcggccagtt aactcaaact atgctcgtta
    41581 ttgcgctaag cataatttt
//
LOCUS       DAZGHF010000025        40956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGHF010000025 DAZGHF010000000
VERSION     DAZGHF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.025"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(26..716)
                     /locus_tag="ACHK3A_001476"
                     /pseudo
     CDS             complement(26..716)
                     /locus_tag="ACHK3A_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
     gene            complement(805..1638)
                     /gene="fdhD"
                     /locus_tag="ACHK3A_001477"
     CDS             complement(805..1638)
                     /gene="fdhD"
                     /locus_tag="ACHK3A_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO8739582.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRHDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1832..4882
                     /gene="fdnG"
                     /locus_tag="ACHK3A_001478"
     CDS             1832..4882
                     /gene="fdnG"
                     /locus_tag="ACHK3A_001478"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2417..2419,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO8739583.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKVYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4895..5797
                     /gene="fdoH"
                     /locus_tag="ACHK3A_001479"
     CDS             4895..5797
                     /gene="fdoH"
                     /locus_tag="ACHK3A_001479"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO8739584.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5794..6429
                     /gene="fdoI"
                     /locus_tag="ACHK3A_001480"
     CDS             5794..6429
                     /gene="fdoI"
                     /locus_tag="ACHK3A_001480"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO8739585.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            6426..7355
                     /gene="fdhE"
                     /locus_tag="ACHK3A_001481"
     CDS             6426..7355
                     /gene="fdhE"
                     /locus_tag="ACHK3A_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO8739586.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(7570..7788)
                     /gene="yiiE"
                     /locus_tag="ACHK3A_001482"
     CDS             complement(7570..7788)
                     /gene="yiiE"
                     /locus_tag="ACHK3A_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO8739587.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQRGETVVVDDQCKEHKE"
     gene            complement(8393..8683)
                     /gene="nadS"
                     /locus_tag="ACHK3A_001483"
     CDS             complement(8393..8683)
                     /gene="nadS"
                     /locus_tag="ACHK3A_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO8739588.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(8684..8995)
                     /locus_tag="ACHK3A_001484"
     CDS             complement(8684..8995)
                     /locus_tag="ACHK3A_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8739589.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            9224..10132
                     /locus_tag="ACHK3A_001485"
     CDS             9224..10132
                     /locus_tag="ACHK3A_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8739590.1"
                     /translation="MAQEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEAMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(10196..11137)
                     /gene="fabY"
                     /locus_tag="ACHK3A_001486"
                     /gene_synonym="yiiD"
     CDS             complement(10196..11137)
                     /gene="fabY"
                     /locus_tag="ACHK3A_001486"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO8739591.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(11182..11619)
                     /gene="dtd"
                     /locus_tag="ACHK3A_001487"
     CDS             complement(11182..11619)
                     /gene="dtd"
                     /locus_tag="ACHK3A_001487"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO8739592.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDCA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11616..12488)
                     /gene="yihY"
                     /locus_tag="ACHK3A_001488"
     CDS             complement(11616..12488)
                     /gene="yihY"
                     /locus_tag="ACHK3A_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO8739593.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12482..13081)
                     /gene="yihX"
                     /locus_tag="ACHK3A_001489"
     CDS             complement(12482..13081)
                     /gene="yihX"
                     /locus_tag="ACHK3A_001489"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO8739594.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13222..14016)
                     /locus_tag="ACHK3A_001490"
     CDS             complement(13222..14016)
                     /locus_tag="ACHK3A_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO8739595.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASFPASAGWHPWFAKKLTPQNTESLQVLF
                     DADWQEEAGSDELPTGNRISPQVGPWDDCFGFYDGVKVKLLWPGKLAMTMTSSANSLV
                     VFDKQPDATCINPLTQAPNAINLTPEFVTPDKPLVIETRWQFTPES"
     gene            complement(14006..15355)
                     /locus_tag="ACHK3A_001491"
     CDS             complement(14006..15355)
                     /locus_tag="ACHK3A_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8739596.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(15454..16887)
                     /locus_tag="ACHK3A_001492"
     CDS             complement(15454..16887)
                     /locus_tag="ACHK3A_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8739597.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(16957..17967)
                     /locus_tag="ACHK3A_001493"
     CDS             complement(16957..17967)
                     /locus_tag="ACHK3A_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO8739598.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(18000..18887)
                     /locus_tag="ACHK3A_001494"
     CDS             complement(18000..18887)
                     /locus_tag="ACHK3A_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO8739599.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(18912..19790)
                     /gene="yihU"
                     /locus_tag="ACHK3A_001495"
     CDS             complement(18912..19790)
                     /gene="yihU"
                     /locus_tag="ACHK3A_001495"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO8739600.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            19963..20859
                     /locus_tag="ACHK3A_001496"
     CDS             19963..20859
                     /locus_tag="ACHK3A_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO8739601.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            20899..21687
                     /locus_tag="ACHK3A_001497"
     CDS             20899..21687
                     /locus_tag="ACHK3A_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO8739602.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(21747..23012)
                     /gene="yihN"
                     /locus_tag="ACHK3A_001498"
     CDS             complement(21747..23012)
                     /gene="yihN"
                     /locus_tag="ACHK3A_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8739603.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(23114..24094)
                     /gene="yihM"
                     /locus_tag="ACHK3A_001499"
     CDS             complement(23114..24094)
                     /gene="yihM"
                     /locus_tag="ACHK3A_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO8739604.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(24102..24812)
                     /gene="yihL"
                     /locus_tag="ACHK3A_001500"
     CDS             complement(24102..24812)
                     /gene="yihL"
                     /locus_tag="ACHK3A_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8739605.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTRDIPETLVPLITVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(25029..26852)
                     /gene="typA"
                     /locus_tag="ACHK3A_001501"
     CDS             complement(25029..26852)
                     /gene="typA"
                     /locus_tag="ACHK3A_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO8739606.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRSPKDD"
     gene            27224..28633
                     /gene="glnA"
                     /locus_tag="ACHK3A_001502"
     CDS             27224..28633
                     /gene="glnA"
                     /locus_tag="ACHK3A_001502"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO8739607.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLA
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            28807..29856
                     /gene="glnL"
                     /locus_tag="ACHK3A_001503"
     CDS             28807..29856
                     /gene="glnL"
                     /locus_tag="ACHK3A_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO8739608.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            29868..31277
                     /gene="glnG"
                     /locus_tag="ACHK3A_001504"
     CDS             29868..31277
                     /gene="glnG"
                     /locus_tag="ACHK3A_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO8739609.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            31389..31499
                     /gene="yshB"
                     /locus_tag="ACHK3A_001505"
     CDS             31389..31499
                     /gene="yshB"
                     /locus_tag="ACHK3A_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO8739610.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(31689..33062)
                     /gene="hemN"
                     /locus_tag="ACHK3A_001506"
     CDS             complement(31689..33062)
                     /gene="hemN"
                     /locus_tag="ACHK3A_001506"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO8739611.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(33251..33760)
                     /gene="yihI"
                     /locus_tag="ACHK3A_001507"
     CDS             complement(33251..33760)
                     /gene="yihI"
                     /locus_tag="ACHK3A_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO8739612.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(33879..34045)
                     /locus_tag="ACHK3A_001508"
                     /pseudo
     CDS             complement(33879..34045)
                     /locus_tag="ACHK3A_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34342..34974
                     /gene="yihA"
                     /locus_tag="ACHK3A_001509"
     CDS             34342..34974
                     /gene="yihA"
                     /locus_tag="ACHK3A_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO8739613.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(35298..38084)
                     /gene="polA"
                     /locus_tag="ACHK3A_001510"
     CDS             complement(35298..38084)
                     /gene="polA"
                     /locus_tag="ACHK3A_001510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO8739614.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPVAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVVDEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            38448..39380
                     /gene="yihG"
                     /locus_tag="ACHK3A_001511"
     CDS             38448..39380
                     /gene="yihG"
                     /locus_tag="ACHK3A_001511"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO8739615.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCESLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(39421..40851)
                     /gene="yihF"
                     /locus_tag="ACHK3A_001512"
     CDS             complement(39421..40851)
                     /gene="yihF"
                     /locus_tag="ACHK3A_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFO8739616.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMRGNVIPALAAAKVKLVDNELTQPLFIAAKNKSPMEATLRFAFGGSFSTTLDVAP
                     AKYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDLVLELSPLNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQINIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFVIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLIFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT        10256 a        10536 c         9931 g        10233 t
ORIGIN      
        1 ttatccttca ctctgcttta tattgtcacg acttcgtgac atatccgcgc tcatcacaaa
       61 cccgctccgc gattacaatt gactgaaata tttcatcgac catttcgttg tactcgcata
      121 atgctctcgg gaaatgagtc ttcggccatc cagcttgagt ttgcgtcctg aagttgctct
      181 ttatcccagt cagagtacgg gactttatct ctgatgcggc gaacgtattt tttagcctcg
      241 agttgatact ggcctgccga attaataaac gagaaattca tgccgctttt atccgcttct
      301 ttggcctgcg ccaccagcgt ttccagttca gacactttct tcattgccgc ttctgcatca
      361 aacttatctt gcgatatcag gttattaatt tgtttcgctg aaatcatgac tgccagagag
      421 tagtagtgga atgttttccc ttctctttct tcaatatttt tcaattccgt aagctggcgc
      481 ttgtcattaa tttcctgaat cgccgcatga taagattctg ctacaggttc aaaggcttcc
      541 agatttttta agaatgtctg gtgcagcgtt ttccctttcg caaacgcatc gtctttataa
      601 ttttcctgga tgtaatattt atccatttcg ttaatggtat tacccaacgc tacggcagca
      661 tcaatataac tcaccgccac gccatcaata ggttgcaacg caggcgttaa cgacactaca
      721 cgcgttttgt ttataaataa aataataaaa ttcacgaaat gaataagtcc tgcaatttaa
      781 tatatatcgc aggacttatt ttaattaatt gctcaaacgc tgcggatggg tataaaccgt
      841 tgcccgaccc ggtttacaaa agcccaccag cgtcaaatta cagcgttcag ccacttctac
      901 agcaagtgtg gtcgcggcag acaccgcaaa caaaatctct acgccgcaca tcgccgactt
      961 ttgcaccatt tcataactgg cacggctgga aaccagtacc gcaccttgct gccagctttc
     1021 cccttcttgt gaccggcggc ctaacagttt gtccagcgcc acatggcgac ccacgtcttc
     1081 atgcccgccg accagttcgc cagatggcaa catccaggcg gcggcgtgag tacaaccggt
     1141 cagttgcccc actggctgaa aatcattgag atgacgtaat gcatcatcca gtttgttgag
     1201 atcaaacgtc tgggtgaacg gtagcggctg caccggtttt ccgatgtcat taagttgctc
     1261 cacgccgcac acgccacatc ccgtacgtcc cgccagtgcc cggcgacgct ccttcaaccc
     1321 cataaagcgg cggctggaaa gctcaatttg aacttcaaga ccattacagg aaggaacgac
     1381 atccatgccg aagatatcgc gcggactttc gattatcccc tcggaaagcg aaaaaccgag
     1441 cgcaaagtac tcaaggtctt tgggcgacgc catcatcacc acatgcgaaa taccgttgta
     1501 gaccagcgca acgggaactt cttccgccac ctcatctaac cgtgggtgtt gtaaatcatc
     1561 atgccgccat aactcaattt gacgaacacc tgtgatattt gtcacatttt cgatttcttt
     1621 ttgctgtgtt tttttcactt tctcttaacc agataagaac acacacacca acattatggt
     1681 attctgttac aaacccttcc tggatggagg gaaattgagc caattctgga cctttgcggc
     1741 cccttccgca aagaaaaata actcccactc cctgcacacg cagcaagcga atgtaaatgg
     1801 gacgtgacaa tgtcgaaaca aggagcaatc catgcaggtc agcagaaggc agttctttaa
     1861 gatctgcgct ggcggtatgg caggcaccac ggcagcggca ctgggttttg cacccagcgt
     1921 agcactcgcg gaaacccggc agtataaact gctgcgcacc cgcgaaaccc gtaatacctg
     1981 cacctattgt tccgtaggct gtgggctgtt gatgtacagc ctcggtgacg gagcaaaaaa
     2041 cgccaaagca tctatcttcc atatcgaagg tgacccggat cacccggtca accgcggtgc
     2101 actttgtccg aaaggcgctg gcctggtgga tttcatccac tccgaaagcc gtctgaagtt
     2161 tccggaatac cgtgcgccag gttctgataa atggcagcaa atcagttggg aagaggcgtt
     2221 tgatcgcatc gccaaactga tgaaagaaga ccgcgatgct aactacattg cgcaaaacgc
     2281 cgaaggcgtg accgttaacc gctggctctc caccgggatg ctgtgtgctt ccgcgtcgag
     2341 taacgaaacc ggctatttaa cgcaaaaatt ctcccgcgcg ctgggtatgc tcgcggtcga
     2401 caaccaggcg cgtgtctgac acggaccaac ggtagcaagt cttgctccaa catttggtcg
     2461 cggtgcgatg accaaccact gggtcgacat caagaacgcc aacctcgtcg tggtgatggg
     2521 cggtaacgcc gctgaagctc acccggtcgg gttccgctgg gcgatggaag ccaaaattca
     2581 caatggcgcg aagctgattg tgatcgatcc tcgctttacg cgtacggcgg cggtagctga
     2641 ctactatgcc cctattcgtt ccggtactga cattgctttc ctgtcaggcg tattgctgta
     2701 cctgctgaac aatgaaaaat tcaaccgcga atacaccgaa gcctatacca acgccagcct
     2761 gatcgtgcgt gaggattacg gctttgaaga tggcctgttc accggctatg acgcggaaaa
     2821 acgtaagtac gataaatcct cctggactta cgaactggac gaaaacggct tcgccaaacg
     2881 cgatactacc ctgcaacatc cacgttgcgt gtggaacttg ctgaaacagc acgtttcccg
     2941 ctacacgcca gatgtggttg aaaacatctg tggtacgcca aaagacgcat tcctgaaagt
     3001 ctgcgaatac atcgctgaga ccagtgctca cgataaaact gcctcgttcc tttacgcgct
     3061 cggctggacg caacactcca ttggtgcgca aaacattcgt acgatggcga tgatccagtt
     3121 gctgctcggc aatatgggga tggcaggcgg cggcgttaac gccctgcgcg gtcactccaa
     3181 tattcagggg ctgacggacc tggggctgct gtcgcagagc ctgccgggtt acatgacgct
     3241 gccgagcgag aagcagaccg atctgcaaac ctaccttacc gccaacacgc caaaaccgct
     3301 gctggaaggc caggtaaact actggggcaa ctacccgaaa ttcttcgtct ctatgatgaa
     3361 ggccttcttt ggtgataaag cgacggcgga aaatagctgg ggctttgatt ggttgccgaa
     3421 gtgggataaa gtctacgacg tcctgcaata cttcgagatg atgaaagaag gcaaggtcaa
     3481 tggctatatc tgccagggct ttaaccctgt tgcctcattc ccgaacaaaa acaaagtgat
     3541 cggttgtctg tcgaaactga agttcctcgt caccatcgac ccgctgaaca ctgaaacctc
     3601 taacttctgg cagaaccacg gtgagctgaa cgaagttgac tcgtcgaaga tccagaccga
     3661 agtgttccgt ctgccatcga cttgcttcgc agaagagaac ggttcaatcg ttaactccgg
     3721 tcgctggtta cagtggcact ggaaaggtgc ggacgcccca gggattgcgc tgactgatgg
     3781 cgagatcctc tccggtatct tcctgcgctt gcgcaagatg tatgcagaac agggtggcgc
     3841 aaacccggac caggtgctga acatgacctg gaactacgcc attccgcatg agccatcttc
     3901 agaagaagtg gcgatggaga gcaacggtaa ggcgctggcc gatattaccg atccggcaac
     3961 cggtgcggtt atcgtcaaga aaggccaaca acttagctcg ttcgctcaac tgcgcgatga
     4021 cggtacaacc tcctgtggct gctggatttt cgccggtagc tggacgccgg aaggcaacca
     4081 gatggcgcgt cgtgataacg ccgatccgtc tggcctcggt aacacgctgg gctgggcatg
     4141 ggcatggccg cttaaccgcc gcattctgta taaccgcgcc tccgcagatc cgcagggtaa
     4201 cccatgggat ccgaagcgtc agttgctgaa atgggacggc actaagtgga ccggctggga
     4261 tattccggac tacagcgcag cgcctccggg cagcggcgta gggccgttta tcatgcagca
     4321 ggaaggcatg ggacgtctgt ttgccctcga taagatggcg gaaggtccgt tcccggaaca
     4381 ctacgagccg tttgaaacgc cgctgggaac taacccgctg catccaaacg ttatctcgaa
     4441 cccggctgcg cgtatcttta aagacgacgc cgaagcattg ggtaaagccg ataagttccc
     4501 gtatgtcgga accacctatc gtctgaccga gcacttccac tactggacca aacacgcgct
     4561 gttgaatgcg attttgcaac cagagcagtt tgtggaaatc ggggagtcgc tggcgaataa
     4621 acttggcatt gcccagggcg atactgtgaa agtctcctcc aaccgtggct atatcaaagc
     4681 caaagcggta gtgaccaaac gtattcgcac gctgaaagca aacggcaaag atatcgatac
     4741 catcggtatt ccgattcact ggggctatga aggtgttgcg aaaaaaggct ttattgccaa
     4801 tacgttgacg ccattcgtcg gtgatgcgaa cacgcagacg ccggagttta agtccttcct
     4861 tgtgaatgtg gaaaaggtgt aacggagacg acatatggct tatcaatcgc aagatatcat
     4921 tcgtcgttcc gcgactaacg gtctgacccc cgcgcctcag gcgcgggact tccaggaaga
     4981 agtggcgaaa ctcatcgacg ttaccacctg tatcggctgt aaagcctgtc aggtggcgtg
     5041 ttcagagtgg aacgacattc gcgataccgt cggcaataac attggggtgt acgacaaccc
     5101 caatgattta agtgccaaat cgtggacggt gatgcgattc tcggaagtgg agcagaacga
     5161 caaactggaa tggctgatcc gcaaagacgg ctgtatgcac tgttccgatc caggctgcct
     5221 gaaagcgtgc ccggcggaag gggcaattat tcagtatgcc aacggtatcg tcgacttcca
     5281 gtccgagcag tgcatcggct gcggttattg cattgcgggc tgtccgttcg acattccgcg
     5341 cctcaacccg gaagacaacc gcgtctacaa atgtacgttg tgcgttgacc gcgtggtggt
     5401 tgggcaagaa ccggcctgcg tgaagacctg cccaacgggc gcgattcact tcggtacgaa
     5461 agagtcgatg aaaacgctgg cgagcgagcg cgtggcggag ctgaaaaccc gtggttacga
     5521 caatgcgggt ttgtacgacc cggcaggcgt cggtggtaca catgttatgt acgtgctgca
     5581 ccatgctgac aagccaaatc tgtatcatgg cttgccggag aacccggaaa tcagcgaaac
     5641 cgtgaaattc tggaaaggca tctggaaacc gctcgcggct gttggctttg cggctacctt
     5701 tgcggccagt atcttccact acgtgggtgt cggtccgaac cgtgcggatg aggaagagaa
     5761 taatctgcac gaagagaaag acgaggagcg caaatgaaac gacgtgacac catcgtgcgc
     5821 tacaccgcgc cggaacgtat caaccactgg atcaccgcct tctgcttcat cctggcggcg
     5881 gtgagcgggc tgggcttttt gttcccgtcc ttcaactggt tgatgcaaat catgggcaca
     5941 ccgcagctgg cgcgcattct gcacccgttt gtcggcgtgg ttatgtttgc ctcgttcatc
     6001 atcatgtttt tccgttactg gcatcacaac ctaatcaatc gggatgatat cttttgggcg
     6061 aagaatattc gtaagatcgt cgtcaacgag gaagtaggtg acaccgggcg ttataacttc
     6121 ggtcagaaat gcgttttctg ggcggcgatt attttcctgg ttctgctgct ggtgagcggc
     6181 gtgattatct ggcgtcctta ttttgcacct gctttctcaa tcccggtgat ccgattcgcg
     6241 ttaatgctgc attcatttgc cgcagtggcg ttaattgtgg ttatcatggt gcatatctac
     6301 gccgcccttt gggtgaaagg cacgattacc gccatggtgg aaggatgggt taccagcgcc
     6361 tgggcgaaga aacatcaccc gcgctggtac cgtgaggtcc gcaagacaac ggaaaagaaa
     6421 gctgaatgag tattcgcata atcccgcaag atgagctggg ttcgagcgag aaacgtacgg
     6481 cggatatgat tccgccgtta ttgttccctc ggctcaagaa tttatacaac cgccgcgccg
     6541 agcgtctgcg cgagctggca gaaaataatc cgctgggtga ttacctgcgc tttgctgcgc
     6601 ttatcgccca cgcccaggaa gtggtgctgt acgaccatcc gctggagatg gatctgacca
     6661 cgcgcattaa agaagccagc gcacaaggca agcccccgct ggatattcac gttctgccgc
     6721 gtgataagca ctggcaaaag ctgctgatgg cgctgattgc tgagctgaaa cctgaaatga
     6781 gcggcccggc gctggcagtg attgagaatc tggagaaggc atcgactcag gagctggaag
     6841 atatggccag cgcactgttt gcctctgatt tctcgtccgt cagcagcgat aaagcgccgt
     6901 ttatctgggc tgcgctgtcg ctctactggg cgcagatggc taatctgatc cccggcaaag
     6961 cccgcgctga atacggcgaa caacgtcaat attgcccggt atgtggctct atgccggtgt
     7021 ccagcatggt gcaaattggc accactcagg gtctgcgtta cctgcattgc aacctgtgtg
     7081 aaaccgaatg gcacgtggtg cgcgtaaaat gcagcaactg cgagcagagc ggcaaactgc
     7141 attactggtc gctggatgac gaacaggccg cgattaaagc cgaaagctgc gatgactgtg
     7201 gcacttacct gaagattctt tatcaggaaa aagaaccgaa agttgaagcc gtggcagatg
     7261 acctcgcctc tctggtactg gacgcgcgaa tggagcaaga aggctacgcc cgcagttcca
     7321 tcaacccgtt cctgttcccg ggtgaagggg agtaatctct gacgttttcc gggtggcgct
     7381 ggtcgcccgg attacagctc atagggtgtg acgacacctg ctattcccgc aaaatctctc
     7441 gtattttgcg aggcgttttc cagatatgat gtaacagtta cagttattat aagttcaagt
     7501 aaaacagtct tgtattaata ccagaataag ggcatctggt ttttatattc aagaataata
     7561 aattttttgt cattccttat gctccttaca ctgatcatca acaactactg tctcacccct
     7621 ttgcctgcca ccctttgctt tacgcaccgg aaattgctgc cgctccagat attgctcaac
     7681 cgcttcgcgt aaaattgcag atcgtgaaac cttacgccag cccctaagct tgttcagccg
     7741 tttaattgcc tcttctgata aatgaagaaa aaccgtattc atcgccatta ccaatacctt
     7801 acatatatta ctcattaatg tatgtgcgaa aattacgccg aaaaatcatt taaaaaaagc
     7861 ggatttttgc atgctgtttt gtttttgcga gacgttttcc agaaataata agaacgttgc
     7921 agttgtttca tttgttaatc aaaaatggtt gggttatcgc ccaggatgag atatgggtgc
     7981 tataaatata ttatatacag ggatgtttat tttttgattg gatctttatt tgcataatct
     8041 gaatggtgta gccgaaaaat gtcagcatcc taacagcacc cggatttaag gtgaaattaa
     8101 tcatccatca cagaccggtc agtactgcta ccaaccggtc ttaacttgct catcgttata
     8161 attttgttgg taacagttcg ggaaacaagg aattccttcc ggaacacccc cggtatcatc
     8221 cgatagcatt gacgtcaatc atcggcgcga cgatgagttt ttttaatcgc atcatagggt
     8281 ggcaacggtc agcacgatta cgggtcatag caaaactcct gagcaggtgg cactacgccg
     8341 ctttcacagg agttctgaat gctggtgctt tctgtatggc ataaccatgc ctctaacggt
     8401 taagtgcttg cagcacatgt tgcggatcat tggcaatcgc tcgcagcaaa gcctttgcag
     8461 gcccggtcgg tgaacgtcgc ccctgctccc agttacgcaa cgtgtctacg cttacagaaa
     8521 taagatcggc aaatttagat tgtgacaacc ctgaagcgcg gcgtatttct ttgattttca
     8581 tagcatcaac ttcaaaagtc cgcgaaggct gtcgcgttcc ttcaataatt tcattcatct
     8641 gggccatgct gtgagtcaac cgctcaaaaa gtgccttatc catttaccac ctcgtattta
     8701 acaaccggag cattgctttt tcctggggag acagatcatc tttaataccc ttacgataaa
     8761 taagcagtaa tctgatctca taatgtttaa cctgatgaaa gtaaataacc ctgacaccag
     8821 cacgtttccc ctttcctcct gacacccatc gaacctttct taacccacca gtttcaggta
     8881 ttacctcacc atagtcagga ttcaaggcca ggaaaaactg aaaacgactg aattcatcgt
     8941 cggtcagcag tttttgaaca tcttcagtaa aaatttccgt ttcgatgaag agcataatta
     9001 tacgccattg gcttagagta acaatccttt ttgtacgtca ttgacgtacc cttgaaacca
     9061 ctagcttact gatagtgctc cagcaaaacc acgtctctgc cgggtttgtc tttacatttg
     9121 cgaggcgctt tccagatttt tcacgcccgt gaatttccct gtatccatcc agtaaaaacg
     9181 ggtgtataaa actgtacatt catacagcaa aatgagttct actatggcac aggaaaaagg
     9241 aattgcagag ttagtcgaag agtttatcgc tgcgggtcgc ccctcatcgc gtcaacaaag
     9301 tattgcgcag cgcagggagg gctatatcgc cagcgctgtc cttgcgggtg agacagaaac
     9361 gcgggtagat attcagacta tcgaacttga agcgatgacc ttacgaatag tgtcaccgct
     9421 gaatgccccg acattactcc cgacgataat ttattactac ggtggctgtt ttgttagcgg
     9481 cgggttcgca actcacgaca accagctgcg ccagttagcc tattacgggc aatgtcgggt
     9541 tattgccgtc cagtatcggc tggcaccgga acatactttc cccgccgccc atgatgatgc
     9601 gcaacgaggt gcagaattag tctggcaaca cgccgaacga ctcggcgttg ataaacaacg
     9661 gataactctt gccggtgaca gtgcgggcgg acatctggcg ctggtaactg ctttgcgatt
     9721 aaagcgtgct ggcgagtggc aaccggcaca actcatctta atttatccca tgcttgatgc
     9781 gactgcgcat ttcgaaagct acatacgcaa tggtcacgat tacatcatca cacgcgacac
     9841 tttactgagc ggctttgaga tgtacctgcc aggcattgaa cgcaggcatc ctgaagccag
     9901 ccctctttgg cgcgatgatt tcaatggctt gccacctgta catatcatta ccgctgaata
     9961 cgacccactt tgcgatgaag gtgaagcgtt gtatcaccgc atgacagggc aaggagtgca
    10021 atgcacctgc caacgctacc ttggcgttat ccacggattt tttcagttag gtggtatcag
    10081 tgaggctgcg cgatcggcgc tacgggatgt tgcctggaga gcgggaaggt aactccggac
    10141 cagggcccag aaaaggtttt cagattgaaa accttttcag tcaagtaaaa cgcccctact
    10201 cttcttcgtt cccgccctct tcatacgggc caaatggctt cgcgggcaga acgatatacg
    10261 tgccttcaaa cactgcaccc ggcgtctcgt cgccaaagat ttctacttgc atctgcaccc
    10321 gtgcttttcg tccgcgcgcc agacggtcga gatcgccgct taaggcacca aggtcggcta
    10381 ccgcatgagg tttgccgcta atcggcttgc tgtagcggat atgcgcatcc gccagaataa
    10441 tcgttccgcc gaggtggcgt tcgcgcagca tcagccagat aagcccccaa ccggtgagcg
    10501 ttgccagcga gaataaactc ccggcaaaca gcgtgtgatg cggattctga ttgcctgttt
    10561 ccggcatggt ggtgataaat ttttgcccgg tatattgctg aatgcgcacg cccatttttt
    10621 cactcagcgg gatgtgttca taccacgcct gttgcagctg cgcgcaccag tcgccgcgat
    10681 gcaaaatatc atccagagtg gcaacgggtt taatcatcaa aaaatggcga atcggcgtgg
    10741 tggttggcgt ggtgatttct ccctgattaa caaaccccag cttggcgaaa aactccaccg
    10801 cgtcttcgcg ggcgctacag gtcacgcgct taacaccttc ctgacgcgcc acagattcga
    10861 gggtcatcgc catcagcgtt cctaaccctt tgtcctgcac gtcgggatga acggccataa
    10921 agcgaatgga cgcttcattg tcggcattaa tatacagtcg gcctaccgcc accagattac
    10981 cctgctcgtc gacgaccatc tggtgatgcg ccatcgcatc ccacgcgtcg cgttccgaac
    11041 cttttggttg atgcaggggc ttacgcaaca tttcccagcg aaactgatag taacgctcta
    11101 attcttcttc tgtttgtgga acccgaaggt gatacatagc gatactctct cttgtttccc
    11161 gtgacaaccc tggaagctgg ctcatacctg caaccagaat gtcacggggc catcattgac
    11221 cagcgatacc tgcatatccg cagcgaagcg tcctgtttgc gtgttcatct cttgctgacg
    11281 gcagcgttcg acgaaatagt catataacgc ctctgcgcaa tccggtgatg cgcctttgga
    11341 gaaacttggg cgcatccccc gttcggtatc tgcggcaagg gtaaactggg aaaccaccag
    11401 tacactgccg cccgcctgtt gcacgttgag attcatcttg ccttcggcat cgctaaagat
    11461 gcggtagccg agcacacgct cgcacagacg gtttgctttc tgttcgtcgt catccttttc
    11521 gacacccaat aacaccaaaa gtcccgcgcc aatttcaccc gtcacttctc cctccacggt
    11581 gacgctggca cgggttacgc gttgaattaa tgcaatcatg gttcgtcgtc ttcttcttgt
    11641 tcagcagctt gttttaattt gcggtattcc ccgagagtga cagtaatttc cgcgccaagc
    11701 aagacgatac accatgtcca gtagacccaa acaaagagaa tggggatcac cgccagcaca
    11761 ccgtaaatga gctgatatga tgggaacatg gtgatataaa gcgcgaaacc tttctttcct
    11821 gcttcgaaca ggagcgcggc gacaaacgcg ccgacaatcg cgtcgcggtt aggtacacgg
    11881 atggtaggaa cgatgctgta cagcagccag aaagagatcc acgacaacag cagcggaaaa
    11941 atacgcaaca cattatcgat gacagtattg agatcgctcg cccagcgcag agagagcaga
    12001 taggagctga tcgccagact ggcccctgcc aacagcggcc ccagcgttaa aatcatccag
    12061 tacacggcaa acgagtaaat ttttggtcgc gctcgtttac tgcgccagat ggtattcaac
    12121 gcgctatcga tggagtacat caacaataac gccgtgacga tcagcccgca cgccccgacg
    12181 gcggtcattt tgttggaatt ggcgacaaac tgttcgatat atcgctggat aacatcgcca
    12241 gtagcaggca gaaagttggc aaaaataaag tgacgcaact gaatgctgac gtcggaaaac
    12301 atgggaaaag cggcgaaaag cgcaaaaaca acggcaacca gcggcactaa tgagagcaac
    12361 gacacatagg caaggttacc tgccagggtt gtcatgttgt cctcatcaat gcgttgccag
    12421 agtagtttta gccaggccca tagtggacgg gtacgatgcc tggctttgtc ctgaatggtt
    12481 tttagcataa caccttcgcg aaatagtccg ggatggtggt tttatctttc accagaatac
    12541 tagtaatgcc tagctgatta gctccttcta tattatcggc gttatcgtcg aaaaagaccg
    12601 tatcgtctgg tgaaaaacct tctgcctgca aaacatgctg ataaattcgt gcttcaggtt
    12661 tgcgcatacc caagtcttgc gacagataga tatggtcagc agcatcacgg atttccgggt
    12721 attcctccgg ccagaaggta gtatgcaggc ggttagtatt ggaaagcacc accacgcgat
    12781 gcccctgctc acgcagttta tgcatgatgg cgatcacttc cgggcgcagc gcaacaaata
    12841 ccgcctgcca accgtgggag aactgctcat agcttagcgg cagggccatc tcatggcaca
    12901 gtgcctctgc gaacgcttcg tcgctaattt ccccacgctc atgctgatga aacgcctcgc
    12961 ccatgtggaa actcttctta agcgtagcca gtggaacacg cgttaaatcg ctccaggccc
    13021 ccagcacacg gttaaagtcg atatcgacaa tcacattacc taaatcaaag atatagagca
    13081 tttttgcctc ctttcgcgcc acaagaaatt aactgtagcg ggaaaggatg ggtttggcta
    13141 tgtggcaata agggtaatct tgtgatgctg attgcgaaaa agcagagtaa tttcactggc
    13201 tttttcgcaa tagatgaatg gttaagattc aggtgtaaat tgccaacgag tttcgatgac
    13261 cagcggctta tctggtgtca caaattccgg cgttagattt attgcgttag gtgcctgagt
    13321 cagcgggtta atacaggtgg catcgggctg tttatcaaat accaccagac tgtttgcaga
    13381 agaggtcatg gtcatcgcga gtttaccagg ccacagtaac ttaaccttca ccccgtcata
    13441 aaaaccaaaa cagtcatccc agggaccgac ttgaggagag atacgatttc cagttggcag
    13501 ttcatcacta cccgcttctt cttgccagtc cgcatcaaaa agcacctgca acgattctgt
    13561 attttgcgga gtcagctttt tcgcaaacca cggatgccag cctgcggagg ccgggaatga
    13621 agccaggggc atacgtaaag tcagggaatc tgctgttttg tcgatgattt cccacgttga
    13681 gtaacaagcc ataccatgca acgcatgtgg tggtttattg gcgggtaaag aataacattg
    13741 cccaccagca ttcagcgtcg cattccccag tcgccctgcc cacggcacca ttgggaaaca
    13801 gccatactga aaagctctgc gctgcggctg ccattggcgt aatacttcag aaccaaatgc
    13861 ctttaacgag gttatcctgg caccatcatg cggataaatc tgcaggtgaa tattgccttt
    13921 ttccagcgtc actaactctc ctacaggccc aggccatggc gcgtcaaacg cctgttgtag
    13981 cgccaggtaa tgctcgtttt ttcgttcagt tttcatgaga ttgttccctg atgtagcggt
    14041 aagaagcggt ttcttgttca tcggttggct caatcagcca gacgcaaaca aaggaaagaa
    14101 ctgagacagc aaataaaaac agagcgacat accacggttt tcctccccct agcgccagca
    14161 aacttgaggc gataaggggg cttagtccgc caagaatcgc ggaaaattga taagcaaaag
    14221 aaaggccggt atatcgtact ttggtaccga acatgcgggt gaaaagtgtt ggctgcacgg
    14281 caaacatcat cgtcggacct aagttgtagc caaggatcat actgcaccag atgatgagcg
    14341 tacttttgct atccagtaat aagaagaaag gaaacgcgaa caggacacaa aatcctgctc
    14401 ccgacaaata cagacttttt tgacctatac gatctgataa ccatccaacc aatggacaac
    14461 tgaaaatacc gatcgccgag gcaatcaaca tacccgtcag gggaatatct cgcgacaatg
    14521 ctaattggct ggaaatatag acaataccaa aggcgttaat aatattagag gagacacttt
    14581 ccgccagcct cgcacccagt gccaataaaa tatttcgtgg atgttttttg aataattcaa
    14641 tcaacggagg aacggacttt tctttattat tcgtcgtttt ttgcttttga aaatccagcg
    14701 tttcttcggt atgcaggcgg ataaacagcc caacgatcaa catcaccgca gaaagccaaa
    14761 acggaatgcg ccatccccag gagagaaact gctccgaagt aagaaaatgg ttacagagag
    14821 caaaaatacc ggttgccagc ataataccca cagacgcagc cgtttgcggg agtgaaccta
    14881 aaaatccgcg tcgggattcg ggcgctgatt ctatggtcat caatgccgct ccgccgtact
    14941 cgccaccaag accaaagccc tgaatcaaac gcagcaccat taatacaagc ggtgcccaaa
    15001 tgccaatttc ctgatacgtc ggaataaaac caatcaaaaa tgtggagcaa ccgacaatag
    15061 ccagcgtcca gataagtgtt atttttcgcc cgattttatc gccaaaatga ccaaagacga
    15121 tgccacctaa aggacgagtc agaaaaccaa caccaaaggt cgcaaaggaa gctataagac
    15181 ctataaaagg atcgctgtct ggataaaaca gcggtgcaaa taccagtcct gctgccgtgc
    15241 caaaaataaa gaagtcatac cattccatta acgcgcctgc gaaactgccc ataacgacac
    15301 gtctcatggc tttaccggaa agcccttcac gcggtgcggg taacttttca gacataattt
    15361 tcctctcctg gtaggcgaga aatctgggat acgaaccact aaacgtgggc agtttgaatt
    15421 aatgcgcatc ggcttaaacc gatgcgcatt aaattatttc ttcaattcag ccagaacctc
    15481 tgcgtcctca gcatcaacat ttgcacccag tggttcccaa ttaatcaata cacaggtcag
    15541 gaatcccagg aatggcacga ttgccaacca gtaaaacgct tcagttttca acgcacccca
    15601 cagcatcggc cagaaaatga gtcctgcaat tgcccctgtg cgcataatag cgcgggcaaa
    15661 tccgacccca gtagggcgaa tagcgggagg ataagagagt gtggcaatgg tcatgcccag
    15721 cccaccagga cccgctgaat ggaagaaaat aattgatccc aataacccta aagacagcca
    15781 gggattagtc gttgccagtg cgcctaacga cagcaatgct aaggtcacaa cagcaaatcc
    15841 atacatcgac tgccggcgag tacccagtcg ttggagaata aacgagccga tcatcccgcc
    15901 tgtcacccca caaagggcat tcacgacggc agaaccggtt aacgcgccag taagcccgcc
    15961 acttattatt cccgccaacg taagaggaag ataaacacct acggcgttat attgccacgc
    16021 ctgcatggtt gccacgacac agccaagaat ggtgcgtttg cggtaacgat cattaaataa
    16081 gatgccatat ccaccactgt atttattctc tgctttttcc gagaatttcg cttctgttgt
    16141 acccgatgcc gcaacgcgag cctgaacacc ataacgtttt cccagaatgt cgcacgcttc
    16201 ctggtaacgc ccgacgcgtg cggcccacat tgcggattca ccaataaaga agtaacgcaa
    16261 aatcatgaag atgactgcaa aaatagctcc gatgaatatt ccgtaacgcc acaattgttc
    16321 ttcagcgata cccgacagaa gcagcggcaa aagcaccaaa taaacaacgg ttgtcgaaac
    16381 ataccaggcc atttgccaga ggttgaccga cgtgccggtt ttcttcgacg ttgaaccacg
    16441 aagctctgcc acggcattgg ttgccaaagg gaagtcgatg cctaaaccaa agcccatgat
    16501 gacgcggcac accagcaccc accagatact gggagcaata gctaccgctg cagcaccgat
    16561 ggtgcacagc cccatgccaa taataaatgc ccgcttttgc ccaaagcggt ttattactcg
    16621 gttcgctaat aaaccgccaa ttaacgcggc gattaatact gatgcattga cggttgcagc
    16681 aagacccggc gaaatgccga attgagcggc aatgtattta ttaccaaacc ccaccatcgc
    16741 cgcctggtag gcatcaatca atatgccgcc caaagcaata aaaataacgc cgatactggt
    16801 gttgattgca gagttcttat taacaatatc aattacgtcc tgtggccgac taattgaata
    16861 agttgcagga ctggtgattt gactcatcgc agtgtccttt ttaattgttg tagggtggcg
    16921 ctgacgtgac gcagcgccag acagagattt agaaaatcat tccggcgcaa attgcagcat
    16981 gacctttatg gagtcatttt gctgcgcgcg ttcgaatgcc gccgctgcat cctcaatggc
    17041 aaataaatcg gtaaccagtt cactggtatc aaaccgccca tccgccagcc attcgataac
    17101 agcatcgaaa tcttcaggga tgtacatgcc agagttgagc aagtcacgct cgaaacgttg
    17161 catcatcggt aatggtattt cacgcggccc acttggtacg cccatgcaca cgacggtgcc
    17221 tccggcatat acacgggtac atgcatcgtt aagcgtggcc tgcgctgcaa cgacatcaat
    17281 cgcccccgta aatcgcacat ctgcggccag ctcacctggt gcccaaactt ctgctgcgcc
    17341 cagttttaac gccagcgcgc gtttggcagc gtcaggttca atcacggtaa tcttacctgc
    17401 ccccataatg cgcagtgcct gcacgatgga taagcctatc gttccggcac caatcaccag
    17461 tacgctttcc agagaagctt tcggcattcg gttaacgcag tgacgagcac aagccgccgg
    17521 ttcggcaaag gccaacactt ttagcggtaa agaggctggt gcgacatggc aattacgcgc
    17581 aggaacaatg tgttgtgagc gagcaaagcc cggtgcgcgg aaaccagcaa cctgcggtgg
    17641 ttcacaaaga ttaaaacgtc ctgctttgca ggctcggcat tccatgcaag ccatgatggg
    17701 atcgaccaca acatgatcgc ccggctgtac atttttaacg tcgctgccca cttccgtaac
    17761 gcgcgctgca atttcatgac cggggacgac aggcggtttg gcaaacggat gaccgccttt
    17821 cagaacatgc agatccgaac cgcatatccc gtagaacacg ggcgcaatcc gtacttcgtg
    17881 cggtttaagt tgctggtgtg gtacttcaac atattgagtg gtgactttcc caatatctga
    17941 aaataaaacc tgggtaattt tatccattgt tattctccag aatcgatatc cataggggat
    18001 tagcgttgcg cgataccttc atcgacaatt tcagccagcc gttgcaagcg tggaacggag
    18061 acatcgcgca gcatggtttg tggatcttgt gcaccaacaa ccgacgccca aaccgctcgt
    18121 ccagccagga agcccgacgc accacctttc atggcaatac ttactgcacg gccaaaaata
    18181 tcggcatcaa cgccagaaga aagaattacc cacggcatct tcatctgatc gttaagttgc
    18241 tgacaggcag caagtaaggt tttttcatcg cctttgcccc ctaatggcat ctcggctttg
    18301 taaagatcag cctctgttcc accaagctct gcggcggcgg caacaatggc gctttcacga
    18361 tcaaagtccc aaccgcgacg aggtggacgc accacaggtt caataatgct aaccagcccg
    18421 gcactacggc agcgacgaac aaatttatca accattgcca gacgttcctc tgcgggttca
    18481 tcttcacgcc agagtacaag taatttcatt gccttagccc ccatttcacg ggctttatgc
    18541 gggtcgacgg acatatctat ttcaacgcta tcaaccggga tgccattacc tgggataaaa
    18601 agatcggcag cgacaatcag gccgcaagaa ttagccacag cgccctgctc gactatttgt
    18661 tcaagacaaa attgtttatc cgccagaacg gcagaagcat aaggagaaag gattcgggtc
    18721 gcggcaactt taaaatcggt gagtacactg tcggcgatgg gttttggctg ccctgctgcc
    18781 gcaaacatca ggcgcatggc ctcacgctga tcgacagcta acatggcgaa tccaccacct
    18841 gggcgggaga tatctttcag cgtaaatggg gtgaagtggg atgacataga aactcctgtt
    18901 aatagataaa attaaagacc agcctgctgg cgaacctgat ttagcagggc gctccagtcc
    18961 tgccgccctt tgccgtcttg acttgctgcg gcgtaataac tttctgctgc aatccccata
    19021 ttgagcggcg cccccgtttc ccgagcgacg tcaactgcaa tacgcagatc tttcaatgcc
    19081 aggtccacca tgaaggcggg agaaagatcg cctttcagca ctttgccagg ccaggtggtc
    19141 gtaaaatgtc ctttccctgc tgccgtacca ctcatcacct taattgcgac atcaagattt
    19201 aatcccagtg actcacataa cacagcggct tcggaggaca gcgcattaag cgcaatgctc
    19261 atatagttgt taattagctt cacgcggatc cccattcctg ggccaccagc ctcgaccagc
    19321 tcattaccca tgcacatgag aatcgggcgt gcacgcgcaa tctgctcatc agtgccgccc
    19381 gctaaaatca gcaatgtgcc atcaatcgca ttcactgacg ttctgcccac gggggcgtcc
    19441 atcatattga tgcctttttc ttgtaattcc cgtattaatg catcggtctg taagggatga
    19501 atagtcgaca tatcaatcac taacgcgtca gatgaaacag tttcacagac gcctttttcc
    19561 cccaacagca cctgtcgaac aatgtcccca ttgggaagca tagtgatgat aaattcagcg
    19621 cttgctgccg cttccgctgg ggtttgtgcc gcagtggccc cctgcgtaac tagcgcatcg
    19681 acggcctgtg tattgacatc aaatacctgt aatgaatgcc ctttttgcaa tagattgtta
    19741 gccattgggc tacccatctg ccccagcccg agaaatgcaa tggttgccat gaatactctc
    19801 ctgtctgaca ttgacaaaat aatgacaatt gaaaattcgt tttcgtctgt ttttgagcat
    19861 atttgtaatt tatgttcaaa aacacgacac gcatcactaa tttgatcatt tgtgtcattt
    19921 aaattgacca aagacgacaa aattaaacag gacaagacaa atatgaccag aattgcatgc
    19981 gtgggtatca cagttcagga caggatttat tctttgccca ctctgccgga gggcggcggg
    20041 aaatatcagg caaatcatta tcttgagatt ggtggcggcc cggcagcaac agccgctgtt
    20101 gcaatcgcaa agcttggcgt ggaggttgat ttcatcggtc gcgtcggcga tgacagttgt
    20161 ggaaatacct tacttgcaga acttgagggt tggggcgtca ataccgcatt ttgccgccga
    20221 tatcctaacg cccgttcgtc gcaatcagcg atcctcgttg atcagcatgg cgaacggatt
    20281 atcgttaatt atccaagtcc tgatctcggt actgatgccg aatggttaga agcgatcgat
    20341 ttctctcgtt atgatctgat tctggccgat gttcgctggc atagcggtac tgaaaaagca
    20401 ttctcgctag cgcgccttgc gggtgtgaca acgcttttag acgccgatat gacaccgcag
    20461 gatatttctc cacttgtcgc gcttgccgat cacgccgtgt tctcaacacc tgggctgaaa
    20521 cgcatgactg gcctgcagtc acctgaagaa ggtttgttcc aggcaacgac acaaacagca
    20581 ggtaaagtgt atgttacgct gggttctgaa ggatcgttgt ggattgaaga tggtcatttg
    20641 tgtcaacagg aggcattttc tgtcaatgtg gttgatacca ctggagcagg cgatgttttt
    20701 cacggcgcac tcgcggtagc gttggcagaa aaaatgccaa caaaagaggc tattcgtttt
    20761 gccagtgcgg ttgccgcgat gaaatgtacc cagcctggcg gtcgggccgg cattcctaat
    20821 cgtgagcaaa ccgaatcatt tttgtcactt tatgcgtaaa atggtaaaca tctaccgagg
    20881 aggggcaagg gagtattcat gagtatcatc gaagtgacag gtaatccacg ccatgaccaa
    20941 ctcgttcatc taatcgcaga acgcggttat atgaatattg aagaactggc acaactgctg
    21001 gatgtttcaa cgcaaaccgt gcgccgggat atccgaaaac tcagtgagca gggcttgatc
    21061 acccgtcatc atggaggggc cgggcgcgtc tccagcgtca tgaataccgc ttttgagcaa
    21121 cgggaacttt cgctcaccgc cgagaaacgg gcgatcgctg aggcagtcgc cgattacctt
    21181 cccgaacgct gtaccgtctt tatcaccatc ggtacaaccg tagaagccgt tgccagggca
    21241 ttactcaacc ggcgtgattt acgcattatc actaatagcc tgcgtgtggc acagattctt
    21301 tataagaatc aggatattga agtgatggtg ccgggaggca ctttacgcgc tcataacggt
    21361 gggattatcg gcccaggagc cgtggatttt attgaaggct tccgcgcaga ttatttaatc
    21421 accagtatag gtgccattga acatgacggc accctactgg aatttgatgt caatgaagcg
    21481 ttagtcgcga gaacgatgat taaacatgcg cggaatacat tgttagtggc cgatcatacg
    21541 aagtttaccg cgtctgctgc cgtttcaatt ggcaatgcac ggaatgtcag ggcttttttt
    21601 actgatgccc cgcctcccaa ttctttctgc cagttgttaa gtgaagagaa tgttgaactg
    21661 gtggttgccg agcaagaagt atcctgaaat tgaaacttcg gttgatacgg ttttcgttgg
    21721 ccggattaag cacatccggc cactaattaa gcttcagccg ccagcatctc tttcttacgt
    21781 tggctaatac gcttaaccag cattacggaa accaccgcac cacagaatgc gaagcaggcc
    21841 ataatgccaa acacgatttt gtagccaata atccccggat ttaaatccag aatgtagcca
    21901 tacagactga agcagaacat cgccggggcg taaccaataa agctacccaa cgccatcgcc
    21961 gcgcctgttt tattttcagc aatttttgct tcgccgatag gtgcaaaaaa tacggctcgc
    22021 tgtgtgaaga ctatagcgcc aaagcccagc gtacatgcca tccctaaata gaccggcata
    22081 ctttcgtgcg gcagcataat caacagtacg agcgcagcgg tacttatgat aaaggtgtag
    22141 catagatatt tactcggcga tttcaggatc ttatctgaaa tcatgccacc aatcggtcct
    22201 ccaatcattt tcagacagta ttggttaatg atgccgtaag cccccaccag agcaacgggc
    22261 aatagataga tgtttttcag gaatggaatg aagaatgtta ggccacagta aaccgcgtaa
    22321 acgaagaaga cgttgaaagc gataagccag atggttttat ctctcagcac cgaggtcata
    22381 ctggtgtttt gcgatgctcc gtcttctttt ttcacctcaa cggacggtgc ctcttctttg
    22441 tcattcagga caaagaaaat gataatgcct acggcaatca caataaggga atagaaccag
    22501 atgcctgctt taaaacccaa taagccactg ccaaaccagg taaatactgc caacgcagaa
    22561 aatgccacca cggtatcgac aatgccacgc cctgtttcga agaagccaaa caaccgacct
    22621 tgttgttcgc tattgcccaa tcgacttacc gacttgagca agaccggcca gttcatcatg
    22681 tcgcaagtaa cgccaaatag cgcccagaca aagaggatgc cccaataccc cggcattgtc
    22741 gtcaaataaa cacccagtaa tcctgtcgca atgagtgaga atgacatggt gtatctgcgc
    22801 ggtagtttat cggcaaaata aatagacaga aaaaagccca ctgtggtgac aaatgagttt
    22861 accgacatag cattaccaat ttgaccattg gtcaaatgga aatattcctg catcgggata
    22921 taaaacgcat ctttcagaga cggtaattta taaattgtac cgccacacaa tgttaataga
    22981 ctaaataacg cccatttctt tttcgtgagc ataacatccc ctaagcgatt tttaaaatca
    23041 ctgttttaaa ggcaataaga taccgaccat tcatatggaa tggttttatt aacagcgtat
    23101 gagttaaaac ttattagttg ttcagaagat ggtttgcctc ttgtttgatt tgttgtagta
    23161 cattacgcac atgatttatc tgattaattg catcttcttt ttctttacgt aaccgcgcat
    23221 ccagtaaatg attttctggc aatggtgttt cactcatctg gcgataaggg atccacggat
    23281 tatcatcttc gtcttcaaag cggaacgccg gcgcatagta atcaacctct tcttccaggc
    23341 catatgccgt cacctgcggc tcatcatcac ccaggcagat aaggtgcgtt aataacgctt
    23401 tgaacggcat atcaccctga cctgaaatac aggctttatg acccaggcca ccctcttctt
    23461 taacgatcaa ggcatctttg atatggacct gggtaatatg cggtgccatc gtttttaaag
    23521 cgtcgatggg atgctcattt gcattgatca tgttcgcaaa atcaaacagt aaggaaagag
    23581 attccatctc actttctttg accagcgaca ccagctcatg gctttttaaa tcttcatgct
    23641 gttcgatcgt aaaagtcagg ccgctgtcct gatacgtttc ccgtacatag gcaatgtcgt
    23701 tagcgatgat cgataatacg tcgcgcaaat taccttcata acgtggataa aaacgtacgg
    23761 acgatgcccc agttttcaac gcaatggcga cggcttcgtc aatagatgcc ttatctgaag
    23821 cgctggtttc aatatgaata tcaaggttca gacggcgggc tttatcccca aaggcagaga
    23881 gttctttatc gtccatatta ccaagagaaa aacgctcgcc atccagaaca tggattttga
    23941 cgccacgtag attattttca cttgcgatat caagtaaatc agcaggcaac actctttcca
    24001 gccgcatatt taaatgaaag gcataagcat gtaaataaag aggaagagtg tcgacacgtt
    24061 gtagaatctt tcttgcgtta tttattgtca ccataaaaca cctacagatt aatgatgagg
    24121 tctacggcct caaatcgcca atattcaata tctatctgga caatattgcc ttgctggtcg
    24181 gcgcgatgct tttcaattaa gatcgctggc atacccgcag aaccgccaag atacttgctg
    24241 atatcgcccg gcattctgac gggtttgaaa atcaattttt ttactaccgt ctctttttgg
    24301 tagcactttt cccagactgc agaaaatgag tggttttcaa gttgttcaat aaaacccggg
    24361 gcaacttcag gattaatata tgtttcgtga tagaaaactt tatgtccttc cagcgctccc
    24421 cagccggtga tgcgatagag ttcagttgat ggtgtcactg taatcagtgg cacgagcgtc
    24481 tcgggaatat ctctggtacg gcttttctcg gtaaaccccc aggaaggctc ccgcccttgc
    24541 tcaattgcag cccgctgaaa gctcgccgac agctctggac tgtaattaaa tcgcggctgg
    24601 gtcacgaacc accctttacg atccttacgg aagattttgg attctgcctc gaggttcaac
    24661 aacgcctggc gcagcgtcat acgtttaatg cccagcagtt ctcccagctc tctttctgaa
    24721 gggagttttc cacccggcgt ggtgatgcca tctttcaacc accgatccag tttctctttt
    24781 gcattttcta cggtggattg attctccgcc attgtttaat ccttttggtt taaaccagat
    24841 gaggcatctt acgcttacgc caaaaaagaa agcaattaac ggcgtatgac tttgcagata
    24901 attgtgacaa agctctctat tgacgtaatc acagacttat aagggagata ctcgcattgg
    24961 tgtgcgctca cgacaggtaa aaaaaaacct gccagcgatg gcaggttttt tatgactgaa
    25021 aagcgaaatt aatcgtcttt cggtgagcgg ttggcgcgac gacgatcgtt ttccgtcagg
    25081 tgacgtttac gaatacggat agaggtcgga gtcacttcta ccagttcgtc atcatcgatg
    25141 aactccagag cttgttccag agtcatgcgg ataggcggaa ccagaacaac ggcttcgtca
    25201 gtaccggaag cacgcatgtt ggtcagtttc ttaccggtca ggcagtttac agtcaggtcg
    25261 ttagagcggc tatgaatacc gataatctga ccttcgtaaa cttctgcacc gtgaccgagg
    25321 aacagcttac cgcgatcctg cagaccgaac agcgcgaacg cgaccgcttt accctgaccg
    25381 ttagagatca gtacaccgtt ctgacgctga cccacttcgc ccggacgtac gtcgtcgtag
    25441 tggctgaagg tggagtacag cagaccagta ccggaagtca tggtcatgaa ctcagaacgg
    25501 aagccaatca gaccacggct tgggatcacg tagtcgagac gtacgcggcc tttaccgtct
    25561 ggattcatgt ttttcaggtc gcctttacgt tcgcccagcg cctgcatcac agaaccctga
    25621 tgctgttctt caacgtccag cgtcacgttt tcatacggct cttgtttacg accgtcgatt
    25681 tcacggaaga ttactttcgg acgggatacc gccagttcga aaccttcacg acgcatgttt
    25741 tcgataagaa cagacaggtg cagttcgcca cgaccagaaa cgcggaacgc atcggcgtct
    25801 tcggtttctt ctacgcgcag cgcaacgttg tgtaccagtt ctttgttcag acgatccagg
    25861 atctgacgag acgttacgaa cttaccttct ttaccgcaga acggcgaggt gttaacgcag
    25921 aagaacatag aaacggtcgg ctcatcaacg gagagtgccg gcagcgcttc aacgttttgc
    25981 gtgtcgcaaa cggtgtcaga aatgttcagt tcgcccaggc ccgtgatcgc cacgatatcg
    26041 ccagcttccg ccagatcggt ttcgatacgt tccagaccga ggtggcccag cactttaccg
    26101 actttcgcgt tgcgggtttt gccttcgcta tcgatgatag taacctgctg gttcggcttc
    26161 actttaccgc gcttgatgcg gccaatgccg ataacgccaa catagctgtt gtaatcgagc
    26221 tgagaaatct gcatctggaa cggaccgtca aggtcaacgt ccggcgcagg aacgtggtca
    26281 acaatcgcct gatacagcgg ggtcatgtct tccgccatat cttcgtggtc cagacccgcg
    26341 ataccgttca gcgcagaagc gtaaacgatc gggaagtcca gctgctcgtc ggtcgcgtcg
    26401 aggttaacga acagatcgaa tacctgatcc acaacccaat caggacgcgc gccagggcgg
    26461 tcaactttgt tgataacaac aatcggcttc aggccgtaag caaacgcttt tttggttacg
    26521 aagcgcgttt gcggcatcgg gccgtcaaat gcgtcaacca ccagcagcac tgagtctacc
    26581 atggacatta cacgttcaac ttcaccaccg aagtcggcgt gccccggggt atcaacgatg
    26641 ttgatacggt aatcattcca tttgatagcg gtgtttttcg cgaggatggt aatcccacgc
    26701 tctttctcca aatcgttgga gtccatcacg cgctcttggg tttcggcacg agagtcgaac
    26761 gtaccggatt gttggagcag cttgtctacc agggtggttt taccatggtc tacgtgcgcg
    26821 atgatggcga tattacgcaa tttttcgatc acaactttgc ctcaggcatt agaaatagcg
    26881 cgttattgta cacggattaa tcgcactaca aaacaggatc acaaacatcc tccgcaaaca
    26941 agtattgcag agtccctttg tgatcgcttt cacggagcat aaaaagggtt atccaaaggt
    27001 cattgcacca atatggtgct taatgtttcc attgaagcac tatattggtg caacattcac
    27061 atcatggtgc agcccttttg cacgatgtta agcatgataa cgccttttag gggcaattta
    27121 aaagttggca cagttttcgc tttatctttt tacggcgaca cggccaaaat aattgcagat
    27181 ttcgttacca cgacgaccat gaccaatcca ggagagttaa agtatgtccg ctgaacacgt
    27241 actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca ccgataccaa
    27301 aggtaaagaa cagcacgtca ctatccctgc tcatcaggtg aatgctgaat tcttcgaaga
    27361 aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg aatccgacat
    27421 ggtgctgatg ccagacgcat ccaccgcagt gattgacccg ttcttcgccg attctaccct
    27481 gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc gtgacccgcg
    27541 ctctattgcg aagcgcgccg aagattacct gcgttccact ggcattgccg acaccgtact
    27601 gttcgggcca gaacctgaat tcttcctgtt cgatgacatc cgtttcggat catctatctc
    27661 cggttctcac gttgctatcg acgatatcga aggcgcatgg aactcctcca cccaatacga
    27721 aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg ttccgccggt
    27781 agactctgct caggatattc gttctgaaat gtgtctggtg atggaacaga tgggtctggt
    27841 ggttgaagcc catcaccacg aagtagcgac tgctggtcag aacgaagtgg ctacccgctt
    27901 caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgttg tgcacaacgt
    27961 agcgcaccgc ttcggtaaaa ccgcgacctt tatgccaaaa ccgatgttcg gtgataacgg
    28021 ctccggtatg cactgccata tgtcgctggc taaaaatggc gttaacctgt tcgcgggcga
    28081 caaatatgca ggtctgtctg aacaggcgct gtactacatt ggcggcgtca tcaaacacgc
    28141 gaaagcgatt aacgccctgg caaacccgac caccaactct tacaagcgtc tggtcccggg
    28201 ctacgaagca ccggtaatgc tggcttactc tgcgcgtaac cgttctgcgt ctatccgtat
    28261 tccagtggtt tcttctccga aagcacgtcg tatcgaagta cgtttcccgg acccggcggc
    28321 taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgacggta tcaagaacaa
    28381 gatccatccg ggcgaagcga tggacaaaaa cctgtatgac ctgccgccgg aagaagcgaa
    28441 agagatccca caggttgctg gttctctgga agaagcactg aacgaactgg atctggaccg
    28501 tgagttcctg aaagccggtg gcgtgttcac tgatgaagcg atcgatgcgt acatcgctct
    28561 gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcac ccggtagagt ttgagctgta
    28621 ctacagcgtc taatagttga agttgtacta cccggcgcaa caacgccggg atttagttgc
    28681 cgtggaaact ttcagcccat ctctgcatgg gcttttttct ccgtcaattc tctgatgctt
    28741 cgcgcttttt atccgtaaaa agctataatg cactaaaatg gtgcaacctg ttcaggagac
    28801 tgctttatgg caacaggcac gcagcccgat gctgggcaga tcctcaactc gctgattaac
    28861 agtattttgt taatcgatga caacctggcg atccattacg ccaaccccgc cgcgcaacaa
    28921 ctgctcgccc aaagctcccg caaattgttt ggtacaccgt taccggaact gttgagctac
    28981 ttctcattaa atatcgagct gatgcaagaa agtctggagg cggggcaagg ttttactgat
    29041 aacgaagtga cgctggtcat cgacgggcgc tcgcatatcc tttctgtgac ggcccagcgt
    29101 atgccggacg gcatgatcct gctggagatg gcaccgatgg ataaccagcg ccgcttaagt
    29161 caggaacagc tacagcacgc ccagcaggtt gctgcccgtg atttagtgcg cggcctggca
    29221 catgagatta aaaatccgct tggcggttta cgtggcgcgg cgcagctgct cagcaaagca
    29281 ttacctgacc cgtcactact cgaatatacc aaagtgatta tcgaacaggc ggaccggctg
    29341 cgaaatctgg tcgaccgtct gttgggaccg caactgcccg gtacgcgcgt taccgaaagt
    29401 attcataaag tagctgaacg cgtggtgacg ctggtgtcga tggaactgcc ggacaacgtg
    29461 cggttgattc gggattacga ccccagcctg ccggaactgg cgcacgaccc ggatcaaatt
    29521 gaacaggtct tgctgaatat tgtgcgcaat gcgctacagg cgctggggcc ggagggtggt
    29581 gaaatcattc tgcgtacccg cacagcgttt caactgacct tacacggcga gcgctatcgg
    29641 ctggcggcgc gaattgatgt ggaagataac gggccaggta ttccgcctca tttgcaggat
    29701 acgctgtttt acccgatggt cagcggccgc gaaggtggca ccgggcttgg cttatccatc
    29761 gcccgtaatt tgattgatca gcattcaggc aaaattgaat ttaccagttg gccaggtcat
    29821 accgagttct cggtttacct gcctatcagg aaataaaggt gacgtttatg caacgaggga
    29881 tagtctgggt agtcgatgac gatagttcca tccgttgggt gcttgaacgt gcgctcgctg
    29941 gagcgggttt aacctgtaca acatttgaga acggcgcgga ggtactggag gcgctggcga
    30001 gcaaaacgcc ggatgtgctg ctttcagata tccgtatgcc gggaatggac gggctggcgc
    30061 tgctcaagca gattaaacag cgccatccga tgcttccggt catcattatg accgcacatt
    30121 ccgatctgga tgctgccgtc agcgcctatc aacaaggggc atttgattat ctgcccaaac
    30181 cgtttgatat cgacgaagcc gtggcgctgg ttgagcgcgc catcagtcat taccaggaac
    30241 agcagcagcc gcgtaatgtt cagcttaacg gcccaacgac cgatatcatc ggtgaagcgc
    30301 cagccatgca ggacgtgttc cggattatcg gtcggctttc gcgttcttct attagcgtgc
    30361 tgattaacgg cgagtccggc accggtaaag aactggtcgc tcatgccctg catcgccaca
    30421 gtccgcgagc caaagcgccg tttatcgcgc tcaatatggc ggctatcccg aaggatttga
    30481 tcgaatccga actgtttggt cacgagaaag gcgcatttac cggcgcgaat accattcgtc
    30541 aggggcgttt tgaacaggct gatggtggca cattattcct cgatgaaatt ggcgatatgc
    30601 cgctggatgt gcagacgcgt ttgctgcgcg tactggcaga tggtcagttt tatcgcgttg
    30661 gcggctatgc gccggtgaaa gtagatgtgc ggattatcgc tgccactcac cagaatcttg
    30721 aacagcgggt gcaggaaggt aagttccgtg aggatctgtt ccaccgcctg aacgtcatcc
    30781 gcgttcatct gccgccactg cgtgagcgtc gggaagatat tccccgtctg gcacgccatt
    30841 ttttacaggt tgccgcgcgc gaactgggcg tagaagcgaa gttgctgcat ccggaaaccg
    30901 aagccgcgct gacgcgcctg gcgtggccgg ggaacgtgcg ccagctggaa aacacctgtc
    30961 gctggctaac ggtgatggcc gccgggcagg aagtgttgat tcaggatttg cccggtgaac
    31021 tgtttgaatc aacggttgcg gagagtactt ctcaaatgca accggacagt tgggcgacac
    31081 ttttagcgca gtgggcagac agagcgctgc gttccggtca tcaaaatctg ctttccgaag
    31141 cgcagcctga gctggagcgg acgttactga cgaccgcgtt gcgacatacg caagggcata
    31201 aacaggaagc ggcgcggcta ctcggctggg ggcgtaacac tctgacgcgg aagttaaaag
    31261 agctggggat ggagtgattc acggcttgtg tgtaaagatt gattattgag cgcaaattgc
    31321 tggtgttttg cgctttactg ttccgataag ttcagtatga tcttgcccgg aaaacgggga
    31381 gagtcattat gctggaatca ataattaatc tggtatcgag tggcgcagtt gacagccaca
    31441 caccgcaaac tgctgttgct gccgtgctgt gtgccgcgat gattgggctg tttagttgag
    31501 tttcagattg ctgatgaagt gctctttgtt catgccggat gcggcgtgaa cgccttatcc
    31561 ggcctacaaa agcaatattt tcaacagatt ttgcaggcct gataagcgta gcgcatcagg
    31621 caatcttgtt ctcagcacat tgatggcgct ttcgttttct actttgtaaa cgaagcgcca
    31681 ttcacaattt aaatcacccg agagaactgc tgcatccgcg ctttctggcg cagataggta
    31741 tcaaagcaca tgcaaatgtt gcggatcagc aagcgacctt tcgccgtcac ctgtattccc
    31801 ttctcatcca catccaccag cccatctttt gctaacgggg cgagcagctt gagatcttcg
    31861 gcaaagtaat cagcgaagag caaatcccac tgttgctcaa tgggagcgta atccagacgg
    31921 aagttgcaga tgagcgactt aatcacatcg cggcgaatac agtcatcacg cgttagcgca
    31981 ataccacgcc acagcgcatt gccttgttca tccacttgct gatagtactg cttcaactct
    32041 ttctggttct gcgcgtagca gtcgccaatc atgctgatgg cggaaacgcc catccccagc
    32101 agatcggtat cgccctgagt ggtgtagccc tggaagttac gatgcagcac gccttcacgc
    32161 tgggccaccg ccagctcgtc atccggacgg gcaaagtgat ccataccgat aaactgatag
    32221 cccgattgtg tcaggaaggc gatggtttcc tgcaggatat cgagtttttg ctgcggactc
    32281 ggcaggtcag catctttgat tttgcgctga gcagcaaaaa tggtcggcag atgcgcgtag
    32341 ttaaagacac tcagacgatc ggggttcagc tccgccacac gtttcagggt aaaggcgaaa
    32401 ctctccggcg tctgtttcgg caggccgtaa atcaggtcga tgttggtgga ggtaaagcca
    32461 atctcacgcg catggttaag cagtgcaaag atgaactctt catcctgctc gcggttaacc
    32521 agacgttgca cttctttgtt gaagtcctgc acgcccatgc tcaggcgatt aaagccttcg
    32581 gcgcgtaaat gatcgagtac atccagttcg atttcccgcg gatcgacttc gatcgagatc
    32641 tccgcatcgg cattgaactg gaagttttcg cgcagcagct tcatcagacg gctgatttgc
    32701 gctttattca gatacgtcgg cgttccgccg ccccagtgca attggctgac gtgacgcccg
    32761 gcgaacagcg gtgcacgatg gacgatttct tgctccagcg cgtccagata ctgatcggcc
    32821 ttgtgctgct ggcgagtaac tatcttattg caaccgcaga agtaacaaag cttatggcag
    32881 aacggaatat gtacgtagag agataatgga cgctcaggat agcgcgccac ggcttgtaaa
    32941 aacgcctgtt cgccgaagtc ttctgaaaac tccagcgcgg tcgggtacga ggtgtatcgt
    33001 ggcccggaat agttatattt ctggatcagg gccagatccc agtcgatttg ctgtacagac
    33061 atgctcactc cttccgatgg cgtctctggc ggctacggcg taccggcgga tgcccgtttc
    33121 gagtcatcag ccggttgcgc agccactttt gtcgccgcga caaccgctgt agtttaacga
    33181 ataaccacac cagataacat ataaccggaa gggttatcag cagaacggga aggcccacgg
    33241 cggcaatccg ttagttgccc cgcagcagac gcatcatgtc ttcttgcttc tcgtcttctt
    33301 cctcttcttc gtcatcgtca taagagaggc cgagtttctg catcaactca tcaatacgat
    33361 ccagtttggc atccacccag gattgctctt cggcgctcag ggtttcgcct gcttccagac
    33421 gttccagcag cgcatccaga cgctcatccg tttccagtaa ctccaactcc gcctgcggtg
    33481 aaagcatagg tttctcactc ttcggtttgt gctgtttggt gactttttca gtcacgccca
    33541 atggaatagg ggttttactg ccaatacgtg gatctttcgg tgcgttctgg cctttgctgc
    33601 ctgacgtggt gttaccgccc gctgcgcggc tgcccggcgc atgaccacga cgttttttct
    33661 gacgcttacg gtcacgagct tcctgatcca gctcctcgcg tgtttttcga cgcgcttttg
    33721 catgaccttt gctgcgtgag tttgaagatg atggtttcat aatggttcgt cttttagccg
    33781 ttttattcag tatagatttg cggcggaatc taacagaaag caagcaaaga aaaaaggcga
    33841 cagattactc tgtcgccttt tttcctgact cataaccctt aacgggtctt acaatccttg
    33901 caggccgcca acacaccctg tttttccatt agccggaaca atccgtgttg attccatttc
    33961 cgtttaatta cgtctccgga cgtttatctt cctgacaatc ctctgtcttc gcctcctggc
    34021 gctcctgacc ctcatcctga gtcattgttc ctgttagcgt cctcgctcta tgggtgctac
    34081 tttacgcctt tgctttaaat aaacaatcaa ccgccatcag tatttgcaca ttttcagagt
    34141 tttatataaa agcaattcac tgatttatat agcttatttg acatacgtcg cagaattttc
    34201 tctgctattt cgcataaaga ctatcggcaa tgtctcacaa agcatatggc ttttacttac
    34261 aacgctcacg ccgaaaaatc cgccttttat tatggttcag gtataatcgc cgcaaagctt
    34321 tgactgatgg agacgaccgc tttgactaat ttgaattatc aacagacgca ttttgtgatg
    34381 agtgcgcctg atattcgcca cctaccttcc gataccggaa ttgaagtggc ttttgcaggc
    34441 cgttccaacg caggtaaatc cagcgcgctg aacacgctga ctaaccagaa aagcctggct
    34501 cgtacctcaa aaaccccggg gcgcacccag cttatcaacc tgtttgaagt ggctgacggc
    34561 aagcgtctgg ttgacttgcc tgggtacggt tatgcggaag tcccggaaga gatgaagcgc
    34621 aaatggcagc gcgcgctcgg cgagtacctc gaaaaacgtc agagcctgca aggtctggtg
    34681 gtgctaatgg atattcgcca tccgctgaaa gatttggatc agcagatgat tgagtgggcg
    34741 gtagacagca atatcgccgt gctggtactg ctgaccaaag cggacaaact ggcaagcggc
    34801 gcacgtaaag cgcaattgaa tatggtgcgt gaagcggtgc tggcgtttaa cggtgatgtg
    34861 caggttgaaa cgttttcttc gctgaagaaa caaggcgtgg acaagctgcg gcagaaactg
    34921 gatacctggt ttagcgagat gcagcctgta gaagaaacgc aggacggcga ataattttct
    34981 tgccttaatg cttgtgccgg atgtggcgta tcaggcatta cggatctttt ctttcgccca
    35041 ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc aaatccgatt
    35101 acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac gcgactaact
    35161 ttactctttt ttgttcagtt cagaaagcat tttttgtaat tttttttcag ttgttgcata
    35221 ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta cgaaaaaagg
    35281 gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc cacttccacc
    35341 agcagcggca catccagacg ggtacagttt tccatcagtt gatgaatctg cttcgcgacg
    35401 gcatcgacat catctttatg aacttcaaat accagttcat cgtgtacctg catgatcata
    35461 cgcacacgcg gttgctcagc ctgtagccac gcatcaacgg caatcatcgc ccgtttgata
    35521 atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc cgctgcacga
    35581 cgagccccat tgctggattt gatatccggc agatacagac ggcgtccgtc cagcgtttca
    35641 acgtagccct gctctttcgc ctgagcacgg gtgcgttcca tatactccag cacgccaggg
    35701 tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat gttcaattgc
    35761 cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc tttcgcgcta
    35821 cggcgttgct cgctggttac ggtttccagt ggcaaaccaa acacttctgc cgccgtagcc
    35881 cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttgtcacg cgagagatgc
    35941 gccataatgc gcagttcaat ctgcgagtag tccgccgaga caatcacata atcctctggc
    36001 gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat gttttgcagg
    36061 ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata agaggtatgc
    36121 acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt cgatttcagc
    36181 ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag cgccagttct
    36241 tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag cggtttaatt
    36301 ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aggaaaggtt aaattcttca
    36361 cctgcaattt catgcgcttt cttttccagc tcagccagac gaagagtgag ctcttcagaa
    36421 tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg tgaaagcacc
    36481 ggcactagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg tttttgcaga
    36541 tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc ggcgtaacgt
    36601 ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt acccgcaatc
    36661 tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc catatcgtga
    36721 cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc aatcccacgc
    36781 agctcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg cccaaccttc
    36841 agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc gcgagagatt
    36901 tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac gcctggctcg
    36961 atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc ggtttcggta
    37021 tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag tgtttcttca
    37081 tcaaggatgg tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc tggcgcttcg
    37141 tctacaacac tggtttcctg cggcttcgcg gctggttttg cccctttggc ctgtaaccat
    37201 ttgcccgctt cgacatcagc agtccagcgt ttgaactcat actttttgaa cagccctaac
    37261 aactcttctg ccaccggtgg ctgcacttcc agttgttcac aggtcagctc cagttcaacg
    37321 tcggttttaa tcgtcgccag ctggtaagag agataagcca cttctttgtt ttgctcgagc
    37381 ttcgccgcca ttgttttcgc gccacggaag ctcaacccag caattttttc tggctcggca
    37441 tacagcgtat ccagtccgcc aagaccttgt agcaatgctt gcgcggtttt ttcaccgacg
    37501 cccggtacgc cagggatgtt atcagaggag tcacccatca gcgccaggaa gtcgatgatc
    37561 agttctggcg gcacgccgta cttattcacc acctcttccg ggccgaggat ggtattcgtc
    37621 atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt atcgcctgtg
    37681 cttatcagca ccggacgccc ggctttttcg gcttcccgcg ccagagtacc gataacgtcg
    37741 tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac catcgcgtgc
    37801 aagggttcga tttgtgcacg cagatcgtcc ggcattggcg ggcgatgtga tttgtaatgt
    37861 tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc tgcatgcgtc
    37921 ggtttatatt gcatgattag actgcgcagc atgttgagga caccatacat cgcaccggtc
    37981 ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata aagataagat
    38041 gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc cgtgcctgtt
    38101 tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt ttcaccgctt
    38161 ttcgtgaaaa tcttttgtag atcttctgga tcgctcgcaa atcgtcatgt ggataacttt
    38221 gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag catgatattt
    38281 gtctttgctt ttcatacagt taaatataaa gccatcccta ataattgtga taatgggatt
    38341 ttttctaatg tggatatata tgaattattt ttctccttaa ggatcatccg ttatttgggg
    38401 cgttttctgc taaaatcggc gccttgcgct tctttagcgc actgtctatg gctaaccttt
    38461 tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg agtatcgtat
    38521 tgaccatttt ggtcactatt ttttgctccg tcccgatcat cattgccggg attgtaaaac
    38581 tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat tttatgatgt
    38641 attgctggtg tgaaagtctg gcggtattac tgcatctgaa cccacacctg caatgggaag
    38701 ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt aaccaccgta
    38761 gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg atgaataaat
    38821 atttcctcaa gcagcagctt gcctgggtgc catttcttgg cctggcatgc tgggcgctgg
    38881 atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg gaacgacgtg
    38941 gtaaagatgt tgaaaccacc cggcgttctt gtgaaaagtt tcgcctgcac cccaccacca
    39001 ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa acgcactcca
    39061 cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta aatgtactgg
    39121 gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat aaccgtcagc
    39181 cgttcttcga tatgttaagc ggtaaactga cgcgaattgt cgtacatgtg gatttgcagc
    39241 ctatttccga tgagttacat ggcgactaca tcaatgacaa aagctttaag cgccattttc
    39301 agcaatggct gaactcactg tggcaggaaa aagaccggct tctgacgtcg ttgatgtcat
    39361 cacagcggca ggacaagtaa attttgctcg cctctgaatg agcagaggcg agtgagtatt
    39421 ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct cctgtccgtt
    39481 aaaaacaact ttacccggtg tataacgcag ttgcagcgag gcggtattgt tgtcaatcgt
    39541 gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat cagcccgctt
    39601 ttgcgctttt tcagcatcca ttccttcaga taaattaagc tgttttgcgg tttctgtgac
    39661 aacattaagc ggtaacttca catcaaaaat gagcgattta atatctttgt ttgtggatga
    39721 tgaggatttg gctgggtcgg caatactgat atccagattg gcatttagtt cgccgagtgc
    39781 attcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata acggtaaata
    39841 ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg ctaactcagg
    39901 gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgacaaatt ggcgcaccgc
    39961 tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt gctgattccc
    40021 ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt tgtctttaac
    40081 gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt ctccctgatt
    40141 gataatgtta atctgattga atttcgactc gctttcgcca accggaaatt tcttttcttc
    40201 taatcgcgtt agagaatcaa tggtaaaact tttcgccgtt actttattca atggcgataa
    40261 ttccagaaca agatcttcga ctttgccttc aatatccaga ttagacaatg aactaccatc
    40321 accattaaaa gtaaactggc cctcaccaaa agaaaacttt ccatattttg caggggcaac
    40381 atctaatgtc gtggagaatg agccaccaaa cgcgaatcgc aatgtcgctt ccataggaga
    40441 tttatttttt gcggcgataa atagcggttg tgtcagttca ttatccacta atttcacttt
    40501 tgccgctgcc agtgcgggga tgacattacc acgcattaac attgtgatgg gtaacggacc
    40561 gtgctcaaca tccacatcaa acgcaacttt ctggccaggt ttgatattga gatcaggtgc
    40621 accgttatca aatgtcattt gcatttgaaa acgagaatta aaaaagcctt tgtcaaaatt
    40681 tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttagcat cgttaaaatc
    40741 tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg tgccaccacc
    40801 ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca tataaatatt
    40861 ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt ccagcggcag
    40921 gacgcattat cagaatgtat ttatatttat taaata
//
LOCUS       DAZGHF010000026        40219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGHF010000026 DAZGHF010000000
VERSION     DAZGHF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.026"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1212)
                     /locus_tag="ACHK3A_001513"
     CDS             complement(<1..1212)
                     /locus_tag="ACHK3A_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8739617.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELKIIELQMKINNLI
                     VALSVAPEVTAI"
     gene            complement(1270..1419)
                     /gene="ylcJ"
                     /locus_tag="ACHK3A_001514"
     CDS             complement(1270..1419)
                     /gene="ylcJ"
                     /locus_tag="ACHK3A_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YlcJ"
                     /protein_id="HFO8739618.1"
                     /translation="MSLVLCFLLMSLFFMYSFVLSRLWRKKIAIRLLLYIQDNVTLIV
                     FLNKN"
     gene            complement(1467..1799)
                     /gene="emrE"
                     /locus_tag="ACHK3A_001515"
     CDS             complement(1467..1799)
                     /gene="emrE"
                     /locus_tag="ACHK3A_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO8739619.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(1867..2169)
                     /locus_tag="ACHK3A_001516"
     CDS             complement(1867..2169)
                     /locus_tag="ACHK3A_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO8739620.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(2166..2867)
                     /locus_tag="ACHK3A_001517"
     CDS             complement(2166..2867)
                     /locus_tag="ACHK3A_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO8739621.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2864..3883)
                     /locus_tag="ACHK3A_001518"
     CDS             complement(2864..3883)
                     /locus_tag="ACHK3A_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO8739622.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(3880..4419)
                     /locus_tag="ACHK3A_001519"
     CDS             complement(3880..4419)
                     /locus_tag="ACHK3A_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO8739623.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(4489..4719)
                     /locus_tag="ACHK3A_001520"
     CDS             complement(4489..4719)
                     /locus_tag="ACHK3A_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8739624.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            4824..5513
                     /locus_tag="ACHK3A_001521"
     CDS             4824..5513
                     /locus_tag="ACHK3A_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO8739625.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            6109..6315
                     /locus_tag="ACHK3A_001522"
     CDS             6109..6315
                     /locus_tag="ACHK3A_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO8739626.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            6391..6687
                     /locus_tag="ACHK3A_001523"
     CDS             6391..6687
                     /locus_tag="ACHK3A_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO8739627.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            6693..7478
                     /gene="bet"
                     /locus_tag="ACHK3A_001524"
     CDS             6693..7478
                     /gene="bet"
                     /locus_tag="ACHK3A_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO8739628.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIHASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            7475..8155
                     /locus_tag="ACHK3A_001525"
     CDS             7475..8155
                     /locus_tag="ACHK3A_001525"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO8739629.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            8152..8334
                     /locus_tag="ACHK3A_001526"
     CDS             8152..8334
                     /locus_tag="ACHK3A_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO8739630.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            8307..8498
                     /locus_tag="ACHK3A_001527"
     CDS             8307..8498
                     /locus_tag="ACHK3A_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO8739631.1"
                     /translation="MHKASPVELRTSIEVAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            8575..8790
                     /locus_tag="ACHK3A_001528"
     CDS             8575..8790
                     /locus_tag="ACHK3A_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO8739632.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            8889..9098
                     /locus_tag="ACHK3A_001529"
     CDS             8889..9098
                     /locus_tag="ACHK3A_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO8739633.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQ"
     gene            9155..9433
                     /locus_tag="ACHK3A_001530"
     CDS             9155..9433
                     /locus_tag="ACHK3A_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8739634.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            9405..9470
                     /locus_tag="ACHK3A_001531"
                     /pseudo
     CDS             9405..9470
                     /locus_tag="ACHK3A_001531"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            9632..10795
                     /gene="intD"
                     /locus_tag="ACHK3A_001532"
     CDS             9632..10795
                     /gene="intD"
                     /locus_tag="ACHK3A_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO8739635.1"
                     /translation="MSLFRRNEIWYASYSLPGEKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(10811..10887)
                     /locus_tag="ACHK3A_001533"
     tRNA            complement(10811..10887)
                     /locus_tag="ACHK3A_001533"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10851..10853),aa:Arg,seq:tct)
     gene            11158..12024
                     /gene="folD"
                     /locus_tag="ACHK3A_001534"
     CDS             11158..12024
                     /gene="folD"
                     /locus_tag="ACHK3A_001534"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO8739636.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            12026..12238
                     /gene="ybcJ"
                     /locus_tag="ACHK3A_001535"
     CDS             12026..12238
                     /gene="ybcJ"
                     /locus_tag="ACHK3A_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO8739637.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            12346..12867
                     /gene="ybcI"
                     /locus_tag="ACHK3A_001536"
     CDS             12346..12867
                     /gene="ybcI"
                     /locus_tag="ACHK3A_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8739638.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHKVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(12903..14288)
                     /gene="cysS"
                     /locus_tag="ACHK3A_001537"
     CDS             complement(12903..14288)
                     /gene="cysS"
                     /locus_tag="ACHK3A_001537"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO8739639.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            14462..14956
                     /gene="ppiB"
                     /locus_tag="ACHK3A_001538"
     CDS             14462..14956
                     /gene="ppiB"
                     /locus_tag="ACHK3A_001538"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO8739640.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            14959..15681
                     /gene="lpxH"
                     /locus_tag="ACHK3A_001539"
     CDS             14959..15681
                     /gene="lpxH"
                     /locus_tag="ACHK3A_001539"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO8739641.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            15799..16308
                     /gene="purE"
                     /locus_tag="ACHK3A_001540"
     CDS             15799..16308
                     /gene="purE"
                     /locus_tag="ACHK3A_001540"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO8739642.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            16305..17372
                     /gene="purK"
                     /locus_tag="ACHK3A_001541"
     CDS             16305..17372
                     /gene="purK"
                     /locus_tag="ACHK3A_001541"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO8739643.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPVQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(17705..18598)
                     /gene="ybcF"
                     /locus_tag="ACHK3A_001542"
     CDS             complement(17705..18598)
                     /gene="ybcF"
                     /locus_tag="ACHK3A_001542"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO8739644.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTKDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(18595..19410)
                     /gene="ylbF"
                     /locus_tag="ACHK3A_001543"
     CDS             complement(18595..19410)
                     /gene="ylbF"
                     /locus_tag="ACHK3A_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO8739645.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVMAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFIHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKS"
     gene            complement(19421..20680)
                     /gene="ylbE"
                     /locus_tag="ACHK3A_001544"
     CDS             complement(19421..20680)
                     /gene="ylbE"
                     /locus_tag="ACHK3A_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO8739646.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLDQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPHIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIFLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(20690..22357)
                     /gene="fdrA"
                     /locus_tag="ACHK3A_001545"
     CDS             complement(20690..22357)
                     /gene="fdrA"
                     /locus_tag="ACHK3A_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO8739647.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSASSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVIPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQPHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLVRLLERLQ"
     gene            22674..23723
                     /gene="allD"
                     /locus_tag="ACHK3A_001546"
     CDS             22674..23723
                     /gene="allD"
                     /locus_tag="ACHK3A_001546"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO8739648.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVNALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            23745..24980
                     /gene="allC"
                     /locus_tag="ACHK3A_001547"
     CDS             23745..24980
                     /gene="allC"
                     /locus_tag="ACHK3A_001547"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO8739649.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKQNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            24991..25776
                     /gene="allE"
                     /locus_tag="ACHK3A_001548"
     CDS             24991..25776
                     /gene="allE"
                     /locus_tag="ACHK3A_001548"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO8739650.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELEHIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(25905..27050)
                     /gene="glxK"
                     /locus_tag="ACHK3A_001549"
     CDS             complement(25905..27050)
                     /gene="glxK"
                     /locus_tag="ACHK3A_001549"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO8739651.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDAYGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(27072..28373)
                     /gene="ybbY"
                     /locus_tag="ACHK3A_001550"
     CDS             complement(27072..28373)
                     /gene="ybbY"
                     /locus_tag="ACHK3A_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO8739652.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTSDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(28430..29791)
                     /gene="allB"
                     /locus_tag="ACHK3A_001551"
     CDS             complement(28430..29791)
                     /gene="allB"
                     /locus_tag="ACHK3A_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO8739653.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(29852..31306)
                     /gene="ybbW"
                     /locus_tag="ACHK3A_001552"
     CDS             complement(29852..31306)
                     /gene="ybbW"
                     /locus_tag="ACHK3A_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO8739654.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLVICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFMVMRGQINLDELYTAAGDFKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTAAEKTGEQKVTG"
     gene            complement(31476..32354)
                     /gene="glxR"
                     /locus_tag="ACHK3A_001553"
     CDS             complement(31476..32354)
                     /gene="glxR"
                     /locus_tag="ACHK3A_001553"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO8739655.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(32454..33230)
                     /gene="hyi"
                     /locus_tag="ACHK3A_001554"
     CDS             complement(32454..33230)
                     /gene="hyi"
                     /locus_tag="ACHK3A_001554"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO8739656.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHFTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(33243..35024)
                     /gene="gcl"
                     /locus_tag="ACHK3A_001555"
     CDS             complement(33243..35024)
                     /gene="gcl"
                     /locus_tag="ACHK3A_001555"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO8739657.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMSVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTEACFMHYE"
     gene            complement(35114..35929)
                     /gene="allR"
                     /locus_tag="ACHK3A_001556"
     CDS             complement(35114..35929)
                     /gene="allR"
                     /locus_tag="ACHK3A_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO8739658.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(36007..36489)
                     /gene="allA"
                     /locus_tag="ACHK3A_001557"
     CDS             complement(36007..36489)
                     /gene="allA"
                     /locus_tag="ACHK3A_001557"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO8739659.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            36719..37645
                     /gene="allS"
                     /locus_tag="ACHK3A_001558"
     CDS             36719..37645
                     /gene="allS"
                     /locus_tag="ACHK3A_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO8739660.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAIEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            37714..38808
                     /gene="mnmH"
                     /locus_tag="ACHK3A_001559"
     CDS             37714..38808
                     /gene="mnmH"
                     /locus_tag="ACHK3A_001559"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO8739661.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVKGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTR"
     gene            38924..39289
                     /gene="ylbG"
                     /locus_tag="ACHK3A_001560"
                     /pseudo
     CDS             38924..39289
                     /gene="ylbG"
                     /locus_tag="ACHK3A_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(40056..40219)
                     /locus_tag="ACHK3A_001561"
                     /pseudo
     CDS             complement(40056..40219)
                     /locus_tag="ACHK3A_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10258 a        10498 c         9757 g         9706 t
ORIGIN      
        1 tatagcggta acttcaggcg cgacagacaa tgcaacgatt aaattattaa ttttcatctg
       61 aagctcaata atttttaact ctaaagtttc attagcatct ttcttgtttt caactggttg
      121 aattttgcta caattaaaaa gcaattcatt aatgatatta taatcaacca aatctctttt
      181 tattgatggc ctgtcacatc gatgtaatct tctcatcgga caaacataat agccatgcaa
      241 acttccagat accgcatgaa caatcatggt attaccacaa gcctcacact tcataactgt
      301 tcgaagtaga tttattagca taggattctt gctactattg ctaataccaa aaggtgccaa
      361 ccgaatttcc tgtacagcgt aaaacaaatc atctgatatg actctgggat aatagccagc
      421 gatttcactt atccctttcc ctcttgcacg atatgaaggt acgcatatac ctatcagagc
      481 tttattcgct aataattttt caattacaga aggtccccat gcactttctt ttcctgagaa
      541 attctttaca gcatgatcat ttaaatactt ggctattgca ttcaatgagc gcctttccat
      601 cctgagttta aaaattagct caatagtttt caccctgtcg gggtctggaa caaaagccgt
      661 tcttttgtca tctaaggaga gccatctcgg acaagacgcc gtcataatcg tacctgattc
      721 cagtgcatcc tgccgttttt tcttccatga taatttaacc cgacttgact ttatctcgct
      781 ttcttcattt gccctttgtg ctataagtat ggcttttatt aatgaatatg gctcattcaa
      841 agagtcaata ttatagactg tattatcgca aagagttata acatcaatac cgtgattcaa
      901 aatcaatttc agacgttcaa tcgcttcacc gactttttct cttgaaagtc tgtccagact
      961 ttcaactaac aatgtagttc ctggcaatat ataaccatgc tctatagcat ctaaaaattc
     1021 cgaaaaagct cctgattgtg catgctttcc tttgaatgca cttaatccta aatcttcata
     1081 tgttatggta tcaagataat aatcactatt tacctttaac cattcagcaa taagtcttct
     1141 ctgtcggttt aatgagtcgc cagacatctg acctggtgat gaaaatcgca tatatgctat
     1201 ggcttttttc atggtgacac ctgctaacgt atgcttttat aaaccttagt ggtgggatat
     1261 aatttttgtt taatttttat ttaaaaagac aattaaggtc acattatctt gaatatacaa
     1321 caataatcgt attgcaattt tcttacgcca taatcttgaa agcacaaaag aatacataaa
     1381 aaataaagac attaacaaaa agcataaaac gaggctcata taaatataag agcctccata
     1441 ttttagtcgt ttagaaacaa attattttaa tgtggtgtgc ttcgtgacaa taaattaata
     1501 accaacacac cggcacaaat caacatcatg cctataatgg ctggcaggtc cagccgttgg
     1561 ccgaaaaatc cccatgacag taagctaatc aggacaatac cgactcctga ccagatagca
     1621 taagcaatcc ctgtaggaat ataagccagc gtctgagcta ataaccagaa tgatgcacaa
     1681 taacaaataa ttgtaccaac agatggccat aaccgtgtaa aaccttctga aaacttcatt
     1741 aaggttgtac caatgacctc tgcaagtatt gcaccaccaa gataaatata agggttcata
     1801 gcatattctt tcctgttcaa actggagaga attgtactac agtttgaact caactcacct
     1861 gtttcatcat tgtgttccca ttgatgttct tttatatacc ctcagtaccc gtttcatcgc
     1921 ggcactctgg cgacactcct taaaaatcag attcgtgctc acctttcctt cccgttcttc
     1981 tctggtagcg aaccggtaat acaccgttcg ccagacctta ccatcaacga ccaggattcc
     2041 tgcccgcgcc attttagccg cagcctgatt tatgctggtt acggttgcgc ctgttaccgc
     2101 ggcaacgtcc tgtgcacaga agctcttatg cgtccccagg taatgaataa ttgcctcttt
     2161 gcccgtcata cacttgctcc tttcagtccg aacttagctt tgatttctgc gatcttcgcc
     2221 agagcctgtg cacgatttag aggtctaccg cccatgacag gaagttgttt tactggttca
     2281 ggtatcgcct caccacggtt aattcgcgcg gtcatacagg acagttcatc ggcagccttg
     2341 cgccgtaatt ccgcgtcagt caacgcattg gcccgcatgt tctgatacag gttggtaacc
     2401 agccagtagt gcgcgtttga tttccacgga taagactctg catccggata cagaccacgc
     2461 ttccggcaat actcgtaaac catatcaacc agctcgctga cgtttggcag cccggcggta
     2521 acggatgctt cttcccggca ccatgcaaca aactgcccgg gtgatggcag aaatggtcta
     2581 ttctgccgac gggcaacacg cattccggcg ctcacctgtt ccatcgtggt gatcccgttt
     2641 tcccggaaag ccagaaccca ctggcggcgg atttcgttca gttcgttctg gtcacgatta
     2701 gccaggctcg ccgggaaagt tgccagtaac tggctgaata caccgttgat tatctgcgct
     2761 acctgctgta cctgcggctt ttcgtcgtac tgttccggca tgttgttggc gatccgacgc
     2821 atctgctcac ggtcaaagtt aatcatctgt gcggcgatgt ttttcataga tccaccccgt
     2881 aaatccagtc tgtgtttgtc aggtcgagtt ttggtttgct agctgtcacg cctgcctgtt
     2941 gcttgttacg gttgatttcg agttgggtcc acttgtcgcg gagtttggcc ggacttagca
     3001 cgttaccgga ccagaagttg tcctggcatg cccagcggaa cagcacgcac atgtcgcggt
     3061 ggttacgtcc gtcacgttca cgcatcaggc ggatatcgtt agcccaccct gcaaaattcg
     3121 gttttctggc tgatggcgcg atggtcttca ccatgtcaaa catccactct gcggcggtca
     3181 ggtcttctgc tgtcccccac ttgctgccgc tctgaattgc agcatccggt ttcaccacag
     3241 gaagatcgtt ttctggttgg tcagaggatt cgccagaatt ctcggacgaa aaaggtttta
     3301 tattgtcttt tgttagtttg tcttttgtgt ttacctgatt cgggtaaacg cctttacctg
     3361 atttgggtaa acttttttta cctgattcag gtaaatttac ctctttcagg taaactttat
     3421 ttttcttacc tgattcgggt aatgttgacc attcactgac cacattatta atgccggtat
     3481 tccgcccgct ctgaataaaa atcccacgct ttaccagaac actttttgca gcagaacact
     3541 tgtgcggcaa tatcccggtt aattcggaaa gttgctcgtt gctaacccaa tccagttttt
     3601 tattaaagcc atatgttttg cgcatgacag ccagaaagac cagaagctgg tgctgtgtta
     3661 atccggccag catcacagct tccagcaact catttgcaat gcgcgtataa ccatcatcga
     3721 gatctgccac gcgcggctcc ttttgtgccg catccggcac tggaaaattg aatatctcag
     3781 cagtgtttgc cataattcct cccgcaatga gtgtgttacg atttgcacct gaaagtcggt
     3841 tctgttccag cagaccggct ttcgccattt ctgaacctgt catatcgccc ccagcatggt
     3901 agtaaccatc gccatcaatg gaccagccag atctgggtcc acacgaaaca tcgacacaat
     3961 accttcacta atttccttca gtttctggtg gcgtggtgcg ttgagaatga cagcctgttt
     4021 tgcctcactg agttcctttt ccatttcagc caacctagcc atgaagctat cctgctcaac
     4081 caggtaaccg cgatattcca gcggtagtac cgccagaatt gccggggtca gttcacgcac
     4141 gttatttcgg tatttttcag aatcgaattt gttatcgagg aagcggaaca gcttctggcg
     4201 tgcacggctg acatcatcag ggaaatcgat ggtgccgccg ccctgctccc gatactcatt
     4261 cacaatgagt gtggcaacga catcctgatt atctacagcc gaccaggcgc ggacggcatc
     4321 acggattttt tcgtggcctg gcacctgttt tgtttgagaa cgatttatca ccgcagtcgg
     4381 gctaaatccg ctagtctgtt ggtatgtaag tggttgcata attgactcct ttagtttgaa
     4441 ttgactgtta agttgattgc ttattgttaa agagcgtgaa atggaaattt aagctgcgtt
     4501 cttttcggtg tgtggaaaca acttcggaag atccgggcga atctggtatg ccttcactac
     4561 tccaccagta gccgtaacaa tgctgccgac atgttcaggg gatacctttg ctttgttgtg
     4621 aagccactta tagacggcct gctgtgaaac ttcgcaagca gcgcccagtt tcttttgtga
     4681 accaacgata ttgatcgctg ttttgatagc tgggttcata acaacctccg tggttaattt
     4741 gaatcaagat taaaactatg gttgttttta gtcaacaacc attttcgttt gatggaataa
     4801 aaccttggtt gtacatttgg actatgaaaa caacactctc agaaagactt aaagaagcca
     4861 gattagcgcg aggccttaca caaaaggcgc ttggggattt ggtcggggtt agccaggctg
     4921 ctattcagaa aatcgaaaca gggaaagcta atcaaacaac taaaatcgtg gagatcgcga
     4981 acgctttggg tgtgcgcgca gaatggttat cttctggcgt tggaaatatg tcagacagta
     5041 cagtgcaacc aatacaatca actgtcagcc attccaaata cttcaagatt gacgttcttg
     5101 atatagaagt cagtgctggg ccgggagtca tcaaccgtga gtttgtagaa gttctacgct
     5161 cggttgagta ctcgtttgac gatgctcgtc acatgttcga tggtaggaag gcggaaaata
     5221 tccgcatcat taacgtgcgt ggtgacagca tgtcaggaac gatcgaacca ggtgatctgc
     5281 tgttcgttga tatcacagtt aaatctttcg acggtgatgg tatctatgcg tttctgtacg
     5341 acgacacagc ccatgtaaag cgcctgcaaa tgatgaagga taagctgctg gtcatctctg
     5401 ataacaaaag ctactcaccg tgggacccga tcgagaaaga cgagatgaac cgggtgttca
     5461 tcttcggtaa ggttattggg agcatgccgc agacatatag gaagcatggg tagtaccaat
     5521 taaaaattat caactgggca ttgtgctcat tcagtaaaga actaattcct atctttgctc
     5581 taggtagtaa tattaagcca ccgcaataat atctttacct aacggcgtaa gaatcccggt
     5641 caccgtgccg ggttttcttt tgccctcccc tcatcacaca caccgttaaa aaaaccacca
     5701 taacctcgct tcagttatcg ctatgcgatt caagtcacaa aataaatcca tcctaaatac
     5761 aaccagttat atctaaaaca accaataaaa caacttttgt tgttgacgat aaaacaacta
     5821 tagttttaaa taagttcatc gcaacaacac aacgatacgg caactacctg attcaccgtt
     5881 gcgatgaccg cttagatccg cagtttgaat ttcagcaggc ttcggggagt gcgaggggtg
     5941 aaacggacgc gtgaacgtcg gtgtgaccag ctgaaattaa ctcaacattt cataccttag
     6001 tcgcttcaac gaggcggctt agttatgaca accggcggcc atccaccgcc tgaatacgcg
     6061 cagaagtctc tatatgttca gcagcccagc ttacgggcag gagtttttat ggttcatcaa
     6121 cattatggaa cgcagaccgt taatcgaggt gcggtcatgc caggaatgct ggtcaaacac
     6181 aaagatggta cctggactgc atcagctaat ttacgcggac ggctttatct gcatcgcggc
     6241 atcgagcgca cttatacccg tgatttgctc gtggaagttt ttctcgacgg acgcggtaac
     6301 ggcctgaatc actaatcccc tttcctgttt tcctaatcag cctggcattt cgcgggcgat
     6361 attttcacag ccattttcag gaggtcagcc atgaacgctt attacattca ggatcgtctt
     6421 gaggctcaga gctgggcgcg tcactaccag cagatcgccc gtgaagagaa agaggcagaa
     6481 ctcgcagacg acatggaaaa aggcctgccc cagcacctgt ttgaatcgct atgcatcgat
     6541 catttgcaac gccacggggc cagcaaaaaa gccattaccc gtgcgtttga tgacgatgtt
     6601 gagtttcagg agcgcatggc agaacacatc cagtacatgg ttgaaaccat tgctcaccac
     6661 caggttgata ttgattcaga ggtataaaac ggatgagtac agcactcgca acgctggcag
     6721 ggaagctggc tgaacgtgtc ggcatggatt ctgtcgaccc acaggaactg atcaccactc
     6781 ttcgccagac ggcatttaaa ggtgatgcca gcgatgcgca gttcatcgca ttgctgatcg
     6841 tcgccaacca gtacggcctt aatccgtgga cgaaagaaat ttacgccttc cctgataagc
     6901 agaacggcat tgttccggtg gtgggcgttg atggctggtc ccgcatcatc aatgaaaacc
     6961 agcagtttga tggcatggac tttgagcagg acaatgaatc ctgtacatgc cggatttacc
     7021 gcaaggaccg taatcatccg atctgcgtta ccgaatggat ggatgaatgc cgccgcgaac
     7081 cattcaaaac ccgcgaaggc agagaaatca cggggccgtg gcagtcgcat cccaaacgga
     7141 tgttacggca taaagccatg attcagtgtg cccgtctggc cttcggattt gctggtatct
     7201 atgacaagga tgaagccgag cgcattgtcg aaaatactgc atacactgca gaacgtcagc
     7261 cagaacgcga catcactccg gttaacgatg aaaccatgca ggagattaac actctgctga
     7321 tcgccctgga taaaacatgg gatgacgact tattgccgct ctgttcccag atatttcgcc
     7381 gcgacattca cgcatcgtca gaactgacac aggccgaagc agtgaaagct cttggattcc
     7441 tgaaacagaa agccactgag cagaaggtgg cagcatgata ccggacatta tcctgcagcg
     7501 taccgggatc gacgtgagag ctgtcgaaca gggggatgat gcatggcaca aattacggct
     7561 cggcgtcatc accgcttcag aagttcacaa cgtgatagca aagccccgct caggaaagaa
     7621 gtggcctgac atgaaaatgt cctacttcca caccctgctg gctgaggttt gcaccggtgt
     7681 ggctccggaa gttaatgcta aggcgctggc ctggggaaaa cagtacgaga acgacgccag
     7741 aaccctgttt gaattcactt ccggcgtgaa tattactgaa tccccgatca tctatcgcga
     7801 cgaaagtatg cgcaccgcct gctcccccga tggtttatgc agtgacggca acggccttga
     7861 actgaaatgc ccgtttacct cccgggattt catgaaattc cggctcggtg gtttcgaggc
     7921 aataaaatcg gcttacatgg cccaggtgca gtacagcatg tgggtgacgc gaaaagatgc
     7981 ctggtacttt gccaactatg acccgcgcat gaagcgtgaa ggcctgcatt atgtcgtgat
     8041 tgagcggaat gaaaagtaca tggcgagttt tgacgagatg gtgccggagt tcatcgaaaa
     8101 aatggacgag gcactggctg aaattggttt tgtatttggg gagcaatggc gatgacgcat
     8161 cctcacgata atatccgggt aggcgcgatc actttcgtct actccgttac aaagcgaggc
     8221 tgggtatttc ccggcctttc tgttatcaga aatccactga aagcacagcg gctggctgag
     8281 aagataaata ataaacggga ggcggtatgc acaaagcatc tcctgttgag ttaagaacga
     8341 gtattgaggt ggcacatagc cttgctcaaa ttggagtcag gtttgtgcca ataccagtag
     8401 aaacagacga agaatttcat acgttagcca catccctttc acaaaagctg gaaatgatgg
     8461 tggcgaaagc agaagcagat gagagagacc aggtatgaca accactgaat gcatttttct
     8521 ggcagcgggc ttcatattct gtgtgcttat gcttgccgac atggggcttg ttcaatgaca
     8581 cctcagcaag aaaacgccct tcgcagcatt gcccgtcagg ctaattctga aatcaaaaaa
     8641 gccagacagc attttccgga taaaaacgtc gatgacattt gccgtagcgt actaaagaag
     8701 caccgcgaaa cggtaacgct gatgggattc acaccgactc atttaagcct ggcgatcggc
     8761 atgttgaacg gcgtctttaa ggaacggtaa acatgaaaag caaaatcatc agggagctac
     8821 aggctccttt tttattattc gcatttaccc tcaagcgtat taaccaacaa ttcagggatt
     8881 aatgaaagat ggcagacatc attgattcag catcagaaat cgaagaatta cagcgcaata
     8941 cagcaataaa aatgcgtcgt ctgaactacc agactgtatc cgcaactcat tgttgtgagt
     9001 gtggcgatcc gatagatgag cgaagacgcc tggctgttca gggttgtcgg acttgtgcga
     9061 gttgccagga ggagatcgaa cttaagaaca aacaataggg actgtgatgg cctcaaagca
     9121 gcaaatttca acatcgtcca actgaggtgt aaaaatgttc agaatcattt ttcctaacac
     9181 ctggtacgtc gaccaccacg gcactccctg caaaatcctg cgttctaccc acaacaaagt
     9241 tcactacatc cgaaaaggca gaacatgtat cgccagcatg ttccgcttta atcatgactt
     9301 tgaacctgtg aataaagctg atgcagatcg gatagcagaa gagatcgaaa cggcagaaca
     9361 cattaagaag ttacgtgaca tgcgttcaaa aagcagaggt aaccatggaa tcatacagcc
     9421 tcacactcga tgaggcctgt cagtttctta agatatccag accaaccgcc accaactgga
     9481 tacgaacagg ccgcctacag gcaacacgta aagatccaac caagccaaaa tctccttacc
     9541 tcacaacacg gcaagcctgc attgcggcgc ttcagtctcc gctgcatact gtccaggtga
     9601 gcgcgggtga tggcataaca gaggaaagaa aatgtcactc ttccgcagaa atgaaatatg
     9661 gtatgcctcg tattcgctcc cgggcgagaa acgaattaag gaatctcttg gcacaaagga
     9721 caagcggcaa gctcaggagt tgcacgacaa gcgaaaagca gaactctggc gagtagaaaa
     9781 gctaggggat ttacctgatg tcacttttga agaggcctgc ctaagatggc ttgaggaaaa
     9841 agctgataaa aaatctctcg attcagataa aagccggatt gagttctggc ttgaacattt
     9901 tgagggtata aggcttaaag atatctcgga ggcaaagatt tactctgctg taagcagaat
     9961 gcataacaga aagacgaaag aaatatggaa acagaaagtt caggccgcca tcaggaaagg
    10021 taaagaaccg cctgtttatg aaccaaagcc agtatcaact cagacaaagg caaagcatct
    10081 tgccatgata aaggccattc tccgtgctgc agaacgcgac tggaagtggc tggaaaaagc
    10141 gcctgtcatc aagataccag cggtcagaaa caagcgagtc agatggctgg aaaaggagga
    10201 agcaaaacgc cttattgatg agtgccccga accactgaaa tctgtcgtca agtttgcgct
    10261 ggcaactggt ctgagaaagt cgaacatcat aaatctggaa tggcaacaaa tcgacatgca
    10321 gcgacgagtt gcctgggtga atccagaaga gagcaaatca aaccgcgcca ttggtgtggc
    10381 gctgaacgat accgcctgta aagtgttgcg tgatcaaata ggcaagcatc acaaatgggt
    10441 gtttgtacat accaaggcgg ctaagcgagc agatggaaca tcaacgcctg cggtcaggaa
    10501 gatgcgcatc gacagcaaga catcatggct atcagcttgt cgtcgtgcag gaattgaaga
    10561 tttccgtttc catgacctca gacacacctg ggcaagctgg ctgattcagt caggcgtccc
    10621 attatcagtg cttcaggaaa tgggcggatg ggagtccata gaaatggttc gtaggtatgc
    10681 tcaccttgcg cctaatcatt tgacagagca tgcgaggaaa atagacgaca tttttggtga
    10741 taatgtccca aatatgtccc actctggaat tatggaggat ataaagaagg cgtaactgat
    10801 tgaattgtaa tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt
    10861 tgctctatcc aactgagcta agggcgcatt gataccacgg tgtgctgtaa tcgcgtgaat
    10921 tatacggtca acccttgctg agtcaatggc ttttgaacca gttgctgaac aagtgtacga
    10981 ccgcatcaga tgtagctcac tatggcggca aaccaacgac ggttatcatg ataggtgtga
    11041 atcagccagg tacagaaggt tttacttttc agaaaacgaa aactgacagc gcgccacgct
    11101 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
    11161 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
    11221 aaagttcagg cgcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
    11281 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
    11341 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
    11401 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
    11461 ccggcgggta ttgataacgt caaagtgctg gaacgtatac acccggacaa agacgtggac
    11521 ggtttccatc cttataacgt cggtcgcctg tgccagcgcg cgccgcgtct gcgtccctgc
    11581 accccgcgcg gtattgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
    11641 gccgtggtga ttggcgcatc gaatatcgtt ggccgcccga tgagcatgga actgctactg
    11701 gcaggttgca ccaccaccgt gactcaccgt ttcactaaaa atctgcgtca ccacgtagaa
    11761 aatgccgatc tactgatcgt tgccgtcggc aagccgggct ttattcctgg tgactggatc
    11821 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
    11881 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttccgggc
    11941 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
    12001 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
    12061 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
    12121 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
    12181 aatcgtcgcc gggcagacag tgagttttgc aggtcacagc gtacaggttg ttgcctgatc
    12241 cctttcccgg cctgtaacaa caggccggtt ctcttttcag acttttgccc tctacgcccc
    12301 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
    12361 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagt catccctccg
    12421 cgtctgttat ttgccggaat cgtcctggcg atgctgcccg atgccgacgt gttgtcgttt
    12481 aaatttggcg ttgcttacgg caatgttttt ggtcatcgcg ggtttaccca ttcgctggtg
    12541 tttgcgtttg ttgtcccgct gttatgtgtg ctgattgggc gtcgatggtt cagggctggg
    12601 ctgattcgct gctggctgtt tttaaccgtc tcgttgctat cgcacagctt gctggattcg
    12661 gtaaccactg gcggtaaagg ggttggctgg ctgtggccgt ggtcagatga acgctttttc
    12721 gctccctggc aggtgattaa agtcgcgccg tttgcattgt ctcgttacac cacgccgtac
    12781 gggcataagg tgattatttc tgaattgatg tgggtgtggt tgccggggat gctactgatg
    12841 ggaatgttgt ggtggcgcag acgataaccg gatgcgaaaa ctcgcatccg gcaatagcgc
    12901 aattacttac gacgccaggt ggtcccttgc gggccatctt ccagcacgat ccccatctcg
    12961 ttaagacggt cacgcgccgc atctgccgcc gcccagtctt tcgctttacg ggcatccaga
    13021 cgctgttgaa ttaacgcttc aatctcagcc acttcactgt cgtctgcctg cgcgccgctt
    13081 tgcaggaacg cttccggttc ttgctccagc aggcccaata cagcggaaag tttacgcagg
    13141 tgagacgcca ttgcattcgc cgctgccata tcttctgctt tcagacggtt cacttcacgc
    13201 gccatatcaa acagtacgga ataggcttcc ggggtgttga aatcgtcgtt catcgcctca
    13261 ataaagcgcg cttcaaacgc ttcgccaccg gcaggagcaa cggttttgtc tgtgccgcgc
    13321 aaggcagtat agagacgttc cagcgccgca cgcgcctgct tcaggttctc ttcgctgtag
    13381 ttcaactgac tgcgatagtg acccgacatc aggaagtaac gcacagtttc cgcatcgtag
    13441 tatttcagca catcacgcac ggtgaagaag ttacccagcg atttagacat cttctcgcgg
    13501 tcaaccatca ccatccccga gtgcatccag tagttcacat actgaccatc atgggcacag
    13561 gtggactggg caatttcgtt ctcgtggtgc gggaacatca ggtcagaacc gccgccgtgg
    13621 atatcaaagt ggttacccaa ctgtttgcag ttcattgccg aacactcaat gtgccagcca
    13681 ggacggcccg cgccccacgg agacggccag ctcggttcgc cctctttcga catcttccac
    13741 agaacgaaat ccatcgggtt gcgtttgtcg tcgaccacgt caacgcgcgc gcctgcctgc
    13801 agctgatcga gatcctgacg cgacagcacg ccataagttg gatcggtcgg gacgtcgaac
    13861 atcacgtcgc cgttgtccgc cacataagcg tgacctttgg cgatcagttg ttcagtgagt
    13921 tcaataattt cggcgatatg gtgcgtcgcg cgcggctcca tatccgggcg cagaatgttc
    13981 aaagcatcaa aatctttgtg catttcggcg atcatgcgat ccaccagcgc cacaaagctt
    14041 tcgccatttt cattggcgcg tttgatgatt ttgtcgtcga tatcagtaat gttgcgcaca
    14101 tacttcagct tatagccgag gaaacgcaga tagcgcgcaa ccacgtcaaa ggcaacaaag
    14161 gtacgcccgt gaccgatatg acagagatcg taaacggtga ttccacacac gtacatgccg
    14221 acttccccgg cgtgaatagg cttaaattcc tctttttggc gtgtcagagt attgaagatt
    14281 tttagcatcg aagattccgt ttagacatgt gtgggtaatt gagttgcgta taatacccat
    14341 atttcccgcg cgaatcagca tacattgcgt gatgatccga tcgtgcggtt atgctataac
    14401 accaccctat atatgacccg aaccgggttg aagcaccaat caaacggaac aggatgcaaa
    14461 aatggttact ttccacacca atcacggcga tattgtcatc aaaacttttg acgataaagc
    14521 acctgaaaca gttaaaaact tcctggacta ctgccgcgaa ggtttttaca acaacaccat
    14581 tttccaccgt gtaatcaacg gctttatgat tcagggcggc ggttttgaac cgggcatgaa
    14641 gcaaaaaacc accaaagacc cgatcaaaaa cgaagctaac aacggtctga aaaatacccg
    14701 tggtacactg gcaatggccc gtactcaggc accgcactct gcaactgcac agttcttcat
    14761 caacgtggtt gataacgact tcctgaactt ctctggcgaa agcctgcaag gttggggtta
    14821 ctgcgtgttt gctgaagtgg ttgaaggcat ggacgtggta gacaaaatca aaggtgttgc
    14881 aaccggtcgt agcggtatgc accaggacgt gccaaaagaa gacgttatca ttgaaagcgt
    14941 gaccgttagc gagtaatcgt ggcgacactc tttattgcag atcttcatct ctgcgcggaa
    15001 gaaccggcga tcaccgccgg ttttctgcgt tttttagcgg gggaagcccg caaggccgac
    15061 gcgctgtata ttcttggcga tctgtttgaa gcatggattg gcgacgacga tcccaaccca
    15121 ctccatcgcc agatggcggc agcgatcaaa gcggtgtccg attccggcgt tccctgttat
    15181 ttcattcatg gcaaccgtga ttttctgctc ggcaaacgct ttgcccgtga aagcggcatg
    15241 acgttattgc cggaagaaaa ggtgctcgaa ctttatggtc gccgggtgtt gattatgcat
    15301 ggcgacacgc tgtgcaccga tgacgccggt tatcaggctt ttcgcgccaa agtccacaaa
    15361 ccctggctgc aaacgctatt cctcgccctg ccgttgtttg tgcgcaaacg cattgccgcg
    15421 cgaatgcgcg cgaacagcaa agaagccaac agcagtaaat cgctggcgat catggacgtt
    15481 aaccaaaacg cggtggtcag tgcgatggaa aaacatcagg tgcaatggct gattcacggg
    15541 catacccatc gcccggcagt acatgaactt atcgccaatc agcaacctgc tttccgcgtc
    15601 gtgctgggtg cctggcatac tgaaggttcg atggtgaaag tcacggcgga tgacgttgag
    15661 ttgatccatt ttccatttta aaaaacgcgc aactttgctg atttcataac cgcgcaaccg
    15721 ttttccttgc tctctttccg tgctattctc tgtgccctct aaagccgaga gttgtgcacc
    15781 acaggagttt taagacgcat gtcttcccgc aataatccgg cgcgtgtcgc catcgtgatg
    15841 gggtccaaaa gcgactgggc taccatgcag ttcgccgccg aaatcttcga aatcctgaat
    15901 gtcccacacc acgttgaagt ggtttctgct caccgcaccc ccgataaact gttcagcttc
    15961 gccgaaagtg ccgaagagaa cggttatcag gtgattattg cgggcgcagg cggcgcagcg
    16021 cacctgccag gcatgattgc cgccaaaacg ctggtgccag tgctgggcgt gccagtacag
    16081 agcgccgcac tgagcggtgt cgatagcctc tactccatcg tacaaatgcc gcgcggcatt
    16141 ccggtgggta cgctggcgat tggtaaagct ggcgcggcaa acgcggcgtt actggcagca
    16201 caaattcttg cgactcatga taaagaactg caccagcgtc tgaatgactg gcgcaaagcc
    16261 cagaccgacg aagtgctgga aaacccggac ccgcgaggtg cggcatgaaa caggtttgcg
    16321 tcctcggtaa cgggcagtta ggccgtatgc tgcgtcaggc aggcgaaccg ttaggcattg
    16381 ctgtctggcc ggtcgggctg gacgctgaac cggcggcggt gcctgttcaa caaagtgtga
    16441 ttaccgctga gatcgaacgc tggccggaaa ccgcattaac ccgcgagctg gcgcgccatc
    16501 cggcctttgt gaaccgcgat gtgttcccga ttattgccga ccgtctgact cagaagcagc
    16561 ttttcgataa gctccacctg ccgaccgcgc cgtggcagtt acttgccgat cgcagcgagt
    16621 ggcctgcggt gtttgatcgt ttaggtgaac tggcgattgt taagcgtcgc actggtggct
    16681 atgacggtcg cggtcaatgg cgtttacgcg caaatgaaac cgaacagtta ccggcagagt
    16741 gttacggcga atgtattgtc gagcagggca ttaacttctc tggtgaagtg tcgctggttg
    16801 gcgcgcgcgg ttttgatggc agcaccgtgt tttatccgct gacgcataac ctgcatcagg
    16861 acggtatttt gcgcaccagc gtcgctttcc cgcaggccaa tgcgcagcag caagcgcaag
    16921 ccgaagagat gctgtcggcg attatgcagg agctgggcta tgtaggcgtg atggcaatgg
    16981 agtgttttgt caccccgcaa ggtctgctga tcaacgaact ggctccgcgt gtgcataaca
    17041 gcggtcactg gacacaaaac ggtgccagca tcagccagtt tgagctgcat ctgcgggcga
    17101 ttaccgatct gccgttaccg caaccggtag tgaatagtcc gtcggtgatg atcaacctga
    17161 ttggtagcga tgtgaattat gactggctga aactgccgct ggtgcatctg cactggtacg
    17221 acaaagaagt ccgtccgggg cgtaaagtgg ggcatctgaa tttgaccgac agcgacacat
    17281 cgcgtctgac cgcgacgctg gaagccttaa tcccgctgct gccgccagag tatgccagcg
    17341 gcgtgatgtg ggcgcagagc aagttcagtt aattgatgct cattttcttg ccggatgcgg
    17401 cgtgaacgcc ttatccggcc caccgctcca acaccgactg taggcctgat aagacgcgcc
    17461 agcatcgcat caggcatggt gtgccgattg caggatgcgg cgtgaacgcc ttatccggcc
    17521 taccgttcca acaccgactg taggcctgat aagatgcgcc agcatcgcat caggcatggt
    17581 gtgccgattg ccggatgcgg cgtgaacgcc ttatccggcc taccgttccg acaccgcctg
    17641 tagacctgat aagacgcgcc agcatcgcat caggctagat gtgccaattg cctggtgccc
    17701 atcactacag cgaaatacaa gtccccgctt cgcccgccag cgtctcttca atccgcgata
    17761 acgccccaat ccacgcgggt ttgccacggc ttctgacata gccactcacc gcagtcacct
    17821 tcggccccat cgaaccatcg gctttggcaa atggcgctaa ctcatccggt gtggcatggc
    17881 gaatggcacg ttgctgcggc gttccccagt tttcatatac cgcatcagca tcggtgagga
    17941 tcaccagtcc atcggcatta atctgctcgg cgagcaacgc agcggcgaga tctttatcaa
    18001 ttaccgcttc actccctgcc ccgtctttcg tcacaggcac accgccgccg ccactgcaaa
    18061 tcaccacatg cccctctttg agcaacaact cgatggcttc gctgtcgaga attttacgcg
    18121 gttgcggaga cgccaccacc cggcgtaaat atttaccatc acgtttcatc tgccagccat
    18181 aagccgcttc cagtgcctct tgttcttctg gctgataaac cggaccgata aacttctctg
    18241 gctgcaaaaa cgccggatca tcaggcgaaa cttcaatgcg cgtcagcact gtcgtgacag
    18301 gcggcatctg cggctgtgcg ctcaagctct gcgccagcat atagccaatc atcccctggc
    18361 tttccgccac cagcacatcc agcggatacg gttctacctc tttccacgcc agattctgta
    18421 acgccagcag ccccacctgc ggcccgttgc cgtgaacaat cgccagccga taagaacggg
    18481 ccaggcgcgc cagtgcgggt acagcactgg caatgttgcg atattgattt tctgccgtca
    18541 gcgcctcacc gcgctggagt aaggcgttgc ccccaagagc cacaaccagt gttttcatga
    18601 ttttccttgt aataattgtt ggccaagcca gaaccccagc agcgtatctg cccctgacgt
    18661 atgccccagc gccagcagcg aatcaatcgc aacagcggta cgtttcgggc aactcagagc
    18721 atgaataaag tgcaggagtg gcgaggcgaa gtatccttgc gcggcataac gtaaataact
    18781 gacgctcacc gctgtggtaa cgagttgaag attgtcggaa caggcaaaaa acgggcgacc
    18841 ggagcgcgca tctaaagcgc cataatacca ggccgccagc agcattccgc tcagcgtgtc
    18901 atcatgactt ggcgttaatc ctgggccttt acccagccag tgccgccagt cggtcttaac
    18961 gccattgagc gcggcctgaa aacagtgacg aaactggcgt aattcagcag gaagcggatc
    19021 gcctgccgcc aacgccagtg gcccgaaaag cccggtttcc tccgcgcgtt gcatccatgc
    19081 cgctgcaagc ggttgaggat gcgcaggcgg cgtaatacgc agcaaacaat aacgctgtgg
    19141 ctgtttaacc gtgaaacgtc cgaggcgaat cccttgagcc ataacctgtg gtcgttcatt
    19201 gccgcataat ccaccgcata acgcatcgaa ttgcgcgcgg cgaagcaccc atcctccggg
    19261 gccgaaacca ctaccctgac gatgcagcgt taacagttcg ccgctttccg tcatcaggtt
    19321 aatcgcccgc cgccacactc ccgctaaccg ccaggactgg cgataattcg gtgcgctact
    19381 actggcaagc agaggatgga tgatcgtcat tgtgcgttcc tcaaccaatc cccatgcttt
    19441 cagccagtgc ttccagcgcc tgctcaaagc acgccagcgg tgcccgcacg gtgcctgcgc
    19501 caatctgccc gatccccgcc tctttatggg cgattccggt attgatgagc ggcgtaatgc
    19561 cggtttctac cacgcgacga atatccagcc ccaggcacgc gccctggaaa tcccagccgg
    19621 ggatctgcaa ctgcatattg cgttcaagga agatttccgc catctcttca gataccgctc
    19681 tggccgcttc catgccaccc gcaccgacaa agcgcgttac gccaggcgct gcgatcatcg
    19741 ccgcaccgcc aataccaaag gtttcggtaa ttgcgctatc gcccatatcc gggttcgcct
    19801 gctcctgcga gaagccggtg aaaaacagcc cttggggagt gtttacaggc gcagtaaacc
    19861 agcgttcgcc cagcccgctt acccgaatcc cgaacatatt gccgttgcgg gtcatagccg
    19921 tgacgatgct gcctgcgcgg atcatcgcac cagcatccat cgccgccttg cagtaagcca
    19981 tcgcgaggtt aaggaagaac tgatcggtca cgctgaggaa atccatcact tccgcgatgt
    20041 gctgtttatc atgatcgagg cgagcaatgt gtggggccag cgcacgcatt aacagtgctg
    20101 aggaagcaat attgcgttga tggaactcat cgcccatggt aatgccctgc gccatcatcg
    20161 cggtgagatc gataccgcgc tccatgcgcc ccagcgccgc gcttaatact ggcattaaca
    20221 catcgcgcat ccagcgatgg cgagtcagaa catcttcgcc gtaagcgcca aaacgcatca
    20281 ctttgccgat accttcgttg aggttgcagt acgcccggtt gccgtcggtc acgttctcga
    20341 ccaccagcat cggcatactg gcagaagtaa tgccgcccat tggcccgacg gcattcacat
    20401 ggtgacaagg aatgaagttc acttccccct gatccagcat tgccagcgcc tgcgcttcat
    20461 ctttcgccca gccttcaaac agacatgcgc ccacacatgc ccctttcatg ggtccggtca
    20521 tctcctgcca gcgcattggc ggcccggcgt gaagcagtgt tttgccctcg tttagttcgc
    20581 tgataagtga agaagccggt tgcacatcca gccagtgtgg acgagcgcga cgaatttgtt
    20641 cgataaccgc agcattggct tgcgccactg atgtaaacat gggaacccct tattgcaaac
    20701 gttctaataa acgaaccagt tttttattgc caccggcgac tggcgaccat tggtaatgca
    20761 caaccggttt gctggcgctt tgtagctcca gcgcaaagct gcgtaatccg atgttaatca
    20821 cggcgacgtt ttccagtaat gacggtgtat gtggctgtgt ggcaggcgag agcggatgaa
    20881 ttaacgccgc cgccagcaag gtggcttccg gcagcgaact cacgaccgca atccccgcat
    20941 cttccagcgt ggcgatttgc tgcgagcggc attgcgggtc acgttcagtg cctgtcaccg
    21001 tggcaatggc gtacagcggt tggctgtcag aacgcgcggc acaggctttt tgccaggcgc
    21061 tcaccagcga ggcggcagga tcggcggtcg caccgaagcc aatcacgaca tcaagcagca
    21121 acacgcgcac ttgcggttta gcgccgagat cggcaattaa caggttgcgt aaggtcggat
    21181 cgatcatcgg atggggacgc ccgacggtgt aaaaatcatc gcccaggtcg aggatctgat
    21241 gtccgtcggc gtccagcatc atgccatgtt gatgggtatc gtcggcttcc acgccaaggt
    21301 gtccggcaag taatcccgcc gcttcagcag ccagcgtacc gccggtatac aagccgcaaa
    21361 taaatccgct gctgacaggc gctattgcgt tacgtcgcgc agtgacgcgt gaaagcagac
    21421 aagccaggcg tgcggcctca tccagcgagg aggcaaacca gacattctcg tcgcgggcca
    21481 ccgccggggt ataacctaaa aacagcgcca ccgtcggttt gccagttgct ttcatggcat
    21541 taacaatttt cagacgcacc gcttcggcag gtggttttga aacaaatgcc agcacttcgc
    21601 ttttctcgtc tgcactgagc atttccagcg ccgtcagcgc actgatgccg cccacttcac
    21661 ggctgaggtc gcgcccgcca aggccaatcg cgtgagtaat tccctcccct gccagcgcaa
    21721 tctgtgagca cagctcctga atcccggtac cggaagcgcc aatgacgcca atgttgcctt
    21781 ccggtatcac gttagcaaaa gccagcggcg tgccggcaat catcgacgta ccgcagtccg
    21841 gccccatcac cagcaagcct ttttcccgcg cgcgggtttt aagttggatt tcatcttcca
    21901 gcgtgacgtt atcggagaac atcatcacgt tgaggttgcg atccagcgcc tggttcgcca
    21961 gctccgccgc atactcacca gccactgaaa tcagcgccag attggcatcg ggtaatttct
    22021 gacaggcact gtcccagcga cgcacctgcg tcaacgcctg gctgctggct gacccctgcg
    22081 ccagttgttt tagcgcctct tcaagctgct gcataatcgc ctgcgcgatc cccgcatccg
    22141 ccgcttcgct acgaattgcc acgcaaatat cgttcggcgt ggcgttatta aaatcgtcat
    22201 gccagaaacc tgtggtatct aataacgctt tattggcggg cgtacccatc attacggaaa
    22261 catcatcaac attttctgat tcgctgagtt ttcgtgaaat aatcattaaa ctgaccgaat
    22321 cctgaaaaca ccctttttta ataaaggcgt ggatcataac aactccttaa aaattgcacc
    22381 catggcaatt gcacgagcag tgttaatgtg gcgattacgg tatcgatgac aacgagtaaa
    22441 taaggtgatg aatatcacac agggattatt aacaaaagaa ttcaaataac cgccatgcta
    22501 ttacgtgtta ttttttaata agcgtggttg ttttaaaaac caggttaaaa gcgagcggat
    22561 tatcaaataa attctaacta tatttttttg cctgtctgga tcacataatc cagatatttt
    22621 ccctgtatgt taattgcagt catgcttcac accgtcgtta aaaaggaaga cagatgaaaa
    22681 tcagtcggga aacactccac cagctaattg agaataaact ctgccaggct gggttaaaac
    22741 gtgagcacgc tgcaaccgtg gctgaagtat tggtttacgc cgatgccaga gggatccact
    22801 ctcatggcgc ggtgcgcgtg gaatactacg cggaacgcat ttcaaaaggc ggcaccaacc
    22861 gcgaaccgga gtttcgtctt gaggaaaccg ggccgtgctc ggcaatttta catgccgaca
    22921 atgccgccgg acaggtcgcg gcgaaaatgg gtatggaaca tgccatcaaa accgcccagc
    22981 aaaatggcgt tgcggtggtc ggtatcagcc ggatgggtca cagcggcgca atctcttatt
    23041 ttgtgcagca ggcagctcgc gccgggttaa ttggcatttc gatgtgccag tccgatccaa
    23101 tggtggtgcc gtttggcggc gcggaaattt actacggtac taacccactg gcctttgccg
    23161 cgccgggaga aggcgacgag atccttacct ttgatatggc gactaccgta caggcatggg
    23221 gaaaagtgct cgacgcccgc tcgcgtaata tgtctatccc ggatacctgg gcggtcgata
    23281 aaaacggagc accaacaacc gatccatttg cagttaacgc cctgctccct gccgccggac
    23341 cgaaagggta tggcctgatg atgatgattg acgtcctctc aggcgtctta ctcggcttac
    23401 cgttcgggcg acaggttagt tcgatgtatg acgatttaca cgccgggcgt aatttggggc
    23461 aattacatat cgttattaat ccgaactttt tctcctccag cgaattattc cgtcaacatc
    23521 ttagccagac catgcgcgaa ttaaatgcca ttacccccgc gcccggtttt aatcaggttt
    23581 attatcccgg acaggatcag gatattaaac aacgcaaagc cgccgtcgaa ggcatcgaaa
    23641 ttgttgatga tatttaccag tatttaattt ccgacgcgct ttataacacg tcatacgaaa
    23701 cgaaaaatcc ctttgcgcaa taattattga gacaggactt tcttatgatt acacatttcc
    23761 gtcaagccat agaagaaacg ctgccctggc tttcctcttt tggcgctgac ccagcgggtg
    23821 ggatgacccg tttactttat tcgccggaat ggctggaaac tcagcagcag tttaaaaaaa
    23881 gaatggcagc aagcgggctg gaaacacgtt tcgatgaagt ggggaattta tacggtcgcc
    23941 tgagtggcac cgaatatcca caggaagtgg ttctgagcgg ttcgcatatc gataccgtgg
    24001 ttaacggcgg taacctcgac gggcaattcg gtgcgctggc ggcgtggctg gcaattgact
    24061 ggctgaaaac gcaatacggc gcgccgttac gtacggtcga agtggtggcg atggcagaag
    24121 aagaaggcag ccgcttcccg tatgtcttct ggggcagtaa aaatatcttt gggctggcga
    24181 atcctgacga cgtgcggaat atctgtgatg ccaaaggaaa tagttttgtc gatgcgatga
    24241 aggcttgcgg atttactctg ccgaacgccc cactaactcc gcgtcaggat attaaagcct
    24301 ttgtcgaact gcatatcgaa cagggctgtg tgctggaaag taatgggcag tcaattggcg
    24361 tggtgaatgc aattgtcggg cagcgtcgtt atacggtgac gctgaacggc gaatcaaacc
    24421 atgcaggcac cacgccgatg ggttatcgtc gtgatacggt ttacgctttc agtcgcattt
    24481 gccatcagtc gatcgaaaaa gcgaaaaaga tgggcgaccc gctggtgctt acctttggca
    24541 aggtggagcc gcgtccgaat acggtgaatg tggtaccggg taaaaccacg ttcaccattg
    24601 attgtcgtca taccgacgct gccgtgctgc gcgatttcac ccagcagtta gaaaacgaca
    24661 tgcgggcgat ttgcgatgaa atggacattg gtattgatat cgatttatgg atggacgaag
    24721 aacccgtgcc gatgaataag gagctggtcg ccaccctgac agaattgtgt gaaagtgaaa
    24781 aacagaacta ccgggtaatg cacagtggtg ccgggcacga cgcgcaaatt ttcgcgcctc
    24841 gcgtgccgac ctgcatgatt ttcattccca gcatcaacgg gatcagccat aacccggcgg
    24901 aacgcaccaa tattaccgac cttgccgaag gggtcaaaac gttggcactc atgctttatc
    24961 aacttgcctg gcagaaataa ggagttataa atgggatatt taaataacgt caccggttac
    25021 cgcgatgatt tactggctaa ccgtgcgatt gtgaaacacg gtaatttcgc actgttaaca
    25081 ccagacggtc tggtaaaaaa tattattccg ggctttgaaa attgtgacgc gacaatcctc
    25141 tccacgccaa agctgggtgc ctcttttgtt gattatctgg tcacactgca tcaaaacggc
    25201 ggcaaccaac agggcttcgg tggcgaaggc attgaaacgt tcctgtatgt gatctctgga
    25261 aatatcactg ccaaagccga agggaaaaca tttgccttaa gcgaaggtgg ctatctttat
    25321 tgcccgccag gctccttaat gacgtttgtt aacgcccagg ccgaagacag ccaaatcttt
    25381 ttatataagc gtcgctatat tccggtagaa ggtcatgcgc cgtggctggt ttctggcaat
    25441 gccagcgaac tggaacacat tcattatgaa ggcatggacg atgttattct gctggatttt
    25501 ctgcccaaag agttaggttt tgatatgaac atgcatatcc tctcttttgc gccaggtgcc
    25561 agccacggtt atatcgaaac tcacgtccag gaacacggtg cctatattct ttccggtcag
    25621 ggggtttata acctcgacaa taactggatc ccggtgaaaa aaggcgatta catctttatg
    25681 ggcgcttact ccttacaggc cggttatggc gtagggcgtg gtgaagcgtt tagttatatt
    25741 tactcaaaag attgcaaccg cgacgtagag atttaagtca taccactcgc ctgattattt
    25801 ttagctatgt tggattttgc cacggtttat tccgtggctt tttttgtagt caattcaggt
    25861 aaaaaaagat gcgatataaa tcgcatccgc tttaaaggta agagtcagtt tttaattccc
    25921 tgacctattt taatggcgca ggcaatattt cgcgcgctgt taaagagatt ggtttcaccg
    25981 ctggcgagca cttcggctag aggtgccaga cgtggcaaaa tgctgaatac cgcgtcaatg
    26041 ccgtactggt gcaccacttc cacgccatca cccaatacgc cagcaatccc aatcaccggg
    26101 acattaaact gcttcgccac cgacgccacg cccagcggcg ctttaccacc tgccgtttgc
    26161 gagtcgatgc gcccttcccc ggtgatcacc agtgctgcgc cctgcactgc ctgcgcaaga
    26221 ttgaccgcat tcaacacaat ctcaataccc ggtttaatat ccgcattgag gaataccgcc
    26281 gccgcaatgc ccataccgcc cgccgcgcca ccacccgcca tctggcagac gttgatttca
    26341 gtttgctgtt gtaaaacacg ggcgtaattt tgcagcccct gctccagctc ttcgaccatt
    26401 tccggcgttg cccctttttg cgggccgaac accgccgccg cgccgcgtgc ccctaccagc
    26461 ggattatcaa catcacatgc gacttcaata tggcagttcg ccagacgcgg atcgcattca
    26521 tccatctcaa tacttgccac gcgtgctaaa ttgccgccgt tagccgccag cacctgaccg
    26581 taggcatcaa ggaaacgcac accgagcgcc tgcgccatgc ccataccgcc gtcaaccgtc
    26641 gcgctaccac caatgccgag aataatatgg cgaatgccgt tatccagcgc atggcgaatt
    26701 aactcccccg taccaaaact ggaggccagc aacggattac gcttttcagg cgcgaccagc
    26761 atcaggccac ttgctgctgc catctcaatc accgccgttt tcccgtcgcc ggtaatgcca
    26821 taaaaagcat tcactttttc gcccatcggc ccgcagactt caagcttcac gatgttaccg
    26881 tccgtcgcgg cgaccatcgc ctccaccgtc ccttcgccgc catccgctat cggcaaacag
    26941 atatagtgcg catcgggaaa gagggtcgaa aacccggctt taattgcctg acaacatttt
    27001 tctgcactta agctctcttt aaaagagtct ggcgcaatga caatcttcat tatcaatcct
    27061 ttttcaacaa attattcgat gcgttcccac ggaataagat tatccataag aacagccagt
    27121 aaaattccga ccagtaagcc gttactgagc agaggacgaa gcgttaatgg aaggtcttgc
    27181 agatacacag gcggtaatgc cattaaaaat atgccgacaa ataacggcaa tgcgagtcga
    27241 taaatattgc gagcggtaaa cgtgatttgc tggctaaaca ccagcgcgga aaagagtaaa
    27301 ggcagataag aaaccagcat gaccgcgcta ctcactggta aagggatact gcaaaacagt
    27361 cgcgtgagtg ccggaaccag tgccaccagc aggcaaataa cgctgccata aataaacgaa
    27421 cgccgcgtgt aatcgctagt ctgggttaat aaaccaatgg atgaaacaaa cggtgaaaat
    27481 ggaattactg ccagtggcac ggttatcagt gtcataaatc cggtcgccac aaagctgcga
    27541 cgataacgcg tattccccgc gccctgctgc ggataaaaaa catccgtgcc ccgaatcgca
    27601 ccgtaggtat tgctgatatt taccagacct gtaatcaccg ccgtcagaat aatccccggc
    27661 gacaaagcac caccactgcc gagcgggaac cactgccagt gcaactcacc ggagagcgaa
    27721 tgcgaagaag gaaagcaaaa gtaccacaac aaccagccgg ttatcgtacc gaccagcagg
    27781 ccgtaacggg caaaacgttg tggcaggaaa ataatcatcg ccagtaccag gcacatcacc
    27841 gcgaccgaga gcgcgaacgg cggtaattga attttaaaat tcgggtcggc tatgccaaac
    27901 ggcagcccga gcataccttt gaaaaagata gtggtcagtt gcgcacccag catcaacata
    27961 aacaagacca tcaccgacgg cgtaaataac cgtgccaggc gatgtcctaa tccgctaaaa
    28021 ccaatcaaca tcgtcagcac gccggagagc gcaattccca ccgccagact ggtagcgata
    28081 tcgttgatcg gtgtcccgcg cgatgcttcg ccaagggtga tagtcaggat ggttccccac
    28141 cacaggccgc ccggcccctc cataatagcg cgacgatgac cgcagaacgc ctgagcgaag
    28201 caggccagtg cggtagcaag aaaagcatat tgcgtgagcg taagcaggct actttgcggc
    28261 aactgaaaag cagaaagtag cgtaggagga accacaaccg tgttgcaaaa aataaagaaa
    28321 aaccactgaa atcctgataa caggctttcg cggctgactg caaaattgaa cataaaaaca
    28381 ccttaaccgg agaatgcccg ccgcaaggac gggcattgca ggggccagat tactgctgat
    28441 gtttaaggat aaattgacct ttcggcgcaa cagggaagcc ctgttcgatg tcgtaaatca
    28501 catcaccacg taagatggtt ttcgtgatac gcgcgccaat ggtacggcca acatacgggc
    28561 tgactttgtg gcgatattcc agatcgtcat tggtaagaac atagctgcta ttcggctgaa
    28621 tgaagacgaa gtcggcatct tttcctgggg cgatacggcc tttttgctgc agaccgaaaa
    28681 tatctgctgc gttagtcgcc attaatttgc cgaacatcgg cagagacata ccgcgtttct
    28741 gtaccgcttc atcgaacatc acgtccatgc agctttgcag accggcgata ccaccccatg
    28801 ctttcatgat attgccggct ttcatttccg gcggacatgg agagtggtcg gaaaccaggc
    28861 agtcgatttc accgttaaac agtttttccc acatgccttt ctggttttcc agatcgcgga
    28921 tcggcggtga acacttcgcc agagtaccga tttcttcgaa ctgatcggta tcaagtacaa
    28981 agtaatgcgg gcaggattca caagtaacgt cctgaccttc ctgacgtgca cgagtcactt
    29041 cttcaacgcc ttccgggctg ctgatatggc aaacgtgcag acggcaaccg gcaactttcg
    29101 ccagatacag cacgcggcga atcgcttcca cttcggtaaa taccggacgc gaagccacat
    29161 agtcatgagc agttacgcga ccttcacgct tcgcttcttc gcccagttcg tcacagatca
    29221 gcgcgttttc gcagtgcacc agcaccggct gccccagttc gcccagcttc tgcgcacctt
    29281 tgaagaactg ccagtcgttt acatcacgga agtcgttgtc gataccgcga tcgccacagg
    29341 tcgcaacgaa gcatttgaag ccgacaacgc ccacttcatc cagctcatgc agacggtcga
    29401 tgttgtaaga caccaggcca ccgagttgtg ccgcatcgat agtcagcttg cctttagcgg
    29461 catcgaactt cagttcaatt gaagcgcggt caaccgttgc tggcagctgg ttgagcggca
    29521 tttcgatcat ggtggtgata ccaccttttg ccgctgcgcg agtaccagtt tcataacctt
    29581 cccagtggct acgacccggc tcagaaatat gggtgtgcgc atcaaccatg cctggagaaa
    29641 ccaccagacc agacgcatcc atcacttctt ttgcatcgcc cagatcctga ccgatagcag
    29701 caatttttcc gcctttaacg gcgatatcta caacgcgagc ttcgttttct aaaataacgg
    29761 tgccgttttt aatgattaaa tcaaaagaca taacaaactc cttgtaaaat acagattggt
    29821 tttttaactc aatgttcaat atcgggatta attaacctgt gactttttgt tctcctgttt
    29881 tttctgctgc tgtacgtttt tttaataacg cgtaggcagc gaatgcgaca ataacgccga
    29941 caaaccatga aacgcgtgat aaaggctcca tgaacggaat aaatttacca ccgagagata
    30001 aaataacggc gaccaaagtt actgagaacg cggtaaggtt aaagccgtta tcgtaatatt
    30061 tgaaatcacc tgctgcggta tacagttcat caagattaat ttgtccgcgc atcaccataa
    30121 aataatgcgc catcatgaca ccaattaccg gacccagcat tccgccgata atatcgagga
    30181 acagataaat actgtcctga ttttccatta atttccacgg gcagatcacc aggctgataa
    30241 tactggcaat cagtacgccg tttttatagg tcagtttcgt cggtgcaata gcggcaatct
    30301 gataaccggc cggaataata ttaccggtgg cgttagtgga aatggtcgtc atcagaataa
    30361 ccagtaccgc aaagaacgag gcgaacaggc tgtcccaacg ctgaacaata tccagcacgt
    30421 tccaggtgtc agcgccgtaa tgaatactgg ctccggcaat aatacatacc ccggcgaccg
    30481 caaacagaat ataggccaca actaaaccca gcgtttgccc cagcgcctgc tcacgaaacg
    30541 agtgggcgtt ttgcgtaaag tcggatgcgc tcaccgccgg tgccgcccag accgcaacca
    30601 ccgcgtttat caccaccaga aacaggaagc cactgttttc tgctttctga ataccgcccg
    30661 gaatgtagtc aaagattgga ccgatcccga ccagcgaaat cgcccaaatc gccataccgc
    30721 cgaaaacgat atagatgcac gggttaagaa tggcagtgaa tttatttaaa acttttccgc
    30781 cgccaaaacc aataccaacg ttaaccagcc agaagagtaa aaaagtaatt aatcccggta
    30841 gagaaaggcc taacagcgtg aaatcgccac cgagagtcaa aaatcccggc cagattttgc
    30901 caatcagaat caagcaggcc agtgaccccg cgtaacattg caggccaaac cacataatgg
    30961 cagcaatacc gcctcttaat aatccgggaa acagtgcacc acgtacgccg taagaagcac
    31021 gcaggatcat ggcaaacggt acgccgtatt tactgcccgc agcaccgttt aataccatta
    31081 ccgcggcaat gaaaaaggca ctgaggataa ttgccagcat aatactaaag gtagacaagc
    31141 cgagaataaa aaagccgcca accatcacat aattgggaac gttatgaacc gaacccatcc
    31201 ataaggtaaa atagttaaat gttttccagg tccgctgact ttgcgttttc ggcagtagat
    31261 cttcgctata gccgcgttgc tggaatagtt ttctctgatg ttccataaat ccctcttaat
    31321 ttcacgtatc gaataacgtt gttttaatct agctatgagg aacaaataaa ataaaaatat
    31381 tatttttaat actgaagcaa catctttggc tgctttcttt ttttaatatt gctggccgat
    31441 tcattttctg atatcggcca tttttattgc gggtatcagg ccagtttatg gttagccatt
    31501 aattccagcg cctgcactaa tgcagagtga tccaactggc tgccaccgtt tgccgcacag
    31561 gtattaaaca attcctggca ggtcgcggtg tttggcaggt tcagcgccag tgctttcgca
    31621 ctttgcagtg ccaggttgag atctttctgg tgcagcgcaa ttttgaaacc tggattaaag
    31681 gtgcgtttaa tcatgcgctc accgtgaact tccagaatac gtgaggaagc aaagccgccc
    31741 atcagcgcct ggcgcacacg taccgggtcc gcaccggctt ttgaagcaaa caacaaggct
    31801 tcagaaaccg cttcaatatt gagcgccacg ataatctgat ttgccacttt gcaggtttga
    31861 ccgtcgccgt taccgcccac gagagtgata tttttaccga gcagttcaaa cagcggtttt
    31921 acacgttcaa atactgcttc atcaccgcca accataatcg ataacgtccc ttcacgcgcg
    31981 ccgatttcac cgccggagac tggcgcatcg agataatcgc cgcccagttc attcacctga
    32041 cgagcgaaac gtttagtttc aatcggggaa atggagctca tatcaacaat ggttttgccc
    32101 ttcagcgagg ctttggtaca accattttca ccaaacagaa cgtcttcaac ctgtggcgta
    32161 tccggcacca taataaaaat gatgtccgat gcatctgtta cctggcgcgc agtttcaaca
    32221 ctaacggcgc ccagtgacag taattcatca gcaaccggac caatggttgt gacatgcagt
    32281 tgatgaccgg cacgcgccag attaatggcc atcggtgtac ccataatgcc taagccaata
    32341 aatcccagtt tcataattaa cctcttttaa atttcgcttt tcctgaattc agacaacacg
    32401 atcccctgcc aacttagcgg cctaaaaaag cattgtctga atagcgttac gttttaacgg
    32461 tacggatcca tccagcgtaa accggcttcc gtggtggttt ggggtttata ttcacagcca
    32521 acccaaccgt tgtagtcaga attttcaatt accgtaaaga gatattcgta attaatttct
    32581 ccggttcccg gttcgccgcg atgcggatta tcagcaattt gcaggtgacc aattttatcg
    32641 gcccactgag tcatggtatt agttaattca ccttccatcc gctgcatgtg ataaatgtca
    32701 tactgaattt tcaaattgca gcaaccaaca tcgtcaatca gtttcagtgc ctgccgggtt
    32761 ccggtgaaat ggaaaccagg aatatcaaaa tggttaatag gttcaatcag taataaaata
    32821 tcttctttca tcagcatatt cgcagcataa cgcaggtttt ctacaagcgt tgtgtgaatc
    32881 tgttcactgc tgaaaccagt cggcgtttta ccgaccagac agttaatttt tttattaccc
    32941 agagcacgag cataacgaat cgctgctgct acgccatccc gaaactcttc ttcacggcca
    33001 ggaatacagg caataccccg ctcgcccgcc gcccagtcac ctgccggtaa attgtgcagc
    33061 gtatgttcga gtttattact cgccagcacc tgttttaatt cttcaatgtc gtagtcataa
    33121 gggaacataa attcaacgcc gcgaaaacca cactgcgcag ctttctcaaa acgggcgaga
    33181 aaatcatatt caccaaataa catcgataaa ttagcagaga aacgtaacat tatttatctc
    33241 ccttattcat agtgcatgaa acaggcttca gtcggtgcat ccgctgcgtt atcggcgata
    33301 tcttcaaatt ccatgacgtt atccagttca ctgcccatcg aaatattggt cacacgctcg
    33361 agaataactt ccacgactac cggtacccga tattgcgcca ttaagatttt cgcctgttca
    33421 aaggctggtg caatatcttc cggtttgaaa acgcgaattg ctttacaacc taaaccttcc
    33481 gctactttta cgtggtcgac gccataaccg ttcacttcgc tggagttaat attttcgaac
    33541 gcgagctgta cgcagtaatc catatcaaag gcacgttgcg actggcgaat cagacccaga
    33601 taggcgttat tgaccagtac atggatgtac ggaatgttga actgtgcgcc aacggctaat
    33661 tcttcaatca ggaactggaa gtcgaagtca ccggaaatcg ccaccacatt gcgctctgga
    33721 tcagcggcac aaacccccag cgccgccgga atcgtccagc ccagcgggcc tgcctgccca
    33781 cagttgatcc agtggcggtc tttaaagaca tgcagcattt gtgccgcagc gatttgtgac
    33841 aggccaatgg tggtgacata acaaacatcg cggccaaagg ctttgttcat ctcttcatac
    33901 acgcgctgcg gtttcaccgg cacgttgtcg aagtgggttt tgcgcagcaa agtgcgtttg
    33961 cgctgctggc agtcggcgac ccattcttta cgacacggca gacgacccgc tttttgcatc
    34021 tcctgcgcca cttcaaccag cagcgtcagc gccgctttag catcagagac aatgccgaga
    34081 tccggacaca gcacgcggcc aatttgcgtc ggctcaatat cgatatgaac gattttgcgc
    34141 ccttcggtgt atttctctac cgaaccggta tgacggttag caaaacggtt accgatacca
    34201 aacaccatgt cggacgccag cagcgttgcg ttaccgtaac gatgcgcggt ttgcagaccc
    34261 accatcccgg ccatcagttc atgatcgtcg ggaatacagc cccagcccat cagcgttggg
    34321 atcaccggaa tgctggtcag ttcagcaaac tgttgtaaca gtgcagctgc gtcagcattg
    34381 attaccccgc ccccggcaac aatcaccgga cgttcggcct ggattaacat ttctaccgct
    34441 ttttcgatct gcatacggct ggcagcaggt ttgtagaccg gcagcggttc gtacatgtca
    34501 ggatcaaact cgatttccgc aacctgaacg tcgaacggta aatccaccag taccggaccc
    34561 ggacgaccag aacgcatcag gtgaaatgcc tgttgcagca cgcgaggcac cagcgccgct
    34621 tcacgtactg taaccgacat tttgctgacc ggtttagcaa ttgcttcaat atcaacggcc
    34681 tggaaatctt ctttatgcag acgggcgcgc ggagcctggc cggtaataca cagaatagga
    34741 atggaatcag cagaagcaga atagagcgcg gtgatcatgt cagtgcctgc tggtcccgag
    34801 gttcccagac atacgccgat attccctgcc gttgcgcggg tataaccttc cgccatgtgc
    34861 gaagcacctt ccacatgacg cgccagaatg tgacgaatac cgccgtgctt acgcatcgct
    34921 gaatagaaag gattgattgc tgctcccgga acgccgaagg cggtggtgat accttctttc
    34981 tccagcacat acattgccgc gtcaacggct ctcatttttg ccattttatt cctacctcta
    35041 ttaattggaa aaattttcca actttcaaat tttgtcaaaa aaacccgctc cgcagagcgg
    35101 gatgcgacag acactatgga tgtgctttca gtcccaacgc cgtgctgata tcgcgggcgg
    35161 tgtctctgac cagctcaccc tgactgacaa aacgatcttc tgtcagtctt gatgaaggcc
    35221 cggagatgga gatagcggca acaacactac ctacatcatc gtaaattgct gaagctatgc
    35281 aattcaggcc gacaacatgc tcttctttat ctacggtata gcccagttca cgcgcttgtt
    35341 ccaggtcctt cagcaaggtg ggcatatcca caagcgtagt tggcgtaaac tgctgcaaac
    35401 cggtttgcag aatgatgctc atcaactcct cttccgccag cggataaagc agcgccttgc
    35461 ccgcaccgga agcatgcagc ggcagacgac tgcccagcgg cgcacacatc ctgaccatcg
    35521 atttacactc taactgacca attaataccg cttcattgcc gttacggatc gcgacattga
    35581 ccgtttcgcc ggaaagtaac atcaggcggc gcataaacgg cccggcgacg gagagtacat
    35641 cgcggttatg gatgtacgct gcaccgacgt taaagacacc taatcctata tgccaccagc
    35701 ctaactgact gtcctgatag acaaaatccg ctgcctgtaa aaccttcagc aagcgaaagg
    35761 tcgtggagag cggcaaatcc agattgagag aaatatcgct aaccgacgaa cttcccccac
    35821 ttttttccag atattgcaga atcgcaattc cccgctctaa cgcctgtgcg cccttctgtg
    35881 ccacaggctc cgcctgtcct ggcctgccgc gccgtctaac ttccgtcata cctacgttaa
    35941 ctccgaccta caagtgagca taaacaatga acaattcgtt tatcttatgc aagtcggttg
    36001 caggcgtcac gcaaaacaga gttcctgttc aggaatactt tcaacatcac agttgtcact
    36061 gccgccgcga tcgatggtca gaaaatcggt gacgcgctgc caggcgaaaa gtgggtgatg
    36121 ccagacgtta cgatggtaat tcaccccctg ttcaccgttg gtaataaacg cccgcagcgt
    36181 tgacaggtct ggtttgtcgt cacctaacgc cacgactacc acaaacactt cacctttcat
    36241 cgggataaac gcctgtgtac ccaacggatg acgttcgagt tcgtgaatag tcagcggcag
    36301 attcgccggt tgcgcgcggt taatgctgat aagcgtacgg tcttgctcaa gaatctcaac
    36361 cagcgccaaa tcgtggtaac gctctaccag gccattgtta atatggaaaa aatcccgttg
    36421 ctgcgtttcg attacgtcgc cataagcact aaaggcttcc tgacttaacg gtaatacctg
    36481 aagtttcatc tttttgtcct tgtaacataa ttccgttcaa ccaccgccga ccggtcagct
    36541 tggtttcaat aacaggcaat ctaaaacctt aagagaacaa catcaataca aaagttggaa
    36601 tatttttcca aaactagatt tagtccacaa attgaacagg gttatcatgt aaaaatactg
    36661 ataaatcaga ggataagaaa atcacgcaac ttgcggattt aaaaggggat acctgaagat
    36721 gttcgatcca gaaaccttgc ggactttcat tgcggttgct gaaacaggaa gtttttcaaa
    36781 agcggcagaa cgattatgta aaaccacggc gacgatcagt tatcgcatta aacttctgga
    36841 agagaatacc ggagtagcgc tgtttttccg tacgactcgt agcgtgacgt tgacggcggc
    36901 tggcgagcat ctactttgtc aggccagaga ctggctgagc tggctggaaa gtatgcccag
    36961 cgagctgcaa caggtgaatg atggcgtgga acgccaggtg aatattgtca tcaacaacct
    37021 gctctataac ccccaggccg tcgcccagtt gctggcgtgg ctgaatgaac gttacccctt
    37081 tacccagttt cacatctccc gacaaatcta tatgggcgtc tgggattcgc tattatacga
    37141 aggtttttca ctggctatcg gcgtcacggg aaccgaggcg ctggcaaata cctttagtct
    37201 tgatccctta ggatcggtgc aatggcgctt tgtcatggcg gcggatcatc cgctggcgaa
    37261 cgttgaagag ccgctaacag aagcacagtt gcggcgcttt ccggcggtta atattgaaga
    37321 cagcgcccgc acactaacca aacgcgtcgc ctggcgattg ccggggcaaa aagagattat
    37381 tgttcccgat atggaaacga aaatcgccgc ccatctggcg ggcgttggca ttggtttttt
    37441 gccaaaatca ctttgccagt caatgatcga taatcaacaa ctggtcagcc gggtaatccc
    37501 aacgatgcgc cctccttcac cattgagtct ggcatggcgc aaatttggca gcggcaaagc
    37561 gatagaagat attgtgacct tgtttaccca gcgcaggccg gaaatcagcg gatttttaga
    37621 aattttcggc aacccacgca gttaaaaatc aagtttcctt gcgcgttggc gcgtaaaata
    37681 gcgcgcaagc tgatttgagg ttcacttttt actatgcaag agagacacac ggaacaggac
    37741 tatcgtgccc tgctgattgc tgatacgccc attattgatg ttcgcgcccc catcgagttt
    37801 gagcaaggcg caatgcctgc cgctatcaac ctgccgttaa tgaataacga tgaacgcgcc
    37861 gccgttggca cctgctataa acagcaaggg tcagacgcgg cgctggcgct gggtcataaa
    37921 ctggtggcgg gtgaaattcg tcagcagcgt atggacgcct ggcgggcagc gtgcctgcaa
    37981 aatccgcaag gtattctctg ctgcgcccgt ggcggtcagc gctcacatat tgtgcaaagc
    38041 tggttgcatg cagcggggat tgattatccg ctggtgaaag gcggttataa ggcactgcgc
    38101 cagaccgcga ttcaggcgac tattgaactg gcacaaaaac cgatagtgct gattggcggt
    38161 tgtaccggca gcggtaaaac gctgttagtg cagcaacagc cgaacggtgt tgatctggaa
    38221 gggttggcgc gtcatcgcgg ttcggcgttt ggtcgcacct tacaaccaca acttagccag
    38281 gcgagttttg aaaacctgct ggctgccgaa atgctaaaaa ccgacgcccg tcagaatttg
    38341 cgcctgtggg tgctggaaga tgaaagccgg atgatcggtt cgaatcacct gccggaatgc
    38401 ctgcgcgagc gaatgaccca ggcggcgatt gcggtagtag aagatccgtt tgagatccgt
    38461 cttgagcggc tgaacgaaga atatttcctg cgtatgcatc atgattttac ccacgcgtac
    38521 ggcgacgaac agggctggca ggagtattgc gaatacctgc atcacggact ttcggcgatt
    38581 aagcgtcggc tggggctaca gcgctataac gaactggctg caaggctgga tgcggcgctg
    38641 acaacgcaac ttaccaccgg cagcaccgac ggtcatctgg cctggctggt gccgttactt
    38701 gaagaatatt acgacccgat gtatcgctat cagcttgaga aaaaagcgga aaaagttgtc
    38761 tttcgcggtg agtgggcgga agtggcggaa tgggttaaga cgcgataatg agggacgtat
    38821 ttgtattagt cgcgacttga tctgacacat ggccttgaaa ggttgagagt taccggtttt
    38881 gatatgagtg tcgaatcctt atccaaaaca agaggtaact ctcatgcttc atactgccaa
    38941 tcccgtcatc aaacacaaag ccggtttgct caatctggct gaagaactca gcaacgtatc
    39001 aaaagcctgt aaaatcatgg gcgtttcgcg tgatacgttt tatcgttacc gcgaactggc
    39061 cgctgaaggc ggtgtggatg cgcagattaa tcgtagtcgc cgcgctccta accttaagaa
    39121 ccgtaccgat gaggcaattg aacaggctgt tgttgattac gccgtcgctt tcccgacaca
    39181 tggtcagcac cgggccagca acgagctgcg taaacagggg gtttttatct ccgacagtgg
    39241 tgtccgttct gtctggttac tccataacct tgagaatctc aaaagacgat actgaacaaa
    39301 ttttatcagg tgacgttccg taaaaagttg tatggagttc tcgatgcagt cggcgcgact
    39361 atcagtaccc ccgctgaacc ctggactatc gggccgccgt actggggcat gtcaccctgg
    39421 cgtgccgctg gttttccgct aatggtttat ttcctctttc tttcccggcg agaatttatt
    39481 ttaatccgga aggattattt attttcagat agggactcac attaaacacg taacatttta
    39541 attaatgcat ggctgaatat gtattacaca atgtcagata tcgtaaaaga ccagtgcaat
    39601 tctatgtaaa ctctctggct gttcatttta tttgttgttt cagggtcgga actcaccctc
    39661 aataaacaac tgaatacgag tagatattca tcgtctgagc tatatggctt tacacaatag
    39721 ccgacatgtc ttttctcagt cagggattat acttttcctc actacgaagt tgacgacttc
    39781 gatatgggat agactcttaa ttcaagcaat tactctgcac aagcatcagg taacccagct
    39841 ccaatatttg ttataaaata atgcgtaaca ttaatttcac caacaaaaaa aaggtttacg
    39901 ctatcccctt gccctggtat tttaccatat ttacaccaca ctttttttgt atctgtatca
    39961 ttctctgata cgattttcgc cacttgttcc ctggccctca cagtcaatgt tttcttaaac
    40021 ctccccccat catcgattgt tagggaaaaa taagaaaaaa aataatcatc ttctacattt
    40081 gtggatattg atatatccac cgctacgtgc ggaaaaaaat ctgggagcca tgtaaaaacc
    40141 ccatcacttt ctggtattgc catatagttt atgtaatgct tacttacaag agattcatct
    40201 ttgtgtaaat aaagataag
//
LOCUS       DAZGHF010000027        38468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGHF010000027 DAZGHF010000000
VERSION     DAZGHF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.027"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            171..1826
                     /gene="aslA"
                     /locus_tag="ACHK3A_001562"
     CDS             171..1826
                     /gene="aslA"
                     /locus_tag="ACHK3A_001562"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO8739662.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1984..3219)
                     /gene="aslB"
                     /locus_tag="ACHK3A_001563"
     CDS             complement(1984..3219)
                     /gene="aslB"
                     /locus_tag="ACHK3A_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO8739663.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKVFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(3366..3442)
                     /locus_tag="ACHK3A_001564"
     tRNA            complement(3366..3442)
                     /locus_tag="ACHK3A_001564"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3406..3408),aa:Pro,seq:tgg)
     gene            complement(3485..3571)
                     /locus_tag="ACHK3A_001565"
     tRNA            complement(3485..3571)
                     /locus_tag="ACHK3A_001565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3535..3537),aa:Leu,seq:cag)
     gene            complement(3592..3667)
                     /locus_tag="ACHK3A_001566"
     tRNA            complement(3592..3667)
                     /locus_tag="ACHK3A_001566"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3632..3634),aa:His,seq:gtg)
     gene            complement(3726..3802)
                     /locus_tag="ACHK3A_001567"
     tRNA            complement(3726..3802)
                     /locus_tag="ACHK3A_001567"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3766..3768),aa:Arg,seq:ccg)
     gene            complement(3905..5290)
                     /gene="yifK"
                     /locus_tag="ACHK3A_001568"
     CDS             complement(3905..5290)
                     /gene="yifK"
                     /locus_tag="ACHK3A_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8739664.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEK"
     gene            complement(5481..6221)
                     /gene="wecG"
                     /locus_tag="ACHK3A_001569"
     CDS             complement(5481..6221)
                     /gene="wecG"
                     /locus_tag="ACHK3A_001569"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO8739665.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6224..7576)
                     /gene="wzyE"
                     /locus_tag="ACHK3A_001570"
     CDS             complement(6224..7576)
                     /gene="wzyE"
                     /locus_tag="ACHK3A_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO8739666.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7573..8652)
                     /gene="rffT"
                     /locus_tag="ACHK3A_001571"
                     /gene_synonym="wecF"
     CDS             complement(7573..8652)
                     /gene="rffT"
                     /locus_tag="ACHK3A_001571"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO8739667.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCLALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMVNDRQREVKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8649..9899)
                     /gene="wzxE"
                     /locus_tag="ACHK3A_001572"
     CDS             complement(8649..9899)
                     /gene="wzxE"
                     /locus_tag="ACHK3A_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO8739668.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQELVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9901..11031)
                     /gene="rffA"
                     /locus_tag="ACHK3A_001573"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9901..11031)
                     /gene="rffA"
                     /locus_tag="ACHK3A_001573"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO8739669.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKYNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11036..11710)
                     /gene="rffC"
                     /locus_tag="ACHK3A_001574"
                     /gene_synonym="wecD"
     CDS             complement(11036..11710)
                     /gene="rffC"
                     /locus_tag="ACHK3A_001574"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO8739670.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPEAPLLTPDALAPWSRV
                     QAKIAASNTDELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRSASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11688..12569)
                     /gene="rfbA"
                     /locus_tag="ACHK3A_001575"
     CDS             complement(11688..12569)
                     /gene="rfbA"
                     /locus_tag="ACHK3A_001575"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO8739671.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12588..13655)
                     /gene="rffG"
                     /locus_tag="ACHK3A_001576"
     CDS             complement(12588..13655)
                     /gene="rffG"
                     /locus_tag="ACHK3A_001576"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO8739672.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFVETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SVSKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13655..14917)
                     /gene="wecC"
                     /locus_tag="ACHK3A_001577"
     CDS             complement(13655..14917)
                     /gene="wecC"
                     /locus_tag="ACHK3A_001577"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO8739673.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14914..16044)
                     /gene="wecB"
                     /locus_tag="ACHK3A_001578"
     CDS             complement(14914..16044)
                     /gene="wecB"
                     /locus_tag="ACHK3A_001578"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO8739674.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16100..17146)
                     /gene="wzzE"
                     /locus_tag="ACHK3A_001579"
     CDS             complement(16100..17146)
                     /gene="wzzE"
                     /locus_tag="ACHK3A_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO8739675.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYFRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17158..18261)
                     /gene="wecA"
                     /locus_tag="ACHK3A_001580"
     CDS             complement(17158..18261)
                     /gene="wecA"
                     /locus_tag="ACHK3A_001580"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO8739676.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18501..19760)
                     /gene="rho"
                     /locus_tag="ACHK3A_001581"
     CDS             complement(18501..19760)
                     /gene="rho"
                     /locus_tag="ACHK3A_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO8739677.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19845..19946)
                     /gene="rhoL"
                     /locus_tag="ACHK3A_001582"
     CDS             complement(19845..19946)
                     /gene="rhoL"
                     /locus_tag="ACHK3A_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO8739678.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20087..20416)
                     /gene="trxA"
                     /locus_tag="ACHK3A_001583"
     CDS             complement(20087..20416)
                     /gene="trxA"
                     /locus_tag="ACHK3A_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO8739679.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20547..21812
                     /gene="rhlB"
                     /locus_tag="ACHK3A_001584"
     CDS             20547..21812
                     /gene="rhlB"
                     /locus_tag="ACHK3A_001584"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO8739680.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAQATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21946..23430
                     /gene="gppA"
                     /locus_tag="ACHK3A_001585"
     CDS             21946..23430
                     /gene="gppA"
                     /locus_tag="ACHK3A_001585"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO8739681.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFYDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23477..25498)
                     /gene="rep"
                     /locus_tag="ACHK3A_001586"
     CDS             complement(23477..25498)
                     /gene="rep"
                     /locus_tag="ACHK3A_001586"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO8739682.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25585..25866
                     /gene="ppiC"
                     /locus_tag="ACHK3A_001587"
     CDS             25585..25866
                     /gene="ppiC"
                     /locus_tag="ACHK3A_001587"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO8739683.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25912..27387)
                     /gene="ilvC"
                     /locus_tag="ACHK3A_001588"
     CDS             complement(25912..27387)
                     /gene="ilvC"
                     /locus_tag="ACHK3A_001588"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO8739684.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETVTEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27537..28427
                     /gene="ilvY"
                     /locus_tag="ACHK3A_001589"
     CDS             27537..28427
                     /gene="ilvY"
                     /locus_tag="ACHK3A_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO8739685.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLSTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28424..29968)
                     /gene="ilvA"
                     /locus_tag="ACHK3A_001590"
     CDS             complement(28424..29968)
                     /gene="ilvA"
                     /locus_tag="ACHK3A_001590"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO8739686.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29971..31821)
                     /gene="ilvD"
                     /locus_tag="ACHK3A_001591"
     CDS             complement(29971..31821)
                     /gene="ilvD"
                     /locus_tag="ACHK3A_001591"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO8739687.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31886..32815)
                     /gene="ilvE"
                     /locus_tag="ACHK3A_001592"
     CDS             complement(31886..32815)
                     /gene="ilvE"
                     /locus_tag="ACHK3A_001592"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO8739688.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32835..33098)
                     /gene="ilvM"
                     /locus_tag="ACHK3A_001593"
     CDS             complement(32835..33098)
                     /gene="ilvM"
                     /locus_tag="ACHK3A_001593"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO8739689.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33095..34741)
                     /gene="ilvG"
                     /locus_tag="ACHK3A_001594"
     CDS             complement(33095..34741)
                     /gene="ilvG"
                     /locus_tag="ACHK3A_001594"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO8739690.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34744..34794)
                     /gene="ilvX"
                     /locus_tag="ACHK3A_001595"
     CDS             complement(34744..34794)
                     /gene="ilvX"
                     /locus_tag="ACHK3A_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO8739691.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34881..34979)
                     /gene="ilvL"
                     /locus_tag="ACHK3A_001596"
     CDS             complement(34881..34979)
                     /gene="ilvL"
                     /locus_tag="ACHK3A_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO8739692.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            35332..36852
                     /gene="yifB"
                     /locus_tag="ACHK3A_001597"
     CDS             35332..36852
                     /gene="yifB"
                     /locus_tag="ACHK3A_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO8739693.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVQGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36878..37216)
                     /gene="maoP"
                     /locus_tag="ACHK3A_001598"
     CDS             complement(36878..37216)
                     /gene="maoP"
                     /locus_tag="ACHK3A_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO8739694.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37335..38174
                     /gene="hdfR"
                     /locus_tag="ACHK3A_001599"
     CDS             37335..38174
                     /gene="hdfR"
                     /locus_tag="ACHK3A_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO8739695.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38269..38344)
                     /locus_tag="ACHK3A_001600"
     tRNA            complement(38269..38344)
                     /locus_tag="ACHK3A_001600"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38309..38311),aa:Trp,seq:cca)
     gene            complement(38353..38429)
                     /locus_tag="ACHK3A_001601"
     tRNA            complement(38353..38429)
                     /locus_tag="ACHK3A_001601"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38393..38395),aa:Asp,seq:gtc)
BASE COUNT         9423 a        10499 c         9707 g         8839 t
ORIGIN      
        1 agaggtagga ttgaaaacgc tctcctgatt ttccaattca ttttctagat aaataaataa
       61 tttattttta tcactattat ttatgcaatc atcctgtcag ggagagggat ctcaattatc
      121 aatgcttaat tacgtcatca ttttgacgac atgaaaccgg agcattttgc atggaatttt
      181 cgttttcacc caaacttctt gttgtggctg tcgccgccgc tcttccactc atggccaacg
      241 cagcagatac cccgtcaacc gccaccgcac gcaaaggctt tgccggatac gatcacccaa
      301 accagtatct ggttaaaccg gcgaccacta ttgccgacaa catgatgccg gtgatgcagc
      361 atcctgcaca agacaaagaa acccagcaga agctggcaga acttgagaaa aaaaccggta
      421 agaaaccgaa cgtggttgtt ttcttgctgg atgatgtggg ctggatggat gttggcttta
      481 acggcggcgg cgtggcggtg ggtaacccta caccagatat cgacgccgtt gccagccagg
      541 ggctgatttt aacttcggcg tattctcaac cgagttcttc cccaacccgc gccacgattc
      601 ttaccggaca atactccatc caccacggca ttctgatgcc gccaatgtac gggcaaccgg
      661 gcggtctgca agggttaacc acgctgccgc agttgctgca cgatcagggc tacgtcactc
      721 aggccatcgg gaaatggcat atgggggaaa acaaagagtc gcagccgcag aacgttggct
      781 ttgatgattt ccgcggcttt aactcggtat ccgatatgta taccgaatgg cgtgatgttc
      841 acgtcaaccc ggaagtcgcc ctgagcccgg atcgttctga atacatccaa aaactgccgt
      901 tcagcaaaga tgacgttcat gccgtgcgcg gcggtgaaca ggaagccatt gccgacatta
      961 cgccgaaata tatggaagac ctggatcaac gctggatgga atatggcgtt aagttcctcg
     1021 acaagatggc gaagagcgat aagcctttct tcctctacta cggcactcgc ggctgtcact
     1081 tcgataacta cccaaatgcg aaatatgcgg gtagctctcc ggcgcgcacc tcttacggcg
     1141 actgcatggt ggagatgaac gatatcttcg ccaatctgta taaagcactg gagaaaaacg
     1201 gtcagcttga taacacgctg attgtgttca cctctgataa cggtccggaa gccgaagttc
     1261 cgccgcacgg tcgcacaccg ttccgcggtg cgaaaggttc tacatgggaa ggcggcgttc
     1321 gtgtgccaac cttcgtttac tggaaaggca tgatccaacc gcgtaaatct gacggcattg
     1381 tcgatctggc ggatctcttc cctacggcgc tggatctggc ggggcatcct ggggcgaaag
     1441 tagcgaattt agtaccgaaa accaccttta tcgatggtgt ggaccagaca tctttcttcc
     1501 tgggaacaaa tggtcagtct aaccgtaagg ccgagcacta cttcctcaac ggtaaacttt
     1561 ctgctgtgcg tatggatgag ttcaagtatc acgtcctgat tcagcaacct tacgcttata
     1621 cccagagcgg atatcagggt ggattcaccg gcacagtaat gcaaacggcg ggatcgtcgg
     1681 tgtttaacct gtacaccgat ccgcaggaaa gcgactctat cggcgtgcgc catattccga
     1741 tgggtgtacc gctacagacc gaaatgcacg cgtatatgga gatcctgaaa aaatatccac
     1801 cacgcgcgca gattaaatct gactaagccg gcgctatcgc tgagatctgc ctttgccgga
     1861 tgcgatgctg acgcatctta tccagcctac agaacgctgc aatttattga atttgcaatg
     1921 attgtaggcc ggataaggcg ttcacgccgc atccggcaat caaccgcagg cggccgccga
     1981 tttctactta ttcaccacca tcaaatgcgc ctgcataatg tcgctggccg gacgaccgtg
     2041 cgccagcaaa tcagccattg ctttaagata tggcggtaga tggcggaaat aacgctgata
     2101 cccggcacac aaataattca gcccgggttt gccgctggca tcgagcataa agcggtgttt
     2161 cgggcagcct ccccagcacg cttttaacac gttgcaactg cgacactgtg ccggtaactg
     2221 cttgaattta tcttcaccaa atgcctgctg ttgcggggaa tcaatcattt ctgcaatcgt
     2281 ctgctgaagc atattcccta gtcgatactg cggataaaca tagtgatcgc aggcgtaaac
     2341 atcgccgttg tgttcaacaa tcaccgagcg cccacaggtt ggctgatgat ggcaaaccgc
     2401 gcccggtgcg ccgacaaaat tggcgaacgc ccattcgata ttcattacga aaaccttgcc
     2461 gacgtcgcgc ttaatccagt ggtcgaaaat cgccaccaga aactcaccga actcttgcgg
     2521 gcacaccgac cattccgtca gttcgccctg aatatcgccg ggagcatgta gcttaagtcc
     2581 agcatgagca gccgtttcat cagccaggcg ctcgaccacc ggaataaact ggatgaactc
     2641 aacacctgca tcacacagaa aatcatagac ttgcaaaggt tgtaacgcac tggtgcgatt
     2701 tacgcagacc agcacgttat agtcgacatg atgtttttgc aggagcgtca gggcacgcat
     2761 caccagctta tgcgttggtc tgccgccttt ggtcacgcga tattgattgt ggatctcagc
     2821 cggaccatcc agcgataacc caacaagaaa atgattttct gccagaaatg cacaccattc
     2881 gtcatcaagc agcacgccgt tagtctggaa gctgttactt atcttcctgc cagcaccata
     2941 tttcgcctgt agcgccacgg cacggcggta aaaatccagc ccgagtaacg ttggttcgcc
     3001 gccctgccag gtaaaagcca cttcgttttg ggtttcgctg gcagcgatat agtgacggac
     3061 atacgcttcc agcgtgtcat cgtccatatg cgtgactggc ttttcgcggt aaagggattg
     3121 tttttcgaga taaaaacagt agtcacagtt cagattacaa tcggaaccgc tcggtttcgc
     3181 catcacatga aaagcacgcg ttggaacctg ttgcagcatg gtcgctcctt gcctgaagtc
     3241 atccttcaat aagttaaggg cgaaatcata ccgtggcgca atgcgttatt acgggcgttt
     3301 attgcgaagg gaaagtgacg aaatgcacag aaaacaaaaa accccgccga agcggggttc
     3361 aaaattggtc ggcgagagag gattcgaacc tccgacccac tggtcccaaa ccagttgcgc
     3421 taccaagctg cgctactcgc cgaaatactg ctttttgaat ttttagttca attctttaaa
     3481 gtcgtggtgc gatggggggg acttgaaccc ccacgtccgt aaggacacta acacctgaag
     3541 ctagcgcgtc taccaattcc gccaccttcg cattgtcaca acttctaata atggggtggc
     3601 taatgggatt cgaacccacg acaactggaa tcacaatcca gggctctacc aactgagcta
     3661 tagccaccac tacaaatctt gttacgcggt attactacca ccgcagctca agcgccggga
     3721 ctaaatggcg cgcccgacag gattcgaacc tgagacctct gcctccggag ggcagcgctc
     3781 tatccagctg agctacgggc gcttagcgcc gttgcggggg tggataatac ggacttccca
     3841 cccctctgtc tagtcccttt ttaaataaat tgcgcgtttg gttacggttt gtgcgctttg
     3901 ctgcttattt ctccagttta tgcgctttac cgtggcgatt aagcccaaaa actttataaa
     3961 tcgccgtcac cgtcagcata aagatgatgc cgacaaacag cgacatacgc gtatcttcat
     4021 taaagtacat gccgatcaaa acgcaaatca ggaatgccat tgttacgtag ttggcccacg
     4081 ggaacaggat tgagcggaac ggatggctgg caattgccgc tttatgcgca cgacgaaaac
     4141 gcagctggct aatcaatatc acaaaccatg gcaccatccc cggaagcacg ctggcactgt
     4201 agacgtagac aaacacacgc tgcggattgg gaatgatgta gttcaggcat gagccaatta
     4261 acaggatagc aatagatact gccacacccg caaccggtac gccgtgacgg gaaactttcg
     4321 ccatcgccgt cggtaactga cggtttttcg ccaatgcgta gagcatacgt ccgcaactgt
     4381 acatgccgct gttacagcca gagagcgcag ccgtcagcac cacaaagttg ataatgcccg
     4441 ccgctgcggt aatgccgatt ttggcgaaag tcagtacgaa cgggctgccg ttactgccta
     4501 tttcattcca cgggaagatg gtgacgataa cgaaaatcgc gcctacgtag aaaatcagga
     4561 tacgccacaa cactttgcct acggcactgc gcagcgtcac ctgcggattc ttcgcttcac
     4621 cggcagtaat gccaatcagc tccacgccct ggtaggacgc caccacaata cacaatgcgg
     4681 tcaggaaccc tttccagcca cccgcaaaga aaccgccatg ctctgtgaga ttgctaaaac
     4741 caatcgactg cccgccattg ccaaaaccaa agaaaatcac gcccaggcca atgacaatca
     4801 tcacgataat cgtggtgact ttgatcatcg cgaaccagaa ctcgatttcg ccgtacaacc
     4861 gcactgccgc cagattcgcc agcgccacca gcgccaccgc gatcaatgcg ggtatccact
     4921 gcgccatctc cgggaaccag aactggacat aaacgccaat ggcggtgatt tctgagatcc
     4981 ccaccgccat ccacataaac cagtaagacc aggcggtgag atagccaaaa aacgggctca
     5041 tataacgatg cgcataaacg gcgaacgaac cggtaaccgg ttcgaggaac aacatttcgc
     5101 ccattgaacg catgatgaaa aagacgaaca gcccggcgat gatataggcc aacaatacgg
     5161 atggcccggc ccatttcagg gtactggcgg cccccataaa caggccgacg ccaatggtgc
     5221 cccccagggc gatgagttcg atatgtcgag cttccagccc acgctgtagc tctggtttgt
     5281 tatctgccat aaatcctcgt gttgtgtttg catgctttcc ggtgttaccg gttatcgtta
     5341 tgggtacatc gagtgttgcg aatgttttcg taattcagga gaaatggcaa ataaagcatt
     5401 aaaaatttga atgctttgtg taataaaaaa gcagacaggc gacggagtga ccactccgtc
     5461 gctttacaaa gagaggaaaa tcagaggttg ccggtgtagt gccagcgtaa ataacgcagc
     5521 aaacgaagct gacgcttaat gcggctcggc tgcgaaagca ggcggtagag ccactccagc
     5581 cccagcgttt gccagatttt cggtgcgcgt tttacgtgac cggtgaaaac atcgtaggtc
     5641 ccgccaacgc ccatatacag cgcatctgga tgcaccagac ggcagtcgcg catgaagatc
     5701 tcctgctttg gcgatcccat cgcaacggtg acgatttgcg ctccgctggc atgaatgcgt
     5761 tcaaacagcg cctgacgctg ctcgggttta aaataaccat cctgactgcc aacgatattc
     5821 acattccact ggttgcgcag tttagcttca gtttgcgcca gcacttcagg tttaccgccc
     5881 acaagaaata ccggcgtccc ttctttgcct gcgcgcgcca tcagctcttc ccagagatcg
     5941 gcaccggcaa cgcgggaaac ctgcgcctgc gggtactttt tacgtactga acgtacaacg
     6001 ctgatgccat ccgcatattt aaattcggca gcgttaatta gctccctgac ctcagcatta
     6061 tcttcaatgg tcagcatttt ttcggcatta atggcaacca gcgttccctg cttaagctgc
     6121 ccgtcagcaa acagataatc gagggcgtgc tgcatatcac gccaaccaat caactgtaag
     6181 ccacgcagcg tataggtcgg tgccgtggtg ttgttattca ttgttatcct tcaacctgcg
     6241 tccggagcga tgattttgta cgtttatgaa tgagtccggc gctttcaaaa agccagtaca
     6301 acagttttgc gatcatcaga catgcgccga agaccacgat aaaaaagact acgcgtgaga
     6361 caaacgaatc cagcccttca cgtgccagca cgatcatatt gaagatcgcc ccgaagcaga
     6421 aactgtgcaa tatcgcagcc ttatagcgat tagtctcgcg gttgcccagc tcatacagcc
     6481 agtcgaacca tttgatgatc agaccaacca caatcgcccc gagcgggatg aacaacgcgc
     6541 cgcccatcac caccagtgag cctataagcg taggcgagat cgccagcccg gagtggttat
     6601 tcagcacttc ccaggtaaag tagttggcgg agttcagtac catactcggg cgacccggcc
     6661 acagccagga agggataaag acatagaaat cgcggacaat gggagccagg ccctggaagt
     6721 cgatgttgtc gtagttctgc aacagcaacg ccagattctc ccacggcgaa aaggtatcgc
     6781 gggtgagata gagaaacgta tagaacgctt catcgccgct cacattcatt ccataacgtt
     6841 ttagcgccag ccagaacatg cccacaatcc ccagcacgcc cgccgccgcc agcatccaca
     6901 acgaaatcca gccgcgaata atgccaataa acaggaagat agcgaatgcg atgatgatat
     6961 tggcgcgagt gccgccgaca atcatgtaag tcaacaagcc aaaggcaacc gtgctgacga
     7021 ggaaaaacag ccacgctttg ctatcctggc gcagaaaata aaccaccagc atcgccggga
     7081 tgaaaaagta aaagaagcgt tttaatgcca cgccggagac ttcgctggaa aagatctgac
     7141 tgtaagaatt aagccggaac agcaaaaagc cgttgtgcat gaagaagatg ccgacgctca
     7201 ccagagcgat gcccatcagg atcacccacg tgagattggt ctccacgcgg ttcatggtaa
     7261 acagcggacg gcgcggtgca tcagcaacgc gtttgcgtag acgggttttg taggtgacat
     7321 agtaaaccgc gtagaagcag cctgcagaaa gcaacgcctg caacaagatt tctggcggcg
     7381 cgacaccaac atcaaagcga aataccagca cgctggtcag cggaaagccg aagaaaaagg
     7441 tgagcaaaaa cagcaatgaa aagaagacat tgaagttaaa gcgcacacgg cgaaactcaa
     7501 accaggtcag cgtggcaata aacaacgtgc agagcagcca gacaacaaac aggccactga
     7561 attgcagcag actcatgcga cctccccggc ggcaatcgcc aacgcccgct gccagccttg
     7621 tagatagtta gggctaaaga aggcaatggt gtttttatcc accgacgcca actggcgctg
     7681 cgcttcacgc acaatatcct cgttgagatc atcggtagta aacagtactg gcaaatgctg
     7741 ttccgtcata tcctgccaga acggattttc ccggttaagc acacaaggaa tgcccgcctg
     7801 aatcagtaag caaagcgtac caatgccctg ctggcgggca aaaataaagt aaccgagatc
     7861 gcactgacga agtagcgtca gataggcgtc aaattccagt ttttcgctca gaacttgcag
     7921 attttcttcg ctgaataact ccagtcccgc ctgacgaact tcctcgatgt acgcttcgtt
     7981 attaggcgga tatcccatcg gcaccaccac ttttaccgta tcgccaaatt gctgatgaac
     8041 ggcgcgcaag gcagcaatat gctcattgct gcggtcaccg gagttcccca ccagaatggt
     8101 catttttact tcacgttgcc gatcgttcac catcgtatta agagaagagt ccatccgcgt
     8161 cgggaagtac agcagttcgc cccgcacctt tgggtgcgtt ttggcaaaaa agctcaaatc
     8221 accgcgggtg gcaaatacac agccgactcg cttttgcgcc aggcgacgta gtgggtaaaa
     8281 aagcttatat ctcaagccac tggaaagttc gtacaggtct gccccccaga tatgccagta
     8341 aaactggctg ggcttaatgc caccactcag cagagccagc cacagtgtgg gattgaactg
     8401 accgtggaag aaaaaacgct gctgacggtt agcttttgct ttcgcgatga ccgcttccgc
     8461 cagcgatttt ttcccaggga aaaattgcac agaaagcgcc agacagctat cacttaagcc
     8521 gtcgtccttg ccaacaacca taaactcgcg cgcgtgctcg ctcgtcgcgg ccagcgcgtc
     8581 gttgaaaaac cgcagaacgg ttcggttatg gtgagggata tccgatccca gtacgtgaat
     8641 cagtacagtc atgcccgcct acgccagagt aaaaacacgc cacaacaaag agaaaaatag
     8701 acgatatagg ttgccatata ggcctgcgct gcgcccagtg caccatgcgc agggattagc
     8761 cagtgggcaa ataccatcaa taaagtgaac tggctgactt ccgccagaat ataaaaccgc
     8821 agtgacgctt tggcgatcac cagataacca aagacataag cgcccacttt taacacatca
     8881 cccactaact gccaggcaaa gagatcgcgc atagcggtaa atttattcga caacagcagc
     8941 cagatagcaa aatcacgcag caaccagacg gtaaaactcg ccgccgccac tgccggtaag
     9001 acgaatttta gcgatttaac cacttcccgg gtgatatcgc gcttttccgt tagccgcgac
     9061 aacgtgggca gcaaatatac gctgaacgat gccgtaataa attgcaggta ggcatcggaa
     9121 atactgctca ccccttgcca gatccccacc tcatcccagc tatactgcgc cgccagcagt
     9181 ttacgcatca tgatgtaagc aacaggcaag gtcaccgacg taatcaacgc catgagcgta
     9241 aatttgctca actgccctgc cagaccgtta tcccagctgg gtttcagata gcttaacggg
     9301 atgacaccac gtttgatcaa catgatggcg gcaggaatta ccaccagcgc gggaatcagc
     9361 gccagaccca gcaacgcccc ttcataaccg cccaaacggt aactgacgta gtacgcaaga
     9421 acgccaatca agctgccaac aatcagcgat aacgcattac ctgcggcatc gcgaaagcct
     9481 ttcatcaacg ccagtaacag gttgccccag gcgatcccca tttgcaccaa cgccactaaa
     9541 cgcaccagct cctgatagtc ggtattacca aacaatccct ggctgattgg cgcagctgcc
     9601 agcacaaaaa ccagcgccat cagcgtagag aaaccaagta ccatcgctga tgaagtgccg
     9661 accacgcggc gcagctgttg gggattatca tggtactggg caacgtattt ggttacgccg
     9721 ttaaagatgc ctgccccggc aagcacgccg agcacggtaa tcaactggcg gaaatttgcc
     9781 gccagcccaa gccccgccgg accaaatgac actgccagca acttaccgac cagtaacccg
     9841 gcaccaatct tgaccagtgt actggccgca gtccacaagg acgcttttgc caacgacata
     9901 tcaggagaag tagttcaaca aggtcgcaat taccgtacgc tgattgacgg gcgacaggtt
     9961 gtagaacaac ggcaggcgca gcaggcgctc gctctctttg gtggtgtagc gatcttcacc
    10021 gtggaactca ccaaagcgtt cccccgcagg gcaaccgtgc agcggaatgt aatggaacac
    10081 cgccatgatt tccgcttctt tcagaaagtt aatcaacgcg ctccggtcat caatatcccg
    10141 cagtttaatg tagaacatat gcgcgttctg cacgcagcca tcgggaatcg acggcagctc
    10201 gatacgcccg gctttcgcca gaggtgctaa cgcatcgtag tagttttgcc acagcgccag
    10261 acgttgctga ttgatacgat ccgctgcttc cagttgcgcc cacaggtacg cagcttgcaa
    10321 atcggacatc aaatagctgg agccgatatc gcgccaggta tatttatcga cctgaccacg
    10381 gaagaactgg ctgcggttag tgcctttttc acggatgatc tcggctcgtt cgatcaacgc
    10441 tttatcgtta atcagcgtcg caccgccttc accgcccgcc gtgtagtttt tggtttcatg
    10501 gaagctaaag cagccaatat gaccaatggt tcccagtgca cgccctttgt aagtggacat
    10561 aacgccctga gcggcatctt ccaccacaaa cagattatac tttttcgcca acgccataat
    10621 ggtgtccatt tcgcaggcca cacccgcgta atggaccggc acgataacgc gcgttttgtc
    10681 ggtgatcgcc gcttcaatca gcgtttcgtc gatgttcata gtgtccgggc gaacatccac
    10741 aaagacgatt ttcgcgccac gcagcacaaa ggcattggcg gtggagacaa aggtgtagct
    10801 cggcatgatc acttcatcgc caggctggat atcgagcagc agcgccgcca tctccagaga
    10861 agcggtgcag gacggcgtca acagcacttt ggcgctgcca aaacgttgtt ccagccactg
    10921 ctggcagcga cgggtaaaac cgccatcgcc acacagtttg ccgctaccca ttgccgactg
    10981 catatagtcg agttcggttc ccaccaccgg cggtgcgtta aatggaatca tgtgatcacc
    11041 tgtataacca gtacgcggtg ctttctacat tcgcaccact ttgtatgtat cgtttaagcg
    11101 cggcggtgtt gcccatctgg gtcgccaccc gcaaagttgt tttaccgcga gcatacgccc
    11161 agtttagcgc tgtttgcatc agctcagcac ctgcaccgcg tccagccagc aggccaattc
    11221 gcgcatctgt cgcattgagt tcccgtaaag agacatagcc gcgaatatcg ccggacgccg
    11281 aacgtaaaat gaggcattga tgatcaaagg tgccgcgcac ggcattttca atccactgtg
    11341 cataaaagcg accgctggcg tcaggcgcat accacggcgc acgaaaacgg ctttgcgcaa
    11401 atgcggcgct ggctaactga cgtaatgcgg gaatatcggt ctcttgtgcc actacagcac
    11461 cactatcact gacattgttt acgggtagcg ccaaatcaac ttcaccttct accagggaga
    11521 atcccagctg ttgcaaggca tccagttcat ccgtatttga tgccgcaatt ttggcctgca
    11581 cccgtgacca cggcgctaag gcgtctggcg tcaggagcgg tgcttcagga ctaatgcgca
    11641 caatggcgct gttaacacca aagaaggcgt tttcccaggt tagtggctca atactggcgc
    11701 ggacgggcac gaagtaactc cagcagatat tggccgtagc cagttttcgc cagcgagctg
    11761 gcggcgcgct tcacgccttc gtcatcaagc cagccgttac gccaggcgat ctcttccagg
    11821 caggcaatct taaagccctg gcgtttttcc accgtctgca caaaggtgct ggcttcaatc
    11881 aggctgtcgt gagtgccagt gtccagccag gcaaatccgc gcccgagcag ttcaacggtc
    11941 aggttgcccg cctcgaggta catctggttg atggaggtaa tctccagttc accacgctcc
    12001 gacggcttca cctgctttgc gtactccacg actttactgt cgtagaaata aagcccggtc
    12061 accgcccagt ttgacttcgg ctgttttggc ttttcttcca gcgagatagc gcggaaattg
    12121 tcgtcaaact ccacgacgcc aaagcgttcc ggatccatca cctgatagcc aaataccgtc
    12181 gcgccttgcg tgcgcgccgc aacatgacgc agcttcggac tgaagccctg tccgaagaag
    12241 atgttatcgc ccagcaccag acaagaaggt tcaccgttga ggaaggtttc accgatgata
    12301 aaggcctgcg ccagaccgtc cggactgggc tgttcggcat attccagctg gataccgaac
    12361 tcactaccat cgcccagcag gcgctggaaa taacctttat cttccggcgt agtgatgatg
    12421 agaatttcgc ggataccggc cagcatcagc accgacagcg gatagtaaat cattggctta
    12481 tcgtaaatcg gcaacagttg cttcgatacg ccgcgcgtaa tcggatgcaa tcgggtgccg
    12541 gaaccgcccg ccaggataat acctttcatg tttgcctccg ctgaaaatta gcctttcaga
    12601 cctaaacgct cgccctgata gctgccgtcc tgcacctgct tccaccagct ttcattagcc
    12661 agataccact gaaccgtttt acgcattcca ctttcaaagg tttcctgcgg cagccagcca
    12721 agttcacggg caattttcga agcatcaatg gcataacgca gatcatgccc cggacggtca
    12781 gcgacaaagg tgatcaggtc acgatagtgc gccacgccgt gcggcttgtt cggagccagt
    12841 tcttccagca gctcgcaaat ggtttccaca acatcgagat tcttacgctc gttgtgacca
    12901 ccaatattat aggtttcacc gactttcccg gtggtcgcca cgcaatacag cgcgcgggcg
    12961 tgatcttcca catacagcca gtcacggatt tgctgcccgt tgccatatac cggcagcggt
    13021 ttacccgcca gcgcgttgag gatcatcagc gggatcagtt tttccggaaa gtggtaaggg
    13081 ccgtagttat tcgagcagtt ggtgataagc gtcggcagac cataggtccg cagccaggcg
    13141 cgcaccagat ggtcgctgct ggcttttgac acggaataag ggctgctcgg cgcatacggc
    13201 gtggtttcgg tgaagaaatc atccgtcgag tgcaggtcac catatacttc gtcggtagag
    13261 atatgatgaa aacggaacgc tgatttttta tcttccgtca gcgcattcca gtaagcccgc
    13321 gccgcttcaa gcaatgtata agtcccgaca atgttggttt caacaaacgc tgccgggccg
    13381 tcaatagaac ggtcaacatg gctttcggct gccagatgca tgacacagtc tggctgatgc
    13441 tcagtgaata cgcgcgccag ttctgcccga tcgcagatat caactttctc aaaggcaaag
    13501 cgctcgcttt gcgcgaccgg tgccagcgac atcagatttc cggcgtaggt cagcttatcg
    13561 accactacca ccgcgtcgct cgtttcgttg atgatataac gcaccagcgc cgagccaata
    13621 aacccggcac cacctgttat cagaattttt ctcatcagcg ccagactcct ttggcatcga
    13681 cgacatactg ctgatggaca ttgtcgccat tgataacttt gaactgacta tgatcgacca
    13741 gcatcaccag cacgtctgcc gttgccagcg cctcgtcaag ctgcgccagt gtacaaagcc
    13801 cggtcagttt tttcggcaac tggtggatgt taggctcaac aaccagagtt tcgccgctat
    13861 gccactgggc gatcagttca gcgatttcca tcgctgggct ttcgcgcagg tcatcaatat
    13921 tcggtttaaa cgccagacca aagcaggcga ttttcagttc actggcgcgt ttatcggtag
    13981 cagcaaggca gtcggcaacc gccgctttca cctgatcgat aacccagaac ggtttgtgat
    14041 cgttcacttc gcgcgcggta cggataagcc gcgcctgctg cgggttctgt gccacgataa
    14101 accacggatc aacagcaatg cagtgaccgc ccacgccagg accaggctga agaatattaa
    14161 cgcgagggtg acgattcgcc aggcgaatca gttcccagac gttaattccc tgatcggcac
    14221 aaatcagcga cagttcgtta gcaaaagcga tattcacatc gcggaagctg ttttcggtga
    14281 gcttacacat ttccgccgtc cgcgagttag tgacgacaca ctcaccttcg aggaaaattt
    14341 tgtacagttc gctggcgcgg gccgaacaaa ccggcgtcat accaccaatc acgcgatcgt
    14401 ttttaatcag ctcgaccata acctggcccg gtaacacgcg ttccgggcag taagcaatgt
    14461 tgacgtccgc caactcgccc acctgctgtg ggaaagtgag atctggacgc atctctgcta
    14521 accattctgc catcttctcg gttgacccca ccggcgaggt ggattcaagg atcaccagcg
    14581 cgcctttttt cagcactggc gcaatggagc gagcagccga ttcaacgtag gtcatatctg
    14641 gctcatgatc gcctttaaac ggcgtgggta cagcaatcag ccaggcatcc gcttcaactg
    14701 gcgtcgtgct cgctcgtaaa aaaccgcctt ctacggcagt ttttactacg ctcgccaaat
    14761 caggttcgac gatatggatt tcgccacgat tgatggtatc aaccgcgtgt tggttgatat
    14821 cgacaccaat cacctgtttt tgccgtgagg caaacgcagc tgctgtaggc agcccgatgt
    14881 aacccagtcc gataacagaa atggtcgcaa aactcatagt gatatccgat tattttttaa
    14941 cgcttccaga atgcgagagc atgcctgacc atcaccatac gggttatggg cgcggctcat
    15001 agcttgatat tcgttttcgt cttttaaaag acgcgtcact tcctcgacaa ttcgctgctt
    15061 atccgtgcct accagacgca ccgtacccgc cgtcaccgct tccggacgct cagtggtatc
    15121 gcgcattacc agcaccggtt tccccagcga aggcgcttct tcctgaatgc cgcctgagtc
    15181 ggtcaaaatc agccaggcgt ggttcatcag ccagacaaac ggtaaatact cctggggatc
    15241 gatcagaatg acatttttca cgtgccccag aatgcgattg accggctctc tgacgttcgg
    15301 gttgagatgc actggataga caatctggat gtcctggtgc gtggtggcga tgtctgccag
    15361 cgcatggcag atttcttcaa agccacgacc gaagctctca cgcctgtgac cggtcaccag
    15421 aatcattttt ttattggggt cgataaacgg gtaatttgcc gccagttctg aacgcagcgt
    15481 gtcgctgctc atcacctggt cacgcaccca taacagtgca tcaatgactg tattaccagt
    15541 aatgaagatt cggctatccg caacgttttc acgcagcaag ttttgccggg aagtttcggt
    15601 tggagagaag tgatacatcg ccagatgccc ggtcaatgta cggttagcct cttccggcca
    15661 cggcgaatag agatcgcccg tgcgcagacc agcctcaacg tgaccaacag gaatacgctg
    15721 ataaaacgcc gccaggctgg ttgccagcgt cgtcgtagta tcgccgtgaa ccagcacgac
    15781 gtctggtttg aactcggcaa gaataggttt tagcccttcc agaatccgac aggttatctc
    15841 tgtaaggccc tgtcctggct gcattatgtt gagatcgtag tcaggtacaa tggaaaagag
    15901 tttcagcacc tgatcgagca tctcccgatg ctgcgcagtg acgcaaactt tagcctcaaa
    15961 aaaaggatct tttgccaacg catgcaccaa cggcgccatc ttgatggctt ccgggcgcgt
    16021 accaaataca gtcagtactt tcacatcgat tctcttcgaa taagcggcga gcgcctttgc
    16081 gctcaccgca gcagtgttgc tatttcgagc aacggcgggt taatgcgaca ccagccccga
    16141 tcagtccccc gacaatgccc cacataatca tcaggaaggc acgacgtggg ctatcgcgtt
    16201 ttaccggttc ttccggcgta cgaaaatagc gataggtctg aaaacgcgga tccagggttg
    16261 gaccaacatt cagggtgttt aacatggccc gattctgatc atagtcgaga tcaaaggccg
    16321 gaccgacggc ctgtaaattt tccagtcgag cctggagcat tggacgccca agcaggaaca
    16381 tttctgaatc aggtaattcc tcggcaggca catctgtcgc actgcgcgaa atattatgct
    16441 gctcagcaat tttcagcgcc tgttcaatac tgttcatccg gcgatcgtag atggctttcg
    16501 ccacctcttc ctgacgcttc acctgagctt tcatctggat ggtgcgtgcc gcccatgcgc
    16561 ctttcagctc atcattcaga tggctggctg cacgctggct ggcaaaagca acatactgac
    16621 gtaacaggtt attagcgtca ggcgcagttt cggcaatcag cttcacgctg tcattgaccg
    16681 cgcgggtgaa gtcaccggga ataaacacga tgttgttgat catttcatcc agcaacgccg
    16741 catcggcttt gctgttgccc accatccgct gtttgtaata gtcggtttgc agccagaact
    16801 ctctgcgggt atcccacgag gccagttgca taacaaactc tttataagct tcgtccatga
    16861 ccgatggttg gtcggcagaa gccatgtttg aacggacatc caggttacgc aaaaattgct
    16921 gctgtgagta atatcccccc agcatattca ccgttggacg atcggtaatc gctgtcgagc
    16981 tccactcctg gcgagcaaaa aaagtatacg ccagcgcgat taacgcaaac gccagcccca
    17041 tgccaataat ccacagcttc ccagcccaca aggtacgaaa caacccacga atatccagtt
    17101 cattttcagc gtcttcggcc ggtttcccag gcattggttg tgtcatcaca tcctcattta
    17161 tttggttaaa ttggggctgc caccacgatt tctacgcagt ctgcgtttta cgcgcttaat
    17221 aaagcgagca actttccagg cacgcttgat gcaatatcca tagaggaaga atgctagcaa
    17281 aaagagcacc agcatgaccc actccgggac aaaatgagaa tattctgcca gcacgccaat
    17341 ggaagcgagc agtgctgcgg caagggtaat cagcacaaac gcctggcggg aagtaaaccc
    17401 ggcacgcatg atcaaatggt gaatatgctg acggtctgga gagaatgggc tcatgccttt
    17461 acgcaggcga cggtacataa tcgccaccat atccattagc ggaatggcga ttatccacaa
    17521 agcggtaacc gggctgatgg gatgagtttt gccctgggtc gtttcgagca ggatccagat
    17581 aacggtaaaa ccaatcagcg tactgcccgc atcccccata aagactttgt agcggcgacc
    17641 caggatacca aggttaagca tgatgtatgg caggatggcg gcgatcatcg caaagcacca
    17701 gattgcgaga ctggtttgcc cgtcgaacca caaaatcata ccgattgctg caaacgagac
    17761 gcaggacaac ccacccagca agccatcaat gccatcaacc atgttgaacg cattaatggc
    17821 cgcccagacg gcaaatagcg tcaggaagta accaaacggt ccgagcacca tctcccagga
    17881 gccaaagata taacctaggc tactgagata aagtttgccg aacaccatca taacaatgcc
    17941 aacagcggcc tgtatggtgg cacggatttt tacgctgata tcaaaacggt catccagcgc
    18001 gccaatgaaa acaagcacac cagcacaagc gagatagaga gatgcatgcg gaatatagta
    18061 atcgacaatt ccgaacgtga agcaaatccc tgcgtaaacc gaaatccccc caacgagagg
    18121 tatcaatccc tggtgacgtt tgcggaagtt aggtttatcc actaaaccga ctttttttgc
    18181 caccttacgg gcaaaaaaca gaaacagtgt cgtgaataaa aaaatactga tgagatcagt
    18241 actcactgtc agtaaattca caatgcatgc tctcagagaa aattattagc agaagtataa
    18301 ccacgaagac ctttattcag aagggaaagt ccgttccgaa cccaccagtc ctgtgcaaaa
    18361 attaaccatt tgatatgcaa attgcataac acaccagcaa aatccaggaa ttttcctaac
    18421 attgccagcg cggcattaag attacagata taaaagcaaa acgccacgta aacacgtggc
    18481 gtttttggca taagacaaat ttatgagcgt ttcatcattt cgaagaaatc gtcattggtc
    18541 ttggtcattg ccagtttatt aatgaggaat tccattgcat cgatttcgcc catcgggtga
    18601 atgattttgc gcaggatcca cattttctgc agttcttcct gagtagtgag cagctcttct
    18661 ttacgggtac cggaacggtt gtagtcgatt gccgggaaga cgcgtttttc agcgatctta
    18721 cgagagaggt gcagttccat gttgcctgta cctttaaact cttcgtagat aacttcgtcc
    18781 atcttggaac cggtatcgat aagcgcggta gcgatgatgg tcaggctgcc gccctcttcc
    18841 acgttacgcg ccgcaccgaa gaagcgtttc ggacgatgca gggcgttggc atccacacca
    18901 ccggtcagca ctttacctga cgccggaaca acggtgttgt aagcgcgcgc cagacgagtg
    18961 atggagtcga gcaggatgat aacgtctttc ttgtgctcaa caaggcgttt cgccttctcg
    19021 atcaccattt ccgcgacctg aacgtggcga gatgctggtt cgtcaaaggt agaagcaaca
    19081 acttcacctt ttaccagacg ctgcatctcg gttacttctt ccggacgttc gtcgatcagc
    19141 agaaccatca gcacacaatc cgggtggttg taagcaatgc tctgagcaat gttctgcagc
    19201 agcatggttt taccggcttt cggcggtgcc acaatcagac cacgctgacc acgaccgata
    19261 ggtgatgcca gatccagtac gcgagcggtt aaatcttcag tagaaccgtt accacgttcc
    19321 atacgcagac gagagtttgc gtgcagcggg gttaagttct caaagaggat tttgttgcgg
    19381 gcgttttcag gtttgtcgaa gttaacttcg ttaactttca gcagcgcaaa atagcgttca
    19441 ccttctttcg gcgggcgaat cttaccagag atggtatcac cagtgcggag gttgaaacgg
    19501 cggatttggc taggggaaac gtagatgtca tcaggaccgg cgaggtagga gctgtctgcg
    19561 gaacggagga aaccaaatcc atcctgcaat atctccagta caccatcacc aaagatatct
    19621 tcgccactct ttgcgtgctg cttcaggatg gcaaaaataa tgtcctgctt acgcatacga
    19681 gccaggtttt ccagccccat attttcgccg agagtgatca gctcagaaac cggcgtattc
    19741 tttaattcgg taagattcat agtggtgtga gttcttaaac ttggggtaaa tctcgaactt
    19801 aatgttgtga atggtatggc aggatcatcc atgcctgttt acggtcatcg actcatgtct
    19861 tttcgctgcc tggtcacagg agagtacgca gaactgaaac gacaagacgg attgagtgac
    19921 gagccagaaa tctgttcact tcgcatttaa gataaaacgg gaagcgttgg aaattacaag
    19981 attcaaactt aataaggtat gtttaatacg aagtcaacac taagttagca tgactcacgc
    20041 cgggcgtcca gtttttagcg acggggcacc cgaacatgaa attcccttac gccaggttag
    20101 cgtcgaggaa ctctttcaac tgacctttag acagtgcgcc cactttggtt gccgccactt
    20161 caccgttttt gaacagcagc aaagtcggga taccacggat gccatatttc ggcgcagtgc
    20221 cagggttttg atcgatgttc agttttgcaa cggtcagttt gccctgatat tcgtcagcga
    20281 tttcatccag aatcggggcg atcattttgc acggaccgca ccactctgcc cagaaatcga
    20341 cgaggatcgc cccgtccgct ttgagtacat ccgtgtcaaa actgtcgtca gtcaggtgaa
    20401 taattttatc gctcatatat aactccacag gaataagcct ggcgtgttgg tttcgttgtt
    20461 ggtgtaacat taaccaacta aaggttgact ttatttcacc ggatacgctt tcgtaaagca
    20521 atagtaagct gatattctac cacactatga gcaaaacaca tttaacagaa cagaagtttt
    20581 ccgacttcgc cctgcatccg aaggttgtag aagcccttga aaaaaaaggg tttcataact
    20641 gtacgcccat tcaggcactg gcccttccgc tgacgctggc gggtcgtgac gtagccgggc
    20701 aggcgcaaac cggtaccggg aaaacgatgg cgtttcttac gtcaacgttt cattatcttc
    20761 tctctcatcc cgcgattgcc gatcgcaagg tgaatcaacc gcgtgcctta attatggcac
    20821 cgacgcgtga acttgccgtg cagatccacg ccgacgcgga gccacttgcg caagctaccg
    20881 gcctgaagct gggtctggct tacggtggtg atggctacga caaacagctg aaagtgctgg
    20941 aaagcggcgt tgacattctg attggcacca cggggcgttt aattgactac gccaagcaga
    21001 accacattaa cctcggtgcc attcaggtag tggtactgga cgaagccgat cgtatgtacg
    21061 atctgggctt tattaaagat atccgctggc tgttccgccg tatgccgcct gcaaaccagc
    21121 gcctcaacat gctgttctcc gccacgcttt cgtaccgggt acgtgaactg gcgttcgagc
    21181 agatgaacaa tgccgaatat attgaagtgg aaccggaaca gaaaacgggc caccgtatta
    21241 aagaagagct tttctaccct tctaacgaag aaaaaatgcg tttgctgcaa acgctgatcg
    21301 aagaagagtg gccagacaga gcgattattt tcgccaacac caaacaccgt tgtgaagaga
    21361 tctggggcca cctggcggca gatggtcatc gtgtcggttt gttgacaggc gatgtcgcgc
    21421 agaaaaaacg tctgcgtatt cttgatgaat ttacccgtgg tgatttggat attctggttg
    21481 ccaccgatgt tgccgcgcgt ggcctgcata tcccggctgt gacgcacgtc tttaactacg
    21541 atttacccga tgactgtgaa gattacgttc accgtattgg tcgtacaggt cgcgctggcg
    21601 caagtggtca ttccatcagc ctggcatgtg aagagtatgc attgaatttg cctgctattg
    21661 agacctatat tggtcactca attccggtaa gcaaatacaa tccggacgca ttgatgaccg
    21721 atctgccaaa accgctgcgc ctcacgcgcc cgcgtacagg caatggtccg cgtcgtactg
    21781 gtgctccgcg taatcgtcgt cgttcaggtt aaataaaatg tcatactcaa aatcattcaa
    21841 actgtatcaa gatggcaaga gaatgaatcc cgatgcgcat gctgtagtaa gtgatttggg
    21901 tgaaagaacg cagccaacac agagacagat tgaaggatga agagtatggg ttccacctcg
    21961 tcgctgtatg cagccattga tctcggttcg aatagttttc atatgctggt tgtgcgcgag
    22021 gtggctggaa gcatccagac gctgacgcga attaaacgca aagtgcgtct ggctgctggc
    22081 ctgaatagcg aaaatgccct gtctaatgaa gcaatggagc gcggttggca atgtctgcgc
    22141 ctgtttgctg aacgtctgca agatatccct ccctcgcaaa ttcgcgttgt cgctacggcg
    22201 acattacgac tagccgtcaa tgcgggtgat tttatcgcca aagcacagga aatccttggc
    22261 tgcccggtac aagtcatcag cggtgaagaa gaagcccgcc tgatttatca gggggtggcc
    22321 cacacaacag gcggtgccga tcagcgcctg gtggtggata taggcggtgc cagtactgaa
    22381 ctggtaaccg gcacgggtgc acaaaccacc tcgttgttca gcctgtcgat gggctgcgtc
    22441 acctggctgg aacgctattt tgccgatcgt aatctggcgc aggaaaattt tgatgctgca
    22501 gaaaaagcgg cacgcgaagt gttacgtccg gttgcagatg aattacggta tcacggctgg
    22561 aaagtgtgcg ttggcgcttc cggcaccgtg caggcgttac aggaaatcat gatggcacag
    22621 gggatggatg aacgcattac cctggaaaag ttgcagcaac tgaaacaacg cgccattcat
    22681 tgcggtcggc tggaagagct agagattgac gggctgacgc tggaacgtgc gttagtgttc
    22741 ccgagtggtc tggcgatcct gatagccatt tttaccgaac tgaatattca gtgtatgacc
    22801 ctggcgggcg gtgcgctgcg tgaaggcctg gtctacggta tgttgcatct taccgtcgag
    22861 caggatattc gcagccgtac gctgcgtaat attcagcgcc gctttatgat cgatattgat
    22921 caggcacagc gcgtagccaa agttgcggct aacttctacg atcaggtgga aaatgaatgg
    22981 catcttgaag caataagccg cgatttgctc atcagcgcct gtcagcttca tgaaatcggc
    23041 ctgagcgttg acttcaaaca agcgccgcaa cacgctgctt atctggtacg taatctggat
    23101 cttcccggtt ttacccccgc acagaaaaaa ctgctggcga cgctactgct caaccagact
    23161 aatccggtcg atctctcatc gctgcatcag caaaatgccg taccaccgcg cgtcgcagaa
    23221 caactctgcc gtttactccg cctggctatc atttttgcca gccgtcgccg tgacgatctc
    23281 gtgccagaga tgacattaca ggctaaccat gaactgttga ccttgacgct tccgcaaggt
    23341 tggctaaccc aacatccgct gggtaaagag attattgatc aggaaagcca gtggcagagc
    23401 tatgtccact ggccgctgga agtgcattaa tgagtaagtg ccggatgcga tgctgacaca
    23461 tcttttccgg ccttgattat ttccctcgtt ttgccgccat catcgctttc agattcgcca
    23521 gatggctttg ccctttctgc atccgttctt cggcgctgac cactttgcgc tcctgttccc
    23581 aaatcaaatc atcctgcggc agctccagca ggaagcggct cggctccggg cgcaccagtt
    23641 ctccgtactg acggcgttct ttacacagcg taaaggtcaa ttccttctgg gcgcgggtaa
    23701 tgccgacata ggccagccgc cgctcctcat cgatattatc ttcatcgatg ctgctctggt
    23761 gcggcaaaaa cccctcttcc ataccgacca tgtagacata aggaaactcc agccctttcg
    23821 acgcgtggag agtcatcagt tgcacctgat ccagctcttc ttcactctca ccacgctcca
    23881 tcatgtcgcg caaagtaaag cgcgtcacca cctgggtgag cgtcatcggc tcatccagtt
    23941 cgctgccttc cagcatctcc gtcatccagc taaacagttg gttgacgttc ttcatgcgca
    24001 tttcggcggc tttcgggctg ggcgatgttt cgtacagcca ggattcataa tccatgccgt
    24061 ggatcagatc acgcaccgcg gcaatcggct cccgctccgc cagacgctgg atttctgcca
    24121 accagtgagt gaagcgggtc aatgcttcat aaccacgtcc gctaagcgtc tgactcaggc
    24181 ccatatcaaa gctggcggta aacatgcttt tattgcgcgt catcgcccat tcacccaact
    24241 ttttcagcgt cgccgggcca atctctcgct tcggcgtgtt aacgatacgc aggaacgcac
    24301 tatcatcgtc cgggttagtc agcacgcgca gataagccag caagtccttg atttcaggac
    24361 gagagaaaaa cgacgtacca ccagaaattt tgtacgggat gcggttttgc atcaggaatt
    24421 tttcaaacac ccgcgactga tggttaccgc gataaagaat ggcgtaatct ttgtactgcg
    24481 ttttattgac gaagtgatgg gcaatcagct cgccagtaac gcgctcggct tcatgttcct
    24541 cgttattcgc gcttaaaact tttagctctg tgccgtaacc cagttcggag aacagacgct
    24601 tttcaaagac gtgcgggtta ttggcgatca ggatgttcgc cgctttcaaa atacgtccgg
    24661 aagagcggta gttctgctca agcttaatca ccttcagcgc cggaaaatcc tgactcagca
    24721 gcaccaggtt ttgcggacgt gcgccgcgcc aggagtagat cgactggtca tcgtcaccca
    24781 ccacggtaaa gcgcgcgcgg ctgcccacca gcagtttcac cagctcatac tggctggtgt
    24841 tggtgtcctg atattcgtcc accagcaggt agcgaatttt gttctgccag cgctcgcgga
    24901 cttcttcatt gcgttgcaac agcaacgttg gcaataaaat cagatcatcg aaatcgagaa
    24961 cgttacaggc tttcaggtgt gcatcataca gcccataaca atgggcaaaa atacggtccc
    25021 gctcgccctt tgcctctgcc gccgcctgcg ccggtgtttt gagatcgttc ttccagttgg
    25081 agatggtcga aattagctgt tgcaggagaa ctttgtcatc ttcaatcagc ccctcggtca
    25141 actctttaag caaagcaagc tgatcggtat cgtcaaacag cgaaaagtta gccttcatcc
    25201 ccaacgccgc gtactcacgc ttaatgatat ccagccccag cgtgtgaaag gtggagatca
    25261 tcagcccacg cgcctcttta cgccccagcg tctgcccgac acgctctttc atctcgcgtg
    25321 ccgccttatt ggtaaaggtc accgccgcga tatgtcgcgc ctgataaccg caaccgcgga
    25381 tcagatgggc gattttattg gtgataacac gagttttacc ggaacccgcg cccgccagca
    25441 ccaggcaggg gccggtaacg aattcgacag cttgttgttg gccggggttt agacgcatag
    25501 gaaaatcgct caatgaaagt cgggagggga aaataacagc gtggtagtat agccagcctc
    25561 atccttacca ctcaaggcac gatcatggca aaaacagcag cagcactgca tatccttgta
    25621 aaagaagaga aactggctct ggatcttctc gagcagatca agaatggtgc cgatttcggc
    25681 aagctggcga agaaacactc catttgcccg tcaggcaaac gcggcggtga tttgggtgaa
    25741 ttccgccagg gtcaaatggt tccggcgttc gataaagtgg ttttctcttg tccggtactg
    25801 gagccaaccg gcccgctgca tacccagttc ggttaccaca tcattaaggt gctgtaccgt
    25861 aactaacagc aaggccttct ccgggagaag gccttgaact ctgaatcgct attaacccgc
    25921 aacagcaata cgtttcatat ccgtcatata gccgcgcagt ttcttaccta cctgctcaat
    25981 cgcatggctg cgaatcgctt catttacatc acgcagctgc gcgttatcta ccgcaccttc
    26041 cggaatagct ttacccaggt cgcccggttg cagctctgcc ataaacggtt tcagcagcgg
    26101 cacacaagcg taagagaaca gatagttacc gtactcggca gtatcggaga taaccacgtt
    26161 catttcgtac agacgcttac gggcgatggt gttggcaatc agcggcaatt cgtgcagtga
    26221 ttcatagtaa gcagattctt cgatgatgcc ggaatcaacc atggtttcaa acgccaactc
    26281 aacgcctgct tttaccatcg cgatcatcag tacgccttta tcgaagtact cctgttcgcc
    26341 gattttgcct tcatactgcg gcgcggtttc gaatgcggtt ttgccagtct cttcacgcca
    26401 ggtcagcagt ttcttatcgt cgttggccca gtcagccatc atgccggagg agaattcgcc
    26461 ggagatgatg tcgtccatat gtttctggaa cagcggcgcc atgatctctt tcagttgctc
    26521 agagagcgcg taagcacgca gtttcgccgg gttagaaaga cggtccatca tcagggtgat
    26581 gccgccctgt ttcagcgctt cggtgacggt ttcccaaccg aactgaatca gtttttctgc
    26641 gtatgccgga tcggtgcctt cttccaccag cttgtcgaag cacagcagag aaccagcttg
    26701 caacataccg cacaggatgg tttgctcgcc catcaggtca gatttcactt ccgcaacgaa
    26761 agaggattcc agcacgcccg cacggtgacc gccggttgca gccgcccatg ctttagcgat
    26821 cgccatgcct tcgcctttcg gatcgttttc cgggtgaacg gcaatcagcg tcggtacgcc
    26881 gaatccacgt ttgtactctt cacgtacttc ggtgccaggg catttcggcg caaccattac
    26941 gacggtgatg tctttacgga tctgctcgcc cacttcgacg atgttgaaac cgtgtgagta
    27001 acccagcgcc gcgccgtctt tcatcagtgg ctgtacggtg cgtactacat cagagtgctg
    27061 cttgtccggc gtcaggttaa ccaccagatc cgcctgcggg atcagttctt cgtaagtacc
    27121 cactttaaaa ccattttcgg ttgctttacg ccaggatgcg cgcttctcgg caatcgcttc
    27181 tttacgcaga gcgtaagaaa tatccagacc agaatcacgc atgttcaggc cctggttcag
    27241 cccctgtgcg ccacagccga cgatgactac ttttttaccc tgaaggtagc tcgcgccatc
    27301 ggcgaattca tcgcgcccca taaagcgaca tttgcccagc tgtgccagct gctggcgcag
    27361 attcagtgta ttgaagtagt tagccatggt gattcctcgt gatgttgtgc ttcttattgt
    27421 tcggttcgcg tttgcgagat tgaattcact atatgacagg aaatttattg cggaaattga
    27481 tatattcaca acgtcacatt gcaatttctg caacgtcaac attgagggct gcgccattgg
    27541 atttacgcga tctgaaaacc ttcctgcatc tggcggaaag ccgccatttt ggccgtagcg
    27601 cgcgggcaat gcacgttagc ccgtctacgc tttcgcgaca aatacagcgc ctggaagaag
    27661 atctcggtca gccgctgttt gtgcgtgata accgcacggt aacgctgacg gaagcgggcg
    27721 aagagctgcg cgttttcgcc cagcaaacgc tgttgcagta tcagcaattg cgccacacca
    27781 tcgaccagca agggccgtcg ctctctggcg aattacatat cttctgctcg gtgaccgctg
    27841 cctacagcca tctgccgccg attctggatc gcttccgcgc ggaacatccg tcagtggaga
    27901 tcaaactctc taccggtgat gcggccgatg cgatggaaaa ggtggtcact ggcgaagcgg
    27961 atctggcgat tgccgggaaa ccggaaacct tgccgggcgc agtggcgttt tcgatgctgg
    28021 agaatctggc ggtggtgctg attgctcccg cgctgccctg cccggtgcgt aatcaggtat
    28081 ctgtcgaaaa gcccgactgg tctacagtgc cgtttatcat ggccgatcag ggaccggtac
    28141 gtcgccgcat tgaactgtgg tttcggcgta acaaaatcag caacccaatg atttacgcca
    28201 cggttggcgg gcatgaagcg atggtatcga tggtggcgct cggctgtggc gtggcgctgt
    28261 tgccggaagt ggtgctggat aacagcccgg aaccggtgcg taaccgcgtg atgattttag
    28321 agcgcagtga tgaaaaaacg ccgtttgagc tgggcgtgtg cgtacaaaaa aagcggcttc
    28381 atgagccgct gattgaggcg ttctggaaga ttttgccgaa ccactaaccc gccaaaaaga
    28441 acctgaacgc cgggttattg gtttcgtcgt ggcaatcgta gcccagttca ttcagccggg
    28501 tttcaaaatc cggttcatga tcgccaagct cgaacgccgc cagtacgcgc ccgtagtcgg
    28561 taccgtggct gcgataatgg aacagcgaga tgttccagtg cgtacccagc gtgttgagga
    28621 agcgcagcag cgcgcccggc gattccggga attcgaagct gtagaggcgt tcctgcaacg
    28681 gatgcgatgg acgcccgcca accatatagc gcacatgcag cttcgccatt tcgtcgtcgg
    28741 agagatccac cacgctgtag ccgccatcgt tgagcatctg caaaatttct ttgcgctctt
    28801 cgaggccacg gctcaggcgc acgccgacaa aaatgcaggc gtttttggca tcagcaaagc
    28861 ggtagttgaa ctcggtgacc gagcgcccac caagtaactg gcagaatttg aggaagctgc
    28921 ccttttcttc cggaatggtc accgccagta acgcttcacg ctgttcgccc agttcgcagc
    28981 gttccgagac gtagcgcaga ccgtgaaagt tcacgttggc accggaaaga atatgcgcca
    29041 gccgctcacc gcgaatgttg tgctgggcga tgtatttttt catccctgcc agcgccagcg
    29101 cgccagaggg ttcagccacc gcgcgtacat cttcgaacag gtctttcatc gccgcacaga
    29161 tggcatcgct atcgacggtg atgatgtcgt caagatactc ctggcacaaa cggaaggttt
    29221 catcgccgat gcgttttacc gcgacgcctt cagcaaacag ccccacgcgg ggcagatcaa
    29281 cgggatgacc tgcatcaagt gccgctttca ggcaggcgga atcttccgct tctacggcga
    29341 tcactttgat ttgtggcatc agttgtttga tcagcaccgc cacgcccgcc gccagaccgc
    29401 cgccgccgac cggaacaaat acgcggtcga gatgagcatc ctgctggagc agttccagcg
    29461 ccagcgtacc ctgaccagcg atcaccatcg gatgatcgaa gggtggcacc caggtgaagc
    29521 cctgctgctg tgacagttcg attgctttcg ctttcgcttc gtcaaagttc gcgccatgaa
    29581 gcagcacttc gccgccaaag ccgcgcaccg catcaacttt gatatcggcg gtggcggttg
    29641 gcatgacgat cagcgccttc acgcctaacc gtgcggaaga aaacgcgacg ccctgcgcgt
    29701 ggttacccgc agaagcggta atcacgccgt gcgctttttg ttcttccgtc aggcccgcca
    29761 tcatggcgta tgcaccacga agcttaaagc tgtgcactgg ctggcgatct tcgcgcttca
    29821 ccagaatcac gttatcaaga cgcgacgaca gtttttccat tttctgtagc ggcgtgacct
    29881 gcgccgcttc gtagaccggt gcgcgcagca ccgctcttaa atattcggca ccttccgggg
    29941 caccggacag gggttgtgag tctgccatca ttaacccccc agtttcgatt tatcgcgcac
    30001 cgcgcctttg tcggcgctgg ttgccaggct ggcgtaggcg cgcagggcaa aggagacctg
    30061 acgttcacgg tttttcggcg tccaggcttt gtcaccccga gcttcctgcg cttcacgacg
    30121 cgccgccagt tcggcatcgc ttacctgtaa ctgaatacca cggttcggaa tgtcgatggc
    30181 gatcaggtcg ccgtcttcaa tcaggccaat gctgccgccg cttgccgctt ccggtgagac
    30241 gtggccgata gaaaggccag aggtaccgcc agagaaacga ccgtcggtga tcagcgcaca
    30301 ggctttaccg agacccattg atttcaggaa gctggttggg tagagcattt cctgcatccc
    30361 cggaccgcct ttcgggcctt catagcgaat tactaccaca tctccggcga caactttacc
    30421 gccgagaatc gcttctaccg catcgtcctg gctttcgtac actttcgccg ggccggtgaa
    30481 tttgaggatg ctgtcatcca cgcccgcggt tttaacgatg cagccgtttt ccgcaaagtt
    30541 accgtagagc accgccaggc cgccgtcttt gctgtaggcg tgttccagcg agcggataca
    30601 gccattggcg cgatcgtcgt ccagcgtatc ccaacggcaa tcttgcgaga atgcctgggt
    30661 ggtacggata cccgcagggc cggcgcggaa catatttttt accgcgtcat cctgggtcag
    30721 cataacgtcg tattgttcca gcgtttgcgg caacgtcagg ccgagcacgt ttttcacatc
    30781 acggttcagt aaccccgcgc gatccagttc gccgagaata ccgataacac caccagcacg
    30841 gtgaacatct tccatatggt atttctgggt gctcggcgca actttacaca gctgtggaac
    30901 cttgcgggaa agcttatcga tatcactcat ggtgaagtcg atttccgctt cctgcgccgc
    30961 cgccagcaga tgaagtacgg tgttggtaga gccgcccatc gcgatatcca gcgtcatggc
    31021 gttttcaaac gccgccttac tggcgatatt acgcggcagt gcactatcgt cgttttgctc
    31081 gtaataacgt ttggtcaatt caacaatgcg tttaccagca ttgaggaaca gctgcttacg
    31141 gtccgcgtgg gttgccagca acgagccgtt gcccggctgc gacaggccca gtgcttcggt
    31201 caggcagttc attgagttag cagtaaacat cccggagcag gaaccgcagg ttgggcaggc
    31261 ggatcgttca acctgatcgc tctgggagtc agaaactttc gggtctgcac cctggatcat
    31321 cgcatcaacc agatcgagct taatgatctg atcggacagt ttggttttcc cggcttccat
    31381 cgggccgccg gaaacaaaga tcaccggaat attcaggcgc agggaagcca tcagcatccc
    31441 cggggtgatt ttgtcgcagt tagagatgca gaccatggcg tcggcgcagt gagcgttgac
    31501 catatactca acggaatcag cgatcaattc gcgagatggc agtgaataaa gcatcccccc
    31561 gtggcccatg gcaatcccat catccaccgc aatggtgttg aactctttgg caacgccgcc
    31621 agccgcttca atttgttcgg cgaccagttt accgagatcg cgcagatgga cgtgacccgg
    31681 tacaaattgg gtgaacgagt tcacaaccgc gataatcggc ttaccgaaat cggcgtcggt
    31741 cattccggtg gcgcgccaca gcgcacgagc acccgccata ttacgaccat gagtggtggt
    31801 ggcggaacgg tacttaggca tactttattt actcccagtg tctgtctcgt aaatgggacg
    31861 gtgcgtgccg tcccattttt tgtatttatt gattaacttg atctaaccag ccccatttat
    31921 cttcggtttc gccagtgaag aggccgaaga aggcttgctg aatgcgtttg gtaaccgggc
    31981 cgcaacggcc ttcaccaacc tgaataccgt ctacactgcg cactggcgtg atttctgccg
    32041 cagtaccgga cataaacact tcatccgcca ggtacagtga ttcgcgcgac agcacctgct
    32101 cacgcacttc aattcccagc tctttcgcca gtttgatgat ggcgtcacgg gtaatacccg
    32161 gcagcgcgga tgaggtgaac ggtggggtga acagcacacc gtctttcact tcaaacaggt
    32221 tttcgcctgc gccttcagag atgtaaccgt tcacatccag cgcgatacct tcctgataac
    32281 cgtggcggcg tgcttcacta cccaccagca gggaagagag gtagttacca ccggcttttg
    32341 ccgcggttgg gatggtgttt ggtgctgcgc ggttccagga ggaaaccatc gcatcgatcc
    32401 cctgctccag cgcttctgcg cccagatacg ccccccacgg gaaagcggcg ataatcacgt
    32461 cggttgagta tcccgctggc gggtttacgc ccatgccaac atcaccaacg aagatcagcg
    32521 gacggatata ggcgctggtg agattgtttt tgcggatcac gtcacgacaa gcttccatca
    32581 gctcatcaat gctctgcgaa accgggaagc gatagatttt ggcggagtca tgcagacgct
    32641 gcatatgctc acgatggcgg aatacaaccg gtcctttgtg cgagtcgtag caacggatgc
    32701 cttcaaaaac cgaggtgcca tagtgcagcg cgtgcgacat cacatgcacc ttcgcgtctt
    32761 cccagcgaac catctcccca ttgaaccaaa tgtaatcagc tttcttcgtg gtcattttta
    32821 tattcctttt gcgctcaggc gcggatttgt tgtgatgtgg ttgtgctctg gcagatggca
    32881 acgtgtgcga cgtccaccag tttatttaac tgactaaaca gtaagtcgac cgaccgtggg
    32941 ctggcaacgg tcaattcgat atttatattt tgtgcatcgc tggcggcggc catattcatt
    33001 gaacagacgt ggaaaccacg atgacgcacc acgcgtaaaa cacgttctaa ggtttccgga
    33061 ttgaagcgag ccgatacatt gacctgatgt tgcatcatga taatttctcc aacatttctg
    33121 aattactggc accaggcggc accagcggcc agacgttctc aagttcgtcg attgagacat
    33181 gaagcaggta tggcccatca ctgttcagca tggtgtcgag tgccgcttca acctggtctt
    33241 tatgggtgat gtgttggcca gggatgccga aggcgctggc taacatgagg aaatcggggt
    33301 tatcggtaag ggtggtttcg ctgtatcgtt cctggaaaaa cagttgctgc cattgtcgaa
    33361 ccatccctaa ccgttggtta tcgagtaaga cgattttcaa cggtaactgc ttgcgtttta
    33421 cggtgcccag ctcttgcaca ttcatcatga aagagccgtc accggagata cagacgacag
    33481 tatcgttcgg tcgcgcgact tgtgcgccaa ccgccgccgg taaaccgaaa cccatggtgc
    33541 ctaagccgct ggaggtgatg aagttttccg ggcgagtgtg gacgatgtgc tgggcagccc
    33601 acatctggtg ctgccccaca tctgtggtca cgatgcaatc cgcagattta cgatccgaca
    33661 gttgttttaa caacaacggc gcgtagatag cgtcaccagg atggtcgtaa cgccaggcat
    33721 gttcatcacg cagttgcgcg cagtgttgct gccagtcatc gatatttaac ggctgctgta
    33781 atgctggtaa cagagcattt aaatcaccct gtaatgccac atgtgcctga cgcagcttgt
    33841 tcatttctgc cggatcgata tccatatgga taacgctggc gtgtggtgcg aaggtgttca
    33901 gtttgccggt cacccggtca tcaaaacgtg cgcccacggc gatcagtaga tcgcactcct
    33961 gcactgcgaa gttcgccgct ttggtgccgt gcatccccag catgcccaga tagtacggat
    34021 aatctgcttc tactgcgccc agccctttca acgtacaggt ggcaggcatt tttgtggcag
    34081 cgagaaattc acgcaaagcc gaaactgcct gcgccatgcc cacgccacca ccaacgtaca
    34141 gcatcggttt ttgcgctttt gccagcatct gacgcgcttg ctcgacttcg gcatgtggga
    34201 aagtcacttc gttttcaaca gtggtgaacc acggttccag atcgccgctg gctaactgga
    34261 tatcttttgg aatatcgacc aaaaccggac caggacgacc agagctggcg acgtcaaacg
    34321 cttcggccat gatacgcggt aactcttcca gtgattgcac caggaaactg tgcttggtgc
    34381 aggctaacga caaccccaga atatccacct cctgaaatgc gtcagtgccg ataaacggtg
    34441 cggatacctg accggtgata gcaacaacgg ggatggaatc taacagcgcg tccgcaagcc
    34501 cggttatcag gttggttgca cctggaccag acgtggcgat gcaaacgccc gttttgccgg
    34561 tagcacgggc ataaccgata gccgccattg ctgcaccttg ctcgtgtcgg cacagcaagt
    34621 gttccacgcc accgtcatac aatgcatcgt aaaccggcat aattgcgcca cccggataac
    34681 cgaaaacggt gttcacaccc tgtgcccgca acgcatgtac cacccactgt gcgccattca
    34741 tagttagttc cccgtcctga atcttgagaa acagaatttt gtgctgttat tcattgtctg
    34801 ctcctcagtt atgtttttaa ggtcaaaaaa aacccccgga cctttcggtg cgggggtctt
    34861 agttcgttaa ggcttgatct ctaagccttt cctcgtccaa gtgcagcccc gcacggtggg
    34921 ataataatca ccaccacgct aatcacgacc aggctaatca ctcgtagaag ggctgtcatt
    34981 ttgtcttttc ttgcatcttg ttcgaaggaa tgcctaaaga gttaccatag attttgcgaa
    35041 tagcacaaga tattttttgg ccgattttta tgatgtcttt aataatttca agagaaattg
    35101 ttgtttttta aaaagaaaat agtgagtgaa aaaaattcaa tttcagtatg aatgttgttc
    35161 ccttcctcgt agttcatcct ttgcccgctg catttttgcg agcatcattc caccgccaga
    35221 acaactcaac ttcacttgca caaataaaca ggaatcagcc actaaaattc acgaattagc
    35281 gggctacagc agcgatatct tatgcacata atggtgactc aaggagggct tatgtcactg
    35341 tcaattgttc atacccgcgc agccctggga gtaaatgcgc ccccaatcac tgttgaggta
    35401 catatcagta aaggtctacc aggcttaacg atggtgggct taccagaaac aacggtaaaa
    35461 gaagctcgcg atcgcgtgcg cagcgccatt atcaatagcg gatatgaata tccggcgaaa
    35521 aaaatcacca tcaaccttgc tccagccgat ctgccaaaag aagggggacg atatgatttg
    35581 cctatcgcta ttgcgttact ggcggcctca gaacagctta cagccaataa gttagatgaa
    35641 tatgaattag tcggagaact ggcgcttaca ggcgctctgc gtggcgttcc cggcgcaatc
    35701 tccagtgcaa ctgaagccat taagtcgggc agaaaaatta tcgtcgcgaa agataacgag
    35761 gatgaagtgg ggctaattaa cggtgaagga tgcctgatag ccgatcatct gcaggctgtc
    35821 tgtgcgtttc tggagggtaa gcacgctctc gaacgcccga aaccaactga tgcagtatcc
    35881 cgggcgctac aacatgatct cagtgatgtt gtcggtcagg agcaaggaaa gcgaggactg
    35941 gaaattaccg ccgctggcgg gcacaacctt ttactgattg ggccgccggg aacaggtaaa
    36001 acaatgctcg ccagccgtat taatggcctg ttgccagatt taagcaatga agaggcactg
    36061 gagagcgctg cgatattaag tctggtaaat gctgaatcag tacaaaaaca atggcggcag
    36121 cgcccattcc gctcacctca tcacagtgcg tcgttaactg cgatggtggg cggtggtgca
    36181 attccagggc caggtgaaat ttcgctggcg cataacggcg tgctttttct tgatgagcta
    36241 cctgaatttg aacggcgtac actggatgcc ttgcgagagc cgattgaatc cgggcagatc
    36301 catctttcac gcacacgagc aaaaataacc tatccagccc gtttccaact tgttgcggcg
    36361 atgaatccca gccctaccgg acattatcag ggaaaccata accgctgcac accagaacag
    36421 acattacgtt atctcaaccg gctctcgggg ccctttctcg accgcttcga tctctcacta
    36481 gagatcccat taccgccccc cggcattttg agtaaaacgg tagtgcaggg agaaaacagc
    36541 accaccgtta aacaacgtgt aatggccgcc agagagcgcc aatttaagcg gcagaataaa
    36601 ctgaacgcct ggctggataa tccggaaata cgccaattct gcaagcttga gagcgaagat
    36661 gcgcagtggc tggaagaaac gctgatccat ctggggttat cgattcgtgc ctggcagcgg
    36721 ttattgaaag ttgcacgaac cattgctgat attgatcagt ctgacattat cacacgtcag
    36781 catttgcagg aggcagttag ctatcgtgcg attgaccgtt tgctcatcca tctgcaaaaa
    36841 ctactgacat aaaaaaaagg gcatttcgcc ctttttatta atcgtcagaa tcggtgtagt
    36901 cttcagcacc ttcaacctgc ggtttaccgc cggaaagggt gtgaaaacgt tttggacgct
    36961 tgatacgcgt catatacttg gaccacacgc gttctgcttc tgtcactggc tcacgttcgc
    37021 cacggcatac tgctacgaag agtttctctt cctcggtaac cggctcgcgt ttgccaagat
    37081 ccaactcatt gaaggcataa ccatgacgct caagcagttg tgcctctttg atggtgaagt
    37141 caccatgacg agagaatcca cgtggataat gtttattgtc gaaatatcga ttagtcgtcg
    37201 taaagctttc cgccatcctg cacgctccta attctttgac cgagctagtt atggcgcgga
    37261 gtattagtta cgcttgacag agtgtaaaac aaaacattta aatcataacg acaaataatt
    37321 ttgcggagag cactgtggat acggaattgt taaaaacttt cctggaagtt agccgaacgc
    37381 gtcactttgg tcgagcagct gaatcgctct atctgaccca atcagccgtg agctttcgaa
    37441 tcagacaact ggaaaatcaa ctgggtgtga accttttcac ccgccacaga aacaatatcc
    37501 gtttaaccgc tgctggtgaa aaactactgc cttatgcaga aacgctcatg agcacctggc
    37561 aggccgcccg taaggaggtg gcgcatacct cacgacataa cgagttttct atcggtgcca
    37621 gcgcctcgtt gtgggaatgt atgcttaatc agtggctggg acgcttgtat caaaatcagg
    37681 atgtccatac aggcttacag ttcgaagcgc gaattgccca acggcagtct ctggtaaaac
    37741 agctgcatga acgccagctt gatcttctta ttactactga agcgcccaaa atggacgaat
    37801 ttagcagtca gttgctcggg tatttcactt tagcgcttta taccagtgcc ccttctaaac
    37861 taaagggaga tcttaactat ctgcgactgg aatgggggcc agattttcaa cagcatgagg
    37921 caggtttgat cggtgctgac gaagttccca ttctgacaac cagttctgct gaactggcac
    37981 agcaacagat tgcgacgctt aatggttgca cctggctacc cgtcagttgg gcgcgtaaaa
    38041 agggcggcct gcataccgtt gtcgatagca caacactttc acggccgctt tatgccatat
    38101 ggctgcaaaa tagcgataaa aatgcgttga ttcgcgatct attgaaaatt aacgtgctgg
    38161 atgaagtgta ttaatctgaa tggctggcaa ggatgccggt agaaggattt acttcggaga
    38221 ggttatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gcaggggcgg
    38281 agagactcga actcccaaca cccggttttg gagaccggtg ctctaccaat tgaactacgc
    38341 ccctaattag ggtggcggaa cggacgggac tcgaacccgc gaccccctgc gtgacaggca
    38401 ggtattctaa ccgactgaac taccgctcca ccgaattctt ttacaaccac cggttttatg
    38461 accggctt
//
LOCUS       DAZGHF010000028        38187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGHF010000028 DAZGHF010000000
VERSION     DAZGHF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.028"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(135..830)
                     /gene="aqpZ"
                     /locus_tag="ACHK3A_001602"
     CDS             complement(135..830)
                     /gene="aqpZ"
                     /locus_tag="ACHK3A_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO8739696.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAAAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1256..2914
                     /gene="ybjD"
                     /locus_tag="ACHK3A_001603"
     CDS             1256..2914
                     /gene="ybjD"
                     /locus_tag="ACHK3A_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO8739697.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2911..3867)
                     /gene="ybjX"
                     /locus_tag="ACHK3A_001604"
     CDS             complement(2911..3867)
                     /gene="ybjX"
                     /locus_tag="ACHK3A_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO8739698.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYKGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4018..5133
                     /gene="macA"
                     /locus_tag="ACHK3A_001605"
     CDS             4018..5133
                     /gene="macA"
                     /locus_tag="ACHK3A_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO8739699.1"
                     /translation="MKKRKTVKKRYVIALVIIIVGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5130..7076
                     /gene="macB"
                     /locus_tag="ACHK3A_001606"
     CDS             5130..7076
                     /gene="macB"
                     /locus_tag="ACHK3A_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO8739700.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7149..7373)
                     /gene="cspD"
                     /locus_tag="ACHK3A_001607"
     CDS             complement(7149..7373)
                     /gene="cspD"
                     /locus_tag="ACHK3A_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO8739701.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7523..7597
                     /gene="yljB"
                     /locus_tag="ACHK3A_001608"
     CDS             7523..7597
                     /gene="yljB"
                     /locus_tag="ACHK3A_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO8739702.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7696..8016
                     /gene="clpS"
                     /locus_tag="ACHK3A_001609"
     CDS             7696..8016
                     /gene="clpS"
                     /locus_tag="ACHK3A_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO8739703.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8047..10323
                     /gene="clpA"
                     /locus_tag="ACHK3A_001610"
     CDS             8047..10323
                     /gene="clpA"
                     /locus_tag="ACHK3A_001610"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO8739704.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10846..10933)
                     /locus_tag="ACHK3A_001611"
     tRNA            complement(10846..10933)
                     /locus_tag="ACHK3A_001611"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10897..10899),aa:Ser,seq:gga)
     gene            complement(11187..11405)
                     /gene="infA"
                     /locus_tag="ACHK3A_001612"
     CDS             complement(11187..11405)
                     /gene="infA"
                     /locus_tag="ACHK3A_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO8739705.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11690..12394)
                     /gene="aat"
                     /locus_tag="ACHK3A_001613"
     CDS             complement(11690..12394)
                     /gene="aat"
                     /locus_tag="ACHK3A_001613"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO8739706.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12436..14157)
                     /gene="cydC"
                     /locus_tag="ACHK3A_001614"
     CDS             complement(12436..14157)
                     /gene="cydC"
                     /locus_tag="ACHK3A_001614"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO8739707.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAALEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFNYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNGSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHNAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(14158..15924)
                     /gene="cydD"
                     /locus_tag="ACHK3A_001615"
     CDS             complement(14158..15924)
                     /gene="cydD"
                     /locus_tag="ACHK3A_001615"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO8739708.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(16047..17012)
                     /gene="trxB"
                     /locus_tag="ACHK3A_001616"
     CDS             complement(16047..17012)
                     /gene="trxB"
                     /locus_tag="ACHK3A_001616"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO8739709.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17556..18050
                     /gene="lrp"
                     /locus_tag="ACHK3A_001617"
     CDS             17556..18050
                     /gene="lrp"
                     /locus_tag="ACHK3A_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO8739710.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18185..22252
                     /gene="ftsK"
                     /locus_tag="ACHK3A_001618"
     CDS             18185..22252
                     /gene="ftsK"
                     /locus_tag="ACHK3A_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO8739711.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAAEQSAQQPYYAPA
                     PEQTAAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQHPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPHDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAHHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            22411..23022
                     /gene="lolA"
                     /locus_tag="ACHK3A_001619"
     CDS             22411..23022
                     /gene="lolA"
                     /locus_tag="ACHK3A_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO8739712.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSNFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            23033..24376
                     /gene="rarA"
                     /locus_tag="ACHK3A_001620"
                     /gene_synonym="mgsA"
     CDS             23033..24376
                     /gene="rarA"
                     /locus_tag="ACHK3A_001620"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO8739713.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24467..25759
                     /gene="serS"
                     /locus_tag="ACHK3A_001621"
     CDS             24467..25759
                     /gene="serS"
                     /locus_tag="ACHK3A_001621"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO8739714.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25998..28442
                     /gene="dmsA"
                     /locus_tag="ACHK3A_001622"
     CDS             25998..28442
                     /gene="dmsA"
                     /locus_tag="ACHK3A_001622"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO8739715.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28453..29070
                     /gene="dmsB"
                     /locus_tag="ACHK3A_001623"
     CDS             28453..29070
                     /gene="dmsB"
                     /locus_tag="ACHK3A_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO8739716.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            29072..29935
                     /gene="dmsC"
                     /locus_tag="ACHK3A_001624"
     CDS             29072..29935
                     /gene="dmsC"
                     /locus_tag="ACHK3A_001624"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO8739717.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29971..30597)
                     /gene="ycaC"
                     /locus_tag="ACHK3A_001625"
     CDS             complement(29971..30597)
                     /gene="ycaC"
                     /locus_tag="ACHK3A_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO8739718.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30912..32060
                     /gene="ycaD"
                     /locus_tag="ACHK3A_001626"
     CDS             30912..32060
                     /gene="ycaD"
                     /locus_tag="ACHK3A_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8739719.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32270..33700
                     /gene="ycaM"
                     /locus_tag="ACHK3A_001627"
     CDS             32270..33700
                     /gene="ycaM"
                     /locus_tag="ACHK3A_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO8739720.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33701..33811)
                     /locus_tag="ACHK3A_001628"
                     /pseudo
     CDS             complement(33701..33811)
                     /locus_tag="ACHK3A_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(33910..34650)
                     /gene="pflA"
                     /locus_tag="ACHK3A_001629"
     CDS             complement(33910..34650)
                     /gene="pflA"
                     /locus_tag="ACHK3A_001629"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO8739721.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMREVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34842..37124)
                     /gene="pflB"
                     /locus_tag="ACHK3A_001630"
     CDS             complement(34842..37124)
                     /gene="pflB"
                     /locus_tag="ACHK3A_001630"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO8739722.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(37179..38036)
                     /gene="focA"
                     /locus_tag="ACHK3A_001631"
     CDS             complement(37179..38036)
                     /gene="focA"
                     /locus_tag="ACHK3A_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO8739723.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9418 a         9331 c        10165 g         9273 t
ORIGIN      
        1 gcgccctcag cccacatcca tgtgggctgc cccggccttc ggggaacgct tcagcgattt
       61 tgacgccacc aacacccgtg cggtttgtaa cgtcgaagat aaaacaacca gggcagataa
      121 aatgccaatg ctaattaatt acgcttttcc agcagggtcc ggtaaatcag accaccgata
      181 ataccgccga caattggcac cacccagaag aaccacagtt gttctaacgc ccagccgccc
      241 tggaagatag cagccgcggt gctgcgcgcc gggttaacag aagtgttagt caccggaata
      301 ctgattaagt gaatcagggt taaggccaga ccaatagcga tcggcgcaaa accagcagga
      361 gcgaatttgt cggttgcgcc gtggatcacc agcaggaaac ctgcactcaa taccagttca
      421 actaccagcg cggaaagcat ggaataaccg cctggcgaat gctcgccata accgttagaa
      481 gcaaaaccgc tggctgccgc gtcaaaacct gttttaccac tggcaattaa atacagcagc
      541 gccgccgcaa caataccgcc gacaacctgg gcaattacgt agccaacgac ttcttttgcc
      601 gggaaacgtc cgccagccca taaaccaata gtgaccgccg ggttaaaatg accaccagaa
      661 atatgaccaa cagcaaaggc catcgtcaga acggtcagac cgaatgccag cgccacgccg
      721 gcaaaaccaa tgcctaattc cgggaagcct gcggccagta cggcactacc acagccacca
      781 aaaacaagcc agaaagtacc aaaacattca gctgctaatt ttctgaacat atccacctca
      841 attaaaaatt gaccctgtga aaaatatggt cgttttatag ggtcgtcgta aaaaagtgac
      901 gacggaaata atgcgcggct attttaaaaa cgaaggcgag tcattcacca gataaataaa
      961 tccagtaaat ttgatttaag gcaacagcgg gttgccccat atagtcattt gtctgattga
     1021 cagtgtagtg caagcgaaag atttaatcct ttaggcgtaa taaaaaataa tttatcatgc
     1081 taattatttg attttgttgt ttttgcagac ttatcagcaa gagggagtat aacgcgatta
     1141 ttcgctcatt tttcagacat ttgccatgct taaatgtgat gtcatcacgt attagcatgg
     1201 cctttcccgt tatactgcca gcgtaaagga taagtcacat atttctggag gggatatgat
     1261 tcttgagcgc gttgaaattg tgggttttcg cggtatcaac cgtttgtcgt tgatgctgga
     1321 acaaaacaac gtcctgattg gggagaacgc gtggggtaaa tccagcttgc tggacgcctt
     1381 aaccctgctg ctatcgccag aatcagatct ctaccatttt gagcgcgacg atttctggtt
     1441 cccgccggga gatatcaacg ggcgagaaca tcatctgcat attattttga ccttccgcga
     1501 atcgctgcca ggccgacatc gggttcgccg ctatcggccg ctggaagcgt gctggacgcc
     1561 atgcaccgat ggttatcacc gtatttttta tcgtctggaa ggggagagtg cggaagacgg
     1621 cagcgtgatg acactgcgca gttttctcga taaagacgga catccgattg atgttgagga
     1681 tattaacgat caggctcgtc atctggtgcg tttaatgccg gtgctgcgct tgcgtgatgc
     1741 ccgttttatg cgccgtattc gtaacggcac ggtgccaaat gtccccaatg tggaagtcac
     1801 cgcgcgccag ctcgatttcc tcgcccgtga attatcctca catccgcaaa atctctctga
     1861 tgggcagatt cgtcagggac tttccgcaat ggtgcaactt cttgagcatt atttttctga
     1921 gcagggagct ggacaggcgc gatatcgttt aatgcggcgg cgagccagca atgagcaacg
     1981 aagctggcgc tatctggata tcatcaaccg gatgattgac cgacctggag ggcgctcgta
     2041 tcgggttatt ttgctcggcc tgtttgctac tttattgcag gcaaaaggca cattgcgact
     2101 ggataaagac gcccgtccat tgttgctgat cgaagatcca gaaacccgtt tacaccccat
     2161 tatgctttca gttgcctggc atctgttgaa tcttctgccc ttgcagcgca ttgccaccac
     2221 caactcgggt gagttacttt cgttaacgcc ggtagagcat gtttgccgac tggtacgtga
     2281 gtcctcgcgc gttgccgcct ggcgtctggg gccgagtggc ttgagtaccg aagatagccg
     2341 acgcatatcc tttcacattc gttttaaccg tccgtcatcg ctgtttgctc gctgctggct
     2401 gctggtggaa ggggaaacgg aaacctgggt tatcaatgaa ctggcgcgtc agtgcggaca
     2461 tcattttgat gccgaaggaa tcaaggtcat tgagtttgcc cagtccgggc taaagccact
     2521 ggttaaattt gcccgccgaa tggggattga atggcatgta ctggtcgatg gcgatgaagc
     2581 agggaagaaa tatgccgcta cggtacgcag cctgttgaat aacgatcggg aagccgaacg
     2641 agaacattta acggcgttac cggcgctgga tatggaacat tttatgtatc gccagggatt
     2701 ttccgatgtg ttccaccgcg tggcgcaaat cccggaaaat gtaccgatga atctgcgcaa
     2761 aattatctcg aaagcgatcc atcgctcttc caaacccgat cttgccattg aagtggcaat
     2821 ggaggccgga cgtcgtggtg tggactccgt accgacgctg ctgaaaaaaa tgttctcacg
     2881 cgtgctgtgg ctggcgcgcg gtcgcgcgga ttaaccgcga aacatcgtgg ccatttgtgg
     2941 ctgaatagcg tcgagcatct catagcgccg acggtattca gcccgttttt tactggcgat
     3001 ttcggcaatc tcttttcgtg ctatctgtgc cggaaggcgg taatggcgtt cagcatcaca
     3061 tacgccgcca accgactccc agaacgcgtt gtaatcggca tggatcttgc cttctttatc
     3121 acgataacgc aggctgcggt aaatatgcgt ttcattgctg acggcaataa tctgctctac
     3181 ctgcaaacgt tgggcaaaca gacaggccgc ttccatcaca aggcgtttgg gaaatagccc
     3241 gtggcaggct ttcgtcgcat tctggatttc ctgatgtgga atttcccatt ttgcgccttg
     3301 cagtccgcca ataaacattg ttcttttccc cttatattca cacagggtaa acgtgatctc
     3361 tgccagagga ataccttcgc tgttgcggaa caggattgtg ctgtcacctt ctttatccat
     3421 tgagatcatc atggtcagct caagcgtgaa ctgctcgccg tttttgcctt ccagtttagc
     3481 cagttgcagc ccgggggtat tcaaatataa gctgaattct tccgccgaca tacacccgcg
     3541 gagtaacgca taatggtaac gtaacgcctc cagcaattgc ttacggctaa gattcgccgc
     3601 aaggtaaggg cgatgcagac gcacaggcag tcgcggctgg cgcgttaaca acacattgag
     3661 attgggccag tgggaaagtt cgttcatcca ctcaacgctt aaacgcggca taatcaacga
     3721 gcgcagcaaa aatttctggc gaaaactacg gcgatgccag aatttacccg gccgacactg
     3781 tccacgtgcc agactaagaa aaagtgacag gctgctgaga gattcagatg gcgtaaaggt
     3841 ccgttcagtt agctgcgaca tattcatgaa atcaatggtt atacatgacg tcgatttcac
     3901 cattgcgtat cttaaccaaa catcaatagt gtgattacta atgtaaattt tagggttttg
     3961 ttgatatttc gttgaagtta atgacccgga ttggcatatg gagtattcag aatatttatg
     4021 aaaaagcgga aaaccgtgaa gaagcgttac gttattgcgc tggtgataat catcgtcggg
     4081 ctgattacgt tatggagaat tcttaacgca cccgtgccga cttatcagac gctgattgtg
     4141 cgccccggtg atttacagca aagcgtgctg gcgaccggaa agctggacgc gctgcgtaag
     4201 gttgatgtgg gcgcgcaggt cagcggtcag ttgaaaacgc tgtcggtggc gattggcgat
     4261 aaagtaaaaa aagaccagct tttaggggtt attgatcctg aacaggctga aaaccagatt
     4321 aaggaggtgg aagcaacgct gatggagcta cgcgcgcagc ggcagcaggc ggaagcggag
     4381 ctgaaactgg cgcgggtgac gtattcccgt cagcaacgtc ttgcacaaac gcaagcggtt
     4441 tcacagcagg atctcgataa cgccgcgacg gagatggctg tgaaacaggc gcaaattggc
     4501 accattgacg cgcaaatcaa gcgcaatcag gcctctctcg atacggctaa aaccaatctc
     4561 gattacaccc gcatcgttgc cccgatggcc ggggaagtca cgcaaatcac cactctgcaa
     4621 ggccagacgg tgattgccgc acaacaagca ccgaacattc tgacgctggc agatatgagc
     4681 accatgctgg taaaagcgca ggtttctgaa gcggatgtaa tccacctgaa gccggggcaa
     4741 aaagcctggt ttacggtgct tggcgatcca ctgacgcgct acgaggggca aatcaaggat
     4801 gtgctaccga cgccggaaaa gattaacgac gctattttct attacgcccg ttttgaagta
     4861 cccaacccca atggtttact gcggctggat atgaccgcgc aagtgcatat tcagctcacc
     4921 gatgtgaaaa atgtgctgac gatccctctg tcggcgttag gcgatccggt tggcgataat
     4981 cgttataaag tcaaattgtt gcgtaatggt gaaacacgcg agcgtgaagt gacgattggc
     5041 gcacgtaacg ataccgatgt tgagattgtc aaagggctgg aagcgggcga tgaagtggtg
     5101 attggtgagg ccaaaccagg agctgcgcaa tgacaccctt gctcgaatta aaggatattc
     5161 gtcgcagcta tcctgccggt gatgagcagg ttgaggtgct gaagggcatc agcctcgata
     5221 tttatgctgg tgagatggtg gcgattgtcg gcgcttcggg ttccggtaaa tcgaccctga
     5281 tgaatattct cggctgcctg gataaggcca ccagcggcac ctatcgcgta gccggtcagg
     5341 atgttgccac gctggacgcc gatgcgctgg cgcaactacg ccgcgagcat ttcggcttta
     5401 ttttccagcg ttaccatttg ctttcgcatt taaccgccga gcagaacgtt gaagtacccg
     5461 ccgtctatgc cggtcttgag cggaaacagc ggctgcttcg cgcccaggag ttgctgcaac
     5521 gactggggct ggaagaccgt acagagtatt acccggcaca gctttcgggt ggtcagcaac
     5581 agcgcgtcag catagcgcgg gcattgatga acggcggtca ggtaattctt gccgatgaac
     5641 cgaccggcgc actggacagc cattctggcg aagaggtgat ggcgatcctg catcagctgc
     5701 gcgatcgcgg gcatacggtg attatcgtta cccacgatcc gcaggtcgct gctcaggccg
     5761 agcgggtgat cgaaattcgc gacggtgaaa ttgtgcgcaa tcctcccgcc attgaaaaag
     5821 tgaatgttgc tggcgggacg gaacctgttg tcaacacggc gtctggctgg cggcagtttg
     5881 tcagcggttt taacgaggcg ctgacgatgg catggcgggc gctggcagcg aataaaatgc
     5941 gtactttact gaccatgctg gggattatta tcggtattgc gtcggtggtt tccattgtcg
     6001 tggtgggtga cgccgccaaa caaatggtgc tggcggatat tcgttctatt ggtacgaata
     6061 ctattgatgt ctatcccggg aaagattttg gcgatgacga tccgcaatat cagcaggcgc
     6121 tgaagtacga cgacttaatc gccatccaaa aacaaccgtg ggtcgcctca gccacacctg
     6181 ccgtctcgca aaacctgcgc ctgcgttata acaatgttga tgtggctgcc agtgccaatg
     6241 gcgtgagcgg cgattatttt aatgtctatg gcatgacctt cagtgaaggg aacaccttta
     6301 atcaggagca gctgaacggt cgtgcgcagg tggtggtgct cgacagtaat actcgccgcc
     6361 agcttttccc ccataaagca gatgtggttg gcgaggtgat tctggtcggc aatatgcccg
     6421 ccagagtcat tggtgtggcg gaagaaaaac agtcgatgtt tggtagcagt aaagtgctgc
     6481 gtgtctggct accttacagc acgatgtccg ggcgagttat gggccagtcg tggctgaact
     6541 ccattactgt cagggtgaaa gaaggctttg acagcgctga ggcggaacag caactcacgc
     6601 gtttactttc actgcgccac ggtaagaagg atttctttac ctggaacatg gacggcgtct
     6661 tgaaaaccgt tgaaaagacc acacgtactt tacaactgtt tctgacgctg gtggcggtga
     6721 tttcgctggt ggtgggcggt attggtgtaa tgaatattat gctggtgtca gtgaccgagc
     6781 ggacgcggga aattggcatt cgcatggctg taggtgcgcg agcaagcgat gttttgcaac
     6841 agttcctgat tgaagccgta ctggtttgcc tggtcggtgg cgcgttggga ataacactgt
     6901 cactgttaat tgctttcacc ttacagcttt tcttacccgg ctgggagatt ggtttttcac
     6961 cgttggcgct gctgctggcg tttctctgct cgacggtcac cgggatttta tttggctggt
     7021 tacccgcacg aaatgcggca cgactggatc cagtagatgc tctggcacga gagtaatttt
     7081 tgagataaaa atgccagccg atcgggctgg cattttgcct ttaggatgta cacaatgaga
     7141 cagaagagct atgcgactgc cgcttctact tcgacgggca caataacact ggcgtgattg
     7201 ccttttggcc cctggtggac atcaaactga acggattgtc cagcttttag cgttctgtaa
     7261 ccatccatct gaatggtgga ataatgagcg aaaatatctt cgccgccgcc ttcagggcag
     7321 atgaaaccaa accctttggc attgttgaac cacttaacag tacccttttc catgcttcga
     7381 catccttcgc aaatcttata caagtaagat ggaataaacc ggggtcagag agggggctgt
     7441 tcaaaacctc gccaactcta gaaatacaat ttagagaatt agggcgagcc gtcaagcatt
     7501 tgacaggggc cagggggcag gtatgaatca aaaatttgaa gcagttaacg ctattgacag
     7561 gaatgtgaca gatgtcgctg atgccaacga tagatgatag ttatctatca tgtggagtag
     7621 attggtcagg caaataagct cttgtcagcg gcagggcgtt ctgccgataa ccgtaaccga
     7681 agatgataac tgacaatggg taaaacgaac gactggctgg actttgatca actggcggaa
     7741 gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata aagtgatatt agtcaatgat
     7801 gattatactc cgatggagtt tgttattgac gtgttacaaa aattcttttc ttatgatgta
     7861 gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg ggaaggccat ttgtggagtc
     7921 tttaccgctg aggttgcaga aaccaaagtg gcgatggtga acaagtacgc gagggagaat
     7981 gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc aggcataaaa attgggggag
     8041 gtgcctatgc tcaatcaaga actggaactc agtttaaata tggctttcgc cagagcgcgc
     8101 gagcaccgtc atgagtttat gaccgtcgag cacttgttac tggcgctgct cagtaaccca
     8161 tctgcccggg aggcgctgga agcgtgctct gtggatttgg ttgcgctccg tcaggaactg
     8221 gaagccttta ttgaacaaac cacacccgtt ctgcctgcca gtgaagagga gcgcgacaca
     8281 cagccgacgc tgagttttca gcgtgtactg caacgtgcgg tcttccatgt ccagtcctcc
     8341 ggtcgcaatg aggttaccgg tgcaaacgtt ctggtcgcta tctttagcga acaggagtcg
     8401 caggcggcat atctgttgcg caaacatgaa gtcagccgtc tcgatgtggt gaactttatc
     8461 tctcatggca cgcgtaaaga cgagccgaca cagtcttctg atcctggcag ccagccaaac
     8521 agcgaagaac aagctggtgg ggaggaacgt atggagaatt tcacgacgaa cctgaatcag
     8581 cttgcgcgag tgggcggaat cgacccactg attggtcgtg agaaggagct ggagcgtgct
     8641 attcaggttc tctgccgtcg ccgtaaaaac aacccgctgc tggtggggga atctggtgtc
     8701 ggtaaaaccg cgattgcgga ggggcttgca tggcgaattg ttcagggcga tgtgccggaa
     8761 gtgatggctg actgtacgat ttactctctc gatatcggtt ctctgttagc gggcacaaaa
     8821 tatcgcggcg actttgaaaa acgttttaaa gcgttgctca agcagctgga gcaagacact
     8881 aacagcatcc tgtttattga tgagatccac accattatcg gtgcgggtgc agcgtctggt
     8941 ggccaggtcg atgcagctaa cctgatcaaa ccgttgctct ccagcggtaa aattcgcgta
     9001 attggttcga caacctatca ggagttcagc aacattttcg agaaagaccg tgctctggcg
     9061 cgtcgcttcc agaaaattga tattactgaa ccgtcaatcg aagaaactgt tcaaatcatc
     9121 aatggcctga aaccgaagta tgaagcgcac cacgacgtgc gttataccgc aaaagcggtg
     9181 cgtgcagcgg tagagctggc ggtgaaatac attaacgatc gtcatctgcc ggataaagcc
     9241 attgacgtta tcgacgaagc gggcgctcgc gcacgcctga tgccggtaag caaacgcaag
     9301 aaaaccgtta atgtggcgga tattgagtcc gtggtggccc gtattgcgcg cattccagag
     9361 aagagtgttt cgcagagtga ccgcgatacc ctgaaaaacc tcggcgatcg cctgaaaatg
     9421 ctggtcttcg gtcaggataa agccattgag gcgctgactg aagccattaa gatggcgcgt
     9481 gcaggtttag gtcacgaaca taaaccggtc ggttcgttcc tgtttgccgg tcctaccggg
     9541 gtcgggaaaa cagaggtgac ggtacagctt tcgaaagcgt tgggcattga gcttctgcgc
     9601 tttgatatgt ccgagtatat ggaacgccat accgtcagcc gtctgattgg tgcgcctccg
     9661 ggatacgttg gttttgatca gggcggtttg ctgactgatg cggtcatcaa gcatccacat
     9721 gcggtgctgc tgctggacga aatcgagaaa gcgcacccgg acgtgttcaa tattctgttg
     9781 caggtgatgg acaacggtac gctgaccgat aacaacggac gcaaagcgga cttccgtaac
     9841 gtggtattgg tgatgaccac caacgctggg gtacgagaaa ctgagcgtaa atcgattggt
     9901 cttatccacc aggacaacag taccgatgcg atggaagaga tcaagaagat ctttacgccg
     9961 gagtttcgta accgtctcga caacattatc tggttcgatc atctctccac cgacgtgatc
    10021 catcaggtag tggataaatt catcgtcgag ttgcaggttc agctggatca gaaaggtgtt
    10081 tctctggaag tgagccagga agcgcgtaac tggctggccg agaaaggtta cgaccgggca
    10141 atgggcgcac gtccgatggc gcgtgtcatc caggacaacc tgaaaaaacc gctcgccaac
    10201 gaactgttgt ttggttcgct ggtggacggc ggtcaggtga cggttgcgct ggataaagag
    10261 aaaaatgagc tgacttatgg attccagagt gcacaaaagc acaaggcgga agcagcgcat
    10321 taatctaatt gttaggtagg ttggtgaagt ccgtaatctc gaaagaggtt acggactttt
    10381 tgtttatggg gtggaggagg ttctgaccct ttttttgatg atgatggtaa tttgttgata
    10441 attagtgctg cgggaaggta aggataaaaa agggtggtag caggaaaatg agatggtttt
    10501 gctttattaa caacgggcta gacgtgtagt atttgagttc actgccgtac aggcagctta
    10561 gaaagccaac cgaaagaaaa actcgatttt tataccgttc actgccgtac agacagataa
    10621 aatgcgaaaa aaaaaacgta ctttcgtacg agctcttctt tgaatatggc ggtgaggggg
    10681 ggattgactc gcttcgctcg ccctgcgggc agcccactca ctgtgttcgt ggtctgtcca
    10741 actggctgcg ccagttgtcg aaccccggtc ggggcttctc atcccccctg gagtgcaata
    10801 tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt gaatatggcg gtgagggggg
    10861 gattcgaacc cccgatacgt tgccgtatac acactttcca ggcgtgctcc ttcagccact
    10921 cggacacctc accaaattgt tttgctacca aacctcatgg gtggcaacgg ggcgctacta
    10981 tagggagttg gagtaaaacg gtcaagaaga attttaatga taattattgt ttgctcatac
    11041 tgtaaacaag ttgtgcagta tatctacatc gagacaagtt atggacttat acttccaaag
    11101 tacttcatac atatcacaaa ataaaaaggc cggctaaacc gaccttttac tcgttctttc
    11161 tcttcgccca tcaggcggta aaacaatcag cgactacgga agacaatgcg gcctttgctc
    11221 aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg tcaggatgcg gatgtagttt
    11281 ttgcgcattt taccggagat gtgtgcagta accacgtgac cgttttctaa ctctacgcgg
    11341 aacatggtat taggcaacgt ttcaagaacg gtaccttgca tttcaatatt gtcttctttg
    11401 gccatctaat cctctggggt atcactaccg taatttgaac cggcaagata atgccgaagt
    11461 tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa acaggttcgg cacattactc
    11521 cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat aagggagcgg tgagataaac
    11581 gatgggcgtt acctgacgcg aaaaattcct tatcggcagc ggggtaatga gcgtaaccaa
    11641 ctctgcgacc gcaattataa cactctgggg agaaatgtgc cgaaaacatt cattcttgtg
    11701 gtgaaaacaa gcaccgtggt acccagaaat tattcggcaa tcgtccgagg cgcatttgat
    11761 tgagataatt gaggtaatcc cggcggggaa tttcgcaggc accaagcgac gctgtgtgat
    11821 cgttaaggac ctggcagtcg ataagtttac cgccatgacc aataaattcc tcacagaata
    11881 ccagaagcgc cgttttagac gcattttcca tccggctgaa catggactcg ccacaaaata
    11941 gcgttccctg ggccacgccg tacataccgc cgacaagctc atcttcacgc cagacttcaa
    12001 tagagtgggc atgaccgagt tcgtgaaggc gatggtaggc ttcgaccacg ccacgcgtga
    12061 tccaggttcc ttcttcgcga tcgctggcac agccttcaat gacctgacca aaagcgtaat
    12121 tcatcgtgac tcgatagggc gagcgtttat gaaatcgctt catactacgg ctgatatgca
    12181 gtgattcagg ccaaagcacc gcgcggggat cgggcgacca ccagaggatg gggtcacctg
    12241 gagaaaacca cggaaaaata ccacgctggt aagccattaa caggcgcgca gggctaagat
    12301 cgcctcccag tgccagcagg ccgttaggct cacgtaatgc gccttccggg gaagggaagg
    12361 ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc aaaactccac gcaagtcgga
    12421 tcgttcaata atagcttaca aaccctgctt aaactggtaa taacgcccct gtctggcaag
    12481 cagttctgcg tgagtacctt gctcaataat ttgcccgttg tccatcacta ttatttgttg
    12541 gaaacgagag agtccgcgaa gtcgatgggt gaccattaac accgttttct catgcatcat
    12601 ttctgcaagc aattcaagga tctgactttc ggttgtggca tctaagcctt ctgtaggttc
    12661 atccagcaat accagcggcg cattatgtaa cagcgcacgg gcgatagcca gacggcgtag
    12721 ttcaccaccg gagagctggc gtccgccttc acccaaccaa ctgttgagac ctgcatcctc
    12781 gagcagcttc tccagaccaa cgcgacgtaa gatctccgcc agagcctcat cactactgcc
    12841 aggcgaggcg agtaacagat tatcacgcag cgtggcgcta aacagatgca ctcgctgagg
    12901 aacaacgctg atggtctgtc gtagagtcgc ttcattcagg ctggctatgg ggctaccgtt
    12961 aagcaaaatc tcgccctgtt gcgggtccca tgcgcgggtc agcagttgta acagtgttga
    13021 tttgccgcat ccggttcgcc cgagaatcgc tatatgttcc ccggcgttta cctgaagaga
    13081 aatcccttta agtgcctgtt gggattgctc cggataattg aattgaacat cccgtaacgt
    13141 cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg aaggtgactt ccggtttttg
    13201 atccgttaag tcagtgatcc gtacggcaga ggcaatgact tgccccagat gctgaaatgc
    13261 acccgttact ggtgccagtg cttcaagcgc ggctaacgcg cagaagacaa acagggcaat
    13321 taacgcgccg ggttgagcat tgccgccaac gccgccagac gccatccaca gcatcaggat
    13381 caccgctaac gcgccaatga gcagcattat cgcttgcgac aatgcggtga gttcagattg
    13441 acgacgttgc gcttccagcc actgaatttc tgtattctct aattgcgtgc gataacgatc
    13501 gctggcacca aagatggtca gctcagcttg cccttgcagc caggccgtca gttgttggcg
    13561 atactgcccg cgaagatgag tcagattttg cccggtgctt tttcccgcac gataaaacag
    13621 tggtggcata aggaaaagcg tcagtaacat aatgccgccc agcgtaaagg cgagggtgaa
    13681 atcaaggaaa cttaacccga ttgtcaccac cataatcacc acaaaagcgc ccaccagcgg
    13741 cgagataacg cgcaggtaaa gatgatcgag cgtatcaaca tccgccacca cgcggttgag
    13801 caattcgccc tgacgatagc gcgccagtcc ggcaggggat aggggcagca atttgctgaa
    13861 ggtgtaaatg cgcagatgct gcaacacgcg gaaagtcgcg tcgtgactta ccagacgttc
    13921 aaaatagcgc ccggcagtac gggtgattgc tgcgccacgc acgcccgcag cgggtagcat
    13981 atagttgaag ctgtacagtc cggcaacccc cgctaccgct gaggcggaga ggaaccagcc
    14041 ggaaagtgtc aacagaccga tactggcgag cagcgtgaca attgccagca caataccaag
    14101 acttaacatc catttatgac gtttatacag tgccagatag ggtagcaaag cgcgcattta
    14161 aatctcctcc tgacgatggg ccagcaatgt ggcgaaagga ccaccagcca cgcttaattc
    14221 cgcgtaacgt ccttgctcaa taatctgacc atcctgcatt acccaaatga catcccagtc
    14281 agcaagatct tctaactggt gggtgaccat taacgttgtc tggcgcagag aggcggcatt
    14341 aagcgcctcc attacgcgct gttcactgtg agcatcaagg ctggcagcgg gttcatccaa
    14401 cagtaatagc gaacagggat ttagtaatgc tcgggccacc gccacgcgct gcgcctgccc
    14461 cacggaaagg cgggcagcct ggtcgccaac aggcgtatca acgccttgtg gcaggagcgg
    14521 tagaaactcg ctgacccagg cgttatccag cgctgtttgt aactcgtgtt cgctggcatc
    14581 aggtcgtgcc agtagtacgt tatcccgtag tgttgctgcc ggtaattgtg ggttttgccc
    14641 aacccaggag agatgcttac gccatgattc cggtgataaa tcgcgtaatt ctatcccgtt
    14701 gattcgtagc gatccctgat atgagagaaa accagaaagc gcgttcagca gtgagctttt
    14761 acctgaaccg ctgcgaccaa ccaacactgc acgttggcct gctggcaaag taaagttcag
    14821 cggtccagcc agcgttttac cttctggtga tgtaataaac agatcctcgg cttcaatggt
    14881 caccggatcg gttgatgcta attccgcctc accacgttgc ggatgggcta gcggggtttc
    14941 cataaacgtt ttcagactgt cagctgcgcc aacagcctgg gctttagcat gataaaacgt
    15001 accgagatcg cgtaatggct ggaaaaactc cggcgcaagg atcagggcca gaaaacccgc
    15061 agccagcgtc acgccagtat catagtgacc aaaatccagc tcgccgagat aggaaaaacc
    15121 aaagtagacc gccaccagag caattgacag cgaggtaaaa aattcgagaa tgccggagga
    15181 taaaaacgcc agccgtagca cttccattgt ccgttggcgg aaatcttccg aagcagaacg
    15241 aatactttca atttcagctt caccacgacc aaaaatacgc aatgtttcca tgccgcgcag
    15301 gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga aagttacgtc ggttagcatc
    15361 ggcagccccc attccaacca gcgccataaa caacggaatt aacggtgcag tgcccagcag
    15421 aataagcgcc gcagcccagt tagacgggaa gatcgccacc acaatcagca aaggcacgga
    15481 cactgccagc gccatttgcg gcaggtagcg tgcatagtaa tcatgcatat cgtcaatttg
    15541 ctcgagtacc agcgtcgccc agctccccgc cggtttaccc tgaatccacg ctggccctgc
    15601 ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag cggatatgct gcccggcgtg
    15661 ataacccacc cgttcgcgta accagaccac ccatgcgcgc agtacaaagg tcagaaccag
    15721 taacgtaaag ggaagcagca gggcttcacg gggaatattc tccataatca tatgttgcag
    15781 aattcgcgcc atgaaccagg cctgggcaat gatcaatatg ccgctcacaa agcccagcag
    15841 acgagaaata ttcagccaac gttgggagat gacgctttgc tgttttaacc agcgggttaa
    15901 ctctttttga cgggatttat tcattgcacg cttagcaggt gagttatcag aattatttgc
    15961 agagcaatgt tacaacgagg aaaaaataaa ggcgacccat agtcgcatgg tgtcgccttc
    16021 tttacttttg ttactgattt gtaaaattat tttgcgtcag ctaaaccatc gaggtagcgt
    16081 tccgcatcaa gtgctgccat gcagcctgta ccagcagatg taatagcctg gcgataaatg
    16141 tgatccatca cgtcgcctgc ggcaaagacg ccagggatgc tggtctgggt ggcattacca
    16201 tgaatacccg actgtacttt gatgtagccg ttttccagtt ccagctgccc ttcgaaaata
    16261 gccgtattcg ggctgtgacc gatagcaaca aacagaccgg caacgtcgag tgactcgatg
    16321 ttatcgctgt tttgcgtatc gcgcagacga acgccagtga cgcccatctg atcgccggtc
    16381 acctcttcca gcgtacggtt ggtgtgtaga atgatgttgc cgttctccac tttatccatc
    16441 agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac ggcggtgaat cagatgcact
    16501 tccgaagcga tgttagacag atacagcgcc tcttcaaccg cggtattgcc gccgccgatg
    16561 accgcaactt tctggttgcg atagaagaaa ccgtcgcagg tagcacaagc agaaacccca
    16621 cggcctttaa aggcttcttc agatggcagg cctagatagc gtgcagaagc tccggtggca
    16681 ataatcagcg cgtcgcaagt gtattcgccg ctatcgccag tcagacggaa cggacgattc
    16741 tgcaaatcaa ctttgttgat gtgatcaaag atgatttcag tttcaaattt ggtggcatgt
    16801 tcgtgcatgc gctccattaa taacggaccg gtcagatcgt ttggatcgcc aggccagttt
    16861 tccacttccg tggtggtggt cagttggccg cctttttcca tgccggtaat cagcacaggt
    16921 tgcaggttgg cgcgcgccgc gtagacagca gcggtatatc ccgccgggcc tgaacccagg
    16981 ataagcagtt tactgtgttt ggtcgtgccc atgagatccc catagttgtt ggcagacaat
    17041 gggcaggatt gtagggaatt tacagacgta aaaaaagagt atgacgattt tgttaacaat
    17101 ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc cgtcattatc acctctgcta
    17161 cttaaatttc ccgctttata agccgattaa atgatgaata aacgcccctg ttaatgaata
    17221 tctggcatgt tgtactaaaa atcgatgttt tgctttgaca atcccctggt gttttgcgaa
    17281 aacattcgag gaagaaaaaa acagtattct tatatgcgca taaccatgca tgtaaatacc
    17341 atgtttaccg tgctagtgaa atctacgtat ggcgtggaca gacgccattc gtgatgtcga
    17401 tagctgccac aaggcaacgg tcttctcacc gtagacccag gcattgcgcg ccgtgaatct
    17461 tcatgatttc ggtctatcgt gacgggtagc gactctgaac agtgatgttt cagggtcaga
    17521 caggagtagg gaaggaatac agagagacaa taataatggt agatagcaag aagcgccctg
    17581 gcaaagatct cgaccgtatc gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta
    17641 tttctaacgt cgagctttct aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg
    17701 tgcgtcggct ggaaagacaa gggtttattc agggctatac ggcgctgctg aacccccatt
    17761 atctggatgc atcacttctg gtattcgttg agattactct gaatcgtggc gcaccggatg
    17821 tgtttgaaca attcaatacc gctgtacaaa aacttgaaga aattcaggag tgtcatttag
    17881 tatccggtga tttcgactac ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta
    17941 agttactggg ggaaaccctg ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg
    18001 ttatggaaga agtcaagcag agtaatcgtc tggttattaa gacgcgctaa cacggaacag
    18061 gtgcaaaatc ggcgtatttt gattacactc ctgttaatcc atacagcaac agtactgggg
    18121 taacctggta ctgttgtccg ttttagcatc gggcaggaaa agcctgtaac ctggagagcc
    18181 tttcttgagc caggaataca ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg
    18241 ccgccgcctt ctggaggcgt tgctgatcct tattgtcctg tttgccgtct ggttgatggc
    18301 tgccttacta agctttaacc cttcggaccc cagctggtcg caaacggcct ggcatgaacc
    18361 tatccataat ttaggtggga tgcccggtgc gtggctggca gatacgctgt tctttatttt
    18421 tggcgtgatg gcttacacca ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg
    18481 tcatcagtcc agcgacgaat acattgatta ttttgccgtt tcgctacgca tcattggcgt
    18541 tttggcgctc atccttacct cctgtggtct ggcggcaatc aacgctgacg atatctggta
    18601 ttttgcctcc ggtggcgtca ttggcagctt actaagcact acgctacaac cactgctaca
    18661 cagtagcggg ggaactattg cgctgctctg cgtttgggct gcgggtctga cgctgttcac
    18721 cggttggtca tgggtgacca ttgctgaaaa actcggcggc tggattttaa acattctcac
    18781 cttcgccagt aatcgtaccc gtcgcgatga tacctgggtc gatgaagatg agtatgaaga
    18841 cgacgaagaa tatgaagatg aaaatcacgg taaacagcat gaatcacgcc gtgcccgtat
    18901 tcttcgcggc gcgttagcgc gtcgtaaacg gttggcggaa aaattcatta atccgatggg
    18961 gcggcaaaca gatgctgcgt tgttctccgg taagcggatg gatgatgaag aagagattac
    19021 ctacactgca cgcggtgtgg ctgccgaccc tgacgacgtc ctcttttcgg gcaatcgtgc
    19081 aacgcagcca gaatatgacg aatacgatcc attattaaac ggtgcgccaa ttaccgaacc
    19141 tgttgctgta gcagctgctg ctaccacggc gacacaaagc tgggctgcgc cggttgaacc
    19201 tgtgactcag acgccgcctg ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc
    19261 ctggcagcct gtaccgggtc cacaaacggg agagccggtg attgctccgg caccggaagg
    19321 ttacccacag cagccacaat atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca
    19381 acctgtacag ccacagcagc cgtattatgc gccagcagcg gaacaacctg tgcaacaacc
    19441 ttactacgct cctgcggcgg aacaacctgt tcagcagccg tattatgcaa ctgcggcgga
    19501 acagtctgcg caacagccgt attacgcccc tgcgccagaa caaacggcgg caggtaacgc
    19561 ctggcaagcc gaagagcagc aatccacttt tgctccacag tctacatacc agactgagca
    19621 aacttatcag cagccagccg ctcaggagcc gttgtaccaa cagccgcaac ccgttgaaca
    19681 gcaccctgtt gtggagcctg agcccgttgt agaagagaca aaacccgcgc gtccgccgct
    19741 ttactatttt gaagaagtgg aagagaagcg tgcccgtgaa cgtgaacaac ttgcggcctg
    19801 gtatcaaccg attcctgaac cggttaaaga accagaaccg atcaaatctt cgctgaaagc
    19861 accttctgtt gcagcagtac ctccagtgga agccgctgcc gctgtttctc cgctggcatc
    19921 tggcgtgaaa aaagcgacac tggcgacggg ggctgccgca accgttgccg cgccagtctt
    19981 cagtctggca aatagcgctg gaccgcgtcc gcaggtcaaa gaggggattg gcccgcagtt
    20041 gccacgaccg aaacgtatcc gcgtgccaac tcgtcgtgaa ctggcgtctt acggtattaa
    20101 gctgccctca cagcgtgcgg cagaagaaaa agcccgtgaa gctcagcgca atcagtacga
    20161 ttccggcgat cagtataacg atgatgaaat cgatgcgatg cagcaggatg aactggcacg
    20221 tcagttcgcc cagacacagc agcaacgcta tggcgaacag tatcaacatg atgtgcccgt
    20281 gaacgcagaa gatgcagatg ctgcggcaga ggctgaactg gctcgtcagt ttgctcaaac
    20341 tcaacaacaa cgttattccg gcgaacaacc tgctggggcg aatccgttca ctctggatga
    20401 ttttgaattt tcgccaatga aagcgttgct ggatgatggt ccacacgaac cgctgtttac
    20461 gccaattgtt gaacctgtac agcagccgca acaaccgatt gcaccgcagc aacagtatca
    20521 gcagccgcag caaccagttg cgccgcagcc gcagtatcag cagccgcaac agccggttgc
    20581 accgcagcag caatatcagc agccacaaca accggttgca ccgcagccgc aatatcagca
    20641 gccgcaacag ccggttgcac cacagccgca atatcagcag ccgcaacagc cagttgcgcc
    20701 gcagccgcat gataccctgc ttcatccgct gttgatgcgc aatggtgaca gccgtccgtt
    20761 gcataaaccg acgacgccgc tgccttctct ggatttgctg acgccgccgc cgagcgaagt
    20821 ggagccggta gatacctttg cgcttgaaca aatggcgcgc ctggtggaag cgcgtctggc
    20881 tgatttccgt attaaagccg atgtcgtcaa ttattctccg gggccggtta tcactcgctt
    20941 tgaattgaac ctggcaccgg gagtaaaagc ggcgcgtatt tctaacttgt cacgtgacct
    21001 tgcccgttca ctttcgacgg tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta
    21061 tgtagggctg gagttaccga ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga
    21121 taacgccaaa ttccgcgata atccgtcgcc attaaccgtg gtgctgggta aagatatcgc
    21181 cggtgagccg gtggttgccg atctggcgaa aatgccgcac ttgttggttg cggggactac
    21241 cggttccggt aagtctgtcg gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca
    21301 gccagaagat gtgcgtttca tcatgatcga cccgaaaatg ctggagcttt cggtttatga
    21361 aggcatcccg catctgttaa cggaagtcgt tactgatatg aaagatgccg ccaacgcgct
    21421 gcgctggtgt gttaacgaga tggagcgtcg ttataaactg atgtctgcgc tgggtgtgcg
    21481 taatctggcg ggttataacg aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc
    21541 agacccgtac tggaagccgg gtgatagtat ggatgcgcat catccggtgc tgaaaaaaga
    21601 accgtacata gtggtgttgg tggacgaatt tgccgacctg atgatgacgg taggtaaaaa
    21661 agtggaagag ctgatagcac gtctggcgca aaaagcccgt gccgcgggta tccacctcgt
    21721 actggcaacg cagcgtccat cggttgatgt tattactggt ctgattaaag cgaatattcc
    21781 gacccgtatc gcctttaccg tatccagtaa gattgactca cgtaccattc ttgatcaggc
    21841 tggcgcggaa tcactgctgg gtatggggga tatgctctac tctgggccga actccacgtt
    21901 gccggtacgt gtccatggtg cttttgtacg cgatcaggaa gttcatgccg tggtgcagga
    21961 ctggaaagcg cgtggtcgcc cacagtatgt tgacggcatc acctccgaca gcgaaagcga
    22021 aggtggtgcg ggtggtttcg atggcgctga agaactggat ccgttgttcg atcaggcggt
    22081 gcagtttgtc actgaaaaac gcaaagcgtc aatttcaggc gtacagcgtc agttccgcat
    22141 tggttataac cgtgcagcgc gtattatcga acagatggaa gcgcaaggga ttgtcagcga
    22201 acaggggcac aacggtaatc gtgaagtgct ggccccaccg ccgtttgact aattaatgca
    22261 tcgtaggccg gataaggcgc ggtagcgtcg catccggcac tctaacaact gaaaattcag
    22321 tattttcttc tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg
    22381 atcgggagtg acgtaatttg aggaataatg atgaaaaaaa ttgccatcac ctgtgcatta
    22441 ctctcaagct tagtagcaag cagcgtttgg gctgatgccg caagcgatct taaaagccgc
    22501 ctggataaag tcagcaactt ccacgccagc ttcacacaaa aagtgactga cggtagcggc
    22561 gcggcggtgc aggaaggcca gggcgatctg tgggtgaaac gtccaaattt attcaactgg
    22621 catatgactc aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat
    22681 aacccgttcg ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg
    22741 tttatgctga ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat
    22801 ggcgatgact ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt
    22861 aacgtgggac gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc
    22921 agcagttatc agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc
    22981 accccgccgc aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa
    23041 tctgtcgctc gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga
    23101 aaatttagca cagtatatcg gccagcaaca tttgctggct gcggggaagc cgttgccgcg
    23161 cgctatcgaa gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa
    23221 aacaaccctc gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc
    23281 tgtcacctct ggcgtgaaag agattcgcga ggcgattgag cgcgctcggc aaaaccgcaa
    23341 tgcaggtcgc cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca
    23401 ggatgcattt ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga
    23461 aaacccgtcg tttgaactta actcggcact gctttcccgt gcccgtgtct atctgctgaa
    23521 atccctgagt acagaggata ttgagcaggt gcttactcag gcgatggaag acaaaacccg
    23581 tggctatggt ggtcaggata ttgttctgcc agatgaaaca cgacgtgcca ttgctgaact
    23641 ggtgaatggc gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga
    23701 agtcgatgat agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga
    23761 acgtagcgcc cgctttgata acaaaggaga tcgcttttac gatctgattt ccgcactgca
    23821 taagtcggta cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac
    23881 cgctggtggc gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt
    23941 cggtaatgcc gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg
    24001 cgttggcccg gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcttgcgc
    24061 gccaaaaagc aacgctgtct acactgcgtt taaagccgcg ctggcggatg cacgcgaacg
    24121 tccggattat gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat
    24181 gggctacggg caggaatatc gttacgctca tgatgaagca aacgcttatg ctgccggtga
    24241 ggtttatttc ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct
    24301 tgaaggcaag attggcgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat
    24361 aaaacgctac cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca
    24421 ggctgtggcc acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa
    24481 tctgctgcgt aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct
    24541 ggatgtagat aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga
    24601 aaacctgcaa gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg
    24661 ggaagatatc gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc
    24721 aaaagccgag ctggatgctt tacaggctga aattcgcgat atcgcgctaa ccatccctaa
    24781 cctgcctgca gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg
    24841 ctggggcacc ccgcgtgagt ttgactttga agttcgtgac catgtgacgc tgggtgaaat
    24901 gcactctggc ctcgactttg cagccgcagt taagttgacc ggttcccgct ttgtggtaat
    24961 gaaagggcag attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac
    25021 cgaacagcat ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta
    25081 cggtacgggt cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga
    25141 agaagcagac accagtaact atgcgctgat cccaacggca gaagttccgc tgaccaacct
    25201 ggtgcgcggt gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc
    25261 atgcttccgt tctgaagccg gctcatatgg tcgtgacacc cgtggtctga tccgtatgca
    25321 ccagttcgac aaagttgaaa tggtgcagat cgtgcgcccg gaagactcaa tggcggcgct
    25381 ggaagagatg actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa
    25441 aatcatcctt tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt
    25501 atggatcccg gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt
    25561 ccaggcacgt cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt
    25621 tcataccctg aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa
    25681 ctatcagcag gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg
    25741 actggaatat attggctaat acccaatttt tctgaatcta aaaagcgcct gcgggcgctt
    25801 tttttgtctc cctttgatac cgaacaataa ttactcctca cttacacgta atactacttt
    25861 cgagtgaaaa tctacctatc tctttgattt tcaaattatt cgatgtatac aagcctatat
    25921 agcgaactgc tatagaaata attacacaat acggtttgtt actggaatca atcgtgagca
    25981 agcttgagtg agccattatg aaaacgaaaa tccctgatgc ggtattggct gctgaggtga
    26041 gtcgccgtgg tttggtaaaa acgacagcga tcggcggcct ggcaatggca agcagcgcgt
    26101 taacattacc ttttagtcgg attgcgcacg ctgttgatag tgcccttccg gcaaaatcag
    26161 acgaaaaggt tatctggagc gcctgtacag ttaactgtgg tagtcgctgc ccgctacgta
    26221 tgcacgttgt ggacggtgaa attaaatatg ttgaaacgga taataccggc gatgacaact
    26281 atgacggcct gcaccaggtg cgcgcctgtc tgcgtggtcg ttccatgcgt cgccgtgtct
    26341 acaatccgga ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat
    26401 tcgagcgcat tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga
    26461 tcaaagagta cggcaacgag tctatctatc tgaactatgg taccggaacg ctgggcggca
    26521 ccatgacccg ctcctggccg ccgggaaata ccctggtcgc tcgcctgatg aactgctgcg
    26581 gcggttatct gaaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaact
    26641 atacctacgg cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaactgg
    26701 tagtgctgtt tggtaataac cctggcgaaa cgcgaatgag tggcggtggg gtgacttact
    26761 atcttgaaca ggcgcgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata
    26821 ccgacaccgg tgccgggcgc gaagatgagt ggatccctat tcgtccggga acagatgccg
    26881 cactggttaa cggtctggcg tatgtcatga tcaccgaaaa cctggtggat caggcattcc
    26941 tcgataaata ttgcgttggc tacgacgaga aaacactgcc agccagtgcg ccgaaaaatg
    27001 gtcactataa agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acgccggaat
    27061 gggcctcgca aatcactggt gttccggcag acaaaatcat caaactggct cgtgaaatcg
    27121 gtagtaccaa accggcgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg
    27181 aaatcgcaac ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaacg
    27241 gaggcaacag cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgaccc
    27301 tcgaaaaccc gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg
    27361 gcccggaaat gacagcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga
    27421 tcaaaatgat ctggaactat gccggtaact gcctgattaa tcagcattct gaaatcaacc
    27481 gtacccatga aatccttcag gatgataaga agtgcgagct gattgtggtt atcgactgcc
    27541 acatgacctc atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttcagaac
    27601 agatggactt tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc
    27661 aggtgattaa accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa
    27721 aacgtcttgg cgttgagcaa cagtttactg agggccgtac ccaggaagag tggatgcggc
    27781 atctgtatgc ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgta
    27841 agcaggggat ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagctttcc
    27901 gtgaagatcc gcaggcaaat ccactgacca cgccatcggg gaaaattgag atttattcgc
    27961 aggcgctggc tgacattgcc gctacctggg aattgccaga aggtgatgtg atcgatccac
    28021 tgccgatcta cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc
    28081 tgcagcttac gggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg
    28141 tgctgaaagc agcttgccgt caggaaatgt ggatcaaccc gcttgatgcg caaaaacgcg
    28201 gtatcaacaa cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg
    28261 cgaaagtgac gccacgaatg atgccgggtg tggtcgcact gggtgaaggt gcctggtatg
    28321 acccggatac aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc
    28381 cgtctcctct cgctaagggg aatccgtcac atacaaacct ggttcaggtt gaaaaggtgt
    28441 aaggagtaac cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt
    28501 tgcaaaacct gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc
    28561 cgccgcattt atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag
    28621 aacgtgtttg cctactatct gtcgatttca tgtaaccact gcgaagatcc ggcttgtact
    28681 aaagtctgcc cgagcggtgc gatgcataaa cgtgaagatg gttttgttgt ggtcgatgaa
    28741 gatgtgtgca ttggctgccg ttactgccat atggcttgcc cgtacggcgc accgcaatat
    28801 aacgaaacga aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggg
    28861 aaaaagccga tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac
    28921 gagctgcgta aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt
    28981 accaaaccga atattgtgat caaacccaat gccaacagcc gcccgaccgg ggataccact
    29041 ggctatctgg caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct
    29101 gatgatcttc acggtcttcg ggcaatgtgt agcgggtggt tttatcgttc tggctttggc
    29161 gctgctcaaa ggcgacctgc gagcagaagc ccagcagcgt gttattgcct gcatgtttgg
    29221 tttatgggtg ctgatgggca ttggctttat cgcctctatg cttcatcttg gttcaccgat
    29281 gcgcgccttt aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag
    29341 cggttcgatc ttttttgctg ttggtggcat cggctggttg ctggcaatgc tgaaaaagct
    29401 gtcaccggca ttgcgtacgc tgtggctgat agtgacgatg gttcttggcg tcatctttgt
    29461 ctggatgatg gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg
    29521 gacgccgatg ggcttcttcc tgacgatgtt catgggcggc ccgttactgg gttacctgtt
    29581 gctgagtctg gctggcgtcg atggctgggc gatgcgtctg ctgccagcca tttctgtact
    29641 ggcactggta gtgagtggcg tgatgtcagt gatgcagggc gcagagctgg cgactattca
    29701 tagctctgtg cagcaggccg cagcgctggt gccggactat ggcgcgctga tgtcctggcg
    29761 gatcgtgctt ttggctgttg ccctgtgctt gtggattgca ccacagctaa aaggttatca
    29821 gcctgcggtt ccgctacttt ctgtatcatt cattctgctt ctggcagggg aattaatcgg
    29881 tcgtggcgta ttctatggtt tgcatatgac cgtggggatg gctgtcgcaa gttaatcagt
    29941 aacaaccggg gtttcggccc cggttcgctt ttatttctgc ttcgttaacg tgtcataact
    30001 ggtcatcaag ttacgataat caggaatgtg gttagagaac aacgtcgcca gtccttcaat
    30061 atcattacgc cagtcgcgat gcagctcgca tgccacgccg aaccatgtca tcaattgcgc
    30121 accagcttgc gacatgcgat cccatgccga atgccgggta atttcattaa aggtccctga
    30181 cgcgtcagtc acgacaaaga cgtcaaaccc ttcttcaatg gctgaaagcg ccgggaatgc
    30241 cacgcaaact tcggttacca caccggcaat aattaactgt tttttacctg tcgctttgac
    30301 agcttttaca aaatcttcgt tatcccaggc gttaatattt cccgggcgag caatgtaagg
    30361 tgcatcgggg aattgtgctt tcagttctgg aactaatggg ccgttaggac cggtttcaaa
    30421 actggtggta agaatagttg gtaagttgaa atacttagcc aggtcaccca gcgccagcac
    30481 gttattttta aacttatcgg gttcgatatc ccgtacaagg gaaagtaaac cagcctggtg
    30541 atcaacaagc aaaacggcag catcattttt atcaagacga acatacggtt tggtcatcct
    30601 cttctccttt cgagagtcag tggtacgtct gaaatcggtc agacgcgatg cattgctctg
    30661 aaagcataga cgggaaatat gagtttgctg tgaccatgaa atttttcgac tgaacgaagc
    30721 gtatgaaatt tgtgttagat caataaaaac aatcagatga gattaatcgt ctctccagca
    30781 ttagcgttat gaatgagtaa tataaatcga taatacctcc gctgcgatat gcgcgtattt
    30841 tcacccattg acaatgtttt tggcggtggc atgatgcgca tgaaatttga acttcctcac
    30901 ggttttaatt catgtccacg tatacccggc ctgtcatgct tttgctgtct ggcctgcttt
    30961 tgttgactct ggcgatcgca gtgttaaata cactcgtgcc gctttggctc gcccaggaac
    31021 acatgtccac atggcaggta ggcgttgtca gctcatccta ttttaccggc aacctggtcg
    31081 gtacattgct gacagggtat gtcattaagc gcattggctt taaccgcagc tattatctgg
    31141 cctccttcat ttttgccgct ggctgtgccg gccttggcct gatgattgga ttctggagct
    31201 ggttggcttg gcgttttgtc gccggtgtcg gttgtgccat gatttgggtg gttgttgaga
    31261 gcgcgctgat gtgcagtggg acgtcacgta accgtgggcg tttgcttgct gcgtatatga
    31321 tggtttatta cgtgggaacg tttttaggcc agttactggt cagcaaagtt tcaaccgagc
    31381 tgatgtccgt attgccgtgg gttacaggtt tgacgttggc agggatctta ccgctgttgt
    31441 ttacgcgtgt gctgaatcag caggctgaaa accatgattc gacgtcaatt acgtcaatgc
    31501 taaaactccg tcaggcgcgg cttggcgtga atggctgcat tatctcagga atcgtgctgg
    31561 gatctctata tggcctgatg ccgctgtacc tcaatcacaa aggggtgagc aatgccagca
    31621 ttggtttctg gatggcggta ctggtcagtg cgggtatcct cggacaatgg ccgatcggac
    31681 gtctggcgga taagtttggt cgactgctgg tgttgcgtgt tcaggtcttt gtcgtcattc
    31741 tcggcagtat cgcgatgctt agccaggcgg cgatggcccc agcgttattc atcctcggtg
    31801 ccgctggctt tacgctatat ccggtggcga tggcctgggc ttgtgagaaa gttgaacatc
    31861 atcaactggt ggcgatgaac caggccttac tgttgagcta taccgtggga agtctgcttg
    31921 gcccgtcatt taccgctatg ctaatgcaga atttctccga taatttattg tttatcatga
    31981 tcgccagcgt atcgtttatc tatttgctga tgctgcttcg caacgccggt catacgccga
    32041 aacccgttgc tcacgtgtaa atgaattcaa gcagagtgtg aacttactgt ttcacactct
    32101 gcttttttgt ttcttctatc tgacttgctt tattccaaat tttattcgtt taaaaataaa
    32161 atgtgcagca ggttataatt ttgcatttcg ctatttccgc acttcttatt tgccgcgcat
    32221 aatccctcgt tttacagatg cccctttaat tttggcgaag gatttgtcta tggctgggaa
    32281 tgttcaggaa aaacagctgc gatggtacaa cattgcgctg atgtctttta tcactgtctg
    32341 gggttttggc aatgttgtta ataactacgc caaccagggg ctggtggttg ttttttcatg
    32401 ggtgtttatc tttgcgctct atttcacacc ttatgcgcta attgttggtc agttaggctc
    32461 gaccttcaaa gatgggaagg gcggggtcag tacctggatt aaacacacga tgggacccgg
    32521 actggcttac ctcgccgcgt ggacctactg ggtagtgcat attccctatc tggcacaaaa
    32581 accccaggca atactgattg cgctcggttg ggcgatgaaa ggcgacggtt cgttaatcaa
    32641 agaatattca gtcgtagcgt tacaggggtt aacgctggtg ctgtttatct tctttatgtg
    32701 ggttgcttca cgcggtatga aatcgctgaa aatcgtcggt tctgtggcag ggattgcgat
    32761 gtttgttatg tcgctcctgt atgtggcgat ggcggtaacc gcgcctgcaa ttactgaagt
    32821 gcatattgcg accacaaaca ttacctggga aacgttcatt cctcatatcg actttaccta
    32881 cattaccact atttcaatgc tggttttcgc ggttggcgga gcagagaaga tttctcctta
    32941 cgttaatcaa acgcgcaacc caggaaaaga atttccaaaa gggatgttat gcctggcggt
    33001 gatggttgcg gtttgtgcca ttctgggttc actggcgatg gggatgatgt ttgactcgcg
    33061 taatatcccg gatgacttaa tgactaacgg tcagtattac gcctttcaga agctgggcga
    33121 gtattacaac atgggtaata ctttaatggt gatttacgcc attgcgaata ccctgggaca
    33181 agtagctgcg ctggtattct cgattgatgc cccgcttaaa gtgctattag gcgatgctga
    33241 cagcaaatat attccagcca gtttatgtcg taccaacgct tctggtacgc ccgttaatgg
    33301 ctattttctg accctggtgc tggtggcgat cctgataatg ctaccgactc tcggcattgg
    33361 tgatatgaac aatctctata aatggctgtt gaatcttaac tcggtagtga tgccgctgcg
    33421 ttatttatgg gtatttgttg catttattgc agttgttcgc ctggcgcaga aatataagcc
    33481 ggaatatgtc tttattcgta ataagccgct ggcaatgacc gtcggaattt ggtgttttgc
    33541 ctttaccgct tttgcctgcc tgactggtat tttcccgaaa atggaagcct tcaccgcaga
    33601 gtggacattc cagttggcgc tgaatgttgc aacgccgttt gtgctggtag gattagggct
    33661 gatattcccg ctgctggcgc gtaaagcgaa tagcaaataa ttattgtggt cggtgatgct
    33721 gactctggca gatctgaccc gtttttacat aatcgagaaa tgcgcgcaag ccgcaggaca
    33781 tatactggcg gtttgggtaa tacatttgga agctgtgctg taagggcgtt agaaatagag
    33841 ctctgaatta tttatgcagc gcggaataaa aaagcggcaa ctcaaaaaag ttgccgcttt
    33901 acggggaaat tagaacatta ccttatgacc gtactgctca agaatgcctt tcacgcgttc
    33961 catggtctct ttcttcggtg gtttaacacc atcaagtttg tattcttcac ccatcgccac
    34021 ccatttgtgt ttgcccagtt cgtggtaggg gagaagctcg attttctcaa cgttgcccat
    34081 atcacgggta aattcaccga ggcgatgcgc tgaatcgtca tcgtcagacc agcctgggac
    34141 aacaacgtag cggatccaca ccttcacatt tttgttcgcc agatatttgg cgaactccag
    34201 cgtgcggtgg ttggaaactc caaccagatt ttggtggatc tcgtcgttca tctgtttgag
    34261 atcgagcatt accaggtcgg ttacttccag cagttcatca atcaccggat cgtaacgacg
    34321 aacaaaaccg ttggtgtcca gacaggtatg aatgccttct tttttgcagg cgcggaacca
    34381 gtcacgaaca aactcagctt gcaggattgc ttcaccgccg gatgcggtaa cgccgccgcc
    34441 ggaagcgttc ataaagtggc gataggtcac cacctctctc attaaatctt caacggtaac
    34501 ttctttaccg ccatgcgtgt cccaggtgtc gcggttatga caatacaggc agcgcatcag
    34561 gcagccctgg aaaaaggtga taaagcgaat acccggaccg tctacggttc cacaggattc
    34621 aaaggagtga atgcgaccaa taactgacat tgcggtgttt ctccagatgt ggcccatctg
    34681 aggccgtgtt ggtgcgcagc tcgaaggcta cgtcgagtct gttttggcag tcaccttaaa
    34741 gtatagatag ctgacaaaaa aggctctcgt gctaaaaaag gccccacttt cgtggagcct
    34801 ttattgtacg ctttttactg tacgatttca gtcaaatcta attacataga ttgagtgaag
    34861 gtacgagtaa taacgtcctg ctgctgttct ttagtcagcg agttgaaacg tactgcgtag
    34921 ccagatacac ggatggtcag ctgcggatat ttttccgggt tttccatcgc gtcgagcagc
    34981 atttcacggt tcatcacgtt aacgttcagg tgctgaccac cttcgatgga cgcttcgtgg
    35041 tggaagtaac catccatcag accagccagg ttggtcttac gaacttcgtc gtctttaccc
    35101 agtgcgttcg gaacgataga gaaggtgtag gagataccat ctttagcgta agcaaacggc
    35161 agtttagcaa cggaagtcag agaggctact gcacctttct ggtcacgacc gtgcatcggg
    35221 ttagcacccg gtccgaacgg cgcgccagca cgacgaccgt ctggggtgtt accagttttc
    35281 ttaccataca caacgttaga agtgatggtc agaacagact gagtcgggat agcgtcacgg
    35341 taggtgtgca gtttctgaat tttcttcatg aaacgttcta ccaggtcaac agccaggtca
    35401 tctacacgcg gatcgttgtt accaaactgc gggtattcgc cttcgatttc gaagtcgata
    35461 gccagaccgt cttcgtcacg aatcggttta actttcgcat atttgattgc agacagggag
    35521 tcagcagcaa cggacagacc agcgatacca cacgccatgg tgcggataac gtcacggtcg
    35581 tgcagcgcca tcagagaggc ttcgtagctg tacttgtcgt gcatgtagtg gatgatgttc
    35641 agtgcagtga tgtactgttt agccagccag tccatgaagt gatccatgcg ctccatcact
    35701 tcatcgtagt tcaggagatc gcctttgatc ggctcagact tcggaccaac ctgcattttc
    35761 agtttttcgt caacgccgcc gttgattgcg tacagcatgg ttttcgccag gtttgcacgc
    35821 gcaccgaaga actgcatttg tttaccaacg atcatcgggc ttacgcagca agcgatagcg
    35881 tagtcatcgt tgttgaagtc cggacgcatc aggtcatcgt tctcatactg cagagaagag
    35941 gtgtcgatgg acactttagc agcgaatttc ttgaagttca gcggcagttt ttcggaccac
    36001 agaatggtca tgttcggttc cggagacgga cccatggtgt acagggtgtt caggaaacgg
    36061 aagctgtttt tggtaaccag ggtacgaccg tcgaggccca taccaccgat agattcggtt
    36121 gcccagatcg ggtcgccaga gaacagttca tcgtattccg gagtacgcag gaagcgaacc
    36181 atacgcagtt tcatgaccag gtggtcaacc atttcctgcg cttcttgttc ggtgatcttg
    36241 ccagctttca ggtcacgttc gatgtacaca tccaggaagg tggaggtacg accgaaggac
    36301 attgcagcac cgttctgaga cttaacagca gccaggtagc cgaagtaagt ccactggata
    36361 gcttcctgag cgttggtagc cggaccagag atgtcgtagc cgtatttcgc agccatttct
    36421 ttcatctgac ccagagcgcg gtgctgttca gcgatttctt cgcgcagacg gatagtctgt
    36481 tccaggttta cgccgttttc cagatcagcc tgcagagagg tgaactgagc gtatttgtct
    36541 ttcatcagat agtcgatacc gtacagcgca acgcgacggt agtcaccgat gatacgacca
    36601 cggccataag catctggcag accggtcaga acaccggatt tacggcaacg caggatgtcc
    36661 ggagtgtaaa cgtcgaacac gccctggttg tgagttttac ggtattcagt gaagattttt
    36721 ttgatcatcg ggtccagttc gcggttgtac gctttgcagg aaccttcgat cattttgata
    36781 ccaccgaacg ggataagagc acgtttcagc ggagcttcag tctgcagacc aacaactttt
    36841 tccaacgctt tgttgatgta gccagcgtcg tgagaggtga tggtggaagc aacagcggtg
    36901 tcaaagtcaa ctggcgcgtg agtgcggttt tccagtttaa cgccttccat tactttgtcc
    36961 cacagggtgg tggtcgcttc agtagcgcca gccaggaagg actcgtcacc ctcgtacgga
    37021 gtgtagtttt tctgaatgaa gtcacggacg tttacttcat tctgccagtc acctttggta
    37081 aaaccttccc aggctgtggc taacttttca ttaagctcgg acatgtaaca cctaccttct
    37141 taagtggatt ttttatttac tgcgtacttc gacaaccatt aatggtgatc gttttcacgc
    37201 agataaatga cccagtatgt caacccaacc aacaaaccac cgccgataat attaccgatc
    37261 gtaaccggaa tcaggttatc agtgatgaag ttcatcacgg tcaggtgaga aaaattttcc
    37321 ggtgcagaac cgacagcggt ccagaattcc ggagatgcga agtcgcggat tacaataccc
    37381 atcgggatca taaacatgtt tgcgatactg tgctcaaaac cgctggcaac aaacatcgcg
    37441 accggcagca ccataatgaa cgctttatcc atcaggctgc ggccagaata actcatccat
    37501 actgccagac ataccatcag gtttgccagg ataccaagac agacggcctc aataaaagta
    37561 tggtgcactt tgtggtcggc ggtttgtagg acgtttagtc cccattgacc attcgcggtc
    37621 atatactcgc cggaaagcca cattaaaagt acaaacagca gtgcgccgac caggttgcca
    37681 aaatagacat ttagccagtt tttcgctaac tgaccccagg tgatgcgccc actcgcctta
    37741 gcaacaacaa tcaacactgt ggaagtaaag agatcggctc cgcagacaac acaaagaatc
    37801 agccccagag agaagcaaat gccgccaacc agttttgcca tgccgaaggg cattgttcct
    37861 gtgccagtgg ttgctgtgat atagaagacg aatgcgattg agatgaaaac accggcggta
    37921 atcgccagat agaaagtctt aagcggatgt ttcgttgctt tatagacacc tgcctcttcg
    37981 gccactttgg ccattgctgc aggaagtaaa agatcaaaag ggttgtcagc tttcacacta
    38041 actctctctt tattaagtcg gcgacgagat actaacaaag cattatagat gagaaattga
    38101 tatagatcat atctcgcctg gcttataggc ccgtaactcg catggttttt atgcaaatac
    38161 ggagtaaata tttgattatc caaataa
//
LOCUS       DAZGHF010000029        37561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGHF010000029 DAZGHF010000000
VERSION     DAZGHF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.029"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            324..1697
                     /gene="aroP"
                     /locus_tag="ACHK3A_001632"
     CDS             324..1697
                     /gene="aroP"
                     /locus_tag="ACHK3A_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO8739724.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTCFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1740..2594)
                     /gene="ampE"
                     /locus_tag="ACHK3A_001633"
     CDS             complement(1740..2594)
                     /gene="ampE"
                     /locus_tag="ACHK3A_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO8739725.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQIDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVLVVIAL
                     LTIYGALV"
     gene            complement(2591..3142)
                     /gene="ampD"
                     /locus_tag="ACHK3A_001634"
     CDS             complement(2591..3142)
                     /gene="ampD"
                     /locus_tag="ACHK3A_001634"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO8739726.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLVAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3230..4123
                     /gene="nadC"
                     /locus_tag="ACHK3A_001635"
     CDS             3230..4123
                     /gene="nadC"
                     /locus_tag="ACHK3A_001635"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO8739727.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANE
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4326..4766
                     /gene="ppdD"
                     /locus_tag="ACHK3A_001636"
     CDS             4326..4766
                     /gene="ppdD"
                     /locus_tag="ACHK3A_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO8739728.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4776..6161
                     /gene="gspE"
                     /locus_tag="ACHK3A_001637"
     CDS             4776..6161
                     /gene="gspE"
                     /locus_tag="ACHK3A_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO8739729.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAISFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVENPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAINAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6151..7353
                     /gene="hofC"
                     /locus_tag="ACHK3A_001638"
     CDS             6151..7353
                     /gene="hofC"
                     /locus_tag="ACHK3A_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO8739730.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALFPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPVLTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNAGEFSPLCLQLVRTE
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7388..8431)
                     /gene="guaC"
                     /locus_tag="ACHK3A_001639"
     CDS             complement(7388..8431)
                     /gene="guaC"
                     /locus_tag="ACHK3A_001639"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO8739731.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSFEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(8587..8631)
                     /locus_tag="ACHK3A_001640"
     CDS             complement(8587..8631)
                     /locus_tag="ACHK3A_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739732.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8656..9276
                     /gene="coaE"
                     /locus_tag="ACHK3A_001641"
     CDS             8656..9276
                     /gene="coaE"
                     /locus_tag="ACHK3A_001641"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO8739733.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     PREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9276..10019
                     /gene="zapD"
                     /locus_tag="ACHK3A_001642"
     CDS             9276..10019
                     /gene="zapD"
                     /locus_tag="ACHK3A_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO8739734.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPVVDYAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            10029..10226
                     /gene="yacG"
                     /locus_tag="ACHK3A_001643"
     CDS             10029..10226
                     /gene="yacG"
                     /locus_tag="ACHK3A_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO8739735.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10428..10826)
                     /gene="mutT"
                     /locus_tag="ACHK3A_001644"
     CDS             complement(10428..10826)
                     /gene="mutT"
                     /locus_tag="ACHK3A_001644"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO8739736.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHLSLFEILEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVLAKLKRVYVG"
     gene            complement(10886..13591)
                     /gene="secA"
                     /locus_tag="ACHK3A_001645"
     CDS             complement(10886..13591)
                     /gene="secA"
                     /locus_tag="ACHK3A_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO8739737.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13653..14165)
                     /gene="secM"
                     /locus_tag="ACHK3A_001646"
     CDS             complement(13653..14165)
                     /gene="secM"
                     /locus_tag="ACHK3A_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO8739738.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14396..15313)
                     /gene="lpxC"
                     /locus_tag="ACHK3A_001647"
     CDS             complement(14396..15313)
                     /gene="lpxC"
                     /locus_tag="ACHK3A_001647"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO8739739.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15414..16565)
                     /gene="ftsZ"
                     /locus_tag="ACHK3A_001648"
     CDS             complement(15414..16565)
                     /gene="ftsZ"
                     /locus_tag="ACHK3A_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO8739740.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16626..17888)
                     /gene="ftsA"
                     /locus_tag="ACHK3A_001649"
     CDS             complement(16626..17888)
                     /gene="ftsA"
                     /locus_tag="ACHK3A_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO8739741.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17885..18715)
                     /gene="ftsQ"
                     /locus_tag="ACHK3A_001650"
     CDS             complement(17885..18715)
                     /gene="ftsQ"
                     /locus_tag="ACHK3A_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO8739742.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18717..19637)
                     /gene="ddlB"
                     /locus_tag="ACHK3A_001651"
     CDS             complement(18717..19637)
                     /gene="ddlB"
                     /locus_tag="ACHK3A_001651"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO8739743.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDANPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19630..21105)
                     /gene="murC"
                     /locus_tag="ACHK3A_001652"
     CDS             complement(19630..21105)
                     /gene="murC"
                     /locus_tag="ACHK3A_001652"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO8739744.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21159..22226)
                     /gene="murG"
                     /locus_tag="ACHK3A_001653"
     CDS             complement(21159..22226)
                     /gene="murG"
                     /locus_tag="ACHK3A_001653"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO8739745.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22223..23467)
                     /gene="ftsW"
                     /locus_tag="ACHK3A_001654"
     CDS             complement(22223..23467)
                     /gene="ftsW"
                     /locus_tag="ACHK3A_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO8739746.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23467..24783)
                     /gene="murD"
                     /locus_tag="ACHK3A_001655"
     CDS             complement(23467..24783)
                     /gene="murD"
                     /locus_tag="ACHK3A_001655"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO8739747.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAVTGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24786..25868)
                     /gene="mraY"
                     /locus_tag="ACHK3A_001656"
     CDS             complement(24786..25868)
                     /gene="mraY"
                     /locus_tag="ACHK3A_001656"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO8739748.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25862..27220)
                     /gene="murF"
                     /locus_tag="ACHK3A_001657"
     CDS             complement(25862..27220)
                     /gene="murF"
                     /locus_tag="ACHK3A_001657"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO8739749.1"
                     /translation="MISVTLSQLTDILNGELKGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVNRPLDIDLPQVIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHIQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27217..28704)
                     /gene="murE"
                     /locus_tag="ACHK3A_001658"
     CDS             complement(27217..28704)
                     /gene="murE"
                     /locus_tag="ACHK3A_001658"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO8739750.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28691..30457)
                     /gene="ftsI"
                     /locus_tag="ACHK3A_001659"
     CDS             complement(28691..30457)
                     /gene="ftsI"
                     /locus_tag="ACHK3A_001659"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO8739751.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30473..30838)
                     /gene="ftsL"
                     /locus_tag="ACHK3A_001660"
     CDS             complement(30473..30838)
                     /gene="ftsL"
                     /locus_tag="ACHK3A_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO8739752.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30835..31776)
                     /gene="rsmH"
                     /locus_tag="ACHK3A_001661"
     CDS             complement(30835..31776)
                     /gene="rsmH"
                     /locus_tag="ACHK3A_001661"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO8739753.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31778..32236)
                     /gene="mraZ"
                     /locus_tag="ACHK3A_001662"
     CDS             complement(31778..32236)
                     /gene="mraZ"
                     /locus_tag="ACHK3A_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO8739754.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32838..33842)
                     /gene="cra"
                     /locus_tag="ACHK3A_001663"
     CDS             complement(32838..33842)
                     /gene="cra"
                     /locus_tag="ACHK3A_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO8739755.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33924..34010)
                     /locus_tag="ACHK3A_001664"
     CDS             complement(33924..34010)
                     /locus_tag="ACHK3A_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO8739756.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(34022..34513)
                     /gene="ilvN"
                     /locus_tag="ACHK3A_001665"
     CDS             complement(34022..34513)
                     /gene="ilvN"
                     /locus_tag="ACHK3A_001665"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO8739757.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34516..36240)
                     /gene="ilvI"
                     /locus_tag="ACHK3A_001666"
     CDS             complement(34516..36240)
                     /gene="ilvI"
                     /locus_tag="ACHK3A_001666"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO8739758.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36359..36403
                     /locus_tag="ACHK3A_001667"
     CDS             36359..36403
                     /locus_tag="ACHK3A_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739759.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(36558..37502)
                     /gene="leuO"
                     /locus_tag="ACHK3A_001668"
     CDS             complement(36558..37502)
                     /gene="leuO"
                     /locus_tag="ACHK3A_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO8739760.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9059 a        10463 c         9168 g         8871 t
ORIGIN      
        1 tattgtgctg gtggctaagg catagttttc agcaggcaga caaatccggc atatagctag
       61 ttaaaacagt atgttatcag acttaaagta tctattaaat gtgatgaaaa acttacaatt
      121 gctttttttt tatccaaaaa atcaacgcat ctacataaag caggtgcatt cccgggcaca
      181 taccattatc ttgtgatagt taaaagtagc attatcataa ttcagttaca tgaaacgtaa
      241 agaaatcaat acaacaaacg gaattgcaaa cttacacacg catcactgcg tagatcaaaa
      301 aaacaatcac cgcacgaggt ttcatgatgg aaggtcaaca gcacggcgag cagctaaagc
      361 gcggtcttaa aaaccgccat attcagctta tcgcgctggg tggcgcgata gggaccgggc
      421 tattcctggg tagcgcctcc gtaatacagt ccgcagggcc agggattatc ctgggttacg
      481 ccattgctgg ttttatcgcc tttctgatca tgcgtcagct aggtgaaatg gtggtcgaag
      541 aacctgtcgc aggctccttt agccactttg cttataaata ctggggcagt tttgccggtt
      601 tcgcctctgg ctggaactac tgggtactgt acgttttagt tgccatggct gagctgactg
      661 ccgtgggtaa atacattcag ttctggtatc cggaaatccc aacctgggtt tctgccgccg
      721 tgttctttgt ggtgattaac gccatcaacc tgaccaacgt taaagtgttt ggcgagatgg
      781 agttctggtt tgccattatc aaagttatcg cggtggtagc gatgatcatc ttcggcggct
      841 ggctgctatt cagcggcaac ggcggcccgc aggcgagcgt tagcaacctg tgggatcagg
      901 gtggtttcct gccgcacggc ttcaccgggc tggtgatgat gatggcgatt atcatgttct
      961 ctttcggcgg tctggaactg gtggggatca ccgcagcaga agctgataat ccggagcaaa
     1021 gtatcccgaa agcaactaac caggttatct accgcatcct gattttctat attggttcgt
     1081 tagccgttct gctctcgctg atgccatgga cccgcgttac tgctgatacc agtccgtttg
     1141 tactgatctt ccacgaattg ggcgatacct ttgtggctaa tgcgctgaac atcgtggtac
     1201 tgactgctgc gctctccgtg tacaacagct gcgtatattg caacagccgt atgctgtttg
     1261 gtctggcaca acagggcaac gcgccaaaag cgctggcgtc tgtcgacaaa cgcggtgtac
     1321 cggttaacac tattctggtg tctgcgctgg ttacggcatt gtgcgtgctg attaactacc
     1381 ttgcaccgga atccgcattc ggactgttaa tggcactggt ggtatctgca ctggtaatca
     1441 actgggcgat gattagcctg gcgcatatga aattccgtcg cgccaagcag gaacaaggcg
     1501 tggtaacttg cttccctgct ctgctttatc cgctgggtaa ctggatctgc ctgctgttta
     1561 tggcggcggt actggtgatt atgctgatga ccccaggaat ggcgatttcg gtatacctga
     1621 tcccggtatg gctgatcgtg ttaggtatcg gctatctgtt taaagagaaa accgccaaag
     1681 ccgtaaaagc gcattaatct ctctacgccc tcacccgtgc agggtgaggg caataatctt
     1741 tacaccaacg ccccgtaaat cgtcagtagc gcaattacca ccaggaccac gaacgaggtt
     1801 ttcttcgcca tggaaaccgc tgccttcggc gtctccacct tatcgacatg cggttcacgc
     1861 gccagagaga actgtgccag acgtgttaat acctgatatt gcgaagtatg gaaatcaccc
     1921 agcgaagcaa accaggccgg taacgctttc tccccatgac cgatcaaggc atataccaca
     1981 cccgcaagac gaaccggcac ccaatccagt acatgaagca cggcatcaat gccggactgt
     2041 aaacgatgat gcggcgtctg gtaacgcgcc agccagtatt gccatgcacg caaaaacgca
     2101 taccccatca gcgtaacggg tccccaggtt ccccccacaa tcagccagaa cagcggtgca
     2161 agataaaaac gaaagttaat ccacagcaat gcattttgca gctcacgcaa atactcacgt
     2221 tcatcgcagc ctgccgggac gccgtgaatc atggtgagtt cgccagccat cgtggcacgg
     2281 gcatggctat cattacgtga agcagctgtc agataagcat gataatgaag acgaacttta
     2341 cctgcgccaa tacacagcaa accaatcagc agccacacca gcagcgtggg aacgttgaac
     2401 aatactccct gcaatgcgcg taacagtaaa aaagtcacgc ccatcgcaat aatggtcatg
     2461 cctaatgtgc gcccgagaga aaaatgtttc acccgccgaa agaacgcttc aagacgatga
     2521 tcaatctgcc agtgctcgcc caacttaaac aggcgctcga aaattaacac cagtaaggtt
     2581 gtaaatagcg tcatgttgtc tccttgctga ccagtgcacg aaaccgtgcc caatcaaatg
     2641 cagggccggg atcggttttc cgatccggcg caatatcaca atggcccgtc atgttattag
     2701 cgatatctgg atagcgatca atcagtgccc gcgtaaccgc cacaagctgt tgatactgcg
     2761 catcggtata cgccagcgta tcggtgcctt ccagctcaat cccaatagaa aaatcattgc
     2821 agcgttcgcg cccctgatac tgagagactc cggcatgcca ggcacgttta tcgaaaggaa
     2881 catactggac tatttcacca tcgcggcgaa tcaaacagtg agcggagacg cgcaaatggg
     2941 cgatctcagc aaagaaagga tgcgcctgcg gatcaatagt tccagtgaat aatgcgtcga
     3001 tccacggacc gccaaactcg cctggcggca ggctaatatt gtgcaccacc agcagggtgg
     3061 gtgtttcgtc atccgggcgg caatcgtaat gtggtgaggg aacgcggcgc gcgccaacca
     3121 gccacccctg ttctaacaac atgcaggatc tccttatatg tggtgctaat acccggttca
     3181 gagtagcatg tttctacctt atgattcgtt agctatctgg agttttaaca tgccgcctcg
     3241 ccgctataac cctgacaccc gacgtgacga gctgctggaa cgcattaatc tcgatatccc
     3301 cggcgcggtg gcccaggcgc tgcgggaaga tttaggcgga acagtcgatg ccaacaatga
     3361 cattacggca aaacttttac cggaaaactc tcgctctcat gccacagtga tcacacgcga
     3421 gaatggcgta ttttgcggca aacgctgggt tgaagaggtg tttattcaac tggcaggcga
     3481 cgatgtcacc ataatctggc atgtggatga cggcgatgtc atcaacgcca atgaaccctt
     3541 gttcgaactt gaaggcccat cccgcgtgct gttaacgggc gaacgcactg cacttaattt
     3601 tgtgcaaacc ctttcagggg ttgccagtaa ggtacgccac tatgtcgaat tactggaggg
     3661 caccaacacg cagttgttgg atacgcgcaa aaccttaccc ggcctgcgtt cagctctgaa
     3721 atacgcggta ctttgcggcg gcggagcgaa tcaccgcctg gggctttctg atgccttcct
     3781 gatcaaagaa aaccatatta ttgcctccgg ctcagtgcgc caggcggtcg aaaaagcgtc
     3841 ctggctgcac ccagatgcgc cagtagaagt cgaagtagag aatctggaag aacttgatga
     3901 agccctgaaa gcaggtgccg atatcatcat gctggataac ttcgaaacag aacagatgcg
     3961 cgaagccgtc aaacgcacca acggcaaggc gctactggaa gtgtctggca acgtcactga
     4021 caaaacactg cgcgaatttg ccgaaacggg cgtggacttt atctccgtcg gtgcgctaac
     4081 taaacacgtg caggcactcg acctttcaat gcgttttcgc tgattgctga cagatttatc
     4141 cgctccatcg cgggcggata atctcccctt tcccaacttt cttcgtaacg cctcgcaaat
     4201 ttcttgtctt tcgctgcaac gtaataaaaa actccggaag ctgctttccc gtttcgtttt
     4261 ttgcccctcg cttgtgctga tggcgtttgc caaagtagcg ccaaccaaat caaggagcga
     4321 aacaaatgga caagcaacgc ggttttacac ttatcgaact gatggtggtt attggcatca
     4381 ttgccatttt aagcgccatt ggtattcccg cttatcaaaa ctacctgcgc aaagccgcac
     4441 tcaccgacat gctacaaacc tttgtgcctt accgtaccgc cgtagagttg tgcgcgctgg
     4501 aacatggtgg attagatacc tgcgacggtg gcagcaatgg cattccctcg cctaccacca
     4561 cccgctatgt ttcagccatg agtgtggcaa agggcgtggt gtcgctgacc gggcaagaaa
     4621 gtctcaatgg gctaagcgtc gtcatgacac cgggttggga taacgcaaac ggcgtcaccg
     4681 gctggacgcg caactgcaat attcaaagtg acagcgcatt gcagcaagcc tgcgaagatg
     4741 tctttcgctt tgatgacgcc aactaaggag cggcaatgaa tattccacag ctcaccgccc
     4801 tgtgtctgcg ttatcaggga gtcttgctgg atgccagcga agaggtggtt catgttgcgg
     4861 tggtcgatgc cccttcgcat gagctactgg acgcattgca tttcgctacc accaaacgta
     4921 ttgagatcac ctgctggacg cgccaacaaa tggaaggtca cgccagtcgc acccaacaga
     4981 cattgcccgt agctattcat gagaagcatc agcccaaagc agagttgctg actcgaacgt
     5041 tacaatctgc gctggaacaa cgcgcgtctg atattcatat cgaaccagcg gacaatgcct
     5101 accgcatccg cttgcgtatc gacggtgtat tgcatccttt accggatgtt ttaccggatg
     5161 ccggagtcgc attaaccgcc agattaaaag tgctgggcaa cctggatatt gcggaacatc
     5221 gcctgccgca ggacgggcaa ttcactgtcg aactggcagg aaacgccatc tcatttcgta
     5281 ttgcgacctt accatgtcgg ggtggtgaaa aggtggtatt aaggttgtta cagcaggtga
     5341 gtcaggcact ggatgtcaac acgcttggaa tgcagccgtt acaactggcg gactttgctc
     5401 atgccttgca acaaccacag ggactggtgc tggtaacagg ccctacaggc agcggcaaaa
     5461 cggtcacgct ttatagtgcc ctgcaaacgc tgaataccgc tgacattaat atttgtagcg
     5521 tcgaaaatcc ggttgagatc cccatagccg gactaaacca gacgcaaatc catccgcgtg
     5581 ccggactcac ctttcagggc gttttacgtg cgttattgcg ccaggatcct gacgtcatta
     5641 tgatcggaga gatccgcgat ggcgaaacgg cagaaattgc cattaacgcc gcacaaacgg
     5701 gtcatctggt attgtccacc ctacacacca actccacctg cgaaacgctg gtacgtttac
     5761 agcaaatggg agtcgcccgc tggatgctct catcagcgct tacgctggta atagcccagc
     5821 gtctggtacg caaactttgc ccacattgtc gccgacagca aggggagcca atccatattc
     5881 cagacaatgt atggccgtcg ccgctgcccc gctggcaagc acccggttgt gtacattgct
     5941 accacggttt ttatggtcgc acggccttat ttgaagttct gcccataaca ccggtcatac
     6001 gtcagcttat ttccgctaat accgacgttg aatcgctgga aacgcacgcc cgacaggcgg
     6061 gtatgcgaac gctttttgaa aacggctgtc tggccgtgga gcaaggctta accacctttg
     6121 aagagttaat ccgcgtattg gggatgccgc atggcgagta agcaactctg gcgctggcgt
     6181 ggcataaccg gcgacggcaa tgcgcaagat gggatgctat gggcagagag ccgtgctttg
     6241 ctgcttatgg cactacagca acagatggtt accccactaa gcctgaagcg aatcgccatc
     6301 aattctgcgc agtggcgagg agataaaagc gcggaagtca ttcatcaact ggcgacgcta
     6361 ctcaaagccg ggttaacgct ttctgaaggg ctggcactgc tggcggaaca gcatcccagt
     6421 aagcaatggc aagcgttgct gcaatcgctg gcgcacgatc ttgaacaggg cgttgccttt
     6481 tccaatgcct tatttccctg gtcagaggta tttccgccgc tctatcaggc gatgatccgc
     6541 acgggtgaac tgaccggtaa gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa
     6601 gcccagcgtc agttgaccga caaagtgaaa tcagcgttac gttatcccat tatcatttta
     6661 gcgatggcaa tcatggtggt tgtggcaatg ctgcattttg tgctgccgga gtttgctgct
     6721 atttataaga ccttcaacac cccactaccg gtactaacgc aggggatcat gacgctggca
     6781 gactttagtg gcgaatgggg ctggctgctg gtgttgttcg gatttctgct ggcgatagcc
     6841 aataagttgc tgatgcgccg cccgacctgg cttatcgcga ggcagaaatt gctgttacgc
     6901 atcccgatta tgggttcact gatgcgggga caaaaactca cgcagatctt tacgattctg
     6961 gcgctgactc aaagtgcagg cattactttt ttacagggcg tagagagcgt cagagaaaca
     7021 atgcgctgcc cgtactgggt gcaacttctg acacaaatcc agcacgatat cagtaacggt
     7081 caacccatct ggctggcgct aaaaaatgcc ggtgagttta gcccactctg tttgcaatta
     7141 gtgagaacag aagaggcatc cggctcgctg gatctcatgt tagacaacct cgcccatcat
     7201 catcgggata acacaatggc gctggcggat aacctcgcag ccttactgga accggcgttg
     7261 ctgatcataa cgggaggaat tatcggtacg ctggtggtgg caatgtatct gccaattttc
     7321 catttaggcg atgcgatgag tggaatggga taacgctggc gtgttgctcc acgccagcgt
     7381 tgggagatta caggttgttg aagatgcggt tttcttgttc cagcacacgg ataaatgtgg
     7441 tgcgcttggt cagctctttc aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc
     7501 cgcccaaaat atcacgcgcg gtattttcaa ccgggcctcg cagcggcagc ttaacggttt
     7561 taccttctgc tgcgcgatat tccgcaacgc caccaacgtg acgtttcatc gcagactcgg
     7621 agctcatgcc gtagaacagc ataaatttct cgccgttctc ctcaacgatg cgaccgccgc
     7681 tctcttcgtg gcccgccagc atgccgccaa gcatgacgaa atcggcaccg ccgccaaagg
     7741 ctttcgcgac atcgcccggc gtggtgcagc caccgtcgct gacgatcatt ccgcccagac
     7801 cgtgcgcagc atcggcacat tcgatcaccg cagaaagttg cggataaccg acgcctgttt
     7861 tgacgcgagt tgtacaaaca gaacctgggc caatgccaac tttaacgata tcagcaccgg
     7921 agaggataag ctcctcacac atttcaccag tcactacgtt gccagcacaa atggttttgg
     7981 tcggccacgc ttcacgcgct ttcgcaacga actgcacgaa gtgttcggaa taaccattcg
     8041 ccacgtcaat acaaacgaag tttaatgccg ggttcaggtc gagaatctgt ttagtttttt
     8101 cgaaatccgc atcagacgtg ccggtagaaa ccatcacatg tttcagcaca tcagcggaag
     8161 aattgttgat aaacgcttgc cactcttcga aagaatagtg tttatgcaca gcagtcaaaa
     8221 tatcaaaaga agccagcgca gaggccatag aaaatgtgcc tacggtgtcc atatttgcgg
     8281 cgataatcgg cacgccggac cagctctgac ctgaatgttt gaaggtgaat tgacgttcca
     8341 gttcaacatc ggaacggctt ttaagagtgg agcgtttagg gcggatgaga acgtctttaa
     8401 aacctaactt cagatcttct tcaatacgca tgtgcggatt cctggggtta atggctataa
     8461 gggtaagagc gcaactccag tgacgctatc atacgcacta atcaatgcgg cgcaagactg
     8521 cgaaattgtc tttttttacg ctacaatccc ataaatttac ctggtaaata gtgagcgaac
     8581 gtttcatcaa tttcgctttc gtgcgctcat ttttaacgtt ttgataatca tgatgaattc
     8641 ccgggaatat aaattatgag gtatatagtt gccttaacgg gaggcattgg cagtggcaag
     8701 agtaccgttg ccaatgcgtt tgctgatctc ggaattaacg tcattgatgc cgatattatt
     8761 gcgcgtcagg tggttgaacc aggtgcacct gcgctacatg ccattgctga tcactttggc
     8821 gctaacatga ttgctgctga tggaacattg cagcgccggg ccttgcgcga gcggatcttc
     8881 gccaacccgg aagagaaaaa ctggcttaac gccctgctgc atccgctgat tcagcaagag
     8941 acgcaacacc agatccagca agcaacctcc ccctatgtac tgtgggttgt gccattgctg
     9001 gtagaaaact cactgtataa aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa
     9061 acgcaactta agcgcaccat gcagcgcgat gatgtaactc gcgagcatgt cgaacaaatc
     9121 cttgctgctc aggcaccgcg cgaagctcgc cttgccgtgg cagatgacgt cattgataat
     9181 aacggcgcac cggatgctat cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag
     9241 cttgcgtcgc agtttgtctc acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac
     9301 atccactaaa tgaaaaaatg cgtacatggc tgcgcattga gtttttaatt caacaactca
     9361 ccgtaaattt gcccgtcgtt gactacgctg gcgctctgca tttcttccgc aatgtcagtg
     9421 aattgctgga tgttttcgag cgcggcgaag tccgcactga gttgttgaaa gaacttgacc
     9481 ggcagcaacg taaactccag acctggattg gcgtgcctgg cgtggaccag agccgtattg
     9541 aagcattaat tcagcagtta aaagcggcgg ggagcgtatt aatttccgcg ccccgtatcg
     9601 ggcaatttct gcgtgaagat cgtttgattg ccctggtgcg tcagcgactg agcatccccg
     9661 gcggctgttg tagctttgat ttacctacat tgcacatttg gctgcatcta ccccaggcgc
     9721 agcgcgacag ccaggtagaa acctggattg ccagcctgaa cccgctcacc caggcactta
     9781 ccatggtgct ggatttaatt cgccagtcgg cccccttccg taaacaaacc agcctgaatg
     9841 gtttttatca ggataacggt ggcgatgccg acttgctgcg cctgaatctg tcactcgatt
     9901 cacagcttta tccgcaaatt tccggtcata agagccgttt tgccattcgt tttatgccgc
     9961 tggacagtga aaacggacag gtaccggaac gtctggattt cgaactggcc tgttgctaag
    10021 gagtaaaaat gtcagaaact attacggtga attgcccaac ctgcgggaaa acggtggtgt
    10081 ggggtgaaat cagcccgttt cggccatttt gctccaaacg ttgtcagctg atcgacctcg
    10141 gagaatgggc tgctgaagaa aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg
    10201 actggagcga agagccaaag cagtgacatt ttatctgatg cgacgctggc ctgataagac
    10261 gcgggcagcg tcgcatcagg catcgtgtgc aaatgtcgga tgcggcggga acgccttatc
    10321 cgacctacaa gaccttacat atcaacatgt taaaattgta gatactgata agacgcgcca
    10381 gcgtcgcatc gggcatcttg tgcaaatgtt ggatgcggca taaacgccta tccgacatac
    10441 acacgcttaa gcttcgcaag taccggttca ttggctggcg gaaaatcatc ggcattcaga
    10501 ccgaccagcg acatccactc accgggttgc ccttctttac cccacggctc cccttcccag
    10561 ctttcgacca gccagaacca cagtgttata tgcctgtccg ggaattcata ttccagtatt
    10621 tcaaatagcg aaagatgttg tggggtaatc ccgacttctt cctgaagttc acgcaccacc
    10681 gcctgttccg gcgtttcacc catttcaatt ttaccgccgg gaaactccag tttattcgcc
    10741 atgtgcgcat ctgctgcgcg acgggttata aagatttcat tgttctcgtt gcgaataata
    10801 cctaccgcaa tttgcagctt tttcattgtc ttaaacctat aaaaaaggcg cagaatcctg
    10861 cgccttttac ttcaactgtt agcttttatt gcaggcggcc atggcactgc ttatattttt
    10921 taccagaacc acacgggcaa ggatcgttac gtcctacttt gcgttcaccg gtttgcgccg
    10981 ccagtgcagc tgcggcagca gagtcgtcat cctgatggct aagctgctgc atttgcgcta
    11041 aacgctcggc ttccatacga cgctgttgtt ccagctcctc aacctcttca ggcatacgta
    11101 cctgaacttt gctcagcgtg ctgataactt catattttaa cgactccagc atcgctgcaa
    11161 acatggagaa cgattcacgt ttgtattcct gcttcggatc tttctgtgca tagccacgca
    11221 ggtggatacc ctgacgcaga tagtccatcg cagccaggtg ctctttccac agagagtcaa
    11281 gcgtttgcag catgacgcct ttctcgaagt gacgcatcat ctcagcacca accacttctt
    11341 ctttacgctg atacacttcg atggactgcg ccagaatgcg ctcacgcagc gtctcttcat
    11401 gcagttctgg ttctttatcc agccactcgg caattggcaa atcgaggtcg aaatcgttct
    11461 tcagacgttc ctgcagcccc ggaatatccc acatttcttc cagcgactgt ggcggaatgt
    11521 aggcatcaat ggtcgctttg aacacatctt cacgaatgct gttgatggtt tcgctcacat
    11581 cgctgacatc cagcagttcg ttacgctggg agtaaatggc gcgacgctga tcgttagcca
    11641 cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt acggctttca actttacgct
    11701 gggcgttggc aatcgctttg gtcacccacg ggtgctcaat ggcttcgcct ggcttcatac
    11761 ccagtttacg catcatgccg gatactcggt cggaagcaaa aatacgcatc agcgcatctt
    11821 ccatcgacag gtagaaacgg gaagaaccag catccccctg acgaccagaa cgaccgcgca
    11881 gctggttatc gatacgacgg gattcgtgac gttcagtacc gatgatatgc aggccacctg
    11941 ctgccagtac cgcatcgtga cgtacctgcc agtcggcttt aattttttca atttgctctg
    12001 cagtcggatt ttccagcgcg gcaacttctg cctgccagct accaccgagc acaatatcgg
    12061 taccacgacc cgccatgttg gtcgcgatag tcaccgcagc cgggtaacct gcctgagcaa
    12121 caatcgccgc ttcgttggca tggaatttgg cgttcaggac gttgtgctta ataccggctt
    12181 tggtcagttc atttgacacc agctccgatt tttcgatgga gattgtaccc accagcaccg
    12241 gctggccttt cgcagtgcgt tctttgatat cttcaatgat cgcctgaatt ttttccgctt
    12301 cagtcatgta gaccaggtcc ggcagatctt tacgaatcat tggacggttg gtcggaacaa
    12361 cgacagtatc cagcttatag atggagctga attcgaaagc ttcggtatca gcagtaccag
    12421 tcatccccgc cagtttttca tacagacgga agtagttctg gaaggtgatc gaagccagcg
    12481 tttggttttc gttctggatc tgcacacctt ctttcgcttc cacagcctgg tgcagaccat
    12541 cggaccagcg acggccctgc atggtacgac cggtgtgttc gtcaacgatg ataacttcac
    12601 catctttaac gatgtagtcg acgtcgcggg taaacagcgc atgagcgcgc agcgccgccg
    12661 ttacgtggtg catcagcatg atgttggccg gagagtacag agactcacct tcatccatga
    12721 tgccttcttt aaccagcagt tcttcaatca gaaccagacc acgttcggtc aggttcacct
    12781 ggcgagattt ttcatccacc gagaagtggc cttcgccctg gaaggtttcg gagtcttctt
    12841 tttcctgacg gatcaggtgt ggaataattt tattcacgcg tttatacatt tccgagctgt
    12901 cttctgccgg gccggaaatg atcagcggtg tacgcgcttc atcgatcagg atggagtcca
    12961 cttcgtccac cagcgcatag tgcagtttac gctgtacacg ttcttcagga ctgaacgcca
    13021 tgttgtcgcg caggtagtca aagccgtatt cgttgttcgt accgtaagta atgtcagctg
    13081 cgtaagcttc acgctttgcc ggtgctggca tgcccggcag gttgatacca actgtcaggc
    13141 caagaaattc aaacagcgga cggttgtttt cggcgtcacg ttgcgccagg tagtcgttga
    13201 cggtaactac gtgcacgcct ttaccggtta gtgcgttcag gtaagcaggc agcgttgcgg
    13261 tcagggtttt accttcaccg gtacgcattt cggcgatgca gcgttcgtta agaaccatac
    13321 cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac gcgcttactt gcctcacgca
    13381 ccacggcgaa agcttccggg atcagatttt ccagcacttc gcctttttcc agacgtgcac
    13441 gaaactctgc ggttttccct ttcagttctt cgtcggagag tttttccatc tccggttcca
    13501 tggcattgat gatgttgacc actttgcgca tccggcgcag ggtgcgatcg ttacgactac
    13561 cgaaaacttt agttaataat ttgattagca taataaaatc tcaaacgccc cgcgttgcgg
    13621 agttaataaa atgaagtaac ggtttattgt tgttagctga ggcgttgagg gccagcacgg
    13681 atgccttgcg cctggcttat ccagacgggc gtgctgaatt ttgcttgcgg ggtaaaatgc
    13741 gcataatcaa tgcgataacc cttttcagac ggcgtgcctt cctgggtcaa cagcgcgctg
    13801 agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca aagattcttc agcaacgggc
    13861 agcgtttgcg gtgccattgc gaaggaaaga tgacggatta ccgtgcgaat ggcatgttga
    13921 tgccagtaat caacggaata gttcgaattc gggcgacgcg tgttcgcttc cagcaaggcc
    13981 aattgaccaa agttaacttt ggctgaaggc tcgtggttgc gggttgtcgc ttttgcgggc
    14041 gcgtttggtt cggcggcgtt gctgagcgca ggcaaaccta aactcgccgc gaccatccct
    14101 aataagagat gcggccagaa gtagcgttta ccaaactgtc gccagcgcgt cagtattcca
    14161 ctcacgttat tgccatcccg atcgtattca aacgtattta gcgcacaaat cttatcatta
    14221 aagcctgaag tccacagcac gaatgaagat aagttgtgcg taaaagtgct taagaaaaca
    14281 gcgttcgcac cgttttttat cgttcttata aaattcaaaa aaaaacgact ggttcacctg
    14341 gccggagaga gtgccagatt tgccagtcga attttatacg acagtataaa tgtcgttatg
    14401 ccagtacggc tgaaggcgct ttgaaggcca acggcagttc tgcgtcgtcc tggaaggtca
    14461 catattccca ggcttcctgt ttcgccagga cagcctgcag cagtttgtta ttcagtgcat
    14521 gaccagattt ataagcggta aatgcaccaa taatattgtg accacacatg aacaagtcac
    14581 cgatcgcatc gagcattttg tgacgcacaa attcgtcttc aaaacgcagg ccgtcttcgt
    14641 tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa gctgccgccc aggcacaaac
    14701 cacgggactg cagatattcg atatcacgca tgaaaccgaa cgtacgcgca cggctgatct
    14761 ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg ctggttgctg gaatcaatag
    14821 ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc attgtacggc ttaaattcag
    14881 cccacttatc gccatcttcg acacgaacag tctctttgat gcgaacaaat ttcttggcgc
    14941 agttcagctc gtcgatacca gcgtcaagca gcaggtatac aaacggagcg gcgctgccgt
    15001 ccatgatcgg gatttccggc gcgttaactt cgataacaat gttatcgatg cccaggcccg
    15061 cgagagcagc attgaggtgc tctacggttg aaatccgtac atcatgctcg ttgaccagac
    15121 acgtacagag catggtatca cgcacagatt tggcatcggc cgggaaatct accggtggat
    15181 tcaagtcggt gcgacgatag atgaccccgg tgttggccgg cgcagggcgt aacgtcaggg
    15241 tgactttctt gccggtatgt aaaccgacac ccgtcgcctg aacgatacgt ttaagtgtcc
    15301 tttgtttgat catcgtatta tctcgccaaa ttacctatcc aaccgaagtg tactatacat
    15361 tcggcgggcc agtttagcac aaagagcctc gaaacccaaa ttccagtcaa ttcttaatca
    15421 gcttgcttac gcaggaatgc tgggatatcc agataatccg gctctttcgc agtttgcggc
    15481 gcattgtcat tcacgacttt agcaaccggc ttctgctcct gggtcagcgg agccatcccg
    15541 tgctgctggt agcgatccat cactggctgc tgaacctgct tattggtcac cagagtgatt
    15601 tcaggacgtt tgtccatgcc gatacctgtc gcaacaacag ttacgcgcag ctcgtcattc
    15661 atatccgggt caagagaagt accgataacc acagtcgcgt tgtcggaagc aaatgcacgg
    15721 atggtgttac ctaccgtttc gaactcatcc agacgcaggt cgaagcccgc tgtgatgttt
    15781 accagtacgc cgcgtgcgcc agacaggtcg atatcttcca gcagcggaga agagatagcc
    15841 atttcagcag cttcttccgc acgatcttca ccgctcgcca caccagaacc catcattgcg
    15901 tagcccattt cagacattac ggtgcgtacg tctgcaaagt ccacgttcat caaacccgga
    15961 cgagtaatca gttcggcgat accttgcaca gcgcctttga gtacgtcgtt cgctgcgcca
    16021 aacgcatcca gcagggagat accgcggccc agaactttca gcagtttgtc gttcgggata
    16081 gtgatcagag agtccacatg cttggacagt tcagtgatcc cctgctccgc gaatgccata
    16141 cgcttcttgc cttcaaagtt gaagggctta gtgacgacag caacggtcag gatacccaaa
    16201 tcttttgcca cttcagcgac gactggagct gcgcctgtac cggtaccacc acccataccc
    16261 gcagcaataa agaccatgtc tgcaccttcc agcgccgcac gcagtgcatc gcgatcctca
    16321 tcagccgcat tgcggccaac ttctggatta gcgccagctc ccagtccttt ggtgataccg
    16381 ctaccgattt gaatcgtctg tccaaccgct gttttacgca gcgcttgtgc atcggtattt
    16441 accgcgaaga attcaacacc ttcaatgcgc tcgcgcacca tgtgttcaac agcattaccg
    16501 ccgccgccgc cgacgccgat gactttaatc accgcgtcat tggtaagttc cattggttca
    16561 aacatagttt ctctccgatt tgtgcctgtc gcctgagacc gtaatcattg tcggcctcat
    16621 aaaaattaaa actcttttcg cagccaacta ttgagtcgct tgatccacga gccaactgat
    16681 gctgtaacac gtttttctac ttcagcttca ccgttaagat gtgactcttt cccgtaatga
    16741 agcaatccca ccgccgtcga ataatacggc tcctgagcat aatccgttaa accggtaatg
    16801 ttcagcggcg cgccgatacg cacctgcgta tgaaacacgc gctgagcaca ggctgcaaga
    16861 ccttcaatct gcgctgcgcc accggttaat acaatgcctg ccgccaggtg atgtttaacc
    16921 ccttgttggc gaagcttttc ctgcaactgc aatatctctt cgttgaccag gttgagcagc
    16981 tcggtatagc gcggctcgat cacctctgcc agggtctgac gttgcagact ccgcggcgga
    17041 cgaccgccta cgctcggcac ttccacgctc tcatcttttc caacgatgga acccagcgca
    17101 caaccgtggc gaactttaat cgcttcggcg tcgcttggcg gcgtgccaaa ggcgtaagcg
    17161 atatcactgg tcacgacatt gccagcataa ggaattacct tagtgtggcg caatgcccca
    17221 ccggtataaa cggcgatatc cattgtacca ccaccgatat cgacgacgca gacacccagt
    17281 tcacgttcat cttccgtcaa taccgaataa cttgatgcca gtccggcaaa tatcagttgg
    17341 tcaactttca gcccacaacg ttcaaccgct ttgacgatgt ttttcgccat atcgttgtga
    17401 catgtgatca ggtgcacttt tgcctgcatc cgcacgcccg aaagtcctac cggattcttg
    17461 atcccttcct gatagtcaat cgcatactct tgcgggatca catgcagcac acggtgttca
    17521 tcgcgcacac gcaccgattt cgcggtatgg acaacgtttt ccacatcttc ttgcgtcact
    17581 tcttcttcag aaataggcac cataccaatt tcattctggc agctgatgtg cttaccagaa
    17641 agcgccagat ataccgaaga gatctgacaa tctgccatca attctgcctg gtcaatggcg
    17701 cgctgtacgc acttgaccac ggattcgagg tcgttcaccc cgcctttatc cataccacgc
    17761 gacgggcagc tgcccacgcc aatgatattg accataccgt cgggcagaac ttcccctact
    17821 aaagcggcaa ccttcgcggt accaatctcc agtcctacta ccagttttct gtccgtcgcc
    17881 ttaatcattg ttgttctgcc tgtgcctgat tttgttgctg agtagattcc tctggcggca
    17941 agggcgccca gcctactgcc gctccagagt cataacgcaa atcaacgtag ctaatccgtt
    18001 tgccatcggt ttgcgcctgc tgctgtaaaa ccggataaag ttctacaaag cgagccaaac
    18061 gtttcatcgt atcgccccgg ccaagattga gcttaatatc gttattcagc gtcaactgcc
    18121 aggaacgccg cgcggtcatc gccgcttcct tcagagtaaa tctgtccttt gccagcatct
    18181 gccccatttc gcgatagccc tgcaacactt cattggcgct gccttccggg ccatacagca
    18241 ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct gaaagtgttt ccttccgcgt
    18301 ctaccatatg ttgatcattc caccgcgcaa tcggcacata ttcaaccaga tgaatcttca
    18361 attcatcagg ccactgcttt ctgacgctca cctgcttaat ccacggcagg cgttgttcta
    18421 tttgcgtctg gatgatgttg acatcctggg tcataaaggt acctggctca cccaatgcca
    18481 ggatcgactg ccggatatcg tcattacgcg tgtaatggcg ttcaccggtc aacaccagct
    18541 ttgagagcgg caggcgttgc gcatcttcca tccagcccaa cacgacccag ccgctcacca
    18601 acactgtcgt taaaacggtc agcaggaaaa ggatccccgc cagacgcgtt ccattattgc
    18661 ggcgagaaga aacctcttct tcgctgtttc gcgtgttcag agcagcctgc gacatattag
    18721 tccgccagtt ccagaattcg tactaccaac tgcgagaagc tcatacctgc ctgacgtgcc
    18781 gccatcggca ccaggctgtg gctggtcata cccggtgagg tattggcttc cagcagataa
    18841 aactgtccat cgctgtccag cataacgtca atacgtcccc atcctttgca acctaacgtc
    18901 gtccatgctt tcagcactaa tgcctgcaaa ttggcctctt gtgacgcttc cagacctgcg
    18961 gggcagaaat actgtgtctc atcagagaga tacttcgcct cataatcata gaaggttccg
    19021 gacggttgaa tacgtattga cggtaaaatt tcttcaccga gtatcgcaac cgtgaactcc
    19081 ggcccactta gccatttttc aatcaatact tcttcatcgt gctgaaatgc caatcttaat
    19141 gcatcttgta gagcattttc tgctactact tttgacattc ccacactgga accttcgcgg
    19201 ctcggcttaa cgataaccgg caaacccaga gcagaaattt ctgctaactg cttatcactc
    19261 aggccttttt caaactctgc gcgggttaac gctacccacg gagcgaccgg taaaccggca
    19321 ccttgccaca gaagtttgct gcgtagttta tccattgaaa gcgcagatgc catcactccg
    19381 ctgccggtat aaggcaagcc catcagctcg agcatgccct gcagcgtgcc atcttcaccg
    19441 ccgcgaccgt gtagcgcgat aaacactttc tgaaagccca tcgacttcag ttgcgtcacg
    19501 tcgacttctt tcgggtcgac aggattcgcg tcaataccgc cttcacgcag cccggctaac
    19561 accgctgcgc cagaattcag agaaacttcc cgctcagcgg aggtcccacc caacaggacc
    19621 gcgattttat cagtcatgtt gttcttcctc cggagtttgc ggcttcagtt tgatttcagc
    19681 taaagaacgg gcaatttttc cgatattacc agccccctga acgagaatca ggtcgttacc
    19741 ggttaatacc ggagccagca tctcggctac ctgcgccggg tccggcacca gaatgggatc
    19801 aattttccca cgtccacgaa ttgtgcgaca cagcgaacgg ctgtccgctc ccggaattgg
    19861 cgcttcgcca gccggataca cttccagcat caacagggta tcaacctgcg tcagcacatt
    19921 ggcgaaatca tcatacaggt cgcgcgtacg ggtaaaacgg tgcggctgaa acagcattac
    19981 caggttttta tccggccagc ctgcacgcgc cgctttaatg gtggcgtcca cttccgtcgg
    20041 gtggtggccg tagtcatcga ccagcattgc cgtaccgctt ttaccattca caggctccag
    20101 cgggaattca ccgaggaaat caaaacggcg accagtcccc tggaagctct ccagcgcacg
    20161 caaaatagcc tcgtcgtcaa ttccctcttc cgtagcaacc gcaaccgcag ctgcggcgtt
    20221 cagcgcgtta tgacggcctg gcgcattcag ggtgacgcgc atcggctctt tgtcctggcg
    20281 cagcagcgta aagtgcccct gcgggccaat ctgctgataa tcttctacac gcacgtcggc
    20341 atcttcgctg aagccgtaag tcgtggtctg acgtcccaca cgcggtaata attcgcggat
    20401 caccggatca tcaacacaca tcaccgcacg accgtaaaac ggcaggttgt gcagaaaatt
    20461 aataaaggtc tgttttaaat tctcaaagtc gccctggtag gtatccatgt ggtcggcttc
    20521 gatattggtg acaatcgcca ccatcggttg cagatgcagg aacgatgcat cactctcatc
    20581 tgcttcggca atcaggtacc gaccatgccc caaacgcgca tgaacgcccg ccgcttttac
    20641 cagcccaccg ttaacgaagg ttgggtcgag tcccgcttct gcgtagatgc tggaaaccat
    20701 cgcagtggtt gtcgttttgc cgtgcgttcc ggcaatggcg atgccatgac gaaaacgcat
    20761 taactcagcc agcatttcgg cacgacggat caccggaata cgcgcttcat gagctgcgac
    20821 aatttccggg ttatcggcag aaatcgcgct ggaaacaacg accacgctgg catcacgtac
    20881 gttttccggg cgatggttga aataaatcgt cgccccaaga ttcattaact gctgcgtgac
    20941 cggatttggc gctaaatcgg aaccactgat ctgataacct tcattggcca gaacttcggc
    21001 aataccgccc ataccggcac caccgatgcc gacaaaatgt atgtgccgaa cgcgacgcat
    21061 ttcgggcacg atggaacgca gttttgccaa ttgttgtgta ttcattcttt acgccattaa
    21121 cttcttaaat tcatacgatg caaaaggcat cgctacaatt acgcccgggc agcccggctc
    21181 acttcatttg ccactcgctc ggtggcatcc ggaatggatg cggcgcgggc gcgttctgcc
    21241 atggttaata aggtttctcg cgaccacccg gccagggtgt tggcgacagc atccacgcta
    21301 agctgtggct gctcgataat tttggctgcg cccgcttttt ccagcggtag cgcattccag
    21361 tattgctggc ggtctttatg ttgaaacggc acaaacaacg ccggtagtcc tgccgcggcg
    21421 atttcactca ccgttaacgc accggagcgg caaacgacga catccgccca cgcatacgcc
    21481 gccgccatat catcaataaa ttccgtcact ttatgctgcg gttgccccgc ttcggcatac
    21541 gcctgttcaa cggattgttg cgaaccttta ccgctctgat gccagatagt gactgaatca
    21601 cccagtttcg cagcaacctg cggcattgtc tggttaagaa tgcgtgcgcc ctgggaacca
    21661 cccactacca acacacgaac cggaccttca cgtccagcca aacgttgctg cggcaacggc
    21721 agcgccagca catcggtacg caccgggtta cccaccactt ccgcattagg gaaagcgcct
    21781 ggaaacgcct gcatcacttt ggtggcaatc ttcgccagcc atttattggt taatcccgca
    21841 ataccgtttt gttcatgaag cacaaccgga atacctaacg accacgcggc cagaccacct
    21901 ggacctgata cgtagccgcc cataccgagc accacgtcag gtttgtacgc tttcataatc
    21961 gcccgcgcct gacgccaggc gttgaagata cgcagcgggg cagctatcag tgcttttata
    22021 ccttttccac gcagaccaga gatacgaatg aaatcaattt cgatgccatg ttttggcact
    22081 aagtccgctt ccatacggtc ggcagtcccc agccagcgaa cttgccaacc ctgagccatt
    22141 agatggtgcg caaccgccag tcccgggaat acatgtccac cggttccgcc tgccatcacc
    22201 attaatcgct tcccttgacc actcatcgtg aacctcgtac aaacgcctgc gctttctcca
    22261 gacgcgtttc ataatcaata cgcaacagca tcatgatggc tgtcgacata atcagtaagc
    22321 tcgaaccacc gtaactgatc agcggcaatg tcagaccttt ggtcggtaac atccccgccg
    22381 ccgcgcctac gttaaccagc gcctggaaac taaaccagat accaatagaa caggcgagaa
    22441 aaccggaaaa acggtggtca atttctaatg ctttgcggcc aatcgacatc gcgcgaaaag
    22501 cgacgaagaa taccattaaa agtgccagca ccacaccgac ataccccagt tcttcgccga
    22561 taatggcgaa aataaagtca gtgtgcgctt ccggcagata ctccagtttt tgtaccgagt
    22621 tacctaaacc ttgtccccac agttcaccgc gaccaaacgc catcaacgat tgcgttaact
    22681 gatagccgct gccaaaggga tcttcccacg gattccagaa tgcggtaaca cggcggatac
    22741 ggtacggttc ggcgaggatc agcaacacaa ccgctgaaat gcccataccg ataatggcaa
    22801 tgaactgcca caatttcgct cccgccagga acaacatcgc cagcgtagtc acaaacaaca
    22861 ccaccaccgt accaaggtct ggctgtgcca gcagtaacac tgccaacacc agaattacgc
    22921 ccatcggttt caggaagccg cgcaggttat tacgtacttc gtcgccttta cgcaccagat
    22981 agttggcgat atagcaaaac agcgacagtt ttgtcagctc cgcaggctgg atacgcagca
    23041 aaccgagatc gatccaacgc gatgcccctt taaccgagct acccactacc agaacgatca
    23101 tcagcaggat gatagatccg agcagcatcg tggcactgta gcgttgccag aactccatcg
    23161 gcagacgcag cgtaatgatc gccagaatga atgccaggat cagatagacg ccatcacgct
    23221 tcgcgaagaa gaacggatcg ttggttaagc gttgccctat gggcattgac gccgaggtca
    23281 ccatgataaa gccaatcgcc gcgaggccga aggtcagcca cagtaaggtg cgatcgtaca
    23341 tgatcaggct gtcggtatct ttttcccgcg agcccatcac ccagcccttt agcgccgtgg
    23401 agatccagac cagaatactg aatcctggca ggcgcggcat tttcaggcga gggagagata
    23461 aacgcatcaa cctaactcct tcgccagacg ggcaaactca ttgcctcgtt gttcaaagtt
    23521 cttgaattga tcaaggctgg cacaggccgg ggagagcaga accatatcgc ccggctgaac
    23581 acgcggagcc agcaagcgca tcgcctgttc catggtttcg gtttgttctg ccacttccgg
    23641 gcgtagcgcc gccagctgcg cgccgtcccg accgaaacaa tacagacgta cattatcgcc
    23701 attcaggtaa cgcgccagtg ggctaaagtc cgccgattta ccatcgccac ccagcaacaa
    23761 atgcagcgtg ccgtctacat gcaggccatt cagcgccgct tccgtactgc cgacgttggt
    23821 cgcttttgaa tcgttaaccc agcgcacacc gttatgctcc agcacaactt caaagcgatg
    23881 cggcagacca gtgaatgtgg ttaacgcttt caggctgctg gcacgcggta accctgcggc
    23941 atctgccagc gccagcgccg ccagcgcatt ggtgtagtta tgctgcccgg aaagtttcat
    24001 ttctttcacg ttcagcactt tctcgccttt cacccgcagc caggtttcac cctgctgatg
    24061 attcaggtga tagtcgccca tgttgacgcc aaagctgacg cagcgttcat ccgcaccgcg
    24121 aatcggcatt gttaaggcat catcagcatt aaccacgcaa atttttgcgt tttcgtaaat
    24181 gcgcagtttt gctgcacgat actgttgtaa accaaacgga tagcgatcca tatgatcttc
    24241 agtcacgttc agaatggtcg ctgctaccgc ctgtaaactg gaggtggttt caagctggaa
    24301 gctcgacagc tccagcacgt acagttcgca ctcatcatcc agcagcatca acgcaggcag
    24361 gccaatattg ccacccacac caacgttaac ccccgccgct ttcgccattt cacccactag
    24421 cgtggtgacc gtgcttttgc cgttagaacc ggtgaccgcc acaatcggtg cttgtgcttc
    24481 acgacagaac agctcgatat cgccaacgat ttcgattccg gcatcagcgg cagcgcttaa
    24541 ggatggatgc gccagtgcaa taccgggact ggcgacaatc agatcggccg ccatcagcca
    24601 ttcatcattc agaccgcccg tgtggcgttc tacagcttcg ggtaatttat ccaggccagg
    24661 cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg cgagcgagga aaaagtccac
    24721 gcaagaaagc ccggtgaggc ccaggccgat aatgacgaca tttttaccct ggtaatcagc
    24781 catgattaac gtaccttcag cgttgccaga ccaatcagga ccagcatcag cgaaataatc
    24841 cagaaacgta caatgacgcg cggttccggc cagcctttca gttcatagtg gtgatgaatc
    24901 ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg agccgacctg caggatgacg
    24961 gaaagtgttt ctaccacgaa cacgcccccc ataatcacca gcaggaattc ctgacgcagc
    25021 agtacggcga taatacctaa cgcaccacct aacgccagcg aaccgacatc gcccataaag
    25081 acctgcgccg gataggtgtt aaaccacagg aagcccagcc ctgccccgac tatcgcggta
    25141 cagacaatga ccagttcccc ggcgtgtcgt agatacggta tgtgtaaata gctggcaaag
    25201 ttcatgttgc cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa tacggtcggc
    25261 ataattgcca gaccgtcgag accatcagtc aggtttaccg cgttgcctgt acccacaatg
    25321 acgaagtaag ccagcagaat gtagaacagc cccagttgcg gcatcacatc tttaaaaaat
    25381 gggaccacca gctgcgttgc gggcgtgtct ttgccaacaa ggtacagcgc gaaggcgaca
    25441 cccagcgcaa tgaccgacat ccagaaatac ttccaacgag cgatcaaccc tttggtgtct
    25501 ttacgcacta ctttgcgata atcatcaaca aagccaataa caccgtaacc taccagcact
    25561 accaacacgc accagacgta cggattggac gggtaagccc acagcagtac ggagatcaca
    25621 atcgctgtca ggatcataat cccgcccatg gtcggcgtac cgcgcttgct gaagtgtgat
    25681 tcaggaccgt cgttacgcac cacctgacca aaggaaagtt tttgcaaatg agcaatcatg
    25741 cgcgggccca tccacaatga gatgaacagc gcagtcagca ggctgacgat ggcgcgaaac
    25801 gtcagatagg aaaagacgtt aaagccggaa taatatttga ccaaatgttc ggccagccaa
    25861 actaacatgt cccattctcc tgtaaagcgc gtactacctc ttccatggca gcactacgtg
    25921 aacccttaac taaaatcgta attacctgtt gctcagcaat cagtgattta agacgcgcga
    25981 taagcgcagt tttatcggaa aaatgttcgc caacaccgct ggcagtgctg atagcatggc
    26041 tttgtttgcc cacgcttaac acgcggtcaa taccagctgc ttttgccgct tcgcccacct
    26101 gtatatggca ggcttcgctt tcagcgccca gttccgccat atcgcccacc accagcacgc
    26161 ggtagcccgg catttcagcc agtacctgga ctgctgcggt cattgaaccg acattggcgt
    26221 tgtaggagtc gtcgagcagc aactggtttt ccgccagttg gatggggaac agacggcctg
    26281 gaacagcttt cagatttgcc agccccgctt tgatagcatc aagcgttgcg cccacggaca
    26341 tggagagcgc agcggctgcc agcgcattcg caatattgtg acgccccggc aacggcagca
    26401 gaacatccac gctaccggtt ggggtttgca gggtaaattc cgtaccgtgc gaagtcacat
    26461 ggatgttggt ggcggtgaaa tcgctgttgg cggcattagg tgagaaacgc cacactttgc
    26521 gtgagccaat tacgctctgc cagttcagcc agtcgttgtt gtcggcgttc ataatggcga
    26581 taccgttttc cggcaggccg ctaaagattt cacctttcgc tttcgcgaca cccgcaagcg
    26641 agccaaaacc ttccagatgc gccgctgcca ggttgttgac cagcgccgct tccgggcgag
    26701 tcagactcac agtccaggca atttcgccct gatggttcgc gccaagttca attactgcgt
    26761 aatcgtactc tggcgttaag cgcaacagcg tcatcggtac accgatgtcg ttgttgagat
    26821 tgcctgccgt ataaagcgtg ttgccacact ggcttaaaat cgctgccgtc atctctttaa
    26881 cggaggtttt gccggaggaa ccggtcagag caaccacgcg cgccggaact tgctggcgaa
    26941 cccatgcagc cagttcacca aacgccagac gagtatcctt gacgattacc tgcggcagat
    27001 cgatatccag cggacggtta accagtagtg cacccgcacc gccagctttc gcctggtcag
    27061 caaaatcatg ggcatcgaaa cgttcgcctt tcagggcaac aaacaggcaa cccggcgtca
    27121 gttttcgtgt gtctgtggtt acagcatcaa gggtgatatc tgcccctttt agttcaccgt
    27181 tgagaatatc ggtaagttgg ctaagggtta cgctaatcat gcaatcaccc ccagcagacg
    27241 cgccaccgtg acgcgatcgg agtagtccag acgctgattg ccaacaatct ggtaatcctc
    27301 atgacctttg cccgcgacca gtaccacatc attctcttta gcctgcataa cggcgcaagt
    27361 caccgcttca gcacggcctt ccatcacttt ggcatgtccg gcatctaaca ttcccgccag
    27421 aatatcgttg atgatggcac gcggttcttc ggtacgcggg ttatcgtccg tcaccaccgc
    27481 cacgtcagcg aactcttcag caattgcgcc catcagtgga cgtttgcctt tatcacgatc
    27541 gccaccacag ccaaagacgc accacagctt gcccgcacag tgcaggcgcg ccgcctgtaa
    27601 ggctttttcc agtgcatccg gtgtatgcgc gtaatccacc actaccgtcg gtttgcctgg
    27661 cgcagtgaac acttccatac gtccacaaac cggctgcagg cgtgcggcag ttttcagcag
    27721 gtcagccaac ggatagccca gcgccagcag cgtcgccagc gccagcagca agttgctgac
    27781 gttaaaagcg cccatcagat ggctttcaat ttcgccatcg ccccagcttg agctaaagcg
    27841 aatcgtcgca ccgctgtcgt gatagttcac ttctgtcgct ttcaaccagc gtccgtgaca
    27901 gttcggattg atatgatctt ccattgatac cgcaaccgcg tccggcagtt ttgccagcca
    27961 gcggcggccc acttcatcgt cggcgttaat aatcgcctga ccgcaatgat gctcagaata
    28021 aagcagccat ttcgcggctt cgtagtgttc catatcacca tgataatcaa ggtgatcgcg
    28081 gcttaagttg gtaaagaccg acgccgcaaa tttcaatgcc gccacacggt gctgtaccag
    28141 tccgtgggag gaaacttcca ttgcgcaaaa agtcgcgccc tgatccacca gccccgccag
    28201 ctcatgctga acatcgactg ccgaaccggt tgtattttct gtcgggatca ctttccccag
    28261 caggccgtta ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt ggctccactg
    28321 cgccagcagc tgggtcgtcg tggttttgcc gttggtgccc gttacgccca cgagacgtaa
    28381 attgtcagag ggttcatggt aaaagcggcc cgccagtgca gataaacgct cgttgagctg
    28441 gctgagatag atgaccggta cgccgtgcat ttcacggatt tcaccgtcgg tcgcctcatc
    28501 tttcgcctct gcaataatgg cagccacacc ttgcgctatc gcctgcggga tatatcgacg
    28561 cccgtccgcc tgatgaccta ctacagctac aaagagatcg cccgccgcag ccacacggct
    28621 gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct ggcacccacg gagcaagaag
    28681 gtcgcgcaaa ttacgatctg ccacctgtcc cctcgccttg attaatcaca aattcatttt
    28741 tatcgcccgt tgtcagcgca tccggttcga tgttcatggt acgcaatacg ccgcccatga
    28801 tggcaccaaa gaccggcgcg gaaacggcgc cgccgtagta tttacccgcc tgcggatcgt
    28861 tgataacaac aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct gcggtataag
    28921 caatatattt attgatgtag cgaccgtccg gcccgacctt tttcgcggta ccggttttaa
    28981 tggcgatacg atagccttta atcgccgcct tcacgccgcc gccgcccggt agcgccacgc
    29041 tttccatcat atgcaccacg gtgcgaacaa tggattccgg gaagactcgt tcaccgggaa
    29101 ccggggggtc aactttggta atcgacagtg ggcgataaat gccgtagctg ccgatagttg
    29161 cgtagactcg cgctaactgt aacggtgtta ccattagccc gtagccgaaa gagaaggtgg
    29221 ccctctctat gtcagaccac cgttgttttt gaggatataa gccactgcgt tctccgacca
    29281 accccaaatt ggtcgctttt cccagtccaa aacgtgagta agtatctact aacgctgagg
    29341 acggcatcgc taacgccagc ttggaaacac cgacgttact cgacttctgt aataccccgg
    29401 tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc gtggccgtta attcgataag
    29461 gaacggtatt cagtaccgag ttttcccgca ctacgccacg ttgcaacgcg gtcatcacca
    29521 ccatcggttt aaccgttgaa cccggttcaa acacgtcagt gatggtacgg ttacgcatcg
    29581 cctctttcgg cgtgccactc agattgtttg ggttgtatga cgggctgtta gccatcgcca
    29641 gcacttcacc ggtgttgaca tccaccagca cggcgctacc agattcagct ttgttaaagg
    29701 ccaccgcgtt gttcagttcg cgataaacca gcgcctgcag gcgttcatca atactcagcg
    29761 ccaggttgtg cgccgcctga ctgtcggtgg aagagatatc ttcaattacg cgaccatagc
    29821 ggtctttacg cacaatgcgc tcacccggct gcccggtaag ccatttatcg aaactcttct
    29881 cgacgccctc aatcccctga ctatcgacgt tagtaaagcc gatgaggtga gcggtcactt
    29941 cgccggatgg ataataacgg cgagattctt cacgcagatg aatccccggc agtttcagtt
    30001 ttttgatgta ctctgccatg tcagggttta cctgacgcgc cagataaata aagcgccctt
    30061 tcgggttggc gttaatgcgg gctgaaagct gatccagcgg aatattgagt gcgttagcca
    30121 gcgccttcca gcgatcaccg acgctgatac cgccagcgtc atgcacttct ttcgggtcag
    30181 cccaaatcgc ttttaccggc acgctcaccg ctaacgggcg accagaacgg tcagtaatca
    30241 tgccgcggga ggtggaaact tgctgaacgc gaagagaacg catgtcgccc tcttttacca
    30301 gcatatccgg ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa gccagcgcca
    30361 ggagaataca gccgcataac aacgcaaaac gccaactgat aaagttggca tgttcttcct
    30421 gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat ccttattttt
    30481 gcactacgat attttcttgt gacggatcaa catgctgcat ttgcagcttt tccgtggcga
    30541 tcctttccac ccggctatgg tcgccgagcg cattctcttc aaggatcagg ttgcgccatt
    30601 caatgtctaa agcatctcgc tccagcacca gttgttcgcg ctgtgcggtc agtaaacggg
    30661 tatggtgcgc cgtggttacc acggtcaccg ccgtcaaaat aatgcaaatg aacaggcaga
    30721 gtggcagctt cccaaatcgc aaaagatcgt caccgataac accaggcaat gcatggcgct
    30781 cgtggcttcc catcgatcct ttaactttgc ttagagcttc tgtcactctg ctgatcatgc
    30841 gttcgtcctc tctgcaatac gcagaactga actacgggca cgagggttct cagccacctc
    30901 ttcttcaccc ggcattaact tgcctagtgc tcgcagctga cggccaccca gttttttgag
    30961 ctgctcttca gtcatcggta accctgccgg aacttgcgga ccgcggctgt tttcacgcat
    31021 aaaacgtttc acaatacggt cttccagcga gtggaagctg atgatcgaaa gccgcccacc
    31081 cggggccagc acgttgagcg agctttttag cgcctgctct atctcctcca gttcactgtt
    31141 tacccaaatg cgcaccgcct ggaaggtacg ggtcgcggga tgtttaaact tatctttcac
    31201 cggcgttgca gcagccacga cttccgccag ttctttggtg cgggtcatcg gctgttcgcg
    31261 gttacgctcg acaatggcgc gggcaatgcg tttggcaaaa cgctcttcac cataggtttt
    31321 caatacccag gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg ctgactgccc
    31381 acgggttggg tccatacgca tgtccagcgg accatcgcgc ataaaggaaa agccgcgttc
    31441 agcatcatca agttgcggtg aagagacgcc aagatcgagg agaatgccgt cgatcttgcc
    31501 gataagatcg cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt ggatgatgga
    31561 gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc gatcgatcgc
    31621 cagcaaacgc ccctcttcgc caagctgcga gaggatcaga cgtgagtgac caccgcgacc
    31681 aaaagtccca tcaatgtaga tgccatcagg acggatattg aggccattaa cggcttcatc
    31741 cagcagcacc gtagtatgtt tatagttttc catcatttta tagagacaag tcctgcagtc
    31801 gctccgataa gtctccagta gccaactgct ctgcgtcgat atcttccttg acctgttgat
    31861 gccaggttgt ttcatcccac agctcaaact tgttgaactg tccaaccagc atcacttctt
    31921 ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa caatcgacct gcgccatcca
    31981 tctgacattc gctggcatga cctaacagta ggcgctgcac acggcgctca accgggttca
    32041 tgctcgacag acgcgataat ttttgctcga taatttccca ttcaggcagg gggtaaagca
    32101 gcaggcacgg gtgatgaatg tcaatggtgc aaaccatttg accggcagcg ttctcaagca
    32161 gctgttcccg ataacgggta ggcactgata agcgcccttt gctgtcgaga ttgactaacg
    32221 ttgctccccg gaacatgcca gcctcacccc ttattcccac tttgccccac aatttcccac
    32281 tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg cataccgagg
    32341 taaaaaaccc gatgtttacg gctaatatca gcgttgttta ttttacgtcc aacgtgcgaa
    32401 gcaaattaac gttatgaata gctgtaagaa aaaatccctc atcgtcatca ccgttttaaa
    32461 atgactcact attttccaca gcaaatataa agtgtcagtt tgcgacgcga gcggcatttt
    32521 aggacatatc ttccccggtt aacagtccct gttgcgtctg tgtggcgctt gatgccagcg
    32581 ggtttgcgca aaagcaaaga aaagtgtttg ttaaatcggc aattcatctc tgcatgtaac
    32641 aaaataatgc aaaaacgcgc tatcgatatc acttaattat tgtgaatttc agctggtgag
    32701 gtaattaacg ggcaggttta aaagcattta tcaataatag ccaggaatag tcttatttac
    32761 tttaaggata ttgatgtcca ttcccgtact caacgcgcca gagagaaatt cacctggcgc
    32821 gtatttttgt tcgcggctta gctacggctg agcacgccgc ggcgatagag attacgttta
    32881 atgcgcgtta aaccaggttt tggcttacgc ggttcgtcca ggcttgccag gacaatctcc
    32941 agcacacgct ctgcgacatc gcggtgacgt tgagccactg ccagcaccgg gcactgtaag
    33001 aagtcgagca gttcgttatc gccaaaggtg gcaattgcca ggtcagaagg cagtttgccg
    33061 tcacgacgta gcgtgacatc catcacccct tgcaacaacg caaacgacgt tgtgaacagt
    33121 gcctgcggca tcggatgcgt ttccagccat ttttcgaata actgggcagc cgcctcccgc
    33181 tcatagctgt tggcataaag gaaatgcact tcgcgaggat catctttcca ggcagtacgg
    33241 aaaccttgtt cacgcaggaa gctgacagaa agctccggca gcgcgccaag ataaagtacc
    33301 gtctcggcgg gaaacttacg taactcttcc gccagcattt cggcatcatc ctgatcggca
    33361 ccaaccacgc tggtgaagtg ttcacgatcg agggcgcggt ccagcgcgac aatcgggaac
    33421 gggtcgttag cccagcgttg ataaaaagga tgctcaggtg gcaacgacgt cgaaacaata
    33481 atggcatcaa cctgacgctg taaaagatgc tcgatacaac gcatttcgtt gtcgggctga
    33541 tcttccgagc aggcaatcag cagttgataa cccctttgcc gcgcctggcg ttcaagatag
    33601 ttagcgatgc gggtatagct ggtgttctcc agatcgggga tcacaagacc aatagaacgt
    33661 gtgcgtccag cacgaagccc agctgccacg gcgttcgggt ggtaattgtg ctcacgcacc
    33721 acagccatga ctttttcaac ggttttgtcg ctcacacggt attgcttcgc tttgccgtta
    33781 ataacatagc ttgcagtggt ccgcgacact cccgccagcc gagcgatttc atccagtttc
    33841 acaattgccc cttgcgtaaa atgtaaaaac catagccatt gtactggtat gggttaaatt
    33901 cattaacatc taagcgcaga aaattaactg cggcaactgc ttttattcca ggttcccact
    33961 gatttcgcaa aaaaagccca acgtgacatg ttgggctgca aattgcgcat tgagatcatt
    34021 ctcaacgcat tattttatcg ccgcgcgaaa gtccgactac accagagcga gcaacttcca
    34081 caattttcgc cacatcgcga atcgatgcta aaaatgcatc aagcttatcg ctggtgcctg
    34141 ctaattgaac ggtataaagc gagggtgtga catcgataat ttgcccacgg aatatttccg
    34201 tattacgttt cacttcatca cgcccgtaac cgctggcctg aattttcacc agcatgattt
    34261 cccgctcaac gtgcgcgccc tgccccaact cgctcacgcg caagacatcg accagcttgt
    34321 gtaattgctt ttcgatttgc tcaagcactt tttcatcgcc cacggtctgg atggtcatac
    34381 gcgataatgt tggatcgtcg gttggcgcaa cggtcaggct ttcaatgttg tagccacgct
    34441 gggaaaaaag gccaatcacg cgggataacg cgcctgattc attttcgagt aagactgata
    34501 atatccggcg cataatcagg ttctctccgt tttgcttaac cacatttcat ccattccgcc
    34561 cccgcgaatc tgcatcgggt agacgtgctc actgccatcg acggtgacat caacaaacac
    34621 caggcgatta ttgcgcacct gttccagcgc ctcgctaagt ttgctttcca gttcttgcgg
    34681 atgagagatc tggatcccga cgtgaccata ggcttccgcc agacgaacga aatcgggtag
    34741 tgactgcata taagattgtg aatgacggcc ggaatagatc atgtcctgcc actgtttcac
    34801 catccccaga tagcggttat tgagattcac caccagtacc ggcaactcgt attgcaacgc
    34861 tgtagacagt tcctggatgt tcatctgaat actgccgtcg ccagtgacgc aaaccacggt
    34921 ttcttctggc aacgccattt tgacgcccag tgccgcaggt aaaccaaaac ccatcgtgcc
    34981 gaggccgcca gagttaatcc agcgacgcgg tttgtcgaat ggataataaa gcgcagcaaa
    35041 catctggtgc tgcccgacat cggacgtcac gtaagcgtcc ccattagtca accgccaaag
    35101 agtctcgatc accgcctgcg gtttaatctt ttcactgtga gtgtcatatt tcaggcactg
    35161 acgagcgcgc cactgttcaa tctgctgcca ccagtcgcgg atctcatcca gcggttgatg
    35221 gacggattct tgcgacagga gttcaagcat ttgttcgagg acctggcgag catcccccac
    35281 aatcgggata tcagcagtca cggttttaga aatggaagta ggatcaatat cgatatgcag
    35341 aaccgtggca tttgggcagt acttcgccag attgttcgtc gttcggtcat caaaacgtac
    35401 cccgacggca aaaatcacat ccgcgttatg catcgtcata ttggcttcgt aggtaccgtg
    35461 cattcccaac atgcccagtg cctgacgatg cgttgccgga aacgccccca gccccattaa
    35521 tgaggaaaca acgggcagat tcaacgcctc caccgtttct tttagctgct gatgacagcc
    35581 cgccgtgatt gccccaccgc ctacatagac aaccggtttt tttgccgcca ccagcgtttg
    35641 cagagcgcgc ttaatttgcc ctttatgccc ggaggtcgtg ggattgtaag aacgcatact
    35701 gaccgactcc ggccagacat agggtaattt gttcgccgga tttagaatat ctttcggtaa
    35761 atcaacgacc acgggtccag gacgaccact tgccgccagc cagaaagcct ttttcagcac
    35821 ctgcggaatg tcttccgttt gcttaaccag aaaactgtgt ttaaccaccg ggcgagaaat
    35881 ccccaccatg tcgcactcct gaaaggcatc gtagcctatc aacgaggtcg ctacctgccc
    35941 ggaaaggaca actaagggaa tggaatccat ataagcggtg gcaatgccag taatcgcatt
    36001 ggtcgcccct ggacccgacg ttaccagcac gacgccgact tccccggtcg cgcgcgccag
    36061 accatcggcc atatgcaccg ccgcctgctc atgacgaact aaaacatgat cgataccacc
    36121 cacggtatgc aatgcatcgt aaatatcaag gaccgcgcct ccgggataac cgaatacttg
    36181 tttaacgccc tgatcgataa gcgatcggac gaccatctcg gctccagaca acatctccat
    36241 ggcctgcctc actgtttgac ggaaaaaatg tgtaaaacac attaaccgtc cgatcttgct
    36301 taagcaattg gcagcctgag acataaacac ggggtaataa gaataaacag gaggaaaggt
    36361 gaaaagaaac gataaaatcc tccattacag aggattaaat tgataataaa tcggctgaat
    36421 cccacaactt acagcaattt ctctattttt ttaattaatc ctaaaatagc gagatcacac
    36481 attcagaata aaattctata ttcaataaaa aatgcagaat aaaccagaca ttaatgtctg
    36541 acctattctg cattcagtta gcgtttgcaa attgagacta attgctcttc catccactga
    36601 tggcctttat cgcgcccggc agcttcatgc caggagagat aacaggttct gctgttttgt
    36661 tttaacggca gcggtaatac ctgtaactct aaggattcag cgaactcttc agccagccaa
    36721 cgcggcgcaa tagcgaccag atgcgtttgc gacaccacgc taagtacgct catcattgcc
    36781 atgccctgat acgcgatact ggcttgctta tctaccgtgt cataccaggt ttgactaaat
    36841 gacgcgaaac gatcgagcga aaccgcagca tgttgttcgt tataaacatc atgtttcagt
    36901 aacgggcctt taattgttgg atgatttttg ctggctacca acaccatttc atctttaaat
    36961 aatggcacac tggtaaattc agggcgatgg aagtcttcat aactaatcac aaactccgtt
    37021 tcctgataac gcagctgatg ttcagtgttc tgatttaatg aagacttgaa cataacatgt
    37081 atatttggcg caatctgctc aatgtgatta taaatctgcg aggtcagaat gctgtctaac
    37141 gggctgcaaa cacaaagatg aaatacacgt tcactgctcg cgggttcaaa acctgaacca
    37201 ggcaattcat tttgtactag ttgcaatgcc tgacgaactg agccaaaaag ttgaaatgcg
    37261 cgagcagtcg gttgaatacc acggccataa cgaacaaaaa gctcgtcatt aaacatcacc
    37321 ttcaggcgtg caacagcgtt actgaccgca ggttgcgaca ttcccagaac atgagcggca
    37381 cgggtgatgt tttgctcctg catcacggca tcgaaaacgg ttaataagtt gagatcgacc
    37441 atgcgtagct gtggcttgct taactccgcc gtctctggat gatctgtttg aacctctggc
    37501 atacttaact ccactgtcac acttaactcc ctttccctta ttggaatgca gagaaataat
    37561 c
//
LOCUS       DAZGHF010000030        37346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGHF010000030 DAZGHF010000000
VERSION     DAZGHF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.030"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            51..473
                     /locus_tag="ACHK3A_001669"
     CDS             51..473
                     /locus_tag="ACHK3A_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739761.1"
                     /translation="MIPSQISFNTLPVNATYASEPTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSTISKFQHQNPVNGSVNPDLLYEDYTD"
     gene            1021..1470
                     /gene="glmS"
                     /locus_tag="ACHK3A_001670"
     CDS             1021..1470
                     /gene="glmS"
                     /locus_tag="ACHK3A_001670"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO8739762.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            1470..2858
                     /gene="glmL"
                     /locus_tag="ACHK3A_001671"
     CDS             1470..2858
                     /gene="glmL"
                     /locus_tag="ACHK3A_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO8739763.1"
                     /translation="MQIVSVDIGSTWTKAALFAREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLDQVLNVDDALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQSYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     VVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSASANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            2877..3362
                     /locus_tag="ACHK3A_001672"
                     /pseudo
     CDS             2877..3362
                     /locus_tag="ACHK3A_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="methylaspartate mutase subunit E; catalyzes the
                     formation of threo-beta-methyl-L-aspartate from
                     L-glutamate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
     gene            3752..5149
                     /locus_tag="ACHK3A_001673"
     CDS             3752..5149
                     /locus_tag="ACHK3A_001673"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFO8739764.1"
                     /translation="MHHLSRLDKAFLRKGIQLSRANNIVSLSRPSGASARVLLPDELP
                     LEEKAVTQLLDFAEVKLPDHPGYVRQTCATPDFHPGNGVPVGAVVASTPDLVIPAAIG
                     TDISCGMRLLTTGLTLEQAEAHKDVIAQRLTQVLLQNERDVPLSAQAFTALFDGGPAD
                     CVSTLPSEGLWIRTNIPRMVQEIDRCIGLNEHNSHSRYAPPALQATDRALLRDPCLGT
                     PGSGNHFIELQVVDSVVDRHVAWQQGLKTGDIVVMIHSGSRDVGFYVGRHWMDRARAE
                     WPTGHKHPQSGLYGLAGGLAEEYLQAIGMAARYAWLNRVTIAEMVRVCLANLFHQDNS
                     HLVVDVPHNIILREHEMNIHRKGATPARVGNLALIPGSMGDYSWLAVGCGNPEWLWSC
                     SHGAGRSQRRQAMRSRATEESTLPWQCVTLREERRFEEAPAAYKDIGPVIEAQQEAGL
                     IQPAVRFRPWLTFKG"
     gene            complement(5294..7927)
                     /gene="mngB"
                     /locus_tag="ACHK3A_001674"
     CDS             complement(5294..7927)
                     /gene="mngB"
                     /locus_tag="ACHK3A_001674"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO8739765.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLTFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKLWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEITHEAWQSRAVIR
                     YDMAVPRNLHERSARQSTGRVGVELAVTLSDNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGLAVF
                     SEGLREYEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGSFSC
                     RLSLFSYTGTPSTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVVLRLFNPAESTTCEATVAFSREVISCTQTMMDESISTAERD
                     NLKELGAFLPGQSRTFSYRLG"
     gene            complement(7945..9861)
                     /gene="mngA"
                     /locus_tag="ACHK3A_001675"
     CDS             complement(7945..9861)
                     /gene="mngA"
                     /locus_tag="ACHK3A_001675"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO8739766.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALNLKRSDETRTVQQDT
                     QPVKSLKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            10031..10753
                     /gene="mngR"
                     /locus_tag="ACHK3A_001676"
     CDS             10031..10753
                     /gene="mngR"
                     /locus_tag="ACHK3A_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO8739767.1"
                     /translation="MGRKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLTDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(10857..11726)
                     /gene="sucD"
                     /locus_tag="ACHK3A_001677"
     CDS             complement(10857..11726)
                     /gene="sucD"
                     /locus_tag="ACHK3A_001677"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO8739768.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11726..12892)
                     /gene="sucC"
                     /locus_tag="ACHK3A_001678"
     CDS             complement(11726..12892)
                     /gene="sucC"
                     /locus_tag="ACHK3A_001678"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO8739769.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(12986..14203)
                     /gene="odhB"
                     /locus_tag="ACHK3A_001679"
     CDS             complement(12986..14203)
                     /gene="odhB"
                     /locus_tag="ACHK3A_001679"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO8739770.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPVKESAP
                     AAPAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(14218..17019)
                     /gene="sucA"
                     /locus_tag="ACHK3A_001680"
     CDS             complement(14218..17019)
                     /gene="sucA"
                     /locus_tag="ACHK3A_001680"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO8739771.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(17320..18036)
                     /gene="sdhB"
                     /locus_tag="ACHK3A_001681"
     CDS             complement(17320..18036)
                     /gene="sdhB"
                     /locus_tag="ACHK3A_001681"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO8739772.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(18052..19818)
                     /gene="sdhA"
                     /locus_tag="ACHK3A_001682"
     CDS             complement(18052..19818)
                     /gene="sdhA"
                     /locus_tag="ACHK3A_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO8739773.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASASDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(19818..20165)
                     /gene="sdhD"
                     /locus_tag="ACHK3A_001683"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(19818..20165)
                     /gene="sdhD"
                     /locus_tag="ACHK3A_001683"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO8739774.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(20159..20563)
                     /gene="sdhC"
                     /locus_tag="ACHK3A_001684"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(20159..20563)
                     /gene="sdhC"
                     /locus_tag="ACHK3A_001684"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO8739775.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            20944..21051
                     /locus_tag="ACHK3A_001685"
     CDS             20944..21051
                     /locus_tag="ACHK3A_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739776.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            21257..22540
                     /gene="gltA"
                     /locus_tag="ACHK3A_001686"
     CDS             21257..22540
                     /gene="gltA"
                     /locus_tag="ACHK3A_001686"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO8739777.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            22930..23496
                     /locus_tag="ACHK3A_001687"
     CDS             22930..23496
                     /locus_tag="ACHK3A_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8739778.1"
                     /translation="MFKGQKTLAALAISLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            23547..26006
                     /gene="ybgQ"
                     /locus_tag="ACHK3A_001688"
     CDS             23547..26006
                     /gene="ybgQ"
                     /locus_tag="ACHK3A_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8739779.1"
                     /translation="MDTVNIYRLSFISCLVMAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKGKGVIVPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRANNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATDSTISLA
                     AYRFSDRHFHSYANYLDHKYNNSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDNSGISVGMVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSLHLPEHMEDTANRLILPCH"
     gene            26021..26752
                     /gene="ybgP"
                     /locus_tag="ACHK3A_001689"
     CDS             26021..26752
                     /gene="ybgP"
                     /locus_tag="ACHK3A_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8739780.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPSPEKNSGHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVITNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAKK"
     gene            26749..27810
                     /gene="ybgO"
                     /locus_tag="ACHK3A_001690"
     CDS             26749..27810
                     /gene="ybgO"
                     /locus_tag="ACHK3A_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8739781.1"
                     /translation="MSAGKGVLLVICLVFLPLKSALALNCYFGTSGGTVEKSEAIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREIPPHDYQSSLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLIEEDKALLIGNGLKLRLLDENSSPYTFNKYSEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            27963..29009
                     /gene="abrB"
                     /locus_tag="ACHK3A_001691"
     CDS             27963..29009
                     /gene="abrB"
                     /locus_tag="ACHK3A_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO8739782.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATNPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(29006..29797)
                     /gene="nei"
                     /locus_tag="ACHK3A_001692"
     CDS             complement(29006..29797)
                     /gene="nei"
                     /locus_tag="ACHK3A_001692"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO8739783.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKPYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTMRVLRVKLQTVDKTIL
                     LYSASDIEMLTPEQLITHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(29833..30567)
                     /gene="pxpA"
                     /locus_tag="ACHK3A_001693"
     CDS             complement(29833..30567)
                     /gene="pxpA"
                     /locus_tag="ACHK3A_001693"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO8739784.1"
                     /translation="MKIDLNADLGEGYASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSVMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(30557..31489)
                     /gene="pxpC"
                     /locus_tag="ACHK3A_001694"
     CDS             complement(30557..31489)
                     /gene="pxpC"
                     /locus_tag="ACHK3A_001694"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO8739785.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(31483..32139)
                     /gene="pxpB"
                     /locus_tag="ACHK3A_001695"
     CDS             complement(31483..32139)
                     /gene="pxpB"
                     /locus_tag="ACHK3A_001695"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO8739786.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGIYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(32162..32905)
                     /gene="ybgI"
                     /locus_tag="ACHK3A_001696"
     CDS             complement(32162..32905)
                     /gene="ybgI"
                     /locus_tag="ACHK3A_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO8739787.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            33176..34657
                     /gene="dtpD"
                     /locus_tag="ACHK3A_001697"
     CDS             33176..34657
                     /gene="dtpD"
                     /locus_tag="ACHK3A_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO8739788.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFSHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDIFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(34700..36118)
                     /gene="phrB"
                     /locus_tag="ACHK3A_001698"
     CDS             complement(34700..36118)
                     /gene="phrB"
                     /locus_tag="ACHK3A_001698"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO8739789.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSASVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLQIALAEKGIPLLFHEVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVQAERALRNVVCEGFDDGVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIDPAPSITLNYPRQPFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSIWLNEL
                     IWREFYRHLITYYPSLCKHRPFIVWTDRVQWQSNSAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(36115..36624)
                     /gene="ybgA"
                     /locus_tag="ACHK3A_001699"
     CDS             complement(36115..36624)
                     /gene="ybgA"
                     /locus_tag="ACHK3A_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO8739790.1"
                     /translation="MNQQRFDESTLIRIFALYELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRGGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(36766..37346)
                     /locus_tag="ACHK3A_001700"
                     /pseudo
     CDS             complement(36766..37346)
                     /locus_tag="ACHK3A_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         9127 a         9926 c         9104 g         9189 t
ORIGIN      
        1 taaacaatgc ccggggatta atttttatta ctcacggaaa gatttaaata atgatcccat
       61 ctcaaatctc cttcaacacc cttccagtca atgcaacata cgcttcagag ccaacagttg
      121 acacacaaaa attctctgac atcctctact cagcggggtg ttccctaaag gatctggctt
      181 atctgagcag atgtctggca tcaatacatc cgactatcgc caaaaacatt tatgaaacag
      241 aaaatctcag cgatcaaaaa ttactacata ttgattgccg tagtacaaat gaaatcaaga
      301 taaatatctt ttttggtcaa caacgagaag gattaattga aattaattct gacacagtca
      361 ttttttcgat actgtcttca ttgattgact ccaccatttc taaatttcaa caccaaaacc
      421 ccgttaacgg ttctgtaaat cctgacctat tgtatgagga ttatactgat taaatccctc
      481 ctgtcgcgaa aaatggcctg cgttattgcc agacgtaata aaacttttga ttaacgtttc
      541 tcgtactggt agcaccttca tgtacttttc ttttttacga aaaatcaaaa gagctaacaa
      601 aggtcataat tcgcatgaat cattaattat ttgttatttt caacattatt tttacattaa
      661 gcagagatga attgcagtga aattcgactt aacttttatg aaatgcattt actcatggct
      721 aaccccatat cactaaaatg caaattagaa gttgatcaca attatctcaa tacatcaaat
      781 caagttaatt ttaaaaattt aacattcatg acattagttg acataacaca ctgaattaac
      841 atgagaattt gaaaacacac tttaaacaat catttcccga aagacatttt gaaaatagat
      901 caattgcaac attacggtca gataaagaca ctcctgtgat attaaacctg tgccgttatg
      961 agcggccata aatacgacgt gtgtaactta ttttactctc tgtcttacaa aggattaccc
     1021 atgaagaaag caacacttgt gattggcgtt attggcgcgg actgccatgc agtaggcaat
     1081 aaagttctgg accgcgtttt tagcaaccat gacttcagag ttattaatct gggcgtaatg
     1141 gtcagccagg acgaatatat tgatgctgcc attgaaactg gtgctgacgc gattgtcgtc
     1201 tcctctatct atgggcatgg cgatatcgac tgtctgggca tgcgcgaacg ctgcatcgag
     1261 cgtggactgg gggacatcct gctctatgtg ggcggtaacc tggtggtcgg aaaacatgac
     1321 ttcgctgacg tggaaaccaa attcaaagag atgggatttg atcgcgtctt tgccccaagt
     1381 catgacttag aagatgtttg tcagctgatg gcccacgata tcaaccaacg tcatgacgtt
     1441 gatactcgta ttcttgaaga ggcaatctga tgcaaatcgt ctctgtcgat attggatcga
     1501 cgtggaccaa agcagccctc ttcgcccggg agggggacgc gttaacgttg gttaaccacg
     1561 tactgacccc aaccaccacc catcatctgg cgaaggggtt tttctccagc ctggaccagg
     1621 tgttaaacgt agatgatgcc ctccccctgt taaacagtgg tgaggttgca ttgaagtatt
     1681 cctcctctgc caaaggtggg ctggcggttg ccgcaatggg attggtgcct tctatcaccc
     1741 tggaaaccgc gaaagtaacc gctcactctg cgggtgctaa aattgcgcaa tcttacgcct
     1801 acaagctgaa ccgccgtgat atccaggcac tggaagaaac gcagcccgat attctcttgt
     1861 tcaccggcgg caccgatggc ggagaagaaa gctacggact gaataacgcc cgtgtgttgg
     1921 cagaatccaa actcgactgc gccattattt atgccggaaa ccgggatatt caggacgagg
     1981 tgcaggagat cctcgggcat aaagacctga ccatcgtcga caacgttctg cccgatcttg
     2041 accatccgaa ccctttagct gcacgccagg ctatctgcga catattcctt aaacgcatcg
     2101 ttaaagggaa agggctggat gtcgttgttg ataaaacagg cgaagaacca atgccgacac
     2161 cgtggacagt ctttgaactg gttaaggcaa tcagtaatgt cgatcacagc tggaaagagt
     2221 tcatgctgat cgacatgggt ggcgcaacca ccgatgtcta ttccgccagt gccaacacgc
     2281 tctcgcccga cactgtttta catggcgtac cggaaccctt cgtaaaacgg acggtggaag
     2341 gagatctggg aatgcgcgtc tcggcaatgg tggttggtga aagtgcgaaa gagttagtca
     2401 atgtgaattt tgctcagcaa cccgcacagc tggacgcttt ttatcattac ctccgtcatc
     2461 tagtcgctca tcccgactac ttacccgcga atgcagaaga aaaatatttc gataccgtcc
     2521 tggccggact gtgtgtgggt tatgccaccg aaagacatgc gggcaccaaa aaagaggtct
     2581 gcacctgcgt cggcaatgtc gatttacaga tgggacgtga cctgacaacc gtgcgtaaag
     2641 tcgtcggatc cggcggatgg ctctcgcgtg ccagtcagtt cgatatgcat cactggttga
     2701 aataccgcga gttaaacgac gacggtaaac gcattctctt accgaccgaa tttgaatatt
     2761 accgcgattc ccgggggcta ttaccactgc tggcaaacgt cgccagagtc gatccgctgg
     2821 ccgccgcgcg caccagtatt caatgcttaa ccctataaca attaaggctg caatcaatgg
     2881 aacttcgaaa taagaaatta acccatgacg aattcatgac cgagcggcaa caggtactga
     2941 aaacctggga aaccggcaag gatgttgaaa actttgaaga tggcgttaaa taccaacaaa
     3001 ccattcctga gcataaacgc ttctctcttg cgctgttgaa agcagacaag gaaggtaaaa
     3061 cccttagcca accgcgcgca ggtgtggcgc tgatggacga acacatcgaa ctactgaaaa
     3121 cactacaaga agagtgcgat ctgctgccga gcaccatcga tgcatatacc cgtctgaatc
     3181 ggtacgaaga agcggctgta gggattaaaa aatccatcga agccggaacc tctaaactga
     3241 acggtttacc ggtcgttaac cacggcgttg cagcttgccg tcgcctcacc gaaactctgc
     3301 aaaaaccgct acagatccgc cacggcacgc cagatgcgcg tctgctggct gaaatctcga
     3361 tgtgtataca tgaatatact ctaaataagt caagttgcag gaagggaaca aataatgaat
     3421 ttccagaagc ttacataagt aagtgacctg ggtgagcgaa catcgatgca gcacatgcaa
     3481 cttgaattat gacaagtata caatgataaa tttagatacc ctatgttata tattatctat
     3541 gtccggttaa attgaatgag tcatggcctt aaaagacttc acaaaagata attcctcttt
     3601 aacaataagc cagatttacc acttgtaaca cttgctatcg ccacatacac tacgcgcgtt
     3661 ttctttccct tttgctggct gctgaccgga aagacaaaat taccctcgcc atacaggggg
     3721 ttgtttaacg tctatatttc aggacagcgt tatgcatcat ctttcaaggc ttgataaagc
     3781 ctttctgcgc aaagggatcc aactttcccg cgccaacaat attgtttcat tatctcgtcc
     3841 atcaggtgcc agtgcccgcg tactgctgcc ggacgaactg ccgctggaag aaaaagcagt
     3901 aacacaactg cttgattttg cagaagtgaa gctaccagac cacccaggtt atgtgcgcca
     3961 gacgtgcgcg acgccagatt ttcatcccgg caatggcgtc cccgttggcg cggtggtggc
     4021 ctcaacgccg gatttagtca ttcccgccgc catcggcacc gatatttctt gtggcatgcg
     4081 gctgctgacg accggattaa cacttgagca agccgaagcg cataaagacg tcatcgctca
     4141 gcggttaacg caagtgctac tgcaaaatga gcgcgatgtt ccgttatccg cacaggcgtt
     4201 caccgcgctt tttgatggcg gaccggcaga ttgcgtgtcg accttgccat cagaaggtct
     4261 ctggatacgc acgaatatcc cccgaatggt acaagagatt gaccgctgca ttgggcttaa
     4321 cgagcataac agtcattcgc gttatgcccc acccgccctg caggcaacgg accgcgcatt
     4381 actccgcgat ccttgtctgg gtacgccggg ttcaggtaat cattttattg aacttcaggt
     4441 cgttgatagc gtggttgatc gccatgtagc ctggcaacaa gggctgaaaa caggcgatat
     4501 tgtggtgatg atccacagtg gatcgcgaga tgttggcttt tacgtcggaa gacactggat
     4561 ggatcgcgcc cgtgctgaat ggcccaccgg acataaacat ccacaaagcg ggttatatgg
     4621 tctggcaggc ggactggcgg aggaatatct acaagcgata ggaatggcgg cgcgttacgc
     4681 atggttaaat cgcgtcacaa ttgccgaaat ggtgcgcgta tgtcttgcca atctctttca
     4741 tcaggacaat tcgcatctgg tggtggatgt gccacataac atcatccttc gcgagcatga
     4801 aatgaatatt catcgcaaag gcgcaacacc ggcacgagtg ggcaatctgg cgttaatccc
     4861 cggttcaatg ggcgattatt cgtggcttgc ggtaggttgc ggcaacccgg agtggctgtg
     4921 gtcatgcagt cacggagcag gacgcagcca gcgtcggcag gccatgcgct cacgcgcgac
     4981 tgaagagtca acgctgccgt ggcaatgcgt cacattgcgc gaagaacgac gttttgaaga
     5041 agcgccagcg gcttataaag atattggtcc ggttatcgaa gcccaacaag aagccggatt
     5101 aattcagcca gcggtcagat ttcgaccgtg gttaacgttt aaggggtaac tgaaatgata
     5161 aatggcctgc ggttctcacg caggtcaaat ctggtatctg cgtaagctaa caaacttgtt
     5221 tatcaacaat ctcaggccat ccgaagatgg cctgaaatta ttgcccccct cagatacact
     5281 tttagcgcaa taatcagcca aggcggtagc taaatgtccg tgactgcccc ggtaaaaatg
     5341 cccccagttc ctttaaatta tctctttccg ctgtgctgat gctttcatcc atcatcgttt
     5401 gcgtacatga aatcacctca cgactgaacg cgacagttgc ctcacaggtt gtcgattctg
     5461 ccggattaaa cagtcgcaat accacttctt gccggtcttc cgctttttta agagcgctaa
     5521 tcaggcatcc cacagggggc attttcagca agctatagct ttccggcaca ttgaatccgg
     5581 ctttatttaa cttcatcgca tcccacggga ttttgttgta acactgtact ggcgtcagcc
     5641 atgcgcgcgc ctgctgggct acaccggcgg tcgaaggcgt acctgtataa ctaaataagc
     5701 tcagacggca gctaaaggag ccacgtaatt gtgaatcggg caccggcatt tttattcctg
     5761 aaggacgtcc aggccttaaa agcagatctt ctttccccag taagcccacg ccacgcagca
     5821 acgtaatggc aaaggttttc ttctcttcac cgatgacttc atattcacgt agcccttcgc
     5881 taaagacagc cagaccgtta cgcccttcct gtaaggcagc atagttgagc atattccata
     5941 ccggaaccgg cgcttctttc cagccttctt gctgccagtt gttcattgca ctgtcgttca
     6001 cggggcgcgt tagcgaacca aactgggtat cggccagaac actgtcggtg ttaaaaggtg
     6061 tagggaccag gacgcgaagg cgatgatcgt cagcctggtt atcaaggttg atatccacat
     6121 caatgcgcct gctgttatca ctcagagtga cagccaactc cacccctact ctgccagtgg
     6181 attgtcttgc gctgcgttca tgcagattac gcggcaccgc catgtcatag cggataacag
     6241 ccctactctg ccaggcctca tgagtaatct cgcattgcgg tttcgcgttc gccgaagtaa
     6301 ttacccactc ttcttttgcg ggtgaatagt catattcatc accatcatca gagctttctt
     6361 caatttgcaa cacccggtca tagcgcacac cgctgtcttt atctaccagt tgtagcgttc
     6421 catcctcatt gagcgcaatt tgccagaaag cattttccag tatcccttca gcgtcacttt
     6481 ttgccgcaat cacgttgcca ggctgattcg cttcgatata aagcgtgcga tagcccatag
     6541 aagggacaat ctggttgatc tgaatatcaa actccataaa gggatcataa ttaccgtaat
     6601 gaacgatttg ccgatcgatt aggccaggat cgatctcacg ggcatggcga ataaaatacg
     6661 gtacaggctg accgcgatcg tcccgcaaat taaactggct ggcgcgcagc cgcacagtgg
     6721 tgttgatcac ttcttcccgc ggccagggca tcaggttaaa cagcacaagt ttgtcggcat
     6781 cactctgtgg catgttatcg gcaattttac gcatatagaa acgcagcaga ttatccgcca
     6841 tgtcttctgc cagttcgaaa cgggcgacaa tctcgcgatg aactttgtca ctacagcagc
     6901 agccgatgct gtcatgggcg tggtttttta aaatttcttt ccacaatttc tccagcaagc
     6961 cgtggtggta ttcaaaaccc aacgtccagg ccagcgttgc cagcggttcc agcagattaa
     7021 caatcttatt ttcaatacgc gcgtgggcaa ttttgatatc catacgcgta gaaccgatgg
     7081 tgcgatgcac gcgcatatat ttgccatcaa taaattcccc tttcagggtt gccagattat
     7141 ctcgctgcgc ttcgatcttc tcaaatacct cttcaaagcg gctcatcaca aacttacgtt
     7201 gagggtagat ctcacgtagc ttatccatca cttcgaagat attttgctgc aatggcatct
     7261 ggtcatgacc attcggcagc aaaatctctt tggttactga cgctttttcc agcacatcaa
     7321 aataactgtc gaggcgtttg cgtaatccgt cttcgtcggc aggtaagtac ttaccgatgg
     7381 cgtagcccag cggcagcacc tgcgccgtca cttcgctacc gtcactgctt tgccacaaaa
     7441 actcggtttt atcagtaccg tggcgctcag aacatccgcg ccagaacatg gtgcgggtaa
     7501 tgccaaatcc attgtaaata tgcggcagtt gcccggacat gccaaaggaa tctggtaaat
     7561 aacctatttt catcggctcg ccaaacgtga ggcagtcacg cattccgtac atcagattac
     7621 ggacgatgga ttccgcagaa acaatcgtgg tatcggtctg ggtataccag gggccgataa
     7681 tcaacttgcc tgcttctacc tgtttcttca cacggtcttt gttttccggt ttcaccgcga
     7741 aataatcttc gaggatcgcc gtttgcccgt cgagtacgta atatttgtat tcgttgtcct
     7801 gttccagtcg gcacaggatc tcttccatat tattgaccag cagaatgcgt gactcttcgg
     7861 tggtgaaata ccactcccga tcccagtgca tatgcggggt gatgtgaacg cgagataccg
     7921 ctttcatcgt cgtttccttt ttgtttatgg cattacgcca tcagtcagat agttgccatg
     7981 cttaaccgcg tgacgccgcc agatcagaag aattgcagtc gagattgcag cccccaccaa
     8041 tgccgcacca aaccagccaa ttgccgccat aacaccgccc gcgccattat catgaagtaa
     8101 aaagagcgag aaaatgccgg caccgggtgt cgaaaggccg atattcatcg cgccgacgat
     8161 agcgcccgtt accatagagc ccagcacaaa cgaaccaata acccgcagcg gatcttcaat
     8221 cgccatcggg atcgcccctt cggtaatacc tgccagccct aacagccagg tggatttccc
     8281 ggtctcaatt tcaaactctt taaacaggcg cggtgcgagc atcgtggaag cggttacggt
     8341 aaatgccgaa accattttga cggaggcgaa aatggcatac gggccgtaaa cgccgttcgc
     8401 cattgcgccc aggcagaatg cataagcggc tttgttcact ggccctccaa ggtcaaagga
     8461 acacataaaa ccgagaatgg cacccagcaa cagcgcgtta cttcctgaca gaccgttcag
     8521 ccaggcggta agcgagttat tgatccaggc gacaggttcc cccaccacaa acagcatcag
     8581 actgcccgct cccaacgtac cgagcaccgg gtagagataa aaagtcagga atccattgaa
     8641 tttactgcta agacgcaagt gatttttcac ccagcgcatc agataaccgg ctatcaatcc
     8701 gccaacgaca gcaccgagaa acccggaacc gatcatgttg gcggcaagtc cagccgcaaa
     8761 gcctggcgct aacgccggtt tatcagccag tgaataggcg gtgtaggctg ccagcaccgg
     8821 taccatcaaa atcccgagca gccctccgcc cagtttgcgg tacatccaca gccaggagtt
     8881 ttcttcatta aacaaatctt gtagcccgaa gatttgcgac agtaataccg caaccgccag
     8941 caccgtgccc cccgcgacaa tcaacggcac tgcaaaagag atcccgctca acagtgcctg
     9001 ttttagctcc gttttgaggc ttttcaccgg ttgcgtatct tgctgtacgg tacgcgtctc
     9061 atcgctacgc ttgaggttaa gcgcttgttg gatcaacgct tctgcatggc gaatcggctc
     9121 ggcaacaggc acagaaagcg cgggaatgcc gttaaaacgc tcactctcct tgatggcgac
     9181 ttcagccgca aaaatacagg cggttgcact attgagttgc tctgccgtta aacgcccttc
     9241 aatgccgtta gcaccttgct tttcaacgta gacattcacg ccgagtttgc gtccggcttt
     9301 ttccagatac tccgcagcca tataggtgtg agcaataccc gccgggcagg ccgttacgca
     9361 gacgatagtt ggcgcgttgg aaaaagaagc tgagggttgc gtgcctccct tgtcatccag
     9421 cgccgagagc aattcatcag gcgtcgtcgc cgactgtata cgcgcccgta tctcatcatc
     9481 cgccaggcgc gtggtcagcg ctgtcagcag ttgcatgtgc gtagtacccg cttcattggg
     9541 gggaatcgcc agcaacacca ctaaatcaac cgcttccggg ccatccacgc cttcccactg
     9601 aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct tttaccgcag cagttttgcc
     9661 atgcggcaca gccagccctt cacctaaggc ggtcgggcca aggctttcac ggcgatacac
     9721 ttctttcaga aattgctcag tactggaaat tttccccaga gcagcaagac gttgagtcaa
     9781 cgcgtggatg gcctcttcac ggctggtaaa gcgcgcattc agacacaacg catcgcggtg
     9841 ggtcagagtc gtcaggttca tcatgatcct cacttgatcg tttttatagt cgcgccagtg
     9901 tgcccgataa aacaatacca ttatgtgatc aatttcactt gattgaaatt atttgtattg
     9961 tatttgtatt atttacagga tgaatttatc gggcataatg gctaaattcg tattaatgag
    10021 atgctaaatc atgggacgga agcccttata ccgacagatt gctgatcgca ttcgtgagca
    10081 aattgcgcgt ggcgagctta aacctggcga tgcgttaccc actgaatcag cgctacaaac
    10141 agagtttggc gtcagccggg ttacggtgcg tcaggctttg cgccagttag ttgaacagca
    10201 gatcctcgaa agtattcagg gcagcgggac ttacgtaaaa gaagagcggg tcaattacga
    10261 tatttttcag ttaaccagtt ttgatgaaaa actgacggac cgtcacgtcg atacacacag
    10321 tgaagttctg atattcgaag tgattccggc tgacgatttt cttcagcaac agctacaaat
    10381 caccgcgcag gatcgtgtgt ggcatgtgaa gcgcgtgcgc tatcgcaagc aaaagccaat
    10441 ggcgctggaa gaaacctgga tgccgcttgc gttgttcccg gatctcacct ggcaggtcat
    10501 ggaaaattcg aaatatcact ttattgaaga agtgaagaaa atggttatcg atcgtagcga
    10561 acaggaaatc attcctctga tgccaaccga agaaatgagc cgtctgctta atatcagcca
    10621 gactaagccg attcttgaaa aagtatcgcg tggatattta gttgatggcc gggtgtttga
    10681 atatagccgc aacgctttta ataccgatga ctataaattt accctgatag cgcagcgaaa
    10741 atcatcgcga taagcataaa aaaggccatc atacgatggc caaccatgtc gaaaccgaca
    10801 gcccttcgcc attcctgtag aaggaagcgg cgagggctat ttcttattac agatatttat
    10861 ttcagaacag ttttcagtgc ttcaccgata tccgccaggc tgcgaacggt tttcacgcct
    10921 gcggcttcca gagcagcgaa tttctcatcc gcagtccctt tcccaccggc aatgatcgca
    10981 cccgcgtggc ccatacgttt gcctttcggc gcagtcacac cagcgatgta acctacaact
    11041 ggcttggtaa cgtgctcttt gatgtacgcc gctgcttctt cttcagcgct accgccgatc
    11101 tcaccgatca tcacgatcgc ttcggtctgc ggatcttttt cgaacatttc gaggatgtcg
    11161 ataaagttag agccagggat cggatcgccg ccgataccaa cacaggtcga ctgaccgaaa
    11221 ccgtaatccg tggtctgttt aaccgcttca taggtcagcg taccggaacg ggaaacgata
    11281 cccactttac ccggtttgtg aatgtgacca ggctggatac cgattttgca ttcacccgga
    11341 gtgataacgc ctgggcagtt cgggccgatc atacgaacgc ctgcttcatc cagcttcact
    11401 ttcacggtca gcatatccag cgtcgggatg ccttcagtga tggtgataat cagtttgatg
    11461 cctgcatcga tagcttccag aatggagtct ttgcagaacg gtgctggtac gtagataaca
    11521 gaagcggtag cgccagtagc agcaacagct tcacgcacgg tgttgaacac cggcaggccg
    11581 aggtgggtgg tgccgccttt acctggggtt acgccgccaa ccattttagt gccgtatgca
    11641 atggcttgtt cagagtggaa agtcccctgg ctaccggtaa agccctggca gataaccttg
    11701 gtgtttttat caattaaaat ggacattatt tcccctccac tgcggcaaca acctgctgag
    11761 ctgcatccgt cagacctttt gctgcaataa tattcaggcc gctgtcagcc agtttcttcg
    11821 cgccgagttc ggcgttgtta ccttccagac gtaccacgac cggtacgtta acacccactt
    11881 ccgctaccgc gccgatgata ccgtcagcga tcaggtcgca acgaacgata ccgccgaaga
    11941 tgttaaccag aacggctttc actttgtcgt cagagaggat gattttgaac gcttcggtta
    12001 cacgttcttt ggttgcgccg ccgccaacgt ccaggaagtt agctggttcg ccgccgtgca
    12061 gtttaacgat gtccatggtg cccatcgcca ggcctgcgcc gttaaccata caaccgatgt
    12121 taccgtccag cgcaacgtag ttcagttccc actgtgcagc ctgtgcttca cgcggatctt
    12181 cctgcgactg gtcacgcatt tcgcgcagat caggctggcg gaacagtgcg ttgccatcag
    12241 cgcccagttt gccgtcgagg caaatcagat cgccctgttt ggtaatgacc agcgggttga
    12301 tttcgatcag cgccaggtcg cgctccagga aaatggtcgc caggcccatg aagattttgg
    12361 tgaactgctg aaccagttta ccttccagac ccagtttgaa cgccagctcg cgtccctgat
    12421 acggcatcgg gccagtcagc ggatcaagcg caactttgtg gatcaggtgc ggagtttctt
    12481 ccgccacttt ttcgatttcc acgccgcctt cggtggaggc cataaagacc acacgacggg
    12541 aactacggtc aacaacggca ccgagataca gctctttagc gatatcggtc gctgcttcaa
    12601 ccagaatctg gttaaccggt tggccattgg catctgtttg atacgttacc agacgcttgc
    12661 ccagccagtt ttctgcaaaa gcacggatgt cttctttgct gtttacaact ttcacaccgc
    12721 ccgctttacc gcggccacca gcgtgaacct gacatttcac tacccacgga ccggcaccga
    12781 tttttgaagc ggcttcttct gcttcgcgcg gagtagtaca ggcataaccc accggtgctg
    12841 gtaagccata gcgggcaaaa agttgttttg cctgatattc atgtaagttc atgtgttctg
    12901 tccatccttc agtaatcgtt atcttttaaa ccgtagaccg acaccctatc cacgtcggtc
    12961 tacagtgcag gtgaagctta aactactaca cgtccagcag cagacgcgtc ggatcttcca
    13021 gcaactcttt gatcgttacc aggaagccca cggattcgcg accatcgatc agacggtgat
    13081 cgtaggacag cgccaggtac atcatcggca ggatctcaac ctgaccattc accgccatcg
    13141 gacgatcttt gatagcgtgc atacccagaa ttgcgctctg cggtgggttg atgataggcg
    13201 ttgacatcag ggaaccgaac acaccaccgt tggtgatggt gaagttacca ccagtcagat
    13261 cttcaaccgt cagcttgccg tcacggcctt taacagccag ctctttgatt ttcttctcga
    13321 tgtctgccat gccgagggta tcgacatcac gcagaaccgg cgtcaccagg ccgcgcggcg
    13381 tagaaaccgc catactgacg tcgaaatagt tgtggtagac cacatcatca ccgtcgatag
    13441 acgcattcac ttccgggtaa cgtttcaggg cttcaaccac cgctttcacg tagaaggaca
    13501 taaagcccag acggatgccg tggcgttttt caaacgcttc accgtactgc ttacgcagat
    13561 ccataatcgg cttcatgttg acttcgttga acgtggtcag catggcggtg gagtttttcg
    13621 cttccagcag acgctctgcc acacgcttac gcaggcgagt catcggcaca cgtttttcgc
    13681 tacgtgcagc cagggccggt tgcgccgccg gagcaggcgc tgccggagca gactctttca
    13741 ccggggcttt cgccagatgt ttttccacat cttcacgggt cagacgacca cccacaccgg
    13801 tgcctttaat ggcgctggcg tcgaggttat gttcagccag caggcgacgg atcgccgggc
    13861 ttaacgcatc gttgttttgc tcttccagag acgcctgctg gcgttgcgcc ggagtggacg
    13921 ctttctcttc agatttggcg ctggtttctt tacccgcgct gttgccttca cgcaggcgac
    13981 caaggatctg acgagacgtt accgttgtac cttcatcttc cagaaccgca tccagaatgc
    14041 cgtctgctga tgccggtact tccagtacca ctttgtcagt ttcgatttct accagcactt
    14101 catcacgtac gactgcgtcg ccgggttttt tatgccaggt tgcgacggtg gcatcggcta
    14161 cggattcagg caggtcaggg accagaatat ctacgctact cattgtgtat cctttattta
    14221 ttcgacgttc agcgcgtcat taaccagatc ttgttgctgt ttctggtgaa cggacatata
    14281 ccctaccgcc ggagaggcgg aggccgggcg gcctgcataa cgcagagaag ccccaaacgg
    14341 aatcacttca cggaaatgat gctggctgca gtaccatgcg ccctggttga gcggctcttc
    14401 ctggcaccag acaaaatcct tgacgtgagc aaactgctgc aacacttcct gcatcgcttt
    14461 atgcgggaac gggtagagtt gctcgatacg cacaatggcg acatcgtgtt gattgttctt
    14521 acgacgttgt tccagcaggt cgtaataaac cttaccagaa cacatcacta cgcgcttcac
    14581 gcccttcgga tcaagctcgt cgatttcacc gatggctggc aggaaggtgc cgttcgccag
    14641 ttcttcgagg ctggataccg ccagcggatg acgcagcagg gatttcggcg acatcacgac
    14701 cagtggacga cgcatcccgc gcaacgcctg acgacgcagc atgtggtaaa cctgtgccgg
    14761 ggtagacggt acgcaaacct gcatgttttg ctcagcgcaa agttgcagat aacgttccag
    14821 acgcgcggag gagtgctccg gcccctgccc ttcgtaaccg tgcggcagca acatcaccag
    14881 gccacacatc cggccccatt tctgttcgcc agaggagatg aactggtcga taaccacctg
    14941 cgcaccgttg gcgaagtcac cgaactgcgc ttcccagatg gtcagagtgc gtggttctgc
    15001 ggtggcataa ccgtattcaa acgccagcac ggcttcttct gacagtacgg agtcccagac
    15061 acggaacgcg ccctgaccgt tatggatgtg ttgcagcggc gtgtaagtgg aaccgttaga
    15121 ctggttgtgg atcaccgcgt ggcggtggaa gaaggtaccg cgaccggagt cttcacccga
    15181 caggcgaacc ggaatgcctt catcaaccag cgtggcgtaa gcgaggtttt ccgcaccgcc
    15241 ccagtcgaac agtttctcac cggctgccat tgcctggcga tcgccataaa tcttggcaac
    15301 gcgagactgc atttcaactg cttccggcac cgtgctgatg cgtttagcca gttcctgcag
    15361 gcgcttcatc tcaactttgt tcgggtactc ttcgtcccat tcgtggttga ggtacggcga
    15421 ccaggtgaaa gagtgcatgt tcatcggacg ccactctgct acaacgcaat cgccagcatc
    15481 cagcgcatcg cggtacaggt taaccatctc ggtggcatct tccagcgtcg cgactttttc
    15541 ctgctccagc ttgtcagcat agattttgcg cggcgtcgga tgttttttga ttttctgata
    15601 catcagcggc tgggttgcgc tcggctcgtc ggcttcgttg tggccgtgac ggcggtagca
    15661 caccagatcg atgaagacat cacgtttaaa ggtgttacgg aaatcgagcg ccagacgggt
    15721 cacaaaggca acggcttccg gatcatccgc attaacgtgg aaaatcggtg cctgtaccat
    15781 cttgccgata tcagtacagt acggcgtcga acgggcatcc agcgggttag aggtggtgaa
    15841 gccaacctgg ttgttgataa cgatacgtac cgtaccgcca acttcataac cacgcgcttt
    15901 cgacatgttc agggtttcct gaaccacgcc ctgcccggtc actgcggcgt caccatgaat
    15961 ggtgattggc agcactttgt tgctgctcgg ctcatcaagt ctgtccagac gggcacgaac
    16021 agaaccgata acgaccgggc tgacaatctc aaggtgagac gggttaaacg ccagcgccag
    16081 gtgcaccagg ccgccatcgg tctggaagtc agacgagaag cccatgtggt atttcacgtc
    16141 gcccgtgccg aggtgttctt tatgtttacc ggcaaactcg tcgaacaagt cttgcggttt
    16201 tttacccagc acgttcacca gcacgttcag acgaccacgg tgcgccatcc cgagaaccac
    16261 ttcgcgggtg ccgctgttgc cagcgtggcg gatcatctct ttaagcatcg ggattaacgc
    16321 gtcaccacct tccagcgaga agcgttttgc gccagggaat tttgcgccga ggtaacgttc
    16381 aaggccttca gcggcggtca gttcgcttaa gaagcgtttt ttctcttcgc tattgaaagt
    16441 cgcgcgacca gactcgatac gctgttggat ccagcgtttt tcttcagtgc tggtaatgtg
    16501 catatactcg gcaccaatcg ggccgcagta ggtttgcttg agggcttcca gcagctcgcc
    16561 gagtttcatg gtttctttgc cgctggcaaa tgaaccgacg ttgaaggtct cctggaagtc
    16621 tgcttcggtc agatcgtgga aagacggatc cagatcggcc actttatctt gctgccacag
    16681 tcccagcgga tcgagattcg catgctggtg accacggaag cggtatgcgt taatgagctg
    16741 caggacttta acctgcttca cattggtgtc agggtcggag atcgttgaag agtaacgtga
    16801 agcgtctttc gccaggcggc ggaaatattc acgcgtttga gagtggaatt gatccggttt
    16861 gactcccgta ccaggtaact gctggaacgt cgaacgccag ttagcgtcaa ccgagtcagg
    16921 atcggttaag aagtcttcat agagctgttc tatccagctc tggtttgcgc cagagaggta
    16981 agaagagtcc aaccaggctt tcaaagcgct gttctgcatc gtgatccctt aagcatcttt
    17041 tttatgctta cttcgccgtg gatactacca cgcacagtga tgtgcgtgcc ggggttcact
    17101 tgcgagctct tgtctacgta gtggctcgcg aaggaacctt taaaaactgt cttagtgtca
    17161 ggggcagttt ttaaagattt cctgcatttc tttgttacgc ctgatgcgct gcgcttatca
    17221 ggcctacggg taatgacttg taggccggat aaggcgttca cgccgcatcc ggcactggtt
    17281 gcctgatgcg acgcttgcgc gtcttctcag gcctacggtt tacgcattac gttgcaacaa
    17341 catcgacttg atatggccga tggcgcgcgt cgggttcagc cccttcggac atacactgac
    17401 gcagttcatg atgctgtgac agcggaatac actgaatgca tcgctcaaac cgtcgaggcg
    17461 gctgtcagtc tcggtatcac ggctatcgat caggaaacga tatgccgcta acaagcctgc
    17521 cgggccgata aacttatcgg gattccacca gaaagacgga caagaggttg aacaacatgc
    17581 gcagagaata cattcataca acccgtcgag tttttcgcgc tgctctggca tctgtaaatg
    17641 ctcgcgagct ggcggatttt gtccattatt caacaggtaa ggcttaattt tctcatattg
    17701 cgcatagaat tgtcccatgt ctaccaccaa atcgcggatc accggtaaac ctggcagcgg
    17761 gcgaatcaca atcttcttgc ccggctggtt gagtgccgaa atcggggtaa tacaggccag
    17821 accattcttg ccgttcatgt tcagaccgtc ggaaccacac acaccttcac ggcaggagcg
    17881 acggaacgac aggctgggat ctttctcttt cagctggata agcgcatcca gcagcatcat
    17941 gtcacgacct tcttccgctt ccagggtgta atcctgcata cgcggagcat catcaacatc
    18001 cgggttatag cgataaattg aaaactcgag tctcattttc ctgtctccgc attagtaagt
    18061 acgaatcttc ggcgggaatg ccgggcgcag tttcggttcc atgttgacgc ttcggcgcgt
    18121 catggattcc gactcaggca gatacaggga gtggcacagc cagttttcat catcacgatc
    18181 cgggaagtcg aagcggctat gtgcgccacg gctttcggta cggaagttgg cagaaacagc
    18241 cgttgcatac gccgtttcca tcaggttatc cagttccagg cactcaacgc gctgggtatt
    18301 gaactcactt gaagtgtcat ccagacgggc atttttcaga cgctcgcgga taactttcaa
    18361 ctgctcaagc cctttcgcca tcgcatcacc ttcacggaag accgagaagt tatgctgcat
    18421 acattcttgc agtgctttac ggatcgccac cggatcttca ccgttacggt tattgttcca
    18481 gcggttcagg cgatccagag acgcttctac atcagacgcg ctggcatcgc gcagtgcgcc
    18541 ctgctcggcg atagactctt gcagatgcag acctgccgca cgaccaaata cgaccaggtc
    18601 cagcagcgag ttgccgccca gacggttagc gccatgtacc gatacacaag cgatttcacc
    18661 aacggcaaat agccccggaa caaccacatc ttcgcctttc tcgttcaccg tcagcgcctg
    18721 accggtcact ttggtcggaa taccgcccat catgtagtga caggttggga taaccggaat
    18781 cggctctttc accggatcaa cgtgagcgaa ggtgcgggag agttcaagga tacccggcag
    18841 acgggattca agaacttctt tacccagatg gtcaagtttc agtttcgcgt gtggccccca
    18901 cggaccatca cagccgcgac cttcacggat ttcaatcatg atggaacgcg ccaccacgtc
    18961 acggcccgcc aggtctttgg cgttcggtgc ataacgctcc ataaagcgtt cgccatgttt
    19021 gttcagcaga taaccgcctt caccacggca cccttcggtg accagtacgc ccgcaccggc
    19081 aataccagtc gggtggaact gccacatttc catatcctgc actggtacgc ctgcacggat
    19141 agccatgccg acgccgtcgc cagtgttaat gtgggcgttg gtagtggact gataaatacg
    19201 ccctgccccg ccagtcgcca gcacggtagc gcgagctttg aagtaaacca cttcaccagt
    19261 ttcgatgcac agtgcggtac aaccgaccac cgcgccatcc tggtttttca ccagatccag
    19321 cgcataccac tcggagaaaa tggtggtgtg gtttttcagg ttctgttgat aaagggtgtg
    19381 caacagtgcg tgaccggtac ggtcagctgc cgccgcagta cgtgccgcct gctcgccgcc
    19441 gaagtttttc gactgaccgc caaacggacg ttgatagata cgaccatcat caagacgcga
    19501 gaacggcagg cccatatgtt ccagttccag aatcgcttcc ggcccggttt tacacatata
    19561 ttcaatcgcg tcctggtcac cgatatagtc cgaacctttt acggtgtcgt acatatgcca
    19621 ttcccagtta tcttcatggg tattacccag cgcaacggta atgccgcctt gcgcagaaac
    19681 ggtatgggaa cgggtcggga agactttaga gagcagcgca caggtctgac cgctctggga
    19741 aatttgcagc gccgcgcgca tacctgcgcc accggcacca attacaactg catcaaattc
    19801 tctgactggc aatttcatca cacaccccac accacaacga atccataaat cacgtaaacc
    19861 accagagcaa cgacaatcac cagttgcagc atcaggcgca aggccagcgg tttaacgtag
    19921 tcggtcaaca cctgccacat gccgatccag gcatggatca agatagaaaa cagcgccagc
    19981 agggtgaaca ctttggtgaa cgcagaggcg aagaaaccga tccagacttc atatgtcagc
    20041 tcgccactgg tagcgaaaaa accgaccata taaatgatgt agagcgtcag gacgatagcg
    20101 gtagcgcgaa cgaggatgaa atcatgtacg ccattgcgtc ctaatgcgga ggcgttgctt
    20161 accatacgag gactcctgcg agaagtgaaa gcacgacagt aataacaaag gatattttgg
    20221 cggagcgttt acccgcttcg aatgtttctt ccagatagcc aaaatccatc atcatgtggc
    20281 gaatacctac gacgacgtga tacgccagag cggtaaggat gccccacatg ataaatttga
    20341 cgaagaagct gcccataatc gcggaagctt gctcgaaacc ttcaggggaa gagaggctgg
    20401 tacccagaag ccacagcagg atgcccactg caacaaaggt gatcacaccg gaaacgcgat
    20461 ggagaatgga cgctatcgcc gtgatgggga accggatggt ctgtaggtcc agattaacag
    20521 gtctttgttt tttcacattt cttatcatga ataacgccca catgctgttc ttattattcc
    20581 ctggggacta cgggcacaga ggttaacttt ctgttacctg gagacgtcgg gatttccttc
    20641 ctccggtctg cttgcgggtc agacagcgtc ctttctataa ctgcgcgtca tgcaaaacac
    20701 tgcttccaga tgcgaaaacg acacgttaca acgctgggtg gctcgggatt gcagggtgtt
    20761 ccggagacct ggcggcagta taggccgttc acaaaatcat tacaattaac ctacatatag
    20821 tttgtcgggt tttatcctga acagtgatcc aggtcacgat aacaacattt atttaatttt
    20881 taatcatcta atttgacaat cattcaacaa agttgttaca aacattacca ggaaaagcat
    20941 ataatgcgta aaagttatga agtcggtatt tcacctaaga ttaacttatg taacagtgtg
    21001 gaagtattga ccaattcatt cgggacagtt attagtggta gacaagttta ataattcgga
    21061 ttgctaagta cttgattcgc catttattcg tcatcaatgg atcctttacc tgcaagcgcc
    21121 cagagctctg tacccaggtt ttcccctctt tcacagagcg gcgagccaaa taacaaacgg
    21181 gtaaagccag gttgatgtgc gaaggcaaat ttaagttccg gcagtcttac gcaataaggc
    21241 gctaaggaga ccttaaatgg ctgatacaaa agcaaaactc accctcaacg gggatacagc
    21301 tgttgaactg gatgtgctga aaggcacgct gggtcaagat gttattgata tccgtactct
    21361 cggttcaaaa ggtgtattca cctttgaccc aggcttcact tcaaccgcat cctgcgaatc
    21421 taaaattact tttattgatg gtgatgaagg tattttgctg caccgcggtt tcccgatcga
    21481 tcagctggcg accgattcta actacctgga agtttgttac atcctgctga atggtgaaaa
    21541 accgactcag gaacagtatg acgaatttaa aactacggtg acccgtcata ccatgatcca
    21601 cgagcagatt acccgtctgt tccatgcttt ccgtcgcgac tcgcatccaa tggcagtcat
    21661 gtgtggtatt accggcgcgc tggcggcgtt ctatcacgac tcgctggatg ttaacaatcc
    21721 tcgtcaccgt gaaattgccg cgttccgcct gctgtcgaaa atgccgacca tggccgcgat
    21781 gtgttacaag tattccattg gtcagccatt tgtttacccg cgcaacgatc tctcctacgc
    21841 cggtaacttc ctgaatatga tgttctctac gccgtgcgaa ccgtatgaag ttaatccgat
    21901 tctggaacgt gctatggacc gtattctgat cctgcacgct gaccatgaac agaacgcctc
    21961 cacctccacc gtgcgtaccg ctggctcttc gggggcgaac ccgtttgcct gtatcgcagc
    22021 aggtattgct tcactgtggg gacctgcgca cggtggtgct aacgaagcgg cgctgaaaat
    22081 gctggaagaa atcagctccg ttaaacacat tccggaattt gttcgtcgtg cgaaagataa
    22141 aaatgattct ttccgcctga tgggcttcgg tcaccgcgtg tacaaaaatt acgacccgcg
    22201 cgccaccgta atgcgtgaaa cctgccatga agtgctgaaa gagctgggta ccaaagatga
    22261 cctgctggaa gtggctatgg agctggaaaa catcgcgctg aacgacccgt actttatcga
    22321 gaagaaactc tatccgaacg tcgatttcta ctctggtatc atcctgaaag cgatgggtat
    22381 tccgtcttct atgttcaccg tgatcttcgc tatggcacgc accgttggct ggatcgccca
    22441 ctggagcgaa atgcacagtg acggtatgaa gattgcccgt ccgcgtcaac tgtatacagg
    22501 atatgaaaaa cgcgacttta aaagcgacat caagcgttaa tggttgattg ctaagccgtt
    22561 tactttccgg acccgccttt aatagagcgg gttctttttt atatggctga acataataaa
    22621 ttgtaaaaat taaaccagat gcaaataccc attaataagg tgaaaaaatg tgacaagtca
    22681 catactgagc agaatgataa gtttattttt ttactttcct gccggtagca ttgttaaccc
    22741 aatgaaaatt atgcatatcc atcccacccc atctttcatc tcattaaaca ataaatttat
    22801 gcactcaatt attccattct ttatataaca agctttttaa tattgttaaa taaaaccaaa
    22861 tacattcaac tgtcagttaa tatattcttg cctcgcaaca ctctatctca ttatttaaag
    22921 gactcagcta tgtttaaagg acaaaaaaca ttggccgcac tggccatatc tctgctattc
    22981 acagcacctg tttatgcggc tgatgaagga tccggtgaaa ttcactttaa aggtgaagtt
    23041 attgaagcac cgtgtgaaat acatcaggat gatattgata aagaggttga actcggtcag
    23101 gtgaccacca gccacattaa tcagtcgcat cacagcgatg ccgttgctgt cgacctgcgc
    23161 ttagtcaact gcgatctgga aaactccaac aacggttccg gcggcaagat ttctaaagtt
    23221 gcagtcacct ttgacagctc agcgaaaacc accggcgcag atccgattct caacaacacc
    23281 agcacgggtg aagcgaccgg tgtcggcgtg cgtttaatga ataaagatca aagcaatatc
    23341 gttttaggca ccgctactcc agatatcgac ctggcaccaa cctccagcga acaaacgtta
    23401 aatttctttg cgtggatgga acaaattgat caggctacgc ccgtaacacc aggcgcagtt
    23461 acggcgaatg cgacctacgt actggattat aaataagtat cactttggcg gcgtcatcgt
    23521 cgcctttctt caaatattta tcaggtatgg acaccgtgaa tatttatcga ctctctttta
    23581 tatcctgtct ggtcatggcg atgccttgcg cattggcggt cgaattcaac ctgaatgtcc
    23641 tcgataaatc gatgcgagat cgcattgata tttcattatt aaagggaaaa ggggtcattg
    23701 ttcccggtga atattttgtt agcgttgcag taaataacaa ccaaatcagt aacggacaaa
    23761 aaattgactg gaaaaagaac ggtgaccaaa ccattccgtg tattaatgat ttactggtag
    23821 ataaatttgg tttaaaacct gaagttcgtc agtcgttacc acggttaaac cagtgcgtcg
    23881 attttagctc ccggcctgaa attctcttca tttttgatca ggctagccag caactgaata
    23941 tcactattcc acaagcctgg ctggcgtggc actcagataa ctggactccc ccctcaacat
    24001 ggaaagaagg tgtcgccggc gtcctgatgg actacaacct gtttgcaagc agctaccgcc
    24061 cacaggatgg tagcaacagc actaacctga acgcctatgg aacagccgga attaacgcgg
    24121 gagcatggcg tttacgcagt gattaccaat tgaatcagac cgatagcgat gataaccatg
    24181 aacagtcagg cgaaatatca cgcacctatc tttttcgtcc attaccgcaa ttaggttcaa
    24241 aattaaccct tggtgaaacg gatttcagtt ccaatatttt cgacggcttt tcttataccg
    24301 gcgcggcact ggcaagtgac gatcggatgt taccgtggga gctacgcggc tacgccccac
    24361 aaatcagcgg cattgcacaa accaacgcca ccgtcaccgt gagtcactca gggcgagtta
    24421 tttaccagaa gaaagtccca ccaggtccgt ttatcattga tgaccttaat cagtccgtcc
    24481 agggtacgtt agatgtcaaa gtgacggaag aagatggtcg ggcgaacaat ttccaggttt
    24541 cggcagcatc gacgcctttc cttacgcgcc aggggcaggt tcgctataaa cttgctgccg
    24601 gtcagccgcg aacatctatg tctcatcaaa ctgaaaatga aacctttttt agcaatgaag
    24661 tttcctgggg gatgctgtca aatacctcac tgtacggcgg cctgctgatt tcgggtgacg
    24721 actaccattc tgccgcaatg ggtatcgggc aaaatatgct gtggcttggt gcattgtcgt
    24781 ttgatgtcac gtgggccagt agtcagtttg atactcagca ggacgagcgg ggcttaagtt
    24841 accgctttaa ttacagcaaa caagtggatg ccaccgacag cacgatttcg ctcgccgctt
    24901 atcgtttctc cgatcgtcat tttcacagct acgccaacta tctggatcac aaatacaaca
    24961 acagcgatgc gcaggacgaa aaacagacga tcagcttatc cgtgggccaa ccgattaccc
    25021 cactgaacct caatctgtac gccaacctgc tgcatcaaac ctggtggaat gcagacgcct
    25081 ccacgaccgc caacatcacg gcgggtttta atgttgatat tggtaactgg agagatatct
    25141 cgatctcgac gtcattcaat acaacccatt acgaagataa agatcgcgac aatcagattt
    25201 acctgtcgat ttcgctcccc ttcggtaacg gggggcgggt cggttatgac atgcaaaaca
    25261 gtagtcacag caccacgcac cgcatgtcgt ggaatgatac gttagatgaa cgtaatagct
    25321 ggggcatgtc taccggactg caatccgacc gtccggataa tggagcccag gtgagcggta
    25381 actatcagca cctgagttca gcgggtgagt gggatatttc tggtacctat gccgccaatg
    25441 attacagttc cgtcagcagc agctggagcg gttctttcac ggcaactcaa tatggtgcgg
    25501 cgttccatcg tcgcagctcc accaatgaac cacgtctgat ggtcagtacc gatggcgtgg
    25561 cagatattcc ggttcagggc aatctcgact acaccaacca ttttggcatt gcggtggtgc
    25621 cgttgatttc cagttatcag ccttccaccg tggcggtgaa catgaatgac ttacccgacg
    25681 gcgtaacagt ggcagaaaac gttatcaaag aaacgtggat tgaaggcgcg ataggttaca
    25741 aatcgctggc ttcccgttcc ggtaaagacg ttaacgtcat cattcgcaac gccagcggtc
    25801 agttccctcc cctcggtgcg gatatccgcc aggataacag cggcattagc gtggggatgg
    25861 ttggcgagga aggacatgcc tggttaagcg gagtcgctga aaatcaacaa tttaccgtga
    25921 tctggggtga tagccagcgt tgctcgctcc atcttcctga acatatggaa gacaccgcaa
    25981 atcgcctgat tttaccttgt cattaattaa ggaaatgaaa atgacattta tgaaaggact
    26041 accccttttg ttgttggttg ccagtctgtg cagccacgct gcactacaac ccgatcgcac
    26101 tcgtattgtg tttaacgcta atgataaagc taccagtctg cgcgtggata atcgtagcga
    26161 taaactcccc tacctcgctt attcctggct cgaaaatgaa aaaggggaga aaagcgatga
    26221 tcttctggtt gccctgccac cgatacaacg tctggaaccg aaagcaacaa cgcaggtacg
    26281 gatagtaaag caagcctcga ccacaaaatt accgggcgat cgcgaaacgc tatttttcta
    26341 caatatgcgt gaaattcctc cctccccaga aaaaaacagc ggccatgccg ttctgcaagt
    26401 cgccattcag agtcgtatca aggtattctg gcgaccggct gcattacgca agaaagcagg
    26461 agaaaaggtc gaactgcagt tacaggtcag ccagcagggc aaccaactga cactgaaaaa
    26521 tcctaccgcg tattatctga ccattgccta tctggggaga aatgaaaaag gcgttcttcc
    26581 cggcttcaaa accgtgatgg ttgcgccttt tagtacagta attacaaaca ccggaagtta
    26641 tagcggcagt cagttttatc tcggttatat ggatgattac ggcgcgttac gcatgacgac
    26701 gctcaactgc agcggacaat gccgtttaca agcagtggag gcgaagaaat gagtgctggc
    26761 aagggagtgt tgctcgtcat ctgtctggta tttctgccat tgaagtctgc gctagcgctg
    26821 aactgctatt ttggtacttc cggcggaacg gtagaaaaat cggaagcgat tcaacccttt
    26881 gctgtgccag gtaatgccaa acccggcgat aagatctggg aatctgacga tattaaaatt
    26941 cccgtctatt gtgacaacaa taccaacggc aactttgaaa gcgaacatgt ctacgcatgg
    27001 gtaaacccct acccgggggt acaggatcgt tattatcaac tcggcgtgac atacaatggt
    27061 gtcgattacg atgccagtct ggggaaaagc cgcatcgaca ccaaccagtg tatcgacagt
    27121 aagaacatta acatttatac ccctgagcag attatcgcga tgggctggca gaacaaaata
    27181 tgctcgggtg accccgctaa tattcacatg tcgcgaacct ttcttgctcg tatgcggtta
    27241 tacgttaaga ttcgcgaaat accgccgcat gattatcaaa gttcgcttag cgactacatc
    27301 gtcgtgcaat ttgatggtgc cggtagcgtt aacgaagacc ctaccgccca aaacctgaaa
    27361 taccatatta ctggtctgga aaacattcgt gtactggatt gcagcgtcaa tttttctatt
    27421 tcaccagaaa cacaggtgat tgattttggt aaatttaacc tgctggatat tcgccgccac
    27481 acaatgtcga aaacattcag tattaagacg acaaaatcac aaaatgacca gtgcaccgac
    27541 ggatttaagg tcagctcttc gttttatacc gaagaaacat tgattgaaga ggataaagca
    27601 ctgctgattg gtaatggact gaagctacgt ttgctggatg aaaattcttc accctatact
    27661 ttcaacaaat acagcgaata tgctgatttc accagcgata tgctgatcta cgaaaaaacc
    27721 tatacggctg aactttcgtc cattcccggc acccccatcg aagcgggtcc cttcgataca
    27781 gtggtgcttt ttaagattaa ctataactaa tttatcatga tgccctttgc agttagtaaa
    27841 gggcctctct ttcagaaaat tgctgatttt ccccttttcc ttgattgctg ttattttttc
    27901 ctggcccggc tttctttatg ctgtatcgat acaccaaaga aaaaataatg agggaacgaa
    27961 ggatgccagt tttgcagtgg ggaatgttat gtgtgctctc acttctcctt tcaattggtt
    28021 tcctcgcggt tcatctcccg gcggcgctat tacttgggcc gatgatcgcc gggatcatct
    28081 ttagtatgcg cggaattact ctgcaacttc cccgctccgc ttttctcgcc gcccaggcca
    28141 ttcttggctg catgattgcg caaaacctga ccggttcgat cctgaccacg ctagcggtga
    28201 actggccgat tgtgttagct attttactgg tgacgttact ttccagcgcc atcgtgggtt
    28261 ggttattggt gcgctatagc tcactgcctg gaaataccgg tgcctggggt tcttctcctg
    28321 gcggcgcggc ggcaatggtc gctatggcgc aagattacgg cgcagatatt cgcctggtgg
    28381 cgtttatgca atatctgcga gtgctgttcg tcgcaggggc cgctgttctg gtcacccgca
    28441 tgatgctggg cgataacgct gaagcggtta accagcagat cgtctggttt ccgccagtga
    28501 gcattaatct cctgcttacg attttgctcg cggtcgttgc cggtacagtg ggatgcttgc
    28561 tacgtcttcc ttccggcacg atgctcatcc cgatgctggc gggcgcagtg ctccagtctg
    28621 gtcagctcat caccatcgaa ctaccggaat ggctgcttgc gatggcgtat atggcaattg
    28681 gttggcggat tggtcttggt ttcgataaac aaatcttact gcgggcattg cgcccgctac
    28741 cgcaaatcct gctgtcgatt tttgctctgc tggctatttg tgcgggtatg gcgtgggggc
    28801 tgacccggtt tatgcatatt gattttatga ccgcctacct cgccaccaac cccggcgggc
    28861 ttgataccgt ggcggtcatc gccgcaggga gcaatgccga tatggcgctc atcatggcga
    28921 tgcaaaccct gcgcctgttc agtattttgc tgacgggacc tgccattgcg cggtttattt
    28981 caacctatgc gccgaagcgt tctgcctaat gctggcagcc agggcaccag taaaacgggc
    29041 gagatgacag cgtggttttc tcaatgatgc cgccacagcg ttcgcacagt tcgccatcac
    29101 gatgaaaaac cttaaagcga aacagcgccc catgatgctt attctcatcc acctgccccc
    29161 gcgtagcgta ggaaaatcga ggaatatcca gtaacgcgtg tgcgagtgca tccagttgcg
    29221 ccgcattgag atctttcgct ttatgatttc cagtcaaccc aacctgccag aggatctcca
    29281 cccgcaaata attgccaagc ccagccagaa acgcctgatc gagcagtaat ccagcaaact
    29341 gacggttacg aaagcgcggc gacaataacc gttcctttac cacctccggc gtcagattcg
    29401 gatccagcac atcggggccg actcgttgta aaaacggatg cgtgatcagt tgttccggcg
    29461 tcaacatctc aatatccgag gcgctataaa gcagaatagt tttgtcaacc gtttgcagtt
    29521 ttacccgcaa tactcgcata gtctgcggct cttcgccggt atcaaccacg cgccagacgc
    29581 cgtaaagctg attatggctg tagagcgtta agtcgttgga aaaatgagtt aacaacgcct
    29641 taccacgcgt ttccacatgg gtaacgtgtt gaccgataag ttgtgattga taaggtttta
    29701 actgcgggaa ggcaaaccag acatcagtta gtggtttgcc tttgatcgcc gcctccaggt
    29761 tatccgctgc acggcggatc tccgggcctt caggcatggt gatatccttt tttcattctt
    29821 tattaaacgg ggttatgctg cgacaacaat ccccttttcg gcaaatgtag agcgtagtcg
    29881 gcgggcgaat gccagtgcgt gctcgccgtc gccatgcagg cagaccgttt gcgccgtgac
    29941 cgttgcccat tcgccggtga tgcttttgac tctgccgtgt tgcaccatct ccagcgtttg
    30001 cgccagcgcc tgttcttcgt tttcaatcaa cgcgcctggc tggcttcgcg gcaccagcga
    30061 accgtctgcc tgataaccgc gatcggcaaa cacttcctcg cgcgttgtca ggccatattg
    30121 cttgcctgca cgaatcagct cgcttcctgc cagcccgacg agaatcaatg ccggatcgca
    30181 agcgtatacc gctctggcga tggcgtctgc cagttgtgcc tctttcgctg cctggttgta
    30241 taacatgccg tgaggtttga catgacgcat aacaccgcct tgcgcacggg caatcgttgc
    30301 cagcgcgcca atttgataca gcgtctgggc gtaaacggtt tctggcggca gctgcatgac
    30361 gctgcgccca aaattttccc tgtcgggaaa actcgggtga gcgccaatcg caacaccatt
    30421 ttttattgct tcacgcacgc aagcctgcat ggtttgcgca tcgcctgcat gaaatccaca
    30481 ggcaatattg gcagaggaaa ccagcgttaa tagctctgcg tcgctggcat agccttcgcc
    30541 cagatcggcg ttcaggtcaa ttttcattgt gcagccgcca cgctaattgt tcgaaataac
    30601 gttgctgatc ttgccgtgct ttcagtgctt cttccagtga acactggaca aaatgaatcg
    30661 gctgaccgag cggaatttgc gccagatggt acatatcagc ctcaatgata caggcaatac
    30721 gcggataacc accggtggtc tgtgcgtcgt tcatcaacac aatcggctgc ccgttatgcg
    30781 gcacctgcac cacgcccggt aacaaaccat gagataacag ttcgcgatcg gtggtacgtt
    30841 ttaaaatttg cccctgtaag cgatagccca ttcggttact ttgcgagcta agctgccagg
    30901 gcgaacgcca gaatgcatcc tgcgaggcgc gatcgaactc atgatattcc ggccccggca
    30961 aggcgcgaat gcggttgccc cacagcagct gtttaacgcc ctgcgcttcc ataaaatcac
    31021 gcttcgattt gccaatcggg agtcggtcac catccttcag taaacggcct tccagcccgc
    31081 caatccccac tttgagatcg gtgctgcatg accccattac cggcggaaca tcaataccac
    31141 ccgcgaccgc cagataactg cgcatcccgt gctgcgggcg tttaagcgtt aaacgctgac
    31201 ctgctttcat cggcaagcgc cagccggtcc agacggcgtt atcatccagc cgcgcttcgc
    31261 aaccggcccc cgtcagagca aaccacccat cagtttcgaa ctcaaccgtt aactgaccga
    31321 gcgtgatctc cagcgcgggg gcattggcgt cattacccac cagtaggtta gcaatgcgca
    31381 gcgcaggcat atccagtgcg ccgcagtggc tgatacccga ctggcgaaaa ccgtgacgac
    31441 cgccatcctg cacagtggta tacatacccg cacgaataat cttcagcata ctccctcctt
    31501 ctgcggtaca aagcgcacgc tgtctcccgg acgtaataag atgggttcgt cacgcgccgg
    31561 atcaaacagg ctgagtgagg tatgaccaat caactgccag ccacccggcg ttgccaacgg
    31621 ataaatacca gtctgcggcc cgccgatccc gacagaacct gccggaacga gcaagcgcgg
    31681 ttcagcgcgc cgtggcgtgt gtagttgttc cggcaaactc ccgagatacg ggaagcccgg
    31741 ttgaaaacct aaaaaccaga ccacgtattc cacggaggag tgcaattcaa caacctgttt
    31801 ttcgctcaat ccacaatgcg ccgcgaccac cgccaaatcc ggtccggctg cgccaccgta
    31861 aaccaccgga atttcaataa agcgagactc cggctccagc gcctcactct cctcccacca
    31921 gcgttgcaaa cgctctatgg catccagcgc cagcgactca ggattacgca aaatcactgt
    31981 gatattgttc atgccaggaa tggcttcaac cacattcggc atatccacca gacgctgtgc
    32041 cagtcgccag atccgtttct ggctagccag cgtcaccggc ggttccagct ccagcactac
    32101 cgccgtttca cctatcagat aacaacgcgc tcgttgcact ttcgatccct ctgattattc
    32161 gttatgcagg attaggaata tcaataaagg tcacatcaag atcggtattt tcattcagcc
    32221 actcgctcaa tgcgcgaata ccaccgcgtt cagtggcatg gtgacccgca gcataaaaat
    32281 gcaatccctg ctcgcgggct gaatgaatgg tctgttctga aacttcgcca gtaataaaag
    32341 catccacgcc aaaacgcgcg gcgctatcga taaaactctg cccgccgccc gtgcaccagg
    32401 cgacgcgctg aaccacctca ggtccggtat cgccacacca taacggctta cgtcccagac
    32461 gcgcttcaat ccaggaagcc agctccagtc ccggcacagg catggtcagt tcgccccacg
    32521 gcaccaacgg ctcaatttcg cccatgaccg tgatccccag taacgccgcc agttgtgcgt
    32581 tattacccag ctccggatgc gcgtccaggg gcagatgcca gccatacagg ttgatatcat
    32641 tcgccagcaa cgtttttaaa cggttacgct tcattccgcg aatgaccgga gattcacctt
    32701 tccagaagta gccatgatgc acaatgaccg catccgcgcc cagacgcacc gcttcatcga
    32761 gcaaagcctg gctggcggtg acaccggtaa caattttttg caccgtctct ttgccttcca
    32821 cctgcaaacc attcggcgcg taatcactaa tcgccgcgct gttcagtttt tcgttaatca
    32881 gttgttccag ttcggtgttt ttcatcttcg cgccttccgt attctgatag ctacaccgtt
    32941 aacatagcgt tacctcgcct gttcgtcgat agccaatctt ttgacatatt aagaaacaaa
    33001 cccgacgcca aaagacgcaa attgtttata tcatcacgct tcagctaatg tatatttatg
    33061 caatcagaca gcatattgca taaataatca gatctgtatc aaagttttaa ctttaaggaa
    33121 acacaagcac gccgcgcaac tattacgtga cgttgcagtc aattgtggaa ttattatgaa
    33181 taaacacgca tcacaaccgc gcgctattta ctatgtcgtt gcgctgcaaa tctgggaata
    33241 ttttagcttt tacggcatgc gtgccctgct gatcctctat ctcaccaatc aactaaaata
    33301 tagcgataat cacgcctacg agttatttag cgcctactgt tcgctggtat atgtcacgcc
    33361 aatcctcggt ggctttttgg cggataaagt tctcggcaat cgcatggcgg tgatgctggg
    33421 tgcgttgttg atggcgatcg gtcatgtggt gctgggtgcc agtgagatcc atccgtcatt
    33481 cctctatctg tccctggcga ttatcgtttg tggctatggt ctgtttaaat ccaacgtcag
    33541 ttgcctgctc ggcgagctgt atgagccaac cgatccgcgt cgtgatggcg gtttctcgct
    33601 gatgtatgcg gcgggtaacg tggggtctat tatcgcacct atcgcctgtg gctacgccca
    33661 ggaagagtac agttgggcga tgggctttgg cctggcggcg gttggcatga tcgcgggtct
    33721 ggtcattttc ttatgtggca atcgtcattt ctctcatacc cgcggcgtta acaaaaaagt
    33781 actgcgtgcg acaaactttc tcctgcccaa ctggggatgg ctgctggttc tgctggtggc
    33841 aacacctgcg ctgatcaccg tactgttctg gaaagagtgg tcggtatacg ccttgattgt
    33901 cgcgactatt attggcctgg gtgtattggc aaaaatttat cgcaaagcag aaaaccagaa
    33961 acagcgtaag gagctggggc tgattgttac cctcaccttc ttcagcatgt tgttctgggc
    34021 attcgcacaa caggggggta gctcgattag cctttatatc gaccgcttcg ttaaccgcga
    34081 tatatttggt tataccgttc cgactgctat gttccagtcg attaatgcct tcgcagttat
    34141 gctgtgcggt gtgttcctgg cgtgggtggt taaagaaagc gtcgcaggta atcgtaccgt
    34201 gcgcatctgg gggaaatttg ctcttggtct cggcctgatg agcgccggat tctgcattct
    34261 gaccttaagc gcccgctggt ctgcaatgta tggtcactct tctctgccac tgatggtgtt
    34321 aggcctggcg gtgatggggt ttgcggaact gtttatcgac ccggttgcta tgtcgcagat
    34381 tacgcgcatt gaaatccccg gcgtgaccgg cgtattaacc ggcatttata tgctgctttc
    34441 cggcgcgatt gcgaactatc tggccggcgt gattgccgat cagacatcgc aggcttcgtt
    34501 tgatgcttcc ggagcgatca actactccat caatgcatat attgaagtat ttgatcaaat
    34561 tacctggggc gcactggcgt gtgtaggact ggtgctgatg atttggctgt atcaggcgct
    34621 taagttcaga aaccgcgcgc tggcgctgga atcttaacag gccagcaccg atgcctgata
    34681 agacgctcgc tgcgttttat caggctcctt tcctcgcttc ttcgtacgct gccagcgttc
    34741 gcagccttgc ctctttgtga tcaactatcg gttgcggata atccagcgtc acacctgctt
    34801 tctgcgccca cttccacggc tcatgcacca ctttccctgg cacatcgcgc agttccggta
    34861 gccactggcg gataaactcg ccctcacggt caaatttctc gccctgggtt gtcgggttaa
    34921 aaatacgaaa atacggcgct gcatcggttc cggttgaagc ggcccactgc cagccaccgt
    34981 tattggctgc caaatcacca tcaatcagct gcgacatgaa atatcgctca ccttcgcgcc
    35041 agtcgatcaa taaatctttc accaaaaaac tggctgtaat catccgtagc cggttatgca
    35101 tccagccggt gctgttaagc tgacgcatgg cagcatcaac aatcgggtat cccgttttgc
    35161 cttcctgcca ggcctttaaa tgcgcggaat tgctctgcca ctgcacgcgg tcggtccaga
    35221 caataaacgg acgatgttta cacagcgagg ggtaatacgt tatcagatga cggtaaaact
    35281 cgcgccagat cagctcatta agccagatac taccggcccc accgtccagc gcctgcggct
    35341 gttccgccaa caggcgatgc aaacactggc gaggcgataa cccgcccgtt gccaggctgg
    35401 cggacaaacg gctggtgcct tctaccgcag ggaaatctcg ctgttgctca tactctccgg
    35461 caccgttctg gcaaaactgg cgtaattgcg caatcgccgc tttctcttcc accgggaaat
    35521 gcgcagtatc gaaaggctga cgaggataat tcagcgtaat ggatggcgcg ggatctatcg
    35581 atccgctact acgtactttt ggcgcagcga cgcactccgg catcccttct cgcagccgtt
    35641 taagccaggc gtttttaaat ggcgtaaaga ctttgtacat ctcatgattg ccagtcatca
    35701 ccgcccccgg cggcaggatc acgccgtcgt caaatccttc acacaccacg ttacgcagcg
    35761 ccctctcagc ctgcacatcc cgcgcccgct cattcacctc atactgatag ttataaaaca
    35821 gatgggtaac acgattttcc gcgcacacct gtttaactat ttcgacgctg gcggcaaagt
    35881 catccacttc atgaaacagc aaaggaatgc ctttttccgc cagcgcgatt tgtagcccgt
    35941 tcagttgtgc attgataaat tcagcctgac gcggcgacat attatgcgcc gcccactggc
    36001 gtggtgtagc gatatacaac gccagcacgc ttgcagacga attgcggcag gcggcagcca
    36061 gcgcgagatt atcatgctga cgtaaatcct ggcgaaacca gaccagatgg gtagtcataa
    36121 aactcctgaa tggcgcaagt taatcacacg cggccaaagt tcgaaataac gctgccctga
    36181 aagccaggcg tcaggataaa gcgccatata gtgtttgata cgcatcagcg gcgcaagaag
    36241 cggttgctcg ccaccgcgat aactgtcgat aagcgccgcc agctgctggc gttctgtgga
    36301 atcaatatga gggcgaaaat aaccctgaac gtgcatcaaa acattggtgt ggtcacgtcg
    36361 gttggcgggg tgagaaagca aaacaactac gcgttggcgg tactggttgt aatagtcatc
    36421 cagattttgc cactggtgaa tatcagccac gaacgggcca agtttgcggt actcgggctg
    36481 ggaatgggcc agaaagacga gtttatagcg gctgtgataa tcgttgagtg ccccgcgcgt
    36541 taagccatgt tctttcagtc gatgtaactc ataaagcgcg aagatacgga ttagagtgct
    36601 ttcatcaaat cgttgctgat tcattactgc tcctgagata ttaacaggag taagtgtaga
    36661 cgttggcaaa tgatgtttgt gaagttaatc ctgttgtagc agggcaaaat tacgaaagca
    36721 cgctccccgc aaggactgac gccagatatt ttctgtccat ggctgccctt tccattcaaa
    36781 cgtgaaatca ataagttcat cagggacatc gcaaacaata tgaagacgga tttcttctct
    36841 gccgtgactc ttttcactca ttgcgtaact gccatgctct ggattattta attctttcag
    36901 cggaaatttt tcctcgaagg ctttatttag ccgcccctgg tttcctttta cagcgaataa
    36961 ataatcaccg ccctgttttt gtatcttctc tgcaatatct ttctggcaac ccatcgcatc
    37021 agttgtgatg atttttcctt taatattcag catgttaaga agttccggga tagctgtgat
    37081 ctcattggat ttctcatccg tcttgatctg tccgatgacc agactgtgca ttgttgagaa
    37141 cgcactaatg acatgaatcg ctcccttgcg gcgactcttg tcataagagt gccggagcgt
    37201 ttttccatca attgcaatga cgtctttatc atctgaagaa tggcagtcac gcatccagtt
    37261 aataaagcac tcgtgaaatt ttgcaggact gatacaggat acaactctgg caatggtatc
    37321 gtgaacagga ataccatttt caaaat
//
LOCUS       DAZGHF010000031        37244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGHF010000031 DAZGHF010000000
VERSION     DAZGHF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.031"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..385
                     /locus_tag="ACHK3A_001701"
     CDS             <1..385
                     /locus_tag="ACHK3A_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO8739791.1"
                     /translation="LLTAEKARGMKGDEINAWRNAMEAANYMFEHNGRKWDYGKSTQT
                     RLEPSVAAAKAGKLPEAFFWTDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQR
                     TMKKELEKLTSADEILAYRVGWAQE"
     gene            complement(544..873)
                     /locus_tag="ACHK3A_001702"
     CDS             complement(544..873)
                     /locus_tag="ACHK3A_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO8739792.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KYGLPEI"
     gene            complement(1013..1100)
                     /locus_tag="ACHK3A_001703"
     tRNA            complement(1013..1100)
                     /locus_tag="ACHK3A_001703"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1064..1066),aa:Ser,seq:tga)
     gene            1527..2645
                     /gene="hyaA"
                     /locus_tag="ACHK3A_001704"
     CDS             1527..2645
                     /gene="hyaA"
                     /locus_tag="ACHK3A_001704"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO8739793.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     KMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2642..4435
                     /gene="hyaB"
                     /locus_tag="ACHK3A_001705"
     CDS             2642..4435
                     /gene="hyaB"
                     /locus_tag="ACHK3A_001705"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO8739794.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLSDDGSELISVQVR"
     gene            4454..5161
                     /gene="hyaC"
                     /locus_tag="ACHK3A_001706"
     CDS             4454..5161
                     /gene="hyaC"
                     /locus_tag="ACHK3A_001706"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO8739795.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDVHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5158..5745
                     /gene="hyaD"
                     /locus_tag="ACHK3A_001707"
     CDS             5158..5745
                     /gene="hyaD"
                     /locus_tag="ACHK3A_001707"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO8739796.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPAKESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            5742..6140
                     /gene="hyaE"
                     /locus_tag="ACHK3A_001708"
     CDS             5742..6140
                     /gene="hyaE"
                     /locus_tag="ACHK3A_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO8739797.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGS
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6137..6994
                     /gene="hyaF"
                     /locus_tag="ACHK3A_001709"
     CDS             6137..6994
                     /gene="hyaF"
                     /locus_tag="ACHK3A_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO8739798.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQQQISERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            7128..8672
                     /gene="appC"
                     /locus_tag="ACHK3A_001710"
     CDS             7128..8672
                     /gene="appC"
                     /locus_tag="ACHK3A_001710"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO8739799.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8684..9820
                     /gene="appB"
                     /locus_tag="ACHK3A_001711"
     CDS             8684..9820
                     /gene="appB"
                     /locus_tag="ACHK3A_001711"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO8739800.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9833..9925
                     /gene="cbdX"
                     /locus_tag="ACHK3A_001712"
     CDS             9833..9925
                     /gene="cbdX"
                     /locus_tag="ACHK3A_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO8739801.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10005..11303
                     /gene="appA"
                     /locus_tag="ACHK3A_001713"
     CDS             10005..11303
                     /gene="appA"
                     /locus_tag="ACHK3A_001713"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO8739802.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQCLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFSQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11423..13603)
                     /gene="etk"
                     /locus_tag="ACHK3A_001714"
     CDS             complement(11423..13603)
                     /gene="etk"
                     /locus_tag="ACHK3A_001714"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO8739803.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIVSVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEGFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13623..14069)
                     /gene="etp"
                     /locus_tag="ACHK3A_001715"
                     /gene_synonym="yccY"
     CDS             complement(13623..14069)
                     /gene="etp"
                     /locus_tag="ACHK3A_001715"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO8739804.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(14057..15196)
                     /gene="gfcE"
                     /locus_tag="ACHK3A_001716"
     CDS             complement(14057..15196)
                     /gene="gfcE"
                     /locus_tag="ACHK3A_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO8739805.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15242..17338)
                     /gene="gfcD"
                     /locus_tag="ACHK3A_001717"
     CDS             complement(15242..17338)
                     /gene="gfcD"
                     /locus_tag="ACHK3A_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO8739806.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17338..18084)
                     /gene="gfcC"
                     /locus_tag="ACHK3A_001718"
     CDS             complement(17338..18084)
                     /gene="gfcC"
                     /locus_tag="ACHK3A_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO8739807.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVVATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWLAGRSVTDYLQDHTRLAGADKNNVMVITPEGEAVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(18081..18725)
                     /gene="gfcB"
                     /locus_tag="ACHK3A_001719"
     CDS             complement(18081..18725)
                     /gene="gfcB"
                     /locus_tag="ACHK3A_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO8739808.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18832..19137)
                     /locus_tag="ACHK3A_001720"
     CDS             complement(18832..19137)
                     /locus_tag="ACHK3A_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739809.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19578..19790)
                     /gene="cspH"
                     /locus_tag="ACHK3A_001721"
     CDS             complement(19578..19790)
                     /gene="cspH"
                     /locus_tag="ACHK3A_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO8739810.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            20076..20288
                     /gene="cspG"
                     /locus_tag="ACHK3A_001722"
     CDS             20076..20288
                     /gene="cspG"
                     /locus_tag="ACHK3A_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO8739811.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20299..20487
                     /gene="ymcF"
                     /locus_tag="ACHK3A_001723"
     CDS             20299..20487
                     /gene="ymcF"
                     /locus_tag="ACHK3A_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO8739812.1"
                     /translation="MTPHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20462..20692
                     /gene="ymcE"
                     /locus_tag="ACHK3A_001724"
     CDS             20462..20692
                     /gene="ymcE"
                     /locus_tag="ACHK3A_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO8739813.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            20682..20855
                     /gene="gnsA"
                     /locus_tag="ACHK3A_001725"
     CDS             20682..20855
                     /gene="gnsA"
                     /locus_tag="ACHK3A_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO8739814.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20903..21976)
                     /gene="yccM"
                     /locus_tag="ACHK3A_001726"
     CDS             complement(20903..21976)
                     /gene="yccM"
                     /locus_tag="ACHK3A_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO8739815.1"
                     /translation="MTEKRRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDTPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLNFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLILGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(22048..24792)
                     /gene="torS"
                     /locus_tag="ACHK3A_001727"
     CDS             complement(22048..24792)
                     /gene="torS"
                     /locus_tag="ACHK3A_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO8739816.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     DFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKANQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPQRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLLLRIATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAVALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDDIDIARANQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            24875..25903
                     /gene="torT"
                     /locus_tag="ACHK3A_001728"
     CDS             24875..25903
                     /gene="torT"
                     /locus_tag="ACHK3A_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO8739817.1"
                     /translation="MRVLLLLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMAEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQEQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(25876..26568)
                     /gene="torR"
                     /locus_tag="ACHK3A_001729"
     CDS             complement(25876..26568)
                     /gene="torR"
                     /locus_tag="ACHK3A_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO8739818.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26698..27870
                     /gene="torC"
                     /locus_tag="ACHK3A_001730"
     CDS             26698..27870
                     /gene="torC"
                     /locus_tag="ACHK3A_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO8739819.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            27870..30416
                     /gene="torA"
                     /locus_tag="ACHK3A_001731"
     CDS             27870..30416
                     /gene="torA"
                     /locus_tag="ACHK3A_001731"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO8739820.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKEGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGINAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            30413..31012
                     /gene="torD"
                     /locus_tag="ACHK3A_001732"
     CDS             30413..31012
                     /gene="torD"
                     /locus_tag="ACHK3A_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO8739821.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATQTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDNLRQKTLTA
                     LRQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(31267..31572)
                     /gene="cbpM"
                     /locus_tag="ACHK3A_001733"
     CDS             complement(31267..31572)
                     /gene="cbpM"
                     /locus_tag="ACHK3A_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO8739822.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(31572..32492)
                     /gene="cbpA"
                     /locus_tag="ACHK3A_001734"
     CDS             complement(31572..32492)
                     /gene="cbpA"
                     /locus_tag="ACHK3A_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO8739823.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            32645..32746
                     /locus_tag="ACHK3A_001735"
                     /pseudo
     CDS             32645..32746
                     /locus_tag="ACHK3A_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33027..34268
                     /gene="agp"
                     /locus_tag="ACHK3A_001736"
     CDS             33027..34268
                     /gene="agp"
                     /locus_tag="ACHK3A_001736"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO8739824.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            complement(34306..34533)
                     /gene="yccJ"
                     /locus_tag="ACHK3A_001737"
     CDS             complement(34306..34533)
                     /gene="yccJ"
                     /locus_tag="ACHK3A_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO8739825.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(34554..35132)
                     /gene="wrbA"
                     /locus_tag="ACHK3A_001738"
     CDS             complement(34554..35132)
                     /gene="wrbA"
                     /locus_tag="ACHK3A_001738"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO8739826.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNS"
     gene            complement(35129..36439)
                     /locus_tag="ACHK3A_001739"
     CDS             complement(35129..36439)
                     /locus_tag="ACHK3A_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8739827.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYAERFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSQKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLGRQHQITVKLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNILNSRAPDVPQSDQEDEIKLYFSK"
     gene            complement(36492..36776)
                     /locus_tag="ACHK3A_001740"
     CDS             complement(36492..36776)
                     /locus_tag="ACHK3A_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO8739828.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            complement(36822..37073)
                     /locus_tag="ACHK3A_001741"
     CDS             complement(36822..37073)
                     /locus_tag="ACHK3A_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8739829.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIS
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            complement(37103..>37244)
                     /locus_tag="ACHK3A_001742"
     CDS             complement(37103..>37244)
                     /locus_tag="ACHK3A_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO8739830.1"
                     /translation="NIVGDLLRKSQKEARRAGRKQKRWEEACKALRELNKYRDLINEL
                     AE"
BASE COUNT         8824 a         9447 c         9771 g         9202 t
ORIGIN      
        1 cctgctgacc gcggagaaag cgcgagggat gaagggcgat gaaattaacg cctggcgtaa
       61 tgcgatggaa gcggcgaact acatgtttga gcacaatggg cgtaaatggg actacgggaa
      121 gtcaacgcag acgcgtcttg agccgtcggt ggcggcagcg aaagcgggga aactgccgga
      181 ggcgtttttc tggacggatg cggaaaacaa tgatgtggag gtgacagcag aagagcttat
      241 agcactgagt gaagcggcag agcaggcgat gttcaccaaa gggatggaaa tccacgtccg
      301 gcagcgcacc atgaagaaag agctggaaaa gctgaccagt gcggatgaga tactggcata
      361 cagggttggc tgggcacagg aataacagac aaaaagatgg gggaccatca cctcccccat
      421 gaactgagtc aattatacac ttgtgaagta tttactatgc gaataaatac tgtcacggaa
      481 agtgcaggcc tggatgtgat ggaaatcatg tttccggcgc atcctgttga tatggataat
      541 ttttcagatt tccggaagtc cgtattttgc ccgtgcgcgg ttacatgctt cattcgcgat
      601 gccattttcg ccggacattg cgaattcccg gcatgtggac ggacggttct tgtaaacaga
      661 gcaatatgcg tttttacccg gagttcccgc cagagcgaca catcggggat ttttctgact
      721 ggtgccgcgc atacagcgat gaaaggggga tatttgctca gtgagactga ccggaacctt
      781 tccgccagca tcgtcagctt ctgcccagta aaaagagacg cggaaaaatg cacaacaggc
      841 accacacgtc atgcatggat tggggttatt cataattcat cggtatagcg ataaatcgta
      901 aagagaacaa tgtacctagt gtgtattgag ctaaatatac caattagtat accaagtgta
      961 tactaagagc aaaaaaagaa aggggctacc atttggtaac cccttgattg tttggcggaa
     1021 gcgcagagat tcgaactctg gaaccctttc gggtcgccgg ttttcaagac cggtgccttc
     1081 aaccgctcgg ccacacttcc ggaatgacgc gcactataaa catcccgatg cggcgtgtaa
     1141 acccctaatt tgtttgtttg cctgaaaaac agccaaaagt gcattgatag catgaaataa
     1201 cagcagattg atcatttcat caccatgaat tccttctctt ttactcgttt agcaaccggc
     1261 taaacatccc cactgcccgg caaaaagaaa aataggtcca tttttatcgc taaaagataa
     1321 atccacacag tttgtattgt tttgtgcaaa agtttcacta cgctttatta acattacttt
     1381 ctggcgacgt gcgccagtgc agaaggatga gctttcgttt tcagcatctc acgtgaagcg
     1441 atggtttgcc ttgctacagg gacgtcgctt gccgaccata agcgcccggt gtcctgccgg
     1501 tgtcgcaagg agaagagacg tgcgatatga ataacgagga aacattttac caggccatgc
     1561 ggcgtcaggg cgttacccgg cgcagctttc tcaaatattg tagtctggct gccacgtcgc
     1621 tgggattagg cgcgggaatg gcaccaaaga ttgcctgggc gctggagaac aaaccgcgca
     1681 ttccggtggt atggatccac ggtctggaat gcacctgctg taccgaatct tttatccgct
     1741 ccgctcaccc actggcgaag gacgtcatcc tttccctgat ttccctcgat tatgacgata
     1801 ctttgatggc tgccgccgga acccaggcgg aagaagtctt tgaagacatc atcacgcaat
     1861 acaatggcaa atatatcctc gcagtagaag gtaatccgcc gctgggcgag caggggatgt
     1921 tctgtatcag cagcggacga ccgtttattg agaaactcaa acgtgccgct gccggagcca
     1981 gtgcgattat cgcctgggga acctgcgcgt cctggggctg cgtgcaggcc gcgcgaccca
     2041 atccgacgca ggcaacgcct atcgacaaag tcatcaccga caaacccatt atcaaagtac
     2101 ctggctgccc gccgatcccg gatgtgatga gcgccatcat tacttacatg gtgacctttg
     2161 atcgcttgcc agatgtcgac aaaatgggcc gtccactgat gttttatggt cagcgtatcc
     2221 acgataaatg ttatcgccgc gctcacttcg acgccggaga gttcgtccag agttgggatg
     2281 atgacgctgc ccgcaaaggt tactgcctgt acaaaatggg ctgcaaaggg cctaccacct
     2341 ataacgcctg ttcctccaca cgctggaacg atggcgtttc cttcccgatc cagtcaggtc
     2401 acggctgcct gggttgtgcg gaaaatggtt tctgggatcg cggttcgttc tacagccgcg
     2461 tggtcgatat tccgcaaatg ggtacccatt ccaccgccga taccgtcggt ttaaccgcac
     2521 ttggcgtggt ggcagcggct gttggtgtgc acgcagtcgc cagcgccgtt gaccagcgca
     2581 gacgccataa ccagcaacct acagaaaccg aacatcagcc aggcaatgag gataaacagg
     2641 catgagcact cagtacgaaa ctcagggata caccatcaat aatgccggac gccgcctggt
     2701 ggtcgacccg attacgcgca ttgaaggcca catgcgctgc gaagtgaata ttaacgatca
     2761 gaatgtgatc accaatgccg tctcctgcgg caccatgttt cgtgggctgg agatcatcct
     2821 gcaagggcgc gacccgcgcg atgcgtgggc gttcgttgaa cgtatctgcg gcgtctgtac
     2881 tggcgtacac gctctggctt cggtttacgc catcgaagat gccatcggta ttaaagtgcc
     2941 ggacaacgcc aatatcatcc gcaacattat gctggcaacg ctctggtgcc acgatcatct
     3001 ggtgcacttc tatcagcttg ccgggatgga ctggatcgat gtgttagatg cgctgaaagc
     3061 cgacccgcgg aaaacctccg aactggcgca aagcctctcc tcatggccga aatcatcccc
     3121 tggctatttc ttcgacgtac aaaaccgcct gaagaaattc gttgaaggcg ggcagttggg
     3181 gatcttccgc aatggctact gggggcaccc gcagtacaaa ctgccgccag aagccaacct
     3241 gatgggcttt gcccactatc ttgaagctct cgatttccaa cgtgaaattg tcaaaatcca
     3301 cgcggtcttt ggcggtaaaa acccgcatcc aaactggatt gtcggcggga tgccttgtgc
     3361 cattaacatt gacgaaagcg gcgcggtcgg ggcggtcaat atggaacgcc tgaacctggt
     3421 gcagtcaatc attacccgta cggcggattt cattaacaac gtgatgatcc ccgacgcctt
     3481 agccatcggt cagttcaaca agccgtggag cgaaatcggc actggccttt cggataaatg
     3541 cgttctcagt tacggcgcgt tcccggacat cgccaacgac tttggcgaga aaagtctact
     3601 gatgcctggc ggcgcggtga ttaacggcga cttcaacaat gtgctgccag tggatttggt
     3661 tgatccgcag caggtgcagg agtttgtcga tcacgcctgg tatcgttatc ccaacgatca
     3721 ggtcgggcgt catccgttcg atggcattac cgacccgtgg tacaaccccg gcgatgtcaa
     3781 aggcagcgat accaacattc agcagctgaa tgaacaggaa cgctactcgt ggatcaaagc
     3841 gccacgctgg cgcggtaatg cgatggaagt ggggccgctg gcacgcacgt taatcgctta
     3901 tcacaaaggc gatgcggcga ccgttgagtc ggtcgatcgc atgatgtcgg cgttgaacct
     3961 gccgctttcc ggcatccagt caacattagg ccgcattttg tgtcgcgcgc acgaagcgca
     4021 gtgggccgca ggcaagttgc aatatttctt cgacaagctg atgaccaatc tcaaaaacgg
     4081 caatctcgcc accgcctcca ctgagaagtg ggaaccagca acctggccga cagagtgccg
     4141 tggtgtcggc tttaccgaag cgccgcgcgg agcgttaggc cactgggccg ccattcgcga
     4201 tggcaagatt gatctctacc agtgcgtggt gccgaccacc tggaacgcca gcccgcgcga
     4261 tcctaaaggg caaattggcg cttatgaagc ggcgctgatg aacaccaaaa tggcgatccc
     4321 cgagcaaccg ctggagatcc tgcgtactct gcacagcttt gatccgtgcc tcgcctgttc
     4381 aacacacgtg ctgagcgacg acggtagcga gctgatctcc gtgcaggtgc gttaacagcg
     4441 aaggagaatc atcatgcaac agaaaagcga caacgttgtc agccactatg tctttgaagc
     4501 gccagtgcgt atctggcact ggctgacggt gttatgcatg gcggtgttga tggtcaccgg
     4561 ttactttatc ggcaagccgc taccttccgt cagcggcgag gcgacgtatc tgttctatat
     4621 gggctacatc aggttaattc acttcagcgc cgggatggtt tttaccgtgg tcttgctgat
     4681 gcggatctac tgggcttttg tcggcaatcg ctactcacgc gagctgttta tcgtgccagt
     4741 atggcgtaaa agctggtggc agggcgtgtg gtatgaaatc cgctggtatc tgtttctggc
     4801 aaaacgtccg agtgccgata taggtcataa ccccatcgcc caggcggcga tgttcggcta
     4861 tttcctgatg tcagttttta tgatcatcac cggctttgcg ctgtacagcg aacacagcca
     4921 gtacgctatt tttgcgccgt tccgttatgt ggtggaattt ttctactgga cgggcggcaa
     4981 ctcaatggac gttcacagct ggcatcggct ggggatgtgg ctgattggcg cgtttgtgat
     5041 cggtcatgtc tacatggcgc tgcgtgaaga tatcatgtcc gacgacacgg tgatctccac
     5101 catggtcaac ggctaccgta gccacaaatt tggcaaaata agtaacaagg agcgttcatg
     5161 agcgagcaac gcgtggtggt aatggggctg ggcaacctgc tgtgggccga tgaaggcttc
     5221 ggcgtgcggg tggcggaacg gctgtatgcc cattaccact ggcccgagta tgtggagatt
     5281 gtcgatggcg gtactcaggg actgaacttg ctggggtatg tcgaaagcgc cagtcacttg
     5341 ttgattctcg atgccattga ctacgggctg gaaccaggaa cgctgcgaac ctatgccgga
     5401 gaacgcattc cggcttatct cagcgcgaag aaaatgagcc tgcaccagaa cagcttctcc
     5461 gaagtgctgg cgctggcgga tatacgtggt catttgccag cacatattgc cctcgtcggt
     5521 ctgcaacccg caatgctcga cgactacggc ggtagcctga gcgaactggc gcgggagcaa
     5581 ctgcccgctg cggaacaatc ggcgctggcg cagctggctg cgtggggaat tgtgccgcaa
     5641 ccggctaagg aatcgcgctg tctcaattat gactgtctgt cgatggaaaa ttacgaaggc
     5701 gttcgcttgc gtcagtacag gatgaggctg gaggaacagg gatgagcaac gacacaccat
     5761 ttaatgcgtt gtggcaacga atgctggcgc gcggctggac gccggtcagt gaatgccgtc
     5821 ttgacgactg gcttacgcaa gcgccagacg gcgtggtgtt attaagtagt gacccgaaac
     5881 gcacgccaga agtcagcgat aacccggtaa tgattggcga attactgcgc gagtttcccg
     5941 actatacatg gcaggtagcg attgctgatc ttgagcagag cgaagccatc ggtgatcgtt
     6001 ttggcgtatt tcgttttccc gccacactgg tgtttaccgg tggtagctat cgcggcgtgc
     6061 tgaatggtat tcacccgtgg gcggaactga taaacctgat gcgtgggctt gtcgaaccac
     6121 aacaggagcg tgcctcatga gcgaaacttt tttccatctg ttggggccag gaacgcaacc
     6181 gaacgatgac agtttcagca tgaatccact gccgatcacc tgtcaggtga atgatgaacc
     6241 gagtatggcg gccctggagc aatgtgctca cagcccgcag gtgattgcgc tgttaaacga
     6301 gttacaacaa caaataagcg aacgccagcc accgttgggc gaggtgctgg cagtcgatct
     6361 gttaaatctc aacgccgacg atcgtcactt tatcaatacg cttctcgggg aaggggaagt
     6421 gtcagtgcgc atacagcagg ctgacgacag tgaaagtgaa atacaggagg cgatcttctg
     6481 cggattatgg cgggtgcgca gacgtcgcgg cgacaagttg ctggaggaca aactggaggc
     6541 tggctgcgcg ccgctggcat tgtggcaggc ggcaacgcaa aacgtcttgc cgacagattc
     6601 gctgttaccg ccgcccattg atggcctgat gaatggccta ccgttggcgc atgagctact
     6661 ggcgcatgta cgtaaccccg acgcgcagcc gcacagcatt aatctgacgc aattacccat
     6721 cagcgaggct gatcggcttt ttctctcacg tctctgtggg ccgggaaata ttcagattcg
     6781 caccattggc tatggcgaga gctatatcaa cgccacgggg ttacgccatg tctggcattt
     6841 acgctgcacg gacaccttaa aaggcccgtt actggaaagt tatgaaatct gcccaatacc
     6901 ggaagtggtg ctggcagcgc cagaagattt ggtcgactct gcgcagcggc ttagcgaggt
     6961 atgccagtgg ctggcggaag gtgcaccgac gtaaaaagac ggtaagtatc gctttcaggc
     7021 ttatgaatat cgcaatcggc gaatacctct ggtcgtagag tttcaggata aagagggaga
     7081 tctaccatta acgggttatt tttctctctt cgcctacagg agtgcgcatg tgggatgtca
     7141 ttgatttatc gcgctggcag tttgctctga ccgcgctgta tcacttttta tttgtacccc
     7201 ttaccctggg gctgattttt ttgctggcta tcatggaaac catttacgtg gtcaccggca
     7261 aaacaatcta ccgcgatatg acgcgcttct ggggtaagct cttcggtatc aactttgctc
     7321 ttggcgtggc taccggcctg acgatggagt ttcagtttgg tactaactgg tcattctatt
     7381 ccaactatgt gggcgatatt tttggcgcac cgctggcgat ggaagcatta atggccttct
     7441 tcctcgaatc cacctttgtc gggctgttct tcttcggctg gcagcggctg aataaatacc
     7501 agcacctgct ggtgacgtgg ctggtggcgt tcggttcaaa tctctctgca ttgtggatat
     7561 tgaatgccaa cggttggatg caatacccga ccggtgcgca ttttgatatc gacaccctgc
     7621 gtatggagat gaccagtttc agcgagttgg tctttaaccc ggtcagccag gtgaaatttg
     7681 tgcacactgt aatggcgggc tacgtgaccg gggccatgtt tattatggcg atcagcgcct
     7741 ggtatttact gcgcggacgg gagcgcgatg ttgcattacg ctcgtttgcc atcggttccg
     7801 tcttcggtac tctggcgatt atcggcacgc tgcaactcgg agacagttct gcgtatgaag
     7861 tcgcgcaagt acaaccggta aaactggcgg cgatggaagg ggagtggcaa acggaacctg
     7921 cacctgcacc gttccatgtg gttgcctggc cggaacagga tcaagagcgt aacgcctttg
     7981 ctatcaaaat tcccgcgctg ctagggatcc tcgccactca ctcattagat aaacccgtgc
     8041 cgggtctgaa gaatttgatg gctgaaacct acccacgctt gcaacgcgga cgtatggcct
     8101 ggctgttaat gcaggaaata tcgcaaggca atcgtgagcc gcatgtgttg caggcatttc
     8161 gggagctgga aggcgacctt ggttacggca tgttgctctc ccgctatgcg ccggatatga
     8221 accatgtcac agctgcacag taccaggcgg cgatgcgtgg cgcgatacct caggttgcgc
     8281 cggtattctg gagtttccgc atcatggtgg gctgtggttc tctgctgcta ctggtgatgc
     8341 tgattgcgct tgtccagacg ctgcgtggca aaattgacca gcatcgctgg gtgctgaaaa
     8401 tggcgctctg gagtttgccg ctgccgtgga ttgcgattga agccgggtgg tttatgaccg
     8461 agtttggtcg tcagccgtgg gcgatacagg acatcttacc gacatactcc gcgcactccg
     8521 ccttaaccac aggacaactg gctttctcac tgatcatgat cgtagggctt tacaccctgt
     8581 tcttaatcgc cgaagtctac ctgatgcaga aatatgcccg tctggggccg agcgcgatgc
     8641 agagtgaaca accgacgcag caacaggggt aaaggagaaa atcatgtttg attatgaaac
     8701 attgcgcttc atctggtggc tgctgattgg cgtgatcctg gtggtcttta tgatctccga
     8761 cggatttgac atggggatcg gctgtctgct gccgctggtg gcgcgtaatg atgatgaacg
     8821 tcggatagtg ataaacagcg ttggcgcgca ctgggaaggc aatcaggtct ggttgatcct
     8881 cgcaggtggg gcattatttg ccgcctggcc cagagtgtat gcagcggcgt tttccggctt
     8941 ttatgtggcg atgattctgg tgctgtgctc gctgttcttc cgcccgctgg cctttgatta
     9001 tcgcggaaaa atcgccgatg cacgctggcg taaaatgtgg gatgccggac tggtcatcgg
     9061 cagtctggtg ccgccggtag tcttcggtat tgcattcggc aacttgctgc tcggcgtgcc
     9121 gtttgctttc acaccgcaat tacgcgtgga atatctcggc agcttctggc aactgctgac
     9181 gccattccct ttattgtgtg gattgctcag tcttgggatg gtgattttgc aaggtggcgt
     9241 ctggttacaa ctgaaaactg ttggtgtgat tcatctgcgt tcacagctgg cgaccaaacg
     9301 cgccgcacta ctggtgatgc tgtgcttttt gctggcgggt tactggctgt gggttggtat
     9361 tgatggcttt gtactgctcg cccaggatgc taacggtcct tccaatccgt taatgaaact
     9421 ggtggcagtg ctacctggtg cctggatgaa taatttcgtc gaatcgcccg ttttgtggat
     9481 cttcccgctg ctggggttct tctgcccatt gctgacggtg atggcgattt atcgtggtcg
     9541 cccgggttgg ggatttttga tggcgtcatt gatgcaattt ggcgtgattt tcacggcagg
     9601 catcacgctg ttcccctttg tcatgccgtc aagcgtgagt ccgatctcca gcctgacgtt
     9661 gtgggacagc acttccagtc agctgacgct gagcattatg ttggtaatcg tgctgatatt
     9721 tttgcctatt gtgttgctct acactctctg gagctactac aaaatgtggg gacgcatgac
     9781 gacagaaact ctccgccgta acgaaaacga gctgtactaa ggagcaggaa caatgtggta
     9841 tttactttgg ttcgtcggca ttttgttgat gtgttcgctc tccacccttg tgttggtatg
     9901 gctggacccg cgtctgaaaa gttaacgaac gtaagcctga tgtggtgcat tagcatcgca
     9961 tcaggcaatc aataatgtca gatatgaaaa gcggaaacat atcgatgaaa gcgatcttaa
    10021 tcccattttt atctcttctg attccgttaa ccccgcaatc tgcattcgct cagagtgagc
    10081 cggagctgaa gctggaaagt gtggtgattg tcagtcgtca tggtgtgcgt gctccaacca
    10141 aggccacgca actgatgcag gatgtcaccc cagacgcatg gccaacctgg ccggtaaaac
    10201 tgggttggct gacaccgcgc ggtggtgagc taatcgctta tcttggacat taccaacgcc
    10261 agtgtctggt ggctgacgga ttgctggcga aaaagggctg cccgcagcct ggtcaggtcg
    10321 cgattattgc tgatgtcgac gagcgtaccc gtaaaacagg cgaagccttc gccgccgggc
    10381 tggcacctga ctgtgcaata accgtacata cccaggcaga tacgtccagt cccgatccgt
    10441 tatttaatcc cctaaaaact ggcgtttgcc aactggataa tgcgaacgtg actgacgcga
    10501 tcctcagcag ggcaggaggg tcaattgctg actttaccgg gcatcggcaa acggcgtttc
    10561 gcgaactgga acgggtgctt aatttttcgc aatcaaactt gtgccttaac cgtgagaaac
    10621 aggacgaaag ctgttcatta acgcaggcat taccatcgga actcaaggtg agcgccgaca
    10681 atgtctcatt aaccggtgcg gtaagcctcg catcaatgct gacggagata tttctcctgc
    10741 aacaggcaca aggaatgccg gagccggggt ggggaaggat cactgattca caccagtgga
    10801 acaccttgct aagtttgcat aacgcgcaat tttatttact acaacgcacg ccagaggttg
    10861 cccgcagtcg cgccaccccg ttattggatt tgatcatggc agcgttgacg ccccatccac
    10921 cgcaaaaaca ggcgtatggt gtgacattac ccacttcagt gctgtttatt gccggacacg
    10981 atactaatct ggcaaatctc ggcggcgcac tggagctcaa ctggacgctt ccaggtcagc
    11041 cggataacac accgccaggt ggtgaactgg tgtttgaacg ctggcgtcgg ctaagcgata
    11101 acagccagtg gattcaggtt tcgctggtct tccagacttt acagcagatg cgtgataaaa
    11161 cgccgctgtc attaaatacg ccgcccggag aggtgaaact gaccctggca ggatgtgaag
    11221 agcgaaatgc gcagggcatg tgttcgttgg caggttttac gcaaatcgtg aatgaagcac
    11281 gcataccggc gtgcagtttg taatgcataa aaaagagcat tcagttatct gaatgctctg
    11341 aggctgatga caaacgaaga actgtctaat gcgtagaccg gaaaaggcgt tcacgcccca
    11401 tccggccacc ttaccttcag ttttactctt tctcggagta actataaccg taatagttat
    11461 agccgtaact gtaagcggtg ctggcgcgtt taatcacacc attgaggatt gcgcctttaa
    11521 tattaacgcc agcctgttcc agacgctgca ttgacaagct cacctctttg gcggtgttca
    11581 agccaaaacg cgcaaccagc aggctggtgc cgacagagcg tcccacgacc gcagcatcgc
    11641 tcaccgccag catcggcggc gtatcgacaa tcaccagatc gtaatggtcg ttcgcccatt
    11701 ccagtaattg acgcatccga tcgcgcatca gcagttcaga cgggttaggt ggcacctgac
    11761 cgcgagtaat cacatcaaag cctcctttgc caaaatgctg gatcactttg ttgagctcat
    11821 ctttacctgc cagatattcc gacaagccat gttcattact cacggtaaac aggttatgcg
    11881 aataaccacg gcgtaagtcg gcatcaataa ataacacttt ttgatcggac tgggcgatca
    11941 ccgctgccag agttgaactg acgaacgttt taccactgtc tggcgtcgca ccggtgatca
    12001 tcagaatgtt attctctgtc tccatcatag cgaagtgcag actggttcgt agcgcacgta
    12061 cggcctccac agcagaatcc gccgggttat ccaccgccag gaaggggata tttttagtac
    12121 gatggcgctg ctgattagaa aataaatttt tcttacgcag acgggtacgt ttatccagcc
    12181 actcggacat tgggatagtg gcataaacgc tgatgccgtg ctcttccagt tgttccgggg
    12241 cttctacacc acgacgcaac atcgcacgcg ccagcacggc acccaccgaa ataaacaggc
    12301 caagaataaa accaagcacc acattcaacg cttttttcgg tttcactggc tgcggctgag
    12361 tgactgccgg gtcgataatc cgcacgttac caatcgcact ggatttcgaa atactcaact
    12421 cctgctggcg gttaagtaat tgcagatata ccgcacggcc cgcttctacg tcacgactta
    12481 aacgcaacac ttcctgttgg gtggaaggca ttgccgatac ccgcttattc aggcgtttgc
    12541 gttcttgctc cagtgtctgg cgtttttcca gcagcgcacg ataagttggg tgatctttct
    12601 tatacagctg ggagatctct gcctcgcgga aagtcagctc attgagttga ttatcaacgt
    12661 tcacaatctg ctcaagaacg gctttggctt ccaggttaag gtcaaccgaa tcgcgctgtt
    12721 ggcgataaac gttgagtttt tcttccgcct ggtccagctc gctgcgcact tctggcaact
    12781 ggcgctgtaa gaattcaagg ctttgtgaat cctgcgccgc ctggcgagcg atattctgtt
    12841 gcaaatagtt gttagcgatg ctgttcagaa tacgagtaat caactgggga tcatcaccgg
    12901 tcatggtaag ttccagcatc ccgctttctt tactgcgttc gctaacggta aaggtttcct
    12961 gcaacgcgtt aatcgcttcc agttcggtac gctggctcag gacaaactgc gttcctggtt
    13021 tggccttaat gtccgcgata gtcagcgcaa cgccgtcttt ttccagacgc tggccgacca
    13081 taccattgac ggtgaaccct tcaccttcca gcgtatagtg gccgttttcc ccaactgtga
    13141 gtgtcagttg ctgatcctga ccattcagtt gtgggatgtg catccagctg atcgccagct
    13201 cacctggttt ctctttggtt aatctcgccc agccgcgacc cacaatcgga aaatacttct
    13261 gctcaaccat gtcgcgcaga ttcagttcag caatggtttt accgagaatc atgcgcgatt
    13321 gcagcagttg gatctccggt gctgactcgg gcgatgagtt agggatcata tcgctcaggc
    13381 cgctgagaat ggcgttgccc tgtttctgct caacctggac cagagtatct gcctgataaa
    13441 ttggcgtgct taacagcgag taagcgacag cgatcagcgt gaacaacgcg gtcacgctga
    13501 caataaactt acggtgatcc cataactcgc cgaccagacg aagcagatcg atctcatttt
    13561 cctgagtgct gcctggtggc gtattcatat ttttagttgt catcgtattt tataaactca
    13621 aattaccggc tgaggcgctt cgcccattcc tgactggcgc gctccaacat accgtagaca
    13681 tgctcaaatg cgtcctgact tttacgatag ggatccggga tctctttctg ttccagccat
    13741 tgcccaaaca gcattgtctt cccgcggacc tcgggagcga ttgccgtaac ctgagcaata
    13801 tgttccgact ccattgccag aatcagatcg taatttcgtg ccatctcagc agtgagctta
    13861 cgtccggcat gtccttcaag agatacgcca tgattagcgg caacatctgc cgctgtcgca
    13921 tctgcagggt gctttaccag accatgaaca cctgccgatt tgacttttac gcctggcaga
    13981 cgtttacgca gcaagcgctc gccaatgggc gaacggcaaa tgttgccggt acaaaccacc
    14041 aggattgagt taaattttag ttgggccatg tttgaatcca ccgtgaagtc tctgtcaggt
    14101 catgcacccc ggaaatcgtc ggtaccagta gagaaatcac acggttccaa cgagccagcg
    14161 gcgcagtagt gacataaaca atgtcgtaag gttcaagctg gaattccgtt ccgagtatca
    14221 ttgccgatgc gtccttcgca ttcagctgat aaatattggc gattttgcca ttctgcttgt
    14281 tttgcgttgc acgaataaca aaaatccccg tcgcatcagc gacatcctga ttcataccct
    14341 cagcgttacc cagcgcttcc gccagggtca taccgctgcg gtccattttc agcgtgctct
    14401 gtttacccac ttcacccatc acgaagactt tcagatcatc gttgcgcgga ataaataaaa
    14461 tatcgcccgg gtgcagcagt ttgttctggc gcaagtcgcc acgctgcatc agcgcataga
    14521 ggttcacttt ggtctttacg ccattctggg tgagcaccac gttgcgccag tctgcatctg
    14581 cggtcagccc ccccgcggcg ttaatcgcat ccatgatggt cagaggaata ttggtaatcg
    14641 gctgctgacc cgatttgctg acttcaccgg tgacgtacgc tttttgcgag cggaacgccg
    14701 cgacgctgac atcgacctgc gggctttcaa tgactgaatc cagacgcgct gtaatctcgt
    14761 tgcgtacctg ggtaagcgtt ttcccggcca ctttcaggcg accaatatag gggtagaaaa
    14821 ttgcaccatc agcgttaacc cagttgccgg tatcactggc gctacggtat tgccctgcgg
    14881 gtgtggttag ttccgggtga tcccagaccg taaccatcaa cacatcacca atgccgatac
    14941 gatattcata gttcgcgatt tgctgctcca gctccgggtt agcctgcgac ataatcggtt
    15001 tggcgcgcag ctgatcaatc aatcctggcg tgacgggata gatattgacc attttgtcca
    15061 ggtcatactg attgtcaggt agttcaatga catccttgtt actggtcgat aaattctgac
    15121 caggaacaag ggtacatgct gtcaaagaca gaacagccaa tgtcagtacc gaaaacttaa
    15181 aaatgttctt tttcattggt ttatatttat cagtcattgt aatgttatga attacgtcag
    15241 gttattgtcc aaccggtact tctcgctctg ccgtcattgg atagagctgg tatttgcgtg
    15301 acagcatttg tccaccatca cgcgtcaatg gtgtccacga aaccaccgcg cggttgcggt
    15361 taggtccaat ggtcatcaag tcgaacggaa tcgagatata aaaaccttta ctaaagccgc
    15421 cttcgccgta gtcatctttt gatacgttac tgattgccgc ccatacccct actgccacgc
    15481 cgctgtcaaa gcgtttggcg acgtcgatcg ttgcaccttt atcttttgcc agatattgcc
    15541 caacgctaag tttcatcagt acgccattga gcgtcggcgg gttccagtaa gccgtcacga
    15601 aacctgttgg cgtagaataa tcggtgaagc gcatcatgtt gtcccagtca cgctgcttca
    15661 cgtagttaac gtccacaccc agcgcccagc tggcatctag cgggcgataa agcagctcgg
    15721 aaccgacacc ggcatacatc gtttccagat aaccgccata cacctggcca tagaaaccgt
    15781 tgcctaagtc ggcaaagtag ttcgcttgca agttgttgag ataaacgtca ttgcgaacgt
    15841 aatcacggat atgcgtgcgc acgcggggca gggtagagtc cgcgggtaat agcgaagact
    15901 taaacttgtc gtagttattg taaatattgg tgaaaatacc gccatcaagc agcaggtggt
    15961 cggtaaacca gtagcgggcg ctggacatca gccccagctg gaacatatag aaatcttccg
    16021 ggccgcccag cgactgtgaa agtgttgggt tgaaagagta gctaaagcga tcttcacgaa
    16081 tacggtagcc ccgaccaaag gcagaaagat cttcagcttc aacacgttgt tgttgcagtg
    16141 gctctgattg accaggcgct gtaccggcca gttgtttgcg caggctggct acgtcggttt
    16201 cggtagtcac catcgccata tgctcgcgct tttgcgtcac gctaatcttc tcaacgcctt
    16261 gcggcaggtt attcaccaga atccggttgg cacgatcgac cgcttcgcga gagtcacggt
    16321 atttgtattg ctgaccagac atatacagcg tcttatcgcg tagctgaatt tctggcgcgt
    16381 caaagcccgc gttatacttc agggcggtaa gttggtttgc taccgtggtg tactgcaatc
    16441 cttcagattc cggcgcaggt tgatatgccg gttttggcgt atcgcgcagg gcagggcgca
    16501 gatcgttgaa attggtccgt aacgtgaagc caaacatcaa cgtgttaccg cgttcataac
    16561 tcaggttgag atctgcccag ctggcagcgc gatagactgc gccgacgttg aaatggcttg
    16621 cctgaggcag tttgcccgca aaatcattct ggtaattgtt gccatcgtat tcgagtttca
    16681 gacgcagagg attccacggc gtttgatact caataccgcc aaaaatagag gctggaccac
    16741 gaaagatatc gctaaagctg atatctcccg catcgtgaga ctctgcgcga tggcaatatt
    16801 tatcgcttac ccgacaaaac gggttggtaa tattgcccgc attaccggcg tagccccagg
    16861 ccatcccgag ggtgaaatcg aacgggcccg cttgcttgct ggccaccaaa tattcaccgt
    16921 caaacagacc cgtaccagca atatcacgtt taccaaacgc cacttgcggt agccagtaac
    16981 cttcttccca caggcgaagt ttaaaatcga aagatttgtc tttgtatgac tgatcgccgc
    17041 tgaaatcttc ccactggcta tatttgcgtg tgcgcacatc cgtataacga atggtgcctt
    17101 ccagccatgg gaacagcgcc acggaggtgg agtagaaacg gtattgatcg ttatcccggt
    17161 agtttacgct gaattcacct tccggtgcga tgcgcgcatt tggcatctgc aacaatcctg
    17221 tgccgccaaa gtctgattgc gacgaaccca gcggatccgg ataggttaat acttcagcat
    17281 gacaggcact ggagacggca atggccaggc agcttaaaag ataagagttc ttcttcatca
    17341 gtcaggaaca cgttgcgtta aaacagacac aatctggtca tttaaatcag catatttttc
    17401 gggcagtaca tgcgcagaaa agcctaacca aagctggctg ccaggcggtg gttccacatg
    17461 tcttttattc cacagggcaa caggggcgac gacagcctca ccctcaggcg taatcaccat
    17521 aacgttattc ttgtccgcgc ctgcaagacg ggtgtgatct tgcagataat cggtaacgct
    17581 acggcctgcc agccagggca attgcccagc cccagaaacc gcacctagca gagtaatagt
    17641 caccggacgc tgtacggtat agagcgtata gtccccgacc agcgggggat tgctgttctc
    17701 atccactcgt acaaaatcag ggtccagttt gaccggtaag cgaccagtga tattgagatt
    17761 gagtaactgc tggcgcacgg atttaatcgt tgccaccaca tcgtcgtcag cttctgcttc
    17821 ccaggaagca agctgtgcca tgacatgttg gtagtcttta agcgctttag ctttcgccgc
    17881 agagtcggtc agcaaggcac caggccacca gaggcgatcg cgcagttgcg gttgtgtcac
    17941 caactggaca acattttcca cggggccaac ggaaagtgtc tgttgttcgc caggcagata
    18001 aatagtcacc gttccttgcg caaaggcatg gggtgtcatt acgtaaagta cgctggcaat
    18061 gaaatacgac tgtaatttat tcatggtgcg ggtttaagaa acgtcatttc tacgggaata
    18121 acccctgcgc ctaacatttg ccgactttga cgaacctggc cagtagccga atcgacccag
    18181 aatgtgtttt gccactgttt ttctggcagc gtggaagtca cctcttcctg ccagacatta
    18241 cacagcacat cgcgacccgc aatattcagc gtctctaatc cagcaaagga aaaagtggaa
    18301 ctcagggtcg cagagcgaaa atggttgtct tcactccaga gaatatcgcg cgtccagcgg
    18361 ctgccgtctt ttattgccag cgcgttacgc agagggtcct gcccggaatt tgtcacttcc
    18421 agcagattat tattaagttt gacggttttt aaaagacgtc cattgtgggt aaccagcatg
    18481 gcgttatcct gggataacca tttgctttgt tcttgttcta tatatcccag taccacaaag
    18541 attcgttgcc cttcattaag gcgtaaatac atcgtggaat aaggcaacgc ctggatctgc
    18601 tgatccgcta cggtgatatc ctgattatcg aaaaggctgg cgcgaaatgt atcgaccata
    18661 ctttgctggc tgtgcgtaca tcccgcaaga aatagtgcga aaatcgataa aataagaggg
    18721 cgcaccttta atccttgaaa ggttaattaa aaaaatgcaa atctctaaag cccggtacga
    18781 atatcgcaac cgggtatcat ttaactttaa ttttgacgcg ttaaagcagt attagcgcgt
    18841 agtggacgtg gtggttgagg tgttatgaga accgccatcg ccactgtcat ttgctgcaac
    18901 tactgccaac gtcgccacac caacagccgt tgccacaccg acaaccgtcg ccgtgccgac
    18961 agcgccagca gccacgccac cagtggtagc agcagtcgtg gttgcacctg tagtggtcgt
    19021 tgtggtgcca gtagtacccg tcgttgcctc agtcccgttt gctgcttcag gggcagcaaa
    19081 tgcagctggc gtcgtcaaca tgaaggccat aaggattgca gaaagcttgt gtttcataac
    19141 ttttccttta ttcatcgcat ggacaatacg tacgcttgag atcaggatta tctactttga
    19201 gtataacatc cttgtgaaat ctccctttcc tcataccgcc agcactatat actgcgggtg
    19261 ggaaaggcgc gcagagggaa atataagatt gttaactatt cccctctgga aatacgactt
    19321 gtgagcataa gataaatctt agcaaaatac aaattaaaga taagaaaatt gttaagaaag
    19381 ctattaacta ttgttggtaa tatagtttca agtggtacta taactggctg ttaagtggca
    19441 tttataacta ttaggtgcag agatattcgc ttaaatggat ttgttgctcg gtgaaaaagt
    19501 aacttgcgtt ataatttatg gtgttgacta ttgagctatt ccaataaaac cagccaactt
    19561 taaacaagca actcatatta agagagataa acatttgccg ctgttggtcc tcgcaggcca
    19621 tttacgcggc aaaattccac gcgtaatcct ggtataagca cttctgcgtc gcggggagtg
    19681 aatgcggaaa tatggacctg gacttcttta cgaccgtcgg aggggataat gaatcctttg
    19741 ccgcttttgc gatcaaaggt tttgacaatt cctgtcattt tacgggacaa acaaattcct
    19801 tactgaaaat actgcgctgc actatacggg gttaataaaa taaagccagc gatatttaag
    19861 accgccggac ggctaaaata aaatttgctt aatctcaatt atcatgcgtt aatagctgcg
    19921 tcggtttgaa agacagacag catacaaagt agtttactaa agcagttctc attatcaggc
    19981 attatcccct tcttttgagt ctctctcctg aacactaagt agtttctgta ctaaagccct
    20041 gtttgccgaa aggcccaaaa tgaaggaagt aaaatatgtc taataaaatg actggtttag
    20101 taaaatggtt taacgcagat aaaggttttg gctttatcac tcctgatgat ggcagcaaag
    20161 acgttttcgt ccatttcacc gccatccaga gcaatgaatt ccgcacgctg aacgaaaatc
    20221 agaaagttga attttctatt gagcaggggc aacgtggtcc cgcggcagcg aacgttgtta
    20281 cgctctaagg ttgccgttat tactccacat ctccatttcc gctgtccatg ttgtcatggt
    20341 tcacagtacc gcacatcggc attcgatgtg acggagcgaa atcctttagg ggcaaagtgt
    20401 attttttgta aatcgacgat gatcaccttt gataacgtcg cgctgcaaat acgcactgac
    20461 catgcgccgc tggatttcac aaaataatat caggctccct cgtggagcct tttttatatc
    20521 tgccttattt ttcttcaacg ctgtatgtat agtaagcgat aacctgttga ttattgaatc
    20581 tttcggggag atggcttata acatttctta cctgaccagg gtaccgggaa ccaacacctt
    20641 actggcgtgt tgctgtcttt caagaccaga agaggttaac agtgaatatt gaagagttaa
    20701 aaaaacaagc cgaaacggaa atcgctgact ttatcgcgca aaaaatcgcc gagctgaaca
    20761 agaatacagg gaaagaagtc tctgaaattc gcttcaccgc acgagaaaaa atgaccgggc
    20821 ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg tgaacatcac ccgtgcgagg
    20881 tgatgttccg cttgttgcta atttagtggc caatcattgg cgcttgtgga attaagcgtc
    20941 ggtacaattc ctccggcacc gggctttgcc atactcccgc atacattgcg taaccaatca
    21001 ccgcaaacat aatccccaga atcagtagcg tcattaacca gccagacaac gcaaaggctt
    21061 ttttatttgc cgcaggtttt tgcagtgaaa aggtcaatgt tgaggctacc ggacatgact
    21121 ctacgcaagt catacagccg gtacattcca ctgttcgtac ctgaattaat ttatcgaccg
    21181 ggatccgtga tgggcaattt tttgcgcatt tgccacagtc gatacaactt tcggcattgc
    21241 gacgaatctt aaacggcgat aatagcgaaa ccacgcccat cagcgcgcca tatgggcaaa
    21301 gataacgaca ccaggcatgg cgaataaaca ggctggcaat cagcaaaacg atcatgctga
    21361 ttaatgtcgc ggtccccata tgacgaaaga aattgagcat tttaacgtcc atcaccacgc
    21421 tgtagggcga caacataaaa tagtgaatcg cctgagcggg catcaataac gcaatataga
    21481 gaaaaaaact caacagcaaa tacttcacgc cgcgcagagg agtatccagc cagcggggaa
    21541 ggacaaattg ccgaccaaac agtttgttac cgagatcgcc gattaattca gaaagcgtac
    21601 caaccgggca taaccatgaa caaaaggcct tttttaataa tagactgatg acgataaaag
    21661 cgaccaataa cagcatcgcg gcggcgtgga cggacggtaa ctggcctgtt gcaaggctat
    21721 atttcagatt catcagaccg gcaatcggta gccagccttc gatacctccc ggtctggtga
    21781 caaatgtcgt actacctgcc gtttcgtaat agcgcaccca ataccagaac gtgatggcaa
    21841 tataaatatt cattgccaac agtaataatt gcgtcgcttt acgccaggtc gtggcattgc
    21901 gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg ccgccgctgc cagcgggttc
    21961 ttcttttctc tgtcatgatt ttgccagtcc gttaagttgc ataccaaatg ccactattct
    22021 agttgttctt aactggctga tattgattca aatcgcgttc aggtctttct tatgcaacca
    22081 tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt ggaagggggg cagataatgg
    22141 ctgctgctcc agttgtgcgc atagctggct ggcgatacgc attcccaggc tcgagcaact
    22201 gctttttagc tgatgtgcgg cacgctttat tttctcgcta tcctgattgg cgcgggcaat
    22261 gtcgatgtca tcgagaagcg gcagggcatg ttgtgtaaat aataccagcc attcgtggat
    22321 cttctccgtc cccattaact gagcatcttc attgagttgc gatacatcca gcggttgatc
    22381 gttattgact tgcagttgga gatagtgcgc cagtaactga ccgagcactt cacgcggcac
    22441 cggtttaggg ataatcccgc gaaatagcga actggtacgc tggcgcaacg tttcgtcgat
    22501 gacatgggcg ctaaagccaa tcaaaaccag cgacggatat tgctgtgcca gttgccgggc
    22561 aagcgtaatg ccgtcgatat ccggcagatc aaaatccacc agtgcgacag caaatggttc
    22621 actattttgc agtgtctcta aagcctgcgc ggcatttcct acggcaacga cctgcgcccc
    22681 actgctgttc aacatctcaa cggtaatccg ctgggttagc gggttatctt caattaacag
    22741 caaacgtaaa ccgtccagac gcaccgcctg attgactgtt tttggcacgg gtgcagtggc
    22801 aatacgtaac agcaagcgta aacaaaaaca gcttccaacc tccggcgtgc tggtggcgct
    22861 cagttcgcca cccatcgcct gggccagacg gctactgata gtcagcccca gcccggtgcc
    22921 gccgcgtttg ccgctaacct gaacaaatgg ctggaagatt tcagccagtt tcgcgggatc
    22981 aataccgcag ccgctgtctt ccacttcgac cagccattgt tcgccatcag tgcgactacg
    23041 caggataatg tgcccttcgt cggtgaaacg cagggcgttg ctcaacaggt tggtaatgac
    23101 ctgacgaata cgctgcggat cgcccattaa cgcagtcggt acatcgtcgg caattgccgt
    23161 tgccaggcga atcgggcgac ctttcacccg tccgctcatt aattgcaggg tactttccag
    23221 caacgggcgc ggttcaaagg gttcatcgct gaccgaaaca ttcttgccgc ctgcttcgat
    23281 agccgaataa tcgagaatat cgttgaggat ggtcagcaac gattcgccag agtcggtaat
    23341 tgcccgcaaa tcatcacgct gagcgttaag tgcggggtta tctgccagta gctgtgcggt
    23401 accgagaata ccgtacagcg gtgtgcggat ctcatggctc atcgccgcca gaaacgctga
    23461 ttttgcctgg ttggcttttt ctgcttccgc ccgtgcctgt cggtgttcta tcaccagttc
    23521 ctgcaattca gctgtacgcg ctttgacctg cgccgccaac tgttcacggt ggcgattcag
    23581 tgcatgaaca ctgctgcgaa acgcatccat cagccgcccg atggtgtcca gttcccgcac
    23641 gccagcggtt tccgggaaag gggagtcgat atcaccatcc agcaagcgtt gtagcgcctg
    23701 cgtttgttct gcaagagggc gcgtgactga gcgataaacc acgcgccaga ggatcagaat
    23761 cagtgcgcaa agagaaacta ttcccagcaa taacaggctg tattgcccgc gtgcactggc
    23821 tttttccaga tgtgccagcc cgtgctgatt acgcagctca atggtgtcga ccagctgact
    23881 gacttcgcta ctaaactgcg cgaattgggc gatgttattt tgggcgagag tttgcaggtg
    23941 attgctgatt tcactgtcct gctgatacag cgccagcaaa tcgctatatt ggctaacggt
    24001 agttaacgtt gttgctacct gagtacgaac acccggatct tcaatgcgta tttgccgacg
    24061 ttgcagaatt ttcaccgcat tattgagctg cttttccagc gttggcgcat ttttctggat
    24121 ctgttccagc cccagattca tcaccatttg ctgcacccgc agggcgctaa ggcgcagttc
    24181 attcatctgg ttaacatact caagatcgat atcaatcagc cgatcgagtg cactttctgc
    24241 ggcctgacgc tgatgttgtt cgatcaaatc gtaaatcccg gcctgggtcg ctccggcgga
    24301 ggttgccgca ttattcgcct gaccttgcgc caggcgtgcg atttcatcgg cggcggcgac
    24361 tatctgctga ctgagttgct gttgttgctg gcgaagttgc aaacgctgcc ccaccagttc
    24421 cccttgctga cgtaacgaac gggagatctc ctgctcctgt tgttcaatag cggtggtgtc
    24481 aaaatcttgt tcccgtaacg cttgcagcaa cgcattaatc ttcaggcttt gtgcggtgag
    24541 cattcgcccc tgcgcctgcc acatcttttc gttatcggca ctggtcaggt tctgcgcggc
    24601 gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc atattcatgg taggaatcaa
    24661 tgcctgagtg ttgtcttttt ccacctggct gataaagcgc aggttgtacc atcccaccag
    24721 ggtactggtc agggttaaca gcgccatcag ggcaaagccc atccagagtc ttcgggtcag
    24781 ggttaaattc acggtcggtg cactttgggt gaaaaaggtt gagtcgcaaa gcggaatgca
    24841 tctagcataa agccttatta ttgatgaggc tatcatgcgc gtactgctat tgttactact
    24901 ttcccttttc atgttatcgg cattttcggc tgataacctg ttgcgctggc atgatgcaca
    24961 gcattacacg gtgcaagcct ctacgccgct aaaagccaaa cgcgcatgga aactgtgcgc
    25021 gctttatccc agtttgaaag attcatactg gttatcgttg aactatggta tgcaggaggc
    25081 tgctcgccgc tacggtgtgg atttaaaagt gctggaggca ggcggctaca gccagttggc
    25141 tacccagcaa gcacaaatcg accagtgtaa acagtggggc gcagaggcca ttttgctcgg
    25201 tagtagcacg acgtcatttc ccgatctgca aaagcaggta gcaaatctgc cggtgatcga
    25261 actggtaaat gctattgatg ctccccaggt gaaaagccgc gttggtgtgc cctggtttca
    25321 gatgggctat caacctgggc gatatctggt gcaatgggcg cacggtaacc cactgaacgt
    25381 gctgttgatg cccggacccg ataacgccgg gggcagtaaa gagatggccg agggttttcg
    25441 cgcagctatt gctggaagcc cggtacgtat tgttgatatt gcgcttggcg ataacgatat
    25501 tgaaatccag cgtaacctgt tgcaggagat gctggagcgc catccagaaa tcgacgtcgt
    25561 tgccggaacg gccattgcgg cagaggcggc aatgggtgaa gggcgtaacc tgaaaacgcc
    25621 gcttaccgtg gtgtcgtttt atctttcaca tcaggtgtat cgcgggctga agcggggaag
    25681 agtgattatg gctgccagcg atcagatggt ctggcagggg gaactggcgg ttgagcaggc
    25741 catcaggcaa ttacaggagc aatcggtgtc tgataatgtc agcccaccga ttttagttct
    25801 gacgccgaaa aatgccgacc gcgaacatat ccgccgctcg ctgtcaccgg ggggatttcg
    25861 tccggtctat ttttatcagc acacatcagc ggctaagaaa taaccttcgc catgttgcgt
    25921 caccagtaaa tccgcgctga gtttatggcg taaacgacga attaacacat cgacggtgcg
    25981 caagtcaggg ttttccaccc gacgcgcaga gagcatacgc agcagacgtt cacggctaag
    26041 aatttcgccc ggattcgtca caaatgccac caacatttca tactctgcgc gggtcagttt
    26101 aatcggctcg ccatcccgct ccagcgtatg gcgcgacaca ttcaggcaat aaccggcaaa
    26161 gcgatagcag ttgtcctgtg tgtgcggttg agcttgtcgc gcgaggtcga ttcgccagag
    26221 cagatttttc acccgtacta ccagttcgcg cagttccagc ggtttggtga cgtaatcgtc
    26281 tgcgcccatt tccagcccaa caatacggtc aatccgatcg ctgcgtcccg taaccagaat
    26341 aatccccacc gttgagcgtt ctcgcagggc gcgggttaac atcaggccat tttcatcggg
    26401 caaattaata tccagcagaa ttaaatccac cggctgattc tgcataattt cccgcagccc
    26461 ggcaccgctc gccgtaacgg aaacggtata cccctcctga gtgaagtagg attgtaatcg
    26521 cgcctgggta accggctcat cttcaacaat aacaatgtga tgtggcatca gagggtttta
    26581 ctcattctgt tcatatctgt tcatattctg ccgtaagccg ttcatcctga ccagtgccgc
    26641 tgttcatatt tactcattaa gatcgcttca ctaaaccata attttacagg ggctattatg
    26701 cggaaactct ggaacgcgct acgccgaccc agtgctcgtt ggtcggtact ggcgctggtc
    26761 gctattggga ttgtgattgg cattgcgctg attgtattgc cacacgttgg gatcaaagtc
    26821 accagcacaa ccgaattttg tgtcagttgc cacagtatgc aaccggtgta tgaagaatat
    26881 aaacagtcgg tgcacttcca gaacgcctcc ggcgtgcgag ctgaatgcca tgactgtcat
    26941 atcccgccgg atattccagg catggtgaag cgcaaactgg aagcgagcaa cgacatttac
    27001 cagaccttta ttgcccactc cattgataca cctgaaaaat tcgaagccaa acgcgcggaa
    27061 cttgccgagc gtgaatgggc gcgaatgaaa gaaaacaact cggcaacctg tcgctcctgc
    27121 cataactacg atgcgatgga tcatgccaag cagcatcctg aagcggcgcg tcagatgaag
    27181 gtggcagcga aagataatca atcctgcatc gactgtcata aaggtattgc ccaccagtta
    27241 ccggatatga gtagtggctt ccgtaagcag ttcgatgagc tgcgcgccag tgctaatgat
    27301 agtggtgaca cgctgtactc cattgatatt aagccgattt atgcggcgaa aggcgataaa
    27361 gaagcgtctg gttctctgct gcctgcttcg gaagtgaaag tccttaaacg tgacggcgac
    27421 tggctgcaaa ttgaaatcac tggttggacg gaaagtgcag gacgtcagcg tgtactcacc
    27481 caattcccag gtaaacgcat ctttgttgcc tcgattcgtg gtgatgtgca gcagcaggtg
    27541 aaaacgctgg agaaaaccac cgttgccgac accaataccg agtggagcaa gttgcaggct
    27601 actgcgtgga tgaagaaagg cgacatggtg aacgatatca aaccgatctg ggcttatgcg
    27661 gattcgctgt acaacggcac ctgtaaccag tgccacggcg caccggaaat ctctcacttt
    27721 gacgctaacg gttggatcgg cacgctcaac ggcatgattg gctttaccag cctcgataaa
    27781 cgtgaagaac gcaccttgtt gaaatatctg caaatgaatg cgtctgacac cgcaggtaag
    27841 gctcacggcg ataagaagga agaaaaataa tgaacaataa cgatctcttt caggcatcac
    27901 gtcggcgttt tctggcacaa ctcggcggct taaccgtcgc cgggatgctg gggccgtcat
    27961 tgttaacgcc gcgccgtgca actgcggcgc aagcggcgac tgaggctgtc atctcgaaag
    28021 agggcattct taccgggtcg cactgggggg ctatccgcgc gacggtgaag gagggtcgct
    28081 tcgtggcagc aaaaccgttc gaactggata aatatccgtc gaaaatgatc gccggattgc
    28141 cggatcatgt acacaacgcg gcgcgtattc gttatccgat ggtacgcgtg gactggctac
    28201 gtaagcgcca tctgagcgac acctcccagc gcggtgataa ccgttttgtg cgcgtgagct
    28261 gggatgaagc cctcgacatg ttctatgaag aactggaacg cgtacagaaa actcacgggc
    28321 cgagtgcctt gctgaccgcc agtggttggc aatcgacggg gatgttccat aacgcttcgg
    28381 ggatgctggc gaaagcgatt gccttgcatg gtaatagcgt gggtacaggc ggagattact
    28441 ctaccggggc tgcgcaggtg atcctgccgc gcgtagtcgg ttcgatggaa gtgtatgaac
    28501 agcaaacctc ctggccgctg gtattgcaga acagcaaaac cattgtgctg tggggctccg
    28561 atttgctgaa aaaccagcaa gcgaactggt ggtgcccgga tcacgatgtt tatgaatatt
    28621 acgagcagct aaaagcgaaa gtcgccgccg gtgaaattga ggtcatcagc atcgatccgg
    28681 ttgtcacatc cacccatgag tatctggggc gcgagcatgt gaagcacatt gcggttaacc
    28741 cgcaaactga cgtgccgctg caactggctc tggcgcatac gctgtacagt gaaaacctgt
    28801 acgacaaaaa cttcctcgct aactactgtg taggttttga gcagttcctg ccgtatctgc
    28861 tgggtgagaa agacggtcag ccgaaagatg ccgcatgggc tgaaaaactg acaggtatta
    28921 atgccgaaac cattcgtggg ctggcgcggc agatggcggc gaacagaacg cagattattg
    28981 ctggctggtg cgtgcagcgt atgcagcacg gtgaacagtg ggcgtggatg attgtcgttc
    29041 tggcggcgat gttggggcaa attggcctgc caggtggagg ttttggtttt ggctggcact
    29101 ataacggcgc aggcacgccg gggcgtaaag gcgttattct gagtggtttc tccggctcta
    29161 cgtcgattcc gcctgttcac gacaacagcg actacaaagg ctacagcagc accattccga
    29221 ttgcccgttt tatcgatgcg atcctcgaac cggggaaagt gatcaactgg aacggtaaat
    29281 cggtaaaact accgccgctg aaaatgtgta tttttgccgg aactaacccg ttccatcgcc
    29341 atcagcagat caaccgcatt attgaaggct ggcgcaagct ggaaacggtt atcgccatag
    29401 ataaccagtg gacctcaacc tgccgctttg ccgatatcgt gctgcctgcg accacgcagt
    29461 ttgagcgtaa cgatctcgac cagtacggca accactccaa tcgtggcatt atcgccatga
    29521 aacaggtcgt gccgccgcag ttcgaggcgc gcaacgactt cgatattttc cgcgagctgt
    29581 gccgtcgctt taatcgcgaa gaagccttta ccgaagggct ggacgaaatg ggctggctga
    29641 aacgcatctg gcaggaaggt gtacaacagg gcaaaggacg cggcgttcat ctgccagcgt
    29701 ttgatgactt ctggaataat aaagagtacg tcgagtttga ccatccgcag atgtttgttc
    29761 gccaccaggc attccgcgaa gatccggatc tcgaaccgct gggcacgccg agtggcctga
    29821 ttgagatcta ctcgaaaacc atcgctgata tgaactacga cgattgtcag gggcatccga
    29881 tgtggtttga gaaaatcgaa cgctcccacg gtgggcccgg ctcgcagaag tatccgttgc
    29941 atctgcaatc tgtgcatccg gatttccgac ttcactcgca gttatgtgag tcggaaacgc
    30001 tgcgtcagca atatacggta gcgggcaaag agccagtgtt cattaacccg caggatgcca
    30061 gcgcgcgcgg tattcgtaac ggtgatgtgg tacgcgtgtt taacgctcgc ggtcaggtgt
    30121 tggcaggggc agtggtttct gaccgctatg cacccggcgt ggcgcgaatt catgaagggg
    30181 catggtacga tccagataaa ggcggcgagc ctggtgcgct gtgcaaatac ggtaacccca
    30241 acgtgttgac catcgacatc ggtacttcgc agttggcgca ggcgaccagt gcgcatacca
    30301 cgctggtgga aattgagaag tacaacggaa cagtggagca ggtgacggcg tttaacggcc
    30361 ctgttgagat ggtggcgcag tgcgaatatg ttcccgcgtc gcaggtgaaa tcatgaccac
    30421 gctgacagca caacagattg cctgtgttta cgcctggctg gcgcagttgt tctcccgtga
    30481 actggacgat gaacaactga cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt
    30541 gctgaaaagc gaaccgccgc tcactgcggc ggtgaatgag ctggaaaacc gtattgccac
    30601 tcagacagta cgtgacgatg ctcgtctgga actggccgct gacttttgcg gcctgttttt
    30661 gatgaccgac aaacaagctg cgctgccgta tgcatcggcc tacaaacagg acgagcaaga
    30721 gattaaacgc ttgttagttg aggcagggat ggaaaccagc ggcaatttca acgaaccggc
    30781 agaccatctg gcgatctatc tcgagttgtt gagtcatctg catttttcgc tgggagaggg
    30841 gaccgttcct gcgcgaagaa tcgacaatct gcggcaaaaa acactgacgg cgctgcgaca
    30901 atggttacca gagtttgctg cgcgttgcca tcagtacgac agtttcggtt tttacgcggc
    30961 actaagccag ttattgctgg tgttagtgga atgcgaccac caaaacagat aacgtcgttt
    31021 gttggcctga taagacgcgt ttagcgtcgc atcaggcatt agtgcgcagt tgccggatgc
    31081 ggcgagaacg ccttatccgg cctacgggaa cagtgctttt tgtgggcctg ataagatgcg
    31141 ttaagcgtcg catcaggcat tatggcgcag ttgccggatg cggcgagaac gccttatccg
    31201 gcctacggga acagtgcttt ttgtgggcct gataagacgc gttaagcgtc gcatcaggca
    31261 acaaactcac gggtgagcca caaaccggga aagccgctgg cgcagcaggc ggttttcctg
    31321 cttcaggtgc gcaatatcat ccattaacgt cagcgccacc gcgatccccg gccagtccag
    31381 agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca atggcggcat ggtcgtcaaa
    31441 tacccaggtt gtctcctgga tctcccgcgg ttcaaccacc cccaaaccga caatttcatt
    31501 caactcctct tcagagatgc cggtatgcag gcaaaattcg gtaatagtaa aagtcaccgt
    31561 aacattagcc attatgcttt cccccaatct ttacgtggat caaaagacga ctgggcgtct
    31621 gccagttgct gccacagcgc ggcagtgttt tcatccggtt tcggcggcat cacgattttc
    31681 agtaccgcat acagatcgcc ggtctgtttt ttgctcacca gacctttgcc tttaacgcgc
    31741 aatcgttgcc cggcctggct gccaggcggg atagtcagca aaatgctttc tttcagtgtt
    31801 ggaacggtga ctttagtacc cagcgccgct tcccacgggc taaccggcac cactatttcc
    31861 agatcctggc cgacaatatc aaacagcgga tgtggcgcaa tgtgaatcac cagccacagg
    31921 tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc ctttcaggcg gatgcgttga
    31981 ccattgccga cgcccgccgg gatcttcaca ttcagcgttt tcgggatttc ctgttcgatc
    32041 atgccaaagg cgttataaac cggcaggtta tagctgatgg tacgcttatg ctcagtaagc
    32101 gtttcttcga ggaaaaccgc cacttcgatt tcaatatcgt ggccgcgtgt ggcggggcgt
    32161 tgacggctct ggcgggcatg ctgaccgaaa attgacgaga agatatcgtc aaaatcttcg
    32221 gcgttaaaac tctgaccgtc gccatgatgg aactgacggt taaattgcgg atcgttgcga
    32281 tgttgccaca tctgatcata ctcagcgcga cgttgttcat cacttaacac ttcccaggct
    32341 tcggcgacct ctttgaagcg ggcttcggca tccggttctt tgctgacatc aggatggtat
    32401 ttgcgggcca gtcgacgata ggcggtcttg attgtcttga gatcgtccgt cggtttcacg
    32461 cccatgatgg cgtaataatc ctttaattcc atagcgttat ctcgcgtaaa tcaacacaaa
    32521 ttgaaggaac ccctgtaagg taactcctat aagtgtaggg taatcctcaa aatttcatat
    32581 gccaacacag aatatgttat tgaaatcatc gcggagggga ggtcgcctca aggtgggagg
    32641 aacatatttt atttacctcc ctcctgaact tttggctcat gcttacaagt taaacaataa
    32701 ggaaaggaat gcacaactcg actatttaaa tcgttatgca atttaatgat aaagacatgt
    32761 gctaaaaacc atttctatta gtctcacatt ctttttttat tggtaatggt aacgccgctg
    32821 attttcttta tagctactga tgtaatcatt agcggcgtaa acgaggccgg ggctaatcag
    32881 gcatagtttg cgtcaaaact tgcctgtttt taaaggagaa tatagaaaaa caggcgttca
    32941 acgagccatt ttgcgaacct gttcccggaa aaatgacata tttctgtcac actctttaat
    33001 tattgataac aaaagaggtg tgaggaatga acaaaacgct aatcgccgca actgtggcag
    33061 ggatagtttt actcgcttca aatgctcagg cacaaaccgt accggaaggc tatcagctac
    33121 agcaagtcct tatgatgagc cgtcataact tacgtgcgcc gctggcgaac aatggcagtg
    33181 tgctggagca gtcgacgccg aataaatggc ctgaatggga cgtccccggt gggcagctca
    33241 ccactaaagg gggcgtgctc gaagtgtata tgggccatta tatgcgtgaa tggctggcag
    33301 aacaggggat ggtgaaatca ggggaatgcc cgccaccgga caccgtttat gcctatgcca
    33361 atagtctgca acgcaccgtc gcgaccgcgc agttctttat tactggcgca ttccctgggt
    33421 gtgatattcc tgttcatcac caggaaaaaa tgggcactat ggaccccacc tttaacccag
    33481 tgatcaccga tgattccgcc gcattcagtg aacaggcggt ggcggcaatg gagaaagagc
    33541 tcagcaaact ccagcttacc gacagctaca agctactgga acaaatcgtt aactataaag
    33601 attctcctgc gtgtaaagag aagcaacaat gttcgcttgt agatggcaaa aataccttta
    33661 gcgcgaagta tcaacaagaa ccgggcgttt ccgggccgct gaaagtcggc aactcgctgg
    33721 tagatgcgtt tactttgcaa tactacgaag gttttccgat ggatcaggtg gcctggggag
    33781 aaatcaaatc tgaccagcag tggaaggtgt tgtcgaagct gaaaaacggt taccaggaca
    33841 gcctgtttac ctcaccggaa gtggcgcgca atgttgcgaa accgctggtc agttatatcg
    33901 acaaagccct ggtcaccgat cgcaccagtg caccgaaaat cacggtatta gttgggcacg
    33961 actccaacat tgcctctctg ttaacggcgc tggatttcaa accgtatcag ttgcatgacc
    34021 agaacgaacg cacgccgatt ggcggcaaaa tcgttttcca gcgttggcat gacagcaaag
    34081 ccaatcgcga tttgatgaaa attgaatatg tgtaccagag tgcggaacag ttacgtaatg
    34141 ccgatgcgtt aaccctgcag gcacctgcgc agcgcgtgac gctggaatta agcggttgcc
    34201 cgatagatgc caacggtttt tgcccaatgg ataagtttga tcgcgtattg aatgaagcgg
    34261 tgaaataaca gaaaactccc ccgcgtgaag cgggggagtc gctggttaaa cgtttttacg
    34321 ttcgatggtc tgttcgcccc aaaaaagcga atctttatcg gtcttagcaa aggctttgac
    34381 taacacttca tcgctacctt cttcccaaat cttttccgcc attttttcgt cgtacccggc
    34441 gacttcgaaa atggcctcgg ctatttccgg cgacgtattg cgcagagatg cccattcacc
    34501 gacgtgatga gctttcgctt cttgagttgg catgcgtatc ctcctgttga agattagctg
    34561 ttaagtttaa ctgccagacc tgcgacatat tccccttgat aacgagcaat agacagttct
    34621 tcctggctgg gctggcgtga accgtcaccg cctgcgatgg tggttgcgcc gtacggcgta
    34681 ccgccgcgaa cctgtgaaac gtcaaataat tcctgcgctg cgtagccaat ggggacaatc
    34741 accatgccgt gatgcgcaag ggtcgtccag gtggatgtga tggtttgttc ctgaccgccg
    34801 ccagtaccgg tggaactaaa gacgctcgcc agttttccgt atagtgcgcc ggaagcccac
    34861 aggccgcccg tctggtcgag gaaggtacgc atttgaccgg acatgttacc aaagcgggta
    34921 ggtgtaccaa aaataatggc gtcgtaatcg gccagttctt gcggggttgc aaccggtgca
    34981 gtttgcgttt taccgcctgc tttttcaaat aattgcggcg gcatggtttc cggtacacgc
    35041 ttaacgacaa cttctgcgcc atccactttg cttgcaccct cagcaactgc gcgtgccatc
    35101 gtttcaacat gtctaaggat atggtttatc attttgaaaa atataatttt atttcatcct
    35161 cctgatcact ttggggcacg tctggggcgc gggaattaag gatattattc aacatagcta
    35221 cctgagtcac gctgcactca ggcatccatg caccataaac attgtagacc atgctggcgc
    35281 tggagtgccc catctgtgac gcaataaatg tcgggtttgc tccggcagat aaagcccagc
    35341 acgcataggt atggcgtgac tgatacgctt tacgggatcg gatacccgct ctttttattg
    35401 ctgaatccca tgtcgctccg atggagctta ccgcgtagtt aatacccgcc ttgtgattct
    35461 tgcgaacgat ttgcggacag aaaacaaaag tgcactcgtg caaaattgtt cttccgtact
    35521 cgcgtaattt aacagtgatc tgatgctgcc tgccaagacg agtaagcatc gcctggtttt
    35581 taagtgcttc aattgctggt gccagaagat gtataacccg gttagtgcct gcgtcagtct
    35641 ttggtagcgt aaaatcacct atttttgtaa aatttcttcg cactgttatc gtgccagctt
    35701 tcaggtcgat atcctcccat gcaagtgcgg caatttcacc gtgtcgcatc cctgtaaaaa
    35761 cagccactgt ccagaggttt ttggtctgtt gatgatggca ggcatcaatg agacggctaa
    35821 actcatctct ggtcagtgga tccggcactg gttttgattt cctcagcggt gttattgaat
    35881 taaacgggtt tttctccaga tacccgtttt cggcggcaaa gctgaacatt ccggctgttg
    35941 ttgtcatgta atagttcact gtgggtacgg ttcttccttt ttgggacgtg ctggttttcc
    36001 tgcttccctt ttccccggtc agtaaatctt tcctgataaa cagcagatct tcttttgtaa
    36061 tcgatgacgc cagcctgcca ggaccaagtc tcggtagcat atttttcatc actgattgat
    36121 aacgatttaa ggcgttacta ctgatttcca ttgctttcag agtaagccat ttctgtgcca
    36181 gttcaccgac ggtgatatct ttttttacca ggccaaatag cttcaggtta ggtgagtcag
    36241 ggaatcgctc ggcataatca aacgttcccg ttctgattgc aaagcagaca gaagccctta
    36301 actcaccagc gatctttctg tttttcggag tatcgggcac gcgaagattt tcgcgcactc
    36361 gtttacccct gtacttaaac gttattcgga gttttcctcc gtgattttca acgccggctg
    36421 gataggctga attcgccatt gttcctcctg cgtccaagag cacgctcagg ttatactctt
    36481 atggacaaga attaaacatc ctgagatggt aagggttggt ttttgatcca agtgttgatg
    36541 gtaggaagat gatagagaca ttcgctgttt ttctttggcg ttccgtcggg ggcaatttgt
    36601 ttatattctc tgccgttcat ccacgcactg tccctggcgc gtaatattgt tccttttctt
    36661 aggcctgtta cagccattaa aagctccagt gttacccatt cattcgcatt tatctgtaca
    36721 acaggtgtga tgactggctg aatgccatgg ttatgttggt ttactaactc gcgcataagt
    36781 tgcctctgca tgtgtaagaa aaaaccgccc gaaggcggtt gtcagttgat tgatgtacgg
    36841 cgcatttttc gaaggctggc aatatgcttt tccttctcaa tttccgcttt aatcatatgc
    36901 agttcgttgt gctcgattcg ctcaaattct tcattaaatg cactaattga agtggctctg
    36961 gttctgccgt cgagccttcg gtagatcacc tgagtcagag ttaccttgca tatttctacc
    37021 ggatagttgt tggcatcaac gaaagactgc ccgcgctgga tcaggacgaa cacggtttgc
    37081 actccctgga tttccgctac gttcactctg ctaattcgtt gatcaggtca cgatatttat
    37141 taagttctcg caacgcttta catgcctctt cccaacgttt ctgtttccgt ccggcgcggc
    37201 gtgcttcttt ttgtgatttg cgcagcaggt cacctacaat gttg
//
LOCUS       DAZGHF010000032        36597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGHF010000032 DAZGHF010000000
VERSION     DAZGHF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.032"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..628)
                     /gene="yjgL"
                     /locus_tag="ACHK3A_001743"
     CDS             complement(<1..628)
                     /gene="yjgL"
                     /locus_tag="ACHK3A_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO8739831.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNDFARIAGGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVEIEITLSTITTMGTRQGHTAIIFQQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKEIARLLNNHQKLNNLQKLNNIQELNNSQE"
     gene            complement(751..1203)
                     /gene="tabA"
                     /locus_tag="ACHK3A_001744"
     CDS             complement(751..1203)
                     /gene="tabA"
                     /locus_tag="ACHK3A_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO8739832.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKANVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(1348..1941)
                     /gene="bdcR"
                     /locus_tag="ACHK3A_001745"
     CDS             complement(1348..1941)
                     /gene="bdcR"
                     /locus_tag="ACHK3A_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO8739833.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILEE"
     gene            2012..2725
                     /gene="bdcA"
                     /locus_tag="ACHK3A_001746"
     CDS             2012..2725
                     /gene="bdcA"
                     /locus_tag="ACHK3A_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8739834.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2857..3252
                     /gene="yjgH"
                     /locus_tag="ACHK3A_001747"
     CDS             2857..3252
                     /gene="yjgH"
                     /locus_tag="ACHK3A_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO8739835.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            3533..3667
                     /gene="pyrL"
                     /locus_tag="ACHK3A_001748"
     CDS             3533..3667
                     /gene="pyrL"
                     /locus_tag="ACHK3A_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO8739836.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3671..4606
                     /gene="pyrB"
                     /locus_tag="ACHK3A_001749"
     CDS             3671..4606
                     /gene="pyrB"
                     /locus_tag="ACHK3A_001749"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO8739837.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4619..5080
                     /gene="pyrI"
                     /locus_tag="ACHK3A_001750"
     CDS             4619..5080
                     /gene="pyrI"
                     /locus_tag="ACHK3A_001750"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO8739838.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5153..5539
                     /gene="ridA"
                     /locus_tag="ACHK3A_001751"
     CDS             5153..5539
                     /gene="ridA"
                     /locus_tag="ACHK3A_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO8739839.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5745..8441)
                     /gene="mgtA"
                     /locus_tag="ACHK3A_001752"
     CDS             complement(5745..8441)
                     /gene="mgtA"
                     /locus_tag="ACHK3A_001752"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO8739840.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGVYSRRYGWQ"
     gene            complement(8582..8635)
                     /gene="mgtL"
                     /locus_tag="ACHK3A_001753"
     CDS             complement(8582..8635)
                     /gene="mgtL"
                     /locus_tag="ACHK3A_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO8739841.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8820..9767
                     /gene="treR"
                     /locus_tag="ACHK3A_001754"
     CDS             8820..9767
                     /gene="treR"
                     /locus_tag="ACHK3A_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO8739842.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAYWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            9886..11307
                     /gene="treB"
                     /locus_tag="ACHK3A_001755"
     CDS             9886..11307
                     /gene="treB"
                     /locus_tag="ACHK3A_001755"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO8739843.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11357..13012
                     /gene="treC"
                     /locus_tag="ACHK3A_001756"
     CDS             11357..13012
                     /gene="treC"
                     /locus_tag="ACHK3A_001756"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO8739844.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKASPYRQFYIWRDGESETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRRLMTVGEMSSTSLEHCQRYATLTGSELSMTFN
                     FHHLKVDYPSGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13397..15535
                     /gene="nrdD"
                     /locus_tag="ACHK3A_001757"
     CDS             13397..15535
                     /gene="nrdD"
                     /locus_tag="ACHK3A_001757"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO8739845.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRSQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15693..16157
                     /gene="nrdG"
                     /locus_tag="ACHK3A_001758"
     CDS             15693..16157
                     /gene="nrdG"
                     /locus_tag="ACHK3A_001758"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO8739846.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16202..16588)
                     /gene="cybC"
                     /locus_tag="ACHK3A_001759"
     CDS             complement(16202..16588)
                     /gene="cybC"
                     /locus_tag="ACHK3A_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO8739847.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16771..18123)
                     /gene="pmbA"
                     /locus_tag="ACHK3A_001760"
     CDS             complement(16771..18123)
                     /gene="pmbA"
                     /locus_tag="ACHK3A_001760"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO8739848.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18217..18768
                     /gene="yjgA"
                     /locus_tag="ACHK3A_001761"
     CDS             18217..18768
                     /gene="yjgA"
                     /locus_tag="ACHK3A_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO8739849.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIEQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18926..20299)
                     /gene="mpl"
                     /locus_tag="ACHK3A_001762"
     CDS             complement(18926..20299)
                     /gene="mpl"
                     /locus_tag="ACHK3A_001762"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO8739850.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20475..21473
                     /gene="fbp"
                     /locus_tag="ACHK3A_001763"
     CDS             20475..21473
                     /gene="fbp"
                     /locus_tag="ACHK3A_001763"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO8739851.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21506..22501)
                     /gene="yjfF"
                     /locus_tag="ACHK3A_001764"
     CDS             complement(21506..22501)
                     /gene="yjfF"
                     /locus_tag="ACHK3A_001764"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO8739852.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22488..23510)
                     /gene="ytfT"
                     /locus_tag="ACHK3A_001765"
     CDS             complement(22488..23510)
                     /gene="ytfT"
                     /locus_tag="ACHK3A_001765"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO8739853.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            complement(23524..25026)
                     /gene="ytfR"
                     /locus_tag="ACHK3A_001766"
     CDS             complement(23524..25026)
                     /gene="ytfR"
                     /locus_tag="ACHK3A_001766"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO8739854.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25166..26122)
                     /gene="ytfQ"
                     /locus_tag="ACHK3A_001767"
     CDS             complement(25166..26122)
                     /gene="ytfQ"
                     /locus_tag="ACHK3A_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO8739855.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26432..26962
                     /gene="ppa"
                     /locus_tag="ACHK3A_001768"
     CDS             26432..26962
                     /gene="ppa"
                     /locus_tag="ACHK3A_001768"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO8739856.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27335..27841)
                     /gene="mug"
                     /locus_tag="ACHK3A_001769"
     CDS             complement(27335..27841)
                     /gene="mug"
                     /locus_tag="ACHK3A_001769"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO8739857.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            27966..28041
                     /locus_tag="ACHK3A_001770"
     tRNA            27966..28041
                     /locus_tag="ACHK3A_001770"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27999..28001,aa:Ile,seq:cat)
     gene            complement(28141..28986)
                     /locus_tag="ACHK3A_001771"
     CDS             complement(28141..28986)
                     /locus_tag="ACHK3A_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8739858.1"
                     /translation="MSDITISRPELVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKNEVFERGVINVFRGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLNSGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     QLSAI"
     gene            complement(29071..29268)
                     /locus_tag="ACHK3A_001772"
     CDS             complement(29071..29268)
                     /locus_tag="ACHK3A_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO8739859.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(29280..29768)
                     /locus_tag="ACHK3A_001773"
     CDS             complement(29280..29768)
                     /locus_tag="ACHK3A_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO8739860.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLTELINNVAENGYS
                     LRVVEESDQQSTCTPPPFATLAGIRCSTAHITETDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAGCLPGLPTF
                     DW"
     gene            complement(29765..30142)
                     /gene="cbtA"
                     /locus_tag="ACHK3A_001774"
     CDS             complement(29765..30142)
                     /gene="cbtA"
                     /locus_tag="ACHK3A_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFO8739861.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRVTG
                     LMTRDNYRTVNNITLGKYQEAKR"
     gene            complement(30231..30599)
                     /gene="cbeA"
                     /locus_tag="ACHK3A_001775"
     CDS             complement(30231..30599)
                     /gene="cbeA"
                     /locus_tag="ACHK3A_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO8739862.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            complement(30675..30896)
                     /locus_tag="ACHK3A_001776"
     CDS             complement(30675..30896)
                     /locus_tag="ACHK3A_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO8739863.1"
                     /translation="MKIVTRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            complement(30965..31441)
                     /gene="radC"
                     /locus_tag="ACHK3A_001777"
     CDS             complement(30965..31441)
                     /gene="radC"
                     /locus_tag="ACHK3A_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO8739864.1"
                     /translation="MQQLSFLPGAMTPGELSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(31456..31929)
                     /locus_tag="ACHK3A_001778"
     CDS             complement(31456..31929)
                     /locus_tag="ACHK3A_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8739865.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(32063..32263)
                     /locus_tag="ACHK3A_001779"
     CDS             complement(32063..32263)
                     /locus_tag="ACHK3A_001779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739866.1"
                     /translation="MKHIPEGVIQAPVSPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
     gene            complement(32263..33084)
                     /locus_tag="ACHK3A_001780"
     CDS             complement(32263..33084)
                     /locus_tag="ACHK3A_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8739867.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVQKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(33262..33393)
                     /locus_tag="ACHK3A_001781"
     CDS             complement(33262..33393)
                     /locus_tag="ACHK3A_001781"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO8739868.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            complement(33487..33948)
                     /locus_tag="ACHK3A_001782"
     CDS             complement(33487..33948)
                     /locus_tag="ACHK3A_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO8739869.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKMLTDP"
     gene            complement(34024..36540)
                     /locus_tag="ACHK3A_001783"
     CDS             complement(34024..36540)
                     /locus_tag="ACHK3A_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739870.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLNGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDATLQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLVLVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVAGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRQEEALLPELPA"
BASE COUNT         8898 a         9693 c         9252 g         8754 t
ORIGIN      
        1 actcctgcga attatttaac tcctgtatat tatttaactt ctgtaaatta tttaacttct
       61 gatgattatt taatagacgc gctatttcct tgttagcttc gcgttggtaa cacgccagaa
      121 ccttgttagt aatcaggtgt aatgaactat catcttttct ttcaaaagac ttcccttcat
      181 aacgattagt cgaaccatca gattgttgaa atataatagc cgtatgtcct tgacgagtcc
      241 ccatagtagt gatagtggaa agtgtaattt ctatttccac cttagataaa tccgctcgtt
      301 ccccttcagg attattcatg taaaacttca ttttttcatt gccatcagta tctttagaat
      361 aagatgagga ctcaatatga gccatcatat ttctacccgc ttcgttggag cgtttgctaa
      421 aatccacata acccagggta aatatctttg ccaaaaactc taaaaatgct ccaggagaaa
      481 gaatattcct tctgctatcc tgccccccgg caatacgggc aaaatcattc attccaacac
      541 gccaggtatt taaaacttca cttttttgtt taaaattgtc ggctaatgta atgtgttgag
      601 aataattcaa atctgatatt ttgctcatga cgaactcctt gagttaataa gaacgtctaa
      661 gcattacaaa ttttactcac aaaaaaatat caaaaaacgc tcttttcaca aactcgccgt
      721 aaacctggag ttatttacgg cgaaaagtca tcacgccatc agcatcttca ctactgcttt
      781 gcgaacccgg gctggcgcgc ccactgcgca cagcggttta tgcacttccc ccggataaaa
      841 cacaacaaaa tcaccttcat taaggataac tgttttctca tcaacgcctt ccggcaaaaa
      901 tgcgatgtct ttatcagcta accagtcggt atccggcgta cctgcaggtt gtgtgctgaa
      961 ggtcatgcct tcctgacctt ttaacacaat ctgaatgtcg agatagcggg catggtactc
     1021 cgcacggcga gcttcgtacg gctcggtcat atcttccgag ataagataaa acagacgatt
     1081 gccttcgata tcgtgcttgc ccttgggcgt ttctgccgta acgtttgctt tgatatgctc
     1141 aatcgcctgg cgtaactctt ggggtagcca cggctgaaga ttatgaatat ttccgatgat
     1201 catcttttcc actccttaat taaaacaacg tttcatttct atcgttatac gaaaagatga
     1261 tcaattccgc tagccttaac gcgtgaatta ttgtcttatc acaacttatc ggcaggcgcg
     1321 ttcccgcctt tcgggtcaag gcccgcatca ctcctcgaga atagttttta tcgcttcccc
     1381 cgccagggca gctgttgcac agagttgttc gatggagtgc ccctcccgtg ccttcgcaga
     1441 aagccctgac atcacggtac tcataaaatc agtcacacat tgtgcgcttt gtggatgccg
     1501 cctggcgata tagtcataaa tggtcgtttc tgcggcgtga taatattgaa tggcaatatc
     1561 acgcgcttgt ggatcatgac tatgaatacc ttcaagaacc atacagccag cgcagccgcc
     1621 gttttggcta tatctgcgcg ccgcttcttt taatacctca accaggcact cgcctaccgg
     1681 acgatcatca cgaagaatat cggcaagcgg aatagcttcc gtaccgacat attcattgag
     1741 tacacgacta aacaacccag ccttactgcc aaaagccgcg tagaggctcg gggggttaat
     1801 accaagataa tcagtaactt cagagacact tactgcatcg aaaccttttt ggtgaaaaag
     1861 cacttttgcc gcagagactg cctgctcagg agcgaaacgt ctggggcgac ctggaacgcg
     1921 agattgtttt ttagtgacca ttacaaaact tgttgacaga aagttaaaac agttttgtaa
     1981 tgcatgttac ataataaatc aaggagtcct tatgggcgct tttaccggta agacagttct
     2041 catcctcggt ggtagccgtg gaatcggtgc cgctatcgta cgtcgtttcg tcaccgatgg
     2101 agccaatgta cgattcacct atgcggggtc gaaagatgcc gctgaacgtc tggcacaaga
     2161 gactggagcg acagcagtat tcacagatag tgctgacaga gacgctgtta ttgatgtcgt
     2221 tcgtaagagc ggcgcattgg atatcctggt ggtaaatgca ggtattggcg tctttggcga
     2281 tgccctggaa ttaaatgccg acgatattga tcgccttttc aaaatcaata ttcatgctcc
     2341 ttatcatgcc tctgttgaag ccgcccggca gatgcccgaa ggcgggcgca tcttaatcat
     2401 cggctccgtg aatggcgatc gtatgcctgt tgcaggcatg gctgcttatg ccgccagtaa
     2461 atctgccctg caaggcatgg cgcgcgggct ggcccgtgat tttggaccgc gagggatcac
     2521 catcaacgtc gtccagccag gaccaattga taccgacgct aatcccgcca acgggccaat
     2581 gcgcgatatg ttgcatagtt ttatggccat caaaagacat gggcaaccgg aagaggtcgc
     2641 tggtatggtc gcatggttag cagggccaga agccagtttt gttaccggcg cgatgcatac
     2701 cattgatggc gcgtttggcg cataaccgac tacgctcaat taagcccagc cattatccat
     2761 gatgtctggg ttttgtttac taacgtcgtc cccctaaaag cggctcccgg taaatataaa
     2821 tcttctgttc atgtcgtaaa tcttgaggat aaccaaatgg tagaaagaac cgctgttttc
     2881 cctgctggcc gacattcact gtatgctgag catcgttatt ctgcggctat tcgttccggc
     2941 gatttgctgt ttgtttccgg gcaagtagga agtcgagagg acggaacacc agaacccgat
     3001 tttcagcaac aagtcagact ggcatttgat aatttgcatg cgaccctggc tgctgcggga
     3061 tgcacttttg acgatatcat tgatgttacg agctttcata ccgatccaga aaaacaattt
     3121 gaagacatca tgacggtcaa aaatgaaata tttagcgccc caccttatcc aaactggacg
     3181 gcggtgggtg ttacctggct ggcaggcttt gattttgaaa ttaaagtgat agcgcgcatc
     3241 cctgagctat aagcaatagt gttagccgtt cgttttcaca ctccgcccta taagtcggat
     3301 gaatggaata aaatgcatat ctgattgcgt gaaagtgaaa aagaaaaaag caggaaatgt
     3361 ctgcaattat tgataccgaa cgacagttct cccccagaat cacatcaaat aaaaatgcat
     3421 gtattttgac ttttaattca aataaaccgt ttgcgctgac aaaatattgc atcaaatgct
     3481 tgcaccgctt ctgacgatga gtataatgcc ggacaatttg ccgggaggat gtatggttca
     3541 gtgtgttcga cattttgtct taccgcgtct gaaaaaagac gctggcctgc cgtttttctt
     3601 cccgttgatc acccattccc agcccctcaa tcgaggggct tttttttgcc caggcgtcag
     3661 gagataaaag atggctaatc cgctatatca gaaacatatc atttccataa acgaccttag
     3721 tcgcgatgac cttaatctgg tgctggcgac agcggcgaaa ctgaaagcaa acccgcaacc
     3781 agagctgttg aagcacaaag tcattgccag ctgtttcttc gaagcctcta cccgtacccg
     3841 cctctctttc gaaacttcca tgcaccgcct gggtgccagc gtggtgggct tctccgacag
     3901 cgccaataca tcactgggta aaaaaggcga aacgctggcc gataccattt cggttatcag
     3961 cacttacgtc gatgcgatag tgatgcgtca tccgcaggaa ggtgcggcgc gcctggccac
     4021 cgaattttct ggcaatgtac cggtactgaa tgccggtgat ggctccaacc aacatccgac
     4081 gcaaaccttg ctggacttat tcaccattca ggaaacccag gggcgtctgg acaatctcca
     4141 cgtcgcaatg gttggtgacc tgaaatatgg ccgcaccgtt cactccctga cccaggcgct
     4201 agcgaagttc gacggcaacc gtttttactt tatcgcgccg gatgcgctgg caatgccgca
     4261 atacatcctt gatatgctcg atgaaaaagg gatcgcctgg agtctgcaca gctctattga
     4321 agaagtgatg gcggaagtag acatcctgta catgacccgc gtgcaaaaag agcgtctgga
     4381 cccgtccgag tacgccaacg tgaaagcgca gtttgttctt cgcgccagcg atctccacaa
     4441 cgccaaagcc aatatgaaag tgctgcatcc gctgccgcgt gttgatgaga ttgcgacgga
     4501 tgtggataaa acgcctcacg cctggtactt ccagcaagca ggcaacggga ttttcgctcg
     4561 ccaggcgtta ctggcactgg ttctgaatcg cgatctggta ctgtaagggg aaatagagat
     4621 gacacacgat aataaattgc aggttgaagc tattaaacgc ggcacggtaa ttgaccatat
     4681 ccccgcccag atcggtttta agctgttgag tctgttcaag ctgaccgaaa cggatcagcg
     4741 gatcaccatt ggtctgaacc tgccttctgg cgagatgggc cgcaaagatc tgatcaaaat
     4801 cgaaaatacc tttttgagtg aagatcaggt agatcaactg gcattgtatg caccgcaagc
     4861 cacggttaac cgtatcgaca actatgaagt ggttggcaaa tcgcgcccca gtctgccgga
     4921 gcgcatcgac aatgtgctgg tctgcccgaa cagcaactgt atcagccatg ccgaaccagt
     4981 ttcttccagc tttgccgtgc gaaaacgcgc caatgacatc gcgctcaaat gcaaatactg
     5041 tgaaaaagag ttttcccata atgtggtgct ggccaattaa ttgcggttgg taataaaagt
     5101 ctggctccct ataatgagcc agacttttta ccgctgtaat aaaggagaaa tcatgagcaa
     5161 aactatcgcg acggaaaatg caccggcagc tatcggtcct tacgtacagg gcgttgatct
     5221 gggcaatatg atcatcacct ccggtcagat cccggtaaat ccgaaaacgg gcgaagtacc
     5281 ggcagacgtc gctgcacagg cacgtcagtc gctggataac gtaaaagcga tcgtcgaagc
     5341 cgctggtctg aaagtgggcg acatcgttaa aaccaccgtg ttcgtgaaag atctgaacga
     5401 cttcgcaacc gtaaacgcca cttacgaagc gttcttcacc gagcacaatg ccaccttccc
     5461 ggcacgttct tgcgttgaag ttgcccgtct gccgaaagac gtgaagattg agatcgaagc
     5521 aatcgctgtt cgtcgctaat cttgatggaa atccgggcta tcatgcccgg attaagtctg
     5581 atgacaaaag caaaatcgcc cgatgcgcta cgcttatcag gcctacgtga ttcctgcaat
     5641 ttattgaatt tgtaggccgg attgggcgtt tacgccgcat ccggcatgaa caaagctcac
     5701 tttgtctgca atctgaatcc gggccaccat gcccagttta ttctttactg ccagccgtaa
     5761 cgacggctat agaccccttt caccaactgg gttaacgtca tataccctgc cagaatcgca
     5821 accagccacg ggaaatagct taacggcagc gcctgcagct gcagataact ggccagcggt
     5881 gaaaacggca atgcgatccc gacaatcatc acgatcacgg tcatgatcat taacggccac
     5941 gatgcacagc tctgaataaa cggtacacga cgggtacgga tcatatgcac aatcagcgtt
     6001 tgcgacagta agcccaccac aaaccagccc gactggaaca gcgtttgcgt ttccggcgtg
     6061 ttggcatgga atacccacca catcaggcaa aacgtcagaa tatcgaagat cgagctgatc
     6121 ggtccgaaga agatcataaa gcgccccaga tccgccggat tccaacgctg cggcttttga
     6181 atttgctcgt cgtcgacgtt atcaaacggg atcgccacct gtgacacatc gtacagcaag
     6241 ttctgaataa gcaagtgtaa cggcaacatc ggcaggaagg gcaagaaagc actcgccacc
     6301 agcacgctga acacattacc gaagttagaa ctcgccgtca ttttgatgta tttcagcatg
     6361 ttggcgaaag tgcgacgtcc ctcaataacc ccctcttcca gcaccatcag gcttttttcc
     6421 agcaggatga tatcagccgc ttcacgggca atatctaccg cgccgtccac agaaatgccg
     6481 atatccgcag cgcgtaaggc gggcgcatca ttaataccat cgcccataaa gccaaccaca
     6541 tgcccttcgc gcttcagtaa ggtcacaatg cgttctttat gcatcggcgt caggcgggca
     6601 aacagcgtgg tgcgctgtgc gagatttgcc agttcatcgt cagatagcgt ttcaatatca
     6661 ctaccaatga ccacctctcc cgcatccagc cccacttcat ggcacacttt cgctgcaact
     6721 aactcactgt cgccagtaag gatttttacg gtaattccac tcgcttttaa tgccttcagt
     6781 gccggagcgg ttgtctcttt tggcggatca aggaaagcaa tatatccttc gaggatcagg
     6841 tcggattcat ccgcccgctg gtaatctcct tcacgcgctg gcaggtattt cgtcgccacc
     6901 gcaaccacgc gcagcccctg acgattcagc gtatcagtaa cccgcttaat cttacgcagc
     6961 atgatgtcat cgagcggcac aatctcgcca ttgtgacgca cctgcgaaca cacattgagg
     7021 atttcctgca atgcaccttt gcaaaccagc tgatggtgtt cggtattttc tgccactacc
     7081 accgacatcc gacgacgctc gaaatcaaac ggaatctcat caattttttg ccaacggctg
     7141 gccagcgaac gcgctgactc ttcatccgta ccttcgagca ccgcggtatc aagcaggttt
     7201 ttaagtccgg tctggtaatg actgttcaac cacgcgctat gcagcacgcg ttcgctggtt
     7261 ttaccggaga tatcggtatg attctccagc acaattttgt cctgcgtcag ggtgccggtt
     7321 ttatcagtgc acagaatatc cattgcgcca aagttctgaa tggcatccag atgtttgacg
     7381 atcactttct gtttcgacag ctttactgcg ccgcgcgcca gcgtcgaggt aacaatcatc
     7441 ggcaacattt ctggcgttaa gcctaccgcg accgaaagcg caaacagtgc cgcttcccac
     7501 cagtcgcctt tggtgtaacc gttgataagc aacaccaccg gagccatcac cagcataaag
     7561 cgaatcagca gcatactgac gcggctaatc ccttgctgaa aggcattcgg ctcgctttcc
     7621 tgctcactaa cacgccccgc cagttgacca aaccaggtat tggcccctgt ggcaatcacc
     7681 attgcttgtg ccgtaccgct caccacggtg gtgcccataa aacacagggt gtcgcactcc
     7741 agcggattgc tgtgctccgg ctggcgagta gtagcggctt tttccacggg cagagactca
     7801 ccggttaacg acgcctgagc gacgaacaga tcccgcgcct gcaagatacg taaatctgcc
     7861 gggatcatat cccctgccgc cagttttata atatcgccgg gcaccagttg gtcgatcggg
     7921 atctccagcc agccattttc gcctttgtcg ttaattacgc gcagcaccgt cgcagtattg
     7981 ctgaccatcg ctttcagggc atctgccgct ttagtggaac gtgcttcctg aataaagttc
     8041 agcaacgtag aaatagcgac cattagcgcg ataacgcccg cggcaaataa atcttccgtg
     8101 gcgtaagaaa tagcgccgag aatggtgagt aaaatattaa aggggttgcg atagcagacc
     8161 cataaatgaa cccaccacgg cgacggttgt tgtgcgggta atttattttc accatgttgt
     8221 tcgcgggcag attccacttc cgcctgattt aatccttccg gatgggtgtc gaacgttttc
     8281 cacaattctt cttcgggcat caccgccatt ttcaggcaat gcgcacttaa ggacggcggg
     8341 accgccgtat tcactgtctg ctgcgcgcca ggcaatggat cgcgatgaac cagacgggaa
     8401 ggtaaatggc gaatgaaccg ggtaaaaatt tctttaaaca taaggagtcc ctccgcactg
     8461 tctgaaacag tacagaaatg tcttcaggcg tggcgaagcc ttacctgaag gtaataaagc
     8521 tgagcaggga cgttaaaacg tcacggcctt acgcatccgg taacgccgtg aaaagacggg
     8581 cttaccggaa aagttttaat ctccgtcgag ggagaggcgt gggatcaggt tccatataac
     8641 ctccggtaag tgaaaaaagc gccgcaaata ataaggagaa tccaataata ttcgtagcat
     8701 ttatggcgat ataatacctg ctggcaacta aaccaacgat aaaccagact ttacccattg
     8761 ctgaatgcac aggtaaagtt aggctcaaat aattaaacaa cacgttatgg gacaacagga
     8821 tgcaaaatcg gctgaccatc aaagacatcg cgcgcttaag cggcgtgggg aaatctacgg
     8881 tttcccgggt gctgaataac gaaagcggcg tgagccagcg cacgcgcgag cgtgttgaag
     8941 cagtgatgaa tcagcatgga ttttcccctt cccgctctgc gcgcgctatg cgtgggcaaa
     9001 gcgataaagt ggtcgccatc attgttaccc gtctggattc gttgtcagaa aatctcgccg
     9061 ttcaaaccat gctgccagcg ttctatgaac aaggttacga tccaatcatg atggaaagtc
     9121 agttttcccc acaattagtt gccgaacatt tgggggtgct gaaacggcgt aatatcgacg
     9181 gcgtagtgct gttcggtttt accggcataa cagaagaaat gttagcctac tggcagtcat
     9241 cgctggttct gctggcgcgt gacgcaaaag gctttgcttc ggtctgttat gacgacgaag
     9301 gggcaatcaa gatcctgatg caacggctgt atgaccaggg acatcgtaat atcagttatc
     9361 tcggcgtgcc gcacagtgac gtgacgaccg gtaagcgacg tcacgaagcc tacctggcgt
     9421 tctgcaaagc gcataaattg catcccgttg ccgctctgcc agggcttgct atgaagcaag
     9481 gctatgagaa cgtagcaaaa gtgattacgc ctgaaactac cgcgttactg tgcgcaaccg
     9541 acacgctggc acttggcgca agtaaatacc tgcaagagca acgcatcgac accttgcaac
     9601 tggcgagcgt cggtaatacg ccgttaatga aattcctcca tccggagatc gtcaccgtag
     9661 atcccggtta cgccgaagct ggacgccagg cggcctgcca gttgatcgcg caggtaaccg
     9721 ggcgcagcga accgcaacaa atcatcattc ccgccaccct gtcctgatcg tttcctgaac
     9781 gataaattgt gatcttcgct gcgtttcggg aacgttcccg tttttaaatt tttcagcgca
     9841 atatattctg cagccaacca aaaatgtcat ctgccatggg gctttatgat gagcaaaata
     9901 aaccaaacgg atatcgatcg gttgattgaa ctggtcggcg ggcgcggcaa tattgcgacg
     9961 gtgagccact gtattactcg tctgcgcttt gtcctcaacc aaccggccaa tgccagaccg
    10021 aaagaaattg agcaactccc catggtgaaa ggctgtttca ccaatgccgg gcaatttcag
    10081 gtggtgattg gcaccaacgt gggtgattac tatcaagcac tgatagcgtc aaccggacag
    10141 gcgcaggttg ataaagagca ggtaaaaaaa gccgcccggc agaatatgaa atggcatgag
    10201 cagttgatct ctcatttcgc ggagattttc ttcccgttgc tgcccgcgtt gattagcggc
    10261 ggtttgatcc tcggttttcg caatgtgatc ggcgatttgc ccatgagcaa cggtcaaacg
    10321 ctggcgcaaa tgtacccttc cctgcaaacg atctacgatt ttctgtggtt gatcggtgaa
    10381 gcgatcttct tctacctgcc ggtcgggatt tgctggtcag cggtgaaaaa aatgggcggc
    10441 acgccgatcc ttggtatcgt gcttggcgta acgctggttt ctccacagct gatgaacgct
    10501 tatctgctcg ggcagcagct gccggaagtg tgggactttg gcatgttcag catcgccaaa
    10561 gtgggctatc aggcgcaggt gatcccggca ctgttagccg ggctggcgct gggcgttatt
    10621 gaaactcgtc ttaagcgcat cgtgccggat tacctctatc tggtggtggt gcccgtttgt
    10681 tcgctgatcc tcgcggtgtt cctcgcccat gcgctgattg gtccgtttgg tcgcatgatt
    10741 ggcgatggcg ttgcctttgc ggtacgtcac ctgatgaccg gcagctttgc tccgattggc
    10801 gcggcattgt ttggcttcct gtacgccccg ctggtgatca ccggcgtaca tcagaccacg
    10861 cttgctattg atttgcagat gattcaaagc atgggtggta cgccagtgtg gccgctgatt
    10921 gcgctgtcga atatcgctca gggttccgcc gtgataggca ttatcatttc cagccgcaag
    10981 cacaatgaac gcgagatctc cgtgcctgcc gctatctccg cctggcttgg agtcactgag
    11041 cctgctatgt acggcatcaa cctgaaatat cgcttcccga tgctgtgcgc gatgattggt
    11101 tctggtctgg caggattgct atgcggcctg aacggcgtta tggcgaatgg catcggcgta
    11161 ggcggcctgc cgggaattct ctcgattcaa ccgagctact ggcaggtgtt tgcgctggca
    11221 atggctatcg ccatcatcat cccgattgta ctcacctcgt ttatctatca gcggaaatac
    11281 cgcctgggca cgctggacat tgtttaattt tcttcggggc gcaattgcgc cccctcgcat
    11341 ttgcaggaat aacgtaatga ctaatcttcc ccactggtgg caaaacggcg ttatctacca
    11401 gatttatcca aagagttttc aggacaccac gggtagcggc accggcgatt tacgtggcgt
    11461 tatccagcgc ctggactatc tgcataaact gggcgttgat gccatctggc taaccccctt
    11521 ttatgtctct ccccaggttg ataacggtta cgacgtagcg aactatacgg ctattgatcc
    11581 cacctacggc acgctggacg attttgacga actggtgaca caggcaaaat cgcgcgggat
    11641 tcgtatcatt ctcgatatgg tgtttaacca tacctctacc caacatgcct ggtttcgcga
    11701 ggcgctgaac aaagcaagcc cttaccgcca gttttatatc tggcgcgatg gagaatcaga
    11761 aacgccaccg aacaactggc gttcaaaatt tggcggtagt gcgtggcgct ggcatgcgga
    11821 aagcgaacag tactatttgc atctctttgc accagaacag gcggatctca actgggagaa
    11881 tccagcggta cgcgcagagc tgaaaaaagt ctgtgagttc tgggccgatc gtggggtcga
    11941 cgggttgcgc ctggatgtgg tgaatctgat ctccaaagac ccgcgtttcc ctgatgacct
    12001 ggatggcgac gggcgtcgct tctacaccga cgggccacga gcacacgagt ttttgcacga
    12061 gatgaaccgc gatgtgttta cgccacgcag gttaatgacc gtaggtgaaa tgtcctccac
    12121 cagccttgag cattgccagc gatatgcgac actgacaggc agtgaattgt cgatgacctt
    12181 taattttcat cacctgaaag tcgattatcc cagtggtgaa aaatggacgc tggctaaacc
    12241 tgactttgtg gcgttgaaaa cattgttccg ccactggcaa caaggaatgc acaacgtggc
    12301 atggaatgcc ttgttctggt gtaaccacga tcagccgcgc attgtttctc gctttggtga
    12361 tgaaggtgaa taccgcgtgc ctgcggcaaa aatgctggcg atggtgctgc atggcatgca
    12421 gggaacgccg tatatctacc agggcgaaga gattggcatg accaacccgc atttcacgcg
    12481 cattactgac tatcgcgacg tggagagcct caatatgttt gccgagctgc gcaacgatgg
    12541 tcgtgatgcc gacgagttat tggcaatcct cgccagtaaa tcccgtgaca acagtcgcac
    12601 acccatgcaa tggagcaacg gcgataatgc cgggtttacg gctggcgaac cgtggattgg
    12661 cctgggcgat aactatcagg aaatcaacgt agaagcagcg ctggccgatg agtcctcggt
    12721 gttttacacc taccaaaagt taatcgcact gcgtaagcaa gaagccgtcc tgacatgggg
    12781 cgattaccag gatctgctgc caaacagccc tgtattgtgg tgctatcgcc gtgagtggaa
    12841 ggggcaaacc ttactggtca ttgccaacct tagccgtgag acccaaccct ggcagccagg
    12901 gaaaatgctc ggcaactggc agcttgtgat gcataactac gaagaagcct caccacaacc
    12961 ctgtgccatg actttacggc cttttgaggc tgtctggtgg ttacagaagt aagtatccgc
    13021 taagccccgg taatactggt cattccgggg ttttgccttt cagcatgttg attttataat
    13081 caaaaaattc attaaaaaat ttacataacg ctgtagcgcc cgtcatccgt acgctctgct
    13141 ttttactttg agctacatca aaaaaagttc aaacatcctt gatgcaaagc actatatata
    13201 gactttaaaa tgcgtcccga cccaatatgt tgtattaatc gactataatt gctactacaa
    13261 ctccccacga aaaaggtgcg gcgttgtgga taagcggatg gcgattgcgg aaagcgccgg
    13321 aaaacgaaac gaaaaaaccg gaaaacacct ttcccaattt ctgtggataa cctgttctta
    13381 aaaatatgga gcgatcatga caccgcatgt gatgaaacga gacggctgca aagtgccgtt
    13441 taaatcagag cgcatcaaag aagcgattct gcgtgcagct aaagcagcgg aagtcgatga
    13501 tgcagattat tgcgccactg ttgccgcggt tgtcagcgag cagatgcagg gccgcagcca
    13561 ggtggatatc aacgagatcc agaccgccgt tgaaaaccag cttatgtcgg gtccatacaa
    13621 acaactggct cgtgcttaca tcgagtaccg tcacgatcgc gacattgaac gtgaaaaacg
    13681 cggtcgcctg aatcaggaga tccgtggtct ggttgagcag accaacgcct cgttgctcaa
    13741 cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag cgcgacctgc tggccgggat
    13801 tgtggctaaa cactatgcac gccagcacct gctgccgcgt gacgtggtgc aggcacatga
    13861 acgtggtgat attcactatc acgatctcga ttactcaccg ttcttcccga tgttcaactg
    13921 catgttgatc gacctgaaag gcatgctgac ccagggcttt aaaatgggga acgccgagat
    13981 tgaaccgccg aagtcgatct ctacggcaac cgcggtaact gcgcagatta ttgcccaggt
    14041 cgccagccat atttatggcg gcaccaccat taaccgtatc gatgaagtgc tggcaccgtt
    14101 tgtcaccgcc agctacaaca aacatcgcaa gaccgcagaa gagtggaaca tcccggacgc
    14161 cgaaggctac gctaactctc gtaccatcaa agagtgctac gatgccttcc agtcgctgga
    14221 gtacgaagta aacaccctgc acaccgccaa cggtcaaacg ccgtttgtaa cctttggttt
    14281 tggtctgggc accagctggg agtcgcgcct gattcaggaa tctatcctgc gtaaccgtat
    14341 cgccggcctc ggtaaaaacc gtaaaacggc ggtgttcccg aaactggtgt ttgcgattcg
    14401 cgatggcctg aaccataaaa aaggcgatcc gaactacgac atcaaacagc tggcgctgga
    14461 gtgcgcaagc aagcgcatgt atccggatat cctgaactac gatcaggtag tgaaagtcac
    14521 cggttcgttt aaaaccccga tgggctgccg cagcttcctc ggcgtgtggg aaaatgaaaa
    14581 cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg atcagcctga acctgccgcg
    14641 tattgctctg gaggcaaaag gcgatgaagc caccttctgg aagctgctgg atgaacgtct
    14701 ggtgctggca cgtaaggcgt tgatgacccg tatcgctcgt ctcgaaggcg tgaaagcgcg
    14761 cgtggccccg atcctctata tggaaggtgc ttgtggcgtg cgtctgaatg ctgacgacga
    14821 cgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg ctgggttaca tcggcatcca
    14881 cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac gacaacgagc agctgcgcgc
    14941 gaaaggtatt gcgattgttg agcgtctgcg tcaggcggtg gatcagtgga aagaagaaac
    15001 gggttatggt ttcagtctct acagcacgcc aagtgaaaac ctgtgcgacc gcttctgccg
    15061 tctcgatacc gctgagtttg gcgtggtgcc gggcgtgacc gataaaggtt actacaccaa
    15121 cagtttccac cttgatgtgg agaaaaaggt gaacccgtat gacaagatcg actttgaagc
    15181 gccttacccg ccgctggcga acggtggttt catttgctac ggcgagtatc caaacattca
    15241 gcacaacctg aaggcgctgg aagatgtctg ggattacagc tatcagcatg taccgtatta
    15301 cggcaccaat acgccgatcg atgagtgcta cgagtgtggc tttaccggtg agttcgagtg
    15361 caccagcaaa ggcttcactt gtccgaaatg tggtaaccat gacgcctccc gtgtgtcggt
    15421 gactcgccgc gtgtgcggat atttaggtag cccggatgca cgtccattta acgccggtaa
    15481 gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat gggcagatag gttaatcttc
    15541 actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct tatccggcct acatttggca
    15601 acggctgtag gcctgataag acgcgacagc gtcgcatcag gcgttgatta ctggatgcag
    15661 cctacacgcg gactgataag ccaggcgaaa taatgaatta tcatcagtac tatcctgtcg
    15721 acatcgtcaa cggccccggc actcgctgca ccctgtttgt ctccgggtgt gttcatgaat
    15781 gccccggttg ctataacaaa agcacctggc gggtaaattc cggtcagcca tttaccaaag
    15841 caatggaaga ccagatcatt aacgatctga atgacactcg catcaaacgc cagggtattt
    15901 ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc ggatattctg aaactggtac
    15961 aacgcatccg cgccgaatgt ccgggtaaag acatctgggt gtggacaggc tataaactcg
    16021 acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat taacgtgctg gtcgacggca
    16081 aatttgtgca ggatttaaaa gacccttccc tgatctggcg cggcagcagc aatcaggtgg
    16141 tgcatcattt gcgatgataa gttccctgtt acctgagtgc aggcaacagg gaaataagga
    16201 attaacgata cttctggtga taggcgttgc gggtcgtttt cagttgctct gcggcagcct
    16261 gcgcttcttt tactttacct tcatttgcca gcttcagcgc gtcgtcaatc tgaccgacca
    16321 gaatatcgaa accgtggcgg aagtctttca tttccgggct gtccggtgat ttgccttcga
    16381 gcttcggcgg cgtcgctttt tgcgcatcca gcgctgcggc gcgcatcttc gttaacgcgt
    16441 ctttgacttg cgccgcgtta tcggcttttt cgaccacttt taaattgtcg ttgagggttt
    16501 ccatattgtc ttcaagatca gcggcaaacg acgccgaact gaataccaac gaggagactg
    16561 caagaatagc taacaggctt ttacgcattg ctcacttcct tttttattta cacttcatcc
    16621 ttcacactgc ctcatcgacg gctacgctca ttcgcccgag tcacgtactg cgagtacgct
    16681 ccccggtcct cattcacttt ccgtctcgat gcagtgcgaa tgattttgtg taatttttta
    16741 gtttatagcg cggcaggtcg cgccagtttt ttactgcccg gcgattttca tctccggcaa
    16801 cagcacagaa ccacactgta tattactgcg tgtttcaata tcgttaccga cggtgacaat
    16861 attgcgccac atatctttta aattacctgc gatggtgatt tcgctaaccg gatactgaat
    16921 ttcgccattc tctacccaga agcccgctgc accacgggaa taatcaccgg taatggcact
    16981 cacgccctgg cccatcagct ccgtcaccac cagcccggtg cccatctctt tgagcatttg
    17041 ttcgaagctt agcccttgtc cggcaatccg ccagttgtga ataccgcccg catgtccggt
    17101 gcttttcagc cccagtttcc gcgccgaata gctggtcagc agccactgag tcaggatgcc
    17161 atctttaata atatcgcgac gctcggtgcg cacaccttcg ctgtcgaatg gcgtagaggc
    17221 cagccctttc agcagatgcg gatgctcttc aatggtcagc cagtccggca gaatttgttt
    17281 acccagcgaa tccagcagga aggtagattt acggtaaacc gatccacccg ctatcgcccc
    17341 aaccagatgc ccaaaaagcc cggttgccac ttcattggca aaaatgaccg gcgctttcat
    17401 ggtggagagt ttacgcggtg acagacgcga taaagtgcgg cgagcacagt cggccccgac
    17461 ccactctggc gtttgcagat cgctcatcgc acgaccaatg gtgtaggcgt aatcacgctc
    17521 catatcgcca ttttcttcgg caattacaca gctggagagc gaatgacgcg tagagcagta
    17581 accctgcaac atgccgtggc tgttgccaaa aactttgaca ccgtagtggc tgttaaagct
    17641 gccaccttcg gtattggtga tgcgtttgtc cgcctgcaat gctgcctgtt ctgcgcgggc
    17701 cgccagttca atggcttcat ccggggaaac ttccgcaggg tggaacaagt cgagatccgg
    17761 tgcgtcaaag gccagcagct ctttgtctgc cacgccggca cagggatctg gcgaggtata
    17821 acgggcaata tctagcgccg cctgtacggt gcgggcaatg gcctgcgggc ttaaatcggt
    17881 agacgatgcg ctacctttgc ggttctggtg ataaacagtg atccccaacg cgccatcgct
    17941 attgaattca acattctcca cttcaccata acgcgtgctt acgctaatgc cggtggtctt
    18001 gctgacggca acttctgcac cgtccgattt gcctgaggcc aactccagcg cagtcgaaac
    18061 tgcttcttcc agaatcttgc gctgcgcttc aacttgagag attactttca ttgcaagtgc
    18121 cataatgtag agagtttctc tgaagtctaa cagagaaccg tttttcagtg cgcatcttaa
    18181 ctggtaacat tagcctcttt ttttaaggag cctgagatga ctaagcagcc cgaagactgg
    18241 ctcgacgacg ttcccggtga tgacatcgaa gacgaagacg atgaaattat ctgggtcagt
    18301 aaaagtgaaa ttaaacgcga tgccgaggag ctaaaacgcc ttggcgcgga aattgttgat
    18361 ctggggaaaa acgcgctgga taagatcccg ttagatgcgg atctgcgcgc ggctattgag
    18421 ctcgcccagc gtattaagat ggaaggtcgc cgccgccagt tgcagctcat cggtaagatg
    18481 ctgcgccagc gcgatgttga gcctattcgt caggcgctgg ataagctgaa aaaccgccac
    18541 aaccagcaag tggtgttgtt ccataaactg gaaaacctgc gcgatcgtct gatcgagcaa
    18601 ggtgatgacg ccatcgctga agtattgaat ctgtggcccg atgccgatcg tcagcaactg
    18661 cgtactctga tccgtaacgc gaagaaagag aaagaaggga ataagccgcc gaaatccgcg
    18721 cgccagattt tccagtatct gcgtgagtta gcggaaaacg aagggtaatt ttcgttgttg
    18781 atgtgagtat ggtttgccgg atgcggtgca cctgtaggcc tgataagacg catcaagcgt
    18841 cgcatcaggc actatgctca aatgccggat gcggcgtgaa cgccttatcc ggcctacaca
    18901 cactctatca ggctgaggct gtgaattact gcgcggcttc cgctttcttc gctaacccat
    18961 ccagcagttt ctgatggatc ccaccaaaac cgccgttact catcaccaga atgtgatcgc
    19021 ccggctgagc ggttttcacc accatatctg ccagcgtatc cacatcgccg ctccagtgtg
    19081 caggctgaac gcaggcttct gccacttctg ccacctgcca cggaatatgc gccggttgca
    19141 gcaggaagac ttcatcggca cgacctaatg aaggtgccag atcgtctttg cagatcccca
    19201 ttttcatggt gtttgagcgc ggttccagca cggcaataat gcgcgccgta ccaccaactt
    19261 tgccacgcag cgccgcaagc gttgccagaa tcgccgtcgg gtgatgggca aaatcgtcat
    19321 ataccgtcac accattcgct tcaccacgca actccagacg gcgacgagca ttaataaacg
    19381 aacccagcgc gttagcggca tctgccggcg ctacaccaac atggcgagcc gccgcaatcg
    19441 ccatcaggcc attgtgcata ttatgttcgc ctaccagcga ccatttcact tcgcccactt
    19501 tttcgccatc cagcaagact tcccattcgg aagcatcggt ggtcagcttt ttcgcctgcc
    19561 agtgaccttg ctcgcccacc agctcctgct cgctccagca gcccatcgcc atggtctgtt
    19621 tcaggttgat gtcattttcc ggccagataa tacggccctg ccccggaacg atacgcacca
    19681 gatggtggaa ctgtttctgg atcgctttca ggtcgtcaaa gatatcggcg tgatcgaact
    19741 caaggttgtt gaggatcagc gtacgcgggc agtaatggac aaatttagag cgtttgtcga
    19801 agaaggcgca gtcatactca tccgcttcga taacaaagaa gtcgctttcg cccagacgcg
    19861 ccgaaacctc aaagttcccc ggcacaccgc cgatcacaaa tcccggtttg taaccgcact
    19921 gttccagaat ccaggttgcc attcccgcgg tggtggtttt gccatgtgta ccggcaacgg
    19981 ccagcaccca gcggtcgcgc agcacaaaat cgtgcagcca ctgcggacct gacatataag
    20041 ggatgttttt ttccagtacc gcttccacac acggatttcc acgggtcatg gcgttaccaa
    20101 taatcaccag atccggctgt ggatcgagct ggctggcatc gtaaccctga atcagttcaa
    20161 tgccttgctt ctcaagtaag gtgctcatcg gcggatacac attggcgtcc gaacccgtta
    20221 cttcatggcc taactggcgc gccagcatcg ccagaccgcc cataaacgtg ccacaaattc
    20281 ctaaaatatg aatgcgcata cgtcactatc cttttttaat ctggcgctca ttttacgcat
    20341 atgtcgggca agtgagaaac gcatttcagg ataatccgta atttgctggc gcgattcacc
    20401 tgagcgcaac attgggatta tttgttaaga ttgttgcggt cgctttactc cataaacatt
    20461 gcagggaaag ttttatgaaa acgttaggtg aatttattgt cgaaaagcag cacgagtttt
    20521 ctcatgctac cggtgagctc actgctttgc tgtcggcaat aaaactgggc gccaagatta
    20581 tccatcgcga tatcaacaaa gcaggactgg ttgatatcct gggtgccagc ggtgctgaga
    20641 acgtgcaggg cgaggttcag cagaaactcg acctgttcgc taatgagaaa ctgaaagccg
    20701 cactgaaagc acgcgatatc gttgcgggca ttgcctctga agaagaagat gagattgtcg
    20761 tctttgaagg ctgtgaacac gcaaaatacg tggtgctgat ggacccactg gatggctcgt
    20821 ccaacatcga tgttaacgtc tctgtcggta ccattttctc catctaccgc cgcgttacgc
    20881 ctgttggcac gccggtaacg gaagaagatt tcctccagcc tggtaacaaa caggttgcgg
    20941 caggttacgt ggtatacggc tcctctacta tgctggttta caccaccgga tgcggtgttc
    21001 acgcctttac ttatgatcct tcgctgggtg ttttctgcct gtgccaggaa cggatgcgct
    21061 tcccggagaa aggcaaaacc tactccatca acgaaggaaa ctacattaag tttccgaacg
    21121 gggtgaagaa gtacattaaa ttctgccagg aagaagataa atccaccaac cgcccgtata
    21181 cctcacgtta tatcggttca ctggtcgcgg atttccaccg taacctgctg aaaggcggta
    21241 tttatctcta cccaagcacc gccagccacc cggacggtaa actgcgttta ctgtatgagt
    21301 gcaacccgat ggcattcctg gcggaacaag caggcggtaa agcgagcgat ggcaaagagc
    21361 gtattctgga tatcatcccg gaaaccctgc accagcgccg ttcattcttt gtcggcaacg
    21421 accatatggt tgaagatgtc gaacgcttta tccgtgagtt cccggacgcg taatctcctg
    21481 cgcctctcca cacggagagg cgtttttatc gctgcgcaat gttgactctt gtcacaggcg
    21541 aactctgacg attctcccac agcaccgtta atccacgctg taatgctata aaaataaaca
    21601 acaaaatacc gatggcgatt ttcgtccacc atgagctcag cgtgccatca aagtttatgt
    21661 aagtctgaat cagtccctga atcgccacgc caaaaagcgt ccctaatacc gttccaacgc
    21721 caccactcaa aagcgtaccg ccaattacca ctgaggcgat agcatccagt tccacaccta
    21781 cgcccgccag cgcatatccg gcctgggtat aaatcgagaa gacaatcccc gccagtgtcg
    21841 ccagcccggt agagagcata taaatgcgaa tagtggtgct gcgagtggaa atccccatca
    21901 ggttcgccga cgttgcgttg ccgccaatgg cgtacacctg attaccaaaa cgcgtacgat
    21961 gcgcgaggaa tatgccgata accaccaccg ccagcatcag cagccccatc gcacttaagc
    22021 gaccgccgcc agggattttc cacgcaaggc ttgagagcgt gtcataaaca ggatggttta
    22081 tcgggatcga ctcttccgaa acgagatagc tgacgccacg caaaaagaac attcctgcca
    22141 aagtaatgat aaacgccggg atcttcaggg cgtcaatcag aagccccata aatgcgccga
    22201 aggcacagcc catcaccaac accagcggaa acgccagcag cggcgagagg ccgaaatcgc
    22261 caatcacttt tgccagaaac acgccggtaa aggcgatcac cgaaccgacg gagagatcga
    22321 tcccaccgga gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcattat
    22381 cggtcaggat attgcagatc actcttgtgg aagcaaaacc gggaaactgg gtcagacagt
    22441 agatataacc caacacaaag acgccgatgg tgatcatcag tggcaaatta cgttttatca
    22501 cggttacgta cccctttaat cagactgata aagcgttgcg actggacaat cagcacgcaa
    22561 agcaccacca ccgctttcac cacctggttc atctccggcg gaaatccaga aagcaaaatt
    22621 ccggtgttca ttccctgaat aatcagcgcc cccaccaccg aaagcagcag gttaaagcgc
    22681 ccgcccatca gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat
    22741 aacccggcgt tattggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc
    22801 gccgcacata gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg
    22861 gcattttttg ccgcccgaat attgatacca acggcttcga taaacatccc cagtgccgtt
    22921 ttgcgggtca acagccagaa caggagaagc gtcagcaccg caataatgac tggcgttggc
    22981 aggaacaaca gcgatccact gccgaaccag gagagatccg gtgagttaaa tgtgacgatc
    23041 tgtccggagg tgatcagttg cgccacaccg cgcccggcga ccatcaggat cagggtggcg
    23101 acaaacggct gaattttgag gatcgctacc agtatgccgt tccacaatcc tgccaggatg
    23161 ccagtgccca gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca
    23221 gccgttgtgg ctccggcgat agccatcacc gcccctacgg agagatcaat cccaccggtg
    23281 gcgataacca gcgtcatgcc aatcgccagt agcgcaacgg gggccgcacg gttaagaatg
    23341 tcgatagggc taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggcc
    23401 accaggctat cgaccagcag caccagcaac agtgccgcca gctgcggcat tcctgttggc
    23461 cagcgaaaac gccttttcgg cggcgtagtg tccgggagag attgaggcat cacactattc
    23521 tccttacgcc gcaatggcgt tcatgatcgc cggaacggaa agctctgcca gcgggatctc
    23581 cgccacctgt ttgcgatcgc gcatgataat cacccggtcg gcatagccca ccagctcttc
    23641 cagttcagag gagatcacca gcagcgccag accgtcagcg cacagcgttt caatcaggcg
    23701 gatgatttca gcgtgggcac caacatcaat gccgcgggtt ggctcgtcga ggatcaaaaa
    23761 ttgcggacga gtcagtagcc aacgtgaaag caacactttt tgctgattgc cgccagagag
    23821 aaattcaatc ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc
    23881 ggcaatctct tgctgttctt tgcgggaaat cggtcgtagc cagccgcgct gggcctggag
    23941 agcgaggatg atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc
    24001 ttccgggcag aagccgatac ccagtaccga agcctgatgt ggcgatcgca gggtttgctg
    24061 tttgcctttg atcaacgccg tgccgctgtc agcaggtttg atcccgaaga tcacttcggc
    24121 ggtttcggta cgtccggatc ccagcaagcc tgccaggcca acgatctcgc cggggcgtac
    24181 ttcgagatca aacggtgcga tcgttccttt tttgccgtaa tttttgaacg cggcaacggg
    24241 tttgtcgctc agcaatgttc tcccggcacg ctgaagcgcg tgggtgtcca gttcacgccc
    24301 cagcatcatt tttaccagtt cgatctgcgg tagctcgcgg gtttcccgac agcccacaaa
    24361 actgccgtta cgtaagacgg tgatccgatc gctaacctga taaacctgat cgagaaaatg
    24421 agtgacgaag atcaggctga cgccgcgatc gcgaagctga cgcatcaggc caaacagtaa
    24481 ctctacttcc tgagtgtcga gactggcggt gggttcatcg aggatcagca ctttggcgga
    24541 gagatcgata gcccggcaaa tggcgacgat ttgctgcatc gcgactgaaa agcggttgag
    24601 cggttcgcgc acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc
    24661 catctcttta cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc
    24721 agcgaccgac atattgggta gcaggttgac ttcctgatag acggtgccga tgccgagttg
    24781 ttgtgcgtgg gcggtgtttt tcggtgagat agcctggcct tccagccaga tagtgccgcg
    24841 atcggcgtgg tatacaccgg ttaatgcttt gattagcgtc gattttcccg ccccgttttc
    24901 accgagcagc gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt
    24961 gacaccgggg aaaaatttac ttaatccttc ggtgcggagg atttcctggt gttggtcggt
    25021 cgtcatggtt tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc
    25081 gtcgcatcag gcttcagcgc acaaccgccg gatgcggcgc gagcgcctta tccggcctac
    25141 gaatcggcat caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt
    25201 cttttgcggt atcaggcaga taaagggtgg atttggttaa cgtcagcttt tcaggcatgg
    25261 tgccgtcttt tttatatttc tccagcgcgt cgaaggcggg acctgccata ttcggcgtca
    25321 gttcaacact ggcgttcgct tcgccatcaa tcatcgcttt gtagatgtcc ggcacaccgt
    25381 cgatagagcc tgtcaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa
    25441 ttgcaccaat caccatgtca tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt
    25501 tttccgcttt gataaagctc tccatcactt ctttgccttt gctgcgggtg aagtcacctg
    25561 actgcgagcg gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct
    25621 tacggtcaat ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt
    25681 tgccattcac ttcttttacc agccagtcac caatcaactt gccttcgagg atgttgtcgg
    25741 cggtgacagt ggtcatatag agagatttgt ctttcacatc gatggaacgg tcaagcaaga
    25801 agaccgggat ttcggcatct ttcgcctctt ttaataccgg ctcccaaccg gtcgctacca
    25861 ctggagcgat aaagatcgca tccacccctt gcgcgacgaa ggaacgtacc gctttaatct
    25921 ggttttcctg cttttgctga ccatcggcaa ttttcaacgt aattccgcgc ttttcggctt
    25981 cacttttcgc cacattggtt tctgcggcgc gccagccgga ttccgacccg acctgcgaaa
    26041 atccaacggt taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga
    26101 ctacaagtaa gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa
    26161 gatgttcgcg aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt
    26221 caaaaaagta aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa
    26281 atgcggctac ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg
    26341 aaaatgaaga cgaaaacaag cgaagacatt cggcgcgagt tggctataat actcggcact
    26401 tgtttgccac atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa
    26461 agatctgccg gaagacatct acgttgttat tgagatcccg gctaacgcag atccaatcaa
    26521 atacgaaatc gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat
    26581 gttctatccg tgcaactacg gttacatcaa ccacactctg tctctggacg gtgacccggt
    26641 tgacgtactg gtcccgactc cgtacccgct gcagccgggt tctgtgatcc gctgccgtcc
    26701 ggttggcgta ctgaaaatga ccgacgaagc cggtgaagat gcgaaactga ttgctgttcc
    26761 gcacaccaag ctgagcaaag aatacgatca cattaaagac gttaacgatc tgccagagct
    26821 gctgaaagcg caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg
    26881 ggttaaagtt gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt
    26941 cgagcgcgcc aagaataaat aagttcttct ggcgcaataa ccctgaacac cgggcttcgg
    27001 ttagtaaggg tttttttatg cccgcaattt ataaactctc tactccacta ttattatttt
    27061 cagcagttga aaggcttgaa cggtaagaac aagctaaact cgaccaccat tttgctgttc
    27121 atagccactt gctggaagtt agccggcctc actcatactc accgttcaaa gcgtgtcccc
    27181 gttttacccc tgtacatttg ttaacgccag cagagttttt tatgcgattg cagatatgga
    27241 aagcggcccg gagagaaaag caaaagatgg gaaagttaca aaaaaaatcg cgagatacat
    27301 cgcataaaac aacgccacct tttcggtggc gtttttatcg cccacgcacc accagtgcct
    27361 ggtccagctc gcgatacgct tcaaccagtt tctccagtga aacgcgactt aaaccgctgg
    27421 gatttggcag cacccaaatc tgcgtcgaac caatggtgag agtttgtttc ccccactgtg
    27481 caccgcgctg gctgaatccc tgttcatatg cttgtttgcc cagaatcgcc aacgcctgcg
    27541 gctgataatc ttcaattttt tcaatcagct tacgcccgcc tgcgtgtagc tcctgctttg
    27601 aaacttcatt ggcttgcacc gttggacggt ctaccagttt ggtgacgcca caacgataat
    27661 ccagcagatg ctgtgcctcc tgcggcttca actgacggtc ggtaaacccg gcctgatata
    27721 tcaccttcca gaagcgattt gccggatgag caaagggaaa accagtcccg gcggatgaaa
    27781 gcccagggtt gataccgcaa aacacgaccc gtaaccctgg agccaaaata tcctcaacca
    27841 tatttactcc tttctaacat caccaggaaa agtataacgg attgaaaata cattgtttat
    27901 aaaaacagca ggcgcgcggt aatggctgga ttgcgacacg gagttacttt ataatccgct
    27961 accatggccc cttagctcag tggttagagc aggcgactca taatcgcttg gtcgttggtt
    28021 caaacccaac aggggccacc atatacaaca aagggttagc tagtaaatca gctaaccctt
    28081 tttcttttgc ctttgaattg ggtcaggtaa tgggtcaggt aagctttttc tccatctgac
    28141 tcagatggct gatagttgcc ccggataatg cttcgcaatg atgtcattta gccgatcaac
    28201 cagttctggt tttctgaacg tgatatgcgc ggagcccttc ttaaagtatc tgatactgaa
    28261 catctcatct tcatagtcct ctttaccctg aacagactgg atgtgatcat ccagatgaat
    28321 ggtgatgttt tcccggttgt cggggatcgg tttgccgctg aacaggtgta gcattctttc
    28381 cagatcggca agtcgatcgg cctgttgccc gctgttcaga tgaaatcccc acctgtccca
    28441 cctcaccaga ttgttgacga taattttact gccaaattta cagggtgagt tggttttgta
    28501 atcccagctc agtcccctga agacgttgat cacccctcgc tcaaacacct cattcttatt
    28561 ctggtgcagt tgttcaaatg tgctcagaat gttcgcttca ctgatttccg gaaaattatc
    28621 gtactccagt gaccggtacc atgtatcacg ggcctgtgca tccattaagg aaagcatccc
    28681 tgaacgttgc atcaggtcac gccagatttc tcgatcaaga ttgcgggtga tggctttcat
    28741 tgccgtttca ggtttttcca tcagccagca accgcagcga aaatcctgtt tcattgccca
    28801 gtccagtgcg gttttaccgc caatactttc tgtcattgct gatatttcag ccagttgccg
    28861 gataagtgtg tcgatttgca gcagtgtact ctttcgtaca gccagaatgt ggcggatact
    28921 ggttgagcag ataacgtccg tatgcccggt gaccagttcc ggacgggaga ttgtgatgtc
    28981 tgacattatc ggtttccctt tgggtttata aacgaaaacg ccagccctgt tcaggctgac
    29041 gtcagtggaa tgaagtctgg tgagtgagat tcaccggttc tggtgcagaa gctgcagctg
    29101 gcgcagggta cggatatcct ccctggcctg ttcaatacag ggcaacagtg ctgccgaatc
    29161 cgttttatct tcatcgttgt caaagataat tcccgattcg cagtcgatat tgtcctgcag
    29221 ccatgcaatc agaatatcca gcgctgtttc cgtggttaat gatttcatgt tgtttctggt
    29281 taccagtcga aagtgggtaa acccggcaga catccggcac tggcatccag atgaatgaga
    29341 ctgacgccat aacgtcgggt aagtgtgaca accagacggc ggaacgtttt tgacaggccc
    29401 aggcgcttaa gccgcagaac cgggtatgac cacgcatcgg tacgtaacag atagccgcta
    29461 cctgtgaaat gaatccattc tgattcacca acgtcactgg tctggtgtga cagcgagtac
    29521 aaccaggcgt tgtccgtttc cgtgatatgt gcggtactgc agcgtatgcc ggcaagggtt
    29581 gcaaatggcg gaggtgtgca ggttgactgt tggtcggatt cctccaccac acggagtgaa
    29641 taaccgtttt cagcgacgtt attaatcagt tcagtgagat taacaccatc gacgtcaacg
    29701 acaatgcgcc ccatgttcag tgcctgcacg ttaacgctgt cagcttccag cgtcagggcc
    29761 agtttcatcg tttcgcctcc tgatacttac ccagggtaat gttatttacc gttcggtaat
    29821 tgtcgcgggt catcaggcca gtcacccggc gagcccggag gatatcgatg ctgtttatta
    29881 actgagagcg ggtacaggca ctgaattccg gctggtcggt acgcaccagc gcgtattttt
    29941 caacaagaaa gttcactgca tcgcacagtg aaatgccggc ctcaatatgc tgctcaatca
    30001 cacgttcatc agcgaacggt gtgtcattca gcgtaaggcc atagtgctgg tccagcagtc
    30061 gggtgagcag tgtctgccag atttcaacag gagacgggcg agaactggcc gcctgcccgg
    30121 gtaatacagg taatgttttc atactgaaga ttttcctgat atgcagatat aaaaatggaa
    30181 aagtggcgtg gtgaaaacac cgggccgtag cagaaggcta ttctggagag ttaatttttc
    30241 atttcgggcg tcggataaac agccagataa acgtaaccac aggagccgag ggtgtcggct
    30301 tcacaggtca gccccttcgc atacagtgtc acggtatgct gatggcgggg attcagttca
    30361 ccgctggtga gcatgagctc cagttgtttc atcagcagcg gaaaggcctg gtccagatgg
    30421 tacgcatccg cgtcgctgaa ccggcctctg ataccggcgc ggtcagcaag gtaatgcaac
    30481 cggttaccct cctgtaccag tcgggccccg aaacagggcg tcacggtgca gggcagcccc
    30541 caccaggggc ggtcgtgatt gtcgtcggga tgcgttgtcc cggggagtgt gtctgacacg
    30601 ataaagtcct cacaaaaaat cggttaagaa tggtccggtg gtggcgataa ttctgctcat
    30661 cagaatgccc tgattcagtt cagggtgacg ctcatcagcc ggacatacgg gccaaaactg
    30721 tccttatggc gttcagcaaa cacggccagc acaccgggaa tatcctgcac ttcacgaccg
    30781 gtatacgccc cggcactgcc gtgccagcgg tacttgccgg tgcagaacgg aaaaagacgg
    30841 gatgtcggat gttgttggtg aatacgcatg gcttcaccac gggtgacgat ttttatagtg
    30901 tgatacctct gaagacagaa gataaaagcg aaaacaggta tgatgtggtt gtgacggtga
    30961 caggttaaag cagaccgtgt tccgcaaagg agaaaacctg gctgccaccg actatcagat
    31021 ggtccggcac ccggatgtca accagggcca gcgcctgtac cagacgttcg gtgataagcc
    31081 ggtctgcctt actgggtgtg acttcaccgg acgggtgatt gtgtgccagc accacggcag
    31141 cggcattgtg gtacagggcg cgtttaatca cttcccgggg atggacttcc gtgcggttga
    31201 tggtgccggt aaagagggtt tcaccggcaa tcagctgatt ctggttgttc agatacagca
    31261 cccggaattc ttcacgctcc agtcccgcca tgttcagaat cagccattcc cgtgccgctc
    31321 gggtggaggt gaaggccacg ccgggttcat gaagatggcg gtccagggtt ttcagggccc
    31381 gcagaatgag gctgagctcg ccgggcgtca tcgcgccggg cagaaaggaa agttgttgca
    31441 ttgtacttct cctgctcagt cgatgatgcg cataatggcg ctgcattccg gatgctgcag
    31501 ggcgtaatcc cgcagccggt aataatggac cgtcatggca taacactccg tacgacaggc
    31561 gtgatgactg tacgtcatca gacaggcggc aatgccggcg gcttccgggc tcatttcagc
    31621 gcggttaccg ttcatggcat tgaacagtac ccatgtttcg tcatcatcgt catccggttc
    31681 gggtgccata aatgccccgc cgttgttcag ggtatacaga ttccagatac caccacagta
    31741 gtcttcgcaa agacggtcca tccagccgaa aacacggggc tccagggtca cccactgtgg
    31801 aatgaggcca aaatgctgcg gccagaagct gatgcgctgt tcatcaggga ctatggtggc
    31861 aaccagttgt tgcaggccag tctccggtgc acaggctgaa gaggtggtgt tctgagacaa
    31921 tgttttcatg tgtgaaatcc ttgtaatgtg aataaatgag aggaaataaa ggccgggggc
    31981 tgacaacatc acgttcatca ctgaccgtga gacccggcaa tatcagggat gaatgatatt
    32041 cggctgaaat tccggacagc cgttaaccgt tccggtcaca gacggatggc cagatgccat
    32101 tgcctgtact cagtctgcac agactgagtt ctccgggacg gtaaagcaga cgggtacacc
    32161 aggcgcacag gtcatcatca gaaatggaac gacatcccgg cagcgcctcc ggggcgtcgg
    32221 gtgatacggg tgcctgaatc acgccttcag ggatatgttt catcagcgca tgctctccag
    32281 cagcgtttct gccatcaccc acaatgcgcg gttgagctta atatcggtgt cgatgctgtg
    32341 aatggcacgg gtgtggatac gttttccttt tgcactgcga ccggaaatcc cgcctttcag
    32401 catattctcc tggatggtct gatatgcgct ccacaggtct ttaccgtaat cctcccgacg
    32461 tcgtggcgtc agaatgtcgg cggtggtgac gggctgatgt tcgtcaccat aacggtaagt
    32521 cagtgccgcc tgtgccagcg cctggcgtgc cggtggcggc aggaccagcg actgcatggc
    32581 atcacgtttc tcttcaatcc ggtcaaaaac gcccaccacc tcgtaagccc cttcaatgac
    32641 tttctgcacc acatttcccc ggtgtggaac acgcacttcc cccagagact gaccacagac
    32701 gcacccgttc tggcagacga accggaagta acccggcagc atctggtagc tggaggtacc
    32761 gtcatgggag ttgagcagaa taatttcagg gacatgttct ccgtttatct ctccggcccg
    32821 ccgcagacgc agcatgtgtt ttgtgtatcc ccggcggccc gggtcgcgca cacgggtctg
    32881 gcaggcgaaa aagggctgga agccttcccg ctgcagactt tccagtacgg tgatggtggg
    32941 gatgtacgta tagttctggc ttcgggatgt gtgtttctct tccccgaaaa tccccggcac
    33001 atggtgcatc agctcttcat gtgtcagcgg acggtcacgg cgtatctggt ttgcagcata
    33061 accaaaacga ctgactaatc gcatactttg ctccttatcg gtggttaaga tttactggtg
    33121 caataaatga aaaagccgcg tctcccggag aagacgcggc ctgatggacg aaatcaatga
    33181 cgtttattgt ctgagaagcc ctccactggc gagccacgca ttaagctgtt ttccggcatc
    33241 agcttcagag ttccggcagc gtcagcgtcg ctgaccttcc gcgttgcgga taacccgacg
    33301 gaaatgcatt cccgggtcat cctggataaa cacattgcgg taagcggtta tgacagcaac
    33361 cgcctgcccg tatgagaaag atccttcagc caggacatac tctgtgtgta acccggcata
    33421 tctggtttct cctgataaat agcctctgcc atacgttgtg gcagaggctg aagcatgaaa
    33481 ctgacttcag ggatcagtta acatttttcc ggaaacggta atcagcagtg gatggtagtc
    33541 ctggggatcg aaaaccgata acggcagact gacacgatgg ccgttacttt cttcagttgc
    33601 tttaatgatt tcggttgtgg cgacattttc cacgcactcc gtttccagaa atgcgtctgt
    33661 ggttcgcgtg gcattactgt caccaaaggc ttccgtttcc atttttctgg tcaccagcgt
    33721 ctgaccatat ttgtctttga gttgcagagt gatggtgagg gggccaaatc cttcatcgtt
    33781 tccgccatta tccagccgga actggtaagc acaaatattt cccgggagcc atatcgtatc
    33841 tgtattgcgt atactgatgt aacgttgatc ctgtgcccgg agtggggcag accacgttaa
    33901 ccccagaatg aaggcggtaa tcatgcaggt tttgaacagg tgaatcatgg tatttacctc
    33961 tctgagtcat gacgatgaca ctgacaaatc aggtgataaa acgtaaaagg cgcagaatag
    34021 ccgttatgcc ggtaactccg ggagtaatgc ttcttcctgt cggttaacca tcttgccgag
    34081 atgggatgca tcatattcca tgacggggcg ttgcctgatg atactgacca ccagtggttt
    34141 gattaacata ttggtcgcgg cccgttgttg tataccggcg gcgaaaatga tctccagagc
    34201 attctgatcg ataacacggg ccagattcgc gttcagattt tcaatctcat gtccggtcag
    34261 caggaaactg ccggcaagcc atgtcacctg ctcctgaatc attgccagaa ttttgacgcg
    34321 atcttcttca accagcgctc cggcaatttt tcctgataca gcggaggcaa tcattccccc
    34381 cagaacaccg ccagccacac gccctgtcag tgcaccaaat ggtcccagtg gactgaacag
    34441 tatcccgccc gccactgacc cgacagcacc acctgccaca ccggaagatg ccacggcaag
    34501 attcctgatg aactgcgcac cggagatccg tccccgcaac attttgatca tgtcagggcc
    34561 ggttgtgaca gcgaccagta ccagagatgt caccagcgta cccttcatca ctttgttcag
    34621 ggcactgata tttcctgcac cggtcccctt ctgaagcgcc tgccggacag tcgggctggc
    34681 cgtcgaaaaa tcaatatgct tcagcatacc ctgaacaaca ctgagccggt ggagttgctg
    34741 ggttgtgacg tagacagcca gtgtgcgggt gaaggttttt cctgcctgga cagcagctgt
    34801 ctgcagggta gcatcgcgat cgccggtgct gagccagaag accgatgccg tcagggcaaa
    34861 actgatccct ccggccgcca gactgacaac cgaaccctcg gcaatatcat aagtgaccga
    34921 ttcgatggtc ccgaaccggg tgatattacg ggcctgagta taagtcagat gtccccgacg
    34981 aatcagccgg gacgcttctg cgggatcggt tacaccgggt actttacctt cgcggatctt
    35041 attcctcatg gtttcaacag cgccggcata ctgatcacgg gggacttcca gttgcatggg
    35101 gccattattt cccatgtaac gatactgtcc gttttgtccg tcgaatgccg caccgacgct
    35161 acgggctgca gttgaacaat atttcgtctg gatttctgtt ccgctgacca gtctgtctgc
    35221 tccatttctg gcattattat caccgaggat ccgtgcgttt ttcagacgga ctctgtcgac
    35281 aatatggttt ccccgttcag cggcaaaccc atgtccctgc cctccggaag caaacatttt
    35341 ctcatttgta ctccaggttt ccggcagttt catgagaatc agaccattcg ccgttgcatc
    35401 ggaaataaag ctgttgtaag ggcgttcata aatttcctga cacagtgttt ctgtttcgcg
    35461 acaaagagag gagcagtacc attccggatc cggcttatga ttttccgggg catgatgtac
    35521 ttctgtcagc ccgcaggagg cacagatacg aagctcatcc acgtgatgga atgatacaaa
    35581 acggcgacct tctgaaaagg gataccagtc accgccgttc aggcaacgat cgagaaaatc
    35641 gtcattcgct tccggtgata aattcaggcg gttccggaag agcgttttca gttcatcagg
    35701 tgtgaatgac aggttctctt tcaactgttc gttaaaccct gccatgtttc tttcatacca
    35761 ggcattgttc agtaaccagt ttccgtctgc cagttcaaat tccctcagct ctccccggga
    35821 aatccgttga gaaatgagcg tcagccagat gcgctccgtc agtgagtcag gaaggctgta
    35881 atcgttttgc aaactggtca gccacgattt actgttgagc accggttgtt cttccagtgt
    35941 ctggctgatg gcacaggtca gttttttcag cgcctggatc tggctggccg cccatagcag
    36001 cgtcactaat gcagccagcc agtacaacaa cccgccccac cacatcagtg acggtgaaat
    36061 atcgtactgt aacgcgatca atggtgcgca tgcagaacac gccataagga aaaaggcagt
    36121 ataaaaacag cagtaatacc cgttaatttc cttgctgata tcttctggtg tgatcccgtt
    36181 ctgctgaatg tttttgatct gcaggggaac agccagcaga ccaccggcga gagcggtggt
    36241 cagaataaat ttcacagagt caaaggtgtg agcctccagc gcgatatgtt taccatcaag
    36301 tccccacatg aagctgatga tcattccggc aatcgtcaga aatacgcctg ccagccagcg
    36361 aatatggaac aaccgggtta tgcgttcggt gatatagcca gccagtaagg ccgggccgaa
    36421 cagacacagc aatatgagcc ctgccagcgc agaagccgtg ctgattaatg ctctgaacaa
    36481 aaggcgaaga atggcaaatc ctgcgatcag caggatcagc gcgccgacta tctgtaacat
    36541 agtcactccg tgatgaatat catgttattg ttaatgccag cgaatgtggc gttgaag
//
LOCUS       DAZGHF010000033        36174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGHF010000033 DAZGHF010000000
VERSION     DAZGHF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.033"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            150..1736
                     /gene="pdeC"
                     /locus_tag="ACHK3A_001784"
     CDS             150..1736
                     /gene="pdeC"
                     /locus_tag="ACHK3A_001784"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO8739871.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGVYLATHVDRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(1739..2062)
                     /gene="soxS"
                     /locus_tag="ACHK3A_001785"
     CDS             complement(1739..2062)
                     /gene="soxS"
                     /locus_tag="ACHK3A_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO8739872.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            2148..2612
                     /gene="soxR"
                     /locus_tag="ACHK3A_001786"
     CDS             2148..2612
                     /gene="soxR"
                     /locus_tag="ACHK3A_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO8739873.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            3158..4507
                     /gene="ghxP"
                     /locus_tag="ACHK3A_001787"
                     /gene_synonym="yjcD"
     CDS             3158..4507
                     /gene="ghxP"
                     /locus_tag="ACHK3A_001787"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO8739874.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4658..6307
                     /gene="yjcE"
                     /locus_tag="ACHK3A_001788"
     CDS             4658..6307
                     /gene="yjcE"
                     /locus_tag="ACHK3A_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO8739875.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6390..8039)
                     /gene="actP"
                     /locus_tag="ACHK3A_001789"
     CDS             complement(6390..8039)
                     /gene="actP"
                     /locus_tag="ACHK3A_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO8739876.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(8036..8350)
                     /gene="yjcH"
                     /locus_tag="ACHK3A_001790"
     CDS             complement(8036..8350)
                     /gene="yjcH"
                     /locus_tag="ACHK3A_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO8739877.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(8732..10690)
                     /gene="acs"
                     /locus_tag="ACHK3A_001791"
     CDS             complement(8732..10690)
                     /gene="acs"
                     /locus_tag="ACHK3A_001791"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO8739878.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            11083..12519
                     /gene="nrfA"
                     /locus_tag="ACHK3A_001792"
     CDS             11083..12519
                     /gene="nrfA"
                     /locus_tag="ACHK3A_001792"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO8739879.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            12564..13130
                     /gene="nrfB"
                     /locus_tag="ACHK3A_001793"
     CDS             12564..13130
                     /gene="nrfB"
                     /locus_tag="ACHK3A_001793"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO8739880.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            13127..13798
                     /gene="nrfC"
                     /locus_tag="ACHK3A_001794"
     CDS             13127..13798
                     /gene="nrfC"
                     /locus_tag="ACHK3A_001794"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO8739881.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            13795..14751
                     /gene="nrfD"
                     /locus_tag="ACHK3A_001795"
     CDS             13795..14751
                     /gene="nrfD"
                     /locus_tag="ACHK3A_001795"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO8739882.1"
                     /translation="MMQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTKPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            14771..16489
                     /gene="nrfE"
                     /locus_tag="ACHK3A_001796"
     CDS             14771..16489
                     /gene="nrfE"
                     /locus_tag="ACHK3A_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO8739883.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            16482..16865
                     /gene="nrfF"
                     /locus_tag="ACHK3A_001797"
     CDS             16482..16865
                     /gene="nrfF"
                     /locus_tag="ACHK3A_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO8739884.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEAEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            16862..17458
                     /gene="nrfG"
                     /locus_tag="ACHK3A_001798"
     CDS             16862..17458
                     /gene="nrfG"
                     /locus_tag="ACHK3A_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO8739885.1"
                     /translation="MKQSQIPVKMLTTLTILMAFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            17800..19113
                     /gene="gltP"
                     /locus_tag="ACHK3A_001799"
     CDS             17800..19113
                     /gene="gltP"
                     /locus_tag="ACHK3A_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO8739886.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(19531..20220)
                     /gene="yjcO"
                     /locus_tag="ACHK3A_001800"
     CDS             complement(19531..20220)
                     /gene="yjcO"
                     /locus_tag="ACHK3A_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO8739887.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20314..22461)
                     /gene="fdhF"
                     /locus_tag="ACHK3A_001801"
     CDS             complement(20314..22461)
                     /gene="fdhF"
                     /locus_tag="ACHK3A_001801"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22042..22044),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO8739888.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNKTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDTDQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(22659..24125)
                     /gene="mdtP"
                     /locus_tag="ACHK3A_001802"
     CDS             complement(22659..24125)
                     /gene="mdtP"
                     /locus_tag="ACHK3A_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO8739889.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPFYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTLRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQASPVVEKK"
     gene            complement(24122..26173)
                     /gene="mdtO"
                     /locus_tag="ACHK3A_001803"
     CDS             complement(24122..26173)
                     /gene="mdtO"
                     /locus_tag="ACHK3A_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO8739890.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            complement(26173..27204)
                     /gene="mdtN"
                     /locus_tag="ACHK3A_001804"
     CDS             complement(26173..27204)
                     /gene="mdtN"
                     /locus_tag="ACHK3A_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO8739891.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGIDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(27223..27498)
                     /gene="ytcA"
                     /locus_tag="ACHK3A_001805"
     CDS             complement(27223..27498)
                     /gene="ytcA"
                     /locus_tag="ACHK3A_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO8739892.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(27707..29692)
                     /gene="yjcS"
                     /locus_tag="ACHK3A_001806"
     CDS             complement(27707..29692)
                     /gene="yjcS"
                     /locus_tag="ACHK3A_001806"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO8739893.1"
                     /translation="MNNSRLFRLSRIVIALTAASSMMVNTANATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETI
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAKEANKQGDYRWSAELLKQVIAANPGDQVAKNLQADNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPEANASFYISREDLHAVLTGQVKMV
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(29965..30894)
                     /gene="alsK"
                     /locus_tag="ACHK3A_001807"
     CDS             complement(29965..30894)
                     /gene="alsK"
                     /locus_tag="ACHK3A_001807"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO8739894.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            complement(30878..31573)
                     /gene="alsE"
                     /locus_tag="ACHK3A_001808"
     CDS             complement(30878..31573)
                     /gene="alsE"
                     /locus_tag="ACHK3A_001808"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO8739895.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDNHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            complement(31584..32564)
                     /gene="alsC"
                     /locus_tag="ACHK3A_001809"
     CDS             complement(31584..32564)
                     /gene="alsC"
                     /locus_tag="ACHK3A_001809"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO8739896.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(32543..34075)
                     /gene="alsA"
                     /locus_tag="ACHK3A_001810"
     CDS             complement(32543..34075)
                     /gene="alsA"
                     /locus_tag="ACHK3A_001810"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFO8739897.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAEAQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCHPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     HE"
     gene            complement(34203..35138)
                     /gene="alsB"
                     /locus_tag="ACHK3A_001811"
     CDS             complement(34203..35138)
                     /gene="alsB"
                     /locus_tag="ACHK3A_001811"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO8739898.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGETRRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(35197..36087)
                     /gene="alsR"
                     /locus_tag="ACHK3A_001812"
     CDS             complement(35197..36087)
                     /gene="alsR"
                     /locus_tag="ACHK3A_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO8739899.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
BASE COUNT         8254 a         9317 c         9602 g         9001 t
ORIGIN      
        1 attattttta tccaggtgac cattaaaaag gtatcaattt caaatcaggc aaaagtgcta
       61 tttataccgt aagatttatc taaagacgtc ggtgcccagg gttttcacct tgcaatggcc
      121 gggtataaac aggcaggaaa ttgatagcaa tgagtcatcg tgcacgacac caattactgg
      181 cgttgccggg cattatcttt ttagttctct ttcccatcat tctgtcgcta tggattgcct
      241 tcctttgggc aaaatcagaa gtgaatagtc agctccaaac ttttgctcag ctggcgctgg
      301 ataaatccga gctggtcatt cgccaggcag atttagtgag cgatgcagca gaacgctatc
      361 aggggcaagt ttgcactcca gcccatcaaa agcgaatgtt gaatattatt cgtggctatc
      421 tttatattaa tgaattgatc tatgcccgtg ataaccattt tttatgctca tcgctgatag
      481 cgtctgtaaa tggctatacg attgcaccgg ccgattataa gcgtgaacct aacgtttcta
      541 tctattatta ccgtgatacg ccttttttct ctggctataa aatgacctac atgcagcggg
      601 gaaattatgt ggcggttatc aaccctctct tctggagtga agtgatgtct gatgatccga
      661 cattgcaatg gggagtgtat gatacggtga cgaaaacctt tttctcgtta agcaacgagg
      721 cctcggcagc aacgttttct ccgctgattc atttgaatga tttaaccgta caacgaaatg
      781 gctatttata tgcgacagtt tattcgacaa aacgcccaat tgcagccatt gttgcgactt
      841 catatcaacg tcttataact catttttata atcatcttat ttttgcgttg cccgccggta
      901 ttttggggag tcttgttctg ctattactct ggctacgtat tcgacaaaac tatttgtctc
      961 ccaaacgcaa attgcaacgc gccctcgaaa aacatcaact ttgcctttat taccagccaa
     1021 taatcgatat caaaacagaa aaatgtatcg gagctgaagc attgttacgt tggcctggtg
     1081 agcaggggca agtaatgaat ccggcagagt ttattccgct ggcagaaaag gaggggatga
     1141 tcgaacagat aactgattat gttattgata atgtcttccg cgatctgggc gtgtacctgg
     1201 caacacatgt cgatcgctat gtttctatta acctgtcggc ctccgatttt catacgtcac
     1261 ggttgatagc gcgaaccaac cagaaaacag agcaatatgc ggtgcgtccg cagcaaatta
     1321 aatttgaagt gactgaacat gcatttcttg atgtcgacaa aatgacgccg attattctgg
     1381 ctttccgcca ggctggttac gaagtggcaa ttgatgattt tggtattggc tactctaact
     1441 tgcataacct taaatcattg aatgtcgata ttttgaaaat cgacaaatcg tttgttgaga
     1501 cgctgaccac ccataaaacc agtcatttga ttgcggaaca tatcatcgag ctggcgcaca
     1561 gtctggggtt aaaaacgatc gctgaaggcg tcgaaactga ggagcaggtt aactggctgc
     1621 gcaaacgcgg cgtgcgctat tgtcagggat ggttctttgc gaaggcgatg ccgccgcagg
     1681 tgtttatgca atggatggag caattacccg cgcgggagtt aacgcgcggg caataaaact
     1741 acaggcggtg gcgataatcg ctgggagtgc gatcaaactg ccgacggaaa acgcgggaga
     1801 aggtctgctg agagacataa cccaggtcca ttgcgatatc aaaaatcgga cgctcggtgg
     1861 tgcgcaactc aacggcggcc agtaacaggc ggcgttggcg aatgtaatca cctagtgtct
     1921 gatgcgtcac agtacggaac atccgctgta agtaccactt tgaatagcct gatttttttg
     1981 cgacgacatc aatgttaagc ggctggtcaa tatgctcgtc aatccatgcg ataagatcct
     2041 gaataatttt ctgatgggac ataaatctgc ctcttttcag tgttcagttc gttaattcat
     2101 ctgttgggga gtataattcc tcaagttaac ttgaggtaaa gcgatttatg gaaaagaaat
     2161 taccccgcat taaagcgctg ctaacccccg gcgaagtggc aaaacgcagc ggtgtggcgg
     2221 tatcggcgct gcatttctat gaaagcaaag ggttgattag cagtatccgt aatagtggca
     2281 atcagcggcg atataaacgt gatgtgttgc gatatgtcgc aatcatcaaa attgctcagc
     2341 gtattggcat tccgctggcg accattcgtg aagcatttgg cgtgttgccc gaagggcata
     2401 cattaagtgc gaaagagtgg aaacagcttt cgtcccaatg gcgagaagag ttggatcggc
     2461 gcattcatac cttagtggcg ctgcgtgacg agctggacgg atgtattggt tgtggctgcc
     2521 tttcgcgcag tgattgccca ttgcgtaacc cgggcgaccg cttaggagaa gaaggcacag
     2581 gcgcacgctt gctggaagat gaacaaaact aaagcgccac aagggcgctt tagtttgttt
     2641 tccggtcttt gtctttctct ctatcccgct ggtacacagg agggtttccc ccgacgtcaa
     2701 cacacctcat tcgagcacgt ggtggaggtt ccggttggtg ttgatgcttt aattgtatgt
     2761 caccggcgtt tcttcgccag tgtaaaagta cactttttag ccgcaatatt tttgtcatct
     2821 cagacgattt tttatcgcaa tcctgaacgg tatacggctc gataacgctg caatcttgcg
     2881 catcgacgat aacgtttgcg catcaattgc ctggtttttc atcgtcaaga caataaaaga
     2941 gaaaaaagca gcaaacttcg gttgaaaaag ccgctatgat cgccggataa tcgtttgctt
     3001 tttttaccac ccgttttgta tgcgcggagc taaacgtttg cttttttgcg acgcaccaat
     3061 ttgtcgcaaa cctggagcag gaagataacg tttcgctggc aggggattgt ccgccacgca
     3121 tcttgacgaa aattaaactc tcaggggatg ttttcttatg tctacgccat cagcgcgtac
     3181 cggcggttca ctcgacgcct ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga
     3241 agtggttgcc gggttaacaa catttctggc gatggtctac tcggttatcg tcgttccagg
     3301 catgttgggt aaagcaggct tcccgcctgc ggcagttttc gttgcgacct gtctggttgc
     3361 cggactcggt tctatcgtga tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc
     3421 catctccctg acggcgttta ccgcattcag tctggtgctg gggcaacata ttagcgtacc
     3481 tgtcgcgctg ggtgccgtgt tcctgatggg tgtgctgttt acggtcattt ctgccacggg
     3541 tatccgtagc tggattttgc gcaacttgcc gcacggtgtg gcgcacggca cggggattgg
     3601 tatcggtctg ttcctgctgc tgattgctgc taatggtgtc ggtctggtga ttaaaaaccc
     3661 acttgatggt ctgcccgtcg cgctggggga tttcacgacc tttccggtga tgatgtcgtt
     3721 ggtggggctg gcagtgatta tcggcctgga aaaactgaaa gtccctggtg gcattctgct
     3781 gaccattatc ggtatctcaa ttgtcggttt gatcttcgat cctaacgtcc atttctccgg
     3841 cgttttcgcc atgccttcat tgagcgatga aaacggcaat tcactaattg gcagcctgga
     3901 cattatgggc gcgctgaatc ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc
     3961 agtatttgat gccaccggaa ctatccgtgc cgtcgccggc caggcgaacc tgctggataa
     4021 agatgggcag atcatcgacg gcgggaaagc actgaccact gactccatga gcagcgtttt
     4081 ctctggcctg gtgggtgcag ctccggcagc ggtatacatc gagtctgcgg cgggtacggc
     4141 ggcgggcggt aaaaccggtt tgacggctat caccgttggc gtgctgttcc tgctgattct
     4201 gttcctctct ccgctctctt acctcgttcc ggggtatgca acggctccgg cgctgatgta
     4261 cgttggcctg ctgatgctga gtaacgtggc gaaaatcgac tttgctgatt ttgttgatgc
     4321 gatggcgggt ctggttacgg cggtattcat cgtgctgacc tgtaacatcg taacaggcat
     4381 catgatcggc ttcgcgactc tggtgattgg tcgtctggtt tccggcgaat ggcgcaagtt
     4441 gaacatcggt acggtcgtta tcgctgtggc gctggtgacc ttctatgcgg gtggctgggc
     4501 tatctaatct ttcatatgat tctgaaaacg ggtggcaatg gctgcccgtt tttattttct
     4561 ccgcacattg tgtgagcttt tgcgatattc tgaaaaaatg agaattcagg cataacgcct
     4621 ttctcagatc ataaaaaaca tcgcaaacag ggaacgcatg gaaatcttct tcaccatact
     4681 gataatgacc ctcgtggtct cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat
     4741 cccgcttccg cttatgcaaa tcgccatcgg tgcgctactg gcgtggccga cgtttggttt
     4801 gcatgtggag tttgatcctg aactcttttt agtcttgttt atcccgccgt tgctgttcgc
     4861 tgatggctgg aaaacgccga cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct
     4921 cgcgctggcg ctggtggtgg tcaccgtggt cggcattggc tttcttattt actgggtggt
     4981 gccgggcatt ccgctgatcc ccgcctttgc gctggcggcg gtgctttctc cgaccgatgc
     5041 tgtggcgctc tcggggattg ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt
     5101 gcagggcgaa gcgttgatga atgacgcctc cggtctggtg tcgttgaagt ttgccgtggc
     5161 agtggcgatg gggacgatga tcttcaccgt cggtggtgcg acggtcgaat ttatgaaagt
     5221 ggccattggc ggtattctcg ccggttttgt ggtgagctgg ctgtacggtc gctcgctgcg
     5281 attcctcagc cgctggggcg gtgatgaacc cgcgacgcag atcgtcctgc tgttcctgct
     5341 gccattcgct tcttatctga ttgccgaaca tattggcgtt tccggtatcc tcgctgcggt
     5401 tgccgccggg atgaccatca cccgttccgg tgtgatgcgc cgtgcgccgc tggcaatgcg
     5461 cctgcgcgca aacagtacct gggcgatgct ggaatttgtc tttaacggca tggtattcct
     5521 gctgttaggt ctgcagctgc cgggtattct ggagacgtcg ctgatggcgg cagaaatcga
     5581 ccctaacgtc gaaatctgga tgctgtttac cgatatcatt ctgatttatg cggcgctgat
     5641 gctggtccgt ttcggctggc tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa
     5701 gaagccgatg gagtttggtt cgtggaccac acgagaaatc ctgatcgcgt ctttcgccgg
     5761 ggtgcgtggg gcgatcactc tggctggtgt gctctcaatc ccgctgctct tgccggatgg
     5821 taacgtcttc ccggcgcgct atgagctggt gttcctggcg gctggtgtca ttctcttctc
     5881 gctgtttgtc ggcgtggtga tgttgcctat tctgctacaa cacattgaag tcgcggacca
     5941 ttcgcaacaa ttgaaagagg aacgtattgc gcgagcggca acggcagaag tggcgattgt
     6001 ggcgatccag aaaatggagg agcgtctggc ggcggatacc gaagagaata tcgataacca
     6061 gctgctcaca gaggttagtt ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg
     6121 taacgacgtt gaaagttccg tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc
     6181 attgcgttct gaacgtgctg aactgtacca cctgcgcgcc acgcgggaga tcagtaacga
     6241 aacgctgcaa aaattactgc acgatctcga tttgcttgaa gcgttgctga ttgaagagaa
     6301 ccaataacgc tttggcggtg taatcggtaa aaaggtgcag tacatcgcac ctttttaccg
     6361 ttggtcaggt gaccagaggc tagtggagat taatgcgcgc ggccttgctc aacgccaaag
     6421 ccagtctggg agcggataaa ctgcgcacgg aacagttcac gctcgcgcgc accttccgct
     6481 gaattatccg ttgccgagaa gaaccagata ccgaggaatg ccacgctgat agagaacagt
     6541 gccgggtatt catacgggaa gatggctttt tcgtgaccga ggatctgtac ccaaatcgtc
     6601 ggaccgagga tcatcagcac caccgcagta atcagtccta accagccgcc cagcattgcg
     6661 ccacgggtag tcagtttcga ccagtacatg gaaagcagaa tgatcgggaa gttacagctc
     6721 gccgcgatgg caaacgccag ccccaccata aaggcgatgt tctgattctc aaacagcacg
     6781 ccgaggataa tggcaatcac acccaaaatc agaacggtga ttttcgatac ccgcagctct
     6841 tcacgttcgg tcgcgccttt tttgaagacg ttagcgtaca ggtcgtgaga caccgccgat
     6901 gcgcccgcca gcgtcagccc cgctaccacc gcgaggatag tggcgaaagc aaccgctgaa
     6961 ataaagccga ggaacaggtt gccgcccacc gcattggcca ggtgaactgc cgccatgttg
     7021 ttaccaccaa tcagatggcc cgcagcatct ttatattccg gattcgcacc aaccagcatg
     7081 attgcgccga agccgataat aaaggtcagg atgtagaagt agcccataaa tccggtggcg
     7141 tagaacacgc tcttacgtgc ttcgcgggca tcgctgaccg taaagaagcg catcagaata
     7201 tgcggcaagc ccgccgtacc aaacatcagt ccgagcccca gcgacagtgc tgagatcgga
     7261 tctttcacca gcccgcctgg cttcatgata tcgacacctt tcgggtgtac cgccatcgct
     7321 tcgctgaaca gattgttgaa gctaaagccg acgtgtttca tcaccataaa ggccataaag
     7381 ctggcaccga acagcaacag aacggctttg ataatctgta cccaggtggt cgccagcatg
     7441 ccgccgaaca ggacgtacat catcatcagc acgccgacca gcaccaccgc aatgtgatag
     7501 ttaaggccaa acagcagctc gatgagttta cctgcgccca ccatctgggc gataaggtaa
     7561 agcgccacca ccaccagaga accacaggcc gaaagaatac gaatcggccc ttgtttcagg
     7621 cggtaagagg ctacatcggc aaaggtgtag cggcccaggt tacgcagacg ttcggcaatc
     7681 aggaacagaa tgattggcca gcctaccagg aagcccagcg agtaaattaa gccgtcatag
     7741 ccggaggtaa acaccagcgc ggagatcccc aagaatgagg cggcggacat atagtccccg
     7801 gcaatcgcca gcccattctg aaaaccggtg atattgccgc ctgcggtgta gtagtcatta
     7861 cgcgaacgta cgcgttttga cgcccagtag gtaatgccga gcgtaaacac gacgaaaatc
     7921 aggaacataa taatcgcctg ccagtttgtt ggctggcgct ctacggcccc gctaatagca
     7981 tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg ccgtcagaac tcttttcatg
     8041 atgcttgtac ctcatgcagg acttcattat taagacggtc gaattcgccg ttcgcccgcc
     8101 agatgtagat accagtgaga acaaaggaga tcacaattac tccaacacca atcggaatac
     8161 cgcgtgtgac gctggtgttc ggattcagcg gggtgcccag ccagccgggc gcgaaggcga
     8221 tcagtaaaat aaagccgata taaactgcca gcataataat cgacaggatg gtggcaaacc
     8281 gttgccgttt ttcgactaac tccctgaaat gcgcattgtc ttctatccgc tgataaatcg
     8341 taccattcat cacagattct ccagaggtaa tgtagggatt gttttaattc ccgctccctt
     8401 atgggagaag gttaacgctt gggtaaccct tgccgaatgt aggccggata aggcattcac
     8461 gccgcatccg gcaatcaacg cctgatgcga cgctgtcgcg tcttatcagg cctacagccg
     8521 ttgccgaatg taggccggat aaggcgttta cgccgcatcc ggcaatcaac gcctgatgcg
     8581 tcgctgtcgc gtcttatcag gcctacagcc gttgccgaat gtaggccgga taaggcgttc
     8641 acgccgcatc cggcaaccag cgcctgatgc gacgctgtcg cgtcttatca ggcctacaaa
     8701 ccgttaccga ctcgcatcgg gcaattgtgg gttacgatgg catcgcgata gcctgcttct
     8761 cttcaagcag cttctcgact acgccaggat cggcaagcgt cgaggtatcg cccaggttgc
     8821 tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg gagcgggttt
     8881 taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca atctctttac
     8941 gcacccagtt gcggacttct gcgtacagtt ctggtgacgg ttcctcccca tgattaagcg
     9001 tgacgtaggc gtagatcgcc tgacctttaa tattgtgcgg aataccgacg acagcggctt
     9061 cggcgatttt cggatgcgcc accagcgccg actcaatctc cgccgttccc agacggtgac
     9121 ccgagacgtt cagcacgtcg tccacgcgcc cggtgatcca gtaatagcca tcttcatcgc
     9181 gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt ggagaagtag gtctgctcaa
     9241 aacgttcgtg atcgccaaac agcgtacgcg cctgacccgg ccaggagtcg gtgatcacca
     9301 gactgccttc ggtggcccct tccagcgggt taccttcgtt atcgaccagc gccggttgca
     9361 cgccgaagaa cggacgtgtt gccgaaccgg ctttcagctc ggtagcgcca ggcagcgggg
     9421 tgatcatgaa accgccggtt tcggtctgcc accaggtatc gaccaccggg catttctcgt
     9481 tgccgatttt tttccagtac cactcccacg cttccgggtt gattggctcg cctacggaac
     9541 cgagaatgcg cagcgacgaa cggtcagtgc cttcgatcgc tttatcgcct tccgccatca
     9601 gcgcgcggat cgccgtgggc gcggtataga gaatattgac ctgatgcttg tccaccacct
     9661 gcgccatacg ggcaggcgtc ggccagttcg gtacgccttc aaacatcagc gtggtcgcgc
     9721 cgcaggccag cgggccgtac agcaaataac tgtgtccggt aacccagccc acatcggcgg
     9781 tgcaccagta gatatcaccc ggatgataat caaagacata tttaaaggtc agcgccgcgt
     9841 acaccagata accgccggta gtgtgcagca cgcctttcgg cttcccggta gaaccggagg
     9901 tgtagagaat aaacagcgga tcttcggcgt tcatctcttc cgcctggtgc tgatcgctgg
     9961 cttgctcaac caggtcgtgc caccacaggt cgcgcccttc ctgccagtca attttcccgc
    10021 cagtacgctt cagtaccacc acatgttcaa tgctggtgac gttcgggttt ttcagcgcgt
    10081 catcaacgtt tttcttcagc ggaatactac gcccggcacg cacgccttcg tcggaagtga
    10141 tcaccagtcg tgagttagaa tcgataatgc gtccggcaac ggcttccggc gagaagccgc
    10201 cgaaaattac cgaatgcacc gcgccaatgc gggcgcaggc cagcatcgcg accgcggctt
    10261 ccggcaccat cggcatataa atcgccacca catcaccttt tttaatgccc agctcgagca
    10321 gggtattggc gaagcggcag acgtcgcggt gcagctcttt atagctgata tgtttgctct
    10381 ggctggcgtc gtcgccttcc cagatgatgg cggtacgatc gccgttttct tgcagatggc
    10441 ggtcaaggca gtttgccgcc agattcagag tgccgtcctc gtaccattta atggacacat
    10501 taccgggggc aaaggaggtg tttttcacct tctggtaagg tttgatccaa tcgagaattt
    10561 ttccctgttc gccccagaag gtatcaggtg cgttaataga ttgttgatac atcgcctcgt
    10621 actgctgagg gtttatcagg caacggtctg cgatgttggc aggaatggtg tgtttgtgaa
    10681 tttgactcat gcttttgttc tccttgtagg atgttaataa tatgtggcat aagcgttaaa
    10741 tgtaggggta ttggcagttt tgtttagtat ttgggcgaca gatcacgcaa aagtagaatt
    10801 gtgcaaataa acggcagggt aatttttgaa ggtcagcaac aaaagttgat taattctttg
    10861 aggaacatgc agttatgcat gctgttgaaa aagaggaaga tactgactaa ctctaaagtg
    10921 gtattttaca tgcacttaca attgattaaa gacaacattc acagtgtggt tatttgttac
    10981 acataggggc gagcaatgtc atgacagtgt aggtgcggtt actgtcgtga aaaacaataa
    11041 aaaccgccat tgcaacaatg gcgcaattcg gatgaagccc ctatgacaag gataaaaata
    11101 aacgcacgcc gtatcttcag cttattgatt ccttttttct ttttcacttc tgttcacgct
    11161 gaacaaacgg cagctcctgc aaaacctgta actgttgaag cgaagaatga aacctttgcc
    11221 ccgcaacatc ccgatcaata tctctcctgg aaagccacct cggaacagtc agagcgtgtt
    11281 gacgccctgg cggaagatcc acggctggtg atcctgtggg cggggtatcc cttctcgcgc
    11341 gattacaaca agccgcgtgg acatgccttt gctgtgaccg atgtgcgtga aaccctgcgt
    11401 accggtgcgc cgaaaaacgc tgaagatggt ccgctaccga tggcatgctg gagttgtaaa
    11461 agcccagatg tggcgcgtct gatccagaaa gacggcgaag atggctactt ccacggaaaa
    11521 tgggcgcgcg gcggcccgga aatcgtcaac aacttaggtt gtgccgattg ccataacacc
    11581 gcctcaccag agttcgccaa aggcaaaccg gagttaaccc tttcccgtcc gtatgcggct
    11641 cgcgcgatgg aagccattgg taaacctttt gagaaagccg gacgtttcga ccagcaatcg
    11701 atggtttgcg gtcagtgcca tgtggagtat tacttcgacg gcaaaaacaa agcggttaaa
    11761 ttcccgtggg atgacggcat gaaagtcgaa aatatggagc agtattacga caaaattgcc
    11821 ttctctgact ggactaactc cctgtcgaaa acgccaatgc tgaaagcgca gcacccggaa
    11881 tatgaaacct ggacagcggg cattcacggc aaaaacaacg tgacctgtat cgattgccat
    11941 atgccaaaag tgcagaacgc cgaaggcaaa ctctacaccg accataaaat tggtaatccg
    12001 tttgataact tcgcccagac ttgtgcgaac tgccataccc aggacaaagc tgccttgcaa
    12061 aaagtggtcg cggaacgtaa gcagtcgatt aacgacctga aaatcaaggt tgaagatcaa
    12121 ctggttcacg ctcacttcga agcgaaagcg gcgctggatg caggcgcaac ggaagccgaa
    12181 atgaagccga ttcaggacga tatccgtcat gcccagtggc gttgggattt ggcgatcgct
    12241 tcccacggca ttcatatgca cgcaccggaa gaaggtctga ggatgctcgg tacggcgatg
    12301 gataaagcgg cggatgcacg caccaaactg gcgcgcctgc tggcgaccaa aggcattacc
    12361 catgaaatcc agatcccgga tatctcgacc aaagagaaag cccagcaggc cattggcctg
    12421 aacatggagc aaatcaaggc cgagaagcag gacttcatca aaacggtgat cccgcagtgg
    12481 gaagagcagg cacgtaaaaa cggtctgtta agccaataac cccgttccgc ctcgcaaggg
    12541 gtggaaaaca caatggagtg aatatgagcg tattacgttc gttattaact gccggggtgc
    12601 tggcgtcggg cctgttgtgg agcctgaacg gaattaccgc tacccctgcg gcgcaggcat
    12661 ctggtgatcg ctacgaagtt acccagcagc gtaacccgga tgccgcctgt ctggactgtc
    12721 ataagccaga taccgaaggt atgcatggca aacatgcttc cgttatcaac ccgaataaca
    12781 aactgccggt cacctgcacc aactgccacg gtcagccatc accgcaacac cgcgaagggg
    12841 tgaaagatgt gatgcgcttt aacgagccga tgtacaaggt tggagagcag aacagcgtct
    12901 gtatgtcctg ccatctgccg gaacagttgc aaaaagcgtt ctggccacac gatgtccacg
    12961 taaccaaagt ggcgtgcgcc agctgccatt ccctgcatcc acagcaagat acgatgcaga
    13021 cgttaagtga caaaggacgg attaagattt gcgtcgattg ccacagcgat cagcgcacca
    13081 atccgaactt taacccggcg tctgttccgt tgcttaagga gcagccatga cctggtctcg
    13141 tcgccagttt ctcaccggcg tcggcgtgct ggcagccgtc agcggcaccg cagggcgcgt
    13201 ggtggcgaaa acgttgaata tcaatggggt gcgttacggc atggtgcatg acgagtcgtt
    13261 atgcatcggc tgtaccgcct gtatggatgc ttgtcgggaa gtgaacaagg tgccggaagg
    13321 cgtctcgcgc ctgacgatta ttcgtagcga gccgcagggc gaatttcctg atgtgaaata
    13381 tcgcttcttc cgtaagtctt gccagcactg cgatcatgcg ccgtgcgttg acgtctgccc
    13441 gaccggtgcg tcttttcgtg atgccgctag cgggattgtc gatgttaatc ctgatctctg
    13501 cgtcggttgt cagtactgca tcgccgcctg tccgtaccgc gtgcgcttta tccatccggt
    13561 cacgaagacg gcggacaaat gcgatttctg ccgtaagacc aatttgcagg ccggtaagct
    13621 gcccgcgtgc gttgaagctt gcccgaccaa ggcactgacg tttggcaatc tggacgatcc
    13681 caacagtgag atttcgcaac tgctgcgcca gaagcccact taccgctaca agctggcgct
    13741 gggaaccaaa ccgaagctgt accgcgtacc gtttaaatac ggggaggtga gccaatgatg
    13801 cagacttccg catttcattt tgaatcgctg gtgtgggact ggccgattgc catctacctg
    13861 tttttgattg gtatttctgc cggtctggtg acgctggccg tgctgttacg tcgcttctac
    13921 ccgcaggcgg gcggtgcaga cagtacgttg ctgcgcacca cgctaattgt cgggcctggc
    13981 gcggtgatcc tcggtctgtt gatcctcgtt ttccacctga caaagccgtg gaccttctgg
    14041 aagctgatgt tccactacag ttttacctcg gtgatgtcga tgggggtgat gttgtttcag
    14101 ctctacatgg tggtgctggt gctgtggctg gcgaaaatct ttgaacatga tttgcttgcc
    14161 ctgcaacaac gctggttgcc gaagctgggg atcgtgcaaa aggttctgag cctgctgacg
    14221 cccgttcatc gcggactgga aacattgatg ctggtgctgg cggtgctgct gggggcttat
    14281 accggctttc tgctgtcggc gctgaaatcg tatccgttcc tcaataaccc gatcctgccg
    14341 gtgctgttcc tcttctccgg catctcgtcc ggtgcggcgg tagcgctgat cgccatggcg
    14401 atacgccaac gcaaaaatcc gcattccacg gaagcgcact ttgttcaccg tatggaaatc
    14461 cctgtggtat ggggtgaaat cttcctgctg gtggcgtttt ttgtcggtct ggcgctgggc
    14521 gatgacggta aagtgcgtgc gctggtggcg gcattaggtg gcggtttctg gacgtggtgg
    14581 ttctggcttg gtgtcgccgg gctggggctg attgtgccaa tgttgctcaa accgtgggtc
    14641 aatcgcagtt ccggcattcc taccgtgctg gcggcgtgtg gggccagtct ggtcggcgtg
    14701 ttgatgctgc gctttttcat tctctacgcc ggacagttga cggtggcgta agccagaaaa
    14761 gaggtggttt ctggacgtat tccttcctga agtcggtttt ctggcgttgt tgttaagtct
    14821 cggggtcaac gtgttgaccc cgttgacggc cttcgcggga gtgcggttgc gctggcctgc
    14881 catgatgcga ctcacttgca tcggcattct ggcgcagttc gcgctcctgc tgctcgcctt
    14941 tggcgtactg acgtattgtt ttctcatcag cgatttctcg gtcatttatg tcgcccaaca
    15001 tagctacagc ctgctgtcgt gggaactcaa actggcggcg gtgtggggcg gtcatgaagg
    15061 ttcgctgctg ctttgggtgc tgctgctttc cgcctggagc gcgctgtttg cctggcatta
    15121 tcggcagcaa accgatccgc tatttccgct aacgctagcc gttttatctc tcatgctcgc
    15181 cgcactgcta ctgtttgtag tgctgtggtc cgatcccttc gtgcggatat ttccaccagc
    15241 aatcgaaggc cgcgatctca atccgatgct gcaacatccc ggtcttatct ttcatccacc
    15301 gctgctttac cttggctatg gcggtttgat ggtggcggcg agcgtggcgc tggcgagttt
    15361 actgcgcggc gagtttgatg gtgcctgcgc ccgaatttgc tggcgctggg cgttacctgg
    15421 ctggagtgca ttaacggctg ggatcatcct cggttcctgg tgggcctatt gcgaactggg
    15481 ctggggcggc tggtggttct gggatccggt ggaaaacgcc tctttattac cctggctttc
    15541 tgccactgcg ctgctgcaca gtttgtccct gacacgccag cgggggattt tccgccactg
    15601 gtcgctgctg ctggcgatag ttactctgat gctgtcgctg ctgggcacct taattgtccg
    15661 ttctggcatt ctggtttcgg ttcatgcgtt cgcactggat aacgtccgcg ccgtgccgtt
    15721 gttcagcctg tttgcactga ttagccttgc gtctctggct ctgtatggct ggcgagcgcg
    15781 ggacggtggc ccggcggtgc gtttttcggg gttatcgcgg gaaatgttaa tcctcgctac
    15841 gctgttgctg ttttgcgcag tgctactgat cgtgctggtg ggaacgcttt atccgatgat
    15901 ttacggtctg ctgggctggg gacgcctctc cgttggcgcg ccgtatttta accgcgcgac
    15961 gttaccgttt ggtctgttga tgctggtggt gattgtgctg gcgacgtttg tctctggcaa
    16021 acgcgtgcag cttccggcgc tggtagctca tgctggcgtg ctgttatttg ccgcggggat
    16081 cgtggtttcc agcgtcagtc gtcaggagat cagcctgaat ttacagccgg gtcagcaggt
    16141 gacgctggca ggatacacct tccgttttga gcgactcgat ctgcaagcca gaggcaatta
    16201 caccagcgaa aaagcgatag tggcgctgtt tgaccatcag caacgcattg gtgaactgac
    16261 gccggagcgg cgtttttatg aagcacgccg tcagcaaatg atggaaccgt caattcgctg
    16321 gaacggcatc catgactggt atgcggtcat gggcgagaaa accggagcgg atcgttacgc
    16381 ttttcgtttg tatgtacaaa gcggtgtgcg ctggatctgg gggggaggat tgttgatgat
    16441 tgcgggcgca ttgttaagcg gatggcgggg gaggaagcgc gatgaataaa ggacttctca
    16501 cgctactgct gttatttacc tgttttgcgc gcgctcaggt cgtagacacc tggcaattcg
    16561 ccaatccgca acaacagcaa caggcgttaa atattgccag ccagttacgt tgtccgcagt
    16621 gccagaatca aaacttactg gaatccaacg cgccggtggc ggtcagtatg cgccatcagg
    16681 tttacagcat ggtggcggag gggaagaacg aagctgaaat catcggctgg atgaccgaac
    16741 gctacggaga ttttgttcgc tataacccac cgttaacggg ccagacgctg gtgttgtggg
    16801 cgctgccagt ggtgttgtta ctgctgatgg cactgatcct ctggcgagtg agggcgaagc
    16861 gatgaaacag tcacaaatac cggtgaaaat gctgacaaca ctcacgattt tgatggcatt
    16921 tctctgtatc ggcagttatc tgttaagccc aaaatggcag gcggtacgtg cggagtatca
    16981 gcgtcagcgc gatccgctgc atcagtttgc cagccagcaa accccagaag cgcagcttca
    17041 ggcattgcag gataaaatcc gcgctaatcc acaaaacagc gaacagtggg cgttactggg
    17101 cgagtactat ctgtggcaaa atgattacag caattcgctg ctggcgtacc gtcaggcgtt
    17161 gcaactacgt ggtgagaacg ctgaactgta tgcggcgctg gcgacggtgc tttattacca
    17221 ggccagccag catatgaccg cccagacacg cgcaatgatc gataaagccc tcgcgctgga
    17281 ctctaacgaa atcaccgctc tgatgctgct ggcttccgat gcgtttatgc aggcgaatta
    17341 cgcgcaggcc atcgaactat ggcaaaaagt gatggatctc aactcacccc ggatcaaccg
    17401 gacacagctg gtcgagtcga ttaatatggc gaaattgttg cagcggagat cagattaatc
    17461 atctggtgat gaagattatg ttattgagct gcataaaaaa taatcgaatg aacatatgcc
    17521 aaaaataatc accaatcagt attattgcag attaaacaaa taaaaatctt tccataacaa
    17581 atggttattc attaatcctg ccataaactc gcgttattat gcattaatgc agcgaaaagc
    17641 tctgttgtta aagggttgcg caacataccg cgcaaatgat actgatcata agcgttaaaa
    17701 aaatctacaa accaacgcaa cacaattcat gccctggcag tatgtcacgt tctcgcgttt
    17761 ctgaacgggg aacggcgctc cattgaggaa gttattctga tgaaaaatat aaaattcagc
    17821 ctggcctggc agattctgtt tgctatggtg ctgggcattc tcctgggaag ctacctgcac
    17881 taccatagcg acagccgcga ctggctggtc gtcaatttgc tctctccggc gggtgatatc
    17941 ttcatccatc tgatcaaaat gattgttgtg ccgattgtga tctccacgct ggtggtgggt
    18001 atcgcgggtg ttggtgatgc caaacagctc gggcgtattg gcgcgaaaac cattatttac
    18061 ttcgaggtga tcaccaccgt cgccatcatt ttggggatca ctctggcgaa cgtcttccag
    18121 cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa atatcagagc
    18181 actacggaag cggtacaaag cagttcccac ggcattatgg gcacgatttt gtcgctggtg
    18241 ccgacgaaca ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat cttcttctcg
    18301 gtgctgtttg gtctggggct ttcatccctg cccgcgacgc atcgtgaacc gctggtgacc
    18361 gttttccgct ccatctctga aaccatgttt aaagtgactc acatggtgat gcgttatgcg
    18421 ccggtgggtg tgtttgcgct gattgcggtg acggtggcta actttggttt ctcgtctctg
    18481 tggccactgg cgaaactggt gctgctggtg catttcgcca ttctgttctt cgcgctggta
    18541 gtgctgggaa ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat tcgtattctg
    18601 aaagatgagc tgattctggc gtactccact gccagctctg aaagcgtgct gccgcgaatt
    18661 attgagaaga tggaagccta cggagcaccg gcgtcgatca ccagtttcgt ggtgccgacc
    18721 ggttactctt ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc tattttcatc
    18781 gcgcagttgt atggcattga cctgtccatc tggcaggaaa tcattctggt gctgacgctg
    18841 atggtgacct cgaaagggat tgctggcgtg ccgggcgtgt cgtttgtggt gttgctggca
    18901 acgctgggta gcgtaggtat tccgctggaa ggtctggcgt ttattgccgg tgttgaccgt
    18961 atcctcgaca tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc ggtgctggtg
    19021 attgccaagt gggaacacaa atttgaccgt aagaaagcgc tggcttatga gcgcgaagtg
    19081 ctgggcaaat ttgataaaac tgcggatcaa taattgaaga ttgccgggga tatccacccg
    19141 gcaatatgtg gatgcctgat gcgacgctta ccgcgtctta tcaggcctac gccagactgc
    19201 gcaatagctt gatttagggt gattttgtag gtcggataag gcgtttacgc cgcatccgac
    19261 atcaatatct gatgcgacgc ttgccgcgtc ttatcaggcc tacgccagac tgcgcaatag
    19321 cttgatttag ggtgattttg taggtcggat aaggcgttta cgccgcatcc gacatcaata
    19381 tctgatgcga cgcttaccgc gtcttatcag gcctacgcca gactgcgcaa tagcctgatt
    19441 tagcgtgatt ttgtaggtcg gataaggcgt ttacgccgca tccgacatca atgcctgatg
    19501 cgacgcttac cgcgtcttat caggcctacc ttaaccgtta gttaattttt caaactcttc
    19561 acacccggta tcaaaccctt ccatacagct cagatttaac cagtgcagcg ccttttgttt
    19621 gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc agtactcgga
    19681 ataaccggtt cgggaaatcg cagagctgcg tttgaaatac caggttgcct tgtcatcgtc
    19741 ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg catcgacggc
    19801 agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaattgcct ttggataatc
    19861 cggtttaccc gcttgcgtat ttaccagaat atgcgccagc gttacttcac cttctttact
    19921 gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca gactgaccgg
    19981 attggtgatt ttgatctgcg ctaacagcgc gcaggcatca gcatcaccgc tgtcggcggc
    20041 tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc tgtcggcaag
    20101 aaaatattgt gcgcgtcggt cccctgcctc tgctgccttt aaatactggc tgccaggctc
    20161 ggagtcgttc gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa tttttttcat
    20221 tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg ataaaaaaac
    20281 agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt cgcgcaggcg
    20341 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
    20401 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
    20461 ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
    20521 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
    20581 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
    20641 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
    20701 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt cggtgagttt
    20761 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
    20821 cttctcttta aacagataag aagtcccctg atcggtatct gaagtatcgc ggcaaggcca
    20881 ctgaatgaag cccagttcgc ccattttttc gtaagtcgca ccgtagaaat ccgggcacag
    20941 atgacgcaac tcatcccaga tctcctgggt tttgttgtag tgcatcggat aacccatacg
    21001 ggtggcgatt tcactgataa tctgccagtc cgttttcaga tcccacttcg gctcaaccgc
    21061 cttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
    21121 cgacgttgac ggtaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
    21181 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagtt ccgcgtcagt
    21241 ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcaccatgcg ctgcgcggtg
    21301 cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc cccaggcttt
    21361 ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat acgtatccgg
    21421 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
    21481 cgcatgaggc ttaccgaggt taccagtcag catcgcgagg ctggtcagag aacgcacggt
    21541 ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg cgcttttcgc
    21601 ctgggcatac atccgcgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
    21661 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
    21721 ggaagcgacg aaagctttgt cgtacaggtt ttcttcaata atgacatggc ccatcgcatt
    21781 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
    21841 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
    21901 gtggttcgcc acgattgggt gggaatccgc cgggttgtac ccgaaaacga acactaaatc
    21961 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
    22021 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
    22081 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cacgcgagga
    22141 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggc
    22201 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
    22261 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga aatcccagcc
    22321 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
    22381 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
    22441 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
    22501 tcgactacag tatgcatctt ttatgccaca ttttatttgt ggtcattccc tgatattacg
    22561 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
    22621 acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc gacgacggga
    22681 cttgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
    22741 ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc ttcggtggcc
    22801 tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgcag ggtaaagcgc
    22861 gttgcttcca cgcgttcagc ctgcatctct cgctcgtcgt tgagcgtttg cagacgcgtg
    22921 ccgttgacgg caacgtcgcg caccgcattc agtactgact ggttgtaacg ttcaatcatc
    22981 atgttgctgg cggcgcgagt gccttcgaga ttggcattca accgtccacc gtcaaacagc
    23041 ggcaatttca gacccgggat aaagttgaac tggcgactgg tttttttgaa taaggtatcc
    23101 aggtggatag agtccaggcc gaaaaacgct ttgatatcaa agctcggata gaacagcgcc
    23161 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatcgc ttgcagatcc
    23221 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg gacttgcggt
    23281 aatgccaccg gtttgatctc cggcatatcg ctggctcccg cgccaatcaa tgcacgcaga
    23341 gattctcgcg tttcggtgat ttgcccttta acggcaacaa tttgtttatc gaccgccaga
    23401 atctgtgccc gcgcgccgtg gaaaggcact tgcgcttcca gaccgtgcgc cactttactc
    23461 tggtgcgctt tcaccgcgta atcaatcaca tcgcgtgttt gttctaacag atcgagcatc
    23521 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
    23581 actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcagc aaccgctgag
    23641 cgatgcacac cccacaaatc gagatccagt ccggcaaaca aaccgactgt ggcttccgta
    23701 tagaacggcc cgtccatacc cagtgctggc gcatccatcg cataagggct taaaaagccg
    23761 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
    23821 gcatctaaca aatcggcctg cgactgcgct ttttcttccc gcagtttcgc ttcggcgagg
    23881 gtgtgtgaac cacttagcgt ccgttggatc agggcatcca actgcgggtc attgagttgt
    23941 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    24001 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tacgtaccag ggcacagccg
    24061 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca ggcgtgaaag ttgacgattg
    24121 atcattgcgt ggctccttgt acctgttccg tggcgggcgt taatgccggg gctgtccagt
    24181 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtcggcagga
    24241 tggcctgcga ggcgggtgtt tcttcaagcg ttatttgagg agaacgactg agtgcggttg
    24301 ccagaccggc ggcgtatttc tccagtgcgt cggcaaactg acctgctctg tccggctgta
    24361 gtgcgttatc cagcgcctgc gccgagttcc aggttgcatc ccaggcgtga agaatatcgc
    24421 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa agcgcgttct tcgctgtcca
    24481 gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    24541 gacctatgcg aatttgcaga taagtgcgca gagctgtgac ttcctgctgg cgtgggatcc
    24601 gcattacttt actcaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    24661 cgctttcagg ccagacaaag gtgtaaatca ccgccgacac cacggtacca atgatgatac
    24721 ccagagcgcg atcgcgaatt tccaccaggt cgtacaccgg gccaaaaacg ttttcgagcg
    24781 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    24841 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    24901 tgtccagcca gggcatgacc aataacgtga ataacagcgc cagaatcgcg ccgcaaaagg
    24961 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacattggga ttagcgacga
    25021 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg tagaaggtgt
    25081 aacagatcaa acaggcgagc agcgttttta ccgcgtagcg catatagtct ggattggtaa
    25141 aagcgtcggc gaccattgat ggcggtttgg tggcgggcgt tggcggcgta ttcgggtcca
    25201 tctgacccag ttgcaataac gtctggcaga tattctccag gttacattcc cgtgccgcca
    25261 tcgcttcact ttcagtgatc cgccagtcgc tttgccagca ctgaccttcg gcaatggcat
    25321 gctgcagctt gttgatttct gaggctaatt tttgccgaaa ttcaataatt gcctgagaat
    25381 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    25441 cgcagctttg ccaccatgcg ctctgagttc gccagttggc atcgtccgcg aggcaaaaga
    25501 cattgagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    25561 ggctgtccgt caggtggcta atggcatcat caagccgatc attaagcgcc tgatgcatct
    25621 gcgtgatagc gcggctggga aaccacagca cgccgattaa cgtcatcagc aaggttggat
    25681 agaggccgac aacgatacac cacagggtta agcgcacgac cacttccgga tagtcgagca
    25741 tggcggggaa ggtttgcccg taaatagcga ctatggcgac ggcgaaaaag accagcccca
    25801 accgatgggt gcgcatcaaa aacatacagc ccatcaggat cggtccggcg atgatcaatc
    25861 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    25921 ccgtggcaac cacaaacaag atcgcgacaa atttggtgta aaacgcgttc gactgaatac
    25981 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaggtc atcgagatca
    26041 gaattaccag caggcagcct acccagagtt gcacggtctg cggcacgcga cctggccgcc
    26101 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac caccggtaat ggcagggagt
    26161 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    26221 ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    26281 gttaatagaa cgagacactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    26341 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cgctcatcag
    26401 gcgaatggtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc ggaagttggc
    26461 gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg cagaagcaaa
    26521 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    26581 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgcgccac
    26641 cagcgcatcg atgccgctga cggcgctggc ggctgactgc gcctgtaaca atacggcatt
    26701 aagatccgct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt ccgctgagac
    26761 aaaaccttct ttcagtaatg gctcggtgcg gcgtaatgta tctgtggcct gtttcgcggc
    26821 ggcacgggct ttttctaccg tggcattaac cgagtcggca ccaaactgtt gcgcgtcaac
    26881 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cggctttcgc
    26941 cagattggct tcgtatgggc gtgggtcgat gcggaatagc aaatcaccct gtttcaccgc
    27001 ctggttgtcg gtgaccgcca gttctacaat gcgaccgctg acttccggca ccacatcaat
    27061 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    27121 gaaaacaagg gcaaccagcg ccaacgcaac cactaacaga gcagggaatt tactgcgagg
    27181 agcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa atgccagcca
    27241 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    27301 gatatttgcc cgctgaataa ttcgcctcgt gatgagcgtc aaaataaggc tggcaatggc
    27361 gcagaaaaac cagccgggat aataagcgcc gatcaccggg attgccggag ataatgagca
    27421 tcccgaaacc atgaagacgg gaattatcca ggctgttttt ttgagttgca tcgccatgcg
    27481 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    27541 tatcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataaa aatagattta
    27601 ttcagccacc gcattcataa cttatatgtt ttatgtatta atttgttgta tatattttta
    27661 aaataaatgc ttttgggggc ttaagcgccc ccaaaagtga gtagcattaa tttggggtta
    27721 cgatattcac ccacaaatcg aaattatcca gacaggcaat aatttcttcc agtttcgcgc
    27781 cattgccaat aattttggct ttcttcgctt ttaccagatc caccattttg acttgtccgg
    27841 tcagcacggc atgcagatct tcacggctga tgtagaaaga ggcgttggct tcaggttgca
    27901 gcgttttacg gtagttaagc acgctatcgt tcagcgtcag gttgaggtta tcgccgttgc
    27961 tcatattgaa gttcaggctg atatttttac ccgcggcttt cgcactatcg aggcgaacgg
    28021 acataaagtc gaacagcatt tcgaccgaca ttccgcgaat ggtgtccggg gaaccggtgg
    28081 tgccgtggct gaacttatgt accccttcgc gcagctcttt cgcgccggtc aggtagaaac
    28141 cgcgccatgt ggcggactcg gcctgatagc ccagctgttc aaagttatcc gcttgcagat
    28201 tcttcgcgac ctggtcacca gggttggcgg caatcacctg tttcagcagt tctgccgacc
    28261 agcggtaatc accttgcttg ttcgcttctt tcgccaggtt gatgacgcgg gcagaaccgc
    28321 ccagagcttg cacgtaacgt ttacccatct ccacctgacc atacggatgc aggttagccg
    28381 ggttaccgtc gtaatagcca agatagaagt tatacaccgc gcgggcgttg tggctgacag
    28441 aaccgtaata gccgcggctg gcccagttat tggcaagcgc aggcggcagc ttaatcatgt
    28501 cgccgatttc attcatggtg tagccctggt tcgccaggtg cagggtctgg tcgtgaatgt
    28561 acttgatggt atcgcgatat ttaccaatat aatcattgat atgcttattg ccccagaccg
    28621 gccaggtgtg cggcataaac agtacttccg cgtcgttacc ccacatatcc agcgtttcgt
    28681 tcaggtactc ggtccacttg ctggtatcgc gggttttcgc gccgcgcaga gtgtagaagt
    28741 tgtgcagggt atgcgtggcg ttctcggcgg tacacagggc tttcagggcc ggaatataga
    28801 agtgcatttc ggctggcgct tcgctacccg gagtcatcag gaagtcaaat tccaggccgt
    28861 cgataatcat cttctcgcca gttctgacga tagttttagt cggtgcaata atgctcgggt
    28921 cgcccgttgc cagcgtcacg cccaggccgt tgcccacgtt gccttgcgcg ttgtgcggca
    28981 gtaacagacc gtaagagtag agcgcacggc ggctcatgat gttacccgcc agcacgtttt
    29041 cgctgatggc ttcgtccata aagcctgccg gagcaatcac ctgcactttg ccggatttaa
    29101 catcggcttc agagataata cctttcacgc cgccatagtg gtcggcgtgg ctgtgagtgt
    29161 agataacggc aacaatcggt ttttgcggac gatgctggaa gtaaagatca agtgcggctt
    29221 ttgctgcagg cggcgtcacc agcgggtcga taacaataat gcctttctcg ccctcaacga
    29281 acgtaatgtt agagatatcc tggccgcgca cctgatacat tttatcggtg actttgaaca
    29341 ggcctgaaat gccgttgatc tgcgactgac gccacaggct ggggtttatg gtttccggcg
    29401 cagcggcatt aatatcaaat ttgtaatcat tcgcgcggta gtaaatttta ccgttcgcat
    29461 cacgcagaat accttcatcc agcagcggag cgataaatcc acgctgagca tcgtcaaaat
    29521 cctgacgatc gctaaacggt aatgatttgg cgtaattctg attaacctgt tgggtatatt
    29581 gagtggcggc tttcgcttca tctgttgcat tagcggtatt taccatcatg ctgctggcgg
    29641 cagttaacgc aataacaatc ctgctcaaac ggaataaccg agagttattc atataaatct
    29701 ccatttatca tttatgtcgt aaatatgtaa tccatgcaaa atacgtcatt taaagctggc
    29761 gttatcataa ttgggaaatt gcgactttaa taagtagaag tgtgagctga acgcgccatt
    29821 ttattaggca tttgtgctga agagggaaaa gtacagtaga actatcattg ctgggaataa
    29881 tatgggctgt tttatgtaat tattatatat attgtacctg tcgttggcac agcgggacat
    29941 tgcgctactg tgccaacgct ttactcatgg gactttagca caggactgtg gcaaaaaacg
    30001 ttgatgcgcc aatattgctg caccctgagc gccattaaag tcagaagatg aggcggcaat
    30061 aaagcgcaca acctgatacg gcagtggacg gcgcaggtac ttttgggtca tggcaatgag
    30121 agtctcgcgt gggaaggcgg gcatatccat cacgccacca cccagaatca ccgcatcggg
    30181 atcgaacaga ttaatgctgg tggcaatggc tcgtgccgcg ttttcaagca gactctggac
    30241 gaaaggggcg ttttccgcat ggacgaaaag atcgctcaat gggtaatttc ggggctgttg
    30301 ttcgtaccag cgtcttagcg ccattccaga gcaattggtt tccaggcacc cagaattgcc
    30361 acacgcgcag tgttgggtca tatctcccag ggggatatgg cccagttcgc ctgccacacc
    30421 gtgcgcaccc gtccacggcg caccgttcat ccacactgcg aaccccatcc ccgtaccgag
    30481 ataggctgcc agaaccagtt gttgcgtaag gcgattttct actacgtccc aggagagttg
    30541 caggttaacg tcgcgggaaa actcaaccgg acaattcagc gtattttcga gcttatcggc
    30601 gagatcatat aaatctgccg ctgttaacgg caggttaggc gtagaaataa tggtgcgttt
    30661 atctttactg accagcgccg gaaatcccat caccagacca tgacagcgag cgttaaagcg
    30721 cctgagttgc tcgtcaatca tttcgccgat acccgacacc aggtcgggag caataacttc
    30781 tgcggtccgc tttttttcgc agtgtagcgt ttcaccttct gctgtccgca gacaaaagcg
    30841 gatatgcgtt gcccccatat ccacgcccgc tacgacgtta tgctgttttt gcatgaggct
    30901 gtacctcgct ttttgcagcc agaatctgcg cggtcataat tctccatgct tcgtcgatat
    30961 tttccgcatg attaaatagg ccggaagtgc cgacgataaa gacatccgcc cctgccgcca
    31021 ttagtttttc gtaagttgcc tggttgcagg aaccgtccac ctcaatttcg tactccagac
    31081 cttctcgttc acgccatgcc ttcagttcgg caagtttatc cagcatttca ggaatgaacg
    31141 gttgtccggc aaaaccggga tcgacagtca tgaccgtaat tttatcagcc ttatggatat
    31201 agtatttcat ggcctcaacc ggcgtttccg gattaagaat cagccccact ttcatgccat
    31261 gacggcggat ttcatcaatc aggcggaacg cttggccgtt gatggtttcc ggatgcagag
    31321 tgatgaaatc agctcccgca cgcgccagtt gagcaatgta atcctgcgga cgcgttacca
    31381 tcagatggca gtcgagcggt ttactcgcca gtttttttac ctggcttacg aagaacggtg
    31441 agagcgtcag attcggcaca aagtggccgt ccatgatatc gatatgaaag taatcggcat
    31501 ggttgtcgat aaattcgatc tgttctttaa atttcagcag atccatacac attaacgagg
    31561 gggagatttt catgttcaat tccttactta ctgataagac ggtcaagggc gacagccgcg
    31621 ataattaatc cgcccatcac caccagttgg taataggttt gtacctgcaa aatattcaga
    31681 ccgttgttga tggtgccgat gatcaacccg ccaatcacca cagagaaaat gcgccccttg
    31741 ccgccgaaga aactggtgcc gccaatgatg gcgctggcaa tggcataggt ttcaaaaccc
    31801 ataccggcaa gcggttctgc ggcaccgagt cgtgcagttg agacgacgcc tgccagacct
    31861 gcacaaacac cggagatgat aaacaccacc aggatgtgga atttcacgtc aatcccggaa
    31921 tagaacgccg agtttttgtt gccgcccagt gcgtagatgt tgcgcccgag ccgcatacgc
    31981 gttgtcagaa accaaaggat gagcgcgaca attagcgaga agataacggg gacaggtatc
    32041 ccaattacgc tggcggcaaa gaagttcacg aagtcaaatg agaagccgta taccgagttg
    32101 gcatcggaga tcaccagcgt gatcccacgg aaaatagcgt tggtgccaag ggtgataatg
    32161 aacgggtgta gtcccgtcca gttgaccagg cagccgttga tcgcccccag tgcgccgcca
    32221 accagtacac cgccaattag cgccgcgaga aacggatcaa tacctgccaa catcagttta
    32281 gcggtcacca taccggaaag cgccagaatc gcgccaaccg aaaggtcgat accggcgacc
    32341 aggatagcga agaattcccc catgccgatc agtaccgtca cggagctttg cacaaaaatc
    32401 tgggtaatat tattggtggt cagaaaatat tctggcgaca gcgaaccaaa aatggcgacg
    32461 atgatcgcca ggataaaaaa agtaccgtat ttatcccaga acagcgcaaa gttgaacggt
    32521 ttcttctcgc tcgcttcgct ttttactcgt gtggtaaagc ccatgccata atctcctctt
    32581 cgctcatgtc atcgcgattg gtcaggattt gcgtcagtcg tccttcgcag aacacggcga
    32641 tgcggtcgca gacggtgata atttcaggta gttcagatga caccatcagg atgacttttc
    32701 cgtcgtccgc cagttggcgc atcactttgt aaatttcggc tttcgcgcca acgtcgatgc
    32761 cacgggtagg ttcatcgaaa atgatcactt ccgggtgaca gcacagccat ttggagatca
    32821 ggactttctg ctggttaccg ccggagagtt cagtgatgtt ctggtttacc gaatgacatt
    32881 tcagcgccag cagttcgcgc tgggcttcag cggtacgttg ctcgtcaact tcatgaaaca
    32941 accccatcgc gcctttatag ccgccgtctt tcagactgcg gctgatagct atgttctgag
    33001 cgatagaaaa gttggggaaa aagccgttat cccggcggct ttcagtgatg taagccatcc
    33061 cttttttcac ggcatccagg ggagaacgtg gagagatatc tttgccatta agacggattt
    33121 ctccgccagc gcgtttatca acgccaaaca ggcagttcat cagttcagta cgtccggaac
    33181 cgaccagtcc ggcaaagcct aatatttctc cccggcagac gctaaatgag atatcccgga
    33241 ccttttttct gtcacggctg gtgacgttgc gcacctcaaa aaccgtatcg tgcgcaaggt
    33301 tgctgacatt ctccttcatc gcgttaaaac ggttttgcag ttcgcgacct accatcagac
    33361 ggacgatatc gtcatttgac acatcgctca ccatgccgct gcaaacgctg ctgccgtctt
    33421 tcatcaccgt atagcggtcg caaatacggc gaatttccgc caatttatgc gagatataga
    33481 cgatggccgt accctcctta cgcaactgat tcatgatcag aaacaaatag tccacctctt
    33541 tattagtgag tgaggaggtg ggttcatcca tgatgatgac tttggcgtcg agcatcagcg
    33601 ttttggcaat ttccagcatc tgcttgtggc tgatagataa attcgccact ttctcatcta
    33661 aatcaacttt caagcccacg cgtaataaca tcatggcggc gcggacacgc atttctcgcc
    33721 agtcgataat attgacgcca cagatttttt tcgtcagatg acgaccaata tataaatttt
    33781 ccagtacggt taattcatca ataacgctga gttcttgata aataatcccg atgccgagtt
    33841 gtgccgctaa tttatgatcc agtttgttat agttaatgtt attaatggta atggtgcctt
    33901 tggtcggctc atgtattccg gataaaactt tcattagcgt ggatttaccc gcgccatttt
    33961 ctcctagtaa tgcatgtatt tcaccaggat aaaccgttaa attaaccgac tttaatgcgt
    34021 gaaccggacc aaaggacttg ccgatccccg ccatcgatat atatggcgtg gccataaata
    34081 acctctctgc acgaaatatc gtattattgc ctgatgggct acacttatca ggtctacgtt
    34141 caggttattt tgttggccgg ataagacact tcattagcat caccatccgg catcatttag
    34201 ttttattgag tgaccaggat tgaatcgacc agtttaaatt ccggtgcttt atccagcggg
    34261 ataaccttgc cggatttttc agcgtcaacc atcagcttca gacccgttgc gccgatatcc
    34321 gccgggttct gggcaaccgt cgcggtcatt tgtccggctt ccaccatttt gcgggcttcc
    34381 ggaatgccat ctgtaccgac gaccagcact tttcccgttt ttccggcgtt tgcgacagcc
    34441 tgagcaacac ccattgccat cgtgtcattc gcgcaataga tcgctttaat attcggatta
    34501 cgttgcaaca cgttagtggc gacatccagt gctttaatac ggtcccagtc ggcaggctgg
    34561 ctggcgacaa gcttgatctg gcttgctttt ttgaaggctt cggtggcacc attacgacgc
    34621 gtttcaccgg aggcgttacc ggctttaccc tcaatgattg cgacttcacc cccttcggca
    34681 cccagcttgt caataatgaa tgacgcgcct ttcgccccga cggcaacatt atcggtggtg
    34741 acaaaacctt ccacattacc gcctgctttt ttcagattat ccatgtcgat tttttcatcg
    34801 agattgacca gataaatgcc ttttttccat gcgcgggcga caggcatgac caggttcact
    34861 gaggataatg gtgcgaaggc gatacctttg taatttttat tactgagatc ttcaaataac
    34921 tgcaattgag attgaaaatc gccttctgaa ggagaggcaa aaatatcaac gctgacgccc
    34981 agcgtttttg cttcatcttc aatgcctttt ttcatatcta cccaaaatgg attggagagt
    35041 gttttcaata cgacggcata ttcggcggca gcaaaagcgc tggttgacaa cattaagccc
    35101 acgagtgtgc cgctgaaata tttcagatat ttattcataa tcatgttctc acggtaaggg
    35161 gataggtaac tccgcgatgg cggaattacc tttttcttat ttcagcgcgc ctggtgagaa
    35221 gaaatcaaca attgcgccgg ttttttgcat attaatatta gcttgttcaa tgttgagctg
    35281 ggcgacagag acaaaaaacg cgtccagcaa agttagttgt aatattcttg ccgaggcatt
    35341 acgacctaat aacggtgttt ccggggccgg tgagcaaata atataatcgg ccagcttcgc
    35401 tatcggtgaa tggtagctat gcgttataca aataatcttt gccccgttct tttttgccag
    35461 ttctacagcc gcttttacat cactggttcg cccggaatgg gtcaccacca gcaccacatc
    35521 tccttcctgt aacaacgaag cagacatcat catgatgtga gcgtccggat aggcctggca
    35581 gcgtacgcca atgcgcaaaa acttgtgctg tacatcagca cagattgcat ttgatcctcc
    35641 ggcaccgtac aaatcccgct gtctggcctg atagaaaaag cgggcggcac ggtggatctc
    35701 atcaacgttg acgatcgact gaccttccat aatggtgcgt aaagtgatgt taaatacctt
    35761 attcaccaca tcctgcggcg cttcatcaaa agccaactcg gaaggcaata cttgttctga
    35821 ctgagaaaaa taatcttcca gcgcactgcg taagttacga aagccgctaa accccagcag
    35881 ctttgatacc ttaacgatca tcgcttcaga taccgccaga gcttctgcga catctttaat
    35941 tgcgggtgca caactcaggt taccgggttt gagtaaccac tccacgatgc ggctttcgtt
    36001 ttctgtcatt ccttcctgct tcattcgcag gtaaggcgct aacccgatac cgttcggaag
    36061 cgctgaatca aactctgact ggctcatcac gttctcttgt ccttgaattg aagacgccac
    36121 tatatcacca cagcaagaac agaaaagatg ctggcaactt cacaaaaaaa taaa
//
LOCUS       DAZGHF010000034        36118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGHF010000034 DAZGHF010000000
VERSION     DAZGHF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.034"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(59..1150)
                     /gene="ychF"
                     /locus_tag="ACHK3A_001813"
     CDS             complement(59..1150)
                     /gene="ychF"
                     /locus_tag="ACHK3A_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO8739900.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEEREEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1267..1851)
                     /gene="pth"
                     /locus_tag="ACHK3A_001814"
     CDS             complement(1267..1851)
                     /gene="pth"
                     /locus_tag="ACHK3A_001814"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO8739901.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEIWFTDGLTKATNRLHAFKAQ"
     gene            2129..2407
                     /gene="ychH"
                     /locus_tag="ACHK3A_001815"
     CDS             2129..2407
                     /gene="ychH"
                     /locus_tag="ACHK3A_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO8739902.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2462..4141)
                     /gene="dauA"
                     /locus_tag="ACHK3A_001816"
     CDS             complement(2462..4141)
                     /gene="dauA"
                     /locus_tag="ACHK3A_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO8739903.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4266..5213)
                     /gene="prs"
                     /locus_tag="ACHK3A_001817"
     CDS             complement(4266..5213)
                     /gene="prs"
                     /locus_tag="ACHK3A_001817"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO8739904.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5364..6215)
                     /gene="ispE"
                     /locus_tag="ACHK3A_001818"
     CDS             complement(5364..6215)
                     /gene="ispE"
                     /locus_tag="ACHK3A_001818"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO8739905.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGAEISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6215..6838)
                     /gene="lolB"
                     /locus_tag="ACHK3A_001819"
     CDS             complement(6215..6838)
                     /gene="lolB"
                     /locus_tag="ACHK3A_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO8739906.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPSNMELTDGGQRIKLKMDNWIVK"
     gene            7052..8308
                     /gene="hemA"
                     /locus_tag="ACHK3A_001820"
     CDS             7052..8308
                     /gene="hemA"
                     /locus_tag="ACHK3A_001820"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO8739907.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDGLGLE"
     gene            8350..9432
                     /gene="prfA"
                     /locus_tag="ACHK3A_001821"
     CDS             8350..9432
                     /gene="prfA"
                     /locus_tag="ACHK3A_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO8739908.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9432..10265
                     /gene="prmC"
                     /locus_tag="ACHK3A_001822"
     CDS             9432..10265
                     /gene="prmC"
                     /locus_tag="ACHK3A_001822"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO8739909.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIHAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10262..10654
                     /gene="sirB2"
                     /locus_tag="ACHK3A_001823"
     CDS             10262..10654
                     /gene="sirB2"
                     /locus_tag="ACHK3A_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO8739910.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10658..11467
                     /gene="sirB1"
                     /locus_tag="ACHK3A_001824"
     CDS             10658..11467
                     /gene="sirB1"
                     /locus_tag="ACHK3A_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO8739911.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11503..12357
                     /gene="kdsA"
                     /locus_tag="ACHK3A_001825"
     CDS             11503..12357
                     /gene="kdsA"
                     /locus_tag="ACHK3A_001825"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO8739912.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12506..12613)
                     /locus_tag="ACHK3A_001826"
     CDS             complement(12506..12613)
                     /locus_tag="ACHK3A_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8739913.1"
                     /translation="MTLAQFTVIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13018..14118)
                     /gene="chaA"
                     /locus_tag="ACHK3A_001827"
     CDS             complement(13018..14118)
                     /gene="chaA"
                     /locus_tag="ACHK3A_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO8739914.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            14388..14618
                     /gene="chaB"
                     /locus_tag="ACHK3A_001828"
     CDS             14388..14618
                     /gene="chaB"
                     /locus_tag="ACHK3A_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO8739915.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14776..15471
                     /gene="chaC"
                     /locus_tag="ACHK3A_001829"
     CDS             14776..15471
                     /gene="chaC"
                     /locus_tag="ACHK3A_001829"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO8739916.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGLA"
     gene            complement(15515..15868)
                     /gene="ychN"
                     /locus_tag="ACHK3A_001830"
     CDS             complement(15515..15868)
                     /gene="ychN"
                     /locus_tag="ACHK3A_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO8739917.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16054..17448
                     /gene="ychO"
                     /locus_tag="ACHK3A_001831"
                     /gene_synonym="iatA"
     CDS             16054..17448
                     /gene="ychO"
                     /locus_tag="ACHK3A_001831"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO8739918.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDAFSQQVNQHV
                     ESWLSAWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(17449..18099)
                     /gene="narL"
                     /locus_tag="ACHK3A_001832"
     CDS             complement(17449..18099)
                     /gene="narL"
                     /locus_tag="ACHK3A_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO8739919.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18092..19888)
                     /gene="narX"
                     /locus_tag="ACHK3A_001833"
     CDS             complement(18092..19888)
                     /gene="narX"
                     /locus_tag="ACHK3A_001833"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO8739920.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSARFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            20227..21618
                     /gene="narK"
                     /locus_tag="ACHK3A_001834"
     CDS             20227..21618
                     /gene="narK"
                     /locus_tag="ACHK3A_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO8739921.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            22011..25754
                     /gene="narG"
                     /locus_tag="ACHK3A_001835"
     CDS             22011..25754
                     /gene="narG"
                     /locus_tag="ACHK3A_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO8739922.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLMGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQALDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITSQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            25751..27289
                     /gene="narH"
                     /locus_tag="ACHK3A_001836"
     CDS             25751..27289
                     /gene="narH"
                     /locus_tag="ACHK3A_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO8739923.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            27286..27996
                     /gene="narJ"
                     /locus_tag="ACHK3A_001837"
     CDS             27286..27996
                     /gene="narJ"
                     /locus_tag="ACHK3A_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO8739924.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            27996..28673
                     /gene="narI"
                     /locus_tag="ACHK3A_001838"
     CDS             27996..28673
                     /gene="narI"
                     /locus_tag="ACHK3A_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO8739925.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(28858..28989)
                     /locus_tag="ACHK3A_001839"
     ncRNA           complement(28858..28989)
                     /ncRNA_class="other"
                     /locus_tag="ACHK3A_001839"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(29036..29120)
                     /locus_tag="ACHK3A_001840"
     tRNA            complement(29036..29120)
                     /locus_tag="ACHK3A_001840"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29084..29086),aa:Tyr,seq:gta)
     gene            complement(29155..29239)
                     /locus_tag="ACHK3A_001841"
     tRNA            complement(29155..29239)
                     /locus_tag="ACHK3A_001841"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29203..29205),aa:Tyr,seq:gta)
     gene            complement(29399..30241)
                     /gene="purU"
                     /locus_tag="ACHK3A_001842"
     CDS             complement(29399..30241)
                     /gene="purU"
                     /locus_tag="ACHK3A_001842"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO8739926.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(30291..30749)
                     /gene="ychJ"
                     /locus_tag="ACHK3A_001843"
     CDS             complement(30291..30749)
                     /gene="ychJ"
                     /locus_tag="ACHK3A_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO8739927.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAATLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GSKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            30862..31767
                     /gene="rssA"
                     /locus_tag="ACHK3A_001844"
     CDS             30862..31767
                     /gene="rssA"
                     /locus_tag="ACHK3A_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO8739928.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            31859..32872
                     /gene="rssB"
                     /locus_tag="ACHK3A_001845"
     CDS             31859..32872
                     /gene="rssB"
                     /locus_tag="ACHK3A_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO8739929.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            33074..33982
                     /gene="galU"
                     /locus_tag="ACHK3A_001846"
     CDS             33074..33982
                     /gene="galU"
                     /locus_tag="ACHK3A_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO8739930.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(34127..34540)
                     /gene="hns"
                     /locus_tag="ACHK3A_001847"
     CDS             complement(34127..34540)
                     /gene="hns"
                     /locus_tag="ACHK3A_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO8739931.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            35144..35761
                     /gene="tdk"
                     /locus_tag="ACHK3A_001848"
     CDS             35144..35761
                     /gene="tdk"
                     /locus_tag="ACHK3A_001848"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO8739932.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(36044..>36118)
                     /locus_tag="ACHK3A_001849"
     CDS             complement(36044..>36118)
                     /locus_tag="ACHK3A_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739933.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT         8891 a         8871 c         9477 g         8879 t
ORIGIN      
        1 ccattatgtg cgactcacat aatggctcat tattaaccgt cagggtatta cgtggaattt
       61 agacgttgaa caggaagttc atcacatcgc catctttaac gatgtaatct ttaccttctg
      121 cacgcatttt gcctgcttct ttcgcgcctt gttcaccttt gtaagtgatg aaatcttcaa
      181 acgagatagt ctgtgcgcgg ataaagcctt tttcgaaatc ggtatggatt ttgccagccg
      241 cttgcggtgc agttgcgccg acggggatgg tccatgcgcg cacttctttc accccagcgg
      301 tgaagtaagt ttgcaggttc agcagtttat aaccggcacg gatcacacgg ttcaggcccg
      361 gctcttccag tcccagttcc tgcataaact cttcacgttc ttcatcgtcc agttcggcaa
      421 tgtctgcttc aacagctgca caaaccggaa ccacaacaga accttctttc gccgcgattt
      481 cacgcacctg gtcaagatac gggttgtttt caaaaccgtc ttcgttgacg ttggcgatgt
      541 acatcgtcgg tttcagcgtc aggaagctca ggtaacgaat agccgctttt tcttctgcgc
      601 ttaaatccag cgcgcgcagc atgcccgcgt tttccagctg tggaaggcat ttttccagta
      661 ctgccagttc ggctttcgcg tctttatcgc cgcctttggc tttcttctgt acgcggtgaa
      721 tcgcgcgctc gcaggtgtcg agatccgcca gtgccagttc ggtattgata acttcaatat
      781 cgtccgccgg gttaacttta ccggaaacgt gaatgatatt gtcattctca aagcagcgaa
      841 caacgtggcc gatcgcttcg gtttcacgaa tgttggtcag gaactggtta cccagacctt
      901 cacctttcga cgcgcccttt accagaccgg cgatatcgac aaattccatg gtagtgggaa
      961 gcgtacgctg cggttttacg atttcagcca gttgatccag gcgagggtca ggcattggta
     1021 cgacgcccgt gttcggctca atggtacaga atggaaagtt ggccgcttca ataccggctt
     1081 tggtcagcgc gttgaacagg gtagatttcc cgacgttggg caaaccgacg ataccgcatt
     1141 tgaatcccat gatttaaatc accttaatat cttaataatc aacctgttat tgctaacaga
     1201 ttgcagaaat ggaaataact ttgcctatta tacacggcac tcggcaaaaa tgccgcagac
     1261 aacgacttat tgcgctttaa aggcgtgcaa tcggttcgtt gctttggtca agccatctgt
     1321 aaaccatatt tcagtacaac gtgccgcttc gtcgatggct tcatcaatta acttctgttc
     1381 actaacaggc ggtttgccta acacaaaacc gacaacttta tttttatcgc ctggatgacc
     1441 aattccgatg cgtaaacggt gaaagttagg gttattaccc aatttactga tgatgtcttt
     1501 cagtccattg tgaccaccat ggccaccgcc caacttaaat ttggcgacgc caggaggcag
     1561 atccagttcg tcgtgggcca ccagaatctc gtccggatta atgcggaaaa aactggccat
     1621 cgccgcaacg gctttgccgc tgagattcat aaatgtagtc gggactaaca ggcggacatc
     1681 ttcgcctcca agggtgactc gcgaagtata accaaagaat ttagcctctt cgcgcagcgg
     1741 agcgcgcaat cgctctgcca gtaagtcaac gaaccaggca ccagcattat gtcgcgttgc
     1801 ggcgtattca gcgccggggt tcgccaggcc gacaatcaat ttaatcgtca cgtttttttg
     1861 tcctgagtgt gtacataact ggcgcgtagt ttactgtttg cggccccgct tgacaaaaaa
     1921 ctgcgtatca aatgcagata acgcaataat tgcctaagtg gactattaga aagtcaaggt
     1981 gttcaggcgt ttatttgtaa agttttgttg aaataagggt tgtaattgtg atcacgcccg
     2041 cacataaccc acagggtgtt gtctatactt tacacataag gaagaggggt attccctgtt
     2101 acaacccaga aagttccgga ggtgacatat gaaacgcaaa aacgcttcgt tactcggtaa
     2161 cgtgctcatg gggttgggtc tggtggtaat ggtggtcggc gtggggtatt caatcctcaa
     2221 ccagttacca cagtttaata tgccccagta tttcgcacat ggtgcagtgc taagtatttt
     2281 cgtcggtgcc attctctggc tggcgggtgc ccgtgttggc gggcatgaac aggtgtgcga
     2341 ccgttactgg tgggttcgcc actatgacaa acgttgccgc cgtagcgata atcgccgtca
     2401 tagctaacaa ataatgcggt ttgctgacca gtcagtttgc gctgactggt caatttcgta
     2461 cttataaatc cgccatcgcc gcgcgacgat tcgggaagaa cgccaggcgt cccgggatcg
     2521 gttgaatgcc tgcgcgagcc atagtgcgca gtggctggaa ttccacgttg cacacgcgca
     2581 gttcacatcc ttcgggtaga cgcttcacaa aacgctggaa cgcatcaaga ccaccagcat
     2641 caagtaccgg aacggcatcc cacttcagaa tcacaatccg tttgccttca agacgtgact
     2701 ccaggtccgt gaacaagcct tcagcggcag caaagaacag cgggccaata acgcgcagaa
     2761 ccagaacatc gtctggaacg tctacgacta ccggtgccag gcgagtcata cgtgcgatac
     2821 gacgcataaa cagtagcgat gccagcacga tccccacgct gatggcaata accatatcaa
     2881 acagcacggt cagcgacatg cacagcagca tgacgatgat gtcatctttc ggcgcatgac
     2941 gcagcaagtc gaccactttg tgcgcttcac tcatgttcca cgccaccatc aacagcaggg
     3001 cagccatggc ggaaagaggc agccaggaga gcagcggtgc cagtaccagc agggcaagaa
     3061 taaccagaat agagtggatc accgccgaga taggggaagt tgccccggca cggacgttag
     3121 cggcagaacg cgcgatggca gctgtagcgg taataccacc aaagaacgga gcgatgatat
     3181 tccccagtcc ctgtccaacc agttcactgt tcgctttgtg tttcgtcccg gtcataccat
     3241 ccagcaccac ggcgcagagc agtgattcaa ttgcccctaa catcgccatc gaaaatgctg
     3301 ccggaagcag agtgcgaatg gagtcccagg tgagggtaaa ttcggaattg ggcaggtccc
     3361 acggcagtac cagttgcggc agcagttgcg gaataccgtt accctgagaa ccatcggcca
     3421 gaacgtagtg gaattgcgaa ccgatggtag caacatgtcc gccgagcagg ttaacaatcc
     3481 ccatcaccgc gcaaccagcc agcaaggccg gaaggtgacc gggtaaacga atgcccagac
     3541 gtggccagaa aaccagaatg ccaagcgtca caatgccaat ggcagcatca cccacattaa
     3601 tggtcggcaa cgccataaat aatgcgccga ctttttgtag ataatgttca gggacatggg
     3661 ccatttgcag accgagaaaa tctttaatct gcatggtacc gatggtgatc ccgataccgg
     3721 aagtaaaacc taaggtaacg gaaaccggaa tatattcaat caagcgtcca aaacgtgcca
     3781 gccccatcag gatcaaaaag acacctgaca tgagtgtcgc gaccagcagt cctgccagtc
     3841 caaactgttg cgaaacggga tagagaatta ccacaaatgc cgcagtcgga ccggaaacgc
     3901 taaagcgtga cccacccgtc agagcaatga caatccccgc aacagctgcg gtatataaac
     3961 cgtactgggg tgccacacca ctaccaatag ccaacgccat tgccagcggg atagcaataa
     4021 tcccgacggt tatcccggca atcaggtcac gggtaaaccg tgcggcagta tatttttctt
     4081 tccagcaagc gtcgatcaga gcgcggaaag gcatcacatg tgaggaaaat attttgttca
     4141 caataatgtt tcatccgtga gcgcatcatc tgtcaactaa atggcaggtg aaggaggcat
     4201 aggtcataca aatggatatt acagacaaaa aaacccgccg cagcgggtct ttgagccggg
     4261 ttcgattagt gttcgaacat ggcagagatc gattcttcgt tgctgatacg acgaatcgct
     4321 tcggccagca tacctgacag ggtcagagta cgcacgttcg gcagtgattt gatttcatcg
     4381 ctcagcggaa tggtatcgca gacaacgact tcatcaatta cagagttacg caggttgttc
     4441 gccgcgttgc cagagaagat cgggtgagtc gcgtacgcaa atacacgttt agcaccacgt
     4501 tctttcagag cttcagcagc tttacacagc gtaccgccag tatcgatcat atcatcgacc
     4561 agtacgcagt cacgacctgc aacgtcaccg atgatatgca tcacctggga aacgttcgca
     4621 cgcggacgac gtttgtcgat gattgccata tcggtatcgt tcagcagctt agcgatagcg
     4681 cgggcacgca caacgccgcc gatgtccgga gaaaccacaa ttgggttatc cagattcagc
     4741 tgcagcatgt cttccagcag gatcgggcta ccaaatacgt tatcaaccgg aacgtcgaag
     4801 aaaccctgaa tctgttcagc gtgcagatcc actgtcagca cacggtcaac accgacgctg
     4861 gagaggaagt ctgcaaccac tttcgcagtg attggtacac gagcggaacg gacgcgacgg
     4921 tcctggcgcg catagccaaa gtaggggata acagcggtga tacgacctgc ggaagcacga
     4981 cgcagggcat caaccataac gactaattcc atcaggttgt cgttagtagg ggcacaagtg
     5041 gactggatga tgaaaatatc accaccgcgt acattttcgt taatttgtac gctgacttcg
     5101 ccgtcgctaa agcgacctac agcggcgtcg ccgagtgaag tgtacaggcg gttggcaata
     5161 cgttgtgcta gttccggggt ggcgttacca gcaaaaagct tcatatcagg cacgagaaga
     5221 acctcaggca tgcgtccatt ggtggaaaga atctgccgaa aactgtgcgg gccaggcatg
     5281 attctttcca ggcggtgtat taaagagcgc gatgcaacgt ctggaacaag gtgacgttgt
     5341 caccgaaact cagcttgccc ggcttaaagc atggctctgt gcaatgggga aagattaacg
     5401 cctttcgcca caaagccatt gagccattcc ggggcttgct ctagcacctg gcgggcttca
     5461 gactctgtat caaattcagc aaagacacag gcccctgtcc cagtcaggcg cgacggggcg
     5521 tattctaaca gccaggaaag caccgcatca acctcgcgaa aacgttttct tgcgataacc
     5581 tcgcaatcat tgctgaattc acattttagc aacgtttcta ttgacctttt tggcgtattg
     5641 cgcgggagtt caggatcttt aaaaatcacc ggtgtcggaa tacttacacc agggtgcgcc
     5701 accaagtacc acttctctgg cggatccacc ggcgttagta tttcaccaac gccttcggca
     5761 aacgcggcat gcccccgaac aaagacagga acatctgcgc ccagcgtcag ccccatttcc
     5821 gccagctcat ccatgcttag cccgcattgc cagagatggt ttaatgccac cagcaccgtc
     5881 gcggcattgg atgaaccacc acccagaccg ccgcccatcg gcaaacgctt gtcaatactg
     5941 atttctgcac cgcttcccgt aggaagacgc ccgctgtctg ccgctgtttt catcagcaac
     6001 cgtgctgcgc gaacgatcag gttatcttca tgttccacgc cttcaacggg tgttaacaga
     6061 cgaatatccc catcgtcacg aagctcaatg ctgatggtgt cgccgtaatc caggaactga
     6121 aacagcgttt gcagcgtgtg gtaaccgtcc gcacgctgac cggtaatgta taaaaacaga
     6181 ttaagttttg ccggagaggg ccactgtgtc cgcattattt cactatccag ttatccattt
     6241 ttaacttgat gcgctgacca ccatcggtga gttccatatt ggatggcatc gcaggttgag
     6301 tttttgtgtc ataaccaccg taaacaactt tccagttttt gccattctgg ctgtaggtga
     6361 tttcgctaag gcggtactgg tcgtccagtt tgtagtcagt cgcatcaccc ggcaaaccta
     6421 aaatccactg gcgtaagctg ttgagtggaa tgggcattcc ggtcaatttg ccaatcatct
     6481 cttcggcgtc atcggcggta taacgctggc ctttattgtc gactaactgc acgttacccg
     6541 gttgagcatt cagctccagt tccgtgctgc ccaatgggtt agtcagcagc agacggtagc
     6601 gatcctggcc agtttgctgc cagaagaaac gggcgtacac tttttgttgg tcagaaatat
     6661 aagcgaacgc gccgcgagtt tgatactgat taagattgcg cacgtcttgc tgatgctgac
     6721 gccattgtgg cgaatccggg cttttgccag gacctttggg cgtggtaacg gaacaggcag
     6781 tgagcacaag agcagccagc ggtagtaggc ggataagacg aaaatcgggc aggggcatag
     6841 tgatgacaag tccttgagat acgttgcagt tataaccctt aatgctagcg ttaccgtccg
     6901 ctatcgtcta tgttcaagtt gtcttaattg ccagaatcta acggctttcg gcaattactc
     6961 caaaaggggg cgctctcttt tattgatctt acgcatcctg tatgatgcaa gcagactaac
     7021 cctatcaacg ttggtattat ttcccgcaga catgaccctt ttagcactcg gtatcaacca
     7081 taaaacggca cctgtatcgc tgcgagaacg tgtatcgttt tcgccggata agctcgatca
     7141 ggcgcttgac agcctgcttg cgcagccgat ggtgcagggc ggcgtggtgc tgtcgacgtg
     7201 caatcgcacg gaactttatc ttagcgttga agagcaggat aacctgcaag aggcgttaat
     7261 ccgctggctt tgcgattatc acaatcttaa tgaagaagac ctgcgtaaaa gcctctactg
     7321 gcatcaggat aacgacgcgg ttagccattt aatgcgtgtt gccagcggcc tggattcact
     7381 ggttttgggg gagccgcaga tcctcggtca ggttaaaaaa gcgtttgccg attcgcaaaa
     7441 aggccatatg aaggccagcg aactggaacg catgtttcag aaatctttct ctgtagcgaa
     7501 acgtgttcgc actgaaacag atatcggtgc cagcgctgtt tctgtcgctt ttgcggcttg
     7561 tacgctggcg cggcagatct ttgaatcgct ctctacggtg acggtactgc tggtgggtgc
     7621 gggcgaaacc atcgagctgg tggcgcgtca tctgcgcgaa cacaaagtac agaagatgat
     7681 tatcgccaac cgcactcgcg aacgtgccca aattctggca gatgaagtcg gcgcggaagt
     7741 tattgccctg agtgatatcg acgaacgtct gcgcgaagcc gatatcatca tcagttccac
     7801 cgccagcccg ttaccgatta tcgggaaagg catggtggag cgcgcattaa aaagccgtcg
     7861 caaccaacca atgctgttgg tggatattgc cgttccgcgc gatgttgagc cggaagttgg
     7921 caaactggcg aatgcttatc tttatagcgt ggacgatctg caaagcatca tttcgcacaa
     7981 cctggcgcag cgtaaagccg cagcggttga ggcggaaact attgtcgctc aggaaaccag
     8041 cgaatttatg gcgtggctgc gtgcacaaag cgccagcgaa accattcgcg agtatcgcag
     8101 ccaggcagag cacgttcgcg atgagttaac tgccaaagcg ttagcggccc ttgagcaggg
     8161 cggtgacgct caagccatta tgcaggatct ggcatggaaa ctgactaacc gcctgatcca
     8221 tgcgccaacg aaatcacttc aacaggccgc ccgtgacggg gataacgaac gcctgaatat
     8281 tctgcgcgac ggcctcgggc tggagtagca gtacatcatt ttcttttttt acagggtgca
     8341 tttacgccta tgaagccttc tatcgttgcc aaactggaag ccctgcatga acgccatgaa
     8401 gaagttcagg cgttgctggg tgacgcgcaa actatcgccg accaggaacg ttttcgcgca
     8461 ttatcacgcg aatatgcgca gttaagtgat gtttcgcgct gttttaccga ctggcaacag
     8521 gttcaggaag atatcgaaac cgcacagatg atgctcgacg atcctgaaat gcgtgagatg
     8581 gcgcaggatg aactgcgcga agctaaagaa aaaagcgagc aactggaaca gcaattacag
     8641 gttctgttac taccaaaaga tcctgatgac gaacgtaacg ccttcctcga agtccgtgcc
     8701 ggaaccggcg gcgacgaagc ggcgctcttc gctggcgatc tgttccgtat gtacagccgt
     8761 tacgccgaag cccgccgctg gcgtgtagaa atcatgagcg ccagcgaggg tgaacatggt
     8821 ggttataaag agatcatcgc caaaattagc ggtgatggtg tatatggtcg tttgaaattc
     8881 gaatctggcg gtcatcgcgt gcagcgtgtg cctgctacgg aatcgcaggg tcgaattcat
     8941 acttccgctt gtaccgttgc ggtaatgcca gaactgcctg acgcagaact gccggacatc
     9001 aacccagcag atttgcgcat tgatactttc cgctcgtcag gggcgggtgg tcagcacgtt
     9061 aacaccaccg attcggcaat tcgtattact cacttgccga ccgggattgt tgttgaatgt
     9121 caggacgaac gttcacaaca taaaaacaaa gctaaagcac tttctgtact cggtgctcgc
     9181 atccacgctg cagaaatggc aaaacggcaa caggccgaag cgtctacccg tcgtaacctg
     9241 ctggggagtg gcgatcgcag cgaccgtaac cgtacttaca acttcccaca ggggcgcgtt
     9301 accgatcacc gcatcaacct gacgctctac cgcctggatg aagtgatgga aggtaagctg
     9361 gatatgctga ttgaaccgat tatccaggaa catcaggccg accaactggc ggcgttgtcc
     9421 gagcaggaat aatggaatat caacactggt tacgtgaagc aataagccaa cttcaggcga
     9481 gcgaaagccc gcggcgtgat gctgaaatcc tgctggagca tgttaccggc agagggcgta
     9541 cttttattct cgcctttggt gaaacgcagc tgactgacga acaatgtcag caacttgatg
     9601 cgctactgac gcgtcgtcgc gatggtgaac ccattgctca tttaaccggg gtgcgagaat
     9661 tctggtcgtt gccgttattt gtttcgccag cgaccttaat tccgcgcccg gatacggagt
     9721 gtctggtgga gcaggcactg gcgcggttgc ctgaacaacc ttgccgtatt ctcgatctcg
     9781 ggacgggtac cggggcgatt gcgctggcgc tggccagcga gcgtccggac tgcgaaatta
     9841 ccgctgtaga tcgtatgcca gatgctgtcg ccctggcgca acgtaatgcc cagaatctgg
     9901 cgatcaaaaa catccatatt ctgcaaagcg actggtttag cgcgctagcc gggcagcagt
     9961 ttgcgatgat tgtcagtaat ccgccgtata ttgacgagca ggacccacat cttcaacaag
    10021 gcgatgtccg ctttgagccg ctcactgcgc tggttgcggc agacagtgga atggcagaca
    10081 tcgtgcatat catcgaacag tcgcgtaacg cgctggtatc cggcggcttt ctgcttctgg
    10141 aacatggttg gcagcagggc gaagcggtgc gacaggcatt tatccacgcg ggatatcatg
    10201 acgtcgaaac ctgtcgggac tatggtgata acgagcgcgt aacgctcggc cgctattatc
    10261 aatgacaagt ttttctacac tgcttagtgt tcatcttatt agtatcgcgc tttcagttgg
    10321 gctattaacc ttacgtttct ggctacgtta tcagaagcat ccacaggcat ttgcgcgctg
    10381 gacgcgcatt gtgccgccgg ttgtcgatac gctgttattg ttaagcggca ttgcgttgat
    10441 ggctaaagcg cacatcctgc cattttccgg gcaggcacag tggctgactg aaaagctgtt
    10501 tggagttatc atttatatcg ttttgggttt tattgcactc gattatcgtc gtatgcacag
    10561 tcagcaggcg cgcattattg ccttcccgct ggcgttggtg gtgctgtaca tcatcattaa
    10621 actcgccacc acaaaagtac cgttactggg gtaagtcatg agatcgttag ctgatttcga
    10681 atttaataaa gcgccattgt gcgaaggcat gatcctggct tgcgaagcaa tccgccgcga
    10741 ttttccctcg caagatgttt acgacgaact ggagcgtctc gttagtctgg cgaaggaaga
    10801 aatcagccag cttctgcctt tagaagagca gctggaaaaa ctgatcgcgc tgttttacgg
    10861 cgactgggga tttaaagcct cacgcggcgt ttatcgtctt tccgatgcat tatggctgga
    10921 ccaggtgtta aagaatcgac agggcagtgc ggtatcatta ggtgccgttt tattatgggt
    10981 cgcgaatcgt cttgatttgc cgctgctgcc ggtgattttc cctacgcagc tgatattgcg
    11041 cattgaatgt ccggatggcg aaatttggct gattaatcct tttaacggtg aatcgttaag
    11101 cgaacatatg ctggacgtat ggttaaaggg aaatatcagc ccgtcggcgg aactgtttta
    11161 tgaagacctt gatgaagctg ataacattga ggtaatccgc aaattgctgg atacactcaa
    11221 agcttcgttg atggaagaga atcagatgga gctggcgtta cgcaccagcg aagctttatt
    11281 acaattcaac ccggaagatc cctatgaaat tcgcgatcgc gggttgattt atgcgcaact
    11341 ggattgcgaa cacgttgcgt tgaacgattt aagttatttc gttgaacagt gtccggaaga
    11401 cccgatcagc gaaatgatcc gtgcgcaaat aaataacatc gcgcataaac atattgtgct
    11461 gcattaatta atcgacattt tactcaagat taaggcgatc ctatgaaaca aaaagtggtt
    11521 agcattggcg acatcaacgt agcaaatgac ctgccgttcg tactgtttgg cggtatgaac
    11581 gtgctggaat ctcgcgatct ggcgatgcgc atttgcgagc actacgtaac cgtgacccag
    11641 aaactgggta tcccttacgt gttcaaagcc tcttttgaca aagccaaccg ctcctccatc
    11701 cactcttatc gtggaccggg cctggaagaa gggatgaaaa tcttccagga gttgaagcag
    11761 acttttggtg tgaaaattat caccgacgtt cacgaaccaa gtcaggcaca gcccgttgcg
    11821 gatgtcgtgg atgtgattca gttgccggcg tttcttgctc gccagactga cctggtggaa
    11881 gccatggcga aaaccggtgc ggtaattaac gtcaagaaac cgcagtttgt tagcccggga
    11941 cagatgggta atatcgttga taaatttaaa gaaggcggca acgaaaaagt gattctttgc
    12001 gatcgcggtg ctaacttcgg ctatgacaac ctggttgtcg atatgctggg cttcagcatc
    12061 atgaagaaag tgtctggtaa ttcgccggtg attttcgacg tgacccacgc actgcaatgc
    12121 cgcgatccgt ttggcgcagc ttccggcggt cgtcgtgctc aggtggctga gctggcacgt
    12181 gccggtatgg cggtaggtct ggcggggctg tttattgaag cgcatccgga tccggaacat
    12241 gcgaaatgtg atggtccatc cgcgctgccg ctggctaaac tggaaccgtt cctcaagcag
    12301 atgaaagcga ttgatgactt ggtgaaaggt ttcgaagaac tggataccag caagtaatct
    12361 tttttgcctg aaaactaaag ccttagcgtt ctgcgttaag gcttttttca tgggggccgg
    12421 atacaaaaag ggccgcaggc tgttacccct gcggccggtt tcgggcgcat attgccatca
    12481 cggcagcctg acgcccgttt tcgccttact tccggttacg ccaccagccg acaatcgctg
    12541 cggtaataat tcccgccagg atcggtgctg ccaggtcgtg ccagaaaatc acggtaaact
    12601 gcgcgagcgt catatagccg ccttgttgta atgacaacat tttgcggcta ttcttgaatt
    12661 gttctggttc aagattagcc cccgttctgt tgtcaggtta tacctctcaa cgtgcggggg
    12721 ttttctcttt ccagcaacca atgccaccag ggataaagcc cccgcaacat tgcgcctcac
    12781 cggataacgc cggcttggtg tggatactac gactcaattc atcttcactt catccctgaa
    12841 atgtttgcaa tgaagagtgc attccggttt ttcaacagct gttacagtcg tttcgtgcag
    12901 gttgtggttc aggctcgcaa ttctgattaa caacctgtcc aggtagtatg aagaaataca
    12961 ataagcagga atttatatta tctcccccga tttccgtcat cggaggagat ataactgtca
    13021 ggcaaatatc gtcatcaaat aggcggcaaa cagtgccaga tgcgctgcgc cattgagcac
    13081 gttagtacgt ccggtggaga aggagatatg acacagcact aaagaggcca ccatcaccac
    13141 catttctggc gcaccaagtg caaactgcaa ttcgttaccc gtcataaagg caattagcgt
    13201 gacgacaggt acggtaagcg aaatggttgc taacactgaa ccaaagaaca gattcatcgc
    13261 gcgctggacc tggttgttca acactgcttt taacgcacct aaaccttccg gcgacagaat
    13321 caacagagcc accaggaagc cagtaaaggc gacaggggca ttcatgctgt cgagcaatgt
    13381 ctccagcggg ctggcgttca ttttggtcac cgcaataacg gcaatcagat ggacaatcaa
    13441 ccagatagca tgccacaggc tgctatgggc agacggttta ccgtgatgcg ggtcgtcgtc
    13501 atcactgtca tcttcgtgct cgtagacaaa caaactttga tgcgttttgg tctggatcag
    13561 caaaaatacg ccatacattg ccgcagaaat taatgctacc agtaacgcct gaccggttga
    13621 aaaattcgcc gcaggcagag ccattggaaa taccagtacg attatcgcca gggggaacag
    13681 cgcaattaaa tactgcttga taccaaacag attcatatat tgggtggcaa acttgcgtcc
    13741 gcccaacaat aatgaaaagc caaccagccc accggtaaca atcataatga ttgaatagag
    13801 cgtatcacgc attagcgttg gcgcggcgtc gccggttgcc attaaagctg aaatcaaact
    13861 gacttcaaga ataaccactg aaaggctaag aataagcgaa ccgtaaggct ctcccaggcg
    13921 atgggctaat acgtccgcat gacggacaac actaaaggcg ctacttaaaa taccaataag
    13981 agcaagaaga ttgatggcaa tgaccactgg tagtgtctgg ctgcttcccc acaggaacag
    14041 cactaccagc gccagaaccg ggaaaataag cgaagtctcc ttgtggcggg tttttaccgc
    14101 ctcttgagca tttgacatta tggttatccc tttgcagatg aatttatcga aaatgtaaaa
    14161 aataggtagg aaaaataaca gaaagtgtct ggatatcggt aacattttac gaatttttac
    14221 cccgctgtcg attttttact catttgggca taaaaataag taatacgttt agacaatgtt
    14281 tgttttagca cttcttaaga agagtctgac atgaaaattc ttatgttttg gcaagagtag
    14341 atattgttga ccacacttaa tgttcaactt tgtaaaagga gtcaacgatg ccgtataaaa
    14401 cgaaaagcga tctgccggaa agcgtaaagc acgtgcttcc gtctcatgcc caggatatct
    14461 ataaagaagc gttcaacagc gcatgggatc aatataaaga taaagaagat cggcgtgatg
    14521 acgccagtcg cgaagaaaca gcgcataaag tggcctgggc tgctgtgaag catgaatatg
    14581 ccaaagggga tgatgataaa tggcataaaa aatcgtaaaa ccggtagctt agttaaagct
    14641 attcgtgcgg tgttgccttg caagtggtcc gtggattgca tattgtcccg ttagtggttt
    14701 caaaatgagc agtaaaaatg tccggaagac accaaaaagt tgtcgcaggg aagtatgcag
    14761 tggcggaggt gtaaggtgat aacgcgtgat ttcttgatga atgccgattg taaaacggca
    14821 tttggtgcca ttgaagaatc actcttatgg tcagcagaac aacgggcggc ttcgctggcg
    14881 gcgacgctgg cttgtcgacc tgatgaggga ccggtgtgga tcttcggtta tggatcgttg
    14941 atgtggaatc cggcactgga gtttaccgag tcgtgcaccg gtacactggt tggatggcat
    15001 cgtgcgtttt gcctgcgcct gaccgccggg cgcgggactg cgcaccagcc gggacggatg
    15061 cttgcactga aagagggcgg acgcaccaca ggcgtcgcct atcgactgcc agaagagacg
    15121 ctggagcagg aactaaccct gttgtggaag cgcgagatga ttaccggctg ttatctgcca
    15181 acctggtgtc agcttgatct tgatgatgga cgcacagtaa acgccattgt gtttattatg
    15241 gacccgcgac atccagaata tgaatctgac actcgcgctc aggtcatcgc gccgttgatt
    15301 gcggcggcga gcggtccact gggaaccaac gcacaatacc tgttttcact ggaacaggag
    15361 cttatcaaac tgggaatgca ggacgatggg ctgaatgatt tgctggtatc ggtaaaaaaa
    15421 ctgctggcgg agaattatcc ggatggtgtg ttacggccgg gactcgcctg agtaaacttc
    15481 cctcatagtg gggcgtcaga cgccccctca aacattaaaa tgtgagcact ttatcggctg
    15541 acagcgtcca ttgcgccagt tccacaagcg taccgatttc caccccatca atcagaggaa
    15601 gtgtactaat cccgcgcccg tcggtacagg ttttgcacaa tttcaccggt acattctgag
    15661 cggtaaggat ctccagcatt tgctgaatgt tgtagccttc ccctggtttt tgcccgcgca
    15721 acccggctgt gaccgcatca gacatcagga acagacgcag atccagattg ctctcctgct
    15781 ctcgtaacgc aatggccagc cgcaagctgt taaataagga ttcgctcccg taaggtgcgc
    15841 cattggcaac gatcacgatt ttttgcatta tttactcctg tattcaggga attagacact
    15901 catcttctat cttactgctt ctgcagcgtc tgaccaatcg gtcacatttt taaggatttt
    15961 cctgaaagcg cgagaaaata cgacaaaagt tgccagtaat cgttattctt tagggctatg
    16021 gtttttcatt ttttaccgga agttaccgac gttttgagcc gtttcgttcc tcgcattatt
    16081 ccgttttatt tactcttgct ggcggcaggc ggtacagcta acgcacaatc taccttcgag
    16141 caaaaagcgg caaatccctt tgataataac aatgatggtc tgccggattt aggcatggca
    16201 cctgaaaatc atgatgggga aaaacacttt gctgaaattg tgaaagattt cggcgaaacc
    16261 agtatgaatg ataacgggct ggatactggc gagcaggcaa aagctttcgc attgggaaaa
    16321 gtccgcgacg cgtttagtca acaggttaat cagcacgtag agtcgtggct atcagcgtgg
    16381 ggaaatgcca gtgttgacgt caaagtggat aacgaaggac atttcaccgg cagtcgtgga
    16441 agctggtttg tgccgttaca agataatgat cgttatctca cctggagcca gcttggtctt
    16501 actcagcagg atgatgggct ggtgagcaat gtgggcgttg ggcaacgctg ggcgcgcggc
    16561 agctggctgg tgggttataa cactttttat gacaacttgc tggacgaaaa tcttcagcga
    16621 gcgggcttcg gtgccgaagc gtggggcgaa tatttgcgac tatcggcaaa cttttatcag
    16681 ccgtttgctg catggcatga acagacagcc acgcaggaac aacggatggc gcgcgggtac
    16741 gacctgacag cccggatgcg catgccgttt tatcaacacc tcaataccag tgtcagcgta
    16801 gaacagtatt ttggtgatcg tgttgatttg tttaactctg gtacgggtta tcacaatccc
    16861 gtcgcgttga gtctgggatt aaattatacc ccagtgccat tagtcactgt gacggcccag
    16921 cataaacagg gtgaaagtgg cgaaaatcaa aataacctcg ggctgaatct taattaccgc
    16981 tttggtgtac cgctcaaaaa acaactttct gcgggcgagg ttgccgaaag tcagtcgtta
    17041 cgtggtagtc gctatgacaa tccgcagcga aataatctac cgactcttga gtaccgacag
    17101 cgaaaaacgt taacggtgtt tctggcgaca ccgccgtggg atctaaaacc tggcgaaaca
    17161 gtgccgctga aattacaaat ccgcagtcgt tacggtattc ggcaactgat ttggcagggc
    17221 gatacgcaga tattaagttt gacgccgggc gcacaagcca acagtgagga gggctggacg
    17281 ctgatcatgc ctgactggca aaacggggaa ggcgcaagca atcactggcg attgtcagtg
    17341 gtggtggaag ataaccaggg gcagcgtgtc tcctccaatg agatcacgct aacgcttgtc
    17401 gaaccgttcg acgcattgtc aaacgacgaa ctgcgctggg aaccgtaatc agaaaatgcg
    17461 ctcctgatgc acccataccg cagcttccac gcgagacttt agcttcattt tcttcagcat
    17521 gtgcttgacg tgaactttta ctgtgctttc ggtgatgtcc aggcggcggg caatcatctt
    17581 gttcggcaaa ccctgggcaa ttagcttgag aatatcgcgc tcgcgtgggg ttaactggtt
    17641 aacatcgcgc tcagtagtgg cacggttagc gcgcaagctg gcggccagaa caggcgttaa
    17701 tgcttcgctt aataccattt cgccagcagc tgcctgatgt aacgctttca gcagatcttc
    17761 cggttccata tcttttaaca gatagccatc cgcgccgcgt ttcagtgcgg tgaccacatc
    17821 ttcttcatgg ttagagacgc tgaataccac aatgcgcccg gagagggact tttcgcgcag
    17881 tttatccagc gtttccagac cgttcatgcc gggcatattg agatctaaca ggatcagatc
    17941 gggatcaaga gactccgcca gttcaatacc ctgttcgcca ttactcgctt cgccaaccac
    18001 ggtgatatct ggtgccatac tgataagctg ttttacgcca gttcgcagca tcgggtgatc
    18061 gtcaatcagc aggatagtag ccggttcctg attactcatg ggtatctcct tggacgtctg
    18121 tgaaagtttt ttcgggaata aaggtgacca ccacttcggt gccacctgat tcacgacggc
    18181 ggacgcggca atcgccccgt aaactttgcg cacggtcgcg cattattatc atgccgtagt
    18241 gattgctgcg gatggcattt tcaggcacgc cgcagccgtt atcctggacg gtcagtttga
    18301 cctgattatc gttttgcgcc actgtcacca cgacctcact cgcttgcgaa tgtttgaggg
    18361 cgttacttaa tgcctcacgg gcaatttgca acagatggat tgcctgatgc gaaggcacca
    18421 gacgaggcgg caattgataa tccagcttca ccgggaagcc aaacctggcg ctgtactctt
    18481 cgcaactcgc ctccagcgcc ggacgtaatc caggctcggt gagctgcaag cggaatgtgg
    18541 tgagcaattc acgcaactgc gcccaggatg cattcagttc gttacggatc tgacttaaca
    18601 gttcgcggct gctttctggc agcgcatcgc cctgcatctg taaacaactc acctgcatct
    18661 tcatgcaaga gagagattgg gcaatagaat catgcagttc gcgcgcaatg gtggcacgct
    18721 cttccatcac gatcaactgt tgctgacgtt cctgatggcg atccagcgca agcgtggcgg
    18781 tgagttgttc aaccagggta tccaccagtt gttgttgatc atgactaaga tgacgcccct
    18841 gcggcagggt tgccagcaaa ataccgtact gcgtatgaga gtcagccagc cgccacttca
    18901 gggttgtgcc gcgatcgcca acggggagta caccgcgcgg gcagagctga cagcctttat
    18961 catcgcaggt catatctggc tggcaggtaa actcctgatg attctcttca tcatcagtgt
    19021 catacacccg cagttcgata tcacgtagca gggttaaatt ctgtaagccg ttgagtacag
    19081 gtgacaggcg ttcacacagc ggggcgcggg aatgcaaacg gcggttggcc tgccataaaa
    19141 aagagaggat ctgattttta tgttccagcc cggcggtttt ctcctgaacc cgctgctcaa
    19201 gtacggcgta actttccgcc agttctgcag acatattgtt caacgcagtt ccaagcatag
    19261 ccatttcgtt gcgcccgctg atgttcgcgc gttgggtaaa atcgcgatgg ctgacggcac
    19321 tcgccattgc caacaactgc cgccacggtt gtagcagtcg cgcccgcaac cagataatag
    19381 tgaacaccag taaaagtgcc ataaataccg ccattacccg atggaccagc accactgtct
    19441 caatgcgcat ttccgtggtg cggtcaaaac cagataccag ttgatcaagc ccggcaacaa
    19501 actggctgac atccgctgac accgtttcgc ggttttgtgc acgcattagc gcagggatca
    19561 gttcattacg ccagtaatct tgtaaaccct gtaattgcgc cagttgtccg tctcgttctg
    19621 ctgctcgggt taactcggcg ctaaatgctg tttgttccat ctctttaatt aagggcttgt
    19681 ctttctcgct taatggcact gctgccaaca gacggtaact ttgcatgcgc agcgatcccg
    19741 ctttgttgat cgcatgggcg ctgccctgaa cgccttgcac cagccagcca gaaactgcca
    19801 tccctgccag tccaatagca gtagaaagca acacaataag cgcaacctga ttaaccaggg
    19861 tgagcggaga gagacaacgt ttaagcatgc aaacctcttc cttcaggctt taaatgagca
    19921 ataaccttaa tgaatgtgac gatacattct ggaatggcag tattctcggc tattggctga
    19981 agtataccca tacccggaaa gagttactcc ttatttgccg cgtggttagt cgctttacat
    20041 cggtaagggt agggatttta cagcaccgtg aaaaatctca taatttttat gaaatcactg
    20101 tactcactat gggtaatgat aaatatcaat gatagataaa gttatcttat cgtttgattt
    20161 acatcaaatt gcctttagct acagacacta aggtggcaga catcgaaacg agtatcagag
    20221 gtgtctatga gtcactcatc cgcccccgaa agggctactg gagctgtcat tacagattgg
    20281 cgaccggaag atcctgcgtt ctggcaacaa cgcggtcaac gtattgccag ccgcaacctg
    20341 tggatttccg ttccctgtct gctgctggcg ttttgcgtat ggatgttgtt cagcgctgtt
    20401 gcggtgaact taccgaaagt cggttttaat tttacgaccg atcagctatt tatgttgacg
    20461 gcgctgcctt cggtttctgg cgcgttatta cgtgttccat actcctttat ggttcctatc
    20521 ttcggtggtc gtcgctggac ggcgttcagc actggtattc tgattattcc ttgcgtctgg
    20581 ctgggttttg ccgtgcagga tacctccacg ccttatagcg tcttcatcat catctctctg
    20641 ctgtgcggct ttgctggcgc gaacttcgca tccagtatgg caaacatcag cttcttcttt
    20701 ccgaaacaga agcagggtgg cgcgctgggt ctgaatggtg gtctgggcaa catgggcgtc
    20761 agcgtcatgc agttggttgc tccgctggtg gtatcactgt cgattttcgc agtatttggt
    20821 agccagggtg tcaaacagcc ggatgggact gagctgtatc tggcgaatgc gtcctgggta
    20881 tgggtgccgt tccttgccat cttcaccatt gcggcgtggt ttggcatgaa cgatcttgct
    20941 acctcgaaag cctccatcaa ggagcagttg ccggtactca aacggggtca tctgtggatt
    21001 atgagcctgc tgtatctggc aaccttcggc tccttcatcg gcttctccgc gggctttgcg
    21061 atgctgtcaa aaacgcagtt cccggatgtt cagattctgc aatacgcttt cttcgggccg
    21121 tttattggtg cgctggcgcg ttctgcaggt ggtgcattat ctgaccgtct gggcggaact
    21181 cgtgtcacac tggtgaactt tatcctgatg gcgattttca gcggcctgct gttcctgacc
    21241 ttaccgactg acggacaggg cggaagcttc atggcgttct tcgcagtctt cctggcgctg
    21301 ttcctgacag ctgggctggg tagtggttcc acttttcaga tgatttccgt gatcttccgt
    21361 aaactgacaa tggatcgcgt gaaagcagaa gggggctctg acgaacgtgc gatgcgtgaa
    21421 gcggcaaccg acacggcggc ggcgctgggt ttcatctctg cgattggcgc gattggtggc
    21481 ttctttatcc cgaaagcgtt cggtagctcg ctggcattaa cgggttcgcc agtcggcgca
    21541 atgaaagtat ttttgatttt ctatatcgcc tgcgtagtga ttacctgggc ggtatatggt
    21601 cggcattcta aaaaataaat cttaatattt gattatcctt tgcgcggcat gatgtcgcgc
    21661 tttttttacc tgtcatttag ttacaacatc cttatgaagt gtggcgtatt tcgccggttt
    21721 tcattaagag ccattaatat gttacccatg gggaatactc cttaataccc atctgcataa
    21781 aaatctcaat agtctaaata actatggatg caaaatatct taatttacag tgatttatgt
    21841 tgaatttatt aattatcctt aaggggtatc ttaggaattt actttatttt tcatccccat
    21901 cactcttgat cgttatcaat tcccacgctg tttcagagcg ttaccttgcc cttaaacatt
    21961 agcaatgtcg atttatcaga gagccgacag gctcccacag gagaaaaccg atgagtaaat
    22021 tcctggaccg gtttcgctac ttcaaacaga agggcgaaac ctttgccgat gggcatggcc
    22081 agcttctcaa taccaaccgt gactgggagg atggatatcg ccagcgttgg cagcatgaca
    22141 aaatcgtccg ctctacccat ggggtaaact gcaccggctc ctgcagctgg aaaatctacg
    22201 ttaaaaacgg tctggtcacc tgggaaaccc agcagactga ctatccgcgt actcgcccgg
    22261 atctgccaaa ccatgaacct cgcggctgcc cgcgcggtgc cagctactcc tggtatcttt
    22321 acagcgccaa ccgtctgaaa tacccgatga tgcgcaaacg cctgatgaaa atgtggcgtg
    22381 aagcgaaggc gctgcatagc gatccggttg aggcatgggc ttctatcatt gaagacgccg
    22441 ataaagcgaa aagctttaag caggcgcgtg gacgcggtgg ttttgttcgt tcttcctggc
    22501 aggaagtgaa cgaactgatc gccgcatcta acgtttacac catcaaaaac tacggcccgg
    22561 accgtgtcgc tggcttctcg ccaattccgg caatgtcgat ggtttcttac gcgtcgggtg
    22621 cgcgttatct ctcgctgctt ggcggtactt gcttaagctt ctacgactgg tactgcgatc
    22681 tgcctcctgc gtctccacaa acctggggcg agcaaactga cgtaccggaa tctgctgact
    22741 ggtacaactc cagctacatc atcgcctggg ggtcaaacgt gccgcagacg cgtactccgg
    22801 atgctcactt ctttactgaa gtgcgttaca aaggcaccaa aaccgttgcc gtcacgccgg
    22861 actacgctga aatcgccaaa ctgtgcgatc tgtggctggc accgaagcag ggcaccgacg
    22921 cagcaatggc gctggcgatg ggtcacgtga tgctgcgtga attccacctc gacaacccaa
    22981 gccagtattt caccgactat gtgcgtcgtt ataccgacat gccaatgctg gtgatgctgg
    23041 aagagcgcga cggttactac gctgcgggtc gtatgctgcg cgctgctgat ctggtggatg
    23101 cgctgggcca ggaaaacaat ccggaatgga aaactgtcgc ctttaatacc aatggagaaa
    23161 tggttgcgcc gaacggttct attggcttcc gctggggcga gaagggcaaa tggaatcttg
    23221 agcagcgcga cggcaaaact ggcgaagaaa ccgagctgca actgagcctg atgggtagcc
    23281 aggatgagat cgctgaagtg ggcttcccat actttggtgg cgacggcacg gaacacttca
    23341 acaaagtgga actggaaaac gtgctgctgc acaagctgcc ggtgaaacgc ctgcaactgg
    23401 ctgatggcag cactgctctg gtgaccaccg tttatgatct gacgttggca aactacggtc
    23461 tggaacgtgg cctgaacgac gttaactgcg caaccagcta tgacgacgtg aaagcctaca
    23521 cccctgcatg ggccgagcag atcactggcg tttctcgcag ccagattatc cgcattgccc
    23581 gtgaatttgc cgataacgct gacaagacgc acggtcgttc gatgattatc gtcggtgcgg
    23641 ggctgaacca ctggtatcac ctcgatatga actatcgtgg tctgatcaac atgctgattt
    23701 tctgcggctg tgtaggtcag agcggtggtg gctgggcgca ctatgtaggt caggaaaaac
    23761 tgcgtccgca aaccggctgg cagccgctgg cgtttgctct tgactggcag cgtccggcgc
    23821 gtcacatgaa cagcacctct tatttctata accactccag ccagtggcgt tatgaaaccg
    23881 tgaccgcaga agagttgttg tcgccgatgg cggataaatc ccgttatacc ggacatctga
    23941 tcgactttaa cgtccgtgca gaacgtatgg gctggctgcc gtctgcgccg cagttaggca
    24001 ctaacccgct gactatcgct cgcgaagcag aaaaagccgg gatgaatccg gtggactata
    24061 cggtgaaatc cctgaaagag ggttccatcc gttttgcggc agaacaacca gaaaacggta
    24121 aaaaccaccc gcgtaacctg ttcatctggc gttctaacct gctcggttct tccggtaaag
    24181 gtcatgagtt tatgctcaag tacctgctgg ggacggagca cggtatccag ggtaaagacc
    24241 tgggtcagca gggcggcgtg aagccggaag aagtggactg gcaggacaac ggtctggaag
    24301 gcaagttgga tctggtggtg acgctggact tccgtctgtc gagcacctgt ctctattccg
    24361 acatcatttt gccgacggcg acctggtacg aaaaagacga catgaatact tcggatatgc
    24421 atccgtttat tcacccgctg tctgctgcgg tcgatccggc ctgggaagcg aaaagcgact
    24481 gggaaatcta caaagccatc gcgaagaaat tctccgaagt gtgcgttggt catctgggca
    24541 aagaaaccga catcgtcacg ctgcctatcc agcacgactc tgccgctgaa ctggcgcaag
    24601 cgctggatgt gaaagactgg aaaaaaggcg aatgtgacct gatcccaggt aaaaccgcac
    24661 cgcacattat ggttgtagag cgcgattatc cggcaactta cgaacgcttt acctctatcg
    24721 gcccgctgat ggagaaaatc ggtaacggcg gtaaagggat tgcctggaac acccagagcg
    24781 agatggatct gctgcgtaag ctcaactaca ccaaagcgga aggtccggcg aaaggccagc
    24841 cgatgctcaa caccgcaatt gatgcagcag agatgatcct gacgctggca ccagaaacca
    24901 acggccaggt ggccgtgaaa gcctgggcgg cattgagtga gtttaccggt cgtgaccata
    24961 cgcatctggc gctgaataaa gaagacgaga agatccgctt ccgtgacatt caggcgcagc
    25021 cgcgcaaaat catctccagc ccgacctggt ctggccttga agatgaacat gtttcttaca
    25081 acgccggtta caccaacgtt cacgagctga tcccgtggcg tacgctctct ggccgtcagc
    25141 agctgtatca ggatcatcag tggatgcgtg atttcggtga aagcctgctg gtttatcgtc
    25201 cgccgatcga cacccgttcg gtgaaagaag tgatgggcca gaaatctaac ggtaatccgg
    25261 aaaaagcgct caacttcctg acaccgcacc agaagtgggg tatccactcc acctacagcg
    25321 acaacctgct gatgctgact ttaggtcgcg gtggtccggt ggtctggttg agtgaagccg
    25381 atgccaaaga gctgggtatc gccgacaacg actggattga agtcttcaac agcaacggtg
    25441 cattgactgc ccgtgcggtt gtcagccagc gtgttccggc agggatgacc atgatgtacc
    25501 acgcgcagga acgtatcgtt aacctgccgg gttcagaaat caccagccag cgcggtggta
    25561 ttcataactc ggtcacccgt atcacgccga aaccgacgca tatgatcggc ggctatgccc
    25621 atctggcata cggctttaac tactacggca ctgtaggttc taaccgcgat gaatttgttg
    25681 tagtgcgtaa gatgaagaac attgactggt tagatggcga aggcaatgac caggtacagg
    25741 agagcgtaaa atgaaaattc gttcacaagt cggcatggtg ctgaatctgg ataagtgcat
    25801 cggttgccac acctgttcag tgacctgtaa aaacgtctgg accagccgtg aaggcgtgga
    25861 atacgcgtgg ttcaacaacg tggaaaccaa gccgggccag ggcttcccga cagactggga
    25921 aaaccaggaa aaatacaaag gcggctggat ccgtaaaatc aacggcaaac tgcaaccgcg
    25981 catgggtaac cgcgccatgc tgctgggtaa aattttcgct aacccgcatc tgccggggat
    26041 cgacgattat tacgagccgt tcgattttga ctatcagaac ctgcataccg cgccggaagg
    26101 cagcaaatcg cagccgattg cccgtccacg ttcgctgatt accggcgaac ggatggcgaa
    26161 aatcgaaaaa gggccaaact gggaagatga cctgggcggc gagtttgaca aactggcgaa
    26221 agacaagaac ttcgacaaca tccagaaggc gatgtatagc cagttcgaaa acaccttcat
    26281 gatgtatttg ccacgcctgt gcgaacactg cctgaacccg gcatgtgtgg cgacctgccc
    26341 gagcggtgcg atttacaagc gtgaagaaga tggcatcgtc ctgatcgacc aggacaaatg
    26401 ccgtggctgg cgtatgtgca tcaccggatg cccgtacaaa aaaatctact tcaactggaa
    26461 gagcggtaag tctgagaagt gcatcttctg ctatccgcgt attgaagccg gtcagccgac
    26521 ggtgtgctct gaaacctgtg tcggtcgtat ccgttatctt ggcgtgctgc tgtacgatgc
    26581 cgacgcgatt gaacgtgcag ccagcaccga gaacgagaaa gatctctacc agcgtcagct
    26641 ggacgtgttc ctcgatccga acgatccgaa ggtcatcgag caggcgatta aagacggtat
    26701 tccgctgagc gttattgaag ccgcacagca gtcgccggtt tataaaatgg cgatggaatg
    26761 gaaactggcg ctgccgctgc atccggaata tcgcacgctg ccgatggtct ggtatgtgcc
    26821 acctctgtcg ccgattcagt ctgcggcaga tgcgggtgag ctgggcagca atggcattct
    26881 gccagacgtc gaaagcctgc gtattccggt acagtatctg gcgaatctgc tgaccgcagg
    26941 tgataccaaa ccggtactgc gcgcgctgaa acgtatgctg gcgatgcgtc attataaacg
    27001 tgctgaaacc gttgacggta aagttgatac ccgtgcgctg gaagaggtcg gtctgaccga
    27061 agcccaggca caggagatgt accgttatct ggcgattgct aactacgaag atcgctttgt
    27121 ggtgccgagc agtcatcgtg aactggcgcg ggaagccttc ccggagaaaa atggctgcgg
    27181 ctttaccttt ggtgatggct gccacggctc agataccaaa ttcaatctgt ttaacagccg
    27241 tcgtatcgat gccatcgacg tgaccagtaa aacggagccg cacccatgat cgaactcgtg
    27301 attgtatcgc gtctccttga atatccggat gctgccttat ggcagcatca acaagagatg
    27361 tttgaggcga ttgccgcgtc gaaaaatctg ccaaaagagg atgcccatgc gctgggcatt
    27421 ttcctgcgcg atttaacgac gatggatccg ctcgatgccc aggcgcagta cagcgaactg
    27481 ttcgaccgtg gccgcgccac gtcactgttg ctatttgaac atgttcacgg tgaatcccgt
    27541 gaccgcggcc aggcgatggt ggatctgctg gcgcagtacg agcagcacgg cttgcagtta
    27601 aacagccgcg aattgcccga ccatctgccg ctgtatctgg agtacctggc gcagctgccg
    27661 caaagcgaag ccgtggaggg tttgaaagat attgcgccga ttctggcatt gctgagcgcg
    27721 cgtctgcaac agcgtgaaag ccgttatgcc gtgctgtttg atctgctgct gaaactggcg
    27781 aataccgcca tcgacagcga caaagtggcg gaaaaaattg ccgatgaagc gcgcgacgat
    27841 acgccgcagg cgctggatgc agtctgggaa gaagagcagg ttaaattctt tgctgaaaaa
    27901 ggctgcggtg attcagcaat cactgctcat cagcgtcgct ttgccggtgc cgtcgcgccg
    27961 caatatctga atatcaccac cggaggacag cactaatgca attcctgaat atgttcttct
    28021 ttgatatcta cccgtacata gccggggcgg tcttcctgat tggtagctgg ctgcgttatg
    28081 actacgggca gtacacctgg cgcgcggcgt ccagccagat gctggatcgc aaagggatga
    28141 acctggcgtc gaacctgttc catatcggta ttctggggat ttttgttggt cacttcttcg
    28201 gtatgctgac gccgcactgg atgtatgaag cctggctgcc gattgaagtg aaacagaaaa
    28261 tggcgatgtt tgctggtggt gccagcggtg tgctgtgtct gattggcggc gtgctgttgc
    28321 tgaagcgtcg tctgttcagc ccacgcgtgc gtgcaaccac taccggagcg gatatcctga
    28381 tcctgtcgct gctcgttatc cagtgcgcgc tgggcctgtt gaccattccg ttctccgccc
    28441 aacatatgga cggtagcgag atgatgaaac tggttggctg ggcgcagtcg gtagtgacct
    28501 tccacggtgg cgcttctcaa caccttgatg gtgtggcgtt tatcttccgt ctgcacctgg
    28561 tgttggggat gacgttattc ctgctgttcc cgttctcgcg tctggtgcac atctggagcg
    28621 taccggtgga gtatctgaca cgtaagtatc agctggtgcg cgctcgtcac taagcgaatt
    28681 ttagtttgca tagaccccgc ttcggcgggg tttttttatg ggcacggtac ggggtgagtt
    28741 gtcggatgcg cttcgcttat ccgacctaca ggggaggata ttgtaggtcc ggtaaacgcg
    28801 gtgccattgg gcagaattag ctcggagtaa caggttttga gtatttacag gaagtgatgg
    28861 tggtggagga aggattactc agcgctgcgc gcttcgccct tcgggtcgtt gcctgcggcc
    28921 acgctctctc gctggcgctc gagtcgaacc ctggtcgaag cttctcatcc ttccccgctt
    28981 gggcagaata tttaatcgcg gattcgtttg agagttcacg gacttttgaa agtgatggtg
    29041 gtgggggaag gattcgaacc ttcgaagtcg atgacggcag atttacagtc tgctcccttt
    29101 ggccgctcgg gaaccccacc aggggtaatt caaattttga ggtaatgctt gagatggtgg
    29161 tgggggaagg attcgaacct tcgaagtcga tgacggcaga tttacagtct gctccctttg
    29221 gccgctcggg aaccccacca cggggtaatg ctttttacta gcctgctccc ttatcgggaa
    29281 gcggggcgca tcatatcaaa tgacgcgccg ctgtaaagtg ttacgttgag aaaaatgaac
    29341 tggttgcgta attttcaccc gtaacggatt aaaggtaacc agttattttt gctggcgatt
    29401 aaagaataat cgtccgatta ccgtaaacaa agacgcgctg cgccagtact ttgtagagtg
    29461 cgcgacttaa gacgtttttc tcgacgtcac gacctgcgcg catcatatct tcagctgtgt
    29521 aggtatgatc gacatgaata acgtcctgca tgatgattgg accttcgtcc agattgtcat
    29581 tcacatagtg agcggtagcg ccaataatct tcacgccacg ttcataggcc tggtgataag
    29641 gacgtgcgcc gataaacgct ggcaggaatg aatggtgaat attgatgatc ttattcggga
    29701 agcgtgccac aaattctggc gttaataccc gcatatactt cgccagcacc acgtagtcag
    29761 gctgataagc atcaatggca tccgccatct tttgatcgtg ctcgttgcgg cttaaccctt
    29821 catggcttac cagctcaaac ggaatatcaa aacgctcaac cagagaacgt aaagtatcgt
    29881 ggttaccaat aactgccgcg atttcgacat ccaggccgcc gtaattggct ttcatcaaca
    29941 aatcgccaag gcaatgcgcc tctttagtga ccagaatcac tatccggcga cgaccggcag
    30001 gattcagctc acgtacggag ccttctggca atgcgctgtc gagatccgcc agcagggtgg
    30061 aatcattaaa aattccttcc agttccgtgc gcataaaaaa gcgcccggta cggtgatcaa
    30121 caaattcatt gttctgtacg atatttaact cgtgcttgta gcaaatattg gtaatacgtg
    30181 cgatcagacc tttttggtcc ggacaaatag tacgcagaac tttacgttgg agtgaatgca
    30241 ttgctggaaa aaccttgttg agagtgtttg ctaaaccgta ccgtcaacca ttattggccg
    30301 cagcactttt taaatttttt acctgaacca caagggcagg gatcgttgcg gccaaactgc
    30361 ggacgtgtac cgtcaatata gtaccattga ccgttttctt tcaaaaatcg cgaacgttca
    30421 attattgcac ctgttttgct accttcggta aatcgcgcaa caaaactgac aaatccgata
    30481 ttgtcgacgt cctgccaaca atgttcaaat acggtcaatc ccaaccattc agtatgcgca
    30541 aatccggcaa tcaattcggc acgtaacgtc gccgctccac aagatggatg ccaggtcttt
    30601 attaaataat ctgcgtcttt catcacaaaa gcgcagtaac gcgaacgcat gagatgttct
    30661 ggatcaggtg caaccttttc accagacaca taagggtggc aacataggct atactcgaca
    30721 gcactaccac agggacaaag ctgagacaca aataatctcc ctggaaacaa taacggcgta
    30781 ttaaccgcct gagtagcact atgttaaccg agcagtagcg atgttgctac gattgcattc
    30841 caggggaatc ttgcgggaat aatgagaaag ataaaaatag ggctggcgct gggatctggc
    30901 gcggcgagag gttggtcgca tattggcgtt attaatgcgc taaaaaaagt gggtattgaa
    30961 attgatatcg ttgcaggatg ttcaattggt tcgctggtgg gcgctgccta tgcatgcgat
    31021 cgactatctg cgctggaaga ttgggtgacc tctttcagtt attgggatgt tttacgcctg
    31081 atggatctct cctggcagcg cggtgggtta ctgcgcggcg agcgtgtctt caatcaatat
    31141 cgcgaaataa tgccggaaac agagatcgaa aattgttccc gtcgcttcgc ggctgttgcc
    31201 accaatttaa gtactgggcg cgaattatgg tttactgaag gcgatctcca tcttgctatt
    31261 cgtgcatcat gcagtattcc aggactaatg gcacccgttg cgcataacgg ctactggctg
    31321 gttgatggcg ctgtagttaa cccaattcct atttccctca cgcgtgcatt gggggctgat
    31381 attgtgatag cggtcgacct gcagcacgat gctcatttga tgcaacaaga tttgctctcc
    31441 tttaatgtca gtgaagaaaa tagcgagaat ggtgattctc tgccgtggca tgcgcgtctg
    31501 aaagaaaggt taggcagcat aacgacacgt cgggcggtga cagcgccaac ggcaacagag
    31561 attatgacca cttctatcca ggtgctggag aaccgcctta aaaggaaccg catggcaggt
    31621 gatccgcccg atattctgat tcaacctgtt tgcccgcaaa tatctacgct tgatttccat
    31681 cgcgcgcacg ctgccattgc ggccgggcag ctggcagtgg aaaagaaaat ggacgaactt
    31741 ttgccgttgg tacgcaccaa catttgacca gaatttttat ctacacttaa gttaattctg
    31801 acaggcgcag gtggcaatag catgccacta ttgagtaaag ccagtcaggg gagagaacat
    31861 gacgcagcca ttggtcggaa aacagattct cattgttgaa gatgagcagg tatttcgctc
    31921 gcttctggat tcgtggtttt cctcattggg agcgacaacg gtactggcgg ctgatggggt
    31981 ggatgccctt gagttgctgg gaggtttcac tccagacctg atgatatgtg atatcgcgat
    32041 gccacgaatg aacgggctta aactgctgga gcatatacgt aacagaggcg accagacccc
    32101 agttctggtg atatctgcca ctgaaaatat ggcagatatt gccaaagcgt tacgtctggg
    32161 cgttgaagat gttttgctga aaccagttaa agatctgaat cgcttgcgcg agatggtttt
    32221 tgcctgtctc tatcccagca tgtttaattc ccgcgttgag gaagaggaaa ggctttttcg
    32281 cgactgggat gcaatggttg ataaccctgc cgcagcggcg aaattattac aggaattgca
    32341 accgccggtt cagcaggtga tttcccattg ccgggttaat tatcgtcaat tggttgccgc
    32401 ggacaaaccc ggcctggtgc ttgatattgc cgcactttcg gaaaacgatc tggcatttta
    32461 ctgccttgat gtcacccgag ctggacataa tggcgtactt gctgccttgt tattacgcgc
    32521 attgtttaac ggattattac aggaacagct tgcacaccaa aatcaacgtt tgccagagtt
    32581 gggcgcgtta ttgaagcagg taaaccattt actccgtcag gccaatctgc cggggcagtt
    32641 tccgctatta gttggctatt atcatcgcga actgaaaaat ctcattctgg tttctgcggg
    32701 tctgaatgcg acgttaaata ccggcgaaca ccaggtgcaa atcagtaatg gtgttccgtt
    32761 aggcacttta ggtaacgctt atttgaatca attgagccag cgatgcgatg cctggcaatg
    32821 ccaaatatgg ggaaccggtg gtcggcttcg cttgatgttg tctgcagaat gagcaaacga
    32881 taacgcgggc taaatttgca ttacctgcta atgtcggctg gtggtactat cgtcgccatt
    32941 cgtataagta attgtcttaa ttatgctaac tcgcctcctt ttcagaactt agccccttcg
    33001 gggtgctgat atactgggat gcgatacaga aatatgaaca cgttcaaaac acgaacagtc
    33061 caggagaatt taaatggctg ccattaatac gaaagtcaaa aaagccgtta tccccgttgc
    33121 gggattagga accaggatgt tgccggcgac gaaagccatc ccgaaagaga tgctgccact
    33181 tgtcgataag ccattaattc aatacgtcgt gaatgaatgt attgcggctg gcattactga
    33241 aattgtgctg gttactcact catctaaaaa ctctattgaa aaccactttg ataccagttt
    33301 tgaactggaa gcaatgctgg aaaaacgtgt aaaacgtcaa ctgcttgatg aagtgcagtc
    33361 tatttgtccg ccgcacgtga ctattatgca agttcgtcag gggctggcga aaggcctggg
    33421 acatgcggta ttgtgtgctc acccggtagt gggtgatgaa cctgtggctg ttattttgcc
    33481 tgacgttatt ctggatgaat atgaatccga tttgtcacag gataacctgg cagagatgat
    33541 ccgccgcttt gatgaaacgg gtcatagcca gatcatggtt gaaccggttg ctgatgttac
    33601 cgcatatggc gttgtggatt gcaaaggcgt tgaattagcg ccgggtgaaa gcgtaccgat
    33661 ggttggtgtg gttgaaaaac caaaagcgga tgttgcgccg tctaatctcg ctattgtggg
    33721 tcgttacgta cttagcgcgg atatttggcc gttgctggca aaaacccctc cgggagctgg
    33781 tgatgaaatt cagctcaccg acgcaattga tatgctgatc gaaaaagaaa cggtggaagc
    33841 ctatcatatg aaagggaaga gccatgactg tggtaataaa ttaggttaca tgcaggcctt
    33901 cgttgaatac ggtattcgtc ataacaccct tggtacggaa tttaaagcct ggcttgaaga
    33961 agagatgggc attaagaagt aacatccgta tcggtgttat ccacgaaacg gcgttgagca
    34021 atcgacgccg tttttttata gcttattctt attaaattgt cttaaaccgg acaataaaaa
    34081 atcccgccga tggcgggatt tttaagcaag tgcaatctac aaaagattat tgcttgatca
    34141 ggaaatcgtc gagggattta ccttgctcat ccattgcttt tttgattaca gctggagtac
    34201 ggccctggcc agtccaggtt ttagtttcgc cgttttcgtc aacgtagcta tattttgccg
    34261 gacgctgagc acgtttagct ttggtgccag atttaacggc agcaaggcta ttcagcagtt
    34321 cgttcgggtc aataccgtca gcgatcagca tttcgcgata ttgctgcagt ttacgagtgc
    34381 gctcttcaac ttcagcagca gccgcgcttt cttcttcgcg acgttcgtta acaacaactt
    34441 ctaatttttc cagcatttct tccagcgttt caagtgtaca ttctcttgcc tgcgcacgaa
    34501 gagtacggat gttgttcaga attttaagtg cttcgctcat tgtagtaatc tcaaacttat
    34561 attggggtgg tttgttgagg taataataga gccttaaatt cagttgtgca atagccagga
    34621 atgtaaggaa ttcaaaattg ttctttattt tgtgccgcca ataaatatct tttcataaaa
    34681 ttagccagaa aagacgcggc atatagccct atttgcgccg atgatttcgc agcacgtgag
    34741 gttaaaactt cctgattcat gtcacatttt atggggagat tatcgtaggc tgacgacctt
    34801 tcagtcttct gtattagtcg tgtttacaag aattccctat taagcgaatg atgaaaagta
    34861 gaacagtcgc aataagagca gggacttagt attgcactat ctcctggagg tcaacagagg
    34921 gctattactt gcgcaacagg ttaaagattg tgaatagtta ccagcagtca tttacccgct
    34981 tataacaagc gaggcagttg taatgatagc tcagaaggat tatgcaaggc ttcgtaaggg
    35041 agaacgcata taccacttct gtgcatactg ttgagctgaa aaactgacga attatgataa
    35101 actccagcca actttaattc atatcattga gggcctgtgg ctgatggcac agctatattt
    35161 ctactattcc gcaatgaatg cgggtaagtc tacagcattg ttgcaatctt catacaatta
    35221 ccaggaacgc ggcatgcgca ctgtcgtata tacggcagaa attgatgatc gctttggtgc
    35281 cgggaaagtc agctcgcgta taggtttgtc atcgcctgca aaattattta accaaaattc
    35341 atcattattt gatgagattc gtgcggaaca tgaacagcag gcaattcatt gcgtactggt
    35401 tgatgaatgt cagtttttaa ccagacaaca agtatatgaa ttatcggagg ttgtcgatca
    35461 actcgatata cccgtacttt gttatggttt acgtaccgat tttcgaggtg aattatttat
    35521 tggcagccaa tacttactgg catggtccga caaactggtt gaattaaaaa ccatctgttt
    35581 ttgtggccgc aaagcaagca tggtgctgcg tcttgatcaa gcaggcagac cttataacga
    35641 aggtgagcag gtggttattg gtggtaatga acgctacgtt tctgtatgcc gtaaacacta
    35701 taaagaagcg ttagaagtcg gctcattaac ggctattcag gaaaggcatc gccacgatta
    35761 ataagaattt ctttactgac agggtgagca gggcgctttt atcctgtcag ttcgttttac
    35821 tcactttatc cggtgtatat acccttctcg acagttcttt aacgccgcta tacccctcac
    35881 agagcgcttt tacagtgacg ttgacttgtg acctgtaaag tacaatatcg ctgtgtgtag
    35941 gcgttataca tcgccgtaaa tatgatgcag gcaaatgctg tcggtttatg ggaaagttgc
    36001 tttgggtaaa aaaaacggcc ccagaaggga taatgccagt cagttaagca actgactggc
    36061 tctttttcgg ggctgtgggg tatttccagg gcctctcctt taccactctc gggaaggc
//
LOCUS       DAZGHF010000035        36009 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGHF010000035 DAZGHF010000000
VERSION     DAZGHF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.035"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..174)
                     /locus_tag="ACHK3A_001850"
     CDS             complement(<1..174)
                     /locus_tag="ACHK3A_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739934.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALN"
     gene            complement(270..893)
                     /locus_tag="ACHK3A_001851"
     CDS             complement(270..893)
                     /locus_tag="ACHK3A_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8739935.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRMVGRENTSSPYQQIAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTSVEVSDNLTGTTRKIPAYHL"
     gene            complement(982..1491)
                     /locus_tag="ACHK3A_001852"
     CDS             complement(982..1491)
                     /locus_tag="ACHK3A_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739936.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGVCGQ
                     QIQTFCSSFHRRVHERITERDHTVSPSAPVVLRWLREQHEGAPIRCLLLLSQTSVCHP
                     RASVTVDEACSQVADLLQQTWPVISAGGQCRVERCFRVVRPDTSEQYVALKTAVQSLM
                     PRGIATIIR"
     gene            complement(1772..1950)
                     /locus_tag="ACHK3A_001853"
                     /pseudo
     CDS             complement(1772..1950)
                     /locus_tag="ACHK3A_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
     gene            complement(2018..2288)
                     /locus_tag="ACHK3A_001854"
                     /pseudo
     CDS             complement(2018..2288)
                     /locus_tag="ACHK3A_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2338..2904)
                     /locus_tag="ACHK3A_001855"
     CDS             complement(2338..2904)
                     /locus_tag="ACHK3A_001855"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO8739937.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGTIERHLGCSDF"
     gene            complement(3376..3801)
                     /locus_tag="ACHK3A_001856"
     CDS             complement(3376..3801)
                     /locus_tag="ACHK3A_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO8739938.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDSHALSAQQKATCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRATCTKSVYRYQPDLCQDCGGAST"
     gene            complement(3798..4181)
                     /locus_tag="ACHK3A_001857"
     CDS             complement(3798..4181)
                     /locus_tag="ACHK3A_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO8739939.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            complement(4552..5145)
                     /locus_tag="ACHK3A_001858"
     CDS             complement(4552..5145)
                     /locus_tag="ACHK3A_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO8739940.1"
                     /translation="MYNANPNYEMNFAILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRLDSTLSEICSLV"
     gene            complement(6062..7495)
                     /locus_tag="ACHK3A_001859"
     CDS             complement(6062..7495)
                     /locus_tag="ACHK3A_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO8739941.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQQNTQAPLAMVATSALTA
                     MSIACQNQIDVCRPGNLRGPVNIYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNNSPVPPQIRRTI
                     FNDTTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTMSAMIQPDVYHKGFCTRKKELVKTSGHHARFLMCQPTSMQGTRIITG
                     DNYSSQYQELFDKRINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYMRLSNPL
                     TFDNTALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            complement(7591..7818)
                     /locus_tag="ACHK3A_001860"
     CDS             complement(7591..7818)
                     /locus_tag="ACHK3A_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8739942.1"
                     /translation="MWVPLTDEQRSVILKAYGTEGDRLVREKERKEITSISRSQAWKL
                     EREGNYPPRKTIGKKSCGWLLSDLLWWIQTR"
     gene            8548..10161
                     /locus_tag="ACHK3A_001861"
     CDS             8548..10161
                     /locus_tag="ACHK3A_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739943.1"
                     /translation="MVNQTMQQNQYYRILSGESRPFPGIYEAVSEQLLLLIFKARQLY
                     QDPEYRPWFEEQHKVLLALRDNTELQKIFNKQRKENFALGASTSSQPTALTNALNVMC
                     HLYNGFDEGNISLKSWGAMQEVQTTPDFFSLGPRTWKTPELAKFVGDDLFNILTECKA
                     WSSPIPAEARQGYCHQDLSVAHSCTSELLAHLKQYHEIDESTILAITIGLAVNEFYQY
                     KQNIPEWLSCFENKNKWPWDCRKSDTEDVHRLRERLSIIGCTEFVKNMFSVASQHEIK
                     TMYNIYVHIYAAEICGWVKTSMAYFKPTDFIQYTWKFATVTPDKDEKTENTTAWERDY
                     CQITIQRDELLRLIRTNTLQDAAIHLSGCLQYAYLESVKNDIDYPGIAPNAEEKKMKY
                     TDSIEFSGIRNNIINAVKRLESFEKWYETNKVLLVSNKTIRTEKIDVVVRLAGLKAYD
                     LQEGVPDGLKWKVKDGINERIKTDTSLCLPQDISDTSLNRYRHAVTRIVNDEIDTLLM
                     EQKKKNKRFPYSKDRHAIQPLWSKCVIEQ"
     gene            complement(10254..10850)
                     /locus_tag="ACHK3A_001862"
     CDS             complement(10254..10850)
                     /locus_tag="ACHK3A_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8739944.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDIRHKVVATLELQRRSQLEGVFVRKFWEEQDVAQIEGIVVDGAVRDVGL
                     ATFVYETVATKAGVILLSDNEQYEGGKALWQHIARRSTNLKVFILDTDSARYYPFDGD
                     RICYDGESIPESEIWSEHPDRNKHGVVLVAESVNGKAA"
     gene            complement(10980..12296)
                     /locus_tag="ACHK3A_001863"
     CDS             complement(10980..12296)
                     /locus_tag="ACHK3A_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8739945.1"
                     /translation="MAVNLTETAIRALKAKKTSFYVWSNSSQRGTGRLGVKVQSSGSK
                     IFYFRYYVEKGTKERFIQLGIWPEMKLATANELAKKYGAWLIEGKDPQTELERQQLAE
                     QQRRQLHQSQGSFEALIHGYVNKMKIDNKRTWQDVLKRLENECYTVIPRETKAKDVTS
                     GQIKHILAGIIQRGAVVHSNRIRSYLMAAFNYGLKADNDPMNTSVGISFGLEANPVSV
                     IPKQSSAEKVGDTWLTLEELRFLMEHFAEATNVGLLMQHLIRLCIYTGGQRPFEMIAS
                     QWCDVDFQQKTLLVTADVSKNKREHLIPLTESALQELSCVQELTKEKSSPYIFPLSTN
                     GKRPVRTDSLARAIMYFRACNPDFKIFTARDLRRTCKTLMGEAGISKEIRDRIQNHAL
                     NDVSSKHYDRYDYLPEKRRALEIWEDRVNNYQQQTGNNVVNLFGRR"
     gene            complement(12593..13357)
                     /gene="nfeF"
                     /locus_tag="ACHK3A_001864"
                     /gene_synonym="yqjH"
     CDS             complement(12593..13357)
                     /gene="nfeF"
                     /locus_tag="ACHK3A_001864"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO8739946.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISVGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            13646..14269
                     /gene="nfeR"
                     /locus_tag="ACHK3A_001865"
                     /gene_synonym="yqjI"
     CDS             13646..14269
                     /gene="nfeR"
                     /locus_tag="ACHK3A_001865"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO8739947.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(14373..15893)
                     /gene="aer"
                     /locus_tag="ACHK3A_001866"
     CDS             complement(14373..15893)
                     /gene="aer"
                     /locus_tag="ACHK3A_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO8739948.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLTAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQDGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            16311..17690
                     /gene="ygjG"
                     /locus_tag="ACHK3A_001867"
     CDS             16311..17690
                     /gene="ygjG"
                     /locus_tag="ACHK3A_001867"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO8739949.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCEQVIKAARKALA
                     AMRVSVEEA"
     gene            complement(17732..18064)
                     /gene="ygjH"
                     /locus_tag="ACHK3A_001868"
     CDS             complement(17732..18064)
                     /gene="ygjH"
                     /locus_tag="ACHK3A_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO8739950.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            18283..19266
                     /gene="ebgR"
                     /locus_tag="ACHK3A_001869"
     CDS             18283..19266
                     /gene="ebgR"
                     /locus_tag="ACHK3A_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO8739951.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            19450..22542
                     /gene="ebgA"
                     /locus_tag="ACHK3A_001870"
     CDS             19450..22542
                     /gene="ebgA"
                     /locus_tag="ACHK3A_001870"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO8739952.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENNLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            22539..22988
                     /gene="ebgC"
                     /locus_tag="ACHK3A_001871"
     CDS             22539..22988
                     /gene="ebgC"
                     /locus_tag="ACHK3A_001871"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO8739953.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVIYDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            23051..24484
                     /gene="ygjI"
                     /locus_tag="ACHK3A_001872"
     CDS             23051..24484
                     /gene="ygjI"
                     /locus_tag="ACHK3A_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8739954.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            24618..25688
                     /gene="ygjJ"
                     /locus_tag="ACHK3A_001873"
     CDS             24618..25688
                     /gene="ygjJ"
                     /locus_tag="ACHK3A_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO8739955.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDTKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVSGSES
                     GMMVSAMYEALKGIYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            25705..28056
                     /gene="ygjK"
                     /locus_tag="ACHK3A_001874"
     CDS             25705..28056
                     /gene="ygjK"
                     /locus_tag="ACHK3A_001874"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO8739956.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSATLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNKRNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKAETLSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFT
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            28227..30245
                     /gene="fadH"
                     /locus_tag="ACHK3A_001875"
     CDS             28227..30245
                     /gene="fadH"
                     /locus_tag="ACHK3A_001875"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO8739957.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVGLAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KSHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQQLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(30290..30706)
                     /gene="higA"
                     /locus_tag="ACHK3A_001876"
     CDS             complement(30290..30706)
                     /gene="higA"
                     /locus_tag="ACHK3A_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO8739958.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(30703..30786)
                     /locus_tag="ACHK3A_001877"
                     /pseudo
     CDS             complement(30703..30786)
                     /locus_tag="ACHK3A_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            complement(30977..32113)
                     /gene="rlmG"
                     /locus_tag="ACHK3A_001878"
     CDS             complement(30977..32113)
                     /gene="rlmG"
                     /locus_tag="ACHK3A_001878"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO8739959.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            32198..32701
                     /gene="ygjP"
                     /locus_tag="ACHK3A_001879"
     CDS             32198..32701
                     /gene="ygjP"
                     /locus_tag="ACHK3A_001879"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO8739960.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            32778..33461
                     /gene="ygjQ"
                     /locus_tag="ACHK3A_001880"
     CDS             32778..33461
                     /gene="ygjQ"
                     /locus_tag="ACHK3A_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO8739961.1"
                     /translation="MQARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTSKQL
                     TWSDVSAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVGAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAQK"
     gene            33540..34526
                     /gene="ygjR"
                     /locus_tag="ACHK3A_001881"
     CDS             33540..34526
                     /gene="ygjR"
                     /locus_tag="ACHK3A_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO8739962.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKNVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            34809..35774
                     /gene="alx"
                     /locus_tag="ACHK3A_001882"
     CDS             34809..35774
                     /gene="alx"
                     /locus_tag="ACHK3A_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO8739963.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         9181 a         9134 c         8812 g         8882 t
ORIGIN      
        1 gttcagagca tcccaggaaa cgttcataca gaagctgtcc tgcaatttgc aggcaaccac
       61 ctctgccagg cggttccggt tatccgccag tccggcataa cctctctctg ccagccactc
      121 attcagttca tgttccagag acagattgat aatatcgtaa tcggaccttg ccattcccgt
      181 gtccttagca catgttttca ggcacgggga taaagtataa agaatgtcgt actgctgctg
      241 ccagagcaat attccccctg atggtcgtat cagagatggt atgccggtat tttgcgggtt
      301 gttcccgtca ggttatcgct cacttccacg gacgttgtca ccaccggcat tccggccttt
      361 ctcagcctca aaacatcagc tgcaatacgc tgactaccag accagaacag gccgtccagt
      421 gcagtcacca gtaaccccgc ctccagcgca tacttcagcc gttcacgggg cgctttcact
      481 tcccgggcaa tctgctggta tggcgatgat gtattttcac gcccaaccat ccggcgaata
      541 cgatgagaca gatgataccg gtatgtatcc ggcacaccgg aaaggctggc cttcaggctg
      601 tacacgcagc caaagcgttt atcgttgaac accacatttt tctggctgat atcccattct
      661 tcacgcagcg cggcaatcag ttgtggtgta cgggtaagca acaagcgaaa aggcagttca
      721 aaactggtga cgtaatccac attcagcagg gcaatgcgaa gtcgttcttc tggtccggct
      781 tctgtctgcc ggcactcctc caggacatcc tgccactgca ggcgaagacg ggaagactca
      841 ttcagttctg taaagcagta tttatccgcc agatagtcaa ttcgtgtatg catactgaag
      901 agtattccgt ataaagattc agctggcaaa actttatcag tctgtaaaaa ctaacggaag
      961 agtcgatatt tctcccgtcc atcaccggat gattgttgca ataccccgtg gcatcagaga
     1021 ctgaacagcc gtttttaacg caacgtattg ctctgatgta tcaggccgga caacccgaaa
     1081 acacctttcc acccggcatt gtccgccagc gcttatcacc ggccaggtct gttgcagtaa
     1141 atccgccact tgcgaacatg cttcatcaac tgtgacactg gcccgcggat ggcaaacact
     1201 cgtctggctg agcagcaaca gacatcgtat cggtgctcct tcatgttgtt cccgcaacca
     1261 gcgtaatacc accggcgctg atgggctgac cgtgtgatca cgctccgtaa tacgttcgtg
     1321 cacccgtcgg tgaaaggaac tacagaatgt ctgaatctgt tgaccacaga cgccttctgt
     1381 cgaatgaagt gtgaagtgga ttgccagcag atgcggccag tgatccaccg cctgctgaac
     1441 aaaacgccgg atttcccccg gctctgaaag taaggcttcg gttatttgca ctattttatc
     1501 tctgttgaac ttggttaagt cggtgcagac gcatcaacac aagtacggtt cgatgcaaac
     1561 agctgtgact ggcaatatga aaggaatgat gaatcagtca ggatgacaaa gtgccggctg
     1621 accggagggg acgcaggaag attcacgggg ggaccagcac cagggaacag cgccacaata
     1681 ccaacgctga cacgttgaac attgccagcg taccggtatc acaacacgtt tcatacttct
     1741 gccaccgtga ttcttcgatt cgttactgta tctactgtga cacttcgctt ttatacctgc
     1801 ggctggatcg gcccggcttg atgaatcttc actgatcagc ttataaaacc ctctgtcggt
     1861 cataccggtg aaactggtga tatagttcat gtcaatcagg gaattatcgg cacgcagaaa
     1921 tacgctgtcg tggcttgttg tagtcaacat ggtcagaatg tcctctgtga gatttatgaa
     1981 gattgtgcga atgcggggaa tctgctgagc tgtgctttca gaactggcct gttacgggat
     2041 accagggatt accggcgggg taacgggctt ccggatcata cacaccacga ttatcgcgga
     2101 caaaatcact gaacgcccat atcacctctt taagtatgtc ttcgcagtcc ggtacatgac
     2161 gatccagcgc cacatcccga gtggtactgc tttgatgcgc ccggtgacac aaagcccgga
     2221 ttgttccaga catcctgaat caaacgcccc agattagggg cgtcgaaata tgcctctctg
     2281 accattatat tccggcgtac aggtagcagg tcagaagtga caatgcgtca cctgacgtta
     2341 aaagtcacta cacccaagat gacgttcaat ggtaccatgt gattcaatgt aagcccgggc
     2401 cgtctgttcc agtacaccag gctcagcgtt gtatgtgtta gctgcatcaa ataccaacga
     2461 cagcacttca ggatacacaa ccagatgtgt aatggagtta tcttcaccca atacttttcc
     2521 ccacgcctgc tcaatcagat ttctgagaac caccacctca cgactcttac accagacatc
     2581 gttattaagt agcagcacca taagataagg agtggtatcg ttagccacag cttccctact
     2641 ccagagataa tataaagggg tgggctcaac agattgatct ttacgtcgct tacactgcaa
     2701 atattcagaa atgagtctat gcagttcacc agtaaaatca gccatcagag agggaatggc
     2761 cttattaata ccctgccctg gtattaattt aaattgtaat aatttaattt caggatgtgt
     2821 ggctgcagcc cgatacagag ttgcaaggac acacttttgc cagagggcgt tacaggaaag
     2881 cttaacattt gattctgtat acataataaa tcaccttaca gttacaacag gtcaaaaacc
     2941 gctgtagcca gagttacgct ggcctgatgc tttagtaccg ggcatcgtct gataatccag
     3001 acgctccaat aagcgctgat actgctcagg gaaatcagga tcatgaatat cctggatgtc
     3061 acgtccatta gcagggaaat aaacaatgca gccccctgga ttaactatgc agaaatcgtc
     3121 ctgaggcact gataaatacg gagaggactc tcgcgtatgg ttggttgaca ccacagtgcg
     3181 gattcggcga atccgcgatc acggtgcgat ttcgcaccac agtacacaat caataccccg
     3241 ggttttattc aggtaagcag gactgcggat atccggtgtc gcgcctttgt gtcacgaacg
     3301 gggtgggtgc gaaacaccag ataaaatgca ggttccgata cctctgaacg ccctgagcag
     3361 gtcgggcatt tcggattaag tactcgcacc tccgcagtcc tggcacaaat ctggctggta
     3421 cctgtaaaca gacttcgtac atgttgctct gcaataaatc cccgtaccac aggcctcaca
     3481 gaatttcttc ccatgaaaaa actgctcaca catcacacaa tgccactcca gcacgaccgg
     3541 taatggcgat agaaacatcg ccatatcctc aatgtaaggg tgggactttt ccggattcag
     3601 caccacgcag gtcgccttct gttgcgcgct cagggcatgt gaatcatgct caaaccacgc
     3661 cccctgagca tctgtctgca aaatcaaccg accacgacag gaaaggcaga aacactgccg
     3721 gatatttctg ctgagcgcag agtcggcact gataatgtgc tcacccggcg tgatccccag
     3781 ccccgttttt ataccgttca ttctgctccc ccctcctcac tgaagcgccc ggtctggcag
     3841 tgtgtgcagt actgctcccc ataataatcg tggtgacatt gcctgcagtg ccagctggct
     3901 ttacgtacta cgggtaaggc atccgggacg aattgctgca gacgtttaat cagttgtatc
     3961 tccctgcgtt ccggtctgac ataagggcac tgctgaccgt gctccgtcag cccgtcgtca
     4021 gtgtgttcaa accagggacg ctcagtgtcg tattgcgggt ggtatctgag cgcactgccg
     4081 caaaggtggc aagtgtagcg atcgtaaggt gcagtctgtg cggtacgggc acccgtcaga
     4141 cgcccgttgc catcaaatgc aataaaggat tttgcgtaca tcgtcatatt cctcagccgc
     4201 cagacgacac gcaggcaaca gctgtcccgg tacgggcaga gtggtcaggg tgtgaatggc
     4261 ggttcagtta aaatctggac tggagtttgg gtaaaacagg ctggttgctg ttaccgcact
     4321 gtggttgtca cacaatggcc cacctgcgta aaaaactgtc agcaggcagg gatggacatc
     4381 gggggatgtg ctgtatcggt tgccggaagg aatggtaaag aatgtgccat ggttaaatct
     4441 ccgtatggtt catacggaat agccacatcg taaaaatgcg cagtgtcgct gaaatggcct
     4501 ccggaaccga ctgtaacaat tagcgccagc ccgcagttgt cgttcagggg cttacaccag
     4561 agaacatatc tccgacaggg tactgtccag tcgcggtcgg ccccggttac ttttttcggg
     4621 aagcctgcta cgcctcagaa taataccgtg ttctttctga tcctgtttag ccaggtagct
     4681 gagggcgtaa cgtaagtcgt ctaccgcaga tttatcacta tagtgaatca cgtgatcgat
     4741 acgcaccggg tatttgtctt tagcccggca cagatgaaaa taaccctccc cttccgtgat
     4801 ccgcctccag atatccccca gttgcctgga tatctgataa gagttcttgt gacgttgccc
     4861 atccagatag ccgataaaat gaatatgaag ccctttgtct ggtgtatatt ccattaccca
     4921 gtaatatccc gccagcatcg tttgggtttc gctaagtaaa cggtatattt ccatacacat
     4981 actgtgctta caggaatgac caaaactggg cgtgtctttc ctgtaggcaa aatcaattct
     5041 gaaaggtaac agtttagaaa aacgttgaaa cagaccttcc atgtgttcat tcacatcttt
     5101 cagaatcgcg aaattcattt cgtaattggg gttagcatta tacattttat agtccttact
     5161 ttataaaact cacaggaaga ggaaattatg taaatgctga gggtacagaa acgtcgtacc
     5221 acggcattaa taaaagagat aaccagcagt acagacaacc tgttaaaact gttttgtatt
     5281 acctcattac agcaatgagg taatacttca gaatacagtg agactcactt aaactgagga
     5341 ccagtacgaa ttacaatggt aaaaacacac ctcatagaat taagtttaag gaagtaccat
     5401 tcaatcactt acaggaagta atctgacatt cacacaagtc agtatcgggt agctacaggc
     5461 aacaccgcta ttgatacccc acacaccata agaagattat ttcatacaga aaagattata
     5521 tttacccata cttataaact gtaacgctac tataacgtac gaaccaaaga taatatatcc
     5581 ctaaagtgtt ctggtacgta actagtgtta aatacgaacc agacacacac tcaatggatt
     5641 aactttctca atattacgct atgagtgagt gacataattt acacataact aatccccata
     5701 caccaccaca ctgtagcata tggggataga cgcattacgc acatacagaa tactcagcat
     5761 aaagaggagt gacattacga tagaaagaga caacataaca gataacataa ctcccattaa
     5821 taatactaat taacctaaat tatttattga atacgaaaac aatgaagtgc cctccttatc
     5881 taaggccaaa gccagcaata tcctgtacaa cactctatcc aatagtaaat agtagttaca
     5941 ccagttatat ttactgactg acctgtacca aagttctgta atacaacact gacatggtca
     6001 tggcattaat ctcctccgca agtaacggag gagactatat gttactcaac gattaagtcg
     6061 ttcagaggtc aaagttaggt gcgacacaca acgtttttcc ctcaataacc ggtacaactc
     6121 tgttttgttc atacagtaaa tccagtaacc agttgatttt atcctttttc cggaaacggt
     6181 tcggaccacg ctgtaaaatg tcattttttt tcatacagag gatccccttc tcaatgcaat
     6241 agctttttat ccagttgaaa agttcaagct cctctggaat gagtcgcaca ggtacggtca
     6301 gagcagtgtt gtcaaaagtt aacggattag acaaccgcat atactcatta ccgtaccata
     6361 ttgctaattc tctcgccatt tctgcagtgt aaggggaaat ctccccctct tcaccgctcg
     6421 aatggtaaat aagtcctgcc agtcttgcca tatactctgc atttttggca gcatattccc
     6481 gacaatgtct taaaggcccc aatcccccca gtttcgattc cacatcattg taataatccg
     6541 tccagattct ggcagcctga ggagaaaagt gaaggcaacg tcgttcacca ctcatcgcca
     6601 gactttcatc aataagttca ttgatcctct tatcaaataa ctcctgatac tgtgatgaat
     6661 aattatctcc ggttattatc cttgtcccct gcattgatgt tggttgacac atcaaaaacc
     6721 ttgcatgatg tcctgacgtt ttcacaagtt ctttttttcg cgtacaaaaa cctttgtggt
     6781 aaacatcagg ctgaatcatc gccgacatcg tcagtcttgg ctccttcaaa ttaattccgg
     6841 gagatgattt cctgtcgatg aaaagagaac ctccatccca caaagtgtta ataattccca
     6901 gtttactcat ggcccggctg tcaaaaatta ctcccccttc actggataca agagcaaaag
     6961 aacgattgct atcggagtaa tattttaaca ttccctctat cgttgtatca ttaaaaattg
     7021 ttcgacgtat ctgcggcgga acaggagagt tattcagatg cgtttcaagc tctgattctg
     7081 ttgccttgta atctttaccg gcacgaatct ctttatgaaa ttttgattcc agcgcttttt
     7141 gtttttgctc ccatatttcc ttttctgtac tgtaattctc aaccagtttc gcgtattctt
     7201 ccgccagggc ttcatccctg agataaaatg ctttcataaa cactttatcc acggtcgttt
     7261 tcctttcacc ggaatcagcc agaatcagag agtaaatatt aacgggccca cgtaaatttc
     7321 caggtctgca cacgtcaatc tgattctgac aggcaattga catcgctgtt aatgcggatg
     7381 tcgccaccat agccaaagga gcctgtgtat tttgttgagt ttcaattatt gcatttctca
     7441 ccagcggtgg tagtgcatat atcggataag gattttctgg tgctagtaag cacataaagc
     7501 ctccattttc attaatgggt tagtaaagtt gcagcaattt ccgctacaat agcgaaaata
     7561 gctgctattc agactgaaaa tacaaacagt tcatctggtc tgaatccacc agagaaggtc
     7621 acttagtaac cagccacaag atttctttcc gatagtctta cgcggtgggt agtttccctc
     7681 tcgttcaagt ttccaggctt gtgaacggga aattgaggtg atctcttttc gttccttttc
     7741 acgtaccagt ctgtcaccct cagtcccata ggctttcaaa attactgaac gttgctcatc
     7801 tgttaatgga acccacatga cggccctcct gatatatcaa tgattcttat ctcagttgtg
     7861 agcatgaaat gactgaattt cctggacaat ccatagtgga gattccgttg tcatattgct
     7921 cacagaacac ccatggacag caaggtacat acgaaaacaa atcaagtcaa gttaagacaa
     7981 ataaaacaat acttgactca caagcccact cataggtcaa tatgtgttga ttagcaacaa
     8041 aacaaccaat aatgcacatt acaaacaatg attttaagcc aagaagtggt tttcactgtt
     8101 gacaatgaca caaaatgaat ctagtatcga cttcatgtta tgctcagata aacttctgac
     8161 agtgatagta aagaagagaa ggagggggtt aaatttgtga ccaagatcac aaaaataaat
     8221 tagtttttac tattcacaat aggagggaaa gagagccagg cattttacct gtcattgaaa
     8281 tgacaacaac agcttcaaca gatcggaata ttcaggagga ggaagaagaa ggagatctga
     8341 cgggtgttat aattgttaca acacgctgga ggaaaattat cgtaataata gatgcattct
     8401 caggtatacc ctgtatcacc ccgaaaacac ggcaggcatc cgttttatta gtagatgcag
     8461 actcagtatc aacgtatcct ttcaggagat aacatcgatt ttaactgcca gtatccggta
     8521 tgcggcaaaa ggaaaccttc ttacataatg gtaaatcaga ctatgcaaca aaatcagtac
     8581 tatcgtattt tatccggaga atcccgccca ttccctggca tatatgaggc tgtttccgaa
     8641 caactccttt tgctcatttt taaggcacgt cagctatatc aggatccaga atatcgcccc
     8701 tggtttgaag aacagcacaa agttctcttg gcattgcgtg ataatacaga gctccagaaa
     8761 atttttaaca aacaaagaaa agaaaatttt gctcttggcg catctacatc gtctcaaccc
     8821 actgctctta ctaatgcgct taatgttatg tgccatttat acaatggctt tgatgaagga
     8881 aatatcagcc tgaaaagctg gggagcgatg caagaggtac agacaacacc agactttttc
     8941 agcctgggac caagaacctg gaaaacacct gaactggcaa agtttgtagg agatgattta
     9001 tttaatattc tgactgaatg taaagcctgg agttcaccaa ttccagcaga agcccggcag
     9061 gggtattgcc atcaggatct gtctgttgcg cactcctgca cctcagagtt gctggcacac
     9121 ctgaagcagt atcatgaaat agatgaatca acaatacttg ccataaccat tggtctggcg
     9181 gtcaacgaat tctaccagta taagcaaaat attccagaat ggctttcctg ttttgagaat
     9241 aagaacaaat ggccctggga ctgcaggaag tcagatactg aagatgtgca cagactgaga
     9301 gaacgactat cgataatcgg atgtacagaa ttcgtaaaaa atatgttctc agtcgccagt
     9361 cagcatgaaa taaagacaat gtacaatatt tatgtccata tttatgcagc tgaaatctgc
     9421 ggttgggtaa aaacaagcat ggcatatttt aaaccaacag atttcatcca gtacacatgg
     9481 aaattcgcaa cagttactcc ggataaagat gaaaaaaccg agaatacgac cgcatgggaa
     9541 agggattatt gtcagataac catacaacga gatgaactcc ttagattgat tcgcactaat
     9601 actctgcagg atgcagcaat tcatctttca ggctgcctgc aatatgcata tctggagtct
     9661 gtaaagaatg atattgacta tcctggaata gcccctaatg ctgaagaaaa gaaaatgaaa
     9721 tacacagatt ctattgaatt ttctggaatt aggaataaca tcataaatgc agtaaaacgg
     9781 ctggaatcat ttgaaaaatg gtatgaaact aataaagttt tgttggtaag taataaaaca
     9841 atacgaacag agaagattga cgttgttgta cgacttgcgg gactgaaagc ctatgatttg
     9901 caggaagggg ttcctgatgg tctgaaatgg aaagttaaag atggcataaa tgaacgcatc
     9961 aaaaccgata ccagtttatg cctgcctcag gatatcagtg atacatcgct aaaccgttat
    10021 cgtcacgctg tgacgcgcat tgtcaacgac gaaatcgaca cattactgat ggaacagaag
    10081 aaaaaaaata aaagattccc ctacagcaaa gatcgacatg cgatccaacc tttatggagc
    10141 aaatgcgtga tcgagcagta gagttctgag gggataaccg agctgttatc ccccgtttat
    10201 tctgccaact ctcaggtctg tcagagttcc aagaatacga tgcggctggc aaattatgcg
    10261 gcttttccat taacagattc ggccacaaga acaacaccgt gtttatttcg atcaggatgt
    10321 tcactccaga tttctgattc aggaatactt tccccgtcat agcagatccg atcgccatca
    10381 aacggataat accgtgcgga atcagtatct aaaataaaca ctttcagatt cgttgaacga
    10441 cgcgctatat gctgccagag ggctttacct ccttcatatt gctcgttatc acttaatagt
    10501 ataacgcccg cttttgtggc taccgtttca tatacaaacg tcgcaaggcc aacatcacgt
    10561 acagccccat caacaacaat cccttcaatc tgagcgacgt cctgctcttc ccagaatttc
    10621 cggacaaaca ccccttcaag ctgtgagcga cgctgaagct ccagagtggc aacaacttta
    10681 tggcgaatat caccatcaat aacagaaggt gcagcttcga ttaccgcagc gaacggagtc
    10741 tttggatccc agatgatctg gtaaagcacg ccctgaatag ttactccatc cagtatcgaa
    10801 aaacgacgag ctacagtagc aggggagtaa gacattggaa ttacagccat ttttgaatcc
    10861 cctctgagta atacaggcac gaaaatttac ctcaataatt atagtaaaca atacaagaac
    10921 taggtgcatc aacaataccc atcaattgta cgcaattcgt acagtaacca ggaactccct
    10981 caccttctgc caaacagatt gacaacatta ttgcctgttt gctgttgata gttattaacc
    11041 cgatcctccc agatctcaag tgctctgcgt ttttctggca aataatcgta tcgatcataa
    11101 tgttttgaac tgacatcgtt caacgcatga ttttgaatac gatcgcggat ttccttactg
    11161 attccagctt ctcccattaa cgttttacag gtccgacgta agtcacgtgc tgtaaaaatt
    11221 ttaaagtcag gattacaagc ccggaaatac atgatagcgc gagccagact gtccgtacga
    11281 accggacgct taccattagt cgaaagcggg aaaatgtaag ggctcgattt ttcttttgtt
    11341 aactcctgca cacaggataa ttcctgtaat gctgattcag tcaacggtat caaatgctct
    11401 cgtttatttt tagagacatc cgcagtcacc agcagcgttt tttgctgaaa atcaacatca
    11461 caccactgac tggcgatcat ttcaaaggga cgttgccctc cggtatagat acataatcga
    11521 atcaaatgtt gcatcaataa tccaacgttg gttgcttcag caaaatgttc cattagaaaa
    11581 cgcaactctt cgagtgtcag ccaggtgtca ccgacttttt ctgcagatga ctgtttcggt
    11641 atcactgata caggattggc ctccagccca aaactaatcc ctacacttgt gttcatcgga
    11701 tcgttatcag ctttcagacc atagttaaat gcagccatca ggtaagaacg gatccggtta
    11761 gagtgaacca cagcaccgcg ctgaatgatc ccggccaaga tatgtttaat ctgtccggaa
    11821 gtcacatctt tagctttagt ctcacggggg atcactgtat agcattcatt ttcaaggcgt
    11881 ttcagaacat cctgccatgt tctcttatta tcaattttca ttttgttaac ataaccatga
    11941 atcaatgctt caaatgaacc ctgtgattga tggagttgtc tccgctgctg ttctgcaagc
    12001 tgttgtcgct ccagttctgt ctggggatcc tttccttcaa tgagccaggc accatacttt
    12061 tttgccagtt cgttcgctgt ggccagtttc atctcaggcc atatacccaa ttgaatgaat
    12121 ctttcttttg tccctttttc cacgtaatag cggaaataga aaattttgct gccagaagac
    12181 tgaactttga cgccaagtcg tccagtgcca cgttgagagc tgttgctcca gacatagaac
    12241 gatgtctttt ttgcttttaa tgctcgaata gctgtttcag ttagattgac ggccatagaa
    12301 cctcttttct aaatcagtaa caatcaaaat aactcgtaaa tttgggtcag gtaatgggtc
    12361 aggtaacgat cgtacaatgg agaaaaaacg gaaccaagcg gaaacagaag aaattacata
    12421 attagttgat tataaacaat taataaaaca attacgaaca aaccaaaaca atacagaatg
    12481 gacttgcctc atgactcata atcgcttggt cgctggttca agtccagcag gggccaccag
    12541 atatagcaaa ggctgacgag aaatcgtcag cctttttctt tttatatatc agttactttg
    12601 cgtgccagta agccgctgca cgtacccgct gtgggtcata ctgttccgct tcaaagcggc
    12661 ggcttaaatt cttaacgact ttgccttcgc cggttatcca gatgaagtaa tcatcggcag
    12721 ggatttgcat ctgcgccaga cgcgcatcta ccgcctgctc atcatgcgcc agccattcga
    12781 tattaaaacc atcaaggtgc gcgagataat cctgacaggc gttatcccgc acgctaacca
    12841 gcgcactaac ttgcggttta acggcaagtt tgctcaacgt ttccaggcgg cggcgcagtg
    12901 cgggcattcc ggattcatcg cagacataca gctgatacgc gtaatcctct ggcaccacca
    12961 gcgaaccgcg cggacctgcc accgtgagtt tatcgcccgg ttgcgcctgc attgcccagc
    13021 tgctggcgac gccaccttcg tgaataaaga aatcaatcgc cagttcatgg cgtagttcgt
    13081 catacagcgg cgtatagtca cgcgacggtg ggcgtggtcc ttccggccag acgatgccct
    13141 cttccgttac cgttggcggc acaaagtgag catcagattg aggaaagaag agtttgctgt
    13201 gatcgtcaaa gccgcgcgat gtaaaaccgt ccagcgcctc gccgccaagg acaatgcgct
    13261 gaaaaccgac gctaatacgc tcaacgcgta gcacagtcaa ttcacggaag cgcagatcat
    13321 tgcgaacgcg ctgtgggtag cggggggtgt tattcattgt tatcgccttc atgatggtaa
    13381 tcagatatat ctaaataaaa tttcgcaaat gataatgatt gttaatcatg ataaatgcaa
    13441 gcgatttgta gaactgatat gtctatagtc tgataagacg aaccgcctct tctcaggcat
    13501 cattactcaa cgccggatgc ggcgtgaacg ccttatccgg cctacgtgtg agatgagtcc
    13561 acgtttaaaa atcattattg tacttgcagt aatgctcata tcagatataa attagatata
    13621 tctaattaaa caaaaggagg ctgatatgag ccatcatcac gaagggtgct gtaaacatga
    13681 aggccagcca cgccatgagg gctgctgcaa aggtgagaag tcagaacacg agcactgcgg
    13741 acacggtcac cagcatgaac acggtcaatg ctgcggtggt cgccacggtc gcggcggcgg
    13801 tcgtcgacaa cgtttctttg gtcacggtga attgcgtctg gtgattctgg atattctctc
    13861 gcgcgatgac agccacggtt acgagttgat taaagcgatt gaaaatctga cccaggggaa
    13921 ttacacccca agcccgggcg tcatctaccc aacgctggat tttctgcagg agcagtcgct
    13981 gattaccatt cgcgaagagg aaggtggtaa gaagcagatt gcgctgaccg aacaaggcgc
    14041 gcagtggctg gaagaaaacc gcgaacaggt agagatgatt gaagaacgca tcaaagcgcg
    14101 ttgcgttggc gctgcgctgc gccagaaccc gcaaatgaag cgggcgctgg ataattttaa
    14161 agcggtactg gatttacgcg tcaaccagag cgatatcact gatgcacaaa taaaaaagat
    14221 cattgcggtg atcgaccgcg ccgcttttga tattacgcaa ctggattaat caccgattgc
    14281 cggatgtggc gtgccctacc cccgtaggcc tgataagatg cgccagcatc gcatcaggca
    14341 ttgtgcgcca accgccggat gcggcatatc gattaatgca gcaccgtcac cgcgtcttcc
    14401 agtcggctgg cgcggtgttt caccatcgcc gacacctgcg cactctcttc cactaactcg
    14461 gcgtttttct gggtgatcag gttaagctca tccactgcac gggtcaggct ggaaagccca
    14521 tcggcctgtt ccagcgttga atggctaatc tgagctatca actgggtgac gttttttacc
    14581 tgtgccacaa tatcttccat cgtccgtccg gcggcgtgta cctgctgcga accggattgc
    14641 accttatcag cactggcatc aatcagcttg cgaatatcgt tggcagcatt ggcgctgcgg
    14701 ctagctaaat gacgcacttc ccccgccacc actgcaaaac ctttgccctg ttcgccggca
    14761 cgcgccgctt ccaccgccgc attcagggcc agaatattgg tctgaaacgc aatatcgtta
    14821 atcagcgaag taatggtgcc aatgcgctgg gtactgtcgg caatatcgtc cattgtcttg
    14881 atcaccgtag tcatcgcctc cccatcctgc actgccgcat tactggcagt gatagaaagt
    14941 ttatcggcag ccgacgccgt ggcagagttc tgtttcaccg acgccgccat ttggttcatg
    15001 gtcgccaccg tttgctgaac gttatcaact gtctgctggg tatgttcgtt cagttcatcg
    15061 gtgcctttcg ccagcgtctc actgccgttt ctgacactgg acacctggct tgagacatcg
    15121 ttaattagcc aacggcacat caggccaagc tgccctaccg cgcgtaatgt cagccccagc
    15181 tcatcgctgc gattcagatg ctcaacacta ttacgctctc cggtcgccac cttcagtgcc
    15241 tgacgggcga cattttctat cgggcggaca atctgccatt caaaacaagc gcttgccaat
    15301 aataccacta acgcacagag gatatacgtc accaccgggg cagcaacaaa ccatagcatg
    15361 gccgtcagca agataaacat cagggtcatc acgccacgcg cccgccagcg aagcggtaat
    15421 gaaggcagtt tacccagcca tcctttacgc accaccaggc ctttatgaat acgcttactg
    15481 gtacgtccgg cgttcaacgc tttgtacagc ggctccaccg ccgcgatctc ttcatccgtc
    15541 gcccgggtac gaatcgacat atagccactg atttttccct cgcgcaccat cggtaccgca
    15601 ttggcccgca cccaataatg gtcgccattt ttgcggcgat ttttcacgat gccgctccag
    15661 ggctcccctt ttttcagggt gaaccacata tccgcaaacg ccgcttttgg catatccggg
    15721 tgacgcacca tgttgtgcgg ttgaccttgt aactcttgca aggtatagcc gctcacctgc
    15781 acaaaagtgt cattagcatg agtgatatag ctttgcagat cggtagtgga catcagagtg
    15841 gtatcgtccg ccagcggggt attttgctgg gtgacatacg gatgagaaga catgttcgcg
    15901 tcctgtgcag gttatatggt tgttaacttc ttgtcagagt ttatgtcggc cccgctgcgg
    15961 ttatctttaa ctgattaatt tgatttagat cgcaatttgc gattaaagca taaatctaat
    16021 cccttgattt aaaatacttt cactctgtta ctatacgaaa acgttaatta tcttgcccaa
    16081 aaatcaggca gttgttgccc tgaaagtgtg catttgcgca gcaatcgcca aatccatacc
    16141 cgacaaaaac cgtgcaaaat aacaacagat gttaacaaat agcattaaat attgcgcaaa
    16201 tgataaccac atttgtgttt atcccgattt tcgcgatcgc agccggagtg gcgcaatccc
    16261 tgcaatactt aaatcggtat catgtgatac gcgagcctcc ggagcatatt ttgaacaggt
    16321 taccttcgag cgcatcggct ttagcgtgca gcgcccacgc cctgaatctc attgagaagc
    16381 gaacgctgga tcatgaggag atgaaagcac ttaaccggga ggtgattgaa tacttcaaag
    16441 agcatgtcaa tccggggttt ttagagtatc gcaaatctgt taccgctggc ggggattacg
    16501 gagccgtaga gtggcaagcg gggggtttaa atacgcttgt cgacacccag ggacaggagt
    16561 ttatcgactg cctgggaggt tttggaattt tcaacgtggg gcaccgtaat ccagttgtgg
    16621 tttccgccgt acagaatcaa cttgctaaac aaccgcttca cagccaggag ctgctcgatc
    16681 cgttacgggc gatgctggcg aaaacccttg cggcgctaac gcccggtaaa ctgaaataca
    16741 gcttcttctg taatagcggc accgaatccg ttgaagcggc gctgaagctg gcgaaggctt
    16801 atcagtcacc gcgcggcaag tttactttta ttgccaccag cggcgcgttc cacggtaaat
    16861 cacttggcgc gctgtcggcc acggcgaaat cgaccttccg caaacctttt atgccactgc
    16921 tgccagggtt tcgtcatgta ccgtttggca atatcgaagc catgcgcacg gctcttaacg
    16981 agtgcaaaaa aaccggtgat gatgttgctg cggtgatcct cgaaccgatt cagggcgaag
    17041 gtggcgtcat tctgccgccg ccgggatatc tcaccgccgt gcgtaagctg tgcgatgagt
    17101 tcggcgcact gatgatcctc gatgaagtgc aaaccggtat ggggcgcacg ggcaagatgt
    17161 tcgcctgcga gcatgagaac gtacagccgg acatcctgtg tctggcaaaa gccctcggcg
    17221 gcggcgtgat gccgattggc gcaaccatcg ccactgaaga ggtgttttcc gttctgttcg
    17281 acaacccgtt cctgcatacc accacctttg gcggcaaccc gctggcctgt gcggcggcgc
    17341 tggcgaccat caatgtgttg ctggagcaga acttaccggc tcaggctgaa caaaaaggcg
    17401 atatgttgct ggacggtttt cgtcaactgg cgcgggaata tcccgatctg gtacaggaag
    17461 cgcgtggtaa agggatgttg atggcgattg agtttgttga taacgaaatc ggctataact
    17521 ttgccagcga gatgttccgc cagcgcgtcc tggtggccgg aacactcaac aacgctaaaa
    17581 cgatccgcat tgaaccgcca ctgacactga ccattgaaca gtgtgaacag gtgatcaaag
    17641 cggcgcgtaa ggcgctggcg gccatgcgag tcagtgtcga agaagcgtag tatcaaatcg
    17701 gatggcgata cgacgtcgcc atccgatctt tttatacgat acgtacgccc gcaggcatca
    17761 tccgctccgg cgttaacagc acgctttcac tgccgtcatc ggtttccgca cacaacagca
    17821 tgcattccga cgtttcaccg cgcattttcg ctttttgcaa attgcataag accaccactg
    17881 tttttcccat cagctcttct tcgctgtagt acggcaccag gctggtcacg gtttgcagcg
    17941 ttttttcccc cacatcaacc tgtacgatgt acagcttgtc ggcgttttca tggcgtttca
    18001 cttccacaat ctttccgacg cgcatttcca gacgtgcaaa atcagcgtaa gccacggttt
    18061 ccattgtttc ctcccttcga gtaaaatttt actaaactat agcaaaagtt tttctcaatc
    18121 ctgtaggcta aaaatggaga atgcaggcgt gatcacattc gcaagccgct gtgttaccgt
    18181 tacagcgtca aagaaacgcg ctttatttac tgaaaacagg tgacccgata agcacttcct
    18241 ctacaatggg ggcgcacatc agggaaagta aaaaaggtaa acatggcaac actaaaagac
    18301 atcgcaatcg aagctggcgt atccctggcg acagtatcca gggtcttgaa tgacgatccg
    18361 acattgaatg tgaaagaaga gacgaaacat cgcattctcg agatcgccga aaagctggag
    18421 tacaagacca gtagcgcccg taaactccag acaggcgcag tcaaccaaca ccatattctg
    18481 gctatctaca gctaccaaca ggagctggag atcaacgatc cttactatct ggcgatccgc
    18541 cacggcattg aaactcagtg cgaaaagctg gcgatcgaac tgaccaactg ttatgaacac
    18601 aacggcttac cagacattaa aaacgtcacc ggtattttaa ttgtcggcaa acccacgtcc
    18661 gccctgcgcg ccgccgcctg tgcgttgacc gacaatatct gttttatcga ctttcacgaa
    18721 cccggcagcg gttacgatgc ggtggatatc gatctggcac gtatcagtaa agaaatcatc
    18781 gatttctata tcaatcaggg cgttaatcgt attggtttta ttggcggtga agacttgcct
    18841 ggcaaggcgg atattcgtga ggtcgccttt gcggaatatg gtcgtctgaa acacgtggta
    18901 cgcgaagagg atatctggcg cggcggtttt tccagttcgt cgggctatga actggcaaaa
    18961 cagatgctgg cgcgggaaga ctatccgaag gcactgtttg ttgcttccga ttccattgct
    19021 atcggcgtac tgcgggcaat tcatgaacgt ggcctgaaca tcccacagga tatttcgctt
    19081 atcagcgtta acgatatccc caccgcgcga tttacctttc cgccgctctc caccgtgcgc
    19141 atccattccg agatgatggg aagtcagggc gttaacctgg tgtatgaaaa agcccgcgat
    19201 ggtcgcgcgc tgccgctgtt ggtcttcgtt cccagcaaat taaaactgcg cggcacgacc
    19261 cgttaaatcc ccttgcacgc tgtccggcaa tcgtttttgc cggacagtgc tgccgtttat
    19321 tttcgtgatc cagttaaagt aaatgcattt atccgctact ttttagtaaa atttttacta
    19381 aactccccag caattacaca aactaccatc accataaatg gttccgattt ctctctaccg
    19441 ggaggcccta tgaatcgctg ggaaaacatt cagctcaccc acgaaaaccg acttgcgccg
    19501 cgtgcgtact ttttttcata tgattctgtt gcgcaggctc gtacttttgc ccgtgaaacc
    19561 agcagcctgt ttctgccctt aagcggtcag tggaatttcc acttttttga ccatccgctg
    19621 caagtgccag aagccttcac ctctgagtta atggctgact gggggcatat taccgtcccc
    19681 gccatgtggc aaatggaagg tcacggcaaa ctgcaatata ccgacgaagg ttttccgttc
    19741 cccatcgatg tgccgtttgt ccccagcgat aacccaaccg gtgcctatca acgcattttc
    19801 accctcagcg acggctggca gggtaaacag acgctgatca aatttgacgg tgtcgaaacc
    19861 tattttgaag tctacgttaa cggtcagtat gtggggttca gcaaaggcag tcgcctgacc
    19921 gcagagtttg acatcagcgc gatggttaaa accggcgaca acctgttgtg tgtgcgcgtg
    19981 atgcagtggg cggactctac ctacgtggaa gaccaggata tgtggtggtc ggcggggatt
    20041 ttccgcgatg tttatctgat cggaaaacaa ctcacgcata ttaacgattt caccgttcgt
    20101 accgactttg acgaagccta ttgcgatgcc acactttcct gcgaagtggt gctggaaaat
    20161 ctcgccgcct cccctgtcgt cacgacgctg gaatataccc tgtttgatgg cgaacacgtg
    20221 gtgcacagca gcaccattga tcatttggca attgaaaaac tgaccagcgc cagttttgct
    20281 tttactgtcg aacagccgca gcaatggtca gcagaatccc cttatcttta ccatctggtc
    20341 atgacgctga aagacgccga cggcaacgtt ctggaagtgg tgccacaacg agttggcttc
    20401 cgtgatatca aagtgcgcga cggtctgttc tggatcaata accgttatgt gatgctgcac
    20461 ggcgtcaacc gccacgacaa cgatcatcgc aaaggccgcg ccgttgggat ggatcgcgtc
    20521 gagaaagatc tccagttgat gaagcagcac aacatcaact ccgtgcgtac cgctcactac
    20581 ccgaacgatc cacgttttta cgaactgtgt gatatctacg gcctgtttgt gatggcggaa
    20641 accgacgtcg aatcgcacgg ctttgctaat gtcggcgata tcagccgtat taccgacgat
    20701 ccgcagtggg aaaaagttta cgtcgagcgc attgttcgcc atattcacgc gcagaaaaac
    20761 catccgtcga tcatcatctg gtcgctgggc aatgaatccg gctatggctg taacatccgc
    20821 gcgatgtacc atgcggcgaa ggcgctggat gacacgcgac tggtgcatta cgaagaagat
    20881 cgcgatgctg aagtggtcga tattatttcc accatgtaca cccgcgtgcc gctgatgaat
    20941 gagtttggtg aatacccgca tccgaagccg cgcatcatct gtgaatatgc tcatgcgatg
    21001 gggaacggac cgggcgggct gacggagtac cagaacgtct tctataagca tgattgcatt
    21061 cagggacatt atgtctggga atggtgcgac cacgggatcc aggcgcagga tgacaacggc
    21121 aatgtctggt ataaattcgg cggcgactac ggcgactatc ccaacaacta taacttctgt
    21181 cttgatggtt tgatctattc cgatcagacg ccgggaccgg gcctgaaaga gtacaaacag
    21241 gttatcgcgc cagtaaaaat ccacgcgctg gatctgactc gcggcgagtt gaaagtcgaa
    21301 aataatctgt ggtttaccac gcttgatgac tacaccctgc acgcagaggt gcgcgccgaa
    21361 ggtgaaacgc tcgcgacgca acagattaaa ctgcgcgacg ttgcgccgaa cagcgaagcc
    21421 cccttacaga tcacgctgcc acagctggac gcccgcgaag cgtttctcaa cattacggtg
    21481 accaaagatt cccgcacccg ctacagcgaa gccgggcatt ctatcgccac ttatcagttc
    21541 ccgctgaagg aaaacaccgc gcagcctgta cctttcgcac cgaataacgc ccgtccgctg
    21601 acgctggaag acgatcgttt gagctgtact gttcgcggct acaactttgc gatcaccttc
    21661 tcaaaaacga gtggtaaacc gacatcctgg caggtgaatg gcgagtcgct gctaacccgc
    21721 gagccaaaaa tcaacttctt caagccaatg atcgacaacc acaagcagga gtacgaaggg
    21781 ctgtggcagc cgaatcattt gcagatcatg caggaacatc tgcgcgactt tgccgtagaa
    21841 cagagcgatg gtgaagtgtt gatcatcagc cgcacggtta ttgccccacc ggtgtttgac
    21901 ttcgggatgc gctgcaccta catctggcgc atcgctgccg atggccaggt taacgtggcg
    21961 ctttccggcg agcgttacgg cgactatccg cacatcattc cgtgcatcgg tttcaccatg
    22021 gggattaacg gcgaatacga ccaggtggcg tattacggtc gtggaccggg cgaaaactac
    22081 gccgacagcc agcaggctaa catcatcgat atctggcgca gcaccgtcga tgccatgttc
    22141 gagaactatc ccttcccaca gaacaacggc aaccgtcagc atgtccgctg gacggcactg
    22201 actaaccgcc acggcaatgg tctgctggtt gttccgcagc gccccattaa cttcagcgcc
    22261 tggcgctata cccaggaaaa catccacgct gcccagcact gtaacgagtt gcagcgcagt
    22321 gatgacatca ccctgaatct cgaccaccag ctgcttggcc tcggctccaa ctcctggggc
    22381 agcgaggtgc tggactcctg gcgcgtctgg ttccgtgact tcagctacgg ctttacgttg
    22441 ctgccggttt ctggcggaga agctaccgcg caaagcctgg cgtcgtatga gttcggcgca
    22501 gggttctttt ccacgaattt gcacagcgag aataagcaat gaggatcatc gataacttag
    22561 aacagttccg ccagatttac gcctctggca agaagtggca acgctgcgtt gaagcgattg
    22621 aaaatatcga caacattcag cctggcgtcg cccactccat cggtgactca ttgacttacc
    22681 gcgtggagac ggactccgcg accgatgcgc tatttaccgg gcatcgacgc tattttgaag
    22741 tgcattacta cctgcaaggg cagcaaaaaa ttgaatatgc gccgaaagag acattacagg
    22801 tcgtggaata ttatcgtgat gaaactgacc gtgaatattt aaaaggctgc ggagaaaccg
    22861 tagaggtcca cgaagggcaa atcgttattt acgatatcca tgaagcatat cggtttatct
    22921 gcaataacgc ggtcaaaaaa gtggttctca aagtcaccat cgaagatggt tatttccata
    22981 acaaataaca actacggcgg caaaaggagt ttgccgccac cgctacccta ctcattttcg
    23041 gagatgtgtt atgtctgata ccaaacgtaa tacaatcggc aaattcggct tgctctcgct
    23101 gacttttgcc gccgtttaca gctttaacaa cgttatcaat aataatattg agcttggact
    23161 ggcctcggca ccgatgtttt tcctcgcgac gattttttat tttattccct tctgtctgat
    23221 catcgcagaa tttgtttcgt taaataaaaa ctcagaagcc ggtgtctacg cgtgggtaaa
    23281 aagttcgctg ggcgggcgtt gggcatttat tactgcctat acctactggt tcgtaaacct
    23341 gttctttttc acctcactgt tgccgcgcgt tattgcttat gcttcgtatg ccttcctcgg
    23401 ctacgaatat attatgacgc cggttgccac caccattatc agtatggtgc tgttcgcctt
    23461 ctccacctgg gtttccacca acggggcgaa aatgctgggg ccaattacct ccgtcacttc
    23521 aacgctgatg ctgctgttaa cgctctccta cattttactg gcaggtacgg cactggttgg
    23581 cggcgtacag cctgccgatc cgatcaccgt tgacgcgatg atcccgaact tcaactgggc
    23641 gttcctcggc gttaccacct ggatctttat ggccgcaggt ggcgcggagt ccgtcgcggt
    23701 gtacgttaac gacgtcaaag gcggttcgaa atcgttcgtt aaagtgatca tcctcgccgg
    23761 gatttttatc ggcgtactgt attccgtttc ctcggtgctg attaacgtct tcgtcagcag
    23821 caaagagttg aaatttactg gcggatcggt gcaggtattc cacggcatgg cggcgtattt
    23881 tggtctgccg gaagcgttga tgaatcgctt tgtcggtctg gtgtccttta ccgcgatgtt
    23941 cggttccctg ctgatgtgga ccgcaacgcc ggtgaaaatt ttcttctccg aaatcccgga
    24001 aggcatcttt ggtaagaaaa ccgtcgaact gaacgaaaac ggcgttccgg cgcgcgcagc
    24061 gtggatccag ttcctgatcg tcatcccgct gatgattatc ccgatgctcg gttccaatac
    24121 cgtgcaagat ctgatgaata ccattattaa tatgaccgcc gcagcgtcca tgcttccgcc
    24181 gttattcatc atgctggctt acctgaattt acgcgccaaa ttagatcacc tgccacgcga
    24241 tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt tcaatgctga ttgcgatatt
    24301 tgccgtaggg tttgtcgctt cgacattccc gactggcgcg aatattctga ccatcatttt
    24361 ttataacgtc ggcggtattg ttatcttcct cggctttgcg tggtggaaat acagtaaata
    24421 tataaaggga ttaacggctg aagagcgcca tattgaagcg acgccagcca gcaatgttga
    24481 ttaagcagaa taataaaaaa tgagcaggca ttttccctct tcctggatgg ggagagggaa
    24541 aatagtttct gcctttatat ttttataatt acaacgataa aaggctgtac tttttcttta
    24601 gctcatggat taacacaatg aaattaatca ctgcaccatg cagagcatta cttgcgctgc
    24661 cgttttgcta cgccttttct gtgaccgctg atgaagcacg tccggcagaa catgacgaca
    24721 caaaaacccc cgcaatgacc tcgacatctt ctccttcatt tcgtttttac ggcgaattag
    24781 gggttggtgg gtatatggat ttagagggtg agaataaaca taaatacagc gacggcacct
    24841 atattgaagg tggcctggag atgaagtacg gatcctggtt cggcctgatt tacggcgaag
    24901 gctggaccgt gcaggccgac cacgacggta atgcctgggt gcctgaccat agctggggcg
    24961 gtttcgaagg tgggattaac cgtttctatg gcggttatcg taccaatgat ggcaccgaaa
    25021 tcatgctcag tctgcgtcag gattcctcgc tggatgacct gcaatggtgg ggcgatttca
    25081 cgcccgatct gggctacgtc attcccaata cccgcgacat tatgaccgcg ctgaaggtgc
    25141 agaacttaag cggcaacttt cgttatagcg ttaccgccac acctgccgga catcatgacg
    25201 aaagcaaagc ctggctacat tttggcaagt acgatcgcta cgacgacaaa tacacctatc
    25261 cggcaatgat gaacggttac atccagtatg accttgccga aggcatcacc tggatgaacg
    25321 gtctggaaat caccgacggc acaggccaac tctatctgac gggcctgctt acccctaact
    25381 ttgccgctcg cgcctggcac cataccggac gcgccgacgg gctggacgta tcgggaagtg
    25441 aaagcgggat gatggtgagc gccatgtatg aagcgttaaa aggcatttat ctctccaccg
    25501 cttacaccta cgccaaacat cgccctgacc acgctgacga tgaaaccacc tctttcatgc
    25561 agtttggtat ctggtacgaa tacggcggcg gacgtttcgc aacggctttt gatagccgct
    25621 tctacatgaa aaatgcctct cacgatccca gcgaccaact cttcctgatg caatatttct
    25681 actggtaata aggactgtaa tttcatgaaa atcaaaacta ttttaacgcc agtaacctgc
    25741 gctctgctga taagtttttc cgcccatgcc gctaacgccg acaattataa aaacgtgatt
    25801 aaccgtactg gcgcgccgca gtacatgaag gattacgatt acgacgatca ccagcgtttt
    25861 aatccgtttt tcgatctcgg agcctggcat ggtcatctgt tgccagacgg cccgaacacc
    25921 atgggcggtt ttccaggcgt tgcgctgctg acggaagaat acatcaactt tatggccagc
    25981 aatttcgatc gtctgaccgt ctggcaagac ggcaagaaag tcgacttcac gctggaggca
    26041 tacagtattc ccggcgcgct ggtgcaaaaa ctgacagcaa aagatgtgca ggtcgaaatg
    26101 actctgcgct tcgccacgcc gcgcacgtca ctactggaaa ctaaaatcat cagcaataaa
    26161 ccgctggatc tggtgtggga cggcgagctg ctggaaaaac tggaagcgaa agaagggaaa
    26221 ccgctttccg ataaaaccat tgctggcgaa taccctgact atcagcgcaa aatcagcgcc
    26281 acccgtgatg gcctgaaagt cacctttggc aaagtgcgcg ccacctggga tctgctgacc
    26341 tccggtgaat cagaatatca ggtgcataaa tccctgccag tgcagactga aatcaacggt
    26401 aatcgcttta ccagtaaggc gcatatcaac ggttcggcca cgctctatac cacctactcc
    26461 cacctgctga ccgctcagga agttagcaaa gagcaaatgc agatccgcga tattctggcg
    26521 cgtccggcgt tttatctcac cgcctcgcag caacgctggg aagagtatct gaagaaaggg
    26581 ttaaccaatc cggatgcgac gccggaacag acgcgcgtcg cggtgaaagc gatcgaaacg
    26641 ctcaacggta actggcgctc gcctgggggg gcggtgaaat ataacaccgt cacgccgtcg
    26701 gtgaccgggc gctggttctc cggcaatcag acctggccgt gggatacctg gaagcaggca
    26761 tttgcgatgg cgcatttcaa tccggatatc gccaaagaga atatccgcgc ggtcttctcc
    26821 tggcagatcc agccaggcga ccgcgtgcgt ccgcaggatg tgggctttgt ccccgacctg
    26881 atagcatgga accttagccc cgagcgtggc ggcgatggcg gcaactggaa caaacgtaat
    26941 accaaaccca gccttgccgc ctggtcagta atggaagtgt ataacgtcac ccaggataaa
    27001 acctggctgg cagagatgta cccgaaactg gtggcctatc acgactggtg gctacgtaac
    27061 cgcgatcaca acggcaacgg cgtgccggaa tatggcgcga cccgcgacaa agcccacaac
    27121 actgagagcg gcgagatgct gtttacggtg aagaaaggca acaaagcaga gacgctgtct
    27181 gggctgaaca actacgcccg cgtggtagag aaaggccagt acgacagtct ggaaatcccg
    27241 gcacaggttg cggcgtcgtg ggaatcgggg cgtgatgatg ccgccgtctt tgggtttatc
    27301 gacaaagaac agctggataa atatgtcgct aacggcggca aacgtagcga ctggacggtg
    27361 aaattcgccg aaaaccgcag tcaggacgga acgttgctgg gctactcgct attgcaggag
    27421 tcggtggatc aggccagcta tatgtacagc gataaccatt atctggcgga gatggcgacg
    27481 attctcggta agccggaaga ggccaaacgc tatcgtcagt tagcacaaca gctcgcggac
    27541 tacatcaaca cctgtatgtt cgacccgact acccagttct actatgacgt gcgcattgaa
    27601 gataaaccac tggcgaacgg ctgcgcgggc aaaccgattg tcgagcgcgg caaagggccg
    27661 gaaggctggt cgccgctgtt taacggtgcg gcaacgcagg ccaacgccga cgcggtggtg
    27721 aaggtgatgc tcgatcctaa agagttcacc acctttgtac cgctgggaac ggcggcgtta
    27781 accaatccgg cctttggcgc agatatttac tggcgcgggc gcgtgtgggt ggatcagttc
    27841 tggtttggtc tgaaagggat ggagcgttac ggttatcgcg atgatgccct gaagctggcg
    27901 gatacgttct tccggcacgc caaaggatta accgccgatg gcccaattca ggagaattac
    27961 aacccgctga ctggcgcaca gcaaggcgca ccaaatttct cctggagtgc cgcgcatttg
    28021 tatatgttgt ataacgattt tttccgtaag cagtaatttt tcccgatgcc ggacgactgt
    28081 attaccgccg tccggcatga cgaacgggcg tattcttttt gaatcccatc acaaaccccg
    28141 cactcccctt ttcccttttc tccagcgacg gctaaattag aactcatccg accacataac
    28201 aattatttta catactggac aattttatga gctacccgtc gctgttcgcc ccgctggatt
    28261 taggcttcac cacgttaaaa aaccgagtac tgatgggttc aatgcacacc ggactggagg
    28321 aatacccgga cggcgctgaa cggctggcag cgttttacgc ggaacgcgcc cgtcatggcg
    28381 tggcgttgat tgtcagcggc ggcattgcac cagatttaac aggcgttggc atggaaggcg
    28441 gcgcaatgct caacgacgcc agccagatcc cacaccatcg caccattacc gaagccgtac
    28501 atcaggaagg cggcaaaatt gccttacaaa ttttgcatac cgggcgctac agctaccaac
    28561 cgcatctggt cgctccctcc gcattgcagg cccccatcaa ccgtttcgtg ccccatgagt
    28621 taagccatga agagatcctg caactgatcg acaatttcgc ccgctgcgcg caactggcgc
    28681 gggaggccgg atacgacggc gtagaagtga tgggttccga agggtatttg atcaacgagt
    28741 ttctgaccct gcgcaccaac cagcgtagcg accagtgggg cggcgattac cgcaaccgga
    28801 tgcgctttgc agttgaagtg gtgcgagcag tgcgcgaacg cgtcggcaat gacttcatta
    28861 ttatctaccg tctgtcgatg ctcgacctgg tagaaggcgg cggtactttt gcagaaacgg
    28921 tagggctggc gcaggccatt gaagcggcgg gcgcgaccat tatcaacacc ggcattggct
    28981 ggcatgaagc acgtattccg accattgcca cgcccgtgcc gcgcggtgcc tttagctggg
    29041 tcacgcgcaa actgaaaagc cacgtctcgc tgccgctggt gaccaccaac cggattaacg
    29101 atccgcaggt tgccgacgac attctctcgc gcggtgatgc cgatatggta tcgatggcgc
    29161 gaccgtttct tgctgatgcg gaactgctgt caaaagcgca atcggggcga gccgatgaga
    29221 tcaacacctg tattggctgc aatcaagcct gcctcgatca gatcttcgtt ggcaaagtca
    29281 cctcgtgcct ggtgaatcct cgcgcctgcc acgaaaccaa aatgccgatc cttcccgccg
    29341 tgcagaaaaa aaatctggcg gtggtcggcg ctggtccggc tgggctggcg tttgccatta
    29401 acgcggcggc acgtggtcat caggtaacgt tgtttgatgc tcacagtgag atcggcggtc
    29461 agtttaatat cgccaaacag atccctggca aagaggagtt ttacgaaacg ctgcgctatt
    29521 accgccggat gatcgaagta acaggcgtga cgctgaaact caatcacacc gtgacggcgg
    29581 atcagttaca gtcgttcgat gaaacgatcc tcgctagcgg gatcgtaccg cgcattccac
    29641 ctatcgacgg aatcgatcat ccgaaggtgt tgagttatct cgacgtactg cgcgacaaag
    29701 cgcctgtggg caaaaaggta gcaatcatcg gttgtggcgg gattggtttt gatacggcga
    29761 tgtttttaag tcagccgggt gagtccacca gccagaacat tgccgagttc tgtaatgagt
    29821 gggggatcga cagcagcctg caacaggctg gtggcttaag cccgcaggga atgcagatcc
    29881 cgcgtagccc acggcagatt gtgatgctcc agcgcaaagc cagcaaaccg ggacaggggt
    29941 taggcaaaac caccggctgg atccatcgca ccaccctgct ctcgcggggt gtgaaaatga
    30001 tcccaggcgt aagttatcag aagattgacg atgacgggct gcatgtggtg atcaacggcg
    30061 aaacgcaggt attagcagtg gacaatgtgg tgatctgcgc agggcaagag ccaaaccgcg
    30121 cgctggcgca acagctgatt gatagcggga aaactgtgca tttaattggc ggctgcgatg
    30181 tagctatgga gcttgacgca cgacgggcaa ttgcccaggg aacacggctg gcgctggaga
    30241 tttaaatcgt tgtttgaagc gccggatgcg acgcatccgg cacgtcgcgt taatcaataa
    30301 acaaggcggg agaaatgccg aatcgcgttg ccagtttttt agcgtgttcc agcgttaatt
    30361 tcctcttccc gctcaaaacg cgtgacacca tggatttact gccaatttcc ggaagatcgg
    30421 aaagggttaa accatattga tccataaggg tacgaatcac ggctataccg ccaggcatgg
    30481 cttgagccat ggcattaaat tcagcaaatt cgggcgctga ttcttcccac gcggttattt
    30541 tggcacacac cagatccagc aagggatttt caggatcgtt gagcagcaga tgatcgacca
    30601 gttccagcgc ctgggcgtat tgttcctcgt tctgaatacc cgccagaaaa ggtgccacag
    30661 cagttagctt ttctcctgct tgcaagatgt cggcaatcgc aatcattttt tccccttagt
    30721 acgatgaacg gcggtaaaga aatcgtattc tttatgcgtc ataacttcac gtatgtagca
    30781 taaacgtaga aaaacgctaa acaagagaat gtagaggatg gtgacaggga ttttttgttt
    30841 tatggaagcg cgattccaga actgggcaga cattgccgga tgcgacgctt gccgcgtctt
    30901 atccgacctg ggggtaatgt gctgaacgta ggccggataa ggcgtttacg ccgcatccgg
    30961 caggtaactc ccaatcttat cgacgacgcc ccagcttcac tgctttcagc accacaaatt
    31021 tattattcgt ggcgatggtg gtgcagttgc cgaaaatttt cttcagttta tggaagtaat
    31081 ccaggtgacg gttggcaacg atatacagct cgccgttgat tttcaggcag cggcgggcat
    31141 ggtggaacat ctcccaggcg acgttatcgg tcagcgcatg ttgctgatga aacggcgggt
    31201 tgcagagcac agcattaaaa cggaaaggct ccacgccgga gagcgcgttg ttgatcataa
    31261 actcgcagcg atccaacgcc tcgggcatgt tggtttcaac gttcaaacgg ctggaagcaa
    31321 ccgccatcgg tgattcatcg acaaacacca ctttcgcctg cgggttttta tcgagcaatg
    31381 tcaggccaat aacgccatta ccgcaaccga gatcgacaat ctcaccttcg agattctctg
    31441 gcagatgttg cataaagaag cgcgcgccaa tatccagccc agtgcgggag aaaacattcg
    31501 catggttgtg gatagtccag tcagtgcctt ccagcttcca gctaacggtc tgcggcgcat
    31561 cggccaacgg cggttcgttg aaggtgcagt taatcaggcg cgctttcttc catgccagcg
    31621 tggtggtggt cggaccgagc actttttcga acagttccag cgtggaagtg tggatgtcac
    31681 gggctttggc accggcaata atacgtgtat ccggcgtgac cactttgcgc agcgcacgca
    31741 gttgttgttc cagcaacgcc agtgttttcg gtactttgat cagtaccacg cccggctgtt
    31801 gcgggtagtc ggcggtgctg tcgagaaact tcacgctcga ctcatcaatc ccgttgaggc
    31861 gtaaattctc gcgcgtcgcc agttcgctga tgtatgagtc gccaatgctg tatggcttat
    31921 gttccgccag cgcacaactt aacgcaccaa aggcatcatt caggatcaac accgggccgc
    31981 ggatttctgt gtcgtccaac tgttgcagca aatattcatc cgccgcttcc cacgcctgta
    32041 gtgggttaac gtcatccgtc gccggaaaac gttgtagtgt cagtgaacgg aaaccgttgt
    32101 ctaagtggct catcggccct cctgaatgat aaaatttcga cgttgcccct gaaaagggtg
    32161 cgtgagtata ccctttttct ctttttcgtg ggcggttatg agcaatctta cttatcttca
    32221 gggttatccc gagcagctac tttcccaggt gcgaacgctg attaacgaac agcgtctggg
    32281 agatgtgctg gcaaaacgct atccgggaac acacgactac gccaccgata aagccctctg
    32341 gcaatatact caggatctta aaaatcagtt tctgcgcaat gccccgccga tcactaaagt
    32401 gatgtatgac aataagatcc acgtgctgaa aaacgcgctc ggattacata ctgccgtttc
    32461 gcgtgtgcag ggcggcaagc tgaaagcaaa ggcggagatc cgcgtcgcta ccgtgtttcg
    32521 caacgcgccg gaaccgtttt tgcgcatgat cgtggtgcac gagttggcgc acctgaagga
    32581 gaaagagcat aacaaagcgt tctatcaatt gtgctgccat atggagccgc agtatcacca
    32641 gcttgagttc gacacccggc tgtggctaac gcagttgtcg cttgggcagg acaaaatctg
    32701 aaaatgatgt caggcgttat cttttcttcc tatagcgcct aaaatcgacc tccccccttt
    32761 cgttaaggat aacgaccatg caggcccgcc ttcttctccg tatttgtttt tctcgccgga
    32821 ctcttaaaat tggctgttta ttgttgctta tagcaggagc gaccattttt attgccgatc
    32881 gcgttatggt caacaccagc aagcagctga cctggagcga tgtcagcgca gttccggcgc
    32941 gtaacgtggg tttattgctg ggggcgaggc cgggtaatcg ctactttacc cgacgtattg
    33001 ataccgcagc agcgttgtac cacgccggaa aagtgaaatg gctactggta agtggcgata
    33061 acgggcgtaa aaattacgat gaagcctcag gtatgcagca ggctttgatc gctaaaggcg
    33121 taccggcgaa agtgatcttc tgcgactatg ccggattctc aacgcttgat tcggtggtgc
    33181 gtgccaaaaa ggtgtttggg gaaaaccata tcaccattat ttcgcaagaa tttcataacc
    33241 aacgcgccat ctggctggca aaacagtacg gcatcgacgc tatcggtttt aacgcgccag
    33301 acctcaatat gaagcacggt ttttatactc aactgcggga gaaactcgct cgcgtcggcg
    33361 cggtgattga cgcaaaaatc ctccatcgcc agccgaaata tctcgggcca tcggtcatga
    33421 ttgggccgtt tagtgagcat ggctgtccgg cgcagaaata atgcgtatct gcgcacgtcg
    33481 aagatgaaaa aggcgtgcta cattgacggc agaatccctt tatggagtat ccacgcgtta
    33541 tgatacgttt cgctgtgatt ggtacgaact ggatcactcg ccagttcgtc gaggccgccc
    33601 atgagagcgg taaatacaag ttaaccgccg tatattcccg cagccttgaa caggcccagc
    33661 actttgccaa tgatttttct gtcgagcatc tgtttacgtc gctggaagcg atggcggaaa
    33721 gcgatgccat tgacgcggtg tatattgcca gcccgaatgc cctgcacttt tcccagacac
    33781 aacttttcct tagtcacaaa attcatgtga tttgcgagaa gccgctggca tcgaatctgg
    33841 cggaagtgga tgccgccatt gcctgtgcgc gggaaaatca ggtggtgctg tttgaggcat
    33901 ttaaaacggc ctacctgccg aactttcatc tgttgcgcca ggcgctgccg aaagtcggca
    33961 aactgcgtaa agtctttttc aactattgcc agtattcctc gcggtatcaa cgttacctgg
    34021 atggtgagaa tcccaacacc tttaatccag cattctctaa cggttcaatt atggatatcg
    34081 gcttttactg tctggcgtcg gcggtggcat tattcggtga accgaaaaac gtgcaggcaa
    34141 ccgccagttt gctggcaagc ggtgtggatg cccacggcgt ggtggtgatg gattacggtg
    34201 atttcagcgt tacgttgcag cactcaaaag tgagcgattc tgtcctggca agcgagattc
    34261 agggcgaagc gggatcgctg gtgattgaaa aactgtctga atgccagaaa gtgtgttttg
    34321 tgccgcgtgg cagccagatg caggatctga cccaaccgca gcatattaat accatgctct
    34381 acgaagcaga gctgtttgct gaactggtgg atgagcatct ggtggatcat ccagggctgg
    34441 cggtcagtcg catcaccgct aaactgctga ccgagatccg ccgccagact ggggtgattt
    34501 ttccggcaga tagcgtaaaa ctataattgc caaagtaaaa cagtgtaaaa ggtatgtaac
    34561 agaccattga ctgactgaat ggtctgtcat actttgttac ctgcaaaggg gagtaacttc
    34621 attgccggtc gatcgtcatt acgatgtgtg aaaaaccaca tccggtcacc gggcaacccg
    34681 aaaggaatac gcagacgtat tccttttttg ttgtaagtga gaccttgccg gaaggcgagg
    34741 tctatgcata aaaagcagcg gctgacgtct tccgacgttg gccgtttttt tatgtgtaag
    34801 gaacttctat gaatactgtc ggcacgccgt tgctatgggg cggattcgct gttgttgtca
    34861 ccattatgct ggctatcgac ctgttgttgc aggggcgtcg tggggcacat gccatgacca
    34921 tgaaacaggc tgcggcctgg tcgctggtct gggtgacgct gtcgttactg tttaacgccg
    34981 ctttctggtg gtatctggtg caaaccgaag gtcgcgccgt tgccgatcca caggcactgg
    35041 cctttctcac gggttatctg attgagaaat cgctggcggt cgataacgtc tttgtctggc
    35101 tgatgttgtt cagctatttc tctgttccgg cggcattaca acgccgcgtg ctggtgtatg
    35161 gcgtgctcgg ggcgattgtt ctgcgtacca tcatgatctt cactggcagc tggctgattt
    35221 cacagttcga ctggatcctg tatatcttcg gtgccttcct gctgtttacc ggcgtgaaga
    35281 tggcgctcgc ccatgaagat gaatcaggca ttggcgacaa gccgctggtg cgctggctac
    35341 gcggtcattt gcgcatgacc gacaccatcg acaacgagca tttctttgtg cgtaaaaatg
    35401 gtttgttgta tgccacgccg ctgatgctgg tgctgattct ggtggagttg agcgacgtga
    35461 ttttcgccgt ggatagtatt ccggctatct tcgccgtaac caccgatccg tttattgtgc
    35521 tgacctcaaa cctgtttgcg atcctcggcc tgcgtgcgat gtatttcctg ctggcaggtg
    35581 tggcagagcg tttctcgatg ctcaaatatg gtctggcggt gattctggtg tttatcggta
    35641 tcaagatgct gattgtcgac ttctaccata ttccgatcgc cgtctcgctg ggcgtggtgt
    35701 ttggcattct ggtgatgacg tttattatca acgcctgggt gaattatcgg catgataagc
    35761 agcggggaga atgattcgct gtatattaag ccattactag agataactca ttccagacaa
    35821 aggtgagcta ttaattatgt aatcggctta tagctcttta caaaaagacc gcacgggtgt
    35881 tggtggcgtc aaaatcgccg aagcgttccc tggaggccgg ggcgacgcgc atggatgcgc
    35941 ggcgagggcg catcttacag ggacgttccc ttgcgcccga cccggtagcc gcaagggata
    36001 agctgaggg
//
LOCUS       DAZGHF010000036        35562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGHF010000036 DAZGHF010000000
VERSION     DAZGHF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.036"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            206..796
                     /locus_tag="ACHK3A_001883"
     CDS             206..796
                     /locus_tag="ACHK3A_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8739964.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFSNSKGTLNNDMSINNPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDATS
                     KTATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
     gene            905..1645
                     /gene="yadV"
                     /locus_tag="ACHK3A_001884"
     CDS             905..1645
                     /gene="yadV"
                     /locus_tag="ACHK3A_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO8739965.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGIPSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDVKMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            1680..4280
                     /gene="htrE"
                     /locus_tag="ACHK3A_001885"
     CDS             1680..4280
                     /gene="htrE"
                     /locus_tag="ACHK3A_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO8739966.1"
                     /translation="MTIKSTKKYHNHLTRIATFCALLYSNSALCAELVEYDHTFLMGK
                     DASNIDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFTVIEGKKNAQACITQKNLL
                     QFHISSPDKNSEKAILLKRDEDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASG
                     NYNWMTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPVLASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVT
                     IEEADGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNN
                     YLTGYTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWN
                     KLFENTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLN
                     EDGQTDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTN
                     RISYSVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADI
                     YDEQNNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSI
                     ILNGLRCQIQ"
     gene            4297..4869
                     /locus_tag="ACHK3A_001886"
     CDS             4297..4869
                     /locus_tag="ACHK3A_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8739967.1"
                     /translation="MIKTTPHKIAILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPKMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALRETTPGQGIPGKFRAQATFNFIYQ"
     gene            4881..5489
                     /gene="yadL"
                     /locus_tag="ACHK3A_001887"
     CDS             4881..5489
                     /gene="yadL"
                     /locus_tag="ACHK3A_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO8739968.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDIEMG
                     TCTSTLTDDGTAKLSTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTPR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAG
                     ATGGDVVDYPMSAVLVVAKDKTVTDVTAGDFTAPATFTVTYN"
     gene            5516..6112
                     /locus_tag="ACHK3A_001888"
     CDS             5516..6112
                     /locus_tag="ACHK3A_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO8739969.1"
                     /translation="MRPTQRNLMKKSILLLSLLFSTTFPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNTGAKNVGVVIFSIQDPANTFNVLGVTGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGQLISHVLVDIYYE"
     gene            6138..7427
                     /locus_tag="ACHK3A_001889"
     CDS             6138..7427
                     /locus_tag="ACHK3A_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO8739970.1"
                     /translation="MQHDNGVKMKIFFRYFLFLALCSCCYTASAGTDDNVSYLVGNDY
                     GVGPGDQKWRETGPSADITVKFRYSTSTNNLVFYKPTQLGPTGVSLYWSQLNSASGGG
                     YLYCNRSNTASGEPMAIENQMVDSGKRYGGHKLFETSVPGLYYTLKISNIWSSYTTTN
                     ISSSGMYIGDSPTQYFRWIPESEATLYWSCNNANSSNKYWAVGGVWQSLTVEFYTDTN
                     FDPTKTNKVTLSKPGGYLYSFKGYGLGGGINDHSYFLKIDFDLDNMTLSNPTCFTAAL
                     TGPSVSGSTVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKEL
                     LANTLTGNNAAKGVGVLIEGLKNTKSAQMVLKPNDSTSVYKDYETEDDTTGGIYPDKG
                     NGTSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            7539..8333
                     /gene="panB"
                     /locus_tag="ACHK3A_001890"
     CDS             7539..8333
                     /gene="panB"
                     /locus_tag="ACHK3A_001890"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO8739971.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8345..9196
                     /gene="panC"
                     /locus_tag="ACHK3A_001891"
     CDS             8345..9196
                     /gene="panC"
                     /locus_tag="ACHK3A_001891"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO8739972.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(9278..9481)
                     /locus_tag="ACHK3A_001892"
     CDS             complement(9278..9481)
                     /locus_tag="ACHK3A_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8739973.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYKEGLQKVRLAQRLLSKGM
                     SREDVAEVTALSLAEIDKLINSN"
     gene            complement(9550..10506)
                     /locus_tag="ACHK3A_001893"
     CDS             complement(9550..10506)
                     /locus_tag="ACHK3A_001893"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO8739974.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGRQEERQEVRQEVRQEVRQEVRQEV
                     IQEVRQEVRQEYALRFLSKGMSREDVAEMANLPLAEVDKLIN"
     gene            10780..11160
                     /gene="panD"
                     /locus_tag="ACHK3A_001894"
     CDS             10780..11160
                     /gene="panD"
                     /locus_tag="ACHK3A_001894"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO8739975.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(11164..12393)
                     /gene="yadE"
                     /locus_tag="ACHK3A_001895"
     CDS             complement(11164..12393)
                     /gene="yadE"
                     /locus_tag="ACHK3A_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO8739976.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYTTLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12457..12897)
                     /gene="yadI"
                     /locus_tag="ACHK3A_001896"
     CDS             complement(12457..12897)
                     /gene="yadI"
                     /locus_tag="ACHK3A_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO8739977.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLV
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(13002..13772)
                     /gene="yadH"
                     /locus_tag="ACHK3A_001897"
     CDS             complement(13002..13772)
                     /gene="yadH"
                     /locus_tag="ACHK3A_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8739978.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13769..14695)
                     /gene="yadG"
                     /locus_tag="ACHK3A_001898"
     CDS             complement(13769..14695)
                     /gene="yadG"
                     /locus_tag="ACHK3A_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8739979.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14804..15466
                     /gene="can"
                     /locus_tag="ACHK3A_001899"
     CDS             14804..15466
                     /gene="can"
                     /locus_tag="ACHK3A_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO8739980.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15507..16043)
                     /gene="hpt"
                     /locus_tag="ACHK3A_001900"
     CDS             complement(15507..16043)
                     /gene="hpt"
                     /locus_tag="ACHK3A_001900"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO8739981.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            16249..18639
                     /gene="gcd"
                     /locus_tag="ACHK3A_001901"
     CDS             16249..18639
                     /gene="gcd"
                     /locus_tag="ACHK3A_001901"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO8739982.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILAWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKNLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18835..20385)
                     /gene="cueO"
                     /locus_tag="ACHK3A_001902"
     CDS             complement(18835..20385)
                     /gene="cueO"
                     /locus_tag="ACHK3A_001902"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO8739983.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     VIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20551..20898
                     /gene="yacC"
                     /locus_tag="ACHK3A_001903"
     CDS             20551..20898
                     /gene="yacC"
                     /locus_tag="ACHK3A_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO8739984.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            21004..21870
                     /gene="speE"
                     /locus_tag="ACHK3A_001904"
     CDS             21004..21870
                     /gene="speE"
                     /locus_tag="ACHK3A_001904"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO8739985.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21886..22680
                     /gene="speD"
                     /locus_tag="ACHK3A_001905"
     CDS             21886..22680
                     /gene="speD"
                     /locus_tag="ACHK3A_001905"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO8739986.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITTALWKEMREIYYGRNMPAV"
     gene            complement(22718..23080)
                     /gene="yacL"
                     /locus_tag="ACHK3A_001906"
     CDS             complement(22718..23080)
                     /gene="yacL"
                     /locus_tag="ACHK3A_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO8739987.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(23255..25852)
                     /gene="acnB"
                     /locus_tag="ACHK3A_001907"
     CDS             complement(23255..25852)
                     /gene="acnB"
                     /locus_tag="ACHK3A_001907"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO8739988.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            26207..27964
                     /gene="yacH"
                     /locus_tag="ACHK3A_001908"
     CDS             26207..27964
                     /gene="yacH"
                     /locus_tag="ACHK3A_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO8739989.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQEVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPTNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHSAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSDHTVRLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(28035..29459)
                     /gene="lpdA"
                     /locus_tag="ACHK3A_001909"
     CDS             complement(28035..29459)
                     /gene="lpdA"
                     /locus_tag="ACHK3A_001909"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO8739990.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29784..31676)
                     /gene="aceF"
                     /locus_tag="ACHK3A_001910"
     CDS             complement(29784..31676)
                     /gene="aceF"
                     /locus_tag="ACHK3A_001910"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO8739991.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKDVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31691..34354)
                     /gene="aceE"
                     /locus_tag="ACHK3A_001911"
     CDS             complement(31691..34354)
                     /gene="aceE"
                     /locus_tag="ACHK3A_001911"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO8739992.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34515..35279)
                     /gene="pdhR"
                     /locus_tag="ACHK3A_001912"
     CDS             complement(34515..35279)
                     /gene="pdhR"
                     /locus_tag="ACHK3A_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO8739993.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8987 a         9283 c         8500 g         8792 t
ORIGIN      
        1 ctaactttta tttttagtaa aattaaattg catatgctat cacattatgc aaaatagcca
       61 tcccgatgat gaatgaatgt aatagttggt aaacaacaga caaacatcat taacacagta
      121 acgattttgt tacgcagcat tgcagcagac tcttatcgtc atgcggtgcg gttatctctc
      181 tatggaatat tattcaagga tttttatgtc taaaaaatta ggttttgcgc tgtctggtat
      241 tatgttggca atggctgctg gtactgcatt tgctgctaac gatatggacg gtggtcagtt
      301 aaatattagc ggcctggtcg ttgataacac ctgtgaagct cgtgttgatg gcggtaacaa
      361 agatggtttg atcctgctgc aaaccgctac tgttgctgaa atcaccgatg gtgtactgga
      421 taccaccgta ggtgctaaag ctaagccatt cagcattact attgactgca gcaaagctaa
      481 cccggctccg ggtactactg caaaaatgac tttcggttcc gtgttcttta gcaatagcaa
      541 aggtactctg aataacgata tgtctatcaa taatccatct gatggtgtga atattgccct
      601 gcataatatt gaaggttcca ctattaagca ggttcaggtt aataacccgg gtgatgttta
      661 cagcaaaact ctggatgcaa cgtctaaaac tgcaacctac gattttaaag catcctatgt
      721 tcgtgctgat gcaagcaaag cagcaactgc cggttatgta aaaactaact cagcatatac
      781 cattacttac cagtaaatat cagccaccta aattgattgg gtgaatatat atagggaata
      841 acatcaaaca tatttatttt tgaacgttat tccctgtttt aattaactct gtgaggaacg
      901 gtttatgttt tttaatacta aacataccgc agcattgtgc ttagcgagct gtatggcttt
      961 tagctcatca gctattgcag acatcgtaat ttcaggtaca cgcgtagttt ataaaagcga
     1021 acaaaaaagc gtcaatgtgc gtctggaaaa taaaggcaat aacccattgc tggttcagag
     1081 ttggttagat actggagatg acaacgctga accaggcagt attactgtcc cctttaccgc
     1141 tacgccacca gtatcacgta ttgatgctaa acgtggccaa acaatcaaac tgatgtacac
     1201 cgccagtagt gctctgccaa aagacagaga aagtgtgttc tggtttaacg tactggaagt
     1261 tccaccaaaa cccgatgcag caaaagcagc aaatcaaagc ctgctacaac tggcatttcg
     1321 tacacgtata aaactgttct atcgcccgga aggattgagt ggaattccat ctgatgcgcc
     1381 attggctctg aagtggtcct gggcaacatc tgaaggcaaa gcggcattac gcgttgataa
     1441 ccctacgccc tactatgtct cttttagcag tggtgattta gaagccaacg gaaaacgcta
     1501 tcccctggat gtgaaaatga ttgcaccgtt cagtaacgac gtcatcaagg taaatgggat
     1561 gagtggcaga acaagctctg caaaagtgca ttttttcgcc attaatgatt ttggcggctc
     1621 tattgaaggc aacgccaacc tttaatctgg caaggatata ccacagcagg aagcagaacg
     1681 tgactattaa atccactaaa aaatatcata atcaccttac ccgcatcgcc acgttttgcg
     1741 cgctgctgta tagcaacagt gctttatgcg ctgaactcgt cgaatatgac catacatttc
     1801 tgatgggaaa ggatgcttca aatatcgacc ttagtcggta tacagaaggc aacccgacac
     1861 taccaggcat ttacgatgtc agtgtgtatg ttaacgatca acctatcatg agccaaagca
     1921 ttgcttttac tgttattgaa ggtaaaaaga acgctcaggc ctgcattacg cagaaaaatt
     1981 tattgcaatt tcatatcagt tcaccagaca agaatagcga aaaagccatt ttgcttaagc
     2041 gagacgaaga tcttggtgat tgccttaatc tggctgaaat gatccctcaa tcatccattc
     2101 gttacgatgt taacgatcaa cgcctggata tcgacgtccc ccaggcatgg attatgaaga
     2161 attatcaaaa ctatgttgat ccctctttgt gggaaaatgg cattaatgct gccatgcttt
     2221 cctacaacct caacggatat cacagtgaaa gtccaggcag aacaaatgac agtatctacg
     2281 cggcattcaa cggtgggata aacttaggtg cctggcggtt acgtgcctcg ggtaactaca
     2341 actggatgac caatgtccat agcgattacg attttcagaa tcgctattta caacgcgatc
     2401 ttgcgtcact acgttcacaa ctggttattg gtgaatctta cacaaccggc gaaaccttcg
     2461 attcagtcag tattcgcggt attcgcttat acagtgacag tcgaatgctg cctccggtat
     2521 tagccagttt tgcgccaatc attcatggcg tagcgaatac caatgccaaa gtaaccgtta
     2581 tgcaaaatgg ttataaaatc tatgaaacga cggtgccgcc aggggccttt gccattgatg
     2641 atcttagccc gtcaggttat ggcagtgatc tcattgtgac tatcgaagaa gctgatggca
     2701 caaaacggac gttctcccag ccattttcat ctgttgtgca aatgctgcgc cctggcgttg
     2761 gtcgctggga tattagcgcc ggtcaggttt taaaagacag tatccaggat gagcctaatt
     2821 tatttcaagc cagctactac tatggtttga ataactacct gacaggatat accggtattc
     2881 agctaactga taataactat accgccggat tgctggggct tggtatgaat acccccgtgg
     2941 gggctttttc tgtcgatgtt actcactcaa atgtcagcat tccggacgat aaaacctatc
     3001 aaggacaaag ctaccgtatt tcctggaaca agttatttga aaataccagt acatcattaa
     3061 atatcgcggc ctatcgctac tccacccagc attatctggg cctcaatgat gcgctgacgc
     3121 tgattgatga agtagaacat cctgagcaag atcttgagcc taaatccatg cgcaattact
     3181 cgcgcatgaa aaatcaggtc acggtcagta tcaaccaacc gctgaaattt gagaaaaagg
     3241 attacggttc attttatctc tcgggaagct ggtcagatta ctgggcctcc gggcaaaaca
     3301 gtactaatta ctctattggt tatagcaata gtgcgtcctg gggcagttac agtatcagtg
     3361 ctcaacgttc gttgaatgaa gatggtcaaa ccgacgatag catttatctc agcttcacga
     3421 ttccaataga aaatttactg ggtacggaac accgcagctc cggcttccag agtattgata
     3481 cacaattaaa cagcgacttt aagggtaata accaactcaa tatcagcagt agtggttaca
     3541 gcgataccaa tcgcatcagc tacagcgtaa acacgggtta tatgatgaat aaatccagtg
     3601 atgacctgag ttatatcggc ggttatgcca gttatgaatc gccgtgggga actctgtcag
     3661 gttcagcctc agcaagcagt gataatagtc gccaattctc actcaatact gatggtggat
     3721 ttgtgttaca tagcggcggg ctgacgttca gtaatgacag ttttagtgat tccgacactc
     3781 tggctgtcat tcaggcgcca ggagcaaaag gcgctcgtat taactacgga aacagcaccg
     3841 tagaccgctg gggttatggt gtgaccagcg ccctttcgcc ataccatgaa aaccgtatcg
     3901 cactggacat taacgatctg gaaaacgatg ttgaattgaa aagcaccagt actgtcgccg
     3961 tcccgcgcca gggagccgtc gtctttgctg attttgaaac agttcaggga caatcggcaa
     4021 ttatgaatat cgtgcgaagc gatggtaaaa atattccatt tgcagcggat atttatgatg
     4081 aacaaaacaa tatcatcggc aacgtaggtc agggtggtca ggcctttgtt cgcggtatcg
     4141 ggcaggaagg aaatatacga attacctgga ttgaagaggg caaaccagtc agttgttttg
     4201 ctcattacca acaaaacacg acatcagaaa aaatagcgca atctattatt ctgaacggac
     4261 ttcgttgtca gatacagtaa ccacaaggga cttccaatga taaaaaccac accacataaa
     4321 atagcaatac tcatggggct attattatcg ccatctgtat ttgcaacgga tgttaatgtc
     4381 gattttaccg ctaccgtgaa agcgacaaca tgtaacatca ccctgacatc gctgaatggt
     4441 tcaaacgtcg ttgataatgg caatgatagt tataccctga taatccccaa gatgggactg
     4501 gataagatag tcaataaagc gtcacaatct gaggccaact tcaaattagt cgccagtggg
     4561 tgtagcagtg gaattagctg gattgatacc acaatgactg gaaatcagtc tggtagtaca
     4621 tcgctgatta ttccacaatc ttctgactcc tcctcaacaa ccagtaatat tggtatggga
     4681 tttaaaagat tagagacttc gggcgatacg tttctgaaac ccaatagcgc ggaatacatc
     4741 cgctggaaaa ccacagaaat tagcaccagt ggtctggaaa tgactgtcgc actgcgcgaa
     4801 acaaccccag gacagggcat acccgggaaa tttcgcgcac aggcaacgtt caatttcatc
     4861 tatcaataac aaggatgaca atgatgatat taaaaaaatt atgttatgga ttatcgggta
     4921 gtctatttct ggcttcatca ctgttaacca tttcctcaca tgcggccact gatagtattg
     4981 gcctgactgt tactacagac atagaaatgg gtacctgtac atcaacgttg actgatgacg
     5041 gtacagctaa actgtccacc atcaatttcg gtgatgtata tatttctgaa attaatgccg
     5101 aaactaaaat taaaaccttc aaattgcagt tcaaagactg tgcggggatt cccgggaaaa
     5161 aggccaaaat aaagttgacc ccacgagcct tatgcgaagg taattctaat aacggccctg
     5221 gatttgctaa cgcatcaacg gcaacagcaa aagcagcggc agtcgccgtt gaggtctgga
     5281 gtacgtccac accaggtaaa aacggcgcaa aacaattcag ttgcgtcacc cccgcaacag
     5341 aagaagtcag tattgctggt gcgacgggag gtgatgttgt cgattacccg atgagtgctg
     5401 ttttggtggt cgcgaaagat aaaaccgtta cagatgtcac cgcgggtgat ttcaccgctc
     5461 cggcaacatt tactgtgact tataactaac aggcagaccg attcaggaac taaatatgcg
     5521 ccccactcaa cgtaatctga tgaaaaaatc aattctgctt ctgtctttgc tattcagcac
     5581 cactttccct gtggttgcag gacaggatgt tgacattgtt gcaaatataa aaaataacac
     5641 ctgccaaagt ggaatcagca acaacggaaa tattgattta ggtgtcgttg gcgtgggcga
     5701 ttttaccgga aacatttcag ccgaaaatta tcaccccggt ggaaaagagt tcactattac
     5761 agtacaggac tgcacacttc agggcacagg gaatgtgtta aatcagttac atattgattt
     5821 tcgcgcactc agtggcgtaa tggcgacagg ttccagtcaa atattcgcca atgaagacaa
     5881 caccggggct aaaaatgttg gggtagttat attctctatt caggacccgg caaatacctt
     5941 caacgttctt ggcgtaactg gtagctcacg ttccgtttat ccggtcatgt catcagcact
     6001 aaacaattca tcctggaaat ttagcactcg aatgcaaaaa atcgatccag cgctgaatgt
     6061 cacctctggc caacttataa gccatgtgtt agtggatatt tactacgaat aacagacaaa
     6121 gtgctgcaag cattatcttg cagcatgaca atggagtaaa aatgaagata ttcttcagat
     6181 actttctttt tttagcacta tgctcttgtt gttatacagc aagtgccggc accgatgata
     6241 acgtcagcta tcttgttggg aatgattacg gagtgggtcc gggagaccaa aagtggagag
     6301 aaacaggccc cagtgcagac attactgtca aattcaggta ttcaacaagc acaaataact
     6361 tagtttttta taaaccaacc caactcggac caacaggtgt gtcattatac tggtctcagt
     6421 taaatagcgc ttcaggtgga ggttaccttt actgcaacag aagtaacact gcaagcggtg
     6481 agcctatggc aattgaaaat caaatggttg attcgggtaa acgttatggc gggcataaac
     6541 tatttgaaac atctgtccca ggcctgtatt atacattaaa gatatcaaac atttggtcat
     6601 catacaccac aaccaatatc agttcatcag gtatgtatat cggcgattct ccaactcaat
     6661 attttcggtg gatccccgaa agtgaagcca ctctatattg gtcttgtaac aatgcaaact
     6721 catctaataa atactgggcc gtaggtggag tttggcagtc gctcacggtg gaattttata
     6781 ccgataccaa ctttgacccc acaaagacaa acaaggtaac attatctaag ccgggtggtt
     6841 atctctactc attcaaagga tatggtcttg gtggcggtat caacgatcac agctatttcc
     6901 ttaaaataga ttttgacctg gataatatga cgttatccaa cccaacgtgt tttactgctg
     6961 cacttactgg cccatctgta agcggttcaa cagttaaaat gggagattat accccagcgc
     7021 aaataaaaaa tggcgcaaca gcggttcctt ttgatatcac gctacagaac tgtattcgtg
     7081 ttcgcaatat agaaacaaaa cttatgtcaa acaaggtggg cagtgtaagc aaagaacttc
     7141 tggctaacac actcacagga aataacgcag caaaaggtgt tggtgtactg attgaaggtt
     7201 taaaaaacac taaaagtgca caaatggtac tgaaaccaaa tgattcaacg tctgtttata
     7261 aagattacga aacagaagat gacactaccg gaggaattta cccggataaa ggaaacggta
     7321 cctcacaacc tcttcatttc caggctacat taaaacaaga cggtaatatt gctatagaac
     7381 cgggggattt taaagccacc agcacttttc aggttaccta cccctgataa cactattgca
     7441 ccgccagaca atggcggtgc gatacggata aatcattgcc cccaccaatg tgactgataa
     7501 aatgcgagac agcacaacat catttatcag gatacgctat gaaaccaacc acgattgctt
     7561 cattgcagaa atgcaagcag gacaaaaaac gtttcgcaac catcaccgct tacgactata
     7621 gcttcgcaaa attgtttgcc gaagaaggac ttaacgtcat gctggtaggc gattcactgg
     7681 gcatgacggt tcaggggcat gactccacgc taccggttac cgttgccgat atcgcctatc
     7741 acaccgccgc tgtgcgtcgc ggcgcgccaa actgccttct gctggctgac ctgccattta
     7801 tggcgtatgc cacgccggaa caagcctttg aaaacgccgc aacggtaatg cgtgccggag
     7861 ccaacatggt caaaattgaa ggcggtgagt ggctggtgga aaccgtacaa atgctgaccg
     7921 aacgtgccgt tccagtttgt ggtcatttag gcctaacccc acagtcagtg aatattttcg
     7981 gtggctacaa agtccagggg cgcggcgatg aagcgggcga tcaactgctc agcgatgcat
     8041 tggcattaga agctgctggg gcacagctgc tggtgctgga atgcgtgccg gttgaactgg
     8101 caaaacgtat taccgaagca ctggcgatcc cggttattgg cattggcgca ggcaacgtca
     8161 ctgacgggca gatcctcgtg atgcacgacg cgttcggcat taccggcggc cacattccta
     8221 aattcgctaa aaatttcctc gccgaaacgg gcgacatccg cgcggctgtg cggcagtata
     8281 tggctgaagt ggagtccggc gtttacccgg gcgaagaaca cagtttccat taaggagtga
     8341 cgttgtgtta attatcgaaa ccctgccgct gctgcgtcag caaattcgcc gcctgcgtat
     8401 ggaaggtaag cgcgtggcgc tggtgcccac catgggtaac ctgcacgatg gccatatgaa
     8461 gctggtcgac gaagccaaag cccgcgccga tgtggtagtg gtcagtattt tcgttaaccc
     8521 gatgcagttt gaccgcccgg aagatctggc acgttatcca cggaccttgc aagaggactg
     8581 cgaaaagcta aacaaacgta aagtggattt agttttcgcc ccttcggtaa aagagatcta
     8641 cccgaacggt acagaaaccc acacctatgt tgatgttcct ggtctttcca ccatgctgga
     8701 aggtgccagc cgtccgggac attttcgcgg cgtttcgacg attgtcagca agctgttcaa
     8761 cctggtccag ccggacatcg cctgcttcgg tgaaaaagat tttcagcaac tggcgctgat
     8821 ccgcaaaatg gttgctgata tgggctttga tattgagatt gtcggtgtgc caattatgcg
     8881 cgccaaagac ggtctggcgc tgagttcccg taacggttat ctgacggcgg aacaacgcaa
     8941 aattgcgccc ggtctgtaca aagttttaag ttcgattgcc gacaaattgc aggctggcga
     9001 acgggatctc gatgaaatta ttactattgc cgggcaagaa ctgaatgaaa aaggcttccg
     9061 cgccgatgat attcagattc gtgatgccga caccttgctg gaggtctctg aaaacagcaa
     9121 acgtgcagtg attctggtag ccgcctggct tggcgatgcc cgcctgatcg acaacaaaat
     9181 cgtcgaactg gcgtaatcct tatctggcgc tacggctgat ggcgccagtt attcattgcc
     9241 acgttatctg taggcaatta ttcatatcgt agacaactta gttagagtta attagcttat
     9301 caatctctgc aagagataaa gctgtcacct ctgcaacgtc ttcccgagac attcctttac
     9361 tcagaagacg ctgggccagt cttaccttct ggagtccctc tttatatccc ttctgaaatc
     9421 ccttctcttc aaaccactgc gccaacgtct gcctggtttc tctccgtgtc tttatttcca
     9481 tatgttccct taattatctg gtcagttaac aataacaatt atctggctat aactgataat
     9541 gcagataact tagttaatca gcttatcaac ctcagcaaga ggtaaatttg ccatctctgc
     9601 aacgtcttcc cgagacatcc ctttactcag aaaacgcagg gcgtattcct gtcttacttc
     9661 ctgtctaacc tcttgtatta cctcctgtct tacctcctgt cttacctcct gtcttacctc
     9721 ctgccttacc tcctgccttt cctcctgtct tcccttctct tcaaaccact gcgccagcgt
     9781 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     9841 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     9901 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     9961 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
    10021 cggtgtgatg gtgatatcca ccagcgggaa agggttgttg taaacgcgtc gcgccagctc
    10081 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
    10141 ataaaacaga atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
    10201 catggcggca atggcatagc gcatcatgcg aaaggtcatt ttcttatccg gcttgctttg
    10261 atgttcaatc accacatgca aataaccggg attaccctgc atttgcacgg aataaagcac
    10321 gtcagtgctg tgccctttca ggctttcttc aataaaactc cccgactcaa gatgcagcgt
    10381 gtcgaggtca cacagttcgc gtagttccgc tggcaaatgg atatccagaa agtcacgagc
    10441 cgtttccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
    10501 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgcgc
    10561 cgtcgtcagc cgcctcttta ttgtttaccg agcagcgttc agagaaattt caattggtta
    10621 caggcgttac atggcacgct tcggcacggt acgcaaactg ctgatgcaaa tgccgtcaga
    10681 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
    10741 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
    10801 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
    10861 attgaccagg attttcttga cgcagctggt attctcgaaa acgaagccat tgatatctgg
    10921 aatgtgacca acggcaagcg tttctccacc tatgccatcg cggcagaacg cggttcgaga
    10981 attatttccg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
    11041 gccagcttcg ttactatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
    11101 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcctga
    11161 taatcacccc tgcggctggt tactcaccag ccgcgacatc gtctcgagcg aatccgttct
    11221 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
    11281 ggttgtcacc gccaggtgaa atccggcttc ttttgctgcc ttcacggctt tgtcattaaa
    11341 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
    11401 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagcaata tgggtcggcg
    11461 ataaccatcc acccgatgca aaaaatgggt atgtgactgg aaatcaaata catcgcgaat
    11521 ttcgttaagt tcagaaacgc tcataaattg cagcgatttt gggtcccact tctgcgggtg
    11581 acgtttgatg cgcgaggtaa caataaacgc cgtcgccttc atgccatatt gtttcaacac
    11641 aggatacgca tagcggctca ccgatttgag gccatcatca aaggtaatca ccaccgctcg
    11701 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcaccatac tcagtgtcgt
    11761 gtatcccctg tcgcgcagcc aggccatctg gttattgaaa gcgcgtaccg aggtggtcgt
    11821 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aggttagcac
    11881 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
    11941 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
    12001 caaattgtcc gccagtaccc caaacggcgc actgcccgca ctcggcgcgt tatagaccgg
    12061 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
    12121 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatga cctttatcga taaacccttt
    12181 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
    12241 cccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
    12301 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacgcttg cggtaaacag
    12361 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
    12421 cttttattgt agcaaaagcg tcatcagaat cgcgggttaa tactcataca aattatgttt
    12481 gaggacgaaa ggcggatttt tttgttgctg gtgccaaaga ctactcacct ccggcgcacc
    12541 cagttcgaca atacgctcgc gaaactctgc gctggataac gtttcacggt agaccagcat
    12601 ctgctcaatt aacggtaacg tgacgccaga aatcacttcg caacgggaat gtttatggct
    12661 taatagcgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
    12721 ttcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
    12781 taacccacgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
    12841 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
    12901 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
    12961 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
    13021 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtaca
    13081 ccgaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
    13141 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
    13201 agtgagtaaa agaccccgcc caaatacgta agtggcgtta acacaaaggt tggcaccagg
    13261 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaacaac
    13321 accgccgtga gcaccagcgt taaagcgaca aatacccacg aatgcacctg aaacggcaca
    13381 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
    13441 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
    13501 ttacgctgaa acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatg
    13561 accgacatca tgatcagccc cggtacaatg aactgcatgt agctgaagcc atgcatatcg
    13621 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgacc
    13681 ggtggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
    13741 ctttttagtg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
    13801 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
    13861 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcaga acttcaactt
    13921 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacgggcttt
    13981 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
    14041 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
    14101 cttcttccag gtagtgtgtg gtgagaatga tagtggtgcc tttgtcgttt aaatccttca
    14161 aaaagcccca cattgagcgg cgaagttcga tatccacgcc tgcggtcggt tcgtcgagaa
    14221 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
    14281 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatact
    14341 tttcgctgcg aatgtacgct tctttgcgct ccacaccgta gtaccctgcc tgattcacca
    14401 caatttgctg cacggtttca aacgggttga agttaaattc ctgtggcacc agacccaact
    14461 gacgtttagc gtttacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
    14521 aggttttatt taccagagag ctgataatac cgatagtggt cgattttccg gccccgttcg
    14581 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atcgattcca cgaagcgcct
    14641 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
    14701 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
    14761 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
    14821 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
    14881 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
    14941 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
    15001 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
    15061 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
    15121 cgcagttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
    15181 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
    15241 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
    15301 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
    15361 cttgctgcgt gatctggatg tcacagcgac taaccgcgaa acccttgagc aacgttaccg
    15421 tcacgggatc tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
    15481 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccgatatac
    15541 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
    15601 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
    15661 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
    15721 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
    15781 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
    15841 gaggcggtca taaaatcgac ttcatgagat acctgaactt cacggcacag gtccgccata
    15901 aacataaatg agccacgcag cagaccaacc agcaccatat cgctgccact gtctttgtaa
    15961 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
    16021 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
    16081 acgggcaggt ttcagcaccg caacaaagcg caagacacgc agtataccag taaaactatt
    16141 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac gatcacacat gttaaaacct
    16201 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
    16261 aataccggct cgcggcgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
    16321 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
    16381 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
    16441 ctttggctgt acgcagccct gctgctcggc actatgattt ggggcgtctg ggaagttggt
    16501 ttcgacttct gggcgctgac tccgcgcagc gacattctgg tcttcttcgg catctggctg
    16561 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
    16621 gtggtggcac tgctgattag cggtggtatc ctggcctggg ccggatttaa cgatccgcag
    16681 gaaatcagcg gcaccttaag cgccgatacc acgccagcag aagccatctc ccccgttgcc
    16741 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
    16801 caaattaacg ctgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
    16861 gtgaagcagc cgaacgatcc aggtgaaatc accaatgaag tgacgccaat taaagtgggc
    16921 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
    16981 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtccttcca gcatgtaacc
    17041 tgccgtggcg tctcttatca tgaagccaaa gcagaaactg cttcgccgga agtgatggcg
    17101 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gtctgattgc aattaacgct
    17161 gaaaacggca agctgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    17221 atgccggaca ccaaaccggg tctgtatgaa ccgacttcgc cgccgattat caccgataaa
    17281 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgtgaaac atctggcgtg
    17341 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc tggtgcgaaa
    17401 gatcctaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcgtgg
    17461 gcaccagcgg cctatgacgc gaagctggat ctggtgtatc tgccgatggg cgtgaccacg
    17521 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    17581 gcgctgaatg ccacaaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    17641 tgggatatgg atcttccggc acagccgacg ctggcggata tcaccgttaa tggtcagaaa
    17701 gtgccagtta tttatgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgtaat
    17761 ggcgaactgg tggttccggc accggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    17821 tacgtaaccc caactcaacc gttttctgaa ctgagcttcc gtccgacgaa aaatttgagc
    17881 ggtgcggata tgtggggagc caccatgttt gaccagctgg tgtgccgcgt gatgttccac
    17941 cagatgcgct atgaaggcat tttcaccccg ccatctgaac agggtacgct ggtcttccct
    18001 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccgaatcg tgaagtggca
    18061 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tcctggcaac
    18121 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccgcag
    18181 tacggtgtac cgtatggcgt cacgctcaat ccgttcctct caccgtttgg tctgccgtgt
    18241 aaacagccag catggggtta tatctcggcg ctggatctga aaaccaatga agtggtgtgg
    18301 aagaaacgta ttggtacgcc gcaggacagt atgccattcc cgatgccggt acctgtgccg
    18361 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    18421 atcgccgcta cggcagataa ctacctgcgc gcttataaca tgagcaacgg tgaaaaactg
    18481 tggcaaggtc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggt
    18541 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    18601 tatattgtgg cttatgcgtt gccggatgat gtgaagtaag gcttgctcag attgctgaca
    18661 acgtgcgatt tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaacattt
    18721 gcaaattcaa tacattgcgc gtaggcctga taagcgtagc gcatcaggca gttttgcgtt
    18781 tgtcatcagt ttcatgcccg gagagatccg ggcatatttc cgaatacggt ctttttatac
    18841 cgtaaaccct aacatcatcc ccgtatcttc atgctccagc aaatggcagt gcgccatata
    18901 agcatgttct ttcggtgcat cgtgattaaa cttcaccagc acttcgctga cattaccttc
    18961 taccttaacg gtatctttcc agcctgcgcg atgcgtcgct ggcggtttgc cattttctga
    19021 caagatacgg aactgcgtac cgtggatatg gaacggatgc agcatcatgt cgcccacgcc
    19081 agagataacc caacgttcgt attgcccttt cgccgccgca aacatcggct tgttcatatc
    19141 aaacgcctga ccgttgattt tattggcatg gtggaaatcg aacttcccgc cgtggttcat
    19201 atgattcata ttgccgtgcc ccatatggcc catcatctgg ctgtgatcca tcccggccat
    19261 cgcctgatcg ccatatttct ccatcagcat ctgcatcccc atcatatcga gcattgggtc
    19321 catagaaagt tgcagcttgc gtaccgtcag cccttccagc gaaggtaacg caggcaggct
    19381 acttaatgtg tctggcaaag caccagaggc actaatggca atcggctgaa tccgcattac
    19441 cggatgcggc ttgtcaaacg gcgcaatcgc catccccatc tggctgaccg gcagcgtcac
    19501 caggtcaaag ggtttgttgt cgttaacctc caccagcact tcaaaacgct cgcccatcag
    19561 cactggcagc tcgttcacct tcaccggttc aggtagcaga ccgccgtcgc tggcaatcac
    19621 ataaagcggg cgattgtcgc tggtggcgaa attgagcgag cgggcattac agccattgag
    19681 caaacgcagg cgcagccagc cacgcggggc agcgtgttgt gggtagatta caccgttggt
    19741 cagcaacgta tcgccaaacc aacccacagc ggcggtcatc acatccagtt gataatcaat
    19801 ctgcccgtca gcgttaaatt ttttatcctg aacaataacc ggaacatcat caatacccca
    19861 ctgttttggc agcattaatt tcaggatctc gtcatcttca atcaccacca aaccagccag
    19921 ccccatcgcc acctgacgtc cggtcttgcc atgttgatgt ggatggaacc agcaggtagc
    19981 ggcaggttga tcaacgttca acgtcaccga gcgcttgcca cctggcggaa taattccctg
    20041 cgggccgccg tccacttcac ccggtacttc cagcccgtgc cagtgcaacg tcgtctcttc
    20101 cgtcagttgg ttgtagatat caaccgttac cgctttgccg cgctgtaatt tcaccgccgg
    20161 ccccagcaga ttgccgttat agccccaggt agttgcggtt ttcccgccaa aggtggactg
    20221 acctgcgcca atagttaact gaatgcgatt acgggcatcg gtcgtgagca aatcagggat
    20281 cggtaacgtt gggcgttccg ccgcaaatac tgcgcggctc cacagcggca aagccgaagc
    20341 cacgcccagc gcgacggaat attttaagaa atcacgacgt tgcatagtta tttccttatt
    20401 cttaagcagg cgaaacaaaa tcaaacgttg agcatagtcc ttccccttac gggaaggtca
    20461 agccgcagac acaatttaat cgacaataat ggtcgtcgcc tcgctcgcag tgtgctaacg
    20521 tttatcttct ttaagcccct ggtagaagca atgaagacgt ttttcagaac agtgttattc
    20581 ggcagcctga tggccgtctg cgcaaacagt tacgcgctca gcgagtctga agccgaagat
    20641 atggccgatt taacggcagt ttttgttttt ctgaagaacg attgtggtta ccagaactta
    20701 cctaacgggc aaattcgtcg cgcactggtc ttttttgccc agcaaaacca gtgggatctc
    20761 agtaattacg acaccttcga catgaaagcc ctcggtgaag acagctaccg cgatctcagc
    20821 ggcattggca ttcccgtcgc taaaaagtgc aaagccctgg cccgcgattc cttaagcctg
    20881 cttgcctacg tcaaataatc cctgatacct ttttgtagaa atattgaccg tgcatccgcg
    20941 gtcaatgtta gctattatgt tgcgcccttt ttttacgggt gttaacaaag gaggtatcaa
    21001 cccatggccg aaaaaaaaca gtggcatgaa acgctacacg accagtttgg gcagtacttt
    21061 gcggtagata acgttctgta tcatgaaaag accgatcacc aggatctgat catttttgag
    21121 aacgccgcat ttggtcgcgt aatggcgctg gatggcgtag tacaaaccac cgagcgcgac
    21181 gagtttatct atcatgagat gatgacccat gttccgctac tggcccacgg tcacgcgaaa
    21241 catgtgctga ttatcggcgg cggcgacggt gccatgctgc gtgaagtaac ccgacataaa
    21301 aacgttgagt caatcacgat ggtggaaatc gatgcgggtg tcgtgtcgtt ctgccgtcag
    21361 tatctaccca accataacgc cggtagctac gacgatccgc gctttaaact ggtgatcgac
    21421 gatggcgtca atttcgttaa tcaaaccagc cagacctttg atgtcattat ttccgactgc
    21481 accgatccta tcggtcccgg cgaaagtctg ttcacctcag cattttatga aggctgcaaa
    21541 cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg gcgtctgctt tttacagcag
    21601 gaagaggcca tcgacagcca tcgcaaactc agccattact tcagcgatgt tggcttttat
    21661 caggcggcga tcccgaccta ttacggcggc atcatgacct ttgcatgggc gacagataac
    21721 gacgccttac gccatctctc aaccgaaatt attcaggcgc gttttctcgc ttctggcctg
    21781 aaatgccgtt attacaatcc ggcagtccat acggcagctt ttgccttacc ccagtatctg
    21841 caagatgcac tggcttcaca gccgtcctaa ggagaagata agaaattgaa aaaactgaaa
    21901 ctgcatggct ttaataatct gaccaaaagt ctgagttttt gtatttacga tatctgctac
    21961 gccaaaacta ccgaagagcg cgacggttat attgcttata tcgatgaact ctataatgcc
    22021 aaccgtctga ccgaaatcct gtcagaaacc tgttccatta tcggggccaa tattcttaac
    22081 atcgcccgcc aggattacga gccacaaggt gccagcgtca ctattctggt gagtgaagaa
    22141 ccggtcgacc cgaaacttat cgacaaaaca gaacaccccg gcccactgcc agaaacggtc
    22201 gttgcccatc tcgataaaag tcatatttgc gtacatacct acccggaaag tcatcctgaa
    22261 ggcggtttat gtaccttccg cgccgatatt gaagtctcta cctgcggcgt gatttctccg
    22321 ctgaaggcgc tgaattacct gatccaccag cttgagtccg atattgtgac tattgattat
    22381 cgcgtgcgcg gttttacccg cgacattaac ggtatgaagc actttatcga ccatgagatt
    22441 aattcgattc agaactttat gtctgacgat atgaaggcgc tgtatgacat ggtggatgta
    22501 aacgtctatc aggaaaatat cttccatacc aagatgttgc ttaaagagtt cgaccttaag
    22561 cactacatgt tccacaccaa accggaagat ttaaccgaca gcgagcgcca ggaaattacc
    22621 actgcgctgt ggaaagaaat gcgcgagatt tattacgggc gcaatatgcc cgctgtttaa
    22681 cgtctcaggc ggagcacgga ggctccgcct gatttactta cttctgctgc acgaaattac
    22741 ggtaagccgc caccacctga agaaaatcct caacgccgca cagcgacaag ctctcttcat
    22801 cgtagtagtt catcccctct tccatttcat cgccagcgaa ttccagttga ttggcgcgaa
    22861 ccatcacctc ttcaccatcc atccatagcg tgtattcatg ccctgcccgt tgccaggaac
    22921 gttcgctacc tttcaatgcg tgcgccgctt gttctacttc atcaagcaag gccaggtttt
    22981 ctttcacctc ttcattaaac cagtgcccga ccacttcatg ccccatggac atgcgcacct
    23041 ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat agtgtgttcc tctgctcctg
    23101 gcaatgcgcc gaaatcgcat tgcgccattg atgtaattat cgcagcctta ccggagaaaa
    23161 acagcggaac ggcgatggtt tagaaataaa aaatgcccgg gaatctcatt caccgggcat
    23221 tgtgtcgttt atgcgcagcg cgtgcgctta cttattaaac cgcagtctgg aaaatcaccc
    23281 cgtccgcttt ttcggtgtac tgagaaagct ggtcgaagtt cagataacgg taagtatcaa
    23341 cggctgtttt atctacctgc gccacgtagg tctggtactc ttccggcgtc ggcagtttgc
    23401 caatcagcgc cgcaacagca gccagttccg cagaagccag gaagacattc gcgccagtac
    23461 ccaaacggtt cgggaagtta cgggtagagg tggaaaccac cgtcgcaccg tctgctacac
    23521 gcgcctggtt acccatacac agggaacagc cagggatctc gatacgagca ccactcttac
    23581 cgaagacgct gtagtagcct tcttcggtca gctgcgcagc gtccatacgc gttggcggtg
    23641 ccacccacag gcgggtcggc aactgacctt tgtgcgcatc cagcagttta ccagcagcac
    23701 ggaagtgacc gatgttagtc atgcaagaac cgataaatac ttcgtcgatc ttctcgccct
    23761 gtaccgcaga cagcggacgt gcgtcgtccg ggtcattcgg tgcacagagg attggctctt
    23821 tgatatccgc cagatcgatg tcgatcactg ccgcgtattc cgcatctgca tcggcttcca
    23881 gcagctcagg attcgccagc catttttcca tgccctgaat acgacgctcc agggtacgac
    23941 gatcgccgta accttccgcg atcatccact tcagcaggac gatgttagag ttcaggtatt
    24001 caatgatcgg ttctttgttc agcttgatgg tacaaccggc agcagaacgc tcggcagacg
    24061 catcggtcag ctcaaatgcc tgctcaactt tcagatccgg cagaccttca atttccagaa
    24121 tgcggccaga gaagatgttt ttcttacctt tcttctcaac ggtcagcaga ccttgtttga
    24181 ttgcatacag cgggatcgcg tgtaccagat cgcgcagggt gatacccggc tgcattttgc
    24241 ctttgaagcg caccagaacg gattctggca tatccagcgg cattacgcct gttgcggctg
    24301 caaatgccac cagaccagaa cccgccggga aagagatacc gatcgggaaa cgggtatggg
    24361 agtcaccacc ggtaccgaca gtatccggca gcagcatacg gttcagccag gagtggatta
    24421 cgccgtcacc cggacgcagc gacacaccgc cacggttcat aatgaagtcc ggcagcgtgt
    24481 ggtgcgtgtt aacgtcaact ggtttcggat acgccgcggt gtggcagaaa gactgcatca
    24541 ccaggtcagc cgagaagccc aggcacgcca ggtctttcag ttcatcacgg gtcatcgggc
    24601 cggtggtgtc ctgggaacct acagaagtca ttttcggttc acagtacgcg cccggacgaa
    24661 tgcctttcac gccacaggca cggcctacca ttttctgcgc cagcgagaag ccgcgatcgc
    24721 tctcagcgac atctttcgcc tgacggaaca catcgctgtg cggcaggcca agtgcttcac
    24781 gcgctttggt ggtcaggcca cgcccaataa tcagcggaat acggccgcca gcacgcactt
    24841 catcaatcag cacgtcggtt ttcagttcga aggtcgccag cagttcgccg gtttcgtggt
    24901 tacgcacttc acctttgtac gggtaaacgt caattacgtc gcccatgttc aggttagaga
    24961 cgtcgacttc gattggcagc gcacccgcgt cttccatcgt gttaaagaag atgggtgcaa
    25021 ttttaccgcc gaggcacaaa ccaccgccgc gtttgttcgg cacatgtgga atatcatcgc
    25081 ccataaacca cagaacggag ttagtggcgg atttacgcga tgaccccgta cccacaacgt
    25141 caccgacgta cgccagtggg aaacctttct gttgcagagc ttcgatttgc ttaatcggac
    25201 caacaacgcc tggctgatct ggctcaatac cttcacgggc gtttttcagc atcgccagcg
    25261 cgtgcagtgg gatatccggg cgtgaccacg catccggtgc cggagagagg tcatcggtgt
    25321 tggtttcgcc tgtgactttg aagacggtaa cggtcagttt ttcagccagc gccgggcgat
    25381 tcaggaacca ttcggcatcc gcccaggact gcattacctg cttcgcatat tcgttgcctg
    25441 ctttcgcttt ctcttctacg tcatagaagt tatcgaacat cagcagcgtg tgagaaagtg
    25501 ctttggcagc gatcggtgcc agtttggcat catccagcgc gtcgatcagc ggatgaatgt
    25561 tgtaaccacc ctgcatggtg cccagcagtt cgatggcttt ttccggagtc agcagagggg
    25621 atttggcttc gcctttagcg atagcagcca ggaagcctgc ttttacatag gcggcttcat
    25681 cgacgcctgg gggaacacgg ttggttaaca gatctaacag gaattcttct tcgcccgcgg
    25741 gcgggttttt cagcagctct acaagcgcgg ccatttggtt tgcatccagg ggtttgggcg
    25801 caatcccctc agcggcacgc tcagctacgt gcttacggta ttcttctagc acgacggttc
    25861 tcctcgctct cattgtcata gtgcggcagg cgattctctt cacgctcctg tgagacagca
    25921 gtttgtaggg taaatgccca gtaccgcaac gggcagaata gcaggatttt gacgatgtgt
    25981 taatctgttt acaaaaaagc aacataaaaa agtggctgaa tcgttaagga tggtctagag
    26041 attgtttccc tccgtaaaaa cttcgttaat tcgcatggtg ataatcacct ttctcaacga
    26101 atcccaagat tagtcaaaat ttaaactacc gcctctttat actcggattc acagcacctg
    26161 cgggtggcag ttcgccgacc attgcgattt ccttgagatc cgaattatga aaatgacttt
    26221 gccgtttaaa ccccatgtgc tggcgctaat ttgcagtgcc gggctttgtg ccgcctctac
    26281 cgggctatat ataaaaagcc gcacagtgga agcgcctgtg gaaccgcaat cgacacaact
    26341 ggctgcgcct gacatctccg cagttacgct tcctgcaacg gtttccgtgc ctcctgtaac
    26401 acctgccgtc gtcaaatccg cattcagcac tgcgcaaata gatcaatggg ttgcacctgt
    26461 cgcgctatac cccgactctc tgctttcaca agtgttaatg gcatcaacct atccggcaaa
    26521 cgttgctcaa gcagtgcaat ggtcgcacga taatccactt aaacaaggcg atgctgctat
    26581 tcaggcggta tctgaccagc cctgggacgc cagcgtcaaa tcactggtgg cctttccaca
    26641 gttgatggca ttgatgggcg aaaacccgca atgggtgcaa aacctgggcg atgcttttct
    26701 ggcgcagccg caggaggtga tggactcggt acagcgattg cggcaactgg cgcaacaaac
    26761 cggctcgctg aagtcatcaa ctgaacagaa agttattacc acaacgaaga aagctgtacc
    26821 ggtaaaacag acagtcacgg ctcccgtcat accatccaat accgtttcaa ctgccaaccc
    26881 tgtcattaca gagcctgcaa caaccgtcat ttccattgaa cccaccaatc ctgatgtggt
    26941 ctatattccc aactacaacc caaccgtggt ttacgggaac tgggccaata ctgcgtatcc
    27001 gccggtttat ctgccgccgc cagccggaga accgtttgtt gacagctttg tgcgcggatt
    27061 cggctatagc atgggcgttg ctaccacgta cgcgctattc agcagcatcg actgggatga
    27121 cgacgatcat gaccatcatc atcatgacga tgatgattat caccatcacg atggcggtca
    27181 tcgtgatggt aatggctggc aacacaatgg cgacaacatc aatatcgacg tcaacaattt
    27241 caaccgtatc accggtgaac atcttactga taagaatatg gcatggcggc acaatccaaa
    27301 ctaccgtaat ggtgtgccct atcatgatca ggatatggca aagcggtttc atcaaaccga
    27361 tgtcaacggc ggaatgagcg ccacgcagct acctgctcca acacgcgaca gccagcgtca
    27421 ggcggcagca agtcagtttc agcaacgaac acacagcgca ccagtcatta cacgagatac
    27481 ccaacgtcag gcagcggcac agcggtttaa tgaagctgaa aactatggga gctatgacga
    27541 cttccgcgac ttcagccgtc gccaaccact gacccagcaa caaaaggacg ccgctcgtca
    27601 gcgttatcag tcggcctctc ctgagcagcg ccaggcagtc cgcgagaaaa tgcagactaa
    27661 cccgcagatc cagcagcgaa gagaggcagc gcgtgagcgc attcagtctg caactcccga
    27721 acagcgccag gtgtttaagg aaaaagtaca gcagcgccca ctgaatcaac agcaacgtga
    27781 taacgcccgc cagcgtgttc aatcagcatc acctgagcaa cgtcaggttt ttcgggagaa
    27841 agttcaggag agccgcccgc aacgtctaaa cgacagtgac catactgtca ggctgaataa
    27901 cgagcaacgg tcagcagtac gcgaacgtct ctctgagcgc ggagcaaggc gactggaaag
    27961 gtaaattaca gacgtaaaaa aagcggcgtg gttagccgct tttttaattg ccagatgttc
    28021 cggcaaacga aaaattactt cttcttcgct ttcgggttcg gcaggtcggt aatgctacct
    28081 tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc atggatggtc
    28141 agtgcgatgt cttcagcgtc acaacccatt tcgattgcca ggccgatttc acccagcaac
    28201 tcgccgccgt tagtgccgac aatcgcacca ccgatcacac ggtgagattc tttgtcgaaa
    28261 atcagcttgg tcataccgtc tgcgcagtcg gaagcgatag cacgaccaga agcagcccac
    28321 gggaaggtgg cggtttcgta gctgatgcct ttctctttcg cttctttctc agtcagacct
    28381 acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg atcgaagtaa
    28441 tgttttttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc tttgtgtgcc
    28501 agcattggct gaccgacgat atcaccgata gcaaagatgt gcggtacgtt ggtacgcagc
    28561 tgtttgtcaa cgcggatgaa accacggtcg tcaacttcca cgccagcttt gcctgcgtcg
    28621 aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta acgctgcggt
    28681 tcagcgggtg cttttttgcc ttccatcgtc acataaatgc cgtcttcttt cgcttcaacg
    28741 gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt ggtaaagact
    28801 ttaacgatgt ctttatcagc tgccgggatg acctggtcga acatttcaac cacgtcaatc
    28861 tgtgaaccca gcgcgtggta tacggtgccc atttccagac cgatgatacc gccacccatt
    28921 accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtggagtc ccagatacgc
    28981 ggatcttcat gcggaataaa cggcagttga atcgggcgag aacccgctgc aatgatcgcg
    29041 ttgtcgaagt tgatcacggt tttaccgttc tcaccttcaa cttccagggt gttagccccg
    29101 gtaaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat accagccaga
    29161 ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc gatatcggtt
    29221 ttcggttcgc caaagacgat accgtgttca gccagcgctt tggcttcttc gataactttt
    29281 gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac accgccaagg
    29341 gtgttgtagc gttctacgat tacggtttcc agacctaaat cagcgcaacg gaaggcagcg
    29401 gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc agtactcatc
    29461 atgacctcta tatatttatc tccggcggtc atacccgtcg tctttcaggc cgcagcggcg
    29521 ttagcttcac gccctcaccc cagtcactta cttatgtaag ctcctgggga ttccggcgct
    29581 tgctaccttg ctgcacccag aaatccatag ggtatgtgaa gtaataccct aaccaccacc
    29641 gggtcgttct atccgtcggt attttacaaa attgttaaca atttttaaac aacaaacggc
    29701 aaccgatttg tctattcgct aataacctca atacattcat gagattacca gaaaaaagcc
    29761 ggccgttggg ccggctcttt tacttacatc accagacggc gaatgtcaga cagcgtgttg
    29821 ttaatgatgg taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg gtcgaaggag
    29881 agagaaatcg gcagcatcag acgcggcacg aactctttac cattccacac cggctccatc
    29941 gcggacttgg aaacgccgag gatagccact tccggcgcgt tcacaatcgg cgcgaagtgg
    30001 gtagtaccca ggccgccgat gctggagatg gtgaagcaac cgccctgcat ttcgcccgca
    30061 gtcagcttac cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga cagctcgatg
    30121 atgcctttct tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg ggtatccacc
    30181 gccacaccga tgttgatgta tttcttcagg gtcagacgct gaccgtcttc cgacagcgaa
    30241 ctgttgaagc gaggcatctg ctcaagagct gcagcaacgg ctttcatgat gaagacaacc
    30301 ggggtgatct tcacatccag cttacgtttc gccgcttctt cgttctgctg tttacggaac
    30361 gcttccagct cggtgatatc ggttttgtcg aagtgggtaa catgcgggat catcacccag
    30421 ttacggctca ggttcgcacc agagattttc tggatacggc ccagttccac ttcttcgatt
    30481 tcaccaaact tgctgaagtc caccttcggc cacggcagca tgcccgggat accgccgcca
    30541 gtcgccgccg gagccgcttc tgcacgtttg atagcttctt tcacgtaagc ctgaacgtct
    30601 tcgcgcagga tacgaccttt acggccagtg cccttcactt tcgccaggtt aacgccaaat
    30661 tcgcgtgcca gacggcggat cagcggagtc gcgtgaacgt aagcgtcgtt ttcagcaaat
    30721 tcagatttgc cttcggcttt cgcagccgga gctgctgctg gagcttcagc ttttgctgcc
    30781 ggagccggcg ctgccgcttc ctgtttcgca ggagctgccg caggcgctgc gccttcaact
    30841 tcgaagatca taatcagcga gccagttttc actttatcgc caacgttgac tttcagttcc
    30901 ttcacgacgc ctgcaaacgg agccggaact tccatagacg ctttatcgcc ttctacggtg
    30961 atcagtgact gttcagcggc aactttgtcg cccactttca ccatcacttc ggtcacttca
    31021 acttcgtcac cgccgatatc cggaacgtta acgtctttca cgccagccgc tggtgcagag
    31081 gccgctgccg gagccgcttc ctgtttagcc gccggagctg ccgcgcctgc ttcacccgcg
    31141 acttcgaaga ccataatcag cgagccggta gacactttgt cacccacgtt cactttgatc
    31201 tctttcacgg tgccagcaaa cggagccgga acttccatag aagctttatc gccttctacg
    31261 gtgatcagcg actgttcagc ttcaacttta tcgccaactt tcaccaggat ttcggtcact
    31321 tcaacttcgt cgctgccgat atccggaacg ttaacgtctt ttgccgctgc agccgctgga
    31381 gctgctgccg gagcggcttc tttcttctct tctgcctgag caggtgcagc gtctgctgca
    31441 ccgtcggcgg aatcgaaaat cataatcagt gcgccggtct gggttttatc gccaacagag
    31501 actttgatct ctttaacgat acccgcctgc ggagacggaa cttccataga ggctttgtcg
    31561 ccttctacgg tgatcagcga ctgttcggct tcaactttgt cgcccacttt gaccaggatc
    31621 tcggtgattt caacttcatc agccccgatg tccggtactt tgatttcgat agccattatt
    31681 cttttacctc ttacgccaga cgcgggttaa ctttatctgc atcgatgttg aatttggcga
    31741 ttgcgtcagc aaccactttc ttatcgattt cgccacgttt agccagttcg cccagcgccg
    31801 caaccacgac ataagaagca tcaacctcga agtggtgacg caggttctca cggctgtcgg
    31861 aacgaccgaa gccatcagta cccagtacgc ggtagtcgtc agccggtacg taagtacgga
    31921 cttgctcagc gaacagtttc atatagtcgg tagatgccac tgccggagcg tcgttcatca
    31981 cctgagcgat atacggaacg cgcggagttt ccagcgggtg cagcatgttc cagcgttcac
    32041 aatcctgacc atcacgcgcc agttcggtga aggaagtcac gctataaacg tcagaaccta
    32101 cgccgtaatc tttcgccagg atctctgctg cttcacggac gtgacgcagg atagaaccgg
    32161 agcccagcag ctgaacttta cctttgctac cttcaatggt ttcgagtttg tagatacctt
    32221 tacggatacc ttcctcagca ccttccggca ttgctggcat gtggtagttt tcgttcagcg
    32281 tagtgatgta gtagtaaacg ttctcttgtt tctcaccgta catacgctcc agaccgtcat
    32341 gcataatgac agcaacttcg taagcgtaag ccgggtcgta agagatacag ttcgggatag
    32401 tcagcgactg aatgtggctg tgaccatctt cgtgctgcag accttcgccg ttcagggtgg
    32461 tacgaccgga agtaccgccg atcaggaagc cacgcgcttg ctggtcacca gccgcccagc
    32521 acagatcgcc gatacgctgg aagccgaaca tcgagtaata gatgtagaac ggaatcatcg
    32581 gcagattgtt ggtgctgtaa gaggtcgccg ctgccagcca ggaacaccca gcgcccagct
    32641 cgttgatccc ttcctgcaga atctgacctt tctcgtcttc tttatagtaa gcaacctgct
    32701 cgcggtcctg cggggtgtac tgctgaccgt tcgggctgta aataccaatc tgacggaaca
    32761 gaccttccat accgaaagta cgcgcttcgt cggcgatgat cggtaccaga cgatctttga
    32821 tcgacttgtt cttcagcatt acgttcaggg cacgaacgaa agcgatagtg gtagagatct
    32881 ctttgctctg ctcttccagc agcgcgccga agtcttgcag gctcggcagc tcaagcttct
    32941 cggtgaagtt cggctgacgg cttggcagat aaccgtgcag tttctgacgc tgagcgtgca
    33001 gataggtatg ctcttcagaa ccttccggga aggtgatgta cggcaatttt tcgatatcgg
    33061 catcagacac cggcacattg aaacggtcgc ggatgtgacg cacgccgtcc atgttcattt
    33121 tcttaacctg gtgcgcgatg tttttacctt cagccgcgtc gcccatgccg taacctttaa
    33181 tggtatgagc aaggattacc gtcgctttgc ctttggtttc ctgcgctttc ttaaatgcag
    33241 cgtagatttt cttcggatcg tgaccaccac ggttcagtgc ccagatctgt tcgtcagtcc
    33301 agtctgcaac cagtgctgcg gtttcaggat atttaccgaa gaagtgttca cgaacgtacg
    33361 caccatcttt cgatttgaag gtctggtagt cgccgtcaac ggtttcgttc atcagctgga
    33421 tcagtttacc gctggtatct ttacgcagca gttcatccca acggctaccc cacatcactt
    33481 tgatcacgtt ccagccagca ccttcgaaga tgccttccag ttcgttgatg atcttgccgt
    33541 taccggtgac cgggccgtca agacgctgca ggttacagtt gataacgaaa accaggttat
    33601 ccagtttttc acgggtagcg atggtgatcg cacctttgga ttccggttcg tccatttcac
    33661 cgtcgccgag gaacgcgtaa acggtttgtt tagaggtatc tttcaggcca cggtgttcca
    33721 gatatttcag gaatttagcc tggtaaatag caccaatcgg ccccagaccc atagatacgg
    33781 tcgggaactg ccagaattcc ggcatcagtt tcgggtgcgg ataggaagag aggccattgc
    33841 cgtgaacttc ctgacggaag ttatccagct gctcctgagt cagacgacct tccaggaaag
    33901 cacgagcgta aacgcccggg gagatgtggc cctggaagta aaccaggtcg ccgccatcct
    33961 gctcgttgcg tgcacggaag aagtggttaa agcacacatc ataaatggtt gcggaagact
    34021 ggaaggacgc catgtggccg cccagttcga ggtctttttt cgacgcacgc agaaccgtca
    34081 tgatggcgtt ccagcggata gctgaacgaa tacggcgttc cagttccaga ttacccggat
    34141 actccggttg ttcttcaacg gggatggtgt tgatgtagtt gctgataccg gtgcctgcgg
    34201 ctacgttgac accgcctttg cgggcttcag caagcagttg gtcgatcaga tactgagcac
    34261 gctcaacacc ttcttcacgg atgaccgatt cgatcgcctg gagccagtcg cgagtttcga
    34321 tcggatccac gtcatttggg aaacgttctg acatgggtta ttccttatct atctaataac
    34381 gttgagtttt ctggaacctg tcccattgaa ctctcgccgg aaagctcaat aagacaggtt
    34441 ctacgtttag ttgccgcgct tttatatgcg cttgatttac aacatcttct ggataatttt
    34501 taccagaaaa atcactaatt ctttcgttgc tccagacgac gtagagaacg ctcacggcgg
    34561 ctctcttcac ggcttctgtc gagcaaaatt tcttcgataa aggccagatg gcgatgcgat
    34621 gcttcgcgcg cttcttccgg cttaccggcc ataatcgctt caaatatgcg ggtgcggtga
    34681 ctactcacca gcggcagcat ctcgcgacgc gaatagagca attcgaagtt ctggcggaca
    34741 ttctgggcca acatcggctc catacacctt agcagatgaa gcagaaccac attgtgggcc
    34801 gcttcggtga cggcaatctg atactggagt acggcatttg attccgcgtc cagatcgcca
    34861 gactgctgcg ccagctctat ggcatggtgg agttcacgga tgcgttcctt gtcttcatcg
    34921 gtactacgca gcgcggcgta ataagcggcg ataccttcca gggcgtgtcg tgtttcgagc
    34981 aagtcatact gtgattcagg atggtcggag agcagctcca ccagcggatc gctgaagctt
    35041 tgccataggc tgctctggac aaaagtgccg ccaccctggc gacgaagcaa caagcccttc
    35101 gcttcgagac gttgaatcgc ctcacgcaag gagggacggg agacgtcgaa ctgttttgcc
    35161 agttcgcgtt ccggtgggag tttttcgccc gggcggagag tgccttcgag gatcaaaaac
    35221 tccagttgct gctcaatcac atcggagagt tttggttggc ggattttgct gtaggccatg
    35281 agttcctgtc ttaagccact tgccgaagtc aattggtctt accaatttca tgtctgtgac
    35341 gctaaagtaa caaagtattc accttatgtc catacaggtt ttgattgaaa tcatgaaact
    35401 gtgcacattt taacaacttg acatatataa cgtttcaaag ttgtaactat gcacaaatgt
    35461 taaatttacc catcaagggg tgagattaac agaattgaaa cgaaaaaata tgtcctctga
    35521 accgattcac caagcaacaa aacgcataac aattcaatta tg
//
LOCUS       DAZGHF010000037        35493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGHF010000037 DAZGHF010000000
VERSION     DAZGHF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.037"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..437)
                     /locus_tag="ACHK3A_001913"
     CDS             complement(<1..437)
                     /locus_tag="ACHK3A_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO8739994.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGV"
     gene            complement(640..1041)
                     /gene="nikR"
                     /locus_tag="ACHK3A_001914"
     CDS             complement(640..1041)
                     /gene="nikR"
                     /locus_tag="ACHK3A_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO8739995.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1046..1852)
                     /gene="nikE"
                     /locus_tag="ACHK3A_001915"
     CDS             complement(1046..1852)
                     /gene="nikE"
                     /locus_tag="ACHK3A_001915"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO8739996.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1849..2613)
                     /gene="nikD"
                     /locus_tag="ACHK3A_001916"
     CDS             complement(1849..2613)
                     /gene="nikD"
                     /locus_tag="ACHK3A_001916"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO8739997.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENTARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2613..3446)
                     /gene="nikC"
                     /locus_tag="ACHK3A_001917"
     CDS             complement(2613..3446)
                     /gene="nikC"
                     /locus_tag="ACHK3A_001917"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO8739998.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3443..4387)
                     /gene="nikB"
                     /locus_tag="ACHK3A_001918"
     CDS             complement(3443..4387)
                     /gene="nikB"
                     /locus_tag="ACHK3A_001918"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO8739999.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4387..5961)
                     /gene="nikA"
                     /locus_tag="ACHK3A_001919"
     CDS             complement(4387..5961)
                     /gene="nikA"
                     /locus_tag="ACHK3A_001919"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8740000.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6072..6659)
                     /gene="acpT"
                     /locus_tag="ACHK3A_001920"
     CDS             complement(6072..6659)
                     /gene="acpT"
                     /locus_tag="ACHK3A_001920"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO8740001.1"
                     /translation="MYRIVLGKVSTLSAAPLPPALREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLDNLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6714..7763)
                     /locus_tag="ACHK3A_001921"
     CDS             complement(6714..7763)
                     /locus_tag="ACHK3A_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO8740002.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7895..9112
                     /locus_tag="ACHK3A_001922"
     CDS             7895..9112
                     /locus_tag="ACHK3A_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8740003.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9116..9673)
                     /gene="dcrB"
                     /locus_tag="ACHK3A_001923"
     CDS             complement(9116..9673)
                     /gene="dcrB"
                     /locus_tag="ACHK3A_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO8740004.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(9746..10411)
                     /locus_tag="ACHK3A_001924"
     CDS             complement(9746..10411)
                     /locus_tag="ACHK3A_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO8740005.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10633..10878
                     /gene="tusA"
                     /locus_tag="ACHK3A_001925"
     CDS             10633..10878
                     /gene="tusA"
                     /locus_tag="ACHK3A_001925"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO8740006.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDELPYRYLIRKGG"
     gene            complement(10980..13178)
                     /gene="zntA"
                     /locus_tag="ACHK3A_001926"
     CDS             complement(10980..13178)
                     /gene="zntA"
                     /locus_tag="ACHK3A_001926"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO8740007.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVMLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13252..13878)
                     /locus_tag="ACHK3A_001927"
     CDS             complement(13252..13878)
                     /locus_tag="ACHK3A_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO8740008.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14019..14378
                     /locus_tag="ACHK3A_001928"
     CDS             14019..14378
                     /locus_tag="ACHK3A_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO8740009.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDI
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14381..14650)
                     /locus_tag="ACHK3A_001929"
     CDS             complement(14381..14650)
                     /locus_tag="ACHK3A_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO8740010.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14640..15236)
                     /gene="rsmD"
                     /locus_tag="ACHK3A_001930"
     CDS             complement(14640..15236)
                     /gene="rsmD"
                     /locus_tag="ACHK3A_001930"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO8740011.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15386..16882
                     /gene="ftsY"
                     /locus_tag="ACHK3A_001931"
     CDS             15386..16882
                     /gene="ftsY"
                     /locus_tag="ACHK3A_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO8740012.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPELVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16885..17553
                     /gene="ftsE"
                     /locus_tag="ACHK3A_001932"
     CDS             16885..17553
                     /gene="ftsE"
                     /locus_tag="ACHK3A_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO8740013.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17546..18604
                     /gene="ftsX"
                     /locus_tag="ACHK3A_001933"
     CDS             17546..18604
                     /gene="ftsX"
                     /locus_tag="ACHK3A_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO8740014.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18849..19703
                     /gene="rpoH"
                     /locus_tag="ACHK3A_001934"
     CDS             18849..19703
                     /gene="rpoH"
                     /locus_tag="ACHK3A_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO8740015.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19975..21078
                     /gene="livJ"
                     /locus_tag="ACHK3A_001935"
     CDS             19975..21078
                     /gene="livJ"
                     /locus_tag="ACHK3A_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO8740016.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQVGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21266..21649)
                     /gene="panM"
                     /locus_tag="ACHK3A_001936"
     CDS             complement(21266..21649)
                     /gene="panM"
                     /locus_tag="ACHK3A_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO8740017.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            22073..23182
                     /gene="livK"
                     /locus_tag="ACHK3A_001937"
     CDS             22073..23182
                     /gene="livK"
                     /locus_tag="ACHK3A_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO8740018.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANKGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            23230..24156
                     /gene="livH"
                     /locus_tag="ACHK3A_001938"
     CDS             23230..24156
                     /gene="livH"
                     /locus_tag="ACHK3A_001938"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO8740019.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            24153..25430
                     /gene="livM"
                     /locus_tag="ACHK3A_001939"
     CDS             24153..25430
                     /gene="livM"
                     /locus_tag="ACHK3A_001939"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO8740020.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGSFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            25427..26194
                     /gene="livG"
                     /locus_tag="ACHK3A_001940"
     CDS             25427..26194
                     /gene="livG"
                     /locus_tag="ACHK3A_001940"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO8740021.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            26196..26909
                     /gene="livF"
                     /locus_tag="ACHK3A_001941"
     CDS             26196..26909
                     /gene="livF"
                     /locus_tag="ACHK3A_001941"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO8740022.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            26997..27218
                     /locus_tag="ACHK3A_001942"
     CDS             26997..27218
                     /locus_tag="ACHK3A_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO8740023.1"
                     /translation="MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEF
                     ERYQKAKLDAEFDAIMAIHGSEIKELADK"
     gene            27215..27592
                     /locus_tag="ACHK3A_001943"
     CDS             27215..27592
                     /locus_tag="ACHK3A_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO8740024.1"
                     /translation="MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNK
                     YEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALFITLLFLKRNGIILAANPEFVEL
                     TVDVAAGKVSLEDIVTRLRQRGT"
     gene            27801..29117
                     /gene="ugpB"
                     /locus_tag="ACHK3A_001944"
     CDS             27801..29117
                     /gene="ugpB"
                     /locus_tag="ACHK3A_001944"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO8740025.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            29293..30180
                     /gene="ugpA"
                     /locus_tag="ACHK3A_001945"
     CDS             29293..30180
                     /gene="ugpA"
                     /locus_tag="ACHK3A_001945"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO8740026.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            30177..31022
                     /gene="ugpE"
                     /locus_tag="ACHK3A_001946"
     CDS             30177..31022
                     /gene="ugpE"
                     /locus_tag="ACHK3A_001946"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO8740027.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            31024..32094
                     /gene="ugpC"
                     /locus_tag="ACHK3A_001947"
     CDS             31024..32094
                     /gene="ugpC"
                     /locus_tag="ACHK3A_001947"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO8740028.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLKGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVLRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            32091..32834
                     /gene="ugpQ"
                     /locus_tag="ACHK3A_001948"
     CDS             32091..32834
                     /gene="ugpQ"
                     /locus_tag="ACHK3A_001948"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO8740029.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32821..33261)
                     /locus_tag="ACHK3A_001949"
     CDS             complement(32821..33261)
                     /locus_tag="ACHK3A_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO8740030.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            33381..35126
                     /gene="ggt"
                     /locus_tag="ACHK3A_001950"
     CDS             33381..35126
                     /gene="ggt"
                     /locus_tag="ACHK3A_001950"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO8740031.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGEYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
     gene            complement(35164..35448)
                     /locus_tag="ACHK3A_001951"
     CDS             complement(35164..35448)
                     /locus_tag="ACHK3A_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO8740032.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFIQGWYFCFESQEFL
                     ESGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         8256 a         9170 c         9942 g         8125 t
ORIGIN      
        1 acgccgccgc gcaccagcca gagtgactcg ctgcggctgt aaccgtcctc accgggaaac
       61 aggtgctcaa aatacaggct tctgccgccg ttatcactga gtatcagtgt gttatcgcgc
      121 agctgtaagc ggatatccgc aggcattttc cagccggggc cgaggctccc caccggcgcg
      181 ggcgtttttg tccggtaact gctgtaggtg cgggagagaa tgaacggcag cgggccgggc
      241 agggcaaggt cggtttcgcc gggaaggacc ttcgcaccga gcagcggatt gaccggatga
      301 ccggacgtca ccccgccggg gcacaccgaa caggccacac cggtgggggc accaatacgt
      361 accccggcag aaccctgaac aatgctaccg ccatactgcg tcatgtcgcc ctgacgcgcc
      421 gccggttttc cgctcatcgt aaaatccttt tctattattt taatgacagt atcttcatac
      481 gaaaaagcga agaaaaactt gtggtcacaa tgaattgaag atttttagga atatatttag
      541 aacgttacaa aagagaaggt tcggataggg tatttagaga ggaatcaggt gtgctagtgg
      601 ccctttgttg atcgttcacc aaaggaccca ataataggct taatcttcct tcggcaagca
      661 ctgcaaatgc ccgtgccgca cgccgcgctg ggcgataacg tcatcggcaa aatgctgcac
      721 gtcgcccata tcgcctttca gcacggcgat ttccagacag tcgtcgtgat tgatgtggac
      781 atgtagcgtc gcgacggaga gatcgtggtg atggtgctgg gtggagacaa tacggctggc
      841 taagtcgcgt ttttcgtgtt catacacata ggagagcacc gcgaaacctt gcgtgccgtg
      901 ctgctgggta gcttcctgcg ccagggcgct acgcagaata tcgcggatag cttcggaacg
      961 gttgttatag ccacgacgct ggctcaggct gtccagcgtc tccagtaaat cgtcatcaag
     1021 cgtgatggta actcgttgca tagacttaaa ccttttctgt ggtgcgacgg cgcacgggga
     1081 atgcgggtaa taccgcgttt tgtagcacac gtccggcgtc agaggaaaag gttaatttat
     1141 ctcccaccac ctgggtttcg acgatttgtc cgttgtccat caccatcacc cgctgacaga
     1201 agcgttccac caggcgtaag tcgtgggtga tgaacaggca ggcggtgcca aactgttgtt
     1261 gcagcttttt cagcaggcga atgacgcccg cctgtaacac gagatcaagg ttagaaacgg
     1321 cttcatccag aatcagtagt ttcggttcga ccgccagcgc gcgagccagg cagacgcgct
     1381 ggagctgacc gccgcttaac tgcggtgggc gtttgtctag aacgctgtca tcgagatcga
     1441 ccgccttcag catttcgctg gcacgcgcca gttgttcggc ttttttcagc gatagcaggt
     1501 ggcgcatcgg ttcacgcagg atctcgcgca cggtttttcg cggattcacg gcgctgatgg
     1561 agtcctgaaa taccatctga atatcgcggc ggaacgcttt acgctgggcg cgattgagct
     1621 ttgccaacgg ttcgccgcgc cagctaatat tcccttgtga gggagactct aagcctacca
     1681 gcagccgcgc gagagtgctt tttccgcagc cgctgcgccc cagcagagcg acggtttcgc
     1741 cgcttttcag ggtcagggaa acgttattca gtaccgcctg atgctgatgt tttccgctaa
     1801 atccaccgtg cgcatagtga tgtgaaaggt cgcagacgtt aagtaaagtc atgatgccag
     1861 ctccataccg tagagggcga gatgagcgga aaccaggctg cgcgtcaccg tatgtttggg
     1921 ggcgttaaac agcgtttcta catcgccctg ttcgacaatt ttaccgtgtg acatcaccgc
     1981 cacgtcatcc gccagacgcg ccacaacgcc catatcatgg gtgaccagca gcattcccgg
     2041 tgcttgtttt tgcataatgc tttccagcag atcgaggatg cgcgcctgtg ctaccacgtc
     2101 gaggtcggtg gtcggttcat cggcgatgat aaacggcgat tcacacaaca ccgccatcgc
     2161 aatcatcatg cgctgcaaca tgccgccgct catctcgaac gggtacagct tcaacacgcg
     2221 cgcggtgttt tccaacccca ccgcttctat ggcagcggta agcgtagcgt catcggcggg
     2281 tttccccagc gccaggcagg tttcacgcgc gtgggtgtgc atggtgtgta gcggattaaa
     2341 ggcgctgcgc gggttctgca tgatggtggc aattttgatg ccgcgcaggg cacagggaga
     2401 aaccggtttg ccatcggcta aaatttcccc cgccgtctgg cgaacgcctg cgggcagaat
     2461 gcccagcgtc gcggcgcagg tcagtgattt cccgctgccg ctaccgccga ctaacgccag
     2521 cacacgcccg cgtttaaggg ttaacgatac gccgtgcacc agcggttgtg cggcctgtag
     2581 cgcgatatta cgtagttcaa tctgttgcgg cattagtgtg cgtgctccgt caccagatga
     2641 ggatccagat gatcgcgcag tgcgtcaccc accaggttaa aggccatcac gctgataaac
     2701 agcgccagcc ccggccagaa catttgcagc ggctgggtcc agatatactg acgcgcgtcg
     2761 ttaatcatca cgccccattc ggcggtcggc gcggtcacgc cgagaccgag gaaggacatc
     2821 cccgcgacgt gtagcatcat atgaccgata tccagcgtcg ccagcaccag cagcgagggg
     2881 atcaccgcac ctgccagatg atcaataaac acccgcacat ggcccgcgcc agaaagccgt
     2941 gatgccagca caaactcgcg ctggcgcagt gaaatcacca ggctacgcac catgcgtgca
     3001 taccacgccc agtgcgacag ggcgatggct ataatcacat tggtcaaccc ggtgccgagc
     3061 acgccgacca taaagaacga cagaatcgag gttgggaagg tcataaacat atcagcgacg
     3121 cgcatggtgg cctgatcaac gcgcccgcca atcagcccgg cgctgccgcc aataatcagc
     3181 cctaatgtca gcaccaacag caggcaggcc attaccgagc cgagcgacac gcgggtcgcc
     3241 gccatcagcc gcgagaaaat atcgcgccct aaatgatcgg tacccagcca gtgctgcgca
     3301 tccggtgaaa gcaggcgcga cggcagatca atcgcctggg ggtcatacgg cagccaccac
     3361 tggctggtga gcgcaatcag cgccagcagg gcgataatga tcagcgccag gcgtaccgac
     3421 cagcgggaag agaggaaaaa gttcacgcgt gcgctccttc atgacgacga atgcgcgggt
     3481 ccagcgcggc gttgagtaaa tcaacaatca aattacagac cacaaaaacc accaccatca
     3541 tcagcgtaaa gcactggatc accggatagt cacggttaaa aatcgccgac accgcatagc
     3601 gcccgacgcc cggccaggca aagatgtttt cgataatcat cgttccgcca atcagttcgc
     3661 caatgtgcat ccccacggcg gtgatcatcg gcagcgaggc attgcgcaga atatggcggc
     3721 gttcggtctg tttgtcgttc aggccgcgta gccgagccca ggtgacgtga cgctgaccgg
     3781 cgacgtccag catactggcg cgcagcagcc gcgcgttaat cgccagcgac ataaaggcaa
     3841 tggaaaccgc aggcaaaatg atgtgctgcc agccgccgta gcccatcgcg ggtagccatt
     3901 gcagatatac cgaaaacgcc atcaccagca aaaacgccag ccagaagttg ggcatcgaca
     3961 cgccgagaaa cgcgatgaaa cgtacggcga aatccggcag acgatcgcga tggcgcgccg
     4021 cccagatacc gagcggtacg gaagtgagca gaattaacac cagcgcggca cctgcaagtt
     4081 ccagcgtggc gggcaggaag ttcagcatat cgtccagcac cgggcgttgg ctggcgaatg
     4141 agataccaaa gtcaagatgc agcgccttcc acaaccaggt accgtactgg acgtacagcg
     4201 gctgatccag ccccagcatg gtgcgggtag aggccagcat ctccggcgtc ggcggcaggt
     4261 tagataaacg cagataatcg agcgccgggt cgccggtacc gaggcgcagc atcagaaaaa
     4321 tgatcaccga ggcggcgagc accatcggga tcagcagcag aaagcggcgt aatacgtaac
     4381 gcaacattaa ggtttcaccg gtttaatctg ttcgaacgga atttcggtgg cgatcggcgc
     4441 gtaggggatg ttacccagct ccggttttga taccaccatc attgagatgt aactgatagg
     4501 cagataaacc gcctcgtcat gcagacgggt cagaatgtcg cgatacagcg cctgacgttg
     4561 cgtttcgtca tgggtcgcca gcacttcgcc gatctcttta tcaatcagcg gcttgtcggc
     4621 taaaccttgc tgtgcctgga agtcagcgtg cgacggtacg cgcattgaac tcaggaaggc
     4681 gtgtggatcg tatggcgcgc cccaggtgcg gtggaaaatc atgccaaaac gaccgtcgcg
     4741 ctgacgagca tagatactgc tctcttcttc gccaatcagc gagacatctg cgccaatctg
     4801 gcgcatatca gcctgaatga tttccgccat cgatttgctt aacgcatcgg tgccgatgaa
     4861 cgacagttca atgcgcagcg gctgaccatt tttctcgcgg atgtctttgc ctgcaggcag
     4921 cgtccaaccg gctttttcca gcaacgcttt cgctttctgc ggatcgtact ggcgcggttt
     4981 caggccgagg ttggcgtagg gcacagaagg ggcaaacagg gtgtcggcga cctgctgggt
     5041 accatacaac gcgttatcaa tcagcgattt tttgttcacc gcgtaattaa gagcttcacg
     5101 tactgccagc tcgttggtgg gggctttggc ggtattgagc gccagcatca cggtttcgat
     5161 cggctgtgac agttgagtgt ggtaagccgg gttctggcta aagcgggcga aggtatcgag
     5221 cggtaataac ccttcgtttc cgtacagcag gtcgatatcg ccagtttcaa acgccaccgc
     5281 acgggtagtc gggtccggga tcacgttaaa ggtgatcttt ttaatcgctg gcttttcgcc
     5341 ccagtagttt tcgttacgga cgaagacatc gtactgattc agtttcgatt cctgtaaaac
     5401 ccacggcccg gtgccaatcg gcgctttaat tccgttcatg gtttcgtggt ttttaaactg
     5461 cgagggtgcg ataaaacgga aaggacgggg cagggccagt tcttgcagga aaggatagta
     5521 ggcgcttttc agggtgattt gcagctcgtt tttactgagt gctttgacat caacaatctg
     5581 gtttgccagc tccagccagg cgtgacgttg acggttatcg agcactgcgc ggaagttttc
     5641 tgccgccgcc tctgcatcaa acggttcgcc gttggagaat ttcacgtcat cacgcagggt
     5701 gaaggtccag gttttaccat cttctgaatg agtccagctt tttgccagcc acgggatcac
     5761 cgaaccgtct gcctgatatt tcaccaatgg ttcataaacc atgctctggg cgaacatctg
     5821 gttaggcgtg taaaggtgcg ggtttagtgg cccgacattc accggccagg cggtggtgat
     5881 ttcatctggt gcagcggcat ggacgataaa agacgcacaa gccagcagcg caaatagagt
     5941 gcggcggagt gtggagagca tggtaacccc aatggattaa aatagatggc ggaaataagt
     6001 atgacgattt taagtattcg tcatactgat tacctgttct ggatcaatag atgggcagtc
     6061 gggtgggcgg atcagttaac tgaatcgatc cattgcacac tgtcggcggt gagcgtaaag
     6121 ggggtagggg tgcagatcgc caggcttaaa ttgtctagct gacaatggct gactgacagt
     6181 gatgagtgat aggtgctgtc tacgctgacg atttgccagg cgctgccgcc acgctgttta
     6241 acgatggctt ctttgcgcgt ccagatgcgc cagaacattt ccagttgctg atcaggatgc
     6301 acggcgtcca tctcggcatg ttccccaagg ctgaatacgg cgttcgccag ccagcgccag
     6361 ttggcgcgcg ggcgaatcac ttcgatatcg cagccaactt cgccttcatc gctcaacagc
     6421 agggcgatat cgtcaccgct atggcttaag ttgaaccata gcggcgtttc cggcgcgaat
     6481 gccggtttgc cttgttcgcc atagatgatc tccggtagcg gggaaagcgt gtgcgaaagc
     6541 aatgcacgcc ccgccagcca gcgttcgcgt cgtggacctt gcggtgcttg ctcgcgtaaa
     6601 gccggtggca gtggagctgc gcttaaggtc gaaactttcc ccagaactat ccgatacata
     6661 tcagggccaa cgtttaatgg aaaatgaaag tgcgtatcgt aacacttgtc gcctcatccc
     6721 ggtaaccgac ttttcggtct gcctggcccc agtaaaatcg ccagtttgct accgcctttg
     6781 gtggtttcca tccagatttt acacacgctg gttaatggca ccgaaagtag catccctacc
     6841 gggccgagca gccatcccca aattaacaat gaaagaaata ccaccatggt ggacatcccc
     6901 agacgatggc ccatcatccg tggttctaaa atattgccga tgaccatatg gaccactaaa
     6961 aacaatgcgc cgaccagaat acattcgtaa ataccattaa acagcagcac ctgaatcatt
     7021 ggcggtacgg cggaaattac cgcaccgata ttgggcacgt agttgagcaa aaacgccagt
     7081 accgcccaca tcagcgcaaa ctgtacgccc ataagttcca gccccagcca gacgataacg
     7141 cctgtccata aacttagcag tgttttcaat gccagataat gcgaaacgcc tttaagtgcg
     7201 cggtgtagtc cggcgatgtg aatttgtgga ttattcaacg cgaaacgcat tttgtaaggg
     7261 acgtggcgca cttcaaacag cataaaaact acggtcatca ccagcaaaag cacgctcgcc
     7321 attgccccgg aaagcccggt cattagcgct gtggtgaagg taaccacttt ttccgagtcc
     7381 atccgctgca gcattcgctc cggtgacata tgcaaattaa gaaaaggcaa catctcctgc
     7441 aatttaaaaa gtttgcgcgt cagctcctta ttaaacttcg gcagcataga gataaattcg
     7501 ttaaacgatg ccgccagtac gccgaccagc gcagttagtg cgatcagcat caccaccacc
     7561 acaatcgtaa tggcaacggg gcgttgtact ccccgacgaa taaaccaggt gacgagcggg
     7621 ttgaggacga tggcaaaaaa cagcgccagt aacagctgca caatgatatc tgccgctgcg
     7681 tgaatgcccg cgaggatcac taccagcgag gccagcttga gcagaatgtg catgcccgtt
     7741 ttatcgggtt gaggggtttc cattggggct tccttgtgac tttttgtatt aagtgtagcg
     7801 ggagtcacgc cagcaatatt ctgattctta ctgctgattt tcacgtcagc acgtggtaaa
     7861 aatgaaacac tgttgtaaaa atgtggtgat cctcatgccc gaacccgtag ccgaacccgc
     7921 gctaaacgga ttgcgcctga atttgcgcat tgtctccatt gtcatgttta acttcgccag
     7981 ctacctcacc atcgggttgc cgctcgctgt attaccgggc tatgtccatg atgtgatggg
     8041 ctttagcgct ttttgggcag gattggttat cagcctgcaa tatttcgcca ccttgctgag
     8101 ccgccctcat gccggacgtt acgccgattt gctgggaccc aaaaagattg tcgtcttcgg
     8161 tttatgcggc tgctttttga gcggtctggg atatctgacg gcaggattaa ccgccagtct
     8221 gcccgtcatc agcctgttat tactgtgcct ggggcgcgtc atccttggga ttgggcaaag
     8281 ttttgccgga acgggatcga ccctgtgggg tgttggcgtg gttggctcgc tgcatatcgg
     8341 gcgggtgatt tcgtggaacg gcattgtcac ttacggggcg atggcgatgg gtgcgccgtt
     8401 aggtgtcgtg ttttatcact ggggcggctt gcaggcgtta gcgttaatca ttatgggcgt
     8461 ggcgctggtg gccattttgt tggcgatccc gcgtccgacg gtaaaagcca gtaaaggcaa
     8521 accgctgccg tttcgcgcgg tgcttgggcg cgtctggctg tacggtatgg cgctggcact
     8581 ggcttccgcc ggatttggcg ttatcgccac ctttatcacg ctgttttatg acgctaaagg
     8641 ttgggacggt gcggctttcg cgctgacgct gtttagctgt gcgtttgtcg gtacgcgttt
     8701 gttattccct aacggcatta accgtatcgg cggcttaaac gtagcgatga tttgctttag
     8761 cgttgagata atcggcctgc tactggttgg cgtggcgact atgccgtgga tggcgaaaat
     8821 cggcgtctta ctggcggggg cagggttttc gctggtgttc ccggcattgg gcgtagtggc
     8881 ggtaaaagcg gttccacagc aaaatcaggg ggcggcgctg gcaacttaca ccgtatttat
     8941 ggatttatcg cttggcgtga ccggaccact ggctgggctg gtgatgagtt gggcgggcgt
     9001 cccggtgatt tatctggcgg cggcgggact ggtcgcaatc gcgttattac tgacgtggcg
     9061 attaaaaaaa cggcctccgg aacacgtccc tgaggccgcc tcatcatctt aaaacttact
     9121 gaataaccag cgtattaatg atgttttctg cggtggtctg cgctttttgc tgattgtcag
     9181 ctggcagcgt aatttgcatg gtcagcagtt gattacccac gttacccaga ataacggaag
     9241 agtacgccgt ctggcctttc gcggagataa tgctgtctaa ctgctgcatt ttgtgacctt
     9301 tcagctcaat ggctttattg gttaccactt gcagctgcgg atcgcggcta cgttgctgat
     9361 cttccagacg cttcgccaac accgccagat cttctttcgg atcatcgccc atgatgacga
     9421 tgactgcttt ttgcccagtg gcgtcggacc agacatgcat gttattggcc tgcgttccca
     9481 gcttaccgct ctggtcggtc atatccgctg gcagcgagaa acttaacttg ccatcaagca
     9541 ggttgacggg attcccggta gcgttacttt ccgcgaccga cccctgcgcc gtagcgttag
     9601 tgtctttatc atcacaggcc gcaagcccca taaccagcag gccaattccg acatatttaa
     9661 ccagattgcg cattgacttc ttcctttcga taaacggcca taacggctca ttcatccatc
     9721 ttatcacaac tctgataacg aacctttaac tcgcctgcaa agcgttgatt tcagatttat
     9781 ctgccagtct tttcaacagc atattgagta atacgccata cattggcagg aagaaaacga
     9841 tactgattaa cactttgaaa cagtaatcga ccagcgcgat ttccatccag tgttcagcca
     9901 taaaggcatc cgggctacgc cagaaggcaa tgaagaagaa ggccagcgtg tcgctgacgt
     9961 taccgaacag tgtggacgct gtcggtgcca gccaccagtg acgactctga cgcaggcggt
    10021 taaaaacgtg cacgtcgagg atctgcccca gcgcgtaggc catgaaactg gcggtggcga
    10081 tacgggcgac aaacaggttg aagtgggcga gcgcgccgaa tccctgccag gaacccatat
    10141 agaacagcga cgagatgacg taggagatca gtaacgccgg gatcatcacc gcaaagatga
    10201 tacgacgggc cagcggtgcg ccaaaaatac gcacggtcag gtcggtagca aggaaaataa
    10261 acggaaagct aaatgcgccc caggtggtat ggaaaccaaa aatggagacg ggaagctgaa
    10321 ccagatagtt actggaggtg atcaccagca gatgaaataa cgataaccag aacaacgcct
    10381 tatagcgttg agtttgcgag aaaacgttca tattgtacct ttttgattaa ccattggggt
    10441 gagggaaccc aatacgtacg acacgctatt ttaccttgat taaataacgt tcttaccctt
    10501 ttttcgagcc gccgcatgat actgctttac gttgacaatg caatggttaa ttttcacgca
    10561 atcgttaacc tggtttgctt acggactcac agggcgtaaa ctagcgccgt ttttttatgt
    10621 gatgagaaga aaatgaccga tctcttttcc agccctgacc acacactcga cgcacttggc
    10681 ctgcgctgcc cggaaccagt aatgatggtg cgcaaaacag tgcgcaatat gcagccaggc
    10741 gaaacgttgc tgattatcgc cgacgatccg gccactaccc gcgatattcc tgggttttgt
    10801 acctttatgg aacacgaact ggttgctaaa gagacggatg aactgcctta tcgttatttg
    10861 attcgcaaag gcggttgatg gggaaagatt ggcttcgatg ccgccttttc ccctcaccct
    10921 aaccctctcc ccagaggagc gaggggaccg atcgcgctca atgttgcgat cggtttgcct
    10981 tatctcctgc gcaacaatct taacgcattc gctgtcacca gcaccgtcgc acccgtatct
    11041 gccagcaccg ccagccatag cccggtcatt cctaacagtg tggtgacgag gaagatccct
    11101 ttcagcccca gcgcaatagt gatgttctgg cggatattgg cgtgagtggc gcgtgccagt
    11161 tcaatcattt gcaccaggcc gcgcaggtgg ttatgggtta atgctgcatc ggcggtttcc
    11221 agcgccacgt ctgtgccact gcccattgca atcccgatgg cggcggcttt catcgctggc
    11281 gcgtcgttaa taccgtcacc gaccatcgcc agcggcgcat gttgattcag ctcggtcacc
    11341 gctttgactt tatcttccgg caacaggccc gctttaaact ctagccccag ttccccggcg
    11401 attgccgccg ctgcgcgtgg attatcgccg gtgaggatca cccctttgac gcccagcgcg
    11461 ttcagttcac tgatggcagt tgcagcatcg gcgcgcaggg tatcctgtaa cgcaatgaca
    11521 ccaagcacgt catcgttacg tactaccagc actaccgttt gcccggcgct ttccagttcg
    11581 ttaattaaac cagcaaatgc atcagcggga tgtttcccgg cagcgcatat caacacgcgc
    11641 tcaccgttaa cctgcgcttc aatgccagac ccaaccagcg cccgctgtga ttgggcggcg
    11701 ggaatggcga gttcagcaac ctgtgcttct cgcacgatgg cttgcgccag tggatgcgtc
    11761 gcgccttgct cgaccgccgc tgccagtgtc agcagttcgg attcactaat acccgttgcc
    11821 ggatgaatcg cggtaacgcg cggtttaccg acggtcagcg taccggtttt atcaaacgcc
    11881 acctgggtaa cacgacccag ttgttccagc gccgctccgc ctttaattaa cgccccacga
    11941 cgcgctgctg ctgccagccc ggaggtaatc gccgcaggcg ttgagataac taacgcacac
    12001 gggcagccaa tcagcagcag cgtcagccct ttataaatcc actcttgcca gctggcggca
    12061 aacagcagtg gtggcaccag cgttaccagc agagcgacgg ccataatcgc tggcgtgtaa
    12121 atacggctga aacggtcgat aaaccgctca atgggagcgc gacgctcttc ggcttcttcg
    12181 atcagtttca gaatccggtc aatggcgctg gcacccggtt ctgacagcac ttccagcatt
    12241 accagacggt ctacgctggt ggcgcctgca ggaactttat cgcccgtcgc gcgctccaca
    12301 ggaatggatt ctccggtcag ggcgctttca tcaaaactgg caaacggtga gagcagttta
    12361 ccgtcggcag gcaaacgccc acctgccgca acttcaatca catcgccagg ccgcaggctg
    12421 tttattgcca cctcttcacg ctcaccgtta cgcagacgcg tggcggtttc tggcttcagc
    12481 gccattaacg cgctgacccc ttgacgcgcg cgactggcgg cccagccctc cagtcgttcg
    12541 ccaatcaaaa acagcagcaa caccatcgca gcttcagccg ttgcgccaat aaacagcgca
    12601 ccaatagcgg ctacgctcat taacgtttca atggcgaagt agctgccgga tttgatcagc
    12661 cgtaatgcct gacgagcaat cgggtacagc ccaaccagcg tggtcgcgat aaacgccagt
    12721 tgcccgaacg gatgattgaa ctgctccaga ccccagctga ttgccatcat cacgattagc
    12781 gtaatcagcg gcagattctc tttcaggcgt gatgcttgtg gttcgtcggc ggactgttca
    12841 tcgcgcaggg aatagcctgc tttttgcacc gcagattcaa cctgggcacg gatgtcattg
    12901 tcggcatcga ccaccagttt ttcggtggcg aacaacacct gcacctgatt cacgcctgca
    12961 agctggcgca cggcattttc taccttgcgc gcacaggcgg cgcagtccat gccgctgact
    13021 ttccagctat agcgggtgcc ggagacgttt tcagagagag ttggcgtgct ggaacatgcg
    13081 ccgtcgcagc aacagtcgtt ggcgttctgt accgtggtta gcggtttgaa cgcagcaaat
    13141 tgaggggctt tcttgccgtg attgtcagga gtcgacatgg catcctccgg ttaagttttt
    13201 tctcattaac cgaaggatac actctggagt cgactccaga gtcaagtttt atcagagata
    13261 cagcgagcgg acgatgagga aatgaccggc aaagtagcag gccgcagcaa tcgcgttatc
    13321 tgcccggaag cggcggcgat agtggctacc cagccagaca aagttactga taaacagcaa
    13381 cgaagcgccg acaaacgcag agagcgccgg agctgtcgga cggaagaacc acagttcacc
    13441 tgccagccac accatcacca gcgtcatgcc gataaacgtg cagataggcc aacgatactc
    13501 ttccagacgc gtccagataa tcgccagtaa cagcgcaccc agaaccagca acaccagtgg
    13561 cagaggccag aagaaagaga gcgtcatctg actggcaaaa tagatggtgt acagcaggtg
    13621 cgagaggaaa aacgcgccga tggcgtacat cagacgttga cgtggcaaca gggttagcgc
    13681 atcgcccagc agtgaggcgc acagccctgc cagcaccaga tagctaatgg cgtcgaacat
    13741 cggcgcttgc caggccagta acagcaggag aagaagggtt aacggtttaa acacccagcg
    13801 ttgccaggtt ggcccacgat acgatgcatc cacagatagc catgcggaaa gacagacagc
    13861 gataaacgac caaagcatct tagttccttg aattcgttat tttttcgtaa gcggaatgcc
    13921 tacggtcttg cttccagttt agtggcgagg gtggacggat gacaacacca taactggcag
    13981 ggtatgctta tttccgcatt ttccgatgag ggataaagat gagcaaacca cctcttttct
    14041 ttattgttat cattggctta attgtcgtcg ccgcatcgtt tcgttttatg caacagcgac
    14101 gggaaaaagc tgataatgat atggctccgc tccagcaaaa gctggtggtg gtgagcaaca
    14161 agcgggaaaa accgattaac gatcgccgtt cgcgccagca ggaagtgact ccggcaggca
    14221 ccagtatgcg ctacgaggca agcttcaaac cgcaaagcgg aggaatggag cagacgtttc
    14281 gcctcgacgc tcagcagtac catgccctga cagtgggcga tataggtacg ctgagctata
    14341 aaggaacgcg ctttgttagc tttgtaggcg aacaataagg ttatttttta actttaaatt
    14401 tcttctgcca gaccagcagt tcaaacacgc cgaaaaggaa aatccgcacc ttttcggcgg
    14461 tggtcatctg cgggccatct ttaggtaaag tggatttcaa tagcgccagc tgcataccat
    14521 gcatcagcac ggtaaaaatc agcgccacgt taacgaaaat attcagcggg cgcgggaagg
    14581 gatgcaccag attgaggatt aaaaatcccc aaacgcagag cattaacaaa cgaccaatat
    14641 taatcagcat cactttctcc ttgtgcttcg cgttgataca gccgataagc cacctgaccc
    14701 gccacttttt cccgatgtaa tgaccagttt gccggaacag tgggcagacc gttttcgact
    14761 tcgctttcga cataaatcag ggcttcgtca gccagccagc cgttatcttc cagtaaattt
    14821 atcgtctctt ctaacaagcc acggcggaac ggtggatcga caaacacgat attatgcggt
    14881 gtgccttttt gcgccaggaa tgacattgcg ttgctgttca ccacgcgtgc attgcctgct
    14941 tttagtgtcg ccagattctt aattaactgc tgagaaaccg cgcgatccat ctcaatcaac
    15001 gtggcccccg cagcatagcg cgacaacgct tccagcccca gcgcgccgct cccggcgaag
    15061 caatccagac attgggcgtc aacaatgacc ggagccagcc agttaaacaa cgtttcgcgt
    15121 acgcggtcgg tggtggggcg cagacctggg ctatcaggaa ccgggagttt acggcctcgc
    15181 cactgcccgc caataatgcg gatttggccg ctgccggaat gattcggttt tttcatgata
    15241 aattgctcaa tccgccagat aacacatact tgcaggcggt gatgtgaatt aagttaagtg
    15301 atagactatt tcatcatttt tttagctgct atgtacatag cgttaacgct gtgccatgaa
    15361 gcaacagcga ggagtgtagt cgcaaatggc gaaagaaaaa aaacgtggct ttttttcctg
    15421 gctgggcttt ggtcaaaaag agcagacccc ggaaaaagag acagaagttc agaatgaaca
    15481 accggttgta gaagaaatcg ttcaggcgca agagcctgcg aaggcttcgg aacaagccgt
    15541 tgaagagcag ccgcaggcgc atactgaggc cgaagcggaa acttttgctg ccgacgttgt
    15601 ggaagtcact gaacaggttg ttgaaagtga aaaagcgcaa cctgaagcgg aagttgttgc
    15661 acagccggaa ctggtcgtag aagaaacgcc ggagccagtg gctatcgagc gtgaagagct
    15721 gccgttgccg gaagacgtca acgccgaaga ggtttcgcca gaagagtggc aggccgaagc
    15781 ggaaaccgta gagattgtcg aagcggcgga agaagaagcg gctaaagaag aaatcaccga
    15841 cgaagagctg gaagcacagg cgctggctgc cgaagcagca gaagaggcgg tgatggtggt
    15901 tcctccggca gaagaagagc agccggtaga agaaatcgct caggagcagg aaaaaccgac
    15961 caaagaaggt ttcttcgcgc gcctgaaacg cagcctgtta aaaactaaag aaaatctcgg
    16021 ttccggattt atcagcctgt tccgcggtaa aaaaatcgac gatgatctgt ttgaagagct
    16081 ggaagaacag ctgttgatcg ccgatgtggg cgtggaaacc acacgtaaaa taatcaccaa
    16141 tctgacggaa ggcgcatccc gcaagcagct tcgtgatgcc gaggcgcttt atggcctgct
    16201 gaaagaagag atgggcgaga ttctggcgaa agtcgatgag ccgctgaatg ttgaaggtaa
    16261 aacgccgttc gtgatcctga tggtgggcgt caacggtgtg ggtaaaacca cgacgattgg
    16321 taagctggca cgtcagtttg agcagcaggg taaatcggtg atgctggcgg cgggcgatac
    16381 tttccgtgcg gcagcggttg aacagcttca ggtctggggt cagcgcaaca atattccggt
    16441 gattgcccag cataccgggg cggattccgc ctctgttatc ttcgacgcca ttcaggcagc
    16501 taaagcgcgt aatatcgacg tcctgattgc cgataccgcc ggacgcctgc agaacaaatc
    16561 gcacctgatg gaagagttga agaaaatcgt ccgcgtgatg aagaaactcg acgttgaagc
    16621 gccgcatgaa gttatgctga ctattgatgc cagcaccggg cagaacgcgg taagccaggc
    16681 caaactgttc catgaagccg ttggcttaac cggcatcacg ctaacgaaac tggacggcac
    16741 ggcgaaaggc ggagtaattt tctcggtggc tgaccagttt ggtatcccta tccgctacat
    16801 tggtgtcggc gaacgtattg aggatttgcg tccgtttaag gcggacgact ttatagaggc
    16861 actttttgcc cgagaggatt aacaatgatt cgctttgaac atgtcagcaa ggcttatctc
    16921 ggtgggagac aggcgctgca gggcgttacg ttccatatgc agccgggtga gatggcgttt
    16981 ctgaccggtc attccggcgc agggaaaagt accctcctga agctgatctg tgggattgag
    17041 cggcccagcg ccgggaaaat ctggtttagc ggccatgaca tcacgcgtct gaaaaaccgt
    17101 gaagttccgt ttctgcgccg ccagattggt atgattttcc aggatcacca tctactgatg
    17161 gaccgtactg tctacgataa cgtggcgatc ccgctgatta tcgccggtgc cagcggtgac
    17221 gatattcgtc gccgggtgtc ggcggcactg gataaagtcg ggctactgga caaagcgaag
    17281 aactttccta ttcagctttc cggcggtgaa caacagcgtg ttggcattgc ccgcgcggtg
    17341 gtgaataagc ccgcggtact gctggcggac gaaccgactg gtaacctgga cgacgcgttg
    17401 tcggaaggca ttttacgtct gtttgaagag tttaaccgcg ttggggtaac cgtattgatg
    17461 gcaacgcacg acatcaacct gatttcgcgg cgttcctatc gcatgctcac cctgagcgat
    17521 ggtcacttgc atggaggcgt gggccatgaa taagcgcgat gcaatcaatc atattcggca
    17581 gtttggcggg cgtctggatc gcttccgtaa atcggtcggc ggctcaggcg acggcggtcg
    17641 taacgcaccg aaacgcgcga aatcctcgcc aaaaccggta aatcgcaaaa ccaacgtttt
    17701 caacgaacag gtgcgctatg ccttccacgg cgcattgcag gatctgaaaa gcaaaccgtt
    17761 cgccacgttt ttaacggtga tggttatcgc catctccctg acgctgccca gcgtctgtta
    17821 tatggtgtac aaaaacgtta accaggcggc gacgcaatat tatccgtcac cgcaaatcac
    17881 tgtttatctg caaaaaacgc tggacgatga cgccgctgcg ggtgtggtgg cacagttaca
    17941 ggccgagcaa ggcgtagaga aagtgaatta tctttctcgt gaagacgcac tgggcgagtt
    18001 ccgtaactgg tctggttttg gtggtgcgct ggatatgctg gaagaaaacc cgcttccggc
    18061 agtggcggtg gtgatcccga aactcgattt tcaggggacg gaatcactga atacgctgcg
    18121 tgatcgtatc acccagatca acggcattga cgaagtgcgg atggatgaca gctggtttgc
    18181 ccgtctggcg gcgttaaccg ggctggtcgg gcgcgtttcc gcgatgatcg gcgtgttgat
    18241 ggtggcagca gtgttcctcg tcatcggcaa cagtgtgcgt ctgagcatct ttgctcgccg
    18301 tgactccatt aacgtgcaga aactgattgg tgcgacggat ggtttcatcc tgcgcccgtt
    18361 cctgtacgga ggcgcattgc tgggattttc tggcgcattg ctgtcattaa ttttgtcaga
    18421 aattctggta ctgcgtttgt catcggcggt tgcggaagtg gcacaggttt tcggaacaaa
    18481 gtttgatatc aatggcttat cgttcgatga atgcctgctg ttgctgctgg tatgctcgat
    18541 gattggctgg gtggcagcgt ggcttgccac ggtacaacat ttacgccact ttacgccgga
    18601 ataataaaag cgtgttatac tctttccctg caatgggttc cgtagcaggg aaagagaccc
    18661 cgttgtctct tcccggtatt tcatctctat gtcacatttt gtgcgtaatt tattcacaag
    18721 cttgcattga acttgtggat aaaatcacgg tctgataaaa cagtgaatga taacctcgtt
    18781 gctcttaagc tctggcacag ttgttgctac cactgaagcg ccagaagata tcgattgaga
    18841 ggatttgaat gactgacaaa atgcaaagtt tagctttagc cccagttggc aacctggatt
    18901 cctacatccg ggcagctaac gcgtggccga tgttgtcggc tgacgaggag cgggcgctgg
    18961 ctgaaaagct gcattaccat ggcgatctgg aagcagctaa aacgctgatc ctgtctcacc
    19021 tgcggtttgt tgttcatatt gctcgtaatt atgcgggcta tggcctgcca caggcggatt
    19081 tgattcagga aggtaacatc ggcctgatga aagcagtgcg ccgttttaac ccggaagtgg
    19141 gtgtgcgcct ggtctccttc gccgtccact ggatcaaagc agagatccac gaatacgttc
    19201 tgcgtaactg gcgtatcgtc aaagttgcga ccaccaaagc gcagcgcaaa ctgttcttca
    19261 acctgcgtaa aaccaagcag cgtctgggct ggtttaatca ggatgaagtt gaaatggtgg
    19321 cccgtgaact gggcgtaacc agcaaagacg tacgtgagat ggagtcgcgt atggcggcac
    19381 aggacatgac ctttgacctg tcttccgatg acgattccga cagccagccg atggctccgg
    19441 tgctctatct gcaggataaa tcatctaact ttgccgacgg cattgaagat gataattggg
    19501 aagagcaagc ggcaaaccgt ctgaccgacg cgatgcaagg tctggacgag cgtagccagg
    19561 atatcatccg cgcgcgctgg ctggatgaag acaacaagtc cacgttgcag gaactggctg
    19621 accgttacgg tgtttccgct gagcgtgtgc gccagctgga aaagaatgcg atgaaaaaac
    19681 ttcgcgctgc cattgaagcg taatttccgc tactcagcag agaaccctgg atgagaatcc
    19741 ggggtttttg ttttttgggc ctctgcaata atcagtttcc ccctccggca aaacgccaat
    19801 ccccacgcag attgttaata aactgtcaaa atagctattc caatatcata aaaatcgggt
    19861 atgttttagc agagtatgct gctaaagcac gggtagtcat gcataaaacg aaataaagtg
    19921 ctgaacaaca acatcacaac acacgtaata accagaagaa tggggattct caggatgaac
    19981 ataaagggta aagcgttact ggcaggatgt atcgcgctgg cattcagcaa tatggctctg
    20041 gcagaagata ttaaagtcgc cgtcgtaggc gcaatgtccg gtccggtggc gcagtacggt
    20101 gaccaggagt ttactggcgc agagcaggcg gttgcggata tcaatgctaa aggtggtatc
    20161 aaaggcaaca aactgcaaat cgtaaaatat gacgatgcct gtgacccgaa acaggcggtt
    20221 gcggtggcga acaaagtcgt taacgacggc attaaatacg tgattggtca cctctgctct
    20281 tcatcaacgc agccagcgtc tgacatctac gaagacgaag gcattttaat gatcacccca
    20341 gcggcaaccg caccagagct gaccgcccgt ggctatcagc tgatcctgcg caccaccggc
    20401 ctggactccg accaggggcc gacggcggcg aaatatattc ttgagaaagt gaaaccgcag
    20461 cgtattgcta tcgttcacga caaacagcaa tacggcgaag gtctggcgcg agcggtgcag
    20521 gacggcctga agaaaggcaa tgctaacgtg gtgttctttg atggtatcac cgccggggaa
    20581 aaagatttct caacgctggt ggcgcgtctg aagaaagaga atatcgactt cgtttactac
    20641 ggcggttatc acccggaaat ggggcaaatc ctgcgtcagg cacgcgcggc agggctgaaa
    20701 actcagttta tggggccgga aggcgtggct aacgtctcgc tgtctaacat tgcgggtgaa
    20761 tcagcggaag gactgctggt gaccaagccg aagaactacg atcaggttcc ggcgaacaaa
    20821 cccattgttg acgcgatcaa agcgaagaaa caggacccaa gcggcgcgtt cgtctggacc
    20881 acctacgccg cgctgcaatc tttgcaggtg ggcctgaatc agtctgacga tccggctgaa
    20941 atagccaaat acctgaaagc gaactccgtg gatacggtga tggggccgct gacctgggat
    21001 gagaaaggtg atctgaaagg ctttgagttc ggcgtatttg actggcacgc caacggcacg
    21061 gcgactgatg cgaagtaatc attaatcggc aactttgggt tgccgccaaa ttgctaatat
    21121 cgagtacgtt gcttcatgcc ggatgcggcg taaacgcctt atccggccta caagattcaa
    21181 agaaatcagt aaattgcagc acacattgta ggcctgataa gcgtagcgca tcaggcaata
    21241 tcacttgtga aatcaaactt gacgattaac gcttctccca gccgccctgt tgtgccgtaa
    21301 atcccagtgc ctgcataaac gctgtcatca cgccgcgatc ttccacgcct gcatccgcca
    21361 tccaccagca tgaaacgcca ggattgttac gcaaaacctc ttccagcaga tattgcccca
    21421 caccgcgacg gcgggtgact tccctcacac gcagggaatc cagtgccccc tcggtgccgc
    21481 ttaaggttac ccgcacggcc gcgagaaggc gctcgttaaa acgcgcggcg tagatacggt
    21541 ggttatcgtc aacctgtaaa gaggaagggg aatactccgg ccagatcttt tgcaggtcaa
    21601 tccggtcctg gtcgctaaag ttttctaatc gaatgatggt cagcttcatg gggaacccgt
    21661 gtaaatcaca aaagtggaac cagtgtagcg aaataattta atcggaggct ttctcttttt
    21721 taattctttt ggcaggtgat taatttttta acagcaataa ttacaaagtt aaaacattag
    21781 agaatgaaaa atacccagca taatcccctg aatggtagtg aattattccg ccctttgtac
    21841 cgttatttta tgctgacaac ggcgcttttt tctgtttatc tatcaataaa ttcagaatat
    21901 tatctgttct taatcgactg aaaaataggg attttaatcg ctatcatcac aaaatactgt
    21961 gctaaaccct taatcagaca ggcaaaaaca gtgcagtata aaaaaagaac agtctgattt
    22021 gttaacacat aagaacaaag caacacaaca tcacgaatgg ggatttttga ctatgaaacg
    22081 gaatgcgaaa actatcatcg cagggatgat tgcactggca atttcacaca ccgctatggc
    22141 tgacgatatt aaagtcgccg ttgtcggcgc gatgtccggc ccgattgccc agtggggcga
    22201 tatggaattt aacggcgcgc gtcaggcgat taaagacatt aatgccaaag ggggaattaa
    22261 aggcgataag ctggttggcg tggaatatga cgacgcctgc gacccgaaac aagccgttgc
    22321 ggtcgccaac aaaatcgtta acgacggcat taaatacgtt attgggcacc tgtgttcctc
    22381 ctctacccaa cctgcatcag atatctacga agacgaaggt attctgatga tttcgccggg
    22441 agcgaccaat ccggagctga cccaacgcgg ttatcaatac atcatgcgta ctgccggtct
    22501 ggactcttcc caggggccaa cggcggcaaa atacattctt gagacggtga agccccagcg
    22561 catcgccatc attcacgaca aacaacagta tggcgaaggg ctggcgcgtt cggtgcagga
    22621 cggattgaaa gcagccaatg ccaacgtcgt cttcttcgac ggcattaccg ctggtgagaa
    22681 agatttctcc gcgctgatcg cccgcctgaa aaaagaaaac atcgacttcg tttactacgg
    22741 cggttactac ccggaaatgg ggcagatgct gcgccaggcc cgttccgttg gcctgaaaac
    22801 tcagtttatg gggccggaag gggtgggtaa tgcgtcgctg tcgaacattg ctggtgatgc
    22861 cgccgaaggt atgttggtca ctatgccaaa acgctatgac caggacccgg caaacaaagg
    22921 catcgttgat gcgctgaaag cagacaagaa agatccgtcc ggaccttatg tctggatcac
    22981 ctacgcggcg gtgcaatctc tggcgactgc ccttgagcgt accggcagcg atgagccgct
    23041 ggcgctggtg aaagatttaa aagctaacgg tgcaaacacc gtgattgggc cgctgaactg
    23101 ggatgaaaaa ggcgatctta agggatttga ttttggtgtc ttccagtggc acgccgacgg
    23161 ttcatcgacg gcagccaagt gatcatccca ccgcccgtga aatgcgggcg gttttagaaa
    23221 ggttacctta tgtctgagca gtttttgtat ttcttgcagc agatgtttaa cggcgtcacg
    23281 ctgggcagta cctacgcgct gatagccatc ggctacacca tggtttacgg cattatcggc
    23341 atgatcaact tcgcccacgg cgaggtttat atgattggta gctatgtctc ctttatgatc
    23401 atcgccgcac tgatgatgat gggcattgat accggctggc tgctggtggc ggcgggattc
    23461 gtcggcgcaa tcgtcattgc cagcgcctac ggctggagta tcgaacgggt agcctatcgt
    23521 ccggtgcgta actctaagcg cctgattgcg cttatttccg caatcggcat gtctatcttc
    23581 ctgcaaaact acgtcagcct gaccgaaggt tcgcgcgacg tggcgctgcc cagcctgttt
    23641 aacggtcagt gggtggtggg ccatagcgaa aacttctctg cctctattac caccatgcag
    23701 gcggtgatat ggattgttac tttcctcgcc atgctggcgc tgacgatttt cattcgctat
    23761 tcccgtatgg ggcgcgcctg ccgtgcctgc gcggaagatc tgaaaatggc gagcctgctt
    23821 ggcatcaaca ccgaccgggt gattgcgctg acctttgtga ttggcgcggc gatggcggca
    23881 gtggcgggcg tgctgctcgg tcagttctac ggcgtaatta acccttacat cggctttatg
    23941 gccgggatga aagcctttac cgctgcggtg ctcggtggga ttggcagcat tccgggggcg
    24001 atgattggcg ggctgatcct ggggattgcg gaggcgctct cttctgccta tctgagtacg
    24061 gaatataaag atgtggtctc attcgccctg ctgatcctgg tgctgctggt gatgccgacc
    24121 ggtattctgg gtcgcccgga ggtagagaaa gtatgaaacc gatgcatatt gcaatggcgc
    24181 tgctctctgc cgctatgttc tttgtgctgg cgggcgtctt tatgggcgtg caactggagc
    24241 tggatggcac caaactggtg gtcgacacgg cttcagagat ccgttggcag tgggtgttta
    24301 tcggcacggc ggtggtcttt ttcttccagc ttgtgcggcc tgctttccag aaagggctaa
    24361 aaagcgtttc cgggccgaag tttattctgc ccgcgattga tggctccacg gtgaagcaga
    24421 aactgttcct cgtggcgctg ctggtgcttg cagtggcgtg gccgtttatg gtgtcgcgtg
    24481 ggacggtgga tatcgccact ctgaccatga tctacattat tctcggtctc gggctgaacg
    24541 tggttgttgg tctttctggt ctgctggtgc tgggatacgg cagcttttac gccatcggcg
    24601 cttacacttt tgcgctgctc aatcactatt acggtctggg cttctggacc tgtctgccga
    24661 ttgccgggtt gatggcggcg gcggcgggct tcctgctcgg ttttccggtg ctgcgtctgc
    24721 gcggtgacta tctggcgatc gttaccctcg ggttcggcga aattgtgcgc atattgctgc
    24781 tcaataacac cgaaattacc ggcggaccga acggtatcag tcagattccg aaaccgacgc
    24841 tcttcggact cgagttcagc cgtaccgccc gcgaaggcgg ctgggacacg ttcagtaatt
    24901 tctttggcct gaaatacgat ccctccgatc gcgtcatctt cctctacctg gtggcgttgc
    24961 tgctggtggt gttaagcctg tttgtcatta accgcctgct gcggatgccg ctgggccgtg
    25021 cgtgggaagc gttgcgtgaa gacgagatcg cctgccgttc gctgggctta agcccgcgtc
    25081 gtatcaagct gactgccttt accatcagcg ccgcgtttgc tggttttgcc ggaacgctgt
    25141 ttgctgcgcg tcagggcttt gtcagcccgg aatccttcac ttttgccgaa tcggcgtttg
    25201 tgctggcgat agtggtgctc ggtggtatgg gttcgcaatt tgcggtgatt ctggcggcaa
    25261 ttttgctggt ggtgtcgcga gagttgatgc gtgatttcaa cgaatacagc atgttaatgc
    25321 tcggtggttt gatggtgctg atgatgatct ggcgtccgca gggcttgctg cccatgacgc
    25381 gcccgcaact caagctgaaa aacggcgcag cgaaaggaga gcaggcatga gtcagccatt
    25441 attatctgtt aacggcctga tgatgcgctt cggcggcctg ctggcggtga ataacgtcaa
    25501 tcttgaactg tacccgcagg agatcgtctc gttaatcggc cctaacggtg ccggaaaaac
    25561 cacggttttt aactgcctga ccggtttcta caaacccacc ggtggcacta ttttactgcg
    25621 cgatcagcat ctggaaggtt tgcccggcca gcaaatcgcc cgcatgggcg tggtgcgcac
    25681 cttccagcat gtgcgtctgt ttcgtgaaat gacggtgatt gaaaacttgc tggtggcgca
    25741 gcatcagcaa ctgaaaaccg ggctgttctc cggcctgttg aaaacgcctt ctttccgccg
    25801 cgcccagagc gaagcgctcg accgtgccgc aacctggctt gagcgcattg gtttgctcga
    25861 acacgctaac cgtcaggcga gtaacctggc ctacggtgac cagcgccgtc tggagatcgc
    25921 ccgctgcatg gtaacgcagc cggagatttt aatgctcgac gaaccggcag caggtcttaa
    25981 cccgaaagag acgaaagagc tggatgagct gattgccgaa ctgcgtaacc atcacaacac
    26041 cactatcttg ttgattgaac acgatatgaa gctggtgatg gggatttcag acagaattta
    26101 cgtggtcaat caggggacgc cgctggcaaa cggtacgccg gagcagatcc gtaataaccc
    26161 ggacgtgatc cgtgcctatt taggtgaggc ataagatgga aaaagtcatg ttgtcctttg
    26221 acaaagttag cgcccactac ggcaaaatcc aggcgctgca tgaggtgagc ctgcatatca
    26281 atcagggcga aattgtcacg ctgattggcg cgaacggggc ggggaaaacc accttgctcg
    26341 gcacgttatg cggcgaccca cgcgccacca gcgggcgaat tgtgtttgat gataaagaca
    26401 ttaccgactg gcagacggcg aaaatcatgc gcgaagcggt ggcgattgtc ccggaaggac
    26461 gtcgcgtctt ctcacgcatg acggtggaag agaatctggc gatgggcggc ttttttgctg
    26521 aacgcgacca gttccaggag cgcataaagt gggtgtatga actgtttcca cgtctgcatg
    26581 aacgccgtgt tcagcgggca ggcaccatgt ccggcggtga acagcagatg ctggcgattg
    26641 gtcgcgcttt gatgagtaac ccacgtttgt tattgcttga tgaaccctcg ctcggactgg
    26701 caccgattat catccagcaa attttcgaca ccatcgagca actgcgcgag caggggatga
    26761 ccatcttcct cgtcgagcag aacgccaacc aggcgctgaa gctcgctgat cgcggctacg
    26821 tgctggaaaa cggtcatgtg gtgctctccg atactggcga tgcgctgctg gcgaacgaag
    26881 cggtgagaag tgcgtattta ggcgggtaat aatattttga gtaaaaagga gtcgaatgac
    26941 tccttttttg tacttgttag ttgtacaatt taattgtact taatatggag gatatcatgc
    27001 ataccatgaa ctacagcgaa gcgcggcaga accttgcatt agttctggaa acagcggcga
    27061 caggtactcc ggtaacgatc acgaggcgtg ggcataaggc agctgtgatt atcagcgcag
    27121 aagagtttga acgttaccag aaggctaaac tggacgcaga atttgacgcc atcatggcta
    27181 ttcatggcag cgaaattaag gagttagcgg ataaatgacc attcagttta tctcagcgga
    27241 ggagataatt tatttccacg ataaattgct tagcgttaca ccaggtgtaa caggaatgtc
    27301 cgatccaggg cgagcagaag cgcttttgta ccgggtactg aataagtatg agtatgaagg
    27361 cgtttctgat ctttggctgc tggcggcgat gcatctattg gcaatctcgc gcggtcatat
    27421 ctttaatgat ggtaataagc gcaccgcact gtttatcacg ctattattcc ttaaacgtaa
    27481 cggcatcata cttgctgcaa acccagagtt tgtagaattg acggttgatg tggcggcggg
    27541 aaaagtatcg ttagaagata tcgttacccg cttgcgtcaa cgcggtacct gacaaggttc
    27601 ttagccattc tgctttttat ccgtgcccca tctcccattt tccctcaccc acgccgtcac
    27661 cgccttgtca tctttctgac accttactat cttacaaatg taacaaaaaa gttatttttc
    27721 tgtaattcga gcatgtcatg ttaccccgcg agcataaaaa gcgtgaattc gcgcatccgg
    27781 tacaacaaga gagataaacg atgaaaccgt tacattatac agcttcagca ctggcgctcg
    27841 gactggcgtt aatggggaat gcacaggcag tgacgaccat tccgttctgg cattctatgg
    27901 aaggggaact gggtaaagag gtggattctc tggcccaacg ttttaacgcc gaaaacccgg
    27961 attacaaaat tgtaccgacc tataaaggca actacgaaca gaatttaagc gcggggattg
    28021 ccgcatttcg taccggcaac gcgccggcta ttttgcaggt ttatgaagtt ggcaccgcca
    28081 ccatgatggc gtcgaaagcc attaaaccgg tatatgacgt gtttaaagag gcggggattc
    28141 agttcgatga gtcgcagttt gtgccgacgg tttcaggcta ctactccgac agcaaaaccg
    28201 gccacttact ctcccagccg ttcaatagct cgactcctgt tctctattac aacaaagacg
    28261 ccttcaagaa agccggttta gacccggaac agccgccgaa aacctggcag gatctggcgg
    28321 actatgccgc gaaactgaaa gcctccggta tgaagtgcgg ctacgccagc ggctggcagg
    28381 gctggatcca actggaaaac tttagcgcct ggaacggtct gccgtttgcc agcaaaaaca
    28441 acggctttga cggcacagac gcggtgctgg agttcaacaa gccggagcag gtgaaacaca
    28501 tcgctatgct cgaagagatg aacaagaagg gcgatttcag ctacgtcggg cgtaaggatg
    28561 aatccactga gaagttctat aacggtgatt gcgcgatgac caccgcctct tccggttcgc
    28621 ttgccaacat tcgtgagtac gccaaattta actacggcgt aggcatgatg ccttacgacg
    28681 ccgatgcgaa agatgcaccg caaaacgcca ttatcggcgg ggccagcctg tgggtgatgc
    28741 agggcaaaga taaagaaacg tataccggcg tggcgaagtt cctcgacttc ctcgcgaagc
    28801 cagaaaacgc tgccgagtgg catcagaaaa ccggttatct gccaatcacc aaagcggcgt
    28861 atgacctgac ccgtgagcag ggcttttatg agaaaaaccc aggggcggat accgcgacgc
    28921 gtcagatgct gaataagccg ccgttaccgt tcaccaaagg gctgcgtctg ggcaacatgc
    28981 cgcagatccg cgtgattgtg gatgaagagc tggagagcgt gtggaccggt aagaagacac
    29041 cgcagcaggc gctggatacc gccgttgagc gtgggaacca gttactgcgc cgctttgagc
    29101 agtctacgaa gtcttaattc tcttaccctc accctaaccc tctcccaaag ggagagggga
    29161 ccgatcggcg tggtatcctg gtgccgtgtc ggtttgcaac ttcgctcggt gggaaagtat
    29221 gcaccgcctt tctctctcgc ccctctgggg agagggccgg ggtgagggga acacaacacg
    29281 aagagtttaa ctatgtcatc atcccgtccg gtgttccgct cgcgctggct gccctatttg
    29341 ctggtcgcgc cgcagctcat catcaccgtt atctttttta tctggcctgc gggcgaagcg
    29401 ttgtggtact cgctgcaaag cgtcgatccg tttggtttct ccagccagtt tgtcggcctg
    29461 gataacttcg tcacgctgtt tcatgacagc tactatctcg actccttctg gacgacgata
    29521 aaattcagca cctttgtcac cgtcagcggt ttgctggtgt cgctgttctt tgcggcgctg
    29581 gtggagtaca tcgtgcgcgg cagccgtttc tatcaaacct taatgctgct gccttacgct
    29641 gtggctcccg ccgttgccgc cgtattgtgg atcttcctgt ttaaccccgg tcgcgggctg
    29701 atcacccatt ttctcgctga gttcggctac gactggaacc acgcgcaaaa cagcggtcag
    29761 gcgatgtttc tggtggtgtt tgcctcggtg tggaagcaaa tcagctacaa ctttctgttc
    29821 ttctatgccg cgctgcaatc cattccccgt tcgttgatcg aagccgcagc catcgacggt
    29881 gcaggcccga ttcgccgctt ctttaagatt gcgctgccgc ttatcgctcc ggtgagtttc
    29941 ttcctgctgg tggtgaacct ggtgtacgcc ttcttcgata ccttcccggt gatcgacgcc
    30001 gccacgtccg gcgggccggt tcaggccacc acgacgctga tttataagat ttaccgcgaa
    30061 ggctttaccg gactggatct ggcttcgtct gccgcgcagt cggtggtgtt gatgttcctc
    30121 gtcatcgtgc tgacggtggt gcagttccgc tatgttgaaa gcaaggtgcg ttaccaatga
    30181 ttgagaaccg tccgtggctg acaatattca gccataccat gctgatcctc gggatcgcgg
    30241 tgatcctctt cccgctgtac gtggcgtttg tcgcggcgac actggataaa caagccgtct
    30301 acgccgcgcc gatgacgcta atccccggca cgcatctgct ggaaaacatc cacaacatct
    30361 gggtgaacgg ggtaggcacg aatagcgcgc cgttctggcg gatgttgctt aacagctttg
    30421 tgatggcgtt cagcattacg ctcggcaaaa ttaccgtctc gatgctctcg gcctttgcca
    30481 ttgtctggtt tcgttttcca ctacgtaacc tcttcttctg gatgattttt atcaccctga
    30541 tgctgccggt tgaagtgcgt atcttcccga cggtggaagt catcgccaac ctgaaaatgc
    30601 tcgacagcta cgcaggttta acgctgccgc tgatggcctc ggcgaccgcc acgtttctgt
    30661 ttcgccagtt ctttatgacc ctaccggatg aactggtgga agccgcgcgg atcgacggcg
    30721 cgtcaccaat gcgtttcttt tgcgacatcg tttttccgct gtccaaaacc aatctggcgg
    30781 cgctgtttgt gatcaccttt atctacggct ggaaccagta tttgtggccg ttgttgatta
    30841 ttaccgacgt tgatctcggc accaccgtgg cagggattaa agggatgatc gccacaggcg
    30901 aaggcacaac ggaatggaac tcggtgatgg cggcgatgtt gttaaccctg atcccaccag
    30961 tagtgattgt tttagtgatg cagcgtgcct tcgtgcgcgg cctggtcgat agtgagaaat
    31021 aagatggcag gactgaaatt acaggcagta accaaaagct gggatggcaa aacccaggtc
    31081 attaaaccgc tgacccttga tgtggcggat ggcgaattta tcgtgatggt cgggccgtct
    31141 ggctgcggga aatcgacgtt attgcgaatg gttgccggac tggagcgggt aacgacgggc
    31201 gatatctgga tcgaccgcaa gcgtgtcacc gaaatggagc cgaaagatcg cgggattgcg
    31261 atggtgttcc agaactacgc gctttatccg catatgagcg tcgaagaaaa catggcgtgg
    31321 gggctgaaaa ttcgcggcat gggcaagcag caaattgccg agcgcgttaa agaagcggcg
    31381 cgcattctgg agctggacgg cctgcttaag cgccgcccgc gtgagctatc tggcggtcag
    31441 cgtcagcgtg tggcgatggg gcgtgcgatt gtgcgtgagc cggcagtgtt cctgtttgat
    31501 gagccgctct ctaacctcga tgccaagttg cgtgtgcaga tgcgtcttga actgcaacag
    31561 ctgcaccgtc gcctgaaaac gacttcactc tacgttaccc acgatcaggt tgaggcgatg
    31621 acgctcgccc agcgagtaat ggtgatgaac ggcggcgttg ccgaacagat tggcacgcca
    31681 gttgaagtgt acgaaaagcc cgccagcctg tttgtggcga gttttatcgg cagtccggcg
    31741 atgaatctgc tgaaagggcg tgtgaataac gaaggtacgc atttcgaact ggacggcggt
    31801 atcgcgctgc cgctaaacgg tggctaccgt cagtatgctg gacgtaaaat gactctcggc
    31861 attcgcccgg aacatattac gctacgttcg caggctgaag gtggcgtgcc gctggtgatg
    31921 gacacgctgg agatcctcgg cgcagataac ctggcgcacg gacgctgggg cgaacagaag
    31981 ctggtgttgc gactggcgca tcaggagcgc ccgacggcag gcagcacgct gtggctgcat
    32041 ctgccggaaa atcagctaca tctttttgat ggtgaaacag gacaacgagt atgagtaact
    32101 ggccttatcc ccgcatcgtc gcccatcgtg gcggcggtaa actggccccg gaaaacaccc
    32161 tggcggcaat cgatgtgggg gcaagatacg gtcataagat gatcgaattt gacgcgaagt
    32221 tatcgaaaga tggcgagatc ttcttgcttc atgacgacaa tctcgaacgc accagcaacg
    32281 gctggggcgt cgcgggtgaa ctgaactggc aggatttact gcgcgtggat gcgggcagtt
    32341 ggtacagcaa agcgtttaaa ggtgagccgc tgccgttgct ttcgcaggtg gcggaacgct
    32401 gtcgcgaaca cgggatgatg gcgaatatcg aaatcaaacc caccactggc accgggccat
    32461 taacgggcaa aacggtggcg ctggcggcac gcgaactgtg gaccggtatg acgccgccgc
    32521 tgctgtcatc gtttgagatt gatgctttag aagccgcgca acaggcagca ccggaactgc
    32581 cgcgtggttt attgcttgat gaatggcgcg acgactggcg agaattgacc gcgcggctgg
    32641 gctgcgtctc tattcatctc aatcataagt tgctcgataa agcgcgagtg atgcagttga
    32701 aagacgccgg attacggatt ctggtttata ccgtcaataa accccagcgc gcggcagagt
    32761 tgctgcgctg gggcgtggat tgcatctgta ccgatgcgat tgacgtgatt ggtccgaact
    32821 ttacggccca atagttttca acggaatgtc aggctgcggc gtgctgggct gattcagcat
    32881 gtcgccgttt ctcctttccg gcagcatatg ctgctgattc aacgaactat ccggattacg
    32941 gttgctgttt aacatcccgc cattggtgtt gggcagcatt tgctgtcggg cgggatttcg
    33001 ctcccccggc tgtgactgca acacccgctg agaattattg tttatctggt tttctaaatg
    33061 ctgctgttgc agctgcgttt gcgttttcag ttgctgattc agcatccctt tttgctggat
    33121 ttgctgcgtt tgcatttggg tttgcatccg ctgctgactg gggatctgat accccggctg
    33181 gttagggtta ttcagagtat taatgggctg tgcaaagccg acaaacggca ggagtgccgt
    33241 aagaatcaga agtcgtttca tcgcgtatcc tcctctgaag atattcttta agtttactcc
    33301 tttcctgcaa tcatcaggat taattcagag ttatatacca ggcttagctg gggttgcccc
    33361 ttaatctctg gagaataacg atgataaaac cgacgttttt acgccgggtg gccattgctg
    33421 ctctgttcac aggaagttgt tttagtacgg tagccgcgcc acctgcgccg tctcctgttt
    33481 cttacggcgt ggaggaagat gtctttcatc cggtacgcgc gaaacagggg atggtggcgt
    33541 ctgtagatgc tactgccact caggtggggg tggatatcct caaggagggc gggaatgccg
    33601 ttgatgccgc cgtggctgtg ggctacgcgc tggcggtaac gcatccgcag gcagggaatc
    33661 tgggcggtgg tggttttatg ctgatccgct cgaaaaatgg caataccacc gctatcgatt
    33721 tccgcgaaat ggctcccgcc aaagcgaccc gcgatatgtt ccttgatgat cagggtaacc
    33781 cggacagcaa aaaatcactc acttcgcatc tggcttctgg tacgcccggt acggtagcag
    33841 gtttctcgct ggcgttggat aaatacggca ccatgccgtt gaacaaagtg gtgcaaccgg
    33901 cgtttaaact ggcacgcgat ggttttatcg ttaacgacgc gttggctgac gatctcaaaa
    33961 cctacggtag cgaagtgttg ccgaatcacg aaaacagcaa ggctatcttc tggaaagagg
    34021 gcgcgccgct gaaaaagggc gacaagctgg tgcaggcgaa cctggcaaag agcctggaga
    34081 tgattgccga aaacggcccg gacgaattct acaaaggcac gatagcggaa cagattgccc
    34141 aggagatgca gaaaaacggt ggcttgatca ctaaagaaga tttagcggcg tataaagcgg
    34201 tcgaacgtac accgattagc ggcgaatatc gcgggtatca ggtttactcg atgccaccgc
    34261 catcttccgg tgggattcat atcgtgcaga tcctcaatat cctggaaaac ttcgatatgc
    34321 agaaatacgg ctttggcagc gccgatgcga tgcaaatcat ggcagaagcg gagaaatacg
    34381 cctacgccga ccgctcggaa tatcttggcg atccggattt tgtcaaagtc ccgtggcagg
    34441 cgctgaccaa taaagcctat gccaaatcta ttgccgatca aatcgatatc aataaagcga
    34501 agccatccag cgaaattcgc cctggcaagc ttgcgcctta tgagagtaat caaactaccc
    34561 attactcagt ggtggataaa gatggtaacg cggtggcggt gacctatacg ctgaacacca
    34621 ccttcggtac gggcattgtc gcgggcgaga gcggtattct gcttaataac caaatggatg
    34681 atttctccgc caaaccgggc gtgccgaacg tttacgggct ggtgggtggt gacgctaacg
    34741 cggtcgggcc gaataaacgt ccgctgtcgt cgatgtcacc aaccattgtg gtgaaagacg
    34801 gtaaaacctg gctggtcacc ggaagcccag gcggtagccg gatcatcact accgtgctgc
    34861 aaatggtggt gaatagcatc gattatggta tgaatgtcgc cgaagcgacc aatgcaccgc
    34921 gtttccacca tcagtggttg ccggatgagc tgcgtgtcga gaaaggattt agcccggata
    34981 cgctcaagct gctggaagca aaaggtcaga aagtggcgct gaaagaggcg atgggcagta
    35041 cacaaagcat tatggttggg cctgacggtg agttgtacgg cgcatccgac ccgcgctcag
    35101 tagatgattt aacggcgggg tattaaggtt agcggccctc ttcgtgggaa gagggccgta
    35161 ttgtcagggc aagccgaagg tagccttttt tatttcataa tcctgtagat attcttccag
    35221 cggctttgcc gttccaaggg aatgaatttc accactgtct ttatcaataa taaaaggtgc
    35281 gttaccggct aagcgcgcgg cctcatcacc agattcaaga aattcttgtg attcgaaaca
    35341 gaaataccag ccctggataa aacgtccatg cagagtgatg acgacaggga gatctgcatc
    35401 atcaaggtaa tggttcgctt tcgcgaatgc gtcgtgataa gtaatcataa ttataataaa
    35461 tattattgtt gagcgttata ttattattct tgc
//
LOCUS       DAZGHF010000038        35123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGHF010000038 DAZGHF010000000
VERSION     DAZGHF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.038"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            46..2397
                     /gene="polB"
                     /locus_tag="ACHK3A_001952"
     CDS             46..2397
                     /gene="polB"
                     /locus_tag="ACHK3A_001952"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO8740033.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCEQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGSIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            2561..5467
                     /gene="rapA"
                     /locus_tag="ACHK3A_001953"
     CDS             2561..5467
                     /gene="rapA"
                     /locus_tag="ACHK3A_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO8740034.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            5479..6138
                     /gene="rluA"
                     /locus_tag="ACHK3A_001954"
     CDS             5479..6138
                     /gene="rluA"
                     /locus_tag="ACHK3A_001954"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO8740035.1"
                     /translation="MGMENYNPPQESWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6256..7071)
                     /gene="djlA"
                     /locus_tag="ACHK3A_001955"
     CDS             complement(6256..7071)
                     /gene="djlA"
                     /locus_tag="ACHK3A_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO8740036.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKLQKGFK"
     gene            7326..9680
                     /gene="lptD"
                     /locus_tag="ACHK3A_001956"
     CDS             7326..9680
                     /gene="lptD"
                     /locus_tag="ACHK3A_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO8740037.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            9733..11019
                     /gene="surA"
                     /locus_tag="ACHK3A_001957"
     CDS             9733..11019
                     /gene="surA"
                     /locus_tag="ACHK3A_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO8740038.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11019..12008
                     /gene="pdxA"
                     /locus_tag="ACHK3A_001958"
     CDS             11019..12008
                     /gene="pdxA"
                     /locus_tag="ACHK3A_001958"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO8740039.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            12005..12826
                     /gene="rsmA"
                     /locus_tag="ACHK3A_001959"
     CDS             12005..12826
                     /gene="rsmA"
                     /locus_tag="ACHK3A_001959"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO8740040.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDKLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            12829..13206
                     /gene="apaG"
                     /locus_tag="ACHK3A_001960"
     CDS             12829..13206
                     /gene="apaG"
                     /locus_tag="ACHK3A_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO8740041.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13213..14055
                     /gene="apaH"
                     /locus_tag="ACHK3A_001961"
     CDS             13213..14055
                     /gene="apaH"
                     /locus_tag="ACHK3A_001961"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO8740042.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKHYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14133..14612)
                     /gene="folA"
                     /locus_tag="ACHK3A_001962"
     CDS             complement(14133..14612)
                     /gene="folA"
                     /locus_tag="ACHK3A_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO8740043.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(14804..16666)
                     /gene="kefC"
                     /locus_tag="ACHK3A_001963"
     CDS             complement(14804..16666)
                     /gene="kefC"
                     /locus_tag="ACHK3A_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO8740044.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGAVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSSEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(16659..17189)
                     /gene="kefF"
                     /locus_tag="ACHK3A_001964"
     CDS             complement(16659..17189)
                     /gene="kefF"
                     /locus_tag="ACHK3A_001964"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO8740045.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(17297..18628)
                     /gene="yaaU"
                     /locus_tag="ACHK3A_001965"
     CDS             complement(17297..18628)
                     /gene="yaaU"
                     /locus_tag="ACHK3A_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8740046.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(18687..18974)
                     /gene="fixX"
                     /locus_tag="ACHK3A_001966"
     CDS             complement(18687..18974)
                     /gene="fixX"
                     /locus_tag="ACHK3A_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO8740047.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(18971..20257)
                     /gene="fixC"
                     /locus_tag="ACHK3A_001967"
     CDS             complement(18971..20257)
                     /gene="fixC"
                     /locus_tag="ACHK3A_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8740048.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(20308..21249)
                     /gene="fixB"
                     /locus_tag="ACHK3A_001968"
     CDS             complement(20308..21249)
                     /gene="fixB"
                     /locus_tag="ACHK3A_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO8740049.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(21264..22034)
                     /gene="fixA"
                     /locus_tag="ACHK3A_001969"
     CDS             complement(21264..22034)
                     /gene="fixA"
                     /locus_tag="ACHK3A_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO8740050.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            22507..24021
                     /gene="caiT"
                     /locus_tag="ACHK3A_001970"
     CDS             22507..24021
                     /gene="caiT"
                     /locus_tag="ACHK3A_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO8740051.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            24052..25194
                     /gene="caiA"
                     /locus_tag="ACHK3A_001971"
     CDS             24052..25194
                     /gene="caiA"
                     /locus_tag="ACHK3A_001971"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO8740052.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            25322..26539
                     /gene="caiB"
                     /locus_tag="ACHK3A_001972"
     CDS             25322..26539
                     /gene="caiB"
                     /locus_tag="ACHK3A_001972"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO8740053.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            26612..28165
                     /gene="caiC"
                     /locus_tag="ACHK3A_001973"
     CDS             26612..28165
                     /gene="caiC"
                     /locus_tag="ACHK3A_001973"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO8740054.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYVLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQRKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            28274..29059
                     /gene="caiD"
                     /locus_tag="ACHK3A_001974"
     CDS             28274..29059
                     /gene="caiD"
                     /locus_tag="ACHK3A_001974"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO8740055.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEAQRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            29065..29655
                     /gene="caiE"
                     /locus_tag="ACHK3A_001975"
     CDS             29065..29655
                     /gene="caiE"
                     /locus_tag="ACHK3A_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO8740056.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAIIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYK
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(29774..30169)
                     /gene="caiF"
                     /locus_tag="ACHK3A_001976"
     CDS             complement(29774..30169)
                     /gene="caiF"
                     /locus_tag="ACHK3A_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO8740057.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(30430..33651)
                     /gene="carB"
                     /locus_tag="ACHK3A_001977"
     CDS             complement(30430..33651)
                     /gene="carB"
                     /locus_tag="ACHK3A_001977"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO8740058.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(33669..34817)
                     /gene="carA"
                     /locus_tag="ACHK3A_001978"
     CDS             complement(33669..34817)
                     /gene="carA"
                     /locus_tag="ACHK3A_001978"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO8740059.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         8422 a         9489 c         9184 g         8028 t
ORIGIN      
        1 gaaaccaggc tataatcaag cctggttttt tattggattt tcagcgtggc gcaggcaggt
       61 tttatcttaa cccgacactg gcgggatacc ccgcaaggaa ccgaagtttc cttttggtta
      121 gcgacggaca acgggccgtt gcaggttacg cttgcgccgc aagagtccgt ggcgtttatt
      181 cccgccgatc aggttccccg cgcccagcat attttgcagg gtgagcaagg ctttcgcctg
      241 acgccgctgg cgttaaagga ttttcatcgc cagccggtgt atggccttta ctgtcgcgcc
      301 catcgccagt tgatgaatta cgaaaagcgc ctgcgtgaag gtggcgttac cgtctacgag
      361 gccgatgtgc gcccgccaga acgctatctg atggagcggt ttatcacctc accggtatgg
      421 gtcgagggtg atatacgcaa tggcgctatc gttaatgccc gtctgaaacc gcatcccgac
      481 taccgtccgc cgctcaagtg ggtttctatt gatattgaaa ccacccgcca cggtgagctg
      541 tactgcatcg gcctggaagg ttgcgagcag cgcatcgttt atatgctggg gccagagaat
      601 ggcgacgcct ccgcgctcga ttttgaactg gagtacgtcg ccagccgccc gcagctactg
      661 gaaaaactca acgcctggtt tgcgactcac gatcccgatg tgatcatcgg ctggaacgtg
      721 gtgcagttcg atctgcgaat gctgcaaaaa catgccgagc gttaccgtat tccgctgcgg
      781 ctggggcgcg ataacagcga gctggagtgg cgcgagcacg gctttaaaaa cggtgtgttt
      841 tttgctcagg ttaaaggtcg gctaattatc gacggtatcg aggcactgaa atccgcgttc
      901 tggaatttct cttcattctc gctggaaacc gtcgctcagg agttattagg cgaaggaaaa
      961 tctatcgata acccgtggga tcgaatggac gaaattgacc gccgtttcgc cgaagataag
     1021 cctgcgctgg caacttataa cctgaaagat tgcgagctgg tgacgcagat cttccacaaa
     1081 actgaaatca tgccgttttt gctcgaacgg gcgacggtga acggcctgcc ggtagatcga
     1141 cacggcggtt cggtggcagc gtttggtcat ctctattttc cccgtatgca tcgcgctggt
     1201 tatgtcgcgc ctaatctcgg cgaagtgccg ccgcacgcca gccctggcgg ctacgtgatg
     1261 gattcgcgac ctgggcttta tgattcggtg ctggtgctgg actacaaaag cctgtacccg
     1321 tcgatcatcc gcacctttct gattgatccc gtcgggctgg tggaaggcat ggcgcagcct
     1381 gatccagagc acagcaccga aggctttctc gatgcctggt tctcgcgaga aaaacattgc
     1441 ctgccggaga ttgtgactaa catctggcac gggcgcgatg aagccaaacg tcagggtaat
     1501 aagccgcttt cgcaggcgct gaagatcatt atgaatgcct tttatggcgt gcttggcacc
     1561 accgcctgcc gcttcttcga tccgcgcctg gcgtcgtcga tcactatgcg tggtcatcag
     1621 atcatgcggc aaaccaaagc gttgattgaa gcgcagggct acgacgtgat ctacggcgat
     1681 accgactcaa cgtttgtctg gttgaaaggc gcacattcgg aagaagaagc ggcgaaaatt
     1741 ggtcgtgcac tggtgcagca cgttaacgcc tggtgggcag aaacgctgca aaaacaacgg
     1801 ctaaccagcg cactggaact ggagtatgaa acccatttct gccgttttct gatgccaacc
     1861 attcgcggag ccgataccgg cagcaaaaag cgctacgccg gactgattca ggagggcgac
     1921 aaacagcgga tggtatttaa agggctggaa accgtgcgca ccgactggac gccgctggcc
     1981 cagcagtttc agcaggagct gtacctgcgt atcttccgca acgagccata tcaggaatac
     2041 gtgcgcgaaa ccatcgacaa gctgatggcg ggcgaactgg atgcgcggct ggtttaccgt
     2101 aaacgccttc gccgtccgct gagcgaatat cagcgtaatg ttccgcctca tgtacgcgcc
     2161 gctcgccttg ccgatgaaga aaaccaaaag cgcggtcgcc ccttgcaata tcagaaccgc
     2221 ggctccatta agtacgtatg gaccaccaac ggcccggagc cgctggacta ccaacgttca
     2281 ccgctggatt acgaacacta tctgacccgc cagctacaac ccgtggcgga gggaatactc
     2341 ccttttattg aggataattt tgctacactt atgaccgggc aacttgggct attttgagca
     2401 aaaaaagagt tcgccagata ccattttgat gcgtgacgaa tgctttgcca tccagtacca
     2461 tagcgccctt tccattcctg gacctgaata acaccactac ctcataagca cggtagcggg
     2521 tggttattgc ctgcaattaa agatatagag ccgaacacat atgcctttta cacttggtca
     2581 acgctggatc agcgatacag aaagcgaatt gggacttgga accgttgtcg cggtggatgc
     2641 gcgaactgtc actttacttt tcccatctac tggtgaaaac cgtctgtacg cacgcagtga
     2701 ttcccccgtg acccgcgtga tgttcaaccc tggtgatact attaccagcc atgacggctg
     2761 gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg ctgacctata tcggtactcg
     2821 cctggatact gaagagtccg gcgtagccct gcgtgaagtt ttccttgata gcaaactggt
     2881 gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt gaccgtatgg accgctttgc
     2941 gctgcgttat cgcgcgcgta agtattccag cgaacagttc cgtatgccgt acagcggcct
     3001 gcgcggtcag cgtaccagcc tgatcccgca ccagctcaac atcgctcatg atgttggccg
     3061 ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt ttagggaaaa ccattgaagc
     3121 cgggatgatc ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc tgattatcgt
     3181 cccggaaacc ttacagcatc agtggctggt agaaatgctg cgccgtttca acctgcgctt
     3241 tgctctgttt gatgatgagc gttatgccga agctcagcac gatgcttaca acccgttcga
     3301 taccgaacag ctggtgattt gctcgctgga ttttgctcgt cgtagcaaac agcgtctgga
     3361 acatctctgt gaagccgaat gggatctgct ggtggtcgat gaagcccatc acctggtgtg
     3421 gagcgaagat gcgccgagcc gcgaatatca ggccattgaa caactggcag agcacgtgcc
     3481 gggcgttctg ctgctgaccg caaccccgga acagctgggg atggaaagcc acttcgcccg
     3541 tctgcgtctg ctggacccga accgtttcca cgattttgcg caattcgttg aagagcagaa
     3601 aaattatcgt ccggttgcgg acgccgttgc catgctgctg gcaggtaaca aactgagcaa
     3661 tgatgaactg aacatgctcg gtgagatgat cggcgagcag gatatcgagc cgctgttgca
     3721 agcagcaaac agcgacagcg aagatgccca gagcgcccgt caggaactgg tttcgatgct
     3781 gatggatcgc cacggcacca gccgcgtgct gttccgtaac acgcgtaacg gtgtgaaagg
     3841 cttcccgaaa cgcgagctgc atactattaa gctgccactg ccgacgcagt atcagaccgc
     3901 tattaaagtc tccggcatta tgggcgcacg taaaagtgcg gaagatcgcg ctcgcgatat
     3961 gctctacccg gagcgtattt atcaggaatt tgaaggcgat aacgccacct ggtggaactt
     4021 cgatccgcgc gttgaatggc tgatgggcta cctgaccagc catcgctctc agaaagtgct
     4081 ggtgatctgc gccaaagctg ccactgcgct gcaactggag caagtactgc gcgaacgtga
     4141 aggtatccgc gctgctgtgt tccacgaagg catgtcgatt atcgaacgtg accgcgctgc
     4201 cgcctggttt gctgaagaag acaccggtgc gcaggtactg ctgtgctcgg aaatcggttc
     4261 tgaaggacgt aacttccagt tcgccagcca catggtgatg tttgacctgc cattcaaccc
     4321 ggatctgctg gagcagcgta ttggtcgtct ggatcgtatc ggtcaggcgc acgatattca
     4381 gatccatgtg ccttatctgg aaaaaactgc tcagtcggtg ctggtgcgct ggtatcacga
     4441 aggtctggat gcattcgagc acacctgccc gaccggacgc actatttacg atagtgtata
     4501 caacgatctg attaactatc tggcttcacc ggatcaaacc gaaggctttg acgatctgat
     4561 caaaaactgc cgcgagcaac atgaagcgct gaaagcacag ctggaacaag gtcgtgaccg
     4621 cctgctggaa atccattcca acggtggcga aaaagcccag gcactggcag aaagcattga
     4681 agagcaggat gacgatacca acctgatcgc cttcgccatg aacctgttcg atattatcgg
     4741 tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg acgccgtccg atcatatgct
     4801 ggtgccggac ttccctggcc tgtcggaaga tggcatcacc atcaccttcg accgtgaagt
     4861 ggcgctggcg cgtgaagatg cacagtttat tacctgggag catccgctga tccgcaacgg
     4921 tctggatctg atcctttctg gcgataccgg tagcagcacg atttcactgt tgaaaaacaa
     4981 agcgttgccg gtaggtacgc tgttggtgga actgatttat gtggttgaag cccaggctcc
     5041 gaagcagttg cagctcaacc gcttcctgcc accgacgccg gtacgtatgc tgctggataa
     5101 aaacggcaac aacctggcgg cgcaggtaga gtttgaaacc tttaaccgcc agcttaacgc
     5161 ggttaaccgt cacaccggca gcaagctggt taacgccgtg cagcaggatg ttcacgctat
     5221 ccttcagctg ggggaagcgc agatcgagaa atctgcccgt gcattgattg atgccgcacg
     5281 taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt ctggaagcgc tgcgtgcagt
     5341 taacccgaac attcgtgacg acgaactgac cgccattgag agcaaccgtc agcaggtaat
     5401 ggaaagcctg gatcaggcag gctggcgtct ggatgccctg cgtttgatcg ttgtgacgca
     5461 tcagtaacgg agccgaagat ggggatggaa aactacaatc cgccgcagga atcctggctg
     5521 gttgtcctgt atcaggatga ccatattatg gtggtcaaca agccgagcgg tttgttgtct
     5581 gtgccgggtc gtctggaaga gcacaaagac agcgtgatga cgcgcattca gcgtgattat
     5641 ccgcaggcag aatcggtgca tcgtctggat atggctacca gcggcgtgat tgtagtggcg
     5701 ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc gcgagcgcga gcctaaaaaa
     5761 cagtatgtgg cccgcgtctg ggggcatcca tcccctgcgg aaggtctggt ggatctgccg
     5821 ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct gttacgaaac aggtaaacct
     5881 gcgcagacgg agtatgaagt ggtggaatat gcggcggata acacggcaag agtggtgtta
     5941 aaaccgatta ccgggcgttc gcatcagctg cgtgtgcata tgctggcgct gggtcatccg
     6001 attctcggcg atcgttttta tgcatcacca gaagcgagag caatggcacc acgtttgttg
     6061 ctacatgcag agatgctgac gattacccat ccggcgtatg gcaatagtat gacgtttaaa
     6121 gcaccagcgg atttttaata gtcacgctgg cgaagttcgg taggccggag aaggcgttca
     6181 cgccgcatcc ggcaattttg cgccgattgc ctgatgcgac gcgggccgcg tcttatcagg
     6241 cctacacgtt gaaacttatt taaacccttt ctgcagctta atcaattcat aggcctgctg
     6301 aatttcctgc gctttttgct tcgccatctc catcatctca ggcggcaaac ctttcgccac
     6361 cagcttatcg ggatggtgtt cactcatcag cttacggtag gcacgtttga tggtggtcgc
     6421 atcatccgtc ggcttcacgc ccagcacatt acaggcatct tccagcgttg ggccacgctg
     6481 cgcttgctgc cagttaccac cgccagattg ctgctgataa ccgccgccaa actgtgcacc
     6541 gccctgcatc atgcgcaaaa actggtcaaa ctgggcgcgg gagatcccta attcttcagc
     6601 aatgacatac agcacagccc gttcattcgg gtgcagtgaa ccatcagcaa acgccgcctg
     6661 aatctggatc tccagaaaca tacgaattaa gtcaaaacga ccaaagcaga cactgcgaaa
     6721 ctggcgcatc ttttcgcgca gcgggtaatt gtctgatttt cccacccgga acgcgttttg
     6781 cgccgcagta cgggaagcgc catgaaggtt cattcggtcc atcaactggc tggcgatatg
     6841 aatatcagcc tccgtgacgc gacctttgga tttggttaaa tgccccatca cttcaaaagt
     6901 ggtggcaaaa aacagcgcct gacgctcacg ctggttggcg aaccacgcca ttttacggct
     6961 acgggcttta tcaaacatat ggccaattaa caggcctaac actacgcccc aaaagccgcc
     7021 gcccatcagt aaggccacgg ccacgccaat gatttttccc caatactgca tatattcccc
     7081 aaatcgacac acggatatca gggctatctc ccacaatata aaggtgcttt taccgttttc
     7141 cggctgcggt caattgtggg acatcgccta taatttgcat tatcatacct gtcattcaat
     7201 gccgtgccta acaccactga cgctattcgg acaggattaa cactagcgta gagatgacga
     7261 gtacgttagt ctctgaccgt ttgtcacgcg caacgttacc gatgatggaa caataaaatc
     7321 aacgtatgaa aaaacgtatc cccactctcc tggccaccat gattgccacc gccctttata
     7381 gtcaacaggg actggcagcc gacctcgcct cacagtgcat gttgggcgtg ccaagctatg
     7441 accgtcctct ggtacagggc gataccaatg acttacccgt gactatcaat gctgaccacg
     7501 cgaaagggga ctacccggat gacgccgtgt ttactggcag cgtggatatc atgcagggta
     7561 acagccgtct gcaggccgac gaagtgcagc tccatcaaaa agaggcgcca ggacaaccag
     7621 agccggtacg taccgttgat gcgctcggta atgtccatta cgacgataac caggtgatcc
     7681 tcaaagggcc gaaaggctgg gcgaatctga acaccaaaga taccaacgtc tgggagggtg
     7741 attaccagat ggtgggtcgc cagggtcgcg gtaaagcgga cctgatgaaa caacgtggcg
     7801 aaaaccgcta taccattctg gataacggta gctttacctc ctgtctgccg ggttctgaca
     7861 cctggagcgt ggtaggcagc gaaattattc atgaccgcga agaacaagtt gcggagatct
     7921 ggaacgcccg ctttaaggtg ggtccggtac cgatctttta tagcccctat ttgcagttgc
     7981 cggtgggtga caaacgtcgc tctggtttct tgatcccgaa cgccaagtac accaccacca
     8041 actactttga gttctacctg ccatattact ggaacatcgc gccaaatatg gatgccacca
     8101 tcacgccgca ttatatgcat cgtcgtggca atatcatgtg ggagaacgaa ttccgctacc
     8161 tctcccaggc gggcgctggc ttgatggaac tggactatct gccttcagat aaagtctatg
     8221 aagatgaaca cccgaacgat gacagttcac gtcgttggtt attctactgg aaccactccg
     8281 gggtcatgga tcaggtgtgg cgtttcaacg tcgactacac caaggtcagc gatcctagct
     8341 acttcaatga ttttgataac aagtacggtt ccagtactga cggctacgca acgcaaaaat
     8401 tcagcgttgg ctatgcggtg caaaacttca atgccaccgt ttcaaccaag cagttccagg
     8461 tcttcagcga gcagaacacc agtagctact cggcagagcc gcagttagac gttaactact
     8521 accagaatga tgttggtccg tttgatacac gcatttacgg ccaggcagtg cactttgtta
     8581 acaccagaga cgacatgcct gaagcaaccc gtgttcacct ggaaccgacc atcaatttgc
     8641 cgctctctaa taactggggc agcatcaata ccgaagcgaa gttgctggca acccattatc
     8701 agcaaactaa tcttgactgg tataactcca gaaacacaac caagctggac gaatccgtta
     8761 accgcgtaat gccgcaattc aaagttgacg gcaaaatggt ctttgaacgc gatatggaaa
     8821 tgctggcgcc gggttatacc caaacgctgg aaccgcgcgc gcagtattta tacgtgccgt
     8881 atcgcgatca gagcgacatc tataactacg actcgtctct gctgcaatct gactactctg
     8941 gcctgttccg ggaccggact tacggcggtc ttgaccgtat tgcctccgct aaccaggtga
     9001 cgaccggtgt cacatctcgc atatatgatg atgctgccgt tgaacgtttt aatatttccg
     9061 ttggtcaaat ctactatttc acggagtctc gcactggcga tgacaacata acatgggaga
     9121 atgacgacaa aacgggctca ctggtgtggg caggcgatac ttactggcgt atctcagagc
     9181 gttggggatt gcgtggcgga attcagtacg atacacgtct ggataacgta gcgaccagta
     9241 actccagcat tgaataccgt cgggatgaag accgtctggt acagctgaat taccgttacg
     9301 ccagcccgga atatattcag gctacgctgc ctaagtacta ttccactgct gagcaatata
     9361 agaatggtat ttcgcaggta ggtgccgtcg ccagctggcc aattgccgat cgttggtcaa
     9421 ttgttggggc ctactactac gacaccaatg cgaacaagca agccgactct atgttaggtg
     9481 tgcaatacag ctcctgctgc tatgcaattc gcgtcggtta cgagcggaag ctgaacggtt
     9541 gggataacga taaacaacat gcggtatatg acaacgcaat cggctttaac atcgaacttc
     9601 gcggcctgag ctccaactac ggtctgggta cgcaagagat gctgcgttcg aacattctgc
     9661 cgtatcaaaa cactttgtga tttgttgatt taccacgtaa tccgcagtgc ggttaattga
     9721 aatggaaaaa gtatgaagaa ctggaaaacg ctgcttctcg gtatcgccat gatcgcgaat
     9781 accagtttcg ctgcccccca ggtagtcgat aaagtcgcag ccgtcgtcaa taacggcgtc
     9841 gtgctggaaa gcgacgttga tggattaatg caatcggtaa aactgaacgc tgctcaggca
     9901 aggcagcagc ttcctgatga cgcgacgctg cgccaccaaa tcatggaacg tttgatcatg
     9961 gatcaaatca tcctgcagat ggggcagaaa atgggagtga aaatctccga tgagcagctg
    10021 gatcaggcga ttgctaacat cgcgaaacag aacaacatga cgctggatca gatgcgcagc
    10081 cgtctggctt acgatggtct gaactacaac acctatcgta accagatccg caaagagatg
    10141 attatctctg aagtgcgtaa caacgaggtg cgtcgtcgca tcaccatcct gccgcaggaa
    10201 gtcgaatccc tggcgcagca ggtgggtaac caaaacgacg ccagcactga gctgaacctg
    10261 agccacatcc tgatcccgct gccggaaaac ccgacctctg atcaagtgaa cgaagcggaa
    10321 agccaggcgc gtgccattgt cgatcaggcg cgtaacggcg ctgatttcgg taagctggcg
    10381 attgctcatt ctgccgacca gcaggcgctg aacggcggcc agatgggctg gggccgtatt
    10441 caggagttgc cggggatctt cgcccaggca ttaagcaccg cgaagaaagg cgacattgtt
    10501 ggcccgattc gttccggcgt tggcttccat attctgaaag ttaacgacct gcgcggcgaa
    10561 agcaaaaata tctcggtgac cgaagttcat gctcgccata ttctgctgaa accgtcgccg
    10621 atcatgactg acgaacaggc ccgcgtgaaa ctggaacaga ttgctgccga tatcaagagt
    10681 ggtaaaacga cttttgctgc cgcagcgaaa gagttctctc aggatccagg ctctgctaac
    10741 cagggcggtg atctcggttg ggctacacca gatattttcg atccggcctt ccgtgacgcc
    10801 ctgacccgcc tgaacaaagg tcaaatgagt gcaccggttc actcttcatt cggctggcat
    10861 ttaatcgaac tgctggatac ccgtaatgtc gataaaaccg acgctgcgca gaaagatcgt
    10921 gcataccgca tgctgatgaa ccgtaagttc tcggaagaag cagcaagctg gatgcaggaa
    10981 caacgtgcca gcgcctacgt taaaatcctg agcaactaat ggttaaaacc caacgtgttg
    11041 tgatcactcc cggcgagccc gccgggattg gcccggactt agttgtccag cttgcacagc
    11101 gtgagtggcc ggtcgaactg gttgtttgtg ccgatgccac tctccttacc gaccgggcag
    11161 cgatgctcgg tttgccgctc accctccgtc cttattcccc caactcccct gcacaaccgc
    11221 aaactgcggg cacattaacg ctacttcctg tcgcgctacg tgaatctgtc actgcggggc
    11281 agttagcggt tgaaaatggg cattacgtag tggagacgct ggcgcgagcg tgtgatggct
    11341 gtctgaacgg tgaatttgct gcgctgatca caggccccgt gcataaaggc gtcattaacg
    11401 acgcaggcat tccgtttacc ggtcataccg agtttttcga agagcgttcg caggcgaaaa
    11461 aagtggtgat gatgctggcg accgaagaac ttcgcgtggc gctggcaacg acgcatttac
    11521 cgctgcgcga tatcgcagat gctatcaccc ctgcgctttt gcacgaagtg attgctattt
    11581 tgcatcacga tttgcggacc aaatttggta ttgccgaacc gcgcattctg gtctgcgggc
    11641 tgaatccgca cgcgggcgaa ggcggtcata tgggtacgga agagatagac accattattc
    11701 cggtgctcga caagctgcgg gcgcagggga tgaaactcaa cgggccgctg cctgccgata
    11761 ccctgtttca gccgaaatat cttgataacg ccgacgcggt gctggcgatg taccacgatc
    11821 agggtcttcc cgtgctaaaa taccagggct tcgggcgcgg tgtgaacatt acgctgggcc
    11881 tgccctttat tcgcacatca gtggaccacg gtaccgcgct tgaactggcg ggacgtggca
    11941 aagccgatgt cggcagtttt attacggcgc ttaatctcgc catcaaaatg attgttaaca
    12001 cccaatgaat aatcgagtcc accagggcca cttagcccgt aaacgcttcg ggcaaaactt
    12061 tctcaacgat cagttcgtga tcgacagcat tgtttctgcc attaacccgc agaagggcca
    12121 ggcgatggtc gaaatcggcc ccggtctggc ggcattgacc gaaccggtcg gcgaacgtct
    12181 ggataagctc acggtcatcg aacttgaccg cgatctggcg gcacgtctgc aaacgcatcc
    12241 attcttaggc ccgaaactga ctatttatca gcaggatgcg atgaccttta actttggtga
    12301 actggccgag aaaatgggtc agccgctgcg tgttttcggc aacctgcctt ataacatctc
    12361 cacgccgttg atgttccatc tgtttagcta tactgatgcc attgccgaca tgcactttat
    12421 gttgcaaaaa gaggtggtga atcgtctggt tgcaggaccg aacagcaagg cgtatggtcg
    12481 attaagcgtc atggcgcaat actattgcaa cgtgatcccg gtgctggaag taccgccgtc
    12541 agcctttaca ccaccaccca aagtggattc cgccgtcgtg cgcctggttc ctcatgcaac
    12601 gatgcctcac ccggttaaag atgttcgcgt gttgagccgc atcaccaccg aagcctttaa
    12661 ccagcgtcgt aaaaccattc gtaacagcct cggcaacctg tttagcgtcg aggtgttaac
    12721 gggaatgggg atcgacccgg cgatgcgagc ggaaaatatc tctgtcgcgc aatattgcca
    12781 gatggcgaac tatctggcgg agaacgcgcc tttgcaggag agttaacgat gatcaattcg
    12841 ccccgagtgt gtattcaggt tcaaagcgtc tacattgagg cacaatcttc acctgataat
    12901 gaacgttacg tttttgctta taccgtaacc atacgcaatc tggggcgagc gccagtgcag
    12961 ttgttggggc gttactggct gatcaccaat ggcaatggcc gtgaaaccga agtccagggc
    13021 gaaggagtgg ttggcgtcca gccacttatc gcacctggcg aagagtacca gtacaccagc
    13081 ggtgcaatca ttgaaacccc gctgggcacc atgcagggtc actacgaaat gatcgatgaa
    13141 aatggcgtcc ctttcagcat cgacattccc gtattccgac tcgccgttcc cacactcatt
    13201 cattaaaaga atatggcgac ataccttatt ggcgacgttc atggttgtta cgatgaactg
    13261 atcgcattgc tgcataaagt agaatttacc cctgggaaag ataccctctg gctgacgggc
    13321 gatctggtcg cgcgcggccc gggttcgctg gatgttctgc gctatgtgaa atccttaggc
    13381 gacagcgtac gtctggtgct gggcaatcac gatctgcatc tgctggcggt atttgccggg
    13441 atcagccgca ataaaccgaa agatcgcctg acaccgctgc tggaagcgcc ggatgccgac
    13501 gagctgctta actggctgcg tcgccagcct ctgctgcaaa tcgacgaaga gaaaaagctg
    13561 gtgatggccc acgccgggat cacgccacag tgggatctac agaccgccaa agagtgcgcg
    13621 cgcgatgtag aagcggtgct gtcgagcgac tcctacccct tcttccttga tgccatgtac
    13681 ggcgatatgc caaataactg gtcaccggaa ttgcgggggc tgggaagact gcggtttatc
    13741 accaacgcct ttacccgtat gcgtttttgc ttcccgaacg gtcaactgga tatgtacagc
    13801 aaagaatcgc cggaagaggc ccctgcccca ctgaaaccgt ggtttgcgat tcctggccct
    13861 gtcgctgaag agtacagcat cgcctttggt cactgggcat cgctggaagg caaaggtacg
    13921 ccggaaggta tttacgcgct ggatactggc tgctgctggg gtggtacatt aacctgcctg
    13981 cgctgggaag ataaacacta ttttgtccag ccgtcgaacc ggcataagga tttgggcgaa
    14041 gcggcggcgt cttaaacaca gcctgataca ggaaggccgg ataagacgcg accggcgtcg
    14101 catccggcac tagccgtaaa ttctatacaa acttaccgcc gctccagaat ctcaaagcaa
    14161 tagctgtgag agttctgcgc atcagcgtcg tggaattcgc tgaataccga ttcccagtca
    14221 tccggctcgt aatccgggaa atgggtgtcg ccttccactt ctgcgtcgat atgcgtcaga
    14281 tacagttttt gcgctttggg caggaactgc tcataaacgc gaccgccgcc aatcaccatg
    14341 atttctggta cgtcaccaca cgccgcaatg gcttcatcca ccgacttcac ccacgttacg
    14401 cgatcgtccg tacccggttg actgctgagg ataatatttt tgcgtcctgg caacggacga
    14461 ccgatggatt cccaggtatg gcggcccata atcacgggtt tatttaaagt gttgcgttta
    14521 aaccaggcga gatcggcagg caggttccac ggcatggcgt tttccatgcc gataacacga
    14581 tctaccgcta acgccgcaat cagactgatc attgagattt cccgataaaa aaaattgtcg
    14641 ccactatacg taaagcgtaa accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt
    14701 ttatattctg ctggcgagtc cacgctctcc cactggactc gccgcattac aatgaaataa
    14761 aaacaattag ttagctgtaa agtgtgattt acgtcactct ttattaggat gagggtttcg
    14821 tttccggttc atccgccatg ttgccggtat gtttaccttc ttcggttccc tgccagccat
    14881 gtcgttgaat taatgacaga tgttcgcggt cctcggtaat gatctcactt aacatcgcgc
    14941 tggtgcgttt atagaccgcc gcgcgggctt tggtgtcgtt ctcaaccatt gccatctctt
    15001 ccaccatctg aatattaaag cggcggaaca catcggcgcg ttctcgcgct tcatacggcc
    15061 ccagacctaa actttccagt gccagacgcc cggttttcag cgcaccttca aaggtttcac
    15121 gctccggctt ttcaacgcct gcctgacgca aacgaatgta gtggtcgaca tcgcgggcgc
    15181 gggcaataat ctgcaaatgc gggaaatgtt ctttcaccat ctccgtcagt tgcaggttgg
    15241 tttgcggatc gtcgatagcg ttaatcagca cttccgcttt cgccgctccg gcagattcca
    15301 gtaaatccat ccgcgtggca tcgccataaa acactttcat accaaattta cgcaaggttt
    15361 cgatatggtc cggatcgtga tcgagcacca ccattttcac cccgctggag agcagtaaac
    15421 gtccggtgat ctgcccaaaa cgaccgaatc cggcgatgat tacccgcggc tgttcttcgt
    15481 cgatctcatc ggcttcacgc gcctcttcac tagaagattg ctcaaggcga ttgaggatca
    15541 ccagcagaat cggcgttgcc gccatcgaca gcgccaccgc cagggtcagc gatttcgccc
    15601 actccggctc cagcacattc gccatctgcg ccgcaccaaa tactacaaag gcaaactcac
    15661 taccctgccc taatagcacc gcaaaccagc gacgctgttt atttggcacc tgcaacggcc
    15721 gggcaatcag ccacagcatg gcgattttga tgatgaggaa accgagcagc aaaatgacaa
    15781 tgcgcaacgg attttcaagc agcgtgccaa agtctatcga catgccaacg ccgatgaaaa
    15841 acagccccag cagcaaacct ttaaatggtt cgatatcgct ttccagcgca tgacggtatt
    15901 ccgagcttgc cagcagtacg cccgccagaa acgcgcccat cgccatcgac aagccgacct
    15961 cttccagcag caaaccaaag ccaaacacga ggaataacgc cacggcacta aacacttccc
    16021 gcaagccaga gcgggcaaca aaacgcagtg ccggacgcgt gacatagcgc cccagcaata
    16081 ccaccagcac cagcgcaccc gccactttta acgccgagag agcaaatgcg cccatcgtcg
    16141 tcgaggcact gctcgccgcc agtagcggga tcatcgccac cagcgggatc gccgcgatat
    16201 cctggaacag cagcaccgca aaggcactgc gacccatttg cgtcaccatc agattgcgtt
    16261 cattcatcgc ctgcatggca atcgccgtag aggagagcgc cagcgtcatg ccgatcaatt
    16321 ccgcgacctg ccagcgcagc ccaagcaaca tacagaacag ccccagcagg ccgccgcaaa
    16381 tcaccatctg caatgcgccg ccgccgaaca ccgccgcacg cagcttccac agcctttgtg
    16441 gatcgagttc gaggccgata ataaacagca tcagcaccac gccaatctcg gcaaagtgca
    16501 gaatagattc ggcatcggtc accagtcgca gcccccacgg gccaataatg cagccggcga
    16561 tcaggtagcc aagtaccgct cccagaccga gacgtaccgc aatgggaaca atcagcgccg
    16621 ccgaaccgag ataaatcagc gcctgaataa gcgtatggct atccatgatg tgcctcctgc
    16681 cattccagca gacgttgctt atagtgccgc gcctgccctt cgagggtttc gtcgtcacaa
    16741 ataaaggtgc agtgcatggc aaacggtggc agccagttca gcccgcagta gattgccgtc
    16801 gcctgtagcg gctgtgacag cacatcaaag cccggatgcg caccaatctc aaaatggctt
    16861 tccccaccgc cggtcgtcac cgcccacagt aaatgtttgc catgcagcgc cgtgccgccg
    16921 tgaccgtaag cccagccgtg cgagaagact ttatcgatcc aaagtttaag aagcggagga
    16981 atgctgtacc actgcatcgg gtgctgccag acgatcagat cggcgcgaga cagcgcctcc
    17041 tgctcggcgg caatatcgat attgaagtca ggatagagtt gataaagaga gcgaatttcg
    17101 acgccttcca gcgtccttgc ctgttcaagc atccgtttat tcgcatggga atgatgcgga
    17161 tacggatgcg cataaattat aagaatcata ggtagcccgc gtgattacgc ctttgttttc
    17221 ccacaaagtg tagtcatata ttcacgcggc ttatagtcaa taatattgat tcagaaaaga
    17281 gagaaatctg aacaaattac cccattctct gcccgcggat cgtcatattg ctggtctgcg
    17341 ccagtgacat ccctcgagtc tccggggcaa acgctacgga aatcaacaag ccaaacagcg
    17401 agatacccgc ccccattagc atcgtgttac tgataccgta attattgata aagatcggca
    17461 gtgcccaggt cgaaacaata gtgccaatac ggcttaagga cataatcacg cccacggcag
    17521 aggcgcggat atccgtcggg aagagttcat taggatagag ccactgcaaa ttacccggcc
    17581 cgccagagaa aaaggcatac accgcaaatg ccatcactac cagccagatc cccatatccg
    17641 ggatcagccc caaaaccgcc agcgccagcg tcatcatggc aaagctgcca atcaacaatg
    17701 gacgccgtcc ggcagtgttt agccacagca tcggtggaat acagccgagc ataaagaaca
    17761 ggctaatcac cacgttcccc agtgccgcgt ttttgccaac acccaatccc aacaaaccaa
    17821 cgatttgcgg gccaaaggtg tagatggcga acatcgggat cacctggcag gtccagatgg
    17881 cggcaacaaa cagaacaaaa ggaaaatggc ggcgattaaa cagatcgcga aaacgggttt
    17941 gctgcggctg ctcttcatcg aaagccaccg gttcgccaaa cagtttgatc atcatctctt
    18001 cgcactcttt tactcgccct ttgcgtaaca accagcgggg agattcaggc agttcgaatc
    18061 gaccaatcaa aatcaacaaa caggggatcg ctgcgctacc cagcatccag cgccaaccac
    18121 cttccacatc ataaagccag taaccgacca gatcggcgca ggtcgcgccg acataccaca
    18181 tcgcggcaat aaagctgatg gaaaacgccc gctgacgggt actggagaac tcagtgatca
    18241 tcgaagtggc gatgggataa tctgcaccga tgacaatgcc gatgagtacc cgcatcacta
    18301 acagttcgac gggggatgag acaaacatcg tcgccaccga tatcacgccg atggcgatga
    18361 tatcaatgag gaacattttg cgccgtccga ctttatcgga gatatagcca aacagcgatg
    18421 tgccaacgaa cagcccggcg agcgttcccg cgcccagcaa gccaatccag tcagcgtcca
    18481 gtttcagcgc cggagtcagt tgctccagcg ccacgccaat cattaccagt acataaccat
    18541 ccagaaacgg accaccgctt ccccacagca aaatgcggcg gtgaatagag gagaatttaa
    18601 gatcatcaaa gtttctggac ggttgcatgg gttattcctg tttttttgca atgagagtca
    18661 gtacggcatt acgtatcaaa ccaacatcag ccgtaacgga actccacacc aaaggtgccg
    18721 cgcgggtatt cccactgttc cagcgccgac cccagcccca gaatgcgaca ggtgccgcac
    18781 tccagacatc cggcgtaatc gaagcgcaca ctgccgtcat cctgcttctt gtacagacct
    18841 gcggggcacg ctttcaccag cagctccagg gcctgtttat cggcatcggc cttcacaacg
    18901 atgtgcggat gctcttcatc gacattgaat ttattgacgc ccagtttgac gtccacattg
    18961 acgggagaag tcataaaacg gtcactcctt tcatgccatc cttgatcaga ttgatgaagc
    19021 ccactttctt gccgtggcgg aggattttct tgcgcatcag ttccggcgca ctgccgtcaa
    19081 tggtgaacag gtcacgcgcc acgcccaccg ccagttccgg gtagccgcta aacatgcgtg
    19141 ggttatcaag gaacgccggt agtttctggt acatgcgcat atcgcgcagc gggccactct
    19201 caagatgctg acgatattcc gcgagttttt gcttactgaa atcgtcgctt ttcatcgctg
    19261 aaagcacagt ttttgctgct gcttccccgg cggcaatcgc cagatccata ccgcgaatgg
    19321 taaaaccgag gttcatacac attccggcag catcaccggc aatcaatacg ccgtcaccaa
    19381 ccaactccgg cagcatgttg atacctgctt ccggcactac gtgagcggaa tattccacca
    19441 gcttgccgcc cgcgatcagc ggtgcaacgg ccggatgttg tttgaaatct tccagcattt
    19501 gcggcaccga tttttttgcg tcatgcagat gatgcaaacc acaaaccagc cccagcgaca
    19561 gggtgttttc attggtataa agaaagccgc cgcccatcag gccatcagtg ggggaacccg
    19621 caaacaggca agccgcaccc tgattaccct gcaactgaaa acggtcttcg ataaccgact
    19681 tcggtaattc gatcagttcc ttcacgccaa ccgccacatc cgtcggttta acgcgttttg
    19741 ccatccccag cttttcggca aggatggagt tcaccccgtc agcaaggatc accgttttcg
    19801 cttcaattac atcgccatcg gcttctacgc cgacaacttt gccatcgcgc tgtacgaggt
    19861 tatcgacacg aatcccggta attaactgcg cacccgcttc ttcggcctgc tccatcagcc
    19921 aggcatcaaa tttactgcgc aaaacggagt aagaacgctg ggatggtgag gtttcgtcac
    19981 cgttgcagta gtccatagtc atcgcagact tttccgtcat aaacgcgagt ttttcatggg
    20041 tgatcaggcg ttctacgggg gcggagtcag cgaaaccagg aataatgtgt tccagactgt
    20101 gggcatagag acgcccgccg gtgacgttct tggcaccagc ggaattgcct cgctcgataa
    20161 ctaacacttg cgccccttcg cgggcgagca ccagtgcggc aaccgaaccg gcaagccctg
    20221 caccgacgat gatggcgtca aagatatctt cggacataag agctccctgt cagcggcagg
    20281 gccaaaatgc agccctgcca gagtggatca acgcgctaaa gctgcggtca gtgccggaag
    20341 gatcttcacg gcgtcgccaa caatgccgta atccgcgtac tggaagatcg gcgcattttt
    20401 atctttgttg atggcgaaaa tggtttgcga cgcattagcg ccaaccatgt gctggatctg
    20461 cccggagatc cccaccgcca ggtacagttc aggtttcagc atcaggttgg agataccgac
    20521 atagcgttcg tgctccatcc atttttcgtt ttccgccacc ggacgagaac aggccaactc
    20581 cgcacctatc gccttgcaaa gctgttctgc cagcgcaatg ttctctttgc tgccaatacc
    20641 gcgaccaacg ctgaccacca ggcgggcttt gtcgagatcg acgctgttgc tctgacgcgc
    20701 ctgggttgcc gtgcgggtaa tcgccacagc cggagcctgc cactccacgg tgtgcgtttc
    20761 gccagtgcgt gacgcgtctg gctgagccac atcgaacgtg ccgctgctga tggtcagaac
    20821 cgcatacggc gtggcaatgc gttcttcgcc aatcgccaga ccgccgtaaa ccatgtgttt
    20881 cactgtcgct ttaccgtcct gcacgctaac agcgctggca tcgttagaca ccgccgcgtt
    20941 aaggcggtaa cccagtttcg ccgccagtaa tttgccgcga cgggtgtttg gcaacagcac
    21001 cagaccgtct gcgccgtgct ggcgaatagt gtcagccatg acaccggcgt aatcttcgat
    21061 catccgctcg tccggtttgc cgcttaattt ccagacatga ttagcgccga gctggattgc
    21121 ctgtgcgccg tcggcatcat tgaggacgaa cgtgttgatt tgattagcta aagcctgcgc
    21181 accgttcatc agttccggca gacgagaagg ggtatcgctg aatacccaga cttgagaaaa
    21241 cgtgttcata gcatcccctg taattaaatg actttgcgaa gattttcagc aaatgcggcg
    21301 atctgttctt cgccgtcgcc ttcaatcacg atgcgctgac gctcgcgctg tttcggcgcg
    21361 gcaacctgtt gttctgacca ggctgcctct gcgttaaaac cgatatccgc cgccgaccat
    21421 acctggactg gctttttcgc cgcgccgaga atggctttca tcgaaggaat ttgtggggag
    21481 ttgatatcag tggaaacagc aacaaccgca ggcagcggaa tgcttaaggt ttctgtttca
    21541 tcttccagtt cgcgctcaac ggtgagggta tctgcagtca gggagataat tttgctgacg
    21601 ccgttaactg ccggaatatt aaggatttcg cctaccagca aaccaacctg ctgggcataa
    21661 aggtcggaag aaccatcgcc acagaggatc aaatcaaagc ctgctttctg agcggctgca
    21721 gccagtacgc tcgccgtttg ttgcggcagt gcctgctcga actggtcatc aatcaccaca
    21781 atcagttcat ccggaccgcg cgatagcaca tctttacgcc ctttggcgtt ggtcagggct
    21841 ttaccgccca cacttaaggc cgtcacctgc gcctctgctg cctgttgctt tagctggcaa
    21901 gccgcttcaa tagcgttgag atcgtattgg cttattttgg catcggcttt gctgaagtct
    21961 aacgaaccat cagcattatt gaccgcaata tcctgttcat caggcacgca cttatagcaa
    22021 gtaataatct tcattgcatc tccagaaatc atgaaggaaa cgtcgttcat atcggtggtg
    22081 ttaattacag atatcacgcc ctttaatttg gaaataaaac tttaatcacc aatattgaaa
    22141 atgtcacacg cagataaatc caactttcaa tattgttaag ctcctcacca atattgaaaa
    22201 catggcgtag aaaaaagaaa ttttcaatat tgttttatgg atcaccaata ttgaaagtca
    22261 gatgctattt ttttactcac aacagaacat aacaaactga ttaataatca aaattaacga
    22321 aaaaatgccc tctattcacc cgaaacaaaa atgtgataac aatcacagaa tgcagcttat
    22381 tgaataccca ttatgagtta gccattaacg cgtccacgag gttaataata attatattaa
    22441 atgttaacaa aaataaaaca aacgggaacg caaaataaat gttcgttttc acagtggaat
    22501 taactcatga agaatgaaaa gagaaaaacg ggaatagaac cgaaggtttt ctttccgccg
    22561 ttaataatcg tcggcatact ttgttggctt acagtcagag atctggatgc ggcgaatgtc
    22621 gttattaatg ctgtattcag ttacgtcacc aatgtatggg gatgggcatt tgaatggtat
    22681 atggtggtga tgcttttcgg ctggttctgg ctggtgtttg gcccgtatgc caaaaagcgt
    22741 ttaggtaacg aaccaccaga atttagtacc gccagttgga tctttatgat gttcgcctcc
    22801 tgtacgtctg ctgccgtact gttctgggga tcgattgaga tctactacta catctccacc
    22861 ccgccgtttg gcttagaacc gaactcaaca ggcgcgaaag agttggggct ggcttacagc
    22921 ttgttccact ggggacctct gccgtgggcc acttacagct tcctttcagt cgccttcgct
    22981 tacttcttct ttgtccgcaa aatggaagtg attcgcccca gctccaccct ggtgccgctg
    23041 gtaggtgaaa aacacgccaa agggttgttc ggcactatcg tcgacaactt ctatctcgtc
    23101 gccttgatct tcgcgatggg taccagtctg ggccttgcca cgccgctggt gaccgagtgt
    23161 atgcaatggt tgtttggcat tccgcatacc ctgcaactgg acgctatcat cattacctgc
    23221 tggattatcc tcaacgccat ttgcgtcgct tgcggtctgc aaaaaggggt acgtatcgcc
    23281 agtgacgtgc gtagttacct gagcttcctg atgctgggtt gggtgttcat cgtcagcggt
    23341 gccagcttca tcatgaacta cttcaccgat tcggtgggga tgttgctgat gtatctgccg
    23401 cgcatgttgt tctataccga tcccatcgct aaaggcggct tcccgcaggg ctggaccgtg
    23461 ttctactggg catggtgggt gatttacgct atccagatga gcatcttcct cgcccgcatc
    23521 tcccgtggtc gtaccgtgcg tgaactgtgc ttcggcatgg tgctggggct gacagcgtca
    23581 acctggatcc tgtggactgt actcggtagt aacactctgc tgttgataga taaaaacatc
    23641 atcaacattc caaatctgat cgaacagtac ggtgtggcgc gcgccatcat cgaaacctgg
    23701 gccgctctgc cactcagcac cgccaccatg tggggcttct tcatcctctg ctttattgcc
    23761 accgttacgc tggttaacgc ctgctcttat accctggcga tgtccacttg ccgcgaagta
    23821 cgcgatggtg aagaaccacc gctgctggtg cgtatcggct ggtcaattct ggttggcatt
    23881 atcggtattg ttctgctggc gctcggcggc ctgaaaccga ttcaaaccgc cattatcgcc
    23941 ggaggatgcc cgctgttctt cgtcaacatt atggtgacgc tctcctttat taaagacgcg
    24001 aaacagaact ggaaagatta attaaccccc aaaatatcaa gaggttgaaa gatggatttt
    24061 aatttaaatg atgagcagga actgtttgtc gccggtatcc gcgaactgat ggccagcgaa
    24121 aactgggagg cctattttgc cgagtgcgac cgtgacagcg tctacccgga acgttttgtc
    24181 aaagcactgg cggatatggg tatcgacagt ctgctgattc ctgaagagca cggtggtctt
    24241 gacgcgggtt ttgttactct cgccgccgtg tggatggagc tgggacgtct gggggcacca
    24301 acctatgtgc tgtaccagtt gccgggcggg ttcaatacct tcctgcgcga aggcacacaa
    24361 gagcagatcg acaagattat ggctttccgc ggcaccggta agcagatgtg gaactcagcg
    24421 attaccgaac cgggcgcggg ctccgacgtg ggtagcctga aaacgactta tacccgtaga
    24481 aatggtaaga tttatcttaa tggtagtaag tgttttatta ccagcagcgc ctacaccccg
    24541 tacatcgtgg tgatggcgcg cgacggggct tctccggaca aacctgtcta caccgaatgg
    24601 tttgttgata tgagcaaacc gggcatcaaa gtgaccaaac ttgagaagct cggtctgcgt
    24661 atggatagct gctgtgaaat cacttttgac gacgtggaac tggacgagaa agacatgttc
    24721 ggtcgggaag gtaacggatt taaccgcgtc aaagaagagt tcgaccatga acgtttcctg
    24781 gtagccctga ccaactacgg tacggcgatg tgcgcctttg aagatgcggc gcgctacgcc
    24841 aaccagcgcg tgcagtttgg cgaggctatt ggtcgtttcc agttgattca ggaaaaattc
    24901 gcccacatgg cgatcaaatt aaactccatg aaaaacatgc tgtatgaagc agcgtggaaa
    24961 gcagacaacg gcaccatcac ctctggcgat gcagcgatgt gcaaatactt ctgcgccaat
    25021 gcggcatttg aagtggtgga tagcgcaatg caggtgctgg gcggtgtcgg gattgcgggc
    25081 aaccaccgca tcagccgctt ctggcgtgac ctgcgtgtag accgcgtttc cggaggatct
    25141 gacgaaatgc agatcctgac gctgggtcgc gcggtgctga agcaataccg ttaagtccgt
    25201 gcgctgcctg atgcgacgct gacgcgtctt atcaggccta cgggagcacg aagatggccg
    25261 gataaggcgt ttacgccgca tccggcagtc atgcgctaca taaatttttc aggagctaat
    25321 aatggatcat ctacccatgc cgaaattcgg gccgctggcc ggattacgcg ttgtcttctc
    25381 cggtatcgaa atcgccgggc cgttcgccgg acaaatgttc gcagaatggg gggcggaagt
    25441 tatctggatc gagaacgtcg cctgggccga caccattcgc gttcaaccga actacccgca
    25501 gctctcccgc cgcaatttgc atgcgctgtc gttaaatatt ttcaaagatg aagggcgcga
    25561 agcgtttctg aaattaatgg aaaccaccga tatcttcatc gaagccagta aaggtccggc
    25621 ctttgcccgt cgtggcatta ccgatgaagt cctgtggcag cacaacccaa aactggttat
    25681 cgctcacctt tccggttttg gtcagtatgg caccgaggag tacaccaatc ttccggccta
    25741 taacactatc gcccaggcct tcagtggtta cctgattcag aacggtgatg ttgaccagcc
    25801 aatgcctgcc ttcccgtata ccgccgatta cttttctggc ctgaccgcca ccacggcggc
    25861 gcttgcagca ctgcataaag tgcgtgaaac cggcaaaggc gaaagcatcg acatcgccat
    25921 gtatgaagtg atgctgcgta tgggccagta cttcatgatg gattacttca acggcggcga
    25981 aatgtgcccg cgcatgacca aaggtaaaga tccctactac gccggttgcg gtctgtataa
    26041 atgcgccgac ggctacatcg tgatggaact ggtgggcatt actcaaattg cagagtgctt
    26101 taaagatatt ggcctcgcac acctgctcgg tacgccggaa atcccggaag gcactcagct
    26161 tatccaccgt atcgaatgcc cttacggccc actggtagaa gagaaactcg atgcctggct
    26221 ggcggcacat accatcgcag aagttaaaga acgctttgcc gaactgaata tcgcctgtgc
    26281 caaagtactg accgtaccgg aactggaaag caatccacag tatgtggctc gcgaatcaat
    26341 cactcagtgg caaacgatgg atggtcgcac ctgcaaaggg ccgaatatca tgccgaaatt
    26401 caaaaataac cccggacaaa tctggcgcgg aatgccctca catggcatgg acacggctgc
    26461 tattttgaaa aatatcggct acagcgaaaa cgacattcag gagttggtca gcaaaggtct
    26521 ggccaaagtt gaggactaaa cgcgtggcgt cagtcagctt gcctgactag cgcccgctca
    26581 ataacaaacg aacaaaatgg atagaggtgc aatggatatc attggcggac aacatctacg
    26641 tcaaatgtgg gacgatctgg cggacgttta cggtcataaa acggcgctga tttgtgaatc
    26701 cagcagcgga gtcgttaacc ggtatagtta tcttgagtta aatcaggaga ttaaccgcac
    26761 ggcaaacctg ttttatgtgc tggggattcg caaaggcgac aaggttgctc tacatctcga
    26821 caactgcccg gaatttatct tttgctggtt cgggttggca aaaattggcg cgattatggt
    26881 gccgattaac gcccgcctgt tgcgcgaaga aagcacgtgg atcctgcaaa atagccaggc
    26941 gtgcctgctg gtgaccagtg cgcaatttta tcctatgtat caacagattc agcaggaaga
    27001 tgcctctcag ttgcggcaca tttgcctgac agatatggta cttcccgctg atgatggcgt
    27061 gagttcgttt actcaacgga aaaatcaaca acctgccacc ttgtgctatg caccgccgct
    27121 atcgactgac gatacggcgg aaattctctt cacctccggc accacctccc gaccgaaagg
    27181 ggtggtgata actcattaca acctgcgctt cgctggatat tactccgcct ggcagtgtgc
    27241 actgcgtgac gatgacgtct acctgacggt aatgcctgcg tttcatatcg attgccagtg
    27301 tactgcggcg atggcggcgt tttctgccgg ggccaccttt gtgctggtcg agaaatacag
    27361 cgcccgcgcc ttctggggac aggtgcagaa gtaccgcgcc accattaccg aatgtattcc
    27421 gatgatgatt cgtacgttga tggtgcagcc gccttcagcc aacgatcggc aacactgcct
    27481 gcgggaagtg atgttttatc tcaacctgtc ggagcaggaa aaagacgcgt tttgtgaacg
    27541 cttcagcgtt cgcttgctga cgtcttatgg gatgacggaa accattgtgg gcattatcgg
    27601 cgatcgccct ggcgataaac ggcgctggcc gtcgattggt cgggcgggct tttgctacga
    27661 agcggagatc cgcgacgatc acaatcgtcc cctcccggct ggtgagctcg gtgaaatctg
    27721 tattaaaggc gtaccaggga aaaccatctt caaagagtat tttctcaacc cgaaagccac
    27781 tgcgaaagtg ctggaagccg atggctggct acataccggc gataccggat accgcgacga
    27841 agagggcttt ttttatttcg tcgatcgccg ctgcaatatg atcaaacgcg gtggcgagaa
    27901 tgtctcctgc gtggagctcg aaaatatcat cgccacccat ccaaaaattc aggatatcgt
    27961 ggttgtgggt attaaagatt cgattcgcga tgaagccatc aaagcatttg tggtgctgaa
    28021 tgaaggtgaa acattgagcg aagaggaatt cttctgcttc tgtgaacaga atatggcgaa
    28081 atttaaagtg ccctcttatc tggagatcag aaaagatctg ccacgtaatt gctcggggaa
    28141 aataattaga aagaatctga aataaatgaa acggcaggga actaccctgt cagctaataa
    28201 tcacgctcta aaacaatatg cattttttgc agggatgttg tcgtccctga aaaagcaaaa
    28261 atggagaaaa ggaatgagtg aatcattaca tctgacccgc aatggatcaa ttctggaaat
    28321 tacccttgat cgtccaaaag cgaatgctat tgatgcaaaa accagctttg aaatgggcga
    28381 agtatttcta aatttccgtg acgatccgca attacgtgtc gccattatta ccggtgccgg
    28441 agagaaattc ttttccgcgg gctgggattt aaaagcggca gcagaaggcg aagcaccgga
    28501 tgctgacttt ggcccgggag gttttgcggg attaaccgaa attttcaatc tcgacaaacc
    28561 ggttatcgca gctgtgaacg gctacgcctt tggcggcggt ttcgaactgg cgctggcggc
    28621 agattttatt gtttgtgccg ataatgccag cttcgccctg ccggaagcca aactgggcat
    28681 cgttcctgac agcggcggcg tgctgcgtct gccgaagatc ctgccgcctg ccatcgtcaa
    28741 tgaaatggtg atgaccggca gacgaatggg cgcagaagag gcgcagcgtt gggggatagt
    28801 caaccgcgtg gttagccagg cggaactgat ggataacgcc cgcgaactgg cgcagcagct
    28861 ggttaacagc gctccactgg cgattgcggc gctgaaagag atctaccgca ccaccagcga
    28921 aatgccggta gaagaagcgt atcgctatat tcgcagcggc gtgttgaaac actatccatc
    28981 ggttctgcat tcggaagatg ccattgaagg gccgctggcg tttgccgaga agcgcgatcc
    29041 ggtgtggaaa ggacgttaac aaccgtgagc tattacgcct ttgagggatt aattccggtg
    29101 gttcacccga cggcgtttgt ccatcccagt gcagtattga ttggcgatgt gattgtggga
    29161 gccggtgtct acatcggccc actcgcctca ctgcgtggtg actacgggcg gttgatcgtg
    29221 caagcgggag ccaatattca ggatggctgc attatgcatg gctactgcga cactgacacc
    29281 atcgttgggg aaaacggcca tatcgggcac ggagcgatcc tgcatggttg tgtgattggt
    29341 cgcgatgcac tggtcgggat gaacagcgtg attatggatg gcgcgatcat tggcgaagag
    29401 agcattgttg ccgctatgag ctttgtcaaa gcgggctttc gcggcgagaa acgccagttg
    29461 ttgatgggta cgccagcccg cgctgtacgc agcgtgagtg acgacgagtt acactggaaa
    29521 cggttgaata ccaaagagta taaggatctt gtcgggcgct gtcatgcatc attacatgaa
    29581 acgcagccac tgaggcaaat ggaggaaaat cgcccccgtt tgcaggggac gacggatgtg
    29641 acgccgaaac ggtgatcttt ccggatgcgg cgtaaacgcc ttatccgacc tggctggaaa
    29701 ggaaccgtag gccgtataag atgcgccagc atcgcatccg gcgaccaggt cgcacggtat
    29761 caacgaaacg acattaacga cgcatactct ttgacaacat catctgccac ttctgctcgc
    29821 ggttaagttc cgtgagcgga acggcaggaa tacgtggcgt ctttcttacg ccgactaatt
    29881 tttcccgact gttattgcgc aggcgcgcgt aaatttgctc atcgatatcg accactttaa
    29941 ccagtcgctg acactggcat ccccgccctt ccagcttatt agggatcatc ttaacttcgc
    30001 agcttatttc tgtgacttcc gacagaatat aagtcagggt attaaccgcc ttgctgtgtt
    30061 caatatcgaa atgaatagag atctctcttg ctatcaccca ccgattttca gccatcatcc
    30121 attcggcgat taacaagtag agtggttttt caacatatcc ttcacacata cgaactccag
    30181 tttcacaaat taaaagtctg gctatgactc gttttgcagc aattaaacaa ggccatatta
    30241 aaattgtgga tatatcgata gtatgcaact tcaccgtcga taagtgtgag ctaaatcctg
    30301 cttatgtcat caaaaatatc gattccccaa aagatttact taatgggcaa aaaaaacggt
    30361 cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccat
    30421 gacacgctat tatttgatct gcgcgtgcat ttcctgcacc gaaattactt tttcagtcgc
    30481 atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cgtaatgcac
    30541 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacgacctga
    30601 ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg
    30661 accttcatgc accttgttca ccagacgcgg attaatgcct gcttcgccca gcacaatcgc
    30721 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    30781 cacgcgttct ttatcgcctt cgcgcacgga aagcaacgca cgaccgtgtt tcttcatggt
    30841 ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat
    30901 gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcccg ggaatttatt
    30961 gaacggcagc accacttctt tcaccgagta gtacggcggg ataacttctt tggttacgcc
    31021 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    31081 ggtggctttg gagacgaacg gaacggtacg cgccgcacgc gggttaactt caatcaggta
    31141 gacttcgttg tttttcaccg caaactgcac gttcatcagg ccgcgcacct gcaattcgaa
    31201 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aagtgtaggc
    31261 tggtagcgaa catgcagagt caccggagtg cacgcccgcc tgctcgatgt gctccatgat
    31321 gccgccaatc agcaccattt caccgtcgca gatggcatcc acgtcaactt ctactgcgtc
    31381 atcaaggaag tggtccagca acactggcgc atcgttagat acgctgaccg ctgtctggaa
    31441 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    31501 agacggacgc accaccagcg ggtagccaat ctctttcgct ttctcaaccg ccatttcgat
    31561 ggtggtaacg gtggcgttcg ccggttgttt cagtttcaga cgctcaaccg catgctggaa
    31621 gcgttcacgg tcttccgcac ggtcgatggc atccgggctg gtaccgataa ccggtacgcc
    31681 agcagcttcc agcgcgcgcg ccagtttcag cggggtctga ccgccgtact ggacgataac
    31741 gcctttcggc ttctcaatac gcacaatttc cagcacgtct tccagagtta ccggctcgaa
    31801 gtagagacgg tcagaggtgt cgtagtcggt ggagacggtt tccgggttac agttaaccat
    31861 aatggtttca taaccatctt cgcgcagcgc cagcgaggcg tgtacacagc agtagtcgaa
    31921 ttcgataccc tgaccgatac ggttcgggcc gccgccgagc accatgattt tttcacggtc
    31981 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    32041 ggcgaactct gccgcacagg tatccacgcg cttataaacc gggtgcaagt catactggtc
    32101 gcgcagcttg cggatttctg cttcacgcac gcccgcgagt tttgccagac gcgcgtcggc
    32161 aaagcctttg cgtttcagct ggcgcaggaa gtcagcgttc aggccagtga tgcccacttc
    32221 tgcgactttc tcttccagac gcaccagctc ttcaatttgt accaggaacc agcggtcaat
    32281 gttggtcagg ttgaacacgc cgtctacgga caggcccgcg cggaaagcat cggcgatata
    32341 ccagatacgc tctgcgcctg catctttcag ttcgcggcga attttggtca gcgcttccgg
    32401 atcatcgagg ctgactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc
    32461 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    32521 ctgagtggtc agacggtcgt tcgccccggc gaatttctcg aagttgaagc gaggaatttt
    32581 agtcaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgcccac cggtgatgtc
    32641 gttcatcagc tcgtcgaggg tgtaacccac cgccagtttc gccgccactt tggcaatcgg
    32701 gaaaccagtg gctttcgacg ccagcgcaga ggagcgagac acgcgcgggt tcatttcgat
    32761 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acgttggaac caccggtttc
    32821 aacgccgatt tcacgcagta ccgccatcga ggcgttacgc atgatttgat attctttgtc
    32881 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc
    32941 aaagttttcg atagagcaga caatgatgca gttgtcgttt ttatcacgca ccacttccat
    33001 ctcgtactct ttccagccga tcagcgactc atcaatcagc agctctttag tcggagagag
    33061 atccagacca cgggcgcaaa tttcttcaaa ctcttcgcgg ttataagcga taccgccgcc
    33121 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagccaactt cagcggcaac
    33181 cgccagcgct tcttccattg aatgagcgat accggaacgc gcggtttcca gaccgatttt
    33241 cttcatcgct acgtcgaaac ggcggcggtc ttcggcttta tcaatcgcat cggcagtggc
    33301 accaatcatg gtgacgccga actcttccag cacgccctga cgctcaagct caagcgcaca
    33361 gttcagcgcc gtctgccccc ccatggttgg cagcaccgcg tccggacgct ctttttcaat
    33421 aatcttgcgt accacttccc agtgaatcgg ttcgatgtag gttgcatcag ccatttccgg
    33481 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcacg
    33541 cagggcttta cacgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    33601 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    33661 actcctgatt acttagcggt tttacggtac tgctcaatta actcgataaa gtggtcaaac
    33721 aacggcgcgg cgtcgtgtgg accagggctg gcttccgggt gtccctggaa gctgaacgcc
    33781 ggtttatcgg tacgatgaat gccctgtaac gtaccgtcga acagggattt atgtgtgaca
    33841 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtaatc
    33901 atcaccacat ttttctcaac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    33961 atttttacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag gcagatgccg
    34021 aataccggaa tatcggtttc gaggaatttc tggatggcgg taatggcgta atcgcacggg
    34081 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacgtct
    34141 tccgcagaag tttgcgccgg aacgatggtc agacgacagc ctctatccac caacatgcgc
    34201 aggatgttgc gcttggcacc aaaatcataa gccacaacat ggaacggcag ctcgtcttct
    34261 ttcttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    34321 tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct
    34381 ttttctaatg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    34441 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    34501 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    34561 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    34621 ttagtgccga cattaccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    34681 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    34741 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    34801 agcgctgact taatcaaaac accctccaga gaatattcac tcactttatt tgcatattaa
    34861 ttcacaatga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    34921 ggcattctgg agatatgggc gctaaaagtc aacttaatgg cgggattttt tatattattt
    34981 tgtggctcat gcatgtttta caggcattaa gcgacaaaaa gatcatataa catgttctgg
    35041 aaaacgcttg cgcagcaaga agattgcatg aaatcaaaat gagcagaaaa aagtggacca
    35101 aatggtcaaa atttacatta aaa
//
LOCUS       DAZGHF010000039        34620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGHF010000039 DAZGHF010000000
VERSION     DAZGHF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.039"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(488..754)
                     /gene="minE"
                     /locus_tag="ACHK3A_001979"
     CDS             complement(488..754)
                     /gene="minE"
                     /locus_tag="ACHK3A_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO8740060.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(758..1570)
                     /gene="minD"
                     /locus_tag="ACHK3A_001980"
     CDS             complement(758..1570)
                     /gene="minD"
                     /locus_tag="ACHK3A_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO8740061.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1594..2289)
                     /gene="minC"
                     /locus_tag="ACHK3A_001981"
     CDS             complement(1594..2289)
                     /gene="minC"
                     /locus_tag="ACHK3A_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO8740062.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSAIGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            2808..3176
                     /gene="ycgJ"
                     /locus_tag="ACHK3A_001982"
     CDS             2808..3176
                     /gene="ycgJ"
                     /locus_tag="ACHK3A_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO8740063.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVN
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3279..3680)
                     /gene="pliG"
                     /locus_tag="ACHK3A_001983"
     CDS             complement(3279..3680)
                     /gene="pliG"
                     /locus_tag="ACHK3A_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO8740064.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            3922..4215
                     /locus_tag="ACHK3A_001984"
     CDS             3922..4215
                     /locus_tag="ACHK3A_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO8740065.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4287..4946
                     /gene="ycgM"
                     /locus_tag="ACHK3A_001985"
     CDS             4287..4946
                     /gene="ycgM"
                     /locus_tag="ACHK3A_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO8740066.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5038..5484
                     /gene="ycgN"
                     /locus_tag="ACHK3A_001986"
     CDS             5038..5484
                     /gene="ycgN"
                     /locus_tag="ACHK3A_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO8740067.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(5691..6017)
                     /gene="hlyE"
                     /locus_tag="ACHK3A_001987"
                     /pseudo
     CDS             complement(5691..6017)
                     /gene="hlyE"
                     /locus_tag="ACHK3A_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            6278..6697
                     /gene="umuD"
                     /locus_tag="ACHK3A_001988"
     CDS             6278..6697
                     /gene="umuD"
                     /locus_tag="ACHK3A_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO8740068.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            6697..7965
                     /gene="umuC"
                     /locus_tag="ACHK3A_001989"
     CDS             6697..7965
                     /gene="umuC"
                     /locus_tag="ACHK3A_001989"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO8740069.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(8010..8540)
                     /gene="dsbB"
                     /locus_tag="ACHK3A_001990"
     CDS             complement(8010..8540)
                     /gene="dsbB"
                     /locus_tag="ACHK3A_001990"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO8740070.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(8686..10227)
                     /gene="nhaB"
                     /locus_tag="ACHK3A_001991"
     CDS             complement(8686..10227)
                     /gene="nhaB"
                     /locus_tag="ACHK3A_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO8740071.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            10449..11168
                     /gene="fadR"
                     /locus_tag="ACHK3A_001992"
     CDS             10449..11168
                     /gene="fadR"
                     /locus_tag="ACHK3A_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO8740072.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(11220..12752)
                     /gene="ycgB"
                     /locus_tag="ACHK3A_001993"
     CDS             complement(11220..12752)
                     /gene="ycgB"
                     /locus_tag="ACHK3A_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO8740073.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            13082..14380
                     /gene="dadA"
                     /locus_tag="ACHK3A_001994"
     CDS             13082..14380
                     /gene="dadA"
                     /locus_tag="ACHK3A_001994"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO8740074.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            14390..15460
                     /gene="dadX"
                     /locus_tag="ACHK3A_001995"
     CDS             14390..15460
                     /gene="dadX"
                     /locus_tag="ACHK3A_001995"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO8740075.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPYARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(15517..17253)
                     /gene="cvrA"
                     /locus_tag="ACHK3A_001996"
     CDS             complement(15517..17253)
                     /gene="cvrA"
                     /locus_tag="ACHK3A_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO8740076.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(17348..18262)
                     /gene="ldcA"
                     /locus_tag="ACHK3A_001997"
     CDS             complement(17348..18262)
                     /gene="ldcA"
                     /locus_tag="ACHK3A_001997"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO8740077.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            18362..18973
                     /gene="emtA"
                     /locus_tag="ACHK3A_001998"
     CDS             18362..18973
                     /gene="emtA"
                     /locus_tag="ACHK3A_001998"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO8740078.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18975..19709)
                     /gene="ycgR"
                     /locus_tag="ACHK3A_001999"
     CDS             complement(18975..19709)
                     /gene="ycgR"
                     /locus_tag="ACHK3A_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO8740079.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19910..20164
                     /gene="ymgE"
                     /locus_tag="ACHK3A_002000"
     CDS             19910..20164
                     /gene="ymgE"
                     /locus_tag="ACHK3A_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO8740080.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(20214..22184)
                     /locus_tag="ACHK3A_002001"
     CDS             complement(20214..22184)
                     /locus_tag="ACHK3A_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO8740081.1"
                     /translation="MRLKKRYLCTALSLAFTQQALAAQESDTLTVWSSPVSSTTTTVL
                     DHPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(22210..23064)
                     /gene="modD"
                     /locus_tag="ACHK3A_002002"
     CDS             complement(22210..23064)
                     /gene="modD"
                     /locus_tag="ACHK3A_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO8740082.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     NPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            complement(23061..23873)
                     /locus_tag="ACHK3A_002003"
     CDS             complement(23061..23873)
                     /locus_tag="ACHK3A_002003"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8740083.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVVELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            complement(23883..24641)
                     /locus_tag="ACHK3A_002004"
     CDS             complement(23883..24641)
                     /locus_tag="ACHK3A_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8740084.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGQ
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(24638..25618)
                     /locus_tag="ACHK3A_002005"
     CDS             complement(24638..25618)
                     /locus_tag="ACHK3A_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO8740085.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVTV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(25618..26640)
                     /locus_tag="ACHK3A_002006"
     CDS             complement(25618..26640)
                     /locus_tag="ACHK3A_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO8740086.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCPLSDADY
                     QKLVAS"
     gene            complement(26977..28674)
                     /gene="treA"
                     /locus_tag="ACHK3A_002007"
     CDS             complement(26977..28674)
                     /gene="treA"
                     /locus_tag="ACHK3A_002007"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO8740087.1"
                     /translation="MKSPAASRPLKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQNEAEPTP"
     gene            complement(28995..30413)
                     /gene="dhaM"
                     /locus_tag="ACHK3A_002008"
     CDS             complement(28995..30413)
                     /gene="dhaM"
                     /locus_tag="ACHK3A_002008"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO8740088.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPSYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            complement(30424..31056)
                     /gene="dhaL"
                     /locus_tag="ACHK3A_002009"
     CDS             complement(30424..31056)
                     /gene="dhaL"
                     /locus_tag="ACHK3A_002009"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO8740089.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSIPAALEAASS
                     VAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(31067..32137)
                     /gene="dhaK"
                     /locus_tag="ACHK3A_002010"
     CDS             complement(31067..32137)
                     /gene="dhaK"
                     /locus_tag="ACHK3A_002010"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO8740090.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRSDAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEESQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            32365..34284
                     /gene="dhaR"
                     /locus_tag="ACHK3A_002011"
     CDS             32365..34284
                     /gene="dhaR"
                     /locus_tag="ACHK3A_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO8740091.1"
                     /translation="MSSAFNNDGRDISPLIATSWERCIKLMKRDTWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRACALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQEDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLHSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            complement(34321..34497)
                     /locus_tag="ACHK3A_002012"
     CDS             complement(34321..34497)
                     /locus_tag="ACHK3A_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740092.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
BASE COUNT         8591 a         8533 c         8938 g         8558 t
ORIGIN      
        1 caggcaggta ttaaagcctc attcagcagt tcgttaagcg ggcatctcgg ggtggggtat
       61 agccatggtg ccggtgtgga atccccatgg aacgcggtgg ctggtgcgaa ctggtcgttc
      121 tgaccatcaa cgattaaact gcgcttcgcg cagttttcgt ttaccgggtg ctaaaaggga
      181 aaattctgcg gcaaaaaaag cccgccagtt acggcgggaa acctcatcct atgggagaac
      241 aatgaataat gaaattgcgg ggttatcatc tcccagtata cccatactaa caataaggtt
      301 atttactcag ccaggtataa acaatttgtt ctgtgtgggg ggaacggcat taagatgtaa
      361 agggggggaa gcaatgagta gcatcagcaa gaatactcgc cgctcttatc aaacgtggat
      421 gagggggatg aaaaactcaa ggcagagatg actctgcctt gaagataaat gcgcttttac
      481 agcgggctta tttcagctct tctgcttccg gtaaggtcac gttcagctca agaatagaaa
      541 tatcgccatc tttttgctca agctgtacgg ttaccatctc aggatcaatt tgtacgtatt
      601 tacaaatgac ctcaagaata tctttacgca actgcggcag ataatgcggt tctgcatcac
      661 tgcgacggcg ttcagcaaca ataatctgca gccgttcttt tgcaatgttg gccgtgtttt
      721 tcttccgcga tagaaagaaa tcgagtaatg ccataactta tcctccgaac aagcgtttga
      781 ggaagccttt cttctcttct tcaatgaagc ggaaaggacg ttcttctccc aacagacgtt
      841 ctacggtatc tgcgtaggct ttacccgcat cggcgttaat gtcgagaatg accggttcac
      901 cctggttaga ggcgcgcaat actgattgat cctcagggat cacgccgacg agtttgatgc
      961 gcaggatctc cagcacatct tccatgctca gcatgtcacc tctgcttacg cggcctgggt
     1021 tatagcgcgt taacagcagg tgctctttaa taggctcttc gccattttct gcgcggcgtg
     1081 atttcgacgc cagaatgcct aaaatacggt cagagtcgcg tactgaggag acttccgggt
     1141 tggtggtaat aatggcttcg tctgcaaaat agagtgccat tagcgcaccg gtctcaatcc
     1201 ctgccgggga gtcgcaaaca ataaattcaa agtccatcgc tttcagatca tcaagaactt
     1261 tggcgacccc ttcacgggtg agggcatctt tatcgcgagt ttgcgatgcc ggaagaatat
     1321 agagattttc agtacgctta tctttaatta acgcctgatt tagcgttgca tcgccctgaa
     1381 tgacgttgac gaaatcgtaa acgacccgac gttcacaacc cataatcagg tcgagattac
     1441 gcaggccgat atcaaaatct atcacgacag ttttctttcc cttctgggcc aaaccagtgg
     1501 cgatggccgc gctggaggtt gtcttaccaa cacccccttt gcccgaagta acaacaataa
     1561 tgcgtgccat agaaattcct tgttaaaaag ggatcaattt aacggttgaa cggtcaaagc
     1621 gttttcgact aactgcagtc gcgccgcttt gccataaaat tctgctggga tttgatcact
     1681 cagccagtat tcacctgcga tggacaccag ttccgccatc aggttcgtac aaaatatttg
     1741 cgtttcccgg tcaccacttg cccctgccag cgcacgaccg cgcatcatgc catagacgtg
     1801 aatattacca tcggcaatca attcggcccc agcgctaacg tggcttgtaa caatcagatc
     1861 acattgtgga gcataaatac gctgaccgga acgcaccggg gtatctatta aacgcgtttt
     1921 tgtgaccggc gttgtatttt gcgctggagc ctgcggtgcg ggagctggac gcggcgcttt
     1981 ttcctttcct tccgtcagga taggcagccc catcttttca atttcggctt taagttgcgc
     2041 atctttgcag ccacttacgc caataacccg caaaccgatt gccgaaaccg ccttatgcat
     2101 cgctgaccag tttaccgggt cttccagtgc actgacgttg agtacaacgg gggcatgttt
     2161 taaaaatgcg ggggcctgag cgattttgtc ttccagcgcc tgatggataa ccttaggttc
     2221 tgcctcatgc agatgaacca cagataaagt gaagctactg cctttaagct cgattggcgt
     2281 gtttgacatc ctggccttac tcaattagct attaatcatc gccagcgcgc gatgatgttc
     2341 cgaagactat aaggcatgtt atagtctgga ttatattgag gcaagtcacc ctcccattta
     2401 ttcagagtaa aagtctattc tgtgataaat ggcgctgatt catagcttaa aaaataccct
     2461 tgtcaatcaa cccattgccg tcgtactttt gattgttctt atttacgctt cctttttccg
     2521 caccctaatc gtgacagcat cttcagacct gtttaaatat ccagtattga ggcgagtgta
     2581 ttttttcacc acgccaggct ttcttaactt tccatttttg cagcatcatt aaagaacaga
     2641 tacccgttat cttaatatcc gccacagaat tgctctatca tagaaaccgc agaagacgtc
     2701 tgatacttct gtcaggtcat cgtaactcgt ccaggtcagc ggttttgatt acggattgat
     2761 gatgaaatga gcataagtgt tgtacggttg gattatcagg atgtgctatg aatatgatgc
     2821 gtatttttta tatcggattg tcaggtgtgg ggatgatgtt ctcatcaatg gcgtctggac
     2881 atgacgctgg cggacttcaa tctccggcgt gcggcgttgt ttgcgacccg tatatttgtg
     2941 ttaactcaga tggcatttcc ccagagttaa caaggaaata tctcggcgaa aaagccgctg
     3001 aaaacttaca atcattacaa ggctacgatc ccagcgaatt tacattcgct aacggtgtat
     3061 tttgcgatgt taaagaaaaa ttatgtcgtg atgatcgtta ttttggtgtg aatggtaagc
     3121 ggagtggaaa aatcaatcaa accaccacaa agatgttatt tatgtgtcgt gagtgatgtc
     3181 ttgtttacat tatgttctat tcgtcattaa cctcttgagc gacagagagg agtggggcgt
     3241 taacgtttat gacggggaga gtccccgtcg gttgatattt acttaatttg aatatcgacg
     3301 ttatattttt tagttttgtt tttacgggcg tcgtttcggg tttgcagcac tcgcaactca
     3361 tatttgccag aagcagggag tgagtattgt ccgtgactat cgagttctgg ggaatatctg
     3421 gataaatcaa cagaatcatc gattcctggc ccgaagaggt aagtatcagc gccttcatta
     3481 gagatactca catgcacttt ctgacctttt ttggcataga aggtatatgt atcgtaatcg
     3541 tatcccttta tttcgccgga gtactgagcg ctgctatggc cttttctgaa ctcaacattg
     3601 acattcttac ccgcagcaaa gctcgtggag gttagcagtg caagcaacaa tacagcctta
     3661 ctgatgccct taattttcat tggtagtctc ctgttcagaa tgagtacgat gaactgttat
     3721 aatataacaa ttcatcacag gagaattcca gtggctttcc tgcgttcggt taaatcccgg
     3781 cgcggcattt ttgccggggg gattacttat aaagggggca tggactgaac aaaccgtagg
     3841 aatctacagc tggctggctt gtggcgcggg tttcattgat gttagcttat gccaaaacct
     3901 ggcatcttaa agagtaaaag tatgttttgt gtgatttatc gaagcagcaa gcgtgatcag
     3961 acctatttat atgtcgaaaa aaaagacgat ttttcgcgtg ttcctgagga actgatgaaa
     4021 ggttttggtc agcctcagtt agcgatgatt cttccgctgg atgggcggaa gaaactggtc
     4081 aatgccgata ttgaaaaggt taaacaggca ttaaccgagc aaggttacta tttgcagtta
     4141 ccgccaccac ccgaagattt gctgaagcaa catctttccg tcatggggca gaaaacagac
     4201 gacacgaaca aataaccgat atccggcggt ggcattatct ttgtcggcgc gggttttcat
     4261 atccacgata aggtgagggg aacgttatgt atcaacatca caactggcaa ggtgcgctgc
     4321 tggattatcc ggtgagtaaa gtagtctgtg ttggtagtaa ctatgccaaa catattaaag
     4381 agatgggcag cgcagtgccc gaagagccag tgctgtttat taaaccagaa acggcactgt
     4441 gcgatctgcg gcagccattg gcgatcccat ccgatttcgg ttcagttcat catgaagtcg
     4501 aactggcggt attgattggc gcgacgctgc gtcaggctac ggaagagcat gtccgtaaag
     4561 ccattgccgg ttacggcgtg gcgctcgatc tgacattgcg tgatgttcag ggaaaaatga
     4621 agaaagccgg gcagccgtgg gaaaaggcca aagcgtttga taactcttgt ccgctttccg
     4681 ggtttattcc cgcggcggaa ttcaccggcg atccgcaaaa tacaacgctg ggcctgagcg
     4741 taaacggcga acaacgccag caaggtacga ctgcggacat gatccataaa atcgttccgc
     4801 tgatcgctta tatgagcaag ttctttaccc tcaaggccgg tgacgttgtg ctgacaggca
     4861 cgcctgatgg cgtcggcccg ttgcaaagtg gtgatgagct gacagtcact ttcgatgggc
     4921 attctttgac aactcgcgtt ttgtaatact ttttgccgcc tgaaagcggc ggcaaaactt
     4981 gcatcgctgt gccagactgg ttataaggtg cgttttaacg taatggcgga acacctgatg
     5041 agcgatgtac ctttctggca aagtaaaacc ctggacgaaa tgagcgatgc ggaatgggag
     5101 tcgttgtgtg atggttgcgg tcagtgttgc ctgcataagc tgatggatga agacaccgac
     5161 gaaatctact tcactaacgt cgcctgtcgc cagctcaata ttaaaacctg tcaatgtcgg
     5221 aactacgaac gtcgtttcga gtttgaacct gactgcatta aattaacccg tgaaaatctg
     5281 ccaacattcg aatggctgcc aatgacctgc gcttatcgct tgctggcgga aggtaaagat
     5341 ttacctgcgt ggcatccgct gcttactggt tcgaaagcgg caatgcatgg tgaacgtatc
     5401 tctgtgcgtc atatcgcagt gaaagaatca gaagtgattg actggcagga tcatatctta
     5461 aataaacctg actgggcgca gtgataaatt taatagatgt tgatgggggc tttcttaata
     5521 tacgtctgga tactcgccat ttcgtagccc tgtttttgaa agcgctctgt cataatgaga
     5581 gttacccgat accttattat caacaggtaa ctctcaatct ttaaaaaacg gctgtgtttt
     5641 taaatgtgat aaaccttata ccttgagtac atggagagaa taatcgctta tcagacttca
     5701 ggtacctcaa agagtgtctt tttaccgtgt cttttctgat actcattaca ggtgttaatc
     5761 atttttttgg ccgcttcttt tagcaaagaa agcattaaat catcataatc aacatagaat
     5821 ctggttgttt cagtttccgt ttttatctcc ccgatggcgg ctatttcggt ggttaatttc
     5881 aatttggcgg catcgatatc tttattcgct tgtttaaccg tgttagacag ggtggtaaag
     5941 aaactctgca cagattttaa cttgttcttc aattctggaa tcagtttccc ttcaactacg
     6001 cccgctgcaa cagaatacag tttcttgatg ccgtcatcca gtaccttaat gagaatgcct
     6061 ttctgggcga atgcttctat tctatttttt agccgggcga tatttttcat ttctgctgga
     6121 tgagcgtcgt cgccagaagg ccacgtgagc acaggataag agaacgaaaa atcagcagtc
     6181 tatgcagcga caaatattga tagcctgaat cagtattgat ctgctggcaa gaacagacta
     6241 ctgtatataa aaacagtata acttcaggca gattattatg ttgtttatca agcctgcgga
     6301 tctccgcgaa attgtgactt ttccgctatt tagcgatctt gttcagtgtg gctttccttc
     6361 accggcagca gattacgttg aacagcgcat cgatctgaat caactgttga tccagcatcc
     6421 cagcgcgact tacttcgtca aagcaagtgg tgattctatg attgatggtg gaattagtga
     6481 cggtgattta ctgattgtcg atagcgctat taccgccagc catggtgata ttgtcatcgc
     6541 tgctgttgac ggcgagttta cggtgaaaaa attgcaacta cgcccgacag tgcagcttat
     6601 tcccatgaac agtgcgtact cgcccattac catcagtagc gaagatacgc tggatgtctt
     6661 tggtgtggtg atccacgtcg ttaaggcgat gcgctgatgt ttgccctctg tgatgtaaac
     6721 gcgttttatg ccagctgtga gacggtgttt cgccctgatt tatggggtaa accggtggtt
     6781 gtgctatcga ataatgacgg ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggc
     6841 gttaaaatgg gcgatccctg gttcaaacaa aaagatctgt ttcgtcgctg tggcgtggtt
     6901 tgctttagca gcaattatga gctttacgca gacatgagta atcgggtgat gtcgacgctg
     6961 gaagaactat cgccccgcgt cgagatttac agtattgatg aggcattctg cgatctgacc
     7021 ggtgtgcata attgtcgcga tctgactgat tttggcagag aaattcgcgc aacggtgcta
     7081 caacgtaccc atcttactgt tggtgtgggg atcgcccaga ccaaaacgct ggctaagctt
     7141 gccaatcatg cggcaaaaaa atggcagcgg cagacgggcg gggtggtgga tttatcaaat
     7201 ctggaacgcc agcgtaaatt aatgtctgct cttcccgtgg atgaagtctg ggggattgga
     7261 cggcggatca gcaaaaaact ggacgcgatg gggatcaaaa ccgttctcga tttggcggat
     7321 tcagatatcc ggtttatccg taaacatttt aatgtcgtgc tcgaaagaac ggtgcgtgaa
     7381 ctgcgcggtg aaccctgttt gcaactggaa gagtttgcac cgacgaagca ggaaattatc
     7441 tgttcccgct cgtttggtga acgcatcacg gattatccgt cgatgcggca ggccatttgt
     7501 agttacgctg cccgggcggc ggaaaaactt cgcagcgagc atcaatattg tcggtttatc
     7561 tccacgttta ttaagacgtc accatttgcg ttcaatgaac cttattacgg caatagcgca
     7621 tcggtaaaac tgctgacgcc cactcaggac agcagggata tcattaacgc cgctacgcga
     7681 tctctggatg ccatctggca agcgggccat cgctaccaaa aagcgggcgt gatgctgggg
     7741 gatttcttca gtcagggagt cgcgcagctc aatttattcg atgacaacgc accgcgcccc
     7801 gggagtgagc aattgatggc ggtaatggat acgctgaatg ctaaagaggg cagaggaaca
     7861 ctctattttg ccgggcaggg gatccagcaa caatggcaga tgaagcgtgc catgctttca
     7921 ccatgttata caacgcgaag ttctgattta ctgcgggtca aataaacata gcggcaggaa
     7981 aaagcgctcc cgcaggagcg ccgaatggat tagcgaccga acaggtcacg ttttttcgct
     8041 ttaaacggct gggaaatcac caccagcacc gcgacaatca ggtaagcgat aaaaataccg
     8101 agcagccact gcggcatttc cagacctaaa aattcccact gacgctcggc gcaatcgcca
     8161 gaggcgacaa acacttgcgg cacccattta tccagcggca gccattccgg gaaacgagcc
     8221 ataaaatcac aggtcgcaaa cggcgaagga tagagctgaa gcatggtgtg ctcgtaagtt
     8281 aactgcacac cgcggaacgc actatacaac cagataacca tcgctacata acgcagcgga
     8341 gttttcgggg cgatcgcgcc aatcagcgca gcacccagaa cgccgaataa tgcgcagcgt
     8401 tcataaatac agagcacgca aggtttcagt aacatcacat gctggaacca cagtgccgtc
     8461 agttccagtg ctagagcggt aaacgccatc aacagccacg cgccccggcc ttgtgaacat
     8521 tggttcaaaa atcgcaacat aatcatttcc ctgcaatatg catagagtgc gcagtttaaa
     8581 ccaattcatt cgctgcgcca ccaggaggca gcaaaaatag gacgcaattg gacaattatc
     8641 gggcgaaaag gcaaaccggg catttatatg cccggtaagt tgttatcaaa gcgttgctat
     8701 ccagcccatt tgcataaacc attcggttac aggggcaagc gtaaactcga cgcagagcaa
     8761 gccgacgagt gtcaggacga gggtgtaagg cagggccatc cacaccatgc ggccataaga
     8821 gaggcgaatc aatggcgcga gtgcagaggt cagcaggaac aggaacgcag cctgaccgtt
     8881 cggcgtagcg acggagggca gattggtacc ggtattaatg gcgaccgcca gcagctcgta
     8941 ttgcttcaac gtgatagcgc cactttccat tgccgctttc gcttcgttga tataaatcgt
     9001 ccccacgaag acgttatccg aaatggatga cagcagaccg ttgaaaatat agaacagcga
     9061 cagctgagca tgttccgatg cctgcaacac aaactgaata atcggcgaaa acagttgttg
     9121 gtcgataatc accgcgacta ccgagaaaaa caccgtcaac agtgcggtga atggcagaga
     9181 ctcggtgaag gctttaccga tagcatgttc atcggtgaca ccggtcaatg atgtcgccag
     9241 aataatgacc gacaaaccaa tcaagccaac ttccgccaga tgcaacgcca gcgcagtcac
     9301 cagccagacg ccaataatcg cctggacaat cagacggatt ttatcctgac gggtacgctg
     9361 gtggcggctt tgatcgtcaa actgttgcag tacttcgcgg actttctccg gcagcgtttc
     9421 accatagcca aaccaacgca gcttctctac cagcaggcag gttaacaggc cacaaatcag
     9481 aaccggaacg gtcactggcg acatgcgcag gaagaaatcg ccaaaatgcc agccagccgc
     9541 tttagcgatg atcaggttct gtggttcgcc caccatggtc atcacgccgc ctaatgcggt
     9601 gccgacaccg gcatgcatca tcaggctacg cagaaagcca cggaactgtt ccagaaccac
     9661 tttgtaatgc ttgtcgatat gactatcgtc ttgcaggtcg gtatcttcgg tacgggaaga
     9721 ggctacgcgg tgataaatac cataaaaacc gactgcaacg ctgatcacca ccgccacgac
     9781 ggttaaggca tcgaggaacg cggagaggaa cgccgccgcc acgcaaaaag agagcgacag
     9841 cagcattttg gagcgaatgc ttaacagcaa acgggtaaat atgaacagca atagctgttt
     9901 cataaaatag atacccgcca ccataaacat cagtaacagc aagacttcaa gatttgccgc
     9961 cacctcttca cggacgtgtt ccgcgctggt catgccgatg aataccgctt cgatagccaa
    10021 cagaccaccg gggagcagcg ggtagcattt cagggccatc gccagagtga aaataaattc
    10081 cgctaccagc aaccagccag cgacgaaagg gctgatgagg aaaattaacg gatttacgat
    10141 taagaaaatg atgagggcga gtttgtacca gtcgggggac tggcccaaaa aattgcgcca
    10201 tagtgcgcgg ccccaggaga tctccatgat ggtttccctt accttagaaa taatcaatga
    10261 tgtttttatg tttaaacgca aagcttaacg gtcagacagg agtgaggcaa gtcttgatag
    10321 tcaaggggaa agagatgcag aaaatgaagc cttgatccct tttttcttct ttttgtcttc
    10381 tatcagcata gttagccctc tggtatgatg agtccaactt tgttttgctg tgttatggaa
    10441 atctcactat ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag tacattattg
    10501 aaagtatctg gaataaccgc ttccctcccg ggactatttt gcccgcagaa cgtgaacttt
    10561 cagaattaat tggcgtaacg cgtactacgt tacgtgaagt gttacaacgt ctggcacgag
    10621 atggctggtt gaccattcaa cacggcaagc cgacgaaggt gaataatttc tgggaaacct
    10681 ctggtttaaa tatccttgaa acactggcgc gactggacca cgaaagtgtg ccgcagctta
    10741 ttgataattt gctgtcggtg cgtaccaata tttcaactat ttttattcgc accgcgtttc
    10801 gtcaacatcc cgataaagcg caggaagtgc tggcaaccgc taatgaagtg gccgatcacg
    10861 ccgacgcctt tgccgagctg gattacaaca ttttccgcgg cctggcgttt gcttccggca
    10921 acccgattta cggtctgatc cttaacggga tgaaagggct gtatacgcgc attggtcgtc
    10981 actatttcgc caatccggaa gcgcgcagtc tggcgctggg cttctatcac aaactgtcgg
    11041 cgttgtgcag tgaaggcgca cacgatcagg tatacgaaac agtgcgtcgc tatgggcatg
    11101 agagtggcga gatttggcac cggatgcaga aaaatctgcc gggtgattta gccattcagg
    11161 gacgataatc ccttccgttt aaaaagcaaa cccctcaaac gaggggtttt tcgttgtttt
    11221 tacagatttc ccattctcgg cgggcaacgt tccagcaact cgacgctgcc gtcttcgttt
    11281 tgctgttcca gcatcacatc aaatccccac aggcgatgca catgcttcag cacttctttg
    11341 cgcccccgat ccagcggtgc gcgattatgt ggaatgtaac gcagcgtcag cgaacggtcg
    11401 ccgcgcaaat ccacgttcca gatctgaata ttcggctcca gattacttaa attatattga
    11461 gacgataacc ggttacggat ctcccgataa ccttcttcat tatgaatagc ggaaatctcc
    11521 agataattat gccgatcgtc atccagcacg gtgaagaagc ggaaatcacg catcactttc
    11581 ggtgacagga actggctgat aaaactctca tctttgaaat cacgcatcgc gaaatgcagc
    11641 gtttccagcc agtcggaacc ggcgatatcc gggaaccagt atttgtcttc ttccgttggc
    11701 gactgacaaa tccgtttaat atcctggaac atggcgaacc cgagggcata tgggttgatg
    11761 ccgctgtacc acgggctgtt atagggcggt tggaagacca cattggtgtg gctgtgcaaa
    11821 aactccagca taaaacgttc cgttactttc ccttcatcat acagatggtt aaggatggtg
    11881 tagtgccaga aggtcgccca gccttcgttc atcacctgag tctgtttttg cggataaaaa
    11941 tactggctca ccttacgcac aatacgcagg atttcacgct gccatgattc cagcagtggc
    12001 gcatttttct ccataaaata gagcaggttt tcttgtggtt cggacggata gcggcgcgct
    12061 tcagcaaccg ttttctcttc ctcgcgcttc ggcagggtac gccagagcat attgacctga
    12121 ctttgcagat actcttcgcg actcttctgc cgggctttct cttcttgcag cgagattttt
    12181 tgcgggcgtt tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga gtccagaagc
    12241 cgttctactt catcaacgcc ataacgttct tcgcactcgg taatatattt acgggcaaaa
    12301 atcagataat cgacaatcga actggcgtcg gtccagctac ggaataagta attgtttttg
    12361 aagaaagagt tatgcccata gcaggcatga gccatcacca gcgcttgcat ggtaatggtg
    12421 ttctcttcca tcaggtaagc gatacacggg ttagagttaa tgacgatttc ataggccagt
    12481 ccttgctgac cgtgcttata cagccgttca gtctcgataa actttttacc gaatgaccag
    12541 tgcggatagt taattggcat gccgacgctg gagtaggcat ccatcatctg ttctgaggtt
    12601 atcacttcaa tctggtgcgg gtaggtatcc agccggtaga gtttcgccac ccggtctatc
    12661 tctgccagat aaacatccag caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg
    12721 gtgtccttat tcatagaatc gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct
    12781 ctgtgtggag cacctcattt caagcataga acacctgtta aaaaccgcgt cgccggagaa
    12841 tttttttctt tgcgatttct tattatcaga gtgccactaa tccgcttctg aacggaattt
    12901 tatgctggat aaaaagggcg ttcagcagga gatactaaag acgccatatt gccgcagagt
    12961 cagggagatg tgagccagct caccataaaa aagccgtatg ttgaataata ttttcaactg
    13021 agttatcaag atgtaattag attattattc ttttactgta tctaccgtta tcggagtggc
    13081 tatgcgagtt gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa
    13141 tcaggcagga catgaggtca ccgtcattga tcgggagccg ggggccgccc tggaaaccag
    13201 tgccgcgaat gccgggcaaa tctcccccgg atatgctgca ccgtgggcgg caccaggtgt
    13261 gcctttaaaa gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga
    13321 cggtacgcag ttccagttga aatggatgtg gcaaatgtta cgtaactgcg acaccagcca
    13381 ctacatggaa aacaaagggc ggatggtacg tctggcggaa tacagccgtg attgcctgaa
    13441 agcattgcgc gccgaaacca atattcagta tgaagggcgt cagggtggga cgctgcaact
    13501 gttccgtacc gaacaacagt atgaaaacgc gacccgcgat atcgccgtgc tggaagatgc
    13561 cggcgtaccg tatcagctgc tggaatccag ccgcctggcg gaagtggagc ccgcgctggc
    13621 agaagtggcg cacaaactga cgggcggcct gcaactgccc aatgatgaaa ccggagactg
    13681 ccagctgttt acccagaatc tggcgcggat ggcggagcag gcgggggtta aattccgctt
    13741 taatacgccc gttgaccaac tgctttgcga cggcgagcaa atctacggcg tgaagtgtgg
    13801 tgatgaagtg attaaggccg atgcgtatgt gatggcgttt ggttcttact cgacggcgat
    13861 gctcaaaggc attgttgata ttccggtgta tccgctgaaa ggctactcgc tgaccattcc
    13921 aattgcgcag gaagatggtg cgccggtatc cactattctt gatgaaacct acaaaatcgc
    13981 cattacccgt ttcgataacc gcattcgtgt cggcggaatg gcggaaattg ttggttttaa
    14041 caccgagctg ttgcaaccgc gtcgtgaaac gctggagatg gtggttcgcg atctctatcc
    14101 acgcggcggt catgttgagc aggcgacttt ctggactggc ctgcgcccga tgacgccaga
    14161 cggcacgccg gttgtcgggc gtacacgctt taaaaatctg tggctgaata ccggtcacgg
    14221 cacgctcggc tggacgatgg cttgcggttc cggtcagttg ttaagcgatc tgctctcggg
    14281 gcgcacgcca gcgatcccgt atgaagatct aagcgtagcg cgctacagcc gtggatttac
    14341 gccatcacgc ccgggccatt tacatggcgc acacagctaa ggaaacgaga tgacccgtcc
    14401 gatacaggcc agcctcgatc tgcaggcatt aaaacagaat ctgtccattg tccgccaggc
    14461 tgcgccgtac gcgcgcgtct ggtcggtggt aaaagcaaac gcttacgggc acggtattga
    14521 gcgtatctgg agcgcgctcg gggccaccga tggctttgca ttgcttaatc tggaagaggc
    14581 aataacgtta cgtgagcgcg gctggaaagg accgatcctg atgctggaag gatttttcca
    14641 tgctcaggat ctggagattt atgaccagca ccgcctgacc acctgcgtac acagcaactg
    14701 gcagctcaaa gcactgcaaa atgcgcggct aaaagcaccg ttggatattt atcttaaagt
    14761 gaacagtggg atgaatcggt tgggcttcca gcccgatcgc gtgcttaccg tctggcagca
    14821 gttgcgggcg atggcgaatg ttggcgaaat gaccctgatg tcgcattttg ccgaggcgga
    14881 acatcctgat ggaatttcca gcgcgatggc gcgtattgag caggcggcgg aagggctgga
    14941 gtgtcggcgt tcgttgtcca attcggcggc gactctgtgg cacccggaag cgcattttga
    15001 ctgggttcgg cctggcatta ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat
    15061 cgccaatacc ggattacgtc cggtgatgac gctaagcagt gagattattg gtgtccagac
    15121 gctaaaagcg ggcgagcgtg tgggctacgg cggtcgctat actgcgcgcg atgaacagcg
    15181 aatcggcatt gtcgccgcag gatacgccga cggttatccg cgccatgcgc ctaccggtac
    15241 tcctgtttta gtggacggcg tgcgcaccat gacggtgggg accgtctcga tggatatgct
    15301 ggcggttgat ttaacgcctt gcccgcaggc ggggattggt acgccggttg agctgtgggg
    15361 caaggagatc aaaattgatg atgtcgccgc cgctgccgga acggtgggct atgagttgat
    15421 gtgcgcgctg gcgttacgtg tcccggttgt gacagtgtaa cttgttatcg ctggatgcga
    15481 ccagcgtcgt atccagcgtt acaaaacaca ttaaacttaa gattcggctt cttcctccgc
    15541 tacccgaaca ccaatcttca acacttcatt atcctctttc tcggccaccg tccagatcat
    15601 tccggcaaat tctacctgat caccgacaac cggtgccgcg cctaacaact gctggacaat
    15661 ttcacccagc gtttgctgct tatcacgata ttctcgcccg tcttccaggc catatatcag
    15721 cgccacatca gcatatttgg cactggcttc gagaatgaag tcaccaaaga agcgttgatc
    15781 tagcgcgacc ggtggcgact ggctgaacag tttaccgagc gccgggagat cgcgttcccg
    15841 accaattaca cacaacacat cgccttcgcg cagtcgggtg ctgccggtag gatgaagcaa
    15901 ctggttatca cgaaacagtg ccgcaatacg cgtctctttt ggcatatgca gatcgcgcag
    15961 tgccgcgccc acgcaccatt tatcggcact caactgataa acaaactgct cccacggatt
    16021 ttccggatga atatccagac caacgcgtga caccggacgt cccactggcg gaacgaccac
    16081 tttggctttt ttcgccgccc acgagagtga tgtcccctgc aagagtagtg aaaccagaac
    16141 cacaaagaag gcgacattaa agaacagtcg cgcattctcc agccccgcca tcatcgggaa
    16201 cacggccagg atgattggca ccgcgccgcg taatcccacc cagctgataa acacgcgctc
    16261 acgcagattg aagccacgga acggtagcaa tccggcaaat accgaaagag gacgggcgaa
    16321 gaaaatcatc catgcggaca aaatgagcgc cggaatggca ataggcagca gatcgcttgg
    16381 gttaaccaac agccccagca ccaggaacat ggcgatttgc gccagccagg cgaggccgtc
    16441 gaaattttgc agaatgccgt agcggttgcg aatcggccga ttacccagca gaaaaccgca
    16501 cagataaaca gccagaatac cgctgccttc cagcgccgtg gttaaagcaa aaattaaaat
    16561 accgccgctt aatgccagca atggatataa accggcgggc agggcgatgc gattaatcat
    16621 ttgcagcagt aaataaccac cgccaagacc gatgacaatc ccgaggccaa attgctgcaa
    16681 aatatcgacc acgaacatcc agctgacact gctttcatgc tgctggatca tcgcaatcag
    16741 ggtaatcgtc agaaagaccg ccattggatc attactgccg gattcaattt ccagcgtcga
    16801 gccaacacgt tcgttaagcc ccttaccacc cagcagagaa aagaccgctg cagcatcggt
    16861 ggagccgacg atagcgccga ttaataatcc ttcaatcaaa tcaagattaa acagccacgc
    16921 cgccatcatg ccggttaaac cagaggtgat aagcacgccc agcgtcgcca gcgacagtgc
    16981 cggtcctaac gccacacgaa aggaactggc ctgagtgcgc atcccgccgt cgagcaaaat
    17041 aatcgccagc gccagattac ttaccatgta ggcgaaggga taattatcaa acgggatacc
    17101 gccgacgcca tcaacccccg ccagcatgcc gatcgccaga aaaataacca gaatgggaat
    17161 gccaagacgg gaagaaaatg aactaagtaa aatactgctg gtgacaagga tggatcctaa
    17221 gatgaaaagg ctaattattg ttgtggcatc caacggtcgg ttactcctga ttacgctgtc
    17281 tcttatataa accctaccat attagcggca gagacagcgt tttacttagc cctgaagcgt
    17341 gattttttta cattttaaga acaggatgac cgctaatcgt caactgggtg ccttcctggg
    17401 tgttattcag aatggcatgt gcgcccaacg gcagtgtaac cgtgcgttgt tcatgaccaa
    17461 aatcgaggcc ggtaatgagt ggaatcgaca ggcgggaacg caaaaacgcg tacactgact
    17521 ccaggttgta acctgcgtca taatcattag gcgtgctgcc gctaaagcta ccgagaataa
    17581 tcgccttctg acgtggcaaa attccagcat gatagagctg caacagcata cgttcgacgc
    17641 ggaaagggtg ctcgttaata tcttccagca ccagaatacc gttctcaatt tttggcatcc
    17701 acggtgtacc aatcagtgaa atcagcatcg cgagattgcc tccccacagc gtgccttcgg
    17761 tctgacatgt cgggccttcc ccttgccatt caatggtgaa ggtttcattg cgtaacgcca
    17821 gccagaaatg gtgctcggta aaggcgttca gttcatccgc gccaaagttt gctaccagca
    17881 tggggccgct aaaggtgatg acattgccat gcgccagaag accgcactga atggcggtaa
    17941 aatcgctatg tccgcaaatg agcaacgggt catgttgttg gcgagccacc agtgcctgcc
    18001 agtcaatatc tgccagtaaa cgactggcac cgtaaccgcc gcgtacagcc agcacgatgg
    18061 tgttgggggt agtcagtctg gcaagagaat tgagatcttc cagacgctcc gtttccgtac
    18121 ccgcaaaacg ctcacaacga cgggcaataa cctcgacgtt attgacctga tgccccgcgt
    18181 cggtaaggcg ctggatacca cgcagcgcgg cgtgctgttt aatgcagtaa cccgatgggg
    18241 caattaagtg aaacagagac atggcaattc cttgctgaca acagaaacga aatgtatatc
    18301 atgccgctta ggtgtgccgt tgtcacctca acggcgattc caggctataa ggatagaaga
    18361 agtgaaattg agatggtttg cctttttgat tgtgttatta gcgggttgtt catcgaagca
    18421 tgactatacg aacccgccgt ggaacgcgaa agttccggtg caacgtgcga tgcagtggat
    18481 gccaataagc cagaaagccg gtgcagcctg gggcgtcgat ccacaattga tcacggcgat
    18541 tatcgctatc gaatcgggtg gtaatccgaa cgcggtgagt aaatcgaatg ccattggttt
    18601 gatgcagtta aaagcttcaa cctccggacg tgatgtttat cgccgtatgg gctggagtgg
    18661 tgagccgacg accagcgagc tgaaaaatcc ggagcgtaat atttcaatgg gggcggctta
    18721 cctgaatatt ctggaaaccg gcccgctggc aggcattgaa gatccgaagg tactgcaata
    18781 tgcgctggtg gtgtcatatg ctaacggggc aggtgcgctg ctacggactt tctcgtcaga
    18841 tcggaaaaag gcgatcagca aaatcaacga tctggatgct gacgagttcc tcgatcacgt
    18901 agcgcgaaat catcctgcgc cgcaggctcc gcgctatatc tacaaacttg agcaggcact
    18961 ggacgcgatg taaatcagtc gcgcactttg tccgctttct cccgggcttc tcgctcgaga
    19021 gagaaaataa tccgctgtaa ttgccgctcc accgtcgggc tgacgttaag aaaacggaag
    19081 ctcagacggg gagtggtgat ggtttcattc ttgccatcaa tcactttgcg ctcgctgatg
    19141 gagattaact gggcgtcaaa gtgaaaaaca ccccattgcc ccatgttgac ttcaatctga
    19201 gcgaagcgca tgccttcgtg caatccggca ggctttgctg tttccagtaa tgcgcccatg
    19261 ccgcctaacg acaaatcata caggcggaaa cgtaacgtac tgttatccgc cagtttggtc
    19321 tggcaaaaat atggcggatg gagtggggcg gagatgcgga aatatcggcg tcgttgtaca
    19381 aaccacaagg tgggaggcgg tacggtaata aatgccggaa gctgcaagta ttcactctgc
    19441 tgtagttgtt caacagtaaa ctcgactttc gcaccctggg tttcggcggt aatggtaatg
    19501 tgctgcgcct ttaacacagc gttgttatct tcggcttgac tgccgaaatc cagcaccagt
    19561 ttatccgggg ttattgccag tattttgctg atcagctgcc cgccattcca actgatacgc
    19621 aaaggaattg cggctttgtg caaatcgcgt aacacgccca ggacggctaa cggattttgt
    19681 ttcaggaact gctcatggta atgactcacg cggaaaaact cctgactgac aaactgtctt
    19741 aggtttatcg gtcacagtta acaaaactta atacaaatgc gtgaatattt tttacatgtc
    19801 gttcttaaat cagccgacat acgcccagca tcgatcccgc ccttgcctat acttagagcg
    19861 ttgacgtaag catttcttgc gtccgattca tcgaaacgag ggcctgaaca tgggaattat
    19921 tgcctggatt atttttggcc tgatagccgg cattatcgcc aagctaatca tgccggggcg
    19981 tgatggtggt ggatttttcc tgacctgtat tcttgggata gtcggtgcgg tggtcggcgg
    20041 ctggctggcg accatgtttg gcattggcgg ctccatcagc ggttttaatt tgcacagctt
    20101 cctggtggcg gtagtgggcg ctatcctggt gctgggcgta ttccgcctcc tgcgaagaga
    20161 ataagatttt cataaggcgg atagcgatac agatgccgct atccgctttc acatcagaac
    20221 gtatactcga tacctgccca gaaattgcga ccttcttcaa taaacccttc actgtaggca
    20281 tatttggtat caaacaggtt attgaccgac gcattgacgc tgaagccatg acctaaggtg
    20341 taatcggctc gaatgtgggt caccgcaaaa ccggcggctt tttgtgaacc gtcactgttg
    20401 ctgtagctgg aggaacgcgc ctcttccgac agcgttacgc ttaacggctc ccacggtttg
    20461 agagtcatcc atgcggtcat tgtctgcgtt ggcagatcgg ttatcttgcc gatgtctttg
    20521 cgtttggcat cagcgtggat cagggcgtag ctcaatccta catccagtat attgctgatt
    20581 ttccccttaa taccggcatc cagaccgctg taatccaccg tgccgctgtt ctgattttga
    20641 atggtatcgg catcgatatt gtgcgagagg atggcatcac tcacccggtt atagtaaacg
    20701 ctgacctcaa acccccagtc gtgcgtgaag gcaccattcc aggttaaatc caccccgcgc
    20761 gcgcgttccg gtttgagctg cgggttaaca atcgctatct ggttatacgc aggtttggac
    20821 gtggtatagc gttctttcag cgtcggaaag cgtgtgcggt catagtacga aagcgccagc
    20881 gtgtcttcat tggcaaagtg gtatttcccc atcacctgcc agttaaaagc tgactgattg
    20941 ttgtcgtcat aatgggtgat gcttccatct ttctcatgtt ttttcgcttc tacgctatcg
    21001 cgccagtcat agctgattcc agccacgaca tcgacattat cggcagcagc ccattgatat
    21061 tcactggcga gcgaccaggt acgatcttca tagcgatcgt aagcggcgtg cggcgcacct
    21121 ttttcccggt gtacgtcatc tttccagtta acggcaaacg acagcagatc gttttcacgc
    21181 acatcggctg ccagttgtaa cccggcaccg tcgctgtaat cggaataatg gctgtagctg
    21241 ccttttttat ttttcaaatc agccagcgag ttgtacatca tcagcgtatt ttcaaaggta
    21301 tcgcgataca gccgactttt cagggtaaaa cgatcgttta gttgggtcgt tccctgataa
    21361 taaaaacttt ctttgtcata ctctggccac tgccagtagc gtgatttttg accactattt
    21421 ccactgtatg gcggattatc tttttcacca tcctgcttaa tgtaagtcaa tgtgtattca
    21481 tcgttttcgc gtggtgtaaa accgagcttc acaatgccgc gtttatcatc agccgatgaa
    21541 ttaatcatct tgccgtgttt gcctgcaata tcattattta caccatgcgg caggccgaga
    21601 aaatcctgct ttagctggct accgctgact tgcaaatacc ccagatcgct gctggcggca
    21661 aatgaagcat gcatatcgta ggcattgtcc tggctacggc tccatccctg gcgatatccc
    21721 agatttgctt ccagaggttt tgttggcttc tgggtggtga tattaatggc tccgcccatc
    21781 tgattaggcc cctgaagcag cgacgaatac cctttggaaa cttcaactgc ccccaaattg
    21841 ttggtcagaa tccgcgccag atcgaggttg ccgtcatagg gaacataaat gggcacaccg
    21901 tcgaaataga ctggcacctg acgactatca aagccacgaa ctttgacctg ttcttcgttg
    21961 cggctacctg acttttgcag caccacgccg gggacgacac ttaatgcctg agcgacattc
    22021 tgtttatcca gggccttcat ggtggggtga tcgagaacgg tggtcgtcgt cgatgatacc
    22081 ggactggacc atacggtcag cgtgtcactc tcctgagccg ctaaggcctg ctgggtaaag
    22141 gcaagtgata atgctgtgca gagataacgt tttttgagtc tcatagaata aattccgttt
    22201 aacaaagaat taaatagagg ctgcgggttg cagactgact ttaatgtcag ctggtgtcgc
    22261 gtaataaggc gcggaggtaa taaaaaggcg aatgccgcag tcgaggtaat ttttgagtgt
    22321 tgtcagatta attccgccgg tcagagccag cgtgcagtga ggagccagcg acggtgctat
    22381 ttgcgcaatt tcagttgcct gttgcggatt aaatttgtcg agctgaagta cgtctggttg
    22441 cgcgcgtaac gcggcgattg cctctttcgg cgtgtcggct tcaacaacta ttttcttctc
    22501 gggtgcgtgg cggcgtaact gattgattgc gcctgaccag tcctgattgt catgaagaaa
    22561 atgacggtgg ttggcaaaca ataatatggt ttccgcacat ccggcacgat gaatcagtcc
    22621 tccggcagcc agaattgcct gcgaggccag taagcgggtg cccggaattg cttttcgggt
    22681 gcaggcgata ttgccatcag ggtaacgttc acgaagtaac gccagcattt gagcgagata
    22741 atcagaaaca ccgcaactcc actccagcac attctggact gccttccatc cctgatgaag
    22801 tgctgcggca ttaccctgcg cacggattag ccgctgaccc gcgttcgctt gtgaaccgtc
    22861 gctgactgcg tcatcaatag ttaatcccag cgtagttaac atcttacacg cgacagaaat
    22921 tccgctgacg cagccgccct gacggtgaaa aaactctata tagccatgct ggtgtccaat
    22981 atttaatgct cgcgtggtca ggtcaccgcc ctggatatct tccagaagta acgcatcgat
    23041 ttgtgcctga gaaaggaaaa tcatcgcagt gcctcttgcg gtacgctatc ccaccagatg
    23101 agcgcccagt cgcggctggc aggggcgagg gcacgagcat cttgttgttg ataccagcga
    23161 tataaccgct cacgctcatc gtcgttaagc gcgcccagac tccagctgac gttctgctca
    23221 aaacgttccc aggtgctgtt atcctgttgg caattttgtc cgcggataaa atcaacgtga
    23281 gcataaattc ccatctgata caggacgttg agcgcaaaga tatagttggg taattcgacc
    23341 acttcccgac cagccgcccg ctgaatggct ggagagacga acgaggtgct gaccagatga
    23401 gtcgtataga cgcgaagccg tgcctgatta ttcagtttgc tcattgcctg gcgcatatca
    23461 gccaccagag tggagcggga agccacggca atatcgcagc gtggcaggtc actccagtcc
    23521 tcttcccagg cgcgctggat ccagtggaca ttgtctgcct ttagcgcggc ggcgcgacgg
    23581 gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat aaatcgtcgt gagtttgtcc
    23641 gccagcgcca gcgatacggt acccggtccg cagcccatat cgaataaggt ttgcgcgcct
    23701 tgcaggtcaa tttttgaggt cagttgttgc aggtagctgt ccgtcggcga ggcgcaattt
    23761 tccgccattt tttccgcccg ttgatcccag tgatccggcg ttttttctgt gcgatgggcc
    23821 agcctgagtt gctgaaggta aaggtctgca aaatcaatat cgtcaatgag catgattttt
    23881 ccttagtgct taattgcaga gaggtgatgg tgaatctggt cggcagaaac ccggtacagg
    23941 gcagccagtt ttttcgttgt taactgttcg gtcggtaagc cttgtgttac tcgtccatct
    24001 ggctctacct ggataatgct gtcggcaatg gcgtttgcat ggagcggatg atgagtcgac
    24061 attaacattg tcatgccgtt atttttgagc tgcgccagcg tgtccagaag ttggatctga
    24121 tgaccgaaat cgaggctaga ggcaggctca tccagtaata acaagcgcgg ttgctggacg
    24181 agtgcacggg cgattaaagc cagctgacgt tcaccgccgc taagcgtatt ccagcgtcgt
    24241 gtcgccagat gcaaaatgcc cagcttttcc agttgctccg ttgctttcag gcgttcttgc
    24301 ttaccgggaa cggaaaaagc gcctatggtg ggggcctggc ccatcaacac catatccagc
    24361 acactaaaag caaaaatgcc gtcgtgcgcc tgcggcaccc aggcaatagc ctgcgcgcgc
    24421 tggcgatggc tgagtgtctg cacagggagg ctatccagta agatctcgcc ttttaataac
    24481 ggtataacgc caagaatgga gcgcatgagt gtggttttgc cgcaaccatt ggctcctaac
    24541 agacaacaaa ttgttccttg cgacagggtg aaggacacgt cgcgtagcac cggatggcgg
    24601 gagtaaccca gagacgcatg acggacggca agcaaagtca ctggcgacct ccgcgtaaca
    24661 acaaaaacag gaaaaacggt gcgccaacaa aagcggtcag aatgccaagt gggatttctg
    24721 ttgttccaat gctgcgtgcc agcgtatcgg taagcaggag cagaatcgcg ccagtacaca
    24781 ttgccatggg caacagctgc tgatggttat gaccggtcaa caagcgggca atgtgcggaa
    24841 cgaccaggcc gacccaacca attatgcccg ctattgctac cgtgctggcg gtgatgagtg
    24901 tggcacagac aatcaatcca aagcgcagac gtgtcacgtt cagtcccagc gaacgggctt
    24961 cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag aaaaaacagg ggcaaggagc
    25021 ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa ggtcacggtg gacagccccc
    25081 ccagcaacca gaaggtaatt gacggtaact gggtataagg atcggccagc gtttttatta
    25141 gcgatatccc cgctccgcac aacgttccta acgcaatgcc taccagcacc agggttaaga
    25201 tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac gcccgcgacc accatcagcc
    25261 cgccgcaaaa tgcgtacagc tggatgtaaa caatggataa tccccacagg attgccgcgc
    25321 aggcaccaag tccggcaccg gcggagacgc ctaaaatatc cggtgacact aagggattac
    25381 ggaacattcc ctgataggtg gttccggccc ctgccagcgc cgcgcccaga aacagcgcag
    25441 caagaatacg cggcacgcgg atttgccaga agacaatttg ttcctgcaca gtaacatttt
    25501 cttgacgaag aatgagcgcc agcagttgct gcatatcgag atgataagcc ccacttacgg
    25561 cggcaaaagt gatactgata gctgccagca gcaaaataca tccgtttacg gttctcatta
    25621 gctcgccacc aatttttgat agtcggcgtc acttaatggg caatgccaga acagttgggc
    25681 gtaatgctgc atgtcacttt taaactggcg attgatggcg ggatccagcc aggcgtgaag
    25741 tctgcgtaat cccagaagac ggttgattcc cggcggggca tccagccagc caaagggcag
    25801 gccacttaaa aataagatgc gttgttccgc aaccgctttc acgccctgcc agagaggatc
    25861 acgacgaata aaatctgcag taacggcctc ctgaaccaga ataatatccg gctgccagcg
    25921 caggagattt tccatggaaa cctgtgtcag accgtggcga tcggctattt gcgcgacgtt
    25981 gtgcaaacct aataattccg ccgcctcggt atgcaacgaa ccctgtaagc ccgtttccag
    26041 ccctcgagga ccgcgcgcag cataaaagct gagattagcg gcgggtgagg tggcgaatgc
    26101 ctgagcttca ccaacgaagc ggctggcgag attggcttgt tctgcggcgc ggtgctcttc
    26161 gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat tgttccagtt tcccgttaag
    26221 caataaccag ggtatttgtg tctgttcgct aacctgccgt gcctgggaga tccaggtttc
    26281 atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac gccataagcc cttccagcga
    26341 gagtgtactg gcgcgcccgg cgagtcgtcc cagcctgggg aactggcgaa tgtgctctgg
    26401 caatggaatt aatgcctgac gggcaaaatc aaaagaggaa aaaccgacca ttttttctgg
    26461 cgcgacagcc agcaatagta aatcggcggg cgcgcctgcg ctgacgatgc gtgtaatgtt
    26521 ttgggcttcg ggaagcgtca gtgaggccca gaatttgact gattgtgcaa aggacggcag
    26581 atgatgcagg aacaatgtcg acgcaagaat ctgtgtaaac ctccgtctgg ttagtgacat
    26641 aagtaatatc ctgataatta aaaccgctat atacttacac atatagcgaa aaagatcatg
    26701 tttgatgtca aaatttgtct aaagagacgg aatgatttgc ttgttgctta aataacactt
    26761 tgttccacaa gaaaattgtc agggagaatg gggagtgggg gaaaatataa atagaggtgt
    26821 aatgattaac aagttgataa aataaaatta aaatcttctc taataaaaga aaataaaggc
    26881 atagaccgta gaaagagtgg tatttggtat ttcgtggtga agaaagagca atgccaattt
    26941 tatgcacgta atggcattgc tcgatataca gtaaagttaa ggcgtaggtt ccgcctcatt
    27001 ttgttttacc ggctgtgttg ttgattcact gagtggacgc gtcgccggaa cattgtcgca
    27061 cggttgctct ttcgggcaga tcaaatccag cattttcagc gtcacgccat tggtccagcc
    27121 aaagccatcc tgtaatggat attcgccacc gccgccccct gttccggtgg tgctgacatc
    27181 atatttttcc accagctttt tctcccggtc ataggtgtgc tgaacattgg tcaggaagtg
    27241 ccagctaatg tccatcgcca cctctttttg cccgtagttt tgtaatcctt ctgtcgcgac
    27301 ccactgcaac ggtgcccagc cgtttggcgc atcccattgt tgcccacttt tcaccgacgt
    27361 ggtgttcagg ccgcctggtt gcagcagatg tgttttcgtc gccgtcgcca ttttgctggc
    27421 gcgatctttc gctgccgcat tgacgtacag cgggaacagg gcggccgcgg ttaactgatt
    27481 gcgcacttta tgacttttca ggtcgtaatc ggcataccag ccttgttgat cgttccacag
    27541 gtatttttcg atcccttttt gacgggcatt cgccagcgtt tcgtactggt ttgccatcgc
    27601 gttatctccg gcagctttgc tggcgcgggc gaggattttt tccattttaa acatcaggct
    27661 gttcagatcg accggtacga tgctggtggt gcgtaaggta tttaactgct gcggattgtc
    27721 catccagcgc gagctgaaat cccagccaga cgcagcggca gagcgcaggt cgcggtaaat
    27781 ttcagtggca ggtcgattcg gattgctttt ggcggtggca atatcttcca cccatgactc
    27841 tggtcgtggc gtatcgcgat cgtcccagta gcggttgaga agggtaccat cctgaagttt
    27901 gacaacgcgt ttttcctgtt gtccggcttg caggttttca acaccgtcca tccagtaagc
    27961 atattctttt tgcatttgcg gcaggtattg cttcaacgcg gcatcgcctt catgctgcgc
    28021 cagtaactct accatcaggg caaagaatgg cggttgtgag cggcttaaat agtaactgcg
    28081 gttgccgttg ggaatatgcc cgtaagtgtc tatttcatga gcaaaattgg ccaccatatc
    28141 cgcgacttta tcccagtgac cactttcggc aagccctaac atggtgaagt aactgtccca
    28201 gtaatatacc tcgcgaaaac gtccgcccgg cacaacataa ggttccggca gcggtaacag
    28261 agaatcccat ttttcggtat tttcggtaga acgcgttaat accggccaaa gtccgtcaat
    28321 atgttcgcgt agtgattgcc cctctggcgg aacatatttc tcgccctctt tcggcagggt
    28381 gaaattgacg ttaacgaaat ggcgtagatc aaatccgctc tggttttgct gcatccgata
    28441 atcagcaagg atcatcagcg gatcgctgtt cggcacggca tcggcaaagg ttttttggtc
    28501 cggaaaaagt ttggcgtttt gcacatcatt aaacagcggc cctaataaaa tatcaggcgg
    28561 ctgtggcgtt accggtgttt cttctgcctg caccgatagc gcagcgaaac acaaaaagat
    28621 gcaggctgga attaacgcca tttttagcgg gcgagaagct gcgggggatt tcatcaatca
    28681 ttctcctttg gcgaaaccga ataaaacggt attcaagtat cagaaaacct tagttcagga
    28741 tcgcactagc tgcatgatcg aaatctcatt ctgcgaacgg tcaggcattt tagaaaaggg
    28801 aattaaaggg ataaataacg tcgaaataga gtgatcaaat aacgtctgtt tgttagccag
    28861 ccagcggcgt tgggttcgcg attagcgcag aaaaaatcat catgatttgg taaaaaatat
    28921 aacaggtagt taaaaatcat ccgtgcagag taaatagccg gatggcatca gaacgatgcc
    28981 atccgaacgg tggtttaacc ctgacggctg aaacgttgcg ttttaacgtc cagcgttagc
    29041 gtttcttctg gttgtatcgc atacagtttc tcaccctgct ggcaaatcca gccaatcccc
    29101 agttcacggg cgatcagggc gctgtgggat agcggacttc cggcgctaag gcagatacct
    29161 tttacaaccg ccggatccag ttgcagtatt gtggagggat agatgttctc cgccagtaga
    29221 atagttggcg agttaaactg cgggagttct tctttcgttt gggtcaggtg gaccagggtg
    29281 cgatgcagaa gatcgtccac atcaatatag cgagcctgta gatattcatc atccagttgc
    29341 tggtattgct ggctaagttc tttaagaact tgctgccagg catattctgc cgtgcaatgt
    29401 tcatgctgaa ggagttcgct tgccgccgcc agcagttccg gatcatctaa cagtgtatgg
    29461 tgaccagaaa agattgcggc aatatcgtca agcccgctgg cttctgcttt cgctgttaac
    29521 gtcatcagat ctaataacgt gaagtcaatg gcctggcgta atcgttcttg ttcttcatcc
    29581 acggtcaggg ttgattttgc ctgtaccgta cataaaaccg gttgataata aaaggcttta
    29641 cccgaaacag gcggaacggg acgcagaata ggtggagcaa cttcctccgt ttcaccaaag
    29701 ttatcttcag ccagctgacg gaaagcgatc agtgcctctt cagcttctgg ccctttcgca
    29761 atcaggcgca gcgtatcgtt atagcgaact tgtagtaacg caatctggtt aatactctcc
    29821 ggtgtgacgc atttgccgtt tttctccagc aacatatcgg cattaaatgt cgataaggta
    29881 taaaccagcc gggaggccgg acgtacatgc aggccgttac ggttttttat gaccaccgcc
    29941 agagaacggg cttcttcatc gtacgaagga catgtgtcag agatttcagt gttggaggag
    30001 ggtaaaccca gttgttcacg tttggcttcc agcgcatgca tggcgtcaaa gatcactttg
    30061 tcgatctccg cccccgaggc cgcgctaacc gttgctgcca gtgtaccttc gaccaacggc
    30121 gcagcacaca aacgtacttt tgcggcgatc tcgggagcca gcaattccag cgcagtttca
    30181 gcactcaata atgcgcttcc tatatccatc atgaccagca catggtcggt atcggcaaca
    30241 gattcgatgg cctccatcac tttgacggca tcggtaccaa tgggattttg tggatcgtca
    30301 attcccgcgg caatggcgat tttacaatta tcactcatta acatctgacg ggctaattca
    30361 ccgacgcctt ctcccagtcg gctgctatgt gaaactatga ccaggtttac catcaccaat
    30421 tccttactct tttgcggcta acgccaacat ttgcatcata aacatcaccg aggtcgcacc
    30481 ggggtcctgg tgaccaatac tgcgttcacc gagataactg gcacggcctt tgcgagcttg
    30541 catcgtaatc gtactttgtg cagcggattc ggcgacgcta ctggcagctt cgagcgccgc
    30601 cggaatagag agattttgct cgctggactg acgtaacgat tccaccaccg gcacccacac
    30661 atcacacatg gttttatcgc caggttcggc tttcccgcga ctgattacgc cgtccgcgcc
    30721 atcgcggaac atctgataaa gctcttccag cgtcaggctt tgccgtgcct gggtcgcctg
    30781 tgcggcgcgg ataaagaagg taccgaaaag cggaccactg gcaccaccga cgctggaaag
    30841 tagcgtcata ccggtattct tgagaatgaa accgatatct ttatctgcga tagcagggag
    30901 tttttccacc actttgctaa agcctcggtt catatttagc ccgtggtcag cgtcgccaat
    30961 ttcgcgatcc agtccggtaa gatactcgct ctcggtgctg aaaatatcgc cacaacgagt
    31021 gagccagtta acaatttgag ttctgctcag tgacattgct atctccttat ttaccccagt
    31081 taagggccgg ggtgtggacc ggggcgtccc agagtgccag cgtttcgtca tcaactttca
    31141 gtaaggtgat tgagaaaccg gtcatatcca gtgaggtgca gtacgcgcca attaaattac
    31201 gctcgatagt caatcccgct tgctggcaac gtgtggtcag gcggttatag acgccgtaca
    31261 gctcagaaag cggagttgcg ccaagattgt taaccagcgc aatcacccga tcgccagact
    31321 ggagcggttg tttagtttgt gattcttcct gccaactgcc ttgttgataa tcccagaaac
    31381 gcaaagtgcg atggtatgag ccattttcca gcagggtgtc gaacatttca tcgacggttt
    31441 gatcaaggga agagaaggga cggcggtcaa tacctggctc accatgaatg ccgacgccaa
    31501 actccatctc attgtccgcc agggtaaaag aaggtttgcc cgcggcagga acggtacagg
    31561 caccgagagc gatacctatt gagtggcctt gattattcaa cttacgcccc agttccgcac
    31621 aggcgtccag tgagtcgcca cgctcggccg ctgcgcctac gagtttttca attaatacgg
    31681 tgttggcaac gccgcgtcgc ccggcggtat aaagactgtc ttttaccgca acgtcgtcat
    31741 caatgaccac agtggtcact tttacgccgc tatcgtgcag taactcggtc gctgtttcaa
    31801 agttaagaat atcgccggtg taatttttga taatcaacag tacaccttcg ccgccatcaa
    31861 tttgcatggc gcattcaaag attttatcgg gcgtcggtga ggtgaaaatt tcgcccggac
    31921 aggcccccga aagcatcccc tgaccgatat aaccacaatg catcggctcg tgtccgctgc
    31981 cgccacccga cagcagggcg actttacctg caacgggggc atcagatcgg gtgacataca
    32041 ccggatcctg gtgcagtgtc agagatggat gcgctttcgc tagtcctgcc agttgttcgt
    32101 ccagtacgtc ttgcacatca ttgatcaatt ttttcattat tttgctccag caattacggt
    32161 agggcatgga tgatgttcaa cgacacggcg accactgact gccgatgaat ccattgtgca
    32221 tcaggcaagg gaaaagaaaa ttagcccaaa ttatgtttca tagtgaaaca tattctttaa
    32281 tgaatgttcc atattgaaac ttttacgtgt attaatactt aaaattgcga gccggaacac
    32341 cttttgtcat aagggatgcg ggatatgagt agcgctttta acaacgatgg tcgggacata
    32401 tctcccttaa ttgcaacctc ctgggagcga tgcattaagc tgatgaaacg ggatacatgg
    32461 aacgtaccac accaggccca gggcgtgaca tttgcgtcta tttatcggcg taagaaagcg
    32521 atgctgacgc tcgggcaggc tgcgctggaa gatgcctggg aatatatggc accgcgagcg
    32581 tgtgcgctgc ttatcctcga tgaaaccgcc tgcattctca gccgtaatgg cgatccgcaa
    32641 accttgcagc aactaagtgc actgggattc aatgacggca cgtattgcgc cgagggaatt
    32701 attggtactt gtgcgctatc gttagcggct atctctggtc aggccgtgaa aacgatggcc
    32761 gatcaacatt tcaaacaggc actctggaac tgggcctttt gtgcaacgcc gttgtttgac
    32821 agcaagggcc gattgacggg aacaatagcg ctggcgtgtc cggttgagca aactaccgca
    32881 gctgatttgc cgttgacgtt ggcaatcgca cgcgaggtcg gaaatttact gctgacggac
    32941 agtttgctcg ctgaaactaa ccgtcattta aatcaactta atgccctgtt agaaagtatg
    33001 gatgatggcg tgattagctg ggacgagcag ggtaatttgc aatttattaa tgcccaggcg
    33061 gcgcgggtct tacgccttga cgcgacggca agtcagggac gggcaatcac tgaactctta
    33121 acgttacccg ccgtattgca acaagcaata aaacaggcac atccgctcaa acacgtagaa
    33181 gcaacctttg aaagccagca ccagtttatt gatgcggtga taacccttaa accgataata
    33241 gaaacgcagg gaaccagctt tattttgttg ctccatcctg tggaacagat gcggcagtta
    33301 atgaccagtc aattaggaaa agtcagccat actttcgctc atatgccaca agaggatccg
    33361 caaacccgcc gtttgattca ttttggtcgc caggcggcgc gcagtagctt tcctgtcctg
    33421 ctttgtggag aagagggcgt gggcaaggca ctgctaagtc aggcaattca taatgaaagt
    33481 gagcgtgcag cagggcctta tatcgccgtc aattgtgagt tatatggtga tgcggcgctg
    33541 gcggaagaat ttattggcgg cgatcgcacg gacaatgaaa atggtcgttt gagtcgactg
    33601 gaactggcgc acggcggcac gctgtttctt gaaaagattg aatatctggc ggtggagtta
    33661 cagtctgctt tgcttcaggt tatcaagcag ggtgttatca cgcgactgga tgcgcggcgt
    33721 ttaataccca ttgatgtcaa agtgattgcc acaacgaccg cggacctggc aatgctggtg
    33781 gaacaaaatc gttttagtcg ccagctgtat tacgcgctgc atgcatttga aattaccatc
    33841 ccgcctctgc gtatgcggcg tggcagcatt ccggcgctgg tgaataacaa attacacagt
    33901 cttgaaaaac gcttctctac gcggctgaaa attgatgacg atgccctcgc tcgcctggtt
    33961 tcctgtgcat ggcctggcaa cgattttgaa ctttacagcg tcatcgagaa tcttgctctg
    34021 agtagtgata acgggcgcat tcgcgtcagt gatttacctg aacatctctt taccgagcag
    34081 gcgacagatg atgtcagcgc cactcgcctt tccaccagtc tgtcatttgc ggaagttgaa
    34141 aaagaggcaa ttattaacgc agcccaggtc acaggcggtc gcattcagga aatgtcggct
    34201 ttacttggaa tcggccgcac tacgctgtgg cggaaaatga agcaacatgg cattgatgca
    34261 gggcagttta agcgccgtgg atagcgggag agtatgtact ttcccgctat gaattgctaa
    34321 ttaatagcga ccatgactgc cgtgatttgg tccaccattt gaaggtggca ttggcttacc
    34381 ggagggcgca ggaccacgag ggccatcgct gtggcggtga tgatggtggg gaacacaagc
    34441 acttaacagg ctcgcaaaga caattgctaa caggctgaag gtaaatgttt ttttcatgac
    34501 aaactcttga acggttaatg cactgtgatt gttatcacat aaaaggcaca ttgttaatat
    34561 tgccaggagt ctgattatga ttttcgataa ctcagaattg aaagaagccg cgtagcaggc
//
LOCUS       DAZGHF010000040        34429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGHF010000040 DAZGHF010000000
VERSION     DAZGHF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.040"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(57..1916)
                     /gene="gss"
                     /locus_tag="ACHK3A_002013"
     CDS             complement(57..1916)
                     /gene="gss"
                     /locus_tag="ACHK3A_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO8740093.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFSVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2121..2987
                     /gene="yghU"
                     /locus_tag="ACHK3A_002014"
     CDS             2121..2987
                     /gene="yghU"
                     /locus_tag="ACHK3A_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO8740094.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3110..3358)
                     /gene="hybG"
                     /locus_tag="ACHK3A_002015"
     CDS             complement(3110..3358)
                     /gene="hybG"
                     /locus_tag="ACHK3A_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO8740095.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3371..3712)
                     /gene="hypA"
                     /locus_tag="ACHK3A_002016"
     CDS             complement(3371..3712)
                     /gene="hypA"
                     /locus_tag="ACHK3A_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO8740096.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3705..4193)
                     /gene="hybE"
                     /locus_tag="ACHK3A_002017"
     CDS             complement(3705..4193)
                     /gene="hybE"
                     /locus_tag="ACHK3A_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO8740097.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMLLSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4186..4680)
                     /gene="hybD"
                     /locus_tag="ACHK3A_002018"
     CDS             complement(4186..4680)
                     /gene="hybD"
                     /locus_tag="ACHK3A_002018"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO8740098.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4680..6383)
                     /gene="hybC"
                     /locus_tag="ACHK3A_002019"
     CDS             complement(4680..6383)
                     /gene="hybC"
                     /locus_tag="ACHK3A_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO8740099.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6380..7558)
                     /gene="hybB"
                     /locus_tag="ACHK3A_002020"
     CDS             complement(6380..7558)
                     /gene="hybB"
                     /locus_tag="ACHK3A_002020"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO8740100.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSAMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICTYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7548..8534)
                     /gene="hybA"
                     /locus_tag="ACHK3A_002021"
     CDS             complement(7548..8534)
                     /gene="hybA"
                     /locus_tag="ACHK3A_002021"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO8740101.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGSGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8537..9655)
                     /gene="hybO"
                     /locus_tag="ACHK3A_002022"
     CDS             complement(8537..9655)
                     /gene="hybO"
                     /locus_tag="ACHK3A_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO8740102.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9844..10131)
                     /gene="yghW"
                     /locus_tag="ACHK3A_002023"
     CDS             complement(9844..10131)
                     /gene="yghW"
                     /locus_tag="ACHK3A_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO8740103.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10250..11137)
                     /gene="yghX"
                     /locus_tag="ACHK3A_002024"
     CDS             complement(10250..11137)
                     /gene="yghX"
                     /locus_tag="ACHK3A_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO8740104.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQSYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11294..12334
                     /gene="gpr"
                     /locus_tag="ACHK3A_002025"
     CDS             11294..12334
                     /gene="gpr"
                     /locus_tag="ACHK3A_002025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO8740105.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNAIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12374..12868)
                     /gene="yqhA"
                     /locus_tag="ACHK3A_002026"
     CDS             complement(12374..12868)
                     /gene="yqhA"
                     /locus_tag="ACHK3A_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO8740106.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13059..13943
                     /gene="yghA"
                     /locus_tag="ACHK3A_002027"
     CDS             13059..13943
                     /gene="yghA"
                     /locus_tag="ACHK3A_002027"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO8740107.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAITDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     ASYVTAEVHGVCGGEHLG"
     gene            complement(13982..14407)
                     /gene="exbD"
                     /locus_tag="ACHK3A_002028"
     CDS             complement(13982..14407)
                     /gene="exbD"
                     /locus_tag="ACHK3A_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO8740108.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTV
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14414..15148)
                     /gene="exbB"
                     /locus_tag="ACHK3A_002029"
     CDS             complement(14414..15148)
                     /gene="exbB"
                     /locus_tag="ACHK3A_002029"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO8740109.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15400..16587
                     /gene="metC"
                     /locus_tag="ACHK3A_002030"
     CDS             15400..16587
                     /gene="metC"
                     /locus_tag="ACHK3A_002030"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO8740110.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLLPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16727..17386
                     /gene="yghB"
                     /locus_tag="ACHK3A_002031"
     CDS             16727..17386
                     /gene="yghB"
                     /locus_tag="ACHK3A_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO8740111.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17426..18325)
                     /gene="yqhC"
                     /locus_tag="ACHK3A_002032"
     CDS             complement(17426..18325)
                     /gene="yqhC"
                     /locus_tag="ACHK3A_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO8740112.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18519..19682
                     /gene="yqhD"
                     /locus_tag="ACHK3A_002033"
     CDS             18519..19682
                     /gene="yqhD"
                     /locus_tag="ACHK3A_002033"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO8740113.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19787..20614
                     /gene="dkgA"
                     /locus_tag="ACHK3A_002034"
     CDS             19787..20614
                     /gene="dkgA"
                     /locus_tag="ACHK3A_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO8740114.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20814..21740
                     /gene="yqhG"
                     /locus_tag="ACHK3A_002035"
     CDS             20814..21740
                     /gene="yqhG"
                     /locus_tag="ACHK3A_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO8740115.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTTNNPDEDM
                     RIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21791..22048
                     /gene="yqhH"
                     /locus_tag="ACHK3A_002036"
     CDS             21791..22048
                     /gene="yqhH"
                     /locus_tag="ACHK3A_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO8740116.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSDANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22090..24309)
                     /gene="ygiQ"
                     /locus_tag="ACHK3A_002037"
     CDS             complement(22090..24309)
                     /gene="ygiQ"
                     /locus_tag="ACHK3A_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO8740117.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            complement(24420..25832)
                     /gene="ftsP"
                     /locus_tag="ACHK3A_002038"
     CDS             complement(24420..25832)
                     /gene="ftsP"
                     /locus_tag="ACHK3A_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO8740118.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPTLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(25907..26644)
                     /gene="plsC"
                     /locus_tag="ACHK3A_002039"
                     /gene_synonym="parF"
     CDS             complement(25907..26644)
                     /gene="plsC"
                     /locus_tag="ACHK3A_002039"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO8740119.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26878..29136)
                     /gene="parC"
                     /locus_tag="ACHK3A_002040"
     CDS             complement(26878..29136)
                     /gene="parC"
                     /locus_tag="ACHK3A_002040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO8740120.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29274..30881)
                     /gene="ygiS"
                     /locus_tag="ACHK3A_002041"
     CDS             complement(29274..30881)
                     /gene="ygiS"
                     /locus_tag="ACHK3A_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8740121.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGIKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(30990..31472)
                     /gene="ygiV"
                     /locus_tag="ACHK3A_002042"
     CDS             complement(30990..31472)
                     /gene="ygiV"
                     /locus_tag="ACHK3A_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO8740122.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(31525..31917)
                     /gene="ygiW"
                     /locus_tag="ACHK3A_002043"
     CDS             complement(31525..31917)
                     /gene="ygiW"
                     /locus_tag="ACHK3A_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO8740123.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32069..32728
                     /gene="qseB"
                     /locus_tag="ACHK3A_002044"
     CDS             32069..32728
                     /gene="qseB"
                     /locus_tag="ACHK3A_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO8740124.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGDPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32725..34074
                     /gene="qseC"
                     /locus_tag="ACHK3A_002045"
     CDS             32725..34074
                     /gene="qseC"
                     /locus_tag="ACHK3A_002045"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO8740125.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHTMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(34120..34428)
                     /gene="ygiZ"
                     /locus_tag="ACHK3A_002046"
     CDS             complement(34120..34428)
                     /gene="ygiZ"
                     /locus_tag="ACHK3A_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO8740126.1"
                     /translation="MFEALLYIMLTYWLIDAFFAFNKYDWMLESGDDICSIPSVSGED
                     RILQAMIAAFFLLTPLIIIILRKLFVREMFEFWLYVISLVICLVCGWWLFWGRFIFCY
                     "
BASE COUNT         8437 a         9216 c         8695 g         8081 t
ORIGIN      
        1 attaatcccc gtactgatta ttcttcataa tcagtacggg gttgctacaa catgtattac
       61 tctttcacca caattagcgg ttcaatatca ctctcttttt tgatgaccag tgattcatca
      121 ccgcgcaaac acgtcccacc gtagttgccg ccaacggtga aggtacatac ctgaatgtat
      181 ttaccgtcca ctttcggcaa acaccacagt tgctgataga tgtttttctg ctcggcaaat
      241 ttaccgctgg ttttgtccag cacctcttca tgatggctga cgaggtcgat gttactgcca
      301 cagcgaccgg cgatcggttt cactgcataa ccggttttca ccagttcatc attcacggag
      361 aaatcggtat ccagcaggta acgatggtgc gggaacagcg accagaggat cggcagaatc
      421 gctttgttgc cggggatcac tgtccacaga ggttcaaaga ccagcacttc cgggcgcagc
      481 aatacgtcga taagacgcac ttcattttgc ggatgaccgg tacggatcgg caccgcagca
      541 aactcacggt cgctaacttc acgaatctga tcaaacgcgg tttcccacgc ccaggttttc
      601 cacacgcagt taaccagtcg cccttcccca tcaatcagtt gcccggcagc atcccagccc
      661 agttcatcca gcccgcgcaa gatacgcgtt tcaaagcccg cctggtgcag cgcctgctcc
      721 ataaactgcg cgtgatagtt ttcctcgata tctttgtcct gcatgatatg gacaaacgga
      781 cgtgcacgac tgtgtttcca ggcaccagcc agttcgttaa tcagcccttc cgccggattg
      841 aagccgttgc ctttatagcc ctgctccgcc caacgttcga ggatcaagcc agcttcggta
      901 tgacaggagg cggagtcggc gttgtattcg tagaccttca ggccacgttc gtccatacag
      961 aaatccatac gaccggtgat catatggtga cggcgacgct gccaggagag acgcaaacgt
     1021 ggccagagga tttttgggat gtcgaacagc gccagcaggt tgtcatcttt cagcaccttg
     1081 tcggttgcgt gaagatacat caggtgcagt tcgttggtgg ctttaatcag ctcctgctct
     1141 gcgctctcgg taatggtgta gtaatgatac ggatcctgat tgatcacctg accgttggcc
     1201 tgcacatagg cgttttgtag cggatctttt tcatccagcc atttaccgtc aaactggcct
     1261 ttgttttcca ggcgcgctcc gctgattttc aacagctcgc ctgcaatttc cggctgcggt
     1321 aagctgtatt cagtatcttc cgtctggatc atccagccga gaatggtggt gtcatcaaaa
     1381 gtgtctttaa gggtatagca gccgttttcg accaccatct ccagctcgcg cgtccactgt
     1441 tgcccttgcg gtaacgggga atgaatcacg ttctgttccg caatacggac tttgttgcca
     1501 tgcaattggg taatgatggc gacatgacca gtgtctttaa attcaccgcc tttatcccag
     1561 ataagaagcg cacccgcgac cggcgcacgc ggcgagccgt taggaaatgc ctgcaatggc
     1621 aggatgttgt cattaaccac ttcacgcagg aagcgcagcg agaaaatctc ccacgccata
     1681 cccacgtcag tgaagaccac accgtaattc agaaagagaa aacggcgagc aaattcaacg
     1741 cattgccatt tgtggcccat atattcgtcg tcgatatagc tacggaatac ggcgtcatct
     1801 tcgtattcct gcggatcgag agaactgtaa tctgaagagt agattgctac cccacctggg
     1861 gcgtagccca ataatgtccc gaacggggca tcctggctgg tcgttccttt gctcatcact
     1921 ttacctttaa caatacaacc taagcagttg gcgtaattgt tgtgctctga ttacctgctc
     1981 agcgacatta accggacaga ggtcagacta atcatacacc tcatcgcagc ggctggcgca
     2041 aagggttaaa aaattcacat tctgtacagc aagtgacctg ctacactgct tcaacactac
     2101 cactcagaag gcaactcact atgacagaca acacttatca gcccgcgaaa gtctggacgt
     2161 gggacaaatc cgccggcggc gcgttcgcca atatcaatcg cccggtttct ggtccgacgc
     2221 atgaaaaaac gctgcccgtt ggcaaacacc cattgcaact ttattcgctg ggaacgccga
     2281 acggtcagaa agtaacgatt atgcttgagg agctgctggc gctgggcgtt actggtgcag
     2341 agtacgacgc ctggctgatt cgtattggcg atggcgatca attctccagc ggctttgtcg
     2401 aagtgaaccc aaactcgaag atcccggcgc tgcgcgatca tacgcataat ccgccgatcc
     2461 gcgtgtttga gtctggatcg attctgcttt atctggcgga gaaatttggc tacttcctgc
     2521 cgcaggatct ggcaaagcgt actgaaacga tgaactggct gttctggcta cagggggctg
     2581 caccgttcct cggcggtggt tttggtcact tttaccatta cgcgccggtg aaaattgagt
     2641 acgccatcaa tcgctttacc atggaagcca agcgcctgct cgacgtgctg gataagcaac
     2701 tggcgcagca taagtttgtt gcaggcgatg agtacaccat tgcggatatg gcgatttggc
     2761 cgtggtttgg caacgtggtg ttaggcggcg tgtatgatgc cgcagagttt ctggatgcgg
     2821 gaagttataa acacgtacaa cgctgggcga aagaagtggg cgaacgtccg gcagtgaagc
     2881 gtgggcgtat tgttaaccgc atcaacggac cgctgaacga gcagttgcat gaacgccatg
     2941 acgccagtga tttcgagacg aatacggaag ataagcgtca ggggtaaggg ttggtattcg
     3001 tcgcagcaag ccatccaggc cggataaggc gttcacgccg catccggcaa tcgagcacaa
     3061 tgcctgatgc gacgctgccg cgtcttatca ggcctacaaa agctaatcat cacgcgctgg
     3121 taatgtcgta atccatttgg cgcagcgcgt ctaatgtggc tttggcttca tcttcgtcga
     3181 tgatgctcat ggcaaatccg acgtgtacca gtacccactg gcccagcaga tcggcagggt
     3241 taccttcaca aatcagggcg atattcacgt cgcgcttgat accacatact tcaacctgcg
     3301 caagctggtg aatatcttca ccgacagcca gcacctggcc tggaacgcca atacacattg
     3361 ttaactccgg ttattcaact tcaatacttt tgacgattag cgaatcgccg gtatcgacac
     3421 gcaaccgctc gccatgacag atcggacact gcgcatcgtg ctgatgaatc tccaccacct
     3481 ggctgcaatc ccagcaccag gcctgggcgg gtttatagac gatatgtaaa tcgcaccctt
     3541 gcgccaccgt tccgtggcag acaatttcaa aactaaaacg gacggcgctc tcctcaacgc
     3601 aggagagcgc accaatttcc agccacacgg cggtgacgcg cttaacatcg tgctgctccg
     3661 cctgccgttg gataatttca acggcgctct ggcaaagcga caactcatgc attttcgcca
     3721 ctcctgcggc caaatagcag ggcacgacgc cctgcgtgtg gtacatccgg attggtgact
     3781 ggcagcgaaa ggagcattcg ggcgcagtca tccgtcagcc gttggccctc ttcaatcgac
     3841 atgctgtgcg aaagcggcga catcagcgag caggagaggt attgcgaaac accatccagt
     3901 tcaccaacag taaaggtcat agtgccatac ggcagttgca gaccaatttt ttcactcact
     3961 ttgcgcagcg gccagagttg atccgggccg gggaagataa ctgcactcag catccacggg
     4021 gtgatcacac accccgtcca ctgaccttcg aacagcgtaa agtcagaaac atacaccggc
     4081 attgaaggat gcagaaaaga gagatcatgc atcgaacgcc gggcaatttc ttcaaaagct
     4141 gcctgtactt gcgccttcgg ggaggtctgg aaacctgcta tctcttcagt catgaatcgc
     4201 ctcccgtggg atggcttcca cgcctgattc acgcagcgca gccagaacct gctcaagcgc
     4261 aggttcaatc atcgcttcga ccgtcggcgt caagccaatg tgcggctcca gtgattccgg
     4321 gatcacgccg accagggtca gctttttcgg aaactcaccg gtgaagcgca gggccgacaa
     4381 tacgtcggcc aggccaagct gatgcggaga gattttgttg gtaaacaacg ccggaacttc
     4441 ttcatctcgc aggatcatca tcgttcccgg cgcgttcttt ttcgagacaa tggcatccgc
     4501 aataatcaga tgatcgcgat ttgccatgtc gccaagcagc tccattcccg ccgttccgcc
     4561 atcgaggatc tcaacataat ccggcagaat gtatcgttgc tctaacgctt caacaattcg
     4621 cacaccgatg gcttcatcgg tcagcaaaat attgccaacc cctaagacta aaatacgcat
     4681 tacagaacct tcactgaaac cacttcgttg ccgtcggcat ccactacgtg taccgcacag
     4741 gccatgcacg ggtcgaagga gtgaatggta cgcaccactt ccagcggttt attcggatcg
     4801 gcaaccggtg tacccaccag cgactgctcg taaggaccga cgtcatcatt gaagttacgc
     4861 gggccagagt tccaggttga tggaacaact gcctggtagt tactgataat accgtctttg
     4921 atcaccatcc agtgagagag cataccgcgc ggtgcttcaa ggaaaccaac gcctttgaac
     4981 tcacctgtag ccggaatgtt cggcttcaca aaggtggtgt gatcgccttt gccgatattg
     5041 gtgatcagtg cactgtattg attttgcagg atatcctgca attcgcagca gtgaacggta
     5101 cgaccaataa tacggcccag cgtggagtgc agctgcgcca cttccagcgt gttgccagtc
     5161 agtttctgat aaatcgcaac gatttcattc agtttgttct gggtagactc gcgtcctgcc
     5221 gccagtttca ccagcatgtt agccagcgga cccacttcta ccgttttgcc gtagaaagtc
     5281 ggtgatttca cccaggaata tttaccgtcg tcagaccaac catcataagc cggaatggtg
     5341 gtaccttccc acggtgcctg cggcgcttcg tctttatacc aggagtgctt cgcgctctcc
     5401 tgaatgcctt taatcaggta ttcatcggag tgggaagtga tcgggcgata cgaggacaga
     5461 tccgcattct caatgtagcc gcccgggaac aggaagctgc cgtttttgct gtcggtcggg
     5521 aattccggca cgctcaggta gttcaccgca cctttaccgc gcgtcagcca ttccgggtag
     5581 aacgcggcga taactgcggt atcaaccttg taaacctgct caacaaagtc gctcagttta
     5641 tcgatgaaag acttgatgta catcaggcgc tcaaggttca gcacgcccag accgtcgagg
     5701 ttgatcgggt tagcgacccc acctactgcc aggttctgaa tgtgcggcgt tttaccgccc
     5761 agcagcgcca cgacgcggtt agcgtcacgc tggcactcca gcgcctgcag gtagtgcgct
     5821 accgcaatca ggttcacttc cggcggcagt ttcatcgccg ggtgacccca gtagccattg
     5881 gcgaaaatcc ccaactgacc gctggcaacc agatctttaa tcttgttctg aactttggtg
     5941 aactcttccg gactgttcag atgccaggtc gaaacgcctt tcagcatttc agaggctttg
     6001 gttgggtcag cttgcagtgc agaagtgatg tccacccagt ccagcgccga gagctgatag
     6061 aaatgaacga tatggtcatg cgtggtgtgc gcagccagaa tgatgttacg gatgtattgc
     6121 gcgttaaccg gaacgtcgat attcagtgca ctttctgccg cacgaacgga agagagcgcg
     6181 tgagtagtcg tacatacgcc acagatacgt tgcacaatca tccatgcatc gcgcggatcg
     6241 cggtttttca cgatctcttc catgccgcgc cacatggtac cggaagccca tgctttcgaa
     6301 acgacgccat tttcgatttc gcaatcgatg cgtaaatgcc cctcaatacg ggttaccgga
     6361 tcaatagtaa ttctctggct catgctttgc tcgcctcatg acgattatga tcgttttgtt
     6421 ttaaaggagg aagtatcggc agtagacgaa tgagtacgat gtaagtgcaa atctcaatag
     6481 ccacaaaacc aatagaaatc aacagttctt cccaggtcgg gaagtaggcg taaccgccgc
     6541 ccgggttgaa tgccaccagc gaataggtca gacgccaggt tgcacaacct aacagcgcgc
     6601 tcagtgctga caggaacagc atgcgggaat cattacgcag cttcgccaca cgcagaacga
     6661 ccagcgggaa gagcatcagc aggacttcaa tccagaacat cgcggagtag aagtcaccgg
     6721 caaacgctaa cgacagcttg tcgcgataga taagctcgcc aaagcgcagc acgatgaaaa
     6781 tcgccagcaa cacactgatg gtgttggtca gcttgacgaa caggctcttt tcatctggac
     6841 cattgccacg cagacccgcc tgcaccaacg aaccttcaaa gatgacaatc gagaagccca
     6901 tgatgaacgc cgtcagcagc gagaacagcg gcaacatttc atagctctgc cacaacggat
     6961 gcactttgta gcccgccgag atcatcagcg accccattga agactggtgc atagttggca
     7021 gcagcgcacc gagcgcgata atgaagaaca tcaccttgtt cagacgctgt agcgacacct
     7081 tccagcccaa tcgttcaaac aatgccggag caaactccag tgccatcacg ccgatgtaga
     7141 tggtcataca gaccgccgtc tcgaacagta ccgagttcac gttgaagtga cccggaatgt
     7201 agaagtacgg caggttccag tagcgaccaa cgtcgatggt gatcgacaag ccacccagtg
     7261 agtaaccgaa cagactcgcc aacagcgccg gacgcaccag cggatggtat tgcccacggt
     7321 taaagacgta taccgcccac gccagcgccc agccgccaca ggcaaagccg gtgccgatca
     7381 acaggtcaaa cgcgatccac acgccccacg ggaagccgcc gttcaggtca gagacagagc
     7441 ccagaccgaa caccagacgc ttcacaatca ggagcataca gatgacgatt aacggtccaa
     7501 aaatcatgac cggtttactg atgattttgc cgcccagcgg ttgtggatca tgactcatga
     7561 tcgtctcctc cgtcgtgatg gtcgtttttg gtgttgcgac gaaccagcac ggttaagccc
     7621 gccagcacag ccagtggtag catcatgcct ttatacaggg tgtgttgaat attttcggaa
     7681 cgcgcaccgg tagaaagatc gtccagtttc ggcaggtcga gattttcgta aggcacaccg
     7741 gtcagtacca gaacctgagt accgccgccc tctttctcgc cgtacagatg cggataatat
     7801 ttcggcaccg tatgcaggta agtgtcgcca gatttcagcg tctgacgcgg atagtggtat
     7861 tcgctgcctg gcttcagcgc cagacgtttt ttcgcctcag ccatcagctc ttcacgcgta
     7921 ccaaaaatca ccgcgcccgc cgggcacact tctacgcagc caggcagacc gcctttatca
     7981 agacgttcca cacctttctg gttgcacagt tcacacttat gcagcgcacc aaatgggttg
     8041 ttgtagtcgt acttcggcac gttgtatggg caggcgacca tgcagtaacg gcagccagtg
     8101 cacacatctt tgtcgtaatg gacaatgccg gttttcggat cttttttcag cgcagagacc
     8161 gggcacacag agacacaatt cggatcgacg cagtgcatac actgtttctt aatgtacgcg
     8221 tagccgttct cctcctggtc tttgttgacc cccgagccgc tggtccacac ctgaatgatg
     8281 ttattggtgt acggcgacag tttgtcgttg ttcgaccagg tctgttcccc ttgcgggtta
     8341 cgttcaggga aattgatatc ctgacacttg gtgacgcaag cctggcagcc aacgcacaag
     8401 gtcgagtcgt acaacatccc cagcgatccc ggaattggcg ggcggttttc agcagccgca
     8461 tgactgacag acggcagcgc gcccgtcagc aatgccccgc aggaggctgc tttaataaaa
     8521 ttacgtctgt tcacggttat tctccccgtg agtcagcgtt atctttcttt tgctgacgac
     8581 ccagttcacg caccgccatc acgctgacac cggcaaccag cccaaccaca ccgccgagca
     8641 aaccaatagc gcccgcagag acgttgccgc cttctttagc gttaacgtcc ggtttctgtg
     8701 aacgcggagt ctgattttcg acgttggcaa gctgatggat gcctttatgg aagccgatac
     8761 cttcttcgtt acagccatag cacgggtgac caatcgctac cggccacacg ccgccaacgt
     8821 cgcagaattg cagcgttgag cagttgccgt atgtttctgg ccctttacag ccgaggtggt
     8881 acaggcacca gccttcgcgg tggccttcat caccgaactc tttggcaaaa cgaccagcat
     8941 cgaagtgcgg gcgacgttcg cagtgttcgt gaatcagacg gccataggcg aaggtcggac
     9001 ggtttttgtc atccagtttc ggcggcttgc cgtaagtgat gatgtgcgca acggtcgcga
     9061 ggaagttgtg cgggttcggc gggcagcccg gaatattgat aacggttttg cctggcagaa
     9121 cttcttgcag gctgactgcg ccagttgggt taactccagc ggcggcaaca ccgccccacg
     9181 cagagcagga accgatagcg ataatggctg ctgcgccttc cgccgctttg cggatgtgat
     9241 ccacaatcgg ctcaccggca accatgcaat aaataccgtt atcttttaat gggatggaac
     9301 catccaccac taacacatac tgccctttgt acttctcgag agcgttatgt ttgttctctt
     9361 cgacctggtg accgaaagca gcagaaagca cctcgtgata ctccagagag atagtctcca
     9421 gcacgaggtt ttctaccgtt ggatgcgttg cacgaagcag agattccgta caaccggtgc
     9481 actcctgcgc gccaatccag ataactggcg gacgctgcgg gttagtaacc gattcggcca
     9541 tctctgcagc ggctttgcta cttaacccca tggtggcggc taatgctgca caaagcttca
     9601 tgaaatcacg acggttaatg ccgtgagaat ggatgagggt gttatctcca gtcatttata
     9661 gttattccgt tgcgaagacc tggcatatat tttgcctcaa tcgcaaaatc aataatgcga
     9721 tcgatgcgcc atttaccaca catttattat ggttatcgat atcatgatac tgcggcgggg
     9781 cgctaaaacg aagggaagaa gcattagtgt aattaattaa agcaagataa tacgtatgtt
     9841 tgatcaattt tcgaggcgat aaccggccat aaaaaagcgc aacgtagaac aggaattatt
     9901 ctcacgaaag aaatccatta ccatctcttt atccagtcca tagcggcgcg tcagaatacc
     9961 cgcttcccag gcgctaagct ggcgatctcc ggtcttttcg tacatccggt gtgagtagcc
    10021 taatacaaaa ccgcgtttat aatcggcaca gtattttgtc acattaaccg cactgtcggc
    10081 atgcgttgct tttaatcccg ccattaagcc tttaccaaaa tggttattca tcgttcacct
    10141 cattcgctat atattgccgt atatagcgat attttaggca aagaacgaca tttgcgcacg
    10201 ctattttttc atgcttgttt aagacgtaat cctgtaagag ataaacccat caggagagat
    10261 atttatcgaa ccatttcagc gtcctttgcc aggcaagatc ggcggcagat ttgtcataac
    10321 ggggcgtgga atcattatgg aatccgtgat taacccccgg atatatatac gcctcataga
    10381 ccttattatt ggctttcaac gccgcctcgt aagcaggcca gccctcgttg attcgggtgt
    10441 ccagttccgc gtagtgcaac agtaaaggcg cttcaatctt cgccacatcg gcagtgggtg
    10501 cctgacgacc ataaaacggc accgcgcagg ccagttccgg gtaagcgacc gctgccgcgt
    10561 tcgatacgcc accgccatag caaaatccag taatacccac tttgcctgtc gcttgcggat
    10621 agctttgcat aaactcaatc gcggcaaaga aatcattcat cagtttggtt ggatcaacct
    10681 gctgttgcag ctcacgacct ttatcatcat tacccggata acctccaacg gaacttaagc
    10741 cgtcaggtgc cagggcgata taccccgcct tcgccactcg ccgcgtcaca tcttcgatat
    10801 acggattcag ccctcgattc tcatgcacca ctaccacggc tggcgttttg ccgctcattt
    10861 ttgcgggctt caccagataa ccccgtacct cgccgtgacc atttggcgag ggatacgtga
    10921 tgtactctgc aacaatttcc gggtcggtaa actctacctg agtcgccagc gcataatttg
    10981 gcttgagcaa atcaaacagc gctaatgccg tcatcccgcc caccgcatac ttcgccgcaa
    11041 ggttgaggaa ctcacgtttc gagattttcc cgtgagcgta atagtcgtag taatccagca
    11101 actcttgtgg gaaatctttg gcggttaaac gcggcatcgt tgcactcctc aactggtgtt
    11161 tttttaagca aagcataagc gcgtgttttt gcccagtttt tcgtcactct gtgagccaga
    11221 ctacgggata cgcgctggcg aatcgctaaa ctagaaacat tgtttcgaaa ttgaacgatg
    11281 gaaaggagag gtcatggtct ggttagcgaa tcccgaacgt tacgggcaga tgcagtaccg
    11341 ctattgcgga aaaagtggtt tacgcctgcc cgcgttatcg ctcggtttat ggcacaattt
    11401 cggtcacgtt aacgcgctgg aatcacagcg tgcgatcctg cgtaaagcgt ttgatttagg
    11461 cattacgcac tttgatttag ccaacaatta cgggccgcct ccaggaagcg cagaagagaa
    11521 ctttggtcgc ctgctgcgcg aggattttgc cgcttatcgc gatgaactga ttatctctac
    11581 caaagccgga tacgatatgt ggcccggccc gtacggctct ggcggctcac gtaaatacct
    11641 gctcgccagc ctcgaccaaa gcctgaagcg tatggggctt gagtatgtcg atatctttta
    11701 ctctcatcgc gtcgatgaaa atacgccgat ggaagaaacc gcctctgcgc tggctcatgc
    11761 ggtacaaagc ggtaaggcgc tgtatgtcgg gatctcctct tactcgccag agcggacgca
    11821 aaaaatggtt gagttgctgc acgagtggaa aattccgctg ttaattcatc aaccttcgta
    11881 caatttactg aaccgctggg tggataaaag cggcctgctg gataccctgc aaaataacgg
    11941 cgtgggctgc attgccttta ctccactggc tcagggattg ctgaccggaa aatatctcaa
    12001 cgctattccg caagattcac ggatgcatcg tgaagggaat aaagttcgtg gtctgacgcc
    12061 gaaaatgctc accgaagcca acctcaacag cctgcgctta ttgcatgaaa tggcacagca
    12121 gcgtggacaa tcaatggcgc aaatggcgtt aagctggttg ctgaaagatg agcgagtgac
    12181 gtcggtattg attggagcca gccgcgcgga gcaacttgag gagaacgtgc aggcgctgaa
    12241 taatctgaca tttagcaccg aggagctggc gcagattgat cagcatatcg ccgatggaga
    12301 gctgaatctg tggcaggcgt cttccgataa atgacctgtt aataacgggc gcgagaaccg
    12361 cgcccgaatc agatcagtga ttatgacgag tcagtcggtc aagatagccc atcacaaatg
    12421 cagagagcac aaacgtcaga tggataatga cgtaccacat cagtttgtta tcagggacat
    12481 ttttcgcatc cataaagacg cgcagtaagt gaatggaaga aattgccaca atcgacgctg
    12541 ctactttgtt tttcagcgac gttgcgtcca ttttccccag ccagttcagc ttctctttgt
    12601 tctcagagat atcgagttgt gagacgaaat tctcgtaacc ggaaaacatc accatcacca
    12661 gcaaaccgcc aaccagcgtc atatccacca gcgacagcaa cacgaggatc aaatctgatt
    12721 ccgccatcga gaagatattc ggcagtacgt gaataatctc ctggaagaac ttcagcgcca
    12781 gggcaactaa cgccagcgaa aggccaaagt acacgggggc aagcagccag cgagaagcat
    12841 acattgcatt ttcaagaaaa cgttccatac attcctgtcg tgtagttaaa acgggcaaca
    12901 gtatatcgca attaagtaaa cagcacgcaa cagcaaatca acatatacgt taaacctttg
    12961 tcgcataatt ctgagggtta gggatcttcc tggttccctt atcaacgcat cgagagcgtc
    13021 tacccttaaa ggacgttgaa cctgaaggga gaaaaatgat gtctcattta aaagacccga
    13081 ccacgcagta ttacactggt gaatatccca aacagaaaca accgacgcca ggcatccagg
    13141 cgaagatgac accggtaccg gattgcggcg agaaaaccta tgttggtagc ggtcgcctga
    13201 aagatcgtaa agcactggtg acagggggcg attccggaat cggtcgcgct gccgccattg
    13261 cttacgcgcg tgaaggggct gacgtggcga tcagttatct tcccgtggaa gaagaagacg
    13321 ctcaggatgt caaaaagatc attgaagaat gcggacgcaa agccgttctg ctgccaggcg
    13381 atttaagcga tgagaaattc gcccgttcgc tggttcacga agcacacaag gcgttaggcg
    13441 ggctggatat tatggcgctg gtcgccggga aacaggttgc cattactgat attgcagacc
    13501 tcaccagcga acagtttcaa aagacctttg ccattaacgt tttcgcgctg ttctggctaa
    13561 cccaggaagc gatccccctg ctaccgaaag gtgccagtat catcaccact tcgtcaatcc
    13621 aggcatacca gccaagcccg catttactgg actatgcggc tacgaaggcg gcaattctga
    13681 actacagccg tggcctggca aaacaggtcg cggagaaagg tattcgggtg aatattgtcg
    13741 cgccaggccc gatctggaca gcactgcaaa tttccggcgg acaaacgcag gataagatcc
    13801 cgcagtttgg tcagaaaacg ccgatgaagc gtgcggggca accggcggaa ctggcccctg
    13861 tatatgttta tctggcaagt caggaggcga gctacgtcac cgcagaagtg catggcgtgt
    13921 gcggcggcga gcatttaggc taaaaaaatg cccggttgtg aaaagcaacc gggcatcatt
    13981 tttatttcgc tttggcagtt tcttcgccga ccagacctat cttcaggtaa cccgcctgat
    14041 gcagcgtatc cattaccttc atcaacgtct cgtaatcgac ggttttatcc gctcggaaga
    14101 agacggtggt gtctttcttg ccttcggtta acgcattcaa cgccgtaatc attgtttcat
    14161 cggtgaccgg atcgttaccg ataaacatcg agttgtctgc cttcaccgac agataaaccg
    14221 gtttttccgg gcgcggctgc ggcgtgctgg tagaagcagg caagttcacc ttcacatcta
    14281 ccgtcgctaa cggtgccgcc accataaaga taatcagcag aaccaacatc acgtcgataa
    14341 acggcgtcac gttgatatca tgcatttcgc cgttatcgtc gaggttttcg ttaagatgca
    14401 ttgccatcgg acattatcct gcgcgtaatt tttgtgcgac acgaaccgga tgcgcagcgg
    14461 cgctggcttc cagatccagg tcacggcttt gcagcaacaa tacctgcgct gcaacatcac
    14521 ccagcatcgc tttaaagccg ccaatctggc gtgcaaatac gttatagata acgaccgccg
    14581 gaatcgctgc cacgaggccg attgccgttg ctaacagagc ttctgcgata cccggcgcaa
    14641 cgactgccag gttagtagtc tgcgtttgcg cgataccaat aaagctgttc atgatgcccc
    14701 agaccgtacc aaacagacca acgaacggcg aaatcgcgcc gatggttgcc agatagccgt
    14761 taccacggcc catttgacga cctactgcgg cgacccgacg ctccagacgg aagctggtac
    14821 gttctttaat accttcgtta tcgtcgctgc cttctgacag ttccagctca ttctgcgctt
    14881 cattgagcaa atgcaggctc aagcttttgc taccaaaatc agcggcgata tcgttggcct
    14941 ggtttaagga acgcgcttca gccagcagtt gctgctcgcg cttaagacga cgcttctgat
    15001 tgaagaactc cacgctctta ctgaagaaga ttgcccaggt gactacggag gccaaaataa
    15061 gcccaatcat cacgcactta acgacaatat cggcgtgctg atacataccc cagacggaga
    15121 ggtccgtctg cattaaatta ttacccactg tgtatctcca ggacgcaagt cacaaaatct
    15181 gcgcataata atatcaaaac gacgtcgaat tgatagtcgt tctcattact atttgcatac
    15241 tgccgtacct ttgctttctt ttccttgcgt ttacgcagta aaaaagtcac cagcacgcca
    15301 tttgcgaaaa ttttctgctt tatgccaatt cttcaggatg cgcccgcgaa tattcatgct
    15361 agtttagaca tccagacgta taaaaacagg aatcccgaca tggcggacaa aaagcttgat
    15421 actcaactgg tgaatgcagg acgcagcaaa aaatacacgc tcggtgcggt aaatagcgtg
    15481 attcagcgcg cgtcttcgct ggtctttgac agtgtggaag ccaaaaaaca cgcgacacgt
    15541 aatcgcgcca atggcgagtt gttctatgga cggcgtggaa cgttaaccca tttctcctta
    15601 caacaagcga tgtgtgaact ggaaggtggc gcaggctgtg tgctatttcc ctgcggggcg
    15661 gcagcggttg ctaattccat tcttgctttt gtcgaacagg gcgatcatgt gttgatgacc
    15721 aacaccgcct atgaaccgag tcaggatttc tgtagcaaaa tcctcagcaa actgggcgta
    15781 acgacatcgt ggtttgatcc gctgattggt gccgatatcg ttaagcatct gctgccaaac
    15841 actaaaatcg tgtttctgga atcgccaggt tccatcacca tggaagtcca cgatgttccg
    15901 gcgattgttg ccgccgtacg cagtgtggtc ccggatgcca tcattatgat cgacaacacc
    15961 tgggcagccg gtgtgctgtt taaggcgctg gattttggca tcgatgtttc tattcaggcc
    16021 gccaccaagt atctggttgg gcattcagat gcgatgattg gcactgcggt gtgcaatgcc
    16081 cgttgctggg agcagctgcg agagaatgcc tatctgatgg gccagatggt cgatgccgat
    16141 accgcctata taaccagccg tggcctgcgc acattaggtg tgcgtttgcg ccaacatcat
    16201 gaaagcagtc tgaaagtggc tgaatggctg gcagaacatc cccaagtagc gcgagttaac
    16261 caccctgccc tgcctggcag taaaggccac gaattctgga aacgagactt tacaggcagc
    16321 agcgggctat tttcctttgt gcttaagaaa aaactcaatg atgaagagct ggcgaactat
    16381 ctggataact tcagtttatt cagcatggcc tactcgtggg gcgggtatga atcgttgatc
    16441 ctggcaaatc aaccagaaca tatcgccgct attcgcccac aaggcgagat cgattttagc
    16501 ggaaccttga ttcgcctgca tattggtctg gaagatgtcg acgatctgat tgccgatctg
    16561 gacgccggtt ttgcgcgaat tgtataacat tgccactttt ggacaatttt gcagacattt
    16621 tattgtgaaa agtcttaaat tgttgcgtcc gggatcaagg cgtcccggac gattcaggag
    16681 tacaataggc agataaaggc ttaaacgctg ttccacagga aagtccatgg ctgttattca
    16741 agatatcatc gctgcgctct ggcaacacga ctttgccgcg ctggcggacc ctcatattgt
    16801 tagcgttgtt tactttgtta tgtttgccac gctgttttta gaaaacggct tgctgcccgc
    16861 ctcatttttg ccaggcgaca gcttgttgat actggcaggc gcattgattg cccagggggt
    16921 tatggatttt ctgcctacga ttgcgattct gaccgccgca gcaagtctgg gctgctggct
    16981 aagttatatt caggggcgct ggttagggaa taccaaaacg gtgaaaggct ggctggcaca
    17041 gcttcctgct aaatatcacc agcgcgccac ctgcatgttt gaccgccacg gtctgctggc
    17101 gctgctggct ggacgttttc ttgcatttgt ccgtacgctg ctgccaacca tggcgggaat
    17161 ttccggtctg ccaaaccgcc gcttccagtt tttcaactgg ttaagcggat tgctgtgggt
    17221 cagcgtggta accagtttcg gctatgcctt aagtatgatt ccgttcgtta aacgccatga
    17281 agatcaggta atgacgttcc tgatgatcct gccaattgcc ttgttaaccg ctggcttgtt
    17341 gggcacgctg tttgtggtga ttaaaaaaaa atactgtaac gcctgacgat tttccccatt
    17401 cccggtagct gtaccgggaa cgtatttaat tcccctgcat cgcccgcatt cttgccgcat
    17461 cttcccccgg cgtcacaccg aagtaacgtt taaactcacg gctaaattgc gatgcgcttt
    17521 catagccgac gcgcatcgcc gccgcgctgg ccttcatgcc gtcgtggatg atcatcatcc
    17581 gcgccttatg cagacggtaa ttcttcaaat actgcaacgg cgaggtgctg gtgacagact
    17641 taaaattatg gtggaacgcc gatacgctca tgttggcttc tgccgccagt tgctcgacgc
    17701 tcaggttttc ggtgtattta ttctcaatcc gtttcagcac gcgactaatc aggctgaagt
    17761 gagtctggcg gctgaccagt gccagtaacg cgccgccgca aggtccggtc agcacgtagt
    17821 acagaatttc gcggatgatc tgtttgccga gaatacgcgc atccagcggt cgctccatca
    17881 catccagtaa ccgctccgcc gcgcataaaa tctcttctga taacgtggca gagttaatcc
    17941 cgctggctgc catcgacggc tggaaatgct catcttcgcc aatgtccatc aacagttcct
    18001 gtaactgcaa aatatcgaca ttgagacgca accctgccag cggcacctct gacgtggcat
    18061 aggtttcgca ctcaaacggc aacggcaccg tcagcaggag atattcatta gcgtcataac
    18121 gaaacacgcg ttcattgata taaccgattt tatgtccgga aaagagaatt atgatgccag
    18181 gctcgtacat caccggtgtg cgtgcgaaag gcgtctcgcc atacaacaaa cgcacatcgg
    18241 gcaacagctc tgacaaacta ttttctttat ttttcagttt attaacttta tccgccagca
    18301 agcggcaaat ctcttcacgt ttcatatcgc gtaatttctt aggaataatg cggcaatttg
    18361 attgtgcgca attttgtagc atttctccag cactctggag aaataggcaa gacattggca
    18421 gaaatgagca ttgagaggta cggcgctggc gaccacaatg aaaaacatca ggcagaccgt
    18481 tctctgccct catattgacc cagcaaaggg agcaagtaat gaacaacttt aatctgcaca
    18541 ccccaacccg cattcttttt ggtaaaggcg caatcgctgg tttacgcgaa caaattcctc
    18601 acgatgctcg cgtattgatt acctacggcg gcggcagcgt gaaaaaaacc ggcgttctcg
    18661 atcaagttct ggatgccctg aaaggcatgg acgtgctgga atttggcggt attgagccaa
    18721 acccggctta tgaaacgctg atgaacgccg tgaaactggt tcgcgaacaa aaagtgactt
    18781 tcctgctggc ggttggcggc ggttctgtac tggacggcac caaatttatc gccgcagcgg
    18841 ctaactatcc ggaaaatatc gatccgtggc acattctgca aacgggcggt aaagagatta
    18901 aaagcgccat cccgatgggc tgtgtgctga cgctgccagc aaccggttca gaatccaacg
    18961 ccggcgcggt gatctcccgt aaaaccacag gcgacaagca ggcgttccat tctgcccatg
    19021 ttcagccggt atttgccgtg ctcgatccgg tttataccta caccctgccg ccgcgtcagg
    19081 tggctaacgg tgtagtggac gcttttgtac acaccgtgga acagtatgtt actaaaccgg
    19141 ttgatgccaa aattcaggac cgtttcgcag aaggcatttt gttgacgctg atcgaagatg
    19201 gtccgaaagc cctgaaagag ccagaaaact acgatgtgcg cgccaacgtt atgtgggcgg
    19261 cgactcaggc gctgaacggt ttgatcggcg ctggcgtacc gcaggactgg gcaacgcata
    19321 tgctgggcca cgaactgact gcgatgcacg gtctggatca cgcgcaaaca ctggctatcg
    19381 tcctgcctgc actgtggaat gaaaaacgcg ataccaagcg cgctaagctg ctgcaatatg
    19441 ctgaacgcgt ctggaacatc actgaaggtt cagacgatga gcgtattgac gccgcgattg
    19501 ccgcaacccg caatttcttt gagcaattag gcgtgccgac ccacctctcc gactacggtc
    19561 tggacggcag ctccatcccg gctttgctga aaaaactgga agagcacggc atgacccaac
    19621 tgggcgaaaa tcatgacatt acgctggatg tcagccgccg tatatacgaa gccgcccgct
    19681 aagcttttta cgcctcaaac tttcgttttc gggcatttcg tccagactta agttcacaac
    19741 acctcaccgg agcctgctcc ggtgagttca tataaaggag gaacgtatgg ctaatccaac
    19801 cgttattaag ctacaggatg gcaatgtcat gccccagctg ggactgggcg tctggcaagc
    19861 aagtaatgag gaagtaatca ccgccattca aaaagcgtta gaagtgggtt atcgctcgat
    19921 tgataccgcc gcggcctaca agaacgaaga aggtgtcggc aaagccctga aaaatgcctc
    19981 agtcaacaga gaagaactgt tcatcaccac taagctgtgg aacgacgacc acaagcgccc
    20041 ccgcgaagcc ctgctcgaca gcctgaaaaa actccagctt gattatatcg acctctactt
    20101 aatgcactgg cccgttcccg ctatcgacca ttatgtcgaa gcatggaagg gcatgatcga
    20161 attgcaaaaa gagggattaa tcaaaagcat tggcgtgtgc aacttccaga tccatcacct
    20221 gcaacgcctg attgatgaaa ctggcgtgac gcctgttatt aatcagatcg aacttcaccc
    20281 gctgatgcaa caacgccagc tacatgcctg gaacgcgaca cacaaaatcc agaccgaatc
    20341 ctggagtcca ttagcgcaag gtgggaaagg tgttttcgat cagaaagtca ttcgcgatct
    20401 ggcagataaa tacggcaaaa ccccggcgca gattgttatc cgctggcatc tggatagcgg
    20461 cctggtggtg atcccaaaat cggtcacacc ttcacgcatt gtcgaaaact ttgatgtctg
    20521 ggatttccgt ctcgacaaag acgaacttgg cgaaattgca aaactcgatc agggcaagcg
    20581 tctcggcccc gatcctgacc agttcggcgg ctaacgtgca aattctcccg gtggcggtaa
    20641 tgttccgcta ccggactttt cagaaatcat ttattcccct cgcgtcctgc ccattgttac
    20701 tcttccttgt tcaggaatgc caaatataag gacatcatca tgcagagccg gaagctctta
    20761 aaggaacaac tcatctacac ccgggataaa cgcaacggag aggtgaaaaa cagatgaaaa
    20821 taatacttct gtttttagca gccctggcaa gttttaccgt acacgcgcag cctccttcac
    20881 agaccgtaga acaaacagtc cggcagattt atcagaacta taaatcagat gccagtacgc
    20941 cttattttgg tgaaaccgga gagcgagcga taacttctgc gcgtattcaa caggcgctta
    21001 ccctgaacga caatcttacg ctgccgggca atattggctg gctggattat gatccggttt
    21061 gtgattgtca ggattttggc gatctggtgt tagaaagcgt agcgataacc caacctgacg
    21121 ccgatcatgc cgatgccgtt gtgcgctttc gtatctttaa agacgataaa gaaaagacca
    21181 cgcagacact gaaaatggtg gcggaaaatg gtcgttgggt cattgacgat attgtcagca
    21241 atcatggcag cgtcttacaa gcagttaata gcgagaatga aaaaacgctg gccgctatag
    21301 cttcgttgca aaaagaacag ccggaagcct ttgttgccga actctttgaa catattgctg
    21361 attatagctg gccgtggacg tgggtggttt ccgactctta ccgccaggcg gtcaatgcct
    21421 tctataaaac caccttcaag actaccaaca atcccgatga agatatgcga atagaacggc
    21481 aatttattta cgacaatccg atctgttttg gcgaagagtc gctattttca cgcgttgatg
    21541 aaattcgagt cctggagaaa accgccgatt ccgcccgcat tcatgttcgt tttacgctga
    21601 ccaatggcaa caatgaagag caagaactgg ttttacagcg gcgcgaaggt aagtgggaaa
    21661 tcgctgattt tatccgcccg aatagcggta gcctacttaa gcagattgag gcaaaaaccg
    21721 ccgccagatt aaagcaatga gctgagttaa ataacaatta gccggaacaa taaataaaag
    21781 ggaacactat atgaaaacga ttttcaccgt gggagctgtt gttctggcaa cctgcttgct
    21841 cagtggctgc gtcaatgagc aaaaggtcaa tcagctggcg agcaatgtgc aaacattaaa
    21901 tgccaaaatc gcccggcttg agcaggatat gaaagcatta cgcccacaaa tctatgctgc
    21961 caaatccgac gctaacagag ccaatacgcg tcttgatgct caggactatt ttgattgcct
    22021 gcgctgcttg cgtatgtacg cagaatgata aaaaaattcc cggcagcatt ctgttaccgg
    22081 ggatattttt taacgtccaa ccgccgcttt agggcgtttc ttcgcaccag cattctgcag
    22141 gcgagattgc gtagacgacg ctttttttgc cgtagcaggc gtctgacgct gggtcgccat
    22201 cggcgtatgt ttagtcaacg ccggacgggt attgcggttc tggcgacgag cctcacgcat
    22261 ctcttcaatg gttggcgcag gcactaagca atcgcgacgg ctgccaatca gatgcttttt
    22321 gcccatcgct tccagcgcct ggcggattaa cggccagttt gccggatcgt ggtaacgcaa
    22381 caacgcttta tgcaggcgac gctgcttgtc gcccttcggt acaaagacgt cttcactttt
    22441 ataaccaatc ttcgccagcg ggtttttgcc ggtgtaatac atagtggtcg agttagccag
    22501 cggcgacggg tagaagttct gtacctggtc gaggcggaag cggtgctttt tcagccacag
    22561 cgccagattc accatatctt catcgcgcgt acccgggtgc gcggagatga aatacgggat
    22621 cagatactgt tctttacctg cctgtttcga gtaagtatcg aacagctctt taaagcggtc
    22681 atagctgccc atgcccggct tcatcatctt cgataacggc ccttcttcgg tatgttccgg
    22741 ggcaatcttc agataaccgc caacgtgatg ggtcgccagc tctttgatat agcgcggatc
    22801 ttctacggcg atgtcataac gcacaccaga ggcaatcagg atctttttaa tgcctttcag
    22861 atcgcgcgca cggcgataga ggttaatcgt cggttcgtgg ttagtgtcca tatgcggaca
    22921 aatatccgga taaacgcacg acaaacgacg gcaggtttgc tcagcgcgtg gcgatttgca
    22981 acgcaacata tacatgttgg cagtaggccc accgagatcg gaaatcacac ccgtaaaacc
    23041 tggaacagtg tcgcggatcg cttcgatctc attaataatc gaatcttcgg aacggctctg
    23101 aataatgcgc ccttcgtgct cggtaataga acagaaagag cagccgccaa agcagccacg
    23161 cataatgttg accgagaaac gaatcatttc gtaagccgga attcgggcat taccgtaagc
    23221 cggatgcggc acgcgcttgt acggcagcgc aaagacgcta tccatctctt cggtagaaag
    23281 cggaatagca ggcgggttga tccatacata gcggtcgcca tgtttttgca tcaatgcgcg
    23341 ggcgcagcct gggttagttt cgtggtgaag aatgcgcgaa gcatgggcgt acagcacttt
    23401 atcgcccttc actttctcga aagaaggcag caacacgtag gttttttccc acggtttcgg
    23461 gcgcggcggc tgcacggtta cggctttcgc ttcctgcttt ttcggcgcaa caggtttgtt
    23521 atccgcgcac ggcagatctt cgccatacgg atgcgggatc gggtcgattt tcccaggagt
    23581 atcaagacgg gtggaatcca cgccgctcca gcctggcaac gcctctttta cgataatcgc
    23641 ggtattacgc acatcgcgga tttcactaat cggctcgccc atcgccagac gatgcgccac
    23701 ttccaccagc ggacgctcac cgttaccgaa catcagcatg tcggctttcg aatccaccag
    23761 cacggaacgg cgcacggtat cggaccagta atcataatgc gcggtacggc gcagacttgc
    23821 ctcaataccg ccgaggatca ccggtacatc tttccacgcc tctttacaac gctgggtata
    23881 aaccagtgtg gcgcgatccg gtcgcttacc cgcgacgtta tccggcgtgt aggcatcgtc
    23941 atgacgtaaa cggcgatcgg cggtatagcg gttgatcatc gagtccatgt tgccagcagt
    24001 aacaccgaaa aacagattcg gtttacccag acgcataaag tcgtctttgc tgctccagtc
    24061 cggttgggca atgatcccga cacgaaagcc ctgcgcttcc agcatacgac cgcaaatcgc
    24121 catcccgaag cttgggtgat cgacatacgc gtcgccagta accaaaatga tgtcgcagct
    24181 atcccagcca agttgatcca tctcttcacg agacatcggc aaaaacggtg ccggtccaaa
    24241 acaggcggcc cagtactgcg gccaggagaa caggtcgcga tccggttgga tcagggagat
    24301 agagctcatt ttgcttccag aaatgataaa aaaataatca aaggccgggg attataagcc
    24361 ggaacgaaag agaaatcgaa aggtattcca tactcgccct cctcgggcga gtatgaagat
    24421 tacggtaccg gattgaccaa cagttgccct atcgagccac ggtccgccat ttccagcgtc
    24481 tgactgttga agtagaacgg gaagtgcgcc caggaaggct gaccgaaata aaccagcagc
    24541 tccacttgtc cgtcaaccca aacggtatct ttccagcctc tgtcttccgg gaacggcatc
    24601 gcgccattca cgttacggat ctggaacatc acgccttcaa tatggaacgc ttgcggctcg
    24661 tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg cggtgacatc aatacggttg
    24721 acgtcccaca gttgtccatt aatacccggg tcatcaccca gactgatatc gcgactgcga
    24781 atcggcgaac cggccatgat ttcagttggc agcaggcgca tcggaagact gtctgtgacc
    24841 agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg tagaaaccag aatgctggat
    24901 ggctcaaaga agccgcgaat acgatcaaca atgctcgccg cttcaccaca ggtgatcgac
    24961 acttcatcac cgttgctcat atccaccaga atctcgcggc gctcgcccgg tgccagcgaa
    25021 agttgcttca ccgatacagg agcgggcagg aatccctgat cgccagaaat cacatgtaac
    25081 gggcgaccat cgctcatctg tagttgatag cgacgagagt tcgacgcgtt caacagacgc
    25141 aagcgcaccc agccacgcga gacttcaacg tacgggcttt gtacaccgtt aaccagcagc
    25201 gtatcaccaa caaagccgcc gcttcccggt tcgttatatt ccggcgtacc aaagttatcc
    25261 agccgtttat cctgaataat gaccggaaaa tcatccacac cataatggtt ggggataggc
    25321 agcgatttgc tgacttcatc ttccaccagc cacattccgg caaggccgtt atagacctgc
    25381 tgagccgtgc ggttgggagt attggcgtga taccacagag tagctgcgtt ctggcgaatg
    25441 ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc gtgccggacc gcccatcagc
    25501 gggccaggta cctgtagccc ggccaccgtc attgagacat tttctgtcag gcgattgctg
    25561 taaataagct taacatcgtc gcccttccag acgcggatag tcggccccag gtaacgacca
    25621 ttgattcccc aaaccgatgc gcgtgtccct ggcgtaaatg accagtgcgc acgttgtaca
    25681 gtcataaaca gcggttgccc acggcgagat tcaagcagcg tcggaacggg tagcggttgc
    25741 tgttgcccgg ctgcgctggc cttcagggga acagcgcctg cacaaagtgc aatccccgat
    25801 gcctgaatga actgacgccg actgagtgac atatttgctc catgtaaaac tggctaaagt
    25861 aataccgtgc aggaaagtgt tccccgcaaa aatacagatt gccgacttaa acttttccgg
    25921 cggcttcgcg ctctgcgact tctttatcga gctcagcgat tttttgttcc attatcgaac
    25981 gacaatgggc agccaactcg cgaacctgat ctttgccata ctgactgacg tcaattggcg
    26041 gcagcatttc gacaatcacc agaccgttgt gtagccgatt aagattaatc ttattcgaag
    26101 ttgtagagac gcacacggga ataatcggga cgcccgccgc aattgccgcg tgaaatgccc
    26161 cggtcttgaa cggcagcagg ccgcgaccac ggctgcgggt tccttccggg aacatccaga
    26221 tggaaatacg gcgttttttg aagtgattca cgacttccgc aatagtgccg tgagctttag
    26281 tgcgattgtt tctgtcgatc aataagttgc cggttaacca gtacaactgc ccgaagaagg
    26341 ggatccacag caagctcttt ttccctaccg tcactgtcgg cggctgcaca atgttcgatg
    26401 ccgtcaccat gtcataattg ttctggtggt tagcaatata gatagcattt ccgtagcttt
    26461 cagcatccgc aggtttgcgg cactcaactt tcaggccgaa cagcggtgca agacggccaa
    26521 acatatgccc aaaggtggcc acatgtttcg ggttacgcgg gctgaaaagg cagtaaatgg
    26581 agccgaatac acagactaag atgctgtaaa tcacggtaat aataagacga aagatatata
    26641 gcataacacc ctctgaaggt ctgagagact ggcattgtac gtcacctgaa atcaggttag
    26701 ggcattgtta aaagccgctc tctggaaaag cgtattgtta atcgcaacgc atgaataaac
    26761 aacggtttca agggaatttt tatcgtttcc gggggttgaa tacgctgccg gatgacgact
    26821 taacgtttca tccggcgttc cttgcaagcg ggaggaaaca gcgccctccc cggcatatta
    26881 ctcttcgcta tcaccgctgc tggcacggcg aggagagtcg atctcaacac gatcgatacg
    26941 ctgcaaaccg cgcatcaacg taccgcggcg tccacgttcg ccagcgactt tctgtagctc
    27001 ttccggacgc agtttaattt tgcgtttccc aacatgaatg gtcagcgtgc tttgcggcgg
    27061 cagaacgtac agttgcgcaa gaccatcctc gccacgcgcg gcttctgccg atggaatgtt
    27121 gataatcttg ttgcctttgc ccttcgacag ctgcggcaga tcgcttaccg ggaacatcaa
    27181 catacggcct gcctgagtga ttgccagcag catatcggaa gcatcttcaa tcaccaccgg
    27241 cggcataaca tgggcatttt ccggtaaggt gatcaaagcc ttacctgcac ggttacgcgc
    27301 caccagatcg ttaaaggtgc agacgaaacc gtaacccgca tcggaagcca tcagcagttt
    27361 ctgatcgtcg ctttccatca gcatatggtc aacggtcgct ccaggcggca acgttaattt
    27421 gccggtgagc ggctcaccct gaccacgcgc cgacggcagc gtaatcgggt cgatggcata
    27481 gctacgaccg gtggaatcaa caaacactac cggttggttg ctcttacctt tcaccgccgc
    27541 tttgaagcta tcgcccgctt tataattcag gcccggcgcg tcaatatcat ggcctttagc
    27601 gctgcgcacc cagcccatct gcgacagcac aatggtgaca ggttcagacg gcagcatgtc
    27661 gtgctcgctc attgctttcg cttcttcgcg ttcctgcaac ggcgaacgac gttcgtcacc
    27721 gtaggcttgc gcgtctgcct gcagttcttt cttcagcagg ttattcattt tacgctcgga
    27781 agccaaaatg ccctgcaact ggtcgcgctc tttttccagc tcactctgct caccgcgaat
    27841 cttcatctct tccagtttgg caagatgacg cagtttcagt tcgaggatcg cttccgcctg
    27901 ggtttcagta aggccaaacc gcgacatcag cgccggtttc ggttcatctt cattacgaat
    27961 gatctcaatc acttcgtcga tattgagaaa cgccaccagc aaaccttcga ggatatgcag
    28021 gcgcttgagg actttctcca gacgatagtt cagtcggcgg cgcacggtat cgcggcggaa
    28081 caccagccat tcggagagga tttccagcag gtttttcacc gccggacgac catccagacc
    28141 gatcatattg aggttaatgc gatagctctt ttccagatcg gtggtagcaa agaggtggtt
    28201 catcacctga tccatatcca cgcggttgga acgcggcaca atcaccagac gggtcgggtt
    28261 ctcgtggtca gattcatcgc gcagatcgtc aaccatcggc agctttttgt tgcgcatttg
    28321 cgcagcaatt tgctccagta cgcgcgcacc tgaaacctga tgcggcaatg cgctgataac
    28381 caccgcgcca tcttctttct tccaaaccgc gcgcatacgc actgaaccac gtccgttctc
    28441 gtagatttta cgaatctcgg cgcgcgaagt gataatttcc gcttcagtcg gataatccgg
    28501 cccctgcacg atatccagca gctgatcgag cgtggttttc ggctggtcga ttaatgcgat
    28561 tgccgcctga gccacttcac gcaggttatg cggtggaata tcggtcgcca tgccgacggc
    28621 aataccggtg gtgccgttaa gcaaaatgtt tggcagacgg gcaggcagca ttttcggctc
    28681 ctgcaaagtg ccgtcgaagt ttggcaccca gtcagccgtc ccctgcccca gctcgctcaa
    28741 tagcagctcg gaatatttcg acaaccggga ttcggtgtaa cgcattgccg cgaacgattt
    28801 cggatcgtcc ggcgcgcccc agttcccctg accatcaacc agcggataac ggtaagagaa
    28861 cggctgcgcc atcaggacca tcgcttcata acaggcacta tcgccgtgcg gatggtattt
    28921 acccagtacg tcaccgacgg tacgggccga ttttttaaat ttggcgctgg cattcaggcc
    28981 cagctcagac atcgcataca caatgcggcg ctgaacaggt ttcagaccat caccaataaa
    29041 cggcaacgca cggtccatga tcacgtacat ggagtagttt aagtaggcgt tttccgtaaa
    29101 ttcatgtagc gcaaggcgct ctgccatatc gctcattaat tctgattcct caacttattc
    29161 gccagccttc aaaggggcaa tattgccgga gatactacat tatctgccgc gtcgagtcac
    29221 aggagatcag cgtgaatagc gccgccggac gtgctccggc ggcgataaag gcatcaatgt
    29281 gccttgatat acaactcttt gctgtagaca taatcctggg gattatgcag cggaaaaccg
    29341 ccaacgtagg gtttaagcag tttgattaac ggctgatagt agatgggaat cagcggtgcc
    29401 tgctggttga tgatcacttc tgcctgctga tacaacgcat tacgctttgt cgcatcagtg
    29461 atctgcgtgg cctggtttag taaggcgtca tactgcgcat ttttccagtg accgacgttt
    29521 tcttcactat cgcttttgag cgtgttcagg aagctggagg catcattgta cgtcgcatcc
    29581 cacgactgcc gagacagcat aaaatcaccg gctcgtcggg catcaagata ggttttccac
    29641 tccattgtgc gcagcgtcac ctgtgcaccc agccattttt tccattcgga agacaacgct
    29701 atcgcggtct tttcatgcag atcgtacttg ttgtagaaca gctcaaagcg tagcggatga
    29761 gaggcgtcgt atcccgcctg tttcagcaag gcttttgcca tcgcgacacg ctcactcatt
    29821 ggcttttgca gttcatcgaa cgtcgtcgcg ctaaagcctt ttacctctgg cggcgtcagc
    29881 gtggttgcgg gcgttctcaa ccccagtacc ttttgcgcaa taagctgtcg atcaaccgta
    29941 agatatagcg cccgacgcac ccgcacatcg ttaaatggcg gtttctcaag gttaaagttg
    30001 taatattcgc tgttcagacg cgggataatt cgtagctcgc caggcagtga tttttcaatg
    30061 gcgggaattt gctgcgccgg aacccaggtg agatcgacct ctcccgcgcg atagcggtta
    30121 tagccggtga ccgaattatc cagcgccaga tactcaacct gttgcaatac cgtatgttgc
    30181 gcatcgcggt actttggatt tttgcgtgcg gtaatctttt cgttaactac ccatttatca
    30241 agcacaaagg caccgttgta aaccatgttc tctggcttac tccagctatc gccatgttta
    30301 gcgatgacat gatgaggaac cgggaacaac gtcggccagg cgagcatcgt cgtaaaccaa
    30361 ggaactggct gctcaagcgt aacttcaaga gtacgatcat ccgtcgcttt gatccccagc
    30421 gatgtaacat ctgctttacc cgcaacaata gccgcggcat tgttaatgtg cgcctgtgcc
    30481 agatagccag caaaagggct tgccgttttc gggtcaaccg cacgttgcca gccgaggaca
    30541 aaatcctctg ccgtcagagg ctgaccgtct gaccactgca aaccgctacg cagatggaaa
    30601 atatagcgct tgccgccgtc aagtatctcc cagcgttcag cctgagcggg ctgcacctgg
    30661 ccttcaccgt ccatccatac cagaccttca aacagatcca gcacaatctg cgcggcagta
    30721 ttctcctcta ccttttgcgg gtcgagcgtg cctgggtcgc tatgattgtt gtaacgaaag
    30781 acttgttgcg gggcgagcgg tgtgttggcg ggaacgtcag cagcgtagag aggagccgca
    30841 cttaccaggc tgaccagcca taataaattt cgcgtataca tggtattcct taccggagaa
    30901 attataagcc cgacaacaat acgcctgtgg cattgtttgc tcaaacttat cgcgagtgat
    30961 ttggctcaca ctccggtaaa gattccccat cacgccaacg gcacataaat atccgtttcc
    31021 agtcgccgct ctgtcatccc ttcggcaaga ttggtgtagt gaaacaggat cggggctttg
    31081 cgcatttttt cgccgcttgc aggtagccag tggcgaatga tgccccatat cgtgtgtgaa
    31141 atatcgtcca gctcgccaac atggcgggca acagcataac gcccaccggt aagttcgcca
    31201 ttactcacgc cataacgatt atcggggatc ggttcgctaa cgctgccgca gatatcaaag
    31261 cgaaacgctt ctggtgcggt ggtggctggg tcgtcccagg cgacgccaaa agtctggctt
    31321 tggttaacag gagaaagccc cgtctcttta cgccacatga taaatttcgc cacgctgtag
    31381 ttgagcaact cagggctaca gcggtgcggc aacatcgcca cagggattga tgggaaatcg
    31441 ataatattta catccagtgt caggtttgtc attttgcctc cctgcgaaat tattcccggg
    31501 aacgcgtccc gggagcggta acaattacgg atttactttg cggatctgtt tgacgtcaat
    31561 ttcaacagag ttccagtctt tatcgacttc cccctgaatc tcaaccgtat ctttcggcgt
    31621 caccgtcacg ccgttccagc gtttatggtc gatatcaaca ttgatagtac cgctggcatc
    31681 tttgaacacg tagagatcgt cagagatgcg ttcaacgata ttgccgcgta gggttaccca
    31741 ggtgtcgtca cgcagggatt ttgcgctttc tacagtcgtt acgctgccgt tcggcccctg
    31801 gaatcctccg gcctgacttt gcgttgccga tgggccagaa aaacctccct gctctgctgc
    31861 cataaccggt gcactgcaca gggccattac tgcgattact gctgcgaatt ttttcatgtt
    31921 tattactccc tttaatgtct gtttccgagc atttaacaag atagtcctta acaacttctt
    31981 aagggaaaaa aataaaattt aatgctgtac agagcgcgtt acaacacggt ttactggcag
    32041 caaatacggt tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag atgacatgct
    32101 gattggcgac ggcatcaaaa cgggccttag taaaatgggt tttagcgtcg actggtttac
    32161 acaaggtcgt cagggaaaag aggcgctata tagcgcacct tatgatgcgg tgatcctgga
    32221 tttaacctta ccgggcatgg atggtcgcga tattttgcgc gaatggcgag aaaaaggtca
    32281 gcgtgagccg gtattgatcc tgactgcgcg cgatgcgctg gcggaacgtg tagaagggct
    32341 gcgtctggga gctgacgatt atctgtgtaa accttttgcg ttgatagaag tcgccgccag
    32401 gctggaagct ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc gccacggcaa
    32461 cgtcatgcta gaccccggca aacgtatcgc cacgctggct ggcgacccct taacgctgaa
    32521 accaaaagaa tttgccctgc tggaattact gatgcgtaac gctggtcggg tactgccgcg
    32581 caaactgatt gaagagaaac tgtatacctg ggacgaagag gtcaccagta atgccgttga
    32641 agtgcatgtg catcatctgc gacgcaaact cggtagtgat tttattcgta ccgtgcatgg
    32701 tattggctac acattaggtg agaaatgaaa tttacccaac gtcttagtct gcgagtcagg
    32761 ctgacgctaa tctttttaat tctggcctcg gtgacctggc tgctttccag ctttgtcgcc
    32821 tggaaacaaa caacggataa cgtcgatgag ttgttcgaca cccaactgat gctgtttgcc
    32881 aaacggttaa gtacgctcga tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg
    32941 ccaaataaat taaaacacgg tcatgttgat gacgatgcgc tgacttttgc catctttacc
    33001 cacgacggca gaatggtcct taacgatggc gataacggag aagatattcc ttatagctat
    33061 cagcgggaag gttttgctga cgggcaactg gtcggtgaag acgatccctg gcgttttgtc
    33121 tggatgacct cacctgatgg caaatatcgc atcgttgtgg gtcaggaatg ggaataccgt
    33181 gaagacatgg cgctggcgat tgttgccgga caattgatcc cgtggctggt cgcactgcca
    33241 attatgttaa tcatcatgat ggtactactg ggtcgtgaac tcgcgccgct gaacaaactg
    33301 gcgctggcac tacgtatgcg tgaccctgac tcggaaaaac cactaaacgc gacgggcgta
    33361 cccagcgaag tgcgcccact ggttgagtcg ctaaatcaac tgttcgcccg cacacatacg
    33421 atgatggttc gtgaacgacg ctttacctcc gacgcagctc acgaacttcg tagcccgtta
    33481 acggcgctga aagtgcaaac cgaagttgct cagctctctg acgatgatcc gcaggcgcgg
    33541 aaaaaagcgc tgcttcaatt acattccgga atcgatcgtg ctactcgtct ggttgatcaa
    33601 ctgctcacgc tatcgcggct ggactcactg gataaccttc aggacgtcgc ggagatcccg
    33661 cttgaagatc tcctgcaatc gtcggtgatg gatatttacc acacggcgca gcaggcgaac
    33721 attgacgtgc gactgacact caatgccaac ggcatcaaac gcaccggaca accgctattg
    33781 ctaagtttgt tggtgcgaaa tttgctggat aacgccgtgc gctacagtcc acagggcagc
    33841 gtggtggacg tcacgctgaa tgccgataat ttcatcgtga gggataacgg ccccggtgtg
    33901 acaccagagg cactggcgag aattggcgaa cgcttctatc gcccacccgg acaaaccgct
    33961 accggcagtg gacttgggct atcaattgtc cagcgaatcg ccaagctgca tgacatgaat
    34021 gttgaatttg ggaatgcgga acaaggtgga tttgaggcga aagtaagctg gtaacttctc
    34081 tgtctatgag agccgttaaa acgactctca tagattttat taatagcaaa atataaaccg
    34141 tccccaaaaa agccaccaac cacaaaccaa acaaataacg agcgagatta catataacca
    34201 aaactcaaac atttctcgca caaagagttt tcttaagata ataataataa gtggcgtcag
    34261 aagaaaaaat gctgcaatca ttgcttgcaa tatacgatct tcgcctgaaa cggaaggtat
    34321 actgcaaata tcatctcccg actcaagcat ccagtcatac ttattgaaag caaaaaaagc
    34381 atctattaac caataagtaa gcattatgta taatagtgct tcaaatatt
//
LOCUS       DAZGHF010000041        33531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGHF010000041 DAZGHF010000000
VERSION     DAZGHF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.041"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            74..358
                     /gene="pduA"
                     /locus_tag="ACHK3A_002047"
     CDS             74..358
                     /gene="pduA"
                     /locus_tag="ACHK3A_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFO8740127.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            355..1167
                     /gene="pduB"
                     /locus_tag="ACHK3A_002048"
     CDS             355..1167
                     /gene="pduB"
                     /locus_tag="ACHK3A_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFO8740128.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1186..2850
                     /gene="pduC"
                     /locus_tag="ACHK3A_002049"
     CDS             1186..2850
                     /gene="pduC"
                     /locus_tag="ACHK3A_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFO8740129.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPANQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNIKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2860..3528
                     /locus_tag="ACHK3A_002050"
     CDS             2860..3528
                     /locus_tag="ACHK3A_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFO8740130.1"
                     /translation="MEINEKLLRQIIEDVLAEMQTSDKPVSFRAPVSATASSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3543..4061
                     /gene="pduE"
                     /locus_tag="ACHK3A_002051"
     CDS             3543..4061
                     /gene="pduE"
                     /locus_tag="ACHK3A_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFO8740131.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4071..5903
                     /locus_tag="ACHK3A_002052"
     CDS             4071..5903
                     /locus_tag="ACHK3A_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFO8740132.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSYVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGSNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     LGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKGAIHGK"
     gene            5893..6243
                     /locus_tag="ACHK3A_002053"
     CDS             5893..6243
                     /locus_tag="ACHK3A_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFO8740133.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDNHARRSIGTNA
                     ARLVKGIPFRELHS"
     gene            6263..6538
                     /gene="pduJ"
                     /locus_tag="ACHK3A_002054"
     CDS             6263..6538
                     /gene="pduJ"
                     /locus_tag="ACHK3A_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFO8740134.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6562..6984
                     /locus_tag="ACHK3A_002055"
     CDS             6562..6984
                     /locus_tag="ACHK3A_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFO8740135.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            6987..7619
                     /locus_tag="ACHK3A_002056"
     CDS             6987..7619
                     /locus_tag="ACHK3A_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFO8740136.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKPLMQPGQFAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVTVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVT"
     gene            7616..8107
                     /gene="pduM"
                     /locus_tag="ACHK3A_002057"
     CDS             7616..8107
                     /gene="pduM"
                     /locus_tag="ACHK3A_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFO8740137.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRAAIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHDRLLSYVDIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8111..8386
                     /locus_tag="ACHK3A_002058"
     CDS             8111..8386
                     /locus_tag="ACHK3A_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFO8740138.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRQVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8398..9405
                     /gene="pduO"
                     /locus_tag="ACHK3A_002059"
     CDS             8398..9405
                     /gene="pduO"
                     /locus_tag="ACHK3A_002059"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFO8740139.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHCFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9402..10787
                     /gene="pduP"
                     /locus_tag="ACHK3A_002060"
     CDS             9402..10787
                     /gene="pduP"
                     /locus_tag="ACHK3A_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HFO8740140.1"
                     /translation="MNNSELETLIRNILREQLVPATQRAQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKNVSLKLITIIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VVSVAEYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10798..11910
                     /locus_tag="ACHK3A_002061"
     CDS             10798..11910
                     /locus_tag="ACHK3A_002061"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFO8740141.1"
                     /translation="MKTFSLQTRLYSGTGSLNILNRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGIAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIHFNAGD
                     PRAAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLTNALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11907..13250
                     /locus_tag="ACHK3A_002062"
     CDS             11907..13250
                     /locus_tag="ACHK3A_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO8740142.1"
                     /translation="MNVELTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVNVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGNLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLTVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLIPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13253..13807
                     /gene="pduT"
                     /locus_tag="ACHK3A_002063"
     CDS             13253..13807
                     /gene="pduT"
                     /locus_tag="ACHK3A_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFO8740143.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13807..14157
                     /gene="pduU"
                     /locus_tag="ACHK3A_002064"
     CDS             13807..14157
                     /gene="pduU"
                     /locus_tag="ACHK3A_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFO8740144.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14162..14605
                     /locus_tag="ACHK3A_002065"
     CDS             14162..14605
                     /locus_tag="ACHK3A_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO8740145.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTSAVNNNGVTELFALLHTEEGCR"
     gene            complement(14706..15035)
                     /gene="tmaR"
                     /locus_tag="ACHK3A_002066"
     CDS             complement(14706..15035)
                     /gene="tmaR"
                     /locus_tag="ACHK3A_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO8740146.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15207..16265)
                     /gene="yeeA"
                     /locus_tag="ACHK3A_002067"
     CDS             complement(15207..16265)
                     /gene="yeeA"
                     /locus_tag="ACHK3A_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO8740147.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16463..16936)
                     /gene="sbmC"
                     /locus_tag="ACHK3A_002068"
     CDS             complement(16463..16936)
                     /gene="sbmC"
                     /locus_tag="ACHK3A_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO8740148.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17055..18221)
                     /gene="dacD"
                     /locus_tag="ACHK3A_002069"
     CDS             complement(17055..18221)
                     /gene="dacD"
                     /locus_tag="ACHK3A_002069"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO8740149.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVAMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18430..19857
                     /gene="sbcB"
                     /locus_tag="ACHK3A_002070"
     CDS             18430..19857
                     /gene="sbcB"
                     /locus_tag="ACHK3A_002070"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO8740150.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSKFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(19931..21289)
                     /gene="plaP"
                     /locus_tag="ACHK3A_002071"
     CDS             complement(19931..21289)
                     /gene="plaP"
                     /locus_tag="ACHK3A_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO8740151.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(21279..21341)
                     /gene="yoeI"
                     /locus_tag="ACHK3A_002072"
     CDS             complement(21279..21341)
                     /gene="yoeI"
                     /locus_tag="ACHK3A_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO8740152.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(21556..22485)
                     /gene="yeeY"
                     /locus_tag="ACHK3A_002073"
     CDS             complement(21556..22485)
                     /gene="yeeY"
                     /locus_tag="ACHK3A_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8740153.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(22531..23355)
                     /gene="yeeZ"
                     /locus_tag="ACHK3A_002074"
     CDS             complement(22531..23355)
                     /gene="yeeZ"
                     /locus_tag="ACHK3A_002074"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8740154.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(23438..23692)
                     /gene="yoeB"
                     /locus_tag="ACHK3A_002075"
     CDS             complement(23438..23692)
                     /gene="yoeB"
                     /locus_tag="ACHK3A_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO8740155.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(23689..23940)
                     /gene="yefM"
                     /locus_tag="ACHK3A_002076"
     CDS             complement(23689..23940)
                     /gene="yefM"
                     /locus_tag="ACHK3A_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO8740156.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            24223..24273
                     /gene="hisL"
                     /locus_tag="ACHK3A_002077"
     CDS             24223..24273
                     /gene="hisL"
                     /locus_tag="ACHK3A_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO8740157.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    24249..24372
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            24419..25318
                     /gene="hisG"
                     /locus_tag="ACHK3A_002078"
     CDS             24419..25318
                     /gene="hisG"
                     /locus_tag="ACHK3A_002078"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO8740158.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            25324..26628
                     /gene="hisD"
                     /locus_tag="ACHK3A_002079"
     CDS             25324..26628
                     /gene="hisD"
                     /locus_tag="ACHK3A_002079"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO8740159.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            26625..27695
                     /gene="hisC"
                     /locus_tag="ACHK3A_002080"
     CDS             26625..27695
                     /gene="hisC"
                     /locus_tag="ACHK3A_002080"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO8740160.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            27695..28762
                     /gene="hisB"
                     /locus_tag="ACHK3A_002081"
     CDS             27695..28762
                     /gene="hisB"
                     /locus_tag="ACHK3A_002081"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO8740161.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDLANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            28762..29352
                     /gene="hisH"
                     /locus_tag="ACHK3A_002082"
     CDS             28762..29352
                     /gene="hisH"
                     /locus_tag="ACHK3A_002082"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO8740162.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLARRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            29352..30089
                     /gene="hisA"
                     /locus_tag="ACHK3A_002083"
     CDS             29352..30089
                     /gene="hisA"
                     /locus_tag="ACHK3A_002083"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO8740163.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPARRQIPLIKTLVADVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            30071..30847
                     /gene="hisF"
                     /locus_tag="ACHK3A_002084"
     CDS             30071..30847
                     /gene="hisF"
                     /locus_tag="ACHK3A_002084"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO8740164.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            30841..31452
                     /gene="hisIE"
                     /locus_tag="ACHK3A_002085"
     CDS             30841..31452
                     /gene="hisIE"
                     /locus_tag="ACHK3A_002085"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO8740165.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(31547..32524)
                     /gene="wzzB"
                     /locus_tag="ACHK3A_002086"
     CDS             complement(31547..32524)
                     /gene="wzzB"
                     /locus_tag="ACHK3A_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO8740166.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(32671..>33531)
                     /locus_tag="ACHK3A_002087"
     CDS             complement(32671..>33531)
                     /locus_tag="ACHK3A_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFO8740167.1"
                     /translation="DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREG
                     KALYDNLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYL
                     AMRVAYFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLL
                     ANYQSVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQG
                     IMKRIKAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADK
                     VYTRDLFGSD"
BASE COUNT         8499 a         8710 c         8491 g         7831 t
ORIGIN      
        1 gattaagtca gccattcttt ttcctcgctt ttttttatag tcatatcgtc aaaaaaatcc
       61 aggcgaggtc tttatgcaac aagaagcgtt aggaatggta gaaaccaaag gcttaactgc
      121 cgccatagag gccgcagacg ccatggtgaa gtcggcaaat gttttgctgg tcggctacga
      181 aaaaataggt tccgggctgg tgaccgtcat cgttcgtggt gatgtcgggg cggtaaaagc
      241 ggcaacagac gcgggtgccg cagctgcgcg taacgtcggc gaagtaaaag ccgtacacgt
      301 tattccccgc ccacataccg atgtcgaaaa aatcttacca aagggaatta acccatgagc
      361 agcaatgaac tggtcgaaca gatcatggcg caggtcatcg ctcgcgtagc gacgccagag
      421 catgaggttc tcccgaaaac cactcatcaa atacgagaga cggctatggc agaaaaaagc
      481 tgcacgttaa cagaatttgt tggcaccgct attggcgaca ctgtaggtct ggtcattgcg
      541 aacgttgata gcgccctgtt ggacgcgatg aaacttgaaa aacgctatcg ttccattggc
      601 attctggggg cgcgtattgg cgcagggcca cacattatgg ccgctgatga agcggtaaaa
      661 gccaccaaca ctgaagttat tagcattgaa cttcctcggg atactaaagg cggtgccgga
      721 cacggctcat tgatcctctt aggtggcaac gatgtttccg acgtgaaacg cggtattgaa
      781 gttgccctga aagaactcga tcggaccttt ggtgatgtct atgccaacga agccgggcat
      841 cttgaactgc aatacaccgc ccgcgccagc tatgcactgg aaaaagcgtt tggcgctcca
      901 ctgggacggg cttgcggcgt tattgtcggc gctccggctg cggtgggtgt tctgatggcc
      961 gacaccgcgc tgaagtccgc caatgtggat gtcgtggcat acagctcccc cgctcaaggc
     1021 accagctaca gcaacgaagt cattctgatt atctcgggtg actccggcgc agtccgccag
     1081 gcggtcatct ccgcacgcga aatcggtaaa accgtgcttt cgactctcgg cgatacaccg
     1141 aaaaacgatc gcccttccta catctgaatt ctctgaggca gatttatgag atcgaaaaga
     1201 tttgaagcac tggcaaaacg cccggtcaac caggacggtt tcgttaaaga gtggattgaa
     1261 gaaggcttca ttgcgatgga aagcccgaac gatcctaaac cgtcaatcaa aattgttaac
     1321 ggcgtcgtta ccgaactgga tggaaaaccc gccaatcagt ttgacctgat cgaccacttt
     1381 atcgcccgct acggcatcaa tctcgcgcgc gcagaagaag tcatcgcgat ggattcggta
     1441 aaacttgcca atatgttgtg cgatccaaat gtcaaacgta gcgatatcgt tccccttacc
     1501 acagcaatga cgcctgcaaa aatcgtcgaa gtggtttcgc agatgaacgt ggtagagatg
     1561 atgatggcga tgcagaaaat gcgcgcccgt cgaacaccat cacaacaggc tcacgtcacc
     1621 aacatcaaag ataacccggt acaaattgcc gccgacgcag ctgaaggcgc atggcgcggg
     1681 tttgatgaac aggaaaccac cgttgccgtt gctcgctatg caccgttcaa cgccatcgcc
     1741 ctgctggtcg gctcacaggt gggacgtccg ggcgtcttga cccagtgttc actggaagaa
     1801 gccaccgaac tgaaactcgg tatgttaggc cacacctgtt acgccgaaac aatttctgtc
     1861 tatggtacag aacctgtttt caccgatggc gatgataccc cgtggtcaaa agggtttctc
     1921 gcgtcatcct atgcgtctcg cggcttaaaa atgcgcttta cttccggctc cggctctgaa
     1981 gtacagatgg gttacgcgga aggcaaatca atgctttatc tcgaggctcg atgcatttac
     2041 atcaccaaag ccgcaggcgt tcagggtctg cagaatggtt cggtaagctg tatcggtgtt
     2101 ccatcagctg tgccatcagg catccgcgca gtattagcgg aaaacttaat ttgctcatcg
     2161 ctggatctgg aatgcgcctc cagtaatgac cagaccttta cccactcgga tatgcgccgt
     2221 accgcacgtt tcctgatgca atttctgcca ggtaccgact ttatctcttc cggtttttcc
     2281 gctgtgccga actacgacaa catgtttgct ggttcaaacg aagatgctga agacttcgat
     2341 gattacaacg tcattcagcg tgacctgaag gttgatggcg gcctgcgacc agtacgcgaa
     2401 gaagatgtta tcgctatccg caataaagcc gctcgcgctt tacaggctgt ctttgccggt
     2461 atgggactac cgcctattac cgatgaagaa gtcgaagcgg caacctatgc ccacggttca
     2521 aaagatatgc ctgagcgcaa tattgtagaa gacatcaagt tcgcccagga gattatcaat
     2581 aaaaaccgga acagcctgga agtggtgaaa gccctggcgc aaggcggttt taccgatgtc
     2641 gcccaggaca tgctcaacat gcaaaaagca aaactgaccg gcgattatct ccatacctca
     2701 gccatcatcg tcgatgacgg acaagtgctc tctgcggtca atgacgtcaa tgattacgcc
     2761 ggaccggcta caggttaccg cctgcaaggg gaacgctggg aagagatcaa aaatattccc
     2821 ggtgcacttg atcccaacga aattgactaa gggtgagaaa tggaaattaa cgaaaaatta
     2881 ctgcgtcaga ttatcgaaga cgtgctggca gaaatgcaaa ccagcgacaa gcccgtttca
     2941 tttcgcgcac ctgtctctgc aactgcctct tccgcgccag ataccggaaa ttttctgacc
     3001 gaaattggcg aagcccaaca aggcactcag caagatgaag tcattattgc tgtaggcccg
     3061 gcgttcggcc tggcgcaaac cgtcaatatc atcggtattc cgcataaaaa cattttgcgc
     3121 gaagtgattg ccggtatcga agaagaaggc attaaagccc gtgtgattcg ctgctttaaa
     3181 tcgtccgatg tcgcattcgt agcagtcgaa ggcgatcgtc ttagtggttc aggtattgct
     3241 atcggtattc agtcgaaagg cacaacggtt atccaccagc agggacttcc tcccttgtct
     3301 aacctggaac ttttcccaca agcgcctttg ttgacgctgg atacttatcg tcagattggc
     3361 aaaaacgccg cgcgttatgc aaagcgtgag tctccacaac cggtgccgac cctcaacgat
     3421 caaatggcgc ggccgaaata ccaggccaaa tctgccattt tgcatattaa agagacgaag
     3481 tacgtggtca ccggcaaaaa accgcaagaa ctgcgcgtga cgttttaaca aaggaaacct
     3541 tcatgaatac cgatgcaatt gagtcgatgg tcagagacgt gttgaaccgc atgaacagtt
     3601 tacaggatgc agctcctgtt tctgcggtcc ccaacgcgtc aatacagagc gccaaagtga
     3661 cggattaccc gctggcaaat aaacatccgg aatgggttaa aacggcgacc aataaaacgc
     3721 tcgacgattt cactctggaa aatgtgctaa gcgataacgt caccgcccag gatatgcgta
     3781 taacccctga aacattgcgt attcaggccg caattgccag agatgctggt cgcgatcgcc
     3841 tggcgatgaa ttttgaacgc gccgctgagc ttacctcagt accggatgat cgcatcctcg
     3901 aaatctacaa tgcgttacgc ccttatcgtt cgactaagca agagcttatt gccattgctg
     3961 acgatcttga acggcgttat caggccaaaa tttgtgctgc atttgtccgt gaagctgcgg
     4021 aactttatgt cgagcgcaaa aaactgaaag gcgacgatta aggacgaact atgcgatata
     4081 tagcaggcat tgatatcggc aactcatcaa cagaggttgc gctggcaacg ctcagtgcaa
     4141 ctggagagtt gtcgtacgtg agtagcgccc tggcggaaac caccggaatt aaaggtacgt
     4201 tgcgtaacgt ccatggtatt caggaggcgc tcgcacaggc aacgaaaaaa gtcggcatca
     4261 atgttagcga tatttcgctt attcgtatca acgaagcaac tccggtcatt ggtgatgtgg
     4321 cgatggaaac catcactgaa accatcatca ccgaatcaac catgatcggt cacaacccga
     4381 aaactccggg cggagttggg ctgggcgtag ggctcaccat cactccgcaa gagttattaa
     4441 cacgcccggc ggacacacct tacatcctgg tggtgtcatc ggcatttgat tttgccgata
     4501 tcgcaacgat gataaacgcc tccgtacgag ccggatatca gctgactggc gttattctgc
     4561 aacgcgacga tggcgtactg gtcaataacc gcctggaaat tcctctccct attgtggatg
     4621 aagtcctcta catcgatcgt attccgctcg gcatgctggc tgcaattgaa gttgccgttc
     4681 ccgggaaagt tatcgaaacc ctgtctaacc cgtatgggat tgccaccgtt ttcgccctca
     4741 atgctgaaga gactaaaaat attgttcctg tcgcgcgggc gctaatcggc aaccgttcag
     4801 cagtggtagt gaaaacgcct tctggcgacg ttaaagcccg ttcgataccc gccggaaata
     4861 tcgaactgct ttctgccggg cgcacaacgc gggttgatgt agctgctggt gccgatgcca
     4921 ttatgaaagc ggtcggtgag tgcccaaaac tggaaaacgt gacgggagaa cctggctcca
     4981 acatcggcgg aatgctggaa catgttcgcc agaccatggc tgaactgacc aacaaaccca
     5041 gcaacgaaat ttttattcag gatttgctgg cgatagatac ctctgtcccc gtcaacgtaa
     5101 ccggtggtct ggcaggtgaa ttttcactgg aacaggctgt gggtattgcc tctatggtga
     5161 aatccgaccg tctgcaaatg gcgatgatcg ccagtgaaat taagcaaaaa ctgcatgttg
     5221 atgttcaggt cggaggcgca gaagcagaag ccgccattca gggggccttg actacccctg
     5281 gaaccacacg tccactggca attctggact taggtgcagg ctctaccgat gcctccatca
     5341 tcaatcagaa aggggaaata gttgccactc accttgcggg agcgggcgac atggtaacga
     5401 tgattatcgc tcgtgagctg ggcttaaatg atcgttatct cgcagaagaa atcaaaaagt
     5461 acccgttggc aaaagtagaa agcctgttcc acttacgcca tgaagatggc agcgttcagt
     5521 tcttccctac tccgctgtcc cctcatgtat ttgcccgcgt ttgtgtggtg aaacctgacg
     5581 aactggtgcc tattctcggt gatttgacgc tggaaaaagt acgtgctgtc cgtcgtagtg
     5641 cgaaagagcg tgtcttcgta actaatgccc tgcgcgcgct acgtcaggta agccctgccg
     5701 gaaatattcg cgacattccg ttcgtcgttc tggtgggtgg ttcttcgctg gatttcgaag
     5761 taccgcaatt ggtcaccgac gcgctggcac attatcgact ggtcgccggg cgggggaata
     5821 tccgtggtag cgaaggtccg cgcaatgcgg tagctactgg gctcctcatc gcctggcaca
     5881 agggggcgat tcatggaaag taaagagcgt gcaccagcca tcgttgtcat ggagatcggt
     5941 gactgtatca ctacatggga tgaagtgctt cttggcattg aagaggaagg tattcccttt
     6001 cgcattcaac acattccgtc aggtgaagtt atcgatagcg cctggctggc ggctcgtcaa
     6061 tctcccctgt tggttggcat tgcctgcgat caggaaaaac tgattgtgca ttacaaaaac
     6121 ttaccggcat cagcgccact gtttacgctg atgtatcaac aagataacca tgccaggcgc
     6181 agcatcggca ctaacgctgc ccgcctggta aaagggattc ccttccggga actccactca
     6241 taatccacag gagaaacgca gtatgaacaa cgcactagga ctggttgaaa caaaagggtt
     6301 agttggcgca attgaagctg cagatgccat ggttaaatcc gccaacgtgc agctggttgg
     6361 ctatgaaaag attggttcgg gtctgattac ggtgatggtc cgtggcgatg ttggcgcagt
     6421 aaaagccgcc gttgatgcag gaagcgccgc cgcaagcgcc gttggcgaag tgaaatcctg
     6481 ccatgtcatc ccgcgcccac acagtgatgt tgaggctatt ttacctaaat cagcataacc
     6541 ccattcgcta aggagcacag cgtgaagcaa tcactgggat tacttgaagt cagtggtctg
     6601 gcactcgcca tcagttgcgc agatgctatg gcaaaagccg cgtccatcac gctggttggc
     6661 cttgagaaga ccaatggctc aggctggacg ctcatcaaaa tcaccggtga cgtcgcctcg
     6721 gttaatgcgg cgatcgcgac gggagccagt ttcgccgggc agcgaaatgg ccttgtcgca
     6781 cacaaagtaa ttgcccgccc gggagacggc attctgcccc acacaactga agcattgact
     6841 gtgacggttg aaacaagcga aacaccagca acaaccgtca tctcagagat tatcagttgt
     6901 aatttgtgtc ttgacccggc atgtcagcgg caaaaaggtg agccccgctc actttgcctg
     6961 tacgccaata agcgagggga gtagcagtgg ataaacaaac cctgcaggaa acagtgagcc
     7021 agattctgga tgagatgcgc gagcgcccta tcccactggg cgtttccagt cgccatattc
     7081 atctttcaac agcggattat ttgcgtcttt ttcctgaaca accgattagc gagaaaaagc
     7141 cgttaatgca accgggccaa ttcgctgctg aacaggttgt tacgctggag ggaccaaaag
     7201 gcaaattgca caacgttcgc ttgttggggc cgctacgcaa tgcaagccag gtggaaattt
     7261 cccggacgga cgccagaacg ttagggatca ccgccccgct acgcatgtca ggcgatctca
     7321 gcggcactcc ggggattcgt ttgataagcc catttgcgga actggagatc acatctggcg
     7381 tcattgtcgc gcaacggcat attcatatgt ctccgctgga tgccctgatt tatggtgtta
     7441 cgcacggcga taaagtggcg gttgccattg aaggtgatga gcgacgactg atattcgaaa
     7501 acgtcactgt tcgcgtctct ccggatatgc gtcttgaaat gcatattgat accgatgaag
     7561 ccaacgccgc aggggctgat agccctgacg tttttgccac actggtgtca gtgacatgaa
     7621 taacatgctg atgcaacgta ttgttgacga agtggttttt cgccttaaac aacgtgcggg
     7681 aaaaacactc gtgctgactg tttttcaact gcgagacgcc agcgttcaag agagcgttca
     7741 tcagtatgcg tcattacaaa tccgatatgt cgatctgccg ctgctgcgac aacttgcgga
     7801 gaacgaaacg tcagatcgag cagcaatcca gatacacgaa gcgttagcct ggggaatgca
     7861 cattcaattg tcgttgcagc gtcatttcct gaacgccatt gaattgaaga ctctcgcccg
     7921 tctaccgcta agctggtgcg acgaacaagg gcaaccgatc tacctacatc atgatcgact
     7981 tttaagttat gtcgatatcg cacagttaag ctcgggaata ttggtgttac agcggaaatg
     8041 ttgtgtgacc gcactggctc gtgaagcggc aattacccga aatattcagt taatcaggca
     8101 ggagtaacct atgcatctgg ctcgagtcac tggcgcggta gtatccacgc aaaaatcacc
     8161 ttctctgatc gggaaaaaac tgctgctggt tcgccaggtc agtgctgacg gcgaactgcc
     8221 tctcacgcca ttaaccggcg acgaagttgc cgttgattcc gtcggcgcag gcgttggtga
     8281 gttggtactc ctcagcagtg gttccagcgc cagacatgtg ttttccggcc ctaacgaagc
     8341 catcgacctg gcagtggtcg gcattgtcga tacgctttcg cgctaaggac ctgaaagatg
     8401 gcaatttata cccgaacagg tgattcagga agtacgtctt tatttaccgg acaacgcgtc
     8461 agcaaaactc atctacgtgt cgaaacctat ggcacgctgg atgaactgaa cgcgacactc
     8521 agtctgtgtt attgcgccac cgccatcgaa agccatcgca tcttactgga agccattcaa
     8581 caacagatat tttggtttag cgccgaactc gccagtgaaa gcgaacagcc gtcagcgcag
     8641 cagcgttaca tcggtacgga agaaattgcc gcgctggaaa acgcgatcga tagcgcaatg
     8701 aacgccgtcc ccccggttca ctgcttcatt ctgcctggtc gatgtgaagc cgcaagccgc
     8761 atgcatttcg cccgcacggt ggctcgacgg gctgaaagac gtctggttga actgacaaca
     8821 gaaaccaccg tccggaatgt tttgctgcac tatatcaacc gactttccga ttgcctgtat
     8881 gccctcgccc gcgtggaaga caacgttgct catcaaaacc tgatgattca ggaaatcaca
     8941 aaacgttatc acgaagccaa ccacacacca gcattgaagg aacgcacaat gccgctcact
     9001 tttcaggatc ttcaccagct cattcgtagt gcggcaatgc gcgcagatga actgcatatt
     9061 cccgttgtca tcagcattgt cgatgccaac ggaacagaaa gtgttacctg gcggatgcct
     9121 gatgccctgt tggtcagcag tgaactggca ccgaaaaaag cctggaccgc cgtggcgatg
     9181 aaaacggcaa cccacaagct tgctgatacc gtgcaacccg gcgcgccgct ttatgggctc
     9241 gaaagccata tgcaaggcaa agtcgtcacc tttggtggcg gcttccccct ctggcgtgac
     9301 ggaaaattgc ttggcgggct tggcatcagc ggcggtagcg ttgaacaaga catggatatt
     9361 gcccaaagcg caatggcggc aattaacgtg ggagtaaacc aatgaataac tctgaattag
     9421 aaacactgat ccgcaatatt ctgcgtgagc agcttgtccc cgctacgcaa cgagcgcaaa
     9481 gaaacgctat tttccagact gttgatgaag cggtctgcgc agcacaccag gcctttttac
     9541 gctttcaaca gtgcccgctt aaaacccgta gcgccattat cagcgccatt cgcgaagaat
     9601 taatgccccg ccttaccgaa ctggcagaag aaagcgccaa agaaacgggg atgggaaata
     9661 aagaagataa gtacctaaaa aacaaagcgg cacttgataa cacgcctgga attgaagatt
     9721 taaccaccac agcactgacc ggtgatggag gcatggtttt gtttgaatac tctccgtttg
     9781 gggtgattgg ctcggtcacg ccaagcacca accccacgga aaccatcatt aacaacagca
     9841 tcagcatgct ggcagcaggt aatagcgtct acttcagtcc acacccggga gccaaaaacg
     9901 tttcgttaaa actgattacc ataatcgaag acatcgcgtt tcgctgtagt ggcattcaca
     9961 acctgatcgt taccgtatct gaaccaacct ttgccgcaac cgaacaaatg atggcgcacc
    10021 ctaaaatcag cgttctggcg attaccggtg gcccggggat cgtcgccatg ggaatgaaaa
    10081 gtggcaagaa agtgattggt gccggagcgg gtaacccacc ctgcattgtc gatgaaacgg
    10141 cggatattgt gaaagccgcc gaggacatca tcaacggcgc ttcatttgat tacaacctgc
    10201 cgtgtatcgc tgaaaaaagc ctgattgtcg tggtatcagt ggcagagtat ttaattcagc
    10261 agatgcaaag ctttggagca cttcttctta accatgaaca aacggagaaa ttgcgtgcaa
    10321 tttgcctgcc agacggtagc gccaacaaaa aactggttgg gaaaagccca tcagccttgc
    10381 tggaagcagc gggcctccct ttgcccgcaa aagcgccgcg cctactgatt gctgttgttg
    10441 acgccgatga tagttgggtt acgtgtgaac aactgatgcc aatgctgccc attgtgaagg
    10501 tcaacgattt tgacagcgct ctcgccctgg cattgaaagt ggaagacggc ttgcaccaca
    10561 cggcaattat gcactcgcag aacgtctcac ggctcaacct tgccgcgcgc gttatgcaaa
    10621 cctcaatctt tgtcaaaaac ggcccctcct gggcgggtat tggcgtcggc ggcgaaggct
    10681 ttacgacctt taccattgca acaccaaccg gagagggtac aacgtcagca cggacttttg
    10741 cccgctcacg acgctgcgtg ttaaccagcg gcttttccat tcgctagaga aacggaaatg
    10801 aaaacttttt cactgcagac gcgtttatac agcggaacgg gaagtctcaa catactgaac
    10861 cgtttcacga ataggcacat ctggattatt tgcgacgtct ttctggcaca ctcaccactc
    10921 attgacacgt tgcatcaggc gctgcccgac agtaatcgtc tcagtatttt tagcgaaata
    10981 acgcccgacc caactatcca gaccgtggtg aaaggcattg cgcaaatgca gactttacgc
    11041 ccggatgtgg ttatcggttt tggcggcggt tctgccctgg acgccgcaaa agcgattgtc
    11101 tggttcggtc gccagtgtgg cattgaaatt gaaacctgcg tagccattcc taccaccagc
    11161 ggtaccggtt ccgaagtgac cagtgcttgt gtcatcagcg atcctgagaa agggattaaa
    11221 tatccccttt tcgataatgc actttatccg gatatcgcga tccttgatcc atctttggtc
    11281 gtcagcgtgc caccggcgat aacggccaat accgggatgg atgttttaac ccatgcgctt
    11341 gaagcttatg tttctccccg cgccagcgac ttcacggatg cactggtcga aaaagccgtg
    11401 caaattgttt ttcaatacct gcccactgca gtgaagaaag gcgattgtct tgccacccgt
    11461 ggcaaaatgc ataatgcatc gacgcttgca ggaatagcct ttagccaggc cggactcggt
    11521 ttaaaccatg ccattgctca tcaactcggc ggacagtttc atctgccgca cggcctggcg
    11581 aatgcgctcc tgctcacggc ggttatccac tttaatgctg gcgatccgcg cgcagccaaa
    11641 cgctacgccc ggctggcgaa aacgtgccat ttatgcccgg acaacgccaa tgacaccgcc
    11701 agcctgaatg cacttattca acatattgag cagcttaaaa caacctgtac gctgccgact
    11761 cttaccaatg cgctgaaaga aaaaaaagca gaatggtcaa tacgcatacc agatatggtt
    11821 caggcggcac tggcggatgc aacgttgcgt accaacccgc gtgcggctga tgcctccgct
    11881 attgcagaac tgctcgagga gttgttatga acgtcgagtt aacctcctgc gacgaccaga
    11941 ccatccgcga gcgagtacag gcagcaggtg tcgtgggtgc tggcggcgca ggttttccaa
    12001 cccatattaa gctacaagcg cgtgttgaca cttttctggt gaatgcggcg gaatgcgaac
    12061 caatgttgaa agtggatcaa cagttaatgg tgcagcaagc cagccgcctg cttcgcggtg
    12121 ttcactatgc gatgaaagcc accggggcca gtagcggaat catcgcgctg aaagagaaat
    12181 atcaacgggc aataaacgca ctcacccccc tattaccacc ggatatccgc ctgcatattt
    12241 tgcccgacgt ttaccccgca ggagacgaag tgctgaccat ctggatggca actggtcgcc
    12301 gcgttccacc cgctgcatta cccgttaacg tcggtgtggt ggtcaataat gttcaaaccg
    12361 tacttaatat tgccagagcc gtcgagcaac agtatccagt gacgcgccgc acactcacta
    12421 ttaatggtgc agtggcgaaa ccaataacgg taaccgtccc catcggcatg tctttacgtg
    12481 aagtcctggc gctggcgggc ggcgcaacag tagatgctcc tggttttatc aatggcgggc
    12541 cgatgatggg aaatttgatc acatcgctcg acacccccgt cagcaaaacc actggcggct
    12601 tgctggtttt acccaactct cattcactga tccaacgtcg aatgcagaat gaccgcagcg
    12661 tacttaccgt cgcgaaaact gtttgtgagc aatgccgctt gtgtaccgat ctctgcccac
    12721 gtcatttagt cggtcacgaa ctggcacccc atttattagt acgtgcggtt aactaccaac
    12781 agctcgctac gccacaactg ttactgacgg cgctcacctg ttcggagtgc aacgtctgcg
    12841 ccagcgtcgc ctgtccggta ggaatttcac caatgcgcat aaaccgcctg cttaagcagg
    12901 aattacgcgc acaaaatctc cgctacgaag gtgccttaaa tcctgctgat cccatggcaa
    12961 attatcgact gattcccgta aaacggctgg taaccagact gggcctaatc ccgtggtatc
    13021 aggatgcgcc attgtctgaa caggtgcctc agcccgaaaa agtgacgtta ttgttacgcc
    13081 agcacatcgg tgccagcgca attgcgtgtg tacaaaaagg cgatcgcgtc gttcatgggc
    13141 aatgtgtagg tcaaattcca cacggcacat taggtgcccc cattcatgcc agtattgatg
    13201 gcatggtcag cgatgtgaca gaaaacgcga ttacgcttgt gagaggttaa cgatgtcgaa
    13261 agcggtaggt attttagaac ttagcagtat cgcgaaaggg atggaaaccg gcgacgccat
    13321 gctgaaaagc gcaaacgttg agttactggt cagcaaaacg ctatgccccg gaaaatttct
    13381 gctgatgctc ggtggcgatg tgggtgcggt gcaacaggcc atcaacagcg gtgcttccct
    13441 tgctggtgaa atgctggtcg acagtcttgt cctgccaaat attcatccca gtctcctttc
    13501 tgcaatcagc ggactgaacc atgttgaaca gcaccgggct gtcggcgtag tcgaaacctg
    13561 gagtgtcgcc gcctgcattg acgctgccga ttgtgctgtc aaagctgcga atgtcattct
    13621 cgttcgcgta catatggctt ttggcattgg tggtaaatgc tacatggtgg ttgctggcga
    13681 tatttcagat gttaataacg cggtctctgt cgccagcgag cgtgcggggg aaaaaggttt
    13741 gttggtctat cggtcagtga ttgcccgtcc acatgaagcc atgtggcgtc agatggtgga
    13801 gggataatga caactcagca cacaactgaa cggatgattc aggaatatgt ccctggtaag
    13861 caggtcacgc tggcacatgt catcgccaat cctgggaagg atctgtttaa gaaattgggt
    13921 ttaccagaca ccgtttctgc tattggcatt ctgacgatta cccccagcga agcatctatc
    13981 atcgcatgtg atatcgccac aaaatcaggc gctgtagaga tcggttttct tgatcgtttt
    14041 accggtgccg tagtgctgac tggtgatgtt tctgctattg aatatgcact tagccaggtt
    14101 acccgtacgc tgggtgaaat gatgcgcttt accgcctgcc ccattacgcg gacctgacgt
    14161 tatgcaacgt atgatgctta tcggccccag ccagtgcgga aaaacgtcac tcacgcaatg
    14221 tctgcgtggc gaaaatatcc attatcagaa aacgcaggca atcgtctggt ccccgaccac
    14281 cattgataca ccaggcgaat acctcgaaaa ccgcagcctc tacagtgcat tgttggtcag
    14341 tgcctgcgaa gcagacatta tcgcgctggt acttaatgcg gacgcaccgt ggtcaccttt
    14401 ttcgccaggc tttacctcgt caatgaatcg cccggtcatt ggagtagtaa ccaaagcaga
    14461 tttagccagc atggaacaaa tatctttggt gaaatgttgg ttacgggaag ctggagcgca
    14521 caacgtgtta gtaaccagtg cggttaataa caatggagtt acagaacttt ttgccttgct
    14581 gcatacagaa gaaggctgcc gctaatttcg ataggttctc agatgagaat gatgacggtg
    14641 atacacagat aagaatcaat gagcaatgag ggttgccggg caaccctcat tgaataaaac
    14701 aggaattact tcgcttcgcc gtttttcatt tcgcccatag ctttcagctt tttggagata
    14761 tcgcggcgtt ctttggagag ctcggcattt ttgatgatgt aatcgtcaac gcgatcttca
    14821 tagtcacctt tcatgctggc gatgatgccc tggattgctt caacgctcat ccctggcttg
    14881 atgtaatcgc tcaggttgtc cagcagcagg acgcgcttct ggttgtcacg gatctttttc
    14941 tcaacgtcct gaatttcacg ttgcagtttg ttcttacgac ggaacagacg aacaaattcc
    15001 agtacgtcct ggaatgaagg cttggtagtt tccattttta tacccctgat aatgtgagag
    15061 tcggattcgt ttaagcaacc gctattcgtt agggccaaca ttactcatag ctgccgcaga
    15121 tgacaatgct tttatccctt ttcactatca taccctttat ccttgctgaa tcgaagcagc
    15181 agcaagatga ttctgaagtt caggaattat ttgcgcaagg cccggcaaat cagattcgat
    15241 agcaactcaa gctgatgtgc cgtttccatg ttcagccaca cataaccata gattggtgtt
    15301 tccacaacct tcaggtcatg gtggttatcg agcaactgac gcagctcttc cacagcatcg
    15361 ttcaattttt ccgtattggc aaaaaccggc tgcggattac cttcgtacag cgcatgaaca
    15421 aggctcagca gtatttgctg catcatgtgc tgggtatcac gaagtttttg cgcgttcaat
    15481 aacacgaaat ggctggggcg cgtggcccag tatgcattga tttgcaactc cagcatacaa
    15541 accagattgc gattaatggt ctggatacct tcatatatcg attttggaat acgggtttct
    15601 ttgctggcgg gcgcaatcag cccacgcatt ttcacggcat cggtcaggag tttttgtaga
    15661 tggctttcca gacgtgggcg ttcgagtaag ttcggtgaga atgcagattg atagacccga
    15721 ttatactcgg tcagactttt cgccagttga atgcgccagt ggatgaacgc ccgttgtggc
    15781 cagataccgg taaacaacat tgccagtaaa gagccgagga tcacatcgcc gcttcgccat
    15841 aacgccgtat caatttcacc tgtcggggaa cccacaacaa ttgccagcgt caccccaatc
    15901 aataaacctt gatatggttt cttgcccagc gccagccaac cgcaaaggaa catggccgcc
    15961 gcgcaccaga ctaacatcag cggtaacgag attaactcca gttgcagagc gataagacct
    16021 aaaatcgacc ccaacaccgt accgccaata cgttcgaagg cacgagggac aacgttcccc
    16081 cagaacgaga ttggccccat aatcaccacc atggtgacca gcggccaggt gctttccggg
    16141 atagtaaaca ggcggataat gagaaaagtg agcaggaatg ccagcgcgac ccgagtacca
    16201 tgcacaatgc ggtaatggcg gtatacccgg atttcaaacg ggcttaatga cttatcggca
    16261 cgcacgcaga aactccgtaa tgaagattaa aagcgctcat aactcatctg tcggccagaa
    16321 ggcgcgcctc tgaattatcc gacggacaaa ccgggaacag tgtaatttcc cggcaggagc
    16381 gaaaaaattg aaaggcgcat cttactcttt tcgctttcaa aaaaagagtg gtcttcgcgt
    16441 caacacaccg ccctgagatg aattagtgat gttttggctg caccgcaaca tacatttcga
    16501 tatcccagta cccatcttcc gcgccattgt tcaagtaaac ctcgaagcag ggctttggta
    16561 acatttcata agcactgtcc tgcaagaggc tattaaagaa ctgataccag ggtttagcaa
    16621 aatcatcacc gactacacga gccaccgcca ccgcatactg accacctgaa atttctgtca
    16681 gaatgacgcc ctcactgttt tcggggagcg taaagttatt cggtaccgtc acgacggtgt
    16741 cgcagcgtaa tttttcggcg ggtgtttcat ctggattgtc gtaatagaca gcaacccact
    16801 ccttcggcac aatatttttg ctatctatcc acatcatcaa ctgctcaaaa cctttcttta
    16861 ccgtctgctc ccacgggcca acaagatgga aacctgcaac ggtacgtttc tcttcctgct
    16921 taatctcgta gttcatgacg cctccattaa cactgttttt atatacagta tagttgcaat
    16981 attaaaacca caaggaatga gtgttgatta tgcgagcaga ctcgcactcc tgccagtctg
    17041 ctgcaaaaga aaggtcaggc cttatggtgg aaataatcac tcaggcggga aaacatgccg
    17101 ccttccccga cagattccag ggtaaccagc ggccagtgcg ccacctgttt atcacggtcg
    17161 taaagttcaa tttcccctac ccgctgatgg gcgctaattg gcgcggtgag ctctttacca
    17221 tcaagggtat atttggcttt gatatgtgga atttcggctt tcggtagcac catccagaac
    17281 tcttgttccg ttcccaggtc gatattttct ttatcgccat accagatgcg ttccgttccg
    17341 acctttttcc cacggtgcaa aatttgcacc gtagtaaagt tttgttgccc ccaacgcagt
    17401 aattttcttg cctcttcctc acgacctttt gcactgtcag cccccattac cactgcaatg
    17461 agacgacgct gcccatctac agccgaagca atgagattaa acccggcacc agaagtatga
    17521 cccgttttca ggccgtcaac attcatggtt ttatcccaca acaacccgtt acggttttgc
    17581 tgggtgatac cgttccaggt gaggcttttc tcactgtaca tatgataaaa ctcgggctcg
    17641 ccgtggatga tagcgcgaga aagcacagct aaatcataag ccgagctatg ctggccaggt
    17701 gcatccagac catgcactgt ttcaaaatgc gtatccttga gatgcagctt ctcggcatag
    17761 ttgttcatca ttgcaacaaa ctgccgttgc ccaccggcaa tatagtcagc cagtgcaaca
    17821 caagcatcat ttccggaatc cacaattaaa ccacggctta aatcacgtac cgatacgcga
    17881 tcgccctctt tcaaaaacat cagtgaagaa ccgacaaaca ctggattatc tttcgcccac
    17941 gcatcgcgcc ctacggtgac aatatcgtct ggcgtaattc gatgactatc gatagcgcga
    18001 tccacgacat aacccgtcat cagctttgtc aggctggcgg gattgcgctg ttgatgctca
    18061 ttacccgcgg tgaggatctg tccggtggtg taatccatca atacccagga cccggcatga
    18121 atctctggag gctgaggtga aaaaggaatg ttttccgccg caaaaccaga cgataagtta
    18181 aaaacgaaca aagaagcagc aataataaga cggcgtttca acagcaaacc ctcaggagtt
    18241 tcaaatagct gttcttttta cggaaatact tatgaactgg ctggaataaa gtgcaagaaa
    18301 atgtgactaa cctctcattt ttatctgaca tgatctgttg ccactcgctg ccaaattgtg
    18361 gcgctaaagc tgattagcac ggtgatattt gatactctgg cagacagcag agataacgga
    18421 tttaacctaa tgatgaatga cggtaagcaa caatctacct ttttgtttca cgattacgaa
    18481 acctttggca cgcaccccgc gttagatcgc cctgcacagt tcgcagccat tcgcaccgat
    18541 agcaaattca atgtcatcgg cgaacccgaa gtcttttact gcaagcccgc ggatgactat
    18601 ttaccccagc ctggagcagt attaattacc ggtattaccc cgcaggaggc acgggcgaaa
    18661 ggagaaaacg aagccgcgtt tgccgcccgt attcactcgc tttttaccgt accgaagacc
    18721 tgtattctgg gctacaacaa tgtgcgtttc gacgacgaag tcacacgcaa cgttttttat
    18781 cgtaatttct acgatcctta cgcctggagc tggcagcatg ataactcgcg ctgggattta
    18841 ctggatgtta tgcgtgcctg ttatgccctg cgcccggaag gaataaactg gcctgaaaat
    18901 gatgacggtc taccgagctt tcgccttgag catttaacca aagcgaatgg tattgaacat
    18961 agcaacgccc acgatgcgat ggctgatgtg tacgccacta ttgcgatggc gaaactggta
    19021 aaaacgcgtc agccacgcct gtttgattat ctctttaccc atcgtaataa acacaaactg
    19081 atggcgttga ttgatgttcc gcagatgaaa cccttggttc acgtttccgg aatgtttggg
    19141 gcatggcgcg gcaataccag ctgggtggca ccgctggcgt ggcatcctga aaatcgcaat
    19201 gccgtaatta tggtggattt ggcaggagac atttcgccat tactggagct ggatagcgac
    19261 acattgcgcg agcgtttata taccgcaaaa gccgatcttg gcgataacgc cgccgttccg
    19321 gttaagctgg tgcatatcaa taaatgtccg gtactggccc aggcgaatac gctacgcccg
    19381 gaagatgccg accgactggg aattaatcgt cagcattgcc tcgataacct aaaaattctg
    19441 cgtgaaaatc cgcaagtgcg tgaaaaagtg gtggcgatat tcgcggaagc cgaaccgttt
    19501 acgccttcag ataacgtgga tgcacagctt tataatggct ttttcagtga cgccgatcgt
    19561 gcagcaatga aaattgtgct ggaaaccgag ccgcgtaatt taccggcact ggatatcact
    19621 tttgttgata aacgaattga aaaactgttg ttcaattatc gagcgcgcaa cttcccgggg
    19681 acgctggatt atgccgagca gcaacgctgg ctggagcacc gccgccaggt cttcacgcca
    19741 gagtttttgc agggctacgc tgatgaattg cagatgctgg cacaacagta tgctgatgac
    19801 aaagagaaag tggcgctgtt aaaagcgctt tggcagtacg cggaagagat cgtctgaaac
    19861 aaaaaagccg gagcgacaaa ctccggcttt ttgagattta ccggatgatt atgtcatccg
    19921 gtttgtaaga ttacgctacg tcttcgtact gcggaaccgg gttacggaaa ctcttggtta
    19981 cgcaagccag gtaaatcaga ccgatagcag cccagatcag acccagaacc attgagcttt
    20041 cttccaggtt aacccacagc gcaccaacgg tcagcgcacc acacatcggc aggaacagat
    20101 actggaagtg atctttcagc gtcttgttac gcttctcacg gatccagaac tgcgagatga
    20161 ccgacaggtt aacgaaggtg aacgccacca acgcaccaaa gttaatcagc gccgtagcca
    20221 ttaccaggtc gaagttgatt gccagcaggg caatcgcgcc aaccaggatg atgttcatcg
    20281 ccggagtacg ccatttcggg tgtacataac cgaagaagct tttcgggaac acgccgtcac
    20341 gacccattac gtacatcaga cgcgctacgc ctgcgtgcgc cgccataccg gacgccagta
    20401 cggtaatggt ggagaagatc agcgcgccaa cctggaacgc tttacccgca acgtacagca
    20461 tgatttcagg ctgtgacgca tccggatctt taaagcgaga gatatccggg aagtacagct
    20521 gcaggaagta agttgcaaag atgaagatca tgccgccgat cagcgcggtc aggaaaatcg
    20581 cacgcgggat cacgcgctct gcatctttgg tttcttccga caggttgctg atgccgtcaa
    20641 aaccggtaaa ggagaagcac aggattgtcg ccccggtaat catcgggata acatgcgcat
    20701 cgccagacca gaacggacga gtgctcgcca gcgtaccagc gccttcacct tcaaatacgc
    20761 cataaacaac catgcccaga atcaccgcga tcagcactac ctgcaacacg acgattacag
    20821 tgttgaagtt cgctacggat ttcagactac gcaggttaaa ggcggtcata aaggccacca
    20881 gcgccaccac aaacatccac gatggaatgg aaggcaccag agcttcaaaa tagattttcg
    20941 ccagcagaat gttgatcatc ggcgcgaaca gatagtcgag cagagaagac caacccacca
    21001 taaagccgac agtcgggcta atggatttct gggcgtaagt gtatgcagag ccagcagaag
    21061 gatagcggcg aaccagcttc ccgtagctca gagccgtaaa caggatcgca atcaatgcga
    21121 acgcataggc tgtcggcaca tgaccatccg tgaggcctga aacgatacca aaagtatcaa
    21181 acagcgtcat cggctgcata taggctagac ccatcattac aaccggaact aacgtaagcg
    21241 ttttacgcaa ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg gtcattctcc
    21301 tttacggtga taaccgtcgc gtaagcaaaa aattgcccca tttttttgga ttcctcagcg
    21361 acaacaactg tcgattttta gtaaatatct atccggtacg aagcccggcc tcttggtatg
    21421 aatgattggt ttgaagcaaa aaataaccga cgctaattaa acgtcggttt ttagtcattt
    21481 tttgacagcg gcgcattgtg cctaaatggg ggggaaatga caagagaatg agaggcttgt
    21541 cataataatt tttctttaaa tggctgattt tccgtcatca gattttgcat aaacaccggc
    21601 gatcgcacta tttgctaaaa tttcgtcccg ccaccaacca ctggcaagcc ctgcggtctg
    21661 ttcattccag ccaatccaga cgggttcaaa gagagtttgg gcgaccactt tcttctcgat
    21721 taacgcgcca ctatcgagaa aacgttgcgc cagataccgt ggtaaatagc cgcagccaag
    21781 gccgctaatt tgcagctcca gcttggtttt aaaatcgaaa acggtaatgg cttcttgttc
    21841 gtcgagaagt tgtgaggccg tagaagcgcc tgcctgcgca gtgtccccca ccacaatcgc
    21901 ccgatatcgt ttaatgatac gacggtttaa cggttcttct tcctgggcta acggatgatg
    21961 aggcgcgacc gcaaagactt gttcaagatc gcccaaccgc gagaaaccaa actcactgga
    22021 agatggtggc tcatgcatcg cgccaacaat aatatccgct cgcccctgag tcagtgcatc
    22081 ccaggaacca ccgagtacgc cattgataaa cttcaggcgc gtcacgctat gatgttgata
    22141 aaaagcctca atgagaggcg caagcagaga aaaaggaaac gtgtcgtcca cgccaatcac
    22201 cagctcattt tcccagcctt catgcagttt gatagcctgc ttttccagtt ctcgcacggt
    22261 atgcagaact tcccgccctt tctctaataa catttttccg gtgcgggtga atttagcgcg
    22321 gtgaccgcta cgatcaagca attgaatatt aaggtcgctt tccagcttgt gaacggtata
    22381 actcagagcg gacggcgtct tgtagagttt agccgacgcc gccgcaaaac tgccttcttt
    22441 ttctaaggca tcgaggatca tcaacacatc cagcagtggt ttcatacacg cccccttgca
    22501 gtgtatgcgg cgtcctgctg gcgtgatcgt ttactccagc ggcattacca gcggatcggg
    22561 atactggtat tcaaatccca gttcattaca aatccgactg ccatcaataa tcttaccttt
    22621 gccgctgtcc agactatttc tgaactgcgg aggttccagc cccagtaaac gggccatctg
    22681 cggatagaaa acattacgcg cagggtgagc tggcgcacat atattataga tgtgtccgcc
    22741 tttaggtgcc tgtaacaaca gagtgatagc accaataaca tcttctaaat ggactaaatt
    22801 aacaccatgt tcaccatcag gggcggtttt tccggcaaag aagcgtccgg gatgacgtcc
    22861 cggtccgacc agccccgcaa gacgcagaat atcgaccgaa gtaccgggta aattgtgcag
    22921 ccagtcttcg agttcttcta atactcgtcc actgttggtc accggattac gcggggtggt
    22981 ttctttcacc gtgccttgcg cgtcgccata gacagatgtt gagctggtaa aaataattcg
    23041 gggaatacga tgggccagcg cgctatccac taactcttgt accgcttgta aatagaactc
    23101 atcgccgggg ccgctacgac gtgccggaag cgtaatgacc agcgcgtcgg catccatcag
    23161 ggcatccaga tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca gatagctatc
    23221 aatgccactc attcgggccg cttcgacacc atcttgtgtg gttttactcc cggtgacttg
    23281 ccagcctcgc gctgaaagtg acatcgccag cggcatgcct aaccacccta acccgacaat
    23341 tgcgaccttt ttcatccgtt atctcctgat acaccttaga tctataaggc tacgctagcg
    23401 tatcaaaact gacaattcat tctatgaatg aatctgttca ataatgataa cgacatgctg
    23461 caatgagtag tgaatcgtcg gtaaccgcgt ataccagacg gtgctcctct gtaatgcgtc
    23521 gggaccagaa acctgacaaa ttatgtttca ggggttctgg tttcccctta ccttcaaatg
    23581 gtgttctgcg ggtatctttg ataagttcat tgatcttttt aacaattcgc ttatctgttt
    23641 cctgccagta cagataatca tcccatgatt cctcagacca gattagtttc actcaatgat
    23701 gtccttttcc gttcctttgc cagatttcag gctatcgatt gagtccatca atctccgggc
    23761 gttagcgggg gagcgcagta gataagccgt ctcctccagg gagttgtatt cttcgagtga
    23821 catcagaaca caagcctctc cattctgacg agtaataagg atcggggcat gatcttcaac
    23881 ggctttcatc attgttgccg acaaattctg acgcgcttcg ctgtagctaa ttgtacgcat
    23941 gtcaatctcc tcttttgtac agttcattgt acaatgatgg gcgttaatta actatttatt
    24001 aattagtttg tagatcaagg tattgtcagt gagacgaaaa tccaggcttc gctatttttg
    24061 gtgccgtcag cttagaggac agtcctctta gccccctcct ttccccgctc attcattaaa
    24121 caaatccatt gccataaaat atataaaaaa gcccttgctt tctaacgtga aagtggttta
    24181 ggttaaaaga catcagttga ataaacattc acagagactt ttatgacacg cgttcaattt
    24241 aaacaccacc atcatcacca tcatcctgac tagtctttca ggcgatgtgt gctggaagac
    24301 attcagatct tccagtggtg catgaacgca tgagaaagcc cccggaagat caccttccgg
    24361 gggctttttt attgcgcggt tgataacggt tcagacaggt ttaaagagga ataagaaaat
    24421 gacagacaac actcgtttac gcatagctat gcagaaatcc ggccgtttaa gtgatgactc
    24481 acgcgaattg ctggcgcgct gtggcattaa aatcaatctt cacacccagc gcctgatcgc
    24541 gatggcagaa aacatgccga ttgatattct gcgcgtgcgt gacgacgaca ttcccggtct
    24601 ggtaatggac ggcgtggtag accttgggat tatcggcgaa aacgtgctgg aagaagagct
    24661 gcttaaccgc cgagcccagg gtgaagatcc gcgctacttt accctgcgtc gtctggattt
    24721 cggcggctgc cgcctttcgc tggcaacgcc ggttgatgaa gcctgggatg gcccgctctc
    24781 cttaaacggt aaacgtatcg ccacctctta tcctcacctg ctcaagcgtt atctcgacca
    24841 gaaaggcatc tcttttaaat cctgcttact gaacggttct gttgaagtcg ccccgcgtgc
    24901 cggactggcg gatgcgattt gcgatctggt ttccaccggt gccacgctgg aagcaaacgg
    24961 cctgcgcgaa gtcgaagtta tctatcgctc gaaagcttgc ctgatacaac gcgatggcga
    25021 aatggaagaa tccaaacagc agctgatcga caaactgctg acccgtattc agggcgtgat
    25081 ccaggcgcgc gaatcaaaat acatcatgat gcacgcaccg accgaacgcc tggatgaagt
    25141 catcgccctg ctgccaggtg ccgaacgccc aactattctg ccgctggcag gtgatcaaca
    25201 gcgcgtagcg atgcacatgg tgagtagcga aaccctgttc tgggaaacga tggaaaaact
    25261 gaaagcgctg ggtgccagtt caatcctggt cctgccgatt gaaaaaatga tggagtgatc
    25321 gccatgagct ttaacacaat cattgactgg aatagctgta ctgcggagca acaacgccag
    25381 ctgttaatgc gcccggcgat ctccgcttct gaaagcatta cccgcaccgt taacgatatc
    25441 ctcgataacg tgaaagcacg cggcgatgag gccctgcgtg aatacagcgc gaaatttgat
    25501 aaaaccacgg ttaccgcgct gaaggtgtct gctgaggaga tcgccgccgc cagcgaacgc
    25561 ctgagcgacg agctaaaaca ggcgatggcg gtggcagtaa agaatattga aaccttccac
    25621 actgcgcaaa aactgccgcc ggtagatgtc gaaacgcagc caggcgtgcg ttgccagcaa
    25681 gtcacgcgcc cggtcgcttc agttgggttg tatattcctg gcggctccgc cccgctcttc
    25741 tcaacggtat taatgctggc aactccggcg cgtattgcgg gctgtaaaaa agtggtgctg
    25801 tgctcaccgc cgccgattgc cgacgaaatt ctttacgcgg cgcagctgtg cggtgtgcag
    25861 gatgtgttta acgtcggcgg cgcacaggcc attgccgcac tggcgtttgg tacggaatct
    25921 gtgccgaaag ttgacaaaat cttcgggcca ggtaacgcct ttgtcaccga ggcaaaacgt
    25981 caggtgagcc agcgtctgga cggtgcggcg atcgatatgc ccgcaggccc gtcggaagtg
    26041 ctggtgattg ctgacagcgg cgctacgccg gatttcgtgg cttctgattt gctttctcag
    26101 gctgaacacg gcccggactc gcaggtgatt ttactgacgc ccgatgctga tatggcgcgt
    26161 cgcgttgccg aggctgtcga acgccaactg gcagaactgc cgcgtgccga aaccgcccgc
    26221 caggcactga acgccagccg cctgatcgtg actaaagatt tagcgcagtg cgtagagatc
    26281 tccaaccagt acggcccgga gcacctgatc atccagaccc gcaacgcccg cgatctggtc
    26341 gatggcatca ccagcgccgg ttcggtattt cttggtgact ggtcacccga atcggcgggt
    26401 gattacgcct ccggcaccaa ccacgtactg ccgacttacg gttacaccgc cacctgttcc
    26461 agcctcgggc tggcagattt ccagaagcgt atgaccgtgc aggaactgtc gaaagaaggt
    26521 ttctccgcgc tggcttcgac catagaaaca ctggccgccg ccgagcgcct gaccgcccat
    26581 aaaaatgccg ttactttgcg tgttaacgcc cttaaggagc aagcatgagc accgtgacta
    26641 ttaccgattt agcgcgtgaa aacgtccgca acctgacgcc gtatcagtcg gcgcgtcgtc
    26701 tgggcggtaa cggcgacgtc tggctgaacg ccaacgaata ccccacagcc gtggagtttc
    26761 agctcactca acaaacgctc aaccgctatc cggaatgtca gccaaaagcg gtgattgaaa
    26821 attacgcgca atatgcaggc gtaaaaccgg aacaggtgct ggtcagccgt ggcgcggacg
    26881 aaggtattga actgctgatt cgcgcttttt gcgaaccggg taaagacgcc atcctctact
    26941 gcccgccaac gtacggcatg tacagcgtca gcgctgaaac cattggcgtc gagtgccgca
    27001 cagtgccgac gctggacaac tggcaactgg acttacaggg catttccgac aagctggacg
    27061 gcgtaaaagt ggtctatgtt tgcagcccca acaacccgac cgggcaactg atcaacccgc
    27121 aggattttcg cactctgctg gagttaaccc gcggtaaagc gattgtggtt gccgatgaag
    27181 cctatatcga gttttgcccg caggcatcgc tggctggctg gctggcggaa tatccgcacc
    27241 tggctatttt gcgcacactg tcgaaagctt ttgctctggc gggccttcgt tgcggattta
    27301 cgctggcaaa cgaagaagtc atcaaccttc tgatgaaagt gatcgccccc tacccgctct
    27361 cgacgccggt cgccgacatt gcggcccagg cgttaagccc gcaggggatc gtcgccatgc
    27421 gcgaacgggt ggcgcaaatt attgcagaac gcgaatatct gattgccgca ttgaaagaaa
    27481 tctcctgcgt ggagcaggtt tttgactctg aaaccaacta cattctggcg cgctttaaag
    27541 cctccagcgc agtgtttaaa tctttgtggg atcagggcat tatcttacgt gatcagaata
    27601 aacaaccctc tttaagcggc tgcctgcgaa ttaccgtcgg aacccgtgaa gaaagccagc
    27661 gcgtcattga cgccttacgt gcggagcaag tttgatgagt cagaagtatc tttttatcga
    27721 tcgcgatgga accctgatta gcgaaccgcc gagtgatttt caggtggacc gttttgacaa
    27781 actcgccttt gaaccgggcg tgatcccgga gctgctgaag ctgcaaaaag cgggatataa
    27841 gctggtgatg atcaccaatc aggatggtct gggaacacaa agtttcccgc aggcggattt
    27901 tgatggcccg cacaacctga tgatgcagat cttcacctcg caaggcgtgc agtttgatga
    27961 agtgctgatt tgcccgcacc tgcccgccga tgagtgcgac tgccgtaaac cgaaagtaaa
    28021 actggtggag cgttatctcg ctgagcaagc gatggatctc gccaacagtt atgtgattgg
    28081 cgatcgcgcg accgatattc aactggcgga aaacatgggt attaatggtt tacgctacga
    28141 ccgcgaaacc ctgaactggc cgatgattgg cgagcaactc actaaacgag accgttacgc
    28201 ccatgtagtg cgcaacacca aagagacgca gattgacgtt caggtatggc tggatcgcga
    28261 aggtggcagc aagattaaca ccggcgttgg cttctttgat catatgctgg atcagatcgc
    28321 cactcacggc ggtttccgca tggaaatcaa cgtcaaaggc gacctctata tcgacgatca
    28381 ccacaccgtc gaagataccg gcctggcgct gggtgaagcg ttaaaaattg ccctcggcga
    28441 taaacgcggt atttgccgct ttggttttgt gctgccgatg gacgaatgcc ttgcccgctg
    28501 tgcactggat atctctggtc gcccacacct ggaatataaa gctgaattta cctaccagcg
    28561 cgtcggcgat ctcagcaccg agatgatcga gcacttcttc cgttcgctct cttacaccat
    28621 gggcgtgacg ttgcacctga aaaccaaagg taaaaacgat catcaccgtg tagagagtct
    28681 ctttaaagcc tttggtcgca ccctgcgcca ggccatccgc gtggaaggcg acaccctgcc
    28741 ctcgtcgaaa ggagtgctgt aatgaacgtg gtgatccttg ataccggctg cgccaatttg
    28801 aactcggtga agtcagccat tgcgcgtcac ggttatgaac ccaaagtcag ccgtgacccg
    28861 gacgtcgtgt tgctggccga taaactattt ttacccggcg taggcaccgc acaagcagcg
    28921 atggatcagg tgcgtgagcg cgagctgttt gatctcatca aagcctgtac ccaaccggtg
    28981 ctgggcatct gcttagggat gcaactgctg gcgcggcgca gcgaagagag caacggcgtc
    29041 gatttgctgg gcattatcga cgaagacgtg ccgaaaatga ccgactttgg tctgcccctg
    29101 ccgcatatgg gctggaaccg cgtttacccg caggcaggca accgcctgtt tcaggggatt
    29161 gaagacggcg cgtactttta ctttgttcac agctacgcaa tgccagtcaa tccgtggacc
    29221 atcgcccagt gtaattacgg cgaaccgttc accgcggcgg tacaaaaaga taacttctac
    29281 ggcgtgcagt tccacccgga gcgttctggt gccgctggcg ctaagctgct gaaaaacttc
    29341 ctggagatgt gatgattatt ccggcattag atttaatcga cggcaccgtg gtgcgtctcc
    29401 atcagggaga ttatggcaaa caacgcgatt acggtaacga cccgctgcca cgcttacagg
    29461 attacgccgc gcagggtgcc gaagtgttgc atctggtgga tctgaccggg gcaaaagatc
    29521 cggctagacg tcaaatcccg ctgattaaaa ccctggtggc ggacgttaac gttccggtgc
    29581 aggttggcgg cggcgtgcgt agcgaagaag acgtggcggc gttactggaa gcgggcgttg
    29641 cgcgcgtggt ggttggctcc accgcggtga aatcaccaga aatggtgaaa ggctggtttg
    29701 aacgtttcgg tgccgatgcc ttagtgctgg cgctggatgt ccgtattgac gagcaaggca
    29761 acaagcaggt ggcggtgagc ggctggcaag agaactcggg cgtttcactg gaacaactgg
    29821 tggaaaccta tcttcccgtc ggcctgaaac atgtgctatg taccgatatc tcgcgcgacg
    29881 gcacgctggc aggttctaac gtctctttat atgaagaagt gtgcgccaga tacccgcagg
    29941 tggcatttca gtcctccggc ggcattggcg acattaacga tgttgctgcc ctgcgaggta
    30001 ctggcgtgcg cggcgtaata gttggtcggg cattactgga aggtaaattc accgtgaagg
    30061 aggctatcgc atgctggcaa aacgcataat tccatgtctc gacgttcgtg atggtcaggt
    30121 ggtgaaaggc gtacagtttc gcaaccatga aatcattggc gatatcgtgc cgctggcaaa
    30181 acgttacgcc gaagaaggtg cagacgaact ggtgttctac gatatcaccg cttccagcga
    30241 tggccgtgtg gtagataaaa gctgggtatc tcgcgtggcg gaagtaatcg acattccgtt
    30301 ttgtgtggcg ggtgggatta agtctctgga agatgccgcg aaaattcttt cctttggcgc
    30361 ggataaaatc tccattaact cccctgcact ggcagacccg acgttaatta ctcgcctggc
    30421 ggatcgcttt ggcgtgcagt gtattgtggt cggcattgat acctggtacg acgccgaaac
    30481 cggtaaatat catgtgaatc aatataccgg cgatgaaagc cgcacccgcg tcactcagtg
    30541 ggaaacactc gactgggtgc aggaagtgca aaaacgcggt gccggagaaa tcgtcctcaa
    30601 catgatgaat caggacggcg tgcgtaacgg ttacgacctc gaacagctga aaaaagtgcg
    30661 tgaagtttgc cacgtcccgc tgattgcctc cggtggcgcg ggcaccatgg aacacttcct
    30721 ggaagccttc cgcgatgccg acgttgacgg tgcgctggct gcttccgtat tccacaaaca
    30781 aataatcaat attggtgaat taaaagcgta cctggcaaca cagggcgtgg agatcaggat
    30841 atgttaacag aacaacaacg tcgcgaactg gactgggaaa aaaccgacgg actgatgccg
    30901 gtgattgtgc aacatgcggt atccggcgaa gtgctaatgc tgggctatat gaacccggaa
    30961 gctctggaca aaaccctcga aagcggcaaa gtcaccttct tctcgcgcac taaacagcga
    31021 ctgtggacca aaggcgaaac gtcgggcaat tttctcaatg tggtgagtat tgccccggac
    31081 tgcgacaacg acacgttact ggtgctggcg aatcccatcg ggccaacctg ccacaaaggc
    31141 accagcagct gcttcggcga caccgctcac cagtggctgt tcctgtatca actggaacaa
    31201 ctgctcgccg agcgcaaatc tgccgatccg gaaacctcct acaccgccaa actgtatgcc
    31261 agcggcacca aacgcattgc gcagaaagtg ggcgaagaag gcgtggaaac cgcactggca
    31321 gcaacggtac atgaccgctt tgaactgacc aacgaggcat ctgatttgat gtatcacctg
    31381 ctggtgctgt tgcaggatca ggatctggat ttaacgacgg taattgagaa cctgcgtaaa
    31441 cggcatcagt gagttgcggg tgatcagatg cgatgttgtt gtcgcatccg gcaaaaaaaa
    31501 cgggcaaggt gtcaccaccc tgcccgtttt ctttaaaacc aaaagattac ttcgcgttgt
    31561 aattacgcag agcgttacgc cccagcacaa tccccgcgcc aaccatgccg cccagcagca
    31621 ctgccagaat caaggtaatt gcctttttcg ggctatcgcg acgaataggt aacgtcggtt
    31681 tcatcacata gcggtaagca tgaatatcaa gatcatcaac ctttaagttt tcaatatcca
    31741 acaggttttg acgagtctga tagtagtttg gcgagaacac caacggacga gtcgcctcat
    31801 gcttaatcat cgactccagc gcttcgctcc ccaaaaggaa caacgtatct tgtgtcacat
    31861 cttcagtctg ctgaacctgt ggctttgtca cctgcgcctg attcgcatac tgcaacgctt
    31921 cctgaatctg acggatacgc agatctttct gctcttgcgc gactacttcc tgagttctta
    31981 aagagtcctg caagtttttc atacgcagcg cgatgttgtc tttcaggtct ttttccagtt
    32041 cctcattaac ctggtcgtca acttgctgga tatacttcgc cagctgcttc tgagcacctt
    32101 caggtgtttc accaacataa gacaccgcta aaggtaatga ctgattttta acggtaggct
    32161 caatggtcag cttttctgct tcttcctgat tatccagcgt ttccgctaat gctgaaaatg
    32221 cagtgctgta acgtccgata agtgaagcct gaatgtcgga gactttcggt acagccgatc
    32281 cgtagatcac attcatcgcg ttggtataac cagcgatttg ccccacatca ggttgagtaa
    32341 cgatagctgt ggaagtccac ttttctttgg caacggccag ataccctata gccagggcaa
    32401 tagcaacaat gacagaaatg atgatagtca tcttgccgcg ccacaactgc accagtaaat
    32461 caatcaaatc aatctgttcc gggtcatggt tttgcccaga aacattatta ttttctactc
    32521 tcatcattac cctaacggac gaaataattt taagtcgcca tagtttagcg ataatcgcca
    32581 tgaatgctat aggataaatg ataaaaagaa tgaattatta caaagggaac atgagagaat
    32641 cagggcgaaa atcgccctga taacaggatg ttagtcactg ccaaagagat cgcgggtgta
    32701 gactttgtct gccacatcct taagctcttc tgccatacgg ttggaaataa taacgtcggc
    32761 ttgttgtttg aaggtggcga gatcacgttc cagtcgagag ttgaagaatg aatcttcttt
    32821 catcaccggc tcgtagatga tcacttcaac accttttgcc ttgatacgtt tcataatccc
    32881 ctgaatggaa gacgcgcgga agttatctga cccgctcttc ataatcagac gataaatacc
    32941 caccacttgc ggtttgcgtg acaaaatggc atcggcaata aaatctttac gcgtgcggtt
    33001 tgcatcgaca attgccgaga tcaggttatt cggcacagac tggtagttcg ccagtaactg
    33061 cttggtatct ttcggtagac aataaccacc atagccaaac gacggattgt tgtaatggtt
    33121 gccaatacgc ggatcgagac aaacaccttc gataatctga cgagtattca gacctaaact
    33181 ttctgcatag ctatccagct cgttaaagta tgctacgcgc atcgccagat aggtattagc
    33241 aaacagttta atcgcttctg cttcagtgga gtcggtaaac agggtcggga tattttgctt
    33301 aatcgcgcct tcctgtaata acgccgcaaa acgttctgcg cgttctgaac gctcaccgat
    33361 gacaatacgt gacggatgaa ggttatcgta aagagcttta ccttcacgga gaaattccgg
    33421 agagaaaata atattttcag tacgatattt cttatgcatc gcttcggtaa aaccaacggg
    33481 aaccgttgat ttgatgacca taaccgcata aggatttatc tcaactacgt c
//
LOCUS       DAZGHF010000042        33357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGHF010000042 DAZGHF010000000
VERSION     DAZGHF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.042"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(30..2078)
                     /gene="prc"
                     /locus_tag="ACHK3A_002088"
     CDS             complement(30..2078)
                     /gene="prc"
                     /locus_tag="ACHK3A_002088"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO8740168.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQTFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(2098..2796)
                     /gene="proQ"
                     /locus_tag="ACHK3A_002089"
     CDS             complement(2098..2796)
                     /gene="proQ"
                     /locus_tag="ACHK3A_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO8740169.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(2893..3390)
                     /gene="msrC"
                     /locus_tag="ACHK3A_002090"
     CDS             complement(2893..3390)
                     /gene="msrC"
                     /locus_tag="ACHK3A_002090"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO8740170.1"
                     /translation="MNKIEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            3520..4803
                     /gene="yebS"
                     /locus_tag="ACHK3A_002091"
     CDS             3520..4803
                     /gene="yebS"
                     /locus_tag="ACHK3A_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO8740171.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPTTRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            4772..7405
                     /gene="yebT"
                     /locus_tag="ACHK3A_002092"
     CDS             4772..7405
                     /gene="yebT"
                     /locus_tag="ACHK3A_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO8740172.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            7485..8924
                     /gene="rsmF"
                     /locus_tag="ACHK3A_002093"
     CDS             7485..8924
                     /gene="rsmF"
                     /locus_tag="ACHK3A_002093"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO8740173.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            9042..9278
                     /gene="yebV"
                     /locus_tag="ACHK3A_002094"
     CDS             9042..9278
                     /gene="yebV"
                     /locus_tag="ACHK3A_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO8740174.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            9383..9574
                     /gene="yebW"
                     /locus_tag="ACHK3A_002095"
     CDS             9383..9574
                     /gene="yebW"
                     /locus_tag="ACHK3A_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO8740175.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(9575..10231)
                     /gene="pphA"
                     /locus_tag="ACHK3A_002096"
     CDS             complement(9575..10231)
                     /gene="pphA"
                     /locus_tag="ACHK3A_002096"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO8740176.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWFMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGIKGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(10626..10967)
                     /gene="yebY"
                     /locus_tag="ACHK3A_002097"
     CDS             complement(10626..10967)
                     /gene="yebY"
                     /locus_tag="ACHK3A_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO8740177.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(10980..11852)
                     /gene="copD"
                     /locus_tag="ACHK3A_002098"
     CDS             complement(10980..11852)
                     /gene="copD"
                     /locus_tag="ACHK3A_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO8740178.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGIWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLMLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(11856..12230)
                     /gene="yobA"
                     /locus_tag="ACHK3A_002099"
     CDS             complement(11856..12230)
                     /gene="yobA"
                     /locus_tag="ACHK3A_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO8740179.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            12369..12599
                     /gene="holE"
                     /locus_tag="ACHK3A_002100"
     CDS             12369..12599
                     /gene="holE"
                     /locus_tag="ACHK3A_002100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO8740180.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            12701..13357
                     /gene="yobB"
                     /locus_tag="ACHK3A_002101"
     CDS             12701..13357
                     /gene="yobB"
                     /locus_tag="ACHK3A_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO8740181.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVSAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            13381..14043
                     /gene="exoX"
                     /locus_tag="ACHK3A_002102"
     CDS             13381..14043
                     /gene="exoX"
                     /locus_tag="ACHK3A_002102"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO8740182.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(14040..16100)
                     /gene="ptrB"
                     /locus_tag="ACHK3A_002103"
     CDS             complement(14040..16100)
                     /gene="ptrB"
                     /locus_tag="ACHK3A_002103"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO8740183.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(16309..16968)
                     /gene="yebE"
                     /locus_tag="ACHK3A_002104"
     CDS             complement(16309..16968)
                     /gene="yebE"
                     /locus_tag="ACHK3A_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO8740184.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(17295..17651)
                     /gene="yebF"
                     /locus_tag="ACHK3A_002105"
     CDS             complement(17295..17651)
                     /gene="yebF"
                     /locus_tag="ACHK3A_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO8740185.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQPDPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(17718..18008)
                     /gene="yebG"
                     /locus_tag="ACHK3A_002106"
     CDS             complement(17718..18008)
                     /gene="yebG"
                     /locus_tag="ACHK3A_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO8740186.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            18142..19320
                     /gene="purT"
                     /locus_tag="ACHK3A_002107"
     CDS             18142..19320
                     /gene="purT"
                     /locus_tag="ACHK3A_002107"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO8740187.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFREAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGIQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAGQVKVQG
                     "
     gene            complement(19376..20017)
                     /gene="kdgA"
                     /locus_tag="ACHK3A_002108"
                     /gene_synonym="eda"
     CDS             complement(19376..20017)
                     /gene="kdgA"
                     /locus_tag="ACHK3A_002108"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO8740188.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(20054..21865)
                     /gene="edd"
                     /locus_tag="ACHK3A_002109"
     CDS             complement(20054..21865)
                     /gene="edd"
                     /locus_tag="ACHK3A_002109"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO8740189.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(22100..23575)
                     /gene="zwf"
                     /locus_tag="ACHK3A_002110"
     CDS             complement(22100..23575)
                     /gene="zwf"
                     /locus_tag="ACHK3A_002110"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO8740190.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            23913..24782
                     /gene="hexR"
                     /locus_tag="ACHK3A_002111"
                     /gene_synonym="yebK"
     CDS             23913..24782
                     /gene="hexR"
                     /locus_tag="ACHK3A_002111"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO8740191.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAIVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            24910..26352
                     /gene="pyk"
                     /locus_tag="ACHK3A_002112"
     CDS             24910..26352
                     /gene="pyk"
                     /locus_tag="ACHK3A_002112"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO8740192.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(26484..27455)
                     /gene="lpxM"
                     /locus_tag="ACHK3A_002113"
                     /gene_synonym="msbB"
     CDS             complement(26484..27455)
                     /gene="lpxM"
                     /locus_tag="ACHK3A_002113"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO8740193.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(27575..28897)
                     /gene="mepM"
                     /locus_tag="ACHK3A_002114"
     CDS             complement(27575..28897)
                     /gene="mepM"
                     /locus_tag="ACHK3A_002114"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO8740194.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(28913..29845)
                     /gene="znuA"
                     /locus_tag="ACHK3A_002115"
     CDS             complement(28913..29845)
                     /gene="znuA"
                     /locus_tag="ACHK3A_002115"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO8740195.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            29924..30679
                     /gene="znuC"
                     /locus_tag="ACHK3A_002116"
     CDS             29924..30679
                     /gene="znuC"
                     /locus_tag="ACHK3A_002116"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO8740196.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRHELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            30676..31461
                     /gene="znuB"
                     /locus_tag="ACHK3A_002117"
     CDS             30676..31461
                     /gene="znuB"
                     /locus_tag="ACHK3A_002117"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO8740197.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAIMFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(31611..32621)
                     /gene="ruvB"
                     /locus_tag="ACHK3A_002118"
     CDS             complement(31611..32621)
                     /gene="ruvB"
                     /locus_tag="ACHK3A_002118"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO8740198.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(32630..33241)
                     /gene="ruvA"
                     /locus_tag="ACHK3A_002119"
     CDS             complement(32630..33241)
                     /gene="ruvA"
                     /locus_tag="ACHK3A_002119"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO8740199.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         7972 a         8607 c         8320 g         8458 t
ORIGIN      
        1 caggcacaat ttcttgtgcc tgattgatat tacttgacgg gagcgggttg ttccgcgggt
       61 ctggcttttt caagcttcgc cagatcgagt gcgatattca ccgtctcatc cagataagga
      121 tccggctcct ggtaatcttt cggtagatca tccagtttct tcaactccgg tttaccttcg
      181 cgtttaaagc gttcgttcaa acgcgccaga cgcgtcgcat catcttcatt attctctttc
      241 tcacgcacag cgtaattcag agaaacgata ttgcgcttgt ccttcatggc gttgaagcgc
      301 gcgatatcct tcatgatgtt ctggaactca ggatctttcg cgatacgcgc attatgttcc
      361 ttcagcagct ccggtccaaa ggccgttaaa tcgcctgatt tcacataggt cgcggcatcg
      421 atgctatccc acggcagcgc gttatcttcg aatttctcac ccgtttccgt ttcttcatta
      481 cccgtcggca tgatgatgtc tggcgttaca cctttacgtt gcgtactgcc gccgttaacg
      541 cgatagaatt tctggatcgt gtactgtaca gatcccagcg ctggccattc aggacgtaac
      601 atctgatcgt agatacggtt caatgaacgg tattgctgaa cggtgccttt accaaacgtc
      661 ggttcaccca caaccagcgc acgaccgtaa tcctgcattg ccgcggcaaa gatttctgaa
      721 gccgaagcac tgaaacggtc aaccagtacc accagcgggc ctttatagaa gacctgtccg
      781 tcggtatcgc tgtcttcacg aactttaccg ttgttatcac ggacctgaac aatcgggccg
      841 gatggaataa acagaccgga gagcgatacc gcttcggtca gcgccccacc accattgcta
      901 cgcaggtcga tgatgacgct gctgacattc tgtttttcca gtttctgcag ttgcactttg
      961 acatcgtctg tcaaacccac atagaagccc ggaatatcca gcacgccgac tttctcttta
     1021 ccgacggtct tcaccgacat tttaaccgcg cggtcttcga ggcgaatacg ttcacgggtc
     1081 aacgttacag tacgggtctt ggtcccttta ccagcaggta aaatttccag acgaacttta
     1141 ctgcccttcg gccctttaat taaggcaacc acatcatcaa gacgccagcc aatcacgtca
     1201 accatcggct tgcctgtttg accaacaccg acaattttgt caccaacgct gatagcttta
     1261 ctcttcgctg ccgggcctcc tgccaccatc gaattgataa cggtgtagtc atcatccatt
     1321 tgcagcactg cgccaatgcc ttccagcgac aaactcattt cagtgttgaa ctgttcggta
     1381 ttacgcgggg aaagatagtt ggtatgcggg tcgatttcac gcgcaaacgc cgtcattgcc
     1441 agcgagaaaa catcttcgct gttggtttgc gccagacgac gaatggcaaa tttgtagcgg
     1501 cgagtcaggg tttcacgaat ttctttatcc gtttttcctg ccagcttcag gcttaactcg
     1561 tcgaatttga ctttactgtc ccacagcgcg ttcaactcag cctcgttttt cggccagggc
     1621 gctttgctgc ggtcaaggtt ataagtgtcg ttgccggtga aatccatcgg cttttccagt
     1681 accgacaaag cgtactggta acgttcaaaa cgacgctttt gcgccagatt gtagagatcg
     1741 tagaaaacgt cgagtttgcc tgaacgcagt tcatcgccta actcggtttt ctttttcgcg
     1801 aactgttcaa catcgcttgc caacagcacg ttgtggctgt aatcgagcag attcaggtag
     1861 cggtcaaaga ttttggccga aaatgtctga tcgaggtcga actggcgata atgagaacgg
     1921 gtgaagcgcg acgttacgcg ctcacttacc gtcgcatgct gcgtctcttc ctttaatacc
     1981 ggaatttgat cagcacgcgt gatatcttct acagcgaagg tctggcctgc tattgcaagc
     2041 aggccagcta acgcggtaag cctaaaaaac atgttcatgc ctggcccggc ctccgtttca
     2101 gaacaccagg tgttctgcgc gcacaatcaa agacataccc gaattcagct ggacgcggac
     2161 gccgtctttg gtgatttcta atacggtggc atccatcgcg ttttgacccg ctttcacctt
     2221 cagggcttgt ccgacagtca gagctgaaat gtcagaaacc ggggtgtgct gttcttcgcg
     2281 aggtgctttt actgtttttg gcgctttctc taccggcttt tgcgcacgag gtttacgttc
     2341 agcgccttct ttgcggcgtg gcgtggtcgg acgtggcttg cgttcgcggc gcggtgcgtc
     2401 ttctttctca ccagcagctg cggcagcttc gcgttttttc gcttgctgtt cagcacgctg
     2461 tgcctgaaca cgcgctttcg cttcttcaag ctgcttgcga gcatgctcta catgttgctc
     2521 gtccagctca ccgcatgggt tgccgtcaag atcgacacgc gttgcgccgg gtttaacacc
     2581 gtaaagataa cgccagctcg aagtgtagag acgtaaagcg gatcgcaatt gcgttttgct
     2641 caggttcatt tccccggcaa cacgatcgac caaatcctga aaaataccga ttttcagcgg
     2701 acgcgcttca ccttccgcac tgaaacagtg gggaaaacgt tcggccagaa acgcgattac
     2761 ttctttactg ctattcaact taggttgatt ttccatgaaa tttcctgatt acaacggacg
     2821 tagccaacaa gcgcaggcat gaacaggcgt cattataatg acgctatcag taaatgctac
     2881 gttatccgtt gattatcctg cgacgctcgc aaagaatttt ttgtaatccg tcgttgcaag
     2941 cactttttca agctgtgcca caagctgacg taagccttgc tcgtcctcgt ctgtaaagcg
     3001 accgaagacg gtactatcga tgtcgagaac accaataatc tgatttttca ccaccagcgg
     3061 cagaacaatt tcagaattac tcgccgcatc acaggcaata tgcccgtcaa acacatgaac
     3121 atcctcgata cgctgcactt gattgcgggc aaccgcagtg ccgcacacgc cgcgcccgac
     3181 gggtatccgg acacaggcaa ttttgccctg aaatggtccg agtaccagtg tatcgtcctc
     3241 aagcaaataa aaacctgccc agtttacgtc agtgagacgc tcatataaca acgcactggt
     3301 gttcgcaagc gttgccagaa aactggtttc tcctgccatc agcgcgttaa agtcgcgatt
     3361 taaatccgcg taaaattcta ttttgttcat tatgtaatca cttggttgtc ttaccaggaa
     3421 ctgccagcct attaaaataa gcattaaatg cgttaatgct caagatcatt ccaatcatgg
     3481 gttaagatta atgttaattc tttttacatt tggcacgtca tggctcttaa cacaccacaa
     3541 attacgccga caaaaaagat aacagtgagg gctatcggtg aggaactgcc gcgtggtgat
     3601 taccaacgtt gcccgcaatg tgacatgctg tttagcctgc ccgagataaa ttctcatcaa
     3661 agtgcctatt gtccgcgctg tcaggcaaaa attcgtgacg ggcgcgactg gtcgctaacg
     3721 cgcctggcgg caatggcctt caccatgctg ttgctgatgc cgtttgcctg gggcgaaccg
     3781 ctgttgcata tctggctgtt aggcattcgt attgacgcca acgtcatgca aggcatctgg
     3841 caaatgacca aacagggcga tgcgataacg ggatcaatgg tctttttttg cgttattggt
     3901 gcccccctca ttctggtgac ctccatagct tatttatggt ttggtaaccg actgggaatg
     3961 aatctacgcc cggtactgct gatgcttgag cgacttaaag agtgggtaat gctggatatc
     4021 tacctggtcg gcattggcgt tgcttctata aaggtacaag attatgccca tatccaggcg
     4081 ggtgtcggct tgttctcttt tgtggcgttg gtgattttaa cgacggtgac gttgtcacat
     4141 ctcaatgtcg aagaactgtg ggagcgattt tatccacagc gccccactac gcgtagagac
     4201 gagaaactcc gtgtctgtct tgggtgccat tttaccggct atcctgatca gcgtggtcgc
     4261 tgcccgcgtt gccatatccc gctacgcctg cgtcgccgtc atagtctgca aaaatgctgg
     4321 gcggcgctgt tagcgtcaat cgttttgttg ttacctgcca acctgttgcc tatttctatc
     4381 atttatctga atggtggccg gcaggaagat acgattcttt ccgggattat gtcgctggca
     4441 agtagcaaca ttgccgttgc gggaatcgtg tttatcgcca gtattctggt accgtttact
     4501 aaagtgatcg tcatgttcac tttactgttg agtattcatt ttaaatgcca gcaaggttta
     4561 cgcacacgca ttctgttact gcggatggtg acctggattg gtcgctggtc gatgctcgac
     4621 ctgtttgtca tatctttaac catgtcgctg attaatcgcg atcagatcct cgcttttact
     4681 atgggaccgg ctgcgtttta tttcggcgca gcggtaattt tgactattct tgctgtggaa
     4741 tggctggaca gccgcttact ttgggatgca catgagtcag gaaacgcccg cttcgacgac
     4801 tgaagcgcag attaaaaata aacgccgtat ctcacctttc tggctgctgc cgttcatcgc
     4861 gctaatgatt gccggttggc tgatttggga tagttatcag gaccggggta ataccgtcac
     4921 catcgacttt atgtcggcgg atggtattgt cccgggccgc acccctgttc gttatcaggg
     4981 cgttgaagtc ggaactgtgc aggatatcag cctgagcgac gatcttcgta agattgaagt
     5041 caaggtcagc atcaagtctg atatgaaaga tgcgctgcgc gaagagacac agttctggct
     5101 ggtgacgcca aaggcctcac tggcaggtgt ctccgggctg gatgccctcg tcggtggtaa
     5161 ctatatcggc atgatgccag gtaaaggtaa agagcaggat cactttgtcg cactcgatac
     5221 ccaaccgaag tatcggctgg ataatggcga tctgatgatc cacctgcaag cccccgatct
     5281 cggttcactg aacagcggtt cattggtcta tttccgtaag atcccggtgg gtaaagtcta
     5341 cgactatgcc atcaatccca ataagcaagg cgtggtgatt gatgtcctga tcgagcggcg
     5401 ttttaccgac ctggtgaaaa aaggtagccg tttctggaac gtttccggcg ttgatgccaa
     5461 cgtcagtatc agtggcgcga aggtgaaact ggaaagtctg gcggcactgg ttaacggtgc
     5521 gattgccttc gattcacctg aagagtcgaa acctgccgag gcggaagata cctttggtct
     5581 gtatgaagat ctcgcccaca gccagcgtgg cgtaataata aaactggaac tgccgagtgg
     5641 ggccggatta accgccgact cgacgccgtt aatgtatcag gggctggaag tcggacagct
     5701 gactaaactg gatttaaatc ctggtggtaa agtcaccgga gaaatgaccg ttgatcccag
     5761 cgtcgttacg ctgttacggg aaaatacccg catcgaatta cgtaatccga aattatccct
     5821 tagcgatgcc aatctcagcg ccctgctgac cggaaaaacc tttgagttgg tgcccggcga
     5881 tggtgagcca cgcaaagagt tcgttgttgt gccaggcgaa aaagccctgc tacaagagcc
     5941 ggatgttctg acactgaccc tgacagcacc agaaagttac ggtattgatg ctggtcagcc
     6001 gctcattctc cacggtgtgc aagtaggcca ggttattgat cgtaaactca ccagcaaagg
     6061 cgttaccttt accgtcgcca tcgagcctca gcatcgcgaa ctggtaaaag gcgatagcaa
     6121 atttgtcgtc aacagccgtg tcgacgtgaa ggtggggctg gatggcgttg agtttctcgg
     6181 tgccagcgcc tcagaatgga ttaacggcgg gatacgtatt ctgccgggcg ataaaggcga
     6241 gatgaaagcc agctatccac tgtatgccaa tctggaaaaa gcgctggaga acagccttag
     6301 cgatttaccc accacaaccg taagtttgag tgcagagacg ctgccggatg tgcaggcagg
     6361 atcggtagtg ctgtaccgta aatttgaagt tggtgaagtt attactgtgc gtccgcgagc
     6421 taacgcgttt gatatcgatc tgcatattaa gccggagtat cgcaaccttc tgaccagcaa
     6481 cagcgtgttc tgggcagaag gtggggcgaa agttcagcta aatggtagtg gtctgaccgt
     6541 acaggcatcc ccgctctcca gagcattaaa gggggctatt agcttcgata acctcagcgg
     6601 tgccagcgcc agtcagcgta aaggcgacaa gcgtattctg tatgcttccg aaacagcggc
     6661 ccgtgcggtt ggcgggcaaa ttacgcttca cgctttcgat gccggaaaac tggcggtcgg
     6721 gatgccaatt cgctatctcg gtattgatat cgggcaaatc cagacgctgg atctgattac
     6781 cgcgcgcaat gaagtacagg caaaggcggt actttacccg gaatatgtcc agacctttgc
     6841 acgcggtggt acacgcttct cagtggtcac accgcaaatt tcggcagctg gcgttgagca
     6901 tcttgatact atcctccagc cgtatatcaa cgtcgaacca ggccggggca atcctcggcg
     6961 cgactttgaa ttacaagagg ctaccataac tgattcgcgt tacctggatg gcttaagcat
     7021 tattgttgaa gcgccggaag ccggttcgtt aggcatcggt acgcctgtgc tgttccgtgg
     7081 tctggaagtc ggtacggtta ccggaatgac gctggggaca ttgtcagatc gcgtgatgat
     7141 tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt aacaattccg tcttctggtt
     7201 ggcatcgggt tacagtctgg actttggtct gacgggcggc gtagtgaaaa ccggcacctt
     7261 taaccagttt atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc cactggcacc
     7321 gaaagcccag gaaggcaagc acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga
     7381 atggggaact gcgcttccca aataatgccc actgctccgg cgtgcctgcg ccggagcgtt
     7441 tatgctaaac tgcgcgcctg tttttttgcc agtggtatat gctcgtggcc caacacaccg
     7501 tttatttccc ggacgccttt ctgacacaaa tgcgcgaagc gatgccttcg acgctctctt
     7561 ttgatgattt tcttgccgcc tgtcagcgcc cgttgcgccg cagcattcgc gttaatacgc
     7621 tgaaaacctc cgttgctgat ttcctgcaat taactgctcc ttatggctgg acgcttacgc
     7681 caattccgtg gtgtgaagaa ggtttctgga ttgaacgcga cagtgaagat gcattgccgt
     7741 tgggtagtac cgccgagcat ttaagcggcc tgttttatat tcaggaagcc agttcaatgt
     7801 tgcctgttgc cgccttgttt gctgacggta atgcaccaca gcgggtgatg gatgtcgctg
     7861 ccgcgcccgg ctccaaaacg acgcaaattg ccgcgcggat gaataacgaa ggggcaatcc
     7921 ttgccaatga gttttccgcc agtcgggtaa aagtgttaca tgccaatatc agccgctgtg
     7981 gcatcagtaa tgttgcgctc acgcattttg atggccgcgt atttggtgcg gcagtgccag
     8041 aaatgttcga tgccattttg ctggacgctc cctgctccgg cgaaggcgtg gtgcgtaaag
     8101 atcccgatgc gctaaaaaac tggtcaccag aaagcaatca ggaaatcgca gcgacccaac
     8161 gggaactgat cgacagcgcc tttcatgcat tacgtcctgg cggtacgctg gtttactcga
     8221 cctgtacctt aaaccgggaa gaaaacgaag ccgtttgcct gtggctgaaa gagacttatc
     8281 ccgacgcagt agagttttta ccgcttggcg atctcttccc tggtgcaaac aaggcgctga
     8341 ccgaggaagg ctttttgcat gttttcccac aaatttacga ctgcgaaggc ttcttcgttg
     8401 ctcgtctgcg taaaactcag gcgattcccg ccttacccac cccaaaatac aaagtgggca
     8461 atttcccgtt cagcccggtg aaagatcgcg aagccggaca aattcgtcag gcggctgcaa
     8521 gtgttggctt aaactgggat gaaaacctgc gactctggca gcgtgacaaa gaactgtggt
     8581 tgttcccggt gggcattgaa gccctgatcg gtaaagtccg attttctcgc ttggggatta
     8641 aacttgccga aacgcacaac aaaggttatc gctggcagca tgaagccgtt attgcccttg
     8701 cctcccccga caatgtgaac gcttttgaac tgacaccgca ggaagcggaa gagtggtatc
     8761 gcgggcgcga tgtttacccg caagccgcgc cagtagcgga tgatgtattg gttactttcc
     8821 agcatcagcc gattggttta gccaaacgga ttggttcgcg actgaaaaac agctacccgc
     8881 gtgaactggt gcgcgatggg aaacttttta ccggtaacgc ctgacagcgc acaaaaaaag
     8941 cgcacttttt gactggcaca ttcggctgcc tcaactaggc tgaaaaatgg tgcgatcgga
     9001 ctggtcgtac cacaaccggc agctaaatgg agagcacgaa gatgaaaacc agtgtgcgca
     9061 taggcgcttt tgaaatcgac gacggcgaat tacacggcga atcgccgggt gatcgaacgt
     9121 taaccattcc ttgtaaatca gaccccgatt tatgtatgca actggatgcc tgggatgctg
     9181 aaaccagtat tcctgccctg ctaaatggcg aacactctgt cctttaccgt acccgttacg
     9241 atcaacaatc tgatgcctgg attatgcgtc ttgcctgatc caaaaagaac ccgtcggcat
     9301 ggcgggttat ttgtcctggt tatttccccg ttgtaaaatc tctcctaaac ttaacggtac
     9361 ggcaccacac ttcggggatg aaatgttcgc gctggtactt tttgtttgct acctggatgg
     9421 cggttgtgaa gatattgttg tggatgtcta caacacggaa cagcagtgtc tttattctat
     9481 gagcgatcaa cggatccgcc atggcggttg ttttccaatc gaggatttta ttgatggttt
     9541 ctggcgacca gcacaggagt acggtgattt ttaattattg caattgcaca agagtcagtt
     9601 cgcccccaaa gacagcaccg gtatcaatat aatgcaggtt gccaatatcc acgcgatgtc
     9661 gcaacggtgt atgaccaaac cagaaatgat cagcaccttt aattccctgc ccttttgggc
     9721 gttcacctaa tcgcgagcgg ctccacaaga cctgatgcaa atcaacgtcc ttttgccatt
     9781 cataaacatc atccggataa tcggcatggg caataacatg tttgccggtg cgactgtgta
     9841 cttcaagaat aaagggcaaa tgctgacatt tttccagcgc cgttttcgct tgtttctgtt
     9901 gattatctgc cagcgcaata aaccagtcgc cgccattcat gaaccacaaa gacatctgct
     9961 gggatgccag cgcatccatc gccatctgtt catgattgcc tcttaccgca cgaacccaat
    10021 gttgttccag taactgcagg caacgtaaac tttgcggccc acgatcgata acgtctccca
    10081 ctgagataag taaatctcgc cacggatcaa aacgacaatg ccataatttg cggcgcaact
    10141 gctcaagaca accgtgtata tcgcctgaaa gccagatatg tcgccattga tgacccgcaa
    10201 ttctctgata aacgggcgca ggctgtttca tcaatatttt cctcccgcgc taaagatcac
    10261 ataatcttaa caagaatgtt aaaaaacgct ggacgcagac agtagagtgt gtgttatggt
    10321 tgactataaa gtcagcgaag ggaatgcttc tggcttttaa cagataaaaa gagaccgaac
    10381 acgattcctg tattcggtcc agggaaatgg ctcttgggag agagccgtgc gctaaaagtt
    10441 ggcattaatg caggcttagt tgccttgctc tttaagaata gatgacgacg ccaggttttc
    10501 cagtttgcgt gcaaaatggt caataaaaag cgtggtggtc atcagcttaa atgttaaaaa
    10561 ccgcccgttc tggtgaaaga actgaggcgg ttttttattg gaaatcaaaa ggctatttta
    10621 ggtaattaac agagtttttc agctcgctct ataaacggtg ccagactcat tttttcgccg
    10681 ggattgttag gatcatcaat ctgaatcacc gaaatgggtt gggctttagt cttcccactg
    10741 gcaacttcct tttgtgcgat atcgtttaaa ggatactgca cgagggtact tgggttgatg
    10801 acatacaaag cattacccgg tcggcaagtc agcatcacct cttcgcgatt aaacgcccat
    10861 ttgtctttac ccacttcaaa acggctgacg gtaatcacct gcggtgccgc cagcgccgct
    10921 gcagaactgg tgagtaacag aaacgccaga atactttttt tcatcatttt cgtaatccat
    10981 caaaaaggtt cgagagtcgc aagcaggctg atgatcacca gcactaccgc acctatcgcc
    11041 cattcgagtt tagtcatcca cacaaaccag ggagccgcac gatcttcatc ctgcctcatg
    11101 cgcggtacaa gaacataacg attagccagc gcgatgacca ccataatcat caccaggatg
    11161 gctttgagca ttaagagttg gccccagtaa gtcgtaagcg tggggggaaa tccggtaatc
    11221 aacaatgcat taagcacgcc gcttgccagt acgccgatca ccgcaaaatg cccgcaccag
    11281 gaaaaacgca tcagcgcctg aatagcctgg tgtcgccaac ggcctttgat gagttgcata
    11341 caccagagca ccggcagtaa tcccccaaac cacgccgccg cacagatcag gtgaattgcg
    11401 tgattagtct gatggatttt cgccgttacc ccttcattca gcgtcgcatg cccgactccc
    11461 gccagcagaa taaattgcgc ggtagtgagc ataaatagca aacgtggcat attctgcggt
    11521 tgcataaggg caacgatcaa cgttacgagg gcgagaacaa tttgccataa ccagatgcca
    11581 ccaaactgcg tttgcaaaac agccagccaa atgtttggcg aaaatacatc tgaccatccc
    11641 gttcccatca acccgccctg aacggcgagc attgcagtgg cactgatcaa actccagacg
    11701 gcagcgtgct gttgtaaacg cagaaaacgc ttcgtcagca agcgacgaat cgttaagggg
    11761 gccagccagg caccgtacat tgcgaagcca aaaaccagca tcaacgaggt gaaatggata
    11821 aatcgcagcg cgatccaggt aaacgccagc atgatttatt tcacgctaaa ggtgtagtgt
    11881 cctttcgttt tatgcccgtc caccgaaaca acatgccagt caacggtgta ggttccgggt
    11941 ttcagcgaat cggccagtgg aacaatcagt tgcttttggt cctgctcatt ccgcttcgcc
    12001 ggtaatgttt taatattttc gttttttggc cccgtgattt ttgcaccact gaatcctgtt
    12061 tcaacgcctt ccgagaagtt taaagtgatt gcctgcgggg cagctgtcac ttgcgcattt
    12121 gccgcaggat actgatgcgt taaatgcgca tgtgcccaga cggaaggtgt caccaacgaa
    12181 gtggtcagga ttgccagcgc gtagcgaagg gagcgtgcag ttgaagccat attatctatt
    12241 cctttttgta ataacttttt tacagagcat aaccttgtct aatgtctgag tcgaggatca
    12301 tcaattccgg cttgccatcc aggctcactc ttagtaactt ttgcccgcga atgatgagga
    12361 gattaagaat gctgaagaat ctggctaaac tggatcaaac agaaatggat aaagtgaatg
    12421 tcgatttggc ggcggccggg gtggcattta aagaacgcta caatatgccg gtgatcgctg
    12481 aagcggttga acgtgaacag cctgaacatt tgcgcagctg gtttcgcgag cggcttatcg
    12541 cccaccgttt ggcttcggtc aatctgtcac gtttacctta cgagcccaaa cttaaataaa
    12601 acttatacag agttacactt tcttacataa cgcctgctaa attatgagta ttttctaaac
    12661 cgcacccata atttgcagtc attttgaaaa ggaagtcatt atgtcgttct ggaaagttgc
    12721 agcagcgcaa tatgagcctc gtaaaacctc actgaccgaa caggtagctc atcatctgga
    12781 gtttgtgagt gccgccgcca ggcaacaatg tcagctatta gtttttccat cgctttcttt
    12841 actggggtgt gattattcgc gacgtgccct tcctgcccca cccgatctct cgctgttaga
    12901 cccgctttgt tatgctgcaa cgacctggcg aatgaccatc attgccggcc ttcccgttga
    12961 atataacgat cgctttattc gtggaattgc ggtgttcgca ccgtggcgaa aaacaccagg
    13021 gatctaccat cagagtcatg gtgcatgtct gggccgtcgt tccagaacca tcacggtggt
    13081 cgatgaacaa ccggaaggta tggatatgga cccgacctgt tcactgttta cgacagggca
    13141 atgtctgggg gaacccgacc tactggcatc tacccgccgc ttacaatttt tttcacatca
    13201 gtactcaatt gccgtgctga tggcaaatgc ccgtggtaac agtgcactat gggatgaaca
    13261 tggtcgtctc atcgttcgcg ccgatcgtgg ttcattattg ttagtcggtc agcgttcatc
    13321 acagggttgg caaggcgata tcattccatt acgctaggct ttttcggcct ggagcatgcc
    13381 atgttgcgca ttatcgatac agaaacctgc ggtttgcagg gagggatcgt tgagattgcc
    13441 tctgttgatg tcattgacgg aaaaatcgtc aaccccatga gccacctggt gcgccccgat
    13501 cgtcctatta gtccacaagc gatggcgatt catcgcatca ccgaagccat ggtcgccgat
    13561 aaaccgtgga ttgaagatgt gatcccacac tattacggta gtgaatggta tgtcgcgcac
    13621 aacgccagct ttgaccgccg cgtactgcct gagatgcccg gtgagtggat ttgcactatg
    13681 aaactggccc gtcgtttgtg gccagggatc aagtacagca atatggcgtt atataaaaca
    13741 cggaagctca atgtacagac gccgccgggc ctgcatcatc accgcgcgtt gtatgactgt
    13801 tatatcaccg ctgcgttgct tatcgatatt atgaacacct ccggctggac ggcagaacag
    13861 atggccgata tcaccggacg tccgtcgttg atgacgacct tcacctttgg caaataccgt
    13921 ggcaaagcgg tttccgacgt tgccgaacgc gatccgggct atctgcgctg gttgtttaat
    13981 aacctggaca gcatgagccc ggagctgcgt ttaacactga aacattatct ggaaaatact
    14041 taatcctcag gcgtagcggg taatgttccc tgcgccagcg cgaccagaaa agcatattcc
    14101 atcgctacgc cttcgtacga tttaaagcga ccagatttac cgccatgacc tgagtccatg
    14161 tcggtacaga gcaataaaag atggtcatcg gttttcagct cgcgcaattt agcgacccat
    14221 tttgccggtt cccaatattg cacctgagaa tcgtgcaaac cggtcgttac cagtaaatgc
    14281 ggataagcct gtgcggtgac gttgtcatat gggctgtagc ttttcatgta ctcgtaatat
    14341 tgcggatcct gcggattccc ccactcttca aactcaccag tggtaagagg aattgattca
    14401 tcaagcattg ttgttacaac atcaacaaac ggtacctggg cgataacgcc gtggaataat
    14461 tccgggcgtt gattaattgc aacgcccatc aacatgcccc ccgcacttcc gcccatcgca
    14521 taacagagcg aaggagagcc atagcccagt tttaacaatg catcgcaggc atcaagataa
    14581 tcattaaacg tatttttctt cttcagaaat ttaccgtctt cgtaccattg ttgccccagc
    14641 tcaccaccgc cgcgaacatg gacaatggcg tagacaaagc cacgatctaa caaactcaag
    14701 cggctaaaac tgaaatcggc atcaatactt gcgccgtaag aaccgtagcc atacaccagc
    14761 agcgggttgt gtcctttgcg aaaatgtttg cgatgataga ccagcgaaac cggaacttcg
    14821 acgccatcac gggcgactat ccacaggtgt tcactgcggt aattcgccgc atcaaaacca
    14881 ggaacttccg tttgttttaa tacacgacgc tcaccggtat ccatatccag ttcaaacaaa
    14941 gtatctggtg tggtcatgga agaataacca taacgcaatc gcgcggtttc aggttctgga
    15001 ttgtaggcaa tccaggtcac ataggccgga tcatcaaagg caatgccaat gacttcccgg
    15061 gtcttgcggt tgatttggcg caaactggtt aacccgcgct gacgctcttc aaccaccagc
    15121 cagtcggtaa acagcgtaaa cccttccagc ataatgttat cacgtggcgg aattaactct
    15181 tcccactgtt gctcatcacg catacgggta cggtataagc caaagttttt accgtggcgg
    15241 ttggaacgca gataaaaccg atgctggtag tgatcaaggc tgtattcgtg atctttgcgg
    15301 cgaggcagaa aaacaaacgg ctcggcatcg gccatttccg cgtccagcag gcgaacttca
    15361 ctggtggtgg cgctggccag atgaatgacc acataatgct tcgaggtcgt tttatgcagg
    15421 ctgacgtaat aggtatcgtc tttttcttcg taaatcagtt tatcttgcga tgctggcgta
    15481 ccgatggcgt gacgccagac ctgataaggc agcagcgtca ctggatgctt gcgaacatag
    15541 tagaaagtcc aggagtcatt tgcccagaca aagctgggtt caacgttatc cagcagttcc
    15601 gggtaccagt tacctgtttc cagattacga aaacgaatgc catactgccg tcgggaaaga
    15661 aaatcttctg ccagtgccat aatggtgtta tctggcgtaa tcgccattcc gcccatcgaa
    15721 taaaactcac tatgagccgc gcgcttgttg gcatcgagca atgtttccca ctcgtcccac
    15781 tcttcactga acgccgattg acgctggtag atagcatatt cacagcctgg ttcataaata
    15841 tgccgatagc ggtagccatt tttgatgtag ggcgcagaaa cttctcgttg cggaatgcgg
    15901 tcgatgattt cctttaagat gcgatcctgc aaggcttgtt gcgaggccat cacccgatga
    15961 ccgtaactat tttcttgttg cagatagtcc aggactttcg gctgagaacg cgtatcgtcc
    16021 cgcagccagt agtaattatc gatgcgcgta tcgccatgaa gcgtcatggc gtggggaatg
    16081 cgggcggctt ttggtagcat gttattgttc tttctggttg aaacatctta taagggtggc
    16141 aaaactcacc ggggatgcaa gcgaaacagg gtagtcattg cttagatgat gacaggtaat
    16201 ggcgcggata tcgaatgtta tgcagacaga gaaaatcagc ctgctcaaat ggctgtgcgg
    16261 ttctggataa cccgaaataa tcagcttcag gctatccaga gagcggaatt actccgccaa
    16321 agtacgtttt tgctgttcga gatcgcgttc aatgccttca cgaacatcct gggggatttt
    16381 cagcgcatca cccagtgcat tcaggtaact gcgttccata aaatggtcaa tatcaatagc
    16441 cgcgcagctc aggaaataga tttccagcgc ctcttcttca ttgcggaccc cggtagccag
    16501 gcgttgtgga tccagcggtt gttcgattgc ctgctcaatg agtacacgcc ccttctcttc
    16561 cacaccggct tctcgcaatt gctggtcgat tgccgcacgt tctttggcat caatatggcc
    16621 atcactttta gcggcaaaga ccagcgcaag gatcaaacgt tccgtacgct catccagcgg
    16681 cgtactttgc gcgccaaact gcggttcatc ctgatgcgcc gcgcgaattt tatctttgta
    16741 tttattccac agcaccgtac ccgctaccgc tccgccgcca accagtaacg cgtttgtgcc
    16801 atatttcgta agaagtttac gtgctgattt atttgcgacc agcagcccag ccagtccgcc
    16861 taatgcgcca ggcactaaaa gtttgcccaa tccctgatcc gcagacgagg aggtagaaga
    16921 acggctttgc ccaagcaggg attgcagttg atttaaccag ttagccatat ttgctcctca
    16981 ataaccattt ttagtgcctg tagcatagcg gatgaggatg tcagaaaatg tatattgaga
    17041 caaaagatgc gcaaataagc ggggcggtag gccgttattc aaagaaattc gcgccaggta
    17101 aggtgttcac tgtatataag gtgataaggg tattttaggt gaaagtagtt tgacgttttg
    17161 agtggaactg tttgatgctg attgcccggt tcgacgctgc tgcgtcttct caggactacg
    17221 attgtgttgc gttgtagggc ggacaaggca ctcgcgccgt atccgccaac ggtcaatcga
    17281 attatcagaa gccattaacg ccgctgatat tccgccattc ccgctttgca gtccacgctg
    17341 acctggtaat gaatatcggc acttttacca cgtacggtta gcggtactga ccatttatca
    17401 tctttcccct gaatgtcctg caaactgacc catgccacgg gatcgggctg accgacaatt
    17461 ttttgatcat cagcccagcg cgccacgcga ttttgttggt aatcacgttt tacgctcgcg
    17521 gcaattccgg cagcatccag accttcacac tttgggaaag tgaccgactt gctggtttca
    17581 ttattggcag cgaaaactga tgcgcaggca gaaaccaaca acagccctaa aaacgcccct
    17641 ctttttttca tgtttttctc catagcacaa tgattcagga gaaagcatgg tacaaattgt
    17701 caggagcgca agttgcttca ggccgcgtgt gaggcatctt cctcttcgga ttcagcaccg
    17761 acgacctgtg gggacggtaa tttgccggtt ttcagaatgg tgctcagcac atctttttgt
    17821 tctgccagcc atagcgaaag ggcttcacgt tgctcgtctt ccatttgcac tggagaattt
    17881 gtcagccagg tgtcaagcag atccgccgta tcaagcattt tgtcataagc atcggcttcc
    17941 tttttgctgg taaacgacat tttctcttcg ccctcacgaa tgactacgta tttaacttca
    18001 accgccattt gcagcctctc ataataactg tgattttata cagtatattt cttctcgatt
    18061 gggaaatcaa catcaacaat aaagacacgc gcaaacgttt tcgtttatac tgcgcgcgga
    18121 ataaatcagg ggatattcgt tatgacgtta ttaggcactg cgctgcgtcc ggcagcaact
    18181 cgcgtgatgt tattaggctc cggtgaactg ggtaaagaag tggcaatcga gtgtcagcgt
    18241 ctcggcgtag aggtgattgc cgtcgatcgc tatgccgacg caccagccat gcatgtcgcg
    18301 catcgctccc atgtcattaa tatgcttgat ggtgatgcat tacgccgtgt ggttgaactg
    18361 gaaaaaccac attatatcgt gccggagatc gaagctattg ccaccgatat gctgatccaa
    18421 cttgaagagg aaggactgaa tgttgtcccc tgcgctcgcg caacgaaatt aacgatgaat
    18481 cgcgagggta tccgtcgcct ggcagcagaa gagctgcagc tgcccacttc cacttatcgt
    18541 tttgccgata acgaaaacct tttccgcgag gcggttgctg aaatcggcta tccctgcatt
    18601 gttaaaccgg tgatgagttc ctccggcaag gggcagacgt ttattcgttc tgcagagcaa
    18661 cttgctcatg catgggagta cgctcagcaa ggcggtcgcg ccggagcggg ccgcgtaatt
    18721 gttgaaggcg tcgttaagtt tgacttcgaa attaccctgc taaccgtcag cgcggtggat
    18781 ggcgtccatt tctgtgcacc ggtaggtcat cgccaggaag atggcgacta tcgtgaatcc
    18841 tggcaaccac agcaaatgag ccagctggct ttggaacgtg cgcaagagat tgcccgtaaa
    18901 gtggtgctgg cactgggcgg ttatgggctg tttggtgtcg agctgtttgt ctgtggtgat
    18961 gaggtgattt tcagtgaggt ctcccctcgc ccacatgata ccgggatggt gacgttaatt
    19021 tctcaggatc tctcggagtt tgccctgcat gtgcgagcct ttctcggact tccggttggc
    19081 gggatccgtc agtatggtcc tgcagcttct gccgttatcc tgccacaact gaccagtcag
    19141 aatgtcacgt ttgataatgt gcagaatgcc gtaggcgcag gcatacagat tcgtttattt
    19201 ggtaagccgg aaattgatgg cagccgtcgt ctgggtgttg cgctggcgac cgctgagagt
    19261 gttgatgaag cgattgagcg cgcaaagcac gccgccggac aggtaaaagt acagggttaa
    19321 acccggcaaa aaaacgctac aaaaatgccc gatcctcgat cgggcatttt gacttttaca
    19381 gcttagcgcc ttctacagct tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt
    19441 ccagcgcatc tgccggaacc agccaggaac caccgatgca cagcacgctt ttcagcgcca
    19501 ggtagtcacg gtagttagcc ggagaaatac cacctgtcgg gcagaaacgg acctgggaga
    19561 acggacccgc gatcgcctgc agggctttca caccgccgtt agcttcagcc gggaagaatt
    19621 tgaactcttt caaaccgtag tccataccca gcatcagttc ggaaacagtg ctgatccccg
    19681 gaatcagagg aatagtccct tcggtagcag ctttcagcag cggctcggtc agacccgggc
    19741 taattgcaaa ctgtgcaccc gcttcagtga cttctgccag ctgctgtgga ttcagcaccg
    19801 taccggcacc cacaatcgct tcaggcactt ctttggcgat agcacggata gcgtcaactg
    19861 cgcactcggt acgcagagtc acttccagaa cgcgcacccc accagcaacc aacgcttttg
    19921 ccatcggcac cgcgtgttcc agttttttta ccacgataac cggtacaacc gggccggtgg
    19981 tcaggattga ttctgcactt gttttccagt ttttcatcag agttttctct cgcctgatta
    20041 caaatttgtc gtcttaaaaa gtgatacagg ttgcgccctg ttcggcaccg gacagttttt
    20101 cacgcaaggc gctgaataat tcacgtcctg ttcccacgcg tgacgcgctc aggtcaggaa
    20161 tgtgtggttc gcgagcagcc agttccgctt cgtctaccag cagcgtcagt tcgcctgtct
    20221 gtccattcac acgaatgatg tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg
    20281 cttctggtgt tacgtggata gctgacggca ctttacctga agcgccggag agtcgtccat
    20341 cagtaactaa cgcaattttg aaacaccggt ccaataatac accaagtggc ggcatgagtt
    20401 tatgtaattc tggcattccg ttcgcttttg gcccctgatg acggacaaca acgacacaat
    20461 cgcggtccag caaacccgct tcaaaggccg gcataacgtc atgctggctt tcaaaaacaa
    20521 ccgcaggcgc ttcaatcacc tggttctcta ccggcacggc agaggttttc ataaccgcac
    20581 ggcccaggtt accgcttaac acttttgtcc caccatgatg agagaaaggt tgttcgaagg
    20641 aagcgatcac attgctgtcg agtgattttt ccgccccttc ccgccagtcc agttcaccat
    20701 tattcagcca tggttcaagg gtataacgag acagaccaaa gcctgccacc gtattgacat
    20761 cttcatgcag caggcctgct ttgagcagtt cacgcaccag aaccggtacg ccacctgccg
    20821 cctggaagtg gttaatatcg gccggaccgt tcgggtagag acgtgccatc agcggtacaa
    20881 catcagaaag gtcagagaag tcatcccagt taatctgaat accggctgcg cgcgccatcg
    20941 ccaccaggtg catggtgtgg ttagtggaac caccggtcgc cagcagtgca acgataccgt
    21001 tcaccaccac tttctcatcg atcatcttac cgatcggcat ccattcatta ccattaccgg
    21061 tcatgcgcgt aacctggcgc gcagctgcag cggtcaaagc atcgcgcagc ggagaatccg
    21121 gatgaacaaa agaagagcct ggcaactgca tccccataaa ctccaccacc atctggttgg
    21181 tgttggcagt accgtagaaa gtacatgttc ccggcgcatg ataagacgcg gcttctgact
    21241 ccagtagtgc catgcggtcc actttacctt cggcataaag ctggcgaata cgcacttttt
    21301 ctttatttgg caaaccgctt gccatcggtc cagacggcac aaacaccgca ggcaaatgcc
    21361 caaacgacag ggctgccatc gtcagacccg ggacaatctt gtcgcacaca ccgaggaaca
    21421 gagcaccatc aaacatgtta tgggacagcc ccaccgccgc agacatcgca atcacttcgc
    21481 ggcttaacag cgacaattcc attccatcct gcccctgggt gacaccatca cacatcgccg
    21541 gaacaccgcc tgcaacctga ccaaccgcat ttgcttcatg cagagcttta cgaatgattt
    21601 ctggatagtg ttcataaggc tggtgcgcgg agagcatgtc gttataggag gtgatgatgg
    21661 cgatattgtt acgcaacatg cttttcaaag aggctttgtc ttctggctgg caggcagcga
    21721 aaccgtgtgc caggttaccg catgccaact gcgaacgatg aacggtcgaa gttttcgctt
    21781 gttctatccg ggcgaggtaa gcagagcgag tctcgcgcga acgttcaatg attcgatttg
    21841 ttacgcgtaa caattgtgga ttcataaagg ctcctgaaat tgagttgtca gagcaggatg
    21901 attcacaaca cgtttcattc agaggattca tgactgaaac gcctgtaacc ggagctcata
    21961 gggcaaaaac gtttcagtca gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc
    22021 ctgaaagtgt aaaaattgtt ctacaatctg tgcaagatca tgttaccggt aaaataacca
    22081 taaaggatga gcgcagatat tactcaaact cattccagga acgaccatca cgggtaatca
    22141 tcgccactga ggcaacgggt ccccaggttc cggcctgata cggtttcggc gcatcattgt
    22201 ccatcgccca cgcctcagta atggagtcta cccatttcca ggcttcttcc acttcatcgc
    22261 gacgtacgaa cagtgcctga ataccacgca tggtttccag cagcagacgt tcataggcat
    22321 ctgccagatg cgtttgatta aaggtttctg aatagctcag atccagcttg gtgatttgca
    22381 ggttatgttt atggtcaagg ccaggaactt tatttagtac ctggatatcc acgccttcat
    22441 caggttgcag acggatagtc agtttattct gcggcagatc ctgccacgac tctttaaaca
    22501 gattcagttc aggtgttttg aaatagacca cgacttcaga acatttggtc ggcagacgtt
    22561 taccagtacg caggtagaat ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga
    22621 tcgccacgaa ggtttctgta ttgctgctct tgttcgcgcc ctcttcttcc agatatcccg
    22681 gcactttttt gccctgggcg aagcccgcag tatattgccc gcgtacggtt ttttcgcgca
    22741 cgttggagcg gtcaatgcgg cgcagagact tcagtacttt cactttttca tcgcggatgc
    22801 tgtctgcact caggtcagac ggcggagaca tcgcaatcat gcaaagaatt tgcagcaggt
    22861 ggttctggat catgtcgcgc atctgaccgg ctttatcaaa atagccccag cgcccttcga
    22921 tccccacttc ttctgccacg gtaatctcaa catgatcaat ggtgcgattg tcccagttat
    22981 tcacaaacag ggagttagca aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa
    23041 gatagtggtc gatacggtaa acctggcact cctcgaagta ttcgccaacc tgatcgttga
    23101 tttcctgcga ggtcgccagc gacgtcccca gcggtttctc catgactacg cgtgccggtt
    23161 tagcattcag ttttgcctca ccaagccctt tgcaaattgc gccaaaagtg ctgggcggca
    23221 tggcaaagta gttaatggtg atacgatttt tttgatccag catcgcgccg agacggctga
    23281 atgcagcagt gtcattgaca tcgagattac aaaaatccag acgtgcactc agggtgtccc
    23341 ataaaccttc atcaatggtt tctttcatga aagtttcgag cgcctcgcgg acaactttgg
    23401 tataagccgc tttatcccag tcagcacgcc ctacgccgat aatccgggtg tccgggttga
    23461 gctgaccggc tttttccagt tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc
    23521 ctttcgcgcc gaaaatgacc aggtcacagg cctgggctgt ttgcgttacc gccatgtcat
    23581 tctccttaag ttaactatcc cggtacttaa gccagggtat acttgtaatt ttcttacggt
    23641 gcactgtact gcttttacga gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta
    23701 cggtaaaagc gcgcaaagtg cggcaaaact gataaaaaaa tcatcgtttt ccattgggta
    23761 aaaatctgac actgatcatg ttatgaaaaa aaataacaac ttttttatct gcttttgtca
    23821 ttaacacagc acacaggcgt aatatctgac aaaactgcat ttcgatttct ttcagagcgg
    23881 aaatcgtcat tacccgtgag tctctttaca tcatgaatat gctggaaaaa atccagtctc
    23941 agctggaaca tttgagcaaa tctgagcgca aagttgccga ggtcattctg gcttcgcccg
    24001 ataacgcgat ccattcgagt attgctgctc tggcgctgga agccaatgtt agcgaaccga
    24061 cggtgaatcg tttctgtcgc agcatggaca ctcgcggttt tcctgatttt aaacttcatc
    24121 tggcacaaag tctggcgaat ggcactccct atgttaatcg caacgtcaat gaagatgaca
    24181 gcgttgaatc atacacgggg aaaatatttg agtccgcaat ggcaacgctc gatcatgtcc
    24241 gtcattcact ggataaatct gccatcaacc gcgccgtcga tttgctcact caggcaaaaa
    24301 aaatcgcctt tttcggatta ggctcttcag ccgccgttgc ccacgatgcg atgaataagt
    24361 tctttcgttt taatgttccg gtggtgtact ccgatgatat cgtgctgcaa cgcatgagtt
    24421 gtatgaattg tagcgacgga gacgtggtgg tgctgatttc tcacacggga agaacaaaaa
    24481 atctggtcga gctggcgcag ctggcacgcg aaaacgacgc catagtgatt gccctcacct
    24541 ctgcgggtac cccgctcgcc cgggaagcaa cgctggcaat taccctcgac gtaccggaag
    24601 atactgacat ttatatgccc atggtttctc gacttgcgca gctgaccgtg atagatgtgc
    24661 tggcgacagg atttactttg cgacgcggtg caaaattcag agataacttg aagcgggtca
    24721 aggaagcgct gaaggaatcg cgttttgata agcagctact taatttaagt gacgatcgct
    24781 aaaaacgact gtcactgtcc taatcttata cgacatccga atgagattaa tttatcgcca
    24841 tcgcggcgtt atttcattcg gatttcatgt tcaagcaaca cctggttgtt tcagtcaacg
    24901 gagtattaca tgtccagaag gcttcgcaga acaaaaatcg ttaccacgtt aggcccagca
    24961 acagatcgcg ataataacct tgaaaaagtt atcgcagcgg gtgccaacgt tgtacgtatg
    25021 aacttttctc acggctcgcc tgaagatcac aaaatgcgcg cggataaagt tcgtgaaatt
    25081 gccgcaaaac tggggcgtca tgtggctatt ctgggtgacc tccaggggcc caaaattcgt
    25141 gtatccacct ttaaagaagg caaagttttc ctcaatattg gggataaatt cctgctcgac
    25201 gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg gtatcgacta caaaggcctg
    25261 cctgctgacg tcgtgcctgg tgacatcctg ctgctggacg atggtcgcgt tcagttaaaa
    25321 gtactggaag ttcagggcat gaaagtgttc accgaagtga ccgtcggtgg tcccctctcc
    25381 aacaataaag gtatcaacaa acttggcggc ggtttgtcgg ctgaagcgct gaccgaaaaa
    25441 gacaaagcag acattaagac tgcagcgtta attggcgtag attacctggc tgtctccttc
    25501 ccacgctgcg gcgaagatct gaactatgcc cgtcggctgg cacgcgatgc aggatgtgat
    25561 gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt gcagccagga tgcaatggat
    25621 gacatcatcc tcgcctctga cgtggtaatg gttgctcgcg gcgacctcgg tgtggaaatt
    25681 ggcgatccgg aactggtcgg cattcagaaa gcgttgatcc gtcgtgcgcg tcagcttaac
    25741 cgcgcagtaa tcacggcgac ccagatgatg gagtcaatga ttactaaccc gatgccgacg
    25801 cgtgcagaag tcatggacgt agcaaacgcc gttctggatg gtactgacgc tgtgatgctt
    25861 tctgcagaaa ctgctgctgg tcagtatccg tcagaaaccg ttgcagccat ggcacgcgtt
    25921 tgcctgggcg cggaaaaaat cccgagcatc aacgtttcta aacaccgtct ggacgttcag
    25981 ttcgacaatg tggaagaagc cattgccatg tcagcaatgt acgcggctaa ccacctgaaa
    26041 ggcgttacgg cgatcatcac catgaccgaa tcgggtcgta ccgcgctgat gacctcccgt
    26101 atcagctctg gtctgccaat tttcgccatg tcacgccatg aacgtacgct gaacctgact
    26161 gctctctatc gcggcgttac gccggtgcac tttgatagcg ctaatgacgg cgtagcggct
    26221 gccagcgaag cggttaatct gctgcgcgat aaaggttact tgatgtctgg tgacctggtg
    26281 attgtcaccc agggcgacgt gatgagtacc gtgggttcta ctaataccac gcgtatttta
    26341 acggtagagt aagtacgttg ccggatgcgg cgaaaacgcc tcatccggcc tacagttcaa
    26401 tgataattca acagatttcg aatcttctga agcaaacttg aacttatcac cagtcgaagg
    26461 cctctcctcg cgagaggcat tttttatttg atgggataaa gatctttgcg cttatacggt
    26521 tggatttcgc ccggtttgcg agttttcagc aattttaata tccaggtgta ttgttctggt
    26581 cgcggaccaa caaaaatctc gacttcttca ttcatccgcc gcgcaatcgt atgatcatcc
    26641 gcctctaaca gatcatccat cggtgggcgc acctgaatcg tcaaacgatg cgttttgcca
    26701 tcataaatcg gaaacagcgg tacaacgcgc gcacggcaca ctttcatcaa acgaccaatc
    26761 gcaggcaacg tcgctttata ggtggcaaag aaatcaacaa attcgctgtg ctctgggcca
    26821 tgatcctgat cgggtaaata atatccccag tagccctgac gtaccgactg gatgaatggt
    26881 ttaataccat catttctcgc atgcagacga ccgccaaagc gacgacgcac cgtgttccag
    26941 acataatcaa aaaccgggtt gccctgatta tggaacatcg ctgccatttt ctgcccttgc
    27001 gaggccatca gcatggcagg aatatcgacg gcccaaccgt gcggcaccag aaaaatcact
    27061 ttctcgttat tacgccgcat ctcttcgata atctccagcc cttgccagtc aacgcgcggc
    27121 tgaattttct ccggcccgcg tattgccaac tcagccatca ttgccatcgc ttgcggcgcg
    27181 gtggcaaaca tctcatctac aatcgcttcg cgttcagctt cactacgttc tggaaagcag
    27241 agcgacagat tgattaacgc acgacggcgt gagctttttc ccagtcgtcc ggcaaaacgt
    27301 cccagccgtg ccagaatggg atcacggaac tttggcggcg ttaaagcgat acccgccatc
    27361 gcggctacgc ccagccatgc tccccagtag cgcgggtggc gaaaggattt atcaaactca
    27421 ggaatgtatt cgctattatt ttttttcgtt tccatgcttt tccagtttcg gataaggcaa
    27481 aaatcaatct ggtgatagtg tagcggcgca acttgccccg caccaaataa aaaaagccgg
    27541 tactgactgc gtaccggctg cgaatggatg ttaattaatc aaaccgtagc tgcggcacaa
    27601 tctctttggc ctgcgccagg aattcgcgac gatcggagcc ggtcagccct tcggtacgcg
    27661 gcagttttgc cgtcagcggg tttacggcct gctggtttat ccatacttca tagtgcagat
    27721 gcggcccggt tgaacgtccg gtattaccgg aaagcgcgat acggtcgcca cgtttcacct
    27781 tctgtcccgg tttcaccagg atcttgcgca agtgcatata acgcgtggtg tagctgcgac
    27841 catgacgaat agccacataa taacctgctg cgccactgcg tttggcaacc accacttcac
    27901 cgtcacccac tgaaagcact ggcgtacctt gtggcatggc aaaatcaaca cctctgtgtg
    27961 gcgcaacgcg accggtcacc ggattagtac gacgcgggtt aaagttagat gagatacgga
    28021 actgtttcgc cgtcgggaat cgcaagaatc ctttcgccag accagtaccg ttacgatcgt
    28081 agaatttgcc atcttcagcg cggattgcgt aataatcttt accttctgaa cgcaaacgta
    28141 cgcccagcag ctggctttgc tcacgtttac catcaagcat ttctcgtgac attaacaccg
    28201 caaattcatc gccttttttc agtttgcgga aatccatttg ccactgcatg gctttaatca
    28261 ctgcgctcac ttcggcgctg gttaaaccgg cgtttctggc gctggcaaca aagcttcccc
    28321 cgacggtacc tttcagcaga ttgttgaccc actctccttg ctgcatttcg ctggtcattt
    28381 taaaaccgtt agcggcagta cggtcatagg ttcgggtttc acgacgagac acttcccagg
    28441 tgaggcgctg cagttcgccg tccgcggtta atgtccagga gagttgttga ccgattttca
    28501 ggttacgcaa ttctttgtcg gcagcagcca gttgggtgat atcacccata tcaataccat
    28561 actgattgag aatgctgctt agcgtatcgc cagtggaaac aacatattca tgcacgcccg
    28621 cttcaccggc gattttgtca tccagttcat cctggggaat ggcttcatct tcttgtgcag
    28681 cttgatcaat cggctcactg gcttcaggta agagcgaacg aatttcgttc tgttccagct
    28741 caatggtttt gacaattggc gtggcatcgc ggtgataaac atagggccgc cagacagcga
    28801 cggccagagt aagaacggtg agcgacccta acataacgcg gtgtggtcgc ggtaaattat
    28861 taaacgccag ggcgacagag cgggctatct gttgcacgta atcacttcct cattaatctc
    28921 ctttcaggca gctcgcatac tggttggcta attgattcag gaattctgaa tagcttgttt
    28981 tacccagttt gatattcgtc cccaggggat ccaacgttcc catacgaacg gatgtccctc
    29041 gtgcgacgct ctcaacgacc gctggcctga actgtggctc agcaaaaacg caggttgctt
    29101 tttgctcaac caactgtgtt cttatttcat gtaaacgctg cgcgccaggt tgaatctcag
    29161 ggttaacggt aaaatgacca agcggtgtca gtccgaactg tttttcgaag tagccgtaag
    29221 catcgtgaaa aacgaaataa cctttcccct tgagcggcgc gagctcgtta ccaacctgcg
    29281 tttcggttga ggctaattgt gcctcaaaat ccttcaggtt ggcgtcaagt ttggctcgac
    29341 tttgcggcat aagttccact aattttccat ggattgcaac cgctgtagcc cgcgctatct
    29401 ctggggaaag ccaaagatgc atgttgaaat cgccgtgatg gtgatcttcg tcactttttt
    29461 ccgcgtggtc gtgatcatca tcatcgtcgt gaatactttt catcagcagc ggtttcacat
    29521 cttctagctg cgcaatcgtt acctgtttcg cttctggtaa tttacttacc ggtttttgca
    29581 tgaacgcttc catctccggg ccaacccaaa cgactaagtc cgcgttctgt aagcgtttta
    29641 catccgatgg acgcagtgaa taatcatgtt ctgaagcgcc gtcaggcagt aaaacctgcg
    29701 tttctgttac cccatcagca atggcagaag cgatgaaccc aacgggttta agcgaagcga
    29761 caacggcggc atctgcggcc tgtgttgcac cgccccagag agcggcggat aatgctgcga
    29821 aaagaagcgt ttttttatgt aacataatgc gaccaatcat cgtaatgaat atgagaactg
    29881 tgatattata acatttcatg actactgcaa gactaaaatt aacatgacaa gtctggtttc
    29941 cctggaaaat gtctcggttt cttttggcca acgccgcgtc ctctctgatg tgtcgctgga
    30001 acttaaacct ggaaaaattt tgactttact tgggccaaac ggcgcaggta agtcgacact
    30061 ggtacgggta gtgctcgggc tggtaacacc cgatgaaggg gttatcaagc gcaacggaaa
    30121 actgcgcatc ggctatgtac cgcagaagct gtatctcgac accacgttgc cactgaccgt
    30181 aaaccgtttt ttacgcttac gccctggcac acataaagaa gatattttgc ctgcactgaa
    30241 acgtgtccag gccgggcatc tgattaacgc accgatgcaa aagctctcgg gtggcgaaac
    30301 gcagcgtgta ctgttagcgc gagcattgtt aaatcgaccg caattattag tgctggatga
    30361 acccactcag ggcgtggatg tgaatggtca ggtggcgtta tatgacctta ttgaccaact
    30421 gcgtcacgaa ctggattgtg gcgttttaat ggtatctcac gatctgcatc tggtaatggc
    30481 aaaaaccgat gaagtgcttt gcctgaatca ccacatttgt tgttccggca caccggaagt
    30541 tgtttccctg catccggagt ttatttctat gtttggtcct cgtggtgctg aacaactggg
    30601 tatctatcgc catcatcata atcatcgtca cgatttacag ggacgaattg ttttgcgtcg
    30661 gggaaatgat cgctcatgat tgaattatta tttcccggtt ggttagccgg gatcatgctc
    30721 gcctgtgccg cgggtccgct gggttcgttt gtagtctggc gtcgtatgtc ttatttcggt
    30781 gatacgctgg ctcatgcctc attacttggc gtcgcgtttg gtttgttgct ggacgtgaat
    30841 ccattctatg cggtgattgc agttacgctg ctgctggcgg gcggtctggt atggctggag
    30901 aagcgtccac agctggcgat cgacacgtta ttagggatta tggcgcacag tgccctgtcg
    30961 ctgggcctgg tggtcgttag tctgatgtct aatattcgtg ttgatttgat ggcttacctg
    31021 ttcggtgatt tactggcagt gacgccagaa gatctcatct ctattgcgat tggcgtggtc
    31081 atcgtggtgg ctattatgtt ctggcaatgg cgcaatttgc tgtcgatgac gattagcccg
    31141 gatctggcgt ttgttgatgg tgtgaaatta cagcgcgtga aattgttgtt gatgctggtg
    31201 acggcattga cgattggtgt agcgatgaaa tttgtcggcg cgttgattat tacttcactg
    31261 ctgattattc ctgctgctac tgcacgtcgc tttgcccgca cgccggaaca gatggctggt
    31321 gtcgctgttt tggtggggat ggtggcagtg actggcggtt taaccttttc cgcattttac
    31381 gatacacctg caggcccgtc ggtggtgcta tgtgcggcac tgttatttat tatcagtatg
    31441 atgaaaaagc aggccagcta atctatcgct gaacacattt gtcggatgcg gcgcgagcgc
    31501 cttatccgac ctatggttcg ctatctctgg tatatgcctg ataagacgcg aatagcggcg
    31561 catcaggcaa cactgccagt gtcggatgcg ccgcgagcga ccgatccgac ttacggcatt
    31621 tctggcggcg tgatgccgaa gtggttccac gcccgcactg tcgccatacg cccgcgcggt
    31681 gtacgctgca aaaagccttg ctgaatcaaa taaggttcca gcacatcctc aatggtttca
    31741 cgttcttcgc caatggcagc cgccaggtta tccagaccta ccggcccacc aaagaactta
    31801 tcgattaccg ccagcaacaa tttgcggtcc atataatcga aaccttcggc atcgacattc
    31861 agcatatcca gcgcctgagc agcgatatca gccgagatgg tgccatcgtg cttcacttcg
    31921 gcgaaatcac gcactcgacg cagcagacgg ttagcaatgc gcggcgtacc gcgagcgcga
    31981 cgagcaactt ccagcgcgcc gtcatcactc atctcaagcc ccataaagcg tgcgctgcga
    32041 ctgacgatat attgcagatc cggcacctga taaaactcca gacgctgcac aataccaaaa
    32101 cgatcgcgca acggtgatgt cagcgaacct gcgcgcgtgg ttgcgccaat cagtgtaaac
    32161 ggcggcaaat caattttaat ggagcgtgcc gccggacctt caccaatcat gatatccagt
    32221 tggtagtctt ccattgccgg atacaacacc tcttccacca ctggtgaaag acggtggatc
    32281 tcatcaataa acagtacatc gtgtggttca aggttagtga gcattgctgc cagatcgccc
    32341 gccttttcca gcaccggacc agaagtcgtg cgtaaattaa cgcccatttc attggcgaca
    32401 atattggcaa gcgtagtttt acccaacccc ggaggaccaa aaatcaacag atgatcgagg
    32461 gcatcgccgc gcagtttcgc tgctttgatg aaaatctcca tctgtgaacg aacctgcggt
    32521 tgaccaacat actcttccag taatttaggg cgaatggcgc gatctgccac atcttccggc
    32581 aaagtggtac cggcagaaat cagacggtct gcttcaatca tcctttacct cataacgcgg
    32641 cgcgtagggc ttcgcgaatt aatgtttcac tgctggcgtc agggcgagcg attttgctca
    32701 ccatacggct tgcttcttgt ggtttatagc ccagcgccac cagcgcagca accgcttcct
    32761 gttcagcatc gtcggtcgcc gggctggcag gtgacgtgag taccaggtcg gcggctggcg
    32821 taaagagatc gccatgcaaa cctttaaatc ggtctttcat ttcgacaatc aagcgttcgg
    32881 cggttttttt gccaataccc ggcagtttca ccagtgcccc cacttcttca cgctcaacgg
    32941 cattaacgaa ctgctgcgct gacattccgg agaggatcgc cagcgccaac ttcgggccga
    33001 cgccgttggt tttgatcaac tctttgaaca acgtgcgctc ttgtttattg ttaaaaccgt
    33061 acagcagttg cgcgtcttca cgcaccacaa agtgggtgaa aacgatcgct tcctgacccg
    33121 cttcagggag ttcataaaaa caggtcatcg gcatatgcac ttcatagcct acgccgccca
    33181 cttcaattaa caccagcggg ggttgttttt caatgatgat gcctctgagt ctgcctatca
    33241 catgacgctc ctgcgtaatg aatcaaagat aatgctgtat gataaaaaaa tgctggatag
    33301 atatccagcg aaggatgaag aaaacttgcg aggtgtcccg atgatctgaa aaatggc
//
LOCUS       DAZGHF010000043        33197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGHF010000043 DAZGHF010000000
VERSION     DAZGHF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.043"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            384..1601
                     /gene="alaA"
                     /locus_tag="ACHK3A_002120"
     CDS             384..1601
                     /gene="alaA"
                     /locus_tag="ACHK3A_002120"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO8740200.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1685..2284
                     /gene="yfbR"
                     /locus_tag="ACHK3A_002121"
     CDS             1685..2284
                     /gene="yfbR"
                     /locus_tag="ACHK3A_002121"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO8740201.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2343..4175)
                     /gene="yfbS"
                     /locus_tag="ACHK3A_002122"
     CDS             complement(2343..4175)
                     /gene="yfbS"
                     /locus_tag="ACHK3A_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO8740202.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4262..4912)
                     /gene="hxpA"
                     /locus_tag="ACHK3A_002123"
     CDS             complement(4262..4912)
                     /gene="hxpA"
                     /locus_tag="ACHK3A_002123"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO8740203.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4923..5417)
                     /gene="yfbU"
                     /locus_tag="ACHK3A_002124"
     CDS             complement(4923..5417)
                     /gene="yfbU"
                     /locus_tag="ACHK3A_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO8740204.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5501..5956)
                     /gene="yfbV"
                     /locus_tag="ACHK3A_002125"
     CDS             complement(5501..5956)
                     /gene="yfbV"
                     /locus_tag="ACHK3A_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO8740205.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6294..7496
                     /gene="ackA"
                     /locus_tag="ACHK3A_002126"
     CDS             6294..7496
                     /gene="ackA"
                     /locus_tag="ACHK3A_002126"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO8740206.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7571..9715
                     /gene="pta"
                     /locus_tag="ACHK3A_002127"
     CDS             7571..9715
                     /gene="pta"
                     /locus_tag="ACHK3A_002127"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO8740207.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9905..11425
                     /gene="yfcC"
                     /locus_tag="ACHK3A_002128"
     CDS             9905..11425
                     /gene="yfcC"
                     /locus_tag="ACHK3A_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO8740208.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11458..12000)
                     /gene="yfcD"
                     /locus_tag="ACHK3A_002129"
     CDS             complement(11458..12000)
                     /gene="yfcD"
                     /locus_tag="ACHK3A_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO8740209.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12058..12609)
                     /gene="yfcE"
                     /locus_tag="ACHK3A_002130"
     CDS             complement(12058..12609)
                     /gene="yfcE"
                     /locus_tag="ACHK3A_002130"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO8740210.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12665..13309)
                     /gene="yfcF"
                     /locus_tag="ACHK3A_002131"
     CDS             complement(12665..13309)
                     /gene="yfcF"
                     /locus_tag="ACHK3A_002131"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO8740211.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERSTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13445..14092
                     /gene="yfcG"
                     /locus_tag="ACHK3A_002132"
     CDS             13445..14092
                     /gene="yfcG"
                     /locus_tag="ACHK3A_002132"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO8740212.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAASQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14149..14511
                     /gene="folX"
                     /locus_tag="ACHK3A_002133"
     CDS             14149..14511
                     /gene="folX"
                     /locus_tag="ACHK3A_002133"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO8740213.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14532..15425
                     /gene="yfcH"
                     /locus_tag="ACHK3A_002134"
     CDS             14532..15425
                     /gene="yfcH"
                     /locus_tag="ACHK3A_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO8740214.1"
                     /translation="MKIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VALWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCAHWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15473..16375)
                     /gene="rpnB"
                     /locus_tag="ACHK3A_002135"
     CDS             complement(15473..16375)
                     /gene="rpnB"
                     /locus_tag="ACHK3A_002135"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO8740215.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLE
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16572..17345)
                     /gene="hisP"
                     /locus_tag="ACHK3A_002136"
     CDS             complement(16572..17345)
                     /gene="hisP"
                     /locus_tag="ACHK3A_002136"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO8740216.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17353..18069)
                     /gene="hisM"
                     /locus_tag="ACHK3A_002137"
     CDS             complement(17353..18069)
                     /gene="hisM"
                     /locus_tag="ACHK3A_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8740217.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18066..18752)
                     /gene="hisQ"
                     /locus_tag="ACHK3A_002138"
     CDS             complement(18066..18752)
                     /gene="hisQ"
                     /locus_tag="ACHK3A_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO8740218.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18842..19624)
                     /gene="hisJ"
                     /locus_tag="ACHK3A_002139"
     CDS             complement(18842..19624)
                     /gene="hisJ"
                     /locus_tag="ACHK3A_002139"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO8740219.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19845..20627)
                     /gene="argT"
                     /locus_tag="ACHK3A_002140"
     CDS             complement(19845..20627)
                     /gene="argT"
                     /locus_tag="ACHK3A_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO8740220.1"
                     /translation="MKKSILALSLLVGLSAVASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20893..21462)
                     /gene="ubiX"
                     /locus_tag="ACHK3A_002141"
     CDS             complement(20893..21462)
                     /gene="ubiX"
                     /locus_tag="ACHK3A_002141"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO8740221.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21557..23074)
                     /gene="purF"
                     /locus_tag="ACHK3A_002142"
     CDS             complement(21557..23074)
                     /gene="purF"
                     /locus_tag="ACHK3A_002142"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO8740222.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23111..23599)
                     /gene="cvpA"
                     /locus_tag="ACHK3A_002143"
     CDS             complement(23111..23599)
                     /gene="cvpA"
                     /locus_tag="ACHK3A_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO8740223.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23858..24508)
                     /gene="dedD"
                     /locus_tag="ACHK3A_002144"
     CDS             complement(23858..24508)
                     /gene="dedD"
                     /locus_tag="ACHK3A_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO8740224.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAVNNTEFEPEPA
                     PVAPPKPKPVESPKPKVEAPPASKPVVEEKAAPTGKAYVVQLGALKNADKVNEIVGKL
                     RGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYTPN"
     gene            complement(24498..25766)
                     /gene="folC"
                     /locus_tag="ACHK3A_002145"
     CDS             complement(24498..25766)
                     /gene="folC"
                     /locus_tag="ACHK3A_002145"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO8740225.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYTSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25836..26750)
                     /gene="accD"
                     /locus_tag="ACHK3A_002146"
     CDS             complement(25836..26750)
                     /gene="accD"
                     /locus_tag="ACHK3A_002146"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO8740226.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26906..27565)
                     /gene="dedA"
                     /locus_tag="ACHK3A_002147"
     CDS             complement(26906..27565)
                     /gene="dedA"
                     /locus_tag="ACHK3A_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO8740227.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27648..28460)
                     /gene="truA"
                     /locus_tag="ACHK3A_002148"
     CDS             complement(27648..28460)
                     /gene="truA"
                     /locus_tag="ACHK3A_002148"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO8740228.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28460..29473)
                     /gene="usg"
                     /locus_tag="ACHK3A_002149"
     CDS             complement(28460..29473)
                     /gene="usg"
                     /locus_tag="ACHK3A_002149"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO8740229.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29539..30696)
                     /gene="pdxB"
                     /locus_tag="ACHK3A_002150"
     CDS             complement(29539..30696)
                     /gene="pdxB"
                     /locus_tag="ACHK3A_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO8740230.1"
                     /translation="MSRTEKLLTILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLAD
                     ADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDDTWLKQAGIGFSAAPGCNAIAV
                     VEYVFSSLLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRG
                     DEGDFRSLDELVQHADILTFHTPLFKDGPYKTRHLADEKLIRSLKPGAILINACRGAV
                     VDNTALLTCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQ
                     VFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLR
                     KVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            complement(30856..31860)
                     /locus_tag="ACHK3A_002151"
     CDS             complement(30856..31860)
                     /locus_tag="ACHK3A_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8740231.1"
                     /translation="MSIKKLEDGRYLLDIRPNGRKGKRVRKVFNKKSVAVATERYIMA
                     NAEKREYIQGYRDRRTLNDLLELWWVYHGQHRRKAEEDRKQLRNIINELGADMQAVDL
                     DKLKIIAWRSRRIADGLKPSSANRYMNRLSGMFTALKKIGLWDAEHPVRGISVLYVSP
                     REMAFLSQKEVALLLDTLEGDYWRVALLCLSTGARWGEASKLRGEQIVHNRVTFLETK
                     NGRKRTVPISQAVCEAIKTSETGGLFDVKYREFCLVLKRVKPDLPKGQAAHVLRHTFA
                     SHFVMNGGNIIALQRILGHATIQQTMAYAHFAPDYLQDAVALNPLKGGVSVHSVSTGE
                     "
     gene            complement(31957..32286)
                     /locus_tag="ACHK3A_002152"
     CDS             complement(31957..32286)
                     /locus_tag="ACHK3A_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8740232.1"
                     /translation="MPDMASLSSMGEKFRLIREAEGMTRQEFADCVGLPYGTVTNYEV
                     RGKQVTEGALLKVTKHPKFKKYAYWLSTDETMPEAGQIAPALSLDGSCNLEGNQDSTE
                     TTRKLPR"
     gene            32391..32678
                     /locus_tag="ACHK3A_002153"
     CDS             32391..32678
                     /locus_tag="ACHK3A_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO8740233.1"
                     /translation="MRESELEGFIQVAPYPLEAVPYQLFAKMIGRKESTVRTMIDAAK
                     LPTIDFVKPGSVKTRASENWVYLPAFNAGMRKAFFNQPKEQRDAWLLWLGL"
     gene            32874..>33197
                     /locus_tag="ACHK3A_002154"
     CDS             32874..>33197
                     /locus_tag="ACHK3A_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO8740234.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNTHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHP"
BASE COUNT         8316 a         9038 c         7995 g         7848 t
ORIGIN      
        1 cccgcttaac aatagtttca caccttgcac ccattacgac acctcggaaa tcatcacgct
       61 gttaaactct ccgtaatgcg gaaatatcat catcacgatt cattagtgaa ttcttaaacg
      121 aggcattttc aaacaattat cttacagata aaaaaaccac agtcacaatt aagaatcaga
      181 acaattcact acataacatt aattactaag catatacacc tcattatttt gccattatta
      241 agttattaac agcacaatcg agtcttcccc tctggcaaaa tcttattctg cagaccttca
      301 aaacgccgtc ctgggggagt acattgttct aagctgactt ccacggcagg gagtggcgat
      361 aacagcaaaa aaggtcaaga ttcatgtccc ccattgaaaa atccagcaaa ttagagaatg
      421 tctgttacga catccgtggt ccggtgctga aagaagcaaa acgcctggaa gaagaaggta
      481 acaaggtact gaaactgaac atcggcaacc ctgccccgtt cggttttgac gcgccagatg
      541 aaatcctcgt tgacgtgatt cgcaacctgc ctaccgctca agggtattgc gattccaaag
      601 ggctttactc cgcgcgtaaa gccatcatgc agcactacca ggctcgtggc atgcgtgatg
      661 ttaccgtgga agatatttac atcggaaatg gtgtatcgga gcttatcgtt caggcaatgc
      721 aggcattgct gaacagcggc gacgaaatgt tggttcctgc acctgattac ccactatgga
      781 ccgcggcggt ttcgctctcc agcggtaaag cggtgcatta tctttgcgat gaatcctctg
      841 actggttccc ggaccttgat gatattcgcg ctaaaattac gcctcgtacg cgtgggatcg
      901 ttattatcaa cccaaataac ccaaccggcg cggtatattc caaagagctt ttaatggaga
      961 tagtggagat tgcacgtcag cataatctca ttatcttcgc cgatgaaatt tatgacaaaa
     1021 ttctctacga cgacgctgag catcactcaa ttgcgccgct ggcacctgat ctgctgacca
     1081 ttacctttaa cggcctgtcg aaaacatacc gcgttgcagg cttccgtcag gggtggatgg
     1141 tgttgaacgg gccgaaaaaa cacgccaaag gctacatcga aggtctggaa atgctggctt
     1201 caatgcgcct gtgtgctaac gttcctgcgc aacacgccat tcagaccgcg ctgggcggtt
     1261 atcagagcat cagtgaattt attacccctg gcggtcgtct ttatgagcag cgtaaccgcg
     1321 catgggaatt gatcaatgat attccgggcg tttcctgcgt gaaacctcgt ggtgcgctgt
     1381 acatgttccc gaaaatcgac gccaaacgct ttaatattca cgacgatcag aaaatggtgc
     1441 tggatttcct gttgcaggaa aaagttctgt tggtgcaagg gacggcattc aactggccgt
     1501 ggccggatca cttccgcatt gtcacgctac cgcgtgtcga tgatatcgag ctgtctttga
     1561 gcaagttcgc gcgtttcctt tctggttatc atcagctgta atcttaattt cactgccgga
     1621 gattgcatcc ggcagtgtta tcccgccaca atgacctgat gatgtcatca tacgtaaggt
     1681 cactatgaaa cagagccatt tttttgccca tctctcccgc ctgaaactca ttaaccgctg
     1741 gccgctaatg cgcaatgtgc ggacggaaaa tgtgtccgaa cacagtttgc aggtagcgat
     1801 ggtcgcccat gcgctggcag ctatcaaaaa tcgtaaattt ggcggtaatg tcaacgccga
     1861 acgtatcgct ttactggcga tgtaccacga tgcctcagaa gtgcttaccg gcgatctccc
     1921 taccccggtg aaatacttca attcgcaaat cgctcaggaa tacaaggcga ttgaaaaaat
     1981 cgctcagcaa aaactggtcg atatggttcc ggaagagttg cgggatatct ttgcgccgtt
     2041 aattgacgag catgcatata gtgatgaaga aaaatcactg gtgaaacagg cagatgcact
     2101 atgtgcatat ctgaaatgtc tggaagaact cgcggcggga aataatgaat tcttgctggc
     2161 aaaaacgcga ctggaagcga cgcttgaagc gcgccgcagc caggagatgg actacttcat
     2221 ggaagtcttt gttcccagct tccatctttc gctcgatgag attagccagg attcaccgct
     2281 gtaagcagcc ggagtctgcg tcgcatcagg caataagcgc cggatgcgac atcaggctct
     2341 tgtcaaaacg gaaacagcat cgggatcatc accacacaaa ccgccatcac gataatggtg
     2401 aacggtaccc ccaacttcac aaagtcactg aagctgtaat ttcccggacc taaaaccagt
     2461 gtgttaacag gtgaagaaac cggcgtcata aaggcggcgg atgctgccat cgccacgacc
     2521 atcgcgaatg ggtaaggcga cacccccatc gttttagcag cagccagcgc aatcggagcc
     2581 atcaacaccg ccgtcgcggt attagaaata aatagcccaa taaccgccga caagacaaac
     2641 aaacaaccca gcatcatatg tggcccgtaa ccgccgccaa tgtccatcag ccctttcacc
     2701 gccagcgcaa cacctcccgt tttctgtaat gccacagcaa acggcatcat cccaacgatc
     2761 aaaataatgc tcggccagtg tatggattta taggcgcttt cagcatcgat acagcggaat
     2821 ttccccatca gcaggcaggc gatgatagcg gcaacaggat taggaatttc atcggtcagc
     2881 attaacgcca ccattagcac cagacagaaa atggcatggg gtgcctgact gtgcgcgggc
     2941 gatgcttcac tcacctcttc cggtaagttc agcgctacga agtcgcggcc ctgtttggcc
     3001 agcataccga tcagtttcca gttacccaca accaggatga tatcgcccag cagcagaggc
     3061 tcatccgcca gcgaaccttc cagcgccacg ccattgcgct ttagccccac tacattcaat
     3121 ccgtagcggg tacgaaaacc aatttcgcgc accgatttac caataagttc tgactcagga
     3181 attaatgaaa tctctgccat gcccacatca agggcctggt cagaaaaata ctcgccgcgc
     3241 agtaccatcg gctccagcaa ttgctcacta caaaattgcc ggagatcgac atcagccgca
     3301 gacatatcaa taagcaaaac gtcacgcgcg cgaaattcag aaaccccatt aacgttcacg
     3361 ataacgcgac gaaaacgccg ccagcgttca acaccgatga cgttagcacc ataacgttca
     3421 cgtaatttga gatcgtccag tcgttgacca atcattggcg atccggggcg aatagccaga
     3481 cgtcgcgcac gcccggtcag tcgatattca cggataagat cgcgaaaggt tcggcgcgtc
     3541 cagccttcgc gttgtggggt ctgggtatcc cctttcagca tgaaacgcat cactaacata
     3601 tacaaaatac ccagcaccag cacgaccagg ccaataggtg ttacgctaaa gaaactgaag
     3661 ccgtgatagc cttcacgcag caattcactg ttgactacca ggttcggcgg cgtcgccacc
     3721 agcgtcatca tgccgcttat cagcccggca aaacttaacg gcatcatcag acgcgacggc
     3781 gacgtttgca tacgcatggc aacgcttaac accacgggaa taaagatagc gacaacgccg
     3841 gttgaactca taaacgcgcc aagccccgcg acggtcagca tcaacaaaac caacattttg
     3901 atttcactgt tgcccgccac tttgaccagc catgtcccca ttacagtggc aacaccggta
     3961 cggaccaaac catcgccaat aatgaacaag gcggcaatca ggacaacgtt aggatcggaa
     4021 aagccggaaa atacttcggg gaccgtcagc gttccgctta atgcaaacgc gacaataaca
     4081 aacaaagcga ccgcatccat acgcactctg cccgtcgcaa acaagacgat ggcaacggcc
     4141 agtaatgaaa gaacccaaat caattcaccg ttcacaactt atccttgtta attgaggggg
     4201 ataacttgat tctgccataa aaaagccccg acgagacggg gctaaatcat gatcaggtgt
     4261 ttcactgaat aataacatcg ccatttggct gtttggtcac agtaatttgc tccagactgt
     4321 ggaggaccaa atcgacctca ttcaggcgcg gggtatctgc cggagcgtta accgcaatga
     4381 catggcaacc cgccgccagg ccagaaagca cgccagcggg agcatcttcc accactacac
     4441 actcctgcgg cgcaagcccc agcagctgcg cgcctagcag atacgcatca ggttctggtt
     4501 ttccgcgctt cactcgctca gcggtcacaa acacctctgg cgcgggaagc ccagctattt
     4561 tatggcgcgc tcgcgctacc ggcatggagc cagaagtcac aatggcccac ggaatacctg
     4621 ctttattcaa atgattgagt aaggcgattg cccccggaag tgcggtaata ccctcggttt
     4681 ccgtggcctc gatttgctcc agacgcgtaa actcggcggc aatatcagcc tcggatttgc
     4741 ccgccataaa atggcgcaaa gaggtgatcg cctgtttacc gtgaataaaa gccagcacct
     4801 cttccggcgc taacccatga cgtctggccc agttgctcca cgcccgttct accgcaggca
     4861 gggaatccac cagcgttcca tcaagatcaa acagaaaacc tttgcaccgc acgcgggcct
     4921 cctcaggcgt tgataatttg attaatttcg ttggcgctca aatggtactg acgcgggcag
     4981 gcatgccaca cattaagcat gcgctgatat ttttcccaca ttggcgtctg ggcgttaaaa
     5041 ccgtgagttc cggcatcaaa atgggtatag cgtccttcca cattaaccat aaagcggaca
     5101 taaccgaggt aacgtgcttc agtggcggcg tcaaagccga ggaaggtgac acgacgttca
     5161 tcgatggatt gctggtcctg caaattagac caggaaacat gcaacgcatg atacatctcc
     5221 ataatgtcga tgatagtgcg gcaggtttct tctttcagct cgccaaactc gcgatccagt
     5281 tcacgcatct gtaatccata accacgctca atgattgttt gcaggcgacg gtaacgttca
     5341 gcatttgccg gatcgagcat agtcatcatc ttgtactggt tagacaaaat aagacgttgc
     5401 gcgttggtca tttccattgt tgactcctgt atcactctac tacggtgaaa aaaaaagaag
     5461 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
     5521 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
     5581 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
     5641 accagttaag gattgcaggg ggtaatggcg ttacagaacg tttgcccagc caccacaatc
     5701 cctgcatggg taaacttaag gcgaacagcg cagtggcaac tgccggccca agctgaccgc
     5761 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
     5821 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagcg
     5881 gccacgtctt tgagtaatgc tgtccccggc gaaacaagct aaaaaaatta acagaacgat
     5941 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
     6001 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
     6061 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
     6121 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
     6181 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
     6241 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
     6301 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
     6361 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cccgaagcac
     6421 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
     6481 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
     6541 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
     6601 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
     6661 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
     6721 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
     6781 tctacgccct gccgtacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
     6841 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
     6901 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
     6961 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
     7021 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
     7081 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
     7141 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
     7201 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
     7261 gtcgtctgga cgctgttgta ttcaccggtg gtatcggtga aaatgccgcg atggttcgtg
     7321 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
     7381 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
     7441 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
     7501 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
     7561 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
     7621 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
     7681 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
     7741 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
     7801 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
     7861 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
     7921 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
     7981 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtattgaac tgacccgcaa
     8041 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaatgc
     8101 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
     8161 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc cgctgccggt
     8221 tctcggcgcg gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
     8281 ccacctgaat gcaaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
     8341 cactttctgc gcacgcagca ttccacacat gctggagcac ttccgtgccg gttctctgct
     8401 ggtgacttcc gcagaccgtc ctgacgtgct ggttgccgcg tgcctggctg ccatgaacgg
     8461 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
     8521 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
     8581 gcagacttct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgagcg
     8641 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgattctct
     8701 gactgccact tctgagcgca gccgtcgtct gtctccgcca gcgttccgtt atcagctgac
     8761 tgaacttgcg cgcaaagcgg gcaaacgtat cgttctgccg gaaggtgacg aaccgcgtac
     8821 cgttaaggca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
     8881 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg caggcattga
     8941 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg agctgcgtaa
     9001 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagataacg tggttctcgg
     9061 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
     9121 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
     9181 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
     9241 caacccggat ccgaccgcag aacagctggc agaaatcgcg attcagtccg cagattccgc
     9301 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg ggacttctgg
     9361 tgctggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
     9421 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
     9481 atccaaagca ccgaactctc cggttgcagg tcgcgctacc gtgttcatct tcccggatct
     9541 gaacaccggt aacaccacct acaaagcggt acagcgttct gctgacctga tctctatcgg
     9601 accgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
     9661 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaatctcg
     9721 tcatcatccg cagctttacg ctgcggatat ctgaaccgga aatagtcact atttccggtt
     9781 ttttattctc ttaatttgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
     9841 atgaattgcg ccatctcaat ttgcatactt accactttgt tttgtgcaag ggaatatttg
     9901 cgctatgtcc gcaatcactg aatctaaacc aacaagaaga tgggcaatgc ccgatacgtt
     9961 ggtgattatc ttttttgttg ccattttaac cagccttgcc acctgggtag ttccggtggg
    10021 catgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    10081 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    10141 cgtacagctg ttcacgacgg gcgatgaacg cccgggtctg atgaacttcc cgtttgaagg
    10201 gttaacctca ggatcgaaat acgggacagc cgttggcatc atcatgttta tgctggtgat
    10261 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    10321 tattcgccat acccgcggaa atgaaattct ctttattcct gcgctgttta ttctgttttc
    10381 acttggcggc gcaatatttg gtatgggaga agaggccgtc gcctttgcca ttattatcgc
    10441 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatatcgc
    10501 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggttg ttgctcaggg
    10561 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    10621 caccctgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    10681 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    10741 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
    10801 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    10861 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    10921 taataccatg gcttcatcct ttaccgaagg ggcgcgaatg atgatcgccc ctgccctgct
    10981 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
    11041 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg ataacgcggt
    11101 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga catctggttc
    11161 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgtaaa
    11221 ccgtcaggtt accgttctgg cgttccagtt tggtgatggc ttcagccaca tcatttaccc
    11281 gacctcagct tcgttaatgg caacgctcgg tgtttgccgg gtggacttcc gtaactggct
    11341 gaaggtgggc gcgacactgc ttggactgct gtttattatg tccagcgtcg tggtgatcgg
    11401 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    11461 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    11521 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    11581 ccagcaaact tcgctgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct
    11641 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    11701 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    11761 ttgctcatcg gcctggacta caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    11821 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    11881 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    11941 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    12001 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    12061 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    12121 gtcattatcc agcatgccat aactcgccgg attaccgcct ttaggaatac tcaccgagcc
    12181 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    12241 caccagcaca tcgttctggt ttaaagcagg taggttttcc gggccaaaca gatgaccatg
    12301 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    12361 atgcaaaagc atttgatcca cttcgctgtc gcaattgccg cgcacagcga taaccttatg
    12421 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    12481 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cactttgggc
    12541 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    12601 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    12661 gctatcagcc agattgcttc gccgaaagag caataaaacg ctggacagac gctcgctgcc
    12721 actggaatgt tgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    12781 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    12841 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    12901 ccgccgttaa tggcgctttt ttcgcacccg caaaaacgac atctgtcgaa cgttcttcgc
    12961 ggatgggcat cagatcgcta cgcagccagg cctgaatctg tcgtgcacgc gcacgatttt
    13021 ctaaatcaag cggataaatg cgttcccagg tcggtggcgc aaatcgatct tccagatact
    13081 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    13141 gtgtctgacc gtaaccttgc cacgtcggct gcaaatgttc accgctgtcg aggtcgatgg
    13201 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    13261 gggagaaaaa atgggcatct gaccaaagcg tgatagcggg tttactcatt atgacctcgg
    13321 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    13381 ctcacgcaag tcgccgcgct ttctatactt gtggtgttca gatgattaga caaccggagc
    13441 tgcaatgatc gatctctatt tcgccccgac accaaatggt cacaaaatta cgctgtttct
    13501 cgaagaagca gggctggatt atcgcttgat aaaggtagac ctggggaaag gcggtcagtt
    13561 tcgcccggaa tttttgcaca tttcgcctaa caacaaaatt ccggcaattg ttgatcattc
    13621 tccagccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    13681 ggctgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    13741 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    13801 tcacgcagcc tcccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    13861 tctgtaccat gtactgaaca agcggctgga aaactcgccc tggctgggtg gcgagaacta
    13921 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    13981 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    14041 cggtcaggca ctgttaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    14101 ggttctcgtg tattatttca tcctaagaaa aacaacggag aacctgcaat ggcacaacct
    14161 gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa
    14221 gaaattaaca accgtcagga tattgttatc aatgtgacga ttcactaccc cgccgataaa
    14281 gcgcgcacca gcgaagatat caacgatgcg ctgaattatc gcaccgtgac gaaaaacatt
    14341 attcagcatg tagagaataa ccgtttctct ttgctggaaa aattaactca ggatgtgctc
    14401 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    14461 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat
    14521 cgggaggctg catgaagata gtgatcaccg gagggacagg attaattggt cgccatttga
    14581 ttccacgttt gctggagctg ggccatcaaa tcacggtaat gacgcgtaac ccgcagaaag
    14641 ccagttctgt tctcggccct cgggtggcac tatggcaagg gcttgccgat caaagcaacc
    14701 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    14761 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    14821 atttgattaa tgccagcgac acgccgccgt cggtactcat ttccggctcg gcaacgggct
    14881 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    14941 ctcataaact ctgcgcccac tgggaagaaa ttgcctgccg ggcgcaaagt gacaaaacgc
    15001 gagtgtgcct gctgcgtacc ggcgtagtgc tggcaccgga tggcggtatt ctcggtaaaa
    15061 tgctgccgcc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    15121 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgagctgc
    15181 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    15241 tcggtcatgc tctgcatcgc ccggctattt tgcgcgtccc cgcaaccgcc attcggctgt
    15301 taatgggcga atcttcagta ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    15361 aagaagcggg ttttgcgttt cgttggtacg atttagaaga ggcgctggcg gatgtcgttc
    15421 gctgatgtgg tttactgcaa acatccgcca gttaactccc ggtgttacag ggttagtggc
    15481 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctcggtcta
    15541 aacctctctc cagcatctcc tgagcaatac gcagggcttc ttcgcgtttg ccaagaatta
    15601 atccatcatt ccgcccttct tcacgtaatc tgtcagcaat agtcatcagt ttctcctttt
    15661 cttgtggtgc gcgttccgct atctcaccaa taaatgcacg gaaacgctgg gcatcccccg
    15721 tttgtaatac gtaattaaac agggctttta gctgtctgtc attagtgttc cctgtaacta
    15781 gcagcgaaac aatttggtcg actaatccca acagatcgcg ctgacgaata tgtttctgaa
    15841 ttaactccaa cagcgccatt ttgcgatgtt gcataatctc gtcatccggc accacggtaa
    15901 tatctaccag cggaaaagcc gatgaataaa ttttgcgggc tatagcaggc tcggcaaatt
    15961 catcaagcca gcaaagtgaa taaggataag gacttctgca accatgataa aacagcatcg
    16021 ggatcaccaa tggaagctct ttatagcctg catcaagatg gttttgcatc gccgcaatgg
    16081 aatagcgcat catgcgaaaa gccattaatt cttccggctt actttggtgc tctatcacta
    16141 cataaatata acctgctccc tcctgcgttt tcacagacca caagaggtcg gaataatatt
    16201 gccgcaggtc ttcatcaata aaactgtttg gttccagttt tagcgttgtt aaatcacaca
    16261 gtttgcgcag cggcgcggga agatgaatat caataaaatc ccgcgcggtg tctggatggc
    16321 gtaaaaaaga tttaaatacc gcatcatgcg gcgtggaagt tgtcgatatt gtcatggcgt
    16381 tccgtcaccc ctcaaattta agatgacgcg acaataacca cgattaatac ccaatgcagc
    16441 aggcagtttt tatctttgga cagcgaattc agaactattt ttagcggact gcaactttta
    16501 catttcagcg gaagctgact cgcaaattcc ccttgaaaat actccggcag cgttagccac
    16561 cggaggggga attatttcag cgatccctta aggaactgtt gcagacgagg gctttgcggg
    16621 ttaccaaata actgttctgg cgcgccctct tcttctattt tcccctgatg gaggaaaatg
    16681 acgtgagtag aaacatggcg agcaaagccc atttcgtgag tcactaccac catggttttc
    16741 ccctcttctg ccagttgctg cataatacgc aacacttcgc ctaccagttc aggatcgagc
    16801 gccgaggtag gttcatcaaa cagtaaaact tccggttcca tcgccagcgc ccgcgcgata
    16861 gaaacacgct gttgctgacc accggacagg tgcaccgggt atttcccctg cgcacgttcg
    16921 tctatcccga cttttgccag atatttcacc gcccgctcgc gcgcttcctg cttgctcagg
    16981 cccaacacct gaatcggcgc ttccatgacg ttttccagca ccgtcatatg gctccagaga
    17041 ttgaagtgct ggaataccat cgtcaggcgt gtgcgcagta agcgcagctg atttttatcg
    17101 gcgactttga gttgaccatc tttgtcgcgc accagattga tcgtctggcc attgaccacg
    17161 atcgatcctt cgctcggttt ttcgaggaag ttaatgcagc gcagaaaggt acttttcccc
    17221 gatcccgacg atccgatgat gctaattaca tctccggcat tcgcttgcag tgaaacccct
    17281 ttcagcactt cgtgttcgcc gtagcgttta tgcaaatcga taacgtttaa tttattctcg
    17341 gacatcgtat tctcagtgcg ttgaagaagg tttcacatgc tgcaaccagc gtttttccgc
    17401 tctgcgaaag aggctgatca ggacataaga gatgattaaa tagagcaccg cggcaatgcc
    17461 gaaggcggta aaaggttgat atgtggcggc gttaatatcg cgggcaattt tcagcagatc
    17521 cggcaccgtg gcagtaaatg ccagcgcagt agagtgcagc atcaggatca cttcgttgct
    17581 gtacgccggt aacgcaatac gcagcgcaga aggcaaaata atgcagcgat acattttaaa
    17641 ggtcgagaag ccataggctc tggcggcttc aatttcccca tgcggcactg aacggattgc
    17701 cccggcaaaa atctcggtag tgtaagcgca ggtgttaagc gtcagcgcca gcacggtaca
    17761 gttcagacca ctgcgaaaaa aggcgttaag gaactcggtt cccttaacaa tctccagcgt
    17821 gtacatgccg gaatagaaca ccagcaactg aacatacagc ggcgtaccgc gaaaaatata
    17881 ggtaaataac cagattggaa actggatgta tttattactt gagacgcgac caatcgccag
    17941 aaacagcgcc agaactccgc ctatcactac cgacaaaata agcagccaca gggtgatcgc
    18001 cacgccagta aagcgataac cgtcggtcca cagcagtggt ttccagtatt catgtaagat
    18061 ttcgatcaca ggtcagccct cttcacaccc acggagtagc ggcgctcaag gaacagcagc
    18121 acaccattgg aaacagtggt gaaaaccagg taaatcacgc cacagacgat ggcgaaatag
    18181 aacggttccc aggtactttt gcctgccagc tgagtggctt tgaccacatc ttccaggccg
    18241 agtaacgaaa cgagtgcggt agatttgagg atcacctgcc agttgttgcc aatgcctggc
    18301 agggcgtaac gcatcatcgc tggaaacatg atccgtcgaa acacttgccc acgagtaaaa
    18361 ccgaacgccg tcgccgcctc tatgtgtcct ttcggcactg ccataaacgc gccacgaaac
    18421 gtttcggtaa aataagcacc gtagataaaa ccgagagtga taatacctgc gaccatcgga
    18481 tcaatatcaa tctgcccgac gcccatcgcc tccgtcaccg tgtttagcgc aatctgcaaa
    18541 ccgtagaaaa tcagcagcat caacaccaag tccggcacgc ctcgaatcag cgtggtgtac
    18601 ccttcgaaaa tcagccccga aagccgattt tgcgagagct taccgccagc gccaattaaa
    18661 ccgataatta cagcgagcac tacagagctg atagccagct ccagcgtgac gagcgcaccc
    18721 tgtaaaataa cacctgaaaa cccatacaac atgctgcctg tcctgtcgtg tgtggtgaat
    18781 taccgcctct tgtctccctc cggttacccg aagggagcgg actttctttc accgatggcg
    18841 attaaccacc ataaacatca aaatcgaagt actttttcgc taatttctcg taagtaccgt
    18901 cagcgcgcat ttcggcaaag gctttgttca gtgcctcgcg cagttcgtta tcttctttac
    18961 gcaggcccat gccggtccct acgccaaaca gtttttcatc tttaacagac ggaccaccga
    19021 atttgtaatc tttaccgacg ggttgtttga ggaaaccttc gctggcagcg acctcatcct
    19081 ggaacgcggc atcaatacgt ccggcagtca ggtcagaata aatgttgtcc tgcccctgat
    19141 aagaaacgat ttcaatgcct tttggtgccc aatgttcgtt accgaacgtc tcctgggtgg
    19201 tgccctgcaa tacgcctacc cgtttgcctt tcagcgactc gactgtcggc tgaatgtcgg
    19261 aatttttcgc caccaccaga cgagaatcgg cagcgtacag tttgtcggtg aaggcaattt
    19321 cttgctgacg tttttccgta atggaaagcg atgacatgat ggcgtcaatc ttcttcgctt
    19381 ttaaggacgg gattaacgca tccagtgggt tttcgacaaa cgtacattgc gtattgatgc
    19441 gtttgcacaa ctccttcgcc agatcgatat cgaagccaac cagttcgcct tgtgaatttt
    19501 ttgattcaaa tggcgcatag gtcggatcgg taccgatgcg gatgttttgc ggaatcgcag
    19561 caaacgccgc agttgcgctg gagaaggcca gaaccagaga gagcgatagc accagttttt
    19621 tcataatgtc ctcaactgac agtcttttta tagggaatat ttacaggttt gttacactta
    19681 tcgtgccata aaattgacca tcaaggcaaa atatcccgcc ccgttagacg tattattttt
    19741 cattaaaaat gcttaagtat gcatttcaat gcaccacgat agtgcaacaa acctggcgtg
    19801 caccatgatg atcattccat caggtacagt ttccctgcga cgtatcagtc accgtagaca
    19861 ttaaagtcga aatacttttt cgccatcttg tcgtaggtac cgtcctgacg cagctcgcca
    19921 agcgccttat taaacgccgc cgtcagttca gcatcatctt tacgtagccc tacaccggta
    19981 ccgtcaccga agtatttttt gtctttcact gatgagccag caaaggcgaa atctttacca
    20041 gcaggttgct tgaggaaacc ttcgctggcc gccacttcat cctgcagtgc ggcatccaga
    20101 cgtcctgcag ccagatcgga atagaccaaa tcctggttgg cataggccac cacatccaca
    20161 cctttgctac gccaggtctc gttagcgtaa gcttcctggg ttgatccctg cagcacaccc
    20221 acatgtttac ctttcagtga atccagcgtt ggctgaatcg gcgaaccttt ggcggcaatc
    20281 aaacgagaat ctgcggcgta caacttgtcg gagaaagcaa tctcctgctg acgtttatcg
    20341 gtaatggaga gcgacgaaat aatggcgtcg atttttttcg ctttcagtga ggggatcagc
    20401 gcgtcaaagt cactggcaac ccaggtacat ttcacctgca tccgtttgca catctcgtta
    20461 ccgagatcga tatcaaagcc aacaaagtca cctttggcat ctttcgatga gaacggcgca
    20521 taggtggtat cggttccgat acgtaccgtc tccggtagcg ccgcatagct ggaagccact
    20581 gcggagagac cgactaacaa agacagagcg agaatcgact tcttcataca taaccctcaa
    20641 gtgaaaatgg cttttttatg ttgtgtattg tgttgtgtgt ttgcaggctc tttcatgcag
    20701 gtcttatgcc atctttacga caacagtaac attcaacgtt aaatatgtta ataagacgtt
    20761 gcattattgt cctaaagttg aagatagcag gtatggcggt tgaatcgcag cgtttcgatt
    20821 catcgaaagg aacaaaatgt cgaggatttg atcgcggttg caaaattgcc ctgaaacagg
    20881 gcaacaatga ggttatgcgc cctgccagcg ggcaaagaga tcttcaggaa gggttatcgc
    20941 aaactggtca agaacacgat taaccgtctg atttatcaca tcatcaaggg attgtgggcg
    21001 atgataaaac gccggaacgg gaggcataat caccgcaccg atttctgccg cctgagtcat
    21061 taaacgcaga tggcctaagt gcagtggcgt ttcacgcacg cagagcacca acgggcgacg
    21121 ctctttcagc accacatctg ccgcacgggt cagtaagcca tcagtatagc tatggacaat
    21181 gccggacagg gttttgattg aacagggtaa aatcaccatc cccaacgtct ggaaagaacc
    21241 ggaagaaatg ctggcagcaa tatcgcgcgc atcgtgtgtc acatcagcta acgcctgcac
    21301 ttcgcgcagg gaaaaatccg tttcgaggga taaggtctgg cgcgctgcct ggctcattac
    21361 cagatgcgtt tcgatatctg tgacatcgcg cagaacctgt aataaacgca cgccataaat
    21421 cgcgccgctg gcaccgctga tgcctacaat gagtcgtttc ataaatagtt gccctgtcag
    21481 acttgcaggc agactttgca ggatttcgac gggagttgca agtcagggtg ccagaccggc
    21541 accctgagcg aaggcatcat ccttcgttat gcatttcgag attttccact tcgttctgac
    21601 gttgcactgc tttggcgtcg tcattacgta aagtatcgag gaaatcgagg tagccctgat
    21661 caacgtcttt ggtgacgtag acgccgttga ataccgagca ttcaaactgc tggatatccg
    21721 ggttttcagc gcgaacggct tcgatcagat cgttcagatc ctggaaaatc aacccgtcag
    21781 caccgatgat ctggcgaatt tcatctactt cgcgaccgtg agcgatcagt tccgtggcgc
    21841 tcggcatatc gataccgtaa acgttcggga agcgaatttc cggtgccgca gaagcgaggt
    21901 acactttctt cgctccggct tcgcgtgcca tctcgataat ctgctcagaa gtggtgccac
    21961 ggacaatgga gtcgtcgacc agcaagacgt ttttatcgcg gaactcggcg cggttggcgt
    22021 tcagtttacg gcgcacggac ttacgacgca gctgctggcc cggcatgatg aaggtgcggc
    22081 caacatagcg gtttttaacg aagccctggc ggtacggttt gcccagaata cgagcaattt
    22141 ccagcgcgat atcacacgag gtttccggaa tagggatcac cacgtcgata tccagatctt
    22201 cccattcgcg ggcaattttc tcgcccagtt tggtgcccat attcacgcgc gcgctgtaaa
    22261 cggaaatttt gtcgatgaac gagtccgggc gggcaaagta tacatactca aacaggcacg
    22321 gattgctgac cggattgtca gcacattgac gggtaaacaa ctgcccttct tcagtgatgt
    22381 aaatcgcttc gcccggcgcg acgtcacgca ggaaatcaaa gcccagcgta tcgagcgcaa
    22441 cgctttcgga agcgaccata tattctgtac ggttctcgtc aatatcacgt tttcccagta
    22501 ccaacggacg aataccgttt ggatcgcgga aagcaaccat accgtggccg atgatcatcg
    22561 ccacacaggc atacgcgccg cggattaagc ggtttgtagc agcgatggcg gcgaaaatgt
    22621 tgtcggcttc cagcggatag tggcggaagt tgtccagttc gctggcaaag atattaagca
    22681 gaatttccga gtcggaagtg gtgttgatgt ggcggcgttt ttcttcaaac agttttttac
    22741 gcaactcgtg agcgttggtc agattgccgt tgtgggcaag cgtaatgcca tacggggagt
    22801 taacgtaaaa cggctgcgct tcagaggcgc tggagctgcc agccgtaggg taacgcacat
    22861 gaccaatgcc catattgccc tgcaaacgct gcatatggcg agcttcaaat acatcgctca
    22921 ccagcccgtt cgctttacgc aaacggaagc agttattggc atctatggtg atgatgccgg
    22981 cggcatcctg accgcgatgc tgaagcaccg ttaaggcatc ataaatcgac tggttaaccg
    23041 gcataacacc ggcgataccg acaataccgc acatacgtct ttttcctcgt taagccacat
    23101 ctcagagcac ttacgctctg ggcaagaaac ttgacgagct ttgcagataa tcaaaaaacc
    23161 atctgatgat aaaactgaac tgcgggatca gctgtgattt gctccagtct tcgcttttcg
    23221 acaccccggt aaaggagtcg agaaagaaga gaatggcggc aacaatcaac acaccgcgca
    23281 acgcaccgaa acagacgccc agcacccgat cggtgcctga caaccccgtt ttctctacca
    23341 actggcctat cacgaagttc acgatagcac caacgatcag ggtagcgata aacagtaccg
    23401 cgatggcaat cccatttcga accagttcgt cttcaaagcc cgtaaaccag actgacaggt
    23461 aagtgtagta atgactggca acaaagaaag cacaacccca tgtcaccagc gataacgctt
    23521 cacgaacaaa gccgcggatc aggctaacca gagaggaaaa agcaatcacc gcgattatgg
    23581 cgtaatcaat ccagaccata tgtgtcccac gattttacgc cctgtcatcc tgttcggggc
    23641 gcattctaac agaaaaagaa aacgtttgcg tagggatttc cttcccgcgc atcaataaaa
    23701 atggcgctga aaaaacattc aacgccatcg actttttatg cctttgcggc atcgggcaat
    23761 gcgtgtcgga tgcggcgtga acaccttatc cgacctacgg ttctacccct gtgtaggcct
    23821 gataagacgc gccagcgtcg catcaggcaa gaccgtatta attcggcgta tagcccatta
    23881 ccacgccact taagccagaa agttgcttca actcacccag cgatcctttc agcttatctt
    23941 tcgaggcatc aggcccaacc agaatacggg taattttacc ctgcactggc gtggatggcg
    24001 acgtataaac ccgataaccg gcaccgcgca gcttaccgac aatctcattc actttatcgg
    24061 catttttcag cgcgcccagt tgcacaacat aggctttacc cgttggcgct gctttttctt
    24121 ccacgaccgg cttcgacgca ggtggtgctt caaccttcgg tttaggcgac tccaccggtt
    24181 tcggcttcgg tggggcgacc ggtgcaggtt ccggttcaaa ctcggtgtta ttgactgcaa
    24241 tagtggccgg atcgagcgac ggtgctgcgg catcacctgc ccgcacctct tccgctgcgc
    24301 cttccggcgg ctgcgtcggc agcgcctggg tggcggctgg catcatatca ggctcatcgc
    24361 gatcgcccgc tttcggcacc agcgggatag ccgcgaactc atcctgataa tgttttttct
    24421 gcccgtccag cagccctgga agtacaatca cccccagcgc caccagcacg atcgtgccca
    24481 ctaaccgatt ctgaaactta cttgccaccg cttctcctcg cgtcaatcac ttccatgaca
    24541 tgtgcgaccg tgtggaacga accacacacc agcacggtat cttccgcttt agcgtccgcc
    24601 attgcggcat cccatgcctg cgccacgcta tcaaatgatt tgccgttacc caaatgctca
    24661 agcagttgtt ctgccgtggc accgcgcggc ccttccagtg gcgcacaata ccagtcatca
    24721 accacgcttt tcaaccaggc cagagttccg gcaatatctt tatcatgtag cataccgata
    24781 accgccagca cgcgcccgtt tttcggtagc gctttcatac gcccggtgag atattccgcc
    24841 gcatgtggat tatgcgcgac atcaaaaata acgcgtggcg actcgctcac aatctggaaa
    24901 cgtcccggca aaattgcgct ggcaatcccg tcgcgaatgg cattttcact gacctccagc
    24961 ccgctggcac gcagtgccgc cagcgccgtt gcggcattcg gttgcgggac aagcggcaac
    25021 ggcagatttt ccagcgtgcc gtgagcatcg ctaaacgccc agtcatgatc ggtgacggaa
    25081 tagttccact caacgccccg acgttgtaac agtgcacttt tttcctgcgc cacatcagca
    25141 atggtagaag gcatttccgg ctcaccgaca attgccggtt tttcgctgcg gaagatgcct
    25201 gctttctcgc gaccaatgct ttcgcgatct ggccccagcc agtcggtatg atccagcgca
    25261 atgctggtta ctaccgcaac atcggcatcg acaatattgg ttgcgtccag acgaccgccc
    25321 agccctactt ccagaatcac cacgtcaagt tgtgcctgtt tgaacagcca caacgccgac
    25381 agcgtaccgt actcgaaata ggtcagggaa atatcaccgc gtgccgattc aatctccgca
    25441 aaagaggcgg tgtgggccga ttccggcaat tcctggccct gcacacgtac gcgctcggta
    25501 taacgcacca gatgcggcga agtgtagacg cccactttgt accctgccgc catcagaatc
    25561 gactccagcg tacggcaagt ggtgcctttg ccattcgtac ccgcaacggt aaacacaaat
    25621 ggcgcaggtt tcaggacgcc aagacgcgcc gcgacctggc tcacgcgctc aaggccgaga
    25681 tcgatagttt tactgtgcag gttttccaga taagaaagcc acgaagccag aggcgacgcg
    25741 gcttgaggag tgcgtttgat aatcatggta tccgctgatt cgttacggtg agaatagcaa
    25801 aagggcagag ccagtggccc tgcccttatc agttatcagg cctcaggttc ctgatctggt
    25861 accggtggca ccactacgcc ttcacgcggc gcttcaggat tcggcgctgg cagattcatc
    25921 aacttcgcca gaatgctcgc cagtttcagg cgcatttccg gacgacggac gatcatgtcg
    25981 atcgcgcctt tctcgatcag gaattcactg cgctggaatc ctggcggcag tttttcgcga
    26041 acggtctgtt cgataacacg cggaccggca aagccgatta acgctttcgg ttcagcgatg
    26101 ttgagatcgc ccagcatggc gaaacttgca gaaacaccgc ccatggtcgg gtcggtcagc
    26161 acggagatgt acggcaagcc gcgctcctgc atttttgcca gcgccgcaga ggttttcgcc
    26221 atctgcatca gcgacatcag tgcttcctgc atacgtgcgc caccagaggc ggagaagcag
    26281 atcagtgggc agttatcttc cagcgcctgc tcaacggcac gcacgaaacg tgcacctacc
    26341 acagacccca tggaaccgcc cataaaggcg aactcgaatg ccgcagcgac aactggcatt
    26401 ccatacaggg tgcctttcat caccaccagc gcatcttttt cgccggtttc tttctgcgca
    26461 gatgccagac ggtctttata cttcttggag tcacgaaact tcagcacatc tttcggctca
    26521 agctcgctac ccagctccac aaggcttcct tcatctaaca ggctatgcag gcgattacgc
    26581 gctgtcatac gcatgtgatg gtcacacttc ggacagacct caagattacg ttccagctca
    26641 gcgcggtata aaacctgacc gcagctatca cacttagtcc acaccccttc aggaatgctc
    26701 gccttgcggg tgggagtaat gttgctttta attcgttcaa tccagctcat tagggacctt
    26761 tctgtctgaa cctggttcga tgccagtttt atctttgggg acgcataatg ccatttttgc
    26821 ccccaacaga ccatgaatgt tgcacattaa aacataacag cccgaaactt tggataaaaa
    26881 agtggtcgaa ccgcggagtt actttttatt ttgcggcgcg tgccgcagcg cgtttgtggc
    26941 gaataaattc gaaaacacct ggcaatattg aaacaaaaat aatgccaaca atcatcagtt
    27001 taaggttgct ctgaatgaag gggagcgttc cgaagaaata acccgcgtag gtaaaaatca
    27061 gtacccatag caacgcgccg atgacgttaa aagcggcgaa atgacgatac gacatgtggc
    27121 ccattcccgc aacgaacggc gcgaacgttc tgacgatcgg cacaaaacgg gcgagaataa
    27181 tcgttttgcc gccatgtttc tcataaaact gatgagtctt atcaagataa ctgcgacgaa
    27241 agattttcga atccggatta ctgaacagtt tgtcaccaaa taaccgtcca atggtgtagt
    27301 tcaccgcgtc acccacaatc gcggcgatca gcatcagcac caccatcata tggacgttga
    27361 gatcgttggt ttccagtgat gccaacgcac cggcaacaaa cagcaatgaa tcacccggta
    27421 aaaacggcgt taccaccaga ccggtttcac agaacaaaat taagaacaaa atggcataaa
    27481 cccagacgcc atactccgcg accagttctg ccagatgcac gtcaatgtgc aggataaaat
    27541 cgatgaggaa ataaatcagg tccatattag cctatgcctt gtactcgtca tctttcaggc
    27601 tgtaactacg ttggctacag cctgaaatgt tccgagcatt attcttgtta gtccgccaga
    27661 aatagcgggc ccattggcgg ttttggaaga tcataccggt cagggtaatc caccgcgacc
    27721 agatacagcc cttccgcttt tgccgttgct gccgccagcg ttctgtcctt tgccgccagc
    27781 aactctgcta tccagctctc cggctggttg tgggcaccga cttccatcag gctgccgaca
    27841 atattcctga ccatatgatg tacaaaggca ttcgctttga tatctaccac cacataagga
    27901 ccgtgacgcg tgacattaat atgcataacg ttgcgccacg gggttcggga ctggcactgc
    27961 accgcacgga acgaggtgaa atcattctcg cccagcaagc attgcgcagc ccgatgcatc
    28021 cgttcagcgt ccagcggttc gtaaaaatgg gttacccctt tactcagtac cgccgggcgc
    28081 agccgatgat tgtagatgat gtagcgataa cggcgagccg tggcgctaaa tcgggcatga
    28141 aaatcatcag gtacagcttt aacccaacgc acggcgatgt caccaggtaa attcgcattt
    28201 acgcccaacg tccacgcagc gtctttgcgc tgtgcggtcg tttcgaaatg cacaacctgc
    28261 ccggtaccgt gtacaccggc gtcagtacgc ccggcgcaga agacggtgat gggttcgttc
    28321 gccacctggg agagcgcctt ttccagcttc tcctgcacac tgcggacttc attctgccgt
    28381 tgccagccgt aatacctact gccgtcgtac tcaatgccca gcgcaatttt ataaactggc
    28441 ggttgttgct ggtcggacat tagtacagat actcctgcac cagtttctcg gcgattttta
    28501 ctgccatcag cgcgccgcca aagcgaacgt tatcggccac cgaccagaac tgaacttgct
    28561 ccggcatacc gtagtcatta cgtacgcagc caacagaaag atgcggcgta ccagaagcat
    28621 ctcctacctg agtcgggaat tcgttctctt cagagagcac aatatcttcg ccctgagcaa
    28681 acgcatcacg cgcttcttct gccgccagcg gacgcagtgc ttcaaagttg accatctggg
    28741 catgaccgta gaataccggt gcctggacga cgcttgccga aatcatcagc ccttcgtcct
    28801 gcaggatttt gcgtacttcg tcaacgatac gacgttcttc acgcacgcta ccttcgctat
    28861 ccggcagtaa cggcagcatg ttgaacgcca gctgacgccc gaagaaatct tcttcgtcaa
    28921 tcggaatgcc attgagtaat ttagcactct gccccgctaa cgcatcgacc gcttttttgc
    28981 cctgggcgga ggctgaaatc aggctggtaa cgctgatacg tgataaaccg ccctgatcaa
    29041 ttagcggttt cagtgccgcc agcagctggc tggtcagact gtccggtacg gcgatgacat
    29101 tccggttgcg gtaatcggtc agtacaaacg ggtttacttc cggcactacc agcggtacgt
    29161 caggttcgag agcaaacaat ccgctgctgt cgatcaccag acaacctgag ttggtcgctt
    29221 cttcaaccca ggcagcggta gcttctttgc ctgcgacaaa aaatgccagc tgcgcctgcg
    29281 tccagtcaaa ttcagcggca tcctgcacgg tgattgtctt accaccaaag cgcagttgtt
    29341 cgcctgcgct ttcgttacgt gccagtgcat aaatttcccc aaccgggaac tgacgttcag
    29401 ccagcgtttc aagcagggct tcgcccacag cgccagttgc gcccaggacg gcaatgttcc
    29461 agccttcaga catggtggtt tactccagaa atagcaaagc tccctgccaa tgttacagca
    29521 gagagcatga agaagagatt aacgtgccgg atgatgaacg gcgttaaaac ccagtttaca
    29581 cagcaatgat gccgcactgg cgtcatcaca aattacatac agagatgacc attcacggcg
    29641 ctcaagatag tttttgcgca gtttatcgaa ctcacccggt atcccggcga ctttacgcag
    29701 tggcgcatca tcgcggcgca catcatacac caaatgcacc agccttttca gcgtcggttg
    29761 atcgagcgga ccatgcagcg taatgcgacc aaactctggc gcgggcagta atgtatccag
    29821 cgcaacgtgt tgttcatgcc caatgaattt gctataggct tcaaacactt gcgtagtacc
    29881 gcgtgcttta ccttccaggg tatagcctgc aatatgcggc gtgccgatat ccacttttgt
    29941 cagcaattcc acattaagtt ccggctcgcc ttcccagaca tccagcacca cgcttaactt
    30001 ctggccttca ttcaggcagg tcagcagcgc ggtattatcg acgactgcgc cacggcaggc
    30061 gttaatcaga atcgctccgg gcttcaggct acggatcagt ttttcatccg ccagatgtcg
    30121 cgttttgtac ggaccatctt taaagagtgg cgtatggaaa gtcagaatat ccgcatgctg
    30181 gactaattca tccagcgagc ggaaatcgcc ctcatcccca cggtcggcgc gaggcggatc
    30241 gcaaagcaag gttgtaatcc ctaacgcttc cagtcgtgcc tgtaaacgcc gaccaacgtt
    30301 accaacgccc acgatcccca cggtacgctc gtgcagtgaa aatccatcgc gttcagcaag
    30361 catcagcagt gaggagaaaa catattccac tactgcaatc gcattacagc caggtgcagc
    30421 ggaaaaacca attcccgcct gctttaacca tgtgtcatca acatggtctg tccccgcggt
    30481 ggcagtgcca acaaatttaa tgggttttcc tgccagcaaa gattcattca ctttcgtgac
    30541 cgaacgcacc atcagcgcat ccgcgtctgc cagttgagcc acggggatgg gacgcccggg
    30601 aaccgcggtc acctcaccca aacggctaaa taagtcgcgg gcataaggca tattttcatc
    30661 aacaaggatc gttaataatt tttcagtacg cgacaatctg gccccccctt tatcagcaaa
    30721 caccctttta accccaccgt acaacatgaa aaccatttca taatcataca catacaaacg
    30781 caaccattta gacgggtgat actggaaagt atgtttcaca gtatcaccct taaagctatt
    30841 tcagaccaga caaaatcact ctcccgtgga caccgagtgg acacttacgc cacctttcag
    30901 cggattaaga gccaccgcat cctgcaaata atccggcgca aaatgtgcat acgccattgt
    30961 ttgctggata gtcgcgtgac ccaggatcct ctgtagcgca ataatgtttc ccccgttcat
    31021 cacaaaatga ctggcgaacg tatgtcgcaa cacatgcgcc gcctgtcctt ttggcaaatc
    31081 gggtttaacc cttttcagca ccaggcaaaa ttcccggtac ttcacatcaa ataatccccc
    31141 cgtctcgctg gttttgatcg cctcacaaac cgcctgcgag attggcaccg ttctcttacg
    31201 accgtttttg gtctcaagaa acgtcacacg attatgaact atctgttcac cacgaagctt
    31261 actggcttcg ccccagcgtg cccccgtact taaacacaaa agcgcaacac gccagtaatc
    31321 gccctctagt gtatcgagca ataacgccac ttctttctgg gacaagaaag ccatttctcg
    31381 cggggacaca taaagcacag agattcccct gaccgggtgc tctgcatccc atagtcctat
    31441 ttttttcaga gcggtaaaca ttccggataa ccggttcata tacctgttag ccgatgacgg
    31501 cttcagtccg tcagcgatcc tgcgagaacg ccaggcaatg attttcagct tatccagatc
    31561 caccgcctgc atatcagcac caagttcatt gattatgttg cgtaattgtt ttcggtcttc
    31621 ctccgcttta cgcctgtgct ggccgtgata cacccaccac aactccagca aatcgttcaa
    31681 cgtccggcga tcacggtagc cctgtatata ttcccgcttc tcagcattcg ccatgatgta
    31741 gcgctcagtg gccaccgcta ccgatttttt gttaaatacc ttacgcacgc gttttccctt
    31801 gcgtccgttc ggcctgatat ccagcaaata acgaccatct tcgagcttct taatcgacat
    31861 ggcgaagccc tccaatgaac cgttttacaa tttcaccagc ttcttcccag caatagtcag
    31921 accaggcaaa aagcaggtcc aaccagtttt ctggcctcag cggggtaatt ttcgggttgt
    31981 ttcggttgag tcctgattgc cctccaaatt gcaggagcca tcaagagaga gagccggggc
    32041 tatctgtccg gcctccggca ttgtttcatc agttgataac cagtaggcgt acttcttaaa
    32101 tttagggtgt tttgtaacct taagtaaggc cccctctgtt acttgcttcc ccctcacctc
    32161 atagttagtt actgtcccat aaggcaaccc cacgcaatcc gcaaactcct ggcgggtcat
    32221 accttcagct tcacgtatca ggcgaaattt ttcacccatg cttgacaaag atgccatatc
    32281 gggcatatcc tccattgcaa acgtgccaca tcgggcacac acataagaag ccaaaataag
    32341 ccactttggg ccattttgag ccattcgaac gaatcaagga gattaccaca atgagagaat
    32401 cagagcttga gggcttcatt caggtagcgc catatccact tgaggcggtg ccatatcaac
    32461 tattcgccaa gatgattggc cgcaaagaat ccaccgtcag aaccatgatt gacgccgcaa
    32521 agctaccaac aattgacttt gtgaaaccgg gttcagtgaa aactcgtgca tcagaaaact
    32581 gggtatatct gccagcattt aacgcaggta tgcgaaaagc attttttaat caacccaaag
    32641 aacaacgcga tgcatggctg ttatggctgg gtctgtaacg atatatccag gaatttttca
    32701 aaaccaatat atctactctg agagagttgt ttgggcataa aaaagcacag gataactaag
    32761 ctgataaccc actacagaca caaaaggaaa cagccatgac tacacaaaaa gcaaaaacca
    32821 taattaaacg atacaacgta cgtagtggtg tggctggcgt tgtcttttcc gatgtgacac
    32881 agcgacgcag cttccgcact ggcacagaac gccacgcagc ccgtttctca accagtgcat
    32941 cacgcagcaa cacgcactac agcctgcagg aaacgcacgc cacgcctgac gggcatctgg
    33001 ttaaacaaac cggtaaacac acctggctga ttaatgatgc cggaattgtc atacacagag
    33061 gcgcacgcaa cccgattacc ggaaatacct gttactccct gactcgcgga gatgagcagt
    33121 tcgggcagga tttcaccctg cacgaagcat taagaacggc agaccggtta atcagcggtc
    33181 gcagttttca gcacccg
//
LOCUS       DAZGHF010000044        32809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGHF010000044 DAZGHF010000000
VERSION     DAZGHF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.044"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            26..226
                     /locus_tag="ACHK3A_002155"
                     /pseudo
     CDS             26..226
                     /locus_tag="ACHK3A_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
     gene            749..850
                     /gene="ykgR"
                     /locus_tag="ACHK3A_002156"
     CDS             749..850
                     /gene="ykgR"
                     /locus_tag="ACHK3A_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO8740235.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            1215..1478
                     /gene="ykgM"
                     /locus_tag="ACHK3A_002157"
     CDS             1215..1478
                     /gene="ykgM"
                     /locus_tag="ACHK3A_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO8740236.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            1478..1618
                     /gene="ykgO"
                     /locus_tag="ACHK3A_002158"
     CDS             1478..1618
                     /gene="ykgO"
                     /locus_tag="ACHK3A_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO8740237.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1653..1878)
                     /gene="ykgL"
                     /locus_tag="ACHK3A_002159"
                     /pseudo
     CDS             complement(1653..1878)
                     /gene="ykgL"
                     /locus_tag="ACHK3A_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            2702..3244
                     /gene="ecpR"
                     /locus_tag="ACHK3A_002160"
                     /gene_synonym="matA"
     CDS             2702..3244
                     /gene="ecpR"
                     /locus_tag="ACHK3A_002160"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO8740238.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            3318..3905
                     /gene="ecpA"
                     /locus_tag="ACHK3A_002161"
                     /gene_synonym="matB"
     CDS             3318..3905
                     /gene="ecpA"
                     /locus_tag="ACHK3A_002161"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO8740239.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            3962..4630
                     /gene="ecpB"
                     /locus_tag="ACHK3A_002162"
                     /gene_synonym="yagY"
     CDS             3962..4630
                     /gene="ecpB"
                     /locus_tag="ACHK3A_002162"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO8740240.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            4656..7181
                     /gene="ecpC"
                     /locus_tag="ACHK3A_002163"
                     /gene_synonym="yagX"
     CDS             4656..7181
                     /gene="ecpC"
                     /locus_tag="ACHK3A_002163"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO8740241.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            7171..8814
                     /gene="ecpD"
                     /locus_tag="ACHK3A_002164"
     CDS             7171..8814
                     /gene="ecpD"
                     /locus_tag="ACHK3A_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO8740242.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            8783..9493
                     /gene="ecpE"
                     /locus_tag="ACHK3A_002165"
     CDS             8783..9493
                     /gene="ecpE"
                     /locus_tag="ACHK3A_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO8740243.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQTGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            9806..10135
                     /gene="ykgJ"
                     /locus_tag="ACHK3A_002166"
     CDS             9806..10135
                     /gene="ykgJ"
                     /locus_tag="ACHK3A_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO8740244.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(10147..10313)
                     /locus_tag="ACHK3A_002167"
                     /pseudo
     CDS             complement(10147..10313)
                     /locus_tag="ACHK3A_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(10384..10998)
                     /gene="yagU"
                     /locus_tag="ACHK3A_002168"
     CDS             complement(10384..10998)
                     /gene="yagU"
                     /locus_tag="ACHK3A_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO8740245.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(11213..11398)
                     /locus_tag="ACHK3A_002169"
     CDS             complement(11213..11398)
                     /locus_tag="ACHK3A_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740246.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            11447..11604
                     /locus_tag="ACHK3A_002170"
                     /pseudo
     CDS             11447..11604
                     /locus_tag="ACHK3A_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835868.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(11619..11694)
                     /locus_tag="ACHK3A_002171"
     tRNA            complement(11619..11694)
                     /locus_tag="ACHK3A_002171"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11659..11661),aa:Thr,seq:cgt)
     gene            complement(11809..13062)
                     /gene="proA"
                     /locus_tag="ACHK3A_002172"
     CDS             complement(11809..13062)
                     /gene="proA"
                     /locus_tag="ACHK3A_002172"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO8740247.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAGALAQLQTGPAKVVAVKTEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     SRGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(13074..14177)
                     /gene="proB"
                     /locus_tag="ACHK3A_002173"
     CDS             complement(13074..14177)
                     /gene="proB"
                     /locus_tag="ACHK3A_002173"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO8740248.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            14466..15521
                     /gene="phoE"
                     /locus_tag="ACHK3A_002174"
     CDS             14466..15521
                     /gene="phoE"
                     /locus_tag="ACHK3A_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO8740249.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLESDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(15560..15961)
                     /gene="crl"
                     /locus_tag="ACHK3A_002175"
     CDS             complement(15560..15961)
                     /gene="crl"
                     /locus_tag="ACHK3A_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO8740250.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(16019..17263)
                     /gene="frsA"
                     /locus_tag="ACHK3A_002176"
     CDS             complement(16019..17263)
                     /gene="frsA"
                     /locus_tag="ACHK3A_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO8740251.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(17355..17813)
                     /gene="gpt"
                     /locus_tag="ACHK3A_002177"
     CDS             complement(17355..17813)
                     /gene="gpt"
                     /locus_tag="ACHK3A_002177"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO8740252.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            18074..19531
                     /gene="pepD"
                     /locus_tag="ACHK3A_002178"
     CDS             18074..19531
                     /gene="pepD"
                     /locus_tag="ACHK3A_002178"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO8740253.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(19588..19940)
                     /gene="prfH"
                     /locus_tag="ACHK3A_002179"
                     /pseudo
     CDS             complement(19588..19940)
                     /gene="prfH"
                     /locus_tag="ACHK3A_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(19936..20142)
                     /locus_tag="ACHK3A_002180"
                     /pseudo
     CDS             complement(19936..20142)
                     /locus_tag="ACHK3A_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(20460..20912)
                     /gene="yafP"
                     /locus_tag="ACHK3A_002181"
     CDS             complement(20460..20912)
                     /gene="yafP"
                     /locus_tag="ACHK3A_002181"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8740254.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFSMRYKPQH"
     gene            complement(20909..21964)
                     /gene="dinB"
                     /locus_tag="ACHK3A_002182"
     CDS             complement(20909..21964)
                     /gene="dinB"
                     /locus_tag="ACHK3A_002182"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO8740255.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(22035..22820)
                     /gene="lafU"
                     /locus_tag="ACHK3A_002183"
     CDS             complement(22035..22820)
                     /gene="lafU"
                     /locus_tag="ACHK3A_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO8740256.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            22792..24504
                     /locus_tag="ACHK3A_002184"
                     /pseudo
     CDS             22792..24504
                     /locus_tag="ACHK3A_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(24722..25219)
                     /gene="rayT"
                     /locus_tag="ACHK3A_002185"
     CDS             complement(24722..25219)
                     /gene="rayT"
                     /locus_tag="ACHK3A_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO8740257.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKQ
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLQNMWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
     gene            complement(25396..26145)
                     /gene="yafL"
                     /locus_tag="ACHK3A_002186"
     CDS             complement(25396..26145)
                     /gene="yafL"
                     /locus_tag="ACHK3A_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8740258.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            26457..27197
                     /gene="dpaA"
                     /locus_tag="ACHK3A_002187"
     CDS             26457..27197
                     /gene="dpaA"
                     /locus_tag="ACHK3A_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO8740259.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(27168..27935)
                     /gene="yafJ"
                     /locus_tag="ACHK3A_002188"
     CDS             complement(27168..27935)
                     /gene="yafJ"
                     /locus_tag="ACHK3A_002188"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO8740260.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(28051..28629)
                     /gene="lpcA"
                     /locus_tag="ACHK3A_002189"
     CDS             complement(28051..28629)
                     /gene="lpcA"
                     /locus_tag="ACHK3A_002189"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO8740261.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            28869..31313
                     /gene="fadE"
                     /locus_tag="ACHK3A_002190"
     CDS             28869..31313
                     /gene="fadE"
                     /locus_tag="ACHK3A_002190"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO8740262.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(31356..31802)
                     /gene="ivy"
                     /locus_tag="ACHK3A_002191"
     CDS             complement(31356..31802)
                     /gene="ivy"
                     /locus_tag="ACHK3A_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO8740263.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            31983..32753
                     /gene="yafV"
                     /locus_tag="ACHK3A_002192"
     CDS             31983..32753
                     /gene="yafV"
                     /locus_tag="ACHK3A_002192"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO8740264.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWRDADEFRLW"
BASE COUNT         8301 a         8346 c         8208 g         7954 t
ORIGIN      
        1 tcagataaac atttgaggta ttgtcatgac aaataaacac ctatcactgt tttctccttt
       61 tatgctgaca gaaaaaataa agccatgtaa tcgtatcgtt atggctccta tgacgacctg
      121 gtcagctaat cctgatggaa ccatctctga gcaagaactc gaatattata aacgtcgctc
      181 acaaaatatt ggtttagtaa ttacaggata tacctatgtc acgccaagga aattcagaca
      241 tcgaaaggaa ctggctggtt tccgttaatg gattaaatat cgattactct cccgagagta
      301 aaactatcaa atcaatgagt aaccgataca gcctcgcaaa ttctttcaga taattaaccc
      361 gtcactgcgg cacgttctct cgtaaggcgt gcctgcagtg taatccagta tgtatccggc
      421 atagttgacg gtttaataga ctcaattcct ctccctggtt acctacaacg atatcttgta
      481 taacacccca ggagatacga cgttacgatg agatgcaata tgctcctcag cgccaatcaa
      541 cgccaggact gggccagtct tgagatcaga ctcattcccg taactgattt caggcgacat
      601 atatcaatga cgttggggca ataaaagata gccgatgtaa tacatctatt caaatttgtg
      661 atacatgtca aaaaattggt tgaccaaact cgttatttat ataagggcac ttacgaagtg
      721 cactcttttt taaagcgagg aagtaccaat gaaagagaat aaagtacagc aaatcagtca
      781 taaactgatt aatatcgttg tttttgtcgc aattgtagaa tacgcctatt tatttctcca
      841 tttctattaa taacggcaat aaactgttca cttcagtgat atttaaaata tgcatcctct
      901 cccttttttg taagtaatta ttatatccgt gggagaggaa tacacattgt caggtaatca
      961 atcatgctgc aataaatcat cggccagtaa agtggagata gcctccattc tcgaaaaatc
     1021 catactctca gcgaaaccat catcaatcac tcatccaggc gtttatggga gcgtcgccaa
     1081 tggctgctaa caatgccaga cttccccgtt gcggaaattc cacatcccac aaatagtcac
     1141 agtgattggg tgttgaaatg atccggatga gcatgtatct ttacggttat gttataacat
     1201 aacaggtaaa aatgatgaaa cccaatatcc atcctgagta tcgtactgtg gtgttccacg
     1261 acaccagcgt tgatgagtac tttaaaatcg gctcgactat caaaacagac cgtgagattg
     1321 agctggatgg cgtaacgtat ccgtacgtaa caattgatgt ctcttcgaaa tcgcacccgt
     1381 tctatacagg gaagctgaga acagtggcat cagaaggaaa tgttgcacgg ttcacccaac
     1441 gttttggtcg ttttgttagc acgaaaaagg gggcatgatg aaagtcctta actctctgcg
     1501 taccgcaaaa gaacgccatc cagactgtca gattgtgaag cgaaaaggac ggctgtatgt
     1561 gatttgtaaa tctaatccac gttttaaggc cgttcagggt cgtaagaaaa aacgttgatt
     1621 caaaattcga cgaattaacg atatttgtct gattaataat cataccggat taatgttggt
     1681 gtatttatta caccagcatt aattttcctg gggatgtatc ttcctgttaa tttaaggtca
     1741 actgcctgac gtttctctcc gaatattcca ttatattaat gttgacttgt tgaccagctg
     1801 cgccctgtat gctggcatca actctatttt agactgaaca cgccactcag tttcctccct
     1861 ttgcgacgca gcctgcattt acactcaaac tgttaagatg ataaatgtgg taaatctgtt
     1921 ggtactgaca taaaaacgtt tacgccacag gaacggccag atccatcggt aaccccatcg
     1981 ccgacgttcg agtgccagtt agagtaacgc gtacagatga ctgaatgcag tgccctggca
     2041 aagaggccat cgttcctgtg acaactggca gtcttcgttt aacttcactt aatttggctc
     2101 ttggggggct taccggacag atgacgtact tacacctgtt taatttttca tcacttattg
     2161 ggatgaacac ccacaaccat tttgtgcggc atgttaatcc attaaaacac cttactgatt
     2221 ggcaaatcat ctttaattat gacttatgat agttttatat tccatttctt atcatttaaa
     2281 cttgtttttt tactagtcca ttacacaaca cattaagact attcctaaca cctcagggca
     2341 aagttcctgg ctaatataaa atgcaagtaa gaattgaacg ttatattgcc aataacctta
     2401 tgaaactgaa tgtctttttc ttcttatcaa aaaagcaata ttttcatttt ttgtaaatat
     2461 tgacttaacc atggaattca ttttctgttc acatattgac actcatcagg aaaaaaacat
     2521 aaatttaaac ctaatcgaaa taattaaaac ttaatctcgt ttaacctata ttgatatgtg
     2581 ctacgtatct tatttacttc cgatttacta aagaaactga atgtacctgt aaaaattaca
     2641 ggtttggaaa gtagtgacat ggcaaaatga ttacagcagg gactatgagg ttaaaaacca
     2701 tatggaatgt caaaaccgtt ctgataaata catctggtct ccccatgacg cctacttcta
     2761 taaaggacta tctgaactga ttgtggatat cgacagatta atttatctat cgctggagaa
     2821 aatcagaaaa gatttcgtgt ttatcaatct caatacggat tctttaactg agtttataaa
     2881 ccgtgataat gagtggttat ccgcggtaaa ggggaaacag gtcgtattga ttgcggccag
     2941 aaagtcagaa gccttagcaa attattggta ttacaacagc aatattaggg gcgtggtata
     3001 cgctggactg agtcgtgata ttagaaaaga actggcctat gtgattaatg gcaggttcct
     3061 gagaaaagat attaagaaag ataaaatcac tgaccgggaa atggaaatta tccgcatgac
     3121 ggctcaggga atgctgccta aatcgattgc cagaattgaa aattgtagtg tgaagacagt
     3181 gtatacccat cggcggaatg cagaggccaa gctgtactca aaattatata agttggttca
     3241 gtaaactcca agcaagttag ttttaaaaat gactcaccgg gacttcatgt cctcaattca
     3301 actcgggaag aaaagcaatg aaaaaaaagg ttctggcaat agctctggta acggtgttta
     3361 ccggtacagg tgtagcgcag gctgctgacg taacagctca ggctgtagcg acctggtcag
     3421 caacagccaa aaaagacacc accagtaagc tggttgtgac gccactcggt agcctggcgt
     3481 tccagtatgc cgaaggcatt aaaggtttta actcacaaaa aggtctattt gacgtggcta
     3541 tcgagggtga ctcaacggct accgccttta aactgacctc acgccttatc accaacacct
     3601 taacccagtt ggatacctca ggttccacac tgaatgtggg cgtggattat aacggcgcgg
     3661 cagtcgaaaa aactggcgat accgtgatga tcgataccgc caacggcgta ctgggcggca
     3721 accttagccc actggctaac ggttacaatg ccagcaatcg taccaccgca caggatggtt
     3781 tcactttctc catcatcagc ggcaccacca atggtaccac cgcagtaacc gattacagca
     3841 ctctaccgga aggcatctgg agcggcgacg ttagcgtaca gttcgacgct acctggacca
     3901 gttaatctct ctgatgtacc agcaggggta cccccctgct ttattcctgg atggaattgt
     3961 tatgaaaaag caccttctgc ctctcgctct gctgttttcc ggaatatcac cggcccaggc
     4021 gctggatgtc ggcgatatat catcgtttat gaacagtgac agcagcacgc taagcaaaac
     4081 gatccaaaac agtaccgaca gtggccgcct catcaatatc cgtctcgaac ggctctcttc
     4141 accgcttgac gacgggcagg ttatcgcaat ggacaagccg gatgagttgc tactcactcc
     4201 cgccagcttg ctgctacccg cccaagccag cgaagtgatc cgcttcttct ataagggacc
     4261 agcagatgaa aaagagcgct actaccgcat tgtctggttt gatcaggccc tcagtgatgc
     4321 gcagcgcgat aatgccaacc gcagcgctgt ggccactgct tccgcccgca tcggcaccat
     4381 tctggtcgtc gcaccccgcc aggcaaacta ccactttcag tacgccaacg gctccctgac
     4441 aaatacagga aatgcgacgc tgcggatcct cgcctacggc ccctgcctga aagccgccaa
     4501 tggtaaggag tgtaaagaga attactacct gatgccgggc aagtcgcgtc gttttacccg
     4561 cgtggacacg gcggataaca aaggacgggt tgcactttgg cagggtgata agttcattcc
     4621 cgtgaaatag atagctgtgc agatggataa cgacaatgcc tttacgacgg ttctccccag
     4681 gactgaaagc ccagtttgcc ttcggcatgg tctttttgtt cgttcagccc gatgccagcg
     4741 ctgctgacat aagtgcgcag caaataggtg gggtgattat tccgcaggcc ttcagtcagg
     4801 cgctccagga cggcatgagc gtcccgcttt atattcatct cgccggtagc cagggtcgcc
     4861 aggacgatca gcgaatcggc agcgctttta tctggttgga tgatggacag ttacgcatcc
     4921 gaaaaataca gctggaagag agtgaagata acgccagtgt cagcgaacaa actcgacagc
     4981 agctgatgac tctggccaac gccccgttca atgaggccct taccatcccc ctgactgaca
     5041 acgcgcagtt ggatctcagc ttgcgccaac tgctgctgca gctggtggtc aagcgcgaag
     5101 cgctgggcac cgtactacgc tcacgtagcg aagacatcgg gcagtccagt gttaacaccc
     5161 tcagcagtaa tctgagctat aacttcggcg tctataacaa ccagttgcgt aacggcggga
     5221 gcaacacctc cagctatctg tcgctgaata acgttactgc gctgcgcgaa catcacgtgg
     5281 tgctcgacgg ctcactgtac gggattggta gcggtcaaca ggacagtgaa ttatataaag
     5341 cgatgtatga acgcgatttt gctggtcacc gatttgccgg tggaatgctc gacacctgga
     5401 acttgcagtc cttagggccg atgaccgcca tttcagcagg gaagatttac ggcctttcct
     5461 ggggaaacca ggccagctcc accatcttcg acagcagcca gtcggccacg ccagtgatcg
     5521 cctttttacc ggcggcgggc gaagtacatc tcacccgtga tgggcggcta ctaagcgttc
     5581 agaacttcac catgggcaat catgaagtgg atacccgggg tctaccgtac ggtatttacg
     5641 atgtggaagt tgaggtaatc gttaacggtc gcgtgatcag caaacgcacc cagcgggtca
     5701 ataagctgtt tagccgggga cgtggcgtcg gtgcaccgct ggcgtggcag atatggggcg
     5761 gtagctttca tatggatcgc tggtcggaaa acgggaaaaa gacgcgacca gccaaagaga
     5821 gttggctggc aggtgcctcg acctccggct cactgagtac gtttagctgg gcggcaacgg
     5881 gatatggata cgataatcag gcggtgggtg aaacccgtct gacgctgccg cttggggggg
     5941 cgatcaacgt taacctgcaa aacatgctgg ccagtgacag ctcatggagc aacatcgcca
     6001 gcatcagcgc cactctacct ggaggcttca gttcgctgtg ggttaaccag gaaaaaaccc
     6061 gcattggcaa tcaattgcga cgtagcgatg ccgacaaccg tgcaatcggc ggcacactca
     6121 acctgaactc actgtggtcg aagctgggta cgttcagcat cagctacaat gatgaccgcc
     6181 gctacaacag ccattattac acggcagatt actatcaaag tgtctacagc ggtacctttg
     6241 gttcgcttgg cctgcgggcc ggtattcagc gctataacaa cggcgacagc agcgccaata
     6301 cagggaaata tatcgctctc gatctctcgc taccactggg caactggttt agcgcaggga
     6361 tgacccatca aaacggctac accatggcaa acctgtcagc acgcaaacag tttgatgaag
     6421 gaaccattcg cactgttggt gccaatctgt cacgagccat ctccggcgat accggtgatg
     6481 acaaaaccct cagcggtggg gcgtatgcac agttcgacgc tcgttacgcc agcggaacgc
     6541 tgaacgtcaa tagcgcggcg gacggctaca tcaatactaa tttgaccgcc aacggcagcg
     6601 tcggctggca gggtaaaaac atcgctgcca gcgggcggac cgatggcaac gctggggtga
     6661 tattcgacac tggcctggag aacgatggtc agatcagcgc caaaatcaac gggcggattt
     6721 tcccgcttaa cggcaagcgt aactatctcc cgctctctcc ctatggaaga tatgaggtgg
     6781 agttgcagaa cagcaaaaac tcactcgaca gttacgatat cgtcagcggt cgcaaaagtc
     6841 atctgactct ctatccaggc aatgtcgctg tcattgagcc agaggtgaag cagatggtta
     6901 ccgtctccgg tcgtatccgt gcggaagacg gcacactgct ggctaacgca cggattaaca
     6961 accatatcgg ccgaacccga accgatgaaa acggcgagtt tgtcatggac gtggataaga
     7021 aataccccac tatcgatttt cgctacagtg gcaataaaac ctgcgaagtg gcactggaac
     7081 tcaaccaggc gcgcggtgcc gtctgggtcg gtgatgtggt ctgcagcggc ctctcatcgt
     7141 gggcggcggt gacgcagaca ggagaagaga atgagagtta acctactgat agcgatgata
     7201 atctttgcgc taatctggcc tgcaaccgcg ctcagagcgg cagtgagcaa aacaacctgg
     7261 gcggatgctc cggcacgcga gtttgtgttt gtcgaaaaca actcagacga caactttttc
     7321 gtcactcctg gcggggcgct ggatccgcgc ctgaccggtg ccaaccgctg gaccggttta
     7381 aaatacaatg gttcaggaac catctatcag caaagcctcg gctacattga taacggttac
     7441 aacaccggcc tttataccaa ctggaagttt gatatgtggc tggaaaattc accagtttca
     7501 tctcctttaa ctggcttgcg ctgcatcaac tggtacgcag ggtgtaatat gaccaccagt
     7561 cttatcctgc cgcaaaccac cgacgccagt ggattttatg gcgcgacggt gaccagcggc
     7621 ggcgcgaaat ggatgcacgg catgttgtca gacgcgtttt accagtatct gcaacaaatg
     7681 cccgtcggca gcagctttac aatgaccatc aatgcctgcc agacctctgt gaactatgac
     7741 gccagcagcg gcgcacgctg taaggatcag gcctccggca actggtatgt tcgcaacgtc
     7801 acccatacga aagcagcaaa tctgcggttg ataaataccc actcgctggc ggaagtattt
     7861 atcaacagcg acggagtacc gactctgggc gaagggaacg ccgactgccg gacgcaaacc
     7921 atcggcagcc gttcaggatt aagttgtaag atggttaact ataccctgca aacaaacgga
     7981 ctcagcaaca cctcaatcca tatattcccg gcgatcgcca actcgtcgtt agcctcggcc
     8041 gtcggggcgt acgatatgca gttcagtctg aatggcagtt catggaaacc ggtgagcaat
     8101 actgcctatt actacacctt caacgagatg aagagcgcag actcgatcta tgttttcttc
     8161 tcgagcaact tctttaaaca gatggtgaac ctcgggatca gcgatatcaa caccaaagat
     8221 ctattcaact ttcgctttca gaacaccaca tcaccggagt ctggctggta tgaattttct
     8281 acctccaaca cgctgattat caaaccccgt gatttcagca tcagtattat ctccgatgaa
     8341 tatactcaga caccgtcgcg ggagggatat gttggcagcg gcgagtcggc actcgatttc
     8401 ggctatatcg taaccaccag cggtaaaaca gctgccgacg aagtgctgat caaggtgacc
     8461 ggacccgcgc aggtgattgg cgggcgctcc tattgtgtct tcagctccga tgacggtaag
     8521 gcgaaagtac cgttcccggc gacgctttcc tttattaccc gcaacggagc tacaaaaacc
     8581 tacgatgccg ggtgcgatga tagctggcgg gatatgaccg atgcgctgtg gttgaccaca
     8641 ccgtggactg atatctctgg cgaagtgggg cagatggata agaccacagt caaattttcg
     8701 attccaatgg ataacgccat ttctctgcgt acggtagatg ataacggctg gtttggcgaa
     8761 gtcagcgctt caggagagat tcatgttcag gcgacgtggc gtaacattaa ctaaggccct
     8821 gctgacagtg gtctgtatgc tggcggcacc tttgacacag gcgatttcgg tcggcaatct
     8881 gactttttca ctgccgtccg agactgactt tgtcagcaaa cgtgtcgtga ataacaacaa
     8941 aagcgcgcgg atataccgta ttgccatcag tgctattgat agcccgggca gcagtgaatt
     9001 gcgcacccga ccggtggatg gtgaactact tttcgccccc cgccagctgg cgttgcagac
     9061 tggtgagagc gagtatttta aattttacta tcatggtcca cgggataacc gcgagcgcta
     9121 ctaccgggtc tcatttcgcg aggtccccac tcgtaacctg acaaaacgca gcccaaccgg
     9181 cggtgaggtc agcacggagc cggtggtggt gatggatacc attctggtag tacgaccacg
     9241 tcaggttcag tttaaatggt ccttcgatca ggtgaccgga acggtaagta acaccggcaa
     9301 cacgtggttt aagctactga ttaaaccagg atgtgattcg accgaagagg aaggcgatgc
     9361 ctggtatcta cgtccgggag acgtggttca tcagcctgag ttacgtcagc cggggaatca
     9421 ttatctggtc tataacgaca aattcattaa gattagcgat tcctgtccgg ctaagccccc
     9481 ttcggcggac taagacttat ccatcggacg agggaagaat tccacctcag agactcaaaa
     9541 atcgtttaaa tgatgtggaa gcgatcgtca atgtcacctg taacgatgag ctttctcata
     9601 tgcacaccac agtgttcagg agacacggaa aggtaccagg cggcctgcta acgagtggtt
     9661 tcttcactaa ttaggacgta aaatcggtaa cggctggaaa tcattcaata ctcacactat
     9721 cgaaagtaca ccagccaacc gcgatacatc cctgcatacg acgtaccgca gttctcttta
     9781 cgatttatag ctgtactggt gaattatgag caatctgaat ccatgcatga cgtgtggtgc
     9841 ctgttgtgca tttttccgcg tctcttttta ctgggccgaa gctgacgatg ctggcggtac
     9901 cattcccgcc aggctcactg aacaaatatc cccttttcac cgatgcatga gcggtactaa
     9961 tcagaaaaat ccccgatgta ttgcccttgc aggaacaccg ggcaaaaatg cctgctgcac
    10021 gatatatgaa aatcgatcgt ccacatgcag agaattcgcc atgtctggtg agaacggaga
    10081 agtcaatgag gcttgcaatc gtgcaagagc taaatacggg ctaacaccgt tataaatata
    10141 caataattaa ttgcattgcc ccgccagctg taatagcggg gcttcgtttt tcagggacaa
    10201 caaaacctga aacttcttcc attttccagc aacaatgcct cttcttctgt agcgttctcc
    10261 ggatctacat catcctgaag tagcgcgata tgatcactga ctgtccgcat cagaatatga
    10321 aaagcaacgt ttttaaccta taaaaatggc gctatatttg cgccattttt atcattcaaa
    10381 gcattatcta tttgagccta aagggatctc aggatctggc tcatgggtaa ttctgtttcg
    10441 taaatctctg cgaataattt caatagacca gaaccagact aaatgtccaa aaatttcaga
    10501 gacattctca taccacggaa gatcaaagag aggtggcgta agtcccatga gagggaatga
    10561 aatcatatga acaaaaagtt gcgctaaagc acctgccagt aagccctgcc aaagtttaat
    10621 tttcggaaat acttcggcga ccacacagta accaacggca aaaactatcg aaaagattat
    10681 gtgcgttaca ccaacccagt taaagacatg cccggcgaag gtataaacag ccgaatttgg
    10741 atctgtcagt cccaaccaat cccgaagaaa aatgtacgga ggattgagaa agttacggga
    10801 gcaatcaatt tggcctgcag ccctgattaa tgattccgga ccacacgctg cattaaacat
    10861 atcaaccgga ctacgtggtg gcaatggaac ttctgccccc cacttaacaa atgcggaaac
    10921 aacgccagca atcagcccaa tgaatgcagc aagaccataa cgtctgcggt ttggtggagt
    10981 ttgttcaaat atattcatat ctaccctgct tgtaccatta tgttatacac ctctttagga
    11041 gtattcataa aacaaggcaa atgtaaagaa ctgtattgtt ttgtataacc acatggttac
    11101 ctaatcgcca attaatataa gctccattat ttctccatat tttcatgtta gaagtgacaa
    11161 ggagaaatgt aatgtttctc tcagagaata taaatcattc cttttgccca ccttaatggt
    11221 gggctttttt atgcaaaacc tgaacgtcct gtggtattcc tctggcataa tgattttcgc
    11281 gattaaccaa caaccagacc agcaaagacg ccaccacaag cacagtaaca tccatcaggc
    11341 ttgccacacc ccatgcgtgc ggctcaaagc cgccatggca tattaatccg cttgccattt
    11401 tcgaacagct gattggtttg gtccggtgtt tccctgcttg cacttaaaaa tgagcggatg
    11461 ggaaactctc gaaatggttc gtcgatatgc tcacctcgca cctaatcacc ttaccgaaca
    11521 cgcacggtaa atagattcga tcctgaaccc atcggtccca aatctgtccc aataaaaaat
    11581 aaggaaggta ttagtgatgt gtaacttatt gatttaaatg gtgccgataa gaggagtcga
    11641 acctccgacc ttcgcattac gaatgcgctg ctctaccaac tgagctatat cggccctgaa
    11701 aggacatgtt cacgaacgtg aatcacggtg gacaaggtta aaactaaccg ggcgatgcgt
    11761 caatggcctt gtgaatcaaa tggctacttt tgcatcaccc ggttttattt acgcacgaat
    11821 ggtgtaatca ccaatgccga tccacttgta agtggtcagt gcttccagcc ccattgggcc
    11881 acgcgagtgg agtttttgtg tgcttaccgc cacttccgca cccagtccaa actggccgcc
    11941 gtcggtaaaa cgcgtagagg cgttaacgta aacagcggag gaatctactt cgttaacaaa
    12001 acgctgggcg ttgcgcatat cgcgggtcag gatcgcatcg gagtgttgcg tgccgtgttc
    12061 acgaatatgg gcgatggcat cgtcaagatc gctgacgatt ttgacgttca aatctaatga
    12121 cagaaactca tcgtcatact cttcggtttt aacagcaacc accttcgcgg ggcctgtctg
    12181 caactgcgcc agtgcacctg catcagcgtg taacgtcacg ccactttccg ccatttgttt
    12241 gcttaatgcg ggcaggaaac tatcggcgat gtttttattc accagcaacg tttcaaccgt
    12301 attacatgtg ctcggtcgct gagttttcgc gttgacgatc actttcaggg cttcagcgat
    12361 ctctgcactt tcatcaacgt aaatatggca tacgcctata ccacctgtga tcaccgggat
    12421 cgtcgactgt tcgcggcaca gtttatgcag gccagcgcca ccgcgcggga tcagcatgtc
    12481 gatgtattta tccatacgca gcatttcact gaccagcgcg cggtcaggat tatcaatcgc
    12541 ctgcacggca cccgccggta agctgcagga tttcagggcg tcctgaatca ccgccaccgt
    12601 tgcagcgtta gtgcgacacg tttctttgcc gccgcgcagg atcaccgcgt tacctgtttt
    12661 caggcacagc gaagcgacat ctaccgtcac gttcgggcgc gcttcataaa tcacgccaat
    12721 cacccccagc ggcacgcgac ggcgctccag acgcagacca ctgtccagta cgccgccatc
    12781 gattacctgc cccaccggat cggcgagatt acacacctga cgcacatcgt cggcgatgcc
    12841 tttcagccgt gcgggcgtca gtgccagacg gtcaagcatc gcttcgctaa ggccattggc
    12901 acgcgcgtca gcaacatcct gggcgttagc gttgaggatg atttcgcttt gtgcttccag
    12961 ttcatcggcg attttttcca gcacacgatt tttttcgcgg ctggagagtt gcgctaattt
    13021 atacgaggct tgctttgcgg caatgcccat ttgttccagc atcggccagc tccttaacgg
    13081 gtaatcatgt catcacggtg aacggcaacc gggccatatt catatcccag tattgcatca
    13141 atttcttgcg agtggtgtcc ggcaatgcgg cgtaatgcat cgctgttgta acgactgacg
    13201 ccgtgggcga tatcgcgacc ttcgaggttg caaatgcgga tgacttcacc acgcgagaaa
    13261 ttgccagtta cgctcttaat gcctttcggc aacagggagc tgccgcgttc aagaatggcg
    13321 gcggttgccc cttcatctac cgtgatttca cccgccggcg gcgcaccgaa aatccagcgt
    13381 ttacggtttt caagcggagt cgcctgggca tggaacagcg taccgacgga aatgccttcc
    13441 atcacatcac caataacgcc cggcttgctg cccgcggcaa taatggtgtc gatacccgca
    13501 cggcaagcca cgtccgcggc ctgcaatttg gtgctcatac cgccagttcc gaggcctgaa
    13561 acgctatcac cggcaatcgc gcgcagtgcg tcatcaatgc cgtacacatc tttaatcagt
    13621 tctgcctgcg gattgctgcg cgggtcagcg gtatacaaac ctttttgatc ggtcagcagc
    13681 aacagtttat cggcacccgc cagaatcgcc gccagcgcag aaaggttatc gttatcaccg
    13741 accttaatct ctgccgtagc gacagcatcg ttctcattaa ttaccggaac gatattgtta
    13801 tcgagcaacg cacgcagggt atcgcgggcg ttcaggaagc gttcacgatc ttccatatca
    13861 gcacgggtca gcaacatctg cccgacatga atgccgtaaa ttgaaaacag ctgttcccac
    13921 agttgaatca gtcgactctg ccctaccgcc gccagcagct gttttgaagc gatagtcgct
    13981 ggcaattccg ggtaccccag gtgctcacgt ccggcggcaa tcgcacccga cgtcacaata
    14041 acaatccgat gcccggcggc atgtaactgc gcgcactggc gaacaagttc aacgatatgg
    14101 gcacggttca ggcggcgcga tccgcctgtt agcacactgg tgccgagttt taccaccagc
    14161 gtctggctgt cactcatgat tctctgccat tcaattttag gaaaaaatga tatcaaacga
    14221 acgttttagc aggactgtcg tcggttgcca accatctgcg agcaaagcat ggcgttttgt
    14281 tgcgcgggat cagcaagcgt agcggcagtt gtttacgctt ttattacaga tttaataaat
    14341 taccacattt taagaatatt attaatctgt aatatatctt taacaatctc aggttaaaaa
    14401 ctttcctgtt ttcaacggga ctctcccgct gaatattcgc gcgttaatta aaatcaggaa
    14461 tgaaaatgaa aaagagcact ctggcattag tggtgatggg cattgtggca tctgtatccg
    14521 tacaggccgc agaaatatat aacaaagacg gtaataaact ggatgtctat ggcaaagtta
    14581 aagccatgca ttatatgagt gataatgaca gtaaagatgg cgaccagagt tatatccgtt
    14641 ttggttttaa aggcgaaaca caaattaacg atcaattgac cggttatggt cgctgggaag
    14701 cagagtttgc cggtaataaa gcagagagtg atactgcaca gcaaaaaaca cgtctcgctt
    14761 ttgccgggtt gaaatataaa gatttgggtt ctttcgatta tggtcgtaac ctgggcgcgt
    14821 tgtatgacgt ggaagcctgg accgatatgt tcccggaatt tggtggcgac tcctcggcgc
    14881 agaccgacaa ctttatgacc aaacgcgcca gcggtctggc gacgtatcgg aacaccgact
    14941 tcttcggcgt tatcgatggc ctgaacttaa ccctgcaata tcaagggaaa aacgaaaacc
    15001 gcgacgttaa aaagcaaaac ggcgatggct tcggcacgtc attgacatat gactttggtg
    15061 gcagcgattt cgccatcagt ggagcctata ccaactctga tcgcaccaac gagcagaacc
    15121 tgcaaagccg tggcacaggc aagcgtgcag aagcatgggc aacaggtctg aaatacgatg
    15181 ccaataatat ttatctggca actttctatt ctgaaacacg caaaatgacg ccaataactg
    15241 gcggctttgc caataagacg cagaactttg aagcggtcgc tcaataccag tttgactttg
    15301 gtctgcgtcc atcgctgggt tatgtcttat cgaaagggaa agatattgag ggcatcggtg
    15361 atgaagatct ggtcaattat gtcgatgttg gtgctacgta ttatttcaac aaaaatatgt
    15421 cagcgtttgt tgattataaa atcaaccaac tggaaagcga taacaaatta aatattaata
    15481 atgatgatat tgtcgcggtt gggatgacgt atcagtttta atgaatattg ccggatgtga
    15541 tgcatccggc acatttcatt cacgccgtta acttcaccgg ttcatcacga aaatcatctg
    15601 ccggttccag cttcagattc agcgtcgcca gcagctcacg cagcttctcg tgaaattcac
    15661 gcagggtgtg ctccagtcgt tcaaccactt cggcatcttt tatctgaaca ctcgtccagt
    15721 cgcctgcttt atcgaacaga ccaaactggt aactgtaggt aaaacgggat tcctgcgctt
    15781 ccagttccat ccaccagccc cagaactcac gcacttccgg tgccggtttc acgttgacgc
    15841 atacagccag acaatcgaaa aagaatcgat tatcttcgca cttaccttca cgaatatacg
    15901 ggcctagtgc ggtaaatttt ttgatcaatc tgctcttcgg gtgtccactc ggtaacgtca
    15961 ttgcaatctc ctgttgtgat gcaactgttt taccaaattg gtaaaattta gcaaattttt
    16021 aacacaaacg tttttcgatc cagtctgtga tttcctgaag gcctttgtca aaattccgat
    16081 acaccgggtt aaatgggatc tctaataatt taccgtcagc agatgacgac gtgattaagc
    16141 gtgagtcctc ttccgggctg aacggatcgt tcttccagta gcctgataac attggcgttg
    16201 ggcagcgacg tcccagcaat ccttgcactt ttaatgaata gcgattcaac tccactcgca
    16261 acgcttcatc ggaagcatca tgcatcccta aacgactggc cagaacgtca agatacattt
    16321 ccggcacctg ttgctggcac ttaaaatcac tcaacagggt atgaactacc ggaccaagac
    16381 aggcaaccac tttcagacgc ggcgattcaa ggtatgccag acgcacggca acgttagcgc
    16441 cgaaacggaa accaaaggcc gcgacgcgag tgtgatccac ccacggtacg ttaggcagcg
    16501 cctttaaaac gtgctgatgc aacaggctgg agtcctgggt gagcttccat tttgaagaaa
    16561 agcccaccga cggcatatca atagtcagca tagcaatgcc gcgcggcgca aaataacgtt
    16621 catacaggct gtaatagtcc gtctgcatcg catccagacc accacacatt aatactgtcg
    16681 ggaacgggcc atcgcctttc ggcatatgca aaaagccggt gatgggcgca ccgccgggta
    16741 cggtaaactc catctgccgc atcgtgcccg gcagacgctg agcggcctct tcataggcgc
    16801 ggtttgacaa agcctgcgct tgctcggcca ggtcatctcc tttcagatga ggataggcag
    16861 caatgttgta caacgtagcc gcatgcagcc agtatcgacc gctgagttgc ggatcttctt
    16921 ccgcacaggc tttttgttgc cacaccatcg cctgggtagc ccactcataa atccagttgc
    16981 cgccgcggta gccaatcacc gtatcgtata aatcatcgtc ggtacgttcg gcatcgctca
    17041 tcacaatacg cgcctggacg tcgaggattt cacgggggtc aatgccgcgc cagatccaca
    17101 tcagacggtt aatcatgcga taccagtgag ggatggtttt accatcaagg gccgactgca
    17161 caggcagttg tgcgccgtga ctaaagcggc ggactagcgt cgaggtttca ggatgtttaa
    17221 agcggggttt gaacagggtt tcgctcaggt ttgcctgtgt catggatgca gcctccagaa
    17281 tacttactgg aaactattgt aacccgcctg aagttaaaaa gaacaacgcc cggcagtgcc
    17341 aggcgttgaa aagattagcg accggagatt ggcggcacga atacgacgcc catatcccac
    17401 ggctgttcga tccaggtatt ttgcgggata tcaacaacat aatcatcaac cagcggacga
    17461 ccagccggtt ttgcgaagat ggtgacaaag tgcgcttttg gatacatttc acgaatcgca
    17521 accgcagtac caccggtatc taccaggtca tcaataacga tgaagccttc gccatcgcct
    17581 tctgcgcgtt tcagcacttt aagctcgcgc tggttgtcgt gatcgtagct ggaaatacaa
    17641 acggtatcga catgacgaat acccagttca cgcgccagta acgcacccgg taccagaccg
    17701 ccacggctta cggcaataat gcctttccat tgttcagaag gcatcagtcg gcttgcgagt
    17761 ttacgtgcat ggatctgcaa catgtcccag gtgacgatgt atttttcgct catgtgaagt
    17821 gtcccagcct gtttatctac ggcttaaaaa gtgttcgagg ggaaaatagg ttgcgcgaga
    17881 ttatagagat ctggcgcact aaaaaccagt atttcacatg agtccgcgtc tttttacgca
    17941 ctgcctctcc ctgacgcggg ataaagtggt attctcaaac atatctcgca agcctgtctt
    18001 gtgttgacaa cattttctgc taaccctgtg acctgcaata ctgttttgcg ggtgatcgac
    18061 aaggagactt aacgtgtctg aactgtctca attatctcca cagccgctgt gggatatttt
    18121 tgccaaaatc tgttctattc ctcacccgtc ctatcatgaa gagcaactcg ctgaatacat
    18181 tgttggttgg gcaaaagaga aaggtttcca tgtcgaacgc gatcaggtag gtaatatcct
    18241 gattcgtaaa cctgctaccg caggtatgga aaatcgtaaa ccggtcgtct tgcaggccca
    18301 cctcgatatg gtgccgcaaa aaaataacga caccgtgcat gacttcacca aagatcctat
    18361 ccagccttat attgatggcg aatgggttaa agcacgcggc accacgctgg gtgcggataa
    18421 cggcattggt atggcctctg cactggcggt tctggctgac gaaaacgtgg ttcacggccc
    18481 gctggaagtg ctgctgacca tgaccgaaga agccggtatg gacggtgctt ttggcttaca
    18541 gagcaactgg ttgcaggctg atattctgat taacaccgac tccgaagaag aaggtgaaat
    18601 ctacatgggt tgtgcgggag gtatcgactt cacctccaac ctgcatttag atcgtgaagc
    18661 ggttccagct ggctttgaaa ccttcaagtt aaccttaaaa ggcctgaaag gcggtcactc
    18721 cggcggtgaa atccacgttg gcctgggtaa tgccaacaaa ctgctggtgc gcttcctggc
    18781 gggtcatgca gaagaactgg acctgcgcct tatcgatttc aacggcggca cactgcgtaa
    18841 cgccatcccg cgtgaagcct ttgcgaccat tgctgtcgca gctgataaag tcgacgcttt
    18901 gaaatctctg gtgaatacct atcaggagat cctgaaaaac gagctggcag agaaagagaa
    18961 aaatctggcc ttgttgctgg actctgttgc gaacgataaa gctgccctga ttgcgaaatc
    19021 tcgcgatacc tttattcgtc tgctgaacgc caccccgaac ggtgtgatcc gcaactccga
    19081 cgtggcaaaa ggtgtggtcg aaacctccct gaacgtcggt gtggtgacca tgactgacaa
    19141 taacgtagaa attcactgcc tgatccgttc tctgatcgac agcggtaaag actacgtggt
    19201 gagcatgctg gattcgctgg gtaaactggc tggcgcgaaa accgaagcga aaggcgcata
    19261 tcctggctgg cagccggacg ctaattctcc ggtgatgcat ctggtacgtg aaacctatca
    19321 gcgcctgttc aacaagacgc cgaacatcca gattatccac gcgggcctgg aatgtggtct
    19381 gttcaaaaaa ccgtatccgg aaatggacat ggtttctatc gggccaacca tcaccggtcc
    19441 acactctccg gatgagcaag ttcacatcga aagcgtaggt cattactgga cactactgac
    19501 tgaactgctg aaagaaattc cggcgaagta attatttgat ttgctgccgg atggcgttta
    19561 atcgccttcc ggcagtttca tccttcatta tccttcgata aaagccatcc ctgtgaacgt
    19621 tcgtcgcggg ttgccacgtt caatctggtg atggaacatt cgccgctgcg atttcagcgc
    19681 cgcgctattt tcctgttgct gttgttccag cttccaggca atcagcaatc gcgccagccg
    19741 cttgttagca tgcccagaaa ccagtttttg cgcccatgat gaggccgata cggactcgga
    19801 caaatacact gaatagtgcc gcaccaactt tcgcttaatg cccatgcatt atcgccatcc
    19861 agagaaatca gcgccgaacg cagcgtgtca gagtagcggc ccgtttctgt ttccagcacc
    19921 gttaccgcga cgtcttgccg ggtaaaaacg accaacagag aagaacgctg cgccaatcgg
    19981 gtagccgcgt ccgggatgta aatctggcat ccccacaacg cgctgcatgt tgggtaaatt
    20041 tgccgtggtg tgtaattgtt ggatcgctaa actctcgatc cacaggtcat cagatgcgat
    20101 ggtaaatacc gcatcggata agggacgaat atatttgccc atgtatcatt ccatttatac
    20161 tcgtcatact tcaagttgca tgtactgcga ccgcgttcgt tcaccccagt cacttacttt
    20221 agtaagctcc tggggattca ctcgcttgtc gccttcctgc aactcgaatt atttagagta
    20281 tataaatata aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
    20341 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
    20401 ttgtggaata agaaaacgcg cacataccag gcgggtgtta ttttcattgc aagctggatt
    20461 taatgttgcg gtttatatcg catagaaaaa ttagtaaacc acgctcccct gcattcaacg
    20521 tgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctg
    20581 gcgtccaccg taagttcgga ttcagactta atcaaaggtt ttaacaaggc gctggcaacc
    20641 ccgcggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
    20701 ataaagccga ccagttttgc attaatgact gcaacccgca cttgtgattt cgcgagtttc
    20761 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtgg tgaataatgc
    20821 tgactggcgg tcatcgtaac cgctctaagg aaaatagcgc atagttgctg aaaatcgcca
    20881 ggctgatagt ctcttatttg tatgttattc ataatcccaa caccagttgt ctttccattt
    20941 gcgggtcaag taacgtcaca tgcagcccca ccagtcgcac accgcgcccg ccgcggcgtt
    21001 catcccaggt tttacgcgca gtggcgatta gatcagcttt attcagccgt ggccagacgt
    21061 gctcctgagt agtttgctga aaatcgtcga actttaattt cactccctga cgggcgatca
    21121 gtaaatcagg tttcaccttt gccagacggc gttcaagttc aggatacagc cgctcgataa
    21181 tcgcttcaca ttcagaccag tgatgaatat cttccgccat cgtgcgttcc acgccgacgg
    21241 attttcgcaa ccgttcactg ttaacgtcgc gctcgtcaat cccctgacta cgctcccaca
    21301 aaatgcggcc aaatttgcca aagcgtttaa gcagaatcac cagatcacac ttttgcacat
    21361 caccgcaggt ccgcagcccc atcgcttcca gttttgccgc cgagactttg ccgacgccgg
    21421 gaatttttgc cagcggtaag gtttgcagaa atgccggaac ttctgccggc gtaatcacaa
    21481 actgaccgtt gggtttattc atgtcggagg cgattttggc gagaaacttt actggtgcca
    21541 cgcccgcaga tgccgtcagt tgcagctcgt tgaagatcgt ctggcggatt tcctgggcga
    21601 tgagggtcgc agaaccgtgg caatggacgc tatcagtgac atcgagatag gcttcatcca
    21661 gtgacaacgg ttcaatacgc gaggtgtagc gcgagaagat ttcacggata tgatttgagg
    21721 cttctttgta ggcgtcaaag cgccccggaa gcaaggtgag atgcgggcat aatttgagcg
    21781 ccatccctgt cggcatagcg ctacgtacgc caaatttacg cgcgggataa ttggcggtgc
    21841 tgattacccc ccgtcgttcg cggctgccgc caatagcaat agggatatcg cgcagggcgg
    21901 gattgtcgcg catctccacc gctgcgaaaa agcagtccat atccacatga atgattttac
    21961 gcattgctca cctctcaaca ctggtaaagt atacagtgat ttcagggttt gagaaatgcg
    22021 taaagattca gcatttaacg cgctcgctgc gaaagcacct gctgcttatc cagcttttgc
    22081 accagcggct gcaccacttt atccccatgc tgaccaaagt attgatacag cgtatctgac
    22141 gcacttttgg tcagcaccat aatctcaatg cgccggttgc ccgcgctttg cggatttttg
    22201 gcatccagca gcatctggtc cgccattgcg cttacctgca tcactttatt ttccggcatt
    22261 ccggcctctt ccagcacccg acgagccgaa agcgcgcgat cgcccgaaag gttccagttg
    22321 ttgtagatat tgtttttgta ggccatcgca tcggtatgcc cggtaataat aattttgtta
    22381 tcgagcgagt cgaacactgg cgcgagctcc accagcagcg ttttaaagaa cggcataatc
    22441 ttcgcgctgc cgcgttcaaa catattgcgg ttctggtcat ctttaatcag cacgcgcagt
    22501 ccctggggaa caatctccat ctccagattc gcttccatat gtgcatcacg ggcaatggtg
    22561 ttgatactgg tcgccagctc gccaagctcg gtggcggatt tcttcttcag cagcgccgtt
    22621 ttctcgttga cgtcgcgcgc ttttttctcc gtctcttccg gcaccgccac ggtggcaggt
    22681 tttggcggat gagacgggct gattttattt aacggcgaca acccaccacc gttaaaaatc
    22741 gactgcccgt gcagcgcggc gataatgctt tcacgctcgg atttactgac gctattgaca
    22801 atcaacttta tcgtcgtcac caaaggggcc gagcgtattt ccgaggtttc cgcccgcttt
    22861 accttagacg cgatgcccgg taagcagatg gcgattgacg ccgatcttaa cgccgggttg
    22921 atcaaccagg cgcaggcgca aacccggcgt aaagatgttg ccagcgaggc cgatttctac
    22981 ggcgcaatgg acggggcatc gaagtttgtg cgcggggacg ccatcgccgg gatgatgatt
    23041 ctggcgatca acctgatcgg cggcgtctgt atcgggatct tcaaatacaa cctgagcgcc
    23101 gacgccgcct tccagcaata tgtgctgatg accatcggcg acgggctggt ggcgcagatc
    23161 ccttccctgc tgctctccac cgcggcggcg attatcgtca cccgcgtcag cgacaacggc
    23221 gatatcgccc atgacgtgcg ccaccagctg ctggcaagcc cgtcggtgct ctacaccgcc
    23281 accggaatta tgtttgtact ggcggtggtg ccgggaatgc cgcacctgcc gtttttgctg
    23341 ttcagcgccc tgcttggctt taccggctgg cggatgagca aacgcccgca ggcggcggag
    23401 gcggaggaga aaagtcttga aacgctaacc cgcaccatca ctgaaaccag cgagcaacag
    23461 gtcagttggg aaaccattcc gctgatcgag cctatcagct taagcctcgg ctacaagctg
    23521 gtggcgctgg tcgataaagc gcaggggaac ccactcaccc agcggattcg cggcgtgcgg
    23581 caggtgatat ccgacggcaa cggcgtgctg ctgccggaga tccgtattcg ggaaaacttc
    23641 cgcctcaagc ccagccagta cgccatattc atcaacggca ttaaggccga tgaagcggat
    23701 attccggcag ataaactgat ggccctgccc tccagcgaaa cctacggcga gattgacggc
    23761 gtactgggga atgacccggc atatgggatg ccggtcacct ggattcagcc tgcgcagaaa
    23821 gcgaaggcgc tgaatatggg gtatcaggtg atcgacagcg ccagcgtgat tgccacccat
    23881 gtgaataaga ttgtgcgcag ctatattcct gatttattta actatgacga tattacgcag
    23941 ttgcataacc gcctgtcgtc gatggcaccg cgactggcgg aagacttaag cgcagcgctc
    24001 aattacagcc agttgctgaa agtgtatcgc gcgctgctga ccgaaggcgt ttccctgcgc
    24061 gatattgtca ccatcgccac cgtgctggtc gccagtagtg cggtgactaa agatcatatc
    24121 ctgctggcgg cagatgtgcg cctggcgctg cggcgcagta tcacccatcc gttcgttcgc
    24181 aagcaggagc tgacggtgta tacgctgaat aatgagctgg aaaatctgct gaccaacgtg
    24241 gtgaatcagg cgcaacaggc cggcaaagtg atgctcgaca gcgtgccggt ggacccgaat
    24301 atgcttaacc agttccagag tacgatgcca caggtgaagg agcagatgaa agcggcgggg
    24361 aaagacccgg tgctgctggt gccgccgcaa ctgcgtccat tgctggcgcg ctatgcaagg
    24421 ttgtttgcgc cggggctgca tgtgctgtcg tataacgaag tgccggatga gctggagttg
    24481 aagattatgg gggcgttgat gtaagaatcc ggcatcggtg cacaattgcc ggatgcggcg
    24541 tgaacgcctt atccggccta ccgttaaggc atagatttgt aggcatgata agacgcgcta
    24601 gcgtcgcatc aggcatctgc gcacaaccgc cggatgcggc gtgaacgcct tatccggcct
    24661 accgttaagg cacagatttg taggccggat aagcgtaagc gcatccggca ccggcgcaca
    24721 attataaaat aatacgctcc ccggcactaa aatccgttac gtcccccgcc caatcgatgg
    24781 gatataaacc tttcgcgaca tcgcgatgga acgttgagaa tggccaatca ctcacctgct
    24841 ttacccagcc atgtttcact ggatttatgt aaatataatc aacatgatga cgataatctt
    24901 tggtattgcg aatggcatgc tcccaaaaac gcggttgcca catattttgc aatccacaag
    24961 catgggtgaa ctgtttttta atctcccgcc agcgagagga aaaatcatca tcgctttcgg
    25021 gtaatgtcca gatacagtgc atatgttccg gcaaaacgac ccaggcgtta atttcaaaag
    25081 gtctggcctg cttaacttta ataatggcgt tacggagtgt ctggtactgg gtggtcaaaa
    25141 gttggcttcg acgattccgt aaatttaccg tgaaaaacca tgttcccccc ttgatgtaat
    25201 aacgacgata ttcagacatg gcgactcctt tttctcaacg ataagaagaa aaggaaaagt
    25261 cgacaatgaa gttgtgttat atcgggaaag cagctcgcaa aaaattcgca gactcgtagg
    25321 catgataaga cgcggcagcg tcgcatcagg catcggtgca ctattgccgg atgcggcgtg
    25381 aacgccttat ccgtcctaca aaatcgtttc ctccgtcaaa atcctgcgcg cgcctaaaaa
    25441 atggtcctgc cagaaaggtt cggctaaccg acttacccga atggtttccc cggtgcgcgg
    25501 tgactcgata aactgaccgt ctccaagata tacgcccata tggtcggcta tctcacgact
    25561 gtggatatgg aaaaacagca aatctccccg gcgcaggtcg ttattcgcca caatcgttgc
    25621 ccgatggtag tgatacatct cattggccgt acgcgggagc ttcgcctcga ggatcttgtt
    25681 gtaggcataa aaaaccaacc cgctacagtc gaagccttga tcgggccgcg tgccgcccca
    25741 gacgtaaggc ttaccaagct gctgctccag gcggtgaatc gccacttcgg tgatgttgtg
    25801 tagatggtcg ctgcttaaga aatggttgtt ctccgccagc gcctgccagc gtctgtcact
    25861 cgccttgagc ggcgcaggaa accattctgg atgctgtttt atctgctccc gattatgctg
    25921 gcgcagcgcc cttctgcttt tggcattgcc ttccacaata tagctggcct gctttttcag
    25981 atgggtttgg tactgtttta ataaacgcgc acggttttgc atccgctggc gggcggcgta
    26041 gctgaaagag atatgtgatg tggtggcgct ggcgagtaaa gggagcaaaa cgcataacga
    26101 aaacacgcct ggatgcagaa atctgccgtg caagaacgaa gacatgaagg acataaacca
    26161 gctgaatgaa ggtgagaatt ttaaaagacc ggaggagtaa acgggattat taattgctta
    26221 tttgcatatt actattgcgg tttcataatc ttaggaaata agaaataaat ctacaatcca
    26281 tgcgcttttc ggactcttga aaactgcccc tgtgctaaat ttgtaaaccc tgcgccgaca
    26341 ggcttgcgta aaactctttt ggtgataatg tgagccttcc aaatccagac tattccgatt
    26401 tgggattcat tttgccgtcc tggcgtgtgt aaccgtttta tcaaggaata agcagtatgc
    26461 gtaaaatcgc attaattctt gcgatgttgt tgatcccgtg cgtttcgttt gccggtttgc
    26521 tgggcagcag tagctcgacc acgccagtga gcaaagagta taagcagcag ttgatgggat
    26581 cccctgtcta catccagatc ttcaaggaag aacgtacgct cgatctctac gtcaaaatgg
    26641 gcgagcaata tcaactgctc gacagctata aaatctgtaa atattccggc ggcttagggc
    26701 caaaacagcg tcagggcgat ttcaaaagcc cggaagggtt ttatagcgtc cagcgtaatc
    26761 agttaaaacc agacagccgt tactacaaag cgattaatat tggattccca aacgcctatg
    26821 accgtgcgca tggttatgaa gggaaatacc tgatgattca cggcgattgt gtttccatcg
    26881 gctgctacgc gatgaccaat cagggtattg atgagatctt ccagttcgtt actggtgcgc
    26941 tggtgtttgg tcagccgagc gtgcaagtga gtatttaccc gttccgtatg accgacgcca
    27001 atatgaagcg ccataaatat tccaacttta aggacttctg ggagcaactg aagccaggct
    27061 acgactactt tgagcaaacc cgtaagccac cgaccgtttc tgtggtcaat ggccgttacg
    27121 tggtcagcaa gccgttaagc cacgaagttg tgcagccaca actggcatca aactacacgc
    27181 tccccgaggc aaaataagcg ccattcacct ggcataatct tttgccaggt ttcattgccc
    27241 gtcagcggct gtgtcgcaat caccgtgacc acatcattcg gtgtggtctg cgagctgaag
    27301 tcgatttcca catcctgatc cagcaacgtt gccacgccaa acggcgcgcg gcgggtgatc
    27361 cagtgtaaat tagtcgagca atacgccatt acatagcgcc cgtcagaaag cagcatgttg
    27421 aaaacgccct tctgccgcag ttcatccgcc agtgaggcga tatatttaaa cactgccgcc
    27481 atgttgcccg gtgtgcgcgg gtaacgctgc gttaatttat gcaggagcca gcaaaaggct
    27541 ttttcgctat cggtttcacc gaccgggcgg aagttgccgg tttccagtga tttgtagccc
    27601 gtcagttgtc cattatgggc ataagtccag ttacgccccc ataactcgcg ggtaaacggg
    27661 tgagtatttt ccagcgccac ctcgccccga ttagcctggc gaatatgagc caccaccgaa
    27721 caggatttta tcgggtagtc ctggacaagt ttggcgatgg gggaattaaa gctgggttgc
    27781 ggatctttaa atgtgcgaca gcctttacct tcgtaaaagg taatgcccca gccatcttta
    27841 tgtggcccgg ttccaccacc acgctgtaca agtccggtga aactaaagca gatatcggta
    27901 gggacgttgg cgctcatccc gagcaattcg cacatgggta acctccacaa cagcgggagc
    27961 gcatggcccc cggcgaagat tgattaaaaa gtacaaaggt tcaggctgtt accctcaccc
    28021 cggccctctc cctgaaaggg agaaaagacc ttacttaacc atctcttttt caattaactg
    28081 gatcaggata tggatcactt taatgtggat ctcctgaatg cggtcggcat aaccaaagtg
    28141 cggtacgcga atttcgatat ccgctgtgcc agccattttg ccgccgtctt taccggtcag
    28201 ggtgatcact ttcatcccct tctcacgcgc cgccgcgatc gctttgatca cgtttgcaga
    28261 gttaccggag gtggagatcc ccagcagtac atcgccttcg cgacctaccg cttcaacgta
    28321 acgggagaaa atatcattga aaccaaaatc gttaccgacg caggaaatat ggctgacgtc
    28381 agaaatggca atcgccgggt agcccggacg gttttcacgg tagcgaccgg tcaactcttc
    28441 ggcaaagtgc atagcgtcgc aatgggagcc gccgttgccg caggaaagca ctttgccacc
    28501 ggctttaaag ctgtctgcta acaggaccgc cgcgcgctga atggcgtgaa tattggcgtc
    28561 atcttttaaa aaattagcca gcgtttccgc cgcttcgttc agttcgttac gaataagatc
    28621 ctggtacatg aggatatcct tcagcataaa tgtaatagac aaaatgcagt gtaccggata
    28681 ccgccaaaag cgagaagtac gggcaggtgc tatgaccagg actttttgac ctgaagtgcg
    28741 gataaaaaca gcaacaatgt gagctttgtt gtaattatat tgtaaacgta ttgctaaatg
    28801 tttttacatc cactacaacc atatcatcac aagtggtcag acctcctaca agtaaggggc
    28861 ttttcgttat gatgattttg agtattctcg ctacggttgt cctgctcggc gcgttgttct
    28921 atcaccgcgt gagcttattt atcagcagtc tgattttgct cgcctggaca gccgcactcg
    28981 gcgttgctgg tctgtggtcg gcgtgggtac tggtgcctct ggccattatc ctcgtgccat
    29041 ttaactttgc gcctatgcgt aagtcgatga tttccgcgcc ggtatttcgc ggtttccgta
    29101 aggtgatgcc gccgatgtcg cgcactgaga aagaagcgat tgatgcgggc accacctggt
    29161 gggaaggcga tttgttccag ggcaagccgg actggaaaaa gctgcataac tatccgcagc
    29221 cgcgcctgac cgcagaagag caagcgtttc tcgacggccc ggtagaagaa gcctgcagga
    29281 tggcgaatga tttccagatt acccatgagc tggcggattt gccgccggag ctgtgggcgt
    29341 atcttaaaga gcatcgcttc ttcgcgatga tcatcaaaaa agagtacggc gggctggagt
    29401 tctcggctta tgcccagtct cgcgtgctgc aaaaactctc cggagtgagc gggatcctgg
    29461 cgattaccgt cggcgtgcca aactcattag gcccgggtga gctactgcaa cattacggca
    29521 ctgacgagca gaaagatcac tatctgccgc gtctggcgcg tggtcaggag atcccttgct
    29581 ttgcactgac cagccccgaa gcgggttccg atgcgggcgc gattccggat accgggattg
    29641 tttgcatggg cgagtggcag ggccagcagg tgctggggat gcgactgacc tggaacaaac
    29701 gctacattac gctcgcgccg attgcgaccg tgcttggtct ggcgtttaaa ctctccgacc
    29761 cggaaaaact gctgggtggc gcagaagatt taggtattac ctgtgcgctg atcccgacca
    29821 ccacgccggg cgtggaaatt ggtcgtcgcc acttcccgct gaacgtgccg ttccagaacg
    29881 gaccgacgcg cggtaaagat gtcttcgtac cgatcgatta cattatcggc ggacccaaaa
    29941 tggccgggca aggctggcgg atgctggtgg agtgcctctc ggtaggccgc ggtatcaccc
    30001 tgccttccaa ctcaaccggc ggcgtgaaat cggtagcgct ggcaacgggt gcgtatgcgc
    30061 acatccgccg tcagttcaaa atctctattg gtaagatgga agggattgaa gagccgctgg
    30121 cgcgtattgc cggaaacgct tatgtaatgg atgctgcggc atcgctgatt acctacggca
    30181 ttatgctcgg tgaaaaacct gccgtgctgt cggctatcgt taagtatcac tgtacccacc
    30241 gtgggcagca gtcgattatt gatgcgatgg atatcactgg tggcaaaggc attatgctcg
    30301 ggcaaagtaa cttcctggcc cgtgcttacc agggcgcacc gattgccatc accgttgaag
    30361 gggctaacat tctgacccgc agcatgatga tcttcggaca aggagcgatt cgttgccatc
    30421 cgtacgtgct ggaagagatg gaagcggcga agaacaatga cgtaaatgcg ttcgataaac
    30481 tgctgttcaa acatatcggt cacgtcggta gcaacaaagt tcgcagcttc tggctgggcc
    30541 tgacgcgcgg tttaaccagc agcacgccaa ccggcgatgc cactaaacgc tactatcagc
    30601 acctgaaccg cctgagcgcc aacctcgccc tgctttctga tgtctcgatg gcggtgctgg
    30661 gcggcagcct gaaacgtcgt gagcgtatct cggcacgtct gggggatatt ttaagtcagc
    30721 tttacctcgc ctctgccgtt ttgaagcgtt atgacgacga aggccgcaac gaagccgacc
    30781 tgccgctggt gcactggggc gtacaggatg cgctgtatca ggcagaacag gcgatggacg
    30841 atttactgca aaacttcccg aaccgcgtgg ttgccgggct gctgaatgtg gtgatcttcc
    30901 cgaccggacg tcattatctg gcaccttctg acaagctgga tcataaggtg gcgaagattt
    30961 tacaagtgcc gaacgccacc cgttcccgca ttggtcgcgg tcagtacctg acgccgagcg
    31021 agcataaccc ggttggttta ctggaagagg cgctggtgga tgtgattgcc gccgacccga
    31081 ttcatcagcg gatctgtaaa gagctgggta aaaacctgcc gtttacccgt ctggatgaac
    31141 tggcgcacaa cgcgctggcg aaggggctga ttgataaaga tgaagccgct attctggtga
    31201 aagctgaaga aagccgtctg cgcagtatta acgttgatga ctttgatccg gaagagctgg
    31261 cgacgaagcc ggtaaagttg ccggagaaag tgcggaaagt tgaagccgcg taattgaata
    31321 acggagccga aaggctccgt ttctttatcc gctaattatt taaaattaaa gccatccgga
    31381 tggttttcca ggctgccggt caacgccgcg aacagcaccg ttttaccatc aatagaaagc
    31441 gcatcgttca cattcaacca ggtgagtttc tcttgcgacg ttttctcatc aatagtcgag
    31501 aacagccccg tcatctgatt agatttctcg gaccacatca cagcgatacg ttgcgagcca
    31561 cagtcatgcg gtttgcacgc gctcatcacc tgatacgtct catctcccaa tgtcacggtt
    31621 tgtgcgggag tataagtacc gcctttcatc acccaggcag gcagcttatg cccttgcacc
    31681 atctgattaa acgccgcttt ggtggtttcg ccctttgcaa gactgctaat ggttaaatca
    31741 tcctgcgcca ttgcactggt ggcgatgacc agagcggcga ctgtcgttat tgccttaaac
    31801 atcattcctc ccgggcttat cctgcccatg tgattaactc cctaaagcct ggcgataaca
    31861 cgataatttg caaatcagat atttccaata tcagcatttg ttatcaattt attctgcgct
    31921 aaggcaactg cctgataagg tgttagagtt ataaaaccct tcgcataaag gagttgtaca
    31981 ccgtgcctgg tttgaagatt acgcttttgc agcaaccact ggtgtggatg gatggtcctg
    32041 ccaacctgcg ccattttgat cgtcaactgg aaggtattac cgggcgcgat gtgatcgttc
    32101 taccggagat gtttaccagc ggctttgcca tggaagcggc agcttcgtcg ctagcacaag
    32161 atgacgtagt gaactggatg accgctaaag cgcagcagtg caatgcgctg attgcaggca
    32221 gtgttgcatt acaaacggag tctggttcgg ttaaccgctt tttgctggtt gagccgggcg
    32281 gcacggtaca tttttatgat aagcgtcatc tgttccgcat ggcagatgag catctgcatt
    32341 ataaagcggg caatgagcga gtgattgtgg aatggcgcgg ctggcgtatt ttgccgctgg
    32401 tgtgctacga cttacgtttt ccggtgtggt cgcgcaatct caacgattat gacctcgccc
    32461 tgtacgtcgc caactggcct gctccgcgct ctctgcactg gcaggcattg ctgacagccc
    32521 gcgcgattga gaatcaagcg tatgtggcgg gatgcaatcg cgtcggcagc gatggcaacg
    32581 gctgccatta tcgcggtgac agccgggtga ttaatccgca aggcgagatt atcgctactg
    32641 ccgacgcgca tcaggcaacg cgcattgatg cggagctgtc gatgatggca ttgcgggaat
    32701 atagagaaaa gtttccggca tggcgggatg cggatgagtt taggttgtgg tgagtggggg
    32761 ttgtgtttag ggctggggag agtcagggta agactacgaa agcccgctc
//
LOCUS       DAZGHF010000045        32197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGHF010000045 DAZGHF010000000
VERSION     DAZGHF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.045"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            226..3489
                     /locus_tag="ACHK3A_002193"
     CDS             226..3489
                     /locus_tag="ACHK3A_002193"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO8740265.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYINPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEANIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVIGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            3704..4042
                     /gene="symE"
                     /locus_tag="ACHK3A_002194"
     CDS             3704..4042
                     /gene="symE"
                     /locus_tag="ACHK3A_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO8740266.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVIEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQRQVQDF
                     IGVISSKTPR"
     gene            complement(4084..4137)
                     /locus_tag="ACHK3A_002195"
     CDS             complement(4084..4137)
                     /locus_tag="ACHK3A_002195"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO8740267.1"
                     /translation="MSDERLRDIGLRREDVE"
     gene            complement(4126..4281)
                     /locus_tag="ACHK3A_002196"
                     /pseudo
     CDS             complement(4126..4281)
                     /locus_tag="ACHK3A_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001684533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            4424..5836
                     /gene="yjiR"
                     /locus_tag="ACHK3A_002197"
     CDS             4424..5836
                     /gene="yjiR"
                     /locus_tag="ACHK3A_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO8740268.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(6078..7022)
                     /locus_tag="ACHK3A_002198"
     CDS             complement(6078..7022)
                     /locus_tag="ACHK3A_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO8740269.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQQRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            7489..8721
                     /gene="mdtM"
                     /locus_tag="ACHK3A_002199"
                     /pseudo
     CDS             7489..8721
                     /gene="mdtM"
                     /locus_tag="ACHK3A_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
     gene            8762..10042
                     /gene="yjiN"
                     /locus_tag="ACHK3A_002200"
     CDS             8762..10042
                     /gene="yjiN"
                     /locus_tag="ACHK3A_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO8740270.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDAQDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            10158..11309
                     /gene="yjiM"
                     /locus_tag="ACHK3A_002201"
     CDS             10158..11309
                     /gene="yjiM"
                     /locus_tag="ACHK3A_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO8740271.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            11319..12086
                     /gene="yjiL"
                     /locus_tag="ACHK3A_002202"
     CDS             11319..12086
                     /gene="yjiL"
                     /locus_tag="ACHK3A_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO8740272.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTSFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            12083..12340
                     /gene="ytiA"
                     /locus_tag="ACHK3A_002203"
     CDS             12083..12340
                     /gene="ytiA"
                     /locus_tag="ACHK3A_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO8740273.1"
                     /translation="MKEHLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            12405..13265
                     /gene="yjiK"
                     /locus_tag="ACHK3A_002204"
     CDS             12405..13265
                     /gene="yjiK"
                     /locus_tag="ACHK3A_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO8740274.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            13333..14511
                     /gene="yjiJ"
                     /locus_tag="ACHK3A_002205"
     CDS             13333..14511
                     /gene="yjiJ"
                     /locus_tag="ACHK3A_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8740275.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMILIALTLLMPSKKHAIVPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(14524..15078)
                     /gene="kptA"
                     /locus_tag="ACHK3A_002206"
     CDS             complement(14524..15078)
                     /gene="kptA"
                     /locus_tag="ACHK3A_002206"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO8740276.1"
                     /translation="MAKYNDKELAETSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDIVVATSDKKRFSYSSDGTCIRAVQGHSTSQVAIGFIEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            15328..16011
                     /gene="yjiH"
                     /locus_tag="ACHK3A_002207"
     CDS             15328..16011
                     /gene="yjiH"
                     /locus_tag="ACHK3A_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO8740277.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            16008..16469
                     /gene="yjiG"
                     /locus_tag="ACHK3A_002208"
     CDS             16008..16469
                     /gene="yjiG"
                     /locus_tag="ACHK3A_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO8740278.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            16482..17654
                     /gene="iadA"
                     /locus_tag="ACHK3A_002209"
     CDS             16482..17654
                     /gene="iadA"
                     /locus_tag="ACHK3A_002209"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO8740279.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQMLVKDYSFSLSDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            17719..18630
                     /gene="hypT"
                     /locus_tag="ACHK3A_002210"
                     /gene_synonym="qseD"
     CDS             17719..18630
                     /gene="hypT"
                     /locus_tag="ACHK3A_002210"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO8740280.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNREELVIPIQAY
                     AYRMNTRMNPIAERFWRELRELEIEPG"
     gene            18637..19008
                     /locus_tag="ACHK3A_002211"
                     /pseudo
     CDS             18637..19008
                     /locus_tag="ACHK3A_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
     gene            complement(19056..19439)
                     /gene="iraD"
                     /locus_tag="ACHK3A_002212"
     CDS             complement(19056..19439)
                     /gene="iraD"
                     /locus_tag="ACHK3A_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO8740281.1"
                     /translation="MMRQSVQTVLPESTGHNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGNWHRTMPQ"
     gene            complement(19853..20626)
                     /gene="uxuR"
                     /locus_tag="ACHK3A_002213"
     CDS             complement(19853..20626)
                     /gene="uxuR"
                     /locus_tag="ACHK3A_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO8740282.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(20841..22301)
                     /gene="uxuB"
                     /locus_tag="ACHK3A_002214"
     CDS             complement(20841..22301)
                     /gene="uxuB"
                     /locus_tag="ACHK3A_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO8740283.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(22382..23566)
                     /gene="uxuA"
                     /locus_tag="ACHK3A_002215"
     CDS             complement(22382..23566)
                     /gene="uxuA"
                     /locus_tag="ACHK3A_002215"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO8740284.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIVGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            23906..25249
                     /gene="gntP"
                     /locus_tag="ACHK3A_002216"
     CDS             23906..25249
                     /gene="gntP"
                     /locus_tag="ACHK3A_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO8740285.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(25422..26324)
                     /gene="fimH"
                     /locus_tag="ACHK3A_002217"
     CDS             complement(25422..26324)
                     /gene="fimH"
                     /locus_tag="ACHK3A_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO8740286.1"
                     /translation="MKRVIYLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(26344..26847)
                     /gene="fimG"
                     /locus_tag="ACHK3A_002218"
     CDS             complement(26344..26847)
                     /gene="fimG"
                     /locus_tag="ACHK3A_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO8740287.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTAGFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(26860..27390)
                     /gene="fimF"
                     /locus_tag="ACHK3A_002219"
     CDS             complement(26860..27390)
                     /gene="fimF"
                     /locus_tag="ACHK3A_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO8740288.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAVKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(27400..30036)
                     /gene="fimD"
                     /locus_tag="ACHK3A_002220"
     CDS             complement(27400..30036)
                     /gene="fimD"
                     /locus_tag="ACHK3A_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO8740289.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(30103..30828)
                     /gene="fimC"
                     /locus_tag="ACHK3A_002221"
     CDS             complement(30103..30828)
                     /gene="fimC"
                     /locus_tag="ACHK3A_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO8740290.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(30865..31404)
                     /gene="fimI"
                     /locus_tag="ACHK3A_002222"
     CDS             complement(30865..31404)
                     /gene="fimI"
                     /locus_tag="ACHK3A_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8740291.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     VEAGDKQMTVNMGKISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(31469..32023)
                     /gene="fimA"
                     /locus_tag="ACHK3A_002223"
     CDS             complement(31469..32023)
                     /gene="fimA"
                     /locus_tag="ACHK3A_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO8740292.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTA
                     IDGTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         7692 a         8244 c         7992 g         8269 t
ORIGIN      
        1 aatggttttc taattcgtct gaaaataata ccctaacagc cctccgcaat accctgctcc
       61 ccaaactcat ctccggcgag ctatccgtgg aggatctccc ggatcacatc aaacaaaccg
      121 aagccgcata acgcattttg cccctgtaaa atcaggggct ttctggtaag gttttctact
      181 gatacgggaa tgcttaccag aaattagcca gggttggagc acgatatgag tctctctttc
      241 agtgaagcaa aattagaaca agcgatcatt gaactgttac aggatcaggg atatcaacac
      301 ctgatcggcg ataatgtccc acgttcaagt ctcgatcagg tcattatcga agacgatctc
      361 cgtcattatt tagcggcacg ctaccagcct gatggcatta ctgaagaaga gattcagcga
      421 ctgatcaaac agttcaccac gcttccggcc tccgatcttt atgaaagcaa caaaacattt
      481 tgtcgctggc tggcaaatgg ttttctgttc aaacgcgacg atcggcaaca aaaagatctc
      541 tatattgaat tgctcgacac ccggcatcta cctgccgcac tgcgccagat atttgacgcc
      601 gaagatgtcc cgttgcaaca ggctgcggaa ctcccgcctt cctatattaa cccgccgctt
      661 aaccggatta agattgttaa ccagcttaaa atctccggca aagataatca gactcgtatt
      721 cctgacggca ttctctatat caacggtctg ccactggtcg tctttgaatt taaaagcgcg
      781 gtgcgcgagc aggaagccaa tataggcgat gcctggaagc agctctgtaa acgctaccgc
      841 cgggatattc cgcaactgtt tatctacaac gcgctctgca ttattagcga tggcgtaaat
      901 aaccggatgg gcaacctgtt tgcaccctat gaatattttt actcatggcg caaagtcacc
      961 ggtaatgaaa accgtgaaca ggacggaatt ccatcattgc actcaatgat tcaggggctg
     1021 tttcatccgg tacgtctgct ggatgtgatt aaaaacttta tctgcttccc ggataaagcc
     1081 aagcacgaag taaaaatttg ctgccgatat ccgcagtact atgccgcccg caaactctat
     1141 tacagcatca agcaagcgcg taaacctttc ggtagcggta aaggcggtac ctactttggc
     1201 gcaacgggct gcggtaaaag ctacaccatg caatttttaa cccgcttgtt aatgaaaagc
     1261 gtggagtttg ccagcccgac cattgttttg atcaccgacc gcaccgatct ggacgatcag
     1321 ctttctgcgc aaatgtgcaa cgccaaaaat tacattggcg acgacaccat cgttcctgtt
     1381 accagccgtg aagatttgcg taatcaactg gcgggacgca atagtggcgg tgtattcctg
     1441 acaacgatcc ataaattcac cgaagacacc gaactccttt ctgaacgcag caatatcatt
     1501 tgcatctcgg acgaagcaca tcgcagccag gttaacctcg accagaaagt catcgtcgat
     1561 aaagaaagcg gaaaagtgcg caaaacgtat ggctttgcga aatacctgca cgattcactg
     1621 ccaaatgcca cctatgttgg ctttaccggc acaccgattg acgcgacgct cgatgtcttc
     1681 ggtgaggtga tcgacagtta caccatgacc gaatccgttc aggatgaaat cactgtacgc
     1741 atcgtgtacg aaggccgtgc ggctaaagtg atcctggact ccagcaaact ggaagaagtc
     1801 gaaaagtatt acgaagagtg cgcaaacgca ggcaccaatg agtggcaaat cgacgaaagc
     1861 aagaaagcca cggctaccat gaatgcggtt ctgggtgatg aagatcggtt aaaagccctc
     1921 gcggaagatt ttgctaaaca ttatgaaaaa cgcgtagccg aaggttccac cgtaataggc
     1981 aaagccatgt ttgtttgtgc cagccgtgaa attgcctggg atttctaccg ccagcttaaa
     2041 gctattcgcc ctgcctggtt tgaagtgaag caagcacctg atggcgtcgt cctgacggaa
     2101 caggaacaaa aagagttacc gccttctgaa atggtgaaaa tggtcatgac gcgcggtaaa
     2161 gatgacgacg aggcgcttta tgatttactg ggcacgaaag aatatcgtaa agagctggat
     2221 aagcagttca aaaacgctaa atcgaatttc aaaattgcca ttgtcgttga tatgtggctg
     2281 accggttttg atgttcctga actggatacc atctatattg ataagccctt acaaaaacat
     2341 aaccttatcc agactatttc tcgcgttaac cgtaagctgg aaggcaaaag caaagggtta
     2401 gtggtggact acatcggcat taaaagtcag atgaaccagg cactggcaat gtattcccgc
     2461 attgatgcca ccaactttga agatattcag caatcggtaa tagaagttaa aaaccatctc
     2521 gatttgttgg ggcaagtctt ttacgaattt gacagtcggg attattttag tggtgagcca
     2581 caagcgcaat tatcctgcct caaccgcgcg gcggaatttg ttctgcgtac ccagaaagtt
     2641 gaacgtcgtt ttatgggact ggttaaacgc atgaaagccg cctacgacgt ctgctgcggc
     2701 agcgaagcat tatcacagac agaacgtgat catattcact attatcttgc tgttcgttca
     2761 attgttttca aactgacgaa aggtgacgca ccggatgtca cccagatgaa tgcacgcgtt
     2821 cgtgaaatga ttgcagaagc gctaaaagct gatggcgtag aagaaattta ttttcttggc
     2881 gataaaaaag cggaatccat cgatattttt gacgaagatt atctggcgcg aattaacaag
     2941 atcaaacttc cggcaacaaa gatccagtta ttacaaaaat tactggaaaa agcgatcagc
     3001 gacttcaaga aagtgaacca gttgcaaggg attaacttca cccgccgctt ccaggcgatt
     3061 atagatcgtt ataatgagcg gcgagaagat gatgtactca acggtgaaga attcgataca
     3121 ttcagtcagg aaatgaccga tattatctat gatattaaaa ccgaaatggg cacctgggcc
     3181 gatttaggca ttgatattga agaaaaagcc tttttcgaca ttcttgctca tatgcgcgat
     3241 aaatatcagt tcacctatga cgatgaaaaa atgctgtcac tggcaaaaga gatgaaaagc
     3301 gtggttgaca acacatcgaa atatcctgac tggagtaaac gcgatgatat taaagcgaaa
     3361 ctgaaagttg aacttattct gcttctacac aagcataagt tcccgccagt agcgaatgat
     3421 gatgtttata tgggtgtgct ggcacaggca gagaatttta aagaacatca tatgagtgct
     3481 ttaaattaat tatgaaggcc ggatacactt tgttatccgg ccattctgaa ttaacgttaa
     3541 tttctcttcc agatctttcc agtaacgctt ttattatttt ctatttttag ctggactttc
     3601 cctgtattta ctgatgatat atccaggtat ttagcgcggt gcgaatgtgc gccaacacac
     3661 ccgcaccgct catcacaatc cctatttcac gagaatagta atcatgactg acacgcattc
     3721 tattgcacaa ccgttcgaag cagaagtctc cccggcaaat aaccgtcaat taaccgtgag
     3781 ttatgcgagt cgctacccgg attacagccg tattcccgcc atcaccctga aaggtcagtg
     3841 gctggaaacc gccggttttg ccaccggcac ggcggtagat gtcaaagtga tagaaggctg
     3901 tattgtcctc accgcccaac cacccgccgc cgaagagagc gaactgatgc agtcactgcg
     3961 ccaggtgtgc aagctgtcgg cgcgtaaaca aaggcaggtg caggatttta ttggggtgat
     4021 tagtagcaaa acgccacgct aagtttctct ccccttcccc atacgggaag ggaaattgcc
     4081 ccctcactcc acatcctccc tgcgtaaccc gatatccctc aaccgttcat cactcatctg
     4141 ctgtacaccc gccgggcctg catttgccgc cgccaacgcc tcaccgcctg tcagagttgc
     4201 acaagtctga taaacggcgt tttggctttg ttttcgtgaa attccatctg ctgcctcctc
     4261 atctttcaga ggcttcatca tccgtcttct ggcagttcac aatacagatg caaatattag
     4321 ttttatttaa catacagaca ggttaaaaca gcatctgcat ccggtttttt agcctcatct
     4381 gtaccggttt ttctctctgt atggtccaaa taaggaatac agcatgacgc gttatcaaca
     4441 tctggcgact ctacttgccg agcggattga gcaagggctg tatcgtcacg gggagaaatt
     4501 gccgtcggtg cgcagcttaa gtcaggagca cggcgtcagt attagcaccg tgcagcaggc
     4561 gtaccagacg ctggagacga tgaagctcat cactccgcag ccgcgttcgg gttattttgt
     4621 cgcacaacgt aaagcccagc cgccagtacc gccgatgacg cgtccggtgc agcgcccggt
     4681 ggaaattacc cagtgggatc aagtgctgga tatgctggag gcgcatagcg acagttccat
     4741 tgttccgtta agtaaaagca cgccggatgt cgaaacgccc agcctgaaac cgctgtggcg
     4801 ggagctaagc cgggtggtgc agcataatct gcaaaccgtt ctcggttatg acttgttagc
     4861 cggtcagcga gtgttgcgag agcagattgc ccgcctgatg ctcgacagcg gctcagtggt
     4921 caccgctgat gacatcatta tcaccagcgg ctgccataac tcgatgtcgc tggcgttaat
     4981 ggcggtatgt aaaccgggcg atattgtcgc agtcgaatcc ccctgttatt acggttcaat
     5041 gcagatgctg cgcggtatgg gcgtgaaagt gattgaaatc ccaaccgatc cagaaactgg
     5101 catcagcgtt gaagcgctgg aactggcgct ggaacagtgg ccgattaaag gcatcattct
     5161 ggtgccaaac tgtaataatc cgctgggatt tattatgccg gacgcacgca aacgggccgt
     5221 tctctctctc gctcagcgtc atgatattgt gatttttgaa gatgatgtct acggcgaact
     5281 ggcgacggag tatccgcgcc cgcggaccat tcattcctgg gatatcgacg ggcgagtgct
     5341 gctgtgcagc tcgttcagta aaagtattgc accaggcctg cgcgtgggtt gggtcgcacc
     5401 ggggcgttat cacgataaac tgctgcatat gaaatatgcc atcagcagct ttaatgtacc
     5461 gtccacgcaa atggcggcgg caacgtttgt tctggaaggt cactatcatc gccatatccg
     5521 gcggatgcgg cagatctatc agcgcaattt ggcgctttat acctgctgga tacgggaata
     5581 ttttccctgc gaaatctgta ttacgcgccc gaaaggcgga tttttactgt ggatagaatt
     5641 gcctgaacag gtcgatatgg tttgcgttgc gcggcagctg tgccgcatga aaatccaggt
     5701 ggcggcaggc tcgattttct cagcttccgg caaataccgt aattgtctgc gcatcaactg
     5761 cgctttgccg ctcagcgaaa cctatcgcga agcactgaag caaattggcg aggccgtgta
     5821 tcaggcaatg gaatgaattc gaattagtgg cgaggcccct gatttcctct cacgaatcgc
     5881 ctgtgactcg ttttatcttt gcaggggcat cgccacgctc cagcatggct taggcaaagt
     5941 gtgctttgtt cataccggat gcggcgtgaa cgccttatcc ggcttacaaa tcgcgcaaat
     6001 tcaatagatt gcagaggtaa tgtaggtctg ataagcgtag cacatcaggc agctttgcgt
     6061 ttgtcatcag tctctgatta gtactcgatc catggatcgc gcttgctttc aggcaagggt
     6121 tgctctaatg cctgcatttg ctctgccgaa agtccagtat atcgttgtat ccattcagga
     6181 tccgcaccgt tctgcaaaag caatctaagg aggcgttgtt ccccttcttc tttccccttt
     6241 tccatcccta acagccatcc atcattatga attcgctctg caatcgtcat tattcgctcc
     6301 ctttgttgtg gcattcgacg ggtaagttca ctgataaact cattaaaacg cgcttcatcg
     6361 ccagtcagta aaatgtaatt taacagcgca gttatctggc tgtcattagc acactctgta
     6421 accagtaata ttaccagttg atcgataagc cccatcagat cgcgctggcg aatatgcttt
     6481 tggatcaact ccaacagggc gactctgcga tgctgcacaa tctcgtcgtc tggcacgaca
     6541 gtgacatcca ccagcgggaa cgctgcggta taaagcttcc gtgcggtggt cgggtcagca
     6601 aattcgtcca gccagcacag ggaccaggga taaggactac ggctaccgtg ataaaacagc
     6661 atcgggatga ccagcggtag cggtcggcgt ttatcatgct cgatatggcg ctgcatcacc
     6721 gccatggaat atcgcatcag gcgaaaggcc atatggatat cctcgcggct ctgatgttca
     6781 atcactacgt aaatataacc atcaccttca cgggtcttta ccgaccacag aatatcggag
     6841 tgtagcgccc gcaatttttc atcgacgaag ctggcggatt ccagttttaa gctgtcgaga
     6901 tcgcacagtt cacgtaaatc tttgggtaag tggatctcca taaaatcccg cgcggtgtca
     6961 gggtgcgtga gaaaggattt aaataatgcg tcatgcggcg tactggtcgt gaagtttgtc
     7021 atggcgatcc gtcacctgtt gataagatgg cccgacttta gcgactaaca aaaccattct
     7081 cacccggtaa tttcccattc cccgaggggc tttccaggct atttttattc ttctgcaaac
     7141 gatacatttt tccggaaaca ggctcgcatt ttacatttcc tgtacccggc agatattccc
     7201 ctttccaccg cactcttttg attaaccgca aattttaatt acacttaagg tgtatatttt
     7261 ctatgcaacc catcaattca agaggtgtaa tgtgctgatg actatttgta atcgttatac
     7321 attctgacct ggagtcagaa ggtatttctc tgtctgtgta ttcacaggca gtgtggttga
     7381 ttacatgaaa ttcagtacat ttatccttct cgcctgcctt tcgtcattac cgacggtatt
     7441 gaatttcgtt ttccccattg gggttctccg gacaaggagt tgtttgttat gccacgtttt
     7501 tttgcccgcc atgccgccac gctgtttttc ccgatggcgt tgattttgta tgactttgct
     7561 gcgtatctgt cgacggacct gatccagcca gggatcatta atgtggtccg tgattttaat
     7621 gccgatgtca gtctggcccc tgctgccgtc agtctctatc ttgctggcgg tatggcgtta
     7681 cagtggctgc tggggccgct ttccgacaga attggccgca ggccggtgct gattaccggg
     7741 gcgttaattt ttacccttgc ctgcgccgcg acaatgttca ccacgtcaat gacacaattt
     7801 cttatcgcgc gtgcaattca gggcaccagt atctgtttta ttgccaccgt tggttatgtc
     7861 acggtgcagg aggcgtttgg acagacaaaa gggatcaagt tgatggcgat tatcacctcc
     7921 atcgtactga ttgcgccgat tatcggcccg ctttccggcg cagctctgat gcactttgtg
     7981 cactggaaag tcctttttgc catcattgcg gttatgggtt ttatctcatt tgttggctta
     8041 ctgctggcga tgccagagac ggtgaagcgc ggcgcggttc catttagcgc caaaagcgtc
     8101 ttgcgcgatt ttcgtaatgt cttttgcaat cggctgttcc tctttggcgc agcaaccatc
     8161 tctttaagct atatcccgat gatgagttgg gtggctgtct cgccggtgat cctgattgac
     8221 gcaggcggct taacgacctc gcagttcgcc tgaacacagg ttccggtgtt cggcgcggtg
     8281 attgtcgcga atgccatcgt ggcgcgtttc gttaaagatc cgaccgaacc gcgctttatc
     8341 tggcgcgccg taccccttca actggttggc ctcgcgctgt tgattatcgg caatctgctg
     8401 tcaccgcacg tctggctgtg gtcggtgctg ggcaccagtc tgtatgcttt cgggattggt
     8461 ttgattttcc cgaccttatt ccgctttacg ctgttttcca ataacttacc gaaagggacc
     8521 gtctccgcat cgctgaatat ggtgatcctg atggtgatgt ctgtctcggt cgaaatcggc
     8581 cggtggctgt ggtttaacgg cggtcgcttg ccgtttcatc tgttagccgt cgtggcgggc
     8641 gttatcgtcg ttttcaccct ggcgggattg ctcaatcgcg tgcgccaaca tcaggctgcc
     8701 gagctggcgg aggagcggtg atttttgcgc gatcccgccg tcaggctcta ttcttaacat
     8761 tatgaataaa ctcattgaac tcagacgcgc caaaatgttg gcgctctctt tactgcttat
     8821 cgccgctgct acctttgtcg ttacgctgtt tttgccgccc aatttttggg tgagcggcgt
     8881 gaaggcgatt gctgaagcgg cgatggtcgg cgcgctggcg gactggtttg cggtggtggc
     8941 gctgtttcgc cgcgtgccga ttccgatcat ttctcgtcat acggcgatta tcccgcgtaa
     9001 taaagaccgg attggcgaaa atctcggtca gttcgtgcag gaaaaatttc tcgataccca
     9061 gtcgctggtg gcattgattc gacgtcacga accggcgttg ttgattggca actggtttag
     9121 tcagccggag aacgcccgcc gcgttggtca gcatctgttg cagatcatga gcggttttct
     9181 tgaactgacc gatgatgcgc gtattcagcg cctgcttaag cgcgcggtcc accgggcgat
     9241 tgataaggtt gatctttctg gcaccagtgc gttgatgctg gagagtatga ccaaaaacga
     9301 tcgtcatcag gtgctgctcg atacgctgat cgcacagttg atcgcccttc tccagcgcga
     9361 taaatcgcgc aagtttatcg cccagcaaat tgttcgctgg ctggagagcg agcatccact
     9421 gaaagccaaa attttgccca ctgaatggct gggcgaacat agcgcggagt tggtttctga
     9481 cgcggtgaat tctttgcttg atgatatcag ccgcgatcgt gcgcatcaga tccgccatgc
     9541 gtttgatcgc gccacctttg ccctgatcga caagctgaaa aacgatcctg aaatggcagc
     9601 gcgagccgat gccgtaaaaa gctatctgaa agaagacgaa gcttttaacc gctatctcag
     9661 tgaattgtgg ggggatttac gggaatggct gaaagtggat atcaacagcg aagattctcg
     9721 tgtgaaagaa cgtatcgcac gagcgggtca atggtttggc gaaacgttaa ttgccgatga
     9781 tgccttgcgg gcgtcgttaa atggtcatct cgaacaagcc gcgcaccgcg tcgcgcctga
     9841 gttttccgca ttcctgacgc gccacatcag cgatacggta aaaagctggg atgcgcagga
     9901 tatgtcgcgg caaatcgagt taaatatcgg caaagatctg cagtttatcc gtgtcaacgg
     9961 tacgctggtt ggcggttgta ttggacttat tttgtatttg ctgtcgcagc tcccggcctt
    10021 gttccccctc ggcaatttat agaaatccat gaaaaactaa gtggctaacc gcgagagaga
    10081 tcaaataaac cacactaaaa gaatggtaat aatgtggcct cttttcatta tctcccgtgg
    10141 tacggggaag gaaaatcatg tcacttgtca ccgatctacc cgccattttc gatcagttct
    10201 ctgaagctcg ccagaaaggc tttctcaccg tcatggatct caaggagcgc ggcattccgc
    10261 tggttggcac ctattgcacc tttatgccgc aagagatccc gatggcagcc ggtgcggttg
    10321 tggtttcgct atgttccacc tctgatgaaa ccattgaaga agccgagaaa gatctgccgc
    10381 gcaacctctg cccgctgatt aaaagcagct acggcttcgg caaaaccgat aaatgcccct
    10441 acttctactt ttctgatctg gtggtcggtg aaaccacctg cgacggcaaa aagaaaatgt
    10501 atgaatacat ggcggagttt aagcccgttc atgtgatgca actgccaaac agcgttaagg
    10561 acgatgcctc gcgtgcatta tggaaagccg agatgctgcg cttgcaaaaa acggtagaag
    10621 aacgttttgg gcacgagatt agcgaagatg ctctgcgcga tgccattgcg ctgaaaaacc
    10681 gcgaacgtcg cgcactggcc aacttttatc atttggggca gttaaatcct ccggcgctta
    10741 gcggcagcga cattctgaaa gtagtctacg gcgcaacctt ccggttcgat aaagaggcgt
    10801 tgatcaatga actggatgca atgaccgccc gcgttcgtca gcagtgggaa gaaggccagc
    10861 gactggaccc gcgcccacgt attttaatca ccggctgccc aattggcggc gcagcagaga
    10921 aagtggtgcg cgcgattgaa gagaatggcg gctgggttgt cggttatgaa aactgcaccg
    10981 gggcgaaagc caccgagcaa tgcgtggcgg aaacgggtga tgtgtacgac gcgctggcgg
    11041 ataaatatct ggcgattggc tgctcctgtg tttcgccgaa cgatcagcgc ctgcaaatgc
    11101 tcagccagat ggttgaagaa tatcaggtcg atggcgtagt tgatgtgatt ttgcaggcgt
    11161 gccataccta cgcggtggaa tcgctggcga ttaaacgtca tgtgcgccag cagcacaaca
    11221 ttccttatat cgctattgaa acagactact ccacctcgga tgtcgggcag ctcagcaccc
    11281 gtgtcgcggc ctttattgag atgctgtaag gagtggcagt gacatattcg attggcattg
    11341 attccggctc aaccgccacc aaagggatct tactggctga cggcgtgatt acgcgccgtt
    11401 tcctcgttcc aacatccttt cgcccggcaa cagcaattac tgaagcctgg gaaactctgc
    11461 gcgaagggtt agagacaacg ccgtttctga cgctcaccgg ctacggacgg caactggtgg
    11521 attttgccga taaacaggtg acggaaatct cctgtcacgg gctgggcgca cggtttcttg
    11581 cgccagcaac gcgcgcagta atcgacatcg gtggtcagga cagcaaagtg attcagcttg
    11641 atgatgacgg taacctgtgc gatttcctga tgaatgacaa atgcgcggcg ggcaccgggc
    11701 gtttcctgga ggtgatctcg cgcacgcttg gcaccagcgt cgaacagctc gacagcatta
    11761 ccgaaaatgt cacgccgcac gccatcacga gtatgtgcac agtgtttgct gaatcagaag
    11821 tgatcagcct gcgctctgcg ggcgtagcgc cagaagcgat tctcgccgga gtgattaacg
    11881 cgatggcgcg gcggagtgcc aatttcattg ctcgtctctc ctgtgaagcg ccgattctgt
    11941 ttactggtgg cgttagccac tgtcaaaggt ttacccacat gctggaaagc catctgggaa
    12001 tgccggtaca aactcaccct gatgcgcaat tcgctggtgc aattggcgcg gcggtgatcg
    12061 gtcagcggca gagaaaacgc gcatgaaaga gcatctgttt ttatttcact ccacggtcgg
    12121 cgtcatacaa acccgcaagg cgttacaggc agcgggcatg acttttcgcg tcagcgatat
    12181 tccccgtgat ttacgcggcg gctgcgggtt atgcatttgg ctgacctgtc cgcctggcga
    12241 ggaaattcaa tgggtgatcc ctgggcatac ggaatcagtt tattgtcagc aggatggtgg
    12301 ctggcgctgc attgcacatt acgggatttc tccgcgttaa ggattcatta agatcgctac
    12361 gtatacttct cgatatctta ttgatttgcc ttaaagggat taccgtgaca aaaagtattt
    12421 cattaagtaa acgcatcttc gttattgtga ttctttttgt cattgtggca gtgtgtactt
    12481 tcttcgtgca atcttgcgcc cgtaaaagca atcatgcagc atcatttcag aattaccacg
    12541 ccaccattga cggcaaagaa attgcaggca taacgaataa tatctcttcg ctgacctggt
    12601 cggcacaaag caatactctt tttagcacta tcaacaaacc tgccgccatt gttgaaatga
    12661 ccaccaatgg tgatttgatc cgcacaattc ctctggattt tgttaaggat ctcgagacga
    12721 tcgaatacat tggcgataat cagtttgtca ttagcgatga gcgcgattac gccatttatg
    12781 tgatttcact gaccccaaac tcggaagtaa aaatcctcaa aaaaataaaa atcccgctgc
    12841 aggaatcccc aaccaattgc ggttttgaag gattggccta ttctcgccag gatcacactt
    12901 tctggttttt taaagagaaa aacccgattg aggtctacaa agttaacggg ctattaagta
    12961 gcaatgaatt gcacatcagc aaagacaagg cactacaacg gcaatttact ttagatgatg
    13021 tgtccggcgc agaatttaac caacaaaaaa atacgcttct ggtgctgtct catgaatctc
    13081 gcgcactcca ggaagtaacg ctggttggcg aggttatcgg tgaaatgtca ctaaccaaag
    13141 gcagtcgagg tttgtcgcat aacatcaaac aggccgaagg ggttgcgatg gatgcctctg
    13201 gtaatattta tatcgttagc gaaccaaatc gtttttatcg ctttacccct caatcatcgc
    13261 attaacaggc tggcatgctt cacctttttg ttattaactt gttaacaaca aaaatgacaa
    13321 acaaggttaa acatgccttc gtccacgcat cccgtagaac gcttttcttt cagcaccgcg
    13381 cttttcggga tgctggttct gaccttaggt atgggtttag gccgcttcct ttatacgcct
    13441 atgttgcccg tcatgatggc ggaaggatcg ttttcattta gccagctctc gtggattgcc
    13501 agcggcaact atgcggggta tctggctggc agtctgctat tttcttttgg cgcatttcac
    13561 cagccatcgc gcctgcgccc atttctgttg gcttctgccc tggcgagcgg gttgttgatc
    13621 ctcgcaatgg catggttgcc gccatttatt ctggtgttat tgattcgcgt cctggcgggt
    13681 gtcgccagcg ccggaatgct gatttttggt tcgacgctga ttatgcagca cacgcgccat
    13741 ccttttgtgc tggcagcttt gttttctggc gttggcattg gcatcgcact gggcaatgaa
    13801 tatgttctgg caggcctgca ttttgacctc tcctcgcaaa cgttatggca aggcgccggg
    13861 gcgctttctg gcatgatact gattgcactt acgcttttaa tgccctcgaa aaaacacgcc
    13921 atcgtaccaa tgccattggc aaaaacggag caacagataa tgagctggtg gttactggct
    13981 attctgtacg gcctggcggg atttggttat atcatcgtcg cgacttacct gccgctcatg
    14041 gcgaaagatg caggctcacc attgttaacc gcccatctct ggacgttagt cggcttatca
    14101 attgtgcctg gttgctttgg ctggctatgg gccgcaaaac gttggggggc tttgccctgc
    14161 ctgacggcga atttgctggt gcaggctatc tgtgtgctgc ttactctcgc cagcgactcg
    14221 cctctcttgc ttatcatcag cagtcttggt tttggcggta cctttatggg cacgacatct
    14281 ctggtgatga ctatcgcccg ccaactgagc gtaccgggaa atctcaatct tttaggcttt
    14341 gtgacgctca tttacggcat tgggcaaatc ctcggcccgg cgctgaccag tatgctcggc
    14401 aatggaacat cggcgcttgc cagcgccact ctctgcggcg cggcggcgtt gtttattgct
    14461 gcgcttatca gcacagtgca attatttaag ctacaagtgg tcacttcgtg agagaagttt
    14521 cccttaccac tcaagaaact caaccgcaac tgttgatgtc agccagaccc cgttttccgc
    14581 ttgccaaaaa gggatgcctc gtttcgccat ttcctgtgct ttgacggtta aaataactgg
    14641 cgatccgtgt cttgctccca ctttgcgtgc cgttgcttca tcagcagaga gatgcacgta
    14701 atggcgctcc cctgcaatca gcccttgttt ttttatctcg tcaagaaaac ggcttgccgt
    14761 accatggtag agaaattgtg gcggtgtttt ttcgatgaag ccaatggcga cctgcgaagt
    14821 cgaatgcccc tgaacggcgc gaatacacgt accatcactg gaataactaa agcgcttttt
    14881 atcgctggta gcgacaacga tatccagtaa tgcgcgggtt aaccgcttgc ccgctttttg
    14941 ggcacagagt atgagcttat cgatatccgc ccatccttca cggtccagca cgatgccaat
    15001 cgcctctggt ttatgtcgga ggacaaaact taaaaacttg ctcgtctctg ccagctcttt
    15061 gtcgttgtat tttgccatct cgttctcgtc cctggatttt ctgtttttat ttactgatgt
    15121 cattgtcact gggtttatga attttctgca actcaatcct gcgcagaacc tttgccacac
    15181 agcgtcattt ttccgctatg caaaaaacgc atagccagaa acatacttgc attttcccca
    15241 tacccacatg gctgataagg tttttcagct ccaggaattt tcctggctca agcaacacac
    15301 aaacacaaaa aaacaatggg gattgttatg acacaacaag gggatgctgt agccggggag
    15361 ctggcgacag aaaaagtggg cattaaaggg tatctggctt tttttctcac cataatcttt
    15421 ttttccggtg ttttttccgg gacagacagt tggtggcgcg tttttgattt ttccgtgctg
    15481 aacggatcgt ttgggcaact gcctggtgcg aatggcgcca ccacctcgtt tcgcggcgca
    15541 ggtggcacag gagctaaaga tggctttctc ttcgcgctgg aactggcacc gtcagtcatt
    15601 ctctctctgg ggattatttc aattaccgat ggtcttggcg gattacgcgc cgcgcagcag
    15661 ttaatgacgc ccgtgctaaa accgttgttg ggcattccag gcatctgttc actggcgctg
    15721 attgctaacc tgcaaaacac cgatgccgcc gcaggtatga ccaaagaact ggcgcaggaa
    15781 ggggaaatta ccgaacgtga taaagtgata ttcgccgcct atcaaaccag cggtagcgcc
    15841 atcatcacca actacttctc ttccggcgtc gcggtgtttg cctttctggg cacgtccgta
    15901 atcgtacctt tagccgtcat cctggtgttt aaatttgtcg gagccaacat tttgcgcgtc
    15961 tggcttaact tcgaagaacg tcgcaaccca acgcaaggag cgcaagcatg acaactcagg
    16021 tccgcaaaaa cgtcatggat atgtttattg atggcgcgcg ccgtggcttt actatcgcta
    16081 ccaccaacct gctgccaaac gtggtgatgg cctttgtcat catccaggcg ctgaaaatta
    16141 ctggcctgct cgactgggtg ggacatatct gtgaacccgt aatggcgctg tggggattgc
    16201 ccggtgaagc ggcaaccgtc cttttagccg cattgatgag catgggcggc gcagtcggtg
    16261 ttgccgccag tctggcaacc gctggcgcat taaccggcca cgatgtcacc gttttactgc
    16321 ccgcgatgta tttaatgggc aacccggtgc aaaatgtcgg tcgctgcctg ggaacagccg
    16381 aagtgaatgc caaatattac ccgcatatca ttacggtctg cgtgattaac gcattgctgt
    16441 cgatctgggt tatgcagctt attgtttaac aaggagttac catgattgat tattccgcag
    16501 ccggttttac cctgctgcag ggagcgcatt tgtatgcgcc ggaagatcgg ggaatttgcg
    16561 atgtcctcat cgctaacggc aaaattattg ccgttgccag caacatccct tctgacattg
    16621 taccagactg cacggttgtc gatctcagcg ggcagatcct ctgcccaggt tttattgatc
    16681 aacacgtcca tttgattggt ggtggcggcg aagcaggtcc cacgacacgc acgccggaag
    16741 tggcgttaag tcgtctgacg gaagcgggcg tcacgtcagt ggttggtctg ctgggtaccg
    16801 actctatctc tcgccacccg gaatccctgc tcgccaagac ccgtgcgctc aatgaagaag
    16861 gcatcagcgc ctggatgctg accggcgctt atcatgtccc ttcccgcacc attacaggtt
    16921 ccgtggaaaa agacgtggcg attatcgatc gcgtgattgg tgtgaaatgc gccatctctg
    16981 atcaccgttc tgccgcacct gacgtttatc acctggctaa tatggcggcg gaatcccgcg
    17041 ttggcggttt gcttggcggt aaacctggcg tcaccgtgtt ccacatgggc gacagcaaaa
    17101 aggcgttaca gcctgtctat gacctgctgg aaaactgcga tgtgccgatc agcaagctgc
    17161 tgccgaccca cgttaaccgc aacgttccgt tgtttgagca ggcgctggag ttcgcgcgca
    17221 aaggcggcac catcgacatc accagtagca ttgacgaacc ggtcgcccct gccgaaggta
    17281 ttgcccgcgc cgttcaggcg ggtattccgc tggcacgcgt caccctcagc tccgacggca
    17341 acggtagcca gccgttcttt gatgacgaag gaaatttaac ccatatcggt gttgcaggtt
    17401 ttgaaacatt gctggaaacc gtgcaaatgt tggtcaaaga ctatagcttc agccttagcg
    17461 atgccctgcg cccgctcacc agtagcgtag ccggtttcct taacctgacc gggaaaggcg
    17521 aaattctgcc aggcaatgac gcagacttac tggtcatgac gcctgaattg cgcattgagc
    17581 aggtatacgc tcgcggcaaa ctgatggtca aagacggcaa agcctgcgtg aaaggaacgt
    17641 ttgaaacggc ataaccggtt gtcagccgcc cgaatggacg tagaatgccc gcaagggcgg
    17701 ctgacagagt ataacgtaat ggatgactgt ggtgcggttt tgcataacat tgaaaccaaa
    17761 tggctttatg attttctgac cctggaaaaa tgtcgcaatt tttcccaggc ggcagtcagt
    17821 cgcaacgtct cgcaaccggc attcagccgc cgcatccgtg cactggaaca ggcgattggc
    17881 gttgaattgt ttaaccgcca ggtgacgccg ctgcaactct cggaacaagg gaaaatcttc
    17941 cattcgcaga tccgccatct gttgcaacag ttagaaagca acctggcaga gctgcgtggc
    18001 ggcagcgatt acgcgcaacg taaaatcaag atagccgctg cacactctct ttccctcggt
    18061 ctgttaccgt ccattatcag ccagatgccg ccgctcttta cctgggcaat tgaagccatt
    18121 gatgtcgatg aagcggtcga taaactgcgc gaagggcaaa gtgactgtat tttttccttt
    18181 cacgacgaag atttgctgga agcgccgttt gaccacattc gcttatttga atctcaatta
    18241 ttccccgtct gcgccagtga cgaacacgga gaagcactgt ttaacctcgc gcagccacac
    18301 tttccgttac tgaattacag ccgcaactcc tacatggggc gattgattaa tcgcaccctg
    18361 acgcgccaca gtgagttgag tttcagcacc ttttttgtct cttcgatgag cgagctatta
    18421 aagcaggttg ccctcgacgg ctgtgggatt gcctggctgc cggaatacgc tatccaacaa
    18481 gaaattcgca gcgggcagct cgttgtactg aatcgggaag aactggtgat cccgattcag
    18541 gcctacgcat accggatgaa tacccgcatg aatccaattg ccgaacgctt ctggcgtgaa
    18601 ctgcgcgagc tggagattga acctggttga aagttttctg cagaaaatct tccggcgctg
    18661 ggaattatga ttgaggttcc tgccgcagtc atgattgcag acaaattagc cagtgaagtc
    18721 gatttctttt ccatcggcac caacgatatg acgcaatata ttatggcggc agacagagga
    18781 aatagcaccg tcgcgaaact ggtcgattat tgtaatgacg cggtgattaa cgctattgct
    18841 atggtgtgtc aggccggacg taataatgaa attccggtca gtatgtgcgg cgaaatggct
    18901 ggagatatcc agcaaacagc acgactgctc accatgggga ttgataagct cagtgccagt
    18961 ccatcccggc ttccggcctt gaaagcagca atcagagcct cccactaatt gtgacctaac
    19021 gcgatacgtc ttgcgcgtat cgcgtttccc gtcatttact gcggcatcgt gcgatgccag
    19081 ttccccttct gccagaccag gtcaaacgtt agcggctcat catcgtggta cagcatcatc
    19141 tccagactaa tggccaactc gtggcaatag gcttctttca gattgacctg caatgccgca
    19201 atacgtggct catgaaataa aagcgcggaa cgtaactggc ggcaatacca actctggaga
    19261 agcttgtcgc tctgattatg cccttgccac tcaggcatgc ctgaaacaag taatattggt
    19321 agcggtgaat ggggagaact aaaaagttga aagagatccc ggcatacgga atcacgcagc
    19381 gataacgtat tgtgtcctgt actttcaggt aataccgtct gaactgattg gcgcatcatt
    19441 ttgcgcactc ctgacgttta gtggcatctg tatcctgccc tggctgccgg acaccactgg
    19501 gttgatgctc atattccata atcattcctt tatgctcatt gcgtccctgg cactttcata
    19561 agggatttat aagatccgcc aaagataaaa cactgtacag aaaagaatat ccactgactt
    19621 tttatttaaa aaggtatcat ttgacaaaag tcattaaaca taacggaaat acgttaacat
    19681 attctgatta ttccgaataa tttctcagcg ttaaactgat atataattat tccaaaacaa
    19741 tatatcgtac gtaatgatta atttacgcat aatatattct gcagaacata attaactatt
    19801 cagggaacat acatagtgca aagcggttca actttgcacc agaaatgagg gatcaggcgt
    19861 cgactttatc cagcggccat gaatcaaaca gatagccgtc aaaataaata tcgtcaacgt
    19921 tcgagaattc cagcagacgt tgtttaacgt tttccagatg ctgccacatt gccagcttcg
    19981 ccgcacgggc atcttttttg attaacgcgg cgaggatctg tttgtgatcg cccaaccact
    20041 ctttgcgata caggctgtca tcgagatggc tgtgcaattg gatccacatt ggattgtttt
    20101 cccgccactg ccaggactga cggaataact ccaccagcat gctgttatgc gttgcttcgg
    20161 caatggcgag atggaactgc atatcaccgc tttcgctgct acccggcgcg ctggaagcca
    20221 gttcacgctc ttccagttgc aatgcctgac gcattttgac gatatcttcg cgggtagcct
    20281 gcaaagcggc aaattccgcg atgttacttt ccagtaactg acgcgcctgt aacagctcaa
    20341 aaggaccggc atcattacag acgttggcat ccggactgtc tgtgttgtgg ctgcctgagc
    20401 tatcaagaac ataaataccg gcaccccggc gcacttccac cagccctttg atctccagca
    20461 tgatcagcgc ttcacgtacc accgtccgcg tgacatcaag catttctgca atttcacgct
    20521 ccggtggcag ccgttcgcca ggattgtacg gcgtctttat gatcagatcg cggatcatcg
    20581 ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga tttcatggtg ttgtgtccgt
    20641 taggtgcggt taatcaaatt ttttccggat gcggcgtgaa cgccttatcc gacctacaac
    20701 gggcaatttt acggcacccg ttaagctcaa actataccat tagcactgga tgttggcacg
    20761 tacaggtggg caaaaaaacg aaaactggtc aaccaaatat tagtttgatt gaccagtaag
    20821 tctgtagggt aatcagttag ttacagcgca gccacacact cgcgcgcacc gcgctcacac
    20881 agctgctgat atgccgccgt caccgcgcca acaaagtcgg cgttctgcgg cagatcatcg
    20941 gcaaagatac cgctcaggcc cagcagtgct ttcacgcggt ctgcaccctg atactgcgcg
    21001 ttgatcttct ggaactccgc cagcatcggg tcaaccacgt caatggcatt accctgctca
    21061 tccacgccct gggtgtaacg catccagccc gccacgccca gcgccaggtg acgccagcta
    21121 ccgccgtttt gcaggtgcag acgcaccggg tccagcagac gctgcggtaa cttctggctg
    21181 ccgtccattg caatctgcca ggtacggtga cgcagagacg ggttgctgaa acgctcgatc
    21241 agcagcgtcg cataagcgtt caggtctgta ccttccggca tcgacagcgt tggcgcttgt
    21301 tcctgcatca tcagggcaaa ggccgctttg cgataagccg ggttagtcat ggtgtcggca
    21361 atagtttcat agccgccgag gtaaccgagg tacgccagga aagagtggct accgttcagc
    21421 atacgcagct tcatcatttc gaacggtaca acatccgcaa caaactgcgc gccgacttta
    21481 tcccagtccg gacgaccatt aacgaagtta tcttcaatta cccactgacg gaacggttcg
    21541 caggcaatgg cgcacgggtc gtaaacaccc agctggtcag caatttcctg taaggtttct
    21601 ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg ggaaggtgac gttctcttca
    21661 atccatgccg ccagttgcgg gtcacgcgcc tgagccagcc caagaacggc aaccttcgcc
    21721 acatgaccgt tttcacgcac gttatcgcag gacatcaccg taaatgcttt cagccctttt
    21781 tcacgacgca gacgcagggc ttctacgatg taaccaatcg cagacttcgg cgcagtcggg
    21841 ttttccagat cgtgcttgat cagcgggtta ttgagatcca gttgaccgct tgccgcatct
    21901 gcgcagtagc ctttttccgt gaccgtcaga gagacaatcg ccgtctgcgg acgcgccatc
    21961 gcgttgagaa taccttcgca accgtcgatt tccgggtgca gggcttcttt catcgaaccg
    22021 ataattttca gctcggtgct ttctgcgcct ttttccgcca cggtgtacag cagttgctgt
    22081 tttttcaggt tttcgatcag cacgcggtcg ttgcctggca tcaggttaac ttcgcagatc
    22141 ccccagtcgc tatccgtgct ttccagcagg tgatgagtgt acagcgcctg gtgcgcgcgg
    22201 tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca gacgagaatg atcccatgac
    22261 gggcgggcaa ccggcagatt gctgtcaaca atagtagtca tcgtaaactc cagaatccgt
    22321 cttcagagag tgacggcaag gaagaaggaa ccggcacggc gcgcagccat gccggtggat
    22381 attaacggct aaagaaagcg cgctggatcg ccagttcgac accgcgaact tcggccaggc
    22441 ctttcagacg accaattgcg gagtaaccag gattggtttt cttcttcagg tcgtccagca
    22501 tctgatgacc gtggtccgga cgcatcggga tcaggtcttc tttgccttcc gctttacgac
    22561 ggtgttcttc ttcaacaatc gctttcacca cttcgtacat atcaacgtca ccgttcaggt
    22621 gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat ggtggagcgc agatgggtga
    22681 agtaaatacg cggaccgaac tgcttgatca tatcaaccag atcgttgtca gcacgcacgc
    22741 cgtaggaacc ggtgcacatg gtgaaaccgt tcgccatgct gtttacggta tcaaccatcc
    22801 actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc gaggatcggg cgcggcggat
    22861 cgtccgggtg aaccgccata cgcacgccaa cttcttcagc aactggaata atcgctttca
    22921 ggaagacagc gaagttttcg cgcagcttcg ctttgtcgat atctttgtac agctccaggt
    22981 gcttacggaa ctggtcgagg gtataccctt cttccgcacc tggcagaccg acaatgatgt
    23041 tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc gaagcgaaca gctgcctgag
    23101 caatttcttc ttcggtgtaa tccgcttccg cgcctggacg cttcaggata tgcatttcga
    23161 atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga gccgtctggc agcacgtatt
    23221 cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt gtagcacacg gtgcgaatgc
    23281 cgcactgcgc caggttgcgc agggtctgct gatagttagc aatccactgc tcatagttgc
    23341 cagtgtgggt tttgatatct tcgtgaattg gtacgctttc aacgacagac cacaccaggc
    23401 ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc tacggaccat acttcgccgt
    23461 tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc cgcctgacgg acatcagcta
    23521 aagaaaccgg atcgtttggg ccgtaccagc gccaggtctg ttccatgatt tttcctcttt
    23581 cacatagtgg gttccacagc aaagagacgt ggagccccgg agttgtttag gtatccggat
    23641 gaacaaccag accgcagccg tggcgttgca tcggatgaga tggcgtataa gttctacctt
    23701 ttggtaagac atgtcaaccc gaaatctgaa attggttaac cacatcacaa gaatttcact
    23761 ctcctggttg ttgctgcaaa ttgcagcaaa gggtaatcga tggttattga tcagaggatg
    23821 tgacattcat cgcaacaatg gttgaccaat ttacataaca tgtcgcccaa aataacaacg
    23881 gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac attctctggg tggtattcgg
    23941 cattggtctg atgctggtac tgaatttgaa gttcaaaatc aattcaatgg tggctttgtt
    24001 ggtggcggcg ctgtccgtcg ggatgctggc gggcatggat ttgatgtcgc tgctgcacac
    24061 catgaaagcg ggcttcggca acacgctggg ggaactggct atcatcgtgg tgttcggtgc
    24121 ggtcatcggt aaattgatgg tcgactccgg cgcggctcac cagatagcac atacgctgct
    24181 ggcgcgtctc ggtctgcgct atgtacagct gtcggtgatt atcatcggcc tgattttcgg
    24241 tctggcgatg ttttatgaag tggcctttat catgttagcg ccgctggtta ttgttattgc
    24301 cgccgaagct aaaattccgt tcctgaaact ggcgatcccg gcagtagcag ctgccactac
    24361 cgcacattca ctgttcccac cgcagccggg tccggtggca ctggtgaatg cttatggcgc
    24421 ggatatgggg atggtttata tctatggcgt actggtgacg atcccaagtg tcatctgcgc
    24481 aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc ccaacgccat cattcctgaa
    24541 agcagatcaa ccggtagata tgaataatct gccctctttc ggcgtttcga ttctggtgcc
    24601 gctgatcccg gcgatcatta tgatctctac caccatcgcc aatatctggc tggtaaaaga
    24661 tacccctgcc tgggaagtgg ttaactttat cggttcctcg ccgattgcaa tgtttattgc
    24721 gatggtggtt gcattcgtac tctttggcac cgcgcgtggt catgacatgc agtgggtgat
    24781 gaacgctttt gaaagcgcgg tgaaaagcat tgcaatggtg attctgatca tcggtgcggg
    24841 tggcgtgctg aagcagacca tcatcgacac cggcattggc gacaccatcg gcatgttgat
    24901 gtcccacggc aatatctcgc cctacatcat ggcatggctg atcaccgtac tgattcgtct
    24961 ggcgacgggt cagggtgtcg tttcggcgat gaccgctgcc gggattatca gtgctgcaat
    25021 ccttgatcca gcaaccggtc agctggttgg cgtgaatccg gcgctgctgg tgctggcgac
    25081 ggctgcgggt tccaacaccc tcacgcacat taacgatgca tctttctggt tgttcaaagg
    25141 ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg ggtctgctgg agctggtcaa
    25201 ctccgtggtt gggctgatta ttgtgttgat tattagcatg gtagcgtaaa agaacaccgg
    25261 agcctgccaa tggcaggctc tgttcggatt gtcggtaaag tgcgctttgt ttatgccaga
    25321 tgcgacgctg acgcgtctta tctggcatac aaagggctaa cgtgcaggtt ttagcttcag
    25381 gtaatattgc gtacctgcat tagcaatgcc ctgtgatttc tttattgata aacaaaagta
    25441 acgccaataa tcgattgcac attccctgca gtcacctgcc ctccggtacg tgcgtaattt
    25501 gccgttaatc ccagacttac cgccgaagtc cccactgctc ctaacgatac cgtgttattc
    25561 gctggaataa tcgtaccgtt gcgcgtcaac tgtacgccga cgccctgtgc tggtgaaaac
    25621 gaggcggtat tggtgaaaat cgagttgccc gcatctgcgg ttgtgccgga gaggtaatac
    25681 cccaggtttt ggcttttcgc acaataaacg gtaagaggaa tcggcaccga accgggatag
    25741 tccggcagag taacggtgac atcacgagca gaaacatcac agccgccagt aggcaccacc
    25801 acatcattat tggcgtaaat attccacaca aactggaaat catcgctgtt atagttgttg
    25861 gtctgtcgca aaattagcac ggcaattaat gagccagctt taatcgccac cccgcccgca
    25921 ctgctcacag gcgtcaaata aagcgccacc ggccacggct tatccgttct cgaattataa
    25981 acaacgcgcg gcgtttcgct agtggtcgga aatggatagc tactgccact atattttacg
    26041 gtcccggaaa aattagataa cacgccgcca taagccgagc ctcgttgcag tgtgacatag
    26101 tctgtaatgg tttccggata atcgttatgg caaaagattt gcgtcgaaag atccacgacc
    26161 aggttttgcc ccacattcac ggcaggcgca aggtttacat aaacattagc gctgccaccg
    26221 ccaataggga tagcggtgcc attggcggtt ttacaggcga atgaccaggc atttaccgac
    26281 cagcccatca gcagtacagc aaacaggtaa ataactcgtt tcattacaat catctcttcg
    26341 gcttcagctg taggtatagg tgatgctaat tactgcctga atggttccct gagtggctcc
    26401 gccatttacc gtcaatgctc tgacctgtaa cgggaagtgc gctgattgtg aggaatcatc
    26461 cacctgaact gttttggttg cgccactatt caatgtgttg ccactgtcat cctgtagctc
    26521 taactggatg ttttgcgcgg tcccctggtt tttataatat ccggtactgt cggctgcccc
    26581 gctgaagccg gcggtgaccc tcgacgttcc caccggacaa ttagtcaact caagcgcaac
    26641 atcatgccag gccgatgccg ccccggcaga catcagactg aaagaataaa gatcgccgag
    26701 atctaccgtg gcattggtgg tggaaaccgt gcacggtttg gcgacgacct taccgttcac
    26761 cgtgatggtg acatcggctg cctgtatcgt cgcacttgcg agcgccaaca ttgccgccaa
    26821 taaataccca cgtttgcgcc atttcatgag catctccagt tactgatatt caagagtgaa
    26881 ggtagccgtg gcattgatat gccccgcagt gacaggcacc tgtgtcgcca ttagccgggc
    26941 gtaaaaattc agcgtatttg gtttacccgg cgtcagggtc gtccacgaaa tcgcggacga
    27001 tggggcatta aggggtatct gattttgttg ctcattcaga agctgtattc ccagtcccgc
    27061 agccgctgac accgtatttt caagtgcaag caggttggca ttgtggctat ctgcaacacc
    27121 ggtaaaccca acttttacgg cagaaacggc attaccacag ggtgacagca aaatacgaaa
    27181 tggaacgaca ggagtcgtcg cgccaatgtt gttaaattgc ttcaccgcgt tttccatcag
    27241 atcaacagta aaattggttg attcagcggc cacactacag ccattatctc tgacatagcc
    27301 gcggatagta atcgtgctat ccgcagccaa agcgtgactt accgccagcc acaaaaaagc
    27361 gcaaagaaga taaaaaggtt tgtttctcat cacgcctcct taacgacatt cagctgatag
    27421 ctgggttaat aactgctgct gactctctgg tggcagttga taattggcga cacagtgagc
    27481 attttcctct tctccccatt tcacctgaac ttttcccgct aaaggcattc cgctgaggta
    27541 aacctgacca ttatccgcaa caatgccgct actctggcta ctctctgatg tcaccatcgc
    27601 cccaaacggc agcggcttat tattgtgggt tagcgtcatg agcagtttta tcccaacgcg
    27661 cgctttaaac tctgctcgca cgatcgcccc acgggtggga acaacgttag cgaccgcgtt
    27721 atctaaatcg acgttatcag ccagggtatt ggtatccagc gctactctgt tttcccgata
    27781 ttcagtggca taaggcagca cggcataacc gcgccagtcg gtacgcaccc ccgtctggtt
    27841 ttcgactttt gcatcttttg cgccaggcgc tttaacaagc accaccgtat cgtttaacgg
    27901 ctgccccagc gttacgccat tggcatgagc cagtacccca ccgctgactc cgtaatagag
    27961 ctgcttaata tcatcgctat ggctgtaacc gatgttggca ttgccgtaac caccgcgata
    28021 attcagcgtg gcgtagcctg tacttccgct attaccatca cctcccccgg cgtaaccggt
    28081 ttgcacgcta tagctgaggt tgttgtcttc cagcaacgta ccgtatacac cggccagatt
    28141 ggtcatccga ccgttgagat cgtgtgacat gctgtaactg gcactggcat gtcgccactg
    28201 agatttactg tcagaacgca gccagtggct gaaaggaata ttgacgttaa gcgctaacat
    28261 ctgatcccgt cctttttgcc aggcgttttt cgtcaggcta tagctgagcg tccagttgat
    28321 atcttcgaat gcagtattta aaccagcctg gaattgctca tcgacattac tcgttcccca
    28381 ataagtttga tggctaccac tcaaatacag tgttgatgag cgcccgagtt gctgagtaac
    28441 ggtgagctgt aatttcccgc gtttgttata agcgagattg taatagtcgg tgaatttcgg
    28501 cttaacctga ataactccat cctgtgtttc gatgttgtag ccattcattc gactgtatgt
    28561 tgtatcagcg aaattaaaat acccactggt cgaataacgg taacccacta actgaatatt
    28621 cgtgcctgac tcattgagcg atttgttata aagaaaacgc accgattgtc cgtcatgctg
    28681 actgtcatcg ggaagtgtgg aattagcctg cgtcatatcc acagacagag cgcccagtgc
    28741 ccccatattt ttcccgatac cgaaattaaa cgcacgataa cgatccgcca gttgcgttcc
    28801 accatatatt gtccagccag ccggaaggcc gtggagtaat gtactctgga aaaagcgggg
    28861 tttttcctgt tgcgcatttc cactacggta ttctcctgcc gtaatggaat aacgtgtatg
    28921 cccttcacgc tgcaaaagcg ggactgacga atagggtacg gtaaaaatct gcgtactgcc
    28981 gtcagcctct ttaatcgtta cctgcaagtc accactatta cctgcggcat agatatcgtt
    29041 gatggtaaaa ggccccggtg gcaccgtact attataaatg tcatacccat tttgtttaat
    29101 agtgacctgt gcagtaccac gagcaatacc gtggatcacc ggggcaaatc ctctttggct
    29161 atcgggtaac atattgtcat ctgaggccaa ttgtgcgccg cgaaagttaa taccatcgaa
    29221 aatatcgccc tgagtataac catcacccag cgtcagccgg gaacgtaacg gaattatgtc
    29281 tcgctcaagc caggtattga tatgctgcca tttatttttg ctacctgatg aactgtcgct
    29341 actgttataa ctccaggtgg tattgtcgcg taaacgccac gcaccaatat ttaacccact
    29401 ctgtaggttt aaatatgcat aatggctgtt acccccaatt cgattctgta cactatttcc
    29461 gctgaaatta taattgagca atccggcatt aataccggga tcccataact caggaggaat
    29521 ataaccacgc gcgcgattac tcataaatgc ctgagggatc gtcaggttga gtcgctgctg
    29581 accaacatct aaatgcgcag tagcgtcatg gatcattgag gttaatggca cgcaggcatc
    29641 atccgccagc agattcatac cggagacaga agccgtattc agccccatac tggcaagttg
    29701 cgcgcgtgtc aggcagggaa caatcccttg ttcactgtcg cccgtgttaa atgtgacatc
    29761 acgcgttgcc atataaccat tattcaaata gatatcgacg cgatacgtcc ctggcggtaa
    29821 ttcttgccca ttttcaaaac gcgataaatc ggccacagcc tggggatcat ccgctaaaaa
    29881 gcgcggatta aaatagagtt cggcagatga caaaggtgcc tgtgcggcaa aagcacaggc
    29941 gacaaagagc cggacaaaaa aaccagccaa acgatgctta cgaatatgca agcattgtgt
    30001 gtttcgctgg taaagtctta aattcagata tgacattatt cctccggttt aaaatcaccc
    30061 ggcagtcaat tctttttatt caggcgaaaa attccccctg cgttattcca ttacgcccgt
    30121 cattttgggg gtaagtgcgc cataatcatt tattgttcgg taagtaatat tgcttcctgc
    30181 atcagaaggc aatttaaccg tgctttcgcc cattggaggc accaatgcat tttcaagaac
    30241 ccgggttccg gcattcaact ctgttaccgt caggtaatag ggtgtcgggt taatcagcgt
    30301 cagagaattc gcgctacgac gaaatcttaa tttttctgcg gcctgatcgg gtggcaacgc
    30361 taatttagcc gggcgatagt acagcttaat gcggctgata attgcgagct gtagcgtatt
    30421 ctcagtcaat tttgatttat ccattgacgg aatcgcttta acgttcatcc agaataaact
    30481 ttcccgatcc tgtggcaatt ggttatttgt tgcatcaaga atacgtaagg tattctctct
    30541 ttttcccttc atcgcaaaca gaggtggcgt cacgataaaa cgaccatcct ttacaccatc
    30601 ggcattttcc acccatgatt gaattaaata ggtgctattt tcatcattat ttgtcacggc
    30661 aagttgcact tgtttttgcc ctgccggata aattacgcgg gtcgcaccta aggccactcc
    30721 cgcttcagcg cgtccggcaa ccatcattgc catgaacatc aggatacctg ccagcaagca
    30781 gaatgttatt tcctgcgatt tccttacatt gacgttttta ttactcactg tcctgttcct
    30841 gttatcacat tatctgctga acaattactg ataggttaaa gagaaccagg cctgggcatt
    30901 ggcgatgccg ccagtaaccc gacgcccggt agcacgatat ttggcgatga aatgtagcga
    30961 agtagagcct gagtaaagcc gtttccagtt tgctggagga cgattaatcg gtacgaggtt
    31021 tccttcatca tcaaataacg ctacgccaat attggtggct atccctggcc cctctcccac
    31081 ggaaagcaca tccggatttt taccatccgc gacaccgtga aacgccacac ccacacgttc
    31141 actcaccacc gtgctacatt cccgtaaatg aataacaaaa ggcaccggtg cgctatcttc
    31201 ccctaccgca tgaaaccggt tactgctgat tttccccata ttgaccgtca tttgtttatc
    31261 accagcttca actcggcaag tttccgcaat aatgacgccc tgaaattgca tatttccgcc
    31321 gggcagcgtg gtattccatt tatttccggc cagagcaaac attggcagca acgacgctaa
    31381 tagaaataat ctcttccttt tcatcgttat ttttatcgca caagggtggg catccctgcc
    31441 cgtaatgacg tccctgaacc tgggtaggtt attgatactg aaccttgaag gtcgcatccg
    31501 cattagcagc acccggggtt gcctcgccga ttgcataata acgcgcctgg aacggaatgg
    31561 tgttggtacc gttattcaag gttgtttgtg cactaaatgt cgcaccgtcc agcgccagcg
    31621 cagcacccgt tctgtccagg atctgcacac caacttttgt tgcgctaccc gcagctgaac
    31681 tctgcaaagc taatacgtta ggatgagttc catcaattgc ggtaccaagg aaggcaatag
    31741 cagctttgtc tgcaacagta gtgtcacaat cgttcagttg aatgttaaaa ccgacagcag
    31801 aactggttgc tccttcttgt gccagcgatg ctgtacgaac ctgccctaac tgaacagttt
    31861 gatcaacaga gcctgcatca actgcgcaag cggcgttaac aacctcccct ttaaagtgaa
    31921 cggtcccacc atttaccgtc gtgggtgtag tttcagccag agccgctgta gaactcaggg
    31981 acagagccga cagaacaaca attgccagag ttttaatttt catactgctt tcctttcaaa
    32041 aaactatttc taaatcgaca tgggcagtcg ttctgtacac tttgttttgt caacgagttt
    32101 gactgccaac actgcacagt tttcccccaa aagatgaaac atttggggcc attttgactc
    32161 atagaggaaa gcatcgcgca caaacttttt cagttta
//
LOCUS       DAZGHF010000046        31147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGHF010000046 DAZGHF010000000
VERSION     DAZGHF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.046"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(40..2715)
                     /gene="adhE"
                     /locus_tag="ACHK3A_002224"
                     /gene_synonym="adhC"
     CDS             complement(40..2715)
                     /gene="adhE"
                     /locus_tag="ACHK3A_002224"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO8740293.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVAYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3108..3161
                     /gene="ychE"
                     /locus_tag="ACHK3A_002225"
     CDS             3108..3161
                     /gene="ychE"
                     /locus_tag="ACHK3A_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO8740294.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            3192..3839
                     /gene="ychE"
                     /locus_tag="ACHK3A_002226"
     CDS             3192..3839
                     /gene="ychE"
                     /locus_tag="ACHK3A_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO8740295.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(3997..4293)
                     /locus_tag="ACHK3A_002227"
     CDS             complement(3997..4293)
                     /locus_tag="ACHK3A_002227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740296.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSLFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            4577..6208
                     /gene="oppA"
                     /locus_tag="ACHK3A_002228"
     CDS             4577..6208
                     /gene="oppA"
                     /locus_tag="ACHK3A_002228"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO8740297.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIITNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            6294..7214
                     /gene="oppB"
                     /locus_tag="ACHK3A_002229"
     CDS             6294..7214
                     /gene="oppB"
                     /locus_tag="ACHK3A_002229"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO8740298.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            7229..8137
                     /gene="oppC"
                     /locus_tag="ACHK3A_002230"
     CDS             7229..8137
                     /gene="oppC"
                     /locus_tag="ACHK3A_002230"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO8740299.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            8149..9162
                     /gene="oppD"
                     /locus_tag="ACHK3A_002231"
     CDS             8149..9162
                     /gene="oppD"
                     /locus_tag="ACHK3A_002231"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO8740300.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNRLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            9159..10163
                     /gene="oppF"
                     /locus_tag="ACHK3A_002232"
     CDS             9159..10163
                     /gene="oppF"
                     /locus_tag="ACHK3A_002232"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO8740301.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10216..10545)
                     /gene="yciU"
                     /locus_tag="ACHK3A_002233"
     CDS             complement(10216..10545)
                     /gene="yciU"
                     /locus_tag="ACHK3A_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO8740302.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10580..12040)
                     /gene="cls"
                     /locus_tag="ACHK3A_002234"
     CDS             complement(10580..12040)
                     /gene="cls"
                     /locus_tag="ACHK3A_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO8740303.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            12183..12356
                     /gene="yciY"
                     /locus_tag="ACHK3A_002235"
     CDS             12183..12356
                     /gene="yciY"
                     /locus_tag="ACHK3A_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO8740304.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(12411..13664)
                     /gene="kch"
                     /locus_tag="ACHK3A_002236"
     CDS             complement(12411..13664)
                     /gene="kch"
                     /locus_tag="ACHK3A_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO8740305.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(13965..14261)
                     /gene="yciI"
                     /locus_tag="ACHK3A_002237"
     CDS             complement(13965..14261)
                     /gene="yciI"
                     /locus_tag="ACHK3A_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO8740306.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            14485..15204
                     /gene="tonB"
                     /locus_tag="ACHK3A_002238"
     CDS             14485..15204
                     /gene="tonB"
                     /locus_tag="ACHK3A_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO8740307.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(15244..15642)
                     /gene="yciA"
                     /locus_tag="ACHK3A_002239"
     CDS             complement(15244..15642)
                     /gene="yciA"
                     /locus_tag="ACHK3A_002239"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO8740308.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(15747..16286)
                     /gene="yciB"
                     /locus_tag="ACHK3A_002240"
     CDS             complement(15747..16286)
                     /gene="yciB"
                     /locus_tag="ACHK3A_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO8740309.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(16316..17059)
                     /gene="yciC"
                     /locus_tag="ACHK3A_002241"
     CDS             complement(16316..17059)
                     /gene="yciC"
                     /locus_tag="ACHK3A_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO8740310.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(17087..17176)
                     /locus_tag="ACHK3A_002242"
                     /pseudo
     CDS             complement(17087..17176)
                     /locus_tag="ACHK3A_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            17416..18054
                     /gene="ompW"
                     /locus_tag="ACHK3A_002243"
     CDS             17416..18054
                     /gene="ompW"
                     /locus_tag="ACHK3A_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO8740311.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(18113..18619)
                     /gene="yciE"
                     /locus_tag="ACHK3A_002244"
     CDS             complement(18113..18619)
                     /gene="yciE"
                     /locus_tag="ACHK3A_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO8740312.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNDEKHMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(18665..19165)
                     /gene="yciF"
                     /locus_tag="ACHK3A_002245"
     CDS             complement(18665..19165)
                     /gene="yciF"
                     /locus_tag="ACHK3A_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO8740313.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETYGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(19251..19430)
                     /gene="yciG"
                     /locus_tag="ACHK3A_002246"
     CDS             complement(19251..19430)
                     /gene="yciG"
                     /locus_tag="ACHK3A_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO8740314.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(19811..20617)
                     /gene="trpA"
                     /locus_tag="ACHK3A_002247"
     CDS             complement(19811..20617)
                     /gene="trpA"
                     /locus_tag="ACHK3A_002247"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO8740315.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNLAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(20617..21810)
                     /gene="trpB"
                     /locus_tag="ACHK3A_002248"
     CDS             complement(20617..21810)
                     /gene="trpB"
                     /locus_tag="ACHK3A_002248"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO8740316.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(21822..23183)
                     /gene="trpCF"
                     /locus_tag="ACHK3A_002249"
     CDS             complement(21822..23183)
                     /gene="trpCF"
                     /locus_tag="ACHK3A_002249"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO8740317.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPAHIATVYRNYASAISVLTDEKYFQGSFDFLS
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRHLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(23184..24779)
                     /gene="trpD"
                     /locus_tag="ACHK3A_002250"
     CDS             complement(23184..24779)
                     /gene="trpD"
                     /locus_tag="ACHK3A_002250"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO8740318.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHVPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDSQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(24779..26341)
                     /gene="trpE"
                     /locus_tag="ACHK3A_002251"
     CDS             complement(24779..26341)
                     /gene="trpE"
                     /locus_tag="ACHK3A_002251"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO8740319.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQSLLNVPNEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(26363..26456)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(26433..26477)
                     /gene="trpL"
                     /locus_tag="ACHK3A_002252"
     CDS             complement(26433..26477)
                     /gene="trpL"
                     /locus_tag="ACHK3A_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO8740320.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            26615..27496
                     /gene="rnm"
                     /locus_tag="ACHK3A_002253"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             26615..27496
                     /gene="rnm"
                     /locus_tag="ACHK3A_002253"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO8740321.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HYGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            27493..28113
                     /gene="yciO"
                     /locus_tag="ACHK3A_002254"
     CDS             27493..28113
                     /gene="yciO"
                     /locus_tag="ACHK3A_002254"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO8740322.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            28141..30030
                     /gene="yciQ"
                     /locus_tag="ACHK3A_002255"
     CDS             28141..30030
                     /gene="yciQ"
                     /locus_tag="ACHK3A_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO8740323.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYPKNTEIPFYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVIRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAILREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKEKIHLEDHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWEGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEIARNGVLFSLQTHLSCGAYTSLLGRSYSGGGSGAGGG
                     GGGGW"
     gene            30243..31118
                     /gene="rluB"
                     /locus_tag="ACHK3A_002256"
     CDS             30243..31118
                     /gene="rluB"
                     /locus_tag="ACHK3A_002256"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO8740324.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHC
                     QVSGGRRSGGRNNNG"
BASE COUNT         7862 a         7492 c         7835 g         7958 t
ORIGIN      
        1 ccagaagggg ccgtttatgt tgccagacag cgctactgat taagcggatt ttttcgcttt
       61 tttctcagct ttagccggag cggcttcttt cttcgctgca gtttcgcctt ctacataatc
      121 acgaccgtag taagtatcca gcagaatctg tttcagctcg gagatcagcg ggtaacgcgg
      181 gttagcgccg gtgcactggt catcgaatgc atcttcagac agtttatcca cgttcgccag
      241 gaagtctgct tcctgaacgc cagcttcacg gatagatttc ggaataccca gttcagcttt
      301 cagcgtttcc agccatgcca gcagtttctc gatcttagca gcagtacggt cgcccggtgc
      361 gctcagaccc aggtggtcag caatttcagc ataacgacgg cgagcctgcg gacggtcata
      421 ctggctgaat gcagtctgct tggtcgggtt gtcattcgcg ttgtagcgaa taacgttaca
      481 aatcagcagg gcgtttgcca gaccgtgcgg aatatggaac tgggaaccca gtttgtgcgc
      541 cattgagtga catacaccca ggaaggcgtt cgcaaacgcg atacccgcga tagttgctgc
      601 actgtgaaca cgttcacgcg ctaccggatt tttagatcct tcgtggtagg acgctggcag
      661 atattctttc agcagtttca gtgcctgcag tgcctgacca tcagagaact cagatgccag
      721 tacagaaaca taagcttcca tggcgtgagt tactgcgtcc aggccaccga aagcacacag
      781 ggacttcggc atgtccataa ccaggttggc atcgacaatc gccatatccg gggtcagcgc
      841 atagtctgcc agcggatatt tctgaccagt agcgtcgtca gttacaaccg caaacggagt
      901 gacttcagaa cctgtaccag aagtggtggt gacagcgatc attttcgctt tcacgcccat
      961 tttcgggaac ttgtagatac gtttacggat atccataaag cgcagcgcca gttcttcgaa
     1021 gtgagtttct ggatgctcgt acatcaccca catgatcttc gcggcatcca tcggggaacc
     1081 accacccagc gcgataatca cgtctggttt gaaggagttt gccagttctg cacctttacg
     1141 aacgatgctc agggtcgggt cagcttctac ttcgaagaag acttcagttt caacgcctgc
     1201 tgctttcagt acggaagtga tctgatcagc ataaccattg ttgaacagga agcggtcagt
     1261 cacgatgagc gcacgtttgt ggccatcagt aatcacttca tccagcgcga ttggcagaga
     1321 gccacggcgg aagtagatag atttcggaag tttgtgccac aacatgtttt cagctcgctt
     1381 agcaacggtt ttcttgttga tcaggtgttt cggaccaacg ttttcagaga tggagttacc
     1441 accccaggaa ccacaaccca gagtcaggga aggtgcgagt ttgaagttat acaggtcacc
     1501 gataccaccc tgagacgccg gggtgttgat cagaatacgg gccgttttca ttttctgacc
     1561 gaagtaggca acgcgagccg gttggttatc ctggtcagtg tacaggcaag aggtatgacc
     1621 gatgccgccc atagcaacca gtttctctgc tttttctacc gcgtcttcga aatctttagc
     1681 acggtacatt gccagagtcg gggacagttt ttcatgtgcg aacggttcgc tttcatcaac
     1741 aacggtcact tcaccgatca gaatcttggt attttctggt acagagaagc ctgccagttc
     1801 agcaatttta taggctggct gaccaacgat agccgcgttc agcgcaccgt ttttcaggat
     1861 aacatcctga acagctttca gctctttacc ctgcaacaga tagccgccgt gggttgcgaa
     1921 acgttcacgt actgcgtcat aaacagagtc aacaacaaca acagactgtt cagaagcaca
     1981 gattacgccg ttgtcgaagg ttttggacat cagcacagat gcaactgcac gtttgatatc
     2041 agcagtttca tcgataacaa ctggcgtgtt acccgcgcct acaccaatag ctggtttacc
     2101 ggagctgtat gcggctttaa ccatgcccgg accaccagtc gcgaggatca ggttgatgtc
     2161 tgggtggtgc atcagtgcgt tagacagttc aacagaaggt tgatcgatcc agccgatcag
     2221 atctttcgga gcaccggcag cgatagcagc ctgcagaacg atatcagccg ctttgttggt
     2281 tgcatctttt gcacgcgggt gcggggagaa gataatggcg ttacgggtct tcaggctgat
     2341 cagcgatttg aagatagcag ttgaagtcgg gttagtggtc ggaacgatac cgcaaataat
     2401 accgattggt tcagcgatag tgatggtacc aaaagtgtcg tcttcagaca gaacaccaca
     2461 ggttttttca tctttatagg cgttatagat atattcagaa gcaaagtggt ttttgatcac
     2521 tttatcttcg acgataccca tgccggattc ggcaacggcc attttcgcga gtgggattcg
     2581 agcatctgca gcagccagag cggcggcgcg gaagattttg tctacttgct cttgagtgaa
     2641 actggcatat tcacgctggg ctttttttac acgctctacg agtgcgttaa gttcagcgac
     2701 attagtaaca gccataatgc tctcctgata atgttaaact tttttagtaa atcatctgct
     2761 cgaatacgag agtatagtca gtgcggtgat gatttgctta accgatgaaa atcaaaagct
     2821 tactcgcgct cacactcact gtgatttact aaaagagttt aaacattaga gttattatct
     2881 ctaatgcgtc acttccaggt ggcgtaagca agattactca cttctgggta ctgattacgt
     2941 gatccaaatc aaatttttgc aaagctgaca cctttcagca tcgcttttcg ccattatagc
     3001 taacagttaa taaattgtag tatgatttgt tggctacatt agcatgtttt gcacaaccag
     3061 ataacaataa cgaatgatag caattttaag tagttaggag gtgaaaaatg ctgtcaaaag
     3121 gcgtattgtc agcgcgtctt tttaacctta tttatggcta acattatccg gcttttgctt
     3181 cggagctaac cgtgattcag accctttttg attttcccgt ttacttcaaa tttttcatcg
     3241 ggttatttgc gctggtcaac ccggtaggga ttattcccgt ctttatcagc atgaccagtt
     3301 atcagacagc ggcagcgcga aacaaaacga accttacagc caacctgtct gtggccatta
     3361 tcttgtggat ctcgcttttt ctcggcgaca cgattctaca actttttggt atatcaattg
     3421 attcattccg tatcgccggt ggcatcctgg tggtgacgat agcgatgtcg atgatcagcg
     3481 gcaagcttgg cgaggataaa cagaacaagc aagaaaaatc agaaaccgcg gtgcgtgaaa
     3541 gcattggtgt ggtgccattg gcgttgccgt tgatggcggg gccaggggcg atcagttcta
     3601 ccatcgtctg ggggacgcgt tatcacagca ttagctatct gtttggtttc tttgtggcta
     3661 ttgcattgtt cgctttatgt tgctggggat tgttccgcat ggcaccgtgg ctggtacggg
     3721 ttttacgcca gaccggcatc aacgtgatta cgcgtattat ggggctattg ctgatggcat
     3781 tggggattga atttatcgtt actggtatta aggggatttt ccccggcctg cttaattaat
     3841 tcctttcaaa tgaaacggag ctgtcatgct ccgtttactt cgtcattatt tttactttgt
     3901 tcccgcgcag ttatcaaaag caaaaggaat gggtgaaaat attcttctca aattacagtt
     3961 agttataagg atttccttaa ctgcttctcc tcaccatcat gttattttcg ccacatcata
     4021 atcctgggct tgctgaagaa taattgaaat gatattagta attccactgc ctttggtaga
     4081 ggaaagtgct aaataataat caattgttaa attattgtgc atttcactac tggaactgta
     4141 atcagaaaag agagacatgc ttaaccaatc tctatttgat tgaattgaaa gatgtttgtt
     4201 aagacatgga tgcaagctat agattctgat gcggtcaata aaagagaatt gcttaacaat
     4261 tttgcaaaat gtattggcga gtaagaagcg catttggtac tttccgggca accgccaggc
     4321 gattctttat tggtaatgag aataattaac aattaaagag cgtcgcgaaa gaataatgtg
     4381 tctcgacagg ggagacacag tacgaatcga cataaggtga tcgtctgaat caccagaata
     4441 aataaagtcg gtgatagcaa tccgtaacga taaagtaacc tgacagcaga aagtctccga
     4501 gcctgtgcag ggtcccaatc cgggattaca catgctggtt aataccagta attataatga
     4561 gggagtccaa aaaacaatga ccaacatcac caagagaagt ttagtagcag ctggcgttct
     4621 ggctgcacta atggcaggga atgtcgcgct ggcagctgat gtacccgcag gcgtcacact
     4681 ggcggaaaaa caaacgctgg tacgtaacaa tggttcagaa gttcagtcat tagatccgca
     4741 taaaattgaa ggtgttccgg agtctaatat cagccgagac ctgtttgaag gcttactggt
     4801 cagcgatctt gacggtcatc cggcacctgg cgtcgctgaa tcctgggata ataaagacgc
     4861 gaaagtctgg accttccatt tgcgtaaaga tgcgaaatgg tctgatggca cgccagtcac
     4921 agcacaagac tttgtgtata gctggcaacg ttctgttgat ccaaacaccg cttctccgta
     4981 tgccagttat ctgcaatatg ggcatatcgc cggtattgat gaaattcttg aagggaaaaa
     5041 accgattacc gatctcggcg tgaaagcaat tgatgatcac acattagaag tcaccttaag
     5101 tgaacctgtt ccgtacttct ataaattact tgttcaccca tcaacttcac cggtgccaaa
     5161 agccgctatc gagaaattcg gcgaaaaatg gacccagcct ggtaatatca tcaccaacgg
     5221 tgcctatacc ttaaaagatt gggtcgtaaa cgaacgaatc gttcttgaac gcagcccaac
     5281 ctactggaac aacgcgaaaa ccgttattaa ccaggtaacc tatttgccta ttgcttctga
     5341 agttaccgat gtcaaccgct accgtagtgg tgaaatcgac atgacgtata acaacatgcc
     5401 gatcgaattg ttccagaagc tgaaaaaaga gatcccggac gaagttcacg ttgatccata
     5461 cctgtgcact tattattacg aaattaacaa ccagaaaccg ccattcaacg atgtacgtgt
     5521 gcgtaccgca ctgaaactgg gtatggaccg cgatatcatt gttaataaag tgaaagcgca
     5581 gggcgacatg cccgcctatg gttacactcc accgtatact gatggcgcaa aattgactca
     5641 gccggaatgg tttggctgga gccaggaaaa acgtaacgaa gaagcgaaaa aactgctggc
     5701 tgaagcgggt tataccgcag acaaaccgtt gaccatcaac ctgttgtata acacctccga
     5761 tctgcataaa aagctggcga ttgctgcctc ttcattgtgg aagaaaaaca ttggtgtaaa
     5821 cgtcaaactg gttaaccagg agtggaaaac gttcctcgac acccgtcacc agggcacttt
     5881 tgatgtggcc cgtgcaggct ggtgtgcaga ctacaacgaa ccaacttcct tcctgaacac
     5941 catgctgtcg aacagctcaa tgaataccgc gcattataag agcccggcct ttgacagcat
     6001 tatggcggaa acgctgaaag cgactgacga ggcgcagcgc acagctctgt acactaaagc
     6061 agaacaacag ctggataagg attcggccat tgttcctgtt tattactacg tgaatgcgcg
     6121 tctggtgaaa ccgtgggttg gtggctatac cggcaaagat ccgctggata atacctacac
     6181 ccggaatatg tacattgtga agcactaatg gcaatacgtg gggcaggcgt gtactgctcc
     6241 acggtgtctg atttttatcg cattacagaa ggcacaggcc agaaggtagg gcaatgttaa
     6301 aatttattct acgtcgctgt ctggaagcga ttccgacgct atttattctt attactattt
     6361 cgttcttcat gatgcgcctc gcgccgggaa gcccttttac cggcgaacgt actttaccgc
     6421 cagaagtgat ggccaatatc gaagcgaaat atcatcttaa tgatccgatc atgacacagt
     6481 atttcagcta cctgaaacaa ctggcgcatg gcgatttcgg tccatcgttt aaatataaag
     6541 attattcggt caacgacctg gttgcatcca gttttcccgt ttctgccaaa ctgggagccg
     6601 cagcattttt ccttgcggta atactgggtg ttagtgctgg cgttattgcc gcattaaaac
     6661 aaaacaccaa atgggactat accgtgatgg ggctggcaat gaccggggtt gttatcccca
     6721 gttttgtggt tgcgccatta ttagtcatga tatttgcgat cattttgcat tggctgccgg
     6781 gcggtggctg gaatggtggt gcgcttaaat tcatgatact gccaatggtg gcgttgtcac
     6841 tcgcctatat cgccagtatt gcgcgtatta cccgtggctc tatgattgaa gtattacact
     6901 ccaactttat tcgtactgcc cgggcgaaag ggttacctat gcggcggatc attttacgcc
     6961 acgcattaaa acctgctctg ttacccgtgc tctcctatat gggccccgca tttgtcggca
     7021 ttattaccgg ttctatggtc atcgaaacca tttatggttt gccggggatt gggcaactgt
     7081 tcgttaatgg cgcattgaac cgtgactatt ccttagtgtt aagcctgact atcctggttg
     7141 gtgcgttaac cattttgttt aatgccattg tcgatgtgct atatgcggtt atcgacccga
     7201 aaatccgtta ctgatactgg agctcgcgat gatgttaagt aagaaaaaca gcgagacgct
     7261 ggaaaatttc agtgaaaagc tggaggtcga agggcgcagc ttgtggcagg acgcacgtcg
     7321 acgttttatg cataaccgtg cggcggttgc cagtctgata gtgctggtgc taatcgcgtt
     7381 atttgtaatc ctggcaccga tgctttcgca gtttgcctat gacgatactg actgggcgat
     7441 gatgtccagc gccccggata tggagtccgg tcactacttt ggtactgact catccggtcg
     7501 cgacctgctt gtgcgtgttg cgattggcgg gcgtatctca ctcatggtcg gtgttgctgc
     7561 ggcactggtg gcagtggtcg tggggacact ttatggttcg ctttccggtt atctgggcgg
     7621 taaagtggat tcggtaatga tgcgtctgct ggaaatcctc aactccttcc cattcatgtt
     7681 cttcgtcatt ttgctggtga cctttttcgg tcaaaacatc ctgctgattt tcgtggcgat
     7741 tggcatggtt tcctggctgg atatggctcg tattgtgcgt gggcaaaccc tgagtctgaa
     7801 gcgcaaagag tttattgagg cggcacaagt tggcggtgta tcgacgtcgg gcattgttat
     7861 tcgccacatt gtgccgaatg tactcggtgt ggtggtggtc tacgcatcac ttctggtgcc
     7921 cagcatgatc ctctttgaat ctttccttag cttcctgggg ttggggacgc aagagccgtt
     7981 aagcagctgg ggcgcattgc tgagtgatgg cgcgaactcg atggaagtct ctccatggtt
     8041 actgttgttc ccagcgggat tcctcgtggt gacgctattt tgtttcaact ttataggcga
     8101 tggcttgcgt gatgccctcg acccgaaaga tcgttaagga gtgcagccat gagcgtaatt
     8161 gaaactgcaa ctgtgccgct cgcacaacaa caggctgacg cactgctgaa cgtgaaagat
     8221 ttgcgtgtca cctttagtac cccggacggc gacgtcacgg cggtcaatga tttgaatttt
     8281 tccctacgtg ccggagaaac gctgggcatt gtaggtgagt ctggttcggg taaatcgcaa
     8341 actgcatttg cgttgatggg cctgctggct gccaacggac gtattggcgg atcggcaacc
     8401 ttcaatgggc gtgaaatcct caatttgcca gagcgtgaac tcaatagact gcgcgctgaa
     8461 caaatctcaa tgatttttca ggacccaatg acttcgttga atccctatat gcgcgtcggt
     8521 gagcagttga tggaagtgct gatgctgcat aagaacatga gcaaagctga ggcgtttgaa
     8581 gagtcggtgc ggatgctcga tgcggtaaaa atgccggaag cacgtaaacg catgaaaatg
     8641 tacccgcatg aattttctgg tggcatgcgt cagcgagtca tgattgcgat ggcattgcta
     8701 tgtcgaccta agctgctgat tgcggatgaa cccactactg cgctggacgt caccgtacag
     8761 gcgcagatca tgacgctatt aaatgaactg aagcgggaat ttaataccgc catcattatg
     8821 atcacccacg atcttggcgt ggtggcgggg atctgcgaca aagtgctggt aatgtacgcc
     8881 gggcgcacga tggaatatgg caacgcgcgc gatgtctttt atcaacccgt tcatccttat
     8941 tctatcggtt tgctcaacgc ggtgccgcgt ctcgatgcgg aaggtgaaac aatgttgacc
     9001 atccctggta atccgccaaa cctgctgcga ttaccgaaag gttgcccgtt ccagccacgt
     9061 tgtccgcatg cgatggaaat ttgtagtagc gcaccgccgc tggaagagtt tacgcctggc
     9121 cgtctgcgtg cttgctttaa accggtggag gaactgttat gaatgctgta accgaaggaa
     9181 gaaaagtcct cctcgaaatc gccgatctga aagtgcactt tgaaatcaaa gatggcaaac
     9241 agtggttctg gcaaccgccg aaaacgctca aagccgtcga tggtgtcact cttcgcctgt
     9301 atgaagggga aacattaggt gtggtagggg aatcgggatg cggtaagtcc acctttgctc
     9361 gcgccatcat tggtttggtc aaggcgaccg acggtcatgt tgcctggtta ggtaaagagt
     9421 tgctgggcat gaagcccgat gaatggcgtg ccgttcgcag tgatattcag atgattttcc
     9481 aggatccatt ggcatcgctt aacccgcgta tgaccatcgg cgagatcatc gctgaaccac
     9541 tgcgtactta tcatccgaaa atgtcacgcc aggaagttcg cgagcgcgtg aaggcgatga
     9601 tgctgaaagt cgggttattg cctaacctga ttaaccgcta tccgcatgag ttctctggtg
     9661 ggcagtgcca gcgtatcggg attgctcgtg ctcttattct tgaaccgaag ctgattatct
     9721 gtgatgagcc ggtgtcggcg ctggacgtgt caattcaggc gcaggtggtc aacctgctcc
     9781 agcagctgca acgcgagatg ggattgtcat taatttttat cgctcatgac ctggccgtgg
     9841 taaaacacat ttccgatcgt gtgctggtga tgtatctcgg ccatgcggta gaactgggga
     9901 cctatgatga ggtctatcac aatccgctac atccttacac cagggcattg atgtcggcag
     9961 tccccatacc tgatccggat ctggagaaga acaaaaccat ccagttactg gaaggggaat
    10021 taccgtcgcc gatcaacccg ccttccggtt gtgttttccg tacccgttgc ccgattgccg
    10081 gtccagagtg tgccaaaaca cgtcctgtgc tggaggggag tttcagacac gccgtttctt
    10141 gcctgaaagt cgatccgctt taaaagcaat aagggctgac agttgtcagc ccttattcac
    10201 gccaaaagcg attatttatt cccgccagat gatatggcac agtttgtgat ctttttcgcg
    10261 gcataacaga atgcgcgcga aaacatcgtt gatttcacca tcttcactgt ccgccagacc
    10321 aatcaccact tcggcaaaaa agtccgggtt caaatcgaaa tcaacatgtt cctgccagtc
    10381 ttctgccgga tcgaataact ccgcgccgcc gcgctcttca aactgaagat tgaacagcag
    10441 aacatcagct ggatcgagat tgtcggcagc cagttcgaga aaaatatcgt aggcctgctc
    10501 aagcgtttca tcttcagtca ggcgattgtt tagatccata tccatagtca ctacctgttt
    10561 aacctctgtt ggcgacgttt tacagcaacg ggctgaagaa gtaaaacagt cgctcggcga
    10621 cacgttgcca taatggacgt tttagccata aacgggcatc gagcagacgt gaacgcgaaa
    10681 tataatcgtc ctgaacggcg gcaaggtctg caccaaaacc tttatcgtcg attgccaggg
    10741 taatctcgaa atttagccac agactccgca tatcaaggtt aacggtgcca accagactta
    10801 gttcaccatc gaccagcacg ctcttggtat gcagtaaccc gccttcaaac tgataaattt
    10861 taaccccagc agccagcagt tccgtaaaga atgcgcgact ggcccagccg accagcatcg
    10921 agtcattttt tcgcggaagg ataatactga catccacccc gcgctgcgcc gccgtgcaaa
    10981 tcgcatgaag taaatcatcg ctgggcacaa agtagggcgt tgtcatgatc aaatattcac
    11041 gcgccgaata agcagcagtc aataatgcct ggtgaatgag atcttccgga aagccggggc
    11101 cagaagcaat tgtgtgaatg gtgtgaccgc tggcctgctc aaacggcata atattgacat
    11161 ctggtggtgg cggcagaata cgttttccgg tttcaatctc ccagtcgcag gaataaataa
    11221 tccccatcgc ggtagcgatg ggaccttcca tacgcgccat cagatcaatc cattgcccta
    11281 cgcccgcatc ttgtttgaag tagcgagggt cgaccatatt catgctgccg gtgtacgcga
    11341 tgtaattatc gatcatgatc atcttgcgat gttggcgcag gtccatacgg cgtaaaaaca
    11401 cacgcatcag attgaccttt aaggcttcga caacttcaat accggcatta cgcattagct
    11461 cgggccacgg gctgcggaaa aaagccacac tcccggcgga gtcgagcatc aatcggcaat
    11521 gaatgccgcg tcgcgcagcc gccattaatg attcagccac ctggtccgcc atgccgccgg
    11581 gctgccagat ataaaacacc atctcaatat tatggcgagc gagctggatg tcgcggatta
    11641 acgcctgcat cacatcatct gactcggtca tcagttgcag ctgattccct ttgaccccag
    11701 cgatcccctg acgacgctcg caaagcttga ataatggcgc agcgacactg ctattttctt
    11761 cggcgaagat atgcttacag gctttaaggt cgttaagcca ttttgcggtg gaaggccaca
    11821 tcgctctggc gcgctcagcg cggcgtttgc ctaaatggag ctcgccaacg gcaagatagg
    11881 caataattcc gactaacggc agaatgtaaa taatcaacag ccaggccatc gcggagggaa
    11941 ctgcgcgtcg tttcattaga atgcgtaaag ttacgcctgc aatgagcaac cagtatccca
    12001 gaatggccaa ccaactcacc aacgtataaa cggttgtcat agattagaaa tccttttgaa
    12061 agcgcattgt tatgagttta cgcatatgga ttcatctggc aaataaaaac gcgggaaaag
    12121 cactggtcag cgtcgggggc gggggtataa tgaccattct gttattgcat agagtagtta
    12181 acatgaagcg gagtagaacg gaagtggggc gctggcgcat gcagcgtcag gctagccgac
    12241 gtaaatcgcg ttggcttgag gggcagtcgc gccgaaatat gcgtatccac agcatcagga
    12301 agtgcattct aaacaaacag cgtaactcgt tattgtttgc gatctacaat atctaaatgt
    12361 actgggcacc attgcggtgc ccaataagtt tactcttccc tctttaaact ctatttttgc
    12421 gccgattctt tactgtcagc tttcgtttct tgttcatcgt tatcgctgaa aatgccatga
    12481 ccggagttca acaacattga aacgagcata tcattattaa tctcttcacc atttaaaact
    12541 cgcgccagaa tttcgctgcc aaacagttgc ggcgagagaa tgatatccgg atgtaccatc
    12601 ttaatcttat tcaggttttt gctatcactg acggcgagaa ctgttttgac atcactgctc
    12661 atatcttttg ccgagagtac aacaaacgcg ttatctgcat cgttatcact cagcgccaga
    12721 atggcccggc atcgatcaat tccagctttc tttaatactg aactgtcatt actgtcaccg
    12781 gggataacat cagcgttatc gcctaagcgt tgctcaagtt gcttgatatc atcttcaggc
    12841 aagttgctga taaccgttac gttttgtccg cgttgattca gttgcaggat cgtattgatg
    12901 gcgagaatcg aatgtccgca aacaataaaa tgatctttac gatgcattgt atgattgttt
    12961 ccttttacaa gtttgttgaa tcccccgcgg ataagcggac caaaaattga ggtcatagat
    13021 gtggcaaaaa cggtaatgcc ggaaataatg accgaaatag tgaacaatcg tgctgattca
    13081 gaaacaggga caatatcgcc atagccgacg gttgacatgg tttctatcga aaaatagaac
    13141 gcggtcatca aactttctat tcgcggatta aaaccttcgc ttaaataaag cgcaccgtag
    13201 gtggagtaaa acagtaacgt cgtgaaacta ataaatgcga aaattgtccc ggctgcggcg
    13261 ctactgtggg agaagtcttt gcgcagtatc agcaaaaaca ccagcgtaaa aatgcaaaat
    13321 ccaatactaa atttcagcca gggataaaaa tgcagggtgt aaattagcgc tatcaacaac
    13381 aaaatgatac tgattgccca tgcgagcttt gcccggaaca gtaagccaat ggagttcagg
    13441 acaagaaaga cgccgagcat aaagagtggg gcgtttgcca gcaaggagag atccaactct
    13501 gaaaatgctt tgatatggaa aatatctagc aaattgaccg acataccata gattgtttta
    13561 aaaataagca atccatttaa aaagacggcc agcgcgagaa tgtcgtgccg tagggtcacc
    13621 cataaattag ttgctgtttg tttgaatgta gcccagtgac tcactgatac ctctgtaacc
    13681 acaattttgt ttaattatct tagcgtgcac attttctgaa catacatgca gcgcgtgaat
    13741 gtgttaaaca atagtagatg actttatccg cagtaatcaa aggttttgag tgagcaactc
    13801 tgcgtcagga aagggcattt ttcctgaata tgctcacatc atataaagaa atacagataa
    13861 agttattatc tgcttgtggt ggtaaatgca atgactgacc tataaggaaa ggccaaacaa
    13921 gaacacggtt gctaaaaccg tgcccttaaa tattgaattt ctattcagaa cactttctta
    13981 aatggtttca ctgaaacgtg ttcatagact cctgccgcta cgtacgggtc agcatcggcc
    14041 caggcctgag ctgcttccag cgattcaaat tcagcaataa cggttgagcc agtaaatccc
    14101 gcagcccctg gatcgttact gtctactgct ggcattggac cggcggtcaa caatcgacct
    14161 tcatcatgca gtaactgtaa acgtgctaaa tgtgccggac gaacggaaag gcgtttttcg
    14221 aggctatcag ctttgtcttg agcgtaaata acatacaaca cgggcacaac tccttgttcg
    14281 ggaaagttgt aaagtacgtt atttgaaagg gcgaagatct gcaacggaaa gatgatgtct
    14341 ttgttaagac catttaaaaa gtaaggtcaa ttacgccaaa aatgacattt tcactgatcc
    14401 tgatcgtctt gccttattga atatgattgc tatttgcatt taaaatcgag gcctggtttt
    14461 tctactgaaa tgattatgac ttcaatgacc cttgatttac ctcgccgctt cccctggccg
    14521 acgttacttt cggtctgcat tcatggtgct gttgtggcgg gtctgctcta tacctcggta
    14581 catcaggtta ttgaactacc tgcgcctgcg cagccaattt ctgtcacgat ggttgcgcct
    14641 gcggatctcg aaccgccaca agccgttcag ccgccaccgg agccggtggt agagccagaa
    14701 ccggaacctg agccgatccc cgaaccgcca aaagaagcac cggtggtcat tgaaaagccg
    14761 aagccgaaac ctaagccaaa accgaagccg gtgaaaaagg tacaggagca gccaaaacgc
    14821 gatgtcaaac ccgtagagtc gcgtccggca tcaccgtttg aaaatactgc acccgcgcgc
    14881 cctacatcaa gtacggcaac ggctgcaacc agcaagccgg ttaccagtgt ggcttcagga
    14941 ccacgcgcat taagccgcaa tcagccgcag tatccggcac gagcacaggc attgcgcatt
    15001 gaagggcagg ttaaagttaa atttgatgtc acgccggatg gtcgcgtgga taacgtacaa
    15061 atcctctcag ccaagcctgc gaacatgttt gagcgtgagg tgaaaaatgc gatgcgcaga
    15121 tggcgttatg agccgggtaa gccaggcagt gggattgtgg tgaacatcct gtttaaaatt
    15181 aacggcacca ccgaaattca gtaagcagaa agtcaaaagc ctccgaccgg aggcttttga
    15241 ttattactca acaggtaagg cgcgaggttt tccttcagga tcaaccgcga catacttaaa
    15301 taatgcttct gtcgctttat agcgttgccc gatgggttca gacgcgactt ttttcaccca
    15361 cacttcaata ttaatgctaa ccgatgtcgt ccctttctgg acacagcgtg catagcagca
    15421 caccacatcg ccgaccgcaa ccggtcgtaa gaaagtcatt ccttcaaccc gcacggtcac
    15481 tacgcgaccg tgggcaattt ctttcgccag aatagcgccg ccaatatcca tttgtgacat
    15541 taaccaacca ccaaagatgt caccattggc attggtatcg gcgggcatgg ctaaagtacg
    15601 taaaacaaga tcgccctgag ggacgttatg tgttgtagac atggtaaaac cgacttaaaa
    15661 ggaaaatcac aggcgcgatg ctactatgat tttcctgagg agaacagagg agatacagcg
    15721 ccagccccga aggactggca gtctggttag gatttatctt cctgcggcat gtggcggtag
    15781 atatagatac cgcttaacaa tgtgaagatt aaggtaaggg cggtcaggcc aaagacttta
    15841 aagttgaccc aaatattttg cggcagccag aatgcgatgt agatgtttgc cagaccgcaa
    15901 aggataaaga aaacagccca ggccagattc agcttcgacc ataccggttg cggcagcgtg
    15961 agttctttac ccagcatccg ctgaattagc ggctttttca tcacccattg gctgactaac
    16021 agggcacccg caaacagggc ataaatgaca gtaaccttcc atttaataaa ctcatcattg
    16081 tggaagaaca gggtcaagcc accgaagacg accaccagaa caaaagtgat cagggccatc
    16141 ttctcaacct tacgaaagcg aacccagcta tatataagca caatcgccgt ggcgacgatc
    16201 agcgccgcag tagccgcata gatgtcataa atcttgtaaa acgcgaaaaa gacaaccagc
    16261 ggtaaaaaat caagaaactg cttcatttta cgattccgta atcaaatgct taaggttatt
    16321 ggcgaatcaa catatacagg cggaacagat agatgagcag tacggctgaa atcaggttgc
    16381 tcaaggtgtt cgccagtacg gcaccaattt ccggggttaa tgcggcaaaa gaagaggcaa
    16441 aaagcagcaa cagtgtcttt gccagcaacc agctcagtac tgcgggtgcc accagacgca
    16501 tattcgccca agtcagccgc atactgctac gcatcgaggc aaaaacgccc attttgtcct
    16561 gtaccaacat caccggtgcc agggccagta aaatggccat gataattccc ggaacgacca
    16621 ccagcatgat gccaatctgt actaaaaggg tagtcagaaa aatcagaata aataactttg
    16681 gcaatatcgg cgcactggca ccaatagctc gcagcgcact gactctctga cccgcagaca
    16741 ccagctggat aattaatatt acgcctccgg cgagaatggc gttaccgatt aatcctgaaa
    16801 aagtggacgc cgctgaagcc tgcagcaaaa tttgttgctg ttccggtgac atattctgaa
    16861 ccaggtcgaa caacccacta ctgccgctaa cgggcacgcc gtcattgagc tgcgcaagct
    16921 gtgcatcact gggtgagaaa acatgcccta acaccactgt gataaacgca catagcaacg
    16981 ataccaacag aatggtcata aattgattac ggaagaaatt tccggtgtca cggtatacgg
    17041 actgcgccgt gatagacatg cactctcctt gagttttatg caggtgttaa ttagcgggta
    17101 attgtaccct ggttaagcct cgggtggcag catcaggtct tgtatggaaa agcatatctt
    17161 tgtaaagcgg aggtaattgc tggccttcag acgtgggcaa atcgcggcaa gtggcgatcg
    17221 agttaatatt tgcgtagcga aaatatttaa aaattgattt aaatcacatt aaccaggatt
    17281 ctcaatgcaa cttctaaatt aatccagatc aataaatggt gaattatcat atgtaatgtg
    17341 atctatgtag gatcatttgt tactccaatg taggtatatt cgtcacgttt ttataaccat
    17401 aacgacggag cggatatgaa aaagttaaca gtggcggctt tggcagtaac aactcttctc
    17461 tctggcagtg cctttgcgca tgaagcaggc gaatttttta tgcgtgcagg ttctgcaacc
    17521 gtacgtccaa cagaaggtgc tggtggtacg ttaggaagtc tgggtggatt cagcgtgacc
    17581 aataatacgc aactgggcct gacgtttact tatatggcga ccgataacat tggtgtggaa
    17641 ttgctggcag cgacgccgtt ccgccataaa atcggcaccc gggcgaccgg cgatattgca
    17701 accgttcatc atctgccgcc aacactgatg gcgcagtggt attttggtga tgccagcagc
    17761 aaattccgtc cttacgttgg ggcaggtatt aactacacca ccttctttga taatggattt
    17821 aacgatcatg gcaaagaggc ggggctttcc gatctcagtc tgaaagattc ctggggagct
    17881 gccgggcagg tgggggttga ttatctgatt aaccgtgact ggttggttaa catgtcagtc
    17941 tggtacatgg atattgatac cactgccaat tataaactgg gcggtgcaca gcaacacgat
    18001 agcgtacgcc tcgatccgtg ggtgtttatg ttctcagcag gatatcgttt ttaattccgc
    18061 acaaaaatga ccccgtaatt acggggtcaa taaggacatg gtataaaggg tattatttct
    18121 tcgcttctac gccatcagtt tcagagcgaa ttaagaattt ctcagttgtt tgcggaatat
    18181 gctgaattag ccagtcggcc atgtgctttt catcatttaa aatcgcttcg ataatcggaa
    18241 ttgaggctgt atcaccggca ttttttgctg ctgctaagag tgaggtataa caggcgattt
    18301 caaattgctc gaagacatat ccgctaatag agcctttgac tatttcatca gaagggaata
    18361 taccaccgat tgactgccca agtgcagcca ttttactcat ggaatcttta atgactgaac
    18421 gtgaaatgtc attacgatca agaatagttt ccagttgaac aatctggttt ttggtttcac
    18481 taagatgttg ttcaatacga gcgcgtagtt caggataatt atctatacga ctggccatgg
    18541 attcaagcat tgattcggct tgcttttcca ttgcgtgggc gtcacgtaac cagtcatgat
    18601 aatgttcaat acgattcatg aaaatgtcct ctttataaaa tgactaaaag ttaaagttca
    18661 tatttcaggc tttattttcg gctttcttat ttacgttatt aagggccaga tcagtcaatt
    18721 tgatgtcggt ggccttttct tcttccaggg tctctttcag aagcttcgct gctttacgat
    18781 aacctaattg ttcagccagc gtcgctaatg tcccgtaact ggcaatctca taatgctcga
    18841 ctttctgtgc tgcggcaatc agtgcggcat cacgcacttc gtttttctcg gtactttcga
    18901 tgacctcatt agcttcttca ataagacctt ccattgccac acatttcatg cgcttaattt
    18961 tcagattcga ttctgattcc acaacttgat caatacgttc aatctgtcca tatgtttcct
    19021 cgaggtgcgc atgaaaagcc tgacttaatt tttcgtttga tgttgctctt gcgagttttg
    19081 ccagtgcccg ggttaattgt ttttctgcgc tgtaggtatc tgaaagcagg tgaataaata
    19141 catcttcaat ggtcttcata ttcatatttt tctccagtga aatcactgcg agccatacgg
    19201 cccgcagatg caattatctt tatcagctga tattaataat taacggtgaa tcaggatttg
    19261 cctgatttat taccaccgct ttgttgaccg ccttttttac ccgcttcaga tgcgcgttgc
    19321 ggatcatttt taaaattacc gccgctatgc tgaccgcctt tacggccagc gtcggatgcc
    19381 ttttcacggt cttcggcgaa atttcctgaa ccaccacgat gttcggccat gttatttctc
    19441 ccgttgcgtt gcattgtttc attaatatgg gtgttgtgtg tcgacactca ttaaaattag
    19501 tcgctaatga aaattagtca aattaagcgc aacgagaaaa aagagggaaa atatattatg
    19561 aggaacattc tggatatatt aacaattgcc tgaggaataa gtgacttaga gaaaattatt
    19621 gattttactg gtgttatgtt gcgggattaa tttgtttgat tatattactg ttgggcggaa
    19681 aaaagacgta agttgacgtt cgacaggggc aagcgaaacg gtaaaaagat aaataataaa
    19741 tgaatttagg atttttccag cttcgttaaa gaaagttaaa atgccgccag cggaactggc
    19801 ggctgtggga ttaactgcgc gtcgccgctt tcatcggttg tacaaaagct ttcagtgccg
    19861 ccagcatttt ctctggctca ttaatatgtt gctcgatgat tttaacgatg gccgaaccag
    19921 aaatcgcgcc cgcagctcct gcatcaatcg ctgcttttac ctgatccggg gcggaaatac
    19981 caaagccctg caatggaggc gcagcattgt attctttcag cttcgtgacc agatgattga
    20041 ggggtaacgc ggcgcggttt tctgcgccgg tcacgcccgc acgtgacaat aaataggtgt
    20101 atccacgacc gtaggaggct atctggcgca gcaaatcatc gtcggcattc ggcgggcaga
    20161 taaagatagg tgcaagatta tgacgcaacg cggcctggcg gaagggagcg gactcttcca
    20221 ctggcacatc ggcaaccagc accgaatcga cgccgacttt ctcgcactgg gcataaaact
    20281 catcaatgcc tttgttaaac accaggttgg catacatcag caggccaatg ggaatggtcg
    20341 ggtgtttctg gcgaatcagt gccagcattt caaaacattg tgccggagtc acacctgccg
    20401 caaaggcgcg cagagtggcg ttttgaatcg tcgggccatc cgccagtggg tcggagaagg
    20461 ggatacctaa ctccagcgcg tcagcaccgg cttcaattag cgtgtcgata attttcagcg
    20521 actgctcaat gcccggatca ccgagcgtga cgaaaggaac gaatgcgcct tctttgcgct
    20581 ccttcaactg ggcaaacaga gattcgtagc gttccatcag atttcccctc gtgctttcaa
    20641 aatatcgtga acggtgaaga tgtctttatc gccgcgaccg gaaaggttaa ccaccagtag
    20701 ctgctctttt tccgggtttt cgcgcatcat tttcagcgca tgggccaggg cgtgtgagga
    20761 ctccagcgcc gggataatcc cttcgtgcag gcagagcgtt ttgaaggctt ccagtgcttc
    20821 atcatcggta attgacacgt aatcagcgcg tccagtgctg ttaagatacg cgtgttgtgg
    20881 gccgacagac gggaaatcca gtccggcgga gatggagtaa gattcttcaa tctgcccgtc
    20941 ttcggtttgc atcatcggcg ctttcatacc gaaatagatg cccacgcgac cgtgttttaa
    21001 cggtgcgccg tgctcgccag tttcgatacc gtgaccacct ggctccacac caatcaggcc
    21061 gacgttggtt tcattgatga aatcagcaaa catgccgatg gcattcgaac cgccgccaac
    21121 acaggcgata acagcatccg gcaggcgacc ttctctttcc agaatctgcg ctttcgtttc
    21181 ttcgccaatc atccgctgaa actcacgcac aatggtcggg taaggatgcg ggccagccgc
    21241 ggtgcccagc atatagtgcg cggtttcata actgccggac cagtcgcgta gcgcctcatt
    21301 acaggcatct ttcagcgtcg cggaaccgct atgcaccggg atcacttccg cacccattaa
    21361 acgcatacga aaaacgttag gcgactggcg ttcaacgtct ttggcaccca tataaatgcg
    21421 acatttcagg ccgagcaggg cgctggcaag ggccgacgcc acgccatgct gaccggctcc
    21481 ggtttcggcg atgatttccg ttttacccat ccgcttcgcc agcaacgcct ggccgagcac
    21541 ttggttggtt ttatgcgcgc cgccgtgcag caaatcttcg cgcttgagat acagcgtggt
    21601 gttagtgcca gccgtaatgt tctggcattt ggtgagtgcg gttggacgcc cggcatagtt
    21661 tttcagcagg tcgttgaact gagcctgaaa ttcaggatct ttttgcgcgc taacaaaagc
    21721 ttcttccagc tggcgcagag caggcatcag gatttgtggc acgtacatgc cgccaaactc
    21781 accaaaatac gggttaagta atgttgtcat tgttcctttc cttaatatgc gcgcagcgtc
    21841 tggaaaaccg aggccaaaag acgtgcgtct ttgatgcccg gttgcgactc tacagcagaa
    21901 ttaaaatcaa gtccggcgca gccggtttgt gccgcttcca cgcagttatc tgcgcctaag
    21961 ccccccgcca gcagaacgtt gccaagcgat tgaccgttta acagtgacca gtcgaaacgt
    22021 tgaccgcttc caccctggcc gttgtctaaa acatatttat caacgtgctg aagctcgcgg
    22081 gcgggcaggg tttcaccgac gcttaatgct ttccagatgg caacgtgtgc tggcagagct
    22141 tcacgcagcg tatcgatata cagctgatct tcattaccat gcagttgcac tgccgccagc
    22201 gataacacct tagctttgtc caccacatcg gcaatatcgt gattgcggaa cacgccaaca
    22261 tactgcaacg gtgccgcagc catcacttcc tgcgcctgtt caacgttgac gcaacgcggt
    22321 gatgtcgcaa caaaaatcaa cccaccgtaa atcgcgcccg cgtcataagc tgctttagca
    22381 tcttgcccac gcgtcaggcc acatacttta ttctcaccca gcaatacccg gcgcacggcg
    22441 gcgttcaaat cgtcatgggc catcaacgcc gaaccaatca gaaaaccgtt agcgaagtgg
    22501 cttaactcgc gcacctgagc gtaagtattg atgccggatt cgctgattac cgtcacgttg
    22561 tgccccagtt tcggcgcaag ctcgcgggta cggttgagat caatcgacaa atcacgcaga
    22621 tcgcggttgt tgatgccaac gacctttgcc cccaatgcaa tggcgcgctc cagttcctct
    22681 tcattactga cttcggtcag cacacccatc tccagactgt gggcgacggc ggcaaggtgg
    22741 cgatattgtt cgtcatccag tactgaaagc attaataagc aggcatcggc ctggtaatag
    22801 cgcgccagat agatctggta agggtcgata ataaagtctt tacataaaat cggctgcggg
    22861 gcgatttggc tgacgatgga gaggaaatca aagctcccct gaaaatattt ctcatcagtc
    22921 agcaccgaaa ttgccgaagc gtaatttctg tagacggttg caatgtgtgc cggatcaaaa
    22981 tcatcgcgga tcacgccttt tgacggtgac gcttttttac actccagaat aaacgccgtg
    23041 cgtgcaccct gtagcgcatc ataaaaatgt cgcgtgctcg gctgaacctc attctgaaaa
    23101 ctggccagcg gttgctgctg tttgcgggct tctacccaaa tcgccttgtc tgcgacgatt
    23161 ttcgctaaaa cggtttgcat catttaccct cgtgccgcca gtgcggtgac tctgtcgtaa
    23221 gcggaaccac tgcgcagtac ctcaagaacg gtttgcgcat tggcttgcag atcttcatgg
    23281 ccatgcaggc gcattaacat ggcgacgttc gccgcgacgg ctgcttcatg ggcggcgtcg
    23341 cctttacctt gtaacaagcg tgttaaaatg tcacggtttt cttccggtgt tccgcctgcc
    23401 agttgctcct ggtggtaggg tgtcaggcca aaatcttctg cagttagctg atagctctta
    23461 atttcaccgt catgcagttc ggcaacgatt gtcggcgcgt gtaatgaaac ttcatccatc
    23521 ccgccgctgt gcactaccgc agcgcgttga taccccagca cgcgcaaggt ttcggcaatc
    23581 ggcagcacca gttccggact ataaacacca attagcgcca gcggcggatg cgccgggtta
    23641 atcaatggcc ccaacacgtt gaacagggtg cgggttttca gttgctggcg aaccggcatc
    23701 gcgtggcgga atccggtgtg atatttcggc gcaaagagga aacagacgcc taactcatcc
    23761 agcgcctggc gcgatttatc ggcgttcata tcaagattaa taccgaacgc cgccagcaga
    23821 tcggacgagc cggatttact ggagacgctg cggttaccgt gtttcgccac tttcagccca
    23881 caggccgcgg cgacaaacgc actggcggta gaaatattga tgctgttgct gccgtcaccg
    23941 ccagtaccga cgatatcggc aaacagataa tccgggcgcg ggaacggcgc ggcgttttcc
    24001 agtagcgcgg ttgctgctcc ggcgatctcg ttcgggtgct cgccgcgaat tttcatgctc
    24061 accagcgccg ccgccagttg ttccggcttc agctcgccac gcaccaccgc tgaaaacagc
    24121 tggtgacttt cttgttggct aagcgtctgt gcctgataca gtttttccag aatcggttgc
    24181 agcgtgttgg ttggctcaag tttctgctgc gcccaggcca gcgtttgttc cagcaggcga
    24241 gcgccctggg tagtaagaat ggattccgga tggaactgga aaccgcaaac gcgatctgca
    24301 tcgtgacgca ccgccatcac catgccatta aaatgggcgt tgatggttaa acctgccggg
    24361 atattactgc caaccagcga gtgataacgc gccaccggca atgggtttgt taatccggca
    24421 aacatcgcct gactgtcatg ttcaatgctc gacgctttac cgtgaagaat ttcgcccgcc
    24481 tgaccgacat agcccccgta agcttcgaca attgcctgat gtccgaggca aatgccaata
    24541 attggcagct tgccacgcaa gcgggtgagg agttccggca tacaaccggc ttcgctcggc
    24601 acaccggggc caggagaaag catcagcacc ggattgctca tcgtcgccag gcgttcaatt
    24661 aaggtctgcg ccggaacatg gttgcggtaa atcaccacgt tatgaccatt gctgcgcaac
    24721 tgatctgcca ggttgtacgt aaaagagtcg atattatcga gcagcagaat gtcagccatc
    24781 agaacgtctc ctgtgcatga tgcgcggtgg caatagcgcg cagtacagcg cgggctttat
    24841 tacgggtttc gtcggcttcc gactgcggaa cagaatcaag gactacgcca gcaccggctt
    24901 gcacggtggc gataccgttt tccaccagcg ccgagcggat cacaatgcag gtgtcgagat
    24961 cgccatgcgc ggtaaaataa cctaccgcgc cgccgtagct gccgcgtcga cgaccttctg
    25021 cttcggcaat taactgcata gcgcgcactt tcggcgcacc gcttaatgtc cccatattca
    25081 tacaggcgcg gtaagcgtgc agggcgtcga gatcgtggcg cagctcaccg accacgcggg
    25141 agacgaggtg catcacataa gaataacggt caactttggt gagatcggcg acgtagcgac
    25201 taccgggagt gcaaatacgc gccaggtcat tacgggcgag atccactagc atcagatgtt
    25261 cagaaagctc tttatgatcg gtacgcattt ccagttcgat gcggctgtcg aggtctctgt
    25321 ccagtgaacc atcggcacga cgaccgcgtg gacgtgttcc ggcaatcggg tagatctcaa
    25381 tctggcggct ggtggcgtca tacttgagcg aactttccgg cgacgcgcca aacagggtga
    25441 aatcattatc ctgcataaaa aacatgtacg ggctgggatt actctttttc agcacgtaat
    25501 aggccgccag cggtgacggg cagggcagag agaaacggcg agatggcacc acctggaaaa
    25561 tttctccggc gcgaatcgct ttttgcagcg aacgcactac gccaccgaac tcttcatcac
    25621 tctggttaca ttcacaacgc atatgcggca cggaaaccac cggcagcggc ggcgcggctt
    25681 cggtcagttg ctgacgtagt tcgttcaggc gagcagtgag acgttgtttt tcttcttcat
    25741 tcggagcaaa caggctggcc tgaatacggg tgcttttttt ctgatggtca atcaccatca
    25801 gcgtttcagc gagataaaaa cagaaatcag gacagttatt ttccgctgcc agttgtggta
    25861 aatcttcaaa tcccgccaca aggtcataag agaacagacc gccgaagaac attgcttctc
    25921 gttcttcatt cggtacattc aacagactct gcaataagcg gaaagcgtca aaaaccgaaa
    25981 gggagcataa gcgagcgtct tcatccagca gtggactgac aggggggaag cgcagcacac
    26041 ggcagtttgg tgattgttca ttttccacac ccgcaggcag ggcgttatcc agtagtgcca
    26101 gcagggcttc gccattgccg gaaagtgcct ggattgtgac agtgtcacct aaagctgtaa
    26161 tgcgcagcgc actgtctacc agcagcaggc tttttaaatc atctttgctg tcgatatctg
    26221 cggattccag tagcagcgtt gctggacgat cctcacacaa ctggtgaaaa agcgcggtgg
    26281 gattgtcgcg ataagcgcct tcgcaggtta gcagttcgag agtcggtttt tgtgtttgca
    26341 ttgttattct ctaattttgt tcaaaaaaaa gcccgctcat taggcgggct gggtatctga
    26401 ttgctttacg catggtgaat acactgcccg tttcaggaag tgcgccacca acctttcagt
    26461 acgaaaattg ctttcattgt cgataccctt tttacgtgaa cttgcgtact agttaactag
    26521 ttcgatgatt aattgtcaac agctcatttc agaatatttg ccagaaccgt tatgatgtcg
    26581 gcgcaaaaaa cattatccag aacgggagtg cgccttgagc gacacgaatt acgcagtgat
    26641 ttacgacctg cacagccata ccacagcttc cgatggctgc ctgacgccag aagcattggt
    26701 gcaccgtgca gtcgagatgc gcgtcggcac cctggcgatc accgaccatg acaccacagc
    26761 tgcaatctca ccagcaagag aggaaatttc acgttcggga ctggcgctga atcttattcc
    26821 cggcgtggaa atttccacgg tctgggaaaa tcatgaaatt catattgtcg ggctgaatat
    26881 tgatattact catccgctaa tgtgtgagtt cctcgcgcag cagacagaac ggcgcaatca
    26941 gcgggcgcag ctgattgccg aaagacttga aaaagcgcaa atacctgggg cactggaagg
    27001 cgcgcaacga ctggcgcagg gcggtgcggt gacgcgcggt cattttgctc gtttccttgt
    27061 ggagtgcggc aaagccagtt caatggcgga tgtctttaaa aagtatctgg cgcgcgggaa
    27121 aaccggatac gttccgccac agtggtgtac aatagaacaa gctattgatg tcattcatca
    27181 ttctggcggt aaggcggtgc tagctcatcc tgggcggtac aatcttagtg ctaaatggct
    27241 gaaaagattg gttgcgcatt ttgccgaaca ctacggcgac gcgatggaag tcgcgcagtg
    27301 ccagcaatcg cccaatgaac gcacccagct ggcgacactt gcgcgtcagc atcatttatg
    27361 ggcatcacaa ggatctgatt ttcatcagcc atgcccgtgg atcgaactgg gtcgtaaact
    27421 ctggctgccc gcaggcgttg aaggcgtctg gcagctatgg gaacagccgc agaacaccac
    27481 agagagggaa ttatgagcca gtttttttat attcatcctg ataacccgca gcaacgcctg
    27541 atcaaccagg cggtggagat cgtgcgtaaa ggcggggtga ttgtttatcc aactgattcc
    27601 ggctatgcgc tcggctgtaa aattgaagat aaaaacgcga tggagcgtat ttgtcgaatt
    27661 cgccagctgc cggacggtca caactttacc ctgatgtgtc gcgatctttc tgagctctcg
    27721 acctattcat tcgtggataa cgttgcgttt cgtttgatga aaaacaacac gccgggcaac
    27781 tacaccttca tcctgaaagg gacgaaggag gtgccacgcc gtctgttgca ggaaaaacgc
    27841 aaaaccatcg gtatgcgtgt gccttctaac ccgattgcgc aggcgctgct tgaagcattg
    27901 ggcgaaccga tgctttccac atcactgatg ctgccaggca gcgaatttac cgaatccgat
    27961 ccggaagaaa ttaaagaccg tctggaaaaa caggtggact tgattattca cggcggctat
    28021 ctcgggcaga aaccaacaac ggttattgat cttactgatg atacgccggt cgtggtgcgt
    28081 gaaggcgtag gtgatgtgaa gcctttctta taacaaattg cgttccacgg atggaagact
    28141 atggcaggga tatatcgctg cattttgctg ttgatagttg ggcttttttt ctcttcgttg
    28201 agttatccga aaaacacgga gatccctttt tatgaagaag ggatctcgct ctttgatgtt
    28261 gaagccactc tgcaaccgga tggggtgctc gacatcaaag aaaatattca ttttcaggcg
    28321 cgaaatcagc agattaagca cggattttat cgtgatttac cacgactctg gatgcagcct
    28381 gatggggacg ctgcactgct gaactatcat attgttggcg tcatccgtga tggtattcct
    28441 gaaccctggc atcttgactg gcatatcggg ttaatgagta ttgtcgtggg cgataaacaa
    28501 cgtttcttgc ctcaaggcga ctatcattat caaattcatt atcaggttaa aaatgctatc
    28561 ctgcgtgagg gggattctga tctgctaatc tggaacgtga ccggtaacca ctggccgttt
    28621 gaaatttata agaccctatt ttcactcaag ttgcctgata ttgcgggtaa tccatttagc
    28681 gaaatcgatc tctttactgg agaagagggc gacacatatc gaaatggccg catccttgag
    28741 gacggaagaa ttgaatctcg cgatccgttt tatcgtgaag atttcacggt cctctaccgc
    28801 tggcctcacg cattacttgg taatgccccg gctccacaaa cgacgaatat tttcagtcat
    28861 cttcttttac cctcaacgtc atcgttgtta atttgctttc cgagtctgtt cctggcgtgt
    28921 ggatggttat atctctggaa gcgcaggccg caatttacgc cggtagatgt gattgaaacc
    28981 gatgtcattc cgccagatta cacgcccggc atgttacgtc ttgatgcgaa actggtttac
    29041 gacgataaag gtttttgcgc cgatatcgta aatctgattg tgaaagaaaa aattcatctg
    29101 gaagatcatt atgacaagaa ccagcaaatc ctgattcgtg ttaatgaagg cgcgaccaga
    29161 aataatgcgg tattactgcc cgcagagcag ttattactgg aagcgttatt tcgtaaaggc
    29221 gataaggtcg ttcttacggg gagacgcaac agagtcttac gcaaggcatt tttacggatg
    29281 cagaaatttt atctgccgcg taaaaagtct tcgttttacc gacctgatgc gtttttgcaa
    29341 tggggcggaa tggcaatatt ggcggtcatt ctctacggta acctgagtcc cgtaggctgg
    29401 gaaggaatga gtctggtggg cgatatgttt attatgatct gctggcttct tcctttttta
    29461 ttttgttccc ttgagctttt gtttgcccgc gatgatgaca agccttgcgt taatcgtgtc
    29521 atcatcactt tgtttttacc actgatttgt tcaggtgtgg ctttttactc tctctacatc
    29581 aatgtcggag atgtattctt ttactggtat atgccagcgg gttattttag cgctgttttc
    29641 ctgaccggtt atctcactgg catggggtat atttttctgc caaagtttac ccaaactggg
    29701 cagcaacgtt atgcccacgg tgaagctatc gttaactatc ttgcgcgtaa agaggcagca
    29761 acacacagtg ggcggcgtag gaaaggggaa acacggaaac tggattacgc tttgttaggt
    29821 tgggctatat cggcaaattt ggggagggaa tgggcgttac gcatcgcccc ttcgctttct
    29881 tcggcgattt gcgctccaga gattgcccgt aacggcgttt tattctcatt acagacgcac
    29941 ctgagttgcg gggcctatac cagtttgttg gggcggagct attctggtgg tggttctggc
    30001 gctggtggcg gaggcggtgg tggctggtaa tcaagctgat gttaatcgaa acaagcttta
    30061 accataaaga tagtgtagta tgttgcgcct caaagccagg caagttaaaa cgtcgagtcg
    30121 tttacttaag gcctgaagag ttcaaacaga ctttactatc agtgagtcag atgtaaattc
    30181 tgactcactg ttgcacaaaa gcatatcaga cgcctgggaa ggcgacacca aaaggaagct
    30241 ctatgagcga aaagctacag aaagtgctgg cgcgtgccgg ccacggttct cgccgtgaaa
    30301 tcgaatctat tattgaagcc ggtcgtgtga gtgttgatgg caaaattgct aaactcggcg
    30361 atcgcgttga aattacccct ggattgaaaa tccgtatcga tggtcacctg atttcggtac
    30421 gtgagtccgc tgaacaaatt tgtcgcgttc tggcctatta caaaccggaa ggtgagttgt
    30481 gtacccgtaa cgatccggaa gggcgtccaa cggtgtttga ccgtctgcca aagctgcgtg
    30541 gcgcacgctg gattgccgta ggtcgcctgg acgttaatac ctgtggtctg ctgctgttca
    30601 ctaccgacgg tgaactggca aaccgtttaa tgcatccaag ccgtgaagtt gaacgtgaat
    30661 acgccgtgcg cgtatttggt caggttgacg acgcgaaact gcgtgatttg agtcgtggcg
    30721 tgcagctgga agatggtccg gcagccttta aaaccatcaa gttcagcggc ggcgaaggga
    30781 tcaaccagtg gtacaacgtt actctgaccg aaggccgtaa ccgtgaagtt cgtcgtctgt
    30841 gggaagcggt tggtgtgcag gtaagccgcc tgatccgtgt tcgttacggt gatatcccac
    30901 tgccgaaagg tctgccacgc ggtggctgga cggagctgga cctcgcccag actaactatc
    30961 tgcgcgaact ggtggagcta ccgccagaaa ccagctctaa agtcgctgta gaaaaagacc
    31021 gtcgtcgtat gaaggcgaat cagattcgtc gtgcggtgaa acgtcactgt caggtgagcg
    31081 gcggtcgtcg ttctggtgga cgtaataata acggttaatt taagttaagt ataaaataaa
    31141 ggccctg
//
LOCUS       DAZGHF010000047        30861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGHF010000047 DAZGHF010000000
VERSION     DAZGHF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.047"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(135..698)
                     /gene="fimA"
                     /locus_tag="ACHK3A_002257"
     CDS             complement(135..698)
                     /gene="fimA"
                     /locus_tag="ACHK3A_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO8740325.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(1132..1398)
                     /locus_tag="ACHK3A_002258"
                     /pseudo
     CDS             complement(1132..1398)
                     /locus_tag="ACHK3A_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1674..2990)
                     /gene="hipA"
                     /locus_tag="ACHK3A_002259"
     CDS             complement(1674..2990)
                     /gene="hipA"
                     /locus_tag="ACHK3A_002259"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO8740326.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPQVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREWY"
     gene            complement(2990..3256)
                     /gene="hipB"
                     /locus_tag="ACHK3A_002260"
     CDS             complement(2990..3256)
                     /gene="hipB"
                     /locus_tag="ACHK3A_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO8740327.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(3449..5338)
                     /locus_tag="ACHK3A_002261"
                     /pseudo
     CDS             complement(3449..5338)
                     /locus_tag="ACHK3A_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            complement(5488..7080)
                     /gene="lsrK"
                     /locus_tag="ACHK3A_002262"
     CDS             complement(5488..7080)
                     /gene="lsrK"
                     /locus_tag="ACHK3A_002262"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO8740328.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDPEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVNELKELHNNTFENKVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLIDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(7159..8112)
                     /gene="lsrR"
                     /locus_tag="ACHK3A_002263"
     CDS             complement(7159..8112)
                     /gene="lsrR"
                     /locus_tag="ACHK3A_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO8740329.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            8361..9896
                     /gene="lsrA"
                     /locus_tag="ACHK3A_002264"
     CDS             8361..9896
                     /gene="lsrA"
                     /locus_tag="ACHK3A_002264"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO8740330.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSIQKMNNLLVALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFRRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINRLSTGD
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            9890..10918
                     /gene="lsrC"
                     /locus_tag="ACHK3A_002265"
     CDS             9890..10918
                     /gene="lsrC"
                     /locus_tag="ACHK3A_002265"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO8740331.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYVTGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTVLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            10918..11910
                     /gene="lsrD"
                     /locus_tag="ACHK3A_002266"
     CDS             10918..11910
                     /gene="lsrD"
                     /locus_tag="ACHK3A_002266"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO8740332.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVAVYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            11922..12944
                     /gene="lsrB"
                     /locus_tag="ACHK3A_002267"
     CDS             11922..12944
                     /gene="lsrB"
                     /locus_tag="ACHK3A_002267"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO8740333.1"
                     /translation="MTLHRFKKIALLSALGIAVISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            12971..13846
                     /gene="lsrF"
                     /locus_tag="ACHK3A_002268"
     CDS             12971..13846
                     /gene="lsrF"
                     /locus_tag="ACHK3A_002268"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO8740334.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKAGIPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            13870..14160
                     /gene="lsrG"
                     /locus_tag="ACHK3A_002269"
     CDS             13870..14160
                     /gene="lsrG"
                     /locus_tag="ACHK3A_002269"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO8740335.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            14217..14975
                     /gene="tam"
                     /locus_tag="ACHK3A_002270"
     CDS             14217..14975
                     /gene="tam"
                     /locus_tag="ACHK3A_002270"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO8740336.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STTLLHQRWPAARITGIDSSPAMIAEARSTLPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQSIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(14979..15893)
                     /gene="yneE"
                     /locus_tag="ACHK3A_002271"
     CDS             complement(14979..15893)
                     /gene="yneE"
                     /locus_tag="ACHK3A_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO8740337.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISVVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(16090..17541)
                     /gene="uxaB"
                     /locus_tag="ACHK3A_002272"
     CDS             complement(16090..17541)
                     /gene="uxaB"
                     /locus_tag="ACHK3A_002272"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO8740338.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTGLVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILKKGMREAVRPLC"
     gene            complement(17769..19187)
                     /locus_tag="ACHK3A_002273"
     CDS             complement(17769..19187)
                     /locus_tag="ACHK3A_002273"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO8740339.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(19326..19685)
                     /gene="yneG"
                     /locus_tag="ACHK3A_002274"
     CDS             complement(19326..19685)
                     /gene="yneG"
                     /locus_tag="ACHK3A_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO8740340.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(19685..20611)
                     /gene="glsB"
                     /locus_tag="ACHK3A_002275"
     CDS             complement(19685..20611)
                     /gene="glsB"
                     /locus_tag="ACHK3A_002275"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO8740341.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRMGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCIELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(20675..22063)
                     /gene="sad"
                     /locus_tag="ACHK3A_002276"
     CDS             complement(20675..22063)
                     /gene="sad"
                     /locus_tag="ACHK3A_002276"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO8740342.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRVRSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNNGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETRARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            22164..23039
                     /gene="yneJ"
                     /locus_tag="ACHK3A_002277"
     CDS             22164..23039
                     /gene="yneJ"
                     /locus_tag="ACHK3A_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8740343.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAATKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPAMLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQS"
     gene            23123..23242
                     /gene="yneK"
                     /locus_tag="ACHK3A_002278"
     CDS             23123..23242
                     /gene="yneK"
                     /locus_tag="ACHK3A_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO8740344.1"
                     /translation="MVTPVSISNFISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            23384..24574
                     /gene="ydeA"
                     /locus_tag="ACHK3A_002279"
     CDS             23384..24574
                     /gene="ydeA"
                     /locus_tag="ACHK3A_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO8740345.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGELITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(24599..25264)
                     /gene="marC"
                     /locus_tag="ACHK3A_002280"
     CDS             complement(24599..25264)
                     /gene="marC"
                     /locus_tag="ACHK3A_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO8740346.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            25476..25910
                     /gene="marR"
                     /locus_tag="ACHK3A_002281"
     CDS             25476..25910
                     /gene="marR"
                     /locus_tag="ACHK3A_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO8740347.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            25931..26314
                     /gene="marA"
                     /locus_tag="ACHK3A_002282"
     CDS             25931..26314
                     /gene="marA"
                     /locus_tag="ACHK3A_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO8740348.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            26346..26564
                     /gene="marB"
                     /locus_tag="ACHK3A_002283"
     CDS             26346..26564
                     /gene="marB"
                     /locus_tag="ACHK3A_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO8740349.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPTSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(26595..27494)
                     /gene="eamA"
                     /locus_tag="ACHK3A_002284"
     CDS             complement(26595..27494)
                     /gene="eamA"
                     /locus_tag="ACHK3A_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO8740350.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            27689..28876
                     /gene="ydeE"
                     /locus_tag="ACHK3A_002285"
     CDS             27689..28876
                     /gene="ydeE"
                     /locus_tag="ACHK3A_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO8740351.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYNLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRNEKIIATETGSAWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            29003..29098
                     /gene="mgtS"
                     /locus_tag="ACHK3A_002286"
     CDS             29003..29098
                     /gene="mgtS"
                     /locus_tag="ACHK3A_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO8740352.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            29098..29202
                     /gene="mgtT"
                     /locus_tag="ACHK3A_002287"
     CDS             29098..29202
                     /gene="mgtT"
                     /locus_tag="ACHK3A_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO8740353.1"
                     /translation="MNGDNPSPNRPLVTTVYKGPDFYDGEKKPPVNRR"
     gene            complement(29317..30207)
                     /gene="dgcZ"
                     /locus_tag="ACHK3A_002288"
     CDS             complement(29317..30207)
                     /gene="dgcZ"
                     /locus_tag="ACHK3A_002288"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO8740354.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYELCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(30462..30854)
                     /gene="ydeI"
                     /locus_tag="ACHK3A_002289"
     CDS             complement(30462..30854)
                     /gene="ydeI"
                     /locus_tag="ACHK3A_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO8740355.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         7794 a         7827 c         7335 g         7905 t
ORIGIN      
        1 tcataccgct ccaaaacaca aactattcct cattaataat gagatgaaca ttggtgaatt
       61 tctcgggtgt gcgtagccat gccacacaca ccctgtttca atttcagatg acgtatagcc
      121 atcttaagat tgaattatag atattgaact ttgaaggtcg catcagcgtt tgcagtacca
      181 gctactgact gcccgagagc aatataacga gcctggaacg gcaatatatt cgtgccatca
      241 atcaagtcag ttttagcgct aaaagtggca ccatcaagta ttaatacctc ccccgtacgg
      301 tccaaaattt gaataccgac attttgggca gagcctgccg ctgaactctg cagcgctaac
      361 gtatcgtcat tactggtgac agtagtaccc aggaatgcaa cagctgcatt actggaaaca
      421 ttggtatcac aatcattcag cttgatattg aagccaacgg cggagctgag gtcacccgct
      481 ttagccagac gagaagaacg gacctgaccc agctcaaccg tttggttcat tgattctgaa
      541 tcaatcgcac aagcggcatt taccacttca cctttaaaat gaataacacc gccattaaca
      601 gtggtcgtta ctgattcatc accctcggct gcgcttaccg cagcagacga catcgccaat
      661 acagaaacga caatcatacc gacatgtttg agtttcataa ttctccaaaa tttatattta
      721 agtagctgaa taaagagagt taacaattaa gtcattctgt tattgcttat aaaacaagaa
      781 tcattcttaa ttggtagttg ccctgagaat atagtttcca gggttattac atttcacgaa
      841 gaacgataat atctttaaat tacaaggaca cctaatcaca acaagtttat tgcagcactt
      901 gttgtagaga gtatttgcaa agacatttta tgtcgctaaa taacatgacg atttcattat
      961 gcatgttatt tataaaccgt gactaaacag aatattattg cgaacaaggg aagtgtcagg
     1021 aaaggtcaag agcgaccaat ggtttatatg gctataaatc attagccata gttaatgttt
     1081 gcctggtgta actaacacgg aattgctcta aacagataag tgtagggttt attaaagctg
     1141 accgacagac tttcttaacc tggtaatatc tctcttaggt gactctccaa acatccgcga
     1201 atattcccga ctgaaatggg ataagctttc gtaaccgaca gcataggctg cagtggtgac
     1261 atcgtaatgt tcattcagca tcagtcgcct ggcttcattg aggcttagcc atttccgata
     1321 ccggagtgga ctcattcccg caagctgtct gaaatgttga tggaaagagg gggtgttcat
     1381 ttgcacgcgg tttgccagcc tgactcttca acgctgagaa gatgcggcaa acggcaaacc
     1441 tgcataccag ccagcctata tccggtatgc acaggccaat tatgaaagag gagaggcttt
     1501 atgttctgga accttgaagt aataacgtcg accggaaaat cactctgcat catcactcag
     1561 attcaattta acataatata cattatgcgc accaacataa accaagggag aatccagccg
     1621 ttggcggtca tgattgtcct gctcattaac aacggccaca ccccatatct tacttaatac
     1681 cattctcggc ttaaccgacg gtgcaacctc aacacattgg tttcaactgc cgtcaccacg
     1741 ttctccggaa aatctgtcgg taatgaagtc ttcacgttat ccagtgctgc cggaatcatt
     1801 ctggcaaagt cactcaggat ttcatgcatc tgcacttgcg gaaatttcag cacctttgct
     1861 gtcgccagaa aatgtcgcgg ataaatttta tcgattgccg tttttttgcc tttggatgcg
     1921 ttaagcccca ttgccagttt gagatcgctg atgtgtattc ctgcaccacc caataccgga
     1981 aatgctgaaa tgatgtcgta aaatggcgtg agtcgataac tgccgccagc ctgaataaat
     2041 acggagaagt tttttgcatg gccatccgtt gcgccaatca accactggaa cacctgaaac
     2101 ttcataaaat catagcgatc tttcagcgcc tcgctggacc ccatcaaaaa agccatgatc
     2161 cgcgcgatgc ctgggcctcc atctgattca tatttcaccg atgaaggtaa accgaatgtc
     2221 tgacacatat cctcctgtgg caagcgaagt aaaaccgttc gctcagcatt ccaacgcctg
     2281 tcaaaacgtt cgaccgctaa cgcgcgcaca tttcccgctt taatgatttc tgcgtccggc
     2341 acattcaacc caagttcttt agccagcagc agacagtaat actcattatc aacgctttgg
     2401 ctgagatcga gcgtcgcatt gggctgcctg atttcgccaa tcggtaattt aatgatgtgc
     2461 gtcgtcggcg ttattccttt cggaatgcac cagtcattgc ctattctgag cagcgctgtc
     2521 ttctcctgcg cgccagcaac cgagatgcga aagtcatttt cttctctaat catgcccaga
     2581 gggatatctg ctttataagc cgttaatact tcttcaagcc tggcttcagt gagctgttcc
     2641 catgccatta tcgggcgcgt tatggtttca ttttcgggta ataacgttac ggcaccaacg
     2701 ctgtctcgcc ctatttctga caataaatca aacggttgtc tggatttagc atgataacgt
     2761 ttaacgatcc gatcacgtac aatcgggcta tcgggtaaca ggttatcgaa gaagttaaat
     2821 acggcatcag aggtgatatt ccccctctgc aatggcagcg aaagtgacaa cggtctggca
     2881 taacggctgg ctaaccactc cggtgcatac ttaaaggtgt gcgcgccatt ggctaacttc
     2941 gttaactcgc ctacccgctg gttgttcatc caagtgacaa gtttaggcat taccactcca
     3001 gatcttgctg ttctgttgat tctggcgagg catttttcgt gtcgcatagc gtcattgaga
     3061 gttcaagcga ctgtaaaatc ttaaaaaagg tcgtcagcgt ggtattgtca gggttgtttt
     3121 cgaaattgga aatcgtcgcc tgcttaatac caattttttt cgccagctcg ctctgcgtcc
     3181 agccattttg ctggcgaacc agtttcattg cattcgccaa ttgcgttggg ctatagatct
     3241 tctgaaagct catcatgtca tacgtccaca gcaaatttat ccgcttaagg ggatattatc
     3301 agttttatcc tttagtgagg ataagtcaat ctggaaacac atcttatccg ctctacggga
     3361 taaacactgt gatatccgcg atcgcggata aaaaggagga tttaaaagtc gtttgaagtc
     3421 ggcaatatta agccgcatgc atcttcgaca tgcggctatt cccttgggta tcgcctttta
     3481 cgttcatctt atcggtgggc gaattatcgc cgcccagcgt cgcgttgaac gtcatcaggc
     3541 cattgtttcc ggtatagtta ccgttaacgg tcagtacatt ccccggtttc cccccttcgc
     3601 tgttcatttg aaccgtgcca ccattctcca ggttaccgcc gacagtggtt ttagcgacac
     3661 gcagtgtccc ccctggcata acgttcacat cacctgcagt ggtgacattt cccgacatca
     3721 caccgctgtt atggatatta atgtgctgac ttgccatatt aatggcagag tcggaaccga
     3781 aagcaatttc accacccacg atatccgtcg tacccttata cgccgcggaa tctttgttaa
     3841 gcgtcaggat cccggtaccc agttttgtca catcaccgct accgctgata ctgtgcgtca
     3901 tcacccagct acgctcagtg gagatggtga gcaggccatt attgataacg gaagcatcac
     3961 ccaggttttt ctgctcgttg acgctaagcg cgctgcctgt atcaatgttg aattgcccca
     4021 caaagcggct attatcgcca tccagctcta cgtctgtcct ggcggttgcg ctcacgatac
     4081 ccttcccgct tatgctatta cgcagttcgc cggtcacatt ttttagcgtt aacagaccgt
     4141 cattggcaat atttcctctg cctaacccct gcgtattatc gaggctgact tccgcatttg
     4201 ggctaatgct ggttaacgca ttgtagcgtg ccttaagccc ctcgatagcc agtgttcccc
     4261 ctgtaacatt caggtttccg ccacctgtca gttcgccctg actgatccca cctttattca
     4321 ccgtcagcga cccctcttta aacaaaacag tcgaacccat ttgcccggtg aatgtctcta
     4381 ccgtctgggt cgatccatta agatcgacga tggccgcgtt gctgatgttc agttcccggg
     4441 tgttgcccag cgcgccatcc gcatcggtac gtaatgtccc catctgaacg taggttgccc
     4501 cctgatagtc gttctgaccg ttggaaagcg aaacctgtcg taccgtattg attgccagat
     4561 ccccttcgcc accgattttt gccgacatat cgaccgttgc gccataagct ccgccatgtt
     4621 cggctaacgt cagcttttgc ccaccatgga tgttcagcgc tttcagccca tagttaacat
     4681 acaaaccatc accaggtgcg gtcgtgaggc ggaatccata atcaccctcg gcgaccaccg
     4741 ttccgttttg cgcaatatgt aacgttttgt ccgcttccac ctcgtcgccc tgtaaatcac
     4801 gtaacgttaa tgaaccaccc gatccaataa ccgtttgcgc cttcaccagt tgaacgccga
     4861 catggctatc gtcgtgttcc atcaaattga gcgtcgtcag agggttattc gccatgggat
     4921 cattccacgg tttaggcacg tcgataagca cgtcgcccat cccgtttacc tgatagttac
     4981 ggcctttcca ggtgtagtca cccgcgccga caaccagcgt atcgacgctg atatatccct
     5041 ccgcaagcgt cgcagcaggt atatccgtat cgaacgtgag cgtaccgcca ttcatggcta
     5101 gtccaccaat ggattgctct cccactttta ccgatgtggt attttcactg tcagactgca
     5161 gcatcgcgta agtaagcgca gcggtgttgt cgcgttccag tgcgaaggta ctggctttca
     5221 gttgggcaac accggcgaat tcagtccctg ctgcatgggt aaagccaaac attttgtcgg
     5281 aggatgacag ctgcactcgc atcaagccat cgcctttgag cgcattgatc agcgtgaata
     5341 gcaatttccc cttgaatata gtactggtga tggaacgatg aatctgcaag ctggcattta
     5401 acaagccagc tctaaaagaa gggaaatgag aataccaata ctcaaaaact aacagccacg
     5461 gtcatcatgg tgtggctgtc aatgaaacta taacccaggc gctttccata acgacgtcgt
     5521 cagtccatga tcaaccagcc ccagctgatc ctgataaact gcctgccact tatcgcgtga
     5581 atcctgataa agttcatgct tttccgggtc tggtgtgtgc gtccgttccc aacgaaccaa
     5641 gcgttctccg gtttctgcca ttgatgaaaa aattccggca ccgacgccag ctgcaatggc
     5701 acatcctaat gcagtggctt ctttgaccac cggaatattg acgggtaatc ccgagacatc
     5761 agcgagaatt tgactccata atttcccttt tgaacctccg ccagcaaaaa ccagcgaggt
     5821 gggatgaata ttcgagaaat cagcaatttg ctgtaagtta cacgctgata caatcgccgc
     5881 attttcttcc agcgcacgga acaatgtcgc tttgttacat ttatccgggt caatggacaa
     5941 gttaataaag gaaggcgcag cgtgatacca ggttttaaag cgcattctgt cggagaatat
     6001 cggcattacg ccccacgacc caggcggcac ccgactggcc atctcttcca gcagcgtata
     6061 ggtgtcgatg cctaaacgtt cggcaatcag tttttcttcg gcacagaaag catcgcggaa
     6121 ccagcgcatg gtgagtccgg taaaaaagct tatagattca gcttgtacca tgccaggaat
     6181 aacatgggga ttaacgcgca cgttcatttc tgggtctgtc accggcgcgg ctaagtttac
     6241 aacttgctgc cagaatgtgc cgccaagaac cgcggtttgt gccggacgta caacgcctaa
     6301 ccccaggcaa ccaagctgca cgtcacctcc tccaacgacc accggagtgc ccgccttcag
     6361 accgcagagt tccgccgctt gtgaacttac cacacccagc aatgtgccgg tttctttgac
     6421 aggagaaaga atatcggcac gcaggccagc catatccagc aatgcaggtt tccagtcacg
     6481 ggtggttaga tcaataagtc ccgtggtgcc agcgttagag ggatccaccg caagttcgcc
     6541 gctgagcata taggccagcc agtcgctgat catggtgatg gttgatgcct gacggtaaat
     6601 atcggaacga tggtgcgcca gccaaagtaa tctggggatg gcgcttaaag ccagcgtttg
     6661 cccggtcgct cgataaactt tgttttcaaa ggtattgttg tgcagttctt taagttcgtt
     6721 aacttcgcgc gccgctctgg catccacatt ggcgcaggcc cagatcggtg ttccttcatt
     6781 gttatataaa acaatgcctt cacgcatcga acatgccgaa acagcagcga tatactccgg
     6841 ggctatgccg gcgttgtgca gcgcctggcg catacactca cacgccagtt gccagttttt
     6901 gttgagatca aattccatag aaccaggaac gtccggtact gccagatgcc gccactccgc
     6961 ctgtcccacg gctatttgat tgccttccgg gtcgaatatc acagcccgaa tacttccggt
     7021 gcctgcatcc agcgccatca ggtagtactt tgattctgaa ggggtaaaga gtcgagccat
     7081 tagattatcc tcggcttatg cgttctgtct gttcctctat acgttctcca tcattcccgg
     7141 taacaaggtc atgcaaattt aactacgtaa aatcgccgct gctgtgtcct gatcggtaac
     7201 cagtgcgttg atatagccgc ctttcattgc agcggcaatg gcttcggctt tattttctcc
     7261 ccctgccacg ccaacccgga cgggtatggt cttcagcgcg cttaaaggta agccaatcag
     7321 ttcgttatgt attttaatat ccgtgacaac gtcacctttt gcatcaaaaa agtagcctaa
     7381 aatgtcgcca acagcccctt ttcgaccaat cattaactgt tcgccctggc tgatatagcc
     7441 ggagcgaatg attgttgcgt cgtcttgttg actcacagcc ccaatgccga caatcgccac
     7501 atccgctgct tgcgcggcta gcagaacatc tttgacgcaa ttttcatttt ttagcgtacg
     7561 ggcaatgtca gcggaggatg cccgcaacgg agccggaata atattcacac tgcacgccgc
     7621 gttaagctgc ccgatacccg tcatataaga accgactcca ccggagagcg tgaccaggcg
     7681 aatttgctgt gacgaaataa aaccacttaa gcgttgcagc gtattcatag ttgcctcgcc
     7741 aaaaccaatc gccagcatct gttgtggttg aagtaaactc atcaacatat gcgccgcgcc
     7801 tatccctagt cgcccgccga catcagcatc cgcaagccct gggatcaccc ggacatgttg
     7861 cagcgaaaac tgacgacgta attgtgtttc atattccaga cagccttcaa agcgagaatt
     7921 aatctgtacg cgaataatgc cggattgatg ccctttctcc agtaatcgcg acactttcaa
     7981 acgggtcagg ccgagacgat cgctgatctc actctgggtc agcccgtcgt ggtagtaaaa
     8041 ccacgcaatc cgcgcgacct gttcttcttc acacattccc tgttctgaaa ttaccgaatc
     8101 gttgattgtc ataattcatt cttcactttg aacatattta aatctttaat gcaattgttc
     8161 agttcttgcg catttatatc tgtgatagca accacagttt gactctacga gcatgaacaa
     8221 acgcaaccgt gaaaatcaaa atagcataaa ttgtgatcta ttcgtcggaa atatgtgcaa
     8281 tgtccaccta aggttatgaa caaattaaaa gcagaaatac atttgttcaa aactcacctg
     8341 caaaactgaa cgggggaaat atgcaaacga gtgatacccg cgcgttaccg ctactttgcg
     8401 cccgctcggt ttataaacag tattcagggg ttaatgtcct gaaaggcatc gattttacgt
     8461 tgcatcaggg ggaggtccac gccctgctcg gcggcaatgg tgccggtaaa tcgacgttaa
     8521 tgaagattat tgccggtatt acccctgctg atagcggtac gctggagatt gggggcaata
     8581 attacaccag attaacgcca gttcatgctc atcagctggg catttatctc gttccccagg
     8641 aaccgctgct ttttccaagc ctgtcgataa aagaaaacat cctgtttggt ctggcaaaaa
     8701 aacaactctc catacagaaa atgaataact tgctggtggc gctgggctgc cagtttgatc
     8761 tgcatagtct ggcaggatcg ctggatgtcg ccgatcgcca aatggtggaa atcctccgcg
     8821 gcctgatgcg cgactcgcgg attctgatcc tcgatgaacc tacagcctcg cttacccctg
     8881 cggaaaccga acgtttgttt cgtcgcttgc aagagctgct tgctactggc gtgggtattg
     8941 tttttatctc gcataagctg ccggaaattc gccagattgc cgatcgaatt agcgtgatgc
     9001 gcgacggaac catcgcctta agcggcaaaa ccagcgaact gtctaccgac gacattattc
     9061 aggccatcac gccagcgcta cgggaaaaat cgctctctgc cagccaaaaa ttatggctgg
     9121 aattacctgg caaccgccca caacatgccg cgggaacgcc ggtgctgaca ctggaaaatc
     9181 tgaccggcga aggtttcagg aatgtcagcc tgacgctcaa tgccggagaa attctgggct
     9241 tggctgggct ggtgggagcc ggacgcacag aactggccga gacgctctat ggtctgcgta
     9301 ctttgcgtgg cggacgcatt atgctgaatg gtaaagagat caatagatta tccaccggag
     9361 atcgtttact gcgcggtctg gtttatctgc cggaagatcg ccagtcatcc ggacttaatc
     9421 tcgatgcttc actggcgtgg aacgtctgcg cccttactca taaccttcgt ggattctggg
     9481 cgaaaaccgc gaaagataat gccaccctgg aacgatatcg ccgggcgctg aatattaaat
     9541 ttaaccaacc ggaacaagct gcgcggacct tatccggcgg caaccagcaa aaaatcctga
     9601 ttgccaaatg tctggaagct tcgccgcaag ttttgattgt cgatgagccg acgcgcggcg
     9661 tggatgtctc ggcgcgtaat gatatctacc aactgttgcg cagcatcgcc gcacaaaatg
     9721 tggctgtgct gcttatctcc tctgatctgg aagagatcga actgatggca gatcgcgtgt
     9781 atgtgatgca tcagggcgaa attgcccact ctgcactgac cggacgcgat attaatgtcg
     9841 agaccattat gcgcgttgcc ttcggagata gtcagcgtca ggaggcgtca tgctgaaatt
     9901 tattcagaac aaccgtgaaa tcacggcact gctggcggtg gtgctgctgt ttgtattacc
     9961 cggttttctc gaccgtcagt atttaagtgt gcaaacgctg accatggttt atagcagcgc
    10021 gcaaatcctg atcctgctgg caatgggcgc gacgctggta atgcttacgc gcaatattga
    10081 tgtttcagtg ggttcgatta ccggaatgtg cgcggtgctg ttggggatgt tactgaacgc
    10141 ggggtattca ctacctgttg cttgtgtcgc gactttactg cttggtttgc tcgcgggatt
    10201 tttcaacggt gtcctggtcg cgtggctaaa gatccctgcc attgttgcca cccttggcac
    10261 gttagggttg tacagaggca tcatgttgct gtggactggc ggtaaatgga ttgagggatt
    10321 acccgccgaa ctgaaacaac tctccgcccc gctgctgttt ggcgtttccg cgattggttg
    10381 gttgacgata attctggtgg catttatggc ctggctgctg gcaaagacgg cgtttggacg
    10441 cagtttttat gtcacgggcg ataatttaca gggcgctcgt caactgggcg ttcgtacgga
    10501 agccattcgc attgtggcat tttcgttgaa cggctgcatg gcggcactgg cgggaattgt
    10561 gtttgcttcg cagattggtt ttatccccaa ccagaccggt accgtgctgg agatgaaagc
    10621 aattgcagcc tgcgtgctgg gcggcattag tttgctcggc ggttccggcg cgatcatcgg
    10681 tgcggtactc ggcgcatggt tcctgacgca gatcgatagt gtactggtgc tgttgcgcat
    10741 tccggcatgg tggaatgatt ttatcgcggg catggttctg ctggcggtgc tggtgtttga
    10801 tggacgcctg cgttgtgcgc tggaacgtaa tctgcggcgg caaaaatatg cccgctttat
    10861 gatgccaccg ccatccgtta aacccgcttc gtcaggtaaa aaacgggagg ccgcgtaatg
    10921 cgtattcgct acggttggga actggctctt gccgcactgc tcgttattga gattgttgca
    10981 tttggtgcaa ttaacccgcg gatgttagat ctcaatatgt tgctgttcag caccagtgac
    11041 tttatctgca ttggcattgt cgccctaccg ctgacgatgg tgattgtcag tggcgggatc
    11101 gatatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt actgtttcaa
    11161 agtggtgtgc cgatgccgct ggcgattctt ctgaccttac tgctcggcgc attgtgcggg
    11221 ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat tacgcttggc
    11281 acgctgtatc tgtttgccgg aagcgccctg ctgctttccg ggatggccgg agcgacgggg
    11341 tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct ggatgtgctg
    11401 gggctccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg gctctggctg
    11461 cataaaaccc atgccggacg taacgtgttt ttaattgggc aaagcccgcg tgtggcggtt
    11521 tatagcgcga ttccagttaa ccgcaccttg tgtgcgctct atgccatgac ggggctggcg
    11581 tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc cgatctcggt
    11641 gcgtcgtttc tgatgcccgc catcaccgcc gtagtgctcg gcggtgccaa tatttatggt
    11701 ggttctggtt ccattatcgg caccgccata gcggttttat tagtgggata tttgcaacaa
    11761 ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg tgcgctactt
    11821 atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa agagtggctg
    11881 gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt catcgcttta
    11941 agaaaatcgc cttacttagc gcgcttggca ttgccgtaat ctctatgaat gtgcaggcgg
    12001 cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc agcggtggca
    12061 acggcgcaca acaagcgggt aaagagttgg gcgttgatgt gacctacgac gggccgacag
    12121 aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcag gggtataacg
    12181 ccattatcgt ttctgcggtt tcgcctgatg gcttgtgtcc ggcactgaaa cgcgccatgc
    12241 aacgtggtgt gagagtgctg acctgggact ctgatactaa accggagtgc cgctcttact
    12301 acattaatca gggaacgccc gcccagttgg gaggtatgtt ggtggatatg gcggcgcgtc
    12361 aggtgaataa agacaaagcc aaagtcgcgt ttttctactc aagccccacc gttacggacc
    12421 aaaaccagtg ggtgaaagaa gcgaaagcga aaatcgccaa agagcatcca ggctgggaaa
    12481 ttgtcactac gcagtttggc tataacgatg ccactaaatc gttacaaacc gcagaaggaa
    12541 tattaaaagc ctatagcgat ctcgacgcca ttatcgcccc cgatgccaat gccctgcccg
    12601 ctgccgcaca agccgcagaa aacctgaaaa atgacaaagt tgcgattgtc ggattcagta
    12661 cgccaaacgt gatgcgtcca tatgtggaac gcggcacggt gaaagaattt ggcctgtggg
    12721 atgtggttca gcaaggcaaa atatcagttt atgtcgcgga tgcattattg aaaaaaggat
    12781 caatgaaaac gggcgacaag ctggatatcc agggcgtagg tcaggtggaa gtctcgccaa
    12841 acagcgttca gggctatgac tacgaagctg atggtaatgg catcgtactg ttaccggagc
    12901 gcgtgatatt caacaaagag aatatcggca aatacgattt ctgatgtgca ttacttaacc
    12961 ggagtacgtt atggcagatt tagatgatat taaagatggt aaagattttc gtaccgatca
    13021 accgcaacaa aatatccctt ttaccctgaa aggttgcggt gcactggatt ggggaatgca
    13081 gtcacgctta tcgcggatat ttaatccgaa aacgggtaaa accgtgatgc tggcctttga
    13141 tcatggttat tttcaggggc cgactaccgg acttgaacgc attgatataa atatcgcccc
    13201 gctgtttgaa catgccgatg tattaatgtg tacgcgcggc attttgcgca gcgtagtccc
    13261 cccggcgacc aataagccgg tggtactgcg ggcgtcaggt gcgaactcta ttctggcgga
    13321 attaagtaat gaagccgtgg cgttatcgat ggatgatgcc gtgcgcctga acagttgcgc
    13381 ggtggcggcg caggtttata tcggcagcga atatgaacat cagtcgatca aaaatattat
    13441 tcagctggtc gatgccggaa tgaaagcggg aataccgacc atggccgtga ctggtgtggg
    13501 caaagatatg gtgcgcgatc agcgttattt ctcgctcgcg actcgaatcg ccgctgaaat
    13561 gggggcacag attatcaaaa cttattatgt cgataaaggt tttgaacgga ttgttgccgg
    13621 atgtccggta cccattgtta ttgctggcgg taaaaagtta ccggagcgcg aggcgcttga
    13681 aatgtgctgg caggctatcg atcagggcgc ttctggtgtg gatatggggc gtaatatttt
    13741 ccagtctgac catccggtgg cgatgatgaa agccgtacag gcggtggtac accataacga
    13801 aacggctgat cgggcatatg aactctatct gagtgaaaaa cagtaactgc ggatttaagg
    13861 agaagaatta tgcacgtcac actggttgaa attaacgttc atgaagataa ggttgacgag
    13921 tttatcgaag tttttcgcca gaaccacctg ggctctgtac aggaagaagg caatttgcgc
    13981 ttcgatgtct tacaggaccc ggaagtgaat tcgcgctttt atatctacga agcctataaa
    14041 gatgaagacg cagtggcatt ccataaaacc acgccccact acaaaacctg tgtcgcgaaa
    14101 ctggaatctt taatgaccgg gccgcgtaaa aaacgtctgt tcaatgggtt gatgccgtga
    14161 ggcgaattta tcagttttat ctacaattgg ggtaacgcgc tgacgggagt aaataaatgt
    14221 ctgactggaa cccctcttta tatctgcact ttgccgctga acgatcgcgt ccggcggtgg
    14281 agctgcttgc cagagtgccg ctggagaata tcaaatacgt tgccgatctt ggctgtggcc
    14341 caggtaacag caccaccctg ctgcatcaac gttggcctgc ggccaggata acaggcatcg
    14401 actcgtctcc ggcgatgatt gctgaagcgc gcagtacttt gccagactgc cagttcgtgg
    14461 aagcagacat ccgcaactgg caaccggaac aggcactcga tctgattttt gctaatgcct
    14521 cactgcaatg gctgcccgac cactacgaat tgttccctca tctggtttct ttacttaatt
    14581 cgcatggtgt gttggcagta cagatgccag ataactggct agagccaacc catgtgctca
    14641 tgcgcgaagt cgcctgggaa caaaattatc cggatcgcgg gcgtgagccg ctggctggcg
    14701 ttcatgctta ctacgatatt ctgagtgaag ccggatgtga ggtcgatatc tggcgaacca
    14761 cttactatca ccagatgccg tcgcaccagt cgattatcga ttgggtgacc gccactggat
    14821 tacgtccgtg gttacaggat ctgaccgaga gcgaacagca gcattttctt acgcgctacc
    14881 atcagatgct ggaagagcaa tatcccctcc aggagaacgg acagatattg ctggcatttc
    14941 cgcgtctgtt tattgttgcc cgccgtacgg agtaattatc acgtcagctg gtaatggcga
    15001 tcgggaagaa ttttcgctgg aatttctgct tcatcgttca tctgtaacag gtcaatttca
    15061 atagcgttgc agatggcatc cagcggtaag tcattgtttt cagtaccgaa gggatcttcc
    15121 agttcttccg ccagacaatc cagcgaaata aaagtgtagg aaatcagcac agagataaaa
    15181 ggcgtcatgt aatgcaggtc cacgaccagc gcaaacggca gcatgataca aaacagatac
    15241 acggtgcgat gcaaaatcag ggtgtaggca aagggaattg gcgtataggc aatgcgctcg
    15301 catcctgcca ggaccactga aatatcatta agccgatcgt tgaggctaat aaacaggata
    15361 tctgaaagtt gtccattgcg tcgctgaacc gccaaccact cccccattat taacaagatg
    15421 cggttagctg gagagttaga agccagtaca cgctgaagat cttcagtctt gagataatga
    15481 gccagcactt ccgcctgtgg ctgtttgcgt aatgtcatgc gtaaacagtg ggcgaaggcg
    15541 atttgcagcc gggcaaactc ccttacactt gccgaatccg gcaatgtcgt ttttacctcg
    15601 cgcagtaacg accgcgaggc aatcatcaac tgcccccaaa gttttcgcgc ttcaacgtaa
    15661 cgagcgtaac cggcattatt acgaaaacca agaaaaatgg cgatggcgac accgagaatg
    15721 ctgaacggtg cgagggtgaa tttaatgccc aaatgcgtgt accagggcag catgaaaata
    15781 acagcgatag aaaagagaaa attgagtagt aagcgcgagg atatcttgga taatactgaa
    15841 ccgtgccaga caaaaatacg gcgcagccag tgttgttgtg gacgaacaat catggttatc
    15901 ttcaggcgtg gaaaagtcgc cctattaaac gtgattacga tcacattctc aagacactac
    15961 ttacaaatta cctacaagct tacaacatta acaggtgctg catgtggcac ctgttaatga
    16021 taaaggcgca taggatgttg taactaatat agtgaaataa gaaacccggt cgaaaccggg
    16081 ttcagagtat tagcacaacg gacgtacagc ttcgcgcatt ccttttttga gaatcgcatc
    16141 caggtcatta gccacctgct cgaccagacc aggcacttgc gtcaggtcct gctcccagtg
    16201 gtctttttct gccaacacaa tcgctaccag ttcctgggta ccgataacac ggtcacgatg
    16261 ctggctccag agttgctgat aacgttccag ccagtgtgca tcgtcctgta ccggatatgt
    16321 ttctccatta cgctcaccgc gataaaacgc aatcaatgcc gctaatgcga aagtcaggcg
    16381 cgccggaagt gtgccgtttg ccttctgccc tgccagcagc tgcggcagga tacgggtgcg
    16441 gaacttggtc ataccgttaa gcgcgataga cagcagctga tgcttgatgt acggattacg
    16501 gaaacgtccg gtgactgcac tggcgaaaga ttccagttca tcacgaggca aatccagtac
    16561 cggaataatc tcttcgtaga tggctttttc aacgaatgcg caaatttcag catcgttcat
    16621 cgcttcacct acggtatcca gtcctgcctg gaacgccacc ggcaccagcc cggtgtgcgc
    16681 accgttgagg attgccactt tgcgctcttt atacggttta atatcgtcaa caatcagcac
    16741 gttgagtgga tatttgtcca ggcgcagctc agtcgctaag gatttcggcc cctgaatcac
    16801 aaacaggtaa aagtgttcag cggtgtcgag gaaaccatcg tgataaccca gttcttcttc
    16861 cagtttagcc acttcatcgc gcggataacc ggtaacgata cggtccacca gcgtagaaca
    16921 gaagctgtta gcctgatcca gccattgaat aaatgcttct ggcagtgccc actcttgcgc
    16981 atagcgcaga accagttcac gcaacgcgtc gccattgtag tcgatcaact cacacgggat
    17041 gatgatccaa cctttatcca gcgcaccgtt gaaatggcta aaacgttcga acagcagacg
    17101 ggtcagtttt gccggataac ttaccgctgg cgcgtcatcg aatttatcgc ccgcatggta
    17161 gctgataccc gcttcggtgg tgttagagaa aacaaagcgc atttccgggt tgtgcgccag
    17221 tttcaggaat tcatcgtatt cactgtagac gctgatttca cgattaacag agcgaatcag
    17281 acgcgcgtcg ctgaccgctt cccccttctc attcaggcca cggataatgg tggtgtacag
    17341 accatcctgt gtgctcagtg acggtgggaa tgaagtttca atcggacgaa caacgaccac
    17401 gccagaattc agatcggtgt gctcattcag gagatcaatt tgccagtcaa caaaggcgcg
    17461 caggaagtta ccttcaccaa actgaatgat acgttctgga tactgtgcac cgggaaaatc
    17521 gcgacgattt agtgttttca caatgggttc ccttctgatt agtcatacaa cctgttttaa
    17581 ttggtacgac aggttagcaa actttaatac gccgaacccc tgttttgatc aactcctgat
    17641 gattaatgag cagttttatg agaaaagtgt ggcgcggatc atggtttaat cgaggaacta
    17701 cgccttttat tcgctcaata aagtggtaga acacattccg ttaaaatcgc tgtgaatgaa
    17761 taaagatgtc agacaacttc ctcaccgtaa cgcatagtgc tggtacggtt gcgcccatct
    17821 ttcttcgagc gatacaaaca tgtatcggct tcgaccatca atttattaaa gtcatcggtc
    17881 agcgttcggt aggatgcgca accactaccg acgccaatac ttaccgtgag ataaagcgtt
    17941 ttttgttgcc aggtgaacgg ttgcagttca acgcctttac ggattttttc cgccattagc
    18001 agaccatcga caggattcac cgatggcact gcaacagcaa attcttcgcc gcccattcgc
    18061 gccaccagcc ccttatcacc gacaatcttc tgaatatgct gggcaaacac gcttaacact
    18121 ttatcgccac attcatgtcc atagttatcg ttaatacttt tgaaatagtc gatatcaagc
    18181 agcatgacag tcagatgttg tgtctgtttc agcgatggac ttttcagcgc ctcataaaga
    18241 ccggaccgtg agtaaacctg agtcagaaaa tcaaagtcgg ctcgcagcgc aacttgcttc
    18301 attagcgaat tgatcgctgc cacgctaaaa gaaaccataa ttgggcatat cgccatcgtg
    18361 gcaataccga gacgtgcgga gaacatttgc ggaatggaga acggcgaacc gaccgagata
    18421 tcaatcaccg aatttgccac cagtacgatt tccaccgcac cggtgacaaa ggtcaacaga
    18481 catgttacct gcggcgtata gcgcactgca caccagatta atgcaggcag cgggaacgcc
    18541 agactccccg ctccgccaat gactaccgag gcaataaccg acacaataag cgcgatagcg
    18601 ggcatcatct gttctgcttt aaagcgcggc agcactccag gaattgccaa cgtcagcata
    18661 caaggcacga tcaacacgcc cgttgagaat tgctcactga accagtccgc aagcaaaggc
    18721 cagaaatcca gactatcaat actgaccgaa ccaatcgccc cgacaatagc gcataataat
    18781 gcggcaatca gacagtaatt aaatagccgt aaggcactta ctggctcata cttatttttt
    18841 cccagacgct tatcccgcgc gaccagtaag gcgacggtaa caataaacat catattggag
    18901 aaattgatag ccagtgaaac cagcccccat tcggtggtta tggcatcata aaccagcatc
    18961 gccacataac tgatcgcata gtaatgcagt cgattaagcc agacatagcg ggcaaacacc
    19021 cccgccatta cgccattcag cggccagaaa agagaaagtt cgtgaaccag ccgtaactct
    19081 gcaccaataa aatagaacag cgtggttagc acaaaaatag cgatgctatt acgtagcaga
    19141 tgaccttcct ggaacaaacg gaaagttgaa atgggctgta catgcattaa ttatctgtcc
    19201 atacaattgc gggtggcaat tatctcaacg caataagtaa attcttagaa aggatagcat
    19261 gttcgctgac aacggcggca actaaagata tcctgggaat gctattttat cgatttccgt
    19321 aattatcatg gctgattcat ctgtataacc agccagtggt agattaccgt cacaatataa
    19381 cgttgttgct cctcacttaa cgcttccccc tgaggaatgt tatgccactt agccagacaa
    19441 ccgcgacaac aggtcgcggt ggcgtgttga gcgataaaaa ccggatgccc gcgcatgggg
    19501 gtttgcttac cgtcgttagc cggtaatgcc ggagcaagac gtttagcgac aaaatccgcc
    19561 gcatgttgtt caatgactgg cgcgcctttc tccagacaat actgacgctc tttcacaccc
    19621 aaacgaaagc gagagcgaaa ttttgaacgg gataaacgcg cgaagagtgg atcgagagac
    19681 tgcattaata aactgaacgc cctaattgtt tcgtcaattg ttcaaggacg gcaatgcccg
    19741 caagcgagtt accggcatca tccagttccg gactccagac tgcgatggcc atttcatgcg
    19801 gaacaatcgc cacaataccg ccaccaacgc cagattttgc cggtagtccc acccgccagg
    19861 caaactcccc cgcgttctgg tacataccgc tggtcgccat cagcgcgtta atttgccgcg
    19921 cctgcattgg cgtcaccaca ggttcatcaa tatgaatagc tttcccctga ttcgccagaa
    19981 agacaaacgt ccgggccagc tctatacagc tcattttcag agcgcagtaa tgaaagtagt
    20041 tttgcagaac ggttgtcacg tcatgatgga aattgccaaa cgacttcatc agccaggcga
    20101 tagccgcatt tcgcgcggaa tgttcaaatt cggaacgcgc taccaccgta tcgtaggaaa
    20161 tatcagacac accacttaag ccgcgcacga cttccagcat acgttgccga ggtgcgctta
    20221 atcgcccttg caacatatcg cagaccacca gcgcaccggc attaataaac ggattacgcg
    20281 gaataccctg ctccatttcc agttgcacta aagaattgaa cggtgatcca gacggatctt
    20341 tgccgacgcg ttgccagatt tcctcttcgg agtaatgacg catggcgaca acgagactca
    20401 gcactttgga aatagactga atggaaaaac gttcttgcgc gtctccggcc tgaaaaagct
    20461 gtccgtcaac ggtacagata gcaatcccca ttcgggaacc gtctaccgta gccagcgccg
    20521 gaatataatc cgcgacttta ccctgaccaa tgagcggccg cacttgccgc aagatgtttt
    20581 ctaaaattgc attatccatg gcgactgcca ctttctactc ctggaccgca ggtctgaaaa
    20641 gacctgcgag tatatcagag ctgaatatgt agcgtcagat ccggtctttc cacaccgtct
    20701 ggatattaca gaattcgtgt aagccaaaat gggaaagctc acgcccaaag ccactcttct
    20761 tcacgccacc aaaagcgact cgcgcgtcgc tggcacagta accattgata aacaccccac
    20821 cgcattccag acgtgccgcc atctgtctgg cccgggtttc gtcggtggtg aaaatggtcg
    20881 ctgaaaggcc gaactcgcta tcattagcca gttccagtgc atgttctgca tctttcgcaa
    20941 tggtgattgc cgcaacaggg ccaaacattt cttcccgaaa cgcggtcatt tctggggtaa
    21001 cgttcgccag aaccgttggc ggatagtagt tacctgcccc agccatcttt tccccgccca
    21061 gtaacaaacg cgcaccctgc gccagggttt tttccacctg atgatgcagc tcatcacgta
    21121 aatcaaaacg agccattggt ccgagagcgt tctcttcgtc acggggatcg cccattttca
    21181 aggctgccgc agctgccaca aaacgttcgg taaatgccga agcaattccc tcttcgataa
    21241 taaagcgttt tgccgccgca caaacctgtc cggtattctg ataacgtccg gctaccgccg
    21301 ctttaaccgc cagttccaga tcggcatcgt taagcacaat aaatggatcc gaaccgccca
    21361 gttccagtac gcattttttc agtgccgctc cagcctgtgc gccaatagcc gctcccgcac
    21421 gaacacttcc ggtcactgtg acagcagcaa tgcgcgaatc tttaatcatc tgactgacac
    21481 cgttgttgtc agcattcagc cagccatata cgccttgcgg gatacccgca tctttaaaca
    21541 cctgggcaat gagctgtgca cagcccatca cattcggcgc atgtttaagt aagtagccgt
    21601 tacctgcaag aatgatggga accgcgccac gcatcacctg ccataacgga aaattccacg
    21661 gcataatcgc cagaattgtc cccaacggtc gatactcaat aactgcctgc tgattttcca
    21721 ccagcgtagg ttccgccttc agcattgccg gaccatgttc tgcataccag tcacacaaat
    21781 tcgccgattt cgccacttca gcgcgcgcct ggttgattgg tttgcccatt tcacgggtga
    21841 tcatttgcgc catttcttcg ctacggacac gcagagcctt accgatatca cgcagttttt
    21901 cagcacgata atctatattt gtctcgcgcc agtcgcgaaa gcctgctgcc gccagctgaa
    21961 gtgcgttttc gatatcatca gcgccagccc acggcagcac agaaagttgt tcaccggtgg
    22021 caggatttat cgaaattgca tgagttgccg gagtaatggt catcggggta tctcctttat
    22081 gagttatggt aaaaagatac gcagatttac tcttgcttta aaatgaataa tattaagcca
    22141 cttattcacg aatcgagaat attatggatc tgacccaact ggagatgttc aacgcggttg
    22201 ccgaagctgg cagcattacg caggctgcaa caaaagtgca tcgcgtgccg tcgaatctca
    22261 ctacccgtct acgccagctg gaaacagaac tgggggttga tctgtttatt cgcgagaatc
    22321 agcgtttacg tctctcgcct gccgggcata actttttacg ctatagccaa caaattctcg
    22381 cgttagtgga tgaagcgcgg agcgttgtcg ctggcgatga gccgcaaggt ttattttctc
    22441 ttggttcgct ggaaagcacc gcagcagtgc gcattccggc catgctggcg gaatttaacc
    22501 gtcgttatcc caaaattcag ttttcacttt ccaccggccc ttccggcact atgctggaag
    22561 gggtgctgga gggaaaactg aatgcggcgt ttattgatgg gcccattaac catactgcca
    22621 ttgacgggat accggtgtac cgcgaggagc tgatgattgt cacgccacaa ggacatgcgc
    22681 cagtaacccg tgccagtcag gttaatggca gtaacattta tgccttccgc gccaattgtt
    22741 cgtatcgtcg ccacttcgag agctggtttc atgctgacgg tgccgctccg ggaactattc
    22801 acgagatgga gtcgtatcac gggatgttgg cctgtgtggt cgcaggagca ggcattgcgc
    22861 ttattccacg ctcaatgctg gaaagtatgc cggggcatca ccaggttgaa gcgtggccgt
    22921 tagctgagca atggcgttgg ttaacaacct ggctggtctg gcgtcgcggt gcgaaaacgc
    22981 gtccgcttga ggcatttatt caactactgg atgtgtctga ctcggcaaaa cagagttagt
    23041 aatgagctat ttttgatagt tcttgcggct aatatgctct atatagtgat gctccgatga
    23101 cttatgacta tatggggcaa atatggttac gccagtaagc atcagcaatt tcatctcact
    23161 tcctgatgat tttcccgttc gtaatattgc acctcaggta aaagaagaac aggccccctt
    23221 cctgatcgtc attaaaatgt gatgttaaca actctctgat gcgtgacaga acaaccgtca
    23281 gagagatgga aatgcgatcc agatcacaaa tgcattgtat tcacatcatt aaccgtttta
    23341 agatcatttc atcacttttt cgcaactcac ccgataatct gttatgacaa caaacactgt
    23401 ttcccgcaaa gtggcgtggc tacgggtcgt tacgctggca gtcgccgcct tcatcttcaa
    23461 caccaccgaa tttgtcccag ttggcctgct ctctgacatt gcgcagagtt ttcacatgca
    23521 aaccgctcag gtcggcatta tgttgaccat ttacgcatgg gtagtagcgc taatgtcatt
    23581 gccttttatg ttaatgacca gccaggttga acggcgcaaa ttactgatct gcctgtttgt
    23641 ggtgtttatt gccagccacg tactgtcgtt tttatcgtgg agttttaccg ttctggtgat
    23701 cagtcgcatt ggtgtggctt ttgcacatgc gattttctgg tcgattacgg cgtctctggc
    23761 gatccgtatg gctccggccg ggaagcgagc acaggcattg agtttaattg ccaccggtac
    23821 tgcactggcg atggtcttag gtttacctct cgggcgcatt gtggggcagt atttcggttg
    23881 gcgaatgacc ttcttcgcga ttggtatcgg ggagcttatc acccttttgt gcctgattaa
    23941 gttacttccc ttactgccca gtgagcattc cggttcgctg aaaagcctcc cgctattatt
    24001 ccgccgccca gcattgatga gcatttattt gttaactgtg gtggttgtca ccgcccatta
    24061 cacggcatac agctatatcg agccttttgt gcaaaacatt gcgggattca gcgccaactt
    24121 tgccacggca ttactgttat tactcggtgg tgcgggcatt attggcagcg tgattttcgg
    24181 taaactgggt aatcagtatg cgtctgcgtt ggtaagtacg gcgattgcgc tgttgctggt
    24241 gtgcctggca ctgctgctac ctgcggcgaa cagtgaaata cacctcgggg tgctgagtat
    24301 tttctggggg atagcgatga tgatcatcgg gcttggtatg caggttaaag tgctggcgct
    24361 ggcaccagat gctaccgacg tcgcgatggc gctattctcc gggatattta atattggaat
    24421 cggcgcgggt gcgttggtag gtaatcaggt aagtctgcac tggtcaatgt cgatgattgg
    24481 ttatgtgggc gcggtgcctg cctttgccgc gttaatatgg tcaatcatca ttttccgccg
    24541 ctggccagtg acactcgaag aacagacgca atagttgaaa ggcccatttg ggcctttttt
    24601 agtggtacgt tttaatgatt tccaggatgc cgttaataat aaactgcacc cccatacata
    24661 ccagcaggaa tcccatcaga cgggagatcg cttcaatgcc ccccttaccc accagccgca
    24721 taattgcgcc agagctgcgt agacttcccc acaaaataac cgccaccagg aaaaagatca
    24781 gcggcggcgc aaccatcagt acccaatcag caaaggttga actctgacgc actgtggacg
    24841 ccgagctaat aatcatcgct atggttcccg gaccggcggt acttggcatt gccagtggta
    24901 caaaagcaat attggcgctg ggttcatctt ccagctcttc cgacttgctt ttcgcttctg
    24961 gtgaatcaat cgctttctgt tgcggaaaga gcatccgaaa accgataaac gcgacgatta
    25021 agccgcctgc aattcgcaga ccgggaatcg aaatgccaaa tgtatccatc accagttgcc
    25081 cggcgtaata cgccaccatc atgatggcaa agacgtacac cgaggccatc aacgactgac
    25141 gattacgttc ggcactgttc atgttgcctg ccaggccaag aaataacgcg acagttgtta
    25201 atgggttagc taacggtagt aacaccacca gccccaggcc aattgcttta aataaatcta
    25261 acattggtgg ttgttatcct gtgtatctgg tttatcagcg aaaagtataa ggggtaaaca
    25321 acgataaagt gtcactcttt agctaacctt gcatcgcatt gaccaaaact tgaaccgatt
    25381 tagcaaaacg tggcatcggt caattcattc atttgactta tacttgcctg ggcaatatta
    25441 tcccttgcaa ctaattactt gccagggcaa ctaatgtgaa aagtaccagc gatctgttca
    25501 atgaaattat tccattgggt cgcttaatcc atatggttaa tcagaagaaa gatcgcctgc
    25561 ttaacgagta tctgtctccg ctggatatta ccgcggcaca gtttaaggtg ctctgctcta
    25621 tccgctgcgc ggcgtgtatt actccggttg aactgaaaaa ggtattgtcg gtcgacctgg
    25681 gggcgctgac ccgtatgctg gatcgcctgg tctgtaaagg ctgggtggaa aggttgccga
    25741 acccgaatga caagcgcggc gtactggtaa aacttaccac cagcggcgcg gcaatatgtg
    25801 aacaatgcca tcaattagtt ggccaggacc tgcatcaaga attaacaaaa aacctgacgg
    25861 cggacgaagt ggcaacactt gagcatttgc ttaagaaagt cctgccgtaa acaaaaaaag
    25921 aggtatgacg atgtccagac gcaatactga cgctattacc attcatagca ttttggactg
    25981 gatcgaggac aacctggaat cgccactgtc actggagaaa gtgtcagagc gttcgggtta
    26041 ctccaaatgg cacctgcaac ggatgtttaa aaaagaaacc ggtcattcat taggccaata
    26101 catccgcagc cgtaagatga cggaaatcgc gcaaaagctg aaggaaagta acgagccgat
    26161 actctatctg gcagaacgat atggcttcga gtcgcaacaa actctgaccc gaaccttcaa
    26221 aaattacttt gatgttccgc cacataaata ccggatgacc aatatgcagg gtgaatcgcg
    26281 ctttttacat ccattaaatc attacaacaa ctagttgata acgtgacaac gtcactgagg
    26341 caatcatgaa accactttta tccgcaatag caactgcgct tattctcttt tctgcgcagg
    26401 gcgttgcgga acaaaccacg cagccggttg ttacttcctg tggcaatgtc gtggttgttc
    26461 ccacatcgca ggaacaacca ccgtttgatt taaatcacat gggtactggc agtgataagt
    26521 cggatgcgct cggcgtgccc tattataatc aacacgctat gtagtttgtt ctggccccga
    26581 catatcgggg cttattaact tcccactttt accgctttac gccaccgcaa gccaaataca
    26641 ttgatataca gcccggtcat aatgagcacc gcaccgaaaa attgtagacc cgttaagcgt
    26701 tcatccaata atagtgccgc acttgccagt cctactacgg gcaccagtaa cgataacggt
    26761 gcaacccgcc aggtttcata gcgtcccagt aacgtccccc agatcccata accaacaatt
    26821 gtcgccacaa acgccagata catcagagac aagatggtgg tcatatcgat agtaaccaga
    26881 ctgtgaatca tggttgcgga accatcgaga atcagcgagg caacaaagaa gggaatgatt
    26941 gggattaatg cgctccagat taccagcgac atcaccgccg ggcgcgttga gtgcgacatg
    27001 atctttttat tgaagatgtt gccacacgcc caactaaatg ctgccgccag ggtcaacata
    27061 aagccgagca tcgccacatg ctgaccgttc agactatctt cgattaacac cagtacgcca
    27121 aaaatcgcta aggcgatccc cgccaattgt ttgccatgca gtcgctcccc gaaagtaaac
    27181 gcgccaagca cgatagtaaa aaacgcctgt gcctgtaaca ccagcgaagc cagtccagca
    27241 ggcataccaa agttaatggc gcaaaacaga aaggcaaact gcgcaaaact gatggttaat
    27301 ccatacccca gcagcaaatt cagtggtact ttcggtcgag cgacaaaaaa gatggccgga
    27361 aaagcgacca gcataaagcg caaaccggcc agcatcagcg gtggcatgtt atgaagcccc
    27421 actttgatga ccacaaaatt cagcccccat acgaccacta ccagtagcgc caacacccca
    27481 tcttttcgcg acattatacc gcctctgaat ttcatctttt gtaagcaatc aacttagctg
    27541 aatttacttt tctttaacag tcgattcgtt agtcaccgtt tacgacggca ttaatgcgca
    27601 aataagtcgc tatacttcgg atttttgcca tgctatttct ttacatctct aaaacaaaac
    27661 ataacgaaac gcactgccgg acagacaaat gaacttatcc ctacgacgct ctaccagcgc
    27721 ccttcttgcc tcgtcgttgt tattaaccat tggacgcggc gctacgctgc cgtttatgac
    27781 catttacttg agtcgccagt acaacctgag tgtcgatcta atcggttatg cgatgacaat
    27841 tgcgctcact attggcgtcg tttttagcct cggttttggt atcctggcgg ataagttcga
    27901 caagaaacgc tatatgttac tggcaattac cgccttcgcc agcggtttta ttgccattcc
    27961 tttagtgaat aacgtgacgc tggtggtgct cttttttgcc ctcattaact gcgcctattc
    28021 cgtttttgct accgtgttga aagcctggtt tgccgacaat ctttcgtcca ccagcaaaac
    28081 gaaaatcttc tcaatcaact acaccatgct caacattggc tggaccatcg gtccgccgct
    28141 cggcacgttg ttggtgatgc agagcatcaa tctgcccttt tggctggcag ctatctgttc
    28201 cgcgtttccc atgcttttca ttcaaatttg ggtaaagcgc aacgagaaaa tcatcgccac
    28261 ggaaacaggc agtgcctggt cgccgaaagt tttattacaa gataaagcac tgttgtggtt
    28321 tacctgctct ggttttctgg cttcctttgt tagcggcgca tttgcctcgt gcatttcaca
    28381 atatgtgatg gtgattgctg atggtgattt tgccgaaaag gtggttgcgg ttgttcttcc
    28441 ggttaatgct gccatggtag ttacgttgca atattccgtg ggccgccgac ttaacccggc
    28501 taacatccgc gcgctgatga cagcaggcac cctctgtttc gtcattgggc tggtcggttt
    28561 tattttttcc ggcaacagcc tgctattgtg gggtatgtca gctgcggtat ttactgtcgg
    28621 tgaaatcatt tatgcgccgg gcgagtatat gttgattgac catattgcgc cgccaggaat
    28681 gaaagccagc tatttttccg ctcagtcttt aggctggctt ggtgcggcga ttaacccatt
    28741 agtgagtggc atagtgctta ccagtctgcc gcctttctcg ctgtttatca tcttagcgtt
    28801 ggtgatcatt gctgcgtggg tgctgatgtt aaaaggaatt cgtgcaagac cgtgggggca
    28861 gcccgcgctt tgttgattta agtcgaacac aataaaaatt taattcagcc ttcgtttagg
    28921 ttacctctgc taatatcttt ctcattgaga tgaaaattaa ggtaagcgag gaaacacacc
    28981 acaccataaa cggaggcaaa taatgctggg taatatgaat gtttttatgg ccgtactggg
    29041 aataatttta ttttctggtt ttctggccgc gtatttcagc cacaaatggg atgactaatg
    29101 aacggagata atccctcacc taaccggccc cttgttacaa ctgtgtacaa ggggcctgat
    29161 ttttatgacg gcgaaaaaaa accgccagta aaccggcggt gaatgcttgc atggatagat
    29221 ttgtgttttg cttttacgct aacaggcatt ttcctgcact gataacggat cgttgacaca
    29281 gtagcatcag ttttctcaat gaatgttaaa cggagcttaa actcggttaa tcacgttttg
    29341 ttcgtcaata aacatacagc gatttcttcc ggtttgctta ccctcataca ttgcccggtc
    29401 cgctcttcca atgaccacat ccagaggctc ttcaggaaat gcgcgactca cacctgctgt
    29461 cacggtaatg ttgatatgcc cttcagaatg tgtgatggca tggttatcga ctaactggca
    29521 aattctgaca cctgcacgac atgcttcttc atcattagcc gctttgacaa taatgataaa
    29581 ttcttcgcca ccgtagcgat aaaccgtttc gtaatcgcgc gtccaactgg ctaagtaagt
    29641 tgccagggtg cgtaatacta catcgccgat taaatgcccg taggtatcat taaccaattt
    29701 aaatcggtca atatccaaca acattaaata aagattcaga ggctcagcgt tgcgtaactg
    29761 atgatcaaag gattcatcaa gaacccgacg acccggcaat cccgtcaaaa catccatatt
    29821 gctacggatc gtcagcaaat aaattttgta atcggttaat gccgcagtaa aagaaagcaa
    29881 cccttcctga aaggcgtcga aatgcgcgtc ctgccagtga ttttcaacaa tagccagcat
    29941 taattcccga ccacagttat gcatatgttg atgggcagaa tccattagcc gaacgtaagg
    30001 taattcatcg ttatcgagtg gtcccagatg atcaatccac cgaccaaact ggcacagttc
    30061 ataagaatgg ttatccgtta tttctggctt actggcatct ctcgcgacca cgctgtgaaa
    30121 catacttacc agccactggt agtgggcatc gatagcctta ttgagattta acaagatggc
    30181 atcaatttcc gttgtcttct tgatcattgc cactcctttt tcacagttcc ttgtgcgcgc
    30241 tattctaacg agagaaaagc aaaattacgt caatattttc atagaaatcc gaagttatga
    30301 gtcatctctg agataacatt gtgatttaaa acaaaatcag cggataaaaa agtgtttaat
    30361 tctgtaaatt acctctgcat tatcgtaaat aaaaggatga caaatagcat aacccaatac
    30421 cctaatggcc cagtagttta ggccatcagg ctaatttatt tttatttctg caaatgagtg
    30481 acccgaacga cggccggcgc gcttttctta tccagactgc cgctaatgtt gatcatctgg
    30541 tccggctgaa cttctcgtcc atcaaagact gccgcaggaa taacaacatt aatttcacca
    30601 ctcttatcgc gaaaaacgta acggtcctct cctttgtgag aaatcaaatt accgcgtagt
    30661 gaaaccgaag cgccatcgtg catgtttttt gcgaaatcaa cggtcatttt ttttgcatca
    30721 tcggttccgc gatagccatc ttctattgca tgaggcggcg gtggcgctgc atcctgtttt
    30781 aatccgccct ggtcatctgc caacgcataa ggcatgacaa gaaaacttgc taatacaatg
    30841 gcctgaaatt tcatactgac t
//
LOCUS       DAZGHF010000048        30495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGHF010000048 DAZGHF010000000
VERSION     DAZGHF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.048"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..570
                     /locus_tag="ACHK3A_002290"
     CDS             <1..570
                     /locus_tag="ACHK3A_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO8740356.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIH
                     PIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            800..1183
                     /gene="secE"
                     /locus_tag="ACHK3A_002291"
     CDS             800..1183
                     /gene="secE"
                     /locus_tag="ACHK3A_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO8740357.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1185..1730
                     /gene="nusG"
                     /locus_tag="ACHK3A_002292"
     CDS             1185..1730
                     /gene="nusG"
                     /locus_tag="ACHK3A_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO8740358.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1889..2317
                     /gene="rplK"
                     /locus_tag="ACHK3A_002293"
     CDS             1889..2317
                     /gene="rplK"
                     /locus_tag="ACHK3A_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO8740359.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2321..3025
                     /gene="rplA"
                     /locus_tag="ACHK3A_002294"
     CDS             2321..3025
                     /gene="rplA"
                     /locus_tag="ACHK3A_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO8740360.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3438..3935
                     /gene="rplJ"
                     /locus_tag="ACHK3A_002295"
     CDS             3438..3935
                     /gene="rplJ"
                     /locus_tag="ACHK3A_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO8740361.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4002..4367
                     /gene="rplL"
                     /locus_tag="ACHK3A_002296"
     CDS             4002..4367
                     /gene="rplL"
                     /locus_tag="ACHK3A_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO8740362.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4687..8715
                     /gene="rpoB"
                     /locus_tag="ACHK3A_002297"
     CDS             4687..8715
                     /gene="rpoB"
                     /locus_tag="ACHK3A_002297"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO8740363.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8792..13015
                     /gene="rpoC"
                     /locus_tag="ACHK3A_002298"
     CDS             8792..13015
                     /gene="rpoC"
                     /locus_tag="ACHK3A_002298"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO8740364.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13255..14388)
                     /gene="thiH"
                     /locus_tag="ACHK3A_002299"
     CDS             complement(13255..14388)
                     /gene="thiH"
                     /locus_tag="ACHK3A_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO8740365.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINSKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQVKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSISFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(14385..15155)
                     /gene="thiG"
                     /locus_tag="ACHK3A_002300"
     CDS             complement(14385..15155)
                     /gene="thiG"
                     /locus_tag="ACHK3A_002300"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO8740366.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRACGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIITQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15157..15357)
                     /gene="thiS"
                     /locus_tag="ACHK3A_002301"
     CDS             complement(15157..15357)
                     /gene="thiS"
                     /locus_tag="ACHK3A_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO8740367.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQSGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15341..16096)
                     /gene="thiF"
                     /locus_tag="ACHK3A_002302"
     CDS             complement(15341..16096)
                     /gene="thiF"
                     /locus_tag="ACHK3A_002302"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO8740368.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRNLALRRASGCPVCGGSNADPV"
     gene            complement(16089..16724)
                     /gene="thiE"
                     /locus_tag="ACHK3A_002303"
     CDS             complement(16089..16724)
                     /gene="thiE"
                     /locus_tag="ACHK3A_002303"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO8740369.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16724..18619)
                     /gene="thiC"
                     /locus_tag="ACHK3A_002304"
     CDS             complement(16724..18619)
                     /gene="thiC"
                     /locus_tag="ACHK3A_002304"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO8740370.1"
                     /translation="MSVTKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGREHIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18700..18800)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18852..19328)
                     /gene="rsd"
                     /locus_tag="ACHK3A_002305"
     CDS             complement(18852..19328)
                     /gene="rsd"
                     /locus_tag="ACHK3A_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO8740371.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19423..20196
                     /gene="nudC"
                     /locus_tag="ACHK3A_002306"
     CDS             19423..20196
                     /gene="nudC"
                     /locus_tag="ACHK3A_002306"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO8740372.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20236..21300
                     /gene="hemE"
                     /locus_tag="ACHK3A_002307"
     CDS             20236..21300
                     /gene="hemE"
                     /locus_tag="ACHK3A_002307"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO8740373.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSAPYHL"
     gene            21310..21981
                     /gene="nfi"
                     /locus_tag="ACHK3A_002308"
     CDS             21310..21981
                     /gene="nfi"
                     /locus_tag="ACHK3A_002308"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO8740374.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22024..22614
                     /gene="yjaG"
                     /locus_tag="ACHK3A_002309"
     CDS             22024..22614
                     /gene="yjaG"
                     /locus_tag="ACHK3A_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO8740375.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22801..23073
                     /gene="hupA"
                     /locus_tag="ACHK3A_002310"
     CDS             22801..23073
                     /gene="hupA"
                     /locus_tag="ACHK3A_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO8740376.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23086..23781
                     /gene="yjaH"
                     /locus_tag="ACHK3A_002311"
     CDS             23086..23781
                     /gene="yjaH"
                     /locus_tag="ACHK3A_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO8740377.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23783..24202)
                     /gene="zraP"
                     /locus_tag="ACHK3A_002312"
     CDS             complement(23783..24202)
                     /gene="zraP"
                     /locus_tag="ACHK3A_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO8740378.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMDHW"
     gene            24440..25816
                     /gene="zraS"
                     /locus_tag="ACHK3A_002313"
     CDS             24440..25816
                     /gene="zraS"
                     /locus_tag="ACHK3A_002313"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO8740379.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGTGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQVGKGATFTLWLPVT
                     ITRKDPQG"
     gene            25813..27138
                     /gene="zraR"
                     /locus_tag="ACHK3A_002314"
     CDS             25813..27138
                     /gene="zraR"
                     /locus_tag="ACHK3A_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO8740380.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENTVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27135..28424)
                     /gene="purD"
                     /locus_tag="ACHK3A_002315"
     CDS             complement(27135..28424)
                     /gene="purD"
                     /locus_tag="ACHK3A_002315"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO8740381.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28436..30025)
                     /gene="purH"
                     /locus_tag="ACHK3A_002316"
     CDS             complement(28436..30025)
                     /gene="purH"
                     /locus_tag="ACHK3A_002316"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO8740382.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLLLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAISNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7141 a         7904 c         8415 g         7035 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacattct gtccaaagat gaaggcggtc gtcatactcc gttcttcaaa
      361 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
      421 gaaggtgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      481 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc
      541 gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact
      601 aaaagggcat catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt
      661 gattttcttt gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa
      721 gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaat
      781 acttctgaca ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct
      841 ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg gtagcgattg tcggcaacta
      901 cctttatcgc gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc
      961 agcgggtggt gtcgcgctgt taacgacaaa aggtaaagca accgttgctt ttgcccgtga
     1021 agcgcgtacc gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac
     1081 gctgattgtg gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat
     1141 tctggttcgc ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta
     1201 aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc
     1261 tgcgtgagca tatcaaatta cacaacatgg aagagttgtt tggtgaagtc atggtaccaa
     1321 ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc
     1381 ctggctacgt cctcgttcag atggtgatga acgacgcgag ctggcacctg gtgcgcagcg
     1441 taccgcgtgt gatgggcttc atcggcggta cttccgatcg tcctgcgcca atcagcgata
     1501 aagaagtcga tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa
     1561 cgttgtttga accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg
     1621 gcgttgttga agaagttgat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg
     1681 gtcgtgcgac ccctgtagag ctggacttca gccaggttga aaaagcttaa cccagcgatc
     1741 aaaaaagcgg cgatttaatc gttgcacaag gcgcgagatt ggaatacaat ttcgcgcctt
     1801 ttgtttttat gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg
     1861 cgttattacc caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct
     1921 gcaggttgca gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca
     1981 gggcgtaaac atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa
     2041 aggtctgccg attccggtag taatcactgt ttacgctgac cgttctttca ctttcgttac
     2101 caagactccg ccggcagcag ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg
     2161 taagccgaac aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca
     2221 gaccaaagct gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg
     2281 tactgcacgt tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg
     2341 catgcgtgtt atccgagaga aagttgatgc aaccaaacag tacgacatca acgaagctat
     2401 cgctctgctg aaagaactgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa
     2461 cctcggcatc gacgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca
     2521 cggtactggc cgttccgttc gcgtagccgt atttacccag ggtgcaaacg ctgaagctgc
     2581 taaagctgca ggtgctgaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg
     2641 cgaaatgaac tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct
     2701 gggccaggtt ctgggtccgc gcggcctgat gccaaacccg aaagtgggta ctgtaacgcc
     2761 gaacgttgct gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa
     2821 aaacggcatc atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga
     2881 aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt
     2941 gtacatcaag aaagttagca tctccaccac catgggtgca ggtgttgcag ttgaccaggc
     3001 tggcctgagc gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct
     3061 tacccccacg tataatgctt aatgcagacg tatatccgag atattcgggt tgtggcaagg
     3121 cggtaactga gtgagtcgcc aggagcatag ttaactatgt gactggtgcg aacgaaggaa
     3181 gccaacgccg ccacaagctg aatagcgacg gatagaaaag atttgttcgt tggagcctgg
     3241 cctatccagg cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta
     3301 gacggtgaca gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa
     3361 ttaagacgct ctctccgttt ggaggagtga agtgagttcc agagattttc tctggcaaac
     3421 atccaggagc aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag
     3481 tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg
     3541 tagataaaat gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg
     3601 ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg
     3661 cgtttgttgg tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc
     3721 tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag
     3781 gtgagctgat cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag
     3841 caattgcacg cctgatggca accatgaaag aagcttcggc tggcaaactg gttcgtactc
     3901 tggctgctgt acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc
     3961 gcttacgtat aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc
     4021 aaatcattga agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa
     4081 tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc tgtagctgct ggcccggttg
     4141 aagctgctga agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag
     4201 ttgctgttat caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc
     4261 tggtagaatc tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac
     4321 tgaaaaaagc tctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg
     4381 gttgcagcct gagaaatcag gctgatggct ggtgactttt tagtcaccag cctttttgcg
     4441 ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt
     4501 ttctatcgac gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa
     4561 tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa
     4621 tagtgttgca caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg
     4681 aaccctatgg tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt
     4741 ccacaagttc tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt
     4801 atcgagcaag atcctgaagg gcagtatggt ctggaagctg ctttccgttc cgtattcccg
     4861 attcagagct acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg
     4921 gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt
     4981 aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa
     5041 gaacaagaag tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc
     5101 aacggtactg agcgtgttat cgtttcccag ctgcaccgta gtccgggcgt cttctttgac
     5161 tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg catcatccct
     5221 taccgtggtt cctggcttga cttcgaattc gatccgaagg acaacctgtt cgtacgtatc
     5281 gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgcg ccctgaacta caccacagag
     5341 cagatcctcg acctgttctt tgaaaaagtt atctttgaaa tccgtgataa caagctgcag
     5401 atggaactgg tgccggaacg cctgcgtggt gaaaccgcat cttttgacat cgaagctaac
     5461 ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc gccacattcg ccagctggaa
     5521 aaagacgacg tcaaactgat cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct
     5581 aaagactata ttgatgagtc taccggcgag ctgatctgtg ctgcgaacat ggagctgagc
     5641 ctggatctgc tggctaagct gagccagtct ggtcacaagc gtatcgaaac gctgttcacc
     5701 aacgatctgg atcacggccc atatatctct gaaaccttac gtgtcgaccc aactaacgac
     5761 cgtctgagcg cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt
     5821 gaagcagctg aaagcctgtt cgagaacctg ttcttctccg aagaccgtta tgacttgtct
     5881 gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt
     5941 attctgagca aagacgacat cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt
     6001 aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc cgttggcgaa
     6061 atggcggaaa accagttccg cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt
     6121 ctgtctctgg gcgatctgga taccctgatg cctcaggata tgatcaacgc caagccgatt
     6181 tccgcagcag tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac
     6241 aacccgctgt ctgagattac gcacaaacgt cgtatctccg cactcggccc aggcggtctg
     6301 acccgtgaac gtgcaggctt cgaagttcga gacgtacacc cgactcacta cggtcgcgta
     6361 tgtccaatag aaacccctga aggtccgaac atcggtctga tcaactctct gtccgtgtac
     6421 gcacagacta acgaatacgg cttccttgag actccgtatc gtaaagtgac cgacggtgtt
     6481 gtaactgacg aaattcacta cctatctgct atcgaagaag gcaactacgt tatcgcccag
     6541 gcgaactcca acctggatga agaaggccac ttcgtagaag acctggtaac ctgccgtagc
     6601 aaaggcgaat ccagcttgtt cagccgcgac caggttgact acatggacgt atccacccag
     6661 caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt
     6721 gcattgatgg gtgcgaacat gcaacgtcag gccgttccga ctctgcgcgc tgacaagccg
     6781 ctggttggta ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggtagct
     6841 aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa
     6901 gacgagatgt atccgggtga agcaggtatc gatatctaca acctgaccaa atacacccgt
     6961 tctaaccaga acacctgtat caaccagatg ccgtgtgtgt ctctgggtga accggttgaa
     7021 cgtggcgacg tgctggcaga cggtccgtcc accgacctcg gtgaactggc gcttggtcag
     7081 aacatgcgcg tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta
     7141 tccgagcgtg ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt
     7201 gtgtcccgtg acaccaagct ggggccagaa gagatcaccg ctgacatccc gaacgtgggt
     7261 gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt acattggtgc ggaagtgacc
     7321 ggtggcgaca ttctggttgg taaggttacg ccgaaaggtg aaactcagct gaccccagaa
     7381 gaaaaactgc tgcgtgcgat cttcggtgag aaagcgtctg acgttaaaga ctcttctctg
     7441 cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc
     7501 gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa
     7561 gacctgtctg aagaactgca gatcctcgaa gcgggtctgt tcagccgtat ccgtgctgtg
     7621 ctggtagccg gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg
     7681 gagctgggcc tgaccgacga agagaaacaa aatcagctgg aacagctggc tgagcagtat
     7741 gacgaactga aacacgagtt cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag
     7801 ggcgacgatc tggcaccggg cgtgctgaag attgttaagg tatatctggc ggttaaacgc
     7861 cgtatccagc ctggtgacaa gatggcaggt cgtcatggta acaagggtgt aatttctaag
     7921 atcaacccga tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg
     7981 aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt
     8041 atggctgcga aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaagaagtc
     8101 gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa
     8161 gttgacctga gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa
     8221 ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg
     8281 ctgaaacttg gcgacctgcc gacttctggt cagatccgcc tgtacgacgg ccgcactggt
     8341 gaacagttcg agcgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg
     8401 gtcgacgaca agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg
     8461 ctgggtggta aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg
     8521 gaagcatacg gcgcagcata caccctgcag gaaatgctca ccgttaagtc tgatgacgtg
     8581 aacggtcgta ctaagatgta taaaaacatc gtggacggca accatcagat ggagccgggc
     8641 atgccagaat ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa
     8701 ctggaagacg agtaattctc gctcaaacag gtcactgctg tcggggtaaa acccggcagc
     8761 ggattgtgct aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc
     8821 gcagactaaa accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat
     8881 ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa
     8941 accagaacgt gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg
     9001 cctgtgcggt aagtacaagc gcctgaaaca ccgtggtgtc atctgtgaga agtgcggcgt
     9061 tgaagtgacc cagactaaag tacgccgtga gcgtatgggc cacatcgaac tggcttcccc
     9121 gactgcgcac atctggttcc tgaaatcgct gccgtcccgt atcggcctgc tgctcgatat
     9181 gccgctgcgc gatatcgaac gcgtactgta cttcgaatcc tatgtggtta ttgaaggcgg
     9241 tatgaccaac ctggaacgcc agcagatcct gactgaagag cagtatctgg acgcgctgga
     9301 agagttcggt gacgaattcg acgcgaaaat gggtgcggaa gcaatccagg ccctgctgaa
     9361 gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc
     9421 cgaaaccaag cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc
     9481 tggtaacaaa ccagagtgga tgatcctgac cgttctgccg gtactgccgc cagatctgcg
     9541 tccgctggtt ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg
     9601 tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat
     9661 catcgtacgt aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg
     9721 tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgacat
     9781 gatcaaaggt aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc
     9841 cggtcgttct gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa
     9901 gaaaatggcg ctggagctgt tcaaaccgtt catctacggc aagctggaac tgcgtggtct
     9961 tgctaccacc attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga
    10021 tatcctggac gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca
    10081 ccgtctgggt atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca
    10141 cccgctggtt tgtgcggcat ataacgccga cttcgatggt gaccagatgg cagttcacgt
    10201 accgctgacg ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat
    10261 cctgtccccg gcgaacggcg aaccaatcat cgttccgtct caggacgttg tactgggtct
    10321 gtactacatg acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc
    10381 gaaagaagca gaacgtctgt atcgctctgg tctggcctct ctgcatgcgc gcgttaaagt
    10441 gcgtatcacc gagtatgaaa aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa
    10501 agacacgact gttggccgtg ccattctgtg gatgattgta ccgaaaggtc tgccttactc
    10561 catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg
    10621 cattctcggt ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggctttgc
    10681 atatgcagcg cgttctggtg catctgttgg tatcgatgac atggtcatcc cggagaagaa
    10741 acacgaaatc atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc
    10801 tggtctggta actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa
    10861 cgatcgtgta tccaaagcga tgatggataa cctgcaaact gaaaccgtta ttaaccgtga
    10921 cggtcaggaa gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc
    10981 gcgtggttct gcggcacaga ttcgtcagct tgctggtatg cgtggtctga tggcgaagcc
    11041 ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact
    11101 ccagtacttc atctccaccc acggtgctcg taaaggtctg gcggataccg cactgaaaac
    11161 ggcgaactcc ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac
    11221 cgaagacgat tgtggtactc atgaaggtat catgatgact ccggttatcg aaggtggtga
    11281 cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa
    11341 gccgggtact gctgatatcc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga
    11401 cctgctggaa gagaactctg tcgacgcggt taaagtacgt tctgttgtat cttgtgacac
    11461 cgactttggt gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa
    11521 caaaggtgaa gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct
    11581 gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt gcggctgctg aatccagcat
    11641 ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag
    11701 cggtaaactg gttatcactt cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg
    11761 taccaaagaa agctacaaag taccttacgg tgcggtactg gcaaaaggcg atggcgaaca
    11821 ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga
    11881 agtaagcggt tttgtacgct ttactgacat gatcgacggt cagaccatta cgcgtcagac
    11941 cgacgaattg accggtctgt cttcgctggt ggttctggat tccgcagaac gtaccgcagg
    12001 tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg atgtactgat
    12061 cccgggtacc gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga
    12121 agatggcgta cagatcagct ctggtgacac cctggcgcgt attccgcagg aatctggcgg
    12181 taccaaggac atcaccggtg gtctgccgcg cgttgcggat ctgttcgaag cacgtcgtcc
    12241 gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa
    12301 aggtaaacgt cgtctggtta tcactccggt agacggtagc gatccgtacg aagagatgat
    12361 tccaaaatgg cgtcagctca atgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat
    12421 ttccgatggt ccggaagcgc cgcacgacat tctgcgtctg cgtggtgttc atgctgttac
    12481 tcgttacatc gttaacgaag tacaagacgt ataccgtctg cagggcgtta agattaacga
    12541 taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg
    12601 cagctccgac ttcctggaag gtgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg
    12661 cgaactggaa gcgaacggca aagtgggtgc aacttactcc cgcgatctgc tgggtatcac
    12721 caaagcgtct ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg
    12781 cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa
    12841 cgttatcgtg ggtcgtctga tcccggcagg taccggttac gcgtaccacc aggatcgtat
    12901 gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc
    12961 tgccagcctg gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt
    13021 aatccgcaaa taacgtaaaa actcgcttcg gcgggttttt ttatgggtgt tattcagagg
    13081 gcaattcttc acggaatttt taagaaaggc agggcgaaac gaggaagaag cttttcgccc
    13141 tggacaacat tcctgctgac gggactacca taaaaaaaca caacgctttc aacaaccttg
    13201 cgtttttcat cttccgtagc ggctcggaat aaatttacgt tgttacattc tgtctcatgg
    13261 cctttgcgag gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg
    13321 agcagttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc
    13381 cagctcgggg tgattgtcgg catagccacc tggctgcgtt ttcgaaaaag cgctgacgtt
    13441 attaattgcc agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga
    13501 cagttcaatc tccggtgcaa gcagccggaa ggcgcagatg gtttgcacta actggcgttc
    13561 atccataatc gacgcaggct caatgccgcc agtacatgga cgcaggcgtg ggaaggagat
    13621 agagtagcgg ctttgccagt aatgctgttg cagccatagc aaatgttctg caaccatata
    13681 gcagtcaacg cgccagctgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat
    13741 ccccgcacgg cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttacc
    13801 tttcagatga tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa ccatcacgcc
    13861 atccagacct agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat
    13921 ctgtagtgaa gagaactgtt cacgcagggc agggagatga cgacgaaagt aatccatccc
    13981 cactttcacc tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat
    14041 agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattgct
    14101 catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac
    14161 gtagaagctg acgacgttgc caaatcgctg acgggtcaga cgctgcgccc gttgggccag
    14221 tggttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt cgcgggtgag
    14281 ttgcgaggca tttagcgccc gctctacgtc agcagccgtt ttgctgttga tacgcaggcg
    14341 gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc tgatgcctcc
    14401 agaaatccgg tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc cggtccggac
    14461 tgacgtgcca gcaggcctgc ttctactgcc agacgaaatg ccttcgccat gttgacggga
    14521 tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc
    14581 tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa ccaccggaac cgtggcttgc
    14641 tgggtaataa tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg
    14701 ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg
    14761 gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg
    14821 gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa ttttaaccag
    14881 tttgtgccta acgcttcacg agccagatgg gcggcgaaaa tggcttcttc cgctgttttc
    14941 gccccggatg tatttggcag cagggtcaca cccgccgcga taagcggttc gaggatagcg
    15001 tcgttgtgct ggcgcaagtt gacgcgtttc atcgccagtg tcaccagttg gctgccgcaa
    15061 gcgcggatcg cctccatcat cagttgtgaa gaggcgaatt tccctgtgcc ggtaaacaga
    15121 tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa
    15181 aagcaggatc tggtcgccat cctgcacgat atgttgcgcc cactgctcac gcgggacgat
    15241 ttgctgatta atcgccagag ccgcgcccga ttgtcgttgg tcgagttgct ccagtagttc
    15301 gtgaaccgtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc
    15361 ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc caggttgcgc cactggctcg
    15421 atttaccgtc gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa
    15481 tggcttccag tgcctgtaaa gtgcccataa ccccgaccac cgggccaacc acgcccgccg
    15541 tgcggcagtt gcgttcaggc tcctggttat ctggccacag gcagcggtaa cacccctgct
    15601 cccagggcgg cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa
    15661 gcggcgtgtt gagtgccacg caggcggcat taatctcctg acgagtcgcc atattgtcgg
    15721 tacagtcgag caccacatcg gcccgtgcta ccgcatcttt taacgcctca cccgttaacc
    15781 gttgttgtaa tgctgtcagt tgaatatcgg gattcaactg tgtcagtcgc tgatggctga
    15841 cctgcgattt cgggcgatcg atatcttcag tggtaaagag gatttgtcgt tgcagattgc
    15901 ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg cccgccaggt
    15961 atagcgcagc aggtgtaccc agcccgccaa gaccgataat cagcacctgg ctgtcgagca
    16021 gtttttgctg cccgtcaaga gcgatatcgt cgagcaggat ttggcggcta taacgcataa
    16081 agtcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc
    16141 cagtctgcgg cttgagtaat ggcgcttacg acggcgatgc tgccgacacc cgttgctatc
    16201 accgcaggcg cgcgcgccag actgataccg ccaatcgcca cggtgggata atccgccagg
    16261 cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt
    16321 tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg
    16381 atctccatat cgtcatgggt cgaaacgccc agtcgcagac ctgccgcgcg gatggcgctg
    16441 agatcggtgg cttgcaaatc ttcctgcccc agatggacgc catatgcctg atgcttgatc
    16501 gccagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt
    16561 gccgccacga catcggcttc cacctcttca tcgcgtcgat ctttgatgcg tagctggaga
    16621 gtacgtacgc ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac
    16681 agtcctaaac gaaagggtac aggaggaaaa tctggctgat acatcacgct tcctccttac
    16741 gcaggtagat ttctccgcct ctggcgcgga agttctccga catatcagcc attcccactt
    16801 caatggtttg cgcggcggcg taatcgcgca cttcctggct gattttcatc gagcagaatt
    16861 tcggcccaca catggagcaa aaatgggcga ctttgccgga ttcttgcggc agggtttcat
    16921 cgtgataagc gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa
    16981 attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgccctttcg
    17041 ccaggtcagc ggcgtgggcg gcaatcttat aggtgataag cccctgctta acatcttctt
    17101 tattaggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc
    17161 agccaatcat cgccgcacca atccccgacg tgaagtggtc ataacccggc gcgatatcgg
    17221 tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca
    17281 tattgcggcg gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc acctgtacgt
    17341 catattccca ggcgattttg gtcagttcac ccagcgtatg cagctcggca aattgcgctt
    17401 catcgttggc gtcctgaata gaaccagggc gcagaccgtc tcccagcgac agcgaaacgt
    17461 cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat
    17521 gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc
    17581 gtttcgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca
    17641 caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg
    17701 cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccgggc
    17761 tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca
    17821 ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag
    17881 aggtgaccgc cgagttgccg atattggcgt taacttttac caggaaattg cgaccaataa
    17941 tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcgcgt ccggcagcaa
    18001 cttcatcacg gacaaattcc gcagtgatat tttccggcag acgtgcgcca aagctcattc
    18061 ccggatgctg gtggcgtaaa acttcgctac gtatgtgctc gcggcccata ttctcgcgga
    18121 tggcgatgaa ttccatttcc ggtgtgatga tgccctggcg ggcgtagtgc agttgggtga
    18181 cgcggcgtcc tgctttggcg cgttttggtg ttagtacgcc gctaaaacgc agctcgtcga
    18241 ggccatcatc tgccagccgc gctttagtgt aatcggaact gcgcacggta agttcttcgg
    18301 tatcgccgcg cgcatcgatc cacggctggc gtagttttgc cagtccttgc tgcacgttaa
    18361 tggcgatctg tggatcgcca tacggaccgg aggtgtcgta gaccggaatc gcttcgtttt
    18421 cttcgtactg cggttgttct ttgctgccgc caattagcgt cgggctaagc tggatctcac
    18481 gcatcggcac gcgcacgccg gggtgtgtgc cagtgatgta aatgcgtttt gagttgggaa
    18541 aggcggtgcc ttccagggtg tcgataaaat gttgggcctg ggcgcgttgt tcgcggcggg
    18601 tcagttttgt tacagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac
    18661 ggatgaagca agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc
    18721 ttcgcaggta ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta
    18781 aggcactccg acaagaaatt agccccacaa acggggcgtt gaatgtaaat tacgcgttaa
    18841 cagcgcagaa ctcaagcagg atgtttgacg cgggcggcgt caagtaccag cagaatcaac
    18901 ttatcttcca gcacaaagcg tgcttccagc gcctcgccga tgtcagataa aacctgttga
    18961 aactcaaggt aattatcatg atcgatggcg gtttccaggc tggaatcgta gtaatccata
    19021 atctgttggg tattggcttc gagttgcggc caaatcttcg cggcgcgcgc gagttgcccg
    19081 ttgccttcca gcttatgaag aatgcgctca taaatactga aatgtccggc agacaagtaa
    19141 tcgaccaggc tctgacaaaa atcatcaagg gctttttcgt ttagcctcat gtacgattct
    19201 ttgccaggct taatgccaac cagattgtag taagccacga gcagatgctt acgtacatgt
    19261 agccagcgat caaccagttt gttacttcct ctgacgcgtt ccgtcaggtt atcgagctgg
    19321 ttaagcatga ttgactccgc aagtttgtat tcaaaaactg ctcagtgaga aatgtaaaaa
    19381 ccatgttaaa catgccagtg atgcaaaggt agtgcaagag ctatggatcg tataattgaa
    19441 aaattagatc acggctggtg ggtcgtcagc catgaacaaa aattatggtt gccgaaggga
    19501 gaattgccat atggcgaagc ggcaaatttc gatcttgtgg gtcagcgcgc actacaaatc
    19561 ggcgaatggc aaggggaacc tgtttggtta gtacaacagc agcggcgtca cgatatgggg
    19621 tcggtacgtc aggtcattga tctcgatgta gggctgtttc aactggccgg acgtggcgtg
    19681 caactggcgg agttttaccg atcgcataaa tactgtggtt actgcgggca tgaaatgtat
    19741 ccaagcaaaa ccgaatgggc gatgctgtgc agccattgcc gtgagcgtta ctacccgcaa
    19801 atcgccccct gcattattgt tgccatccgt cgcgatgatt cgatcctcct cgcccagcat
    19861 acccgccatc gtaacggtgt ccatacagta cttgccggat tcgtcgaagt gggtgaaacc
    19921 ctcgagcagg cagtcgcgcg ggaagtgatg gaagagagcg gaattaaagt taaaaacttg
    19981 cgttacgtga cctcccaacc gtggccgttt cctcagtcgt taatgaccgc gtttatggcg
    20041 gaatatgaca gcggcgacat cgttatcgac ccgaaagaat tgctcgaggc gaactggtat
    20101 cgctatgacg atttgccgtt actcccgccg cccggcaccg tagcgcgccg tctgatagaa
    20161 gatacggtgg cgatgtgtcg ggcagagtat gagtgatgat acactgaccg cctgacgcac
    20221 taaggaacag ccaaaatgac cgaacttaaa aacgatcgtt atctgcgggc gctgctgcgc
    20281 cagcccgttg atgtcactcc agtatggatg atgcgccagg cgggtcgcta tctaccggaa
    20341 tataaagcca cgcgcgccca ggcgggcgat tttatgtcgc tgtgcaaaaa tgccgagctg
    20401 gcgtgcgaag tgactttgca accgctgcgt cgctatccgc tggatgcggc gatcctcttt
    20461 tccgatatcc tcaccgtgcc agacgcgatg ggtttagggc tctattttga agccggagaa
    20521 ggtccgcgtt ttacctcacc agtcacctgc aaggccgacg tcgataaact gccaattccg
    20581 gacccggaag atgagctggg ttacgtgatg aacgcggtgc gtaccattcg tcgcgaactg
    20641 aaaggcgaag tgccgctgat tggtttttcc ggcagcccgt ggacgctggc gacctatatg
    20701 gtggaaggcg gcagcagcaa agcgttcacc gtgatcaaaa aaatgatgta tgccgatcca
    20761 caggcgctgc acgcgctact cgataaactg gcgaaaagcg tcactttgta tctgaatgcg
    20821 cagattaaag ccggtgctca ggctgtgatg attttcgaca cctggggcgg cgtgcttacc
    20881 gggcgcgatt atcaacagtt ctcgctctat tacatgcata aaattgttga tggtttactg
    20941 cgtgaaaacg acggtcgccg cgtaccggtc acgctgttta ccaaaggcgg cggacagtgg
    21001 ctggaagcca tggcagaaac cggttgcgat gcgctgggcc tcgactggac aacggacatc
    21061 gccgatgcac gccgccgcgt gggcaataaa gtcgcgttgc agggcaatat ggatccgtcg
    21121 atgctgtacg cgccgcctgc ccgcattgaa gaagaagtag cgactatact tgcaggtttc
    21181 ggtcacggtg aaggtcatgt ctttaacctt ggtcacggca ttcatcagga tgtgccgcca
    21241 gaacatgctg gcgtgtttgt ggaggcagtg catcgcctgt ctgcgcctta tcatctgtaa
    21301 ggagtggtta tggatctcgc gtcattacgc gctcaacaaa tcgaactggc ttcttctgtg
    21361 atccgcgagg atcgactcga taaagatcca ccggatctga tcgccggagc cgatgtcgga
    21421 tttgagcagg gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata cccctcgctt
    21481 gagctggtgg agtataaagt ggcccgcatc gccaccacca tgccttacat tcctggtttt
    21541 ctctcctttc gtgaatatcc tgcgctgctg gcagcgtggg agatgctgtc acaaaagccg
    21601 gatttagtgt ttgtcgatgg tcatgggatc tcgcatcctc gccgtcttgg cgtcgccagc
    21661 cattttggtt tgctggtgga tgtgccgacc attggcgtag cgaaaaaacg gctctgcggt
    21721 aaattcgaac cgctctccag cgaaccgggc gcgctggccc cgctgatgga taaaggcgag
    21781 cagctggcct gggtctggcg cagcaaagcg cgctgtaacc cattgtttat cgctaccggc
    21841 catcgggtca gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa aggctatcgt
    21901 ctgccggagc caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc gttcgtgcgc
    21961 tatacagcaa atcagcccta attcaggtaa actgcggata atttccgtat ttgagaactc
    22021 aacatgttac aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg gcagcacgtc
    22081 acttttatgg cctgcttatg cgaacgtatg taccccaatt acgccgtatt ctgtcagcaa
    22141 accggcttcg gcgatgggca gatttaccgc cgcattctcg atctcatttg ggaaacgctg
    22201 accgtcaaag acgcaaaagt aaatttcgac agccaactgg agaaatttga agaagcgatt
    22261 ccttcagccg acgatttcga tctgtacggc gtttatccgg caatcgatgc ttgcgtggcg
    22321 ttaagtgaac tggtccattc gcgtttaagt ggcgaaacgc tcgaacacgc ggtggaagtg
    22381 agtaagactt ccatcacgac cgtggcgatg ctggaaatga ctcaggctgg tcgcgaaatg
    22441 agcgatgaag agctcaaaga aaacccagct gtagagcaag aatgggacat tcagtgggaa
    22501 atattccgac ttttagctga gtgcgaagaa cgcgatatcg agctgataaa aggccttagg
    22561 gcagacctgc gtgaggcggg tgagagcaat attggtataa tttttcagca ataagaccag
    22621 aaaacgtgat ttaacgcctg atttgtcgta cctggagtct tccctttcgc cccccgtctg
    22681 gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg agagtgctat
    22741 tctggcattt ccgtcgcact cgatgcttag caagcgataa acacattgta aggataactt
    22801 atgaacaaga ctcaactgat tgatgtaatt gcagagaaag cagaactgtc caaaacccag
    22861 gctaaagctg ctctggagtc cactctggct gcaattactg agtctctgaa agaaggcgat
    22921 gctgtacaac tggttggttt cggtaccttc aaagtgaacc accgcgctga gcgtactggc
    22981 cgcaacccgc agaccggtaa agaaatcaaa attgccgcag ctaacgtacc ggcatttgtt
    23041 tctggcaagg cactgaaaga cgcagttaag taagattgcg tggcagtgaa cagttttaac
    23101 gaaggggtgg tttcacccct tttgtctttc tggcgtcgat cattgatgct ggctggcgct
    23161 ctgtttctca ctgcttgtag tcacaactct tcacttcctc cctttaccgc cagtggattt
    23221 gctgaagacc agggcgcggt acgcatctgg cgaaaagaca gcggcgataa tgtgcatctt
    23281 cttgccgtgt ttagcccgtg gcgcagtggc gatacgacga cgcgagagta tcgctggcag
    23341 ggcgataacc tcacgctcat caatatcaat gtttacagca aacccccggt gaatattcgc
    23401 gcgcgttttg acgatcgcgg cgatctgagc tttatgcaac gcgaatccga tggggaaaag
    23461 cagcagcttt ctaacgacca aatcgattta taccgttatc gtgctgatca gatccgccag
    23521 attagcgatg ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg gcatgcgatg
    23581 gagcagactg tgaccacctg cgaagggcaa accattaaac ctgatttaga ttcgcaggcg
    23641 atagcgcata tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt ggcatggctg
    23701 gaagcgcccg aaggttcgca attactgttg gtggcaaact ctgatttctg tcgctggcaa
    23761 cccaacgaga aaacgttctg atttaccagt ggtccatacc catatgaccg ccaccaccgc
    23821 agccgccgta acccattccg gtaccgcgcg gaatacccgc ttcagccatc gcaatatctc
    23881 gtttcacccg taactcatct aacgactgac gcaaattctc catctctttg gcgaccgcgt
    23941 taattttgct gctatccggt gggttcgcgg ctaacagggc attgtattca taacgctttg
    24001 ttaccagttg ctgttgcagt gcgctgcttt gagcgtaaaa gtcattatgg attttctgcc
    24061 acgctgtctg ttgttcgctg gtcaaaggcg cggcattttg ctgccacata ccgtgtccgc
    24121 cgtgagcaaa tgccgatgtc gatcccatcg ccattgctga aagcgccatc attaccaggg
    24181 caattttcgt gttccgtttc atggttaatc ctccagtggt tgtcttactt cgggtattgc
    24241 atcttccgtg ccaacgatga aacgctgata tgacgggtaa tctggcatga taaacgagta
    24301 aaaatgactc gcctgctgcg ggtagcgagt catttttact cattgaaact taagcctttg
    24361 tgttacagcg cagggtaagc gctgataaaa gatggcatga tttctgctgt cagaaaggga
    24421 tgagcaggca aagaagaaga tgcgttttat gcaacgttct aaagactcct tagctaaatg
    24481 gttaagcgcg atcctccccg tggtcattgt tgggctggtg gggctgtttg cggtgacagt
    24541 gattcgcgat tacgggcgcg agactgcagc cgccagacaa acgctgctgg aaaaaggcag
    24601 tgtacttatc cgtgctctcg aatccggctc gcgcgtcggc atggggatgc gcatgcatca
    24661 tgcgcagcag caggcattac tggaagaaat ggccgggcag cctggtgtac gttggtttgc
    24721 ggtcacggat gaacaaggaa caatcgtgat gcatagcaac tccggcatgg tgggaaaaca
    24781 gctttattcc ccgcaggaaa tgcagcagtt acatccggga gatgaagaag cgtggcggcg
    24841 gatcgatagc gcagacggcg agcctgttct ggaaatttat cgccagtttc aaccgatgtt
    24901 tgctgctgga atgcaccgga tgcgccatat gcagcagtat gccgcgacac cacaagcaat
    24961 ttttatcgct ttcgacgcca gtaacattgt gagtgccgaa gatcgtgagc agagaaacac
    25021 cctgattatc ctcttcgccc tggcgacggt cttgctggca agcgtgttgt cattcttctg
    25081 gtatcgccga tatctgcgct cgggccagct gttgcaggat gaaatgaagc gcaaagagaa
    25141 gctggtggca ctggggcatc ttgcggcagg cgttgcccac gaaatccgta acccactttc
    25201 ctcgattaaa gggctggcga aatactttgc cgaacgcgcg ccagcagggg gagaagcgca
    25261 tcaactggcg caggtgatgg cgaaagaagc cgaccgttta aaccgcgtgg taagcgagtt
    25321 gctggaactg gttaagccaa cgcatctggc tttgcaggcg gtggatctca acacgctgat
    25381 taaccactca ttacagctgg taagccagga tgcaaacagc cgggagatcc agttacgctt
    25441 taccgccaac gacacattac cggaaattca ggccgatccg gacaggctga ctcaggtcct
    25501 gttgaatctc tatctcaatg ctattcaggc gattggtcag catggcgtga ttagcgtgac
    25561 ggccagcgaa agcggcacgg gtgtgaaaat cagcgttacc gacagcggta agggaattgc
    25621 ggcggatcag cttgaagcca tcttcacccc gtatttcacc acgaaggccg aaggcaccgg
    25681 actggggctg gcggtcgtgc ataatattgt tgaacaacac ggtggtacaa ttcaggtcgc
    25741 aagccaggtg ggaaaaggcg caacgttcac cctctggctt ccggtcacta ttacgcgtaa
    25801 ggacccacaa ggatgacgca cgataatatc gatattctgg tggtggatga tgacattagc
    25861 cactgcacta ttttgcaggc tttgctgcgc ggctggggct ataacgtcgc gctggcgaac
    25921 agcgggcgac aggcgctgga gcaggtgcgg gaacaggttt ttgatcttgt gctttgcgat
    25981 gtgcgaatgg cggagatgga cggcattgcc acgctaaaag agatcaaagc gctaaatccg
    26041 gcgattccgg tactgattat gaccgcatac tccagcgtcg agacggcggt agaggcactg
    26101 aaaaccgggg cgctggatta tctcatcaag ccgctggatt tcgataacct gcaggcgacg
    26161 ctggaaaagg cgctcgcaca tacgcacagt gttgacgctg aaacgcctgc ggtgtctgcc
    26221 agccagttcg gtatggtagg taaaagcccg gcgatgcaac acctgctcag tgaaatcgcc
    26281 ctcgtcgcgc catcggaagc cacagtgctg atccacggcg attccggcac cggtaaagag
    26341 ctggtcgcaa gggcgattca cgccagtagc gcacggagtg aaaaaccact ggtaacgctc
    26401 aactgtgcgg cactcaacga atccttgctg gaatctgaat tgttcggtca cgaaaaaggg
    26461 gcgtttaccg gagccgataa acgccgggaa gggcgctttg ttgaggcgga cggcggcacg
    26521 ctgtttctcg atgaaattgg cgatatctcg ccgatgatgc aggttcgtct gctgcgtgcg
    26581 attcaggagc gcgaagttca gcgtgtcggc agcaaccaga ccatctcggt tgatgtccgg
    26641 ctgattgcgg cgactcatcg cgatcttgcc gcagaggtga atgccgggcg ttttcgccag
    26701 gatctctact atcgcctgaa cgtggtggcg attgaagttc cgtcgctgcg tcagcggcgg
    26761 gaagatattc ctctgctggc aaaccatttt cttcagcgct ttgccgagcg taatcgtaag
    26821 gcggtaaaag gttttacgcc ccaggcgatg gatctgctga ttcactacga ctggccggga
    26881 aatattcgtg aactggaaaa cacggtggaa cgggcggtgg tgctgctgac cggggaatat
    26941 atttccgaac gcgagctgcc gctggcgatt gcgagtacgc cgatcccgct ggtacaaagt
    27001 caggatattc agccgctggt ggaagtcgaa aaagaggtga ttctggcggc gctggagaaa
    27061 acgggcggca acaaaaccga agccgcccgc cagttaggga tcacgcgcaa aacgctattg
    27121 gcaaaactgt cgcgttagtt ctgctcgcgt tcgatagcgc gccagccgat atctttccgg
    27181 cagaagcagt cgtcccaatg gatatcggtc attaaggcat aagcgcgttt ctgcgcttct
    27241 gccacggtat gacccagcgc ggtaacgcac agtacgcgcc cgccgctggt cactacctgc
    27301 tcgtcatccg ctagtttcgt gcccgcgtgg aacactttgc cgtctgccac ttcttccagc
    27361 ggcaggccgt ggatcacatc gccggtgcgg taatcgcccg gatatccacc cgcagccatc
    27421 accacgccga gagaagcgcg ttcatcccac tcggacgttt tctcgtccag cttgccttca
    27481 caggccgcca ggcagagctc aaccagatcg gacttcatgc gcagcataat cggctgggtt
    27541 tctggatcgc caaagcggca gttaaattca ataaccttcg gattgccctg tttgtcgatc
    27601 atcaggcccg cgtagagaaa accagtgtag gtgttgcctt ctgccgccat gcctttcacg
    27661 gttggccaga tgattcgttc catcgtgcgc tgatgaactt catcggttac taccggcgcg
    27721 ggggagtaag cgcccatccc gcccgtgttt ggtccggtat ctttatcgcc tacgcgtttg
    27781 tgatcctggc tggtagccat cggcagcaca tgctcgccgt ccaccatcac gataaagctc
    27841 gcttcttcgc catcgaggaa ctcttcgata acgatgcgat gacccgcgtc gccaaacgcg
    27901 ttgcccgcca gcatatcgtg aacagctgct tcagcttctt ccagcgtcat cgccacgata
    27961 acgcctttcc cggctgccag accgtcagct ttaatgacga ttggcgcgcc tttctcacgc
    28021 agatacgcca gcgcaggttc tacctcggtg aagttctggt attccgctgt gggaatgtta
    28081 tggcgggcca gaaagtcttt ggtgaatgct ttagaacctt ccagttgtgc ggcacctgcg
    28141 gttgggccga agattttcag cccggcggcg cggaaggtat cgactacgcc tttcaccagc
    28201 ggcgcttccg ggccgacgat ggttagatcg atcttttcgt tttgcgcgaa atccagcagc
    28261 gccgggatat cggttacgcc gatagcgacg ttttgcagtg tgggttccag cgcagtgcct
    28321 gcattacccg gagcaacaaa aacagtttca accagcggcg actgggccgc tttccaggcc
    28381 agcgcgtgct cgcgcccgcc gttaccaatc actaatactt tcatctgtcg ctccattaat
    28441 ggcggaagtg gcgcatgtcg gtgaagagca tcgcaatacc gtgctcgtcg gcggcggcaa
    28501 tcacttcgtc atcacggata gaaccgccag gctggattac gcaggtcaca cccgcagcgg
    28561 cggcggcatc aataccgtcg cggaacggga agaacgcgtc agaagccatc gaggaacctt
    28621 tcacttccag gccttcatcg gccgctttaa taccggcgat ttttgcggag tacacgcggc
    28681 tcatctggcc cgcgccaatg ccgatggtca tattgttttt ggcatagaca atggcgttgg
    28741 atttcacgaa cttcgccact ttccagcaga acagcgcatc acgcagttcc tgttcggtcg
    28801 gctgacgttt ggtgacgacg cgcagttctt ctgcaccgac catgcccagg tcgcgatcct
    28861 gaaccagcag accgccgtta acacgtttga aatcaagacc cggaacacgc tcgccccact
    28921 gaccgcaggt caggacgcgt acgttctgtt tggcggcggt gattttcagg gcttcttcgc
    28981 tggcggacgg cgcaataatc acttcaacaa actgacgaga aatgatggcc tgcgcggttt
    29041 ccgcatccag ctcgcggtta aaggcgataa tgccgccgaa tgcggaggtc gggtcggttt
    29101 tgtacgcgcg atcgtaagca tcaagaatgg aattgctgat agccacgccg cacgggttgg
    29161 cgtgcttcac aatcacacat gctggctcgg cgaactcttt cacgcactcc agcgccgcat
    29221 cggtatcggc gatgttgtta taagagaggg ccttaccctg aacctgggtt gcggtggcaa
    29281 cagaggcttc tttcacgttc tcttctatat agaaggcagc ctgctggtgg ctgttctcgc
    29341 cgtaacgcat atcctgcttc ttaatgaagt tcaggttcag cgtgcgtggg aagcgaccgg
    29401 cggcttcttt gctttcaccg tggtaagccg gaaccatgct gccgaagtag ttggcaatca
    29461 tgctgtcgta ggcggcggta tgttcaaagg ctttgatagc gagatcgaaa cgggttgcaa
    29521 gcagaagcga tccttcgttg gcatccatct ctttaatgat ggcgtcgtag tcgctgctct
    29581 tcaccacgat ggcgacatct ttatggttct tggcggcaga gcgcaccatc gtcgggccgc
    29641 cgatatcgat gttctcaacc gcatcttcca gcgagcaacc ttcacgggca acggtctggg
    29701 cgaacggata caggttaaca accaccatat cgataggctg gatctgatgt tcttccataa
    29761 tggcatcgtc ctggccgcga cggcccagaa tgccaccatg tactttcgga tgcagggtct
    29821 tcacgcgtcc atccatcatc tccgggaaac cggtgtaatc ggaaacttcg gttaccggca
    29881 gacctttttc tgctaacaga cgggctgtgc cccctgtaga cagcagctcc acaccgcgtg
    29941 cggaaagtgc ctgggcgaat tcgacgatac cggctttgtc agaaacactg agcagagcgc
    30001 ggcggactgg acgacgttgt tgcatggtaa atcccctgga tttgactatt acagagagcg
    30061 ttagctgaat ttttcgcgaa aaactcagct aacgccccta acggggcatc cttatttttc
    30121 gcccgcattg taacgaaaac gtttgcgcaa cgctcgcgaa tttttctctt tcaatggtga
    30181 tcacaatttt gacagtggct accgtgggaa aaatacagaa attacattga tgattgtgga
    30241 taactctgtg cgtaaaaagg tataaagcgg ggttttgctg gggaatgcag cagtcagtca
    30301 tttttatgca atttttctat tgcgacctgc ggagaactcc ctataatgcg cctccatcga
    30361 cacggcggat gtgaatcact tcacacaaac agccggttcg gttgaagaga aaaatcctga
    30421 aattcagggt tgactctgaa agaggaaagc gtaatatacg ccacctcgcg acagtgagct
    30481 gaaagccgcg tcgca
//
LOCUS       DAZGHF010000049        30417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGHF010000049 DAZGHF010000000
VERSION     DAZGHF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.049"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            158..1954
                     /locus_tag="ACHK3A_002317"
     CDS             158..1954
                     /locus_tag="ACHK3A_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFO8740383.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1972..2640
                     /locus_tag="ACHK3A_002318"
     CDS             1972..2640
                     /locus_tag="ACHK3A_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFO8740384.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSLA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2655..3326
                     /gene="dsbI"
                     /locus_tag="ACHK3A_002319"
     CDS             2655..3326
                     /gene="dsbI"
                     /locus_tag="ACHK3A_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFO8740385.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIVRG"
     gene            complement(3372..4160)
                     /gene="ygiD"
                     /locus_tag="ACHK3A_002320"
     CDS             complement(3372..4160)
                     /gene="ygiD"
                     /locus_tag="ACHK3A_002320"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO8740386.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4303..5076
                     /gene="zupT"
                     /locus_tag="ACHK3A_002321"
     CDS             4303..5076
                     /gene="zupT"
                     /locus_tag="ACHK3A_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO8740387.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(5134..5304)
                     /gene="yqiD"
                     /locus_tag="ACHK3A_002322"
     CDS             complement(5134..5304)
                     /gene="yqiD"
                     /locus_tag="ACHK3A_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO8740388.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(5566..6219)
                     /gene="ribB"
                     /locus_tag="ACHK3A_002323"
     CDS             complement(5566..6219)
                     /gene="ribB"
                     /locus_tag="ACHK3A_002323"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO8740389.1"
                     /translation="MNQTLLSSFGTPFERVENALVALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(6323..6471)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6593..6892
                     /gene="ubiK"
                     /locus_tag="ACHK3A_002324"
     CDS             6593..6892
                     /gene="ubiK"
                     /locus_tag="ACHK3A_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO8740390.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(6927..7127)
                     /gene="glgS"
                     /locus_tag="ACHK3A_002325"
     CDS             complement(6927..7127)
                     /gene="glgS"
                     /locus_tag="ACHK3A_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO8740391.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            7396..8025
                     /gene="yqiJ"
                     /locus_tag="ACHK3A_002326"
     CDS             7396..8025
                     /gene="yqiJ"
                     /locus_tag="ACHK3A_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO8740392.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCLMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            8052..9713
                     /gene="yqiK"
                     /locus_tag="ACHK3A_002327"
     CDS             8052..9713
                     /gene="yqiK"
                     /locus_tag="ACHK3A_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO8740393.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            9953..10012
                     /locus_tag="ACHK3A_002328"
     CDS             9953..10012
                     /locus_tag="ACHK3A_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8740394.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(10132..11565)
                     /gene="hldE"
                     /locus_tag="ACHK3A_002329"
     CDS             complement(10132..11565)
                     /gene="hldE"
                     /locus_tag="ACHK3A_002329"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO8740395.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNQ
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(11613..14453)
                     /gene="glnE"
                     /locus_tag="ACHK3A_002330"
     CDS             complement(11613..14453)
                     /gene="glnE"
                     /locus_tag="ACHK3A_002330"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO8740396.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFLQD
                     SIIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPFDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(14476..15777)
                     /gene="ygiF"
                     /locus_tag="ACHK3A_002331"
     CDS             complement(14476..15777)
                     /gene="ygiF"
                     /locus_tag="ACHK3A_002331"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO8740397.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            16019..16639
                     /gene="ygiM"
                     /locus_tag="ACHK3A_002332"
     CDS             16019..16639
                     /gene="ygiM"
                     /locus_tag="ACHK3A_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO8740398.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            16703..17941
                     /gene="cca"
                     /locus_tag="ACHK3A_002333"
     CDS             16703..17941
                     /gene="cca"
                     /locus_tag="ACHK3A_002333"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO8740399.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVAGWKEQRCPKPE"
     gene            complement(18129..18950)
                     /gene="bacA"
                     /locus_tag="ACHK3A_002334"
     CDS             complement(18129..18950)
                     /gene="bacA"
                     /locus_tag="ACHK3A_002334"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO8740400.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(19041..19409)
                     /gene="folB"
                     /locus_tag="ACHK3A_002335"
     CDS             complement(19041..19409)
                     /gene="folB"
                     /locus_tag="ACHK3A_002335"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO8740401.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            19514..20131
                     /gene="plsY"
                     /locus_tag="ACHK3A_002336"
     CDS             19514..20131
                     /gene="plsY"
                     /locus_tag="ACHK3A_002336"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO8740402.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(20144..21076)
                     /gene="ttdR"
                     /locus_tag="ACHK3A_002337"
                     /gene_synonym="dan"
     CDS             complement(20144..21076)
                     /gene="ttdR"
                     /locus_tag="ACHK3A_002337"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO8740403.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            21283..22194
                     /gene="ttdA"
                     /locus_tag="ACHK3A_002338"
     CDS             21283..22194
                     /gene="ttdA"
                     /locus_tag="ACHK3A_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO8740404.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            22191..22796
                     /gene="ttdB"
                     /locus_tag="ACHK3A_002339"
     CDS             22191..22796
                     /gene="ttdB"
                     /locus_tag="ACHK3A_002339"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO8740405.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            22845..24308
                     /gene="ttdT"
                     /locus_tag="ACHK3A_002340"
     CDS             22845..24308
                     /gene="ttdT"
                     /locus_tag="ACHK3A_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO8740406.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(24351..25364)
                     /gene="tsaD"
                     /locus_tag="ACHK3A_002341"
     CDS             complement(24351..25364)
                     /gene="tsaD"
                     /locus_tag="ACHK3A_002341"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO8740407.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            25602..25817
                     /gene="rpsU"
                     /locus_tag="ACHK3A_002342"
     CDS             25602..25817
                     /gene="rpsU"
                     /locus_tag="ACHK3A_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO8740408.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            25928..27673
                     /gene="dnaG"
                     /locus_tag="ACHK3A_002343"
     CDS             25928..27673
                     /gene="dnaG"
                     /locus_tag="ACHK3A_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO8740409.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKQAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDNQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            27868..29709
                     /gene="rpoD"
                     /locus_tag="ACHK3A_002344"
     CDS             27868..29709
                     /gene="rpoD"
                     /locus_tag="ACHK3A_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO8740410.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            29922..>30417
                     /locus_tag="ACHK3A_002345"
     CDS             29922..>30417
                     /locus_tag="ACHK3A_002345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740411.1"
                     /translation="MFFLCVGKNKGWRKVQKASDYGVIILTEEQFINFIDTGEIPLEQ
                     DCVVENDISKSLEKDVNTYKSMIDEFSLSIRTVREFRRSNALIAVFVDGYAAGWRFAV
                     KECYKDSLDIKLTPVKTEFFNYEIWTQGDSFQFNRGDVFYSDRLAYDSYCDFLKLDDA
                     VVLQV"
BASE COUNT         7570 a         8043 c         7586 g         7218 t
ORIGIN      
        1 tgtttcaccg aataacaaca tatttaaaag aaacacaaaa gcattctcac tctttattga
       61 attaatattt atattcacgt aacgaattta ttagcaccct ctcaacagtc gatattcatc
      121 attattatat atttgcgtta tataaggaga tatatccatg tttgataaat atagaaaaac
      181 actcgtagcc ggaactgtgg cgataaccct gggtttgtca gcatcggggg tgatggctgc
      241 gggttttaaa ccagcgccgc ctgccgggca actgggtgcg gtcattgtcg atccctacgg
      301 caatgcacca ctgaccgctt tggttgactt agatagccat gttatttctg acgtcaaagt
      361 taccgtccat gggaagggcg aaaaaggtgt agaaatcagc tatcccgtgg gtcaggaatc
      421 actaaaaact tacgatggtg taccgatttt tggtctttat cagaaatttg ctaacaaagt
      481 gaccgttgag tggaaagaaa acggcaaggt catgaaagat gattatgtgg tgcacacttc
      541 ggccatcgtc aataattaca tggataaccg ctctatatcc gatttacaac agaccaaagt
      601 tattaaagtc gcaccgggtt ttgaagatcg cctctatctg gttaataccc acacctttac
      661 cgcccaaggt tccgatctcc actggcatgg tgagaaagat aaaaatgccg gtatccttga
      721 tgcgggtccg gcaaccggcg cactaccttt tgatatcgcg ccctttactt tcatcgtcga
      781 taccgaaggt gaataccgct ggtggctgga tcaagacact ttctacgatg gtcgtgaccg
      841 cgatatcaac aagcgtggtt atctgatggg tatccgcgaa acgccacgcg gcacctttac
      901 cgctgtacaa ggtcagcact ggtacgagtt cgacatgatg gggcaggtgc tcgaagatca
      961 caaactaccg cgcggatttg ctgacgccac tcatgagtcc attgagacgc caaatggcac
     1021 ggtactgttg cgcgtaggta agagtaacta tcgtcgcgat gacggcgtac acgtcaccac
     1081 cattcgtgac catatcctcg aagttgataa atctggtcgc gttgtcgatg tatgggatct
     1141 gaccaagatc ctcgatccga aacgcgatgc actgctcggc gcgctggatg caggtgcagt
     1201 ttgcgttaac gttgaccttg cccatgcagg acaacaggca aaactggaac cagatacacc
     1261 gttcggcgac gcgctgggtg tagggccagg ccgcaactgg gcgcacgtga actctatcgc
     1321 ctatgacgca aaagatgact ctatcatcct ctcctctcgc caccagggtg ttgtgaaaat
     1381 tggtcgtgat aagcaagtga aatggatcct tgcgccctct aaaggttggg aaaaaccgct
     1441 ggccagcaag ctgctgaaac cggttgatgc taacggtaag ccaattacct gtaacgaaaa
     1501 tggcctgtgc gaaaactcag acttcgactt tacctacacc cagcataccg cctggatttc
     1561 cagcaaagga acgctcacca tttttgataa tggcgatggg cgtcatctgg aacaacctgc
     1621 cttaccaacc atgaaatact cccgctttgt ggaatataag attgatgaga agaaaggcac
     1681 cgttcagcaa gtgtgggaat acggtaaaga acgtggctac gatttctata gcccgattac
     1741 ctccatcatt gaatatcaag ccgaccgtaa caccatgttt ggcttcggtg gttctattca
     1801 tttgttcgat gtcgggcagc caaccgtcgg taagttgaac gaaatcgatt acaaaaccaa
     1861 agaagtgaaa gtggaaatcg acgtgctgtc agataaaccc aatcagactc actatcgtgc
     1921 actgttagtc cgtccacaac agatgttcaa ataattaaga agggaataaa aatgtcgaaa
     1981 ttagggatct catcactatt taaaaccata ctgcttaccg cagctttagc tgtttcattt
     2041 accgcttctg catttaccga aggcacggat tacatggttc tggaaaaacc tattccgaat
     2101 gccgacaaaa cgctgattaa ggtattcagc tacgcctgcc cgttctgtta caagtacgac
     2161 aaagcggtta ccggtccggt atcggaaaaa gtgaaagata tcgttgcttt cacgccgttc
     2221 catctggaaa ccaaaggtga atacggcaaa caagcaagcg aagtctttgc ggtcttgatc
     2281 aataaagata aagcagcagg catttcctta tttgatgcta actctcagtt taagaaagcc
     2341 aagtttgcct actacgcggc ttatcacgac aaaaaagaac gctggtctga cggtaaagat
     2401 ccggctgctt ttatcaaaac cggtctggat gccgcaggta tgagcttggc ggattttgaa
     2461 gctgcactga aagaaccagc ggttcaagaa acactggaaa aatggaaagc ctcttatgat
     2521 gtcgccaaaa tccagggggt tccagcctat gtcgttaatg gtaagtactt gatctacacc
     2581 aagagcatca aatccatcga cgcgatggca gaccttatcc gtgagttagc aagcaaataa
     2641 cagggaaaca ggctatgggt attaagggaa tgtggaaaga ccttcgcaca tcaccagttg
     2701 acacactggt cagatggcag gagcaacgct tgttgtggtt gttaatggcc gttgcgatgg
     2761 gcgcgttaat tatcctggcg cactctttct tccagatcta tctctacatg gcaccctgtg
     2821 agcaatgtgt atacattcgc tacgccatgt ttgtgatggt tattggagga ttggttgcag
     2881 cgatcaaccc aaagaacatc atcctaaagc taattggctg cgtgatggca ttttacggca
     2941 gcattttggg actgaagttt tcgctcaaac taaacgatat ccaccatgca gtacataatc
     3001 cggatcccga ttcactgttt ggcgtacagg gttgctctac cgatccaact tttcccttta
     3061 acctgccgct ggcacagtgg gcaccaaatt ggttcaagcc tacaggggat tgtggctatg
     3121 acgcaccaat cgttcctgat ggcgtcacac tgagcagcac gcaacagtgg tttgtggaaa
     3181 tgtatcagca atctgaaggc tggtatctgc taccaccatg gcattttatg aatatggctc
     3241 aggcttgtat gctggctttt ggtatgtgtc tggtgctgct ggtcattatg agtggcgcat
     3301 gggcgcttaa aatcgtcaga ggctaactca gcaacacccg aagccagtcg ttctggcttc
     3361 gggttctttt attagcctat ctgcaccgac aacatactta aactgcccat ttctatacca
     3421 tcgaccggaa tggtaattgg ctcctgccca tcccacgcac ctaagacata caacaacggc
     3481 agatagtgct ctggcgttgg gttcgataac gcgccacctt catggtcgag ataattcacc
     3541 agaggatgct gctctaccgg cccttgccac gtcagattcg ctttcacata ctcattaaac
     3601 gacgtcgccc acggatacgg tgaactatca ccgtgccact tcactgtgcg caggttatgc
     3661 accacgttac cgctggcgac caacattatt ccttcatctc gcagcgccgc gagtttgcgc
     3721 cccatttcga aatgccaggc ggcaggtttg ctactgtcga tactcaactg caccatcggg
     3781 atatccgcgt caggatacat cttaatcagc acgccccagg aaccgtggtc aaagccccag
     3841 gcttctttat ccagcgtcac cgggatcggc gctaacagct caaccagacg ctgtgccagc
     3901 gcaggcgaac ccggagcagg ataatgcgta tcgtacagcg cctgcgggaa gccaccaaag
     3961 tcatgaatcg tgggcggcgt ctccatcgcg gtcactcctg ttccacgggt aaaccagtga
     4021 gccgaaacca ccacaatcgc ttgcgggcgt ggcaatgtca tccccaacgt ctgccagctg
     4081 cgggtataca aattatcttc cagcacgttc atcggactac cgtgacctaa aaacaatgct
     4141 ggcatacgtg ttgaagacat gatgatatcc ttaactaaag gtgtcatttt gatatcctca
     4201 caatacgctt gttcggcgga gtaagaaccc ggataacaat gatgatgatc atcagttatt
     4261 ttgacgatct gcctgaaggt gaagatttat aaggagttgt cgatgtcagt acctctcatt
     4321 ctgaccatac tggcgggggc agccacgttt attggcgcgt ttctcggcgt tctcgggcaa
     4381 aaaccctcga accgcttact ggcgttttcg ctaggatttg cggcggggat catgttgctc
     4441 atctcattaa tggaaatgct tcctgccgca ctagccgccg aaggaatgtc gcctgtgttg
     4501 ggttatggaa tgtttatctt cggtctgctt ggctattttg gcctggaccg catgttgcca
     4561 catgcgcatc cgcaggattt aatgcaaaaa tcggtgcagc cgttgccaaa atcgatcaag
     4621 cgcacagcca ttctgctcac tctcggcatc agtctgcata acttcccgga agggattgcc
     4681 acctttgtca cggcgagcag caacctggag ctgggctttg gcatcgcact ggccgttgcg
     4741 ttacacaata ttcctgaagg tctggcggtt gcaggcccgg tttacgcggc aacggggtct
     4801 aaacgcaccg caattctttg ggcggggatt tctggactgg cagaaattct tggtggtgtg
     4861 ctggcgtggt taatcctcgg tagcatgatt tccccggtgg tcatggcggc aatcatggcg
     4921 gcggttgcag gaattatggt ggcgctctcg gttgatgaat taatgccgct cgccaaagag
     4981 attgacccta ataataaccc cagctatggc gtactatgtg gaatgtcagt gatgggattc
     5041 agtttagtgc tgctacaaac ggcgggaatt ggttaaaagc aacatcagaa gttgtccagc
     5101 gcatggacaa cttctgccag cttcactaaa gccttattct accttttcgg ctgtctcttc
     5161 tctcgtactg tttaagtatt tgttccgcgc ttcaagcagt gcgtctctgt cctggcgaaa
     5221 cgcccgacaa taacgtttca aatgcaggaa ataacccacc acaacaagag caatcaatac
     5281 aatccagtac caggcgataa acatcctttt acccttaata tttaacaata ctaaacattt
     5341 agcgcataaa tttcacatat cctttttcgg atatattcat atggtcgcat aataacggaa
     5401 caattatatg attttgatca taatgaaaaa gaatgcctga tatcaatgaa attttctcat
     5461 agaaagagaa gcggaattaa catcttgcat agcagggcaa aataaaaacc gaagccagct
     5521 acaacttcgg tttcttgatt aagacagtaa attaagcagc gattttcagc tggctttgcg
     5581 ttcatgcgcc tgacgataag ccaccaggtc ttcaatagtc acgagcgcca tattgtgttt
     5641 attggcaaac tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat tagtcagctc
     5701 acacagcaca ccagccggtt taaagccagc cagcgtcatc agatcaatag tcgcttcagt
     5761 atggccgcca cgcgtcagta caccaccagc ctgagcgcga agtgggaaaa cgtggcccgg
     5821 gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg tcgtaatacg
     5881 gtcagcggca gaaacaccgg tagtcacacc ttcagctgct tcaatggtca cggtaaaacc
     5941 ggtgccatag gcgctggtgt tattttctac catcattggc agatcgagtt gtttacggcg
     6001 atcttcagtg atgcacaggc aaacaatacc gctaccgtgg cgaatggtca gcgccatctg
     6061 ctcaacggtc atggtttctg ccgggaagat catatcacct tcgttttcac ggtcttcatc
     6121 atcaagcacc attacaccgc gtccttcacg cagcgcaacc agtgcatttt caacacgttc
     6181 gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaac ctcactaaaa
     6241 ttatggttac cagaatcagg gcagtcttag gagtggcggc atatagccaa aataacgtga
     6301 gcgggtccat gcccgacaga atcgttactc tctcccatcc ggactctaac cgtcggcccc
     6361 ggaattacac cggatctgct gtcctttgag ttcgcaccca aagcgctcgc gggctttcaa
     6421 ctgagttgat ttaccgccgg tggggaattt cgccccgccc tgagaataag cggattcact
     6481 ataacgctaa tgattagcgg cagcaacgca tagcttcaca taattctggt ttatgactta
     6541 cccttatcgc actacaatgg cactcaacac ctatcagtac agggaaacta caatgattga
     6601 cccgaaaaaa attgagcaaa tcgctcgcca ggttcacgaa tcaatgccta aaggaatcag
     6661 ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagcgc agctgacgcg
     6721 tctcgatctg gtaagccgcg aagaattcga cgttcagacg caagtcctgt tacggactcg
     6781 tgaaaaactg gcgttactgg agcagcgcat cagtgaactg gaagcgcgca ataattcggt
     6841 agcagatctt caatctcctc cggctatccc gcctattgat aaagcagaat aaagcatcac
     6901 aaaaacgggc cagacggccc gtaatatcag tgttctaatt caagttctct ggtttgcatc
     6961 atctgttggc aataccaggc ataacgtgcg caaaaccagg ttctatgttc ttcatccatg
     7021 ttaccagtaa cggccagaat atcgaccggg cgaccttctg cgtgcattct ggcaaaacta
     7081 cgcgccagga aatcgaaatt atttaaagaa ttaagactat gatccataag cacatcctcc
     7141 ggttatcgca taccttttaa ggccatatgc tttttctgat ttcagtattc cccaatcagt
     7201 gtagtgcctt taaacataac gtgcgtaaat atattgtccc cgatcacact ttttagtgaa
     7261 aaataaaaaa ccatattaaa agactttctc ttccagaact tctctaacat gtgttattat
     7321 taacaaagat aaatgtaagc aaggaaacta tcctaaagta aatattgcgt cactaaatgg
     7381 acatcggagt gacatatgat tttattcgcc gactataaca ccccttatct tttcgctatt
     7441 tcctttgtac tgttgatcgg ccttttagaa atttttgcgc ttatctgcgg tcacatgctc
     7501 tccggtgctc ttgatgcaca tcttgaccat tatgattcca taaccacagg tcatattagc
     7561 caggcacttc attatctcaa tatcggaaga ttgccagctc tcgtcgttct ctgcttactg
     7621 gctggttttt ttggtcttat tggcatcctg ttgcagcata cctgcctcat ggtctggcaa
     7681 tcaccactct cgaacctgtt tgtggttcct gtcagtttac tgtttacgat tattgcggtg
     7741 cattataccg gtaaaatagt tgcgccctgg attccacgcg atcacagctc agctattaca
     7801 gaagaagaat atattggtag catggcgtta attaccggtc atcaagccac ttcaggtaat
     7861 ccctgcgaag gaaaactcac cgatcaattt ggccaaatac attatttact actggagcct
     7921 gaagaaggaa aaattttcac taaaggagac aaagtgttaa ttatttgccg actttctgca
     7981 actcgatatt tggcggaaaa caatccctgg ccccaaatat tgtaatttat taatcaaaag
     8041 gaaatttaat aatggatgat attgttaatt ctgtgccctc ctggatgttt accgcgatta
     8101 ttgccgtatg cattctgttt attattggaa ttattttcgc caggctctat cgtcgcgctt
     8161 cggcagagca agcttttgtt cgtactggtt taggtgggca aaaagtggta atgagcggtg
     8221 gcgcaatcgt gatgccgatc tttcatgaaa taatccccat caatatgaat actctgaagc
     8281 tggaagtcag ccgctcaacc attgatagcc tgattacgaa agatcgtatg cgcgtcgatg
     8341 tcgttgtcgc tttctttgtg cgggtaaaac cgtcagtgga aggtattgcc accgccgccc
     8401 agacgcttgg gcaacgcacc ctgtcgcctg aagacttacg tatgttggtt gaagataaat
     8461 ttgtcgatgc cctccgtgca acagctgcac aaatgaccat gcatgagtta caggataccc
     8521 gcgagaactt cgtgcagggc gtacaaaata ccgtagctga agatctgtcg aaaaacggcc
     8581 tggaactgga aagcgtttca cttaccaact ttaaccagac gtcgaaagaa catttcaatc
     8641 ctaacaacgc ctttgacgcc gaaggtttaa ctaagctgac gcaggagacg gagcgccgtc
     8701 gtcgcgaacg taacgaagtt gaacaggatg tagaagttgc ggtgcgtgag aaaaaccgtg
     8761 atgcgctttc gcggaagctg gagattgaac agcaagaagc gtttatgacg cttgagcagg
     8821 agcagcaggt taaaacccgt accgctgagc agaatgcgaa aattgcggct tttgaggccg
     8881 agcgtcgtcg ggaagcagag cagacgcgaa ttctggctga acgacagatt caggaaacgg
     8941 aaatcgaccg cgaacaggcc gtccgttcca gaaaagttga agctgaacgt gaagttcgca
     9001 ttaaagagat cgaacagcag caggtcaccg aaatcgccaa ccagacgaaa tcgatcgcga
     9061 ttgccgccaa atcggaacag cagtcacaag cggaagcgcg tgccaacctt gcacttgcag
     9121 aagcggtaag cgcccagcaa aacgtagaaa ccactcgcca gactgccgaa gccgatcgtg
     9181 ctaaacaagt ggcgttgatc gccgcagcgc aggatgcaga aaccaaagcg gttgaactga
     9241 ccgtgcgggc gaaagcagaa aaagaagccg cagaaatgca ggcggcggct atcgttgagt
     9301 tagccgaagc tacacgtaaa aagggtctgg cggaagcaga agcacaacgt gcgctgaacg
     9361 atgctatcaa cgtactttct gatgaacaaa ccagccttaa attcaaactg gcattattgc
     9421 aggcgctgcc tgcggtaatt gagaaatccg tggaaccaat gaagtctatc gacggcatca
     9481 aaattattca ggtcgatggg ctgaatcgtg gtggcgctac gggtgatgca aacacgggta
     9541 atgtgggggg cggaaacctg gcagaacaag cgttatcagc cgcactctct taccgtacac
     9601 aggcaccgct gattgactcc ttgctcaatg aaattggcgt ttcaggcggc tcactggcgg
     9661 cattgacttc atccttaagc tcaacaactc ccgtcgaaga aaaagcagag taaaccctct
     9721 tctatgaata atccctggcc caaaaagccg gggatttttt atatctgcgt tccgctaaaa
     9781 ggtgcaaatg atcaagctgt tgcagcattt tgcgtgactg ctcggggaag gcaaaactgc
     9841 ctctgggaaa gcgttgcgcg gggtccggcg ctcatcaaca atcggggagt cagcaagggg
     9901 ctgaaacggg aaagcccctc ccgaggaagg ggccttgtat aaggaaaggg ttatgatgaa
     9961 gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt aacagccaat
    10021 cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga aaaaaaatca
    10081 gcgtcaatag gcccgcctgc tacaaagcga gatctgtgaa ccgctttccg gttagccttt
    10141 tttatcctgt tggatcttct tgatgatgtt ggtcgtcgag caaccgtctt caaagttgag
    10201 caccagcact tcaccaccgt tggcccagac ttctttactc ccggcaatct cttctggttt
    10261 atagtcgccg cctttcacca gtagatctgg caagataccg gcgatcaagc gctgcggcgt
    10321 gtcctcttca aacgacacca cccagtcgac cgcttccagt gcgcccagca caatcatacg
    10381 ctgttcgagc tggttaacgg ggcgggaatc ccctttcagc cgtttggtgg aggcatcgct
    10441 gttcacagcg acaatcaaac ggtcacccag tttacgggca tttgccagat aagagacgtg
    10501 cccagcgtgc aggatgtcaa agacaccgtt ggtcatcacc actttttcac cgcgtttacg
    10561 cgctgccgct acagcaagct tcagttcctc ttcggtcatc acaccaaagc cggtatctgc
    10621 acgtccacgt acagcgtttt ccagctcgat cggcgaaacc gtggatgtcc ccagtttgcc
    10681 gaccaccaca ccagccgccg cattggcaaa gaagcaggct tcttccagtg aattacccgc
    10741 tgccagcgtt gccgccagga cgccaatcac cgtgtcgccc gcaccggtaa cgtcatacac
    10801 ttcctgcgcc tgggttggca tatgcagtgg cgctttaccc ggttgcagca gcgacatacc
    10861 ctgttcggaa cgggtcacta acagagccga gagttcgtaa tcggcaatca gtttcatgcc
    10921 gcgctcaaca atctcttctt cggtcttaca tttaccgaca acagcttcaa attccgagag
    10981 attcggcgtt aacagcgtag cgccgcggta gcgctcaaaa tcggtacctt ttggatcaat
    11041 cagcaccgga acacccgctt tacgcgccag ttggatcatc tgctgtacgc ttgccagcgc
    11101 acctttggcg taatcagaaa gcaccagcgc gccaatcgaa ctcagtgcct gattaatccg
    11161 ctcgtgcagc ggctgcggat caaccccttc gaaaccttct tcgaaatcca gacggatcag
    11221 ttgttggttg cgggaaagta cccgtaattt ggtgatggtc ggatgcgtcg gtacagaaac
    11281 aaagtcgcat ttgacgttga cgtcggccag agatttactg agcgcgcgcg ccgcatcgtc
    11341 aatgcccgtc aacccgacca ggcgtgcatt agcaccgaga gaagcgatat tcatcgccac
    11401 gttagccgcg ccgcccggac gttcttcgat ggtattcact ttaaccacgg gcaccggcgc
    11461 ttccggcgag atacggctgg tgggaccgta ccagtaacga tccagcatca catcaccaac
    11521 caccatcact cctgcacgtt caaactctgg cagcgttact ttcattcctg tctcctgaga
    11581 gattcaaaat ttgcgcgcga taataccata cttcattctt ccaccagcca cttctgccag
    11641 cttgcccgca ccagttcacg ctctgcggtg aagcaatcct ccgacacatg acccggcaat
    11701 tcctgtaatg ccagatgatg aagttcatcg cgcagcgtcg tgtaagcacg ggtcagcgcc
    11761 atcgcttcct gctcttccat aatgtcgttt tgcgccagta gttccagaat gcgcacgttg
    11821 tctgaccagc gcgttaactt cggtttttcg tgagcgtagc gcaacaccag atattgagta
    11881 ataaattcga tatcggtaat tccgccttca tcggctttga tatcaaagcg atcgcgatgt
    11941 ttattgccga gatgggcacg cattttctcg cgcatttccc gcacttctgt ttgcagggtt
    12001 ttgccttcgc gcggcagcgt cataatctcc cggcgtactg cgtcaaagtg cgcggtgagc
    12061 tgcggatcgc cgtacactac acgcgcacgc accagcgcct gatgctccca cgtccaggcc
    12121 tcgtttttct gataatcagc aaatgcttct gcggatgtca ccagcattcc cgccgccccg
    12181 gacggacgca gtcgtgcatc cacttcatac aaaatgccgg aagaggttcg cgtactgaac
    12241 agatgcataa tgcgttgcgc cagacgcaga taaaactgtc gcccgtcgat ttcccgctcg
    12301 ccgtcagtca tcgcatccat tgggcaatca tggaggaaga tcaggtcaag atcggaactg
    12361 tagcctaact cccagccacc cagcttgccg tagccgacca ccgcaaaacc acgcccttcg
    12421 cgttcgttca ggtgatttgg cttaccgtag cgggcaacca tttgaaccca cgcctgctga
    12481 acgacggcat caatcatggc ttctgccagc caggttaagt gatcgctcac tttcattacc
    12541 ggtagcgtac cggcgatatc cgctgccgca atgcgtaaca gctgcgcctg tttgaactga
    12601 cgcagcgcct caagctgttg ttcttcgtca tcttccggca cgcgcagcaa atactggcgc
    12661 agttcatcac ggtaggcatc cgtcgccgtt ggttggtaga gggtatttgg atcgagcaat
    12721 tcatccagca ataatggata acgcgctagc tggctggcaa tcatcggcga tgcagcacac
    12781 agagaaatca aatgtttaag cgccgcaggg aattcactaa gcagctcaag ataagtggtg
    12841 cgagtaacaa tccccaccag caaggcggta atgcgcgaca gcgtgacagc agcgtcttca
    12901 cgcgcacaga catcacttag cagatgtggc atcagatggt cgagcacctg acgtcctcgc
    12961 ggcccgatgg tgcgcttatc cagctctttg cggaaatcag caatcagcat tagcacctgt
    13021 ttgcgatcat cctcgctaag atgcgccagc actggcgtgg tgtcatcttc ctgcaacgca
    13081 tcctgccaca gctcacgcca ctgttccgac agcgactctt cctgagtttc actttcgtcg
    13141 tcgccaatca attcattaaa cacccggcgc acattggcca tatgtgcggt cagcaccccg
    13201 gtcaattgcg gccagtcagc aaagtccatc gcccacgcca gccgcgcacg gttaagctca
    13261 tcaaaaggaa gcgtctgggt ttgttcgtcg ttaatgcttt gcagcaggtt ttccagacgc
    13321 cgcaggaaca gatacgccac tcgcaattgt tcagcatcgt tttcagaaag cagatgcagc
    13381 gcggcaatgg cgctgagcgt tggcagtaaa gagcgcgatt gcagcgacgg ttcgcgcccg
    13441 ccgcgaatga gctggaacac ctggacgata aattcaattt cgcgaatgcc gcctgcgcca
    13501 agtttgatgt tgtcggtcaa accgcgtcga cgcacttcac gggcaatcat tcctttcatg
    13561 ttgcgcagcg actgaatcac gctgaaatcg atgtaacgac ggaaaacaaa cggacgcagc
    13621 atcgcacgca actcgttagc atagacgccg tcgctatccc ccataatccg cgccttgacc
    13681 atcgcgtaac gctcccagtc gcgcccctgc tcctggtaat aatcttccag cgccgcaaaa
    13741 ctcagcacca gcgggccact ttcaccaaac ggacgcagcc gcatatccac gcgatagacg
    13801 aaaccatcct gcgttggttg atccagcact ttaatcagcc gctgccccat gcgggtaaaa
    13861 aactgcgcgt tatccagttc ccggcgtccg ccctgggtac aaccatgttc cggccaggca
    13921 aaaatcagat cgatatctga ggagaaattc agttccccac cgcccagctt acccatgcct
    13981 aaaatcagca gcggttgcgc ttcgccctgc gcattgcacg gcgttcccca ttcgcggcag
    14041 caggcgtcat acagccagtc acgcgccgca acaatcagcg tctccgccag atggctgagc
    14101 tgctgcaata tgctctcttc agtaaccagt gccagcgttt gcgcccaggc gatgcgcacc
    14161 ataatgcgcc gccggaatag ccgcagctcg cgcattaacc cggcttcgtc actcacatta
    14221 ctgagcgcct cctgcaacca tgctgcgtaa tgctgccatt cgtcggcctg cggcggttgg
    14281 ctttccagtt ccgtcagcca ctctggatgc gcaattatgc tgtcctgcag aaaatcactg
    14341 aaagtaagta ctgacttcgc ctgtgcgcta agtgattcct cggctaaagg ctctggcagc
    14401 cgctcaacaa cggtctgcca gtactgctgt aacggtgaag agagcggctt cataagcgat
    14461 tttatccttg cctgattaac gttttccgct gtgcaaccag aacggttcct gattgtttgc
    14521 ctcattacgg aaatgttcaa tttcaatgcg ttgcccggtc gcaatagcgt gacgcagccc
    14581 ctgccagttc tccagccagg cttgcgcgac gacaggatcg tagtaacccg ccagcaacag
    14641 gattgagtca atatcacgcg tcaggcgtgg caactggtca cggtagcgat cgcccaacgg
    14701 ctggcagaaa acgcttttca gttcagcggc atggcgggaa agatggatat cggcaaagcg
    14761 tttgaaggaa tcgctcattt tactctgcgc tttggcatct aaaaacggct gccaggcttt
    14821 gcttaccagc cattcggtca atgccagctt cgccattgcc gtttcggtag agtagaccgc
    14881 cgtcacggca gaaaccgctg aagcgatggt cgcttcgcat tgagtcaata aatcacgtaa
    14941 gtgagtgctc gctttacgcg gcacaatgcc accgaacagc atcagcgtat gacggaccag
    15001 gccaatggct gccagcacct gctctttcgc cgcatcattg ccgcgtaccc atagttcttc
    15061 atgatactgc cattgcgcta acgccagctc gagcgccgct tccagcccct gttccacatc
    15121 agcttttgcc gcaacatgta aaatggtggt cggtttgatt tcacgcgccg gattgccctg
    15181 cgccagatga tagccacgcg ccgctttgct cagactgccc tggcgtaatc cggtttgcga
    15241 taccagttgg ttcgccagtt tcagcaccgc gcgcgtgtcg ccgctaagca gttccagttc
    15301 cagctcgcag ataggttcag caaattcacc cgctttcact tccccctggt cgagggcgat
    15361 ttcaatttgg ctaccatcaa cctctaccag ccacttttcg cgataaaaat cggtgctaaa
    15421 cagcggctgc acgcgggaag cgagatcggt aggcaattcg ccgttcggcc agacttccgt
    15481 cggtaactgc gccaggtcga gcgtcggttc gctcaacgcc acgttatatt ccgggcgctg
    15541 gtgtaagccg cctgtcactc ttcctgcaac tttcatggtc atctcatagc gaccgttttc
    15601 gccacgaata cgcaagccca tgtcgtgccc acgcagccag ttatccggcg tttcgtagta
    15661 aatattcagc aactgcacgg ggtcatggtg ctcgccgccc agcgtattga gatggtcacg
    15721 caacgcctca acggcactgt gattaacaat aaactttaat tcgatttcct gagccatagc
    15781 ctttacttat cggttatgtc acataagtga cgatgaacgg cgaacttaat gcgatctttt
    15841 ttgtcagtag atagtatttt gcgccaaatt gccatgcaac gggcaatttg acgggcgtaa
    15901 aagtttgaag cagtggcaag acctgacaca gaggattccg attgatgtcg aatgctttgc
    15961 gtagcgacac tgatgccact actatcgttc cactttccat gacaataacg acagcctgat
    16021 gccaaaatta cgcctgatcg gattaacttt actcgcactt agcgccactg ccgtctcaca
    16081 cgctgaagaa acgcgctatg tttctgatga actgaatacc tgggtccgta gcggtccggg
    16141 agatcattat cgcctcgtgg gcacggttaa cgccggcgag gaagtgacct tattacaaac
    16201 tgacgccaac accaattatg cccaggtgaa agacagctct ggccgtaccg cctggatccc
    16261 gttgaaacaa cttagcacag agccaagcct gcgctcccgt gtgccagatc tggaaaatca
    16321 ggtcaaaacc ctgaccgata aactcaccaa tatcgataac acctggaacc aacgcaccgc
    16381 agaaatgcag caaaaagtgg cgcagagcga cagcgtgatc aacgggttaa aagaagaaaa
    16441 tcagaaactg aaaaacgagc tgattgtcgc gcagaaaaag gtcgatgccg ccagcgtaca
    16501 gctggatgac aaacagcgca ccatcatcat gcagtggttt atgtatggcg gtggcgtgct
    16561 ggggcttggc ttgctgcttg gtctggtact gccgcacctg atcccaagcc gcaaacgcaa
    16621 agatcgctgg atgaactaaa tcgccttctt cgttgcactg tcttattatt aggaattgtg
    16681 tatttgacga agggatgatg gcgtgaagat ttatctggtc ggtggtgctg ttcgggatgc
    16741 attgttaggg ctaccggtca aagacagaga ttgggtggtg gtcggcagta cgccacagga
    16801 gatgctcgac gcgggctacc agcaggtagg ccgcgatttt cctgtgtttc tgcatccgca
    16861 aacgcatgaa gagtatgcac tggcacgtac cgaacggaaa tctggttccg gttacaccgg
    16921 ttttacctgc tatgccgcac cggatgtcac gctggaagat gatcttaagc gtcgcgatct
    16981 gaccattaat gcgctggccc aggacgataa cggcgagatt atcgacccgt acaacggtct
    17041 gggcgatctg caaaatcgtc tgttgcgcca tgtttccccc gcttttggcg aagatccgtt
    17101 acgcgtgttg cgcgtagcgc gttttgctgc gcgttatgcc cacctcagtt tccgtattgc
    17161 cgatgaaact ctggcgctga tgcgcgagat gacccatgcg ggtgaactgg aacacctgac
    17221 gcctgaacgg gtatggaaag agacggaaaa cgccctcacc acccgcaatc cccaggtgtt
    17281 cttccaggta ctgcgcgatt gcggcgcact gcgcgtttta ttcccggaaa ttgacgccct
    17341 gtttggcgtt ccggcccctg ccaggtggca tccggaaatc gacacgggta ttcatacctt
    17401 aatgacgctc tcaatggcgg cgatgctgag tccgcaggtc gatgtccgtt tcgcaacgtt
    17461 atgccacgat ctcggtaaag gactgacgcc accagaactt tggccgcgtc atcatggtca
    17521 tggcccggcg ggtgtgaagt tagtggaaca actatgccag cgtctgcgcg tgccaaatga
    17581 aattcgcgat ttagccagac tggtggctga gtttcacgat ctcatccaca ccttcccaat
    17641 gctgaaccca aaaaccatcg tcaaactatt tgattccatc gacgcctggc gtaaaccgca
    17701 gcgtgtcgag caactggcgc tgaccagcga ggctgatgtg cgcggcagaa ccggttttga
    17761 atcagccgac tacccgcaag gccgctggtt gcgcgaagcc tgggaagtgg cacagtcagt
    17821 gccgacaaaa gccgtcgttg aagcgggatt taaaggtgtg gagattcgcg aggagctgac
    17881 ccgacggcgg attgcggcgg tggctggctg gaaggaacag cgttgcccaa aacctgagtg
    17941 agtgttggat attttgccgg atgcgacgta agcgtcttat ccggctacat gtcaacgaca
    18001 gttgtaggcc tgataagacg cgcgagcgtc gcatcaggcg ttgattgccg gatgcggcgt
    18061 gaacgcctta tccggcctac gcaatctgat aagacgcgtc agcatcatat ccgacactgt
    18121 tcttcacatc aaaagaacac aacgtacacc gcagccgcca caataaagcg ataaatggcg
    18181 aacgggataa acgaaatgcg cttaatcaat tgcaggaagg ttttaatcgc tatcagcgcc
    18241 accacaaaag cggtgataaa cccaacggca aacatcggga tatcgccggt agtcaggaag
    18301 ccccagcttt tgtagagatc gagcgccgtt gcgcccatca tcatcggcac cgccagcagg
    18361 aacgagaact cggaagcagc gtaacggctc acccccatca gcatcccacc tgaaatggtc
    18421 gccccggaac gggagaaacc cggccacagc gccagacact ggaaacagcc aatcataaac
    18481 gcctgacgat aggtcatatc atcaagaccc ggcgcacgcg gctctttcgg cttcaggcat
    18541 tcggcggcaa tcagcaacag accgccaacg accagtgcat acatcacatt tatcgggtta
    18601 aacaatgatt taatcgtgtc gtggaacaac agccccagta ccaccgccgg aatcatcccc
    18661 agcaaaatgt ggatcagcgt taaacggcct ttgctttctc cttcgtgctg caacgggcga
    18721 ccaaagtgaa taccaataag gccaaacaga cgtcgccaga acatgacaac cactgccaga
    18781 attgatccta actggatcac gacttcaaag gttttcgccg tgtcgccctc aaaccccaac
    18841 aagtgaccga caataatcat atggcccgtg ctggataccg gcagaaattc tgtcaatcct
    18901 tcgaccacac ccaatattgc cgctatcagc agcgagtgca tatcgctcat caataaaccc
    18961 ctaaattata aaaatgtaac gcttgtccga actactgcag tatgaccagg ttataaccgt
    19021 ttggtttaac agctgtaaaa ttaattattt tctttcagat tattgccacg ctcaatgatt
    19081 acgccaacat tcgccgcccg cgccactgcg cctggcttgc tgagtttgat acgcacccac
    19141 ggcgagttga agcgtgctaa cagcaactcc gccacctctt cagccacacg ttccaccagc
    19201 gcaaaacgcg ccccctcgac gtggctgact accgtttctg caatgtcagc gtaactgagg
    19261 caatccgcca catcatcact tttcgccgct ttacggttat cccacgccat ttcgatatcg
    19321 aacactaact tctgttcgat ggtctgttcc cagtcgtaaa caccaatagt ggtgattacc
    19381 gaaagttgct ctataaatac aatatccatc acgtcctgcc tgctttttgg ctaaccggat
    19441 accacttccg gcgaaatgtg cgtattatcc acagattcat cgttgaacac gaattttcaa
    19501 aacggaacag cttatgagtg caatcgcgcc tggaatgatc ctcatcgcgt acctctgcgg
    19561 ctccatttcc agtgccattc tggtttgccg cttgtgtggg ctgcccgatc cgcgaaccag
    19621 cggctccggc aatccaggcg caaccaatgt gttacgtatc ggtggcaagg gagcagccgt
    19681 agcagtactg attttcgacg ttctgaaagg aatgttgccc gtctggggcg cgtatgaatt
    19741 aggtgtcagc cccttctggc taggcttaat tgccatcgcc gcctgtcttg gacacatctg
    19801 gcccgttttc ttcggattta aaggaggaaa aggcgttgct accgcttttg gtgccatcgc
    19861 acccattggc tgggatctca ccggagtaat ggcgggaacc tggttactga ccgtgctatt
    19921 gagcggatat tcgtcgctgg gggcgattgt cagtgcactg attgctccgt tttatgtctg
    19981 gtggtttaag ccacaattca ccttcccggt ttcgatgctc tcttgcctga tcctgctgcg
    20041 ccatcatgac aacatccagc gtctgtggcg tcgtcaggag acaaaaatct ggacgaaatt
    20101 caaaagaaag cgcgaaaagg atcccgagtg atttctggtg gagctacata acctgatagc
    20161 cttcgtcggg cttgcccagc cgttgctggc accatgccgc cagaaattcc acgcagacac
    20221 gtaatttcat gctgcgatag agcggctccc ggtaaacagc ccagatattg gcgctttgtg
    20281 catactccgg caatactcgc accagtttgc cactctccag aaacggcaac acatcccact
    20341 cggaacgcaa cataatccct ttgccctcca gcgcccattg caacactatc tcgccgctat
    20401 tggaggaaag atgcccgctt accttcaccg attttttctc ctgaccgttc cccaactccc
    20461 atattccgtg ggtcatatcg cgctctttgg tcaccaggca gtcatgacga cttaattcct
    20521 gtaaggattg tggctgcgga tatttttgca gatactcagg tgctgcgcat aatattcttt
    20581 tattttttgt caaaagatgc gcaatataat aatcaggaat tgcgtcatta atacgaatat
    20641 ccagatcaat attatcctgc actaaatcaa tttgccgatc gaacaattca aaatgcacct
    20701 gtaactcagg ataattgcgc atcagttcgg taatggctgg agcaatatgg ctgcgcccaa
    20761 aaccaaaact gcaaccaatg cggatcatcc cctccgggcg cgttttgatt tgcgtgacgt
    20821 catcgaccag tcgctgatat tgggtgagga tttccagcgc atgttcgtaa caacgttggc
    20881 cactttcagt cagcgccacg ccgcgagcgg agcggttcag cagagttgtt gccagcgtat
    20941 tttcaaggat ttggatccgc ttggtgacaa atgcaggcgt ttgcccaagc gtcgccgctg
    21001 cagcactaaa actcccgcta tggacgattt ccaccagtac ctgtaagtct ttggctaaag
    21061 gccagctatt tagcattgtt aaatatccgc agttttgata aacgcagtgt atttcgcaaa
    21121 acattaccat tccctgctct ggcgcaatat tcgttcttat tttcgcgttg attcacgaac
    21181 tgttaattac atattcacgg tggcaaaaaa tataaaacca catttttagt ggtagtttgt
    21241 ggcggtgaat ttttccagac aaatacataa actggagttg ccatgatgag cgaaagtaat
    21301 aagcaacagg cagtgaataa gttgacggag attgtcgcta actttaccgc catgatttct
    21361 acccgaatgc ctgatgacgt ggtggataaa ctaaaacagc taaaggatgc cgaaacatcg
    21421 tcgatgggaa aaatcatcta ccacacgatg ttcgataaca tgcaaaaagc gatcgacctg
    21481 aatcgtcctg cctgtcagga caccggcgaa atcatgtttt ttgttaaggt cggttcccgt
    21541 ttcccactgc ttggcgagct gcaaagcatc ctcaaacaag ccgtggaaga ggcaaccgtc
    21601 aaagcgccac tgcgtcacaa tgcggtagaa atttttgacg aagtgaacac cggcaaaaat
    21661 accggcagcg gcgtaccgtg ggtcacctgg gatatcgtcc ccgacggtga cgatgcggaa
    21721 atcgaagttt acatggcagg cggcggctgc acactacctg gtcgttcgaa agtgttaatg
    21781 ccgtcggaag gatacgaagg cgtggtgaaa ttcgtcttcg aaaatatctc caccctcgcc
    21841 gtaaacgcct gtccaccggt actggtaggc gtgggcatcg ccacctcggt ggaaaccgcc
    21901 gctgtgctct cacgtaaagc cattttacgc ccaattggca gccgccaccc caatccgaaa
    21961 gcggcagagc tggagctacg cctggaagaa ggactcaacc gtctggggat tggtccacaa
    22021 gggctgactg gcaacagttc agtgatgggc gtacatatcg aatctgccgc ccgccatccg
    22081 tcaaccatcg gcgttgctgt ttctacaggc tgctgggcgc atcgtcgcgg cacactgctg
    22141 gttcatgccg atctcacctt tgaaaatctg tctcacaccc ggagcgcgtt atgaaaaaga
    22201 tcctgacaac cccgatcaaa gctgaagatc tgcaagatat tcgcgtcggc gatgtgatct
    22261 acctgaccgg tacgctggtg acctgccgtg acgtttgtca ccgtcgcttg atcgaactca
    22321 aacgtccgat cccttacgat ctcaacggca aagcgatttt ccacgctggc cccatcgtgc
    22381 gcaaaaacgg cgacaaatgg gagatggtat ccgtcggccc gacaaccagt atgcgtatgg
    22441 aaagttttga acgtgaattt atcgaacaga ccggcgtgaa actggtggtc ggcaaaggcg
    22501 gaatggggcc gctgaccgaa gaaggctgcc agaaattcaa ggcgctgcat gtgattttcc
    22561 cggcaggctg cgcagtactg gcggcaaccc aggtggaaga gattgaagaa gtgcactgga
    22621 cagaactcgg gatgccggag tcgctgtggg tctgccgggt caaagagttc ggtccgctga
    22681 ttgtttctat tgatacccac ggcaacaacc tgatagccga aaacaaaaag ctgttcgccg
    22741 aacgccgcga tcccatcgtg gaagagattt gcgagcacgt ccactacatc aaataaccct
    22801 cccggagagg ctcaccccct ctcctttttc gcaggcataa cacgatgaaa ccttccactg
    22861 aatggtggcg ataccttgcg ccgctggcgg tcatcgccat tattgctcta attccggttc
    22921 ccgcagggct ggagagtcat acctggctct actttgccgt ttttactggc gtgatcgttg
    22981 gactgatcct cgaacccgtg ccgggtgccg tggtggcgat ggtgggtatc tccattatcg
    23041 ccattctctc gccgtggctg ctgtttagcc cggagcagct cgcccagcca ggttttaagt
    23101 tcaccgccaa atccctctcc tgggcggttt ctggtttttc taattcggtt atctggctga
    23161 ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac cgggcttgga cgccgcattg
    23221 cgctgattct ggtgaaaaag atgggacatc gcacgctgtt tctcggctat gcggtgatgt
    23281 tctccgagct gatcctggca cctgtaacac cgtccaactc ggcgcgcggt gcggggatta
    23341 tctatcccat catccgtaac ctgccaccgc tctatcaatc acaaccaaac gacagcagtt
    23401 cgcgcagcat tggctcgtac atcatgtgga tggggattgt tgccgactgc gtgaccagcg
    23461 ccattttcct gacggcgatg gcacctaact tgctgttaat tggcctgatg aaaagcgcat
    23521 ctcacgccac actgagttgg ggcgactggt tcctcgggat gttgccgctc agtattttac
    23581 tggttctgct ggttccctgg ctggcttacg tgctgtaccc gccggtactg aagtctggtg
    23641 atcaggtgcc gcgctgggca gagacggaac tgcaggcaat gggcccgctc tgttcgcgtg
    23701 aaaaacggat gctggggctg atggtaggcg cgctggtgct gtggattttc ggcggtgatt
    23761 atatcgatgc cgcgatggtc ggttacagcg tggtggcgct gatgctgctt ctgcgcatta
    23821 tcagttggga cgacattgtc agtaataaag ccgcgtggaa cgttttcttc tggctggcct
    23881 cgcttatcac cctcgctacc ggactcaaca acaccggttt tattagctgg tttggcaaac
    23941 tgttagcagg cagcttaagc ggttattcgc caacgatagt gatggtggcg ttgattgtgg
    24001 tgttttatct actgcgctac tttttcgcca gcgccacggc gtatacctcc gctctcgcgc
    24061 cgatgatgat tgccgccgcg ctggcgatgc cggaaatccc gctgccggtg ttctgcctga
    24121 tggttggcgc ggcaattggt ctggggagca ttcttacgcc atacgccacc ggacccagtc
    24181 cgatttacta cggtagtggt tatctgccaa cggtggatta ctggcgactg ggggcaattt
    24241 ttgggctgat attcctcgtg ttgctggtga ttaccggctt actgtggatg cccgtggtgt
    24301 tgctttaagt gaggacggtg ccggaagtga ctctccggca cctaagagtt ttacgcggcc
    24361 ggtaactctg ccagcggcca gcgcggacgc acgctaacgc caagatccgc cgtcgcgcct
    24421 gctttaaacc gcaccattcc ggcataggcg atcatcgcgc cgttatcagt acaaaattcc
    24481 ggacgcgcgt agaacacttc gccacggcgt tttttcatca tttccgccag cttcgcccgt
    24541 aacgtgcggt tagcactcac gccacctgcc attaccagtc gcttaaagcc cgtctgatcc
    24601 agcgctcgct tacacttaat catcaacgta tcgaccaccg catcttcaaa ggcgcgggcg
    24661 atatcagcac gcgtctggtc gtcggtgccg ttatcacgaa tggtatttgc cgcgaaggtt
    24721 ttcagaccag aaaagctgaa atccagccct ggacggtcgg tcatcgggcg cgggaagaca
    24781 aagcgcccgg ctgtaccctg agccgccatt ttcgacagta atggcccgcc aggataatcc
    24841 agccccagca gcttcgcggt tttatcaaac gcttcaccag ccgcatcatc gatagactcg
    24901 ccgagcagct cgtactgacc aatgccagtc acgctgatta actgcgtatg accgccggaa
    24961 accagcagcg ccacaaacgg aaattccggt gggttatctt ccagcatcgg cgctaacaga
    25021 tgcccttcca tatggtgtac agggatcgcc ggaacgttcc aggcaaacgc cagagaacgc
    25081 cccacggtcg cgccaaccag cagtgcgccg actaatccag ggcctgcggt ataggccaca
    25141 gcatcaatgt cttttgccgt taaaccagac tcctttagcg ccgcctggat caacggtacg
    25201 gttttacgca catgatcgcg ggaggccagc tcaggcacta cgccgccgta gtcagcgtgc
    25261 aatttcacct gactatacaa ttggttggct aacaaacctt tttcatcgtc gtaaatggcg
    25321 atgccggttt catcgcagga agtttcaata cccagtacac gcatgacttg ttttacctcg
    25381 ctttattacc gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt tcgcccctgg
    25441 agaaagcctc gtgtatactc ctcaccctta taaaagtccc tttcaaaaaa ggccgcggtg
    25501 ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt
    25561 gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac
    25621 gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag
    25681 gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc
    25741 gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc
    25801 gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg
    25861 taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc
    25921 ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga
    25981 catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc
    26041 gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt
    26101 ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga
    26161 caagcttgag ttcgtcgaaa cggtcgaaga gctggcggca atgcacaatc ttgaagtgcc
    26221 atttgaagca ggtagcggcc ccagccagat cgagcgccat caacgacaaa cgctttatca
    26281 gttgatggac ggtctgaata cgttttacca acaatcttta cagcaacctg ttgccacgtc
    26341 tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgccc gctttgcgat
    26401 tggttttgcc ccccctggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa
    26461 tcgccagtca ttgattgatg cgggcatgtt ggtcactaac gatcaggggc gcagttacga
    26521 ccgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt
    26581 tggcgggcgt gtgctgggca acgatacacc caaatacctg aactcgccgg aaacggacat
    26641 tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc
    26701 caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat
    26761 taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt
    26821 ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc
    26881 ctggcgagcg ctggaaacgg cgctgcctta catgacagac ggtcgtcagc tacgctttat
    26941 gtttttgcct gatggcgaag accctgacac gctggtacga aaagaaggta aacaagcgtt
    27001 tgaagcgcgg atggagcagg cgatgccact ctccgcattt ctgtttaaca gtctgatgcc
    27061 gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccact
    27121 gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa
    27181 attaggcata cttgatgaca accagcttga acgattaatg ccaaaagcgg cagagagcgg
    27241 cgtttctcgc cctgttccac agctaaaacg cacgaccatg cgtatactta tagggttgct
    27301 ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa
    27361 gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctctc agccaggtct
    27421 gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa
    27481 actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc
    27541 actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga
    27601 gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct
    27661 ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc
    27721 gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac
    27781 aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc
    27841 tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt
    27901 cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg
    27961 ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg
    28021 ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc
    28081 gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc
    28141 gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg
    28201 acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa
    28261 tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt
    28321 gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa
    28381 gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac
    28441 gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc
    28501 atcgacccgg aactggctcg cgaaaaattt gcggaactgc gcgctcagta cgttgtaacg
    28561 cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa
    28621 ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac
    28681 agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc
    28741 gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc
    28801 agcgatacct ggttcaacgc ggcaattgca atgaacaagc cgtggtcgga aaaactgcac
    28861 gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc
    28921 ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa
    28981 gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc
    29041 aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg caacatcggt
    29101 ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc cacctacgca
    29161 acctggtgga tccgtcaggc gatcacccgc tctatcgcgg atcaggcgcg caccatccgt
    29221 attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg ccagatgctg
    29281 caagagatgg gccgtgaacc aacgccggaa gaactggctg aacgtatgct gatgccggaa
    29341 gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga aacgccgatc
    29401 ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct cgagctgccg
    29461 ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg
    29521 accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa caccgaccac
    29581 acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg tcagatcgaa
    29641 gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgctgcg tagcttcctg
    29701 gacgattaat cggtaggccg gatcaggtgt tacgccgcac ccggcactag tccctccgca
    29761 caaacgccac cttttcggtg gcgcgaaaat tgatgtaatg caatgaaaaa taaaggatta
    29821 tttttctcgt tgtccacatt ttgccatagc gatacatctt agcccgtaat gattcgcggg
    29881 ctaagcatat acaaaatgtt aagcctgatg ttggaaaaag tttgtttttc ctttgtgtag
    29941 gaaagaacaa agggtggcgg aaagttcaga aagccagcga ttatggcgta attattctga
    30001 cagaagaaca atttatcaat tttattgata ctggtgaaat tcctcttgag caagactgcg
    30061 tggttgagaa tgatatcagt aaatcgcttg agaaggatgt taacacttac aagagtatga
    30121 tagatgagtt ctcgctttct atccgtactg taagggagtt tcgccgtagt aatgcgttga
    30181 ttgcggtatt tgttgatggt tacgcagctg gttggcgctt tgctgtaaaa gagtgttaca
    30241 aggattcttt ggatataaaa cttaccccag taaaaacaga gttttttaat tatgaaattt
    30301 ggacgcaagg ggattccttt cagtttaatc gcggtgatgt tttttatagt gatcgcctag
    30361 cgtatgattc ttattgcgat tttttaaagc ttgatgatgc tgtcgttctt caggtga
//
LOCUS       DAZGHF010000050        30018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGHF010000050 DAZGHF010000000
VERSION     DAZGHF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.050"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            274..420
                     /locus_tag="ACHK3A_002346"
     CDS             274..420
                     /locus_tag="ACHK3A_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740412.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            428..682
                     /gene="xisR"
                     /locus_tag="ACHK3A_002347"
     CDS             428..682
                     /gene="xisR"
                     /locus_tag="ACHK3A_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO8740413.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            716..2005
                     /locus_tag="ACHK3A_002348"
     CDS             716..2005
                     /locus_tag="ACHK3A_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8740414.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            2050..2700
                     /gene="rcsB"
                     /locus_tag="ACHK3A_002349"
     CDS             2050..2700
                     /gene="rcsB"
                     /locus_tag="ACHK3A_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO8740415.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(2786..5635)
                     /gene="rcsC"
                     /locus_tag="ACHK3A_002350"
     CDS             complement(2786..5635)
                     /gene="rcsC"
                     /locus_tag="ACHK3A_002350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO8740416.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPLTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            5802..7628
                     /gene="atoS"
                     /locus_tag="ACHK3A_002351"
     CDS             5802..7628
                     /gene="atoS"
                     /locus_tag="ACHK3A_002351"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFO8740417.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            7625..9010
                     /gene="atoC"
                     /locus_tag="ACHK3A_002352"
     CDS             7625..9010
                     /gene="atoC"
                     /locus_tag="ACHK3A_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFO8740418.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSNETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            9206..9868
                     /gene="atoD"
                     /locus_tag="ACHK3A_002353"
     CDS             9206..9868
                     /gene="atoD"
                     /locus_tag="ACHK3A_002353"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFO8740419.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            9868..10518
                     /gene="atoA"
                     /locus_tag="ACHK3A_002354"
     CDS             9868..10518
                     /gene="atoA"
                     /locus_tag="ACHK3A_002354"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFO8740420.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            10515..11837
                     /gene="atoE"
                     /locus_tag="ACHK3A_002355"
     CDS             10515..11837
                     /gene="atoE"
                     /locus_tag="ACHK3A_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFO8740421.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPIMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            11868..13052
                     /gene="atoB"
                     /locus_tag="ACHK3A_002356"
     CDS             11868..13052
                     /gene="atoB"
                     /locus_tag="ACHK3A_002356"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFO8740422.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATKKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(13126..13902)
                     /gene="yfaP"
                     /locus_tag="ACHK3A_002357"
     CDS             complement(13126..13902)
                     /gene="yfaP"
                     /locus_tag="ACHK3A_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO8740423.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(13907..15556)
                     /gene="yfaQ"
                     /locus_tag="ACHK3A_002358"
     CDS             complement(13907..15556)
                     /gene="yfaQ"
                     /locus_tag="ACHK3A_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO8740424.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVNSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVLVELFARYPLKKITAEKSS
                     TAVKPGVLNGRYRVTFANGNHIMFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTFQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(15557..20071)
                     /locus_tag="ACHK3A_002359"
     CDS             complement(15557..20071)
                     /locus_tag="ACHK3A_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO8740425.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDEDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARIPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTEKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIQLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPDYWQHMLDSYAEQADNMPILHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGKLAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(20095..20718)
                     /gene="yfaT"
                     /locus_tag="ACHK3A_002360"
     CDS             complement(20095..20718)
                     /gene="yfaT"
                     /locus_tag="ACHK3A_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO8740426.1"
                     /translation="MRHGLLALICWLCCVVTHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(20715..22403)
                     /gene="yfaA"
                     /locus_tag="ACHK3A_002361"
     CDS             complement(20715..22403)
                     /gene="yfaA"
                     /locus_tag="ACHK3A_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO8740427.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGSAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKAQAVQPDQLMSDYFFRVSLA
                     MQNKTMLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(22552..25179)
                     /gene="gyrA"
                     /locus_tag="ACHK3A_002362"
     CDS             complement(22552..25179)
                     /gene="gyrA"
                     /locus_tag="ACHK3A_002362"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO8740428.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLIIQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            25326..26048
                     /gene="ubiG"
                     /locus_tag="ACHK3A_002363"
     CDS             25326..26048
                     /gene="ubiG"
                     /locus_tag="ACHK3A_002363"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO8740429.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVEYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(26188..29946)
                     /gene="yfaL"
                     /locus_tag="ACHK3A_002364"
     CDS             complement(26188..29946)
                     /gene="yfaL"
                     /locus_tag="ACHK3A_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO8740430.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSGNGAAAVIDSCQGYDIKANCQ
                     ASRQSLSGNMQDWSIADGQWLIFSGMANNASGGAVFLQQSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEISIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLESVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQVLDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAISLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTAVV
                     INPITGIGEPTSTGIKVVDFAADPTQFQNNAQFRLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVTPTPPPDPDPTPDPDPTPDPDPTPDPGPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDQAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGVWLDSWLQ
                     YAWFNNDVSEQDDGEDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQTRLGLHSEWSTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     GVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         7300 a         8056 c         7355 g         7307 t
ORIGIN      
        1 ggctcagtga atgggtgaaa tggttgatat taacgatgaa ctggtccgca cagtaattac
       61 cgttgatgac ggatgcgacc atacccagcg ttttatctgg atgatgaata cacggcgtaa
      121 tatcagaaac aaagtgttcc ggcacgaacc aaagccagtc agggtgacaa agatacgcat
      181 tgaagccaga aaacgccgca aatacaggcc acggaaggcc agggccccag cgcaaccgaa
      241 tgaactggca taaagacagc aggagaataa gtcgtgaatc gcgagtttga aatatggata
      301 cgactcagat acgggtaccg atacgattta accagagatg ctcacggctt ctactgccgt
      361 gaagttgtaa agcgaatgtt tgacgtgtgg tgccactgcc gtggcctgaa gatagtgtga
      421 gtcaggaatg gaaacaatca tctatcagat aacgcccagc aaatggtgca cagaaagaac
      481 ccttattgct gcaactggac tgaaatcagg aaccattgcc agagcaagaa aaaaatcctg
      541 gttactgggg aaagaatacc gtcattacgc agcagaagga gagcccgggc ctttcagtga
      601 atgtgtatac aacattgagg aaattatgcg ctggatcgaa aaccagaaac aaccaggtgc
      661 caaaaatgca agttccggtt aacctgttaa tgctcctgga cgtctgggag gtttaatgag
      721 taacgcatca tacccgacag gcgttgaaaa ccatggcgga tcactccgca tatggtttca
      781 ctataacggc aaacgtgtca gagaaaacct cggtgttcct gacaccgcca aaaaccggaa
      841 gatcgcaggt gaacttcgca cttccgtttg ttttgcaata agaatgggga gcttcgacta
      901 cgccgcgcag ttccctaatt cccctaacct gaaacatttt ggtctgggaa aaaaagagat
      961 aaccattaag gcactttcgg aaaaatggct tgagcttaag aaaatggaaa taacagcaaa
     1021 tgccatggtc agatacaaat cagctatccg aaattgcctt gactgccttg gagggaacaa
     1081 actggcatca tcaattacac aggaagatgt tctcattttt cgtaaagaat tattaacggg
     1141 atatcagttg ccaggaaaaa atcagaggaa gcctattaaa ggtcgtagtg tacctactgt
     1201 aaatagctat atgacaatta tctccgggat gttccgtttt gcggcaaaca atgggtatat
     1261 aggcaaaaac cctttcagtg agcttccatc actgaaaaga gcgaaaacac ctcccgatcc
     1321 actaacaaaa gatgagtttg ccagactgat tgatgcgtgc caccaccagc aaactaaaaa
     1381 tttctggtcg ctggctgtat ataccgggat gcggcatggc gaactgtgtg ggctggcatg
     1441 ggaggatatc gatctggaag cgggtacctt gaccgtcaaa aggaattaca ctcagacaaa
     1501 ggaatttaca ctacccaaaa cagatgcagg aacaaacagg gtaatacaac tcatacagcc
     1561 agcaattgac gtattaaaaa atcaggcgac attaaccagg cttggtaaac aatatcagat
     1621 agaagtaaag ctgcgagagt atggccggac aagcactcac tcatgcagct ttgttttcaa
     1681 tcctcagata ttagatcgca caggtaactc aggagttcat tatgccgtaa catccatcaa
     1741 cagaacatgg gaatctgcat taaaacgagc aaaaattcgc catagaaaag cgtatcagtc
     1801 tcggcatact tatgcttgct gggctttaac tgccggagca aatccaaact tcatagccaa
     1861 ccagatggga catgcgaatg cccagatggt ttacaatgtt tacggtgcat ggatgaaaga
     1921 caataaccag tcccaggtag aagtactaaa cagaaagcta tgtacttatg tcccacaggt
     1981 gccccaacga ctaaacaaag catagaatat atttttaaaa tcaaatagtt aatacatagg
     2041 tgcaagtaca tgaacaatat gaacgtaatt attgccgatg accatccgat agtcttgttc
     2101 ggtattcgca aatcacttga gcaaattgag tgggtgaatg ttgtcggcga atttgaagac
     2161 tctacagcac tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat taccgatctc
     2221 tccatgcctg gcgataagta cggcgatggc attaccttaa tcaagtacat caagcgccat
     2281 ttcccaagcc tgtcgatcat tgttctgact atgaacaaca acccggcgat tcttagtgcg
     2341 gtattggatc tggatatcga agggatcgtg ctgaaacaag gtgcaccgac cgatctgccg
     2401 aaagctctcg ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt ttctcgcctg
     2461 ttggaaaaaa tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa agagagtgaa
     2521 gttctacgcc tgtttgcgga aggtttcctg gtgaccgaga tcgctaaaaa gctgaaccgc
     2581 agtatcaaaa ccatcagtag ccagaagaaa tctgcgatga tgaagctggg tgtcgagaac
     2641 gatattgctc tgctgaatta tctctcttct gtgacgttaa gtccggcaga taaagactaa
     2701 tcacctgtag gccagataag acgcgtcagt gtcttatctg gcatttgcac ggattgccgg
     2761 atgcggcgta aacgccttat ccgtcctacg attcccgcga tttcctgacc ctctcggcat
     2821 ataccgtcag cgtctgtttt atcacatcca gcgttaccgg cttcgacagg cagctgtcca
     2881 taccggactc cagacaccgc tgcttctctt cagccaacgc attagcagtt actccgatta
     2941 ccggcaacgt cagtcccaac tgacgaatgc gttgcgtcaa gcggtaacca tccatatttg
     3001 gcatgttgac gtcgctaagc acgatatcaa tatgattctt gctaagtaca ttaagcgcat
     3061 cgacgccatc attcgcggtt ttacattgat agcccaacga tcccaactga tctgccagca
     3121 gacgccggtt aatcggatga tcatccacga ccagaatcat catatcgtca ttatcgctga
     3181 ccgctttgtc cgttaacggc agagcgttag caggatcgtc gctctccatc tcgatcaaat
     3241 aaatacgcgc caacaatgcc ggtagctcat gcggggcagc cacactgtgt acccactctc
     3301 ctggcgcttt ctccagcgga ataccaatat ggcgacgaca gaaggtcaca accgctctac
     3361 cctgccattt tttgctcact acctcgtcag tgattaacac atcttcggga gtcggttcct
     3421 gcccttcgta tgttgtaacg acgatgccgc tgcgctgcaa actggtttcc aggaactgac
     3481 agagcgacgc attgcggacc gccagccagc aacgtttacc actcaaccct tccacgcctt
     3541 ttttctgcgg gtactgagcg ccgtacaacg gaatacgcac ggtaaactgg ctgcccattc
     3601 ccggttctga atctaccgag atgtcgccgt ccatcatgct gatcagtttt tcacaaatcg
     3661 ccagacccag accggtcccc tggaagttac gctgtacgcc cgttccgacc tggaagaagg
     3721 gatcaaacaa gcgcaccact tctttcgccg gaatccctac gccggtatcg cgaacacgga
     3781 tagagagata atcgccatca gcgcgaacat gcagaactat acagccggta tcggtgaatt
     3841 ttatggcgtt actcaacagg ttggagatga cctgctgtaa acgcatcggg tcgccattta
     3901 aggccactgg cacatccggt tcaataaagc agtacaagcc tagctgcttg cgtaccacca
     3961 gcggtaaata gttggcggtg atgtggttca tcacttcacg cggtgaaaac tcacgcggtt
     4021 cgatcttcaa ctgttccgat tcaatcttcg agaagtcgag aatatcgctg ataattttca
     4081 acaacaggct ggaagagttg ttcattgccg tcaccagacg atcgacgcct ttcggtaact
     4141 ctttggtttg caacagatcc aggttaccga taatgccata cagcggcgtt cgcagctcat
     4201 gactgacggt ggcaaggaac atcgattttg actggctcgc ctgttccgct gcttgtgcca
     4261 tctcctgcaa cgactcttcc atcttcacgc gcgaagaaac atccaccagc acacaaatgg
     4321 ccacgttttc attacgatag cgcgaatgga cgaagctgat ttgcaggttg gtattgttgc
     4381 tggtcaggac atcaacaaaa ttgacctgct gcccacagat aatttgtgtc agccgttggc
     4441 ggtcctcatg cgtaagcata ttgagatagg tatgcgccag ttcgttactt aaaatattga
     4501 cgccatcagc ggtacgcaaa atgcagatac ccactggcgc ggaggcgaca atcttgcgat
     4561 taaactgctc atgttcttcc agtcgcaggg cgtcgctttc cgccggaatg aaaatacgcc
     4621 gctcgtacat ccgcgcgaga gtaaacaatg cagctccggc aagcacattc agcaaaattg
     4681 cgttaaggat caacatgcga atgcgttcca gcaccttatc aaccggcacc gaatacacga
     4741 tgcttagcga tgagggtggc agatttttct tcagcaccag ctcccggaac ccttccgtat
     4801 agccaaacca ggagcgttcc tgcatccagc gaggatcgcc cttaattttg ctttctggtc
     4861 cggtaagcga aatcagggta tgaccatttt catcaagaat ggtaaccccc atcggcaacg
     4921 taccaggtaa gaaaaagttc tccatccgga tggtctgctc gacacccaaa agcgcctgca
     4981 accggttcgc cagataaact ggcgtcaagg cgtaaaaata cccgacgcca gggcgcggac
     5041 cttcgctgat ccagtagagg ttactgccac tatcatcttg tggtgcattt cgatatttat
     5101 taatgcgttc atgcaaagct tttaacgcgg tatcgcgttc cactggcata tcacgcagac
     5161 cgaaattggc catgcagagg ttatcgctgc cgattaaaaa tacccggttg agatcgtaag
     5221 ccgcagaaaa attatcgcgc cagtagcgca taaaccacgc caatgactcc agagaacctc
     5281 gccaggtgtt actcattgcg gaacaatcag agtcagcaaa tagcggttca aacgcaggca
     5341 catccgtctg cgtttctcgt ccacgcgggg aaagcacacc gttttctgcc gataagcgat
     5401 tttcagctat gtacttcagc tctttcatca catcagaggt gcgttgaata aagcgctgag
     5461 cctgatcgga actcagatta aattcctgac gaatttccga ttctcgctga tgtaacgcat
     5521 taacgatgta aaaaacggat gaaaaagcaa tcaacagcca gagcactaac gccaatgctc
     5581 tgaacatgta gcgcgaggct ttcagggttg tacgaaaaga agcaaggtat ttcaaagggg
     5641 cgaagctccg cctcaggtga ccgatggagt gtggttaagg tagcggtaaa agcgtgttac
     5701 cgcaatgttc tctcttctct ggaatatgat acaccgccga gaaatcatca ccttaacctc
     5761 tgataatcgt catataccgg acaagactag tggatttcag catgcattat atgaagtgga
     5821 tttatccacg ccgcttacgt aatcaaatga tcctgatggc aatcctgatg gtcattgtcc
     5881 caacgcttac tattggttat atcgtagaaa cggaaggacg ttcagcagtc ttatctgaaa
     5941 aagagaaaaa actttctgcc gtggtcaacc tgcttaatca ggcgctaggc aatcgctacg
     6001 atctctacat cgacttaccg cgtgaggagc gtatccgcgc attaaatgca gaacttgccc
     6061 ccattaccga aaatatcact cacgccttcc ctggcatcgg cgctggttat tacaacaaaa
     6121 cgctggatgc gataatcacc tacgcgcctt cagcgctata tcagaataat gtgggcgtta
     6181 ccattgccgc agatcaccct ggtcgcgaag tcatgcgtac aaatacccct ttggtttatt
     6241 caggcagaca ggtgcgcggc gatattttga attcaatgat tcccattgag cgtaatggtg
     6301 aaatcctcgg ctatatctgg gccaatgaat taaccgaaga tattcgccgc caggcctgga
     6361 aaatggatgt gaggattatc attgtgctca ccgccggttt gctgataagc ctgctgttga
     6421 ttgtcctttt ctcccgtcgc ctgagcgcca atatagatat catcaccgat ggcctctcga
     6481 ctctggctca aaatattccc actcgattac cacaattgcc cggtgaaatg gggcaaatca
     6541 gtcagagtgt taacaacctc gcccaggcac tgcgtgaaac gcgaacactt aacgatctga
     6601 ttattgaaaa cgctgccgat ggcgtcattg ccattgaccg ccagggtgat gtaaccacca
     6661 tgaacccagc agcagaagtc atcactggct atcaacgtca tgaactggta gggcagcctt
     6721 actccatgtt gttcgacaat actcagttct acagtcccgt actggatacg ctggaacatg
     6781 gcaccgaaca tgtagcactg gagatcagtt ttccaggccg tgaccgcacc attgaactca
     6841 gtgtcacgac cagtcgtatt cataacacgc acggtgaaat gataggtgct ttggtgattt
     6901 tctctgattt aactgcccgc aaagaaaccc agcgccgcat ggcgcaagca gaacgcctcg
     6961 ccacactggg tgagctgatg gctggcgtgg cgcatgaagt acgtaatccg ctaacagcta
     7021 ttcgtggtta tgtacagatc ttgcgccaac aaacccgtga cccaatacat caggaatatc
     7081 tgtccgtagt actcaaagaa atcgattcaa ttaacaaagt tattcagcaa ttgctcgaat
     7141 tttcacgccc acgccacagt caatggcaac aagtcagcct caatgcattg gttgaagaaa
     7201 ctctggtact ggtacaaacc gccggcgtac aagcgcgggt cgacttcata agcgaactgg
     7261 ataatgaatt aagcccgatt aacgccgatc gtgaactgct caaacaggta ctactgaata
     7321 tcctgatcaa tgccgtccag gctatcagtg cacgagggaa aattcgcatt cgaacctggc
     7381 aatacagcga ctcacaacag gccatttcga tagaggacaa cggctgtggc attgatctct
     7441 cgctgcaaaa aaagatcttc gatccctttt tcaccaccaa agcctcagga accgggcttg
     7501 gtctggcgtt aagtcaacgc atcattaatg cccatcaggg tgatattcgc gtcgccagtt
     7561 tgccgggcta cggcgcaacc ttcacgctta ttttaccgat caacccgcag ggaaatcaga
     7621 ctgtatgact gctattaatc gcatccttat tgtggatgat gaagataatg ttcgccgtat
     7681 gctgagcacc gcttttgcac tacaaggttt cgaaacacat tgtgcgaaca acggacgcac
     7741 agcattacac ctgtttgctg atattcaccc tgacgtggtg ttgatggata tccgcatgcc
     7801 agagatggac ggcatcaagg cactaaagga gatgcgcagt aatgagaccc ggacacccgt
     7861 tattctgatg acggcctatg ctgaagtgga aaccgccgtc gaagcgctac gctgcggggc
     7921 cttcgactat gttattaaac cgtttgatct cgatgagttg aatttaatcg ttcagcgcgc
     7981 tttacaactc cagtcaatga aaaaagagat ccgtcatctg caccaggcac tgagcaccag
     8041 ctggcaatgg gggcacattc tcaccaacag cccggcgatg atggatatct gcaaagacac
     8101 cgccaaaatt gccctttctc aggccagcgt cttgattagc ggtgaaagcg gcaccgggaa
     8161 agagttgatt gccagagcga ttcactacaa ttcgcggcgg gcaaaggggc cgttcattaa
     8221 agtcaactgc gcggcgctgc cggaatcgtt gctcgaaagt gaactgtttg gtcatgaaaa
     8281 aggtgcattt actggtgcac aaaccttgcg tcagggatta tttgaacgtg ccaacgaagg
     8341 tactctgctc ctcgacgaaa ttggcgaaat gccgctcgta ctgcaagcca aattactacg
     8401 cattctgcag gaacgggaat ttgaacggat tggcggtcat cagaccataa aagttgatat
     8461 ccgcatcatt gctgccacca accgtgactt gcaggcaatg gtaaaagaag gcaccttccg
     8521 tgaagatctc ttttatcgcc ttaacgttat tcatttaata ctgccgcctc tgcgcgatcg
     8581 ccgggaagat atttccctgt tagctaatca ctttttgcaa aaattcagta gtgagaatca
     8641 gcgcgatatt atcgacatcg atccgatggc aatgtcgctg cttaccgcct ggtcatggcc
     8701 gggtaatatt cgagagcttt ccaacgtcat tgaacgcgcc gtcgtgatga actcaggccc
     8761 gatcattttc tctgaggatc ttccgccgca gattcgtcag ccagtctgta atgctggtga
     8821 ggcaaaaaca gcccctgtcg gtgagcgtaa tttaaaagag gaaattaaac gcgtcgaaaa
     8881 acgcatcatt atggaagtgc tggaacaaca agaaggaaac cgaacccgca ctgcgttaat
     8941 gctgggcatc agtcgccgtg cattgatgta taaactccag gaatacggta tcgatccggc
     9001 ggatgtataa cgccaaaact tgctatgcag aaatttgcac agtgcgcaat tttctgcata
     9061 gccgctcatt ctccttatga atccccatct aatttattcc tttctattca attagttaaa
     9121 taacaacatt caataatctc attctggcac tccccttgct attccctgac tgtacccaca
     9181 acggtgtatg caagagggat aaaaaatgaa aacaaaattg atgacattac aagacgccac
     9241 cggcttcttt cgtgacggca tgaccatcat ggtgggcgga tttatgggga ttggcactcc
     9301 atcccgcctg gttgaagcat tactggaatc tggtgttcgc gacctgacat tgatagccaa
     9361 tgataccgcg tttgttgata ccggcatcgg tccgctcatc gtcaatggtc gagtccgcaa
     9421 agtgattgct tcacatatcg gcaccaaccc ggaaacaggt cggcgcatga tatctggtga
     9481 gatggacgtc gttctggtgc cgcaaggtac gctaatcgag caaattcgct gtggcggagc
     9541 tggacttggt ggttttctca ccccaactgg tgtcggcacc gtcgtagagg aaggcaaaca
     9601 gacactgaca ctcgacggta aaacctggct gctcgaacgc ccgctgcgcg ccgacctggc
     9661 gctaattcgc gctcatcgtt gcgacacact tggcaacctt acctatcaac ttagcgcccg
     9721 caactttaac cccctgatag cccttgcggc tgatatcacg ctggtagagc cagatgaact
     9781 ggtcgaaacc ggcgagctgc aacctgacca tattgtcacc cctggtgccg ttatcgacca
     9841 catcatcgtt tcacaggaga gcaaataatg gatgcgaaac aacgtattgc gcgccgtgtg
     9901 gcgcaagagc ttcgtgatgg tgacatcgtt aacttaggga tcggtttacc cacaatggtc
     9961 gccaattatt taccggaggg tattcatatc actctgcaat cggaaaacgg cttcctcggt
    10021 ttaggcccgg tcacgacagc gcatccagat ctggtgaacg ctggcggaca accgtgcggc
    10081 attttacccg gcgcagccat gtttgatagc gccatgtcat ttgcgctaat ccgcggcggt
    10141 catattgatg cctgcgtgct cggcggtttg caagtagacg aagaagcaaa cctcgcgaac
    10201 tgggtagtgc ctgggaaaat ggtgcccggt atgggtggcg cgatggatct ggtgaccggg
    10261 tcacgcaaag tgatcatcgc catggaacat tgcgccaaag atggttcagc aaaaattttg
    10321 cgccgctgca ccatgccact cactgcgcaa catgcggtgc atatgctggt tactgaactg
    10381 gctgtctttc gttttattga cggcaaaatg tggctcaccg aaattgccga cgggtgtgat
    10441 ttagccaccg tgcgtgccaa aacagaagct cggtttgaag ttgccgccga tctgaatacg
    10501 caacggggtg atttatgatt ggtcgcatat cgcgttttat gacgcgtttt gtcagccggt
    10561 ggcttcccga tccactgatc tttgccatgt tgctgacatt gctaacattc gtgatcgcgc
    10621 tttggttaac accacaaacg ccgatcagca tggtgaaaat gtggggtgat ggtttctgga
    10681 acttgctggc gtttggtatg cagatggcgc ttatcatcgt taccggtcat gcccttgcca
    10741 gctccgctcc ggtgaaaagt ttgctgcgta ctgccgcctc cgccgcaaag acgcccgtac
    10801 agggcgtcat gctggttact ttcttcggtt cagtcgcttg tgtcatcaac tggggatttg
    10861 gtttggttgt cggtgcaatg ttcgcccgtg aagtcgcccg gcgagtaccc ggttctgatt
    10921 atccgttgct cattgcctgc gcctacattg gttttctcac ctggggtggc ggtttctctg
    10981 gctcaatgcc tctgttggct gcaacaccgg gcaacccggt tgagcatatc gccgggctga
    11041 tcccggtggg cgatactctg ttcagtggtt ttaatatttt catcactgtg gcgttgattg
    11101 tggtgatgcc atttatcacc cgcatgatga tgccaaaacc gtctgacgtg gtgagtatcg
    11161 atccgaaact actcatggaa gaggctgatt ttcaaaagca gctaccgaaa gatgccccac
    11221 catccgagcg actggaagaa agccgcattc tgacgttgat catcggcgca ctcggtatcg
    11281 cttaccttgc gatgtacttc agcgaacatg gcttcaacat caccatcaat accgtcaacc
    11341 tgatgtttat gattgcgggt ctgctgctac ataaaacgcc aatggcttat atgcgtgcta
    11401 tcagcgcggc agcacgcagt actgccggta ttctggtgca attccccttc tacgctggga
    11461 tccaactgat gatggagcat tccggtctgg gcggactcat taccgaattc ttcatcaatg
    11521 ttgcgaacaa agacaccttc ccgatcatga ccttttttag ttctgcactg attaacttcg
    11581 ccgttccgtc tggcggcggg cactgggtta ttcaggggcc gttcgtgatg cccgcagccc
    11641 aggcgctggg cgctgatctc ggtaaatcgg taatggcgat cgcctacggc gagcaatgga
    11701 tgaacatggc acaaccgttc tgggcgctgc cagcactggc aatcgccgga ctcggtgtcc
    11761 gcgacatcat gggctattgc atcactgcct tgctcttctc cggcgtcatt ttcgtcattg
    11821 gtttaacgct gttctgacgg caaccctaca gacagaagga atataaaatg aaaaattgtg
    11881 tcatcgtcag tgcggtacgt actgctatcg gtagttttaa cggttcactc gcttccacca
    11941 gcgccatcga cctgggggcg acagtaatta aagccgccat tgaacgtgca aaaatcgatt
    12001 cactacacgt tgatgaagtg attatgggta acgtgttgca agccggactg gggcaaaatc
    12061 cggcacgtca ggcgctgcta aaaagcggac tggctgaaac ggtgtgcgga ttcacggtca
    12121 ataaagtgtg tggttcgggt cttaaaagtg tggcgcttgc cgcccaggca attcaggcag
    12181 gtcaggcgca gagcattgtg gcggggggta tggaaaatat gagtttagcg ccctacttac
    12241 tcgatgcaaa agcacgctct ggttatcgtc ttggagacgg acaggtttat gacgtaatcc
    12301 tgcgcgatgg tctgatgtgc gccacccatg gttatcatat ggggattacc gccgaaaacg
    12361 tggctaaaga gtacggaatt acccgtgaaa tgcaggatga actggcgcta cattcacagc
    12421 gtaaagcggc agccgcaatt gagtccggtg cttttacagc cgaaatcgtc ccggtaaatg
    12481 ttgtcactcg gaagaaaacc ttcgtcttca gtcaagacga attcccgaaa gcggattcta
    12541 cgactgaagc gttaggcgca ttgcgcccgg ccttcgataa agcaggaaca gtcaccgccg
    12601 ggaacgcgtc tggtattaac gacggtgctg ccgctctggt gattatggaa gaatctgcgg
    12661 cgctggcagc aggccttacc cccctggctc gcattaaaag ttatgccagc ggtggcgtgc
    12721 cccccgcatt gatgggtatg gggccagtac ctgccacgaa aaaagcgtta caactggcgg
    12781 ggctgcaact ggcggatatt gatctcattg aggctaatga agcatttgct gcacagttcc
    12841 ttgccgttgg gaaaaccctg ggctttgatt ctgagaaagt gaatgtcaac ggcggggcca
    12901 tcgcgctcgg gcatcctatc ggtgccagtg gtgctcgtat tctggtcaca ctattacatg
    12961 ccatgcaggc acgcgataaa acgctggggc tggcaacact atgcattggt ggcggccagg
    13021 gaattgcgat ggtgattgaa cggttgaatt aatcaataaa aacacccgat agcgaaagtt
    13081 atcgggtgtt ttcttgaaca tcgacggcga aggtaacccc attaatcacc agtcaaaact
    13141 tttcaccagc gtcagctcgc cagcattacg catcggtaca ataaatgttt cctgtttctc
    13201 attgaccgat ccttcatcgg tgatcagcgt cagttgggcg gtcgtcaatt ccgtttcgct
    13261 gcgtccgcca tagtagttga tatacacctg ataacgtccg tgaatcggcg ctggcatggc
    13321 gaaaatctcg ggtccgtacc ccgtcgtgac atccatatcc agtgcaccac tgtttttcag
    13381 caccgtgtta ccgtaccagg cgtgttcgcc atcgggcgtg acaacatgga gatcgagatc
    13441 ggtattgtcc gtatcccacg agagaaccag ccgtaaacgt gcacgaatcg ttcctgtacc
    13501 cggcgttgag taaaactgca ttttttgccg gctttgccca tccgggctga tgacctgcac
    13561 gctgttgctg ccttccgtga aaatataagg gcgtgcaaat gaaccatcgg attcaattct
    13621 ctgcggcata ctggcaccgt tgaccaccag ccggccctgc tgaactttac ccgccgcagc
    13681 ataattcttt attttgccgc ggatctgcgc tgagatattt tgatcatctg ccatgttgac
    13741 ggacgacgcc gggtaattga tcgattgtga aaaactggca tcttcaccct ctgccggatg
    13801 ccagccagag agcggtgcat caatctcgac ttcctgcaca ccttcactgt gagcgacagg
    13861 cgaaagcgcc accagtaaca acggaagaaa aatctttcgc atagcattag tccattaata
    13921 attgtcgggt aagcgtttcg atatagttct catcaagccc ggtgggataa ccatcaaagg
    13981 ccaggtgcag atactcatgg gttaaatcca gccgatcctg aaacgtaaag aagttacgaa
    14041 taaacagccg tttctgctgc cgatcggtat aggggaaacc tgagactaaa cggcaaaccg
    14101 caaacacgtc tggttcgttg taccccgtct caccttgtaa tatacgccgc cactgcggca
    14161 tttttttcgc cagccaggct ttcgctttgg gtaataactg gcaggttgag cgtggcgctc
    14221 cccagcggct aaggctattg tcgggataag caaacgcgag tatctgatcg taacgctctc
    14281 cctgccccgc ctgggccgtg gcttgccgcc aggaaagcgt cccttcggta gcgcgactgc
    14341 cgtgataatg aaccggatcg cccgcataga taagatcctg cgtccaggcg gtcatcgttc
    14401 gcgcccccgt ggtagctggc gaagcagaaa cacgctgcgt ggcgctgctg tcagggatgg
    14461 tcaggcaatc gccttcccga ttggcgtttt gctgtaaaaa agtgcgtatc gccacggtca
    14521 tggctttcgc tgcctcaggt ggcgtacttt tcgcctcacg atccagaact ctggcaacgt
    14581 actcttcacg atcgagatgc gactgcaatt tcagtttgcc cttttcgctc agtaaggtcg
    14641 tctcaccgtg gctgacaaac ataatgtggt taccattggc aaacgtcaca cgatagcgcc
    14701 cattaagtac gccgggtttt actgccgtcg aactcttttc cgccgtgatt ttcttcagcg
    14761 gatagcgggc aaacagctcc accaggacac attgcccact ggcaacttgc gtcggcactg
    14821 gcaaaacccg attaagcacc gtcgcataac gggtcagtac cgttttgctg gtgccactgc
    14881 cagtcaccca cagcggtgta ccatcggtta accagccggc aaaaccaccc tgacggattt
    14941 cctgtttatc atcggcaaac cagctccagg ttttcacccg tacccgactg ccgagcatgg
    15001 atgcgacacc gattttcgcc tcgtccagca ccacatccag taagacttcc tgagctttgt
    15061 tttgcgcagg taacgtcgct aatgaatcaa gcagcgagtt aaccgttacc gaggtttctg
    15121 gcttcagcgt agtaagagac gctaaccacg caggtgcctg acgctgttgc caatattgcc
    15181 cccacacatc tgcgccaata tgcaaccgct gcggcgcaaa atagagccca caggaacgta
    15241 ccagcgcggt atcgcgcgtt atactctccc cagcctgaca acaataaacc tcttccgggg
    15301 agtttccgcg acactggtag ggctgctccg gttgatgggt atcctccagc caggcgtaaa
    15361 catacagttt ccacaaactc cctaacggcg tggtgagcgt gtcaggtaat gccgagactt
    15421 tggtcacgcc tgatgacgac agctgataca gttgatcgtg ctgcgcaccg cgcagcacca
    15481 gttgtaacgg cgtctcctcc gcaagcgttg gcagagtaac caacgccaat agccagacga
    15541 ttctgcgcca gttcacttat ttcacctgca tcccggtcca ttcgctccct gccgcaacac
    15601 tttgctgtgc aggtgcatag gaacgcacat aacgagcagg aggcagaacg aattgccctt
    15661 tttgcgagaa gcgcagcaaa tggcggaaag tgaccgttcc cgtcagttct ttcaccggca
    15721 ccatataggc cagtttgccc atttcatttc gcgctttttc cagcaactgc ccctgctgtt
    15781 ttccagcgtt cggtttattg accgaaatgc cccacgtagt cctctcaacg tcagcccctg
    15841 gcgggagcgg tacttccacc tgaccgtagc gcagaacggc atcctgctcg ctggtaagcg
    15901 taatttcatc gagatacagc gcatcgctgt caatctcatt gctggtcacc ggctgcagag
    15961 taaagctcat ctcttcttca ccggggataa gccgatacaa ctggcgttca acggtcaccg
    16021 gaatgttact ttgttgagcc gtttttgccg gttcacgcca gcggacctgc acattttgcg
    16081 gcgataattc gtcaccaaaa gagagaatgt ccggcacacc ctgaccaacc caacgccagt
    16141 cttcgccccc tccagttaac ttatgtttag cccatgcgcc cgcaggcgca ggcaaaacaa
    16201 ctggaggcat tgtcgccata tatttcgcca gccagttcat ggccagcgcg cgctcaatag
    16261 tggattgctc agcggtcaaa ccacttaaaa tagcggccgc ctgggtagcg tcaccaccgc
    16321 tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct ttatcccacg
    16381 catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc agcaacgtca
    16441 tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg aggatcaggc
    16501 tgtcattgat atcgctggtg tcttcctcag agaaatcttc agttttagtt ccgcgccggg
    16561 cgatagcttc atccaaccct ttcaacaacg ttttgcacgg tagattcatc tcctgtgccc
    16621 acgccagcac cagcgcgcga tgcaatatcg gcatgttatc tgcctgctcg gcataactgt
    16681 cgagcatatg ttgccagtaa tccggttgct gcgttacgcc gagcgcctgg ctggcctgcc
    16741 agtcggcgta ccatgcccat gccgtcagga aggcgtcgcc attgccatct tcaccccacc
    16801 aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta tcctgaatca
    16861 tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagtgaacgc caggcgagac
    16921 tgagcgggat cagacggcta ccggtgttga tcacgccccc ccacggttca tcgactaacg
    16981 catcaagatt gttacggaaa atctcctgcg gcgtttcgct actttgtagc tggatattgc
    17041 tcgcctgctc gggcaacatc agcgcgttat cgccaccgcc gagcatgacg tttttctgct
    17101 gttcaacggg ccagctgtta tccacaaaag acagttttgt gctaatgctg tcctgtactt
    17161 gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc tgcgtcagcg
    17221 aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagca cctgcaaatt
    17281 tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt cctgctgccg
    17341 gtttgtcgcc catgcgatac accgttggca tactccactt catgtagaga tttttttccg
    17401 aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca cgcgcggtga
    17461 tgcgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg ccttgtttat
    17521 cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt ggacgttcaa
    17581 gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc tcgctggaga
    17641 gcgcctggtc gtagctgata aacgacagac tggagctggt acgcacattg ttacgcccca
    17701 gcggatagaa aaatttgccg atattcggcg cgatttccgg ttgcagcgcg tagatcattt
    17761 catcgaccac accaaccgtt agctgcgcag aaacaggttt acctttcagc gacgaggtta
    17821 attcgacatt gaccagttca ccaggctggt aatgggtttt ttccgttttc acccggatat
    17881 ccagctgggg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccgttacggg
    17941 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt atgcgggctt
    18001 catactgggt atcgtttaaa cgttgtaacg tcagccagtt tgccggatgc gatagcagcg
    18061 actgctgttc aacgcgatcg cgttccagcg tcaataatgc ttcatcaatt ggctccggga
    18121 aggtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
    18181 ctaccgtacc cgtatgcgcc gtgctgccct taccgctgac tgcatggctt aacccggcga
    18241 gaattaagcc gtcttcatcg cgtaatgtca gattgtagtt gccaggttta tcgaaattga
    18301 cggtaaagga tttgccgcct gacggtagat ctccgctatg gctcgtgcgg tcttcgagac
    18361 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
    18421 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
    18481 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
    18541 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaaa gccacatgac
    18601 cgctatcatc ggacaccgtt tcgctgcctt ccagcgacac gggaaaacgt ccggcataac
    18661 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
    18721 cttttaccgg ctctccatcc gggtaaagca gttgcagttt gccgctcacc gcttcgcctg
    18781 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
    18841 ccacgcgaaa actgctgcta tagacctgtt tgcggtaagc caggcgtaac tcataacctc
    18901 cggcgacggc attttctggc aggcggaaac tcccctgccc gccattacgc gcatcaagcg
    18961 tgacattgac ggtttgcaac agactgccgt tggcgtccag cactgaaagc ttcgccgggg
    19021 cgctgacgat gggggatgaa tgcaacgggt cgtggaactc gcggcccatc actttgacat
    19081 caacacgatc gcctgcacga tacagagggc ggtcggtaaa gatatacaag cgggtgttgt
    19141 agatttcgct ttcgtagaaa aaattctcgg agacaaaaac gccgccttca gcatctttac
    19201 ccagaatgta tgaacgttcc ggcgatatat gttgtaactg caaggtaccg ctgtcatcgg
    19261 tcacaccgcg ggtcatcacg ccaagaccgt cagtccacaa gatctctgat cccggcttcg
    19321 cttccccctg ttttttaccc gcggtccaca ccagaagctc gttacctgac actttgctaa
    19381 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
    19441 cgacgaggta cagccccggc tcttgcttgc ctagcggaat atagatgttg cccggctgcg
    19501 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
    19561 tagcctgcca taatgggtaa cggaactgtt ccaccagggg atattttttc agcggtgaaa
    19621 actggttgtt ctgtacataa cgggaaggtt taataatggc attaccgagc tgtaattcgg
    19681 gtaacgcctg agtcacattc tgccgagact gagaagaaaa agtacgctgc atcacgcggc
    19741 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
    19801 aatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
    19861 ggtcaggaat acgatacagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgtc
    19921 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
    19981 tgtcagcgag caaaaagaat gttcccccgg cgggcggcgc atagttgctg gaaggaagcg
    20041 aatcatcagc attggcaagc cctgttccca caagagacag gaacaagcac gctgctatcg
    20101 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
    20161 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
    20221 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
    20281 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcctggcc
    20341 aataaattgg ctgttgtact gaatcaaatt aattgcggtc acgtaggggc cggttttccc
    20401 gttcccctga ttccagtttt gcgccagttg ccgctgttca ggtgttagcg tcatctctgg
    20461 cggcaaatac tggttagata acccgttact ttttagccat ttactgtcgt gaactttcag
    20521 cgtctcgttc gctgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
    20581 tggaccctgg cggagctgtt cctgtgcaat gcgtacaaac caggcgcgaa acagcccgga
    20641 ttgttcgaca ttcagcattt cgctatgggt aacaacacaa cacagccagc aaatcagcgc
    20701 cagcagcccg tgtctcatag cggttgccag gttatgggta gccactgcca ggcggcaccg
    20761 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
    20821 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcaaactg
    20881 gtcagcgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
    20941 atgccgtcag tgggtatcac atccaccatt gccgggcggg ttttattcag tgtttgcagc
    21001 gcgttattaa ccagcgtgtc atcgagggag aaaagcatcg tcttgttttg catcgccagc
    21061 gacacgcgga aaaaataatc cgacattaat tgatcgggtt gcaccgcctg cgctttcgga
    21121 tactggccgt atcgggaact cacttcgcga cgccagattt gcgcttcgcc ctgctgagtc
    21181 tggtttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
    21241 aacagtttcc caggcagttg tgcctgttcg gctgagccat caaattgacc aacaaatagc
    21301 ggggtttgta attttgagtc ttcataccag cacagcccgg ctgcaccatc taacgccccg
    21361 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accatgcgaa
    21421 tacggcaccg ccacacagaa gctggccccg gcaggcatac tgttccatac cggcgtaaaa
    21481 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaagct atgccagccg
    21541 tcgttgctca tttcgaagcg tacgccagca aaagaaggca ttaatcgctg gtagccaaac
    21601 cccagcaacc tggcgctgac aacaatacgc tggcgtactg gcgttttttc agcagcacgc
    21661 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
    21721 gccgtggctt cggtatcctg ctggtcatct ttaaacagca tatccgtact ggaaaacacc
    21781 agcatcttgt cctgataagt cgcgaacatc agggcgttat tgccgttata gcgcaactga
    21841 taaaccggga tggtttcact atttagctta atactactga tttccgtttt gcttaactgg
    21901 ctgtcgctgg tagcggcaaa caacaatggc tccaacagtt tgcttaaacc actgcgctgg
    21961 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
    22021 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
    22081 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
    22141 aaatcttcac tcagaacatc gtggagaaac ggaatggtaa ggaggtcttt gggaagctgg
    22201 ctcagagagt cgctgtcgag aaagaggtcc ggctcattga gatcgatttg cagattgttg
    22261 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
    22321 acgccagcgg aaagcagtac tattgcgccc aaacctacaa gaccatagaa ccgccagcct
    22381 ttcgcctttt tttcaccact cattgccaca ttccttgtgt aaagccagcc attttttacg
    22441 agcacagcaa aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
    22501 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
    22561 ttctggctcg tcgtcaacgt ccacttccgg agcgatttca tcgtcccctt ccgcggcact
    22621 gccatcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgtt gcagacccac
    22681 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcctacgat
    22741 gctgatttcc gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
    22801 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
    22861 ggagataacc cctttcgtcg cacgcgactt agtcgggtat tccgccactg cggtacgttt
    22921 accataaccg ttctgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
    22981 gacgacttta tcgccttcgc ctaaacgaat accgcgaaca ccggtggtgt tgcagcccat
    23041 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
    23101 ttcgtcttcg ccgctggtca ggtcaacgcc gatcagctca tcgccttcaa ccagtttgat
    23161 cgccacttta ccggcggtac gcagacggtt gaactcggtg aggacggttt tcttcacagt
    23221 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
    23281 cgcagtgata cgttcgtcct gctccagcgg cagcaggttg acgatcggac gaccacgcgc
    23341 gccacgagtg gcttctggta actggtagac cttcatcgaa tagacgcggc cacggctgga
    23401 gaagcacaga atatggtcgt gggtgttcgc caccagcagt cggtcgataa agtcttcttc
    23461 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcat attcagaaag
    23521 cggctgatac ttcacgtaac cctggtgaga aagcgtgacc accacatctt cctggatgat
    23581 cagatcttcc aggttgatgt cggcgctgtt agcggtgatt tcagtacgac gtttgtcacc
    23641 gaactgttca cgaaccagtt ccagctcttc gcggatcact tccatcagac gatcggcact
    23701 gccaagaata cgcagcagtt ctgcgatctg atccagcagc tctttgtatt cgtcgagcag
    23761 tttttcatgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
    23821 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
    23881 acgcgcagca tcatcgcccg cacgttcgag catggcggca acgttgccca gctgccacgg
    23941 attagccacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
    24001 gatcgggtcg atgttcgcca gcgctacggc taatgcttca aggatatgag cacgatcgcg
    24061 agctttacgc agttcgaaaa tagtacgacg agtcaccact tcgcggcggt gacgaacaaa
    24121 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
    24181 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
    24241 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
    24301 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
    24361 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
    24421 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
    24481 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
    24541 ggggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
    24601 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
    24661 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
    24721 ttccgtgccg tcatagttat ctacgaaatc gaccgtctct ttttcgagat cggccatcag
    24781 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
    24841 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
    24901 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
    24961 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
    25021 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
    25081 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttca gctcttcctc
    25141 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
    25201 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
    25261 cgctttattc acctccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
    25321 tgccaatgaa tgccgaaaaa tcgccggtaa accataacgt agaccacgaa gagatcgcta
    25381 aatttgaagc tgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgctgcacc
    25441 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
    25501 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
    25561 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ttgcacgcgc
    25621 tggaaagcgg cattcaggta gagtacgtac aggaaaccgt ggaagagcac gcggcaaaac
    25681 atgccgggca gtatgatgta gtgacctgca tggagatgct ggagcacgtc cccgatccgc
    25741 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
    25801 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggtgctgaa tatattttgc
    25861 gcatggtgcc caaaggcacg catgatgtga agaagtttat taaaccggca gaattgctgg
    25921 gctgggtgga tcagaccagt ttaaaagagc ggcatatgac cgggctgcat tacaacccga
    25981 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
    26041 ataagtgagg ttgttgtttg gattcatccg acaaccgtgc caatgcctga tgcgacgctt
    26101 gccgcgtctt atcaggccta caaatgctcc cgtaggccag ataaggcgtt tacgccgcat
    26161 ccggcaaaag tgccggtcag acagcgatta ccatttcacc gtcatcgaca aaaaccctgc
    26221 cgtctgggca aaatcatcac tccctttctg ccacgccacg ctgccgcgca gggacactcg
    26281 ctgactgata ttgcccgtga ctcccacttt tatttcaccc cgttgcttca ccccatcgtc
    26341 actgatagtg ctgccatcct cttcaatttc cgtcgcatgg ggatcgtgat aataattcag
    26401 atctaacgtt ggcgtgacac caacagcagt actccattcg ctgtgtaaac ccagccgcgt
    26461 ctgaatatca tcaccctgcg attgtgacac gcgcgcatgg ttagcggcgg taaaatcatc
    26521 ctgctgcacg ccctgataaa tcacctgcgt ctgcggttca atcaccacac catgccccgg
    26581 taaccactga taaccggctt ccagcgaggc gataatcccc gacgagtggt aatgatcctc
    26641 gccatcgtct tgttcggaaa catcattgtt aaaccacgca tattgcagcc agctatccag
    26701 ccagaccccc tgcttctgat taccgtgctg ataccagctt gaggtcagcc caacggcata
    26761 accgtggttc tggttatcgg cgcgagttcc ggtcatattc gagcggctgt cgccctggtt
    26821 atcgctgtag ccaccaacaa tcccaagcat ccactcgcca tcatcacccc agtgcccgct
    26881 aaacaggttg ccgctaagct gcaccgtaga agtgtcttca tgctgagcca actgccctgc
    26941 cgccgtgtaa tgataacgcc ctccgataac gcgtaaattc agcgtctgac catcgccacc
    27001 agcctgatcg cgtcgctcca tcacaaatgc ctgatttgcc gcacgtaggt tattaaaata
    27061 tccaccaact ttggcattca acaccggctg gtaagcaggc gtaggtccag ggtcgggggt
    27121 tgggtcggga tccggcgtgg gatcaggatc tggagtcggg tccggatcgg gcggcggcgt
    27181 cggagtgact tcttgcgatc gcagatacca gtcgttgtta tcttccacca gcgtatagtc
    27241 atacgccccc atattgacgt agccgcttcc tgccagacgg aactgcgcat tgttttgaaa
    27301 ctgcgtggga tcagctgcga aatcaaccac tttaatacct gtagatgtcg gctcaccaat
    27361 acccgtaatg gggttaatta ccacggccgt gttgccagca gtattaccgt tcaataccag
    27421 ttggtcactt gccgagtcat cgccgtttaa ttcgctgtcg agcagtagcg taccgccgcc
    27481 ggtataatca ccatttaccg tcagcgtgtc gccagcgacg ccattttgca ggctaatcgc
    27541 accggacgta ttggtcagat tgccattaac cgtcacgtcg gaaagataag tcaggctgtc
    27601 agtttcaagg ttggtattaa gctcgcctgt cagcgtcacg ccatcggtgg cattcaccaa
    27661 cgtaccgtcg ccgctaaaca gcgaggcatc cagcacctgg ttcgtatcct gctgcgtcaa
    27721 tcgcaacaca gtaccatcgg ttatgtcgat atcgccgcca gaggtggcat cgattgagcg
    27781 aatatcctga ttttgcccgg tttcaaaaac accgtcttcc cccacccaca gtgaagaaac
    27841 ataaggaata atattctccg cttcaccttt tagcgttccg tcctccacac gtaccgcaga
    27901 cgtcgcagtg ccgctggcgg tcaactccag cgtaccgccg ccacttttag tcaacgtgct
    27961 tacgctctcc agttgttgcc cggtactatc cgccatcaac ccgccccatt gcgtatctac
    28021 gccatcctca acgtgtattt cgccatcagc gcgcatttca atattcctgc cctgacctgt
    28081 tgtagccgag cgagtgtcaa catcagtcgt cacatccatt tcactgtgtt gttggggatc
    28141 tgtgaagatc actgaacggt tgtaacttgt gtcccccaat tgcgaattat cgctgacttc
    28201 aagcgtaccg gaggcgattt gcgtattacc gagataaccg ttgttattcg ccagcgttac
    28261 gcgctgatcc cccgcattaa tgacgacata aacgccattc tggctggcat cactgccgcc
    28321 aatatgtaaa tcatcgccac tgctgatatc aacgacatca ttactgccaa tcactgtccc
    28381 cccgctgccg ctgatttgca gaccgtcatt gtatgagcta tcgccatccc aggtggtgaa
    28441 atcagagaga tccaatactc cgccatttag cacgatcgat tgcggatcgc tctgcattgc
    28501 ccgtaaatcg gcctgattcc ccgctagcga caatacggcg ttatcttcca ccacaatatc
    28561 gccggttaat gccatcgact gcgcccctgt caggagatag ctgccatctt gcgctaccgt
    28621 caactgtcct tcaccctgaa tcgagccgga aaaaccgccc tggtttacgg taacattgcc
    28681 gcctgcgtcg atattcaaaa tgccattagc aaaaccggtt aatgagtgca caaacgtttg
    28741 ttgtgtattg ccaacattca gttctgcctg atactcagaa tgaacagtgc tgccgaccat
    28801 cagaccaaag caatcctgcg gatcgctctg acagtgcgtg tcgccgacat tcattaactc
    28861 atcgctgcgc cccaacgtca cctcgccatt ctggatactt atctcgccgg taaacgcatt
    28921 attgtcggca ttgaggacta actctcccgc tccctcttta acgatgactc ccgttccggc
    28981 aagagaatca atcgcgccat cgttgctggt attgcctatt accagcgttt tatcggcggc
    29041 aatgttaaat tcagccacac tatgaccgat atacataaag ccccccgccg ctgccgtcgc
    29101 cgaatggtca tatcccgagg cgctgttatt ttcatcaatc atcacaccat tgttttggtc
    29161 ataattatca tcaacagaaa tatcaataag atacggggat gtggtgctgc tggtataaat
    29221 tgctccgcca taaccttctg catagttatt ggtaaaggag gtattattaa ttatcgtttt
    29281 tcctgaaaga tgtgtgttat ccgtactatt atccgtaaca tcaatagcgc cgccatcccc
    29341 atcactataa cttgttgatg tatatgcctg gttattatta aaagcatcat cacttaaata
    29401 gacgtcatta ttgatggtat aaattgcacc gcctttgccg tcattagcga tgttattgtg
    29461 aaatacggcg ttagtgacac gtaaatcggc ggcaccggta tcattggtac cggcagaata
    29521 gattgctccg ccatagcctc ctgcaacatt actgtcgaaa ataacattcg caagatttat
    29581 cgttgagttt tctttagcaa agatagcccc accattatta tattcgccac taaccgagtt
    29641 atcagcaaac agggtcatcc ctgtttcatt ttgtggtgaa atcgtaaatt cagcactttg
    29701 ctgcaaaaat actgcgccac cgctggcatt attggccata cccgaaaaaa tcaaccattg
    29761 cccatccgcg atgctccagt cctgcatatt gcccgaaagg ctttgcctgc tggcctgaca
    29821 gttcgctttg atatcatatc cctggcatga atcgatgacc gctgcggcac cgttaccaga
    29881 gaaaagagat gcaatcattg acgggagtaa agataaatac tccttgcgta gaaagataat
    29941 ccgcatattc atctaaccat cattttctat aagatggcgt atttaatcgc attatgcgat
    30001 atagggtatt tttctttt
//
LOCUS       DAZGHF010000051        29797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGHF010000051 DAZGHF010000000
VERSION     DAZGHF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.051"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..818)
                     /locus_tag="ACHK3A_002365"
     CDS             complement(<1..818)
                     /locus_tag="ACHK3A_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO8740431.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDVEYIFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQIITELIEVVYNRKVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKDIGDIYEQFLEKDILLQRAIHEIERYLYATFKYELILEEKLYLSIRTKVL"
     gene            complement(1076..2329)
                     /gene="amiC"
                     /locus_tag="ACHK3A_002366"
     CDS             complement(1076..2329)
                     /gene="amiC"
                     /locus_tag="ACHK3A_002366"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO8740432.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2561..3892
                     /gene="argA"
                     /locus_tag="ACHK3A_002367"
     CDS             2561..3892
                     /gene="argA"
                     /locus_tag="ACHK3A_002367"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO8740433.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(4130..5956)
                     /gene="recD"
                     /locus_tag="ACHK3A_002368"
     CDS             complement(4130..5956)
                     /gene="recD"
                     /locus_tag="ACHK3A_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO8740434.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPSGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5956..9498)
                     /gene="recB"
                     /locus_tag="ACHK3A_002369"
     CDS             complement(5956..9498)
                     /gene="recB"
                     /locus_tag="ACHK3A_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO8740435.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKLLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALMLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVDEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDSLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGITLEEA"
     gene            complement(9491..12379)
                     /gene="ptrA"
                     /locus_tag="ACHK3A_002370"
     CDS             complement(9491..12379)
                     /gene="ptrA"
                     /locus_tag="ACHK3A_002370"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO8740436.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12555..15923)
                     /gene="recC"
                     /locus_tag="ACHK3A_002371"
     CDS             complement(12555..15923)
                     /gene="recC"
                     /locus_tag="ACHK3A_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO8740437.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWEIGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDADRLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIARRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15936..16259)
                     /gene="ppdC"
                     /locus_tag="ACHK3A_002372"
     CDS             complement(15936..16259)
                     /gene="ppdC"
                     /locus_tag="ACHK3A_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO8740438.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(16244..16651)
                     /gene="ygdB"
                     /locus_tag="ACHK3A_002373"
     CDS             complement(16244..16651)
                     /gene="ygdB"
                     /locus_tag="ACHK3A_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO8740439.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16648..17211)
                     /gene="ppdB"
                     /locus_tag="ACHK3A_002374"
     CDS             complement(16648..17211)
                     /gene="ppdB"
                     /locus_tag="ACHK3A_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO8740440.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(17202..17672)
                     /gene="ppdA"
                     /locus_tag="ACHK3A_002375"
     CDS             complement(17202..17672)
                     /gene="ppdA"
                     /locus_tag="ACHK3A_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO8740441.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17857..18651)
                     /gene="thyA"
                     /locus_tag="ACHK3A_002376"
     CDS             complement(17857..18651)
                     /gene="thyA"
                     /locus_tag="ACHK3A_002376"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO8740442.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18658..19533)
                     /gene="lgt"
                     /locus_tag="ACHK3A_002377"
     CDS             complement(18658..19533)
                     /gene="lgt"
                     /locus_tag="ACHK3A_002377"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO8740443.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19684..21930)
                     /gene="ptsP"
                     /locus_tag="ACHK3A_002378"
     CDS             complement(19684..21930)
                     /gene="ptsP"
                     /locus_tag="ACHK3A_002378"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO8740444.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21943..22473)
                     /gene="rppH"
                     /locus_tag="ACHK3A_002379"
     CDS             complement(21943..22473)
                     /gene="rppH"
                     /locus_tag="ACHK3A_002379"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO8740445.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22826..22972)
                     /gene="ygdT"
                     /locus_tag="ACHK3A_002380"
     CDS             complement(22826..22972)
                     /gene="ygdT"
                     /locus_tag="ACHK3A_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO8740446.1"
                     /translation="MLSTESWDNCEKPSLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            23158..23847
                     /gene="mutH"
                     /locus_tag="ACHK3A_002381"
     CDS             23158..23847
                     /gene="mutH"
                     /locus_tag="ACHK3A_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO8740447.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23916..24629
                     /gene="ygdQ"
                     /locus_tag="ACHK3A_002382"
     CDS             23916..24629
                     /gene="ygdQ"
                     /locus_tag="ACHK3A_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO8740448.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24767..24985
                     /gene="ygdR"
                     /locus_tag="ACHK3A_002383"
     CDS             24767..24985
                     /gene="ygdR"
                     /locus_tag="ACHK3A_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO8740449.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            25093..26133
                     /gene="tas"
                     /locus_tag="ACHK3A_002384"
     CDS             25093..26133
                     /gene="tas"
                     /locus_tag="ACHK3A_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO8740450.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(26165..27358)
                     /gene="lplT"
                     /locus_tag="ACHK3A_002385"
     CDS             complement(26165..27358)
                     /gene="lplT"
                     /locus_tag="ACHK3A_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO8740451.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLSAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRY"
     gene            complement(27351..29510)
                     /gene="aas"
                     /locus_tag="ACHK3A_002386"
     CDS             complement(27351..29510)
                     /gene="aas"
                     /locus_tag="ACHK3A_002386"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO8740452.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
BASE COUNT         7329 a         8076 c         7363 g         7029 t
ORIGIN      
        1 attaatactt tggtacgaat agagagatat agtttttctt ctaaaattaa ttcatattta
       61 aaagtggcat acagataccg ttcaatttca tgaattgcgc gctgtaacag gatgtccttt
      121 tcaagaaact gttcatagat atcaccaata tctttctgtt ttattttttc attgcataac
      181 acgcgacttg aaaaatattt aaggtgcatc ataaatctgg aatagttaaa tgaacttctg
      241 tccacctttc tgttataaac aacttcaata agctccgtga ttatttgcga caactcaata
      301 atccgtactg attcataatc aactgaattt tcataattat ttaaaatatg caatgctata
      361 aaaccaatct catcatcagg aagttgaacg ctgaattgct cattaatgat agttaatgca
      421 tcgcgactta tcgcatactc tgctttgtac aacgctttga tttcatcaag gaaaacatta
      481 ttaatttgta tacctgaaac tactcgggat attgcattcg aaatgtgatc cgcaagaatc
      541 aatagtagcg atggattgag atttttttcg agatttttct ccgcatattg cactattttt
      601 tcggcaacaa aaatatactc aacatccaca tgttcaatca atttgtagag tttatttttt
      661 tgttcgtttc tgacataaaa aatccggtta gccggatgtt cagggaccga cattccatat
      721 tttttgttgt aaccgacgcc aggtccagaa atgataacct cctgtccatt catcgatgcc
      781 tgtacacaat tattgttcat gactttttcg atgatcattc catgactcca aaaaaaaagg
      841 caaggcacgt cagaagtaat aattacttca aacattgcct tgcctgattt aacagttaca
      901 agatgattaa ctaatgcgta tacattacac ggaattgtgc ttaattaaag agatggtcgg
      961 cttatttttc aagccgacat tcaaatattc gacatcagtc acaaaaccag catctaatat
     1021 tcatgaaaat gttatctatt caacgaaata taaattcatt gaataaataa aaacatcatc
     1081 cccttctcgc cagcgtcgcc ccatcggcaa aatacgcttt aatccccgca agaatagact
     1141 ccgcaacttc ctgctggaaa gtcgccgttt tcagtttacg ttcttcctca acgttactga
     1201 taaacgccgt ttcgaccaga atggagggaa tatctggtgc ctttagtacg gcaaacccgg
     1261 cctgttcaac ttgattttta tgcagcttgt tgattttacc gagcttattc agcaccgctt
     1321 taccaaactt caggctgtcg gcaatggtca gcgactgcac catatcgaac atggtgtggt
     1381 cgacatagcg gtcaccgctt ttgctcacgc caccaatcaa gtccgaggcg ttctgggttt
     1441 gtgccagata ttttgccgca gtactggttg cacctttggt tgagagcgca aacacagagg
     1501 aaccgctcgg ctgacgactg gtaaaggcgt cggcatggat agagacaaac aggtcagcac
     1561 gctgtttctg ggcttttgct acgcgcactt gcaacggaat gaagatgtct tcattgcgcg
     1621 tcatgtacac cttcatattg ccctctttct cgatcagaga gcgcagacgg cgagctattt
     1681 gcaataccac gtctttttcg cgcgttttgt atttccccac cgcaccggag tcttcgccac
     1741 cgtggccagg gtcaagcata atgacaatag gacgatcgcg ccctgcttta cccggttgtg
     1801 gaccactttg tgctggcggc acctgctttt cgaggtcgcc tttgttgtaa tcctccagca
     1861 gcgccagcag cgggtcctgc atatcctgtg cattggccgg atagaggtcc atcaccagac
     1921 gctctttaaa cccggcgacc ggcgcaaggg caaacagctg cggttttacg ttttgcttta
     1981 attcaaaaac catacgtacg gtttgcgggt caaattgccc gacgcgcgcc gacttgatga
     2041 acgggtcgtc agcgcggatt tgcgcagcca tccccttgag taccgagttc aggtttacat
     2101 cttcgatatc caccaccacg cgttcaggat tactcaacgc gaactgctta tatttcagct
     2161 gacgatttga ttctaccgtc acgcgggtgt agctggacgc aggccagacg cgcaccgcca
     2221 cgacctggct gaccgcagcc aggctgacct gacttacgct caatagccac atggcacccg
     2281 cgccttgcag taaacgacga cggctgattg cagtgttgga tcctgacatg cctctcccga
     2341 gcaaaagaaa tctaagctgt gtaacaagta aacgaccaat ttgaccggaa actttagcga
     2401 aagacgcata atctgtcatc tataaaacgg taaacattct ttttatattc acggtattac
     2461 tgataaaaaa ctcgctcccg cataaaattt acacttgcac cctgcgaaaa aacagaataa
     2521 aaatacaata atttcgaata atcatgcaaa gaggtgtacc gtggtaaagg aacgtaaaac
     2581 cgagctggtc gagggattcc gccattcggt tccctatatc aatacccacc ggggaaaaac
     2641 atttgtcatc atgctcggcg gtgaagccat tgagcatgag aatttctcca gtatcgttaa
     2701 tgatatcggg ttgttgcaca gcctcggcat ccgtctggtg gtggtctatg gcgcacgtcc
     2761 gcagatcgac gcaaatctgg ctgcgcatca ccacgaaccg ctgtatcaca agaatatacg
     2821 tgtgaccgac gccaaaacac tggaactggt gaagcaggct gcgggaacat tgcaactgga
     2881 tattactgct cgcctgtcga tgagtctcaa taacacgccg ctacagggcg cgcatatcaa
     2941 cgtcgtcagt ggcaatttta ttatcgccca gccgctgggc gtcgatgacg gcgtggatta
     3001 ctgccatagc gggcgtatcc ggcggattga tgaagacgcg atccatcgtc aactggacag
     3061 cggtgcaata gtgctaatgg ggccggtcgc tgtttcagtc actggcgaga gctttaatct
     3121 gacctcggaa gagattgcca ctcaactggc catcaaactg aaagctgaaa agatgattgg
     3181 tttttgctct tcccagggcg tcactaatga cgacggtgat attgtctccg aacttttccc
     3241 taacgaagcg caagcacggg tagaagccca ggaagagaaa ggtgattaca actccggtac
     3301 ggtgcgcttt ttgcgtggcg cagtgaaagc ctgccgcagc ggcgtgcgtc gctgtcattt
     3361 aatcagttat caggaagatg gcgcgctatt gcaagagttg ttctcacgcg acggtatcgg
     3421 tacgcagatt gtgatggaaa gcgccgagca gattcgtcgc gcaacaatca acgatattgg
     3481 cggtattctg gagttgattc gcccgctgga gcagcaaggt attctggtac gccgttctcg
     3541 cgagcagcta gagatggaaa tcgacaaatt caccattatt cagcgcgata acacgactat
     3601 tgcctgcgcc gcgctctatc cgttcccgga agagaagatt ggggaaatgg cctgtgtggc
     3661 agttcacccg gattaccgca gctcatcaag gggcgaggtt ctgctggaac gcattgccgc
     3721 tcaggcgaag cagagcggct taagcaaatt gtttgtgctg accacgcgca gtattcactg
     3781 gttccaggaa cgtggattta ccccggtgga tattgattta ctccccgaga gcaaaaagca
     3841 gttgtacaac taccagcgta aatccaaagt gttgatggcg gatttggggt aagtggtgga
     3901 aatcgccaga tgcgacatgc gcaatgcctg gaggcatgat aaacacgcta gcatcgcatc
     3961 aggtattgac gctcatcgtc ggatgcggcg tgaacgcctt atccgaccta catcaaatca
     4021 aaggtgttga tggcggattt agggtaagcg acggaaatcg ccagatgcgc atatgcgcaa
     4081 tgcccggagg catgacagga cgcgcatgcg tcgcatcagg caatcacgtt tattcccgcg
     4141 aactaaacaa tgccgccaga ccactgcgcc gctcagtgcg cgtggcaatt gccgcactta
     4201 aaatgcgctc atcggcatac agcgacagac ggcgacgcgc gcgggtcact gcggtgtaaa
     4261 ccagctctcg cgttactacc ggcgtgcgtt gactcggtaa aatcaacgcc gcatggtcga
     4321 actccgatcc ctgcgattta tgtaccgtca tcgcccacgt cgtttcgtgc tctggcaggc
     4381 gactcggttg cacagactta atattgccgt ccggcatcgc aaaccaaacg cgcgtcccct
     4441 gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac
     4501 gggcaatcat caccggtcgg ccttcgtacc aacgagagtg cggattacga tgaattttgc
     4561 gcttctgttg cataaactgc tcaattcgct cattcagtcc agccacgcca aacggccctt
     4621 cccgcagggc gcacaaaagc tggtactcat tgaacgcctg aatgattaaa tccggctcgg
     4681 cacgcgcttg cagcagatcc agataacgcc cgtaacccgc aagagcctcc tcaagcatcg
     4741 caatatagtc ttcgccgctc tgtaagagcc gtttttcgat atcagtaaaa tcctgctgaa
     4801 aaacggtttt cactgccgtt ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc
     4861 cagaatcgct gccgaaacga tagctttttt gcagcaggca gagactgtcg cgcaaagatg
     4921 ccgcttctgt gccagttcct gacggaacgt gagttcccgt caggcggctc aactgcccgg
     4981 cacgctcggc ggtaaagccc gcgttggcat aagcgcagat atcgcccagc acagctccag
     5041 cctcaactga ggccagttga tcgcgatcac cgaggaagat cactcgcgca tgatcgggca
     5101 acgcatcgat caggcgcgac atcatgggga gatcgatcat tgacgcttca tccaccacca
     5161 gcacatcaag atgcagcggg ttaccggcat gatgacgtaa acgctggcta cccggctgcg
     5221 cacccagcaa tcggtgcaaa gtgctggcat cttccggaat gcgtttcttt tgttcatcgg
     5281 tcagcggtaa ctggcgcaaa gccttgccga gagattcggt taagcgcgcg gcagctttac
     5341 ccgttggtgc agccagacgg atacggcagc gttcgccgtc tgccatttga attaacgctg
     5401 ccagcaactt cgctacggtg gtcgttttac cggtgccagg gccgccggaa atcaccgaga
     5461 tccgccgcgt cagcgccact gccgccgcca ctttttgcca gttaatttca tcgcttactg
     5521 gaaaaagttt gtccagggtt tgcgccagta gagcttcatc aacctcaatg gcatgattca
     5581 cttcgttgaa aaagcgtgcc actgtgcgct cgttacacca catgcgattc aaataaagac
     5641 gatcaccaca gaggatcatc ggcgttggtt catctccccg gctgaccgct tgtgaagcca
     5701 gcaagcattc ttcccaattt tgtaactcac cgatttcgct gacgcaggtt gctaacagcg
     5761 gatgtgacgc ctcgttattt tccagtcgtg agagcggtaa acagacatgc ccttccccgg
     5821 cgtcgtggct taacagggct gccgcgaggg tgacggcagg atgttcatct cccgccacgg
     5881 tcagggcaaa ttgcacatcc agcgggcgta actgtttgtg ctccacagct tccagtaatt
     5941 gcttttgcaa tttcattacg cctcctccag ggtgataccg gcaaacatct catccatcag
     6001 gtcaatcaac ccggcgttgg gtcgggtcgc gtagatccct tgttgcggat gttctttatc
     6061 aacaccacgc aggaacagat aaataacgcc gccaaagtgg cgctcatagt cgtaatcagc
     6121 aatgcgatgg cgcagataac ggtgcagcgc cagggtataa agctgatatt gcagatcata
     6181 gcggtgtgcc tgcattgccg ctgccatagc ctgttgggtg taagcagaac tgtcttcacc
     6241 caaccagttg gatttatagt cgagcaggta ataacgccct tcgtggcgga acaccaggtc
     6301 aataaagcct tttaacatgc cgcgtacctg catgaactcc agcggcgggc agccagcgga
     6361 tagcgagtca aactggcgaa ttagtgcatc aagctggctg gcgatgagcg gttcgctaat
     6421 cggcaggtaa aactccatct ccacctgttt atcgcggtcg gaaagctgac tcaggctaac
     6481 acccgtttca ttgagaggtg cctggaggac agccgtgatc cactcggtca acaccggttc
     6541 ccactgcgat tcaaagccgc cgagttccag tttttcctgt acccagttcg ggtcaaccgg
     6601 ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat gtccccggtg acgcaccgcg
     6661 cgggaactga tgcggtgtta acgtcggttc gtcaacgacg ctgaccacgc ctgcggcatc
     6721 aacatccagc cgtggcatca gatcctgagc gataccgtga ccacgctgct gcaaaccgga
     6781 gtagctggtg acacgccagt tatcgccggg caatcgttgt agcgtcctcg cattcagttc
     6841 tgcagttata gcatcattaa cctgccaggg ctggttatca ccagtttgtg ccgtttgcca
     6901 ggcaatatca tcatcgcata acgcttcaat acaggtgcga agccctgccg catcttgcgg
     6961 ttcccctttt tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt cacctttttt
     7021 atcgccacga cggcgcacca gcggtgcaac gccgagactg caatgccaaa ccgaacgcgt
     7081 cagcgccacg taaagcaaac gcagatcttc cgccagacgt tcggcctccg cgagatcgac
     7141 gctttctggc gcagcattaa gatccagaac agcctcaaac gagtggcgat cgtgatagaa
     7201 cgcctgatcc tggacgcgga aattggtaat aaatggcaac cagaccagcg gatattccag
     7261 cccttttgat ttatggatcg tgacaatctg caccagatgt ttatcacttt cgaggcgcat
     7321 ttgttggctg gaggcattac tgtctggctc gaggatatgt tgcgataacc agcgtaccag
     7381 cgcatgttca ctttccagct gcgttccggc ttcttgtagc aattcgctga tatgcaagat
     7441 atcggtaaga cgccgctcac cgcctgctgt tgccagcaag ttttctgcaa tgttacgcgc
     7501 cgacatcagc gcccgcagca tcggcataac gccacgtttg cgccagattt gccgataacc
     7561 atcgaactct tcgacgaccg catcccacgc atgttcgtca ttattcagcg tttcaatatc
     7621 cagcgcattc agccccatca ttgacgttgc cagcgcactg cgcagggtgt tctcacgttc
     7681 gggagtcatc accgcctgca acagccaaag catctcctgc gcttccagag tttcaaaaac
     7741 actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc agcattaagg catcgcgcac
     7801 ctgggcggcc tcctggcggc tgcgcaccag cacactgatg tccgaagcac gcaccggacg
     7861 cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt ccggcttgta gccagtcgcg
     7921 gatttgcgca gcacatacct gcgccatggt actttgatag tcgccaacgc cgcagctttc
     7981 gccttccatc agccacattt tcatcgcagg ctgcgtttca cctttaaaca caaaacgtaa
     8041 cgcctgattt ttgccggcag atttcacagg aataaacggt atttcacgaa acatgaacgc
     8101 gtcatcagtc tggctgaaaa gtttgttaac gctgttcacc attcccggtg cggaacgcca
     8161 gttggtatct aaggtgtagt gagcatgaac ttcgctacgc gccttcatat aagtgaagat
     8221 atccgcaccc cggaatgcat aaatagcctg tttcgggtcg ccaatcagca acaatgcggt
     8281 ttccggctga tggtgccaga tacggcgaaa aattcgatac tgctgggggt cggtatcctg
     8341 aaattcatcg atcattgcca ccgggaatcg cgtacggatc gccgctgcca atacctcgcc
     8401 gctttcacta cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac ccaattcgcc
     8461 acggcggcgt ttttcacgcg ctactgtttc gcggatctca gccaatgcgc gggtgatcac
     8521 cagatcgcgg atcgacaatg gttctgcaag cagttgatcg atcgcctcaa acagtggatg
     8581 tcgcggggtt tcccccccgg ctttcgtgcg atcttctaag aaacgctgag agaatttttc
     8641 cagcgactcc ggcaactgat agctgttggt ctcttcttct gcccaggcgc tgatcttgtc
     8701 gatccattta gcctgattgc tacggttaaa cttgcgtcga tcaataccag aagattcgat
     8761 cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt tttacggcat caatacgcgc
     8821 cacaatttgc gcgtggcggg aagcaagcgt ttcatcatcg ggcggcggtg ctttgataac
     8881 cggtgcttcg ccttgcagat aacgattaat atcgcgcagc aacgcctgcg gccctttcca
     8941 ggtttcaaag accacctggg caatttcacg cggcagcggg tagcagtggc gacgccagaa
     9001 atcggcgcag gcctggtagc gtagcagaga ctcatcttca atcagctgct gctcaaacag
     9061 catgccggat tcaaaggcat tcaggttgag catgcgctgg caaaaaccgt gaatagtaaa
     9121 gactgccgct tcatccatct gccgttcggc taacaacaac cactgcgcgg cttgcgcttt
     9181 atcgtcgatc tcctccagca ggagtttgta cagtggattg tcggtggttt cacgcagaca
     9241 ggcgatgcgc aactcgtgga tattgctacg gatacgaccg cgcaattctg ccgtggcagc
     9301 ctcggtaaag gtgaccacca gcagttcttc aacggtcagc gggcggggaa aggcggcaga
     9361 accgcctagt ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt
     9421 gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag gatctagtgt
     9481 ctcggcgaca tcactcattc ttttcactca tcaggggcat tgtttgctgc aacgcgctga
     9541 cgttctccca cactttccag ccttcaggat gcacatattc ggctttcccg ttctggctgc
     9601 cggaaatctg cgacagaata gccatgcctt gcggctcgac caccgcctga tggaagaaat
     9661 cagcaagttt ttgcggcgtc agcagtttta tctgggccac gattttatca cgcgaatcga
     9721 agcgcatatt gccgcgatcg aaatctttac ttaacttcga tgcttcttcg ccgagcgttt
     9781 gcggtgcctg cagcatctgg gttattaccg cctgctggat ttgcgcaaac tcctctggct
     9841 tcatcgcccg caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga
     9901 atgaaggctg tttatcattg ctttgcaaaa ggaagcccat gccccactga cgccccacgc
     9961 tcattggaaa cgcaaacacg gcatagccca actgttcttc ggtacgcaac tgattgtaga
    10021 accacggctg tacgatctgc cccaacagag agctataggc tgagctggtg tattcatcgt
    10081 agccagtcgg tacaaatacc gctgccagtg cggagtcggt gctgttaccg gctttttcaa
    10141 agatgacaga ttgtttttta tcgaccacga catctttgtt acggcaccac tccgaaccat
    10201 cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt tgcctgggct tcggtcatgt
    10261 tgccaataac cataaactct ggtcgagccc ctgattttaa ggcgtcgcga taggccagca
    10321 cctctttcaa agtgattgaa ggcaaaattt tgcgccgttc atctcgcgag aagtacggca
    10381 cttgcgagag catctgcgcg ggcataatcg cctgctcgaa cgctttgccc ttttctgcgg
    10441 aatccatcat ctggttatac caggacttcg cctgctcaag ctgatcttcc gtagcggtat
    10501 agctaaagta cccctcgagc aatgcctgga acagctgcgg cagacgctgg gtgtaaccat
    10561 tagcattaac cataaggccg ttgttagcgt tggtggaaaa actaatgcca ccaaccgacg
    10621 cctggttgct taactgatca agcgctagcc cggcaagata atcattgagc gcaaacatca
    10681 cctgattgcg ggcgctgtcc atggctttcg gattacgcaa aatcaggctg acatcagctt
    10741 tgggttcgct ggaaaaataa cggcttggcg catagaccac acgcagattc gactcatcaa
    10801 caataagctc tggatggtca tatttcttct ctgacttaat cagcgagaaa tcgtcaggaa
    10861 tataggggtt aagctctggc aaagagagcg caatgtcggc ggcttttttc tgccagtcag
    10921 cgaaagtttg tgcgctaatt ttatcgacct gatacggcgc atcgacaaag taagccgttt
    10981 tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc attctgcggc gtcatcatcg
    11041 ccaggcgttc ttttactgct ttagcgtcgt accgatcggc aatattgact gcatccagcg
    11101 tatgctcaac aggaacgcga atcatggtat ctgccagcca ttcgacgtaa tccatatcac
    11161 gggtgattga cggataacga aagtcgatat ccagcacatt cgccagttca tcgaagtatt
    11221 gtttatcaat ccctttttcg cgtaacagat tgaggtagct aaaaatggcc gccacaacct
    11281 gatcgcgatt cgccagacct ttatcggtta aagacgcaga gatcgctaat acgccgctgt
    11341 tgccgttgac gataggatcg gaattggcgc taatgccctc aactaatccc tgcttttgca
    11401 gccagtcaga aagtgtacct gggctgcgat taccgatcag ataggtaatc aattcatccg
    11461 ttttactacg gaacttcgct gaattgttat cgatgcgaaa ctcaacgcgc agaactttac
    11521 gcggcagcgc cgggacgtaa tgaatgataa tacctttttg cgcgtcggtg actaccggca
    11581 cggtgatttc cggttttttg ctctctttgt tcggcacgcg accaaaggtg tccgccgcca
    11641 tttttgccaa ctccggcagc ggtttattgc tgtaaataac cgccttcatc agattggcag
    11701 agtaatattt ctcgtgaaaa tctttcagcg cctgctgcac cggatttccg ggtttgtcgc
    11761 ttaaagtttc gaggttacca ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt
    11821 ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt aattcggcat
    11881 tcactgcatt acgttcacgt tcggcgtatt tcttgtcgag caagggttct gcaatagcgt
    11941 ccgccaggcg gtctaccgca ccgggcaagg cgtcgttctc aacttccaga tagaaagccg
    12001 tgcgatacgg tgccgtgctg gcattgtgac tgccgccgtg cattttgaga tattcggcca
    12061 gactgtcagc ctgcgggtac tttttcgacc ccatcagact catatgttca aggtaatgtg
    12121 ccagcccctg gtacgcctcg ggatcttcca gcgacccaac gggcactacc agcgccgaga
    12181 gcgatttaac cgcctgcgga tcagaaacca gcaagaccac cataccgtta tccagacgta
    12241 tagcctgata ctggcggtta tctttatcac ttttacggat ggtttcctga atcggctgcc
    12301 atcccgtttc tgcctgactt aagggtgccc aaagggcaac aaacaacaat aatgctttga
    12361 accaggtgct gcggggcatt cacggacctc ataatcaact ttattttctg gccagattca
    12421 acaggttaac gcaaatcatc tgccagaatt taatcttgtg ctgaacgagt cagcctatgt
    12481 ctatatatcc atcagtccgt gactggtgcg catcataaag caagcagata tattgcgcaa
    12541 tttttataca gcactcatga ctgattaaag cgaaacagcg gtaacaggaa acgttgcgac
    12601 tgttcaacga tagcctccat tgtctctggt gttaattgcc gccagagccg ttgataccag
    12661 atatcatcac cttcgccacg caccatcatg ttgccttcgt aagcctgaag gaatttcgta
    12721 cgggcttttt gtaacgtgga atcgtcatcc agcatggcat cgttttgcgc gtcataacag
    12781 gtttttagcc acgcgccgcc actttcaggt aacaccagca atggcgcgga cattccttca
    12841 cgatacccct caatcagttg tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga
    12901 aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt caccattacc accgctggca
    12961 cagtagacaa ggtgttccag ccaaagttgc attccctgcg ccacacttaa taaagaggga
    13021 cgccagcgca acaggccatc cggctgcacc tgcggcaacc agccagttat ctgcacaccg
    13081 ttgcaggcga gatcaatttc catactctgc cccggctggc gacgtgcaat gactctgtcg
    13141 gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa tttcaccaaa agcgccatac
    13201 ggtaaatccc ctgccgcccg gaagcggcgg aataagcgat cggcatcatc ctgctcaacc
    13261 agtgcattca ataactgctg attgatttga taacggctaa gcccttccag aataaatggc
    13321 tcggtgtcgg ggatttcgct gtcttcagta cggaagttca cctgcaaacg catctggaag
    13381 aacgcccgta ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt
    13441 aaggtaaacg gcagcggctg aacaaattca gaatgtgctt taccagcctg gctggccgca
    13501 ggtagccatt cacgagcata gctttgtcgt tcgcctggct ggtagttttg cggatcaaac
    13561 ggcatccggg tatggaggca agtaagatgc gcttttaccc ttgcctcgct ttcatcacag
    13621 ttgagcgctt catcgcccgg tagataatgg ctttgcccga tgtagtcgat cagttcctgc
    13681 accagtaccg acgggaaacg ttcactgtta tcctgaatag aacgcccgat atagctgata
    13741 tagagttttt gctgcgcgga aattaacgct tccaggaaca gatagcggtc gtcatcgcga
    13801 cggctacggt cgccacgctt cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc
    13861 tggcgtggat aaacgccgtc gttcattccc agcaggcaaa ccactttgaa tgggatggaa
    13921 cgcattggca tcagagtaca aatgttaacc ggtccggcga gaaaacgctg gctgatacgt
    13981 tcctgatcca ggcgctgtgc cagttcatca cgcaatagtg acagcggcac cgcgtcgcca
    14041 tactgcgcac ctaaaccttc ggcgataatc gcctgccatt gttgttcgat cagcgtcata
    14101 gccgcttcgg tttccgcatc cggcaggaag aaggcgttga gcatatcgcg acaaaccggc
    14161 aaccactctt ccagcggacg ctcctgcgcc agcccgcgac gccagatgtt tagctgcatt
    14221 agcagtgaag ccagatgccc caccagttct gcaattaagc cgctcgattc atcataaggt
    14281 agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa catacgcgtc
    14341 aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg
    14401 tcatctatcc cccaacgaat gccggattcg ttgacccact ggcgtaaata acgcagccct
    14461 tcttcggtga tgtcaaaccg cgccgccagc accggcacat ccagcagcgc cagcacatcc
    14521 tctgacacaa agcgactgtc aggcagtgat aacaggctga taaacgcttc caataccgga
    14581 tgtgactgcc gcgcccgacg gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg
    14641 ctaccaaaca cagcctgaat aaacggactg tagctgtcga tgtcggccac catcacgatg
    14701 atgtcgcgcg gagtcagtgt cgggtcttcc tccagcatcg ccagcaggcg atcgtgtaaa
    14761 acttcaactt cacgctgcgg gctatggcaa acgtgaaagg tgatactgct atccagtgga
    14821 tcaagcgggc gtttgttatc gctacgggaa aactcttcga tgtttacacc agcaacggcg
    14881 cggttttcca gttccagaat gtcagactga atgttatgca gcaggttatc tggcgtaaca
    14941 tcgacaaaag cgtccagctc ctggctgctc tccagatcag aaaggagata aatgtagtcg
    15001 cgcccaagct tgccccatga ggccagcagc gggttgccga catcctgttc accatcgctg
    15061 ttaaagagct gcccggcatt ttcgctgtcg cgaaatagcg gtaattctcg atcttcaaaa
    15121 ctgtgtcggc gctggcgagt cagtagtttc gccagataag caggatcttt aatgtcgccc
    15181 cagtaataac ggcaggggtt ggtaaacagg agatggattt caatatgttt acccagcgcc
    15241 tggagtgcct ggagataaac aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa
    15301 ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg ctgatagaga
    15361 ttggcgcggt gccagcgcgg ttgcccgagt tcatgggtat attccaccaa cgccttccac
    15421 aacggcgctt gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc tatttcccac
    15481 tgtgccagcc agtccggacg atagaccaga tactggtcaa acaggtccgc cgcttttgaa
    15541 gaaagctgga acagttttcg cttgtcgcta tcgtcagtca gataatgccg caacagggta
    15601 aagtcttcgc gctccaacag ttgcggcagc agagtcatca gtttccagct catgctctgt
    15661 ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc ggacgaacat atcccagata
    15721 aagctcgctg gcagcggaaa atcaatgttt gccgcaatac caaacttttg cgacagggtc
    15781 atttgcagcc actgcgccat accggtactt tgcaccagaa tcatttctgg ttcgaaagga
    15841 tcgtccagcc gttcgcgttc gacgataaac tccatcaacg cttccagcac gtccagacga
    15901 ttggaatggt agacccttaa catagcggct cctgactact gacgattcgg gcaatgcagg
    15961 cgggtcatct cgccttctct gcccccgggt gaaactagcg taacgctgat gctgacacat
    16021 cccgcctgcg atgtctgcat tcggttgacc tgccagttag caggtggcga aatcgcgcgc
    16081 agttgcgttt gctgccagcc atgccgccag agctgttggt actggtttct gctggcaaga
    16141 ctgttcatta atgttcgctg gaaacccgat aacgcagtga caatcatcac catcaacacc
    16201 atcgccaaca ttacctccgg cagactaaag ccttgttgat tcctcaggga agctgacata
    16261 acgccctctc tttcagcgga caaaaatcgc tccagccgcg tggcgaaaaa acaatgtttc
    16321 catcgatgac ttcgcctgtt cgccacaacg aaacgccttc ataaccagca atcaataagg
    16381 cttcattatc tgccagtaaa cgcagacaaa cccgggcacc ggtggcagca tacagtaagc
    16441 actgaactgc gggcagcgtc tgccaggagt gcatttttcc ccactccagc gccgactgaa
    16501 cgatggcctg gcggcgcaat gactgacttt ccatgctcac gcgagaagca aaactgcgat
    16561 cctgctgact cattccttgc aatagcaagc tacccaaaat cagcagcatc aggaccagag
    16621 ccagtgacga aacacccttt tcgcggttca caggttggat cctgtcacgc tatagctggc
    16681 atccaccacg gtttgcggat cagccttgct ggcagcgtgc atattaaccg tcaacaccgg
    16741 cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg tcgataatga tggcatccgg
    16801 attagtgact ttatcccagc ccttaccttc acaggatgtc gcaccgcgta gcgtttccag
    16861 cacatgctcc ttcagacgaa atccaatctg gtcggactct tttaccggtt cgcgatccca
    16921 gataccatta ctgttcgcat cccactgcac aatgacacag tcaccctgtc cgacaatttc
    16981 cagcccttcg cccgtacagc tgccatggca ataacccgcc ctctggagat gcttcgcgac
    17041 ggtaaatacc cgcagccaga tttcatcttc cagcgccagc ttacgggtgt tcgttaaact
    17101 ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc aacaatacgc tactgatcgc
    17161 catagcaatc aacacttcca gcagagaaaa accttgctct tttacaggca tccttctgtt
    17221 tctccttgct gacaaagccg gagtcttccc cacggcgaaa ccactagcca ccactcgccc
    17281 gttgagtttt tgaagcgaat atgcccggcc catgcggtat tgcgcaggcc aaagaaagca
    17341 agcgaaggtg tcaggtcgct catttcgact tcgggccagc gcggcacaaa gaccaatggt
    17401 gaactgccat gacaggtatt cgccccagca acggaactca caaggcacca taacgtcccc
    17461 tccctgataa gactgatact gtggtcgcgg ttatgccagt tggcatcttc acgtaaatag
    17521 agcaaatagt cccgcgcctg gctggcggtt tgccaaagcc gttgcgactg ctgccagtat
    17581 tgccagccat aaagcccact tgcgcttagc atgaccagaa tcagcatcgc gaccagcgtt
    17641 tcaatcagcg tataaccacg ttgtgttttc atgccggcag tatggagcga ggagaaaaaa
    17701 agacgagggc cagtttctat ttcttcggca catcttccgg actatttacg ccgttgcagg
    17761 acgttgcaaa atttcgggaa ggtgtctcga agaatttaac ggagggcaaa aaaaaccgac
    17821 gcacactggc gtcggctctg gcaggatgtt tcgtaattag atagccaccg gcgctttaat
    17881 gcccggatgt ggatcgtatc cttcaatctc aaagtcttcg aaacggtagt cgaagatgga
    17941 ttcgggttta cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag
    18001 atgagtttga tccatatggt tgctgtagag atgcgtgtcg ccaccggtcc agacaaaatc
    18061 acccacttcc agatcgcact gctgcgccat catatgcacc agtaacgcgt agctggcaat
    18121 gttgaacggc aggccgagga agacgtcaca ggagcgctga taaagctggc aagagagttt
    18181 gccgtctgcc acatagaact ggaagaatgc atggcacggt gccagcgcca ttttatccag
    18241 ttcgcctacg ttccacgctg aaacaataat gcggcgggaa tccgggtcgt ttttcagctg
    18301 gttcagtaca gtagtgatct ggtcaatatg acgaccatcc ggcgttggcc aggcgcgcca
    18361 ctgtttacca tacactggcc cgaggtcgcc gttttcatcg gcccattcgt cccagatggt
    18421 gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc tgcaggaacc acagcagttc
    18481 gtggatgatg gaacgcaggt ggcaacgttt agttgtcacc agcgggaatc catcctgcag
    18541 gttaaaacgc atctgatgac caaaaatgga aagcgttccg gttccggtac ggtcgttttt
    18601 ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa tactgtttca tggttcctca
    18661 ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc atcatgatca cacccgcgac
    18721 aatcatcggg atggaaagaa tttgccccat gctgatgtac tgcacccagg caccagtaaa
    18781 ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga aacgcgccgt aaccaatcag
    18841 gaacaagcct gagacagctc ccattgggcg cggtttacga atatacaggt tgaggataat
    18901 aaacagcacc acaccttcca gcagcagctc gtaaagctgt gatgggtggc gcggcagcac
    18961 accgtaagtg tcgaaaatgg attgccactg cggattggtt tgcagcagca aaatatcttc
    19021 tgtacgggag ccagggaaca gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa
    19081 ttcaccgtta ataaagttgc ccaggcgccc ggcaccaaga ccaaacggaa tgagtggggc
    19141 aataaaatca gagacctgga agaaggaacg tttagtacgg cgggcgaaga taatcatcac
    19201 cacgataacg ccaatcaggc ctccgtggaa ggacatgccg ccgtcccata cacggaaaag
    19261 atacagcgga tcggccataa actgcgggaa attgtagaac agaacataac caatacgtcc
    19321 accgaggaag acgccgagga agcccgcata gagtaagttt tcaacttcat ttttggtcca
    19381 gccactgccc ggacgattcg cccgtcgtgt tgccagccac attgcaaaaa tgaaacccac
    19441 cagatacatc aggccgtacc agtgaagcgc cacgggtcct attgagaaaa tgaccggatc
    19501 aaactccgga aaatgcagat agctactggt catctgtcac cacaagttct tgttatttcg
    19561 ctgaaagaga acagcgattg aaatgcacgc cgcaggtttc aggcgctcca aaggtgcgaa
    19621 taatagcaca aggggacctg gctggttgcc ggataccgtt aaaaaatatg tatatgatcc
    19681 gcgctataac cccccgcgaa tcaacccgcc catgccgcga cgctccataa aggctgcaac
    19741 ctgatggcga acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa gattttctgc
    19801 ttcagcaaaa tcaatgcgcc gcagcaggta ttttacgcgc gctacagaac gtccgttcat
    19861 agacagatgg cgatacccaa gcccaatgag gattgccacg cacatgggat cgcccgccat
    19921 ttcaccgcac aaacggagat cgattccatg tatttccgct tcccgggcga tcatcgccag
    19981 agctcgtaac attgcaggat gaagactgtc ataaatgttc gccacccggg tattgttgcg
    20041 atcaacggcc aggatgtatt gagtcagatc gttggtgcca acagagatga aatcgacccg
    20101 ctttgccaga tgcggcagca taaataccat tgacggcact tccagcatga tgccgatacg
    20161 tggtttggga atttcgtaac cgatcatctc ctcgacttca cgtccggcac gttcaatcag
    20221 gcggcgtgct tcgtcaactt catcgaggct tgtgaccatc ggcaacagaa tattcaggtt
    20281 gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg atcaagaaga tctccggctg
    20341 atcgagcgta atgcgaatcc cacgccaacc caggcatgga ttctcttcgc taatcggcat
    20401 gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt
    20461 aaacatttgc agcatcccct gatactgcgc cacctgttct tcttccgacg gaaaaccact
    20521 ttgcagcatg aatgggattt cagtgcgata aagtcctatg ccatcaatac ggctgcccag
    20581 tttttcttca tgttccgggc ttaaaccggc attgagcatg actttaatgc gctcgccgct
    20641 ttttaactgg gcgggtaaat tgacgtcatc ttccgccaga cggctaagct cgatctcttc
    20701 actaattagc cgctgatatt cttgcagcag taccggttcc ggatcgacca gcaattcacc
    20761 gcgataacca tcgacgatca gcgtccgacg atgcagtacc gaaggctgaa tatccgcgcc
    20821 catcacggta ggtatcccca gcgcacgtac catgatcgca gcatgggagt tggcagcgcc
    20881 atcgcgcacg acaacaccga ctaagcgatc ctggggcagc tcagcaagcg ttgtcgctga
    20941 cagttcatct gccaccagaa tgaaacgttc cggccaggcg ttcggccctt gattagcgtc
    21001 atcaagatga aacagcaatc gctgacccag cgcacgtaaa tcgccagccc gctctttgag
    21061 atagttatcg cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg tttttaccgc
    21121 ccactctgcc accgagcctt tatcaacctc ggcaaacaat tcgcgacgca gccgggtatc
    21181 cgaaagcagg tgtgagtaaa gatcgaaaat agccgccgtt tctttttgtg caccggcagc
    21241 aaagcgtttg ctatagcggc gaaactcgtt agccgcctct tccaacgccc cggtcagtcg
    21301 ttcgcgttcc agagccggat ccagcgttga tgcctgatac acctgttcca ttaaaggtaa
    21361 cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt gctgccggta atgcacggat
    21421 tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc gactgagaaa gaatagctgc
    21481 catctgggtg gcaagcgtca ccaggaagga ttcttcactt tcgtcatact ggcgcaactc
    21541 tcgttgctgt accaccagta caccaagcaa ctggcgacgt tgaataattg gtacgcctaa
    21601 aaacgcgcgg aaacgttctt cttttacgga ggggatgtat ttgaagctgg ggtgcttttg
    21661 cgcatctgca aggtttatcg gttccgccag cctgccaacc aggccgacga tcccttcatc
    21721 aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagt ccccgggtcg ccatcaggta
    21781 gtaacaacgt cgatcatgat cggccaggta gaccgaacag acctcggtat ccatcgcaag
    21841 acagatgtcg gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc tggctacctt
    21901 ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg acttaacctc ttttacgtcg
    21961 ataagcagat gcgttttgtg gttttggcgt attttcctgc agtgacatca ccacactcgc
    22021 gaactctttc attaccctac ggtagacatc acgtttaaat gacaccacct gtctgactgg
    22081 ataccagtaa cttacccatc gccagccgtc aaactctggt gtactgctgg tttgcatatt
    22141 gatttctgca tcgccgctca ccagctgcaa gagaaaccat ttttgttttt ggccgataca
    22201 aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca accagttacg
    22261 cgttgaagca aggattcgaa cgtctttgcg gcttaatcct acttcttcaa acaattcacg
    22321 gtacatcgcc tgctctgcgg attctccggg gttgattccg ccttgcggaa attgccagga
    22381 gtgctgacca aatcgccggg cccacattac ctgcccctgg cgattacaaa tcacgatacc
    22441 tacgtttggg cggtagccat cgtcatcaat caccggacta cctcaaaata aagctttata
    22501 tacgaatgat tgtttcatac tccaggaaga cggtaaacca ctctctgcag ggcattacac
    22561 actaataaca attgaataac tcacagttat gtgcagagtt ataaacagag gaaggggtgg
    22621 atagccgttt ttattcacct tttctgtgga tagagttgtg aagaactacg gaattactac
    22681 gggaaaaccc ggagcattct gaataagaca gatacagaga ttttaaaata ttcattatca
    22741 gtcaatgcat tacatgtgat ttgagtaata aaaaagagta attcgtgacc caggtcacac
    22801 ctctcattta cgggttggcg aaagatcaac caatgccgta tttatccaca gaatgtgcca
    22861 ctaagttaag cactgaacca ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg
    22921 gaaacagtag tgaaggtttt tcacagttat cccagctttc tgtggataac atggtgtaag
    22981 atcctgttta ttttcagtga ccagatttgg aaaacccgtt gcagtgttgc gcaactcgat
    23041 taccggcaac ctaaaaagca atataaatca gagaattgaa caacgcatgt ggaaaaagtt
    23101 acactgcgaa tattcggcac ttaattgctg tttgtttttt aaccaaggta tcatgacatg
    23161 tcccaacctc gcccactgct ctctcctccc gaaactgaag aacaattgtt agcgcaagca
    23221 caacaacttt ctggttatac attgggagaa ctggcggcac ttgccgggct ggttacgccg
    23281 gagaatttaa aacgcgataa gggctggatt ggcgtgttac tggagatctg gctaggtgcc
    23341 agcgcaggga gtaaacctga gcaagatttt gctgctctgg gcgtggaact taaaactatc
    23401 cctgtggata gtcttggtcg tccgctggaa acaacattcg tttgtgttgc cccgttaacg
    23461 ggcaatagcg gggtgacctg ggaaaccagc cacgtgcgcc acaagctcaa acgcgtgctg
    23521 tggataccgg ttgaaggcga gcgcagcatc ccgctggcaa agcgtcgcgt aggatcaccg
    23581 ttgctgtgga gcccgaatga agaggaagac cgacagttac gcgaagactg ggaagaatta
    23641 atggatatga ttgttctcgg tcagattgag cggatcaccg ctcgtcacgg ggaatattta
    23701 cagatacgac cgaaagcagc gaatgcgaaa gcgctgaccg aagctattgg tgcccggggc
    23761 gaacggattc tgacgctgcc gcgcggattt tatttgaaga agaatttcac cagtgcgcta
    23821 ctggcccgtc attttctgat ccagtagcca tcgctttgac ctgccgcttt ccgggcatat
    23881 aattaccgct tcattttttt ggcagggctt tttagatgtt atttgcatgg ataaccgatc
    23941 ctaacgcctg gcttgcgctc ggtacactga cgctgctgga gatcgttctt gggatcgaca
    24001 atattatttt cctttctctg gtggtggcaa agcttcccac agcacaacgt gctcatgcgc
    24061 gccgtctggg gttggcggga gccatggtca tgcgtctggc gctgctggca tccatcgcct
    24121 gggttacgcg cctgacgaat ccgcttttta caatattcag tcaggaaatt tccgcccgtg
    24181 atttgatcct gcttctgggt ggcttgttcc ttatctggaa agccagcaag gaaatccacg
    24241 aatccatcga aggtgaagaa gaagggctga aaacacgcgt ttcatcattc ctcggcgcta
    24301 tcgtgcagat tatgctgctg gatattatct tcagcctcga ctcggtgatt accgctgtgg
    24361 gtctgtcaga tcacctgttt attatgatgg ctgccgtggt gattgccgta ggcgtgatga
    24421 tgttcgccgc gcgctcgatt ggtgattttg tcgagcgcca tccttcggta aaaatgctgg
    24481 cgctctcttt cctgattctg gtgggcttta ccctgattct ggaaagtttc gacatccacg
    24541 taccgaaagg ttacatctac ttcgcgatgt tcttctctat tgcggttgaa agcctcaacc
    24601 tgattcgcaa caaaaagaat ccgctctgat actccgtacg ctctcctgcg ggagggcgta
    24661 ctcactcctg ttaccctcac agtttaagat tttcctgctt tcaagatata taacgtcggt
    24721 ttataaacag actattatca taggtgggcc aaatgaggat aaccggatga aaaaatgggc
    24781 agtaataatt tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca gtgattacgt
    24841 catggcgacc aaagatggcc gtatgatttt gaccgatgga aaacctgaaa ttgatgatga
    24901 taccgggctg gtgagttatc acgatcagca aggtaacgcg atgcaaatta accgtgatga
    24961 tgtttcgcaa attattgaac gttaacaaat aaggtcagca tccggctggc cttaagattt
    25021 ttctctttcc cttttccctt ccctctgcca tttttatatt ccttatgtcg tgattataaa
    25081 aaggaaacgg ctatgcaata tcaccgtata ccccacagtt cgctggaagt cagcacgctg
    25141 gggcttggca cgatgacgtt tggtgaacag aacagcgaag ccgacgccca cgcacaactc
    25201 gactatgccg tcgctcaggg cattaacctt atcgacgttg ccgaaatgta cccagtacct
    25261 ccgcgccccg aaactcaagg gctaaccgaa acctacgtcg gcaactggct ggcgaaacat
    25321 ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac
    25381 aaaggcatcc gcccggatca ggcgctggat cggaagaata tccgcgaagc gctgcatgac
    25441 agcctcaagc gtctgcagac tgattacctc gatctttatc aggtgcactg gccgcagcgc
    25501 ccaaccaact gcttcggcaa actcggttat agctggacag attctgcgcc tgcggtttcg
    25561 ctgctggata cactggacgc actggcagag taccaacgcg cgggaaaaat tcgttatatc
    25621 ggcgtgtcga acgaaactgc atttggcgta atgcgctacc tgcatctggc ggacaaacac
    25681 gatctgccgc gtattgtcac cattcagaac ccctacagtc tgttaaaccg cagttttgaa
    25741 gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg
    25801 ggtttcggca cgctgaccgg gaaatatctc aacggtgcaa aacccgctgg cgcacgtaat
    25861 acgctcttta gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg
    25921 tatgttgata tagccagacg tcatggcctg gatcctgcac agatggcgct cgcttttgta
    25981 cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga gcagctgaaa
    26041 actaacgtcg aaagtttgca tctggagtta agcgaagacg tattagcaga aattgaagcg
    26101 gtgcatcagg tttacactta tccggcacca taagattaac gccggttaaa accggcgtta
    26161 aatattaata acggcgctgc cagatccaca gcgccgttat tgccagcgca aacagcgcac
    26221 cgaagccaat gccaatgggc acgaccggga tgcctaccat taccgccagc gagtaaatgc
    26281 ccagcatcaa caacatggcg ctgttttcgc caaggttttg tactgcaatc gcattccccg
    26341 ccccgacgct ttttttaccc cgttcctgta gcaacgcatt gagcggaacg acaaaaaagc
    26401 cccccagcac gccaatcagc atcaacaagg cataggctga cagcagctcg tgttgcaggg
    26461 agaaaatcag taccaccacg ccaatcaaaa tcccggctgg catacagcgt gacacggttt
    26521 ccagcgtaac taacttcgct gccgcaccag cgccaaccac gataccaatc gctaccatcg
    26581 cgttgagata ggtcggcgta gcgttatcgg taatgcccag cgccaccggt acccacagca
    26641 ccaacaggaa acgcagcgtg acacccgctc cccagaataa actggtgccc accagcgaaa
    26701 aacgcgtttc accattgcgc catagcgagg tgcaggcatt caggaaactg cgggtcatgc
    26761 tgatgagatt ccaggactgc cccggacgcg ccgccgccag tttaggaatg tagatattgg
    26821 caacgaccgc accaccgtag gccagtgcgc atgcggccag ggcgaccagg acatgccagt
    26881 cagccagtac accaccggct acggaaccga gcaaaatcgc cgctatggta gaagcttcca
    26941 ttaaaccatt agctttcact aacttactac ccgtggttaa ttcgccgaga ataccgtatt
    27001 tcgccggtga ataggctgca gcaccaacac ccaccagcgt atagccgaga aacggattga
    27061 taccaaagca gatactggct gcgcccagca gcttcaggcc gttggcaaac atcatcaccc
    27121 ggcctttggc gaagctatcc gccacctgtc cgacaaacgg cgcaaaaaga atgtaagcac
    27181 ctacaaacac catttgcagg atgggctggc tccactccgg ataaaactgc gctttcagta
    27241 acgccagagt ggcaaacagc agcgcattat cgccaaacgc agagagaaac tgcgccacga
    27301 taaccgcttt catccccttc gaccacaacg aagtgttagt gtgcactgac tcactcatcg
    27361 tgttgttccg cttcgtctac ccagcttttc aacgtgacaa agtcaggttt gccgctgcca
    27421 agtaatggca tctgtttcag atagcgaata tcgcgcggta cagcaagctc cggcacgccg
    27481 tgctcgcggg catactgttg caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc
    27541 accagtgcct cgcctttgct ggcatcgctc ttaatcgcag tggcatggac tttatctggc
    27601 gaaacaccaa gtgccagttg ttccaccatt tccagcgaca ccatttcgcc tgcaattttg
    27661 gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct gctcgtcgaa acgcacaata
    27721 tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa cattctcggc ggtgggcact
    27781 tccagtacac ctggcttctc cacccgcaga tagccgttca ttatgttcgg ccctttcagt
    27841 tgcaggcgtc cgccctcttc gataccagga accgacaaca ggcgcgcatc cattcctggt
    27901 agaatacgcc ctaccgtacc gggtttcgcc gccatcggta cgttgataga aacgacaggc
    27961 gcgcactcgg tcacaccgta gccttcaagg atgcgcaggc caaatttatc ctgccaaagc
    28021 tgtttggtac tttcttgtag tttttctgcg cctgccacca catagcgtag acggtagaag
    28081 tcatacgggt tggcgaaacg cgcgtagtga ccgagaaaag tcgaggtacc gaacaacacg
    28141 gtgcaactgc ggtcatacac caactccggc acaatgcggt aatgcagcgg gcttggataa
    28201 aggaacacct ctgcacctgt gagcagtggc gtaaacaggc ctaccgtcag cccaaaggag
    28261 tgaaacagcg gtaacgccga cataaagcga tcgttggtgg tgaagtcggc aatcgtttta
    28321 atctgctcga cattcgccag aatgctttta tggctatgga cgacgccttt cggatggcct
    28381 tcagaaccgg aggtaaaaag gatcagcgcc tcttcttccg gctgctgttt tacctgtgcc
    28441 agacgcggca tcagcaaatg agcgaagatc catactttgt cggcagtggt gacatctgct
    28501 tttaaatctt ccagatagac ccagcgcacc tgagtaagtt gctccggcag atgccagagt
    28561 ttgcctttat cgagaaactg acgggaagtg aaaatggttt tgatttcagc cgccgtaata
    28621 gcactggtca acccttttac cccggcggtg tagttcatca ttgcgggaat gcggcgacgg
    28681 gcgatggccc caaaaatcac tgccgcactg atgcctgcat tgggcagcat taagccgatg
    28741 cgttcgcctt caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc
    28801 aatttgcgat aggagtccgg ggtaaagtta acgtcttcga cacatttctt cccggctccg
    28861 aagcggtaca ttgcgctcag taaagattcg tacagcgttt cacgcgggcg caccgccata
    28921 cgcgcttcca tcattatttg atgcagcatt tcgccagcga ttttgcgacg gtcacgggca
    28981 cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa tatgcagagt aatttgcggg
    29041 aacaagcgac gtttaaccag acctttcagg cggctgaagt gcgtaagttc cgccccttca
    29101 atacgcacag gaataaccgt tgcaccagac ttcgccgcga caaatcccgc gccatcgtag
    29161 attttcatca gcgagccagt ggtggtgatg cgtccttcag ggaaaatcac cactggtcgg
    29221 ccctgttcca ccagacgtac cagatgttta atcgccatag gttgcgtcgg gtcgagagga
    29281 acaaagtcga taaatgattt cagccaacgc atataccact gttggcttat tgaggtgtaa
    29341 acggcaaata ccggacgtac gggtaaaaac aatgccagca gtatgccatc aataaaagag
    29401 acgtgattag gcgtgattag aacgcgctcg cccttcagtg cctgggtgtc acccgtaacg
    29461 cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat tccaactccc
    29521 tttgctctga ttcagtaaaa gcgaatggag ggagattaca cgagataaag aacgcgagcg
    29581 acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc aagagacagg
    29641 gtacgaagag cgtaccgaat aatctcacca atcaatacct ctgggatctt gattgtggtc
    29701 tgcacgacga ctcttcgcca gtgagaaaac gcaaaggaat gaagggaaat gcaacgaggt
    29761 gtgtaaattg tcggttactg ttacagattg atgaccg
//
LOCUS       DAZGHF010000052        29338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGHF010000052 DAZGHF010000000
VERSION     DAZGHF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.052"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            441..1580
                     /gene="wza"
                     /locus_tag="ACHK3A_002387"
     CDS             441..1580
                     /gene="wza"
                     /locus_tag="ACHK3A_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO8740453.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1586..2029
                     /gene="wzb"
                     /locus_tag="ACHK3A_002388"
     CDS             1586..2029
                     /gene="wzb"
                     /locus_tag="ACHK3A_002388"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO8740454.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFVAVYTLLERSARQWAQALNAEQV"
     gene            2032..4194
                     /gene="wzc"
                     /locus_tag="ACHK3A_002389"
     CDS             2032..4194
                     /gene="wzc"
                     /locus_tag="ACHK3A_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO8740455.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGEITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4372..5211
                     /gene="wcaA"
                     /locus_tag="ACHK3A_002390"
     CDS             4372..5211
                     /gene="wcaA"
                     /locus_tag="ACHK3A_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO8740456.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5214..5702
                     /gene="wcaB"
                     /locus_tag="ACHK3A_002391"
     CDS             5214..5702
                     /gene="wcaB"
                     /locus_tag="ACHK3A_002391"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO8740457.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5699..6916
                     /gene="wcaC"
                     /locus_tag="ACHK3A_002392"
     CDS             5699..6916
                     /gene="wcaC"
                     /locus_tag="ACHK3A_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO8740458.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQRVAGKRQLFSEMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQSSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6891..8108
                     /gene="wcaD"
                     /locus_tag="ACHK3A_002393"
     CDS             6891..8108
                     /gene="wcaD"
                     /locus_tag="ACHK3A_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO8740459.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8119..8865
                     /gene="wcaE"
                     /locus_tag="ACHK3A_002394"
     CDS             8119..8865
                     /gene="wcaE"
                     /locus_tag="ACHK3A_002394"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO8740460.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAHGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8881..9429
                     /gene="wcaF"
                     /locus_tag="ACHK3A_002395"
     CDS             8881..9429
                     /gene="wcaF"
                     /locus_tag="ACHK3A_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO8740461.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9456..10577
                     /gene="gmd"
                     /locus_tag="ACHK3A_002396"
     CDS             9456..10577
                     /gene="gmd"
                     /locus_tag="ACHK3A_002396"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO8740462.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYDMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIAIGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10580..11545
                     /gene="fcl"
                     /locus_tag="ACHK3A_002397"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10580..11545
                     /gene="fcl"
                     /locus_tag="ACHK3A_002397"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO8740463.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11548..12027
                     /gene="gmm"
                     /locus_tag="ACHK3A_002398"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11548..12027
                     /gene="gmm"
                     /locus_tag="ACHK3A_002398"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO8740464.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVVEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            12024..13247
                     /gene="wcaI"
                     /locus_tag="ACHK3A_002399"
     CDS             12024..13247
                     /gene="wcaI"
                     /locus_tag="ACHK3A_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO8740465.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWQPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVANADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13250..14686
                     /gene="cpsB"
                     /locus_tag="ACHK3A_002400"
     CDS             13250..14686
                     /gene="cpsB"
                     /locus_tag="ACHK3A_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO8740466.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRTAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAIDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14879..16249
                     /gene="cpsG"
                     /locus_tag="ACHK3A_002401"
                     /gene_synonym="manB"
     CDS             14879..16249
                     /gene="cpsG"
                     /locus_tag="ACHK3A_002401"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO8740467.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     NNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLADPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16304..17698
                     /gene="wcaJ"
                     /locus_tag="ACHK3A_002402"
     CDS             16304..17698
                     /gene="wcaJ"
                     /locus_tag="ACHK3A_002402"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO8740468.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17700..19178
                     /gene="wzxC"
                     /locus_tag="ACHK3A_002403"
     CDS             17700..19178
                     /gene="wzxC"
                     /locus_tag="ACHK3A_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO8740469.1"
                     /translation="MNLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDTI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLIVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19839..21119
                     /gene="wcaK"
                     /locus_tag="ACHK3A_002404"
     CDS             19839..21119
                     /gene="wcaK"
                     /locus_tag="ACHK3A_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO8740470.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            21116..22336
                     /gene="wcaL"
                     /locus_tag="ACHK3A_002405"
     CDS             21116..22336
                     /gene="wcaL"
                     /locus_tag="ACHK3A_002405"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO8740471.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIIALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLCGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            22347..23741
                     /gene="wcaM"
                     /locus_tag="ACHK3A_002406"
     CDS             22347..23741
                     /gene="wcaM"
                     /locus_tag="ACHK3A_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO8740472.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            23899..24894
                     /locus_tag="ACHK3A_002407"
     CDS             23899..24894
                     /locus_tag="ACHK3A_002407"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO8740473.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLTAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            25137..26030
                     /gene="galF"
                     /locus_tag="ACHK3A_002408"
     CDS             25137..26030
                     /gene="galF"
                     /locus_tag="ACHK3A_002408"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO8740474.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            26402..27487
                     /gene="rfbB"
                     /locus_tag="ACHK3A_002409"
     CDS             26402..27487
                     /gene="rfbB"
                     /locus_tag="ACHK3A_002409"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO8740475.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            27487..28386
                     /gene="rfbD"
                     /locus_tag="ACHK3A_002410"
     CDS             27487..28386
                     /gene="rfbD"
                     /locus_tag="ACHK3A_002410"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO8740476.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEQCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVATTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTVI"
     gene            28444..29322
                     /gene="rfbA"
                     /locus_tag="ACHK3A_002411"
     CDS             28444..29322
                     /gene="rfbA"
                     /locus_tag="ACHK3A_002411"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO8740477.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFHQLLGDGGQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKKGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGGLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLRMIKGY"
BASE COUNT         7329 a         7115 c         7866 g         7028 t
ORIGIN      
        1 taaaaaccat attgaatgac acttaatata attcttaaaa atagccaatt gccgaattgt
       61 taccttgcct gctattccgt tagctgtaac acttcctcct gcattattgg agagccaata
      121 ttcaattacc ataatgtcac cttatattcg gaagttaatt tccgcaataa ccctttctca
      181 tcaacgactg tatgttgaga gggttttaaa aattaagcta aatcagtgta ttggtagcta
      241 aaaagccagg ggcggtagcg tgtctggatg cctgaaagac cagtctgaat tattctgtca
      301 atagcctgcg gatgaaatca atttttttag gactgatgcc agttaaattt ttattcaatt
      361 aactgcaagg taaatgtgca tggcgacatt gctattaata gtgcacagga taattactct
      421 gccaaagtga taaataaaca atgatgaaat ccaaaatgaa attgatgcca ttattggtgt
      481 cagtaacctt gataagcggt tgcacagtac ttccgggcag caatatgtcg acgatgggta
      541 aagacgtcat caaacagcag gacgctgatt tcgatctcga caaaatggtg aatgtttatc
      601 cgttgacccc gcgcctgatt gatcaattac gcccacgccc gaatgtagcg cgccccaata
      661 tgacgctgga aagtgagatc gcgaattatc agtatcgcgt cgggccgggg gacgttctta
      721 atgtcaccgt ctgggatcac ccggaactca ccacgccagc cggtcagtac cgcagctcca
      781 gcgacaccgg caactgggta cagcctgacg gcaccatgtt ctacccgtat atcggcaagg
      841 ttcacgtcgt ggggaaaacg ctcgctgaaa tccgcagtga tattaccggt cgcttagcga
      901 cgtacatcgc tgacccgcag gtggacgtta atatcgccgc cttccgctcg caaaaagcct
      961 atatctcagg tcaggtgaat aaatccggtc aacaggcgat taccaacgta ccactgacca
     1021 ttctcgatgc catcaacgcc gcaggtggcc tgaccgacac cgctgactgg cgcaacgtgg
     1081 tgctaacaca caatggtcgt gaagaacgca tttctttgca ggcgctgatg caaaacggcg
     1141 acctcaatca gaaccgactg ctttaccccg gcgatattct ctacgtgccg cgtaatgatg
     1201 atctgaaagt gtttgtgatg ggtgaagtta aaaaacagag caccctgaaa atggacttta
     1261 gcggcatgac cctaactgaa gccctgggta atgctgaagg catcgacatg accacctcca
     1321 acgccagcgg catctttgtc attcgtccgt tgaaaggcga gggcgggcgt aacggcaaga
     1381 ttgccaatat ctaccagctg gatatgtccg atgccacatc gctggtgatg gcgacagaat
     1441 tccgcctgca accttatgac gtggtgtatg tcaccaccgc cccggtttcc cgctggaacc
     1501 gtctgatcaa tcagttgctg ccaactatta gcggtgtccg ttacatgacg gatacagcca
     1561 gcgacattca taactggtaa tcgtcatgtt taacaacatc ttagttgtct gtgtcggcaa
     1621 tatttgccgt tccccgacgg cggaacgctt actgcaacgt tatcacccgg agctgaaagt
     1681 ggagtccgct ggactcggcg cgctggtcgg taagggcgct gaccctactg ctatcagcgt
     1741 cgccgcagaa catcaactgt ctctggaagg tcactgtgca cggcaaatca gccgtcgcct
     1801 gtgtcgcaac tacgacctga ttttgaccat ggaaaagcgc catatcgaac gcttatgcga
     1861 gatggcaccg gagatgcgcg gcaaagtgat gctgtttggt cactgggata acgaatgtga
     1921 aatccccgat ccgtatcgca aaagccggga aacgtttgta gcggtgtaca cattacttga
     1981 acggtctgcc cgccagtggg cgcaggcatt gaacgcagag caggtataag aatgacagaa
     2041 aaagtaaaac aacatgccgc tccggtaacg ggcagtgatg aaatcgatat tggtcgcctg
     2101 gtcggcaccg tcattgaagc gcgctggtgg gtgattggca tcaccgccgt atttgccctc
     2161 tgtgccgtgg tttacacctt cttcgctacg ccgatataca gcgccgacgc actggtacaa
     2221 atcgaacaaa gcagcggcaa ttcgttagtg caggatatcg gatcggcatt agccaacaaa
     2281 ccgcctgcat cggacgccga gatccagttg attcgttcgc gcctggtgct tggtaaaacg
     2341 gtggacgatc tcgacctcga tattgcggtg agcaaaaaca cgttcccgat tttcggtgcg
     2401 ggctgggatc gcctgatggg acgccagaac gagacggtaa aagtgaccac ctttaaccgt
     2461 cctaaggaga tggcggatca ggtgtttacg cttaatgtgc ttgatgacaa aaactacact
     2521 ttgagcagcg atggcggctt tagcgcccgt gggcaagcgg gccagatgct gaaaaaagaa
     2581 ggcgtcacgc tgatggttga agctattcac gccagcccgg gcagtgagtt taccgtcacc
     2641 aaatactcca cgctggggat gatcaatcaa ctgcaaaaca gcctgacggt aacggagaac
     2701 ggcaaagatg ctggcgtttt gagcctgact tatactggag aagatcgcga acagatccgc
     2761 gacattctta acagtatcgc ccgtaactat caggaacaaa atattgagcg caaatcggcg
     2821 gaagcgtcga aaagcctcgc tttcctcgcg caacagttac cggaagtacg tagccgcctc
     2881 gatgttgccg aaaacaaact gaatgccttc cgtcaggata aagattcagt tgatctgccg
     2941 ctggaagcga aagcggtgct cgattcgatg gtgaacatcg acgcgcagtt gaacgaactg
     3001 acctttaaag aagcggaaat ctccaagctg tacaccaaag ttcacccggc gtatcgcacg
     3061 ctgctggaga aacggcaggc gctggaagac gaaaaagcca aacttaatgg tcgcgtaacg
     3121 gcgatgccga aaacccagca ggaaattgtc cgtctgaccc gcgatgtcga gtctgggcag
     3181 caggtctata tgcaactgct gaataaagag caggagctga aaatcaccga agccagcacc
     3241 gtcggcgatg tgcgcattgt tgacccggca atcacccagc ctggtgtgct aaaaccgaag
     3301 aaagggctga ttatccttgg ggcgattatc cttggcctga tgctctctat cgtgggtgtg
     3361 ctgctgcgct cgttgtttaa tcgcggtatc gaaagtccgc aggtgctgga agaacacggt
     3421 atcagcgtct atgccagcat cccgctgtcg gagtggcaga aagcgcgcga tagcgtcaaa
     3481 accatcaaag ggattaaacg ctataaacag agccagctac tggcggtggg gaatccaacc
     3541 gatctggcga ttgaagccat ccgtagtctg cgcaccagtc tgcacttcgc gatgatgcag
     3601 gcacagaaca atgtgttgat gatgaccggg gttagcccgt caatcggtaa aacctttgtc
     3661 tgcgccaacc tggcggcggt gatcagtcag accaataaac gggtgttgtt gatcgactgc
     3721 gatatgcgca aaggctacac ccacgagctg ttgggcacta ataacgtcaa tggcctgtcg
     3781 gaaattctga ttggtcaggg cgagattact accgctgcta aaccgacctc tattgccaaa
     3841 tttgacctga tcccgcgcgg tcaggtaccg ccaaatcctt cggaactgtt gatgagcgaa
     3901 cgttttgccg aactggtgaa ctgggcgagt aaaaactacg acctggtgtt gattgatacg
     3961 ccgccgattc tggcagtgac cgatgcggca attgttggtc gtcatgtcgg aaccacgttg
     4021 atggtggcgc gttatgcggt caacacattg aaagaagtgg aaaccagtct gagccgcttt
     4081 gagcaaaacg gtattccggt gaaaggggtg attctgaact ccatcttccg tcgcgccagc
     4141 gcgtatcagg attatggcta ttacgaatac gaatataagt cggacgcgaa ataacgaaat
     4201 ccgctgtcgg atgcgatgct gacgcatctt atccgaccta cgttcacgcc gcatcaggca
     4261 agcaaacccg ctccgtaggt cggataaggc gttcacgccg catccggcaa cagaacctgt
     4321 aaaccagctt ataaaccggc agaacatctc gacactccgg gagaacaacc tatgaaagac
     4381 aatccgctga tttcaatcta tatgccgacc tggaaccgtc aacaactggc gattcgggcg
     4441 ataaaatcgg tcctgcgcca ggactacagt aactgggaga tgatcatcgt ggatgattgc
     4501 tccacctcct gggaacaact acaacagtac gtcaccgccc tcaacgatcc gcgtatcact
     4561 tacattcaca acgacattaa cagcggggcg tgcgcggtac gtaaccaggc gattatgctg
     4621 gcgcaagggg aatatatcac cgggatcgat gatgatgatg aatggacacc caaccgtctg
     4681 agcgtcttcc tcgcccataa acagcaactg gtcacacacg cctttttgta cgctaacgac
     4741 tacgtttgcc agggcgaagt ctattcccaa ccggcaagtt tgccactgta tcccaaatcg
     4801 ccgtattcac gccgcctgtt ctacaaacgc aatatcattg gcaatcaggt ctttacctgg
     4861 gcgtggcgtt tcaaagagtg tttgttcgat accgaactga aagcggcaca ggattacgac
     4921 attttcctgc ggatggtggt ggagtatggc gaaccgtgga aagtggaaga ggcgacgcag
     4981 atcctgcata tcaatcacgg cgaaatgcag atcacctcgt caccgaaaaa attctccgga
     5041 tacttccact tttaccgtaa gcacaaagac aaatttgacc gcgccagcaa aaaatatcag
     5101 ctgtttaccc tctaccagat ccgcaataaa cgcatgacct ggcgcacctt gttgacgctg
     5161 ctgtcggtgc gtaacggcaa acgtctggct gatggtattc gggggcgcta aagatgctgg
     5221 aagatctgcg cgccaacagc tggagtttac gcccgtgctg catggttctt gcctatcgcg
     5281 ccgctcattt ttgttcggtg tggcgcaaaa agaacgtcct caacaatctt tgggcggccc
     5341 cgctgctggt gctgtatcgc attatcaccg aatgcttttt cggttatgaa atccaggccg
     5401 ccgcgaccat tggccgccgt ttcactatcc atcacggtta cgccgtagtg atcaataaaa
     5461 acgtggtagc gggggatgat ttcaccattc gccacggcgt cactatcggc aatcgtggtg
     5521 ccgataacat ggcatgtcca cacattggca acggcgtcga actcggtgcc aacgtcatta
     5581 ttcttggtga tatcacgctt ggtaacaacg tcaccgtggg cgcgggcagc gtggtgctcg
     5641 actctgtccc ggacaacgcg ctggtggtgg gagaaaaagc gcgagtgaag gtaattaaat
     5701 gaatattctg caattcaatg tgcgactggc ggaaggcggg gcggcaggtg tggcgttaga
     5761 tctccaccag cgcgcgctgc aacaggggct ggcgtcacat tttgtctacg gctacggcaa
     5821 aggcggcaaa gagagcgtca gccatcaaaa ctatccgcag gtcatcaaac atacgccgcg
     5881 gatgaccgcg atggcgaaca ttgcgttgtt tcgtctgctt aatcgcgatc tgttcggcaa
     5941 tttcaatgag ttatatcgca ccattactcg cacaccgggt ccggtggtcc tgcattttca
     6001 tgtgctgcac agctactggt tgaatcttaa gagcgtggtg cgcttttgcg aaaaagtgaa
     6061 aaaccataaa ccggatgtca ctctggtctg gacgttacac gatcactgga gcgttaccgg
     6121 acgctgcgcc tttaccgacg gttgcgaagg ctggaaaacg ggctgccaga aatgcccgac
     6181 cttaaataat tatccgccgg tgaagattga tcgcgcacac caacgggtgg cgggcaaacg
     6241 gcagttattc agcgagatgc tggcgctggg ctgtcagttt atttccccca gccagcatgt
     6301 ggcagacgct ttcaatagtc tgtacggtcc agggcgttgc cggattatca ataacggtat
     6361 tgatatggca accgaagcga ttctggcgga cttgcctccg gtgcgcgaaa cccagggcaa
     6421 gccgaaaatc gcggtggtgg cgcatgacct gcgttacgac ggcaaaacta accagcaact
     6481 ggtacgcgag atgatggcgc tgggcgacaa aatcgaactg catactttcg gtaagttctc
     6541 gccgttcacc gctggtaacg ttgttaatca cggctttgaa actgacaaac gtaaactgat
     6601 gagcgcgctc aaccagatgg atgcgctggt gttcagttct cgcgtcgata actacccgct
     6661 gattttgtgt gaggcgttat cgattggcgt gccggtgatt gccacccata gcgacgcggc
     6721 gcgggaagtg ctgcaaaaat ccggcggtaa aaccgtcagc gaagaagatg tgctgcaact
     6781 ggtgcagtca agcaaaccgg aaatcgcaca ggcgatattt ggtaccacgc tggctgagtt
     6841 cagccagcgc agccgcgccg cctacagtgg acaacagatg ctggaggagt atgtcaactt
     6901 ctatcagaat ctgtagctac ctgctgctgc cgctgatcta cctgctggtt aacgtcaaaa
     6961 tcgcccagct tggcgaaagt tttcccatca ccatcgtcac ttttttacct gtcttgttac
     7021 tgctgttttt agaacgcatc agcgttaaaa aattgatgat tgccttaggg attggtgcgg
     7081 gactcacggc gtttaactac ctgtttggtc agtcgttgga tgccggtaaa tacgtcactt
     7141 caactatgct gtttgtctat attgtgatca ttattggcat ggtgtggagt attcgcttta
     7201 aaacgatttc gccccacaac caccgcaaga tattacgttt cttttatctg gtggtcgggc
     7261 tggtggtggt gctggcggcg gtggagatgg cgcaaattat cctcaccggc ggtagcagta
     7321 ttatggagtc gatttcgaaa tatctgattt acagcaacag ctatgtgctg aatttcatta
     7381 aattcggcgg caagcgcacg acagcacttt atttcgaacc ggcatttttc gctctggcat
     7441 taatctcaat ttggctcagc atcaaacagt ttggtatcaa aacgcctaaa accgatgcta
     7501 tgattcttgc agggataata ttatccggat cgttttcagg ggttatgacc tttatcctgt
     7561 tttatttgct ggagtgggca tttcaatatc tgaataaaga ggcgattaag aaaaagttac
     7621 cgttagcatt gatttctctg gctgtattcc tggttggtgt ggtaatcgcg tttccttata
     7681 tttccacccg tctgggcgat ttaggtacgg aaggatcgtc atcgtattat cgtattgtcg
     7741 gtccgctggt gatggtcggt tattctttga cccatattga cggtgtagtc agatttggct
     7801 cactttatga atatgtcgca tcattcggaa tatttaacgg tgcggatgtc ggaaaaacca
     7861 tagacaatgg tttgtatctg ctgattattt atttttcctg gttcgcagtg tttttatcgc
     7921 tgtggtacat ggggaaagtg ataaaaatga tgatcaacgc ttttggtgat aaccgcaatt
     7981 ttcgcgtgca attatatctt tttactccgg tatcgctgtt ttttaccggt tcgatattta
     8041 gcccggaata tgcattttta atcgtctgtc cgtttatttt gcgaaaagcg ttaaatatta
     8101 cgaggtaaga ataagaacat gttgcttagc ataatcactg tcgcgtttcg taacctcgca
     8161 gggatagtca aaacacatgc ctcgttggcg catctggcgc aggctgacga tatcagcttc
     8221 gaatggattg ttgtcgatgg cggttccaat gacggcaccc gtgagtatct ggaaaatctc
     8281 aatggtatct ataacctacg ctttgtcagc gagccagata acggtatcta cgacgccatg
     8341 aacaaaggca ttgcgatggc gcatggcaag ttcgcgttgt ttctcaactc aggcgatatt
     8401 tttcatcagg atgccgcaaa ttttgtccgt aagttaaaag tgcaaaaaga taacatgatg
     8461 atcaccggcg atgcgctgct ggattttggc gacgggcata aaattaaacg tagcgccaaa
     8521 ccgggttggt atatttatca cagcctgccc gccagtcatc aggcgatatt tttcccggtg
     8581 tccggcttga aaaaatggcg ttatgacctg gaatataaag tttcttccga ctacgcgctg
     8641 gcagccaaaa tgtataaagc cggttatgca tttaaaaaac tcaatggcct ggtgtctgaa
     8701 ttttctatgg gtggggtatc aaccaccaat aatatggaat tgtgtgctga cgccaaaaaa
     8761 gtccaacggc aaatattaca tgtgcctggc ttctgggcag aattatcctg gcatttacgc
     8821 caacgtacta cctcaaagac gaaagcctta tataacaaaa gctgaatata agggaaaacg
     8881 atgcaagatt taagcggttt ctcggtaccg aaagggttcc ggggtggcaa cgctattaaa
     8941 gtgcaattgt ggtgggcagt acaggcaaca atatttgcct ggtcgccaca agtattgtat
     9001 cgctggcggg catttttatt acgtttattc ggcgcaaaaa taggaaaaaa cgtagttatt
     9061 cgtccgtcag taaaaattac ctatccgtgg aaattaacct taggtgatta cgcgtgggtc
     9121 ggcgatgacg tcaatttata taccctcggt gaaataacca ttggcgcaca ttcggtgata
     9181 tcgcaaaaaa gttatttatg caccggtagc cacgaccatg caagtcaaca tttcaccatt
     9241 aacgccacgc ctattgtgat tggcgagaaa tgctggctgg caaccgatgt ctttgttgcc
     9301 ccaggcgtca cgatcggcaa cggcaccgtc gtgggtgcac gaagcagtgt ttttaaatcg
     9361 cttccggcaa atgtggtttg ccgggggaat cccgcagtgg tgatacgcga acgcgttgaa
     9421 actgaataaa ttcaaaaaat acagaggaat aagacatgtc aaaagtcgct ctcatcaccg
     9481 gtgtaaccgg acaagacggt tcttacctgg cagagtttct gctggaaaaa ggttacgagg
     9541 tgcatggtat taagcgtcgt gcatcgtcat tcaacaccga gcgcgtggat cacatttatc
     9601 aggatccgca cacctgcaac ccgaaattcc atctgcatta tggcgacctg agtgatacct
     9661 ccaacctgac gcgtattttg cgtgaagtgc agccggatga agtgtacaac ctgggcgcaa
     9721 tgagccacgt tgcggtttct tttgagtcac cggaatatac cgcagacgtc gatgcgatgg
     9781 gtacgctgcg cctgctggag gcgatccgct tcctcggtct ggaaaagaaa acccgtttct
     9841 atcaggcttc tacttctgaa ctgtacggtc tggtgcagga aattccgcag aaagagacca
     9901 cgccgttcta cccacgctct ccgtatgcgg tcgccaaact gtacgcctac tggatcaccg
     9961 ttaactaccg cgaatcctac gacatgtacg cctgtaacgg tattctcttc aaccatgaat
    10021 ccccgcgccg tggcgagact ttcgttaccc gcaaaatcac ccgcgcaatc gccaacatcg
    10081 cccaggggct ggagtcgtgc ctgtatctcg gcaatatgga ttccctgcgt gactggggcc
    10141 acgccaaaga ctacgtaaaa atgcagtgga tgatgctgca acaggaacaa ccggaagatt
    10201 tcgttatcgc gatcggcgtt cagtactccg tacgtcagtt cgtggaaatg gcggcagcac
    10261 agttgggcat caaactgcgc tttgaaggca cgggtgttga agagaagggc attgtggttt
    10321 ccgtcaccgg gcatgacgcg ccgggcgtta aaccgggcga tgtgattatc gccgttgacc
    10381 cgcgttactt ccgtccggcg gaagttgaaa cgctgctcgg cgacccgacc aaagcgcacg
    10441 aaaaactggg ctggaaaccg gaaatcaccc tcagagagat ggtgtctgaa atggtggcta
    10501 atgacctcga agcggcgaaa aaacactctc tgctgaaatc tcacggctac gacgtggcga
    10561 tcgcgctgga gtcataagca tgagtaaaca acgcattttt attgctggcc atcgcgggat
    10621 ggtcggttcc gccatcaggc ggcagctcga acagcgcggt gatgtagagc tggtattacg
    10681 cacccgcgac gagctgaacc tgctggacag ccgcgcggtg catgatttct ttgccagcga
    10741 aagcattgac caggtctatc tggcggcggc gaaagtgggc ggcattgttg ccaacaacac
    10801 gtatccggcg gatttcatct accagaacat gatgattgag agcaacatca ttcacgccgc
    10861 gcatcagaac gacgtgaaca aactgctgtt tctcggatcg tcctgcatct acccgaaact
    10921 ggcaaaacag ccgatggcag aaagcgagtt gttgcagggc acgctggagc cgactaacga
    10981 gccttatgct attgccaaaa tcgccgggat caaactgtgc gaatcttaca atcgccagta
    11041 cggacgcgat taccgctcag tcatgccgac caacctgtac gggccgcatg acaacttcca
    11101 cccgagtaac tcccatgtga tacctgcatt gctgcgtcgc ttccacgagg cgacggcaca
    11161 gaatgcgccg gacgtggtgg tatggggcag cggtacaccg atgcgtgaat tcctgcacgt
    11221 cgatgatatg gcggcggcga gcattcatgt catggagctg gcgcacgaag tctggctgga
    11281 aaacacccag ccgatgctgt cgcacattaa cgtcggcacg ggcgttgact gcactatccg
    11341 cgagctggcg caaaccatcg ccaaagtggt gggttacaaa ggtcgggtgg tttttgatgc
    11401 cagtaaaccg gatggtacac cgcgcaaact gctggatgtg acgcgcctgc atcagcttgg
    11461 ctggtatcac gaaatctcac tggaagcggg gcttgccagc acttaccagt ggttccttga
    11521 gaatcaagac cgctttcggg ggtaatgatg tttttacgtc aggaagactt tgccacggta
    11581 gtgcgctcca caccgcttgt ctctctcgac tttattgtcg agaacagtcg cggcgagttt
    11641 ctgcttggca aaagaaccaa ccgcccggcg cagggttact ggtttgtgcc gggagggcgc
    11701 gtgcagaaag acgaaacgct ggaagccgca tttgagcggc tgacgatggc ggaactgggg
    11761 ctgcgtctgc cgataacagc aggccagttt tacggtgtct ggcagcactt ttatgacgat
    11821 aacttctccg gcacggattt caccactcac tatgtggtgc tcggttttcg cttcagagta
    11881 gtggaagaag agctgttact gccggatgag cagcatgacg attaccgctg gctgacgccg
    11941 gacgcactgc tcgccagtga caatgttcac gctaacagcc gcgcctattt tctcgctgag
    12001 aagcgtgccg gagtacccgg attatgaaaa tactggtcta cggcattaac tactcgccgg
    12061 agttaaccgg catcggcaaa tacaccggcg agatggtgga atggcttgcg gcgcaaggtc
    12121 atgaggtgcg ggttattacc gcaccgcctt actacccgca atggcaggtg ggcgagaact
    12181 attccgcctg gcggtacaaa cgagaagagg gggccgccac ggtgtggcgc tgcccgctgt
    12241 acgtgccaaa acagccgagc accctgaaac gcctgttgca tctcggcagt tttgccgtca
    12301 gcagtttttt cccgttgatg gcgcaacgtc gctggcagcc ggatcgcatt attggcgtgg
    12361 tgccaacgct gttttgcacg ccgggaatgc gcctgctggc gaaactctct ggggcgcgca
    12421 cggtgctcca tattcaggat tacgaagtgg acgccatgct ggggctgggc cttgccggaa
    12481 aaggtaaagg cggcaaagtg gcacagctgg caacggcgtt cgaacgtagc ggactgcata
    12541 acgtcgataa cgtctccacg atttcgcgtt cgatgatgaa taaagctatc gaaaaaggcg
    12601 tggcggcgga aaacgtcatc ttcttcccca actggtcgga aatcgcccgt tttcagcatg
    12661 ttgcaaatgc cgatgttgat gcccttcgta accagcttgg cctgccggat aacaaaaaaa
    12721 tcattcttta ctccggcaat attggtgaaa agcaggggct ggaaaacgtt attgaagctg
    12781 ccgatcgcct gcgcgatgaa ccgctgattt ttgccattgt cgggcagggc ggcggcaaag
    12841 cgcggctgga aaaaatggcg cagcagcgtg gactgcgcaa catgcaattt tttccgctgc
    12901 aatcgtatga cgctttaccc gcactgctga agatgggcga ttgccatctg gtggtgcaaa
    12961 aacgcggcgc ggcagatgcc gtattgccgt cgaaactgac caatattctg gcggtaggcg
    13021 gtaacgcggt gattaccgca gaagcccaca ccgaactggg acagctttgc gaaacctttc
    13081 cgggcattgc ggtttgcgtg gaaccggaat cggtcgaggc gctggtggcg gggattcgtc
    13141 aggcgctcct gctgcccaaa cacaacacgg tggcacgtga atatgccgaa cgcacgctcg
    13201 ataaagagaa cgtgttacgt caatttataa atgatattcg gggataatta tggcgcagtc
    13261 gaaactctat ccagttgtga tggcaggtgg ctccggtagc cgcttatggc cgctttcccg
    13321 cgtactttac cccaagcagt ttttatgcct gaaaggcgat ctcaccatgc tgcaaaccac
    13381 catctgccgc ctgaacggcg tggagtgcga aagcccggtg gtgatttgca atgagcagca
    13441 ccgctttatt gtcgcggaac agctgcgtca actgaacaaa ctcaccgaga atattattct
    13501 cgaaccggca gggcgtaaca ctgcacctgc cattgcgctg gcggcgctgg cggcaaaacg
    13561 tcatagcccg gagagcgacc cattaatgct ggtgctggcg gcggatcatg tgattgccga
    13621 tgaagacgct tttcgcaccg ccgtgcgtaa tgccatgccg tatgccgaag cgggcaagct
    13681 ggtgaccttc ggcattgtgc cggatctacc tgaaaccgga tatggctata ttcgtcgcgg
    13741 tgaagtgtct gcgggtgagc aggatacggt ggcctttgaa gtggcgcagt ttgtcgaaaa
    13801 accgaatctg gaaaccgcgc aggcctatgt ggcaagcggc gaatattact ggaacagcgg
    13861 tatgtttctg ttccgcgccg gacgctatct cgaagaactg aaaaaatatc gcccggatat
    13921 tctcgacgcc tgtgaaaaag cgatgagcgc catcgatccg gatctcgatt ttattcgtgt
    13981 ggatgaagaa gcgtttctcg cctgcccgga agagtcggtg gattacgcag tcatggaacg
    14041 tacggcagat gctgttgtgg tgccgatgga tgcgggctgg agcgatgtcg gttcctggtc
    14101 ctctttatgg gagatcagcg cccacaccgc cgagggcaac gtttgccacg gcgatgtgat
    14161 taatcacaaa actgaaaaca gctatgtgta tgccgaatct ggcctggtca ccaccgtcgg
    14221 ggtgaaagat ctggtggtgg tgcagaccaa agatgcggtg ctgattgccg accgtaacgc
    14281 ggtgcaggat gtgaaaaaag tggtcgagca gatcaaagcc gatggtcgcc atgagcatcg
    14341 ggtgcatcgc gaagtgtatc gtccgtgggg caaatatgac tctatcgacg cgggcgaccg
    14401 ctaccaggtg aaacgcatca ccgtgaaacc gggcgaaggc ttgtcagtac agatgcacca
    14461 tcaccgcgcg gaacactggg tagtggtcgc gggaacggca aaagtcacta tcgacggtga
    14521 tatcaaactc cttggtgaaa acgagtccat ttatattccg ctgggggcga cgcattgcct
    14581 ggaaaacccg gggaaaattc cgctcgattt aattgaagtg cgctccggct cttatcttga
    14641 agaggatgat gtggtgcgct tcgcggatcg ctacggacgg gtgtaaacgt cgcatcaggc
    14701 gctgtttgtc ggatgcggcg tgaacgcctt atccgaccta cggttcgggt ttgtaggcct
    14761 gataagacgc ggcagcgtcg catcaggcaa tgaatgcgaa accgcgatgt aaataacgac
    14821 aaaaacaaaa ttggccgttt cggtcagggc caactattgc ctgaaaaagg gtaacgatat
    14881 gaaaaaatta acctgcttta aagcctatga tattcgcggg aaattaggcg aagaactgaa
    14941 tgaagatatc gcctggcgca ttggtcgcgc ctatggcgaa tttctcaaac cgaaaaccat
    15001 tgtgttaggt ggtgatgccc gcctcaccag cgaaacctta aaactggcgc tggcgaaagg
    15061 tttacaggat gcgggcgtcg atgtgctgga tattggtatg tccggcaccg aagaaatcta
    15121 tttcgccacg ttccatctcg gcgtggatgg cggcattgaa gttaccgcca gccataaccc
    15181 gatggattac aacggtatga aactcgtgcg cgagggggcc cgcccgatca gcggggatac
    15241 cggactgcgc gacgtccagc gtctggcaga agccaacgac tttcctcccg ttgatgaaac
    15301 caaacgcggt cgctatcagc aaatcaatct gcgtgacgct tacgttgatc acctgttcgg
    15361 ttatatcaac gtcaaaaacc tcacgccgct caagctggtg atcaactccg ggaacggcgc
    15421 agcgggtccg gtggtggacg ccattgaagc ccgctttaaa gccctcggcg caccagtgga
    15481 attgatcaaa gtgcacaaca cgccggacgg caatttcccc aacggaattc ctaacccgct
    15541 gctgccggaa tgccgcgacg acacccgcaa tgcggtcatc aaacacggcg cggatatggg
    15601 cattgccttt gatggcgatt ttgaccgctg ttttctgttt gacgaaaaag ggcagtttat
    15661 cgagggctac tacattgtcg gcctgctggc agaagcgttc ctcgaaaata atcccggcgc
    15721 gaagatcatc cacgatccgc gtctctcctg gaacaccgtt gatgtggtga ctgccgcagg
    15781 cggcaccccg gtaatgtcga aaaccggaca cgcctttatt aaagaacgga tgcgcaagga
    15841 agacgccatc tacggtggcg aaatgagcgc ccaccattac ttccgtgatt tcgcttactg
    15901 cgacagcggc atgatcccgt ggctgctggt cgccgaactg gtgtgcctga aagggaaaac
    15961 gctgggcgaa ctggtgcgtg accggatggc agcatttccg gcaagcggtg agatcaacag
    16021 caaactggcg gaccctgttg aggcgattaa ccgcgtcgaa cagcatttta gccgcgaggc
    16081 gctggcggtg gatcgcaccg atggcatcag catgaccttt gccgactggc gctttaacct
    16141 gcgcacctcc aacaccgaac cggtggtgcg cctgaatgtt gaatcgcgcg gtgatgtgcc
    16201 gctgatggaa gcgcgaacgc gaactctgct gacgttgctg aacgagtaat gtcggatctt
    16261 cccttacccc actgcgggta aggggctaat aacaggaaca acgatgacaa atctaaaaaa
    16321 gcgcgagcga gcgaaaacca atgcatcgtt aatctctatg gtgcaacgct tttcagatat
    16381 caccatcatg tttgccggac tatggctggt ttgcgaagtc agcggactgt cattcctcta
    16441 catgcacctg ttggtggcgc tgattacgct ggtggtgttc cagatgctgg gcggcatcac
    16501 cgatttttat cgctcatggc gcggtgttcg ggcagcgaca gaatttgccc tgctgctaca
    16561 aaactggacc ttaagcgtga ttttcagcgc cgggctggtg gcgttcaaca atgatttcga
    16621 cacgcaactg aaaatctggc tggcgtggta tgggctgacc agtatcggac tggtggtttg
    16681 ccgttcgtgt attcgcattg gggcgggctg gctgcgtaat catggctata acaagcgcat
    16741 ggtcgcggtg gcgggggatt tagccgccgg acaaatgctg atggagagct tccgtaatca
    16801 gccgtggtta gggtttgaag tggtgggcgt ttaccacgac ccgaaaccgg gtggcgtttc
    16861 taacgactgg gcgggcaatc tgcaacagct ggtcgaggat gcgaaagcgg gcaagattca
    16921 taacgtctat atcgcgatgc aaatgtgcga cggcgcacgc gtgaaaaaac tggtccatca
    16981 attggcggac accacctgtt cggtgctgct gatccccgat gtctttacct tcaacattct
    17041 ccattcccgt atcgaagaga tgaacggcgt accggtggtg ccgctgtatg acacgccgct
    17101 ttccggggtt aaccgcctgc tcaaacgtgc ggaagatatt gtgctggcga cgcttatcct
    17161 gctgctgatc tccccggtgc tgtgctgcat tgcgctggcg gtgaaactga gctcaccggg
    17221 gccggttatt ttccgccaga ctcgctacgg catggacggc aagccgatca aagtgtggaa
    17281 gttccgttcc atgaaagtga tggagaacga caaagtggtg acccaggcga cgcagaacga
    17341 tccgcgcgtc accaaagtgg ggaactttct gcgccgcacc tcgctggatg aattgccgca
    17401 gtttatcaat gtgctgactg gcgggatgtc gattgtcggg ccacgtccgc acgcggtggc
    17461 gcataacgaa cagtatcgcc agctcattga aggctacatg ctgcgccata aagtgaaacc
    17521 gggcattacc ggctgggcgc agattaacgg ctggcgcggc gaaaccgaca cactggagaa
    17581 aatggaaaaa cgcgtcgagt tcgaccttga gtacatccgc gaatggagcg tctggttcga
    17641 tatcaaaatc gttttcctga cggtattcaa gggcttcgtt aacaaagcgg catattgata
    17701 tgaacttacg tgaaaaaacc atcagcggcg cgaagtggtc ggcgattgcc acggtgatca
    17761 tcatcggcct cgggctggtg cagatgaccg tgctggcgcg gattatcgac aaccaccagt
    17821 tcggtttgct taccgtgtcg ctggtgatca tcgcgctggc agatacgctt tctgacttcg
    17881 gcatcgctaa ctcgattatt cagcgaaaag aaatcagcca ccttgaactc accacgctgt
    17941 actggctgaa cgtcgggctg gggatcgtgg tgtgcgtggc ggtgtttttg ttgagtgata
    18001 ccattggcga cgtgctgaat aacccggacc tggcaccgtt gattaaaaca ttgtcgctgg
    18061 cgtttgtggt gatcccccac gggcagcagt tccgcgcgtt gatgcagaag gagctggagt
    18121 tcaacaaaat cggcatgatc gaaaccagtg cggtgctggc gggcttcact tttacggtgg
    18181 ttagcgccca tttctggccg ctggcgatga ccgcgatcct cggttatctg gtcaatagtg
    18241 cggtaagaac gctgctgttt ggctactttg gccgcaaaat ttatcgcccc ggtctgcatt
    18301 tctcgctggc gtcggtggca ccaaacttac gttttggtgc ctggctgacg gcggacagca
    18361 tcatcaacta tctcaatacc aacctttcaa cactcgtgct ggcgcgtatt ctcggcgcgg
    18421 gcgtggcagg gggatacaac ctggcgtaca acgtggccgt tgtgccgccg atgaaactga
    18481 accccatcat cacgcgcgtg ttgtttccgg catttgccaa aattcaggac gacaccgaaa
    18541 agctgcgcgt caacttctac aagctgctgt cggtggtggg gattatcaac tttccggcgc
    18601 tgctggggct gatagtggtg tcgaataact ttgtaccgct ggtatttggt gagaagtgga
    18661 acagcattat tccggtgctg caattgctgt gtgtggtggg gctgctgcgc tcagtgggga
    18721 acccgattgg ttcgctgctg atggcgaaag cgcgggtcga tatcagcttt aaattcaacg
    18781 tattcaaaac ctttctgttt attccggcga ttgttattgg tgggcagatg gcgggcgcaa
    18841 tcggcgtcac gcttggcttc ctgctggtgc agattatcaa caccattctg agctatttcg
    18901 tgatgattaa accggtgctc ggttccagtt atcgccagta catcctgagt ttgtggctgc
    18961 cgttttatct ctcgctgccg acgctggtgg tcagttatgc gctgggcatt gtgctgaaag
    19021 ggcaactggc gctggggatg ctgctggcgg tgcaaatagc cacgggcgtg ctggcgtttg
    19081 tggtgatgat tgtgctgtcg cgccatccgc tggtggtgga agtgaagcgt cagttttgtc
    19141 gcagcgaaaa aatgaaaatg cttttacggg cggggtgaat ggttgttttc ggttccggca
    19201 acggttgccg gatgcggcgt aaaacgcctt atccgtcctg gggggctggg cttggatctt
    19261 gtaggcctga taagacgcgc cagcgtcgca tcaggcactg aatgccggat gcggcgtgaa
    19321 cgccttatcc ggcctacggt tctgtgctcg gatcttgtag gcctgataag acgcgtcagc
    19381 gtcgcatcag gcactgaatg ccggatgcgg cgtaaaacgc cttatccgtc ctacggctct
    19441 gtgctcgggt cttgtaggcc tgataagacg cgtcagcgtc gcatcaggca ctgaatgccg
    19501 gaagcggcgt gaacgcctta tccgtcctac ggttctgggc ttggatcttg taggcctgat
    19561 aagaggcgtc agcgtcgcat caggtactga atgccggatg cggcgtgaac gccttatccg
    19621 tcctacggtt ctgggcttgg atcttgtagg cctgataaga cgcgtcagcg tcgcatcagg
    19681 cactgaatgc cggatgcggc gtgaacgcct tatccgtcct acggttctgt gctcggatct
    19741 tgtaggcctg ataagacgcg ttagcgtcgc atcaggcaaa aaacggcaac gaacacaaca
    19801 aacacagcct cacctggcat caaatttatg aggaaaaaat gaaattactt attctgggca
    19861 accacacttg cggcaatcgt ggcgatagcg ccatcctgcg tggcttactt gatgccatca
    19921 atatcctcaa tccacatgcc gaagtggacg tgatgagccg ctatccggtc agttcttcct
    19981 ggctgctcaa ccgcccggta atgggcgatc cgctgttcct gcaaatgaaa caacacaaca
    20041 gcgcggcggg cgttgtcggg cgtgttaaaa aagtcctccg tcgccgctac cagcaccagg
    20101 tattgctctc acgcgtcacc gacactggca agctgcgtaa tatcgccatc gcccagggat
    20161 tcactgactt cgtgcgactg ctgtcaggtt acgacgccat tattcaggtc ggcggatcgt
    20221 tttttgtcga tctctacggc gtgccgcagt ttgaacatgc gctttgcact ttcatggcga
    20281 aaaagccgct gtttatgatt ggtcacagcg tcggcccctt ccaggatgag caatttaacc
    20341 aactggcgaa ctatgtcttt ggtcactgcg acgcgctgat cctgcgcgaa tcggtcagcc
    20401 ttgatttgat gaaacgcagc aatatcacca ctgcaaaagt ggaacatggc gtcgataccg
    20461 cgtggctggt cgatcaccac attgaagact tcaccgccag ctatgctgtt caacactggc
    20521 tggatgttgc cgcacaacag aaaacggtgg caattaccct gcgcgaactg gcaccgttcg
    20581 acaaacgtct cggcaccact caacaagcgt atgaaaaagc ctttgccggg gtggtcaatc
    20641 gcattctcga tgaagggtat caggtgattg cgctctccac ctgtacgggc atcgacagtt
    20701 ataacaaaga cgaccgcatg gtggcgctca atctgcgcca gcacatcagc gatcctgccc
    20761 gttatcacgt agtgatggat gaactcaacg atctggaaat gggcaaaatt ctgggggcct
    20821 gtgaacttac cgtcggtacg cgcctgcact ctgctattat ctcgatgaac tttgccactc
    20881 cggcgattgc catcaactac gaacataaat ccgccgggat catgcaacaa ctggggctac
    20941 cagaaatggc gattgatatc cgtcatttat tagacggcag cctgcaagcg atggttgcgg
    21001 ataccttagg ccagcttccg gcactgaaca cgcgacttaa cgaagccgtc agtcgcgaac
    21061 gtcagacggg aatgcagatg gtgcaatctg tgcttgaacg catcggggag gtgaaatgaa
    21121 ggtcggcttc tttttactga aatttccgtt gtcgtcggaa accttcgttc ttaaccaaat
    21181 cactgcgttt attgatatgg gatttgaggt ggagattatc gcgctgcaaa aaggcgacac
    21241 acaaaacacc cacgcggcat ggacgaaata caaccttgct gccagaaccc gctggttaca
    21301 ggacgaacct acgggcaaag tggcgaaact gcgccaccgc gccagccaga ccttatgcgg
    21361 cattcatcgt aaaaatacct ggcaggcgct caacctcaaa cgctatggcg ccgagtcgcg
    21421 gaacctgatt ttgtctgcta tttgcggtca ggtcgcaaca ccgtttcatg ccgatgtctt
    21481 tatcgctcac tttggtccgg caggggtaac cgcagcaaaa ctccgcgaac tgggtgtcat
    21541 tcgcggcaaa attgccacta tcttccacgg tattgatatc tccagtcggg aggtgctcaa
    21601 ccattacact cccgaatatc agcaactgtt ccgccgtggc gacctgatgt taccgataag
    21661 cgatctgtgg gccggaaggc tgcaaaaaat gggctgcccg agggaaaaaa tcgccgtatc
    21721 gcgtatgggc gtggacatga cgcgctttag cccgcgtccg gtgaaagcgc ctgcaacgcc
    21781 gctggaaatc atttccgtcg cacgcttaac cgagaaaaaa ggcctgcatg tggcgatcga
    21841 agcctgccgt cagttgaaag agcagggcgt ggcatttcgc tatcgcatcc tcggcattgg
    21901 cccgtgggaa cgacgcctgc gcaccctcat cgaacaatat caactggaag atgtgataga
    21961 gatgccgggc tttaaaccga gccatgaagt gaaggcgatg ctcgacgacg cggatgtctt
    22021 cctgttgcca tcggttacag gtgcggacgg cgatatggaa ggtattccgg tggcactaat
    22081 ggaagcgatg gcggtcggca ttccggtggt ttctactctg catagcggaa taccggaact
    22141 ggtggaggcc gataaatccg gctggctggt gcctgagaac gatgctcgcg cactggcgca
    22201 acgactggcg gcgtttagcc aactggacac cgacgaactg gctccggtta tcaaacgtgc
    22261 tcgcgaaaaa gtcgaacacg attttaacca gcaggtgatc aatcgagaac tcgccagctt
    22321 gctgcaggct ttatagagga gaacgtatgc catttaaaaa actctcccga cgcaccttcc
    22381 tgacggcaag ctcggcgctt gccttcctcc ataccccctt cgcccgcgca cttccagccc
    22441 gacaaagcgt taatatcaac gattacaatc cgcacgactg gatcgcctca tttaaacaag
    22501 ccttcagtga agggcaaacg gtcgtcgtgc ctgccggatt ggtttgtgac aatatcaaca
    22561 ccggcatctt cattcctcct ggaaaaacct tacacatcct tggaagcctg cgcggcaacg
    22621 gcagagggcg atttgtctta caggacggca gccaggtgac aggggaggag ggcggcagta
    22681 tgcataacat caccctggat gtgcgcggtt ctgactgcat catcaaaggg ttggcgatga
    22741 gcggttttgg cccggtaacg cagatttata tcggcggcaa aaataaacgc gtcatgcgca
    22801 acttgaccat cgataacctg accgtcagcc acgctaatta cgccatttta cgccagggat
    22861 ttcataacca gattatcggt gccaacatca ccaactgtaa gttcagcgac ttacagggcg
    22921 acgccattga atggaatgtg gcgattaacg accgcgatat tttgatatcc gaccacgtta
    22981 ttgagcgcat caactgtacc aacggcaaaa tcaactgggg catcggcata ggccttgcgg
    23041 gaagcactta cgataacaac tatccggaag accaggcagt gaaaaacttt gtcgtggcga
    23101 atatcacggg atcagattgt cgacagttga tacatgttga aaatggtaaa cattttgtta
    23161 ttcgtaatat caaagcccgc aatatcacgc cggatttcag taagaaagca ggtattgata
    23221 acgccacggt cgctatttac ggttgtgaca atttcgtgat tgataatatt gaaatgatta
    23281 atagcgccgg gatgttaatt ggctatgggg taattaaagg caaatatctc tcgataccgc
    23341 aaaatttccg agtgaataat attcaactgg ataacactca ccttgcttat aaattgcgcg
    23401 gcatccaaat ctccgccggg aatgctgtct cctttgtggc gctgactaac attgagatga
    23461 agcgtgcctc gctggagctt cacaataaac cgcaacatct ttttatgcga aatatcaaag
    23521 tgatgcagga atcctctgtt ggacccgcat tgagcatgaa cttcgacatg cgcaaagacg
    23581 ttcgcggcgt ctttatggcg aaaaaagaaa cactgctgtc tcttgcaaat gttcatgcga
    23641 tgaatgaaag agggcaaagc tccgtcgata tcgacagggt taatcaccat attgttaatg
    23701 tggaaaagat taactttaga ttgccagaac ggagggagta gatttgcgac cattcataaa
    23761 cttactggat ataatgttct aaaagattat aatcctcaca ctaactgtta aagctggggc
    23821 tgggcagcat tgactactca gggaaggtac tcatctttat cttgtgtttc ttacgtaaca
    23881 accagactaa agtgatttat gaacgataac gttttgctca taggagcttc cggattcgta
    23941 ggaacccgac tacttgaaac ggcaattgct gactttaata tcaagaacct ggacaaacag
    24001 cagagccact tttatccaga aatcacacag attggcgatg ttcgtgatca acaggcactc
    24061 gaccaggcgt tagctggttt tgacactgtt gtgctactgg cagcggaaca ccgcgatgac
    24121 gtcagcccta cttctctcta ttatgatgtc aacgttcagg gtacccgcaa tgtgctgacg
    24181 gccatggaaa aaaatggcgt taaaaatatc atctttacca gttccgttgc tgtttatggt
    24241 ttgaacaaac acaaccctga cgaaaaccat ccacacgatc ctttcaacca ctacggcaaa
    24301 agtaagtggc aggcagagga agtgctgcgt gaatggtata acaaagcacc aacagaacgt
    24361 tcattaacca tcatccgtcc taccgttatc ttcggtgaac gcaaccgcgg taacgtctat
    24421 aacttgctga aacagatcgc tggcggcaag tttatgatgg tgggcgcagg gactaactat
    24481 aagtccatgg cttatgttgg aaacattgtt gagtttatca agtacaaact gaagaatgtt
    24541 gccgcaggtt atgaggttta taattacgtt gataagccag atctgaacat gaaccagttg
    24601 gttgctgaag ttgaacaaag tctgaacaaa aagatccctt ctatgcactt gccttaccca
    24661 ctaggaatgt tgggtggata ttgctttgat atcctgagca aaattacggg caaaaaatac
    24721 gctgtcagct ctgtgcgcgt gaaaaaattc tgcgcaacaa cacagtttga cgcaacgaaa
    24781 gtgcattctt caggttttgt ggcaccgtat acgctgtcgc aaggtctgga tcgaacactg
    24841 cagtatgaat tcgttcatgc caaaaaagac gacataacgt ttgtttctga gtaatgcaga
    24901 cgagtcagag aaaaaaggac caggttggac tacgattatt cggaaagagg tactgattaa
    24961 attatcagta tgcggaattc cgagtaatcc gagtaaccca gatgctgatg gtgtgtagtg
    25021 ttattctaag gtttaagcaa agttatgcgg aactaagctt tggtatatag ccataacgtt
    25081 tggctaacct atggcaataa tttacgcact atcagccact tagaggtagg aaaagaatga
    25141 cgaatttaaa agcagttata ccggtagcag gtcttgggat gcatatgttg cctgccacta
    25201 aggcgattcc caaagagatg ctaccgatcg tcgacaagcc aatgattcag tacattgttg
    25261 acgagattgt ggctgcaggg atcaaagaaa tcctcctggt aactcacgcg tccaagaacg
    25321 cggtcgaaaa ccacttcgac acctcttatg aattagaatc tctccttgag cagcgcgtga
    25381 agcgtcaact actggcggaa gtgcaatcta tctgcccgcc gggcgtgact attatgaacg
    25441 tgcgtcaggg cgaaccttta ggtttaggtc actccatttt gtgtgcacgc cccgccattg
    25501 gtgacaaccc atttgtcgtg gtgctgccgg acgttgttat cgatgatgcc agcgccgacc
    25561 cgctgcgtta caaccttgcc gctatgattg cgcgcttcaa cgaaacgggc cgcagccagg
    25621 tgctggcaaa acgtatgccg ggtgacctct ctgaatactc cgtcatccag accaaagaac
    25681 cgctggatcg tgaaggcaaa gtcagccgca ttgttgaatt catcgaaaaa ccggatcagc
    25741 cgcagacgct ggactcagac atcatggccg ttggtcgcta tgtgctttct gccgatattt
    25801 ggccggaact ggaacgcact cagcctggtg catgggggcg tattcagctg actgatgcta
    25861 ttgccgaact ggcgaaaaaa cagtccgttg atgccatgct gatgactggt gacagctacg
    25921 actgtggtaa aaaaatgggc tatatgcagg cgtttgtgaa gtatggactg cgcaacctga
    25981 aagaaggggc gaagttccgt aaaggtattg agaagctgtt aagcgaataa tgaaaatctg
    26041 accggatgta acggttgata agaaaattat aacggcagtg aagattcgtg gcgaaagtaa
    26101 tttgttgcga atattcctgc cgttgtttta tataaacaat cagaataaca aagagttagc
    26161 aataggattt tcgtcaaagt tttccaggat tttccttgtt tccagagcgg attggtaaga
    26221 caattagcgt ttgaattttt cgagttaagc gcgagtgggt aacgctcgtc acatcgtagg
    26281 catgcatgca gtgctctggt agctgtaaag ccaggggcgg tagcgtgcat taatacctct
    26341 attaatcaaa ctaagagccg cttatttcac agcatgctct gaagtaatat ggaataaaag
    26401 agtgaagata cttgttactg gtggcgcagg atttattggt tctgctgtag ttcgtcacat
    26461 tataaataat acgcaggata gtgttgttaa tgtcgataaa ttaacgtacg ccggaaacct
    26521 ggaatcactt gctgatgttt ctgattctga acgctatatt tttgaacatg cggatatttg
    26581 cgatgctgct gcaatggcgc ggatttttgc tcagcatcag ccggatgcag tgatgcacct
    26641 ggctgctgaa agccatgtgg atcgttcaat tacaggccct gcggcattta ttgaaaccaa
    26701 tattgttggt acttatgtcc ttttggaagc cgctcgcaat tattggtctg ctcttgatgg
    26761 cgacaagaaa aatagcttcc gttttcatca tatttctact gacgaagtct atggtgattt
    26821 gcctcatcct gacgaagtaa ataataaaga acaattaccc ctctttactg agacgacagc
    26881 ttacgcgcct agtagtcctt attccgcatc aaaagcatcc agcgatcatt tagtccgtgc
    26941 gtggaaacgt acctatggtt taccgaccat tgtgactaat tgttcgaata actacggtcc
    27001 ttatcacttt ccggaaaaat tgattccact agtaattctt aatgctctgg aaggtaaggc
    27061 attacctatt tatggcaaag gggatcaaat tcgcgactgg ctgtatgttg aagatcatgc
    27121 acgtgcgtta tataccgtcg taaccgaagg taaagcgggt gaaacttata acattggtgg
    27181 acacaacgaa aagaaaaaca tcgatgtagt gctcactatt tgtgatttgt tggatgagat
    27241 tgtaccgaaa gagaaatctt accgcgagca aattacttat gttgccgatc gtccgggaca
    27301 cgatcgccgt tatgccattg atgctgagaa gattggtcgc gaattgggat ggaaaccaca
    27361 ggaaacgttt gagagcggga ttcggaagac agtggaatgg tacctgtcca atacaaaatg
    27421 ggttgataat gtgaaaagtg gtgcctatca atcgtggatt gaacagaact atgagggccg
    27481 ccagtaatga atattctcct tttcggcaaa acagggcagg taggttggga actacagcgt
    27541 gctctggcac ctttgggtaa tttgattgct cttgatgttc actccactga ttattgtggt
    27601 gattttagta atcctgaagg tgtggctgaa accgtcaaaa aaattcgccc tgatgttatt
    27661 gttaatgctg cggctcatac cgcagtagat aaggctgagt cagaacccga atttgcacaa
    27721 ttactcaatg cgaccagcgt tgaatcaatt gcaaaagcgg ctaatgaagt tggggcctgg
    27781 gtaattcatt actcaactga ctacgtattt ccgggaaccg gtgaaatacc atggctggag
    27841 acggatgcaa ccgcaccgct aaatgtttac ggtgaaacca agttagctgg agaaaaagca
    27901 ttacaagagc agtgtgcgaa gcaccttatt ttccgtacca gctgggtata tgcaggtaaa
    27961 ggaaacaatt ttgccaagac aatgttacgt ctggcaaaag agcgtgaaga attagccgtt
    28021 attaatgatc agtttggtgc gccaactggc gcagagttgc tggctgattg tacggcacat
    28081 gccattcgtg tggcactgaa taaaccggaa gtcgcaggtt tgtaccatct ggtagccagt
    28141 ggtaccacaa cctggcacga ttatgctgcg ctggtttttg aagaggcgcg caaagcaggc
    28201 ataccccttg cactcaacaa gctcaacgca gtagcaacaa cagcctatcc tacaccagct
    28261 cgtcgtccac ataactctcg ccttaataca gaaaaatttc agcagaattt tgcgcttgtt
    28321 ttgcctgact ggcaggttgg cgtgaaacga atgctcaacg aattatttac gactacagta
    28381 atttaatagt ttttgcatct tgttcgtgat gatggagcaa gatgaattaa aaggaatgat
    28441 gaaatgaaaa cgcgtaaagg tattatttta gcgggtggct ctggtactcg tctttatcct
    28501 gtgactatgg ctgtcagtaa acagctgtta cctatttatg ataaaccgat gatctattac
    28561 ccgctttcta cactgatgtt agcgggtatt cgcgatattc tgattatcag tacaccacag
    28621 gatactcctc gttttcacca actgctgggt gacgggggcc agtgggggct aaatcttcag
    28681 tacaaagtgc aaccgactcc agatgggctt gcgcaggcgt ttattatcgg tgaagagttt
    28741 atcggtggtg atgattgtgc tctggttctc ggtgataata tcttttacgg tcatgatctg
    28801 ccgaagttaa tggatgtcgc tgttaacaaa gaaagtggtg caacagtatt tgcctatcat
    28861 gttaatgatc ctgaacgcta cggtgtcgtt gagtttgata aaaaaggtac ggcaattagc
    28921 ctggaagaaa aaccgttaca accaaaaagt aattatgcgg taaccgggct ttatttctat
    28981 gataacgacg ttgtcgaaat ggcgaaaaac cttaagcctt ctgcccgtgg tgaactggaa
    29041 attaccgata ttaaccgtat ttatatggaa cagggggggt tatccgttgc catgatggga
    29101 cgtggttatg catggctgga cacgggtaca catcaaagtc ttattgaagc aagcaacttc
    29161 attgcaacaa ttgaagagcg tcaggggtta aaagtttcct gtccggaaga aattgcttac
    29221 cgtaaagggt ttattgatgc tgagcaggtg aaagtattag ctgaaccgct gaaaaaaaat
    29281 gcttatggtc agtatctgct gagaatgatt aaaggttatt aataaaatga acgtaatt
//
LOCUS       DAZGHF010000053        28900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGHF010000053 DAZGHF010000000
VERSION     DAZGHF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.053"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            92..1498
                     /gene="fliD"
                     /locus_tag="ACHK3A_002412"
     CDS             92..1498
                     /gene="fliD"
                     /locus_tag="ACHK3A_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO8740478.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLIIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            1523..1933
                     /gene="fliS"
                     /locus_tag="ACHK3A_002413"
     CDS             1523..1933
                     /gene="fliS"
                     /locus_tag="ACHK3A_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO8740479.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPD"
     gene            1933..2298
                     /gene="fliT"
                     /locus_tag="ACHK3A_002414"
     CDS             1933..2298
                     /gene="fliT"
                     /locus_tag="ACHK3A_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO8740480.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            2376..3863
                     /gene="amyA"
                     /locus_tag="ACHK3A_002415"
     CDS             2376..3863
                     /gene="amyA"
                     /locus_tag="ACHK3A_002415"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO8740481.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(3897..4310)
                     /gene="yedD"
                     /locus_tag="ACHK3A_002416"
     CDS             complement(3897..4310)
                     /gene="yedD"
                     /locus_tag="ACHK3A_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO8740482.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     SALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            4497..5702
                     /gene="yedE"
                     /locus_tag="ACHK3A_002417"
     CDS             4497..5702
                     /gene="yedE"
                     /locus_tag="ACHK3A_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO8740483.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            5699..5932
                     /gene="yedF"
                     /locus_tag="ACHK3A_002418"
     CDS             5699..5932
                     /gene="yedF"
                     /locus_tag="ACHK3A_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO8740484.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            6041..6709
                     /gene="yedK"
                     /locus_tag="ACHK3A_002419"
     CDS             6041..6709
                     /gene="yedK"
                     /locus_tag="ACHK3A_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO8740485.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAVREWMRQEVGGKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAAL
                     IQPV"
     gene            6820..7299
                     /gene="yedL"
                     /locus_tag="ACHK3A_002420"
     CDS             6820..7299
                     /gene="yedL"
                     /locus_tag="ACHK3A_002420"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8740486.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PKVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELP"
     gene            7539..7679
                     /locus_tag="ACHK3A_002421"
                     /pseudo
     CDS             7539..7679
                     /locus_tag="ACHK3A_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(7704..8018)
                     /gene="fliE"
                     /locus_tag="ACHK3A_002422"
     CDS             complement(7704..8018)
                     /gene="fliE"
                     /locus_tag="ACHK3A_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO8740487.1"
                     /translation="MSAIQGIEGVISQLQATAMSARTQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            8233..9891
                     /gene="fliF"
                     /locus_tag="ACHK3A_002423"
     CDS             8233..9891
                     /gene="fliF"
                     /locus_tag="ACHK3A_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO8740488.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            9884..10879
                     /gene="fliG"
                     /locus_tag="ACHK3A_002424"
     CDS             9884..10879
                     /gene="fliG"
                     /locus_tag="ACHK3A_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO8740489.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            10872..11558
                     /gene="fliH"
                     /locus_tag="ACHK3A_002425"
     CDS             10872..11558
                     /gene="fliH"
                     /locus_tag="ACHK3A_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO8740490.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGVAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            11558..12931
                     /gene="fliI"
                     /locus_tag="ACHK3A_002426"
     CDS             11558..12931
                     /gene="fliI"
                     /locus_tag="ACHK3A_002426"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO8740491.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGSALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            12950..13393
                     /gene="fliJ"
                     /locus_tag="ACHK3A_002427"
     CDS             12950..13393
                     /gene="fliJ"
                     /locus_tag="ACHK3A_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO8740492.1"
                     /translation="MTEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            13390..14517
                     /gene="fliK"
                     /locus_tag="ACHK3A_002428"
     CDS             13390..14517
                     /gene="fliK"
                     /locus_tag="ACHK3A_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO8740493.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETPPVINDKQSTSTSLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSSVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            14622..15086
                     /gene="fliL"
                     /locus_tag="ACHK3A_002429"
     CDS             14622..15086
                     /gene="fliL"
                     /locus_tag="ACHK3A_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO8740494.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            15091..16095
                     /gene="fliM"
                     /locus_tag="ACHK3A_002430"
     CDS             15091..16095
                     /gene="fliM"
                     /locus_tag="ACHK3A_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO8740495.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            16092..16505
                     /gene="fliN"
                     /locus_tag="ACHK3A_002431"
     CDS             16092..16505
                     /gene="fliN"
                     /locus_tag="ACHK3A_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO8740496.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            16508..16873
                     /gene="fliO"
                     /locus_tag="ACHK3A_002432"
     CDS             16508..16873
                     /gene="fliO"
                     /locus_tag="ACHK3A_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO8740497.1"
                     /translation="MNNHATVQSSAPVSTAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            16873..17610
                     /gene="fliP"
                     /locus_tag="ACHK3A_002433"
     CDS             16873..17610
                     /gene="fliP"
                     /locus_tag="ACHK3A_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO8740498.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLTGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            17620..17889
                     /gene="fliQ"
                     /locus_tag="ACHK3A_002434"
     CDS             17620..17889
                     /gene="fliQ"
                     /locus_tag="ACHK3A_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO8740499.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            17898..18683
                     /gene="fliR"
                     /locus_tag="ACHK3A_002435"
     CDS             17898..18683
                     /gene="fliR"
                     /locus_tag="ACHK3A_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO8740500.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            18973..19596
                     /gene="rcsA"
                     /locus_tag="ACHK3A_002436"
     CDS             18973..19596
                     /gene="rcsA"
                     /locus_tag="ACHK3A_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO8740501.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(19640..19828)
                     /gene="dsrB"
                     /locus_tag="ACHK3A_002437"
     CDS             complement(19640..19828)
                     /gene="dsrB"
                     /locus_tag="ACHK3A_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO8740502.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            19991..20218
                     /gene="yodD"
                     /locus_tag="ACHK3A_002438"
     CDS             19991..20218
                     /gene="yodD"
                     /locus_tag="ACHK3A_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO8740503.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            20517..21332
                     /gene="yedP"
                     /locus_tag="ACHK3A_002439"
     CDS             20517..21332
                     /gene="yedP"
                     /locus_tag="ACHK3A_002439"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO8740504.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAKQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(21329..23023)
                     /gene="dgcQ"
                     /locus_tag="ACHK3A_002440"
     CDS             complement(21329..23023)
                     /gene="dgcQ"
                     /locus_tag="ACHK3A_002440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO8740505.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     VGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(23194..23376)
                     /gene="yodC"
                     /locus_tag="ACHK3A_002441"
     CDS             complement(23194..23376)
                     /gene="yodC"
                     /locus_tag="ACHK3A_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO8740506.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(23455..24372)
                     /gene="yedI"
                     /locus_tag="ACHK3A_002442"
     CDS             complement(23455..24372)
                     /gene="yedI"
                     /locus_tag="ACHK3A_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO8740507.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            24545..25465
                     /gene="yedA"
                     /locus_tag="ACHK3A_002443"
     CDS             24545..25465
                     /gene="yedA"
                     /locus_tag="ACHK3A_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO8740508.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(25454..25924)
                     /gene="vsr"
                     /locus_tag="ACHK3A_002444"
     CDS             complement(25454..25924)
                     /gene="vsr"
                     /locus_tag="ACHK3A_002444"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO8740509.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(25905..27323)
                     /gene="dcm"
                     /locus_tag="ACHK3A_002445"
     CDS             complement(25905..27323)
                     /gene="dcm"
                     /locus_tag="ACHK3A_002445"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO8740510.1"
                     /translation="MQENISVTDSYSTGNVVQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEVKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(27390..28085)
                     /gene="yedJ"
                     /locus_tag="ACHK3A_002446"
     CDS             complement(27390..28085)
                     /gene="yedJ"
                     /locus_tag="ACHK3A_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO8740511.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQHSSILAAEEARRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(28125..28406)
                     /gene="drpB"
                     /locus_tag="ACHK3A_002447"
     CDS             complement(28125..28406)
                     /gene="drpB"
                     /locus_tag="ACHK3A_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO8740512.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         7070 a         7550 c         7394 g         6886 t
ORIGIN      
        1 attaaaaaaa acaattaaac gtaaactttg cgcaattcag gccgataacc ccggtattcg
       61 ttttacgtgt cgaaagataa aaggaaatcg catggcaagt atttcatcgc tgggagtcgg
      121 gtcaggtctg gatttaagtt caatccttga tagcctcacc gccgcgcaaa aagcgacgct
      181 aacccctatt tcaaatcagc aatcgtcgtt taccgctaaa cttagcgcct acggtacgct
      241 gaaaagcgcg ctgacgactt tccagaccgc caatactgca ttgtctaaag ccgatctttt
      301 ttccgccacc agcactacca gcagcaccac cgcgttcagt gccaccactg cgggtaacgc
      361 catcgccggg aaatacacca tcagcgtcac ccatctggcg caggcgcaaa ccctgaccac
      421 gcgcaccacc agagacgata cgaaaacggc gatcgccacc agcgacagta aactcatcat
      481 tcaacaaggc ggcgacaaag atccgattac cattgatatc agcgcggcta actcatcgtt
      541 aagcgggatc cgtgatgcta tcaacaacgc aaaagcgggc gtaagcgcaa gcatcattaa
      601 cgtgggtaac ggtgaatatc gtctgtcagt cacatcaaat gacaccggcc ttgataatgc
      661 gatgacactc tcggtcagcg gtgatgatgc gctacaaagt tttatgggct atgacgccag
      721 tgccagcagc aacggcatgg aggtctcggt cgccgcccag aatgcgcagc tgacggtcaa
      781 caacgtcgcc attgagaaca gcagcaacac catcagcgac gcgctggaaa acatcaccct
      841 gaacctgaac gatgtcacca cgggcaacca gacgctaacc atcactcagg acacctccaa
      901 agcgcaaacg gcgattaaag actgggtgaa tgcctataac tcgctaatag ataccttcag
      961 cagcctgacc aaatacaccg ccgtagatgc gggagctgat agccagagtt ctagcaatgg
     1021 cgcactgctc ggcgactcca cgttgcggac gattcagacg cagttgaaat cgatgctgag
     1081 taataccgtt agttcttcca actataaaac gttggcgcag attggtatca cgaccgatcc
     1141 cagcgatggc aaactggaac tggatgccga caaactcacc gctgcactga aaaaagatgc
     1201 cagcggcgta ggtgcattga ttgttggcga tggtaaaaaa accggcatca cgaccaccat
     1261 cggcagcaac ctgaccagtt ggctttcgac aacaggcatt attaaagccg ctaccgatgg
     1321 cgttagtaag accctgaata agttaaccaa agactacaac gccgccagcg atcgcattga
     1381 tgcgcaggtc gcgcgctaca aagagcaatt tacccaactg gacgttttaa tgacctcgtt
     1441 aaacagcacc agcagctact taacgcagca gttcgaaaac aacagtaatt ccaagtaatc
     1501 aatattcatc gggagacagg tcatgtacgc ggcaaaaggc acccaggcct atgcacaaat
     1561 tggcgtcgaa agcgccgtaa tgagcgccag ccagcaacag ctggtcacca tgctatttga
     1621 tggagtgctg agcgcactgg ttagagcgcg cctgtttatg caggacaaca atcagcaagg
     1681 caaaggcgtc tctttgtcaa aagcgatcaa catcattgag aacgggctgc gggtgagtct
     1741 tgatgaagag agcaaagacg aactaaccca aaacttgatt gctctttata gctatatggt
     1801 caggcgcttg ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag aagtggaagc
     1861 attaatgcgc aatattgccg atgcctggaa agagtcttta ctctcccctt ctttgattca
     1921 ggacccagac tgatgaacaa tgcaccgcat ttgtatttcg cctggcaaca actcgtcgaa
     1981 aaaagtcagc tcatgttacg cctggcgaca gaagaacaat gggacgaact catcgccagc
     2041 gaaatggcgt atgtgaatgc ggtgcaggag atcgcacacc tgactgaaga ggttgccccg
     2101 tccaccacga tgcaggagca gctccgcccg atgctgcgcc tgattctcga caacgaaagc
     2161 aaggtaaagc agttattaca gattcggatg gatgaactgg cgaaactggt cggtcagtca
     2221 tcggtgcaaa aatcggtgtt aagtgcctat ggcgatcagg gtggctttgt gctggctccg
     2281 caggataacc tcttttgaat cttaaagagc caatggctcg cgaataatct gattacggct
     2341 acgcttctaa tgttcccctt gaatggagtc gaagaatgcg taatcccacg ctgttacaat
     2401 gttttcactg gtattacccg gaaggcggta agctctggcc tgaactggcc gagcgcgccg
     2461 acggttttaa tgatattggt atcaatatgg tctggttgcc gcccgcctat aaaggcgcat
     2521 cgggcgggta ctcggtcggc tacgactcct atgatttatt tgatttaggc gagtttgatc
     2581 agaaaggcag catccctact aaatatggcg ataaagcaca actgctggcc gccattgacg
     2641 ccctgaaacg taatgacatt gcggtgctgc tggacgtggt agtcaaccac aaaatgggcg
     2701 cggatgaaaa agaagctatt cgcgtgcaac gtgtaaatgc tgatgaccgt acgcaaattg
     2761 acgaagaaat cattgagtgc gaaggctgga cgcgttacac cttccccgcg cgtgccgggc
     2821 aatactcgca gtttatctgg gatttcaaat gttttagcgg tatcgaccat atcgaaaacc
     2881 ctgatgaaga tggcattttt aaaattgtta acgactatac cggagaaggc tggaacgatc
     2941 aggttgatga tgaactcggt aatttcgatt atctgatggg tgagaatatc gattttcgca
     3001 atcatgccgt gacggaagag attaaatact gggcgcgctg ggtgatggaa caaacgcaat
     3061 gcgacggctt tcgtcttgat gcggtcaaac atattccggc atggttttat aaagagtgga
     3121 tcgaacacgt acaggaagtt gcgccaaagc cgctgtttat tgtggctgaa tactggtcgc
     3181 atgaagtcga taaactgcaa acgtatattg atcaggtgga aggcaaaacc atgctgtttg
     3241 atgcgccgct gcagatgaaa ttccatgaag catcgcgcat gggtcgcgac tacgacatga
     3301 cgcagatctt cactgggacg ctggtggaag ccgatccttt tcacgccgta accctcgttg
     3361 ccaaccacga cacccaaccg ttgcaagccc tcgaagcacc ggtcgaaccg tggtttaaac
     3421 cgctggcgta tgccttaatt ttgttgcggg aaaatggcgt tccttcggtg ttctatccgg
     3481 acctctacgg tgcacattac gaagatgtcg gtggtgacgg gcaaacctat ccgatagata
     3541 tgccaataat cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg
     3601 tacagacgtt atttttcgac catccgaact gcattgcctt tagccgcagt ggcaccgacg
     3661 aatatcccgg ctgcgtggtg gtcatgtcga acggggatga tggcgaaaaa accattcatc
     3721 tgggagagaa ttacggcaat aaaacctggc gtgattttct ggggaatcgg caagagagcg
     3781 tagtgaccga cgaaaacggc gaagcgacct tcttttgcaa cggcggcagc gtcagcgtgt
     3841 gggttatcga agaggtgatt taaattcatc cccggcggca agccggggag atttcattac
     3901 ggcagtggcg tcggtaacgg cgctttatcc agcgcggcgg cacactctgc cgtcgggcga
     3961 tccacacgct ccatcgtcat accatcgtac tcaatggtgt taccgtcacg ctcaacctcg
     4021 tacagctcac gtttcacggt aacgttagtg agatcatcag acatcaatgt caacttaccc
     4081 ggcacggcaa tcacccgctg ccactgacgg caatccaggg tatcgccctc tttagtgaca
     4141 atcaggctgc ctatcgcttc cggactgacc agcgcacttt gcggcccttt ggtctgccag
     4201 tagcccgcca gccagtccgg cgcgggggtt ttcacgacat tgttatagtt ttcgacttcg
     4261 gcacagcccg ccagcaacat gagcgcgcct gcaattgcga gttttttcat cattcgtcct
     4321 gcatgagaag aaaagaggat tgtggcatta aagccctgat gtcgccagcc tttccaggtt
     4381 cagagagaaa tttgatctaa ctattatttt tgtgaattat taccccgaaa ataaatttgc
     4441 ttgatgtatg attcgcaact cttgtttcaa accttctgag ttcagaggct acatacatgt
     4501 catggcagca attcaaacac gcctggttga ttaaattctg ggcccccatc cctgcggtca
     4561 tcgcggcggg tattctctcc acttactact ttggcatcac tggcaccttt tgggctgtca
     4621 cgggtgaatt tacccgctgg ggcggtcagc ttctgcagct gttcggcgtc catgctgaag
     4681 agtggggtta ttttaaaatt atccatctgg aaggatcgcc attaacccgc atcgacggca
     4741 tgatgatcct cggtatgttt ggcggctgct ttgctgcagc gctgtgggcc aacaatgtca
     4801 aactacgcat gccacgcagc cgtatccgca ttatgcaggc cattattggc ggcattatcg
     4861 ctggttttgg cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt accggcattc
     4921 ctcagttctc gctgcatgcc tggttctttg ccatcgccac tgccattggt tcatggtttg
     4981 gcgcgcgctt taccctgctg cccatcttcc gtattcccgt gaaaatgcag aaagtttctg
     5041 ccgcctcacc gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc
     5101 tggtcttttt cggcatgctg ggctgggcgc tgctcacggc gatgaaccaa ccaaaactgg
     5161 ggctggcaat gctgtttggc gtcggctttg gtttactgat tgaacgtgcg caaatctgct
     5221 ttacttcggc gttccgcgat atgtggatca ccggacgtac ccatatggcg aaagcaatca
     5281 ttatcggtat ggcggtgagt gccatcggga tcttcagtta cgtacagtta ggcgttgaac
     5341 ctaaaatcat gtgggcggga ccaaatgcgg taattggtgg tttacttttt ggttttggca
     5401 tcgtgctggc aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc
     5461 actactggtg ggtcggtctg ggcaacgtga tcggctcaac gattctggcg tattactggg
     5521 atgatttcgc tccggcgctg gccaccgact gggacaaaat caacctgctg aaaacctttg
     5581 gtccgatggg tggcctgctg gtgacatatt tgctgttgtt tgcagctcta atgttgatta
     5641 tcggctggga aaaacgcttc ttccgccgtg cggcaccgca gattgctaag gagatcgcat
     5701 gaaaaatatc gttcctgatt accgtcttga tatggtgggt gaaccctgcc cttatccggc
     5761 tgtcgcaacc cttgaggcga tgccgcagtt aaaaaaaggg gagatcctgg aagtggtgag
     5821 cgactgtccg cagtcgatca ataatattcc actggatgcg cgtaatcacg gctatacggt
     5881 gctggatatt cagcaagacg ggccgaccat tcgttattta attcagaagt aatccctcat
     5941 tcccgctgtg gcccacagcg ggaacatcgt taccactcta tcacatagat tttacccgcc
     6001 ccctctccta ttcttttcag tgtgctctca ggaggtttct atgtgtggac gctttgccca
     6061 atcccaaacg cgtgaagatt acctcgcgtt tctcgcggaa gatattgaac gcgatattcc
     6121 ctacgatccc gaacctattg gcagatacaa cgtcgcgccg ggaaccaaag tccttctgct
     6181 cagtgaacgt gatgaacacc ttcatctgga tccggttttc tggggatatg ctcccggatg
     6241 gtgggataaa ccgccgctga ttaacgcccg cgtagaaact gccgcaacca gtcgcatgtt
     6301 taaaccgctc tggcaacacg gtcgggcaat ctgttttgcc gatggctggt ttgagtggaa
     6361 aaaagaaggc gacaaaaaac agccttattt tatctatcgc gctgacggac aacctgtttt
     6421 tatggccgcg ataggtagca caccgtttga gcgtggtgac gaagccgaag gatttttgat
     6481 cgtcactgcg gcggcagatc aaggtctggt agatattcat gaccgccgcc cgctggttct
     6541 gtcgccagaa gctgtacggg aatggatgcg gcaagaggtt ggcggtaaag aagcctcaga
     6601 aattgctgcc agtggctgtg tgccagcaaa ccagttcacc tggcacccgg tatcgtgcgc
     6661 ggtgggtaat gttaaaaacc agggagcggc gttaattcaa cctgtttgat aagcctggag
     6721 attattgatc aacaatactg cgtcataaga aatctctatt agacaaagat ttcagtacct
     6781 gttggcatat tgcaaaaata acaccaatac ggaatcgtca tgttcacgat taaaacagat
     6841 gatctcaccc atccagcagt gcaagcatta gtggcttacc atatttccgg catgctgcag
     6901 cagtctcccc ccgaaagtag tcatgcttta gatgtgcaaa aattacgtaa cccgaaagtg
     6961 acattctggt cagtatggga aggcgaacaa ctcgcaggaa ttggtgcgct gaagttgctg
     7021 gatgataaac atggcgaact gaaatcaatg cgtaccgcgc caaattattt acgtcggggt
     7081 gtcgccagtc tgattttacg ccacattttg caggtcgccc atgacagatg ccttcatcgc
     7141 ctgagcttag aaacgggtac acaggctgga tttacggcct gccatcaact ttatttgaaa
     7201 catggattcg ttgattgcga accgtttgct gattatcaac ttgatccgca cagtcgattt
     7261 ttgtcattga cgctatgcga aaataatgaa ttgccatgag ccagacgcag cacattcttg
     7321 cattcgacgt gctgcgcctt tatttattat cagccgcaaa tgtcttgtcc ttagacgctc
     7381 tttccacatg cgccacaaga aacctctatt ccagtgacac aattatgcct gattatttcc
     7441 ctagcccgga atatgaataa aaacatcaaa taagatgaaa ttatcaaaat attaatgttg
     7501 cgtctgaggt cgtaacaaca tccgacgtgg tgatatgtca aatgagacac tctgatggtc
     7561 aaagggttta tcgcgtttac agatcgtgga tggcagaaaa ctttcagaat caggtttcca
     7621 ttttaaacca gaaattaagt gagtttgtcc catccatgcc ccacgcggtg agatcggatg
     7681 taatcaacaa caggttacaa aacctacacc tgcatgctca tcacttcctg atacgccgcc
     7741 accagcttat tacgcacctg aatccccatt tgcattgaaa ctgaggcttt ttgcatatcg
     7801 gtcatcacat cgtttaacgc cacgccgggt tcaccgagag tgaatttttc tgcctgcgtg
     7861 cgggcggctg tttgtgtatc gcttatgcga tcgagcgcgg cgtgcagctg cccggcaaaa
     7921 ctaatggtcg gttgtggcag tgattcctgt gtacgcgcac tcatcgccgt agcctgtaac
     7981 tggctgataa ccccttcaat cccctgtatc gctgacattt tcatctcccg gataatttct
     8041 ggcagcaaag cctaccagta agtcaataag acaaaggcgc taaatagcaa caaaaaaacg
     8101 ggtttattgg cggatagaaa aaaacgaaag cacaaataat gggagcgtca atttttcgag
     8161 tttgctgacc cgggagtgag tcttgttcca ctttgccaat aacgccgtcc ataatcagcc
     8221 acgaggtgcg cgatgaatgc gactgcagcc cagacaaaat ctcttgagtg gcttaatcgc
     8281 ctgcgtgcga atccgaaaat tccattgatt gttgccggct ccgcagcagt ggcggtcatg
     8341 gtcgcactga tcctgtgggc gaaagccccc gactaccgca cattattcag caatctttcc
     8401 gatcaggatg gtggcgcaat tgtcagccaa ctgacgcaaa tgaatattcc ttaccgcttc
     8461 agcgaagcca gcggcgctat tgaagttccg gcagataaag ttcacgaact gcgcctgcgc
     8521 ctggcgcaac aaggtttgcc caaaggcggc gcggtcggtt tcgaactgct cgatcaggaa
     8581 aagtttggta tcagccagtt cagcgaacag gtgaattatc agcgggcgct ggaaggcgag
     8641 ctttctcgta ccatcgaaac tatcggcccg gtaaaagggg cgcgcgtaca tctggcaatg
     8701 ccgaaaccgt ctttattcgt ccgtgaacaa aaatcccctt ctgcatcggt gacggtaaat
     8761 ctgttacccg gccgcgcact cgatgaaggg caaattagcg ccattgtaca tctggtttcc
     8821 agcgcggttg ctggtctgcc gccggggaac gtcacgctgg tggatcaggg tggacatctg
     8881 ttaacccagt ccaataccag cgggcgcgat cttaatgacg ctcagttgaa atatgccagc
     8941 gatgtcgaag gccggattca gcggcgtatt gaagcgatcc tgtcgccaat tgttggtaac
     9001 ggtaatattc acgcccaggt cacggcgcaa ctggacttcg ccagtaaaga acaaacagaa
     9061 gaacagtatc gccctaacgg tgatgaatct caggcggcgc ttcgttcacg ccagcttaat
     9121 gagagcgagc aaagcggttc cggttatcca ggcggcgtac cgggggcgtt gtcgaatcaa
     9181 ccggcacctg cgaataacgc gccaatcagc acgcctccgg caaatcaaaa taaccgccag
     9241 cagcaggcga gcaccaccag caatagtggg ccgcgtagca cacagcgtaa tgaaaccagt
     9301 aactacgaag tcgatcgcac cattcgtcat accaaaatga acgtgggcga tgtgcaacgt
     9361 ctgtcagtcg cggtagtggt gaattacaaa accttgccag acggtaaacc gttgcctctc
     9421 agcaacgaac agatgaagca aattgaagat ctgacccgcg aggcgatggg cttttctgaa
     9481 aaacgcggtg actcgctcaa tgtcgttaac tcgccgttca atagtagtga ggaaagcggc
     9541 ggagaactgc cgttctggca acaacaagcg tttatcgatc agttacttgc tgccggtcgc
     9601 tggttgctgg tactgctggt ggcgtggctg ctgtggcgta aagcggtacg cccgcagcta
     9661 acacgtcgcg ctgaggcgat gaaagctgta cagcaacagg cgcaggcccg cgaggaagtg
     9721 gaagatgcgg tggaagtccg cctgagcaaa gacgaacaac tccaacaacg gcgcgctaac
     9781 caacgtctgg gggcagaagt catgagccag cgtatccgtg aaatgtctga taacgatccg
     9841 cgcgtggtgg cgctggtcat tcgccagtgg ataaataacg atcatgagta acctgacagg
     9901 caccgataaa agcgtcatcc tgctgatgac cattggcgaa gaccgggcgg cagaggtgtt
     9961 caagcacctc tcccagcgcg aagtgcaaac tctgagcgct gcaatggcga acgtcacgca
    10021 gatctccaac aagcagctaa ccgatgtgct ggcggagttt gagcaagaag ctgaacagtt
    10081 tgccgcactg aatatcaacg ccaacgatta tctgcgttcg gtattggtca aagctctggg
    10141 tgaagaacgt gccgccagcc tgctggagga tattctcgaa acccgcgata ccgccagcgg
    10201 tattgaaacg ctcaacttta tggagccgca gagcgccgcc gatctgattc gcgatgagca
    10261 tccgcaaatt atcgccacca ttctggtgca tctgaagcgc gcccaggccg ccgatattct
    10321 ggcgttgttc gatgaacgtc tgcgccacga cgtgatgttg cgtatcgcca cctttggcgg
    10381 cgtgcagcca gccgcgctgg cggaactgac cgaagtactg aatggcttgc tcgacggtca
    10441 gaatctcaag cgcagcaaaa tgggcggcgt gagaacggca gccgaaatta tcaacctgat
    10501 gaaaacgcag caggaagaag ccgttattac cgccgtgcgt gaattcgacg gcgagctggc
    10561 gcagaaaatc atcgatgaga tgttcctgtt cgagaatctg gtggatgtcg atgatcgcag
    10621 cattcagcgt ctgttgcagg aagtggattc cgaatcgctg ctgatcgcgc tgaaaggggc
    10681 cgagcagcca ctgcgcgaga aattcctgcg caatatgtcg cagcgtgccg ccgatattct
    10741 gcgcgacgat ctcgccaacc gtggtccggt gcgtctgtcg caggtggaaa acgaacagaa
    10801 agcgattctg ctgattgtgc gccgccttgc cgaaaccggc gagatggtga ttggcagcgg
    10861 cgaggatacc tatgtctgat aatctgccgt ggaaaacctg gacgccggac gatctcgcgc
    10921 caccacaggc agagtttgtg ccaatggtcg agtcggaaga aaccattatt gaagaggccg
    10981 aacccagcct tgagcagcaa ctggcgcaac tgcaaatgca ggcccatgag caaggttatc
    11041 aggcgggtgt tgccgaaggt cgccagcaag gtcataagca gggctatcag gaaggactgg
    11101 cccaggggct ggaacaaggt ctggcagagg cgaagtctca acaagcgcca attcatgccc
    11161 ggatgcagca actggtcagc gaatttcaaa ctacccttga tgcgcttgat agtgtgattg
    11221 cttcgcgcct gatgcagatg gcgctggagg cggcacgtca ggtgatcggt cagacgccaa
    11281 cggtggataa ctcggcactg atcaaacaga tccaacagtt gttgcagcaa gaaccgttat
    11341 tcagcggtaa accacagctg cgcgtgcacc cggatgatct gcaacgtgtt gatgatatgc
    11401 tcggcgcaac tttaagtttg catggctggc gcttgcgggg cgatcccacc ctccatcctg
    11461 gcggctgtaa agtctccgcc gatgaaggcg atctcgacgc cagtgtcgcc actcgctggc
    11521 aagaactctg ccgtctggca gcaccaggag tggtgtaatg accacgcgcc tgactcgctg
    11581 gctaaccacg ctggataact ttgaagccaa aatggcgcag ttgcctgcgg tacgtcgcta
    11641 cgggcgatta acccgcgcta ccgggctggt gctggaagcc accggattac aattgccgct
    11701 cggcgcaacc tgtgtcattg agcgccagaa cggcagcgaa acgcacgaag tagaaagcga
    11761 agtcgttggc tttaacggtc aacggctgtt tttaatgccg ctggaggaag tcgaaggtgt
    11821 cctgcccggc gcgcgtgttt atgccaaaaa catttcggct gaagggctgc aaagcggcaa
    11881 gcagttgccg ctcggttcgg cgttattagg tcgcgttctg gacggcagcg gtaaaccgct
    11941 tgatggcctc ccctcccccg atacgacgga aaccggtgcg ctgattaccc cgccatttaa
    12001 cccgttgcaa cgtacaccga ttgaacatgt gctggacacc ggcgtgcgcc caatcaatgc
    12061 cctgcttacc gtcgggcgcg ggcagcgtat ggggctgttt gccgggtccg gcgttggtaa
    12121 aagtgtgctg ctggggatga tggcacgtta cacccgcgcc gatgtcattg tcgtgggttt
    12181 gattggtgaa cgagggcgcg aagtaaaaga ttttattgag aatatcctcg gtgccgaagg
    12241 acgtgcacgc tccgtggtga ttgccgctcc ggcggatgtt tctccgctcc tgcgaatgca
    12301 gggtgccgcc tatgccacgc gcattgccga agattttcgc gatcgtggtc agcatgtgtt
    12361 gctgattatg gactccctca cccgctacgc gatggcccag cgtgaaattg cgctggcgat
    12421 tggcgaacca cctgccacta aaggttatcc accgtcggtg tttgccaaat taccggcact
    12481 ggtcgagcgt gccggaaatg gcattagcgg cggcggctcg attaccgcgt tttataccgt
    12541 gctcaccgaa ggcgatgacc agcaggaccc cattgccgac tccgcgcggg caatcctcga
    12601 cggtcacatt gtgctgtctc gccgactggc ggaagccggg cactatccgg ctatcgatat
    12661 tgaagcgtcg atcagtcgcg caatgacggc gttgatcagt gagcaacatt acgcgcgagt
    12721 gcgcaccttc aaacagctgt tgtcgagttt tcagcgtaac cgcgatctgg ttagcgtcgg
    12781 cgcgtatgcc aaaggcagcg atccgatgct cgacaaagcc atcgccctgt ggccgcagct
    12841 ggagggctat ttgcaacaag gcatttttga acgcgcggac tgggaagcgt ctctccaggg
    12901 gctggagcgt attttcccga cagtatcata acccaggaga taacggcaga tgacagaaca
    12961 tggcgcgctg gcgaccctga aagatctggc agaaaaagag gtagaggatg ccgcgcgcct
    13021 gctgggtgaa atgcgtcggg gatgtcagca ggcggaagaa cagctcaaaa tgctgatcga
    13081 ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga tgaccagcaa
    13141 ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta ctcagcatcg
    13201 ccagcaactt aatcagtgga cgcagaaagt agacattgcc ctgaacagct ggcgagaaaa
    13261 gaaacaacgt ttgcaggctt ggcagacact gcaggaacgg caatccacgg cggcactgct
    13321 tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg ccgccatgag
    13381 gaaacctgaa tgattcgcat agcgcccttg attaccgctg acgttgataa cgccacattg
    13441 cctggcggca aagccagcga tgctgcacaa gattttctcg cgttgttgag cgaagcatta
    13501 gcaggcgaga caacaaccga caaagcggcc ccccagttgc tggtggcaac agataagccc
    13561 acgacaaaag gcgagccgct ggtcagcgat attgtttccg atgcgcaaca agcagattta
    13621 ctgatccctg tggatgaaac accgcctgtc atcaacgaca aacaatccac atcaacatcg
    13681 ttaaccaccg ctcagacgat aacgttggct gcggcggctg acaacaatac ggcaaaagac
    13741 gaaaaagcgg atgatctgaa tgaagacgtc accgcaagcc tgagcgccct ttttgcgatg
    13801 ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt gttaccgaca
    13861 gagaaaccaa cgcttttcac aaaactgact tctgcgcaac tcactacagc acagcctgat
    13921 gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc ccagagtaaa
    13981 gcggaagtca tcagcacgcc ttcgtcggtg accgctgccg ccagcccgct aatcactcca
    14041 caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcacc gctgggttct
    14101 cacgaatggc aacaatcatt aagccagcat atttcgctgt tcacccgcca ggggcaacaa
    14161 agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc cctcaaagtg
    14221 gatgataacc aggcgcaaat ccagatggtt tcaccgcatc agcacgtacg cgccgccctg
    14281 gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca gttagggcaa
    14341 agcaacatca gtggcgaaag ctttagtggt cagcagcagg ccgcttccca acaacagcaa
    14401 agccaacgca cagcaaacca tgaacctctg gcgggggaag acgacgatac gcttccggtt
    14461 cccgtctctt tacaagggcg tgtaacaggc aacagcggcg ttgatatttt cgcctaacgt
    14521 cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag acgcaggata
    14581 attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat tacgcgataa
    14641 gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc ctcgcggcct
    14701 gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgaa gacaaagcgc
    14761 agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg gtcaatctgg
    14821 gcgatgcgga tcgcgtactt tatatcggca taaccctgcg actcaaagat gaagctaccc
    14881 gctcgcggct gagtgagtat ttgccggaag tccgtagccg tttgctgtta ctgttttcgc
    14941 gtcaggatgc tgccgtactg gcgacagaag aaggtaagaa aaacctgatt gccgagatta
    15001 aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc accgacgtgc
    15061 tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc tcaagctgaa
    15121 attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc cagtgttagc
    15181 ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt gcgcgaacgt
    15241 ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat ggggctgttc
    15301 aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca gccgtaccat
    15361 gaatttgccc gcaacttgcc ggtgccgacc aacctgaacc ttatccacct taaaccgctg
    15421 cgcggcacag ggctggtggt gttctcaccg agtctggtgt ttatcgccgt ggataacctg
    15481 tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac ccatacagaa
    15541 cagcgcgtca tcaaccgcat gttaaagctg gcgcttgaag gctatagcga cgcctggaag
    15601 gcgattaatc cgctggaagt tgagtacgta cgttcggaaa tgcaggtgaa atttaccaat
    15661 atcaccacct caccgaacga catcgtggtt aacacgccgt tccatgtgga gattggcaac
    15721 ctgaccggcg aatttaatat ctgtctgcca ttcagcatga tcgagccgct acgggaattg
    15781 ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg cgataacctg
    15841 gtgcgccagg tgcagcattc acagctggag ctggtcgcca actttgccga tatatcgcta
    15901 cgcctgtcgc agattttaaa actgaaaccc ggtgacgtcc tgccgataga aaaacccgac
    15961 cgcatcatcg cccatgttga cggtgtcccg gtgctgacca gtcagtacgg caccctcaac
    16021 ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc tctgaacgag
    16081 gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc gcaatggacg
    16141 atctgtgggc tgaagcgttg agcgaacaaa aatcaaccag cggcaaaagc gctgccgacg
    16201 cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat atcgacctga
    16261 ttatggatat tccggtcaag ctgaccgtcg agctgggccg cacacgaatg accatcaaag
    16321 agttattgcg tctgacgcaa gggtccgtcg tggcgctgga cggcctggcg ggcgaaccgc
    16381 tggatattct gatcaacggt tatttaattg ctcagggcga agttgtcgta gtcgctgata
    16441 aatatggcgt gcggatcacc gatatcatta ctccgtctga gcgaatgcgt cgcctgagcc
    16501 gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttcta ctgcgccact
    16561 gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg ctgcctggct
    16621 ggtaaaacgg ctgggatttg ccccgaaacg cactggcgtt aacggtctga aaattagcgc
    16681 cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag atgcacggct
    16741 ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc cttcagcacc
    16801 aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaacttgc ttaagcgtag
    16861 cgggagatcc tgatgcgtcg tttgttgtct gtcgcacctg tccttctctg gctggttaca
    16921 cccctcgcct tcgcgcaact gacgggtatt accagccagc cgctgcctgg cggtggacaa
    16981 agctggtcgc tcccggtgca gacgctggtg ttcattacct cgttgacgtt tattccggca
    17041 attttactga tgatgaccag tttcacccgc atcatcattg tttttggttt attgcgtaac
    17101 gcactgggaa caccctccgc gccacctaac caggtattgc tggggctggc actgtttttg
    17161 acctttttta ttatgtcacc ggtgatcgat aaaatttatg tcgatgcgta ccagccattc
    17221 agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc gctgcgtgag
    17281 tttatgctgc gtcagacccg tgaggcggat ttaggattgt ttgccagact ggcaaatact
    17341 ggcccgttgc agggacctga agccgtgccg atgcgcattt tgctcccggc ttacgtgacc
    17401 agcgagttga aaaccgcatt tcagataggc ttcacgatct tcattccgtt tttgattatc
    17461 gacctggtga ttgccagcgt gttgatggca ttggggatga tgatggtccc ccccgccacc
    17521 attgctctgc cctttaaact gatgctgttt gtactggtgg atggctggca attgctggtc
    17581 ggttcgctgg cgcagagctt ttacagctag agaggcaaaa tgacacctga atcggtcatg
    17641 atgatgggga ctgaagcgat gaaagtcgcg ctggcactgg ctgccccgct attgctggta
    17701 gcattggtca cgggccttat catcagtatt ttgcaggccg ccacgcagat taacgaaatg
    17761 acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg ccattattat tgccggaccg
    17821 tggatgctca atctgttgct ggattacgtc cgcaccttgt tcactaacct gccgtatatc
    17881 atcgggtagg ccgtacaatg atgcaggtga caagcgatca atggctttcc tggctaagtc
    17941 tgtacttctg gccgttactg cgcgtgttgg cgctgatctc caccgcgccg attctgagcg
    18001 aacgcagcgt accgaaacgg gtaaaactgg gtctggcaat gatgatcacg ttcgctattg
    18061 ccccatcatt acctgccaac gatgttcctg ttttttcgtt ctttgctctg tggctggccg
    18121 tgcagcagat cctgatcggc attgcgcttg gttttaccat gcaatttgcc tttgccgcgg
    18181 tgcgaaccgc tggtgaaatt atcggtctgc aaatggggct gtcatttgcg acgtttgtcg
    18241 atccggccag ccatcttaat atgcccgttt tagcgcgtat catggatatg ctggcgttac
    18301 tgctgttcct gacatttaac ggtcatttat ggttgatttc actgctggtc gatacctttc
    18361 acaccctgcc aattggtggc gaaccgttga acagcaatgc gtttctggca ctcaccaaag
    18421 cagggagttt gattttcctt aacgggctga tgctggcgtt accgctcatt actctgctgc
    18481 tgaccctgaa tctggcatta ggtttactaa atcgtatggc cccgcaatta tccatttttg
    18541 ttattggatt tccattaact ctgactgtcg gtatctcttt aatggcggca ttaatgccgt
    18601 taattgcgcc tttttgcgaa catttattca gtgagatgtt taatttgctg gctgatatta
    18661 ttagtgaatt gccattaata taattccgta acgtttatca tgctacccta aggattatcc
    18721 gaaaaataat acctacgaac atcttccagg atactcctgc ggcgaaatat ttgttttaag
    18781 ctcactcaca tatcgcaaca tttactttac tttaagacaa ttccaggcaa attatacaac
    18841 actttacggg atagtaagtc cgcctgaaaa atcgcgagag tggcgcatta ggtgacccat
    18901 gttgttccgt ttagtcatga tgaaatattc aggtaagggg aattatcgtt acgcattgag
    18961 tgagggtatg ccatgtcaac gattattatg gatttatgta gttacacccg actaggttta
    19021 accgggtatc tgttgagtag aggggttaaa aaaagagaaa tcaacgacat tgaaaccgtt
    19081 gatgaccttg ccatagcttg tgattcacag cgcccttcag tggtgtttat taatgaggac
    19141 tgtttcatcc acgatgcttc taacagtcag cgcatcaagc acatcattaa tcaacatccc
    19201 aatacgttat ttatcgtttt tatggcaatt gccaatgttc attttgatga atatctattg
    19261 gtcagaaaaa atttattgat cagttctaaa tcgattaaac cggaatctct cgatgatatc
    19321 cttggcgata ttctgaaaaa agagacaacg ataacctcgt ttttaaatat gccgacgtta
    19381 tcattgagcc gaaccgaatc gagtatgttg cgaatgtgga tggcaggtca gggaaccatt
    19441 caaatctccg accaaatgaa tatcaaagcc aagaccgttt catcgcataa aggtaatatt
    19501 aaacgtaaga tcaaaacgca taataaacag gttatctacc atgtcgtccg actgacggat
    19561 aatgtgacta atggtatttt tgtcaacatg cgctaacaca ttctgactgg tggtttccca
    19621 ccagtcaggc tgaattagat tactctgctt tctccacaaa gataccgtcc tgatgccctg
    19681 cttcattaaa gaaccagatg ccgagcgggt agtcttccag cgaaaccagg tacattgtgc
    19741 cttcactaaa ctcctcaact gccagtacca cgccaggacg acgcggaccg ccatccgttt
    19801 tgactgttac ccgatcattc accttcatcg ttttcctcct gtggctttgt gccagtgtag
    19861 aacaatttcg tttttctggc agcgccaggc gcgcgcgagt gctgattttc tcgacggtct
    19921 atacttaaga gatgccaggc ggacttaacg actggcggca acaacagagt aacggttgcg
    19981 aggaaagatg atgaaaaccg caaaagagta cagcgatacc gcaaaacgtg aggtcagcgt
    20041 cgatgtcgat gccctgctgg cggcgatcaa tgaaattagc gaaagcgaag ttcatcgcag
    20101 ccagaacgat tctgaacacg ttagtgtcga tggacgtgaa tatcatacat ggcgtgaatt
    20161 ggcggatgcc ttcgaactgg atattcatga cttcagcgtc tctgaagtga atcgttgaat
    20221 gcacaataaa aaaaatcccg accctgaggg ggtcgggatg aaacttgctt aagcaagaag
    20281 cacttaaaaa attcgttaca ccaggaaatc tgatgtgttc atcaccttat ccgcaatttt
    20341 tttcgctgac aagaaaatat tcgtgatatg aatgattaac gctacttatg aatagaaatg
    20401 tgacgcgcgt caccccttgg tggctctggc gtcaacggga tcgctcccaa aaaaatcatc
    20461 tttgataatt tgtgctgttt tgagaatcat cctggtaagg ggagtactgc gcaaccatgc
    20521 tttcaattca acaaccacta ctggttttta gcgatcttga tggcaccctg ctggacagtc
    20581 atagttatga ctggcaaccg gcagccccct ggctcagccg tttacgcgaa gcgaatgttc
    20641 ccgtcattct ctgtagcagt aaaacttcag cggaaatgct gtacttgcaa aaaatgttgg
    20701 ggctacaagg cttaccgctg attgcagaga atggcgcagt gatccagctt gctaagcaat
    20761 ggcaggagat agacggtttt ccacgcatca tctcaggtat tagccatggc gaaatcagcc
    20821 aggttttaaa tacgctacgc gagaaagaac attttaaatt cacgactttt gatgatgtcg
    20881 acgatgcaac catcgccgaa tggacgggat taagccgtag ccaggcggcg ctgacgcagc
    20941 tgcatgaggc gtcggtaacg ctaatctggc gcgacagtga cgagcgtatg gcacaattta
    21001 ccgctcgtct gaacgaactg ggcttgcagt ttatgcaggg tgcgcgcttc tggcacgtcc
    21061 tggatgcctc tgccggaaaa gatcaggctg ccaactggat tatcgcgacc tatcaacaat
    21121 tgtcaggcaa acgcccaacc acacttggcc tgggcgatgg gccaaacgat gcgcccttac
    21181 tggaggtaat ggattacgcg gtgattgtga aagggctaaa ccgtgaaggg gtccatctgc
    21241 atgatgagga tccggcccgc gtctggcgaa cgcagcgtga aggaccggaa ggctggcgtg
    21301 aagggctgga ccattttttc tccgcccgtt aagcgttatc gctcgcgaat acccgattgc
    21361 gcccggcctg ttttgcgagg taaagccgac ggtcggccaa tgactggagt tgttcaaaat
    21421 cataatcacc ggtttcctcg ctgctgctta cccccagcga ggcactgatg cgtatcgtcg
    21481 tactcttggc gatcagcatc tctttttcat ttaacttcag gcgaatacgt tctgcgacct
    21541 ccgcagcctg cgtcagattc gcgcctggca gaatcacaca aaactcctca ccaccgaccc
    21601 gcccggcaac gtcctgcgca cgtaaggaac tgctaattaa tccggcagca tgagaaagaa
    21661 cacggtcgcc cacctgatga ccaaagcggt cattaatcgc tttaaaatgg tcaagatcga
    21721 cctgaatgac agaaaatgga tgttggtgcg tctgacacaa tttagcgagc ggacgggctt
    21781 tttcgaacag tgcgccacgg ttatataaac gcgttaaggt gtcgtgccac gcctgccact
    21841 gcaacgagct ttgcagaaca tacatgttgc tgaccatccg gcgaatcaca taccaggaga
    21901 gtagtaacat ggtggtaaag agcgcccaca gcagggttaa tgcaatgctg atactgccga
    21961 aatcaccacg cacgccttcg cttagcgtat ggacgcgcac cagcacaccg tcgaaatggt
    22021 ccagacgttc ccagctaaca taacgactgt tcatacgaat gccaccccgc gtgtcatgtt
    22081 ccattgcctg cgccaacaag gccagttcac gaggatcaaa aatattccct gttggatggt
    22141 caggattgga agaggtcaaa aatctcagct tactgtcata gagctgatac tcaccatcga
    22201 ggtttttatc gatggcgttt cttaaaaatt gctgcatggt acgcacggga atactcatcc
    22261 ccagcacgcc ataccagtag ttttcactgt ctaccggaac actgacggta acctgcggtt
    22321 cagtattgct ggcgtgttcc ggttgcgaag tgaaccagcg taccgcgcgg tgacgatttt
    22381 ctcgttgcga atggccgata aaccagggtt gggtgacata gccgtaataa cgcgttggta
    22441 cattgcgcgt aaacaaggtc ggctgcgtcg aaacgtaaaa tccggcacgc gagacataca
    22501 tcgcctgttc aaccatcgac gaggagttgt gcgccagtcg cagcaagtaa ccaacttcca
    22561 gtgcagcggt aatttcattg tcgaggcttt cattttcgcg agacaggaga ttcccctcgc
    22621 tgactaatgc atccgacacg ccattgactg gcagggtgcg tcgtcggttg agttcgattt
    22681 gccaggcgtg ctcgtcgcga tgctgttcga actcggttac ggcattacgc agagaggtga
    22741 aatccagtgg cgctacgaga gcttcgcgca tgccattacg caaaaagatc agtttgtcga
    22801 cgttatactg caaatgctta tcgagcgcgt tggctacgtt ttccagatga ttacgctggc
    22861 tggagatata ggcatcttcc agcaccacca cttcacgcca ggtgagcaag gtggaaaaaa
    22921 gcaataccac gataaagcag agattaacga catgaccagg ccccaggcgg cgtgcgagtt
    22981 ttttcaacca gctctggttt tccatttttg tctcgtgctg caccctgacg actcccaacc
    23041 tgctttttta tgattctggc ataacccggc gataacgtac cgcaaaacag cgataatttt
    23101 ataatggtgg catgataaga cgcgactatc attcattgca ggcattggca caccaggccg
    23161 gatgtgatcc ggcctggtgt taatgatacc ctttcaaact tcttccgcag aacgactccc
    23221 ctcccccgga acaagatcgg tttcatgaaa agcttcccgc ttgaccccgt aaccgtcata
    23281 ccaacgacac tcaaccatac cgctggagta tccagtgaca atcatccgcg ggccgccctc
    23341 tttaaccgta acttcctcac taaccataaa gctcatactc gcctcctttt ttcgagtgaa
    23401 acttgttcac cttagttgaa gatggcgaat tttgcataat agccagtgcg agtattaatg
    23461 tgcctgaccg cgcattttcg ctactgcttt cactcccagc accacgatgc cgccgatgat
    23521 aaaaccaaga atcagattta aaacagtcgg taatatcatc gccaccactg cactttgctg
    23581 cccggcgaaa tgttcaatgg cgtgatgcag cggcgcaata ccatgtacca caatcccgcc
    23641 gccgacgagg aacatcgcca gcgtgccgac aatcgataat gctttcatca gccagggcgc
    23701 gataatcaat aaccctttac ctaatgcctg catcagcgcg ctggattttt ccgccagcca
    23761 ataccccagg tcatcaatct taacgataac ccctaccaga ccgtagacgc ccacggtcac
    23821 taccagcgcg atgcctgaaa gcaccagcac ctgattaagc aacggcgcct ctgccacaat
    23881 ccccagcgtg atggcaacga tttccgcaga caaaataaaa tcggtacgaa tcgccccttt
    23941 aattttgtcc ttttcaaact tcagcggatc ctgcgccgcc agcttctcca gacgctgctg
    24001 actctgcgcc ggatcttctt tatgtttacg cgcctccagc atatgcagca ctttctctac
    24061 cccttcaaag cagagaaacg cgccgccaat catcaacagt ggcgtaatcg cccacgggat
    24121 aaacgcactg atgatcagcg ccagcggcac cagaatcact ttattgatca gcgatccttt
    24181 cgccacgccc cagaccacgg gaagttcccg gttggcccgc acgcctgaaa cttgttgcgc
    24241 attgagcgat aagtcatccc ctaatacacc ggcggttttc ttcgccgcca gtttgcccat
    24301 cacggaaata tcgtccagca gtgtggcgat atcatcgagc aacgtcagta aactactccc
    24361 ggccaaaagc attccctctc atttttattg ttgaagggtg taagtatgaa gcaaaaacgg
    24421 cagctctgat acttgcttca caggtcaaca tttttttaac aatcaagtgc aattatatct
    24481 ctcgccagca gaatgatttt cacgtttact atgacccacc tttttatttc agctgtgagg
    24541 gattatgcgt ttccgccagt tgttaccgct ttttggcgcg ctgtttgcgt tgtatatcat
    24601 ttggggctca acctattttg tcattcggat tggcgtggaa agctggccgc cgttaatgat
    24661 ggcgggcgtg cgattcctgg cagccggtat tttattgctg gcatttttgc tactgcgcgg
    24721 acacaaactt cccccgctac gtccgctgct caatgccgcg ctgattggcc tgttattgct
    24781 ggctgtcggt aatggcatgg tgacggttgc cgaacatcaa aatgttcctt ccggcatcgc
    24841 cgccgtagtg gttgcaaccg tgcccctctt taccctgtgc ttcagccgcc tgtttggcat
    24901 taaaacgcgc aaactggaat gggtgggtat cgccattggg cttgccggaa tcatcatgct
    24961 caatagcggt ggaaatttaa gcggcaatcc gtggggcgcg attctgattt taatcggctc
    25021 gattagctgg gcgtttggct cggtttatgg ctcgcgcatt accttacctg tagggatgat
    25081 ggcgggtgcg attgagatgc ttgcggcagg cgtggtgtta atgattgcgt cgatgattgc
    25141 gggtgaaaaa ctgacggcgc tcccttccct ttcaggcttc cttgcggtcg gctatctggc
    25201 gctgtttggt tcgattatcg ccattaacgc ttatatgtat ttaatccgca acgtcagtcc
    25261 ggctctcgcc accagctacg cctacgttaa cccggtggtc gcggtcttac tgggtacggg
    25321 actgggtggc gaaacgctgt cgaagattga atggctggcg ctcggcgtaa ttgttttcgc
    25381 ggtggtactg gtcacgttgg gaaaatatct cttcccggcc aagcccgttg tcgcgccagt
    25441 tattcaggat gcatcaagcg agtaaatgaa tcccctgcgt gtcgatctgc gcgctggcac
    25501 cttcgccgca gatccactct tccagacgct cggtaagcgc cgcatccgta agcttctcgc
    25561 gcccacgtaa cgcacactcc cagacaatca gtacgcgcca gccgagttcc tgcaagcgac
    25621 tgatatcgcg gcgatcgcgt tcaacatttt tacctatctt ctccagccag aactcggttc
    25681 gagtcgcagg cactttaaac agatagcagt gatgatgatg ccagaagcag ccgtgggtaa
    25741 atatcacgca gcgatattca tcaacgacaa aatccgggcg tccgggcaga ctggcgtcct
    25801 gaacgcgaaa cgcaaggccc tgcccggtta acaaactggc gaggcgcttc tcaatcgccg
    25861 tatcacgcgt ggcaatcgcg cgcatatttt tgctgcgggt ggccttatcg tgaacgtcga
    25921 ccatgttgtg cctcttgctg acgcaacgcc accgcctgtt tgatttttgg ctcaagcagt
    25981 tttgccaccg cggcaaagac cggcacgacc accgagttac cgaactggcg ataggcctga
    26041 gtgtccgaaa ccggaatgcg gaatttcact tctcctggcg cttcaaaacc cattaagcgc
    26101 gcacattccc gaggcgttaa ccgacgtggg cgatgttgct gattcagcgg atcgtcaaag
    26161 tctttttcac ccgtggccat atcccagccg cgatcgatta aaatttccgc gccatctttg
    26221 taataacgag cagacagcgt acgcgtgacg ctttgcggat tgttcggata aaccattcca
    26281 taaccgaagc cattaccgcg cgcctgatgt tttttcgcat aacgatagag gtacttccac
    26341 agcaccggcg tcaggatata tttcgcctcg accatcgggt ccagcagctg ggccagcgtc
    26401 actcgctgcg cagggaaaca ttcgctgata tcacgcaggg taaaatcggc tttcagattc
    26461 agatcgcgac gaaaacccac cagcacgatg cgttcacggt gctgcggcag gaaatgtttg
    26521 ccatcgatga ttttcggatc gtccggcccg ttatcttctg catcagccac gtcatagccc
    26581 agttcgtcca gcgtctgcat gatgatgcgg aacgttttac cctggtcgtg acttttcagg
    26641 tttttgacgt tttcgagcac aaacatcgcc ggacgacgcg cgtcgataat gcgcaccaca
    26701 tcaaagaaca gcgtgccctg agtatcgcag gcaaaaccgt gcgcccgccc gagcgagttc
    26761 tttttcgata cgccagccag cgaaaatggc tgacaaggaa aacccgccag taatacatcg
    26821 tgttcaggaa tgtgttgacg aatatgttcc gccgccgcct catcactcac gccttcttta
    26881 tggctgaggg tgatgtcgcg gatatcttca ttaaaatgat gcgtcgccgg gtcgcaataa
    26941 tgattggctt tataagtgcg taccgcatgt ttgttccatt cgctggtaaa cacgcactgt
    27001 ccgccaatcg attcaaaacc gcgacggatg ccgccaattc cggcaaatag atcgataaag
    27061 cgaaacgcat aatgcggatg atgtgccggt ggtttgggta acaacgtttg cagatgggcg
    27121 aactctttct cacttaaacg gtgccatgcc gagtcattcg ccagcgcacg ttttaaaatt
    27181 gccgcgctcc agtgattctc acccacacca ttaagctgcg ccaccagcgt tttaacatca
    27241 taaatttgca gcagtttctc cagcattgcc tgaacgacat tcccggtgct gtatgaatcg
    27301 gttactgata tattttcctg catagattta accggccatc tgagatggcc gacaggttaa
    27361 cataattaca gccatttagg gccacgcgct cagcccgcgg gtgaaaacgc atctatcacc
    27421 ttgtgatcga caccttcatt ctcccccgcc agttcggcac tgagcttcgc cataaactcg
    27481 actaaaaatt gcgcgttgtg ctgcgccagc tgcttgcctc ttgcggtttg catggtttgc
    27541 ggtagcttca gcaacttagt ctggaaatga tccagcgcgt agcgtttatc atccagcggg
    27601 cgatgctgtg cgaacggatc ttcaccatca aacagcgcca cgcccaatgc ccctgaaaca
    27661 gcaaacacgc gcgccaggcc aatcgccccc agggcttcca gccgatccgc atcctggacg
    27721 attttagctt ccgttgttaa gggggcgatt tgcgcgctga aactgtgagc ggcaatggca
    27781 tgacaaacgg cctcgatttt ctccgccgga aattgcacga actcttcacg gagcaggcga
    27841 cgcgcctctt ctgccgccag gattgaagaa tgctgccgtt gcggatgatt tttcgccagg
    27901 ctcacaatat cgtgaaaata acaggcggtt aaaatcacca gcatatcgac atcgtcatct
    27961 gccgccagct tctgtgccgt agcccagacg cggcgaaaat gacatacgtc atgggccgcg
    28021 tcctggtgct gatgatggtt tttcaaccag ttttcgaact gtgcctgcca gtgttgtaag
    28081 tccatacgcg ctccgttgcc aaagagtcgc aaccttagca gtttttattc atagcgtctg
    28141 ctacgtgcgg gacgaggtcg ggtgtaccag gcaatgctgc ctaacaatgc cccgaccaga
    28201 gccataaata aaaatccgac cagtgcccct aaccactttc ccgcggtaga gcctaaatcg
    28261 ctttcaaatc ccgacaccgg ggcatcaggg atccggctca tcacccagat gtaacgcgcc
    28321 attgcccaga cgaaatagcc acaccatgta tagaaagccc acaaagccaa tttgccgcca
    28381 gggctgcgag agagtctctc ttccatcttt gtcgaaccgt attccatttt gagataaccc
    28441 ggaaaattca gacttagtac tattatgtga taaaagtcac atttttccac attggatgag
    28501 ccagtcaaga ctcaatcatt ttttgaacaa atagataatt tttgcgcaac ggtagtgaat
    28561 acaggaaaaa tcgttttgag cgattttatt tatctttgat atcggcttta ttaattcatt
    28621 caatcaatat attagcactg attacaatta taccttttta ttattctgtg tcatgatgcc
    28681 tccgttatta gccttttatc gtcttgttta tattttttgg gccggcatga tgccggcttt
    28741 tttttattcc ttcattaatg tgcgcgtgat cacaccagcc gtttggcgca acaatcattg
    28801 atacccccta tgtttccgct caactcataa catattgata tgcattcata atattcgtga
    28861 atatttatat tcagaattca taattatgaa ttatattaaa
//
LOCUS       DAZGHF010000054        28840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGHF010000054 DAZGHF010000000
VERSION     DAZGHF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.054"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..9690)
                     /gene="cdiA"
                     /locus_tag="ACHK3A_002448"
     CDS             complement(<1..9690)
                     /gene="cdiA"
                     /locus_tag="ACHK3A_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition toxin CdiA"
                     /protein_id="HFO8740513.1"
                     /translation="MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GKPVMNADGSLQALEVTEGSITINGAGLDGTWSDAVSIIARATEVNAALHAKDLTVTA
                     GANRITADGRVSALKGEGNVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARE
                     GDIILSSSGKLVLKNSLAGGSTTVTGTDVSLSGDNKAGGNLSVTGTTGLTLNQSRLVT
                     DKNLVLSSSGQIVQNGGELTAGQNAMLSALHLNQTSGAVNAAENVTLTTTDDTTLKGR
                     SVAGKTLTVSSGSLNNGGTLVAGRDATVKTGTFSNTGAVQGNGLKVTATDLTSTGSIK
                     SGSTLDISARNATLSGDAGAKDSARVTVSGTLENRGRLISDDVLTLSATQINNSGTLS
                     GAKELVASADTLTTTEKSVTNSDGNLMLNSASSTLAGETSAGGTVSVKGNSLKTTTTA
                     QTQGNSVSVDVQNAQLDGTQAARDILTLNASEKLTHSGKSSAPSLSLSAPELTSSGVL
                     VASALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSGLI
                     TGDNGLTLNTASLSNPGKITADTLNVRATTLDGDGLLQGAAALALAGDTLSQGRHGRW
                     LTAGDLSLRGKTLNTAGTTQGQNLTVQADRWANRGSVLATGNLTASATGQLTSTGDIM
                     SQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTLHHRSTDNSGTVTGL
                     SGLTLHSADGLTNSGALLSQNSLVLSAGDVTNSGRIQGQNITLDASSLTSSGAVQSAL
                     DLALTLSGDVIAATGSKITALGDARLSGKVLGNQGLISAKTLEVNGDSLSNSGEISGV
                     NSLNVTLSGNLQQHGKMLTGGALNVNARDISNSGQLQGADNRITASSLANSGRVQGES
                     GLTLTLLNALTNQTSGVLLSQNVSALSAPALTNDGTIQGNGKTTLSAATQAHNSGKIL
                     SGGELTFTTPDYSGSGWLQATDLLLNVAKLAGNGTVMAANQATLTGNSLTNRGLFQAA
                     QLNVNTQTITNSGTLLGNQGLTIKGNNLNNAGGKVFSGGDMLAEMVSLSGAGQLVALG
                     NLTLKLTRGLTAQGVIAANKQLSVSSQGDITNGATLQGNGITLNAAGRLTNNGQLTAG
                     NGTTALSGSGIAMNASGSLQAGGDVSLTSRGDITLDAFTGTTGSLMLTAAGAVINTAL
                     LYAGNNLSLFASTIRNHHGDMLAGDSLVMQKDVSGAANAEVINTSGNIETTRGDITIR
                     TGHLLNQREGINETKSYIPVENVAVPDGANSVSVRVGDLGEDGWGYYVKSWSGTAGGG
                     FDAWAVPTEKGATRKFLTGTTRVDVGATGGDARISAGNNLLIDADKLDNTGSHLLASG
                     FVSLSGSQLNNQSFFGYTQDEYNVYRYYGKLAMIPNDGHLQYGDASADDRVTFTLSGA
                     PEYVTRDTGQALRAVIQAGKNVTAVFSSDISNTSTTSNAGRITNTLAAPEINTPAEKN
                     ISPGMAQLAPDGTETLTVTAPDWTDTITRLTIGSGTDLASGIVEGNYPLPSGNNGYFV
                     PSTDPDSPYLITVNPKLDGLGKVDSSLFTGLYDLLRMHPGQAPRETDPAYTDEKQFPG
                     SSYFLDRLGLKPEKDYRFLGDAAFDTRYVSNYMLNQIGGRYINGVGSDTDQMRYLMDN
                     AARAQKALGLKFGVALTADQVAALDQSILWYKAVTIKGQTVMVPEVYLSPKDVTLQNG
                     SIISGQNVHLAGGNVTNSGSTLMAQNNLTIDSADSLGNLESGLINAGGALGLKAMGDI
                     NNISATITGKTVRLESLAGNVNNLTRYSHWQLDAPEDSLALKHTYTGSIASVSAMDSL
                     DIRADKNISVTGAEISAGDRAALIAGNDLSLNAIDRVSSRRHANSESHQRSAGLTTIT
                     AGDSVMLSAGRDVSSQGAGIAAEDNITVRAGRDVNLLAEESVTGSSSYSKKKTVIDET
                     VRQQGTEIASGGDTTITAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLET
                     KKKSGGFLSKKTTHTISEDSATREAGSLLSGNRVTVNAGDNLTVEGSDVVADRDVSLA
                     AGNHVDVLAVTSTDTSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSAS
                     TIGSTAGNVSITAGKQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSG
                     LTVALSGTVGSAINNAVTSAQETKESSDSRLKALQATKTALSGVQAGQAAAMATATGD
                     PNATGVSLSLTTQKSKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGS
                     QLKAGGNTSLDAANDILLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVN
                     AAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQ
                     DTSKYDSKQTSVAAGGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDI
                     TVGRHTQLDGAVIASTATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDK
                     FQGNMPGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQNPADLSRDPAHANDSI
                     SPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQ
                     RQEYLAKLRDTPEYKKEQEKYGTGSEIQRGIQAATAALQGLAGGNLAGALAGASAPEL
                     AHLLKSTEKDPVVNAIAHAILGGAVAAMQGNNVAAGAAGAATGEMAARAIAGMLYPGV
                     KQSDLSEEQKQTISTLATVSAGLAGGLTGNSSASAAVGAQSGKNAVDNNYLSVSEKTE
                     LEIAKQTLKNSKNPAEREKAQQKYDALLEKDIASDKEVIAACGNGNAGSSACASARLK
                     VIASKEGYEDGPYNSKYSQQYADAYGQIVNLLDITSVDVQNQQQVKDAMVSYFMATLG
                     VDQKTAQGYVETTQGLEIAAASMTPLFGQAVANKITALVDKANKYPSGIGFKINQPEH
                     LAQLDGYSQKKGISGAHNADVFNKAVVDNGVKIISETPTGVRGITQVQYEIPTKDAAG
                     NTTGNYKGNGAKPFEKTIYDPKIFTDEKMLQLGQEAAAIGYSNAIKNGLQAYDA"
     gene            complement(9703..11481)
                     /locus_tag="ACHK3A_002449"
                     /pseudo
     CDS             complement(9703..11481)
                     /locus_tag="ACHK3A_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            11981..12370
                     /locus_tag="ACHK3A_002450"
     CDS             11981..12370
                     /locus_tag="ACHK3A_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740514.1"
                     /translation="MKRYIKWLAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKN
                     AILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDNSLNLQASDG
                     KIHKNYNRWVRNLDKDIQVNLSTGATL"
     gene            12434..13492
                     /locus_tag="ACHK3A_002451"
     CDS             12434..13492
                     /locus_tag="ACHK3A_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO8740515.1"
                     /translation="MYEQDTLSTLDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEQTVLNEYAVSVLLDMGLSGRIITCKEGNYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARASWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFQAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            13533..14255
                     /locus_tag="ACHK3A_002452"
     CDS             13533..14255
                     /locus_tag="ACHK3A_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO8740516.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRYQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALMIESGDDWQCETQSAIAVNETTLPQSMLFLQH
                     YLQNAGAFSLSGERVQWRWSRR"
     gene            14252..15073
                     /locus_tag="ACHK3A_002453"
     CDS             14252..15073
                     /locus_tag="ACHK3A_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO8740517.1"
                     /translation="MNKLMSRLGWAWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVHGRVEINKFYDATSHEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            15048..15305
                     /locus_tag="ACHK3A_002454"
     CDS             15048..15305
                     /locus_tag="ACHK3A_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO8740518.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTNAPLFGDGLGLDSIDA
                     LELGLAVKNEYGVVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            15317..15568
                     /locus_tag="ACHK3A_002455"
     CDS             15317..15568
                     /locus_tag="ACHK3A_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO8740519.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVKRLLQEG"
     gene            15573..16154
                     /locus_tag="ACHK3A_002456"
     CDS             15573..16154
                     /locus_tag="ACHK3A_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740520.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFLIWLGLAHNSLHWLSPLMALL
                     LLLRLRQPRRQAGPLQAVTKIVAVVGIVLCVSSFLLKTHQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRCQMIKRKTP"
     gene            16151..17509
                     /locus_tag="ACHK3A_002457"
     CDS             16151..17509
                     /locus_tag="ACHK3A_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HFO8740521.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCILSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNNMNKRVYAQLQELFHD
                     TP"
     gene            17496..17849
                     /locus_tag="ACHK3A_002458"
     CDS             17496..17849
                     /locus_tag="ACHK3A_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO8740522.1"
                     /translation="MIRHEIERHQVQLQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            17840..19516
                     /locus_tag="ACHK3A_002459"
     CDS             17840..19516
                     /locus_tag="ACHK3A_002459"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO8740523.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVAEQPGVTLIRLAENTGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKYATIGKRMDFDTEVMVRLYWQGNTCYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTAARARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGHDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQRGKLHIHCETFADP
                     LLLPRGERQQGLQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            19520..19942
                     /locus_tag="ACHK3A_002460"
     CDS             19520..19942
                     /locus_tag="ACHK3A_002460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO8740524.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVEEQSRELCFVSPDILFERMGVKP"
     gene            19939..20544
                     /locus_tag="ACHK3A_002461"
     CDS             19939..20544
                     /locus_tag="ACHK3A_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO8740525.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLSDDERQRFAAQ"
     gene            20513..22831
                     /locus_tag="ACHK3A_002462"
     CDS             20513..22831
                     /locus_tag="ACHK3A_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO8740526.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVVLLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILMIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLGRLETFTTSLEKA
                     KKDGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALQQEIASYYPGGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            22828..23412
                     /locus_tag="ACHK3A_002463"
     CDS             22828..23412
                     /locus_tag="ACHK3A_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO8740527.1"
                     /translation="MIKSTFWRALALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLSGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAKG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPTGWTLRDNGNKRELRNASGKL
                     VTEIIYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            23414..24583
                     /locus_tag="ACHK3A_002464"
     CDS             23414..24583
                     /locus_tag="ACHK3A_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO8740528.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTNGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDIG
                     YINLHGTATQLNDQIESMVVNALFGKRIPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            24580..25044
                     /locus_tag="ACHK3A_002465"
     CDS             24580..25044
                     /locus_tag="ACHK3A_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO8740529.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            25044..25775
                     /locus_tag="ACHK3A_002466"
     CDS             25044..25775
                     /locus_tag="ACHK3A_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO8740530.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            25772..27001
                     /locus_tag="ACHK3A_002467"
     CDS             25772..27001
                     /locus_tag="ACHK3A_002467"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO8740531.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARSATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(27175..27444)
                     /locus_tag="ACHK3A_002468"
                     /pseudo
     CDS             complement(27175..27444)
                     /locus_tag="ACHK3A_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27841..>28840)
                     /locus_tag="ACHK3A_002469"
     CDS             complement(27841..>28840)
                     /locus_tag="ACHK3A_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8740532.1"
                     /translation="RVTLQEVANFAGVGTMTVSRALRTPEQVSDKLREKIEQAVEELG
                     YIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQLLLGCHEYNQL
                     KEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPFSPAQITIETAF
                     FEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQNTDLVITMPDA
                     ASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSD
                     NCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
BASE COUNT         6304 a         7982 c         7532 g         7022 t
ORIGIN      
        1 tgcatcatat gcctgtaatc cattttttat tgcattggaa taaccaattg ccgctgcctc
       61 ttgaccaagt tgcaacattt tctcatctgt aaatatttta ggatcataaa tagttttctc
      121 aaaaggcttg gcgccatttc ctttataatt accagtcgta ttcccagcag catcttttgt
      181 ggggatttca tactgaacct gagtaatacc tctgactcct gttggtgttt cagaaataat
      241 tttcacacca ttatcaacca cagctttatt gaatacatca gcattatgtg caccgctaat
      301 tcctttcttc tgcgaatatc cgtcaagttg agctaagtgt tccggctgat taattttaaa
      361 accaatccct gaaggatatt tattcgcttt atcaaccaat gcagtaattt tattagccac
      421 agcctgacca aataatggtg tcattgaagc cgctgcaatt tctaatccct gcgtcgtctc
      481 gacataacct tgtgctgttt tctggtcaac ccccagcgtt gccatgaagt aactcaccat
      541 cgcatctttc acctgctgtt gattttgtac atcaacactg gtgatatcga ggagattgac
      601 tatctgacca tacgcatcag cgtattgctg gctgtatttc gagttataag ggccatcttc
      661 gtacccttct ttcgacgcaa taactttcag tcttgcgctg gcacacgctg aactacccgc
      721 attaccatta ccgcaggcag caataacctc cttatcactg gcaatatctt tttcaagcaa
      781 tgcatcatat ttctgctgtg ccttttcacg ctctgccgga tttttactgt ttttaagcgt
      841 ctgttttgct atctcaagct ctgtcttttc agacacgctc aggtagttat tatcaacagc
      901 attcttcccg ctctgtgcgc ccactgctgc tgatgcgctg ctgtttccgg taagaccgcc
      961 agccagccct gctgacacgg ttgccagcgt acttatcgtc tgcttctgct cttcactgag
     1021 gtctgactgt ttcacgcccg gatacagcat gccggcaatc gctctggctg ccatctctcc
     1081 cgttgctgcg cctgctgcac cggcagcaac attattaccc tgcattgccg ctactgcgcc
     1141 acccagaatg gcgtgagcaa tggcatttac taccggatct ttttctgttg atttcagcag
     1201 gtgcgccagt tccggggccg aagctccggc cagagcaccc gctaaattcc ccccagccag
     1261 tccctgaagc gcggctgtcg cggcctgtat accccgctga atctcactgc cagtgccata
     1321 cttttcctgt tcctttttgt attccggcgt atcacgcagt ttcgcaagat attcctgacg
     1381 ttgtttctct gttgcattag caggaagtgt ttcgccggtt gcttccttcg cggccttcaa
     1441 cgcattcagt tccccctgcg tccgcgcgat atccgccacc tgactgccta tgtcactgat
     1501 aagccccact gtctgcagac gcctctgctc cttctccttg tcaaatatcg ggctgatact
     1561 gtcattagcg tgcgcaggat cacggctgag gtctgccgga ttctgcttct gattgtccct
     1621 gtcccggatg gtgatagtgc cttctgccac tgcggcctga gtcgttcctt ccgcatgtcc
     1681 gctgtgacct ccggcggata tcatgccacc cggcatgtta ccctgaaatt tatccccgaa
     1741 gctgccacca ccgctcagac tgatcccact gtgactgact ttataatccg cttcgttgtg
     1801 aaggtcactg aaccccagcg ttccggtatc caggtggttt ttatccggtg tggcagtgga
     1861 ggcaatcacc gcaccatcca gttgggtatg tctgcccact gtgatgtcga agccaccatc
     1921 accggcaaac attccggttt gttcagcaac ggagtcaaag cggctcttca tcttatcccg
     1981 ggaggcagcg atgtaacctg agccggtcat ggagccaaag gtaaaactgc cgccggcagc
     2041 cacgctggtc tgtttactgt cgtacttact ggtgtcctgc tggctgctaa tcagcaggtc
     2101 gtggcccaca tcggcgataa tcctgttgcc gttgacctga gcaccgttca gtaccgtatc
     2161 ccgaccactg ttgatggtga cggttttacc gctgtctgtt gtggtttcag tccactcagt
     2221 accgttacct ttctcgctgc cttttgccgc attaacgctg gcaaagacgc tgataccggc
     2281 accgttacca ccggcaccga tactgacacc cacgccaccg ccactgctgc tgttcctgcc
     2341 cgttgttttt tgtgtgtttg cagcgccact caacagaata tcattcgcgg catccaggct
     2401 tgtgttacca ccggccttaa gctggcttcc tgcaatcaca atatctccgc ggttatcgcc
     2461 cctgtttttg ccggttgcga caacagacag attattcccg gcattcagcg tactgccgga
     2521 tactgtgtca ctttcagaat gttgttgtga tttcgatttc tgagtggtga gcgacaggct
     2581 gactcccgtc gcattcgggt caccggttgc ggtggccatt gccgcagcct gtccggcctg
     2641 cacaccagac agcgctgtct ttgtggcctg cagggctttc agacgactgt cactgctctc
     2701 cttcgtttcc tgtgcactgg tgaccgcatt attgatggca ctgcccactg tgccggaaag
     2761 ggcaaccgtc agcccgcttt tcttctgctc aaatttttcg tccacagtac gacggtcatg
     2821 ccccgggtca accaccacac tgtcaccggt aatgctgata tcccggttcg caatcacatc
     2881 cgaaccgctg atatgagcct gtttgcccgc ggtaatactg acattaccgg cagtggagcc
     2941 gatggtactg gcactctgac tctgcgttgt cccggcctcg cggcggtcgt gcgttgtctt
     3001 actgctgccg atggtgaagc cgataccgcc agtacccatc agacccgatt tcttcgtttc
     3061 cttaaagcgc caggacgtat ctgtactggt gacagcaaga acatcaacat ggttacccgc
     3121 cgccagtgac acatcccggt cagccaccac atccgaaccc tctaccgtca ggttatcacc
     3181 ggcgttaacg gtcacgcggt tccccgacag cagggagcct gcttcacggg tggcactgtc
     3241 ctcactgatg gtgtgggtgg ttttcttact gagaaaacct ccgctttttt tcttcgtttc
     3301 cagatagtga tagtcacttt ctgtcgccgt ggtcagggca acatcacgac cggcattcac
     3361 gctgatattg ccggttgcgg taacggatga cgcaacagcg gtgatatccc gtcctgcagt
     3421 aatggtggtg tcaccaccgc tggcgatttc cgttccctgc tggcgaacgg tttcatcaat
     3481 gaccgttttc tttttcgagt atgaactgct gccggtcacg gactcttccg ccagcagatt
     3541 aacgtcgcgc ccggcccgga cagtgatgtt gtcttccgct gcgatacctg caccctgact
     3601 gctcacatcc cggcctgcac tcagcatcac actgtcgccg gcagtaatgg tcgtaagccc
     3661 ggcgctgcgc tggtgagact cactgtttgc atgacgtctg ctgcttaccc tgtcaatggc
     3721 attcagactg agatcattgc cggcaataag tgctgcgcgg tctccggcac tgatttctgc
     3781 ccctgtgacc gaaatgtttt tatctgcccg gatgtccaga ctatccatgg cactgacaga
     3841 ggcaatgctt cccgtataag tgtgtttcag ggcgaggctg tcttccggtg catcaagctg
     3901 ccagtgtgag taccgggtga gattattgac attgccggcg agactctcca gccggacagt
     3961 cttaccggta atcgtggcac tgatattgtt gatatcgccc atcgctttca gtccgagagc
     4021 gccaccggca tttataagcc cggactcaag gttacccaga ctgtcagcgc tgtcgatggt
     4081 gagattattc tgcgccatca gggtactgcc actgttagtg acattccctc ctgccaggtg
     4141 cacattctga ccgctgataa tactgccgtt ctgcagcgtc acatctttcg gtgacagata
     4201 cacctccggc accatcaccg tctgaccctt tatcgtgacg gctttatacc acagaatact
     4261 ttggtcgaga gccgccacct ggtctgctgt cagtgccaca ccaaacttca gaccgagcgc
     4321 cttctgtgca cgggcagcgt tatccatcag gtagcgcatc tggtcggtgt ctgaaccaac
     4381 cccgttgata tagcgccccc cgatctggtt cagcatatag ttcgatacat agcgggtatc
     4441 aaaagcggca tcccccagga agcgataatc tttttccggt ttcagcccga gccggtcaag
     4501 aaaatacgat gagcccggga attgtttttc atcagtatac gccgggtctg tttcacgtgg
     4561 tgcctgcccg gggtgcatcc tgagcaggtc atacagtccg gtaaacagac tgctgtccac
     4621 ctttccgaga ccatccagtt tcggattcac cgtaatcaga tatggactgt cagggtccgt
     4681 ggacgggaca aaataaccat tgttaccgga aggcagggga tagttcccct ccacgatgcc
     4741 ggaagccagg tccgtgccac tgccgatcgt cagacgggta atggtgtcag tccagtccgg
     4801 ggctgtgacg gtcagcgtct ctgtgccatc cggcgccagt tgagccattc ccgggctgat
     4861 atttttctct gccggcgtgt taatttcagg tgccgcaagg gtatttgtga tccgtcctgc
     4921 atttgaggtg gtggatgtat tactgatatc actggaaaaa acggcagtga catttttgcc
     4981 tgcctgaatc acggctctca gagcctgacc ggtgtctctg gtgacatatt ccggagctcc
     5041 ggagagagta aaggtaacgc gatcatcggc agaagcatct ccatactgca gatgcccgtc
     5101 gttggggatc atggccagtt tgccgtaata gcgatacaca ttgtattcat cctgtgtata
     5161 gccaaagaag gactgattat tcagttgtga acccgacagg ctgacaaacc cgctggcaag
     5221 aagatgactt cccgtattat ccagtttgtc cgcgtcaatc agcagattat tcccggccga
     5281 aattctggca tccccccctg ttgcaccaac atcgacgcgt gttgtgccag tcagaaattt
     5341 ccgggtggct cccttttcag tcggaacggc ccatgcgtca aaaccaccac cggcagtacc
     5401 gctccaggac ttcacataat atccccatcc atcttcacct aaatcgccca ctctgactga
     5461 aacagaattt gcaccatcag gtactgcgac attctcaacc ggaatgtatg attttgtctc
     5521 atttatgccc tcccgctgat tcagtaaatg tccggttctg atggtgatat caccgcgggt
     5581 ggtttcgata ttcccggagg tattaatcac ttcagcattc gcggcaccac tgacatcttt
     5641 ctgcatcacc aggctgtcac ccgccagcat atcgccgtga tggttccgga tggtgctggc
     5701 aaacagcgag aggttattgc ccgcatacaa cagtgcggtg ttaatgactg ccccggcggc
     5761 cgttaacatc agactacccg tcgtgccggt aaaagcgtcg agagtgatat ctcccctgct
     5821 ggtcaggctg acgtccccgc ctgcctgcag gctgccgctg gcattcatcg caatcccgct
     5881 gccgctcagt gccgtggtgc cgttgccggc ggtcagctgg ccattgttgg tcagtctgcc
     5941 tgcagcattc agcgtgatac cgttgccctg caatgtggcg ccattggtaa tatccccctg
     6001 gctgctgacg gagagttgtt tatttgcggc gataacgccc tgtgcggtca aaccacgggt
     6061 gagcttcagc gtcaggttac cgagtgcaac cagctggccc gcgccgctga gcgaaaccat
     6121 ttcagcaagc atatcaccgc cgctgaacac cttcccgccg gcgttgttca ggttgttccc
     6181 ttttattgtc aggccctgat tacccagcag cgtgccgctg ttggtgatgg tctgcgtgtt
     6241 gacgttcagt tgtgcggcct ggaacagacc acggttggtc agactgttac ctgtgagcgt
     6301 tgcctgattc gccgccatca cagtgccatt accggcaagc ttcgccacgt ttagcagcag
     6361 gtcagtggcc tgtagccagc cactgccact gtagtccggc gtggtgaacg tcagctcacc
     6421 gccggaaaga atcttgccac tgttgtgggc ctgcgttgcc gcgctgagcg tggttttacc
     6481 gttgccctga atggtgccgt cattggtcaa cgcgggtgcg gaaagcgctg atacgttctg
     6541 gctgagaagc actccgctgg tctggttggt cagggcattc agcaatgtaa gagtcagccc
     6601 gctctcgccc tgaacacgac ctgagttggc cagcgaactg gcggtgattc ggttatccgc
     6661 cccctgaagc tgcccactat tgctgatgtc tctggcgttg acgtttaatg caccgcccgt
     6721 cagcatttta ccgtgctgtt gcagattacc actgagtgtc acgttcaggc tgttaacccc
     6781 actgatttcg ccactgttgc tgagcgaatc gccattcact tccagcgtct tcgcgctgat
     6841 taatccctgg ttacccagca ctttgccgct cagacgggca tcgcccaggg cggtaatttt
     6901 gctgccggtg gcggcaataa catcgccact cagggtcaga gccaaatcca gcgcactctg
     6961 aacagcaccg ctgctggtca ggctgctggc atccagagtg atgttctgcc cctgaatccg
     7021 gccgctgttg gtcacatctc ctgcgctcag caccagcgag ttctggctca gcagcgcgcc
     7081 actgttggta agcccgtccg cgctgtgcag ggtcagaccg ctcagccccg tgaccgtacc
     7141 gctgttgtca gtgcttctgt ggtgaagcgt gacatggtta ccctggacag tgccgctgtt
     7201 atccagtgag tttccatcaa gggagagcgt gccggccgaa agcagactgc cccggttgtc
     7261 cgtggtggct gcgttcagcg tggtgtcacc ctggctcatg atatcgccgg tactggtcaa
     7321 ctgaccggtt gccgaagcag taaggttacc ggttgccagc acggaaccac ggttcgccca
     7381 tctgtccgcc tgcacggtga gattctgtcc ctgcgtggtc cctgcggtat tcagtgtttt
     7441 accccggagg gagaggtcgc ccgccgtcag ccagcgtccg tgacgaccct gtgagagggt
     7501 gtcgcctgca agcgccagtg cagcggcgcc ctgcaacagg ccatcgccat ccagcgtggt
     7561 cgccctgacg ttcagcgtgt cagcggtgat tttacccgga ttgctgaggg aggcagtgtt
     7621 taacgttaaa ccattatcac cggtgataag cccgctgttg cggatgtccg gtatatccag
     7681 cgtcaggtct gcagcactgt acagcgtgcc gttctgctga ttatcaagcc tctgtgtgtt
     7741 aacggtgagt gaggcctccc cctgcaacag accgctgttg gtcagggtct gtgactgtgt
     7801 attcagggcg gaagcaacaa gtacgccgct gctggtcagt tccggcgcac tgaggctgag
     7861 cgacggggcg ctgcttttcc cgctgtgggt gagcttttca ctggcgttca gggtgaggat
     7921 atctctggca gcctgtgttc cgtcaagctg tgcgttctgc acatccacgc tgacactgtt
     7981 gccctgcgtc tgcgcagtgg tcgtggtctt cagactgttg ccttttacag acaccgtgcc
     8041 acccgcactg gtttcacccg ccagtgtgga agacgcgctg ttcagcatga ggttaccgtc
     8101 actgtttgtg accgattttt ctgtggtggt cagtgtgtct gcagaagcca caagttcctt
     8161 tgccccggag agggtaccgc tgttgtttat ctgcgtggca ctgagcgtca gcacgtcatc
     8221 gctgatgagt ctgccgcggt tttcgagtgt accgctgacg gtaacgcggg cactgtcttt
     8281 tgcaccggca tcaccggaca gtgtggcatt gcgggcgctg atatcgagtg tgctgccact
     8341 tttaatactg ccggtgctgg tcaggtcagt ggctgtgact ttcaggccgt tcccctggac
     8401 ggcaccggta ttactgaatg tcccggtttt caccgtggca tcgcgcccgg caaccagtgt
     8461 cccgccgttg ttcaggctgc cggagctgac ggtgagtgtt ttcccggcaa cgctacggcc
     8521 tttcagtgtg gtatcatcgg tggtggtaag ggtgacattt tcagctgcat tcacggcccc
     8581 ggaagtctgg ttcaggtgca gtgcactgag catggcgttc tgtccggcag tcagttcacc
     8641 accgttctgt acaatctgcc cggatgaaga cagcaccaga tttttatccg tcaccagacg
     8701 ggactgattc agtgtcagtc ccgttgtccc ggtaacgctg agatttcctc cggctttgtt
     8761 atcccctgaa agtgagacat ccgttccggt tacggtggta ctgccgccgg caaggctgtt
     8821 cttcaggacc agttttccgg aactgctcag tatgatatcg ccctcgcggg cataaaggtt
     8881 accaagatta accccgacac cactttcagt ggaggtcaga tgaatacgcc tggcgtacat
     8941 tccaccgaga gcgccggtat caacggcaac tttcggcaca ttaccttcgc ccttcagggc
     9001 actgacgcga ccatctgcag ttatccggtt agcgcctgca gtgacagtta aatccttcgc
     9061 atgaagcgcg gcattcactt ccgttgcacg ggcaataatg gatacggcat cgctccaggt
     9121 gccgtccagg cccgcgccat tgatggtgat actgccttca gtcacctcca gcgcctgcag
     9181 gctgccgtcg gcattcatca caggtttgcc tgtggtgagc gtcgcgtgcg gcgtgttgat
     9241 aaaaccacag ccgtcacagg tgataccata cgggttggca accatcacat tcgccgcttt
     9301 gccggccact tccgtatagc cctgcagcaa tgaacggtta ccgccagtca cttcgttgat
     9361 gatacccttc gcttccccgc ccgctttcag gttcgggtta ttctgtatca gtccaccaag
     9421 ctgcgtcgga ttaagcttac cggtggcatt attgagaatc agcccttcct tcccgacgtt
     9481 gtaatccgta aaccggttat gcgaaatccc ggccccgttc ggcgtggcaa tgttcacgac
     9541 cggcacacca tttgccgctt tatccattcc ggccccgttt tgtggggtga tgacggcccc
     9601 cacagccggt aacaacggct gcccggcgat aatcgtactg ataaggtaac tcagcaggcg
     9661 gtaagtgaag cgaacgggag gctgatgcat ggtaataatc ccttaaaacg cgacggcaac
     9721 gcgccagtaa accgtgagat ggtcaggggc aagccagtcc gggtaaacca gaggcagtcc
     9781 ggcagtgaac gaaccggaaa catggccact ggtggtgctg agcccggcag cagcacccca
     9841 cagcgtgccg gacgagtaca ggtcatctct gtcagagtgc agccagccgc cgtccagtgc
     9901 agtcactgca cggactgtcc ccacatatgg cagggagaac agagaccagg acagctcatt
     9961 tcgcagataa ccgccgttat taccggagat atactgctcc ttaaagccac gcactgaact
    10021 ctcacccccg aggctcagtt gttccacacc atgaagacgg tccggtgacc actgggcata
    10081 agcgctggtc agccaccaca ccctgtccgt gacggggcgc tgaaaactgg cgcttaatga
    10141 ccatttccgg aactgattta cgggcaggtc tccccttttc ccgtggtcgc tttctgcgcc
    10201 gaaccagggc atcccccgtg tgaataccgg attcagtgtt ccgacacccc ccagaaactt
    10261 gtgtgtgtga ttcagcccga cagaaaatga agtgagttta cggctgctgc cctgaagcag
    10321 aacatcatcc agataattgt gaataatgcg gtgctgcaga cctccggtca gtgctgtctt
    10381 catgtccccg ttacggaaca ggacatgcga cagtcccagc cggtgagtct gcaggtctcc
    10441 cgtggaacgc caccgccagc cccggttatc aatggtgctg aggtagtcac tccatgaata
    10501 cgtgtaatcc accagggtat agccatacgg cagactgaca ccggcggcaa aattcctcgc
    10561 atcatgtgac accgaaaagt cactgctccg tcccccgctg acaaaccagt tgtcagccag
    10621 ccccagcgga ttattaaagg aaaggacacc atttaactgc cccgtaccgg tattcttctg
    10681 tccgctgttg tcgatgccca cgctccctgt gacaggccat tccggcgatg ccgtcagtgt
    10741 caccaccgac catccctcac ggtcaccggg cgatatttaa atctgtaccg gctccgtacg
    10801 cagacggtta atctgctcca tcccctgctc aatatcccgc aggttcagaa ccttcccctc
    10861 cattcccggg aaaaccatct tcagggtgcg gccaggaagg tcagcgcctt ccgcccggat
    10921 ttgctgcagc ctgccttcca tgaccgttat gtgcagtacg ccccctgaaa ggtcctgctc
    10981 tgtcagaaag gcccggctgg tgatatatcc ccggcgtata tagccgtccg tcacggcatc
    11041 cgtgaccgcg gtcagccccg tgatattcag acactgattc acccacggtg ccaccagtct
    11101 gtcggtttct gcagacgtca gtcgggtggc cccactgaca acaatgcgtg acaccgtaaa
    11161 gcagggacct tcagtaccgg cagacatttc cggagacggt atgacagtca gcggcgcact
    11221 gcgctccagc gcatcacgct gacgctggtt ttcatccagc aactgctgct gttgctgctg
    11281 aattgcactg cggtcacccg gagacagcat ggctgccgga gacgaaaatg cggggataaa
    11341 aaaaccagaa aaaacggaga gggagagata acatatcccc gaagaggcgc gggcgcgccg
    11401 gaatatatca taacagggga cagacacagc ccggaataaa agagacgttt tcgtcagaat
    11461 gttgttctgc cggtgctgca tgttctcccc gtattctggc ctgtgtaatg tcagtgtaaa
    11521 ttcatgtcaa aaatgtgtca gcgggaaatt tacacctgaa aaagtctgtc atgaaacagc
    11581 tttatgacaa gatatcaaaa aaaatgtcaa aaacgtgatc tttcttcagc ttttcagaat
    11641 attgagagcc tagaggtcaa tataaattct gggacacata tgcatttacc attaaaaccg
    11701 gcacaacagg ctgttcagtc tcaatccatg ctttctgttt tatcgatctc agaatatcgg
    11761 cagacaaata aaaaaggaat gtcgagagag ctaactctag atcactctcc acctcacaag
    11821 ccatttacaa aaatgataca ggcatatctt tggtccctgc cacaaccaga cattgacaat
    11881 ccaatataac cccaaatata tttcggatac gaagcttggg atttatacag acatttacta
    11941 tttaattata aaatttgtat tacatacata agaggcacta atgaaacgtt atataaaatg
    12001 gcttgccatc acaattttta tcagtatgtt gagtgcctgt gcccgtacgg ctccagtgca
    12061 acagataagc accactgtca gtgtgggtca tactcaggag caggttaaaa atgccatttt
    12121 gaaagcaggt gcacagcgca agtggattat gacgcaagtg tcccctggag ttattaaagc
    12181 tcgctatcaa acacgaaatc acgttgctga ggttcgcatt acatatacag ctacttacta
    12241 taacatcaaa tatgacaata gcctgaatct gcaggcttct gatggaaaaa ttcataaaaa
    12301 ctataaccgt tgggtgcgta acctggataa agatatacag gttaacttgt ctacaggagc
    12361 aacgttatag tgatgctgat atcgggctgg aatagcccga tatcattaaa cttctctata
    12421 agaatggtta tatatgtacg aacaggacac actcagcaca ctggatgcca tcacggaggc
    12481 acaacggatt gcttttgctc ctatgctttt ccaggcagct ttgtgcctgc gcaatgcagg
    12541 aatattagat tatttggatc aacagggtaa acagggagct ccccttaacg cgattactga
    12601 acagacagtc ctgaatgaat atgccgtgag tgtgttactg gatatggggt taagcgggcg
    12661 aattattacc tgtaaagagg gaaattatta cctggcgaaa atcggtcatt acctcctgca
    12721 tgacactatg actcgcgtga atatggattt cacccaggat gtctgttacc aggggctttt
    12781 cttccttgcc gattcactca atgaaggaaa accttccggg ttaaaagtat ttggcgactg
    12841 gccgacgata tatccggcac tttcgcaatt accagatgct gctcgtgcta gctggtttgc
    12901 atttgatcat tactattcag atggcgcatt taacgccgca ctgccttatg tattcgccaa
    12961 caaccccaca accttgtacg atgtgggcgg caatacgggt aagtgggctt tgcgctgctg
    13021 caaatacaat gaaaacatcg ccgttacgtt attagatctg ccacaacaaa tcgtactggc
    13081 gaaagaaaat atcgcgaatg caggtttttc tgatcgcata gatttccaag cggtggatat
    13141 gctaagcgat gccccgttac caggcgaggc tgatatctgg tggatgagcc aatttttaga
    13201 ctgtttttca cccgaacaaa taattagcat actcagcaaa atcgccagcg taatgaagcc
    13261 tggcgcaaaa gtttgcatca tggagttgtt ctgggatgcg cagcggtttg aagcagcatc
    13321 gtttagcctg aacgcgtcct cactttattt tacctgcatg gcaaacggta acagccgttt
    13381 ctatagtgca gagaaatttt acgactatct gaataaagca ggattccagg tagcagagcg
    13441 ccacgataat ttaggcgttg gacatacact tttgatatgc caaaagaaat aatcgtcact
    13501 taataatttt ggtttaccgc gggcacgcgt tgatgaaatt tgcattaaat attaccaact
    13561 ggcaggcgct ggcgcctgga cttagcgatg tgcagcaatg gcaagcatgg tcgcgccagc
    13621 cgtgggcgat tgaccctgcc gcgccgctgg caaaattaag tgaactgcca atgatgactg
    13681 cccgtcgtct cagttcaggc agcaaactgg ctgttgaatg cggtctggca atgctacgcc
    13741 gctatcagcc tgacgccgtt ttgtatacca gtcgtcatgg cgaactggag cgtaattacc
    13801 gcattgttca tgcactggca accgaacagg cgctttctcc gacggacttc gcgctttccg
    13861 tacacaattc ttccgtgggt aacctgacca ttgcggccaa acagccgatc gtttcgtcat
    13921 cgctttcagc tggtcgcgac agtttccagc aaggtttgtg tgaagtgctt agcctgctac
    13981 aagcgggata ccagcgcgtg ttgatggtcg attttgacgg ttttctgccc gagttttatc
    14041 acccacaact gcctgcagaa atgccgacct ggccgtacgc cgtcgcgctg atgattgaat
    14101 ctggcgatga ctggcaatgt gaaacgcagt cagccattgc ggtaaatgaa acaacactgc
    14161 cgcaaagcat gttgttttta cagcactatt tacaaaatgc aggcgcattt tcactgtctg
    14221 gcgagcgcgt gcaatggcgc tggagccgca gatgaataag ctgatgtcgc gcctgggatg
    14281 ggcatggcga ctggtcatga ccgggttatg ttttgccctg tttggtctcg gcggcctgtt
    14341 gctttcagtc gtatggttta acatattgtt agttttggta tgggatactt cccgccgccg
    14401 tcgtttggcg cgtcgcagca ttgccgccag ttttcgcctg tttttaactg tcgcgaaagg
    14461 gcttggcgtg ctggattatc gtattgatgg cgctgaaatt ttacgtcagg aacgcggttg
    14521 cctcgtcgta gccaatcatc ccacgctgat cgattacgtg ctgctggcgt cagtcatgcc
    14581 agaaactgac tgcctggtga aaagcgcgct gttaaaaaat ccttttcttg gcggcgtcgt
    14641 gcgggcggca gattatttaa tcaacagcga agctgaaacc ctgttacccc gctgccaaca
    14701 gcgactggca cagggtgaca ctatcctgat tttccccgaa ggcacacgaa cgaaacccgg
    14761 tgaaaaaatg acattacagc gcggtgcagc gaatattgcc gtgcgctgcg ccagcgattt
    14821 acgcatcgtg acaatcggct gtagtgagca tctgctggat aaacaaagta aatggtatga
    14881 cgttccgcct gccaggccgt tttttacggt tgaagtccat gggcgggtag aaatcaataa
    14941 attttacgat gcaacttcac atgagccggc gctggctgca aggcagttaa accgacatct
    15001 tttgcttcaa ttacaacaag gttgtttacc tctctcagga ataaataatg caagcgcttt
    15061 atctggaaat taaaaatctc atcatcagca ccctcaatct ggacgagttg actcctgatg
    15121 atattgatac caatgcgcca ctgtttggcg acggtttagg gctggactct atcgatgcac
    15181 tggaactggg gctggcagta aaaaatgagt acggcgtcgt gctctctgcg gaaagcgaag
    15241 aaatgcgtca gcactttttc tccgtcgcca ctctggcctc tttcattgct gcgcaacgtg
    15301 cctgaggaaa gccatcatga cagaccaaca aaccatttat caggaagtct cagccctgct
    15361 ggttaagctg tttgaaatcg atccgcagga catcaaacct gaagcgcgcc tgtacgaaga
    15421 tctggaactg gacagcatcg acgccgttga catgattgtg cacctgcaaa agaaaaccgg
    15481 taagaaaatc aagccggaag agtttaaagc agtgcgtaca gtccaggatg ttgtagaggc
    15541 tgtaaaacgc ctgctacaag aaggctaatt tgatgtcggg cattcgctcg ctaccgatga
    15601 ttaagttgct gaccggatta ttgctgctcg cctggccttt tttaatctgg ctcggtttgg
    15661 cgcataacag cctgcactgg ctgtcgccgc tgatggcgtt gctgctttta ttgcgcctgc
    15721 gccagccccg tcgtcaggca gggccgctac aagccgtcac aaaaatcgtc gctgttgtgg
    15781 gcattgtatt gtgtgtctcc agttttctgt tgaaaacgca ccagttactg ctgttttacc
    15841 cggtagtggt taacgctgtc atgctggcgg tttttggcgg ttcgctgcgg tcgacaatgc
    15901 cgatagttga gcggctggca cgtttgcagg aaccagacct cccggaaaag gccgtgcgat
    15961 atacccgccg cgtaacgcag gtctggtgcc tgttttttat tttcaacggc agtattgcgt
    16021 tatttaccac cttgtatggc aacatgcctt tgtggacagc ctggaacggt atcattgctt
    16081 accttctgat aggtttcctg actgcagggg aatggctgat acgttgtcag atgatcaaac
    16141 ggaaaacacc atgaatcaga ctctctcact ttcacagtgg ttgacagcct ctcgccctgt
    16201 aacaactcct gttgcctggc ttggggaata tacctggaca ctggggcatc tgcggcatga
    16261 tgtagcgctt ttgatcgacc atttacgcga ccagccaggc aatcgctggg cactttgttt
    16321 tgaaaacagc tatcttttta ttgtagcgtt gttggccacg ctacactcag gtaaaatccc
    16381 agtaatcccg ggccataatc gcgccgcttt gttaaatgta caacgctcct tgtttgattg
    16441 catcctgagt gataaaaggc tgggctggaa cggtccacag tttgttgtaa gcacatctca
    16501 ccagatgacg accagcacga tagcctttga tgatattgct tctgacgcgt ttattgaact
    16561 tttcacatcg ggttcaaccg gacaaccaaa acaaattgca aaacctgtta tcagccttga
    16621 tcaagaagca aacatgctgg caactcactt tgcagaccga ctactgggct gtcgttttgt
    16681 agcatccgtg atgcctcagc acctgtatgg gctgactttc cgtatttttt tacctatggc
    16741 acttggcctg ccattgcatg ccgcaatgct ctggtattct gagcaacttg ccgcattaag
    16801 ccatacctac cgctatgcct ttgtcagcag cccggcgttc cttaaacgac tcgaccttca
    16861 tctgacgcct ccccccgtgg aaatgattct ctctgccggc ggtatgctac catggcagga
    16921 tgtagagaaa acttccacct ggctgaatat ctggcccgat gaaatctatg gtagtaccga
    16981 aacaggtatt ctcgcatggc gttaccgtca acaggataat atcccctggt ttactttttc
    17041 tgatgttcat ctttcacagg aaagtgaggg ggttcgtgtc ttttcgccac tcatccctgc
    17101 tgaaggattg gtgcttgacg atatgttgca attcaatgaa aacggtcagt tccacctgat
    17161 cggacgacgt ggtcgagtgg taaaaataga ggagaagcgg atatcactgg ttgaaatcga
    17221 acaacggctt ctggcacttg acggtattat ggatgtcgct gcaataccat taatacgcaa
    17281 tggccgccag gctatcggtg tcgtggttgt accaaataaa gaggcccgtc aaatatggca
    17341 atgctccgga gggaaaactc tggagttgtc ctggcgcaaa gcactattac catggcttga
    17401 acctgttgca gtacctcgtt actggcgcat tattgatgaa ataccagtaa acaatatgaa
    17461 taagcgtgtc tatgcacaat tacaggagtt atttcatgat acgccatgaa attgagcgcc
    17521 atcaggttca actgcaggag atggaaatcg ttctatacct tgacccgatg ctctactggt
    17581 ttaatggtca ttttgccgta cagcctctgt taccaggcgt ggcccagttg gactgggtaa
    17641 tgcattatgc taccacgctg cttgctccgg gctggcgttt tcgtagtatc cagaatgtta
    17701 aatttctggc gccacttata cctgagacga ctgttaccct gcaattaaca tggcaggaaa
    17761 caagtcaggt actcactttc tgctatcagc gtcacgacgg tgacgcccgc cacaccgcca
    17821 gtagcgggaa gatccgccta tgtcggtaaa cttttctccc tgcgtgttga tcccctgcta
    17881 caaccacggt gcgatgatgc cgggcgtgct ggcgcgtctt aagccattta atcttccctg
    17941 tattgtggtg gatgacggca gcgatgccgc cacgcaacag caactggata atctggttgc
    18001 cgaacagcct ggcgtgacct taattcgcct ggcagaaaac acaggcaaag gcgcggcggt
    18061 aatgcgcggc ttacaggcgg cggcagacgc gggcttcagc catgcggtgc aggtggatgc
    18121 tgacggtcaa catgcgattg aagatatccc caaactgctg gcactcgctg aacaacaccc
    18181 tgcggcgctg atctccggcc agccaattta cgatgactcc atcccccgct cacggcttta
    18241 cgggcgctgg gtcacccacg tctgggtatg gatcgaaacg ctctccctgc aactgaaaga
    18301 cagtatgtgc ggctttcgcg tttatccggt tgcgccaacg ctgcaactgg caaaatacgc
    18361 caccatcggc aagcggatgg atttcgacac tgaagtgatg gtgcgcctct actggcaggg
    18421 aaacacctgt tatttcgtgc caacccgcgt cacttatccg caggacgggc tttcgcattt
    18481 tgatgccctg aaagataacg tccgcatctc gctcatgcac acgcgtctgt ttttcggcat
    18541 gttgccgcgt attccttcac tactgatgcg ccgctcttcc agccactggg cgcggcagag
    18601 tgaagtgaaa ggattatggg gaatgcgcct gatgctgctg gtctggcgtc tgctgggaag
    18661 aacggcgttt agcgcgctgc tttacccggt ggtgggcgtc tactggctca ctgctgctcg
    18721 tgcgcgcaaa gcgtcgcaag actggctcgc ccgtgtacga cagcatcaac cacaggcggc
    18781 aaaactcaac agctatcagc actttctacg tttcggtaat gccatgctcg acaaaatcgc
    18841 cagctggcgc ggcgagctac aactggggca cgatgtgctg tttgcgccag gcgcagaagc
    18901 ggcgcttgac gtccgcgatc cgcgcggcaa attgctgctg gcctcgcatc ttggcgatgt
    18961 ggaagtgtgc cgggcgctgg caaaaattca gggctacaaa accattaacg cgctggtgtt
    19021 tagcgaaaac gcccaacgct ttaaacagat aatgcaggag atggctcccc aggcaggcat
    19081 taacctgatg ccggtaacag atatcggccc ggaaaccgcc atcctgctga aagagaagct
    19141 ggataacggc gaatgggtgg cgattgtcgg tgaccgcatc gccgtcaccc cgcaacgcgg
    19201 cggcgactgg cgcgtctgct ggagtccgtt tatgggccag cctgcgcctt tcccacaggg
    19261 gccgtttatt ctcgcgtcta ttttgcgctg cccggtgaat ctgattttcg ccctgcgcca
    19321 gcgcggcaag ctgcatattc actgcgaaac ctttgccgac ccgctgctgt tgccgcgtgg
    19381 cgaacgccaa caggggctgc aaaacgctat cgaccactac gccgcgcgtc tggaacatta
    19441 cgcgctccag tcgcccctcg actggtttaa ttttttcgat ttctggcaac tgccggaaat
    19501 tcaggacaag gagtaaaggg tgcttaacga tccccgattt accgctgaag tcgagctgac
    19561 cattcccttt cacgacgtcg atatgatggg cgtggcctgg cacggtaact attttcgcta
    19621 ctttgaagtg gcccgcgagg cgctgctcaa ccagttcaat tacggctatc ggcagatgaa
    19681 agagtcaggt tatttgtggc cagtggtcga tgcgcgggtg aaatatcgcc acgccctcac
    19741 ctttgagcaa cgcatccgcg tgcgtgcgca tatcgaagag ttcgaaaacc gtctgcggat
    19801 tggctatcag attttcgatg ccgaaacggg caaacgcgcc accacaggat acaccattca
    19861 ggtcgcagta gaggagcaaa gccgtgaact gtgctttgtc agccccgata ttctgtttga
    19921 acgcatggga gtcaaaccat gaaattttta ccgctgctgg cgctgctgat tagcccgttt
    19981 gtgagcgccc tgaccctgga cgatcttcag caacgcttta ccgaacaacc ggtgatccgc
    20041 gcccatttcg atcaaacccg gacgattaaa gatctgccgc agccgctgcg atctcagggt
    20101 cagatgttga tcgcccgcga ccaggggctg ttgtgggatc aaacttcacc attccccatg
    20161 cagctattgc tcgacgataa acgcatggtg caggtgatca acggccagcc gccgcaaatc
    20221 atcacggcag aaaataaccc gcagatgttc cagtttaacc acctgctgcg cgcgctgttc
    20281 caggccgatc gcaaagtgct ggaacaaaac ttccgcgtcg aatttgccga taaaggcgaa
    20341 ggccgctgga cgctgcgcct gacgccgacc accacgccgc tggataaaat tttcaacacc
    20401 atcgatctcg ccgggcaaac ctatctggag agcattcagc ttaatgataa acagggcgat
    20461 cgcaccgata tcgctcttac ccaacatcaa ctgacgccag cgcaactgag cgatgacgaa
    20521 cgccaacgtt ttgccgccca gtaaacgccc cgcgctgtta tgggggctag tctgcctggt
    20581 catggcggtg gtattgctga tcctgctgcc gcaatcacgg ctgaacagta gcgtgctggc
    20641 tatgttaccc aaacaggcga tgggcgatat tcccccagcg ctgaatgacg gctttatgca
    20701 gcgtctggac cgccaactgg tgtggctggt cagccccggt aaagaggcta atcccagggt
    20761 ggctcaggaa tggctgacgc tgctgcaaaa gtccgctgcg cttggcgaca ttaaagggcc
    20821 aatggatgcc gccagccagc aggcgtgggg ggcgtttttc tggcagcacc gcaacggcct
    20881 gattgacccc gacacccgcg cccgcctgca aaacggcggc gaagcgcagg cacaatggat
    20941 cctctcccag ctttattctg cgttctccgg ggtaagcggc aaggagttgc aaaacgatcc
    21001 gctgatgtta atgcgcggct cgcagctggc gatggcgaaa aacggccagc gtttacgact
    21061 gatggacggc tggctggtga cgcaggatcc ccagggcaac tactggtatc tgctgcacgg
    21121 cgaactggcg ggatcgtcgt ttgatatgca gcaaacccac cagctcatca cgaccctgaa
    21181 tacgctggaa aaagatctga aaacgcgtta cccgcaggcg cagttgctct cgcgcggcac
    21241 ggtgttttac agcgattacg ccagccaaca ggcgaagcag gatatctcca ccctgggcgt
    21301 ggcaacgctg ctgggggtga tattgatgat tgtggcggtg ttccgctctt tacgcccgtt
    21361 gctgctttgc gtgatttcca tcggcatcgg cgcgctggcg ggaacggccg ccacattgtt
    21421 gattttcggc gaattacacc tgatgacgct ggtgatgagc atgagcgtta tcggcatttc
    21481 cgctgactac acgctctatt acctcaccga acggatggtg cacggcaacg acgtttcgcc
    21541 gtggcaaagc ctggcgaaag tgcgcaatgc cttgctactg gcgctgctca ccaccgtggc
    21601 ggcatatctg attatgatgc tcgccccttt tcccggtatt cggcaaatgg cgatttttgc
    21661 cgccgtcggg ttgagcgcct cctgtctgac cgtcctgttc tggcatccgt ggctgtgccg
    21721 tggcctgccg gtacgcccgg ttccggcgat ggcgctgatg ctgcgctggc tggcggcgtg
    21781 gcggcgtaat aaaaaactgt cgctgggtct gcccgtcgcg ctggcgctgt tttcgctggc
    21841 ggggatgtca atgctacgcg tcgatgacga tatctcgcag ttacaggcgc tgccgcagca
    21901 tattctggcg caggagaaag ccattaccgc cctgaccgga caaagcgtcg atcaaaaatg
    21961 gtttgtagtt tacggcgact ccccacagca aaccttggga cggctggaga catttaccac
    22021 ctcgctggag aaagcgaaaa aagacggcct tatcagcaac taccgcacca ttccgctgaa
    22081 ctccctggcg cggcaggagg aagatttaga cctgctgaaa acggctgccc cgacggtaac
    22141 aaaagcactg caaaatgccg ggctgacggc ggtgaacccg gatcttaacg ccatgccagt
    22201 gaaggttgat gaatggctgg caagccccgc cagtgaaggc tggcgtctgc tgtggctgac
    22261 gctggaaaac ggcgaaagcg gcgtactggt gccggttgaa ggggttaaaa gtagcgcgtt
    22321 gcagcaggaa atcgccagct attaccctgg cggcattgcc tgggttgatc gcaaaagcac
    22381 ctttgatgaa ttgttcgcac tttaccgtta tgtgttaacc ggcttgttgc tggtggcgct
    22441 ggcagtaatt gcctgcggcg cagtggcccg cctcggctgg cgcaaagggc ttatcagcct
    22501 ggtgccttcg gtgctttcgc tgggctgcgg tctggcagtg ctggcgatga gcgggcaggc
    22561 ggtgaatctc ttttcgctac tggcgctggt gctggtgctt ggcatcggca tcaattacac
    22621 gctgtttttc agtaatccgc gcggtacacc gttaacttcc ctactggcga tcacgctggc
    22681 aatgctcacc accttgctga cgctgggaat gctggtattc agcgccaccc aggccatcag
    22741 cagttttggt attgtgctgg tgagcggtat tttcaccgcc ttcctgcttt cgccgctggc
    22801 tatgcccgat aaaaagagaa caaaaaaatg atcaaatcca ccttctggcg agcgctcgcc
    22861 ctgaccgcta cgcttatcct caccggctgt agccactcgc aaccggaaca ggaaggccgc
    22921 ccgcaggcgt ggctgcaacc tggcacacgc atcacgctgc ctgcgccggg gatttcaccc
    22981 gccgtcaatt cccagcaact cttgagcggc agcttcaacg gcaaaaccca gtcgctgcta
    23041 gtgatgctta acgccgacga ccagaaaatc acccttgccg ggctgtcgtc ggtcggcatt
    23101 cgcctgtttc tggtgaccta cgatgcaaaa ggtctacgcg ccgagcaatc catcgtcgtc
    23161 ccgcagctgc cgcccgcaag tcaggtgctg gctgacgtaa tgctcagcca ctggccgatt
    23221 agcgcctggc aaccgcaact tcccactggc tggacgcttc gcgacaacgg caacaaacgc
    23281 gagctgcgta acgccagcgg caagctggtc acggaaatca tctatctgaa tcgccagggc
    23341 aaacgcgtgc caatcagcat tgagcagcat gtctttaaat accacatcac cattcaatac
    23401 ttaggtgact gatatgattt atatttccgc cgtcggcatg atcaacgccc tgggaaataa
    23461 tcttgacgaa atcgcagcca acctggctcg cggtgtcgcc cccggcatgc gtccgcgcgc
    23521 aggctggttg caggggcatc cgcaggcggt cctggctggc gtggatggcg agcttccgct
    23581 tatccccgaa aaattcgctg cgcaccgctc gcgcaacaat cagatcctgc tggcggcgct
    23641 ggcgcagatc cagccgcagg ttgatgacgc tatcgcgaaa tatggccgcg agcgcatcgc
    23701 gattgtgctt ggcaccagca cctccggcct gcatgagggc gacacgcacg ttaacctgcg
    23761 caccaacgga cagccgagca ccacatggca ctacgcgcaa caggaactcg gcgatccgtc
    23821 gcgttttctc agccactggc tggcgctcga cggcccggcc tatacccttt caaccgcctg
    23881 ctcttccagc gccagagcga tgatcagcgg gcgcaggctt atcgaagcgg ggctggtcga
    23941 tgccgccatt gtggggggcg cagatactct gagccggatg ccgattaacg gttttcacag
    24001 cctcgaatcg ctctcgccaa ccttatgtca gccgtttggt cgtgaccgcg caggtatcac
    24061 cattggcgaa ggggcgggac tgatgttatt aacccgtgaa ccgcagccca tcgcgttact
    24121 gggcgttggt gaatcgagcg acgcttacca tatttcggcc ccgcatccgc aaggcgaagg
    24181 ggcgatccgc gccattaacc aggcgctgac cgatgcgcag cttacgccag atgacatcgg
    24241 ctatatcaac ctgcacggta ccgccacaca actcaacgac cagattgaat caatggtggt
    24301 taacgccctg tttggcaaac gcataccgtg cagctccacc aaacatttga ccggacacac
    24361 gctcggcgcg gctggcatta ccgaggcggc aattagtatg ttgattttac agcgtgattt
    24421 accgctgccc gctcaggact tcagcctgtc gccgcgcgat cccacgctgc caccgtgtgg
    24481 cattatcgaa aaaccgcagc cgctggcacg tccggtgatc ctgtccaatt ccttcgcctt
    24541 tggcggcaat aacgccagca ttctgctcgg gagggtttcg tgagccacta tttatccccc
    24601 ggcgcttatc tgccccacga cgcgcccatg ttactgctgg aagacgtggt gagcgtcagc
    24661 gatgacagcg cggtgtgccg cgtgacggtt tcgcccagtg gcgtactggc accgtttctc
    24721 gacccggacg gcaatctccc tggctggttt gcccttgaac tgatggcgca aaccgtcggc
    24781 gtctggtctg gctggcatcg ccaccagcag ggcaaaaaca gtattgagtt agggatgata
    24841 ctgggcgcgc gtgaattgct ttgcaccact ggcattctgc ccgcaggcca gacgctgacc
    24901 attaccgtca aactgctgat gcaggacgat cgcttcggca gctttgagtg cagcatcaac
    24961 gacggcgagg cgacgggccg cattaacacc ttccagccga ccgcggaaga acttaccacc
    25021 ctgtttcaac aaggagcgtc ctgatgagtc gttcagttct ggttactggt gccagcaaag
    25081 gcattggtcg cgccatcgcc tgcaagcttg cggcagacgg ctttaacatt ggcgttcact
    25141 atcaccgtga cgccacaggc gctcaggaaa cgctgaatgc cattgtcgcc aacgggggta
    25201 acgggcgttt gctcagtttc gacgtcgcaa accgcgaaca gtgtcgggaa gtgctggagc
    25261 atgagatcgc gcaacacggt gcctggtacg gcgtggtcag taacgccggg atcgcccgcg
    25321 atgccgcttt tccggcttta agcgatgacg actgggatgc ggtgatccac accaacctcg
    25381 acagtttcta caacgtcatt cagccgtgca ttatgccaat gatcggcgcg cgccagggtg
    25441 ggcgcatcat cacgctgtcc tcggtttccg gcgtaatggg caatcgcggc caggtgaatt
    25501 acagcgcagc caaagccgga attatcggcg caacaaaagc actggctatc gaactcgcaa
    25561 agcgcaaaat caccgtcaat tgcatcgcgc cagggcttat tgataccggg atgatcgaaa
    25621 tggaagaaag cgctctgaaa gaggcaatgt cgatgatccc catgaaacgt atgggccagg
    25681 cggaagaagt tgccgggctt gccagttatt taatgtctga tattgcgggt tacgtcaccc
    25741 gccaggttat ttccatcaac ggagggatgt tatgacacgt cgcgtagtga ttacaggaat
    25801 gggcggcgtg actgcctttg gtgaaaactg gcaggacgtt tctgcacgct tactggcgta
    25861 tgaaaatgcg gtgcgcaaaa tgccggagtg gcaggtctac gatgggctgc acaccctgct
    25921 tggcgcgcct gtggatgatt tcacgctacc ggagcactac acccgcaagc gtatccgtgc
    25981 gatgggccgc gtgtcgcaaa tgtcgacccg tgccagcgag ctggcgttag agcaggcagg
    26041 gttgattggc gatccgatat taaccagcgg cgaaacgggc attgcgtacg gttcatcgac
    26101 cggcagtacc ggtccggtga gcgaatttgc caccatgctg actgaaaagc acacgaacaa
    26161 catcaccggc accacctatg tgcagatgat gccgcacacc accgctgtta atactggcct
    26221 ctttttcggc ctgcgcgggc gggtgatccc aacctccagc gcctgtacct ccggcagcca
    26281 ggcgatcggt tatgcgtggg aagccattcg ccacggttat caaacggtga tggttgccgg
    26341 tggtgccgaa gagttatgcc cttcagaagc agcggtgttt gatacccttt ttgccaccag
    26401 ccagcacaat gatgcgccga aaaccacacc ttcaccgttc gatgaaaacc gcgacggact
    26461 ggtgattggt gaaggcgcag gtacgctcat tctcgaagaa ctggagcatg ccaaagcgcg
    26521 cagcgcgacc atttacggtg aaatcgtcgg ctttgcgact aactgcgacg cggcacatat
    26581 tacccagccg cagcgcgaaa ccatgcagta ttgtatggag caatcgctaa aaatcgcagg
    26641 gttaagcgca caagatatcg gctatatttc agcgcatggt acggcgaccg atcgcggtga
    26701 tatggcagaa agtctggcga cagcaaccat ttacggtgat aatgtgccgc tctcctcgct
    26761 gaaaagttat tttggtcata cgcttggtgc ctgtggcgcg ctcgaagcct ggatgagtct
    26821 gcaaatgatg cgcgaaggtt ggtttgcgcc tacgctaaac ctgaataaac cagacccgaa
    26881 ttgtggcgcg ctggattaca tcatgcatga agcccgcaag gttgattgtg agtttttgca
    26941 gagtaacaac tttgcctttg gcggcattaa tacatccatc attattaagc gctggccctg
    27001 agttatacaa ccacatggac atcccatgtg gtgattcttc aaaaaatctg aatcaatgaa
    27061 tatgaacctc aaattctctt acgcatactg aagagcattc cgtataacga ttcagcagac
    27121 aaaactttat cagtccacac gaatcaacgg atgcacagat gacacgcccg gttatcaccg
    27181 ggcaatggtt gccataacgg gtaacataag ggactgaaca gttgtcttta gcgcaacgaa
    27241 ctgatctaac gtacctgaac ggacaacctg aaaacagcct tccatcctgc attgtccgcc
    27301 agtgcttatt accaaccacg tctgttgcag taaagtaatc agttgcgaac attatttatc
    27361 aatcgtgacg ctggctcgcg catggcagaa aaggtcctga ctgagcaaca acaggcatcg
    27421 tactgttgct gctccatgtt gtttatatga acgcatcccg tttgcaaggc ttccgtgttt
    27481 ttgacgttga cggtagattg ctgctctata tccttattgc agccggtgct gcgggcctca
    27541 atgagattcg ctgactctcg ccttattcac gcaacgagct ttaagctcag aaaaacatcc
    27601 aagtttggtg agtcacagtc ttacctgtcg ttgtcatcaa tataggaatg gataatctgg
    27661 agagcgatac tgcaggctga tattgcggtt ttgcgacaag tttttagcat ccgcttctac
    27721 gccaaatacc tgaagtagga atggatgcgt tagcagcata catccggaac tgcgcgggga
    27781 atagccggaa acggctatcc ctaccacaat agctaacttt gtaatagcgc tcttttaggc
    27841 tcatgtactc tgacgtaatt ctatctggta gttgaattgc acgatacgcg actcttctga
    27901 ttcatcatga ttattattca atagtccaat cagtaactta cctgtctcta tccccatttt
    27961 tttgtagtca atacggatgg atgtcaaagt tgggtgtgtt tgatcgcaat tgtctgaacc
    28021 gtcaagacaa gctacagcga tatttccagg gatttttaac aaacgccgct ggcactcaaa
    28081 cattataccc agcgcaatct cttcgtggct gcaaatcaca gcatccagct ctggttgtcg
    28141 cagtaatatc tcgtttaatg cgtaacgccc caattgcaga cttgcggcat ccggcatggt
    28201 gataacaaga tcagtgttct ggtaatggct cagcattgct ttatgccagc cgttcagttg
    28261 ctgacgttgc aggcggttgt ccatatgcgc accgataaat ccgatattct tataaccctg
    28321 ctccagcaga tgttcggtca gtttatgtga tgcttcaaaa aatgctgttt cgatggtaat
    28381 ttgagccgga ctgaaagggg aaccgataac gttaatggta ggtatatttg ttttctcgag
    28441 tatctggtga gttttatcag ccaactgaga accaaaaata actaacgccg ccgggttgcc
    28501 ttgaagaagc gtcatcagaa tttcagcttc tttgagctga ttatactcat gacacccaag
    28561 cagtagctgg aattcatttt tattaagcac ctgctgtaat gactgcataa agcgggagct
    28621 ggctttatct gtcagggaag gaaccagtac ggcgactgta tgactgtgac cagatgcaag
    28681 ggcgccagca gttctgttgg gaatataacc caactcttct acggcctgtt ctatcttttc
    28741 cctcagcttg tcagaaacct gttccggtgt tctcagcgcg cgggaaacgg tcatggttcc
    28801 gacacctgca aagttagcga cctcctgcag cgtcacccgt
//
LOCUS       DAZGHF010000055        28760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGHF010000055 DAZGHF010000000
VERSION     DAZGHF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.055"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(31..1155)
                     /locus_tag="ACHK3A_002470"
     CDS             complement(31..1155)
                     /locus_tag="ACHK3A_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO8740533.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTWASEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGACLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
     gene            1288..2841
                     /locus_tag="ACHK3A_002471"
     CDS             1288..2841
                     /locus_tag="ACHK3A_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO8740534.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3223..4113
                     /gene="metF"
                     /locus_tag="ACHK3A_002472"
     CDS             3223..4113
                     /gene="metF"
                     /locus_tag="ACHK3A_002472"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO8740535.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            4442..6622
                     /gene="katG"
                     /locus_tag="ACHK3A_002473"
     CDS             4442..6622
                     /gene="katG"
                     /locus_tag="ACHK3A_002473"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO8740536.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            6716..7621
                     /gene="yijE"
                     /locus_tag="ACHK3A_002474"
     CDS             6716..7621
                     /gene="yijE"
                     /locus_tag="ACHK3A_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO8740537.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(7648..8265)
                     /locus_tag="ACHK3A_002475"
     CDS             complement(7648..8265)
                     /locus_tag="ACHK3A_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO8740538.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(8541..9644)
                     /gene="gldA"
                     /locus_tag="ACHK3A_002476"
     CDS             complement(8541..9644)
                     /gene="gldA"
                     /locus_tag="ACHK3A_002476"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO8740539.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(9655..10317)
                     /gene="fsa"
                     /locus_tag="ACHK3A_002477"
     CDS             complement(9655..10317)
                     /gene="fsa"
                     /locus_tag="ACHK3A_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO8740540.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(10329..12830)
                     /gene="ptsP"
                     /locus_tag="ACHK3A_002478"
     CDS             complement(10329..12830)
                     /gene="ptsP"
                     /locus_tag="ACHK3A_002478"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO8740541.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVMKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIPLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLAAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13139..14218
                     /locus_tag="ACHK3A_002479"
     CDS             13139..14218
                     /locus_tag="ACHK3A_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO8740542.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            14233..14553
                     /locus_tag="ACHK3A_002480"
     CDS             14233..14553
                     /locus_tag="ACHK3A_002480"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO8740543.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            14604..16901
                     /locus_tag="ACHK3A_002481"
     CDS             14604..16901
                     /locus_tag="ACHK3A_002481"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO8740544.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGS
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRSGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            16867..17745
                     /locus_tag="ACHK3A_002482"
     CDS             16867..17745
                     /locus_tag="ACHK3A_002482"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO8740545.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSQENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            17747..18088
                     /locus_tag="ACHK3A_002483"
     CDS             17747..18088
                     /locus_tag="ACHK3A_002483"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO8740546.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFDHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18075..18926)
                     /locus_tag="ACHK3A_002484"
     CDS             complement(18075..18926)
                     /locus_tag="ACHK3A_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8740547.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGATGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(19152..20885)
                     /locus_tag="ACHK3A_002485"
     CDS             complement(19152..20885)
                     /locus_tag="ACHK3A_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO8740548.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(21068..23719)
                     /gene="ppc"
                     /locus_tag="ACHK3A_002486"
     CDS             complement(21068..23719)
                     /gene="ppc"
                     /locus_tag="ACHK3A_002486"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO8740549.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(24021..25172)
                     /gene="argE"
                     /locus_tag="ACHK3A_002487"
     CDS             complement(24021..25172)
                     /gene="argE"
                     /locus_tag="ACHK3A_002487"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO8740550.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            25326..26330
                     /gene="argC"
                     /locus_tag="ACHK3A_002488"
     CDS             25326..26330
                     /gene="argC"
                     /locus_tag="ACHK3A_002488"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO8740551.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            26338..27114
                     /gene="argB"
                     /locus_tag="ACHK3A_002489"
     CDS             26338..27114
                     /gene="argB"
                     /locus_tag="ACHK3A_002489"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO8740552.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            27175..28548
                     /gene="argH"
                     /locus_tag="ACHK3A_002490"
     CDS             27175..28548
                     /gene="argH"
                     /locus_tag="ACHK3A_002490"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO8740553.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVESKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         6662 a         7225 c         7888 g         6985 t
ORIGIN      
        1 taatttatgg tcgtttgtcg agtgtattga ctaacaagat ggagtgagcg attcattgta
       61 ttttaacgcc agtgaggtca gcatttgtgt tacgcgacct gcggataata acgcgctttg
      121 ggtaaagtcg tgttggtctg tcagccatgt gataaaacaa cgactggttg ccagagaaac
      181 atttgcgccc ctttctaagc aggctcccgt gagtaggcag accatcttgc agttattccc
      241 ctgctcccag cgctgccact gattttgcca ccaaccatgg tatgtcgttc ccttaaaatg
      301 ccagggcgta tcagcgttac tgtcactgat ttcttcttct acgaaccaaa gttcaccttg
      361 ttgttggcgg aactgatctg cccaggctgt aataattgcg cctcttagaa aacattcgtg
      421 tgaaataata tgcgttttga catctggata gcggattttc agtattctga aatgcccgtg
      481 tgccgtgata accgggcgta gtatggctcc cttaccgttg cgcttgagta cagcactgcc
      541 atactgaatt tcatcgaact ggctccggcg caagaattgc gcttcacgca tatctacctt
      601 ctgcacgata tcgcgatggt gcatagttgg ttctgtgttg cccttgtaac tgtaccgcag
      661 actgtctcct gtctgatgtt gcgtataatt cgtggagaga tgaagaatgt tgccacgagt
      721 ggcgtcaaga ctgactaaaa catacagttt ttgtactgcg ctggcccctt gaaagggtat
      781 gaacagttga acggtttcaa tggcctgggg atgcgtaatt tctttctgat actgtttagg
      841 cgtccagact tttttgcagt tacggcattg aatgcgttgc gaaccctgtg gattgtgacc
      901 ataacaaatc atgtctgtgc cataacatac cgggcagaaa tgacccttct ccgaggccca
      961 cgtagataag tgaactgaca accagtcacg aaattgttgc tcgtcaaaca acggcgggta
     1021 actgccacat gcccgacagt gcagtgccgg ataacccagt ttatagtctg gccaactgta
     1081 atcttccgat gaggccaatc ccaaatttcg gcaaccgaac gttttacagg cattaatcgt
     1141 aaacagagtc gacatatatc accaacgatt tcacaaggtc gttgattttg tccggcaact
     1201 atcccctgca gatgtgatta ctctcacata aataagtctt tcagaacgta atgtagcaat
     1261 cctttatctc actgaaaggg attgtttatg aaaattaaaa tacttgctgc gggtatcgca
     1321 ttaacactgc cgttctgggc ttgcgctaaa gatgtcacta ttatttatac caacgacctc
     1381 catgcgcatg tagaacctta taaagtgccg tggatcgctg acggtaaacg ggatattggc
     1441 ggctgggcaa atatcactac gctggttaag caagaaaaag caaaaaataa agcgacatgg
     1501 ttttttgatg cgggcgacta ttttaccggg ccatatatca gcagcctgac caaaggtaag
     1561 gctattatcg atattatgaa tactatgccg tttgatgccg tcacgatagg caaccatgaa
     1621 tttgaccacg gctgggacaa cacattatta cagttgagtc aggcaaaatt cccgattgtg
     1681 cagggcaatg ttttttatca gaacagcagt aaatcattct gggataagcc ctataccatc
     1741 atcgaaaaag acggtgtgaa aataggcgtt ataggtttgc acggtgtatt tgcctttaat
     1801 gacacagtat ctgctgccac gcgagtgggt attgaggcgc gtgatgaaat taaatggcta
     1861 caacgttata tcgatgaact taaaggcaag gttgatttga ccgtcgccct gatccatgaa
     1921 ggtgttccgg cccgccagtc cagtatggga ggcacagatg tgcgtcgcgc gctggataaa
     1981 gatattcaga cggcaagtca ggtgaaaggg ctggatattt tgatcaccgg acatgcacat
     2041 gtgggtacgc cggaaccgat taaagtcggc aatacgctca tcctctccac tgacagcggc
     2101 gggattgatg tgggcaaact ggttctcgac tacaaagaga agccgcataa ttttacggtg
     2161 aaaaacttcg agcttaaaac catttacgct gacgagtgga agcccgatcc acaaacgaaa
     2221 caggtgatcg acggctggaa taaaaagctt gatgaagtgg tgcaacaaac ggtggcgcaa
     2281 tcgccggttg aattaaaacg tgcctatggt gaatcggctt ccctcgggaa cctggcggct
     2341 gacgctttac tggtagcggc gggtaaaaac acccaattgg cgttaaccaa ctctggcggc
     2401 attcgcaatg agatccccgc aggggcgatc acgatgggcg gcgtcatcag tacgttcccg
     2461 ttccccaacg aactggtgac gatggaactc acgggtaaac aattacgcag tctgatggaa
     2521 cacggcgcaa gtttaagcaa tggcgtttta caggtatcga aaggcctgga aatgaagtac
     2581 gacagcagca agccggttgg tcagcgggta atcacactga ctctgaatgg caaacccatt
     2641 gaagatgcga cggtttatca cattgctact cagagtttcc ttgctgatgg tggagatggt
     2701 tttaccgcct ttaccgaagg gaaagcgcgt aacacaacgg gcggttatta cgtttatcac
     2761 gcggtggttg attacttcaa agcgggcaac accattacgg atgaacagct caacggtatg
     2821 cgcgtgaagg atatcaaata aaagtgcgca ctgaattgac tcaactgttt gttacccgtt
     2881 gattgcgcag atcctgtatt cggcctacgc gagggcgcag gccgaatcag atgccttagc
     2941 atcaccatcc ggtggcagga cgttgttttt cgaattagcc caggtgtcgc tgtgttgtca
     3001 gaatatgagt caaaacgtgc gaggtggatt gtgctataga gaaatctttt atcgcctcta
     3061 attaagatga gtttttttca tcttccctga tttttcctca ccatcattgg ttatttttcg
     3121 gttgacgccc ttcggctttt ccttcatctt tacatctgga cgtctaaacg gatagatgtg
     3181 cacaacacaa catataacga taagcgattg atgaggtaag gtatgagctt ttttcacgcc
     3241 agccagcggg atgccctgaa tcagagtctg gcagaagtcc aggggcagat taacgtttcg
     3301 ttcgaatttt tcccgccgcg taccagtgaa atggagcaga ccctgtggaa ctccatcgat
     3361 cgccttagca gcctgaaacc gaagtttgta tcggtgacct atggcgcaaa ctccggcgag
     3421 cgcgaccgta cgcacagcat tattaaaggc attaaagatc gcaccggcct ggaagcggca
     3481 ccgcatctta cctgtattga tgcgacgcct gccgagctgc ggaccattgc acgcgactac
     3541 tggaataacg gtattcgcca tatcgtggcg ctgcgtggcg acctgccgcc gggaagtggt
     3601 aagccagaaa tgtatgcttc tgacctggtg acactgttaa aagaagtggc agatttcgat
     3661 atctccgtgg cggcgtatcc ggaagtacat ccagaagcga aaagtgccca ggccgattta
     3721 ctcaacctga gacgaaaagt ggatgccgga gccaaccgcg ccattactca gttcttcttc
     3781 gatgtcgaaa gctacctgcg ttttcgcgat cgctgcgtct cggcaggcat tgatgtggaa
     3841 atcattccgg gaattttgcc ggtttcgaac tttaaacagg cgaagaaatt tgccgacatg
     3901 accaacgtgc gtattccggc gtggatggca caaatgttcg acggcctgga tgacgatgct
     3961 gaaacccgca agctggtggg cgcgaatatt gccatggata tggtgaagat tttaagccgt
     4021 gaaggagtga aagatttcca cttctatacg cttaaccgcg ctgaaatgag ttacgcgatt
     4081 tgccatacac tgggtgttcg acctggttta taaatagtgt gccttttgtg aaaattacac
     4141 agtgatcaca aattttaaac agagcacaaa atgctgcctc gaaatgaggg cgggaaaata
     4201 aggttatcag ccttgttttc tccctcatta cttgaaggat acaaatctaa aacccttttt
     4261 tataaagcat ttgtcctaaa tcggacataa tcaaaaagtc ttaattacga tcaatttgat
     4321 ctacatctct ttaaccaaca atatgtaaga tctcaactat cgcatccgtg gattaattca
     4381 attataactt ctctctaacg ctgtgtatcg taacggtaac actgtagagg ggagcacatt
     4441 gatgagcacg tcagacgata tccataacac gacagccacc ggcaaatgcc cgttccatca
     4501 gggcggtcac gaccagagcg caggtggggg cacaaccacg cgcgactggt ggccaaatca
     4561 actccgtgtt gacctgttaa accaacattc taatcgttct aacccgctgg gtgaggactt
     4621 tgactaccgc aaagaattca gcaaattaga ttactacgga ctgaaaaaag atctgaaagc
     4681 cctgctgaca gaatctcaac cgtggtggcc tgccgactgg ggcagctacg ccggtctgtt
     4741 tattcgtatg gcctggcacg gtgcgggtac ttaccgttca atcgatgggc gcggcggcgc
     4801 gggtcgtggt cagcaacgtt ttgcaccgct gaactcctgg ccggataacg taagcctcga
     4861 taaagcgcgc cgcctgttgt ggccaatcaa acagaaatat ggtcagaaaa tctcctgggc
     4921 cgacctgttt atcctcgcgg gtaacgtggc gctggaaaac tccggcttcc gtaccttcgg
     4981 ttttggtgcc ggtcgtgaag acgtctggga accggatctg gatgttaact ggggtgatga
     5041 aaaagcctgg ctgacccacc gtcatccgga agcactggcg aaagcaccgc tgggcgcaac
     5101 cgagatgggc ctgatttacg ttaacccgga aggcccggat cacagcggcg aaccgctttc
     5161 tgcggcagca gctatccgcg cgaccttcgg caacatgggc atgaacgacg aagaaaccgt
     5221 ggcgctgatt gcgggtggtc atacgctggg taaaactcac ggtgccggtc cgacatcaaa
     5281 tgtaggtcct gatccagaag ctgcaccgat tgaagaacaa ggtttaggct gggcgagcac
     5341 ttacggcagc ggcgttggcg cagatgccat tacctctggt ctggaagtag tctggaccca
     5401 gacgccgacc caatggagca actatttctt cgagaacctg ttcaaatatg agtgggtaca
     5461 aactcgcagc ccggctggcg caatccagtt cgaagcggta gacgcaccgg aaattatccc
     5521 ggatccgttt gatccgtcga agaaacgtaa accgacgatg ctggtgaccg acctgacgct
     5581 gcgttttgat cctgagttcg agaagatctc tcgtcgtttc ctcaacgatc cgcaggcgtt
     5641 caacgaagcc tttgctcgtg cttggttcaa actgacgcac agggatatgg ggccgaaatc
     5701 tcgttacatc gggccggaag taccgaaaga agatctgatc tggcaagatc cgctgccgca
     5761 gccgatctac aacccgaccg agcaggacat tatcgatctg aaattcgcga ttgcggattc
     5821 tggtctgtct gttagtgagc tggtatcggt ggcctgggcg tctgcttcta ccttccgtgg
     5881 tggcgacaaa cgtggtggtg ccaacggtgc gcgtctggca ttaatgccgc agcgtgactg
     5941 ggatgtgaac gccgcagccg ttcgtgctct gccagttctg gagaaaattc agaaagagtc
     6001 tggcaaagct tcgctggcgg atatcatcgt gctggcaggt gtggttggcg ttgagaaagc
     6061 cgcaagcgcc gcaggtttga gcattcatgt accgtttgcg ccgggccgcg ttgatgcgcg
     6121 tcaggatcag actgacattg agatgttcga actgctggag ccaattgctg acggtttccg
     6181 taactatcgc gctcgtctgg acgtttccac caccgagtca ctgttgattg ataaagcaca
     6241 gcaactgacg ctgaccgcgc cggaaatgac tgcgctggtg ggcgggatgc gcgtactggg
     6301 tgccaacttc gatggcagca aaaacggcgt cttcactgac cgcgttggcg tattgagcaa
     6361 tgacttcttc gtgaacttgc tggatatgcg ttacgagtgg aaagcgaccg acgaatcgaa
     6421 agagctgttc gaaggccgtg accgcgaaac tggcgaagtg aaatacacag ccagccgtgc
     6481 ggatctggtc tttggttcta actctgtcct gcgtgcggtg gcggaagtct acgccagcag
     6541 cgatgcccac gagaagtttg ttaaagactt cgtggcagcg tgggtgaaag tgatgaacct
     6601 cgaccgtttc gacctgctgt aatctgacct tcttcagcga ctgcctttca tgcagtcgct
     6661 gaagtttctt taccggcgta tagtgtccac aggaaaacta cacactggat ctctcatgtc
     6721 tgccgcagga aagagcaacc cactggcaat cagtggcctg gttgtgctca cacttatctg
     6781 gagttatagc tggattttca tgaagcaagt caccagctac atcggtgcct tcgactttac
     6841 cgccttacgc tgcattttcg gcgctctcgt tttattcatc gtccttttat tacgtggtcg
     6901 cggaatgcgc ccgacaccgt ttaaatacac cttagccatt gccctgttac aaacctgcgg
     6961 gatggtcggc ctggcgcagt gggcgctggt cagcggagga gcggggaagg tggcaatcct
     7021 gagctatacc atgccgttct gggtggtaat tttcgccgcg ttgtttctcg gtgaacgcct
     7081 gcgacgcgga caatatttcg cgattctgat tgccgctttc ggcttatttt tggtgttgca
     7141 gccgtggcaa ctcgatttct cttcgatgaa aagcgccatg ctggcaatcc tctccggtgt
     7201 gagttggggg gcgagcgcga ttgttgctaa acgtctgtat gcccgccatc cgcgcgtgga
     7261 tttattgtcg ctaacatcct ggcagatgct gtatgcggcg ctggtgatga gcgtggtcgc
     7321 tttactggtg ccgcaacgtg aaattgactg gcaacccacc gtgttctggg cgctggccta
     7381 cagtgcgatt ctggcgacag cgctggcgtg gagcttatgg ttgtttgtat tgaaaaactt
     7441 gcctgccagt attgccagct taagcacact ggccgttccc gtttgcggcg tactcttttc
     7501 ctggtggctg ctcggtgaga atccgggggc agttgaaggt agcggtattg tgctgattgt
     7561 actggcactg gcgctggtga gccgtaagaa aaaagaagcc gtcagtgtaa aaaggatctg
     7621 aattttttct tcatgtgggg agatctctta tttagcaaaa taccgatagt gccccaccat
     7681 ccgccagcta aacagtacat cttcttcctg cgcgcctgcg ccaatgttat gtatcaccag
     7741 cggcgtaccg tcgcgggcga agccatctga caccacccca atatgtgcca gcccgttatc
     7801 taatcgccag gagacaatat cgcccgcttg atagtcactg gggttcttgc tggtggggcg
     7861 tgttttatcg tggcgggtaa accaggtttc cagattaggc acccggcggt gatcgatgtt
     7921 gctgtccggg cgctttaact gccatttttg cgggtacgca gcaaaattct tcgccatatc
     7981 ttcgtgcacc agtttctgca aatcgacctt ctggctgcgc aatgcacgga tcaccacatc
     8041 ggaacatacg ccgcgttctt gcgggacatc accgccagga taggtaagct gcacatacgc
     8101 cggatcgtaa aatagcgtgc tgccgatttg ctgtctggcg ccgtctgcga tggcaagatt
     8161 ggtgttggcc tggatttgta ccacggttgg tggaacggcg ggagatttta aggagtaact
     8221 ggtaaatacc gtcagaagac tgaataacgc cagtgaagct ttcatctgtt tcgtccgtga
     8281 aaataagagt cacagattcg accttccggg ctaacgattg ttctgtggag aaaatgtcca
     8341 gaagcggata aaaaaacacc cgaacaaggt ggaagtttca gattactggc acagcgtgcc
     8401 ggatgcggcg tgaacgcctt atccggccta caaaagtacg taaatttact atattgcaga
     8461 gattgcgtag gcctgataag cgtagcgcat caggcaattt tgcgttcaaa ctcccggaca
     8521 agccgggagt ttggagtagg ttattcccac tcttgcagga aacgctgacc atactggtcg
     8581 gctaccagca gtgcggcgta aacctgatct ggcgtcgcgc cgccaggcat gttgtggatg
     8641 gtttcacctt ctgcacatgc cgcttctgcc acaattcgca ttttcgccgg gacatcttct
     8701 ttaatatcca gttgagcgag agttattggc aaacctaccg catggctaag tgcagctacg
     8761 gtttcgattt cctccaccgg cgcgttttcc agaaccagct gcgtcagcgt accaaatgcc
     8821 actttttcac cgtggtaata gtgatgcgcg tccgggatag cggtcaggcc gttatgcacc
     8881 gcgtgagccg ccgccagccc gccgctttca aaaccaacgc cgctcaaata ggtgttcgct
     8941 tcaatcacgc gctccagcgc cggggttact acatgctgct cggcggcaag catcgctttt
     9001 tcgccttctt ccagcagggt gttgtagcac agttcagcca gtgccagcgc cgcctgggtg
     9061 cacttgccgc ccgccatggt ggtcgcgccg ctacgagagc aagcacgcgc ttcaaaccag
     9121 gttgccagcg catcgccgat acccgccgct aacagacgtg caggtgcgcc agcgacgatt
     9181 ttggtgtcga caatgaccat attcgggtta tttggcaaca tcagatagcg gtcaaattca
     9241 ccatcatcgg tgtagataac agacaatgcg ctgcacggtg cgtcggtaga ggcgatagtc
     9301 ggtgcgatcg ctaccggaac acccatgaaa tgtgccagtg ctttggcagt atcgagagtt
     9361 tttccgccac cgataccgag aattgcgcca cactgcgcag tctccgcgat gccacgcaga
     9421 cggtcgatct cattttgcga acattcaccg ccaaacggcg caatttctac taccagtcca
     9481 gcatctttaa agcttttctc gacagtggat tgagcaaaac ctaaaacaaa tttgtcaccc
     9541 accactaacc agcgttctgc cagcggcttc aggtattcgc ccagacgatt aatcacatca
     9601 gcgccctgga tgtatttacc cggtgattga ataatgcggt ccataattgc tcctttagag
     9661 atgagtagtg ccaaatgcgg cattccagtc gtgttcgaac ttctctatag ctgactctac
     9721 cgcaggggtg ttgagcattt gttgcgctac atctaagggc agggtgatgg attcacatcc
     9781 tgccagtaaa cagtccagcg cctgacgtgg tgttttaaag ctggcagcca gcaccatgct
     9841 ttctggcgca tgcatttcca acagcgcttg cagctcctgt acagtacgaa tgccgtcacc
     9901 gccctgggca tctacgcggt taacgtatgg agcaacgtat ttggctccag ccagcgccgc
     9961 cagtaatcct tgcgctgcgc tgtacactgc ggttcccagc gtagtaatgc cttctttctt
    10021 cagcatttta attgctgcca gaccttcaga ggttaccgga attttaacca caatgcccgg
    10081 aatagcgtcg cgcaggcgtt tcgcttcttt caccataccc tgcgcgtcgc ggctcatggt
    10141 ctgagcaaac agaattccct catcaccgat cgctttttga aggcgcggca gcacttccca
    10201 gatggactcc ttgctggcag cgataatgct cgggttagtt gtcaccccgg caatcgggaa
    10261 tatgcgtgcc agacgttcga cttctgcgac gttagcggtg tccagataca gttccatgat
    10321 gtttcctttt acagttccag ttcatgttgc agcaggctgg cgatagcgtc ggcagaggcg
    10381 gcgttaacca gcgcgttacg gaattcttcg tgcatgatgc gacgagcgag gcgcgagaaa
    10441 atgcgcatgt gctgatcgcc cgcagcatgt ttgttcaggg ttaacataat gatgaattgc
    10501 gcttcatcat cgccccagcg caccggcgct gccagacgcg ccacgctgat ggtggattgc
    10561 tcaatgtgtt ctgatttgct gtggggaatg gcaaaactaa agcccagacc ggtagagaaa
    10621 acggcctcgc gcgcccacaa gtcggcttcc agtttacgcg gatagcgaca gcggcccgcc
    10681 agcagcaggt tatcggtcat gcctttgagc acttcttctt tgctgcgcca gtcgctttcc
    10741 agtgtgatgc actcggcggt gaccagcggt gcgtcttgtt gggtcatgcg gaattgcgcc
    10801 agcaggtgtt ccacttccag cgaagtacgg caggccattg cctggttgag caacttgcgg
    10861 cactcacggc tatcaagttg cgccatccgc gctttcgccg ccggaattga tggtgcgctc
    10921 atgctgagtt catccagccc taaaccgacc agcaacggca gcacggaacc tttcgctccc
    10981 agttcaccgc acagaccaat ccatttgccc tggcgatgca ccgcctgcac ggcgtaatcg
    11041 agcgcccgca agaatgctgg attcaggctg ttgtagtgac gagtcacctt agcgttatcg
    11101 cgatccactg ccagtagata ttgcgtcagg tcattactac caatactaaa gaaatcaatc
    11161 tcttcgcagc attgatcgat gatgaacatc accgacggca cttccagcat gatgccgagt
    11221 ggaatcttct catcaaacgg aatgtgttcg ttacgcagtt gctgtttggc ttccgccagt
    11281 ttttctttca cccataagat ctcttccatt gaggagatca tcgggatcat gattttcagg
    11341 ctgccgtgag cagaggcgcg caggatcgac cgtagttgtg tggtaaacaa cgacgcgtat
    11401 tcttcataaa tacgcaccgc gcgataaccg aggaacgggt ttgcctctgc gggaatattc
    11461 agataatcaa cgggtttgtc accgccaata tccatagtgc gcacaataat gctgcgtccg
    11521 ttggcggact ccagcgcctg acaaaaaatg ttgtacagct cgctttcgcc cggtgcactg
    11581 gtgcgatcca tatagagcat ttcagtgcgg aataaaccaa cgccttccgc accattgccg
    11641 aatgcggcct gcgcttccac ggagtgagcg atgttagcgg caatttcaat gcggataccg
    11701 tcagcggtac gggcttgttg ggtcagccag acacgctgtt gctcacgcag ggcgtcctgt
    11761 acgcgggctt cttgctgata ataacgggcc acggcttccc ctggctcaac cacaatcgcc
    11821 cctgcgttgc cgtcgatata aatcgtttga tgctgccacg gagtaagggc atcaatatcc
    11881 acacctacca gcgtcggaat gttgaacgaa cgggcaagga tcaccgtatg tgaggtggtg
    11941 ccgccacttt tcagtaatag acctttgagg tgatttttat ccagttcgag gaactggctg
    12001 ggggtcagtt catcagccat acaaatggcg ggctgcgtca gtttgcccgg tgccgggaag
    12061 cgttgctcac cgtagatttg ctggagtaac tggaagcaga cgtcgcgtac gtccagggca
    12121 cgttcttgca ggtagctgct gctggaacgg gcgaactctt cgcaaaagtg attcgcactg
    12181 gcaacaatcg cttcggcgca gcttaatccg gcgctgacac ctgccagtaa atgttcgcgc
    12241 agggaagtat cgccagccag ggatcggtga gcttccagta tcgcgctggt agcaccgtcg
    12301 ctatccagca gacgaaattc aatgtttttc atcaccagtg tcaggccgtt ttccagcgcg
    12361 gattgctcgg cgtcaacgcc tttggcagca ggaagattac ccagcgcatt gagatctaaa
    12421 gaagagatcg gcgtcagaat gccgcctgca ctaccactgc acacggtgcg agcacggata
    12481 atttgcggat tcagattggt cagtgaaacc ggcagtggtt ccagttcgtc agattttact
    12541 tccgccagcg gtgcgtcgca gtgggggaat tcatcgcgca gccattggct taaacgctgg
    12601 tgcgcttcct gttcgtcggc cccggaaatc agtaactggc agttatcgcc caccagcgta
    12661 tcggtgccaa tcagcgcaag ggcgcttttg gcgttgccct tgcggtcagt gcgcaggtta
    12721 tgccactcaa tttgtgatga aaaagtatta cacagcgttt caacgtggct tgccggacgt
    12781 gcatgtacgc cgttaggtag ctcacaaata aattccacaa tcagggccat tgcctctctc
    12841 ccggtacggt tttctgttta caggataaga gcgcacggca acggcctgcc atgtgacaaa
    12901 tctgccaaaa gctggacaaa tgtaatgtaa ccgtcaattt gcgacgcgtc tcacaagacg
    12961 ccgttttgcg gcatgcttcc ggatcatcgc aagttatgag gcggatcgca tttttgtact
    13021 gatattacaa aagtccagta aatggccttt ttatccactg tttgctccgc ctgcgattgc
    13081 ctattgtttc ctccatcaaa aatatggaca tgggcttttg caagcccggg agcagcttat
    13141 gaatgagttg gtgcagatcc tgaaaaatac ccgtcagcat ttaatgacgg gcgtttcaca
    13201 catgattccc ttcgtggtat cgggcggtat tttgctggcg gtttccgtca tgttgtatgg
    13261 caaaggcgca gtgccggatg ccgtagccga tccgaatctg aaaaaactgt ttgatatcgg
    13321 cgttgcgggt ttgacgctga tggtgccttt cctcgccgct tacattggtt actccattgc
    13381 agagcgttct gcgctggctc cgtgcgctat cggtgcctgg gttggtaaca gctttggtgc
    13441 gggcttcttt ggtgcactga tcgccgggat tatcggcggc atcgtggtgc attacctgaa
    13501 gaaaattccg gtgcataaag ttctgcgctc ggtgatgcca atcttcatca ttccgatcgt
    13561 cggcacactg attaccgcag gcatcatgat gtgggggctg ggcgagcctg tgggggcatt
    13621 gaccaacagc ctgactcagt ggcttcaggg gatgcagcag ggcagcattg ttatgctggc
    13681 ggtgatcatg ggcctgatgc tggcgttcga tatgggcggt ccggttaaca aagtggccta
    13741 tgccttcatg ctgatttgcg ttgctcaggg tgtttatacc gtggtggcca ttgctgccgt
    13801 gggtatttgt attccgccgc tggggatggg gctggcgacg ctgattggtc gtaaaaattt
    13861 ctccgcagaa gagcgcgaaa ccggtaaagc ggcgctggtg atggggtgcg ttggggttac
    13921 tgaaggggcg attcctttcg ccgctgccga tccgctgcgt gttattcctt ccatcatggt
    13981 cggttcagtt tgtggtgcag taactgcggc gctggtcggt gcgcagtgct atgcaggctg
    14041 gggcggtctg attgtgctgc cggtggttga aggcaagctg ggttatatcg cagcagtagc
    14101 tgtcggagca gtggtgacgg ctgtttgtgt gaacgtgctg aaaagtctgg cgcgtaaaaa
    14161 tgggtcttcg actgatgaaa aagaagacga cctggatttg gattttgaaa ttaattaatt
    14221 gaggaacgag ccatgacgaa aattattgca gtaaccgcgt gtccatctgg tgttgcccat
    14281 acctatatgg cggcagaggc gctggaaagt gccgcgaaag cgaaaggctg ggaagtgaaa
    14341 gtagaaaccc agggctcgat tggtctggaa aacgaactga ctgcggaaga cgtggcgagc
    14401 gccgatatgg tgatcctgac caaagatatc ggcatcaagt ttgaagagcg ttttgctggc
    14461 aagaccatcg tgcgcgtcaa catcagcgac gcggtaaaac gcgctgatgc cattatgagc
    14521 aaaattgaag ctcacctggc acaaaccgct taagttctta tcccgcccgt aagggcgggt
    14581 tcgctttccc acaggagttc ctcatgacga atcgtatctc tcgcctcaaa actgcactgt
    14641 ttgccaatac ccgtgaaatc tcgctggagc gtgcgctgct ttataccgcc agccatcggc
    14701 aaaccgaagg cgaaccggtg atattgcgcc gggcgaaagc aacagcgtat atccttgaac
    14761 atgttgaaat ttcgattcgt gatgaagaac tgattgccgg taaccgcacc gtaaaaccgc
    14821 gcgccgggat tatgtcgccg gaaatggacc cttactggct gctgaaagag ctggatcaat
    14881 tcccgacgcg tccgcaggac cgctttgcta tcagcgaaga agataaacgt atctaccgtg
    14941 aagagttgtt cccgtactgg gaaaaacgtt cgatgaaaga tttcattaac gggcagatga
    15001 cggatgaagt aaaagccgcg accagcacgc agattttcag catcaaccag acggataaag
    15061 gccaggggca cattattatt gattacccgc gcctgctgaa tcacgggctg ggtgagctgg
    15121 tagcgcagat gcagcaacat tgtcagcaac agccggagaa tcacttttat caggcagcgc
    15181 tgttactgct cgaagcctcg cagaaacaca ttttgcgtta cgccgaactg gcggaaacga
    15241 tggcggcaag ctgcactgat gcccagcgtc gcgaagaact gctgactatt gcggagatct
    15301 cccgccataa cgccgaacat aagccgcaga cgttctggca ggcgtgccag ttattctggt
    15361 acatgaacat cattctgcaa tacgaatcca acgccagttc actatcgttg gggcgcttcg
    15421 accagtatat gttgccgttc tatcaggcat cattaaccca gggcgaagat ccggcgttcc
    15481 tgaaagaact gctcgaatct ttatgggtga aatgcaacga catcgtgctg ttgcgctcca
    15541 ccagtagcgc gcgttatttc gccggtttcc cgaccggcta taccgcactg ctcggcgggt
    15601 taaccgagaa cggacgtagc gcggtgaacg tgctttcgtt cctttgcctt gacgcttatc
    15661 aaagcgtgca attaccgcaa ccgaacctcg gcgtgcgcac taacgccttg atcgacacgc
    15721 cgttcctgat gaaaaccgca gaaaccattc gcctcggcac cggtattccg caaatcttta
    15781 acgatgaagt ggtggtgcca gcgttcctca accgtggcgt ttcgctggaa gatgcacgcg
    15841 actattccgt agtgggctgt gtggaattat ctattcccgg cagaacctac ggcttgcatg
    15901 acatcgcgat gtttaatctg ctgaaagtga tggaaatctg cctgcatgaa aatgaaggca
    15961 atgctgcgct gacttatgaa ggtttactgg agcagatccg cgccaagatc agccactaca
    16021 tcaccctgat ggttgagggc agcaatattt gcgatatcgg tcaccgtgac tgggcacctg
    16081 taccgctgct ctcatcgttt atcagcgatt gtctggaaaa aggcagcgat attaccgatg
    16141 gcggcgcgcg ttataacttc tccggcgtac aggggatcgg tatcgccaac ctgagcgatt
    16201 ctctccatgc gttgaaaggg atggtttttg agcaacagcg tttaagtttt gacgaattgc
    16261 tgtcggtatt aaaagccaac ttcgcaacgc cagaaggcga aaaagtccgt gctcgcttaa
    16321 ttaaccgctt cgagaaatac ggtaacgata tcgacgaggt ggataacatc agcgccgaac
    16381 tgttacgcca ctactgcaaa gaagtggaaa aataccagaa cccgcgcagc ggctacttca
    16441 cgccgggatc gtataccgtt tctgctcacg tcccgttggg atcggtggtt ggcgcgacgc
    16501 cagacggtcg ttttgccggg gaacaactgg cagacggcgg cttgtcaccc atgctgggcc
    16561 aggacgcaca agggccaacg gcggtactga agtcagtcag taagctcgat aatacgctgc
    16621 tgtctaacgg tacgttgctg aacgtgaaat tcactccggc gactctggaa ggtgaagcgg
    16681 gattacgcaa actggccgac ttcttacggg cgtttaccca gcttaagttg cagcatattc
    16741 agtttaacgt ggtgaacgcc gacacgttgc gggaagcgca acagcgccca caagattatg
    16801 ccgggctggt ggtgcgcgtt gccggataca gcgccttctt tgtcgaattg tcgaaggaga
    16861 tccaggatga catcatccgc cggacagcgc atcagctgta acgttgtgga aacgcgccgc
    16921 aatgatgtgg cgcgcatttt caacattcag cgttattcac tgaatgacgg cgagggcatt
    16981 cgtacggtgg tcttttttaa aggctgtccg catctttgcc catggtgtgc taatccggag
    17041 tcgatctccg gcaaaatcca gacggtacgc agagaggcga aatgtctgca ctgtgcgaaa
    17101 tgtttgcgtg atgcggatga atgcccctcc ggggcgtttg aacggattgg ccgcgatatc
    17161 agccttgacg cgctggagcg ggaagtgatg aaagatgaca ttttctttcg cacgtccggc
    17221 ggcggcgtca cgctttctgg cggcgaagtg ttaatgcagg cggagtttgc tacccgtttt
    17281 ttacagcggc tgcggttgtg gggcgtgtca tgcgccattg aaactgccgg agacgcgcca
    17341 gccagcaagc tgttaccgct ggcgaaattg tgcgatgaag tgttgttcga tttaaaaatt
    17401 atggacgcga ctcaggcgcg ggatgtggtg aagatgaatc tgccacgcgt gctggagaat
    17461 ctgcgtttgc tggtgagtga gggcgtcaac gtgatcccgc gtttaccgct gatccctggt
    17521 ttcacgctca gccaggagaa tatgcagcag gctctggacg tgctgatccc gctgaatatc
    17581 aggcagatcc atctgttacc gtttcatcag tacggcgaac cgaaataccg cctgctgggg
    17641 aaaacatggt cgatgaaaga ggtgcctgcg ccgtcgtcag ctgatgtggc aacgatgcgc
    17701 gaaatggcag aacgggccgg atttcaggtt accgtgggag gttaaaatgg catacctggt
    17761 ggcagtaacc gcctgcgtca gtggcgtggc gcatacttat atggcggcgg aacggctgga
    17821 aaagttgtgc ctgttagaga agtggggcgt cagcattgaa actcagggcg cgctgggaac
    17881 ggagaatcgt ttagcggacg aggatatccg ccgggcggat gttgcgctgt taattacgga
    17941 tatcgagctt gccggtgctg agcgatttga ccattgccgc tatgtgcaat gcagcatcta
    18001 cgctttcctt cgtgagccgc agcgggtgat gagcgcggta cgcaaagtgc tttctgcgcc
    18061 gcagcaaacc catctcattc tggagtagtc ggtttttctg tcagttggct gtggtactgc
    18121 cgacgatatt ccgacggtga gcgttcggta tttttacgaa acagacggca gaagtagttg
    18181 ctgtcgacaa agccgcaggc gtgcgccact tcttttacct tcaggtcata gcctttcaat
    18241 aacgtcttag cgtgctccag tcgcgtgtga ttcaggtatt cgttaaaacc agtggccccc
    18301 gttttttgaa acaggtgcga gaggtaattt ggcgaaatat agaatgcctg tgcgacagat
    18361 tcgcgggtaa gcgcggaggc atagcgttcg tcgatataat cacgaatagc ttcgaacagt
    18421 gcctggctgc gtgaggcggt ctggatttgg ctgccaagca aatcgcggca gtggctaagc
    18481 aggctggcga cgataagcct tgccgtttgc tgctcctgcg actgcatttg catttcattg
    18541 agcgtttgta gcagaaaaga acctatgcgt gggccgcgtc gggcgacgtg ttgcttcgcc
    18601 agattttgat attgtttgcc atcccattgc acgacgctga agccgagttg ctgtttgcca
    18661 aacagcacgc taaaggtagt ggcgggggct tgccattgtg gaaaattcca gccaccagcg
    18721 ggaacgtaca gcacatcgcc gggaactaac gctgctccag cgccggtatc aataaactca
    18781 ccttccagca caatttccag ccgtggaaaa tcgacctgat acgcaagatc gggcaccggg
    18841 ccgttgctgc tggcaaagta aatctggcgc agggacagcg ggccgttgat caggcgggag
    18901 agcagatagc tgacgtcgtg atacatgtta ttcccttagg tatgtagacg ggaaaacagt
    18961 aactggcatt atgaaaaaag gccacctgaa aagtgtggcc tgatgggagt gagatgtaaa
    19021 cattgcggca gtttcagtcg acttgtattg ccggatgcgg cgtaaacgcc ttatccggcc
    19081 taccgccgcg atctgtaggc cggataagac gcgtcaagcg tcgcatccgg caaataacat
    19141 ctggctgata attactgatt acccacctga tcgccatacg gcagagtgtc gtaatcgatc
    19201 agtgcgtttt tcttatacgg gttaccaatc cagcgggtag tttctttgaa ctgcggattc
    19261 accactgaac gggttggatc gtaaccgtcg taagataagc ccgccaaatc tgaccaggtg
    19321 tggatcagtt ccgccaggct gtatttacga tcaacatcct gtgagaaatc acgcggatga
    19381 gtcgcttgcc atttttctga cgtccacagc aggaacggaa tggtgtacat atgacgcgtc
    19441 gggttgtctt cattacgccc ctgagttttg tgcggcggcg tgtcgtaaac ctcttcaccg
    19501 tggtcagaga aataaaccag gaaaccgttc ggatcggtcg ctttaaagtc tttaatcagg
    19561 ctggcaacca cgtgatcgtt gtacaggtta gcattgtcat aatcattata tgactccagc
    19621 tcttctgcgt ttaatcccgg tggaacatgc tctgtattac cgtcaaattt gccctgatct
    19681 tccggataac ggtacttgta cttgatatgc gtacccagca gatgaacgat gatcagtttc
    19741 ttcggcgcag ggtccttcag cacttcctgg aacggcttca gcacgttggt gtcgtattca
    19801 cgcgcactct gcgtacgttg ctggttcatg tagtactgct tatcggtctg gcgcgaaaat
    19861 accgtcagca tggtattgcg ggcggtcatc gtctgctggt tggtgatcca gaaggtttta
    19921 taacccgcct gtttcatcat gttcatcagc gacggctgcg tcagatacag atccgggttc
    19981 ttttcattgg caaaggtcag cgcctgttgc aggatttcaa tggtgtacgg acgagacgta
    20041 accacgttat taaacacggt cagattcgga tcggttttat gcagcgcatc cagctccggc
    20101 gtggtttcac gcggataacc gtacagactc atgcgtccac gctgggtcga ttcgccaatt
    20161 accagcacta aggtgcgcgg ttcgttaccc gattcatctt tgaaattcgc cagcggcggc
    20221 aaggcattat tttcattcag tagctttgtt agcgagttta gttgctgacg atactgataa
    20281 tagccggtca ggaattgcca cggtgcggca ggctccatac gcgatgccag gttatccaac
    20341 gttttctcaa atggcttgtt tttgataaac gtattcatgg cgatcggatg cagaatcaag
    20401 ccataaagta gggcaaaaga gacaacataa cgccacggct ttggaatata gaccgggcgc
    20461 aggcgcgtcc acagcaaaac tgccaccgcc gtataggcca gcgcgataag cacaatcttc
    20521 aggctgaaat actggcttaa atactcgctg gcttcgttgg tgttggtttc aaacatcaca
    20581 aacagaacgc tctgcgagaa ctcctgaccg tagatgacgt agtagcacaa cgctgccaga
    20641 gaggccgccc atagcaccac gccgatcact gcggcaataa ttttaatccg cttcggaaag
    20701 aggaataccg ggatcaacca cagcgaactg aataacagcg agtcgcgaat gccgttagtg
    20761 ccactataac cactgatgta aataatggcc tgtagcagag tagagaaaaa ccaaaagtag
    20821 agcagtgccc aacccagggc cttccagcta aaaaggggtt tagcctggac ttctgtggaa
    20881 tgcatagtaa gaacctgtct tgaaaaaaat atcgccgaat gtaacgacaa ttccttaagg
    20941 atatctgaag ggatatccag attttgaata aaatgcagac agaaatatat tgaaaacgag
    21001 ggtgttagaa cagaagtatt tcagaaaacc ctcgcgcaaa agcacgaggg tttgcaggag
    21061 aggaagatta gccggtatta cgcatacctg ccgcaatccc ggcaatagtg accattaacg
    21121 cctgctcgac gcgaggatcc ggctcatggc cttctttttc tgcctggcgg gagcggtgca
    21181 gcaactcggc ctgcaatacg ttcagcgggt cggtgtaaat attccgtagc tgaatagact
    21241 ctgcaatcca cggcagatcg gccatcaggt gagaatcgtt ggcaatcgcc agcaccactt
    21301 tgatgtcttc ttcttgcaga ttgcgtaact ctttacctaa cggccacagt gctttgtcta
    21361 ccaggcgttg gtcatagtat tccgccagcc acaggtctgc tttggcgaag accatctcca
    21421 gcatgccgag acgcgtcgag aagaatggcc aatcgcggca catggcttcc agctcgctct
    21481 gtttgccatc ttcgaccact ttttgcagcg ccgtacctgc acccagccag gcggggagca
    21541 tcaggcggtt ttgtgtccag gcaaaaatcc acggaatagc gcgcagtgac tcaacgccac
    21601 cggtcgggcg acgttttgcc ggacgtgaac ccagcggtag tttgccgagt tcctgctccg
    21661 gcgtggcaga gcggaagtaa ggcacaaaat ctttgttttc acgcacgtag ccgcggtaga
    21721 catcgcatga gataaccgaa agctcatcca taatgcgacg ccagctctct ttcggctctg
    21781 gcggtggcag caggttggct tccagaatcg ccccggtata cagcgaaagg ctgctaacgg
    21841 tgatttcagg cagaccgtac ttgaagcgga tcatctcgcc ctgttcggtc acgcgcaggc
    21901 cgcctttcag gcttcctggt ggttgtgaca gcagcgccgc atgggcaggt gcgccgccgc
    21961 gaccaatgga accgccgcga ccgtggaaca acgttagctc aatacccgct ttttcgcagg
    22021 ttttgattaa tgcatcctgt gcctgatatt gcgcccagga agctgccatc acgcccgcat
    22081 cttttgctga gtcggaatag ccaatcatca ccatctgttt gccctgaatc aggccgcgat
    22141 accagtcgat attcagcagc tgggtcatga catcgttggc gttgttcagg tcatcgaggg
    22201 tttcaaacag cggagcaacc ggcatcgcaa acccgatacc cgcttctttc agcagcaggt
    22261 ggacagccag cacgtcggac ggcgttttcg ccatcgagat cacgtaggcg gcaatggagc
    22321 cttgcggtgc ttcggcaatc acctggcagg tatcgagcac ttcgcgcgtt tcggcgcttg
    22381 gttgccagtt gcgcggtaga agcggacgtt tggagttcag ttcgcggatc aggaacgcct
    22441 gtttgtcggc ttctgaccag ctctcgtagt cgccgatacc gaggtagcgg gtcagctcgc
    22501 ccagcgcttc ggtatggcgg gtgctctcct gacggatatc aatacggacc agcggtacgc
    22561 cgaaacattt cacgcggcgc agggtgtcga gcagatcgcc gttggcgata atacccatgc
    22621 cacacgcctg aagtgactgg tagcaagcgt agagcggttc ccacagttct tcgttttgtg
    22681 tcagcaggcc ttctggtttt ggcagttctt cgcctttcag gcgcgcttcc agccatgcct
    22741 gtgtcgccat caggcgagaa cgcaggtttt tcatcagata gcgatacggt tctgcggcac
    22801 cttcttcgcc aaccagcgcc agcagttcag gggtcgcttc aaccatcgac agttcagaaa
    22861 ccagcacctg aatatctttc aggaacagat cggtggcttt ccagcgactg agcagcagga
    22921 cgtggcgggt gatatcggca gtgacgttcg ggttgccgtc gcggtcaccg cccatccacg
    22981 aggtaaaacg gaccggaaca aattcgacgg gcagtttgta gccgagattc tcttccagtt
    23041 gttcgttcag ttcgcgcagg taattcggta cgccttgcca caggctgttt tctactacgg
    23101 caaagcccca tttggcttca tctaccgggc ttggacgcag cttacggatt tcatctgtat
    23161 gccatgactg ggcgatcaac tggcgcaggc ggcgcatcag ctggttatgt tcgtagtcgg
    23221 cgatatcttt gttatcgagc tgttttaagc aggcgttcac ttccaccatt ttgtggatca
    23281 gtgtacgacg ggtaatttcg gttgggtgag ccgtgaggac cagttccagc gacagcgatt
    23341 ccactgcttt tttgatggtg tcttcgctca gttccggctg gtttttcagt ttacgcaggg
    23401 tgcgggcgat cacttccggg ttgctggcag cttcgccttt cggcgaaatg ctgtggtatt
    23461 gctcagcggt gttggccagg ttcaggaact gactaaacgc acgcgcaacg ggcagcagct
    23521 cgtcgttcga caaattttgt aaggtggtga gcaactcctg gcggttagca tcattgccag
    23581 cgcgtgaaga tttcgacaac ttacggatag tttctacgcg ttcaagaatg tgttctccca
    23641 acgcatcctt gatggtttct cccagcactt tgccgagcat actgacatta ctacgcaatg
    23701 cggaatattg ttcgttcata ttaccccaga caccccatct tatcgtttga tagccctgta
    23761 tccttcacgt cgcattggcg cgaatatgct cgggctttgc ttttcgtcgt cttttataaa
    23821 gccacgtaaa agcggtgacg tcaaatgctg cgaaatcgct tcagcaaacg aataaatagc
    23881 aggaatttac gccattaaat tcatgacgct ttaaataagc gtaacttatg ggaatgttaa
    23941 aaaatcgcct cgagtaacac caaaggtgta ggtcggataa gatgcgtaag catcgcatcc
    24001 gacattgttt ggcctacgtt ttaatgccag caaaaatggt gaatcacctg ggtgatcagt
    24061 tcgcgggtgg gcttgataaa cctcgtctcc agatattcat caggctgatg agcctgatta
    24121 attgagccag gccccagaac cagcgtcggg cataacgttt gaataaacgg cgcttcggta
    24181 cagtaattca ccacttcggt ctttgctccg agcaatttct caaccacttc aaccagttta
    24241 tgattcggtg ggcattcata gccagggatc ggcggatgca gctcgtcgac cgtcagacga
    24301 cccggccagc gttcgctcac tggagccaat gcatcgttga gcaaaccatt aagttcattg
    24361 agtgtcatgc ctggcagcgg acgaatatcc atatgcaact cacagcaagc acaaatacgg
    24421 ttagaagcgt cgccaccgtg gatatgcccg aggttgagcg tagggtatgg cacggtaaac
    24481 gcgtcgtagt gataacgttc tttcaggtta tcgcgcaatt gcaaaatatg cccgatggcg
    24541 tcgtgcatca gttcgatagc gttaactccg cgtgctggat cgctggagtg gcccgactgg
    24601 ccctgaatac ggatggcgtt agagatatga cctttatgcg cgcgtaccgg ttgcagcgac
    24661 gttggctcgc caatgatggc gcaatccggg cgcagggcgg tagtttcggc aaaataacgc
    24721 gctccggcca tactggtttc ttcatcggca gtcgccagaa tgtagagcgg ttttgcaagc
    24781 ttcgtgacgt cgacatcgcg tagcgcatca aggataaacg caaaaaagcc tttcatgtcg
    24841 gcggtgccta agccgtaaag cttgccgtca tgctccgtca acgtaaatgg atcgcgcgtc
    24901 cagcgtccgt catcaaatgg caccgtatcg gtatgccccg ccagcaacaa accgccagcc
    24961 ccctgtccgc aactggccag catattaaat ttgttgcgag ttcctggcac aggctgtact
    25021 tccacattga agcctaaatc cttaaaccag tccgccagca gagtgattaa atctgcattg
    25081 ctttgatcga gtgcctcttc cgtggcgctt attgaaggtg tggcaatcag agcacggtaa
    25141 atctcgataa atggcggtaa tttgtttttc attgttgaca cacctctggt catgatagta
    25201 tcaatattca tgcagtattt atgaataaaa atacactaac gttgagcgta ataaaaccca
    25261 ccagccgtaa ggtgaatgtt ttacgtttaa cctggcaacc agacataaga aggtgaatag
    25321 ccccgatgtt gaatacgctg attgtgggtg ccagcggcta cgctggcgca gagctagtga
    25381 cctatgtaaa tcgccatccg catatgaaca taaccgcttt gactgtttca gcgcaaagca
    25441 atgatgcggg aaagttaatc tccgatttgc atccgcagct aaaaggcatc gttgatctgc
    25501 cgttgcagcc gatgtcggat atcagcgagt ttagcccagg ggtggacgtg gtgtttctcg
    25561 ccaccgccca tgaagtcagc cacgatttag cgccgcaatt tcttgaagca ggctgtgtgg
    25621 tgttcgacct ttccggcgcg tttcgtgtta acgatgtcgc cttctatgaa aaatattacg
    25681 gctttaccca tcaatacccg gaactgctgg aacaggcagc ctacggtctg gcggagtggt
    25741 gcggtaataa attaaaagaa gcgaacttga ttgccgtgcc aggctgttat ccgacggcag
    25801 cacagctggc gctgaaaccg ttgattgatg ccgatcttct tgacctcaat cagtggccgg
    25861 tgatcaacgc caccagcggc gtgagcggtg caggacgtaa ggcagcgatt tcaaatagtt
    25921 tttgtgaagt tagcctgcaa ccgtatggcg tctttaccca tcgccatcaa ccagagatcg
    25981 ccacacacct cggtgctgac gttatcttca ccccgcatct gggcaatttc ccgcgcggca
    26041 ttctcgaaac cattacctgc cgcctgaaac cgggtgtgag ccaggtgcaa gtggcgcaag
    26101 cgttacagca ggcgtatgcc cataaaccac tggtgcggct gtatgacaaa ggcgttccgg
    26161 cgctgaaaaa tgtcgttgga ctgccattct gcgatatcgg ttttgccgtt cagggcgagc
    26221 atctgattat tgtggcgacc gaagacaact tactgaaagg cgcggcggca caagcggtgc
    26281 agtgcgccaa tattcgtttc ggctatgcgg aaacgcagtc tcttatttaa gggtgcaatg
    26341 atgaatccat taattatcaa actgggcggc gtactgctgg atagtgagga ggcgctggaa
    26401 cgtctgttta gtgcactggt gaattatcgt gagtcacatc agcgtccgct ggtgattgtc
    26461 cacggtggcg gctgcgtagt ggatgagttg atgaaagggc tgaatctgcc ggtgaaaaag
    26521 aaaaatggcc tgcgggtgac gcctgctgat cagattgaca ttatcaccgg agcactggcg
    26581 ggaacggcaa ataaaaccct gctggcatgg gcgaagaaac atcagattgc ggccgtaggt
    26641 ttgtttctcg gtgacggcga cagcgtcaaa gtgacccagc ttgatgaaga gttaggccat
    26701 gttggactgg cgcagccagg ttcgcctaag cttatcaact cattgctgga gaacggttat
    26761 ctgccggtgg tcagctccat tggcgtaaca gacgaagggc aactgatgaa cgtcaatgcc
    26821 gatcaggcgg caacggcgct ggcggcaacg ctgggcgcgg atctgatttt gctctccgac
    26881 gtcagtggca ttctcgacgg caaagggcaa cgtattgccg aaatgaccgc cgcgaaagca
    26941 gaacaactga ttgagcaggg tattattact gacggcatga tagtgaaagt gaacgcggcg
    27001 ctggatgctg cgcgcacgtt gggccgtccg gtagatatcg cctcctggcg tcatgcggaa
    27061 cagcttccgg cactgtttaa cggtatgccg atgggtacgc ggattttagc ttaagttttg
    27121 ttggccggag gcgcagcttt ccggcattga atttcaaaat aaggaaacag agttatggca
    27181 ctttggggcg ggcgttttac ccaggcagca gatcaacggt tcaaacaatt caacgactca
    27241 ctgcgctttg attaccgtct ggcggagcag gatattgttg gctctgtggc ctggtccaaa
    27301 gccctggtaa cggtcggcgt gttaaccgca gaagagcagg cgcaactgga agaggcgctg
    27361 aacgtgctgc tggaagatgt tcgcgccagg ccacaacaaa tccttgaaag cgacgccgaa
    27421 gatatccata gctgggtgga aagcaaactg atcgacaaag tgggtcagtt aggcaaaaaa
    27481 ctgcataccg ggcgtagccg taatgatcag gtagcaactg acctgaaact gtggtgcaaa
    27541 gataccgtta gcgagttgct gacggctaac cggcagttac aatccgctct ggtcgagacc
    27601 gcgcagaaca atcaggacgc ggtgatgccg ggttacactc acctgcaacg cgcccagccg
    27661 gtgacgttcg ctcactggtg cctggcctac gttgagatgc tggcgcgtga tgaaagccgt
    27721 ttgcaggacg cgcttaagcg tctggatgtc agcccgctag gctgtggcgc actggcagga
    27781 acggcctatg aaatcgaccg tgaacagtta gcaggctggc tgggctttgc ttcggcgacc
    27841 cgtaacagtc tcgacagcgt ttctgaccgt gaccatgtgc tggaactgct ttctgctgcc
    27901 gctatcggca tggtgcatct gtcgcgtttt gctgaagatc tgattttctt taacaccggc
    27961 gaagcggggt ttgtggagct ttctgaccgt gtgacttccg gttcatcatt aatgccgcag
    28021 aagaaaaacc cggatgcgct ggagctgatt cgcggtaaat gcggtcgggt gcagggggcg
    28081 ttaaccggca tgatgatgac gctgaaaggt ttgccgctgg cttacaacaa agatatgcag
    28141 gaagacaaag aaggtctgtt cgacgcgctc gatacctggc tggactgcct gcatatggcg
    28201 gcgctggtgc tggacggcat tcaggtgaaa cgtccgcgtt gccaggaagc ggcgcagcag
    28261 ggttacgcca acgctactga actggcggat tatctggtgg cgaaaggcgt accgttccgc
    28321 gaggcgcacc atattgtggg tgaagctgtg gtggaagcca ttcgtcaggg caaagcgttg
    28381 gaagatctgc cgctcagtga gttgcagaaa ttcagccagg tgattggcga agatgtctat
    28441 ccgattctgt cgctgcaatc gtgcctcgac aagcgtgctg caaaaggcgg cgtctcaccg
    28501 cagcaggtgg cgcaggcgat tgcttttgcg caagctcggt tagagtagtt tgtttatatt
    28561 gctcccctga aaatggggag cttaccacgc ctacaaagtg cgacacttcg taggcggata
    28621 aggcgtttac gccgcatcag gcagaaattt gtcagtattc tggtgacctt aaatttaacg
    28681 cccaaccaga accattattt gtcacattcc tttttattct tgcgtcttct ttaccttcac
    28741 tctccccata aaaattatat
//
LOCUS       DAZGHF010000056        28125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGHF010000056 DAZGHF010000000
VERSION     DAZGHF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.056"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            71..802
                     /gene="artJ"
                     /locus_tag="ACHK3A_002491"
     CDS             71..802
                     /gene="artJ"
                     /locus_tag="ACHK3A_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO8740554.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(831..1958)
                     /gene="rlmC"
                     /locus_tag="ACHK3A_002492"
     CDS             complement(831..1958)
                     /gene="rlmC"
                     /locus_tag="ACHK3A_002492"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO8740555.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLSVKHMWDLFCGVGGFGLHCAMPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYCIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(1999..2487)
                     /locus_tag="ACHK3A_002493"
     CDS             complement(1999..2487)
                     /locus_tag="ACHK3A_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO8740556.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2547..3392)
                     /gene="potI"
                     /locus_tag="ACHK3A_002494"
     CDS             complement(2547..3392)
                     /gene="potI"
                     /locus_tag="ACHK3A_002494"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO8740557.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDNAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(3389..4342)
                     /gene="potH"
                     /locus_tag="ACHK3A_002495"
     CDS             complement(3389..4342)
                     /gene="potH"
                     /locus_tag="ACHK3A_002495"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO8740558.1"
                     /translation="MSTLEPAAQPKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4352..5485)
                     /gene="potG"
                     /locus_tag="ACHK3A_002496"
     CDS             complement(4352..5485)
                     /gene="potG"
                     /locus_tag="ACHK3A_002496"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO8740559.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASWVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPADGFNFAVGEVMHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRYRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5580..6692)
                     /gene="potF"
                     /locus_tag="ACHK3A_002497"
     CDS             complement(5580..6692)
                     /gene="potF"
                     /locus_tag="ACHK3A_002497"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO8740560.1"
                     /translation="MTALNKKWLSGLVTGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7043..7519)
                     /locus_tag="ACHK3A_002498"
     CDS             complement(7043..7519)
                     /locus_tag="ACHK3A_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO8740561.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7607..8509)
                     /gene="rimK"
                     /locus_tag="ACHK3A_002499"
     CDS             complement(7607..8509)
                     /gene="rimK"
                     /locus_tag="ACHK3A_002499"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO8740562.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8570..9292)
                     /gene="nfsA"
                     /locus_tag="ACHK3A_002500"
     CDS             complement(8570..9292)
                     /gene="nfsA"
                     /locus_tag="ACHK3A_002500"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO8740563.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENNYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9276..9563)
                     /locus_tag="ACHK3A_002501"
     CDS             complement(9276..9563)
                     /locus_tag="ACHK3A_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO8740564.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9723..9980
                     /gene="grxA"
                     /locus_tag="ACHK3A_002502"
     CDS             9723..9980
                     /gene="grxA"
                     /locus_tag="ACHK3A_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO8740565.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10010..10387)
                     /locus_tag="ACHK3A_002503"
     CDS             complement(10010..10387)
                     /locus_tag="ACHK3A_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO8740566.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10657..12342
                     /locus_tag="ACHK3A_002504"
     CDS             10657..12342
                     /locus_tag="ACHK3A_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO8740567.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12518..13054)
                     /gene="rcdA"
                     /locus_tag="ACHK3A_002505"
     CDS             complement(12518..13054)
                     /gene="rcdA"
                     /locus_tag="ACHK3A_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO8740568.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13138..14346
                     /locus_tag="ACHK3A_002506"
     CDS             13138..14346
                     /locus_tag="ACHK3A_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8740569.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14346..15161
                     /gene="ybjI"
                     /locus_tag="ACHK3A_002507"
     CDS             14346..15161
                     /gene="ybjI"
                     /locus_tag="ACHK3A_002507"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO8740570.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFSTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            15246..15530
                     /gene="ybjH"
                     /locus_tag="ACHK3A_002508"
     CDS             15246..15530
                     /gene="ybjH"
                     /locus_tag="ACHK3A_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO8740571.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15571..16803)
                     /gene="mdfA"
                     /locus_tag="ACHK3A_002509"
     CDS             complement(15571..16803)
                     /gene="mdfA"
                     /locus_tag="ACHK3A_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO8740572.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHVWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            17088..17684
                     /gene="ybjG"
                     /locus_tag="ACHK3A_002510"
     CDS             17088..17684
                     /gene="ybjG"
                     /locus_tag="ACHK3A_002510"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO8740573.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWSMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17742..18500
                     /gene="deoR"
                     /locus_tag="ACHK3A_002511"
     CDS             17742..18500
                     /gene="deoR"
                     /locus_tag="ACHK3A_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO8740574.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18547..19749)
                     /gene="dacC"
                     /locus_tag="ACHK3A_002512"
     CDS             complement(18547..19749)
                     /gene="dacC"
                     /locus_tag="ACHK3A_002512"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO8740575.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            19996..20622
                     /gene="gstB"
                     /locus_tag="ACHK3A_002513"
     CDS             19996..20622
                     /gene="gstB"
                     /locus_tag="ACHK3A_002513"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO8740576.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20619..21734)
                     /gene="yliI"
                     /locus_tag="ACHK3A_002514"
     CDS             complement(20619..21734)
                     /gene="yliI"
                     /locus_tag="ACHK3A_002514"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO8740577.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESDARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21845..22228)
                     /gene="bssR"
                     /locus_tag="ACHK3A_002515"
     CDS             complement(21845..22228)
                     /gene="bssR"
                     /locus_tag="ACHK3A_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO8740578.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22441..23766
                     /gene="rimO"
                     /locus_tag="ACHK3A_002516"
     CDS             22441..23766
                     /gene="rimO"
                     /locus_tag="ACHK3A_002516"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO8740579.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            23992..24378
                     /locus_tag="ACHK3A_002517"
     CDS             23992..24378
                     /locus_tag="ACHK3A_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8740580.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVIQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            24368..24697
                     /locus_tag="ACHK3A_002518"
     CDS             24368..24697
                     /locus_tag="ACHK3A_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8740581.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(24767..26095)
                     /locus_tag="ACHK3A_002519"
     CDS             complement(24767..26095)
                     /locus_tag="ACHK3A_002519"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO8740582.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQLPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(26103..28125)
                     /locus_tag="ACHK3A_002520"
                     /pseudo
     CDS             complement(26103..28125)
                     /locus_tag="ACHK3A_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT         7265 a         7215 c         6704 g         6941 t
ORIGIN      
        1 ttgatggcat tgttatccca tgccgcagac acggccaaaa atcataagat tgacagacgg
       61 gagttccatc atgaaaaagt tagttcttgc cgcattactt gcttccttta ctttcggtgc
      121 ttctgccgca gagaaaatca attttggcgt ttcagccacc tatccaccct ttgaatctat
      181 aggtgctaat aatgagattg tcggctttga tatcgacctg gcaaaagcct tgtgcaaaca
      241 aatgcaggca gaatgtactt ttactaatca cgcgttcgac agcctgatcc cgtcactgaa
      301 attcagaaaa tatgacgccg taatctccgg tatggatatc actccagagc gtagcaaaca
      361 ggtatcgttt accacgccct actatgaaaa ctcagccgtc gtgattgcca aaaaagatac
      421 ctacaaaacg tttgccgatc tgaaaggcaa acgtattggg atggaaaacg gtactacgca
      481 ccagaaatat attcaggatc agcacccgga agtgaaaact gtctcctatg acagttatca
      541 gaatgccttt atcgatctga aaaatggccg tattgatggg gtatttggtg acacagcggt
      601 ggttaatgaa tggctgaaaa ccaacccgca actgggcgtt gcgactgaaa aagtgaccga
      661 tccgcaatac ttcggcaccg gcctgggtat cgctgtacgt ccggataaca aagctctgct
      721 ggaaaaactg aataacgcgc tggcagcaat taaagctgac ggtacatatc agaaaatcaa
      781 tgaccagtgg ttcccgcagt aagattcaga aagccgcacc tgcggctttt ttattgctta
      841 accagcagtg tcagcacttc gtaatgcgca gtgtgcggga acatatcaaa aagctgtacc
      901 cgttcgatgc agtaacctgg cagttcgcgg atatctttcg ccatggtttg ggcgttacag
      961 ctggagtaga taataaaacg cggtgccatc gttgagagat aatcacacag cggtttacca
     1021 atgccgcggc gcggcgggtt aaccagcacc agctccggca cctccccctg agcagtggca
     1081 aactgagtgg agtccagcgc ctgaaattgc aaacgcgtta agcccagttc agcggctgac
     1141 tgctttgcac aggcaatggc ctctggcgca atttcgatcc cggttaactg catgtcaggc
     1201 atcgcgcagt gtaaaccaaa gccccccaca ccgcagaaca gatcccacat atgtttaacc
     1261 gacagctgtc gtacccagtc gcgcgcggtg gcgtacaact ggctggcgac cgccggatta
     1321 gtctggaaga aactttgcgg acggatccac agcggtacgt cattaaaacg ctctgccagc
     1381 gcctgttgtt cagtcaggta aatctccgtc tccccttcca taatcgccat atgtaccggc
     1441 tgaatattga cggtaataac tttcagctgc ggtagttgtt cctgtaacca cggcagcgcc
     1501 ttacgcagtt gcgccagctt ggtatcagaa cgcagtacaa agcgcagcat catgccgcca
     1561 tcgctctggc tttcagtcag cagaatgtat ttcagttcgc cacgcttacg cgccacgttg
     1621 tagggcgtta accccgctcg ggcgataaac ggttttagcg ccgcaaaaac gggcgcaaat
     1681 gaggcaggat aaagcggaca gtcacaaagg tcttccggcg tgccgtcgcg atgcagcata
     1741 ccgagcagtg gtttttcaac gctaccactc accaccattt tggctttatt acgaaaccct
     1801 tgttccgggc ctgacaccgg cgcgcaccat tcctcaaccg gaaagtcggc gagcagattt
     1861 ttaagatcgg cggttttagc ggagagttgc tctggaatcg gctgcgttat ccactgacag
     1921 gaacgacagc gacccgcgtc gtaaagtgcg cactgcatac attgaccttc acatcatcag
     1981 gggcgacgat tatacacatt attgcaactg gaagaaccgc cgactggtcg agggaacgaa
     2041 cagcagcatc aggatgagca tatccggcag cttctgcagc atcaggctat ggaagatttc
     2101 acgttttgat tcaccgggaa tgctgaacag ctccggataa ccatacccca gcgaagctgc
     2161 ccacaagtaa cttgcggccg tgatttgtgt caacagatac agccagcgcg cccagcgacg
     2221 ccctttcacc agtgaaaacg cacaccagat ctcaatgaaa accagcacca gactgctcaa
     2281 aaagaccagc gttaaactcc aggtttgtac gctgcgatga atgaactcgc caataccgcg
     2341 cacgcccagc gtattgaaaa tcatcagcac gtcgaggcca cggatcataa taatggcgag
     2401 cgccgccacc tgcaccagcg caggcacatt caggcgagca tgagatgaag atgttttctt
     2461 aaaaaatccc aacgtttcgt cttccatgaa aacaatgccg cgacatgcgc ggcattatgt
     2521 agccaggttg gcaaatttta gtgtcttcag ccacgtcttg cacgctggat gtcgcgtatc
     2581 cgctgttttt ctgcgcgcgc catcagatac caggcgataa atccgacaat tccgaccgcg
     2641 ccgaggatca atgttgccag ggcgttgatt tccggattca cccccatccg cacgctggaa
     2701 aagaccagca tcggtaaagt ggtggctccc ggcccggaaa caaagctggc gatcaccaga
     2761 tcatcaagcg acaaagtaaa agccagtaac cagccagaaa tgatcgcggg catgatcatc
     2821 ggtagcgtaa tgacaaaaaa cactttcagc ggcgtcgcac cgagatccat cgcggcttct
     2881 tctatcgagc gatccagttc ccgcagacgc gacgaaataa cgactgccac ataagccgta
     2941 cagaaagtga catgcgccag ccagatggtg agcataccgc ggtccgcagg ccagccaata
     3001 gcatgagcaa gcgcgacgaa taacaacaat agcgacaagc ccgtgatgac atctggcatc
     3061 accagcggcg cggtgatcat aaaggcaaaa ccatttgatc cgcgaaacct accaaagcgc
     3121 accagcacga ccgccgcaat cgtcccgagg atcgccgccg ccgtcgccgc acaggccgca
     3181 attgtcaggc ttaaaccaac cgcactcatc atcgcgttat cgcgcaataa ctcaccatac
     3241 cagcgtgttg accagccggc ccacaccgtc accagtttcg agctgttgaa tgaatagatc
     3301 accagcatca gcattggcgc gtagagaaag gtgaagccca gcagcaaaat cacaatccgc
     3361 cagggcgaac gaacaaccgg taaattattc atccgtgttc tcccacgctt ttttgctggt
     3421 gtttgtgaaa ccacattatc ggcacaatta gcagcagcaa catgatgatc gctaccgccg
     3481 aggccaccgg ccagtcgcgg ttattgaaaa actcctgcca cagcacgcgc ccgatcatga
     3541 tgctgtccgg gccaccgagc agttcaggga tcacaaactc gcccacagcc gggataaaca
     3601 ccagcatcga tccggcaata atcccgcctt tggtcagcgg cacgatcaca gtaaagaacg
     3661 ttttcagcgg tcgtgcaccg agatccagtg ccgcttccac cagcgaataa tcaatacgaa
     3721 tcaaggcggt gtagatcggc agcaccataa acggcacata ggcgtaaaca atgccgatat
     3781 aaaccgccag gttggtatgc agaatggtca acggttgatc gataaccccc agccacagca
     3841 gaaaattatt cagcacaccg ttgtttttta atattcccat ccaggcataa acgcggatca
     3901 gaaacgaggt ccacgacggc aggatcacca gtagtaataa gatattacgg gtcgaaggct
     3961 tactgtgcgc caccgcccac gccagcggat agccgatcag taaacagcaa aatgtcgaaa
     4021 tcgcagccac ctgtagcgac tggagataag catcgaaata gagcggatcg tcagtcagtt
     4081 gcagaaaatt accgagatta agagtgatgg acagttgccc gtcagcccac tccatcagtt
     4141 cggtataagg cggaatggcg cgcgccatct ccgccagact tattttaaag acgatcagaa
     4201 atggcagcag aaacagcagg atcaaccaga tatacggcaa cgcaatgacc agtttgcgcc
     4261 catgcttcat ttgcagctgc gacaaccaca gcttaaatcc gcccggcggt ttgggctggg
     4321 cagcaggttc aagtgtactc atctttgctc cttaaaccgt cagcaccaca cagctgtcca
     4381 cttcccagca caaacgcact tcgtcgcccc aggttggtaa ccctttgcga tatcgatgag
     4441 cgttttgtag ctgagcgctg atcatctgtc cgctcttgag acgaacgtga tacaccgaaa
     4501 gatcgccgag ataggcaatg tgcatcacct cacctaccgc aaagttaaaa ccgtcagcgg
     4561 gaggttcctc gcaaagcatg attttttccg ggcgcagcgc cacatgcacc ggcacattat
     4621 cgaccaccga agcatctaca tcgactttca gtggatgcac cagccccggc gaataaagca
     4681 ccagaccatc ttcctgacgc tctttgagta caccttcaaa gacattaacc gagccaataa
     4741 attcggcgct gtagcgagtg gtcggatgct cgtagatctc ttccggttcg ccaatctgga
     4801 caaatttccc tcgattcata atggcgatac gccccgccat ggtcatcgcc tcttcctgat
     4861 cgtgggtgac catcacacaa gtcacaccga cacgctccag aatatccacc acttcaagct
     4921 gcatccggtc acgcagcttt ttatccagcg cgcccatagg ctcatcgagc agtaatagtt
     4981 tcgggcgctt ggcaaggctt cgggccagtg ccacacgctg tcgctgaccg ccggaaagct
     5041 gatgcggttt gcgtttggcg aactcctgca tatgcaccag cccgagcatc tcattgaccc
     5101 agctggcaat ttccgctttc ggtagtttgt cctgtttcag gccaaaagcg atgttctgtt
     5161 ccacggtcat atgcggaaac agcgcgtaag actgaaacat catatttatg gggcgcaggt
     5221 aaggcggaac ctgtgacaaa tcgacgccat caagcattat ctgtccggca gaaggttgtt
     5281 cgaaacctgc cagcatacgc agcagcgtgg acttgccaca gccggatgcg cccagcagcg
     5341 cgaagatttc gcctttatag atggtcaggc tgacatcatc gaccgcatgc tgaccatcgt
     5401 aagatttagt cagattacgg atttctaata gcggcgtcag caccttacgg gttttcgcct
     5461 gcgggcgagg gatagcgtca ttcacggctg ctctccggca ttgaatgtgt gcaagcgcac
     5521 cataatggtg cgaattgccg gcgggcgtgg tgcgcccgtc cggcatctac gactgcggat
     5581 tattttccgc tcttcacttt ggtccacgcg cgggtgcgca cacggtcgat tttcggatcc
     5641 tgtactttca gagtgaacag cttcgcacga acatccgcag gcgggtaaat acccgggtta
     5701 tcacggactt ccgcactcac cagcggcgta gctgctttat tggcgttagc atagaacaca
     5761 tggtcggaaa tatgcgccac cacatccggg cgcagcaggt aattcaggaa ctgataggct
     5821 tcgtctttat ttttggcatc cgcaggcatg gcgaagacat caaagaacgc catcgcccct
     5881 tctttcggaa tcgagaacga gacattaacg ccattcttcg cttccttcgc gcggtttgac
     5941 gcctgccaga catcgcccgc ccagccaata gcgacgcaga tatcgccgtt tgccaggtcg
     6001 ttaatgtatt gagatgaatg gaaataacga atgttcgggc gcagctttaa cagcagatcg
     6061 gttgccggtc cggtgtaatc atctgcttta gtgctgttgg gatctttacc gagataattc
     6121 agcacggtag caaacacttc ttccggtgca tcaaggaaag agacgccgca gcttttcagt
     6181 ttttccagat tttcaggctt gaggatcaag tcccagctat cgacgggcgc gttttcgccc
     6241 agcaccgctt taactttatc aacgttatag ccaatcccgg tcgtcgccca catatagggc
     6301 atagcaaatt tattgtcggg atcgtgtttg gcaactaact tcagcagctc cggatcgaga
     6361 ttcttccact ccggcaattt gcttttgtcc agcggctgga aaactcccgc agtcaactgg
     6421 cgttcaagaa agcttgctga cggcaccacc aggtcaaagc cggtgctacc cgccattaat
     6481 ttgccttcca gtacttcgtt agagtcgaaa acatcgtaga cgactttaat accggtttct
     6541 ttttcaaaat tggccaccgt gtccggggcg atataatcag accagttata aatgtgaagt
     6601 gttttttgtt cagccgcgag cgtgccgaca gagacggcca tcagagcacc cgtaaccaga
     6661 cccgatagcc attttttatt taaggcggtc atatctcttt ccttctgaaa gttcgttaac
     6721 aaacgaacaa aagttgtcac actgaaagat atgcaataaa cgtgcatatt ttttatcggc
     6781 gcggtgtaag aaaagagaac tctctcccgg ccagcactgc tcgctatata aaagcatcgc
     6841 aagaataact ttagccaggg tttgagacta tagctcggat taaaaaacgg tatttaacaa
     6901 atttttatgc gtatttgctc tatgtgataa agccaaatcg gcacaagaga agtgaaataa
     6961 tctttaagaa aaggttaaga atagcagata attttgcagc acgcagtcac tgcggcagcg
     7021 actgcgtact cgaaaggagt tatcagtgga ggaaatagtt ttcggtaacg ttgttttgcc
     7081 cttcatcgtc agtcggtaac agcagttgat gggcattagc ttcaagaatg accatcgaaa
     7141 tctgctcttc gctctgacgc acgaaccatg caaactgctc ataggttacg ccctgcataa
     7201 cggataaaga ctggcaaacc accagctttg gcagattatc atcctgcata tcaagaaatg
     7261 ctttcacggt cagcgaactg gcattgatgg cagataaatc cgccgccagc ggcaatacgg
     7321 ctgaaggtcg gacttccgcc atggcagaaa acaggatcgt gttatcgatc agatcgattt
     7381 tggcatcaaa gacaccgtcg aaattctgca tatggggcag atgcagagcc tgacagttat
     7441 cacattcaaa aaaactcatc cccaggtcat cgagccattg acgcagcgta tccagaccag
     7501 gaacgaccag cgatgtcata attttgtaca acctctttcg ataaaaagac cggcacagct
     7561 tacgcaaaaa gcgcaggcaa aaccatgatc agtaatgtga ttgcgactaa ccacccgttt
     7621 tcaggcaata ttccgtcgta gcgtggcgtt cgatccagcg gatcatttta cccgcgatgt
     7681 cgataccggt ggttttttct attccttcca gccccggcga cgcattcacc tccatcacca
     7741 atggcccgcg attagcacgc agaatatcca caccagcaac gtccagcgcc atcgttcgcg
     7801 cggcttttat cgcgatttca cgctcctgtg gtgtgatact ggcgacgctt gccgcgccgc
     7861 cacgatgcaa attggaacga aaatcgccct ctttcgcccg ccgttcaatc gcagcgacca
     7921 cttcatcgcc aacaaccaga cagcggatat cgcacccttg cgcctctttg atatattcct
     7981 gcaccagaat atgcgcgttc agaccgcgga aagcgtcaat cacgctttcc gccgcctggc
     8041 gcgtctccgc cagcacgaca ccaattccct gcgtgccttc aaccagcttg accaccagcg
     8101 gcgcaccacc gaccatgtcg attaaatcgc tggtatcatc cggcgaatgc gcaatgcccg
     8161 tgacaggcag gtcgatgccc tgacgcgcca gcagttgcat ggaacgcaat ttgtcacgcg
     8221 cccgggcaat ggcgaccgac tcattgagcg gatagctccc cagcatctcg aactggcgca
     8281 gtgccgccgt cccataaaag gtaatggcgg taccaatacg cgggatcaca gcatcaaaat
     8341 ggggtaactt gcggcctttg tagtgaatag aagacgccgc aggatttatg ttcatgtagc
     8401 aagaaagcgg atcaagaatt tcaaccaggt gaccgcgctg tatagcggct tcacgcagcc
     8461 gcttacacga atagagcgtt ccatcccggg acaatatggc aattttcacc ctgcacctct
     8521 ctgtcagacc tggtcaaaag cccgcgtatc atacatcggc gtggcggttt tagcgcgtcg
     8581 cccaaccctg tttgtgcaaa taatccagta taaatgggcg gctttcttta atgattgttc
     8641 ggcggatatg atcgctccag gtatcccggc gattattgct gccacgggtg aggtaatatt
     8701 ccgccagttg ttcgtcatac tgcgccagtg cgtctttatc cagcggttga tagttgtttt
     8761 catgcaccaa aatggaggcc ggtaaacgcg gcttaagatc cggattatcc gcaggccagc
     8821 caaggcacag cccaaacagc ggcagaacat gctgcggtaa tttcagcagc tccgtcaccg
     8881 cttcaatatt attgcgcagg ccgccgatat ataccccgcc caatcccagc gattccgctg
     8941 cggttaatgc attctgcgcc atcattgccg tatcaacgac accgagcaac agttgttccg
     9001 ccaggccaag ttgtgcatcc ggacagatct gtaaatggcg gttaaagtcg gcacagaaca
     9061 cccagaactc cgccgcttgc gctacgtgtt tttgcccgcc ggtcagcgtc accagttcat
     9121 cacgtaacgc tttgtcggta atgcgaataa tgctactgca ctgcaaaaaa ctggaactgg
     9181 acgtcgcacg ggcgctgtta ataatcgcct cacgctgcgc ttcggaaatg ggttcatcag
     9241 tgaaatggcg aatggagcga tggccacaaa taagttcaat ggttggcgtc attatctttt
     9301 tctctttctg aacgtgaata ttgcggtgga cggttcatca gctgtggggc aagacgtttt
     9361 gccacctgaa gaataaccac caccgcagcg ggaagcatga gcaaaacacc gagaaaaatc
     9421 atcagaatct gcacttctgg ccgagaaaat ggctcaggca gcgacaggga gccgcttacc
     9481 gacagcagcg ccaccgccag caacatcatt ccgataaatt ccagtatcaa cacgctttta
     9541 ggcaatttac cgatcgcgcg catacgcttc cctctgcaaa gtgagccttc agtctaaaac
     9601 ttttcactgt attgtgttta acagttatag cttttagcaa ttaatgcaac aggttaaacc
     9661 tactttcagc gaatacattt tagcgtgatc attacaggta taaatctatg aggagagaaa
     9721 taatgcaaac cgttattttt ggtcgtccgg gttgccctta ctgtgtgcgt gcaaaagatc
     9781 tggctgagaa attgagcaat gaacgcgatg atttccagta tcagtatgta gatattcgtg
     9841 cggaagggat cactaaagaa gatctacaac aaaaggcagg aaaacccgta gaaaccgtgc
     9901 cgcagatttt tgtcgatcag caacatatcg gcggctatac cgattttgct gcatgggtga
     9961 aagagaatct ggacgcctga tcgtctgaca agccctcgcg ttgagggctt tattgatttt
    10021 ttctgtgctg tggtttaaac aaactactga taaataagaa acacaatgcc cccagcgcac
    10081 accagaacac cgcacttaat aaccatgcca gctcttgcca gaatgagcgc gtcggtgaaa
    10141 aaaacagccg cataatgagc atcgaacagg gtgccgccag cattgcgcca aacagaggtt
    10201 tcaggacttc tctacgatgt gaaaagaagc tggcaactgc accaggaaga atgaaaaata
    10261 gcaaaccgat ttcaggatgc ccggcagccc gaaaagcgcc tttcatgtgc gtcgccagaa
    10321 aaagacacac cacaatgaag aggacaaaac agcagattgc ccccgcccaa cgttgtttat
    10381 gtttcactcg ttcctcctga cactgcgtct atcgaacaca tttttcgcca gtgtggcgtt
    10441 cagtaagata aagccgcttc gcattccatg ctaatatagg ccaacgcaat tcatatagcc
    10501 gttgatacct aatgtgatta cactagtaaa atatattgtt actttactat cgtttaggtg
    10561 cgctgaatga atctgcgccc tgaattctgg taaaaaacat tatcgtaaat taccatttct
    10621 ttcaacagct tactagtaaa caagaagtta gcctccgtga atataaacgt cgccgaattg
    10681 ttaaatggga attacattct gttattattt gtggtcctcg cgcttgggct atgtctcggg
    10741 aaattacgac ttggttcgat ccaactgggt aattccattg gcgttttagt cgtatcgctg
    10801 ttattaggcc aacaacattt cagcattaac accgacgcgc ttaatcttgg ctttatgctg
    10861 tttattttct gcgtcggggt cgaagccgga ccgaactttt tttccatttt ttttcgcgat
    10921 gggaaaaatt acctaatgtt agcactggtg atggttggca gtgcgctggt catcgcctta
    10981 gggttaggta agctgtttgg ctgggatatt ggcctgacgg ccggtatgtt agcaggctct
    11041 atgacgtcaa caccggttct ggtcggtgct ggcgatacac tgcgtcattc cggcatggaa
    11101 agcaggcagc tctcattggc actggataat ctgagcctcg ggtatgcctt aacctattta
    11161 atcggcctgg tgagtttgat tgttggtgcg cgttacttgc cgaaattgca gcatcaggac
    11221 ttacagacca gcgcccagca aatcgcccgc gaacgtggcc tggacacaga tgccaaccgt
    11281 aaggtttatt tgccggtgat ccgcgcctac cgcgtcggcc cggagctggt agcctggacc
    11341 gacggcaaaa atctgcgtga actgggtatt tatcgacaaa ctggctgtta cattgaacgt
    11401 attcggcgta acgggattct ggcaaatcca gacggtgatg ccgtactaca aatgggcgat
    11461 gaaatagcgt tggtaggcta tcctgacgcc cacgcccgac tcgatcccag tttccgtaat
    11521 ggcaaagaag ttttcgatcg tgaccttctc gacatgcgta tcgtcactga agaagtggtc
    11581 gttaaaaacc ataacgctgt tggcaaacgc ctggcacaac tgaagttgac cgatcacggt
    11641 tgcttcctta accgcgtcat acgtagccag attgagatgc cgattgatga caacgtcgtg
    11701 cttaacaaag gtgacgtttt acaagtcagc ggcgatgccc gccgggtaaa aaccatcgcc
    11761 gatcgcatcg gctttatctc gattcacagc caggtcactg acttgctggc attctgcgcc
    11821 ttctttgtta ttgggctgat gatcgggatg atcaccttcc agttcagcac attcagtttc
    11881 ggtatgggga acgctgccgg gttgttattc gccggaatta tgctgggctt tatgcgtgct
    11941 aaccacccga ccttcggtta cattccgcag ggtgcattaa gcatggtgaa ggagttcggc
    12001 ttgatggtgt ttatggcagg cgttggtctg agcgctggta gcggtattaa taacggcctg
    12061 ggcgcgattg gcggtcagat gttgattgcc ggattgattg tcagtctggt gccagtggtt
    12121 atctgcttct tgttcggtgc ttatgtattg cgaatgaacc gcgcactgtt gttcggcgca
    12181 atgatgggcg cacgcacctg cgcgccggca atggagatca tcagtgatac agctcgcagt
    12241 aatatcccgg cactgggcta tgcgggcact tacgcaatcg ccaacgtcct gctgacgctg
    12301 gcagggacaa tcatcgtcat ggtatggcca ggattaggat aaaactgaag ttgccctgaa
    12361 aatgaaattt tttttgcaca accgcagaac ttttccgcag ggcatcagtc ttaattagtg
    12421 ccactgcttt tctttgatgt ccccattttg tggagcccat caaccccgcc atttcggttc
    12481 aaggttgatg ggttttttgt tgtctaaaat ttatctacta ccctgcaacc ctctcaacca
    12541 tcctcaaaat ctcctcgcgc gatagcggct tacggtcggt gacaaaatgc agcgtcatcc
    12601 cctcaataaa cgcatcaagc gcgcgggcgg ttccgggttc aaaccattgt tcgagcgttt
    12661 gctgactgcg ctgcatccag ttttgcatta ccgtttttaa tagcggtttt cggctagcca
    12721 gcgcgtagag ctggtacatc agctccatgt tatccggcgt tgcaacctgt gagctgtaaa
    12781 tcatatcggt gatagcctgg catgcgcccg gagcatcact aacatcgcta aaaaatgcct
    12841 gatattgccg ggacatgatc tcagtaaaac tgctgaacgc ctccagcaac aactcatcaa
    12901 ttcctgaaaa atagtaggtc atcgacccca acggtacccc ggcaagggta gcaattttgc
    12961 ggtgcgtaac agcatgtatt ccataaagtt tcaccgcctc cagcgtggcc tggataattt
    13021 tttctcgccg ttgcggatcg ttagcgcgac gcatgtcatt tcctctctct gcaattgtgt
    13081 acaaatgtac acaaccttgc taatgttgtc ttccctcttc ttattttgac gtgatctatg
    13141 accgtaaatt cttcacgtaa tgcattgaaa cgccgaacct gggcgctgtt tatgttcttc
    13201 tttttgccag gcctgttaat ggcgtcctgg gcaacccgta cgcctgctat ccgcgatatt
    13261 ctttctgttt cgatcgctga aatgggcgga gtcctctttg gtctgtcgat cggttcaatg
    13321 agcggtattc tctgctcggc gtggttagtg aaacgctttg ggacgcgtaa tgtcatcctg
    13381 gtcacgatgt cctgcgcatt gatcgggatg atgatactaa gtctggcact ctggctgaca
    13441 tcgcccctac tcttcgcggt tggtctcggc gtctttgggg caagttttgg ttctgcggaa
    13501 gtggcgataa acgttgaagg tgctgccgtt gagcgagaaa tgaataagac ggttttgccg
    13561 atgatgcacg gtttttatag cctgggcacg ctggcaggcg ctggtgtcgg gatggcacta
    13621 acggcctttg gcgttccggc aacggtgcac attttattgg cggcgctggt aggcatcgcg
    13681 cctatttata tcgccattca ggcaatccct gatggtacgg gcaaaaatgc tgccgatggc
    13741 acccagcatg gtgaaaaagg cgtacctttt tatcgcgata tccagttgct gctgattggt
    13801 gttgtggtgc tggcgatggc ctttgccgaa ggttctgcca acgactggtt acccttatta
    13861 atggttgacg gtcacggttt tagccctact tccggctcgc tgatttatgc cggttttacc
    13921 ctggggatga ccgttggacg ctttaccggc ggttggttca ttgaccgtta cagtcgcgtt
    13981 gccgtggttc gggccagtgc actaatgggg gcgttgggta ttgggctgat tatttttgtc
    14041 gatagcgcct gggttgctgg ggtgtctgtt gtactttggg gactgggtgc ctcgttgggc
    14101 ttcccgctga ccatttctgc cgccagcgat accggccccg atgcaccaac ccgcgtcagc
    14161 gtggtagcta cgaccggtta tctggctttc ctcgtcgggc cgccactgct gggctatctc
    14221 ggcgaacatt atggattacg tagtgcaatg ctggttgtac tggcgctggt tattctcgcg
    14281 gctattgtcg cgaaagccgt cgccaaaccc gataccaaaa cgcagacggc gatggagaat
    14341 agttgatgag cattaaatta attgcggtag acatggatgg tactttctta agcgatcaaa
    14401 aaacctataa ccgtgagcgg tttatggctc agtatcagca aatgaaagca caaggcattc
    14461 gctttgtggt cgccagcggg aatcaatatt atcagttgat ctctttcttt ccggaaattg
    14521 ctaatgaaat agcctttgtg gctgaaaacg gcggctgggt agtgagcgaa ggcaaagatg
    14581 tttttaatgg cgagctgtcg aaggatgcgt tttctactgt cgtggaacat ttgctgacgc
    14641 gcccggaagt ggaaattatt gcctgcggaa aaaatagcgc ctatacactc aaaaaatatg
    14701 acgatgccat gaaaacggtg gcggaaatgt attatcaccg tctggaatac gtcgataact
    14761 ttgacaactt agaagatatc ttctttaagt ttggtctgaa tctttccgat gaactgattc
    14821 cacaagtaca aaaagcatta catgaggcca tcggcgatat tatggtgccg gtccacaccg
    14881 gcaacggcag catcgatctg attatccccg gcgtacataa agccaatggc cttcgccaac
    14941 tgcagaaatt atggggaata gacgacagcg aagtggtggt ctttggcgat ggcggtaacg
    15001 atattgagat gctgcgtcag gcaggcttta gttttgcaat ggaaaatgcc ggcagcgcgg
    15061 tcgtcgcagc ggcaaaatac cgggcaggct ccaataaccg tgaaggcgta ctggatgtga
    15121 tcgataaagt gcttaaacac gaagcgccat ttaaccaata aaacggcagc acgcccggca
    15181 acacaatacc tgaatgaagg ctgcataatt cgcggaaatt aacccgctgc attatttcag
    15241 gcgaaatgat tatgaaaaat tgtctactgt tgggcgcgct tttaatgggc tttactggtg
    15301 tggcgatggc gcaaagtgtc accgtcgatg tgccgagtgg atacaaagtg gttgtggttc
    15361 ctgattcagt cagcgttcca caagcggtca gcgtcgcgac tgtgccgcaa accgtctatg
    15421 tggctcctgc gccagctccg gcttaccgcc cgcatcctta tgttcgccat cttgccagcg
    15481 taggtgaagg gatggttatc gaacaccaga ttgacgacca tcaccattaa ttcgagaatg
    15541 cctgatcgca caaagcaatc aggcattttt ttacccttcg tgcgaatttc ccatctgttt
    15601 atcttttaaa aagataacca tcagcaacag ccacaaaatt ccgttgacaa ggttgaagag
    15661 attaaacagt ccgttgcccc cgttcagcca gacatgtttg ctgatttcaa taccgacggt
    15721 aaagatcagc atttgcagca tccccatcgc cgcagaaacc gtacctttac tcatatcgct
    15781 ggcaaacagg gttaatcgca ccagtcccgc attcgccaga ccaataccga aagcataaat
    15841 acttaacccg gcggtcatcc ataaatacgc gtgcgatgag ataaccgttg ccgcagcagc
    15901 gaccaacaga ccaatcataa tcggccagcc gcccataata atcagcgaac gtacggtgcg
    15961 gcgcgaggtc agacgcgcta acagcaagtt acccgcaatt aacgccccga aaataggcac
    16021 ttgcagcaag ccatattcat agctgctcaa ctgctcaccg gtaataatga taatcggcga
    16081 ctgggcgatc cacgccaaca acggcaggct aacgaatccc agcgccagcg cccccgccac
    16141 aaagcggccg ttcttcagca ccagcttata gtcacgaccg agttctttca gcgacagttt
    16201 ctcgcctata cgcgtggcgg tttcaggcat ggctcgttgc agaccgaaaa aggagatcgc
    16261 tgccaatgcg gcaaacaaga caaacatccc ttcccagggc agcacatgga tccacgccgc
    16321 gcccaccagc ggaccaagta gtggagcaat cagcgccacg ttcgccatta gcgcggtgat
    16381 cttgatacaa accgcctctt cgaaggattc ctgaattgcg gcgtatccca cagcgccaat
    16441 gaaacagagg cttatgccct gcaagaagcg caacagggtg aattgttcaa tgttttgcgc
    16501 cagtaatatt gccagacagg tgacgataaa ccacaccact cccgccagca tcaccggacg
    16561 gcggccaata cgatccgaca acggccccag gagccattgt aaaaacatcc cgcccgccag
    16621 atacgcagtc atcgaagtag gaacccaatc aatgcccgcc tgatattgtt ccaccacggc
    16681 caacataccg ggttgaatca tatcgttgcc gatataggtt gaaaattcgt aaagcaccag
    16741 acagagaggg aaaagtaacg cctgacgtcc aagcctggca ccggaagcta atttattttg
    16801 catgcaattt cttcgccaat aataatcgcg caaagtttaa taaaagcgca gctaacgaga
    16861 aagcgaattt tgtagctgaa accacggtta agcacattct tacattattg cgagtatagc
    16921 tacgctttct ttaagtttta tttaaccttt gcccgttaca atcacccacc gtaaacaggc
    16981 cgcttgaggg aaataagacg atgccgcttt acccagttta acctgcactt tattctcaac
    17041 gacttgcctg tattggctcc cttttaatca ctttgcgtcg ggaagttatg ctggaaaatt
    17101 tgaatctctc tctattctct cttattaacg cgacgccaga ctctgcgccg tggatgatct
    17161 cgttggcgat ttttattgca aaagatttga ttaccgtggt gccgttgctg gccgcggtac
    17221 tttggctgtg ggggcttaca gcacaacggc aactggtgat aaaaatcgct atcgcgctgg
    17281 cggtcagcct gtttgtgtcc tggtcgatgg ggcatctttt tccgcacgac cgaccctttg
    17341 tcgaaaatat cggctataac ttcctgcatc atgcggcgga tgactcattc ccgagcgatc
    17401 acggtacggt gattttcacc tttgcactgg catttttatg ctggcatcgc ctgtggtccg
    17461 gctcactttt aatggtgctg gccgtcgtca ttgcctggtc gcgcgtttat cttggcgtcc
    17521 actggccgct ggatatgctc ggtggattgc tggcaggtat gattggctgc cttagcgccc
    17581 agattatctg gcaagcgatg gggcataaac tctatcaacg tctgcaatcg tggtatcgct
    17641 tctgttttgc attaccgatc cgcaaaggct gggtgcgtga ctgatctctg ctaaaaagtg
    17701 tagtattgag cggctcgctt caataactat tcagagggat tatggaaaca cgtcgcgaag
    17761 agcgtatcgg gcagctgctg caagaattaa aacgcagtga taagttacat cttaaagacg
    17821 ccgccgccct gcttggggtt tcggagatga cgattcgtcg cgatctgaac aaccacagtg
    17881 cgcccgtcgt tttgctcggc ggctatattg ttctggaacc acgcagcgcc agccattacc
    17941 tgttaagcga tcaaaaatcc cgcctggtgg aagaaaaacg ccgggcggca aaactggctg
    18001 cgacgctggt agaacccgat cagaccctct tttttgactg tggcaccacc acgccgtgga
    18061 ttattgaagc aattgataat gaaatccctt ttaccgccgt ttgttattcg ctaaatacct
    18121 ttctggcgct gaaagagaaa ccccattgcc gcgcgtttct ttgcggtggt gaatttcacg
    18181 ccagcaacgc cattttcaaa cccatcgatt ttcagcaaac gctgaataat ttttgcccgg
    18241 atatcgcttt ttattccgct gcgggcgtgc atgtcagtaa aggcgctacc tgctttaatc
    18301 ttgaagagtt gccggtaaaa cactgggcca tgtcgatggc gcaaaagcat gtgctggttg
    18361 tcgaccacag taaatttggc aaggtgcgtc cggcgcgcat gggtgacctg aaacgctttg
    18421 atattgttgt gagcgattgt tgcccggaag atgagtatgt gaagtacgcg cagacgcagc
    18481 gtattaagtt gatgtattaa tgacgtataa ccggatgacg tttcgcgcca tccggttatc
    18541 agaagattaa gagaaccagc tgccgaacca ctgatggaat ttcatcatca cgaaatccca
    18601 catccgacca aagaatccgc cctcttccac attttccatc acgatcagcg gacgctgctc
    18661 aatggattta ccgttaagct ggaaatcaat ggtgccgaca acctgacctt ttttcagcgg
    18721 tgcggtaagc tgcggttccg ttaacgtata actcgccttc aggtttttca gctgcccacg
    18781 cggtatggtc actgagcccg cttcgcctgc cccgagattc acttcgctct tatcaccaaa
    18841 ccagacgcgc tgagtcacaa atgtggcatc aggtttaatt ggcgtcacgg tttcaaagaa
    18901 acggaagccc caggtcaata atttctcaga ctcattaaaa cggatacggt cagttttcgc
    18961 ccccagcact acggagatta aacgcatatc gccctgggta gccgaagcaa ccagattata
    19021 tcccgcgcct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
    19081 cagacgatta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
    19141 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
    19201 ggtactgaac tgccccggcg catccagacc gtgcaccgtc tggaaggtag tgttggtcag
    19261 acccagtttt ttggcataac cattcatcag accaataaat gactcctggc tcccggcaac
    19321 gtaatcagcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
    19381 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
    19441 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
    19501 atcggcctta agcgcctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
    19561 gtccagtttt tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
    19621 aatccatgca cgcgcatcca cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt
    19681 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga ccacgaagga gagaggagta
    19741 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaaa tacgttatat cgccgtatga
    19801 taagttcggt gattaataac gcacacacaa acttaaagaa accgtaagtt ggtaaagttt
    19861 ttaaagttta tgcaatatcc tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg
    19921 ttgcttaaaa gttaacttta cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc
    19981 ggaaaagggg tgagtatgat tacgctgtgg ggtcggaata attcaaccaa cgtaaaaaaa
    20041 gtattgctga cgctcgaaga actggaacta ccttatgagc aaattctcgc gggccgtgag
    20101 tttgggataa atcacgatgc tgattttctg gcgatgaacc ctaacgggct ggtgccgttg
    20161 ttgcgtgacg acgaaagtga tctcattctt tgggaatcaa acgccattgt ccgctatctg
    20221 gcggcacagt acgggcaaaa acgcctgtgg atcgactcac cggcacgtcg tgcggaagcc
    20281 gaaaaatgga tggactgggc aaaccagacg ctcagtaatg ctcatcgcgg gatcctgatg
    20341 ggattagtca gaacaccacc ggaagagcgc gatcaggccg ccattgatgc cagttgcaaa
    20401 gagtgcgacg ccctgtttgc cctgctcgat gcagaactgg caaaagtaaa atggttctca
    20461 ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt tcatctacaa tttgtttaac
    20521 gttggcctga cctggacacc gcgtccaaat ctgcaacgct ggtatcagca actcactgaa
    20581 cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct aattgcgtgg gctaacttta
    20641 agtaattccc cactggactc gtcggtgaga acgtataaat aaccgtcggg tccagtgcga
    20701 acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc catcttctgt cactttgtcg
    20761 ccgttgacgc tcatcacaat gacatcttta tctttcagcg cgccaataaa taatttttgc
    20821 tgccactggg ggaatttgtc gctgttatag aaggccatgc cgctcacggc gggcgaatct
    20881 ttccagtaaa aaacaggttg ctcggtcccg gcgacgatct cccctttcgc ctccggtatc
    20941 ttaaagcctg aatagttgat tccccaggtt gccagcggcc agccgtagtt tttgcctttt
    21001 tgcgggatat taatttcatc gccaccgcgc gggccatgtt cattcagcca tagtgcatta
    21061 ctccacggat tcatcgccat tccttgcgga ttacgaatgc cataagacca gatctcggcg
    21121 cgtgcatcgg attcctttat aaaaggatta tcatccggga tttctcccag gtcggtcagt
    21181 cgcaccagtt tgccctgtaa tttatccaga tcctgcgccg tcgggcgctg attgttttcg
    21241 cccagagcaa taaaaagata acctttaccg tcaaatacca tccgcccgcc aaaatggttg
    21301 ccggtagaca gttttggcat ctgacgaaag acggtgcgga agtcggtcac ttttgagaga
    21361 tcatcactta agcggccata acccacagca gttccggctt tgccatcatc gccaacttcg
    21421 gaataactta accagatgcg gcgcgactga gcaaaatcgg gcgctaaaac cacgtccagc
    21481 aggccgccct gcccgtgcgc ccaaacgtcc ggcactccgg aaagcggcgc agataatcct
    21541 tttcctgctt gccagtgacg caactcgccg ccgcgcagag tgattaacat accgtgattg
    21601 tcgggtaaaa aggccagcgc ccagggatgg tcgagtttgt cctgcagtac ttcgacattt
    21661 accgttgcag gagccgccca gagagcggaa gaaagacaaa taaggggcac aaggaaaaag
    21721 gattgtcgat gcataacgcc tccggattga ctctggctta aagcgtagtc agtggaggag
    21781 ataacaaatt tatttttaca aaaacttaaa catgaagggg gagacgcttt ctccccctta
    21841 gttttcaggc cttctcaagc atggcgtgct tctgcaggct ctggatactc agcgttaagc
    21901 tcatcagaca attttcaagc ttatcggcgt tgacggtaat aacagtcggg caatcatggt
    21961 gcccactcat caaacatact gcggctgtcg ctaatgcttc ttcagcatga tgaagagcac
    22021 tccactcttc ctgatccaga tgaagattca accgcagcga tttatcgtgc agttcgcgat
    22081 tcagtttaaa aaagttatct cgtagatgat tgctttcgct gacggacatg tatccttttg
    22141 ccttcctcag ttgcacgtat gcggcgaggt caacgcgtgc gtcgatcaac cggttcagca
    22201 gatcgattcg ctgtctgtca acgaacatac ttcgatcctc ctctttccag ccttgttgca
    22261 attaaatagt atggctattt tttgagcaaa atgctgtcta ggatcaattc ttcacgccga
    22321 attaacaaaa tttacatttc cttcgcctcc gccagcgaat gagattgcga tacaaatttt
    22381 atccagaact ctgtagaatc cctgccctga cctttactgt aactgaatat tttttaagct
    22441 atgagcaaag taactcccca gccgaaaatc ggctttgttt cccttggctg cccgaaaaac
    22501 cttgtcgatt cagagcgtat tctcaccgaa ctacgcactg aaggttatga cgtggtaccg
    22561 agctatgacg atgcggacat ggtgatcgtc aacacctgcg gctttattga cagcgcggta
    22621 caagaatcac tggaagccat tggtgaagcg ttgaatgaaa acggcaaggt aattgtgacc
    22681 ggttgtctgg gggcaaaaga agatcagatc cgcgaagtcc acccgaaagt gctggaaatc
    22741 accgggcctc atagctatga gcaggttctg gagcacgttc atcactacgt gccaaaaccg
    22801 aaacacaacc cattcctgag cctggtgcca gaacaaggtg tgaaactgac gccgcgtcat
    22861 tatgcctatc tgaaaatttc tgaaggctgt aatcaccgct gcaccttctg cattattccg
    22921 tctatgcgcg gcgacctggt gagccgtccg attggcgaag tgttaagtga agcgaaacgt
    22981 ctggttgatg cgggcgttaa agagattctg gtgatctcgc aggatacttc cgcctatggc
    23041 gtcgatgtta aacatcgtac tggcttccac aacggcgagc cggtaaaaac cagcatggtc
    23101 agcctgtgcg aacagttatc gaaactgggg atctggacac gtctgcacta cgtttaccct
    23161 tatccgcatg tggacgacgt catcccactg atggcagaag gcaaaatcct gccctatctg
    23221 gacattccgt tgcagcacgc cagcccgcgc attctcaaac tgatgaagcg tccgggttct
    23281 gtagatcgcc aactcgcgcg catcaaacag tggcgcgaaa tctgcccgga actgacccta
    23341 cgctcaacct ttattgtcgg cttccctggc gagacggaag aagatttcca gatgctactc
    23401 gacttcctga aagaagcacg tctggatcgc gttggctgct ttaaatacag cccggttgaa
    23461 ggtgcagacg ccaatgccct gcctgaccag gttccggaag aagtgaaaga agaacgctgg
    23521 aaccgtttca tgcagttgca gcagcagatt tccgccgagc gcctgcaaga gaaagtgggc
    23581 cgtgaaattc tggtgattat cgacgaagtg gacgaagaag gtgcgattgg tcgcagcatg
    23641 gcagatgcac cggaaatcga cggcgcggtt tatctcaatg gtgaaaccaa cgttaagccg
    23701 ggtgatatcc tgcgtgtgaa agtcgagcac gccgatgagt acgatttgtg gggtagccgg
    23761 gtttaattaa tcttaaggca gatctaacaa ggatattgtt cttcgatctg cctctgctac
    23821 ccttcccgat gtcctaccct tacgcacaaa atcccctgac gcataaatcg agaaaaatga
    23881 atcgtactcg aggcaactct cactttttca caccaaatta aagttttgat cgcaattact
    23941 tgaaataagc gtaatggtta ctatagtaaa cattaccaca aggatggatg tatgggtgtg
    24001 tataaggcaa gacgattcag ccaaagcacg aaaaaacttg gtattcacga taaagttctc
    24061 atggctgccg ctgaagaagt gattcagggc atatgggagg ctgatttagg tagcggtgtc
    24121 atcaaaaaac gtttacccct ccaacaagga aagagtggtg gagccagaac aattatcttt
    24181 tttaaatcag caaatcatgt gtttttttat gatggttggt ccaagagtgg tctaagcagt
    24241 aaaggaacca aagaaatcga agatgatgaa ctggcggcct acaaaaaaat ggcaaatgca
    24301 tttctggcct ttagcaataa gcaaatagag gatttgatcg aaactggctt tttgattgag
    24361 gtgaaaaatg agcgataaat tacaaatgat gctggaagat gcaaaggaac tgaatgcact
    24421 gggcctgatg tctgatagtg aagttgatgc cattgccgtc atggtcaaag ctcgtgaagt
    24481 cagcgcccgt atagccaaag tcaaatcaat gacgggtgaa gagatcaaag ccatccgcac
    24541 ccgatacggc atgtcacaat ccgtgctggc acatacaatg ggaatgtcaa aagaaagtgt
    24601 ctcgaaatgg gaacgtaatg aaataaaacc gagcggcccg gcattacgta ttttgaatac
    24661 attggcaata aaagggccgg aagtttttgc aaagtaaaat gtttgcaaat gatttaccac
    24721 attcattagt accccttgat taaggggtac taacactatt ctaattctac tgccagctaa
    24781 tctgcgccct gccgtttttc ttactcttat acagtcgctc atccgccagc attttcaagc
    24841 gttcgacggt agtacattca tttgaatgcg ctacaccgat actggtgctt aaattaatgc
    24901 ttcccacctg gaaatgaaca atttctttta gaatcgatag caactcttcc agcgttccac
    24961 ccgtataaat caccgcaaat tcgtcaccgc cgaagcggta gactttacct ttatctacgg
    25021 ggagctgtga tttaaggatt ttcaccactt tcatcaacac ttcatcgccc agcaaatgac
    25081 cgaaggtgtc gttaatgttt ttgaatttat cgacgtcaaa caggcacaaa taaccgcctt
    25141 tttccggcag cgcttttaaa tcttcgtcaa aggattttct gcccatcgct ttggtcagat
    25201 cgtccaggaa tgccgagtta tacatactgt agagtttttt gacatcggca gccgaaaaga
    25261 aaagcccgcc atccagtacg cgttcaaaat ggttattaat aatggcttct ttctccacaa
    25321 taccacgatt aatcactctg cgtaccagat agaggcaact ggcacccgat atcactgcca
    25381 ccaatgcggc aaagaaaata atcaatgtac atacctgaaa ataaacgtct tttttataag
    25441 caaatacgta caacccggtc ggcaaatagg cgaagtgctc catcgcttcc gaaacagtga
    25501 agatggcgtt tttagcgtta tattcatcac cggtcaccga tttaccgttc ttgaacaggg
    25561 agaacatcag gttctcgcta tccatcgcca gcgattttga cagtaaagaa aaacgttcca
    25621 gaccaagcaa aaatatcaga ctacgattgt gagttaccgg atagaggtaa tagacgaagg
    25681 ttttaccatc aaaagagata aaccctgtat ttttgacatg tcgatacttc acaacctgca
    25741 taatatctgc acaataattt ttctcgaact cctttacctg aatacattcg tcactggaaa
    25801 tcagataagg accgacatcg tacaaataag agggtgaaac cgagtcggcg taagctgtgc
    25861 gtaagccaac caacagctgt tcctccgtgg gatttagcgt gttgataaga tcaattgcca
    25921 cgctacgccg atattcagca atatttgcca ccgcaaaagc agtatcaata acggactgtt
    25981 tcaggctata cacccgttcc tttaccattt gagtgtagat cccgcaggca actgctgaaa
    26041 tcatgataaa aatcagcaga ctgactgtaa gtacgaactt attgattctg gacatacgta
    26101 aactactctt ttactaattt tccactttta tcccaggcgg agaatggcat gggtttctgg
    26161 tacagatacc cctgagcctg aacacatcct aatttgatta attcgcgcgc aacgtcttcg
    26221 ctctctacgc cttccgcaat caccgggatc cggttatagc gtgccagatc gaaaataatg
    26281 cgcacaattt ttcccgcagt accaaaaata tcgccggtaa actgcgagtc cacctttatg
    26341 ctgtctggat taatttcata aaaacgtttc aggtttgaca aaccggtgcc aaagtcatca
    26401 atcgatatcc gtaccccttg tttacgcagt tgttcaatat tgagataaaa atgttcatta
    26461 attcgctcaa tcacggtttc agtgatctca acacaaaggc tgttcgcctt caggccattt
    26521 tctttcatga tggtagtaaa acgctcatga aagtgtggtt cgtttaactg ccagggcgaa
    26581 acattaatat gcagtaagaa atcatccttc agcgacgcac gctgactaaa atgtctgaat
    26641 tcacccatcg cggttttaat aatttgatag cccagctcat tgataaaccc gatatcttca
    26701 gcaatggtaa tgaacttcag cggtgaaatg atcccccgct ccgcagacac ccaacggcac
    26761 aacgcctccg cgcccagaat ggcgcgggtg ttaatatcaa cgatcggttg atagacgagg
    26821 aatagttcgc caatttccat tgcatggcga atattacgcg caatccagat attctcttct
    26881 atttcgttga gtttcgcatc atcacagaaa ataaactctg agaacatatg gttgcggata
    26941 ctggaaatcg ccagtttcag cttttctgcg taactccagg cgttttcccc ctgcaaaggt
    27001 tcacaaatag cagacttatg gatgcgcaga ttgcggtagc caaaactgct gtcaaaatcg
    27061 tttaccgtcg ctatcttacg ggtaaacgtc tgtacatttt cccggcacag gagtaaatag
    27121 agatcggcat taaatgtcac gatatcgcag cattgcgcat atttctcggc aattcgctga
    27181 cacaatttcc tgacgatttt attgcgttca tcaacaccaa acaccgcttc cacagcttcc
    27241 agaccacaca gttagatgat ccccacagcc agatactgat tattgttgga ttcaataatc
    27301 gccatatttt taataaaacc gctttcctga tcgtaagaga tcagtgagag caatttattt
    27361 gaccgttcaa tttcggcatc tttcatttcc gcgatcgaat ttttgatgct gataatctct
    27421 ttcgcatcgc cttcgcgaat tttcagagcc tcgatctgat ccgttgagtc gggcaaaaac
    27481 gccagcgtat taaccagctc ggtcatttca gaaaattgcc gacggaacac acgagtggtc
    27541 attaaataaa ccaccaacaa tacgataata atgaacgcaa acggcagata aaaaagttta
    27601 tctgatgcgt tcaataaatt accaccagga taataaaaat agatttcgaa taatgcgttg
    27661 tttggcaccg ctgatgtttt ttcaatatat tccgtatcga cagaatcaat aatattataa
    27721 ttaccttcgc tattataaat aatcaatttt tcacgcgcga gttgattctt ggcaatgatc
    27781 tcatgacctt gctttaaaaa tagaacttca tcattagcgc tgatgtgatc aagatactcg
    27841 ttcagatatc gccagtttaa atcgacatga attttatcca ccactaacaa atcgtgatct
    27901 ttgccgttga tgcgtagcgt aaaagagata cattttttac ccggacgatc aaagcaattg
    27961 tatgccggat tccagctgtt gttagagtca tctgaagcca tcgaccacgg tcgtttcgat
    28021 gggttaaaat cggcaaaaat aacccgccct gactcgtcga caagcggtcc atttacttgt
    28081 tcctgatgat aaacaacgat tttatcattc gccctccgcg aaaag
//
LOCUS       DAZGHF010000057        28083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGHF010000057 DAZGHF010000000
VERSION     DAZGHF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.057"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            136..807
                     /locus_tag="ACHK3A_002521"
     CDS             136..807
                     /locus_tag="ACHK3A_002521"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO8740583.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDIEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETPSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            817..2013
                     /gene="yaiP"
                     /locus_tag="ACHK3A_002522"
     CDS             817..2013
                     /gene="yaiP"
                     /locus_tag="ACHK3A_002522"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO8740584.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            2048..2572
                     /locus_tag="ACHK3A_002523"
     CDS             2048..2572
                     /locus_tag="ACHK3A_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO8740585.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPT
                     GTYSLRQELIRTGD"
     gene            2574..3347
                     /gene="yaiO"
                     /locus_tag="ACHK3A_002524"
     CDS             2574..3347
                     /gene="yaiO"
                     /locus_tag="ACHK3A_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO8740586.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3532..3807
                     /gene="frmR"
                     /locus_tag="ACHK3A_002525"
     CDS             3532..3807
                     /gene="frmR"
                     /locus_tag="ACHK3A_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO8740587.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3842..4951
                     /gene="frmA"
                     /locus_tag="ACHK3A_002526"
     CDS             3842..4951
                     /gene="frmA"
                     /locus_tag="ACHK3A_002526"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO8740588.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5043..5876
                     /gene="fghA"
                     /locus_tag="ACHK3A_002527"
     CDS             5043..5876
                     /gene="fghA"
                     /locus_tag="ACHK3A_002527"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO8740589.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFAPIVSPSQVPWGQQAFTAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6103..6642)
                     /gene="yaiL"
                     /locus_tag="ACHK3A_002528"
     CDS             complement(6103..6642)
                     /gene="yaiL"
                     /locus_tag="ACHK3A_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO8740590.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDYLMW"
     gene            complement(6744..7955)
                     /gene="mhpT"
                     /locus_tag="ACHK3A_002529"
     CDS             complement(6744..7955)
                     /gene="mhpT"
                     /locus_tag="ACHK3A_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO8740591.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGIGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8131..9144)
                     /gene="mhpE"
                     /locus_tag="ACHK3A_002530"
     CDS             complement(8131..9144)
                     /gene="mhpE"
                     /locus_tag="ACHK3A_002530"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO8740592.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9141..10091)
                     /gene="mhpF"
                     /locus_tag="ACHK3A_002531"
     CDS             complement(9141..10091)
                     /gene="mhpF"
                     /locus_tag="ACHK3A_002531"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO8740593.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10088..10897)
                     /gene="mhpD"
                     /locus_tag="ACHK3A_002532"
     CDS             complement(10088..10897)
                     /gene="mhpD"
                     /locus_tag="ACHK3A_002532"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO8740594.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10907..11773)
                     /gene="mhpC"
                     /locus_tag="ACHK3A_002533"
     CDS             complement(10907..11773)
                     /gene="mhpC"
                     /locus_tag="ACHK3A_002533"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO8740595.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVKAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11791..12735)
                     /gene="mhpB"
                     /locus_tag="ACHK3A_002534"
     CDS             complement(11791..12735)
                     /gene="mhpB"
                     /locus_tag="ACHK3A_002534"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO8740596.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12737..14401)
                     /gene="mhpA"
                     /locus_tag="ACHK3A_002535"
     CDS             complement(12737..14401)
                     /gene="mhpA"
                     /locus_tag="ACHK3A_002535"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO8740597.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14592..15425
                     /gene="mhpR"
                     /locus_tag="ACHK3A_002536"
     CDS             14592..15425
                     /gene="mhpR"
                     /locus_tag="ACHK3A_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO8740598.1"
                     /translation="MQNNEQTAYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15502..16584
                     /gene="lacI"
                     /locus_tag="ACHK3A_002537"
     CDS             15502..16584
                     /gene="lacI"
                     /locus_tag="ACHK3A_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO8740599.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPQALADSLMQLARQISRLESGQ"
     gene            16707..19781
                     /gene="lacZ"
                     /locus_tag="ACHK3A_002538"
     CDS             16707..19781
                     /gene="lacZ"
                     /locus_tag="ACHK3A_002538"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO8740600.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSKEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDISLLHK
                     PTTQISDFHVTTRFNDDFSRAVLEAEVQVYGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVLRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLTENLSVTLPSAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQTEVALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19833..21086
                     /gene="lacY"
                     /locus_tag="ACHK3A_002539"
     CDS             19833..21086
                     /gene="lacY"
                     /locus_tag="ACHK3A_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO8740601.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21152..21763
                     /gene="lacA"
                     /locus_tag="ACHK3A_002540"
     CDS             21152..21763
                     /gene="lacA"
                     /locus_tag="ACHK3A_002540"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO8740602.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVHFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKHYYYKDYKVESSV"
     gene            complement(21866..23047)
                     /gene="cynX"
                     /locus_tag="ACHK3A_002541"
     CDS             complement(21866..23047)
                     /gene="cynX"
                     /locus_tag="ACHK3A_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO8740603.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQNAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVFWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(23053..23523)
                     /gene="cynS"
                     /locus_tag="ACHK3A_002542"
     CDS             complement(23053..23523)
                     /gene="cynS"
                     /locus_tag="ACHK3A_002542"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO8740604.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23554..24213)
                     /gene="cynT"
                     /locus_tag="ACHK3A_002543"
     CDS             complement(23554..24213)
                     /gene="cynT"
                     /locus_tag="ACHK3A_002543"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO8740605.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTSIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24323..25222
                     /gene="cynR"
                     /locus_tag="ACHK3A_002544"
     CDS             24323..25222
                     /gene="cynR"
                     /locus_tag="ACHK3A_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO8740606.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(25224..25315)
                     /locus_tag="ACHK3A_002545"
                     /pseudo
     CDS             complement(25224..25315)
                     /locus_tag="ACHK3A_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25355..26638)
                     /gene="codA"
                     /locus_tag="ACHK3A_002546"
     CDS             complement(25355..26638)
                     /gene="codA"
                     /locus_tag="ACHK3A_002546"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO8740607.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLLDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26628..27887)
                     /gene="codB"
                     /locus_tag="ACHK3A_002547"
     CDS             complement(26628..27887)
                     /gene="codB"
                     /locus_tag="ACHK3A_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO8740608.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         6687 a         7642 c         7308 g         6446 t
ORIGIN      
        1 tacctggcag ccccttgttt ttcagctatc agctattttt aacacttaaa aattagcatc
       61 tgtctgcgtc agaggttacg tctctgcggg cgtcgttgtg cttgtagtat aataaataaa
      121 atggagtgtc ataaagtgga taaggtttta gattcagcga tcctttcttc ggcaaataaa
      181 agaaagggta tccttgccat tggcgcgcat cctgacgata tagaattagg ctgtggcgca
      241 tcgctagctc gccttgcgca aaaaggaatt tatattgcta ccgtggtgat gactaccggt
      301 aattctggcg tagatggaat aatcgatcgc cacgaagaat cacgcaacgc tctaaagata
      361 ttagggtgcc accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc
      421 aatgatatga tttctgccct ggaaaatatc ataaaaaatc aaattccctc tgatattgaa
      481 attatacgtg tatataccat gcatgatgct gaccgccatc aggatcatct tgcagtctat
      541 caagcctcaa tggttgcctg tcgcgctatt ccgcaaattc tcggctatga aacaccaagt
      601 acctggcttt catttatgcc tcaggttttt gaatccgtga aagaagaata tttttctctt
      661 aaactgactg cgttaaaaaa acataaaagt cagagccagc gagattatat gcgcccggag
      721 cgtctgcgtg cagttgctca atttcgcggg caacaggtca atagcgatct gggtgaaggt
      781 tttgttattc ataaaatgat tctttgagca aattgcatga aaacctggat ctttatatgt
      841 atggccgtag caatattgct atggtttctg agtacgttaa ggcgtaagcc cagccaaaag
      901 aaaggctgta ttgacgccat tatacctgct tataacgaag gcccgtgtct ggcgcagtca
      961 ctggataatc tgctgcgtaa cccttatttc tgccgggtaa tttgcgttaa cgacggctcc
     1021 acggacaata ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttatt
     1081 gccgtcacgc aaaaaaatac cggtaaaggt ggtgcgctaa tgaatggcct caattacgcc
     1141 acctgcgatc aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga
     1201 atgggctata tgctggcaga aatagagcgc ggtgctgatg ccgtaggcgg cattccctct
     1261 actgcgttga aaggcgcggg tctgttaccg cacatccgcg cgaccgtaaa gttgccgatg
     1321 attgttatga agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt
     1381 gcctgcggga tgttccgtac tgatgtattg cgtaagttcg gtttctctga tcgtactaaa
     1441 gtcgaagacc ttgatctcac ctggacattg gtggcaaacg gctatcgtat tcggcaggcg
     1501 aatcgctgca tcgtataccc acaggaatgc aacagcccgc gtgaggaatg gcgtcgctgg
     1561 cggcgttgga ttgtgggcta cgcggtctgt atgcgcctgc ataaaagact tttatttagc
     1621 cgcttcggta tcttcagtat atttcctatg ctgttggttg tgctttatgg cgttgggatt
     1681 tatctcacta cctggtttaa tgaattcatc accaccgggc cacatggtgt ggtgttggca
     1741 atgttcccgc ttatctgggt cggcgtagtt tgtgttattg gtgcatttag cgcctggttt
     1801 catcgttgct ggttgttggt gcctttagca ccgctttccg ttgtgtatgt attattagct
     1861 tatgccatct ggattattta tggacttatt gcctttttta ctggacgcga acctcagcgc
     1921 gacaaaccca cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccacccttct
     1981 gtcacaggaa ctgaaaaact atctgaagct taatgttaaa gagaaaaata ttcagattgc
     2041 agaccaggtg attattgatg aaagtgccgg tgaggttgtt atcggcgcga atacccgtat
     2101 ttgccacggt gccgttattc agggtccggt agtgattggc gcaaactgcc tgataggtaa
     2161 ttatgcgttt attcgtcctg gcacaataat cagcaatggc gtaaaaattg gttttgccac
     2221 cgaaattaaa aatgcggtta ttgaagcgga agcaacgatt ggtccgcaat gctttattgc
     2281 cgactcggta gttgcaaacc aggcatattt gggcgcacaa gtacgaacca gtaatcatcg
     2341 tctggatgaa cagcccgtgt ctgttcgaac tccagaggga attatcgcta ccggatgcga
     2401 taaattaggt tgttatatcg ggcagcgttc acgccttggc gtacaagtta ttattttgcc
     2461 tgggcgaatt atttccccga acacgcaact tggcccgcgc gtgattgtag aacgtaattt
     2521 acctaccgga acttattcac tccgacaaga acttatccgt acaggagatt aaaatgataa
     2581 aacggacgtt attagcggcg gcaatattta gcacattgcc cgcttatgcc gggttaactt
     2641 ccattaccgc gggctacgat tttaccgatt attctggcga tcatggcaac cgtaatttag
     2701 cgtatgctga actggtggcg aaagttgaaa acgcaacgct gctttttaat ctttcccagg
     2761 ggcgtcgtga ttatgaaact gaacatttca atgccactcg cggtcagggt gcggtctggt
     2821 ataaatggaa taactggctg acaacccgaa cgggtattgc ctttgcggat aatacgccgg
     2881 tctttgcccg ccaggatttt cgtcaggata ttaacctggc cctgttgcca aaaacgcttt
     2941 tcacgaccgg ttatcgctac actaaatatt acgatgatgt cgaagtcgat gcctggcaag
     3001 gcggcgtatc cctctataca ggcccggtga tcaccagcta tcgctatacc cattatgact
     3061 ccagcgatgc aggtggtagt tacagcaata tgatttccgt gcgtctgaat gacccgcgcg
     3121 gcactggtta tacgcaacta tggctaagcc gcggaacagg tgcttacacc tatgactgga
     3181 cgccagaaac acgctacggc agcatgaaga gcgtcagttt gcaacgtatt caaccgctga
     3241 ctgagcaact taatctcggg ctgacggcag gtaaagtgtg gtacgacacc ccaaccgatg
     3301 attataacgg tctgcaactt gcagcccatc tgacctggaa attctgattc cttctgccgc
     3361 ccgctatccg gggcggcctt ccctgtcgat taggcccctc tttacttttt gttatccgac
     3421 tacattcacc ggataaattt tattctccag tgttatatac tatagagggg tatgcattga
     3481 catataacat acccccctat agtatattgc gtgcagatga tgaggtgcga aatgcccagt
     3541 actccggaag agaagaaaaa ggtccttact cgagttcgtc gtattcgggg gcagattgat
     3601 gctctggaac ggtcgctgga gggtgatgcc gaatgccgtg ccatactcca acagatcgct
     3661 gccgttcggg gcgcggctaa cggactgatg gcagaagtgc ttgaaagcca tatccgggaa
     3721 acgtttgacc gaaatgactg ctacagccgc gaagtcagcc aatccgttga cgacactatt
     3781 gaactggttc gtgcctatct taaatagctg aatctattac catattgagg aagagcgaga
     3841 gatgaaatca cgtgctgccg ttgcatttgc tcccggtaaa ccgctggaaa tcgttgaaat
     3901 tgacgttgca ccaccgaaaa aaggtgaagt gctgattaaa gtcacccata ccggtgtttg
     3961 ccataccgac gcatttaccc tctccggcga tgacccggaa ggtgtcttcc cggtggttct
     4021 cggtcacgaa ggggccgggg ttgtagttga agtcggtgaa ggcgtaacca gcgtcaaacc
     4081 tggcgaccat gtgatcccgc tttacaccgc agagtgcggc gagtgtgagt tctgtcgttc
     4141 tggcaaaacc aacctgtgtg tcgcggttcg cgaaacgcag ggcaaaggct tgatgccaga
     4201 cggcaccacc cgtttttctt acaacgggca gccgctttat cactacatgg gatgctcaac
     4261 attcagtgaa tacaccgtgg tcgcggaagt gtctctggcc aaaattaatc cagaagcaaa
     4321 ccatgaacac gtctgcctgc tgggctgtgg cgtgaccacc ggtattggcg cggtacacaa
     4381 cacagctaaa gtccagccag gtgattctgt tgccgtgttt ggtcttggcg cgattggtct
     4441 ggcagtggtt cagggcgcgc gtcaggcgaa agcgggacgg attatcgcta tcgataccaa
     4501 cccgaagaaa ttcgatctgg cgcgtcgctt cggtgccacc gactgcatta acccgaatga
     4561 ctacgacaaa ccgattaaag atgtcctgct ggatatcaac aaatggggta tcgaccatac
     4621 ctttgaatgc atcggtaacg tcaacgtgat gcgtgcggcg ctggaaagtg cgcaccgcgg
     4681 ctggggtcag tcggtgatca tcggggtagc tggttctggt caggaaatct ccacccgtcc
     4741 attccagttg gtcaccggtc gcgtatggaa aggttccgcg tttggcggcg tgaaaggtcg
     4801 ttcccagtta ccgggtatgg ttgaagatgc gatgaaaggc gatatcgatc tggaaccgtt
     4861 tgtcacgcat accatgagcc tggatgaaat taatgacgcc tttgacctga tgcatgaagg
     4921 caaatccatt cgaaccgtaa ttcgttactg atttcccgca ggtttacccg tccagttcag
     4981 acggggttct taatactctc cccgggcagc cgtccggggg attaaccgtg agataatgac
     5041 tgatggaact cattgaaaaa catgccagct ttggcggctg gcaaaatgtg tatcggcatt
     5101 attccccatc actgaaatgt gaaatgaatg tcggcgtcta tctcccgcca aaagccgcaa
     5161 atgaaaaatt gccggtgttg tactggcttt caggcctgac ctgcaacgag cagaatttca
     5221 ttactaaatc ggggatgcag cgttacgcgg ctgagcacaa cattattgtt gttgcgccag
     5281 acaccagtcc gcgaggcagt catgtcgcag atgccgaccg ttacgatctc gggcaaggtg
     5341 ccgggtttta cctgaacgca acgcaagcgc cgtggaatga acattacaaa atgtatgact
     5401 atatccgcaa cgagctgccg gatttagtga tgcaacattt tccggcaacg accagaaagt
     5461 ctatctcagg tcattctatg ggcgggctgg gcgcgctggt gctggcgtta cgtaacccag
     5521 atgaatatgt cagcgtctcg gcgtttgcgc ccattgtctc cccatcgcaa gtaccgtggg
     5581 gacagcaagc ctttaccgca tatcttggtg aaaataaaga tgcctggttg gattacgatc
     5641 cggtgagtct tatttcacaa ggtcaacgcg ttgcggaaat aatggttgat caggggttga
     5701 gtgatgattt ttacgcagaa cagctgcgga ctccaaatct tgaaaagatc tgccaggaga
     5761 tgaatatcaa aacgttaatc cgttatcacg agggttatga tcacagctat tattttgtct
     5821 ccagttttat tggcgagcat attgcgtacc acgccaataa actgaatatg cgttgagcat
     5881 ccgcgcacgc atgccggatg cggcgtaaac gccttatccg gcctacattt cacccgtaag
     5941 ccgtaggcct gataagacgc gaaaagcgtc gcatcaggca tctgcgcacg gctgccggat
     6001 gcggcgtgaa cgccttatcc ggcctacact tcgcccgtaa accgtaggcc tgataagacg
     6061 cgtaagcgtc gcatcaggca tcactgcacg gcttatctat gtttaccaca tcaaataatc
     6121 aggcactttg aagtcggcat acggatcgtc ttcatcctgc tcttcggcgc tgagcgcgct
     6181 gtgcaacaca atactgctgg catcacgctg agcaatttta tcggcaacac tggcgggaat
     6241 aatggcgtat tcaccttcgc tattgttatc gaccaacaag cgggcaatcg ccagacgacc
     6301 attaatcaac tgcgcctgtg tgagcttatc gacaaaaatc ttcttaatca gattgccgtc
     6361 agtgaagtta aaaccaatat cgccattggc aatggtgatt cggttcattt caatgagctg
     6421 cttcacctgg gctttatatt ctttcgccaa cgccgcttgt ttttgctgtt cgctaagctg
     6481 tttatcacgc tcaagctgtg cctttttatt ttcttctacc gccgcccgag cttcacgcgc
     6541 ctgaacgcgc gattttttcg ccgttctctc gaccttcgcc gcttttttgc tggtgactaa
     6601 tcctgctttg agcaactgct cttgtaaggt aagttttgcc atctttgttt ccaaacccgc
     6661 tgaataattt ttagcgagta tacctgtaaa cgccgtaggt gtattggggg caagtccctt
     6721 tcgcccctgc ttaataaggc gcgtcaggca tcggcgcacg gctgcattcg tgatctccgg
     6781 ctcatcaaaa taaatactgc caggccagca acaagaatac ccggcgcaga agcggccatt
     6841 acgccaaccg tgccaatgcc taatgccagc attttcccgg ccagtaacgg accgctcata
     6901 gcccccagac gccctaccgc cacggctgtt cccacacctg ttgcgcggat ctgcgaactg
     6961 taaaacaacg gtgccagggc atacaaaacg ctttgcccac ctgtcgcaaa caaccccgcg
     7021 acgaatcccg ccagcaacat accgttaaat gacgacaccg ttcccagcgc cagcagcgaa
     7081 gctaacatgc cgctataaat cagtagcgac atggttactg gacgcagctt atccatcaat
     7141 gcgcccaaca ttaacgtccc gcttgccgcc cccatttgca gtgcaaacat cacccctgcc
     7201 gcctgcgatg gctggaatcc ttgctccacc aaaagcagcg gtagccagtt gatcaacatg
     7261 tagaccacca gcagagtgaa gaaataacac aaccacagca gcagcgtcgc ggttgccgtt
     7321 tctggcgcaa ataacgcacg cagtggtggc gcagcctgtt tttcgccagc gaacaccgcc
     7381 gactccggca gccagcgcat taatagcggc accagaatca acggcaccac accacctacc
     7441 caaaacaccg tttgccatgc taagtttgcc cccgcgaaac ccagtgtcgc cgccagcgcc
     7501 gcgccaatgg ggacaccgca atacatcagg ctcactgccg tcccacgaaa acgtggaccc
     7561 gcggcttcag acgtcagggc gatcagattc ggcaacgccg cccccagccc gacaccggtc
     7621 atcagccgcg caaagaccag tgaggggaaa tcccaggcaa tcgccgttgc cagtgagaac
     7681 aaaccaaaca gcgcaactga gccaatcaaa atgcgcttgc gaccataacg gtccgccagc
     7741 attccgccaa ccaacgcgcc gggtagcaaa ccgagtattc cggcgctgaa tatccagccc
     7801 atttgcattt tatcgagtgc gaaagcctgg gcgatgccac ccgccgcaat gccagccgcc
     7861 tgaagatcca gtccttccat cagagcgacc aaaaaacaaa gcccgatggt cagcatcagg
     7921 cgggttgaag atgatgaagg ggtacgagtc gacataagat gaacctcgtt gcgagtgcag
     7981 gtgaatcgcg gctgatacat tgtcagccgc gtaatcagaa ccatttaatg actgcctacg
     8041 ggcgaaatat aggccggata aggcgtttac gccgcatccg gcagtcgtgc tgggatgggc
     8101 atacaaaatg cacgttctcg gcagtcatca ttatttgttg ttgcgcagat ccagcgccac
     8161 gtcgacgatc atatcctcct ggccgccaac catccggcgt ttgcccagct caacgagaat
     8221 atccaccgcg cttaagccat aacgcgccgc cgccgtttca cagtgacgca ggaagctcga
     8281 gtaaacacca gcgtatccca gcgccagcgt ttcgcgatcg actcgtaccg gtcgatcctg
     8341 caacggacgc accaggtcgt cagcggcatc cattaacgca tagagatcgg tcccatgctg
     8401 ccaacccagt ttatccgcgg cggcaataaa aacttccagc ggcgcgttac ctgcgcccgc
     8461 gcccattccc gcgaggctgg cgtcgattcg gtcgcagccc tcttccaccg ccgcgataga
     8521 gttcgccacg ccaagactca ggttatggtg agcgtgtatg ccggtttgcg tttctggttt
     8581 cagcactgct ttcagagcgc ggaaacggtc acggatatcg ctcatgttca tcgcaccgcc
     8641 agaatccacc acataaatac aggtcgcacc gtagccttcc atcagctttg cctgctttgc
     8701 gagattctcc ggcgtggtca tatggctcat catcagaaaa ccaacggtgt ccattccgag
     8761 ctcgcgggca tactgaatat gctgggcgga aacatcagct tcggtacagt gcgtagccac
     8821 acgaaccacc cgcgcgccag cctgccaggc atttttcaga tcgtgaatag tgccgattcc
     8881 aggcagcaac aacgtcgcga ttttggcgtg cttcaccaca tccgccgccg cttcaatcca
     8941 ttcaaggtcg ctatgtgcgc cgaaaccata gttaaagctg gaaccttgca aaccgtcgcc
     9001 gtgggccact tcaatcgaat ccacgcgggc atcgtccagt gctttggcaa cctggcgaac
     9061 gttttccaat gaatactgat gacgaatggc gtgcataccg tcacgcaacg tgacgtccga
     9121 gatataaagt tttttaccgt tcatgccgct tctcctgcct tgcgcgccag tgactgggcc
     9181 attttttccg ctgtcgccag cgcactggaa gtcataatgt cgaggttgcc tgcataggca
     9241 ggcagataat gcgctgcgcc ttcgacttcc agccagaccg ccgttttcag tccggagaac
     9301 tgccccacgc ccggcaaatt aaccggttta tcctgcggga taacttcaaa ctgcacgcgc
     9361 tgtttcaggc gataacccgg tacgtaagcc tgcaccgcct cagccatttc attgattgag
     9421 gcttcgatat catcttgtga agcttcgtcg ctcaatacat acaccgtgtc acgcatcatc
     9481 aacggtggct ctgctgggtt aagcacaata atcgccttcc cttttgccgc gccacccacc
     9541 acttcaatgg cgcgcgaagt ggtttccgta aattcatcga tattggcacg cgtgccaggt
     9601 ccggcagatt tactggcaat agaagcgata atttcggcgt aatgaacacg cgccacgcgt
     9661 gaaactgccg ccaccattgg aatggtggcc tggccgccgc aggtgaccat gttgacgttc
     9721 agttgagcga cgttctcctc gaggttaacg accggcacgc agtaggggcc gatggcagca
     9781 ggcgtcaggt caattaagcg aatatccggt ttcgcttcgc gcaaagcggc atcgtttttc
     9841 acatgcgcac ccgcgctggt cgcatcaaat acaatgtcga tatcagcaaa ttcaggcatg
     9901 ttcatcaggc cgatcacccc ttcatgggtg gtggcgacgc ccatacgtct ggcacgcgcc
     9961 agaccgtcgg actgaggatc aatgccgacc atcaccgcca tctccagatg ctggccgtga
    10021 cgcaaaattt taatcatcag atcggtgcca atgttgcctg aaccgataat ggcgacttta
    10081 cgcttactca tgacagactt ccttttgggg ctgcgcttga aaatgtcgca gcaactgaac
    10141 ctatgccttc aatatgggct tcaaaacgat cgcccgcatt caccgccacc atcggaccta
    10201 atgccccggt aagaatgata tctccggcgc gcagcggttc acccaggctg gccattttgc
    10261 gtgccagcca gacggcggca ttaagcggat gtcccaggca ttcgctgccg cgcccgctgg
    10321 aaacctcttc gttattacgc gtcatcttca tggcgcagtt tttcaggtct aaccccgccg
    10381 gacgttgcgc cggaccgccg atgacataca ccccgcagga agcgttatct gccacggtat
    10441 cgacaaactg aatcgaccag tcgcgaatgc ggctccccac cacttccagc gccggaagta
    10501 cccattcaat ggcgttatac aattcgtcga aggtgatatc ggttgcgggt agatcgcggt
    10561 tcaacaccag tgcaatctcc gcttcaatgc ggggttgcag aacacgggaa aaaggaatgg
    10621 tttcgttatc gccataacac atgtcggcaa ataatgtccc gaaatccggt tgatcaacgc
    10681 ccagttgttg ctgcactttc ggatgcgtca gccccacttt gcgccccacc actcgccgtc
    10741 cctgagcgac gtcgtgttgc acattgatgt gctgaatggc gtaggccgct tcagcgttat
    10801 cgataccaat cagatcgcgc agcggcgcaa tcgcttcgcc ctgctctgcg gcgcgacgta
    10861 aatccgccgc cagttgctca agagtatgct tcgtcatgac cgttccttaa gggcgtgcga
    10921 ggaaattcag caccagttga ttgaaagcgt cggcatgttc ccactgcgcc cagtgaccac
    10981 agtcgcggaa gatatgcagt tcagaaccgg caatgccgga cagcagacgc agacccgcat
    11041 ccatcggcac aaagcggtcg ttgcgccccc agacaatcag ggtttgcgct ttgatttccg
    11101 ccagacgtgg gccaaaatcc gggaactgtt tcggattggc ttccaggctc ttaacgaagt
    11161 tttccaggtg atcgcggcgc gacagcatat tattcaggcg cgcttcaaac agggcgtcgg
    11221 tcaaatcgct ggtatcaaaa acgaagatat ccatcatcag cttcaggttt tcgatagtcg
    11281 gctgacgata aagctgattc agtcgcttaa taccttcggt tggcatcggc gtaaacaaac
    11341 tcatgccgcc cgtaccgccg cccatcagca ccagtttgcc gacgcgctcc ggccagttaa
    11401 gggtgaacgc cacagaactg tggccgccca tcgagttgcc cagcaggtgg attttggcga
    11461 tatccagttg atccaccacg cttttcagga ttcgcgcatt aagatccgat cgtgaaccac
    11521 tattaacgat cgaatcgctc ttaccccaac ccggacaatc cagcaggatc acccgatagc
    11581 ccgcctttac cagcggatcg atattgcggc tgaagttcgc ccagccagta gcacccgggc
    11641 cggaaccatg cagcaggaca acagtttcgt cgccttgtcc gcagtcatta aaatggatgc
    11701 gcagcgtttt acccgcttct tctacattca gaaaacggct ggtggcggct tcggtttgtg
    11761 gctgataact catcatcttc tcctgcatat tcagttctct gttctggcgc ttaacgagcc
    11821 aaatccggca atccactccg ggattgggcg gtaataacgc ccttcgctac gccagttgcc
    11881 aaacgcagaa atagcggcaa aagcggcgac ccaggttttg atttcatgtg tcgactttcc
    11941 ggcaatggcg gaaagctctt cgttactgac ggcatccagt tcctgtatgc gtccctgctc
    12001 cagcaaagtc atgaactggt tatcccaaat cgggttgagc ggatgcagcg ttctctgatc
    12061 ctcaacaaac ttctcagcgg cgctaatcac ccgttgctga cgcaattcgc gctcactggc
    12121 gggtaaatct ttcccgctcc ccaacagacg gtcgcgcata tgggcatcgg ctttcgccag
    12181 ttctggcacc ggcggctgat gggaaagccc accggaaccc aggaacagca cgcgtttatt
    12241 gagagtactg gtgaaacgtc caatggcttc ccccaacatg cgggtacgct ggaaaccggg
    12301 caacggcgtg gcgacaccgt tgatgaacac aggcagaacc ggcaccttat ccagcccacc
    12361 gagcaggaac tccagcggct gggcgaaccc gtggtccacc tgcatacagt aagaaacggc
    12421 aagatcgatt ccgctcttca tgacggcatg cgcacaggcc tccgccagct caacaggcac
    12481 gggcagctct cctgccgcac taccgaaatc accaattgcc gtcgctccaa cgcctaaaca
    12541 gaacggtggc atcacgtcat agaaaaagcc gttgtagtga tctggcgcaa acagcaccac
    12601 caactcaggg gagaaagccg caatacgctc gcgggcgctg gcaatcacgc cattgacctc
    12661 atcgagcacc tcttgcgccg ggtcgacata tcccaccagc ggcgagtggg aaagacagtg
    12721 aagataagcg tgcatatcag gctacctttt cgacagaaac gtcggcatca gggcgggtca
    12781 gcgtcattac cgacgccagt ttattcagcg tattacccag agtttgcgga atggcggtgg
    12841 cggcaacaaa gcgatccggg cgcatcacca ccagcgaagc attgtgttgc gcgaaccagc
    12901 tacgcaggcg accttgcgta tcgcccacgc gtagtacgcc gtcgtggtta tcctgtgcgg
    12961 tatgaatttg cacttccggc accacctgaa tgaagcgtgt gcccaacgcg cgccactgct
    13021 ggatttgctc gtcgctcatc ccccacagtg gattgcatcc ccagccaatt accgcgaagt
    13081 tcgcgccgat cgcgttatcg agcagcgtca cgtcgccgtt ttccagcgtg actttcggct
    13141 gaataaacat cttgccgacc ggagagtgct tcgcttcgcc ctcacgcacc agcgcaccgc
    13201 cgtaatattg cggcatcggc ttgaagcgca tttcgaggaa gtagcgtttt actggcggca
    13261 gataattcaa cagccaggaa acgccgtcac gtaacgtacc ctgccagcgt ttcggcggag
    13321 ccagcacgtt gcccgccgtc acggacaggt caatcatcgc tttggcgtga tcgcgacgtt
    13381 cttgttgata ggtatcgagc agcgcatcgc gggctttccc ctggataacc aacgccagtt
    13441 tccatgcgag gttaaaggcg tcgcgcatac cactgttata gccctgcccc tgccacaccg
    13501 gcatgatgtg cgcggcatcg cccgccagca gtacgcgatc aatacgaaaa cgttgcgcca
    13561 gtcgcgcgtt gtgggtgtag acacgctggc gaatcaattc aacattgtcc ggattaggca
    13621 gcactttgct taacagcttg cgcatatttt gcggctcacg cagctgttct tcggtttctc
    13681 ccggcatcac cataaattca aagcgacgta ccgcatgagg cagcgcggca gaaacatacg
    13741 ggcgcaccgg atcacaacac aaatagatat gcggcgtact taacggatcg ttggcgatat
    13801 ctaccacaat ccactgattt ggcgcagttt taccttcaaa cggcacattc agggtgcgac
    13861 ggacaaaact tgccccacca tcacaggcca ccagccattg ggctttgacc gtttcccgct
    13921 gcccttctgc cgttttcagg tgcaaggtca cttcgtcatc ttgctggctg aaggcctcca
    13981 gctcgcggga aaacaggcag cgcacattcg gaaaacgcga caatccttcc agcatcaccg
    14041 catcgacctg aggctgaata aaggcgttac ggcgcggcca gccaaattca tcggtcattg
    14101 gctgaatatc agcaaaacag cgaccttttg gggtgagaaa acgcatcgcg tgccacggcg
    14161 tagtgtgcgg cagaacatta tcgaccaggc cgaccgactg catggtgcgc agcgcctcgt
    14221 catcaatacc aatcgcacgc gggtagtcga tcaacttatc gagtttctcc accaccagca
    14281 cgtcaatgcc catctggccg agatagttcg ccatcatcag cccgaccgga ccggcaccag
    14341 cgatcgccac ctgaacgcta tggttaacag caggctggat gtcaggatgt tgtattgcca
    14401 tttcagtacc tcacgactcg gacaaaatgc cgttgcgcgc acagtacggc gcaacttatt
    14461 ttgttaaaaa catgtaaatg attttttatt gtgcgctcag tataggaagg gtgttttcgg
    14521 ctacaatcaa aacatgcccg aatgtgcacc aagtgcacaa cgttgtttta actatagaaa
    14581 tgtcaattaa tatgcagaac aatgagcaga cggcatacaa aaccgtgcgc ggcttaaccc
    14641 gcggtctaat gttattaaat atgttaaata aacttgatgg cggtgccagc gtcgggctgc
    14701 tggcggaact cagcggcctg catcgcacca ctgtgcggcg actgctggag acgctgcagg
    14761 aagagggata tgtccggcgt agtccctccg atgatagttt ccgtttgacc atcaaagtgc
    14821 ggcaattaag cgaaggattt cgtgacgaac agtggatttc tgcactggcg gccccactgc
    14881 tgggcgatct gttgcgcgaa gtggtatggc cgacagatgt gtccaccctg gatgttgatg
    14941 caatggtggt acgcgaaact acccatcgtt tcagccgctt atcttttcac cgggcaatgg
    15001 tcgggcgacg gttaccgctt ctgaaaaccg cctcgggcct gacctggctg gccttttgcc
    15061 cggaacaaga acgcaaggaa ttaatcgaga tgttagcctc ccgccccggt gatgactatc
    15121 aactggcacg ggaaccgtta aagctggaag ccattctggc gcgcgcgcgc aaagaggggt
    15181 acggacagaa ctaccgcggc tgggatcaag aagaaaagat cgcctctatt gccgtaccgc
    15241 tgcgcagtga acaacgggtg attggctgtc tgaatctggt gtatatggcg agcgcaatga
    15301 ccattgaaca ggcagcggaa aagcatcttc cggcgctaca acgggtggca aaacagatcg
    15361 aagaaggggt tgaatcgcag gctattctgg tggccggacg gcgaagcggc gtgcatttac
    15421 gttgacacca tcgaatggcg caaaaccttt cgtggtatgg catgatagcg cccggaagag
    15481 agtcaattca gggtggtgaa tgtgaaacca gtaacgctat acgatgtcgc agagtatgcc
    15541 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg cctgccacgt ttctgcgaaa
    15601 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
    15661 caacaactgg cgggcaagca gtcgttgctg attggcgttg ccacctccag tctggccctg
    15721 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
    15781 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
    15841 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
    15901 attgctgtgg aagctgcctg cgctaatgtt ccggctttat ttcttgatgt ctctgaccag
    15961 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
    16021 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcag gtccattaag ttctgtctcg
    16081 gcgcgtctgc gtctggcggg ctggcataaa tatctcacac gcaatcaaat tcagccgata
    16141 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
    16201 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
    16261 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
    16321 gacgataccg aagacagctc gtgttatatc ccgccgttaa ccaccatcaa acaggatttt
    16381 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
    16441 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct tccgcccaat
    16501 acgcaaaccg cttctcccca ggcgttggca gattctttaa tgcagctggc gcgacaaatt
    16561 tcccgactcg aaagcgggca gtgagcgcaa cgcaattaat gtgagtcagc tcactcatta
    16621 ggcaccccag gctttacact ttatgcgtcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    16681 ataacaattt cacacaggat acagctatga ctatgattac ggattcactg gccgtcgtat
    16741 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcggcacatc
    16801 cccctttcgc cagctggcgt aatagcaaag aggcccgcac cgatcgccct tcccaacagt
    16861 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
    16921 aaagctggct ggagtgcgat cttcctgacg ccgatactgt cgtcgtcccc tcaaactggc
    16981 agatgcacgg ttacgacgcg cccatctaca ccaacgtgac ctatcccatt acggtcaatc
    17041 cgccgtttgt tcccgcggag aatccgacag gttgttactc gctcacattt aatgttgatg
    17101 aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaat tcggcgtttc
    17161 atctgtggtg caacgggcgc tgggtcggtt acggccagga cagccgtttg ccgtctgaat
    17221 ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtactgcgct
    17281 ggagtgacgg cagttatctg gaagatcagg atatgtggcg aatgagcggc attttccgtg
    17341 acatctcgtt gctgcacaaa ccgaccacac aaatcagcga tttccatgtt accactcgct
    17401 ttaatgatga tttcagccgc gcggtactgg aggcagaagt tcaggtgtac ggcgagctgc
    17461 gcgatgagct gcgggtgacg gtttctttgt ggcagggtga aacgcaggtc gccagcggca
    17521 ccacgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacgc
    17581 tacgtctgaa cgtcgaaaac ccggcgctgt ggagcgccga aatcccgaat atctatcgtg
    17641 cggtggttga actgcacact gccgacggca cgctgattga agcagaagcc tgcgatgtcg
    17701 gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga
    17761 ttcgcggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga
    17821 cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc
    17881 attatccgaa ccatccgctg tggtacaccc tgtgcgaccg ctacggcctg tatgtggtgg
    17941 atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc
    18001 gctggttacc ggccatgagc gaacgcgtaa cgcgaatggt gctgcgcgat cgtaatcacc
    18061 cgagtgtgat catctggtcg ctggggaatg aatcgggcca cggcgctaat cacgacgcgc
    18121 tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag
    18181 ccgacacctc cgcaaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc
    18241 cctttccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctgcct ggagaactgc
    18301 gcccgctgat cctttgcgaa tatgcccacg cgatgggtaa cagtcttggc ggcttcgcta
    18361 aatactggca ggcatttcgt cagtaccccc gtttacaggg cggcttcgtc tgggactggg
    18421 tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcagct tacggcggtg
    18481 attttggcga tacgccaaac gatcgccagt tctgcatgaa cggtctggtc tttgccgacc
    18541 gcacgccgca tccggcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt
    18601 tatccgggcg aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc
    18661 tcctgcactg gtcggtggcg ctggatggca agccgctggc aagcggtgaa atgcctctgg
    18721 atgttgctcc acaagataaa cagttgattg aactgcctga actaccgcag ccggagagcg
    18781 ccggacaact ctggcttacg gtacacgtag tgcaaccgaa cgcgaccgca tggtcagaag
    18841 ccggacacat cagcgcctgg cagcaatggc gtctgacgga aaacctcagc gtgacactcc
    18901 cctccgcgcc ccacgccatc ccgcaactga ccaccagcga aacggatttt tgcatcgagc
    18961 tgggtaataa acgttggcaa tttaaccgcc aatcaggctt tctttcacag atgtggattg
    19021 gcgatgaaaa acaactgctg acgccgctgc gcgatcagtt cacccgcgca ccgctggata
    19081 acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggta gaacgctgga
    19141 aggcggcggg ccattaccag accgaagtgg cgttgttgca gtgcacggca gatacacttg
    19201 ccgacgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta
    19261 tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg
    19321 aagtagcgag cgatacaccg catccggcac ggattggcct gacctgccag ctggcgcagg
    19381 tcgcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta
    19441 ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct
    19501 tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt
    19561 ggcgcggcga cttccagttc aatatcagtc gctacagcca acaacaactg atggaaacca
    19621 gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata
    19681 tggggattgg tggcgacgac tcctggagcc cgtcagtgtc ggcggaattc cagctgagcg
    19741 ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg ggcaggccat
    19801 gtctgcccgt atttcgcgta aggaaatcca ttatgtacta tttaaaaaac acaaactttt
    19861 ggatgttcgg tttattcttt ttcttttact tttttatcat gggggcctac ttcccgtttt
    19921 tcccgatttg gctacatgac atcaaccata tcagcaaaag tgatacaggt attatttttg
    19981 ccgctatttc tctgttctcg ctattattcc aaccgctgtt tggtctgctt tctgacaaac
    20041 tcgggctgcg caaatacctg ctgtggatta ttaccggcat gttagtgatg tttgcgccgt
    20101 tctttatttt tatcttcggg ccactattac aatacaacat tttagtagga tcgattgttg
    20161 gtggtattta tcttggcttt tgttttaacg ccggtgcgcc agcagtagag gcatttatcg
    20221 agaaagtcag ccgtcgcagt aatttcgaat ttggtcgcgc gcggatgttt ggctgtgttg
    20281 gctgggcgct gtgtgcctcg attgtcggca tcatgttcac catcaataat cagtttgttt
    20341 tctggctggg ttctggctgt gcactcatcc tcgccgtttt gctctttttc gccaaaacgg
    20401 atgcgccctc ttccgccacg gttgccaatg cggtaggtgc caaccattcg gcatttagcc
    20461 ttaagctggc gctggaactg ttcagacagc caaaactgtg gtttttgtca ctgtatgtta
    20521 tcggcgtttc ctgcacctac gatgtttttg accaacagtt tgctaatttc tttacttcgt
    20581 tctttgccac aggtgaacag ggtacgcggg tatttggcta cgtaacgaca atgggcgaat
    20641 tacttaacgc ctcgattatg ttcttcgcgc cgctgatcat taatcgcatc ggtgggaaaa
    20701 acgccctgct gctggctggc actattatgt ctgtacgtat tattggctca tcgttcgcca
    20761 cctcagcgct ggaagtggtt attctgaaaa cgctgcatat gtttgaagta ccgttcctgc
    20821 tggtgggctg ctttaaatat attaccagcc agtttgaagt gcgtttttca gcgacgattt
    20881 atctggtctg tttctgcttc tttaagcaac tggcgatgat ttttatgtct gtactggcgg
    20941 gcaatatgta tgaaagcatc ggtttccagg gcgcttatct ggtgctgggt ctggtggcgt
    21001 tgggcttcac cttaatttcc gtgttcacgc ttagcggccc cggtccgctt tctctactgc
    21061 gtcgtcaggt gaatgaagtc gcttaagcaa ttaatgtcgg atgcggcgcg agcgccttat
    21121 ccgaccaaca tatcaggacg gaatgatcgc aatgaacatg ccaatgaccg aaagaataaa
    21181 agcaggcaag ctatttaccg atatgtgcga aggcttaccg gaaaaaagac ttcgtgggaa
    21241 aacgttaatg tatgagttta atcactcgca tccatcagaa gttgaaaaaa gagaaagcct
    21301 gattaaagaa atgtttgcca cggtagggga aaacgcctgg gtagaaccac ccgtccattt
    21361 ctcttacggt tccaacatcc atataggccg caatttttat gcaaatttca atttaaccat
    21421 tgtagacgac tacacggtaa cgatcggtga taacgtattg attgcaccca atgttactct
    21481 ttccgttacg ggacaccctg tacaccatga attgagaaaa aacggtgaga tgtactcttt
    21541 tccgataacg attggcaata acgtctggat cggaagtcat gtggtcatta atccaggcgt
    21601 caccatcggg gataattctg ttattggcgc gggtagtgtc gtcataaaag acattccacc
    21661 aaacgtcgtg gctgctggcg ttccttgccg ggttattcgc gaaataaacg accgggataa
    21721 gcactattat tacaaagatt ataaagttga atcgtcagtt taaattataa aaattgcctg
    21781 atgcgctccg cttatcaggc ctacaagttc agtgatctac gtaggccgca tccaacgtaa
    21841 acaaagcgca ggaacaagcg tcgcatcatg cctctttgac ccacagctgc ggaaaacgtg
    21901 ctggtgcaaa acgcagggtt atgatcatca acccaacgac gcacagcgca tgaaacgccc
    21961 agtccatcaa gtaattgcca ctgatactac gcagcacacc agaaaaccac ggggcaagcc
    22021 cggcgatgat aaaaccgatc ccctgcataa acgccaccag tttgccagca atagcgggtt
    22081 gcgcagagtg atcgagtgcc agcagcaaac agagcggaaa tgcgccgccc agacctaaac
    22141 cacacaccat cgcccagaag accggcaatt gcagcggcag ccagataaag ccgcagaaac
    22201 ccaccagttg taacgccagc gccagcatca aaagtttgcg ccgatcctga tggcgagcca
    22261 tagcaggcat cagcaatgcc cccgcagctt gcccaagcgt catcaacgcc agcaaggaac
    22321 cgctgtactg cgcgctggca ccaatctcaa tatagaaagc gggtaaccag gcaatcaggc
    22381 tggcgtaacc accgttaatc agaccgaagt aaacacccag cgtccacgcg cggggagtaa
    22441 ataccacgcg aaccggagtg gttgttgtct tgtgagaaga ggcgacctcg cgggcgtttt
    22501 gccaccacca ggcaaagagc gcaacaacgg caggcagcgc ccaccaggcg agtgtttgat
    22561 accaggtttc gctatgttga actaaccagg gcgtaatggc ggcaccaagt ccaccgccgc
    22621 ccattagagc cgcggaccac agccccatca ccagtggcgt acggtgctga aaccgccgct
    22681 taattaccga aggcatcact gcctgaatga tgccgatccc cactccacca agcagtgcgc
    22741 tgctaagcag cagcgcactt tgggggtaaa gctcacgcat caatgcaccg acggcaatca
    22801 gtaacaaact gatggcgacg ctgcgacgtt cgctgacatg ctgatgaagc cagcttccgg
    22861 ccagcgccag cccgcccatg gtaaccaccg gcagagcggt caacagggca gccacgctaa
    22921 agctcattcc gcttgcctgg cgcaattgcg gtagcagtgg cccgacggag gtgagcagtg
    22981 gtcgcatatt aagaccaatc agcaccagaa ccagcagcat cgtgaacgtc ttttgatggt
    23041 tacgcatggc tgtcagaacg gtttggtcgg cagatattta ccatctaagg tgatgaccgc
    23101 acgttcgcca ccctccgggt ccgccacttt cttaacgtcg agtttgaagt taatcgcgct
    23161 gataatgcca tcgccaaact tctcatgaac caacgctttc agggttgtgc cgtacacctg
    23221 caacatttca tagaaacgat acatcgttgg gtcggttgga atacggtcat caatgcagcc
    23281 acgcagtgga atcatctgca acagtagaat ggcgtcttcg tcgagatcca gcttcgctcc
    23341 gaccaggcgg gcggcgtcgg caggaagcgc ctgctgcccc agcaaagccg cggttacaaa
    23401 ggcttctgcc agaccggtgc cgtcggcaat ctcggcaaat gagagatctt ttttagcttt
    23461 gctgagcaaa atggcatcgg caagatcaag acgaatattg cggttaattt gcgactgaat
    23521 catggtggat ctcctgatgg tttaaaaata aggttacgct gcggtcggtt ggcgtagcgg
    23581 tatggcacaa acgcgaggat tagcggccag tggcacaaac tggcgggttg cgccgtcaaa
    23641 agctgcgatg ctgccgcttt caatgtcgta gacccagccg tgcagggcaa tccgcccctc
    23701 ttcgagcgcc aggcgcaccg atggatgagt ttgcaaatta gccaactgag caatgacgtt
    23761 ttcacgtacc atcgccgctg cttttgacgg taaatcggga tgcgggcgcg cctcattaac
    23821 gacgcgggct gaatcggcat aacgcagcca gtgggagacg gcaggcatat ggtccatgca
    23881 ctgacagctg gcaatggagg tcatcgcgcc acagttggaa tgaccgcaaa tcacaatgtc
    23941 agatacccga agtgcagcga cggcatactc caccgaagca gaaacgccac cgggttccgg
    24001 cccgtaggaa gggacgatat tgcccgcgtt gcgaataacg aacagatcgc caggctcacg
    24061 ttgcgtcacc agctcaggga ccagacggct gtcggagcag gagataaaaa gggtgcgcgg
    24121 gctttgctgt gtcgccagct gtttaaacaa ggcttcccgc ttcggaaatg cctcgcgctg
    24181 gaatttaagg aatccatcaa taatctcttt caccgttaac ctctgtctgt ctctggaatg
    24241 agaggcagac tactcgccga ttgtcataag gtaaaagact catttatgat gagttctatt
    24301 ggatttactt ataggttggc gaatgctctc tcgacatatc aattactttc ttgccgtggc
    24361 tgaacatggc agcttcaccc gtgcagccag tgcgttgcac gtctcccagc ctgcgctttc
    24421 ccagcagatt cgccagttag aggaaagttt gggcgtgccg ctgtttgacc gtagcgggcg
    24481 aacgattcgt ctcactgatg caggagaagt ctggcgacag tacgccagcc gggcgttaca
    24541 ggaactgggg gcgggtaaac gggcgattca tgatgttgcc gatctgacgc gaggatcgct
    24601 gcgtatcgcc gtcactccca cctttacaag ctactttatc ggccctttaa tggcggattt
    24661 ctatgcgcgc taccccggca tcacgctcca gctacaggaa atgtcgcagg agaaaatcga
    24721 agatctgctt tgccgcgacg agttggacgt tgggattgcc ttcgcgcctg tgcattcgcc
    24781 ggagctggag gcaattcctt tactgacaga aagtttagcg ttagtcgtgg cgcaacatca
    24841 tccgctggct gcctgtgaac aggtggcgtt gagtcgcttg catgatgaaa aactggtcct
    24901 gctcagcgcg gaatttgcca ccagagagca aattgaccac tactgcgaga aagcggggct
    24961 acatccacag gtggtcattg aggcgaactc aattagcgcg gttctggagc tgattcgccg
    25021 cacttccctt tccacattgt tgccagcagc gattgccaca caacatgacg ggcttaaagc
    25081 tatatccctt gccccgccac tactggagag aacggcggtt ttgttgcggc ggaaaaatag
    25141 ctggcagaca gccgccgcga aggcattttt gcacatggcg ttggataaat gcgcggttgt
    25201 tggcggaaat gaatcgcggt aaccactgac acaattgcct gatgcgctac gcttatcagg
    25261 cctacgagtt cagtgctcta cgtaggccgg ataaggcgtt cacgccgcat ccggcataaa
    25321 ctacgctcgc tttgtcaaca cacagcccag ccgatcaccg tttgtaatcg atggcttctg
    25381 gctgctccag atatacggtg gtttgtgctg gttgggtgct ggcaatcacc ttaccgccac
    25441 gtaccgaata acgtacagga acctgacggc gcagcgcgtc aaacccattt tcagccggca
    25501 ggataatcag gttggcgctg tttccggcgg caatgccgta atcctgcaaa ttcaacgtcc
    25561 tggcactgtg gtgggtgatt aaattcaggc catcgttaat ctgcccgtag cccatcagct
    25621 ggcaaacatg cagccccata tgcagcactt gcagcatatt cgccgttccc agcggatacc
    25681 acggatcgaa gacatcatcg tgaccaaagc agacgttaat gccggactcc agcatctctt
    25741 taacgcgcgt gatgccgcga cgttttggat aggtatcgaa acgtccttgc agatggatat
    25801 tgaccagcgg gttggcgaca aagttaatac cggacatttt cagcaagcgg aacaggcgtg
    25861 aggtatacgc cccgttatag gagtgcattg ccgtggtgtg gctggcggtg actcgcgcgc
    25921 ccattccttc acggtgcgcc agggcagcaa cggtttcgac aaagcgcgac tgctcgtcat
    25981 cgatctcatc acagtgaacg tcgatgagac ggtcgtattt ttgcgccagg gcgaaggttt
    26041 tatgcagcga ctccacgccg tattcacggg taaattcaaa atgcggaatc gcccccacta
    26101 cgtcagcccc taagcgtaac gcctcttcca gcaacgcttc accgttggga tacgacaaaa
    26161 tcccttcctg cgggaaggcg acgatttgca gatcaatcca cggcgcgact tcctgcttca
    26221 cttccagcat tgctttcagc gcagtcagcg ttgcatccga aacatcgaca tgggtacgca
    26281 catgctgaat gccgttggca atctgccatt tcagcgtttg ccaggcgcgt tgtttcacat
    26341 cgtcatgggt taataacgct ttgcgctcgg cccagcgttc aatgccttca aacagcgtgc
    26401 cggactgatt ccagttcggt tgtccggcgg tttgcgtggt gtccaggtga atatgcggct
    26461 ccacaaacgg cggtataact aaaccttgtt cggcatccag gctgttttca gttatgggca
    26521 tcaccccgga ttgcgcatca atggcgctga tttttccgtc cagcagatga atctgccaca
    26581 gcccctcttt gccgggtaac cgggcgttaa taattgtctg taaagcgtta ttcgacactg
    26641 ttagcctcca catgcgtcat tgcagccgtc gttttgcgat tcaaaatcgg gttaaggatc
    26701 agatagctca gcgcgccacc taataccgcg ttgaccggaa caatacccgg taaccagtgg
    26761 cctgcggcaa tccccaaggc gaccgccaga atcgccaccc aattgacact catcatacgc
    26821 gtggtcgcaa agtgctcata gcggcgacgg ttcatcagat agtcagcgat gatcacgccc
    26881 cctaccggag gaatagctgc cgaaaggaag gtcagccagc cgacaaaatt gttatacagc
    26941 cataatgcgc agaccgtacc gataataccg ttgattaccg aaagggtttt actcgacata
    27001 ccagtaatgt tggcgaaacc taaacccgac gcatagagcg cgttatcgtt ggtggtccag
    27061 atattcagcc ccagcaccac aatcgcaggc agcagcaggc cctgagcaat catcacatca
    27121 gagatatccg ccatccccag tgccgcagcg cccgctgcac cgaaaataaa catcaacgag
    27181 ttgccgagga aaaaggccac catcgccacc agcaccgcca gtttggcatt acgaccaaac
    27241 cggacaaagt cagcggtgag cgtgcccgca ctgataaatg accccacaac cagcgccagc
    27301 gcgacattga aatctaacgg ttgtgcggga acgaccgctt ttaatgcgtc caggccgccc
    27361 atgccattaa cggccagcca cacggaataa ccgccaaggc aggcgatagc cggaaccgca
    27421 atcaccgaaa gaaccgtcag cgccgaaatg ccaaagaaga cggtgacggt catcagtaaa
    27481 ccggaaacgg caatcagcaa attaatatcc agcccggttg ccttgcccac cggaatggca
    27541 aacatcgcca cgccaacgcc aaaccagcca acctgggtgc cgcccagcag cagtgaaggc
    27601 agccatgagc ctttaacacc aaaagagaag cgagcaagaa gatgagtggt caggccggtt
    27661 tttgcgccaa tgtaaccaag aaatgaagtg taaataccga ggagaagatt accgatgagg
    27721 actgcgagga agaaatcatg atagctaaga ccggttccga gagtgccgcc ggtccacata
    27781 ctggcggaaa agaaggttaa tcccagcatg acgaacgtca atgccaatac ccctttccgc
    27841 gccgactgcg ggactggccc ctggctaaag ttgttatctt gcgacacgaa attccccctt
    27901 tttgctgttt gaaacccaaa aaatccgccg cattctattc atctgaagat aaaaagcaat
    27961 cgttttcgta ggggaatata tttcttatat gaatgagtac aaaaaggcgg gggaaactta
    28021 tctgtgtaaa aacagtgggt aggcatgata agacgcgtca gcgtcgcatc aggcatctgc
    28081 gca
//
LOCUS       DAZGHF010000058        27820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGHF010000058 DAZGHF010000000
VERSION     DAZGHF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.058"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            98..361
                     /gene="yhcN"
                     /locus_tag="ACHK3A_002548"
     CDS             98..361
                     /gene="yhcN"
                     /locus_tag="ACHK3A_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO8740609.1"
                     /translation="MKIKTTVTALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(416..688)
                     /gene="yhcO"
                     /locus_tag="ACHK3A_002549"
     CDS             complement(416..688)
                     /gene="yhcO"
                     /locus_tag="ACHK3A_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO8740610.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(780..2747)
                     /gene="aaeB"
                     /locus_tag="ACHK3A_002550"
                     /pseudo
     CDS             complement(780..2747)
                     /gene="aaeB"
                     /locus_tag="ACHK3A_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
     gene            complement(2753..3685)
                     /gene="aaeA"
                     /locus_tag="ACHK3A_002551"
     CDS             complement(2753..3685)
                     /gene="aaeA"
                     /locus_tag="ACHK3A_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO8740611.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3693..3896)
                     /gene="aaeX"
                     /locus_tag="ACHK3A_002552"
     CDS             complement(3693..3896)
                     /gene="aaeX"
                     /locus_tag="ACHK3A_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO8740612.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4079..5008
                     /gene="aaeR"
                     /locus_tag="ACHK3A_002553"
     CDS             4079..5008
                     /gene="aaeR"
                     /locus_tag="ACHK3A_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO8740613.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5136..6581)
                     /gene="tldD"
                     /locus_tag="ACHK3A_002554"
     CDS             complement(5136..6581)
                     /gene="tldD"
                     /locus_tag="ACHK3A_002554"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO8740614.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(6737..10537)
                     /gene="yhdP"
                     /locus_tag="ACHK3A_002555"
     CDS             complement(6737..10537)
                     /gene="yhdP"
                     /locus_tag="ACHK3A_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO8740615.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGQVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTH
                     GDSTNSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10605..12074)
                     /gene="rng"
                     /locus_tag="ACHK3A_002556"
     CDS             complement(10605..12074)
                     /gene="rng"
                     /locus_tag="ACHK3A_002556"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO8740616.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(12064..12657)
                     /gene="yhdE"
                     /locus_tag="ACHK3A_002557"
     CDS             complement(12064..12657)
                     /gene="yhdE"
                     /locus_tag="ACHK3A_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO8740617.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12666..13154)
                     /gene="mreD"
                     /locus_tag="ACHK3A_002558"
     CDS             complement(12666..13154)
                     /gene="mreD"
                     /locus_tag="ACHK3A_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO8740618.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13154..14257)
                     /gene="mreC"
                     /locus_tag="ACHK3A_002559"
     CDS             complement(13154..14257)
                     /gene="mreC"
                     /locus_tag="ACHK3A_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO8740619.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(14323..15366)
                     /gene="mreB"
                     /locus_tag="ACHK3A_002560"
     CDS             complement(14323..15366)
                     /gene="mreB"
                     /locus_tag="ACHK3A_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO8740620.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15671..17611)
                     /gene="csrD"
                     /locus_tag="ACHK3A_002561"
     CDS             complement(15671..17611)
                     /gene="csrD"
                     /locus_tag="ACHK3A_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO8740621.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17763..18737
                     /gene="yhdH"
                     /locus_tag="ACHK3A_002562"
     CDS             17763..18737
                     /gene="yhdH"
                     /locus_tag="ACHK3A_002562"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO8740622.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEVVVTGASGGVG
                     STAVTLLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            18923..19310
                     /locus_tag="ACHK3A_002563"
                     /pseudo
     CDS             18923..19310
                     /locus_tag="ACHK3A_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            19715..20185
                     /gene="accB"
                     /locus_tag="ACHK3A_002564"
     CDS             19715..20185
                     /gene="accB"
                     /locus_tag="ACHK3A_002564"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO8740623.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20196..21545
                     /gene="accC"
                     /locus_tag="ACHK3A_002565"
     CDS             20196..21545
                     /gene="accC"
                     /locus_tag="ACHK3A_002565"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO8740624.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            21654..21896
                     /gene="yhdT"
                     /locus_tag="ACHK3A_002566"
     CDS             21654..21896
                     /gene="yhdT"
                     /locus_tag="ACHK3A_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO8740625.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            21886..23337
                     /gene="panF"
                     /locus_tag="ACHK3A_002567"
     CDS             21886..23337
                     /gene="panF"
                     /locus_tag="ACHK3A_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO8740626.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRTVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            23349..24230
                     /gene="prmA"
                     /locus_tag="ACHK3A_002568"
     CDS             23349..24230
                     /gene="prmA"
                     /locus_tag="ACHK3A_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO8740627.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            24559..25524
                     /gene="dusB"
                     /locus_tag="ACHK3A_002569"
     CDS             24559..25524
                     /gene="dusB"
                     /locus_tag="ACHK3A_002569"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO8740628.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            25550..25846
                     /gene="fis"
                     /locus_tag="ACHK3A_002570"
     CDS             25550..25846
                     /gene="fis"
                     /locus_tag="ACHK3A_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO8740629.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            25932..26816
                     /gene="yhdJ"
                     /locus_tag="ACHK3A_002571"
     CDS             25932..26816
                     /gene="yhdJ"
                     /locus_tag="ACHK3A_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO8740630.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAVLVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVANGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            26900..27079
                     /gene="yhdU"
                     /locus_tag="ACHK3A_002572"
     CDS             26900..27079
                     /gene="yhdU"
                     /locus_tag="ACHK3A_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO8740631.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(27082..27744)
                     /gene="acrS"
                     /locus_tag="ACHK3A_002573"
     CDS             complement(27082..27744)
                     /gene="acrS"
                     /locus_tag="ACHK3A_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO8740632.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT         6787 a         7386 c         6961 g         6686 t
ORIGIN      
        1 gtggtattaa ttagttcaat aattagtgta tacttgattt tgtgatatgg gtcacgaaac
       61 aaaggcccag ctaaaagatt atgtcgaggt aaaaatcatg aaaatcaaaa ccactgttac
      121 tgcattaagc gtactttctg ttctctcttt cggtgcattc gctgccgact ccattgatgc
      181 tgcacaagca caaaatcgtg aagcaatcgg gaccgtatcc gtaagtggtg tggcgtcttc
      241 gccaatggat atgcgtgaaa tgctgaacaa aaaagcggaa gagaaaggcg caacggccta
      301 ccagattact gaagctcgta gcggtgacac ctggcacgct acggctgaac tgtacaaata
      361 aaccctcatc gtcttgtccg acgatattgc ccccggttcg ggggcttttt ttgcgctaat
      421 gacgaacatt aaaacgcaaa tgcccttcca gctcttcctc tgcctcatca aacagcaata
      481 ttaacgcgcc aaaacggcga cgcgttttct ctcccagatg aacaaattca atctcaagtg
      541 gtagcggcag gacatcgttc attaacacat cccatagtga gtcgagatcg cgtaccttat
      601 ctttcgccag accaaaggct tggctgaagt cgcgataaaa atcctcctga ctctcaatct
      661 catcaaaatc aaaggtataa atattcatct gttgccaccg tcacgtttca cggcgagttc
      721 tgccgttcat cgctaagtat agccatgaaa aaaccgacgc ttcgggcgtc ggttttgact
      781 taactatcgg tcaacgcatg ttgatactta tggagcatcc ccgccagccg atgaaccggt
      841 tccgtcacct gcggtggcgc ttgccagatg cgtagctttt cctggtagat ttcaagttct
      901 tccagcaact ggccaaagta ccgacgacgt ttatcatcgc tacgggcaga tatcacatgg
      961 tctgctgtgc ggcgcatttg tcggtgaaac gccgataaat cctcgttaac cgggatcggc
     1021 gcatcacgca ggcgctggtg cgcgataatc atcgtcagcg ccaggcgaaa tttcggcaaa
     1081 tcccctggga acttattcat cagcaaaaac agctgctgat aaagtgccgg gaggtgattc
     1141 tctttacgac gtgccacatt ggtagtcatc gcggaaacag cggcagaaac aaactgatta
     1201 agcagtacac gtccagtcct gtcgcgcgat ttatcccgca ccagcaaaat aacggtgaac
     1261 gcgagcacac agccgacgat ttgccctaat gcgctgtcga gaaactgact gaaatggaaa
     1321 gtcatcgggt tatccagcac gataatattt atggtgctgg ccagcgcccc catcgagccc
     1381 agtcgccgtt tctgtacttc tataccgagg aaaaatccca gcactgccag gctaatacac
     1441 agcaataaca tgctttgttg ggtattaggg ataatcacca aaaagtagag cagccctaac
     1501 ggcagcgccg ccagcgtccc atagataaag tcgatcgcca ccatgcgtgg attcggcaaa
     1561 cgcattgcca gtgacgtcac gaccgcaatc atcaccattg caccactgcc ggaagtccag
     1621 cccgtccaca gccagaaaag cgtccccaga atgcaggaaa gtgtggttcg ccagaagtta
     1681 accatcgcat gatgacgttc ggctgactct actttgactt ccggttcgcc ttgcaggatc
     1741 tcttcttcag tagcgttgat ttttgtgtta ctgataacgc cgcgcttgag aagctgataa
     1801 cgcgttgccg ccgcgaccca gctataaatg gtgacaggcg tttcccgttc cccggtccag
     1861 gcgataactc tccgcaggcg tttgagctgc ttgtggacgt cctgcgcggt ttctaccggc
     1921 gtgtcaaaaa attcgcggaa agtatcagtg atcaattccg ggcgcgtatt ctgaataaga
     1981 taagtttcgc aggattgggt aatcagcgtc agcgatagcg tattgatcgc ttttaaacgt
     2041 cgattggccc gcgcccagcg ggaagattcc atattcaggt tgctgcgcat accttgtaac
     2101 gccgtggtgc gtcgcaccag atcgccccag gctttatcga caacttcacc atcgccatgt
     2161 ttgatacaga gttgcattaa ttgatattgc gcgaccagca aactttccag ctctcgatcc
     2221 acttcttgtt tgatcgatcg cggagaaaag agcaaatccg ccataatcgc acacacaata
     2281 ccgataacga tctcgctaca acgttcgacg gcaaactgcg gcgtaagcaa tggttccggc
     2341 taaatggtga tcacaatgat cagcgcggta taaccggcca gcccccacgc atacgagttt
     2401 tctattcgta ccagcgagga tatccaggta caaaaaccgg cccagataca gcacaccaga
     2461 atcatcaata atggtgcgcg gatcatcgca atgatgatta ccagtccagc aatacagcca
     2521 ataaatgtgc cgatgatgcg caaaaagcca cgatagcgaa tagcgccaga atacggttca
     2581 cctcccgcag caaaggccgg accggcggca acaatcgccg ctgtcagtac cgcccagcgt
     2641 ggcgtttcca gctggaagtg aaagccaaca aacagcgcca gtacaatggc ggtcgccagt
     2701 tttaccgcaa agcgaatatg ttgattagca atggagaaaa tacccatcgt gattaaccaa
     2761 actcacgcag gcgatgggcc attttacgga agaacgaatc ctggctttcg tcgcgatctt
     2821 gtttgccagt gaccaccact gtagcagtgg tgcccgcagg ccagatgttc tcttgctggt
     2881 tgtcgagacg aatacgtacc ggaacacgtt gcgccaaacg tacccattca aggttagagt
     2941 cgatggtcgc catccctttg tcgtcacgcg tgctgctggc gttggtgacc cctgcggcaa
     3001 cactatcaac agtccctttc agcactttgt tactgccaag cggcgtgatc tccgcacgat
     3061 acccagggcg caccccttcc agcttagttt cttccatata ggccagtaca tagaaggagt
     3121 tctgtttcac cagcgcaacc gccgttgatc ctcgagtaat aaactcaccg gtatagacgt
     3181 tgaggttggt cacccagcca tctgctggcg cgcggatgac tgtacgttca agatccagtt
     3241 ttgccagatc acgggtcgcc tgcgctttcg ctaactgatg cagaaccgtt tgtagtacgt
     3301 tgttggcctg atcgatctct tcgcgagaca tcgcctgcac accgagacgg ttacgacgtc
     3361 ctgcctcctg gcgtttctcc tgtgccagta cctgataata agcaacatcg gcttgcgctt
     3421 cctcaagcgc cttttgatag cgcggctggt cgatggtgaa cagtacctgt ccttttttca
     3481 ccagctggtt atcatgaaca ttcacctggg taatgagtcc agaaacgtcc ggcgcgatcg
     3541 caacgacgtc agcgctaaag cgcgcgtcac gcgtccacgg ggattcggtg taatagaccc
     3601 aggcattaaa aattgcgatg aaggccagaa tgactaatac gaccgtgatg gccgtacggg
     3661 agaattttct tattagtgtt ttcacttcaa cctcaaacga acagtcgcga tatcaaataa
     3721 aacaagcagc aatagagcgc ggtgttgaac aacgccggat gccagacaaa gtcgtagata
     3781 cctgttggca caagtacccg gcgcaccagc cagaaaatcg ccagtgataa aagcaattca
     3841 aaaaatatcg gtgggaagga cagcccaaac accacgataa cgggaaacag actcatgttg
     3901 accttggttg tatagagaga gcaggcgtta ttattttcag catctgtcgc cgcagagaag
     3961 ggcatggaaa gccgggcgag agcaacattg ctgtagattg atatttaata tattagcgta
     4021 actgttatgc tgttatctat attatgtgat ctaaatcact tttaagtcag agtgaataat
     4081 ggaacgacta aaacgcatgt cggtgtttgc caaagtagtt gaatttggct cttttaccgc
     4141 cgccgccaga cagctacaga tgagcgtttc gtccatcagt cagacggtgt caaaactgga
     4201 agatgagtta caggtaaagc tgttaaaccg tagtacacgc agcattggcc tgactgaagc
     4261 cggcagaatt tactaccagg gctgccgtcg gatgctccat gaagtgcagg atgttcatga
     4321 gcaactgtat gccttcaata acacccccat cgggacgctg cgcattggct gttcttcaac
     4381 tatggcacaa aatgttctcg ccgggctgac agcaaaaatg ctgaaagaat acccaggttt
     4441 gagcgtcaat ctggttaccg gaattcctgc ccccgacctg attgccgatg gtctggatgt
     4501 ggtgatccgc gtcggcgcgt tgcaggattc cagcctgttt tcccgccgtc tgggcgcgat
     4561 gccgatggtg gtgtgcgccg cgaaaagcta tctcacacaa tacggcatac cggaaaaacc
     4621 cgccgatttg agtagtcatt catggcttga atacagcgtg cggcccgaca atgaatttga
     4681 actgatcgca ccggaaggga tctcgactcg cctgatccca caaggaagat ttgtgactaa
     4741 tgatccgatg acgctggtgc gctggctgac ggcgggtgcc gggatcgcct acgtgccgct
     4801 gatgtgggtg atcaacgaga tcaatcgtgg ggaactggag atcctgctgc cgcgttatca
     4861 gtcagatcca cgcccggttt atgcgttata taccgaaaaa gataagctgc cgctgaaggt
     4921 acaggtcgtg atcaactcgc tgacggatta ttttgttgag gtcggtaaat tgtttcagga
     4981 gatgcacggg cgcgggaaag agaagtaatt catattgtac tgttacgttg tacaaacctg
     5041 tgccaacggg ttcccctcac cctaacccca gaggagcgag gggaccgttc gtgcacgtag
     5101 aaagattaat tatccttctg aaaatactga aattattacg cagtaccgcc aacagtcagg
     5161 ttatcgactt tcaacgttgg ctggcccacg ccaaccggca aactttgccc ttctttaccg
     5221 cagacaccca cgccgttatc cagtttcagg tcgttaccca ccatcgaaat ctgctgcatg
     5281 gtttcgatac cggaaccaat caacgttgcg cctttcaccg gcttcgttac tttaccgttt
     5341 tcaatcagat atgcttctga agtggagaaa acgaatttgc cggaggtgat atccacctga
     5401 ccgccaccaa agttcggtgc atagataccg tactcaacgg attcaataat ttcctgcggg
     5461 gtcgatttac ccggcagcat ataggtgttg gtcatacgcg gcatgggcag atgggcgtag
     5521 gattcacggc gaccgttgcc agtcggcgtc atccccatca aacgcgcgtt gagtttatcc
     5581 tgcatgtagc ctttcaggat gccattctca atcagcacgt tgtactggcc tggcgtacct
     5641 tcgtcatcaa tcgccaccga accacggcga tcgaccatcg taccgtcatc aaccacggta
     5701 cacagttctg aagccaccag ctccccaacc tgtccactaa atactgaagt gccacggcgg
     5761 ttgaagtcgc cttccagacc gtgacctacc gcttcatgca acagcacgcc cggccaacct
     5821 gcgccaagta ctaccggcat ggtgcccgct ggtgcagcaa cggcagaaag attgaccagc
     5881 gccatacgca ctgcttcttt tgcccatgca tccgcacgga cttcgccgtc gagatcggca
     5941 aggaagaact cataaccaaa acgaccgccg ccgccactgg caccgcgttc gcgtttgcca
     6001 tcttcttcga ccagaacgct cacggaaaga cgcaccagcg gacggacatc cgccgctagc
     6061 gtgccgtcgg tggccgcaac cagaattaat tcatagacac cactgaggct ggcagtcact
     6121 tcctgtacgc gcttgtccgc ttcgcgggca accttatcga cgcgacgcag gatatccagc
     6181 ttctcttcac ggctcatgct ttgcagcgga tctaccgagg tatacaacgg gctatgctct
     6241 accgcgccca gcgtctgtac tttaccatca ccactatcac ggacgatggt gcgcgccgct
     6301 tgtgcactct gttccagcgc cagcaggctg atttggtcag cataagcaaa tccggttttt
     6361 tcaccactga ttgcacgcac gccaacgccc tgatcgatgt tgtaagaacc atctttaata
     6421 atacggtctt ctaaaaccca ggattcgtga tagctcgact gaaaatagag atcgccataa
     6481 tcaaggcgac gttcggccag ttgaccgagg atcgcgaaca agtcctgatg tttcaggccg
     6541 ttcgccgcta gcaattgttc acttaccagg ttcagactca tcgtttttgc tactcgttag
     6601 ttactgcagt agaggatttt ttacggctgc cggatgcggc gtgaacgccc tgtccggcct
     6661 acggttatgt tctggctcgt tggcctttgg caacgattat cctatgagat tggggcaatt
     6721 acgcgccctc gtcaaatcat tgcgcttttt ctttacgcgg ttgtcgcaac acttcgttga
     6781 tttgcggatc gtccagcgga cccgaaatgt gatagcgcaa aatggagact ttgctccaca
     6841 gcggtcccag cactttactg gcggcaaaca ctgccgcgcc aacaatgggg ttaaccgcaa
     6901 aggccgcagc cacacccacc gtcgcagaaa tctctggtgc gacaaccgct tccatattca
     6961 ggtcgcgacg taccagattt accgaccctt tcatagcgat gtccgcttcc aggccatcca
     7021 ccagcgtgtc gtcggtgtgc ataacgccgt ctttaatcca cgcggtgctg cgaatggagt
     7081 caaaatagaa cccttcgcca aaagtgtctc tgaaatcaaa acgcagctta cgcatcaggg
     7141 catctacgct caataagcgc agcaattgcc cggcatgtcc ggtattgatt tcggtaattt
     7201 cgcctttacc cagttgagta tgaatgatgc cattcaacgt cgcctcatct ggttgccagg
     7261 gtgctttgcg ccagtgtaaa tcgtaatcca cattaaatga cgactggcgt atgggcgtcg
     7321 tgacaccaaa aaattctgcg gcggcatcaa ttttctgccc acgcagtttt cctttcagcg
     7381 aggtgcgttc attccccgga ttgttaaccc attcaccatc ggcagtgagc cgcgaaaaac
     7441 cagtatcaat cagtccatta gtcagcgtta atgtattgcc agaaatggtg atatcactgt
     7501 caatgcgccc gaatttttgc ccccagaacc agcactctgc gcatcgtatt tgtgcgtccg
     7561 gccagccacg gaagctaatg cgctccgttg tcgggaaagg tgatgaattc gttgagtcac
     7621 catgagtttt cgccacgctt gggttgtaat aaagatattt gatattcgcc agccacggcg
     7681 cgttattacg catcgccagc gtggcgttga tttcacgccc ttgcgcctca accagggtgc
     7741 catttgccgt cggttgcgaa acaatgctca ggttattcca ttgctgattt cccagcgaca
     7801 acataggcgt acgtaacgtt atgtgttgtg ggaaactcgc tgcaccaccg acactctccg
     7861 cagcgccttt ctggaacagg gccagccact cggcaccatt catcggcggc atattgagtt
     7921 caacgccact ttgttccggc aacggcggga gcgttttact gtctgccgcc caaatagcac
     7981 ggtcgagcgt cagcttttga ccgagcaacc agcggctatt gaaatggtta tctgcaccag
     8041 cctgtccggt taactcaaag ctgttgagat tgccatcaac cttaacgttt accggcagtg
     8101 gttcacccgc aggtttggct aacggtgaag gtaagtgact gctcacattc ttcaggtcgc
     8161 cgttcagctc tacgttatag gtcgcgccag catgataagg cagatcaatg cccactttac
     8221 catcccacgc cacgctgcca cttaatgctt cgttcaccgc ttcaggcaga acgccggttt
     8281 tcgctggttg ccagttaccg ttgagattca ccgcgacctg gtaggctttt gccccttctt
     8341 tggtggaaaa atccacgttc aacggctgat taaaccagct tgctgtcagt ggttcacttt
     8401 gcagatcgcc attgataaag ctgaatttac cgctcaaatt tttcagggtg ctgtcgagtg
     8461 gtttgataaa cagactgtta ttacgcagcg tcacatcacc tttcgcggtt accagttcgc
     8521 cgttcagcgg gatatcaaga tgtaagcgag cattcacatc gccgtcgagc tggagttctt
     8581 gcagggtcgc acccagagaa tctttcagcg gtgtctcatc aaagtaaggg ccaacggctt
     8641 tacccggacc tttaatgtca gcgtcaatca gcagtttttc ttttgagtag tcagggatca
     8701 ctgcggtgag attacttgcg cgcacgccgc ccagattaac gccatcggtt ttcatccata
     8761 aaccgtcgtt aataaagtcc agttcaatat caaggttagt taatgcaggc cagtccggct
     8821 ggaaggcaaa cttcgcgttg cgcagcggta ccagcacttc aaactgacct tcgttgtgtt
     8881 tataggggaa gagttgcgga ttgccaccat aaaccagcgt cgcgttatcc gcttcaccgc
     8941 cctgaatcgc gccacttaag taatcaacca ggtctttacc catcaagttt tccgggaaat
     9001 agcgccaggc ttgtgaacca tcatcggtac tgatgccagc cagaataccc agccagggtt
     9061 catcgttagc tggttgcagg taacgaaaac cgccgcgcgc atggacggct ttggctttaa
     9121 cgtcaatatt acgcccatcc agctggaaac ctttgtcatt gttcagccag cttatcgttg
     9181 cctggccgtc ggcgatttcc agtggcgcac ggaataccgt ttcgtaaggc atctttgcct
     9241 gcttcatcga cgcagtaagc aaaccatttt ctacgctgcc ggaaagcgtc ccggagaagt
     9301 gttccgcacc cggtaataat ttccattgct tccaggccag atcgctccac gatgcctgaa
     9361 aacgggtctt gtctgccgcc tgaagcggga tatccagcgc cagcgtgtta atcttgccgc
     9421 tcggttgtgt ggagcgccaa acatcaccca gtgcaggtga aagtttcgcg gccagcgggc
     9481 gtatgccctc caggcctgcc agctccagat tactggcgcg aatccggagt tcgtcactgc
     9541 gtttattgtc tttgccgcca acgtcctgtt ccggtatcca ggccagcgtc aatgctccgc
     9601 tcggccaggg tttgccgtcc atcgtgatcc gtgtatcggg aatagagaac tgccagcctg
     9661 gattttcacg cgtaatatgc gcggtcagat tatccaccga cagcgtatgc gtttgcttct
     9721 cgcccaacca gctggcaccg ccctgtttca gccagacgtc accgccggtt acatcgcctt
     9781 tgtcgatcgt catccagcct tcaagggaga actgtgccgt ttccagcgca atattgtcct
     9841 gcatccattt accgagccac ggcttcaggt cgatgtcatc cgcctggagc cagacgcgac
     9901 cattgcttaa caacccctca tcatcgcgca aatccatgcg cacctgcatc acgccgtgct
     9961 gtccggtaag gctggagagg cttacctggc cttctgcacg gtgtcgacgt ggatcgttca
    10021 gccaggtgag ctgtgggatc gccagctcgg cgcgctgacc ggatggcgtc aggaaactga
    10081 cttcactgtc gcggagatcg aaatggtcga actgacgaag aaacagatcg ctgatgtgac
    10141 tggcttccag actatcatta ccaccgccgc tggtgatagg agtgttggtg cgaaagcgca
    10201 gctgccagaa agtgaggtcg cgaaactgcc agcgcatatg taacaggctc tgccagacat
    10261 ccagcgccag agtaacgcgt ttaaccgaaa attcgccgcc atcttttagt tctgcacgga
    10321 tgtcgtgcgc ttcaagcgtc ggaccaaaat tctgccagct ggctgagagc tgactggctt
    10381 ctaccggcat gccagtcgcg gattctattt tgttgaggat ttccggacgc caggcgtcaa
    10441 gatgcggtaa agcaatacgc aggccgctaa ccagcagggc agcgatcaca acgagcgcgg
    10501 ctccagtaag cagtaaaatc cccggcaatc gcctcacccg tcactccttg tctgctaaaa
    10561 atgtgactca aaaacccttt gccggatggc ggcccagcat ctgtttacat cattacgacg
    10621 tcaaactgct cctggttata gagcggttca atttgtactt taacctgttt accaacgaaa
    10681 atttccactt ccgccagcga gtgtgactct tcgcctttca aggcttcagc tactgccgga
    10741 gaagcataga ccaggaaacg gtcggaatca taagcatggt gtacacgaac aatctcgcgc
    10801 atgatttcat agcataccgt ttccacggtt ttcaccgttc cgcgaccgtg gcaggttggg
    10861 cattcgttac atagtacgtg ctcaatgctt tcgcgggtgc gtttacgcgt catctccacc
    10921 agccccagcg ccgaaaaacc attaacgctg gttttcaccc ggtctttgct caacgcctgc
    10981 tccagcgagt gcagcacgcg gcggcggtga tcttcattat tcatatcgat gaaatcaata
    11041 atgataatcc cgcccagatt acgcaaccgt aactggcgag cgatagcctg cgtcgcttca
    11101 atattggtat tgaaaatggt gtcgtccaga ttgcgatgac cgacaaacgc cccggtattg
    11161 atgtctacgg tggtcatcgc ttcggtctgg tcgataatca gatagccacc ggatttcagt
    11221 tctactttgc gttccagcgc tcgctgaatt tcgttttcga catcaaagag atcgaaaatc
    11281 ggctggcgtc cggtgtaatg ctccagcttg ctggtcatct cgggaatgta ctccgaggta
    11341 aactccagca atgcttcgta agtcaggcgt gagtcaacgc gaatgcggtc gagttcggcg
    11401 tcggcgaaat cacgcagaac acgctgcgcc agcgccagtt cgccgtacag ctgataacgg
    11461 gtctgtgggc gctttttacg ctccataact ttggtccaga cgcgtttcag ataagcggca
    11521 tcggaggcca gttcagcctc gccaacccct tcagctgcgg tacggatgat aaacccgccc
    11581 tgctcgtcgc aatactctgc gaccactttt ttcaggcgtt cacgttctga ttcgctttca
    11641 attcgttggg aaaccccaac gtgagaagcc cctggcataa ataccagata gcgagaaggc
    11701 agcgtgatat cggtggtcag gcgcgcaccc ttagtgccaa gtggatcttt caccacctgc
    11761 accatcagat cttgcccctg acgaaccagt tccgagatgt cgcgcaccgt gaattgcttt
    11821 tgttcttcac ccgccacaca ttcggtgtgc ggcatgatgt cagatgcatg aagaaacgcg
    11881 gctttatcca gcccaatatc tacaaaagcc gcctgcatac ccggaagtac acgacttaca
    11941 cgacccttgt agatattgcc tactattccg cgtcgcgcct cacgttcaat atgaatttcc
    12001 tgcagaatac cgccatcaat atacgccact cgcgtttccg aaggcgttac gtttactaac
    12061 aattcagccg tcatgtttat cccttttctc acgcagtgcg ttaaaattac ttaataattc
    12121 atacgtttca accagcggta agccgactac ggcgtgatag ctgccattta tcttcctgac
    12181 aaaacagcca cccagcccct gaataccgta tgcacctgct ttatctaacg gttcaccgct
    12241 ggcgacatag cccgcgatgt cttcgtctgt taacgttctg aaagtcacat cggtgaccac
    12301 caggcaatcg agaatgtgct ggctgtcggc caacgccact gccgtcatta cctgatgggt
    12361 ctgacccgat aatttgcgca acatctgcgc cgcatgctct gcgtcgcgcg gtttctccag
    12421 tacttctccg ttcaggataa cgatagtatc cgcacccagc accgggagat cctgcgccgt
    12481 ttgcgcgaca cccgcctgtg ctttctcgcg cgccagacgc acaacatact gctgcgcgct
    12541 ctcctgcggc tgacgctgct cctcaatgcc cgtaacaata cgttcaaagg ttacgccaag
    12601 ttgcgcaagt aactcctgac gacgcggaga accggaagct aaatacagag aagtcataga
    12661 aacctttatt gcactgcaaa ctgctgacgg actttacgca tcagcaagaa aatccacggc
    12721 cagagcaccc cattgactac gctactccag aacacttccg gtctgaaaga gacgttaatc
    12781 actaaaaact ctgcccagaa aacaataata tccaccacca gcgaaagcaa catgacgacc
    12841 agcgcctgct gccataatgc gaggttgcgg aaaagctggt atttcagcgc caccaggtaa
    12901 gcaatgatgc tcatcgccaa tacgcgtacg ccaagcgtcg agccgctgat cagatccagt
    12961 atggcaccca tcacaaaacc tgtgcccaca tttacgcgat gaggcaaggc caggatccaa
    13021 tacaacaaga tgagtaacac ccagtttggc cggaaaacaa tcaggttatc cggccagggc
    13081 atgatttgca gcaacagcgc aatgaggaaa gagagccaga ttacccagcg tccctggcta
    13141 cgatagctcg ccactattgc cctcccggcg cacgcgcagg cggttgagca ccactttgcg
    13201 gcggcgtagc cctttgtgga gagcgattag cagcgggctg tgtgggcgca gcaggcgcag
    13261 taactgcatt tcctgtcgcc ggttgctgcg gagtcggctg agtgatcccc gttgccggtt
    13321 caggtaactt tggccccatc gcgtctggcg aaggcaatac ctgcggcatc atctgcatca
    13381 aacgttcatt agcaacacga tgcacctctt ccggcgtcat cgggttagcg ccgttacgat
    13441 ccgcgcccca cagcagcagc agataacgca aacgttgcag tcctgcagtc ggacgcgcct
    13501 gaatcacagt ataagcgcgc tgggtatcga gttttacgga agagacaacc gcgaccggat
    13561 agccttccgg gaaacgaccg cccagaccgg aagtcaccag cacatcacca acacgaatgt
    13621 ccgtattcgc tggcagatgc tcaagctgca aatcatccgt acaaccgtta ccggctgcaa
    13681 ttacgcggat atcgttgcgc agcacctgga ttggcagcgc gtgggtcgca tcacaaatca
    13741 gcagcacgcg gctggtcagt ttagcgacgg ccaccacctg accaacaaca cctttgtcgc
    13801 tgataaccgg ctggccttca taaacgccat taacgctacc tttatcgata acaacttgat
    13861 cgctataagg atcgttaacc gtggagataa cctgagtcac cattttctgc tcatcctgac
    13921 gcagcgggga acccagtagc tcgcgcagac gcgcgttctc ctgtttgtat tgtccaagca
    13981 tcagcagttc actgtttttc agcaacagtt cctgacgtaa cgcccggttt tcaagttcta
    14041 gttggtcacg cgaggccagc gtctgcgata cgccatccag caattcacga ggagcattgg
    14101 aaacaaagta gaaaggactg acggcggtat ccatataagt acggatttga ctgaacgtcc
    14161 ccaggcggct gtcggcaata ataatgccga gcgccaccag caccgccaga ataaggcgaa
    14221 tctgtagcga cgggccacgg ctaaaaattg gcttcataag ttatgcgtat tctcgtatca
    14281 gaccaggcag ggtaaacaga cacttcccct gcctgcatcc gattactctt cgctgaacag
    14341 gtcgccgccg tgcatgtcga tcatttccag cgctttgccg ccaccgcgcg ccacacaggt
    14401 cagcgggtct tcagcaacaa cgactggaat gccggtttct tccattaaca aacggtcaag
    14461 gttacgcagc agtgcgccac caccggtaag caccatgcca cgctcggaga tgtcggaagc
    14521 cagttccggc gggcactgtt ccagtgcaac cattaccgcg ctcacaatac cggtcagcgg
    14581 ttcctgcagt gcttcgagga tttcgttgga gttcagggta aagccgcgtg gaacaccttc
    14641 tgcaaggtta cggccacgaa cttcgatttc acgtacttca tcgcccgggt aagccgaacc
    14701 gatttcgtgc ttgatacgtt ctgcggtggc ttcaccgatc agagaaccgt aattacgacg
    14761 tacatagttg atgatagctt cgtcgaaacg gtcaccacca atgcgcacgg aagaggagta
    14821 aaccacgccg ttcaaggaga taacagcaac ttcagtagta ccaccaccga tatcaaccac
    14881 catagaaccg gtcgcttcag aaaccggcag gccagcacca attgcggcag ccatcggttc
    14941 ttcaatcagg aagacttcac gggcaccagc gccctgcgcg gattcacgaa ttgcgcggcg
    15001 ttcaacctgg gtcgcgccaa ccggcacaca aaccagaacg cgcgggcttg gacgcataaa
    15061 gctgttgctg tgcacttgtt tgatgaagtg ctggagcatt ttttcagtca cgaagaagtc
    15121 ggcgataacg ccgtctttca ttgggcgaat agcagcaata ttgcccggcg tacggcccag
    15181 catctgcttc gcgtcatgac ctactgcagc tacgcttttc ggtgaaccgg cacgatcctg
    15241 acgaatggcc accacggaag gctcattcaa tacgatgcct tgtcctttta cataaatgag
    15301 ggtattcgca gtacccaggt caatggacaa gtcattggaa aacatgccac gaaatttttt
    15361 caacatacta agggataatc ctgaaagctg gggcggaaaa gaaaatccgc ttactttacc
    15421 aaccacacgc agcagcgaca aggcgcaaaa atcatctgct acggtgaaaa ttagtgcagt
    15481 tcgtttcctt tgttacaaat ctctgcctga gtccagaaag gcttaatgca tcagcagcat
    15541 tcctcgcctg tttgcaaccg cgtaaggtca ttcatctgca tatgtgctgc aacaatctgg
    15601 cgagcagaca agcacactcc catgagacgc agcgcgcgtt attctacgtg aaaacggatt
    15661 aaacggcagg ttaaaccgag tatctttgtg aatatttttt cacgttagta tcaagtggct
    15721 gtgaggacgc gaaaaaatcc ccttgcccac ctgtaacacc gcgctgaatc agggtctgcc
    15781 actcgcttcg cgaacgcacg ccggtggcgt aaacctgggt gctggtcccg gagcaggctt
    15841 ccaccaggct ttgaaccagc agctgattct ccgttcgctt ctcaatgttt ctgaccagcc
    15901 ccggatggag cttgagtaac tcaacattaa gttctttgat ccaactggtg cttaccagcg
    15961 tcaaaccagc ctggttgacg gctacccgta cccctaaagc attcactaaa cgaataacag
    16021 gttgtaagcg actgatatgt tgaccaacat cggcctctgc aagttcaata attatgcgtc
    16081 ttcgttgcga tttttcacat tgcattaacg tatcgcgcag ccaacgctga aaacgcgggc
    16141 gaatcagcga ctcaacggta acctgaatcg ccagattttc ctctggccag taacgcaata
    16201 gtggaataag acggctgatt agcaggcggt catactcttc cgataaacca aactgcaaga
    16261 ccatcggcat atactctgcc gagctaacct cttcattacc atcgaagatg cggcacatga
    16321 gttcacgatg atgaacccgg ccttcgcgag taaccgccgg tttttgataa aggcgcgggc
    16381 cgccgcgact gagcatttgc tcgataagcg tacgccagcg aacattaccg cgtccttttt
    16441 caggcaacga gtcatcgtaa atagcccagc tattgccgcc ctgcaatccc gcattacgcg
    16501 tggcagactc tgcatgttcc attacctgct cggtatcctg accactacgc caggcacaga
    16561 taccaatgtg gatcatatcg tcgcgatcga gcattttatt gttcggcaag gtatcgacgg
    16621 ctttgattaa ctgactggcg atgctctctg cctcttttaa cgtccggtgc ggtaacagcg
    16681 cagcaaaatc actgcggtgg taacgcgcca gcagtgcgcc agggtagcgc atcataaatg
    16741 tcgatagcag attcgtcaga gagaagaact gttcttcaac ctggctgtgc ccccaggtat
    16801 cactcaacat attgaaatcc ggcagacgaa tcatcatcac gatcccgtgg gtacctactt
    16861 tctcttgatc ttccagtaac gttgctaact gattatcgaa aaagagtcgg ttattgaggc
    16921 cggttttcat gtcctgggcg gcataagagc ggatcagcgt atcaagacgg ctgtgttgtt
    16981 cgcgtgcgtt ctgaatttca caaagcagcg tatccagcgc actgctggtt ctgggcggcc
    17041 attcatagat ggttcccaac acattagagc cacgctcacc gtttaagata cgagtagccc
    17101 gggtttccag caattcttgc ccggcaagtt gccgttgtaa ccagcgtacc gccaggaaga
    17161 gcataagaat gataaagcca atcgcccccg tgagcggcgc ggtagtcatt aaagaatgga
    17221 aatagttgcc catcggatcc tgataaacca gacgcagcga catccccgga tgctttatca
    17281 acggaacgct cagttcacga aacagatcgt tggtgccaac cggacgataa ctaccatttc
    17341 tggccagggt ataaacctgc ttatcaccat ggagcaggtc tacacgaacg atatctgccg
    17401 acatcattaa ttcggtaatt tgtggcctta atgtgctgaa gtcattcgac actaggtggg
    17461 tatcaatcgc cgtcgccacc gcctgaacac gatgactaaa cttatactga atggcgttgt
    17521 agaaacttag cgaacagccc agcaaagtca caaaaattgt taacccggtg agcagcgtga
    17581 caaaggccga aaatttcgtc gttaatctca tccttgtgtt aactccgata gtgaggaagc
    17641 gggcatacta gcaaatcaga tttatctcgc aatttattgc gcttcatcgg ctttgctttt
    17701 ccattagcga gtatagtctt cagaaattat tttccaatcc atcatgcaca tgaggaccac
    17761 ttatgcaggc gttactttta gaacagcagg acggcaaaac tctcgcatca gtacagactc
    17821 tggacgaaag tcgcctgccg gaaggcgatg tcacggttga tgttcactgg tcgagcctga
    17881 actacaaaga tgcgctggcg attaccggta agggaaaaat catccgaaat tttccgatga
    17941 ttcctgggat tgattttgcc ggaactgtac gcaccagcga agatccgcgt tttcatgccg
    18001 gtcaggaggt gttactcact ggctggggcg ttggtgaaaa ccactggggt gggctggcgg
    18061 aacaggcgcg agtgaaaggt gactggctgg ttgccatgcc gcaagggctg gacgcgcgta
    18121 aagcaatgat tatcggtact gccggtttta ccgccatgct gtgtgtgatg gcgctggaag
    18181 atgccggtgt tcgcccgcag gatggggagg ttgtcgtgac gggtgccagt ggtggcgtcg
    18241 gcagtaccgc cgtcacgctg ctgcataagt tgggttatca ggtcgttgcc gtttccggtc
    18301 gcgaaagtac ccatgaatat ctgaaaagtt taggtgccag ccgtattctt cctcgcgatg
    18361 agtttgctga atcccgtcct ctggaaaaac aagtctgggc tggggcaatt gacaccgttg
    18421 gcgacaaagt gctggcaaaa gtgctggcgc aaatgaatta cggcggctgc gtggcggcct
    18481 gtggtctggc gggtggtttt actctgccaa ccacggtcat gccatttatt ctgcgtaatg
    18541 tccgtttgca aggggtggat tcagtaatga cgccaccagc acgccgcgca caagcctggc
    18601 agcgactggt cgccgattta ccggaatcat tctataccca agcggcaaaa gagatatctc
    18661 tggcagaggc accgaagttt gccgaggcca tcattaataa ccagatccag ggtcgcacgc
    18721 tggtgaaggt taactaacca tttagcaggg aataataaga gagggaactc attttgaaat
    18781 cattattcaa tcggttaacg ggaaaagcgg ttagccggac agctttcgtc gaacatcttg
    18841 gtcatgaagt tgtacaacat catccaaact ggaaagtcat gatttcgact gaccataaat
    18901 tgatgcgcat tgatgctcca ccaaacagcc attattgata cgcctccgtc gcctgttagg
    18961 tttatggtgt tttgccttgg cgacgctacg cttaatctgt tactgatttc tggtaccatg
    19021 gggggaataa tctgatttta tttgattgca aattaaccac ttaaacctat tgaatgcgga
    19081 acattgtcag tcggttaatt ttgcgtgctt tagcattcac atctttccgg acgatgcagt
    19141 gaaaattgcg taatcctcag caaccgctgc gtaatgtcgt ctatcttgtc gcgatcctgg
    19201 catccctaca ttatttgtgg tctgtgaaga ttatctcatt gcagcccctc atcttcgcag
    19261 ggctggcttt acagcttttc accctacgtt ataagaagtt ccgtcgatga tggcgctaat
    19321 ttcgtgaatt gtgcggcttg ttgcaaatta cacggtgttg aaggttattt acatgttagc
    19381 tgttgattat cttccctgat aagaccagta tttagctgcc aattgctacg aaatcgttat
    19441 aatgtgcgac ctcgtcctcc ctgacgcagt ttttgcgctg cggaaaaggt gacattggcg
    19501 caacgaaggt atattttgtt ttttgccgga ggatagcagc agatcgctgc acaatgtccg
    19561 tcaagtctaa cattgacact ctggggcaaa atagaccggc gtcccggcct gctggaattt
    19621 atcgctatgc atacagctgt cggggcatac gctttacaga cggcggtgaa acgcctgtca
    19681 caatcacact aaacaaagag tacggaaccc actcatggat attcgtaaga ttaaaaaact
    19741 gatcgagctg gttgaagaat caggcatctc cgaactggaa atttctgaag gcgaagagtc
    19801 agtacgcatt agccgtgcag ctcctgccgc aagtttccct gtgatgcaac aagcttacgc
    19861 tgcaccaatg atgcagcagc cagctcaatc taacgcagcc gctccggcga ccgttccttc
    19921 catggaagcg ccagcagcag cggaaatcag tggtcacatc gtacgttccc cgatggttgg
    19981 tactttctac cgcaccccaa gcccggacgc aaaagcgttc atcgaagtgg gtcagaaagt
    20041 caacgtgggc gataccctgt gcatcgttga agccatgaaa atgatgaacc agatcgaagc
    20101 ggacaaatcc ggtaccgtga aagcaattct ggtcgaaagt ggacaaccgg tagaatttga
    20161 cgagccgctg gtcgtcatcg agtaacgagg cgaacatgct ggataaaatt gttattgcca
    20221 accgcggcga gattgcattg cgtattcttc gtgcctgtaa agaactgggc atcaagactg
    20281 tcgctgtgca ctccagcgcg gatcgcgatc taaaacacgt attactggca gatgaaacgg
    20341 tctgtatcgg ccctgctccg tcagtaaaaa gttatctgaa catcccggca atcatcagcg
    20401 ccgctgaaat caccggcgca gtagcaatcc atccgggtta cggcttcctc tccgagaacg
    20461 ccaactttgc cgagcaggtt gaacgctccg gctttatctt catcggcccg aaagcagaaa
    20521 ccattcgcct gatgggcgac aaagtatccg caatcgcggc gatgaaaaaa gcgggcgttc
    20581 cttgcgtacc gggttctgac ggcccgctgg gcgacgatat ggataaaaac cgcgccattg
    20641 ctaaacgcat tggttatccg gtaattatca aagcctccgg cggcggcggc ggtcgcggta
    20701 tgcgcgtagt gcgcggcgac gctgaactgg cacaatccat ctccatgacc cgtgcggaag
    20761 cgaaagctgc tttcagcaac gatatggttt acatggagaa atacctggaa aatcctcgcc
    20821 acgtcgagat tcaggtactg gctgacggtc agggcaacgc tatctatctg gcggaacgtg
    20881 actgctccat gcagcgccgc caccagaaag tggtcgaaga agcaccagca ccgggcatta
    20941 ccccggaact gcgtcgctac atcggcgaac gttgcgctaa agcatgtgtt gatatcggct
    21001 atcgcggtgc gggtactttc gagttcctgt tcgaaaacgg cgagttctat ttcatcgaaa
    21061 tgaacacccg tattcaggta gaacacccgg ttactgaaat gatcaccggc gttgacctga
    21121 tcaaagaaca gctgcgtatc gctgccggtc aaccgctgtc gatcaagcaa gaagaagttc
    21181 acgttcgcgg ccatgcggtg gaatgccgta tcaacgccga agatccgaac accttcctgc
    21241 caagcccggg caaaatcacc cgtttccacg caccaggcgg ttttggcgtg cgttgggagt
    21301 ctcatatcta cgcgggctac accgtaccgc cgtactatga ctcaatgatc ggtaagctga
    21361 tttgctacgg tgaaaaccgt gacgtggcga ttgcccgcat gaagaatgcg ctgcaggagc
    21421 tgatcatcga cggtatcaaa accaacgttg atctgcagat ccgcatcatg aatgacgaga
    21481 acttccagca tggtggcact aacatccact atctggagaa aaaactcggt cttcaggaaa
    21541 aataagactg caaaagcgtc aaaaggccgg attttccggc cttttttatt actggggatc
    21601 gacaaccccc ataaggtaca atccccgctt tcttcaccca tcagggacaa aaaatggaca
    21661 ctcgttttgt tcaggcccat aaagaggcgc gctgggcgct ggggctgacc cttttgtatc
    21721 tggcggtatg gttagtagcc gcttacttac ctggcgttgc ccccggtttt accggttttc
    21781 cgcgctggtt tgagatggcc tgcatcctga cgccgctgct gtttattgga ctgtgctggg
    21841 cgatggtgaa atttatctat cgcgatatcc cactggagga tgacgatgca gcttgaagta
    21901 attctaccgc tggtcgccta tctggtggtg gtgttcggta tctctgttta tgcgatgcgt
    21961 aaacggagca ctggcacctt ccttaatgag tatttcctcg gcagccgctc tatgggcggt
    22021 attgtgctgg cgatgacgct caccgcgacc tatatcagtg ccagttcgtt tatcggcggg
    22081 cctggagctg cttataaata cggcctgggc tgggtattgc tggcgatgat tcagcttcct
    22141 gcagtctggc tttcgctcgg tattctcggc aagaagttcg caattcttgc gcgccgctac
    22201 aatgcggtga cactgaacga catgctgttt gcccgctacc agagtcgtct tttggtgtgg
    22261 ctggcgagtt tgagtttgct ggtcgcgttt attggcgcga tgaccgtgca gtttatcggc
    22321 ggcgcgcgcc tgctggaaac cgcggcgggt attccttatg aaaccgggct gctgattttc
    22381 ggtatcagca ttgcgttgta taccgccttt ggtggctttc gcgccagcgt gctgaacgac
    22441 actatgcaag ggctggtgat gctgattggc accattgtgc tgcttattgg cgtggtacat
    22501 gccgcaggtg gcttaagcaa cgcagtagag acgttgcaaa ccatcgatcc gcaactggtt
    22561 acgccacaag gcgctgacga tattctgtcg cctgccttta tgacgtcgtt ctgggtactg
    22621 gtgtgctttg gcgtgattgg cctgccgcat actgcggtgc gctgtatctc ttataaagac
    22681 agcaaagccg tacatcgggg gatcatcatc ggtacgattg tggtcgcaat cctgatgttc
    22741 ggtatgcacc tggccggagc gttaggtcgg acggtgatcc ccgatctcac cgtaccggac
    22801 ctggtgatcc cgacgttaat ggtaaaagtg ttgccaccgt ttgctgccgg gatcttcctg
    22861 gctgcaccta tggctgcgat catgtcgaca attaacgccc aactgctgca aagttccgct
    22921 acgatcatta aagatctcta tctgaatatc cgtccggatc aaatgcaaaa cgagacgcgt
    22981 ctgaagcgga tgtcggcggt aattacgtta gttctcggtg cgttgctgct gcttgccgcc
    23041 tggaagccgc cagaaatgat catctggctg aatttgctgg ccttcggtgg gctggaagcc
    23101 gttttcctgt ggccgctggt actgggtctt tactgggaac gcgccaacgc caaaggcgcg
    23161 ctaagtgcga tgattgttgg cggcgtactg tatgccgtac tcgcgacgct gaatattcag
    23221 tacctgggct tccaccctat cgtgccctcg ttactactaa gtttgctggc tttcctggtc
    23281 ggaaaccgtt tcggtacatc cgtcccgcaa gctaccgttt tgactactga taaataaaga
    23341 gttttgccat gccttggatc caactgaaac tgaacaccac cggcgcgaac gcggaagatc
    23401 ttagcgatgc gctgatggaa gcgggtgccg tttctatcac ttttcaggat acccacgata
    23461 cgcctgtgtt tgagccgctg ccgggcgaaa cgcgcctgtg gggcgacacc gatgtgattg
    23521 gtctgttcga cgctgaaacc gatatgaacg acgtggtggc gattctggaa aaccatccgc
    23581 tgctcggcgc gggcttcgcg cataaaatcg aacaactgga agataaagac tgggagcgcg
    23641 aatggatgga taatttccac ccgatgcgct ttggtgaacg gctgtggatc tgcccaagct
    23701 ggcgtgatgt gccggacgaa aacgccgtca acgtgatgtt agatccaggg ctggcgtttg
    23761 gtacgggtac ccatccaact acctctctgt gcctgcagtg gctcgacagc ctcgatttaa
    23821 ccggtaaaac agtcatcgac tttggctgcg gttccggcat tctggctatc gcggcgctga
    23881 aactgggtgc agcaaaagcc attggtattg atattgatcc gcaggcgatt caggccagcc
    23941 gcgacaacgc ggaacgtaat ggcgtctctg accgtctgga actctactta ccgaaagatc
    24001 agccagaaga aatgaaagcc gacgtggtgg tcgctaacat ccttgcaggc ccattacgtg
    24061 aactggcacc gttaatcagc gtcctgccag tttcaggcgg tttgctgggc ctttccggta
    24121 ttctggcaag ccaggcagag agcgtttgcg aagcttatgc cgacagcttc gctctggacc
    24181 cggtcgtgga aaaagaagag tggtgccgca ttaccggtcg taagaattaa ccttcgcatc
    24241 gccgtagggt gacgcagagc gaagtgcgag cgatgtcaca aaaggcacgt taatttgccg
    24301 attatttacg caaatttgcg tgccaaaatt ttcattcata aagaaaaatt gagaacttac
    24361 tcaaatttct tggagtgtaa attttagtca ctattttcta atatgatgat ttttatgagt
    24421 aattatcgcg ccacgctcat tttaaatgca attctttgat ccatctcaga ggattggtca
    24481 aagtttggcc tttcatctcg tgcaaaaaat gcgtaatata cgccgccttg cagtcacagt
    24541 atggtcattt cttaactcat gcgcatcgga caatatcagc tcagaaatcg cctgatcgca
    24601 gcgcccatgg ctggcattac agacagacct tttcggacgt tgtgctacga gatgggagcc
    24661 ggattgacag tatccgagat gatgtcttct aaccctcagg tttgggaaag cgacaaatct
    24721 cgtttacgga tggtgcacat tgatgaaccc ggtattcgca ccgtgcaaat tgctggtagc
    24781 gatccgaaag aaatggcaga tgcagcacgt attaacgtgg aaagcggtgc ccagattatt
    24841 gatatcaata tgggttgccc ggctaaaaaa gtgaatcgca agctcgcagg ttcagccctc
    24901 ttgcagtacc cggatgtcgt taaatcgatc cttaccgagg tcgtcaatgc agtggacgtt
    24961 cctgttaccc tgaaaattcg cactggctgg gcaccggaac accgtaactg cgaagagatt
    25021 gcccaactgg ctgaagactg tggcattcag gctctgacca ttcatggccg tacacgcgcc
    25081 tgtttgttca atggagaagc tgagtacgac agtattcggg cagttaagca gaaagtttcc
    25141 attccggtta tcgcgaatgg cgacattact gacccgctta aagccagagc tgtgctcgac
    25201 tatacagggg cggatgccct gatgataggc cgcgcagctc agggaagacc ctggatcttt
    25261 cgggaaatcc agcattatct ggacactggg gagttgctgc ccccgctgcc tttggcagag
    25321 gttaagcgct tgctttgcgc gcacgttcgg gaactgcatg acttttatgg tccggcaaaa
    25381 gggtaccgaa ttgcacgtaa acacgtttcc tggtatctcc aggaacacgc tccaaatgac
    25441 cagtttcggc gcacattcaa cgctattgag gatgccagcg aacagctgga ggcgttggag
    25501 gcatacttcg aaaattttgc gtaaacagaa ataaagagct gacagaacta tgttcgaaca
    25561 acgcgtaaat tctgacgtac tgaccgtttc taccgttaac tctcaggatc aggtaaccca
    25621 aaaacccctg cgtgactcgg ttaaacaggc actgaagaac tattttgctc aactgaatgg
    25681 tcaggatgtg aatgacctct atgagctggt actggctgaa gtagaacagc ccctgttgga
    25741 catggtgatg caatacaccc gtggtaacca gacccgtgct gcgctgatga tgggcatcaa
    25801 ccgtggtacg ctgcgtaaaa aattgaaaaa atacggcatg aactaattca ggttagctaa
    25861 atgcttgatt aaaaaggcgc tactcggcat ggggaagcgc cttttttata ggtgtcacaa
    25921 agggagtgac catgagaaca ggatgtgaac cgacccgatt tggtaatgaa gcaaagacca
    25981 tcatccaggg tgatgcatta accgaactta aaaagctccc cgccgaaagc gtcgatctga
    26041 tctttgccga cccaccgtat aacatcggta aaaattttga tggtctgatc gaagcctgga
    26101 aagaagatct gtttatcgac tggctgtttg aagtgattgc agagtgccac cgcgttctga
    26161 aaaagcaggg cagcatgtac atcatgaaca gcacggaaaa catgcccttt atcgatctcc
    26221 agtgccgcaa gctttttacc atcaaaagtc gcatcgtctg gtcatatgac agttcaggag
    26281 tgcaggcgaa aaaacactac ggctccatgt acgaacccat cctgatgatg gtgaaagacg
    26341 caaagaacta cacattcaac ggtgatgctg ttctggtaga agccaaaacc ggatcgcagc
    26401 gcgcgttgat cgattatcgc aaaaatcctc cacagcctta caatcatcaa aaagtaccgg
    26461 gtaacgtctg ggattttcct cgcgtgcgtt atttgatgga tgaatatgaa aaccacccga
    26521 cgcaaaaacc ggaagcctta ctgaaacgca ttattctcgc ctcttccaac ccaggcgata
    26581 tcgttctcga cccgtttgcc ggtagcttta ctaccggtgc cgtagccgtc gccaacgggc
    26641 gaaaattcat tggtattgag atcaacagcg agtacatcaa aatggggctt cgacggctgg
    26701 atgtcgcgtc gcattactct gcggaagaac tggcgaaagt gaaaaaaaga aagaccggca
    26761 acctgtcaaa acgaagccgg ttaagcgaag ttgaccccga tctcattgca aagtaaagga
    26821 agtgtaagcc tggttttcag attattcatt tcgtgtatat ttcctgccag acttggttaa
    26881 acatgcacag gctcaggtaa tgattcgcaa gtattggtgg ctcgtcgttt tcgctgtctt
    26941 cgtttttctg tttgatactt tactgatgca gtggattgaa ctgctggcaa cagaaacaga
    27001 caaatgccgc aatatgaact cagttaatcc actaaaactg gttaactgtg acgaactgaa
    27061 ttttcaggac agaatgtgaa tttatatgac acttaattca ttcgttttat gaattaattt
    27121 cgttatgttt tcatctggca tgaacattct taatacgtta tcgaccagag cgggggcttg
    27181 tttataaaga tcataacccg ccatattcat taaccagttt tgaacaattc cgctgaaggc
    27241 accatcaata ataatcatca caacatctaa atcgagatta tttgctacac aaccttgttg
    27301 ctgacacgcc tgcaatactt cgcggagagt ctgcggatta aagcccatct tttcgcgtat
    27361 cactccctcg gccagcatct catcattaaa ttcacatttg tgatataaga ttttcagcaa
    27421 cgcctgctgg cggggaattt tggcaatata ttgcaaaccg acaatcaatt tttcacgcaa
    27481 ttgctgaaac gggtcatgct ctaacccagc ggtcaagtga tcctggatta actcccgcaa
    27541 tgaaggctgt tgcaaccaca tctcattaaa cagttgggtc ttgttctcga agtgccagta
    27601 gatagcgcca cgcgtaacgt tagcagcgtc ggcaatgtcg ttgagcgtcg tcttgcttac
    27661 gccatgttgc gcaaactggg cgatggcagt ttcaatcagt tcttgccggg tcttcagagc
    27721 ttcggctttg gttctttttg ccatgattaa ttattcagga aataaatata ttcgacacag
    27781 agtgagaaaa tagcgaaggt taatctatca cctaatgtgt
//
LOCUS       DAZGHF010000059        27792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGHF010000059 DAZGHF010000000
VERSION     DAZGHF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.059"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..191)
                     /locus_tag="ACHK3A_002574"
     CDS             complement(<1..191)
                     /locus_tag="ACHK3A_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740633.1"
                     /translation="MDIVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGAVT
                     VKNGIWQAIAGGVGNKTNIA"
     gene            complement(227..439)
                     /locus_tag="ACHK3A_002575"
     CDS             complement(227..439)
                     /locus_tag="ACHK3A_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO8740634.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEGDTSPTE"
     gene            complement(399..1025)
                     /locus_tag="ACHK3A_002576"
     CDS             complement(399..1025)
                     /locus_tag="ACHK3A_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFO8740635.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            complement(1022..1453)
                     /locus_tag="ACHK3A_002577"
     CDS             complement(1022..1453)
                     /locus_tag="ACHK3A_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO8740636.1"
                     /translation="MKQIAFYRRSGRPGAFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARGLTKGSKVVKIKASEPFTTDTGIVDRFFSLESNPRRNTPRSRNAVPP
                     FSRRSREHAAKNCREEYVQKAERRRRLIKAGLYIDEFENAL"
     gene            complement(1509..2180)
                     /locus_tag="ACHK3A_002578"
     CDS             complement(1509..2180)
                     /locus_tag="ACHK3A_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO8740637.1"
                     /translation="MTTQISVETISPITHNQIPVITTELLAQLYGTETNNIKVNYTRN
                     AERFVCGKHYFKLEGAGLREFKNKVTQSNLVAPRTKHLILWTERGAARHAKMLETDQA
                     WEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSEHHPYSMMSYVDRKTLNLDVS
                     VLFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKG
                     FSLSL"
     gene            2508..2765
                     /locus_tag="ACHK3A_002579"
     CDS             2508..2765
                     /locus_tag="ACHK3A_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO8740638.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAMEAWLIHSAAPPLDAIRENLGKGHSISQVRSEIRARK"
     gene            2819..3091
                     /locus_tag="ACHK3A_002580"
     CDS             2819..3091
                     /locus_tag="ACHK3A_002580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740639.1"
                     /translation="MSLAAPIRDPIMNIIARFFYLIIFCMATSGCTTTTKINRGDEKE
                     QYIIACGAATPWGVCYDKANALCKNGYKDILKEQGFNRKELTIECK"
     gene            3181..3486
                     /locus_tag="ACHK3A_002581"
     CDS             3181..3486
                     /locus_tag="ACHK3A_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO8740640.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NTTISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(3529..4269)
                     /locus_tag="ACHK3A_002582"
     CDS             complement(3529..4269)
                     /locus_tag="ACHK3A_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740641.1"
                     /translation="MINNIANQSSFPAATIDSQMLLKMVNEARKLCSEKPVRNNDFIA
                     RVKDELEGETYEIFVGQKNGAEIEVITMTFKQALRVAARESKAVRRSLVDQLESMQQQ
                     LQQKITSKHSTNGLEEFRKARALKMTVDTMKDLFGFLPNLAPEAKQVVAASLVNPVVG
                     ANVIPLPMINEHYYSASEVGAKLKISANKVGRIANTYMLKTEQYGKWFIDKSPYSDKQ
                     VESFRYNNRGVQKIEEILDAENKAEFGT"
     gene            complement(4318..4602)
                     /locus_tag="ACHK3A_002583"
     CDS             complement(4318..4602)
                     /locus_tag="ACHK3A_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO8740642.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWINRLVNRKYDRL
                     IITKGYPKRDDSSRRIDTPYDGYKIKTITHTHFGDKPVKVFAIKVNIGSD"
     gene            complement(4595..5128)
                     /locus_tag="ACHK3A_002584"
     CDS             complement(4595..5128)
                     /locus_tag="ACHK3A_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO8740643.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALTSLE
                     AEPVAWKVTFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAKPVPVT
                     PDGWISCSERMPEKGQNVLISVNFDSSLVEPLICSARYTGSTFRRGDATIKPGNGIEQ
                     ATHWMPLPEPPQEVNRG"
     gene            complement(5125..5490)
                     /locus_tag="ACHK3A_002585"
     CDS             complement(5125..5490)
                     /locus_tag="ACHK3A_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO8740644.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATV"
     gene            complement(5492..6352)
                     /locus_tag="ACHK3A_002586"
     CDS             complement(5492..6352)
                     /locus_tag="ACHK3A_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO8740645.1"
                     /translation="MSEIDYQALREKAEKATCGEWSLEYGEGRFDGDDALIHREAAGY
                     IPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDERERNQQYIKRRDQE
                     NEEIALTVGRLLIENGQLVADTLRHLADNEIDSDYFAITSTNENGTEIEHEMAITDYA
                     LQAAGTVDELVAALEAAEKRNAKLQRENAYIRNRYKELDLLIGKNILVMQAAIIEWQA
                     TGDAKSGLAWIYNTLFGPGELPDKSEKDAQAYFNRKYAPIDEKLMALHKWFWEQSEAE
                     RSAGIRIKGE"
     gene            complement(6349..6834)
                     /locus_tag="ACHK3A_002587"
     CDS             complement(6349..6834)
                     /locus_tag="ACHK3A_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFO8740646.1"
                     /translation="MTGHAAILDMCCGSRMFWFDKNDDRAIFSDIRKEEHTLCDGRRL
                     IISPDLIADFRALPFADASFPVVVFDPPHLERVGDNAWMGKKYGRLNKDTWRDDLRQG
                     FKEAFRVLWPYGVLIFKWNETQIPVRQILALTDRKPVIGQRTGKGDKTHWIIFMKEGN
                     Q"
     gene            complement(6831..7532)
                     /locus_tag="ACHK3A_002588"
     CDS             complement(6831..7532)
                     /locus_tag="ACHK3A_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO8740647.1"
                     /translation="MTTEIDYQVLRDVSERAITAMTHLSILPGDDDLLSEKKLKELGI
                     DIDAIHAFKIMAGPETVLSLLDERDALNERIAELEANLAKLAEDQQKAIESIKQADSA
                     VKLAHEKFSELAAENAALKQSEKEFNNFCRQEYYGWEDNFTETPATDAFLAEIRAEAR
                     NEGINYTASRLAAAFNHGFINKSLREVFDVTRMILSAKEELANEPYPIDGLSGEYAEK
                     SLEEWAERLRKGGNQ"
     gene            complement(7635..8078)
                     /locus_tag="ACHK3A_002589"
     CDS             complement(7635..8078)
                     /locus_tag="ACHK3A_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740648.1"
                     /translation="MQFKDLPEDIQKIAADTLKAHLSVLDLTKEPKANLENISRNVRD
                     VFVGLYAYDNEKHEEHIQNGCINKFQQNIKLPVADKTEQKTDVLVFWNTVEAIARSTM
                     LEFNNINHSGIDTHQSSHREGFSILLTLLQEQGEYPQPTSNDTEQ"
     gene            complement(8155..8976)
                     /locus_tag="ACHK3A_002590"
     CDS             complement(8155..8976)
                     /locus_tag="ACHK3A_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO8740649.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADECSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKRQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAVRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLGNRE
                     FTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEEEDILTFIGTFSV"
     gene            complement(9040..9387)
                     /locus_tag="ACHK3A_002591"
     CDS             complement(9040..9387)
                     /locus_tag="ACHK3A_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740650.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            complement(9462..10049)
                     /locus_tag="ACHK3A_002592"
     CDS             complement(9462..10049)
                     /locus_tag="ACHK3A_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFO8740651.1"
                     /translation="MDEVIFTYNEESALTAGQGSFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTGIGQLTQHMVSVSK
                     YVAPEFHGKKIGLVLQKVLTTNRKTGADGYQMEIRIPFIAETGQTLKEKAEGKKPETV
                     ANMVSTLKDKDNRSKNVNPNHTDAPGYWQYGSDSF"
     gene            complement(10049..10738)
                     /locus_tag="ACHK3A_002593"
     CDS             complement(10049..10738)
                     /locus_tag="ACHK3A_002593"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO8740652.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKMSYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVSE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFDAIKPAYMAQVQF
                     SMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEALAEIG
                     FVFGEQWGVNN"
     gene            complement(10735..11685)
                     /locus_tag="ACHK3A_002594"
     CDS             complement(10735..11685)
                     /locus_tag="ACHK3A_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO8740653.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNAFLA
                     DTARANLPSAQNAIINVAAIGITLNPASKLAYLVPRKKAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGNNGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLNTQG
                     GEGIILNADHIPERDVTPASDEIINEITQAITEINKTWDDLLPLCSKTFRRTIASHEY
                     LSQEEAVKTLDFVKKKAARNKATAEAKIHATTENNSEAVS"
     gene            complement(11702..11983)
                     /gene="gamL"
                     /locus_tag="ACHK3A_002595"
     CDS             complement(11702..11983)
                     /gene="gamL"
                     /locus_tag="ACHK3A_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HFO8740654.1"
                     /translation="MNAYLTCDRIEERRWINQQIQDEKDKWIDDRAQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            complement(12004..12285)
                     /locus_tag="ACHK3A_002596"
     CDS             complement(12004..12285)
                     /locus_tag="ACHK3A_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO8740655.1"
                     /translation="MLNQKININVKSVVTPAGTIMGEVFMDDKIIAYFVILSDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            complement(12297..12509)
                     /locus_tag="ACHK3A_002597"
     CDS             complement(12297..12509)
                     /locus_tag="ACHK3A_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO8740656.1"
                     /translation="MLKPHYGTALVSREDVKPGTAILYNGRYYMASANVNNALYAHSL
                     IEKIRIISDAIEVYLNNKGQPLISPA"
     gene            complement(12580..13353)
                     /locus_tag="ACHK3A_002598"
     CDS             complement(12580..13353)
                     /locus_tag="ACHK3A_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO8740657.1"
                     /translation="MGPTSVGLVPLIASSPNPVQSTASELRTSCGGSQSPIKEAAIMA
                     TIPALSQPEITITNGRVVTTSIAIAEFFDKRHDDVLKKIRLMDCSTEFNARNFADVTY
                     TDAKGEQRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKQLWEQKQLALSS
                     PAMNINFPLSWFSEHHPYATMNYVDRKVISLDASVLFDMPSPTMRILNELHSKGYNVD
                     AAVAEFNAFKHLTEEMRRKLLDISRTSEKSSRFGFNVNL"
     gene            complement(13982..14935)
                     /locus_tag="ACHK3A_002599"
     CDS             complement(13982..14935)
                     /locus_tag="ACHK3A_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFO8740658.1"
                     /translation="MNNQNDYIEAAQQIIASLGLPRAQQNERSALCLLALLNLTPGKA
                     WADAENPLVGITPIMNWVREHYGKVYAPNTRETFRRQSMHQFCAAGVALYNPDKPDRP
                     VNSPKAVYQIEPAALSMLRTFGSPAWHDSLATYLAERETLVTRYAKEREQNRIPVEIA
                     AGQQITLSPGEHSELIRAIIEDFAPRFAPGSVLVYAGDTGEKWGYFDAPLLAGLGVDV
                     DSHGKMPDVVLHFTAKNWLLLVESVTSHGPVDGKRHAELARLFAGSTAGLVYVTAFPN
                     RSIMGRYLGEIAWETEVWVADAPSHLIHFNGERFLGPYSTE"
     gene            complement(14932..16401)
                     /locus_tag="ACHK3A_002600"
     CDS             complement(14932..16401)
                     /locus_tag="ACHK3A_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFO8740659.1"
                     /translation="MLQKLDVADSVRRELSPRTARKHKAEFGQFMTPSGVARFMASLF
                     PPSTMKTCRLLDAGAGVGALSCAFLDRWLVGEFGFESVEATAYEIDKKLCGHLAKHLT
                     GYRRVTPRIIEGDYIELATAEGLQDRGYTHAILNPPYKKINSQSAHRQALRTVGIETV
                     NLYSAFVALAVGEVAPGGQIVAIIPRSFCNGPYYRPFRNFILERAAIRHIHLFESRSK
                     AFRDDDVLQENIIIRLERGGLQEAVTVTTSTDDSFFDLVTYEHPFDQIVYPDDSERFI
                     HVPTTHEKSAIELSSAVQCSLADIGVKVSTGPIVDFRLKAHLRSMPEEGTVPLIYPSH
                     LSMSSTVWPVEGLKKPNAIMRNDETEKWLYPNGFYCVVRRFSSKEEKRRVVASVVDPA
                     TFSEYSVLGFENHMNVFHENKHGLPEALARGLAVFLNTTAVDKYFRRFNGHTQVNATD
                     LKMIKYPSRDTLIELGKWAMQQETLTQEHIDAKLGALTA"
     gene            complement(16496..17209)
                     /locus_tag="ACHK3A_002601"
     CDS             complement(16496..17209)
                     /locus_tag="ACHK3A_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO8740660.1"
                     /translation="MNMKKKPLTPEQLEDAKRLKSIFNAKKKELGLSQESLAYELGVT
                     QSAVNQLMAGINAINASHAAQLAKILNVKVGDFSPSLAKSIAEMALAIEEPLTRVPAY
                     EYPLLSCVQAGAFSMDDISYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGKPS
                     FPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIECNE
                     NCQFVGKVIKSQWNDETFD"
     gene            17305..17508
                     /locus_tag="ACHK3A_002602"
     CDS             17305..17508
                     /locus_tag="ACHK3A_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO8740661.1"
                     /translation="MAMKKETLADYVYRFGQKKAAKDFGVAQSAISKALLVGREIYVK
                     TFDDGRVEAEEVRPFPAFVRGDD"
     gene            17563..17832
                     /locus_tag="ACHK3A_002603"
     CDS             17563..17832
                     /locus_tag="ACHK3A_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740662.1"
                     /translation="MPEQYCDADRDWIQEQLLQLDPTTRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMLSPVAVRKFPPTP"
     gene            18040..18417
                     /locus_tag="ACHK3A_002604"
     CDS             18040..18417
                     /locus_tag="ACHK3A_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740663.1"
                     /translation="MSTATKSTLIRTLSTVPLRTEERYSFLADVVTLLESQGMHVASN
                     VTVRIDGRNFRVDILATAKTGGSVAIEIDRSSPRSRSVMKLRELARRGTEGFVLLRMP
                     KKLTSYSDAGIDIIPANGKGASC"
     gene            18411..19931
                     /locus_tag="ACHK3A_002605"
     CDS             18411..19931
                     /locus_tag="ACHK3A_002605"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO8740664.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDRDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVRNWLENGEDLPTICFCVNVAHANFVTREFLQAGIGAEVMTADTLHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTAPGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGSEVKAEKLPRECSKCHFMKPAGVHMC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYSREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVHNFIRSKIIAYAKGRKKAMTGGQHAD"
     gene            19921..20892
                     /locus_tag="ACHK3A_002606"
     CDS             19921..20892
                     /locus_tag="ACHK3A_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO8740665.1"
                     /translation="MQTKKAAVGQWGRIFEYYGMPPVTGVKHYNGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMKLLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRELVMDKFAGLPCLRGTTGEAYLQGRGILQLPTESVRFCDRQIASGREYQAIYAIA
                     TDDRGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDEHGAGLAAAFKCG
                     HKNLMSRNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            20892..21341
                     /locus_tag="ACHK3A_002607"
     CDS             20892..21341
                     /locus_tag="ACHK3A_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO8740666.1"
                     /translation="MEIEMLKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGCSNNPELNNRLLSFF"
     gene            21349..21912
                     /locus_tag="ACHK3A_002608"
     CDS             21349..21912
                     /locus_tag="ACHK3A_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO8740667.1"
                     /translation="MKQRKPKKCKVCGSSFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRHLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHSRHRW
                     TVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            21909..22103
                     /locus_tag="ACHK3A_002609"
     CDS             21909..22103
                     /locus_tag="ACHK3A_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO8740668.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            22096..22530
                     /locus_tag="ACHK3A_002610"
     CDS             22096..22530
                     /locus_tag="ACHK3A_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO8740669.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKRLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            23037..23984
                     /gene="stxA1a"
                     /locus_tag="ACHK3A_002611"
     CDS             23037..23984
                     /gene="stxA1a"
                     /locus_tag="ACHK3A_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO8740670.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            23994..24263
                     /gene="stxB1a"
                     /locus_tag="ACHK3A_002612"
     CDS             23994..24263
                     /gene="stxB1a"
                     /locus_tag="ACHK3A_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO8740671.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            24767..26734
                     /locus_tag="ACHK3A_002613"
     CDS             24767..26734
                     /locus_tag="ACHK3A_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO8740672.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGELPSLAESGNHPHVSAKPEAAPEYYYVIALAGQSNSMSYGEGLPLPET
                     YDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYG
                     TVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVD
                     KPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLA
                     DMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDEN
                     GVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSTWARRGIISDRLATAI
                     LVHAGRTAEFITGKQPDMVRPTVPSGEGPEREAEAPASKRTLMSLLASGEDLASQGWR
                     YYHKPAGGDSVNKNIAEAVVSDAGATGGKALQLNKPENHIWFLEHDAAGQGAELLKKG
                     GRVSVRFKLPGSLVPNQFALGIYWQLSSLPEGVMLSGEGNDMLMSFFLQTDTTNLNAM
                     HHRKPNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGAAFTLVKSPASGAAD
                     KLQLTDISKSATYTLLIDSIAVEVNSTDTAA"
     gene            26869..27048
                     /locus_tag="ACHK3A_002614"
     CDS             26869..27048
                     /locus_tag="ACHK3A_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8740673.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            27089..27334
                     /locus_tag="ACHK3A_002615"
     CDS             27089..27334
                     /locus_tag="ACHK3A_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO8740674.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            27412..27627
                     /locus_tag="ACHK3A_002616"
     CDS             27412..27627
                     /locus_tag="ACHK3A_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8740675.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            27632..>27792
                     /locus_tag="ACHK3A_002617"
     CDS             27632..>27792
                     /locus_tag="ACHK3A_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8740676.1"
                     /translation="MSRKLRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRG"
BASE COUNT         6822 a         6464 c         7041 g         7465 t
ORIGIN      
        1 gcgatatttg ttttgtttcc aacgccccct gcaattgctt gccagatgcc gttttttacc
       61 gttacggccc cctggttttt taacttccac agcgtatcaa tgacgtcatt caaatcaata
      121 ttcagacctt tagaaatact ttcagctgat gcttttccta atttgttcaa ttctgtaagt
      181 actatatcca tttttttctc ctgatatttt gatgtcggtg gtgctgttat tcggttggag
      241 aggtgtcacc ttcttctacc agcttttcca gttcatccag ctttcgggaa agaatttcac
      301 caaacagatg aagttctgca tctgctgtaa tcgggattgg aacaaatcgt attccacttc
      361 tggcaagttg atttgcgatt tctagacact gccttaattc aactggtgat gcctttgtca
      421 acgtcgtttt ttcgctcatt ggttttctcc agaaatttaa caatacccgg aaccatctca
      481 atggatggtg tgccgcattg gttaccccat acatcaaatc catgcgaggt gtggcgggcg
      541 aacagttcta tccggggaac atcgccaagc agttgcacaa gcttttctcg agccatatcc
      601 ggcttgcggg agtgatcaag gcgtggtgca gtaaatgact ggattattcc ggcgttaatg
      661 cgttcgggca gatttccttt cactgcaaaa aggcagtctt cgctgttagc gcgagtggtg
      721 ctacccatac ccatgaccag cttgtcggtc tgtcttttcc cgcatttgtt ccaggttaat
      781 cctttcatcg tcaccagacg aaagccccac gcttctacaa ccttcaatgc ttcaagtggt
      841 tgtgtaggca cccaccacat agccaacagg caattatcgg ctgccagttc ccataccggg
      901 aggcggcaga tatccagaag actcatgacc ggatatttaa aacttgcacc gcgttgacca
      961 tcggttgctt tgtcgcggaa tgtccagggg ggatctgcgt aaatgagggt gtattttttc
     1021 gtcatagcgc gttttcaaac tcatcaatgt aaagtcctgc tttaatcagt cggcgacggc
     1081 gttcggcctt ttgcacgtat tcctcgcgac agttttttgc tgcgtgttca cggcttctgc
     1141 gactgaatgg aggaacggca ttgcgtgagc gaggtgtgtt acgacgagga tttgattcga
     1201 gggagaaaaa gcgatcaacg attccggtgt ctgtggtgaa tggttctgaa gcctttattt
     1261 tcacaacctt actgcccttt gtcaggccgc gagctacctt gttgaactcg caaagtgaaa
     1321 caccaaattt ctctgctatt tcactgccag taacaggacg accacgagac tgaatcatcc
     1381 aggtgacgcg ttcttttaac ccacggaagg caccgggcct tccagaacgc ctgtagaaag
     1441 cgatctgttt caaatttttg tctccagata tgaaaacccc gacgggatgt cggggatggt
     1501 aggggaagtt acaggcttaa cgagaagcct tttcgggaat ttcgatctga cagtcttgaa
     1561 atatcctgta gcgtgcggcg catttcttcc gtcagatgtt tgaaggcgtt aaattcggcg
     1621 acagcggcgt caacgttata gcccttgctg tgtagctcgt tgaggatgcg catagttggg
     1681 cttggcatat cgaagagcac agaaacgtcc aggttaagag tttttcgatc aacatagctc
     1741 atcatggagt atgggtgatg ctctgaaaac cacgatagag ggtagttgat agatagtgtt
     1801 gattctggaa gctgaagttg ttctgggatt ttttgactga aatagcagtc ttccagtttt
     1861 tcgaacactt cccatgcctg atcggtttcg agcatttttg catgacgggc agcgccgcgt
     1921 tctgtccaga ggatgaggtg ctttgtacgc ggtgcaacta agttactttg agtaaccttg
     1981 ttcttaaatt cccgcaatcc agccccttcc aatttgaagt agtgtttccc acaaacaaaa
     2041 cgctcggcat tgcgtgtata gttcactttg atgttattag tttcggtgcc ataaagttgt
     2101 gccaaaagct cggtggtaat aacaggaatc tggttatggg taatcgggga gatggtttca
     2161 acagagattt gagttgtcat aatgacgccc tctagtggtt tctaaactat caccaccgta
     2221 aggttccaat catcgggtgg tgagacgcac agggttggaa ctaccgggaa accgaccggc
     2281 gagcttttca gctcccccat gcgccccacc ataattcaga tgtgcgcgtg catacgacaa
     2341 taaaaaacac gctcgcggcg tgtgtttgtc gcggtctcta tccggggttc caatcccgac
     2401 gccagatttt gctggcgtgc gaagaatata atcccggata tgtgttgtcg tcaacaagtg
     2461 gcgtgctatc atcgaatagt gttctatcct actcagtgag gtttaacatg cgtacaaccc
     2521 aacaattcag cattacatta accaacgaaa tggctgacat ggtgcgcgcc cgtgtggctt
     2581 ccggtgcata cgcttcagaa agcgaagtta ttcgtgaagg gcttcgtgca ctgaatgaac
     2641 gcgacaaagc aatggaagcg tggttaatac actcagccgc gccgccactc gacgctattc
     2701 gtgagaacct aggcaaaggg cactccattt cacaggttcg ctctgagatt cgcgcccgga
     2761 agtaatctgc atggcatatg aagtgccaca ctaacaaact gctctaggta tgggaatagt
     2821 gtcacttgca gcacctatta gagatccgat aatgaacatc atagcaaggt tcttttattt
     2881 gataattttc tgcatggcaa catcaggatg cactaccact acgaaaataa atcgcggaga
     2941 tgaaaaggaa cagtacatca ttgcctgtgg tgccgctaca ccatggggag tatgctatga
     3001 taaagccaat gccctatgta aaaatggata taaagacatt ttaaaggaac agggatttaa
     3061 caggaaagaa ttaaccattg agtgtaaata aggccatcat ttgcaacgtt gaaaaaattc
     3121 gcccgtgcta cagggaaaaa actccagagc cgcttcgttt aactacgagg attaccacta
     3181 atggcgatgt ttaacccccc tcatcccgga gaaattattg ctgatattct ggaagatcag
     3241 aatattggaa tcagggaact ggcaagagcg cttgatgtcg cgccttccac tgttcagcga
     3301 ctggtttcag gtaatacgac aatatctcct gaaatggctg ttcgtctcgc tgctgtcctg
     3361 ggtggaactc cgtcttcatg gattcgtctc cagacggcat ggagccttga aaaagcggaa
     3421 agagaagttg acgtatctca tctctcaaca aaataccgcc cggcagaaat ttcgcctcat
     3481 gcttaaccac cgcgccgtca ttctggcggc gtcggagagt ggaggatatc aggttccgaa
     3541 ctctgctttg ttctccgcgt ccaggatttc ttcaatcttc tgtactcccc gattattgta
     3601 tcggaagctt tctacctgct tatccgaata cggggatttg tcgatgaacc atttcccgta
     3661 ttgttcggtt ttgagcatgt aggtattggc aatgcgacca accttgttag cggatatttt
     3721 gagttttgct ccaacttctg acgccgaata gtaatgttca ttgatcatcg gtagcgggat
     3781 tacgttagcg cctacaacgg gattaaccag gctggcggca acgacttgtt tagcttcagg
     3841 ggcaaggttg gggaggaagc cgaaaaggtc tttcattgta tcgacggtca ttttcagcgc
     3901 ccgtgcttta cggaattcct caaggccgtt agtcgaatgt ttcgaggtga ttttttgttg
     3961 cagttgctgt tgcattgact ccagttgatc gaccagcgaa cgacggacgg ccttcgactc
     4021 acgcgcggct actcgcaggg cttgcttgaa agtcattgtt ataacttcta tttctgcgcc
     4081 gtttttctga cctacgaaaa tttcgtaggt ctctccttca agctcatcct taactctggc
     4141 aatgaaatca ttattgcgaa cgggcttttc actacataat ttccgtgcct cattgaccat
     4201 tttcaacagc atctggctgt cgattgttgc agccggaaac gaagactgat ttgcaatatt
     4261 gttaatcatg tattctgtcc ttttcagata aaagaaatcc ccgcgagtgc gaggatgtca
     4321 atcactgccg atattcacct ttattgcgaa cacctttacc ggtttatctc cgaagtgtgt
     4381 atgtgtgatt gtcttgattt tatatccgtc atacggggtg tctattctgc ggctggaatc
     4441 gtcgcgcttc ggatatccct ttgtgataat caggcggtca tacttacggt taacgaggcg
     4501 atttatccag tagtcattac acaggcgata ctcttccgtt ttctctccgc gaatcatggc
     4561 atcgaagtat tcacctttga cggcaagttg caggttagcc acggttaacc tcctgcggcg
     4621 gttctggtag cggcatccag tgagttgctt gctcaatacc attacccggc ttaatcgttg
     4681 catctccgcg ccgaaaggtg cttccggtat agcgtgcgga gcatattagc ggttcaacca
     4741 gagagctatc gaaattcacc gaaataagca cgttctggcc cttttcaggc attcgctcac
     4801 tacagcttat ccaaccatcc ggagttaccg gcactggctt ggcggtataa agcggtgtta
     4861 tatctgcccg aaaattacat gctttatgca gccgcaccca ccgttcgact tctgctttgt
     4921 cagaatacat accagtgaac gtgttatatt cacggtcaat ttgcgtgaat gttaccttcc
     4981 acgccaccgg ctctgcttcc agcgatgtca gtgcaatccg cgccagttct tccgcttctt
     5041 ctgctggcag tacaacgttg ctacccggtc cgtatgtttc gcgccactgc ttgattgtaa
     5101 gtagtcgctc tttggtaata gtggtcatac cgtagcccct tcttgatatt tttcaaacca
     5161 gaacacaacc gggtcagatt tcatttcaac caatcccata cgaaccagcg ccttgccttt
     5221 cccggacgca aggaattcac gacgaccatc actgataatt cgccgataat cttccaggct
     5281 actgcaatgc ttgtgcagat tgcatgggtg gcatgccgga accatgttgg atatatcgtc
     5341 acgttcctgg tgaagcatat ttccatcaaa acgaatgacc ggttttacat ggtctgcatg
     5401 ccacttttcg ccaagttcac agccgcaata agcacagcga ccgccaaact tcatgcgcaa
     5461 ttctgcacgt tgttttttcg tcagtgccat ttcactctcc tttgatgcga atgccagcgg
     5521 agcgctcggc ttcactttgt tcccaaaacc acttgtgaag cgccataagc ttttcgtcaa
     5581 tcggtgcata tttgcgatta aagtaggcct gagcatcttt ctcagatttg tccggtaatt
     5641 cgccagggcc aaacagtgtg ttataaatcc atgccagtcc gctcttagcg tcgccagttg
     5701 cctgccattc gataatggca gcctgcatga ccagaatgtt tttcccgatt aacaggtcca
     5761 gttctttgta ccggttgcgg atgtatgcat tctctctttg taattttgcg ttgcgcttct
     5821 ctgcggcttc cagcgccgca accaattcgt ctacagttcc ggcagcttgc agtgcgtaat
     5881 cggtaatagc catctcatgc tcaatttcag taccgttctc attcgttgag gtgatagcaa
     5941 aataatcaga gtcgatttcg ttatcagcta agtggcgtag cgtatcggca acaagctggc
     6001 cgttttcgat tagcagcctc cctaccgtta gcgcaatttc ctcgttctcc tggtcgcggc
     6061 gtttgatgta ttgctggttt ctttcccgtt catccagcag cgccaagacg gtagccggat
     6121 tggctgcggc gatgaattca gcattggcct gctgttccat ttggaaatct tcatcgaaac
     6181 cgctttcagg atgcgctcct tcaattctgc aaatgggaat atatccagca gcctcgcgat
     6241 gaattagtgc atcatcacca tcaaatcggc cctctccata ttcgagcgac cactcgccac
     6301 acgttgcttt ttctgccttt tcacgcagtg cctgatagtc aatctcgctc attggttgcc
     6361 ctccttcata aaaataatcc agtgggtctt gtcacccttt cctgttcgtt gaccgataac
     6421 aggctttctg tcggtcagtg ccaatatttg gcgaacaggt atttgcgttt cattccattt
     6481 aaaaatcaga acgccgtatg gccacaacac acgaaaggct tctttaaatc cctgccgcaa
     6541 atcatcacgc caggtatctt tattcagccg tccatatttc tttcccatcc aggcgttatc
     6601 accaacacgc tcaagatgcg gagggtcgaa tacaacaacc ggaaacgatg cgtctgcaaa
     6661 tggtaatgca cgaaaatctg ctatcaggtc agggctaatt atcagccgtc gtccatcaca
     6721 caatgtgtgc tcttcctttc tgatatcgct aaatatcgcc cggtcgtcat tcttatcgaa
     6781 ccagaacatg cgactgccac agcacatgtc gaggattgca gcatgtccag tcactggttg
     6841 cctcctttgc gaagccgttc cgcccattct tcaagggatt tctccgcata ttcaccggac
     6901 aggccatcaa tcgggtacgg ttcattagcc aactcttctt tcgctgacag aatcatgcgc
     6961 gtaacgtcga aaacttcacg caaagactta ttgataaatc cgtgattgaa cgcagcagca
     7021 agacggctgg cggtatagtt aatcccctcg ttgcgtgctt ccgcacgaat ttcagccaga
     7081 aaagcatcgg tggctggggt ttccgtgaag ttgtcctccc aaccgtagta ctcctgacga
     7141 cagaagttat taaattcctt ctccgactgt ttaagcgccg cattctccgc tgccagctcc
     7201 gaaaacttct cgtgtgccaa cttaacagcc gaatcagcct gcttaattga ctcaatcgct
     7261 ttctgttggt cttcggccag cttcgctaaa ttagcctcca gttcggctat gcgttcgttt
     7321 aatgcatctc gttcatccag tagtgacagc acggtttctg gcccggccat aattttaaag
     7381 gcgtgaatcg catcaatatc aataccaagc tccttgagct tcttttcgct taataaatca
     7441 tcatcacctg gtaatattga cagatgtgtc atcgctgtaa ttgcgcgttc cgacacgtca
     7501 cgcaatacct gatagtcaat ttcggttgtc atattttcgt ctcgttatgg gaggggaatt
     7561 ggttgtaagc tggaattgcc ggatgtttac ccgtgtccgg ggtacgacct cacgttgcag
     7621 cgtacgagaa tggactattg ctcagtgtcg tttgatgtcg gctgaggata ttcgccttgc
     7681 tcttgtaaca gagttagaag aatggaaaac ccctcacgat gtgatgattg atgagtgtcg
     7741 atcccggaat gattgatgtt gttaaattcc aacatcgtgc tcctggcgat agcctcaacg
     7801 gtattccaga atactaatac atctgttttc tgctctgttt tatcagcaac aggaagtttg
     7861 atattttgtt gaaatttatt aatgcaacca ttttgaatat gttcctcgtg cttttcattg
     7921 tcataagcat acagcccgac aaaaacatct cgcacgttac gggatatatt ttccagattc
     7981 gcctttggtt cctttgttaa atcaagcacc gataaatggg ctttaagcgt atccgcagct
     8041 attttctgaa tatcctctgg taaatcttta aattgcatag tataccctcc aagaactgtg
     8101 tattttctcg tgaaatatga gcgatatgtc gtgacgtccc tgtcacagat ttcatcacac
     8161 tgagaacgtg ccgatgaagg ttaagatatc ttcttcttcg aaacgctctt tcagcaggtc
     8221 acggaactct ttggcaattt cttcttccgc agcttcaaca cgcacgatac gtagcaccag
     8281 aacaggctgt tcgcttgtca gaatgcttaa tcgcagcgtg aattccctgt tgcccaggcc
     8341 ctcgtatggg atgcattcaa agcggaaagt ggcaggcatg atttctttgc ttttagcctc
     8401 cacggactcc atgactgagc ggctaccgct gaagttttgc tcttcatatt cagcactgcg
     8461 ttttgcatcg atggtaagac gacggacagc agagatagcc tgtttgatat ccagcacatt
     8521 accgtcgctg tcataggctg tgaggaagtc ggcccagtct tccagccatt cagcgagaga
     8581 cttctggagt tgtcttttgc cgtccacgtc acgtaatgcg cgatatggtg atgtggcttt
     8641 cagttccagc agtgccttgt tgtcagcgtg tccaggttgc tccagagtgc cgatattgaa
     8701 cacagtgact gctttcatcg tttccgcgtt aatgaagcag cgggagcact catcggcata
     8761 ccctttgctg tattcaacaa agtcatcaat actggttgtg gtcatgactc cacggaaacg
     8821 aaaacgttct gtcatgaatc gttccagact ttcgatattt acattgtccg gcaataacgc
     8881 aacagggcat tccgtaagcg gcaaattttc accagaaaaa gcagtagtgg tgagttttac
     8941 aatttcttta atggcattgc tatctaactg agacatgtat atttaccttt aatttatgta
     9001 tttagggata atgcgcgagc gttttaaggc ggggaatatt cagcgtaaaa cagcgtcatg
     9061 ttggccctgt ttatcaaaaa gctgtccctg gtctttctgg aacaaagtaa gctcaccacc
     9121 tttatttaca aacatcgggg tgttagttgt gtcttcttct gatttactac cacgcattgt
     9181 cgggcgtgtg aattttagtt tatgggaaac tgatacgcga ttttcatcaa gtgaagataa
     9241 ttcgaattca acacatacct ttcctttttt gtttgtgctg tttacgccaa aggcaacttc
     9301 acttaatacc gcgccaagtt tgttaacaaa taccccgccg tcgaggtcgt taatgaaaac
     9361 attaacatca gtttgatgct ggttcatatt aatatctccg tgtagaggtg tgttaaggct
     9421 tcagttgttc actgcattta aagcctggtt ttgtttgtga attaaaaact atcgctgccg
     9481 tactgccagt aacctggggc gtccgtgtga ttcgggttta cgtttttgct gcgattgtct
     9541 ttgtctttga gagtggaaac catatttgct accgtttctg gtttctttcc ttcagctttt
     9601 tctttcagtg tctgccctgt ttctgcaatg aatggaatgc ggatttccat ctggtaaccg
     9661 tctgcgccag tctttctgtt tgtggttaat actttctgga ggacgagtcc gattttcttt
     9721 ccgtgaaact caggtgcaac atatttactg acggaaacca tatgttgcgt taactgccca
     9781 attcctgtgc atcccatcat ggcgtgaata atgctcgccc caaatttatt ttcagtaccg
     9841 tcattcttct gaacacaaac actgagatat tggattttcc gtccgtcgtc ggattctcca
     9901 gaaaactcaa tgaatcgggc acctttttct gattgcttga gttctgcttc ggtgatgtta
     9961 atgatatgcg cacccgtttc ggtaataaaa ctaccttgtc cggcggtcag tgctgattct
    10021 tcgttataag taaaaatcac ttcatccatt agttattaac cccccattgt tctccaaata
    10081 caaaaccaat ttccgccagt gcttcgtcca ttttgtcgat gaactccggc accatctcat
    10141 caaaactcgc catgtacttt tcatcccgct cgaccacgac ataatgcagg ccttcacgct
    10201 tcatgcgtgg gtcgtagttg gcgaagtacc aggcgtcctt gtctgtaacc cacatgctga
    10261 attgcacctg ggccatgtaa gcaggcttga tagcgtcaaa gccgccaagc cggaatttca
    10321 tgaagtcgcg ggaagtaaaa gggcatttaa gctcaagacc gttaccgtcg ctgcataaac
    10381 cgtcgggaga gcaggcggtg cgcatgcttt cgtcacgaaa aattattggc gtttccgaga
    10441 cggtaacatc cgcgataaat tcgaagaggg cacgggcatc atcttcgtat tgctttcccc
    10501 atgcgagcga cttagcgtta acttccggtg ccacgccagt acaaacttca gccagcaggg
    10561 tgtggaagta tgacattttt gtatcaggcc atttgctgcc ggaacgtggc tttgctatca
    10621 cattgtgaac ttctgaagca gtgataacac cgagtctcag cttgtgccat ccatcatcgc
    10681 cctggtcgag gctggtaata tccacgccag ttcgctggag aataatttct ggtgtcatga
    10741 cacggcctcg ctgttatttt ccgtggtggc gtgaattttc gcttccgccg tggccttgtt
    10801 tctggcagct ttctttttga caaaatcaag cgttttgaca gcttcttcct gactgagata
    10861 ttcatgtgat gcaatcgtgc gacggaatgt tttggaacat aagggaagca ggtcatccca
    10921 tgtcttgtta atttcggtga ttgcctgagt gatctcattg ataatctcat ctgatgcggg
    10981 agtgacgtca cgctcaggga tgtgatcagc attcaggatg ataccttcac cgccctgagt
    11041 gttcaggtag tcgatagctg tatccaggcg atcgcgacgg ggccagtatt tgctggcgcg
    11101 cttaacaatg gcttttctgg ccatttcatc ggggaaacta tcccacgggc tacttcctcc
    11161 gttatttccg gctttgctgc acgccctgat gacctcgatt tccctgttgc tcatctcttc
    11221 ggtcagatag tcgccttcgg atgtttttac gacacaataa ccgccaatgc gtgcgcccct
    11281 gtctacgaat gggttgtatt tgtgcgtggg ggcgcagtca ataccgttgg actcgtaaat
    11341 gtccttctcg taaacaagtt tgcattgccc ccactgaatg gctcctgtga cctgtgccag
    11401 atgcagaagc cccatataac tgatatccag gcatacagcc ttttttcgtg ggaccagata
    11461 cgccagcttg ctggccgggt tcagggttat gccgatggcg gcaacattga tgatagcgtt
    11521 ctgtgcgctg ggcagatttg cccgtgctgt gtcagccaga aaggcatttt tctggaattg
    11581 ctgaattgca aactgacttt ccttcgccca tgtcagcgtc ggttcagtta atgcctcgca
    11641 gaaaaagcgc tcctgctgct taacaaattc aacgatatcg aacatttttt ggtcctgaaa
    11701 atcagaaagg acagggggag aattttctct cccattcttc ttccgcccga gcataggcga
    11761 tcgctgagat ataatcgttg tacgcctctt cagctttttc gccagtgagt gccagttggg
    11821 cttctttggg taaaaaaagg ctgctcataa gcaatggttt atcggggaac atgctgataa
    11881 gctcctgcgc ccgatcatca atccatttat ccttttcatc ctgaatttgc tgattaatcc
    11941 agcgacgctc ctctatgcgg tcgcaggtga ggtatgcgtt catggcggaa ctcctgattc
    12001 cggttaatgc attaaattaa tttgtcggga aagctgacat acagggcagt tacattcttc
    12061 ctcctgctcc ttagcgaaga aatatgcagc ggcctgtaat gcgatgtctt ctggatgttc
    12121 cgcgataaac ataacattgc cttccgtatc aataacagaa atagcctcat cagacaggat
    12181 gacaaaatag gcgatgattt tatcatccat aaaaacttct cccattatcg ttcctgctgg
    12241 agttacgacg ctttttacat tgatatttat tttttgattg agcatgatat ttcctttcag
    12301 gctggtgaga ttaacggttg gcctttattg ttcaggtaaa cttctattgc atctgagata
    12361 atgcgaattt tttcaatcag tgaatgtgcg taaagtgcat tattaacgtt cgctgacgcc
    12421 atgtaataac gcccgttgta aagaattgct gtgccgggtt taacgtcctc gcgagaaact
    12481 aatgcggttc cgtagtgagg tttgagcatg acaaatcctc cggttaatta atccagatat
    12541 tttatttaat ccccgatatg tgatcgggga tggggttaat taaagattaa cgttgaaacc
    12601 aaagcgggaa gatttttccg atgtgcggga aatatccagc aatttacggc gcatttcttc
    12661 cgtcagatgc ttgaaggcgt taaattcggc gacagcggcg tcaacgttat agcctttgct
    12721 gtgtagctca ttgaggatgc gcatagttgg gcttggcata tcgaagagca cggaagcatc
    12781 aaggctgata accttgcgat caacatagtt catcgtggcg taggggtgat gctctgaaaa
    12841 ccacgacaaa gggaaattga tattcatcgc aggagaagac agggccagtt gtttctgttc
    12901 ccatagttgt ttttccatgc gatcgaattc agcaatgtag gcttccttga atgcagctgc
    12961 ttttttgccg gtgaagccca tcaccagaaa aacgaagccg tttttggtga tttggtacat
    13021 tgggcgttgt tcgcctttgg cgtcggtgta ggtaacgtcc gcaaaattgc gggcgttaaa
    13081 ttccgtagaa caatccatca atcggatttt ttttaaaacg tcgtcgtgcc gcttgtcaaa
    13141 aaattcagca atagcaatag acgttgtgac aacgcgacca ttggtaatgg tgatttcagg
    13201 ttgagaaagg gcagggatag tagccataat ggcagcctcc ttgattggtg attgagaacc
    13261 accgcaggag gtcctaagct cgctggcggt ggactgtaca gggttaggac tactggcaat
    13321 caagggaacc agcccgaccg aagtcggccc catacagccc accattgata agatgtgcgt
    13381 gtatgtcgat acaaaaaaag acgctggcgc gtctgtatcg cctcgattgt cagcggggtc
    13441 ctaatcccga cacccgtttt ataaggtgtt cgccaaatat agccccgaca tcacacgcag
    13501 tcaataccgt cctttctcag aaacgctttg gcggtgccgc ctgcacccca aaacattccc
    13561 tgtatttggt cagcgccaac ttcctgccag tgttgcccgt tctcacgccg ttctcgctct
    13621 cgcgcgggga tactctctca tcgaccggat cgcacccgat gatacagcac gtttacgtgt
    13681 aggggtctaa acaggtcatt gacgctgtaa agctccagat tgttaaagag cattttgcgg
    13741 cgggttaagt cgcgccgtac gactgattta tgtagccctg tgtaagggcg cgatgtttct
    13801 ggcttgaaac aaatataact tgcggtgatt ttcatgtaaa taccgctggt gctttttggg
    13861 gcgaaaaatt attacttaac tgatttttaa agtgatttat tttttaaggg gacagataac
    13921 aggaggggat atgcaaagaa aaacccggcg catgggccgg gctatttatc tggtcctgta
    13981 ctcattcagt agagtagggg ccaagaaatc gctctccgtt gaagtgaata agatgagaag
    14041 gtgcatcagc cacccatacc tctgtttccc atgcgatttc accaagatat cgtcccatga
    14101 tggagcgatt tggaaaagcg gtcacataga caagtccggc tgttgatccg gcaaacagcc
    14161 tggcaagctc agcatgccgc ttcccatcaa ccggtccatg actggtgaca gactcaacca
    14221 gtagcagcca gtttttcgca gtaaaatgta gcaccacatc tggcatttta ccgtgtgaat
    14281 ccacatcaac acctagcccg gccagcagtg gagcgtcgaa gtagccccac ttttcgccag
    14341 tgtcgccagc atagaccagc acgctacccg gagcaaaacg tggggcgaag tcctctatta
    14401 tggcacggat aagttcgctg tgctcgccgg gactgagggt tatttgctgg cccgcagcaa
    14461 tttcaacagg gatacgattc tgttcgcgct ccttagcata gcgggtaacc agcgtttcac
    14521 gctcggctaa ataggttgca aggctatcgt gccatgccgg gctgccgaaa gtgcgtagca
    14581 tggacagagc agcaggttcg atctgataaa cagccttcgg gctgttcact gggcgatcgg
    14641 gcttgtccgg attgtagagg gctactccag cagcgcagaa ctgatgcata gactggcggc
    14701 ggaatgtctc acgagtattg ggtgcgtaga ccttgccgta gtgctcccga acccaattca
    14761 tgattggtgt aatgcccaca agcggatttt ccgcgtcggc ccatgctttt cccggtgtga
    14821 ggttcaggag tgccagcaaa cacaaagcag aacgctcatt ctgctgcgct ctgggcaggc
    14881 ctaaggatgc tataatttgc tgagcggcct caatataatc attttggttg ttcatgcagt
    14941 cagagctcct agcttagcgt caatatgttc ttgcgtgaga gtttcttgtt gcatagccca
    15001 cttgccaagc tctatcagag tgtcacggct tgggtactta atcatcttga ggtcggttgc
    15061 attgacctgt gtatgcccgt tgaagcgacg gaaatactta tccactgcgg tagtgttcag
    15121 gaacacggcc aagccacggg ccagcgcctc gggtaaccca tgtttgttct catgaaacac
    15181 gttcatgtga ttttcaaatc ccaacactga atactcactg aaggtagcag gatcaacgac
    15241 gcttgctacc actcggcgct tttcctcctt cgacgagaaa cgacgcacca cacaataaaa
    15301 accgttcggg taaagccact tttctgtctc gtcgttgcgc atgattgcgt tgggtttctt
    15361 caaaccttcc acaggccaga cagtgctact catactcaaa tggctcgggt agatcagggg
    15421 aacagttcct tcttctggca tgctgcgcaa atgagctttc agtcggaaat caactatcgg
    15481 tccggtcgat accttcacgc caatatcagc cagcgaacat tgtacggcgg atgataactc
    15541 aatggcgctt ttctcgtgcg tggtcggcac atgaataaac cgctccgaat cgtccgggta
    15601 cacaatttgg tcaaatgggt gttcgtaggt gaccaggtca aaaaagctat catcggtcga
    15661 agttgtgact gtaacagcct cctgaaggcc tccacgctcc aggcggatga taatattttc
    15721 ctgcaacaca tcgtcatccc tgaaagcttt gctacgtgat tcgaacaaat gaatatggcg
    15781 gattgctgcc cgctcaagta tgaaattacg gaacggacgg taatacggtc cgttgcagaa
    15841 actacgtggg attatggcca ctatctgccc gccaggtgca acctcaccta cagccagtgc
    15901 aacaaaggcg gaatacagat ttaccgtctc gataccgaca gtgcgaagag cctgccggtg
    15961 ggctgactgg ctattgatct tcttgtaagg cggattaagt attgcatggg tataacctcg
    16021 atcctgtaga ccttcggcgg ttgccagctc aatataatcc ccttcgatga tgcggggcgt
    16081 cactctcctg tacccggtta agtgcttcgc aaggtgacca cacagctttt tgtcgatttc
    16141 gtaggctgtt gcctcgacag actcaaagcc aaactcgcca accagccaac ggtcgaggaa
    16201 ggcgcaagac agtgcgccta caccagcgcc tgcgtccagt agacggcaag ttttcattgt
    16261 gctcggtgga aacaaggagg ccataaaacg ggccacgcca gaaggcgtca tgaactggcc
    16321 gaactcagct ttgtgtttac gcgcggtacg gggagacagc tcgcgacgca cgctgtctgc
    16381 gacgtctagc ttttgaagca taactaatcc tctttacgtg gcatgatgat gccaacgtgc
    16441 ttttgacatc ctccacattc tgcaggcgtg aaggatgtca aatcactctg tgagctcaat
    16501 caaaagtctc atcattccac tgcgatttga tgacctttcc gacaaactgg cagttttcgt
    16561 tacactcaat catgtcgaat cgtgggttga gcggctccag gtacgcttta ccgctctcac
    16621 gaatgaatcg tttgaaggta aattcatcgc cgttcattcg ggccacgcaa aaatcgccat
    16681 cctcgatttc ccgttcaggg tcaacgagta tcagcatccc ttcggggaaa ctgggtttac
    16741 caccctgcgg cgcggtcatt gaatgcccct tgacctccag ccagaacgac ctgtcactgg
    16801 cttttgtggt ggtggagatc catttaatcg catcttttgc ggtgtacgaa atatcatcca
    16861 ttgagaatgc gccagcttgt acgcaggaga gtaacggata ttcgtaagcg gggacacgag
    16921 ttaatggctc ttcaattgcg agcgccattt ctgcaataga ttttgccagg ctgggactga
    16981 aatctccgac ctttacattc agtatcttag caagctgtgc tgcgtggctg gcatttatgg
    17041 cgttgatgcc agccattagc tgattaacag cgctttgggt aacacctaat tcataggcta
    17101 atgattcctg cgacaggcct aactctttct ttttagcgtt aaaaatactt ttcagacgct
    17161 tggcgtcttc cagttgctca ggggttaacg gtttcttttt catgttcata ccatatcacc
    17221 tttggttata tgtaatgaaa tatctgcggt gttgacattt taataactgt gcgtaatgat
    17281 gtgggcattt tgaaggagtc agttatggcg atgaaaaaag agaccctggc tgattacgta
    17341 tacaggttcg gtcagaagaa agccgctaag gactttggtg tggcgcagag cgcgatcagt
    17401 aaagcgcttc tcgtcggacg tgaaatttac gtcaaaacct ttgatgacgg cagagttgag
    17461 gcggaagagg ttcgaccgtt ccctgcgttc gtaagaggcg atgattaatc ctggacacac
    17521 agcccatcgg aggtgtgagt gaacaggcaa atcaaaacgg ttatgcccga gcagtactgt
    17581 gatgctgatc gggactggat acaggagcaa ttgttacagc tagacccgac cacccgggta
    17641 aaaattgcag cagagtacgc gagagtgtac caggaagagt gggacaagga gcctgtatcg
    17701 ttcaggaagg gcaacagggc aagacggagc gccaacaccc ggttacgggt gtatgtccag
    17761 aagtacgcga gagccagccg tggctggatg ctttcgccag tagcggtacg gaagtttccc
    17821 ccgacaccat gatggcagtt agttgcgata gagataattt cacatgcaac taatttcctg
    17881 aaaggaagcg aggagggggt aagggggtgg agtcgttggt aaggcctgta tcccttggcc
    17941 taggccagaa ggcaggtaca taggttaggt agatcactgt ataagcgcgc aatagtttct
    18001 ggtgaaataa ttatcaagct aattattgag tgataaacta tgtcaacagc aacaaaatcg
    18061 acactgatcc gtacactgag cactgtacca ctgagaacag aggagcgtta cagctttctg
    18121 gctgatgttg taacgctcct tgagtcacag gggatgcatg tggcaagcaa tgtcacagta
    18181 agaatcgatg gcagaaattt ccgtgttgat attctcgcaa cagcaaaaac tggtgggagt
    18241 gtggctatcg agatcgatcg tagctctccg cgatcccgct cagtcatgaa attgcgtgaa
    18301 ttggcacgtc gtggcactga aggctttgtg ttgcttcgaa tgccaaagaa actgaccagt
    18361 tatagcgatg ctgggatcga tatcatcccg gcgaatggca agggggcgtc atgctgaaaa
    18421 tcaccccaaa ctttgctcag gagcgcggtt taaaccagct gcggcaccag tggaaacagc
    18481 atcgcacgta cctgatgtac gcacccactg gctctggcaa aactggtctt gcagcattcg
    18541 tcaccgccgg aatggttgag cgtggtatgc gagtgatgtt tgtctgtccg tacaccatcc
    18601 tgctgaacca gacagcggaa cgtttcaccg aatacggttt gccgtgggaa gaaatcagtt
    18661 ttgtatggcg tgatcaccct aactatgacc cgtcacgact gattcagatt gccagtgccg
    18721 acacgctgat ccgtcgtgag tttcctgaca acatcgatct gctgatcatc gatgaagcac
    18781 acatgaagcg tcgtgcgtta cttgaggtta tccgggacag agacatcagg gttcttggac
    18841 tttccggcac cccgttcgca gcctggatgg ggaagtacta cgaatgcctg atcaagccta
    18901 caacgattcg tgaacttatt cagcgtggtg atctgagcga ttacgagttt tttgcccctt
    18961 caatgcctga tctggctggc gtaaaaacca gtaacaccgt atttggacgg gattacaacg
    19021 aggagcagct cgcatccatc atggggagtt cggatctggt tggcgatatc gttcgcaact
    19081 ggcttgagaa tggcgaggat ttgccgacga tctgcttctg cgtgaatgtt gcgcatgcga
    19141 attttgttac ccgtgagttt ttacaggccg gtattggcgc agaggtgatg acggcagata
    19201 ccctccatga tgaacgacag gacatcattc gccgttttga agagggcgcg accaaaataa
    19261 ttgtcaacgt tggtgtactg gttgcgggat ttgacagcga tgttcgctgc ctgatctacg
    19321 cccgtccgac aaaatcagaa atccgttggt tgcagtgcat aggtagagcg ttacgtacag
    19381 ccccaggtaa aaaacgtgcg ttgattttcg accattctgg cacggttcat cggctgggat
    19441 tccctgagga tatcgagtat gacgaacttc cgggaaaaaa tgacggaatg aaagcgtctg
    19501 ctggcgggag tgaggttaag gctgagaaac ttcccagaga atgctccaaa tgccacttca
    19561 tgaagcctgc tggtgtacac atgtgtccca aatgtgggtt tcgtcctctt ggtggcgatg
    19621 atgtggcaac agaccgcgat cgaaaactct cacgcgtaaa taaagggaaa cgcgaataca
    19681 gccgcgagga aaaacagcgg tggtggagtg agatcaaggg gtatcagaat taccgcaacg
    19741 cgacaggtaa gccactgagt gacggatggt gcgctcatac ctacaaagag aagttcgggg
    19801 tgtggcctaa aggcttcagt aacgcgccgc tgcaaacctc ggttgaagtg cacaatttca
    19861 tcaggtcaaa gatcattgcc tatgccaaag ggcgcaagaa agccatgacc ggaggccagc
    19921 atgcagacta agaaggcggc tgtcgggcaa tgggggcgaa tttttgaata ttacggcatg
    19981 cctcctgtta ctggtgtgaa acattacaac ggcccatgcc cgatatgtgg tgccagaggt
    20041 aaatttcgct gtgatgataa agatggctcc ggttcgtgga tttgcgtctg tggtcacggg
    20101 gacgggatga agttgttgca acttgccacg ggtaagccct gggtgacgtt gtgtgatgaa
    20161 atcgatcggc tgatcggaaa tacctggaag agggagaaag tcagccagcc tgtaacagag
    20221 ataagcagaa agcgggagct ggtcatggat aagtttgccg gactcccgtg tctgcggggc
    20281 acaacaggtg aggcgtacct gcaggggagg ggaatactcc agttaccgac cgaatccgtg
    20341 cgtttttgtg accgtcagat cgccagcggg cgcgaatatc aggcaattta cgccattgca
    20401 acagatgaca gaggttctct ttgctatctg catcgtacgt tgctggatgg tgatcgcaag
    20461 gcgaatgtag aggcggctaa aaaaatgacc gcgctacagg agttgcctgg tttgcagcat
    20521 gccaaatcgg tggcaatacg cctgtatccg gtgtcgtcca ctctggggat agccgagggt
    20581 atcgaaactg cgctttcatg ccgtcaaatc ttccgctgca atgtgtggtc aacaatgaac
    20641 tccggtttta tggagaagtt tattgcgcca ccaggcgtta atcacctgat tatctttgca
    20701 gacaatgatg agcacggcgc aggtctggcg gctgccttta aatgtgggca taagaatctc
    20761 atgagtcgta atgacgttga gaaggtcagc attcgctggc ctgacttgcc ggattttaac
    20821 gacatgctca ttcaggggtg tgaagcccgt gaacatgtgt tgacgcgcaa attcaaagcg
    20881 gaggctgcct gatggaaata gagatgctca aggcggctaa tggcgtattt gtaccggcgt
    20941 atgagcgcga tttaccccga ctggcaaaat ttaaaaacgg tgagctgtat acactggaag
    21001 caaaacttac ccgtaaccca tcttttcacc ggaagatgtt cgcttttctt aatttctgct
    21061 ttcagtactg gtgcgctgaa catgctggat acgaattctc tgatgaagcg acgcagtttg
    21121 atgagtttcg taaaaatctg acaattcttg ccgggttcta tgatgtggtc acaaccataa
    21181 gaggcgaggt gagatatcgg gcaaaaagcc tgagttacgc gaatatggat caggatgagt
    21241 ttgaacgttg ctacaacgca atgattaatg ccgcgttaaa acatgtgttc gggtgctcaa
    21301 acaatcctga actgaataac cgcctgctgt cgtttttctg aggtgatgat gaagcaacgt
    21361 aaaccaaaaa aatgcaaagt gtgcggctcc tcgtttgtgc cgttccgctc atatcagaaa
    21421 gtttgctgtg gtcagtgcgc actggaactg gtcagaaaag aaaaggcgat agcttcagca
    21481 aaagagcagg cagacaagct gaaagcgcgc aggagggact tacagccccg cagttactgg
    21541 attaagcagg cacaacaggc ggtgaatgct tatatcaggg agcgggaccg tcatttgccg
    21601 tgtgtttcat gcgggacgtt cgattcagcc cagtgggatg caggccatta ccgtacaaca
    21661 gctgcggcac ctcagctcag atttgatgaa cgcaatatcc ataagcaatg cgtggtgtgt
    21721 aaccagtaca aaagcggaaa tctcgttccg tatcgtgtcg aactgattaa ccgcatcggg
    21781 caggaagcag tagacgaaat cgaatcaaac catagtcgcc accgctggac tgtcgaagag
    21841 tgcaaggcga tcaaggcaga gtatcaacag aaacttaaag acctgcgaaa cagcagaagt
    21901 gaggccgcat gacgttcacc gtaaaaacca ttcctgacat gcttgttgag gcatatggaa
    21961 atcagaccga agtggcccga atactgaact gcaatcgtgc cacagtcaga aaatacattg
    22021 gcgataaaga agggaaaaga cacgctatcg tcaacggtgt tcttatggtt caccgcggat
    22081 ggggtaaaga tactgatgcg tgatatccgg caggttcttg agcgctgggg ggcatgggcg
    22141 gcaaataacc atgaggatgt tacatggtcg cccattgctg ccggatttaa gaggctgatc
    22201 cccgaaaaag taaaatcacg tccacagtgt tgtgacgatg acgcgatgat tatatgcggg
    22261 tgtatggctc gccttaacag gaacaacagc gatctgcatg acttgctggt tgattattac
    22321 gtgttggggg agacgttcat ggcgctggca cggaaacatg ggtgctctga cacctgtata
    22381 ggtaaacgcc ttcacaaagc ggaggggatt gttgaaggca tgctgatgat gctgggagtg
    22441 aggcttgaga tggatcggta tgttgagcgt gaattgccgg gagggagaac ctctgtattt
    22501 tatcagcgaa aaaatagttt acgatcgtaa aaatctgcat atcatgataa gagtggttac
    22561 attgccacgc tgcttaaccc gccgatgcgc gggttttttt gtacccagaa tcctgtgagc
    22621 tatacggaaa gtacacagaa aggaaggtgc gaccacaatt aataacaaaa tcttaaaaat
    22681 cgcacatggc actattagtt ttctaaatat tgtgtatttt ttgtattgca ggatgaccct
    22741 gtaatgaagt ttgcgtaaca gaattttgct ctacgagttt gccagcctcc cccagtggct
    22801 ggctttttta tgtccgtaac atcctgtgta tcaataaatg ttgttgtcta cgtacgtcaa
    22861 gtagtcgcat gagatctgac cagatatgtt aaggttgcag ctctctttga atataattat
    22921 cattttcatt acgttattgt tacgtttatc cggtgcgccg taaaacgccg tccttcaggg
    22981 ggtggaggat gtcaagaata tagttatcgt atggtgctca aggagtattg tgtaatatga
    23041 aaataattat ttttagagtg ctaacttttt tctttgttat cttttcagtt aatgtggttg
    23101 cgaaggaatt taccttagac ttctcgactg caaagacgta tgtagattcg ctgaatgtca
    23161 ttcgctctgc aataggtact ccattacaga ctatttcatc aggaggtacg tctttactga
    23221 tgattgatag tggcacaggg gataatttgt ttgcagttga tgtcagaggg atagatccag
    23281 aggaagggcg gtttaataat ctacggctta ttgttgaacg aaataattta tatgtgacag
    23341 gatttgttaa caggacaaat aatgtttttt atcgctttgc tgatttttca catgttacct
    23401 ttccaggtac aacagcggtt acattgtctg gtgacagtag ctataccacg ttacagcgtg
    23461 ttgcagggat cagtcgtacg gggatgcaga taaatcgcca ttcgttgact acttcttatc
    23521 tggatttaat gtcgcatagt ggaacctcac tgacgcagtc tgtggcaaga gcgatgttac
    23581 ggtttgttac tgtgacagct gaagctttac gttttcggca aatacagagg ggatttcgta
    23641 caacactgga tgatctcagt gggcgttctt atgtaatgac tgctgaagat gttgatctta
    23701 cattgaactg gggaaggttg agtagtgtcc tgcctgacta tcatggacaa gactctgttc
    23761 gtgtaggaag aatttctttt ggaagcatta atgcaattct gggaagcgtg gcattaatac
    23821 tgaattgtca tcatcatgca tcgcgagttg ccagaatggc atctgatgag tttccttcta
    23881 tgtgtccggc agatggaaga gtccgtggga ttacgcacaa taaaatattg tgggattcat
    23941 ccactctggg ggcaattctg atgcgcagaa ctattagcag ttgagggggt aaaatgaaaa
    24001 aaacattatt aatagctgca tcgctttcat ttttttcagc aagtgcgctg gcgacgcctg
    24061 attgtgtaac tggaaaggtg gagtatacaa aatataatga tgacgatacc tttacagtta
    24121 aagtgggtga taaagaatta tttaccaaca gatggaatct tcagtctctt cttctcagtg
    24181 cgcaaattac ggggatgact gtaaccatta aaactaatgc ctgtcataat ggagggggat
    24241 tcagcgaagt tatttttcgt tgactcagaa tagctcagtg aaaatagcag gcggagattc
    24301 ataaatgtta aatacatctc aattcagtca gttgttgcct gtctgataat agatgtgtta
    24361 gaaaatttct gcatggtgaa tccccctgtg cggaggggcg actggtgaac ggtatgatct
    24421 ctttgatgat cgtaagcgag aatacgcggg tttggtggca ccaggccgaa ctcaccggga
    24481 ggcacccggc accatgcagt attcagagat taggcatata tccaggctcc tcatcgcagg
    24541 agccttttta catgcaaaaa aaaacgctcc tgggaagagc ggctggcaag aagaaacaat
    24601 atgaacaatt aattaacgat gggaataata ccttagagta atcaccttgc gcaactgtaa
    24661 gggcatattt catctttgcg ggctgttttt ctgtgtggct tctgtgtttc cggagggcag
    24721 cctgtacctt ttctgactca gaacattatc ccggccggga ggattcatgg catttaaaca
    24781 ctacgatgtg gtcagggcgg catcgccgtc agaccttgct gaacgaataa ctcaaaaact
    24841 gaaggaaggg tggcagcctt atggtagtgc gctgatttcg acagctggtt atggtgcaga
    24901 gtttatccag ccagttgtga gtgaggggga gcttccctca cttgcagagt caggcaatca
    24961 cccgcatgtt tcagcgaagc ctgaagcagc gccggaatat tactatgtga ttgcgcttgc
    25021 cggtcagtcc aatagcatgt catacggtga aggtcttccg ttgccggaga catatgaccg
    25081 tccggacccg cgtattaagc agctggcgcg ccgcagtacg gtgacaccgg gcggtgcagc
    25141 ctgtaagtat aacgacatca ttccggcgga ccattgtctg catgatgtgc aggacatgag
    25201 ccgccttaac catccgaaag cggacctgtc aaaggggcag tacggtaccg tggggcaggg
    25261 gctgcatatc gccaaaaaac tgctgccgtt tataccggcg aatgcgggca ttctgctggt
    25321 tccgtgctgt cgtggtggct ctgcgttcac caccggagct gatggcacat acagtgacgc
    25381 gagtggtgcc tcggagaatt caacccgctg gggtgtggac aagccgctgt ataaggacct
    25441 tatcggtcga acaaaagcag cactgaagaa gaacccgaaa aatgtgctgt ttgccgtggt
    25501 gtggatgcag ggggaatttg attttggcgg tacgccggta aatcatgccg cacagtttgg
    25561 tgcgctggtt gataaattcc gtgcagacct ggcggatatg gcaggtcagt gcgttggtgg
    25621 ctctgctggc ggtgttccct ggatatgcgg ggacacgacg tatttctgga agcagaagaa
    25681 cgaatccacg taccagacgg tgtacggcag ctataaaaac aaaacggaaa agaatatcca
    25741 tttcgtaccg ttcatgacgg atgagaacgg ggtgaatgtg ccgacgaaca aaccggaaga
    25801 agacccggac attccgggta tcgggtatta cggttcgaaa tggcgtgaca gctcagccac
    25861 ctggacgtca caggacaggg cgagccattt tagcacctgg gctcgccggg ggattatttc
    25921 cgaccgtctg gcaacggcga ttctggtgca cgccgggaga accgctgaat tcattaccgg
    25981 aaaacagcct gatatggtga ggcccaccgt accttccggt gaaggtccgg agagagaggc
    26041 ggaagccccg gccagtaagc gtaccctgat gagtctgctg gcatccggcg aagacctggc
    26101 atcacagggc tggcgctatt atcacaaacc ggcgggcgga gacagtgtta acaaaaacat
    26161 tgctgaagcg gtggtcagtg atgcgggggc tacgggaggt aaggctctgc aactgaacaa
    26221 accggaaaac cacatctggt ttctggagca tgatgcagcc ggacaggggg cggagttact
    26281 gaagaagggc ggacgtgtga gcgtacggtt taagttgccg ggttcactgg tgccgaatca
    26341 gtttgccctg ggcatttact ggcagttgtc gtccctgccg gagggggtga tgctgtcagg
    26401 agaaggtaac gatatgctga tgtctttctt cctgcagacg gatacgacga acctgaacgc
    26461 gatgcatcac aggaagccga atgcgaagct ggatacattc ggggtctttg ataacggatg
    26521 gcacacgctg gcttttgagt ttgccggaaa caacagcatt caggtgacac cagtactgga
    26581 tgagaaacga ggagcggcgt tcacactggt gaagtcaccg gcatcggggg cggcggacaa
    26641 actgcaactg accgatatat caaaatcggc gacgtatacg ctgctgattg acagcattgc
    26701 ggtggaagtg aacagcacag acactgcggc atgataaaaa aagccgccag ttaccggaat
    26761 ggactggcgg tggtaatacc catggagaaa tataaaggaa cgatactttc gtgctctgtt
    26821 tttttgaatg aaaacagttc ctattgtcaa caataacggt aagaaattat gacttttatt
    26881 catcaggtga tgctgtactt ctgtacggca gtctgtgtgc tgtatctcct ttcgggtgga
    26941 taccgggcca tgcgtgactt ctggcgcaga cagattgaca aaagggccgc tgagaaaatc
    27001 agcgccagtc agtcagccgg aagcaaaccc gaagagccgc tcatttagcg gcaactttct
    27061 taatcacacc tttcgacgag aaaatcccat gtcagaaatt acatccctgg tcactgctga
    27121 agcagtgaag gacgtcctgc gctctgaaga agtcctgagc gcactgaaac agaaacttcg
    27181 ccataacctg gaagcgcgtc ttgatgcaga agtggatgcc attctggatg aactgctggg
    27241 cgcaccggca gctccggagc cggaaggcat cgcgggtgag gggagtgctt cagatagcgg
    27301 tgaccccaca cctgacagcg acatgatgat gtaagcatgc gtcagggacc atcggtgtgt
    27361 gccggtggtc ttttttattg ttgtgagctt ccggcttgcg ggagacgggg tatgtaccag
    27421 atggaaaaaa tcacaacagg tgtgtcatac accacgtcag cggtgggaac gggctactgg
    27481 ttcctgcagt tgctggacag ggtttccccg tctcagtggg cggcaatagg cgtgctgggg
    27541 agtctgctgt ttgggctgct gacatacctg accaacctgt atttcaaaat cagagaagac
    27601 cgtcgtaagg cggcgcgggg agagtaagct gatgagcagg aaactccgct atggtttatc
    27661 ggctgtcgtt ctggcgctga ttgccgcagg ggcttctgcg cctgaaatcc tcgaccagtt
    27721 tctggatgaa aaggaaggta accacaccac agcataccgt gatggtgcgg gtatctggac
    27781 catctgccgt gg
//
LOCUS       DAZGHF010000060        27663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGHF010000060 DAZGHF010000000
VERSION     DAZGHF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.060"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            99..341
                     /gene="yjbE"
                     /locus_tag="ACHK3A_002618"
     CDS             99..341
                     /gene="yjbE"
                     /locus_tag="ACHK3A_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO8740677.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAASGGGDGSNTGTTTTTTTSTQ"
     gene            528..1166
                     /gene="yjbF"
                     /locus_tag="ACHK3A_002619"
     CDS             528..1166
                     /gene="yjbF"
                     /locus_tag="ACHK3A_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO8740678.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWIEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1163..1900
                     /gene="yjbG"
                     /locus_tag="ACHK3A_002620"
     CDS             1163..1900
                     /gene="yjbG"
                     /locus_tag="ACHK3A_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO8740679.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLARLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1900..3996
                     /gene="yjbH"
                     /locus_tag="ACHK3A_002621"
     CDS             1900..3996
                     /gene="yjbH"
                     /locus_tag="ACHK3A_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO8740680.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQTKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4043..4321)
                     /locus_tag="ACHK3A_002622"
     CDS             complement(4043..4321)
                     /locus_tag="ACHK3A_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740681.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4535..4945
                     /gene="psiE"
                     /locus_tag="ACHK3A_002623"
     CDS             4535..4945
                     /gene="psiE"
                     /locus_tag="ACHK3A_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO8740682.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5039..5929)
                     /gene="malG"
                     /locus_tag="ACHK3A_002624"
     CDS             complement(5039..5929)
                     /gene="malG"
                     /locus_tag="ACHK3A_002624"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO8740683.1"
                     /translation="MAMVQPKSQKTRLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRVTPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5944..7488)
                     /gene="malF"
                     /locus_tag="ACHK3A_002625"
     CDS             complement(5944..7488)
                     /gene="malF"
                     /locus_tag="ACHK3A_002625"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO8740684.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7642..8832)
                     /gene="malE"
                     /locus_tag="ACHK3A_002626"
     CDS             complement(7642..8832)
                     /gene="malE"
                     /locus_tag="ACHK3A_002626"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO8740685.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            9197..10312
                     /gene="malK"
                     /locus_tag="ACHK3A_002627"
     CDS             9197..10312
                     /gene="malK"
                     /locus_tag="ACHK3A_002627"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO8740686.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10384..11724
                     /gene="lamB"
                     /locus_tag="ACHK3A_002628"
     CDS             10384..11724
                     /gene="lamB"
                     /locus_tag="ACHK3A_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO8740687.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11962..12882
                     /gene="malM"
                     /locus_tag="ACHK3A_002629"
     CDS             11962..12882
                     /gene="malM"
                     /locus_tag="ACHK3A_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO8740688.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGIRGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            13061..13558
                     /gene="ubiC"
                     /locus_tag="ACHK3A_002630"
     CDS             13061..13558
                     /gene="ubiC"
                     /locus_tag="ACHK3A_002630"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO8740689.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13571..14443
                     /gene="ubiA"
                     /locus_tag="ACHK3A_002631"
     CDS             13571..14443
                     /gene="ubiA"
                     /locus_tag="ACHK3A_002631"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO8740690.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14598..17021)
                     /gene="plsB"
                     /locus_tag="ACHK3A_002632"
     CDS             complement(14598..17021)
                     /gene="plsB"
                     /locus_tag="ACHK3A_002632"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO8740691.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPGVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            17192..17560
                     /gene="dgkA"
                     /locus_tag="ACHK3A_002633"
     CDS             17192..17560
                     /gene="dgkA"
                     /locus_tag="ACHK3A_002633"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO8740692.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17670..18278
                     /gene="lexA"
                     /locus_tag="ACHK3A_002634"
     CDS             17670..18278
                     /gene="lexA"
                     /locus_tag="ACHK3A_002634"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO8740693.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18351..19676
                     /gene="dinF"
                     /locus_tag="ACHK3A_002635"
     CDS             18351..19676
                     /gene="dinF"
                     /locus_tag="ACHK3A_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO8740694.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            19792..20001
                     /gene="yjbJ"
                     /locus_tag="ACHK3A_002636"
     CDS             19792..20001
                     /gene="yjbJ"
                     /locus_tag="ACHK3A_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO8740695.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(20043..20558)
                     /gene="zur"
                     /locus_tag="ACHK3A_002637"
     CDS             complement(20043..20558)
                     /gene="zur"
                     /locus_tag="ACHK3A_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO8740696.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20714..21709
                     /gene="dusA"
                     /locus_tag="ACHK3A_002638"
     CDS             20714..21709
                     /gene="dusA"
                     /locus_tag="ACHK3A_002638"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO8740697.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDVDPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            21843..22085
                     /gene="pspG"
                     /locus_tag="ACHK3A_002639"
     CDS             21843..22085
                     /gene="pspG"
                     /locus_tag="ACHK3A_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO8740698.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(22251..23234)
                     /gene="qorA"
                     /locus_tag="ACHK3A_002640"
     CDS             complement(22251..23234)
                     /gene="qorA"
                     /locus_tag="ACHK3A_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO8740699.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPNEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            23317..24732
                     /gene="dnaB"
                     /locus_tag="ACHK3A_002641"
     CDS             23317..24732
                     /gene="dnaB"
                     /locus_tag="ACHK3A_002641"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO8740700.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            24785..25864
                     /gene="alr"
                     /locus_tag="ACHK3A_002642"
     CDS             24785..25864
                     /gene="alr"
                     /locus_tag="ACHK3A_002642"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO8740701.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            26117..27310
                     /gene="tyrB"
                     /locus_tag="ACHK3A_002643"
     CDS             26117..27310
                     /gene="tyrB"
                     /locus_tag="ACHK3A_002643"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO8740702.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         6460 a         7016 c         7477 g         6710 t
ORIGIN      
        1 tccccgcgcc gtaattcgca tgctttagtt gtgtatactc gatcccgccc gaaatgtttt
       61 tgggtaaatc tccattcatt caatgaaggg aaattgttat gaaaaaagtt ctgtatggca
      121 tttttgccat atctgcgctt gcggcgactt ctgcgtgggc tgcacctgta caggtgggcg
      181 aagcggcagg gtcggcagca acgtcggttt cggcggggag ttcctccgcg accagcgtca
      241 gcaccgtaag ctcggcggtg ggtgtcgcgc tcgcggcaag cggtggcggt gatggttcta
      301 ataccgggac cacaacaacc acgaccacca gtacccagta ataaagtatg tatccccaaa
      361 ataattcgag ttgcaggaag gcgacaagcg agtgagtccc caggagcata gataactatg
      421 tgaccggggt gaacgagtgc ggtcaccgca tctgcagctt gaagtatgaa ggggattaac
      481 cataaccaca ctccggtgtg gttattctgc ccctctggag aagagtcgtg aagcgacctg
      541 cactcattct tatctgcctg ctattacagg cctgttcagc cacgactaaa gagctgggca
      601 attcactgtg ggacagtctg ttcggcacgc caggcgtaca gctgacggac gatgatattc
      661 aaaatatgcc ctacgccagc cagtacatgc agcttaatgg cgggccgcag ttatttgtgg
      721 tgctggcctt cgctgaagac ggacaacaaa aatgggtcac tcaggatcag gccactctcg
      781 tcacacaaca tggtcgtctg gtgaagactt tgcttggcgg cgacaacctg attgaagtga
      841 ataaccttgc cgccgacccg ctgattaaac ccgcacaaat tgttgatggt gcaacatgga
      901 cgcgcacgat gggctggatc gagtaccagc aggtacgcta cgccaccgca cgctcagtct
      961 tcaaatggga tggcaccgat accgtcaagg tcggcagcga tgaaaccccg gttcgcgtgc
     1021 tggacgaaga agtctccacc gaccaggcgc gctggcataa ccgctactgg atcgacagcg
     1081 aagggcaaat tcgccagtcg gaacagtatc tcggcgcgga ttatttcccg gtgaaaacca
     1141 ctctcatcaa ggcggcaaaa caatgattaa acaaactatt gtcgcattga ttttgagtgt
     1201 gggggcgtca tcggtcttcg cggcaggaac cgtcaaggtg ttcagcaatg gcagcagtga
     1261 ggccaaaacg ctaacgggcg cagagcattt aatcgatctg gtagggcaac ctcgactggc
     1321 aaacagctgg tggcctggtg cagtgatcag cgaagagctg gcaacggcgg cagcattgcg
     1381 gcagcagcag gcgctactgg cacggctggc agaactggcc gcagattcca gcgctgacga
     1441 tgccgctgcg attaacgcct tacgccagca aattcaggcg ttgaaagtga cgggcaggca
     1501 aaaaatcaat cttgatcccg atatcgtgcg cgttgccgaa cgtggtaacc caccgttgca
     1561 gggcaactac acactgtggg tcggaccacc accgtccact gtcacgttgt tcgggcttat
     1621 cagccgtcct ggcaagcagc cattcactcc aggtcgcgac gtggcgagct atctctccgg
     1681 gcagaacctg cttagcggtg cggatcgcag ctacgcgtgg gtggtttacc cggacggacg
     1741 cacgcaaaaa gcgccggtgg cttactggaa caagcgtcac gtagagccga tgcccggcag
     1801 cattatttat gttggcctcg cggactccgt ctggagtgag acacctgatg cccttaacgc
     1861 cgacattctt cagactctga cgcagcggat accccaataa tgaaaaaaag acatctgctt
     1921 agcttactgg cgctgggtat tagcacagct tgctacggcg aaacatatcc tgcgcccatt
     1981 ggtccgtcgc aatcggattt cggtggcgta ggattattac aaacgcccac cgcgcgcatg
     2041 gcgcgggaag gggagctgag tctgaactat cgcgataacg atcagtaccg ttattactca
     2101 gcttcagtgc aactcttccc gtggctggaa acaacgctgc gctacaccga cgtgcgcacc
     2161 cggcagtaca gcagcgtcga agcgttctct ggcgatcaaa cgtataaaga taaagccttc
     2221 gatctcaaac tgcgtttgtg ggaagagagt tactggctgc cgcaagtggc ggttggtgcg
     2281 cgggatatcg gcggtacggg gctgttcgat gcggaatatc ttgtcgccag caaagcctgg
     2341 gggccattcg attttacgct cggcctgggc tggggatatc tgggcaccag cggtaatgtg
     2401 aaaaatccgc tctgttcggc cagtgacaaa tattgctatc gcgataacag ctacaaacaa
     2461 gcgggatcta tcgacggcag ccagatgttc cacggtccgg cctcactgtt tggcggcgtg
     2521 gaataccaga cgccctggca accgctgcgc ctgaaactgg agtatgaagg caataattat
     2581 cagcaggatt ttgccggtaa gctggagcaa aaaagtaagt ttaacgtcgg ggcgatttat
     2641 cgcgttaccg attgggccga cgttaacctt agctatgaac gtggcaacac ttttatgttt
     2701 ggcgtcacgc tgcgcaccaa ctttaacgat ctgcgcccgt cctacaacga taacgcccgc
     2761 ccgcaatatc aaccgcaacc acaggatgcc attttgcagc attcggtggt ggcgaatcag
     2821 ttaacgctgt tgaaatacaa cgccggactg gccgatccgc agatccagac gaaaggcgat
     2881 acgctgtatg tcaccggcga gcaggtgaaa tatcgtgatt cgcgtgaagg gatcatccgt
     2941 gctaatcgga tcgtgatgaa cgatctgccg gatgggatca aaacgatccg cattacggaa
     3001 aatcgcctta acatgccgca ggtgacgacg gaaaccgatg tcgccagcct gaaaaatcat
     3061 ctcgccggag agccgttggg ccacgaaacg aagctggcgc aaaaacgcgt cgagccagtg
     3121 gttccgcagt ccaccgagca gggctggtat atcgacaaat cacgctttga tttccatatc
     3181 gatccggtgt tgaaccagtc ggtcggtggc ccggaaaact tttacatgta tcagctgggc
     3241 gtgatgggaa cggcggattt gtggctgacg gaccatctgc tgaccaccgg cagcctgttt
     3301 gctaaccttg ccaacaacta cgacaagttt aactacacca atccgccgca ggactcgcac
     3361 ttaccgcgcg ttcgtaccca tgtgcgcgag tatgtgcaga acgatgtcta tgtgaataac
     3421 ctgcaagcca actacttcca gcatctgggc aacggcttct acggtcaggt ctacggaggt
     3481 tatctcgaaa ccatgtttgg cggtgcgggg gcagaagtgt tgtatcgccc gctggacagc
     3541 aactgggcgt ttggtctgga tgccaactac gttaaacagc gcgactggcg tagtgcaaaa
     3601 gatatgatga aattcaccga ctacagcgtg aaaaccggac atctgaccgc atactggacg
     3661 ccatcttttg ctcaggatgt gttagtgaaa gcgagcgtcg ggcagtatct ggcaggggat
     3721 aaaggcggca cgctggagat cgccaaacgc tttgatagcg gcgtggtggt gggtggctat
     3781 gccacgatca ccaacgtttc gaaagaggag tacggcgaag gggactttac caaaggcgtg
     3841 tatgtctctg taccgctgga tctcttctcg tctggcccga cacgtagccg tgcggcaatt
     3901 ggctggacgc cgctgacgcg tgacggtggt cagcaacttg ggcgtaagtt ccagttgtat
     3961 gacatgacca gcgaccgtag cgtcaatttc cgctaagtca tggaaaaggt gccagttttc
     4021 gcactcactg gcaccctcat tcttaatggc ctgtcgagcc atcaggaacg cagttcaccc
     4081 ccggtttgcg atctggtgtg taaaggcgaa aacattctgt cttcctgtcc gtcgatatcg
     4141 gttgcaggtc tggttgcctg agcgactggg ccatcattgg actggagaca ttgctttgcg
     4201 gtaggtcggc tttatcagca gtaccgagcg gaccagcata agcaggaaga acagagactg
     4261 ataacatcaa agcagcaaaa taaggcttca tttttaccac ctttatcagg ttacgtttca
     4321 tttgttccag aggaacattg tcgatttttc gcgcattgct ggtggctggg aatcacctga
     4381 atgggtgatt tttttggctg tgtgttttgc cagatgagac attgatggga gatgcccact
     4441 taattgagct gaacaaaaca tacacaaaaa atatagatct ccgtcacatt tttgcgttat
     4501 acaggaagct cgccactgtg aaggaggtac tgctatgacg tcactctctc gtccgcgcgt
     4561 ggagtttata tccaccattt tgcagaccgt actcaatctt ggcctgttgt gcctcggcct
     4621 gattttggtt gtcttcctcg gcaaagaaac ggtgcatctg gctgatgtgc tgttcgcgcc
     4681 agaacaaacc agtaaatatg agctggtaga agggctggtg gtttactttc tctatttcga
     4741 atttatcgcg ctgattgtga agtactttca gtccggtttt cacttcccgc tacgttactt
     4801 tgtctatatt gggatcaccg ccattgtgcg cttgatcatc gtcgatcata aatcgccact
     4861 ggatgtgttg atctactcgg cagcgatcct gttactggtg atcaccctgt ggttgtgtaa
     4921 ttcgaaacgg ctgaagcggg agtaaaaaaa ggcgctccgt ggagcgccga ataacagtca
     4981 caagttggga taacgtaagt tgagggtgca gcggcataac attggcagaa caacatcttt
     5041 aacctttcac accacctgcc gtcaggccgt tgaccagcca gcgttgagcc agcaagaaga
     5101 cgatggtgat cggtaatgca gacatcacgg cagcggcggc aaagtcaccc cacaggtagt
     5161 tttgcgggtt gaggtattgc tgcatcccca cagccagggt gtagctgttt acgtcacgca
     5221 gtaatagcga cgcgaccgga acttcagtaa tggcagcgat aaacgacagg atgaacacaa
     5281 ccgccagaat cggtactgac agcggcaaca ggacaaggcg gaaggcctgc cacggtgtcg
     5341 caccatccag cgccgccgct tcttccagcg aactgtcgat ggtttcgaaa taacctttga
     5401 tggtccagac atgcagcgca atcccgccca gatacgcgaa aattacgccg ccgtgagtat
     5461 tcaggccaat gaatggaatg tactcaccca gacgatcaaa caacgcatac aacgcgacca
     5521 gtgaaagtac tgccgggaac atctggaaaa tcagcattcc tttcagcagc gtcgctttgc
     5581 ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt ggagagcgcc acaatgccaa
     5641 tcgcggaaat cccggcgacc tttaccgagt tccacagcca tagcagtacc ggaaacggcg
     5701 gcggtgtaac gcgaccatca gcctgttcca cgctaaaacc taatgccagt ttccagtgat
     5761 cccaggagat ttgctccggg atcaggctgc cggtcgcaaa gttcccctgg cgcagtgaga
     5821 tagcgacgac catcagtagc gggaacataa tcgccgcgat aaaaagtagc agtagcaggt
     5881 gagtaataaa caaacgtgtt ttttgcgatt tcggttggac cattgccatt tttgttatct
     5941 cccttaatca aacttcattc gcgtggcttt caggttcact atcgccagcg cacccaccag
     6001 caggaagatc agcgtggcaa ttgctgccgc cagaccgaag tcctgacccc cgccgccttc
     6061 aaaagcgatg cggtaggtgt agttaacaag caggtcggta taaccggctg gcgtggtcgt
     6121 gccaagacga tccgggccgc cgttggttaa cagttgaatc agcacgaagt tgttaaagtt
     6181 aaaggcgaag ctggcgatca tcagcggcgt cagcggttta atcagcagcg gcagcgtaat
     6241 cttaaagaag ttctggaacg gacctgcgcc atccattgct gaggcttcat acaaatcgtc
     6301 cggaatcgct ttcagcaagc ccatgcagag gatcatcatg tacggataac ccagccaggt
     6361 attgacgata atcagcatcg ttcgggcggt ggtcggatcg ctgaaccagg cgggcttcac
     6421 gccaaacagc gcgctcaaca tcatgttgat ctcaccgaag ctctggttaa acaacccttt
     6481 gaaaatcaag attgaaatga acgatggcac cgcgtagggc agaatcagca ggacgcgata
     6541 gaccgctttg ccgcgcaacg cttcccactg caccagacac gccagaacca tgccgaccgc
     6601 caccgttaaa aagacagtga tcagcgagaa caccacggtc cagacgaaaa tggcgaggaa
     6661 cggtttctga atgccttcgt cggtgaagac gcgggtaaag tttttccagc cagtggtcac
     6721 ggtgtaaccg gggcttagct tttcatcgcc ccagttgccg tcggcggtaa tggactggta
     6781 aaagccaatt tggttattcg gacgatattt cacgccgctc tgattattcg tcaacgtgcc
     6841 gtcaccgtcg agtgtgtaga gcggctgcgt gccagaaaac tggcgcaggg agctcatcat
     6901 cactttgttg ccatccggca gaatggcggt aatgtcactc agcgcctgac gattctgggt
     6961 aatcacgcgt agattcgcgc gttcgccttc gggctgggcg gtcgtttctt tcagttgcag
     7021 tttttgctcg ccgccaaatt taaaagcgtc ggaaaggtaa tttttgccgg tttcgccgtc
     7081 gctgagcgcc agttgccact catcgcccgc cgggtaaaga ccaaagttat aggttttgcc
     7141 tgcttgccag gagcgatcta acaacacttc ctgtgcacgt tcaaaagtca gctggttagt
     7201 gctgctgtag ttggtgaagg caatggcgat ggtgcagacc agagggaaga ggacgaataa
     7261 tcccattcca gccattcccg ggtaaacata gcgccaggcg taggctttac gattggcgaa
     7321 aatatacagc cccgctgaac tcaatatcag cgtggtaatg gcgaacaggt attccccttg
     7381 tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg agcagaccta acactgacca
     7441 tttcagcgcg tcgctttgcc accaatgttt ctttttaatg acatccatgg ggttcttcct
     7501 cattccagga cggataaggc tttcacgcct tatccgacaa caactgcctg atgcgacgct
     7561 gacgcgtctt atcaggccta catacgtttc aatttcgtag gccggataag gcgtttacgc
     7621 cgcatccggc atttcacagc attacttggt gatacgagtc tgcgcgtctt tcagggcttc
     7681 atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca gtacgcacgg cataccagaa
     7741 agcggacatc tgcgggatgt tcggcataat ttcacctttc tgagcgttat ccatggtggc
     7801 ggcaatacgt ggatctttcg caagttcatc ctgataggac ttcagcgcta ctgcacccag
     7861 cggtttgtct ttattgaccg cttccagacc ttcgtcagtc agcagatagt tttcgaggaa
     7921 ctctttcgcc agctctttgt tcggactggc ggcgttaata cctgcgctca gcacgccaac
     7981 gaacggttta gatggttgac ctttgaaggt cggcagtacc gttacaccat aattcacttt
     8041 actggtgtcg atgttggacc atgcccacgg gccgttgata gtcatcgctg tttcgccttt
     8101 attaaaggca gcttctgcga tggagtaatc ggtgtctgca ttcatgtgtt tgtttttaat
     8161 cagatcaacc aggaaggtta gacccgcttt cgcgccagcg ttatccacgc ctacgtcttt
     8221 aatgtcgtac ttgccgtttt catacttgaa cgcataaccc ccgtcagccg caattagcgg
     8281 ccaggtgaag tatggttctt gcaggttgaa catcagcgcg ctcttacctt tcgctttcag
     8341 ttctttatcc agcgccggga tctcttccca ggtttttggc gggttcggta gcagatcttt
     8401 gttataaatc agtgataacg cttcgacagc gatcgggtaa gcaatcagct tgccgttgta
     8461 acgtacggca tcccaggtaa acggatacag cttgtcctgg aacgctttgt ccggggtgat
     8521 ttcagccaac aggccagatt gagcgtagcc gccaaagcgg tcgtgcgccc agaagataat
     8581 gtcagggcca tcgccagttg ccgcaacctg agggaatttc tcttccagtt tatccggatg
     8641 ctcaacggtg actttaattc cggtatcttt ctcgaatttc ttaccgactt cagcgagacc
     8701 gttatagcct ttatcgccgt taatccagat taccagttta ccttcttcga ttttggcgag
     8761 agccgaggcg gaaaacatca tcgtcgttaa tgcggataat gcgaggatgc gtgcacctgt
     8821 ttttattttc ataatctatg gtccttgttg gtgaagtgct cgtgaaaaca cctaaacgga
     8881 ctctagtttc tttatacggc aacctctttc catcctcctt gcccctacgc cccaccgttg
     8941 ctttgtgtga tctctgttac agaattggcg gtaatgtgga gatgcgcaca taaaatcgcc
     9001 atgatttttg caagcaacat cacgaaattc cttacatgac ctcggtttag ttcacagaag
     9061 ctgcgttctc atcctcccgc ctcctccccc ataaaaaagc cagggggtgg aggatttaag
     9121 ccatctcctg atgacgcata gtcagcccat catgaatgtt gctgtcgatg acaggttgtt
     9181 acaaagggag aagggcatgg cgagcgtaca gctgcaaaat gtaacgaaag cctggggcga
     9241 ggtcgtggta tcgaaagata tcaatctcga tatccatgaa ggtgaattcg tggtgtttgt
     9301 cggaccgtct ggctgcggta aatcgacttt actgcgcatg attgccgggc ttgagacgat
     9361 caccagcggc gacctgttca tcggtgagaa acggatgaat gacactccgc ctgcagaacg
     9421 cggcgttggt atggtgtttc agtcttacgc gctctatccc cacctgtcag tagcagaaaa
     9481 catgtcattt ggcctgaaac tggctggcgc gaaaaaagaa gtgattaacc agcgagttaa
     9541 ccaggttgcg gaagtgctgc aactggcgca tttgctggat cgcaaaccga aagcgctctc
     9601 cggtggtcag cgtcagcgtg tggcgattgg ccgtacgctg gtagccgagc caagcgtatt
     9661 tttgctcgat gaaccgctct ccaacctcga tgctgcactg cgtgtgcaaa tgcgtatcga
     9721 aatctcccgt ctgcataaac gcctgggccg cacaatgatt tacgtcaccc acgatcaggt
     9781 cgaagcgatg acactagccg acaaaatcgt ggtgctggac gccggtcgcg tggcgcaggt
     9841 tgggaaaccg ctggagctgt accactatcc ggcagaccgt tttgtcgccg gatttatcgg
     9901 ttcgccaaag atgaacttcc tgccagtaaa agtgaccgcc actgcaatcg atcaagtgca
     9961 ggtggagctg ccgatgccaa atcgtcagca agtctggctg ccggttgaaa gccgtgatgt
    10021 ccaggttgga gccaatatgt cgctgggtat tcgcccggaa catctactgc cgagtgatat
    10081 cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag caactcggca acgaaaccca
    10141 aatccatatc cagatccctt ccattcgtca aaacctggtg taccgccaga acgacgtggt
    10201 gttggtagaa gaaggtgcca cattcgctat cggcctgccg ccagagcgtt gccatctgtt
    10261 ccgtgaggat ggcactgcat gtcgtcgact gcataaggag ccgggcgttt aagcacccca
    10321 caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga aaagcaatga ctcaggagat
    10381 agaatgatga ttactctgcg caaacttcct ctggcggttg ccgtcgcagc gggcgtaatg
    10441 tctgctcagg caatggctgt tgatttccac ggctatgcac gttccggtat tggctggaca
    10501 ggtagcggcg gtgaacaaca gtgtttccag actaccggtg ctcaaagtaa ataccgtctt
    10561 ggcaacgaat gtgaaactta tgctgaatta aaattgggtc aggaagtgtg gaaagagggc
    10621 gataagagct tctatttcga cactaacgtg gcctattccg tcgcacaaca gaatgactgg
    10681 gaagctaccg atccggcctt ccgtgaagca aacgtgcagg gtaaaaacct gatcgaatgg
    10741 ctgccaggtt ccaccatctg ggcaggtaag cgcttctacc aacgtcatga cgttcatatg
    10801 atcgacttct actactggga tatttctggt cctggtgccg gtctggaaaa catcgatgtt
    10861 ggcttcggta aactctctct ggcagcaacc cgttcctctg aagctggtgg ttcttcctct
    10921 ttcgccagca acaatattta tgactatacc aacgaaaccg cgaacgacgt tttcgacgtg
    10981 cgtttagcgc agatggaaat caacccgggc ggcacattag aactgggtgt cgactacggt
    11041 cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg catcgaaaga cggttggtta
    11101 ttcactgctg aacatactca gagtgtcctg aagggcttta acaagtttgt tgttcagtac
    11161 gctactgact cgatgacctc gcagggtaaa ggtctgtcgc agggttctgg cgtcgcgttt
    11221 gataacgaaa aatttgccta caatatcaac aacaacggtc acatgctgcg tatcctcgac
    11281 cacggtgcga tctccatggg cgacaactgg gacatgatgt acgtgggtat gtaccaggat
    11341 atcaactggg ataacgacaa cggcaccaag tggtggaccg tcggtattcg cccgatgtac
    11401 aagtggacgc caatcatgag caccgtgatg gaaatcggct acgacaacgt cgaatcccag
    11461 cgcaccggcg acaagaacaa tcagtacaaa attacccttg cacaacaatg gcaggctggc
    11521 gacagcatct ggtcacgccc ggctattcgt gtcttcgcaa cctacgccaa gtgggatgag
    11581 aaatggggtt atgactacac cggtaacgcc aataccaaca ctaacttcgg caaagccgtt
    11641 cctgctgatt tcaacggcgg cagcttcggc cgtggtgaca gcgacgagtg gaccttcggt
    11701 gcccagatgg aaatctggtg gtaatagcaa aacctgggcc ggataaggtg cttgcgccgc
    11761 atccggcaac caacgcctga tgcgacgctt gcgcgtctta tcaggcctac aactacaacc
    11821 gctgccgaaa gtaggccgga taaggcgttt acgccgcatc cggcataaaa acagctttaa
    11881 ggggcgaaag cccctctgat tatcgggttt agcgcgctat tgcctggcta ccgctgagct
    11941 ccagattttg aggtgaaaac aatgaaaatg aataaaagtc tcatcgctct ctgtttatca
    12001 gcagggttgc tggcaagcgc acctggaatc agccttgccg atgttaacta cgtaccgcaa
    12061 aacaccagcg acgcgccagc cattccatct gccgcgctgc aacaactcac ctggacaccg
    12121 gtcgatcaat ctaaaaccca gaccacccaa ctggcgactg gcggccaaca actgaacgtt
    12181 cccggcatca gaggtccggt tgctgcgtac agcgtcccgg caaacattgg cgaactgacc
    12241 ctaacgctga ccagcgaagt gaacaaacaa actagcgtat ttgcgccgaa cgtgctgatt
    12301 cttgatcaga acatgacccc atcagccttc ttccccagca gttatttcac ctaccaggaa
    12361 ccaggcgtga tgagtgcaga tcgtctggaa ggcgttatgc gcctgacacc ggcgttgggg
    12421 cagcaaaaac tttatgttct ggtctttacc acggaaaaag acctccagca gacgacccaa
    12481 ctgctcgacc cggctaaagc ctatgccaaa ggcgtcggta actcgatccc ggatattcct
    12541 gacccggttg ctcgtcatac caccgatggc ttactgaaac tgaaagtcaa aacgaactcc
    12601 agttctagcg tgttggtcgg gcctctattt ggttcttccg ctccagctcc ggttacggta
    12661 ggcaacacgg cggcaccagc tgtggctgca cccgctccgg caccggtgaa gaaaagcgag
    12721 ccgatgctca acgacacgga aagttatttt aataccgcga tcaaaaacgc tgtcgcgaaa
    12781 ggtgatgttg ataaggcgtt aaaactgctt gatgaagctg aacgcctggg gtcgacatct
    12841 gcccgttcca cctttatcag cagtgtaaaa ggcaaggggt aattacgccc cacagtgctg
    12901 attttgcaac aactggtgcg tctcctggcg cacctttttt tctcctcctg tactattgtt
    12961 gcgtttttgt tgcgcattag ttcacttaat ttgctttaca tctcccgtaa atatttttct
    13021 gcgatacaat gcctttacgt tatgtaacgg agagttcggc atgtcacacc ccgcgttaac
    13081 gcaactgcgt gcgctgcgct attttacaga gatccctgcc ctggagccgc aactgctcga
    13141 ctggctgttg ctggaagatt ccatgacaaa acgttttgaa cagcagggaa aaacggtgag
    13201 cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa atccccgaag aactgccact
    13261 gctgccgaaa gagtctcgtt actggttacg tgaaattttg ttatgtgccg atggtgaacc
    13321 gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg agctggcgtt
    13381 acaaaaatta ggtaaaacgc cgttaggacg ctatctgttc acatcatcga cattaacccg
    13441 ggactttatt gagataggcc gtgatgccgg actgtggggg cgacgttccc gcctgcgatt
    13501 aagcggtaaa ccgctgttgc taacagaact gtttttaccg gcgtcaccgt tgtactaaga
    13561 ggaaaaaaat atggagtgga gtctgacgca gaataagctg ctggcgtttc atcgcttaat
    13621 gcgtacggat aagccaattg gcgcgttact gctgctctgg ccaacattat gggcgttgtg
    13681 ggtggcgaca ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg cgggcgtctg
    13741 gctgatgcgc gctgctggtt gcgtggtgaa tgattatgct gaccgcaagt ttgatggtca
    13801 tgttaagcgc acggcgaacc gaccactccc cagcggcgcg gtaacggaga aagaggcgcg
    13861 cgcgctgttt gtagtgctgg tactgatttc atttttactg gtgctgacgc tgaatacgat
    13921 gactattctg ttgtcgattg ccgcgctggc gctggcgtgg gtatacccgt ttatgaagcg
    13981 gtatacccat ctaccgcaag tggtgctggg cgcggcgttt ggctggtcga ttccaatggc
    14041 ttttgctgct gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc tcgccaatat
    14101 tctctgggcg gtggcatacg acacgcagta tgcgatggtt gaccgtgatg atgatgtgaa
    14161 gattggcatt aaatccaccg caatcctgtt cggccaatac gataaattga ttattggtat
    14221 tttgcagatt ggcgtactgg cgctgatggc gattatcggt gagttaaatg gcttaggctg
    14281 gggatattac tggtcaattc tggtggctgg cgcgctgttt gtttatcaac aaaaactgat
    14341 tgccaaccgc gagcgtgaag cctgctttaa agcatttatg aataataact atgttggtct
    14401 ggtactgttt ttagggctgg caatgagtta ctggcatttc tgatgatgta aaaaagccgg
    14461 atgatcatcc ggcttccttc tgggttgcct gatgcgctgc gcttctcagg cctacacaac
    14521 atatcgcaat ttattgaatt tgcagatcat ggtaggccga ataaggcgtt tgcgccgcat
    14581 ccggcaattc tccctgatta cccttcgccc tgcgtcgcac tctcaatcgt caaacgcacg
    14641 tctgatgtaa tcaactccgc cagcaactga tacaccttca tcgtttctgc cggttcggcg
    14701 tcgccgctat cgctgatata cccttcatca cgcagtgtca gcaccagaga actgaacacc
    14761 gccttgtcga agaactccgg cgcgttgatg ccgtgcagca cggagagacg ttgcgcgacg
    14821 gtgcggctct ctttctccag cgtaccgcgg ttgatcgacg ggttggcgct caacagccag
    14881 aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg cggccagcag ctgtagcgtg
    14941 cgagaatgcg ccgggttgat atgcaactca tcatcttgca gggtaatcag cccctgacgt
    15001 tgcatctcat ttgccagtgc atcaataacg cccggcaact cgtcgcgatc ccagcgcagg
    15061 aacagctccg ctttcagcat tgggtaaagc acattgacgt gctccatcaa tacgtcgcgg
    15121 gagatgtggc gatgctgggt gacaattgcc gccatcagcg aaggcagcac caacatatgc
    15181 gcaatgttgt tgcgatagta ggtcatcagc accgcttgct cgcgcggcag aatgatgatg
    15241 tcgccgatcg tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg atcgataagc
    15301 tcgctggcgc tggctgaagg aacggtagag tccgtggagt aaggcacgtt gcgcatcaga
    15361 tccaggtagc agttgagttg ctcggttaac tgctcgcggg tgagtgagcg ctgacgtgac
    15421 gccagaagcg cagtacaaca caggttcatg gcgtttgccg cgcctgcgtt gttaatgcgt
    15481 accatcagat cggcagcaat attattgacc gtcggcgtta accatgccgg acgcaccgct
    15541 tcgatgggat cgatagattc acgccagtca ggtacatgct ggttaaggta ggtcatcaac
    15601 ggcattggtt caccgaagtt gacgtaaccc tgaccgagat tacgcagctt gcttaaaccg
    15661 cgcagcatct gcggcaggct ctctttctct ttcgtcgcac cgcgcagttc tttggcgtaa
    15721 gtacccactt ccatgacgtg ctcataaccg atatagatcg gaatcagcgt aatcggacgc
    15781 gtgccgccac gcagcatcgc ctgaatggtc atcgacagcg taccggtttt cggatccagc
    15841 aaacgccctg tacgggaacg accgccttcc acgaaatact cgacggaata accacggctg
    15901 aacagttcgc cgagatactc acggaaaacg gtggaataga gcttattgcc tttaaacgta
    15961 cggcgaataa agaacgcccc cagacggcgg aaaatcggcc cggcaggcca gaaattcagg
    16021 ttgatcccgg cggcgatatg cggcggcacc agcccctggt gatacagcac gtaagaaagc
    16081 agcaggtagt ccatgtggct gcggtggcaa ggcacataca ccagctcatg accgtcgtgg
    16141 gccagctgac gaacgcgctc ggcgttatgg acgttgatgc cctggtaaag gcggttccag
    16201 gtgaagccca gaatacggtc agtcaggcgg atcatctcgt aagagaaatt cgccgcaatc
    16261 tcttccatca gcgcaatcgc gttctgctgc gctttttcat gggagatttt tttgctgcgc
    16321 gcttcatctt ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt aaacagatcc
    16381 tgacgagcag gcagacgtgg gcctacggca gccagacgtt gacgggcaaa gtgcatacgc
    16441 gccacgcgcg ccagtttctg cgcgatagtt ttatccgtgc cgtgttcatc cgccatacgg
    16501 cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca cagtacagcg
    16561 aaaaatttct gtacgccgtt aagcatacgc agcggcgggt tcacttcgcc tttttcacgc
    16621 cccggcgcgc gaccaaacat caccgatact ggcaccatct gcacatccag atttgggttg
    16681 ctacggtgca aatcgagata gtcgtggaac agcttaatag actcttcttt cggcgtgtaa
    16741 taggtgaaca cacgcggccc gccgtgaatg aacacatagc gcggcagtag cgtgccgtcg
    16801 atttccagcg gctctaacgg gtcaggcagg tcatgtgcca gacactgggc gcgcaacgtc
    16861 agcaagtcag ctttcgagtt gtacggtaaa acgtacataa ttggacgaga ggtatccagc
    16921 cccagttccg gggcaggatc tgccggaata gacttgcttt ttaccaggat gcttaatggt
    16981 aaattcagta atttgtagta aattcgtggc cagccggaca taaacgatgt aaagcctctg
    17041 gttaataatg caaatgcgcg gcaaggatag cagaaagtca tgggaaattc tgtggtatcc
    17101 gctcatgttt cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg tacactgata
    17161 ttgacgctca taatgtaaaa aggttctttc aatggccaat aataccactg gattcacccg
    17221 aattatcaaa gctgctggct attcctggaa aggtttacgc gctgcatgga tcaacgaagc
    17281 ggcattccgt caggaaggcg tagcggtatt gttggcggtg gtcatcgcct gctggctgga
    17341 tgtggacgca attacccgcg tgctgcttat cagctccgtg atgctggtga tgattgtgga
    17401 aatcctcaat agcgccatcg aagcagtggt tgaccgaatt ggctctgaat accatgagct
    17461 ttccggacgc gctaaagata tgggatccgc cgcggttctg attgcgatta tcgtcgccgt
    17521 gattacctgg tgcatcctgt tatggtcgca ttttggataa cccttccaga tttcgataaa
    17581 tctctggttt attgtgcagt ttatggttcc aaaatcgcct tttgctgtat atactcacag
    17641 cataactgta tatacaccca gggggcggaa tgaaagcgtt aacggccagg caacaagagg
    17701 tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg cgtgcggaaa
    17761 tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg aaggcgctgg
    17821 cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt ctgttgcagg
    17881 aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg cttctggcgc
    17941 aacagcatat tgaaggtcat tatcaggtcg atccttcctt attcaagccg aatgctgatt
    18001 tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg cattatggat ggcgacttgc
    18061 tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca cgtattgatg
    18121 acgaggttac cgttaagcgc ctgaaaaaac agggcaataa agtcgaactg ttgccagaaa
    18181 atagcgagtt taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc attgaagggc
    18241 tggcggttgg agttattcgc aacggcgact ggctgtaaca tatctctgag accgcgatgc
    18301 cgcctggcgt cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc atggcattcc
    18361 tcacttcatc tgataaagca ctctggcatc tcgccttacc catgattttc tccaatatca
    18421 ccgttccgtt gctgggactg gtcgatacgg cggtaattgg tcatcttgat agtccggttt
    18481 atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg ctgttgctgt
    18541 ttttacgcat gagcaccacc gggctgactg cgcaggctta tggtgccaaa aatcctcagg
    18601 cattagcccg tgcgctggtg caaccgttgc tgttggcgtt gggggctggg gcgttaattg
    18661 cgctgctgcg tacgccgatt atcgatctgg cgctgcatat tgttggcggc agcgaagcgg
    18721 tgctagaaca ggcgcgacgc tttcttgaaa tccgctggtt aagtgcaccg gcgtcgctgg
    18781 cgaatctggt attacttggt tggttactcg gtgtgcaata tgcccgtgcg ccagtaattt
    18841 tgttagtggt cggcaatatc ctcaacattg tgctggatgt ctggctggtg atggggctgc
    18901 atatgaacgt gcagggcgcg gcgctggcga cggttattgc ggaatatgca acattgctga
    18961 ttggtctgct aatggtgcgt aaaatcctca aactacgcgg aatctccggc gaaatgctga
    19021 aaactgcctg gcgaggaaac ttccgtcact tgctggcgct taaccgcgat atcatgctgc
    19081 gctcgctgtt gttgcaactc tgtttcggcg cgatcaccgt acttggcgcg cgactgggga
    19141 gtgacattat cgctgttaac gcggttctga tgacgttact cacctttacc gcctatgcgc
    19201 tggatggttt tgcctacgcg gttgaagcgc attccggtca ggcgtacggt gcgcgcgatg
    19261 gtagccagtt actggatgtc tggcgggcag cgtgccgcca gtcggggatc gtagcgttac
    19321 tgttttcggt ggtttatttg ctggcagggg aacacatcat tgcgttgctg acgtcgttaa
    19381 cccagattca gcagctggct gaccgctatc ttatctggca ggtgattttg ccgttggtcg
    19441 gcgtctggtg ttatctgctc gacggcatgt ttataggtgc aacgcgcgcc gccgaaatgc
    19501 gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg ctgccgtggc
    19561 tggggaatca tggtttgtgg ctggcattaa ccgtctttct ggctttgcgc gggctttctc
    19621 tggcggctat ctggcggcgt cactggcgca acggtacctg gtttgccgca acgtgacggt
    19681 taaaaattct gaataaataa tcctaagcca aattgctgac tacacttaat ctcacgttca
    19741 gaagaaaagt gaacgtactc tcattcacaa cctaacgatg aggtcttgat tatgaataaa
    19801 gatgaagccg gcggtaactg gaaacagttt aaaggtaaag tgaaagagca atggggcaaa
    19861 ctgaccgatg atgatatgac gatcattgaa ggtaaacgtg atcaactggt cggtaaaatc
    19921 caggaacgtt atggttatca gaaagatcag gcagaaaaag aggttgatag ttgggaaaaa
    19981 cgccatgatt accgctggta attaatccct cctgcccgac atgtacaagg atgtacaccc
    20041 ttttagcgag gtttcttttt cacctgaata gagtggtcat gatggcactg ttcgggatga
    20101 cgacacgctt ctacttctac acatgctgaa cataatccat gtgcttcaat aacattatgc
    20161 cgcagggcaa aacccatttt tgccgccagc gtatgcatga tgtcttccac gccttcagca
    20221 cattcttctt tcaccgcgcc gcagcgatcg caaataaaca tggctgaagt atgggtgggc
    20281 tgatcgaaca gatgacagag cacataactg ttggtggatt ccaccttatg cacaaaacct
    20341 tgttcaagca gaaactccag cgcgcgataa accgttggcg gcttggcttg cggttcagct
    20401 tcgcgcagca aatcaagcag atcataagcg ctgatagcgc catcctgcag actcatcagg
    20461 cgcaatactt ccaggcgctg tggggtcagg cgcacattac gctgtgcgca gattttttca
    20521 gcctgcgcta ataactcctg cgttgtggtc ttttccattg ggcacctcga cagtggggtt
    20581 aagaatgtcc ttactttacc atagttctcg cttaaacgcc gagaacaggg tgtcatggct
    20641 gtcacattgc ctgatcgcat attcgttatg ctatagtagc gcccccaatt taccagatgc
    20701 ttaaaaagtc gccatgccag cagaatcaca cactgtttat cccgcttacc gtttctcgat
    20761 tgccccgatg cttgactgga cggacagaca ttgccgctat ttcttgcgtc tgctttcccg
    20821 caatacgttg ctgtataccg aaatggtgac tacaggggcg attattcacg gtaaaggtga
    20881 ttacctggcg tacagtgaag aagaacatcc ggtagcgttg caactcgggg gtagcgatcc
    20941 ggcggcgctg gcacagtgtg cgaagctggc agaagcgcgc ggatatgatg agatcaacct
    21001 gaatgtcggc tgcccgtctg accgggtgca gaacggcatg tttggtgcgt gtctgatggg
    21061 taatgcgcag ctggttgccg actgcgtgaa agcgatgcgc gatgtggtgt cgattccggt
    21121 gacggtgaaa acgcgtattg gcatcgatga tcaggacagc tatgaatttc tctgcgattt
    21181 catcaacacc gtttccggca aaggtgagtg tgagatgttc atcatccacg cacgtaaagc
    21241 ctggctttcg gggttaagcc cgaaagaaaa ccgtgaaatc ccgccgctcg attatccgcg
    21301 tgtgtatcaa ctgaagcgtg actttccgca tctgacgatg tcgattaacg gtggcatcaa
    21361 gtcgctggaa gaggctaaag cgcatttgca acatatggat ggcgtgatgg tcgggcgcga
    21421 agcgtatcag aatccaggca ttctggcggc ggtagaccga gagatctttg gttcttcgga
    21481 taccgatgtc gatccggtgg cggtggtgcg tgccatgtat ccgtacattg agcgtgaact
    21541 cagccagggg acgtatctcg gccatattac ccggcatatg ctgggcttgt tccagggtat
    21601 tcctggcgcg cggcagtggc ggcgttattt aagtgaaaat gcccataaag cgggtgctga
    21661 cattaacgtg ctggaacacg cgctcaaact ggtggcggat aagcgttaac ttttcaccaa
    21721 aaagtagtca aattcaccac gccctgcgca ccgtcgcggg gcgttttgct gttaaatcaa
    21781 tagattattt ttggcatgat tcttgtaatg cctgcaagag atttcatatt tgggagagca
    21841 tcatgctgga actacttttt gtgattggct tttttgtcat gctgatggtc accggcgttt
    21901 cgttgctggg cattatcgcc gcgctggttg tggcgacggc cattatgttc ctcggcggta
    21961 tgctggcatt gatgattaag ttgctgccgt ggttactcct ggcgattgcg gtggtgtggg
    22021 ttattaaggc gattaaagca ccaaaagtgc cgaaatatca gcgttatgac cgctggcgtt
    22081 actaagggat tgtgcggatg atcacaacct aaggttttat ccttagaaca aaataggaat
    22141 tgataatcaa atctgtcact attgcgcctc taacagattc atcgtgctgt accctacata
    22201 cagccgaact ataaaaagaa agggcttccc agatggaagc cctatttctt ttatggaatc
    22261 aacaggctgg aaccttgcgt cgcccggctt tccagaatct catgcgcacg ctgtgcatcc
    22321 ttaagcggat atttctgctg ctcggcgaca tcgaccttaa tcacaccgct ggcaatcaaa
    22381 gagaacagtt cattactggc ctcggttaat tcctcccgtg tggtgatata gccttgtagg
    22441 gaagggcgtg tcacatacaa cgagcctttc tgattgagaa tgcctaagtt cacaccggta
    22501 accgcacctg atgagttgcc aaagctcacc attaagccgc ggcgttgcag gcaatccagc
    22561 gaccgttccc aggtgtctct gcccacggaa tcgtacacca cgcgcacttt cttgccaccg
    22621 gtgatctctt ttaaccgctc gaccagattc tcttcacgat agttaataac ctgccatgcg
    22681 cccgctttta gcgcactctg cgctttttgc gcggttccta cggtgccgat aagtttcgcg
    22741 ccaagggctt ttgcccactg gcaggcaatc aagccaacgc cgccagccgc tgcgtggaac
    22801 aggaactgct cattgggttt aatttcatag gttttgcgca gcagataata aaccgttaag
    22861 cctttcagga aggatgccgc agcttgctca aaagaaattg ccgcaggcag aatcgccgct
    22921 ttatccgcat taatgttatg cacagagctg taagcgccta atgccgattg tgcatagact
    22981 acgcgatcgc ctgccttaat atgctttaca ccactgccga ctttactcac gatgcctgcc
    23041 gcttcggtgc ctaatccgct gggtagcgat ggcggcgggt aaaggccgct gcggatatac
    23101 gtgtcgataa aattgatgcc gatggcttta ttttcgacct ggatttcatt ctccgccgga
    23161 tcggcaggag tgaactctac ggcttgaagt acttccgggc caccgtgctt gtgaaattca
    23221 attcgtgttg ccatgtgtcc tccagaacgt atcgtcaggg tctgcttcat atggtaaagt
    23281 ttcgacccat tctttatctc ggtaactcca ttcactatgg caggaaataa acccttcaac
    23341 aaacagcagg ctgaaccccg cgaacgcgat ccacaagttg ccgggctgaa agtgcctccg
    23401 cactcgatcg aagcggagca gtcggtgttg ggcggtttaa tgctggataa cgaacgctgg
    23461 gatgatgtag ctgagcgtgt ggtggcagat gatttttaca cccgcccaca ccgtcatatc
    23521 tttactgaaa tggcgcgttt gcaggaaagc ggtagtccta tcgatctgat tacccttgcg
    23581 gaatcgctgg aacgccaggg gcaactcgat agcgtcggtg gttttgctta tctggcagag
    23641 ctgtcaaaaa atacgccaag tgcggcgaac atcagtgctt atgctgacat cgtgcgtgaa
    23701 cgtgccgttg ttcgcgagat gatttcggtt gcgaatgaga ttgccgaagc cggttttgat
    23761 ccgcaggggc gcaccagcga agatctgctg gacctggctg aatcccgcgt ctttaaaatt
    23821 gccgaaagtc gtgcaaacaa agacgaaggg ccgaagaaca tcgctgatgt gctcgacgcc
    23881 acggtggcgc gtattgagca gttgtttcag cagccacacg atggcgttac cggggtaaac
    23941 accggttatg acgatctcaa caaaaaaacc gctggcttgc agccgtcgga tttgatcatc
    24001 gtcgccgcgc gtccgtcgat gggtaaaaca acatttgcga tgaacctcgt cgaaaacgcg
    24061 gcgatgttgc aggataaacc agtacttatc ttctcgctgg agatgccatc agaacagatt
    24121 atgatgcgtt ctctggcgtc gctgtcgcgc gttgaccaga ctaaaatccg taccgggcag
    24181 ctcgatgatg aagactgggc gcgcatttcc ggcaccatgg gtattttgct cgaaaaacgc
    24241 aatatctata tcgatgactc ctccggcctg acgccaacgg aagtgcgttc ccgcgcgcgc
    24301 cgtattgccc gtgaacacgg cggcatcggg cttatcatga tcgactacct gcaactgatg
    24361 cgcgtaccgg cgctttccga taaccgtacg ctggaaattg cagaaatctc ccgctcgctg
    24421 aaagcactgg cgaaagaatt gaacgtgccg gtggtggcgc tgtcccagtt gaaccgttct
    24481 ctggaacaac gtgccgacaa acgcccggtc aactccgacc tgcgtgaatc tggctctatc
    24541 gagcaggatg cggacttgat catgtttatc tatcgtgatg aggtgtatca cgaaaacagt
    24601 gatttaaaag gcatcgcgga aattattatc ggtaaacaac gtaacggccc aatcgggacg
    24661 gtacgcctga cctttaacgg tcaatggtcg cgcttcgaca actatgcggg gccgcagtac
    24721 gacgacgaat aataattatt ttatgaatta ggtaattaaa gcaaacactt atcaaggaac
    24781 acaaatgcaa gcggcaactg ttgtgattaa ccgccgcgct ctgcgacaca acctgcaacg
    24841 tcttcgtgaa ctggctcctg ccagtaaaat ggttgcggtg gtgaaagcga acgcttatgg
    24901 tcacggtctt cttgagaccg cgcgaacgct ccccgatgct gacgcctttg gcgtagcccg
    24961 tctcgaagaa gctctgcgac tgcgtgcggg gggaatcacc aaacctgtac tgttactcga
    25021 aggctttttt gatgccagag atctgccgac gatttctgcg caacattttc ataccgccgt
    25081 gcataacgaa gaacagctgg ctgcgctgga agaggctagc ctggacgagc cggttaccgt
    25141 ctggatgaaa ctcgataccg gtatgcaccg tctgggcgta aggccggaac aggctgaggc
    25201 gttttatcat cgcctgaccc agtgcaaaaa cgttcgtcag ccggtgaata tcgtcagcca
    25261 ttttgcgcgc gcggatgaac caaaatgcgg cgcaaccgag aaacaactcg ctatctttaa
    25321 taccttttgt gaaggcaaac caggtcaacg ttccattgcc gcgtcgggtg gcattctgct
    25381 gtggccacag tcgcattttg actgggtgcg cccgggcatc attctttatg gcgtctcgcc
    25441 gctggaagat cgttccaccg gtgccgattt tggctgtcag ccggtaatgt cattgacctc
    25501 cagcctgatt gctgtgcgtg agcataaagc cggagagcct gtcggttatg gtggaacctg
    25561 ggtaagcgaa cgtgataccc gccttggcgt agtcgcgatg ggctatggcg atggttatcc
    25621 gcgcgccgcg ccgtccggta cgccagtgct ggtgaacggt cgcgaagtgc cgattgtcgg
    25681 gcgcgtcgcg atggatatga tctgcgtaga cttaggtcca caggcgcagg acaaagccgg
    25741 ggacccggtc attttatggg gcgaaggttt gcccgtagaa cgtatcgctg aaatgacaaa
    25801 agtgagcgct tacgaactta ttacgcgcct gacttcaagg gtcgcgatga aatacgtgga
    25861 ttaatcgttc tgtaatattt gattgtctgt gccggatgcg gcgtgaatgc cttatccggc
    25921 caataaaatc ttaccaattc aataagttga tattctttca tgctcttata aaggtcgtgc
    25981 atctggcgga tgtacgtttg tcatgagtct cactctgttg ctaattgcca ttcgcccctg
    26041 aacatcctct cgatcttcgc cttcttccgg tttattgtgt tttaaccacc tgcccgtaaa
    26101 cctggagaac catcgcgtgt ttcaaaaagt tgacgcctac gctggcgacc cgattcttac
    26161 gcttatggag cgttttaaag aagaccctcg cagcgacaaa gtgaatttaa gtatcggtct
    26221 gtactacaac gaagacggaa ttattccaca actgaaagcc gtagcggatg cggaagcgcg
    26281 cctgaatgcg cagcctcatg gtgcttcgct ttatttaccg atggaagggc ttaacagcta
    26341 tcgccatgcc attgcgccgc tgctgtttgg tgcggaccat ccggtactgc aacaacagcg
    26401 cgtagcaacc attcaaaccc ttggcggctc aggggcattg aaagtgggtg cagatttcct
    26461 gaaacgctac ttcccggaat caggcgtctg ggtcagcgat cctacctggg aaaaccacgt
    26521 agcaatattc gccggggctg gattcgaagt gagcacttac ccctggtatg acgaagcgac
    26581 taacggcgtg cgctttaatg acctgttggc gacgctgaaa acattacctg cccgcagtat
    26641 tgtgttgctg catccatgtt gccacaaccc aacgggtgcc gatctcacta atgaccagtg
    26701 ggattccgtg attgaaattc tcaaagcccg cgagcttatt ccattcctcg atattgccta
    26761 tcaaggattt ggtgccggta tggaagagga tgcctacgcc attcgcgcca ttgccagcgc
    26821 tggattaccc gctctggtga gcaattcgtt ctcgaaaatt ttctcccttt acggcgagcg
    26881 cgtcggcgga ctttctgttc tgtgtgaaga tgccgaagcc gcaggccgcg tactggggca
    26941 attgaaagca acagttcgcc gcaactactc cagcccgccg aattttggtg cgcaggtggt
    27001 ggctgcggtg ctgaatgacg aggcattgaa agccagctgg ctggcggaag tagaagagat
    27061 gcgtactcgc attctggcga tgcgtcagga actggtgaag gtattgagca cagagatacc
    27121 agaacgcaat ttcgattatc tgcttaatca gcgcggtatg ttcagttata ccggtttaag
    27181 tgccgctcag gttgaccgac tacgtgaaga atttggtgtc tatctcatcg ccagcggtcg
    27241 catgtgtgtc gccgggttaa atacggcaaa tgtacaacgt gtggcaaagg cgtttgctgc
    27301 ggtgatgtaa tgcaggaaag caggctggag ttatccagcc tgcagtgaaa ttaaactgtc
    27361 gtcgctttca ctcttgcttt atagatgatt tttttgatgc catcgttcta cgtgagaggt
    27421 aataaaacgt tgttagttct aacttgttaa gtttatccca atgttctgga gtacttttcc
    27481 ctgttttacc gtgtggaata ataatttttg cacatctaac cattattaaa tttaataata
    27541 agtatcgggt ttgttacgat agaatttttc tatatcctca agaatatgct ttgccttatt
    27601 gcctgtgact atctgagatt caattatagt atcgttaaat tctaaagtta aagagaactc
    27661 ttt
//
LOCUS       DAZGHF010000061        27571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGHF010000061 DAZGHF010000000
VERSION     DAZGHF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.061"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            67..510
                     /gene="cmtB"
                     /locus_tag="ACHK3A_002644"
     CDS             67..510
                     /gene="cmtB"
                     /locus_tag="ACHK3A_002644"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO8740703.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILNQLLTAASEKQLADIISRG"
     gene            538..1926
                     /gene="cmtA"
                     /locus_tag="ACHK3A_002645"
     CDS             538..1926
                     /gene="cmtA"
                     /locus_tag="ACHK3A_002645"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO8740704.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSVI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKN"
     gene            1941..3218
                     /gene="yggP"
                     /locus_tag="ACHK3A_002646"
     CDS             1941..3218
                     /gene="yggP"
                     /locus_tag="ACHK3A_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO8740705.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTDKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3215..3530
                     /locus_tag="ACHK3A_002647"
                     /pseudo
     CDS             3215..3530
                     /locus_tag="ACHK3A_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            3552..4061
                     /gene="fumE"
                     /locus_tag="ACHK3A_002648"
     CDS             3552..4061
                     /gene="fumE"
                     /locus_tag="ACHK3A_002648"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO8740706.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSRMVKTALTLAV
                     TTTLKELTP"
     gene            4058..4771
                     /gene="yggC"
                     /locus_tag="ACHK3A_002649"
     CDS             4058..4771
                     /gene="yggC"
                     /locus_tag="ACHK3A_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO8740707.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WQELASFCDFSIFIHAPAQILRERLISRKIAGGLSRQEAEAFYARTDGPNVERVLVNS
                     RQANLILEMTEEGRYHFKS"
     gene            5056..6075
                     /gene="epd"
                     /locus_tag="ACHK3A_002650"
     CDS             5056..6075
                     /gene="epd"
                     /locus_tag="ACHK3A_002650"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO8740708.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6125..7288
                     /gene="pgk"
                     /locus_tag="ACHK3A_002651"
     CDS             6125..7288
                     /gene="pgk"
                     /locus_tag="ACHK3A_002651"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO8740709.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            7502..8581
                     /gene="fbaA"
                     /locus_tag="ACHK3A_002652"
     CDS             7502..8581
                     /gene="fbaA"
                     /locus_tag="ACHK3A_002652"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO8740710.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            8772..9632
                     /gene="mscS"
                     /locus_tag="ACHK3A_002653"
     CDS             8772..9632
                     /gene="mscS"
                     /locus_tag="ACHK3A_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO8740711.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            9771..10406
                     /gene="argO"
                     /locus_tag="ACHK3A_002654"
     CDS             9771..10406
                     /gene="argO"
                     /locus_tag="ACHK3A_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO8740712.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIVHAQALLS"
     gene            10499..11239
                     /gene="yggE"
                     /locus_tag="ACHK3A_002655"
     CDS             10499..11239
                     /gene="yggE"
                     /locus_tag="ACHK3A_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO8740713.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            11407..12303
                     /gene="ygfI"
                     /locus_tag="ACHK3A_002656"
     CDS             11407..12303
                     /gene="ygfI"
                     /locus_tag="ACHK3A_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8740714.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(12300..13778)
                     /gene="scpC"
                     /locus_tag="ACHK3A_002657"
     CDS             complement(12300..13778)
                     /gene="scpC"
                     /locus_tag="ACHK3A_002657"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO8740715.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPNAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(13802..14587)
                     /gene="scpB"
                     /locus_tag="ACHK3A_002658"
     CDS             complement(13802..14587)
                     /gene="scpB"
                     /locus_tag="ACHK3A_002658"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO8740716.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(14598..15593)
                     /gene="meaB"
                     /locus_tag="ACHK3A_002659"
     CDS             complement(14598..15593)
                     /gene="meaB"
                     /locus_tag="ACHK3A_002659"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO8740717.1"
                     /translation="MINEAKLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNRLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(15586..17730)
                     /gene="scpA"
                     /locus_tag="ACHK3A_002660"
     CDS             complement(15586..17730)
                     /gene="scpA"
                     /locus_tag="ACHK3A_002660"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO8740718.1"
                     /translation="MSKVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSAAE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGRKDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(17934..18827)
                     /gene="argP"
                     /locus_tag="ACHK3A_002661"
     CDS             complement(17934..18827)
                     /gene="argP"
                     /locus_tag="ACHK3A_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO8740719.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18968..19153
                     /locus_tag="ACHK3A_002662"
     CDS             18968..19153
                     /locus_tag="ACHK3A_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740720.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFASWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            19209..19868
                     /gene="rpiA"
                     /locus_tag="ACHK3A_002663"
     CDS             19209..19868
                     /gene="rpiA"
                     /locus_tag="ACHK3A_002663"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO8740721.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            20124..21356
                     /gene="serA"
                     /locus_tag="ACHK3A_002664"
     CDS             20124..21356
                     /gene="serA"
                     /locus_tag="ACHK3A_002664"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO8740722.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLAEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            21585..21644
                     /gene="ibsC"
                     /locus_tag="ACHK3A_002665"
     CDS             21585..21644
                     /gene="ibsC"
                     /locus_tag="ACHK3A_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO8740723.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(21745..22347)
                     /gene="fau"
                     /locus_tag="ACHK3A_002666"
     CDS             complement(21745..22347)
                     /gene="fau"
                     /locus_tag="ACHK3A_002666"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO8740724.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTLPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(22368..22551)
                     /gene="ssrS"
                     /locus_tag="ACHK3A_002667"
     ncRNA           complement(22368..22551)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHK3A_002667"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22593..22922)
                     /gene="zapA"
                     /locus_tag="ACHK3A_002668"
     CDS             complement(22593..22922)
                     /gene="zapA"
                     /locus_tag="ACHK3A_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO8740725.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALFEQGRITE
                     KTNQNFE"
     gene            23090..23668
                     /gene="ygfB"
                     /locus_tag="ACHK3A_002669"
     CDS             23090..23668
                     /gene="ygfB"
                     /locus_tag="ACHK3A_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO8740726.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23694..25019
                     /gene="pepP"
                     /locus_tag="ACHK3A_002670"
     CDS             23694..25019
                     /gene="pepP"
                     /locus_tag="ACHK3A_002670"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO8740727.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            25016..26194
                     /gene="ubiH"
                     /locus_tag="ACHK3A_002671"
                     /gene_synonym="visB"
     CDS             25016..26194
                     /gene="ubiH"
                     /locus_tag="ACHK3A_002671"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO8740728.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGCVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWGPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAR
                     "
     gene            26217..27419
                     /gene="ubiI"
                     /locus_tag="ACHK3A_002672"
                     /gene_synonym="visC"
     CDS             26217..27419
                     /gene="ubiI"
                     /locus_tag="ACHK3A_002672"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO8740729.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(27493..>27571)
                     /locus_tag="ACHK3A_002673"
     CDS             complement(27493..>27571)
                     /locus_tag="ACHK3A_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740730.1"
                     /translation="KNTKSKIHNIRKYNAKTEVKLVYFK"
BASE COUNT         6583 a         6758 c         7366 g         6864 t
ORIGIN      
        1 ttaaaccgca cgagtatttt atttttaccg ccggacggtg ccctaaaaat aaaataagga
       61 ttatttatgc ggcttattga ttattttcca gaatcatcaa tctcagttat acattcagca
      121 aaagattggc aggaagctat cgatttctcg atggcatcat tgctggataa aaactatatc
      181 agcgagaatt acattcaggc aattaaagat tccaccatta acaatggccc ttattatatt
      241 ctcgcaccag gcgtggcaat gcctcatgct cgaccggaat gtggggcgct taaaaccggg
      301 atgtctttaa cattacttaa acaaggcgtt tattttccgg ggaatgacga accgataaaa
      361 ttactcatcg ggctttctgc tgccgatgcc gattcgcaca ttggcgctat tcaggcgtta
      421 agtgaattat tgtgtgaaga agaaatactc aaccaattat taacagcagc atcagaaaaa
      481 caattagcgg acataatcag ccgcggataa ttactctctc ctctacagga taatattatg
      541 gaaaacaagt ctgctcgtgc aaaggtccag gcttttgggg gctttttgac tgcaatggtc
      601 atccccaata tcggcgcttt tatagcctgg ggttttataa cggcgttatt tattcccacc
      661 ggctggctgc ctaacgaaca tttcgccaaa attgtcggcc cgatgattac ctatttattg
      721 cccgtgatga ttggttctac cggtggtcat ctggtcggcg gtaaacgcgg cgcggtcatg
      781 ggcggaatag gtactattgg tgtgatcgtc ggcgcagaga tcccgatgtt ccttggctca
      841 atgattatgg ggccgctcgg tggtctggtg attaaatata tcgacaaggc gctggaaaag
      901 cggatccctg ctggttttga gatggttatc aataacttct cattaggtat tgcggggatg
      961 ctcctttgtc tgctgggttt tgaagttatc ggcccggcgg tgttaattgc caatactttc
     1021 gtcaaagagt gtattgaggc gttggtacat gcgggttatc tgcctttgct gtcagtcatc
     1081 aatgaaccgg cgaaagtgct tttcctcaat aacgcgatag atcagggcgt ctattacccg
     1141 ttggggatgc aacaggcttc agttaacggt aaatccatct tctttatggt ggcgtccaac
     1201 cctgggcctg gcttaggact gttgctggcg tttactttgt ttggtaaagg gatgagtaaa
     1261 cgttctgcgc ccggggcgat gattattcac ttcctcggtg ggatccacga actgtatttc
     1321 ccgtatgtgc tgatgaagcc gctgaccatt attgccatga ttgcgggcgg tatgtctggc
     1381 acctggatgt ttaacttact ggacggtggt ctggtggctg gcccaagtcc ggggtctatc
     1441 tttgcttacc tggcactgac gccgaaaggc tcgttcctgg cgacaattgc cggtgttacg
     1501 gtaggtaccc tggtgtcctt tgctatcact tccgtaatac tgaagatgga aaaaacggtg
     1561 gaaacggaga gcgaagatga gtttgctcag tcagccaatg cggttaaggc gatgaaacaa
     1621 gaaggtgcat tctcgttaag cagggttaag cgtatcgcct ttgtttgcga tgcggggatg
     1681 ggctccagcg cgatgggcgc gaccaccttc cgtaaacgcc tggaaaaggc ggggctggca
     1741 atagaagtaa aacattacgc gatagaaaac gtgcctgcgg atgcggatat cgttgttact
     1801 catgccagtc tggaagggcg cgtgaaacgt gtgacggata aaccgctgat actgattaat
     1861 aactatattg gcgatccaaa actcgacact ttatttaatc aattaaccgc cgaacataaa
     1921 aactgattgc cgaggtaaaa atgaaaacca aagttgctgc tatttatggc aagcgggatg
     1981 tccgtctgcg cgaatttgaa ctgccagaaa ttaccgataa tgaactgctg gtgagtgtga
     2041 tttctgacag cgtgtgttta tccacctgga aagcggcgct actcggtagt gaacataaac
     2101 gcgtacccga cgatttagaa aatcatccgg tcattaccgg gcatgaatgc gccggggtta
     2161 ttgtcgaagt gggtaaaaat ctcaccgaca aatataaaaa aggccagcgt tttgtattgc
     2221 aaccggcaat ggggttacca agcggatatt cagcaggcta cagctacgaa tattttggcg
     2281 gtaacgccac ctatatgatt attcctgaaa tagccattaa cctgggctgc gtattaccgt
     2341 atcacggctc ttattttgct gcggcgtcgc tggcagagcc tatgtgctgc attattggcg
     2401 cttatcatgc caattatcac accacgcaat atgtttatga gcatcgcatg ggcgttaaac
     2461 ctggcggcaa tattgcgctg ctggcatgtg ctggtccgat gggcattggc gctatcgatt
     2521 atgctattaa cggcggcata caaccgtcgc gggtggtggt ggtcgatatc gacgacaaac
     2581 gcctggcgca ggtacaaaag ctgctgccgg tggaactggc ggccagtaaa ggcattgagc
     2641 tggtgtatat gaataccaaa gggatgagcg atccggtcca gacgctgcgg gcgctgacgg
     2701 atgatgccgg atttgatgac gtatttgtct atgcagcggt gccttcagtc gtcgagatgg
     2761 ctgatgaatt actggcggaa gatggctgcc tgaacttctt tgccgggccg acggataaaa
     2821 acttcaaagt gccgtttaat ttctacaacg tccattacaa cagcacgcat gtcgtcggta
     2881 cgtccggcgg ttcaacggac gacatgaaag aggcgattgc gcttagcgcc acagggcagt
     2941 tgcagccgtc gtttatggtg acccatatcg gtgggctgga tgcggtgcct gataccgtgc
     3001 tcaatctgcc ggatatcccc ggcggtaaaa aactcattta taacggcgtc accatgccgc
     3061 tcactgccat tgccgatttt gccgaaaaag gcaaaaccga tccgctgttt aaagagctgg
     3121 cgcggctggt tgaggaaacg cacggcatct ggaatgagca ggccgagaaa tatctgctgg
     3181 cgcaatttgg cgttgatatc ggggaggccg cgcaatgatg tccctggcgt ggccattatt
     3241 tcgcgttacg gaacaggcag cgttggctgc ctggccgcaa accggatgtg gcgacaaaaa
     3301 taaaattgat ggcctggcgg tcaccgcgat gcgccaggca ttaaacgacg tcgcttttcg
     3361 tgggcgagtg gttatcggcg agggggaacg atatcctctt tagcgctacg ggcgtgacgg
     3421 gcggcgagtt ggtgaacggt atccaacaga cggcgaatgg ggtgcggacg cagacattac
     3481 tgatcggcgg cgcggaccaa acgtgtaata taatagactc cctgcattga tggtgatcta
     3541 tcggaagacc aatggcgacg ctgacagaag atgatgtgct tgagcaactg gatgcacagg
     3601 acaatttatt ttcatttatg aaaactgcgc attctatttt gctccagggg atacgccagt
     3661 ttctgccgtc gctgtttgtc gataacgatg aagagatcgt cgaatatgca gtgaagccgt
     3721 tactcgccca aagcggcccg cttgacgata ttgatgttgc gctgcgtttg atttatgcgc
     3781 tggggaaaat ggataaatgg ctgtacgccg atatcacgca tttttcccag ttttggcatt
     3841 acctgaacga acaggatgaa acgcccggat ttgccgatga catgacctgg gattttatca
     3901 gcaacgtcaa tagcataacc tgcaatgcga cgctctatga cgcgttgaaa gcgatgaagt
     3961 tcgccgattt cgccgtctgg tcagaggcgc gttttagcag gatggtcaaa acggcgctga
     4021 cgctggcagt aacgaccacc ttaaaggaat taacgccgtg aaaattgaat taacggtgaa
     4081 tgggctgaag atacaggcac agtaccagaa cgaagagatt gaaaatgtac ataaaccgtt
     4141 gctgcacatg ttggcggcct tacagacggt aaatccgcag cggcgtacgg tggtttttct
     4201 ttgcgctccg ccggggacag gtaaatctac cctaaccacc ttctgggaat atctcgcaca
     4261 acaagacccg gaactgcccg ctattcagac actgccgatg gatggttttc accattacaa
     4321 tagctggctg gatgcgcatc aattgcgccc cttcaaaggc gcaccagaga cattcaacgt
     4381 tgcgaaactg gcggaaaatc tgtgccgggt tgtggaaggg gattgtacgt ggccgcagta
     4441 cgatcgacaa aagcatgatc ctgttgaaga tgcgttgcac gttaccgcac cactcgtcat
     4501 cgtcgaagga aactggctat tactggatga tgaaaagtgg caggagttgg catctttctg
     4561 cgatttttct atttttattc atgcacctgc tcaaattctg cgggagcgct tgattagtcg
     4621 taaaattgcg ggaggattat cgcggcaaga agccgaagct ttttatgccc gaaccgatgg
     4681 tcccaatgtc gagcgtgtgc tggtgaatag tcgtcaggcc aatctgatat tagagatgac
     4741 cgaagaaggc agatatcact tcaaaagctg acaatttatt ccagttattg caggggtatt
     4801 attacgccag cctggaataa agccccctac aaaaggggat aaaagtgtga tgtaagtcag
     4861 ataaatgtct tcttcggctg gacaatcatt ccttttattc cacgtttcgc ttatcctagc
     4921 tgaagcgttt cagtcgatta aatgttcgac aattaaccaa tcagtcgcag tttgcgacag
     4981 gtaaggtttc cccggacgat ttgctggatt actctgtctg gcaaatttgc tcagggaaaa
     5041 ccttgcagga gatctatgac cgtacgcgta gcgataaatg gcttcggtcg catcgggcgt
     5101 aatgtggttc gtgctttgta tgaatccgga cgccgggcgg aaattaccgt ggtggcaatc
     5161 aacgaactgg cggatgctgc gggcatggcg catttgttga aatatgacac cagccatggc
     5221 cgttttgcat gggaagtacg acaggaacgc gatcaacttt ttgttggtga tgacgccatc
     5281 cgtgtattgc atgaacgttc actgcaatcg ctcccctggc gtgaacttgg cgttgatgta
     5341 gtcctcgact gcaccggcgt atatggctcc cgcgagcatg gcgaagcgca tattgccgcc
     5401 ggggccaaaa aagtgctctt ttcacatcca ggcagtaacg atctcgacgc gaccgttgtt
     5461 tacggcgtca atcaggatca acttcgtgcg gaacaccgca tcgtttctaa cgcttcctgt
     5521 accacgaatt gcataattcc cgtcatcaaa ttgttagatg atgcgtacgg tattgagtcc
     5581 ggcactgtga ccacaattca ctccgccatg cacgatcaac aggttattga tgcataccat
     5641 cctgacctgc gtcgcacccg ggctgccagc cagtcgatca ttccggtcga tactaaactg
     5701 gccgccggta tcacacgatt ttttccgcaa tttaacgatc gctttgaagc gattgcggta
     5761 cgtgtgccaa ccataaatgt gacggcaatc gatttaagcg tgacggtgaa gaaacctgta
     5821 aaagccaatg aagtcaacct gttgctgcaa aaagcagcac aaggtgcatt tcatggtata
     5881 gttgactata cggaattgcc gttggtctct gtagatttta accacgatcc gcacagtgcc
     5941 attgtcgatg gcacccaaac ccgggttagt ggcgcacacc tgatcaaaac gttggtctgg
     6001 tgcgataacg aatggggctt tgctaaccga atgctcgaca cgacgttagc tatggctact
     6061 gttgctttca ggtaagacgc aagcagcgtc tgcaaaactt ttagaatcaa cgagaggatt
     6121 caccatgtct gtaattaaga tgaccgatct ggatcttgct gggaaacgtg tatttatccg
     6181 tgcggatctg aacgtaccag taaaagacgg gaaagtaacc agcgacgcgc gtatccgtgc
     6241 ttctctgccg accatcgaac ttgccctgaa acaaggcgca aaagtgatgg taacttccca
     6301 cctgggtcgt cctaccgaag gcgagtacaa cgaagaattc tctctgctgc cggttgttaa
     6361 ctacctgaaa gacaaactgt ctaacccggt tcgtctggtt aaagattacc tcgacggcgt
     6421 tgacgttgct gaaggtgaac tggttgttct ggaaaacgtt cgcttcaaca aaggcgagaa
     6481 gaaagacgac gaaaccctgt ccaaaaaata cgctgcactg tgtgacgtgt tcgtaatgga
     6541 cgcattcggt actgctcacc gcgcgcaggc ttctactcac ggtatcggta aattcgctga
     6601 cgttgcgtgc gcaggcccgc tgctggcagc tgaactggac gcgctgggta aagcactgaa
     6661 agaacctgct cgcccgatgg tggctatcgt tggtggttct aaagtatcta ccaaactgac
     6721 cgttctggac tccctgtcta aaatcgctga ccagctgatt gttggtggtg gtatcgctaa
     6781 cacctttatc gcggcacaag gccacgatgt gggtaaatcc ctgtacgaag ctgatctggt
     6841 tgacgaagca aaacgtctgc tgaccacctg caacatcccg gttccgtctg atgttcgtgt
     6901 agcaactgag ttctctgaaa ctgcaccggc taccctgaaa tctgttaacg atgtgaaagc
     6961 tgacgagcag atcctggata tcggtgatgc ttccgctcag gaactggctg aaatcctgaa
     7021 gaatgcgaaa accattttgt ggaacggtcc ggttggcgtg ttcgaattcc cgaacttccg
     7081 caaaggtact gaaatcgtgg ctaatgctat cgcagacagc gaagcgttct ccatcgctgg
     7141 cggcggcgac actctggcag caatcgacct gttcggcatt gctgacaaaa tctcctacat
     7201 ctccactggc ggcggtgcat tcctcgaatt cgtggaaggt aaagtactgc ctgcagtagc
     7261 gatgctcgaa gagcgcgcta agaagtaaaa aatcacaggg cagggaaacc tgcccttgtt
     7321 gcagcgcgct tttaaagcac gcactttttc atactctaaa taattcgagt tgcaggaagg
     7381 cgacaagaga gtgaatccca tgagcttaca taagtaagtg actggggtga gcgaacgcag
     7441 acgcagcaca tgcaacttga agtatgacga gtataaggcc cgacgataca ggacaagaga
     7501 catgtctaag atttttgatt tcgtaaaacc tggcgtaatc actggtgatg acgtacagaa
     7561 agttttccag gtagcaaaag aaaacaactt cgcactgcca gcagtaaact gcgtcggtac
     7621 tgactccatc aacgccgtgc tggaaaccgc tgctaaagtt aaagcgccgg ttatcgttca
     7681 gttctccaac ggtggtgctt cctttatcgc tggtaaaggc gtgaaatctg acgttccgca
     7741 gggtgctgct atcctgggcg cgatctctgg tgcgcatcac gttcaccaga tggctgaaca
     7801 ttatggtgtt ccggttatcc tgcacaccga ccactgcgcg aagaaactgc tgccgtggat
     7861 cgacggtctg ctggacgcgg gtgaaaaaca cttcgcagct accggcaagc cgctgttctc
     7921 ttctcacatg atcgacctgt ctgaagaatc tctgcaagag aacatcgaaa tctgctctaa
     7981 atacctggag cgcatgtcca aaatcggcat gactctggaa atcgaactgg gttgcaccgg
     8041 tggtgaagaa gacggcgtgg acaacagcca catggacgct tctgcactgt acacccagcc
     8101 ggaagacgtt gattatgcgt ataccgagct gagcaaaatc agcccgcgtt tcaccatcgc
     8161 agcgtccttc ggtaacgtac acggtgttta caagccgggt aacgtggttc tgactccgac
     8221 catcctgcgc gactctcagg aatatgtttc taagaaacac aacctgccgc acaacagcct
     8281 gaacttcgta ttccacggtg gttccggttc tactgctcag gaaatcaaag actccgtaag
     8341 ctacggcgta gtgaaaatga acatcgatac cgatacccaa tgggcaacct gggaaggcgt
     8401 tctgaactac tacaaagcga acgaagctta tttgcagggt cagctgggta acccgaaagg
     8461 cgaagatcag ccgaacaaga aatactacga tccgcgcgta tggctgcgtg ccggtcagac
     8521 ttcgatgatc gctcgtctgg aaaaagcatt ccaggaactg aacgcgatcg acgttctgta
     8581 agtcattcct ttctgcttat ctcaaggccc gctctgcggg tctttttttc gccaaaagca
     8641 aggtgatgca gatgagaaat ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc
     8701 ttgtcagact gcccgccata aggcggcgga gtgtatttct ccattttgag tcagttgaaa
     8761 aggaatattg aatggaagat ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc
     8821 tggtggctaa ccaggcgctg ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga
     8881 tcatcattgt tggtttgatt atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga
     8941 tctcccgtaa aatcgatgcc actgttgctg attttctctc tgcattagtc cgttacggta
     9001 ttatcgcctt tacgctaatc gctgcactgg ggcgcgtggg cgtacaaaca gcgtcagtca
     9061 ttgctgtact cggtgccgca ggcttagcgg ttggtctggc tttgcagggg tcactgtcta
     9121 acctggccgc tggcgtgtta cttgttatgt tccgcccgtt ccgtgccgga gaatatgttg
     9181 atctcggcgg cgtagccggt actgtgctga gtgtgcagat tttctccacc accatgcgta
     9241 ctgcagacgg taaaattatc gttattccga acggtaaaat tattgccgga aatattatta
     9301 acttctctcg cgagccagtt cgccgtaacg aatttattat tggcgtggcg tatgattccg
     9361 atatcgatca ggttaagcag atcctgaccg atattatcca gtctgaagat cgcattttga
     9421 aagatcgcga aatgactgtg cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg
     9481 tccgcgtctg gagcaacagc ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta
     9541 ttaaacgtga atttgatgcc gccggtatca gcttcccgta cccgcaaatg gatgtgaact
     9601 ttaagcgggt gaaagaagac aaagctgcgt aatcaacgct gaggccagat aatactctct
     9661 ggcctctctc ttattagttt ttctgattgc caattaatat tatcaatttc cgctaataac
     9721 aatcccgcga tatagtctct gcatcagata cttaattcgg aatatccaac gtgttttctt
     9781 attactttca aggtcttgca cttggggcgg ctatgatcct accgctcggt ccacaaaatg
     9841 cttttgtgat gaatcagggc attcgtcgtc agtaccacat tatgattgcc ttactttgcg
     9901 ctatcagcga tttggtcctg atttgcgccg ggatttttgg tggcagcgcg ttattgatgc
     9961 agtcgccgtg gttgctggcg ctggtcacct ggggcggcgt agccttcttg ctgtggtatg
    10021 gttttggcgc ttttaaaaca gcgatgagca gtaatattga gttagccagt gccgaagtcc
    10081 tgaagcaagg cagatggaaa attatcgcca ccatgctggc agtgacctgg ctgaatccgc
    10141 atgtttacct ggatactttt gttgtactgg gcagtcttgg cgggcaactt gatgtggaac
    10201 caaaacgttg gtttgcgctc gggacaatta gcgcctcttt cctgtggttc tttggtctgg
    10261 ctattctcgc agcctggctg gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca
    10321 atctggtagt gggatgtgtt atgtggttta ttgccttgca gctggcgaga gacggtattg
    10381 ttcatgcaca agccttgctc agttaggcgc tatctgatgg aaaaataaaa cagaggcgct
    10441 aagcttgcct ccagaggtcc tgaattttag gcaagtcaat taacacggag agactaacgt
    10501 gaagttcaaa gttatcgccc tggcggcatt aatgggtatt agcgggatgg cagcgcaggc
    10561 taacgaattg ccggatggac cgcatattgt cacctccggt acggcaagcg tggatgcggt
    10621 gccagacatt gccactcttg cgattgaagt caacgtggcc gcgaaggatg ccgctactgc
    10681 caagaaacag gcggatgagc gcgtcgcaca atacatttcc ttccttgaac tcaatcagat
    10741 cgcgaaaaaa gatatcagct cagcgaactt acgcacccag ccagattatg attatcagga
    10801 tggtaaaagt atcctgaaag gctaccgtgc tgtgagaacg gtggaagtca cgctccgtca
    10861 gttagacaaa ctgaattcct tgctggatgg cgcgctgaag gcgggtctta acgaaattcg
    10921 ttctgtatcg ctgggcgtgg cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc
    10981 gattgataac gcgattcatc aggcgcagga actggcgaac ggctttcatc gtaaactggg
    11041 gccggtatat agtgtgcgct accatgtttc caactatcag cccagcccaa tggtgcggat
    11101 gatgaaagcc gatgccgcgc cggtgtccgc ccaggaaact tacgagcagg ccgctattca
    11161 gtttgatgat caggtcgatg tggtcttcca gttagaacct gtggatcaac aacccgctaa
    11221 aacacctgca gcacaataat atttgtagcc cgacgtattc gtcgggctaa tgcctttacc
    11281 cgatattgcc tcctttttct gtaattttct gtaaaaccat gacgtaatca cctatctttg
    11341 ctcgcggttt catcaaggat aggctgactg aaacggttaa gcggacaagg atgcggggga
    11401 gggggtatgg acatttttat ttcgaaaaag atgcgcaatt tcattttatt agcgcaaacc
    11461 aataatattg cccgggcggc agaaaaaatc catatgacag cttcgccatt tggtaaaagc
    11521 attgccgcgc tggaagaaca aattggctat acgctattta cccgcaaaga taataatatc
    11581 agcctcaata aagccggaca ggaactgtat caaaaactgt tcccggttta tcaacggctt
    11641 tctgcgattg ataatgaaat ccataactca gggcgtcgtt cacgagaaat tgtgattggc
    11701 atagataata cttatccgac aattattttc gatcagttga ttagtctcgg agataagtat
    11761 gaaggcgtga ccgcccagcc agttgagttc agcgaaaatg gcgttatcga taatcttttt
    11821 gaccgtcagt tagattttat tatctcccca cagcatgtat ctgcacgagt tcaggaactg
    11881 gaaaacctga ctatcagtga actaccgccg ctgcgattgg gctttctcgt ttctcgtcgt
    11941 tatgaagagc gacaagagca agagttgctt caggagctac cctggctaca aatgcgcttt
    12001 cagaaccggg ctaacttcga agcaatgatt gatgcgaata tgcgtccttg tgggatcaat
    12061 cccaccatta tttatcgtcc ctacagtttt atggccaaaa tcagcgccgt ggagcgcgga
    12121 cattttctga cggtgatccc gcattttgcc tggcgactgg tgaatccggc aacgctgaaa
    12181 tattttgatg cgccgcacag gccgatgtat atgcaggaat atctttattc aatcagaaat
    12241 catcgttata ccgccacgat gcttcagcat attgctgaag atcgtgacgg gacgagtcat
    12301 taacccagca tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga
    12361 tcgtggtgaa tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac
    12421 ataggatgtg cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccacgc
    12481 agatcggcga tgccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca
    12541 tggctgcaca ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggggccata
    12601 aagatcgaca gatacgcgtt gcgttcaaaa tcaccgctgc caccgatgcc gttcatcaga
    12661 tcgaccccgg ctacgtgcgt tgagttggca tgcccgtaaa tatcaaactc caggccgacg
    12721 ttcagagcga tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga
    12781 cgcaacacaa tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg
    12841 gccgagatag tcaggctgga ggcgctggcc ccgctgattt tgcccgtttc cagtaaatgc
    12901 accaccgatt cctgtagcac ttccgaatac atcataaacg gaggaatttc cggattttcc
    12961 cccagacgcg ccattaccgc attattgatg ttgcctacgc cactttgcag cggcagaaat
    13021 tccggcggaa tacgcccatg cgccatttcc tgcaacaaga acgtgaccac gttatcggca
    13081 atctgctggc acatgggatt ttgcttatcc agcatattac cggcgtcagg caagttggtt
    13141 tccacgacgg cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc
    13201 attgcatgga agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct
    13261 gccagttctg caacgcgcgg atcgtgatag tgattgagtt caatgatcac tttcttcgcc
    13321 cgcagcagcc aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt
    13381 gccagtgccg atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc
    13441 atttgcgcca cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt
    13501 ttacgtaaac cggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa
    13561 agtacatcgt cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc
    13621 tttttggcct catgctgttc gttagctctg cgggcaatcg cgttgggtaa ggctttcggc
    13681 gaacccgctg gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc
    13741 gcttcatcgg cggtcatcct tgtccactga gtttccattt taactccttc gttcgcaggg
    13801 attaatgacc aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt
    13861 aatcttcgct gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt
    13921 tcatggtgtg cgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg
    13981 gcgctttctc agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt
    14041 ccacaacatg gttgaggatg ccgacagcca gcgcgcgctg ggctgtgatt ggcgaagcgg
    14101 taaaaatcag ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga
    14161 ccaggttata cgggacgccg aggtttacag gtgtcattga gaaggttgag gtactggcgg
    14221 cgatgatcag atcggaactc atgatcattt caaatgcgcc accccagaca ctaccttcca
    14281 ccatcgaaat gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaacggat
    14341 catcatagga gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg
    14401 agaagacttt ggaaccactc ggcgcgcgca aaatgataca gcgaatttcc ggccggttga
    14461 gatcgcttaa tgcctgcata agatcatcaa taaagacttt acttaaggca ttaagtttgc
    14521 ggccatagtt aaactcaatg accgccactt tgttgatagt gacaacgttc acatactgat
    14581 aagacataaa aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc
    14641 aggccggtgc gcggtgagag cgtattgttt ttgactgcta aaagcgtctg gcggtaatag
    14701 cgatcgaaat cttcattcgc gaacaggcga ttcagtactt cttcttcggt ctgcttacgc
    14761 agccattcca ccgattgttg ttgccgcact tgttgtaatc gaccactggc agttagcgcg
    14821 gttttgaagt cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta
    14881 caagtcagaa cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc
    14941 tcgtacatat gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata
    15001 acgatcagat ctgccacttc catcagcccc tttttaatgc cctgcagatc atcgccgcca
    15061 ccggcaattt gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttctgactgc
    15121 ccgacgccaa ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt
    15181 aattcccgcg ctcgctgact ggcaccgccc agatggccgg aggagggtac cgggcgaata
    15241 aacgccgcct cggcacgcgc aaggtcattc atgcgggttt tatccccgag aatgctaccg
    15301 ccagtgaccg ggctgctggg atcgaccgca ataaccgcaa cctttaatcc ctcacgaatc
    15361 aacaacatgc caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg
    15421 cccagtcgca gggcgttacc acagaacggc ataatggcgt caagcagctg tgtacttagc
    15481 gcctgatgac gcgggtgacg gctttccacc aacgtcatgg cctgggcgag cgtggcacgc
    15541 tcaccctgac gtaagcggcg aatactttct gctagcttgg cttcattaat catgatgctg
    15601 gcttatcaga ttcagtacgt cgcgcacgct gtcgagcata ggtgtacctg gaccataaat
    15661 cgccgccacg ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc
    15721 gaccacgcag atatctttgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag
    15781 cgttttatga ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc
    15841 caggcgggcg atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag
    15901 atcggaatag gcgctggcga tcactttcgc gccgcgatcg tgcccatcct ggcccatttt
    15961 ggcgatcaga atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac
    16021 aatggcatcg aactcggcgg ccgatttctc agactgatga tagctttgcg caatcacgcc
    16081 ggtaacacac tggcttggca ccagataacg gtcgaaagcg gcttccagcg catcggaaat
    16141 ttcacccagg gtggcgcgaa cgcgagcagc attaacagcg gcagccagca ggttttcgtt
    16201 atgctgtgcg gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt
    16261 ggcgcgaatg cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc
    16321 aagtacatcg gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg
    16381 cttgccctgg tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg
    16441 cagacctgct tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc
    16501 tctggcttgt ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg
    16561 atcgacggtg cggcagagtt ctgattcttc ctgaatgatg atctgggtat tgcgggcaat
    16621 gcgtgctgag aaatcggtag gcaaaccaag cgcttcatca aaggcgttgg tatgcagtga
    16681 ctgagtaccg cccagcgttg cagccagtgc ttcaatggtg gtgcggataa cgttgttata
    16741 cggatcctgt tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga
    16801 tttcggatcc tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg
    16861 caacatggcg acgttcataa acagatccat gccaatgccg aagaagaacg acagacgagg
    16921 agcgaagtca tcaattttca gcccggcgga gattgctgct ttgatgtact caatcccatc
    16981 ggcgagcgta aatgctacct gctgcacgca gttggcaccc gcttcaccca tgtggtaacc
    17041 gctgatactg atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtctgc
    17101 gataatacgc attgacggtt ttggcgggta aatataggtg ttgcggcaaa gatactcttt
    17161 gaggatatcg ttttgaatgg tgccggtcag tttatcgggt gtaacacctt gctcttctgc
    17221 ggcgacaata taaaacgcca gtactggtag tactgcgcca ttcatggtca tcgaaaccga
    17281 cattttatcc agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc
    17341 aacgcccgct ttgccgacgt cgcccgccac gcgtgggtta tcggagtcgt agcctcggtg
    17401 ggtggcaagg tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata
    17461 aaaagcgttg gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg
    17521 ggcggtatac atagtggcac gcgggccacg aacgtaggga ggcaaaccag gaagggtacc
    17581 tgtcacctcc agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc
    17641 cgcggtttgc tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc
    17701 aagctgttgc cactcctgca ccttagacat tatttgctcc gtaaaacgca atcaaaatga
    17761 gggtatttgc gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat
    17821 tcagacgtcg ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac
    17881 aaaaagaagt gaggataacg cctgatatgt gctatcaggc attatttgat ggattaatcc
    17941 tgacgaagga ctttgtgacc ataatcgagc aacgcatcag tgactttacg catcatgcgg
    18001 ctttcaggag caaagcggtg ccagtagagc atccgtcgtt gaaatagccc tggcgttaag
    18061 tcaatcagtt caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag
    18121 gtggtgccct gacgagcaag ttgcacgaac gcttccgaag aattaacgat atggcagggt
    18181 acgctgccag gaggcagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga
    18241 tggtcaaaag cgaccacagg tgctttcaat aatgccgaac gcgttacgcc gttagggaaa
    18301 tatttttcgg caaacggttt tgagctgacg aacagatagt cgagcgcacc gagcttatcg
    18361 acaaggcaac tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg
    18421 cgcagacgtt cctgagtgcg ggtttcatct tctacctgca agttgaggcg gataggcgaa
    18481 tcagctaaca caggagccag cgccggaagc aaccacgtcg ccagactgtc agcgttgacc
    18541 gccagtgaaa gcagcagcgg agttgaaccg gtttgttcat cgcccagcca ctcttcttcc
    18601 agtaactcca cctggcgtag cagtgccagc agtttttgcc cttgttctgt cgggcgcggt
    18661 ggtacggtac gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt
    18721 gagacggctg attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt
    18781 atcaccgcat ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc
    18841 tcctggtaaa tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga
    18901 agtcgttggc gggtaacatt tgtatcagtg gttaacgcgc agcttaaaag atcagggatt
    18961 tgcgctaatg catttcgctc agcgagttcg cgccctggtt gtgttgaatg gcgtggcgtt
    19021 attgcctctg ttcgcatcgt ggagcggcat acccagaccg ttgtatgctt tatttgcggg
    19081 gcgaaagggg atgcccgcca ttgcgcgata ttttatggat gagttaacca cccggcttgc
    19141 caacggggtc tgaatcgctt tttttgtata taatgcgtgt gaaatttcat accacaggcg
    19201 aaacgatcat gacgcaggat gaattgaaaa aagcagtagg atgggcggca cttcagtatg
    19261 ttcagcccgg caccattgtt ggtgtaggta caggttccac cgccgcacac tttattgacg
    19321 cgctcggcac gatgaaaggc cagattgaag gggccgtttc cagttcagat gcttccactg
    19381 aaaaactgaa aagcctcggc attcacgttt ttgatctcaa cgaagtcgac agccttggca
    19441 tctacgttga tggcgcagat gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg
    19501 cggcgctgac ccgtgaaaaa atcattgctt cggttgcgga aaaatttatc tgtattgccg
    19561 acgcctccaa gcaggttgat attctgggca aattcccgct gccagtagaa gttatcccga
    19621 tggcacgtag tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg gaataccgtc
    19681 agggcgtggt gaccgataac ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg
    19741 acccgatagc gatggaaaac gccataaatg cgattcctgg cgtggtgact gttggcttgt
    19801 ttgctaaccg tggcgcggac gttgcgctga ttggcacacc tgacggtgtc aaaaccattg
    19861 tgaaatgatc tgacggggga acctcccccg ttaaaaaaat tctcttcatt aaatttggtg
    19921 acatgtgtca cgtttttacc tggctattgt cgattgctct aaataaaccc tctaaaccag
    19981 catattcatc caagaaatac ctttgcgtga tatttcctca atatcgtgac gcaaacgttc
    20041 atattgccgc aatattattt tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca
    20101 tttcaaaaga caggattggg taaatggcaa aggtatcgct ggagaaagac aagattaagt
    20161 ttctgctggt agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca gcgggttaca
    20221 ccaacatcga atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg
    20281 atgcccactt catcggcctg cgatcccgta cccatctggc tgaagacgtg atcaacgccg
    20341 cagaaaaact ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt gatctggatg
    20401 cggcggcaaa acgcgggatc ccggtattta acgcaccgtt ctcaaatacg cgctctgttg
    20461 cggagctggt gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta
    20521 aagcgcatcg tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa
    20581 agctgggtat catcggctac ggtcatattg gtacgcaatt gggcattctg gctgaatcgc
    20641 tgggaatgta tgtttacttt tatgatattg aaaacaaact gccgctgggc aacgccactc
    20701 aggtacagca tctttctgac ctgctgaata tgagcgatgt ggtgagtctg catgtaccag
    20761 agaatccgtc caccaaaaat atgatgggcg cgaaagagat ttcgctaatg aaacccggct
    20821 cgctgctgat taatgcttcg cgcggtactg tggtggatat tccagcgctg tgtgacgcgc
    20881 tggcgagcaa acatctggca ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca
    20941 atagcgatcc atttacctct ccgctgtgtg aattcgacaa tgtccttctg acgccacaca
    21001 ttggtggttc gactcaggaa gcgcaggaga atatcggtct ggaagttgcg ggtaaattga
    21061 tcaagtattc tgataatggt tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc
    21121 cactgcacgg tggtcgtcgt ctgatgcaca tccacgaaaa ccgtccgggt gtgctgactg
    21181 cgctcaacaa aatttttgcc gagcagggcg tcaacatcgc cgcgcaatat ctgcaaactt
    21241 ccgcccagat gggttatgta gttattgata ttgaagccga cgaagacgtt gccgaaaaag
    21301 cgctgcaggc aatgaaagct attccgggta ccattcgcgc ccgtctgctg tactaaatcc
    21361 ccttatctga aaatcaacgg gcaggtcact gacttgcccg tttttttatc ccttctccac
    21421 accgttgccg cattgcgcat tctactttgc gagtctgctc cagaaaataa atcaaagtca
    21481 gatcaatgcg ttgtgtttat ttaatgggga tttacgatgg cagggcagca tggggctgta
    21541 acggtaaagc cctcaccgaa gcgagggctt gaaggagaag ggttatgatg cgacttgtca
    21601 tcatactgat tgtactgtta ctcataagtt tcccagctta ttaacagtca gtctcagggg
    21661 aggagtcatc ctcccttacc cttactcact aaattaggtt aaagaatcaa cgatgtcaat
    21721 cagggcgatg caggtgtgtc gccctcacca ctcccagact ttcgacggtg taaccaccgc
    21781 aggaagaggg atatcccact cttcaacggg gagtttttcc accaactgac aatcatgcgc
    21841 ataacccacc ggttgcattt tatagtgctg ccagttttgt aaggtccgat cataaaaacc
    21901 accgcccatt cccaggcgct gaccgtactc atcaaaggcg accagcggtg tgatcaacac
    21961 gtctaatcgg gaaaggggta gcacgtcacg cacatccagt ttgggctcat ggatcttcaa
    22021 cctgttcatc accagttcgc tttgcggatg gtaattcagg aacagcaaat taccggcact
    22081 gaagggatgc aaaactggaa gatatacgcg cttaccggcg cgccagagtt gttctatgag
    22141 tggcagggtg tcgagttcgc catcaaaaga gaggaataca gcgaccgtat gtgccatcac
    22201 caccggggga taagtcatca tccgggtagc ggcttgttga cccatttcct gctgttgttc
    22261 cggcgttaac gcacgacgac gttgccgaat cattttgcgg atttcttgtc gggataatgt
    22321 cagtgggagt tctggtagtt gcgtcatggc tggtgccaga taagaaggga atctccgaga
    22381 tgccgccgca ggctgtaacc cttgaaccct tggttcaagg tgaatgtgtc gtcgcagttt
    22441 taaggcttct cggacggacc gagcatgctc accaaccgcg gagcgccaca ttcttgtggt
    22501 atgaaatatc ggctcagggg actggcccgc ttgcgaacat ctcagagaaa ttttgtcttc
    22561 acggttactc taccacagta aaccgaaaag tgtcattcaa agttttggtt agttttttcg
    22621 gtgatgcgac cttgttcaaa taacgcttgt tctatggtct gctgcagcat ccgaatacgc
    22681 tgttccatac ttgccgcata gtcacgagtc ttcgcttttt cttgcgctaa ctcatagctg
    22741 atattcaatg cggcaatgaa gaccaactgt tcagtatttg tgactctagt gcgttctttc
    22801 agatcttgca accgttggtt cagatcgtcc gctgcctgat tcaacgcatc cctttggtca
    22861 ggcgggcagt tcacacgcag tgaacggcca aaaatttgga tatcgacggg ttgtgcagac
    22921 atgccacctt cctgctgatt gactgcgctg ccttcgtctt tagacccatg gtctgcgaag
    22981 gggcgacact atagctaccc tgatgagaag agacaagccc ttttctggtc caccagggcc
    23041 caaagtggta gcatatcatg aatattcctc cctttgacga cgaatgctta tgtctataca
    23101 gaacgaaatg cctggttaca acgaaatgaa ccagtatctg aaccaacaag ggacgggtct
    23161 gacccctgct gagatgcatg gtttaatcag cgggatgata tgtggcggta acgatgacag
    23221 ctcatggctg ccgctacttc acgacctgac gaacgaaggc atggctttcg gtcatgagct
    23281 ggcacaggca ctgcgtaaaa tgcactctgc caccagcgat gccctgcagg atgacggctt
    23341 cctttttcag ctttatctgc ctgatggcga tgatgtcagc gttttcgatc gggctgatgc
    23401 gctggcaggt tgggtcaatc acttcctgct tggtcttggc gttacgcaac cgaagctgga
    23461 caaagtgacc ggagaaaccg gtgaagccat cgacgatctg cgtaacatcg cgcagctggg
    23521 ctacgacgaa gacgaagatc aggaagagct tgaaatgtcg cttgaagaga tcatcgaata
    23581 cgttcgtgtt gccgcgctgt tatgccacga cacctttact catccgcaac cgaccgcgcc
    23641 agaagtacaa aaaccgactc tacactaaaa acaaaaaacg taaggagagt gttatgagtg
    23701 agatatcccg gcaagagttt cagcgtcgcc gtcaggccct ggtggagcaa atgcaacccg
    23761 gcagcgccgc gctgattttt gctgcaccag aagtaacacg tagcgccgac agcgaatacc
    23821 cctatcgtca gaacagtgac ttctggtact tcaccggctt taacgaaccg gaagcggtgc
    23881 tggtgctgat taaaagcgac gacactcata accacagcgt tttgtttaac cgcgttcgcg
    23941 acctgacggc agagatctgg tttggtcgtc gcttaggcca ggatgccgcg ccagagaaac
    24001 tgggcgttga ccgcgcactg gcattcagcg aaatcaatca gcaactttat caactgctta
    24061 atggtctgga tgtggtttat cacgctcagg gcgaatatac ctatgctgat gaaatcgtga
    24121 acagcgcgct ggaaaagctg cgtaaaggct cgcgacagaa tctcactgcc ccggctacga
    24181 tgatcgactg gcgtcctgtt gttcatgaaa tgcgcctgtt caaatcacca gaagagattg
    24241 ccgtacttcg ccgcgcgggt gaaatcaccg cactggcgca tacccgggcg atggaaaaat
    24301 gccgtccggg gatgttcgag taccatctgg aaggcgaaat tcaccacgag tttaaccgcc
    24361 acggtgcgcg ttacccttcc tataacacca ttgttggcag cggtgaaaac ggctgcattt
    24421 tgcactacac cgaaaacgag tgtgaactgc gtgacggtga tctggtgttg attgacgcgg
    24481 gttgtgaata caaaggttac gctggcgaca ttacccgcac cttcccggtc aacggcaaat
    24541 tcactcaggc tcagcgtgaa atctatgaca ttgtgctgga gtctcttgaa accagcctgc
    24601 gcctgtatcg tccgggaact tccatgcagg aagtcactgg tgaagtggtg cgtatcatgg
    24661 ttagcggcct ggtaaaactc ggcatcctga agggtgatgt tgatgaactg atcgctcaga
    24721 acgcccatcg tcctttcttt atgcatggcc ttagccactg gttaggactg gatgtccatg
    24781 acgttggcgt ttatggtcag gatcgctcgc gcattctgga accggggatg gtactgaccg
    24841 tagagcctgg tctgtatatc gcgccggatg cggatgtgcc agaacaatat cgcggtatcg
    24901 gcattcgtat tgaagacgac attgtgatta ccgaaaccgg caacgaaaac ctcaccgcca
    24961 gcgtggtgaa aaagccggaa gaaatcgaag cgttgatggc tgctgcgaga aagcaatgag
    25021 cgtaatcatc gtcggtggcg gcatggcggg cgcgacgctg gcgctggcta tttcccggtt
    25081 aagtcacggg gcgctgccgg tacatttgat tgaagcgact gcgccagagt cacatgctca
    25141 tccgggcttt gatggacgag cgatagcgct ggcggcgggt acctgtcagc aactggcgcg
    25201 catcggcgtc tggcaatctc tggcggattg cgcaaccgcc atcactaccg tgcatgtcag
    25261 cgatcgtggt catgccggat tcgtcaccct cgccgcagaa gattaccaac tggcggcgct
    25321 ggggcaggtt gtcgaattgc ataatgtcgg gcaacggctg tttgcattgc tgcgcaaagc
    25381 acctggcgta acgctgcatt gtcctgatcg cgtggctaac gttgcccgta ctcagagtca
    25441 cgttgaagtg acgctggaga gtggcgagac gctgacgggc tgcgtgctgg tagccgctga
    25501 tggcacccat tcagcgttag ccaccgtgtg cggcgttgac tggcagcagg agccttacga
    25561 acaactggct gtgattgcca acgttgcgac ttccgttgcg catgaagggc gcgcttttga
    25621 acgctttaca caatatggcc cgctggcgat gttgccgatg tctgacggac gctgttcgct
    25681 ggtctggtgt catccactgg aacggcgcga agaggtactg tcttggagcg acgagaagtt
    25741 ttgccgtgaa ctccagtcgg cctttggttg gcgacttggg aaaattaccc acgctggtaa
    25801 acgcagtgct tatccgctgg cgttaacccg cgccgccaga cctattaccc atcgtaccgt
    25861 gctggtgggc aatgcggcgc aaactctgca cccgattgcc gggcaagggt tcaacctcgg
    25921 tatgcgagat gtcatgagcc ttgcggaaac cctgactcag gcgcaggagc gcggagaaga
    25981 catgggtgat tacggcgtat tgtgccgtta tcagcagcgt cgacagcgcg atcgcgaagc
    26041 aaccatcggc gtcacggaca gccttgtaca tctttttgcc aaccgttggg ggccgctggt
    26101 tgtcgggcgc aacatcgggc tgatggcaat ggaattattc accccagcac gcgatgtgct
    26161 ggcgcagcgc accctcgggt gggtggcgcg ttgaaggcgg ttttaaggaa taaacaatgc
    26221 aaagtgttga tgtagccatt gttggcggcg gcatggtggg gctggcggtt gcctgcggct
    26281 tgcagggtag cggcttacgc gttgccgtac tggagcagcg cgtaccggaa cctctggcgg
    26341 cggatgcacc accacaactg cgcgtttcgg ctatcaatgc cgccagcgaa aaattactca
    26401 cccgtcttgg tgtctggcag gacattctct ctcgtagggc cagttgttat cacggtatgg
    26461 aagtgtggga caaagacagt tttggtcaca tttcgtttga cgatcaaagc atgggctata
    26521 gccatcttgg gcatatcgtt gaaaactcgg tgattcacta cgcgctgtgg aacaaagcgc
    26581 agcagtcgtc agatatcact ctgttggccc ccgcagaatt acagcaggtc gcctggggag
    26641 aaaatgaaac cttcctgacg ttgaaagatg gcagtatgtt aacggcgcgt ctggtgattg
    26701 gcgcggacgg cgctaattcc tggttgcgca acaaagccga tattccgctg actttctggg
    26761 attatcagca tcacgcgctg gtagcgacca ttcgcacgga agaaccgcat gatgcggtgg
    26821 cgcggcaggt ttttcatggc gaaggcattc tggccttttt gccgcttagc gatccgcatc
    26881 tttgctcgat tgtctggtca ctgtcgccag aggaagcgca gcggatgcag caggcaggcg
    26941 aagacgaatt taatcgcgcg ttaaatatcg cttttgataa tcgcctgggc ttatgcaagg
    27001 ttgaaagtga gcgcctggtg ttcccgctaa cggggcgtta tgcgcgccag tttgccgcac
    27061 accgtctggc gctggtgggc gacgccgcgc atactattca cccgctggcg gggcaggggg
    27121 taaatctcgg ctttatggat gctgcagagc tgattgctga actgaaacgg ttgcatcgtc
    27181 agggtaaaga catcgggcag tacatttacc tgcgtcgcta tgagcgtagc cgtaagcaca
    27241 gtgcggcgct gatgctggct ggtatgcagg gattccgcga tctgttttcc ggtaccaatc
    27301 cggcgaaaaa attgctgcgt gatattggtc tgaaactggc tgatacgctt cctggcgtta
    27361 agccacaact tattcgtcag gcaatgggat taaacgattt gcctgaatgg ctgcgttaaa
    27421 tatttctctt ctgctgttta tttgataccc atcacacttt catctcccgg ttttttcgcc
    27481 gggagatttt cctcatttga aataaactaa tttaacctcc gttttcgcat tatattttct
    27541 aatgttatgg atttttgatt tagtgttttt t
//
LOCUS       DAZGHF010000062        27497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGHF010000062 DAZGHF010000000
VERSION     DAZGHF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.062"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            65..322
                     /gene="bhsA"
                     /locus_tag="ACHK3A_002674"
                     /gene_synonym="ycfR"
     CDS             65..322
                     /gene="bhsA"
                     /locus_tag="ACHK3A_002674"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO8740731.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(405..1367)
                     /gene="ldtC"
                     /locus_tag="ACHK3A_002675"
     CDS             complement(405..1367)
                     /gene="ldtC"
                     /locus_tag="ACHK3A_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO8740732.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1511..4957)
                     /gene="mfd"
                     /locus_tag="ACHK3A_002676"
     CDS             complement(1511..4957)
                     /gene="mfd"
                     /locus_tag="ACHK3A_002676"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO8740733.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5085..6158)
                     /gene="ycfT"
                     /locus_tag="ACHK3A_002677"
     CDS             complement(5085..6158)
                     /gene="ycfT"
                     /locus_tag="ACHK3A_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO8740734.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMTSLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6420..7619
                     /gene="lolC"
                     /locus_tag="ACHK3A_002678"
     CDS             6420..7619
                     /gene="lolC"
                     /locus_tag="ACHK3A_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO8740735.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7612..8313
                     /gene="lolD"
                     /locus_tag="ACHK3A_002679"
     CDS             7612..8313
                     /gene="lolD"
                     /locus_tag="ACHK3A_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO8740736.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8313..9557
                     /gene="lolE"
                     /locus_tag="ACHK3A_002680"
     CDS             8313..9557
                     /gene="lolE"
                     /locus_tag="ACHK3A_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO8740737.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9586..10497
                     /gene="nagK"
                     /locus_tag="ACHK3A_002681"
     CDS             9586..10497
                     /gene="nagK"
                     /locus_tag="ACHK3A_002681"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO8740738.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10513..11334
                     /gene="cobB"
                     /locus_tag="ACHK3A_002682"
     CDS             10513..11334
                     /gene="cobB"
                     /locus_tag="ACHK3A_002682"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO8740739.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11460..12245)
                     /locus_tag="ACHK3A_002683"
     CDS             complement(11460..12245)
                     /locus_tag="ACHK3A_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFO8740740.1"
                     /translation="MKKIIILLSLLILLPLTVTSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHAASSMSNVKGSVLLLFTTRE
                     PRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDLPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAVA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(12242..12703)
                     /gene="ymfA"
                     /locus_tag="ACHK3A_002684"
     CDS             complement(12242..12703)
                     /gene="ymfA"
                     /locus_tag="ACHK3A_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO8740741.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPYSKKTRRKRKSRP"
     gene            complement(12761..13807)
                     /gene="potD"
                     /locus_tag="ACHK3A_002685"
     CDS             complement(12761..13807)
                     /gene="potD"
                     /locus_tag="ACHK3A_002685"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO8740742.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(13804..14598)
                     /gene="potC"
                     /locus_tag="ACHK3A_002686"
     CDS             complement(13804..14598)
                     /gene="potC"
                     /locus_tag="ACHK3A_002686"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO8740743.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(14595..15452)
                     /gene="potB"
                     /locus_tag="ACHK3A_002687"
     CDS             complement(14595..15452)
                     /gene="potB"
                     /locus_tag="ACHK3A_002687"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO8740744.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(15436..16572)
                     /gene="potA"
                     /locus_tag="ACHK3A_002688"
     CDS             complement(15436..16572)
                     /gene="potA"
                     /locus_tag="ACHK3A_002688"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO8740745.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPADEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFTVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            16822..18051
                     /gene="pepT"
                     /locus_tag="ACHK3A_002689"
     CDS             16822..18051
                     /gene="pepT"
                     /locus_tag="ACHK3A_002689"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO8740746.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            complement(18197..19318)
                     /gene="roxA"
                     /locus_tag="ACHK3A_002690"
                     /gene_synonym="ycfD"
     CDS             complement(18197..19318)
                     /gene="roxA"
                     /locus_tag="ACHK3A_002690"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO8740747.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETSWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(19394..20854)
                     /gene="phoQ"
                     /locus_tag="ACHK3A_002691"
     CDS             complement(19394..20854)
                     /gene="phoQ"
                     /locus_tag="ACHK3A_002691"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO8740748.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(20854..21525)
                     /gene="phoP"
                     /locus_tag="ACHK3A_002692"
     CDS             complement(20854..21525)
                     /gene="phoP"
                     /locus_tag="ACHK3A_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO8740749.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(21694..23064)
                     /gene="purB"
                     /locus_tag="ACHK3A_002693"
     CDS             complement(21694..23064)
                     /gene="purB"
                     /locus_tag="ACHK3A_002693"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO8740750.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(23068..23709)
                     /gene="hflD"
                     /locus_tag="ACHK3A_002694"
     CDS             complement(23068..23709)
                     /gene="hflD"
                     /locus_tag="ACHK3A_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO8740751.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(23745..24851)
                     /gene="mnmA"
                     /locus_tag="ACHK3A_002695"
                     /gene_synonym="trmU"
     CDS             complement(23745..24851)
                     /gene="mnmA"
                     /locus_tag="ACHK3A_002695"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO8740752.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYDGEVCLGGGIIEQRLPLPV"
     gene            complement(24905..25366)
                     /gene="nudJ"
                     /locus_tag="ACHK3A_002696"
     CDS             complement(24905..25366)
                     /gene="nudJ"
                     /locus_tag="ACHK3A_002696"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO8740753.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(25376..26029)
                     /gene="rluE"
                     /locus_tag="ACHK3A_002697"
     CDS             complement(25376..26029)
                     /gene="rluE"
                     /locus_tag="ACHK3A_002697"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO8740754.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTEDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            26201..27451
                     /gene="icd"
                     /locus_tag="ACHK3A_002698"
     CDS             26201..27451
                     /gene="icd"
                     /locus_tag="ACHK3A_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO8740755.1"
                     /translation="MESKVVVPAQGKKITLQNSKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         6702 a         7009 c         7128 g         6658 t
ORIGIN      
        1 tcatttagtt atcgatcgtt aagtaattgc ttgcgacgtc attcatctgc ataaggccac
       61 tattatgaaa aacgtaaaaa ccctcatcgc tgcggcgatt ttaagctcaa tgtcatttgc
      121 cagctttgcg gctgtcgaag ttcagtcaac gccagaaggc caacaaaaag tcggtactat
      181 cagtgctaac gcggggacaa atctgggatc gctggaagag cagctggcgc aaaaagcgga
      241 tgagatgggc gcaaaatctt tccgtattac ttctgtaacc ggtccgaata ccctccatgg
      301 aacagcagta atttataaat aagcattaac cctcattaat gcctgctact gctgattttt
      361 ttccccgcga catgccgtgt cgcggggatt tttttatccg agctttacag agtttgtggg
      421 ctcacttgat gacggcggac atccaccggc atcccggaac ggacatccat cacatgttgc
      481 atcacttcag cgtcagtttg tgctgcatct ttaaatgatt gcattgcgct attcagcgta
      541 attggcagca gctgcggatc gtcatcaatc ttctctgaca gcggctgatg tacttcaacc
      601 agacgcgcac cgtttggttc ggcagagact tttatcggag tgttgatgat attcactttg
      661 gtgcctgggg tgacctggct aaagagtgtt ttgatatcgt catcccgcag acgaatacag
      721 ccagaactta cccgcatgcc aatgccgaaa tcggcgttcg taccgtggag caaataaacg
      781 ccgccatagg ccgccaggcg aatcgcatga tggcccattg gattatccgg tccagccgga
      841 actaccgcag gcaactcaat tccctgtgct ttataacggg cgcggatgtt tgccgttggc
      901 gtccaggttg ggtttgcacg tttgtctgaa acggtggtca ccattgtcgg cgtcagcgtg
      961 tcaccaccta actgaccaat gccgatggga tacacggtta ccgaattttt acccggcggg
     1021 tagtaataga gacgcagttc cgcaatattg atcacaatgc cttcgcgcgg cgcatctgga
     1081 agtagggttt gcaacgggat cgttaacacg ctgcccgcgc gcggtacgta aggatcaacg
     1141 ccggggttag cctgtaacag agcgagaaag ccgacgttat attttttggc gatggcttcc
     1201 agagaaccac cgtcattttc caccacatga aatttgtttt cgccaaccag acgactgccc
     1261 gctggcggca gcggccaggt gttggctttt gccggtagcg ccagcgccac ggcagcggcg
     1321 aacgtaaaaa acgttagcca gcgagaaaaa cgcgttttga tcatcaccaa aaatccataa
     1381 taaatataag gttattgtaa taaacagata agcctgaatt atggatggtg acagtgtcgg
     1441 atagtgcagg gaagtgcaaa gaatttgtaa atgttgcaga tgggggcgca gaaacgcccc
     1501 cgatttacca ttaagcgatc gcgttctctt ccagttcacg cataaactgg cgtacccatt
     1561 cgatacgcgt tttccgctca ctcaaatcct gaataaactt caggcgcgtc ggaccatcga
     1621 ggcgataatg ctgcggctgt ttttgcagca aaccaatcaa ccaggccgga ttaacgtgat
     1681 tcttctcggc aaattcgatc acgccgcctt tctcattacc ttccagcttc ctgatcccca
     1741 gtttctgcgc ttgctggcgc agacgggcaa tatccagcag ggtacgcgcc ggatccggca
     1801 gcaggccgaa gcgatcgata agctcgactt tgatctcttc cagttcgttt tccgttttgg
     1861 cgctggcaat acgtttgtag aacgacagac gcgtgttcac gtcagggatg aaatcatcgg
     1921 gcaatagcga cggcatccgc agctcgactt ctgtttgctg actggtgaga tcttccagcg
     1981 acggctcgcg tccggctttc agcgcatcga cagcgttttc cagcaactcc atatacagcg
     2041 agaaaccgat ggtttccatt gagccgcttt gttcttcgcc aagcagttca cctgcgccgc
     2101 ggatctccag atcgtgcgtt gccagcgcaa aacctgcccc gagatcttcc agcgaggcaa
     2161 tcgcttcaag acgtttttgc gcatcggtag tcatcgcttt tggatgcggc gtcagcagcc
     2221 atgcatatgc ctggtggtgc gaacgcccga cgcgaccgcg taactggtgc aactgtgcca
     2281 gaccgaagtg atccgcgcgt tcaatgataa tggtgttggc ggtaggaatg tcgatcccgg
     2341 tttcgataat ggtggtacaa accagcacgt tgaaacgttg atgatggaaa tcattcatca
     2401 cccgttccag ttcgcgctca cgcatttgcc cgtgaccgat ggcaatccgc gcttcaggta
     2461 ccagttctgc cagccgttcg gcggctttct ggatgttttc cacatcattg tagagataat
     2521 aaacctgtcc tccgcgcaaa atttcacgca ggatcgcctc ccggaccacc agactgtcat
     2581 actcacggac aaaggtttta actgccagac gacgggcggg cggcgtggcg ataatcgaca
     2641 ggtcacgcat tccgctcatt gccatattca gcgtacgtgg gatcggcgtt gcagtaagcg
     2701 tcagaatatc cacgttcgcg cgcatcgctt taatgcgctc tttatgacgc accccgaagc
     2761 ggtgttcttc atcgacaatc agcaggccta aatctttaaa cttgacgtca ctttgcagca
     2821 gtttgtgcgt accgatcaga atatcgattt tcccttccgc tacttccgca aggatttgcg
     2881 tctgctcttt ggcgctgcgg aaacgggaga tcatttcgat acgtaccggc cagttggcga
     2941 aacggtcgcg gaagttgtcg taatgctgct gcgcgagaag ggtggttggc accagcaccg
     3001 ccacctgctt gtggttatct actgccagga aagcggcgcg catcgccact tctgttttac
     3061 caaagccaac atcgccgcac accagacgat ccattgccag cggctgacac atgtcgctaa
     3121 gtaccgcatt aatggcctgt gcctgatccg gcgtggtttc aaacgggaag ctgtcgcaga
     3181 acaactgata ctgctcgcga tcgtgtttaa acgcgaagcc ctctttggcg gcgcgttgcg
     3241 cgtagatatc cagcaattct gctgccacat cacgcacttt ttccgccgct ttctgccgcg
     3301 cgcgtgacca ggcatcgccg ccaagtttat gcagcggggc gttttcttcc gcgccacccg
     3361 cgtaacggct aatcagatgc agtgacgaca ccggaacata cagtttggcg tcgttggcat
     3421 aggtgagcat caggtactcg ccagtaatac cgcccgcttc cagcgtggtc attccggcat
     3481 agcgcccgac accgtgctcc agatggacca ccggctgacc aatatgcagc tccgcgaggt
     3541 tacggatcag tgtatcgggg ttgatggcgc ggcgagaatc ctgacgacga cgggcaacgc
     3601 gttcaccgag cagatcgctt tcgcaaatca gcgccagatt acgtaccgta tcgacaaaac
     3661 catgttcggc agcgccaatc atcaaataac gcccacggtc gctggcttca tcaagacgca
     3721 taatgcgttg cggagcaatt ttaattcgcg cgagcagttc acccagcgct tcacggcgac
     3781 cttcactttc taccgagaac accaccggac cgtcgaaaga ctcgaggaac ttacgcagcg
     3841 catccagcgg tgctttttgt tgtgcctgaa cggccaggtc tggcagtttc tggaaaccta
     3901 aattggcatt cgcggctttt gtcggtaaat gttcagtttt tagctgcaca cgcggccagt
     3961 ttttcagctc tgaaaagagc tcgtctaccc gcagccagag cgattgtggt ggcaacagtg
     4021 ggcgcatcgg atcgacgccg cgattttcaa aacgcgccag cgtgtcagcc tggaaacgtt
     4081 cggcactgtt ttccagatcg ccagtattca ccaacagcgt attggcaggg aaataactga
     4141 acagtggcgg cagcggttcg ctgaagaata gcggctgcca gtactcgatc ccggcaggta
     4201 atgtgccttt actcacttgc tgataaatat gttccggatc gcgcttcact tcgaaggtat
     4261 cgcgccactg gctgcgaaac agttcaattg ccgctttatc ggtcggaaat tcgtgcgcgg
     4321 gcagcagatt gatctcttct acttcctcca gcgtgcgctg gctatcgacg tcaaacactc
     4381 gcaggctgtc gatttcatca tcaaagaaat caagacgata aggcagctca ctgcccatcg
     4441 ggaagagatc cagcaacgcg ccgcgcgtgg cgtattcgcc gtgctccatt acctggtcaa
     4501 catggcgata accggcgctg tccagttggg ttcgtaatgc atctcgtgac aggcgctgac
     4561 cttttttcat caccagcgca tgaccgtgga gaaaactgtg cgggcaaacg cgctgcataa
     4621 gcgtattcac cggaacaatc agtacgccac gctgcatggt cggtagctgg taaagggtgg
     4681 aaaggcgcga ggagataatg tcctgatgag gcgaaaaact gtcgtaggga agggtttccc
     4741 agtccgccag attcatcacc atctgatcgg taaactggct gatttcatca tgcaaacgca
     4801 gagcattttg catatctggt gcaataagta ccaccggacc ggcgtgacgt tcggcaattt
     4861 ccgctaccag cgtcgcgcag gcagcgccgg ttaactcgcc cagcagacgc tgctcacccg
     4921 ctttgacggg cagcgtataa cgatattgtt caggcataac ggttgtcaga ttctcgttag
     4981 gatatgcctc aacatatggg ggcatatctc tgataagcaa tgctgttcat tatccgtaat
     5041 cgacatggtt tagcaaattg aatcgccccg gctggggcga tggcttagcg ggagtaactg
     5101 acggcagcgg gcagtgaagg cggggagaac aacagatcgc tgaatgcacg ctgtgaaagt
     5161 tttcttacca gcaagcccgc aacagtacag atgaacaaac tgacaaaggg ataaaccatc
     5221 aggagtgtca gttcgacttg cggcgaccag cgtgccgcgt tcattggcgc aagcagagtt
     5281 aagctgaata tctctaccag aatgcgatgg gtggtgtaga tagcaatggt gttggtgcca
     5341 atcacattca gcaggctggt ggagcgcata ccgaaacgtt gctcgtattg ataaaacagc
     5401 ttcatgatca ccacaatcga taccagcgac aacagcagcg agatagtaaa caaccaggca
     5461 ccgaccgcca gaacggtcag caaagaagtc atcagcagat ggcggcgcaa cggcacctct
     5521 ttaacgtagg tcattaatgt tgcgccaaac catgcgccaa ggctgtaata aggcaaattg
     5581 cggatcacac tgttcattcc ccaccacggc gtgggaacga aattaaccgc cacactcatc
     5641 agtacaaaca aggcgaatag tggcagcgcc agacggctaa aaattttaca taccacgaaa
     5701 taaacaatta acgcatacag ataccacaaa ctggtgctgg cggtgatcat cccgtgcagg
     5761 aactcaccgg tggaatcggc atacgcggca ttggaggcat tgcttaaatc gcgctcaggt
     5821 gccagccact ggttcagcgc acttaacgcc agccactgca ccacgcccca aagcgccagc
     5881 acccagaaga tgttccagat ccgtttatcg agacaatttc cccacggtac gctgtcgata
     5941 taacggcgaa tcaaatagcc ggatataaag aaaaaaaccg gcatacgaaa gggtgcaagg
     6001 taaagattga aatagatcca gcatttgctc aggacttccg ataacggatg ctgaaaagtg
     6061 gtgagatgcg gataaaaggt aatgaccgag tgataaatca ccaccagaca aatacataac
     6121 cctttgatct ggttaatcca tagctctttt tgtttcatca ggacaacacg ctctttttat
     6181 ttaacatgct gttgatcctg aggggccagt cgcgtggtgt aatgcgtatc tgtctgtatt
     6241 tggttatttt tcagataaag atcaggctac aaggccgatg agaaaatgag gccctttagc
     6301 cgattgattt gctggcactt ttcagattag ccctgacgat cacttacagt tcagacgttt
     6361 acccatcccg ctttcgctta tatactcgtg tctttgctaa cagcaaccag acggatttca
     6421 tgtaccaacc tgtcgctcta tttattggcc tgcgttacat gcgtgggcgt gcagcggatc
     6481 gcttcggtcg tttcgtctcc tggctttcta ccatcggcat taccctcggg gtgatggcgc
     6541 tggtcacagt attgtcagtg atgaacggct ttgagcgcga gctacaaaac aacatccttg
     6601 gcctgatgcc acaggcaatt ctctcttctg agcatggctc tcttaacccg cagcaactcc
     6661 cggaaacggc agtcaaactg gacggcgtta atcgcgtcgc acctattact accggtgatg
     6721 tggtactgca aagcgcgcgc agcgtggcgg tcggggtgat gctgggtatc gacccggcac
     6781 aaaaagatcc actaacgccg tatctggtca atgtgaaaca aactgacctc gagccgggga
     6841 aatataatgt catcctcggt gaacagctgg cctcacagtt gggcgtcaat cgcggtgatc
     6901 aaatccgcgt gatggtgcca tctgccagcc agttcacgcc gatggggcgt attccaagcc
     6961 agcgcctgtt caatgtgatt ggcactttcg ccgccaacag tgaagtcgat ggctatgaaa
     7021 tgctggtgaa tattgaggat gcctcgcgtc tgatgcgtta tccggcaggc aatattaccg
     7081 gctggcgttt gtggctggat gagccgctga aagtcgactc attaagtcaa caaaaactgc
     7141 ctgaaggcag caaatggcag gactggcgtg accgtaaagg cgagctgttc caggccgtac
     7201 gcatggaaaa aaatatgatg ggcttgctgc tgagcctgat tgttgccgta gcggcgttta
     7261 acattattac ctcgctaggg ctaatggtga tggagaagca gggcgaagtc gcgatcctgc
     7321 aaacgcaagg cttaactccg cgacaaatca tgatggtctt tatggtgcaa ggggccagcg
     7381 ccgggattat cggtgcgatc ctcggagcgg cgcttggcgc actgcttgcc agccagttaa
     7441 ataatctgat gccgataatc ggcgtcctgc ttgatggcgc ggcgctgccg gtcgctatcg
     7501 aacctttaca ggtcattgtt attgcgctgg tggcgatggc tatcgcgctg ctgtctacgc
     7561 tttacccttc atggcgcgct gccgccacac aacccgctga ggctttacgt tatgaataag
     7621 atcctgttgc aatgcgacaa cctgtgcaaa cgctatcagg aaggcagtgt gcaaaccgat
     7681 gtgctgcaca atgtcagttt cagcgtgggc gaaggtgaaa tgatggcaat tgtcggtagc
     7741 tctggttccg gtaaaagtac cttgctgcac ctgttaggcg ggctggatac gccaacctcc
     7801 ggcgatgtga tctttaacgg tcagccgatg agcaaactgt cgtcggcggc gaaagcagaa
     7861 ctgcgcaacc agaagctggg ctttatttat cagtttcacc acctgctgcc ggattttact
     7921 gccctggaaa acgtggctat gccgctgctg attggcaaga aaaagcccgc tgaaatcaac
     7981 agccgtgcgc tggagatgtt aaaagcggtg gggctggagc atcgtgcgaa tcaccgccca
     8041 tctgaacttt ctggcggcga acgccagcgc gtggcgattg cccgtgcgct ggtcaataac
     8101 ccgcggctgg tgctggcgga tgaacctact ggtaacctcg atgcacgtaa cgccgacagc
     8161 atcttccagt tgcttggaga attgaatcgc ttgcagggca ccgccttcct ggtggttact
     8221 cacgacctgc aactggcaaa acgtatgagc cgccaactgg agatgcgtga tggtcgtctg
     8281 acggcggaac tgagcctgat gggggcggag taatggcgat gcctttatcg ttattgattg
     8341 gcctgcgttt tagccgcgga cggcggcgcg gcggcatggt gtcgctgatc tccgtcattt
     8401 ctaccattgg cattgccctt ggcgtggcgg tattgatcgt cggcttaagc gcgatgaacg
     8461 gctttgaacg cgaactgaat aaccgcattc tggcggtggt gccgcatggc gaaatagagg
     8521 cggtggatca accgtggact aactggcagg aagccctgga taacgtgcaa aaagtgccag
     8581 gtattgccgc cgctgcgccg tatatcaatt tcaccggact ggtagaaagt ggtgccaatc
     8641 tgcgcgcaat ccaggtgaag ggcgttaacc cgcaacagga acagcgtctg agcgcattac
     8701 cctcgtttgt tcagggggat gcctggcgca attttaaagc gggcgaacag caaattatca
     8761 tcggcaaagg cgtggcggat gcgctgaaag tgaagcaggg cgattgggtg tcgattatga
     8821 tccccaactc gaatcccgag cataaactga tgcagccaaa acgtgtgcgt ttgcacattg
     8881 ccggtatttt gcagttgagt ggtcaactcg atcacagttt tgccatgatc ccgctggcgg
     8941 atgcccagca atatcttgat atgggttcca gcgtgtcagg tattgccctt aaaatgacgg
     9001 atgttttcaa cgccaataag ctggtacgcg atgcgggtga agtgaccaac agctatgttt
     9061 atattaaaag ctggattggt acttacggct atatgtatcg cgatatccag atgatccgcg
     9121 ccattatgta tctggcgatg gtactggtga ttggcgtggc ctgtttcaac atcgtctcca
     9181 ccttagtgat ggcggtgaaa gacaagagtg gcgatatcgc agtattaaga acgctggggg
     9241 cgaaagatgg tttaattcgc gccatctttg tctggtatgg attgctggca gggctgttcg
     9301 gtagcctgtg tggtgtgatt atcggcgtag ttgtttcact gcaacttacc ccgattattg
     9361 agtggattga aaagttgatc ggtcatcagt tcctctccag cgatatctat tttattgact
     9421 tcctgccatc ggaattgcac tggctggacg tcttctacgt actggtcaca gcattgttgc
     9481 tgagtctttt ggcaagttgg tatccggcgc gacgcgccag taatattgac cctgcgcgag
     9541 tccttagcgg ccagtaaagg cagtacatta aaacaaggag cggcaatgta ttacgggttt
     9601 gatattggtg gaacaaaaat tgcgcttggc gtgtttgata gcggtcggca gttgcagtgg
     9661 gaaaagcggg tgccgacacc gcgtgacagc tatgacgcat ttttagatgc agtgtgcgag
     9721 ctggtagccg aagctgaccg gcgttttggc tgtaaaggtt ctgtcggcat cggtattccg
     9781 ggaatgccgg aaacagaaga tggtacgctg tatgccgcca atgtccctgc tgccagcggt
     9841 aaaccgctgc gtgccgacct gagcgcacgt cttgatcgcg atgtacgcct tgataacgat
     9901 gccaactgtt ttgccctttc tgaagcatgg gatgatgaat ttactcaata tccactggtg
     9961 atggggttga ttctcggcac cggcgttggc ggcgggctga ttttcaacgg taaacccatt
    10021 accggtaaaa gctatattac cggcgagttt ggccatatgc gtctgccggt tgatgcgtta
    10081 accatgatgg ggctggattt cccgttacgc cgctgcggct gtggtcagca tggctgcatt
    10141 gaaaattatc tgtctggtcg cggttttgcg tggctgtatc aacactatta tcatcaaccg
    10201 ttgcaggctc ccgaaattat tgcgctttat gatcaaggtg atgagcaggc aagggcgcac
    10261 gttgagcgtt atctggattt attagcggtt tgtctgggaa atatcctgac cattgttgac
    10321 cctgacctgg tcgtcattgg tggtggctta tcgaatttcc cggcaatcac aacgcaactg
    10381 gcggacaggc tgcctcgtca tctcttacct gtagctcgtg ttccgcgcat tgaacgcgcg
    10441 cgccacggtg atgcgggagg aatgcgtggt gcggccttcc tacatctaac cgattaaaca
    10501 acagaggttg ctatgctgtc gcgtcggggt catcggttaa gtcgttttcg taaaaataaa
    10561 cgccgtctgc gcgagcgttt gcgtcagcgt atttttttca gagataaagt ggtgccggaa
    10621 gcaatggaaa aaccaagagt actcgtactg acaggggcag ggatttctgc ggaatcaggt
    10681 attcgtacct ttcgcgccgc agatggcctg tgggaagaac atcgggttga agatgtggca
    10741 acgccggaag gtttcgatcg cgatcctgaa ctggtgcaaa cgttttataa cgcccgtcgt
    10801 cgacagctgc agcagccaga aattcagcct aacgccgcgc atcttgcgct ggcgaaactg
    10861 caagacgccc tcggcgatcg ctttttgctg gtgacgcaga atatcgacaa cctgcatgaa
    10921 cgcgcaggta ataccaatgt cattcatatg catggggaac tgctgaaagt tcgctgttcg
    10981 caaagtggtc aggttctcga ctggaccgga gatgttaccc cagaagataa atgccattgt
    11041 tgccagttcc cggcaccctt gcgcccgcac gtagtgtggt ttggcgaaat gccactcggc
    11101 atggatgaaa tttatatggc gttgtcgatg gccgatattt tcattgctat tggtacttcg
    11161 gggcatgttt atccggcggc tgggtttgtt cacgaagcga aactgcatgg cgcgcacacc
    11221 gtggaactga atcttgaacc gagtcaggtt ggtaatgaat ttgccgagaa atattacggt
    11281 ccggcaagcc aggtggtgcc agagtttgtt gaaaagttgc tgaagggatt ataaagcggg
    11341 aaaaattgcc tgatgcgcta cgcttgtcag gcctgtgaag gggcgcaatt aattgaattt
    11401 gcgggttttg tgggccggat aaggcgttcg cgccgcatcc ggcaacttta ctgcctcttt
    11461 taccgtttac tcgtccatag taacgcggca ataaacgtac agacaccaat cagccctgcc
    11521 cagagcggcg atattctggt aatcgcaaag ctgttatcga tatacaaaca taaagcctga
    11581 tagcccatat tggcaacagc catagccact gcaaatttta acgcataata aaggcgcgtt
    11641 gtgtagttaa aaaatgcagc aaagacgatg aatgcaactg cccaggcaaa aaggcctaat
    11701 gtcgtggtac tgccgggaag atcattctcc tgctgttgtc cgggatgcgg ataatatgtc
    11761 agtggcggcg tcggttgttt taatatctgt gcttgtaaaa cagccaatgc attattgatt
    11821 ccctgatacc agagatctgc atgtaaataa gccagagtgt gattttctac cagaaggccg
    11881 agatgtttat cgtccatgct gctttcaacc tggccgttgg ttatcataat taaccgtggc
    11941 tcgcgggtag taaaaagtaa cagaacactt ccttttacat tactcattga agatgccgca
    12001 tgcgccagca tcgctttgcc attttgcctg aagcttgctt cattaacgcc aggtacaacc
    12061 acgataaacg gcgcaatccc ggtttgttga cgaaaaagtt cggctttacg cgcgatctct
    12121 tcggcatccg gtacagtacc ggtaacatcc gtaaaaagag tcttcattat tggaatcaat
    12181 ggcttactgg ttaccgtcag aggcaggagt atcagtaaac tgagcaatat tatgattttt
    12241 ttcatgggcg ggacttccgt ttcctgcgtg tctttttaga gtagggcata ctgattaata
    12301 aaccgacgga gataaaaata acgcccataa tgccgataaa cccccattta aaaaaattaa
    12361 cccacgggtt atttatctca gccttttcag gatgtcctgg agaataataa acattaactt
    12421 tatcgccttc actgtcttca taaaaatcgc tgccaatact tgaattgaaa attagcgtgt
    12481 aatctggcgt cggacgaaag gcaacgacgg gataccacga accattttta cccgtgcgat
    12541 gagaatgact ggagtgccag acggtatcaa caatgacgcc ttcagtatga atcgcgtctt
    12601 tgctataggt gaactggtta taaaagataa caacagaaat gaggatcagt accagaccga
    12661 taccgaagaa tatacgaaag aaaactctac tgtcctggct cattagccac atccttgcta
    12721 aataaaaaac gggcggtaat accaccgccc gcttgctgaa ttaacgtcct gctttcagct
    12781 tctgataata ctcttcataa atgctgctgg ctgcgccaac gtcattctgc cattcaccat
    12841 ttttaatggt ttcagcatcc gggtagagcg ttttatcgtt cgccacttcc ggacttaaca
    12901 gcttacgcgc cgcaaggttt ggcgttggat aaccgatagt ttcagcaacc tgttttgcca
    12961 catccgggcg cagcaggaag ttgatcaatt tcagtgcgcc ttctttgttt ttggcatttg
    13021 ccgggatcgc caggctgtcc atccagaaaa tgccgccttc tttcggccac accacatcaa
    13081 ttggcgtacc cgcctggcgt gcaacgaaag cagaaccgtt ccagatcatg ccgaggttaa
    13141 cttcgccttc catatacggg ttagcggggt tatcggagtt aaacgccgcc acgtttggca
    13201 tcagtttttt cagctcgttg tatgcagctt caatctcttt cggatcggtg gtgttaccgg
    13261 agtagcccag cttacgcagc gccatctgga acacttcacg ggcatcgtcg gtcagcagca
    13321 ggctgccttt gtactcaggc ttccacagat cggcccagct ggtgacagat ttcggatcca
    13381 ccgcatcacc gttaacgcca atcgccgttg caccccagat atacggaatg gaatagtcgt
    13441 tattcgggtc aaacggcttg ttgagcatgt ctggatcgag gttgctgaaa ttgcttaact
    13501 tcgacttgtc gatcttctgg atcatccctt ctttacgcat tttatcgacg taataggttg
    13561 aaggaaccac caggtcatag gcaccgtctt tgtacgtttt cagcttcgcg tacatggttt
    13621 cgttcgactc gtaagtcgaa tagataacct taataccggt ttctttggtg aactgttcaa
    13681 gcagtcctgg cggcacgtac tcggtccagt tgtagaaata cagcgtgttg ttgtcatcgg
    13741 cgtgagcggc gctcatgccc agtgccagag cacccgccgc gagcaggtgg cgtgaccatt
    13801 ttttcattta acgtcccctg tgttaccttt cgttttatca cgagcaataa gctggctggc
    13861 aattaccatc accagcgaca gcaccagtaa tatggttgcc agcgcgttaa cttccggcga
    13921 tacgccgact ttgaccatcg aataaatttt taacggcaga atttcataac tcggcccggt
    13981 gacaaacgaa gaaaccacca cgtcgtccat cgacagggta aagcttaaca cccagcccgc
    14041 cgctaccgct ggcattgcca gaggcagaat gatttttcgc agaatggtaa attcgctggc
    14101 accgagatct ttcgccgctt ccagcattcg cacgtcaaaa cctttcaggc gcgaatacac
    14161 tgtcaccacc acaaatggca ggcagaaggt gatatgcgag aacagcagcg accagaagcc
    14221 aagctgaata cccagcagca taaacagcac cagcagcgaa atcgccatca cgatatctgg
    14281 tgacatcatc accacaaaca gcattccgct aacgaacggc ttaccacgaa agcgataacg
    14341 gtacagcgca actgctgtca gtgaaccgat aagcgtagca aacgtcgccg aaaacaccgc
    14401 cattgttagt gaatgctgcg ctgcctgtaa caggctgtcg ttgttcatca gcaggctata
    14461 ccatttggtg gtaaaaccct gccagttgat gccaaagcgc gagctgttaa aggagttcac
    14521 aatcaaaata atgattggga tatacaggta cgcgtagata gcggtcataa aaccgccgcg
    14581 aagcagtcga ccgatcattc gagttccacc ttcttattca gcagacgaga agcgcgccag
    14641 taaaccagca acatcaggcc cattacgata gtcagcgtaa tgctggtggc tgcaccaaac
    14701 ggccagtcac gaatattgag gaactgtacc ttgatgacgt taccgatcag caggtttttc
    14761 gcaccgccca tcaggtcgga cacatagaac aggcccatcg ctggcagcat caccagcaga
    14821 catccggcaa taattcccgg catcgttagt gggataatga tgcggataaa ggtctgcaac
    14881 ttgctggcac cgagatcgcg cgccgcctca agcaacggct tatccagttt ttcgatactg
    14941 gagtacagcg gcatcaccat aaacggcagc agaatgtaaa ccagaccgat aatcaccgca
    15001 ctaggcgtga acatgatgcg aattggtgta tcgataacgc ccagccagag caaaaactcg
    15061 ttgagatagc ctttggtgct gaggaaaatt ttcagcccgt aaatacgaat caatgagttg
    15121 gtccagaacg gaacaatcag cagaaacagc agcagcggac gcaccttgtg tggcagcttc
    15181 gccagaaacc aggcaaaagg atagcccagc accaggcagg cgagggtggc gatcagcgcc
    15241 atattcagcg agtgcaatag cacttcaaaa tagagcggat cgagcagacg cgtgtagtta
    15301 tccagcgtaa agaccatttt gacgaaactg gcgtcgtcgc gggtcaaaaa gctggtgcca
    15361 atgatcatca ggttgggcag aaagacaaac aacacaagcc aaccgacaat agtgacaatc
    15421 actacattct ggaacttact tgtgttcttc atcagccagt acgacctccc agctttctac
    15481 ccaattaatg gccatttttt ggtcgagaga gtggtcaaag tcaggatcgt cttcattgaa
    15541 gaattcgctg accatcacca tcttgccatt ttccagctca acaaccgact ccagcgtcat
    15601 gcctttgtag ttacgctcgc gaacgtaacc aatcagccct tcggcgtggt tgtcgtcgtt
    15661 aatctcttca acacgtaagt cttccgggcg cagcagaaca tgcagttttt gccccggttc
    15721 aacggtgaag ttaacgtaga tattacattc gcggccttca acgttggcgc gtacgcgctg
    15781 ctcgtcgaga cgttcgatga cagtggcgtt aaacatattg atttcgccaa taaacccggc
    15841 aacaaacagg tttttcggct cttcgtagat ttcacgcggc gtgccgtctt gctcaatgcg
    15901 accatcgcgc atcaccacga tcctgtccga catggtaagt gcttcttcct ggtcgtgagt
    15961 tacgaacacg aatgtaatac caagcttacg ctgtaacgct ttcagctcgt tctgcatttg
    16021 cttgcgcagt ttgtaatcca gcgctgagag cgactcatcc agcaacaaca gacgaggctt
    16081 gttaaccacc gcgcgggcaa tggcgacgcg ttgctgttga ccaccagaga gctgatgcgg
    16141 tttgcgttga gcgaaggttt ccagctgcac catccgcagg gcttccatca cgcggggcgt
    16201 aatttcatca gcgggggttt tttgcatgcg caacccaaag gccacatttt cgaacacggt
    16261 catgtggggg aaaagtgcgt agctttggaa aacagtgttc acgtagcggt tttccgccgg
    16321 aacgtgggtg atgtcctcgt tatccaacat gatgcgtccg gaatcaacag tttccagacc
    16381 tgcaatcagg cgaagaacgg ttgttttacc gcagccagaa gggccaagca gcgtgaggaa
    16441 ctcgccattg ttgatagtca gatccagctg gggaatgacc tctttaccat caaagcattt
    16501 gcgaattccc gccaattgca ccagcggtga aagcgaactc ggttgtttat tcaatttttt
    16561 actctgtccc atgtaaacgc aacggatggc ttaccgatgc ggggtttgtg gttaaccacc
    16621 ttggtgactc ttaatgaggg cggtaattct acggcaaacc gcttgaatcg ccaatctttg
    16681 ttgtgaatta ctggcttagc tttatattca ttaaggtaat gctgataaat attcccgctt
    16741 gcagggctaa aagtgacctg acgcaatatt tgtcttttct tgcttcttaa taatgttgtc
    16801 acaaaaagtg agggtgacta catggataaa ctacttgagc gatttttgaa ctacgtgtct
    16861 ctggataccc aatcaaaggc aggggtgaga caggttccca gcacggaagg ccaatggaag
    16921 ttattgcatc tgctgaaaga gcagctcgaa gagatggggc ttatcaatgt gaccttaagt
    16981 gagaagggca ctttgatggc gacgttaccg gctaacgtcc ctggcgatat cccggcgatt
    17041 ggctttattt ctcatgtgga tacctcaccg gattgcagcg gcaaaaatgt gaatccgcaa
    17101 attgttgaaa actatcgcgg tggcgatatt gcgctgggta tcggcgatga agttttatca
    17161 ccggttatgt tcccggtgct gcatcagcta ctgggtcaga cgctgattac caccgatggt
    17221 aaaaccttgt taggtgccga tgacaaagca ggtattgcag aaatcatgac cgcgctggcg
    17281 gtattgcaac aaaaaaacat tccgcatggt gatattcgcg tcgcctttac cccggatgaa
    17341 gaagtgggca aaggggcgaa gcattttgat gttgacgcct tcgatgcccg ctgggcttac
    17401 accgttgatg gtggtggcgt aggcgaactg gagtttgaaa acttcaacgc cgcgtcggtc
    17461 aatatcaaaa ttgtcggtaa caatgttcac ccgggcacgg cgaaaggagt aatggtaaat
    17521 gcgctgtcgc tggcggcacg tattcatgcg gaagttccgg cggatgaaag cccggaaatg
    17581 acagaaggct atgaaggttt ctatcacctg gcgagcatga aaggcaccgt tgaacgggcc
    17641 gatatgcact acatcatccg tgatttcgac cgtaaacagt ttgaagcgcg taaacgtaaa
    17701 atgatggaga tcgccaaaaa agtgggcaaa gggttacatc ctgattgcta cattgaactg
    17761 gtgattgaag acagttacta caatatgcgc gagaaagtgg ttgagcatcc gcatattctc
    17821 gatatcgccc agcaggcgat gcgtgactgc gatattgaac cggaactgaa accgatccgc
    17881 ggcggtaccg acggcgcgca gttgtcgttt atgggattac cgtgcccgaa cctgttcact
    17941 ggcggttaca actatcatgg taagcatgag tttgtgactc tggaaggtat ggaaaaagcg
    18001 gtgcaggtga tcgtccgtat tgccgagttg acggcgcaac ggaagtcgtg attgcccgat
    18061 gcgtatgtgg actgccaatt accgggatga aacctttata ttgacactgg tccacatact
    18121 tatcgtgcct acaggcgttc agtgcatgta ggcacgataa ggcgcaatca gcgccgcatc
    18181 cggcttaaat ccaaacttac ccttcgaaga accagtatcc gctattgacc agcgccgcga
    18241 gcatcgcgag gaatgacgga tcttccagcg catcgccaaa attctccgcg gtcagcgcaa
    18301 tgttgctggc gagcgcatcc agtgccggac ggtgcgggga atcgatcttc tcaccattgg
    18361 catacacgtc gtcgccaatg cgcaatacgc gcagaccccc caggcgcacc agcacatcac
    18421 cttgtttcag cgcatcgtag atttcatccg gctgataagg cggctctggc ggcgcgatat
    18481 ccagttcatg acgtgactgg gatataaact cgccaaacca ttgcttaaaa tgttccggct
    18541 ggttgatcaa ttcgagcatc atctcacgca gtttatccat ctcttgcggc agaacgtccg
    18601 caggatgagc gcgaggtgga acatccggat cgctgtagta gttgccgccc agttcacgtt
    18661 gcagcacata atcggcaaat ccactaatca gttcccgcgt atttggcgcg cgaaaaccca
    18721 cggaatagtt catcgcattt tccagcgcgt agccttcatg cgggaatcct ggcggaatat
    18781 aaagaatatc accaggctcc agctcttcat cgatgatggc ttcgaacgga tcgacctgta
    18841 acagatccgg atgtgggcag tgctgtttca tttgcagctt ttcgcccact cgccagcgac
    18901 gacgtccggt accctgaatg ataaacacgt cgtactgatc gagatgcggg ccgacgccgc
    18961 cgccaggtac agaaaaagag atcatcaggt catcaatacg ccagtccggc agttcacgga
    19021 acggtcgcat cagcgcggcg gtcggctcat gccagtggtt cactgcttgc actaacaatg
    19081 accagctagt ttcaccgaga tgatcgtagc tttcgaatgg accgtggctg acctgccatt
    19141 tgccatcctg atgactgacc agtcgactgt cgacttcgct ttccatcgcc agacccgcca
    19201 actcgtctgg agagatcggg tcaataaaat tattaaagcc gcgttttaac accaccgggc
    19261 gtttctgcca gtgacgttca agaaaatcgg gccagttaag agtgagttgg tattccatgt
    19321 taagcatcca caggctggta tctgcaaccg attataacgg atgcttaacg taatgcgtga
    19381 agtatggaca tatttattca tctttcggcg cagaatgctg gcgaccaaaa atcacctcca
    19441 tccgcgcacc gcccagcatg ctctctccgg cgacgatttt accctcatat tgctcggtga
    19501 tttcgcgggc taccgccagc cccacacctt gcccagggcg taaagtatca acccgttgac
    19561 cacggtcgaa aatgacctct cgcttgctta atggaatacc ggggccatca tcctcgacca
    19621 caatatagag atgctcgtcg gtttgccttg cagaaatttc gacaaactcg aggcaatatt
    19681 tacaggcatt atccagcaca ttgcccatca cctcgacaaa atcgttctgc tcaccgacaa
    19741 agctgatctc tggcgaaata tcgagagaga tattgacccc tttgcgttga tacactttgt
    19801 tcagcgccga ggtgagattg tccagcagtg gagcgaccgg atgcagctcg cggctgagca
    19861 atgtcccgcc gcgcatactg gcacgatgca ggtagtagcc aatttgctgt gaaatgcggc
    19921 tgatttgctc cagcattacc ggttcagcat cactgacgct catcttttca ctacgcagag
    19981 aacgcagcgt actttgcagc accgccagtg gcgttttcag actatgggtc aggtcggtga
    20041 gcgtcgtacg atatttgtcg taacgttcgc gttcactttt taacaatcgg ttcaggtttc
    20101 gtaccagact ggtcagttct cgcgttgtgg ctggattgag caattcgcgg ttatgttctt
    20161 ccagttcgcg gacttctttt gccagggctt cgatggggcg taaactccac caggcggcga
    20221 cccacagcag cgggatcact aacagcagat tggctgagag cacatagata aaccagctcc
    20281 agaccatata ggaacttttt agctccaccg gaatggtatc caccaccaca atggtcaact
    20341 ttggcatccg cgatgttgcc gggtagacgt ttaccgccac cgagtgggtc atctccgcgt
    20401 cgtcatcatc ttcccgcact tcctgcaact gttgctgtat cgaatgatct ccactcagca
    20461 agaggctggt atcgttaaca tccgcttcaa tttcatgaaa accattcgat ttcagccagt
    20521 caggctggat catcttcatc agccagggca cgtcacgttg cgcccataaa agctgcccgt
    20581 tctcatcata aattagtgtc atcgtggggc tttgcttgtc gatattttcg ggtaactcga
    20641 catgcaactt attgttttcc cacttcgcaa gggtatagaa cagattgctc tcgccacgta
    20701 acagccgaaa cgtggtttta tcgaaactga cgctataacc gatcagcgcg accattccgt
    20761 aggcaagcga aagcaccagt actaccgctg ccgttgccaa cagaaaacgt acccgcagcg
    20821 agagcgggaa aaaaagacgc agtaattttt tcatcagcgc aattcgaaca gatagccctg
    20881 gccgcgaacg gtggtaatca cttcttgcgg atattgtgcc tgaatttttt tgcgcagacg
    20941 tcccatcagt acatcaatgg tatggctttc ccgcagctcc gcatccggat agagttggag
    21001 cattaacgaa tctttgctca ccactttgcc attattgcgt atcaacgttt ccataatggt
    21061 gtattcgaac gcggtcagtt tgatcacttc gtcattaata gataattcac ggcgagagag
    21121 atcaacctga aacggtggta gcgaaatgac ctgtgaagcc agaccgctat tacgccgcat
    21181 taatgcctgc attcgcgcca tcacctcttc aatatgaaac ggtttagtca cgtaatcatc
    21241 agcgccagca cttaatactt cgactttgtc ctgccagctt tcacgggcgg ttaataccag
    21301 aatcggcagt gaaacatcat tgctacgcca gcggcgaatc agtgacagac cgtcctcgtc
    21361 aggcaaaccg agatcgacaa tcgcaatatc cggtaaatgt tcattgagat aataatcggc
    21421 ttctttggca tcttctgcgt catcgacctg atgaccagca tcctgaatct gaactttaag
    21481 atggtgacgt aacaacgcat tgtcttcaac aaccagtacg cgcattttta tttctccctg
    21541 tcttaattat taaaatagtg taacgcgatt atgtggttat gggggtaaac attaaataaa
    21601 ccagcgggaa ggggaggtaa agtgaaaaaa caaaaagtgg ataatcttaa taagcaggcc
    21661 ggacagcatc gccatccggc actgatttga ggcttatttc agctcatcaa ccatggtgat
    21721 ggcgcgacca atatagttag ccggcgtcat cgctttcagg cgggctttct cttcttctgg
    21781 caacgccaga ccgtcgataa actgcttcat gccttcggcg tcaacgcgct taccgcgagt
    21841 cagctctttc agcttctcgt acggtttttc gatgccatag cgacgcataa ctgtctggat
    21901 tggctcagcc agcacttccc agttgtgatc cagttcatcc agcagatggt cacggttcac
    21961 ttccagtttg ctcacccctt tcagggtgga ttgatacgca atcagcgcat aaccgatacc
    22021 cacgccgagg ttacgcagca cggtggagtc ggtcaggtca cgctgccagc gggaaaccgg
    22081 cagtttgctt gccagatgct gcaataccgc gttggaaagg cccagattcc cttcggagtt
    22141 ttcgaagtcg atcgggttaa ctttatgcgg catggtggaa gaaccaatct caccagcaat
    22201 ggttttctgt ttgaagtggt taagggcgat ataaccccag acgtcacggt caaagtcgat
    22261 cagaatggtg ttgaagcgcg caacgcaatc aaacagctcg gcaatgtagt cgtgcggttc
    22321 gatctgggta gtgtacgggt tccactgaat acccagcgag gtgacgaact cttcgctgaa
    22381 ctgatgccag tcaacttccg ggtaagcggc gatgtgggcg ttatagttac cgactgcgcc
    22441 gttgatttta ccgaggatct ccacctgatt aagctggcgg tactggcgct ccatacggta
    22501 ggcgacgtta gccatctctt taccgatggt tgacggcgtg gctggctgac cgtgggtacg
    22561 ggacagcagc gggatatcgc gatactgaac ggcgagatct ttaatgccat caatcagttg
    22621 acgccagtac ggcaggatca cttcatcacg cgcggttttc agcatcaacg cgtgggagag
    22681 gttattgata tcttccgaag tacaggcaaa gtggatgaat tcagaaaccg catgcagttc
    22741 cgggatctcc gccacttttt ctttcaggaa atactcaacc gctttaacgt cgtggttagt
    22801 ggtacgctcg atggttttga tgcgtgctgc atcttcttcg ctgaaactgg cgacaattgc
    22861 atcaaggtaa ccgattgcgt cggcagcaaa agcaggaact tccttgatcg ctgcgtgcgc
    22921 ggccagtttt tgcagccaac gtacttcaac ttgtacacgg aatttcagca aaccatattc
    22981 gctgaaaatc ccgcgcagcg cgctgacttt atcgccgtag cgtccatcga caggggaaac
    23041 ggcggtcagt gaggataatt ccatagatca caactccggg gttaaatgag caagaatttg
    23101 ttttgcctga gtggtcaggc gattacgaga aaacatcagt tgcagacgtc cgccgccgac
    23161 ctggtgccag agcacggcgg cgcgaatgcc tgccagcagg gtagcgcgaa ctttcgcctg
    23221 cacttgtggg ctttgcagta cagcagggga accggtgacc tgaatgcgcg ggccaagcgg
    23281 gctaatcaca tcaacataga tagcagccat cgcgctcatc agcgtttcgg actgtaaatc
    23341 gaagtgttcg agctggcgtt gcaggccgtt gatccggttg cccagagtgt cgagcgcacc
    23401 tttcgctgag gagagtttgc gctcaagcac catcaggctg agtgtgtagc gggttaactc
    23461 agcgtttaag ccctggcgac tgctggcatt gagcacgccg agcaaggttt ccagcccgac
    23521 gcgcaggttg gcttcgctac cgccaaaaac cgccagcgtc gagctggggt tcatatcaat
    23581 aatactgttg agtgagacgt gtagcgcatc ggcatcacaa tgcccctggt gagcgagttg
    23641 ttgcaccagg cgtgccgact gacaaatacc ggccagagcg agggtgatgt catagtaatt
    23701 ctttgccacg ttcactgctt ccttgtttta agtaaagaaa ataatcagac cggcagcggc
    23761 agacgctgct caataatacc gccaccgagg cacacttcac cgtcatagaa tacggcagac
    23821 tggcccggcg tcacggcggc aaccggctca tcgaaaatca cttcaatgcg atcatcgtcc
    23881 agcgccttga cggtgcacgg aatgtcggtc tggcgatagc gggtttttac cgtgcaacgc
    23941 atagtgccgg tgaatggttc gcgatcgacc cagtgcaact gctgggcaat cagaccgaca
    24001 gacatcagac gcgggtgttc atggccctgc gcaacaatca gaatgttgtt ttcgacgtct
    24061 ttatcgacca cataccacgg ttcttcggtg ccttctttgg tgccaccaat acccagacct
    24121 ttacgctgac cgagggtgtg atacatcagc ccctggtgtt caccaatttc atcgccatcg
    24181 acggtaatga ttttgcccgg ttgcgccggg agataacggc ccaggaactc gcggaattta
    24241 cgttcgccaa tgaagcaaat gccggtagag tcttttttct tcgcggtgac cagaccaaga
    24301 tcttcagcaa tcttacgcac ctgcggtttt tccagttcgc cgaccgggaa caggctttgc
    24361 gcaatctgct catggctgag cgtataaagg aagtagctct ggtctttatt gctgtccaga
    24421 ccacgcagca ggcggctctt gccatcgaca tctgcccgac gtacgtaatg accggtagcg
    24481 atataatcgg cacctaaatc ttcggcggca aattcgagga aggctttaaa tttgatctct
    24541 ttgttgcaca gaatatccgg attcggcgtg cgaccggctt tatattcggc aaggaacagt
    24601 tcgaagacgt tgtcccagta ctcggcagca aagttaacgg tgtgcagttc aatgccgagc
    24661 ttgtcgcaga cagcctgggc atcagccaga tccgctgccg ctgtgcaata ttcctcaccg
    24721 tcgtcttctt cccagttctt cataaacagg ccttcgacct gatatccctg ttgttgcaac
    24781 agccaggcag aaacggagga atcgacaccg ccggacatgc cgacgattac tttttttgcg
    24841 gtttcagaca ttggatcact cacgacattg aacttcaagg cggcgtattc tatcacgcag
    24901 ccttttagat gacacccttt gtaaaaggcc agttaaaatc gccaatcatc tccagcggat
    24961 agcgttgccc gctttgataa caacgaatac tttccgccac cagcggcgaa cgcagatttg
    25021 acgcctgtaa aatttcttcg gcgctgaccc aacggcagca gtcgatatcg ctgtcatgag
    25081 gctgggtcgg gcatatttgc tcaagctcaa tagcaaacag gaaacgcaaa aacggcgttt
    25141 tatctggcgc aatccactga tgcatacgaa taaagtgttg cggctgcgcg ctgatgccgg
    25201 tttcttccca cagctcacgc gcagcagctt ccactagcgt ctcatccgct tccagatgcc
    25261 cggcaggttg gttccataac gctttaccat taatcgtctc ttcaacgact aaaaatttgc
    25321 cttctgcgtg caccacgcag gcaacggtaa cgtgcggttt aaacattttg cgtccttaat
    25381 ctgtcgcttc tcgccattcg ccattggcaa gattatccaa agagtaatca cccatcgcat
    25441 agcgaatcag tcgcagcgtg gggaaaccaa catgggcggt catgcggcgc acctggcgat
    25501 ttcgtccttc atataaggtg atcttcagcc agctggtggg aatacttttg cgttcacgaa
    25561 ttggcggatt acggggccat aaccattccg gctcctcaac catctctgca cctgcgggca
    25621 gggtagggcc atcatttaag gttacgccat tgcgcaacgc ttcaagggcg tcttccgtgg
    25681 gaataccttc tacctgtaca taatagattt tcccggtacg tttacccggc tgggttaaac
    25741 gcgcctgcaa cgcgccgtta ttggtcagca ccagtaaccc ttcgctatcg cggtcaaggc
    25801 gacctgctgc ataaacgcct tgaaccggga tgaattcttt taatgttttg cgtccggctt
    25861 catcggtgaa ctgcggaaga acatcgtagg gtttattgaa caggatcaca cgcgtgggct
    25921 ggttttcagg tttacgcctg gtagaacgtt gcgagctgaa tcgcttaacc tggtgatttc
    25981 taaaagaagt tttttgcatg gtattttcag agattatgaa ttgccgcatt atagcctaat
    26041 aacgcgcatc tttcatgacg gcaaacaata gggtagtatt gacaagccaa ttacaaatca
    26101 ttaacaaaaa attgctctaa agcatccgta tcgcaggacg caaacgcata tgcaacgtgg
    26161 tggcagacga gcaaaccagt agcgctcgaa ggagaggtga atggaaagta aagtagttgt
    26221 tccggcacaa ggcaagaaga tcaccctgca aaacagcaaa ctcaacgttc ctgaaaatcc
    26281 gattatccct tacattgaag gtgatggaat cggtgtagat gtaaccccag ccatgctgaa
    26341 agtggtcgac gctgcagtcg agaaagccta taaaggcgag cgtaaaatct cctggatgga
    26401 aatttacacc ggtgaaaaat ccacacaggt ttatggtcag gatgtctggc tgcctgctga
    26461 aacccttgat ctgattcgtg aatatcgcgt tgccattaaa ggcccgctga ccactccggt
    26521 tggtggcggt attcgctctc tgaacgttgc cctgcgccag gaactggatc tctacatctg
    26581 cctgcgtccg gtacgttact accagggcac cccaagcccg gttaaacacc ctgaactgac
    26641 cgatatggtt atcttccgtg aaaactcgga agacatttat gcgggtatcg aatggaaagc
    26701 agactctgcc gacgccgaga aagtgattaa attcctgcgt gaagagatgg gcgtgaagaa
    26761 aattcgtttc ccggaacatt gcggtatcgg tattaagccg tgttctgaag aaggcaccaa
    26821 acgtctggtg cgtgcagcga tcgaatacgc aattgcgaac gatcgtgact ctgtgaccct
    26881 ggtgcacaaa ggcaacatca tgaagttcac cgaaggcgcg tttaaagatt ggggctacca
    26941 gctggcgcgt gaagagtttg gcggtgaact gatcgacggc ggcccgtggc tgaaagttaa
    27001 aaacccgaat accggcaaag agatcgtcat taaagatgtg attgctgatg cattcctgca
    27061 acagatcctg ctgcgtccgg ctgaatatga tgttatcgcc tgtatgaacc tgaacggtga
    27121 ctacatttct gacgctctgg ctgcacaggt cggcggtatc ggtatcgccc caggcgcaaa
    27181 catcggtgac gaatgcgccc tgtttgaagc cacccacggt actgcgccga aatatgccgg
    27241 tcaggacaaa gttaaccctg gctctattat tctctccgct gagatgatgt tacgccatat
    27301 gggctggact gaagccgcag acctgattgt taaaggtatg gaaggcgcaa tcaacgccaa
    27361 gactgtaacc tatgacttcg aacgtctgat ggaaggcgct aaactgctga aatgcagcga
    27421 gtttggtgat gcgatcatca agaacatgta atctctacat gtgttaaata ttgaaacggg
    27481 cgtataacac gcccgtt
//
LOCUS       DAZGHF010000063        26579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGHF010000063 DAZGHF010000000
VERSION     DAZGHF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.063"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..77
                     /locus_tag="ACHK3A_002699"
     CDS             <1..77
                     /locus_tag="ACHK3A_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8740756.1"
                     /translation="GGKQWKGLMTRREIEREICLWGQQ"
     gene            294..476
                     /gene="rzoD"
                     /locus_tag="ACHK3A_002700"
     CDS             294..476
                     /gene="rzoD"
                     /locus_tag="ACHK3A_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO8740757.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(567..860)
                     /gene="iss"
                     /locus_tag="ACHK3A_002701"
     CDS             complement(567..860)
                     /gene="iss"
                     /locus_tag="ACHK3A_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO8740758.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(1221..1415)
                     /gene="ylcI"
                     /locus_tag="ACHK3A_002702"
     CDS             complement(1221..1415)
                     /gene="ylcI"
                     /locus_tag="ACHK3A_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8740759.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            1805..2350
                     /gene="nohD"
                     /locus_tag="ACHK3A_002703"
     CDS             1805..2350
                     /gene="nohD"
                     /locus_tag="ACHK3A_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO8740760.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            2325..4250
                     /locus_tag="ACHK3A_002704"
     CDS             2325..4250
                     /locus_tag="ACHK3A_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO8740761.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            4247..4453
                     /locus_tag="ACHK3A_002705"
     CDS             4247..4453
                     /locus_tag="ACHK3A_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO8740762.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            4450..6051
                     /locus_tag="ACHK3A_002706"
     CDS             4450..6051
                     /locus_tag="ACHK3A_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8740763.1"
                     /translation="MKTSSIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            6032..7351
                     /locus_tag="ACHK3A_002707"
     CDS             6032..7351
                     /locus_tag="ACHK3A_002707"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO8740764.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            7361..7693
                     /locus_tag="ACHK3A_002708"
     CDS             7361..7693
                     /locus_tag="ACHK3A_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO8740765.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            7749..8774
                     /locus_tag="ACHK3A_002709"
     CDS             7749..8774
                     /locus_tag="ACHK3A_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO8740766.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            8816..9211
                     /locus_tag="ACHK3A_002710"
     CDS             8816..9211
                     /locus_tag="ACHK3A_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO8740767.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            9223..9576
                     /locus_tag="ACHK3A_002711"
     CDS             9223..9576
                     /locus_tag="ACHK3A_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO8740768.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9588..10166
                     /locus_tag="ACHK3A_002712"
     CDS             9588..10166
                     /locus_tag="ACHK3A_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8740769.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRISGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            10163..10558
                     /locus_tag="ACHK3A_002713"
     CDS             10163..10558
                     /locus_tag="ACHK3A_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO8740770.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            10566..11306
                     /locus_tag="ACHK3A_002714"
     CDS             10566..11306
                     /locus_tag="ACHK3A_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8740771.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            11322..11744
                     /locus_tag="ACHK3A_002715"
     CDS             11322..11744
                     /locus_tag="ACHK3A_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8740772.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            11726..12160
                     /locus_tag="ACHK3A_002716"
     CDS             11726..12160
                     /locus_tag="ACHK3A_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO8740773.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            12153..14732
                     /locus_tag="ACHK3A_002717"
     CDS             12153..14732
                     /locus_tag="ACHK3A_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8740774.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            14729..15058
                     /locus_tag="ACHK3A_002718"
     CDS             14729..15058
                     /locus_tag="ACHK3A_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8740775.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            15058..15756
                     /locus_tag="ACHK3A_002719"
     CDS             15058..15756
                     /locus_tag="ACHK3A_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8740776.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15762..16505
                     /locus_tag="ACHK3A_002720"
     CDS             15762..16505
                     /locus_tag="ACHK3A_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8740777.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            16502..17074
                     /locus_tag="ACHK3A_002721"
     CDS             16502..17074
                     /locus_tag="ACHK3A_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8740778.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            17134..20631
                     /locus_tag="ACHK3A_002722"
     CDS             17134..20631
                     /locus_tag="ACHK3A_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO8740779.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITW
                     TITSANIDRLRVTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPIWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20702..21301
                     /locus_tag="ACHK3A_002723"
     CDS             20702..21301
                     /locus_tag="ACHK3A_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFO8740780.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            21366..24392
                     /locus_tag="ACHK3A_002724"
     CDS             21366..24392
                     /locus_tag="ACHK3A_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO8740781.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLASGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTTYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYVLMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTH
                     SVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            24389..25291
                     /locus_tag="ACHK3A_002725"
     CDS             24389..25291
                     /locus_tag="ACHK3A_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HFO8740782.1"
                     /translation="MITLILSAPVTEMAEAFKRVFANADNVNIVGKPFETIREFDCMV
                     SAANSFGLMDGGVDAAITTFFGSQLQARVQNHILREYLGEQPVGSAFVIETGHNYHPW
                     LVHAPTMRVPLTIDGTDAVYNATWAALLAIFQHNKNATTDRKIKTVVFPAMGAGCGQV
                     PFESVARQMKQAWDNFNKKTESINWEYAQSRQSAVFGTYAYCPGNSVCRYADTKYIGC
                     GDYRTYCSRSGQVCINPEHQADDVLIQHQANNRFRPGSHIHRINPENPVGNVISGAHS
                     HGSSIVIGAHTHTLNKQYSVSDIK"
     gene            25300..25875
                     /locus_tag="ACHK3A_002726"
     CDS             25300..25875
                     /locus_tag="ACHK3A_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO8740783.1"
                     /translation="MDFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
     gene            complement(25973..26563)
                     /locus_tag="ACHK3A_002727"
     CDS             complement(25973..26563)
                     /locus_tag="ACHK3A_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8740784.1"
                     /translation="MSRIYAYCRVSTLEKTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT         6324 a         6649 c         7992 g         5614 t
ORIGIN      
        1 ctggcggtaa gcaatggaaa ggtctcatga ctcgtcgtga gattgagcgt gaaatctgtt
       61 tgtggggtca gcaatgaaca gagtaaccgc gattatctcc gctctggtta tctgcatcat
      121 cgtctgcctg tcatgggctg ttaatcatta ccgtgataac gccattacct acaaagccca
      181 gcgcgacaaa aatgccagag aactgaagct ggcgaacgcg gcaattactg acatgcagat
      241 gcgtcagcgt gatgttgctg cgctcgatgc aaaatacacg aaggagttag ctgatgcgaa
      301 agctgaaaat gatgctctgc gtgatgatgt tgccgctggt cgtcgtcggt tgcacatcaa
      361 agcagtctgt cagtcagtgc gtgaagccac caccgcctcc ggcgtggata atgcagcctc
      421 cccccgactg gcagacaccg ctgaacggga ttatttcacc ctcagagaga ggctgatcac
      481 tatgcaaaaa caactggaag gaacccagaa gtatattaat gagcagtgca gatagagctg
      541 cccatatcga tgggcaactc atgcaattat tgtgagcaat acacacgcgc ttccagcgga
      601 gtataaatgc ctaaagtaat aaaaccgaga aatccattta cgaatgtttg ctgggtttct
      661 gttttaacaa cattttctgc gccgccacaa attttggctg catcaacagt tttctcctgt
      721 ccaattcccg aaacgaagaa atgatgggtg atggtttcct ttggtgttac tgctgtcggt
      781 ttgtttccaa cagtaaacgt ctgttgagca catcctgtaa taagcattgc cagagcggca
      841 gaaaacaaca tttttttcat cttattatcc tgcattgtta aaaacggcag aatcctatgt
      901 gacaacaatt aaacgatagt taaatggatt gatgaaaatt aaaactatat aggtggatgc
      961 tcagcctatt ggaggagggg gggcactcag aatcctgtgg aatgaaataa accgctctat
     1021 ctgtccatta cccttttagc tgcgctgtat cgtcgccgta ttcccgcatt aaccatgacc
     1081 gtagcccgac ggggaattcc ttctgcgtga gtgtgcggga ataatcaaaa acgatgcaca
     1141 ccgggtttta ctgtgctgac agacgcaggg ttaccctcat agtcgctttt ccggtgcgat
     1201 ggtggaagaa accgggatgt tcatccatca tcactttgga ttgatgtata tgctctcttt
     1261 tctgacgtta gtctccgacg gcaggcttca atgacccagg ctgagaaatt cccagaccct
     1321 ttttgctcaa gagcgatgtt aatttgttca atcatttggt taggaaagcg gatgttgcgg
     1381 gttgttgttc tgcgggttct gttcttcgtt gacatgaggt tgccccgtat tcagtgtcgc
     1441 tgatttgtat tgtctgaagt tgtttttacg ttaagttgat gcagatcaat taatacgata
     1501 cctgcgtcat aattgattat ttgacgtggt ttgatggcgt agatgcacgt tgtgacatgt
     1561 agatgataat tattatcatt tttgcgggtc ctttccggcg atccgacagg ttacggggcg
     1621 gcgacctcgc gggttttcgc tatttatgaa aattttccgg tttaaggcgt ttccgttctt
     1681 cttcgtcata acttaatgtt tttatttaaa ataccctctg aaaagaaagg aaacgacagg
     1741 tgctgaaagc gagctttttg gcctctgtcg tttcctttct ctgtttttgt ccgtggaatg
     1801 aacaatggaa gtcaacaaaa agcagctggc tgacattttc ggtgcgagta tccgtaccat
     1861 tcagaactgg caggaacagg gaatgcccgt tctgcgaggc ggtggcaagg gtaatgaggt
     1921 gctttatgac tctgccgccg tcataaaatg gtatgccgaa agggatgctg aaattgagaa
     1981 cgaaaagctg cgccgggaag ttgaagaact gcggcaggcc agcgagacag atctccagcc
     2041 agggactatt gagtacgaac gccatcgact tacgcgtgcg caggccgacg cacaggagct
     2101 gaaaaatgcc agagactccg ctgaagtggt ggaaaccgca ttctgtactt tcgtgctgtc
     2161 gcggatcgca ggtgaaattg ccagtattct cgacgggatc cccctgtcgg tgcagcggcg
     2221 ttttccggaa ctggaaaacc gacatgttga tttcctgaaa cgggatatca tcaaagccat
     2281 gaacaaagca gccgcgctgg atgaactgat accggggttg ctgagtgaat atatcgaaca
     2341 gtcaggttaa caggctgcgg cattttgtcc gcgccgggct tcgctcactg ttcaggccgg
     2401 agccacagac cgccgttgaa tgggcggatg ccaattacta tctcccaaaa gaatccgcat
     2461 accaggaagg gcgctgggaa acactgccct ttcagcgggc catcatgaat gcgatgggca
     2521 gcgactacat ccgtgaggtg aatgtggtga agtctgcccg tgtcggttat tccaaaatgc
     2581 tgctgggtgt ttatgcctac tttatagagc ataagcagcg caacaccctt atctggttgc
     2641 cgacggatgg tgatgccgag aactttatga aaacccacgt tgagccgacc atccgcgata
     2701 ttccgtcgct gctggcgctg gctccgtggt atggcaaaaa gcaccgggat aacacgctca
     2761 ctatgaagcg tttttccaat ggtcgtggct tctggtgcct gggcggtaaa gcggcaaaaa
     2821 actaccgtga aaagtcggtg gatgtggcgg gttatgatga acttgctgcc tttgatgagg
     2881 atattgaaca ggaaggctct ccgacgttcc ttggcgacaa acgtattgaa ggctcggtct
     2941 ggccaaagtc catccgtggc tccacgccca aagtgagagg cacctgccag attgagcgtg
     3001 cagccagtga atccccgcat tttatgcgtt ttcatgttgc ctgcccgcac tgcggggagg
     3061 agcagtatct taaatttggc gacaaagaga cgccgtttgg cctcaaatgg acgccggatg
     3121 acccctccag cgtgttttat ctctgcgagc ataatgcctg cgtcatccgc cagcaggagc
     3181 tggactttac tgatgcccgt tatatctgcg aaaagaccgg gatctggacc cgtgatggca
     3241 ttctctggtt ttcgtcatcc ggtgaagaga ttgagccgcc ggacagtgtg acctttcaca
     3301 tctggacggc gtacagcccg ttcaccacct gggtgcagat tgtcaaagac tggatgaaga
     3361 cgaaagggga tacgggaaaa cgtaaaacct tcgtgaacac cacgctcggt gagacatggg
     3421 aagcgaaaat tggcgaacgt ccggatgctg aggtgatggc ggagcggaaa gagcattatt
     3481 cagcgcccgt tcctgaccgt gtggcttacc tgaccgccgg tatcgactcc cagctggacc
     3541 gctacgaaat gcgcgtatgg ggatgggggc cgggtgagga aagctggctg attgaccggc
     3601 agattattat gggccgccac gacgatgaac agacgctgct gcgtgtggat gaggccatca
     3661 ataaaaccta tacccgccgg aatggtgcag aaatgtcggt atcccgtatc tgctgggata
     3721 ctggcgggat tgacccgacc attgtgtatg aacgctcgaa aaagcatggg ctgttccggg
     3781 tgatccccat taaaggggca tccgtctacg gaaagccagt ggccagcatg ccacgtaagc
     3841 gaaacaaaaa cggggtttac cttaccgaaa tcggtacgga taccgcgaaa gagcagattt
     3901 ataaccgctt cacactgacg ccggaagggg atgaaccgct tcccggtgcc gttcacttcc
     3961 cgaataaccc ggatattttt gatctgaccg aagcgcagca gctgactgct gaagagcagg
     4021 tcgaaaaatg ggtggatggc aggaaaaaaa tactgtggga cagcaaaaag cgacgcaatg
     4081 aggcgctcga ctgcttcgtt tatgcgctgg cggcgctgcg catcagtatt tcccgctggc
     4141 agctggatct cagtgcactg ctggcgagcc tgcaggaaga ggatggtgca gcaaccaaca
     4201 agaaaacact ggcagattac gcccgtgcct tatccggaga ggatgaatga cgcgacagga
     4261 agaacttgcc gctgcccgtg cggcactgca tgacctgatg acaggaaaac gggtggcaac
     4321 ggtacagaaa gacggacggc gagtggagtt tacggccact tccgtgtctg acctgaaaaa
     4381 atacattgcg gagctggaag tgcagaccgg catgacacag cgacgcaggg gacctgcagg
     4441 attttatgta tgaaaacgtc ctccattccc acccttctgg ggccggacgg catgacatcg
     4501 ctgcgtgaat atgccggtta tcacggcggt ggcagcggat ttggtgggca gttgcgggcg
     4561 tggaacccac cgagtgaaag tgtggatgca gccctgttgc ccaactttac ccgtggcaat
     4621 gcccgcgcgg acgatctggt acgcaataac ggctatgccg ccaacgccat ccagctgcat
     4681 caggatcata tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg gcgctatctg
     4741 ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag
     4801 tttgccgagg atgactgctg ctgcattgac gttgagcgaa aacgcacgtt taccatgatg
     4861 attcgggaag gtgtggccat gcacgccttt aacggtgaac tgttcgttca ggccacctgg
     4921 gataccagtt cgtcgcggct tttccggaca cagttccgga tggtcagccc gaagcgcatc
     4981 agcaacccga acaataccgg cgacagccgg aactgccgtg ccggtgtgca gattaatgac
     5041 agcggtgcgg cgctgggata ttacgtcagc gaggacgggt atcctggctg gatgccgcag
     5101 aaatggacat ggataccccg tgagttaccc ggcgggcgtg cctcgttcat tcacgttttt
     5161 gaacccgtgg aggacgggca gacccgcggt gcaaatgtgt tttacagcgt gatggagcag
     5221 atgaagatgc tcgacacgct gcagaacacg cagctgcaga gcgccattgt gaaggcgatg
     5281 tatgccgcca ccattgagag tgagctggat acgcagtcag cgatggattt tattctgggc
     5341 gcgaacagtc aggagcagcg ggaaaggctg accggctgga ttggtgaaat tgccgcgtat
     5401 tacgccgcag caccggtccg tctgggaggc gcaaaagtgc cgcacctgat gccgggggac
     5461 tcactgaacc tgcagacggc tcaggacacg gataacggct actccgtgtt tgaacagtca
     5521 ctgttgcggt atatcgctgc cgggctgggt gtctcgtatg agcagctttc ccggaattac
     5581 gcccagatga gctactccac ggcacgggcc agtgcgaacg agtcgtgggc gtactttatg
     5641 gggcggcgaa aattcgtcgc atcccgtcag gcgagccaga tgtttctgtg ctggctggaa
     5701 gaggccatcg ttcgccgcgt ggtgacgtta ccttcaaaag cgcgtttcag ctttcaggaa
     5761 gcccgcagcg cctgggggaa ctgtgactgg ataggctccg gtcgtatggc catcgatggt
     5821 ctgaaagaag tacaggaagc ggtgatgctg atagaagccg gactgagtac ctacgagaaa
     5881 gagtgcgcaa aacgcggtga cgactatcag gaaatttttg cccagcaggt ccgtgaaacg
     5941 atggagcgcc gtgcagccgg tcttaaaccg cccgcctggg cggctgcggc atttgaatcc
     6001 ggactgcgac aatcaacaga ggaggagaag agtgacagca gagctgcgta atctcccgca
     6061 tattgccagc atggctttta atgagccgct gatgcttgaa cccgcctatg cgcgggtttt
     6121 cttttgtgcg cttgcaggcc agcttgggat cagtcgcctg acggatgcag tatccggcga
     6181 cagcctgact gccggagagg cacccgcggc gctggcgtta tccggtgatg atgacggacc
     6241 acgacaggcc cgcagttatc aggtcatgaa cggcatcgcc gtgctgccgg tgtccggtac
     6301 gctggtcagc cggacgcggg cgctgcagcc gtattcggga atgaccggtt acaacggcat
     6361 tatcgcccgt ctgcaacagg ctgccagcga tccgatggtg gacggcattc tgctcgatat
     6421 ggacacaccg ggcgggatgg tggcgggagc atttgactgt gctgacatca tcgcccgtgt
     6481 gcgtgacata aagccggtat gggcgctggc caacgacatg aactgcagtg caggtcagct
     6541 gcttgccagc gccgcctccc ggcgtctggt cacgcagacc gcccggacag gctccatcgg
     6601 cgtcatgatg gctcacagta attacggcgc tgcgctggag aaacagggcg tggaaatcac
     6661 gctgatttac agcggcagcc ataaggtgga tggcaacccc tacagccatc taccgggtga
     6721 tgtccgggag acactgcagt cccggatgga tgcaacccgc cggatgtttg cgcagaaggt
     6781 gtcggcatat accggcctgt ccgtgcaggc tgtgctggat accgaggctg cagtgtacag
     6841 cggtcaggag gccattgatg ccggactggc tgatgaactt gtcaacagca ccgatgcgat
     6901 caccgttatg cgtgatgcac tggatgcacg taaatcccgt ctctcaggag ggcgaatgac
     6961 caaagagact caatcaacaa ctgtttcagc cactgcttcg caggctgacg ttactgacgt
     7021 ggtgcaagcg acggagggcg aaaacgccag cgcggcgcag ccggacgtga acgcgcagat
     7081 caccgctgcg gttgcggcag aaaacagccg cattatgggg atcctcaact gtgaggaagc
     7141 tcacggacgc gaagaacagg cacgtgtgct ggccgaaacc cccggtatga ccgtggaaac
     7201 ggcccgccgc attctggcag ctgcaccaca gagtgcacag gcgcgcagtg acactgcgct
     7261 ggatcgtctg atgcaggggg caccggcacc actggctgca ggtaacccgg catctgatgc
     7321 cgttaacgat ttgctgaaca caccagtgta agggatgttt atgacgagca aagaaacctt
     7381 tacccattac cagccgctgg gcaacagtga cccggcacat acggcaaccg cgcccggcgg
     7441 attgagtgcg aaagcgcctg caatgacccc gctgatgctg gacacctcca cccgtaagct
     7501 ggttgcgtgg gatggcacca ccgacggtac tgccgttggc attctggcgg ttgatgctga
     7561 ccagaccagc accacgctga cgttctacaa gtccggcacg ttccgttatg aggatgtgct
     7621 ctggccggag gctgccagcg acgagacgaa aaaacggacc gcgtttgccg gaacggcaat
     7681 cagcatcgtt taaccttacc cttcatcact aaaggccgcc tgtgcggctt tttttacggg
     7741 atttttttat gtcgatgtac acaaccgccc agctgctggc ggcaaatgag cagaaattta
     7801 agtttgatcc gctgtttctg cgtctctttt tccgtgagag ctatcccttc accacggaga
     7861 aagtctatct ctcacaaatt ccgggactgg taaacatggc gctgtacgtt tcgccgattg
     7921 tttccggtga ggttatccgt tcccgtggcg gctccacctc tgaatttacg ccgggatatg
     7981 tcaaacccaa gcatgaggtg aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc
     8041 cgcagaatct ggcggacccg gcttaccgcc gccgtcgcat catcatgcag aacatgcgag
     8101 acgaagagct ggccattgct caggtcgaag agatgcaggc agtttctgct gtgcttaagg
     8161 gcaaatacac catgaccggt gaagccttcg atccggttga ggtggatatg ggccgcagtg
     8221 cggcgaacaa catcacacag tccggtggta cggagtggag caagcgtgac aagtccacgt
     8281 atgacccgac cgacgatatc gaagcctatg cgctgaacgc cagcggcgtg gtgaatatca
     8341 tcgtgtttga tccgaaaggc tgggcgctgt tccgttcctt caaagccgtc aaggagaagc
     8401 tggatacccg tcgcggctct cattccgagc tggagacagc ggtaaaagac ctgggcgaag
     8461 cggtgtccta taaggggatg tatggcgata cggcgatcgt cgtgtattcc ggacagtacg
     8521 tggaaaacga cgtcaaaaag aacttcctgc cggacaacac gatggtgctg gggaacactc
     8581 aggcacgcgg tctgcgcacc tatggctgca ttcaggatgc ggacgcacag cgcgaaggta
     8641 ttaacgcctc tgcccgctac ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt
     8701 tcaccatgat tcagtcagca ccgctgatgc tgctggctga tcctgatgcg ttcgtgtccg
     8761 tacaactggc gtaatcatgg cccttcgggg ccattttctc tctgtggagg agtccatgac
     8821 gaaagatgaa ctgattgccc gtcttcaggt gctgggtgag caactgaacc gtgatgtcag
     8881 cctgacgggg acgaaagaag aactggtgct ccgtgtggca gagctggaag aggagcttga
     8941 tgacacggat gacgctgccg gtcaggacac atctgtcagc ccggaaaatg cgctgaccgg
     9001 acatgaaaat gaggtggtat cagcgcagcc ggataccgtg attgatacgg ctgctctggt
     9061 cacggtcgtg gcactggtga cgctgcatac tgatgcactt cacgccacgc gggatgagcc
     9121 tgtggcattt gtgctgccgg gaacggcgtt tcgtgtctct gccggtgtgg cagccgaaat
     9181 gacagaacat ggcctggcca gaatgcaata acgggaggcg ctgtggctga ttccgataac
     9241 ctgttcgatg ctgccattgc ccgcgccgat gaaacgatac gcgggtacat gggaacgtca
     9301 gccaccatga catccggtga gctgtccggt gctgtgatac gtggtgtttt tgatgaccct
     9361 gaaaatatca gctatgccgg acagggggtg cgcgttgaag gctccagccc gtccctgttt
     9421 gtccggactg atgatgtgcg gcagctgcgg cgtggagaca cactgaccat cggcgaggaa
     9481 aacttctggg tggaccggat ttcgccggat gatggcggaa gctgtcatct ctggcttggg
     9541 cgtggcgtgc cgcctgccgt taaccgtcgc cgctgaaagg gggatgtatg gccataaaag
     9601 gtcttgagca ggccgttgaa aacctcagcc gtatcagcaa aacggcggtg cctggtgccg
     9661 ccgcaatggc cattaaccgc gttgcgtcat ccgcgatatc gcagtctgcg tcacaggttg
     9721 cccgtgagac aaaggtacgc cggaaactgg taaaggaaag ggccaggctg aaaagggcca
     9781 cggtcaaaaa tccgcaggcc agaatcaggg ttaaccgggg ggatttgccc gtaatcaagc
     9841 tgggtaacgc gcgggttgtc ctttcccgcc gcagacgtcg taaaaagggg cagcgttcat
     9901 ccctgaaagg tggcggcagc gtgcttgtgg tgggaaaccg tcgtatttcc ggcgcgttta
     9961 ttcagcaact gaaaaatggc cggtggcatg tcatgcagcg tgtggccggg aaaaaccgtt
    10021 accccattga tgtggtgaaa atcccgatgg cggtgccgct gaccacggcg tttaaacaga
    10081 atattgaacg gatacggcgt gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc
    10141 aactgagaat ggtaataaag cgatgaaaca tactgaactc cgtgcagccg tactggatgc
    10201 actggagaag catgacaccg gggcgacgtt ttttgatggt cgccccgctg tttttgatga
    10261 ggcggatttt ccggcagttg ccgtttatct caccggcgct gaatacacgg gcgaagagct
    10321 ggacagcgat acatggcagg cggagctgca tattgaagtt ttcctgcctg ctcaggtgcc
    10381 ggattcagag ctggatgcgt ggatggagtc ccggatttat ccggtgatga gtgatatccc
    10441 ggcactgtca gatttgatca ccagtatggt ggccagcggc tatgactacc ggcgcgacga
    10501 tgatgcgggc ctgtggagtt cagccgatct gacttatgtc attacctatg aaatgtgagg
    10561 acgctatgcc tgtaccaaat cctgtaatgc cggtgaaagg tgccgggacc accctgtggg
    10621 tttataaggg aagcggtgac ccttatgcga atccgctttc agacgttgac tggtcgcgtc
    10681 tggcaaaagt taaagacctg acgcccggcg aactgaccgc tgagtcctat gacgacagct
    10741 atctcgatga tgaagatgca gactggactg cgaccgggca ggggcagaaa tctgccggag
    10801 ataccagctt cacgctggcg tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt
    10861 ggtttaatga aggggatacc cgagcctata aaatccgctt cccgaacggc acggtcgatg
    10921 tgttccgcgg ctgggtcagc agtatcggta aggcggtgac ggcgaaggaa gtgatcaccc
    10981 gcacggtgaa agtcaccaac gtgggccgtc cgtcgatggc agaagatcgc agcacggtga
    11041 cggcgacaac cggcatgact gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca
    11101 ccacgctgac cgtggcattc cagccggaag gcgcaaccga caagagcttc cgtgcggtgt
    11161 ctgcggataa aacaaaagcc accgtgtcgg tcagtggtat gaccatcacc gtgaaaggtg
    11221 ttgctgcagg caaggtcaac attccggtcg tatccggtaa tggtgaactt gctgcggttg
    11281 cagaaatcac cgtcaccgcc agttaatccg gggagtcagc gatgttcctg aaaaccgaat
    11341 catttgaaca taacggtgtg accgtcacgc tttctgaact gtcagccctg cagcgaattg
    11401 agcatctcgc cctgatgaaa cggcaggcag aacaggcgga gtcagacagc aaccggaagt
    11461 ttactgtgga agacgccatc agaaccggcg cgtttctggt ggcgatgtcc ctgtggcata
    11521 accatccgca gaagacgcag atgccgtcca tgaatgaagc cgttaaacag attgagcagg
    11581 aagtgcttac cacctggccc acggaggcaa tttctcatgc tgaaaacgtg gtgtaccggc
    11641 tgtctggtat gtatgagttt gttgtgaata atgcccctga acagacagag gacgccgggc
    11701 ccgcagagcc tgtttctgcg ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact
    11761 ggcgcgagag atggggcgac ccgactggcg tgccatgctt gccgggatgt catccacgga
    11821 gtatgccgac tggcaccgct tttacagtac ccattatttt catgatgttc tgctggatat
    11881 gcacttttcc gggctgacgt acaccgtgct cagcctgttt ttcagcgatc cggatatgca
    11941 tccgctggat ttcagtctgc tgaaccggcg tgaggctgac gaagagcctg aagatgatgt
    12001 gctgatgcag aaagcggcag ggcttgccgg aggcgtccgc tttggcccgg acgggaatga
    12061 agttatcccc gcttccccgg atgtggcgga catgacggag gatgacgtaa tgctgatgac
    12121 agtatcagaa gggatcgcag gaggagtccg gtatggctga accggtaggc gatctggtcg
    12181 ttgatttaag tctggatgcg gccagatttg acgagcagat ggccagagtc aggcgtcatt
    12241 tttccggtac ggaaactgat gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc
    12301 gacaggcgct ggctgcacag aaagcgggga tttccgtcgg gcagtataaa gccgccatgc
    12361 gtatgctgcc tgcacagttc accgacgtgg ccacgcagct tgcaggcggg caaagcccgt
    12421 ggctgatcct gctgcaacag gggggtcagg ttaaggactc cttcggcggg atgatcccca
    12481 tgttcagggg gcttgccggt gcgatcaccc tgccgatggt gggggccacc tcgctggcgg
    12541 tggcgaccgg tgcgctggcg tatgcctggt atcagggcaa ctcaaccctg tccgatttca
    12601 acaaaacgct ggtcctttcc ggcaatcagg cgggactgac ggcagatcgt atgctggtcc
    12661 tgtccagagc cgggcaggcg gcagggctga cgtttaacca gaccagcgag tcactgacgg
    12721 cgctggtgaa tgccggtgtg cgtggtggtg agcagtttga ggcgatcagc cagagtgtgg
    12781 cgcgtttctc ctctgcatcc ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc
    12841 tgaccacaga cccgacgtcg ggactgacag cgatggcacg tcagttccat aacgtgacgg
    12901 cggagcagat tgcgtatgtt gctcagttgc agcgttccgg agatgaagcc ggggcattgc
    12961 aggcggcgaa cgaggccgca acgaaagggt ttgatgacca gacccgccgc ctgaaagaga
    13021 acatgggcac gctggagacc tgggcagaca ggacagcacg ggcattcaaa tccatgtggg
    13081 attcggtgct ggatattggt cgcccggaca ctgcccaggg aatgctggag aaagcagaaa
    13141 aggcttttga tgaggcggac aaaaaatggc agtggtatca gagccggagc caccggcgcg
    13201 gtaaaacctc agcatttctt gccaatctcc ggggagcatg ggaggacaga gcgaatgcgc
    13261 aacttgggct ttcagccgcc acgttgcagg ccgatcttga aaaggccaga gagatggcag
    13321 caaaggactg ggccgagtct gaggcatcac ggctgaaata taccgaagag gcgcagaagg
    13381 cttacgaacg gctgcagacg ccgctggaga aatataccgc ccgtcaggaa gaactgaaca
    13441 aggcactgaa agacgggaaa atcctgcagg cggattacaa cacgctgatg gcggcggcga
    13501 aaaaggatta tgaagcgacg ctgaaaaagc cgaaacagtc cggcgtgaag gtgtctgcgg
    13561 gcgatcgtca ggaagacagt gctcatgctg ccctgctgac gcttcaggct gaactccgga
    13621 cgctggagaa gcatgccgga gcaaatgaga aaatcagcca gcagcgccgg gatttgtgga
    13681 aggcggagag ccagttcgcg gtactggagg aggcggcgca acgtcgccag ctgtctgcac
    13741 aggagaaatc cctgctggcg cataaagatg agacgctgga gtacaaacgc cagctggctg
    13801 cacttggcga caaggttacg tatcaggagc gcctgaacgc gctggcgcag caggcggata
    13861 aattcgcaca gcagcaacgg gcaaaacggg ccgccattga tgcgaaaagc cgggggctga
    13921 ctgaccggca ggcagaacgg gaagccacgg aacagcgcct gaaggaacag tatggcgata
    13981 atccgctggc gctgaataac gtcatgtcag agcagaaaaa gacctgggcg gctgaagacc
    14041 agcttcgcgg gagctggatg gcaggcctga agtccggctg gagtgagtgg gaagagagcg
    14101 ccacggacag tatgtcgcag gtaaaaagtg cagccacgca gacctttgat ggtattgcac
    14161 agaatatggc ggcgatgctg accggcagtg agcagaactg gcgcagcttc acccgttccg
    14221 tgctgtccat gatgacagaa attctgctta agcaggcaat ggtggggatt gtcgggagta
    14281 tcggcagcgc cattggcggg gctgttggtg gcggcgcatc cgcgtcaggc ggtacagcca
    14341 ttcaggccgc tgcggcgaaa ttccattttg caaccggagg atttacggga accggcggca
    14401 aatatgagcc agcggggatt gttcaccgtg gtgaatttgt cttcacgaag gaggcaacca
    14461 gccggattgg cgtggggaat ctttaccggc tgatgcgcgg ctatgccacc ggcggttatg
    14521 tcggtacacc gggcagcatg gcagacagcc ggtcgcaggc gtccgggacg tttgagcaga
    14581 ataaccatgt ggtgattaac aacgacggca cgaacggtca gataggtccg gctgctctga
    14641 aggcggtgta tgacatggcc cgcaagggtg cccgtgatga aattcagaca cagatgcgtg
    14701 atggtggcct gttctccgga ggtggacgat gaagaccttc cgctggaaag tgaaacccgg
    14761 tatggatgtg gcttcggtcc cttctgtaag aaaggtgcgc tttggtgatg gctattctca
    14821 gcgagcgcct gccgggctga atgccaacct gaaaacgtac agcgtgacgc tttctgtccc
    14881 ccgtgaggag gccacggtac tggagtcgtt tctggaagag cacgggggct ggaaatcctt
    14941 tctgtggacg ccgccttatg agtggcggca gataaaggtg acctgcgcaa aatggtcgtc
    15001 gcgggtcagt atgttgcgtg ttgagttcag cgcagagttt gaacaggtgg tgaactgatg
    15061 caggatatcc ggcaggaaac actgaatgaa tgcacccgtg cggagcagtc ggccagcgtg
    15121 gtgctctggg aaatcgacct gacagaggtc ggtggagaac gttatttttt ctgtaatgag
    15181 cagaacgaaa aaggtgagcc ggtcacctgg caggggcgac agtatcagcc gtatcccatt
    15241 caggggagcg gttttgaact gaatggcaaa ggcaccagta cgcgccccac gctgacggtt
    15301 tctaacctgt acggtatggt caccgggatg gcggaagata tgcagagtct ggtcggcgga
    15361 acggtggtcc ggcgtaaggt ttacgcccgt tttctggatg cggtgaactt cgtcaacgga
    15421 aacagtgacg ccgatccgga gcaggaggtg atcagccgct ggcgcatcga gcagtgcagc
    15481 gaactgagcg cggtcagtgc ctcttttgta ctgtccacgc cgacggaaac ggacggtgcc
    15541 gtttttccgg gacgtatcat gctggccaac acctgcacct ggacctatcg cggtgatgag
    15601 tgcggttatc acggtccggc ggtcgcggat gaatatgatc agccgacgtc cgatatcacg
    15661 aaggataaat gcagcaaatg cctgagcggc tgtaagtttc gcaataacgt cggcaacttt
    15721 ggcggcttcc tttccattaa caaactttcg cagtgaatcc catgacagag acagaatcag
    15781 cgattctggc gcacgcccgg cgatgtgcgc cagcggagtc gtgcggcttc gtggtgagaa
    15841 caccggaggg ggaaagatat tttccctgcg tgaatatctc cggtgagccg gaggcgtatt
    15901 tccggatgtc gccggaggac tggctgcggg cagagatgca gggtgagatt gtggcgctgg
    15961 tccacagcca ccccggtggt ctgccctggc tgagtgagac cgaccggcgg ctgcaggtgc
    16021 agagtgattt gccgtggtgg ctggtttgcc ggggggcgat tcacaagttc cgctgtgtgc
    16081 cacatctttc cgggcggcgc tttgagcacg gggtgacgga ctgttacacg ctgttccggg
    16141 atgcttacca tctggcgggg attgagatgc cggattttca tcgcgaggat gactggtggc
    16201 gtcacggtca gaatctctat ctggataatc tggaggccac agggctgtat caggtgccgt
    16261 tgtcatcagc acaaccgggc gatgtgctgc tgtgctgttt tggttcatcg gtgccgaatc
    16321 atgccgccat ttactgtggt gacagcgagc tgctgcacca tattcctgaa caactgagca
    16381 aacgagagag gtacaccgac aaatggcagc gacgcacaca ctccctctgg cgtcaccggg
    16441 aatggcacgc atctgccttt acggggattt gcaacgattt ggccgccgca tcgaccttcg
    16501 tgtgaaaacg ggggctgaag ccatccgcgc actggccaca cagctcccgg cgtttcgtca
    16561 gaaactgagc gacggctggt atcaggtacg gattgccggg cgggacgtca gcacgtccgg
    16621 gttaacggcg cagttacatg agactctgcc tgacggcgct gtgattcata ttgttcccag
    16681 agtcgccggg gccaagtcag ggggcgtatt ccagattgtt ctgggggctg ccgccattgc
    16741 cggatcattc tttactgccg gagccaccct tgcagcatgg ggggcagcca ttggggccgg
    16801 tggtatgacc ggcatcctgt tttctctcgg tgccagtatg gtgctcggtg gtgtggcgca
    16861 gatgctggca ccgaaagcca gaactccccg tacacagaca acggataacg gtaagcagaa
    16921 cacctatttc tcctcactgg ataacatggt tgcccagggc aatgttctgc ctgttctgta
    16981 cggggaaatg cgcgtggggt ctcgcgtggt ttctcaggag atcagcacgg cagacgaagg
    17041 ggatggtggt caggttgtgg tgattggtcg ctgatgaaaa acgtttatgt gaaaccgcct
    17101 ccgggcggtt ttgtcgttta tggagcgtga ggaatgggta aaggcagcag taaggggcat
    17161 actccgcgcg aagcgaagga caacctgaag tccacgcagt tgctgagtgt gatcgatgcc
    17221 atcagcgaag ggccgattga aggtccggtg gatggattaa aaagcgtgct gctgaacagt
    17281 acgccagtgc tggacagtga ggggaatact aacatctccg gcgtcacggt ggtgttccgg
    17341 gccggtgagc aggagcagac accgccggag ggatttgaat cctccggctc cgagacggtg
    17401 ctgggtacgg aagtgaaata cgacacgccg atcacctgga ccatcacgtc ggcaaacatt
    17461 gaccgactgc gtgttacctt cggcgtgcag gcactggtgg aaaccacctc aaagggggac
    17521 aggaatccgt cggaagtccg cctgctggtt cagatacagc gtaatggtgg ctgggtgacg
    17581 gaaaaagaca tcaccattaa gggcaaaacc acctcgcagt atctggcctc ggtggtggtg
    17641 ggtaacctgc cgccgcgccc gttcagtata cggatgcgca ggatgacgcc ggacagcacc
    17701 acagaccagc tgcagaacaa aacgctctgg tcgtcataca ccgaaatcat cgatgtgaaa
    17761 cagtgctacc cgaacacggc actggtcggt gtgcaggtgg actcggagca gttcggcagc
    17821 cagcaggtga gccgtaatta tcatctgcgc gggcgtattc tgcaggtgcc gtcgaactat
    17881 aacccgcaga cgcggcaata cagcggtatc tgggacggaa cgtttaaacc ggcatacagc
    17941 aacaacatgg cctggtgtct gtgggatatg ctgacccatc cgcgctacgg catggggaaa
    18001 cgtcttggtg cggcggatgt ggataaatgg gcgctgtatg tcatcggcca gaattgcgac
    18061 cagtcggtgc cggacggctt tggcggcacg gagccgcgca tcacctgtaa tgcctggctg
    18121 accacacagc gcaaggcgtg ggatgtgctc agtgatttct gctcggcgat gcgctgtatg
    18181 ccgatatgga acgggcagac gctgacgttc gtgcaggacc gaccatcaga taaggtgtgg
    18241 acctataacc gcagtaatgt ggtgatgccg gatgatggcg cgccgttccg ctacagcttc
    18301 agcgccctga aggaccgcca taatgccgtt gaggtgaact ggattgaccc ggataacggc
    18361 tgggagacgg cgacagagct tgtggaggac acgcaggcca ttgcccgtta cggtcgtaac
    18421 gtcacgaaga tggatgcctt tggctgtacc agtcgggggc aggcgcaccg cgccgggctg
    18481 tggctgatta aaacggaact gctggaaacg cagaccgtgg acttcagcgt gggtgcggaa
    18541 gggcttcgcc atgtaccggg ggatgtcatt gaaatctgcg atgatgacta tgcgggtatc
    18601 agcaccggcg ggcgcgtgct ggcggtgaac agccagaccc ggacgctgac gctcgaccgt
    18661 gaaatcacgc tgccatcctc cggtaccacg ctgataagcc tggttgacgg aagtggcaat
    18721 ccggtcagcg tggaggtcca gtccgtcacc gacggcgtga aggtaaaagt gagccgtgtt
    18781 cctgacggcg ttgccggata cagcgtgtgg gggctgaagc tgccgacgct gcgccagcga
    18841 ctgttccgct gcgtgagtat ccgtgagaac gacgacggca cgtatgccat caccgccgtg
    18901 cagcatgtgc cggaaaaaga ggccatcgtg gataacgggg cgcactttga cggcgaccag
    18961 agcggcacgg tgaatggtgt cacgccgcca gcggtgcagc acctgactgc cgaagtcacc
    19021 gcagacagcg gggaatatca ggtgctggcg cgctgggaca caccgaaggt ggtgaagggc
    19081 gtgagcttcc tgctccgtct gaccgtaaca gcggacgacg gcagtgagcg gctggtcagc
    19141 acggcccgga cgacggaaac cacataccgc ttcacacaac tggcgttggg gaactacagg
    19201 ctgacagtcc gggcggtaaa tgcgtgggga cagcagggcg atccggcatc ggtatcgttc
    19261 cggattgccg caccggcagc gccgtctcgg attgagctga caccgggcta ttttcagata
    19321 accgccacgc cgcatcttgc ggtttatgac ccgacggtac agtttgagtt ctggttctcg
    19381 gaaaaacgga ttgcggatat caggcaggtt gaaaccagcg cgcgttatct tggtacggca
    19441 ctgtactgga tagccgccag tatcaatatc aaaccgggcc atgattatta tttttacgtt
    19501 cgcagtgtga acaccgttgg caaatcggca ttcgtggagg ctgtcggtca gccgagtgat
    19561 gatgcatcag gctatctgga ttttttcaaa ggcgagatag ggaaaaccca tctggctcag
    19621 gagctgtgga cgcagattga taacggtcag cttgcgcctg acctgactga aatcaggacg
    19681 tccataacgg atgtcagcaa tgaaataaca cagaccgtca ataagaaact ggaagaccag
    19741 agtgcagcga tccagcagat acagaaggtt caggttgata caaataataa cctgaacagc
    19801 atgtgggcag tgaagctgca gcagatgcag gacggacgcc tttatattgc gggtatcggt
    19861 gccggtattg agaacacccc tgacggcatg cagagtcagg tgctgctggc agcagacagg
    19921 attgcgatga ttaatcctgc gaatggcaac acaaagccga tgtttgttgg tcagggcgat
    19981 cagatattca tgaatgaagt gttcctgaaa cgcctgacgg cccccaccat taccagcggc
    20041 ggtaatcctc cggcattttc cctgacatca gacgggagac tgacggcgaa aaatgcggat
    20101 atcagtggca gtgtgaatgc gaactcagga acgctcaaca atgtcacgat taaccagaac
    20161 tgtacgatta agggcatgct ggaggcgacc caggtcagag gagatttcgt taaagctgta
    20221 tcaaaagcct tcccgaaaaa agtcggtacg tggggtaaca cggaaacacc aaacggtacg
    20281 gttacagtca ccatcagcga tgatcataac tttgaccgcc agattattat tccgcccatt
    20341 atttttaacg gtatagcgta tgacgatccg gggagcggaa ataacccagg aggcacgcga
    20401 tacacgggtt atggttttga agttcgcaaa aacggcgtat taatcgcatc cagagaaact
    20461 aaaggggcca ttcccggtag ttacagtgca gttattgata tgccgagtgg caggggaagc
    20521 gtcactctgg agtttaagat tttccagaaa ggcaatcagg gggcaggcaa tatcaccgac
    20581 tgtacggtga ttgtgaccaa aaaagccgct tccggcatca gtattcgttg aaatatttat
    20641 aaccccaata aagggcgtca ggaatgacgc cttttttatt gcagaaaagc gagaggtaat
    20701 tatgcgtaaa gtttgtgcag caattttgtc cgcagccatt tgtctggccg tatccggtgc
    20761 gcctgcatgg gcgtctgaac atcagtccac gctgagcgcg gggtatcttc atgcctcgac
    20821 gaacgtcccc ggcagcgatg atctgaacgg gattaacgtg aaataccgtt atgagtttac
    20881 ggacacactg gggctggtga cgtcattcag ctatgcagga gacaagaatc gccagctgac
    20941 ccgttacagc gatacccgct ggcatgaaga ttccgttcgt aaccgctggt tcagcgtaat
    21001 ggcggggccg tctgtgcgcg tgaatgaatg gttcagcgcg tatgcgatgg cgggtgtagc
    21061 ttacagccgt gtgtcgactt tctccgggga ttatcttcgc gtaactgaca acaaggggaa
    21121 aaagcacgat gtgctgaccg gaagtgatga cggtcgccac agcaacacgt ctctggcgtg
    21181 gggggctggc gtgcagttta acccgaccga atccgtggcc attgatattg cttatgaagg
    21241 ctccggcagt ggtgactggc gcactgacgg tttcatcgtg ggtgtcggtt ataaattctg
    21301 attagccagg taacacagtg ttatgacagc ccgccggttc aggcgggctt ttttgtgggg
    21361 tgaatatggc agtaaagatt tcaggtgtac tgaaagacgg cacaggaaaa ccggtacaga
    21421 actgcacaat ccagctgaaa gcaaaacgta acagcaccac ggtggtggtg aacacgctgg
    21481 cctcagaaaa tccggatgaa gccgggcgtt acagcatgga cgttgagtac gggcagtaca
    21541 gcgttattct gttggtggag ggattcccgc cgtcacatgc cgggaccatc accgtgtatg
    21601 aagattcccg acccggtacg ctgaatgatt ttctcggtgc catgacggag gatgatgccc
    21661 gtcctgaggc actgcgccgt tttgaactga tggtggaaga ggtggcgcgt aacgcgtccg
    21721 cggtggcaca gaacacggca gccgcgaaga agtcagccag cgatgccagc acatcagccc
    21781 gtgaggcggc aacccatgcg actgatgctg cagattcagc acgtgcagcc agcacgtcag
    21841 ccggacaggc cgcttcgtcg gctcagtcag cgtcttccag cgcaggaacg gcatcaacaa
    21901 aggccactga agcctcaaaa agtgctgccg ctgcagagtc ctcaaaaagc gtggcagcta
    21961 ccagtgccgg tgcggcgaaa acgtcagaaa cgaatgccgc agcgtcagaa caatcagccg
    22021 ccacttctgc atccaccgcg accacgaaag cgtcagaagc tgccacctca gcccgggatg
    22081 cggcggcctc aaaagaggca gcgaaatcat cagaaacgaa cgcatccttg agcgccagta
    22141 gcgcagcttc ctcggcaacg gcggcaggaa attccgcgaa ggcggcaaaa acgtccgaga
    22201 cgaacgccag gtcttctgag acggcagcgg gacagagcgc ctcagctgcg gcaggctcaa
    22261 aaacagcggc tgcgtcgtct gccagtgccg cgtcaacaag tgccgggcag gcctcagcca
    22321 gtgccaccgc cgccggaaaa tcggcagaaa gcgccgcatc atccgcttca acagccacaa
    22381 cgaaggctgg cgaagccact gagcaggcca cggcagcagc gaggtcagct tccgcagcga
    22441 agacatccga aacgaacgcg aaagcgtcgg aaacccgtgc agaatcctca aaaacggcag
    22501 cggcatcgtc cgccagttcg gcggcgtcat cggcatcatc ggcgtctgct tcaaaagatg
    22561 aggcgaccag acaggcgtca gcagcgaagg gcagcgccac gacggcatcc acgaaggcga
    22621 cagaggctgc tggtagtgcg aaggcagcag ctcagagcaa aagtacggcg gaatctgcag
    22681 caacgcgcgc tgagacagcg gcaaaacggg cagaggatat tgcatccgcc gtggcgcttg
    22741 aggatgcgag cacgacgaaa aaggggatag tacagctcag tagtgcgact aacagcactt
    22801 ccgagtcact ggcggcaaca ccaaaagccg ttaaggccgc gtatgacctg gctagcggga
    22861 aatacaccgc acaggatgca acgacagcac agaaagggat aatccagcta agcagcgcga
    22921 ccaacagcac gtctgaaacg ctggcggcaa cgccaaaggc agtaaaagca gccaatgaca
    22981 atgctgagaa acgtctgcag aaagatcaga acggtgcgga tatccctggc aaagacacct
    23041 ttacgaaaaa tattggtgcc tgccgtgcct tcggtgggtc agtaagcaca acaacaggaa
    23101 actggacgac tgcacagttt atcgagtggc tggattctca gggagcattt aaccatccat
    23161 actggatgtg caagggttcc tggtcttatg gcaataataa aatcattact gatactggct
    23221 gcggtaatat tcatctcgcc ggagctgtca ttgaagtaat ggggataaag tcagcgatga
    23281 cgatccgcat taccactccg accacatcca gcggtggcgg aacaaccaac gcccagttta
    23341 cctatattaa tcacggaaca gattattcac ctggctggcg aagggactat aactccagaa
    23401 ataagccaac ggcatcagag atcggggcgc taccgtcagg tggaacagca gtatcatcag
    23461 ttaatctggc ttcaaaaggt cgggtagccg cgctgacaga caatacgcag ggggcagcag
    23521 gtcttgagtt atacgaggtg tataacaacg gatatccaac aacgtatgga aatatcattc
    23581 acctgaaagg gatgacagcc gttggcgaag gtgagttact catcggctgg agtggtacaa
    23641 gcggtgctca tgctccggca tttattcgtt cacgacggga tacgaccgac gcaaactggt
    23701 cgccgtgggc gcagctttac acctcggctc atcctcctgc agagttttat ccagtcggtg
    23761 caccaatccc gtggccatca gataccgttc cgtctggtta tgtcctgatg caggggcaga
    23821 cttttgacaa atctgcatac ccgaaacttg cagccgctta tccgtcaggc gtgatccctg
    23881 atatgcgtgg ctggacgatt aagggcaaac ctgccagtgg tcgggccgta ttgtctcagg
    23941 aacaggacgg cattaaatcg cacacccaca gcgccagcgc atccagtacg gatttgggga
    24001 cgaaaaccac atcgtcgttt gattacggca ctaaatccac gaataacatc ggggcgcata
    24061 cgcacagtgt gagcggtaca gccgcaagtg ccggaaacca tactcatagt gtcacaggcg
    24121 catcagcagt cagccagtgg tcacaaaatg ggtcagtaca taaggtagtg tctgcggcca
    24181 gtgtgaatac aagtgctgca ggagcgcaca ctcatagtgt cagcggcaca gctgcatctg
    24241 caggtgctca cgcacatact gtcggtattg gtgctcatac gcactcggtt gcgattggct
    24301 cacatggaca caccatcacc gttaacgctg ctggtaacgc ggaaaacacc gtcaaaaaca
    24361 tcgcatttaa ctatattgtg aggcttgcat gattacgctc attctttctg caccagtaac
    24421 cgaaatggct gaagcattta agcgggtatt tgcaaatgca gataatgtga atattgtcgg
    24481 aaagccattt gaaacaatca gagaatttga ctgcatggta agtgcggcaa atagtttcgg
    24541 cctgatggat ggcggtgttg atgccgccat taccacattc ttcggttctc agctacaggc
    24601 ccgcgttcag aatcatattc ttcgtgaata tctcggcgag cagcctgtag gttctgcatt
    24661 tgttattgaa acggggcata attatcaccc ctggctggta catgcgccaa caatgcgtgt
    24721 tccgttgaca attgacggaa cagacgctgt atataacgca acttgggcag ctctacttgc
    24781 tatctttcag cacaataaaa acgcaacgac agacaggaaa ataaagacgg tggtattccc
    24841 tgcaatggga gccggatgtg gtcaggtgcc gtttgaaagc gttgcccggc agatgaagca
    24901 ggcatgggat aactttaata aaaaaacaga atcaattaac tgggaatacg cacaatcccg
    24961 ccagtcggca gtatttggca catatgcata ctgtccgggt aattctgttt gccgttatgc
    25021 ggatactaaa tatattggat gcggcgatta tcggacgtat tgctcacgtt ccgggcaggt
    25081 ctgtattaac cctgaacatc aggctgatga tgtactgata caacatcagg ctaataaccg
    25141 gtttcgccct ggttcgcata tacaccggat taatccagaa aatcctgtag gtaatgtcat
    25201 ctctggcgca catagccacg gaagtagcat cgttattggt gctcacaccc atacgctcaa
    25261 taaacaatat tctgtctctg atattaagta gaggtgaaca tggatttcag aatgagtgaa
    25321 caaccacgga ccataacaat ttataatctg ctggccggaa ctaatgaatt tattggtgaa
    25381 ggtgacgcat acattccgcc tcatacaggt ctgccagcaa acagtaccga tattgcaccg
    25441 ccagatattc cggctggttt cgtggctgtt ttcaacagtg atgagtcatc gtggcatctc
    25501 gttgaagatc atcggggtaa aacggtttat gacgtggctt ccggcgacgc gttatttatt
    25561 tctgaactcg gctcattacc ggaaaatgtc acctggttat ccccggaagg ggagtttcag
    25621 aagtggaacg gcacagcctg ggtgaaggat acggaagcag aaaaactgtt ccggatccgg
    25681 gaggcggaag aaacaaaaaa cagcctgatg caggtagcca gtgagcatat agcgccgctt
    25741 caggatgctg tggatctgga gatcgcaacg gagaaagaaa ccttgttgct ggaagcctgg
    25801 aaaaagtatc gggtgttgct gaaccgtgtt gatacatcaa ctgcaccgga tattgaatgg
    25861 ccagccctac cgtagggtaa gatatataaa ttctataatt agaagtatct ttccatttaa
    25921 ggctaggaag gggggcttgg aaaacgtaag gaatctcaca ccgagattat ttttatatat
    25981 caggtgtctg attttttgct ttagctctta aaatggtttg ccgcgaggtt ttgaattctt
    26041 gggcaatgtc acttatattt atacctgact taatttgctc tagtaccctc tgtatttgtt
    26101 catcatttaa cacaggtggg cgaccaaatc gctttcctgc gccccgtgct cttaatatcc
    26161 ctgaatgagt acgctcaagt aaaaggtctc gctcaaattc agcgactgct gaaattactt
    26221 gcatcatcat ttttccaggt ggactggtca ggtcaacgcc ccccaatgct aagcaatgca
    26281 ctttgatacc tgctttggtc agttgttcca ccgttttact gatatccatt ggattacaac
    26341 cgaggcgatc cagttttgtg acaatcaatg catcacctct tttcagtcga tcaagcaact
    26401 ggttaaaacc aggacgctca ctggttgctg ctgagccgct aatttgttct tcaattattt
    26461 gctgagattt gatgttaaaa cctgcacttt cgatttcccg acgttgattt ttggttgtct
    26521 tttccagagt tgatacccga cagtaagcat aaattcgaga catagtgaga tcttctata
//
LOCUS       DAZGHF010000064        26436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGHF010000064 DAZGHF010000000
VERSION     DAZGHF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.064"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            18..1487
                     /gene="gspA"
                     /locus_tag="ACHK3A_002728"
     CDS             18..1487
                     /gene="gspA"
                     /locus_tag="ACHK3A_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFO8740785.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSSQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLMEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTSVFT
                     AKHWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTDPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHQWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1489..1908
                     /gene="gspB"
                     /locus_tag="ACHK3A_002729"
     CDS             1489..1908
                     /gene="gspB"
                     /locus_tag="ACHK3A_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFO8740786.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2146..2457
                     /gene="rpsJ"
                     /locus_tag="ACHK3A_002730"
     CDS             2146..2457
                     /gene="rpsJ"
                     /locus_tag="ACHK3A_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO8740787.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2490..3119
                     /gene="rplC"
                     /locus_tag="ACHK3A_002731"
     CDS             2490..3119
                     /gene="rplC"
                     /locus_tag="ACHK3A_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO8740788.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3130..3735
                     /gene="rplD"
                     /locus_tag="ACHK3A_002732"
     CDS             3130..3735
                     /gene="rplD"
                     /locus_tag="ACHK3A_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO8740789.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3732..4034
                     /gene="rplW"
                     /locus_tag="ACHK3A_002733"
     CDS             3732..4034
                     /gene="rplW"
                     /locus_tag="ACHK3A_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO8740790.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4052..4873
                     /gene="rplB"
                     /locus_tag="ACHK3A_002734"
     CDS             4052..4873
                     /gene="rplB"
                     /locus_tag="ACHK3A_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO8740791.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4890..5168
                     /gene="rpsS"
                     /locus_tag="ACHK3A_002735"
     CDS             4890..5168
                     /gene="rpsS"
                     /locus_tag="ACHK3A_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO8740792.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5183..5515
                     /gene="rplV"
                     /locus_tag="ACHK3A_002736"
     CDS             5183..5515
                     /gene="rplV"
                     /locus_tag="ACHK3A_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO8740793.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5533..6234
                     /gene="rpsC"
                     /locus_tag="ACHK3A_002737"
     CDS             5533..6234
                     /gene="rpsC"
                     /locus_tag="ACHK3A_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO8740794.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6247..6657
                     /gene="rplP"
                     /locus_tag="ACHK3A_002738"
     CDS             6247..6657
                     /gene="rplP"
                     /locus_tag="ACHK3A_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO8740795.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6657..6848
                     /gene="rpmC"
                     /locus_tag="ACHK3A_002739"
     CDS             6657..6848
                     /gene="rpmC"
                     /locus_tag="ACHK3A_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO8740796.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6848..7102
                     /gene="rpsQ"
                     /locus_tag="ACHK3A_002740"
     CDS             6848..7102
                     /gene="rpsQ"
                     /locus_tag="ACHK3A_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO8740797.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7267..7638
                     /gene="rplN"
                     /locus_tag="ACHK3A_002741"
     CDS             7267..7638
                     /gene="rplN"
                     /locus_tag="ACHK3A_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO8740798.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7649..7963
                     /gene="rplX"
                     /locus_tag="ACHK3A_002742"
     CDS             7649..7963
                     /gene="rplX"
                     /locus_tag="ACHK3A_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO8740799.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7978..8517
                     /gene="rplE"
                     /locus_tag="ACHK3A_002743"
     CDS             7978..8517
                     /gene="rplE"
                     /locus_tag="ACHK3A_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO8740800.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8532..8837
                     /gene="rpsN"
                     /locus_tag="ACHK3A_002744"
     CDS             8532..8837
                     /gene="rpsN"
                     /locus_tag="ACHK3A_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO8740801.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8871..9263
                     /gene="rpsH"
                     /locus_tag="ACHK3A_002745"
     CDS             8871..9263
                     /gene="rpsH"
                     /locus_tag="ACHK3A_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO8740802.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9276..9809
                     /gene="rplF"
                     /locus_tag="ACHK3A_002746"
     CDS             9276..9809
                     /gene="rplF"
                     /locus_tag="ACHK3A_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO8740803.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9819..10172
                     /gene="rplR"
                     /locus_tag="ACHK3A_002747"
     CDS             9819..10172
                     /gene="rplR"
                     /locus_tag="ACHK3A_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO8740804.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10187..10690
                     /gene="rpsE"
                     /locus_tag="ACHK3A_002748"
     CDS             10187..10690
                     /gene="rpsE"
                     /locus_tag="ACHK3A_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO8740805.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10694..10873
                     /gene="rpmD"
                     /locus_tag="ACHK3A_002749"
     CDS             10694..10873
                     /gene="rpmD"
                     /locus_tag="ACHK3A_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO8740806.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10877..11311
                     /gene="rplO"
                     /locus_tag="ACHK3A_002750"
     CDS             10877..11311
                     /gene="rplO"
                     /locus_tag="ACHK3A_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO8740807.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11319..12650
                     /gene="secY"
                     /locus_tag="ACHK3A_002751"
     CDS             11319..12650
                     /gene="secY"
                     /locus_tag="ACHK3A_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO8740808.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12682..12798
                     /gene="rpmJ"
                     /locus_tag="ACHK3A_002752"
     CDS             12682..12798
                     /gene="rpmJ"
                     /locus_tag="ACHK3A_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO8740809.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12945..13301
                     /gene="rpsM"
                     /locus_tag="ACHK3A_002753"
     CDS             12945..13301
                     /gene="rpsM"
                     /locus_tag="ACHK3A_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO8740810.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13318..13707
                     /gene="rpsK"
                     /locus_tag="ACHK3A_002754"
     CDS             13318..13707
                     /gene="rpsK"
                     /locus_tag="ACHK3A_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO8740811.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13741..14361
                     /gene="rpsD"
                     /locus_tag="ACHK3A_002755"
     CDS             13741..14361
                     /gene="rpsD"
                     /locus_tag="ACHK3A_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO8740812.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14387..15376
                     /gene="rpoA"
                     /locus_tag="ACHK3A_002756"
     CDS             14387..15376
                     /gene="rpoA"
                     /locus_tag="ACHK3A_002756"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO8740813.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15417..15800
                     /gene="rplQ"
                     /locus_tag="ACHK3A_002757"
     CDS             15417..15800
                     /gene="rplQ"
                     /locus_tag="ACHK3A_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO8740814.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15907..16275
                     /gene="yhdN"
                     /locus_tag="ACHK3A_002758"
     CDS             15907..16275
                     /gene="yhdN"
                     /locus_tag="ACHK3A_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO8740815.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILADDSHVPPELR
                     AGYRLLKNAGCLLPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16286..16711
                     /gene="zntR"
                     /locus_tag="ACHK3A_002759"
     CDS             16286..16711
                     /gene="zntR"
                     /locus_tag="ACHK3A_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO8740816.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16767..16985
                     /gene="arfA"
                     /locus_tag="ACHK3A_002760"
     CDS             16767..16985
                     /gene="arfA"
                     /locus_tag="ACHK3A_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO8740817.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16982..17392)
                     /gene="mscL"
                     /locus_tag="ACHK3A_002761"
     CDS             complement(16982..17392)
                     /gene="mscL"
                     /locus_tag="ACHK3A_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO8740818.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPTPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17522..18898)
                     /gene="trkA"
                     /locus_tag="ACHK3A_002762"
     CDS             complement(17522..18898)
                     /gene="trkA"
                     /locus_tag="ACHK3A_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO8740819.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18920..20209)
                     /gene="rsmB"
                     /locus_tag="ACHK3A_002763"
     CDS             complement(18920..20209)
                     /gene="rsmB"
                     /locus_tag="ACHK3A_002763"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO8740820.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLNWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPPADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20255..21202)
                     /gene="fmt"
                     /locus_tag="ACHK3A_002764"
     CDS             complement(20255..21202)
                     /gene="fmt"
                     /locus_tag="ACHK3A_002764"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO8740821.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21217..21726)
                     /gene="def"
                     /locus_tag="ACHK3A_002765"
     CDS             complement(21217..21726)
                     /gene="def"
                     /locus_tag="ACHK3A_002765"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO8740822.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21856..22980
                     /gene="dprA"
                     /locus_tag="ACHK3A_002766"
     CDS             21856..22980
                     /gene="dprA"
                     /locus_tag="ACHK3A_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO8740823.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22952..23425
                     /gene="smg"
                     /locus_tag="ACHK3A_002767"
     CDS             22952..23425
                     /gene="smg"
                     /locus_tag="ACHK3A_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO8740824.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23454..23996
                     /gene="yrdD"
                     /locus_tag="ACHK3A_002768"
     CDS             23454..23996
                     /gene="yrdD"
                     /locus_tag="ACHK3A_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO8740825.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            24001..24573
                     /gene="tsaC"
                     /locus_tag="ACHK3A_002769"
     CDS             24001..24573
                     /gene="tsaC"
                     /locus_tag="ACHK3A_002769"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO8740826.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24578..25396
                     /gene="aroE"
                     /locus_tag="ACHK3A_002770"
     CDS             24578..25396
                     /gene="aroE"
                     /locus_tag="ACHK3A_002770"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO8740827.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25393..25650
                     /gene="yrdB"
                     /locus_tag="ACHK3A_002771"
     CDS             25393..25650
                     /gene="yrdB"
                     /locus_tag="ACHK3A_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO8740828.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25626..26180)
                     /gene="yrdA"
                     /locus_tag="ACHK3A_002772"
     CDS             complement(25626..26180)
                     /gene="yrdA"
                     /locus_tag="ACHK3A_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO8740829.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSAKNYVKWKDEYLDQGNQTQP"
     gene            complement(26224..>26436)
                     /locus_tag="ACHK3A_002773"
     CDS             complement(26224..>26436)
                     /locus_tag="ACHK3A_002773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740830.1"
                     /translation="EFQDFFSLLLRPSCEVFHVVVSTEAHYRDPYFLHKYFFDLFFCL
                     LLFHPFCCIRTQNGAFLHAKRLAQGQ"
BASE COUNT         6726 a         6252 c         6912 g         6546 t
ORIGIN      
        1 ttaaaacagg aagttctatg tctacgagaa gagaagttat tctttcctgg ttgcgtgaga
       61 aacgtcagac ctggcgtctg tgctatttgt tgggtgaggc tggaagtgga aaaacctggc
      121 tggcgcagca actacaaaaa gataaacatc gccgtgtgat tactttaagc ctcgttgttt
      181 cctcgcaagg taaggccgca tggatcgtta ccgacgataa tgcggctgaa cagggctgcc
      241 gtgacagcgc ctggacgcga gatgagatgg cgggtcaatt actgcatgcg cttcatcgaa
      301 ctgatagtcg ctgccctctt attattattg aaaatgctca cctgaatcac cgccgcatac
      361 ttgatgatct ccaacgcgcg atatctctta ttcctgacgg tcaatttttg ctgatcggca
      421 gacccgatcg aaaagtcgaa cgtgatttta aaaaacaggg cattgaactt gtctcaacag
      481 gacgtctgat ggagcacgag cttaaggcaa gcatccttga aggtcagaat attgaccagc
      541 ccgatctcct tttaactgcc agagttctga aacggatagc tttattatgt cggggagatc
      601 gcagaaagct ggcgcttgcc ggcgaaacaa ttagcttatt acagcaagcc gagcagacca
      661 gcgttttcac tgcaaaacat tggcgaatga tttaccgcgt attaggtgat aaccgccccc
      721 ggaaaatgca gcttgccgtg gtgatgagtg gaaccattat cgcacttacc tgtggttggc
      781 tactgctgtc ctctttcaca gatccgttcc ccgttcctgc gtggctggta cccgtaacgc
      841 cagtagttaa acaagatatg actaaggata ttgctcatgt agtgatgcgc gatagtgagg
      901 cgctaagcgt gttatacggc gtatgggggt acgaggtccc agctgattcg gcctggtgcg
      961 atcaggcagt aagagcgggg ctggcatgta aaagtgggaa tgcttcttta caaacattgg
     1021 tagatcagaa tttgccctgg atcgcatcgc ttaaagtcgg ggacaaaaaa ttgcctgttg
     1081 tggtcgttcg cgtcggtgat gctaccgttg atgtcctcgt agggcagcaa acctggacgc
     1141 tgacacatca atggtttgag tcggtatgga cgggtgatta tcttctgtta tggaaaatgt
     1201 cgccggaagg ggagagtacc attacgcgtg atagcagcga ggaagagatt ctctggctgg
     1261 aaacgatgtt aaaccgcgca ttgcatattt cgacagaacc ttcggcagag tggcgtccct
     1321 tgttggtaga aaaaataaag caatttcaaa aaagccacca cttgaaaact gacggtgttg
     1381 ttggcttttc aacgttagta catctctggc aggtggcagg ggagagtgcc tacttatatc
     1441 gggatgaagc aaatatttcc ccggaaacaa cagtgaaggg gaaatgacat gtttgaattc
     1501 tatatcgccg cgcgtgagca gaaagaaaca ggtcaccctg gtatcttctc ccgacaaaaa
     1561 cacagcacca taatatatgt catttgttta ttgctgattt gcctgtggtt tgcaggaatg
     1621 gtgttggttg gtgggtatgc caggcagcta tgggcgcttt ggatagtaaa agcagaagtc
     1681 actgtagagg ctgaaacgcc cgcttttaaa caatcaacac aacactattt cttcaaaaaa
     1741 cagccactcc ccgtcgtcga atccgtcgag gaggaagatg acccagacgt cgcggttgag
     1801 aatgctccct cttcttctga agacgaagaa aatactgtag aagaaagcga cgagaaagcc
     1861 ggtttaagag agcgtgtcaa aaatgcgctg aacgaacttg agagataacc cgaaggctgt
     1921 ttactttact agcaatacgc ttgcgttcgg tggttaagta tgtataatgc gcgggcttgt
     1981 cgtagttgac agcaggttca atctgaacct cagttagccg aatttggcta cctaacaatg
     2041 ctcccaatcg gggagctacg taagaacggt tacactctcc catcaatcgt aatgggtctg
     2101 aggagtaatc attttcgttt ataaaataat tggagctctg gtctcatgca gaaccaaaga
     2161 atccgtatcc gcctgaaagc gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc
     2221 gtcgagactg ccaagcgcac tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc
     2281 aaagagcgct tcactgttct gatctccccg cacgtcaaca aagacgcgcg cgatcagtac
     2341 gaaatccgta ctcacttgcg tctggttgac atcgttgagc caaccgagaa aaccgttgat
     2401 gctctgatgc gtctggatct ggctgccggt gtagacgtgc agatcagcct gggttaatca
     2461 ggtcattgag cgattgagag gttgaaacaa tgattggttt agtcggtaaa aaagtgggta
     2521 tgacccgtat cttcacagaa gacggcgttt ctatcccagt aaccgtaatc gaagttgaag
     2581 caaaccgcgt tactcaggtt aaagacctgg ctaacgatgg ctaccgtgct attcaggtaa
     2641 ccaccggtgc taaaaaagct aaccgtgtga ccaagcctga agctggccac ttcgctaaag
     2701 ctggcgtaga agctggccgt ggtctgtggg aattccgcct ggctgaaggc gaagagttca
     2761 ctgtaggtca gagcattagc gttgaactgt ttgctgacgt taaaaaagtt gacgtaactg
     2821 gcacctctaa aggtaaaggt ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg
     2881 acgctactca cggtaactcc ttgtctcacc gcgttccggg ttctatcggt cagaaccaga
     2941 ctccgggcaa agtgttcaaa ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa
     3001 ccgttcagag ccttgacgta gtacgcgttg acgctgagcg caacctgctg ctggttaaag
     3061 gtgctgtccc gggtgcaacc ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag
     3121 gagatagcaa tggaattagt attgaaagac gcgcagagcg cgctgactgt ttccgaaact
     3181 accttcggtc gtgatttcaa cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct
     3241 ggtgctcgtc agggtactcg tgctcagaag actcgtgctg aagtaactgg ttccggtaaa
     3301 aaaccgtggc gccagaaagg caccggccgt gcgcgttctg gttctatcaa gagcccgatc
     3361 tggcgttctg gtggcgtgac ctttgctgct cgtccgcagg accacagtca aaaagttaac
     3421 aagaagatgt accgcggcgc gctgaaaagc atcctgtccg aactggtacg tcaggatcgt
     3481 ctgatcgttg tcgagaagtt ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa
     3541 ctgaaagaca tggctctgga agatgtgctg atcatcaccg gtgagctgga cgaaaacctg
     3601 ttcctggctg cgcgcaacct gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg
     3661 gttagcctga tcgccttcga caaagtcgta atgactgctg atgctgttaa gcaagttgag
     3721 gagatgctgg catgattcgt gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt
     3781 ctgaaaaagc gtctactgcg atggaaaaat ctaacaccat cgtactcaaa gttgctaaag
     3841 acgcgaccaa agcagaaatc aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag
     3901 tcgttaacac cctggtagtt aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc
     3961 gtagcgactg gaaaaaagct tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg
     4021 gcggcgctga gtaagtcgga ggagtaatac aatggcagtt gttaaatgta aaccgacatc
     4081 tccgggtcgt cgccacgttg ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt
     4141 tgctccgttg ctggaaaaaa acagcaaatc cggtggtcgt aacaacaatg gccgtatcac
     4201 cactcgtcat atcggtggtg gccacaagca ggcttaccgt attgttgact tcaaacgcaa
     4261 caaagacggt atcccggcag ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa
     4321 catcgcgctg gttttgtaca aagacggtga acgccgttac atcctggccc ctaaaggcct
     4381 gaaagctggc gaccagattc agtctggcgt tgatgctgca atcaaaccag gtaacaccct
     4441 gccgatgcgc aacatcccgg ttggttctac tgttcataac gtagaaatga aaccaggtaa
     4501 aggcggtcag ctggcacgtt ccgctggtac ttacgttcag atcgttgctc gtgatggtgc
     4561 ttatgtcacc ctgcgtctgc gttctggtga aatgcgtaaa gtagaagcag actgccgtgc
     4621 aactctgggc gaagttggca atgctgagca tatgctgcgc gttctgggta aagcaggtgc
     4681 tgcacgctgg cgtggtgttc gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca
     4741 cccacatggt ggtggtgaag gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt
     4801 tcagaccaaa ggtaagaaga cccgcagcaa caagcgtact gataaattca tcgtacgtcg
     4861 ccgtagcaaa taattttaga ggataagcca tgccacgttc tctcaagaaa ggtcctttta
     4921 ttgacctgca cttgctgaag aaggtagaga aagcggtgga aagcggagac aagaagcccc
     4981 tgcgcacttg gtcccgtcgt tcaacgatct ttcctaacat gatcggtttg accatcgctg
     5041 tccataatgg tcgtcagcac gttccggtat ttgtaaccga cgaaatggtc ggtcacaaac
     5101 tgggtgaatt cgcaccgact cgtacttatc gcggccacgc tgctgataaa aaagcgaaga
     5161 agaaataagg taggaggaag agatggaaac tatcgctaaa catcgccatg ctcgttcttc
     5221 tgctcagaag gttcgccttg ttgctgacct gattcgcggt aagaaagtgt cgcaggctct
     5281 ggatattttg acctacacca acaagaaagc ggctgtactg gtcaagaaag ttctggaatc
     5341 tgccattgct aacgctgaac acaacgatgg cgctgacatt gacgatctga aagttacgaa
     5401 aattttcgta gacgaaggcc cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc
     5461 agatcgcatc ctgaagcgca ccagccacat cactgtggtt gtgtccgatc gctgagactc
     5521 tggagactag caatgggtca gaaagtacat cctaatggta ttcgcctggg tattgtaaaa
     5581 ccatggaact ctacttggtt tgcgaacacc aaagaattcg ctgacaacct ggacagcgat
     5641 tttaaagtac gtcagtacct gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt
     5701 atcgagcgtc cggctaagag catccgtgta accattcaca ctgctcgccc gggtatcgtt
     5761 atcggtaaaa aaggtgaaga cgtagaaaaa ctgcgtaagg tcgtagcgga catcgctggc
     5821 gttcctgcac agatcaacat cgccgaagtt cgtaagcctg aactggacgc aaaactggtt
     5881 gctgacagca tcacttctca gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt
     5941 gctgtacaga acgcaatgcg tctgggcgct aaaggtatta aagttgaagt tagcggccgt
     6001 ctgggcggcg cagaaatcgc acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac
     6061 actctgcgtg ctgacatcga ctacaacacc tctgaagcgc acaccactta cggtgtaatc
     6121 ggcgttaaag tgtggatctt caaaggcgag atcctgggtg gtatggctgc tgttgaacaa
     6181 ccggaaaaac cggctgctca gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg
     6241 tcgctgatgt tacaaccaaa gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc
     6301 ggtctggcgc agggtacgga tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt
     6361 ggtcgtctga ctgcccgtca gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag
     6421 cgtcaaggta agatctggat ccgtgtgttc ccggacaaac cgatcactga aaagccgctg
     6481 gcagtgcgta tgggtaaagg taaaggtaac gtggagtatt gggttgcctt gattcagccg
     6541 ggtaaagtcc tgtatgaaat ggacggtgtt ccggaagagc tggcccgtga agcattcaag
     6601 ctggcagcag cgaaactgcc gattaaaacc acctttgtaa ctaagacggt gatgtaatga
     6661 aagcaaaaga gctgcgtgag aagagcgttg aagagctgaa caccgagctg ctgaacctgc
     6721 tgcgtgagca gttcaacctg cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc
     6781 tgttgaagca agtgcgtcgc gatgtcgcac gcgttaagac tttactgaac gagaaggcgg
     6841 gtgcgtaatg accgataaaa tccgtactct gcaaggtcgc gttgttagcg acaaaatgga
     6901 gaaatccatt gttgttgcta tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat
     6961 caagcgtacg accaaactgc acgtacatga cgagaacaac gaatgcggta tcggtgacgt
     7021 ggttgaaatc cgcgaatgcc gtccgctgtc caagactaaa tcctggacgc tggttcgcgt
     7081 tgtagagaaa gcggttctgt aatacagtac actctctcga tacgaataaa cggctcagaa
     7141 atgagccgtt tattttttct acccatatcc ttgaagcggt gttataatgc cgcgccctcg
     7201 atatggggat ttttaacgac ctgattttcg ggtctcagta gtagttgaca ttagcggagc
     7261 actaaaatga tccaagaaca gactatgctg aacgtcgccg acaactccgg tgcacgtcgc
     7321 gtaatgtgta tcaaggttct gggtggctcg caccgtcgct acgcaggcgt aggcgacatc
     7381 atcaagatca ccatcaaaga agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg
     7441 aaggcggtag tggtgcgcac caagaagggt gttcgtcgcc cggacggttc tgtcattcgc
     7501 ttcgatggta atgcttgtgt tcttctgaac aacaacagcg agcagcctat cggtacgcgt
     7561 atttttgggc cggtaactcg tgagcttcgt agtgagaagt tcatgaaaat tatctctctg
     7621 gcaccagaag tactctaagg agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt
     7681 tatcgtgtta accggtaaag ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc
     7741 cggcaaggtc attgttgaag gtatcaacct ggttaagaaa catcagaagc cggttccggc
     7801 cctgaaccaa ccgggtggca tcgttgaaaa agaagccgct attcaggttt ccaacgtagc
     7861 aatcttcaat gcggcaaccg gcaaggctga ccgtgtaggc tttagattcg aagacggtaa
     7921 aaaagtccgt ttcttcaagt ctaacagcga aactatcaag taatttggag tagtacgatg
     7981 gcgaaactgc atgattacta caaagacgaa gtagttaaaa aactcatgac tgagtttaac
     8041 tacaattctg tcatgcaagt ccctcgggtc gagaagatca ccctgaacat gggtgttggt
     8101 gaagcgatcg ctgacaaaaa actgctggat aacgcagcag cagacctggc agcaatctcc
     8161 ggtcaaaaac cgctgatcac caaagcacgc aaatctgttg caggcttcaa aatccgtcag
     8221 ggctatccga tcggctgtaa agtaactctg cgtggcgaac gcatgtggga gttctttgag
     8281 cgcctgatca ctattgctgt acctcgtatc cgtgacttcc gtggcctgtc cgctaagtct
     8341 ttcgacggtc gtggtaacta cagcatgggt gtccgtgagc agatcatctt cccagaaatc
     8401 gactacgata aagtcgaccg cgttcgtggt ttggatatta ccattaccac tactgcgaaa
     8461 tctgacgaag aaggccgcgc tctgctggct gcctttgact tcccgttccg caagtaaggt
     8521 agggttacta aatggctaag caatcaatga aagcacgcga agtaaaacgc gtagctttag
     8581 ctgataaata cttcgcgaaa cgcgctgaac tgaaagcgat catctctgat gtgaacgctt
     8641 ccgacgaaga tcgttggaac gctgttctca agctgcagac tctgccgcgt gattccagcc
     8701 cgtctcgtca gcgtaaccgc tgccgtcaaa caggtcgtcc gcatggtttc ctgcggaagt
     8761 tcgggttgag ccgtattaag gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga
     8821 aaaaggctag ctggtaattg tcaccaattg aatcacggga gtaaagacag atgagcatgc
     8881 aagatccgat cgcggatatg ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg
     8941 cggtcaccat gccttcctcc aagctgaaag tggcaatcgc caacgtgctg aaggaagaag
     9001 gttttattga agattttaaa gttgaaggcg acaccaagcc tgaactggaa cttactctga
     9061 agtatttcca gggcaaagct gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc
     9121 gcatctataa gcgtaaagat gagctgccga aagttatggc gggtctgggt atcgcagttg
     9181 tttctacctc taaaggtgtt atgactgatc gtgcagcgcg ccaggctggt cttggtggcg
     9241 aaattatctg ctacgtagcc taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg
     9301 tcgttgttcc tgccggcgtt gacgtaaaaa tcaacggtca ggttattacg atcaaaggta
     9361 aaaacggcga gctgactcgt actctcaacg atgctgttga agttaaacat gcagataata
     9421 ccctgacctt cggtccgcgt gatggttacg cagacggttg ggcacaggct ggtaccgcgc
     9481 gtgccctgct gaactcaatg gttatcggtg ttaccgaagg cttcactaag aagctgcagc
     9541 tggttggtgt aggttaccgt gcagcggtta aaggcaatgt gattaacctg tctctgggtt
     9601 tctctcatcc tgttgaccat cagctgcctg cgggtatcac tgctgaatgt ccgactcaga
     9661 ctgaaatcgt gctgaaaggc gctgataagc aggtgatcgg ccaggttgca gcggatctgc
     9721 gcgcctaccg tcgtcctgag ccttacaaag gcaagggtgt tcgttacgcc gacgaagtcg
     9781 tgcgtaccaa agaggctaag aagaagtaag gtaacactat ggataagaaa tctgctcgta
     9841 tccgtcgtgc gacccgcgca cgccgcaagc tccaggagct gggcgcaact cgcctggtgg
     9901 tacatcgtac cccgcgtcac atttacgcac aggtaattgc accgaacggt tctgaagttc
     9961 tggtagctgc ttctactgta gaaaaagcta tcgctgaaca actgaagtac accggtaaca
    10021 aagacgcggc tgcagctgtg ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca
    10081 aagatgtatc ctttgaccgt tccgggttcc aatatcatgg tcgtgtccag gcactggcag
    10141 atgctgcccg tgaagctggc cttcagttct aaggtagagg tgtaagatgg ctcacatcga
    10201 aaaacaagct ggcgaactgc aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt
    10261 taaaggtggt cgtattttct ccttcacagc tctgactgta gttggcgatg gtaacggtcg
    10321 cgttggtttt ggttacggta aagcgcgtga agttccagca gcgatccaga aagcgatgga
    10381 aaaagcccgt cgcaatatga ttaacgtcgc gctgaataac ggtactctgc aacaccctgt
    10441 taaaggtgtt catacgggtt ctcgcgtatt catgcagccg gcttccgaag gtaccggtat
    10501 catcgccggt ggtgcaatgc gcgccgttct ggaagtcgct ggggttcata acgttctggc
    10561 taaagcgtat ggttccacca acccgattaa cgtggttcgt gcaactatcg atggcctgga
    10621 aaatatgaat tccccagaaa tggtcgctgc caagcgtggt aaatccgttg aagaaattct
    10681 ggggaaataa accatggcaa agactattaa aattactcaa acccgcagtg caatcggtcg
    10741 tctgccgaaa cacaaggcaa cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt
    10801 agagcgcgag gatactcctg ctattcgcgg tatgatcaac gcggtttcct tcatggttaa
    10861 agttgaggag taagagatgc gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc
    10921 gggtaaacgc ctgggtcgtg gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca
    10981 caaaggtcag aaatctcgtt ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat
    11041 gcctctgtac cgtcgtctgc cgaaattcgg cttcacttct cgtaaagcag cgattacagc
    11101 cgaagttcgt ctgtctgacc tggctaaagt agaaggcggt gttgtagacc tgaacacgct
    11161 gaaagcggct aacattatcg gtatccagat cgagttcgcg aaagtgatcc tggctggcga
    11221 agtcactact ccggtaactg ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat
    11281 cgaagctgct ggcggtaaaa tcgaggaata agtagcagat ggctaaacaa ccgggattag
    11341 attttcaaag tgccaaaggt ggcttaggcg agctgaaacg cagactgctg tttgttatcg
    11401 gtgcgctgat tgtgttccgt attggctctt ttattccgat ccctggtatt gatgccgctg
    11461 tacttgccaa actgcttgag caacagcgag gcaccatcat tgaaatgttt aacatgttct
    11521 ctggtggtgc tctcagccgt gcttctatct ttgctctggg gatcatgcca tatatttcgg
    11581 catcgatcat tatccagctt ctgacggtgg ttcacccaac gttggcagaa attaagaaag
    11641 aaggggagtc tggtcgtcgt aagatcagcc agtacacccg ctacggtact ctggtgctgg
    11701 caatattcca gtcgatcggt attgctaccg gtctgccgaa tatgcctggt atgcaaggcc
    11761 tggtcattaa cccgggcttt gcattctact tcaccgctgt tgtaagtctg gtcacaggaa
    11821 ccatgttcct gatgtggttg ggcgaacaga ttactgaacg aggtatcggc aacggtattt
    11881 caatcattat cttcgccggt attgtcgcgg gactcccgcc agccattgcc catactatcg
    11941 agcaagcgcg tcaaggcgac ctgcacttcc tcgtgttgct gttggttgca gtattagtat
    12001 ttgcagtgac gttctttgtt gtatttgttg agcgtggtca acgccgcatt gtggtaaact
    12061 acgcgaaacg tcagcaaggt cgtcgtgtct atgctgcaca gagcacacat ttaccgctga
    12121 aagtgaatat ggcgggggta atcccggcaa tcttcgcttc cagtattatt ctgttcccgg
    12181 cgaccatcgc gtcatggttc gggggcggta ctggttggaa ctggctgaca acaatttcgc
    12241 tgtatttgca gcctgggcaa ccgctttatg tgttactcta tgcgtctgca atcatcttct
    12301 tctgtttctt ctacacggcg ttggttttca acccgcgtga aacagcagat aacctgaaga
    12361 agtccggtgc atttgtacca ggaattcgtc cgggagagca aacggcgaag tatatcgata
    12421 aagtaatgac ccgcctgacc ctggttggtg cgctgtacat tacctttatc tgcctgatcc
    12481 cggagttcat gcgtgatgca atgaaagtac cgttctactt tggtgggacc tcactgctta
    12541 tcgttgttgt cgtgattatg gactttatgg ctcaagtgca aactctgatg atgtccagtc
    12601 agtatgagtc tgcattgaag aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc
    12661 gagaagttac ggagagtaaa aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac
    12721 tgcaaaatcg ttaagcgtga tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat
    12781 aaacagcgcc aaggctgatt ttttcgcata tttttcttgc aaagttgggt tgagctggct
    12841 agattagcca gccaatcttt tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg
    12901 gcttttcagc atggaacgta catattaaat agtaggagtg catagtggcc cgtatagcag
    12961 gcattaacat tcctgatcat aagcatgccg taatcgcatt aacttcgatt tatggcgtcg
    13021 gcaagacccg ttctaaagcc atcctggctg cagcgggtat cgctgaagat gttaagatca
    13081 gtgagctgtc tgaaggacaa atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg
    13141 aaggtgatct gcgccgtgaa atcagcatga gcatcaagcg cctgatggat cttggttgct
    13201 atcgcggttt gcgtcatcgt cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg
    13261 cacgtacccg taagggtccg cgcaaaccga tcaagaaata atcggggtga ttgaataatg
    13321 gcaaaggcac caattcgtgc acgtaaacgt gtaagaaaac aagtctctga cggcgtggct
    13381 catatccatg cttctttcaa caacaccatc gtgactatca ctgatcgtca gggtaacgcg
    13441 ttgggttggg caacagccgg tggttccggt ttccgtggtt ctcgcaaatc cactccgttt
    13501 gcagctcagg ttgcagcaga gcgttgcgct gacgccgtga aagaatacgg catcaagaat
    13561 ctggaagtta tggttaaagg tccgggtcca ggccgcgaat ctactattcg tgctctgaac
    13621 gccgcaggtt tccgcatcac taacattact gatgtgactc cgatccctca taacggttgt
    13681 cgtccgccga aaaaacgtcg cgtataacgc ttcgttttcc aggtttgttg gagaaagaaa
    13741 atggcaagat atttgggtcc taagctcaag ctgagccgtc gtgagggcac cgacttattc
    13801 cttaagtctg gcgttcgcgc gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag
    13861 cacggtgcgc gtaaaccgcg tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa
    13921 gttcgccgta tctatggtgt gctggagcgt cagttccgta actactacaa agaagcagca
    13981 cgtctgaaag gcaacaccgg tgaaaacctg ttggctctgc tggaaggtcg tctggacaac
    14041 gttgtatacc gtatgggctt cggtgccact cgtgcagaag cacgtcagct ggttagccat
    14101 aaagcaatta tggtaaacgg tcgtgttgtt aacattgctt cttatcaggt tagtccgaat
    14161 gacgttgtaa gcattcgtga gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag
    14221 ctggctgagc agcgtgaaaa gccaacctgg ctggaagttg atgctggcaa gatggaaggt
    14281 acgtttaagc gtaagccgga gcgttctgat ctgtctgcgg acattaacga acacctgatc
    14341 gtcgagcttt actccaagta aagcttagta ccaaagagag gacacaatgc agggttctgt
    14401 gacagagttt ctaaaaccgc gcctggttga tatcgagcaa gtgagttcga cgcacgccaa
    14461 ggtgaccctt gagcctttag agcgtggctt tggccatact ctgggtaacg cactgcgccg
    14521 tattctgctc tcatcgatgc cgggttgcgc ggtgaccgag gttgagattg atggtgtact
    14581 acatgagtac agcaccaaag aaggcgttca ggaagatatc ctggaaatcc tgctcaacct
    14641 gaaagggctg gcggtgagag ttcagggcaa agatgaagtt attcttacct tgaataaatc
    14701 tggcattggc cctgtgactg cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa
    14761 gccgcagcac gtgatctgcc acctgaccga tgagaacgcg tctattagca tgcgtatcaa
    14821 agttcagcgc ggtcgtggtt atgtgccggc ttctacccga attcattcgg aagaagatga
    14881 gcgcccaatc ggccgtctgc tggtcgacgc atgctacagc cctgtggagc gtattgccta
    14941 caatgttgaa gcagcgcgtg tagaacagcg taccgacctg gacaagctgg tcatcgaaat
    15001 ggaaaccaac ggcacaatcg atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc
    15061 tgaacaactg gaagctttcg ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga
    15121 gaaaccagag ttcgatccga tcctgctgcg ccctgttgac gatctggaat tgactgtccg
    15181 ctctgctaac tgccttaaag cagaagctat ccactatatc ggtgatctgg tacagcgtac
    15241 cgaggttgag ctccttaaaa cgcctaacct tggtaaaaaa tctcttactg agattaaaga
    15301 cgtgctggct tcccgtggac tgtctctggg catgcgcctg gaaaactggc caccggcaag
    15361 catcgctgac gagtaaccgg atcacaggtt aaggttttac tgagaaggat aaggtcatgc
    15421 gccatcgtaa gagtggtcgt caactgaacc gcaacagcag ccatcgccag gctatgttcc
    15481 gcaatatggc aggttcactg gttcgtcatg aaatcatcaa gacgactctg cctaaagcga
    15541 aagagctgcg ccgcgtagtt gagccgctga ttactcttgc caagactgat agcgttgcta
    15601 atcgtcgtct ggcattcgcc cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg
    15661 aactgggccc gcgtttcgcg agccgtgccg gtggttacac tcgtattctg aagtgtggct
    15721 tccgtgcagg cgacaacgcg ccgatggctt acatcgagct ggttgatcgt tcagagaaag
    15781 cagaagctgc tgcagagtaa tctgaagcaa cgtaaaaaaa cccgccccgg cgggtttttt
    15841 tatacccgta gcatccccac ttatctacaa tagctgtact ctttttgttc atcccctgga
    15901 gtatatatgt ggttacttga ccagtgggca gagcgtcata tagcagaagc gcaagcgaaa
    15961 ggtgagtttg ataacctggc aggtagcggc gaaccattga tactggctga tgattctcat
    16021 gtgccaccgg aattacgtgc ggggtatcgc ttgctgaaga atgccggttg cttactgcca
    16081 gaacttgagc agcggaggga agcaattcag cttctggata tcctgagagg tatccgtcac
    16141 gatgatccgc aatatcaaga ggttagccgt cgattgtcat tactggaatt gaagctgcga
    16201 caagctggac tgagcaccga ttttttacgc ggcgattatg ctgacaagtt gttgaataaa
    16261 atcaacgata actagtggag tatgtatgta tcgcattggt gagctggcaa aaatggcgga
    16321 agtaacaccc gacacgattc gttattacga aaaacagcag atgatggagc atgaagtgcg
    16381 tactgaaggt gggtttcgcc tgtataccga aagcgatctc cagcgattga aatttatccg
    16441 ccatgccaga caactaggtt tcagtctaga gtcgatccgc gagttgctgt cgatccgtat
    16501 cgatcctgaa caccatacct gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga
    16561 agtcgaagca cggatagccg agttgcagag tatgcagcgt tccctccaac gccttaacga
    16621 tgcctgttgt gggaccgccc atagcagtgt ttattgctcg attcttgaag ctcttgaaca
    16681 aggggcgagt ggcgttaaga gtggctgttg attttttaca ctggcaggat tacactcgcg
    16741 ccgttaaata accaattgga gtttttatga gccgatatca gcatactaaa gggcagataa
    16801 aggataatgc gatagaagca ttactacatg atcccttatt ccgacagcgc gtagagagaa
    16861 ataagaaagg gaaaggcagt tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg
    16921 aggccagtgg caagaaagta aatcactttt ttaccactgg ccttctgctt tcaggcgctt
    16981 gttaagagcg gttattctgc tctttcagca aatcacgaat ttctgtcagt aatacttctt
    17041 ctttagttgg tgtaggcgcg gctgctggtt cttctttttt ccgattcagt ttgttgatta
    17101 gcttaatcgc cataaagatg gcaaaggcta caatcagaaa atcaaagacg ttttgaatga
    17161 agacaccgta atgcatcaca acagcaggga tatccccctg cgcatcgcgt agcgtgacag
    17221 caaactgttt gaaatcgatc ccgccaatta ataaccccag aggtggcata atgatatcgg
    17281 caaccagtga agatacaatc ttcccgaatg ccgcaccgat aatgacaccc accgccaaat
    17341 ccaccacgtt cccgcgcatc gcaaattcgc gaaattcttt aataatgctc atgttattct
    17401 ccctatgaag ccgacaatca taagtctaac aaatgttaag ctattttcct gccaggagtg
    17461 aatttaatta atccctgata atcaaaggga aataataagg cgtcattaga cgccttatta
    17521 attacaagaa gaaaggactt ggctggaaga gtctttcgac gtcggtaata aactttttat
    17581 ctgtgaggaa cataattacg tgatcgcctt gctcaatgcg cagattgtca ttggcaatca
    17641 tcacgtcgtt tccacgtacc accgctccaa taatcgttcc tggcggtagc ttgatttcat
    17701 caatgactct gccgacaacg cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag
    17761 cttctgctac gccgcggcgc agtgaggaaa caccaacaat atcagctttt cgcacatggc
    17821 taagcaacgc agaaatagtt gcttgttgcg gtgaaatcgc aatatcgatc acgctcccct
    17881 gaaccagatc cacataagcg cgacgctgga tcaataccat cacctttttc gcacccatac
    17941 gtttggcaag catggcggac atgatattgg cttcgtcatc gttggtgaca gcaataaaca
    18001 gatcaacttg atcgatatgt tcttcggcca gcagttcttg atccgacgca tcaccaaaaa
    18061 agacgatcgt attctgcaac ttttccgcca gttcggcagc gcgctgctga ttacgttcga
    18121 tgagtttaac gctgtaatct ttttccagac gacgcgccag ccctgcaccg atattaccgc
    18181 cgccaaccag catgatccgc ttatacggtt tttccagtcg ctgtaattca ctcatcaccg
    18241 cgcggatatg ctgtgaagcg gcaataaaga acacttcatc accagcttca acaatggtcg
    18301 aaccttgcgg acgaatgggg cgatcgtggc ggaaaattgc tgccacacga gtatcgatat
    18361 ggggcatatg ttcgcgcatg gtcgaaagtg cattaccaat cagcgggccg ccataatagg
    18421 ctttaaccac agccaggctg actttaccct cagcgaagtt caccacctgc aatgcgccgg
    18481 gatactcaat cagtcggtaa atattatcga taaccaactg ctctggtgcg atcagatgat
    18541 caatcggtac agcatctgaa tgaaatagtt tatcggcatc gcgcacgtag tctggtgagc
    18601 ggatacgagc gatgcgatta ggggtgttga aaagtgagta ggctacctgg caggcaacca
    18661 tattggtttc atctgaacta gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct
    18721 cccgcaatac gcgtggatga gagccatgcc cctgcacgac tcgcaggtca aatttatcct
    18781 gcaaggtccg cagacgctca ccgttggtat cgacaacagt aatatcgttg ttctcgccaa
    18841 ccaggttttc cgccagtgtg ccgccaacct ggccggcacc cagaatgata attttcatca
    18901 gtcgcgaccc gttatctcat cactttttga ttagcttagc gtaaaagaag ccgtcgccct
    18961 cttcggcacc aggcagattt tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt
    19021 cggcatcagc ggtacgttgc aaaaaggctt taatctgcag gctattctct tccggtaaca
    19081 ccgaacaggt ggcatagacc agggttccac cggattttaa atgcggccaa atggcgtcga
    19141 gaatttcaga ctgcaattgc gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa
    19201 tatccggatg gcgacgaatc acaccggttg ctgaacaagg cgcatctaat aaaatgcgat
    19261 caaactgttg ctcgccacac cattgggaag ggtagcggcc atcaccttgt ttcacggttg
    19321 ccttcatacc aaggcgtttt aaattgtcgt aaacgcgaga gaggcgctgt tcgtcaatat
    19381 caaccgcaac aacctgcgct tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg
    19441 gggcggcaca aagatccaaa atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac
    19501 cttgtgctga ggcatcctga acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa
    19561 caggtgcagg tgtttccaga cgtacagcat cagggtaatc cgcaggcggg aaacctttca
    19621 ttcctgcttc atccagcaat gcaagccagc tgtcgcggga atgatgcgta cgattaacgc
    19681 gcagccacat tggcggacgc tggttattgg cttcgacgat ggattgccac tgctctggat
    19741 acgctttttg cagacgcttc agcaaccagg aaggatgcag ataacgtgca tcactggcat
    19801 taaactcggc taataactct tcttgctgac gctggaactg gcgtaataca ccgtttatca
    19861 accctttaag ttgcgggcgc ttaattgcga tagcgccttc aaccgtttca gccagcgcag
    19921 catgaggtgg tatgcgggta taaagcagtt gatacaaacc aaccataatc aggtaatgca
    19981 cggtccgctg tttgccggtc atcggacggg ccattaactt attaatcagc cagtttaact
    20041 gcgaaagcgt acgcagtacg ccaaagcaca actcttgaag aagtgctttg tctttatcgg
    20101 aaactttttg ctggagcggt ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt
    20161 gttcaacggc ctgggccgcc atgctacgta aattacgttg ttttttcata agtataaaaa
    20221 tgcccggcaa gaccgggctt agaagagtgg actatcagac cagacggttg cccggaacaa
    20281 accattcccg acgagagttc aggaggtctt gcgcgctcat cgctttctta cccgcaggtt
    20341 gtaacgagag caggttcagg atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg
    20401 cttcaaggat cgttcctggt gcagcgttgg ttgccgtatc aatgaccgat gctttccaga
    20461 ctttaaccgg ctgtccttca atttccagcc agctcattgg ccatggattg aaagcgcgaa
    20521 tgcagcgttc aagctgtgct gccgaaagtg accagtcaat acgcgcttct tctttactca
    20581 acttctcggc gtaagtgaca agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg
    20641 ccaattgttt caacgtggtg ataagccctt gtgggccaag ctctgccagc ttgtcgtaca
    20701 gcgtaccact ggtatcttct gcagtaatcg ggcaggagag cttatagagc atgtctccgg
    20761 tgtctaaacc aacatccatt tgcataatgg tcacaccagt ttctgcatca cccgcccata
    20821 gtgagcgttg gattggtgca gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga
    20881 tacagccaag acgcggcatc tccagcactg ctttaggcag aattaagcca taggcgacga
    20941 caaccataac atcagcctgt agatcggcga ccagttgctg gttttcttgt ggacgcaggg
    21001 aaaccggttg aaaaacgggc agaccttttt cctcagccag aactttaacc gggctgggca
    21061 tcagtttttt accgcgtcct gccggtcggt ctggctgggt gaacacgcca acgacgttat
    21121 gaccagaaga caacagcgcg tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa
    21181 taatacgtag tgattctgac acgttagttc ttatccttaa gcccgggctt tcagacgatc
    21241 cagtttttca actttctgac gaatacgttg ttgtttcagc ggtgacagat aatccataaa
    21301 cagtttgccg accaggtgat ccatctcatg ctgaatacag atggctaaca gaccctctgc
    21361 ttccagttca aatggtttac cgtcgcggtc cagggcgcga attttaactt tctctgcgcg
    21421 tggcactaaa gcacgttgtt cagggatcga caggcatcct tcttcaatgc ctgtttcgcc
    21481 gcttttttct aaaagctccg gattgattaa caccagccgt tcgtcacggt tttccgaaac
    21541 atcaataaca atgatacgtt gatggatatc gacctgggtt gccgccaggc caataccttc
    21601 ttctgcgtac atcgtctcga acatatcatc gacgatacgc tgaatttctg cattcacttc
    21661 ttctaccggt ttagcaactt tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac
    21721 tgacataaat ctccagagat gtgttcagga gttagaaaga ttatttcttc tattctagac
    21781 aaatccccct ctgattgaca gcatcactga ccaatcgcaa agattgctaa ggctgcttat
    21841 ggcagggaga taaggatggt cgatacagaa atttggctgc gtttaatgag catcagcagc
    21901 ttgtacggcg atgatatggt ccgtatagct cactggctgg caaaacagtc gcatattgat
    21961 gcggttgtat tgcagcaaac tgggcttaca ttgcggcagg cacaacgctt tctttcattt
    22021 ccgcgaaaga gtatcgaaag ctcactttct tggttggagc aacccaacca tcatttaatt
    22081 cctgcggaca gcgaatttta tcctcctcaa cttcttgcga cgacagatta ccccggcgca
    22141 ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt agtggggagt
    22201 cgggcgcatt catggtatgg cgagcgatgg ggacgattat tttgcgaaac tctggcgacg
    22261 cgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggc gcataaagcg
    22321 gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa taccattcat
    22381 ccgcgtcgcc atgcccgact ggctaccagt ttgcttgaac atggtggggc tcttgtctcg
    22441 gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa tcgcattatc
    22501 agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg
    22561 acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccggg tccattaggg
    22621 aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct tgtgacggaa
    22681 ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga cgcccctgaa
    22741 aattcatttt attcaccaga tcaggaagac gtggcattgc catttcctga gctcctggct
    22801 aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg
    22861 ccagaggtag taactcaact actcgaactg gagttagcag gatggatcgc agctgtaccc
    22921 ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt atttgtttga
    22981 aacctatatt cacacagaag ctgagttgcg tgtggatcaa gacaaacttg aacaggatct
    23041 taccgacgca ggatttgatc gagaagatat ctacaatgcc ctgctttggc tggaaaagct
    23101 tgcggattat caggaaggac tggcagaacc gatgcaactg gcctctgatc cactctccat
    23161 gcgtatttat acccctgaag agtgtgaaag gctggatgcc agctgtcgtg gttttctgct
    23221 tttccttgag caaattcagg tgcttaacct tgaaacccgt gaaatggtga tagagcgagt
    23281 gctcgcgctg gataccgcag agttcgacct ggaagacctg aaatgggtaa tcctgatggt
    23341 gttgttcaat attccgggct gcgaaaatgc gtaccagcaa atggaagaat tactctttga
    23401 agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt gttatggcga
    23461 aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg gctgaactgg
    23521 ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg gcgtgtgact
    23581 acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg gaggggcagg
    23641 tttgccctgc atgtggcgca aatctggtat tacgccaggg acgctttggt atgtttattg
    23701 gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg gacgaaacag
    23761 caattacctg tccccaatgt cggacgggcc atctggtcca gcgccgttcc cgttatggca
    23821 aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac ttcaaaccca
    23881 tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa accgcgcagg
    23941 gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg gaataataac
    24001 gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct caatgaaaaa
    24061 cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc tgatagcgaa
    24121 acagcagtga tgcgactgtt ggagttaaaa cagcgtccgg ttgataaggg gctgatttta
    24181 atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgct gactgatgcg
    24241 cagcgtgaaa ccattttttc ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct
    24301 gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg agtcaccgac
    24361 catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc taccagtgcc
    24421 aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg
    24481 gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga aatccgcgat
    24541 gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg ctgtttttgg
    24601 taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc agcaactgaa
    24661 tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca acacactgaa
    24721 cgctttcttt agtgctggtg gtaaaggtgc gaatgtgacg gtacctttta aagaagaggc
    24781 ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgctg ttaataccct
    24841 caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtatag gtttgttaag
    24901 cgatctggaa cgtctgtctt ttatccgtcc tggtttacgt attctgctca tcggcgctgg
    24961 tggagcttct cgcggcgtac tactgccact tctttccctg gactgtgcgg tgacaatcac
    25021 taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca ctggcagtat
    25081 tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta ttaatgcaac
    25141 atccagtggc atcagtggtg atattccggc gatcccgtca tcgcttattc atccaggcat
    25201 ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat ggtgtgagca
    25261 gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg cggctcatgc
    25321 cttccttctt tggcatggcg ttctgcctga tgtagaaccg gtcatcaaac tattgcaaca
    25381 ggaattgtca gcgtgaatca ggccatccag tttccggata gggaagagtg ggacgagaat
    25441 aaaagatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg tgcgatctct
    25501 ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt agcgagtttt
    25561 cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga acaaagtgaa
    25621 gatgatcaag gttgggtctg gttaccctga tccagatatt cgtccttcca tttcacgtaa
    25681 ttcttcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg gatctgtttg
    25741 acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg gaccaggcta
    25801 cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat tgagcccatc
    25861 ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg accaacagtg
    25921 acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt atgagtgaca
    25981 tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata atgtacatct
    26041 ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac gtcaccaatc
    26101 acgacgctgc tatcgtcgat cattacgcgc tggccgattt gtggaaaaag atcgcggtat
    26161 gggcgtaaaa catcagacat actcacctca acaataaatg atttactaat gactttgggt
    26221 gcattattgg ccttgtgcaa gtcttttagc atgcaaaaaa gcaccgtttt gtgtgcgaat
    26281 gcagcaaaaa gggtgaaaaa gcaacaaaca gaaaaaaaga tcaaaaaagt acttgtgcaa
    26341 aaaataggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca cttcacaaga
    26401 tggtcgtaac aacaaagaga aaaaatcctg aaattc
//
LOCUS       DAZGHF010000065        26389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGHF010000065 DAZGHF010000000
VERSION     DAZGHF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.065"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(51..2123)
                     /locus_tag="ACHK3A_002774"
     CDS             complement(51..2123)
                     /locus_tag="ACHK3A_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO8740831.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     VHNPWGLAIVIAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPHIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMEFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDYAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDIYTQ"
     gene            complement(2120..3661)
                     /locus_tag="ACHK3A_002775"
     CDS             complement(2120..3661)
                     /locus_tag="ACHK3A_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase"
                     /protein_id="HFO8740832.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHIMAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRKVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWMSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(3671..4447)
                     /locus_tag="ACHK3A_002776"
     CDS             complement(3671..4447)
                     /locus_tag="ACHK3A_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO8740833.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQVANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(4457..5299)
                     /locus_tag="ACHK3A_002777"
     CDS             complement(4457..5299)
                     /locus_tag="ACHK3A_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO8740834.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNINVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(5309..6100)
                     /locus_tag="ACHK3A_002778"
     CDS             complement(5309..6100)
                     /locus_tag="ACHK3A_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO8740835.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            6313..6960
                     /locus_tag="ACHK3A_002779"
     CDS             6313..6960
                     /locus_tag="ACHK3A_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO8740836.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKNNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(6944..7582)
                     /gene="ytfB"
                     /locus_tag="ACHK3A_002780"
     CDS             complement(6944..7582)
                     /gene="ytfB"
                     /locus_tag="ACHK3A_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO8740837.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            7800..8420
                     /gene="fklB"
                     /locus_tag="ACHK3A_002781"
     CDS             7800..8420
                     /gene="fklB"
                     /locus_tag="ACHK3A_002781"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO8740838.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8729..10141
                     /gene="cycA"
                     /locus_tag="ACHK3A_002782"
                     /gene_synonym="dagA"
     CDS             8729..10141
                     /gene="cycA"
                     /locus_tag="ACHK3A_002782"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO8740839.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(10186..10848)
                     /gene="ytfE"
                     /locus_tag="ACHK3A_002783"
     CDS             complement(10186..10848)
                     /gene="ytfE"
                     /locus_tag="ACHK3A_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO8740840.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(10956..11921)
                     /gene="ytfF"
                     /locus_tag="ACHK3A_002784"
     CDS             complement(10956..11921)
                     /gene="ytfF"
                     /locus_tag="ACHK3A_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8740841.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPGFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            complement(12029..12889)
                     /gene="qorB"
                     /locus_tag="ACHK3A_002785"
     CDS             complement(12029..12889)
                     /gene="qorB"
                     /locus_tag="ACHK3A_002785"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO8740842.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            12978..13358
                     /gene="ytfH"
                     /locus_tag="ACHK3A_002786"
     CDS             12978..13358
                     /gene="ytfH"
                     /locus_tag="ACHK3A_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO8740843.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(13487..15430)
                     /gene="cpdB"
                     /locus_tag="ACHK3A_002787"
     CDS             complement(13487..15430)
                     /gene="cpdB"
                     /locus_tag="ACHK3A_002787"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO8740844.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAVFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            15620..16360
                     /gene="cysQ"
                     /locus_tag="ACHK3A_002788"
     CDS             15620..16360
                     /gene="cysQ"
                     /locus_tag="ACHK3A_002788"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO8740845.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAVAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(16350..16907)
                     /gene="ytfJ"
                     /locus_tag="ACHK3A_002789"
     CDS             complement(16350..16907)
                     /gene="ytfJ"
                     /locus_tag="ACHK3A_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO8740846.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            17232..17438
                     /gene="ytfK"
                     /locus_tag="ACHK3A_002790"
     CDS             17232..17438
                     /gene="ytfK"
                     /locus_tag="ACHK3A_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO8740847.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(17500..18843)
                     /gene="paeA"
                     /locus_tag="ACHK3A_002791"
     CDS             complement(17500..18843)
                     /gene="paeA"
                     /locus_tag="ACHK3A_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO8740848.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(19166..19804)
                     /gene="msrA"
                     /locus_tag="ACHK3A_002792"
     CDS             complement(19166..19804)
                     /gene="msrA"
                     /locus_tag="ACHK3A_002792"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO8740849.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            20010..21743
                     /gene="tamA"
                     /locus_tag="ACHK3A_002793"
     CDS             20010..21743
                     /gene="tamA"
                     /locus_tag="ACHK3A_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO8740850.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            21740..25519
                     /gene="tamB"
                     /locus_tag="ACHK3A_002794"
     CDS             21740..25519
                     /gene="tamB"
                     /locus_tag="ACHK3A_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO8740851.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSMPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            25522..25863
                     /gene="ytfP"
                     /locus_tag="ACHK3A_002795"
     CDS             25522..25863
                     /gene="ytfP"
                     /locus_tag="ACHK3A_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO8740852.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(25982..>26389)
                     /locus_tag="ACHK3A_002796"
     CDS             complement(25982..>26389)
                     /locus_tag="ACHK3A_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8740853.1"
                     /translation="RWGEVANIKGEHIIGGKVIFMETKNGSRRVIPIAKALESLIKVK
                     ATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHTFATHFMINGGNIITLQRILGHS
                     TIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
BASE COUNT         6358 a         6711 c         6940 g         6380 t
ORIGIN      
        1 atttccatgt aataggcaat aaaaatcagg tacagaggaa atattgaaaa ttattgtgtg
       61 taaatgtctc cctttaaaaa atctggccag tttttttcaa agtaaatacc gttaatacac
      121 ggttgcatac gtaaagactt tacttcggta ataaaagaac cttgcgaata ttgaattgtt
      181 aatttgtgat ttttgtcttc agcgagttca gagcgaaaac gccattcttc ttttgcgccg
      241 cgacgagttt gtggtaggca tttgccttgt ggatcaataa ccatacgcgc cccacggcta
      301 ccaccaccag catcaatata atgcgttaat tgtgttaata ccgcggcaga agtcagcgcc
      361 atatgccgcc acataaacag ctcggcaact tcacccacat gtttgattgc caatccatgc
      421 cgttggacaa attcgttcaa tagcagggca tcacgagtgg cacgtcggac tttatcggca
      481 tggcaaataa atcccgcata gtcagacatt cgtgcctgaa ttttttctct cacaaccgac
      541 aacggcatcc cggtcccgtt atcgtgcgcc tgagtaatga tctctcttat agaagccagt
      601 gccggagcta ccagctgtgc tatatctcca tcaatattac gtttttgcgt gcaaccaata
      661 aaacgtgcca gacgaacagc aaaaacctgc ccggcattca atgccgcacc accagggcga
      721 gtgacgccgt gtgtgccagc aacttccccc acggcgaaac agccgggcag ggaggtttgt
      781 ccccagatat ccacttcaat gccgccattc atatgctgat tattcatggc aaactgcaaa
      841 ggctgtgtgg tgagatcgta accgtgcatc ttatacagcg agatagacag cggattcatt
      901 cgttgcagcc gttcgatggg cgatggtgcc agagcgtcgt tattttccag atacgcgcga
      961 acgtcgtcgt ccagtcgatc taatgagaag ggcaggtcac ccggcaccgc ttcaggattg
     1021 cgattgaaat ccataaatac ctgacgccct gattgctgct cttgcgctac agccatatct
     1081 aacaagctgg agccaaactc cattacccga gtggcgtgga acggccactg atagccttta
     1141 cgaaaaatgt ttgaagccag ctcctgggtg gtgcgatagt aatccgcgag gaagttatac
     1201 tcgttgcccc cggcatccac ggaatagata tgtgggatca cctgcacata ggtgccggac
     1261 aaattccacg gaaacgtgct gcgcggtgtg cctataccaa actggctttc agtcaaattg
     1321 gttagcgtca gaccttcttc cagcgccagc cccagcgagc cgaaacattt gtgtgggtac
     1381 acactgtcgc gataaagctc gccaggccct cctgttgcca gtaccacatt gggcgcaatc
     1441 acaattgcca gcccccatgg gttatggacg cgatgaccgg tcgcgaggat agcccccgcc
     1501 acgcggtctt cgccgttttc gtcacgctga tgcagcagtt taatcactgt tgcactggtc
     1561 aacattggga tagcaagacg ctgtacttct tccagcagca ctttcaccat taaccttgag
     1621 gtacgtggcc cacacgaggt tgcacgcccg gcttcgtcat ggtcggtttg ataacgaaga
     1681 atcgcaccat agcgatcttc cggtaattcc agaccaagat attgcaaccc gccaagagtg
     1741 tgcagagaac ctaccgcttc gacataagca gtgtcgtgat ccatcgcccc accactcgcc
     1801 agtgcctctg ccagtttggt gaagttgtcg ccgttgcccg cggtagcggc ggtaaacagc
     1861 gtctgtttat cggaaccgga gcaggccgat gttcccatgt ataacccggc ggtggcgatt
     1921 aacacatttt gctggcggcg tttaagttcc acagctgcac gcagcccggc tgcaccactg
     1981 ccgactacca gggcatctgt ttgccaaagt gggtaatcaa caccgtctac cgtgacaacc
     2041 catgcgggtt ctgcctgtgc aaaacccttt gggaaagtaa cgtgcgtcag cgcatcaagg
     2101 atggtgtcat caagctgact catgcatcct ccatcggcgt aaacaggcta ctttccatca
     2161 ggcgtgggtg cttgataatt ggacggaaag ccatatacgg cagaatgtcg cgctcaatct
     2221 ccacacccgg cgcgatttca attaattcaa cgccttctgc cgttaactgg aatactgcgc
     2281 gttcagtgat atacagcact tcctgaccac tttgctgtgc gtaggcggcg ctaaaagtga
     2341 ttttttccac ttgggtaacc agtttaggga tttgacctga ctgggcgata tgcaggccat
     2401 caggcgttac atcaatcttg ctgcctttgg cgtcaaacgt accgcagaac accactttac
     2461 gggcgttttg cgcaatttcg aggaagccgc cggggccaat gagattgcca ttgagatggg
     2521 atacgttgac gttaccgtac cgatccatct ctcccatgcc gaggaaggtg atatcgatac
     2581 cgccgccact gtagaattcg aattgatcga gtgatggaat aatggcgtcg gcgttacggg
     2641 cgcagccgaa cagagcatca tccagcatca tgccgttatg tacgccttgc tcgacgctca
     2701 tccataaact ttgataaggc acgccttcgc gcagcgcaac cccaggaata ccgcccggaa
     2761 taccaaagcc aaagttggtt gaagcgcctg ccaccagttc ccgtgctgcc cgacgggcaa
     2821 tcaggcgacg caccggatga ctaaccagtt caatagcgtc gttagaactt agacggcgat
     2881 gttgaccgct gatcgtcagg tcgtaaccgc caagataggt ttgcggttgt tcgcggtgtt
     2941 caacgatacc atcgaccaga atccccggta gcttgacctt gcgaggttca atggcgccag
     3001 cttccagtac atcgcgcacc tgtacaaaca ctttgccgcc actgttgtgt gctgccagcg
     3061 ccatcgaata gctatccata tcaatcgctt cttcttcgag gctaacgttg ccacgcggat
     3121 cggcataggt gccgcgcaaa atcgcgtagt ccactttgaa agggcgatag cggagtttgg
     3181 tttcaccgtc gatttcaatc agttctacca ggtcgtcggt cgtgcattca ttcgacttac
     3241 cgccaccatt gcgtggatca acaaacgatc ccagcccaac gtgggtaaaa agccccggac
     3301 gtccggcacc gatctcccgc agcagtgcct gaattacgcc accagggaaa cagtaggctt
     3361 caatagcatt gcttttcacc agttcctgca tcttgggcga ccaggtaaaa tgtccggcaa
     3421 taatgcgttt gagcatttcg gcgtgagcaa aacggttagt gcctttgctg tcgcgatcgc
     3481 caatacccag cgagtggatt aatgtcagat cgcgcgggtg tccggtttgc aggaaacgcg
     3541 cttcaatggc ggccataata tggtcagctt ccaccatacc gccgccgtta ccgctaatgg
     3601 caatggttgc gccgtcctga atttgcgccg ccagtgtctc ggcggttgat aacttacgca
     3661 tacaatttcc ttattgattg tggaagctca cgccggcttt ttcggcaaat gcgtgcgtcg
     3721 cctgctggca atcctgagtg gcggacatta atccagccag taactggtgg gagtaatcgg
     3781 cgtgtttatt ttccagcgcc agaatcagtt gcttaatatg tgccagtgcc accggaccac
     3841 atttttccag ctgcgctaac tggttcgcca tccagctttc cacctgttct ttttctacca
     3901 cttcatcaat taaattcgcc acctgggctt cctgcgccgt caggcgttta cccagcatca
     3961 gcatctggcg accgaccacc gggccaacct gattaagcac gcgttcaatg cccatccagc
     4021 caggcaccat gccgagcatc acttccgggt tagagaattt cgcgccgggg cgagcgatac
     4081 gaatatcgca gcacaaggca agttcaatac cgccaccgat ggtatgaccg ttgagattcg
     4141 caacggttaa ttggggtaag ttgcgcaggc gattaaaaac ctcgttaccg cgtttaatcc
     4201 attttctgcc catatctaat ggcgaatagg ccgaccacga tttaatatcg ccgcccgcac
     4261 aaaagaattt atcgccggtt gccgttaatt cgactaatcg taatgtggtg tcgttttcaa
     4321 tttcatttaa ataatggtcg agagattcga tcatttcttc attaatagca tgacatttat
     4381 cttcacggtt caatgtcagg cggcaagaag ccgccgctct gctgaacaga acgggttggt
     4441 cactcatgat tattccttag caataatgtg cgatgcgatc gacgctaata tccagcgtgc
     4501 tgttaatatc tttctgccac cagtacggtg agaatatttc cagttcaata gcgccattaa
     4561 atccggcgtt ttcaaccagc cgacgaattg acggaatatt gatgacacca tctcccggca
     4621 ttccacggtc attgaccaga tcggtggtcg gcactaacca gtcggaaaca tgaaacgcga
     4681 ggatgcgttt tccggcacgc aggatctgac tggcgagatc cgggtcccac cagacatggt
     4741 agacatctac cgccacgccg aggccaaatt cgccatcggg atcgagttca tcacaccagt
     4801 ccagcgcctg acgtaacgtg cacagacaag aacgatcggc agcagtcatc gggtgcagtg
     4861 gctccagtgc aattggcacg ccgacctctt tcgaatgtgg cagcaactgg cgaatccctt
     4921 gtttcacctg ttcccgtgct tcgtacagat cttttgttcc cattggcaga ccgccaacca
     4981 cctgcatgta acaggcggca tttagcacag cggcatcgtc cagcgcccgg cggttatcat
     5041 caatcgccag cttgcgctcc gccagcgtcg gtgcggtgta gtaggtgcta cggcacaaac
     5101 cggaaacgtt tatgttactg gcagcaagct ggcgggcgat ttgctggaga tcttcaccct
     5161 gcaactccct gcgccagggg gcaatggcgc caataccgcg tgcggcgcag gcatcaataa
     5221 tggcgggtag cggcgtttta tagcccagtg tcgcggtatt gatactgaat aaatccgggc
     5281 gttgtaacag atctctcatg attcagcctc agaagcgcgg catacttaag ccgccgtcga
     5341 tggcgaccgc ctgaccacag gtgtaaatca gtttaccttc cgccatcgcg cggacagtgg
     5401 aagcaatatc agccggataa ccccaacgtc cccacggcac cagacctttg gcaatcagtt
     5461 cgtcgtaata ggccgttgcc ggaatagtca tatcggtttt gatgagacca ggacgcactt
     5521 catatacgcc aatttgttca ttacaaaggc gagcagcaaa tagacgagca gcggcagaga
     5581 cggcagtttt ggcaatggta tattcgccgc gattcatcgc cagcatgatg gcgttgatgg
     5641 aactgacgaa aataatcgag cggtgcggca gttctgcttc cggtttcggc tgtgccagta
     5701 agcgcttact gaatgcctga gcgaggaaga acggtgcgcg ggtgttaatg gcaatatttt
     5761 ggtcgaagct gtctggttcg aggtcgagaa gatcaccgcg ttttttcact gaaatgcctg
     5821 cgttgttgag caggcagtcc agacgtcccc actggttttg cgctgcgtcg agcatctctt
     5881 catgcaggga gagatcgccc acatcagcag ggaagcaaat cacttccacg ccttccgcca
     5941 gacattcttg ttgtgtggca tgtaattttt ctacgctatc cgcatccgga cgatcattaa
     6001 tcagcagatt aaatccgcca cgcgcaagct ccagcgcaca gccctttcca ataccacgag
     6061 cggctccggt aatcacggca acaggacgag tggatttcat acaatagctc tctttttttg
     6121 aattttgttt ccttagtgat gattagcttg cgactggagg gtaaaaagat caaccatcta
     6181 gttggttgat atgcgatggc gatcacagga catttgataa cctgatgaaa gtacaagttt
     6241 ttgccctttt ttctgaagct gaaatcaaat caggttatga tagtcaccac gaagaggtcg
     6301 tggggaagcg ttatgtatct tgataccagc aacgtacaga gcctgaaaga aaagctgctg
     6361 ttatgcgcgg ttaatgaatt tgccgaatat ggttacgaag gggccagagt cgataatatc
     6421 gtcaaggccg cggggtgcag taaacagacg gtttatcacc actttggtaa taaagaaaat
     6481 ttatttatcg aagtactgga atatacctgg aacgatattc gacaaaaaga aaaggccctt
     6541 gatttctcag atcttccgcc acagaaagcc attgagaaaa taatcgattt tacatgggat
     6601 tattatattt ctaatccctg gtttttaaaa attgtgcata gtgaaaatca aagtaagggt
     6661 gtgcattatg caaagtcaca gcgtctttta gaaattaacc atgcgcactt gcagttaatg
     6721 gaatccttat tggatgaagg gaagaaaaac aatattttta agccagatat cgacccattg
     6781 caggtgaata ttaatattgc tgcgcttggg ggatattatt tgatcaacca gcatacgctt
     6841 ggtctggttt atcacatcag tatggtttca ccccaggcac tggaagccag acgtaaggtc
     6901 atcaaagaga caatccttag ctggcttttg gttgaccctt catctaccgc gcgcgaataa
     6961 aactgccatc cggctggcgg gtaaacagga cctgttgatt attccccgta tcaatggtta
     7021 agcccgtcac cacgccgctg gcgttctggc ggattttcac catctgcccg ttttgcaaat
     7081 tactcagtgg cttatccgcg ccttctacct gcgccatggc atagacatcg gtagcgggca
     7141 gtccgtggtc gcggaacagc tgcgccatcg ttttaccggg ttcgacacga taggagcgcc
     7201 attgattatc aattccgcta tctggctgga atggttgcgt atgggtggtt tgcggctgct
     7261 cttctggctg gccttcctgt attggttctg gagcgaccgg agccacctga tcaggatcat
     7321 tttgtggcgt gactaattgc gcccgcagct gttcttctgt cggtggttgt gattgcgact
     7381 gaatatctaa ctgcgcttca cgcgtcacca ccggcgcgtt gggcgtatca tcagatggaa
     7441 gcagaaatcc gactaccaat acaatggcgg caataatgat cccccgacga tgcatcggcg
     7501 gcagcgggtc catgaagcga aaattgtccg gcgcgtgcca gactttctcc agggttggtt
     7561 ttagttcaaa gcgcccgggc atggtttcct cctgctccgc gtcttgttcc tcaatcatag
     7621 cctatgaata agctaacgct atgatgtccg tggtaaaccc gcctttatta tattcatacg
     7681 ggatattgct attgtttctt tttccccggg atttgtcatc attcccgcga caaagtttta
     7741 cccaaagaag tgtggctgat atgctgcccg ctactttata ccctaagaaa ggaaatacga
     7801 tgaccacccc aacttttgac accatcgaag cgcaagcaag ctacggcatt ggtttgcagg
     7861 tagggcaaca actgagtgaa tctggcctgg aagggctgct gccagaagca ctggttgcag
     7921 gcattgccga tgcgctggaa ggcaaacatc cggctgttcc ggttgatgtg gtgcaccgcg
     7981 cgctgcgtga aatccacgaa cgcgccgatg ccgttcgtcg tcagcgtttc caggcgatgg
     8041 ctgctgaagg tgtgaaatac ctggaagaaa acgccaaaaa agaaggtgta aatagcaccg
     8101 aatctggcct gcaattccgc gtgatcaacc agggcgaagg cgcaattccg gcacgtaccg
     8161 accgcgttcg tgttcattac accggaaaac tgatcgacgg caccgtgttt gacagctccg
     8221 ttgctcgcgg cgagccagct gaattcccgg ttaacggcgt gatccctggc tggattgaag
     8281 cactgactct gatgccggta ggttctaaat gggaactgac tatcccgcag gaactggcat
     8341 atggcgagcg tggcgcaggc gcgtccatcc ctccgttcag caccctggtg tttgaagtcg
     8401 aactgctgga aatcctctaa gcagcgcatt ctgttcccct cgaaccagag gggagcagac
     8461 attcagcaat aaacccttca gtttgccaaa cggcgctatt ttgtgttgca aagaccccgt
     8521 aagcgtgtat ttttgtgagc tgtttcgcgt tatcaccgtg atatgacact cactttaaac
     8581 ataaaattaa cattagatct aaatcttagt attcataccg cgtattgtta cctaatatcg
     8641 atgagtcccg atacagattc gtcgtatcat agactgacta aaggccgtag agcctgaaca
     8701 acacagacag gtacaggaag aaaaaaacat ggtagatcag gtaaaagtcg ttgccgatga
     8761 tcaggctccg gctgaacagt cgctacggcg caatctcaca aaccgacata ttcagcttat
     8821 tgccattggc ggtgccattg gtacagggtt gtttatgggg tccggcaaaa cgattagcct
     8881 tgccgggccg tcgatcattt tcgtttatat gatcatcggt tttatgctct ttttcgtgat
     8941 gcgggcaatg ggggaattgc tgctttcgaa tctggaatac aaatctttta gtgacttcgc
     9001 ttccgattta ctcgggccgt gggcaggata tttcaccggc tggacctact ggttctgctg
     9061 ggttgtaacc ggtatggcag acgtggtggc gatcacggct tatgctcagt tctggttccc
     9121 cgatctctcc gactgggtcg cctcgctggc ggtgatagtg ctgctgctga cgcttaacct
     9181 cgccaccgtg aaaatgttcg gtgagatgga gttctggttt gcgatgatca aaatcgtcgc
     9241 cattgtgtcg ctgatagtcg tcggcctggt catggtggcg atgcactttc agtcaccgac
     9301 cggtgtggaa gcgtcattcg cgcatttgtg gaatgatggc ggctggttcc cgaaaggttt
     9361 aagtggcttc tttgccggat tccagatagc ggttttcgct ttcgtgggga ttgagctggt
     9421 aggtacaaca gctgcggaaa ccaaagatcc agagaaatca ctgccacgcg cgattaactc
     9481 cattccgatc cgtatcatta tgttctacgt cttcgcgctg attgtgatta tgtccgtgac
     9541 gccgtggagt tcggtagtcc cggagaaaag cccgttcgtt gaactgtttg tgttggtagg
     9601 tttgcctgcg gctgccagcg tgatcaactt tgtggtgctg acctctgcgg cgtcttccgc
     9661 taacagcggt gtcttctcta ccagccgtat gctgtttggt ctggcccagg aaggtgtggc
     9721 accgaaagcg ttcgctaaac tctctaagcg cgcagtaccc gcgaaagggc tgaccttctc
     9781 ttgtatctgt ctgctcggcg gcgtggtgat gttgtatgtg aatcccagcg tgattggcgc
     9841 gttcacgatg attacaaccg tttccgcgat tctgtttatg tttgtctgga cgattatcct
     9901 ttgctcgtac ctggtgtatc gcaaacagcg tcctcatctg catgagaagt cgatctacaa
     9961 gatgccactc ggcaagctga tgtgctgggt atgtatggcg ttctttgtgt ttgttctggt
    10021 gttgttgaca ctggaagatg acacccgcca ggcgctgctg gttaccccgc tgtggtttat
    10081 cgcgctgggg ctgggctggc tgtttattgg taagaagcgg gctgctgaac tgcggaaata
    10141 atcgcattat catgctggat ggcgcaatgc catccagctt ttagatcact cacccgccag
    10201 cgcgcgcggg aatagtacat tgttttccag actgatgtgc tccatcaggt catcaatcag
    10261 ttcattaatg ccgttataca tcgctttcca ggtggtgcag gcttccggcg gcggtgtgac
    10321 gttattggtg gtgtgtttga tcacttccag cagttcgccc gcttcatcgt gctcgctttc
    10381 cattacgctg attggcccca ttgcctggct gcccatgcct tgtttaatca tcgggaagag
    10441 aatttgctct tctttcatca tgtggctgga aagctcttca tgcagcatag tcaggtattt
    10501 tgtcagccct tttggcacac ttggtttgtc ggcgtgaacg cgctcgactt tagtcgcttg
    10561 caggatcagc tccggcagtt gctcgcggtg gcgatcgtgg tagcgcacga tgatatggtc
    10621 gatgatttct gccagcgggg cgctacgcca gtctttctca atcggttgtt cagcaagctt
    10681 tgccagttcg gcttcaatga cgtcaacatc cagttcttta cgtgccgccg cgcgcgccag
    10741 cgtctgctta ccgccacagc agtaatccat atcatattta cgaaacagag ccgaagcgcg
    10801 aggaatagag agcgccagtt cacctaaagg ttggtcgcga taagccatag ctgatacctc
    10861 attcttaata agataagatg tattttaaat gcatctttat ggcaaatgct ataaccctta
    10921 cgtagcagag gcttatttaa ctcactgcca gcgtgtcaac tttctgagac agattcagtt
    10981 aaaggttttt ctggttttgc tctgactgca atgaccacgc caaccactaa cagcgcgata
    11041 ccgcttagcg tcattagcgg cggcatttgc tggcgtaaca aaaaggtgta cagcaagcct
    11101 gccagcgttt cgaaaacaat cagcggcccg agaatcactg tcggtaatcg ctggctggcg
    11161 acgttccagc agagtgcgcc aacccatgag caaagcacgg ctatcgcaac catcagacta
    11221 ataaacacca gcggacgggg gccaaagggt agggagaagc ccggcgtttg cgtattcagc
    11281 cagtaacagg cgacgagata gccgatgaga gaaaccggca gcgtgaccag cgcctgcgcc
    11341 gtcgcccaca tcatcggatg tttgtcggga ttctcccgca gccagcgggc gttgcgcagg
    11401 gcataccatg cccagcagac cacggaaact aacgccagca cgatgccaga ggtataacgt
    11461 gcccagtcaa aatcggggag tccgtggttt aactcagcaa tattcacaca cgccaggccg
    11521 atgccaatac aaaccagtgc cggggcgagt tttccccacg cgagtttgcc gtcgcgctgg
    11581 ctataaagca gattggcaaa aacgggaatc accaccggca gggtgccaat aatcatcgtg
    11641 gaaacaggcg cgccagtacg ttgaatggca ctggcaaggc agaagtaata gatgaggttg
    11701 cccatcatgg tgagcatcaa ggcggtaagc cagtcgcgac gcgccaactg acgcagacgc
    11761 acgcgtccca gccaggcaat gggcagcgca attaacccta acgccagata acgccccatc
    11821 gactgcaaca tcgccgggta ttccggcacg atcaacggcc cgacaaaaat aagcccccac
    11881 atcaaccctg ctaacagggc gtacagcacg ccgctaatca ttactggcat ccattggtct
    11941 gtcagaagaa agttcaggaa gcagacatga ttgctcccgg catcccgtca ttatcagaga
    12001 ggatgcggga ggatgccact ttaattaact agttattaac attaaaaaga tggcttacgc
    12061 tttcggctaa cgttgtcgtt gggcggccaa tcaatttgct aagcgttttg ctgtcatcaa
    12121 acagaccgcc tttcgatgcg ccaacgtcag aatccgccag catatccgcc agtccatcag
    12181 gcagtccgac gcttttcagt gccgcagcga aatcagcttc gctcaggttt tgataggtaa
    12241 cctgtttgcc gctctgtttg gtgagttctg ccgctaactg tgtcagcgtc caggcactat
    12301 cgcccgccag ttcgtaaacc ttgccttcgt gaccggcttc gctaatcacg cgtgccgcag
    12361 ctaccgcata atcagcccgc gttgctgagg caattttgcc atcgcctgcg gcaccgataa
    12421 atacgccgtg ctccagtgct gccggggcgc tggcgaggta gttttcggtg taccagccgt
    12481 tgcgcagcag ggtgtaaacg atgccagaat cagccagcat tttctccgtc tcgatgtgct
    12541 catcggcgag gccgagcggg gaggaatcgg catgtagcag gctggtataa gcgataaatt
    12601 tcacgccggc cgcctttgcg gcattaataa cattacgatg ctgcggggca cgttgaccca
    12661 cttcgctgga agagatcagc agtagttttt ccactccctg aagtgcagat gtcagtgcgg
    12721 cttcatcgcc gtaatcagcc tgacgcacgg taatgccttg tgctgtcagt gcctgtgctt
    12781 ttgccggatt acgaacgata gccactattt ggctggcagg aaccgttttc atcaaggatt
    12841 caataacata gtgaccaagt tggccagtgg caccagtaat agcgatcatg ggaagtctcc
    12901 atcgttttgt cttgtgttta aatacgctaa cacttaaact tacttttagt aagtacgtac
    12961 aaaaaggtaa gtatgagatg agtcaggtta gcctgtcgca acaactgaaa gagggaaatc
    13021 tcttcgctga acagtgtccg tcgcgcgagg ttttgaaaca cgtcaccagc cgttgggggg
    13081 tgttgattct ggtggcgcta cgcgaaggta ctcatcgctt tagcgacctg cggcgcaaaa
    13141 tgggcggggt gagtgaaaag atgcttgcgc agtcgttaca ggcattagaa caggatggtt
    13201 ttcttaaccg tatcgcgtat ccggtggtgc cgccgcatgt ggaatatagc ctcacgccgc
    13261 tgggcgagca ggtgagcgaa aaggttgctg cactggcgga ctggattgag ttgaatttgc
    13321 ccgaggtgtt agcggtgcgg gatgaacgtg cggcataact gccggatgcg ctgcgcttat
    13381 ccggactacg gttcatggct tgtagggcgg ataagatgcg tcatcatcgc atccgccaat
    13441 aaatgccgga tgcggttgat gatttgtagg ccgaaaaaga agtgttttac ttactcagat
    13501 ccacctgata aatcgcaaac ccgatatcat cggtcgcgac tttattcatc ggatactgcc
    13561 ctttctcttt aataaacact gcggctttat ctgacggaga ggtttcgaaa cggatatcca
    13621 gtttcttatc gccagctatt ggtgctaaac gccagttgtt atctgccgcc gggtgaattt
    13681 cccccgcacg tttcgactca tcagcaatcc acgctgccag caccgagcgg ttctcatccg
    13741 gtgaagcaaa agcgatatgg ctgtcgcccg taccggcaaa tttgccgccg taagcgcgat
    13801 agttattggt ggcaacgagg aacatggcgt tcggatcaat cggcttgccg ttaaaggtca
    13861 ggttcttaat cctttccgca ttggcattaa tcatctggca ctcgccgtca taacgggcgg
    13921 gctgggtaac atcaatctga taattcacac catcaataac atcaaagtta taggtgcgga
    13981 aaccatccca gttgatgagt gactgtggtt tcgtgctgtt aggatcaatc tggttaaact
    14041 gtccggcgga gcactccagc cactctttca cctctttacc gctggctttc accacaatca
    14101 gcgtattggg gtagagataa agatcggcgg cattacggaa ggtcaactgg cctttttcca
    14161 cctccacata gcttgccggg tcatttttgc gaccaccaac tttaaacggt gcggcagctg
    14221 aaagcactgg cagttttgcc agatccggat cgccctgaat gtaatgctcg acatacgctt
    14281 tttgcgcgtt gttcaccacc tgcacggtcg gatcgtcctg cacaagcgcc agatagctat
    14341 acatattgtc ggcagattta ccgattggct tgctgacgaa ctggcgtgtg gcatcgtgat
    14401 cggctttgag tgtttctacc agcttgctgt cttccgccgc gagggatttt ttattcgcga
    14461 tgtcgtaaat cggccgtgct tccgctttcg cctgcgtcac ctgccattta ccgctgtcat
    14521 tactgagttg taagtcgacc acaccaagat gatcgcccca catgcctggc attaccgccg
    14581 gaacaccatt cagcgtgcct ttcgcgatat cagccccttc gatatcagca aaatctttac
    14641 ctgggaaaac ggcgtgagca tggccaaaca taatggcgtt aacgcccgga atttcactga
    14701 ggtaataaac tgagttttcc gccatcactt tatacggatc ggcagacagc ccggaatgcg
    14761 ccagaacgac aacgacatcg gcacctttct cgcgcatttc aggcacgtat ttgcgcacgg
    14821 tttcggtaat atcattcacc gtcactttcc cggataaatt agctttatcc cagcccatga
    14881 tttgtggcgg gacgacgcca atataaccaa tcttcagcgt ctgttttttt ccgtctttat
    14941 cgaccacttc ggtgtcttta attaaatacg gtgtaaacat tggctgtttg gttctggcgt
    15001 caatgacgtt ggcatttaca taagggaatt tcgctcccgc cagcgcattt ttcagataat
    15061 ccagaccgta gttaaactcg tggttgccaa gcgtgccgac ggtatagtcc agcgtattta
    15121 atgccttata gaccgggtga atatcacctg cttttaaacc tttcgccgac atgtaatctg
    15181 caagcggact cccctgaatc aaatcgccgt tatcgaccag tacgctgttt ttcacttcat
    15241 tgcgggcatc gttaatcagg cttgcggtac gtaccagtcc gaatttttcc gtggcggtgt
    15301 ctttgtaata atcgaaatcc atcatgttgc tatgcagatc agtggtttcc atgatacgca
    15361 gatcgaccgt cgctgcattc acactggcgg caatcagcgt ggccaggagc gttgcgctaa
    15421 acttaatcat cagggacatc cttttatcat cgggaatacg aaagaaaagg gagaataaac
    15481 gtcttactta tagaacagtg aagaatgcca caattttacg ctttgaaaat gatgacacta
    15541 tcacagttgg cgcattcatt aacgataggg tataagtaaa acaataagtt aacaccgctc
    15601 acagagacga ggtggagaaa tgttagatca agtatgccag cttgcacgga atgcaggcga
    15661 tgccattatg caggtctacg acgggacgaa accgatggac gtcgtcagca aagcggacaa
    15721 ttctccggta acggcagcgg atattgccgc acacaccgtt atcatggacg gtttacgtac
    15781 gctgacaccg gatattccgg tgctttctga agaagatcct cccggttggg aagtccgcca
    15841 gcactggcag cgttactggc tggtagaccc actggatggt actaaagagt ttattaaacg
    15901 taatggcgaa ttcaccgtta acattgcgct catcgaccac ggcaaaccga ttttaggtgt
    15961 ggtgtatgcg ccggtaatga acgtaatgta cagcgcggca gaaggcaaag cgtggaaaga
    16021 agagtgcggc gtgcgcaagc agattcaggt ccgtgatgcg cgcccgccac tggtcgttat
    16081 tagccgatcc catgcagatg cagagctgaa agagtatctg caacagcttg gcgaacatca
    16141 gaccacgtcc atcggctctt cgctgaaatt ctgcctggtg gcggaagggc aggcgcagct
    16201 gtacccgcgc ttcggaccaa cgaatatttg ggacaccgcc gctggtcatg ccgtggctgt
    16261 agctgccgga gcgcacgttc acgactggca gggtaaaccg ctggattaca ctccgcgcga
    16321 gtcgttcctg aatccggggt tcagagtatc tatttattaa ttaatttatg cagcaggtcc
    16381 atcacttgct gcacctcttc ctgagtaagc gccccgtctt tggcccattg cacgcgaccg
    16441 tctttatcca gcaccactac agcggaactt tcttcatcca gctgccaggc accgcgcgcg
    16501 acgccattgc tatcgacaat aaactgcgac cagggataaa gctttttatt actctccaga
    16561 ctgctgcgca caaacatgcc ggaacccgga attgcgtcgt cggtgttaac aatggtggtg
    16621 gtctggtaac gatcgtgcgg taactttgct gatttaatcg cttcaatcag cgtcgcgttt
    16681 ttctcttttg cagaggtgcg accagcaata tgttgcagta ctcgcacttt tcccactagc
    16741 tgcgcgctgt tccaggtttt gtagctaaac tgatctttat caagcaccaa ctcgccacga
    16801 tcggtaatgc caatcggcgg cactcgctga ccggtttcga actgatgtgc ggaagccatc
    16861 atcggcaaca gcaggcaggt gagtgccaga atcttgcgta gggtcatggt gtttccttct
    16921 tatgatatgc aggtgatccg accacttggg tctgagtttt aatcatatgt gctatttatc
    16981 gaatttcccg caagtgtgat gccagtttgc ggtcaagcgc acaaatcata tgaaaaatga
    17041 atgcttatac tgaagacggc gcttcggtaa aaagataatt ctgaataatt gtaaccttta
    17101 ggtaaaaaaa gttatacgcg gtggaaacat tgcccggata gtctatagtc actaagcatt
    17161 aaaatttgcg cctcataata gttgggccga ttgtggcacc gcacaggcgt aatactcagc
    17221 aggagataac aatgaaaatt ttccaacggt acaacccact tcaggtggcg aagtacgtaa
    17281 agatcctgtt ccgtggacgg ttatacatca aggacgttgg cgcttttgaa tttgataagg
    17341 gtaagattct tatcccaaaa gtgaaggata agctgcattt gtcagtgatg tccgaagtta
    17401 accgtcaggt tatgcgtctg caaacagaga tggcttaacc aaagtgctat gcagtaataa
    17461 gacggctcct gattcaggag ccgttgatgt ttctgggggt tacgcgacgc tttcttcttt
    17521 atctttagcg tcaggcaatt ttggcgaaag ggcggtggcc ttgctgtcga ttcgggtcac
    17581 cagcagctgg tcgatgcggt agttatcgat atccaccact tcaaatttgt agccggcgaa
    17641 tttcaccgaa tcggtgcgtt tcgggatctt acgcagcata aacatcataa agccgccgat
    17701 ggtttcgtag ttgcccgact gcgggaactc gtcaatatcc agcacgcgca tgacgtcgtc
    17761 aataggcgtg ccgccgtcta tcagccatga gttctcatca cgggcgacaa tttgctcttc
    17821 cagcccctga ccgaccagat cgcccatcag cgtggtcatc acgtcgttga gggtgatgat
    17881 ccccactacc agcgcgtact cgttcatgat caccgcgaag tcttcacctg cggttttaaa
    17941 actttccaac gcctctgaaa gggttaacgt atccggcaca atcagcgtat tgcgaatttg
    18001 tacgccgctg ttcagtgcca ggctttggtt agccagcacg cggttcagca ggtctttaga
    18061 atcgacatag ccgatgatgt ggtcaatatc ttcattacag acgagaaact tagagtgcgg
    18121 atgttccgcc accttattct tcagactttg ctcatcttcg tggagatcaa accaaatcac
    18181 gttttcacgc ggtgtcattg aagacggaac ggtacgggat tccagctcaa agacgttttc
    18241 aatcagctcg tgttcctgtt tacgtaacac gcccgccagc gcaccggctt ccactaccgc
    18301 gtagatgtca tcagaagtga tatcatcttt acgtaccatt ggcagtttga aaatacggaa
    18361 gattatgttc gccagtccgt tgaagaacca caccagcggg gtgcaaacgt acaggcagaa
    18421 gcgcatcggg ttgatgatac gcaaagccac agcttctggc gcaatcatac cgatgcgttt
    18481 cggggttaaa tccgcaaaca ggataaacat gccagtcact aacgagaaag agagaataaa
    18541 gctcagttgc tcagagagtt ctgccgacat atagcgggag aacaggctgt gaaaagctgg
    18601 agaaaatgcc gcatcaccga cgataccgcc gagaatcgcc actgcgttca gaccgatctg
    18661 gaccacggta aagaacatgc cgggattttc ctgcatattc agaacgcgtt gggcatttat
    18721 attgccttca tcagccagca gtttaagttt gattttgcgt gaggcggcaa gcgagatctc
    18781 ggacatcgag aagaacgcac ttacagcgat caaacagagt atgactaaaa tactgtttaa
    18841 catatcttat ccgacctttc aggccagatc ctcggaaggg gaagttgatt aatttgtgtg
    18901 aaatacacat tgaaagccga ttcgaagaaa gtgaaccggc attttcagtg gctagtatag
    18961 cgtaaggtac tgtaaagccg ccagagggtt aaaattcaga cagctgaaaa atgaaaaact
    19021 gcctgatgcg ctatgccgaa caggcctacg cagtccccgc aatatattga atttggacga
    19081 ttttgtaggc ctggcaaggc gtttacgccg tatccggcat aaacaaaatg cactttatca
    19141 acaatctaca tttgtacccg taacgctatg cttccggcgg cagacagacg ccaattccgc
    19201 caattccaca gtaaccatac gggtttttat gcagatattg ctggtggtca tcttcggcat
    19261 aataaaacgg tgtggcgtta gcgatttccg tggtgatgcg acgatcgtca tcggcggcaa
    19321 gcatcgccgc ctgaaaacgt tccagactgg cgcgagcggc ggcatcctgt tctggggtca
    19381 gcggataaat cgctgaacga tactgcgtgc cgtggtcatt gccctgacgc attccctggg
    19441 cgggatcgtg attctcccaa aatacttgta gcaactgctc atagctgatg acggatggat
    19501 cgtaaacaat gcgcaccgct tcggcatggc cagtatcgcc ggagcacact tcccgataag
    19561 tcggatttgg cgtatagccg ccggtatagc ctgcggcggt gctgtaaacg ccgggtaact
    19621 gccagaacag acgctccaca ccccagaaac aacccatcgc aaaaatggca atctccattc
    19681 cgtcaggtac attggtcatt gagtgaccgt tgaccgcatg cagtgtggct acgggcatcg
    19741 gggtgttacg tccaggcagg gcatcggcgg gggaaaccag atgcttttta tcaaataaac
    19801 tcatggtgtc gctctcccga aaatcggtca ttggggttaa ggttgtaaca agaggcgtat
    19861 ttgcacacaa taaccaccgt gaataggtct aaagtaaaac ataagaaata ttttgggttt
    19921 agtctgcttt ttaatccata ttactggatt tttgttaagc cgtttaacgg cgttccaggg
    19981 gcaggaaaaa aggatattca ggagaaaatg tgcgctatat ccgacagtta tgctgtgtaa
    20041 gcttactctg cttaagcgga tctgccgtcg ccgcgaacgt ccgtctacag gtcgaggggt
    20101 tatcgggaca gctggaaaag aacgttcgcg cgcagctttc tacgattgaa agtgatgaag
    20161 tgacgccaga ccgtcgcttt cgcgcacgcg tcgatgatgc catccgcgaa ggtctgaaag
    20221 cgctgggcta ttaccagccg accattgaat ttgatctccg tccaccgcca aagaaagggc
    20281 ggcaggtatt gatcgccaaa gtcacgccag gcgtgccggt gttaattggc ggcaccgatg
    20341 tggtattgcg cggcggcgcg cggaccgata aagactattt gaaattgctc gatactcgcc
    20401 cggctattgg tacggtactg aaccagggcg attatgaaaa tttcaaaaag tccttaacca
    20461 gcattgcgtt gcgtaaaggt tatttcgata gcgaatttac caaagcgcag ctgggcattg
    20521 cgctcggcct gcataaagcc ttctgggata ttgattataa cagtggcgaa cgttaccgct
    20581 ttgggcatgt gacctttgaa ggatcacaaa tccgcgatga atacctgcaa aatctggtgc
    20641 cgtttaaaga gggcgatgag tacgaatcga aagatctggc agaactgaac cgtcgacttt
    20701 ctgctaccgg ctggtttaac tcggtggtgg tggctccaca atttgataaa gcgcgcgaaa
    20761 cgaaagtatt accattgacg ggcgtggttt cgccgcgaac agaaaacacc atcgaaaccg
    20821 gcgtcggtta ctctacggac gtgggaccgc gcgtgaaagc gacgtggaaa aagccgtgga
    20881 tgaactctta cggtcacagt ttgaccacca gtaccagtat ttccgcgccg gaacagatcc
    20941 tcgacttcag ctataaaatg ccgctgctga agaatccgct ggaacaatat tatttggtgc
    21001 agggcggttt taagcgcact gacctgaacg ataccgagtc tgactccact acgctggtgg
    21061 cttctcgcta ctgggatctc tccagcggct ggcagcgtgc cattaacctg cgctggagtt
    21121 tcgaccactt tacccagggt gaaattacca acaccacgat gctgttttat cctggggtga
    21181 tgattagccg cacgcgttct cgtggtggcc tgatgccaac ctggggcgac tcgcaacgct
    21241 actctatcga ctactccaac acggcctggg gctcggatgt tgatttctcc gttttccagg
    21301 cgcagaacgt ctggatccgc acactgtacg atcgccatcg ttttgtgaca cgcggcacgc
    21361 tgggctggat tgaaacgggt gatttcgaca aagtaccgcc ggatctgcgt ttcttcgccg
    21421 ggggcgatcg cagtattcgc ggctacaaat acaaatctat cgccccgaaa tacgccaacg
    21481 gtgacctgaa aggggcctcg aagctgataa ccggatcgct ggaataccag tacaacgtga
    21541 ccggaaaatg gtggggcgcg gtgtttgtcg atagcggcga agcggtaagc gatattcgcc
    21601 gcagcgactt taaaaccggt accggggtcg gcgtgcgctg ggaatcgccg gtcgggccaa
    21661 tcaaactcga ttttgccgta ccggtcgcgg ataaagacga acacgggtta cagttttaca
    21721 tcggtctggg gccagaatta tgagtttatg gaaaaaaatc agcctcggcg tggttatcgt
    21781 tatcttactg ttgctgggat cggtggcgtt tctggtgggc accaccagcg gcctgcatct
    21841 ggtatttaaa gcggcggatc gctgggtgcc aggactggat attggcaagg tcaccggcgg
    21901 ctggcgtgat ctcaccttgt ctgacgttcg ttatgagcag ccaggcgtgg cggtaaaagc
    21961 gggcaatctg catctggcgg tcgggcttga gtgcctgtgg aacagtagcg tttgtattaa
    22021 tgatctggcg ctgaaagaca ttcaggtcaa catcgacagt aaaaaaatgc ctccttctga
    22081 acaggttgaa gaagaggaag atagcggtcc gttggatctc tccatgccgt atcccattac
    22141 cctgacacgg gtggcgctgg acaacgtcaa catcaagatt gatgacacca cggtatcggt
    22201 gatggacttc acctccggcc tgaactggca ggagaaaacc ctgaccctga aaccgacatc
    22261 gctgaaaggc ctgttgattg ctttgccgaa agtggcggaa gcggcgcagg aagaagtggt
    22321 cgaaccgaaa attgaaaatc cacagccgga ggaaaagccg ctcggcgaaa cgctgaaaga
    22381 tctcttttct cgcccggtat tgccggaaat gaccgacgtg catctgccgc tcaacctgaa
    22441 cattgaagag tttaagggcg agcaactgcg cgtgacgggc gacacggaca tcaccgtgcg
    22501 caccatgctg ctgaaagtga gcagtatcga cggcaatacc aaactggacg cgctggatat
    22561 cgactccaac caggggatcg tcaacgccag cggcacggcg cagctgtcag acaactggcc
    22621 ggtggatatc accctcaaca gcacactgaa cgtggagccg ttgaaaggtg aaaaggtgaa
    22681 gctgaaagtg ggcggtgcgc tgcgcgaaca gctggagata ggcgttaacc tttccggtcc
    22741 ggtggatatg gatttacgcg cccagacgcg actggcggaa gccggattgc cgctcaacgt
    22801 ggaagtgaac agcaaacagc tttactggcc gttcactggt gagaagcagt atcaggcgga
    22861 tgatctgaaa ctgaaactta ccggcaaaat gaccgattat gcgatctcta tgcgcacggc
    22921 tgttaagggc caggagatcc cgccagccac cattaccctt gatgccaaag gtaatgaaca
    22981 gcaggtcaat ctcgacaaac ttaccgtcgc ggcgctggaa gggaaaacgg aacttaaggc
    23041 gttgctcgac tggcagcagg ccattagttg gcgcggtgag ctaacgctta acggtattaa
    23101 caccgccaaa gagatcccgg agtggccgtc gaaactcaat ggcttgatta aaacccgcgg
    23161 tagcctgtac ggcggcacct ggcagatgga ggtgccagaa ctgaagctga ccggtaacgt
    23221 caaacagaac aaagtgaacg ttgacggcac gctgaaaggc aacagttata tgcagtggat
    23281 gatcccaggg ctgcatctgg aactggggcc aaacagtgcc gaagtgaaag gcgagctggg
    23341 ggtaaaagat ctcaatcttg atgccaccat caacgcgccg gggctggata acgcgctgcc
    23401 ggggcttggc ggtacagcga aagggttggt gaaagtacgc ggcacggtgg aagcgccaca
    23461 actactggca gatatcaccg cgcgcggcct gcgctggcag gaactttccg tggcgcaggt
    23521 tcgcgtggaa ggcgatatca aatccaccga ccagattgcg ggtaaactcg acgtacgcgt
    23581 tgagcaaatt tcgcagccgg atgtaaatat caacctcgtc accctgaatg ccaaaggcag
    23641 cgaaaagcag cacgagctac agttgcggat tcagggcgag cctgtctccg ggcagcttaa
    23701 tctggcagga agttttgatc gcaaagaaga acgctggaag ggaactctta gcaatacccg
    23761 cttccagacg ccggtcggcc cgtggtcgct gacccgcgat attgcgctgg attaccgcaa
    23821 taaggagcaa aaaatcagca tcgggccaca ctgctggctt aacccgaatg cggaactgtg
    23881 cgtgccgcaa actatcgatg cgggtgccga agggcgtgcg gtggtgaatc tcaaccgctt
    23941 cgacctggcc atgctgaaac catttatgcc agaaaccact caggccagcg gtatcttcac
    24001 gggtaaagcg gacgttgcct gggacaccac gaaagagggg ctgccgcagg gcagtatcac
    24061 cctttcgggg cgtaatgtgc aggtgacgca aaccgtcaac gatgcggcgc tgcccgtggc
    24121 gtttcagacg ctgaatctga cggcggaatt gcgtaacaac cgtgccgaat tgggctggac
    24181 catccgcctg accaataacg gccagtttga tggacaggtg caggtgaccg atccgcaagg
    24241 ccgccgtaat cttggtggca acgtcaatat ccgtaacttc aaccttgcga tgataaaccc
    24301 catctttacc cgtggggaaa aagcagcggg gatggtgagt gccaacttgc gtctgggtgg
    24361 tgatgtgcaa agcccgcagt tgtttggtca gcttcaggtt acgggtgtgg atatcgacgg
    24421 caactttatg ccgtttgata tgcaaccgag ccagcttgcg gtcaacttta acggtatgcg
    24481 ctcgacgctt gccggtacag tacggaccca gcagggtgaa atctacctga acggtgatgc
    24541 cgactggagc cagattgaaa actggcgggc gcgggtaacg gcgaaaggca gtaaagtgcg
    24601 gatcaccgtg ccgccgatgg tacgaatgga tgtatcgcca gatgttgtat tcgaggctac
    24661 accaaacctg tttaccctcg atggtcgcgt ggatgtcccg tgggcgcgca tcgtggtgca
    24721 tgagctgccg gaaagcgcgg tgggcgtctc cagcgatgtg gtaatgctta acgataacct
    24781 gcaaccggaa gaggcaaaaa cggcgtcgat tccgattaac agcaacctga ttgtccacgt
    24841 cggcaacaat gtgcgcattg acgcctttgg cctgaaagcg cggctgacgg gcgatctcaa
    24901 cgtcgttcag gacaaacaag ggctgggcct gaacgggcag atcaacatcc ctgaagggcg
    24961 cttccatgcc tatggtcagg atctgattgt gcgtaagggt gagttactgt tctctggtcc
    25021 gccggatcaa ccgtatctta atatcgaagc tattcgtaac ccggatgcta cagaagacga
    25081 cgtaatcgcc ggagttcgcg tcactggtct ggcggacgaa ccgaaagcgg agatcttctc
    25141 tgacccggcc atgtcgcaac aggctgcctt gtcttatttg ctacgtggac aagggctgga
    25201 gagcgatcag agcgacagtg cggcgatgac ctcgatgctg attggtctgg gggttgcaca
    25261 aagtggtcag attgtgggta aaatcggcga gacgtttggc gtaagcaatt tagcgctcga
    25321 cacccaggga gtaggcgact cctcccaggt ggtggtcagc ggctatgtat tgccaggtct
    25381 gcaagtgaaa tacggcgtgg gtatatttga ctctatagca acactcacgt tacgttatcg
    25441 cctgatgcct aagctatatc tggaagccgt gtctggtgta gaccaggcac tggatttgct
    25501 ctatcagttc gagttttagc aatgcgaata tttgtctacg gcagtttacg ccacaaacaa
    25561 ggcaacagtc actggatgac caatgcccag ttactgggcg atttcagtat cgataactac
    25621 cagttgtata gcctgggcca ctatccaggc gcagttccgg ggaacggaac agtacacggt
    25681 gaagtttatc gtattgataa cgccacgctg gccgaacttg atgccttgcg caccaggggc
    25741 ggtgaatacg cgcgccagtt gattcagacg ccgtacggga gtgcatggat gtacgtttat
    25801 caacgacccg tcgatggatt aaagctaatt gaaagcggcg actggttaga cagggataag
    25861 taataataaa agcacgccac cttcgggtgg cataaggggg tagcgcgtat cgctatcccc
    25921 ctttatatat caaatggtta cgagttcaaa gtggttcgga atagtgccat ttagtccccg
    25981 tttatgggca aaatctggac aactccgcaa ccggattgaa ccttaccgcg tcctggagat
    26041 aatcaggtgc aaagtgcgca taggtcattg tctgctgaat tgtgctgtgc cctaaaatac
    26101 gctgaagggt aataatgttc cctccattaa tcatgaaatg agttgcgaat gtgtgccgta
    26161 aaacatgcac agactgaccg actggtaaat caggccgcac agtccttatt gcacttctga
    26221 ctatcgcata actggggttc attaatcttc ccgtcgcttt gactttaatc agagattcaa
    26281 gagctttagc tataggaata acccgccgcg atccattctt tgtctccatg aaaataacct
    26341 tcccaccaat tatgtgttca cctttaatat tggcaacttc accccagcg
//
LOCUS       DAZGHF010000066        26327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGHF010000066 DAZGHF010000000
VERSION     DAZGHF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.066"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(114..197)
                     /gene="yohP"
                     /locus_tag="ACHK3A_002797"
     CDS             complement(114..197)
                     /gene="yohP"
                     /locus_tag="ACHK3A_002797"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO8740854.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            421..1857
                     /gene="mdtQ"
                     /locus_tag="ACHK3A_002798"
     CDS             421..1857
                     /gene="mdtQ"
                     /locus_tag="ACHK3A_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO8740855.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQHTPAVQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPVMQVAEQRIQLAEAQAKAIATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVDAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1910..2671
                     /gene="yohF"
                     /locus_tag="ACHK3A_002799"
     CDS             1910..2671
                     /gene="yohF"
                     /locus_tag="ACHK3A_002799"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8740856.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGARAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVRPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2790..3368)
                     /gene="yohD"
                     /locus_tag="ACHK3A_002800"
     CDS             complement(2790..3368)
                     /gene="yohD"
                     /locus_tag="ACHK3A_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO8740857.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILAVVLVVGVRWWLKRRGKKKPDNQA"
     gene            3538..4125
                     /gene="yohC"
                     /locus_tag="ACHK3A_002801"
     CDS             3538..4125
                     /gene="yohC"
                     /locus_tag="ACHK3A_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO8740858.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4299..5231
                     /gene="pbpG"
                     /locus_tag="ACHK3A_002802"
     CDS             4299..5231
                     /gene="pbpG"
                     /locus_tag="ACHK3A_002802"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO8740859.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5270..6985)
                     /gene="dld"
                     /locus_tag="ACHK3A_002803"
     CDS             complement(5270..6985)
                     /gene="dld"
                     /locus_tag="ACHK3A_002803"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO8740860.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEYLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGVGKTSKRKN
                     WQEVE"
     gene            7181..9478
                     /gene="bglX"
                     /locus_tag="ACHK3A_002804"
     CDS             7181..9478
                     /gene="bglX"
                     /locus_tag="ACHK3A_002804"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO8740861.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATAKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9627..10544
                     /gene="osmF"
                     /locus_tag="ACHK3A_002805"
     CDS             9627..10544
                     /gene="osmF"
                     /locus_tag="ACHK3A_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO8740862.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10551..11708
                     /gene="yehY"
                     /locus_tag="ACHK3A_002806"
     CDS             10551..11708
                     /gene="yehY"
                     /locus_tag="ACHK3A_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO8740863.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACVIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHLLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGISGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11701..12627
                     /gene="yehX"
                     /locus_tag="ACHK3A_002807"
     CDS             11701..12627
                     /gene="yehX"
                     /locus_tag="ACHK3A_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO8740864.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLVEA"
     gene            12632..13363
                     /gene="yehW"
                     /locus_tag="ACHK3A_002808"
     CDS             12632..13363
                     /gene="yehW"
                     /locus_tag="ACHK3A_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO8740865.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKSMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13344..13451)
                     /gene="yohO"
                     /locus_tag="ACHK3A_002809"
     CDS             complement(13344..13451)
                     /gene="yohO"
                     /locus_tag="ACHK3A_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO8740866.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(13511..14242)
                     /gene="mlrA"
                     /locus_tag="ACHK3A_002810"
     CDS             complement(13511..14242)
                     /gene="mlrA"
                     /locus_tag="ACHK3A_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO8740867.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14464..16149
                     /gene="btsS"
                     /locus_tag="ACHK3A_002811"
     CDS             14464..16149
                     /gene="btsS"
                     /locus_tag="ACHK3A_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO8740868.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDCYTRITLRLPWRDEA"
     gene            16146..16865
                     /gene="btsR"
                     /locus_tag="ACHK3A_002812"
                     /gene_synonym="yehT"
     CDS             16146..16865
                     /gene="btsR"
                     /locus_tag="ACHK3A_002812"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO8740869.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16912..17382
                     /gene="yehS"
                     /locus_tag="ACHK3A_002813"
     CDS             16912..17382
                     /gene="yehS"
                     /locus_tag="ACHK3A_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO8740870.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17424..17885)
                     /gene="yehR"
                     /locus_tag="ACHK3A_002814"
     CDS             complement(17424..17885)
                     /gene="yehR"
                     /locus_tag="ACHK3A_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO8740871.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(18010..20013)
                     /gene="yehQ"
                     /locus_tag="ACHK3A_002815"
     CDS             complement(18010..20013)
                     /gene="yehQ"
                     /locus_tag="ACHK3A_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO8740872.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIDGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGKCLCQLAALTSRLNSAQEIARRDSVGEVPPMPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVEGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEHSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(20010..21146)
                     /gene="yehP"
                     /locus_tag="ACHK3A_002816"
     CDS             complement(20010..21146)
                     /gene="yehP"
                     /locus_tag="ACHK3A_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO8740873.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGDSNLTTPEWINSIHTLFPQRVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSDQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(21139..23418)
                     /gene="yehM"
                     /locus_tag="ACHK3A_002817"
     CDS             complement(21139..23418)
                     /gene="yehM"
                     /locus_tag="ACHK3A_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO8740874.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPYAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREDFMARWIGWAMQQNNGDVLVVCGGWHASALAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWRWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISFPAE
                     LTLNRFTPDGLAQSQVLHRLVILEIPGVVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFNQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLVQLSPTQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(23429..24517)
                     /gene="yehL"
                     /locus_tag="ACHK3A_002818"
     CDS             complement(23429..24517)
                     /gene="yehL"
                     /locus_tag="ACHK3A_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO8740875.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDHAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            complement(25665..26018)
                     /locus_tag="ACHK3A_002819"
     CDS             complement(25665..26018)
                     /locus_tag="ACHK3A_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740876.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRKLQNIFNQ
                     LIEYYFIKGGSGFPP"
     gene            complement(26080..>26327)
                     /locus_tag="ACHK3A_002820"
     CDS             complement(26080..>26327)
                     /locus_tag="ACHK3A_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740877.1"
                     /translation="LMGMRYKGWESGYEDAFVYGLGEYRLVLKFSPGFNHYNVDSKAL
                     MSFRSLRVYRDNKSVTFAELDVFDLSEALSAPDVIFH"
BASE COUNT         6198 a         6648 c         7119 g         6362 t
ORIGIN      
        1 ccccgcgctc cttgcgccag tgattatatg aagcaatgca catgacagga gcgcaacagg
       61 atgagagact gaaatattct cgttattttc ggatctgatg acattaatta attttaaaat
      121 atcatcttaa atacgccagt caccaccagt agcccaatca ggaaaataat caatacagcc
      181 cagagtataa ttttcattgg cgttccttta tttgtgagtg ctgaataagt ataggaaaaa
      241 acgaagccct ttgccgctat ccataaacga ttaagataaa tcggaagata tcattcactt
      301 ggtgtatatt tttctggata tgtcgtttta ttgccactga tgtattgcca cgcgaaacta
      361 aaaaatatat cgcaacacac ggtaatctcc gcgcaccttt tcttacctga ttattccccc
      421 atgaatcgtg attcctttta tccagccatc gcctgttttc cgctgttact gatgctggcc
      481 gggtgtgcgc ctatgcatga aacccgccag gcgttaagcc agcacacgcc cgctgtgcaa
      541 gttgacaccg cattacccac ggcgctgaaa aatggttggc cagacagcca atggtggctg
      601 gagtatcacg ataatcaact cacctcatta attaataacg cactacagaa cgcaccggtt
      661 atgcaggtgg ctgaacagcg cattcagctg gctgaagccc aggcgaaggc aattgctact
      721 caggatggtc cgcagctcga cttttctgcg gatatggagc ggcaaaaaat gtcggcagaa
      781 ggcttaatgg ggccgtttgc tctgaacgat ccggccgcag gtacgaccgg tccgtggtac
      841 accaacggta cttttggctt aacggcgggc tggcatctcg atatctgggg aaagaatcgg
      901 gcggaggtaa ctgctcgcct gggtacggtt aaagcacgag cggcggaacg cgagcaaacc
      961 cgccaattgc tggctggcag cgtagcccgc ctgtactggg agtggcaaac ccaggcggcg
     1021 ttaaacacgg tcttgcagca aatcgaaaaa gagcagaaca ccattatcgc caccgatcgc
     1081 cagctatatc agaacgggat tacttcttca gttgaaggtg tggaaaccga tattaatgcc
     1141 agcaaaaccc ggcagcagct caacgatgtc gcggggaaaa tgaaaattat tgaggcacgg
     1201 ttaagtgcac ttacaaatca ccagacaaag gcattgaagc ttaatccggt cgcgttgccg
     1261 aaagtggcaa gccagcttcc tgatgaactg gggtactcct tactggcccg gcgggcagat
     1321 ttgcaggcgg cgcactggta cgttgagtca tcgttaagca ctattgatgc ggcaaaagcg
     1381 gcattttatc ctgacatcaa cctgatggcc ttcctgcaac aggatgcgtt gcacttaagc
     1441 gatctgttcc gtcattccgc gcagcaaatg ggcgttacgg caggcctgac gctacccatt
     1501 ttcgatagtg gtcgtcttaa cgccaacctc gatatcgcaa aagccgaaag caacttgtct
     1561 atcgccagct acaacaaagc ggtggttgac gcggtgaatg acgtggcgcg ggcagccagt
     1621 caggttcaga cactggcgga gaaaaaccag catcaggcgc aaattgagcg cgatgccttg
     1681 cgtgtagtag gtcttgcaca ggcgcgcttt aacgcgggca tcattgccgg ttcccgcgtc
     1741 agcgaagcca gaatcccggc gcttcgcgag cgggccaatg gcctgttatt gcaagggcag
     1801 tggctggatg cctccattca actcactggt gcgttgggcg ggggttacaa acgctgatga
     1861 tatgcgcctt ctatacttaa cgtttattca gcgttaagtg gagaactcga tggcacaggt
     1921 tgcgattatt accgcctccg attcggggat cggcaaagag tgcgcgttat tactggcgca
     1981 gcaggggttt gatattggca ttacctggca ctctgatgaa gaaggggcaa aagatactgc
     2041 gcgtgaggtg gttagccacg gcgcacgtgc ggagatcgtg cagctggatc tcggtaaact
     2101 gccagaaggt gcgcaggcgc tggagaaact cattcaacga ctggggcgca tcgatgtgct
     2161 ggtgaataat gcaggtgcaa tgaccaaagc gccgtttctt gatatggctt ttgatgagtg
     2221 gcgcaagatt tttaccgttg atgtcgacgg tgcattctta tgctcgcaaa ttgcggctcg
     2281 tcagatggtg aaacaagggc agggcggtcg catcatcaac attacgtcgg tgcatgaaca
     2341 tacgccgctg ccggatgcca gcgcttacac agcagctaaa catgcgctcg gcgggttaac
     2401 caaagcaatg gcgctggagt tggtcaggca taaaattttg gtgaacgcag tcgcacctgg
     2461 ggcgatcgcc acgccaatga acggcatgga tgatagcgat gtgaggcccg acgcggagcc
     2521 ttcgattccc ttgcgacgtt ttggcacaac gcatgagatt gccagcctgg tggtatggct
     2581 ttgttcggag ggcgcaaatt acaccaccgg gcagtcgttg atagtggatg gcggctttat
     2641 gttggcgaat ccgcagttca acccagaata gcgcagtgag gtgcgtggtt atttcaatgg
     2701 gttaggccag ataagacacg ccagcgtcgc atcaggcatg gaacatccgg tgccggatgt
     2761 ggcgtgaatg cttatctgtg gcaactgctt tacgcctgat tatccggctt tttcttcccg
     2821 cgtcgtttca gccaccagcg cacgcctacc accagaacca cggccagaat caaccagacc
     2881 cagtgcttca aatgctggtc gagattgtgc aaccacggcg caatcacctg accaccagca
     2941 taaccgatag tggtaaaaat caacgcccag gcaaatgcgc cgagaatatt cagcggcaga
     3001 aagattttcg gcggcagctg gctggcacca atcagcgtcg ggccaatcac cctaaagcca
     3061 tacataaaac gcgtaccaat gacaaacaga tacggatggc gttggataag tttctgcgcc
     3121 cgctcaattt tatcctgatg tttcgagaaa cggcgtaata gcttgccgcc aaagcgccgc
     3181 ccgcacagat aaagcacctg gtcgccaatc atgccgccaa gcgccacaga aagtaccacc
     3241 agcgggaact ttaatagccc ctgatgtgcc gcaacgcctc ccagcaaagt cacggtttca
     3301 ccttccgcca ggctaccgat caccagcgcg gcataaccat attgtgagat gagtgtattg
     3361 agatccatag gccagtctgt ctccctttgc ttgcctgtaa gcatacaccc tgtgaatagt
     3421 tcacgctgaa aaaggacgta catcagacgg tgcaaattac atgagcgaac aaaaaataat
     3481 cacaagctga cttatacttg aactgtcagg ttcatgaact cacaacaagg gggcggtatg
     3541 agccatgtct ggggactgtt ttcccatccc gatcgtgaaa tgcaggtgat taatcgcgaa
     3601 aacgaaacga tttctcacca ttacacccac cacgttttgc tgatggcggc gattccggtg
     3661 atttgcgcct tcattggcac tacacagatt ggctggaatt ttggcgatgg cactatcctg
     3721 aagttatcct ggtttaccgg actggcgctg gctgtcttgt tttacggcgt tatgctggct
     3781 ggggttgcgg tgatggggcg ggtcatctgg tggatggcgc gtaattatcc gcagcgtccg
     3841 tcacttgcgc actgcatggt cttcgcgggc tacgttgcaa ctccgctgtt tttaagcggt
     3901 ctggtggcgc tttacccgct ggtctggttg tgtgcgctgg tcggcacggt ggcgctgttt
     3961 tacaccggct acctgctgta tctcggtatt ccgagcttcc tgaatatcaa caaagaggaa
     4021 ggacttagct tctccagttc gacactggcg ataggcgtgc tggtgctgga agtgctgctc
     4081 gcccttaccg ttatactatg gggctacggt tatcgtttgt tctagaaagt tcttttgcgc
     4141 tgcattgctg gcgtaaatgt cagcaatgca gcatttcctc acgattcttc tttgacgatc
     4201 gcactttttg ctcgttatga tggcgtcgct agcctcagta aatccttact ccggcggtgc
     4261 gcaacccgtg cgcgtgaacc actatctgaa tgctcatcat gccgaaattt cgagtttctt
     4321 tatttagcct ggccctgatg ctggctgtgc ctttggcacc gcaggccgtt gcgaaaacgg
     4381 cagccgctac caccgcttca caaccggaaa ttgcctccgg tagcgcgatg attgttgatc
     4441 tgaataccaa caaagtgatc tattcgaacc acccggatct ggtgcgtccg attgcgtcta
     4501 tcagcaaatt aatgaccgcg atggttgtgc tggatgcacg actgccgctg gatgaaaaac
     4561 taaaagtgga tatcagccag acgccggaga tgaaaggggt ctattcgcgc gtacgactga
     4621 acagcgaaat cagccgtaaa gatatgctgt tgctggcgct gatgtcttca gaaaaccgcg
     4681 cggcggcaag ccttgcgcac cattatcccg gtggttacaa agcctttatt aaggcgatga
     4741 atgcgaaagc gaaatcgctc ggaatgaaca acacgcgctt tgttgaaccg accggattgt
     4801 cggtgcataa cgtttcaact gcccgtgact taaccaaact gctcattgcc agcaaacaat
     4861 atccgttgat tggtcagtta agtactacca gggaagatat ggcaaccttc tctaacccga
     4921 cgtatacgct gccgttccgc aatactaacc atctggtgta tcgcgataac tggaatattc
     4981 agttaaccaa aacgggcttt accaatgcgg cgggccattg tctggtgatg cgtacggtta
     5041 tcaataataa accggtggcg ctggtagtga tggacgcgtt tggcaaatat acccattttg
     5101 ccgatgccag ccgcctgcgt acctggattg agaccggtaa agtgatgcct gttccggcag
     5161 cagcgttgag ctataaaaaa caaaaagccg cccaaatggc ggcggcgggg cagacggcac
     5221 agaacgatta aatgaatgac ggatggcgat actctgccat ccgtaatttt tactccactt
     5281 cctgccagtt tttccgctta ctggttttac caacccccgg attcatactg ttggtcggat
     5341 cgttctcgcg ataaaacttc tgcaacgttt ccggtgcttt atacaaatga ccgacgttat
     5401 gctcggcagg gtattgcgcg ccgcgctgct gtagcagttc cagcatctgt tctttcaacg
     5461 catgcacatc cacgcctttt ttaacgatgt aatcctgatg gaagacatag cacataaaat
     5521 ggccgtaata gagcttgtgc accagctggc tgtcgatctc cggcggtaaa tactcatacc
     5581 actcggtgtc gttacgccgc agagcgatat ccagtgccag aatgtcttcg acttcatcgg
     5641 aatgcaccgc ctgataacga attgctgcgc ccgcagcggc gaaacggtgt aaaaacgctt
     5701 tgctgccttc ctccggcgta cagacaaaga aatcgccttc ggcctgtttg aaataatcca
     5761 ccagccacga tttggcttca ccgacgccat cgcccgccat ttttaacagc agatgatgtt
     5821 cgtatttatc gcgccagttt ttcatgcgcg gcggtaaatg gctggggaac aggtgaccga
     5881 atttttgcat cgcacggtcg gtaaaatgcg gacggaagaa tttcactttc tccagcatcg
     5941 catcggtgcg ccccttgaga ttaaagaaga acggcatctt gtcggtgcct aacttatcaa
     6001 tcatcaggaa ggtgtctttg ccgtattttt ccgctatatc gtagatatcc cggtgcatat
     6061 attccccggc aaccggcaga ttttcgaaat tagccagaat atgacggcgg atttcggtca
     6121 gtacttccgg ctggttggtg ccgatataaa acacctgctg atttttttcc gcttcgaagg
     6181 tatcaagacg taccgcaaag acggccagct tcccggcgca accagaagat tcaaataacc
     6241 gatccggatc ggcgttataa cgtgcgggcg tgtcggcttc aatatcacga acgcggtgga
     6301 catagtcata atcgtgggcg tgacgaccat cgtggcgcac atcgtcatct ttgatgcgat
     6361 cgtcatccag cttgctaagg atctgttccg gcgtttcgcc cagatcaatc cccagatggt
     6421 taaccagcgt cagcttgcca tcttcattta tacgcgcgaa taacgacatt tcggtatacg
     6481 ccgggccacg ttgcaccagc gagccgccgg agttgttaca aataccgccg atgaccgatg
     6541 cgcctataca cgatgatcca atcaccgagt gcggttcgcg tcccagcggt ttgagggctt
     6601 tttccagcga ataaagcgtg gtgcccggat aggccagcac ctgttcgccc ttgccgagaa
     6661 cgtgcagctt gtcgagacgc agggtgctga tgatcacgat atcgcgatca tagtcgttac
     6721 cgtttggcgt cgatccttcg gtcaggcctg tattggcagc ctgcatcaga ataattttgt
     6781 cggcggtgac gcaggctttc agcacccgcc acaattccag tagtgagcca gggaaaacga
     6841 cagccagtgc gtcgccctga ccagaacgga agcccttgcg atagcgggcc gtttttgcgg
     6901 gatcggtgag caggtgtgaa tgaccgacca gacgggcaag ttcattcaaa aaggctttat
     6961 tatcagttgt tgtcatggaa gacattttcc actccttgtg gtggcgaaaa aatatcacaa
     7021 actagaatag cgccttgtat gacaattcag cgagtgaata ttttgaaaat tcagacaata
     7081 aactgagcat tctttttctc tctccatcat gcttatggca aactacggtt tttgcaccat
     7141 tttcgctgcc acgtcgggca acaaaggaag aaaaatccat atgaaatggc tatgttcagt
     7201 aggaatcgcg gtaagtctgg ccctgcagcc agcactggcg gatgatttat tcggtaacca
     7261 tcccttaacg cctgaagcgc gggatgcgtt cgtcaccgaa ctgcttaaga aaatgacggt
     7321 tgatgagaaa attggtcagc tgcgcttaat cagcgtcggc ccggataatc cgaaagaggc
     7381 gatccgcgag atgatcaaag acgggcaggt tggggcgatt ttcaacaccg taacccgtca
     7441 ggatatccgc gccatgcagg atcaggtgat ggaattaagc cgcctgaaaa ttcctctttt
     7501 ctttgcttac gacgtgctgc acggtcagcg cacggtgttc ccgattagcc tcggtctggc
     7561 ctcgtctttt aacctcgatg cggtgaaaac ggtcggacgt gtctctgctt atgaagcggc
     7621 agatgatggc ctgaatatga cctgggcacc gatggtcgat gtctcgcgcg atccgcgctg
     7681 gggacgtgct tccgaaggtt ttggcgaaga tacgtatctc acttcaacaa tgggtaaaac
     7741 catggtggaa gcgatgcagg gtaaaagccc ggcagatcgt tactcggtga tgaccagcgt
     7801 caaacacttt gccgcatacg gcgcggtaga aggcggtaaa gagtacaaca ccgtcgatat
     7861 gagtccgcag cgcctgttta atgattatat gccgccgtac aaagcggggc tggacgcagg
     7921 cagcggcgcg gtgatggtgg cgctgaactc gctgaacggt acgccagcca cctccgactc
     7981 ctggctgctg aaagatgttc tgcgcgacca gtggggcttt aaaggcatca ccgtttccga
     8041 tcacggcgca atcaaagagc tgattaaaca tggcacggcg gcagacccgg aagatgcggt
     8101 gcgcgtggcg ctgaaatccg gcatcaacat gagtatgagc gacgagtatt actcgaagta
     8161 tctgcctggg ttgatcaaat ccggcaaagt gacgatggaa gagctggatg acgctgcccg
     8221 tcatgtactg aacgttaaat atgatatggg gttgtttaac gacccataca gccatttggg
     8281 gccgaaagag tctgacccgg tggataccaa tgccgaaagc cgcctgcacc gcaaagaagc
     8341 gcgtgaagtg gcacgcgaaa gcctggtgtt gctgaaaaac cgtctcgaaa cgttaccgct
     8401 gaaaaaatca gccaccattg cggtggttgg cccgctggca gacagcaagc gtgacgtgat
     8461 gggaagctgg tccgcagccg gtgtcgccga tcaatctgtt actgtgctaa cagggattaa
     8521 aaacgccgtc ggtgaaaacg gtaaagtgct gtatgccaaa ggggcgaacg ttaccagtga
     8581 caaaggcatt atcgatttcc tgaatcagta tgaagaagcg gtcaaagtcg atccgcgttc
     8641 gccgcaagag atgattgatg aagcggtgca gaccgcgaaa caatctgatg tggtggtggc
     8701 tgtagtcggt gaagcacagg ggatggcgca cgaggcttcc agccgtaccg atatcactat
     8761 tccgcaaagc caacgtgact tgattgcggc gctgaaagcc accgctaaac cgctggtgct
     8821 ggtgctgatg aacgggcgtc cgctggcgct ggtgaaagaa gatcagcagg cggatgcgat
     8881 tctggaaacc tggtttgcgg ggactgaagg cggtaatgca attgccgatg tattgtttgg
     8941 tgattacaac ccgtccggca agctgccgat gtccttcccg cgttctgtcg ggcagatccc
     9001 ggtgtactac agccatctga ataccggtcg cccgtataac gccgataagc cgaacaaata
     9061 cacgtcgcgt tattttgatg aagctaacgg ggcgctgtat ccgttcggct atggtctgag
     9121 ctacaccact ttcaccgtct ctgatgtgaa actttccgcg ccgaccatga agcgtgacgg
     9181 caaagtgacc gccagtgtgc aggtgacgaa caccggtaag cgcgaagggg cgacggtagt
     9241 gcagatgtac ttgcaggatg tgacggcttc catgagtcgt ccggtgaagc agctgaaagg
     9301 ctttgagaaa atcaccctga aaccaggcga aacccagacc gtcagcttcc cgattgatat
     9361 cgaggcgctg aagttctgga atcaacagat gaaatatgac gccgagcctg gcaagttcaa
     9421 tgtctttatc ggcactgatt ccgcacgcgt taagaaaggc gagtttgagt tgctgtaatg
     9481 ctttagtaag ggcgcgacgt ctgcgccctt taagtattta tctgattctg caactgaatc
     9541 cgcccagaca ggctaaccct gtcattatca actatgcttt tctcttaatt cgctgaaaac
     9601 aacagtaaaa gaatgaggaa agcatcatgc cactctcaaa gctctgggca ggttcattgg
     9661 ttttgttggc agccgtgagc ctgccgctac aggcggcttc ccccgttaag gtcggttcaa
     9721 aaatcgatac cgaaggcgcg ctgctcggca atatcatttt gcaggtgctg gaaagtcacg
     9781 gtgttcctac ggtaaataaa gtgcaactgg gaacgactcc cgtggtgcgc ggggcgatta
     9841 cttccggtga actggatatc tatccggaat ataccggcaa tggcgcgttt ttctttaaag
     9901 atgaaaacga tgcggcatgg aaaaatgccg ggcaaggcta cgagaaagtc aaaaaactcg
     9961 atgcagagca aaacaagtta atctggctga cgcccgcgcc tgcaaataac acctggacta
    10021 tcgccgtgcg tcaggatgtg gcagagaaaa acaaactcac ttcgcttgcc gacctgagtc
    10081 gttatctgaa agagggcggc accttcaaac tggcggcctc ggcagaattt attgaacgcg
    10141 ccgatgcctt acccgctttt gaaaaagcct acgactttaa actcgatcag gatcagttgc
    10201 tgtcactggc tggtggcgac acggcggtaa cgatcaaagc cgctgcccag caaacttctg
    10261 gcgttaatgc cgcaatggct tacggcactg acggtccggt cgcagcgctg gggttgcaaa
    10321 ccttaagcga tccgcaaggc gtgcaaccta tctacgcgcc tgcaccagtg gttcgtgaat
    10381 cggtgttgaa ggagtatccg caaatggcac agtggctaca gccagtcttc gccagcctcg
    10441 atgcaaaaac attgcagcaa ctgaatgcca gcattgcagt tgaaggactg gatgccaaaa
    10501 aagtggctgc cgactacctg aaacaaaaag ggtggacgaa gtaatttccc gtgacttatc
    10561 tacgtattaa tcctgttctg gcgctgctgc tgttgctgac ggcaatcgca gcggcgctgc
    10621 cgtttatcag ttacgcgcct aatcgtttag tttcgggtga ggggcgtcat ctctggcagc
    10681 tgtggccgca aacgatctgg atgctggtgg gcgttggttg cgcctggctg accgcctgtg
    10741 ttattcccgg taaaaaaggc agcatttgtg cactcattct ggcgcaattc gtcttcgtat
    10801 tgctggtgtg gggagctgga aaggcggcga cacaactggc acaaaatggc agtgcgctgg
    10861 cgcgtaccag cctcggcagt ggtttctggc tggctgcggc gctggcattg ctggcctgta
    10921 gcgatgccat ccgccgaatc tccacgcatc tgctgtggcg ctggttgttg catatgcaga
    10981 ttgccattat tccgctgtgg ttgctgtact ccggcacgct taacgatctc tcgctaatga
    11041 aagaatacgc caaccgtcag gatgtgtttg acgacgcgct ggcacaacat ctgacgttgc
    11101 tgtttggtgc ggtgctgcct gcgttagtga ttggtgtgcc gttgggcgtc tggtgctact
    11161 tttccactgc gcggcaggga gcgatttttt cgctactcaa tgtcattcag accgtgcctt
    11221 cggtggcgct ctttggcctg ttgattgcgc cgcttgccgc gctggttacg gcctttccgt
    11281 ggctgggtaa gctcggcata tcaggaaccg gaatgacacc cgcactgatt gcgctggtgc
    11341 tctatgcctt gctgccgctg gtgcgcggcg tggtagtcgg cttgaaccag atcccgcgcg
    11401 atgtgctgga gagcgccaga gcgatgggca tgagcggggc gcagcgattc ctgcatgttc
    11461 agttaccgct ggcgttaccg gtatttctgc gtagcctgcg ggtggtgatg gtgcaaaccg
    11521 tcggcatggc ggtgattgcg gcgttaatcg gcgcaggcgg ttttggtgcg ctggttttcc
    11581 aggggctgct aagcagtgcc attgatttag tgttgctggg ggtgatcccg gtaattgttc
    11641 tggcggtgct gatcgatgcg ctgttcgatt tgcttatcgc actgctgaag gtgaaacgta
    11701 atgattgaat ttagccatgt cagcaaactg ttcggcgcac aaaaagccgt taacgatctc
    11761 aatctcaatt ttcaggaggg gagtttttcg gtgctgattg gtacatcagg ctccggtaaa
    11821 tccaccaccc tgaaaatgat taaccgcctg gtggagcatg acagcggcgt gatccgcttt
    11881 gccggagaag aaattcgctc gctgccagtg ctggagttgc gccgccggat gggctatgcc
    11941 attcaatcta ttggcctgtt tccccactgg agcgtggcgc aaaatattgc caccgtgccg
    12001 caattacaaa aatggtcacg ggcgcggatc gacgatcgta tcgacgaatt aatggcgcta
    12061 ctggggctgg agccaaattt gcgtgagcgt tatccgcatc agctttccgg tggtcagcag
    12121 caacgtgtgg gagtggcgcg cgcactggct gccgatccgc aagtcttact gatggatgaa
    12181 ccttttggcg cgctggaccc ggtaacgcgc ggcgcgttgc aacaagagat gacgcgcata
    12241 caccgtttgc tggggcgtac cattgtgctg gtcactcatg atattgatga ggcgctacga
    12301 ctggcagaac atctggtgtt gatggatcac ggtgaagtgg tgcagcaggg caatccgctg
    12361 acgatgctga ctcgtccggc gaatgatttt gtccgccagt tttttgggca tagtgaactg
    12421 ggtgtccgcc tgctttcgtt acgtagcgtg gcggattacg tgcgtcgcga agaacgggca
    12481 gaaggtgagg cgctggcaga agagatgacg ctacgcgatg cgctctccct gtttgtcgcg
    12541 cggggatgtg aagtgctgcc ggtggtgaac acgcagggcg agccttgcgg cacgctgcat
    12601 tttcaggatc tgctggtgga ggcgtaagcg tatgaagatg ttgcgcgatc cgctgttctg
    12661 gctcattgct ttgtttgtgg cactgatttt ctggctgcct tacagccagc cgctgtttgc
    12721 tgccttgttc ccacaactgc cacgccccgt ttatcagcaa gaaagttttg ctgctctggc
    12781 actggctcat ttctggctgg tgggaatttc gagtttgttt gcggtgatca ttggcactgg
    12841 tgcaggaatt attgttactc gcccgtgggg cgcggaattt cgcccactgg tggaaactat
    12901 tgccgccgtt ggacagactt ttccgcccgt cgcggtgctg gcgattgccg ttccggtgat
    12961 tgggtttggt ctgcaacccg cgattatcgc cttgatcctt tatggtgtgc tgcccgtcct
    13021 gcaggcgaca cttgccgggc tgggagcgat tgatgccagc gtgacagaag ttgcgaaaag
    13081 tatgggaatg agtcgaggtc agcgactgcg taaggtcgag ctaccgctgg cggctccggt
    13141 gattctggcg ggtgtgcgaa cttcggtgat tatcaacatt ggtacggcga cgatcgcctc
    13201 aacggtaggg gccagcacgc tgggtacgcc catcatcatc gggcttagcg gatttaatac
    13261 cgcgtatgtg atccaggggg cgttactggt ggcactggcg gcgatcattg cagaccgcct
    13321 gtttgaaagg ctggtgcagg cgcttagcca gcacgcaaaa taaaggtata acctgcgagc
    13381 atgacgccac caattccgcc taacgccata aacaggaaca gggcgatgac cccaatttta
    13441 gctatgcgca taatgcactc cttatgttaa cgaaaggatt gtacagtaaa gcgcatttgt
    13501 taacgaatca ttaaatgccg agtgggaaaa tatcatggcc ttgttcctgc caactggtga
    13561 gttgctgctg ttgggcggag gttcgatttt caccgcacca caccagcaat gtacggcctt
    13621 cgaacagttc agggcgtagt tgattgagcg agtgggcgag gacatcaatg cgccatcctt
    13681 gttgactggc aatccagccc tccagccaca gacgggtggt atcgtgaata ttccagccaa
    13741 ccaccagcgc atctttaccc tgttttttac gtgccgaagc caggcaaatg gcgatgtagt
    13801 tgatcagtac gccgtcgagg atcgccagca gcgcctggag agtcggttgt tggcactgaa
    13861 gtcgtcggcg cagaggaata aacagatgcg tggtgagtgt ctgggcgggg taatcctgac
    13921 cgcgctcttt gatccacgtt cgcaggctat gcagattgcc gctttgcagg taagtcagta
    13981 atgtttcttg ctgatcgcgc cagccgttct gcacatcaac attttcatta ctgagcagca
    14041 ttttaacttt gctgacctgc acgccgttgt cgatccagcg tttgatctcg cggatccggt
    14101 caatatcggc atcattgaac agccgatgac cgccgtctgt ccgttgcggt ttcagcaatc
    14161 cgtaacgcct ctgccacgcg cgtaacgtga cagggttaat atcacaaagc aacgccactt
    14221 caccaattgt gtaaagcgcc atcgtctcac ccttgctcgc gaggtcccgg tttaacttta
    14281 gacgcagttt tgcgaaccag gtagttttgc ccgttttttg tgcatctata gggtgatttt
    14341 atttttgcca ggcgattttg agtgatcgta ctcacgaatt ctcatttttc tgcaagagtt
    14401 caaagaaagt taaacgcagg caatgtatgt tacgcgtttt aaagggaagt gtggtttgcg
    14461 ggtatgtacg attttaatct ggtgttgctg ctgcttcagc agatgtgcgt ttttttagtc
    14521 attgcgtggt taatgagtaa aacgccatta ttcataccgt taatgcaggt cacggttcgt
    14581 ctgccgcata aatttctctg ctacatcgtc ttttccatct tctgcatcat gggcacctgg
    14641 tttgggctgc atattgacga ttctattgcc aatacccgtg cgataggcgc ggtaatgggc
    14701 ggcttactcg gcggtccggt cgtcggtggg ctggttggtc tgaccggcgg gttacatcga
    14761 tattcgatgg ggggcatgac cgcgctaagt tgcatgatct cgaccatcgt tgaagggttg
    14821 ctcggcggcc tggtacacag catcctgatc cgtcgcgggc gcactgataa agtctttaac
    14881 cccattaccg ccggtgccgt cacgttcgtc gctgaaatgg tgcaaatgtt gatcatcctg
    14941 gcgatcgccc gaccttatga agatgcggtg cgtctggtga gtaatattgc tgcgccaatg
    15001 atggttacca ataccgtcgg cgcggcgctg tttatgcgta tattgctcga taaacgcgcg
    15061 atgtttgaaa aatacacttc ggctttttct gccactgcgc taaaagtggc tgcctcgacg
    15121 gaaggcattt tgcgccaggg gtttaacgaa gtgaacagca tgaaagtggc acaggtgctg
    15181 tatcaggagc tggatattgg tgcagtcgcg attaccgatc gagagaaatt gctggccttt
    15241 accggaattg gtgacgacca ccatttaccc ggcaaaccga tttcttcgac ttacacctta
    15301 aaagcgattg aaaccggtga agtggtctac gctgatggca acgaagtacc ttatcgttgc
    15361 tctttgcatc cgcaatgcaa actggggtcg acgctggtca ttccgttgcg tggcgaaaat
    15421 cagcgggtga tgggcaccat caaattgtat gaagccaaaa accgtttatt cagttcaatc
    15481 aaccgcacgt tgggcgaggg gattgcgcaa ctgctttcgg cgcagatcct cgccgggcaa
    15541 tatgagcggc aaaaagcgat gctcacccag tcagagatca aactgcttca cgcccaggtg
    15601 aacccccatt ttttgtttaa tgcgcttaac accattaaag cggtgatccg ccgcgacagc
    15661 gaacaggcca gccagctggt ccagtatctt tccacttttt tccgcaaaaa cttaaagcgg
    15721 ccttcggagt ttgttactct cgcagacgaa attgaacatg tgaacgctta tctgcaaatt
    15781 gaaaaggcgc gcttccagtc gcggttgcag gtcaacattg ctattccgca agaattatcc
    15841 cagcagcaat tgcccgcgtt taccctgcaa ccgatagtgg aaaacgccat taaacatggg
    15901 acatcacaac tgctggatac agggcgagtg gcgatcagcg cccgacgtga ggggcaacat
    15961 ttgatgctgg agatcgaaga caatgccggt ttgtatcaac cggtaaccaa tgccagtggg
    16021 ctggggatga atctggtgga taagcgttta cgtgaacggt ttggcgatga ctatgggata
    16081 agcgtcgcct gtgagcctga ttgttacacc cgaataacgt tacgactacc atggagggac
    16141 gaggcatgat taaagtctta attgtcgatg atgaaccgct tgcacgggag aacctgcgcg
    16201 tatttttgca ggagcagagc gatattgaaa tcgttggaga gtgttcaaac gccgtagaag
    16261 ggatcggcgc ggtgcataaa ctgcgcccgg atgtgctgtt tctcgatatc cagatgccgc
    16321 gcatcagtgg tctggaaatg gtggggatgc tcgaccccga acatcgaccg tatatcgttt
    16381 ttctcaccgc gtttgacgaa tacgctatta aagcctttga agaacatgcc tttgactatc
    16441 tgctgaagcc aattgatgaa gcgcgactgg agaaaacgct ggcgcgtttg cgtcaggaac
    16501 gcagtaagca ggatgtttcg ctgttaccgg aaaatcaaca ggcgctgaaa tttatccctt
    16561 gtacggggca tagtcggatt tatttactgc aaatgaaaga tgtggcattt gtcagcagtc
    16621 ggatgagcgg tgtctacgtt accagccacg aagggaaaga gggctttacc gaattgacat
    16681 tacgtaccct ggaaagtcgt acaccactac tgcgctgcca tcgtcagtat ctggttaacc
    16741 tcgcgcattt acaggagatt cgtctggaag ataacggcca ggccgagttg attttgcgta
    16801 atggcttaac cgtgccggtc agccgccgtt atctgaaaag cttaaaagag gcgattggcc
    16861 tgtaaaagac tgctaaaatg gctttttgcc tcatcaacac ctgaaggcct catgctaagt
    16921 aacgatattc tgcgcagcgt gcgctacatt ttgaaagcca ataataatga cctggtgcgt
    16981 attctggcgc tgggtaatgt cgaagccacc gcggaacaga tcgccgtctg gctacgtaaa
    17041 gaagacgaag agggttttca gcgttgtccg gacattgttt tgtcgtcatt cctcaatggc
    17101 ctgatttatg aaaaacgcgg caaggatgag tctgctccgg cactggagcc ggaacgtcgc
    17161 attaataaca acatcgtgct gaaaaaatta cgcatcgcgt tttcgctgaa aaccgatgac
    17221 attctggcga tcctcaccga acagcagttc cgcgtttcga tgccggaaat tacagcgatg
    17281 atgcgcgcac cggatcataa aaacttccgc gaatgcggcg atcaattttt acgttatttt
    17341 ctgcgtggac tggcagcgcg ccagcatgtg aagaaaagct aagacgggta tggcggccat
    17401 gcaaaaacat ggccgccgac agattatttc acttctttaa aaccagcggc tttcatcacc
    17461 agttccattt gcgccatagt gatacctttt ttggcatctt cagcagaaac gttgattcct
    17521 gaaataccct gcagggcttt gaaatccact ttttccatat cgatagtcac gttttcctgc
    17581 gcgtaagtat cggtataggt taatttttct tcaacacccg cgatgttttt gtatttggcg
    17641 cttaacggct caagtgtcct ggcagcgtct tctttggtgg ttgcaccaat ggaggcaaat
    17701 tgaattttgg tttcagaaga ttgcttaagc accttgtcac ctttgtagac ataggtaatg
    17761 gcaatttcag taccgttcag attggcgctg aattttttcg attcttcttt gtcaccgcag
    17821 ccagcaagag agaaaaccag aacagatgca acaacgaggg aaaacagctt attgaaagcc
    17881 ttcatgtaaa actccatttt atttaatcaa ggaactggtg actctcacca ggggctatat
    17941 aggatatgcc taataccgtg gcgtgagcag tccggaactg gagtagaact cttagtaaaa
    18001 agcactattt catccttgtt gctgaagcat ggggaataat tgttcgcaca gcaaaacacc
    18061 gttattcatt gcttctaccc gtgcctcgct ttctgtatta cgaaattgtc ccaacacatg
    18121 tgccagccga taaaaaccca ccgcggtgag gtcattcgcc agcaactctg cctgaccaat
    18181 agcactatgt tcctgatagc gccagccgtt atggagcagt tgaataagta acgcctggca
    18241 gcgcatcagc aactgatgag cagtagacgg cacaggcaga acgctggcag aaggtagtga
    18301 tgccaccaca ggcgcagttt ctgcgtccag cgcccaggca cgagtctttg tcatcattac
    18361 ctgtggttcc agtgtcaatt gcccctctac aaagctgaca aagccagaaa ccagacacac
    18421 ggggtcgtct gtttgttgca aaagcgccgc catgcgttca acggcataag gcgcgctggc
    18481 tgaggctggc agtgataacg tcagcagatt atcttcacct tcgccgctga ttacctgcgc
    18541 atccagcgtc tggcggctgc tatcccaacc gagcgaaata cactcagcga ccggcagaat
    18601 aaataagtta tcgacctgat taagaggccg tatgcaggcg gggggacgct ggcgtaaata
    18661 ttcccgcaaa gccacaatgc ccggctggcg taacggcgcg ctcaacattt gccaggcatc
    18721 aggcgacagc ggcacaacgc tgcttaagcg gttgcgggta gctaacagca gctcgccatc
    18781 ggcactgcgt tttgctgctt gtgaaacaat ttgcccgccc gccagtgcgc cagcctgaaa
    18841 actaaacagc cgacgcgtcg ctgccggtga gttttcctgt tcgcttctcg gccaactacg
    18901 cgaaagatgc aaaatactgc cggtgtcggg atcggtaaac cagatgcgta aaccataatg
    18961 ctcaatatcc tgccagcaac gcatacctaa agacaccagc cgcagatgat caagctttgc
    19021 ttctccggca atgccagagc caacgaccgt gcgccacggc atgggcggta cttcaccgac
    19081 actgtcacgt cgcgctatct cttgtgcact gttcaagcga ctggttaacg ccgcaagctg
    19141 gcacaagcat tttccggcat gatagtggct ggcgcgggcg tggaaggcat caacgctggc
    19201 gcgcagttgc cgtagcgatt cactcaccca tcgccagttg cagcgttccg ccgcctgctg
    19261 tgcgcggctg aaagcggcct cgtaatggat tggcggctgg ctgatgccgc ccagccataa
    19321 tgcctgactt aattgctgaa catattgacg acacgcgttg ccctcttcgc tggcaaacgg
    19381 atcgtcagat gatgtgacgt gttcgctgcg catctgccag attaaatgag taaattccgc
    19441 ttgctgagtt ttggcctcga cgaaggcctg cacagccagt acgacatgtt cgcaaagtgt
    19501 gccgtcaata caatcacagc gagcgaaacg aatgctgctg cgggaataaa aacgtacatc
    19561 gctcatcggt aagcgggcag agggaatttc gcccggcgta cagaacaact caatggtgat
    19621 gcctttagcg accagcgcct gtgcgcgttt gcgggtggca tcgggcaggg tagccagttc
    19681 ttccagccag attgccggat cccactcttc ttctttttcc gtaggctggg tggtggcgca
    19741 aagtcgctga taacttaaaa ccagcatcac gcgatggcgg cacatgccgc tggccccgca
    19801 gctgcactga gcctctttca gtgcctggcc gttcgccagc tgggtgcgga caccgtcact
    19861 gaaggtggcg attaaagcgc cgttctcatg actgatttcc gggacgttgc cattttccag
    19921 ttctttaagg ctgcgcttaa caaaaccggc attgcttaac gccgtcagtg cctgtggtgt
    19981 cagttctaat aattccggac gtagtgaatt catgactgaa gattctccgc aagccaggtc
    20041 gccagttcgc ccggcgtcat ggcggctatt tgtgcaccga cattaaccag tgcctgggcc
    20101 atatcgcggt catagcaagg tgttgcggtg ctatcgagcg ccgccagtcc cagcactttg
    20161 atgccgctct ggacacactt tttcacctga tgcgtcagca atgatgatga accaccttca
    20221 tagaaatcgc tcacgaggat aatgacactt tttgcaggtt gttcaataag ttgccgacca
    20281 tactccacgg cactggcgat attggtcccg ccgcccagtt gtactttcat taataactct
    20341 accggatcgg caacgtctgc cgtgagatca accacgctgg tgtcaaacgc caccagatgg
    20401 gtacgaatgc cgggtaactg ccataaacag gccgccatca ccgctgagtg gatcaccgag
    20461 tcaaccatcg atccgctttg atcaaccagt aagaccagtt gccattgatc gctatggcgc
    20521 ttaatgcggc tgttaaagcg gggggattcg atatacaact tgccgtgttg cggatgccag
    20581 tgttgcaggt tggcacgcag cgtacttttg aaatcgaaat tacgcgccag tggaataaat
    20641 gagcgacggc ggcgatcgcg gacaccagaa aaagcctgac gaacttcctt tgccagtcgc
    20701 gccataattt cttcaacaac ctggcgcact attcggcggg cggcagccag cacttcggga
    20761 ttcatcagat gtttggtgtg caaaacggcg cgtagcaggc tttcagaagg ctgcatacgt
    20821 tccagcacgt cgagattcgt caccacatct tcaatgccgt agcgcagtac ggcatcgctt
    20881 tccagtcgct cgatcacccg ttgcggaaac agcgtgtgaa tactgttgat ccactcaggg
    20941 gtggtgagat ttgagtcacc taatccaccg gagcgttcac cacgctggag ccgttcagga
    21001 tcgcgcccat acagccactc cagcgcgtgg tctatctgcc gggcgttgtc atccagccca
    21061 caaagcgtcg tttctgccgc ttcgccaaga attaatcgcc agcgttgtag ctcacgggtg
    21121 gtcagaagat cgttcagttc agacatggaa aactccccag tgttgcagcg atgccagtgc
    21181 ctgctgttcg agttgttgat gatgtgcaat gtcttgtggt ggacaatgca acggcatttg
    21241 cagtgccgaa acgggaagtt gcaccagttg ataatgctca agcacctgat gcgccagcgt
    21301 cccgcgttct cgtggtggta gccaggccat cgccgcgcgt aaatcgggca gggcattgat
    21361 aaaatcggca tcactcagtt gatttaaatg actgctgaaa ccggcgataa ataccggctg
    21421 acaggccagt tgatgacggg ctaatgccag caaaccgtgc agcgcctcgc cggattgtgt
    21481 cggggataat tgcaccagca ttgtcagcgc cgcttcggca ctggcgttgg gatgttccag
    21541 acgcatcagt gcgccaagtg ccgcgccgcg atccagcgcc ggggcatgaa tcgcctgact
    21601 gcgtcgctcc agtaaggcta ccgccgcaga aagagaaacg ccgggtaaat taacgccgct
    21661 atgtagatcg cgcaggatat ggcaaagcgc ttgccagcta tgcaggtgag cgtgaaactc
    21721 cttttcatcc ggtctgccgt tggattcaca tagccacagc gtgcgatcga tggccgcgca
    21781 taatgtcgtt tgtaatatct gcgcgccctg cataccgcta atttcatcca gccgccataa
    21841 ggcatatagc acttccagcg cctggcccat ttcggcaaac tgattttcct gggcgattaa
    21901 taatgtgagt tgctccagta attgttgatt gaaggacgcc agacccgcta acgccgcctg
    21961 gctaagacat gtggtgatac tgccgattcc acccgcgtcc agcatatcgg cttctaattt
    22021 attgcgtgcg gcttcctgga gtgtggcacc aaagcaggcg gcctcaatta atgccgcatg
    22081 ttggctaagc gggcgggtta atttccagcg ttcttcaccg ttgcctgcaa gtgtcagtgt
    22141 acttccctgc tggcgcacaa cccctgggat ctccagtatt accagccgat gtaacacctg
    22201 actttgcgcc agtccatcgg gggtaaagcg atttaaagta agctccgccg gaaaagagat
    22261 tcctgtacgt tccagttcgc aggtgacatc ttttggcaga ggcggctgtg gcgtagaggg
    22321 ggcaagttta ccgaatccgt cacccgctaa tgtgtctatc aacgttagca gaatcggatc
    22381 ggtatcggga tgaataacgc cgcgatagct ccacggcaac ggcgcattca gggcttcttt
    22441 aatcagcgag cctgctatgg catccagcca gtcagtgcgt aacggtagtg tatgaccacg
    22501 caactgtgcc agtgccatcg catgcagatg agcggcagcc atatccgctg ttgaagcagg
    22561 caagtggtgc tggcgcaaac gggtaagaac cgttttcagc agttgttcac ctgcctgctg
    22621 caagccccac cgccagcacc agttttgcca gaccggggca ggcataccgg aaaggtatcc
    22681 tgccagcaca tcaaggcgct tttcactgta gggggtgaga taacaacctg taacggcatc
    22741 tgccagcgag ggcaattccg gcgtgttaat ttcctgcggg cattcgcgcc acatttttgc
    22801 cagtgcagaa gcgtgccagc caccgcagac gactaacacg tcgccattgt tttgctgcat
    22861 cgcccaacca atccagcggg ccataaagtc ttcgcgctga cgattgagag catctccggg
    22921 ggaatcgccc cgcagttgtg caaaatagtg cgccagtgca gagggtaatg cagtttgctg
    22981 gctttcatct tcgaacaagt gatcccacag ggtgtcgctg ttatccatgc gggtggcacg
    23041 cagcagcaag gtctggcttt cctcttgcgt atcaggcgaa tcgtcctctt cttcactctg
    23101 cgcccagcaa ggcaaatcga tgaagtaagt ttgtgcctga atacgacgag cggcttgtag
    23161 cgcctgccac tccggcgaaa attcagcata tggcgtccag gcaccacgcc cgggggctgc
    23221 accgtcctga tactggcaat aactgtaaat agctaccgga agctggtggg ctaaaaacag
    23281 ttcgtctacc cggtcattaa aatcagccgg gccttcaatc aacacgtatc gtggccgctg
    23341 gctttcgatt aacgatttca ccagccgggc gcaggccgga ctatgatgcc ggatgccgac
    23401 aattaacggc tcgctcatgc atctttcctc acggcaggcg gtggcgagct tgataataag
    23461 cctgccagtg agcttcttta tgtgtggcga ctcgctgttc gaagtaacgg cgcagacgag
    23521 cgcgatcttc ttcgttatct ttgacgatgg ttccggcaat acagtccacc agatcggctg
    23581 gctcacctgc gcgattcgct aaaaaccagg cgcggacgcc cactgcgtgg gccacgttca
    23641 cggcttcagc ggtggacata attgccgtca gggtatccat tgacgttttc ttttcgccgt
    23701 tggcacgcag atcgcggaag gtgcggacca gtaactccag taccgcgtca ggcactttat
    23761 gcggaatacc gctatgggcc agcaaacgcg ccgaagcact ggcgaccagt tccagctcct
    23821 gggcgaaatc cataatcgga aagacggttt caaaatcgaa gcgacgcttt agcgcggcac
    23881 tcatctcatt gacgccgcga tcgcgggtat tggcagtggc aataatatta aagccttctc
    23941 gcgcatagag ctgactggct tcaccggtga gttccggcac cgtcattacc ctgtcagaaa
    24001 gcatacctaa cagacagtct tgtacttcca gcggtgtacg cgttatctct tcaaaacgga
    24061 caattttgcc gtcgcgcatt ccctggtaaa gcggggcagg gaccagcgct tccgttgacg
    24121 gaccatggtt gataagcagg gcgtagttcc agccgtactt gatttgatct tcggtggtag
    24181 atgcgccgcc ctggatggtt aacccggcat cgccgctaat agcggttgcc agtaattcag
    24241 acaggagcga ttttgcggtg ccaggttccc ccaccaacat caaaccgcgc ccggtagcga
    24301 gagtgactaa catgcgttcg acagcggagg gactgatcac gactttacgg ctaatatttt
    24361 gcgcgctatc gccaaggata aaagcgcggg ccgcaggcaa acttaactgc caaccgggcg
    24421 ggcaaggtgc gtgatcattt tgttttaatt ttgccagttc atcggcgtat aacacagcgg
    24481 caggcggacg ctgcagatga ttattctgcg gtgacatata ttcatccctg aaatgtatta
    24541 atgaaataaa cgatatttta cgcgcaataa atgtttgccg cattagttgt ttgagcgaaa
    24601 aatatctgga aagcagatca cagtaaataa aaaaacctgc tggtgaggca ggttctttta
    24661 tttactacaa tgacgctaac ttgctgattg tgcaacattt cgtaccaacg aataaattat
    24721 aaccatatcc acactccatt gcaattcagt gaatgagatt tataatcctg cccgttggga
    24781 ggttgtgccg gatcggacca attttaagtc ctggctgctg gacgggtggt gccgcaggcg
    24841 gtgccctgat ccggagagca acgtttcgta ccaacatacg taaaccagta tcgacaggca
    24901 gtgtaagggt aaggtgctgg tgttgcgctc cttaataagg agagcggatg gacacgctga
    24961 cacaaaagtt aaccgtgctc attgctgtac tggagctatt agtggctctg ttacggttga
    25021 ttgatttgtt gaagtaacgg gcaaattgaa acgcatctaa gggggaatac cgtttccccc
    25081 tttaataaaa tgattactgt ttttattaat tcatcatata actcattttt atttataaat
    25141 tcatcgattt tataatcaac gtactatttg gccgggatgt gaatatttat ggtgggctat
    25201 tggcgaaaaa tatctggaaa gtagatcgca gaaaataaaa gaacctgctg gtgaggcagg
    25261 ttcttttatt tactactttg atgttaagtt actgatagtg caacatggca tctcaacgaa
    25321 cgaattataa ccaccgctgg accgtgttgc aattaagata atgagattta taatcctgac
    25381 tgttgggaag ttgcgccgga tcgaacccat ttcaagtcct ggctaccgga cgggtggtgc
    25441 cgcaggtagt gctctgatcc ggagtgcaac atggcgactc aacaaacgta aaccagtgtc
    25501 gacaggcagt gtaagggtaa ggtgctggtg ttgcgctcct taataaggag agcggatgga
    25561 cacgctgaca caaaagttaa ccgtgctcat tgctgtactg gagctattag tggctctgtt
    25621 acggttgatt gatttgttga agtaatgggc aaattgaaac gcaactaagg gggaaaaccg
    25681 cttccccctt taataaagta atattctatt agttggttga atatattctg tagtttacga
    25741 ttaaaaattg ctattacagt ttcaggtgat tcacctcgta atttccgctc atcaatacac
    25801 tcatctctta tataaatcaa cgcatcattt attagcgggt cagcatcttc tcgatcacgt
    25861 tgatgaaatg ttggacgtag ccggaatcgc tccagtcgat agacggaacc tgtaaataat
    25921 tcagagcatt tatggatctc aagtctgaag gaaaacggat cttcaggtac agggacctca
    25981 taatcgtaaa tagcaaccag ctctttttgc acgatcatcg ctttccccgc tggtaagtcg
    26041 tgttgataac gggcagttgc atcactgccc gctgtgtttt taatggaaaa tgacgtcagg
    26101 tgcgcttaac gcctcactca aatcaaacac atcaagttcg gcaaaagtga cggatttatt
    26161 gtcacggtac actcgaagag aacggaagct cattaacgct ttgctatcaa cattgtagtg
    26221 gttaaaaccg ggtgaaaact taaggaccag ccggtactcg cccaggccat agacaaatgc
    26281 gtcctcatag cctgactccc agcctttgta tcgcattccc ataagct
//
LOCUS       DAZGHF010000067        26081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGHF010000067 DAZGHF010000000
VERSION     DAZGHF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.067"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            126..241
                     /gene="rrf"
                     /locus_tag="ACHK3A_002821"
     rRNA            126..241
                     /gene="rrf"
                     /locus_tag="ACHK3A_002821"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            567..1865
                     /gene="kgtP"
                     /locus_tag="ACHK3A_002822"
     CDS             567..1865
                     /gene="kgtP"
                     /locus_tag="ACHK3A_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO8740878.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1862..2185)
                     /gene="yfiM"
                     /locus_tag="ACHK3A_002823"
     CDS             complement(1862..2185)
                     /gene="yfiM"
                     /locus_tag="ACHK3A_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO8740879.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(2229..3584)
                     /gene="pssA"
                     /locus_tag="ACHK3A_002824"
     CDS             complement(2229..3584)
                     /gene="pssA"
                     /locus_tag="ACHK3A_002824"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO8740880.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3698..6358)
                     /gene="pat"
                     /locus_tag="ACHK3A_002825"
     CDS             complement(3698..6358)
                     /gene="pat"
                     /locus_tag="ACHK3A_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO8740881.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKSNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6390..7088)
                     /gene="tapT"
                     /locus_tag="ACHK3A_002826"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6390..7088)
                     /gene="tapT"
                     /locus_tag="ACHK3A_002826"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO8740882.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPETLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTEAATGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7157..7576)
                     /gene="trxC"
                     /locus_tag="ACHK3A_002827"
     CDS             complement(7157..7576)
                     /gene="trxC"
                     /locus_tag="ACHK3A_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO8740883.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7783..8820
                     /gene="yfiF"
                     /locus_tag="ACHK3A_002828"
     CDS             7783..8820
                     /gene="yfiF"
                     /locus_tag="ACHK3A_002828"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO8740884.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVLQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8868..9557)
                     /gene="ung"
                     /locus_tag="ACHK3A_002829"
     CDS             complement(8868..9557)
                     /gene="ung"
                     /locus_tag="ACHK3A_002829"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO8740885.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREDVV
                     FLLWGSHAQKKGAIIDKQRHHILKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            9862..10245
                     /gene="grcA"
                     /locus_tag="ACHK3A_002830"
     CDS             9862..10245
                     /gene="grcA"
                     /locus_tag="ACHK3A_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO8740886.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10301..10888)
                     /gene="eamB"
                     /locus_tag="ACHK3A_002831"
     CDS             complement(10301..10888)
                     /gene="eamB"
                     /locus_tag="ACHK3A_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO8740887.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWIIGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10991..11872
                     /gene="yfiE"
                     /locus_tag="ACHK3A_002832"
     CDS             10991..11872
                     /gene="yfiE"
                     /locus_tag="ACHK3A_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8740888.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            complement(11905..13239)
                     /gene="srmB"
                     /locus_tag="ACHK3A_002833"
     CDS             complement(11905..13239)
                     /gene="srmB"
                     /locus_tag="ACHK3A_002833"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO8740889.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13371..14108
                     /gene="trmN"
                     /locus_tag="ACHK3A_002834"
     CDS             13371..14108
                     /gene="trmN"
                     /locus_tag="ACHK3A_002834"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO8740890.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14093..15715)
                     /gene="nadB"
                     /locus_tag="ACHK3A_002835"
     CDS             complement(14093..15715)
                     /gene="nadB"
                     /locus_tag="ACHK3A_002835"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO8740891.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDDRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRCMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15971..16126
                     /gene="rseD"
                     /locus_tag="ACHK3A_002836"
     CDS             15971..16126
                     /gene="rseD"
                     /locus_tag="ACHK3A_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO8740892.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            16123..16698
                     /gene="rpoE"
                     /locus_tag="ACHK3A_002837"
     CDS             16123..16698
                     /gene="rpoE"
                     /locus_tag="ACHK3A_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO8740893.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16731..17381
                     /gene="rseA"
                     /locus_tag="ACHK3A_002838"
     CDS             16731..17381
                     /gene="rseA"
                     /locus_tag="ACHK3A_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO8740894.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17381..18337
                     /gene="rseB"
                     /locus_tag="ACHK3A_002839"
     CDS             17381..18337
                     /gene="rseB"
                     /locus_tag="ACHK3A_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO8740895.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18334..18813
                     /gene="rseC"
                     /locus_tag="ACHK3A_002840"
     CDS             18334..18813
                     /gene="rseC"
                     /locus_tag="ACHK3A_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO8740896.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            19011..20810
                     /gene="lepA"
                     /locus_tag="ACHK3A_002841"
     CDS             19011..20810
                     /gene="lepA"
                     /locus_tag="ACHK3A_002841"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO8740897.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20826..21800
                     /gene="lepB"
                     /locus_tag="ACHK3A_002842"
     CDS             20826..21800
                     /gene="lepB"
                     /locus_tag="ACHK3A_002842"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO8740898.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            22072..22752
                     /gene="rnc"
                     /locus_tag="ACHK3A_002843"
     CDS             22072..22752
                     /gene="rnc"
                     /locus_tag="ACHK3A_002843"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO8740899.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22749..23654
                     /gene="era"
                     /locus_tag="ACHK3A_002844"
     CDS             22749..23654
                     /gene="era"
                     /locus_tag="ACHK3A_002844"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO8740900.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23666..24394
                     /gene="recO"
                     /locus_tag="ACHK3A_002845"
     CDS             23666..24394
                     /gene="recO"
                     /locus_tag="ACHK3A_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO8740901.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGESVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24406..25137
                     /gene="pdxJ"
                     /locus_tag="ACHK3A_002846"
     CDS             24406..25137
                     /gene="pdxJ"
                     /locus_tag="ACHK3A_002846"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO8740902.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIPVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            25137..25517
                     /gene="acpS"
                     /locus_tag="ACHK3A_002847"
     CDS             25137..25517
                     /gene="acpS"
                     /locus_tag="ACHK3A_002847"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO8740903.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25939..26019
                     /gene="shoB"
                     /locus_tag="ACHK3A_002848"
     CDS             25939..26019
                     /gene="shoB"
                     /locus_tag="ACHK3A_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO8740904.1"
                     /translation="MTDCRYLIKRIIKIIIAVLQLILLFL"
BASE COUNT         6397 a         6489 c         6891 g         6304 t
ORIGIN      
        1 cggtactaat gaaccgtgag gcttaacctt acaacgccga aggtgttttg gcggatgaga
       61 gaaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg atgaaacaga
      121 atttgcctgg cggccgtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
      181 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
      241 catcaaatta agcagaaggc tatccgtcag gatggccttt ttgcgctgga gcagaataaa
      301 tgccggatgc gacgctggcg cgtcttatac tcccacataa gctagattca gcagcgaata
      361 cggcttcccc aacgtgccca cctccatacg tgtcctcctt accagaaatt tatccttaag
      421 ctcctcaata accattttcc tgctaactaa attcgtggtt aaggttgcat aatgatatgc
      481 aacaaatgta taacattttc tttaccaata gaaataaaca aaagcgaccg acaaaagcat
      541 cggattacgg caggagacat aatggcatgg ctgaaagtac tgtaacggca gacagcaaac
      601 tgacaagtag tgatactcgt cgccgcattt gggcaattgt gggggcctct tcaggtaatc
      661 tggtcgagtg gttcgatttc tatgtctact cgttctgttc actctacttc gcccacatct
      721 tcttcccttc cgggaacacg acgactcaac tactgcaaac agcaggtgtt tttgctgcgg
      781 gattcctgat gcgcccaata ggcggttggc tatttggccg catagccgat aaacatggtc
      841 gcaaaaaatc gatgctgtta tcggtgtgta tgatgtgttt cggatcgctg gttatcgcct
      901 gcctcccagg ttatgaaact ataggtacgt gggctccggc attattgctt ctcgctcgtt
      961 tatttcaggg attatccgtt ggcggagaat atggcaccag cgccacctat atgagtgaag
     1021 ttgccgttga agggcgcaaa ggtttttacg catcatttca gtatgtgacg ttgatcggcg
     1081 ggcaactgct agctctactg gttgtcgtgg ttttacaaca caccatggaa gacgctgcac
     1141 ttagagagtg gggatggcgt attcctttcg cgttaggagc tgtgttagct gttgtggcgt
     1201 tgtggttacg tcgtcagtta gatgaaactt cacaacaaga aacacgcgct ttaaaagaag
     1261 ctggatctct gaaaggatta tggcgcaatc gccgtgcatt catcatggtt ctcggtttta
     1321 ccgctgcggg ctccctttgt ttctatacct tcaccactta tatgcagaag tatctggtaa
     1381 atactgcggg aatgcatgcc aacgtggcga gtggcattat gactgccgca ttgtttgtat
     1441 tcatgcttat tcaaccactt attggcgcgc tgtcggataa gattggtcgt cgtacctcaa
     1501 tgttatgttt cggttctctg gcagccattt ttaccgttcc tattctctca gcattgcaga
     1561 acgtttcctc gccttatgcc gcttttggtc tggtgatgtg cgccctgctg atagtgagtt
     1621 tttatacttc aatcagtgga atactgaagg ctgagatgtt cccggcacag gttcgcgcat
     1681 taggcgttgg tctgtcatat gcggtcgcca atgctatatt tggtgggtcg gcggagtacg
     1741 tagcgttgtc gctgaaatca ataggaatgg aaacagcctt cttctggtat gtgaccttga
     1801 tggccgtggt ggcatttctg gtttcactaa cattgcaccg taaaggaaaa gggatgcggc
     1861 tttagtgacg ggtcagttgc cagacggtat agccggtgct tgcaccggcg atatcccagg
     1921 caaaatcctt ccagctccag ccgctcccct cagggcggct atcccaaagc tcttttgacg
     1981 cccccagact gacagagaac atcaatccaa acatggcact gcgatcccgg ctcatcccct
     2041 gatgctgtgc atattcattt ccggcggcgg aaagcatcgc cgaggcgata aagtgttgag
     2101 ctttatcctg cccgctccag ctatcgttag ccatatggct gcatccagaa agtaacaata
     2161 gcgaacagac aaaaagaata cgcaaaacgt ggcctccaaa acaaaccccg tacaggacgg
     2221 ggttgtgatt acaggatgcg gctaattaat cggtcgatgc ggatacggcg caaacgacgg
     2281 atgagtttac gaaccttcac cggataatcg gcaatacttt gcagatcgcg atagtgctta
     2341 acgatggtgg tatgctcgcg gatcagctcc agttctttct ctcgctgtgg cgcaagctcc
     2401 agttgcggat cgtggatcaa aatggcgttt tccagatcca gacgccaggc gcgcgggttc
     2461 aggttattac cggtgatcaa catccactta tcatcaaccc acatcccttt taggtgatag
     2521 gtgttgtcgt catctttcca taaccgaacc actagctggt cagtattgac gtaatactgc
     2581 aaacggctca ggaaacgacg cagattgatc tcatagagat aaggcaatgc gccaattatc
     2641 ttgaaaggtt catcttccgg aatgtagaag tcgttcgcgg ttttatcacc aacaataatt
     2701 tcgacctttt tcccttcgcg cagcaactgg ataatattgc gcacaaggat tgctggcagg
     2761 ttgaagtatg gcgtacagat ggttagtttc tgctcggcac aaggcataag atggaaaatg
     2821 gtcttgttca acagactcga tttccccagc cccactagcg gcgttacgga aagctgatcg
     2881 ttgtcggcat cgccctggaa atgataagcg gcatcacgca gctcctggcg gaacaggcga
     2941 atgtcgttct tgatttccgg gctttttggc cgattaacat catccagacg attaacgccg
     3001 cggccattca taatattctg tgtaacccat tcaaacataa tgtctgacat cttacggtta
     3061 cggattagat gataacggtc gtagcgatat ttatcgtgct gatgcaggta aacatcgttc
     3121 aggctggcac cgctataaag tacgctatcg tcgatgataa agcctttaaa gtgcagaaca
     3181 ccaagggctt cacgggtatt gattggaacg ccataaaccg gaacatctac gcccggattt
     3241 tcctgcgcca tgcggcagta ccagtcagcg ttagtgttag atgccgcagc gccaatgcgt
     3301 ccacgttgtg cacgatgcca gtcgaccagc acccgcacat ccagttccgg acgctgcctt
     3361 ttagcctcat acaacgcgtt cagaatgcct ttgccaccgt catcctgttc gagatacagg
     3421 gcaacaatgc aaatgcgctg cttcgcgctg gctatttttt ccagcaacgt ctcccggaag
     3481 tcggcgggag cgtaaaagaa atcgacatca tcaactgatt gagaaatctt gggtagttgg
     3541 gcaaggtgtt gttgatgttt attacgctta aattttgaca acatcacagt gcatttcttc
     3601 tctgttcatt gaagggtcct ctgtgcaatg cagacgacat aagcgggcaa taataacacc
     3661 agtcccgatt aagtggtcaa catttccagt accttactca tgattcctcg cgctgggcaa
     3721 gatttagcgt aagcccaacg atcccctctt cgagctggat atcaacgtta aacccgagct
     3781 tgcgggccag cgccaccatg ccacgattgt ttggcatcgt aataccattc agacgttgta
     3841 gtccgtgatc tcgcgtatag gtaatcaact tttccattaa gcgtcgacct aagcctaacc
     3901 ctttgagatc cgagcgaacc agcacggcaa attcggcatc gatgttatca gggtcggaga
     3961 tcgcacgcgt gacgccgagg atctcttctg tttgatcaat acgtcgtacc gccacaaaag
     4021 ccatttcccg atcgtagtcg atctgcgtca tgttggctaa atcttcatgg gtaaattcgt
     4081 tgatctcgct aaagtagcgg taataaagat cttctttggt gactcgcgaa atgaattgct
     4141 gaagctgtgg ctcatcttct ggcaaaatcg ggcggaacaa gcagcgttca ccgtttttca
     4201 attctaccca ttcttccagc tgatgcggat aagggcgcac tgccagccga ctctcgttat
     4261 cgccttcaaa cggcgcgata tccagcgtga catccagcgc ggtaaattca ctgccagaag
     4321 ccagcaaagg atgaatatcc agacgctgaa tttccgggca atcgacaatc aagttagaaa
     4381 cctgcaccag aagctggctc aagcctgcaa catctaatgg gcgtagcgca ctgcgcgcac
     4441 gaatcttttt acttttgatc ccctgaataa ccagatagcg ggccaggttc atgttcagcg
     4501 gcggtagtgc gacgacggct tgatcttcag gacgccactc cacaccgcct tcgcccagca
     4561 tgatcaacgg cccgaaaacc ggatcgtgct caaccacaac ccgcaactcc tgagcgccag
     4621 cacggttagc catactttgc accaacaggc cgtggatccg cgcctgcggc caggccattt
     4681 ttacgcgatc gaaaatagcg tttgccgctt gctggacttc attagctgta cgcaggtaga
     4741 gcattacgcc ctgaacttcc gatttatgtg gaatatccgg cgaacgcaat ttcagcgcca
     4801 ccggataacc aatctgttcg gcaatatgca ccgcttcggt gctatcgctg gcaatccagg
     4861 tggggagcgt gttcatgcca tacgcttgca ggatgggctg aacttcatgg gtatcgagcg
     4921 acgtagcccc ttcggcaatc gcctgttgca acagaagatg cgcttctgcg gtattagagg
     4981 tcagattgct gggcaacgcc ggcgtttcgc gtagttgctt ctgattacgt cggtactcca
     5041 ccatatgcat aaaagcagtg atggttcctt ccggcgtgcg ataggtcggc agcccggctt
     5101 cactgaataa tcgtcgtgcc tcttgcgagg agtgctcgcc gcaccagttc gtcagcagag
     5161 aaacatactt gctgcgggga tgatgcttta ccgcttcaat taatgcttgc gcactttctg
     5221 ttgcgggagc agcggcgctg ggcgaatgaa taaccatcag cgcgtcaaaa tcctggctgt
     5281 ggagcagaat atccagcgtt ttaatatagt gctcactgct ggcgtcatcg cgtagatcga
     5341 gcgggttaga gattgccaca tgttctggca gtgcatcgcg cagtttctgg caggtttctt
     5401 cgcttagcgt tgccagcttg ccattgcgtg accataaggc atccagcgcc agcgcggcag
     5461 gcgcagcacc gttgctgata atcatcagcc ggtcgccacg cagcgggcgc atatggctga
     5521 gggtttccac cgccgaaaac agctcgtgag tatcctgtac ccgcaacaaa ccggcacgct
     5581 gaatagccgc atcccacgcc ggatccattc ctgccgtggt gttcaacaga cgttgagccg
     5641 ccgggctacg tccgctttta atcaccagaa tcggtttgtt gcgagaggca ctacgggccg
     5701 ccgaaacaaa gcgtcgtgcg tcgcttaact gttcgagata gagcaggatg gcgctggttt
     5761 tactgtcgcg ggccagatag tcgagcaatt catcaacgtc gatatccagg ctgtcgccga
     5821 gcgcaataaa gtaggaaaag cccatctcac gctgttgcgc ccagtcgagg atggtattgg
     5881 agacggcagc cgattgcgaa ataaacgcca gcttgccgcg tttaatcggc acaggcgaaa
     5941 agctggcatt cagaccttgc cagggagcca gtaaccccag actgtttggt cctagcaggc
     6001 gcatgttatg gcgcagggcg caggcgcgga gatcttcatg ttgcgatgcc ggggcggaaa
     6061 gaataatgca ggttttacag cctttctcgc cgagctcttc cagaagagca agattacggc
     6121 tggcattggt acataaaacc gcaaggtcgg gtgtaaaggg caagctggca atatccggcc
     6181 aggccaacac acccaacacc gctttccagg ctggcgtcac cgggagtacc ggtccgttaa
     6241 agcctcccgc cagcaggtta cgcatcatca ggtaacctgc tcgattggat ttcatcgatg
     6301 cgccaattac cgcaatcgat tttggtcgca gtagtgcttc cagtcctcgc tgactcatac
     6361 cggtctcccg cagaagtgac cgaatgattt taaacgcttt ctaactgttc tgctgtgatg
     6421 ctacccagat gttgcgtttt tcctgccaga tagcgcgttt taaaacgagt gaaatgctcg
     6481 cctaagcccg ttgcggcttc ggtatcgccg gccatatcta acagtgcgat ggctacctcg
     6541 gcagtacaat attggccttc agcctgggct tcacgcaggc gataggcaga aagccgggaa
     6601 agatcgacgg aaatgacggg aagattatcc agatacggac ttttacgaaa catcttgcga
     6661 gcttcaggcc aggtaccatc gagcatgata aatagcggag gcttaccggc aggtggtgtg
     6721 aagatcactt cccgttgctc atcagcatac gaggcgggaa agaccaccat tggctgatag
     6781 tccgggtttt gtaccagatc cagcaaatct agcgagggtt cggtacgcga ccattgaaac
     6841 gcaacggtat caggcaaaat atcagcaatc agacgcccgg tattactggg cttcattggc
     6901 tcggtgtcga acatcagcaa acagaagcga ctttttgctt gtgctggggt aattgtcgaa
     6961 cagagacata atgtctctgg caaaagacag cgttggcagc gacgaacgcg attaccgcgg
     7021 gcaagaaaag gacgtgttgc gcgcgcaata cgctcggcgc gtaactggag aacagcgttt
     7081 tcggtcataa gagagcgtcg aaaaaacgcc attgtcgcag aggagaaaac ggggcacaag
     7141 atgcgccccg gtaagattaa agagattcgt tcagccagct atcaaacggc gctttcggta
     7201 ctgcgccatt aagcatgtcg acaacctgac cgtttttgaa aatcatgatc gtcggtatac
     7261 tacgaattcc aaagcgactg ctcaattcac gttcagcttc ggtattcact ttcacaaagc
     7321 gcactttacc gctacgctct tgcgcgacat cttcaaaaat tggtgcgaaa ttacggcagg
     7381 ggccgcacca cggtgcccag aagtcgatca ccacaggtag atcatctttc agcaatttgt
     7441 cgagcgtttc accggtcgca ttaatcacct ctccgtcaaa caagtcgtga ccgcagcgtc
     7501 cgcattttgc cgcatcttcg atccgatcgt cgggaatgcg attgatggcc tgacaatggg
     7561 tacaaacggt attcataact aacctcggga tgagtaagga gacttatcgg caacgacgcc
     7621 ggtatgtttc taatatgtta catattatcg gggacattgt tccagacaac aatctgtttt
     7681 attcaatagg tatgatagtc gcaggctttg gtacgaaatc atggcgaacc gcgcggactt
     7741 cgggtaatct gcgcgcttcg cgcagcgctg gtggagaaaa gcatgaacga tgaaatgaaa
     7801 ggtaaaagcg gcaaggtcaa agtgatgtat gtccgcagtg atgatgattc tgataaacgt
     7861 acccacaacc cgcgtaccgg gaaagggggc gggcgtccag gaaaatctcg tgctgacggt
     7921 ggccgtcgcc ccgcccgcga tgacaaacag agtcagcccc gtgaccgcaa gtgggaagag
     7981 tcgccgtggc gcacagtttc ccgcgcgccg ggtgatgaga cgccggaaaa ggccgatcac
     8041 ggtggcatca gtggtaaaag ttttattgat ccggaagtgt tgcgtcgtca gcgtgcggaa
     8101 gaaacccgcg tctacggcga aaatgcctgt caggcactgt tccagagccg tccggaagcg
     8161 attgttcgcg cctggtttat ccagagtgta acgccgcgtt ttaaagaagc cttgcgctgg
     8221 atggcagcaa accgcaaagc gtaccatgtg gtagatgaag cggaactgac aaaagcgtca
     8281 ggcacggaac atcacggcgg cgtttgcttc ctgatcaaaa agcgtaacgg tacaaccgtg
     8341 ctgcagtggg taagccaggc aggcgcgcag gattgtgtac tggcgctgga aaacgaatct
     8401 aacccgcata acctgggtgg catgatgcgc agctgcgcgc acttcggcgt gaaaggcgtt
     8461 gtggtgcagg atgcggcact gctggagtcg ggggcggcta tccgtaccgc agaaggcggc
     8521 gcagagcacg ttcagccgat tactggcgac aacattgtta acgtgctgga tgatttccgt
     8581 caggcaggtt acaccgtagt gaccacttcc agcgagcagg ggaaaccgct gttcaaaacc
     8641 agtctgccag cgaaaatggt actggtgctg ggtcaggaat atgaagggtt accggatgcc
     8701 gcacgcgatc cgaacgattt gcgcgtgaag attgatggta ctggcaacgt tgccgggcta
     8761 aatatctctg ttgcaaccgg cgttttgctt ggtgaatggt ggcgtcagaa taaagcctga
     8821 taaatcagcc gggtggcaac gctgccatcc ggcgttttcc tgcaaattta ctcactttct
     8881 gccggtaata ctggcatcca gtcaatcggt ttctcgccac gttgttccag ccactgattt
     8941 gccagcacaa aatggttgca gccaaagaac ccacgatgcg cagaaagcgg tgatggatgc
     9001 ggcgctttca gtatgtgatg gcgttgctta tcaataatcg cccctttctt ttgcgcatgt
     9061 gagccccaca acaaaaacac cacgtcttcg cggtgctggt taatcaggct gattacttta
     9121 tcggtgaagg tctcccagcc gaggctggcg tgggaatgtg cctgacctgc gcgaaccgtc
     9181 aacacagtat tgagtagcag aacgccctga cgcgcccagc tttcaagata accatgatta
     9241 gggcgggtga aaccgggaat agtattttcc agctctttat acatattcag cagtgacggc
     9301 ggtgtcgcaa tgccggggcg aacggaaaat gccagaccat gcgcctgtcc cggtccgtga
     9361 taaggatcct ggccgagaat caccacttta acgtcaccca actctgtaaa gcggaacgcg
     9421 ttaaagacat ctttttgtgg cggatagata gtgacgccgg actgccgctc gctggcgacg
     9481 gtctgtaggg tattaagaaa atagggttgc tgcttctctt cagccagcac atcatgccag
     9541 gttaattcgt ttgccatctc gctctcccgc gaatcttcaa tccgcctagc ttaactgctt
     9601 ctttctacag aacaaaatca tgctcatttt ttagacttaa acgagtgcaa aaaaatgtaa
     9661 attggtaaaa tttcacacct gcataagttg atgtaaaaca ataaaatcca tttgcaacca
     9721 tacttgatgt gtggttttta ttgatttaaa tcaaagattc aagggtgttt gaggagtata
     9781 tatacactca agcaacaatg gttttaccaa ttggccgcga caggctgaac aaatcaaata
     9841 attttgccgg ggaggcatca catgattaca ggtatccaga ttactaaagc cgctaacgac
     9901 gatctgctga actctttctg gctgctggac agcgaaaaag gcgaagcgcg ttgcatcgtt
     9961 gcgaaagcag gttttgcaga agacgaagtg gttgcagtaa gcaaactggg tgacattgag
    10021 taccgtgaag ttccagtaga agtgaaacca gaagttcgcg ttgaaggtgg tcaacatctg
    10081 aacgtaaacg ttctgcgtcg tgaaactctg gaagatgcag ttaagcatcc ggaaaaatat
    10141 ccgcagctga ccatccgtgt atccggttat gcagttcgct ttaactctct gactccggaa
    10201 cagcagcgcg acgttattgc tcgtaccttt actgaaagcc tgtaaggctt aagtagcaag
    10261 ttactaagcg gaagagggcg acttcttccg ctttttttcg ttaatagaaa atgcgtaccg
    10321 cgcaatagac cagcagcagc gcgagcacga tatttaactg gcgaccatac tggcgaaaca
    10381 atcgctgaaa caggtgcccc gccagcgccc agcacacatt gccaaacgtc ccaatcatcg
    10441 ccagcaaaac gctgacgcca attatccagc ttagcgcctg cgtttgcggc aggacaaacg
    10501 tcgacagtgc tgtaatgcca tacaaaatga ttttgacgtt cacaaactgc aaagcaaagc
    10561 tggcccaaaa actgattggt tttgtctgaa gcccatcttc ctttgttgga ctggtagcga
    10621 ttttccacgc cagccagaca atatatgccg cccccgccca actcaacaga tgcactgctg
    10681 ccgggtcgat aacagccagt gaaaacgaaa ttcccgcgca tagcaacatg acgattaaaa
    10741 atcccagact catccctgcc agcacgcgag tactctgacg aaatccgtgc gttgtggctg
    10801 agctaagggc gagaatattg ttcggtcctg gcgtcatggc ggtaatcagg gtgtaagtcc
    10861 aaaaagcact taaaagggtc ggtgtcactt gttatctcct ctgttttggt agacagtacc
    10921 cgacacggaa catttaaaaa aatgaatgtt tttacattct aaattcgata ttttcgatag
    10981 gtttggggtt atggatctgc gccgttttat tacacttaaa accgtggtgg aagagggttc
    11041 ctttttgcga gcttcgcaaa aattgtgctg cacacaatcg acggtgactt ttcatattca
    11101 gcagcttgag caggagttct cagtccagtt atttgagaaa attggtcgac gaatgtgcct
    11161 tacccgcgaa ggaaaaaagc tgctgccgca catttatgaa ctcaccagag tgatggatac
    11221 ccttcgtgag gctgccaaaa aagagtcgga tccggacggg gaactacgcg ttatttcagg
    11281 cgaaacgttg ctctcttatc gaatgccgca ggtgttgcag cgttttcggc aacgcgctcc
    11341 aaaagtgcgt ttatcgttgc agtcgctgaa ctgttacgtg atccgtgatg cgctactgaa
    11401 tgatgaggcc gatgtcgggg ttttttatcg cgtagggaat gatgatgctt tgaaccgacg
    11461 agagttgggt gaacaaccgc tggtgctggt ggcttcaccg caaattgcag atgtcgattt
    11521 tactgagccg ggaagacata acgcctgtag ctttattatc aacgaaccac aatgtgtctt
    11581 ccggcagata tttgagagca cgctgcgcca gcgacggatc acggtggaaa acaccattga
    11641 gcttataagt atcgaaagca tcaagcgttg tgtcgcggca aatatcgggg tcagttacct
    11701 gccgcgcttt acagtggcaa atgaacttgc gagtggcgaa ttaatcgaat tgccttttgg
    11761 cgaacagtcg cagaccatta cggccatgtg cgcacaccat gctggaaaag cggttagccc
    11821 ggcgatgcac acttttattc agtgtgttga agagtgtttt ttgccgggat aaaaacaatg
    11881 ccgggcttaa acccggcatt gagattactc ttctgtcgtt tgcggtggca cgcccgttcc
    11941 gcttggttta cggcgcttac caatattttt agtgtcgcga tggcgttttt tcacccgcgg
    12001 cttctctttc tctttagctt ttttcttctc ggcacgttta gccaacactt tcttcgatgg
    12061 cttgccggtc tgcttctcgc ttggcgcacg cgttttcgga cgtaactcat caattacgcg
    12121 agctttaatt ggctcttcaa tataacggcc aactttgccc agcagcagat ggtcatgggc
    12181 ttccaccagc gaaatcgcgg tgcctttacg accggcgcgc gcggtacgtc cgatacggtg
    12241 caaataagta tcgccactgc gcggcatatc gaagttaaag acgtggctga cgtcaggaat
    12301 gtcgataccg cgcgcggcaa catcggtagc aaccagtacg tttacgcggc cttcggtcaa
    12361 gcgtttaatc gcttcgttac gcttgccttg taccatctca ccttcgagat agcagttgtt
    12421 gatgcctgct tcgcgcagcc agtttgccag ctcatgcaca cgctcacgct tacgcacaaa
    12481 cacaattgag cgggtcgctt ccggctgttt taacagatgc accagcaacg cggttttatg
    12541 ctcaagatca tcggcgcggt aataccactg atgaattttt ttgcgctcac gggtggacgg
    12601 attggcagaa acctccaccg gatcttccag cagacgctcg gcaaagtcct gaatcgcatc
    12661 gccttccaga gttgccgaaa agagcagggt ctgtttacgc cagcgcgttt cgccagcaat
    12721 atgttcgata tcctgggcga agcccatatc cagcatacgg tctgcttcgt cgaggatcag
    12781 cgtttcaacc gcgcggcaat cgaagttctc ttcttttatg tattgcagca gacgtccggt
    12841 cgtggcgacc acgatgtcct gattttcgct gaacacttcc gcgtggttca tataggctac
    12901 gccgccggtg atggtggcaa tatccagatg cgtatgtttc gccagttcgc gggcatgatc
    12961 ggccacctgc atcgccagct cgcgagttgg ggtgaggatc aaaatacgcg gcggaccgga
    13021 tttcttacgt gggaaatcga gcaggtgctg caacgctggc agcagatacg ccgccgtttt
    13081 accggtgcct gtcggcgcag aaccgagtac atcacggcca tcgagcgcag gcggaatggc
    13141 ggcagcctga atggcggtcg ggcgagtgaa acctttatcc tggagggctt ccagcaggct
    13201 ttcgtcgagt tcaagttcgg aaaaagtcgt tacagtcatg ttctacctct gtgtggggcg
    13261 ctgattatag acgttacggc tgcaatcttc atctgtttgt atggatatcg cttctcgggt
    13321 attgttctca cccggtgcta tcgccgtttt tcctgcaagg ttttattttc atgtcacagt
    13381 ctacatccgt gcttcgtcgt aatggattta cttttaaaca gttttttgtt gctcacgatc
    13441 gttgtgcgat gaaagtggga acggatggca ttttgctggg cgcatgggca ccggtggctg
    13501 gggtaaaacg ttgccttgat atcggcgcgg gtagcgggtt gctggcatta atgctggcgc
    13561 agcgaaccga tgacagcgta atgattgatg cagttgaact ggaaagtgaa gctgccgcgc
    13621 aggcgcagga aaatatcaac cagtccccgt gggcagagcg gattaacgtc catacggcgg
    13681 atattcagca gtggatcaca cagcagacag tacgcttcga tttaatcatc agtaacccac
    13741 cttactatca gcagggagta gagtgcgcga cacctcaacg ggaacaggct cgctatacca
    13801 ctacgcttga tcacccatcg ttgctgacct gtgcggcgga gtgcattacc gaagagggat
    13861 ttttctgcgt ggtgctgcca gagcagattg gtaatggttt tactgaactg gcattaagta
    13921 tgggctggca tttacgtttg cggacggatg tggcggaaaa cgaagcgcga ctgccgcatc
    13981 gggtgctgct ggcattctcc ccgcaggcgg gagaatgctt tagcgatcgc ttagtcattc
    14041 gtgggccaga ccagaactat tccgaagcgt atacggcgct gacccaggct ttttatctgt
    14101 ttatgtaatg attgccgggg gaaaggatcg acggaccgga atgggtgagc agttccggat
    14161 aatccagcgt gaaatgcaac ccccgactct ctttacgcat cattgcacag cgaacaatca
    14221 actcggcaac ctgtaccaga ttacgcagct ccagcaaatt atttgagacg cggaaatggg
    14281 cgtaatattc gtctatttct tgctggagca tggttatccg ccgcagggcg cgttccaggc
    14341 gcttcgttgt gcgcacaatg ccgacgtaat cccacataaa cagacgtagc tcgtgccagt
    14401 tatgttgaat caccacccgt tcatcagggt tctcaacgcg gctttcatcc cacggcggta
    14461 acgtactgac gccgtgggca taaggcatac atctggtgat atcttctgcc gccgaccagc
    14521 catagaccag acactccagc aaggagttag aggccatgcg gttagcgccg tgtaagccgg
    14581 tataactcac ttcgccaatg gcatacaaac cctcgacgtc cgtacgccca tgatcatcca
    14641 ccatcacacc accgcaggta tagtgtgcag caggcacaat cggtactggt tcttgtgtga
    14701 gatcaatccc cagcccgagc aatttttcat aaatcatcgg gaaatgctgg cgaataaaat
    14761 cggcgggctt atggctgatg tcgaggaaca tacaatccgc gccgaggcgt ttcatttcat
    14821 ggtcaatagc gcgggcgaca atatcgcgcg gggccagttc gccgcgatca tcaaaatcgg
    14881 gcataaaacg cgtaccatcc gggcgcttga gataagcgcc ttcgccgcgc agtgcttctg
    14941 ttaacaggaa attgcgtgcc tgtgggtgat acagtgcggt agggtggaac tgattaaatt
    15001 cgagattggc aacccggcag cctgcgcgcc acgccatagc aatgccatcg ccagaagaaa
    15061 tatccggatt ggtggtgtac tgataaacct tcgacgcacc gccggttgcc agtaccactg
    15121 cttttgcgtg gcaggtttcc accgtttctt tatttcggtt ccatacccac gcgccaacaa
    15181 cccgtcgcgt gcccggcagg ccaattttgt cagaaacaat cagatcaacc gcgttgctgc
    15241 gctccagcac gcgaatattc ggatggttca gcgccttgct caccagcgtg gtttctactt
    15301 ctctaccggt ggcgtcggcg gcatgaagaa ttcgacggtg actatgtcca ccttcacggg
    15361 tcagatggta gctttctgca ccattcggtt gaatgtgggt atcaaacaac accccctggt
    15421 cgattagcca ttgcacacag gatcgtgcat tgctggcgac aaattcaact gcatgacgat
    15481 cgcaaatacc agccccggca atcaatgtgt cttccacatg cgagtcaatg ctgtcagtct
    15541 catcaaacac ggcggctata ccgccctggg cataaaatgt tgaaccttcc gttaccgggc
    15601 ctttacttag aacgatgacc tgatgctggt cagccaggcg tagcgccagt gaaagtccag
    15661 ctgcgccgct accgataatc aacacgtcac atgaatgttc agggagagta ttcattttct
    15721 ttgtttaatt tactaaacat ggtttggtca gcatagcatc atgttgtgcg attaaacacc
    15781 tgctatttta atatttgtta cagtgactaa acacgctgac tcagggcggc gagaaaaaga
    15841 gaagttactg gctggtggag gattaggtgg tgaaataaaa aggccgttgg gttactcttc
    15901 aggcagttaa atgggcattt ctacacagat aatgcgatgt tcagattctg tagacttata
    15961 atgatagata atgatccgtc tacagcatga caaacaaaaa cagatgcgtt acggaacttt
    16021 acaaaaacga gacactctaa ccctttgctt gctcaaattg cagctaatgg agtggcgttt
    16081 cgatagcgcg tggaaatttg gtttggggag actttacctc ggatgagcga gcagttaacg
    16141 gaccaggtcc tggttgaacg ggtccagaag ggagatcaga aagcctttaa cttactggta
    16201 gtgcgctatc agcataaagt ggcgagtctg gtttcccgct atgtgccgtc gggtgatgtt
    16261 cccgatgtgg tacaagaagc ttttattaaa gcctatcgtg cgctggattc gttccgggga
    16321 gatagcgctt tttatacatg gctgtatcgg attgctgtaa atacagcgaa aaattacctg
    16381 gttgctcagg ggcgtcgtcc accttccagt gatgtggatg ccattgaagc tgaaaacttc
    16441 gaaagtggcg gcgcgttgaa agaaatttcg aaccctgaga acttaatgtt gtcagaagaa
    16501 ctgagacaga tagttttccg aactattgag tccctcccgg aagatttacg catggcaata
    16561 accttgcggg agctggatgg cctgagctat gaagagatag ccgctatcat ggattgtccg
    16621 gtaggtacgg tgcgttcacg tatcttccga gcgagggaag ctattgataa caaagttcaa
    16681 ccgcttatca ggcgttgacg atagcgggat actggataag ggtattaggc atgcagaaag
    16741 aacaactttc cgctttaatg gatggcgaaa cgctggatag tgagctgctt aacgaactgg
    16801 ctcataaccc agaaatgcag aaaacctggg aaagctatca cttaatccgt gactcaatgc
    16861 ggggtgatac tcccgaggtg ctccatttcg atatctcttc acgcgtgatg gccgccattg
    16921 aagaagagcc agtacgtcaa ccggcgacat tgatcccgga agcccagcct gcgccgcatc
    16981 aatggcagaa aatgccattc tggcagaaag tacgtccgtg ggcggcacag cttacccaaa
    17041 tgggcgtagc cgcatgcgta tcgcttgcag ttatcgttgg cgtccagcac tataatggac
    17101 aatctgaaac gtcccagcag cccgaaacgc cggtatttaa tacactgccg atgatgggta
    17161 aagccagccc ggtaagcctg ggagtacctt ctgaagcgac cgcaaacaat ggtcaacagc
    17221 agcaggtaca ggagcagcgt cgtcgcatta atgcaatgtt gcaggattac gaactgcaac
    17281 gccgactcca ctctgaacag cttcagtttg agcaggcgca aactcagcaa gccgctgtac
    17341 aggtgccagg aattcaaact ttaggaacgc aatcgcagta atgaagcaac tttggtttgc
    17401 catgtcatta gtgacaggta gcctgttatt ctctgctaac gcctcggcca ctcccgcgtc
    17461 cggggcgtta ttacagcaga tgaacctggc cagtcagtca ctgaattacg agctgtcatt
    17521 catcagcatc aataaacagg gtgttgagtc tctgcgttat cgacatgcac gcctcgataa
    17581 ccgtcctctt gcacaattgt tgcaaatgga tggcccgcgc cgggaagtgg tacagcgcgg
    17641 caatgaaatc agctattttg aaccgggact tgaaccgttc acgcttaatg gcgattacat
    17701 tgttgattct ctaccatcgc tcatctatac cgatttcaaa cgcctttctc cttactacga
    17761 ctttatctcc gtcgggcgca cgcgtattgc tgatcgtctt tgcgaagtca ttcgtgtggt
    17821 tgcccgagat ggtacacgct acagctacat cgtgtggatg gacaccgaat cgaaattacc
    17881 gatgcgggtt gatctccttg atcgcgatgg tgaaacgctg gaacaatttc gcgtgattgc
    17941 ttttaatgtc aatcaggata tcagcagcag tatgcagacg ctggcgaagg caaatttgcc
    18001 gccgttgctt tctgttcctg taggtgaaaa agctaaattc agctggacgc caacctggtt
    18061 gccacagggt tttagcgaag tttccagtag tcgacgtccg ctaccgacga tggacaacat
    18121 gcctatcgaa tcacgtctct attccgacgg attattcagc ttctcggtaa acgttaaccg
    18181 cgctacgcca tcgagcaccg atcagatgtt gcgcaccggg cgcagaaccg tcagtacaag
    18241 cgtacgtgat aacgccgaaa tcaccattgt cggtgaactg ccgccgcaaa cggcgaaacg
    18301 cattgccgag aatattaagt tcggggcagc gcaatgatca aagagtgggc taccgtcgtc
    18361 tcctgggaaa acgggcaggc gctggttagt tgtgatgtta aagcctcatg cagcagctgc
    18421 gcttcacgtg ccggttgcgg tagccgcgtg ttgaataaac ttggcccgca aaccacgcat
    18481 accattgtcg taccctgtga tgagccgtta gtgccggggc aaaaagtaga attaggaatc
    18541 gccgaaggca gcctgcttag ctccgcatta ctggtttata tgtcgccgtt ggtgggacta
    18601 ttcctcatcg cttcactatt tcagctactc tttgcttcag atattgcagc attatgcggt
    18661 gcgattctcg gtggcgttgg tggattcctg attgcacgtg gctgctctcg aaagtttgct
    18721 gcccggtctg aatggcaacc gatcatctta agcgtggcct tgccgcctgg ccttgtgcga
    18781 tttgaaacat cttcggaaga cgcgagccag tgatttcact ggctttgttg cattttgcat
    18841 aacaaatgcg aggacgtttc cagataccac aatccatacg gtatgaacat ttcctcgcct
    18901 caatgttgta gtgtagaatg cggcgtttct attaatacag acgttaagct cagaacagcg
    18961 acttctaaaa gcctggttaa ccgggcatta aggcacaata atcatacttt atgaagaata
    19021 tacgtaactt ttcgatcata gctcacattg accacggtaa atcgacgctg tctgaccgta
    19081 ttatccagat ctgcggtggc ctgtctgacc gtgaaatgga ggcgcaggtt ctcgattcca
    19141 tggatcttga gcgtgagcgt ggcattacca tcaaagcgca aagcgtgacg ctggactaca
    19201 aagcgtctga cggcgaaacc tatcagctta actttatcga caccccgggc cacgtagact
    19261 tctcctatga agtttctcgt tcgctggctg cctgtgaagg tgcattgctg gtggtcgacg
    19321 ccgggcaggg cgtagaagcg caaaccctgg caaactgcta caccgccatg gaaatggatc
    19381 tcgaagtggt gccggtactg aacaagattg acctgccggc agccgatcct gaacgcgtgg
    19441 cggaagaaat tgaagatatc gtcggcatcg acgccaccga cgcggtgcgc tgttcagcga
    19501 aaaccggcgt tggcgtacag gacgttctcg aacgtctggt gcgcgacatt ccgccgccgg
    19561 aaggcgatcc ggaaggcccg ttgcaggcac tcattatcga ctcctggttc gacaactacc
    19621 tgggcgttgt ttcacttatc cgtattaaaa acggcaccct gcgtaagggc gacaaagtga
    19681 aagtcatgag taccgggcag acctataacg ccgaccgtct gggcatcttc acgccgaagc
    19741 aggttgaccg tactgaactg aaatgcggcg aagtaggctg gctggtatgt gcgattaaag
    19801 atatccacgg cgctccagta ggcgatacct taacgctggc gcgtaatccg gcagaaaagg
    19861 cgctgcctgg ctttaagaaa gtcaaaccgc aggtatacgc cggtctgttc ccggtaagtt
    19921 ccgacgacta tgaagccttc cgtgacgcgc tgggcaaact cagcctgaac gatgcttcac
    19981 tgttctatga gccggaaagc tccagcgcgc tgggctttgg tttccgctgc ggcttcctcg
    20041 gcctgctgca catggagatc atccaggaac gtctggaacg tgaatacgat ctggatctga
    20101 tcaccactgc gccgaccgta gtgtatgaag ttgaaaccac gtcaagagaa gttatctacg
    20161 tcgacagccc atccaagctg ccagcggtaa ataacattta cgaactgcgc gagccgattg
    20221 cagagtgtca catgctgctg ccgcaggcat atctcggcaa cgttattacg ctgtgcgtag
    20281 aaaaacgcgg cgtgcagacc aatatggttt accacggtaa tcaggtggcg ctgacgtacg
    20341 agatcccgat ggcggaagtg gtgctcgact tcttcgatcg cctgaaatct acctcgcgtg
    20401 gttatgcgtc tctggattac aacttcaaac gcttccaggc gtccgacatg gtacgtgtag
    20461 acgtattaat caacggtgaa cgtgttgatg cgctggcgtt gatcacccac cgtgataatt
    20521 cgcaaaaccg cggtcgcgag ctggtggaga agatgaaaga tctgatccca cgccagcagt
    20581 ttgatatcgc cattcaggca gcgattggta cgcacatcat tgcgcgctcc accgtgaaac
    20641 agctgcgtaa aaacgtactg gctaaatgtt atggcggcga tatcagccgt aagaaaaagc
    20701 tgctgcagaa gcagaaagaa ggtaagaaac gcatgaagca gatcggtaac gtcgagctgc
    20761 cgcaggaagc gttcctcgcc attctgcacg tcggcaaaga caacaaataa cccttaggag
    20821 ttggcatggc gaatatgttt gccctgattc tggtgattgc cacactggtg acgggcattt
    20881 tatggtgcgt ggataaattc tttttcgcac ctaaacggcg ggaacgtcag gcagcggcgc
    20941 aggcggctgc cggtgactca ctggataaag caacgctgaa gaaagttgcg ccgaagcctg
    21001 gctggctgga aaccggtgct tctgtttttc cggtgctggc tatcgtattg attgtgcgtt
    21061 cgtttattta tgaaccgttc cagatcccgt caggttcgat gatgccgact ctgttaatcg
    21121 gtgattttat tctggtagag aagtttgctt atggcattaa agatcctatc taccagaaaa
    21181 cgctgatcga aaccggtcat ccgaaacgcg gcgatatcgt ggtctttaaa tatccggaag
    21241 atccaaagct tgattacatc aagcgcgcgg tgggtttgcc gggcgataaa gtcacttatg
    21301 atccggtctc aaaagagctg acgattcaac cgggatgcag ttccggccag gcgtgcgaaa
    21361 acgcgctacc ggtcacttac tccaacgtgg aaccgagcga tttcgttcag accttctcac
    21421 gccgtaatgg tggggaagcg accagcggat tctttgaagt gccgaaaaac gaaaccaaag
    21481 aaaatggaat tcgtctttcc gagcgtaaag agacattggg tgatgtgacg caccgcattc
    21541 tgacagtgcc gattgcgcag gaccaggtgg ggatgtatta ccagcagcca gggcaacaac
    21601 tggcaacctg gattgttccg ccgggacaat acttcatgat gggcgacaac cgcgacaaca
    21661 gcgcggacag ccgttactgg ggctttgtgc ctgaagcgaa tctggtcggt cgggcaacgg
    21721 ctatctggat gagcttcgat aagcaagaag gcgaatggcc gactggtgtg cgcttaagtc
    21781 gcattggcgg tatccattaa tagccatctt cgttcacgtt gtcgccgtta tggcgacaac
    21841 gtgaattatt tatgagataa atctcccgtg gctaacgaca tcccccgtcg ttgtgtatag
    21901 aatattcccc cgaagtttaa ggttggcacc tccaggttgc cacggcacac gaaacagcgt
    21961 tggtccccat ataccggtaa actgaaactg cagtgaagca gttagccgag ccatgtatat
    22021 caggtctgtt tcgtgtgctg aattgttgac gcatttattt attggtatcg catgaacccc
    22081 atcgtaatta atcggcttca acggaagctg ggctacactt ttaatcatca ggaactgttg
    22141 cagcaggcat taactcatcg tagtgccagc agtaaacata acgagcgttt agaattttta
    22201 ggtgactcta ttctgagcta cgttatcgcc aatgcgcttt atcaccgttt ccctcgtgtg
    22261 gatgaaggcg atatgagccg gatgcgcgcc acgctggtcc gtggtaatac gctggctgaa
    22321 ctggcgcgcg aatttgagtt aggtgagtgc ttacgtttag ggccaggtga acttaaaagc
    22381 ggtggatttc gtcgtgagtc aattctcgct gacaccgtcg aggcattaat tggtggcgta
    22441 ttcctcgaca gtgatattca aaccgtcgag aaattaatcc tcaactggta tcaaactcgt
    22501 ctggacgaaa ttagcccagg cgataaacaa aaagatccga aaacgcgtct gcaggaatat
    22561 ttgcagggtc gccatctgcc gctgccaact tatctggtag tccaggtacg tggcgaagcg
    22621 cacgatcagg aatttactat ccactgccag gtcagcggcc tgagtgaacc ggtggttggc
    22681 acaggttcaa gccgtcgtaa ggctgagcag gctgccgccg aacaggcgtt gaaaaaactg
    22741 gagctggaat gagcatcgat aaaagttact gcggatttat tgctatcgtc ggacgtccga
    22801 acgttggcaa atccacattg ttgaacaaac tgctggggca gaagatctcc atcacctccc
    22861 gcaaggcgca aacaactcgt caccgcattg tggggatcca tactgaaggc gcgtatcagg
    22921 cgatctacgt tgatacccca ggcctgcata tggaagaaaa acgcgccatt aaccgcctga
    22981 tgaacaaagc ggcgagcagc tctatcggcg acgttgagct ggtgattttt gtcgtagaag
    23041 gcacccgctg gacgccggac gatgaaatgg tgctcaacaa actgcgcgat ggcaaagcgc
    23101 cggtaatcct cgcggtgaac aaagtggata acgtgcagga aaaagccgat ctgctgccgc
    23161 acttacagtt cctggcaagc cagatgaact tcctggatat tgtgccgatc tctgccgaaa
    23221 ccgggctgaa tgtcgacact atcgcagcaa tcgtgcgtaa gcatctgcct gaagcgaccc
    23281 atcacttccc ggaagattac atcaccgatc gctcacagcg ttttatggcg tctgaaatca
    23341 tccgcgaaaa actgatgcgt ttcctcggcg ctgaactgcc gtactccgtg accgtggaga
    23401 tcgaacgttt cgtctctaac gaacgcggcg gttatgacat caacggtttg attctcgttg
    23461 agcgtgaagg gcagaagaag atggttattg gcaacaaagg ggccaagatc aaaaccatcg
    23521 ggattgaagc gcgtaaagac atgcaggaaa tgtttgaagc gcctgttcac cttgagctgt
    23581 gggtaaaagt gaaatccggt tgggccgacg acgaacgcgc actgcgcagt ctcggttatg
    23641 ttgacgatct ttaagagtaa ctccgatgga aggctggcag cgcgcatttg tcctgcatag
    23701 tcgcccgtgg agcgaaacca gcctgatgct ggacgtcttc acggaggaat cgggtcgcgt
    23761 gcgtctggtt gccaaaggcg cacgctctaa acgctctact ctgaaaggtg cattacaacc
    23821 tttcacccct ctcttgcttc gcttcggtgg gcgtggtgaa gtcaaaacac tgcgcagtgc
    23881 tgaagctgtc tcgctggcgc tgccattaag cggtatcacg ctttacagcg gtctgtacat
    23941 caacgaactt ctctcccgcg tactggaata cgagacgcgc ttctctgaac tctttttcga
    24001 ttacttgcac tgcattcagt cacttgcagg tgccactggt acgccagaac ccgcgctgcg
    24061 tcgctttgaa ctggcactgc tcgggcatct gggctatggc gtcaatttta cccattgtgc
    24121 gggtagcggc gagtcggtag atgacaccat gacgtatcgt tatcgcgaag aaaaagggtt
    24181 tatcgcaagc gtcgttatcg acaataaaac gttcaccgga aggcagttaa aagcgttaaa
    24241 cgcgcgggaa tttcctgacg cagacacact gcgcgccgcg aaacgcttta cccgcatggc
    24301 gcttaagccg tatcttggcg gtaaaccatt aaagagcagg gaactgttcc ggcagtttat
    24361 gcctaagcga acggtgaaaa cacattatga atgatgagga ttgtcatggc tgaattactg
    24421 ttaggcgtca acattgacca tattgcaacg ctgcgtaacg cacgcggtac tgcttacccg
    24481 gatccggtgc aggcggcgtt tattgctgag caggcgggag cggacggcat taccgtgcat
    24541 ttacgtgaag atcgccgcca cattactgac cgcgatgtgc gcatcctgcg tcagacgctg
    24601 gatacccgca tgaatctgga gatggcggtg accgaagaga tgctggcgat cgccgttgag
    24661 acgaagccac atttttgctg tctggtaccg gaaaagcgtc aggaagtaac aaccgaaggc
    24721 ggcctggacg tcgcagggca gcgtgacaaa atgcgcgatg cctgtaaacg tctggcggag
    24781 gccgggatcc cggtttctct gtttatcgac gccgatgaag agcagatcaa agctgcggca
    24841 gaggttggcg caccgtttat cgagatccac accggttgct atgctgatgc caaaactgac
    24901 gccgaacagg cgcaagagct ggcgcgtatc gccaaagccg cgacctttgc cgctagtctc
    24961 ggtctgaaag ttaacgctgg tcacggtctg acctatcaca acgtgaaagc tattgccgcc
    25021 atccctgaga tgcatgaact gaatatcggt catgccatta ttggtcgtgc agtgatgacc
    25081 ggactgaaag atgcggtggc agaaatgaag cgtctgatgc tggaagcgcg tggctaatgg
    25141 caatattagg tttaggtacg gatattgtgg agatcgctcg catagaagcg gtgatcgccc
    25201 gatccggtga gcgcctggca cgccgcgtat taagcgataa cgaatgggca atctggaaaa
    25261 cgcaccacca gccggtgcgt tttctggcga agcgttttgc tgtgaaagaa gcggcggcaa
    25321 aagcgtttgg caccgggatc cgcaatggtc tggcgtttaa tcaatttgaa gtattcaatg
    25381 atgagctcgg caaaccacgg ctacggctat ggggtgaggc attaaaactg gcggaaaagc
    25441 tcggcgttgt aaatatgcat gtaacgctgg cagatgaacg ccactatgct tgtgccacgg
    25501 taattattga aagttaaaaa aataccgttc tctttataaa gtaaaacccc cgaactggta
    25561 aggaacgggg gttttcactt tacaacttta attttgtgca gcacacgatt ccaacagaaa
    25621 tgattatgga tcaatttcgt tatcctgtac aagcaacttg caatctctga cataaaaaac
    25681 gcgagccttt acgaagaaat cgacattgat aaaaagatta acgtgaccgc tttttgataa
    25741 gtacattaaa atcggtttcg ttattggcaa ttttgctgtg ctttacacca tgccactgaa
    25801 ttcccccatt gaaacgagtg gtgtcgtcaa agctctggtg tggagtgcag cactcgattc
    25861 caataactcg cacgttcagt tttggggtga tgtaagggct aatctgaacg gctgcattcc
    25921 ttgtttaagg aaaaacgaat gactgattgc cgatacctga ttaaacggat tatcaaaatc
    25981 atcattgctg ttttacagct gatccttctg ttcttataac acaaggaaac gtacttaagg
    26041 tgcgtccggt gaaccagtcg gacgcgcctt taataactat a
//
LOCUS       DAZGHF010000068        26013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGHF010000068 DAZGHF010000000
VERSION     DAZGHF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.068"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(27..4487)
                     /gene="mukB"
                     /locus_tag="ACHK3A_002849"
     CDS             complement(27..4487)
                     /gene="mukB"
                     /locus_tag="ACHK3A_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO8740905.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPYQAS"
     gene            complement(4487..5191)
                     /gene="mukE"
                     /locus_tag="ACHK3A_002850"
     CDS             complement(4487..5191)
                     /gene="mukE"
                     /locus_tag="ACHK3A_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO8740906.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5172..6494)
                     /gene="mukF"
                     /locus_tag="ACHK3A_002851"
     CDS             complement(5172..6494)
                     /gene="mukF"
                     /locus_tag="ACHK3A_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO8740907.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6491..7276)
                     /gene="cmoM"
                     /locus_tag="ACHK3A_002852"
     CDS             complement(6491..7276)
                     /gene="cmoM"
                     /locus_tag="ACHK3A_002852"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO8740908.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7412..8191
                     /gene="elyC"
                     /locus_tag="ACHK3A_002853"
     CDS             7412..8191
                     /gene="elyC"
                     /locus_tag="ACHK3A_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO8740909.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKSSSGEPRQE"
     gene            complement(8168..9061)
                     /locus_tag="ACHK3A_002854"
     CDS             complement(8168..9061)
                     /locus_tag="ACHK3A_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO8740910.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDTSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9215..9961)
                     /gene="kdsB"
                     /locus_tag="ACHK3A_002855"
     CDS             complement(9215..9961)
                     /gene="kdsB"
                     /locus_tag="ACHK3A_002855"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO8740911.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVTQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9958..10140)
                     /gene="ycaR"
                     /locus_tag="ACHK3A_002856"
     CDS             complement(9958..10140)
                     /gene="ycaR"
                     /locus_tag="ACHK3A_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO8740912.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10192..11424)
                     /gene="ycaQ"
                     /locus_tag="ACHK3A_002857"
     CDS             complement(10192..11424)
                     /gene="ycaQ"
                     /locus_tag="ACHK3A_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO8740913.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLGDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPEWNDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            complement(11461..12447)
                     /gene="lpxK"
                     /locus_tag="ACHK3A_002858"
     CDS             complement(11461..12447)
                     /gene="lpxK"
                     /locus_tag="ACHK3A_002858"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO8740914.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     NAGQTLVMTEKDAVKCRAFAEGNWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(12444..14192)
                     /gene="msbA"
                     /locus_tag="ACHK3A_002859"
     CDS             complement(12444..14192)
                     /gene="msbA"
                     /locus_tag="ACHK3A_002859"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO8740915.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14229..16493)
                     /locus_tag="ACHK3A_002860"
     CDS             complement(14229..16493)
                     /locus_tag="ACHK3A_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO8740916.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLASLTVFACVLVFIPF
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVAHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSQLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16700..16984)
                     /gene="ihfB"
                     /locus_tag="ACHK3A_002861"
     CDS             complement(16700..16984)
                     /gene="ihfB"
                     /locus_tag="ACHK3A_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO8740917.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17144..18817)
                     /gene="rpsA"
                     /locus_tag="ACHK3A_002862"
     CDS             complement(17144..18817)
                     /gene="rpsA"
                     /locus_tag="ACHK3A_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO8740918.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18928..19611)
                     /gene="cmk"
                     /locus_tag="ACHK3A_002863"
     CDS             complement(18928..19611)
                     /gene="cmk"
                     /locus_tag="ACHK3A_002863"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO8740919.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19784..20548)
                     /gene="ycaL"
                     /locus_tag="ACHK3A_002864"
     CDS             complement(19784..20548)
                     /gene="ycaL"
                     /locus_tag="ACHK3A_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO8740920.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(20717..22000)
                     /gene="aroA"
                     /locus_tag="ACHK3A_002865"
     CDS             complement(20717..22000)
                     /gene="aroA"
                     /locus_tag="ACHK3A_002865"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO8740921.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQDNYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNMMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRIAMCFS
                     LVALSDTPVTILDPKCTAKTFPNYFEQLARISQAA"
     gene            complement(22071..23159)
                     /gene="serC"
                     /locus_tag="ACHK3A_002866"
     CDS             complement(22071..23159)
                     /gene="serC"
                     /locus_tag="ACHK3A_002866"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO8740922.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSNFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(23358..24050)
                     /locus_tag="ACHK3A_002867"
     CDS             complement(23358..24050)
                     /locus_tag="ACHK3A_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO8740923.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFIILALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESVDYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            24180..25940
                     /gene="ycaO"
                     /locus_tag="ACHK3A_002868"
     CDS             24180..25940
                     /gene="ycaO"
                     /locus_tag="ACHK3A_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO8740924.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELAGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         6304 a         7084 c         6326 g         6299 t
ORIGIN      
        1 aaggcggcat tgctgccgcc ttcattttaa ctcgcttggt aaggcgcttc gtcacttcct
       61 ggaagcgttt cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacgtgttcg
      121 gtattctgga agactttacg caccagttta taggtggtgc ctttttccgg actgatattt
      181 tccggcgctg cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg
      241 atagaacgag catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata
      301 tctttaccgc gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc
      361 gacataccgg taccaatcgc ctcaccggtc gacagagcgc cagattctgc gcgcagccag
      421 ccatcagaac cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt
      481 tcttcgccaa tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga
      541 tacaatttcg ccagcgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc
      601 tgctcgtgct gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcaca
      661 ttcagacgta cgctgtttac ctgaccgaac gatacgttct gcaaaccctg gttgagcata
      721 cggatacggt tctgttcgcg ctgaatggtt ttgcgaataa tgttcgccac gctgcgggaa
      781 ctgatcgcca gtttctgttc acgggaggtt aactcttcgg tcagacggct aagttcgatc
      841 tccatctgtt cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt
      901 tcacgcagat gctgataaac cgccacgaag aactgaattt tacgttccgg acgttttgga
      961 tcttccgaca tccgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc
     1021 gcacctaacg ccttatccga catggaacgc aaatcatcag cggagagata agccagctca
     1081 cgacggtgta agcggcgttc aacgccgtta tctttcacca tgcgcatgac tgcacaccag
     1141 cccgctttcg cggtcactac ctgctcgcgc atctcaaagt aatcacgctc cagcttgcgc
     1201 agtttgcggg tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga
     1261 ttgcggcgtg aacggttatt gctcagttgc gcgtgcaact cgtcacggcg aatacgcgcc
     1321 cgctcttctg ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg
     1381 ttgagtagct cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac
     1441 tgactcaact gcgctgcgtg tccgcgcaac gcttcacgcg cgcgggtacg ctccgcttcc
     1501 gcctgttcca gacgttcacg cagtttttcg ttgagatcgc tgttaccgct cagcatttct
     1561 gccgagtcag agtagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgcc
     1621 tgctgacggg catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg
     1681 aactgttccg ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg
     1741 ccaaactgct gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca
     1801 tcgacgcgat ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc
     1861 agcgccgtaa cgccctcttt cgcctgctca aattgaatac gctgctgctg gttatcattt
     1921 tcatgattac tcagcgcccg ctccagttcg acgcgacggc tgttcagttg acggatttcg
     1981 gcttccggat cagactcaaa cgccaccgcc agatgactac ctataaagcg gctgaacgcc
     2041 tgatgcagac gctgggtttt ctgtacatca aaagagagcg tggcgaagcg ttcggaaagc
     2101 acttcacgct cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc
     2161 ggaacttccg gaaaacgtga gtaacgccac tgacgatcgg cgattttcac cactaccgct
     2221 ttttccagct catcaacgct gaacacgctg tcatcgaatg actgcggatc cccttcgatc
     2281 agatagagat cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga
     2341 tctggcacca cgatggcgtg gcgtgacggg ccatacagtg ccgagaagta cggcgcatct
     2401 tccagactca cgtcgtcata aatttcggac agcagcacgc cgccaaaacg ctccgccagc
     2461 gcattcaaac gctggtcttc agaaccacct ggctggctta aacgttcgat ctcttcatcg
     2521 acggcgtttt tgcgcgcgcc tacttcatcg cgttcaacaa tggcttcgcg ctcacgctcc
     2581 agcaactgtt gcagatattc agtgacgtcc tggctggagg tgaactcttc gccgcactgt
     2641 tcgctcaact ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa
     2701 ctctgaatgc gagactgcag ctgttcctgt tcctggcgca gtgccatccg ctcttctcgg
     2761 gcgttagaga cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct
     2821 tccagttcat caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc
     2881 tcagcttctt gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc
     2941 tgaacctgct ctgccaggtg acgctgatcg accccttcgc gcaatagttc gcgagcgaca
     3001 tcccacgcct cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc
     3061 tcaaactggc tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt
     3121 tcggtcgctt ccagctcttt cgcctggaag gtttccagcc attcggcagc gctgtcggcg
     3181 gttaagtccg gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta
     3241 tactggatcg cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt
     3301 ttcagctcgt ccacttccag ttcggcagct tccgcacgag cctcgttctc ttcctggcgt
     3361 tcgatggctt ctgccaccac ttcgttttgc tcttccagac ggatctgcag ctcatcgaga
     3421 tccgcttcgt agcgttcgat cttctcctgc tgacgcagtg cggtttgcac caggttcagg
     3481 tgatcgctgg ccgcctgata atccgcttcc agatcgcctt cggcaccgtt gtgctcagcc
     3541 agctctcgcg ccatgtcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta
     3601 tgtagctcgc gacgaaactc cagggcctta tcgagatgaa cacggcgctc gttggcgtga
     3661 cgcatgtagt ccgccgccac gtagttggtg gcttcgctga tcagatgctt aaacaggtca
     3721 cggtctgact gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct
     3781 tccatgtcct ggaacgcttt ccgcacccca ctgttttccg gcaacaggta gtcgcgcaga
     3841 gaacgagtaa tggcgctgga gatcccgcca tacagcgagg cttcgatcag acgatagaat
     3901 ttactacggt cagacgctga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc
     3961 gagtggtaat cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc
     4021 ttgtctttca gctcgttaag cggcagcacg cgcgcctggc gttcgttcag agtttcggtc
     4081 accagttgtg tcggctgcac cgacatcggc aggccctgaa tggcaaatgg tttgatatcc
     4141 actttacgat cgcgtccggc aacctgttgc agacgcacac cgaccactac acgctggtgg
     4201 cgcgagttaa tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga
     4261 cctttatcgc gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg
     4321 gtcaggtcgg ggataagcgc cgtaacgaac gctgccatgg tggtggattt acccgccccg
     4381 ttaccgccag aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg
     4441 ttccagttaa tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc
     4501 cgctatctgg ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca
     4561 ttgcttcgcc atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca
     4621 catcggctcc gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca
     4681 taaaccacac catacccaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca
     4741 acttctgccg gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg
     4801 cttcatcggc cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat
     4861 tcgccagccg ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca
     4921 gttccgacaa gacggaacga gggatcagcg tggtggaacg tgggcgtaga tagaagaacc
     4981 cttccggtgc acgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct
     5041 gaaaatccat caggaatgca tgattatcca gttcgtcgag gccaatatga cggcctgaac
     5101 gtaaggcgct atccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg
     5161 gcatcacttg ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta
     5221 atcggctgcc atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc
     5281 gcctgatcaa taacaatgcg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga
     5341 tattcgcgta ccaccagacc aagatccaac ggcacttgtc tggttttgta cacggcaagt
     5401 tgttcttcga tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtactccaga
     5461 tcctcaggaa gttccccagt cacttcttca tcgcgcagta ccatctcttc gtcacgcata
     5521 tccagcagac gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt
     5581 accgactgac gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcagtacgg
     5641 ataaatttgt gtacgtggcg gtcgtagcca atccacaagt ctatggattg ctggccccaa
     5701 ctgataatac ggtcgagttt gctctgcaga tcgaacacca gacgatcgac aaaatgcagg
     5761 tcgtcatggg tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttatcgcct
     5821 gccgcttcca gcgtatcctg caattcacgc agcgttccgg aagtttctga aagcaacaat
     5881 tcacagctgg aaatcgccgc ccgccagtct ttgttcaaca actgggcgat atcatccttc
     5941 acctgctgct gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct
     6001 gctaccgaat atttcagtgg cgcataaaca ttacggtgcc agtgaaattc atcaccgccc
     6061 tcttcggcgg catctgccgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa
     6121 aggcgcagcg tagaaaactc gcgctgacgg atgtaatagt cagtaatgcc gatgccgagc
     6181 ggcgtcagac ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga
     6241 cgttgacgca ccatgtcgtt gatcgcgttg ttggcgcgca cgccgatggt ttcgctggtt
     6301 tgctcaaacg catcactcac atggcggaat gcatccacca gctcgccttc actcatctca
     6361 ccatccagac gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc
     6421 ggcagcgaga tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg
     6481 gaaaattcac tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac
     6541 gccccagggt aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt
     6601 catagcagtc gcgctgctgg tgcttctcgc gcagataatc atgaaacacg cgaacgcctg
     6661 tcttacccat aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc
     6721 gtggataatc tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat
     6781 caaaattccc ggcgaccatg ttatgcatca gcaagccgtg cgcattgtag aacattaacg
     6841 acaacacacc gcctggacgc aacactgacc acagggtctg taatacgctg cggggatcgg
     6901 ccacccactc gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgtgaag
     6961 caacatcctg agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg
     7021 ccgcctgttt tgcgcggtcg atcatctgcg cagaaagatc gcataaaatg acctgatgcc
     7081 cgcgctcagc cattttgatt gcggtctgcc cttctccacc gccagcatcc agcacacgca
     7141 gtttttgcgg gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc
     7201 gaagctgccc tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa
     7261 aattgcgatc ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc
     7321 tattttgtca cagccacggc gagaatgaac ccgtctggtg taatatcgct ggcgatccct
     7381 tgctatatgg ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg
     7441 caatatgttg ctgccacttc cgctaatgtt gttgattatt ggcgctggcc tggcgctgct
     7501 gtggtttagc cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcgct
     7561 cttgttattg agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta
     7621 tccgacatgg aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac
     7681 ctggaacccg cagtgggcac caagctcgaa tttaatcaat aacagtctgc cccgcctgaa
     7741 tgaaggtatt cgcttatggc gcgaaaatcc gggatcaaaa ctgatcttca cgggaggcgt
     7801 agcaaaaacc aatacggtga gtacagccga agtaggtgcc cgagttgcgc aatcgctggg
     7861 cgtgccgcgc gagcaaatta tcactctgga tttgccaaaa gataccgaag aagaagctgc
     7921 ggcagtgaaa caggcgattg gtgacgcccc tttcctgctg gtgacatccg cgtctcattt
     7981 gccgcgggcg atgatctttt ttcagcagga agggttaaat ccgctgccag ccccggcgaa
     8041 tcagttggcc atcgactcgc cgcttaaccc gtgggaacgg gcaatccctt ctccggtctg
     8101 gttgatgcat agcgatcgtg ttggctatga aacgcttggc cgtatctggc agtggctaaa
     8161 aagttcgtca ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac
     8221 taaatcgtcc ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc
     8281 gccagatgaa cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg
     8341 ccagagaagt atccattagt cggaacagtt cgtattcccg cggtgcccaa agcattagtc
     8401 ccggcccgac catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac
     8461 agaagttacc gtgaatcagc acacaattgt cgttaaagcc ttcaaataat gccgggagac
     8521 attcgcgagt acgaaacagg atccgcttat cctgcatcgt tagaccggtg ttattgaact
     8581 gattgagcgt ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt
     8641 cctgagtatt gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta
     8701 aggcttcaac gatttgatct ttgagttgtt cccagcgttc tggtgttcgg gctggtgcct
     8761 ccaccgaaac accacgcatc cgctccagta gcaggacatc ggggccgggg tgttcttcat
     8821 gcgtcatcac accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg
     8881 ttttccatgc cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca
     8941 ttgggtttcc ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc
     9001 actcaatacg actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca
     9061 ttcagattac cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca
     9121 cctgaatgag atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc
     9181 ctcccaaaat tggctggagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct
     9241 cgagatcttc aggggtatcc acacctgtgc caggaacttc ctgggtgaca gcaacatgga
     9301 ttttttcgcc gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac
     9361 ttggctgcca gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat
     9421 gacgcaggaa gttatcgcca acggtttcaa ggccttttgc aaaacgatca cgatcccaag
     9481 gaatggtggc gcgagagaag tacagtgcat acccttcagc gtcgaggacc actttcaccg
     9541 cattcgggtt aaacgcttcc tccgcattgt ggattggcac cgccagagtc gccataccca
     9601 cctgacgctg agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt
     9661 catcaccctg cacattaacg atcacagtgt cgtcgctgaa tgcgcatttt tcgacaactt
     9721 ccgccaatcg ttctgtacct gactgatgat cggcgcgcgt catacatact tcgccgccag
     9781 cggcttcaac agcgcgggca acatcctcat gatcggttgc cacgatgatg cgctcggcac
     9841 ctgattcgcg cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca
     9901 atggtttacc gggcagacgc gtggacgcgt aacgcgcggg aataatgacc acaaaactca
     9961 tgatttactc tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc
    10021 atcgcgcagc gggaaagcca ggttgtccag tttgcaaata agctcttgtt tttcctggtt
    10081 ataccaaagt tttccgttgc aaaccgggca ggcaatgatt tcaagcagac gatgatccat
    10141 agttcctcca gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac
    10201 ggggtctatt tcccagccgg cgcggcaatc agtaaagagg ccttgcgggc agcgtcctaa
    10261 tgtcacccgc gttgcctgct gccagttagc gaaatcagta atcgcctgac gtaacccttt
    10321 ttgcagcgtt gtcgttggtt taatgccttc ttgtaaccac agagagataa cttcaaggat
    10381 gcctgtctgg cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa
    10441 cggcagaaca aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta
    10501 gctaaaatca aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga
    10561 aagtaccgcg ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag
    10621 caaatcagca tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg
    10681 ctgttcagcc ctcgcttcac gccacgccgc cagtgccgga cgttttagcc ggtagtaatc
    10741 tgccagccac tgttcgcgaa atatacccag gctacgcgca ctgttatcca gcatgatgat
    10801 ttctgcttct gcttgcgaaa cgagatcgcg ctcatcattc cactcaggca tgacacggtg
    10861 agttaaatca taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt
    10921 aaataaacct tccagatgtc gtttatgcgg cttccactcc caccagccgc tcgccccttt
    10981 acgaggatgc tcaaaatcgg ctgaacgtac tggcccctta tcatgaatat gctgaattaa
    11041 ctgtgcaatt tccgccgcat gttcctgcat ccaggcgtct ttatatttcc agcccatttt
    11101 ttcaggtgcc agcatgcggt ggcgaataag acgaaaatcg ctacgcggca taaagcaggc
    11161 ttcatgcgcc cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc
    11221 aggataattt cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat
    11281 ggtatcgatt tgcagcaagg acatgcggga gatcgttgcc ggaatatccc ccaacgacgc
    11341 tcgacggcgg ggtttgttta acaggccttg cgcggcgagg tgaagattac gcgcatcagc
    11401 aagagagagg tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa
    11461 ctagttgcca gaagccagcg aggttagttg cgcaagcagt ttcgctggtt catcacctga
    11521 aagctgtgcg tcaacaggca aataccacca atttccttcg gcaaaggccc ggcatttcac
    11581 cgcatctttt tcagtcatta ccagcgtttg cccggcgttt accaacgcgc tgacatccgc
    11641 atggttcaaa gactgatgat cggccagcgg tacacatttt tccggttgta cgccgcacat
    11701 cttcagcgtg gcaaaaaagc gcggcggatg tccaatcccc gccatcgcca ctacatgttc
    11761 aagctgagca acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag
    11821 atgcatgggg atttcaccgc tgcgggggac accgccgttg acgattaccg catcaaccga
    11881 ctttaagcgc cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa
    11941 gcgacgcaca ccatcaataa cgacaatttc cacatcacgc gccagacgat aatgctgtaa
    12001 accatcgtcg gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc
    12061 gtcagaacgc acgggagaaa ccgcaacagg cgcgccagtg cgttgataaa tcaacacagg
    12121 ttcatcaccc gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc
    12181 cttaccacca tatccccgcg atacaacccc cacgcgaata ccgcgctgtt gcagctgttc
    12241 caccagccag acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac
    12301 gacaaccggt acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat
    12361 cgcgccactc accaggccat acaaccagga gagtggcagc aatagccgcc ataaagggga
    12421 ttcaccagac cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa
    12481 acgccacggt gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg
    12541 acaaccacga tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag
    12601 gtacggtttt tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc
    12661 aacgccgagg tagcttcgtc cagaatcagt atcgggctat cacgcaacaa ggctcgagcg
    12721 atagcaatac gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta
    12781 tcgagaccgt tatccatctt attgatgaag tccatggcgt aggccatacg cgccgcttct
    12841 tcaatttgct cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta
    12901 tcgttaaaca gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg
    12961 gtatactcgc gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa
    13021 cgcgtgatca ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc
    13081 gtcttccctg ccggaatttt caggttgatg ttacgcaatg cgggcacatc acgtcccgga
    13141 taagtaaagg tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta
    13201 ccttcgtctt tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata
    13261 ccgcgctgga actgggcgtt aacgttggtc agcgatttca gcggacgcat aagtgcaatc
    13321 attgaagaga aaacaacggt aatcgtaccg gcagtcaggc tatccatgac acttgggaag
    13381 cttgccgcat acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca
    13441 gagatggaag aggctgaaac cattttcatc ccctgaagac gcattcggtt gctgacttta
    13501 tcgaagcgtt tcgtttccac ttcctgacca ccgaaaatca atacttcttt atggcctttc
    13561 agcatttgtt cggcgctggt ggtcacctgc cccatggtgt tctgcatgtt tttactgatg
    13621 ttgcgaaaac gcttcgatac aacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc
    13681 aaaatgatcg acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac
    13741 gcaccttcac gtacaacagt aatcagtgcg ccggaagaag aagaagcgac ctgttcggaa
    13801 tcgtaggtga tacgtgacaa cagcgtaccc gttgactgtt tgtcaaagaa tgaaaccggc
    13861 attcccatca tgtgaccaaa caggcggcga cgcatagtca ttaccacctt tcctgatacc
    13921 caggagatac agtagctgga gatatagctg gtgataccac gtaaaatcat cagcccgatc
    13981 accaccagcg gcatccacat cagcacggag cgatctgttt taccaaagcc atcatcaaga
    14041 agtggcttaa ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg
    14101 cccgccacga tcagacccgc tttaaaaggc gcaatggttg gccacagtcg gcggaatgtc
    14161 tgccacgtag agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc
    14221 cgcatattct acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa
    14281 atttgctctc tgaggctgct aatccgccag ccttgcgctg aaaaattgac cgttatttga
    14341 ccctgatgtg gagtatcaag ccacttataa ccctgttgtt gatagcgatg tttaactttg
    14401 cttgagggca gtcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc
    14461 actcgctgaa ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagt
    14521 aatgttgcct gcatttgctg ccagtaacgg cttagcatct tttgttcagc gggggcttca
    14581 atatcaccgg ttagaagaat gctattcgtc ccgtcatcaa tcttaaccac acaggaatgg
    14641 ttatttcctt tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc
    14701 cattgccacg cttcgccacg cacacagggc tgatgatgtt cccagtttaa ctgacttcgg
    14761 atccataaca tcggccatgt gtgcaatatt gagtccagcc ctccccggtg atccagatgt
    14821 tcatgactca aaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc
    14881 agttgttgcc cactatcccc ttcgggccag gccagtcccg tgtcatagag aatcgctttg
    14941 ccgtttcgcg caatgaccat tgccagccct tgcccgacat caagcatgta cacttgccac
    15001 tcgtcaggtc gaggtttttg ccacagcggc aagcacatca gcaagcctac agccacacac
    15061 attgctggca acgttcgcca ggcgtttaat cgccatacca ccagtaagaa ccatggggaa
    15121 aatgttagcc attgccaacg ttcagcaatg ttgatccacc cttccggcaa actctttaac
    15181 ccccagaaaa gtaaagccaa agaccggtcg gcaagaaacc ataacccttg ttccagaata
    15241 aacggcccgc ttaaatgcgc aaccatcgcg gcgaggatca acggaaccgt gataaatgtc
    15301 accaggggaa ttgcaaatag atttgcaata aacgaggtca gactaatgcc atgaaatatg
    15361 acgatttgca cgggcataag caggagtgtg attcccagtt gtaaatgaat gagggaaaca
    15421 actgcacgca ataccggcgg cagttgccac tccggacagg gaaaccactg gtaccaaaaa
    15481 atcagtgccg cgaccgcagc ggcagagagc cataaacttt gcgagagaat ggcaacagga
    15541 tccatcagca aaattgccgc cagacaacat atccatacat cccagccact ccattgtcgc
    15601 ccacttaact taagcattcc ccacatggca agcgccacca cggtacgcaa tgcaggaggt
    15661 tgcatcccag tcagccaggc ataaaaagca gcacagcaga ttccgccaat taatggcatt
    15721 tgccagtgga tccagcgccc aggcagaaaa acttgcccac cgcgaatgag tccggcagcc
    15781 agcaacgccg caaaagcgat atgcaatccc gatatcgcca ttaaatgcgc cgttccagta
    15841 tcgcgcatga tatttttgat ttctttggga acggataacc gttcccccat acctaaacca
    15901 agaataaccg cgttccacat atagggttgc agcgttgttt gtagtgacgc cagatactgt
    15961 gcacgcaggc tacaattcgg ttcaatgaca cttgcctgca gaaagcggcc ggtgagcggc
    16021 tgatgctggg caatggcata acgctggcta tcaaagccgc catcattaag ttgaccatga
    16081 actgcacgaa ctttgagttt cattgaccac agttgtccag cacaaaccgc ttgcggaaga
    16141 tattcgccat acagcacgag gcctggcgca gggaatatac gtcgaccttg tagatgagta
    16201 atttgaccgt aatgagtggt cataccgtca gtagcagtga tctcaacaat tgcatcctgc
    16261 gtcgcgccag ttaaggtttc tcctgcccac aaaatttgct ttgctgctaa tatgccccaa
    16321 acgaaaaaca gcaacgtcag cgcgatataa cggacagttt taaaagggat aaataccagt
    16381 acgcaggcga agacggtcag agacgcaagg gttaatgtcc caggcaattg gggcaaaatc
    16441 agcaacggaa aaattccgca aattatgcat acgccgactg tcgttatttt catgcacccc
    16501 tccctgttta tggaaagtgt gcaactttgt tgagcggttg tctggtggga gttttgcgtt
    16561 ccccgaggca cgttcagaag aatttaacga gagggtgaaa actgttcaaa actctgcgag
    16621 gctgcttcca gttccagatt agagaaatga aaaaagcacc cgacaggtgc ttttctcgcg
    16681 ttcaagtttg agtaaaaact taaccgtaaa tattggcgcg atcgcgcagt tctttacctg
    16741 gtttaaagtg aggaacgtat tttccttcca gttctacttt atcgccagtc ttcggattac
    16801 gtccggtacg tggtgcgcgg tagtgcaaag agaaactgcc gaaaccgcgg atttcaatac
    16861 gctcgccctg cgcaagagtc gaggccatat gctccagcat ctcttttact gcatcttcaa
    16921 ccgtcttggc gggaatgtgc gattgctggg tggcaagtct ttctatcaat tctgacttgg
    16981 tcatgattcc tccggttcct taaaggcaaa tttagcggct gctgttgctg cattgattaa
    17041 gggcggccgt agccgccctt agtgcttgat tacaggacga aacctgcaat ctgtcaagta
    17101 aactcaacaa acttcggaat aaaaatcccg aagagtcaga gaattactcg cctttagctg
    17161 ctttgaaagc ttcagccatt gcgttgttgg agaagtttgc atcttcctgt ttgttaacag
    17221 ttgcgattgc atctttctcg tcagcttcgt ctttcgcacg aacagacagg ctgattgcgc
    17281 ggtttttacg atcaacgccg gtgaatttag cttcaacttc gtcgccaacg ctcagaacca
    17341 gggtagcgtc ttcaacgcgg tcacgggatg cttcagaagc acgcaggtaa ccttcaacgc
    17401 cgtcagccag ttctacggtt gcgcctttag cgtcaactgc agttacttta ccggttacga
    17461 tagcgccttt cttgttcaga gcaacccagt tgttgaacgg atcttctgcg agctgtttaa
    17521 cgcccaggga gatacgttca cgttctgcgt caacctgcag aacaactgca gcgatttcgt
    17581 cgcctttttt gtattcacga actgcttctt cgcctgcaac gttccaggag atgtcagaca
    17641 ggtgaaccag gccgtcgatg ccgccgtcca ggccgatgaa gataccgaag tcagtgatag
    17701 acttgatttt accttcaaca cggtcgccct tgttgtgggt ttccgcgaac tgctgccacg
    17761 ggttagcttt gcactgtttc agacccaggg agatacgacg acgttcttcg tcgatatcca
    17821 gaaccataac ttccactaca tcgccaacgt taacaacttt ggacgggtgg atgtttttgt
    17881 tggtccaatc catttcggaa acgtgtacca ggccttcaac gccttcttcg atttcaacga
    17941 agcagccgta gtcggtcagg ttggtcacgc gaccagtcag tttggtacct tccggataac
    18001 gtttagcgat agctacccac ggatcttcgc ccagctgttt caggcccagg gatacacggg
    18061 tacgttcgcg gtcgaacttc agcactttaa cagtgatttc gtcgcccacg ttgacgattt
    18121 cgctcggatg cttaacgcgt ttccaggcca tgtcagtgat gtgcagcagg ccgtcaacgc
    18181 cgcccagatc aacgaatgca ccgtagtcag tgaggttctt aacgatacct ttaacttcca
    18241 tgccttcctg caggttttcc agcagctgat cgcgctctgc gctgttttcg gattcgataa
    18301 cggcacgacg agaaacaaca acgttgttgc gcttctgatc cagcttgatt actttgaatt
    18361 caagctcttt gccttccagg tgcagagtgt cacgcaccgg acgaacgtct accagagaac
    18421 ctggcaggaa cgcacgaata ccgttcagct caacagtgaa gccgccctta actttgccgt
    18481 tgataacacc ggtaacagtt tcagcatctt cgtaagcttt ttccagcgtg atccaggctt
    18541 cgtgacgttt agctttctca cgggacagca gagtttcacc gaagccgtct tctactgcgt
    18601 ccagagcaac gtcaacttcg tcacctacct ggatttccag ctcgccctgg gcgtttttga
    18661 actgctcagc cgggatggcg gactcagatt tcagaccagc gtcaaccagt actacgtctt
    18721 tgtcgatagc aacaacaacg ccacgaacga tagaacccgg gcgggtttcg atttctttta
    18781 aggactcttc aaagagttga gcaaaagatt cagtcatgtt taatcttcag gtttatattt
    18841 aacgtccacc tggctccatg ccggatgggg ttgtttaaca tacccgctgt cattccattg
    18901 caacgggggt actgcaaatt cggtcgctta tgcgagagcc aatttctggc gcgcgtattg
    18961 tagcgctttt tcaatcactt gctcaatgct taaggtggtg gaatccaaca ctaaagcatc
    19021 ggctgccgga accagtggcg ctaccgctcg gttacgatcg cggtcgtcgc gttctttgat
    19081 ctcagccaaa aggcgctcaa agttaacact aaagcccttc tcctgcaact gtagcatgcg
    19141 gcgatgcgca cgttcttccg aggaggcgtc aaggaaaatt ttcactggcg catcagggaa
    19201 taccaccgtt cccatgtcac ggccatcggc aatcaggcct ggtaattcgc gaaacgcgcg
    19261 ttggcgacgc aataatgctt cacgaacgcg tgggaatgcc gcgacttgtg aagctgcatt
    19321 cgccacttcc tgagtacgaa tttcgccact gacatcttcc ccttcgagga tcacttccag
    19381 attgccattg gtcgacacaa aacgtacatc cagatgggat gccagcggca ccagcgcatc
    19441 ttccgatgca acatcaacat ggtgatgtaa tgccgccagt gccagtacgc gataaattgc
    19501 acccgagtcc agcagatgcc attgcaacgc ttccgccata gccttacaca aggtgccttt
    19561 ccctgcaccg cttgggccat caatggtaat aaccggggca attgccgtca tctttatctc
    19621 cttaaaacag gcgtaccgtt aacataaccg cgcgcattat acgcgccgac gccacaaatc
    19681 gttaacgttg tgtgcaaaaa ccagttttgt ctgtattcag atggggattt attgcgcaat
    19741 tatagcgggc tggcagaaga ccagcccgaa agatgacaat gatttactta ccagaggcga
    19801 tacgatcacg gatgtgttgc gcacgctctg ttgatggtgg gtgagaatca aacatggact
    19861 gggtacgacc gccatccagg ctggccaatt tttcaaagct gccaaccagc ccctgggtgc
    19921 tgatgccacg tttcttcaac agatcaaagg agaaatcatc tgcttcggac tctttatcgc
    19981 gggagtattt agcattgatc gcgccttctg cgatatcacc taattgtgag cgagaaagct
    20041 gggaagccac accgctggta gcggaaatcg catcacgtgc ggcaacgatc gcataagaag
    20101 ctttcatttc agccagcgag tgacccaacg cgacgtggcc cagttcatga cccagaacgc
    20161 cttcaatttc gttgtcgttc atcatgtcca tcaggccact gtagacacga acacaaccgt
    20221 tcgccatcgc ccatgcgttg acgtcgctgg tcatataaac cttatagttg accggcgtgc
    20281 cattaatatt gttacccaat gctttggcga tttttgccag acgtttcccg tacttgctgg
    20341 atttgcttgc gacttgattg ccgctgtcca tttcggcaca gccctgattg gcaatcgctt
    20401 ttgcatcagc atcgcttaac gttgctgctt tataggcatt taaacctgag ctgatagcca
    20461 tattggtatc aataccgtgg gtattttgac accctgtcag taatgctgca gaggccgcaa
    20521 tcgccagcag taatttagta ttcttcatgt gaatcatccg ttgattaatc atatataaat
    20581 attatgtatc aataacttac attgacaagc gacgctacca ttaatcaaaa tgactcacaa
    20641 ggtccgaaaa aaaacgccgg caatgtgccg acgttttgat aaagaaagat ttggctattt
    20701 attgcacgtt gttcattcag gctgcctggc taatccgcgc cagctgctcg aaataattcg
    20761 gaaatgtttt ggccgtgcat ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca
    20821 ccagcgagaa acacatcgct atccggtgat cattgtatgt cgcgatctcg gcaaagttca
    20881 gcttttccgg tggagtgata cgaatgtaat cgtgcccctc ttctacttcc gcaccaactt
    20941 tacgcagttc tgttgccatc gcaaacaggc gatcggtctc tttaacacgc cagttataga
    21001 tattgcgcag cgtggtggtg ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg
    21061 catcgggaat atggttcata tccatatcaa tagcgttcag ttcaccccgc gtgcaggaaa
    21121 tataatcatc gccccagcaa atggtcgcgc ccattttttc cagcacatca gcaaagcgaa
    21181 tatcaccctg catactgttg cgtccaatgc cggtcacttt tacagtgccg cctttgattg
    21241 ctgctgctgc caggaagtaa gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg
    21301 gagactgata agactgcccg ccttttacga caaattgttg atagtgctga ttttcaattt
    21361 caacaccaaa cgtcttcatc atattgagtg tgatgtcgat ataaggttta gaaaccagat
    21421 cgcctttaat acgaatcacc gtatcttccg gtgcaagagg cgcagtcatt aatagtgcgg
    21481 tgaggaactg gctggaaacg gagccatcaa cgtcaacgtt gccgccagta aagccgccct
    21541 gtaaacgcaa tggcggataa ttgtcttgtt ccaggtaagt gatcttcgcc ccgcccaggc
    21601 gcagcgcatc caccagatga ccaatcgggc gctctttcat acgcggctca ccagtcagca
    21661 caatatcatt gctacccaga caaagagctg ccgccagcgg gcgcattgcc gttccggcgt
    21721 ttccgaggaa caactccagg gcaccttctg cgtgtaatgg accgccgtta ccgataattt
    21781 cgcaacgcgt acgatcggct gaaagcgtat agcttacccc taacgctgtt aatgcattca
    21841 gcatatggcg cacgtcatcg ctatccagca gattggttaa tactgttttg ccgtgtgcta
    21901 atgccgccag caataaagcg cggttagaaa cgctcttgga accaggcaga ttaatagtgc
    21961 catcgacacg agcgatgggt tgtaacgtca gggattccat gaactcaact ctctacaaca
    22021 gaaataaaaa ccccacaggc tggctgtggg gattaagcaa aatttcggca ttagccgtga
    22081 cggcgttcga actcaaccat gaagtctgtc agggctttaa cgccttccag cggcatggcg
    22141 ttataaatag acgcgcgcat tccgccgacc acacggtgac ctttcagcgc atgaaggcca
    22201 gcagcaaaag actcttcaag gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc
    22261 acgttcatcc gcgaacggtt agctttcgcc acgtcattgc ggtagaaatt gctgttatca
    22321 atcaccccat acagcagttc tgctttttgc tgattgattt tatccattgc agctacaccg
    22381 ccgttcgctt tcagccattt aaagaccaga ccagacagat accaggcaaa tgtcggcggc
    22441 gtgttaaaca tggagtcgtt atcgttgaga atggaataat ccagaatcga cggacacgcg
    22501 atattcgctt tgcccagcaa atcttcacga acgatgacga ttgtcaggcc agccgggccg
    22561 atatttttct gcgcgccagc gtaaatcacg ccataacggc tgacgtcaat cggacgggaa
    22621 agaatggttg aagagaagtc ggcggcgacc accacatctt tgccgaagtc tggcgtttcg
    22681 tcgatagcga taccgtcgat ggtttcattc gggcagtaat gcatataagc agcattatca
    22741 gagagttgcc attcgcgcat tggcttaacc gcgcgcagac catcaacagt cactttggcg
    22801 tcaaagacat taggcgtgca gtattttttc gcttctttaa tggcacttgc cgcccagtaa
    22861 ccggcatcaa cataatctgc ggtggtttta tcaccgagaa tattcagcgg aacagcagca
    22921 aactgaccgc gaccgccgcc atggcagaac aataccttgt agttggaggg gacattaaga
    22981 agatcgcgaa aatccttctc ggcttcctct gcaacctgaa tgaactcttt gccacggtga
    23041 ctcacttcca tcaccgacgt accaagaccg ttccagtcgc gcagttcctg ttgagcctgt
    23101 ttaagcacct ctgccggtag cattgccgga ccagaactaa aattgaagat ttgagccatt
    23161 tcccctcacc acgttgcgtt gctattaacc cgcggtcaat cgattgcgac cacaggtaat
    23221 acattattcc tgtggctatc ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac
    23281 cgttgagggc aaaaatgtgg cttgcatcac acaaaagaat ctctcggatt attagcaaaa
    23341 agccgatgtt tttactgtca cgtcacccgt tttgcccgac ttgccttcgt ccattctgga
    23401 tttgcacagc gcggacataa ttgtttttcc cccgcgttca tatgaatgat ttcactgcaa
    23461 cgaatgcagg catagtccac cgactcgggt accactttgt aacgctgagt acagtcactg
    23521 ggtaaaataa gtaagcccgg tttcaccttg tcatcttcaa agaaatagac actgatttgc
    23581 ccgttggttt cgagaatcgc cagacgtact tgccccaact gctctacacc gcgcaatcgt
    23641 agctccataa agaactcaaa ttccgtcatg ttggagttat tgagtttcga ccaggccagc
    23701 tcgccatctt caataatgac aaccggcttg ccttccagaa gatcttccag tttttcactg
    23761 tgcgccatca accacattac caggcggtat aacaacgcca gaataataaa gacgataagt
    23821 accgggacca tcggcacatc atcataaaac gccacatctc ccgccgctga tcccagcgtc
    23881 agaatgatta acacttcaaa aagcgacatc tgccgcacac cgcgtcttcc ggtcattttg
    23941 aggaacaaaa aaaccagtac aaaggtataa agactacgta gtgccacttc gccaaggaaa
    24001 tcaaaaggca ctttatcaaa tgccatacgg tggagatcga acgccttcat ttatatgctc
    24061 ttatcgtggt ttatccgcta aaagcatagt caacagatca tcggcatggc ggcggtgata
    24121 gcgcctttga gataaaaccc gtattattgc gcgctttccg tacgactaaa gtgattttca
    24181 tgacgcaaac atttatcccc ggcaaagatg ccgcactgga agattccatc gctcgcttcc
    24241 agcaaaaact ttcagacctc ggctttcaga ttgaagaggc ctcctggctg aatccggtgc
    24301 ctaacgtctg gtctgtacat attcgcgaca aagagtgcgc actgtgtttt accaacggta
    24361 aaggcgcaac caaaaaagcg gcgctggctt ctgcactcgg tgaatatttc gagcgtctct
    24421 caaccaacta cttttttgct gatttctggc tgggcgaaac catcgccaac ggtccgttcg
    24481 tgcattatcc caacgaaaaa tggttcccac tgagcgaaaa tgacgatgtg ccagaagggc
    24541 tgctcgatga ccgtctgcgc gcattttacg atccggagaa tgaactggcc ggcagtatgc
    24601 tgattgacct gcaatccggt aacgaagatc gtggtatttg cggtctgccg tttacgcgtc
    24661 agtctgataa tcagaccgtt tatattccga tgaatatcat tggtaacctg tatgtttcta
    24721 acggtatgtc cgctggcaat acccgtaacg aagcacgcgt tcaggggttg tccgaagttt
    24781 tcgaacgcta cgtgaaaaac cgcattattg ctgaaagcat cagcttgccg gaaatcccgg
    24841 cagacgtgct ggcgcgttac ccagcggtgg ttgaagccat cgaaacgctg gaagcagaag
    24901 gtttcccaat ctttgcttat gatggttcgc ttggcggcca gtatccggtg atttgcgtgg
    24961 tactgttcaa tcctgctaac ggtacctgct ttgcctcttt cggtgcgcat cctgattttg
    25021 gcgtagcact ggaacgtacc gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg
    25081 atgtgtttac tccgccaacc ttcgatgatg aagaagttgc tgaacatacc aacctcgaaa
    25141 cgcactttat cgattccagc ggtttaatct cctgggacct gttcaagcag gatgccgatt
    25201 atccgtttgt ggactggaat ttctccggca ctacggaaga agagtttgct acgctgatgg
    25261 ctatcttcaa caaagaagat aaagaagttt atattgccga ttacgagcat ctgggcgttt
    25321 atgcttgccg aattatcgtg cctggcatgt ccgatattta tccggctgaa gatctgtggc
    25381 tggcgaataa cagtatgggc agccatttac gtgaaacgat tctttcgtta ccaggcagcg
    25441 agtgggaaaa agaagattac ctgaacctca tcgagcaact ggatgaagaa ggttttgatg
    25501 actttacccg cgtgcgtgag ctgttgggtc tggcgaccgg gtcggataac ggttggtaca
    25561 ccctgcgtat cggtgaatta aaagccatgc tggcgctggc tggtggcgat ctggaacagg
    25621 ctctggtctg gaccgaatgg acgatggagt ttaactcatc ggtattcagc ccggaacgcg
    25681 ccaactatta tcgctgcctg caaacgttgt tattactggc gcaggaagaa gatcgccagc
    25741 cgctgcaata tctgaatgcg tttgttcgca tgtacggcgc agatgccgtg gaagccgcca
    25801 gtgcggcaat gagcggcgaa gcggcgtttt atggtttgca accagtagat agcgatctgc
    25861 acgcgtttgc tgcacatcag tcgttgttga aggcctacga aaagctgcag cgcgccaaag
    25921 cagcattctg ggcaaaataa aatcaaatag cctacgcaat gtaggcttaa tgattcgtct
    25981 gagttatatt acggggcgtt tttttaatgc ccc
//
LOCUS       DAZGHF010000069        25532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGHF010000069 DAZGHF010000000
VERSION     DAZGHF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.069"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(29..1678)
                     /gene="pgi"
                     /locus_tag="ACHK3A_002869"
     CDS             complement(29..1678)
                     /gene="pgi"
                     /locus_tag="ACHK3A_002869"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO8740925.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      1915..2111
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2203..3552
                     /gene="lysC"
                     /locus_tag="ACHK3A_002870"
     CDS             2203..3552
                     /gene="lysC"
                     /locus_tag="ACHK3A_002870"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO8740926.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3805..4077
                     /locus_tag="ACHK3A_002871"
     CDS             3805..4077
                     /locus_tag="ACHK3A_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO8740927.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4078..4950)
                     /gene="rluF"
                     /locus_tag="ACHK3A_002872"
     CDS             complement(4078..4950)
                     /gene="rluF"
                     /locus_tag="ACHK3A_002872"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO8740928.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5298..6245
                     /locus_tag="ACHK3A_002873"
     CDS             5298..6245
                     /locus_tag="ACHK3A_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO8740929.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVNALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6315..7121
                     /locus_tag="ACHK3A_002874"
     CDS             6315..7121
                     /locus_tag="ACHK3A_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8740930.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTRLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVTDFVCYLLSQRASYITGVTTNIAGGKTRG"
     gene            7131..7538
                     /locus_tag="ACHK3A_002875"
     CDS             7131..7538
                     /locus_tag="ACHK3A_002875"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO8740931.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     HSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7538..8032
                     /locus_tag="ACHK3A_002876"
     CDS             7538..8032
                     /locus_tag="ACHK3A_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO8740932.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQHIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8098..8895
                     /locus_tag="ACHK3A_002877"
     CDS             8098..8895
                     /locus_tag="ACHK3A_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO8740933.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            8906..9730
                     /locus_tag="ACHK3A_002878"
     CDS             8906..9730
                     /locus_tag="ACHK3A_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO8740934.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLLVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9782..11011
                     /gene="sorE"
                     /locus_tag="ACHK3A_002879"
     CDS             9782..11011
                     /gene="sorE"
                     /locus_tag="ACHK3A_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO8740935.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAIGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11393..12082
                     /gene="pepE"
                     /locus_tag="ACHK3A_002880"
     CDS             11393..12082
                     /gene="pepE"
                     /locus_tag="ACHK3A_002880"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO8740936.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12172..13803)
                     /locus_tag="ACHK3A_002881"
     CDS             complement(12172..13803)
                     /locus_tag="ACHK3A_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO8740937.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14023..17706)
                     /gene="metH"
                     /locus_tag="ACHK3A_002882"
     CDS             complement(14023..17706)
                     /gene="metH"
                     /locus_tag="ACHK3A_002882"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO8740938.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMEFLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLSGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            17906..18730
                     /gene="iclR"
                     /locus_tag="ACHK3A_002883"
     CDS             17906..18730
                     /gene="iclR"
                     /locus_tag="ACHK3A_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO8740939.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            complement(18747..20471)
                     /gene="aceK"
                     /locus_tag="ACHK3A_002884"
     CDS             complement(18747..20471)
                     /gene="aceK"
                     /locus_tag="ACHK3A_002884"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO8740940.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGNLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRK
                     QRFSVRFV"
     gene            complement(20687..21991)
                     /gene="aceA"
                     /locus_tag="ACHK3A_002885"
     CDS             complement(20687..21991)
                     /gene="aceA"
                     /locus_tag="ACHK3A_002885"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO8740941.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(22021..23622)
                     /gene="aceB"
                     /locus_tag="ACHK3A_002886"
     CDS             complement(22021..23622)
                     /gene="aceB"
                     /locus_tag="ACHK3A_002886"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO8740942.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKHILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(23891..24820)
                     /gene="metA"
                     /locus_tag="ACHK3A_002887"
     CDS             complement(23891..24820)
                     /gene="metA"
                     /locus_tag="ACHK3A_002887"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO8740943.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            24977..25420
                     /locus_tag="ACHK3A_002888"
     CDS             24977..25420
                     /locus_tag="ACHK3A_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO8740944.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAAYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         6022 a         6672 c         6692 g         6146 t
ORIGIN      
        1 ccttatccgg cctacatatc gacaatgatt aaccgcgcca cgctttatag cggttaatca
       61 gaccattggt cgagctatcg tggctgctga tttctttatc atctttcagc tctggcagaa
      121 tacggttcgc cagctgttta cccagctcca cgccccactg gtcgaaggtg aagatgttca
      181 gaatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaatgca cccaggctga
      241 acggagtgat ttcacgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
      301 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcacgatat tcctgctcaa
      361 ccacttcgcg ggatttacca aacgccagcg cttcggtctg agcaaagaag ttagacagca
      421 gtttctggtg atgatcagag agcgggttat gggtgatagc cggagcgatg aaatcgcacg
      481 gtaccatttt ggttccctgg tggatcagct gatagaacgc gtgctgaccg ttcgtgccag
      541 gttcacccca gataatcggg ccagtctggt aatccacaac cttaccgtta cggtcaacat
      601 acttaccgtt ggattccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
      661 ggtcatacgg cagaatcgct tcagtttccg cgccaaagaa attgttgtac cagatgccaa
      721 tcagcgccag caagacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
      781 tcgcgtgtgc gccagaaagc agttcaacaa aattatcaaa gccgatggag agaacaatcg
      841 acaggccaat tgctgaccac aaagagtaac ggccgccaac ccagtcccag aactcgaaca
      901 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
      961 agtgtttcgc aacgtgtttt tcatcaccag ccgctttcag gaaccagtca cgcgcgctgt
     1021 gggcgttggt catggtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
     1081 tttccgggtt tacttttttc agcacttccg cgatgtgagt cccatcgacg ttagaaacaa
     1141 agtgcatgtt caggtggttt ttgtatggac gcagtgcttc agtcaccatg tacgggccga
     1201 ggtcagaacc gccgatcccg atgttcacta cgtcagtgat tgctttgccg gtatagcctt
     1261 tccactcacc ggaaataatc gcttccgaga aggttttcat cttctccagc accgcgttga
     1321 cttctggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
     1381 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
     1441 taatcgcgcc cgccagatcg cactctttcg ccagatcttg taatttcgcc agcgtctctt
     1501 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
     1561 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
     1621 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
     1681 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
     1741 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagcgaa
     1801 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccatc
     1861 gttgacaacg gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
     1921 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
     1981 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
     2041 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
     2101 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
     2161 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
     2221 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
     2281 gtgctttctg atgccaacgt gcgtttagtt gtcctctcgg cttctgctgg tatcactaat
     2341 ctgctggtcg ctttagctga aggactggaa cctggcgagc gattcgaaaa actcgacgct
     2401 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
     2461 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
     2521 tctccggcgc tgacagatga actggtcagc cacggcgagc tgatgtcgac cctgctgttt
     2581 gtcgagatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
     2641 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
     2701 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
     2761 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
     2821 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
     2881 tacaccaccg atccacgcgt ggtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
     2941 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
     3001 gcagtacgca gcgatatccc agtctttgtc ggctccagca aagacccacg cgcaggtggt
     3061 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
     3121 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
     3181 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
     3241 gtgagtgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
     3301 acgcaatccc tgctgatgga gctttccgca ctgtgccggg tggaagtgga agagggtctg
     3361 gcactggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
     3421 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
     3481 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtag tgcagaagct gcattttaat
     3541 ttgtttgagt aaacacagta tggcccggaa gacattattt cgggccgtat tgtttttctt
     3601 gtcattatgc ccataaataa acgagcctgt actctgttaa ccagcgtctt tatcggagaa
     3661 taattgcctt taatttttta tctctttctc taactaatta gagaaagaga taaatagtta
     3721 agagaaggca aaatgaatat tatcagttct gctcgcaaac gaattcccgt acactgaact
     3781 ctctggctca ctgagggcac catcatggca ctcccccgta ttacccaaaa agagatgact
     3841 gaacgcgagc agcgtgaact taaaacactg ctggatcggg cgcggattgc gcatggtcgc
     3901 gtactgacca actccgaaac caacagcatt aagaaagagt acatcgataa attaatggtc
     3961 gagcgtgagg ctgaagcgaa aaaagcccgc caattgaaga aaaagcaggc ttataaaccg
     4021 gatcctgagg cgtcatttag ctggtcagca aatacgtcga cgcgcggcag gcgctgatta
     4081 acgccccttc tttttgcgcc ccggcgaggt aaaacgctta ccgttggacg ccgggcgacc
     4141 gcctttttcc gccgtttttt ccatcttaac gactggacgt ttgatgccta ccgttttcgg
     4201 tttggccttc gctttaggtt ttgcctcgga agaggaattt tcgatgagct taaagaggtc
     4261 gattaactca tcgtcggtta aatcgcgcca ctcccccagc ggaatgccgc ttaaactgac
     4321 gttcataatg cgcgtacgtt ccagcttttt cacttcgtaa ccaaagtgct cgcacatgcg
     4381 acggatctga cggttcagcc cctgcaccag ggtaatgcgg aagacaaatg gcgcttcttt
     4441 tttgactttg cactttttag tcactgtccc gaggattggc acccccgccc ccataccacg
     4501 aataaattca tcggtaatcg gtttatcgac cgtcaccaga tactctttct catgatcgtt
     4561 acccgcacgg aggatcttat tcaccagatc gccgtgattg gtgaggaaaa tcagcccctg
     4621 ggagtcttta tccaggcggc caatcgggaa tacgcgtttg ctgtggttaa cgaaatcgac
     4681 aatgttatcg cgctcgccat cttcggtggt gctgacaata ccgacgggct tgttcagggc
     4741 gataagtacc aaatcttcgg cttcccgagg ttcaatcaac tgaccattta ctttcacaac
     4801 gtcgccgggt ttcacctgat cgccaatggt ggctcgcttg ccattaagga acacattgcc
     4861 ttgctcgata tagcgatccg cttcgcggcg tgagcaaatt ccgctttcgc tgatgtattt
     4921 atttaaacgg actgatgagt cgggcagcat aaattctcct gtaaaacgcg gaatataccg
     4981 tacctcttag cggggaaaaa cagggttatg cggcaacgtc gaaattaaat gtgatctgcc
     5041 acgcctttta tcacaaaagt gtctccggaa gtacgatgca aatttgcctg gcaaaataag
     5101 gataatcaac atattacgaa tcgctgcaca cctgtgctga tgactactac gcagagaaac
     5161 aaatgtgcaa gagaaagcgc tatttctccc catgcaaaga gtgaaaaatc gctttgaaag
     5221 gccctcatga tggggttata tcagatcaag cggtgagaaa gcgtatttgc acaaatgtgc
     5281 agaaccagga ggcggcaatg gaaaacagtg acgatatccg tttgattgtg aagattgccc
     5341 aactctatta cgaacaggat atgacgcagg cgcaaatcgc gcgcgaactg ggtatttacc
     5401 gcaccaccat cagccgcttg cttaaacgag gccgcgatca gggaattgtc accatcgcca
     5461 tcaactatga ctacaacgaa aatctctggc tggagcagca actgaagcaa aagtttggcc
     5521 tgaaagacgt tgtggtggtg tcaggaaatg atgaggatga agagactcaa ctggcgatga
     5581 tggggttaca cggcgcgcaa ctgctggatc gcttgctgga acctggcgat attgtcggtt
     5641 tttcctgggg ccgcgcggtg aacgcactgg ttgaaaactt gccgcaggcg gggcaatcgc
     5701 ggcagttaat ctgcgtacca attattggcg gaccgtccgg taaactcgaa agccgctatc
     5761 atgtaaacac attaacctac agcgcggcag cgaagttgaa aggggaatcg catctcgcgg
     5821 attttccggc tctgctggat aacccattaa ttcgtaatgg gatcatgcag tctcagcact
     5881 ttaaaaccat ctctgcctac tgggataatc tggatgtcgc cctggtggga attggctcac
     5941 cggccattcg cgacggcgct aactggcatg cgttttatgg tggtgaagag agtgacgacc
     6001 tgaatgcccg ccaggttgct ggcgatattt gctcgcgctt ttttgatatt cacggcgcaa
     6061 tggttgaaac aaatatgagc gaaaaaacac tctctatcga aatgaataaa ttaaagcagg
     6121 cgcggtattc cattggcatt gccatgagtg aagaaaaata cagcggaatt attggtgcac
     6181 tgcgtggaaa atatattaat tgtctggtaa cgaatagcag cacagctgaa ctattactga
     6241 aataaaaatc ttcccgatgt ttaccgtaat aaaaattacg gcgggtctct attatctaaa
     6301 atcaggagtc tgttatgcaa acgtggttaa atttgcagga taaaatcatt attgtcaccg
     6361 gtggcgcatc cggcattggt ctggcgattg tggatgaatt attagcacaa ggcgcgaatg
     6421 tacagatggt cgatattcac ggtggcgatg gacaatatga aggtcataaa ggctatcagt
     6481 tttggccgac cgatatttcc agcgccaaag aggtaaatca tacggtagcg gaaattatcc
     6541 agcgttttgg tcgcatcgac ggtctggtca ataacgccgg ggtcaatttc ccgcgtctgc
     6601 tggtcgatga gaaagcgcct gccgggcagt atgaactcaa cgaagctgca ttcgaaaaaa
     6661 tggtcaatat caaccagaaa ggcgtttttc tgatgtcgca ggcggtggcg cgacagatgg
     6721 tcaaacaaca tgatggcgtg attgtgaatg tttcctcaga aagtgggctg gaaggctcag
     6781 aaggccaaag ctgttacgcc gctaccaaag ccgcgcttaa tagcttcacg cgatcctggt
     6841 cgaaagaatt gggtaaacac ggtatccgtg tagtcggtat cgcgccgggg attctggaaa
     6901 aaaccaggct acgcacgccg gaatatgaag aggcgctggc gtggacgcgc aatatcaccg
     6961 tcgagcagct gcgtgaaggc tataccaaaa acgccattcc tattgggcgc gccggaagat
     7021 tagcagaagt cactgatttt gtttgttatc tgctgtctca acgcgccagc tatatcaccg
     7081 gagtaaccac taacattgcg ggcggcaaaa cgcgcggcta aggaggcttt atggtcaacg
     7141 ccattttttg tgcccacggc aaattagctt gcgccatgct ggaatcggtt cagatggttt
     7201 acggcaacgc caatgttgaa gccgttgaat ttgtgcccgg tgaaaacgcc ggagacatcg
     7261 tagcaaagct ggaaaagtta gtaagcattc acaatcaaga tgagtggctg attgccgtgg
     7321 atttacagtg cggcagcccg tggaatgcgg cagcgatgct tgcgatgcgc aatcctcgtc
     7381 tgcgggtaat cagcggcctt tctctgccgc tggcgctcga actggttgat aaccagcaca
     7441 gcatgaatgt tgatgaactg tgtgaacacc tgacgcaaat cgcaaagcaa acctgcgttg
     7501 tctggaaaca actggcaacg gcagaggagg atttctgatg aacattacgc tcgcccgcat
     7561 tgatgaccgt ctgatccacg gccaggtcac taccgtctgg tcgaaagtgg ctaatgccca
     7621 gcacattatt atctgcaatg acgaagttta taacgatgaa gtgcgccgga cattattgcg
     7681 tcaggctgcg ccaccgggaa tgaaggttaa cgtggtcaat attgaaaaag ccgtcgcggt
     7741 ttatcacaac ccgcaatatc aggacgaaac cgttttttat cttttcactc gcccacagga
     7801 tgccttagcg atggttcgcc agggggtaaa aatagacacc ctgaatattg gcggcatggc
     7861 ctggcggccc ggtaaaaaac agttaaccaa agcggtttca ttagatgatg atgatattaa
     7921 cgcatttcat gagttaaata atcttggcgt catactggat ttacgcgtcg tagcgtcaga
     7981 tccctcaatc aatattatcg acaaaataaa cgaacagtta atcgcaaatt aaataatagc
     8041 gcctgtgtac atatgcttta acaggcaggg atgacgacac ttaaaaggtg acaaatcatg
     8101 gaaataagta ccctacaaat cattgctata tttctttttt cctgtattgc cggaatgggc
     8161 agcgtgctgg atgaatttca gactcatcgt ccgttaattg cctgtacggt gattggttta
     8221 attctcggtg atttaaaaac cggaattatg ctcggtggta cgctggaatt gatagctctc
     8281 ggctggatga acgtcggcgc ggcgcaatct ccggattctg cactcgccag cataatctcc
     8341 gccattctgg ttatcgttgg tcagcaaagc atcgccaccg gaatcgccat cgcgttgcct
     8401 gtggctgcgg caggccaggt gctgacggtg tttgcccgta ccatcaccgt agtgttccag
     8461 cacgcggcgg ataaagcagc ggaagaagca cggtttcgca ctctcgatat tctgcatgtc
     8521 tccgcgcttg gcgtgcaggc actgcgcgtt gctattccgg cactgattgt ctcgctgttc
     8581 gtcagcgctg atatggtgag caatatgctg agcgccattc ccgaatttgt gacccgtgga
     8641 ctgcagattg ctggcggttt tatcgtggtg gtcggttacg ccatggtgct tcgcatgatg
     8701 ggcgtgaaat atttgatgcc tttctttttc ctcggtttcc tcgcaggtgg ctacctcgat
     8761 ctcagtctgc tggcgttcgg tggcgtcggc gtgatcatgg ccctgctcta catccagcta
     8821 aatccacagt ggcgtaaagc tgaacctcaa ctccaggcca ccgcctccac cgcccttgac
     8881 caacttgacg attaacggag ccatcatgga acagagaaaa attacacgca gcgatctggt
     8941 gagcatgttt ctgcgctcca acctgcaaca ggcgtccttt aactttgaac gtattcacgg
     9001 gctgggtttt tgctacgaca tgatccccgc catcaagcga ctttacccat taaaagagga
     9061 tcaggttgcg gcgctcaggc gacacctggt gttcttcaat accacgccag ccgtatgtgg
     9121 cccggtcatc ggcgtcactg ccgccatgga agaggcgcgg gccaacggcg cggaaattga
     9181 tgacggtacc attaacggca tcaaagtcgg tctgatggga ccattggcag gagttggcga
     9241 tccactggtc tggggaacgc tgcgcccgat taccgccgcg ctcggcgcat ctctggcact
     9301 ttcgggcaac attctcggcc cgctgctgtt tttctttatt ttcaatgcgg tgcgtctggc
     9361 gatgaagtgg tatggcctac agctcggctt tcgcaaaggg gtgaatatcg tcagcgatat
     9421 gggcgggaat gtgctgcaaa aactcaccga aggcgcgtcg attctcggac tgtttgtgat
     9481 gggcgtgctg gtgaccaaat ggacgtcaat caacgtaccg ttgctggttt cacaaacgca
     9541 tgccgccgat ggctccaccg tcaccatgac cgtgcagaac attctcgacc aactttgccc
     9601 tggtttgctg gcgctcggtc tgacgctgct gatggttcgt ctgctcaaca aaaaaattaa
     9661 cccggtatgg ctgattttcg ccctgtttgg tttagggatt atcggtaatg cgctgggctt
     9721 cctgtcctga ttcttcgccc cggcacgact gccggggtca tcgctcaaca tgaggtggtt
     9781 tatgaaaaca acagctctgc gtctttatgg caaacgtgac ctgcgtctgg aaacctttga
     9841 ccttcctgaa atgcaggagg atgaaatcct cgcaacggtg gtcactgaca gcctgtgcct
     9901 ctcttcctgg aaagaggcca atctgggtga aaaccataaa aaagtacccg acgatgtggc
     9961 gaccaacccc atcatcatcg gccacgagtt ttgcggcgat attctggccg tgggtaaaaa
    10021 gtggcagcac aaattccagc cgggtcagcg ttatgtgatt caggccaacc tgcaactccc
    10081 cgaccgcccg gattgccccg gctactcctt cccgtgggta ggcggcgaag cgacgcatgt
    10141 ggttattccc aacgaggtca tggaacaaga ttgcctgctg gcatacgacg gcgaaaccta
    10201 ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt ggcgcgttca acgccaacta
    10261 tcatcttcag gaaggtagtt ataaccacac gatggggatt cgcccgcaag ggcgcacgct
    10321 gatcctcggc ggcaccggcc cgatggggct gctggcgatt gattatgcgt tgcatggacc
    10381 ggttaacccg tcgctgctcg tcattaccga taccgacaac gacaaattga gttatgcacg
    10441 caagcactat ccatcagaac cgcaaacact gattcattat ctcaatgccg ccgatgcagc
    10501 atttgatacg ttaatggcac tgagtggcgg tcacggcttc gatgatattt tcgtctttgt
    10561 gcctaatgaa ggactggtga ctctcgcctc ttccttgctg gcgacagatg gttgtctgaa
    10621 tttcttcgcc ggaccgcagg ataagcactt cagcgcgcca attaatttct acgatgtgca
    10681 ttatgcattt acccactacg tgggcacgtc aggcggcaat accgacgaca tgcgcgcagc
    10741 ggtcaaattg attgaagaga aaaaagtgca ggccgcaaaa gtggtaacac atattcttgg
    10801 gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc atcggcggcg gcaaaaagct
    10861 ggtgtatacc gggaaatacc tgccgcttat gtcactcacg cagattcagg atcaagcact
    10921 ggcggcaatt ctggcgcgtc atcaggggat ctggtccggt gaggcggagc aatacctgct
    10981 cgcccatgca gaggcaattt cccatgatta atcgcgctac ttaactctgc atgtcgctgg
    11041 ctggtcgccg cggcaacttc gcgacctgat gtgcggctga ttgctgaagc ggcacaatgt
    11101 gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg ctcaaattgg tggcaaagag
    11161 cacattgttt atgccggatg cggaatgaag ccttattcgg cctacataat cttgaaaatt
    11221 caatatattg cagttatctg gtaggccagg taagcgtagc gcatcaggca atcctgtgtt
    11281 tgtcatcagt ctcagcgcct gtgcaatatg tgacatcgtg agccatgtca gtaagtggtt
    11341 aaattcgcag tggtagcatt ccttccgtgt tttcttacta accggagaag taatggaact
    11401 gcttttattg agtaactcga cgctgccggg taaagcctgg ctggaacacg cgctgccgct
    11461 gattgctgaa cagttgcagg gtcgccgttc tgcggtattt attcctttcg cgggcgtaac
    11521 gcagacctgg gatgattaca cagcgaaaac ggctgcggtt ctcgctccgc tgggtgtttc
    11581 tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg cggaaattgt
    11641 gattgtcggc ggcggtaata ctttccagtt gctgaaacag tgccgcgagc gcggactgct
    11701 ggcaccaatt actgacgtgg ttaaacgtgg cgcgctgtat attggctgga gcgctggcgc
    11761 taaccttgct tgcccaacca ttcgtaccac caacgatatg ccgattgtcg atccacaagg
    11821 tttcgatgcg ctgaatctat tcccgttgca aatcaacccg cacttcacca acgcgctgcc
    11881 ggaaggccat aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc
    11941 agaactcaca attattggtc taccggaagg taactggatc acggtgagta aaggtcacgc
    12001 tacgctgggt ggcccgaaca ccacttatgt gtttaaggct ggtgaagaag cggttccgct
    12061 ggaagcgggt caccgttttt aagtgaattg ccggatgcat gatccggcat cttgtacata
    12121 gtgcctgatg cgacgcttta cacgtcttat caggccttca gggtcacggt attaatactc
    12181 gtcccgtcct tcgtcttcat ccggctgttc cagcacactg taagccaccg aacaaaacag
    12241 cgagttcaga cgctgcatat ctcccagtaa gcctaaatgt agcgaactgg tttcaatact
    12301 ctgcacgttt tgctgatgca ggcgatcgac gtgagcatgg gaataacggc gattaagaat
    12361 gcgaaagcga tgcttgctgc ggcgcaaacg acgggcgctg gtaacatcgc cagagaagaa
    12421 aaccgacatt gccagtttta aattactgag caattgctca tagagcgcat ccagttcctt
    12481 caagccatca agcgaaaatg cccgccgtgc tgccagcgat ttatcggcaa tttcactccc
    12541 catgcgctcg acgatatcgg aggcctgttc aaggttgagc gacatttcga tgatctccgc
    12601 ccagcggcgc gactcttctt ctgccagttc ctcttttggc atccgcgcca gatacagctt
    12661 aatggcggta tagagaacgt tgatgtcatc agccagctta cgcagctctt tctcctgccg
    12721 tggctcgccg tgcatcactt tattcaaccc ttccatcatc tgttccatgg cgtcgccaat
    12781 gcgcagggtt tcgcgggcag cgttcgccag agcaagcgtg ggcgtatcca gcgcgctgac
    12841 atccagatgt ttaggccgta gctgggtatc cagttccggt tcatcgcgaa taatcgtttt
    12901 gcaaaaccgt gccatcgggt caacaaatgg cagcatgacc aggcagcgta caaggttgta
    12961 gaagacgtgg aaatagatca ccaattccgc ttttggcaat ggcaacttcc ccattgtctc
    13021 tgccagcaaa tgaacaaacg gcaggataat caggctaccc accagcttaa acagcaggct
    13081 acccagcgcg acacggcggg ctgcggcatt ggcggcactg ttgttgagca tcgccagcag
    13141 gccggaaccg aggtttgcac caatcaccag acaaagcgcc actgggaaag agataatgcc
    13201 tgcggcggtc agagtcgcgg tcagcagtac cgcggcaagg ctggagtagc tgataatggc
    13261 gaacatcgca ccaatcagcg catccagcag aatatcgccg gtcagcgagg caaagatcac
    13321 ctgaacgccg tttgcctggg tgatcggcgt tacggcctgc acaatcaact ccagcgccag
    13381 caaaatcaac ccaagaccaa taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg
    13441 tccgaggaaa aaaatcacgc cgataaaaat aagtaatggt gacagccagg ataagtcgaa
    13501 ggtgagaata cgcgccatta gcgccgtccc gacatctgca cccagcacaa tcaccagagc
    13561 cggtgcgagg gctaccagat cctgagcgac gaacgaggtc accagcatag tggtggcatt
    13621 actgctctgc accagtgcgg taacgcctat ccccgcgcaa aaggcgagcg gctttttttc
    13681 gacgctccgg ctaaggacag tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg
    13741 aacaatatga gtcccccaga ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa
    13801 cacgagttca ggttctcctt atcgttatat gttcctgaag caattgaatg accaaaggac
    13861 cggaaacacg ctgactggca tctttactgt gccttttgac cttcgttttt ccggctaaga
    13921 ttattccagc ctgtttccag tataagtgtt tcatattaat aaagagacag ggcactcatt
    13981 gagcgccctg tttgttgtaa ggaaaagtga cagatttgtg aatcagtccg cgtcataccc
    14041 cagattcggt gccagccagc gctcaacatc tgagacgctc atacctttac ggcgggcata
    14101 gtcttcaacc tgatcgcgct ggatttgcgc caccgcgtaa tacttgctgt ccgggtggct
    14161 gaagtaccaa cccgaaaccg atgcaccagg ccacatggcg aaagattctg tgagtttcat
    14221 gccagtgtgt ttttccactt ccagcagctc ccagatggtg gctttttccg tatgttccgg
    14281 gcaggccgga taacccggtg ccggacggat gccctggtag ttttcgcgga tcagctcttc
    14341 gttgctgagg ttctcgttcg gcgcatagcc ccagtagact ttacgcacac gctcatggag
    14401 atactccgca aaggcttcgg ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta
    14461 atcatcgtgc tgcgcttcaa aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac
    14521 ggcaaatgcg ccgatgtaat ctgctttacc agaaagcttc ggcgcaacga agtcagcgag
    14581 acagtagtta gcgaagccgg ttttttcggt ctgttgacgc agatggtggc tgacgttgat
    14641 cacatgggta cgcgtttcgt cacggtagat ttcaatgtca tcgcccacac ggtttgccgg
    14701 gaacaggccc accacgccac gcggattcag cgttttttcg gcgcttaatt tatccagcat
    14761 gtcgttggcg tctttaaaca gccgctgcgc ctcaacgccc accacttcat cttccagaat
    14821 gcgcggatac ttcccggaca gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt
    14881 acgcagcgtt tcgatgctgg cttcgacttc ctgcacaccg agacggtgcg ccaccggcgg
    14941 cgtgtaagcc tgccagtcaa aagcgaagtc gttatcacgc gccgcttcca gcgtgaccgg
    15001 tggtgtgcgt ggtttctttc gtccgtgctg aatacgcacg gtttcgtact ccttgcgggt
    15061 acgagcaacg aaatcatcgc gctgagtgtc ggaaagcaac gccgccacca caccaacggt
    15121 gcgtgaagcg ttctgcacat acaccgtcgg gccgctgtag ttctgctcaa ttttcaccgc
    15181 cgtgtgcgct tttgaggtgg tcgcgccgcc aatcaatagc ggaatggtga agccctgacg
    15241 ctccatctct ttcgccacgt taaccatctc gtccagcgac ggcgtgatca gccccgaaag
    15301 gccaatcaaa tcagcattca cttctttagc ggtacggaga attttttccg caggcaccat
    15361 cacgccgaga tcgacaattt cgtagttgtt acattgcagc accacgccaa cgatattttt
    15421 accgatgtcg tggacatcgc ccttcactgt ggcgatcacc atcttgccgt tggttttacc
    15481 ctgctctttg ctggcttcaa tgaacggctc gagatacgcc accgcctgtt tcatgacgcg
    15541 cgccgatttc accacctgcg gcaggaacat tttgccctcg ccaaacaggt cgccgaccac
    15601 gttcatgccg tccatcaacg ggccttcaat cacttcaatc ggacgcgtag cctgctggcg
    15661 ggcttcttcg gtatcctgct cgataaactc ggtaatgcct ttgaccagcg agtattccag
    15721 acgtttattc acttcccacg agcgccactc tgcctgctgg gtgttagcgg tgtcgtcggt
    15781 tttgctgccg cgatatttct cggcaagctc gagtaaacgc tcggtgccat cgtcgcgacg
    15841 gttgaggatc acatcctcca ccgcgtcgcg tagctcggta ggcaggtcgt cgtaaatcgc
    15901 cagttgcccg gcgttgacga tccccatatc catgccattg cgaatagcgt agtagaggaa
    15961 caccgcgtga atggcttcgc gcaccggatc gttgccacgg aatgagaaag aaacgttaga
    16021 aacgccgccg gaaatcagtg cgtgcggcag ttcacgtttg atgtcttcac acgcgccgat
    16081 aaagtcctgc gcgtaattgt tgtgctcttc aatgccagtt gcgaccgcga agatgttcgg
    16141 gtcgaagatg atatcttcag ccgggaagcc aacctcttcg gtgaggattt tgtacgcccg
    16201 acggcaaatc tcgattttcc gtgcgcgggt atcggcctgg ccctgttcgt caaaagccat
    16261 taccacgact gccgcaccgt agcgacgcaa cagtttcgcg tgatggataa aggcgtcgac
    16321 gccctctttc atcgagatag agttaacaat gcctttgccc tggatacact tcagaccttt
    16381 ttcaataacg tcccattttg aggagtcgat cattatcggc acgcgagcga tatccggttc
    16441 accggcaatc agattgagaa aacgcaccat cgccgcttcg gcgtcgagca tcccttcatc
    16501 catgttgata tcgataatct gcgcaccgtt ttccacctgc tgacgcgcca catccagcgc
    16561 ctcgctgtat ttctcttctt tgatcaggcg cttgaactta gcggaaccgg tgacgttggt
    16621 gcgttcaccc acgttcacaa acaggctatc ttcgccaatg ttcagcggct ccaggccgga
    16681 caaacggcag gctacgggga tttccggcag tttgcgcggc gctaatcctt ctactgcacg
    16741 actcatcgcg gcaatatgtt gtggcgtggt gccacagcag ccgccgacga tattgagaaa
    16801 gcccgcttgc gcccattcac gtatctgttt tgccatcgtg tcggcgtcga gatcgtactc
    16861 accaaaggcg ttgggtagcc cggcgttcgg gtgcgctgtg acgtagcatt ccgcaatccg
    16921 tgataattcc tgcacgtact ggcgcagttc gtctggcccc agcgcacagt taaggccgaa
    16981 agtcagcgct tcggcgtggc gcagagagtt ataaaacgct tcggtggtct gcccggagag
    17041 cgtgcgcccg gaggcgtcgg tgatggtgcc ggagatcata atcggcagct caacgcccag
    17101 cgcttcaaac tccgttttca ccgcaaagac cgccgctttg gcgttaaggg tgtcgaaaac
    17161 ggtttcaatc agaatcaggt cggcaccacc ttccaccagc gctttggtgg actctcgata
    17221 agccgccacc agcccgtcaa aagtgatatt acgaaatgcc ggatcgttga catccggaga
    17281 aatagacgcc gtgcggttgg tcgggccgag aacaccggca acgtagcgcg gtttctccgg
    17341 cgtgcgcgcg gtccactcat cagcacaggc gcgcgccagt ttcgccgccg caaagttgat
    17401 ttccgccgac aggaattcca tctggtaatc cgccatcgca atggttgtgg agttgaaggt
    17461 gttggtttcg atgatatccg cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac
    17521 ttccggtttg ctgagcacca gcaggtcgtt gttgcctttg agatcgcatg gccagtcggc
    17581 aaagcgttca ccacgaaaat cggcttcgtt cagtcgatag ctctggatca tggtgcccat
    17641 accgccgtcc agcaccagaa tacgttcatt taactgcgca cgcagttgtt ccactttgct
    17701 gctcacactt gctcccgaca cgctcaaccc agatttaata aaaattcaac aaaccatact
    17761 ggcataaacg catctgtggt aaaagcgacc accacgcaac atgagatttg ttcaacatta
    17821 actcatcgga tcagttcagc aactattgca ttagctaaca ataaaaatga aaatgatttc
    17881 cacgatacag aaaaaggaga ctgtcatggt cgcacccatt cccgcaaaac gcggcagaaa
    17941 acccgccgtt gccaccgcac cagcgactgg acaggttcag tctttaacgc gtggcctgaa
    18001 attactggag tggattgccg aatccaatgg cagtgtggca ctcacggaac tggcacagca
    18061 agccgggtta cccaattcca cgacccaccg cctgctaacc acgatgcagc aacagggctt
    18121 tgtccgtcag gtcggcgaac tgggtcactg ggcaatcggc gcacacgcct ttatggtcgg
    18181 tagcagcttt ctccagagcc gtaatttgtt agcgattgtt caccctatcc tgcgcaatct
    18241 aatggaagag tctggcgaaa cggtcaatat ggcggtgctt gatcaaagca atcacgaagc
    18301 gattattatc gaccaggtac agtgtactca cctgatgcga atgtccgcgc ctatcggcgg
    18361 taaattgccg atgcacgctt ccggtgcggg taaagccttt ctggcccaac tgagcgaaga
    18421 acaggtgacg aagctgctgc accgcaaagg gttacatgcc tatacccacg caacgctggt
    18481 gtctcctgtg catttaaaag aagatctcgc ccaaacgcgc aaacggggtt attcatttga
    18541 cgatgaggaa catgcgctgg ggctgcgttg ccttgcagcg tgtattttcg atgagcaccg
    18601 cgaaccgttt gccgcaattt caatttccgg accgatttca cgtattaccg atgaccgcgt
    18661 gaccgagttt ggcgcgatgg tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg
    18721 aatacgctga ctttttcgcc tctgatttat acgaatcgaa cgctaaaccg ctgcttgcgc
    18781 cgatacgcat aaacatcttc cacatgccct tcacgtatgc ggttttgtag tgcgcgccag
    18841 taatcagcgc ggaacaagtc ggcgtgcatc tcttcaaata gcggaccaat acgcgggtcg
    18901 gcacataacc agtgacggaa ctcttccggg aaaacatcgc ccggcgagac gctgtaccac
    18961 ggttcgctgg caagttcgtc ctccgggtag cgcggcggcg ggatgtcgcg gaagttcact
    19021 tccgtcatgt agcaaatttc gtcgtaatca taaaacacca cgcgcccgtg acgggtgaca
    19081 ccgaagtttt taaacagcat gtcgccaggg aaaatattag cggcggcaag ctggcgaata
    19141 gcgttaccgt attcttcaat ggcatcgcgc aactgctgac cttccacttg ttccagccag
    19201 atattgagcg gcaccatccg ccgctcaata taaagatggc gaatcacaat ttgttcgccg
    19261 agatcggtga ttttttccgc tgcttcctgg agcagtaatg ccattaatgc cggggaaata
    19321 tgccgcttct ccagcacaaa gttttcaaac tcctgggtgt ccgccattcg gcccacgcga
    19381 tcgtgctctt tcaccagttg atagcaggca cgaacgtgag cggcagacat ctctttctgc
    19441 ggcgcgaact tgtctttgat gactttgaat acccgatcaa agcccggcag cgtaaacacc
    19501 agcatcacca tgccacgaat acccggcgct tcaatgaact gctcattaca gccctgtaga
    19561 taaacgagat attcgcggta gctttcggtt ttggcgtgct tctggcagcc gatagccata
    19621 tacaattcag cggtggtttt acctggcaga atttcccgta gccactcgac cagcgctgcg
    19681 ggcagcggtg cataaaccat aaaataagaa cgcgcaaagc caaaaacaat gctcgcttcg
    19741 gcggtggtcg tcaggcaggt atcaataaat aactcgccgt cgtccgtctg gtggatcggc
    19801 agcaaaaatg gcaatgtgcc ggaaggcgtg atcagattgc ctaccagcca ggcggcttta
    19861 ttgcggtaaa acagttcgtt cgccacctgt aaatgacttt ccgcgaggtt gtctgtcccc
    19921 agcgtttccg tcagatggcg aacgatgtaa tgaatgtcac ggcttttatt ctgccaacgc
    19981 agacgcagcg gcagatcgct gataacgcgc atcagcagag attcccagcc gtgatcgggg
    20041 tgaaagtctt tcgccagcgg gcggggaatg gtacgaaaac ggcgctctgg ctgggagcta
    20101 aagataaaaa gccgctcggg agtaagcgag cggtggtcaa ataacctaca gtacacggag
    20161 ttgaaaaagc tctccgcaat ctcgaagcgc gggtaatccg gcaacagccg cgtgtaatgc
    20221 tctttgacgc gcagtaaaaa tgccgcgtcc gtgctttggc cgttggtaat gcagcgcagt
    20281 tgctccacga ccagacccac gtgatgatcg taaagatgaa tacggttttt catcgcctgc
    20341 tggacagcat gccagtcggc ttgctcgaaa cgctgctgcg ccccggaagt cacttcgagg
    20401 aatcgaccat actgagcgtc gaaaccttgc aaaatggttt gtgcaatcag taattccagg
    20461 ccacgcggca tttgttgccc tcgtcgggag cagagaattg ccggataaga cgcgtaagcg
    20521 tcacatcagg cgctgattgc cggatgcggc gtagacgcct tatccggcct acgttcggca
    20581 acggctgtag gcatgataag acgcgcaagc gtcgcatcag gcgctgattg ccggatgcgg
    20641 cgtgaacgcc ttatccggcc tacagtcagc aacgtttatc ggttgcttag aactgcgatt
    20701 cttcggtgga gccagtcagt gcggtaaccg acgacgtgcc gccctgaata atggtcgtca
    20761 ctttatcgaa gtaacctgta cccacttcct gttggtgaga tacgaaggta tagccatctt
    20821 tcgcggcggc aaattccggc tgctgcactt tctcaacata gtgcttcata ccctcgccct
    20881 gggcataggc gtttgccagg tcaaacatgt tgaaccacat gctgtggata cctgccaggg
    20941 tgatgaactg gaacttgtag cccatatccg acagctgctg ctggaagctg gcaatagttt
    21001 tgtcgtcgag gtttttctgc cagttgaacg acggcgagca gttataagcc agcagtttgc
    21061 ccggatattt cgcgtggatg gcttgtgcaa agcgacgcgc cagttccagg tccggcgtgg
    21121 aggtttcaca ccagaccagg tcagcatatg gcgcatacgc caggccacgg ctgatcgctt
    21181 gctcaatacc cgcatgagta cggaagaagc cttcactggt acgctcgcca gtaataaatt
    21241 cgctgtcata tgggtcgcaa tcggaggtaa tcagatccgc cgcatcggca tcggtacgag
    21301 caaccagcag ggttggaacg cccgtcacat cagctgccag acgcgccgcg accagtttct
    21361 gaatagcttc ctgagttggc accaaaactt tgccgcccat gtgaccacat ttcttcactg
    21421 atgccagctg atcttcgaag tgaactgccg ctgcaccggc ttcaatcatc gctttcatca
    21481 gttcaaaggc attcagtaca ccgccaaaac cggcctccgc atcggcgacg atcggcagga
    21541 agtaatcgac atagcgcgga tcgcccggct caatgcccgc ggaccattgg atctgatccg
    21601 cacggcggaa agtattgttg atccgctcca ccaccgctgg caccgagttt gccggataga
    21661 gcgactgatc cggatacatg ctggccgcca ggttagcgtc cgccgctacc tgccatcccg
    21721 acagatagac tgcttcaata cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg
    21781 caccgaggct gttgatgtag ccttttttcg actcgccgtg cagcagacgc cacattttcg
    21841 ccgcgcccag ttgcgccagg gtgcattccg gattgactga accgcgtaat ttcaccacat
    21901 cttccgcgct gtacgggcga gtaatgcctt cccaacgcgg ttgagtccac tctttctgta
    21961 attcttcaat ttgttgtgta cgggttttca tgtgcagatg ctccatagtt atgtggtggt
    22021 ttacgctaac aggcggtagc ctggcagggt caggaaatca attaactcat cggaagtggt
    22081 gatctgttcc atcaagcgtg cggcatcgtc aaaacgcccc tgggagaaac gttcttcgcc
    22141 cagttcgctg gcaatgactt tcatctcttc gcccagcatc tggcggaaca aggctttagt
    22201 caccggtttg ccattgctca acgttttttg atgatggatc cactgccaga ttgaggtacg
    22261 ggaaatttca gccgtcgccg catcttccat caggccataa atcggcacac agccattgcc
    22321 agagatccac gcttcgatgt actgcacagc cacgcgaatg ttggcgcgca taccttcttc
    22381 ggtgcgttca ccatcacaag gtgccagcag ctgatcggca gtaatcggcg cgtcttgttc
    22441 gcgcatcact tcaagctgat ttttacggga gccgagaatg tcgttgaata ccgccattgc
    22501 cgtatccgca aggcctgggt gggcaatcca tgtgccatcg tgaccgttat tagcttccag
    22561 cgatttatcc gcttttactt tgttgagcac ctggttattg cgctcttcat ctttgctcgg
    22621 aataaatgcc gccataccgc ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcaa
    22681 caggcgtgag taagcattca ggaagggttt atccatcgtc actgcctgtc tatctggcag
    22741 gacgcgatcg ggatagtttt tcaatgtttt gatataactg aagatgtaat cccaacgacc
    22801 gcagttcaga ccaacaatat ggtcacgcag cgcgtgaagg atttcatcca tctggaacac
    22861 ggcgggcagc gtttcaatca gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg
    22921 atcttctgca tagctgaaga cttcgctcca ccaggccgct tcctgccagg actgggtttt
    22981 cggcagatag aaatagggac cgctgccctt tgccaacagt gcctgatagt tgtggaagaa
    23041 atagagcgca aaatcaaaca ggctaccggg gattgcctca ccacgccagg tgacatgttt
    23101 ttccggcaag tgcagaccgc gtacccgaca aatcaaaacc gctggattgg gcttgagctg
    23161 ataaattttg cctgcttcat tggtatagct gatggtgccg ttaaccgcat cgcgcaggtt
    23221 aatttgccca tcgatcactt tgttccagtc tggtgccagt gaatcttcga aatcggccat
    23281 aaagactttc acattggcgt tgagcgcgtt gatcaccatc ttgcgctcaa ccgggccggt
    23341 aatctctacc cgacgatctt ctaagtccgc aggaatcccg cgaattttcc agtcagcatc
    23401 acgaatggaa gctgtttccg aaataaaatc aggcaacgtt ccgttatcga tatcctgctg
    23461 ctgctgaatg cgcgctgcca gaagtttatt gcgttgtggc gtaaaatgcg tcaccagctc
    23521 agtcagaaat tctaccgctt cggcagtaag aatatgcttc tcctgctcgc catacggcct
    23581 tgtgaaagcc aattcatcgg ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat
    23641 ggatccgaaa acttccccac tgtgaacgaa ggatcgttgt gcgcttttcg ttcagcacaa
    23701 ctaagactac tcatttaaaa tgcaaaatca aaaacaattt ccattttaat taaaaatact
    23761 tgataactta ttgataacaa tatgaataaa atttattcac ctactgaggt gcgtttcgga
    23821 aataaaaaag gcacccgaag gtgcctgagg taaggtgctg aatcgcttaa cgatcgacta
    23881 tcacagaaga ttaatccagc gttgggttca tgtgccgtag atcgtatggc gtgatctggt
    23941 agacgtaata gttgagccag ttggtaaaca gcaaattacc gtgactacgc cagctcgctc
    24001 gcggtgtatt ttgcggatca ttatgcggga aatagttata cggtacatcc gggtctagtc
    24061 cggcttccac atcgcggaaa aattcctgcg ccagcgtttg cgcatcatat tcgggatggc
    24121 ccgtcacaaa ggcaattcgc ttatctttgc tggcaaacag ataggcatcc ccttcttctg
    24181 tctccgccag aatttccaga tcggtataat cacgaatcaa cgctgccgga aagtcagcat
    24241 agcgcgaatg tggtgccagg aatgaatcat caaagccacg cgtcagaagc gcatgaggat
    24301 ggagaatatg atgctcgtaa acgccagaga gtttgtcggt gcgggtttgc ttaggaatgc
    24361 cgtagaggat attgagcgcg gcctggaccg cccaacagac aaacagcgtc gaggtgacgt
    24421 gatctttcga ccactccagc acctgtttga tctgcggcca gtaagcaaca tcattaaact
    24481 ccaccaggcc cagcggcgcg ccggtaacta tcagaccatc aaagttttgt tcctgaatat
    24541 cttcaaagtt acagtagaag ttattcagat gctctgcggg cgtgttgcgc gattcacggg
    24601 aatcgatgcg caacagctga atatcgacct gcaaaggtga gtttgaaagc aggcgcagaa
    24661 actgattttc agtttcgatc ttcttcggca tcaggttaag gataagcacc tttagcggac
    24721 gaatttcctg accagacgca cgagaagttg tcatcacaaa gacgttttct tcacgcaaga
    24781 aattgacggc gggtagctcg tccggcacac gaatcggcat aacctgatta cctcactaca
    24841 tacgcttata cgtttagaca tccagatagc tgaagataac cagaaaattt gccaatgtcg
    24901 agcctgcata ttgaaggtga gaaagtttca aggaaaaggc tacgttagaa tataagaatg
    24961 acgaaaagga gagaaaatgg ttattagtat tcgccgctca cggcatgagg aaggggagga
    25021 gctcgttgcg atttggtgtc gttctgtcga tgccactcac gattttttat cagcagcgta
    25081 tcgggccgag ctggaggagc tagttcgttc cttcctgccg gaagcgccgt tgtgggtcgc
    25141 ggttaatgag cgggatcggc cggttgggtt tatgttgtta agtgggcagc atatggatgc
    25201 gctgtttatc gatcctgatg tgcgcggctg cggcgtgggt cggatgctgg tggaacatgc
    25261 gctctcgatg gcaccggaac tgacaaccaa tgttaatgag caaaataagc aggctgttgg
    25321 gttctataag aagatgggtt ttaaggttac gggacgctct gaggtggacg atttggggaa
    25381 accgtatcca ttgctgaatc tggcatatgt aggggaataa gctgccccct atcgtgctat
    25441 cgtgcttgcg taagctggag cagacgcgcc agcgtcgcat ccggcatttt ttctgcgcca
    25501 atgcaaaaag gccatccttt cggatggcct tt
//
LOCUS       DAZGHF010000070        25403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGHF010000070 DAZGHF010000000
VERSION     DAZGHF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.070"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(225..1037)
                     /locus_tag="ACHK3A_002889"
     CDS             complement(225..1037)
                     /locus_tag="ACHK3A_002889"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO8740945.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRN"
     gene            1299..2063
                     /gene="udp"
                     /locus_tag="ACHK3A_002890"
     CDS             1299..2063
                     /gene="udp"
                     /locus_tag="ACHK3A_002890"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO8740946.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(2097..3587)
                     /locus_tag="ACHK3A_002891"
     CDS             complement(2097..3587)
                     /locus_tag="ACHK3A_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFO8740947.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(3600..4106)
                     /locus_tag="ACHK3A_002892"
     CDS             complement(3600..4106)
                     /locus_tag="ACHK3A_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFO8740948.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(4123..5097)
                     /locus_tag="ACHK3A_002893"
     CDS             complement(4123..5097)
                     /locus_tag="ACHK3A_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFO8740949.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMAYSKANPGKVRIGN
                     SGTGAIWHLAVAALEDKTGTKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(5122..5751)
                     /locus_tag="ACHK3A_002894"
     CDS             complement(5122..5751)
                     /locus_tag="ACHK3A_002894"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO8740950.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYRPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(5741..6745)
                     /locus_tag="ACHK3A_002895"
     CDS             complement(5741..6745)
                     /locus_tag="ACHK3A_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO8740951.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEHGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(6769..7536)
                     /locus_tag="ACHK3A_002896"
     CDS             complement(6769..7536)
                     /locus_tag="ACHK3A_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO8740952.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            7753..9180
                     /gene="rmuC"
                     /locus_tag="ACHK3A_002897"
     CDS             7753..9180
                     /gene="rmuC"
                     /locus_tag="ACHK3A_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO8740953.1"
                     /translation="MDFSIIVYAIIALVSVVIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            9275..10030
                     /gene="ubiE"
                     /locus_tag="ACHK3A_002898"
     CDS             9275..10030
                     /gene="ubiE"
                     /locus_tag="ACHK3A_002898"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO8740954.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            10044..10649
                     /gene="ubiJ"
                     /locus_tag="ACHK3A_002899"
     CDS             10044..10649
                     /gene="ubiJ"
                     /locus_tag="ACHK3A_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO8740955.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            10646..12286
                     /gene="ubiB"
                     /locus_tag="ACHK3A_002900"
     CDS             10646..12286
                     /gene="ubiB"
                     /locus_tag="ACHK3A_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO8740956.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            12365..12634
                     /gene="tatA"
                     /locus_tag="ACHK3A_002901"
     CDS             12365..12634
                     /gene="tatA"
                     /locus_tag="ACHK3A_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO8740957.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            12638..13153
                     /gene="tatB"
                     /locus_tag="ACHK3A_002902"
     CDS             12638..13153
                     /gene="tatB"
                     /locus_tag="ACHK3A_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO8740958.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            13156..13932
                     /gene="tatC"
                     /locus_tag="ACHK3A_002903"
     CDS             13156..13932
                     /gene="tatC"
                     /locus_tag="ACHK3A_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO8740959.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            13974..14756
                     /gene="tatD"
                     /locus_tag="ACHK3A_002904"
     CDS             13974..14756
                     /gene="tatD"
                     /locus_tag="ACHK3A_002904"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO8740960.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKVPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(14753..15241)
                     /gene="rfaH"
                     /locus_tag="ACHK3A_002905"
     CDS             complement(14753..15241)
                     /gene="rfaH"
                     /locus_tag="ACHK3A_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO8740961.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            15408..16901
                     /gene="ubiD"
                     /locus_tag="ACHK3A_002906"
     CDS             15408..16901
                     /gene="ubiD"
                     /locus_tag="ACHK3A_002906"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO8740962.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            16947..17648
                     /gene="fre"
                     /locus_tag="ACHK3A_002907"
     CDS             16947..17648
                     /gene="fre"
                     /locus_tag="ACHK3A_002907"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO8740963.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(17840..19003)
                     /gene="fadA"
                     /locus_tag="ACHK3A_002908"
     CDS             complement(17840..19003)
                     /gene="fadA"
                     /locus_tag="ACHK3A_002908"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO8740964.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMIGDAQTCLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLTQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISSTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(19013..21202)
                     /gene="fadB"
                     /locus_tag="ACHK3A_002909"
     CDS             complement(19013..21202)
                     /gene="fadB"
                     /locus_tag="ACHK3A_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO8740965.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYSGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMEQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKMA"
     gene            21392..22723
                     /gene="pepQ"
                     /locus_tag="ACHK3A_002910"
     CDS             21392..22723
                     /gene="pepQ"
                     /locus_tag="ACHK3A_002910"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO8740966.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENITRDLKLA"
     gene            22723..23337
                     /locus_tag="ACHK3A_002911"
     CDS             22723..23337
                     /locus_tag="ACHK3A_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO8740967.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            23376..24827
                     /gene="trkH"
                     /locus_tag="ACHK3A_002912"
     CDS             23376..24827
                     /gene="trkH"
                     /locus_tag="ACHK3A_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO8740968.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            24839..25384
                     /gene="hemG"
                     /locus_tag="ACHK3A_002913"
     CDS             24839..25384
                     /gene="hemG"
                     /locus_tag="ACHK3A_002913"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO8740969.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         5993 a         6500 c         6854 g         6056 t
ORIGIN      
        1 gttgataata attaatgttt gtttgagaat gggagttgat ggtaaatctc taaggtgtct
       61 gccagtgaag agactcattt atgactatga cttgccgact ttcttaaggt caaatagcgg
      121 agtcgtgctc tccaatcaat tcatactgat tattttttgg gggacagaga tggcagcaaa
      181 atgcactcgt tcactcacca tctcaaatat agactaagag gaaactaatt ccgcttccca
      241 ccatactgct taaaccactc cagcatacgc tgccagccat ctttcgcaga ttcggcatga
      301 tagctcgggc gataatcggc gttgaatgca tgcccggcat ccgggtagac gataatctct
      361 gctttcgcat tagcagcccg cagcgcctgg cgcatggttt caacgctctc ctgcggaatg
      421 ctgttatcct gaccaccata taagccgaga accggcgcgt taagatcggt tgcgatatca
      481 acaggttgtt tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact
      541 gcggctttta gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag
      601 aatccggtga tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca
      661 tgatcgagat cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac
      721 gtggggatat cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcaata
      781 gccagatacc cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca
      841 aaaatttcct gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca
      901 tggtaagcag gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg
      961 tcgtccggag tctgaacgat ggtcgaagcg agaggcgatg ccgcaggtgc aaatccagat
     1021 tgttgtgttg ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact
     1081 atagtcagca tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca
     1141 gaatgaagag tgatttatgt gatttgcatc acttttgatg ggtaaattta tgcaacgcat
     1201 ttgcgtcatg gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt
     1261 gcttcttctg actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc
     1321 atctcggcct cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc
     1381 cggatcgtgt ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc
     1441 gcgaattcac cacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg
     1501 gtatcggcgg cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca
     1561 ccttcctgcg tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc
     1621 tggttaccac ggcgtctgtt cgtctggacg gcgcgagcct gcacttcgca ccgctggaat
     1681 tcccggctgt cgctgatttc gaatgtacga ctgcgctggt tgaagcggcg aagtcaattg
     1741 gtgccaccac tcacgttggt gtgacggctt cttctgatac cttctacccg ggtcaggaac
     1801 gttacgatac ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc
     1861 aggcgatggg cgtaatgaac tatgaaatgg aatctgcaac cctgctgacc atgtgtgcaa
     1921 gtcagggcct gcgtgccggt atggtagcgg gggttatcgt taaccgcacc cagcaagaga
     1981 tcccgaatgc tgagacgatg aaacaaaccg agagccatgc ggtgaaaatc gtggtggaag
     2041 cggcgcgtcg tctgctgaaa taaaaaacgc tccccaactt cggggagcga tatcagttac
     2101 tgacgccgta aacgcattat tttgcggatt attggcgtca gtagcgtaaa ggctgccaga
     2161 gttagcaatg ccagagtgat cggtcgggta tacagaaagt ccagtgaacc ccctgaaagc
     2221 gacagcgcac ggcgaaaatt actttctgcc atcgggccca taatcaatgc cagcactacg
     2281 ggtgatgccg gatatcctcc tttctgcatg aaatagccca ccacgcctgc aatcagcata
     2341 atgccaacat caaacaggct attgttcaac gcataagtcc cgaccacaca aagtgcgagg
     2401 atcatcggtg tgagaatatt gccggggatc ttcaccacgt taccgatgac tttcaaagag
     2461 agcagtccaa gaactagcag catgaagtaa caaaagatca tgccgacaaa cagtgtataa
     2521 accatatcgc cgtggtcggt aaacatcagc gggcctggtt gcattccctg taacgttaac
     2581 gcgcccagca tgatggcggt taccgcatcg ccagggatcc ccagcgtcag cataggtaac
     2641 aatgcgccac cggtgcagcc attcgcgcct gcctcacagg aggccacagc tttaatttca
     2701 cccttaccaa agttttctgg tgtcttactg aaacgacgag tttcgttata ggcaacaaaa
     2761 gctgcaatat ccgcccccgc accggggatc atgccgataa aaacccctaa tcctgatgag
     2821 cgcaaaatgg ttaatgcaat gcggcgtagt gtttgccagg gcagtaacag actgccgata
     2881 gccaccttcg ccccctgacg aatttgctgg ttttgctcca tagagcggaa tgcttctgaa
     2941 gcagcaaata aaccaatcat caccgggata aatggcacat tcatcagctc cacaaatcca
     3001 cctgtaaacc gtggaaaacc gcccattgga tcaaggccta tcgttgcgat cagcaatccg
     3061 ccaaccccgg caatcagccc tttaatcagc gactcaccgg aaatactggc aataatactc
     3121 agtccgaagg ttgccagggc gaaggactcc gaggcactaa atttcagcgc ccaactggca
     3181 agtaccggag agagaaacat cagcacgata atgctgagcg ttccgccact aaaggaagag
     3241 aacgtggcgg cactcaatgc cagccctgct tttccctgtt tcgtcagctc ataaccatca
     3301 atagcggtgg ctgcggcggc aggcgttccg ggaattttga gcaaaattgc ggtgatagaa
     3361 ccaccgtaga cgccaccaaa aaagacgccg caaatcatca acaagccaga aacaggctcc
     3421 atgccgtagg tgaaaggcag caaaatcgcc acacccatgg tcgctgtcag accaggcaaa
     3481 gcgccaatca caatacccag catcacccca aaagtggcaa acagcagcgc ctgaggatta
     3541 ttgagtaatg tcgaaaaacc ctgagttaaa agttcacttt caaacattgc tggcccctgt
     3601 tattcgaaaa atgtgccgac aggcaatggc acctgtaacg cttcgacaaa cagcaaccag
     3661 aggattgccg ttaacagcag cgcaataccc ggtgtgagta cccatcgcct gtagcccatc
     3721 aacgtcataa tgacgatgag gaaaacagga gtagtaatga gataaccgca gtagctcatc
     3781 gccacaatat aaaacgcggt tgccgcaatt cctgttgctg ttttcctgaa tgccggtttt
     3841 tcctgtgcgt tggcgcaaga ggctggtttt gcctgcaaaa gatcgcgtat caccagcaag
     3901 acagcgagca caatcagggc acaggcatag aaggtaggga aggcacttgc accgggatcc
     3961 gtttcaatgg cagttgctgg aaaatcagcg gcattaatca tcaccgcgcc agcaaccaca
     4021 caaagtaaca gcgccaccac catcgttgat gaaaggcgct tgcggtgcgg tacggcgagt
     4081 tgagcaacag actgttttgt gttcatcaac cgtacctcag gtttattttt tcaggcccag
     4141 gcgagtcagc aactcgtcaa agtacttttc ctgttcgctg atttgggtct ggaagctggc
     4201 agcgtcaagc catgcatagt tgagattcaa cttttgcagt gcatcctgga atgcaggctc
     4261 ttcagccgtc tcttttgctg cctttgccag aacatccacc acatcctgcg gcgttttttg
     4321 cgacacaatc aggccgcgcc aggtgccgat ggagagatca acgcctttct cttttaacgt
     4381 cgggacgtca ggcatggttt tcattcgctc atccgccatc actaccagtg tcttcagctt
     4441 gccgccattc acatggttga taacttctcc tgggcttacg gaaaccgctt caatatgccc
     4501 gcctaacagg cctgtaatgg caggggctgc gccgtcatac gggacatgag aaaactttgt
     4561 gcccgttttg tcttccagtg cagctaccgc cagatgccag atagctccgg tgcctgagtt
     4621 accaatgcgt acttttccgg gattcgcttt ggagtaagcc ataaattctt catagctatt
     4681 ccacggcgca tcggcacgga ctgtgatagc agccggatcc gcattcagac gggcaatggg
     4741 tttaaagtcg ctggttttaa aacgcaccat tccgaggctg ggaagggtgg tcagttcaac
     4801 cgtgcctaaa ccaattttgt aaccgttagg gcgggcagcg gcgatttcac tcaggccgat
     4861 agcaccgcct ccgccaggtt tattgataac cccgatgctg acgggtaaat ggtttttggc
     4921 ggcatcagca aaggcacggg caaccagatc cgtaccgcct ccggcagcgt agggaacgac
     4981 taactcgatc tgtttggttg ggtaggaggc agcctgtaca ggcgcaatgc tgagggcagc
     5041 gcctgcaaga agtaccattg cagccatttt tttcggtaag gtaagatgtt ttatcattgc
     5101 ttttatatcc ttatttatca gttagttgct atttaaattg ttccatgcgt tcataaacag
     5161 tactgcattt tcatagactt gctctggcgt catgccgggg cgatatagcg cagaaccgag
     5221 gccaaagccg tttgcgccgt ggctcagata acgcgccatg gtttccggtg tgataccgcc
     5281 aaccggtaac atcggaacgg attgtggaat aaccgcacgc caggctttgg taacttctgg
     5341 cgtaatttgt tcagccggga acagcttgat ggcattagca ccatgttcaa tggcagcaaa
     5401 ggcttcggtg ggcgtggcga cgccaggcgc acaatacata cccagcgcac gggcgcgacg
     5461 gatcaccgca gtatcgcaat gcggcatcac gataatctgg cccccagcct ctttaaccgc
     5521 aaccacctgc tctaccgaaa gcacagttcc ggctccgacc aggcagtcct taggcagggc
     5581 gtcgcgcagt aaactgatac tttgcaacgc ctccggcgag ttcataggaa tctcaatcag
     5641 gcggaatccg gcggcgtaca gtttttcacc gatggcgaca atctcatccg gtttcacgcc
     5701 gcgtaaaatg gcgactaacc cggttttttg caaaagtgta ttaaatggca tgggtacatt
     5761 ccctggcgtt ggtggacagt aacccggcag cgtgggcgat tcgccataat ccatgttcag
     5821 tggcattagg aatggcgtgt atctgtctgt cgggaaaaac gtggctgaat gcttgtgaat
     5881 agcggctgtt cagatttgcg ctaccgatta aggcgatcgg tgttgtggcg ggtaaatcac
     5941 gtagcaaact ggatgccaga ccacataatt catggcctat cagtaatccg gaaaggtaat
     6001 ccccctgcga ggacgacgta agattactgg tcagcaggcg cgaacgggtc gaaaacagcg
     6061 tcgataataa tccggtctga tgatgctctt gtgctgctgt cagcccgtgg gtgaaggcat
     6121 cccagtgcgg ttcatctcca ggatgcatgg tggcaccgag aatcgaatgg cgggataaaa
     6181 catcaaataa ctcgcccgtc ataaaggttc ggaaatcggt aatggtgttg ttttcaacta
     6241 ctgcccattt cgcatgtgtg ccgggtaagc ctatcagcac tggcataccc tgatggatcg
     6301 cgttttgcag ggccggttcc atcgagatag caccgaagat ttgtgtctct tcgccacgca
     6361 tcacttcggg catttcacct tctttaatca cgccaggaat gatgtgcagc attgtctgct
     6421 gctggtggat aacaggcgtc aactgcttcg ccagaccctc gagagtgacc gggcaaggta
     6481 aataagctgc tgattgccac ccttgcgcac tgccgagcat gccgcaagcc agtaccgggc
     6541 atggggtttg tgccagccag tcgccgcaga cacgtaaaaa cgtatcgtgg aaagcgtcct
     6601 cacgtgttgc tgcctgggag ggtaatttca tgatccccca ggggaattca cgtttttctt
     6661 gtggatttgg tgattcgcca aaacgccatg cccgaagcga tgacgttccc cagtccaggg
     6721 caataaatga gggtgctccc ggcattagtc gtttccttta atcatggttt atttatcttt
     6781 gcggacgtgc ttaccccagc ccagttcagc ggatatctct tctgcacagg atttgacgta
     6841 aggtgccagt tcttccagac gcgccagcga catatacgtg gtggtactgg cgacagaaat
     6901 agccgccaca atttcatccc gggcgttgta aaccggcgct gcaacgcaac ggatagtcgg
     6961 ctcgttctct tccagatcaa aagcaaaacc actggcggca taacgacgca tgttctgaat
     7021 aaatgcgccc agttttgttt catcaccaca ggtcttaaac agcgtgcgcc actcttcttc
     7081 tgttcggttg aggatcaatg ccttgccgat gccggtgatt gccagtggca tgcgatggcc
     7141 tgggcgtgag cgcatttcca gcccgcgctg actgttgatc ttctccagat acagcacttc
     7201 gtcgccttca acgatcccca gatgcacggt gtccagggtg taacgggcaa ggcgttgcaa
     7261 aacgggctgg gcaatttcgt acagtgaggt gctttccagt gctctggcac cgaattcgat
     7321 cagtttggca cccagttggt agccctgcgt tgggacatgg cgcaggtagc gttgttccac
     7381 cagaacggtg actaaacgat gggttgtgct ccgggtcgtg ccggtgaact cgccgatact
     7441 ttttaggtca tggcagccgt tataaacagc attaagaacc gccagtccac gtaataacgt
     7501 ttgtgcgcct gcggtggttg aagaggtttc tgccatacgt ttctcctgat gatattgctg
     7561 aagtgttcca cataatgacg tggggaaaag gtgatatatc ccacaatata aactccatct
     7621 catattgtgg gataaaataa tgaaaattaa cgatcgtttt gcgtagcgcc tcgcaggaaa
     7681 cttctttcaa actggacgtt tgtacagcac aattctattt tgtgcgggta agttgttggg
     7741 tcaggaggct ttgtggattt ctcaatcatt gtttatgcca ttattgcgct ggttagtgtg
     7801 gtcattggct ggctgtttgc cagttatcaa catgcgcagc aaaaagccga gcaattagct
     7861 gaacgtgaag agatggtcgc ggagttaagc gcggcaaaac aacaaattac tcaaagcgag
     7921 cactggcgcg ctgagtgcga gttactcaat aacgaagtgc gcagcctgca aagtattaac
     7981 acctctctgg aggccgatct gcgtgaagta accacgcgga tggaagccgc acagcaacat
     8041 gctgacgata aaattcgcca gatgatcaac agcgagcagc gcctcagtga gcagtttgaa
     8101 aacctcgcca accgtatttt tgagcacagc aatcgccggg ttgatgagca aaaccgtcag
     8161 agtctgaaca gcctgttgtc gccgctacgt gaacaactgg acggtttccg ccgtcaggtt
     8221 caggacagct tcggtaaaga agcacaggaa cgtcataccc tgacccacga aattcgcaat
     8281 ctccagcagc tcaacgcgca aatggctcag gaggcgatca acctgacgcg cgcgctgaaa
     8341 ggcgacaata aaacccaggg caactggggc gaggtggtat tgacgcgggt gctggaggct
     8401 tccggtctgc gtgaagggta tgaatatgaa actcaggtca gcatcgaaaa tgacgcccgc
     8461 tcgcggatgc agccggatgt catcgtgcgg ttgccgcagg gaaaagatgt ggtgatcgac
     8521 gccaaaatga cgctggtcgc ctatgaacgc tatttcaacg ccgaagatga ctacacccgc
     8581 gaaagcgcgc tacaggaaca tatcgcgtcg gtgcgtaacc atatccgttt gctgggacgc
     8641 aaagattatc aacagctgcc ggggctgcga accctggatt acgtgctgat gtttattccc
     8701 gttgaacccg cttttttact ggcgcttgac cgccagccgg agctgatcac cgaagcgttg
     8761 aaaaacaata tcatgctggt tagcccgact acgctgctgg tggcgctgcg cactatcgcc
     8821 aacctgtggc gttatgagca tcaaagccgc aacgcccagc aaatcgccga tcgtgccagc
     8881 aagctgtacg acaagatgcg tttgttcgtc gatgacatgt cagcgattgg tcaaagtctc
     8941 gacaaagcgc aggataacta ccgccaggca atgaaaaaac tctcttcagg tcgcggaaat
     9001 gtgctggcgc aggcagaagc gtttcgcggt ttaggggtag aaattaaacg cgagattaat
     9061 ccggatttgg ctgaacaggc ggtgagccag gatgaagagt atcgacttcg gtcggttccg
     9121 gagcagccga atgatgaagc ttatcaacgc gatgatgaat ataatcagca gtcgcgctag
     9181 cccattgggg gtagttaagc cgggtagaaa tctagggcat cgacgcccaa tctgttacac
     9241 ttctggaaca attttttgat gagcaggcat tgagatggtg gataagtcac aagaaacgac
     9301 gcactttggt tttcagaccg tcgcgaagga acaaaaagcg gatatggtcg cccacgtttt
     9361 ccattccgtg gcatcaaaat acgatgtcat gaatgatttg atgtcattcg gtattcatcg
     9421 tttgtggaag cgattcacga ttgattgcag cggcgtacgc cgtgggcaga ccgtgctgga
     9481 tctggctggt ggcaccggcg acctgacagc gaaattctct cgcctggtcg gagaaactgg
     9541 caaagtggtc cttgctgata tcaatgaatc catgctcaaa atgggccgcg agaagctgcg
     9601 taatatcggt gtgattggca acgttgagta tgttcaggcg aacgctgagg cgctgccgtt
     9661 cccggataac acctttgatt gcatcaccat ttcgtttggt ctgcgtaacg tcaccgacaa
     9721 agataaagca ctgcgttcaa tgtatcgcgt gctgaaaccc ggcggccgcc tgctggtgct
     9781 tgagttctcg aagccaatta tcgagccgct gagcaaagcc tatgatgcat actccttcca
     9841 tgtgctgccg cgtattggct cactggtcgc gaacgacgcc gacagctacc gttatctggc
     9901 agaatccatc cgtatgcatc ccgatcagga taccctgaaa accatgatgc aggatgccgg
     9961 attcgaaagt gtcgactact acaatctgac ggcaggggtt gtggcgctgc atcgtggtta
    10021 taagttctga caggagaccg gaaatgcctt ttaaaccttt agtgacggca ggaattgaaa
    10081 gtctgctcaa caccttcctg tatcgctcac ccgcgctgaa aacggcccgc tcgcgtctgt
    10141 tgggcaaagt gctgcgcgtg gaggtaaaag gcttttcgac gtcattgatt ctggtgttca
    10201 gcgaacgcca ggttgatgta ctgggcgaat gggcaggcga tgctgactgc accgttatcg
    10261 cctacgccag tgtgttgccg aaacttcgcg atcgccagca gcttgccgca ctgattcgca
    10321 gtggtgagct ggaagtgcag ggcgatattc aggtggtgca aaacttcgtt gcgctggcag
    10381 atctggctga gttcgaccct gcggaactgc tggcccctta taccggtgat atcgccgctg
    10441 aaggtatcag caaagccctg cgcgggggcg caaagttcct gcatcacggc attaagcgcc
    10501 agcaacgtta tgtggcggaa gccattactg aagagtggcg tatggcaccc ggtccgcttg
    10561 aagtggcctg gtttgcggaa gagacggctg ccgtcgagcg tgctgttgat gccctgacca
    10621 aacggctgga aaaactggag gctaaatgac gccaggtgaa gtacggcgcc tatatttcat
    10681 cattcgcact tttttaagct acggacttga tgaactgatc cccaaaatgc gtatcaccct
    10741 gccgctacgg ctatggcgat actcattatt ctggatgcca aatcggcata aagacaaacc
    10801 tttaggtgag cgactacgac tggccctgca agaactggga ccggtatgga tcaagttcgg
    10861 gcagatgtta tcaacccgcc gcgatctttt tccgccgcat attgccgatc agctggcgtt
    10921 attgcaggac aaagtcgctc cgtttgatgg caagctggcg aagcagcaga ttgaagctgc
    10981 aatgggcggc ttgccggtag aagcgtggtt tgacgatttt gaaatcaagc cgctggcttc
    11041 tgcttctatc gcccaggttc ataccgcgcg attgaaatcg aatggtaaag aggtggtgat
    11101 taaagtcatc cgcccggata ttttgccggt cattaaagcg gatctgaaac ttatctaccg
    11161 tctggctcgc tgggtgccgc gtttgctgcc ggatggtcgc cgtctgcgcc caaccgaagt
    11221 ggtgcgcgag tacgaaaaga ccttgattga tgaactgaat ttgctgcggg aatctgccaa
    11281 cgccattcag cttcgacgca attttgaaga cagcccgatg ctctacatcc cggaagttta
    11341 ccctgactat tgtagtgaag ggatgatggt gatggagcgt atttacggca ttccggtgtc
    11401 tgatgttgcg acgctggaga aaaacggcac caacatgaaa ttgctggcgg aacgcggcgt
    11461 gcaggtgttc ttcactcagg tctttcgcga cagctttttc catgccgata tgcaccctgg
    11521 caacatcttt gtaagctatg aacacccaga aaacccgaaa tatatcggca ttgattgcgg
    11581 gattgttggc tcgctaaaca aagaagataa acgctatctg gcggaaaact ttatcgcttt
    11641 ctttaatcgc gattatcgca aagttgcaga gctacacgtc gattcaggct gggtgccgcc
    11701 agataccaac gttgaagagt tcgaatttgc cattcgtacg gtctgtgaac ctatctttga
    11761 gaaaccgttg gccgaaattt cgtttggaca tgtgctgtta aatctattta atacggcgcg
    11821 tcgcttcaat atggaagtgc agccgcaact ggtgttactc cagaaaaccc tgctctacgt
    11881 cgaaggggta ggacgccagc tttatccgca gctcgattta tggaaaacgg cgaagccttt
    11941 ccttgagtcg tggattaaag atcaggtcgg tattcctgcg ctggtgagag catttaaaga
    12001 aaaagcgccg ttctgggtcg aaaaaatgcc agaactgcct gaactggttt acgacagttt
    12061 gcgccagggc aagtatttac agcacagtgt tgataagatt gcccgcgagc ttcagtcaaa
    12121 tcatgtacgt cagggacaat cgcgttattt tctcggaatt ggcgctacgt tagtattaag
    12181 tggcacattc ttgttggtca gccgacctga atgggggctg atgcccggct ggttaatggc
    12241 aggtggtctg atcgcctggt ttgtcggttg gcgcaaaaca cgctgatttt ttcatcgctc
    12301 aaggcgggcc gtgtaacgta taatgcggcc ttgtttaatc atcatctacc acagaggaac
    12361 atgtatgggt ggtatcagta tttggcagtt attgattatt gccgtcatcg ttgtactgct
    12421 ttttggcacc aaaaagctcg gctccatcgg ttccgatctt ggtgcgtcga tcaaaggctt
    12481 taaaaaagca atgagcgatg atgaaccaaa gcaggataaa accagtcagg atgctgattt
    12541 tactgcgaaa actatcgccg ataagcaggc ggatacgaat caggaacagg ctaaaacaga
    12601 agacgcgaag cgccacgata aagagcaggt gtaatccgtg tttgatatcg gttttagcga
    12661 actgctattg gtattcatca tcggcctcgt cgttctgggg ccgcaacgac tgcctgtggc
    12721 ggtaaaaacg gtcgcgggct ggattcgcgc gttgcgttca ctggcgacaa cggtgcagaa
    12781 cgaactgacc caggagttaa aactccagga gtttcaggac agtctgaaaa aggttgaaaa
    12841 ggcgagcctc actaacctga cgcccgaact gaaagcgtcg atggatgaac tacgccaggc
    12901 cgcggagtcg atgaagcgtt cctacgttgc aaacgatcct gaaaaggcga gcgatgaagc
    12961 gcacaccatc cacaacccgg tggtgaaaga caatgaagct gcgcatgaag gcgtaacgcc
    13021 tgctgctgca caaacgcaag ccagttcgcc ggaacagaag ccagaaacca cgccagagcc
    13081 ggtggtaaaa cctgctgcgg acgctgaacc gaaaaccgct gcaccttccc cttcgtcgag
    13141 tgataaaccg taaacatgtc tgtagaagat actcaaccgc ttatcacgca tctgattgag
    13201 ctgcgtaagc gtctgctgaa ctgcattatc gcggtgatcg tgatattcct gtgtctggtc
    13261 tatttcgcca atgacatcta tcacctggta tccgcgccat tgatcaagca gttgccgcaa
    13321 ggttcaacga tgatcgccac cgacgtggcc tcgccgttct ttacgccgat caagctgacc
    13381 tttatggtgt cgctgattct gtcagcgccg gtgattctct atcaggtgtg ggcgtttatc
    13441 gccccagcgc tgtataagca tgaacgtcgc ctggtggtgc cgctgctggt ttccagctct
    13501 ctgttgtttt atatcggcat ggcgttcgcc tactttgtgg tctttccgct ggcatttggc
    13561 ttccttgcca ataccgcgcc ggaaggggta caggtatcca ccgacatcgc cagctattta
    13621 agcttcgtta tggcgctgtt tatggcgttt ggtgtctcct ttgaagtgcc ggtggcaata
    13681 gtgctgctgt gctggatggg aattacctcg ccagaagact tacgcaaaaa acgtccgtat
    13741 gtgctggttg gcgcatttgt tgtcgggatg ctgctgacgc cgccggatgt cttctcgcaa
    13801 acgctgctgg cgatcccgat gtattgcctg tttgaaatcg gtgtcttctt ctcacgcttt
    13861 tacgttggta aagggcgaaa tcgggaagag gaaaacgacg ctgaagcaga aagcgaaaaa
    13921 actgaagaat aaattcaacc gcccgtcagg gcggttgtca tatggagtac aggatgtttg
    13981 atatcggcgt taatttgacc agttcgcaat ttgcgaaaga ccgtgatgat gttgtagcgc
    14041 gcgcttttga cgcgggagtt aatgggctac tcatcaccgg cactaacctg cgtgaaagcc
    14101 agcaggcgca aaagctggcg cgtcagtatt cgtcctgttg gtcaacggcg ggcgtacatc
    14161 ctcacgacag cagccagtgg caagctgcga ctgaagaagc gattattgag ctggccgcgc
    14221 agccagaagt ggtggcgatt ggtgaatgtg gtctcgactt taaccgcaac ttttcgacgc
    14281 cggaagaaca ggaacgcgct tttgttgccc agctacgcat tgccgcagaa ttaaacatgc
    14341 cggtatttat gcactgtcgc gatgcccacg agcggtttat gacattgctg gagccgtggc
    14401 tggataaagt gcctggtgcg gttcttcatt gctttaccgg cacacgcgaa gagatgcagg
    14461 cgtgcgtggc gcatggaatt tatatcggca ttaccggttg ggtttgcgat gaacgacgcg
    14521 ggctggagct gcgggaattg ttgccgttga ttccggcgga gaaattgctg atcgaaactg
    14581 atgcgccgta tctgctccct cgcgatctca cgccaaagcc atcatcccgg cgcaacgagc
    14641 cagcccatct gccccatatt ttgcaacgta ttgcgcactg gcgtggagaa gatgccgcat
    14701 ggctggctgc caccacggat gctaatgtca aaacactgtt tgggattgcg ttttagagtt
    14761 tgcggaactc ggtgttcttc acactgtgct taatctcttt attaataaga ttcagcaata
    14821 gcatggagcg agcctcgccg tctggctcgg tgaaaatggc ctgaaaacct tcgaatgcgc
    14881 cttcagtaat aatcacttta tcaccaggat acggcgtcga tggatcgaca atatctttcg
    14941 gtttatacac tgataactga tgaatcaccg ccgatgggac tatcgctggc gacgcgccaa
    15001 agcgcacgaa gtggctgaca ccgcgggtcg cgttgatagt cgtggtatga atcacttccg
    15061 gatcgaactc cacaaacaag tagttgggga acaatggctc actgactgca gtacgtttgc
    15121 cacgcacgat tttttccagg gtgatcatcg gtgccaggca attcacagcc tgtctttcga
    15181 ggtgttcctg ggcacgttga agttgcccgc gcttgcagta cagtaaatac caggattgca
    15241 taatgactct tatccgttta atcggggcgc aaggatagca aaagctttac gctaagttaa
    15301 ttatattccc cggtttgcgt tataccgtca gagttcacgc taatttaaca aatttacagc
    15361 atcgcaaaga tgaacgccgt ataatgggcg cagattaaga ggctacaatg gacgccatga
    15421 aatataacga tttacgcgac ttcttgacgc tgcttgaaca gcagggtgag ctaaaacgta
    15481 tcacgctccc ggtggatccg cacctggaaa tcactgaaat tgctgaccgc actttgcgtg
    15541 ccggtgggcc tgcgctgttg ttcgaaaacc ctaaaggcta ctcaatgccg gtgctgtgca
    15601 acctgttcgg tacgccaaag cgcgtggcga tgggcatggg gcaggaagat gtttcggcgc
    15661 tgcgtgaagt tggtaaatta ttggcgtttc tgaaagagcc ggagccgcca aaaggtttcc
    15721 gcgacctgtt tgataaactg ccgcagttta agcaagtatt gaacatgccg acaaagcggc
    15781 tgcgtggtgc gccttgccaa caaaaaatcg tctctggcga tgacgtcgat ctcaatcgta
    15841 ttcccattat gacctgctgg ccggaagatg ccgcgccgct gattacctgg gggctcaccg
    15901 taacgcgcgg cccgcataaa gagcggcaga atctgggcat ttatcgccag cagctaattg
    15961 gtaaaaacaa actgattatg cgctggctgt cgcatcgcgg cggcgcgttg gattatcagg
    16021 agtggtgtgc ggcgcatccg ggcgaacgtt tcccggtttc tgtggcgctg ggtgccgatc
    16081 ccgccacgat tctcggtgca gtcacccccg ttccggatac gctttcagag tatgcgtttg
    16141 ccggattgct gcgcggcacc aagaccgaag tggtgaagtg tatctccaat gaccttgaag
    16201 tgcccgccag tgcggagatt gtgctggaag ggtatatcga acaaggcgaa actgcgccgg
    16261 aagggccgta tggcgaccac accggttact ataacgaagt cgatagtttt ccggtattta
    16321 ccgtgacgca tattacccag cgtgaagatg cgatttatca ttccacctat accgggcgtc
    16381 cgccagatga acctgcggta ctgggtgtag cactgaacga agtgttcgtg ccgattctgc
    16441 aaaaacagtt cccggaaatt gtcgattttt atctgccgcc ggaaggctgt tcttatcgtc
    16501 tggcggtagt gacgatcaaa aaacagtatg ccggacacgc gaagcgcgtc atgatgggcg
    16561 tctggtcgtt cttacgccag tttatgtaca ctaaatttgt gatcgtttgc gatgatgacg
    16621 tcaacgcccg cgactggaac gatgtgattt gggcgattac cacccgtatg gacccggcgc
    16681 gggataccgt tctggtagaa aatacgccta ttgattatct ggattttgcc tcccctgtct
    16741 ccgggctggg ttcaaaaatg gggctggatg ccacgaataa atggccgggg gaaacccagc
    16801 gtgaatgggg acgtcccatc aaaaaagatc cagatgttgt cgcgcatatt gacgccattt
    16861 gggatgaact ggctattttt aacaacggta aaagcgcctg atgcgcgttt gttttgccct
    16921 atttatcgat ccgacagaga aagcgcatga caaccttaag ctgtaaagtg acctcggtag
    16981 aagctatcac ggataccgta tatcgtgtcc gcatcgtgcc agacgcggcc ttttcttttc
    17041 gtgctggtca gtatttgatg gtagtgatgg atgagcgcga caaacgtccg ttctcaatgg
    17101 cttcgacgcc agatgaaaaa gggtttatcg agctgcatat tggcgcttct gaaatcaacc
    17161 tttacgcgaa agcagtcatg gaccgcatcc tcaaagatca tcaaatcgtg gtcgatattc
    17221 cccacggaga agcgtggctg cgcgatgatg aagagcgtcc gatgattttg attgcgggcg
    17281 gcaccgggtt ctcttatgcc cgctcgattt tgctgacagc gttggcgcgt aacccaaacc
    17341 gtgatatcac catttactgg ggcgggcgtg aagagcagca tctgtatgat ctctgcgagc
    17401 ttgaggcgct ttcgttgaag catcctggtc tacaagtggt gccggtggtt gaacaaccgg
    17461 aagcgggctg gcgtgggcgt actggcaccg tgttaacggc ggtattgcag gatcatggta
    17521 cgctggcaga gcatgatatc tatattgccg gacgttttga gatggcgaaa attgcccgtg
    17581 acctgttttg cagcgagcgt aatgcgcggg aagatcgcct gtttggcgat gcgtttgcat
    17641 ttatctgaga tataaaaaac ccgcccctga caggcgggaa gaacggcaac taaaacttta
    17701 tacagtggca tttagatcta tgtcgcatcc ggcattcggc acacgctgcc tgatgcgacg
    17761 ctggcgcgtc ttatcaggcc tacaattggt gcaggatcgg tagaccggat aaggcgttca
    17821 cgccgcatct ggcaagtggt taaatccgct caaacaccgt cgcaataccc tgacccagac
    17881 cgatacacat cgtcgcaaga ccaaactggg catccttgcg ctccatgaga tttagcagcg
    17941 tgctgctgat acgcgcaccg gaacagccca acggatgacc cagcgcgatc gcgccaccgt
    18001 tgaggttgat cttctcgtca atctgctcaa tcagtcccag atctttaata catggcagga
    18061 tctgcgcggc aaaggcttca ttcatttcaa acacgccgat atcactgaca gaaagccccg
    18121 cttttttcag cgccaatttt gaggccggaa ccggaccgta acccataatc gacggatcac
    18181 aaccaacgac cgccatcgaa cgcacacggg cgcgcggctt aagacctaat tcatgggcgc
    18241 ggctttcact catcaccagc atggcagctg cgccatcgga aagcgcagaa gatgtacccg
    18301 ccgttaccgt accgctgacg ggatcaaacg ccggacgcag cgtggcgagg gcttccacgg
    18361 tggtttccgg gcgaatcact tcgtcgtaat taaactgcgt caggacgccg tcggcatcgt
    18421 gaccaccggt cgggataatt tcatttttaa atgcacccga ctgcgtggcg gcccaggcgc
    18481 gggcgtgtga ccgcgcggca aaggcatcct gcatttcacg gctgataccg tgcatacgcg
    18541 ccagcatttc tgccgttaag cccatcatgc ccgccgcttt ggcgacatta cggctaaggc
    18601 cggggtgaaa atcgacgccg tgactcattg gcacatggcc catatgctcc acgccgccaa
    18661 ccagacatgt ctgcgcatcg ccgatcatga tcattcgtgc tgcgtcatgc agtgcctgca
    18721 tggatgaacc acacaagcga ttgacagtaa ccgccgggac agagtgcggc acttctgcca
    18781 acagcgccgc gttacgggcg atattaaaac cctgctccag cgtctgctgt acacaacccc
    18841 agtaaatatc gtcgagggcc gccgcttcca gcgccgggtt acgcgccagc aggctacgca
    18901 ttaaatgagc ggagagatct tccgcacgca cgtgacgaaa agcaccgccc ttcgaacggc
    18961 ccatcggggt gcgaattgca tcgacaatga caacctgttc cattgtgact ccttaagcca
    19021 ttttcaggtc gccaaccgga cgggctggct caaccggggg atagtacggt tcgttatgac
    19081 gcgctttatt acgcagacct tccggcactt catacagcgg gccgagatgc tgatattgct
    19141 gttccatatc gaggtatttt gcgctaccga gggtgtccag ccagcggaac gcgccgccgt
    19201 gaaacggagg gaagcccagg ccgtagacca gcgccatatc cgcttctgcc ggagtggcga
    19261 taatgccttc ttccagacag cgcaccactt cgttgaccat cgggatcatc atgcgggcga
    19321 taatctcttc ttcgctgaaa tcgcgcttcg gctggctcac ttctgccagc aggtcgtcaa
    19381 cgacggcatc ttcttctttc ttcggcttac ctttgctgtc ttctttataa cgccagaaac
    19441 cgaggccgtt cttctgacca aagcggttgg cattaaacag cgcgtcgatg gcatcgcgat
    19501 aatctttctg catccgctgc gggaagcctg ctgccatgac agcctgggcg tgatgcgcgg
    19561 tgtcaatgcc cacaacatcc agcagatatg ccggacccat cggccagcca aactgttttt
    19621 ccatcacttt gtcgatcttg cggaaatccg cgccgtcgcg cagcaactgg ctgaaaccgg
    19681 cgaaataggg gaacagtaca cggttgacga agaagccggg gcagtcgtta accacgatcg
    19741 gcgtcttgcc catcttgctc gcccatgcga cgactttcgc gatggtttcg tcggagcttt
    19801 tctcgccgcg aataatttcc accaacggca tccggtgcac cgggttaaag aagtgcatcc
    19861 cgcagaagtt ttccggacgt tccagcgcgt tggccagttc gctgatagga atggttgaag
    19921 tgttagacgc cagcacggta tccgggcgta ctttctgctc ggtttctgcc agtacggctt
    19981 ttttcacttt cgggttttcg acaaccgctt ctaccacaac atccacgcgg tcaaaaccag
    20041 agtaatcgag cgttgggtgg attgtggaga tcacgccagc cagtttcaga ccatcgatct
    20101 tgccgcgctc aagctgcttg ttcagcagtt tcgcggcttc ggtcatgccg agggttaacg
    20161 acttgtcgtt gatatctttc atgacaaccg gtacgccttt ccacgcagac tggtaagcga
    20221 tgccgccacc cataatgcct gcgcccagca cagcggcctg tttcggggtt tcaacgtctt
    20281 tggtgagttt cttcgctttg ccttttacat attgatcgtt aaggaaaatg ccgaccagtg
    20341 cgcgagcttc gttggtatgc gccagcggga caaaactttt gttttccaga tttaatgctt
    20401 cttcacgacc aaaacgggcc gccgcttcaa tggtttttac tgcggtgatg ggggccggat
    20461 aatgtttccc cgccgtttgt gcgaccatcc ctttagcgat ggtgaaactc atggtggctt
    20521 caatcttgct cagttttagc ggttccagct tcggctgacg tttcgctttc cagtcgaggt
    20581 cgccgttaat ggcctggcgt aaaatcgcca tcgccccttc aaccagtttt tctgctttga
    20641 ctacaccatc caccagaccg attttcagcg cctgatccgc gccgacatct ttaccggcgg
    20701 caatgatttc cagcgcactg tcagcgccca gcatacgtgg catacgtaca gaaccgccaa
    20761 agcccggcat gatgcccagt ttggtttccg gcagaccgat gcgcagatcc ggcgtcgcca
    20821 gacgataatc ggtcgccagc acgcattcgc agccaccgcc cagcgcatag ccgttgacgg
    20881 cagcaatggt cggcaccggg agatcttcca ggcgattaaa cacgctattg gcaaagtgca
    20941 gccactgact taactgttct tcaggaacga ggaacaggga caaaaattcg gtgatatcag
    21001 caccgacgat aaaggctgct ttgttcgaac gcagcagcag cccttttaga tctgattgct
    21061 gttccagcac gccgatggcc tcgccgaggc tggcgacggt ggcagtgtcg agtttattaa
    21121 ctgaacctgg ggcatcaaat accagttcgg caatgccatc ttccagccag tcaaggtaca
    21181 gggtgtcgcc tttgtaaagc atgtcagtct cctgaatccg caaggtgatc tggtcgtacc
    21241 agatgcatcg aagtgtgtat tttgtgttaa aaatatgcaa ataaaagatt aaagaaatgc
    21301 cgatctgatc acgctcggca gaaatcacgc tctggatgaa cgatgtgcta agatgcggag
    21361 acttaaggtc aaaaaaacag aagggtaaaa aatggaatca ctggcctcgc tctataaaaa
    21421 tcatatagct accttacagg aacggactcg cgatgcgctg gcgcgcttca agctggatgc
    21481 gttacttatt cactccggcg aactgttcaa cgtttttctc gacgatcatc cctatccgtt
    21541 taaagtgaac ccgcaattca aagcgtgggt gccagtaact caggtgccaa actgctggct
    21601 gctggtggat ggcgtgaata agccgaaact gtggttctat ctaccggttg attactggca
    21661 caacgtcgaa ccgctgccga actccttctg gactgaagat gtagaagtga tcgcgctgcc
    21721 gaaagccgat ggcattggta gtctgttgcc tgctgcgcgc ggcaatatcg gttatatcgg
    21781 tccggtgcca gaacgtgcgc tgcaactggg tattgaggcc agcaatatca acccgaaagg
    21841 ggtgatcgac tacctgcatt actatcgctc cttcaaaacc gagtacgaac tggcctgtat
    21901 gcgtgaagcg cagaaaatgg cggtcaacgg tcatcgcgcg gcagaagaag cgttccgttc
    21961 tggcatgagc gagttcgata tcaacattgc ctatctgacc gcgaccggtc atcgtgatac
    22021 cgacgtacct tatagcaaca ttgtggcgct taacgaacac gctgcggtgt tgcattacac
    22081 caaactggac caccaggcac cggaagagat gcgcagcttc ctgctggatg ccggggccga
    22141 atataacggc tatgcggctg atctaacccg tacctggtcg gcaaaaagcg acaacgacta
    22201 cgcacagctg gtgaaagacg taaatgatga acaactggcg ctgatcgcta ccatgaaagc
    22261 aggcgtcagc tatgtggatt accatatcca gttccatcag cgcatcgcca aactgctgcg
    22321 taaacatcaa atcatcaccg atatgagtga agaagcgatg gtcgaaaacg atctcaccgg
    22381 accgtttatg ccgcacggta tcggccatcc gctgggcctg caggtgcatg acgtagccgg
    22441 ttttatgcag gatgatagcg gtacgcacct cgcggcaccg gcaaaatatc cgtacctgcg
    22501 ctgcacccgt attctccagc cgggcatggt gttaaccatc gaaccgggta tctacttcat
    22561 cgaatcgctt ctggcaccgt ggcgtgaagg gcagttcagc aaacacttca actggcagaa
    22621 aattgaagca ctgaaaccgt tcggcggcat tcgtatcgaa gacaacgtgg tgattcacga
    22681 aaacaacgtg gaaaacatta cccgggatct gaaactggcg tgatggaaag ctggttaatt
    22741 cctgcggcac cggtcacggt cgttgaagag atcaaaaaga gccgcttcat tacgctgttg
    22801 gcgcataccg atggcgttga ggcggcaaaa gcgtttgttg aatcggtgcg ggcagagcac
    22861 cccgatgccc gccaccattg cgtggcgtgg gtggcgggtg cgccggatga ttctcaacag
    22921 ctgggtttct ctgacgacgg ggagccggcg ggaacggcag gtaaaccgat gctcgcccaa
    22981 ctaatgggca gcggcgtcgg ggaaattacc gctgtggtgg tgcgctacta cggcggcata
    23041 ttgcttggca ccggtgggtt agtgaaagcg tatggcggcg gcgtgaatca ggcgctgcgc
    23101 cagctaacga cccaacgcaa gacaccatta accgaatata ctttgcaatg tgaatatagt
    23161 cagttaaccg gcattgaagc gttgctgggg cagtgtgacg gcaaaattat taacagtgat
    23221 tatcaggcat tcgttctgct gcgggtggcg cttccggcgg cgaaagtggc tgaattttcc
    23281 gcaaagctgg cggattttag ccgtggttca ttgcaattgt tagcgattga agaataatcc
    23341 ccacttcgtt ttgcagaact aaggaagcgg cagagatgca ttttcgcgcc attacccgaa
    23401 tcgttggact actggtcatc ttattttcag ggaccatgat tatccctggg ctggtagcac
    23461 tcatctaccg ggatggagcg ggccgcgctt ttacccagac cttttttgtc gccctcgcca
    23521 ttggctctat gctgtggtgg ccgaaccgca aagagaaggg cgaactgaaa tcccgtgagg
    23581 ggtttctgat agtggtgctg ttctggaccg tgctgggtag cgtcggtgcg ctccctttta
    23641 tcttctcgga aagcccgaac ctcacgatta ccgatgcgtt ttttgaatct ttctctggcc
    23701 tgaccaccac gggggccact acgctagtgg ggctggattc gctccctcac gccatcctct
    23761 tttatcgcca gatgctgcaa tggtttggcg ggatggggat catcgtgtta gcggttgcga
    23821 tactgcctat cctcggcgtg ggtgggatgc agctctatcg cgcagaaatg cccggcccgc
    23881 tgaaagataa caaaatgcgc ccgcgaattg cggaaacggc gaaaaccctg tggttgattt
    23941 atgtcttgct gaccgtcgcc tgtgcgctgg cgttgtggtt tgccggaatg gatgcctttg
    24001 atgccatcgg ccatagcttt gcgactatcg ctattggcgg cttctcgaca catgatgcca
    24061 gtatcggtta tttcgacagc ccgactatta acactatcat tgctatcttc ctgctgatct
    24121 ccggctgtaa ctacggtctg cacttttcac tgttaagtgg gcgtagtctg aaggtttatt
    24181 ggcgcgatcc ggaatttcgc atgtttatcg gcgtacagtt tacgctggtg gttatttgta
    24241 cactcgtact gtggtttcat aatgtctaca gttcggcgct gatgacaatt aaccaggcgt
    24301 ttttccaggt ggtatcgatg gcgacaaccg ccgggtttac gactgacagc attgcccgct
    24361 ggccgctctt tttgccggta ctgctcttat gttcagcttt tatcggcggt tgcgccgggt
    24421 caacgggcgg tggcctgaaa gtgatccgca tcctgctgct gtttaagcag gggaaccgtg
    24481 agctgaaacg actggtgcat ccgaacgccg tctatagcat taagctgggg aatcgcgcac
    24541 tgccggaacg tatcctcgaa gccgtgtggg gatttttctc cgcctatgca ttggtgttta
    24601 ttgtcagtat gctggcgatt atcgccacgg gcgtggatga cttttctgcc tttgcctcgg
    24661 ttgttgcgac attgaataac ctggggccag ggcttggtgt ggttgctgat aactttacca
    24721 gtatgaaccc ggtggctaaa tggatcctga ttgccaacat gctgtttggt cgtctcgagg
    24781 tctttacatt gctggtgctc tttaccccga ctttctggcg tgaatgatgg agtaatacgt
    24841 gaaaacatta attcttttct caacaaggga cggacaaacg cgcgagattg cctcctacct
    24901 ggcttcggaa ctgaaagaac tggggatcca ggcggatgtc gccaatgtgc accgcattga
    24961 agaaccacag tgggaaaact acgaccgtgt ggtcattggt gcttctattc gctatggtca
    25021 ctaccactct gcgttccagg agtttgtcaa aaagcatgcg acgcgactga attcgatgcc
    25081 gagcgccttt tactccgtaa acctggtggc gcgtaaaccg gagaagcgta ctccacagac
    25141 caacagctac gcgcggaagt ttctgatgag ttcacaatgg cgtcccgatc gctgtgccgt
    25201 cattgccggg gcgctgcgtt acccacgtta tcgctggtac gaccgtttta tgatcaagct
    25261 gattatgaag atgtcaggcg gtgaaacgga tacgcgcaaa gaagttgtct ataccgattg
    25321 ggagcaggtg gcgaatttcg cccgagaaat cgcccattta accgacaaac cgacgctgaa
    25381 ataagcataa agaataaaaa atg
//
LOCUS       DAZGHF010000071        24816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGHF010000071 DAZGHF010000000
VERSION     DAZGHF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.071"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..75
                     /locus_tag="ACHK3A_002914"
     CDS             <1..75
                     /locus_tag="ACHK3A_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740970.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
     gene            336..677
                     /gene="raiA"
                     /locus_tag="ACHK3A_002915"
     CDS             336..677
                     /gene="raiA"
                     /locus_tag="ACHK3A_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO8740971.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    780..905
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            781..828
                     /gene="pheL"
                     /locus_tag="ACHK3A_002916"
     CDS             781..828
                     /gene="pheL"
                     /locus_tag="ACHK3A_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO8740972.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            927..2087
                     /gene="pheA"
                     /locus_tag="ACHK3A_002917"
     CDS             927..2087
                     /gene="pheA"
                     /locus_tag="ACHK3A_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO8740973.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPQWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGDITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2130..3251)
                     /gene="tyrA"
                     /locus_tag="ACHK3A_002918"
     CDS             complement(2130..3251)
                     /gene="tyrA"
                     /locus_tag="ACHK3A_002918"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO8740974.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAAEIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRH"
     gene            complement(3262..4332)
                     /gene="aroF"
                     /locus_tag="ACHK3A_002919"
     CDS             complement(3262..4332)
                     /gene="aroF"
                     /locus_tag="ACHK3A_002919"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO8740975.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4542..4907
                     /gene="yfiL"
                     /locus_tag="ACHK3A_002920"
     CDS             4542..4907
                     /gene="yfiL"
                     /locus_tag="ACHK3A_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO8740976.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     NAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            5057..5575
                     /gene="yfiR"
                     /locus_tag="ACHK3A_002921"
     CDS             5057..5575
                     /gene="yfiR"
                     /locus_tag="ACHK3A_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO8740977.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5565..6791
                     /gene="dgcN"
                     /locus_tag="ACHK3A_002922"
     CDS             5565..6791
                     /gene="dgcN"
                     /locus_tag="ACHK3A_002922"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO8740978.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNRPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            6807..7289
                     /gene="yfiB"
                     /locus_tag="ACHK3A_002923"
     CDS             6807..7289
                     /gene="yfiB"
                     /locus_tag="ACHK3A_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8740979.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     ALSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7366..7713)
                     /gene="rplS"
                     /locus_tag="ACHK3A_002924"
     CDS             complement(7366..7713)
                     /gene="rplS"
                     /locus_tag="ACHK3A_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO8740980.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7755..8522)
                     /gene="trmD"
                     /locus_tag="ACHK3A_002925"
     CDS             complement(7755..8522)
                     /gene="trmD"
                     /locus_tag="ACHK3A_002925"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO8740981.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8553..9101)
                     /gene="rimM"
                     /locus_tag="ACHK3A_002926"
     CDS             complement(8553..9101)
                     /gene="rimM"
                     /locus_tag="ACHK3A_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO8740982.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9120..9368)
                     /gene="rpsP"
                     /locus_tag="ACHK3A_002927"
     CDS             complement(9120..9368)
                     /gene="rpsP"
                     /locus_tag="ACHK3A_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO8740983.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9505..10866)
                     /gene="ffh"
                     /locus_tag="ACHK3A_002928"
     CDS             complement(9505..10866)
                     /gene="ffh"
                     /locus_tag="ACHK3A_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO8740984.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            11033..11824
                     /gene="ypjD"
                     /locus_tag="ACHK3A_002929"
     CDS             11033..11824
                     /gene="ypjD"
                     /locus_tag="ACHK3A_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO8740985.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11845..13131
                     /gene="yfjD"
                     /locus_tag="ACHK3A_002930"
     CDS             11845..13131
                     /gene="yfjD"
                     /locus_tag="ACHK3A_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO8740986.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13185..13778)
                     /gene="grpE"
                     /locus_tag="ACHK3A_002931"
     CDS             complement(13185..13778)
                     /gene="grpE"
                     /locus_tag="ACHK3A_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO8740987.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13901..14779
                     /gene="nadK"
                     /locus_tag="ACHK3A_002932"
     CDS             13901..14779
                     /gene="nadK"
                     /locus_tag="ACHK3A_002932"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO8740988.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14865..16526
                     /gene="recN"
                     /locus_tag="ACHK3A_002933"
     CDS             14865..16526
                     /gene="recN"
                     /locus_tag="ACHK3A_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO8740989.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKHLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELALLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16675..17016
                     /gene="bamE"
                     /locus_tag="ACHK3A_002934"
     CDS             16675..17016
                     /gene="bamE"
                     /locus_tag="ACHK3A_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO8740990.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(17078..17368)
                     /gene="yfjF"
                     /locus_tag="ACHK3A_002935"
     CDS             complement(17078..17368)
                     /gene="yfjF"
                     /locus_tag="ACHK3A_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO8740991.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17358..17795)
                     /gene="ratA"
                     /locus_tag="ACHK3A_002936"
     CDS             complement(17358..17795)
                     /gene="ratA"
                     /locus_tag="ACHK3A_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO8740992.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17966..18448
                     /gene="smpB"
                     /locus_tag="ACHK3A_002937"
     CDS             17966..18448
                     /gene="smpB"
                     /locus_tag="ACHK3A_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO8740993.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18663..19025
                     /gene="ssrA"
                     /locus_tag="ACHK3A_002938"
     tmRNA           18663..19025
                     /gene="ssrA"
                     /locus_tag="ACHK3A_002938"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(19186..23760)
                     /gene="ypjA"
                     /locus_tag="ACHK3A_002939"
     CDS             complement(19186..23760)
                     /gene="ypjA"
                     /locus_tag="ACHK3A_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO8740994.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQKVDERGTTNNTHIINHGQQNVYGGISNGSLIESGGYQDVGGHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLDSKSTAKNTQIYSGGTQIVDYTSTSDVIEIYSGGVLDVRGGM
                     ATNVTQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTNISSGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVMAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATDANIESGEQIVDGGSTDKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLTNGGVLTVESDTTSAKTQVNA
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSIAGYLATNVLLENGGNLLV
                     LAGTEARDSTVGNGGAMQNLGQDFATKVNSGGQYTLGRSKDEFQVLARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     SELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPKPDPKPDPKPDP
                     KPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNN
                     VWGTTYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHTHIFFD
                     RGGHGSVGSYSLGGYASWEHESGFYLDGIVKLNRFESNVAGKMSSGGAANGSYRSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLS
                     YNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSS
                     SLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(23825..23944)
                     /locus_tag="ACHK3A_002940"
     CDS             complement(23825..23944)
                     /locus_tag="ACHK3A_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO8740995.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(24099..24365)
                     /locus_tag="ACHK3A_002941"
     CDS             complement(24099..24365)
                     /locus_tag="ACHK3A_002941"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8740996.1"
                     /translation="MLRVTTSAHNRGSRPGQNKRLRTLSVGDALLVCKLNRLGRSMWH
                     LVVLLEELCERGINFRALAQSIFAQQWGDGSCKSKTICDLRVIV"
     gene            complement(24696..>24816)
                     /locus_tag="ACHK3A_002942"
     CDS             complement(24696..>24816)
                     /locus_tag="ACHK3A_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8740997.1"
                     /translation="LITGVAPFDGISSMDDDFDDSSSEDEPVENSPVVTSPLV"
BASE COUNT         6260 a         6539 c         5710 g         6307 t
ORIGIN      
        1 gccttcccga gagtggtaaa ggagaggccc tggaaatacc ccacagcccc gaaaaagagc
       61 cagtcagttg cttaactgat cggcattaca gcccttgagc tgccgttttt ttattctgtt
      121 agttgtgaaa ctgaagcgat ttagtcacta tcgatctcat caaatatggc tcgctttgag
      181 atattcctca agtaaaaaaa cacctcttcc tgcgatttct cacaaaaaag attcgttgac
      241 aaaaagtgac aaaattatga gatttccatc acacattttg acatcaggaa cggtatgctg
      301 aattcaccaa gacgggaaga caagaggtaa aatttatgac aatgaacatt accagcaaac
      361 aaatggaaat tactccggcc atccgccaac atgtcgcaga ccgtctcgcc aaactggaaa
      421 aatggcaaac acatctgatt aatccacata tcattctgtc caaagagcca caagggtttg
      481 ttgctgacgc cacaatcaat acacctaacg gcgttctggt tgccagtggt aaacatgaag
      541 atatgtacac cgcaattaac gaattgatca acaagctgga acggcagctc aataaactgc
      601 agcacaaagg cgaagcacgt cgtgccgcaa catcggtgaa agacgccaac ttcgtcgaag
      661 aagttgaaga agagtagtcc tttatattga gtgtatcgcc aacgcgcctt cgggcgcgtt
      721 ttttgttgac agcgtgaaaa cagtacgggt actgtactaa agtcacttaa ggaaacaaac
      781 atgaaacaca caccgttttt cttcgcattc ttttttacct tcccctgaat gggaggcgtt
      841 tcgtcgtgtg aaacagaatg cgaagacgaa caataaggcc tcccaaatcg gggggccttt
      901 tttattgata acaaaaaggc aacactatga catcggaaaa cccgttactg gcgctgcgag
      961 agaaaatcag cgcgctggat gaaaaattat tagcattact ggcagagcga cgcgaactgg
     1021 ccgtcgaggt gggaaaagcc aaactgctct cgcatcgccc ggtacgtgat attgatcgtg
     1081 aacgcgattt gctggaaaga ttaattacgc tcggtaaagc gcaccatctg gacgcccatt
     1141 acattactcg cctgttccag ctcatcattg aagattccgt attaactcag caggctttgc
     1201 tccagcaaca tctcaataaa attaatccgc actcagcacg catcgctttt ctcggcccca
     1261 aaggttctta ttcccatctt gcggcgcgcc agtatgctgc ccgtcacttt gagcaattca
     1321 ttgaaagtgg ctgcgccaaa tttgccgata tttttaatca ggtggaaacc ggccaggccg
     1381 actatgccgt cgtaccgatt gaaaatacca gctccggtgc cataaatgac gtttacgatc
     1441 tgctgcaaca taccagtttg tcgattgttg gcgagatgac gttaactatc gaccattgtt
     1501 tgttggtctc cggcactact gatttatcca ccatcaatac ggtctacagc catccgcagc
     1561 cattccagca atgcagcaaa ttccttaatc gttatccgca atggaagatt gaatataccg
     1621 aaagtacgtc tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt
     1681 tgggaagcga agctggcggc actttgtacg gtttgcaggt actggagcgt attgaagcga
     1741 atcagcgaca aaacttcacc cgatttgtgg tgttggcacg taaagccatt aacgtgtctg
     1801 accaggttcc agcgaaaacg acgttgttaa tggcgaccgg gcaacaagct ggtgcgctgg
     1861 ttgaagcgtt gctggtactg cgcaaccaca atctgattat gacccgtctg gagtcacgcc
     1921 cgattcacgg taatccatgg gaagagatgt tttatctgga tattcaggcc aatcttgaat
     1981 cagcggaaat gcaaaaagca ttgaaagagt taggggacat cacccgttcg atgaaggtat
     2041 tgggctgtta cccaagtgag aacgtagtgc ctgttgatcc aacctgatga aaaggtgccg
     2101 gatgatgtga atcacccggc actggattat tagtgacggt tgtcattcgc ctgacgcaat
     2161 aacacgcggc tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg
     2221 cggaaactgt caataaacgc ctgcttatcg ccctgctcca gcaactcaat cgcctcgccg
     2281 aaacgcttat agtaacgttt gattaacgcc agattacgct ctgacgacat aataatgtcg
     2341 gcatacagtt gcggatcctg ggcaaacagt cgcccaacca tcgccagctc aaggcggtaa
     2401 atcggcgaag aaagcgccag aagttgctca agctgaacat tttcttctgc caggtgcagc
     2461 ccataagcaa aagtagcaaa gtggcgcagt gcctgaataa acgccatatt ctgatcgtgc
     2521 tcgacagcgc taatacgatg cagccgagcg ccccagacct gaatttgctc cagaaaccat
     2581 tggtatgctt ccggcttacg tccatcacac cagaccacaa cttgttttgc caggctaccg
     2641 ctgtccgggc cgaacatcgg gtgtaacccc agcaccggac catcatgcgc cgccagcatg
     2701 gcctgtaatg gtccattttt caccgatgcc agatcgacca gaatacaatc tttcggtaaa
     2761 ggcggtaatt tgccaataac ttgttcagta acgtggatgg gcacactaac aatcaccatt
     2821 ccggcatcgg caactatctc agccgctcga tcccagtcat gttgctccag aatccgcacc
     2881 tgataacccg atagtgtcag catcttctcg aacaggcgtc ccatctgacc gccaccgccg
     2941 acgataacca ccgggcgcag tgacggacaa agcgttttaa atcctttgtc gttttcactg
     3001 gagtaagatt cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg cggtacaccc
     3061 agagcttccg cctctgcacg acgcgaggcc aacatagatg cctcgcgctc cggaacataa
     3121 ataggcagtc caaagcggct tttcacctcg cccacttcag caaccagttc cagacgcttc
     3181 gctaataaat tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa tgcggtcaat
     3241 tcagcaacca taataaacct cttaagccac gcgagccgtc agctgcccgt tcagatcctg
     3301 atgaatttca cgcagcaagg catcggtcat ttcccagcta atgcaggcat cggttacgga
     3361 tacaccgtat ttcatttcac tgcgcggttg ctcggaagac tgattgccct cgtggatatt
     3421 actttcgatc atcagaccaa taattgagcg attgccatct ttgatttgag caaccacgga
     3481 ttctgccacc gcaggctgac ggcgataatc tttattggaa ttaccgtggc tgcaatctac
     3541 catcagagac gggcgcagtc ccgcctgttc catctctttt tcacattgcg caacatccgc
     3601 agggctatag ttcggcgctt taccaccgcg caggatcaca tgcccgtccg gattcccctg
     3661 agtttgtagc aacgccacct gccctgcctg gttaatgcca acaaaacggt gcggctgggc
     3721 ggcggcacgc atagcgttaa tagccgttgc aaggctgccg tcggtgccgt ttttaaaacc
     3781 aaccggcatg gaaagcccgg aggccatttc acggtgtgtt tgcgattccg ttgtacgagc
     3841 accaattgct gaccagctaa acagatcgcc caggtattgc gggctattcg gatctaacgc
     3901 ttccgtcgcc agtggcagtc ccatattcac cagctcaagc agcaatttac gcgcgatctg
     3961 cagcccggct tctacatcaa aagagccatc catatgggga tcgttaatca accctttcca
     4021 accgacagtg gtacggggtt tttcaaaata gacgcgcatt accagataga ggctatcgct
     4081 gacctctgcg gcaagggctt taaatcgacg agcatattcc agagcagttt ccggatcatg
     4141 aatggaacaa ggaccacata ctaccaacag acgaggatcg cgcccggcga taatatctga
     4201 aatggtttta cgcgagtcag caatctgggc ttcctgttgc aggcttaatg gaaaagcggc
     4261 cttcagttgt tccggagtca ttaaaacttg ttcgtcggta atatgtacgt tattcagcgc
     4321 gtctttttgc atgatggcga tcctgtttat gctcgtttgc gatagttgat cctcagcgag
     4381 gatgacgtaa cgataacaca taaagtaaag ttttcaatcc atatttcgta catttttctt
     4441 tacacaagca atttattcgc gcttttaacc cttgtttatg tataaataaa tttacaccac
     4501 ctttgaaaac aatccgctat gctttgaaaa aggagaaaga aatgatgaaa aagtttatcg
     4561 cccccttgtt ggctttactg gttagcggat gtcagattga tccttatact cacgcgccaa
     4621 ccttgaccag caccgactgg tatgatgttg gtatggaaga tgcgatatcg ggcaacgcca
     4681 taaaagatga cgatgcattt agcgattcac aggcggatcg cggtctatac cttaaaggat
     4741 atgccgaagg acaaaagaaa acttgccaga ccgattttac ttatgcccga ggactttccg
     4801 gtaaaagctt tcctgccagc tgcaacaatg ttgaaaatgc cagccaactg catgaagtat
     4861 ggcaaaaagg ggctgatgaa aatgccagca caatacgatt aaattaatta aacccccaca
     4921 aataaattaa acatgagatt ctactcattg tgttttattt ctcacattga tgacgaccgc
     4981 atgaaagaat gatattttaa tgagcattct gaaaaaatat cgatgcattt cgagcgaaga
     5041 tggtgaggat ccctgaatgc gtttttctca ccgactgttc ttactgctga ttctcctgct
     5101 gacgggagca ccaatactcg ctcaggaacc ttctgacgtt gcgaaaaacg tgcgtatgat
     5161 ggtttccggt attgtcagtt acacccgctg gcctgcgcta tcagggccac caaagttatg
     5221 tatcttttca tcttcacatt ttagtaacgc cctacaagag aatggcacga catctttgcc
     5281 ttatctacct gtcatcattc acactcaaca agaggcgatg atttcaggct gcaatggttt
     5341 ttattttggc aacgagtcac cgacattcca gatggaatta accgaacaat atccgtcaaa
     5401 ggcgttatta ttaattgccg aacaaaatac cgagtgcatt attggtagcg ccttttgtct
     5461 aatcatccac aataatgacg tcagatttgc cgtaaacctg gatgccttat cgcgtagcgg
     5521 tgtaaaggtc aacccggatg tcttaatgct cgcacggaag aaaaatgatg gataacgata
     5581 attctcttaa taagcgcccc acgtttaaaa gagcattacg caacatcagt atgaccagca
     5641 tatttatcac tatgatgctg atctggttgc tgctttccgt gacctcggtg ctgaccctga
     5701 aacagtacgc gcaaaaaaac ctggcactga cagcagcaac aatgacttac agtctggaag
     5761 cagctgtcgt ttttgccgat ggccctgcag caactgaaac actggcagcg ctgggccagc
     5821 aaggccaatt ttcaactgca gaagtgcgtg ataagcagca aaatattctg gcatcctggc
     5881 attacaccca taaggaacca ggcgatactt tcagcaattt cataagccac tggctcttcc
     5941 ctgcccccat cattcagccg attcgtcaca atggtgaaac cattggcgaa gtacgcttaa
     6001 ctgctcgcga cagttcaatc agccatttta tctggttttc gctcgctgtc ctgaccggtt
     6061 gtattctgct ggcatcaggc atcgcaatta ccctcacccg ccatttgcac aatggcctgg
     6121 tggaagcgct gaaaaatatc accgatgtcg tacatgatgt gcgttctaac cgcaattttt
     6181 cccgacgagt ttcggaagaa cgtatcgctg agtttcaccg cttcgctctc gacttcaaca
     6241 gtctgctgga tgaaatggaa gagtggcagc ttcgtttaca ggccaaaaat gcgcagcttt
     6301 tacgtaccgc gctacatgac ccattaaccg ggctggctaa ccgcgcagcg tttcgtagcg
     6361 gcatcaacac gttgatgaac aattccgatg cccgaaaaac gtcggcgtta ctatttcttg
     6421 atggcgataa tttcaaatat atcaatgata cctggggtca tgcgacgggc gatagagtct
     6481 tgattgaaat cgcaaaacgg ttagctgaat ttggcggact gcgacataaa gcataccgcc
     6541 tgggcggcga tgaattcgcg atggtgctct atgatgtaca gtcggaatct gaagtgcagc
     6601 agatatgctc agcactgaca caaatcttta atcgcccgtt tgatcttcat aatggtcatc
     6661 agaccaccat gacattaagc attggttacg cgatgaccat tgagcatgcc tctgcggaaa
     6721 aattacaaga gctggccgat cacaatatgt atcaggccaa acaccagcgt gccgaaaagc
     6781 tggtgagata acaaggatat atcgatatga taaagcacct ggtagcaccc ctgattttca
     6841 cctcactaat actgactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg
     6901 ccgctatgca atcttatgga tttactgaat ccgccggcga ctggtcgctg ggcttatcag
     6961 atgccattct gttcgcaaaa aatgactaca aattgctccc ggaaagccag caacagatcc
     7021 aaaccatggc agccaaattg gcctcgacag ggctaacgca tgcccgtatg gatggacaca
     7081 ccgacaacta tggtgaagac agttacaacg aagccttatc attgaaacgg gcgaatgtcg
     7141 tggccgatgc atgggctatg ggtggacaaa ttccgcgcag caatctcacc acacagggtt
     7201 taggaaaaaa atatcccata gccagtaaca agaccgccca gggccgcgcc gagaaccgcc
     7261 gcgtcgcagt ggtgattact accccttaaa acacgagcaa acagataaaa aaagggccag
     7321 ccaattggcc agcccttctt aacaggatgt cgcttaagcg aaatcttagt taagacgctc
     7381 tttgatacga gcagccttac cagtacgctc acgcaggtag tacagtttag ctttacgaac
     7441 agcaccacga cgtttgacag aaatgctgtc aactaccgga gagtgagtct ggaagacacg
     7501 ctcaacgcct tcgccgttgg aaattttacg aacagtgaat gcagagtgca gaccgcggtt
     7561 acgaatagcg ataaccacgc cctcgaatgc ctgcagacgt tttttggaac cttcaacaac
     7621 ccatactttc acttccacgg tatcacccgg acggaaggaa ggtacgtcct gcttcatctg
     7681 ctcttgttca agttgcttaa taatgttgct cataatttaa tctcttatcc tgggtaaact
     7741 gatatctcgg gggcttacgc catcccatca tgtttatgtt gctgttgtgc gtgttccgtt
     7801 ttgaactccg ccagcaacct tgcttgctct tcagtcagag ccaggttttc cagaagttca
     7861 ggtcttctaa gccaggtacg gcccagcgac tgtttcaaac gccagcgacg tatctcggca
     7921 tggttgcccg acagtaacac tggcggaact tccatccctt ctaacacctc aggccgcgta
     7981 tagtgcgggc aatccagcaa tccttcagca aaggaatctt ccgttgccga ggcttcatgt
     8041 cccagtactc ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc tggtaactca
     8101 ccaccactga gaacgtaatc gccgattgac cattcttcgt caatttcggt ttggatcacg
     8161 cgctcatcta taccttcgta gcgaccgcac accagaatca atttttgatt cgttgccagt
     8221 tcgctgacgc ccgcttgatc aagcttgcgt ccctgtggtg acagataaat cacctttgcg
     8281 ccttcacccg ccgcggcttt tgctgcatga atggcgtccc gcaagggttg caccatcatt
     8341 aacatccccg gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg gtcatgcgtg
     8401 aagtcgcgag gactccagct ctggatgctc agcaggccat ttttaactgc ccggccagtt
     8461 accccgtaat cggtaattgc gcggaacatt tcaggaaaca ggctaattat gccaatccac
     8521 atagcgccgt cttttaccgt ttatccggtg gtttaaaaac caggatccca atctacttcg
     8581 attgaacgag tagtgagatc gactttcttg ataacctgcc catcgaggaa cggtacgaga
     8641 cgttccttga taccaaacgc atctttcagg tttgccttaa tgacgagaac gtcattagat
     8701 ccggtttcca tcatatcgac gactttaccg agatcgtagc cttcagtggt tactacctgg
     8761 cagcccatca agtctttcca gtagtagtca ccctcttcaa gctgaggcaa ctgcgatgaa
     8821 tccacgacaa tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc aacgcctttc
     8881 agcttgatga tcatgtcctg attgtggtgc ttccagcttt ccagctggac ttgctgccac
     8941 tgacccgcct tctggataaa ccagggctga tagtcaaaaa tgctttcggc gtcttcggtg
     9001 gaagaaaaca ctctgagcca cccacgaata ccgtaagacg aacccatttt tcccaaaacg
     9061 atgggatcaa caggtgcttg cgcggtgagt tgtttgctca tcatgaccac cgtgacagat
     9121 taagctgctt tgtttacttc tttgatcagc gcagcaacgc gatcagaaat agttgcgccc
     9181 tggccaaccc agtgagcgat gcgatccaga tccaggcgag tgccttcttc tttttcgcta
     9241 gcgattgggt tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc attacggctg
     9301 tcagcgacaa caacctggta gaacggacgc tttttagcgc cgtgacgtgc taaacgaata
     9361 gttaccataa catcctcttg tgtgaataaa acaaccggac cccatcgagg aacggagtcc
     9421 ggtgtcatat taaaagcccg aaaattttac tgatttctgg agaaaatgca atcagcagtt
     9481 aataactctg tcgcataagg tcatttagcg accaggaaat cccggcggca tcataccctt
     9541 catgcttctc atcatcttcg ccattccgcc cttcttcatt ttcttcatca tgcgctgcat
     9601 gtcgtcgaac tgtttcagaa gacggttaac gtcctgcacc tgcataccgc aaccggcagc
     9661 gatacggcgt ttacgcgaac ctttgatgat ttctggctta gcgcgctctt tcatcgtcat
     9721 cgagttgatg atggcttcca tacgcaccag cactttatcg tccatctgcg acttgacgtt
     9781 atccgggatc tgccccatgc ccggcagttt gcccatcagg ctggccatgc cgcccatatt
     9841 tttcatctgg cgcagttgct caaggaagtc attgagatcg aagccgtcgc cttttttcag
     9901 cttgctggct aatttctcag cttgcgcgcg gtcaactttg ctttcgatat cttcgatcag
     9961 cgacagtacg tcgcccatgc cgagaatacg ggaagcgatg cggtccggat ggaacggctc
    10021 cagcgcctcg gttttctcgc caacaccgag gaacttgatc ggtttgcccg taatgtgacg
    10081 aatagagagc gccgcaccgc cacgagcatc gccgtcgact ttagtcagta ccacaccggt
    10141 cagcggtaac gcttcattga atgcttttgc cgtattggcc gcatcctgac cagtcatggc
    10201 atcaaccaca aacagggttt caaccggatt aatcgacgca tggacttgtt tgatctcgtc
    10261 catcatcgct tcgtcaacgt gcagacgacc ggctgtatcc accagcagca cgtcgtagaa
    10321 tttcagtttg gcttctttca gcgccgcgtt aacgatatct accggcttct gaccaacatc
    10381 agaagggaag aaatcaacgc ccacctgctc tgccagcgtc tcaagctgtt tgattgccgc
    10441 cggacgataa acgtcggcag aaaccaccag cactttcttc ttgtgcttct cgcgcaggaa
    10501 cttaccgagt ttaccaacgc tggttgtttt accggcacct tgcaggcccg ccatcagcac
    10561 gaccgcaggc ggttgcgcag ccaggttcag ggtctggttc tcttcgccca tcgccgcaac
    10621 cagttcgtta cggacaattt tgacgaactc ctgccccggc gtcaggctct tattaacttc
    10681 atgaccaacc gctttctctt ttacgcgatt gataaactca cgcactaccg gcagagcgac
    10741 gtccgcctcc agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt
    10801 gaggcgtcca cggccactga tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc
    10861 aaacattgtc tctcgcctgg ggtggaaacg gttggccgca atcgcgacac atcatcagta
    10921 ttttgccgca gtataacatg aaggcgtctt tgttgttatg caacggttgg agcagcgttc
    10981 acctgacgct atactgcttc tctttcttat tgctcaaact gtcgacatta ctatgcccgt
    11041 ttttgctctg ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg ttcccggtct
    11101 gctgcaaaaa aacggcggct ggcggcgcat ggctattatt tctgcggtca ttgcgctggt
    11161 ctgccacgca atcgctctgg aagcccgcat cctgcccgac ggtgatagcg gacaaaacct
    11221 cagcctgctg aacgttggtt cattggtcag tttgatgatc tgtacggtaa tgaccattgt
    11281 ggcttctcgc aatcgtggct ggttgctgct acccattgtc tatgcctttg cgcttatcaa
    11341 cctggcgctg gcgaccttca tgcccaatga atacatcacc catctggaag caacgcctgg
    11401 gatgctggtg cacattggct tatcgctctt ttcctatgcc acgctgatta tcgccgccct
    11461 gtacgcgctg caactggcgt ggattgatta ccaactgaag aacaagaagc tggcgtttaa
    11521 ccaggaaatg ccgccattga tgagtatcga gcgtaaaatg ttccacatca cgcagattgg
    11581 cgtggtgctg ctaacgctca cgctttgcac tggcctgttc tacatgcaca acttatttag
    11641 catggaaaat atcgacaagg ccgtgctctc tatcgtggcg tggtttgtct atattgtgct
    11701 gctgtgggga cattatcatg aaggatggcg tggacgccgc gtcgtctggt ttaacgttgc
    11761 gggtgcagtc attctgacac tggcctactt cggcagccga attgtccagc agttaatcag
    11821 ctaaacccag aaaaggagtt tcccctggaa cacatttcta ctactacgtt gatcattatt
    11881 ctgatcatca tggtggtcat ttcagcctat ttttccgggt ccgaaaccgg aatgatgacc
    11941 ctcaaccgct atcgtctgcg acatatggcg aaacagggta atcgctcggc caaacgcgtc
    12001 gaaaaattgc tgcgtaagcc agaccgcctg attagcctgg tgttaatcgg caataacctg
    12061 gtcaatattc ttgcctccgc gctaggcact attgttggga tgcgtttgta cggcgatgcg
    12121 ggcgtggcaa ttgcgactgg tgtgctgact tttgtcgtgc tggtttttgc tgaggtattg
    12181 ccgaaaacca ttgccgcgct gtacccggaa aaagtcgctt atccgagtag ttttctgctg
    12241 gctccgctgc aaattttgat gatgccgctg gtctggttgc tgaatgctat cacccgtatg
    12301 ctgatgcgca tgatgggtat caaaaccgat atcgtggtta gcggctcttt gagcaaagaa
    12361 gagttgcgca ctatcgtgca cgaatcgcgc tcacaaattt cccgtcgcaa tcaggatatg
    12421 ctgctgtcgg tgctcgatct ggaaaaaatg accgttgatg acatcatggt gccgcgcagt
    12481 gaaattatcg gtattgatat caacgatgac tggaaatcga ttctgcgcca actctcccac
    12541 tcacctcacg ggcgcatcgt gctctaccgt gattcgctgg acgacgccat cagtatgctg
    12601 cgtgtgcgtg aagcctggcg actcatgtcg gagaaaaaag agttcaccaa agaaaccatg
    12661 ctacgcgccg cggacgagat ctattttgtc ccggaaggta cgccgctcag cacgcagttg
    12721 gtgaagtttc agcgcaacaa aaagaaagtc ggcctggtcg tcaacgagta tggagacatt
    12781 caggggctgg tgacggttga agatattctg gaagagattg tcggcgattt cactacctcg
    12841 atgtcgccaa cacttgccga agaggtcacg ccgcaaaacg acggttcggt gattatcgat
    12901 ggcaccgcca acgtgcgaga aatcaacaaa gcctttaact ggcatctacc tgaagacgat
    12961 gcccgcacgg ttaatggcgt cattctggaa gcgctggaag agatccctgt cgctggcacc
    13021 cgcgtgcgta ttggcgagta cgatatcgat attctcgacg tacaggataa tatgattaag
    13081 caggtaaaag tttttcctgt gaaaccgctg cgcgagagtg tggcggagta aaaaaacggc
    13141 ccggcattcg catgctgggc cgtgatttat tacgaaagca gaaattaagc ttttgccttc
    13201 gctacagtca ccatcgccgc acgaatcgta cgaccgttca gcgtataacc tttctgcata
    13261 atgcccagta cgttacctgg cgcaacgtca tcagattcca ccattgcgat ggcctgatgc
    13321 acatttgggt ccagtgggac gttggtttcg gcgatcactt caacgccaaa cttacgcaca
    13381 acatccagca tcgacttcag cgtcagctca ataccttcaa ccatcgcgga catatccggg
    13441 ttagctttat cagccacttc cagcgcacga tccaggctat caatcaccgg cagcaattcg
    13501 ttgatgaatt tctccagcgc gaatttgtga gctttttcaa tatccagttc agtacgacga
    13561 cgcaggtttt ccatttcggc ttttacgcgc aaaatgccgt cacgttcacg ggtctgggct
    13621 tcagccagct gagcttcgag attcgcaatt ttttcatcgc gcggatccac ctgctcagca
    13681 gaagcttctg gctcaactgc ctcaatctct tcgtgctgat ccatgataat ttcttccggg
    13741 gcttgcccct caggcgtttt ctgttcttta ctactcatga atttctccgc gtttttttcg
    13801 cattcatctc gctaacttcg cttattatgg ggatcagttt cagggtttca agggaagcac
    13861 tcacattgtc atcaatcttc gcaacaagga cctcggaaaa atgaataatc atttcaagtg
    13921 tattggcatt gtgggacacc cacggcaccc cactgcactg acaacacatg aaatgctcta
    13981 ccgctggctg tgcacaaaag gttacgaggt catcgttgag caacaaatcg ctcacgaact
    14041 gcaactgaag aatgtgaaaa ctggcacgct cgcggagatt gggcaactgg cagatctcgc
    14101 ggtagtcgtt ggtggcgacg gtaatatgct gggcgcggcg cgcacactcg cccgttacga
    14161 tattaaagtt attggaatca accgtggcaa cctgggtttc ctgactgacc ttgaccccga
    14221 taacgcccag caacaattag ccgatgtgct ggaaggccac tacatcagcg agaaacgttt
    14281 tttgctggaa gcgcaagttt gtcagcaaga ttgccagaaa cgcatcagca ccgcgattaa
    14341 cgaagtggta cttcatcctg gcaaagtggc gcatatgatt gagttcgaag tgtatatcga
    14401 cgagatcttt gcgttttcgc agcgttccga tggactgatt atttcgacgc caacaggctc
    14461 caccgcatat tccctctctg cgggcggtcc tattctgacc ccctctctgg atgcgattac
    14521 cctggtgccc atgttcccgc atacgttgtc agcacgacca ctggtgataa acagcagcag
    14581 cacgatccgt ctgcgttttt cgcatcgccg taacgacctg gaaatcagtt gcgacagcca
    14641 gatagcactg ccgattcagg aaggtgaaga tgtcctgatt cgtcgctgtg attaccatct
    14701 gaatctgatt catccgaaag attacagtta tttcaataca ttaagcacca agctcggctg
    14761 gtcaaaaaaa ttattctaat tttacgccag cctctttact gtatataaaa ccagtttata
    14821 ctgtacacaa taacagtaat ggtttttcat acaggaaaac aactatgttg gcacaactga
    14881 ccatcagcaa ctttgctatc gttcgtgagc ttgagattga ttttcatagc ggcatgaccg
    14941 taataactgg cgagaccggc gcgggtaaat ctattgcaat agatgccctc ggcctttgtc
    15001 tcggtggtcg cgctgaagcc gacatggtgc gtaccggcgc tgctcgtgcc gacctgtgcg
    15061 cccgtttttc tctgaaagat acgccagcgg ccctgcgctg gctggaagaa aaccagcttg
    15121 aagacggtca tgaatgtttg cttcgtcgcg taatcagtag cgatggtcgc tcacgaggtt
    15181 ttatcaacgg tacagctgtt cctctgtcac aacttcgcga actgggccag ttgctgattc
    15241 agatccatgg tcagcacgcc catcagttac tcaccaaacc cgagcaccaa aaattcctgc
    15301 ttgatggcta tgccaatgaa acctctctgc tccaggaaat gaccgcacgt tatcagttgt
    15361 ggcatcaaag ctgccgtgac ctcgcgcatc atcaacagtt aagtcaggaa cgcgccgccc
    15421 gtgcggaact gctgcaatac caattaaaag aacttaacga atttaatccg cagcccggag
    15481 agtttgagca aatcgacgaa gagtacaaac atctggcgaa cagcggtcaa ttgctgacta
    15541 ccagccagaa tgcattggca gtaatggccg acggtgaaga cgcaaacctg caaagtcagc
    15601 tttacacggc taaacaactg gtgagcgaat tgattggcat ggacagcaaa ctgtccggcg
    15661 tacttgatat gctggaagaa gctaccatcc agattgctga agccagcgat gaactgcgcc
    15721 actactgcga tcgtctggat ctcgatccca accgactgtt tgaacttgaa cagcgcatct
    15781 caaaacagat ttcgctggca cgtaaacatc acgtcagccc tgaggcattg ccacagtatt
    15841 accagtcgct actggaagaa cagcagcaac tggacgatca ggccgactca caagaaacgc
    15901 tcgcgctggc ggtaacgaaa catcatcagc aggcactgga aaccgcgcgc gcattacacc
    15961 agcaacgcca gcagtatgca gaagaacttg cactgctgat caccgacagt atgcatgcgc
    16021 tctcaatgcc gcatgggcag tttacgatcg atgttaaatt tgacgagcat cacctgggcg
    16081 ctgacggtgc cgatcgtatt gagtttcggg taaccaccaa cccaggtcag ccaatgcagc
    16141 ctattgccaa agtcgcatcc ggtggtgaat tgtcacgcat cgcattggca attcaggtca
    16201 tcacggcgcg taaaatggaa accccggcac tgatttttga tgaagtggat gtagggatta
    16261 gcggtccaac agcggcagtt gtcggcaaac tgctgcgtca acttggcgaa tcaactcagg
    16321 tgatgtgtgt tacccacctg ccacaagtcg cgggatgtgg tcatcaacac tattttgtca
    16381 gcaaagaaac cgatggtgcg atgacagaaa cacacatgca atccctggat aaaaaagcgc
    16441 ggttacaaga gctggcgcgc ctgcttgggg gcagtgaagt cacacgtaac acactggcga
    16501 atgcgaaaga actgcttgcg gcgtaaactt ttttcctgct tcacggtcag agtaaacagc
    16561 aaaacgccgt aagaccggaa agcaaaaggt tttaaagtga tgaaaggtct attatcatcg
    16621 gcatattaca gatgagccac gtactgctcg ggcccgaaaa ggaatcaaat cactatgcgc
    16681 tgtaaaacgc tgactgctgc agcagcagta ctattgatgt tgaccgcagg ctgttccact
    16741 ctggagcgag tggtttaccg tcctgacatc aaccagggga attatctgac cgctaacgac
    16801 gtatccaaaa tacgcgttgg catgacgcaa caacaagttg cgtacgcatt gggtacaccg
    16861 ctgatgtccg atccatttgg tacgaatacc tggttctatg tcttccgcca gcaaccaggt
    16921 catgaaggtg taacgcagca aacgctgacg ctgaccttta atagtagcgg ggtgttgacc
    16981 aatattgata ataaacctgc gctgagtggt aactaatcat aaagttgctc tcaaagacgt
    17041 taaaaaaggt gctcaatgag cacctttttt ctgtctgtta tttattcgct gatttttctg
    17101 ctcgttgcct gcgcagctct ttcggatcgg caatgagagg gcgataaatc tccacccgat
    17161 cgccatcatg cacactatcg ctcagttttg ccggacggct ataaatgccg actttgtttt
    17221 tagttaaatc gatatcggta cgcaattcca gcaagccact ggcgcgaata gcttcttcaa
    17281 ccgtcgcacc ctcctgcagc gtcacacgct gcaggtactg cttctcaggt agcgcataag
    17341 ccacctcaac ggcaatttta cctggcactg taaacctctt tcgcacgaac cgtaaaagcc
    17401 tggaccatat tagccgccag ttctttaaac acgcggccaa aggcgagttc aatcaactta
    17461 ttggtaaact caaaatcgag atgaaactcg atacggcacg cctcctgact cagcggcgta
    17521 aacttccatc caccgatcaa tttcttgaac ggcccatcca ccagattcat aagaatactt
    17581 tggttactgg tcaactggtt gcgggtagta aacgttttgc tgatcccagc cttagagaca
    17641 tctaccgccg cagtcatctg cccaggagtg gactccagaa tccgacttcc ggtacaaccc
    17701 ggcaaaaact gaggataaga ctgaacgtca ttcactaact gatacatttg ctccgcgctg
    17761 tagggtacca gtgcggtccg gctaatctga ggcataacaa tttccatcaa caaaaatcca
    17821 acaaataata tcatttatcc tgctgtaaaa aaaacgctat cccggcgctg ggtaacatcg
    17881 ggttcatgct aagatagagc cttgtccccc gcaggattga tatggggtgt tttcgatttc
    17941 agattaccga tgattcacga cgcttatgac gaagaaaaaa gcacataaac ctggttcagc
    18001 gaccatcgcg cttaacaagc gcgcccggca cgaatacttt atcgaagaag agttcgaggc
    18061 gggacttgcc ctgcaaggct gggaggttaa atccctgcgt gcaggaaaag ccaatatcag
    18121 cgacagctac gtccttctgc gtgacggaga ggcatttctg tttggcgcta acatcacgcc
    18181 aatggctgtg gcctccacgc atgtggtgtg cgatcctacc cgtacccgca agttacttct
    18241 caaccagcgc gaactggact cattgtacgg tcgcgtcaat cgagaaggct ataccgtagt
    18301 ggcgctctcc ctgtactgga aaaatgcctg gtgcaaagtg aaaatcggcg tcgccaaagg
    18361 taagaaacag catgataaac gttcagatat caaagaacgc gaatggcagg tggataaagc
    18421 gcgtatcatg aaaaacgccc accgttaaac ctgcactgca attattgatc cgctcctcac
    18481 cgcgcctccc tctccggcgg cgcgaatgca catcttattg gctatcacat ccgacccaaa
    18541 tgttgccatc ccattgctta atcgaataaa aatcaggcta catgggtgct aaatctttaa
    18601 cgataacgcc attgaggctg gtcatggcac tcatgaatct ggtatactta ccgttacaca
    18661 ttggggctga ttctggattc gacgggattt gcgaaaccca aggtgcatgc cgaggggcgg
    18721 ttggcctcgt aaaaagccgc aaaaaatagt cgcaaacgac gaaaactacg ctttagcagc
    18781 ttaataacct gcttagagcc ctctctccct agcctccgct cttaggacgg ggatcaagag
    18841 aggtcaaacc caaaagagat cgcgtggaag ccctgcctgg ggttgaagcg ttaaaactta
    18901 atcaggctag tttgttagtg gcgtgtccgt ccgcagctgg caagcgaatg taaagactga
    18961 ctaagcatgt agtaccgagg acgtaggaat ttcggacgcg ggttcaactc ccgccagctc
    19021 caccaatcat gattggacgg tgtaaggaca acaccaacaa aaacaggaag ttagcagtct
    19081 cagcaagaca ccgaccagac ggtgaggaga cataaaagga tacgcaaagg agccgcggct
    19141 cctggtgata tgaaagccca cagatgtggg ctttttcgtt gatggtcaga acgaccagtt
    19201 cacaccagcc accgcgttcc acggggattc cacaccggca ccatggctat accccacccc
    19261 gagatgcccg cttaacgaac tgctgaatga ggctttaata cctgcctggt atattccacg
    19321 tctgcccgac aaatcattga caaaattacc gtcattattc actttcaccc ggttatcatc
    19381 gacaaattct ttgcgcacag ccgccttcag ccacggctca acttccatgc cgttccccag
    19441 acgcatgttg taactcagcg ttgcgcccag ttcacgatat atactacggg tatcgactga
    19501 tttcgattcc atgccattgg ataaatgata ttcggggtta tcagcggtga accccgttaa
    19561 cgatgcatac ggcgtcaggt tccagttacc atcggtaaat cgcatcccgg tttcaatgtg
    19621 accgcccagc ccgttgctgc gataactgcc gttggcggct ccaccgctgc tcattttacc
    19681 ggctacgtta ctttcaaagc ggttcagctt cacgataccg tccagataga aaccactttc
    19741 atgttcccag ctggcatagc cccccagaga ataactgccc acactgccat gccctccacg
    19801 atcaaaaaag atatgtgtat gggaataacc cataaaagcg cccagcgtcg caatcccctc
    19861 aggaatatca ttacggctat cgatccccac cgtcattccg gtcagcgtct gctcaaaccc
    19921 ggcacccgca tcggtggtga cattattacg ggtgttatac gtcgtccccc agacattatt
    19981 gttgtgtgga ctcgctttca ttatgttcaa ccgctcgcga atactgttta gctcagcatc
    20041 aaagaccaac ggtaatgttg ctgccatatt gagtacggct gccgtagaag gcgtgatgcg
    20101 tttctccggg acgggtgtcg gcgttggatc gggagtcggg tctggtttcg gatccggttt
    20161 tgggtctggt tttggatccg gcttcggatt tgggttgggg tccgggttgg gtttgacatc
    20221 attggtcagg ttccagttgc tgttgccatc gcttttcagg acatactcat aggtcccaag
    20281 atcaacgaaa ccgccggtat tacccagcgt aaacgaagca tcccctcccc ctgttttcac
    20341 cagcgtcatc gcgtcgtcag actgagggct gacgccggta tcctgaacaa agattttaaa
    20401 attgccagtg gcgttgttgt tgacgaccag ttgatcgccc cgggagcctg caacgttggt
    20461 atgcaggtag aaattaccgc tgccggaaag ttcactggtg gtcagcgtat tgtagatacc
    20521 attagttgtg gcaggcgctg ctgtttgtgc tgataaataa acatcaccgt cgttgaggag
    20581 caaactattt actctatatt cgcagttgct ggtgcctgca caggaattat tgctgttaag
    20641 ccagacactg ccccggctgg cagccgtcag gtcggcaagc gtcgtatcga caccatttcc
    20701 cagagttaat gtcgcgctat cggtaatccg gaccgcccct tcaagtttta ctggcgtaac
    20761 attatcccgt ggcgtcatta acgacaggct tcctgtcgcg ccactgaccg atgcatccgc
    20821 cagcgtacct gcgtagacga ttgccgtccc gccagcaacc tgtagatctt ctgcccgggc
    20881 cagaacctga aactcatctt ttgaccgacc gagggtatat tgcccaccag aattaacctt
    20941 tgtggcgaag tcctgcccca ggttttgcat tgccccaccg ttgccaaccg tggaatcgcg
    21001 agcttcggta cctgctaata ccaataaatt accgccattt tccagcagta cattggtcgc
    21061 taaatatccg gcaatggaaa aggtgccgta ctggtgcgta ccgctgattt caataccgtt
    21121 agccgtgctc gtctggagag cggcaccgct gttctggacg atatctgtcg ctttgccatt
    21181 atcgttgact gtcagcgttc cgccttcatt gatcgttgtt tttattgcct ctccgttagc
    21241 tgaaactgtt tgtattccgc cgtcgttaat tgttgtctca tttgccacac cctcgacgat
    21301 ttgttcaccg ccagtgagcg tcgtgccagt cgcagtggct tttgttttga cgatctcccg
    21361 accgcccgca ttgacctgtg ttttggcgga agtggtgtct gactccacgg ttaacacgcc
    21421 gccatttgtc agcaggatat tgttcgccgc accctgctcg atgctgaacg cgacgccatc
    21481 agcgcgtgtc cctgtgaccc gcgtcgccct ggtggttgca accaacgcgc cctgactact
    21541 ctgctgtatc cccgttgcgc tgcctttctc ccgcacatcg agtgtgccgc cgtcattaag
    21601 caccgagttt tcagccagac cgccctcatt aactacctgt gaaccgccat taataatgga
    21661 accttccgct gtcccgtttg ccataatttg ttgtaagcca gagacgatat cggtattgat
    21721 cgccttacca taattctgaa cggtttgtgt gccgccattg atgtgcgttt tgtctgttga
    21781 cccaccatca acaatttgtt caccactttc aatatttgca tcagtggcta aaccatatac
    21841 cgtttgcttg ccacctttga tatttgcttt atcagaagtg gcactggcat atattgtttg
    21901 ggtgcccgca ctattgagta cagtgccaac atcttttcca taaacatcca ttttgccgtt
    21961 ggcattaata atcgtatcaa ctgcccggga accagtgacg actgttaatg agccagcgtt
    22021 ttccagcact acatttttag cttctgaatt cctgatgtag aaagcatcac cataactgtt
    22081 ggttccttcg ataagggttc cggaagtcgt ggaagcaatt aatgcgccgc cggattgttg
    22141 ctcaacatgc ttagcctcac caccgtcctg aacctccaga acgccaccat tattaagtct
    22201 ggtgctatct gttttagcct ccttctggac aatcagctta ccgccagcat caatggtagt
    22261 atttttcgcc gaggttttag ccattaccgt cagttcgccg gtattttcca gcacaacata
    22321 attagcctca cctccggtaa tagtgaagtg agagagctta ttgtatcctt cgatatcagt
    22381 ccctgcgccc gtgttggcaa ctaaagcact gcccgtctcc tggttaaccc catgtgcaat
    22441 accgccggta tagacaatca gcgagcctcc ggcgctaata ttgctgccag ttgccgtacc
    22501 atctttctca acaacctgcc ggctcccaga ggatatattt gtcgtgtcag ctttcccgcc
    22561 gctcttaata ttttgtgttc cgctgttgat attggtgccc gtggcaatac cataattatt
    22621 aatattctgt gtgccaccat taattatggt atttgtcgcg tttccggcaa catccataac
    22681 gccgccatta tctattcggg tcgcatcagc tttagcattc gttaaaactg acattgttcc
    22741 tttatcttta ataatcgtct tatttgccga accatatgcg tttatgtcta aatgaccacc
    22801 gttttccagc aacacattgt ctgccacgtt attgtggatg gagaatgccc cttcactatt
    22861 cgtaccgctc accgtcaaat cgtaagtcgt tactttcaaa gcggctccat cgtgctgagt
    22921 aacatttgtt gccataccac ctctaacatc aagcacgcca ccggaataaa tttcaataac
    22981 atccgaggtg ctagtgtaat caacaatttg cgtaccacca gaatagatct gagtattttt
    23041 tgccgttgac ttactatcca gagactgagt tccaccttca atcgtcgtgt ccagcgcacg
    23101 gctctcatat actctttgct caccgccatt tttaatggtg gttgtttcta ctgtgctctg
    23161 ttcaacatac tgtcgaccac catttatggt tgtgttcgtc gccagacttc cttgcgtgat
    23221 gtcctgataa ccggacttat ttatcgttgt accatcaaca tgcccctgag ttgttactgt
    23281 ctgccctcca ccatcaatga tggttccatt cgcagtcccc ccacttatgt gtgaattacc
    23341 acccttaatt gtcgttccat tgctgatacc acctgtataa acgtcctgat tgccactctc
    23401 gattattgta cctgtggaaa tacccccgtc atgaattgac tgtctgcccc cgttaatggt
    23461 tgtattatta gcctgcccca caaaattgtt atgacctcct acatcttgat atccacccga
    23521 ttcaataaga cttccattag atatcccgcc ataaacattc tgctggccat ggttgataat
    23581 atgagtgtta tttgttgtac ctcgttcatc tactttttgg ctgccatcta cagtctcatc
    23641 gtttaccaca ccaataacat caggagtgaa ggccgccatc ccgggcgggg catatatcaa
    23701 ggcagatatc aataaggaaa gtactgagcg acgacaataa tagggactgg tcctgttcat
    23761 aaatttcatc ctctgaaaag tgaatactga gtagcgttta agcgacctta gctttgctgc
    23821 aacatcagcc cacaggcacc agaccagggg attcatcctg aagagacagc gcaagtgtat
    23881 tgtgttcacc gctcatcaaa gacatcatga tgaaatgatg atattccgca taagaatgag
    23941 gcatttttta aacgcagtgc gctgaagtgt ggttggataa aaaagtcaat ctattcagga
    24001 aacacgggcg tattcttttc tttcgacaat caggcagtcg gaaaaataaa atgatttaca
    24061 taatcgtttc tgatgaatat cttctgctca cataaaaatc acacaataac tctgagatcg
    24121 cagattgttt tacttttaca gcttccgtcc ccccattgtt gggcaaatat agattgggcc
    24181 agagcacgaa agttaatacc acgttcgcac agctcctcca acagcacgac aagatgccac
    24241 atactgcgcc ccagtcggtt cagtttacag actagcagtg cgtcccctac cgataatgtc
    24301 ctgagccgtt tgttctgtcc gggcctgctg ccacggttat gcgctgaagt ggtaactctg
    24361 agcacgcagt tcttcagtca ggcgtggtgc accgtcaccc tattattgct gggtaagatc
    24421 aaaaaacttt caagcagcta aggaaagttg aaccagacat tagatgaaat atttcaacca
    24481 attacagcac cagttcagac accgccaact catcaaataa atcaaggggt tacgtgaaag
    24541 cgtagcccct tttactttgg tagtgacagc aaaatggatg cagtgtgaag aataatcccg
    24601 tttactcaat caataataca tattatttca atctacgtta ttatttcttt gtaaaaatcg
    24661 ccatttattt atcattgaaa actgctttta gaactttata caaggggact agtcacaaca
    24721 ggactattct caacgggctc atcctcagag gaactatcat caaagtcatc atccatagat
    24781 gaaataccat cgaatggtgc cacgcccgtg attagt
//
LOCUS       DAZGHF010000072        24558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGHF010000072 DAZGHF010000000
VERSION     DAZGHF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.072"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            219..989
                     /gene="yjjP"
                     /locus_tag="ACHK3A_002943"
     CDS             219..989
                     /gene="yjjP"
                     /locus_tag="ACHK3A_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO8740998.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            980..1453
                     /gene="yjjB"
                     /locus_tag="ACHK3A_002944"
     CDS             980..1453
                     /gene="yjjB"
                     /locus_tag="ACHK3A_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO8740999.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1561..2100
                     /gene="dnaT"
                     /locus_tag="ACHK3A_002945"
     CDS             1561..2100
                     /gene="dnaT"
                     /locus_tag="ACHK3A_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO8741000.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2103..2840
                     /gene="dnaC"
                     /locus_tag="ACHK3A_002946"
     CDS             2103..2840
                     /gene="dnaC"
                     /locus_tag="ACHK3A_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO8741001.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2889..3383
                     /gene="yjjA"
                     /locus_tag="ACHK3A_002947"
     CDS             2889..3383
                     /gene="yjjA"
                     /locus_tag="ACHK3A_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO8741002.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3637..5928
                     /gene="opgB"
                     /locus_tag="ACHK3A_002948"
     CDS             3637..5928
                     /gene="opgB"
                     /locus_tag="ACHK3A_002948"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO8741003.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6066..7088)
                     /gene="lgoD"
                     /locus_tag="ACHK3A_002949"
     CDS             complement(6066..7088)
                     /gene="lgoD"
                     /locus_tag="ACHK3A_002949"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO8741004.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGALVAVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7227..8141
                     /gene="lgoR"
                     /locus_tag="ACHK3A_002950"
     CDS             7227..8141
                     /gene="lgoR"
                     /locus_tag="ACHK3A_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO8741005.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRDIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8356..9717
                     /gene="lgoT"
                     /locus_tag="ACHK3A_002951"
     CDS             8356..9717
                     /gene="lgoT"
                     /locus_tag="ACHK3A_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8741006.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMIGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9766..11430)
                     /gene="tsr"
                     /locus_tag="ACHK3A_002952"
     CDS             complement(9766..11430)
                     /gene="tsr"
                     /locus_tag="ACHK3A_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO8741007.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAASEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11612..12475
                     /locus_tag="ACHK3A_002953"
     CDS             11612..12475
                     /locus_tag="ACHK3A_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO8741008.1"
                     /translation="MAYSIDIISCITERFMALTATEKRIAQFILDDVSAAVELPIAEL
                     ARLTQTSQASVTRFARALGCKDVRELKVKLAQSLAVGQRFILDVPDLDGVQGIYEAII
                     SVLETHRRSLDMNAISQAVSWLSQARQILALGTGGGSTICSQEIQYRLFRLGLPVVSQ
                     NDALMMRMMCSAVMPQDVVIVLSLGGYTPEIIESAAIARQYGARVIAITPAQTPLAEQ
                     VDLVLPLLVQESDYIFKPTPSRYAMLAMVDVLATELAMANKAQAKDRLRRIKLVLDSH
                     RGGANRQPLGD"
     gene            complement(12485..13954)
                     /locus_tag="ACHK3A_002954"
     CDS             complement(12485..13954)
                     /locus_tag="ACHK3A_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFO8741009.1"
                     /translation="MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLF
                     LTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVGGAVGILLVAWLFAPVRKLRFMT
                     MSEELSYYTGGSHLIKNIVGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIA
                     LAFAIYVIIGGYSAVVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSL
                     FAIDKMGVLPALSLALVIGVGVLATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSIL
                     PAIIGMAAFTMNPNLENSNYAFLFATSFLPAILGLVVLIAGLSATMSSASSDAIAAVA
                     IMMRDVYTMITGRMPPEDKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMS
                     GLFICSILGRFWLRFNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVA
                     GVLVTLVTPASQVSREEALAIITNERENQSVVITKPEEA"
     gene            complement(14057..14446)
                     /locus_tag="ACHK3A_002955"
     CDS             complement(14057..14446)
                     /locus_tag="ACHK3A_002955"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO8741010.1"
                     /translation="MSIKRYGTEGGTGTGGQHLPFARAVEAGGWLYVSGQTPMKNGEV
                     VEGGIVEQSRLAIQNCVDIMTEAGYSLADVVHVKVILTDARYFQSFNKVFREFFGENP
                     PARICCVADLVVDCKVEVDVTCYNATR"
     gene            complement(14456..15898)
                     /locus_tag="ACHK3A_002956"
     CDS             complement(14456..15898)
                     /locus_tag="ACHK3A_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO8741011.1"
                     /translation="MQVDWLIKNVTVIDGSGGPEFRGDVAITGDRIVDIAPALNVTAQ
                     QVIDGEGRVLAPGFIDVHTHDDINVIRIPEYLPKISQGITTVIVGNCGISAASAKMKG
                     EVPDPMNLLGEAEHFIYPTVESYAQAVEAARPSLNVGTLIGHTALRNNHMDDLFRPAT
                     VDEIAAMRADLRLALSQGALGLSSGLAYATAFQATTEEVMALAEELAGEKGVYTTHLR
                     SEFEPILDALDEAFRIGRHGKVPVVVSHHKCAGAKNWGRTKETLAFFDQMRQHQEIGC
                     DVYPYSASSSTLDLKQVTDEFDIVITWSQTHPQQAGKTLAQIAIDWQMSMLEAAKLLM
                     PAGAIYYNMDERDVRRVLSYPVSMIGSDGLPNDPMPHPRLWGAFPRVLGHYCRDEGLF
                     PLTTAIHKMTGLSASRFRLPQRGLVKVGYFADLVLFDPQTIRDVASFSDPKRPADGIE
                     AVMVNGVMSYGPDKHITGRAGRFLRRQTSH"
     gene            16099..17319
                     /locus_tag="ACHK3A_002957"
     CDS             16099..17319
                     /locus_tag="ACHK3A_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HFO8741012.1"
                     /translation="MKYHSEPLVPHKSALMQMPANLLAEDVCLPAAIIKKQALENNIT
                     WMQRYADARGVSLAPHGKTTMTPWIFQAQQAAGAWAIGVGSAWQAGAAMASGIQRVLM
                     VNQLVGKANMQLIAQLQRHYPTVDFICCIDSIENARALSAFFASQQQTLNVMIELGVP
                     GGRCGCRSRDAALTLAKQAAQLPGLRLRGLELYEGVLHGDDPQPQVEALLRDAAQLAC
                     DMASLVDGEFILTGAGTVWYDVVCNIWLAAEKPDNCRIVIRPGCYITHDTGIYDAAQQ
                     QLLARDPVACDLAGDLTSALELVAMVQSVPEADRAVVNFGKRDCAFDAGLPQPVAHYR
                     NGKSLAFDPQAIRSTGIMDQHCMLQLSANSDVQVGDILVFGTSHPCLTFDKWKTLLLT
                     DDDYNVLAELDTLF"
     gene            17587..19737
                     /gene="btsT"
                     /locus_tag="ACHK3A_002958"
     CDS             17587..19737
                     /gene="btsT"
                     /locus_tag="ACHK3A_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO8741013.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            19787..19990
                     /gene="yjiX"
                     /locus_tag="ACHK3A_002959"
     CDS             19787..19990
                     /gene="yjiX"
                     /locus_tag="ACHK3A_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO8741014.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            20001..20957
                     /gene="yjiA"
                     /locus_tag="ACHK3A_002960"
     CDS             20001..20957
                     /gene="yjiA"
                     /locus_tag="ACHK3A_002960"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO8741015.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEVEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPSRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDKIRAAFAGLRK"
     gene            complement(20991..21293)
                     /locus_tag="ACHK3A_002961"
     CDS             complement(20991..21293)
                     /locus_tag="ACHK3A_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFO8741016.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(21308..21409)
                     /locus_tag="ACHK3A_002962"
                     /pseudo
     CDS             complement(21308..21409)
                     /locus_tag="ACHK3A_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            21606..22772
                     /locus_tag="ACHK3A_002963"
     CDS             21606..22772
                     /locus_tag="ACHK3A_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO8741017.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVDREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            22836..24455
                     /locus_tag="ACHK3A_002964"
     CDS             22836..24455
                     /locus_tag="ACHK3A_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO8741018.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEVLGYGE"
BASE COUNT         6016 a         6479 c         6152 g         5911 t
ORIGIN      
        1 aagggctaaa caataaattc attatagtga taatattcat gcgataattt gtttgcgacg
       61 agagataatt cgctttttaa caccgagctt ctcccgataa ccaggattaa tcagcgcaat
      121 tttgatctta acctgccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc
      181 tgaacaatac gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag
      241 ccgtaacacg gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg
      301 cgttggttga tgagctttcc tcacgactgg gtcgggcgtt gggaatggac agcgtcgaaa
      361 gttctatctc ttcgaacgcc atagtgctga caactattaa agatgggcaa tgcctgacat
      421 cgacacgtaa aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca
      481 ttgtgattct ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc
      541 aaattcagcc attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg
      601 cctgtttctg taaactcaat aacggtggct gggatggtgc cgtcatcacc ttctttgcca
      661 gtacggccgc gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccacagatca
      721 acttttgcct caccgctttc gccgccacca ccatttccgg tttgcttttg caacttccca
      781 ctttcagcaa tacccccacc atcgcgatgg ccgccagcgt tctgctgctg gtgccgggct
      841 ttccgttgat taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac
      901 gctgggcgat cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga
      961 cgatttgggg gctacgcgga tgggtgtgat cgaatttctg ttagcgttgg cgcaggatat
     1021 gatcctcgcc gccattcctg cggtcggctt tgcgatggtg ttcaacgttc ccgtgcgggc
     1081 gttacgctgg tgtgcgttgc ttggctcgat aggtcatggt tcccgaatga tcttaatgac
     1141 cagcgggctg aatattgagt ggtcaacttt tatggcttct atgctggtcg gcaccattgg
     1201 tattcaatgg tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggctgttat
     1261 tcctatgttc cctggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca
     1321 gctaggttac agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc
     1381 gattgttggt gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg
     1441 ccctcgcgta taaaattgcc tcacttaaag cacgcggatg agagacagcc tcctctcctc
     1501 cgtgtgttac tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt
     1561 atgtcttcca gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac
     1621 caaaccgttc tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg
     1681 gcgttttatg ccgttacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg
     1741 gcgcgtccgg gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct
     1801 cccgttgctg tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgac
     1861 tttatccgcc tggcggcgct atggggcgtg gcgctaagag agccggtgac caccgaagaa
     1921 ctggcctcat tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg
     1981 caacaaaaac tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa
     2041 cgagatgtga atacggtcag cgaacctgac agccaaattc caccaggatt cagagggtaa
     2101 cgatgaaaaa cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca
     2161 aacctgcgtt caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc
     2221 gctccgccgc tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct
     2281 ccggtattcg tccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag
     2341 ggcagatgaa tgcgttaagc aaagcgcgcc agtatgtcga agagtttgac ggcaacatcg
     2401 ccagcttcat cttttctggc aagcccggaa ccggcaaaaa ccatctggcg gcggcaatct
     2461 gcaacgagct gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt
     2521 cggcgatgaa agataccttc aggaatagcg gtaccagcga agaacaactg cttaacgatc
     2581 tgagcaacgt tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg
     2641 aaaaagtgat catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga
     2701 tgctgaccaa cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc
     2761 gtatgcgcct gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg
     2821 taacgggtaa agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt
     2881 gagtaatgat gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat
     2941 taatttccac cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg
     3001 aacttggcaa ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta
     3061 acttgcttag cagcggaaac caggccttaa gcgcagataa catgaacaat gccgcaggca
     3121 ttctgcaata ctgcgcgaag caaaagctgg cttcggtaac cgacgccgaa aacatcaaga
     3181 atcaggtact ggaaaaactg ggcctgaaca gtgaagagca aaaagaagac accaactatc
     3241 tggatggcat tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataata
     3301 ttggaacgac tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc
     3361 aggggctgaa cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga
     3421 cgcggctctc tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa
     3481 gcgaccgcga tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg
     3541 acactagaat tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac
     3601 gttggctaaa cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc
     3661 ctttttctcg cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt
     3721 gcagccacat taacggtact ggggctattt gtcgttttaa atatcaccct gtttgccagc
     3781 gactatttta ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg
     3841 accggtgctg gtgtcagtaa atacatcctg cctggtatcg gcattgtgct ggggctgaca
     3901 gcggtgttcg gtgcgctggg ctggatcctg cgccgtcgtc gtcatcatcc gcaccatttt
     3961 ggttacagcc tgctggcact cttactggcg ttgggttcag tggatgccag cccggcattt
     4021 cgtcagataa cggaactggt gaaatcccag tcacgcgacg gcgacccgga ctttgcggct
     4081 tattataaag agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac
     4141 ggcgaaagtc tcgagcggac ctattttgat aacgaggctt tcccggatct cacgcctgaa
     4201 ctgggcgcgt tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg
     4261 gattacacga ttgcgggcat ggtggcttct cagtgcggaa taccgctgtt tgcccccttt
     4321 gaaggcaacg cctccgcttc tgtttccagc ttcttcccgc agaacatctg tctgggcgat
     4381 atcctgaaaa actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc
     4441 ggtaaagatg tgttcctgaa gtcgcatggc ttcgaccact tatatggctc agaagagctg
     4501 aaaagcgtgg tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt
     4561 ctcgacgaag cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg
     4621 tttaccctga cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc
     4681 aaaaaatatg attttgacgg taagccgaat cagtcattca gcgcggtaag ttgcagccag
     4741 gagaatatcg cgacgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc
     4801 atcgtcgtct cttctgacca tttagcgatg aacaacacgg cgtggaaata ccttaataaa
     4861 caggatcgca ataacctgtt ttttgtcatc cgtggcgaca agccgcagca agagacgctg
     4921 gcggtgaagc gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat
     4981 aactatctcg gacttggtcg tagcagttta tccgggcagt caatgtcgga aatcttcctc
     5041 aatatcaaag agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct
     5101 aaagagatga aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc
     5161 catttccgtt tgccgctact gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa
     5221 agtgaatact cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc
     5281 gtctgggttg accgttgcta caagatggca caactttggg ctccggaact ggcactctcc
     5341 accgactggt gtgtctcgca agggcagctt ggcggtcagc aaattgtcca acatgttgac
     5401 aaaacaacat ggaagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttac
     5461 aaaggcaatg tcgatacgct gaagattgtt gataacgata ttcgctacaa agccgacagt
     5521 ttcatcttta acgtcgccgg tgcgccagaa gaggtgaaac agtttagcgg gatttcccgt
     5581 ccggagtcgt ggggccgctg gtccaacgct cagctgggcg acgaagtaaa aatcgagtac
     5641 aagcatccgc tgccgaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac
     5701 gccagccgtc ctattccggt acgcgtaggc aatgaagaac aaactcttgt gctgggcaat
     5761 gaagtgacca ccaccacgct gcatttcgat aacccaaccg atgccgacac gctggtaatt
     5821 gtgccgccgg aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag
     5881 ctcgggatcg gcatggtgga aattaaagtg gtagaacgtg aagggtaatt cctgactgat
     5941 gtgtaggtcg cataaggtgc gacctacacg ccttctccgg cttacaacac actattaaga
     6001 aacaaaaaaa acagcgccca acacttcccc ggacgctgca tggcaacttt aataatctcg
     6061 ccttatcaga aagtaattac acccttaatc aactcacgat tgttaatcac atcacgctcg
     6121 taggtttctg ccagcgtggc gaacgggtag cgatgggtta acatcatgtc ggcagtgatt
     6181 ttcccttccg ccatcagtcg accgacttta gcgaaatctt ccggcgtggc gttgcggcta
     6241 cccatcatcg tcgtttcttt tttatggaat tctggatcgg agaactgcaa ctcaccttta
     6301 aacagaccga caaataccac cgtgccgccg tgacgaatca gattcacggt gttattcatc
     6361 gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc aaactgcgcc
     6421 cgcagctgcg cgtcaaaatc ttcggctgat gggtccagca caggcaactc cagacgcgtt
     6481 gccacatgtt cacggcgcgc cggactggta tccgccaccg ctaccagtgc gccatcggct
     6541 ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag cacctgctcg
     6601 ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg ttcaatcaat
     6661 gctgccgcct gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg cacgctcaaa
     6721 tactcactaa aaccgccatc ctgatgcacg ccaatgactg aaattttttc acagcaattg
     6781 gtacgcccgc ttttacacgc cgggcattgc tgacaggcaa cataagggat cacagcaact
     6841 tgctgaccat ttttaagatc agcaatattt ttacccagcc caacaatctc cccacatatt
     6901 tcatggccta aaacgcgtgg ataactaaaa aatggttgat ttccacccca ggcatgaata
     6961 tcggtaccgc aaatcccgac agacttaatt tttattaatg cttcattgtc acccggaata
     7021 ggtatctcgc gttgtttcca gactaattct ttcggctgct ggcaaattaa aacattcatc
     7081 gtagacatga ttcggctctc cattttttgt tgccttagat atcaatgaaa atgaaaaatg
     7141 gcagcggaac ggaaaatctg ttttgtgaaa acacacgcat aaaaatgttt taaatcgggt
     7201 tttaatggac tcacaaggag gaaaatatga gtcgttcaca aaatttacgc cacaatgtga
     7261 ttaaccaggt cattgatgat atggcgcggg gtcatatccc ttccccactt ccatcgcaaa
     7321 gtgcgctggc ggaaatgtac aacatcagcc gcactacagt gcgtcatatt ctcagccact
     7381 tacgcgaatg cggcgtctta acgcaggttg gaaacgacta tgtgattgcg cgtaaacctg
     7441 accatgatga cggtttcgcc tgtactaccg cttcaatgag tgaacaaaac aaagtgtttg
     7501 agcaggcttt tttcacgatg ataaaccagc gccagttacg cccaggggaa actttttctg
     7561 aactgcaact ggctcgggcc gcgggtgtca gcccggttgt agtaagagaa tatcttttaa
     7621 aattcggccg ttataatctt attcaaagcg aaaagcgcgg ccagtggagc atgaagcaat
     7681 tcgatcaatc ctacgctgag cagctgtttg agctacgcga aatgctcgaa acacattcac
     7741 ttcagcactt tctcaacctg cctgatcatg atccccgctg gctgcaggcg aaaaccatgc
     7801 tcgaacgtca tcggttactg cgtgacaaca tcggcaatag ctttcgcatg ttctcgcaac
     7861 ttgacagaga ttttcactcc ttactgcttt cagctgccga caatatcttt tttgatcaat
     7921 cgcttgagat catctcagtc atctttcatt ttcactatca atgggacgaa agcgatctta
     7981 aacagcgcga tatcattgcc gtcgatgagc atatgaccat tctcagtgct ctgatttgcc
     8041 gcagtgacct tgatgccacc cttgcgctcc gtaaccattt gaattcagct aaacaatcaa
     8101 tgattcgatc cattaatgag aacacgcgtt atgctcatta agtacggatt aaaaacagca
     8161 aactattagc gggtgcacgg ttttatcaaa agataaatac cgggaaattc ccgcttacct
     8221 atgctcacaa tcagtcgact aaaaatgttt ttgtgagcgg tagtaaagtc ctaaaacttt
     8281 aacctgccgt taatgtcaac cttgcgtaat gcacaattac gtaaacaatt aaaattataa
     8341 atacaggagt ccggcgtgga aaaagaaaat atcaccctcg atccgcgttc ttcatttact
     8401 ccatcttcgt cggcagatat tcccgtgcca ccagatggat tagttcaacg cagtacccga
     8461 attaaacgca ttcaaaccac cgccatgttg ttattatttt ttgcggcggt aatcaattat
     8521 ctcgaccgca gttcgctgtc ggttgcaaat ttaacgattc gtgaagaatt gggattaagt
     8581 gctaccgaaa tcggcgcttt gctctccgtg ttttcactcg cttacgggat tgcgcaactt
     8641 ccttgcggcc cactattgga tcgtaaaggc ccacgcctga tgctgggact ggggatgttc
     8701 ttctggtcac tgttccaggc catgtctggc atggtgcaca gctttacgca gttcgtgttg
     8761 gtgcgtatcg gtatggggat tggtgaagcg ccgatgaacc catgcggtgt aaaagtcatt
     8821 aacgactggt tcaacatcaa agagcgcgga cgcccgatgg gcttcttcaa cgcagcttct
     8881 actattggcg ttgccgtaag cccaccgatt ctggcggcga tgatgctggt gatgggctgg
     8941 cgtgggatgt ttattaccat tggtgtgctg gggatttttc tcgccattgg ctggtatatg
     9001 ctctatcgca accgcgagca cgtagaactg actgccgttg aacaagctta cctcaatgct
     9061 ggtagcgtca atgcccgccg agatccgctc agttttgccg aatggcgcag cctgttccgt
     9121 aaccgcacaa tgtggggaat gatgatcgga ttcagtggca tcaactacac tgcgtggctg
     9181 tatctggcct ggcttcctgg ttacctgcaa acagcctata acctggattt aaaaagcaca
     9241 gggttgatgg cggctatccc tttcctgttt ggggctgccg ggatgctggt caacggttac
     9301 gttaccgact ggctggtcaa agggggaatg gctccgatta aaagccgtaa gatctgcatt
     9361 attgccggga tgttctgttc tgccgccttt acgctggtcg taccgcaagc gacaacatcc
     9421 atgacagcgg ttctgctgat tggcatggca ctgttttgta ttcactttgc cggaacatcc
     9481 tgctggggct tgatccacgt cgcagttgct tctcgcatga cggcgtcggt gggcagtatc
     9541 cagaactttg ccagcttcat ctgcgcctct ttcgcgccga tcattactgg ttttattgtt
     9601 gataccaccc actcattccg tctggcacta atcatctgcg gttgcgtcac cgcagcgggg
     9661 gcactggcgt acatcttcct ggttcgtcag ccgatcaacg acccgcgtaa agattaaact
     9721 ccctatgccc gataacattt tgcttatcgg gcatttttat ggcgattaaa acgtttccca
     9781 attctcgccg ctatctgcca cggccatttt acgcggcgta gctggcgtca cggtttttac
     9841 tacagccgat gtttcacgct gctgttgctg ctgttgctga atccggaaca ctgccactgc
     9901 ttcggtcagg cgactggcct gctcttccag cgcggcggcg gcagcggcag attcttccac
     9961 cagcgcggcg ttttgttggg ttacccggtc catctcagca accgctaagc caacctgatc
    10021 gataccacgg ctctgctcat cagaagcaga agcaatttcg cccataatgt ccgtcacgcg
    10081 ggtcacggca ctgacaatct ccgccattgt ttccccggcg ctttcgacca gcgtagagcc
    10141 aacatccact ttccccaccg agtcttcaat caggctttta atttcacgag cagcctgagc
    10201 gctacgctgg gccagattac gcacttctcc cgcaaccacc gcaaaaccgc gcccttgctc
    10261 acccgcgcgc gctgcttcaa ccgccgcgtt caaagccaga atattggtct ggaaggcaat
    10321 accgtcaatt acgctgataa tatcggcgat tttctgcgaa ctggtggaga tatcgcgcat
    10381 agtctgcacc acgttatcca ccactttgcc gccgcgttgc gccgtttcgg aagcacttaa
    10441 cgccaggtgg ctggcctgac gcgcattttc ggcgttctgc ttcaccgttg cggtcagttg
    10501 ctccatgctg gctgccgtct cttccagcga agcggcctgt tgctcggtgc gcgaagagag
    10561 atcgttattg ccggtggcga tttcgctggc accgctatag atggcattgg ccccgttgcg
    10621 cacatcaccg acggtacgca tcagctctcc ctgcatatgg cgcaaactct ctgccagttg
    10681 ccccatctca ttagagccat ccacctcaat cggtttcacc agatcgccgc ctgcaatatg
    10741 acgaatgctg tcaatcaggc gattcattgg cgcgaccagc gaggctttaa taccgaacca
    10801 gacggcgagg atgaccgcca gtacgacgat catcacgcct accagaatcc acatcgcctg
    10861 gctgtaggag gcattgttat cgctgacggc gatatcatag agccgatcgt tttgctccat
    10921 gtaagccaca tactgcttct cgaaaccgtc ctgatatccc tgggtcggtt gatcaaagaa
    10981 ctcgttgatt ttgcctgcac ctaacagttg gatcagctcc gccagcgcat tgtgataaat
    11041 atcgtaatta cgtttgatct ctgacgctgc ggcggtgctc tgacgcgggt cacgcggcaa
    11101 cgcttcgtaa tccgcccagt ttttttccgc ctgtttcagc gaaatactgg cactctgcat
    11161 cagctcagca acggttgaac cgctaccaat attattctga tccatcatgt agcggatacc
    11221 cgcgcggttg agggtgttac gcgtctgcaa caacgcaacc cagctgccat tcagcgtgga
    11281 ttgctgctgg cgaatggttt gtaaaacagt gaaattttct ttgtcattct ttaaggcatt
    11341 aaagaacaga ccgcctgatg tcagttgtaa aaggccaaaa acggccaaaa ccagcagtaa
    11401 gctggtcacg atcttgatac gttttaacat ggtttctctt tccactagac agatacactg
    11461 gttgtcggcc tggtggtgga aaactttatg ctgtggaaaa gcgtcggcac tgcttttgtt
    11521 gcagcaaact tgcgtttttt gtgagggtca cactctatag cggcagttta tggtacaaag
    11581 tatcaatctt cttcgaacca gtgatactcc tatggcttat agcatcgata tcatctcctg
    11641 cattaccgaa cggtttatgg cgttaacagc cactgaaaag cgtatcgcac aatttattct
    11701 tgatgacgtc tctgccgcag tagagttgcc aattgctgag ctggcacggt taacgcaaac
    11761 cagtcaggcg tcggtgacgc gttttgcccg tgcactgggg tgcaaagatg tccgcgagtt
    11821 gaaagtcaaa ctggcgcagt cactggctgt cgggcagcgg tttattctcg atgtaccgga
    11881 tcttgacggt gttcagggga tctacgaagc gattattagc gtgctggaga cgcatcgtcg
    11941 ttcgctggat atgaatgcca tcagccaggc tgtaagctgg ttaagtcagg cgcggcaaat
    12001 tcttgcgtta ggaactggcg gcggttccac tatctgttca caggagattc agtaccgttt
    12061 attccgtctg gggctaccgg tggtcagtca gaatgatgcc ctgatgatga gaatgatgtg
    12121 ctcggcagtc atgccgcagg atgtggtgat tgtgctttcg ctgggcggtt atacaccgga
    12181 aatcatcgaa agtgcggcaa tcgcccgtca gtatggcgcg cgtgttattg ccattacgcc
    12241 agcacaaaca ccgctggcgg aacaggtgga tctggtattg ccgttactgg ttcaggaaag
    12301 tgattacatc tttaagccca ccccttcccg ctacgcgatg ttagccatgg tcgatgtact
    12361 ggcaacagaa ctggcaatgg caaataaagc gcaggcgaaa gatcgcctgc ggcggataaa
    12421 actcgtgtta gacagccacc gtgggggcgc taaccgccag ccacttggcg attagcattg
    12481 tcggttatgc ttcttcaggt ttggtgatca cgacactctg gttttcacgc tcattggtga
    12541 taatagccaa agcttcttcc cggctgacct gacttgctgg cgttaccaac gtcaccagta
    12601 cgccagcgac caggctgccc agcactgacg gaatacacgg gttaccccag taagccagcc
    12661 agtcggcatt catcagcacc acaattgaca tcaccatacc gctcactaac gccgccagcg
    12721 cgccctgcca gttaaagcgt agccagaagc gaccgagaat cgagcagata aacagcccgg
    12781 acatcagcat cgaaatcatt ttggtaatgt agctgatgat gtcattagag gtcagagcaa
    12841 agaccagcgc caggccaata acaaaggtca acatccagcg tgagtaagta atggccttgt
    12901 cctccggcgg cattctgccg gtaatcatgg tgtagacgtc gcgcatcata atcgccaccg
    12961 ccgcgatggc atcggagctg gcagaagaca tggtggctga aagcccggca atcagcacca
    13021 ccagcccaag aatggcaggt aagaaactgg tcgcgaacaa gaaggcgtag ttgctgtttt
    13081 ccagatttgg gttcatggtg aacgccgcca tgccgataat ggcaggcaaa atcgagaaga
    13141 acagatataa caccccagta ataacaaacg aacggcgtac agagggaaca tctttacccg
    13201 agtaaatacg ctggcgataa gacggtgttg ccagcacacc tacgccaata accagtgcca
    13261 gcgaaagcgc aggcagcacg cccattttat caatcgcaaa caggctcatc gcttttgggt
    13321 ccatcgcctg ctcaatcgca ctccagccgc caacgtgaac aacggcaaga atagccatta
    13381 atataaagcc aaagaacaga attagggcct gaatagtatc tgtccacact accgccgaat
    13441 aaccgccgat aataacataa atcgcaaaag ctaaggcgat aattaatttt gccacagtaa
    13501 gatcaatacc cgtcgcccag gcaagataca tacttccacc taatatatgc gcccccagcc
    13561 agccaataga ggcaataaat atcatcaggc cgacaatatt tttaattaaa tgactaccgc
    13621 cagtataata agagagttct tcactcatgg tcataaaacg taatttacgt accggtgcaa
    13681 acagccacgc caccagcaat atacctacgg ctccaccgac gccataaagc attccggccc
    13741 agccattgct gtagccaaag cccactgccc ccatactgga acccgtgcca accatcgtgg
    13801 caacggttga ccccagcgtc agaaacagcg gcagcgaacg cccgcccagc agaaaatctt
    13861 ctccgctttt ctgattacga gaaacaaacc aaccaagcca aatcatggcg atggcataaa
    13921 taataaagcc gactaaaaag atatgactgt tcataattca catcctgcca gtagagagaa
    13981 attaatgtaa gagttcccgg agtgctgaat gtattcagca ctcaattgcg gtctgttttt
    14041 ttaattctta aatttattag cgggtcgcgt tataacaggt cacatcgact tcgactttac
    14101 aatcgacaac cagatcagcc acacaacaaa tacgtgcagg cggattctcg ccaaaaaatt
    14161 ctctgaatac tttattaaat gactggaaat agcgggcgtc ggtcaggatc actttcacgt
    14221 gaaccacatc cgccaggcta taaccggctt cagtcatgat gtcgacacag ttttggatcg
    14281 ccagtcgtga ctgttcaacg attccgcctt ctaccacttc accgtttttc atgggtgttt
    14341 gcccggagac gtacagccag ccgcccgcct caaccgccct ggcaaaaggc aaatgctgcc
    14401 cgccagtgcc tgttccacct tccgtaccgt aacgtttaat gctcatattc actccttaat
    14461 gtgaggtctg ccggcgcagg aagcgtcccg cacggcctgt aatatgttta tcgggaccat
    14521 agctcataac gccgttcacc atcacagcct caatgccatc tgccgggcgt ttgggatcag
    14581 aaaagctggc aacatcacga atggtttgtg gatcgaacaa caccaggtcc gcaaaataac
    14641 cgactttcac cagtccacgc tggggcaggc gaaaacggct ggcagaaagc ccggtcattt
    14701 tgtggatagc cgtggtcagt gggaataagc cttcatcacg gcaatagtgg cccagcacgc
    14761 gagggaaggc gccccataaa cgtggatgcg gcatgggatc attgggcagg ccgtcagagc
    14821 caatcatact gaccggataa ctcaacactc ggcggacgtc gcgctcgtcc atgttgtaat
    14881 agatagcccc ggcaggcatt agcagcttcg cggcctccag catactcatc tgccagtcga
    14941 tggcgatttg cgccagtgtt ttcccggcct gctgcggatg tgtttgtgac caggtgatca
    15001 caatatcgaa ttcgtcggtg acttgtttga gatccagcgt tgaagagctg gctgagtaag
    15061 ggtagacatc gcagccaatt tcctggtgtt gacgcatttg gtcaaaaaag gccaacgttt
    15121 cttttgtccg cccccagttt ttcgccccgg cgcatttatg gtgggaaacc acgacaggca
    15181 ctttgccatg acgcccaata cgaaacgcct catccagggc atcgagaatc ggttcaaatt
    15241 cagaacgcag atgggtggta taaacgccct tctcaccggc taattcttcc gccagcgcca
    15301 tgacttcttc ggtagtcgcc tgaaatgccg ttgcataggc cagcccggaa cttaacccca
    15361 gcgccccctg actcaacgcc agacgtaagt cggcacgcat agcggcaatt tcatccaccg
    15421 tcgccgggcg aaacaggtcg tccatatggt tattacgcag cgccgtatga ccaatcagcg
    15481 tgccaacgtt cagtgacggc ctggctgctt ccactgcctg ggcatagctt tcaacggtag
    15541 gataaataaa gtgctctgct tcccccaaca gattcatcgg gtcaggaact tcacctttca
    15601 ttttcgccga cgccgcacta atcccgcagt taccgacaat caccgtagtg atcccctggc
    15661 tgatctttgg cagatattcc gggatgcgaa taacgttgat atcgtcatgg gtatgaacgt
    15721 cgataaaccc gggtgccagc acccgccctt ccccgtcaat gacctgctgc gccgtaacgt
    15781 taagcgcagg ggcaatatcg acaatccgat cacctgttat tgccacgtca ccgcgaaatt
    15841 cagggccgcc gctaccgtcg ataacggtca catttttgat taaccagtca acctgcatgt
    15901 tcgtcactcc atttctgcaa tggtgacaag ttaaccattt acaaggacga aaaacagtgg
    15961 aaaactacgc aaaacccaga tgattttatg atacttttat tcataaataa taagataaat
    16021 aaactaaata tttatatttc atttagttac aaatgatact cacatccgat acggtgaaaa
    16081 acttaaaagg taaatgttat gaaataccac tcagaacctc tggttcccca taaatctgcc
    16141 ctgatgcaaa tgcccgcaaa tctccttgcc gaagatgtct gtttacctgc cgcgatcatt
    16201 aaaaagcagg ccctggagaa caacattacg tggatgcaac gctacgctga cgcgcgcggc
    16261 gtttcactgg caccgcacgg taaaaccacc atgacgccgt ggatttttca ggcgcagcag
    16321 gcggctggtg cctgggcaat aggtgtcggt agcgcatggc aggctggtgc ggcaatggcg
    16381 agcggtattc agcgagtgct gatggttaac cagctggtcg gcaaggcgaa tatgcagttg
    16441 attgcccagt tgcaacgaca ttacccgacg gtcgatttta tctgctgtat cgacagcatt
    16501 gagaacgccc gcgctttgtc tgcctttttt gccagtcagc aacagacact gaacgtgatg
    16561 attgagttgg gtgtccccgg cgggcgctgt ggttgccgct caagggatgc cgcactgaca
    16621 ctggctaaac aggcagccca attaccgggg ttacgcctgc gggggcttga gttatatgaa
    16681 ggcgttttac acggtgacga tccacaaccg caggtagaag ctctgctgcg ggatgccgca
    16741 caactggcct gtgatatggc aagtctggtt gatggcgagt ttattctcac cggagcaggc
    16801 acggtctggt atgacgtggt gtgcaatatc tggctggcgg cagaaaagcc tgataactgc
    16861 cgcattgtta ttcgccctgg ctgctacatc actcacgaca ccgggatcta cgacgcggca
    16921 caacaacagt tgcttgcccg tgatcctgtg gcctgcgatc tggcaggcga tctcacatct
    16981 gcgctggaac tggtcgccat ggtgcaatcg gtgccggaag ctgatcgtgc ggtggtaaat
    17041 tttggtaagc gtgattgcgc cttcgacgcc ggactgccgc aaccagtcgc ccactaccgc
    17101 aacggcaaat cactggcatt tgatccacag gcgattcgca gtacaggtat tatggatcag
    17161 cactgcatgt tgcaactgtc agctaatagc gacgtgcaag tgggggatat tctggtgttt
    17221 ggcacatcgc atccgtgcct gaccttcgac aaatggaaaa cgttgttatt gactgatgac
    17281 gactacaacg tactggcaga attagacact ctcttctaag aacgcccctt cggttaatta
    17341 tcgaaggggc atttttattg tgatctcaat cacatatcca cccgacgaat aattcttgcc
    17401 caaccgctca ccgccattcc tgaccgctta catccctaaa ataaccactc agttatttac
    17461 cttactttac gcgcgcgtaa ctctggcaac atcactgcag gatagcggtc aatttacctc
    17521 ctcaaacgca acgcaaacct agaacggctt cggccaacta ttaatcaata catgccaggt
    17581 tttactatgg atactaaaaa gatattcaag cacataccct gggtgattct cggaatcatc
    17641 ggtgcattct gcctcgcggt agttgcatta cgtcgggggg agcacgtcag tgccctgtgg
    17701 atcgtggtcg cctctgtgtc ggtgtatctg gtggcttatc gctactacag tctgtacatc
    17761 gcccagaagg tgatgaaact cgaccccacg cgcgcgacgc ctgcggttat taacaacgac
    17821 ggtctgaact atgttccgac caaccgttac gtgttgtttg gtcaccactt cgccgctatc
    17881 gccggtgctg gtccgctggt tggtccggtt ctcgccgcgc agatgggcta cctgcctggc
    17941 acgctgtggc tgctggcggg ggtagtgctg gccggtgcgg tacaggactt tatggtgctg
    18001 tttatctcct ctcgccgtaa cggcgcatct ctcggtgaga tgatcaaaga agagatggga
    18061 ccagtaccgg ggactatcgc gctgtttggc tgtttcttaa tcatgatcat catccttgcc
    18121 gtcctggcgc tgattgtggt taaagccctg gccgaaagtc cgtggggtgt cttcaccgtt
    18181 tgctcaaccg taccaattgc gctgtttatg ggtatctaca tgcgctttat ccgtccgggg
    18241 cgtgtgggtg aagtctctgt cattggtatc gtgctgctgg ttgcctctat ctacttcggt
    18301 ggcgtgattg ctcacgatcc gtactggggt ccggcactga cctttaaaga caccaccatt
    18361 accttcgcgc tgattggcta tgcgtttgtt tccgcgctgc tgccggtgtg gctgatcctc
    18421 gcaccacgcg actatctggc aaccttcctg aaaatcggcg ttatcgtcgg cctggcgctg
    18481 ggtatcgtgg tgctgaaccc ggaactgaaa atgcctgcga tgacccagta cattgacggt
    18541 actggcccgc tgtggaaagg cgctctgttc ccgttcctgt tcatcaccat cgcctgtggt
    18601 gcggtatctg gcttccacgc gctgatctct tccggtacga cgccgaaact gctggctaac
    18661 gaaaccgacg cgcgtttcat tggctacggc gcaatgctga tggagtcctt cgtggcgatt
    18721 atggcgctgg ttgctgcgtc catcatcgaa ccgggtcttt acttcgcgat gaacaccccg
    18781 cctgctggcc ttggcatcac catgcctaac ctgcatgaaa tgggtggcga gaacgcgccg
    18841 atcatcatgg cgcagttgaa agacgttacc gcacacgcgg cggcgaccgt cagctcctgg
    18901 ggcttcgtga tttcgccaga gcagatcctg caaaccgcga aagacattgg tgaaccttct
    18961 gtcctgaacc gtgcaggtgg tgcgccgacg ctggcggtag gtatcgcgca cgtgttccac
    19021 aaagtgctgc cgatggctga catgggcttc tggtatcact tcggtattct gttcgaagcc
    19081 ctgttcatcc tgaccgcgct ggatgcgggt acccgttctg gccgctttat gctgcaagac
    19141 ctgctgggta acttcatccc gttcctgaaa aaaaccgact ctctggttgc tggtatcatc
    19201 ggtactgcgg gctgtgtggg tctgtggggc tacctgctgt atcagggcgt ggtcgatccg
    19261 ctgggcggcg ttaagagcct gtggccgctg ttcggtattt ccaaccagat gctggcagcc
    19321 gtagcactgg tactgggcac cgttgtgctg attaagatga agcgcaccca atacatctgg
    19381 gtaactgttg ttccggctgt atggctgctt atctgcacca cctgggcgct gggcctgaaa
    19441 ctgttcagca ccaacccgca gatggaaggc ttcttctaca tggcaagcca gtacaaagag
    19501 aagattgcta acggtactga cctgacggca cagcagattg ccaacatgaa ccacatcgtt
    19561 gtgaacaact acactaacgc gggtctgagt attctgttcc tgattgtggt gtacagcatc
    19621 atcttctacg gtttcaaaac ctggcttgcg gtgcgtaaca gcgacaaacg tactgacaaa
    19681 gaaactccgt acgttccaat cccggaaggc ggcgtgaaga tctcttcgca ccactaaccg
    19741 tgtttagccc cgcttcggcg gggctttgtt cttatcagag tgaactatgt ttggtaactt
    19801 aggacaggca aaaaaatatc tcggccaggc ggcgaagatg ttgattggca ttccagacta
    19861 cgacaactac gtcgagcata tgaagaccaa ccatcccgac aagccgtaca tgagctatga
    19921 agaattcttc cgcgaacgcc agaacgcgcg ctacggcggc gatggtaaag gcggtatgcg
    19981 ctgttgttaa tggagagagc atgaacccga ttgcagttac cctactcacc ggttttttag
    20041 gcgcgggcaa aaccaccctg ctgcgccata ttcttaatga acagcacggc tacaaaattg
    20101 ccgtgattga aaacgaattc ggcgaagtct ccgttgatga tcaactgatt ggcgaccgcg
    20161 ccacgcagat caaaacgctg accaacggct gcatctgctg ttcgcgctcc aacgagctgg
    20221 aggacgcgct actcgacctg ctggacaatc tcgacaaggg caatattcag ttcgaccgtc
    20281 tggtcattga atgcaccggc atggccgatc ccggcccgat tattcagacc tttttctccc
    20341 atgagatttt atgccagcgt tacctgctgg acggcgtgat tgcgctggtg gatgcggtac
    20401 atgccgatga gcagatgaac cagttcacca tcgcccagtc ccaggtgggc tacgctgacc
    20461 gtattctgct gactaaaacc gacgtcgcag gcgaagtaga gaaactgcgt gaacgcctgg
    20521 cgcgcatcaa cgcccgcgca ccggtctaca ccgtcaccca cggtgacatc gacctgggcc
    20581 tgttgttcaa caccaacggt tttatgctgg aagaaaacgt cgtcagcacc aaaccgcgtt
    20641 tccactttat cgcggataaa caaaacgata tttcgtcgat tgtggtggaa ctggattacc
    20701 cggtagatat cagcgaagtt tcccgcgtga tggaaaacct gctgctggaa tcggcggata
    20761 aactactgcg ttacaaaggg atgctgtgga ttgacggcga acctagccgc ctgctgttcc
    20821 agggcgtcca acgcctctac agcgccgact gggaccgccc gtggggcgat gaaacaccgc
    20881 atagtacaat ggtgtttatc gggattcagc tgccggagga taagattcgg gcggcgtttg
    20941 cggggttgag gaagtaattt tagatgatgg ccgccatgtg gcggcctgaa ttatttttta
    21001 ttctgatcgc gcagcatcgc ttcacgtaaa attgcgttca ggcgagtttg atagccttta
    21061 cctggtcgct tcaaccattc catcacatca gcatcaatac gcaccgaagc ctgagttttc
    21121 aatggccgat aaaacttacc gcgttctgcg ttagaccatt gctcatcgct ggaaggtggg
    21181 atatcactgt aatcaatatc ttcgtcggat ttattcgcca gcgccttcag ttcggcctca
    21241 tgctgcacat cgagtgtaga ggaggtccct cgtttatgtt taaccatgct catagcgatt
    21301 ccgttcgttg tggatcatca aacactaaca ccgcatcttc aaagcggatg ccatgtttca
    21361 cccgattacc tttggcctta tttgcatccc attcaaactc cattggcata gttgccatcc
    21421 ctaattgtat atacaaaaat gtatgtacaa ttattctgca attatagcgt tgatgttgga
    21481 aatagcatca cgtttcgaat aatcaaccat gaaattgaca atgtgtgagt tggacttgat
    21541 tcatccaata gaatttctcg cctgacatgg cacactaaac acctcgctgt cgttaaggga
    21601 ttaccgtgaa aaactacaaa gtcattaccc cgctttttcc tacctatgcg caagttaagg
    21661 ctatgatgaa agccgtctca ggctattccc tgaaagcggt gcgcaatatg attactgcca
    21721 ttcacgagca aaccggaacg ccgcaaaaac ccgttgactg gtcggaacct gacctgtgga
    21781 tcagcgaacg cttaacgggt gaagatgccg acatcgcccg tcgtatttgg gataccgata
    21841 atcacatatt gaacccgcgc cacagctacg gctgctactt attcctcaac tatccccagt
    21901 ttgagctgat ggaaagcacg ccagacgata cctggcagcc cacctcgcgc gggcaaaaat
    21961 tcctgcaaga tgacgaaaaa acgctgcgtt cactggacga tcaggaaggc attctgcaac
    22021 ttcttgaact attagctggc cgcgaaatgt cgcgccgcgc cgatttattg ccggaatggc
    22081 aggccttttt gcatcagcat tcgaaattcg cgtctgcaag ttcagtgaaa agtacgctct
    22141 actcccgtct ctataacctt atcgaccgtg acatggttga ccgtgaaggc atgagctatc
    22201 gcatcaccga taccgggcgc gcctggcttg gaaaagcgtt gccggcacga aaaagcgatc
    22261 cacgtaaaga gctgctggaa gcggtgaaac gctacaacga gcagcaaaaa gagctgctgc
    22321 gcgaacaaat cagcacgatg aacccgtaca aatttgaaca actggttggt caattactgg
    22381 aagcaatggg ctacgagcag gtcgaagtga ccaaagcctc tggcgacaaa ggtgtggacg
    22441 tgattggcca ggtgcaggtc ggcatcacca ctattaccga agtggtgcag gtcaaaagaa
    22501 tgcagaacac tatcactcgc ccatatatcg accagttgcg cggggcgttg ccttaccaca
    22561 aagccattcg cggtacactc atcaccaccg ggaaatttgc ggcgaaatgt gcagaagcgg
    22621 cgctctttcc gggtgccgcg cccattacgc ttatcgacgg cgatcgttta ctggaactgt
    22681 taatcgaaaa taacgtcggc attcgccgca gcaatgcagt ggaattgctg gatgtggatt
    22741 tgcagttgtt tgatgagctg gagatggatt aaagggcgaa atccgccgcc tctaccatta
    22801 caatagcgac ccaatttatc tgaacagagt tcatcatggc caaagcacca actaaaaaag
    22861 ccaaagcaaa gaaaggcttt gaagaaacat tatgggatac agcaaaccag cttcgcggta
    22921 gcgttgagtc ctccgaatac aagcacgtgg tgttgagcct cgtgttcctg aaattcatca
    22981 gcgataagtt tgaagcacgc cgcaaaaaaa tgattgccga tgggcaagca gacttccttg
    23041 agatggaagt gttctaccag caggacaaca ttttctacct gccggaagaa gcgcgctggt
    23101 cttttatcaa acaaaatgcc aaacaggacg atattgcggt ccgtattgac accgccctct
    23161 cgaccataga gaaacgtaac ccaacgctga aaggcgcgct gccagacaac tacttcagcc
    23221 gtcagaatct ggaaaccaaa aaactggcgt cactgattga taccatcgac aacatcgaaa
    23281 cgctggcaca cgaaactgac gttgaaacgt tatcgaaaga agacctggtc ggacgcgttt
    23341 acgaatactt cctcggtaag tttgccgcaa ctgaaggcaa aggcggtggt gagttctaca
    23401 cgcccaaatg tgtggttacg ctgttaactg aaatgctcga acccttccag ggcaaaattt
    23461 atgacccgtg ctgcggctcg gcagggatgt tcgtgcagtc ggtgaagttt gtcgagagcc
    23521 atcagggtaa aagtcgtgat atcgcgctgt atggtcagga actgacagcc acgacgtata
    23581 aactggcaaa aatgaacctc gccattcgcg gtctttcagc taacctcggc gaacgaccgg
    23641 cagacacctt ctttagcgac cagcacccgg acctgaaagc cgactacatt ctggcgaacc
    23701 cgcccttcaa cctgaaagac tggcgtaatg aagccgaatt aaccaaagac ccacgttttg
    23761 ctgggtatcg tatgccgcca accggtaacg ccaactacgg ctggattttg catatgctct
    23821 ccaagctgtc ggctaacggc acagcaggtt ttgtgctggc aaacggttcg atgagttcta
    23881 acaccagcgg tgaaggcgag atccgtgcac agatgatcga aaatgatctg attgactgta
    23941 tgatcgccct acctggacag ttgttctaca ccacgcagat cccggtgtgt ttatggttta
    24001 tgaccaaatc gaaggctgcc gatccggcca aaggttatcg tgatcgtcag ggcgagacgc
    24061 tgtttattga tgcgcgtaac ctcggcacca tgattagccg cacaactaaa gagttaacgg
    24121 cggaagatat tgccaccatc gccgatacct accatgcatg gcgcagtacg ccggaagaac
    24181 tggctgcacg gattgcgcgt ggtgacagca agctggaaaa atatgaagac caggctggct
    24241 tctgcaaagt tgcgaccctg caagacatta aagataacga ctacgttctg acaccgggcc
    24301 gctatgtagg tgcagccgag caggaagagg acggcgtggc atttgagacg aaaatgcgcg
    24361 aattgtcgaa gacgttgttt gagcagatga agcaggcgga agaactggat cgtgcgattc
    24421 gccagaatct ggaggtgctg ggttatgggg agtaagtgga tagaagtatc actgggcgag
    24481 atttctgaaa aaattggaga tggaatccac ggaacgccaa cttataacaa ttcaggtaac
    24541 tattacttta ttaatgga
//
LOCUS       DAZGHF010000073        23883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGHF010000073 DAZGHF010000000
VERSION     DAZGHF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.073"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..368
                     /locus_tag="ACHK3A_002965"
     CDS             <1..368
                     /locus_tag="ACHK3A_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8741019.1"
                     /translation="KDILFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPI
                     NYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKN
                     CFAEQEILSEFPQPVSLAG"
     gene            complement(411..1562)
                     /gene="ydiO"
                     /locus_tag="ACHK3A_002966"
     CDS             complement(411..1562)
                     /gene="ydiO"
                     /locus_tag="ACHK3A_002966"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO8741020.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1576..3171)
                     /gene="ydiF"
                     /locus_tag="ACHK3A_002967"
     CDS             complement(1576..3171)
                     /gene="ydiF"
                     /locus_tag="ACHK3A_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO8741021.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3317..4075)
                     /gene="aroD"
                     /locus_tag="ACHK3A_002968"
     CDS             complement(3317..4075)
                     /gene="aroD"
                     /locus_tag="ACHK3A_002968"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO8741022.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDIIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4106..4972)
                     /gene="ydiB"
                     /locus_tag="ACHK3A_002969"
     CDS             complement(4106..4972)
                     /gene="ydiB"
                     /locus_tag="ACHK3A_002969"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO8741023.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4984..6249)
                     /gene="ydiN"
                     /locus_tag="ACHK3A_002970"
     CDS             complement(4984..6249)
                     /gene="ydiN"
                     /locus_tag="ACHK3A_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8741024.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFATFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(6476..7690)
                     /gene="ydiM"
                     /locus_tag="ACHK3A_002971"
     CDS             complement(6476..7690)
                     /gene="ydiM"
                     /locus_tag="ACHK3A_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8741025.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALLLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(7790..8146)
                     /gene="ydiL"
                     /locus_tag="ACHK3A_002972"
     CDS             complement(7790..8146)
                     /gene="ydiL"
                     /locus_tag="ACHK3A_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO8741026.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTDDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTVFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8282..8389)
                     /gene="rprA"
                     /locus_tag="ACHK3A_002973"
     ncRNA           complement(8282..8389)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHK3A_002973"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8575..9687)
                     /gene="ydiK"
                     /locus_tag="ACHK3A_002974"
     CDS             complement(8575..9687)
                     /gene="ydiK"
                     /locus_tag="ACHK3A_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO8741027.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            10074..13130
                     /gene="ydiJ"
                     /locus_tag="ACHK3A_002975"
     CDS             10074..13130
                     /gene="ydiJ"
                     /locus_tag="ACHK3A_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO8741028.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSSMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWVAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13127..13537
                     /gene="menI"
                     /locus_tag="ACHK3A_002976"
     CDS             13127..13537
                     /gene="menI"
                     /locus_tag="ACHK3A_002976"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO8741029.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13637..13825
                     /gene="ydiH"
                     /locus_tag="ACHK3A_002977"
     CDS             13637..13825
                     /gene="ydiH"
                     /locus_tag="ACHK3A_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO8741030.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14374..14742
                     /gene="sufA"
                     /locus_tag="ACHK3A_002978"
     CDS             14374..14742
                     /gene="sufA"
                     /locus_tag="ACHK3A_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO8741031.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14751..16238
                     /gene="sufB"
                     /locus_tag="ACHK3A_002979"
     CDS             14751..16238
                     /gene="sufB"
                     /locus_tag="ACHK3A_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO8741032.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16248..16994
                     /gene="sufC"
                     /locus_tag="ACHK3A_002980"
     CDS             16248..16994
                     /gene="sufC"
                     /locus_tag="ACHK3A_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO8741033.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLL
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16969..18240
                     /gene="sufD"
                     /locus_tag="ACHK3A_002981"
     CDS             16969..18240
                     /gene="sufD"
                     /locus_tag="ACHK3A_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO8741034.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18237..19457
                     /gene="sufS"
                     /locus_tag="ACHK3A_002982"
     CDS             18237..19457
                     /gene="sufS"
                     /locus_tag="ACHK3A_002982"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO8741035.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19470..19886
                     /gene="sufE"
                     /locus_tag="ACHK3A_002983"
     CDS             19470..19886
                     /gene="sufE"
                     /locus_tag="ACHK3A_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO8741036.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            20035..21039
                     /gene="ldtE"
                     /locus_tag="ACHK3A_002984"
     CDS             20035..21039
                     /gene="ldtE"
                     /locus_tag="ACHK3A_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO8741037.1"
                     /translation="MKCASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYNYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21103..21339)
                     /gene="lpp"
                     /locus_tag="ACHK3A_002985"
     CDS             complement(21103..21339)
                     /gene="lpp"
                     /locus_tag="ACHK3A_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO8741038.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21650..23062)
                     /gene="pykF"
                     /locus_tag="ACHK3A_002986"
     CDS             complement(21650..23062)
                     /gene="pykF"
                     /locus_tag="ACHK3A_002986"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO8741039.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(23070..23264)
                     /locus_tag="ACHK3A_002987"
     CDS             complement(23070..23264)
                     /locus_tag="ACHK3A_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741040.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            23619..23828
                     /gene="fumD"
                     /locus_tag="ACHK3A_002988"
     CDS             23619..23828
                     /gene="fumD"
                     /locus_tag="ACHK3A_002988"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO8741041.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         6216 a         5724 c         6013 g         5930 t
ORIGIN      
        1 taaaagatat tcttttctat ctgaataata attatcgcga aaaaatcact ttagagcagt
       61 tatcgaaaaa gtttcgtgcc agcgtcagtt atatttgcca tgaatttacc aaagagtatc
      121 gcatttcccc tattaactat gttattcaac ggcgtatgac ggaagcgaaa tggtcactca
      181 ctaatactga attatcacag gcagagatct cctggcgtgt gggttatgaa aatgtcgatc
      241 actttgccaa actgtttttg cgccatgtcg gctgttcgcc cagcgattac cgcaggcaat
      301 ttaaaaactg ttttgcggaa caagaaatcc tatctgaatt tcctcaacca gtaagtcttg
      361 ccggataaat ctggcggcgc agtttgttaa gaagctgcgc cgcctgcaga ttatttgttc
      421 tgataatctt tcaggatctg ccgacctgct acgtaaatca taatttcgtc tgtaccgccg
      481 ccgatacgtt cacaacggac atcacgccag aagcgggaga cgcgcgcctc atcggtatag
      541 cccagaccgc ccatgatttg aatcgcatca tcaatgactt ccattgcggt acgtgcgcag
      601 tacagttttg ccagcgccgc gctggtgcgc agtgactgat gctgatcggc ttgccatgcc
      661 actttcaaca ccatgttgcg catgttgtcg atcttaatcg ccatcagcgc cagtttttcc
      721 tggatcatct ggttatgacc aatgggctta ccaaaagcga tacgctggtt ggcatagcgg
      781 gcggcatctt cgaatgcgca ttcggcaaag ccggtgctgc gcgcggcgtt gatcaggcgc
      841 tccatctcaa agttgtacat cacattgagg aaacccattc cttcttcgcc caccatgtcg
      901 ctctcttcaa cttccacgtt gtcgagatag acttcgcagg tgctgagcat atgccagccg
      961 attttatgca gtggattgat tttaatgccg ggcttactgg agtcgaccca ccacagagta
     1021 aaggcctttt tcggatcttt cggttgcgga tcgcgcgcca acaccagcat atatggatac
     1081 tctttcgcac cggtaataaa ggttttctgt ccgttgatgt aaaccttgcc gtttttacgc
     1141 gtgtaagtgg tagtggcact gttgttgtct gagcctgcgc ctggctctgt caatgccagg
     1201 gcataggcgg gatcacccgt ttccagggtg ctttccgccg ttttacgtag ctgctctgca
     1261 gaaccgaaac ggcgcatact gtgaatacat tgaccgttgg tgatcaaaaa tgccggggca
     1321 ccgcattttg acacttccat cagcgccagc atttgggtga cgtaatccgc agggatacca
     1381 ccaaattctt ccggcacgcc aagcatggaa ataccgttat ccgccagtgc ccgcataaat
     1441 tcacgcgggt atgtcccgtt ttgatcgcag gtgcggaaat actcttccgg aaagttagtc
     1501 gtaatcagtt cgcgaatact ggccagcagc agttcttgtt cttcagttaa agaaaaatcc
     1561 atcattatac tcctgttaat gagccgcttc aggcaggaca aaacccatcg ccgcatcgat
     1621 aaataatctt tcgtccatca gtttgagttc tggcgaaatc actggggtga aatccatttt
     1681 gtcgagaata tctttttgta aatcgacgcc aggagcgatt tcgattaaat gcaggccgtc
     1741 ttctttcagc gtgaatacgg cgcgctcagt gatataacga acatccagcc ctcgctcgag
     1801 cgcgattttt ccgctaaaag taatttctgg tagttcccga ataaatttct tcacccgtcc
     1861 ttcctggacg atatttaatt tgccgtcggt aatttctgtt tttaaactgc ccgcagttaa
     1921 tgtgccgcag aaaacgattt tcttcgaagt ggcactgata tcaataaatc caccggtgcc
     1981 catgatttta ccattgaatt tatgcacgcc gacgttaccg tgctggtcga cttcagcaaa
     2041 actcaaataa caaacatcca gaccaccacc gtgataaaaa tcaaactggg acgtcatatc
     2101 cagaatggcg cgggtattca cgttcgcgcc aaaggcgatc ccctgtgaag taataccgcc
     2161 aatcggacct gtttctaccg tcagaataaa gtcatcagca caaccttctt cccgggcgac
     2221 caggccaatg ccgtcagcaa taccgacgcc gacattcccc actgcgcctt tgcgcatttc
     2281 gaataatgca cgccgtgcaa ctaatttacg ttggtttagg ggcaggctaa gtttggtgct
     2341 gtcatcaagg gtgaagtcac cagaaataaa gcggttaacc ggcgctccgc catacagttg
     2401 ggtttgatcc ggatcgacca ccacaatatc caccagataa cccggaatac ggacagattt
     2461 aggatgcagc gtggctttct taaccatttt ctgcacctgc atcatcacaa taccgccgtt
     2521 attgtgcacc gcctgagcaa taaccaatgc gtcgagatac atcacctcat cttcaaaagt
     2581 ggcgtagcct tcgctgtcgc aggtggtagc gcgaatgaag gcgatatctg gcgctatcgc
     2641 tttgtaatag agatattctt tgttatcaaa ctcgaccagt ttaatcaggt cttctttagt
     2701 gacttcattc agtttgccgc cttgctggcg tggatcgaca aatgttccga tgccaatatc
     2761 actaataata ccaggctggt gggctgcggc ggcgcgtaag gtttgtgtaa gtacaccttg
     2821 tggatagtta taagcaataa ttttattttg ttctgcgaga tcagaaatac gcggcgattg
     2881 tccccagtga ccgcataatg cccatttcac caggccttct tgcgccagcg gactaatacc
     2941 acggtcggcg cgatcgccaa gccctgttgg actaataatc gataaattac gtggtgtttg
     3001 agtctgttta tatttatcag caagagcagt aattaacgtg gtggcttcca gaataccgcc
     3061 gccagcgcct aacacacaaa gtgttgcttc gtcgggaata taattcaccg cttcctgtgc
     3121 cgacaggacc ggcactcgtc cattaatgcg aggtggtttt acaggtttca ttgttaattc
     3181 ctgattcagg tgtgattttc cctacgccag atggagaatt agcgcacaga gactcacgaa
     3241 atatttttat taaatacggt taaaaatact ttttttagtt cggcggggga gagtattccc
     3301 gccgaaatat tattgcttat gcctgatgta aaatagttaa taccgtacgc aaatcattta
     3361 ccgagatttg ccctggcgca gaggcttttt ttaccgcacc aaaagttgcc gccgagccaa
     3421 atacttcacc agccagacga gaaattacgc cagtttttgc catcgacatc gtgatgattg
     3481 gacgatcggc atactgctcc tgcatctcca gggtcgcagc aagcaacgtc agcacatcgc
     3541 tggtactttg cggcatcagc gcaatcttag gaatatcggc atcgaaggac tgcattttgc
     3601 gcagacgggc aatgatttct tcggcttccg gcgttttatg gaagtcatgg ttggacatga
     3661 caactttcac atcatgcgcg tgggcgtagg cgacggtttc tttaacctga tcatcgccgg
     3721 taaataactc cagatcgatt atatcaacca ggccgctgtc gatggctgca cgattgagtg
     3781 caatataagc ctcggtggaa atcgcctgct cgccgccttc tttggcactg cggaaggtaa
     3841 acagcagcgg tttttctggc atggtttcac ggagaatttt tgctgccgct atgacagact
     3901 ccacattgga gagatcggca tagtggtcca cacgccattc cagaatatca aagtccgctt
     3961 cacgataggc gagagcttcg gatttcacgc tggcgatatc tttcgccatc agcgagacga
     4021 tgattttagg tgcgcccgta ccaatgacga gatcttttac agttacggtt ttcatttttt
     4081 accctttctg cacgcggtca gcctgtcagg caccgaaccc catgacctgt ttaacatatt
     4141 ccagagggaa atctttgcca gtccataacg tgaactgttc agccccttgc cacaacaaca
     4201 tgccgtatcc atcaatcgtt ttgcaacccg cttgttgcgc ctgctgcaat aacttcgtca
     4261 tatgagggtt atacacgcat tcagtgacca gaagtcccgg atgtaacaga ctgatatcat
     4321 taaccaatga ttcattctca aggggtttca tacccacttt ggtgccattg gttaaaatgt
     4381 cggcggaagc cagggcttct gcaaaggctt gctgatcggc gagatcggtg actgtcacga
     4441 cgcaatcggt gttttcgtta acccgctgcg cgaaggcgag agctttatcg aagaactcat
     4501 cccgacggtt aaagagttta atttctttta aaccttcaat tgccccctgc gcgccaattg
     4561 ccgttgaggc accaccggcc cctaatagca ccatcgtttt gcctttgata tcaaaaccgc
     4621 tctctttaat ggcgcgaata tgacccgtgc cgtcggtgtt atagccacgc agatagccat
     4681 catcattaac gatggtgttg atggctccca ccagtttggc cgctggcgtt aattcatcaa
     4741 catattcaca cgccagttgt ttgttcggca ttgatacacc agttccgcgc attttgaggg
     4801 cttttaatcc ttcaattgct ccgggaaaac tatcgttatc cacttcaaag gccatatagg
     4861 taaatggcaa tcccgctttt tctaaggctt tattctgcat ttcgggcgat aaactgtggc
     4921 ggatgggata ggccatcaac ccaatcaatt cgtattttgc ggtaacatcc ataattaact
     4981 cctttaacct ctatgcttaa tcgtgtttaa ccgggtgcta aatttatgct cgccaaaccg
     5041 cacatcattt tcaggaataa tgaaaacgcg gtaatagcgg ataaaaacaa taattgcggt
     5101 aatcagggcc agcagcgcga aagtaaaatc gagaacaatg atatattgca ggccgatgtt
     5161 cgacagataa ccggtaatca gcggaataac aaagttagcc agtccaccca tcatcatata
     5221 tatactggtg actttggctt tgctttttgg gaaaaactct gacatgaccg aaacgccgag
     5281 ctgtaaaatg ccgccagctg ctgagaaacc gataacaaag gctccggcat tacacaccaa
     5341 tggagaaggg tacaggtaga taatggcagc tgttattgtt gccagtgcag agttaaatac
     5401 attagcccag atgggccgga ccattttttt cagcagggcg gcaaaaataa agacacagac
     5461 cagcgaaccc atactgtaat aagagatggt ttttaatgcc tcagcttctg acataccagc
     5521 aaaagccatc gcatatttgg gcatccacac cacaatcaca taaaaggtcg cgaatgcggc
     5581 tacaccgaac agtactgatg aaacaccttc cagccagact aacggtttgc tgttcatttg
     5641 cggtaattca ttagctacgc tggcgtccac caactggctg gggaatttgc ttttcaacag
     5701 catcagcgtg atcagtacaa acagaatacc cggaataatc agcccatagc cgtaccagat
     5761 gttattgagc aacatatagc tcaccagcat tgggtagaac atttgcccga atgacaccat
     5821 cgctttaacc agtatgaccg ccgaaccaga ggctttcgga aagcattcca taagcgcggg
     5881 gtagccaccc gtatccagcg ctgagttagc gatacctacg cacactgcca gacagtaggc
     5941 gagagttaaa ttcgggcaag caggaatacc aaagaagaat agcagataca ttattactgc
     6001 cattaatatc accgcccgac gaccaaactt atcggagatc acaccgaaga ataaaatact
     6061 gatcaatcgc cccaatccga taccggaaat taaataggca atgcccgcgt tgtcagtgga
     6121 aaacttttct gccaaagatg tcatgttttg ggcaagcgta ataacactaa tgccgtgcag
     6181 gaaatagctg aagtaaatac aaagaacagc caggataaat ggcgtgctga aagccttatt
     6241 ttgagacatt ttcacagctc cttgctgaaa gataatttcg ttaccgtacg tattgttctc
     6301 cgacggttta cataattact atactgaaga taattttccc tgggtacatg gcataaagtt
     6361 tatgtttcca ggaatattgc atgtcatagt ttgtttttga tcgcgatttt ccttagttag
     6421 cgcagaaaga gttatttatc cgtaagatct tgcatgacct gaaaagaata ttgcattacc
     6481 cacccggagc gacattttcc tttagcgagt gatgccgcgt ttctttgcga ctgcgtaagc
     6541 caataaacag tgccagtata aagccgatgg cagcgatggc ggtatcgaac cacataatat
     6601 cggcaatact tctttgcgaa agatgagccg taatcaacgg aatagtaaag gttgcaatgc
     6661 tgcccgcact gtaatagatc cccgttgctt ttcctttagc gtagggaaaa cgttccgcca
     6721 ttaacgtcag gccaatttgt acaacaccgc ctgcggaggt aaaaccgatg acgaaagcaa
     6781 atattatcac cacataaaat gtgggatgca ggcagacggt gagcagggcg ataaatgaga
     6841 taaaggtgta cagcatcagt aatgttgttg ggcgaacggt attacgaatg agtggagcgg
     6901 taataaatac acaaagcagc gaacccacgg tgtagatact gagtaatttg atcgacatgg
     6961 tgtatgacat gccggcgaca aattgtccgt attgtgccag ccactggcta accagataaa
     7021 acgttgccat tgagatatag ccatataggg aataactggc taaatcgata attgaacagc
     7081 gatgttccgt agagctggtg gttttcttta tgacaggtaa gtgacgaccc ggatggggtg
     7141 ggaacgtaca acggtataaa agcagagcgt taataaacat aatgcctgcg gcaatcataa
     7201 aggaccaacc gaaccacagt tcagcccaca ctaacaggct gataattagc ggtaataaaa
     7261 attgtccgct ggaaacaaat gctttaatta aaatattggc tgtacccggt gagcgtggaa
     7321 aagcttccat caaactggga taggtgcctg catcgagaaa actgtttgcc attcccgcca
     7381 gaaagccaaa aacataagcg ataatgatgt tattggtgtg caggatgcca aaaaagaagg
     7441 ccatatagca gcacatcccg agcatgataa aagggcggcg accaaagcga tcggataata
     7501 atcctgcaaa aagcaagaca cttaatcgac caatgcccag tgatgagata actatcgaga
     7561 cgcccgcggc attagtctgc caaagcgtct ccagcgaggc catattcagg ctcatcaaaa
     7621 tgacgcccat accatgcacc aggtaattaa aatacaaccc aagtgcggta gggtaatagg
     7681 gatttttcat aggttctact tagcaaaggt gctttaaaaa gtgtagagat gttgtgtcag
     7741 tatccgttca ttattgttgt aacaaagaca attcctttgt gtggagaaat taacaaagcc
     7801 gctccagatc gtgtgcatat aattcagcgg ctaccgattg atagatcttc cagtcaataa
     7861 aattgccatc agggtagact tgttgaaaca cggttaagtc ggggaaaaaa cgcatggtgt
     7921 tgttgccgat gcggttattt attttctcta tgatggcatg aagatgtttt tttcgttgct
     7981 gacgcatagc caacagttgc tcgactacac gatcaggaat ggcgcatttg ccattttccc
     8041 actgacgcca gctttcactg tcatcggtct gggcaatcca ggtcgcgcat tcatcaatag
     8101 tcatggcaaa aatatggcga agtgcctgga gttcataagc gttcatcgtg taatggctgc
     8161 atgaaataaa caataaaata taaataacat tatcagcctg ctgacggctt gaagagagtc
     8221 acagtatctt gtgcaacatt attggaaagc gtgaggggcg agatagcaaa gcggaaaaat
     8281 gttaaaaaaa agcccatcgt gggagatggg caaagactac acacagcaat tcgttgtttc
     8341 actcagggga tttccatgct tataaatcaa tatgttgatt tataaccgtg agctaatagt
     8401 aggcatacgg actttttgcg tcgatcagat tcgtctcatt agtttaaaaa ctgtaaagaa
     8461 tttgcgacag gtactaccag ctgaatggcg gtaagcagga aaatatgggg aagagcaagc
     8521 aaatatcaat tagcaggggt taacaatcag gcggcagagc cgcctgatga gaaattactt
     8581 attcgatttc tcgatttcgc caagttcttc gagaatttct tccggttggt ccgtcggcgg
     8641 tgggacttct tccacccacg cggcaaaaag acgccaggaa acggctaaca gaaccggacc
     8701 aataaacagg ccgatcatcc cgaaagcaat caaaccacca ataacaccag agaggatcag
     8761 gatcagcggt aaatcggcac ccatgcgaat taacattggg cggatgacgt tatccagcgt
     8821 gccaaccaca ccgctccaca ctaacaatac cgttccccag gtggtatcac cggtccagta
     8881 gagccagata atcgccggaa tcagtaccgg caacggtcca agctggacaa ggcaggagag
     8941 gatcattaac accgtgagca aagttgcata aggtacgccg gatacggcga ggccgatacc
     9001 gccaagcact gcctgtacta acgccgtcac caccacaccc agcgccaccg cgcggatagc
     9061 ctgtgccgcc agtagcacag cagcatcacc gcgaacgcct gccagacggg ttgcaaaatg
     9121 gcgaatgcct tgtgccacct gttcaccgcg ccagtacagc agggcactga agagaagcat
     9181 cagcgcacaa tgcaccataa agcgcccgat atgcgccgcc tgcccaacga accaggtggt
     9241 ggtggtgcca atataaggac ggactttcgc catgatcgcc gtacccccca tatccagcaa
     9301 gttgtgccag cctgcatata gcttcgcgcc aatcaccgga atggtattaa gccacgccag
     9361 atcgggtaac gtcatgtcac cgctggaaat ggctttaatt agcggaccgc tgccatcgac
     9421 aatactgtta accaacaagg caatagggat gataaacacc atcactaata acagcgtcat
     9481 caccagaacg gcgagagagc ggcggccaaa catgatcttt tgcaaacgta acaataccgg
     9541 ccaggtggcg ataaccactg taccggccca tgcaaagccg agaataaagg gttgaacaat
     9601 ccacagacat gccacaatca tgatggctaa aaacagcacc gaaagcagaa tttgtgcgac
     9661 atccctgggc tgacgaacat ttaccataac tacttttcac cttttttgtg cgtcaaatcg
     9721 tcgacgcgac gtgaacacgc gaaactcacc tgcataatga tgagcaattt cagcggtttt
     9781 taacaggtcg attctgccct taattctgat gggcgtgtaa gaaaaaaatg tggtacaaca
     9841 actcaggcaa cacgcaaacg atttactcgt cgtatttcaa attgcatgtg ctgcggctac
     9901 gttcgttcac cccagtcact tacttttgta agctcctgga gttcactcgc ttgccgcctt
     9961 cctgcaactc gaaatccata gagtaaacaa acatcacctt tcagtgcgct attagcagcc
    10021 tgaaagaaca gtgtatcgat ctacatcaca gaccgcagga aagggtcaat ataatgattc
    10081 cacagatttc ccaggcaccc ggcgtcgttc aattggtgct taattttttg caagagctgg
    10141 agcaacaggg ttttactggc gatacggcga caagttatgc cgatcgtctg acaatgtcga
    10201 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
    10261 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
    10321 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
    10381 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
    10441 aggccggggt gataaaagat caactcaatc agtacctgaa accgttcggc tacttttttg
    10501 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
    10561 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
    10621 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgaactg gcggaaacgc
    10681 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
    10741 agcaacgcca gttaatcatc gacaacttcc ccaagcttaa ccgctttctt accggttacg
    10801 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
    10861 gttcagaagg gacgctggcc tttattaccg aagcgcgact ggatattacg cgcttgccta
    10921 aagtgcgccg tctggtgaac gtcaaatatg actcttttga ctccgcactg cgtaacgcgc
    10981 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
    11041 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat gtgcctgaca
    11101 aagaaatgct cgggctgaac attgtggaat ttgctggtga tgatgagacg ctgattgatg
    11161 agcgggtaaa tgcactctgt gcgcggttgg atgaactgat tgccagccaa caggcaggtg
    11221 tgattggctg gcaggtgtgc cgcgagctgg cgggtgttga acgtatctat gcgatgcgca
    11281 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
    11341 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
    11401 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
    11461 tccgtcctgc gctggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
    11521 atgacgtagt ggcgcttacc gcgaaatacg gtggtttgtt gtggggcgaa cacggcaaag
    11581 gttttcgtgc cgaatacagc cctgcgtttt tcggtgagga actttttgca gaactgcgca
    11641 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
    11701 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
    11761 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
    11821 tatgcttcaa ctttgatgcc cgtagttcga tgtgtccgtc gatgaagatc acccagaacc
    11881 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
    11941 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
    12001 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tacgacttct
    12061 cacacgaagt caaagaggcg atgtcgggct gtcttgcctg taaagcgtgt tcgacccagt
    12121 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
    12181 gttatttacg cccgcttcgt gaccacctcg ttgctacggt cgagagctac gcaccgctga
    12241 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaagctct
    12301 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc agtcccctcg ctacaacaac
    12361 aaatggtggg gcatcgctcg gcgaatatga cgctggaaca gcttgaagct ctcaatgcag
    12421 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
    12481 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
    12541 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
    12601 cggcgaaaaa gacggcggat ttcctcaacc gtatggcgaa gctggggatg ccaatggtgg
    12661 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
    12721 aacgtggcga gtttaacgtc ttactggcga atgaatggct ggcaagcgca cttgactcac
    12781 agccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaagtta
    12841 ccgctttgcc gggcgcgcca gcacaatggg ttgcgatatt tgcccgtttt ggcgcgaaac
    12901 tggaaaatgt cagcgtgggg tgctgcggca tggcagggac ttacggacat gaagcgaaaa
    12961 accataaaaa ttcgctcggg atctatgagt tatcctggca tcaggcgatg cagcgactgc
    13021 cgcgtaatcg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggtcgaag
    13081 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
    13141 aaaaatcacc ctggaagcac tgaatgccat gggtgaagga aacatggtgg ggttgctgga
    13201 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
    13261 gacaaagcag cctttcgggt tgctgcatgg aggggcatcc gtggtactgg ccgaaagtat
    13321 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaggtggttg gtctggaaat
    13381 caatgctaac cacgtccgct cggcacgaga agggcgggtg cgcggcgtat gcaaaccgtt
    13441 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    13501 gtgctgttcg tcacgattaa cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    13561 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    13621 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    13681 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    13741 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    13801 tgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaattttgc caaatcgacc
    13861 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta tccgcctgat
    13921 attgttcaaa aataatacta atcgcagttt gtgttaaaac ggcgggttag ctttatgaga
    13981 ttattcttat cgccccttca agagctaagc cactgtgagt gccggagata agcgccggat
    14041 ggggtagaaa cccttaagcc tgtgtcgcac agacttaagg gttttcttat ttctgaacgc
    14101 tgcacttttt tacctatccc ttaaatagtc attttcaata caactttttc tttttcatca
    14161 cagccttaat gctccttaga taataatcat tatctaacaa tgagatacct aattcttagc
    14221 gtgcctgtta acccacacat cagggtctat gcttattaaa agcaggtaaa aagcatgttt
    14281 tacgctaaag tccctgcgtt tgctgggttg aactgataat cattatcact aacatgctgt
    14341 tatacgctga aagcgatgaa gtgaggtaaa tcgatggaca tgcattcagg aacctttaat
    14401 ccacaggatt tcgcctggca gggcttaacg ctgacacccg cagccgctgt ccacatccgc
    14461 gagctggtgg caaagcagcc gggtatggtc ggcgtgcgct taggcgtgaa gcaaacgggc
    14521 tgcgcgggct ttggctatgt gctcgacagt gttagcgagc cggacaaaga cgatctgctg
    14581 tttgaacacg acggcgcgaa gctgtttgtc ccgctgcaag cgatgccgtt tattgatggc
    14641 acggaagtcg atttcgttcg tgaaggactt aatcagatat tcaaatttca caaccctaaa
    14701 gcccagaatg aatgtggctg tggcgaaagc tttggggtat aggcggtact atgtctcgta
    14761 atactgaagc aactgacgat gtcaaaacct ggaccggcgg cccgctgaat tataaagaag
    14821 gattcttcac ccagttagcc accgatgagc tggcaaaggg gataaacgaa gaggtggtgc
    14881 gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct ggagtttcgt ctaaatgcct
    14941 atcgcgcatg gctggagatg gaagaaccgc actggttgaa agcgcactac gacaagctga
    15001 attatcagga ttacagctac tattcggcac catcgtgcgg taattgtgac gacacttgtg
    15061 cgtctgaacc cggcgcggtg cagcaaactg gcgcgaacgc ctttttaagt aaagaggtgg
    15121 aggcggcgtt tgagcagttg ggcgttcccg tgcgggaagg caaagaggtg gcagtggatg
    15181 ccattttcga ctctgtttcg gttgccacta cttatcgcga aaaactggcg gagcagggaa
    15241 ttattttttg ttcctttggc gaggcgatcc acgatcaccc ggaactggtg cgtaaatatc
    15301 tcggcaccgt ggtgccgggg aatgacaact tctttgccgc acttaatgcg gcggtagcct
    15361 ctgacggtac gtttatttat gtgcctaaag gcgtgcgttg cccgatggaa ctttccacct
    15421 attttcgcat taacgcggaa aaaaccgggc agtttgagcg caccattctg gtggccgacg
    15481 aagacagcta cgttagctac attgaaggct gttccgctcc ggtgcgtgac agctatcagt
    15541 tacacgcggc ggtggtggaa gtcatcatcc ataaaaacgc cgaggtgaaa tattccacgg
    15601 tacaaaactg gttccctggc gataacaaca ccggcggtat tctcaacttc gtcaccaagc
    15661 gtgcgttgtg cgaaggcgaa aacagcaaaa tgtcatggac gcaatcagaa accgggtcag
    15721 cgattacgtg gaaatatccc agctgcattt tgcgcggcga taactccatt ggtgagtttt
    15781 actcagtggc gctgaccagc ggtcatcagc aagcggatac cggcaccaag atgatccaca
    15841 tcggtaaaaa caccaaatcg accattatct cgaaagggat ctctgccgga catagtcaga
    15901 acagttatcg cggcttagtg aaaatcatgc cgacggcaac caatgcgcgc aatttcactc
    15961 agtgcgattc aatgctgatt ggcgctaatt gtggggcgca taccttcccg tatgtcgaat
    16021 gtcgcaataa cagcgctcag ctggagcacg aggcaacgac atcacgtatt ggtgaagatc
    16081 aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga tgccatctcg atgattgtta
    16141 acggtttttg caaagacgtg ttctcggaac tgccgctgga atttgccgtt gaagcacaaa
    16201 aactcctcgc catcagtctt gaacacagcg tcggataagg aataaacatg ttaagtatta
    16261 aagatttaca cgtcagcgtg gaagataaag ctatcctgcg tggattaagc ctcgacgttc
    16321 gtcccggcga agttcacgcc attatggggc caaacggttc tggcaaaagt accttattgg
    16381 caacgcttgc cgggcgagaa gattatgagg tgacgggcgg cacggttgag ttcaaaggca
    16441 aagatttgct tgcgctgtca ccggaagatc gtgcaggtga aggcatcttt atggccttcc
    16501 agtatccggt ggaaattccc ggcgtcagta accagttttt cctgcaaacg gcacttaatg
    16561 cggtgcgcag ctatcgcggc caggaaacgc tcgaccgctt tgattttcag gatttgatgg
    16621 aagagaaaat cgctctcctg aagatgccgg aagatttatt aacccgttcg gtaaacgttg
    16681 gtttttccgg cggtgagaaa aagcgcaacg atattttgca aatggcggtg ctggaaccgg
    16741 agttatgcat tcttgatgag tcagactccg ggctggatat tgacgcatta aaagtggttg
    16801 ccgatggcgt gaactcgctg cgtgacggca agcgttcatt catcattgtt acgcactacc
    16861 aacgcattct cgactacatc aagcctgatt acgtccatgt gctgtatcag ggacgaattg
    16921 tgaaatccgg cgatttcacg ttggtcaaac aactggagga gcagggttat ggctggctta
    16981 ccgaacagca gtaacgcact gcaacagtgg catcatttgt ttgaagctga aggggcgaaa
    17041 cgctccccgc aagcacagca gcatttacaa caattgctgc gtaccggact gccgacacgt
    17101 aaacatgaaa actggaaata tacgccgctg gacgggctga ccaatagcca gtttgtcagc
    17161 attgcggggg agatatcccc acagcagcgt gatgccttag cgttaacatt agacgccgtg
    17221 cggctggtgt ttgtcgatgg acgttacgtg cccgcactga gcgatgcaac tgaaggcagc
    17281 ggatatgaag tgagcattaa cgacgaccgt caggggttac ccgatgctat tcaggcggaa
    17341 gtgtttctgc atttgacgga aagcctggca caaagcgtga cgcatatcgc cgtgaagcgc
    17401 ggtcaacggc cggcaaagcc attgctgtta atgcatatca cccagggcgt ggcaggtgaa
    17461 gaggtgaata ctgcccatta ccggcatcat ctggatctgg cggaaggtgc cgaagcaacg
    17521 gtgatcgaac attttgtcag ccttaatgat gctcgtcact tcaccggcgc acggttcact
    17581 atcaacgtcg cagcgaacgc ccatttgcag catatcaagc tggcgtttga aaacccgctc
    17641 agtcaccact ttgctcataa cgatttgttg ctggctgacg atgccaccgc atttagccac
    17701 agtttcctgc tgggtggcgc agtattacga cacaacacca gtacgcaact caatggcgaa
    17761 aacagcacgc tgcggatcaa tagcctggcg atgccggtga aaaacgaggt gtgtgatact
    17821 cgtacctggt tggaacacaa taaaggtttt tgtaacagcc gacagttgca caaaactatc
    17881 gtcagcgaca aaggccgcgc ggtatttaac ggtttgatca acgtcgcgca gcacgccatc
    17941 aaaacggatg gtcagatgac caacaataat ctgctgatgg gcaaactggc ggaagtggat
    18001 acgaaaccgc agctggaaat ctatgcagat gatgtgaaat gcagccacgg cgcgacggtg
    18061 gggcgtattg atgacgaaca gatgttttat ctgcgctcgc gcgggatcaa tcagcaagat
    18121 gcccagcaga tgatcattta cgcctttgct gccgaactga cggaagcact gcgtgatgag
    18181 gggcttaaac agcaggtgct ggcccgaatc ggtcaacgac tgccaggagg tgcaagatga
    18241 ctttttccgt cgacaaagtg cgggccgact ttccggtgct ttctcgtgag gtaaacggtt
    18301 tgccgctggc ttatctcgac agcgccgcca gtgcgcagaa accgagccag gtgattgatg
    18361 ccgaggctga gttttatcgt catggctacg cggcggtgca tcgcggtatt cataccttaa
    18421 gcgcccaggc gaccgagaaa atggagaacg tgcgcaagcg ggcatcgctg tttattaatg
    18481 cccgttcggc ggaagagctg gtgttcgtcc gcggcacgac ggaagggatc aatctggtcg
    18541 ccaatagctg gggcaacagc aatgtgcgag cgggcgataa catcatcatc agtcagatgg
    18601 agcaccacgc taacattgtc ccctggcaga tgctttgtgc acgcgttggc gcagagctgc
    18661 gtgtgatccc gctcaacccc gacggtactc tgcaactgga gacgctgcct acgctgtttg
    18721 atgagaaaac tcgtctgctg gcaattactc atgtctccaa cgtgcttggg acagaaaatc
    18781 cgctggcgga gatgatcaca cttgcgcatc aacatggcgc aaaagtgctg gtggatggcg
    18841 ctcaggcggt gatgcatcat ccggtggatg ttcaggcgct ggattgcgat ttttacgtgt
    18901 tctccgggca taaactgtat ggccctaccg gaattggcat tctttatgtc aaagaagcct
    18961 tgttgcagga gatgccgccg tgggaagggg gcggttctat gatcgccacc gtcagcctga
    19021 gtgaaggcac tacctggacc aaagcaccat ggcggtttga agccggtaca cccaataccg
    19081 gaggcatcat tggtcttggc gcggcgctgg agtatgtttc ggcgctgggg cttaataaca
    19141 tagccgagta tgaacagaat ctgatgcatt acgcgttatc acagttggaa tctgtaccgg
    19201 atctcactct ctatggccca caaaacaggc ttggcgttat tgcttttaat ctcggtaaac
    19261 accacgccta tgatgttggc agttttctcg ataattacgg cattgctgtg cgtaccggac
    19321 atcactgcgc aatgccatta atggcctatt acaacgtccc tgcgatgtgt cgggcatcgc
    19381 tggccatgta taacacccat gaagaagtgg atcgtctggt gaccggcctg caacgtattc
    19441 accgtctgct gggataacag ggaggcacta tggctttatt gccggataaa gaaaaattgc
    19501 tgcgtaattt tttacgctgc gccaactggg aagagaaata tctctacatt attgagttgg
    19561 gtcagcgtct gccagaatta cgtgacgaag acaaaagccc gcaaaatagc atccagggct
    19621 gccagagtca ggtgtggatt gtgatgcgtc aaaatgcgca gggaattatt gaattacagg
    19681 gcgacagcga tgcggccatt gtaaaagggc ttattgcggt cgtctttatt ctttacgatc
    19741 aaatgacgcc gcaggatatt gtcaatttcg atgtgcgccc gtggtttgaa aaaatggcgc
    19801 tcacccaaca tctcacccca tcacgctcac aaggtctgga agcgatgatt cgcgcaattc
    19861 gcgccaaagc cgctgcactt agctaaacta aagggacagc tttcatccgg ttggctcaca
    19921 ttgccgggtg gtcaatgttt acactggcct gcacaggagt gttttggttt caggtgaaca
    19981 taagcgccat ccgacgtttc agcgtgagtc tccggcaaat acaggaggtt tacaatgaaa
    20041 tgcgcgtctt tgcttacact cacgcttatc ggcgctttta gcgccatcca ggctgcctgg
    20101 gcggttgatt atccgctacc accaaccggc agtcgactgg ttgggcaaaa tcaaacgtat
    20161 acggtgcaag aaggggataa aaaccttcag gccatcgccc gacgttttga tactgcggca
    20221 atgttgatcc ttgaagccaa taacactatc gccccggtgc caaaacccgg tacgacgata
    20281 actattcctt cacaactatt attacctgat gcgccgcgtc aggggattat tgtaaacctt
    20341 gcagagctgc gcctttataa ttatccgccg ggagaaaata tcgtgcaggt ttatccgata
    20401 ggtattggtt tgcaggggct ggaaacgccg gtgatggaaa cgcgtgtggg gcagaaaatc
    20461 cctaacccaa cctggacgcc tacggcgggc attcgtcaac gttcgctgga acgtggcatt
    20521 aaattaccac cagtcgttcc tgccgggccc aataacccac taggacgtta cgcactgcgc
    20581 ctcgcgcatg gtaatggcga atacctcatt cacggcacca gtgcgccgga cagcgtcggt
    20641 ttgcgcgtca gttcagggtg tattcgcatg aatgcaccgg atattaaagc cttattctcc
    20701 agcgtgcgga cgggaacgcc ggtgaaagtg atcaacgaac cggtgaaata ttccgtggag
    20761 cctaacggga tgcgttatgt tgaagtacat cgaccactat cggcagaaga acagcagaac
    20821 gttcagacaa tgccatacac actgccagca ggctttacgc aatttaaaga caataagggt
    20881 gtagatcaga aattagtcga taaagcgttg tatcgtcggg cagggtatcc ggttgcggtg
    20941 agcagtggag caactcccgc agccagcaat gcgccttcag tagagtcagc gcagaatggt
    21001 gaaccagagc aagggaatat gttacgcgcg acgcagtagc ggtaaatggc aggcaaaaaa
    21061 aatggcgcac aatgtgcgcc atttttcact tcacaggtac tattacttgc ggtatttagt
    21121 agccatgttg tccagacgct ggttagcacg agctgcgtca tctttagcag cctgaacgtc
    21181 ggaacgcatt gcgttcacgt cgttgctcag ctggtcaact ttagcgttca gagtctgaac
    21241 gtcagaagac agctgatcga ttttagcgtt gctggagcaa cctgccagca gagtagaacc
    21301 caggattacc gcgcccagta ccagtttagt agctttcatt attaataccc tctagattga
    21361 gttaatctcc atgtagcgtt acaagtatta cacaaagttt tttatgttga gaatattttt
    21421 ttgatgggaa ggcacttatt tttgatcgtt cgctcaaaga agcatcgaac gctgggtttt
    21481 agataaaaaa attgagagaa aacaggatgc ttccatcgga ttcatcttag ataaagtgag
    21541 attatatagt gaaatccgat ttgatttttt attgcttctg gttatcgatt aaataaaaaa
    21601 agcgcccatc agggcgcttc gatatacaaa ttaattcaca aaagcaatat tacaggacgt
    21661 gaacagatgc ggtgttagta gtgccgctcg gtaccagtgc accagaaacc ataactacga
    21721 cgtcaccttt gtgtgccaga ccgctctgca gagccagttc tttacccaga cggtagaaat
    21781 catcagtaga agtgatctct ttaaccagct gcggcacaac gcctttgctc agtaccaact
    21841 gatgagccgt tttttcgttg gtggtcagtg ccaggatggt ggcatccggg aagtatttac
    21901 gtactgcgcg agcagattta ccgccctggg tagcaaccac gatcagcggg gcatccagtt
    21961 tttcagcagt ttcaacggca ccacggcata ccgcttcggt aatgcgcagt ttacggttgt
    22021 cattgttgaa ctcgagacgg ctgttcatca cgcggtcggt acgttcgcag atggtcgcca
    22081 tgatagaaac cgcttccagc gggtatttac cttttgcgga ttcaccagac agcatcactg
    22141 cgtcagtacc gtcgaggatg gcgtttgcaa cgtcaccggc ttctgcgcga gtcgggcgtg
    22201 ggtttttaat catggaatcc agcatctggg tcgcagtgat aacgacttta cgtgcacgga
    22261 tacatttttc gatcatcatc ttctgggcga agataacttc ttctaccggg atttctacac
    22321 ccaggtcgcc acgcgcaacc atgatgccgt cagaggcttc gaggatttcg tcgaagttgt
    22381 tgaggccttc ctggttttcg attttggaga tgatgtggat gttttcgccg ccgtgcgctt
    22441 tcaggtgctc acggatttcg ataacgtcag aacgcttacg aataaaggaa gcagcaacaa
    22501 agtctacgcc ttgttcgcaa ccaaagatca ggtcctgttt gtctttttca gccagtgctg
    22561 gcagggcaat ggaaacgcca ggcaggttca cacctttgtt ttcacccagg tcgccgttgt
    22621 tcagcacttt acagataact ttgttacctt caatggcggt aacttccata ccgatgagac
    22681 catcgtcaac cagtacggtg ttgccaacag acaggtcagt agtgaaacct tcatacgtta
    22741 ccgcaaccat ttcgctgttg ccgataacag atttatcagt ggtgaaagta aaggtctgac
    22801 ccgctttcag agaaacgtcg ttaccgcctt ccagtttcat ggtgcggatt tccggacctt
    22861 tggtatcaag caggatagcg gcggttttac cagttttgct catcacgttg cgcagattct
    22921 gaatgcgctg accgtgttct gcatagtcac catgagagaa gttcagacgc ataacgttca
    22981 tgccagcgtc cagcatttta gctaacatct cttcagattc ggttttcggt ccgatggtgc
    23041 aaacaatttt ggtctttttc atgacagtct tagtctttaa gttgagaagg atgggagaaa
    23101 cttgctttct gggcgcactg ccggaaagcg aatccggtat tacgaaagtg gtggtgcctc
    23161 gctctaagga taggtgacat cgaaaaagcg tgcagaggaa tgtgtgcttg tgtctcagcc
    23221 caacgtgagt gcctgttttt gagtcgttga gttctacagt ccaaagtgct gaaaccattc
    23281 aagagtcaat tggcgcgcat tatacgctga aattaatcca aaaggaaaac aaaaacagcg
    23341 tttcaaaaaa tatacaactt gttagacaat aaattgttat caaagattta acgttccagg
    23401 gaaaaggtta cgcaaaaact tcgctgtttg cgcaacgtct ggtgccgggg ttgtcactgt
    23461 gaagataagt gaaagttcat ccggtgtata aatacatcat cgctcaattg ctacagttaa
    23521 aataatttat cgcgttattt gtctgatttt gtttgatttt tgtttaattg gttagattga
    23581 tatatgctga tagttggcat gataaatagg tataaagaat gggaaatcgg acaaaagagg
    23641 atgaactgta ccgagaaatg tgcagagttg tcggtaaagt tgtgctggaa atgcgcgatc
    23701 tggggcagga accaaagcat attgttatcg caggcgtatt acgtacagca ttagccaata
    23761 aacgcattca gcgcagtgag ctggaaaagc aggcgatgga aaccgtcatt aacgcactgg
    23821 taaaatgacc attcaggatg aggcggtaag cttcatcctg atattcttgc ttcagttgcg
    23881 gtc
//
LOCUS       DAZGHF010000074        23867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGHF010000074 DAZGHF010000000
VERSION     DAZGHF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.074"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..417)
                     /locus_tag="ACHK3A_002989"
     CDS             complement(<1..417)
                     /locus_tag="ACHK3A_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO8741042.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYFGMVWAFFAALAAGWLASVSGISGFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFL"
     gene            675..1280
                     /gene="yahD"
                     /locus_tag="ACHK3A_002990"
     CDS             675..1280
                     /gene="yahD"
                     /locus_tag="ACHK3A_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO8741043.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLNIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            1320..2183
                     /gene="yahE"
                     /locus_tag="ACHK3A_002991"
     CDS             1320..2183
                     /gene="yahE"
                     /locus_tag="ACHK3A_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO8741044.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYCGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYLGLQCGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            2173..3720
                     /gene="fdrA"
                     /locus_tag="ACHK3A_002992"
     CDS             2173..3720
                     /gene="fdrA"
                     /locus_tag="ACHK3A_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO8741045.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLRNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGMKQEHLDLHTLNQPLIADVRA
                     RMQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSVKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            3720..5138
                     /gene="yahG"
                     /locus_tag="ACHK3A_002993"
     CDS             3720..5138
                     /gene="yahG"
                     /locus_tag="ACHK3A_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO8741046.1"
                     /translation="MSPSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPTAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            5164..5418
                     /locus_tag="ACHK3A_002994"
                     /pseudo
     CDS             5164..5418
                     /locus_tag="ACHK3A_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5468..6418
                     /gene="yahI"
                     /locus_tag="ACHK3A_002995"
     CDS             5468..6418
                     /gene="yahI"
                     /locus_tag="ACHK3A_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO8741047.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGVFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPQRIVEAPAIKALIQQGFVVIGAGGGGIPVVCSEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            6428..7810
                     /gene="yahJ"
                     /locus_tag="ACHK3A_002996"
     CDS             6428..7810
                     /gene="yahJ"
                     /locus_tag="ACHK3A_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO8741048.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTIHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENNQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVQIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGNVAG"
     gene            8106..8516
                     /locus_tag="ACHK3A_002997"
     CDS             8106..8516
                     /locus_tag="ACHK3A_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO8741049.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPLIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGCYSPEMDVIFEEYALIDVV"
     gene            8770..9753
                     /locus_tag="ACHK3A_002998"
     CDS             8770..9753
                     /locus_tag="ACHK3A_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8741050.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            9802..11286
                     /locus_tag="ACHK3A_002999"
     CDS             9802..11286
                     /locus_tag="ACHK3A_002999"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO8741051.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLSFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            11279..12250
                     /locus_tag="ACHK3A_003000"
     CDS             11279..12250
                     /locus_tag="ACHK3A_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8741052.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKMGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSILGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            12247..13203
                     /locus_tag="ACHK3A_003001"
     CDS             12247..13203
                     /locus_tag="ACHK3A_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8741053.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFTAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            13290..14339
                     /gene="yahK"
                     /locus_tag="ACHK3A_003002"
     CDS             13290..14339
                     /gene="yahK"
                     /locus_tag="ACHK3A_003002"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO8741054.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDI
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEHLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSELKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            14961..15206
                     /gene="yahM"
                     /locus_tag="ACHK3A_003003"
     CDS             14961..15206
                     /gene="yahM"
                     /locus_tag="ACHK3A_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO8741055.1"
                     /translation="MAVQLFKTLLNQIPLLSFLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(15223..15894)
                     /gene="yahN"
                     /locus_tag="ACHK3A_003004"
     CDS             complement(15223..15894)
                     /gene="yahN"
                     /locus_tag="ACHK3A_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO8741056.1"
                     /translation="MMKLLHLFMVEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            16041..16316
                     /gene="yahO"
                     /locus_tag="ACHK3A_003005"
     CDS             16041..16316
                     /gene="yahO"
                     /locus_tag="ACHK3A_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO8741057.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(16417..18003)
                     /gene="prpR"
                     /locus_tag="ACHK3A_003006"
     CDS             complement(16417..18003)
                     /gene="prpR"
                     /locus_tag="ACHK3A_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO8741058.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGHEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFAAALRQGLQESETMLVHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            18242..19132
                     /gene="prpB"
                     /locus_tag="ACHK3A_003007"
     CDS             18242..19132
                     /gene="prpB"
                     /locus_tag="ACHK3A_003007"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO8741059.1"
                     /translation="MYLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            19292..20461
                     /gene="prpC"
                     /locus_tag="ACHK3A_003008"
     CDS             19292..20461
                     /gene="prpC"
                     /locus_tag="ACHK3A_003008"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO8741060.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            20495..21946
                     /gene="prpD"
                     /locus_tag="ACHK3A_003009"
     CDS             20495..21946
                     /gene="prpD"
                     /locus_tag="ACHK3A_003009"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO8741061.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            21986..>23867
                     /gene="prpE"
                     /locus_tag="ACHK3A_003010"
     CDS             21986..>23867
                     /gene="prpE"
                     /locus_tag="ACHK3A_003010"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO8741062.1"
                     /translation="MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVATRIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AME"
BASE COUNT         5815 a         5926 c         6547 g         5579 t
ORIGIN      
        1 cagaaaaccc agtaatgtca ccgtccagtt catcccgctg gcaaaaaata gcgtcatgcc
       61 gagaaagcct ccgggaataa aagacagtaa accgaaacgt ccttgccaga ccaccaccgc
      121 cgagaagggt acagtcataa tcacgctcgc ccagaacccg gaaatcccac ttacagaggc
      181 cagccagccg gcagccagcg cggcgaaaaa ggcccacacc atcccgaaat aattaaccgc
      241 cagacttctg ataaaccccg atttaccgcc gcctgcggca taaaaactgc aaaaggcgac
      301 gaagccaatg gttcctaata attcccatcc ctgagagagc ccgacatgtg aagacactaa
      361 ctgccaaagc cctgcacaaa taccgacggt gacacccgtt gccgtgagtc cgttcatctt
      421 gttcccctct ggttagacat ggacgaaagc tactcttaca tgtgcagact atagagaaat
      481 aaaacctgat tgagtgaaag ggtatcgggt caaagaaaca aatattgcac taccgcacac
      541 tgcgaaaaga ttgttgaaca tcattcaaca aaaaggcgaa atacggcgaa ttaaatttaa
      601 gcgggttctt ttatcagatt atcgttgcta ttctcaattc atcgaaaaca gccacaaaaa
      661 ccaggggaat gtgaatgact ataaaaaatc tacctgccga ctatttattg gctgcgcaac
      721 agggtgatat cgataaagta aaaacctgcc ttgcgctggg tgtcgatatt aatacctgcg
      781 atcgtcaggg gaaaacggca attacgctgg caagtttata tcagcaatat gcttgcgttc
      841 aggcattaat tgatgccgga gcggatatta ataagcaaga tcatacctgt ttaaatcctt
      901 ttttaattag ctgtctgaac gatgatttaa cgctactacg aattatttta ccggctaaac
      961 ccgatcttaa ttgcgtcacc cgttttggcg gtgtcggcct gacgcctgcc tgtgaaaaag
     1021 gccatttaaa tattgtaaaa gagcttttgg cgcatacgga gattaacgtt aaccagacca
     1081 accatgttgg ctggacgccg ctactggaag cgattgtgct taatgatggc gggattaaac
     1141 agcaggcgat tgtccagtta ttgctggaac acggtgccag cccgcacatg acggataaat
     1201 atggcaaaac gccgctggaa ctggcgcggg aacgaggctt tgaagagatt gcgcagttac
     1261 tgattgccgc aggtgcataa accgggaggc ttgctatcaa cacaccagaa agacggtgtg
     1321 tgtgggcgct aactgcggat gctgattttc tggcgcagcg ggggcaagga caggttgaac
     1381 aggtctttgc cagagcggta aatatcgcac ttccggcccg ccagcagttg ctgacgctgc
     1441 tttgtgaaga gtacgacaat gcgccaaaca gttgtcggtt ggcactcact cactttgatg
     1501 gtctgttccg gcatggtgat aaggttcagt ttgacgatca aggcattacg gttggtcaac
     1561 atcttcatat agagatgagt cgttgtcggc gttggctgtc cccaaccttg caaatggccg
     1621 ctgtgaattt tcaccttatc gcctggcaac agtggcatga cattattcat cagcacctgg
     1681 gggaaaatga aaccctgttt aattattgcg gcgataatcc gttttatcag gcgttaaata
     1741 aagaattaca tattaaacga cgggcagtta ttcaggccgt aaacgataaa caaaatatcg
     1801 cctcagcagt cgccagtatg atgggcttag ggattggcct tacgccatca gccgacgatt
     1861 atttaacagg tctggcgctt attttattta tttccggaca tccggcggaa aaatacaaag
     1921 aggaatttta tctcggtctg caatgcggca gaaataatac cacgttatta agtgccataa
     1981 cgctggaagc cgcattacaa caacgctgcc gggaaaatat tcatcgtttt attcataaca
     2041 ttatttatga catccctggg aacgcaactc aggcaataga aaaaattaaa catattggct
     2101 ccagttccgg ctgcgacatg ctgtatggca tggccgatgg ttgtgcgctg agccaaacct
     2161 acggagggaa ttatgtcagt taaaatagtc attaaaccga atacctattt tgattctgtc
     2221 tcgctgatgt ctatctccac gcgtgcaaat aaactcgacg gcgtcgagca ggcatttgtg
     2281 gcgatggcga ccgaaatgaa caaaggcgtg ctgaggaatt taggactgct gacgccggaa
     2341 ctggagcagg cgaaaaacgg tgacctgatg attgtcatca atggtaaatc gggtgcggac
     2401 aacgagcagt tgctggtgga gattgaagaa ctgttcaaca gcaaagcgca aagtggctcg
     2461 cacgaggcgc gttacgccac tattgccagc gccaaaaagc atatcccgga aagtaacctg
     2521 gcggtgattt cggtcaacgg tctgtttgcc gctcgcgaag cgcgtcaggc gctgcaaaac
     2581 gatcttaacg tgatgctgtt ttccgataac gtctcagttg aagatgaact ggcgctcaaa
     2641 cagttggccc acgaaaaagg gctgctgatg atggggccag actgcggtac ggcgattatc
     2701 aacggcgcgg cgctctgctt tggtaacgcc gtgcgtcgcg gcaacatcgg cattgttggc
     2761 gcatccggca ccggtagcca ggaactgagc gtccgcattc atgaatttgg cggcggcgtt
     2821 tcgcaactga tcggcaccgg cgggcgggac ctgagcgaga aaatcggcgg cctgatgatg
     2881 ctcgacgcta tcgggatgct ggaaaacgat ccgcaaactg aaatcattgt gcttatctcc
     2941 aaaccgccag cgcctgcggt ggcccgcaaa gtgctggaac gcgcacgcgc ctgccgtaag
     3001 ccagtggtag tctgcttcct cggtcgtggc gaaacgccag tggatgagca ggggctacag
     3061 tttgctcgcg gcaccaaaga agcggcacta aaagcggtga tgctctccgg tatgaaacaa
     3121 gaacatctcg acttgcatac cctgaaccag ccgttgattg cggatgtgcg tgcgcgtatg
     3181 caaccgcagc agaaatacat tcgtggcctg ttctgcggcg gcacgctgtg tgacgaaacc
     3241 atgtttgcgg tgatggaaaa acatggcgat gtctacagca acattcagcc cgatcctgaa
     3301 ttccgcctgc aagatatcaa ccgcagcgtc aaacatacct tcctcgactt tggcgatgac
     3361 gattttacca acggcaagcc gcatccaatg attgacccga ccaaccgcat cagccggttg
     3421 atcgaagaag cgcgcgatcc agaagtggcg gtgatcgtga tggattttgt gctcgggttt
     3481 ggatcgcatg aagatccggt cggttccacc atcgaggcga tcaaagaggc gaaagcgatc
     3541 gccgctgccg aagggcgcga gttgatcatt ctcgcctatg tgctgggtac cgatctcgat
     3601 acgccatcgt tagaacaaca aagccagatg ttgcttgatg ccggagtgat tctggccagc
     3661 agcagcacca ataccggatt gctggcgcgt gaatttatct gcaaagggga ggaagcctga
     3721 tgagcccgtc actgtttagc caaccgttga acgttattaa cgtcggtatc gccatgttta
     3781 gcgatgatct gaaaaagcag catgtagaag tgacccaact cgactggacg ccgccggggc
     3841 agggcaatat gcaggtggtg caggcgctgg acaacattgc cgattccccg ctggcggaca
     3901 aaatcgccgc cgctaaccag caggcgctgg agcgcattat ccagtcgcat ccggtgctga
     3961 ttggttttga tcaggcgatt aacgtggtgc cgggtatgac gccgaaaacc attcttcacg
     4021 ccgggccgcc gatcacctgg gaaaaaatgt gtggcgcgat gaaaggcgcg gtcaccggag
     4081 cgctggtgtt cgaagggctg gcgaaagatc tcgatgaggc ggctgaactg gctgcctccg
     4141 gggagatcac cttctcgccg tgccacgagc acgactgcgt gggttcgatg gcgggtgtta
     4201 cctcggcctc gatgtttatg cacatcgtaa aaaacaaaac ctacggcaac atcgcttaca
     4261 ccaatatgag cgagcagatg gcgaagattt tgcgtatggg cgctaacgac cagagcgtga
     4321 ttgaccgcct caactggatg cgcgatgtgc aggggcccat actgcgcgac gcgatgaaaa
     4381 ttatcggcga aatcgatctg cgcttaatgc tggcgcaagc gctgcatatg ggcgatgagt
     4441 gccataaccg caataacgcc gggacgacgc tgctgattca ggcgctgacg ccagggatta
     4501 ttcaggcggg ctattccgtc gaacaacagc gggaagtgtt tgagtttgtc gccagcagcg
     4561 actacttctc cggcccgacg tggatggcga tgtgtaaagc ggcgatggat gcggcgcacg
     4621 gcattgaata cagcactgtg gtcaccacca tggcgcgtaa cggcgtcgag ttcggcctgc
     4681 gggtcagcgg cctgccgggg caatggttta ccggcccggc gcagcaggtg atcggcccga
     4741 tgtttgccgg ttataagccg gaagattcgg ggctggatat cggcgacagc gccatcactg
     4801 aaacctacgg cattggcgga tttgccatgg cgaccgcgcc tgcaatcgtt gcgctggtgg
     4861 gcggcacggt ggaagaggct atcgatttct cccgccagat gcgtgaaatc actctcggtg
     4921 aaaaccccaa cgtcaccatt ccgctgctcg gttttatggg ggtgccgacg gcaatcgaca
     4981 tcacccgcgt gggcagtagc ggcattctgc cggtgatcaa caccgccatc gcccataaag
     5041 atgcgggcgt cggcatgatt ggcgcgggca ttgtgcatcc accattcgcc tgcttcgaga
     5101 aagccattct tggctggtgc gaacgttacg gcgtctgagt tacatgttta tgccggatgc
     5161 gacatgaatg tcttatccgg cctacgaatg gcgcggaaat ctgtaggcct gataagcgtg
     5221 gcgcttcagg cattggccac cacagccgga tgcgacgtga acgccttatc cggcctacga
     5281 atggcacgga aatctgtagg cctgataagc gtagcgcatc aggcattggt caccacagcc
     5341 ggatgcggcg tgaacgcctt atccggccta cgagtggcgc aagaatctgt aggcctgata
     5401 agcgtagcgc atcaggcagt ctggcgttgg tcataacccc atcaccctct gtagcggaca
     5461 taaaaatatg aaagaacttg tggtcgttgc cattggtggc aacagcatta tcaaagataa
     5521 cgccagccag tcgattgagc atcaggcgga ggcggtgaaa gccgtcgccg atacggtgct
     5581 ggaaatgctg gcttccgatt acgacattgt gctgacccac ggtaacgggc cgcaggtcgg
     5641 gctggattta cgccgcgcgg agattgctca cgagcgcgaa gggctgccct taacgccgct
     5701 ggcaaactgt gtggcggata cgcaaggtgg cattggctat ctgatccagc aggcgctgaa
     5761 caaccggctg gcccgccacg gcgagaaaaa agccgtcacc gtggtgactc aggtggaagt
     5821 ggataaaaac gatccgggat ttgcccatcc caccaagcct atcggcgtat tcttcagtga
     5881 aagccagcgt gacgaattac aaaaggcaaa ccctgactgg cgttttgttg aagatgccgg
     5941 acggggctat cgccgcgtgg tcgcctcgcc ggaaccgcaa cgtattgtag aagcacctgc
     6001 cattaaggcc ctgatccaac aaggctttgt ggtgattggc gcgggcggcg gcggaattcc
     6061 tgtggtgtgc agtgaagcgg gagattacca aagcgtggac gcggttatcg acaaagatct
     6121 ctctaccgcg ctattggccc gtgaaattca cgccgacatt ctggtaatta ccaccggcgt
     6181 cgaaaaagtg tgtattcact ttggcaaacc gcagcagcag gcgctggatc gggtggatat
     6241 tgccaccatg acccgctata tgcaggaagg gcatttccca cccggcagca tgttgccaaa
     6301 aatcatcgcc agcctgacgt tcctggagca gggcggtaaa gaagtgatta tcaccacgcc
     6361 ggaatgcctg cctgcggcgc tgcgtggtga aacgggcacc catattatta aaacgtaagg
     6421 acgtaagatg aaagaaaaca atagccgccg tgagtttctg agccagagcg gtaagatggt
     6481 caccgccgcc gcgctgtttg gtacctctgt gccgctcgcc catgcggcag tctctggcac
     6541 aatccattgc gacgcgaata acaccatgaa aatcactgac ccgcactact atctcgacaa
     6601 cgtgttgctg gaaaccggtt ttgactacga aaatggcgtg gcggtgcaga cccgcacggc
     6661 gcgccagact gtggaaattc agaacggcaa aattgttgcc ctgcgcgaga acaatcagca
     6721 tccggacgcc acgctgccgc actatgacgc tggcggtaag ctgatgctgc ccaccacccg
     6781 cgacatgcat attcatctcg acaaaacctt ctatggcggg ccgtggcgct cgctcaatcg
     6841 tccggcaggc accaccatcc aggacatgat caaactcgag cagaaaatgc tgccggagtt
     6901 gcaaccgtac actcaggaaa gggcggaaaa actgattgat ttattgcagt cgaaaggcac
     6961 taccattgcc cgcagccact gcaatatcga accggtttcc ggcctgaaaa atctgcaaaa
     7021 tttgcaggcg gttctggcgc gacgtcaggc gggcttcgag tgtgaaattg tcgccttccc
     7081 gcagcacggt ttgctgctgt cgaaatcaga acccttaatg cgcgaagcga tgcaggcggg
     7141 ggcgcattac gtcggcgggc tggacccgac cagtgttgat ggcgcgatgg aaaaatccct
     7201 cgacaccatg ttccagattg cgctggacta cgacaaaggc gtcgatattc acctgcacga
     7261 aaccactccg gcaggcgtgg cagccatcaa ttatatggtt gaaacggtag agaaaacgcc
     7321 acagctgaag ggcaagctga ccatcagcca cgccttcgcg ttggcaacgc tcaacgaaca
     7381 acaggtagat gaactggcga accggatggc ggcgcagcaa atttctatcg cctcgacggt
     7441 gcagatcggc acgctacata tgccgcttaa acaactgcac gacaaaggcg taaaagtcat
     7501 gaccggaact gacagcgtta ttgaccactg gtcgccttac ggcctgggcg acatgctgga
     7561 aaaagccaat ctctacgcgc agctctatat tcgtcctaac gaacagaacc tctcccgttc
     7621 gctgttttta gctactggcg atatattgcc gctcaacgaa aaaggcgagc gtgtgtggcc
     7681 taaagctcag gatgacgcca gctttgtgct ggtggacgcc tcctgttccg ccgaggcggt
     7741 ggcgcgtatc tcgccgagaa ccgcaacgtt ccataaaggg caactggtgt gggggaatgt
     7801 ggcaggttga tgcgcatttg atatgtctgc aacggtgaac agtaagagat ttaagcccca
     7861 gggagtgggg cgggaatggt ttgttaatga atgatcctga actttataat ctcccggcat
     7921 aatttccagg ggattatttg agagaaatac acaaaagtga gttttaacaa aggtatttat
     7981 caagccataa atgtgacctg cttcggataa aaaagattaa acataggaaa accaatgata
     8041 ctcttgctga tggttaccaa cagtaattaa gatgtgattt tttccgtaat cgaaaaaata
     8101 ttgccatgac cataaaagaa aaattctcac aaaaatatcc ccatgcttcc ttttgtactt
     8161 ttggcgattc agcagcgttg gccgatcacc tcgcaacgtt gattgcgact ggtgttaaaa
     8221 cagcttcctg tggttcgctg gcgggatgta ttgaagacaa cgcatttccg ctgattggtg
     8281 agtataagat tgtagaaaat agccgtggtg agcctgtctg tgttatcaga gtgattggcc
     8341 tgcatttact gcgcttttct gatgtcaccg cagagcttgc ccgtaaggaa ggtgaaggtg
     8401 acctcagtct tgaatattgg cgtaatgaac accgtcgttt tttccaggca gaaggatgct
     8461 attcaccaga aatggacgtt atctttgaag aatatgccct tattgacgtt gtgtagataa
     8521 ctatttttct acgcgaaata agctcacaaa cataaagtat cgctaatttt gtttattttg
     8581 ctgtttttta cgtgatgatt gtcacactta ttgttgctgc ccgttttgtc catatttata
     8641 accaaatttt taatagctgc ctgaaatagg gctgtttatt ctgcgtaaaa atattcgaaa
     8701 attcttattc tctccagcgc gctaatttgg gcgaacactt cctgactacc ctgcaatgag
     8761 gctgaaatga tgaataaacg ttttgttatc aatatggtgt cttcgctgtt attaggcgct
     8821 gcgctaattt ctgcgccgct ccaggcagcg gaaaaagtcg tggtgaatat ctctaaagtc
     8881 gatggtatgc cgtggtttaa tcgcatgggc gaaggtgtgg ttgaggcggg taaagccttt
     8941 ggtgttaatg cgtcacaggt ggggccttca agtactgatg ctccgcaaca ggttaaaatt
     9001 attgaggatt taatcgcccg taaggttaat gcaattacta tcgtgccaaa cgatgccaac
     9061 gtgctggaac cggtattcaa aaaagcgcga gatgccggaa ttgtggttct gaccaacgaa
     9121 tctcccggtc agccaagtgc taactgggat atcgaaatca ttgataacga aaaatttgcc
     9181 gctgaatatg ttgagcatat ggcgaaacgc atggggggca agggcggcta tgtgatttac
     9241 gtgggtagtt tgaccgtgcc gcagcataac ttatgggctg acttgctggt gaaataccag
     9301 aaagagcatt acccggatat gcatgaagtt acccgccgca tgccggttgc cgagagtgtg
     9361 gatgactcac gtcgtaccac actggacctg atgaaaacct atcctgactt gaaagcggtt
     9421 gtctcctttg gttcaaacgg tccgattggc gcgggacgtg cagtgaaaga gaaacgggcg
     9481 aaaaataaag tcgcagtcta tggcatgatg atcccttcac aggcggcatc actgattaaa
     9541 agtggtgata tcaccgaagg gattacttac gatccggcct ctgcaggtta cgcactcgcg
     9601 gccgtcgcca gcacgttgct gaagggtgaa gagatcaaac ctggattaga aatgcagaat
     9661 ctcggtaagg ccgacgtaga tatggataag cgaattatcc gtttccataa ggtattactg
     9721 gtcaacaaag acaatatcga ctcactttat taatccattt cttcggggcg aaagccccga
     9781 ttcttattgc caggggatgc tatggaaacc ttcctttccc ttcgtcatat caacaaaacg
     9841 tttcatgcca cgcgagcgct gcgcgatgtt tctctcgact ttatgtcggg tgaggtgcac
     9901 tgccttgccg ggcaaaacgg ctgtggtaaa tcgacgttga ttaaaattat gtccggtgtt
     9961 tatcgcccgg atgagggggc ggaaattacg cttggtggga aaaactggtc aaagctgaca
    10021 cccgccgctt cggtggcgca ggggattcag gtgatttatc aggacctctc tttatttcct
    10081 aacctgagtg tctgggagaa tatcgccgtg aatcactatc accacggtct gtttgttaac
    10141 cgccgtcgtc tgcgtgaggt ggcgcaggcg gcaatgacca gtattaacgt cacattgcca
    10201 ctggatacgc tcgtttctga gctttcaatt gcccgctgtc agctggtggc aatttgccgt
    10261 gcactggcgc aggacgcacg gttgatcgtg atggatgaac caaccgcttc gctgacccat
    10321 caggaagtgc aagggttatt acaggtggtg catcaattgc gggaacgcgg gatctgcgtg
    10381 gtcttcgtca gtcatcgtct ggaagaagtg atggaagttt ccgaccgtat ttcagtgctg
    10441 aaagatggtg agctggtcgg gacctttccg gccgcagaga tgaccacaaa acaactcagt
    10501 tttctgatga ccggccagga gttcgaatat caggtacgag agttgtggca gggaaaatcc
    10561 agcaccccgg tgctggaggt gcgtaattta agtcgtcatg gggaatatca aaatattaac
    10621 ttgcgggtgg aagcgggcga agtggtgtcg attgttggcc tgcttggtgc ggggcgcacg
    10681 gaattatgtc tgagtctgtt tggtatgacc cgaccggatg ctggtgagat cctcatcaac
    10741 ggtcagccgg ttacactgca tagtaaccag gacgccatcc gccacggtat tggttatgtt
    10801 tcggaagatc gcatgtcgcg cggtttggtg atggcgcaat ctattgaaga caacatcatt
    10861 agcactgttt ttcacaaggt aaaagatcgc ttcggctttc tgagtgaagc taaagtgcgt
    10921 gacctggtgg acaggctaat caaggcattg accatcaaag tctctgaccc acatctcccg
    10981 gtgaatacgc tttccggggg taacgcgcag cgagtatcga tcgccaaatg gctggcgatt
    11041 gggccacgac tgttgattct cgatagcccg actgtcggtg ttgatatcgc caacaaagca
    11101 ggaatatacg gcattatcag cgatctggcg gctcatggca ttgcggtatt gatgatctgc
    11161 gatgaaattg aagaagcgtg gtatcaaagc caccggattt tggtaatgca aaaaggtcag
    11221 atcacccata gcttcctgcc tgacagcagt tctcaggcca ggattgcgga ggtggtaaat
    11281 ggctgaactg aaaaaacgcc atgaattctg gctggcatta ctgattgtgg tgctgtttgt
    11341 tggccttgcc tggcgcagcg acgagtttct gacattcggt aatttgtacg atctcgccaa
    11401 taactatgcc atgttgacta ttctcgcctg tggtttgttt gtggtgttaa tttccggtgg
    11461 gattgatatt tcgtttccgg caatgaccat cattgcgcaa tacggcatgg tgctgttgct
    11521 gcaaaaaatg ggtggcaact ttgctgtcgc gtttgccctg gcgggcggca tcggcatttt
    11581 acttggctta ataaacgcct tactggttaa tcgcctacgg gtgccgtcta tcatcatcac
    11641 tatctcgacg ctgaatattt tttatggcct gctgttatgg ttgagtaaag gtgtgtggct
    11701 gtacgacttt ccgccgtggt ttgagcaggg ggtaatgttg ttcaagtaca ccgatgctga
    11761 tggctatgac tatggccttg gtctgccgct gatcgccatg attacggtgg tgctgctgac
    11821 agcgtttatc atgaatttca ccagtgtagg gcgcaaaatt tatgcccttg gcgggaaccg
    11881 cgaatcagcc agtcgcatcg gctttagtgt gctgaaactg caacttttcg tctatggcta
    11941 tatgggattg atgtctggtg ctgcgggtgt agtgcagtcg tggacggtga tgactgtcgc
    12001 ccccgattct attctgggtt atgagctgac agtactggct gcggtggtgc ttggtggcac
    12061 tagtttgctc ggcgggcgcg gcacgttaac cggtactttg ctcggcgtgg tgttgttggc
    12121 agtaatgcaa aacgggctaa atttattggg agtctcgtct tactggcaaa cattgatcac
    12181 aggcatcatc atcgttgcca gcattagcgc cacggcgtgg agtcagcatc agaaccggag
    12241 tctgctatga aaaaatcgtg gcgtaataac gttgagtttt acttgatcgg cctgctggtg
    12301 ctaactgttg ccgcgttcag cattactatg ccggagattt tctggtcgat aagtaatttc
    12361 cagtccgtcg cttcgcaaat gcccgtgctg ggcattctgg cgttggctat ggcggtgacc
    12421 atgctttgcg gtggcattaa cttgtcaatt atagccactg ctaacgcctg ttcactggtg
    12481 atggcgtggg tggcgactca gtatccaccg ggtattgcta cggttgtcgc tacgctgctg
    12541 gcgggcgctg gcgcggcggt gataatcggt ttatgtaacg gcgtattgat tgctggtatt
    12601 cgagtgtcgc caattcttgc aacgcttggg atgatgacgt tgctcaaagg ggtaaatatt
    12661 ctggtgacag gcggtagcgc cattgccaat tatccctcat gggtgctgtg gttgaatcat
    12721 gcacagtggt tcggtattcc gctaccgatg tggttgttta ccgcagtggc gctggggctg
    12781 tggatattgc tggagaaaac gccgctcggt aaatccattt atctgattgg ttctaacgaa
    12841 cgagcaacac tttactccgg tattaacact cgtcgggtgc tgatatgggt gtatgtcatt
    12901 tccgccttgc tttgtgcggt agcggcattt ttgatgatgt cgaaactcaa ctcggctaag
    12961 gcctcttatg gcgaatctta cttgctagtg tcaatcctcg cggcagtact cggcggggtt
    13021 aatccggacg gcggcagcgg gcggataatt ggtatggtgc tggcgctgtt tcttctgcaa
    13081 attattgaaa gcggcttcaa tattctcggt ataagtccct acctgacgat ggccttatgg
    13141 ggaacgttgc tgctctgttt cattcaggct cgcggcatgt tggggctgga tcgggtggtt
    13201 taaatattgg ctatattcaa tggacgcgtt ttgccgcgat gacatatcag gcatcgccaa
    13261 atacacatag ctaatcagga gtaaacacaa tgaagatcaa agctgttggt gcatattcag
    13321 ctaaacaacc acttgaaccg atggatatca cccggcgtga accgggaccg catgatgtca
    13381 aaattgaaat cgcttactgt ggcgtctgcc attccgatat ccaccaggtc cgttccgagt
    13441 gggcggggac ggtttatccc tgcgtgccgg gccatgaaat tgtaggacgc gtggtagctg
    13501 ttggtgatca ggtagaaaaa tatgcgccgg gcgatctggt tggtgtcggc tgcattgtcg
    13561 acagttgtaa acattgcgaa gagtgtgaag acgggctgga aaactactgt gatcacatga
    13621 ccggcaccta taactcgccg acgccggacg aaccgggcca tactctgggc ggctactcac
    13681 aacagatcgt cgttcatgag cgatatgttc tgcgtattcg tcacccgcaa gagcacctgg
    13741 cggcggtggc tcctttgttg tgtgcaggga tcaccacata ctcgccgcta cgtcactggc
    13801 aggccgggcc gggtaaaaaa gtgggcgtgg tcggcatcgg cggtctggga catatgggga
    13861 tcaagctggc ccacgcgatg ggggcgcatg tggtggcatt taccacttct gagttaaaac
    13921 gcgaagcggc aaaagccctg ggggccgatg aagttgttaa ctcacgcaat gccgatgaga
    13981 tggcggctca tctgaagagt ttcgatttca ttttgaatac agtagctgcg ccacataatc
    14041 tcgacgattt taccaccttg ctgaagcgcg atggcaccat gacgttggtt ggtgcgcctg
    14101 cgacaccgca taaatcgccg gaagttttca acctgatcat gaaacgccgc gcgatagccg
    14161 gctctatgat tggcggcatt ccagaaacac aggagatgct cgatttttgc gccgaacatg
    14221 gcatcgtggc tgatatagag atgattcggg ccgatcaaat taatgaagcc tatgagcgaa
    14281 tgctgcgtgg tgatgtgaaa tatcgttttg ttatcgataa tcgcacacta acagactgaa
    14341 aaaattaata aacatcctgt ggtttaacat attaacttcg ctccacttaa ctttttagtt
    14401 aaggagagcg aaataatatc aaagtagtgg taaacctata gcgtaaattc agattgttaa
    14461 attaactcca ctactttaca cgttagttat gagcgatttc tgatagtgtc tggtttaatc
    14521 agagctttat tatctgcgac gtttattttt atttaataga gccacctgca agttgttatt
    14581 ccaccagaga gtgaatcgca aagcaggtaa tcatttcatc tgtatatctg tatgccaact
    14641 ggcattccat ctcttttttc gccgatattt tgttgtgatc ggcgacactt cgcttaaaaa
    14701 agcatcagca gtggtttcgc agctatgcgg tgtataaaaa atgatctcat gcagatgttt
    14761 tgtgaatgtg tgggttgaca ttcatatgaa aaaaatcata attccatcat gtttgtgtgg
    14821 ggtcttttct gtatcttacg catcgcactc aagcctgaca gaaaatatgc tgtaaggctc
    14881 atatcaaaaa ccgccattag ctcatcagga agagcagacg acaaccagga ttgttgtatg
    14941 gtacggggtt cgaggcctcg atggcagtcc agcttttcaa aactcttctg aatcagatcc
    15001 ccttattatc attcctgcaa agtggaacat tgccattgtt tggctattca ggacgggtca
    15061 gaccaatgaa aaaagcccac acaggggaga gtgggctgaa atgggaagct aaagactcaa
    15121 gtaaacttat cggaaataag gaccacgcat tacgggggct atcatcgccg atggtcggga
    15181 ttcggcaaat caggttgatt acgtagagag taaattatct gctcaccgct gcgtcacccc
    15241 ttcgtaaatc aggcgtagcg cgaatacacc aataattgca ccaataaccc gactggcaac
    15301 gcgttgcata cgcccataag cacgacgcac tgcgggcaaa gaaaacgcct gactaagaaa
    15361 aacgcgccag ataactgatg cgagcacaat ccccgcccag gccattaaac gtgcccatgt
    15421 tggtgtttcg gcatttaatg ttactgagaa aatactaata aaaaataaaa cggtttgcgg
    15481 gttagagaga tcggtaatta atccacggcg gaaaaagata taccaggggg cactaatcgg
    15541 ttgttgtagt gtgctcattt gcggtgttga ctggcggcgc atgctgcacc acgcaaacca
    15601 taagagataa gcgccgccga cgattctgat aagcgaaaaa atctcctcac actgcgtaat
    15661 tagtgttgca agaccaaaca aacccaaccc ggaataaaat gcatcgccca acgccacgcc
    15721 cagtccggtc agcacccctg cgcgtcgacc ggaagccagg ctggtttgta ctaccacaaa
    15781 gagattggct cccggattaa aaaaagtaat cacgaacagt cctacggtca gataaacggc
    15841 gtgcaaagga tccatagtga tttcaaccat aaataagtga agtaacttca tcatatttct
    15901 accaaaaata atcgggtgcg agagagatca caaagtgtct tatttccggt tactggcgtt
    15961 tatgccctga ctgaactaat tattaatcaa cccaataatg tgggtgggtg atagtgtgat
    16021 aacaactctg gagccgtaat atgaaaataa tctctaaaat gttagtcggt gcgttagcgt
    16081 ttgccgttac caatgtctat gccgcagaat taatgaccaa agcggaattt gaaaaggttg
    16141 catcgcaata tgaaaaaata ggtgatattt caaccagtaa tgaaatgtcg accgcagatg
    16201 cgaaagaaga tttgatcaaa aaagccgatg aaaaaggggc tgatgtgttg gtactgacct
    16261 ctggtcaaac tgacaataag atccacggca cggcaaatat ttataagaag aagtaattct
    16321 gaatcctatg taaacatccc cgatgcgtaa gtttatcggt gatcatcatc tattgaaatt
    16381 tatgccggat aaagcgttcg cgctgcattc ggcagttcag cttttcagcc gccgccagaa
    16441 cgtcgtccgg ctgataccta aataattcgc cgctgctgtt ttatcgccat taaatttctc
    16501 cagtgcctgt tgtgctgtca gcaagcctgg aatgggagtc ttcgccgact cgcgtgccag
    16561 ttccggcagt agcagctgca aaaattgcgg cgttaaatcc ggcgtcgatt ccacacttaa
    16621 aaacagcgcc agtcgttcca tcatattgcg cagttcacga atattgcccg gccagtcgta
    16681 gtgcaccagc atggtttcgc tttcctgtaa tccctggcgt aatgcggcag caaacggggc
    16741 ggagagcgcc gccagagaca ctttcaaaaa actttccgcc agcggcagaa tatccgccac
    16801 ccgctcgcgc agtggtggca attgcagacg caaaatactc agccgataaa acagatcacg
    16861 gcgaaactgc ccttgccgca tatcttcttc cagattgcag tgagtggcgc taatgactcg
    16921 cacatccacc ggaacaggct gatgcccgcc gacgcgggtg acctcttttt cttccagcac
    16981 ccgcagcagc cgagtctgca acggcagcgg catttcgcct atctcgtcaa gaaacagcgt
    17041 gccaccgtgg gcgatttcga acagcccggc gcgacctccg cgtcgcgagc cggtaaacgc
    17101 cccttcctca tggccaaaca gctccgcttc cagcagcgat tcggcaatcg ctccgcagtt
    17161 gacggcaaca aacggatgcg actttttgcc ctgtcgcgca tcgtggcggg caaaatattc
    17221 ccgatgaatc gcctgggccg ccagctcttt gcccgtcccc gtttccccct caatcaacac
    17281 cgccgcactg gagcgggcat acagcaaaat agtctgccgc acctgttcca tctgtggtga
    17341 ttgaccgagc atatcgccca gcacgtaacg agtacgcagg gcgttgcggg tggcatcgtg
    17401 agtgttatgg cgtaacgaca tgcgcgtcat atccagcgca tcgctgaatg cctggcgcac
    17461 ggtggcggcg gaatagataa aaattccggt cattccggct tcttctgcca gatcggtaat
    17521 cagccccgcg ccgaccaccg cttcggtgcc gttagctttc agctcgttaa tctgcccgcg
    17581 cgcgtcttct tcggtaatgt agctacgttg atcgaggcgc aaattaaagg ttttttgaaa
    17641 cgccaccagt gccggaatag tttcctgata agtgacaacg ccgatagaag aggtgagctt
    17701 tccggctttt gccagagcct gtaacacatc gtagccgctc ggtttaatca aaataaccgg
    17761 cactgacagg cgacttttaa gatatgcgcc gttagagcca gcagcgatga tggcgtcgca
    17821 gcgttcgttt gccagttttt tgcggatgta ggtcaccgct ttttcaaagc caagctgaat
    17881 aggggtgata ttcgccaggt gatcaaactc gagactgata tcgcgaaaca gctcgaacag
    17941 ccgcgttaca gataccgtcc agatcaccgg tttgtcgtca ttcagccgtg gtggatgtgc
    18001 catagcgcac cgcaaagtta agaaaccgaa tattgggttt agtcttgttt cataattgtt
    18061 gcaatgaaac gcggtgaaac attgcctgaa acgttaactg aaacgcatat ttgcgaatta
    18121 cttcatgact atattgctaa caaattgaaa ttaaacattt aattttattc agtcaattgt
    18181 ggcacacccc ttgctttgtg tttatcaacg caaataacaa gttgataaca aaggatgggc
    18241 tatgtatcta cactctccag gtaaagcgtt tcgcgctgca ctgtctaaag aaaatccatt
    18301 gcagattgtt ggcaccatca acgctaacca tgcgctgctg gcgcagcgtg ccggatatca
    18361 ggcaatttat ctctccggcg gtggcgtggc ggcagggtcg ctggggctgc ccgatctcgg
    18421 tatttctact cttgatgacg tgctgacaga tattcgccgt atcaccgacg tttgttcgct
    18481 gccgctgctg gtggatgcgg atatcggttt tggttcttct gcttttaacg tggcgcgcac
    18541 cgtgaaatcg atgattaaag ccggtgcggc aggattgcat attgaagatc aggttggtgc
    18601 gaaacgctgc ggtcatcgtc cgaataaagc gatcgtctcg aaagaagaga tggtggatcg
    18661 gatccgcgcg gcggtggatg cgaaaaccga tcctgatttt gtgatcatgg cgcgcaccga
    18721 tgcgctggcg gtagaggggc tggatgcggc gattgagcgt gcgcaggcct atgttgaagc
    18781 gggtgccgag atgttgttcc cggaggcaat taccgaactc gccatgtacc gccagtttgc
    18841 cgatgcggtg caggtgccga tcctcgccaa catcaccgaa tttggcgcaa caccgctgtt
    18901 taccaccgac gaattacgca gcgcccatgt cgcaatggcg ctgtacccac tttcagcgtt
    18961 ccgcgccatg aaccgcgccg ctgagcatgt ctacaacgtc ctgcgtcagg aagggacgca
    19021 gaaaagcgtc atcgacacca tgcagacccg caacgagctg tacgaaagca tcaactacta
    19081 ccagtacgaa gagaagctcg acgacctgtt tgcccgtggt caggtgaaat aaaaacgccc
    19141 gttggttgta ttcgacaacc gatgcctgat gcgccgctga cgcgacttat caggcctacg
    19201 aggtgaactg aactgtaggt cggataagac gcatagcgtc gcatccgaca acaatctcga
    19261 ccctacaaat gataacaatg acgaggataa tatgagcgac acaacgatcc tgcaaaacag
    19321 tacccatgtc attaaaccga aaaaatcggt ggcgctttct ggcgttccgg cgggcaatac
    19381 ggcgctctgc accgtgggta aaagcggcaa tgatttgcat taccgcggct acgacattct
    19441 tgatctggcg gaacattgcg aatttgaaga agtggcgcat ctgctgatcc acggcaaact
    19501 gccgacccgt gacgaactcg ccgcctacaa aacgaaactg aaagccctgc gcggtttacc
    19561 ggctaacgtg cgtaccgtgc tggaagcctt accggcggcg tcacacccga tggatgttat
    19621 gcgcaccggc gtttccgcgc tcggctgcac gctgccagaa aaagaggggc ataccgtctc
    19681 tggcgcgcgg gatattgccg acaaactgct ggcgtcactt agctcgattc ttctctactg
    19741 gtatcactac agccacaacg gcgaacgcat tcagccggaa accgatgacg actccatcgg
    19801 cggtcacttc ctgcatctgc tgcacggcga aaagccgtcg caaagctggg aaaaggcgat
    19861 gcatatttcg ctggtgctgt acgccgaaca cgagtttaac gcctccacct ttaccagccg
    19921 ggtgattgcg ggcaccggct ctgatatgta ttccgcgatt attggcgcga ttggtgcact
    19981 gcgcgggccg aagcacggcg gggcgaatga agtgtcgctg gagatccagc aacgctacga
    20041 aacgccggac gaagccgaag ccgatatccg caagcgggtg gaaaacaaag aagtggtcat
    20101 tggttttggt catccggttt ataccatcgc cgacccgcgt catcaggtga tcaaacgtgt
    20161 ggcgaagcag ctctcgcagg aaggcggctc gctgaagatg tacaacatcg ccgatcgcct
    20221 ggaaacggtg atgtgggaga gcaaaaagat gttccccaat ctcgactggt tctccgctgt
    20281 ttcctacaac atgatgggtg ttcccaccga gatgttcaca ccactgtttg ttatcgcccg
    20341 cgtcactggc tgggcggcgc acattatcga acaacgtcag gacaacaaaa ttatccgtcc
    20401 ttccgccaat tatgttggac cggaagaccg ccagtttgtc gcgctggata agcgccagta
    20461 aacctctacg aataacaata aggaaacgta cccaatgtca gctcaaatca acaacatccg
    20521 cccggaattt gatcgtgaaa tcgttgatat cgtcgattac gtgatgaact acgaaatcag
    20581 ctccaaagta gcctacgaca ccgcacatta ctgcctgctc gacacgctcg gctgcggtct
    20641 tgaagctctc gaatacccag cctgtaaaaa actgctgggg ccaattgtcc ccggcaccgt
    20701 cgtacctaac ggcgtgcgcg tccccggaac tcagttccag ctcgaccccg tccaggcggc
    20761 atttaacatc ggcgcgatga tccgttggct cgatttcaac gatacctggc tggcggcgga
    20821 gtggggccat ccttccgaca acctcggcgg cattctggca acggcagact ggctttcgcg
    20881 caacgcggtc gctagcggca aagcgccgtt gaccatgaag caggtgctga ccgcaatgat
    20941 caaagcccat gaaattcagg gctgcatcgc gctggaaaac tcctttaacc gcgtcggcct
    21001 cgaccacgtt ctgttagtga aagtggcttc caccgccgtg gtcgccgaaa tgctcggcct
    21061 gacccgcgag gaaattctca acgccgtttc gctggcgtgg gtggacggtc agtcgctgcg
    21121 cacctatcgc catgcgccga acaccggcac gcgtaaatcc tgggcggcgg gcgatgccac
    21181 ttcccgcgcg gtacgtctgg cactgatggc gaaaacgggc gaaatgggtt atccgtcagc
    21241 cctgactgcg ccagtatggg gtttctacga cgtctccttt aaaggtgagt cattccgctt
    21301 ccagcgtccg tacggttcct acgtcatgga aaatgtgctg ttcaaaatct ccttcccggc
    21361 ggagttccac tcccagacgg cagttgaagc ggcgatgacg ctctatgaac agatgcaggc
    21421 agcaggcaaa acggcggcag atatcgaaaa agtgaccatt cgcacccacg aagcctgtat
    21481 tcgcatcatc gacaaaaaag ggccgctcaa taacccggca gaccgcgacc actgcattca
    21541 gtacatggtg gcgatcccgc tgctgttcgg acgcttaacg gcggcagatt acgaggacaa
    21601 cgttgcgcaa gataaacgca tcgacgccct gcgcgagaag atcaattgct ttgaagatcc
    21661 ggcgtttacc gctgactacc acgacccgga aaaacgcgcc atcgccaatg ccataaccct
    21721 tgagttcacc gacggcacac gatttgaaga agtggtggtg gagtacccaa ttggtcatgc
    21781 tcgccgccgt caggatggca ttccgaagct ggtcgataaa ttcaaaatca atctcgcgcg
    21841 ccagttcccg actcgccagc agcagcgcat tctggaggtt tctctcgaca gaactcgcct
    21901 ggaacagatg ccggtcaatg agtatctcga cctgtacgtc atttaagtaa acggcggtaa
    21961 ggcgtaagtt caacaggaga gcattatgtc ttttagcgaa ttttatcagc gttcgattaa
    22021 cgaaccggag aagttctggg ccgagcaggc ccggcgtatt gactggcaga cgccctttac
    22081 gcaaacgctc gaccacagca acccgccgtt tgcccgttgg ttttgtgaag gccgaaccaa
    22141 cttgtgtcac aacgctatcg atcgctggct ggagaaacag ccagaggcgc tggcattgat
    22201 tgccgtctct tcggaaacag aggaagagcg tacctttacc ttccgccagt tacatgacga
    22261 agtgaatgcg gtggcgtcaa tgctgcgctc actgggcgtg cagcgtggcg atcgggtgct
    22321 ggtgtatatg ccgatgattg ccgaagcgca tattaccctg ctggcctgcg cgcgcattgg
    22381 tgctattcac tcggtggtgt ttgggggatt tgcttcgcac agcgtggcaa cgcgaattga
    22441 tgacgctaaa ccggtgctga ttgtctcggc tgatgccggg gcgcgcggcg gtaaaatcat
    22501 tccctataaa aaattgctcg acgatgcgat aagtcaggca cagcatcagc cgcgtcacgt
    22561 tttactggtg gatcgcgggc tggcgaaaat ggcacgcgtc agcgggcggg atgtcgattt
    22621 cgcgtcgttg cgccatcaac acatcggcgc gcgggtaccg gtggcgtggc tggaatccaa
    22681 cgaaacctcc tgcattctct acacctccgg cacgaccggc aaacctaaag gcgtgcaacg
    22741 tgacgttggc ggatatgcgg tggcgctggc gacctcgatg gacaccattt ttggcggcaa
    22801 agcgggcggt gtgttctttt gtgcttcgga tatcggctgg gtggtggggc attcgtatat
    22861 cgtctacgcg ccgctgctgg cggggatggc gactatcgtt tacgaaggat taccgacctg
    22921 gccggactgc ggcgtgtggt ggaaaatcgt cgagaaatat caggttagcc gcatgttctc
    22981 agcgccgacc gccattcgcg tgctgaaaaa attccctacc gctgaaattc gcaaacacga
    23041 tctctcgtcg ctggaagtgc tctatctggc gggggagccg ctggacgagc caaccgccag
    23101 ttgggtgagc aatacgctgg atgtgccggt gatcgacaat tactggcaga ccgaatccgg
    23161 ctggccgatt atggcgattg ctcgcggtct ggacgacagg ccgacgcgtc tgggaagccc
    23221 cggcgtgccg atgtatggct ataacgtgca gttgctcaat gaagtcaccg gcgaaccgtg
    23281 cggcgtcaac gagaagggga tgctggtggt ggaagggccg ctgccgccgg ggtgtattca
    23341 gaccatctgg ggcgatgacg accgctttgt gaagacgtac tggtcgctgt tttcccgtcc
    23401 ggtgtatgcc acctttgact ggggcatccg cgatgctgac ggttatcact ttattctcgg
    23461 gcgcactgac gatgtgatta acgttgccgg gcatcggctg gggacgcgcg agattgaaga
    23521 gagtatctcc agtcatccgg gcgttgccga agtggcggtg gttggggtga aagatgcgct
    23581 gaaagggcag gtggcggtgg cgtttgtcat tccgaaagag agcgacagtc tggaagaccg
    23641 cgaggtggcg cactcgcaag agaaggcgat tatggcgctg gtggacagcc agattggcaa
    23701 ctttggccgc ccggcgcacg tctggtttgt ctcgcaattg ccgaaaacgc gatccggaaa
    23761 aatgctgcgc cgcacgatcc aggcgatttg cgaaggacgc gatcctgggg atctgacgac
    23821 cattgatgat ccggcgtcgt tggatcagat ccgccaggcg atggaag
//
LOCUS       DAZGHF010000075        22906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGHF010000075 DAZGHF010000000
VERSION     DAZGHF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.075"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..71)
                     /locus_tag="ACHK3A_003011"
     CDS             complement(<1..71)
                     /locus_tag="ACHK3A_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741063.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEK"
     gene            complement(147..764)
                     /gene="yncG"
                     /locus_tag="ACHK3A_003012"
     CDS             complement(147..764)
                     /gene="yncG"
                     /locus_tag="ACHK3A_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO8741064.1"
                     /translation="MIKVHGVPGWGTTISEMMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKNNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFHDNAQNISSIAEAVCQLPKLQEVLKRNEII"
     gene            1031..2530
                     /gene="ansP"
                     /locus_tag="ACHK3A_003013"
     CDS             1031..2530
                     /gene="ansP"
                     /locus_tag="ACHK3A_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO8741065.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2643..3704)
                     /gene="yncE"
                     /locus_tag="ACHK3A_003014"
     CDS             complement(2643..3704)
                     /gene="yncE"
                     /locus_tag="ACHK3A_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO8741066.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3946..6048
                     /gene="pqqU"
                     /locus_tag="ACHK3A_003015"
     CDS             3946..6048
                     /gene="pqqU"
                     /locus_tag="ACHK3A_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO8741067.1"
                     /translation="MKIFSVRQTVFPALLVLSPTVFAADEQTMIVSAAPQVISELDTP
                     AAVSVVDGEEMRLATPRINLSESLAGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMSPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            complement(6084..6749)
                     /gene="mcbR"
                     /locus_tag="ACHK3A_003016"
     CDS             complement(6084..6749)
                     /gene="mcbR"
                     /locus_tag="ACHK3A_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO8741068.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6947..7984)
                     /gene="curA"
                     /locus_tag="ACHK3A_003017"
     CDS             complement(6947..7984)
                     /gene="curA"
                     /locus_tag="ACHK3A_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO8741069.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDIATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGVEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIQEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAVDD"
     gene            8165..8680
                     /gene="mddA"
                     /locus_tag="ACHK3A_003018"
     CDS             8165..8680
                     /gene="mddA"
                     /locus_tag="ACHK3A_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO8741070.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERSEPDAI"
     gene            8680..9129
                     /gene="ydcZ"
                     /locus_tag="ACHK3A_003019"
     CDS             8680..9129
                     /gene="ydcZ"
                     /locus_tag="ACHK3A_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8741071.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSNVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(9130..9363)
                     /gene="ydcY"
                     /locus_tag="ACHK3A_003020"
     CDS             complement(9130..9363)
                     /gene="ydcY"
                     /locus_tag="ACHK3A_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO8741072.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREERYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9449..9622)
                     /gene="ortT"
                     /locus_tag="ACHK3A_003021"
     CDS             complement(9449..9622)
                     /gene="ortT"
                     /locus_tag="ACHK3A_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO8741073.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9817..9912
                     /gene="yncL"
                     /locus_tag="ACHK3A_003022"
     CDS             9817..9912
                     /gene="yncL"
                     /locus_tag="ACHK3A_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO8741074.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(10009..11433)
                     /gene="patD"
                     /locus_tag="ACHK3A_003023"
     CDS             complement(10009..11433)
                     /gene="patD"
                     /locus_tag="ACHK3A_003023"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO8741075.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(11455..12249)
                     /gene="ydcV"
                     /locus_tag="ACHK3A_003024"
     CDS             complement(11455..12249)
                     /gene="ydcV"
                     /locus_tag="ACHK3A_003024"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8741076.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAVYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(12239..13180)
                     /gene="ydcU"
                     /locus_tag="ACHK3A_003025"
     CDS             complement(12239..13180)
                     /gene="ydcU"
                     /locus_tag="ACHK3A_003025"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8741077.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWGNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLIMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(13181..14194)
                     /gene="ydcT"
                     /locus_tag="ACHK3A_003026"
     CDS             complement(13181..14194)
                     /gene="ydcT"
                     /locus_tag="ACHK3A_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8741078.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKSASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVDKKQRYAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDALMADKLCGMTGSFALRPEHIRFNIPGEVQANGT
                     IQAVQYQGAATRFELRLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(14212..15357)
                     /gene="ydcS"
                     /locus_tag="ACHK3A_003027"
     CDS             complement(14212..15357)
                     /gene="ydcS"
                     /locus_tag="ACHK3A_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO8741079.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGIRDPYQLTEEQY
                     QAVLKVMRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMSWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(15602..17008)
                     /gene="ydcR"
                     /locus_tag="ACHK3A_003028"
     CDS             complement(15602..17008)
                     /gene="ydcR"
                     /locus_tag="ACHK3A_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO8741080.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGHIIARPQSGYYVATQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(17087..17503)
                     /gene="hicB"
                     /locus_tag="ACHK3A_003029"
     CDS             complement(17087..17503)
                     /gene="hicB"
                     /locus_tag="ACHK3A_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO8741081.1"
                     /translation="MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(17547..17723)
                     /gene="hicA"
                     /locus_tag="ACHK3A_003030"
     CDS             complement(17547..17723)
                     /gene="hicA"
                     /locus_tag="ACHK3A_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO8741082.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIK
                     EPLRKAILKQLGLN"
     gene            17945..18175
                     /gene="yncJ"
                     /locus_tag="ACHK3A_003031"
     CDS             17945..18175
                     /gene="yncJ"
                     /locus_tag="ACHK3A_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HFO8741083.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGHR"
     gene            complement(18267..20228)
                     /gene="rlhA"
                     /locus_tag="ACHK3A_003032"
     CDS             complement(18267..20228)
                     /gene="rlhA"
                     /locus_tag="ACHK3A_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO8741084.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(20301..20837)
                     /gene="sutR"
                     /locus_tag="ACHK3A_003033"
     CDS             complement(20301..20837)
                     /gene="sutR"
                     /locus_tag="ACHK3A_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO8741085.1"
                     /translation="MENLTRFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQVMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHVYR
                     NGGEQIVHFHSLIHYPRS"
     gene            20890..22101
                     /gene="ydcO"
                     /locus_tag="ACHK3A_003034"
     CDS             20890..22101
                     /gene="ydcO"
                     /locus_tag="ACHK3A_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO8741086.1"
                     /translation="MLRYLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDDQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGVVIVIAQGDVVTTDVVFKPVLPTYISPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            complement(22136..22804)
                     /gene="ydcL"
                     /locus_tag="ACHK3A_003035"
     CDS             complement(22136..22804)
                     /gene="ydcL"
                     /locus_tag="ACHK3A_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO8741087.1"
                     /translation="MRTTSIAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPVTYYPVPKPSTQVGQKVLDKILSYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT         5596 a         5861 c         5593 g         5856 t
ORIGIN      
        1 ttcttttcag aggaaatgaa ataaacaagc tgaatgaaag ggagagtgat ataaattaaa
       61 aaacataaca tgaatctaat taattacgtc catcattgtg gataaagtta ctgaataata
      121 aataacataa tcttctggta tcccattcaa ataatttcat tcctttttaa tacttcttgt
      181 aatttcggta actgacatac agcttcagcg atcgagctga tattctgcgc attatcgtga
      241 aaccattcat gccccggccc ccatgtgcgc atggtgcaaa gataacagtc caccagcgtt
      301 agttgctcac caaacgcata aggttcagcg gtcaattgtg aattcagcca gatataaagc
      361 gatttacgat attcaataac attattcttt aactgttcgg gtgcgtcagg agcccaacgc
      421 tcagggtaat cggcgaaagt gaatgttgga tagacattag caaccagcca gaccaatagc
      481 cgttgaaact gctgacgttc ggcgcgccca accggtggcg caaggtccgg gcgacgatcg
      541 agaaccatca atgcaatcgc cgctgtctcc gtcataatct cgtcattttc cagcgccagt
      601 gtcggaatct gacacaacgg gttcagggtt ttcaataact cgcgtgaagt tccctcgtgg
      661 tcaaaaccac tgacatcaac gaactgataa ggaatatcag ccagtgttag catcatctca
      721 ctgattgtag tgccccatcc gggtacgcca tgcaccttaa tcatatgccc ctcacaatct
      781 tttaaagatt aagtctagga cagcacacag gttaaccagc aaaagtattt gtcatttttt
      841 cgtcagttgt atgaatcccc ttcagaattg taaggggatg ttttgcatcg tttatcgata
      901 acgggtagga tgctgcgccg atagttttat acccataaag aataatggtg ataactatca
      961 tcgccaggat gaataaacat tgttcatggc aacttatatg actttttcat taaagcaatc
     1021 agggagagca atgagtaaac acgacaccga cacttcagat cagcacgccg cgaaacgccg
     1081 ctggcttaat gcccacgaag aggggtatca caaagcgatg ggcaatcgcc aggtgcagat
     1141 gatcgccatt ggcggcgcga ttggtacggg tttgttttta ggcgcgggag cccgactgca
     1201 aatggcgggg ccagcactgg cactggtcta tttaatctgc ggcttgtttt cgttttttat
     1261 tctgcgtgca ttgggtgagc tggtgctaca ccgcccttcc agtggcagtt ttgtctctta
     1321 tgcccgtgag tttttgggtg agaaagccgc ttatgttgca ggctggatgt acttcatcaa
     1381 ctgggcgatg acggggattg tcgatattac cgccgttgca ctgtatatgc attactgggg
     1441 cgcatttggt ggtgtaccgc agtgggtctt tgcgctcgct gcgctgacca tcgttggcac
     1501 catgaatatg attggtgtga aatggtttgc cgagatggag ttctggtttg cgcttattaa
     1561 agtgctcgcc attgtgacct ttttggtcgt gggtacagtg ttcctcggta gtggtcagcc
     1621 gctggatggc aacaccactg gctttcattt aattaccgat aacggcggct tcttccccca
     1681 cggtttgcta cctgcgctgg tgttgattca gggcgtagtg tttgcttttg cctccattga
     1741 aatggtgggt acagctgccg gagaatgtaa agatccgcag accatggtgc ctaaagcgat
     1801 taacagtgtg atttggcgta ttggcctgtt ttacgtcggc tccgtggtgt tgctggttat
     1861 gttattgccg tggagcgcgt atcaggcggg gcaaagtccg ttcgtgacgt ttttctctaa
     1921 actgggtgtg ccgtacatcg gcagcattat gaacattgtg gtgctgaccg ctgccctctc
     1981 cagcctgaac tcaggtctgt actgcaccgg acgtattctg cgctcgatgg cgatgggcgg
     2041 ttccgcaccg agttttatgg cgaaaatgag tcgtcagcat gtgccgtatg ccgggattct
     2101 ggcgacactg gttgtgtatg tcgtcggcgt attcctcaac tatctggtgc cgtcgcgcgt
     2161 atttgagatt gtgttgaact tcgcgtcgct gggaatcatc gcttcatggg cgtttatcat
     2221 cgtgtgccag atgcgcctgc gtaaagcgat taaagaaggc aaagcagcgg atgtcagttt
     2281 taaactgcct ggcgcgccct tcacttcctg gctgacatta ctgtttttgc tgagcgtcct
     2341 tgtgctgatg gcgttcgatt acccgaacgg gacttatact atcgcggcgc tgccgattat
     2401 cggtattctg ctggttatag gctggtttgg tgtgcgcaaa cgcgttgctg aaattcacag
     2461 cactgcgcca gtcgtcgagg aagatgaaga aaaacaggaa attgtgttta agcctgaaac
     2521 ggcgagctaa ttgcaaaatt gcccggctac gtgccgggca aattcaaatt actgacgaag
     2581 gtgccggata cggcgtgata aaagtcaggc aagtctctcc attttcaccc tcgccatatc
     2641 tattacagcg caatacgaat cacatcgtcc ggctgggtag cttcctgctg tttagtggat
     2701 ttttgtttca cactgacata cagcgttttg ccatcggcag acagtgccag gctgttcgga
     2761 tgagtcggcg tatcgaacgt tttcaccact ttatagcttt tcgcgtcaat cacactgact
     2821 ttacctgcct gacgatgcgt cacgtaggct tcattacgcg cggggttaaa cagcacagcc
     2881 agtgattccg gtgccgcaac cttcgccaga atattgccat tacgggtatc gaccactaac
     2941 acttctgccg ctttagagtc ggtgataaat gcacgctgcc ttgcggtatc aaggctgatg
     3001 ttgataaaga agtgctcttt gccgtcatcc agcagctttt tacggctgag gattttattg
     3061 tcggcggtgt cgatggtaat caattcgccg tcagcgttag tggtgtaaag acgtttgcct
     3121 ttgctatcca gcgctaaacc ggtactcatt ttaccggtgt tctggatggc ggttttcagt
     3181 ttgatattct cgccatcaac gacccaaatc acgctctctt taccaatacc actgatgtaa
     3241 acggtgttcg tggcatcgtc tgccaccagc tcacgcggtt gcagcgggcg cacctcttca
     3301 gtgcgcttac gatcatccag caccagacgg cctttcactt cgcccgtttt ggcatctatc
     3361 gccgtgaccg cgctgtttac agtgttacca aaccacaacg tctgagtcgt gttattgatg
     3421 gtagcaccaa acggcttgag atcgttatgg atcgcctgcg tcacttccag agtaaccgga
     3481 tcaagacgat aaaccacgcc gcctttatcc agtttgcggc tttgcgaagt ggcgagccac
     3541 agcgcgtttt cttgctggct ataagccatt tcgtaggcac ctttacctac cgctttacgc
     3601 agcatttctt ctgcggcctg cgtactgaat gatgaagcaa caagcaatga acctaacagt
     3661 aatgaaccac gcaggcgcga tgaaaacaga tgacgtaaat gcatgacgac tccctttgat
     3721 gaaccgatat ttttgctgct tcacaatgct ccgctaagcg taatcatcgt tacgctcttg
     3781 ttataaatgg gaatggtaat cattattttc tcaaatgtgg agcatttctt gccgttacga
     3841 ctacaatttt ctcaattact taacatatcg tgccgtttcg ttttccataa atttacaatt
     3901 ttattaccat gacggtgttt tcccattctt aacctggctt ttttcatgaa gattttttcc
     3961 gtccgacaga ccgtttttcc cgcgctgctt gtcctttccc ccaccgtttt tgccgctgat
     4021 gaacagacca tgattgtcag tgccgcgccg caggtgattt cagaactgga taccccagca
     4081 gccgtgagcg tggtggatgg cgaggagatg cgcctggcaa caccgcgcat taacttgtct
     4141 gaatcactgg ctggcgtgcc tggtttgcag gtacaaaacc ggcagaacta tgcgcaagat
     4201 ttacagctgt cgattcgcgg atttggctcc cgctccactt acggaattcg cggtattcgc
     4261 ctgtatgttg acggtattcc cgctaccatg cccgacggac aagggcagac atccaatatc
     4321 gatttaagca gtgtgcaaaa tgtggaagtg ctgcgtggcc ccttctctgc tctgtatggc
     4381 aacgcgtctg gcggtgtaat gaatgtcatc acccagaccg gacaacagcc accaaccatt
     4441 gaagccagta gttactacgg cagttttggc agctggcgct atgggctgaa agcaacgggc
     4501 gcaacgggag acggcacaca gccaggcgat gtcgattaca ccgtctcaac cacgcgcttt
     4561 acgacccacg gctatcgtga tcatagtggc gcacagaaaa atttagccaa tgccaaactg
     4621 ggcgttcgca ttgatgacgc cagcaaatta agcctgattt tcaatagtgt ggatatcaaa
     4681 gcagatgacc caggtgggct aaccgaagca gaatggaagg cgaatccaca acaagcgccc
     4741 cgtgcagaac agtacgacac gcgaaaaacc atcaagcaaa ctcaggctgg gttgcgctat
     4801 gaacgtagcc tgagcgcgca agatgatatg agtgtaatga tgtatgccgg agagcgagaa
     4861 acgacccagt accagtcaat ccccatgtcc ccgcaactta acccgtcaca tgcgggcggc
     4921 gtgattaccc tgcaacgcca ttaccagggg atagacagcc gctggacaca ccgtggtgaa
     4981 ttgggcgttc cagtcacgtt cacaaccggc ctgaactacg aaaacatgag tgaaaaccgc
     5041 aagggttaca ataacttccg cctgaatcgc ggcgtgccgg aatacgggca aaaaggtgag
     5101 ttgcgccgcg acgaacgcaa tttgatgtgg aacgtcgatc cctatttaca aacccagtgg
     5161 caactgaccg acaaactttc gctggatgct ggcgtgcgct acagctctgt gtggtttgat
     5221 tccaatgatc attacgttac tccgggtaac ggcgacgaca gcggtgatgc cagttatcac
     5281 aaatggctac cagccggatc gttaaaatat gcaatgaccg atgcctggaa tgtctatctg
     5341 gcagccgggc gcggttttga aacgccgacg attaatgagc tgtcttatcg cgctgatggg
     5401 caaagcggta tgaactttgg tttaaaaccc tccaccaacg atacaattga gatcggcagt
     5461 aaaacgcgta ttggtgatgg gctgcttagt ctcgcattgt tccagaccga caccgatgat
     5521 gaaattgttg tcgatagcag tagcggtggg cgtacgactt acaaaaatgc cggaaagacc
     5581 cgtcgtcaag gcgctgaact ggcatgggat caacgtttcg cgggagattt tcgcgtaaaa
     5641 gcgtcctgga cctggcttga tgcgacctat cgcagcaatg tttgcaatga acaggattgt
     5701 aacggtaacc ggatgccagg gatcgcccgt aatatgggct ttgcgtcgat aggttatgta
     5761 ccggaagatg gctggtatgc aggtacggaa gcgcgttata tgggcgatat tatggcagat
     5821 gatgaaaata cggccaaagc gccgtcttat actctcgtcg gcttattcac cgggtataaa
     5881 tacaattacc acaatttaac tgtggattta tttggtcgtg tcgataattt attcgataaa
     5941 gaatacgttg gctctgtcat tgtcaatgag tcaaacgggc gatattacga acctgcgccc
     6001 gggcgaaatt atggtgtcgg catgaatatt gcgtggcgat ttgagtaatt accttgatgc
     6061 ccggtatttg ccgggcattt aatttaacga ttgtattgct ggtataaaat agcaatattt
     6121 tgttgcataa tttcagcaag acaatgtctg catccctctt tgtcttttgc ttttaacgcg
     6181 gcgagtaata gacgatagtt ttctatacgc tcccgtaatt ccgctggatt aatcgcttca
     6241 tagagataat gtaaaccagg tcccatcctg acccacagtt gctcaatcat ctcacacagg
     6301 atgggcatat ttgaacgatg ataaatcgct aagcgaaata gcctgttcgc attaatgatt
     6361 tgttccatgt cgcccttttc ttgcgcctgc tgtaatttct cgagcaattc ctcgagttcc
     6421 gccaggtctt gcggggtgag gttttcaaca gccagagtaa ctgccattaa ttccagctcg
     6481 tagcggatcc gattgatttc gtctaattga cgtttcccca cttccggaac tgtaaatgct
     6541 tgcgctggtg cgacagaaag cgcattcacc gaaaccagac gtaataatgc ttcacgcaca
     6601 ggtgtaatac tcatacctaa ttgctccgcc agatttttag taatcaggcg tgcgccaggt
     6661 tttaatgcgc caatactaag ctgatgtttc aggtcgttct caacctgcag agtcaaactg
     6721 acatgtttca ttttttccgt tccaggcatt ttaattcctt cgcgtgtttt ctgtccaacc
     6781 ctgcctgcat ggcagaagga gttcacctgg tgtattaaag tgaataatca taaaccactc
     6841 acctttgttg ttattagcag cagaacaatg acgtggttag ttaattaaag ctgaaaataa
     6901 gctttttgcc tggattttta tggcggctta cgccgccgtt gaaactttaa tcatcaaccg
     6961 ccacgcggat caccactttg ccgaagtttt tacccttcag caggccgata aacgtctgtg
     7021 gcgcattctc taaaccgtca gtaatttctt cgcggtagtg gattttatcc tctttcaccc
     7081 attgccccat ctccttctga aactcttgga tgcggtgacc ataatcctga gcgataataa
     7141 atccttgcaa gcgaatacgt tttttcagca ccgtagccat cagcagaggt aaacgatccg
     7201 gaccgggtgg tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg
     7261 cagatgtatt aagtaaaggt agcactgcat caaatacctt accgcccacg ttttcataat
     7321 agatatcaat accttttggg cacgctttcg ccagttgttc ggcaaaatca tccgcgtgat
     7381 ggtcaagaca aacatcgaag cccagcacct cagtagcatg gcggcatttt tccacgccac
     7441 cggccacccc caccaccctg caacctttaa gtttgccgat ttgacccacc gtcgccccca
     7501 caggacctgt cgccgcagca acaaccaatg tttcgccctc tttgggctga ccgatatcca
     7561 gcagccccat ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat
     7621 tttgcggatg atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc
     7681 atccactgta gcccagcacc cagtcgccag gctggtaatc aggatgattc gactccacaa
     7741 cacggctcac cgtaccgccg accattacgc caccaatatc aaccggcggt gaataagatg
     7801 gctcatcgct catacgtcca cgcatatacg ggtccagtga caaataaact gtgcgcagta
     7861 acacctgtcc ttcacccggt gtggcgatat catcttcttc aagacggaaa ttctccggaa
     7921 ccggcgcgcc atgcggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc
     7981 ccataatgct ctcctgtgct tttggcatga atccatcaat agtagcaggc tcgcaggaat
     8041 ggttttgttt gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc
     8101 gttgcttcgg gctgttttcg atagtattga taacgtcatc atcattattt aatcgaggcc
     8161 aaacatgtcc atccgttttg cccgcaaagc cgactgtgct gccattgcgg aaatttataa
     8221 ccacgccgtg ttgtatacgg cggctatctg gaatgaccaa acggtggatg cagataaccg
     8281 cattgcctgg tttgaagcgc ggaccatagc aggttatcca gtgctggtga gcgaagaaga
     8341 tggcgtagtg acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca
     8401 taccgtggaa cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa
     8461 attgttaagc cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg
     8521 gatcgaatcg caaaatcagg cgtctttgca tctgcaccag tcgctgggat ttgtcgtcac
     8581 cgcgcaaatg ccgcaggtgg gcactaaatt tgggcgctgg ctggacctga catttatgca
     8641 gttgcaactc gacgaacgca gtgaaccgga cgcaatctga tgaatcagtc gctcaccctt
     8701 gcttttttga ttgccgccgg gattggtctg gtagtccaga acacgctgat ggtgcgcatc
     8761 acccagacct cctctaccat tctcatcgcc atgttgctga actctttggt aggaattgtg
     8821 ctctttgttt ctattttatg gtttaaacaa ggtatggcgg ggtttggcga actggtgtcc
     8881 aacgtgcgct ggtggacact tatacctggc ttgctgggat cgttttttgt cttcgccagt
     8941 atcagcggat atcagaatgt gggagcggcg acaactattg cggtactggt cgccagccag
     9001 ctgattggcg gtctggtgct ggatatcttc cgcagccatg gcgtaccact acgggcgttg
     9061 atcggcccaa tttgtggcgc aattttactt gtggttggtg cctggctggt ggccaggcgc
     9121 acattttaat cagaggatgg tgccaccttt ggttaactgt tcgtggcgcg cttccatatc
     9181 ttctttatgt ttgcgaccgt gatgggcgat cgctgtgcga agacgctgct gctgggtata
     9241 gcgttcttcc cgacttaact ccgcatcatc gctcagggcg attaataatt cgttcatatg
     9301 ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga
     9361 catactttct ccttagccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc
     9421 gtggtgccgt caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga
     9481 aactcatcgg ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat
     9541 cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa
     9601 gcatgtgttg atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa
     9661 tatgcgtcat tttttgacgt acatcaagtt ttttaaccgg tttgagaaaa aaacatcaaa
     9721 cagcaacttg cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta
     9781 ctatacacat aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta
     9841 gtactgataa atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc
     9901 tggtttattt gatgtagtga ttttgcacgc agcacgtact gtcagagaaa tcagtgctct
     9961 gacagtacgc aagatcatct catactctta cgatctgcaa tattatcctt aatgtttaac
    10021 catgacgtgg cggacgacgg tgtaatcctc cagcccataa agtgacatat ccttgccgta
    10081 accagaaagt ttctgcccac cgtgcggcat ttcacttacc agcatgaaat gggtattgac
    10141 ccaggtacaa ccatattgca gccgtgcgct gacgcgatgc gccctgccca catctttcgt
    10201 ccataccgaa gatgcaagtc cgtactggct gtcattcgcc cagtttacca cctgttcttc
    10261 gttgtcgaag agtgtaacac tcactactgg accaaatacc tctttttgca cgatggcatc
    10321 gtcctgtaat gcgccagcca gcagcgtcgg cgcatagtaa tagccattac ccttgcgctt
    10381 ttcaccgcca gtgatcactt tgatgtgccc tgtcgctttc gcctcttcta ctgccttgct
    10441 gacgcgttcg agatgcgcca gcgagcttaa aggtccaagc tccgtagact cgtcatccgg
    10501 cgcaccagat tttaacgttg ccaccgcagc acccagtttt tccaccagcg tatcgtaaat
    10561 gcctttttgc gcgtagatcc gacaagccgc agtacaatcc tgtccggcat tgtaatagcc
    10621 aaatgtacgt acaccttcga ccactgcctc aatatccgca tcatcaaaaa caatcactgg
    10681 cgctttgcca ccaagttcca tatgagtacg ctttatggac ggcgcggtat ggctgatgat
    10741 gtgttcccca gtggcgatag agcccgtcag cgacaccatc cgcactttgg gatgaccggt
    10801 cagcggatcg cccaccgttt tgcctctgcc aaacagtaca ttaatcacgc cagccgggaa
    10861 gatatctttc gccaactctg ccaacttcaa cgctgtcagc ggggtaattt ctgatggttt
    10921 aagcactacg cagttccctg ccgccagcgc cggagccagt ttccacgcag ccatcatcag
    10981 aggataattc cacggtgcga tagaagccac gacccccaac ggatcgcgac ggatcatcga
    11041 agtatgacct tcaagatatt cacctgccgc cagaccattc agacagcgcg ccgcaccagc
    11101 gaaaaagcga aaaacatcga caatcgccgg gatttcatca ttgaacgcac tatgcagcgg
    11161 tttgccacaa ttacgggact ccagttcggc aaaaacctga ccattttctt cgataacatc
    11221 agccagtttc agcagacatt ccgcacgcac tttcggcgtg gtttgccccc attcggcaaa
    11281 tgctgcatct gccgcgcgaa cagcagcatc gacctgctct gcggatgcct cggcaatctc
    11341 cagtaaaacg tcccccgttg ccggattata gacaggctgt ttttcccctt cgccgctaac
    11401 cagttctccg ttaatcagta acttatgttg catagcatat tcctgtatca gtggttattg
    11461 accattgtcg ccttcgcggg ttagccacca ggcccccagg atcggcaagg ttgttaccaa
    11521 cataaccagc agtgccacca cgttagttac cggtacatca cgcggtcgcc caagctgatt
    11581 gagcaaccac aacggtaacg ttcgttcatg acctgccgta aaggtcgtaa cgatgatttc
    11641 atcgaacgac aaggcaaacg ccagcattcc tcctgccagt aacgccgaac tgagattcgg
    11701 caacactacg tagcggaagg tttgccagcc attagcccca agatccattg acgcctcaac
    11761 cagactccac gaggtgcggc gaaaacgggc gatgacattg ttaaagacca ccactacaca
    11821 aaaagtcgca tgaccgacca cgatggtgaa aaatcccggc tccagattga tggttttaaa
    11881 ggcggttaat aacgccagac cagtgacaat gcccggcagc gcaatgggca gcagcagtaa
    11941 cagcgaaatg gcgtttttgc cgaaaaagtc tcgtcgccac agcgcggcag cggccagcgt
    12001 ccctaacacc agcgcgatta atgtcgccag cgccgccact ttaagtgaca gtgtcacggc
    12061 atcaagaata tcactacgct gtgctgccac gctaaaccag cgcagcgtca ggccttgcgg
    12121 tggaaaacta aacgccgcat cttcagtgtt aaacgcataa acggcgataa tcagaatggg
    12181 aaaatgtaag aaaaccacgc cgccccaggc cgccagtttg aggaaaaacg gtgcgcgttc
    12241 agagtgcatc gaacgctccc agacgtttca cgaacgctag gtaaagtgcg atgagtacaa
    12301 tcggcactag ggtgaatgcc gccgccatcg gcatattgcc aatcgccccc tgctgggaat
    12361 aaaccatatt gccgataaaa tatcctggag ggccaaccag ctgcgggaca ataaaatcgc
    12421 ccagcgtgag tgagaaggta aagatagagc cagcggcaat acccgggatt gccagcggca
    12481 gcaccacata gcgaaaggtt tgtcgtggac gtgcgccgag atcagctgac gcctgcaaca
    12541 atgacggcgg caaacgctca agcgccgcct gcaccgggag gatcatgaac ggcaaccaga
    12601 tatagagaaa caccagaaag cgccccagcc cggaagttga cagcgtatta cctcccaccg
    12661 ccggtagcgt taggaacgca gtcagcagtg gttccagccc cagatgttgt aaaaaccact
    12721 gcgccacgcc atcttttgcc agcaataacg tccaggcata ggctttcaca atgtagctcg
    12781 cccacatcgg caacattacc gcgatataaa aaaacgcttt cattttcccg ctggtatagc
    12841 gcgccatata ccacgccatt ggaaaagcca gaatggcgct ggcgatagtg actgccacag
    12901 ccataatcag cgtgcggaga atgatgtcgt aattcgccgg attaaacagc gcacggatat
    12961 tcgccagcgt cagttccggc gttaccgaca tggtgaaatc gtcaaaggta taaaatccct
    13021 gccataacag tgtcagcagc gagccgaaat agacaatgcc aaaccacatt agcgggccaa
    13081 gcagcagtaa aaacagcccc agccccggat taccccagaa gaaaccggac accttaccca
    13141 gacctggacg tgaaggtgat tgcaatacgt tcatcgccat tcacctctcc tcaaccagcg
    13201 gtaccatcac atcacgcgac caggaaacca tcacctgttg tccgggcgtg agcgtggcgg
    13261 gcagttcttc gcctgtcata ttggcctgac tcaccagcag tttttcaccg ccgttcagtc
    13321 tcagttcaaa acgggtcgcc gcgccctgat actgcaccgc ctggatcgtg ccattagcct
    13381 gcacttcgcc ggggatgttg aagcggatat gttccggtcg cagggcgaag cttcccgtca
    13441 tgccacaaag tttgtctgcc atcagtgcat caaaaacatt cgatgtcccg acgaacccgg
    13501 caacaaacgg cgtgcgcggg cgcatataga gatcacgagg ggaatcgacc tgctcaatgc
    13561 gtccattatt gaaaaccgcc acacgatcgg acatcgataa cgcttcgccc tgatcgtggg
    13621 taacgaagat aaaagtgata ccgagagact gttgcagttt tttcagttcc agctgcatct
    13681 gctcacgcaa tttgagatcc agtgcgccga gcggttcatc caacagcaat acgcgcggtt
    13741 cattcaccaa tgctctggcg atagcaaccc gctggcgctg accaccagaa agctgtgacg
    13801 gcttacgttg ctgaacaaaa ccaagcgcca ctttctccag cgcttcttgc gccatagcat
    13861 atcgctgttt tttatccacg cctttgacca tcagcccata ggccacgttg tcaagaatcg
    13921 acatatgcgg aaaaagtgcg tagtcctgga agacggtatt aacatcccgc tcccacggtg
    13981 gcagattgct ggcggattta ccaaagatag agatagcccc gccggaaagc tgttcgaagc
    14041 cagcaatcag gcgcaggcag gtggttttgc cggagccgga cggccccagc atagagaaga
    14101 actcaccatc ttttatcgca atactgacgc catctactgc gcgcacgtca ccgtacaacc
    14161 gtgagacgtt gtcaaactcc actgcgtacg tcataaaaca ccccagcgaa gttagcgacc
    14221 gcccataatg gcaatgtaat cctgcgtcca gcgactgtag ggaacaaact tgcccccttc
    14281 tgctataggc gttttccaga aggcgatttt atcgaaatag ttaaaaccgt tggtttcaca
    14341 acctttttca cctaataacg gactggcttt acacccttgc ggcactactg gtaacgagcc
    14401 aaaccaggcc gccacatcac cctgcacttt tggcgttaat gaccagctca tccatttgta
    14461 ggcgcaaacc ggatgtttcg cttcgctatg tagcatggtg gtgtcagccc aaccggtaac
    14521 gccctccttc gggaaaacgg tagcgacagg ctggccttcg gctttcaggg cgttggcctg
    14581 atagggccat gcactggaag caaccacacc ctcgtttttg aaatcgctca tttgcacggt
    14641 agtgtcatgc cagtagcgat ggatcaaact atgttgatcg cgcatcactt tcagtaccgc
    14701 ctgatactgt tcttcggtga gttgatacgg atcgcggatg cccaactgcg gctgagtggc
    14761 tttaacgaac aacgcagcgt ccgcaatgta gatagggcca tcataagcct gaacgcggcc
    14821 tttattgctc ttgccgtccg gcagattctg ctcaacaaaa accacttgcc agctatccgg
    14881 cggcgtcggg aaggttttag tgttgtacat cagcaggttc ggcccccatt ggtaaggtgt
    14941 gccgtaaact ttgccgccaa cgttaaacca gtcgccttta accacgcgcg gatcgagcgc
    15001 tttccagttg ggaatcaagg cggtattaat tggctgcacg cgtttaccca taatcaaacg
    15061 cagcgaggca tcgccggatg ccgtaaccag atcgtaaccc cctttggcca tcagactgac
    15121 catttcatcg gaagtcgcgg cggttttaac attcaccgcg cagcctgtct ctttttcgaa
    15181 ttgcgttacc cagtcgtatt gtttatcagt ttgtccgcgt tcgatgtatc ccggccaggc
    15241 gataatatcc agtcgccctt ccggtttatc taaattggta ggtggttcgg cggcgtgagc
    15301 ggtcattatt gtcatggtga gcgcacacag gctgctgcgg gcaaatgtct tgctcataaa
    15361 gtcttactcc tgtcgtaatt aattgttcag ccgccgtgcc gtaaaaatat ctccttgttt
    15421 aaccatagac ggcggctagc aggcacacct tcgcccctga gaaaatttaa tcaggttgtg
    15481 agctaccgcg cattatgccg tatgtaaggt tatcttctgg agtatagaca aggagttagg
    15541 aaatatgact gctattcgaa attcatatta cccaacctga gaggaattta attaaacgct
    15601 attacagccg ttcttgaata agtttgccta attgtttaac cgcctgttct tcacgttctc
    15661 cccactgcca cgcggtatta aaacggaaaa aacgtgacca gttttcaccg gtagaaaaca
    15721 ttttacccgg cgcaatactg atatgatgcg tcagtgctgc caggcttaat tcgcccgcat
    15781 ctaacggctc ggggagctcc agccagagaa aataaccgct gtcattatga tgaattttca
    15841 cttccgcagg cagataacgc agcagtgctt gccaggcacg ttgtttacgt tccgcaagct
    15901 ggcgacgcag gcgacgaaga tgggcgtcgt atcggcgcgt ggaaaggtaa tccaccagcg
    15961 caagctgcat cggtgagctg gtggaaagcg tactcatcaa ctgcaagcgt tgaattttac
    16021 gtgcatgttt tccggcggcg acccaaccaa tacgaaaacc aggcaccaga catttcgaaa
    16081 acgaagagca atgcaaaaca ccatcgtggc gatcccacgc tttcgcaggc agcggttttt
    16141 cccgtccaaa ataaagttcg ctgtaaacgt catcttcaat cagcgttacg ttgtactgat
    16201 tgagcaacgc taccagccgc gcttttttct gcggcgttaa ggtaaatccg agtggattct
    16261 ggctattagt catcagccag cacgctttca ccggatactc ctgcaacgcc agttccagcg
    16321 ccttgagatc aatcccttct ttaacgtccg tcgccaccga taacgccttc agccgtaacc
    16381 gctccagcgc ctgcaacgca ccgtagaaac aaggattctc tactatcacc caatcgcccg
    16441 gttcagtgac cgcttgtaaa ctgaggttta atgcctctaa cgccccggca gtaatgacaa
    16501 tttcatcggg tgaaatggtg atgccctgta aggcatagcg acgagctata gcctgacgca
    16561 gttcagcgtt tcctggcggt aagttttcaa tcacgctcat cgccgtggcg gttttgctta
    16621 cctgtgccag cgagcggttg agttgttgga gggggaaaag tcgcgggtcg ggaaaggccg
    16681 aggcaaacgg aactaccgac ggatcgcgac tggcctgcaa catatcaaaa atataggtgt
    16741 tgatatcgac tgcttcatct cgagtgactg gaatgactgg cgctttcggc atttttattg
    16801 cctgtgtcgc aacgtaataa cccgactgcg gtcgcgcgat aatatgtccc tgactttcga
    16861 gcaactgata ggcatggctg acagtcataa agctcatgcc agaaagcgcc acctggtcac
    16921 gcaacgaagg caaacgatcg ccaggttgcc agatacccga cgcaatctgc tcgcgtaatt
    16981 gttctgcaag ctgctggtat tttttcatta tcgaatcgta attatgtgca gatgattcgg
    17041 cagtctatat cagttgttaa aaatgacaac ttttcattaa ctgttatcaa accatcacca
    17101 gtgataactc tttgccaagc gccttagccg caagctggac ggcgtcgatt tttgtagcat
    17161 ggtgcaagtt aaataagcga gtaatctcct gtttaggttt gccaattcgt ctggctaact
    17221 cttgctgagt gatttctgac tgtaaaaaag catttaacag caatacttta gaggcgatac
    17281 ttaaaggcac ttcaataaag tgatcgttgt tagttaatgg cgaaggcaac gggattagct
    17341 cattatcttc aaaataaaaa tcaaatgcgg tgagtaaggc atcttttgct gcttccatcg
    17401 cttcagcaac agtttctccc tgcgtcaacg cttcagggat atctacaaaa gaaaccacat
    17461 aaccgccttc cggcgcagga gtaagagtga cgggataacg cataattttg actgtctctc
    17521 gcatgtcatt aaccttctta tggcgattaa tttaaaccga gttgcttcag gattgcttta
    17581 cgcaatggtt cttttatctc atcactgggg tgacgcggca tgacactgcg tcgcccttga
    17641 aacctgagtt tcaaatggtt gctgccattc gccacatcga cgccctgaga ttcgagccaa
    17701 cgtctgaact cgctttgttt cacatcgcct ccctgccgct gagtatatca acaaagtaaa
    17761 cattagtgtt tacctccgtg tgtaatttgc tcgaaaatag cactaaatcg ccgcaacggc
    17821 agaatgcaat gcaaaatcag gaaggaagat tccagaattt ctttgcgcta atttgcaaaa
    17881 ctgaataata acctcaagaa acgatcggca tcatagcgac aaaaggtaag aaaaaggtgg
    17941 caggatgttt acgaaggcgt tatcggttgt cttattaacg tgtgcactgt tctcaggaca
    18001 actcatggca gggcacaaag gacatgaatt tatgtgggta aagaatgtgg atcatcagct
    18061 gcgtcatgaa gcggacagcg atgaattgcg tgctgtggcg gaagagtcgg cggaaggttt
    18121 gcgcgagcat ttttactggc aaaaatcgcg caaaccagaa gcgggacatc gttgagtcat
    18181 ttgttgccgg atgcggcatg acgccttacc cggcaggcaa tatcatgcag atttgatgag
    18241 ttgtgagtaa ccagaaaact ggcgttttac ccttttcgct tcggtaaagt tttcagcagc
    18301 tcatccggac ttacggatgc cactacgctt cccggtaacg gcattttcag tatgtgattt
    18361 ttgattttgc caatgacgtg catctcgcat gggcggcaat caaactttag cgttaatact
    18421 tcatcgccgt taaccagttg cattggcgtc gccttccagc ttttgatatt gcctttcgcc
    18481 tgtttcgggc acagattgaa ggcaaagcgc agacaatgct tagtgatcat caccgggact
    18541 tcgcccttct cttcatgcgc ttcatacgcc gcatcaatca gttgcacacc atagcgatga
    18601 taaaattcac gcgctttctg gttgtaaacg ttcgcgagga aactcaaatg cgtttgcgga
    18661 taaaccggcg caggatcagc aaccggttta cggctgccgc gctggtaact ggcaagacgc
    18721 gcagcatcta gcatgtcggc ggcttcacgg cggaactggt ttaacagact gtttggtaca
    18781 aacagcgcac ccggcaaatt aatttgcaca tcgcgggcgt aatagatggt ttgccccagt
    18841 tttgccagac catcctttag attgttcatt gctttttcgg cgttattggc ttcgtcgaac
    18901 tgcccgtcca gcgtatgcgt gatgctgaca ccctcttcac tggtgagggt cagaatcagt
    18961 tgttcctgcc agccgcccag ttcaatgtct accgccaccc gacgttcgct ggaggttttt
    19021 gtcagtgcct gctgccagtt atgatcaaga ttacggttta gtgggtgatg cgggcgaatt
    19081 ttgtgcaact ctgctggcat ttcattgggc cagacgcggt actgattttc tccggttttc
    19141 tcgaccgtat tggcacgaaa gccgacgact tcacgtttaa tcatcacgtt caggccatcg
    19201 ccatttgcca gtggctcggt aacggcaaca tcgagatgat ctttcgccac tttcaatact
    19261 tcacccaccg gcaggccgat aaatttcggc gaatcgaacg cgccaatatc gcctttacgg
    19321 gcattcacaa aataatctgt gctaccacgg tggaaagtct tttccgtcga tggaacaaag
    19381 aaatgttcag tacgacctga tgaagcacgc gtcagatcgc cacgttcttc aataatggca
    19441 tccagcatct ggcgataatg ggcggtgata ttcttcacgt agctcatatc tttgtaacgc
    19501 ccttcaatct tgaaggagcg tacgccagca tcaatcagcg cgccgaggtt ggcagtctga
    19561 tcgttatctt tcatcgacag cagatgtttt tcataggaaa ccacccgccc ctgatcgtct
    19621 ttcaatgtgt atggcaaacg gcacgcctgc gagcaatcgc cacggttggc gctacgccct
    19681 gtttgcgcat gagaaatgta gcactgaccc gaataggcca cgcacagtgc gccatgaata
    19741 aagaattcaa tggtcgcgtc cgtggcctgg tgaatcgcgc ggatctgctc aagattcagc
    19801 tctcgcgcca gcacaatctg cgtgaagcca acatcagaga ggaacttcgc tttttctact
    19861 gtacgaatgt cgcactgcgt actggcgtgc agttcaatcg gcggaatatc aagttccaga
    19921 atccccatat cctgaacaat cagcgcgtcg acgccggtct ggtagaggtc ggtaatcagc
    19981 cgttgcgcag gttccagctc atcatcatgc aaaatggtgt taagcgtgac gaaaattttt
    20041 gcaccataac gatgggcaaa tggcaccagt tcggcaatat ctttcaggct attactggca
    20101 ttatgacggg caccaaaacc agggccgccg atataaacag catcggcacc gtgcaaaata
    20161 gcttcgcggg caatggcggc atcgcgtgcc gggcttaaca gttcaagtcg atgagaagat
    20221 acggtcacat tcttatccag tagctgaaaa atggcggcta ttttagccac aagcgcgacg
    20281 ttgcgaaata gttttcccgc ttaactgcgt gggtaatgga tgagggaatg aaaatgtacg
    20341 atttgctccc caccattacg atagacatgc gtaacgtctg cagcaaatcg agcgccttct
    20401 ccacaattaa ggctctgcca ttcaccatca acgcacagat caagttgccc atcaatgacg
    20461 acaacatgtt cgattactcc cttctcatgg ggcgtcgatt cacttattgc accaggtgcc
    20521 atctgaatcg agaagtgttc aaagcagagt tgtggatcat aaggaaacag cgaagtgatc
    20581 accatcacct gctgttgcgg atcataaact gaaggcgtag cggactgcgg cggagaaata
    20641 aatgtcgaaa atggcacatt caacccggtg gcaattttcc ataatgtcgc gaccgtcggg
    20701 ctggactcat tacgctcaat ctgccctaac atcgctttag aaacgcccgt cgcttctgcc
    20761 agtcgcgaaa gactccagcc gcgctgctga cgtagttgtt taagtgtggt ggataaaaag
    20821 cgagtgagat tttccatgcg actcctccgg caaaacggaa gtttatcact tgtgcgttat
    20881 aacggacaaa tgctacggta cctgtatgct ataacgcacg aggtgaccat gcgtctgttt
    20941 tctattcctc cacccacgct actggcgggg tttctggcgg tattaattgg ctacgccagt
    21001 tcagcggcaa taatctggca agcagcgatt gtcgccggag ccaccactgc acaaatctct
    21061 ggctggatga cggcgctggg gctggcaatg ggcgtcagta cgctggctct gacattatgg
    21121 tatcgcgtac ctgttctcac cgcatggtca acgcctggcg cggctttgtt ggtcaccgga
    21181 ttacagggac taacacttaa cgaagccatc ggcgttttta ttgtcaccaa cgcgttaata
    21241 gtcctctgcg gcataacggg actctttgct cgtctgatgc gcattattcc gcactcgctt
    21301 gcggcggcaa tgcttgccgg gattttatta cgctttggtt tacaggcgtt tgccagtctg
    21361 gatgatcaat ttacgttgtg tggcagtatg ttgctggtat ggctggcaac caaggccgtt
    21421 gcaccgcgct atgcggtaat tgccgcgatg attattgggg tcgtgatcgt tatcgcgcaa
    21481 ggtgacgttg tcacaactga tgttgtcttt aaacccgttc tccccactta tattagccct
    21541 gatttttcgt ttgctcacag cctgagcgtt gcactccccc tttttctggt gacgatggca
    21601 tcgcaaaacg caccgggtat cgcagcaatg aaagcagctg gatattcggc tcctgtttcg
    21661 ccattaattg tgtttactgg attgctggca ctgttttttt cccctttcgg cgtttattcc
    21721 gtcggtattg cggcaatcac cgcggctatt tgccaaagcc cggaagcgca tccggataaa
    21781 gatcaacgtt ggctggccgc tgccgttgcc ggcatttttt atttgatcgc aggtctgttt
    21841 ggtagtgcca ttaccgggat gatggctgcc ctgcccgtaa gttggatcca gatgctggca
    21901 ggtcttgcgc tgttgagtac cattggcggc agtttgtatc aggcgctgca taatgagcgt
    21961 gagcgcgacg cggcggtggt ggcatttctg gtaacggcaa gtggattgac gctggtcggg
    22021 attggttctg cgttctgggg attaattgcc ggaggcgttt gttacgtggt gttgaattta
    22081 atcgctaaca gaaaccgata ataaaaagcg ccatctaacg atggcgcaac tggtattact
    22141 ttttgttaac gtcaaacatg gtggcatcgg tcgccatgtc atcaataact tgtttaatat
    22201 tttcgaaagc cattggcgta ctttcgttat tcaggtcttt accttcgccc tgacgaacga
    22261 ctttgataac cggtttatta gtcgctgcat caatcagctc accttcaaaa tacaggcgag
    22321 tatccatggt acggtggccc gtcgccattt gcgtccccgc aaccactaat gcaacaggaa
    22381 ccacttcata gaattgcagc ccttctttgc tggtatctac accggtaatg gccccacgga
    22441 aaatcagact acgcggccca gcggtggtaa ccagtggttt acgctgcgca atcgcttctt
    22501 tcatttcggt gttggtataa ctcaaaattt tatccagaac tttctgccct acctgggtag
    22561 atggtttcgg taccggataa taagtgactg ggttccagac gatgctgtca tatttgcttt
    22621 gatcaaaact cgggtctacc caacgtaaaa caggtttacc tgttgctgag gttgtttctt
    22681 ttaaatcaga gtaattgttt aaaaaaccag aatatttatc tggctgggtg attttagagg
    22741 cacaaccaga cagagccaac aagccgcata aagctgcaac tttcgcaatt gatgtggtac
    22801 gcatgaatag tttcctgaga tgattatatt actttctaag ttatagcaaa aagcgccgct
    22861 tcgtgttgtg gcaaaaaaag tgttggtgaa aataaatttt ttagcc
//
LOCUS       DAZGHF010000076        22640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGHF010000076 DAZGHF010000000
VERSION     DAZGHF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.076"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2934
                     /locus_tag="ACHK3A_003036"
     CDS             <1..2934
                     /locus_tag="ACHK3A_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO8741088.1"
                     /translation="AAHGTDATSKITNVKDGNLTAGSTDAVNGSQLKTTNDAVAANTT
                     NIATNTTNITNLTDAVDSLGDDSLLWNATAGAFSAKHGTNGTDSKITNLLAGTVSSDS
                     TDAINGSQLYGLADSFTSYLGGGADISDAGVLTGPTYTIGGTDYNNVGDALAAINTSF
                     STSLGDALLWDATAKGGDGAFSAGRGKDNTASIITNVADGAISSTSSDAINGSQLYDT
                     SKYIADTLGGDAEVNADGTITAPTYAIAGGSYSNVGDALEAIDTTLDDALLWDATAND
                     GNGAFSAAHGKDKTASVITNVANGAISATSSDAINGSQLYTTNKYIADALGGDAEVNA
                     DGSITAPTYTIANAEYNNVGDALDALDDNALLWDATANDGAGAYNASHDGKASIITNV
                     ADGNIGEGSTDAINGSQLFNTNMMIQQNSEIINQLAGNTSETYIEDNGAGINYVRTND
                     NGLAFNDASASGIGATAVGYNSVAEGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIVK
                     GSRDTSVSEEGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQN
                     AIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLVLADAIN
                     GIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQI
                     TNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENGIGDIVS
                     TGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVANEENTISVGSST
                     NQRRITNVAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSVDYSNITLGGGNGGTTRI
                     SNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMT
                     GLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALG
                     AGIQW"
     gene            3346..5001
                     /gene="lldP"
                     /locus_tag="ACHK3A_003037"
     CDS             3346..5001
                     /gene="lldP"
                     /locus_tag="ACHK3A_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO8741089.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVIYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            5001..5777
                     /gene="lldR"
                     /locus_tag="ACHK3A_003038"
     CDS             5001..5777
                     /gene="lldR"
                     /locus_tag="ACHK3A_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO8741090.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5774..6964
                     /gene="lldD"
                     /locus_tag="ACHK3A_003039"
     CDS             5774..6964
                     /gene="lldD"
                     /locus_tag="ACHK3A_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO8741091.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIREFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            7037..7510
                     /gene="trmL"
                     /locus_tag="ACHK3A_003040"
     CDS             7037..7510
                     /gene="trmL"
                     /locus_tag="ACHK3A_003040"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO8741092.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPTEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7563..8384)
                     /gene="cysE"
                     /locus_tag="ACHK3A_003041"
     CDS             complement(7563..8384)
                     /gene="cysE"
                     /locus_tag="ACHK3A_003041"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO8741093.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8464..9483)
                     /gene="gpsA"
                     /locus_tag="ACHK3A_003042"
     CDS             complement(8464..9483)
                     /gene="gpsA"
                     /locus_tag="ACHK3A_003042"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO8741094.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9483..9950)
                     /gene="secB"
                     /locus_tag="ACHK3A_003043"
     CDS             complement(9483..9950)
                     /gene="secB"
                     /locus_tag="ACHK3A_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO8741095.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(10013..10264)
                     /gene="grxC"
                     /locus_tag="ACHK3A_003044"
     CDS             complement(10013..10264)
                     /gene="grxC"
                     /locus_tag="ACHK3A_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO8741096.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10406..10837)
                     /locus_tag="ACHK3A_003045"
     CDS             complement(10406..10837)
                     /locus_tag="ACHK3A_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO8741097.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVIVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            11082..12626
                     /gene="gpmM"
                     /locus_tag="ACHK3A_003046"
                     /gene_synonym="pgmI"
     CDS             11082..12626
                     /gene="gpmM"
                     /locus_tag="ACHK3A_003046"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO8741098.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12660..13919
                     /gene="envC"
                     /locus_tag="ACHK3A_003047"
     CDS             12660..13919
                     /gene="envC"
                     /locus_tag="ACHK3A_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO8741099.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13923..14882
                     /locus_tag="ACHK3A_003048"
     CDS             13923..14882
                     /locus_tag="ACHK3A_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO8741100.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(14869..15903)
                     /gene="waaH"
                     /locus_tag="ACHK3A_003049"
     CDS             complement(14869..15903)
                     /gene="waaH"
                     /locus_tag="ACHK3A_003049"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO8741101.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(16142..17167)
                     /gene="tdh"
                     /locus_tag="ACHK3A_003050"
     CDS             complement(16142..17167)
                     /gene="tdh"
                     /locus_tag="ACHK3A_003050"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO8741102.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARYVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17177..18373)
                     /gene="kbl"
                     /locus_tag="ACHK3A_003051"
     CDS             complement(17177..18373)
                     /gene="kbl"
                     /locus_tag="ACHK3A_003051"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO8741103.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18587..19519
                     /gene="rfaD"
                     /locus_tag="ACHK3A_003052"
     CDS             18587..19519
                     /gene="rfaD"
                     /locus_tag="ACHK3A_003052"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO8741104.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19529..20575
                     /gene="rfaF"
                     /locus_tag="ACHK3A_003053"
     CDS             19529..20575
                     /gene="rfaF"
                     /locus_tag="ACHK3A_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO8741105.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20579..21559
                     /gene="rfaC"
                     /locus_tag="ACHK3A_003054"
     CDS             20579..21559
                     /gene="rfaC"
                     /locus_tag="ACHK3A_003054"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO8741106.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKQFIEENAEKAAMI"
     gene            complement(21629..>22640)
                     /locus_tag="ACHK3A_003055"
     CDS             complement(21629..>22640)
                     /locus_tag="ACHK3A_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFO8741107.1"
                     /translation="HYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKDEMRTKIK
                     FGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASILIL
                     KSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALLLIVMDSYINKRINYKLYC
                     FIAIALLAGVFSFKDTLLTRMNDLNRDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIG
                     QSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDLIDTLSTRGIPGVALTILAFSAIF
                     IYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKAQSDQCLL
                     DK"
BASE COUNT         5624 a         5906 c         5837 g         5273 t
ORIGIN      
        1 gccgcgcacg gtactgatgc caccagcaaa atcactaacg tcaaagacgg taacctgacg
       61 gctggcagca ctgacgcggt taacggctct cagctcaaaa ccactaacga tgccgtggca
      121 gccaacacca ccaatatcgc cacgaacacc accaacatca ccaacctgac tgacgctgtt
      181 gacagtctcg gtgacgattc cctgctgtgg aacgctacgg cgggtgcatt cagtgccaaa
      241 cacggcacca acggtactga cagcaaaatc accaacttac tggcaggcac tgtatcctct
      301 gatagcactg acgccattaa tggctcacaa ctttatggtt tagctgattc atttacgtca
      361 taccttggcg gtggtgctga tatcagcgat gcgggtgtat taaccgggcc aacctacact
      421 attggtggta ctgactacaa taacgtcggt gatgctctgg cagccattaa cacgtcattt
      481 agcacatcac tcggtgacgc cctactctgg gatgcgaccg ccaagggcgg tgacggtgcg
      541 tttagcgccg gtcgcggaaa agataacacg gccagcatca ttactaatgt tgcagacggt
      601 gcaatttcct ctaccagcag cgacgctatt aacggctcac agctctatga caccagtaag
      661 tacattgccg atactctggg tggtgacgca gaagtcaatg ctgacggcac aatcaccgca
      721 ccgacttatg ctatcgctgg cggcagttac agcaacgtcg gtgacgcgct ggaagcaatc
      781 gataccacgc tcgatgacgc tctgttgtgg gatgccacag ccaatgatgg taatggtgca
      841 tttagcgccg ctcacggaaa agataaaaca gccagtgtaa tcactaacgt cgctaacggt
      901 gcaatctctg ccaccagcag cgatgccatt aacggttcac aactgtatac caccaacaag
      961 tacattgccg atgccctggg tggtgacgca gaagttaacg ctgatggttc tatcactgcg
     1021 ccgacttaca ccattgcaaa tgccgagtac aacaacgtcg gtgacgccct ggatgcgctc
     1081 gacgataacg ctctgctgtg ggatgcaacg gccaatgacg gcgcaggtgc ctacaacgcc
     1141 agccatgacg gcaaggccag catcatcaca aatgttgctg atggtaacat tggcgaaggc
     1201 agtaccgacg ccatcaacgg ttctcagctg tttaacacca atatgatgat ccagcagaac
     1261 agcgaaatca tcaatcagct tgccggtaac accagcgaaa cctacatcga agataacggt
     1321 gcgggtatta actatgtacg taccaacgac aacggcttag cgttcaacga tgccagcgct
     1381 tcaggtattg gcgctacggc tgtgggttat aactctgttg ccgaaggcga tagcagcgta
     1441 gccatcggtc agggcagcta cagcgacgtt gataccggta ttgccctggg tagcagctcc
     1501 gtttccagcc gtgtaatagt taaaggttct cgtgacacca gcgtgtcgga agaaggtgtt
     1561 gtgattggtt atgacaccac ggatggcgaa ctacttggtg cgctgtctat tggtgatgac
     1621 ggtaaatatc gtcaaatcat caacgtagcc gatggttccg aagcccatga cgccgttacg
     1681 gttcgccagt tgcaaaatgc cattggtgca gtcgctacca cgccgaccaa atacttccac
     1741 gccaactcaa cggctgaaga ctcactggct gtcggggaag actcgctggc gatgggcgcg
     1801 aaaactatcg tcaatggtaa tgcgggtatt ggtatcggcc tgaatactct ggttctggca
     1861 gatgccatca acggtattgc tatcggttct aacgcacgcg ccaaccatgc aaacagtatt
     1921 gcaatgggta atggttctca gaccactcgc ggcgctcaga ctggctacac cgcctacaac
     1981 atggatgcac cgcagaactc tgtgggtgaa ttctccgtag gtagcgaaga cggtcaacgt
     2041 cagatcacca acgtcgcagc ggggtcggcg gataccgatg cggttaacgt gggtcagttg
     2101 aaagtaacgg acgcgcaggt ttcccagaat acccagagca ttactaacct gaatactcag
     2161 gtaacgaatc tggatacccg cgtcaccaat atcgaaaacg gtattggcga tatcgtaagc
     2221 accggtagca ccaagtactt caagaccaac accgatggcg tagatgctaa cgcgcagggt
     2281 aaagacagtg ttgcgattgg ctctggctct attgctgccg ctgataacag cgttgcgctg
     2341 ggtactggct ctgtggcaaa cgaagaaaat acgatctccg taggttcttc cactaaccaa
     2401 cgccgtatca ccaacgttgc cgctggtaaa aatgctactg acgcggttaa cgttgcgcag
     2461 ttgaagtctt ccgaagcagg cggcgtgcgg tatgacacca aagctgacgg ttctgttgat
     2521 tacagcaaca tcactctcgg cggtggtaat ggcggaacga ctcgcatcag caacgtttcc
     2581 gctggcgtta acaacaacga cgcggtgaac tacgcgcagt tgaagcaaag cgtgcaggaa
     2641 acgaagcaat acaccgatca gcgcatggtt gagatggata acaaactgtc caaaaccgaa
     2701 agcaagttga gcggtggtat cgcttctgca atggcgatga ccggtctgcc gcaggcttac
     2761 acgccaggtg ccagcatggc ctctattggt ggcggtactt acaacggtga atcggctgtt
     2821 gctttaggtg tgtcgatggt gagcgccaat ggtcgttggg tctacaaatt acaaggtagt
     2881 accaatagcc agggtgaata ctccgccgcg ctcggtgccg gtattcagtg gtaatcatcc
     2941 attaacgaat gggtaaacgc cacattgcct gatgcgctac gcttatcagg cctacggggt
     3001 gattcagcac taccctgtag gccggataag gcgttcacgc cgcatccggc aagataaggc
     3061 gcaccagatc aacaatctga gggcaattct ctgatgggaa ttgccctttt cttcattacc
     3121 tctctcccag aattggccct accaattctt cattgatctg acctctggtt cacaattccc
     3181 caattaaaac tcacatcacc gttgccaata cataacatct agttaactat tcattgtcat
     3241 tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag
     3301 caacagactc attacacggc gtgtgtggac tgcaggagac ctgcaatgaa tctctggcaa
     3361 caaaactacg atcccgccgg gaatatctgg ctttccagcc tgatagcatc gcttcccatc
     3421 ctgtttttct tttttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg
     3481 acggtggcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg
     3541 ctggcctcgg tgatttatgg cttcttctac ggtttgtggc ccatcgcgtg gatcattatt
     3601 gcggcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat catccgctcg
     3661 tctattcttt cgataacccc tgaccagcgc ctgcaaatgc tgatcgtcgg tttctgtttc
     3721 ggcgctttcc ttgaaggagc cgcaggcttt ggcgctccgg tagcaattac cgccgcattg
     3781 ctggtcggtc tgggctttaa accactctac gccgccggac tgtgcctgat tgttaacacc
     3841 gcgccagtgg catttggtgc gatgggtatt ccgattctgg tcgccgggca ggtaacaggc
     3901 atcgacagct ttgagattgg tcagatggtg gggcgtcagt tgccgtttat gaccattatc
     3961 gtgctgttct ggatcatggc gattatggac ggttggcgcg gaatcaaaga aacgtggcct
     4021 gcggtcgtgg tggcaggtgg ttcgtttgcc attgctcaat acctcagctc taacttcatt
     4081 gggccggaac tgccagacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc
     4141 ctcaaacgct ggcaaccagt gcgcgtattc cgtttcggtg acttgggggc gtcacaggtt
     4201 gatatgacgc tggctcacac cggttacacc gcaggtcagg tgcttcgcgc ctggacaccg
     4261 ttcctgttcc tgaccgccac cgtaacgctg tggagtatcc cgccgtttaa agccctgttc
     4321 gcttcaggcg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa
     4381 ctggttgccc gtatgccgcc agtggtcagc gaagcgacag cgtatgccgc cgtgtttaag
     4441 tttgactggt tctctgccac cggtaccgcc attctttttg ctgccctgct ctcaattgtc
     4501 tggctgaaga tgaaaccatc tgacgctatc agcaccttcg gcagcacgct gaaagaactg
     4561 gctctgccta tctactccat cggtatggtg ctggcgttcg cctttatctc gaactattcc
     4621 ggactatcat caacgctggc gctggcactg gcgcacaccg gccatgcatt caccttcttc
     4681 tcgccgttcc tcggctggct tggtgtcttc ctgaccggat cggatacctc atctaacgcc
     4741 ctgttcgccg ccctgcaagc cactgcagca caacaaattg gcgtttctga cctgttgttg
     4801 gttgccgcca acaccaccgg tggtgtcacc ggtaagatga tctctccgca atctatcgct
     4861 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
     4921 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc
     4981 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
     5041 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
     5101 aacgtcaact ggcgatgcaa ctcggcgtgt cgagaaattc actgcgcgag gcgctggcga
     5161 aactggtcag tgaaggtgtg ctgctcagtc gacgtggcgg cgggacgttt attcgctggc
     5221 gtcatgatac atggtcggag caaaacatcg tccagccgct gaaaacgctg atggccgatg
     5281 atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcgt
     5341 ggcatgcggc aatgcgcgcc acaccaggcg aaaaagagaa aattcagctt tgctttgaag
     5401 caacgctaag tgaagacccg gatctcgcct cacaagcgga cgttcgtttt catctggcga
     5461 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
     5521 tgcaatcctc tgtgaagcat agccgccagc ggatgtatct ggtacccccg gttttttcac
     5581 agctgaccga acaacatcag gcagtcattg acgccatttt tgccggtgat gctgacgggg
     5641 cgcgtaaagc aatgatggcg caccttagct ttgttcacac caccatgaaa cgattcgatg
     5701 aagatcaagc tcgccatgca cgcattaccc gcctgcctgg tgaccataat gagcattcga
     5761 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cacaacgcat
     5821 tctgccgccg ttcctgttcc actatatgga tgggggtgca tattctgaat acacgctgcg
     5881 ccgcaacgtg gaagatttgt cagaagtggc gctgcgccag cgtattctga aaaacatgtc
     5941 tgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tggcgctagg
     6001 tccggtaggt ttgtgtggca tgtatgcgcg acgcggcgaa gttcaggctg ccaaagccgc
     6061 ggatgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
     6121 ggctccagcc attaaacgtc cgatgtggtt ccagctttat gtgctgcgcg atcgcggctt
     6181 tatgcgtaac gcactggagc gagcaaaagc ggcaggctgc tcgacactgg ttttcaccgt
     6241 ggatatgccg acaccgggcg cgcgttaccg tgatgcacac tcgggtatga gcggcccaaa
     6301 tgcggcaatg cgccgttact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
     6361 cctgaacggt cgtccacatg atttaggtaa tatctcggct tacctcggca aaccgaccgg
     6421 actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga
     6481 ccttgagtgg atccgcgaat tctgggatgg tccgatggtg atcaaaggga tcctcgatcc
     6541 ggaagatgcg cgcgatgcag tacgttttgg tgctgatggg attgtggttt ctaaccacgg
     6601 tggccgccag ctggacggtg tactctcttc cgctcgtgca ttgcccgcta ttgcggatgc
     6661 ggtgaaaggt gatattgcca ttctggcaga tagcggaata cgtaacgggc ttgatgtcgt
     6721 gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct
     6781 ggcaacagcg ggccaggcgg gcgtggcaaa tctgctgaat ctgatcgaaa aagagatgaa
     6841 agtggcaatg acgctgactg gcgcgaaatc gattagcgaa attacgcaag attcgctggt
     6901 gcaggggctg ggtaaagagt tgccggcggc actggctccg atggcgaaag ggaatgcggc
     6961 atagttgggg gtgaggggaa aaggccgtac caatcaccaa ttttctgcta tcctgccccc
     7021 gcactaaggg ggcagtatgc taaacatcgt actttacgaa ccagaaattc cgccaaatac
     7081 tggcaacatc atccgtcttt gcgctaatac cggctttcgt ctgcatatca tcgaaccaat
     7141 gggatttgcc tgggacgata agcgcctgcg ccgcgcgggg ctggactatc acgagtttac
     7201 cgccgttacg cgtcatcatg actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct
     7261 gtttgccctc accacgaaag gtacgcctgc tcacagcgcc gtaagctatc aggatggcga
     7321 ttatctgatg ttcggcccgg aaacacgcgg cctgccagcg agcattcttg atgccctgcc
     7381 cactgaacaa aaaattcgta ttccaatggt gccggacagc cgcagcatga acctgtctaa
     7441 tgcggtgtcg gtcgtggtgt atgaagcctg gcggcagttg gggtatccgg gagcggtgtt
     7501 gagagattag ttactgtagg ccggatagat gattacatcg catccggcac gatcacagga
     7561 catcagatcc catcaccata ctcaaacgta tggttaatgc cgttgaaatg ctgatccata
     7621 tccattgacg gtttatcgct atccggcttg ccgacaatgc gtgccggaac gccagcggca
     7681 gtggtgtgcg gcggcaccgg ttgcagcacc acggaacctg cgccaatctt cgcgccgcgc
     7741 ccaacttcaa tattaccgag aattttcgcg cccgcgccaa tcatcacgcc ttcacgaatt
     7801 ttcgggtgac ggtcaccacc agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc
     7861 gatacgtcgt tttcaatcac cgccgtttca cccacaacga tgcccgtcgc gtggtcgagc
     7921 atgataccgc gaccaatttt tgctgccggg tgaatatcaa cctggaacgt tacagagacc
     7981 tggttttgta gaaaaatagc cagcgcgcga cggccctgat tccacaacca gtgaccgatg
     8041 cgatacgcct gcaaagcatg aaaacccttc aggtatagca acggggttga gtatttatcc
     8101 accgccgggt cacgggtacg caccgcctga atatcacagg ccgcagaggc gatcatttcc
     8161 gggtcggcgg cgtaggcttc ttccaccact tcacggatag caatagccgg cataatgggc
     8221 gatgacagct tgttcgccag catgtagctc agtgcactgc caaggttttc gtgcttgagt
     8281 agcgtcgcgt ggtaaaaact ggccagcatt ggctcacagt ctgccagcgt tctggcttca
     8341 gctttaatat tgttccagac aatttccagt tcttcacacg acattgctta ctccacacga
     8401 taagataatg accggcccgt tctgcgcggg ccgggtcata gtggtaacaa aggttccctg
     8461 ggtttagtgg ctgctgcgct cgtccttgcg tgcacgacct agtaaagtca atgctgcctc
     8521 gcgcgcgttt tttccgcaat ataatacttg ataaatttcc tcggttattg gcatttcaac
     8581 gccgaagcga tgcgccagtt cgcggacttc tttcgtattg cggtagcctt ccaccacttg
     8641 accaattttc tcctgcgcgc tttgtacatc catgccctga ccgagcatca tgccaaaacg
     8701 gcggttacgc gactggttgt cggtacaggt cagcaccaga tcgcccagcc ccgccatgcc
     8761 cataaaggtg gcaggatcgg cacccagcgc cgcgccaaga cgcgacatct cagccagccc
     8821 acgggtgatc agcgccgtac gcgcattcgc gccaaaaccg ataccgtcgg acatccccgc
     8881 gccaatggca atgacgtttt tcaccgcgcc gccaagctgc acaccgatga aatccgggtt
     8941 gctgtaaacg cggaaacttt tgccacagtg cagcaattgc tggaggtcat cggcaaaggt
     9001 ctggtccgtc gaggccagcg aaatggctgt cggtaaacct gccgccagtt ctttcgcaaa
     9061 cgttgggcca gagataaccg ccagcggaat ttgatcgcct aaggcctcac gcgctacgtc
     9121 ctgtaacaga cgtccggttt ctgcttccag ccctttggtc gcccacacca gacgcgcatc
     9181 aggacgcatc agcggtttaa tctggcgcag cacttcacca aagacatggc tgggtacgac
     9241 gacgagaata ttacggctgg ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt
     9301 atcgggaaaa ggcacatcgg ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc
     9361 gatatgttca gggtcatggc cccagaggac aacctcgtgg ccatttcttg ccagggtaat
     9421 ggcaagagcg gtgccgtacg agccggcacc gatcacagtc attgaagcat tacgttggtt
     9481 catcaggcat cctgatgttc ttcagtacct tcgccagcct gctgctgcaa atagttcatg
     9541 aacagcgcat cgaagttaac cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc
     9601 atgctggtga tgcactcacg agcatacggg aacagaatgt tcgggcagta tgcacccagg
     9661 caatgtgcca tctgggtgcc ttcgataccc gcgatggaga aaataccgcc ctgctgaact
     9721 tcacacagga acgcggtttc ttcgcccagg gaggccgtta cggtaacacg cagtaccact
     9781 tcgtaaacgt catctgccag ttgagaagat gccgtatcca gatcaagttt aacttctggc
     9841 tgccaatctt tctggaaaac gtgcggcgcg ttcggcgctt cgaaagaaat atccttggta
     9901 taaatacgtt ggatctggaa agtcatttcg gtgttgtttt gttctgacat gtgtagaaaa
     9961 cccttaagtg ttgtccttaa atactgcgca atgtaatgcc aacgttcaca cgttatttca
    10021 gcaggggatc cagtccacca cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt
    10081 gctgtgcgtc aataaaaatc tggggaaccg tggtgcgacc gctgcgtttg atcatctctt
    10141 cacgcttagc ggcgttgcca tcgatcggca actcctgaaa actcacgccc ttgctgctca
    10201 gcagtgcttt cgcacgatgg caatacgggc aggtttcttt ggtatagatt tcaacattgg
    10261 ccataactta gctcctgttt ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat
    10321 ggagtgtccc cagaagctta taacaataag tcgctggggg cctaattgca gctaacggcc
    10381 tgcatcatga aagacgacag gtaaattatt tgccgcgcac cagaggtaag ttttcgccag
    10441 cccagccagc aacgccctct ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg
    10501 cgtttgcagg ctcctggcac tgcatgccag aaccgtctac cacgataacc ggtttgtctt
    10561 tgtgcttctc aagctcacca acattgttgg ctttgatttc gctcggcaac aggttgatag
    10621 aacctgcgat atggccttta cggaagtcat cacgctgacg taaatccaca accacagcat
    10681 cttctttgtt gatcagacgc gtagcttcac cacgagtaat caccttcact ttcgaggtca
    10741 ggctcttaaa cgtagtcaca ataaccgcca ccagtaacgc gatccaggcg atactcagta
    10801 tgggatgacg gccaacaaat tgcataattt cttgcatggg gggtaacaac tcccgacgta
    10861 gtgattaaaa aaaccaggaa aggagtatac ctgcgcggtg cagcaaatac agccagcgcg
    10921 ttaactggaa tgcaattttg cggggcgcga cgaaaaaaaa gcgcaaatct ggcatcggct
    10981 tgctgcgagc ggtcgtatat tttgatcttc agaggctatt ttatcgattc agctgtagta
    11041 aaattacgca aattttgact cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac
    11101 ctatggtact ggtgattctg gatggctatg gctatcgtga agaacagcag gataacgcca
    11161 tttttagtgc taaaaccccg gtaatggatg cactgtgggc caatcgtccg cataccctga
    11221 tcgacgcttc cggtctggaa gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag
    11281 gtcacgttaa cctgggtgcc ggccgcatcg tgtatcagga cctgactcgt ctggacgttg
    11341 aaatcaaaga tcgcgctttc ttcgctaacc cggtactgac tggcgcggta gataaagcga
    11401 aaaacgcagg caaagcagtt cacattatgg gtctgctctc cgcaggtggt gtacacagcc
    11461 acgaagatca catcatggcg atggtagaac tggcagctga acgcggcgca gaaaaaatct
    11521 acctgcacgc attccttgac ggtcgcgaca ctccgccgcg cagtgctgaa tcctcgctga
    11581 aaaaattcga agagaaattt gccgcactgg gcaaaggccg cgtagcgtcc atcattggtc
    11641 gttactacgc catggaccgc gataaccgtt gggatcgcgt tgaaaaagct tatgacctgc
    11701 tgactctggc gcagggcgag ttccaggccg ataccgccgt tgctggtttg caggctgctt
    11761 atgctcgcga cgaaaacgac gaattcgtga aagcgaccgt tattcgtgca gaaggtcagc
    11821 cagacgcggc aatggaagac ggcgatgcgc tgattttcat gaacttccgt gctgaccgtg
    11881 cacgtgaaat cactcgtgca ttcgtaaatg ccgattttga cggcttcgcg cgtaagaaag
    11941 tggttaacgt cgatttcgtg atgctgaccg aatacgctgc cgacatcaaa accgcagtgg
    12001 cttacccacc cgcttccctg gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa
    12061 ctcagctgcg tatttccgaa accgaaaaat atgcccacgt tactttcttc ttcaacggtg
    12121 gcgtagaaga gtcgttcaaa ggcgaagatc gcattctgat caactcaccg aaagtggcta
    12181 cctacgatct gcaaccggaa atgagctccg cagagctgac cgaaaaactg gttgcggcaa
    12241 tcaagagcgg caaatacgac accattatct gtaactatcc gaacggcgac atggtaggtc
    12301 acaccggagt gatggaagcg gcggttaaag cggttgaagc gctggatcac tgcgtggaag
    12361 aagtcgcgaa agcggttgag tccgtgggtg gacaactgct gatcaccgct gaccacggta
    12421 acgctgagca gatgcgcgat ccggcaacgg gtcaggcaca cacggcacat accaacctgc
    12481 cagttccgct gatttacgtt ggcgataaga acgtgaaagc ggttgaaggc ggcaaacttt
    12541 ctgatatcgc gccgaccatg ttgtcgctga tgggtatgga aatcccgcaa gagatgactg
    12601 gtaagccgct gttcatcgtg gaataatccc tccccatgag gggaaaggcg attaatacca
    12661 tgacacgggc cgtgaaaccg cgcaggtttg caatcaggcc catcatctac gccagcgttc
    12721 ttagcgctgg cgtattgttg tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca
    12781 aatctattca ggccgatatc gccgcgaaag agcgcgcggt acgccaaaag caacaacaac
    12841 gcgcaagcct gctcgcacaa ttgaaaaagc aggaagaagc gatctcggaa gccacccgta
    12901 agctgcgtga aacgcaaaac acgctcaatc aactgaataa acagattgat gagatgaacg
    12961 cgtcgattgc caaactggag cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac
    13021 tagatgccgc attccgtcag ggcgagcata ccggtattca gctgattctc agcggtgaag
    13081 aaagccagcg tggacagcgt ttacaggctt atttcggcta tctcaaccag gcgcgacaag
    13141 aaaccattgc tcagttgaaa caaacgcgtg aagaagtcgc tatgcaacgc gccgagctgg
    13201 aagagaaaca gagcgagcaa caaacgctgt tgtatgagca gcgcgcccaa caggcgaagc
    13261 tgacccaggc gctgaacgag cgtaaaaaga cgctggcagg gctggagtct tccatccagc
    13321 aaggtcagca acagttgagc gagctgcgcg ccaacgaatc ccgtctgcgt aacagcattg
    13381 cccgtgcgga agccgcggcg aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg
    13441 ttcgcgaccg ccagaaagaa gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg
    13501 aaaaatcgct gatgtcccga actggtggcc tgggtgcgcc gcgtggtcag gcattctggc
    13561 cagttcgcgg gccgacgctg catcgctatg gcgagcagct acagggtgaa ttacgctgga
    13621 aaggaatggt tatcggtgcc tctgaaggta ctgaagttaa agccattgcc gacggtcggg
    13681 tgattctggc tgactggctg caaggctacg gtctggtggt ggtggttgag catggtaaag
    13741 gcgacatgag tctttacggc tataatcaga gcgcactggt gagcgttggt tcgcaggttc
    13801 gcgcgggcca gccgattgca ctggtgggca gcagtggcgg tcagggtcgg ccttcactct
    13861 atttcgaaat tcgccgccag ggtcaggcgg tcaatccaca gccgtggttg ggaagataag
    13921 ttttgtttcc atttcgtcgt aacgttcttg catttgccgc tctgttggcg ctctcctccc
    13981 ccgtaattgc tggcaagctt gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg
    14041 aaaaccaggt gctggcgatg ccttccgcta tctccgtcgc tgtattaccc gattcaccgc
    14101 acgccagaga gatggcaacc aaagcgcata acagcggtca cgaagtgttg attcatctac
    14161 cgatggcacc actgagtaaa cagccgctgg agaaaaatac gctacgcccg gagatgagca
    14221 gcgacgaaat tgagcgcatt attcgtagtg cggtcaataa cgtgccctat gccgtgggga
    14281 tcaacaacca catgggcagc aagatgacct ctaacctgtt tggtatgcag aaagtgatgc
    14341 aggcgctgga gcgttacaat ctttacttcc tcgacagcgt aaccatcggt aatacccagg
    14401 cgatgcgcgc agcgcaaggc actggcgtga aggtgatcaa acggaaagtg ttcctcgacg
    14461 attcgcaaaa tgaagcggac atccgtgtgc agttcaatcg cgcaattgat ctggcgcgtc
    14521 gcaacggttc aaccattgcc attgggcatc ctcacccttc aacggtacgc gtgctacaac
    14581 agatggttta taacctgccg ccagacatta cgctggtgaa agccagcagc ttgctgaatg
    14641 aaccgcaggt tgatacgtct acaccgccga aaaacactgt gcccgacaca ccgcgtaatc
    14701 cgttccgtgg cgtgaagctc tgcaaaccga agaaaccgat agaacctgtt tatgctaatc
    14761 gcttctttga agtattaagc gaaagcatca gccagagcac gctgatcgtt tacttccaac
    14821 atcagtggca aggctggggc aaacagcctg aagcggcaaa acttaacgct agcgtaaatt
    14881 aaaggcgcta cgcgtaatgc gatggtgcga ccgcgttttg tcgcgccatt gccataagcg
    14941 gaaagaccac agtaacgcct gataacccac cttcacgctg cgcacattgg taatcaggcg
    15001 cttatacata ccggaagtaa atatctcggc aatcatccgt tggcgggtaa taatatccgg
    15061 ctctttgcgc accgcatggc aaacacgcaa tgcttcgtaa gttatttgct gatgaaattc
    15121 cggataaatc gtaattttgt cggcataatt tcgatttaat ttctctagca ggcgggtaat
    15181 cttaatatag tgacgttgat aattaaggtt tttattccct tgtctattca accgactcac
    15241 tgacgtatta tgcagataat atttatataa cgattgctcg gtatatcgcg cacgcagcgc
    15301 attaaacata aattctgttg tccagacaat atcctgatga tgtaatccgg caataaattt
    15361 aatgttattt ttaacaataa catcgcggcg ataaaccccc atccagacaa cgtgagtcca
    15421 acggcgggaa gaaagcccca tccgcaacca atccgggccg gttaatacgc cggttgagcg
    15481 aaggcgatcg gtggggatgg attgccaggt ttctcccgtt tcacgaaaac accagtcagc
    15541 gttgcactgc gccacgtcga ggtcgtcctc taacgccatg gtcatcagcg tttcgtacat
    15601 ggtgggatag acctcatcgt cagcatcgac aaaagcgaca tatttgcccg tcgccacctc
    15661 gatcccacga ttacgcgcta ccgatgcgcc agcattcgcc tgatgcaaca aacgaacgtg
    15721 cggatagttt tctgcgtaat gctttgctat ttcaacagaa ttatccgttg aaccatcgtt
    15781 aataataatg atttccagag cagtccaggt ttgcgtaatt aaagattcca tacacgtgcg
    15841 gaaatcatcg cccgcattat ataacgggat aataacacta agtttattgg tgctgttcat
    15901 cataaattac cgacgacctc tgaagttatc ctgacgtttt acaggctttg tattaagaaa
    15961 ctattaagcc tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat
    16021 aatgagaaca gctatataag agatcagcgc aattacgaac tctttattac actgacagga
    16081 ttttacatat ttaacggatt atcctcagga aaaagggctg gaataccagc ccttgtttgt
    16141 gttaatccca gctcaggata actttcccgg actggcccga acgcatggcg tcaaagccct
    16201 tctggaaatc atcgatagag aaacgatgag taatgatcgg cgaaagatcg aggccagact
    16261 gaatcagcgc cgccatcttg taccaggttt caaacatctc acgaccgtaa atacctttaa
    16321 tgaacaagcc tttaaagatc actttggtcc agtctatcga catgtcagaa ggtggaatac
    16381 ccagcatcgc aatacgaccg ccgtggttca tggtgtcaag catggtacga aacgctggcg
    16441 gcgcaccgga catttccaga ccgacatcaa agccttcggt catgcccagt tctgccatca
    16501 catcattgag gttctcttta gcgacgttaa ccgcacgggt gatacccatt ttacgcgcca
    16561 actcaaggcg gtattcgtta acatcagtaa tcaccacgta acgtgcacca acgtgtttcg
    16621 ccaccgccgc cgccataata ccaatcgggc ctgcaccaga gaccagcaca tcctcgccca
    16681 ccagatcgaa cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca
    16741 gatcatcgga gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatact
    16801 cggcgaagca gcccggacgg ttaacgccga cgccgatcgt gttgcggcac agatgggtac
    16861 gaccgccacg acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat
    16921 cgccaatctt gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat
    16981 gacccacgac catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt
    17041 gaacgtcagt cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga
    17101 gttccggtac aggaacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg
    17161 ctttcatctc acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt
    17221 ctactgcacg cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg
    17281 cctgaccttt cggaacgacc ggatagaaga aaccggtgac gtaaatcccc tctttttgca
    17341 gctcacgggc aaatttctgc gccactaccg catcgccaag catgaccgga ataatggcgt
    17401 gatcggctcc cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt
    17461 tcgcccacag acggtcacgc agctcgctac ccgcttcaac catctccagc actttgatgg
    17521 aagcggcaac aatggccggt gccagcgagt tggagaacag gtacggacga gaacgctggc
    17581 gcagccactc aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg
    17641 ctttaccaag cgtaccggtg ataatatcga cccgccccat cacatcgcag tattcatggg
    17701 aaccacgacc gttttcaccg acaaaaccta ccgcgtgtga gtcatctacc atcaccaggg
    17761 catcatattt atctgccaga tcgcaaacgc ctttcaggtt ggcaatcacg ccgtccatcg
    17821 agaacacacc atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca
    17881 gacgtgcttc cagctcctgc atatcgttgt tggcataacg atagcgttta gctttgcaca
    17941 gacgcacacc atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg
    18001 cgcccaacag cgtttcgaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg
    18061 catcttccat ccccaggaag gccgccagtt tttgttcaag ctctttatgg ctgtcctgag
    18121 tgccgcaaat aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg
    18181 ccgccgcaat cagatccgga tgattcgcca gtccgagata gttattggca caaaagttaa
    18241 tgacgtggct tccatcagcc acagtgatat ctgcttgctg cgcagacgta ataatgcgct
    18301 cttctttaaa caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa
    18361 attctccacg cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt
    18421 ataggttgcg acgagatgac gcgttaacac ttctgcaaaa ttcaggataa ataacgtcca
    18481 gccccctcac ttttggtgaa tggctgaagc caacgtcatg tagtgatatg atagaagtat
    18541 tcgtgtctga gattgtctct gactccataa ttcgaaggtt acagttatga tcatcgttac
    18601 cggcggcgcg ggctttatcg gaagcaacat cgttaaagcc ctgaatgata aaggcatcac
    18661 cgatattctg gtggtggaca acctgaaaga cggcaccaag tttgtgaacc tggtggatct
    18721 ggatatcgcg gactatatgg ataaggaaga cttcctgatc cagattatgg ctggcgaaga
    18781 gttcggcgat gtcgaagcga ttttccacga aggcgcgtgc tcttccacca ccgagtggga
    18841 cggcaagtat atgatggata acaactatca atactccaaa gagctgctgc actactgtct
    18901 ggagcgcgaa atcccgttcc tgtatgcctc ttccgcagcc acttacggtg gacgcacctc
    18961 cgactttatt gaatcccgcg aatatgaaaa accgttgaat gtctacggtt actcaaaatt
    19021 cctgtttgat gaatatgttc gccaaatcct gcctgaagcg aactcacaga ttgttggctt
    19081 ccgttatttc aacgtttatg gaccgcgtga aggccataaa ggcagcatgg cgagcgtcgc
    19141 tttccatctc aacacccagc ttaataacgg tgaatcaccg aagctgttcg aaggtagcga
    19201 gaacttcaaa cgtgacttcg tctatgtggg cgatgtggca gatgtaaacc tgtggttcct
    19261 ggaaaatggc gtttccggca tcttcaacct cggtactggt cgtgcggaat ccttccaggc
    19321 agtagcagat gctacgcttg cttatcacaa gaaaggccaa atcgaataca ttccgttccc
    19381 ggataaactg aaaggccgct accaggcgtt cacgcaggca gatctgacaa atctgcgcgc
    19441 ggcgggttac gacaaaccgt ttaaaaccgt tgccgaaggc gtaacggaat acatggcctg
    19501 gctgaatcgt gacgcataag agctctgcat gaaaatactg gtgatcggcc cgtcttgggt
    19561 tggcgacatg atgatgtcgc aaagtctcta tcgcacgctc caggcgcgct atccccaggc
    19621 gataatcgac gtgatggcac cggcatggtg ccgtccatta ttatcgcgga tgccggaagt
    19681 taacgaagct attcctatgc ctctcggtca cggagcgctg gaaatcggcg aacgccgcaa
    19741 actgggtcat agcctgcgcg aaaaacgtta cgaccgcgcc tacgttttac ccaactcctt
    19801 caaatccgca ttagtgcctt tcttcgcggg tattcctcat cgcaccggct ggcgcggcga
    19861 aatgcgctac ggtttactca acgatgtgcg tgtgctggat aaagaagcct ggccgctgat
    19921 ggtggaacgc tatgtggcgc tggcctatga caaaggtatt atgcgtaccg cacaagatct
    19981 gccgcagcca ttgttatggc cgcagttgca ggtgagcgaa ggtgaaaaat catatacctg
    20041 taatcaattt tcgctttcat cagaacgtcc gatgattggt ttttgcccgg gtgcggagtt
    20101 tggtccggca aaacgctggc cacactacca ctatgcggag ctggcaaagc aactgattga
    20161 tgaaggttat caggtggttc tgtttggctc ggcgaaagat catgaagcgg gcaatgagat
    20221 tcttgccgct ttgaataccg agcagcaggc atggtgtcgt aacctggcgg gggaaacaca
    20281 gcttgatcaa gcggttatcc tgattgcagc ctgtaaagcc attgttacta acgattctgg
    20341 cctgatgcac gttgcggcgg cgctcaatcg tccgctggtt gccctgtatg gtccgagtag
    20401 cccggacttc acaccgccgc tatcccataa agcgcgcgtg atccgcctga ttaccggcta
    20461 tcacaaagtg cgtaaaggtg acgctgcgga gggttatcac cagagcttga tcgacattac
    20521 tccccagcgc gtactggaag aactcaacgc gctattgtta caagaggaag cctgacggat
    20581 gcgggttttg atcgttaaaa catcgtcgat gggcgatgtt ctccatacgt tgcccgcact
    20641 cactgatgcc cagcaggcaa tcccagggat taagtttgac tgggtggtgg aagaagggtt
    20701 cgcacagatt ccttcctggc acgccgccgt tgagcgagtt attcctgtgg caatacgtcg
    20761 ctggcgtaaa gcctggttct cggcccccat aaaagctgaa cgcaaagcgt ttcgtgaagc
    20821 gctacaagca gagaactatg acgcagttat cgacgctcag gggctggtaa aaagcgcggc
    20881 gctggtgacg cgtctggcgc atggcgtaaa gcatggcatg gactggcaaa ccgctcgcga
    20941 acctttagcc agcctgtttt acaatcgtaa gcatcatatt gcaaaacagc agcacgccgt
    21001 agaacgcacc cgcgaactgt ttgccaaaag tctgggctac agcaaaccgc aaacccaggg
    21061 cgattatgct atcgcacagc atttcctgac gaacctgcct acagatgctg gcgaatatgc
    21121 cgtatttctt catgcgacga cccgtgatga taaacactgg ccggaagaac actggcgaga
    21181 attgattggt ttactggctg attcaggaat acggattaaa cttccgtggg gcgcgccgca
    21241 tgaggaagaa cgggcgaaac gactggcgga aggatttgct tatgttgaag tattgccgaa
    21301 gatgagtctg gaaggcgttg cccgcgtgct ggccggggct aaatttgtag tgtcggtgga
    21361 tacggggtta agccatttaa cggcggcact ggatagaccc aatatcacgg tttatggacc
    21421 taccgatccg ggattaattg gtgggtatgg gaagaatcag atggtttgta gggctccggg
    21481 gaatgagttg tctcaattga cagcaaatgc tgttaagcaa ttcattgaag aaaacgctga
    21541 aaaagctgcc atgatttaaa ttacaactct ttaatagata attttgaaat aaaatcagct
    21601 tccttgctga ttttatttca catattcatt acttatctaa taaacattgg tccgattgtg
    21661 ctttaaaata agcacaaagc aatattatgg tgacaaacac agcagtcgga accggtttag
    21721 aaaagagtat tacatcactt aggcctacta ccagtagtga aaaaagcaag attaaaatat
    21781 aaggttcttt agcagttctt aaggcatata tgaatattgc tgaaaaggct aaaattgtta
    21841 atgctactcc aggaatacca cgcgttgata acgtatctat gagatcgtta tgcaaatgag
    21901 aatctacaaa gggtaaagcg ccacttaatc taggctcttt ctcttctagt tcatgtattt
    21961 tttccgcacg tttttccagt gattgtccta ttggagaata tgtttttaaa ccaacttcat
    22021 acattgccag acgggcaccg actgaagttc tggtgttatc atgcgaataa ttaactaaat
    22081 ccctatttaa gtcattcatt ctcgtaagta atgtatcttt aaaagaaaat acacctgcta
    22141 ataatgcaat cgcaataaaa caatataact tataattaat tcgcttatta atataactat
    22201 ccattacgat taatagtaac gcaaagaatg gaaacactaa tatggctgca cgcgtttcag
    22261 ttactataac agcacataag ctaagcatga agttaattgt atataatgtt gttttatgtc
    22321 taaagtcgga ttttaaaatg agaattgatg ccagtaaaga agggaaaagg atgatgtagc
    22381 ctgctgttgt agcgtgggac gctgataatg caacacggtc gacgtttaaa tagaaatgtt
    22441 gccacatggc atgcgcaaaa aaaagtaact gcgttgccag ggatgcgaac agaattagtt
    22501 tcccaaattt tattttggtt ctcatctcat ctttagcaaa aagaactaag aaaataaaag
    22561 cactacacaa ggcaattttt ccagtttcca ttgggccacg atatgcattg gtataaaccg
    22621 agcctgaaac tttataatgt
//
LOCUS       DAZGHF010000077        22310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGHF010000077 DAZGHF010000000
VERSION     DAZGHF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.077"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..573
                     /locus_tag="ACHK3A_003056"
     CDS             <1..573
                     /locus_tag="ACHK3A_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8741108.1"
                     /translation="MLRLRRAGQITGQHVPEIILLNSHDGSSSYQMLPGYFRAICTNG
                     LVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQ
                     AALTYRYGNEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            912..1385
                     /locus_tag="ACHK3A_003057"
     CDS             912..1385
                     /locus_tag="ACHK3A_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8741109.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFSLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNVMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1401..1877
                     /locus_tag="ACHK3A_003058"
     CDS             1401..1877
                     /locus_tag="ACHK3A_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO8741110.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1946..2167
                     /locus_tag="ACHK3A_003059"
     CDS             1946..2167
                     /locus_tag="ACHK3A_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO8741111.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2330..2698
                     /locus_tag="ACHK3A_003060"
     CDS             2330..2698
                     /locus_tag="ACHK3A_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO8741112.1"
                     /translation="MSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            2788..3165
                     /locus_tag="ACHK3A_003061"
     CDS             2788..3165
                     /locus_tag="ACHK3A_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO8741113.1"
                     /translation="MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRFSTSPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKR"
     gene            3162..3650
                     /locus_tag="ACHK3A_003062"
     CDS             3162..3650
                     /locus_tag="ACHK3A_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO8741114.1"
                     /translation="MKLSLTLEADSINVRALNMGRIVVDVDGVELAELINVVCDNGHS
                     LRVVDESDRASTDCTPPFAALTGIRCSTAHITAKDSAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            3670..3867
                     /locus_tag="ACHK3A_003063"
     CDS             3670..3867
                     /locus_tag="ACHK3A_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO8741115.1"
                     /translation="MESLTTETALDILIAWLQNNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            3952..4818
                     /locus_tag="ACHK3A_003064"
     CDS             3952..4818
                     /locus_tag="ACHK3A_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8741116.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMQSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHRSKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISGMQADRLADLERMLHLLSGKPIPDNR
                     GNITINLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            4890..5153
                     /locus_tag="ACHK3A_003065"
     CDS             4890..5153
                     /locus_tag="ACHK3A_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO8741117.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            5150..5476
                     /locus_tag="ACHK3A_003066"
     CDS             5150..5476
                     /locus_tag="ACHK3A_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8741118.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(5890..6426)
                     /gene="yghD"
                     /locus_tag="ACHK3A_003067"
     CDS             complement(5890..6426)
                     /gene="yghD"
                     /locus_tag="ACHK3A_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO8741119.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(6428..7606)
                     /gene="gspL"
                     /locus_tag="ACHK3A_003068"
     CDS             complement(6428..7606)
                     /gene="gspL"
                     /locus_tag="ACHK3A_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO8741120.1"
                     /translation="MSSMLEIFIPLCASDPIRWQHRTTDVEHGIWSDVDNEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGPDAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(7603..8580)
                     /gene="gspK"
                     /locus_tag="ACHK3A_003069"
     CDS             complement(7603..8580)
                     /gene="gspK"
                     /locus_tag="ACHK3A_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO8741121.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVAQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(8577..9182)
                     /gene="gspJ"
                     /locus_tag="ACHK3A_003070"
     CDS             complement(8577..9182)
                     /gene="gspJ"
                     /locus_tag="ACHK3A_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO8741122.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(9179..9550)
                     /gene="gspI"
                     /locus_tag="ACHK3A_003071"
     CDS             complement(9179..9550)
                     /gene="gspI"
                     /locus_tag="ACHK3A_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO8741123.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(9547..10110)
                     /gene="gspH"
                     /locus_tag="ACHK3A_003072"
     CDS             complement(9547..10110)
                     /gene="gspH"
                     /locus_tag="ACHK3A_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO8741124.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQAF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYA
                     SAQNACWAVKLAHDGVLSLNQCDEGMP"
     gene            complement(10114..10569)
                     /gene="gspG"
                     /locus_tag="ACHK3A_003073"
     CDS             complement(10114..10569)
                     /gene="gspG"
                     /locus_tag="ACHK3A_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO8741125.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(10586..11809)
                     /gene="gspF"
                     /locus_tag="ACHK3A_003074"
     CDS             complement(10586..11809)
                     /gene="gspF"
                     /locus_tag="ACHK3A_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO8741126.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(11809..13302)
                     /gene="gspE"
                     /locus_tag="ACHK3A_003075"
     CDS             complement(11809..13302)
                     /gene="gspE"
                     /locus_tag="ACHK3A_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO8741127.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTVQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(13302..15362)
                     /gene="gspD"
                     /locus_tag="ACHK3A_003076"
     CDS             complement(13302..15362)
                     /gene="gspD"
                     /locus_tag="ACHK3A_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO8741128.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDDQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(15392..16351)
                     /gene="gspC"
                     /locus_tag="ACHK3A_003077"
     CDS             complement(15392..16351)
                     /gene="gspC"
                     /locus_tag="ACHK3A_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO8741129.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHILSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTVVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFREGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(16369..16779)
                     /gene="gspS2"
                     /locus_tag="ACHK3A_003078"
     CDS             complement(16369..16779)
                     /gene="gspS2"
                     /locus_tag="ACHK3A_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO8741130.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(16845..17654)
                     /gene="pppA"
                     /locus_tag="ACHK3A_003079"
     CDS             complement(16845..17654)
                     /gene="pppA"
                     /locus_tag="ACHK3A_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO8741131.1"
                     /translation="MFFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLKALF"
     gene            complement(17784..22310)
                     /gene="sslE"
                     /locus_tag="ACHK3A_003080"
                     /pseudo
     CDS             complement(17784..22310)
                     /gene="sslE"
                     /locus_tag="ACHK3A_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
BASE COUNT         5068 a         6296 c         5656 g         5290 t
ORIGIN      
        1 atgctgcgtc tgcggcgggc cggacagata accggtcagc atgtgcctga aattattctg
       61 ctcaactccc atgacggttc atccagctac cagatgttac ccggatattt tcgtgccatc
      121 tgcaccaatg gcctggtctg cggtcagtcg ctgggagaag tccgggtgcc acaccgggga
      181 aacgtggtgg acagggtgat tgaaggggct tacgaggtgg tgggcgtttt tgaccggata
      241 gaggagaagc gtgatgccat gcagtcgctg attctgccgc caccggcacg ccaggcgctg
      301 gcacaggcgg cactgactta ccgttatggt aatgagcatc agcccgtcac caccgctgat
      361 attctgacac cgcgacgccg ggaggattac ggtaaggacc tgtggagtgc ttatcagacc
      421 atacaggaga atatgctgaa aggcgggatt tccggccgca gtgcaaaagg aaaacgtatc
      481 cacacccgtg ccattcacag catcgacacc gatattaagc tcaaccgcgc attgtgggta
      541 atggcagaaa cgctgctgga gagcatgcgc tgataaaaca tatccctgaa ggcgtgattc
      601 aggtaccaga atcacccgac gccccggagg cgctgccggg gtgtcgttcc atttctgatg
      661 atgacctgtg cacctggtgt acccgtctgc tttaccgtcc cggagaactc agtctgtgca
      721 gactgagtac aggcaatggc atctggccat ccgtctgtga ccggaacggt tacgcgtacg
      781 gctgtccgga atttcagccg aatatcattc atccctgata ttgccgggtc tcacggtcag
      841 tgatgaacgt gatgttgtca gcccccggcc tttatttcct ctcatttatt cacattacaa
      901 ggatttcaca catgaaaaca ttgtctcaga acaccacctc ttcagcctgt gcaccggaga
      961 ctggcctgca acaactggtt gccaccatag tccctgatga acagcgcatc agcttctggc
     1021 cgcagcattt tagcctcatt ccacagtggg tgaccctgga gccccgtgtc ttcggctgga
     1081 tggaccgttt gtgcgaagac tactgcggtg gtatctggaa tctgtacacc ctgaacaacg
     1141 gcggcgcatt tatggcccct gaaccggatg acgatgatga cgaaacatgg gtgctgttca
     1201 atgtcatgaa cggtaaccgc gctgaaatga gtccggaagc cgccggcatt gcggcctgtc
     1261 tgatgacgta cagtcatcac gcctgtcgta cggagtgtta tgccatgacg gtccactatt
     1321 accggctgcg ggattacgcc ctgcagcatc cggaatgcag cgccattatg cgcatcattg
     1381 actgaatgga ggcagggaca atgcagcagc tttcctttct gcccggagag atgacgcccg
     1441 gcgagcgcag tctcattctg cgggccctga aaaccctgga ccgccatctt catgaacccg
     1501 gcgtggcctt cacctccacc cgtgcggcac gggaatggct gattctgaac atggcaggac
     1561 tggagcgtga agagttccgg gtgctgtatc tgaacaacca gaatcagttg attgccggtg
     1621 aaaccctctt cacaggcacc atcaaccgca cagaagtcca tccccgggag gtgattaaac
     1681 gcgccctgta ccacaatgcc gccgccgtgg tgctggcgca caatcacccg tccggtgaag
     1741 tcacccccag taaggcagac cgccttatca ccgaacgtct ggtacaggca ctgggcctgg
     1801 tggatatccg ggtgccggac catctgatag tcggtggcag ccaggttttc tcctttgccg
     1861 aacatggtct gctttaaccc gtcaccgcca caatcacatc acacctgttt tcacttttat
     1921 cttctgtctt cagaggtatc ccattatgaa aattatcacc cgtggtgaag ccatgcgtat
     1981 tcaccaacag catcctgcat cccgtctatt tccgttctgc accggtaagt accgctggca
     2041 cggcagtgct gaggcgtata ccggtcgtga agtgcaggat attcccggtg tgctggccgt
     2101 gtttgctgaa cgccgtaagg acagttttgg cccgtatgtc cggctgatga gcgtcactct
     2161 gaactaatgt tccgtcaccc ggaaaacgag tacgaccaga tgacctcaga cgccgctgtg
     2221 gatgaggccc ttctcatcaa tgaatacatc ttcaccgaag ccggtctcca caccggctga
     2281 cctgtcatcg ttaacccctt cttaaccgat tttctgtgag gactttatcg tgtcagacac
     2341 gcaccatgag acaaattatc ccgacgacta taacaaccgc ccctggtggg ggctgccctg
     2401 caccataaca ccctgttttg gcgcccgtct ggtgcaggag ggtaaccggt tgtattacct
     2461 tgccgaccgc gccggtatca gaggccggtt cagcgacgcg gatgcgtacc atctggacca
     2521 ggcctttccg ctgctgatga aacaactgga actcatgctc accagcggtg aactgaatcc
     2581 ccgccatcag cataccgtca cgctgtatgc cagaggactg acctgcgaag ccgacaccct
     2641 cggctcctgt ggttacgttt atatggctgt ttatccgacg cccgaaacga aaaagtaaat
     2701 catcagaata accttctgct ccggcctggt gttttcacca cgccactttc ccatttttca
     2761 tctctgcata tcaggaaaat cttcagtatg aacacattac ccgacacaca cgtacgggag
     2821 gcatcgcgct gcccatctcc agtaaccatc tggcagacac tgctcacccg actgctggac
     2881 cagcactacg gcctcacgct aaatgacaca ccgttctccg atgaacgtgt gattgagcag
     2941 catattgagg caggcatttc actgtgtgac gcggtgaact ttctcgttga aaaatacgcg
     3001 ctggtgcgta ccgaccagcc ccggttcagc actagtcccc gctctcagtt aataaacagt
     3061 attgatatcc tccgggctcg ccgggcgacc ggcctgatga cccgcgacaa ttacagaacg
     3121 gtaaataaca ttacccgggg taagcatccg gaggcgaaac gatgaaactt tccctgacgc
     3181 tggaagccga cagcattaac gtgcgggcgc tgaacatggg gcgcattgtc gttgacgtcg
     3241 atggtgttga gcttgctgaa ctgattaacg tggtctgcga taacggtcac tcccttcgtg
     3301 ttgttgatga atctgaccgg gcctcaacag actgcacgcc accatttgct gccctgaccg
     3361 gcatacgctg cagtaccgcg catatcacgg caaaagacag cgcctggctg tactcgctgt
     3421 cacatcagac gaatgacttc ggcgaatcag aatggattca ttttaccggg tccggttatc
     3481 tgttacgtac tgatgcgtgg tcgtacccgg ttctgcgact taaacgcctg ggcctgtcaa
     3541 aaacgtttcg tcgtctggtc gtaacgctca tccgccgtta tggcgtcagt ctcattcatc
     3601 tggatgccag cgctgaatgt ctgccgggtt tacccacctt caactggtaa ccgggacaaa
     3661 agacacagta tggaatcatt aaccacagaa acggcgctgg atattctgat tgcgtggcta
     3721 cagaacaata tcgactgcga atccggaatt atcttcgaca acgatgagga taaaacggat
     3781 tcggcagcac tgctgccctg cattgaacag gccagggaag atatccgtac actgcgccaa
     3841 ctgcagtttc tgcaacagaa ccggtgagtc ttactcacca gacttcattc cactgacgcc
     3901 agcctgaata cggctggcgt tttcgtttat aaaccaaaag gaaaaacgat gatgtcagat
     3961 atcacagcct cccgtccgga agtggtcacc gggcatacgg acgttatttg ctcaaccagt
     4021 atccgccaca ttctggctgt acgaaagagt acactgctgc aaatcgacac acttatccgg
     4081 caactggctg aaatctcagc aatgacagaa agtattggcg gtaaaaccgc actggactgg
     4141 gcgatgaaac aggattttcg ctgcggctgc tggctgatgg agaaaccgga aaccgcaatg
     4201 aaagccatca cacgcaatct cgatcgcgaa atctggcgtg acctgatgca acgttccggg
     4261 atgcagagtc tgatggatgc gcagacccgg gacacatggt acaggtcact ggaatatgat
     4321 aattttccgg aaatcagtga agcgaacatt ctgagcacat ttgaacaact gcaccggagt
     4381 aaggatgaag tgtttgagcg gggagtgatc aacgtcttca aagggctgag ctgggattac
     4441 aaaaccaact caccctgtaa atttggcagt aaaattatcg tcaacaatct ggtgaggtgg
     4501 gaccagtggg gatttcatct tatcagtgga atgcaggcag atcgcctggc tgacctggaa
     4561 agaatgttgc atctgctcag cggtaaaccg atccccgaca accgggggaa tatcaccatt
     4621 aatctggatg accacataca gtctgttcag ggtaaagagg actatgaaga cgagatgttc
     4681 agtattaaat actttaagaa aggctcagta cacatcacgt tcaggaaacc agaactggtt
     4741 gacaggctta atgatattat tgccagacac tacccggaag ccctggcaag cacagtgaac
     4801 aaaagccgca aggcataaaa gaggtatttt acaattcgcg aatattaact attattaatt
     4861 gatagcaatc aatcggcgag aaaatcacca tgcctaccag tgtatccctc agtccttact
     4921 ttgaaacgtt tatccgcgaa caaatagaaa gtggtcggta taacaataca agtgaagtca
     4981 ttcgtgccgg acttcgtgct ctggaagaac gggaacagca gataaagctt gaatcactcc
     5041 agagcgccgt taccgcaggc atcaacagcg gtgaaagtaa aagtgctgaa gaggtattcg
     5101 ggcgactgac gcacaaatac aaaaagatgg cagaaggtga acagccaata tgaaactgac
     5161 tgtttcacca ttagcagaac acgatcttga agctataggt gactggatcg ctcaggataa
     5221 tcctgtccgt gcaatcagtt ttaccgaaga gctttaccaa caatgtctgt tgatagccga
     5281 atcgccagta ctctacagag aaagacccga attaggggct gggatcagag gttgttcgta
     5341 tggtcgttac cttctgctgt tcagggtact ggatactgaa gttcgtatcg aacgtatcgt
     5401 tcatggttcc cgggatatca acagggtact tgcagagcca gacgacaaac catcagatca
     5461 tttctggcag ccataaacaa aatctgtgcg ttaacgctcc ctaacacagg tagccaggta
     5521 ttttataaat ctctgtattt tgttgttact tcgattccgc aattcagcat atcgaaccag
     5581 acaaaatgat tacagcaaag cacaaaaaag agggattata gcgtcaaaag aattcgaatt
     5641 gcacatgggg gcctcattgg tggcctcaaa aacaacaaag acaaaacaat cgatacaaaa
     5701 caattggtta tataaccagt ttcattccga gtccgggcac cactattcta aagaacccgc
     5761 tcacaaggcg ggtttttgct tttggatctg acaataacct tcacgaaaca aattagctta
     5821 taaagcctgg gggaattact ctcgccacgt taacgagagt aattttattg atattaatct
     5881 cctgatactt tatccccgtc caaactccag ccgctgcaca ttcaccatcc cagtcttctc
     5941 agcagcactg acatcaattt gtgtcacccg cagcgcatat ttttcatcca gtgcgtttaa
     6001 ccatttcagc aggtcattaa acaccacagg ttctatccag acctgaatat tctccccacg
     6061 ctcggctatc cgcttgatga ccaccgagtg cgcggaagca ctgtcactga tgacccgcga
     6121 tacctgcgca ggcgttgttg tggcagattt tcgcgctgca ataatatccg gcgcggcgtt
     6181 cttcagtcgg gtgttcattg ccaccagctg ctgcaacatc gtctcctgtt gttctatccg
     6241 ttcgctcaac ggctgccaga tgaggacgta atatccgacg ctaaacagga acaccaccgc
     6301 tgccagtaac atgccctttt cacgcggaga acgccccgcc agatgttgtg tcagccagtg
     6361 ttcgccacgg cttaactggc gttcacgcca ttgctgaaaa tagtgaataa atttatcgcg
     6421 taacatgtta tttcctccgc aacgttacgc cgccggaaac cgcatcaccc tctttctgta
     6481 acgcgtcctg ttgcacaaca taatctgccg ccagtgcgct acgcagtttg tcgaagctgg
     6541 caaagttcgc agcccgtagc tggaggtgaa gcgtctggcg tttttgatca aaggtgaaac
     6601 cacgcatttc gatgtcggta agtgacgctg atttcagggt actggcgatc gctgacaatt
     6661 ctgcgagcag ccgggtatcg tcggcctgcg ggcgatattt tttcagcgcc atcgtcacct
     6721 gagagcgtaa attcacaatc cgcttctgct ccgggaacag tgttaagaac tgtttctccg
     6781 cctgggtgcg gctttgcgcc acctgttcgc tgacgctcca taacgtcacg ccccgttcca
     6841 ctgccagcgc aaccagaatc agcaatatcg gcagaatcat cacccgccag cgcgcccact
     6901 gttttcggta gctgacgcgg ggctgccacg gccctgttag caggttccct tccggttcgc
     6961 cataagtggt aatggcgggc agagccgtaa cggtcaggcg ttcagcatct gggccagccc
     7021 atgctgatag ctcttccggt gcaatgccga ccacggttag tgaaagcggt aaatcctgct
     7081 cattgagctg tgcgcggaac atgaccggag ccagcgccag cccggcgctc catccccggc
     7141 attcatcgat gcggcagata acccgttgcg catcgccagc cataaaccca caaggaatgg
     7201 acatccagtc tggcgcgacg gtagcgcggg tgatgccgtt tgcctgcaac cactgcgcaa
     7261 tgttgcgcat atgctgttgg tgaatcacag ctacggttgc cagttgctgg tcgattttca
     7321 acggggcgaa atgcagttca tcgatatcct ggttcagctc ttcttccagt aaggcgggca
     7381 gaatagtcgg tatctgcttg cgggcgacat caggcagttc aacctgccag acgctgatcc
     7441 attcgccggg aatgtagagt cgaatcgcat cagtttgcag ccattgctgg agctgttcgt
     7501 tgtcaacgtc agaccagatg ccgtgctcca cgtcggtggt acggtgctgc caacggatgg
     7561 gatcggaggc gcaaagcggg ataaaaatct caagcatgga actcactcac tttctcctgt
     7621 ctgatgccag agaaccgaaa agtgttgtgg gcccatgcgg acaattaacg agttcatcgt
     7681 cagttcaatc tcattcacgg tgatatctga acgcagccag aagtaattgc tgtccacgct
     7741 caggacggtt tttagctgtt ttttagtacg ctcatcgacg tcagcaagta gcggctgtgc
     7801 aagaaactga tcgacatctt cccagccctt cgccggacgt tgttgtaata acgcccgcgc
     7861 ctgaacaggg cttaaccacg ggtcaaacag cgcctcaaga atcacacttt gcgcgacgtc
     7921 cagggtatta atgttgattt gctggcgggt catcggcagc gcacagacca gcggtttcag
     7981 tttttgataa agtccggcgt ccattccctg caccacgcgc atctcgctga tatcagccag
     8041 cggttgatta gcggcgtaaa acggcaccga acgggcgaga tactcgctgt cttcacggcc
     8101 cagacgcgtc tgcacgctgc ggtcttcgtc aataaactcc cacaggcttt cggctatcag
     8161 ttcggcccga taagcaggca catccaggcg cgtgatcagg gcaatcagtt gttgtaccgc
     8221 gagcggacgc gacgccgtcg tcggctgagc gagagcattc aggttaaagc aggcctgtgc
     8281 gtcacgcaga gtgacggcga tttgcccctg cggcagtggg aaaaaacgag ggccggaagc
     8341 ccacggctgc gccagatgca cgcgcttttc atttttcagg ctcagactga gtgcgctcaa
     8401 cgccaggctt tccgcactgg cgctgtacca cagcgcctgc tggtactcct gctggctgcg
     8461 cgttcgccca agttgttgct gcattcgccc ggaaagcgtg atggtcacca gcatcataac
     8521 cgccagcaat accagcacca cgaccagtgc cattccgcgt tttggtggtg aggtgatcat
     8581 gataattgcg gcccgcgtaa caaccagatg cgttcaatct cgccccattg cggcgaatgc
     8641 agagtcatgc gcaccgccac tggtatcgcc tgcactgatg accagctctc ctgccagcgc
     8701 gtgccgtcgt agaactgcaa acggagcgaa tccgccggga ttaatttttg cgttgttggc
     8761 ttcacgctgc ctgccgcatc ggtcagtggc caggctaacc gttcgagata accaccatga
     8821 atgcggtaac cgacggtgag cagattactg cgcggcagac gcatcaacgg attaaccacg
     8881 ccgccgcgca caaaacgcat cccttcactc tcagacgcca gcacgccagc gcccgccagt
     8941 aacgctggtt cacgctggcc ctgatcgcct cttaccggac gcggcatcat ttgtgtcaga
     9001 tcgtggttca aaaaactcat cgtttgctgc atgaggttta gtttttgatc gtgtccggcg
     9061 acggcgctat tcacgcgtgt aacgccgttt gtcacctgct gcgccatcag tgccagtgag
     9121 gcaaaaatgg caattgccac cagcatttcc agtaacgtga aaccagcgca agtccttctc
     9181 actgttggcc tcccacggcg ctaaaccagg cgcgtcgtga ctgaatcacc gacgaaaagt
     9241 cttcgtgacg gctgacttca atatccactg cctggagcag cgcgttgcct gtactcagcg
     9301 gtgtggttcg ccagtaccag cggcttcctg ccatatcact ctcgccctgg tgcctgaccg
     9361 ctcgttgttc acgggtcatc aggtaaagca gtgcggtctg gttttcagct acccagcccg
     9421 ctaccgtttt ttcttcaagg atttgctgat tactcagcgc gccgctggcg atctgtaaca
     9481 ccgccatggc ggccaacgca aaaatcgcca gcgcgagcat tacttccagc aaggtaaatc
     9541 cacgcttcat ggcatcccct catcacattg attgagggat aacacgccat cgtgcgccag
     9601 ctttaccgcc caacacgcgt tttgtgccga cgcgtaaaaa cgcagtgtaa acggcgtggc
     9661 gggttcaaaa ggcgaaaaac ggatctgcgg tgtcttcttt ttcgcctctt tttgcagctc
     9721 cagttcaata tcgtgcagcg tcaggcgacg acgctgcagc tccagcgcat attccttctg
     9781 ccagatatca cttccgggtt gtaacagcat ctgcacctgt ttcggcaccg taacctgtgc
     9841 cgataaccgg gtcgcagaaa cgggcaacca ctgcccgtga cgacgctgca taaactggta
     9901 acctggtggg tcgattagca caccgagtgt tttcccttcg atcactgccc tgtccttaaa
     9961 ctgcgcaaag cgagtcagaa aagcctgcgc cgcttttttc gcaggcggct ctgaagcggt
    10021 cgcaaacgtc tgtaccacgc ccgcactggc aaggccgata aggaaaatca ccagcatgat
    10081 ttccagaagt gtgaatccgc gttcaggcac tgattactga aactcctgca aattccagtt
    10141 accgatatct gcgccagcgc cctccccgtt ttcctgacca tctgccccca gggtatacac
    10201 atcaaacagt cctttttcac ccgggctgag atactgataa tcattgcccc acggatcttt
    10261 tggcagtcgt ttaatgtatc caccggtacg gtagttgcgg gagtccgcca tattggccgg
    10321 ttgctggatc agcgcctcca gtccctgttc ggtagtcgga taacgcccgt tatccagtcg
    10381 gtacatatcc agcgcattct ccagcgccac gatatcgctg atggcttttt gccgatcggc
    10441 tttctctttg ttgcccagca ggttaggcac caccagactt gccaggacgc caagaataac
    10501 aatcaccacc atcacttcca gcagggtaaa acctgcccgt ggtttttgtg tgcgggataa
    10561 cgaattcatg tgataactcc gtaaattaca ttccaaccat attgttcagt tgcagcatcg
    10621 gttcgaggat ggcgatgacg ataaacagca ccacgcccgc catcatcacc accagcgccg
    10681 gctcaaacag ccctaacgcc agccccacct gggtatcaaa ttctcgttcc tggttaactg
    10741 cggcctgttc aagcatggtt tccagttcac cgctctgttc gccggaggcg atcatgtaca
    10801 gcatcatcgg tgggaacagg cgcaactccg ccagcgcagc gcgcagactg cttccttcgc
    10861 ggacgcgatc tgccgccagc agcagttgtt gttcgacata gcgatttgcc gacacggcgg
    10921 cagcggtctg aatgccttcc agtagcggaa cactgctggc ggtgaggatg cttaacgtgc
    10981 gggaaaaacg cgccgtattc agcccgcgcg caacacgccc cgtcacgggc aaacgcagca
    11041 aggttttatc ccagcgtagg cgcatggcag gatttttgag tagccgttgc cccagcacca
    11101 gaagccccag caatcccgcc agccagtaaa cgccgctggt ctgtaacgtg tcgctcatgg
    11161 cgatgagcgt gcgggtggag gcaggcagcg cgtgtccgag atgatcaaac tgttcgatga
    11221 ttttcggcac cactgccgtc agcaaaatag tgactacgcc cgttgccacc accagcaata
    11281 ccagcggata gagcatggcc tgcaacagtc gtgatttcag gcgctgtcgc tgttcggtgt
    11341 aatccgcaag gcgattgagc accacgtcga gatgcccgga tttttctccg gcagcgacca
    11401 tcgaacaaaa cagggaatca aagacgcggg gatgttcgcg caggctgtcc gacagggtat
    11461 aaccttcctg aatccggctg cgcagcgcca ttccgaggct tttcacatgc agtttttcac
    11521 tttgctcact gaccgcctgt aagcaggttt ccagcggcat tgctgcctgc accagcgttg
    11581 ccagttggcg cgtgaacagc gccaaatctg ccgccgccac gcgacgatgt gcgtgccgcc
    11641 gacgctgcaa cattccccct gtcgaagcat tcagccgggc ttcaatgtgc acggggataa
    11701 gctctttacc acgcaacaac tggcgggcat gacgcgcgga atccgcctca atcattcctt
    11761 tggttttgcg accattacgc tccagcgcct gatagtaaaa cagtgccatt acgcctccat
    11821 agttacccgc agaacttcat cgagagaggt ttctccggcg agcactttct caatgccgtt
    11881 gctgcggatg cccgcagagt gttgccggac ataacgttcc agctccagtt ccccggcctg
    11941 acggtggatc aaatcacgca atgtggcgtc caccacgatc aactcatgga tggcagtacg
    12001 cccgcggaaa cctttgtgat tacaggcggg gcagccctgc ggatggtaca gcgtgacagt
    12061 acgggcgtcg gtaatcccca gcaggcgttt ttcttcgtca gtggcaggcg cgggctggcg
    12121 gcagtcgggg cacagcgtgc ggaccagtcg ctgcgccatc acgcccgtca ggctggaaga
    12181 gagcaggaaa ggctcaacgc ccatatcctg cagacgtgtg atcgccccca ccgccgtgtt
    12241 ggtatgcagc gtggaaagta ccaggtgccc cgtgagcgac gcctgaacag cgatttctgc
    12301 ggtttctgta tcgcggattt caccgaccat caccacatcc gggtcctgac gcaaaatcgc
    12361 gcgcaggcca cgggcaaagg tcatgccgac gcgggtgttg acctgcgtct gaccgatccc
    12421 ttcaatcatg tattcgatgg ggtcttcaac cgtgagaatg ttgcgcgaat ggttgttcag
    12481 ctcctgcaat ccggcgtaca gcgtggtgct tttgccggaa cccgtcggtc ccgtcaccag
    12541 aaagatgccg tgcggtttgt gtaacagctg gcgcaactgc acggtcagtt gctgactcaa
    12601 ccccagacgt tccagcgtca ggcgggcctg gtttttgtcc agcagtcgca gcaccacgcg
    12661 ctcgccccag gcggagggca tggtggagac acgcacgtca atcgcccgac cgcccagcag
    12721 cagcgcaata cgcccatcct gcggtacgcg cttttcggca atatccagcc gcgccatcac
    12781 cttgatacgc gataccagca gcgaggccag tttgcgcccc gggcgcagca tttcatgtaa
    12841 tgtgccgtca acacgaaaac ggatcaccag actcttttca aatgtctcga tgtggatatc
    12901 cgaagcgcct tctttaatcg cctctgccag catggcgttg atcagtttga tgattggcgc
    12961 gtcgtcgtcg ctttccagca gatcttccgt ttccggcagt tcttcggcga gggtaaaaaa
    13021 atcctcagca gaaccgagat cttccatcag ctggcgggcc tcggaagagt cccgctgcca
    13081 gaccgcattc agccgctgtt caaattcggc ttcgtcgatt tgccgcagcg taaagggcgc
    13141 gttcagcccc cgctgcagct cctgcaaaac agagagcgac aacggatgaa cgtggaggat
    13201 ctccagcgac gcttcgcacc atgccaccag gctaaaccga cggctgaaac tgtagggcag
    13261 acgcacggtg ttagcggtgg tttcctgtgc tacaggcacc attaacgcgt tctcccggca
    13321 ttgaggaatg cgcgaacttc cggcggtaag gcctggtttt gcgctggcag taccggctgc
    13381 gcggtgtgcg gcatcaggct taagccttga tcatcgcgat agatctgctc ggcgcgcatg
    13441 tagttatatt tgcgctgcga cacgccgtct gccgccatac cgtcacgcag aatggtcggg
    13501 cggataaaca ccatcaggtt acgtttttct tttttatccg ccgtcgattt aaacaggtta
    13561 ccaatcaacg ggatatcgcc cagcagcggc actttcgcca cgctttctcc cgcctggtcg
    13621 tccatcagac cgccaagcac gatcagttca ccatcgttag ccagcacggt ggtcttcagt
    13681 ttgcgctcac caaacaccac gtcgaggctg gtctgtcctt ccaccttcga cacttcctgc
    13741 tcaatcacca tctgtaccgc gtttccttcg ttaatctgcg gcgtgacttt cagcatgatg
    13801 ccgacttttt tcctctctac cgtattgaaa ggattgctgt tattggagcc aacggtagat
    13861 ccagttaata ccggaacgtc ctggcccacc atgaagaagg cttcctggtt gtccagcgtg
    13921 gtgatgctcg gcgtggagag cacgttcgag ctggagtcgt ttttgaccgc ctgtaccagt
    13981 gccatccagt cgcctttcac cacgccaacc gccgtaccgc taaagccaga aagcagctgg
    14041 gcgagcgtgg agagatcgcc gttggtatcc gggttaatgg tggtggcacc gttttcactg
    14101 atcaccgtgg agcctttctg cggtttcgcc tgagaaatgg ctgcccccag cgtgccgata
    14161 gggatctgcg taccgttagc aaactgcatt aatccggcat ctttcgacgc ccactgcacg
    14221 ccgaaattga tattgctgcc ttcagcaact tccacgatca acgcctcgac gtgcacctga
    14281 gcacggcgaa tatccagttg ttcaatcacg ctttgcagcg actgcatgat gtcctgcggc
    14341 gcagtaacaa tcagggcatt actgtgtttg ctggcggcga tggagacaac ctcacgcccg
    14401 ctaccgacgg tgccttccgc ctcttcttta gccgccgtga gcgtgccgct gacctgcttc
    14461 agcacatcga ccagatcttc cgctttgctg tatttgagat agaaaacctg gctgttgccg
    14521 ctgcgctcca tttctgagtc cagccgacgg atcaggcggc gcattttgtc ccgcgtggcc
    14581 gggtcaccac tgacaatcac actgttggtg cgttcgtcgg cgacaatttg agatttcagc
    14641 gtcgcaggtt ggttctcgcc gctgtttttc gtcaggcttt ccagcacgcg ggcgatttcc
    14701 gaagccgagg cgttatccag cgggatcacc tcttcagtgc gattacccgc gtgatccaca
    14761 cgctggatca cttccgtcag ccgctccacg acggaggcgc gcccggtgag cataatcacg
    14821 ttggagggat cgtaattaac aacgttgcct gagcctgcgc tgtcaatcat ctggcgcaga
    14881 atcggtgcca gctcgcgtac cgacacattg cgtaccggca cgactttggt aaccatttca
    14941 tcgcccgcgt agttgtcgct gccttcaccg accagcggca gcggctcgac tttcgcggcg
    15001 cttgatttca ccaccttcag cacgtcgttt tccatcggta cgaccgcata cccctgtgct
    15061 tccagcaggt taaggaataa ctggtaatac tggcgttcat tgagcggagt catagtgcga
    15121 atactcactt tcccctgaac gcccggcccc ataatgatgg ttttattaag gttagcgccg
    15181 acggtttcga tgaacgattt caggtcagta tctttaaaat tagcggtgaa agtggcttct
    15241 tccgcccaga ccggtgaact acacaatgcc gctgccagca tcagcggcag taaacgcttt
    15301 tttgttttga ggccagttgt cttcttacgc cagatagaca acgtcatatc acgccaaaac
    15361 acgatgaatg attctcctgg attaaatgcg gttagcgcag tgcgatggaa atgtcgtagc
    15421 gcgcaccctt gcgtaaaacc gtaagttgaa tggaatccat tgaaggtaac tgccgcatca
    15481 gagcaatcat tgctcgtgga tcagtgaaat cctgctgatt tagtgcaatg gcgatatcgc
    15541 cttccctgaa accgctggca tcgaacagag aacgatctgc ccccggcttc actgcataac
    15601 cgacaattcc ctccttacgc acaggcgtaa gctggatata gttaaaaatt ttctgcggat
    15661 ctttcgccag tgcctgacgc acggcagccg ggatctcaac tggcgcactg gcagcaggct
    15721 cagcaacagc ttgctttgct tcgtcgctga cggctttttt gctggtgacc gccacagggg
    15781 ggcgttcctc ctctgccaga ctcagacgtt ccattttccc ctgatagcgc agcatcacat
    15841 ggtcgcggtt gatttcctca atcactgcgt tgtgagagcc aagccgttca ccctgcaaat
    15901 agacctgctg tttgccgcct tcttcaatca ccgcgccggg tctggcacca aaggcgatcc
    15961 cacgcagcac cacattaaga cgcgtttctg ccacaggcgc agattcaggt tgttttaccg
    16021 gtgcggcgac gggttgatat ttgccaaacc agttttgttg gctgactaat tgcacgtcat
    16081 ttttatcgaa cggttttgca tccgcacgga gcggtttatt cgccgatgag gaaaccaccg
    16141 tatattccgc agaaaaggag aaatagcgcc agagtgaata cgccattttt gcagaaataa
    16201 taagcagcat gagccagaac atcccgcgca ccatttttcg cagatgctct ttgtctgtat
    16261 taagagattg tctctggcta agaatgtgac gaattttagt cagccattga atgagataaa
    16321 ttcgtgcgtc acgaaaaaca acccgcgcca atttatatct acccgacgtt atgctttgac
    16381 tattccacag gtggtacgat ccagtttccg ctgatactgg ttacctgtac gtgtatcatt
    16441 aatcgttatg ctgtaattaa ttccctcggt gatcattaat ttcaccgttg ggtcagcgca
    16501 catttgtcgt tgatagctgg ttaaaaaggc gtcaggtgtc tgcgtggtct gagtacccga
    16561 ttcgctgata atggtcattt ttaccgtcgt gccactactt tgcgccagca ctaaggtata
    16621 tcccgcagat ttaatcggca ggttttggct gatattttgc gcctgttttt tcgccagtaa
    16681 actggcgttt tcattatggc tggcgcagcc acttaataat cctgtaacgc tcaacaatag
    16741 cgatattaat accctccctg gcatttgttt tatcgacatg gtaaataatc tctaaggtta
    16801 ttaataagag ttaaaatgtc actttgataa tgacgttgtt atcattaaaa caatgccttt
    16861 agataaagtg ttgctatacc gcccagactt aaacacggtc caaaaggcag tgtggttgat
    16921 cctctttttg taataacggc atatatcagg ccgcagcatg aggcgattaa agcaacgttg
    16981 ggcagtgata acgcccccac ccagccacct aaagcggcga agagtaatac atcgcccatg
    17041 cctaatgctt ctttacgcag aactattccg gctatccagc gcagggagta aaaagtgata
    17101 aatcccacca gtacgccggt gactgcgtct tgtagcgtga gcggactctg ctgcgcccat
    17161 gccgcaatta gtcccgtcca caatacgccc tgagtaaaaa catcgggcag ccattggtta
    17221 tcgaggtcaa tgacgctcgc ggcaatcagc caggcggata atatcatcac cgccagcgcc
    17281 catccacttt ccggccacac caggctcgcc agcaaaaaag cgagtgccgt caataactcc
    17341 accagcggat aacgcttgct gattttcgcc tgacagtcgc ggcagcgccc tttgagcatc
    17401 aaccaggaga gcagcggaat attgtcacga acgcggatag tctgctggca atgcggacag
    17461 tgcgagcgtg gcagcgccag gcttattttt gactgcgtac tcggcatttc accgtgaaac
    17521 tccgccattt gttggcgcag catgatgggg taacgccaaa tcaccacatt caaaaaactg
    17581 ccgataatca atcctccgac ggtagccagg acgggcatcg ccgcggggta ttgctgaaaa
    17641 acatcaaaaa acatggttaa aggttatttg ttgtaacttg ccggatgcgg gcctgcaggt
    17701 gtatgccata cggctttcct ccaggcccga tgcgccttat ttcatgccgg atgcggcgcg
    17761 agcgccttat ccggtataca ggcttactca gctgacatct tatgctcggt aacctgatta
    17821 atggtttccg gcccttgctg cggtttcggc agattaagcg ccgccagcgt ctcgtaagcc
    17881 gactggctca caccgccctc gaagttcatc tcgctcgctc ccggcaactg gtaagcattc
    17941 gcgcccggat tccatttctt aaagaactcc gaaagatccg tctgggcgac ccaggaggca
    18001 cacagcatca gcgtgtccgc tgcgttaccg ttggattcag cacagtagtt cttactgccg
    18061 aacttgttat tgccaacctc atcgccgcgc gctttacggt gcatcaactg gaacaggttc
    18121 cagcctttca tcccttcacg ctcgctgtaa aacttaggca ggtcaccttc tgggtaccac
    18181 tgtttgatat caaagttttt ctctgcccac tctttcagct gtgcgtacat cagcagacgg
    18241 tcccccgcac cgccgcgcgc ccaggcctga ccgttgctct cgtccagata ttccggcgcg
    18301 acggtaatgt cgtcagcgac acggttcatc ttgccgagat agcgatcctg catgtacagc
    18361 gccagcacgt tgttcgccac ttcagttgca cccggtacgt tcagcggtgt ttctgcagcg
    18421 ttatgaccgg cttcgtgcca aatcagccag tcgttcagcg gcgttgtcgg cagcgtggtg
    18481 ctgttcgtcg agaagctgct gttcattacc ggataacccg agtgcgcatc accgatggag
    18541 atctgcacat cgttggcgaa acggtgttta tgccccgtca atgcttcata ggtaaacatc
    18601 cggtggttac cgctcgtttc attgcgaccg tagaagtcat tcatcgagct ggcaaaggta
    18661 tccagatctt tagcgaattc tgctacacca ccagtgaaat tgctggcctc aaggttcttc
    18721 ttcggcgtgg tatagacgaa cgacgcagac tccagctcgc ccagcggcgc cggtgagtcc
    18781 agatcgtttt tccattcgcc gtctttatag aacggcgctt ttactacacc ggtaaaggtg
    18841 aagttagcag aaacatcatc cttactgtcg cccttgatat aaatcagacc accataaggc
    18901 accttgaagg tcacttcacc gttagcctcc agagtatacg ttttagtcac tcttggcgga
    18961 cggttcagcg caacttcatg cttctcacgt ccagtcaggt cgtcagccag cgccacggta
    19021 acagtcactg ggactgacgc cgaagactta atggtgacgt cctgctgggc cggtgcccac
    19081 aggccggttg actgcatgtt acccgcaaac catttggtcg gattcgagta caggctgatg
    19141 ttttccgtaa cgctctcacc ctttgccgat acggatcctg ggtacttctc cacatcaacc
    19201 ttaatgttca gatcccacca ggaacggccc agcatcagac gcgtcagcgg tttttccata
    19261 tagttgagcg gatagcttgg gttcatcatg cccgctttgc tgctaccgtc accgtagatc
    19321 atgttgttat cgaccagcga ttttttcaga tctgcggagc acttggtgcc gcctgcatag
    19381 gcatcattgg cgtagcagtt caggaactcg gtgaacgttt taaagcccag ctcatcttcc
    19441 ttgccctctt cgtaacgata tttcgtatcg ttccacagcc agaccgacat gttctggtac
    19501 agacgttcca gatcaacact gttcagacgc tcacctttac tgcctttggt acggaaataa
    19561 agctcatgct gatacaggcg ctgaatgttg gtgcctaaat ccgccgcctg caccatcgct
    19621 ttcgcggtgt cggcgtcaag attcagttgc gtatagcgcg gaacatacat gccacctgtt
    19681 accggaacat ccgtgcctgg gcggcgctcc aaacagttaa tctcgtaatg gtaagtcgag
    19741 tccttacact cctgtaaccc aggaaacttc tcaaagattt ttgcctttgc cgcttccaga
    19801 gattcttctg ttgtgtattc cgcttcatca ataaaggcat aacgcgttac ctgtttgccc
    19861 tcaacttcct cctgccagct cgcaacttcc agtttcggct tgtcatcagg cttgttgtct
    19921 tgctggtatt tccaggtcac ttcccctgta tttgggtcga tggtgtacgg cggttgcgcg
    19981 ccgtctgcag caggataacg ttcataaacc caaatgccag tcgcgcgacg ctgacgaacg
    20041 cgatccggat acccttgcgg atcgttgttc accaccgatt tgttcagagc cattgacaga
    20101 cccgcggcat ccagcagacg cacaaaactg gacgcgctct cttccttaag attgctcatc
    20161 acgttttcca tgatcagcac cgagccacct ttgttcagat aagcgatcag atcggtcaca
    20221 tcctgctgag tcagcttcgg tttgctggta tctgcacgca gaggcacagc atagggatcg
    20281 ccagaccact gagtcacata ttcaaagccg ttgaggatca gcaacggaat ctcttccgga
    20341 ttcagatcgc catagctggt caactgttta accgtgatac cagtgaaatc ctcatggaaa
    20401 gcaaatggtg cactatttcc caatacctgg cccgcttttt tgaaataaac gttctccagg
    20461 ttggtgccga cagtcatgat gctcttggta tttggctgcc agatgtcatt tgacaagtag
    20521 cgcaggacgt tctgcataaa gtgcttcatg tcatcagaat caccgctgag cgtacactca
    20581 cctttactat taacaccacc gccccaactg taaccgttcg ggcaacgcag gatgctgttg
    20641 tagtgcgggt taccgataac catcagtttg ccttcaccga cttgccccag cgaaataaac
    20701 ggcaggttga aagtcgcagt atcgcgcgta acgttctctg gctgcacaat ggaaggcgct
    20761 tccgtaatgt acgccagctc atttttatcc caggcgcgtt tttcgccaaa cgccagccag
    20821 tagtttttat cattacgcgc catcagaatc gggaatgccg agttggagat atttaccacc
    20881 gcctgaccgc gcgcgttacc ggtgctgcca tagaagttgg tagagtcatg gaagacgtgg
    20941 aacttgctga cagactgata gttcgtatcc acgccccaca gcttgttaat aacgccctga
    21001 atctggccgt cattaacatt gcgcgttgtc agcgagaacc agcgagcctc gttacaaccg
    21061 tcggttttcg cacaaatcgc ggtatcgatc tctttggcct gacccgtctt aaactgctcg
    21121 ataaattcgt taggcagcac aacgttttga tcgccttcat ccagcgtcgc accgttggat
    21181 aacgaaagat tgattatctc gttgatcacg ttgggatatt cggcaaagac cttgcgtaca
    21241 tagtccggaa caacacgagt attattttga ccagtcgtcg aataacgatg aatgagctga
    21301 tcgatatttg ccccgcgaac ttcatcaccc aattcagtca gcgcaatggt cgacttattg
    21361 ccacgtactg agcccagttc aaaggtatcg ataccaaagg agatgtttcg ccccagctaa
    21421 aggagaattt accgttttcg tcagttacgc cacggcctga attggtgtag tagtcaacgc
    21481 cagcaacacc gttccccagg ctatccacca gttggccttc ggaaagaatg atttcagtgg
    21541 gttgatactg ataaaactgt tccgcgttag ccgacacgaa ggacgcgttc agatccggtt
    21601 ttgttccctc tgtcgtgact ggcactaccg ttgaggtatg ggtggacggc gctttatcag
    21661 tcgcagcatt gttttccacc tcttcattga ccagcttgct gaaattgtct gttgccagat
    21721 caatttgctt atacagtttt tcaaatcgcg cgcgatcaac cactgacgag aaagtaagac
    21781 aaagctgttc tgcatctgcg gggcaactgt cgctggacgt caccagagag atggcgttgg
    21841 ttttcttatt ttcagaattc gccagctcct gcgcgtcctc caggctaaac gacactttgt
    21901 caaccgcacg caggctacgc gcagcttctg actgggtgtt gaatgttgca atggtcgtac
    21961 tgcccaccac acaactcacg gtattgcctg gcgtaaaggt aaagccatcg ctggattcac
    22021 cattacaggt agcaccagtt acccgctggc ttccgcccag ggtcagataa cccgttttcg
    22081 taggaacagg ttctggctcc ggttctggct ctggatcagg tgtcggttcc ggcgtaggtt
    22141 ctgggtccgg cgttggctca ggcgtcggct ccgggtttgg tgttggatca ggtttcactt
    22201 ccggcaaaga ccctgttcca gaatctacag gcggcgtatc ggaggaagat ccggaaccac
    22261 cgccatcaca accggctaac agggttgcgc tcaaaatagc cgctaaaagc
//
LOCUS       DAZGHF010000078        21377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGHF010000078 DAZGHF010000000
VERSION     DAZGHF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.078"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            2..859
                     /locus_tag="ACHK3A_003081"
     CDS             2..859
                     /locus_tag="ACHK3A_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO8741132.1"
                     /translation="MLIFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTRKL
                     ESIFNNTQVQIAVLIIPTTKDETIEQYATRVFNNWRLGDAKRNDGILIIVAWSDRTVR
                     IQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYSANP
                     SESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDLFLFS
                     DKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSGGGFSGGGGSS
                     GGGGASGRW"
     gene            complement(919..2217)
                     /gene="eno"
                     /locus_tag="ACHK3A_003082"
     CDS             complement(919..2217)
                     /gene="eno"
                     /locus_tag="ACHK3A_003082"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO8741133.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2305..3942)
                     /gene="pyrG"
                     /locus_tag="ACHK3A_003083"
     CDS             complement(2305..3942)
                     /gene="pyrG"
                     /locus_tag="ACHK3A_003083"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO8741134.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4170..4961)
                     /gene="mazG"
                     /locus_tag="ACHK3A_003084"
     CDS             complement(4170..4961)
                     /gene="mazG"
                     /locus_tag="ACHK3A_003084"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO8741135.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     GSAGNSREVLARWEQIKTEERAQKAQHSALDDIPSSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQVVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5057..7291)
                     /gene="relA"
                     /locus_tag="ACHK3A_003085"
     CDS             complement(5057..7291)
                     /gene="relA"
                     /locus_tag="ACHK3A_003085"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO8741136.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7339..8640)
                     /gene="rlmD"
                     /locus_tag="ACHK3A_003086"
     CDS             complement(7339..8640)
                     /gene="rlmD"
                     /locus_tag="ACHK3A_003086"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO8741137.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8697..11453
                     /gene="barA"
                     /locus_tag="ACHK3A_003087"
     CDS             8697..11453
                     /gene="barA"
                     /locus_tag="ACHK3A_003087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO8741138.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11677..13017)
                     /gene="gudD"
                     /locus_tag="ACHK3A_003088"
     CDS             complement(11677..13017)
                     /gene="gudD"
                     /locus_tag="ACHK3A_003088"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO8741139.1"
                     /translation="MSSQFSTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13038..14378)
                     /locus_tag="ACHK3A_003089"
     CDS             complement(13038..14378)
                     /locus_tag="ACHK3A_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO8741140.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVGLDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14380..15732)
                     /gene="gudP"
                     /locus_tag="ACHK3A_003090"
     CDS             complement(14380..15732)
                     /gene="gudP"
                     /locus_tag="ACHK3A_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO8741141.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16167..16616)
                     /locus_tag="ACHK3A_003091"
     CDS             complement(16167..16616)
                     /locus_tag="ACHK3A_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO8741142.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     SGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(16634..17416)
                     /gene="truC"
                     /locus_tag="ACHK3A_003092"
     CDS             complement(16634..17416)
                     /gene="truC"
                     /locus_tag="ACHK3A_003092"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO8741143.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDLIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMLVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17416..17745)
                     /locus_tag="ACHK3A_003093"
     CDS             complement(17416..17745)
                     /locus_tag="ACHK3A_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO8741144.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDKPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18367..18912)
                     /gene="syd"
                     /locus_tag="ACHK3A_003094"
     CDS             complement(18367..18912)
                     /gene="syd"
                     /locus_tag="ACHK3A_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO8741145.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18980..19828
                     /gene="queF"
                     /locus_tag="ACHK3A_003095"
     CDS             18980..19828
                     /gene="queF"
                     /locus_tag="ACHK3A_003095"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO8741146.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19940..21304
                     /gene="ppnN"
                     /locus_tag="ACHK3A_003096"
     CDS             19940..21304
                     /gene="ppnN"
                     /locus_tag="ACHK3A_003096"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO8741147.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5240 a         5710 c         5118 g         5309 t
ORIGIN      
        1 catactaata ttcacattcg tgtgttcctt tgttgcagcc caaccaacta ttgtcccaca
       61 attacagcaa caagttacgg atcttacgag tagcttaaac tcacaagaaa agaaagaact
      121 gacgcggaag ttagaatcta ttttcaataa tacccaagta caaattgctg tattaattat
      181 tcccacaacc aaagacgaga caattgaaca atatgctaca agggttttta acaattggcg
      241 tttaggagat gccaaacgca atgatgggat actgatcatt gttgcctggt cggatcgcac
      301 tgtccgcatc caggtaggtt atgggctgga agaaaaggta accgatgctc tggctggaga
      361 tatcatccgt agcaacatga tacccgcatt taaacaacaa aaattagcta agggattaga
      421 gttagctata atcgctttga ataaccaact cacttcacaa caccaatatt cggctaatcc
      481 ttcagaaagt gaatcagcgt cttccagcga tcattactac tttgctattt tttgggtatt
      541 tgcagtgatg ttcttccctt tctggttttt tcatcaaggc agtaattttt gtcgcgcatg
      601 taaaagcagt gtctgtattt cagcaatcta tcttttagat ttattcctgt tctccgataa
      661 aactttttcc attgctgtat tttccttctt ttttactttc accatattta tggtctttac
      721 ctgtttatgt gtacttcaga aaagagcatc aggtagaagt tatcattcag acaacagcgg
      781 ttccgctgga ggctcaggcg gcgggtttag cggcggtggc ggttcttctg gcggtggcgg
      841 cgcatccggc cgctggtaac tcaaccataa aaaacgccag cccgaaggct ggcattttta
      901 aatcagataa agtcaggctt atgcctggcc tttgatctct ttacgaccgt tgtacggtgc
      961 tttttcgccc agagcttctt cgatacgaat cagctggttg tatttagcaa cacggtcaga
     1021 acggctcata gaaccagttt tgatctggcc tgcagcagta ccaacagcca ggtcagcgat
     1081 agtagcgtct tcagtttcgc cagaacggtg agagataact gcagtgtagc cagcgtcttt
     1141 cgccatcttg attgcagcca gagtttcggt cagagaaccg atctggttga atttgatcag
     1201 gatggagtta gcgatacctt tttcgatacc ttctttcagg atcttggtgt tggttacgaa
     1261 caggtcgtca ccaaccagct ggattttgtc gcccagaact ttggtctggt atgcgaaacc
     1321 gtcccagtca gattcgtcca gaccgtcttc gatagatacg atcggatact gtttggtcag
     1381 ttcttccagg aagtgagtga attcttcaga ggtgaacgct ttgttgcctt cgccagccag
     1441 aacgtattta ccgtctttgt agaattcaga agctgcgcag tccatcgcca gagtgatgtc
     1501 tttgcccagt tcataaccag cagctttaac agcttcagcg ataacagcca gagcttcagc
     1561 gttggaaccc aggttcggcg catagccacc ttcgtcacca actgcagtgt tcatgccttt
     1621 cgctttcaga acttttgcca ggtgatggaa aacttcagaa cccatgcgga tggcttcttt
     1681 cacagttttc gcgccaaccg gctgaatcat gaattcctgg atatcaacgt tgttgtcagc
     1741 gtgctcacca ccgttgatga tgttcatcat cggaaccggc atggagtatt tgcccggagt
     1801 gccgttcagt tcagcgatgt gctcgtacag cggcatacct ttagcagctg cagcagcttt
     1861 ggcgttagcc agagatacag ccaggattgc gttcgcgccg aatttggatt tgttttcggt
     1921 gccgtccagg tcgatcatga tcttgtcaat gccagcctga tctttagcat ctttgccaat
     1981 cagcgcctga gcgatcgggc cgtttaccgc agcaacagct ttggttacgc ctttacccag
     2041 gaaacgggat ttgtcgccat cgcgcagttc cagagcttca cgggaaccag tagaagcacc
     2101 tgacggagca gctgccatac cgacgaaacc accctccaga tgtacttcgg cttcaacagt
     2161 cgggttacca cgggagtcga tgatttcacg accgatgatt tttacgattt tggacattag
     2221 gttttcctca agtcactagt taaactgaaa ctccagacaa acaacgcgta cccagggtac
     2281 gcgttgccgt tctaactttt ttacttactt cgcctgacgt ttctggaact cgctggcggc
     2341 tttcacaaag cctgcaaaca gcgggtgacc atcacgtgga gtagaagtaa actccggatg
     2401 gaactggcaa gccacgaacc acgggtgatt cggaacttcg atgatctcga ccaactgatc
     2461 atccccggaa cggcccgcaa cgcgcagacc tgcatcttca atctgtttca acagcatgtt
     2521 gttgacttcg taacggtgac gatgacgctc aacaattgtc ggcgcattgt acagctggcg
     2581 aaccaggcta tcgtcaacca actggcactg ctgtgcgccg agacgcatgg taccgccgag
     2641 atcgctcttc tcgctacgaa cttcaacgtt gccgttttca tcgcgccact cggtaatcag
     2701 cgccacaact gggtacttac agtctggcac aaattccgta gagttggcgt tctccatgtt
     2761 ggcaacatgg cgagcgtaat caattaacgc cacctgcata cccaggcaaa tacccagata
     2821 aggaatattg ttctcacgcg caaaacgcgc ggtcgtaatc atgccttcta cgccacgata
     2881 gccgaaaccg ccaggtacga ggattgcgtc cagaccttta aggatttcaa cgccgcgcgt
     2941 ttcaacatct tgtgaatcga tcagtttgat gttgacgctg acacgattct tcagcccacc
     3001 gtgtttcagt gcttcgatca ctgatttata agcatccggc agttcaatgt acttgccgac
     3061 cataccgatg gtgacttcac ttaccggatt cgcttcttcg aagataacct gttcccattc
     3121 ggacagattt gcttccgggc agtttaagct gaatcgttta caaatataat cgtccagccc
     3181 ctgagatttc aacaggcccg gaattttata gatggaatcg acgtctttca gagaaataac
     3241 cgctttttcc ggaacattac agaacaatgc aatcttcgca cgttcgttcg ccggaacggc
     3301 gcgatctgaa cgacaaatca ggatgtcagg ctggataccg atggagagca gctcttttac
     3361 agagtgctga gtcggtttgg ttttgacttc accagacgct gccatgtacg gcaccagcgt
     3421 caggtgcata aacagagtgt gctcacggcc aatttcaaca gccatctggc gaatcgcttc
     3481 gaggaacggc agggattcga tatcacctac agtaccgccg atttcgacca gtactacgtc
     3541 atgaccttcg ccgccttcca gcacgcgctc tttgattgcg ttagtgatgt gcggaataac
     3601 ctgcacggtt gcgccgaggt agtcaccgcg gcgttcttta cgcagaacgt cagagtagat
     3661 acgacccgtg gtgaagttgt tgcggcggct cattttggta cgaatgaaac gctcgtagtg
     3721 ccccaggtcc aggtcggttt cagcgccgtc ttcagtaacg aacacttccc cgtgttggat
     3781 tgggctcata gtacctggat cgacgttgat gtacggatcc agtttcatga tggtcacatt
     3841 gaggccacgg gcttcaagaa tggctgcgag ggaggctgcg gcaatgcctt tacccagaga
     3901 ggatacgacc ccgccggtca caaaaatata gttcgttgtc atgctgaacc tgagaagtta
     3961 ggttgaaaag acgatggaat aaccaggacg ggaaagcagt atacccgaac atgacctgtg
     4021 ccacaaactt tcattatccc tcctcttcgc cagcgcacta ttgaaatcag gagtgagaaa
     4081 atagcccctt tggggtaaat gtttttgacg caaatcaagc gcttgtcatt taaaaaatga
     4141 cacaaatcgc gcttgaccac gtaattccct tagagatcaa tttcctgccg ttttacctgt
     4201 tgccagactt cttccattgt ttcgaggtca acacctgtca tttccagtcc acgcgcggca
     4261 acaatacgct ccacttcgcg aaaacgacgc tcgaattttt cgttcgcttt ttgcaatgcg
     4321 atttccgctt ttgtccctaa atgacgagcc agattgactg tggcaaacag caggtcaccc
     4381 atttcctcct ccagtttagc ctggtcgaca acaacctgcc gcgcttcgta catcacctca
     4441 tcgatctctt cgtagacttt atcgactacc gggccaagcg tcgtccagtc gaagccaacg
     4501 ttggcgcaac gtttctggat tttttgtgcg cgcattaagg cgggtaaact actagggata
     4561 tcgtccagcg ccgaatgctg cgctttttgc gcgcgctctt cagttttgat ttgctcccaa
     4621 cgagcaagaa cttcgcggct gtttccggca gaaccgtcag caaaaacatg tggatgacgg
     4681 cgttctaatt tatcgctaat cgcagcgcaa atatcattaa aatcaaagcg cccttcttcc
     4741 tgggccattt gtgcgtaaaa caccacctgg aacagcagat cgcccagttc accgcgcaga
     4801 tcgtcaaaat cttcacgggc gatggcgtcc agcacctcgt aggtttcttc aagggtgtaa
     4861 ggcgcaatgg tggcaaatgt ctgctcttta tcccacgggc agccgttttc cgggtcacgc
     4921 aggcgctgca tgatggtgag caaacggtcg atttgattca ttagaatgtc ctgaaaattg
     4981 cgggtccgtc aggtgtaata ccttcgagta aaagatatcg ctggatacgg ctgacgccat
     5041 atccagctat gtaagactaa ctcccgtgca accgacgcgc gtcgataaca tccggcacct
     5101 ggttgagttt acccagcacg cgccccagca cttgcaggtt gtaaatctca atggtcatgt
     5161 cgatggtcgc cagttgctgt ttggtgtcgc tacggctggc aacgccaagt acgttcacct
     5221 tctcgttggc gagaatggtc gtgatatcac gtaacaaccc actacgatca ttagccacca
     5281 cgcggaccac cagcgaatat ccggcggagt agctctcgcc ccataccgcg tcaacaatgc
     5341 gttctggcgc atgggagcgc agttccgcca attgttcgca atcggcgcgg tgtactgaaa
     5401 taccgcgccc ctgggtaatg aagccgacaa tctcatctcc aggaatcggc tggcagcagc
     5461 gcgcgatgtg gtgcatcagg ttaccgaccc cttctaccac cacacgaccg ttatctttac
     5521 tgcggttttg cggcgtgtag cttttttgct gaagctgttt cagcgcggcg gcgtcctgct
     5581 cttcggcact cggcttatta aattgcgatt gcaggaagtt caccatctga ttgagacgga
     5641 tatccccgcc accaatcgcc gccagcaact cgtcgacatc attgaagttg taacgcggca
     5701 gcagatgttt ttctgcttct ttcaggctga tccccagatg ttccagctcg tcgtcaagaa
     5761 tttgccgccc agccagaatg tttttgtcac ggtcctgttt acggaaccag gcgtgaattt
     5821 tcgaacgccc acggctggtt gtgacgtaac cgaggtttgg gtttaaccag tcacggctgg
     5881 ggttcggctg tttctgggtg ataatttcaa tctggtcgcc catctgcagc tggtaggtga
     5941 acggcacaat gcgcccgcca atttttgccc cgatgcagcg gtgtccgaca tcactgtgga
     6001 tgtggtaagc gaagtccagc ggcgttgatc ccgcaggcaa atcaacgaca tcacctttcg
     6061 gcgtaaagac gtacactcgg tcgtcaaaga cctgactacg tacttcgtcg agcatttcgc
     6121 cggaatcagc catctcttcc tgccacgcaa tcagtttacg cagccaggca atccggtctt
     6181 catgtcccga acgtgcgccg ccagcagccg cgccctcttt atatttccag tgcgcagcaa
     6241 cacccaactc tgcatcttca tgcatctgtt tggtgcggat ttggatctca acggttttgc
     6301 cacccggccc cagaaccacg gtatgaatgg actgataacc gtttggtttc gggttagcga
     6361 cgtaatcgtc aaactcatcc ggcaggtggc gatagtgagt gtgcactatc cccagtgcgg
     6421 cataacaatc ctgtaaacgc tcggcgacaa tacgtaccgc acgcacatca aacagctcat
     6481 cgaaggcgag gttctttttc tgcattttgc gccagatact gtagatgtgt ttcggacggc
     6541 catacacttc agctttaacg ccttcagctt tcatctcagc gcgcagatgg ccgacaaact
     6601 cttcgatata gtgttcgcgg tcgagacggc gttcatgcag cagttttgcg atgcgtttgt
     6661 attcggtcgg gtggagataa cggaagcagt aatcttccag ctcccatttc agttgcccaa
     6721 tcccaagacg gttcgccaac ggcgcgtaga tattggtgca ctcttttgcc gccagtacgc
     6781 gttcatcttc cggcgcatct tttacttcgc gcaggtgagc aatacgctcc gccagtttga
     6841 tgaccacgca gcgaaaatca tcgaccatcg ccaataacat ccggcgaacg ttatcgacct
     6901 gttcggagga aacagaatca gtgtgcgtcg ctttcagctg gcggatcgcc gccatatcac
     6961 gcacgccgtg aataaggtta acgaccgact taccgacgct ctcacgcagc acatcttcgc
     7021 tgactacgtt ggcatcagcc agagggaaca gcagcgccgc ccgcagcgtg tcaatgtcca
     7081 tacttaatgt cgagaggatc tccaccatct caacaccacg ccacaataac agactggcat
     7141 ccggatgccc ctgcgtctgt tgcagacaat acgcccaggt ttcggctaag cactcacacg
     7201 acttctggct ggtaataccc agacttgcga tccatttttc cggatcaaat tcaccagcct
     7261 tattgatatg tgcacttctt accgcaacca tcgtcctctc ctttagggac cagacctgcc
     7321 gaaatcggca aatcgtaact atttaacgcg cgagaaaagt accatcgatt ccagatgtcc
     7381 cgtgtgtggg aacatatcca gcatcgccag tcgcgcaatg gtatatcctg cttttaataa
     7441 cgcttcgcta tcccgagcca gcgttgcagg gttacaggat acataaacta tacgaatagg
     7501 ttccagtttt ataatttgct gcataacacc tgcggcacct gctcgcgccg ggtccaacaa
     7561 cactttatcg aagccgtttt tcgcccacgg ctgctttgtg acatcttctt caagattttc
     7621 gtgataaaac gtcacattct gtaagccatt aagacgcgca ttctgctggc ctttttccac
     7681 cagcgccgga acaccttcta cacccaccac actagcagct tgtgtcgcca atggcagtgt
     7741 aaagttgccc ataccgcaga acagatccag tacgcgatct tcaggttcca catccagcca
     7801 ttccaacgca cgcgctacca ttttctggtt cacacccgca ttgacctgaa taaaatcgcg
     7861 cgggctaaaa gttaagcgca acccatttga gtcataccag ggcatctcac cagagacggt
     7921 ttcgagtatc tcactatcgg gggcgagata cagatccagg ccttcagaat gcgaaaagcg
     7981 ttccagtttt tcgcgatcag ccgaacttag cggtgcggta tggcgcaaaa tcatcagcgt
     8041 gccgctggtt gcctgtacca gttcaacatg accaagatgg cgtatagctt gtaagctgcc
     8101 caggcatgcc ctgactttgg gcagcaatgc ttcaagttgg ggcactaaaa tgggacattg
     8161 tttaacgtcg acaatgtcac tggagcccgc tttgcgaaac cccatctgaa gttgctgtgt
     8221 tttcggtaag tagttcagac ttaaacgcgc gcgacggcga tagccccagg gaacatcggc
     8281 gatcacttca gagacttcgt gtttcattaa tcgggcgagt gccgcacttt tgcttcgctg
     8341 ctgtaaatcc acgctggcgt gttgttgctg acagccgccg catacgccaa aatgaggaca
     8401 gcgtggcgtt tcgcgttccg ggctatcgct taaccggcgc acgactttag cgcgggcgta
     8461 ttgtttttta tcttcagtaa cagtaacttc cgcgttttcc tgtggcaata atccaggaat
     8521 aaataacgct ttgccgttat gtcgtgccac gccctgacca aaagagtcga ggtcgttgac
     8581 tgaaacagtt atgatctgac gcgtcgtcgt gcgtcgtttt gcagagtaga attgcgccat
     8641 tggcgagact ttctcaattt aacagtgtga ccttaattgt cccataacgg aactccatga
     8701 ccaactacag cctgcgcgca cgcatgatga ttctgatcct ggcaccgacc gtccttattg
     8761 gtttattgct gagtatcttt ttcgttgtgc atcgctataa cgacttgcag cgtcaactgg
     8821 aagatgccgg agccagcatt attgagccgc ttgcagtttc tactgaatat ggcatgagcc
     8881 tgcaaaatcg cgaatctatc ggtcagttaa taagcgtact gcatcgtcgc cattccgata
     8941 ttgttcgcgc gatttcggtt tatgatgaaa ataaccgact ctttgtcacc tccaattttc
     9001 atcttgatcc ctcatcaatg cagctcggca gcaacgtgcc gtttcctcgc cagctcactg
     9061 tcactcgtga cggcgatatt atgatcctcc gcacgccgat tatttctgaa agttactccc
     9121 ccgacgaatc gcccagtagc gatgccaaaa atagtcaaaa tatgctggga tatattgcgc
     9181 tggagctgga tcttaaatcg gttcgcttgc agcaatataa agagatcttt atttccagcg
     9241 tgatgatgct gttttgtatc ggtattgcgc ttatttttgg ctggcgctta atgcgcgatg
     9301 taaccggtcc gattcgcaac atggtgaata ccgtcgaccg catccgtcgc gggcaactcg
     9361 acagccgggt ggaaggattt atgctcggcg agctggatat gctgaaaaac ggtatcaact
     9421 cgatggcaat gtcgctggcg gcttatcacg aagagatgca acacaatatc gaccaggcga
     9481 cgtccgatct gcgtgagacg ctggagcaaa tggagattca gaacgttgag ttggatctgg
     9541 cgaaaaagcg cgcccaggaa gcggcgcgta ttaaatccga gtttctggca aatatgtcac
     9601 acgagctgcg tacaccactg aatggtgtta ttggctttac ccgcctgacg ctgaaaacag
     9661 aattaacacc aacgcagcgc gatcacctga atacgattga acgttcggca aataatttgc
     9721 tggcaattat taatgatgtt ctcgacttct cgaaactgga agccggtaag ctgattctgg
     9781 aaagtattcc attcccacta cgcagcacgc tggatgaagt cgttaccctg ctggcacatt
     9841 cttctcacga taaagggctg gaactgacgc tcaatattaa aagcgacgtg cctgataacg
     9901 tgatcggcga cccactacga ttacagcaaa tcatcactaa cctggtaggg aatgcaatta
     9961 aattcaccga gaatggcaac atcgatattc tggtagaaaa acgtgcgctg agtaatacca
    10021 aagtgcagat tgaagtgcag attcgggata ccggcattgg tattcctgaa cgcgatcaat
    10081 cgcgcttatt ccaggccttc agacaggctg atgccagtat ttcccgccgt catggtggca
    10141 ccgggctggg gctggtgatt acacaaaaac tggttaatga aatgggcggc gatatttcgt
    10201 tccatagcct gccgaatcgt ggttcaactt tctggttcca cattaatctc gatctgaacc
    10261 cgaacattat tatcgaaggg ccatccaccc agtgcctcgc tggtaagcgc ctggcctatg
    10321 tcgaaccaaa ctccgcagca gcacaatgca cgctggatat tttaagtgaa acgccgctgg
    10381 aagtggttta tagcccaacg ttctccgcgc tgcctcccgc gcattacgac atgatgttat
    10441 taggcatagc ggtgaccttc cgcgagccgc taacaatgca acatgagcga ttagcgaaag
    10501 cagtatcgat gaccgatttc ctgatgctgg cacttccttg ccatgcacaa gtcaatgctg
    10561 aaaaacttaa gcaagatggt atcggcgcgt gtctgctgaa accattaaca cctacgcgcc
    10621 tgttgcctgc cctgacggaa ttttgtcatc acaaacaaaa cacgcttttg cctgtaaccg
    10681 atgaaagtaa gctggcaatg acagtcatgg cggttgatga caaccccgct aacctgaaac
    10741 ttatcggcgc attgctggaa gatatggtgc aacatgtgga actttgcgat agcgggcatc
    10801 aggcggttga acgggcgaaa cagatgccgt tcgatttaat cttaatggat attcaaatgc
    10861 cggacatgga cggcattcgg gcatgtgagc tcatccacca gctcccgcat cagcagcaaa
    10921 cgccggttat cgcggtaacg gcgcatgcga tggccgggca aaaagagaaa ctgcttggcg
    10981 cggggatgag tgattatctg gccaaaccga tcgaagaaga gcgattgcat aatttgctgt
    11041 tgcgctacaa gcctggcagc ggtatttcct ctcgcgtcgt gacgcccgaa gtcaacgaaa
    11101 ttgtggtcaa cccgaatgcg accctcgact ggcaactggc actacgccag gcagcaggaa
    11161 aaaccgattt agcgcgcgat atgctgcaaa tgttactcga tttcctgcct gaagttcgca
    11221 acaaagttga ggaacagctg gttggagaaa acccggaagg cctggttgat ttgattcata
    11281 aactgcatgg cagttgcggc tatagcggtg taccgcgtat gaagaatctc tgccaactga
    11341 tcgaacaaca gctacgtagc ggtactaaag aagaagattt agagccggag ctgctggaac
    11401 tgttggacga gatggataat gtagcgcgcg aagccagcaa aattctcggg taatggacgt
    11461 cttgtcgagt agcaaccgga tttttatgac gctggtttca gattggtgaa aagtgcctgt
    11521 gtttatgccg gatgcggcgt taacgcctta tccggcctac aaaaccaatt aaattcaatg
    11581 aattaaaaaa tgatgtaacc ctgatacgta gcgcaacagg cagtttggca tcagtctcaa
    11641 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11701 gttatcaaac gtccagccgg ggatcaggta ctgcattccc atcgcatcgt ctcgcgcacc
    11761 aagcccatgt ttctgataca gctcgtgggc tttcatcacc tgatccatat cgatttctac
    11821 gcccaatcct ggtttttgcg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11881 ggtcagacgc tgattgcctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11941 cggtgcagca gcggctacat gagtaaacat cgccagggaa atatcgaagt ggttgttaga
    12001 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12061 catcgtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12121 gcccatctgc cgccagtcgg tggcgatcat attggttgcg gtcggcaggc cagtcgcgcg
    12181 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12241 tgcataagcc agcgaacctt tcaggtattt accaatttta atcgcttcgt tcagcgacca
    12301 ggcaccgtta ggatcgaggg taatacgcgc ctgtgggaag cgttgcgcca gtgccacaat
    12361 agattctgct tcttcttccc cggccagtac accgcccttc agtttgaaat cgttgaagcc
    12421 atatttttca tatgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12481 gtgacgcagg cgataccagt cacatttatc atccggctgg ctttgatacg gcagcggtgt
    12541 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12601 accatcgccc agcagcgatg ccacgttaac ccccagatgc tgccccagca gatccagcat
    12661 tgccgcttct atcccggtaa ccacatgaat agtggtccgc aggtcaaatg tctgcaaacc
    12721 gcgcccgccg gcatcacgat cggcaaatgt attacgcacc agcgtcagaa cgtttttgta
    12781 ttcacccagc gttttaccca ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12841 ctcgccgccg ggaatttccc ctaccccggt gtgaccagaa ttatctttga taatcacaat
    12901 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatggcc
    12961 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gaaaattgag aactcatctt
    13021 tatgtccttt ttcaaaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13081 ccggggatca ggtactgcat cgggcccgcg tcgttacgcg cacccccagg cagacgttta
    13141 taggcctcat gcgccttttg tacctgctcc cagtccagtc ccacgcccag accgggcgca
    13201 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13261 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcaccc
    13321 acatgggtaa acatcgccag agagatatca aagtggttat tggaatggca gccccaggtc
    13381 agcccccagt cgtcgcaaag ttgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13441 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13501 ttggtggcga tcatgttggt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13561 acttcacgac cggagaaacc ctgctcagcg ccgcacgggt cttccgcata ggtaaggaca
    13621 tcattcagcc ctttgcacag agaaatggct tcatcaagca gccaggcacc attgggatca
    13681 acggtaatcc gcgcatcagg gaagcgtttc ttcaatgcac gaacagtgtc gatttcttgc
    13741 tcgccaggta acacgccacc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13801 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatgccg caactgatac
    13861 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg
    13921 atataaaaca gataaccgag aacggtaata gcatcacgtt gcttgcctgg ccctaacagt
    13981 tcgcagaccg gaacattcag cgcctgacct aacaggtcaa gcaaggcggc ttccagcgcc
    14041 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    14101 gctgcctgat tacctttatg cacctgctga accactttat tcaggcgcgc aacttcctgg
    14161 cccagcacca tcggaatagc cttgaccagt gtttgataaa tcacctctcc gcccggcgct
    14221 tcaccaatgc cggtatgccc ggcattatcg gtgagtacca caatattgcg ggtgaaatat
    14281 gcgttatgtg caccaccaat attaagcaac atgctgtcat gtccggcgac cggaatgact
    14341 ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt
    14401 cagctcaata cgcttgatat cgcccaccag caccaggtag ctgagcaccg cgattaaggc
    14461 atgaacacca acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc
    14521 aattgcgatt ggcgtgacga tgccagaaat gttaccaaac atgttaaaca ggccaccgga
    14581 aagaccgctg atctcttttg gcgcagtatc agccattaca gcccaaccca gcgcaccgat
    14641 ccccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14701 gttgcagaac accatcacca tcgacaacaa catgcccatc acgatcggtg ttttacgcgc
    14761 aatgttcagt gaacccgtgc gacgcatcag ccagtcggaa ataatcccac ccagcacacc
    14821 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14881 cccgcgcgcc tgcaccagat aaaccgggaa ccaggtaatg aaaaagtaag tcagggcgtt
    14941 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    15001 ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat ccatattgat
    15061 cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacgcccg gatgttgatt
    15121 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15181 gaagaagacg tgtgaccagc ccacttcatg agttagccag cccataatcg gcgcgaagat
    15241 aaccgttgcg aagtattggg cggagttaaa aatcgacacc gccgttcccc tttcctgcgc
    15301 cggaaaccag gccgcaacaa tgcggctgtt gccagggaaa gatggcgctt cagcaagccc
    15361 gaccaggaag cgcagtgtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15421 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15481 tgaaccaaaa cgatccagca accagccacc agggatctgc ccgataacat acgcccatga
    15541 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15601 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15661 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15721 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15781 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15841 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15901 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15961 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gttaaaacga
    16021 agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc
    16081 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16141 acggcattta ccgctcgctg aacatatcag gacaacatcg tgccccaatg ttcgacccac
    16201 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16261 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccact attgcagaaa
    16321 ttcacataac tactgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16381 agggtatctt taattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16441 gtcgtggacg taaccaccag aacatactta tcctgatagg gcagccagtc gcttaattca
    16501 ggatcttcaa ataccgttgc tttgtggccc tgcgcggtca gaattgcttc cgcttcttcc
    16561 gccactaaca gcgaatttcc gtacatagtg ccgacaaaaa taccaatttc cgccataccg
    16621 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16681 ccctttcatt ttcaggaagc agaccccgcc agccaaattg tgataacgcc tgcatccagg
    16741 tgtcgtccag gcccgcgtga atagtcagtg gctcgccagt aaaaggatgc gtcaatgaca
    16801 actgactggc atgcagcatt aaccgctgaa ggccaaaatg ttcagcaccg ctgcgattct
    16861 ggcgtaaatc accatgtttg ctgtcgccaa taatcgggtg gcgcaaatgg gcaagatggc
    16921 gacggagctg gtgcttacgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16981 tcgggtaacg tccggtcgct accagcattt ctacggtcgc cagaccgcga taatgcgtca
    17041 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    17101 ccaccagtgg ataatccagc accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    17161 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    17221 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaacacat
    17281 gctggcctat caggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17341 agctgcggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17401 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccagctc
    17461 agccagaata agcgcgcgtt gcggatgatc agtcgccagc gccatttcat aatagggtgc
    17521 aacggcaaaa gcgcccggta acggctggtt gttatctaac aaatcgtgca ttcgcgggat
    17581 cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17641 gttaaattga tgcggctggg gtttatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17701 ttcaagcgcc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacctcaata
    17761 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17821 cccggttcgt ttcgcagcat tccggctact tttgttgctc cctgctcatc cttgacaact
    17881 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17941 ccgatgtgat acttcatcct gaagtgttcc tggccttcct gacccaccga atcatcctga
    18001 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    18061 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tccttcagaa gtgtcatcat
    18121 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    18181 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18241 ttaacaagca acatgttgtt tttaaatgag aataacgatt cttatctttt taagtattcg
    18301 cttatttcct ggcgatctcc tacagagtct gtaagagatc tctcacaacc agatggggta
    18361 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18421 cgtccgttta cgtgttccta gcgtctcttt gcacacttca ccggagagat tacacaccga
    18481 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18541 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaaat cgtcttcact
    18601 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18661 ctgagtcgta taaaaggtat gaatcgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18721 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18781 agttgaaata atgcacggtg aaggaacgcc gtacagttcc tcacttaacg gccagctttt
    18841 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18901 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18961 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    19021 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    19081 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    19141 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    19201 tgtcggtcat gttgaacttg attacaccag cgcaaatctg attgagtcga agagttttaa
    19261 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19321 gctggagcgc gacttaagca cttgcgctca gggtaaggtt agcgtggcgt tatatcgtct
    19381 tgatgaactg gaaggccaac cgataggtca tttcaatggc acttgcattg atgaccagga
    19441 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca ccagtggcga
    19501 aaaagtagta gaagagacgc tcgtcagcca cctgctgaaa tcaaattgcc tgatcaccca
    19561 tcaaccagat tggggatcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19621 actgctgcgt tacctggttt ctttccgtca tcacaacgag ttccacgaac agtgcgtgga
    19681 acgcatcttt aatgacctgt tacgcttctg ccagccagag aaattgagcg tttacgcacg
    19741 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19801 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19861 cagaaggttg tgaaaggtca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19921 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19981 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    20041 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    20101 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    20161 ggaactgata aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgctttgca
    20221 ggccaacttg ttcgcggttc tgcgtgacat tctcttcgtt tacgggcaaa tccataacac
    20281 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20341 catcttgcgt aacgctcgcg cactgcatgt cggtgaagcg ccaaatatgg tggtctgctg
    20401 gggcgggcac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaatcagct
    20461 gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat
    20521 gaaaggcgct gcggtcggac acgcacagca gcgttacaaa gacagtcgtt ttattggtat
    20581 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20641 catgccggat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gtatcattat
    20701 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20761 cccggccaac aaagatcagg ttttaccatt gatcctcacc ggcccgaaag agagcgccga
    20821 ctacttccgc gtactggacg agtttgtcgt gcatacattg ggtgaaaacg cgcgccgcca
    20881 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20941 gctggtgaaa gaaaatcgcc gcgacacagg cgatgcctac agctttaact ggtcaatgcg
    21001 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    21061 gctttacccg gatcaacccg ttgaagtgct ggctgccgac ctgcgccgtg cgttctccgg
    21121 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    21181 taaaatcaac ggcgataaag agattatgcg tcgtatggat gacctgctac agggttttgt
    21241 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21301 gtaatctccc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg tcacataccc
    21361 atcaatttat ttcattt
//
LOCUS       DAZGHF010000079        21292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGHF010000079 DAZGHF010000000
VERSION     DAZGHF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.079"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            131..1453
                     /locus_tag="ACHK3A_003097"
     CDS             131..1453
                     /locus_tag="ACHK3A_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8741148.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(1632..3692)
                     /locus_tag="ACHK3A_003098"
     CDS             complement(1632..3692)
                     /locus_tag="ACHK3A_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO8741149.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLASDDNKDTNAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(3762..4529)
                     /gene="pdeH"
                     /locus_tag="ACHK3A_003099"
     CDS             complement(3762..4529)
                     /gene="pdeH"
                     /locus_tag="ACHK3A_003099"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO8741150.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            4761..5690
                     /gene="kdgK"
                     /locus_tag="ACHK3A_003100"
     CDS             4761..5690
                     /gene="kdgK"
                     /locus_tag="ACHK3A_003100"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO8741151.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSQTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(5786..7282)
                     /locus_tag="ACHK3A_003101"
     CDS             complement(5786..7282)
                     /locus_tag="ACHK3A_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO8741152.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(7503..8789)
                     /gene="dctA"
                     /locus_tag="ACHK3A_003102"
     CDS             complement(7503..8789)
                     /gene="dctA"
                     /locus_tag="ACHK3A_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO8741153.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(8972..10960)
                     /gene="hmsP"
                     /locus_tag="ACHK3A_003103"
     CDS             complement(8972..10960)
                     /gene="hmsP"
                     /locus_tag="ACHK3A_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO8741154.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(11042..14533)
                     /gene="bcsC"
                     /locus_tag="ACHK3A_003104"
     CDS             complement(11042..14533)
                     /gene="bcsC"
                     /locus_tag="ACHK3A_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO8741155.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQAGQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQW
                     SSNIQELVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRD
                     YTAARAAYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRR
                     VALAQAQLGDTAAAQQTFNKLIPLAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETY
                     KDAMVASGVTTTRPLDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDY
                     WGSSGTGGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGT
                     CTLQDCSGNRSQSDSGASVAVGWRNDTWSWDIGTTPMGFNVVDVVGGISYSDDIGPLG
                     YTVNAHRRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLS
                     GDQLTGKNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGY
                     YSPQEYLSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAA
                     RQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGW
                     QGDMDLPPQPLIPYADW"
     gene            complement(14515..15621)
                     /gene="bcsZ"
                     /locus_tag="ACHK3A_003105"
     CDS             complement(14515..15621)
                     /gene="bcsZ"
                     /locus_tag="ACHK3A_003105"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO8741156.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWALLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(15628..17967)
                     /gene="bcsB"
                     /locus_tag="ACHK3A_003106"
     CDS             complement(15628..17967)
                     /gene="bcsB"
                     /locus_tag="ACHK3A_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO8741157.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(17978..20596)
                     /gene="bcsA"
                     /locus_tag="ACHK3A_003107"
     CDS             complement(17978..20596)
                     /gene="bcsA"
                     /locus_tag="ACHK3A_003107"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO8741158.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(20593..>21292)
                     /gene="bcsQ"
                     /locus_tag="ACHK3A_003108"
     CDS             complement(20593..>21292)
                     /gene="bcsQ"
                     /locus_tag="ACHK3A_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO8741159.1"
                     /translation="TAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWARAM
                     LDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICTGLQQLKASGRYQW
                     ILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIGSQ
                     VQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANWCL
                     LNYSGLKTPVGSAS"
BASE COUNT         4873 a         6010 c         5484 g         4925 t
ORIGIN      
        1 aaatcactgg cctaaacatt atcccctttt tgcctggttt ttgaccattt ccgcgatttg
       61 ttacacattg aaatatcact tttgctgtgc gtaatatggc tattcgttag ccaaaaaata
      121 agaaaagatt atgcaagcaa cagccacaac actcgaccac gagcaagaat acacgccgat
      181 caactcgcgt aataaagtcc ttgtcgcctc tctcattggc acggccattg agttcttcga
      241 cttttacatt tacgccactg cggccgttat tgtgtttccg catatcttct tcccgcaggg
      301 cgatcctacg gcagcaacgc tacagtcgct cgccaccttt gccatcgcct tcgtcgcgcg
      361 ccccattggc tctgccgttt ttggtcattt tggcgatcgc gttgggcgta aagcgacgct
      421 ggtcgcctcg ttgctaacga tggggatttc gaccgtggtg attggcctgc tgccaggcta
      481 tgccacgatt ggtattttcg ccccgctgct gctggcgctg gctcgatttg gtcagggtct
      541 gggcttaggt ggtgaatggg gcggcgcggc tctgctggcg actgaaaacg ccccaccgcg
      601 caaacgtgca ttgtacggct ccttcccgca gctgggcgca ccgattggct tcttctttgc
      661 caacggcact ttcttgctgc tttcctggct actgaccgac gagcagttta tgagctgggg
      721 ctggcgtgtg ccatttatct tctcggcggt gctggtcatt atcggcctgt atgttcgcgt
      781 gtcgctgcat gagtcgccgg tgtttgagaa agtcgctaaa gcgaaaaaac aggtgaagat
      841 cccgctgggt acgctgctga ccaaacatgt tcgcgtaacc gtactgggta cgttcattat
      901 gctggcaacc tatacgctgt tttacatcat gacggtctac tctatgacct ttagtaccgc
      961 cgccgcgccc gttgggcttg gcctgccgcg taacgaagtg ttgtggatgc tgatgatggc
     1021 agttattggt tttggcgtga tggtgccagt cgccggatta ctggctgatg cctttggtcg
     1081 ccgtaaaagc atggtaatca tcaccacgct gatcatcctg ttcgcgctgt tcgcctttaa
     1141 cccgttgctc ggttctggca acccgattct ggtttttgcc ttcctgctgc tggggttaag
     1201 tctgatgggg ctgaccttcg gtccgatggg cgcgctgtta ccagagctgt tcccgacaga
     1261 agtgcgttac accggagcat cgttctctta caacgtagcg tcgattctcg gggcttccgt
     1321 tgcgccgtat atcgcagcct ggttgcagac taactacggg ttaggtgcgg tggggttata
     1381 tctggcggcg atggctggct tgacgttaat cgccctgctg ctgacccatg agacgcgaca
     1441 tcagtcgttg taataagagt gaaaagcctg atgcgctacg cttatcaggc cagaatttcc
     1501 atacaatata ttgaattagc aaggatttgt aagccggagg cgttcacgcc gcatccggca
     1561 tgaacaaagc acactttgcc aacaatctaa atctgtttaa ttgccggatg tcagacatcc
     1621 ggcaattatc atcacttctt catctgcgac aaaattgtcc tgcactgatt agcctcccct
     1681 tcggaaggtg agatcagcgc cagtaacgcc gccgccggtg ttaccagcgt tgccagtgcc
     1741 gccgcaacgg ctccgcgagc aatcaacggc ccggctttca ccccagcctg cggattttta
     1801 aacgtcccac gtacatacag cggcgaacgc aatgtgataa tccgaatccc tttactctcc
     1861 ggatcaatag tcaaatccag ctgttccgaa gcaaaacttg ccgtaccggt aacgttaatc
     1921 aacgcgttct cagtatcgaa agcaaaaatc tgcgggcgcg ccacgccgtt ggcaatatcc
     1981 agattcgccg ccgcacagtt cacccgcacc tcatcatcac caaatatcgc accgacaatg
     2041 tagttaccga cattcagccc gacaatctcc atcaggttgc ggctcaccag cccgtcattc
     2101 atcagcagtt tcaggttgcc gttactgttg cctaaaagcg ccgccaccga gttaccgcta
     2161 ccgcgtagtt ccgcgtcacc gttcatttcc cccagcgtct tctgcatcag ttctacatcg
     2221 ggcatcagtt ctttcagttt cagtcgacga gcctgaatat ctgcccgccc ttgcatcggt
     2281 tttttatcac cttccagatg aatattcgcc gcaatgctgc cgcctgccat gccaaatttc
     2341 agcggttgca ggcgcaggtc agcatttttg aggatgatat gagtagaaag atcgctaatc
     2401 ggcaggctac tgccatgctc aatgcgccgc cctttgaagc gaacatcggc atccatgacg
     2461 tcccatttat cggtttcgaa gcggtcataa ggcagcactt tgcccgcagg ctgaacgctt
     2521 ttttcaccct tcttctgttc agaccgtttc gacttttcag ctcctttccc ggaatcaacg
     2581 ccaattaacg gccctaaatc tgccagccgc aattgccgcg actcaacatc accttccagt
     2641 tttggtcgtg gcttgccggt ggtgtagacc agagaaccgt ggatatcgct atcgccaatt
     2701 cgcccattaa aaccgcgata atcaaagacc gacgattttt cagtgtcgat tttcgctacc
     2761 agccgaccat ccgtttcaaa cggcggggta tcgggcagca gaacgcccgt cagttcatag
     2821 agatcaccca gcgaatcgcc agaaaattta agccgtaaat cgacaccacc catcttcatt
     2881 gggtcattca caacgccatc aaaagcaaca cgggtgttac cagagcgaaa atcagcctgt
     2941 acaggaaatg gcgtcccttc gccacgcagc gccagcatac cgcctatttt tcccgtacca
     3001 gtgagcggct ctccgttata acgtccctgc gccttcaggc caaaaacgta atcgcccacc
     3061 ttttctttat ccgctttacc tttcgatcca gtaacttcgc tgaacggcag cggcttgcct
     3121 aacggatcga caaaaatttc tagatccgct ttgcttactt tgtcatcaat ggcgatccgc
     3181 ccttgatcga acagaatatt atccagccga aacgaccatg ccgacggctt tgcattcgtg
     3241 tctttgttgt catcgctggc gagattaaat gtccagttgt tgttcttttc tgagaggcga
     3301 atcaggcgtg cgtcgggctt ttcgagcttg atccacggca accagaccgt tttggtcagc
     3361 agcgccagcg gggccagcgt cgcttctact cgcggcaaat gcaccattgt gacttcggga
     3421 atatccggtg gattgccaag aatgatgtct tccgcgtgta catggggcca cggcacccag
     3481 ctgcgccagc cagtttcttg tttttgccgc tcccacacca cgcccagatc gccacggata
     3541 gcgaacggac gattcaactc cgcagagact ttctggttga tggtcggttt gagtcgattc
     3601 cagtcaaaag ttgcaatcaa aatgatcgcc acgacaatca acaacaagaa agcccctgaa
     3661 atcgcagcgg ttattttgcc tgccttgctc atcgttcgct tcctcatatt cttcctgtgc
     3721 cagtcctaaa gatagtccag ccaggcggaa aatgaggcag cttatagcgc cagaaccgcc
     3781 gtattcagcg tttctatcgg tgccgggcgt gaaagaaacc agccttgtgc ggcgaatgcg
     3841 ggcgagttct gaacatcacg ccactcttcc ggcgtttcta cgccctcgac aatcaccccg
     3901 cgacaatagc gattcatgag atgtaaaagc tgagaaaaga gtgtgcggcc ttccggcgac
     3961 tgacgcagca tcacgaacag ctctcgcgcg attttgatgt agtcataacg cacttcactt
     4021 agcgcagaga aatttgccat cccggtgcca aaatcatcca gccacagcgg gccaaattca
     4081 cacatcgagg caaaggttga atctttcggc agacggatat gctccaccag ttcgaaacgc
     4141 agccagggaa gacgctcaat ttggcgcagg atttttggtt gctgacgcag ggcgatgagc
     4201 gtagggccat caatattgac cgatgccagc aggtcgtgct ctataaagaa gtcggctttt
     4261 tgcgccagca aatcaatctg ctctttcaca acctccatcc gatggctaac ggtgatttca
     4321 gtaaaatagc gatccggcgg caggcgttgc gaagggttca agggatgcgt gaccaccgtt
     4381 aatagctcca cggccattaa ccgtccgcat gtttgataga tcggctgcca ggtgtaagca
     4441 cgctcacact gcaaccaaaa acgccgttcc tgcaagctct cgatgcttgc ttcagggttg
     4501 cttattcgct ggataacctg ccttatcatc aaagatgtcc tgtgtttaaa agtgatgctg
     4561 cccggactcg acaaagatta tcggcgcgca agggcagaac tttaccgcct ggtcggggta
     4621 aatttcccca ctccacaacg gaatctttca ggaatacgtt ctggctcaaa aaaataatgg
     4681 aacactgttt taatatggtt gaccagcaaa ccaccacagc gcaaactaac gctaattttt
     4741 tacagatcag gttcacgact atgtccaaaa agattgccgt gattggcgaa tgcatgattg
     4801 agctttccga gaaaggcgcg gacgttaagc gcggtttcgg cggcgatacc ctgaacactt
     4861 ccgtctatat cgcccgtcag gtcgatcctg cggcattaac cgttcattac gtaacggcgc
     4921 tgggaacgga cagttttagc cagcagatgc tggacgcctg gcacggcgag aacgttgata
     4981 cttccctgac ccaacggatg gaaaaccgtc tgccgggcct ttactacatt gaaaccgaca
     5041 gcaccggcga acgtacgttc tactactggc gtaacgaagc cgccgccaaa ttctggctgg
     5101 agagtgagca atccgcggcg atttgcgaag agctggcgaa tttcgattat ctctacctga
     5161 gcgggattag cctggcgatc ttaagccaga ccagccgcga aaagctgctt tccctgctgc
     5221 gcgaatgccg cgccaacggc ggaaaagtga ttttcgacaa taactatcgt ccgcgcctgt
     5281 gggccagcaa agaagagaca cagcaggtgt accaacaaat gctggaatgc acggatatcg
     5341 ccttcctgac gctggacgat gaagacgcgc tgtggggtca acagccggtg gaagacgtca
     5401 ttgcgcgcac ccataacgcg ggcgtgaaag aagtggtggt gaaacgcggg gcggattctt
     5461 gcctggtgtc cattgctggc gaagggttag tggatgttcc ggcggtaaaa ctgccgaaag
     5521 aaaaagtgat cgataccacc gcagctggcg actctttcag tgccggttat ctggcggtac
     5581 gtttgacggg cggcagtgcg gaagacgcag cgaaacgcgg gcacctgacc gcaagtaccg
     5641 ttattcagta tcgcggcgcg attatcccgc gtgaagcgat gccagcgtaa aagcagcata
     5701 tgcaaaaggc tggatagagt gttacccccc tatccagctc atccataaat acctgatggt
     5761 tgtgctaact catcaccatc aggcattact gtacaggtgg aatatccgtc acttcaggat
     5821 gtacgtcatc cgtggcgaca gatgtggcgg cagccgcagt cgatggggcc atgatttgat
     5881 cccaggccgc ctgcaacgct ttcatgttaa attccggctc gcctttcggc tgcagcagta
     5941 ttaacgccat atcattcgac aactgctgac gcagatcctg atttaacatc tccaccgtca
     6001 ggctgttaag gaaatcctga cgcagtttct gatattgctc cggcgcgata tcgacgacct
     6061 gattttgcaa cgaacgcatc cgctgcccca tcagaatatc ggtatcagct cgcgcatagg
     6121 cggcaaacag tttctgcagc tccagtttct tttgcgccac taacgcattg aactcttctt
     6181 ccggcagacc tttatcgcga accttcgcca gttcacgcgc caccagatta aggttgctgt
     6241 tcagcttgtc gtttggtgat tcgatgttga tggcacactg cgcacgcaga tacagcacac
     6301 gacagtcaaa tccaagaccg atgtctttac tgttactggc gcttaacgct tgctgaacat
     6361 gccagaacag cgcctcacgg gccaggtccg cacgccagta gcgcagcagt gcggctgatt
     6421 cacgaatcgg ctgccacggc gtatcccaca tgattgataa ccggtcctga cgcaccgcgt
     6481 cagtcataat actcaccgct tccgcacgca gcggagaaag cgtcggcacc ggagccggtg
     6541 tttcacgttt gcctttcagt tcgccaaacg ttttattgat ttggtcgacg accgagcgcg
     6601 catccacgtt tcccaccacc agcagcgtca ttgcatccgg ggtataccat ttctgataga
     6661 aatctttaat cttttccgct tcaacgggtt gtttcagcgg atcggcagga tcgtgaccta
     6721 acaaggttga tcctttcaga cgatagcgcc accagccctc tttagtatcg gcaggccagg
     6781 ttgccaccat gtcctgactt tgcagcgcgt ggttgatggt ttctggtgtg atggtcagtt
     6841 tgccagtggc atttgccaga taagagagcg cttctttcag caagtcgtta cggttattgg
     6901 gcaaactcag attaaacagc gtggtgtcat aagagacaat tactggcggc atcgggcgtt
     6961 tagggtcgat cccctgctgc cacaatgaac gcgcctgtgc tgcttcaagg ccaccgcttt
     7021 gcgttaacgc aatacgaggg atggcgtggc tgtaaccgct ctgttgtgta ctttcggcga
     7081 gcgaaccggt attaaccagc aggcgaattt caacacgatc gctaggacgc tggggcgtgg
     7141 tcagcacttg ccactgtaaa ccgttggaaa gcgtcccctg ttgccatgct ggatcaggct
     7201 ggagcgcatc tgcctgcaca tagccagcag tggccatcat cagcaaaccg cccgctaaaa
     7261 gtcgaatttt tgtgccctgc atgtgaaccc ctgatcaact atcctggtaa taaaaaaact
     7321 gcccgttgtc ggtcagtcta aatgacgtta aaaacacttc gtgttagacc gcaagaacat
     7381 gaaaatgtca cggaagaagt gaaataaacc tgaactcacc caggtggtaa gttcaggcac
     7441 aggggtcaat tatgcgcaaa cacccgcact cggggaaggg agtgcgggca taagtgatga
     7501 gattaagagg ataattcgtg cgttttgcca tccggcgcac gattattcag cacatcgtcc
     7561 agttttttgt ggtccagttc tttcacccac ttagcaacga caatggtcgc tacgccgtta
     7621 ccgaccaggt tagtcagcgc acgagcttct gacataaagc ggtcgatacc aaggatcagc
     7681 gccagacctg ctaccggcaa atggcccacc gcagagagcg tcgccgccag cacgataaag
     7741 ccactgcccg ttaccccggc agcccctttg gaagagagca acaacacaat taatagcgta
     7801 atttggtgga cgatatccat ctgactgtta gtcgcctggg cgataaacac cgccgccatt
     7861 gtcaggtata tcgatgtgcc atcaaggtta aacgagtagc ctgtcgggat gaccagcccc
     7921 accaccgatt tccggcagcc gagtttctcc atcttgtcga gcatacgcgg cagcgccgac
     7981 tcggaagaag aagtgcccag tacaatcagc agctcttcac ggatgtagcg gataaatttg
     8041 aagatactga aaccagtcgc tttagcgatt gaacccaata ccagcaccac aaacaggata
     8101 caggtgatgt agaaacagat aatcagctgc cccagttgca ccagtgtgcc gacgccgtat
     8161 ttaccgatgg taaacgccat tgccccgaac gcaccaatag gtgccagacg catgatcata
     8221 ttgatgatgc cgaagatgac ctgcgagaaa ctttcgatga cgttaaaaat cagttggcct
     8281 ttgctgccca gacggtggag cgcaaaacca aacagtacgg caaacagcag cacctgcaga
     8341 atgttaccgc tggcaaatgc gccaatgacg ctcgccggga tgacatccat aatgaaggcg
     8401 acaatgccct ggtctttcgc ctgatcggcg taaaccgcta ccgctttcgc atcaagcgtt
     8461 gccggatcga cgttcattcc ggcaccaggc tgcacgacgt taacgatgat aagaccaata
     8521 atcagcgcga tggtactgac aatttcaaag taaagcagtg cgactgcgcc ggtacgacca
     8581 accgccttca tgctttccat gcccgcaatg cccgttacga cggtacaaaa gatgacagga
     8641 gcgatgatca tcttaatgag cttaacgaac ccgtcgccaa gcggtttcat ttgctcgcct
     8701 atttcagggt agaaatggcc aaggagaata ccaatggcta tcgctgtcag gacctgaaag
     8761 taaaggcttt taaacagaga ggttttcata gggtgtcctt tagtaaaacc acaggtcttg
     8821 taaggttatg gggtacctgc ggccctaaaa taacacccag acaacatcac agaaatgtac
     8881 ctggatcata attgaaacaa aaaggttaaa aagtttgagc tggctcgcac aaaccagcac
     8941 tttttaaagt tttgtaatca gtttggggta gctacttttc ttccaggtaa ctctcttcga
     9001 agatttcaat agggagtggg cgagcaaaaa ggaagccctg ggcaatacca acgcccgctt
     9061 ttgccagcca gtcgcgttgt gcttcagtct ccacgccttc ggcaatcatt tgtaagttca
     9121 ggctttgcgc caacatgata attgcagcaa tcatgctgct atcttccggc aagccttcaa
     9181 caaacatttt gtcgattttc agtacgtcga ttggcaacga tttcatatgc tgcagctgac
     9241 gcagccctgc gtagcccatg ccgaaatcat ccagcgccac ccgaactccg gcattgcgca
     9301 gcggacggag gattgccacc gcagcatgag ggtcgtcaat acgtcggctt tctgtcactt
     9361 ccagaatcag tgttcccggc tgaatgcgat agcgggttaa cagttccagc atatccgcta
     9421 ccatattcgg gtgcatcagt tgtagcgctg agaggtttac cgacaagggc agcataatgc
     9481 cgcgctcttg ccaggcagca agcagtcgac aagactcttc cagcacccag tgaccaacgg
     9541 taaccatcag cccacagcac tcaatgcgat cgattaagcc atccggcaga tcccaactgc
     9601 catccggttg ctggatacgc agtaacactt ccgcactgac cagtttaccg ctggtcatct
     9661 cgacctgtgg ctgtaaccaa atcgcaaatt gatgattttc cagtgcatta aggatatcgc
     9721 tctcttccgt caaccgctgc tgggcggctt ccatctgctg cggatcaaag aactgaatct
     9781 gatttttgcc tttatggcga gcggtaaatg ccgcagaaat agcgcgactg taaagctgtt
     9841 cggcggtgag atcgccgtag aacatcgcca cgccaatgct acagtgcgga cggagttgaa
     9901 tacgttcaat cggcaggcgc tcgctcatga tagtgagcac ttgctgacct aatgtgatag
     9961 cgtgccacgg ttcctgtaca ccgttggcaa tgacagcaaa gtcataaccg ctaatctgcg
    10021 cgaggatcat gcgtggcgac agtaccgatt tgagtttttc caccagcgtc agcagcagaa
    10081 tttctcgttg cgcctctttc agcacgcccg cagtatcacg cagggtttca caggtgatga
    10141 tcatcagcgc ggtggtttgt ttacgcgcga caacctgctc cagcatctcc atcagcaagg
    10201 ctttgttcgg caaatccgac accgggaagc gcatcgcgtt ttcgttctgt tcttcataat
    10261 ggcgctgcag caattgctgg ttgaggttgt aactgcgcac cagcataccg atttcatcgt
    10321 cctgatgcag acgtggtaat gccagttggt gaccaacaag ctcctgggct gggatggcgt
    10381 taagttcgcg agcaatattg cgtaacggat gtaaaatcag tcgattaata caccagctga
    10441 tggcgacggt caggataagc gacaaaagta agtaaatggt cactaacgtt gagagggtgc
    10501 tcatcacgaa cttatacata cggaaggaat ccgcctgtag caccagatag gcaattggct
    10561 gcggatttgc cggacgttcg agcgagtaga cgcccagcga gatttgaacc ggtagctcaa
    10621 acaggcgagt aaccattacc ggcaccggac gctctggaat aaaactttta cgcagcgcct
    10681 ggaactggtt gggcagcacc acatcggcac ggctgaccac gcctgccggt ttaatgctgg
    10741 caagaatagc ttccgcttcg ggaatatcgc ctttcaaaat agctgaagat aagggttcgc
    10801 ggacagagcg agcaatgctt tccagttgcg tagccgtgtt atagcgattc tgctggacca
    10861 gatggaacag caaaacggtg caaaaaataa aaacgaacac caggacaacg gctgccacca
    10921 ttgccatctg cttgattgtt aacgagcgac ttacgcgcaa attgtctccc cccggaaatc
    10981 ccagattgcc gcccgagtat acccgatcgc agcggcatta agagaggcgc tatctgaaaa
    11041 cttaccagtc ggcgtaaggt atcagcggct gcggcggtaa atccatgtca ccctgccatc
    11101 cggcggcgga ataacgtacg tagagcagga aatggctggg tgcgtaatct ttcgcctgct
    11161 ggatatcaat tgccgtgccg acaaaccagt tggaagtaac acgtcgttca agtaatgccc
    11221 gcgccgtata gccgaagccc tgactgctgc cgccatcgtt ggattgccgc gcagcttctt
    11281 cctgccagtc ggtcgggatc agattcatca gcggataacg cggcatggtt ttggtgcgtg
    11341 aatgcgacca cgagccagac gcccccagct cccacgacca gttttccgtg cgctcccgcc
    11401 acatcaccgg tatggcaaac gacaggtatt cctgcgggct gtagtaaccg ccctgaccga
    11461 gtgagtagcc actcaggtct ttgtcgtaat gccagatcat gttgttcagg ccgattgtga
    11521 cgcggcgatt gttctggtta atgaccttat agtaatagcc cgtcatccag cgcacacgcc
    11581 agttatcttc gacattttta ccggttaact ggtcgccact aagcgatgcc cagacgccgt
    11641 ttgcttcacc tttatcgtag ctcaggctta gccccacgcc gtcggcacgc acgccgcccc
    11701 attttttccc ggtattgctc ggggagtctt tttgcccacc aaaggccagc aaagaactgg
    11761 agatgggccg acggtgggcg ttaacggtgt aacccagcgg cccgatatca tcgctgtaac
    11821 tgatgccgcc gaccacatcc accacgttga agcccatcgg cgtggtgccg atatcccagc
    11881 tccaggtgtc gttgcgccaa ccgaccgcca cgctggcacc ggaatccgac tggctgcggt
    11941 tgccgctaca gtcctgtaat gtacaggtgc cccagttgtc atcccattta ccatcggcat
    12001 tagtggagaa actgccgacg ttcatattga cgaaatcact gcgaaagaac atccgcccgt
    12061 cagaatacgg cgcatccacc tgcaacatgg tagtgtgcgc tttcagatcg gagtaaccac
    12121 cggtgccgct cgaaccccag taatcatgct caagggtgac gttaagatcc tgctggcgat
    12181 agaggtccgc cgcatcgctg cgcacgccgc gtttcagcca gtcatctttc tcgtcattgc
    12241 gggtcagtcg ggtaaaggtg tcgttatcca gcggacgcgt cgtggtcaca ccggatgcaa
    12301 ccatcgcgtc tttgtaggtt tccagcgcct gcgttggatc gcccgcctgc gcttcaaact
    12361 tcgcaccatc acgcagtacc atcgcacttt ccatcgacgg cggctgagat tttgccagcg
    12421 ggatcaactt attaaacgtc tgctgcgccg ctgcggtatc gccaagctgc gcctgtgcca
    12481 gcgccacgcg ccgctgagtg ttcagcgagg cgttatcggt ggcaggcagc ttcgccagct
    12541 ggctacgtgc cgccgctttg tcaccggcag caatatccac ttccgtcaga ccaagaatgg
    12601 catcggcgtt agttggctcc cgcgtcagga cattctgata tgcagcgcga gcggcggtgt
    12661 aatcacgtcg ttgttgcgcc cagtcagcca gcgtgaggtc tatgcgcgtg gaaggtggtt
    12721 gctggcgcag catcgcttcc gcttcagctt ctttgccgct ttctcgcagg cgattagcgg
    12781 tttccaacac ctgatcgctt tgcagtcgat taaccagctc ctgaatattg ctgctccact
    12841 gcgcgcgcgg caggctgttg atatgcgcca gcgccgctct gtcctggtca tgaccagaga
    12901 gatacagccc gtaagcgtaa acctgctccg ggtcgttcgg cttctgctgc gccagattac
    12961 gcattaacgt atcggcctgg ctgcgttgtc cggcctggct gcgttgtccg gcctgccaga
    13021 gatcctgtga aagtcggtaa gtaatccata cactgccggg gtccagcgcc agtcgttgcc
    13081 gctgaagtgc tgccgcctgc gcccatttcc cctggttttc cagtgcctct gcctgctgtg
    13141 ccagacggtc gttttgcagg ctgcgttcga tatcatcaat gctgcgccgc tggctggcag
    13201 agagcgaggc gatatacgct tcggcttttt ctggcgactg ctggcggtaa atattcgcca
    13261 gcccgcgcac ggcgttagtg ttgccgctgt ccatacgcaa ggtctgctgg taatagcgtt
    13321 cggcggcggg ataatctttg cgcgccatcg ccacatcgcc cagcccaagc actgcatagc
    13381 tgtcggtgtt atcgacatta cgtgcctgct ggaacagccg ttctgcccgg tcaggattat
    13441 tggctttcag cgcggcatcg ccctgctgga tcgccagcca gtagcggttt actttcagca
    13501 gactgttcca tttgtcgtta ttgctgttgt gcggatccag cgcgagggct ttttccagat
    13561 tcgccaccgc attagcgcga tcgcctttct gagaatacgc ctggcccagc gcccccagag
    13621 cctcactgtc tttcgggttc gcccgcaccg cctgttgtag ttcgggaatg gctttacccg
    13681 ccatgccaga gtccaccgcc gctaaacctt gcgcacgagc gcggaaagca gggtcggcca
    13741 gctgtttttg ctgttctgcc agttgcgatt gtgcagcagc tacgctatcg ccatcgctaa
    13801 agatcgagag atattttttc agtgcctgaa cgctggcatc gctgacaggc atgtctttaa
    13861 tctgcccgta ccagatttta gaggcccctt cgcgcccggc gttcgatttc gccatctgtt
    13921 ccaggacggc aaaaccttca tcacggcgat cgctactaaa cagtaatagc gccagattgt
    13981 tttgcaggcc cgtgttgccc ggcgtatccg cattaatgcg ttttagctga ttaatcgctt
    14041 cgccacgtcg ggccggaatt ttcgccaccg tactccagta ctcgacagca atgtcacctt
    14101 ccggcggcgc accgttgaac agtttgttgt aactcgccac agcttcttcc gcatgaccag
    14161 tcgtcgcctg caatcttgcc tgttgcagtg cctgacgacc atccgacgtg gaaagcagca
    14221 tcgtagtccg tgacgattta tacgcatttg aactcggcgc taactgcgac agccgatcga
    14281 gctgtttttg tgcgccatca atatcgccct gacgtaacag agaacgaaaa cgggcagcaa
    14341 cgacgtccgg gttattcgga tcaataagtt ccagacgata caacgactgt tgcaccaggt
    14401 cttcacgatg ggtcgcttcg cctaaccgaa cttgctccag caactgttgc tgagcggttg
    14461 gtgctgcctc gaccatcggc atgacggcca gaccgaggga aagcgtgaat atgtttagtg
    14521 tgaatttgcg cattcctggc cccagtcagg taataactca ccttttgtcg agaagcggaa
    14581 acggtgttga tcccagcctt gtccaaacag ggtcagcaca tagttgtaat aggcatcgct
    14641 gccaggaaag ttatcggcca cgcgctggcg ctgaacggcc tgcgcatcgc ggttttgtaa
    14701 aaagggcagc atggcggcag aaaaaccgac cggtccttta ccctgcgctt tccccgtagc
    14761 cacatccact ttttccggcg gataaccgtt tttctcagtg aatgtcgcca tcggtttaaa
    14821 ccggttgagc atccgcgctt tttgcggatc gctgtcaggc atcatgccta cccacatgta
    14881 aacgcggata gcgtcgtagc tgctgatcaa tgttttttcg gcttttagct gccagccttt
    14941 gtctttctca tagcgcaccc agtctggcga aaagcctttc ggggcggttt ccagcaataa
    15001 acgttgattg gtttcgcgca gcgtggtcca cggcgcgcca aagcgggtga aatactgcgc
    15061 cagcgtcggc ggcaggtagc tggggttaaa acgccagctg ttatcctctg caaatcccac
    15121 tttgcccggt aacaacatgg agcccagccc aggcaccgtc accacttcct cccgcgcgat
    15181 acgttttagc aatgcgctgc cgatgtcggt ataacgctgc tctttccaca aacgccccgc
    15241 ctccagcaac gcccaggcca tccagacatc accatcggag gccgaattgc tgtccagcac
    15301 ttcccactta ctgttctctt tcttgcccca cagccaggcg ggcaaatgtt cttttaaaga
    15361 accctgtgcg agattgttct gcgtccagtc gagaagatta tcgaaagctg cacggtcgtt
    15421 agccgccagg gcaaagaaca tgccgtaact ttgcccttcg gaggtggtga ttttgcgcgc
    15481 gtcgctgggg tcgatgacgc gcccttcctg actgatgtaa tcctttttaa actgctccca
    15541 ggcaggccag gtacaggctg cctgaacact aaaggcagcc agcagcagca tcgtcacgag
    15601 tccactacgc aacacattca tcttcaatta ctcgttatcc gggttaagac gacgacggct
    15661 gataatacgc agcagacgcc acagtaccca tgccagcagc acgacgctga ttgccgccag
    15721 caccgccagc agaatcggat ggtttgccag cgcataccat aagcgttcga accacggcag
    15781 atggccaacg taatagacgt cgccaacacg caggctattg ataccggact cgcggatcac
    15841 cgcgaccgaa ccgaacatgg tggcgcgttt gccgctatcg ttcaccgcat cgttaagcat
    15901 ttcataaccg cgtgggctat ctgccagcag cgcaatcacg ctacgctggt cgttatacgg
    15961 cgactggaag ccaatcaccg ctgccatcgc acctgaagag gtcagcgttg actgagtttc
    16021 tgccgcgcga tcgctctcgt ccggtacaat gccggggaac ggggtctggc gcatcggcgt
    16081 tttcacccag ctttcggtcg cctgcaccaa caggtcgatc tgcttgtcgt ctttcagttt
    16141 gtccgggata ccaccgacga tcatgatgtc ggcatctttc ccctgaatag tgctgccatc
    16201 atcggtcacc gtcaggttaa tcgccgggaa gcccgtctgc gcaccgataa agccaacggt
    16261 attcagcaag gtttccatct gtgcttcgtt aggcgctttc ggcatcacgg tgatggtttg
    16321 tgacagatcc gccatccggc tgaacgggaa gcccgcgtta gcaaaggcgc gtagatccgg
    16381 catcgggatg aagtggtaat acttcgagaa gtcgatggtg gaatcatcac caatcaccac
    16441 atgattctgc accggctgga aggtaatgca gttatccacc gaaccgcccg gcatcgggtt
    16501 catatactca aagtcaaagc gcagctggtt ggtcgcgccc agtttcagcg ccggaataga
    16561 gacatccgtt ttgccatcca gcaaaccttg taataccgga atacgcagca gcaggcggtt
    16621 cgcctcctgt ttactgctca ggttgaagga ttgcaggaac tggttattca ggctgatatc
    16681 catccgtgag ctgtctttca ctggcggcat ggtgtagcga taattaatat ccatatcaat
    16741 gccggtactg cgcatcaggt agagatccgg tggcaggttt agcgaaacgt taatcgctgc
    16801 tggctcaaga ccgctggatt gtaactgttc ttcataggtt ttcagttcac caaaggtaac
    16861 cggacgatcg gtacgcaccc agttcggcgc atcgtacggc ttacgcggta gcagcggttt
    16921 cacttcattc actaccacgc tttcaccacg gaacagaatg ttaccctgag cgataccttt
    16981 cgctgcctgc aacaggtctt tgtcgtcacg accaaacacc accagcagtt tgacataagg
    17041 attctgcgga tggttgatca tttcaatcac cggggctttt accgccggat gatcgcgcag
    17101 gaagtccggg cgtttgtcat tggtggcaaa aacgatggca ttgcgatccg gcaattggtt
    17161 atagagcacc gggaagttct gcccacgcca gccagaacgc gaaccaaacc acgaggcgac
    17221 aatggcagat gcttgttgca gcccgacatc cggcgcaccc gcaaagacca tcggcaaggt
    17281 gttagtacgg ttatcacgcg ggtcaaagaa tggcaccggg aagtgtgaca ggtcattctt
    17341 cacattcagg gtctgatagg tcagatccag tccactgctc cgcccaacat ccagccaaag
    17401 cgtggtgctg gccgggtttt cgcacacgtc ctgataatga ccgacaaact ccagccgcac
    17461 acggttgaag tcggtaataa acagtgggtt aattggcatt tgtgccagcg tttttttacc
    17521 caactgttct ttggtcactg gcagcacgcc catcagttca tcattgagat aaacctttaa
    17581 ctgcgactgg acaggcagta atgatggcga tggcgtgtat tcgaggttga gcatcgcctt
    17641 cgtcaccact tcatcgctgc gcataccaaa ctcaatgctg ccgttcgggt taatgccacg
    17701 tagcaccatg ctgcccggcg gcggcgcaat ttgcgcaaag gtcagcttca catcacggga
    17761 aggaccgttc tgcgccacga ctggcgcatc tgcgccctgc acgcccggca tcacttgccc
    17821 cacctgcgga ttttgctccg cctgggcggc tacagctggc tcagcattga tcagtggttg
    17881 cgttgctggc gtcgcctgcg tcatgaaaga ggggaacgca ctcatcccca gagccactgc
    17941 acaaatccag aatatttttc ttttcatcgc gttatcatca ttgttgagcc aaagcctgat
    18001 ccgatggttg tgccgtttcg ctccgctccg ggcggcgcgg aataaacgat acaacccagg
    18061 aaaccagaga agtcagcaca cggaatatgc ccttcaccga agaaggcgca aattccgcca
    18121 gatggcggta gccacggaag ccgagcttca gaatatccag cagactttcc agcggcttat
    18181 cttccgggta gctgtcctgc cagagcgccc atgtatccgc acgggcaaac gtacactgca
    18241 caaaatcgat atgttgctgg gtagtgagcg gcattaattt cagcccaact tcattaccca
    18301 tcacacgcgc cacctgagcc gggaagacgt attcctgctg accgcgttta agcaacagat
    18361 tcactttctg cccttccaga atctgcgcct gaccgttgat cttgatcccc aaaccaccgt
    18421 cggagaaatc ctgaacggta cacgagaaga ggtgaccatc ttcgcgggca attgctgcgg
    18481 gcatcgtcat ctccacgcgg tgcgatcggc gtacctgttt gctttctacc gatactgcga
    18541 ctgcgccgcc gagaataatc aggttgtaga atacccacac catactgacg accacggtga
    18601 gcatctcggt tggcggacca tagaagtagc gccagatccc aaccgctacg cccaccaggt
    18661 tgagcagaac aaggaagatg tagggccgcg agatcaccca gtcgacgtac tcttcttcca
    18721 ccagtccacc tttggcggtg acgttaaatt tgcctttgtg cgggttaatc agcgccacca
    18781 gcgtcggtgg tgcgatatac cacgccagca ctgtttcgta gatttcactc cagaaagagt
    18841 ggcgatattt gccctggatc ttcgagttag tcaggctggc atggatcata tgcggtagca
    18901 cgaacagggc gatcatcaac gctggcgcgt agatgatgta ggcatgaaac agcaggaacg
    18961 ccagcggcgc agtcaggaag atcagccgtg gaatgcccga caagaaatgg aacatggcgt
    19021 tgacatagca cagccgctga gcaaacttca gccctttacc ggtgagtggg ttatcgagac
    19081 ggaagatttg caccatcccg cgcgcccagc gaatacgctg accgatatgc gccgagagac
    19141 tttcggtcgc cagccccgct gcctgcggaa tacgcatata cgctgaggtg tagccacgac
    19201 ggtgcagacg cagagaagtg tgcgcatctt cggttaccgt ttcgacagca ataccgccaa
    19261 tctcatccag cggcttacgg cgaatcaccg cacatgaacc gcagaagaaa gtggcatccc
    19321 acatatcatt gccgtcctgc accagaccat agaacagcgt gccttcgttc ggcgttttac
    19381 gaaaacgccc caggttgcgt tcaaacggat ccggcgagaa gaagtggtgc ggcgtctgca
    19441 tcatcgccag ctgtttctct ttcaggaacc agcccatggt catttgcagg aacgatcgcg
    19501 ttggtacgtg gtcgcagtcg aatatcgaca cgaactcgcc tttggcatat ttcagcgcat
    19561 tgttgatgtt gcccgctttc gcatgttcat gagtggtacg ggcgatatat ttcaccccca
    19621 cgttttgcgc aaactggcga aactcttccc tgccgccgtc atcgaggatc cagatgttca
    19681 gcttgtcttt cggccagtcg atacccagcg aagcgtaaat ggtatttttc accacgttga
    19741 gatcttcgtt gtaagtcggg acaaagatat ccaccgacgg ccacagcgac atatctttcg
    19801 gcaatggcac cggctgacga ttcagcggcc atacgacctg gaagtagccg agcaccagca
    19861 ctatccacgc gtacgtttcg gcgaacagca gaataagccc gcacaccagg ctgaccggat
    19921 cgtcccagtt taacgtcgag gtgtaacgcc accagatata acggcaagaa acggtcagcg
    19981 acagcacaat cagcatcagc gccgagaagc gccccggcat ccgccgtacg atcagcgcta
    20041 ccccccacag cagcatcagg aagataaact gcgctagcgg gttaaacggc tgagtaacgc
    20101 agattaacgc cagaatcagc gagaaggtga cgatgatacc aaggatcaac cgccgcgccc
    20161 cggcactcaa atgaccgagc tcttttttct catcaagatg ctgtgtttta tggctaacgc
    20221 gctcaggcag ctcgttcatc cattgatggt aacgtccacg aatattttgt agacctgaaa
    20281 atgcccgcct gcgcggtttc ggcgtttctt tgcgcgatgc accgatcagt aaccagcatg
    20341 tttgaataag ataacggacc gggtccagcg gacgcggacg cgaggcgttg atatgcggat
    20401 acaggttttt atgttctgcg cgaatacgct gccagcgcgg gtgctccagc ggaataaaaa
    20461 tccaggccag gatcatccag aaacagccga gcgtcgcgct gaaagccgac gcaccgtgac
    20521 gacgataatc gcgataacgc ccaataagcc gtgcgttgac cggcgggata agcaaccacc
    20581 gggtcaggat actcatgatg cactcccgac tggcgttttc agcccggaat aattcaacag
    20641 gcaccagttc gccagcgtca gtatctcttc agccgccagc gcatcactgc gatattcacc
    20701 gactggttgc ttagctgcca gacattcagc catcgcttca tcacgatgaa tgagcatcgg
    20761 cagcaatcgg cgctggcttt gcaaccaaag ctggtaaata tcgtcctgaa cctggctgcc
    20821 aatacggaag tcattaatca aaatatgtgc accatccggc agcgcttgct gatgcagtcg
    20881 gatatggcag ttggcatcca cattgacgat tgccagcgag tgatcgcaca aactcagcag
    20941 ctggtgggtt atctgcgagg catcacgcgg taagtcaatt aaaatccact ggtaacgccc
    21001 gctggctttt agttgctgta agccggtgca aatatcgctc agccgggttt gccagtgctg
    21061 tggattttct tgttcttcaa tcgataactg accaaaaggc agcaaatcga gctgcgaggt
    21121 atagcgcaac ccggcgtcac gccagtcctg gccatccagc atagctctgg cccaaccctg
    21181 acggtgggta aaatcaacgt taaatgacag gcgcaacaag ttgtccgggc aggcatcgac
    21241 caccaggaca ttttctccca acatttgtaa tgaccaggct aatgcggcgg tg
//
LOCUS       DAZGHF010000080        21180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGHF010000080 DAZGHF010000000
VERSION     DAZGHF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.080"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(375..1571)
                     /gene="hemY"
                     /locus_tag="ACHK3A_003109"
     CDS             complement(375..1571)
                     /gene="hemY"
                     /locus_tag="ACHK3A_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO8741160.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1574..2779)
                     /gene="hemX"
                     /locus_tag="ACHK3A_003110"
     CDS             complement(1574..2779)
                     /gene="hemX"
                     /locus_tag="ACHK3A_003110"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO8741161.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQAD
                     APAAAPQGE"
     gene            complement(2801..3541)
                     /gene="hemD"
                     /locus_tag="ACHK3A_003111"
     CDS             complement(2801..3541)
                     /gene="hemD"
                     /locus_tag="ACHK3A_003111"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO8741162.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CVIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3538..4479)
                     /gene="hemC"
                     /locus_tag="ACHK3A_003112"
     CDS             complement(3538..4479)
                     /gene="hemC"
                     /locus_tag="ACHK3A_003112"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO8741163.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYESLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4866..7412
                     /gene="cyaA"
                     /locus_tag="ACHK3A_003113"
     CDS             4866..7412
                     /gene="cyaA"
                     /locus_tag="ACHK3A_003113"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO8741164.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     CNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7452..7772)
                     /gene="cyaY"
                     /locus_tag="ACHK3A_003114"
     CDS             complement(7452..7772)
                     /gene="cyaY"
                     /locus_tag="ACHK3A_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO8741165.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7820..9877)
                     /locus_tag="ACHK3A_003115"
     CDS             complement(7820..9877)
                     /locus_tag="ACHK3A_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741166.1"
                     /translation="MINTSRQDGFMHFRNVVCLLSCSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGTR
                     TDDNGGENSLDIKTLHQVKFRSPWKPDFLGLILRDANFLYVDGHRLADPESFRVLAQK
                     SWDQRGKFSTTFNPCVAVPFGPWDTLARTRTKIIINGEQLKADPETFTVVRWMPGSLL
                     TWRDKSGLHRKVLNQGNLDKDRANSCAAFTLQEHSVSWRKGPECQQEVLPGLDPEQFH
                     PLSNTVAQYQDKLYTIKKTGFGENRLNIVTLDNPGLVIDKRFNAGRTHGYLLTKLPAD
                     GEEEDGLQVFDSSGPLILMDYRVPDEHEAHLSDSPHYKKWYAHDDRYVYAFDGSQLWR
                     YPTPNTKAVRVKWKTKDSGYGYWANYRSGELDGRLLEDGAFIPTGIPYNSSVNIAQYS
                     DGEGSFLVGETDVSWRKLRSTDQQWSQWEKLPDIDPKAFHPITDRVAQYKNHLYIAKL
                     SPFGEDTLEIITLDSSAPFLKQRINAGKGHGYFMRSSRLRDDIQIFAIDGPLKTTERF
                     AYDNRYVYTWIGSQLYRTASPCPARTRNLDQNMFSSNDDIIILTTEEECHKTPDIEQT
                     LKL"
     gene            complement(10219..10773)
                     /locus_tag="ACHK3A_003116"
     CDS             complement(10219..10773)
                     /locus_tag="ACHK3A_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO8741167.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11115..11318
                     /gene="yifL"
                     /locus_tag="ACHK3A_003117"
     CDS             11115..11318
                     /gene="yifL"
                     /locus_tag="ACHK3A_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8741168.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVEM
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11355..12179
                     /gene="dapF"
                     /locus_tag="ACHK3A_003118"
     CDS             11355..12179
                     /gene="dapF"
                     /locus_tag="ACHK3A_003118"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO8741169.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12176..12883
                     /gene="yigA"
                     /locus_tag="ACHK3A_003119"
     CDS             12176..12883
                     /gene="yigA"
                     /locus_tag="ACHK3A_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO8741170.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDCWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12880..13776
                     /gene="xerC"
                     /locus_tag="ACHK3A_003120"
     CDS             12880..13776
                     /gene="xerC"
                     /locus_tag="ACHK3A_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO8741171.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13776..14492
                     /gene="yigB"
                     /locus_tag="ACHK3A_003121"
     CDS             13776..14492
                     /gene="yigB"
                     /locus_tag="ACHK3A_003121"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO8741172.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14576..16738
                     /gene="uvrD"
                     /locus_tag="ACHK3A_003122"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14576..16738
                     /gene="uvrD"
                     /locus_tag="ACHK3A_003122"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO8741173.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16783..17685)
                     /locus_tag="ACHK3A_003123"
     CDS             complement(16783..17685)
                     /locus_tag="ACHK3A_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO8741174.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LKQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17768..18532)
                     /gene="yigE"
                     /locus_tag="ACHK3A_003124"
     CDS             complement(17768..18532)
                     /gene="yigE"
                     /locus_tag="ACHK3A_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO8741175.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVG
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            18694..18750
                     /gene="ysgD"
                     /locus_tag="ACHK3A_003125"
     CDS             18694..18750
                     /gene="ysgD"
                     /locus_tag="ACHK3A_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO8741176.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18902..19852
                     /gene="corA"
                     /locus_tag="ACHK3A_003126"
     CDS             18902..19852
                     /gene="corA"
                     /locus_tag="ACHK3A_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO8741177.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19888..20385)
                     /locus_tag="ACHK3A_003127"
     CDS             complement(19888..20385)
                     /locus_tag="ACHK3A_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO8741178.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHFFIADNNEYQLTQENKIERVFNPHDFEK
                     DNFQQ"
     gene            complement(20382..>21180)
                     /locus_tag="ACHK3A_003128"
     CDS             complement(20382..>21180)
                     /locus_tag="ACHK3A_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO8741179.1"
                     /translation="TITLFDDFSADDMQYGDMEEQDFLSLGLSDISAKVDPYRLIKYH
                     FPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLAKMFSFFGQYKTLIQDLIDHFR
                     YGNGNSFHSQELNLSFHERINKYDYNSPIRVIKECIENDISSTPTIGYRPLLLQKIKT
                     ELLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGL
                     DVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
BASE COUNT         5037 a         5393 c         5429 g         5321 t
ORIGIN      
        1 tgcgcaggag aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa
       61 ttatctcata acaagtgtta agggatgtta tttcccggtt ctctgtggca taataaacga
      121 gtagatgctc attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc
      181 agagccgttt acggtgctta tcgtccactg acagatgtcg cttctgcctc atcagacacc
      241 atggacacaa cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg
      301 ctccgtaata agagcaggcg tttttttatg tatcagaaag gtcccggagg agcttgcctc
      361 cgggtgagaa agagctactg tggcgggttg ttctgcaacg ttaacatcaa accgtcgcga
      421 cgcatcgctg cggcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg
      481 taatcgtagg cgtccggacg ttgtttcagc gctgcgcgga aggcgagcga tgcttcctgc
      541 cattcgccgt gcttcatcag cgactgaccc agtgtgctcc acaacagcgg gcgatcgccc
      601 acgtttttga tctgttggcg cagcactttt tcaagctgtt ccgggttatt ggttttcaga
      661 cgcgggatcg gcagcagcag gcgatcgtca tactggcgtt tcaggccgtc gatgataatt
      721 tgctgggcag tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgtaac
      781 gccacctgat gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcacta
      841 ccgttatcgg cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca
      901 cgatgttctt catcaccaac atgggctttc gccattgatg ggatgatatc cagcagcgaa
      961 ctccatgcac cagtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt
     1021 ggcgtaactt ccagcagctt atcaacgccg tgacgtgctg catggttttc attacgggcc
     1081 agttgcagac gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctccgct
     1141 gcgcgttcca gatgttggtt ggcgcgtgct tcatcaccac gttgttgcgc ggcttcggct
     1201 gccagtagat agttcaccac cggttgttca gcgtgatcgg catttttcgc catcagcttt
     1261 tcaacttgct gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgt
     1321 gcacggcgac gcttacgtcc gacaaaccac ccacgggtgt gcgcgcctgt gcggaagatt
     1381 cgccgcagta gccactcaat ggcaaacagc actaccatcg ccagaatcaa aataatcgcg
     1441 aggcccgtga cgctagtttc aatattgtag ttgtcggtct ggatcagcac gtaaccctga
     1501 tggccggcaa tcatcgggcc aaccacgatc cccgcaatca gcaacacaaa gagcaataac
     1561 acttttagca tggttattct ccttgcggcg cggctgccgg agcatcagct tgcggtgcag
     1621 gtgcaggtgc aggtgcaggt gcaggtgcag gtttagcttc cgttgtcccc gctgccggtt
     1681 gtgccagcag gttacgcacg cgagtttgca tcagtttttc cagcatcgcc tggctttgca
     1741 gggtttccgg aagatccatc gagatatttt gctggcttaa ctggtccacc tcgtcgagga
     1801 acgctttggt ggtggcatca tcagtatcgt agtaagcacg tacccaggtg gagacgttct
     1861 ccagcgcctg gcgataagtc tcttcctggt gacgcggtac agcttgtgct gcgaccagca
     1921 ggcgagagcg aatattttcg cgcagataga tatcctgatt tggcgctaac agcggtacgg
     1981 cggtgtcatc acgacggcga atcgtaatga agttgtccat aaagttctgc cagctttttt
     2041 gcagattgat acgccattcg ctgatggaac tggaaagctc ttcaccgtcg gaatccatcg
     2101 gcgaaccatc gctgtcatta tcagccagac gcagattatc tacctgattt gaaagctgat
     2161 taagcttaag gatgataccg tcataatcca cctgcgatac cgcagaaagg ctggcgatat
     2221 catcggtaat tgcccgacga acggtaatca gactcgggtc attcatatcc gccaggctgg
     2281 catctgcact tttcagcaac gctgcagcgg tcgtgacgtc ttgatcgctc cacagcttcc
     2341 gtcctgcgag tttcaccagg aaatcagcct gagccagcag ccaggtttta gcatcgctgc
     2401 cggaaatagt cgcgaccttt tgttggactt catccagctg ttttgccagc gtttcttgct
     2461 gacgattcgc ctgctcaagc tgtacagctt gttgcttgat aatgccttcc agctcagctt
     2521 tttggctctc ctgggctttt tgcagtgccg taagttggtt agccagggca tcgctggtgg
     2581 cagtctgatt gacggcctgt tgtttacccc agccatacaa accgacgccc gccgccagag
     2641 caatagcgat agccaccgcg ctgagaatca atgcggtatt gttcttactc tttttttctg
     2701 ttgcgacagg ttgtgacgtg gtgtccacgg cctccctggt ctcttcaacc acggcggagg
     2761 ttttttcttg ttccgtcatt atggcttcct gttatgagag ttattgtaat gcccgtaaaa
     2821 gcgcatcgtt gtcagcgtta tcggcgacct taatgtcttg ccagcccagt tcccgggcga
     2881 gtttcgccaa acgctcactg acgaccaata gtcgacagcg tagtaaccag tgctcacgat
     2941 accattgtgg gatcagcgac cagagttgct gcaacatttc accgctggta acaacgaccg
     3001 tcgtcacctc gcgggattgc cagcgcatcg cttcttctgc accatcgtaa tggattacgc
     3061 atcgttgata acattcacaa aaagtgacct cagcaccgcg cgccgtcagg gtatccccaa
     3121 ttagctcacg accgccattg ccacgtaata tcagcgcacg tttgcccgca atattttgta
     3181 attcaggtaa ttgtagcaag acttcgctga tttcccgatc ctgcgggtag agaatcttct
     3241 gtccgcttac ggtatgtagt gccagtgcgg tggtgcgtcc aatggcgaaa taagtaggta
     3301 gtcggggcca tttacgatct tgctgatgca gctgtgattg ggcaaaagca accgcgtgtt
     3361 gcgagagggc aaacaacaga tcgctctccc ccagcgccgc cagttgatca gcaagttgtg
     3421 gtaattgtcg acccggagaa aactcaatca gtggaaaatg ccaggccacc tgccccagtg
     3481 tgcgcagacg gctcactaac tcttctccag cgggagacgg gcgggtgaca aggatactca
     3541 tgccggggcg tctccattat agacttcagc gaggatctcg cgcgcgccgt tattcagtag
     3601 ctcttctgcc agcgaaatcc ccatttgttc ggcatcttgc ggcgcaccgc ggcgttcacc
     3661 gcgaataatc tgcgaaccgt ccggcgcgcc gaccagcgca cgcagccaga tttcaccatc
     3721 aataagctcg gcgtagctac caattggcac ctgacatccg ccttcgagac gggtattcat
     3781 ggcgcgttct gcggtaacgc gcagtgcagt ttcgtggtga ttcagcgcgg caagcagctc
     3841 gcgagtgcgt gtatcatcaa ggcggcattc aataccgacc gcaccttgtc ctaccgccgg
     3901 aagagaaatc tcgggtggta acgcggcgcg aatacgtgac tccagaccta aacgttttag
     3961 tccggctacg gcaagaatga tggcatcgta ttcgccgtta tccagtttac tcaggcgagt
     4021 gccgacgttg ccgcgcaggg agcggataat cagatccggg cggcgttcag ccagttggca
     4081 ctggcgacgt aaactggacg tcccgacgat actgcctgcc ggtaacgcat ccagactttc
     4141 atagttattg gacacaaagg catcgcgagg atcttcacgc tcgcaaatgg tgaccagccc
     4201 cagaccttgc gggaattcaa ccggcacatc tttcattgaa tgtacggcga tatcggcgcg
     4261 attctccagg agcgcgactt ccagctcttt aacaaataag ccttttccgc ctactttcgc
     4321 cagcggcgta tcaagaatca catcgccgcg cgtcaccatc ggtaccagtt caacgaccag
     4381 gcccggatgg ctcgccatca acttgtcttt gacatagtgt gcctgccaga gtgcaagtgg
     4441 gctttggcgt gtggcaattc ttaaaacatt gtctaacatg cttgttaccg tcattatcat
     4501 ccgtggtcca tcctaacatc cttgccagag cgatgtcagt gttgtggtga aacgtagacg
     4561 cctgcgcaaa ccgtaaaatg aggtctggca gtggatcctg acaggcgttt cacgccgtcg
     4621 taataaggaa tttacagaga ataaacggtg ctacacttgt atgtagcgca tctttcttta
     4681 cggtcaatca gcaaggtgtt aaattgatca cgttttagac cattttttcg tcgtgaaact
     4741 aaaaaaacca ggcgcgaaaa gtggtaacgg ttacctttga catacgaaat atcccgaatg
     4801 ccgcgtgtta ccgttgatgt tggcggaatc acagtcatga cgggtagcaa atcaggcgat
     4861 acgtcttgta cctctatatt gagactctga aacagagact ggatgccata aatcaattgc
     4921 gtgtggatcg cgcgcttgct gctatggggc ctgcattcca acaggtctac agtctactgc
     4981 cgacattgtt gcactatcac catccgctaa tgccgggtta ccttgatggt aacgttccca
     5041 aaggcatttg cctttacacg cctgatgaaa ctcaacgcca ctacctgaac gagcttgaac
     5101 tgtatcgtgg aatgtcagta caggatccgc cgaaaggtga gcttccaatt actggtgtat
     5161 acaccatggg cagcacctcg tccgtagggc aaagttgttc ctctgacctg gatatctggg
     5221 tctgtcatca atcctggctc gatagcgaag agcgccagtt actacaacgt aaatgcagcc
     5281 tgctggaaag ctgggccgcc tcgctgggcg tggaagttag cttcttcctg attgatgaaa
     5341 atcgcttccg tcataatgaa agcggcagcc tggggggcga agattgtggc tccacccagc
     5401 atatactgct gcttgacgaa ttttaccgta ccgccgtgcg tctcgctggt aagcgtattc
     5461 tgtggaatat ggtgccgtgc gacgaagaag agcattacga cgactatgtg atgacgcttt
     5521 acgcgcaggg cgtgctgacg ccaaatgaat ggctggatct cggtggctta agctcgcttt
     5581 ctgctgaaga gtactttggt gccagccttt ggcagctcta caagagtatc gattccccat
     5641 acaaagcggt gctgaaaaca ctgctgctgg aagcctattc ctgggaatac ccgaacccac
     5701 gtctgctggc gaaagatatc aaacagcgtt tgcacgacgg cgagattgtg tcgtttggcc
     5761 tcgatcctta ctgcatgatg ctggagcgtg ttactgaata cctgacggcg attgaagatt
     5821 tcactcgtct ggatttagta cgccgctgct tctatttaaa agtgtgcgaa aaactcagcc
     5881 gtgaacgcgc ctgcgtaggc tggcgtcgtg aagtgctaac ccagttagtg aaagagtggg
     5941 aatgggacga tgctcgtctg gcaatgctcg ataaccgcgc taactggaag attgatcagg
     6001 tacgtgaggc gcacaacgag ttgctcgacg cgatgatgca gagctaccgt aatctgattc
     6061 gctttgcgcg ccgcaataac cttagcgtct ccgccagccc acaggatatt ggcgtactga
     6121 cgcgtaaact gtatgccgcg tttgaagcgt taccgggtaa agtgacgctg gtaaacccgc
     6181 agatttcgcc cgatctctcg gaaccgaatc tgacctttat ttatgtgccg ccgggccggg
     6241 ctaaccgttc aggttggtat ctgtataacc gcgcgccaaa tattgagtcg atcatcagcc
     6301 atcagccgct ggaatataac cgttacctga ataaactggt ggcgtgggca tggtttaacg
     6361 gcctgctgac ctcgcgtacc cgcttgtata ttaaaggcaa cggcattgtc gatttgccta
     6421 agttgcagga gatggtcgcc gatgtgtcac accatttccc gctgcgcctg cctgcaccga
     6481 cgccaaaagc gctctacagc ccgtgtgaaa ttcgccatct ggcgattatc gttaacctgg
     6541 aatatgaccc gacagctgcg ttctgcaatc aggtggtgca ttttgatttc cgtaagctgg
     6601 acgtcttcag ctttggcgag aatcaaaatt gcctggtagg tagcgtggac ctgctgtacc
     6661 gcaactcgtg gaacgaagtg cgtacgctgc acttcaacgg cgagcagtcg atgatcgaag
     6721 ccctgaaaac cattctcggt aaaatgcatc aggacgccgc accgccagat agcgtggaag
     6781 tcttctgcta cagccagcat ctgcgcggat taatccgtac tcgcgtgcag caactggttt
     6841 ctgagtgcat tgaactgcgt ctttccagca cccgccagga aaccgggcgc ttcaaggcgc
     6901 tgcgcgtttc tggtcaaacc tggggcttat tcttcgaacg cctgaatgta tcggtacaga
     6961 aactagaaaa tgccatcgag ttttatggcg ctatttcgca taacaaactg cacggcctgt
     7021 cagtgcaggt tgaaaccaat cacgtcaaat taccggcggt ggtggacggc tttgccagcg
     7081 aagggatcat ccagttcttc ttcgaagaaa cgcaagacga gaatggcttt aatatctaca
     7141 ttctcgacga aagcaaccgg gttgaggtgt atcaccactg cgaaggcagc aaagaggagc
     7201 tggtgcgtga cgtcagtcgc ttctactcgt catcgcacga ccgttttacc tacggctcaa
     7261 gcttcatcaa cttcaacctg ccgcagttct atcagattgt gaaggttgat ggtcgtgaac
     7321 aggtgattcc gttccgcacc aaatcgattg gcaacatgcc gcctgccaat caggatcacg
     7381 acacgccgct attacagcag tatttttcgt gatgagcgtg ccggaaagcg aggcttatcc
     7441 ggcatgcaat cttagcggaa actgactgtt tcacccgctt gctgcgttgc cgcctgttcc
     7501 agcaaatccc agaaggtttc gccgctacga tcgcaaatcc actcatcgcc tttcagatca
     7561 aaatggtagc cgccctgttt ggttgccagc catacctggt gcagcggttc ctggcggttg
     7621 ataatgattt tgctcccgtt ttcaaaggta atggtcagta caccgccgtt gatttcgcag
     7681 tcgatatcgc tatcgccatc ccagtcgtcc agacgctctt caatggtcag ccacagttga
     7741 tcagccaggc gatgaaattc actgtcgttc attgttgtat cctgttttta agtgatggcg
     7801 gcagtatagc ggcatggggt cagagcttca aagtttgctc aatgtctggt gttttgtggc
     7861 actcttcctc cgtcgtcaag atgatgatgt catcattgga tgaaaacatg ttctgatcca
     7921 gattacgtgt cctggccgga cagggcgatg cggttcgata aagttggcta ccgatccagg
     7981 tataaacata gcgattgtca taagcgaaac gttcggtggt tttcaatggt ccatcaatag
     8041 caaaaatttg aatatcatcg cgtaaacggg aagagcgcat aaagtaacca tggcctttcc
     8101 cggcgttaat ccgttgtttc aggaaaggtg cagaagagtc gagcgttatt atttccaggg
     8161 tgtcttcacc aaaaggagac aatttcgcaa tgtacagatg atttttgtac tgcgcgacac
     8221 gatcggtgat gggatgaaat gctttcgggt caatatcggg gagtttctcc cattgactcc
     8281 attgctggtc tgtagagcga agcttacgcc agctaacatc ggtttcgccg acgagaaaag
     8341 agccttcgcc atcagaatat tgagcaatgt taacggatga gttatagggg attcccgttg
     8401 gaataaacgc accatcctca agaagtctgc cgtcaagctc accactacgg tagtttgccc
     8461 aatagccata tcctgaatct ttggttttcc atttcacccg cacggctttg gtattgggag
     8521 ttgggtagcg ccaaagctgt gagccatcga atgcgtaaac ataacgatcg tcgtgtgcat
     8581 accatttctt gtaatggggg ctatcgctaa ggtgagcctc atgctcgtca ggaacacgat
     8641 aatccatcag gataagcggc ccggaagaat cgaatacctg gaggccatct tcttcttcac
     8701 catcagctgg taatttggtc aataaatagc cgtgagttct tcccgcgtta aagcgtttat
     8761 ctatcaccaa accaggatta tccagtgtga cgatatttag cctattttcg ccaaatcccg
     8821 tttttttgat ggtgtacagc ttgtcctgat actgagcaac ggtattacta agaggatgaa
     8881 attgttctgg atcgaggccg ggtaggactt cttgctggca ttctggccct ttgcgccatg
     8941 aaacagagtg ttcctgcaaa gtgaacgccg cgcagctatt tgccctatct ttgtccagat
     9001 ttccctgatt cagcaccttc cggtgcaggc cgcttttatc tcgccaggtt aataacgaac
     9061 cgggcatcca gcgtacgaca gtaaatgtct ctgggtcggc tttaagctgt tcgccattga
     9121 ttatgatttt ggtccgtgta cgggctaacg tatcccaggg gccaaatggt acggcaacgc
     9181 aggggttaaa cgtggtggaa aactttcctc gctgatccca ggacttctgc gccagcacac
     9241 ggaaactctc ggggtcagcg aggcgatgtc catcgacata taagaaatta gcgtcacgta
     9301 atatcagccc cagaaagtca ggtttccagg gggaacggaa tttcacctga tgcagcgttt
     9361 taatatctag actattttct ccgccattgt catcggtgcg cgtgccttca aaatagagac
     9421 tgtgtttatc tgcggcaaaa tcaccccatg ccttgaacga ggcgacatcg acgggaggtt
     9481 tgtctggcgg gttttgcaca acttcacccg cgtagaggat atagcgacca tcgctatgcc
     9541 aggagtaatg aggataccag gcctgtgcgc cttcatagag gtagtcggtt gtcagatcag
     9601 tcagaggagg tactttatcc catttgtcta gtgttgtcgg gtctattaat aaattgggta
     9661 aacggcgcaa acttttgtaa ttcacgtcag gaagagaaag atttgtttct ttataagggc
     9721 gcaggtatac cacgcctttt ccctgtttct ggtaaatatt atgttcaggc gcggaaagcc
     9781 gacatcccca tgccgacgat aaaaacaacg aacaagacaa caaacaaacc acattgcgaa
     9841 agtgcataaa gccatcctgg cgcgaggtgt tgatcacgaa actgctagca gaacataaat
     9901 cctcacaagt aagcgtaact ctcgcagcaa atcgtcactt ttcagaatcc ataaaggtca
     9961 tcgctatttt ctgatagttg ctggaataaa cgtgatattt tcattcacga tataaacaca
    10021 atcccggaac tgccccatag tacggctaat agcataaaaa tattccggat tgacccctgg
    10081 aagggaggca atgtgggatt ccgccccccg ttgccttctt tattgtcttt cacgagttgt
    10141 acccaggttc caccacgggt atctaacatt cgccacatta ttacgctttc tgccgaacgt
    10201 gatttttata gaaatctatc aattcatttt ccctgtacca taaagtttat ccttcacatc
    10261 ctgccgaacg cgggtcttgt accccccgtt gtgaagatct tcatacggag aaaaagaatc
    10321 tttactccac cccagcaaca ctttatcgcc aggcataaaa tgcacgtgca ggtaattttc
    10381 tccccattct ctttttggga gaggtaacgt catattgcgt ttctccagac gcattccagc
    10441 attatactgt gtacgagtag tacttaatgt ccatataacc tctgccgtat ccccactgac
    10501 atcaccgcaa caggtgattg aaccgccacc attcgcaaaa gcattcgcgc ccgccacgcc
    10561 gttaacactg aatcccagta tcggacggtc aatttcactg tggatttcca gtatcactcc
    10621 acctcttggt cctccccata ctgcctgtcc tatattccac aacacgacca gtactggaac
    10681 gacaattaac gtccgccata cccagcgccc ccagcgggca taaccgcgcg taagcttttg
    10741 gtcaaatttt tccagttggg taaatagccc catcgatttt attccctctt ctcacctggt
    10801 tggcaaagtt gcacgtcact ggcgactaag gtcagacaac gaaatttcgt ggtacgccac
    10861 tctcttaaaa gtcccatgca ggcgataaga acaagacaac aaacaaacca cattgcgata
    10921 gtgcataaag ccatcctggc gcgaggtgcc gatcacgaaa ctaccagcaa aacataaatc
    10981 gccacgtgta agcgttatac tcgcagcatt tcctcacttt tcagacttca taaagagtcg
    11041 ctaaacgctt gcttttacgc cttctcctgc gatgatagaa agcagaaagc gatgaacttt
    11101 acaggcaatc cataatgaaa aacgtgttta aggcactcac tgtattactt actctcttca
    11161 gcctgacggg ctgcggtctg aaaggtccgc tctatttccc gcctgcagat aaaaacgcac
    11221 cgccgccgac caaaccggta gagatgcaaa cgcaatccac ggtgccggat aaaaacgacc
    11281 gcgccactgg cgatggtcca tcccaggtga attactaaaa gtcagtttct gtacccgcgt
    11341 gattggagta aatgatgcag ttctcgaaaa tgcatggcct tggcaacgat tttatggtcg
    11401 tcgacgcggt aacgcagaat gtcttttttt caccggagct gattcgtcgc ctggcggatc
    11461 ggcacctggg ggtagggttt gaccagctgc tggtggttga gccgccgtat gatcctgaac
    11521 tggattttca ctatcgcatt ttcaatgctg atggcagtga agtggcgcag tgcggcaacg
    11581 gtgcgcgctg ctttgcccgt tttgtgcgcc tgaaagggct gactaataag cgcgatatcc
    11641 gcgtcagcac cgccaacggg cggatggttc tgaccgtcac cgatgatgat ctggtccgcg
    11701 taaatatggg cgaacccaac ttcgaacctt ccgccgtgcc gtttcgcgct aacaaagcgg
    11761 aaaagaccta tattatgcgc gccgccgagc agacaatctt atgcggcgtg gtgtcgatgg
    11821 gaaatccgca ttgcgtgatt caggtcgatg atgtcgatac cgcggcggta gaaacgcttg
    11881 gtcctgttct ggaaagccac gaacgtttcc cggagcgcgc caatatcggt tttatgcaag
    11941 tggttaagcg cgagcatatt cgtttacgcg tttatgagcg tggggcagga gaaacccagg
    12001 cctgcggcag cggcgcgtgt gcggcggttg cagtagggat tcagcaaggt ttgctggccg
    12061 aagaagtacg cgtggaactc cccggcggtc gtcttgatat cgcctggaaa ggtccgggtc
    12121 acccgttata tatgactggc ccggcggtac atgtctacga cggatttatt catctatgaa
    12181 gcaaccaggg gaagaactgc aggaaacact cacggagctt gatgaccggg cggttgtcga
    12241 ttatctgatt aaaaatcctg agttttttat ccgcaatgcg cgcgcagtag aagcgatacg
    12301 tgtgccgcat ccggtacgcg gcaccgtttc gttggtcgag tggcacatgg cccgcgcacg
    12361 taatcacatt catgttctgg aagagaacat ggcgttgttg atggagcagg ctatcgccaa
    12421 cgaaggcctg ttttatcgct tactctactt gcagcgtagt ctcaccgccg ccagcagtct
    12481 cgacgatatg ctgatgcgct ttcaccgctg ggcgcgcgat ctcggcctgg cgggtgcgag
    12541 cctgcgcctg ttcccggatt gctggcgttt aggtgcgccg tcgaaccaca ctcatctggc
    12601 attaagccgt cagtctttcg aaccgctgcg tattcagcgt ttggggcagg aacagcacta
    12661 tcttgggccg cttaacggac cggagctgct ggtggtgcta ccggaagcga aagcggtggg
    12721 atcggtggcg atgtcgatgc tgggaagcga tgctgatttg ggtgtcgtgc tgtttaccag
    12781 tcgcgatgcc agtcactatc aacaagggca gggaacgcag ttacttcatg aaattgcgct
    12841 gatgttgccg gagcttctgg agcgttggat tgaacgcgta tgaccgattt acacaccgat
    12901 gtagaacgct acctacgtta tctgagcgtg gagcgtcagc ttagcccgat aaccctactt
    12961 aactatcagc gtcagcttga ggcgatcatc aattttgcca gtgaaaacgg cctgcaaagc
    13021 tggcagcaat gtgatgtgac gatggtgcgc aatttcgctg tacgcagccg ccgtaaaggg
    13081 ctgggagctg caagtctggc gttacggctt tctgcgctac gtagcttttt tgactggctg
    13141 gtcagccaga acgaactcaa agccaacccg gcgaaaggtg tttcggcacc gaaagcgccg
    13201 cgtcatctgc cgaaaaacat cgacgtcgat gatatgaatc ggctgctgga tattgatatc
    13261 aacgatcccc tcgccgtgcg cgatcgggca atgctggaag tgatgtacgg cgcgggtctg
    13321 cgtctttccg aactggtggg gctcgatatc aaacacctcg acctggagtc cggcgaagtg
    13381 tgggtgatgg gaaaaggcag caaagagcgc cgcctgccga ttggtcgcaa cgctgtggcg
    13441 tggattgagc actggcttga tttgcgcgac ctgtttggta gcgaagacga cgcgcttttt
    13501 ctgtcgaaac tgggcaagcg tatctccgcg cgtaatgtgc agaaacgctt tgccgaatgg
    13561 ggcatcaaac aagggctgaa taatcacgtt cacccgcata aattacgtca ctcgttcgct
    13621 acgcatatgc tggagtcgag cggcgatctt cgtggtgtgc aggagctgct gggtcatgcc
    13681 aacctctcca ccacgcaaat ctatactcat cttgattttc aacaccttgc ctcggtgtac
    13741 gatgcggcgc atccacgcgc caaacggggg aaataatgcg tttttaccgg cctttggggc
    13801 gcatctcggc gctcaccttt gacctggatg atacccttta cgataaccgt ccggtgattt
    13861 tgcgcaccga gcgagaggcg cttacctttg tgcaaaatta tcatccggcg ctgcgcagct
    13921 tccagaatga agatctgcaa cgcctgcgcc aggcggtacg ggaagcggaa cccgagattt
    13981 atcacgacgt gacgcgctgg cgttttcgtt cgattgaaca agccatgctc gacgccgggc
    14041 tgagtgccga agaagccagt gcaggcgcac acgcagcaat gatcaacttt gccaaatggc
    14101 gcagccgaat cgatgtcccg cagcaaaccc acgacacctt aaaacagctg gcgaagaaat
    14161 ggccgctggt ggcgatcacc aacggtaacg cccagccgga gctgtttggt ttaggggatt
    14221 attttgagtt tgtgttgcgc gctggcccgc acgggcgctc aaaaccgttc agcgatatgt
    14281 acttcctggc ggcggaaaaa ctcaacgtgc cgatcggcga gatcttacat gttggggacg
    14341 atctcaccac tgacgtgggt ggggcaattc gcagcggaat gcaggcctgt tggatcagac
    14401 cggaaaatgg cgatctgatg caaacctggg acagccgttt actgccgcat ctggaaattt
    14461 cccggttggc atctctgacc tcgctgatat aatcagcaaa tctgtatata tacccagctt
    14521 tttggcggag ggcgttgcgc ttctccgccc aacctatttt tacgcggcgg tgccaatgga
    14581 cgtttcttac ctgctcgaca gccttaatga caaacagcgc gaagcggtgg ccgcgccacg
    14641 cagcaacctt ctggtgctgg cgggcgcggg cagtggtaag acgcgcgtac tggtgcatcg
    14701 tatcgcctgg ctgatgagcg tggaaaactg ctcgccatac tcgattatgg cggtgacgtt
    14761 taccaacaaa gcggcggcgg agatgcgtca tcgtatcggg caactgatgg gcaccagcca
    14821 gggcggcatg tgggtcggca ccttccacgg gctggcgcac cgtctgctgc gtgcgcacca
    14881 tatggacgcc aatctgccgc aggattttca gatcctcgac agcgaggacc agctacgcct
    14941 gctcaaacgc ctaatcaagg cgatgaacct cgacgagaag cagtggccgc cgcgccaggc
    15001 aatgtggtac atcaacagcc agaaagatga aggcctgcgt ccacatcata ttcaaagcta
    15061 cggtaatccg gtggagcaga cctggcagaa ggtgtatcag gcgtatcagg aagcgtgtga
    15121 ccgcgcgggc ctggtggact tcgccgagct gctgctacgt gctcacgagt tgtggcttaa
    15181 caagccgcat atccttcagc actatcgcga acggtttacc aatatcctgg tggatgaatt
    15241 ccaggatacc aacaacattc agtatgcgtg gatccgcctg ctggcgggcg ataccggcaa
    15301 agtgatgatc gttggcgatg acgaccagtc aatctacggc tggcgtgggg cgcaggtcga
    15361 gaatattcag cgtttcctca atgatttccc cggtgctgaa actatccgtc tggagcagaa
    15421 ctaccgctct accagcaata ttctgagcgc cgctaacgcc ctgattgaaa acaataacgg
    15481 gcgtctgggt aaaaaactgt ggaccgatgg cgcggacggt gagcctattt ccctctattg
    15541 cgcttttaac gaactcgacg aagcgcgttt tgtggttaac cgtatcaaga cctggcagga
    15601 caatggcggg gcgcttgccg agtgcgccat tctctaccgc agcaacgccc agtcgcgtgt
    15661 actggaagaa gcactgttgc aggcgagtat gccgtaccgt atttacggcg ggatgcgctt
    15721 cttcgaacgc caggaaatca aagatgcgct ctcgtatctg cgcctgattg ccaaccgtaa
    15781 tgacgacgcg gcgtttgagc gcgtggtgaa tacaccaacg cggggtattg gtgaccggac
    15841 gctggacgtg gtacgtcaga catcgcgcga tcgccagtta acactctggc aggcatgtcg
    15901 tgaactgttg caggaaaaag ccctcgccgg acgtgctgcc agtgccttac agcggtttat
    15961 ggaactgatc gacgccttag cgcaggaaac tgccgatatg ccgctgcatg tacagactga
    16021 ccgggtaatt aaagactccg gcctgcgcac aatgtacgag caggagaaag gcgaaaaagg
    16081 ccagacgcgt atcgaaaact tagaggaact ggtaacggcg acgcgccagt tcagctacaa
    16141 cgaagaagac gaagatttaa tgccgctgca ggcgttcctc tcccatgcgg cactggaagc
    16201 gggcgagggg caggcggata cctggcagga tgcggtgcag ttgatgacgc tacactcggc
    16261 gaaaggtctg gaattcccgc aggtgtttat cgttggtatg gaagagggca tgttcccaag
    16321 ccagatgtcg ctggatgaag gcgggcgtct ggaagaagaa cgccgtctgg cctacgttgg
    16381 cgtaacccgc gcgatgcaga aactgacgct gacctacgcg gaaacccgcc gtctatatgg
    16441 caaagaggtt taccatcgcc cgtcgcgctt tatcggtgag ttgccggaag agtgtgtgga
    16501 agaggtgcgc ctgcgcgcca cggtaagccg cccggtcagc catcaacgga tggggacgcc
    16561 gatggtcgag aacgacagcg gctacaagct cggccagcgc gtacgccacg ctaagtttgg
    16621 tgaaggcacc atcgtcaata tggaaggcag cggtgagcat agccgtttgc aggtggcgtt
    16681 ccagggccag ggtattaaat ggctggtggc ggcttacgcc cggttggaga cggtgtgacg
    16741 ttgcgggatg cggtgcgcca caccgcattc cgcatcagca ccttacttag cgcacttctt
    16801 cacatcaata ttccagtcag caccagacgc aacctgatgt ttctgcgcaa aattatccat
    16861 attcagcgcc acggaaacat tcaataagct gttcagatac accatcggct ggccttccgg
    16921 cacatcgccg aagcttgcga catacggcat tttccctgcg tatttttgct gggaaccttc
    16981 ggaaatggtc acacacaacg tgtcgttgag tttgattcct gcctgtttca gcaactcgtc
    17041 gctgatattg ctccagacgt tgccgtactg aatatcgaga atcgggatat tgcccttcac
    17101 ttcacctttt gttgcttttg ctttctgata agaaagttcg acaacttttg caggaagctc
    17161 tggccccacc tgttcgaacg taatcgctcc ggaagccaga cgcgcaccag tgtaggcata
    17221 tacatcgcga ccatggaagg tatatgactt ttcagaacct ttcaggcggt tagctttttc
    17281 gtcgatttcc cggacgctat caatacccag agtttgtgcc accaaagtga gcgtaccgtt
    17341 atctggtgaa acgaagtatt gaccgttttt ggttttcagc actaccgatt tacggtcagt
    17401 gcctacgccc ggatcgacca cgctaacaaa caccgaacct tgcggccagt aactggcagt
    17461 ctgatacagg cgataagcgc cttcccagat gttatacggt ggaatttcgt gtgttaaatc
    17521 aaagatttta agattatggt caacgccgaa tgcgacccct ttcatcgccg agacggcacc
    17581 atctttcagg ctaaaatccg tttgtaaaat caaagcgtta tccgcataag cggatgatga
    17641 ccccagacag gttaataaaa ataatgttgc gagcgttttt ttcatttttg tttcccttca
    17701 tttatctgat gattttccag ctgtgtagcc agccccagtt tgtctggcct cctcgagtgt
    17761 acaatgttta acctttctgc tcgacggaaa tcatggtgac aaaaggatag cgttgccacg
    17821 ggattgcctc gcctttcata tacatatgtg aaattgtgcc atcaagataa agcagctgcc
    17881 caacgttcag tttcgctttg gcataacagg caaaatcata aaaatttgtc gcctgctggc
    17941 tcaacaaaaa cacggcgttc ccatgtttat taatcccaac accgttacga attttacgtg
    18001 aggcgacgtt gggatgaata cgcggattaa ttacaccgtt ttccatcaac aatggccctg
    18061 actgcaccgc aaactgaatc tctttactgg ttttaaaggc atccagacga acgatgccga
    18121 ctttatctcc cgcgacataa aacactccgc caggacgaat aaagaaattc ccttcacctg
    18181 aagcgagatt taacgccacc ttctgctgac cgttttcgat gtacaaaccg agcggcgcat
    18241 agctttcatc atagatgccg ccgttcatcg ccatctgcac ctgaccctga ctattaatat
    18301 ccgccagcag agcatgtaac gttccccacg cttcgccatt ggctttttgc cagtacattt
    18361 tcacccgctc tgtttgagga ttaacggtat acgcctgtac ggtcagcgtc ggatctgagg
    18421 gtgcgcaatc atcagcggca acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc
    18481 gtttgagatt caaggtgatc attcctttac caatgaatag ccgatgcgcc attataggcc
    18541 ctggatgtgg gattttttta tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt
    18601 tcaactattg ttctgtggtg ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca
    18661 tgcgcgcacg atcactctaa gaggacattc gccttggaca cacccagtag atactggctc
    18721 actatcctgt catccaggat caactcctaa ggctatccct ttttgctgat agccttagcg
    18781 gttgtcagcg acctcaattt ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac
    18841 ccaatttgtt tctgtgtgcc caccgaactg tccgatattt taagcattgg gagtcccggt
    18901 catgctgagc gcatttcaac tggaaaataa ccgactgacc cggctggaag tcgaagagtc
    18961 acaacccctt gtaaatgcag tatggattga tcttgtcgaa ccggacgacg acgagcgact
    19021 gcgcgtacaa tctgaacttg gtcagagcct ggcaacccgc ccggaactgg aagacatcga
    19081 agcatcggca cgtttctttg aagacgatga cggcctgcat attcactcct tcttcttctt
    19141 tgaagatgcg gaagatcacg ccggtaactc tactgtggca tttaccatcc gtgatggtcg
    19201 tctgtttact ctgcgtgagc gtgaactgcc tgcattccgt ctgtatcgta tgcgcgcccg
    19261 tagccagtca atggtggacg gtaacgccta cgagctgctg ttggatctgt tcgaaaccaa
    19321 aatcgaacag ttggcggatg aaattgaaaa catctacagc gatctggagc agttgagccg
    19381 ggtgattatg gaagggcatc agggcgatga gtacgacgag gcgctctcca cgctggcgga
    19441 actggaagat atcggctgga aagttcgcct gtgtctgatg gatacccagc gtgcgctcaa
    19501 cttcctggta cgtaaagcac gcttaccggg tgggcaactg gagcaggcgc gtgaaatcct
    19561 gcgagatatc gaatccctgc tgccgcataa cgaatccctg ttccagaagg tgaacttcct
    19621 gatgcaggcg gcgatgggct ttatcaatat cgagcagaac cgcatcatca aaatcttctc
    19681 ggtggtatcc gtggtgttcc tgccaccgac gctggtcgct tccagctacg ggatgaactt
    19741 tgagtttatg ccggaactga agtggagctt cggctaccca ggcgcgatta tctttatgat
    19801 cctcgcgggc ctggcaccgt atctgtactt taagcggaag aactggttgt aatataaatg
    19861 ccgggtaaag attgttcttt atccggctta ttgctgaaaa ttgtcttttt caaagtcatg
    19921 cgggttgaac actcgctcta tcttattttc ctgggttagt tgatactcat tgttatcagc
    19981 aataaaaaaa tgagtattac ggtaaggata ttcattaata accattaaat agttttttac
    20041 gatgcaatta tcattgctct taatcgtcgg gaaacagtag agatcttcat acggcccttc
    20101 gatatatttt ttatagtcag tgaagccatt accgatatca ataacatacc aggtatgcca
    20161 actaccttct ggtaggggat gttgattttt aatttcattc tgatgcttca gataccaatt
    20221 tattttatcg ctgtcagtcc atggcagatg atcaacgacc agaaagactg aattgccagc
    20281 atcagagtat gtatacaaaa tctgtacagg acgcaatgtc cataaattat agatgcagca
    20341 tagaagaaat aaacataata taattttctg gagtttgctt ttcatagata attttcgatt
    20401 ctttctatag tgttaaaatt ggtgaaaaaa ggtttgaaag caaagtcttt atgccgttgt
    20461 aaaaagaacc atatgcgaaa aaatctatat ttgctataag tcttgttttt gatatcagtt
    20521 acatcaagac cgaagtggtc ctgtgctaca aaatgaattg ttgcactcca tcctatagcg
    20581 tatttttgaa aacttaaaag agatattttt tgagctgaaa tatcatgaac actgattcct
    20641 aagccattaa aactgtcctc gaagtcatta aatttattta gtcttgatga aagtaattcg
    20701 gtcttaatct tttgtaggag aagaggccgg tatcctatcg taggtgttga agaaatatca
    20761 ttttcgatac attctttgat tacacgtatt gggctattat aatcatattt gtttattcgt
    20821 tcatgaaaag ataagttcaa ttcctggcta tggaaactgt tgccatttcc atatctaaaa
    20881 tgatcaatca gatcctggat gagtgttttg tattgtccaa aaaaggaaaa catttttgcc
    20941 agatctttca tttctgcaaa taaaatatca atacattcac tttgtgatat ttttgttcca
    21001 gaagttggaa cactaaaagc accataagta gaattcggcc caggaaaatg gtatttaatg
    21061 aggcggtaag gatcaacttt tgcagagatg tcgctcaagc cgagtgagag aaaatcttgt
    21121 tcttccatat ccccatattg catatcatca gcagaaaaat catcgaataa agtaatagtt
//
LOCUS       DAZGHF010000081        21044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGHF010000081 DAZGHF010000000
VERSION     DAZGHF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.081"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(14..1474)
                     /gene="ydfI"
                     /locus_tag="ACHK3A_003129"
     CDS             complement(14..1474)
                     /gene="ydfI"
                     /locus_tag="ACHK3A_003129"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO8741180.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAVMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1564..2931)
                     /gene="ydfJ"
                     /locus_tag="ACHK3A_003130"
     CDS             complement(1564..2931)
                     /gene="ydfJ"
                     /locus_tag="ACHK3A_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO8741181.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVTAAQ
                     PSRSL"
     gene            complement(2989..4008)
                     /gene="rspB"
                     /locus_tag="ACHK3A_003131"
     CDS             complement(2989..4008)
                     /gene="rspB"
                     /locus_tag="ACHK3A_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO8741182.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(4020..5234)
                     /gene="rspA"
                     /locus_tag="ACHK3A_003132"
     CDS             complement(4020..5234)
                     /gene="rspA"
                     /locus_tag="ACHK3A_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO8741183.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(5440..5766)
                     /gene="ynfA"
                     /locus_tag="ACHK3A_003133"
     CDS             complement(5440..5766)
                     /gene="ynfA"
                     /locus_tag="ACHK3A_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO8741184.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            5901..6242
                     /gene="ynfB"
                     /locus_tag="ACHK3A_003134"
     CDS             5901..6242
                     /gene="ynfB"
                     /locus_tag="ACHK3A_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO8741185.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            6277..6837
                     /gene="speG"
                     /locus_tag="ACHK3A_003135"
     CDS             6277..6837
                     /gene="speG"
                     /locus_tag="ACHK3A_003135"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO8741186.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(6840..7550)
                     /gene="ynfC"
                     /locus_tag="ACHK3A_003136"
     CDS             complement(6840..7550)
                     /gene="ynfC"
                     /locus_tag="ACHK3A_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO8741187.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            7658..7963
                     /gene="ynfD"
                     /locus_tag="ACHK3A_003137"
     CDS             7658..7963
                     /gene="ynfD"
                     /locus_tag="ACHK3A_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO8741188.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            8162..10588
                     /gene="ynfE"
                     /locus_tag="ACHK3A_003138"
     CDS             8162..10588
                     /gene="ynfE"
                     /locus_tag="ACHK3A_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO8741189.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLVNENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            10649..13072
                     /gene="ynfF"
                     /locus_tag="ACHK3A_003139"
     CDS             10649..13072
                     /gene="ynfF"
                     /locus_tag="ACHK3A_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO8741190.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            13083..13700
                     /gene="ynfG"
                     /locus_tag="ACHK3A_003140"
     CDS             13083..13700
                     /gene="ynfG"
                     /locus_tag="ACHK3A_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO8741191.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            13702..14556
                     /gene="ynfH"
                     /locus_tag="ACHK3A_003141"
     CDS             13702..14556
                     /gene="ynfH"
                     /locus_tag="ACHK3A_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO8741192.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAANNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWYNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCTGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            14599..15213
                     /gene="dmsD"
                     /locus_tag="ACHK3A_003142"
     CDS             14599..15213
                     /gene="dmsD"
                     /locus_tag="ACHK3A_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO8741193.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            15372..16664
                     /gene="clcB"
                     /locus_tag="ACHK3A_003143"
     CDS             15372..16664
                     /gene="clcB"
                     /locus_tag="ACHK3A_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO8741194.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(16617..17312)
                     /gene="bioD"
                     /locus_tag="ACHK3A_003144"
     CDS             complement(16617..17312)
                     /gene="bioD"
                     /locus_tag="ACHK3A_003144"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO8741195.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(17437..18657)
                     /gene="mlc"
                     /locus_tag="ACHK3A_003145"
                     /gene_synonym="dgsA"
     CDS             complement(17437..18657)
                     /gene="mlc"
                     /locus_tag="ACHK3A_003145"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO8741196.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(18792..19685)
                     /gene="ynfL"
                     /locus_tag="ACHK3A_003146"
     CDS             complement(18792..19685)
                     /gene="ynfL"
                     /locus_tag="ACHK3A_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8741197.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMMDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            19792..>21044
                     /gene="ynfM"
                     /locus_tag="ACHK3A_003147"
     CDS             19792..>21044
                     /gene="ynfM"
                     /locus_tag="ACHK3A_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8741198.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYVGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
BASE COUNT         5306 a         5430 c         5053 g         5255 t
ORIGIN      
        1 ggcattgctt tttttacttc acggaatatt tcgccacggt cgctttcgca ccatgcgcta
       61 ataaagacaa gtacgcttcc gtcacttttg cagtaaacaa gctattgtct ggcaaatcat
      121 caccaaagat cgccttaatc gccagcaatg actggacgcg cgctttccct tcggcactac
      181 tttgtacagc cttctgaata acaggtaaca gtgggtcact gatttctatc ggatttccct
      241 gttcatcaac accaccgaca taacgcatcc aacctgcgac gcccagcgcc agcagatcga
      301 acttgctgtc atgcgccaga tgccagcgaa cagaatccaa catccgctgt ggcaatttct
      361 ggctaccatc catcgcaatc tgccaggttc gatgacgtag cgccgggttg ctatagcgtt
      421 caattaatcg gttagcgtaa tcttgcaaat caacgccctg cactttcaac gtcggcgctt
      481 gttcctgcaa catcaaggca tacgctgcat gacgataatg ttcatcttcc atgcagtcat
      541 taatgtgctg atatccggca agatacccca gatacgccag gaatgaatga ctgccgttga
      601 gcatgcgcaa cttcatctct tcataaggca gcacatcact aaccagttcg gctcccgctt
      661 tttcccattc cggacgtccg gcaacaaagt tatcttctat tacccactgg cggaaaggtt
      721 cacaggcaac gcccgcagga tcgcgcacac cggtaagttg ttcgattttc gccagcgtat
      781 cctccgtcac tgcgggcaca atacggtcca ccattgttga tgggaaagtc acgttttctt
      841 cgatccattg tgccagtttt acatcaacag cttgcgcgta ggaagtgaca acgtcacgca
      901 taacatgacc gttttctggc atgttgtcac atgacatgac ggtaaatgcg ggaagtcctg
      961 ccgctttacg gcgagccagc gcctcgacaa tcacccctgt tgcagttttc ggctggtggg
     1021 gattttgtac gtcggcaacg accatcgggt gatcgagcat taactgtccg gtcgccggag
     1081 agtggaaata ccctttttcg gtgattgtca gagagacaat cgcgatttgc ggttcacaca
     1141 tcacagccaa cacggtttct aagccatcaa tctgtacgtg caaggctttt ttaacgacgc
     1201 caacgacgcg agccgtccac gcatcggccg acatttccgc aacggtataa agattatctt
     1261 gctgttgtaa atcggcaatt tgctgttcgc cgccgattaa attgacctca taatatcccc
     1321 agtcactgaa atgttccgta gcaagaatat cggcatacac accctgatgc gcacggtgaa
     1381 atgcaccaaa gcctaaatga acaattcttg gggccaggtt attacgatca taaacaggga
     1441 gcgtcgcttt tgctgataac aaattatttc ccataacaat tccttaaatt taaatatggc
     1501 aagctgcatg ttttgttata tgaataaaaa atcccctctc cggtaagaga agggatggag
     1561 gatttacaga cttcttgaag gttgcgcagc tgttacaaca cgcggttgat cttccgcggc
     1621 atcttccagt gcacttaaat cacggtcttt cacttctggc attttcagcg cagagattaa
     1681 accaatcact gaatatgcca tgatcataat ggcgatcgga taccaggatt ccgtcatggt
     1741 gcagaaaata cccgccagga taggaccaaa accggaagcg ataagaccac caatttcttt
     1801 agaaatggcc atccgggtaa agcggttttt acagccgaac atttctgcca tggtaatgtt
     1861 ttccagagca aataatccca gcaccgcaca gttatgaatc acaatcagtg ccaccataat
     1921 ggtgctcggg gcatagcttt tatctacgat gatagaaagc attggccatg ccagcacaat
     1981 cgcggaggta ttcataataa tatacgggat ccggcgacca attttatcgg ataaccaacc
     2041 aaggaacgga atggtcataa agccgagaat cgaactgatc atcaatgcat ctgttggaat
     2101 tgctttgtta aacaataacg tctgtactaa atagcctgca aggaaagtct gaattaaacc
     2161 ggagttacca gcctgaccaa aacgcagccc tgttgccagc cagaaggatt tgctctggaa
     2221 catgctacca gcaggtgcag gttttgctgt cggttggttg ctgtcgttaa ccttctcaaa
     2281 gaccgggctt tctttcagat tcatacgcaa ccagatagca aagaccatca cgacaacgct
     2341 cgccaggaac ggtatacgcc atccccacgc caacagttcc tctttactga gaatgaagaa
     2401 cataaaggcc cagattgccg ttgcgcttaa ggttccgcag ttagttccca tagccacaaa
     2461 tgaggagata attccgcgct taccttttgg cgcatattcc gccagcatcg taccggcacc
     2521 ggaaatttcc gcacctgcac ccaatccctg aataatacgc agcgtcacca gcaagatggg
     2581 tgcaaaaaca ccaatctgtg cataggtcgg taacacacca attaaggtgg tacagatccc
     2641 catcatggtg atggtaataa agagtacttt tttacgccct attctgtcgc ccattttgcc
     2701 gaaaataaat gctccgacaa tacgcgccac ataacctgca ccgtaggttc ccattgccag
     2761 aattaacgcc attgccgttg atgattcagg aaaaaatatt tcatgaaaca ctaacgctgc
     2821 accgagcgaa tataactgga aatccataaa ttcaagcgca gtgcccagcc atcccgatac
     2881 tgctgctttc accaaatcct tagtgcttct ttcgtgtttt tctattgtca taatggttat
     2941 ctctaaaaaa gaggtaagat gcgtactact tactcgccgt tattggtatt attcagaaaa
     3001 agtaagtaag actttgcagc aatgtttttg atcctgttca aataaactaa tggcatcagc
     3061 aacatgctgg aaatcaaacg tatgggtaat taatttttct ggtttaatta accctttact
     3121 taaccagtcg ataacgaccg gaaatttatt tgcatttaag cgtgaagaga aaatagagag
     3181 ttcttttccg gtaattcctt gctgaatcac ttcagacggt tcactggaga agcccatcaa
     3241 tacaatacgt gccgctggag aagccagcgt tacagcctct ttcaggatag aaggatgaca
     3301 agccgcatcg ataattaatg tcggcttgat gcctttttca gcgaaactct cgccaagcgg
     3361 tgtctggctg ttattaatcg cccagtctgc cccgctctct ttcgcttttt ccagtcgttc
     3421 atcaatgcga tcggcaacaa tcacattttt aacgttatag acgcctttta atacctgaac
     3481 gatcgtcagg ccgattggac cggcaccgta aaccagaacg gtatcatttt cagtcggttg
     3541 accatgaccg gtaacgttag ccgcaatggt aaaaggttcg atcatcaccg catattgatc
     3601 ggccactgct tcaggaattt tccacgcatt ttttgccgga accacggcat attcactgaa
     3661 accaccgtca gcgtgcacac ctaatacagc cagtgtcgta caaacgttcg gcttacctat
     3721 agagcacgga tagcaatgcc cacagctgac caccggatcg acagcaacgc gttcaccgac
     3781 tctggcgctt tccacgcctt cacccactgc atcaatgacg ccaaagaatt catgaccaat
     3841 gacgcgcgga tatttcgcaa aaggattatg cccacgataa atatggctat ctgaaccaca
     3901 aattccggca agtttcactt ttactcgtac ttcacccgct gacggggtgg gtatttcgcg
     3961 ttcgataatt gccagttgat tcggtttttc aattaaaatg cttttcatta tcttactcct
     4021 taccagttcc acagcgtgcc atcttccaga cgtgcgactg gcagataagc aggttcatag
     4081 ggatatttcg ccgccagctt ttcatcgaat tcgatgccaa gacccggttt gtctcccgga
     4141 tgcatatagc cgttatcgaa agtccagttg tgcgggaaga cttcaagcat ttgttcggaa
     4201 taacccatgt actcctggac accgaaattg gggacccaca gatcaaagtg cagcgccgca
     4261 gccatgcaga ctggcgacaa atcggaagga ccgtgtgagc cagtacgtac ctgatacagc
     4321 gaagcaaaat cggcaatccg gcgcataccg gtaatccccc ccgcgtgagt cagcgtggtg
     4381 cggatatagt cgataagttg ttcttcaatc agctgtttgc agtcccagat gctgttgaag
     4441 acttcaccca ctgcaattgg cgtgacggtg tgttggcgaa tcaggcggaa acactcctgg
     4501 ttttccgcag gcgtcgggtc ttccatccag aacatgcgat aatcttcaat gcttttacca
     4561 aagcgcgccg cttcaatagg cgttaagcga tggtgcatgt catgcagcaa atgttcatta
     4621 aaaccaaact tgttacgtac cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc
     4681 gtcgaccaca gctgctcttc cggccactgt cctttggttg cgggttcata agccagacct
     4741 ttacctttcg acatgccata ggtggttttc ataccaggga ttccgcactg cacgcggatg
     4801 gctttgaatc ccagctcctg atgacgggca taatcatcca gagcttcatc aatactgtga
     4861 ccggtggtat ggcaataaac catcacccct tcacgagacg cgccgccgag taactggtaa
     4921 agcggcatgt tggcagcttt ggctttaata tcccacagcg ccatatcaac cgctgaaatg
     4981 gccgacatcg taaccggacc gcgacgccag taagcacctt tatagaaaaa ctgccagata
     5041 tcttcgatac ggtgtgcatc gcgaccaata agctgcggac aaaggtgatc ctgcaaataa
     5101 gaggccacgg aaagctcacg tccattgagg gtggcatccc caaggcccgt aataccgtcc
     5161 tcagtggtga tttttaatgt gacgaaatta cgccccggac aggtaacaaa aacttcagcc
     5221 tttacgatct tcatgttcga ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa
     5281 ctgagctact accatacaag tatgaagatc aaaaaaagcc ggagtgatca caaaaaaagg
     5341 cgtatatttg cgccgtgaat ggttgacaaa agatgaaata gaataccttt tgtcagctga
     5401 cacttcctcg tatcttattg ataaaaggga tttatgttcc tacgtgcgcc cccagcccgc
     5461 aacaatgatc aacatgccgc aaagcgcaat caacgccccc gtccagtcat aaagactcag
     5521 tttcacgcca tccacaacgc gcagccacat caacgccgtg cagacataaa cgccaccata
     5581 agcggcgtaa acacgcccac tcgccgcagg atgcaacgtt aacaaccaga caaacagcgc
     5641 cagtgaaatc cccgccggaa gcaacagcca gatactggcg tttcgtttta accacaacca
     5701 gggcagaaag catccaataa tttcacacag cgcagtagca aaaaatagta acgttgtttt
     5761 aatcatcttt gtcacttatt gacatcatgt atagttatag gacgacataa tatcatcaat
     5821 ataaacatcc tcctggtacg ttttgcgttc gcagtggatg gtgtagaatc acctttaatc
     5881 attcatacag ggaatgaatt atgaaaatta ctctcagcaa acgaattggc ctgctcgctt
     5941 ttctgctgcc ttgcgcactg gcattgagca caactgttca tgccgaaact aacaaactgg
     6001 tgattgagtc tggcgacagt gcacaaagcc gccagcacgc cgctatggaa aaagagcaat
     6061 ggaatgacac gcgcaatctg cgccagaaag tgaataaacg cactgaaaaa gagtgggata
     6121 aagccgacgc cgcttttgat aaccgcgata aatgtgagca aagcgccaac atcaatgcct
     6181 actgggagcc aaatactttg cgctgcctgg accgtcgaac tggccgcgtt attaccccct
     6241 aacctgttat tgatttaagg aatgtaagga cacgttatgc caagcgccca cagtgttaag
     6301 ctacgcccgc tggagcgtga agatttacgc tatgtacatc aactcgacaa taacgccagt
     6361 gtgatgcgtt actggtttga ggaaccctac gaagcctttg ttgaactctc tgatctgtac
     6421 gataagcata ttcacgatca gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa
     6481 gccggtctgg tggagctggt ggaaattaac catgttcatc gccgcgcaga atttcagata
     6541 attatctccc cggaatatca ggggaaaggt ctggcaacgc gtgccgcaaa attagcaatg
     6601 gactatggct tcaccgttct caatctctat aagctgtatc tgatcgtcga taaagagaat
     6661 gaaaaagcga ttcacattta ccgcaagctt ggctttacgg ttgaaggtga attgatgcat
     6721 gagttcttta ttaatggtca atatcgtaat gccattcgca tgtgcatatt ccagcatcag
     6781 tatctggcag agcacaaaac accgggtcag actctcctga agccgaccgc acaatagcat
     6841 taataataat cgatagtatt tttgatggtg taaacccgtt cgacggcggg ttttactcct
     6901 tcatcaacaa tgattagctg acagtccacc ggattggcgt gactgtcata ttcacagctc
     6961 tgttttacat taccaaccct ttgattattc agtaaagtaa ccgctgtgta atctaatttt
     7021 ttgatcggat ccgttgatgg cgtagcgctg acagataatg ttttgtcgtt acttttcgtg
     7081 gttttaccca gcggataccc ctgatcatca tagcgatatt ccatctgcat ctgtttgctg
     7141 ctggctttaa tcacaaagcc attatcatcg gtttcccagc tcaccccggc agaaggtaat
     7201 tctgctagct ggcattttcc ctgcaaacga actctcttct ccagtgtctg ggcatcacgg
     7261 taataattgg cgtccagtac cagggcaacc acggtattat tttccagatc cagtaattcg
     7321 agtgaatcaa aacagccttc ttccgacaaa gtccccgaaa cacgtttcgt cacttcacct
     7381 tgctcatcca ttaatgtctg agtgaaatct tttaccggac cacgcagcgg atcaaaatcg
     7441 aattcattag agaaactggc catctcaggg gtaaatgaaa gtgttacctc tgtgcggtca
     7501 catcctgtga ggaatatcgc gagtaagcat ggcagtaatt tgtatttcac aacagtcacc
     7561 agagagtaga gatgattctc aatcatagta gcaaatacag tactttacac gttaaatgct
     7621 atgcttaaag aagttatctt cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc
     7681 cgcgcttctg ctcgccctcg cctcacccgc ggtactcgcc gcgccgggat cctgtgagcg
     7741 catacagagc gatatatcac agcgcattat caataatggc gtacccgaaa gcagcttcac
     7801 gttaagtatt gtacccaatg accaggttga tcagcctgat tcccaggtcg tcgggcattg
     7861 cgctaatgat acgcataaaa ttctctacac ccgcaccacc agcggtaacg tctctgctcc
     7921 cgcgcagtct acccaggatg gcgcgcctgc cgaaccgcag taatacatta tcgactgcac
     7981 gccggatacg acacatccgg cgatttgaac atacaacata atcccacctt attactcata
     8041 cccttctatt gatatggatt aataattctt aacccaaaat aggtagactc cctctattgt
     8101 tagcgcgcta aatattcaat atataaactt ttatataacg ataaagaaca gggagtgagt
     8161 tatgtccaaa aatgaacaaa tggtgggcat cagccgcaga acactcgtta aatctaccgc
     8221 gataggttct ctggcgctgg ctgcaggcgg tttttcttta ccgttcaccc tgcgcagtgc
     8281 agcggcagcg gtacagcagg ccagcgagaa agtggtctgg ggtgcctgtt ccgtcaactg
     8341 tggtagccgc tgtgcacttc gtctgcatgt taaagataat gaagtgacct gggtggaaac
     8401 tgacaacacc ggcagcgatg agtatggcaa tcatcaagta cgcgcctgtt tgcgcggtcg
     8461 ctccatccgt cggcgtatta atcatcccga ccgcttgaat tacccaatga aacgcgtggg
     8521 caaacgcggc gaaggcaaat tcgaacggat tagctgggat gaagccctgg atactatcgc
     8581 cagtagcctg aaaaaaaccg tcgaacaata tggcaatgag gctgtatata ttcagtactc
     8641 ttcagggatc gttggcggca atatgacccg ctcttcgcca tccgcctcgg cggtcaaacg
     8701 cctgatgaac tgctacggcg gttcactcaa ccagtatggc tcctacagca ccgcgcaaat
     8761 ctcctgtgcc atgccctaca cctacggcag taatgatggc aacagcacca cggatattga
     8821 aaacagcaag ctggtcgtga tgtttggtaa caacccggct gaaacccgca tgagcggcgg
     8881 tggcatcact tatcttcttg aaaaagcgcg cgagaaatcg aacgcaaaaa tgattgttat
     8941 cgatccgcga tataccgata cggctgctgg tcgtgaagat gaatggcttc ctattcgccc
     9001 gggcaccgat gccgcgctgg ttgcgggtat tgcctgggta ttggttaacg aaaatctcgt
     9061 tgatcaacct tttctcgata aatactgcgt cggctatgac gaaaaaacct tacccgcaga
     9121 tgcacccaaa aatggtcact ataaagccta tattcttggt gaaggtgatg ataaaacagc
     9181 gaaaacgccg cagtgggctt cgcaaattac aggtatcccg gtagaccgta tcatcaaact
     9241 ggcgcgtgaa attggcacag caaaacccgc ttatatctgc cagggctggg ggccgcaacg
     9301 ccaggcaaac ggcgagctga cggcacgcgc tattgctatg ttgcctattt tgacgggcaa
     9361 tgtcggcatc agcggcggaa atagtggtgc gcgtgaatcg acctacacca ttaccatcga
     9421 acgcctgccg gtgctggata atccggtcaa aacgtcaatc tcctgcttca gttggacaga
     9481 tgctatcgat catggcccgc aaatgacggc aatccgcgac ggcgtccggg gcaaagataa
     9541 actggatgtg cccattaagt tcatctggaa ctacgcggga aataccctgg ttaatcagca
     9601 ttctgacatc aacaaaacgc atgaaattct gcaggacgaa tcgaaatgcg aaatgattgt
     9661 ggtcatcgaa aactttatga cctcatcggc aaaatatgcc gacattctgc tgccagacct
     9721 gatgaccgtt gagcaggaag atattattcc taacgactac gccggtaaca tgggatatct
     9781 cattttcctc cagcctgtca ccagcgaaaa attcgaacgc aaaccaattt actggatcct
     9841 gagtgaagtc gcgaaacgtc ttggacctga cgtctatcaa aagttcactg aaggtcgcac
     9901 gcaggaacaa tggttacaac atctgtacgc caaaatgctt gccaaagatc cggcgttacc
     9961 gtcttacgac gaattaaaaa aaatgggtat ctataagcgt aaagatccca atggtcattt
    10021 tgtcgcctac aaagcatttc gtgacgaccc cgaggcaaat ccacttaaaa cgccttccgg
    10081 taagattgaa atttattcca gcaagctggc ggaaattgcc cgtacctggg aactggaaaa
    10141 agatgaagtg ataagcccac tgccggttta tgcctcaacc tttgaaggct gggactcccc
    10201 tgaacgtagt acctttccac tgcaactgtt tggtttccat tacaaatccc gtactcactc
    10261 gacctacggc aacattgatg tcctgaaggc tgcctgccgt caggaggtgt ggatcaaccc
    10321 tatagacgca cagaaacgtg ggattgccaa cggcgatatg gtgcgggtgt ataaccatcg
    10381 tggcgaagtt cggctaccag ccaaagtgac accacgcatt ctccctgggg ttagcgctat
    10441 gggccaggga gcctggcacg aggccaatat gtctggcgat aaaatcgacc atggcggctg
    10501 tgtgaatacg ctaaccactc tgcgcccttc accactggcg aagggaaacc cgcagcacac
    10561 taatctggtc gagatcgaaa aaatataacc cactacaacc ataaattctg gcatgacatt
    10621 ttgtttgaaa agcaataagt gagtaatgat gaaaatccat accacagagg cgctcatgaa
    10681 ggctgaaatc agccgtagaa gtctgatgaa aacctccgca cttggcagtc tcgcgctggc
    10741 aagcagtgct ttcactctgc cattttccca aatggtccga gcggcagaga caccggtaga
    10801 agagaaagcg gtctggagtt cctgcaccgt taactgcggg agccgctgtc tgttacgttt
    10861 gcatgtgaaa gatgacaccg tgtactgggt ggagtctgat acgacaggag acgacgttta
    10921 cggtaatcat caggttcggg catgtttacg cgggcgctct atccgccgac ggatgaatca
    10981 tcctgatagg ttgaaatatc ccatgaagcg cgtcggcaag cgcggtgaag gtaaatttga
    11041 acggataagt tgggatgaag ccctggatac catcagtgat aatcttcggc ggatccttaa
    11101 agattacggc aatgaggctg tacatgtcct gtacggaaca ggcgtagatg gcggaaacat
    11161 caccaactcg aacgtcccgt accgtctgat gaactcttgc ggtggttttc tcagtcgcta
    11221 tggcagctac agtaccgctc aaatcagtgc cgcaatgagt tatatgttcg gtgccaatga
    11281 tggcaacagc ccggatgata tcgccaatac gaaactggtc gttatgttcg gaaataaccc
    11341 ggcagaaacc cggatgagcg gcggtggtgt cacttactac gtcgagcaag cccgagaacg
    11401 ttcaaacgca cgcatgatcg tcatcgatcc acgttataac gacacagctg ccgggcgtga
    11461 agatgaatgg ctgccaattc gcccaggcac cgatggcgca ctggcctgtg cgattgcctg
    11521 ggtactgatt actgaaaaca tggtcgatca gccatttctc gacaaatatt gtgttggtta
    11581 cgatgaaaaa acgctgcccg ccaacgcacc acgtaacgcg cattataaag cctatattct
    11641 gggcgaaggg cctgacggca tagctaaaac gccggaatgg gcagcaaaaa tcaccagcat
    11701 cccggcagaa aaaattatcc agttggcacg agagatcggt tcagcaaaac ctgcttatat
    11761 ttgtcagggt tgggggccac aacgacactc caacggcgaa caaacgtccc gcgctattgc
    11821 catgctttcc gttctcaccg gtaacgtcgg cataaacggc ggcaactcag gtgtacgcga
    11881 aggtagctgg gatctggggg tagaatggtt cccgatgctt gagaatcctg ttaaaacgca
    11941 gatttccgtc tttacatgga cagatgccat cgaccacggt acggaaatga ccgcgacccg
    12001 tgatggtgtt cgtggaaaag aaaaactgga tgtccccatc aagtttttat ggtgctacgc
    12061 cagtaacaca ttgatcaatc aacatggcga catcaatcac acccatgagg tgcttcagga
    12121 tgacagcaag tgcgagatga ttgttggcat tgaccacttc atgactgcct cggctaagta
    12181 ttgcgatatc ctgttgcccg acctgatgcc aacagagcaa gaagacctta tctctcacga
    12241 atctgccggg aatatgggct atgtgatcct cgcccaaccc gcaacctcag caaaatttga
    12301 acgcaaaccc atctactgga tgctgagcga agttgccaaa cgcttaggac cagacgttta
    12361 tcaaaccttt actgaaggcc gcagtcagca cgaatggatc aaatatctcc atgcgaaaac
    12421 gaaggaacgt aaccctgaga tgcccgacta cgaggagatg aaaacgaccg ggatctttaa
    12481 gaaaaaatgc ccggaagaac actacgtcgc tttccgcgca ttccgtgaag atccacaggc
    12541 aaacccgttg aaaacacctt cggggaaaat cgaaatttat tctgaacgac tggcgacgat
    12601 tgcggatacc tgggaattga aaaaagatga aattattcat ccccttcctg cgtatacccc
    12661 aggttttgat ggctgggacg atcccctgcg gaaaacctat ccactgcagc taacgggctt
    12721 ccactacaaa gcgcgtaccc actccagcta cggcaatatt gatgtgttac agcaggcctg
    12781 cccacaagag gtgtggatca accctattga tgctcaggca cgcggtatcc gtcatggcga
    12841 taccgtgcgg gtatttaaca ataatggaga aatgctgatt gccgcaaaag tgactccgcg
    12901 tattctgcct ggcgttaccg ccatcgggca aggtgcgtgg cttaaagcgg atatgtttgg
    12961 tgaccgggtc gatcacggcg gcagtatcaa tattctgacc tctcaccgcc cttcaccgct
    13021 ggcaaaggga aacccgtcgc acagcaatct tgtccagatc gaaaaggttt aaggagtagc
    13081 ccatgaccac acaatatgga ttttttatcg attccagccg ctgtactggc tgtaaaacct
    13141 gcgaactggc gtgcaaagat tttaaagatc ttggcccgga agtcagtttc cgccgtattt
    13201 acgaatatgc tggcggcgac tggcaggaag ataatggcgt ctggcaccaa aacgtgttcg
    13261 cttattatct ctccatctcc tgtaaccatt gcgatgaccc cgcatgtaca aaagtctgcc
    13321 ccagcggcgc aatgcataag cgtgaagatg gctttgtggt ggttgatgag gatgtttgta
    13381 tcggctgccg ctactgccac atggcttgtc cgtacggcgc gccacagtac aatgctgaaa
    13441 aagggcacat gacgaagtgc gatggttgtt attcgcgcgt cgccgagggg aaacaaccca
    13501 tatgtgtcga atcctgcccg ctgcgtgcac ttgagttcgg tccaattgaa gagctgcgcc
    13561 agaagcacgg cactctggct gccgtcgcgc cactaccgcg cgcgcatttc acaaaaccca
    13621 atatcgttat caaacctaac gccaacagcc gcccgaccgg cgataccact ggttatctgg
    13681 ctaatccgga ggaagtgtaa aatgggaaat ggatggcatg aatggccact ggttatcttt
    13741 accgtattgg gtcaatgcgt agtaggcgcg ctgattgtta gcggaattgg ctggttcgcc
    13801 gccaataatg acgcagaccg tcagcgtatt gtccgtggca tgtttttcct ctggctgttg
    13861 atgggcatcg ggtttattgc ctctgtcatg cacctgggat cgccactgcg agcgtttaac
    13921 tcgctgaatc gaatcggagc ttctggtctg agtaacgaga ttgctgccgg ttccatattt
    13981 tttgccgttg gcggattatg gtggctggtg gcagtcatcg gtaaaatgcc gcaagcgttg
    14041 gggaaactct ggttacttgt cagcatggcg ctcggcgtca ttttcgtctg gatgatgacc
    14101 tgcgtgtatc aaatcgacac agtgccaacc tggtataacg gttacactac gctggctttc
    14161 ttcctgacag tattgttgag cggtccaata cttgctgcca caattctgcg ggcagcccgc
    14221 gttacattta ataccacacc atttgccatc attagcgttc tggcgctgat cgcttgtgcc
    14281 ggggtgattg tgctgcaagg tttgtctctg gcgtctattc actcatccgt acaacaagcc
    14341 agcgcactgg taccagatta cgcctcctta caggtctggc gtgtggtatt gttatgcaca
    14401 ggtctaggat gctggctttg tccgctcatt cgtcgccgtg aacctcacgt tgccggactt
    14461 attctggggc taatcctcat acttggcggt gagatgatcg ggcgtgtgct cttttatggc
    14521 ctgcacatga cggtaggaat ggctattgca ggttaacaaa ttggtgcggg gcaacccgca
    14581 catttcagga tgttaggaat gacccatttt tcacagcaag ataatttttc tgtcgcggcg
    14641 cgcgtgttgg gcgcgctgtt ttattacgct ccagagagtg cggaagccgc acctctggtt
    14701 gcggtactca cccgtgacgg ctgggaaact cagtggcctc taccagaagc gtcattagcg
    14761 ccgctggtga cggcttttca gacacagtgt gaagagactc acgcccaggc ctggcagcgt
    14821 ttgtttgtcg gcccgtgggc actgccgtct ccgccgtggg gatcggtctg gctggatcgc
    14881 gaatctgtgc tgtttggcga ttcaacattg gcacttcgtc agtggatgcg cgagaaaggc
    14941 attcagtttg aaatgaagca aaacgaaccg gaagatcatt ttggttcact gctgttgatg
    15001 gctgcgtggt tggcggagaa tggtcgccag acagaatgcg aagaattgct ggcatggcac
    15061 ctttttccgt ggtcaacacg atttcttgac gtttttatcg aaaaagcaga acaccctttc
    15121 taccgtgcac tgggggaact ggctcgctta acgctggcgc aatggcaatc acaactgttg
    15181 attcctgtcg cggttaaacc actctttcga taggatcacc gtaatattgc cggatggtgg
    15241 cgattacgcg caattcggca ataataggtt acagtgtcac gtttttttat ctcttaaagc
    15301 acgcactgct tttgcggctg gcctcttttg ccgcaaaata gtcgcccgtg tttcattgcc
    15361 catttctgct catgcatcat ctacacatct atccggatct gcgcactatg ttccgccgtc
    15421 tgcttatcgc aacagtcgtc ggtattcttg cggcctttgc cgttgccggg tttcgtcatg
    15481 caatgctgct actggagtgg ttgttcctca ataatgactc gggcagtctg gtcaatgcag
    15541 cgacaaatct ttccccatgg cgacggctgc taactccggc gctcggcgga ctggcggcgg
    15601 gtttgttgct gatgggctgg cagaaattta cccagcaacg ccctcatgcg ccgaccgatt
    15661 acatggaagc gttgcaaacc gatgggcagt ttgattacgc agcaagcctg gttaaatcgc
    15721 ttgcctctct gctggtagta accagcggca gtgcaattgg tcgcgaaggt gcgatgattc
    15781 ttttagctgc ccttgccgcc tcctgttttg cccaacgttt tacgccacgc caggagtgga
    15841 aattatggat cgcctgtgga gctgcggcgg gaatggctgc ggcctatcgc gccccgcttg
    15901 ctggcagttt atttatagcc gaagtgctgt ttggcactat gatgttggcc tctctcggcc
    15961 cggtgattat ttccgccgtc gtggcgttgc tggttagcaa tctgattaat catagcgacg
    16021 cgttactcta cagcgtacaa ctctcagtga cggttcaggc tcgtgactat gcgctgatta
    16081 tcagtacagg tgtgctggca ggtctgtgcg gaccactgtt gttaacgtta atgaacgcct
    16141 gtcatcgtgg atttgtaagt ctcaaacttg cgccgccctg gcaactggca ctgggcgggt
    16201 tgatcgtggg tctgctttcc ctgttcacac ctgcagtgtg gggcaacggc tatagcaccg
    16261 tacaatcctt tttaaccgcc ccgccactgt taatgatcat tgccgggatc ttcctttgta
    16321 aactgtgtgc cgtgctggcg agtagcggtt ccggcgcacc cggtggtgtc tttacaccga
    16381 cgctatttat cggtcttgcc attggcatgt tgtatggtcg tagcctggga ttatggttcc
    16441 ctgatggcga agaaattacg cttttactcg gattgaccgg gatggcgaca ctgttggcgg
    16501 caaccacgca cgcgccgatt atgtcgacgt tgatgatatg tgaaatgacc ggggagtatc
    16561 agctactccc cggtttattg attgcctgcg taattgcgtc ggtaatttcg cggacgttac
    16621 accgtgactc tatctaccgc cagcacactg cgaagcatag ctaagcggat gtattgcccc
    16681 agttcacgct gttcggcgcg cggcagataa ggcagttcac caatgagcgg tgccggaagt
    16741 tttttaccga gcacatcaat gatttccgca taatgcgcca gtcctggatt gattcggtta
    16801 gccacccagc caatgagcgg cagcccgtcg ttggcaatcg cctgagctgt tagtagtgca
    16861 tggttaatgc aaccttcctg aataccgaca accatcaata ccggcagttg ttcctgcacc
    16921 acccattcag agagcggacg caaatcattc atcagactgc gccagccgcc agtcccttct
    16981 accacgacat gatcgacttt atcggtcagg tttgccaggc cgtttgaaat gagggtgtaa
    17041 ttgattgggc aactgtgcgc cacgctactt tcttcttcgc ttaacgcgat aggattaact
    17101 gcttcataag gcagttcgat ggttgaaaca ctctgcaaca ccagggcatc tttattacgc
    17161 agcccttcgg gtgtctcttt gctccccttc gctacgggtt tatatcccgc aaccgttttt
    17221 ccctgggagg ctaacgcttg tagcaatgcg cgggaaacca ccgttttccc tacagaagtg
    17281 tctgtaccgg taataaagaa acgcttcagc atcactaact ccaccgttat gcttcacaaa
    17341 tataaaccag gaaaataatt aaccttgaaa gtctaagtta tgctttcctg gcccaaattg
    17401 agatagcgca aattttggta gaacagttaa aaaatgttaa ccctgcaaca gacgaatcaa
    17461 caaagaaccg ttatacatcg cgtcttttac cagcgcagcc cctgccatcg taccctggtt
    17521 agaaaactga gtactctcaa cgctaatgtg ctgactatac gcaggaaggg cctgctgacg
    17581 gatgctgtcc gagatgaccg ggaagaggat atctgccgct ttacttaacg gtgagccaat
    17641 cagtattttt tgtgggttaa ataaattcac catgatggca agaatgcgcc ctacatgcgc
    17701 gcccaccccg gtaatgatgt cttttgccag tagatcgccg cgcaatgccg cctgacacaa
    17761 tgaatccacg gttaacggct gtccatgtaa catcgagctc atggattgat tgagacgcag
    17821 ctgtgccagc tcaagaatac tgtccacact ggcgatggtt tcgaggcagc cgtgattccc
    17881 gcaataacag cgtttcccat acgggtcgac ctgcgtgtga cctatttcca cgaggctact
    17941 gctgcctgcg tgtagcagat gaccatcggt aatgacgccc gcccccacgt tgtgatcgat
    18001 aaccacctga atcacatcgc gcgccccgcg tgaggcacca aacaaggcct cagccatcgt
    18061 ccatgcgctg atatcatgct gaatataaac cggtacgccg gtatgctgct ccagcgcctc
    18121 gccgagcggc atctctttta catcctcgta gaacggcatg cgatgtacaa taccattttc
    18181 cgtatcaata attcccggca aggttatggc aatcgaagtc agacgctcaa gttttttctg
    18241 gtggcggata aaaaactgat cgatatggga aatgatacga tccagcaatg gcgagtcatc
    18301 ttttaacgcc agttcctgcg actcctccac taccagtttg ctgctcagat cgcgcaaagc
    18361 aaggaaaatc tccccgcgac taatgcgcag agaaagatag tgccaggctt cagtttcaac
    18421 caccagcccc actgccggac ggccacggtt ccccgcttct ttgatttcca gctcttgcac
    18481 caggtgtgct tcgagcatct cacggacaat tttagtgata ctggcaggag ccagctgcgc
    18541 cagacgggaa agatcgatac gcgagactgg accaagctga tcaatcaggc gataaaccgc
    18601 gcccgcgttg gtctgcttta tttgatcaat gtgcccaggc tgattttcag caaccaccgc
    18661 atactcccta tattttcgcg ctccgaaata atctgtaggc tatggtgaag cacttcaata
    18721 cgtgtcgtca aatttttact taggcatgtg attaacagca catttttcgg gctttttcgc
    18781 tgaaatttcc ctcacctgag agcattcagc agatgaatac gaaagttacg cgcagccgga
    18841 ctttgttcat ggtgtttcgg ccagaccaac cacatttcag aaaccgcatc ctcttcagca
    18901 atcggcaccc agcgcatttc gttgagctga acacgtttaa atgacgcagg caaaattgaa
    18961 acacccagac cagcggaaac cagaccgatg atggtcattg cctcacccac ctcctgagtg
    19021 atgacgggcg tcaaattgta acgtcgcatc agcccgagaa tatcgtcata cagccctgtc
    19081 cctacgtgcg gatcaaaaaa gacaaagggt tctttcgcca gttcagccag cgttacactc
    19141 gggttatttg ccaagggatg atcgtgcggg atcatcgcca taagcggttc atggacgatg
    19201 actgcgtgtt caagcgtctc cggtaacgct gtgttacgca gcaatcccat atccagcgtt
    19261 ccttcaatga gcggagcgat ttgctcgcgg gtgttcattt cgcgggtttg taaatggaca
    19321 tcgggataat cacggcgaaa cagcgataac gtatcggaca ccgcccgaat aaaaggagcg
    19381 gacgaagtaa aaccaatgcg caactccccc gcttcaccct gatgcagtct ttcagcgcga
    19441 gcggcagcgt catccatcat agacaggatt tgccgactat ctgcaagaaa ctgttttcct
    19501 gctgccgtca gcaatacact gcgattagtt cgtgccagca gtcgggcacc aatttgttgc
    19561 tccagcgcct gaatctgctg acttagcggc ggttgcgaaa tattcaggcg ggcagcggcg
    19621 cgcccgaaat gcagctcttc tgcaacagca acaaagtaac gcagatgacg aagttcaata
    19681 ttcatattta aaacatctta tttgagatta ttaatatatt agacagaaca attcgatttt
    19741 cctaatctat tcataagcct gaaccacagg catatttagc aaggatttca agtgagccgt
    19801 actacaactg ttgatggcgc tccggcaagc gacactgaca agcaaagcat ttctcagcca
    19861 aatcaattta ttaaacgcgg tacgccgcaa tttatgcgcg tcaccctggc gctgttctct
    19921 gccggactgg caaccttcgc acttctctat tgtgtgcaac ctatccttcc ggtgctttcg
    19981 caggagtttg gcttaacgcc cgcgaacagt agtatttcac tgtccatttc tacggcgatg
    20041 ttggctatcg gtttgctgtt tactggcccg ttatccgatg ccattggtcg caaaccagtg
    20101 atggtcacgg cgctactgtt ggcctccatt tgtacgttac tttcgacaat gatgaccagc
    20161 tggcacggca ttttgattat gcgcgccttg attgggcttt cgttaagtgg cgtagcagct
    20221 gttggcatga cttatcttag cgaggaaatc caccccagtt tcgtggcctt ttcgatgggg
    20281 ttgtatatca gcggcaactc aattggcggc atgagcggac gcttaattag cggtgtcttc
    20341 acggactttt tcaactggcg aattgcgctg gcggcaatcg gttgtttcgc gctggcctcg
    20401 gcgttaatgt tctggaaaat cctccctgaa tcacgccatt ttcgcccgac ttcactgcgc
    20461 cctaagacgt tattcatcaa ctttcgtctg cactggcgtg accggggatt accgttattg
    20521 ttcgcagaag gctttttgct gatggggtcg ttcgtcacgc tgtttaatta cgtcggctat
    20581 cggttgatgc tctccccctg gcatgtcagc caggccgtgg ttggcttatt atcgctggct
    20641 tatttgaccg gtacatggag ctcacccaaa gccggaacca tgaccacccg ctatgggcgc
    20701 ggtccggtga tgttgttttc gacgggggtt atgctgtttg gtttattgat gaccttattc
    20761 agctcgctgt ggctgatctt tgccggaatg ttactcttct cagccggatt cttcgcagcc
    20821 cactcagtag ccagcagctg gatcggcccc cgcgcaaaac gcgctaaagg ccaggcctcc
    20881 tcgctgtatc tgttcagtta ctatctgggg tcgagtattg ccgggacgct gggtggtgtt
    20941 ttctggcata actatggctg gaacggcgtc ggcgcattta ttgctctgat gctggtcatt
    21001 gctctgctgg tcgggacgcg tttgcatcat cgtctgcacg cctg
//
LOCUS       DAZGHF010000082        20536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGHF010000082 DAZGHF010000000
VERSION     DAZGHF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.082"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1261)
                     /locus_tag="ACHK3A_003148"
     CDS             complement(<1..1261)
                     /locus_tag="ACHK3A_003148"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO8741199.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNG"
     gene            complement(1273..1560)
                     /gene="eutN"
                     /locus_tag="ACHK3A_003149"
     CDS             complement(1273..1560)
                     /gene="eutN"
                     /locus_tag="ACHK3A_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO8741200.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(1667..1960)
                     /gene="eutM"
                     /locus_tag="ACHK3A_003150"
     CDS             complement(1667..1960)
                     /gene="eutM"
                     /locus_tag="ACHK3A_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO8741201.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(1999..3015)
                     /gene="pta"
                     /locus_tag="ACHK3A_003151"
     CDS             complement(1999..3015)
                     /gene="pta"
                     /locus_tag="ACHK3A_003151"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO8741202.1"
                     /translation="MIIERCRKLALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFALRWLARAGEKTPPDALDKLTD
                     PLMFAAAMVSAGTADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVQRQTEVNRESSLQ
                     TLVE"
     gene            complement(3012..3815)
                     /gene="eutT"
                     /locus_tag="ACHK3A_003152"
     CDS             complement(3012..3815)
                     /gene="eutT"
                     /locus_tag="ACHK3A_003152"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO8741203.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQSLTVKQIQQRLGETQ"
     gene            complement(3812..4513)
                     /gene="eutQ"
                     /locus_tag="ACHK3A_003153"
     CDS             complement(3812..4513)
                     /gene="eutQ"
                     /locus_tag="ACHK3A_003153"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO8741204.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(4488..4967)
                     /gene="eutP"
                     /locus_tag="ACHK3A_003154"
     CDS             complement(4488..4967)
                     /gene="eutP"
                     /locus_tag="ACHK3A_003154"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO8741205.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(4980..5315)
                     /gene="eutS"
                     /locus_tag="ACHK3A_003155"
     CDS             complement(4980..5315)
                     /gene="eutS"
                     /locus_tag="ACHK3A_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO8741206.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(5608..7887)
                     /gene="maeB"
                     /locus_tag="ACHK3A_003156"
     CDS             complement(5608..7887)
                     /gene="maeB"
                     /locus_tag="ACHK3A_003156"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO8741207.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYRGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            8176..9126
                     /gene="tal"
                     /locus_tag="ACHK3A_003157"
     CDS             8176..9126
                     /gene="tal"
                     /locus_tag="ACHK3A_003157"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO8741208.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            9146..11149
                     /gene="tkt"
                     /locus_tag="ACHK3A_003158"
     CDS             9146..11149
                     /gene="tkt"
                     /locus_tag="ACHK3A_003158"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO8741209.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKYAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(11252..12295)
                     /gene="ypfG"
                     /locus_tag="ACHK3A_003159"
     CDS             complement(11252..12295)
                     /gene="ypfG"
                     /locus_tag="ACHK3A_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO8741210.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(12421..12996)
                     /gene="nudK"
                     /locus_tag="ACHK3A_003160"
     CDS             complement(12421..12996)
                     /gene="nudK"
                     /locus_tag="ACHK3A_003160"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO8741211.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     NKALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(13064..15043)
                     /gene="aegA"
                     /locus_tag="ACHK3A_003161"
     CDS             complement(13064..15043)
                     /gene="aegA"
                     /locus_tag="ACHK3A_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO8741212.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QVQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            15249..16949
                     /gene="narQ"
                     /locus_tag="ACHK3A_003162"
     CDS             15249..16949
                     /gene="narQ"
                     /locus_tag="ACHK3A_003162"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO8741213.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            17113..20226
                     /gene="acrD"
                     /locus_tag="ACHK3A_003163"
     CDS             17113..20226
                     /gene="acrD"
                     /locus_tag="ACHK3A_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO8741214.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAQLVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(20325..20384)
                     /gene="ypfM"
                     /locus_tag="ACHK3A_003164"
     CDS             complement(20325..20384)
                     /gene="ypfM"
                     /locus_tag="ACHK3A_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO8741215.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         4703 a         5593 c         5341 g         4899 t
ORIGIN      
        1 gtccgttctt aacgaaaatg ctggtatcaa tggcattcgc catctggttc atgttctcga
       61 tattgcgcga gtgcattgcc gccgtgtggt ggcaaccgcc ttccagtttc accgccagcg
      121 caatggcatc cgccacgttg gcgacgcgca cgacgggcag caccggcatc atcagttcag
      181 tcacagcaaa cggatgttct gcggtggttt ctacaaacag cagtcgcgtt tgttccggaa
      241 ctttaaggcc gattgccgcc gcgattttcg ccgcatcgcg accaacccag tcacggctga
      301 cggtgccttt tccgcgctcg tcgatatttt tcagcaacac cgattgcagt tgctgcgcct
      361 gctctgcggt cagtttcacc gcatgctggc cttccatcag acgcatcagt tcatcggcta
      421 cgctatcaac aacaatcagt accttttcgt cggcacaaat gatgttgtta tcgaaagaag
      481 cacctttgac gatggactga gctgcacggg cgaggtcggc ggtttcatcc accactaccg
      541 gcgggttacc ggcacctgcc gcaatcaggc gtttattggt gtgtttgcgc gccgcttcca
      601 ctaccgcttc gccgccggtc accaccagca ggccgatacc cggaaacttg aacaagcgtt
      661 gcgcggtttc gatatccgga tttgctacgg taaccagtaa gttttccggc ccgcctgcgg
      721 caacaatcgc ctggttgagc agcgtaatcg cccgctggga gacttttttc gccgccggat
      781 gcggggcaaa aatgacgctg ttgcccgcgg caatcaggct gatggcgtta ttaatgacgg
      841 ttgccgccgg gttagtggaa ggcgtcaccg aagccactac gccccagggc gcgttttcaa
      901 ttaaagtcag gccgttgtcg ccagtcagca cctgtggaga gaggcactca acgcctggtg
      961 tgccgcgcgc ctgagcgacg tttttggcaa atttatcttc aacgcgcccc atgccggttt
     1021 cactgacggc aagttccgct aaatctctgg cgtgtttttc gcctgcttca cgaatggcag
     1081 caatggctaa ctggcgcatt gccacgcttt ttaacccttg ctgggcgact ttggctgccg
     1141 caacggcgtc atccagggac gcgaaaacgc ccatctcatg aacggcggcg ggcggcgtgt
     1201 cactgctttg cattttcagc agtaccgctt tcaccacctg ttcaatatcc tgttgattca
     1261 tgatgttctg ccttatttgt ggaaaatgac ctgaccgcca gacaccacct catcgacgat
     1321 gccaatcacg cacagatcga ccggtgacgt ttcgctttta tgcgcctggc gggcggaact
     1381 gccactcacc agcaacaccc actccccggt tcccgcgcca atattgtcga tggcgacggc
     1441 gcattgcccg tcaggattac cttgtggatc aatcatttcc accatcagca atttgtcatg
     1501 cgccagtccg tgatggcgta cggtacaaac aatttgtcca gtgacgactg ccagtttcat
     1561 acccgcctcc gtggcgtatt tcaggtaaaa gctcccccta ccctccgcag aaggtaaaat
     1621 gaaaaaggag agagcgtgac gcccgaatcg acgtcacaca gggtgattac aggttgctgc
     1681 tatcgccttt caggccgatc gggaagactt cttccaggtc gccgtgcgga cgcgggataa
     1741 cgtgtacaga taccagctcg ccgatacgct gtgccgctgc ggcacctgca tcggtcgcgg
     1801 ctttacaggc tgcaacatca ccacgaacca tggcagtaca caggccgccg ccaatctgct
     1861 taacaccaac cagcttgacg cgcgcagctt taaccatcgc gtcagaagcc tcaatcagtg
     1921 caaccaggcc ccgggtttcg atcattccta atgcttccat tgtgctttcc tctttatcag
     1981 ggtccagaac gggaccgttc attcaaccag tgtttgtaaa ctgctttcgc ggttcacttc
     2041 tgtctgacgc tgcacagctg ccaccagcgc cagttcgata atttcctgca cgctacaacc
     2101 acgagagaga tcgtgcatcg gcgcggcaag tccttgtatc aatggcccga cggcacgata
     2161 tccgccaagt cgttgtgcga ttttgtagcc aatatttccg gcttccagcg acggaaaaac
     2221 catcacatta gctttgccct gtaacgggct ggcaggcgct ttttgcgccg ccacttccgg
     2281 cacgaaggcg gcgtcaaact gcaactcgcc atcgacaacc agctttggtg cgcgctcacg
     2341 gacgatttct gtcgcctgct ggacgttggc aacgcagggg tgacgggcac taccgttgct
     2401 ggaaaacgac aacatcgcca cgcgcggttc ttctccggtg atggcgcgcc aggtttcggc
     2461 gctggcaagc gcgatatccg ccagttgcgc cgccgtcggc tgtggcacta cgctgcaatc
     2521 ggcaaagccc aatgcggggc cactgtactg tggcagcatc aggaaaatgg aggagagcgt
     2581 tttacagcct ggctgtaagc cgataatgcg taatccggca cgcagcacat tcgccgtgga
     2641 agagagattg cccgcgatac agacatccgc tgtcccggcg ctgaccattg cggcagcgaa
     2701 catcagcgga tcggtgagtt tatccagcgc atccggcggc gttttttcgc ccgcacgggc
     2761 cagccagcgg agagcaaatt cttcccgcat tgcgaggttg ccatgcggat caatcacctg
     2821 tagcccgtcc atcgccacgc cgtgactgag cgcaaactga cgaagttcaa acggattggc
     2881 gaccagaatg ggcgttgcca gaccttgttg atgtaaatat tgcgccgctt tcagcacacg
     2941 ttgatctaac gcatccggaa aaactactct ggcgggcgct cgcaacgcca gtttacgaca
     3001 acgttcaata atcattgcgt ttcccccagt cgttgttgga tctgcttcac cgttagcgac
     3061 tgcttcgtta cgctcaggat catcatcacg taaaccgtgc tggaaaggcg gttcagcgcc
     3121 tgcaagatgt ccgggcgtaa tacttcaaag ctgcgggtga taaacacctg cgccgctaca
     3181 gtttcggttt cccgcacttt ggtgcgtagc agattgagca acgccgcatc acgcccgtgg
     3241 ctggcttccg gcaccagatg atcgtggtcg agatagcgca gcggctggtg agaaagccga
     3301 tgcagatctt cgtcagaaag cccgacaatc gcttgttcgc ccagcggttc ccccagcgca
     3361 tcggcgcgca taatgttgcc aagacgcgaa cggatatccg ccagccacgg ctgccacggt
     3421 tccgccagtt caatttgcag ccacactgcc agcgcaatgg tgctgtcgag aacagcgcga
     3481 aaagccaggc gcggatcgct tttggcaacc attttttccg ccgacaggtg agtcagcgta
     3541 tccggctttt ttgccaccgg ctggcggcac agttcgcagc acgcctgcgg atgttcatcg
     3601 ctactggtta gcccgtgaac gggctgcggc tgctgctgtt catcgtcaac aaacaggcgg
     3661 ccctgctcgt caataaactt gatgcgcaga tggcggcttt ccagtaactc ccgggcagag
     3721 ggcgtcaggc gactgtccgc agggagatgg atctctgcgc cttcgctgag cgtatgattc
     3781 gctcttagcc atgcttcggt gatgaaatct ttcataagga ttgccagtta gccggccagg
     3841 cgacatacag gaatttcacg ctggatgtcg taccaaattc gatgctggag cctttcggga
     3901 taaacatcac atcgcccgct ttggcgatca tagtttcgcc ttcgtggcga acatgcagct
     3961 ccccttccag caccatgtcg atttcgtcgt agttcagggt ccacggaaag aaggcgttct
     4021 cccactgcat gaaaccagcg gccatactgc tgccgtcgtc tccggtcacg agatcagtca
     4081 agcctacgca gtgcggttcc gcgccatcga aacgaccaaa cttgacgctg ctgccatcaa
     4141 taactttgat gccgcctttg ccggtcaccg atttaaagct cggctgcagt gcaccctgct
     4201 ccagcgactg tttttccttc atcacttttt ccatcagctg cgcgaccagg ctttcggtaa
     4261 actgaccttc cggcagttgg gcgataatgg tttcacggat gcgctggctt tcggttttat
     4321 cggctgatgc gctggcggga acagacgccg ttaccgggat cgattcatca cattcggtga
     4381 tggtaaaacc caacagatca gccacttcac gagcttccgg ggtaataatg ctggcgcgca
     4441 gaaccacgga cattgccagt tcgccgcgcg cgtgtgcttc acgaatatca ttcgctgtga
     4501 tgagtttttt cacctgcttc ctcctgtttg gtgagagatg ccagataatc caccagttgc
     4561 tgcacgcttt gcgggtcgtg gctgtttagc tcgaaaatcg gctcttcgaa gcccgtctca
     4621 agcaataact ttcgcgttgc ggcgacgtca gcatctggca tgtccgtttt actgatgacg
     4681 gcgatttgcc gtttgctgac gccaatatcc agcaacccgg caggcaaccg actttccggg
     4741 tcattcgcgc cgtggacata gatcagcatg tccacatcct gtagcgtggt aattaatgcg
     4801 tgataccagc ggggatggct aaaatattca cccggcgtat caatatcgcc attatcatta
     4861 aattccacgg cctgcgtttt tctggcgagg gtataattcc cctgcaacgc attaaataac
     4921 gttgtttttc ctgccccgac ggtacccaca aacgcaattc gtttcatgaa tgcctcatat
     4981 taacttttgg tcatttcaca aagcgtataa tttaataagc gtcctaaacc actgacggtt
     5041 tgtgataacg cttcttctac tgcgcccacc gaaccataaa tcaccagcgc gccgctaaaa
     5101 cggtcgagaa aaccgatatg cacatcggcg gctttcaacg ctaaatcacc ggcgatcatc
     5161 gccgtttctc cgggagttag cgtcataatg ccaattgcgc ccgcatccgg aacgccgatt
     5221 ttcttcgcca gttcctcgcc gggatgcgcg atgagatgcg ccagcgtgac ctgtttgccg
     5281 ggcacaaatt cctgaatgat gcgttcttta tccatgagtc gccgcctgtg aaaaactttc
     5341 gtgtatcgtg acaatttttc gtcagagtaa ataacaaaat tcggcaagcg cgtttaaaag
     5401 gtgaggtaga tcactaagaa tggagagata tttactgtta tgaataaact ttcatatgcg
     5461 acgcatgaat ataaataaaa taatatcgag atattttata tactgatttt tttattattc
     5521 gtaaactcag taataagctt tactaatata ttgcagctaa actgcttacc ctgaatattc
     5581 agggtaagcg tgagagttaa aaaaaaatta cagcggttgg gtttgcgctt caaccacggc
     5641 cagcgccacc atattgacga tacgacgtac cgatgcgatc ggcgttaaca cgtgaaccgg
     5701 tttcgccaca cccatcagca ccggaccgac agtcacgcct tccgagctgg aaacgcgcag
     5761 taagttgtaa ctaatgcggg cagcttccat gttcggcatc accagaatat tagcggaacc
     5821 tttcaaaggg ctgtccggca tacggtcatt ccgaatcgct tccaccagcg ctgcatcgcc
     5881 gtgcatttca ccatcaatca tcagttctgg tgcacgttcc ctgaccagtt ccagcgcctg
     5941 acgcattttg ctcgacgacg ggcagtcaga agaaccaaag ttggagtgcg acaacagagc
     6001 cacacgcggc tcaataccaa aacgacgaac agtttctgcc gccatcaagg tgatctccgc
     6061 cagctcttct gcatcaggct catcattgac gtaggtatcg gcaataaagg tgttaccact
     6121 cggcaacagc agcgcgttca ttgcacctgc ggtgtgaacg ccatcgcgat aaccaaagac
     6181 atttttcacc acgctaaagt gttcatgata atcgcccacc gtaccgcaaa tcattgcatc
     6241 ggcttcgcca cgctgaacca tgatcgcgcc gatcactgtc gggttactga tcagcgcacg
     6301 ctgcgcctgc tcctgagtga caccgcgacg cttcatgatc tggaagtatt cggtccagta
     6361 ctctttaaag cgcggatcgg attcgttatt gacgatctca aaatcaacgc ccgccttgat
     6421 ctgcaagccc agtttctgaa tgcgcatttc gatcacgttc ggacgaccaa taaggatcgg
     6481 tttcgccagt cccagcgtta ccagttcctg agtggcatgc agaacgcgcg cctcttcccc
     6541 ttccggcaga actacgcgct tcggcgcttt gcgagcctgg gagaaaatcg gcttcataaa
     6601 caggttagtt ttgtaaacga actccgtcag cttgtcgatg tagacgtcga aatcagcaat
     6661 cggacgcgtc gccacgcccg actccatcgc cgctttcgcc accgcaggag cgatcttaac
     6721 gatcaagcgc ggatcaaacg gttttggaat gatgtattcc ggaccaaagc tcagatcctg
     6781 atcgccatac gctgaagcca ccacttcact ctgttccgca tgggccagtt ctgcaatcgc
     6841 gcgtaccgcc gccagtttca tctcttcgtt gatggcggtt gcgccaacgt ccagcgcgcc
     6901 acggaagata aacgggaagc aaaggacgtt gttcacctgg ttcgggtagt cggaacggcc
     6961 ggtacagatg atggcatccg gacgcacttc tttcgccagc ggcggcagaa tttccggttc
     7021 cgggttcgcc agcgccagga tcattggcgc acgggccatt ttcttcacca tttcctgggt
     7081 cagcactttc gggccggaac agccgaggaa aatatccgcg ccttcaatca catcatcgag
     7141 ggtacgttta ccgtcatcca ccaccgcgta tgcggctttg gtttccgcca tgtttggctc
     7201 acggccccga tagataacgc cttttgaatc gcaaaccacg atgttatgtt tttgcagacc
     7261 cagcgctacc agcaggttca tacaggcgat tgctgcggca cccgcgccag aaaccaccat
     7321 ccgcacgtcg gagatgtttt tctccaccac gcgcaaaccg ttgaggatgg cggcagtgct
     7381 gataattgcc gtgccgtgct gatcgtcgtg gaataccgga atattcatcc gctcgcgcag
     7441 tttctgttca atatagaaac attctggcgc tttaatgtct tcgaggttga tgccgccaaa
     7501 ggtcggttcg agcgcagcga cgacttcaat aaatttgtcc gggtcgagtt catcaacttc
     7561 tatgtcaaat acatcaatcc cggcgaattt cttaaacaga acgcccttgc cttccatcac
     7621 cggtttgcct gccagcgcgc caatgttgcc taaccccagc accgccgtac cgttagagat
     7681 caccgctacc aggttaccgc gcgcggtata tttgtaggct tttaacgggt ctttttcgat
     7741 ttcaagacaa ggtgcggcaa cgcctggtga gtaggccagc gccagatcgc gctgcgttgc
     7801 cagaggcttg gttggagaaa cctggatttt ccctggaact ggaaattcat ggaaatcaag
     7861 tgcactttgt tttaactggt catccatttg gttgttcctt tcacgtaacg ttcacaaata
     7921 aagtgtgtgg gcaacagccc ctgcccacaa cgtggcgcac attattacct tgccggagtc
     7981 tacagacttt gagcaagtcc aaactctcac cattaatata atgttttggt aataatccta
     8041 taacactgat gttacctgct taatccagca ataccatgcc tgtctgctat gcttttttga
     8101 tgcgtttagc gaaatttctc agaagtgtga attaacgcac tcatctaaca ctttactttt
     8161 caaggagtat ttcctatgaa cgagttagac ggcatcaaac agttcaccac tgtcgtggca
     8221 gacagcggcg atattgagtc cattcgccat tatcatcccc aggatgccac caccaatcct
     8281 tcgctgttac tcaaggctgc cggattatca caatatgagc atttaataga cgatgctatc
     8341 gcctggggta aaaaaaatgg caagacccag gagcaacagg tggtcgcagc gtgtgacaaa
     8401 ctggcggtca atttcggtgc tgaaattctc aaaatcgtac ccggtcgcgt gtcaacagaa
     8461 gttgatgcac gcctctcttt tgataaagaa aagagtattg agaaggcgcg ccatctggtg
     8521 gacttgtatc agcaacaagg cgttgagaaa tcacgcattc tgatcaagct ggcttcgacc
     8581 tgggaaggaa ttcgcgcggc agaagagctg gaaaaagaag gtattaactg caacctgacg
     8641 ctgctgtttt cttttgcaca ggcgcgggcc tgtgcagaag caggcgtttt tctgatttcg
     8701 ccgtttgtcg ggcgtattta tgactggtat caggcacgca agccgatgga cccgtatgtg
     8761 gtggaagaag atccaggcgt taaatcagta cgcaatatct acgactacta taagcaacac
     8821 cactatgaaa ccattgtgat gggcgcgagc ttccgtcgca ccgaacaaat cctcgcctta
     8881 accggctgcg accgactgac tatcgcaccg aatttgctga aggagctaca ggaaaaagtt
     8941 tcgccagtgg tacgtaaatt aatcccacct tctcagacgt tcccacgccc agctcccatg
     9001 agcgaagcgg aattccgttg ggagcacaat caggatgcga tggcggtaga aaaactgtct
     9061 gaaggcattc gtctgttcgc cgttgatcaa cgcaaactgg aagatcttct tgccgccaaa
     9121 ctataaacca gccacggagt gttatatgtc ccgaaaagac cttgccaatg cgattcgcgc
     9181 actcagtatg gatgcggtac aaaaagccaa ttctggtcat cccggcgcgc cgatgggcat
     9241 ggctgatatt gccgaagtgc tgtggaacga ttttcttaaa cataatccta ccgacccaac
     9301 ctggtatgat cgcgaccgct ttattctttc caacggtcac gcgtcgatgc tgctctacag
     9361 tttgctgcat ctgaccggtt acgacctgcc actggaagaa ctgaagaact tccgtcagtt
     9421 gcattcgaaa acccctggtc acccggaaat cggctatacg ccaggtgttg aaaccaccac
     9481 cggtcctctt ggacaaggtt tggcgaacgc cgtcgggctg gcgatagcag agcgtacgct
     9541 ggcggcgcag tttaatcagc cggatcatga aattgtcgat cacttcacct atgtgtttat
     9601 gggcgacggc tgcctgatgg aaggtatctc tcacgaagtc tgttcgctgg cgggcacgct
     9661 ggggctgggc aagctgattg gtttttacga tcacaacggt atttccatcg acggtgaaac
     9721 cgaaggctgg tttaccgacg atacggcaaa acgttttgaa gcctatcact ggcatgtgat
     9781 ccacgagatc gacggtcacg atccgcaggc ggtgaaggaa gcgatccttg aagcgcaaag
     9841 cgtgaaagat aagccgtcgc tgattatctg ccgtacggtg attggctttg gatcgccgaa
     9901 taaagcaggt aaagaagagg cgcacggcgc accgctgggt gaagaagaag tggcattggc
     9961 acggcaaaaa ctgggctggc accatccgcc atttgagatc cctaaagaga tttatcacgc
    10021 ctgggatgcc cgcgaaaaag gcgaaaaagc gcagcagagc tggaatgaga agtatgcagc
    10081 ctacaaaaag gcccatccgc aactggcaga agagtttacc cgtcggatga gcggtggttt
    10141 accgaaggac tgggagaaaa cgactcagaa atatatcaat gaattacagg caaatccggc
    10201 gaaaatcgct acccgtaagg cttcgcaaaa tacgcttaac gcttacgggc caatgcttcc
    10261 tgaattgctc ggcggttcgg cggatctggc tcccagcaac ctcaccatct ggaaaggttc
    10321 tgtgtcgctg aaggaggatc cggcgggcaa ctacattcac tacggggtgc gggaatttgg
    10381 catgaccgct atcgccaatg gcattgcgca ccacggcggc ttcgtgccgt ataccgcgac
    10441 gttcctgatg tttgttgaat acgctcgtaa cgccgcgcgg atggcggcac tgatgaaagc
    10501 gcggcagatt atggtttata cccacgactc aattggctta ggggaagatg gtccgaccca
    10561 ccaggccgtt gagcaactgg ccagtctgcg cttaacgcca aatttcagca cctggcgacc
    10621 gtgcgatcag gtggaagcgg cggttggctg gaagctggcg gttgagcgcc acaacggacc
    10681 gacggcactg atcctctcca ggcagaatct ggcccaggtg gaacgtacgc cggatcaggt
    10741 taaagagatt gctcgtggcg gttatgtact gaaagacagc ggcggtaagc cagatattat
    10801 tttgattgcc accggttcag agatggaaat caccctgcaa gcggcagaga aattagcagg
    10861 agaaggtcgc aatgtacgcg tagtttcgct gccctcgacc gatattttcg acgcccagga
    10921 tgaggaatat cgggagtcgg tgttgccttc taacgttgcg gctcgcgtgg cggtggaagc
    10981 aggcatcgcc gattactggt acaaatatgt tggtctgaaa ggggcaattg tcgggatgac
    11041 cggctatggg gaatctgctc cggcagataa gctgttcccg ttctttggct ttaccgccga
    11101 gaatattgtg gcaaaagcgc ataaggtgct gggagtgaaa ggtgcctgat ggtgatcgcc
    11161 ggatgctata tgttcccctc accctaaccc tctccccaga ggggcgaggg gacggtccgt
    11221 gcgcttttta ggggcgcatc tgggattttg tttaacgggt gatccacaac gtgggccagg
    11281 cgtctggccc atgccagtta tcgcaggtgg gttctgcggc gtaacgcacc aggcgaaaac
    11341 gctgaccgtc aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg
    11401 ctaaggtcac cagttcacgc gatttttcat cgaatgttgc gttcatcagt tccagttcat
    11461 tcgtctcctg accgctgttg aacggcaaac gtaaccgaac cgggcgcgag gccagtggct
    11521 ttttacgcga cacaatccat gccaaatcaa tggtgttata ggcccctgcc tcacagctaa
    11581 tcatcatcag cgctttatca tcggtcagcg cagtgacatt cacctctcga cgcaatggat
    11641 caagcgagca gcgcagacca ttcatccgcc agtttccata atccagcaaa tcgttgcgtt
    11701 cttcgagtga gagtggcgtc ggcgttgggt taaccaccgc gacctctttc agcgcaggcg
    11761 cgggcggtac gctgagcggc ggttcatccc ctttctttat ccacgcggtt tcactgccaa
    11821 cgcgcttttg ctgagcatct ataaacaaca acgccgcttt taagccactc agagaaatag
    11881 tctgattgcc gtcgcgtaag gtgatcgccc tcccttcctg aatcatctgc aaaaacgcgg
    11941 tgatggttgc cgtatcatcg gttactaata accatggtga aatccgccac ttgtcaccac
    12001 ttaacgccaa cggctcacca tctaacagca gccgtggcgc tatctcccct tcagacgcat
    12061 ccggcgactt caacccgccg cgctcaatac gtaaaacggc atcggtatgc gccccggcgc
    12121 tgcggctcag ggtcatcacc agtccgttat gatcgcccgt attacgcgcc acgcagaagt
    12181 tttggttatt acaggtgacc tgccagtcgg aaaacgcccg ttgcgctggt gccgcccaca
    12241 ccaaagacgt cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg
    12301 gtacgacccc agaagttcaa acaaacagca caagtcgtat cttcgttgcg caggtagcgc
    12361 ggctcaatcg gatttatcgg attgagccaa taataaacaa cgaaatatta ttgttatttt
    12421 tcagtccatt aaatgtgaca tttgcaaata gttaagcaat aacaccgtct taccgtcacg
    12481 tatctcgcca gttttgatca tctccagcgc cttgttgaac ggcagctcga gcacttcaat
    12541 atcttcatct tcgacaccac cgccggcgtt agcgcgctgg ctgtcactgt attcggcgat
    12601 aaagaagtgg attagctcag tcacaccgcc tggcgacata tacagttcaa ataatttgcg
    12661 cacttcgcca acttcataac ccgtctcttc aatcgcttct ttacgaatac acacttccgg
    12721 ttcgtcgtta tccagcagcc cggcgcaggt ttcaatcagt tgcccgcttt cattgccatt
    12781 aacccaggta gcgacgcgga actggcgaat cagaaccacg gtctttttct tcgcgttgta
    12841 caggaggatc gttgcaccat taccgcgatc gtaaacttca cgtttatggc ggataacttc
    12901 gccatctttg cgcgtgagat cgtaagtaat gttgtgcagg gtgaaatagt tatcggagag
    12961 aattttgtct ttaatgaggg tgatttgttg cgtcataccg actccacagc gcgaaatgaa
    13021 caattatctt acgctgtgaa gccgggtttg tctgcgcagg ctatcagtga gatttgactg
    13081 attttacccc cagccagtca ataatcccct gtgccgcatg acgtccttct gccattgcgg
    13141 taaccaccag atccgcacca cgcacggcgt caccaccggc gaagattttc ggattggtgg
    13201 tctggtaacg gtactggctt tccacatccg cgatgatgcg gccccatttg tctaccgtta
    13261 caccgtgcga ctccagccac ggcatcccgt gcggattgaa gccaaacgcc ataatcaccg
    13321 catcggctgg catgacaaat tcactgcctt ccaccggcac tggacgccga cgcccctggg
    13381 catccggctc gccaagacgc gtgcgcagga aacgaatccc gcagacgtga ccttgttcat
    13441 tcagttcaag cgccaccggc tggacgttaa attcgaagtt ggccccctct tcacgggcgt
    13501 tcttcacctc tttcttcgag cctggcatgt tagcttcatc acgacgataa gcgcaggtga
    13561 cgttactcgc gccgtggcgc agcgcagtac gcacacagtc catcgcggtg tcgccgccgc
    13621 ccagtaccac gacgttaagt ccggcagtat tgataaacgg ctcttccggt agctcttcga
    13681 gtcccatcac ctgtttagta ttggcgatga ggaacggcag cgcgtcataa acgcccggcg
    13741 catcttcatt gggtaaaccc gctttcatgg agcggtaagt gcctacgcca acgaagaccg
    13801 cgtcgtattg ttccaaaagc gaattcaaag agacatcttt acccacttca caattgagtt
    13861 cgaagtgaat ccccatcgcg ctgaagattt cccgacggcg tgccagcagg gatttatcca
    13921 gtttgaaaga aggaatgccg aaagtgagca agccaccgat ttctggatgg cgatcgtaca
    13981 ccgtcacctc tacgccattg cgggtcaaaa catccgcaca ggccagccct gccggacccg
    14041 caccgataat cgccacccgc ttgtccactt tggtgacatg gcttaagtca ggacgccaac
    14101 ctttcgccaa cgcctgatct gaaatgtagc gttcaatgtt accgatagtt actgcgccgt
    14161 gctcatcgcg aatagtacag gcaccttcac acaaacggtc ttgcgggcaa acgcgtccgg
    14221 taatttccgg cagggtgttg gtctggtgag aaagctcgac ggcggcgtcg atgtttccgg
    14281 ctttcaccag ttcaatccac tgcggtatat ggttatgcag cgggcaggtc cattcacaaa
    14341 cgctgtgctc gccgcactta aggcagcgcg aggcttcccg ttgtacctga tcggcacgga
    14401 atggcagata aatttcatca aaaccggttt tgcgcgcttc aatcgccagt ttatctggct
    14461 cgccacgcgc gggcgttgcc tgcatttgct cgactttact cattaccggc atttcttgcg
    14521 ccgcggtact ggcatgccac ggttgatgtt cctgacgtgc ggtacgcaag cggcgggatt
    14581 tcgccatgcc tgagagtgcg gcgtcagtga ccagttgcag cgcgtccgct gggcaattct
    14641 cgacacaagc aggaccgttt tcgcgccctg cacaaaggtc gcatttatgc gccgtggctt
    14701 ttacttttcc tgccgcgacg ggggtcagga cgatttgcat cgtaccaaat ggacaggcca
    14761 ccacgcagga tttacagcca atacactttt gctgattgac ctgaatgctg tcatcaacgt
    14821 ggctgattgc gccattaggg cagctacggg cgcagggcgc atcttcacaa tggtgacagg
    14881 tcacggcact acgttgctgc tgatgtttga taaccgtaat tcggggatga aaatggtgtt
    14941 ggctcaggac atgttgctca tcattgtgag ccatgacaca ggcgatttca caagcatgac
    15001 aacccagaca ttgctgactg ttggccataa taaaacgatt cataacgacc ttcttttttg
    15061 gttgtaaaaa ccttattctt tatatgagtg ttgttattac ccgacttaca ggggaatcgg
    15121 caatgttcat gtgcccagaa taagtaacta tttcgctata aactgtggca gatcaaataa
    15181 tcccatcact gactaaattg cgtttcagcg aattggaaca ttaatgattt tttgtggaga
    15241 agacgcgtgt gattgttaaa cgacccgtct cggccagtct ggcccgggcc tttttttaca
    15301 ttgtgctgct gtcgattctt tccacgggta tcgctctgct aacgctggcg agcagtttac
    15361 gcgacgctga ggctatcaat attgccggat cgctgcgtat gcagagttac cgcctgggct
    15421 acgacttgca aagtggcagt ccacaactca atgcacatcg ccagttattt cagcaggcac
    15481 tgcattcacc ggtattaacc aatctcaacg tctggtatgt gccagaggca gtaaaaaccc
    15541 gctatgcgca tctgaatgcc aactggctgg agatgaataa tcggctcagc aagggcgact
    15601 tgccgtggta tcaggccaat attaataatt atgttaatca gatagacctg ttcgtgctgg
    15661 ctttacagca ctacgccgaa cgcaaaatgc tgctggtggt ggcgatctcc ctggctggcg
    15721 gcatcggtat tttcacgctg gtctttttta ctttgcgccg catacgccat caggtggttg
    15781 ccccgctgaa tcagctggtt accgccagtc agcgtattga acacgggcag ttcgactcgc
    15841 cgccgctgga taccagcctg ccgaatgagc ttggcctgct tgcaaaaacc tttaaccaga
    15901 tgtcgagcga gctgcataaa ttgtaccgtt cgctggaggc gtcagtagaa gaaaagaccc
    15961 gcgatctcca cgaggccaag cgtcgtctgg aggtgttgta tcagtgttcg caggcgctaa
    16021 acaccagcca gattgatgtg cattgtttcc gccatatttt gcagattgtt cgcgacaatg
    16081 aagcggctga atatctggag ttaaatgtcg gtgacaactg gcggattagc gaagggcagc
    16141 caaacccgga attgccgatg cagattttac cggtgacaat gcaagagacg gtttacggcg
    16201 aactgcactg gcaaaatagt cacgtttcat catcagaacc gctgcttaac agtgtttcgt
    16261 cgatgctggg acgcggtttg tactttaatc aggcgcagaa gcattttcag cagttattgt
    16321 tgatggaaga acgtgcgacc atcgcccgcg aattgcacga ctcgctggct caggtacttt
    16381 cttacttacg tatccagttg acgttactga agcgttcgat accggaagat aacgccaccg
    16441 cacaaagtat catggccgat ttctcccagg cgctgaatga tgcttatcgg cagttacgcg
    16501 agctgttaac tactttccgc ctgacgctgc agcaggcgga tctcccctcc gcgttgaggg
    16561 aaatgctgga tacgttacaa aatcaaacca gcgccaaact gaccctcgac tgccgtctgc
    16621 caaccctggc gctggatgcg caaatgcagg tgcatttgtt gcaaattatt cgcgaagcgg
    16681 tgctgaatgc gatgaagcac gccaacgcca gcgaaatcgc cgttagctgc gtcaccgcgc
    16741 cggatggcaa tcacacagtc tatattcgcg acaacgggat tggcatcggt gaaccgaaag
    16801 aacccgaagg ccattatggt ctgaatatca tgcgcgaacg cgcggaaaga ttgggtggaa
    16861 cgctgacttt ttcacaacct tccggcggcg gcacgttagt gagtattagc tttcgctctg
    16921 cggagggtga ggaaagtcag ctgatgtaat gcctcttatt gaccaaagaa tagtggcact
    16981 taaggttcag tataaaaggg catgataatt tacattaact cctttttttc tccacgattg
    17041 gctcgtacct tgccgctaca gtgaagcaag tcaagcctac aacgatacgc agaaacacga
    17101 ggtcctcttt taatggcgaa tttctttatt gatcgcccca tttttgcctg ggtgctggca
    17161 atcctgttgt gtctgacagg taccctggcg attttttcat tgcccgttga acaatacccc
    17221 gatctcgcgc caccgaatgt gcgagtgacc gctaactatc ccggcgcatc ggcccagacg
    17281 ctggaaaaca ccgtgaccca ggttatcgag caaaatatga ccggcctcga taatctcatg
    17341 tatatgtcgt ctcagagcag tggcaccggc caggcatctg tcactttaag ttttaaagca
    17401 ggcaccgatc cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga
    17461 aagttaccgc aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg cgataccaat
    17521 attctgacca ttgccttcgt ctctaccgat ggttcgatgg ataaacagga tattgccgat
    17581 tacgttgcca gtaatattca ggacccgtta agccgcgtga atggcgtcgg ggatatcgat
    17641 gcctatggtt cgcaatattc catgcgtatc tggctggacc cggcgaaact caacagtttc
    17701 cagatgacgg ctaaagatgt cactgatgcc attgagtcac agaacgcgca gattgcggtt
    17761 gggcaacttg gtggtacgcc ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc
    17821 cagtcactgc tgcaaacacc agaacagttc cgcgatatca ccttgcgggt gaatcaggac
    17881 ggttcagagg taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga aaaatacgat
    17941 tatcttagcc gcttcaatgg taagccagcc tccgggctgg gggtaaaact ggcctccggc
    18001 gcgaacgaaa tggcgactgc gcagctggtg ctcaatcgtc tcgacgagct ggcgcagtat
    18061 tttccacacg gactggaata caaggtggcg tatgaaacca cctcgtttgt taaagcctcc
    18121 attgaagacg tggtgaaaac gctgctggaa gctatcgctc tggttttcct cgttatgtat
    18181 ctgttcctgc aaaacttccg cgccacgctg atacccacta tcgccgtgcc ggtggtgttg
    18241 atgggaacct tctccgtact ttatgccttc ggttacagcg tcaacacctt aaccatgttc
    18301 gcgatggtgc tggcgatcgg tctgctggtg gatgatgcca tcgtggtggt ggaaaacgtc
    18361 gaacgtatta tgagtgagga aggactcact cctcgcgaag ccacacgtaa atcgatgggg
    18421 cagatccagg gggcactggt cgggattgcg atggtgcttt cggcggtatt tgtgccaatg
    18481 gccttcttcg gcggcaccac cggtgccatc tatcgccagt tctctattac cattgttgcg
    18541 gcgatggtgc tgtcagtact ggtagcgatg atcctcactc cggctctgtg tgccacgcta
    18601 cttaagccac tgaaaaaagg tgagcatcac gggcaaaaag gcttttttgc ctggtttaac
    18661 cagatgttta accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat tctccaccgt
    18721 agcctgcgct ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg
    18781 cgtttgccga cgtcgttctt gccacttgaa gaccgtggca tgtttactac ctcggtacag
    18841 ttgcccagcg gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat cgagaaatac
    18901 tacttcaccc atgaaaaaga caacatcatg tcggtgtttg ccactgttgg ttctggccct
    18961 gggggtaacg gacaaaacgt ggcgagaatg tttatccgcc tgaaagactg gagcgaacgc
    19021 gacagtaaga ccggcacctc gtttgccatt atcgagcgtg caacgaaggc gtttaacaag
    19081 attaaagaag ctcgcgttat cgccagcagc ccgccagcaa ttagcggtct tggtagttct
    19141 gcaggttttg atatggagtt gcaggaccac gctggagcgg gtcacgatgc gctgatggca
    19201 gcacgtaacc agttgctggc gctggcggcg gaaaacccgg agctaacccg tgtgcgccat
    19261 aacggcctcg acgacagtcc gcagttgcag attgatatcg accagcgtaa agctcaggcg
    19321 ctgggcgttg ctatcgacga tattaacgac acactgcaaa ccgcctgggg ttcgagctat
    19381 gtgaatgact ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg
    19441 tatcgcatgc tgccagatga catcaatctc tggtatgtcc gaaataaaga tggcggcatg
    19501 gtgcccttct ctgctttcgc gacctcacgc tgggagacag gctcgccgcg tctggaacgc
    19561 tataacggtt attctgcggt agagattgta ggggaagccg caccgggggt cagtaccggt
    19621 acggcgatgg atattatgga atcgttagtg aagcagttgc caaacggctt tggtctggag
    19681 tggacggcga tgtcgtatca ggagcggctt tccggcgcgc aggctccggc gctgtacgcc
    19741 atttccttgc tggtggtatt cctgtgtctt gcggcgttgt atgaaagctg gtcggtgccg
    19801 ttctcggtaa tgctggtcgt gccgctgggg gttatcggcg cgctgctggc aacctggatg
    19861 cgcgggctgg aaaacgacgt ttacttccag gtgggcctgt taacggtcat tggtttatcg
    19921 gcgaaaaacg ccatcctgat cgtcgagttt gctaacgaga tgaaccaaaa aggccacgac
    19981 ctgtttgaag cgacgctcca cgcctgccgt cagcgtttac gcccgattct gatgacttcg
    20041 ctggcattta tcttcggcgt attgccaatg gcaaccagca cgggtgccgg ttccggtggt
    20101 cagcatgcgg tgggtactgg cgtaatgggc gggatgattt cggccactat tctggctatt
    20161 tacttcgtgc cgctgttctt tgtgctggtg cgccgccgct tcccgctgaa gccgcgcccg
    20221 gaataagcaa taaaaaaggc gacatgctaa tgtgtcgcct ttttcaactt tccgattaag
    20281 aacctgctca gcgggttctt gctgtttgta ctttgtctca ggaattactt acgaagcata
    20341 acttcgataa agtctttcca gttccccagt tcacgttcaa tcataacaac ctctcttata
    20401 attatgggta ttctacggaa acaatatacc gtggtgaagc taatttactc gattgctgcg
    20461 atgactacct ccgggggaca aaccttatgt aaatactatg gtcctgcggt gataatttgt
    20521 atgtgataca cagcaa
//
LOCUS       DAZGHF010000083        20117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGHF010000083 DAZGHF010000000
VERSION     DAZGHF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.083"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            217..717
                     /gene="tssB"
                     /locus_tag="ACHK3A_003165"
     CDS             217..717
                     /gene="tssB"
                     /locus_tag="ACHK3A_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO8741216.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSGK"
     gene            752..958
                     /locus_tag="ACHK3A_003166"
     CDS             752..958
                     /locus_tag="ACHK3A_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741217.1"
                     /translation="MAAHKNISGKHPLSDEDILQAVYRVCFSPSGEINRDGVDALITT
                     LDRRISDLLDEIIHLCEFQQSAPY"
     gene            1027..2502
                     /gene="tssC"
                     /locus_tag="ACHK3A_003167"
     CDS             1027..2502
                     /gene="tssC"
                     /locus_tag="ACHK3A_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO8741218.1"
                     /translation="MSLQEEELVSSHAGQTDQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2509..2922
                     /gene="tssE"
                     /locus_tag="ACHK3A_003168"
     CDS             2509..2922
                     /gene="tssE"
                     /locus_tag="ACHK3A_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO8741219.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2926..4776
                     /gene="tssF"
                     /locus_tag="ACHK3A_003169"
     CDS             2926..4776
                     /gene="tssF"
                     /locus_tag="ACHK3A_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO8741220.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     LRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSHPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4740..5822
                     /gene="tssG"
                     /locus_tag="ACHK3A_003170"
     CDS             4740..5822
                     /gene="tssG"
                     /locus_tag="ACHK3A_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO8741221.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSDVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5847..7127
                     /gene="tagH"
                     /locus_tag="ACHK3A_003171"
     CDS             5847..7127
                     /gene="tagH"
                     /locus_tag="ACHK3A_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO8741222.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCVFTQQGNVGHGPECQWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSTDPLQALQSESLITAGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSVAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7124..7648
                     /gene="tssJ"
                     /locus_tag="ACHK3A_003172"
     CDS             7124..7648
                     /gene="tssJ"
                     /locus_tag="ACHK3A_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO8741223.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7651..8982
                     /gene="tssK"
                     /locus_tag="ACHK3A_003173"
     CDS             7651..8982
                     /gene="tssK"
                     /locus_tag="ACHK3A_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO8741224.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGILPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWAEIAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8987..9748
                     /gene="icmH"
                     /locus_tag="ACHK3A_003174"
     CDS             8987..9748
                     /gene="icmH"
                     /locus_tag="ACHK3A_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO8741225.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FYGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9757..12546
                     /gene="tssH"
                     /locus_tag="ACHK3A_003175"
                     /gene_synonym="clpV"
     CDS             9757..12546
                     /gene="tssH"
                     /locus_tag="ACHK3A_003175"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO8741226.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEINLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQALVQEIIALRQQLLGVAEDD
                     AAPLPDADTVEDTPPESESESESESESEQDNTGAVPADEADREQPEETAETVSPVQRL
                     AQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGD
                     TIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGR
                     QYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHP
                     DVLNLFYQSFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEELYPV
                     LADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDE
                     IMSRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDDAFTADVE
                     DAQNDESVTKDETVL"
     gene            12543..13286
                     /gene="vasI"
                     /locus_tag="ACHK3A_003176"
     CDS             12543..13286
                     /gene="vasI"
                     /locus_tag="ACHK3A_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO8741227.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAETSSMAGVAVAVATTP
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRVACHWAGE"
     gene            13291..14703
                     /gene="tssA"
                     /locus_tag="ACHK3A_003177"
     CDS             13291..14703
                     /gene="tssA"
                     /locus_tag="ACHK3A_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO8741228.1"
                     /translation="MAMNLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQHDALSGELAKLAQCWQSYN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSRQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAT
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14812..18246
                     /gene="tssM"
                     /locus_tag="ACHK3A_003178"
     CDS             14812..18246
                     /gene="tssM"
                     /locus_tag="ACHK3A_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO8741229.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGIYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     GLFPQALLAEPNLATESRTWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPLGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKL
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18257..19667
                     /locus_tag="ACHK3A_003179"
                     /pseudo
     CDS             18257..19667
                     /locus_tag="ACHK3A_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19633..20112
                     /locus_tag="ACHK3A_003180"
     CDS             19633..20112
                     /locus_tag="ACHK3A_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO8741230.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELYYK
                     IKLTGAKISDIHVSISHIVVDNSIQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
BASE COUNT         4739 a         5511 c         5555 g         4312 t
ORIGIN      
        1 cacaggttca cacaatctat ttaacgcttg cagaataccc ctctttacga tcgataagaa
       61 gtccttttct tatttatttt gaagtgtaga atgcggggtg atgaaggagt ggtaatttta
      121 ttttcaccgc cattagggcg gcattgccga tatttttttg cggtacaaat atgccgtgtt
      181 catggcctgt acgagataac atgacggatc acttttatga gcaaaaaatt tgaaggcagc
      241 gtcgcgcctc gcgagcgtat aaatatatcc tatgtcccga agacggacgg ccagacggct
      301 gaagtagagc ttccgcttaa catgctcgtt gtcggcgata ccggaaatac gcaggagaca
      361 tccccactgg atgaacgtca ggcagtttcc gttaacaagc acaatttcgg tgcagtaatg
      421 gctgaagcag ccattggcct caattttacc gtccccgcca cgctgaaggg aagcacaacg
      481 gacgatgaac tgaatgtggc gctgaatatc aagtcactgg atgatttttc accggacagc
      541 gtggcccggc aggttccgga agtgaataaa ttgctggaac tgcgtgaagc actgactgcg
      601 ctgaaggggc cgatgggcaa ccttccggca ttccgtaccc agctccaggc attgctggaa
      661 aatgaggagt cccgcgagca gttgcttaaa gaaatcggcc aggtgagtgg gaaataacca
      721 ccactatttc attaaacagg gttatccaga catggcggca cacaaaaata tctccgggaa
      781 acaccccctc tctgacgaag acattttgca ggcggtttac cgggtatgtt tcagcccgtc
      841 cggtgaaatc aaccgggatg gcgtggatgc gctgattaca acactggatc gccgtatcag
      901 tgatctattg gatgagatta tccatctttg tgagtttcag cagagcgcgc cgtactgacg
      961 gcgcgtactt cactgacggc gaaaacccgg tttcagaaac acagctaaaa cagaaggatt
     1021 ttcagtatgt ctttacagga agaggaactc gtttccagtc atgccgggca gactgatcag
     1081 gcgtcgtcgc tgcttgatca aatcatggca cagacccgta ttcagcctgg ttcggaaggt
     1141 tatgacgttg cccgccaggg tgtgacagcc tttattgcca gcatcctgca atccactgca
     1201 tctgccgaac cggtcaataa actggccgtg gacagcatga ttgcagatat agatgaacgc
     1261 atcagccgcc agatggatgt catcatccat gcgcctgcct ttcagcaggt cgagtcgttc
     1321 tggcgctcac tgaaaaccat ggtggatcgc gttgatttcc gtgaaaacat caaggtcaac
     1381 gtgcttcatg tcacgaagca ggaacttctg gaagactttg aatttgcccc agaaattatt
     1441 cagtccggat tttataaaca cgtttattcg tccggattcg gacagtttgg tggtgaaccc
     1501 atcgctgccg tgctgggcgc ttatgagttc aaaaataccg ctcctgacat gaaactgctg
     1561 caatacgtca gtgccgtggg cgcaatggcg cacgcaccgt tcctgtcctc cgtttccccg
     1621 gagtttatgg ggctgaactc gtggaccgaa ctgcctaata tcaaagatct ttatgccatc
     1681 tttgaaggtc cggcctacac caaatggcgc gccctgcgtg actcggaaga ttcgcgctat
     1741 ttagggctta cggcaccccg cttcctgctt cgccagccct attcaccaac agataaccct
     1801 gttaagaact ttaactatta cgaagatgtc agccagaacc acgaagatta tctgtggggt
     1861 aatacggcct ggatgctggc atgcaatatc gcagacagtt ttgccaaata ccgctggtgt
     1921 ccaaacatta tcggtccgca aagcggcggc gcagtgaaag atctgccggt gcatctgttc
     1981 gaaacgatgg ggcagattca ggccaaaatc ccaaccgagg tgctggtcac cgatcgccgc
     2041 gaatttgaac tggctgaaga gggttttatc acccttacca tgcgtaaaga ctctgataat
     2101 gcagcctttt tctctgcaaa ctcggtacaa aaaccgaagc actttccggg aaaagacgca
     2161 gaaaccaact ataagctggg tacgcagctt ccttatctct tcatcatcaa tcggttagcg
     2221 cactacatca aggtgttgca gcgtgaacaa ctggggtcat ggaaagaaag gagcgactta
     2281 gaaagagagc ttaatacctg gatccgtcag tatgttgccg accaggaaaa cccgcctgca
     2341 gacgtgcgca gccgcaaacc tctgcgcgct gccaaagttg aggttatgga tgtagaaggc
     2401 gaacccggct ggtaccaggt tgcgctaagc gtgaggcctc attttaaatt tatgggggca
     2461 aattttgagc tttccctggt tggccggtta gacagggagt aacacaccat gataccggat
     2521 cgggaaagaa ccacaggttc tctttttgag cgtatggaag cctcttcagc cagaaaccgg
     2581 cagggaggca gtattcattc gctcaggcaa tccattcgcc agaacctacg aaacattctt
     2641 aatacccggt ccggtagttg ccggggtgcg cctgagctgg gcattgatga accggaaggg
     2701 gctgaaaact tccgcgaatc aatgagtcgg gccatcgagc aatgtataga acgctatgaa
     2761 ccccggatct cacacgctga agtgcaggca gtggtttcat ccgcctccag cccgctggat
     2821 atgacgtttc atattacagc ctgggtgaca tttaacgaaa cgcatgaggt gcttgaattt
     2881 gatatggcac cgaatggcag ccagcattac cgggtggact gacaaatgga atttgaagaa
     2941 cgctatttcc gggaagaact cgattacctg cgccagctta gcaagctgct ggcaacggaa
     3001 aaaccccatc tggcccgctt cctggccgaa aaagatgcag atccggatat tgaacgcctg
     3061 ctggaagggg tggcttttct taccggcaat ctccgccaga aaattgagga tgaattccca
     3121 gaactgacgc acgggcttat taagatgctc tggcctaatt acctgcgtcc ggttccggca
     3181 atgaccctta ttgaatatac gccggatatg gataagtctt ctgtaccggt gttaatcccc
     3241 cgtaatgagc agtttacaac caacgccggg gaaatcagag ttgatgaagt gctgccctct
     3301 gatgctaaaa aggaggagcc tcctccctgt accttcaccc tctgccggga tatctggctg
     3361 ctgcccgttc gcctggagca gattgaaaac cgcagtacga cccgtaatgg tgttatcaac
     3421 atcacctttt cggtcgcacc gggaacggac ttccgcacgc tggatctgaa caaacttcgc
     3481 ttctggctcg gcaatgacga caactatacc cgtgaccagc tttatttatg gttctgcgaa
     3541 tacttgcagg gtgctgacct gactgtgggt gaacagcatc ttcgcctgcc tgagtttatg
     3601 ctaaaagctg tcggttttga gccgcaggat gccatgctgc cctggccgaa aaacgtccac
     3661 agcggctacc ggatccttca ggagtatttc tgttaccccg atgcgtttct cttttttgat
     3721 ctttgtggtt gtccggcttt gcctgacgga ttgcaggcgg aattctttac cctgcaactg
     3781 cgtttttcgc acccattgcc cgtggacatc cggttgcgcc gcgactccct gcgcctgtat
     3841 tgcgcgcctg ccattaattt atttatccac catgcagaag ccatcacgct ggacaaccgg
     3901 cgggcagact atccgctggt tcccagccgc cattatccgg aacattacga tgtcttttcc
     3961 gttaacggcg tgataagcca ggtgcaggat atgttcagga aaaaagatct gggtcgtcct
     4021 gtttcgacgc aggccgcgcg ccagtggcct gcctttgaaa gtttcagcca tcagatggaa
     4081 tacagccgga agcgggaagt ggtgtactgg catcaccgga ccaaaacatc cctgttccat
     4141 cgcggctttg atcataccct tgcttttata catgctgatg gcagttatcc gtcagacgaa
     4201 tcgctgctca gtaatgaagt ggtttcggta tcgctgacct gtaccaaccg tgaacttccg
     4261 tcacaaattc gttccggcga tatcaccggc acaaccggta aaaatgcggc tgttgcttca
     4321 tttcgcaaca ttacccgccc gacgcaaccg ctctggccgg tcattgatgg cagcctgcac
     4381 tggtccctgc tctccgccat gaacctgaat tatctgtcat tactggatac ggacgcgctg
     4441 aagcaggtca tcgccaactt tgatcgccac gcaatccatc atccgcagac ggcgcggctg
     4501 tcacaacaaa agctggatgc cattgagcgt ctggagaccc gccctgtcga tcgcctgttt
     4561 acgggcattc ccgtccgggg gctggcctcc acgctgtatc tgcacccgga gccgtttgtc
     4621 tgtgaagggg aaatgtatct gctcggtacg gtgctttcgc attttctgtc gctgtacgcc
     4681 agcgttaact cattccacat gctgaccgtt gtgaacacag aaagccagga gacatggaaa
     4741 tggacggaaa gaaccgggca gcatcctctt atctgagtcc gggaaatccc ccggcagata
     4801 aagagcagaa tgatccgctg gcacaggttt ttcataacgc ctgctcttac aatttttttg
     4861 cgatggcgga gctgttgcac cgcctggcaa agggtgaaaa ggggacgcca gaattatccc
     4921 tgcgtgacga tccggcacag gaaaccctgc gtttcagcgc cgatgccagc cttgccttcc
     4981 cgtgcagtga tatcagtgcg ctgaaacggg atacgtcagg ggcattcagg atgaccacca
     5041 cctttatggg gttacagggg agccagtcgc ctctccccgg ttattacctc gatcacctgg
     5101 cctggaaagc ggtacatgaa caaagtccgg taggtgattt tctggatatg ttcagccacc
     5161 gcctgaccca gtttgtctgg catatctggc gtaagtaccg ctatcacatc agttttcgta
     5221 acggcggtgt ggatgcgttc tcgcagcgca tgtactccct tgtcgggctg ggccaccgcc
     5281 agcttcgcga taagctggcg attaatcaca gcaaaatgct ggcgtattcc gggatcctgg
     5341 caaatccggg acgatccccg gaaataatct gcggcctggt gtcccactgc tttgatttgt
     5401 cagacgtgac gctgcaaaac tggcaacgtc gcaaggtgga tattgaaccg gatcagcaaa
     5461 acagtctggg gagctattca cttaaaaatg gcgaaaaact ggcgggaaga tcggtactgg
     5521 gcaactttgt tctgggtaca cgggtgcccg atctcagcgg aaaattccag ctcagtatta
     5581 ccagtctgac ccgaaaacag ttcctctctt ttctgccgtc cggcgaaaac ttcctgccac
     5641 tgacgatgtt tgtgtccttc atcctgcgcg atcagcttgc ctgggactta catctggggc
     5701 tggcgccgga gcaggtgggc gcaatgcgcc tgggcgataa caaaagtgcg ctgctgggct
     5761 ggaccagctt cctcggcaca ccggaagaac gaccctcagt caccatcagg gtacggtcgt
     5821 aaattatgat aatcacggag cagaatatgc cggaagagaa actacagacg ctttcattgc
     5881 aggtcattaa cggcagtgag ctggaaagcg ggcgggcggc gcgctgtgtg ttcacacagc
     5941 agggaaatgt cggccatggc cccgaatgcc aatggtcggt acaggatcgt cagcagagca
     6001 ttccggctca ggcttttacc gttatcctgc acgatggcac attttgtctg cgcccgcaga
     6061 cggcacaact gtggctgaac caggcaaaag tcacagcaac atcagacctg atacagctgc
     6121 gccagggcga tgagatccag atagggcgtc tgatggtgag ggttcatctg aaccggggag
     6181 atatccccca ttacgatgag gaaatggcca ctcccgaaac catcgtcacc aatcgcgata
     6241 tgctcacgga taccctgctg tcaacggagg gtgcgccaca ctatccggga atgactcacc
     6301 ggcatcagct tgcagacacc gtggtaaacg gtttttctac cgatccactc caggcacttc
     6361 agtccgaaag cctgattacc gcgggcgatc cgctttcagg cattgcggct gtccggccat
     6421 cggcaccgct gtccgatccg gcaagtaatg gggggatcaa tactccgttt atggatctgc
     6481 cgcccattta tgccagcccc ggcgatcgta atgatgacgt ctctgtggca gaaatggcgc
     6541 aacgccacct tgcggtcacc cccttactgc gcggtctcgg cggttcgctt accgtgagca
     6601 attccgacga tgcggatgat tttctggaag aggccggacg cacgttacag gccgcaataa
     6661 aaggtctgct cgatttgcag caacagcgta acagcctctc agacaaacat ttgcgcccgc
     6721 tggaagataa cccgctgcgc ctgaacatgg attacgccac cgcgctcgac gtgatgtttg
     6781 ccgaaggtaa aagtccggta catctggcgg ctcccgccgc tgtcagtgaa agcctgcgca
     6841 atgtccgcca ccacgaagaa gctaaccggg cagcgattgt ggagtcactt cgtgtcctgc
     6901 ttgacgcttt ctcaccacaa aatctgctgc gccgctttgt gcagtaccgc cgcagccatg
     6961 aactgcgcca gccgctggat gatgccggag catggcaaat gtacagccat tattacgaag
     7021 aactggcctc cgatcgccag caggggtttg agatgctgtt taacgaggtc tacgcccagg
     7081 tctatgaccg ggtgcttcgt gaaaaacagc gggagccgga agcatgaaca ggaaggcgtt
     7141 tctggcctgc gtgctgatgt gcgtcatatt aaccggctgc gaaacggcga aaaaaatcag
     7201 cgaggtgatc aaaaacccgg atatccaggt cgggagcctg aaggagcaac cttcggaaat
     7261 aaccgtcacc ctgctgaccg aaccggacac caataccaat gcggagggtg aatcagccgc
     7321 cgtggatgtg caactggtgt acctgactga cgactcaaaa ttgctggcag ccgactatga
     7381 ccagatagcc agtacgccgc taccggatgt gttgggtaaa aactatatcg accaccagga
     7441 tttcaacctg ttgccggaca ccatcaaaac cctgccgccg gtaaagctgg atgagaaaac
     7501 gcagtttatt ggtgtcgtgg cctatttctc cgatgaccag gccactgaat ggaaacaaat
     7561 cgagacggtg gaaggaaccg gccaccatta ccgcctgctg gtgcatgtac gtcaaagcag
     7621 catcgaaatg aaaaaagagg atgaataatt atggcaacca cccgcaacaa ggtgatgtgg
     7681 caggaaggga tgctgatgcg cccacaccat ttccagcaac agcagcgtta caacgactac
     7741 ctggataacc agcgtttccg ggccatgaat gatttatcct ggggatttac cgaactcacc
     7801 ctcaacaatg aactgctggc gcagggtaag atcatgattg acagcgcgtc aggcatactg
     7861 cccgacggca ccgtcttttc tatccccgac caggacgcac tgcccgatcc gctgcacccg
     7921 caaaattttc cggacgaaag aagccgcaat atttaccttg ctctgccggt cgccagtgat
     7981 gtgagaaatg aaatcagcga cgggcggcga atcgggcgtt accggctgaa ttatgccgat
     8041 gtccgggatc tgcattcaga agaaggtgac gcgcgaacac tgacgctggg tcaactgacg
     8101 ccgcgcatta tgagcggtgc agaagatatg agcgcctata ttacgctccc gctttgccgt
     8161 atcagcgatc gccatgccga cggctccctg acgctggatg acgattttat cccctcctgc
     8221 cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca aggggccatt
     8281 ggcggacggg caagcgatct ggcaaaccgc attggctctc cggcgcagag cggcatcgcg
     8341 gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg gtttacccat
     8401 cgcgctcgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc cgggttgctg
     8461 ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga tgtgtatgat
     8521 catcatgacc tgaccaaaac atttaaaacg ctgttaccgg aagtcaaacg ggcgctgcat
     8581 accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg tatctggcag
     8641 gccgatattc atgacacaga actgctgcaa tctgccacct ttgtgctggc cgtggcggca
     8701 aacatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat ttcctcgccg
     8761 gaaaaaatcc gcaatatggt cagtgtgcag atacccggca ttccattgcg tgccctgatg
     8821 gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact cgacaaaagc
     8881 ggccaggcct gggctgaaat agctgccgcc ggggccgttg cactgcatgt ttccggcagt
     8941 ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg atgaggggtc
     9001 actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcgact accatctcgc
     9061 gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac tcggtatggt
     9121 aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg ctcaggtggt
     9181 gacggatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac cgggcgtgat
     9241 tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg gtaacggctg
     9301 gtcgaacaag aacgagtgga tcaaacagtc attgctggtc catttccaca acgaagcctg
     9361 gggtggagag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca aacgttacca
     9421 ggatctgctg gaatttctct ggctctgttt ttctctggga tttcgcgggc gctacaaagt
     9481 ggcagcacag gatcaggggg aatttgagca gatctaccgc cgcctgtacc acgtcctgca
     9541 taaactacgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa cccagggcgg
     9601 gcgttatcag ctcatcagtc gcctgaccat taaacatatt ttttatggtg gcgttgtcgt
     9661 gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc aggacatcct
     9721 gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga tcttcccacg
     9781 ctggtaaaac ggcttaacct gttctcccgc caggcgctgg agatggccgc ctctgaatgt
     9841 atgagccagc aggcagcgga aattaccgtc agccatgtgc ttattcagat gctcgccatg
     9901 ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga agagttgcgc
     9961 caggcgctga cggtggagaa ctacacaact gcccgttctg cggacagcta cccggcgttt
    10021 tccccgatgc tggttgagtg gctgaaagag ggctggctgc tggcgtcggc tgagatgcag
    10081 cacagcgaat tgcgtggcgg cgtgttactg ctggccctgc tgcattcgcc gctgcgttat
    10141 ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct gcaacaggac
    10201 tttgtacagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga cggtaaaggc
    10261 gcaggcacac tgacggacgc tgctgacacc ctgcttgccc gctacgccaa aaacatgacc
    10321 gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga aatcaacctg
    10381 atgatcgaca ttctctgccg ccgccgcaag aacaacccgg tggtggtggg cgaagcaggc
    10441 gtgggtaaaa gtgccttaat cgaagggctg gcgctgcgca tcgtggcagg ccaggtgccg
    10501 gacaaactga aaaacaccga tatcatgacc cttgacctgg gcgcattgca ggccggggcg
    10561 tcggtgaagg gcgaattcga aaaacgcttc aaggggctga tggcggaggt catttcctct
    10621 ccggtgccgg tcattctgtt tattgacgaa gcacataccc tgattggcgc gggcaaccag
    10681 cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg cgaactgaaa
    10741 accatcgccg ccaccacctg gagcgaatac aaaaaatact tcgagaaaga tgccgctctg
    10801 tcgcgccgct tccagttagt gaaggtcagc gaacccaacg ccgctgaagc caccattatt
    10861 ctgcgtggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga tgatgacgcc
    10921 ttgcaggccg cagcaacgct gagcgaacgt tatctctccg ggcgtcagtt gccggacaaa
    10981 gccattgatg tgctggatac cgcctgcgcc cgcgtcgcca ttaacctttc ctcgccgccg
    11041 aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat tcgccagctt
    11101 gagcgcgagc ttcgcatcgg gctgcgtacc gacacatcac ggatgaccga ggtgctggtg
    11161 cagtatgatg aaacgctgac ggcgctggat gaactggagg ccgcctggca tcagcagcag
    11221 gcgctggtac aggagattat cgcgctgcgc cagcaattgc tgggcgtggc agaggacgat
    11281 gcggcgccgt tgccggacgc agataccgtg gaggatacgc cgccagagtc agagtcagag
    11341 tcagagtcag agtcagagtc agaacaggat aataccggtg ccgtaccggc tgatgaggcc
    11401 gacagagaac agccggaaga gaccgctgaa acagtttccc cggtacagcg gctggcccag
    11461 ctcactgccg aactggacgc cctgcataac gaccggttgc tggtctcccc gcacgtcgat
    11521 aaaaaacaga ttgcggcggt gattgccgaa tggaccggcg tgccgctcaa ccgcctgtcg
    11581 cagaatgaaa tgtcggtcat caccgacctg ccgaaatggc tgggcgacac catcaaaggc
    11641 caggacctgg cgattgccag cctgcataag catctactga ccgcacgcgc agacctgcgt
    11701 cgtccgggac gcccgctcgg tgcgttcctg ctggctggcc ccagcggtgt gggtaaaacc
    11761 gaaaccgtcc tgcaactggc agagctgctc tacggcggtc gccagtacct gaccaccatc
    11821 aatatgtccg aattccagga gaaacacacc gtctcgcggc tgattggttc gcctccgggc
    11881 tacgttggct acggtgaagg cggcgtactg accgaagcga ttcgccagaa accctactcg
    11941 gtagtactgc tcgatgaagt ggaaaaagcg cacccggatg tgctcaacct gttctaccag
    12001 tcgttcgata agggcgaaat ggcagacggt gaaggccgcc tgattgactg caaaaatatc
    12061 gtcttcttcc tgacgtccaa cctcggctac caggtaatag tcgagcacgc cgatgacccg
    12121 gaaaccatgc aggaagaact gtatccggtg ctggccgact tcttcaaacc tgccctgctg
    12181 gcgcgtatgg aagtggtgcc gtatctgccg ctgtcgaaag agacgctcgc caccattatc
    12241 gccggaaaac tggcccgtct ggataacgtg ctgcgcagtc gctttggtgc agaagtggtc
    12301 attgaaccgg aagtgacgga cgaaatcatg agccgcgtca cccgcgcgga aaacggcgcg
    12361 aggatgctgg aatcggtcat cgatggcgac atgctaccgc cgctctcgct gctgctgttg
    12421 cagaaaatgg cggctaacac ggcgattgcc cggattcggt tgtcggcggt ggacgacgca
    12481 tttacggcag acgtggaaga tgctcagaac gacgagtccg tcacaaagga tgaaacggtt
    12541 ttatgagcag gttgcgcgtt ttccggcaga caggatggtt tatcgcgggc ctgatgacgg
    12601 gcttacccgc cactgccgcg cccgcagaaa catcgtcaat ggccggtgtg gcggtcgccg
    12661 tcgccaccac gcccccgccg gatgcgacag ccacgttaca ggccatgcag tcctgtcggc
    12721 gggagtccgc ggcgctggag cggcttgact gctatgaccg cctgctggca ccgctgtccc
    12781 cgtcagggtt tgacggggcg ctggtcaaag ccggttttgt gggcgaggcc tggacccgcg
    12841 ccacagagca ggaaaaacgc cgcgagggca acaccacaga actgctggtg acgcaggtgc
    12901 cgggggagcg cccgacggtg gtgataacca ccccggctat cggccatgtt ccgccgcgcc
    12961 cggtgctgat gttcagttgc gtggacaata tcacccggat gcaggtggcg ctgatgcacc
    13021 ctctggatgt ccatgatatc gccgtcactc tgaatgccga cagccgggca ttgcgtagcc
    13081 actggtttgt gcgcgagaac ggcaccctgc tggagtccag ccgggggctg tccgggattg
    13141 atgaaattaa gcaactgttc ggcgcaaaaa cgctgacagt ggataccggc gcagataacg
    13201 ccgcaggaaa gctgaccttt aacattgacg ggctggcaag ggcgattgcc cccctgcgcg
    13261 ttgcctgcca ctgggcggga gaataaaaat atggcaatga acttacgcga tccgaatgtc
    13321 tggatatcgc acctgctgga aaacctgccg gaagaaaaac tggcatcggc gctgaaagat
    13381 gacaacccgg actgggagta tatcgacggc gaaatcgtca aactgggatc tctggcccac
    13441 agtcaactgg atatcccgga actccagcgc cgggggctgg tcattctggc ttcggagagt
    13501 aaggactttc gtctgctggc gcatctgctg agaaccctgc aacacgccgg tgatcctcat
    13561 ctcgcattgc gcctgctggc gctgtatgtg gagcattact ggtcggtggc tgcgccgcag
    13621 aatatggcac ataaaaaacg ctttgccagc caggtcatta aacgctttga aacaggtatt
    13681 gaaggctttt cacagaacgc cgccacagcg cagcacgacg cgctgtcagg ggaactggcg
    13741 aaactggcgc agtgctggca gtcatataac gccccggaac tggcactggc taccgatgat
    13801 ctttttgccc tgtaccagcg tgcgtttaat cgtgcggcac cggctcctgc ccccactccg
    13861 gcggcctccg atagttcacg acagaccacc gccacgtctg aaagcggcgt gacgcaaccc
    13921 agtgctccgg ctccccaaat cgccatcgac agtcacgacg acaaagcctg gcgcgacacg
    13981 ctgttaaaag tggcggctat tttatgtgaa cgccagccgg actcgccgca gggctatcgc
    14041 ctgcgccgcc atgccctgtg gcaatccatc accagtacac cccaggcgga aagcgatgga
    14101 cgtaccccat tggctacggt ctctgccgat atggtggcgg attaccagtc ccggcttgcc
    14161 agcgcggata tggcgctgtg gcagcaggtt gagaaaagcg tattgctggc tccttactgg
    14221 ctggacggtc actgtctttc tgcacagacg gcactgcgtc tgggttacaa acaggtggca
    14281 gacaccatcc gcgatgaggt catccgcttc cttgagcgtc tgccccagct taccgggctg
    14341 ctgtttaatg accgcacacc gtttctcagt gagcagacga aacaatggct ggcagcttcg
    14401 cccgacggca aagttgcacc ggttgcgcaa atcggtgagg aatcgcaggc agccagagcc
    14461 tgttttgctg ggcagggtct ggaggcggcg ctgcgatatc tggacatgct acccgaaggc
    14521 gatccccgcg atcagtttca ccgccagtac cttgccgcac agttgacgga ggaggcgggg
    14581 ctgatacagc ttgcgcagca acagtaccgg atgttgttga tgatagggag tcagatgatg
    14641 gtgtctgact gggagccatc attacttacg cagcttgaac aaaaattcac ggcagaacaa
    14701 taaaagagaa caggagtttt tgtgttcaga ttaccaacac cccgactact cagcggactc
    14761 aaatcagccc tacgcccggc gatgccccgg tttaaagtct ccgctttctg gctgctgata
    14821 ctggcgtgga tttttctgct ggtatggatc tggtggaaag gcccgacatg gacgctgtat
    14881 gaagaacagt ggctcaaacc actggcgaac cgctggctgg caacggcggc atgggggatt
    14941 atcgccctag tgtggcttac cgtccgggtg atgaagcgcc tgcaacagct tgaaaaaatg
    15001 caaaagcagc agcgcgagga ggccattgat ccgctcagtg tggagctgaa cgcccagcag
    15061 cgttaccttg accgctggct gctgcgcctg caacgccatc tcgacaaccg ccgtttcctg
    15121 tggcagttgc cgtggtatat ggtcatcggc ccggcgggca gtggcaaaac gacactgctg
    15181 cgcgagggtt ttccgtccga cattatttat gccccggagg gcgcacgcgg cgcagaacaa
    15241 cgcctgtacc tcacgcccca tgtcggtaaa caggcggtga tctttgatat cgacggcaca
    15301 ctctgcgctc ccgctgatgc ggatatcctg catcgccgtc tgtgggaaca tgccctcggc
    15361 tggctgaaag aaaagcgcgc gcgccagccg ctgaacggga ttattctgac actggattta
    15421 cccgatctgc ttaccgcaga caaacgccgc cgcgagcatc tgttacagac gctgcgcagc
    15481 cgcttgcagg atatacgcca gcatcttcac tgccagttac cggtttacgt ggtactcacc
    15541 cggcttgatt tattgcaggg ttttgccgcc ctgttccagt cactgaacag acaggatcgc
    15601 gatgcaattc taggggtcac gttcacccgc cgcgcccatg aaaatgatga ctggcgaaca
    15661 gagttgaatg ctttctggca gacatgggtg gatcgaatga acctagcgtt gccggatctg
    15721 atggtcgctc agactcacac ccgcgcgtct ttatttagtt tttcccgcca gatgcaggga
    15781 agccgtgaac cgctggtgtc actgcttgag ggtctgcttg acggcgaaaa tatgaacgtg
    15841 atgctgcgtg gtatctatct cacctcttcg cttcaacgcg gacagatgga tgatattttc
    15901 acccagtctg ccgcccgcca gtaccggctg ggcaataacc cactggcgtc ctggcccctg
    15961 gtggacaccg cgccttattt cacccgtggc ctgttcccgc aggcattact cgcagagcct
    16021 aatctggcaa cagaaagccg cacctggctg atgcgttccc gtcgccgcct aacggttttc
    16081 tctaccacag gaggcgtggc ggcactgctg ctcatcaccg gctggcatca ctattacaac
    16141 ggtaactatc agtccggcat caccgtgctt aagcaggcaa aagcctttat ggatgtaccg
    16201 cctccgctgg gggaagatga ttacggcaac ctgcaactgc cgctgctgaa cccggtacgc
    16261 gatgccacgc tggcctatgg cgactggggc gaccgcagcc gtctggccga tatgggactg
    16321 taccagggac ggcgtatcgg gccttatgtg gaacaaacct atctgcaact gctggagcag
    16381 cgttacctgc cttcgctgtt taacgggctg gtcaaagcga tgaacgccgc gccgccggag
    16441 agtgaggaaa aactcgccgt gctgcgtgtg atgcgaatgc tggaggacaa aagcggacgc
    16501 aataatgagg tggtgaagca gtatatggca aaacgctgga gcgaaaaatt ccacggccag
    16561 cgcgatatcc aggcacaact gatgtcccat cttgactacg cgctggctca tactgactgg
    16621 catgcagagc gtcaggcggg cgacggtgac gccatcagcc gctggacgcc atatgacaag
    16681 ctcgtggtat cagcacagaa agaactgagc aaactgcctg tctaccagcg ggtttatcag
    16741 agcctgaaaa cgcgggcgct gggcgttctt cctgctgacc tcaatctgcg tgaccaggta
    16801 gggccaacct ttgaccaggt gtttacatct gccgatgaca acaaactggt tgttccacag
    16861 tttatcaccc gctatggcct gcaaagctat tttgtaaaac agcgcgatga actggttgaa
    16921 ctgacagcga tggattcctg ggtacttaac ctcacccgca gtgtgaaata cagtgacgcc
    16981 gaccgtgcgg aaatccagca ccagttgacc gagcagtata tcagcgacta caccgccacc
    17041 tggcgggccg ggatggacaa tctgaatatc cgcaattttg agtccatcgg acaactgacc
    17101 ggggcgctgg agcaggttat cagcggcgac cagcctttgc agcgggcgct gaccgtgctg
    17161 cgtgacaaca cacagccagg cgtcttttct gaaaaactct ctgccaaaga acgggaggaa
    17221 gccctggcag agccggatta ccagttactc acccgcctcg ggcatgaatt cgccccggaa
    17281 aacagtaccc tggcagtaca gaaagacaaa gaaagcacga tgcaggccgt gtatcagcaa
    17341 ctcaccgagt tgcaccgcta cctgctggca atccagaacg cgcctgtacc agggaaatcg
    17401 gcgctgaaag ccgtgcagtt acggcttgat cagaacagca gcgatccgat attcgccacc
    17461 cgccagatgg caaaaacgct gcctgctccg ctcaaccgct gggttggcag actgactgac
    17521 caggcctggc atgtggtgat ggtggaggct gttcattata tggaagtgga ctggcgcgac
    17581 agcgtggtga aaccgtttaa cgagcaactg gcaaataact atccgtttaa tccgcgttct
    17641 gcacaggatg cctcactgga tgccttcgaa cgcttcttta aaccggatgg catactggat
    17701 accttctacc agcagaacct gaagctgttt atcgataatg acctgagtct ggaggatggc
    17761 gataacaacg tcattattcg cgaagatatt attgcgcaac tggaaactgc gcagaaaatc
    17821 cgtgacatct tcttcagcaa acagaacggt ctgggaacat cctttgccgt ggaaacggta
    17881 tcgctttccg gcaataaacg ccgcagtgta ctgaaccttg acggtcagtt agtcgattac
    17941 agccagggcc gtaactatac cgcccatctg gtctggccta acaacatgcg cgaaggcaac
    18001 gaaagtaagc tgacgctcat cggcaccagc ggcaacgcgc cgcgcagtat cagcttcagc
    18061 gggccgtggg cgcagttccg cctgttcggg gccggacaac tgaccggagt acaggatggc
    18121 aactttaccg tgcgctttag cgtggacggt ggcgcgatga cctaccgtgt gcataccgac
    18181 accgaggata acccgttcag cggtgggttg ttcagccagt ttggtctgtc agacacactg
    18241 tactgaggag gcagggatga atagtaacgt actgacacaa actatcgtta ccggcagtga
    18301 cccgcgcggg ctgccggaat tcagcgccat tcgcgaggaa ataaacaaag ccagccaccc
    18361 gtcacagcct gagctgaact ggaaactggt ggagtcgctg gcgctggcga tttttaaagc
    18421 taacggtgtg gatttacaca ccgccaccta ctatacgctt gcacggacac ggacacaggg
    18481 actggcgggc ttttgcgaag gtgcggaact gctggcggca atggtaagcc acgactggga
    18541 taagttctgg ccacagggcg gtccggcgcg tactgaaatg ctggactggt ttaactcccg
    18601 aaccggcaat attctgcgtc agcaaatctc ctttgcggaa tccgacctac cactgatcta
    18661 ccgcacagag cgggcattgc agcttatctg cgacaagctc cagcaggtag agctgaagcg
    18721 cgttccgcgc gtggagaatc tactctattt tatgcagaac acgcgtaaac ggcttgaacc
    18781 ccagccgaag agtaacgcag agaacgccgc acagaccacg gtcagaacgc tgatttatgc
    18841 cccggaaaca atggcttcct ccacgccaga agcggtagtg cctcccctgc ccgacctgcc
    18901 tgagatgaaa gtggaagtgc gcagtctgtc agaaaatccc ccacaggcca gtgtgatgaa
    18961 gcaaggcagt acggtaagag ggtttatcgc agggatcgtc tgttcagtgg ctatcgcctc
    19021 agcattgtgg tggtggcagg tctatccggc gcagcagcaa ctgttacagg ttaacgacac
    19081 cgcgcagggc gcagcaacgg tgtggatggc ctcacctgaa cttgaaaact atgagcgcag
    19141 gctgcatcag cttcttggcg tctccccggt acaaccgctg gaaaccggga tgcagatgat
    19201 gcgtgttgcc gacagtcgct ggccggaaag cctgcaacag cagcaggcct cgacacaatg
    19261 gaatgaggca ctcaaaaccc gcgcacagag tagcccacag ttgcgcggct ggttgcagac
    19321 ccgccaggac ttacatgctt ttgctgatct cgtgatgcag cgcgagaaag agggattaac
    19381 cctttcctat atcaaaaatg tcatctggca ggcggagcgg ggactggggc aggaaacacc
    19441 tctggaatcc ctgctgacgc agtaccagga tgcccgtgcg cagaagcaga atacagatgc
    19501 gctggaaaaa cagattaacg agcggatgga cggcgtgtta agtcgctggc tgctgctgaa
    19561 gaataatacg ataccgacga taaaaaaaga cattgaattt caataacatt catgaataca
    19621 aaggagtttt aaatggcgaa tttaatttat ttaacactaa atggtgataa tcaagggctc
    19681 atttcaagtg gttgttcatc acaaccatcc attggaaata aagctcaaac agcacacatt
    19741 gatcaaatta tgatttatga attcatgcat ggattaaatc gagatcaaaa cgtcaaccat
    19801 catcatttga caataaaaaa acctatagat aaagcatcac ccttattagg caaagcaatt
    19861 tgcgacaatg aattattgac atgtgacttt tccttttatc gtacaaataa atttggaatt
    19921 aatgaactgt actataaaat caaattaact ggagcaaaga taagcgacat tcatgtctct
    19981 atatcacata ttgttgttga taacagcatt caacccgaag agtcagtttc tttcagttat
    20041 gagtcaatta tatgggagca ttgttccgct ggcactagtg catacagttt atgggaagat
    20101 cgtctttttt aaaaata
//
LOCUS       DAZGHF010000084        19790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGHF010000084 DAZGHF010000000
VERSION     DAZGHF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.084"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..704)
                     /locus_tag="ACHK3A_003181"
     CDS             complement(<1..704)
                     /locus_tag="ACHK3A_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO8741231.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(841..1293)
                     /gene="moaE"
                     /locus_tag="ACHK3A_003182"
     CDS             complement(841..1293)
                     /gene="moaE"
                     /locus_tag="ACHK3A_003182"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO8741232.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1295..1540)
                     /gene="moaD"
                     /locus_tag="ACHK3A_003183"
     CDS             complement(1295..1540)
                     /gene="moaD"
                     /locus_tag="ACHK3A_003183"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO8741233.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSLDHPLTDGDEVAFFPPVTGG"
     gene            complement(1533..2018)
                     /gene="moaC"
                     /locus_tag="ACHK3A_003184"
     CDS             complement(1533..2018)
                     /gene="moaC"
                     /locus_tag="ACHK3A_003184"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO8741234.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2021..2533)
                     /gene="moaB"
                     /locus_tag="ACHK3A_003185"
     CDS             complement(2021..2533)
                     /gene="moaB"
                     /locus_tag="ACHK3A_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO8741235.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2555..3544)
                     /gene="moaA"
                     /locus_tag="ACHK3A_003186"
     CDS             complement(2555..3544)
                     /gene="moaA"
                     /locus_tag="ACHK3A_003186"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO8741236.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3532..3676)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3941..4849
                     /gene="yvcK"
                     /locus_tag="ACHK3A_003187"
     CDS             3941..4849
                     /gene="yvcK"
                     /locus_tag="ACHK3A_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO8741237.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5041..7062)
                     /gene="uvrB"
                     /locus_tag="ACHK3A_003188"
     CDS             complement(5041..7062)
                     /gene="uvrB"
                     /locus_tag="ACHK3A_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO8741238.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7641..8318)
                     /gene="bioD"
                     /locus_tag="ACHK3A_003189"
     CDS             complement(7641..8318)
                     /gene="bioD"
                     /locus_tag="ACHK3A_003189"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO8741239.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(8311..9066)
                     /gene="bioC"
                     /locus_tag="ACHK3A_003190"
     CDS             complement(8311..9066)
                     /gene="bioC"
                     /locus_tag="ACHK3A_003190"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO8741240.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSMALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDKIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9053..10207)
                     /gene="bioF"
                     /locus_tag="ACHK3A_003191"
     CDS             complement(9053..10207)
                     /gene="bioF"
                     /locus_tag="ACHK3A_003191"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO8741241.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSDTVADYLVQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDTRREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10204..11244)
                     /gene="bioB"
                     /locus_tag="ACHK3A_003192"
     CDS             complement(10204..11244)
                     /gene="bioB"
                     /locus_tag="ACHK3A_003192"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO8741242.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLASARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11331..12620
                     /gene="bioA"
                     /locus_tag="ACHK3A_003193"
     CDS             11331..12620
                     /gene="bioA"
                     /locus_tag="ACHK3A_003193"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO8741243.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHADIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEGQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTVAVNRAVQDETFFCQ"
     gene            12679..13155
                     /locus_tag="ACHK3A_003194"
     CDS             12679..13155
                     /locus_tag="ACHK3A_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO8741244.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNIHFHSLASASITAMFS"
     gene            13307..14590
                     /locus_tag="ACHK3A_003195"
     CDS             13307..14590
                     /locus_tag="ACHK3A_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO8741245.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(14731..16992)
                     /locus_tag="ACHK3A_003196"
     CDS             complement(14731..16992)
                     /locus_tag="ACHK3A_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO8741246.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(17175..18608)
                     /locus_tag="ACHK3A_003197"
     CDS             complement(17175..18608)
                     /locus_tag="ACHK3A_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO8741247.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(18684..19736)
                     /locus_tag="ACHK3A_003198"
     CDS             complement(18684..19736)
                     /locus_tag="ACHK3A_003198"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO8741248.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLASIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISCSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT         4719 a         5343 c         4980 g         4748 t
ORIGIN      
        1 taacggcggt tgccgaagat ccgcaacaac atcaggaaca agttgatgaa gtccagatac
       61 aaggttaacg cgccaagaat ggaatatttg cgcaggttcg acgtgtcgcg ggtatcaatc
      121 tgctcaccca tatttttcaa tttctgcgtg tcatacgccg tcaatccgac aaagacaatc
      181 acgccaatgt aggtaactgc ccacatcaat gcttcgcttt tcagccagaa gttgaccagc
      241 gatgccagca caatgccgat taacgccata aacagcatat tgccaaagcc gcttagatcg
      301 cgcttcgtgg tgtaaccgta caggctcatt gcgccaaaca ttccggcagt aacgacgaaa
      361 gtactggcga tagaagcagc ggtataaaca atgaaaatac tggaaagcgt aagacccgtc
      421 agcgccgaat aaagcataaa gagcatcgtc gttacacctg cgctcagctt ttgaatcatc
      481 gctgataaca caataaccaa tgctaattgc gcgatgatca gaccgattaa aaagacacgg
      541 ttagtgaaca acagctccat cacagccgcg gaattagccg cataccaggc aacaaatgcg
      601 gtcagcaaca agccaacggt catccagcca tagacttgcg ccatataagt ttgcaagcca
      661 gcccggggtt gtacaattga atcagaacgt gggaatctgt ccatgacgat ctcctgaaga
      721 tataaggaat atcttaagga tactgcaaaa tgatgaggct gtgcatcgac gcagcgtaaa
      781 cgcatgtact gagcggtgaa attgccggac gcagcggtgc cttatccggc taacaaaaaa
      841 ctaccagcgt tttgccgcct gctgatcgct ctcccgagct tcaacccagc ggtcgccttc
      901 cggcgtggct tcacgcttcc agaacggtgc gcgggttttg agataatcca taataaactg
      961 cccggcttca aacgcactgc tgcgatgcgc actggtgaca ccgacaaaaa cgatttcatc
     1021 tcccggccat aattccccga tgcggtgaat caccgtgacg cgccccagcg gccagcggtt
     1081 acgcgcttca tcgacaattt ctgccagtgc tttttcagtc atccccggat agtgttcgag
     1141 ggttaatgct ttgacgctgt cgccaaggtt atggttgcgc accttaccgg taaaggtgac
     1201 taccgcaccg tcttcatcac gctccgccag ccacggatac tcttctccta cgctgaatgg
     1261 ctgcgggccg acaacaattt tggtttctgc catcttaacc tccggttacc ggcgggaaga
     1321 aagccacttc gtcgccgtca gtcagcggat ggtcaagact caccagcgtc tggttgacgg
     1381 cagccagcaa tttgccatct tccagcgcca gcgcccagcg atcgctctgc gcagccagat
     1441 gttggcgtaa cgcttcaacg gttgggaaat ccgcagccac ttcggttgca tctgttccca
     1501 ccaactcgcg cacctgggcg aaaaaaagaa ctttaatcat ccgcttccac cttaaagtca
     1561 cccgacttgc cgccgctctt cgccagcaaa cgtaccggac caattaccat atctttttgc
     1621 accgctttgc acatgtcata aatggtcagc gccgccacgg aggccgcggt taacgcttcc
     1681 atttcgacac cggttttccc ggtcaggcgg cataaggttt ctatacgtac ccgattgtgc
     1741 tccggctcgg cctgtaaatt gacttcaact ttgctgagca tcagcgggtg acacagcggg
     1801 atcagatccc aggtgcgttt tgccgcctga ataccggcga tacgcgcagt ggcaaatacg
     1861 tcgcctttgt ggtggcgacc atcaataatc atcgccagcg tctcgctgcg catggtgaca
     1921 aaggcttccg cccgcgcttc acgcacggtt tccgctttgg cggagacatc caccatgtgt
     1981 gcttcgccag cggcgttgat atgggtcagt tgcgacatac ttatttcttc aaatgtggat
     2041 ggaaattgca tggacgtgta cgggcatcga gctgcggcgc gatgatattt tcccacgcgg
     2101 tgcggcacgc tttggtcgaa cccggcatgg cgaaaatcag cgttttgtta gcgacgcccg
     2161 ctaccgcacg agattgcaac gttgaggtgc caatctcttc aaacgacaac atccggaaca
     2221 cttcaccaaa accttcaact tcacggtcga acaacggcag cagcgcttcg ggagcctgat
     2281 caccttccgt caggccagta ccacccgtaa tcaataccac ttgtacatcg tcgctggcga
     2341 tccacgcaga tacctgagcg cgaatagcgt agcggttttc tttcacaatg gctttatcga
     2401 caacgtgatg gcccgcttcc tgcgccgaat cgcgcagata gtgaccggag gtatcgtctt
     2461 cttcaccacg acgattagaa accgtaagaa tagcaatacg ggtcgggata aattcagtgc
     2521 ttacctgact catctgatct ctccttttga cgttttagcc gccaatgtac gataagtttt
     2581 gcgtaatacc ggtgttgttt tgatgcagga aatgggtctg tttcttctcc cgcagcgccg
     2641 ctgaaatacg cgcttccagc gcctgttgct gggtatcgtc ttccagcaga tcgcgcaggt
     2701 taacgccgcc ttcaccaaac aggcagagat ggagtttacc aatggaggaa acgcgcaggc
     2761 ggttgcaagt ggcgcagaag tctttttcat acggcatgat aaggccaatc tctccggcgt
     2821 aatccggatg acaaaagact tgcgcgggac cgtcgctgcg ttgacgtaat tggtggatcc
     2881 agccgcgacg cagtagctcg tcacgcagaa cctgaccaga gatgtgatgc ttacggaaga
     2941 gctcgctgcc ctcacccgtt tccatcaatt cgatgaaacg cagctggata gggcgatgct
     3001 ggatccagtt cagaaaggtg tcgagctgat gatgattaac atcacgcatc agcacggtat
     3061 tgactttgac cttctcaaaa ccggcctcaa atgcagcatc gatccctgcc atgacctggt
     3121 tgaatttatc ctgcccggta atggcatgaa actggcgagc gtcaaggcta tcaacgctga
     3181 cgttgatgcc agtaagtccc gcatcgcgcc agttcgccac atcgcgttcc agacggtaac
     3241 cattggtggt gaccgcaatc tggcggatag cgtcgttttc ccgcacagcg gcgatgatat
     3301 cggtaaagtc gcggcgtaaa gacggctcac cgcctgtcag gcgcactttt tcggtgccca
     3361 gactggcaaa ggcgcgcgta acccggcgaa tttcatcgac ggtaagaaag cctttattgg
     3421 tgacgccgct cggtttgtag ccatccggca ggcagtaggt gcaacgaaag ttacacacat
     3481 cagtaatcga caggcgcaag tagtaaaact tacgcgcaaa tgcatcagtc agttgtgaag
     3541 ccatgtacac ctttccagat acgggaggcg aagtcatttc ttccttcgcc ctggtggcaa
     3601 tctttacgag cattgtcacg gccaaagcac cgtatcagtt gacccaggtg cagaggctag
     3661 agtgtttagt ggttaagccg atactagcgt gtaaatgaga tttttaccat ccacactttc
     3721 gctatgtaat catgtatata gcgtcatgat cgtgctattt cgctacagaa taagggaaaa
     3781 tggcggtttt actttgatat acatcatcag gaaggcatag cgtacatcgt cctttagagg
     3841 tagaagaaaa tagtttgtat cctcaatatt ggcaggttaa ttgctgtttc cccgcaattt
     3901 gcgctactgt agcgcggata atctgactcc aggaaactat atgcgcaatc gtacgctggc
     3961 tgaccttgat cgtgtcgttg ctctcggcgg agggcatgga ctgggacgcg ttctctcatc
     4021 actttcgtct ttgggttctc gtttaacggg tatcgtcacc accacggata atggcggatc
     4081 gacagggcgt attcgccgct cagaaggcgg tattgcctgg ggcgatatgc gtaactgcct
     4141 caaccagctg ataactgaac ccagcgtcgc ttccgcaatg tttgaatatc gtttcggcgg
     4201 taatggcgaa ctttccgggc acaaccttgg aaatctgatg ctgaaggcac tggatcacct
     4261 tagcgtgcga cctcttgaag caatcaatct gattcgcaat ctgctgaaag tggacgcaca
     4321 tttgattccg atgtcggagc atccggtgga tctgatggcg attgacgatc aggggcatga
     4381 agtttacggc gaggtcaata tcgaccagtt aactacgccg attcaggagt tactgttaac
     4441 gccgaatgta cccgcaacgc gtgaggctgt tcacgctatc agtgaagcgg atctcatcat
     4501 tattgggcct ggcagttttt acaccagcct gatgccaatt ctgctgctga aggaaatcgc
     4561 acaggcatta cgccgcacgc cagcgccgat ggtctatata ggcaatctgg ggcgtgagtt
     4621 gagtttacct gcggctaatt tgaagctgga aagcaagctg gcaattatgg agcagtatgt
     4681 cggtaaaaag gtcattgatg cggtcatcgt cgggccaaaa gtggatgtct cggcggtgaa
     4741 agagcggatt gtgattcagg aggtactgga ggccagcgat attccgtatc gtcatgaccg
     4801 ccagttgtta catagcgcgc tggaaaaggc attacaggct ttaggttaac ggaaagccga
     4861 aggaaaattc cggcttccta ttgaagacaa agtgcgcgtt gtttatgccg gatgcggcgt
     4921 gaaagcctta tccggcctac aaaccgcgct aattcaatgt attgcagaga tcatgtaggc
     4981 ctgataagcg tagcacatca ggcaattttg cttttgtcat ctgtcttctt cgttatcctg
     5041 tcacgaagcc gcaatgaaca actcacgcag ctgatgcaac tggtcacgaa tttgtgccgc
     5101 ttcttcgaac tccagattct gcgcgtgttg catcatcaac ccttccagtt catggatttt
     5161 ctgctgcaac gctttaggcg acatatccat cggcacatta tccggctcaa caatcggtcg
     5221 cgattttcct ctgcccttcg ctttggtttt ggcaatgttc tgccccagcg ccaggatatc
     5281 gaccactttc ttgttcaagc cttgcggcgt aatgccgtgt tcctcgttgt acttctgctg
     5341 tttctcgcgg cgacgttcgg tttcgccaat cgctttcgcc attgatgggg tgatcttatc
     5401 gccgtagaga atcgctttac cgttaacgtt acgtgccgca cgaccaatgg tctggatcaa
     5461 cgaacgttcg gaacgcagga agccttcttt gtcagcgtcg aggatcgcca ccagcgaaac
     5521 ttccggcata tccagacctt cgcgcagtaa gttgatccct accaatacgt cgaactcacc
     5581 cagacgcaag tcgcggataa tctccatacg ttcgacggtg tcgatatctg agtgaagata
     5641 acgcacgcgc tcaccgtgtt cttcgagata ttcagtgaga tcttccgcca tccgcttggt
     5701 cagagttgta accagtacgc gttcgttaat tgccgctcgc tgacgaatct ccgaaagaag
     5761 atcatccacc tgtgttgcca ctggccgcac ttcgataatc gggtcgagta agcctgttgg
     5821 acgcaccacc tgatccacca catcgccacc ggatttttcc agctcgtaat tacccggcgt
     5881 cgccgaaaca tagatggttt gcggcgctaa tgcttcgaac tcttcaaatt tcagcggacg
     5941 gttatccagc gctgatggca ggcggaagcc gtactccacc agtgtctctt tacgcgcccg
     6001 gtcaccgcga tacatgccgc caatttgtgg aatggtgacg tgagattcat cgaccaccag
     6061 cagcccatca gcaggcaggt aatcaaacag cgtcggcggt ggctcacccg gtccacgacc
     6121 ggagaggaag cgcgagtagt tctcaatccc cgaacagtag cctagctcgt tcatcatctc
     6181 cagatcaaac tgggtacgct gggtcagccg ctgctcttcc agcagtttgt tgttttccaa
     6241 tagcacttta cgtctggcgg ccagctcttc tttgatctcc tccatcgcct ggacgatgcg
     6301 ctcgcgtggc gtgacgtagt gcgttttcgg gtagatggta aaacgtggaa tagtggaaac
     6361 aatctgcccg gtcagcgggt caaataacga caatcgttcc acttcctcgt caaacagctc
     6421 cacgcgaagt gcaatgtcat ccgattctgc cgggaagata tcaatcacct cgccacgaac
     6481 acggaaagta ccacgctgga aggcttgatc attacgagca tattgcagct ccgccagtcg
     6541 gcgcaggatc gcgcgctgat cgataatcat accgaccgtg agatggagca tcatcttgag
     6601 atataaatca ggatcgccca gaccataaat cgcggaaaca gacgccacca caaccacatc
     6661 acgccgctcc agcatcgctt tggtggcgga caaacgcatc tgttcgatat gttcgtttac
     6721 cgaggcatct ttctcaatga aggtgtcgga actcggtaca taggcctccg gctgatagta
     6781 gtcgtagtag gagacgaaat attccaccgc gttttccggg aagaattctt tcatttcgcc
     6841 atacagctgg gccgccagcg ttttgttggg cgcaagtacc atggttgggc gctgaaggtc
     6901 agcaatgaca ttggcaatgg tgaaggtttt cccggagcca gtcacgccaa gtaacgtctg
     6961 gtgcgccagg ccatcttcca gcccctcttc gagacgtcga atcgcctccg gctgatcgcc
     7021 agaaggttta aaagcggaat tcagtttgaa cggtttactc atgagtcgct acctgaagga
     7081 gttgggcggg caggtatgta attttactcg tcgtacttaa ttatgccaac aaattatact
     7141 ggataaaaaa acagttcatc accataatat ttctgttaca gcgtaaactc cgctgtcaat
     7201 catgagcaaa atttactctg cggcgagata aaactccggc cttaccgggt tatccccaaa
     7261 gcaacggctt ttttaacatt tgtcaagatg attgatgaca gttttatggc aagagatgcc
     7321 tgttcagtga ctgccattgc aattatctaa cctgttaaaa aataaaagat atttctttga
     7381 gccgtcttta acgccaattc gcgtgcaagc cgcgtattct ctcgcttgcc tcgtgttttc
     7441 taactctaat acacatggtt atccacagga atagtggata actgtctgca gcccctatcc
     7501 cccgccgcct gggcaattcc cacattcacc gcatgatgcg gaggtaaaat ttttttcgtg
     7561 atttttttag aatttaattg gttaaattgc agtcaatcgg agacacgatc tcgctcgcaa
     7621 tttaaccaat tacaggatga ctacattaag gcaagattga tgtactttcc ggttgccgca
     7681 ttttctggat tttctgcaag ccagggaatt tcgcccagca gcggcgcggg aatcatgcgg
     7741 gtgagcgtgg tcatatattc agcgtgacgt tttcccggag gcgtgacatc gttcgccacc
     7801 caccccgcca gagtcagtcc ggcgtgtagt attgcctgcg cagtcaacat cgcgtgatta
     7861 atacagccga gtttcacacc aactaccagt atcaccggca gttgttcctg tgttacccaa
     7921 tccgcaaaag tgaaagtgtc agaaagcggc gtaaaccagc cgccagcacc ttccactaac
     7981 acccagtcag cctgttgttc aagcgcgcgt aatccggcgc tcattaccaa tgattctatc
     8041 ggcctgccct cttgcgcgct gatgatatgc ggcgaggtgg gttctgcgaa ggtgtaagga
     8101 tttaccgttg cgtaatccag ctgcaggcta ctgttgcgct gtaacgccag cgcgtcacta
     8161 ttacgtaagc ctttcggggt cttttcgctg ccagaggcca ccggtttata acctgccgtc
     8221 cggtagcctg ctgcctttgc ggcttgtaaa agcgcgcagc tggcgacagt tttccccact
     8281 tcggtatccg ttccggtgac aaaataacat ttactcacga gcaatcactc ccaaaaaaag
     8341 atgatacgtc agaggatatc gcccctgttg ttgcggccag gctagttgca atcgctgcaa
     8401 ctgcgaacgc gttaatattc gcgggtcgcg cccttcatga agatgcgtgg caccgatgcc
     8461 tttcagcgaa cgcatggcac tgagcgcatc atcaaaccac agcgtaatgg gctgaatata
     8521 atgttgataa tgcacggcgt ttagcgactg ttcgatttta tctggcggta aaaagcgatt
     8581 agcatgcggg cgctcgtcca ccgcctgcca cgcctgatgc agttcgggta acgatccctg
     8641 caccagcgtg gtaaacgcga ccacgcctcc ggggcgcacc acccgataca gctcgcggag
     8701 tgccatggat aaattaccgc accactgcac cgcgagattg ctccatgcaa gatcgaacgt
     8761 tgcagtcgct aacggcaggg attcgatatc tcccgccaga taatggtctg cggcatcctt
     8821 ctggcgtgcc tgaacaagca ttggcggaga gagatctaac gcagtcacct gcgcgtgacg
     8881 ctctcgccag cggcggctca tccagcctgg tccacaaccc gcatccagta cgtgggtgta
     8941 tttacgctgt gggagcattg ccagtaaggc gtcagcactc tggcgctgta gatcggcgtg
     9001 ttgctcatag tgtgcggctg cccgtccaaa tgccgctgca atggcttgtt tattaaccgt
     9061 tgccatgcag cacctccagc agacggtcga tatcctgcat ttcatgcgcg gctgttagtg
     9121 ttaagcgcag tcgcgcagta ccagcaggta cggttggcgg gcgaatcgcc gtgacccagc
     9181 agccttgctg gcgcagtttt tctgccagtt gtaacgcacg gctgttatca ccaacaatca
     9241 atggctggat ggcgctgcat gaatcagcaa gcgtaaacgg caaatcctgt actccggcac
     9301 gaaaacgcgt aatgagtgcc gccagttttt cgcgccgtgt atcaccctca tcactgcgaa
     9361 tgaccgccag cgatgcacgt aatgcctgcg cctgagcggg cggcatactg gtgctgtaaa
     9421 taagatggcg ggcaaattgc accagataat ccgccaccgt atcggagcaa agcaccgctg
     9481 ccccgctgac gccaaatcct ttgccaaaag tcaccaccag caattctggt tttacctttt
     9541 gcagccagca gctgccgcgc ccctgctccc cgataacgcc cgtgccgtgg gcgtcatcga
     9601 ccatcaacca gccatcgtgc tgttgcgtta cctgctggat ttcctccagt ggcgcactat
     9661 cgccgtccat gctgaacaca ccttctgtca ctaccagttg ctgccccgga cagggggaag
     9721 caagcagtcg cgccagatga gtgacatcgt tatgagcaaa acggcgaagc ggcgacgggc
     9781 ttaaacttgc agcctccagc aatgaggcat ggctaagccg gtcggcaaca atacggtcct
     9841 ctttcgccat catcgcggca atgactgcct gattagccgc aaaaccggag ataaacagca
     9901 gcgcccgcga atagcccagc cactcggcca gttcttcttc cagcgcctga tgtgccacgc
     9961 tataaccgct gacgtgaccg gagccgccgc taccgacgcc aaattgctcc gcactctgct
    10021 tccaggcacg gataatttgc ggatgatggc ttaaacctaa ataatcgtta ctggaaaagt
    10081 tcagatactg gcgatcgtcc gccaccagcc agcgtccggc cccttgcgcc accggataac
    10141 ggcgacgcag ggcatcggca gcacgccgcg catcgagcgc cgcgttgatt ttctcctgcc
    10201 agctcatagt gctgccgcgt tgtaatattc gtcagtgtcc ggggtcatca gtgcctgctc
    10261 cagacgctgc tgttgttcgt tatcccctgc cagcacggca gtttgctgtg gatttagccc
    10321 cagtttgcgg aacagttgca ggtctttatc ttcttccgga ttcggcgtgg ttagcagttt
    10381 gcaaccgtag aaaatcgagt tagccccggc cataaagcac atcgcctgag tctgttcatt
    10441 catctgctcg cgtccggcag aaaggcgcac gtatgaggtc ggcatcatga tccgcgctac
    10501 ggcaatggtg cgaataaaat caaaggcatc gacatcatcg ttatctgcca gtggagtacc
    10561 tttcaccttc acgagcatgt tgattggcac gctttccggc ggcgtcggca ggtttgccag
    10621 ttgcagtaat aatccggcac gatcttttac cgtttcgcct aaacccacaa tgccacctga
    10681 gcagactttg atcccggcat cgcgcacttt ttccagcgta tcaaggcgtt cctgataagt
    10741 acgtgtggtg atgatattgc cgtaaaactc cggcgaggtg tcgaggttat ggttgtagta
    10801 atccagcccg gcattcgcaa ggcgctgcgc ctgagattca ctcaacgtac ccagcgtcat
    10861 acacgcctcc agccccatcg cttttactcc ctgcaccatt tgttccaggt agggcatatc
    10921 gcgttcgtgg ggattcttcc acgccgcgcc catgcagaag cgcgtcgatc ctgccgcttt
    10981 cgctttgcgc gccgacgcca gcacctgttc aacttccatc aaccgctcgg cttccagccc
    11041 ggttttgtag cgcgagcttt gcgggcagta tttgcaatct tccggacaag ctccggtctt
    11101 aattgacagc aaagtgctga cctgcacctg acgtggatcg aaatgctgac gatgcacctg
    11161 ctgcgcttca aacagcagat ccagcaacgg tttttcaaat aattctgtga cttgcgacaa
    11221 tgtccagcgt gggcggtgag ccatggggct tctccaaaac gtgttttttg ttgttaattc
    11281 ggtgtagact cgtaaaccta aatcttttca atttggttta caagtcgatt atgacaacgg
    11341 acgatcttgc ctttgaccaa cgccatatct ggcacccata cacatccatg acctccccgc
    11401 taccggttta tccggtggcg agcgccgaag gttgcgagct aattttgtct gacggcagac
    11461 gcctggttga cggtatgtcg tcctggtggg cggcgatcca cggctacaat cacccgcagc
    11521 ttaatgcggc gatgaagacg caaattgatg ccatgtcgca tgtgatgttt ggtggtatca
    11581 ctcatgcgcc agccattgag ctgtgccgca aactggtggc gatgacgccg caaccgctgg
    11641 aatgcgtttt tctcgcggac tccggttccg tagcggtgga agtggcgatg aaaatggcgt
    11701 tgcagtactg gcaggccaaa ggcgaagcgc gccagcgttt tctgaccttc cgcaatggtt
    11761 atcatggcga tacctttggc gcgatgtctg tgtgcgatcc ggacaactca atgcacagcc
    11821 tgtggaaagg ctatctgcca gaaaacttgt ttgctcccgc cccgcaaagc cgcatggatg
    11881 gtgaatggga tgaacgcgat atggtgggct ttgcccgcct gatggcggca catcgtcatg
    11941 aaatcgccgc cgtgatcatt gaaccgattg tccagggcgc aggcgggatg cgcatctacc
    12001 atccggaatg gttaaaacga atccgcaaaa tgtgcgatcg cgaaggtatc ttgctgattg
    12061 ctgacgagat cgccaccgga tttggtcgta ccggcaaact gtttgcctgt gaacatgcag
    12121 atatcgcgcc ggatattttg tgtctcggta aagccttaac cggcggcaca atgacccttt
    12181 ccgccacgct caccacgcgc gaggttgcgg aaaccatcag taacggcgaa gccggctgct
    12241 ttatgcatgg gccaactttt atgggcaatc cgctggcctg cgcagcagca aacgccagcc
    12301 tggcgattct cgaatctggc gactggcagc atcaggtggc ggctattgaa gggcagctac
    12361 gcgagcaact tgcccccgcc tgtgacgccg aaatggttgc cgatgtgcgc gtactggggg
    12421 caatcggcgt ggtcgaaacc actcgtccgg tgaatatggc agcgctgcaa aaattctttg
    12481 tcgaacaggg tgtctggatc cggccttttg gcaaactgat ttacctgatg ccgccctata
    12541 ttattctccc gcaacagttg cagcgtctga ccgtagcggt taaccgtgcg gtgcaggatg
    12601 aaacattttt ttgccaataa ccgggagtca gcgtgagggg ttctggctac actttctgca
    12661 aactagaaag gagggttcat gaaactcatc agtaatgatc tgcgcgatgg cgataagttg
    12721 ccacatcgtc atgtctttaa cggcatgggt tacgatggcg ataatatttc accgcatctg
    12781 gcgtgggatg atgttcctgc gggaacgaaa agttttgttg tcacctgcta cgacccggat
    12841 gcgccaaccg gctccggctg gtggcactgg gtagttgtta acttacccgc tgatacccgc
    12901 gtattaccgc aagggtttgg ctctggtctg gtagcaatgc cagacggcgt tttgcagacg
    12961 cgtaccgact ttggtaaaac cgggtacgat ggtgcagcgc cgccgaaagg cgaaacccat
    13021 cgctacattt ttaccgttca tgcgctggat gtagaacgta ttgatgtcga tgaaggtgcc
    13081 agcggcgcga tggtcgggtt taacatccat ttccactctc tggcaagcgc atcgattacc
    13141 gcgatgttca gttaattact ctgccagatg gcgcaaagcc atctggtatc acttaaaggt
    13201 attaaaaaca actttttgtc tttttacctt cccgtttcgc taaagttagt ataaaaaagc
    13261 aggcctcaac ggattcattt ttctatttca tagcccggag caacctgtga acacattttc
    13321 agtttcccgt ctggcgctgg cattggcttt tggcgtgacg ctgaccgcct gtagctcaac
    13381 accgcccgat caacgtcctt ctgatcaaac cgcgcctggt acctcttctc gcccgattct
    13441 gtcggcaaaa gaagcgcaga atttcgatgc tcaacactat tttgcatccc tgacaccagg
    13501 tgcggcagcg tggaatcctt ccccgattac cctgcctgca caacctgact ttgttgtcgg
    13561 cccggcgggt actcaaggtg taacgcatac cacgattcag gcggcggtag atgcggcaat
    13621 tatcaagcgt accaacaagc gccagtatat tgccgtgatg cctggtgagt atcagggaac
    13681 ggtatatgtc cctgccgctc cgggtggaat tactctgtac ggtacaggtg aaaaaccgat
    13741 tgatgtgaag attgggcttt cccttgatgg gggcatgagc cccgccgact ggcgtcacga
    13801 cgtcaacccg cgcggcaaat atatgccagg taaaccagcg tggtatatgt acgatagctg
    13861 ccagagcaaa cgcagcgaca gtatcggcgt tctctgctct gcggtcttct ggtcacaaaa
    13921 caatggactg caactgcaaa acctgaccat cgaaaacacg ctgggcgata gcgtagatgc
    13981 gggtaaccat ccggcggtgg cactgcgtac tgatggtgac caggtacaga ttaacaacgt
    14041 taacattctc ggtcgtcaga acaccttctt tgtcaccaac agcggtgtgc agaaccgtct
    14101 ggaaacgaat cgtcagccgc gtacgctggt gaccaacagc tacattgaag gggatgtgga
    14161 tatcgtttct ggtcgcggcg cagtggtgtt cgataatacc gaattccgcg tggtgaactc
    14221 acgtactcag caagaagcgt atgtgtttgc accggctacg ctgtccaaca tttactacgg
    14281 tttcctcgcc gtaaacagtc gtttcaatgc ttccggtgat ggcgtggcgc aactgggccg
    14341 ctcgctggat gttgatgcca ataccaacgg tcaggtggtg atccgtgata gcgccatcaa
    14401 cgaaggtttt aacacagcca aaccgtgggc tgatgcggtg atttccaatc gtccattcgc
    14461 gggtaacacc ggcagcgttg atgataacga cgaagtacag cgcaatctga atgacactaa
    14521 ctacaaccgc atgtgggaat acaataaccg cggcgtgggt agcaaagtgg ttgcagaggc
    14581 gaagaagtag gagctgttaa ctatgtgccg gatgcggcgt aaacgcctta tccggcctac
    14641 ggttcgatgc gatttgtagg tcggataaga tgcgccagca tcgcatccga caataagtgc
    14701 cggatgcgac ctacattcac atggcgcttt ttacatctga cggtttttat tgaagttaat
    14761 caaactaccc gccttgataa tctcgcgctc ttcagcagtc agactttcca tatagagcgt
    14821 aatctccgtt accggtgcat cttcatggat cacataacct ttaaacgtcg ttccaggatt
    14881 atccagcgcc gctttaatgc cagggatgta aatgtaatcc cccacttcaa acgttggtgc
    14941 ttccgccatt tgcagcggta acatccccca gttgatgacg ttagaacggt agcgtttagt
    15001 cgcgtattcc tcggcaatat tcgccagacc gccaatcaca cgctggcagc tcgccgcctg
    15061 ctcacgcgca gaaccatcgc ctggtttcac cgcatagacc atgctgccaa tttcagtttg
    15121 cagcggatca atatgctcct gaccagcaat ctgcttaatg cgcgcaaaca cctctgtcag
    15181 ctcgctgaca ttccccgcca gacgctgatt ttccagctca gcagtcgctt tacttctgcc
    15241 aacataaccg ggatcgcggc gtgacagggt aaactccgcc agaccaatcg gatttgaacg
    15301 ataagaagag gtttcaccgg aaggaatcag ttcgtcggtg gtggtgactt cgtcgaggat
    15361 cttcgagcac actttgagga cgatattgtc agtcagcgcg cccaattccg gccagtcttt
    15421 gatgttcggt ccgtagatca gcggttgctg ggtcgcccct ttcacaaagc cttgatagac
    15481 gcgattttta tacggcgtta catcgaaggc gtactccggc acgttgtccc agcaatcgag
    15541 ttcgctggca gaggttaaat agccaccgtt tgccgcagtt gcagcgatag aacgagcgtc
    15601 catcaacgcc accgctgaca tctgcccatt agctggctta gagccttcgc ggttcgggaa
    15661 gttacgcgtg gtgtggcgaa tactcaaacc gttgttgatt ggcgtatcgc ccgcaccaaa
    15721 gcatgggccg cagaacgcgg ttctgatgat tgcgcctgcg ccaatcaaat ctgctaccac
    15781 accttttttg gcgagatcca taaacaccgg ctgtgatgac gggtaaaccg ccaatgagaa
    15841 ggtatcgttg ccgcaggatt gaccgcgcag cgcattcgcc gccgcgatga cgttttcgta
    15901 gttaccgcca gaacagcccg cgataatccc ctgctgcact ttcaggcgac cattttccac
    15961 tttatccagc agcgagagtt tggctttacc gtgcgccacg cgttcggact caatttcaat
    16021 ctcacgcaga atgtcggtca ggttctggtt cagcgtgtcg atttcataca cgttgctcgg
    16081 gtggaatggc agcgcaatca ttggtttgat ggcgcttaaa tcaacgctga tgcagccatc
    16141 gtagtacgcc attggttgag ggttaagttg gcagtaatcc tggccgcgac cgtgcagcgc
    16201 cagccagtta tggacttctt catcggtttg ccagacagaa cttaaacagg tcgtttcagt
    16261 ggtcatcacg tcaacgctgt tacggaaatc ggtagagagc gctgcaacac cgggacctac
    16321 gaactccatc actttgtttt tgacgtaacc gtttttgaac acggcaccga tgatagccag
    16381 cgccacatcc tgcggcccca catacggcgc aggttttccg gtcagatgca ccgcaaccac
    16441 gcccggatag tcgatatccc aggtgtcatt aagcagctgt tttaccaact cgccgccgcc
    16501 ctcgccaacc gccattgtcc ctaacgcacc gtaacgggta tggctgtctg acccgaggat
    16561 cattttgccg ccgcccgcca tcatttcacg catatattga tggatgaccg caatatgcgg
    16621 tggcacaaaa ataccgccat aacgctgggc cgccgataaa ccaaaaacat ggtcatcacc
    16681 gttaatggtg ccgcccacag cacagagtga gttatggcag ttggtcagca catacggcag
    16741 cgggaaacgt tccataccgg acgctttagc ggtctgtaca ataccaacaa aggtaatatc
    16801 gtgagaggct aatgaatcaa acttaatttt aagtttatcc atatttccgg acgtattatg
    16861 agaagagaga atagaccagg caatagtgcc tttttttgct tcttcttttt ttatttcgcc
    16921 ggtgaaatgt tcttcggcaa ttatttcgtt attgctggcg agaaacacgc ctttttcaga
    16981 taacttgatc atcaaatgct ccagatatct gcgcaccgtg aagtgcgcag aaagataata
    17041 aatggatata ctctaaataa ttcgagttgc aggaaggcga caagcgagtg aatccccagg
    17101 agcttacata agtaagtgac tggggtgagc gaacgcagac gcagcacatg caacttgaag
    17161 tatgacgagt atatttacag catgttccag ccgatcagca tattccacca ccacacgccg
    17221 agggtgatat gcaccaggaa ggttaatatc gtcagtaccg caccgaccaa ccaccaggat
    17281 ttaatatcgt tgtaacccac gccaaagatg accggacctg ccgcgccgcc atagtgagta
    17341 accatgccgc cataggagtt agagaacaac agtgccagcg cggttaacat taacggtgcg
    17401 ccggagacgt tcgccagcat ggcaaataca ggtaacatag caacgatata ggcactaccg
    17461 gaagcgaaga aataacgcac gatgatgctg aggaaaataa taacgaagaa agcaacgtta
    17521 ccgtgaccat caaatgccag gttattttta aagacttcag ctaaccattc aaagaattta
    17581 actttcgata ataaggagct taagccgata ataccgccgt accagattaa ggtattccag
    17641 ccgcctttat ttttaaccac gtcttcccag gtaacgatac ccagcagcag catagtcgcc
    17701 ataacaacga ttgccacggt ggattcatca acccccagtg acttactgaa aatccagccc
    17761 agcagcgcca gcacgaagac accgagcagc attttttcgc ggattttcat cggtcccagt
    17821 tcggcaaggc ccgctttggc gatggttttg ttatccacct tcttaatttc tggcggatac
    17881 atggtgtaaa tcaccagcgg ggtgaccagc agcataatga tacccggcaa tccagcggcc
    17941 agcgcccatc caccccagct aatttgcagg tgcagaatgt cgttgatcat tttcagcgcc
    18001 agaatgttcc ccgccattgc ggtaaagaac atatagctgg tggttttagt gaccatgtaa
    18061 atggacatca tcaggtaatg tccaacacga cgcggacttt tttccggttc tgatcccaaa
    18121 gccactgcca cgctgttgat gatcggtaac acgatgccgc ccgcacgcgc ggtgttagac
    18181 ggtgttgccg gagccagtac cagatcgaga aataccgtaa cgtaacccag acccagggta
    18241 gtgctaccaa ttttaccaat cagcagatag gcaatacgtt tacctaagcc tgtggttaca
    18301 aacgcggcgc ttaaagtaaa cgcagaaaac accagccagg tggtaccgga agagtaaccg
    18361 cttaatacgg cggtggtttt aaattcccca cctgataagt tacccaccac taccatcgat
    18421 gcggcgacgg caattaacag tacgacaggt tccgggaaag gcttgataac cagccccaca
    18481 atggccgcca ggtaaatacc aaaaagcacc cacgccagtt cgcttaatcc tgccggagct
    18541 ggcataaagc caataataca tgggatcgct aatatcagaa ttagcttcca taacgatttt
    18601 ttattcattt aatatcctgt tctttctgta tgactcatac agaattcctt tattgattgt
    18661 cattatcacg acaacaaaat ttttcaggga agataaactt caccggaaaa tatttttctt
    18721 gtcgtccgaa taacagatgc gcgtaacgtc gtggcatcct gaccttcatt acttaaatga
    18781 acgtcgagcg caccactggg gtgttcaata ttgatattgc cgtatcctac agaagggacg
    18841 atttgtcggg tgacggtgcc ttccaatgca caactacagg aaatagcaat agcaccggtt
    18901 atcgccaaag cgcgatggca agaatgcggc ataaaataac gcacattaat tgccccgcct
    18961 ttctgcgctg gagaaataag tacaggttta gggataacca tattactgac atcgcctaag
    19021 cccattgctt tacccgcttg tagacggata gattcaatgc tggctaataa tgctttgtcg
    19081 gcatccagct ctgccggtaa ttcatagcct gtttttccca gatattcagc aggaataatg
    19141 acaactggca tcgccatatc gatacaggtc accgggacat cgtcaaaata atcaatctga
    19201 ttatccgtcg ggaagacttt tccggttttc gttccggcgg cattcaggaa agtgagcgca
    19261 accggtgcgg cagtacccgg tacgccgtca attctggcgc taccctcgta ctcgacaaca
    19321 ccatttggcg tttgcacatc agcttcgata aacgtacccg tattgacgtt gcggatacgt
    19381 acgcgggtaa ctggcgaagt cgctgcaatc aaaccatttt caatggcgaa tgccccaacg
    19441 ccagacagca tattgccgca gttaggcgtg gtatcgacac gttgctcatg gacgataact
    19501 tgagcaaaca gataatcgac atctgcacgc ggatcgctgg agcggctaat aatggcgact
    19561 ttactggtca gcggattacc gccgccaata ccgtcaattt ccagatcgtt accggaaccc
    19621 ataattgcca tcaatatttt atcgcgctgc gtttgatctt cgggtagatg ttccgctaac
    19681 aggaacgcgc ccctcgaggt tccacctcgc atcatcacgc agggtatttt tttcataata
    19741 atgccctcaa tttaaaaaca taacttattg cgatatggtt acattaaggg
//
LOCUS       DAZGHF010000085        19760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGHF010000085 DAZGHF010000000
VERSION     DAZGHF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.085"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            136..678
                     /gene="yfaZ"
                     /locus_tag="ACHK3A_003199"
     CDS             136..678
                     /gene="yfaZ"
                     /locus_tag="ACHK3A_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO8741249.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            778..1980
                     /gene="yfaY"
                     /locus_tag="ACHK3A_003200"
     CDS             778..1980
                     /gene="yfaY"
                     /locus_tag="ACHK3A_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO8741250.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLTRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEKQAMEKLWLDVKRVAGQSVIFEGTEGLPSQISRELQTRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2200..2982
                     /gene="yfaX"
                     /locus_tag="ACHK3A_003201"
     CDS             2200..2982
                     /gene="yfaX"
                     /locus_tag="ACHK3A_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO8741251.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     IWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2997..4202
                     /gene="rhmD"
                     /locus_tag="ACHK3A_003202"
     CDS             2997..4202
                     /gene="rhmD"
                     /locus_tag="ACHK3A_003202"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO8741252.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VISFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYNH"
     gene            4259..5548
                     /gene="yfaV"
                     /locus_tag="ACHK3A_003203"
     CDS             4259..5548
                     /gene="yfaV"
                     /locus_tag="ACHK3A_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8741253.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKEEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5565..6368
                     /gene="yfaU"
                     /locus_tag="ACHK3A_003204"
     CDS             5565..6368
                     /gene="yfaU"
                     /locus_tag="ACHK3A_003204"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO8741254.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAHVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAADKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGNY"
     gene            complement(6409..6642)
                     /locus_tag="ACHK3A_003205"
                     /pseudo
     CDS             complement(6409..6642)
                     /locus_tag="ACHK3A_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6643..7638)
                     /gene="rpnE"
                     /locus_tag="ACHK3A_003206"
     CDS             complement(6643..7638)
                     /gene="rpnE"
                     /locus_tag="ACHK3A_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO8741255.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDSEGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKIEGWQ
                     EGKLEGLQEGKLDGLQEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCH"
     gene            complement(7831..9021)
                     /gene="glpC"
                     /locus_tag="ACHK3A_003207"
     CDS             complement(7831..9021)
                     /gene="glpC"
                     /locus_tag="ACHK3A_003207"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO8741256.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9018..10277)
                     /gene="glpB"
                     /locus_tag="ACHK3A_003208"
     CDS             complement(9018..10277)
                     /gene="glpB"
                     /locus_tag="ACHK3A_003208"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO8741257.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLEALRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWSLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQL"
     gene            complement(10267..11895)
                     /gene="glpA"
                     /locus_tag="ACHK3A_003209"
     CDS             complement(10267..11895)
                     /gene="glpA"
                     /locus_tag="ACHK3A_003209"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO8741258.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQRIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTMSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12168..13526
                     /gene="glpT"
                     /locus_tag="ACHK3A_003210"
     CDS             12168..13526
                     /gene="glpT"
                     /locus_tag="ACHK3A_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO8741259.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            13531..14607
                     /gene="glpQ"
                     /locus_tag="ACHK3A_003211"
     CDS             13531..14607
                     /gene="glpQ"
                     /locus_tag="ACHK3A_003211"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO8741260.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYSGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            14810..15460
                     /gene="inaA"
                     /locus_tag="ACHK3A_003212"
     CDS             14810..15460
                     /gene="inaA"
                     /locus_tag="ACHK3A_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO8741261.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKSEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15514..15768)
                     /gene="yfaE"
                     /locus_tag="ACHK3A_003213"
     CDS             complement(15514..15768)
                     /gene="yfaE"
                     /locus_tag="ACHK3A_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO8741262.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(15768..16898)
                     /gene="nrdB"
                     /locus_tag="ACHK3A_003214"
     CDS             complement(15768..16898)
                     /gene="nrdB"
                     /locus_tag="ACHK3A_003214"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO8741263.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(16964..19249)
                     /gene="nrdA"
                     /locus_tag="ACHK3A_003215"
     CDS             complement(16964..19249)
                     /gene="nrdA"
                     /locus_tag="ACHK3A_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO8741264.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            19377..19520
                     /gene="ypaB"
                     /locus_tag="ACHK3A_003216"
     CDS             19377..19520
                     /gene="ypaB"
                     /locus_tag="ACHK3A_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO8741265.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     VIS"
BASE COUNT         4551 a         4885 c         5375 g         4949 t
ORIGIN      
        1 cgtttaatta tttaagaagt atttagttac aataagtgac tagatccctt ttttcgctca
       61 ttgaatcctc tgcgccaggc agtacaatcc cgcagctttg ggaattttga ttaattgctt
      121 gtgggaaagg ggataatgaa aaaaattgcg ctagcaggtc tggccggaat gctgttggtt
      181 tctgcatcgg tcaatgcaat gagcatcagc ggccaggcgg gtaaagaata caccaatatt
      241 ggtgtcggtt ttgggactga atcgacgggc ctggcgttaa gcggtaactg gacacataac
      301 gacgacgacg gtgacgtcgc gggcgtgggg ctggggttga atctgcctct cgggccgtta
      361 atggcgaccg ttggcggaaa aggcgtttac accaacccga attacggcga tgaaggttat
      421 gccgcagcgg taggaggtgg tttgcagtgg aaaattggca acagcttccg cttgtttggc
      481 gagtattact actctccgga ttcgctctcc agcggtattc aaagttatga ggaagcgaat
      541 gctggcgcgc gttacaccat tatgcgtcca gtcagtattg aggcgggtta tcgctacctg
      601 aacctttctg gtaaagacgg taaccgcgac aacgcagtgg ctgacggccc gtatgtgggg
      661 gttaacgcca gtttttgatc ccttccggcg cggcattctg ccgcgccatt cagtttctta
      721 ctacgcctta tctccctttg cgttatagtg attcgataac aaaagcagga gataaccatg
      781 ttaaaagtgg aaatgttatc caccggggat gaagtgttac acgggcaaat cgttgacact
      841 aacgctgcct ggctggccga ttttttcttt catcaggggt tgcctttaac tcgccgcaat
      901 acggtggggg ataaccttga tgacttagtg accattcttc gcgaacggag tcagcacgcc
      961 gatgtgctga tcgttaacgg cgggctgggg ccgaccagtg atgatttaag cgcactcgcc
     1021 gctgcgacag caaaaggtga aggcctggtg ctgcatgagg cctggctcaa agagatggaa
     1081 cgctatttcc acgaacgtgg acgagtaatg gcaccgagca accgtaaaca agcggagctg
     1141 cctgccagtg ctgaatttat caataacccg gtaggcaccg cctgtggttt tgccgtgcag
     1201 cttaatcgtt gcctgatgtt ctttaccccc ggcgtaccgt cagaatttaa agtgatggtc
     1261 gagcacgaaa tcctgccgcg cctgcgcgag cgtttttctt taccgcagcc gccggtttgt
     1321 ctgcgtttga ctacatttgg tcgttcggaa agcgatctgg cacaaagcct ggacaccctg
     1381 caactgccgc cgggcgtaac aatgggctat cgctcttcta tgccgattat cgaactgaaa
     1441 ctcaccggac cggcaagcga gaaacaggcg atggaaaaac tgtggctgga cgtcaaacgt
     1501 gttgccggac agagcgtgat tttcgaaggt accgaaggac tgccctcgca gatcagccgc
     1561 gaattgcaaa cccgccagtt cagcctgacg ttgagcgagc aattcacagg tggtttattg
     1621 gctttgcaac tttctcgcgc aggtgctcca ttgctggcgt gtgaagtggt tccttcacag
     1681 gaggaaaccc tggcgcaaac tgcgcactgg attacagaac ggcgggccaa ccattttgcc
     1741 gggctggcac tggctgtttc gggtttcgag aacgagcatc tcaactttgc gctagccacg
     1801 ccagatggca ctttcgctct gcgtgtgcgt ttcagcacta cacgctacag cctggctatc
     1861 cgtcaggaag tgtgcgcaat gatggcactg aatatgctgc gccgttggtt aaacggccag
     1921 gacatcgcca gtgagcatgg ctggattgag gttgttgagt ccatgacctt atctgtctga
     1981 ttccgccact ctgtgccggt ttactgcacc ggcacattcc ccctccggca aaatttgtcc
     2041 aacctgttgt actcaataag aaaaaatccg tgcgctaatt cacaaatttg tgcgtaattt
     2101 ctccagtccg ctggacgagg agcgggattt gatgagcttc acaattttac tccacctgag
     2161 caacgagact gattccagta tctaagactg gagtccagta tgttggaatc aagcaaagtt
     2221 cccgccctga cacgggctat tgatattctc aatttgattg cccgcattgg cccctgtagt
     2281 gctgcgacga tcattgacac attggggatc cctaaaagca cggcctattt gctgcttaat
     2341 gaactcaggc gtcagcgttt tcttagtctt gatcaccagg aaaatttttg tttgtggacc
     2401 aggctggtgg agctttccgg tcatgcgtta agcaagatgg atctccggga actggctcga
     2461 ccgcgtttga cgcaactgat ggataccacc gggctgttat gtcacctggg gattattgat
     2521 aacggcagcg cttattacat tttgaaagtg gagtcatcag ccactatcag cgtgcgttcc
     2581 catgaaggaa aaagcctttc gctttatcgc tccggaatag gtaaatgcct gcttgcctgg
     2641 caacctgcag ctgtacagca aagtattatc gaagggctga tctgggagca ggcgacaccc
     2701 acaacgatta ctcatccaca gcaacttcac gaagaactgg cgcgtattcg ccgtcagggc
     2761 tggagctatg acaacggcga agattacgcc gatgtccgtt gtgtcgctgc gccggtgttt
     2821 aacgccaata acgagcttac cgctgcgatc tcggtggtcg gtacccgctt acaaatcaat
     2881 gaagaatacc gtgattatct ggcgggcaag gccattgcct gcgccaggga tatttcacgt
     2941 ctgttgggat ggaaaagtcc ctttgactta caggcctcat aacaatggag aacagcatga
     3001 ccctaccaaa aatcaaacag gttcgcgcct ggtttactgg cggtgcgaca gcagaaaaag
     3061 gcgctggcgg cggtgattat cacgaccagg gggcaaatca ctggattgat gatcatattg
     3121 ccaccccgat gagtaaatac cgcgattacg agcagtcacg ccagtcattt ggcattaacg
     3181 ttcttggcac gttgattgtt gaagtcgaag cagaaaacgg ccagaccgga ttcgccgttt
     3241 cgacagccgg tgaaatgggc tgttttattg tcgaaaaaca ccttaaccgt ttcattgagg
     3301 gtaaatgtgt cagtgatatc aaactgatcc acgatcaaat gctcaatgcc accctgtatt
     3361 actccggctc tggtggcctg gtgatgaaca cgatttcctg tgtcgatctg gctctgtggg
     3421 atctgttcgg caaagtggtc gggcttccgg tttataaact tttaggcggc gcagttcgtg
     3481 atgagattca gttctacgcc acaggtgcgc gtccggatct ggcaaaagag atgggcttta
     3541 tcggtggcaa aatgccgacg cactgggggc cacatgatgg cgatgcgggg atccgcaaag
     3601 atgccgctat ggtcgcggat atgcgtgaaa aatgcggtga ggatttctgg ttaatgctcg
     3661 actgctggat gagtcaggac gtgaactatg ccaccaaact ggcccacgct tgcgcgccct
     3721 ataacctgaa atggatcgaa gagtgcctgc cgccacagca gtatgaaggt tatcgcgaac
     3781 tgaaacgcaa cgcgccagtc ggaatgatgg tcaccagcgg tgagcatcac ggcacactgc
     3841 aatctttccg tacgctttca gaaaccggta tcgacattat gcagccggat gttggctggt
     3901 gcggtggctt aaccacgctg gtggaaattg ccgcaatcgc caaatccaga gggcaactgg
     3961 tggtgccgca cggttcgtct gtttactctc accatgcggt gatcagcttc accaatacgc
     4021 cattcagcga attcctgatg accagcccgg attgttcaac gatgcgtccg caatttgatc
     4081 cgattctgct taatgagcca gtgccggtga atggtcgtat tcataaatca gtgcttgata
     4141 aacccggttt cggcgtcgaa ctcaatcgtg actgcaattt gaaacgcccc tacaaccact
     4201 aatcactccg ggcgttgctg tgacaacggt aacgcccaca tattctaagg agctttttat
     4261 gagcaccgct ttgcttgacg ccgtggtgaa gaaaaaccgt gcgcgtttaa ttccgtttat
     4321 gttggcgctg tatgtgctgg cgtttctcga ccgttcgaat atcggttttg ccaaacagac
     4381 ctaccagatt gataccgggc tgagtaatga agcttatgcg ctgggagcag gcattttctt
     4441 tgtggtatat gcgtttctag gtgttccggc gaatcttttg atgcgcaaac tgggggccag
     4501 aacctggatt ggtacgacaa cactgctgtg gggatttctt tcggcagcca tggcatgggc
     4561 cgatactgaa gcgaaatttc tgatcgttcg cactctgctt ggtgctgcgg aggccgggtt
     4621 cttccctggt atgatttatc tcacctcgca atggtttccg cagcgtaatc gcgccagcat
     4681 tatggggctg ttctatatgg gcgcaccgtt ggcgttaaca ctgggatcac cgctttccgg
     4741 cgcgctgctg gagatgcatg gatttatggg acatcccggc tggttctgga tgtttgtgat
     4801 tgaaggattg ctggcagtcg gcgctggggt attcacattc ttttggcttg atgacacacc
     4861 ggagcaggca cgttttctga gtaaagaaga aaaaacattg cttatcaatc aactggcaag
     4921 tgaagaacaa cagaaagtga cttctcgact gagcgatgcg ctgcgtaatg ggcgagtctg
     4981 gcaactggcg attatctacc tgaccattca ggtggcggtt tacggattaa ttttcttcct
     5041 gccgacccag gttgctgcat tgctggggac aaaagtgggc tttacggcgt cggtggtcac
     5101 cgccattccg tgggttgcgg ccttgtttgg gacctggctt attccgcgct attccgataa
     5161 aaccggcgaa cggcgtaatg tcgcagcgct gacattactg gcggcaggca ttggtattgg
     5221 tctctccggg ctgctttctc cagtactggc gatcgtagcg ctgtgtgttg cagccatcgg
     5281 gtttattgcc gtgcagccgg tgttctggac gatgccgaca caactgcttt ccggtacggc
     5341 gctggcggcg gggattggtt ttgtaaacct gtttggtgca gtgggtgggt ttattgcccc
     5401 gatcctgcgc gtgaaagcag aaacgttgtt tgccagcgat gcggcgggat tactgacgct
     5461 ggcagcggtg gcggtcatcg gttcgctgat tattttcact ctgcgtgtaa atcgcactgt
     5521 tgcgcagacc gacgtggcac atcattaata ggttaaggaa cacgatgaac gcattattaa
     5581 ccaatccctt taaagaacgt ttacgcaagg gcgaagtgca aattgggctg tggttaagct
     5641 caacgactgc ctatatggca gaaattgccg ctacttccgg ttatgactgg ttgctgattg
     5701 acggggagca cgcgccaaac accattcagg atctttatca ccagctacag gcggtagcgc
     5761 cctatgccag ccaacccgtg atccgtccgg tggaaggcag taagtcgctg attaaacaag
     5821 tcctggatat tggcgcgcaa actctactga tcccgatggt cgatactgcc gatcaggcac
     5881 gtcaggtggt gtctgccacg cgctatcctc cctacggtga gcgtggtgtc ggggccagtg
     5941 tggcacgggc tgctcgctgg ggacgcattg agaattacat ggcgcacgtc aacgattcgc
     6001 tttgtctgtt ggtgcaggtg gaaagtaaaa cggcactgga taacctggac gaaatcctcg
     6061 acgtcgaagg gattgatggc gtgtttattg gacctgcgga tctttctgcg tcgttgggct
     6121 acccggataa cgccgggcac ccggaagtgc agcgaattat tgaaaccagc attcggcgga
     6181 tccgtgctgc ggataaagcg gctggttttc tggctgtggc tcctgatatg gcgcagcaat
     6241 gcctggcgtg gggagcgaac tttgtcgctg ttggcgttga cacgatgctc tacagcgatg
     6301 ccctggatca acgactggcg atgtttaaat caggcaaaaa tgggccacgc ataaaaggta
     6361 attattgata tcaaaggccc atgggggtcg gctgtgggcc tgtgttaatt agtggttatt
     6421 cgctgccaga tcggcttcgc ttagctgggt ggctgcgagc accaggtcac gatcaatgcc
     6481 ctgtaccagc atgcgcaaag caattttaat ggcttgttta cgcatacctt cctgcaaacc
     6541 ttctaattta ccctcctgcc agccttcaag cttaccttcc tgccagccaa tctgatgccc
     6601 ttcctgcctc agccgttcag caatagtcat caatctctcc ttttaatgac agttcggaat
     6661 atcagcatca gtgagttggg ttgtcgccag cacaatctca cgttcaaagc cctgctccag
     6721 catgcgcaag gcaattttaa tggcttgttc atgcatgcct tcctgcaaac catctaattt
     6781 accttcttgc aaaccttcta atttaccttc ctgccaacct tcaattttac cttcctgcca
     6841 gccttcaatc ttaccttcct gccagccaat ttgatgccct tcctgccgca gccgttcagc
     6901 aatagtcatt aatctctcct tttgtagtgg tgaacgttcg gcaatctcct caataaaacg
     6961 ggtgaaacgg gaggtatcgc cgcattgcag cagataatta aacagcgttt gtagctggct
     7021 gtcattagtg aagcctctaa ccaaaagcgt ggtgatcctg tcgaccatac cgattaaatc
     7081 gcggtcgcga atatgctttt gaatcagttc aagcagagct atacgccgat gttgcatgat
     7141 ctcatcgtca ggtacgatgg tgatatccac caacggaaaa gcttcggtgt acaactgccg
     7201 ggcaagttgc ggatcgtcaa actcatccag ccagttgagt gagtacgggt agggcgaggt
     7261 ttcgccatga taaaacagca atggcaccac cagcggaact ctgtcatagc ctttatccag
     7321 gtgacgctgc atggcggcag tggcatagcg cattagccga aaagccatat tcttttctgc
     7381 agagctttga tgttcaatca cgcagtagat atagccttca ctgtcgctgg tttccacaga
     7441 ccacaaaaca tccgagtagt aagcgcgtaa acttttttca ataaagctgg tgggttccag
     7501 gcgtaaagtt tgcaggttgc aaagcttgcg cagcggctct ggtagatgta tttccagaaa
     7561 atcccgagcg gtttcgggtg tgaacataaa ggttttaaac accgcatcat gcggggagga
     7621 ggttgttgat tcggtcatgg ttgtccgtca cctgatattt atgatgacgc gacattacgg
     7681 ttatcccagg cctttgacat ctgccagttt cagcctttgc gagacgtttt cagaggaatt
     7741 ttcactgcgt tgcaacgcat acattgggca cggaatcgtg tacgcaaaaa tgcccggtaa
     7801 caacatgctt accgggcatc agaaaggagt ttaagccagc gcctgggcca gcagcgtaat
     7861 cggatgttcg cagcgaagac ttgtggacat ctcaatctgc catttgcagg tttcgcagtc
     7921 ggtaaccacc agatctgcgc cgctttcttc tatctgacgg aacagtggtg cgccgatggc
     7981 ttgtgaggtg gggtagttct cttttttgaa accgtaagta cctgcaatac cgcagcactg
     8041 ggaatccagc accgttaact caagccccgg gattttacgc aacagctcca gggtgtagag
     8101 cgtccagccc attttttcca tatggcacgg agtgtgataa accactttca gcggcagcgg
     8161 tttcagcggc aaggttttgc cttcgtccag cttgcgccac agccagcggg tcgccagttc
     8221 gatgtgatcg cgcaagcctt tgttgtcgac attcagcact tccggatatt cgtcgcgcag
     8281 ggcaaatgta caggttgagg aggtggcaat caccggaatg ccttttactc ccactgcttc
     8341 gcggatcgac tctacatttg tgattgcctg tttacgtgct ttatcggtaa agccgttggc
     8401 gatcagcggt acgccgcagc atttttcttt gctgagcagt tgtacaccgg tacccattgc
     8461 gttgagcact ttaattaaat ctttgcctaa ctgcggatgg ttgtagttaa cgaagcagcc
     8521 gtgaaagaaa gccacctggt ctttatattg tgcctgctgg gccgccacgc tgcgatacca
     8581 gcgacggaac gtaccgaagg agtatttcgg tagcgtgcgg cgatgatcga tttttaacgc
     8641 cgcatcaagc agctgccgca ctggtttcag cgaggtagcg gtattgacga ttggtgcgaa
     8701 tggcgtggaa acgctaccca tcaggtcggt atgactcaac acaaaattgc gcagcgacgg
     8761 gcgtgtggtg tcatatttcg cccgcgcgcg ctggatgata tcgccaatct tcacatcgga
     8821 cgggcaggcg acttcgcagc gtttgcagtt gatgcaatat ttcagcgctt cgtcatacag
     8881 tgcgccatct ttcagacgca gacgctcgcc atccggcccg gcttgttttg gccctggata
     8941 gccgggattc acccggctca ccgggcaggc ggtggtgcag acggtgcact taatgcagtt
     9001 ttcgaagctg gtgtcgttca tagttggcct cctgcgcgtt gggcaatctg ttgagcggca
     9061 tgtaaagcac tgacggcaca aacaccgccg ccgcatccct gggcgatggg atcaaatccg
     9121 cccagcaccg aaccgatggc aaacaggttc tcaatggttt gccctgcctg cgatgggcgt
     9181 agtgcctcat cagtggttac accgaacagc tgccacggtt gcggcgcaaa aaaatcgccc
     9241 ttataccatt caccccgcgt ggcggtttgt agcacatcaa ggccgagaat cggctcgcga
     9301 atgccgtcac gttctgccac cagtccgccg ctaaagaaac tgccgctcgc aagcaccgca
     9361 aatcgtgggc gcagcgggat atcggcgtga ttgcgcgtcc agatttcatt cactacgcca
     9421 tttttacagg tcactttttt cacttcatcg cctggcatcc acacgccacc ctggcgcaca
     9481 aactggcgct gtaactggtt ttgcagacga atgcccagca cggaaggcgg cagcgttggc
     9541 aaaagcatca gtgaacaagg tagtttttca ttcaaccaac gccacagttt gtcatcggcc
     9601 aggccgaagc aggcgggcat caggatcatt tcgcaggtat tggcgacagg aataagcgca
     9661 tcaagtaaca gcgaccagtt ctcttcgtta tcaaggaaac gggcgatatt caccgcgcga
     9721 aactcggtgg cgttgttgcg cagcacatcc agttccggca gctctatttc tgcggtttca
     9781 acggcaaggt cgagttcgcg caacgaagcc gccgcaagat gcgcctgaaa atccatcagg
     9841 ccgcttatcc cgaccacaca tattttcttc gcgggcaacg gccagacagg aacttctggc
     9901 gaacttagcc aggttgagcg cagagtgccg agcggggtaa ctcgctggtg ggccagttct
     9961 acgctgccct gtaattgcgc accgctttca gcgattaatg cctgcgcctg gcaagcgaga
    10021 tcgagtacgc gttgtggccc gagaagagtg taaggatggg caggtgcctg ctgacgcaaa
    10081 gcttccagtc cactgtgaat gtctgtcacc ggttgaccat ccggcagatg gctcagcaaa
    10141 tccagcgatc cagatgagaa atggagcgcg ctttgaccac gcgtgacaat ggcacagcgc
    10201 aggccgtgtt tttgcagttg caggccacag agtaatccgg cgaggccgcc gcccataatg
    10261 acagtatcaa agcgcatctt tctgctcctt ctccagacca cacagtccct gataaaccca
    10321 gcgggtaaat tcgctttcgc gcagtgcatc tccccaggcg atgggttgca cgcctttcca
    10381 gcgttcgtta aggaaggtgg aaagttgctc gatagattgc gcggacatcg tgacgttaaa
    10441 acgttgcagc agtccggcag cgcggcaggc gcagagttcg ccctggcagg tacccatccc
    10501 cacacgggta cgacggcgta aatccagcag gctattaacg tttaaatttt ctaccgcgta
    10561 ctgaacttca cccgcagtta ccgcttcgca ctcacatacc aggctacggt gcagacggcc
    10621 ttcgctcagc caggcgggtg tgcgatcgcc atgacgataa accgcagaac cgcgcagcgg
    10681 ggcaggcagg gagatgactt tacgcaaggt aacttcagcg ggctcctgtg aaccaggaag
    10741 tgccagatcg gcggttgtac aggggcgcgt gttgcccagc ttgcgacata ccgcgtcggt
    10801 agcccattcc gccatcagcc gataggtcat cagtttgccg ccggtgatgg tgataaatcc
    10861 gtccagacca tcgcgttcag catggtcgag cagcacgatg ccacgactga cgttacggcc
    10921 gctcgggtcg tcatcgctgg caaccagcgg acgcacgcca gagtaagccc gcaaaatacg
    10981 cgttttcgcc atcacggggg ccagtttttc cccttcacgc agcagaatat caacttcttc
    11041 tgccgtcact cgattatcgt caatctcatt gtaatcaata cgtaaagagg tggtgccaat
    11101 tagcgaaatg gtatcgccag gcaccagaat atcggcgtcg gaaggtttgc ggcagcggtt
    11161 gatcacatgc tgattaatgc ggtgatccat gatcagcagc gatcctttcg ccgggaacat
    11221 gcgaatgcgc agatcggcat attcggcaat gcgttgcccc cagatcccag cggcattaac
    11281 cacgacaggc gcatgaaggg cctgagtttc gccggtgaga tggttacgta cacgaacgcc
    11341 gcacaccgtc gcaccttcac gaatcagccc cgtgacttca tgagcggtaa ggataacggc
    11401 accgtgttct ttggcatcca gcatgtttgc tgcggtcaga cgaaatggat caacggtgcc
    11461 atccggaact ttcaccgcgc caatcagtgc cgggttaacg gcaggttcga taatgcgcgc
    11521 ttgctgcggg tctatagctt ctgcgctgat ccctgcttct tcgcaggcgc ggataaaagt
    11581 ggcctggaag gagaggtcat cttccggcag ggtgataaac aggccgttgg tcggttcaac
    11641 gcagtgacgt gcaatgcgtt tcaggatctg gttttcacta atacattcgc gggccgattc
    11701 cgcatcggtt accgcatagc gcgcaccgct gtgcagcagg ccgtggttac gcccggtggc
    11761 accggttgcg atgtcgtggc gctcaaccaa aatcacgcgc agtccgcgca gggcacagtc
    11821 gcgggcaatc ccggctcccg ttgcgccgcc gccaatgata atcacgtcac ttgattgcga
    11881 gtcgcgagtt ttcattgtta tccctctgaa gttcgttttt taccatttag ccatagtaaa
    11941 aacatgaatt gtttgatttc gcgcatattc gctcataatt cgaaagttaa acgtgatttc
    12001 atgcgtcatt atgaacattt aacccatcga atttaataat gtgtgcggta attcacattt
    12061 aatttatgaa tgttttctta acatcgcggc aactcaagaa acggcaggtt ctcccactga
    12121 atcaggctgt taatcataaa taagaccacg ggccacggag gctatcaatg ttgagtattt
    12181 ttaaaccagc gccacacaaa gcgcgcttac ctgccgcgga gatcgatccg acttatcgtc
    12241 gattgcgctg gcaaattttc ctggggatat tctttggtta tgctgcttac tatttggttc
    12301 gtaagaactt tgccctcgct atgccttatc tggtcgagca gggattctca cgcggtgatt
    12361 taggttttgc cctttcgggg atctcgattg cttatggatt ttcgaaattc atcatgggct
    12421 cggtgtcgga tcgctcgaat ccgcgcgttt tcctgcccgc aggtttgatt ctggcggcgg
    12481 cggtgatgct ttttatgggc tttgtgccgt gggcgacgtc gagcattgcg gtgatgtttg
    12541 tgctgctgtt cctctgcggt tggttccagg ggatgggatg gccgccgtgc ggtcgtacta
    12601 tggtgcactg gtggtcgcag aaagaacgtg gcggcattgt gtcagtgtgg aactgtgcgc
    12661 ataacgtcgg tggtggtatt ccgccgctgc tgttcctgct ggggatggcc tggttcaatg
    12721 actggcacgc ggcgctctat atgcccgctt tctgcgccat tctggtggca ctgttcgcct
    12781 ttgcgatgat gcgcgatacc ccgcaatcct gtggcctgcc gccgatcgaa gagtacaaaa
    12841 atgattatcc ggacgactat aacgaaaaag cggaacagga gctgacggcg aagcaaatct
    12901 tcatgcagta cgtgctgccg aacaaactgc tgtggtacat cgctatcgcc aacgtgttcg
    12961 tttatctgct gcgttacggc atcctcgact ggtcaccgac ttatctgaaa gaggtgaaac
    13021 acttcgcgct ggataaatcc tcctgggcct acttcttcta tgagtatgcg ggcattccgg
    13081 gcaccctgct gtgcggctgg atgtcggata aagtcttccg tggcaaccgt ggggccaccg
    13141 gcgtcttctt tatgacactg gtgaccatcg cgactatcgt ttactggatg aacccggcag
    13201 gtaacccaac cgtagatatg atttgtatga ttgttatcgg cttcctgatc tacggccctg
    13261 tgatgctgat cggtctgcat gcgctggaac tggcaccgaa aaaagcggca ggtacggcag
    13321 cgggctttac cgggctgttt ggttacctgg gcggttcggt ggcggcgagc gcgattgttg
    13381 gctacacagt ggacttcttc ggctgggatg gcggctttat ggtaatgatt ggcggcagca
    13441 ttctggcggt tatcttgttg attgttgtga tgattggcga aaaacgtcgc catcaggaat
    13501 tactgcaaaa acgcaacgga ggctaatggc atgaaattga cgctgaaaaa ccttagcatg
    13561 gcgatcatga tgagtggaat gatcatggga agcagtgcaa tggcggcgga cagcaacgaa
    13621 aaaatagtca tcgcccatcg cggtgccagt ggatatttgc cggagcatac gctgccagca
    13681 aaagcgatgg cgtatgcgca gggagcggat tatctggaac aagatttggt gatgaccaaa
    13741 gacgaccatc tggttgtcct gcatgaccat tatctcgatc gtgttactga tgttgccgat
    13801 cgtttcccgg atcgggcgcg caaagacggt cgttactacg cgatagattt cacgctcgat
    13861 gaaattaagt cactgaaatt taccgaaggt ttcgatattg aaaatggtaa gaaagtacag
    13921 acttatccgg ggcgtttccc gatgggtaaa tctgacttcc gggtacacac tttcgaggaa
    13981 gagattgaat ttgttcaggg gttaaatcac tctaccggga aaaatatcgg tatctatcca
    14041 gaaattaaag cgccgtggtt ccatcatcag gaagggaagg atattgcggc aaaaacgctg
    14101 gaagtgctga agaaatatgg ttacagcggt aaggacgaca aagtttattt gcaatgtttt
    14161 gatgccgatg aactgaagcg tattaagaat gagctggaac ctaaaatggg catggacctc
    14221 aatctggtac agctgattgc ctataccgac tggaacgaaa ctcagcagaa acagccggac
    14281 ggaagctggg ttaattacaa ctacgactgg atgtttaagc cgggggccat gaaacaggtg
    14341 gcggaatatg ctgatggtat tggtccggat tatcatatgt tgattgagga gacatcgcag
    14401 ccaggtaata tcaaactcac tggcatggtg caagatgctc agcagaacaa gctggtagtg
    14461 catccttata ccgtgcggtc agataaactg cctgaataca ctactgatgt gaatcagtta
    14521 tatgatgctc tgtataacaa agcgggtgta aatgggctat ttactgattt ccctgataag
    14581 gcagtaaaat tccttaataa agagtaaatt aactaccgga tggcacttat gtgctatccg
    14641 ataccactct tcattaaagt ctgtgggtta tccttaattg caattatttt gagcgagatg
    14701 acttgttccc ggcataagat actcgtcaat acaacacgta ttattaagat tttcctcagc
    14761 taaacatcta tagtcattgt cgttaataaa aaacgacagg cgatagggta tggcagtttc
    14821 agcaaagcac gacgaattta atcactggtg ggcaacagag ggcgactggg ttgaagagcc
    14881 taactatcgc cgtaatggta tgagcggggt gcaatgcgtc gagcgcaacg gcaaaaagct
    14941 atatgtaaag cgcatgacgc atcatctgtt tcattccgta cgttatccgt tcggccgacc
    15001 aacgattgtc cgtgaggttg cggtaattaa agaactggaa cgggccggtg tcatcgtgcc
    15061 gaagatcgtt tttggtgaag cggtgaaaat tgagggggaa tggcgcgcgt tgctggtgac
    15121 tgaagatatg gcggggttca tcagcattgc tgactggtat gcccagcatg cagtaacgcc
    15181 ctattctgac gaagtacggc aagccatgtt gaaagcagtg gcgctggcgt ttaagaaaat
    15241 gcatagcgtt aatcgtcagc atggctgttg ctatgttcgc catatctatg tgaaaacaga
    15301 aggtaagtca gaagctggtt ttcttgatct ggagaaaagt cgtcgccgct tgcgtcgcga
    15361 taaagcgatt aatcatgact tccgccagct ggaaaaatat ctggagccga tccctaaagc
    15421 ggactgggag caggtgaaag cgtattacta cgccatgtaa tcgttgtagg ccagataata
    15481 cgcgtaagcg tcgtatctgg cgctagtacc cgatcacaac tcgatttcaa tatcgccttt
    15541 tgcccggcaa caacagggca aaatttcccc cggctgaata aaggctaacg gttcggcaat
    15601 ccagtccacc tggcctgcaa caaggcgcgt gcgacaggaa ccgcagtaac cttcgcggca
    15661 ctggtactca accgccacat tgtgggattc cagcgccgcc agaagggaag ggtgttcatc
    15721 ctggcacagc agttgtgtgc cagtgatgcg cagggtaacg cgagccatca gagctggaag
    15781 ttactcaaat cgtcggtgtc cacttccgag tcaatctgcc cgaccagata agaactgact
    15841 tccacttcct gcggagcaac ctgcacgtta tcagacacca gccaggtatt gatccacggg
    15901 atcgggttgg agcgtgtctg gaacggcaga tccaaaccga ctgcctgcat gcggatattg
    15961 gtgatgtatt caacgtactg gcagagaatg tctttattca gaccaatcat cgaaccgtca
    16021 cggaacagat aatccgccca gtctttctct tgctgtgccg cctggacaaa caggtcatag
    16081 cactcctgct tacattcttc ggcaatttcc gccatctccg gatcatccgc gccgctgcgc
    16141 agcagattca gcatatgctg agtaccggtc aggtgcaggg cttcatcgcg agcaatcagg
    16201 cgaataattt tggcgttacc ttccatcagt tcgcgttctg caaaggcaaa ggaacaagca
    16261 aagctgacgt agaagcggat cgcttccagc gcgttaacgc tcatcaggca gagatacagt
    16321 tttttcttca gctcgcgcag gctaacggtt acggttttac cgttaacggt gtgggtacct
    16381 tcgcccagca gatgccagta gctggtcatt tcgatcagct catcgtaata gctggagatc
    16441 ccttccgcac gtttctggat ctgctcgttg gtgacgatat cgtcaaacac aacagacgga
    16501 tcgttaacga tattacgaat gatatgggta taagaacgag agtgaatggt ttctgagaac
    16561 gcccaggttt cgacccaggt ttccagctca gggatcgaga tcagcggcag cagtgccacg
    16621 ttcgggctgc gaccctgaat ggagtccagc agcgtctgat atttcaggtt gctgataaag
    16681 atgtgttttt cgtgttcagg tagtgcctgg taatcgatac ggtcacgaga aacgtcaact
    16741 tcttccggac gccagaagaa agagagctgc ttttcgatca gcttttcgaa gatgtcatat
    16801 ttttgctgat cgtagcgagc cacgttaacc ggctgaccaa agaacatcgg ttctttcagc
    16861 tgatcatttt tcgtctgtga aaaggtggta tatgccatga atgtgtcctg ttgggagttt
    16921 aatgccggat aaggcatttt acgccgcatc cggcatctca atatcagatc ttacatgcgc
    16981 cgctttcgca gccatcgtcc tggattgacg gtaccagatc gtcttgtgcg tcttcagcac
    17041 cgtcacgggt gttctgataa tacagcgttt tgaccccgaa tttgtaggcg gtgagcaggt
    17101 ctttcagcag ttgctgcatc ggcacttttc ctgacgggaa gcgtgacgga tcgtagttgg
    17161 tgttagcaga gatcgactga tcgataaatt tctgcatgat acccaccagt tgcaggtagc
    17221 catcgttacc cggcatttcc cacagcagtt cataggcgtc gtgcagatgc tcgtagtccg
    17281 gcaccacctg gcgcagaata ccgtctttcg acgctttgat gctgacgtaa ccacgcggcg
    17341 gttcaatacc gttagtggcg ttagagatct gcgaagaggt ctcggacggc atcagggcag
    17401 aaagcgtgga gttacgcagg ccgtgcgttt tgattgactc acgcagtgct tcccagtcgt
    17461 aatgcagcgg ctcattagcg atggcatcca gatctttctt ataggtatcg atcggcagga
    17521 tccctttcgc gtaagtggtt tcgttaaacc acgggcacgc gccttgctct ttcgccagct
    17581 cattagacgc tttcagcagg taatactgaa tggcttcgaa ggttttatgc gtcaggttgt
    17641 tggcgctgcc gtcggagtag cgtttaccgt gcttcgccag gtagtaagcg aagttgatca
    17701 caccaatacc cagcgtacga cgacccatcg ctccacgttt ggcggccggg atcgggtaat
    17761 cctgataatc cagcagcgcg tcgagtgcgc gtactgccag aattgccagc tcttccagtt
    17821 catccaggct gttaattgcg cccaggttga aagcagacag cgtacacagc gcgatttcac
    17881 cgttctcgtc gttgacgtcg ttcagtggtt tggtcggtag ggcgatttcc aggcacaggt
    17941 tagactggcg cactggtgca atagccggat caaacgggct gtgggtgttg cagtggtcta
    18001 cgttctgaat atagatacga ccggtagacg cacgttcctg catcatcagc gagaacagct
    18061 caacggcttt cacacgctgc ttgcggatgc tgtcgtcttt ctcatatttg gtgtacaggc
    18121 gttcaaactc ttcctgatcg gcgaagaacg cgtcgtacag ccccggtacg tcggacgggc
    18181 tgaacagggt gatatcttca cctttcagca gacgggtata catcagtttg ttgatttgta
    18241 ccccgtagtc catatgacgc acgcggttgc cttcaacccc acggttgttt ttcaacacca
    18301 gcaggctttc cacttccaga tgccacatcg ggtagaacag tgttgccgca ccgccgcgca
    18361 caccgccctg agagcaggat ttcaccgctg tctggaaatg tttgtaaaac ggaatacagc
    18421 cggtgtggaa tgcttcgcca ccgcgaatcg ggctacccaa cgcacgaata cgcccggcgt
    18481 tgatgccgat cccggcacgc tgggaaacgt atttaacaat cgcgctggag gtggcgttga
    18541 tggaatccag gctgtcgccg cactcgatca gtacgcagga gctgaactga cgagtcgggg
    18601 tacgcacgcc ggacatgatt ggcgtcggca gcgaaatttt aaatgtggaa accgcgtcgt
    18661 aaaaacgctt cacgtattgc aggcgcgttt cacgcgggta gttcgagaac aggcacgcgg
    18721 caaccagaat ataaaggaac tgggcgcttt cgtagatttc gccggtcacg cggttctgta
    18781 ccagatattt gccttccagc tgcttaacgg cagcataaga gaaggtcata tcacggtcgt
    18841 gatcgataaa cgtgtccatc tgcttgaact cttcttccgt gtagtcttcc agcagatgat
    18901 tatcgtattt gcccatctcg accattttca ccacgtggtc gtacagcgca ggcggctcaa
    18961 actggccgta ggctttttta cgcaggtgga agatcgccag gcgcgcggcg agatactgat
    19021 aatccggcgc atcacgggag atcaggtctg cggcagcctt gataatggtt tcgtggatgt
    19081 cagaggtctt gataccgtca taaaactgaa tgtgggagcg cagttcgacc tgggaaatcg
    19141 aaacgttatg cagtccttct gccgcccaat ccagaacgcg atggattttg tcgagattga
    19201 tgcgctctgt gctaccgtcg cgctttgtca ccagcagatt ctgattcatg tatgtcgtac
    19261 ctgtttttgg aattctttcc gcaatagttt catgcagact acctgtagtg agggaagctg
    19321 cttagaacac tatatatagg gggtatgttt gggattcact gcaagatagt gtgaaaatgg
    19381 ccctcttgca agtgcataac tttgtggata actcaggaag gaaaaagtgg ctttcgcgca
    19441 ccttaggtca gacaaggtgt ccgggaaagt caatgggaag agaaaaattt gttaaaaata
    19501 actgttcggt gatttcttga acggcagaat aggttggata aattgatata gatggtttat
    19561 taatcttttt gctaatgatt gcaaaaattg atctaagcac gagtcatcac ataatctgat
    19621 gtatcagttg tggtcaatat ataagcgaat gtaagcgatt gttgatgtcg aaatggttgt
    19681 tttctatgcg gttattgtta aatactaagc caatttgtta cttttgtatt tattataaga
    19741 atattattta ttgctttaat
//
LOCUS       DAZGHF010000086        19544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGHF010000086 DAZGHF010000000
VERSION     DAZGHF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.086"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..160)
                     /locus_tag="ACHK3A_003217"
     CDS             complement(<1..160)
                     /locus_tag="ACHK3A_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO8741266.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVL"
     gene            280..480
                     /locus_tag="ACHK3A_003218"
     CDS             280..480
                     /locus_tag="ACHK3A_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741267.1"
                     /translation="MKLDISVKYFLNIMIADFIIPAPEKISTTWHSDTKMNRFACPIH
                     STNSDYYSGFYHTDVLIPGELA"
     gene            520..702
                     /locus_tag="ACHK3A_003219"
     CDS             520..702
                     /locus_tag="ACHK3A_003219"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741268.1"
                     /translation="MFFNWNVLFYVSLEKFPVGNPYYGEIPGIPVDEEANFFNPFPPR
                     AFENKPLYTEEITKYD"
     gene            695..1561
                     /locus_tag="ACHK3A_003220"
     CDS             695..1561
                     /locus_tag="ACHK3A_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8741269.1"
                     /translation="MIKGIMFAFLAAFSWGAAIVMSKKGLKNMDAGELFFWQVGSAAM
                     LSWFVLAISRKKIPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITSVIFSLESV
                     FILILSVIFFSVKINSLLHFILLLTGAMAGSIMVALPDINDRIDNITGYILIVAGVLS
                     ASFYVVISSRLIVNFEPVTLLTGQLTFSFTLACFFIVLTDTSLQLPIESVFLVFLSGI
                     LQYFLAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVFITIL
                     CVYILNKKYAIE"
     gene            2046..2651
                     /locus_tag="ACHK3A_003221"
     CDS             2046..2651
                     /locus_tag="ACHK3A_003221"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO8741270.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIVAWNNVIWGVATLLLLWTIFNRCRSALREEGVK"
     gene            2648..3565
                     /locus_tag="ACHK3A_003222"
     CDS             2648..3565
                     /locus_tag="ACHK3A_003222"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO8741271.1"
                     /translation="MTLLEKSLVVIFSLLLIATVINGLLVWLRPGKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPADGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            3569..4210
                     /locus_tag="ACHK3A_003223"
     CDS             3569..4210
                     /locus_tag="ACHK3A_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO8741272.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRVRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLTQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTER
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQKLTALLQPSQGNHPHD"
     gene            4203..5963
                     /locus_tag="ACHK3A_003224"
     CDS             4203..5963
                     /locus_tag="ACHK3A_003224"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO8741273.1"
                     /translation="MTNSRIPDERQFLTSDNTELFYRHWPAQEASSTPKVVVLFHRGH
                     EHSGRLQHIVDELAMPDTAFYAMDARGHGRSPGLRGYSPSLVRSVQDVDEFVRFAAAD
                     SQAGMTDVVVVAQSVGAVLAATWAHDYAPDIRGLVLASPAFKVKLYVPFARQGLELMY
                     RLRGLFFVNSYVKGKYLTHDPERVASFNKDPLITRPIAVNILLDLYKTTERVVSDASA
                     ITLPVQLLVSGEDYVVHRQPQLDFYQNLRNPQKELHVLPGFYHDTLGEKERHLAFDRM
                     RSFIDTLYARPVQRFDYSQEDRWSPGADTWRILSGGPALYSLDDISYRCLRYGMKVLG
                     TQSTGVRLGFETGFDSGSTLDYIYRNQPQGSTSLGRLIDKNYLNSVGWKGIRQRKIHL
                     QMLIQQAVARLTEQGSAVRIVDIAAGHGRYVLDALQGETAVSEILLRDYSELNVAKGQ
                     EMIASRGMAGRARFEQGDAFNRNELAALEPRPTLGIVSGLYELFPENGPVRDSLAGLA
                     AAIEPGGILIYTCQPWHPQLKTIAWSLTSHKNGKAWVMRVRTQGEMDALVRDAGFEKC
                     TQLIDEMGIFTVSMAVRRQP"
     gene            5960..6231
                     /locus_tag="ACHK3A_003225"
                     /pseudo
     CDS             5960..6231
                     /locus_tag="ACHK3A_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6253..6509)
                     /locus_tag="ACHK3A_003226"
                     /pseudo
     CDS             complement(6253..6509)
                     /locus_tag="ACHK3A_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6787..7395
                     /locus_tag="ACHK3A_003227"
     CDS             6787..7395
                     /locus_tag="ACHK3A_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO8741274.1"
                     /translation="MGKDLEEKKNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATKS
                     VSSGISSPAVETHIPEAPARKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRAEG
                     GGFLPLKTGITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRYNL
                     DGQPEGEVSECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
     gene            complement(7616..9100)
                     /locus_tag="ACHK3A_003228"
     CDS             complement(7616..9100)
                     /locus_tag="ACHK3A_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8741275.1"
                     /translation="MRKIIVHFKIVLTLLLPATVSAQSIEWQSCMTTPFSNWFGEEKP
                     SPDLLCGYLSVPLKYTDTGEDSSSVKRQQVKLALTKLPAKSRHKGSIIIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSDTGIQENITEKQRVLNKI
                     NSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAYSYGTQIAALYAERFPSS
                     IRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSVRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDALGVILCVDQGGEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEIDIK"
     gene            complement(9409..9813)
                     /locus_tag="ACHK3A_003229"
     CDS             complement(9409..9813)
                     /locus_tag="ACHK3A_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFO8741276.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHAWDDYVDNFFR"
     gene            complement(9981..10268)
                     /locus_tag="ACHK3A_003230"
     CDS             complement(9981..10268)
                     /locus_tag="ACHK3A_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8741277.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASERMR"
     gene            complement(10265..10516)
                     /locus_tag="ACHK3A_003231"
     CDS             complement(10265..10516)
                     /locus_tag="ACHK3A_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO8741278.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            10969..11925
                     /gene="iroE"
                     /locus_tag="ACHK3A_003232"
     CDS             10969..11925
                     /gene="iroE"
                     /locus_tag="ACHK3A_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HFO8741279.1"
                     /translation="MYASEYRSTRPRKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSVTSFDSVDDTRHYRVWTAVPNKTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAEGRKTDPHSGHFSRKSGGSNDF
                     RHLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDTWLSSSYFRLYYSASPSLGRG
                     NDALLSRVTAVEPLQFCAKHLAIMEGSATPGDNRETHAVGVLPKIHTTLTILKDKGVN
                     TAFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(12427..13218)
                     /locus_tag="ACHK3A_003233"
     CDS             complement(12427..13218)
                     /locus_tag="ACHK3A_003233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741280.1"
                     /translation="MSRLVNIVAGMLPFLALLPLSGCASRPDYALSPSGNTEWVTVAV
                     KLPPETEALPVDVLYRSEICRRKDYDPTTESHVRTIRGFNPVQIPLSPPDNSGFRKVR
                     IALDGGTRCGWTLSGVRVGIQMSRSSALAQGKDVTAANYVFDFDDEGYGSAFGKGKPL
                     SVSGDLFFKTEFFPLVYINHMFNETYLELFAGNAKYEKWNRYYRVRETENIAIEPVLY
                     VNKVVRVESAKGKENGPGMIVSYPDASTEKIKDIYPDYTKLLSMK"
     gene            complement(13202..15253)
                     /locus_tag="ACHK3A_003234"
     CDS             complement(13202..15253)
                     /locus_tag="ACHK3A_003234"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HFO8741281.1"
                     /translation="MGIEIIGDNTTITSIKPKGKVVYCRGESHKYWLELQFVDENNVP
                     VTGLNVWLEYHPLASAQEVAMWKKYGNDYNPTPPPNPPAGVTDNQGVVRFDNLYWIAV
                     DVKTDAQPLTDEMEKRPLGLRRNPNSQPVSNNRFRPETRDPEWRSDVQEKAEAAGYIH
                     HYVTIGELCDQLPEIEGWTEPEPPKFHFPHGTLLKGTEITREALDKRHVIEICPFRAW
                     SLVLHDTKDYSLANGYNLGVMADMAYADKVLIEDFFRRKCLDLSTTPRHEVHPFYPHA
                     LVVDVPFRERYTTVEFLDSRSQKDKAPSRLLIDSTQLFYVENASHVIVAWRGTQEVPD
                     WVTDGSYSSQPCPPELAGAGRIHGGFWDAYHLVKVRFEDDFKKIKLTLANANGAKKLF
                     IAGHSLGGALGLIYAAEMKVVEPILYTYGMPRTFTADAVGNLYQITHYRHVNDSDVIA
                     TVPFDANLDNWLYGKFGPLGSTLGFFWTLLVELPVQMTGAYTGEHFWHHGKPVVFFRA
                     TQTGSYEECKTMNSHTCRRLSFSQQYKIKLFLVPSLAEVENEEAKEAQEAFVREFPVE
                     DIQRIFPRNTNPRMDHMPSPFRHSMAGEYLPFLNNQLLESAGAEQELQRKIYREDFRQ
                     QMKQFGDSAPPEEMKRNRIFLALQDMVGNTLDQTRKMPGGENALIRFKSVAEEEVESS
                     R"
     gene            15374..15619
                     /locus_tag="ACHK3A_003235"
     CDS             15374..15619
                     /locus_tag="ACHK3A_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741282.1"
                     /translation="MKRRKLTPAEEKRLADAFVATAIAMGLKGNKKLPQEIKRRVRAQ
                     VLSAIENERKAQAAHDARRQEFEAGENTFTWQPLRRR"
     gene            complement(15711..15989)
                     /locus_tag="ACHK3A_003236"
     CDS             complement(15711..15989)
                     /locus_tag="ACHK3A_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741283.1"
                     /translation="MKKCVTVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLEQQKKSLR"
     gene            16110..16532
                     /locus_tag="ACHK3A_003237"
     CDS             16110..16532
                     /locus_tag="ACHK3A_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO8741284.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFHTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            16930..18048
                     /locus_tag="ACHK3A_003238"
     CDS             16930..18048
                     /locus_tag="ACHK3A_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8741285.1"
                     /translation="MAKLYLYLVLAASLSALSTDMIVPALPELQKAFNADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDYFGKVRVMTICLIIFLIGSIVCFMANNLNMLLLGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSAIIPLIAPLAGALILDYYNWNVIF
                     IVMGAFSILTILLSPKESNKQEKKTHQDNVNFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMSDYGLNTFNYSVVFSLSVLFFIIGNQLSKIEKISNPWILFPLNAISVTPFLIL
                     NDAFIICVIGAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAGTISFL
                     SVKISDNGVSLGTTLSISCLILALLSIFVTIIIGNNKK"
     gene            18045..18875
                     /locus_tag="ACHK3A_003239"
     CDS             18045..18875
                     /locus_tag="ACHK3A_003239"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO8741286.1"
                     /translation="MNWQKKKIERSIKYQTRVFNEHINELVITRRNGKVITVNDGREM
                     IEFVSCSYLGLETHENLKHAIANAIERFGVQLSVARTRVKVDLFEQLEERLNKIMNGA
                     HTLTFNAVTPCHLAVLPLLASGTLPNYKFNKIPYFILEKTAHATLQINRGLLQQFGEI
                     IRIDFQHKNDIEKAFKYASINNMIPISISDSVGSMGGVAPVEFIVRMANRFDGYAYID
                     DAHGTSIYGKNGSGYVMSCLDNKLDDRIIIAGSLSKAFGSHGGLVLMVALLHAIILTP
                     "
     gene            18903..19256
                     /locus_tag="ACHK3A_003240"
     CDS             18903..19256
                     /locus_tag="ACHK3A_003240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741287.1"
                     /translation="MLLVVHHHYQELLHVSQQQIYTSMAQYPCVKPCVKMTYNNINKG
                     TTMPIRGLLIGNEEKAITICKKMHDRGFATTVAMYPTVEEGKAILRCAISAIHTRENI
                     DSLHKNFIECINELG"
     gene            complement(19326..>19544)
                     /locus_tag="ACHK3A_003241"
     CDS             complement(19326..>19544)
                     /locus_tag="ACHK3A_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741288.1"
                     /translation="DADTPYPPEPHLMQFRLKGCQDTLFAAINKPEEMPDYLFRQLGF
                     NQTWHEWKRDEQHRQQQRRPGHYRGMSM"
BASE COUNT         5237 a         4522 c         4357 g         5428 t
ORIGIN      
        1 tcagcaccgt gacctgccag aacggcataa agggtaatgg cggaagccgg atacccagcc
       61 gccagagaat acgcagaaga ggaggggcat aattgctccg ccacatcttt ttgctgtcca
      121 tcagggcaat ggcccgggct tttttctgct caaaggtcac aggagcacct cacgttccat
      181 gatgtaaacg tgacacagct tattgttatt gattattatt atcatgctgt gattatgtta
      241 tcatgcaccg gtacacgata acaataaagg agaaaccaga tgaaactgga tatatctgta
      301 aagtattttc tgaatattat gatagcagat ttcatcattc ccgcaccaga aaaaatatca
      361 accacatggc actcagatac taaaatgaat cgctttgctt gccctataca ttctactaat
      421 agtgattact attcaggttt ttatcataca gatgtattaa ttcccggaga actggcttag
      481 gacgccatca taatccatga tgttgatagt aatgatccta tgttttttaa ctggaatgta
      541 ttgttttatg tctcactgga aaagtttcct gttggcaatc cttattatgg agaaattcca
      601 ggtattcctg tggatgaaga agcaaatttt tttaatccat ttccaccaag agcgtttgaa
      661 aataaacctc tttatactga ggagataact aaatatgatt aagggaatta tgtttgcatt
      721 tcttgctgct ttttcatggg gagcagcaat agtgatgagc aaaaaaggtc tcaaaaacat
      781 ggatgccgga gagttgtttt tctggcaagt tggtagtgct gcaatgttat catggtttgt
      841 tctcgcaatt agcagaaaaa aaatcccggt aacaaaaaaa tcaatacttg cctattcaac
      901 aggaattttt gagccatttc tggcgtatac attcactctc tatggcctaa aatttatttc
      961 tgctggtata acatccgtta ttttttcact tgagtcagta tttattctta tactatctgt
     1021 tatattcttt tccgttaaaa ttaactctct attacacttt atattattgc ttactggagc
     1081 aatggcaggc agtataatgg tcgccctacc tgatatcaat gaccgcattg ataatattac
     1141 aggatatata cttattgttg caggtgtact ctcagcatcc ttttatgttg ttatctcatc
     1201 acgacttatt gtgaattttg agcctgttac gttgttaacc gggcagttga ctttctcttt
     1261 tactcttgct tgttttttta ttgttctaac tgacacatcc ctgcaactgc caatagaatc
     1321 tgtatttctt gtgtttttgt caggaatttt acaatatttt ttggcttttt gtttctatct
     1381 ttattcccta cgctggattc ccgttcacat agcaggagca atgctctatt ttattcctct
     1441 ggtagcattg ctgttgtcct ggttcttcct gcaggaagat atatcattta ttcagggact
     1501 aggcgttttt ataactattt tatgcgtata tattctcaat aagaaatacg ccatcgaatg
     1561 actttattag ggttgcgtta tcgcggtagg gtacaccctg acaccataac caaaaacgtg
     1621 atgccagacg atgaatgaca taaccttaat gcgataaagt ggtaaaaaat gttcatcaga
     1681 gctgatttaa aagaagtaaa gcaatagccc ggaccagcga caaggcatca tgcaacaaat
     1741 gtaacggata ttttgaatat ccttgcctcc gcacatgact gggcataatt aaagaaaagc
     1801 gtatgctgac acagctgcac agagtgctgt accgttgtcc aggacactat gtttatggtt
     1861 cagccacaca gtctaccggc acaggtactg gctctcaggc ctcccgtaat accagtcagt
     1921 cgcctttacc ggcgcagcag gcaggatttc cgcgcccgga actgtattca ctttcagtgt
     1981 gccagtgaca gccccaataa aaggccagac tcaaccccgt caaatatgct tattaaggag
     2041 tgatgatgac cctttaccag ataaagccct cgtttcaggc gctgttaaga ccgctgatgt
     2101 tctggctgaa caggcgcggc gtcaccgcta atcacattac actgtgcgcg atggtgcttt
     2161 cccttatcac cggcgtggtg ctcattattc atcctcagcc cggattattc gtccttctgc
     2221 ccgtggtgtt gtttgtacga atggcgctca atgcgctcga tggtatgctg gcgcgtgaat
     2281 gtaatcaaag atcgcgtctg ggggccatcc tcaatgagac gggtgatgta atttccgaca
     2341 tctcgcttta tctgcccttt atgctactcc cgcagagcag tataccgctg gtgctgatcg
     2401 tggtgttttg tgcgctgatg accgagtttt gcggcgtgct ggcgcaaacg attaacggcg
     2461 tgcgcagcta tgccggtccg ctgggcaaaa gcgacagagc gctggtgttt ggtgcatggg
     2521 ggctgtcact gtcgatctgg ccgcaaattg tcgcctggaa taatgtcata tggggtgtcg
     2581 caacactgtt gttgttatgg acgattttta atcgctgtcg cagcgcattg cgtgaagagg
     2641 gtgtgaaatg acgctacttg aaaaatcact ggtcgttatt ttttccctgc tgttgattgc
     2701 caccgtaatc aacgggttgc tggtgtggct gcgtccaggt aaagactggc gcgagctgac
     2761 attacgtatt cgcacctggt gggtgattat cgtcctgttt tccttcgcac tattaagtcc
     2821 tagctggctg gcactgacgt tcttcgcgct ggtcagcttt atggcgttaa aagagttttt
     2881 gacgctggtg ccttcccgcc agtccgaccg catgccgctg ttatggatgt ttattgcaat
     2941 tccccttaac tactggctga taggtatcgg ctggtacggc atgtttgtgg tgtttatccc
     3001 ggtctacgtg ttcctttttt taccggcacg aatggtgatt gctggggata cacagggctt
     3061 tttacgtacc gtttcccagt tgcactggag tctgatgacc accgtatttg ccttcagcca
     3121 tgtcgcgttt ttactggtgt taccggcgga tggaatgcaa accagcgcgc tgttggtgct
     3181 gtttttggtc ggcctgaccg aatttaacga catcgcgcaa tacctgtggg gcaagtcgct
     3241 tggtcgtata aaagtcatac cgaaggtcag cccaaacaaa accctggagg gattgctggg
     3301 tggggtggca acgacggcgc tggcggcaac agtcctgggg ccactgttga ctcccctgag
     3361 ctggccgatg gcactgttag ccggggttat tatcggcgtt actggctttt gcggcgacgt
     3421 ggtgatgtcg gcaatcaagc gtgatatcgg cgtcaaagac agtggtacgc tgctacccgg
     3481 ccatggaggc atccttgatc gactggattc gctgattttt accgcaccgg tcttcttcca
     3541 ttttgttcgc tatttctgct actaacctat gatgacacga ctactccgta ccctgtttac
     3601 gctgtgtatc gtctggccgg tcatctggct gtggcttggt ctgcgtgtcc gccaccgcga
     3661 gcgactgccg aagcacgggc cttcgataat cgtggcaaat cacaacagcc atatggatgt
     3721 tttcgccttg ctttcgcttt ttccgctgcg ttaccaggct tcagttcatc cggttgctgc
     3781 cgcggattat tttctgcggg ataaacgcat ggcttggttt gcgctgaaga tccttaacat
     3841 cattcctatt tcaagaaagg gcggttatac cgatccgctg acgcaatgtg aacaggcgct
     3901 gcgtgaaggt aaaacactga tcctgtttcc ggaagggacg cgcggtgagc caggcgtact
     3961 tgcgccattg aaatccggag tgtggcattt gagtcagcgc atgccggagg tgcccattat
     4021 ccccgttggg ttgcgcggta ctgagcgtgt gatggcgaaa ggtaatcgta tccctttgcc
     4081 gctgtttatt gatgtaaata ttggcgatgc gctaagtacg catccggaga agaaaacgtt
     4141 tatggacgac ctggagcaaa aactgacggc gcttttgcaa ccctcgcaag gaaatcatcc
     4201 ccatgactaa ttcccgcata cccgatgaac ggcagtttct gaccagtgat aatacagagc
     4261 tgttttatcg ccactggcca gcacaagaag ccagttcaac gccaaaagtg gtggtcctgt
     4321 ttcatcgtgg gcatgagcac tccgggcgtt tgcaacacat tgttgatgag ctggcgatgc
     4381 cggatacagc gttttatgcc atggatgccc gaggtcatgg tcgctcaccg ggtctgcgtg
     4441 gttacagtcc gtcgctcgtg cgttcggtgc aggatgttga cgagtttgtg cgtttcgccg
     4501 ccgcagatag ccaggccggg atgacggatg tggtcgtcgt ggcgcaaagc gtcggggcgg
     4561 ttctggcggc aacctgggcg catgattatg ccccggacat tcgtggcctg gtgttggcat
     4621 caccggcgtt taaggtcaaa ttgtatgtgc cgtttgctcg ccaggggctg gaattgatgt
     4681 atcgcctgcg cggtcttttc ttcgtcaact cgtatgtgaa aggcaagtat ctgacgcacg
     4741 atcccgagcg ggtggcgagc ttcaataagg atccgctgat tacccgacca attgctgtga
     4801 atattctgct tgatctctac aaaacgactg agcgagtcgt cagcgatgcc agcgccatta
     4861 cgctacctgt gcagttgctg gtctccgggg aagattacgt tgtgcaccgc cagccgcagc
     4921 ttgattttta tcagaacctg cgaaatccgc agaaagaact gcatgtactg ccgggatttt
     4981 atcatgacac cttaggcgaa aaagagcggc atctggcatt tgacaggatg cgcagtttta
     5041 ttgatacctt gtatgcccgt cccgtgcagc gttttgatta cagccaggaa gatcgctgga
     5101 gccccggcgc cgatacctgg cgcatactga gcggtggccc tgcgctgtat tcgctggacg
     5161 atatctctta ccgctgtttg cgctacggga tgaaagtgct gggcacgcag tccacggggg
     5221 tgcgtctggg atttgagact ggtttcgatt ccggcagtac cctagactac atctatcgca
     5281 accagcctca gggcagcacc tcattggggc gcctcattga taaaaactac ctgaatagcg
     5341 tgggctggaa ggggatccgc cagcgtaaaa ttcatctgca gatgttaatt cagcaggctg
     5401 ttgctcggtt aacagagcag ggtagcgcgg tccggatcgt ggatattgcc gccgggcatg
     5461 gacgctatgt gcttgatgcc ctgcaaggcg aaacggcggt aagcgagatc cttctgcgcg
     5521 attacagcga attaaacgtg gcgaaaggtc aggagatgat agcctctcgc ggtatggcgg
     5581 gccgtgcgcg atttgagcag ggggatgcct ttaatcgtaa tgaactggcg gcactggaac
     5641 cgcgtccgac gctggggatc gtgtccggtc tgtatgaact gttcccggaa aacgggccgg
     5701 tacgagattc gcttgccggg ctggctgcag cgattgaacc gggaggcatt ctgatctaca
     5761 cctgtcagcc ctggcaccct cagttaaaaa ccatcgcctg gtcactcacc agtcacaaaa
     5821 atggcaaagc ctgggtaatg cgcgtacgta ctcagggcga gatggacgcg ctggtccgtg
     5881 acgccggttt tgagaaatgc acgcaactaa ttgatgaaat gggcatcttc acggtctcta
     5941 tggcggtacg acgtcaacca tgacaacgcg gcgtgcggtg attattcagg gaatcggctg
     6001 gctaatggtg ctggccccgt tcttctttct tacttatggc atgacgaatc agtttaccgc
     6061 cgggagagaa aacatcagca gcctggtgtt tgactgggag caccggatcc cgttaatacc
     6121 gctgacgatt attccgtact ggagtctgga tttgctttac tgtttatctg cgtttcactg
     6181 aatgagcagc ggcgtctggt ctgtcggctg gtgttggctt cccttattag tacaataatt
     6241 atctatatcc aaactggaca gataaaaagt tcccgtgttg tttttaagag cagcaaaatt
     6301 catggtcggc gaatatcccg gaatacagtc acactgtaac catgcatcat actcacgcat
     6361 ttcccggaga agatcattgg ccagagcatg tagtgccgta gttgctgttt tttcagtccg
     6421 cagaccgcct ttactgccat ctcttgctca cagctaagct cccgaatcac tttgctccgg
     6481 ggattcttct ttacaagctt cattggcata atgaatgcac tccttatata catacttaaa
     6541 cagcaagtaa aatatacaaa gaacatccca tgaagcaaag gcagagatag actgtgcgcc
     6601 ccgatatatt cttctgatat tttgctttat ttacttcaaa ttaatttact tctgtcagaa
     6661 ccacaacaaa gtggatacaa tatatttttt agaggccgat tattaaacaa atttatcaga
     6721 tgagaatctt tattaattta taatttgtga tattattaaa aaataaattc tgaagcagag
     6781 gtatttatgg gaaaagatct ggaagaaaag aaaaatcccg tcattgtggt gaaaaaacgg
     6841 cgctcttttt caccgccttc cttagccgaa aaaaaagatg ttatcgcgcc tgcactcact
     6901 gaacaggcga caaaatcagt ctcctccgga atcagttcac ctgccgtcga aactcatatc
     6961 cccgaagcgc cagccaggaa gaagaaaaag aaaagacaac gctttccaag accatcccac
     7021 tggaccagtg agttcactca cgattgtctg gagaaggtga aagcgctgtt tcctcaactt
     7081 cgtgccgaag ggggcggttt tctgcctctg aaaacaggca taacccgtga cattactaca
     7141 tatctggcag cacatccgga aacagcgctg accctggacg aatggaattg tgcagtatcc
     7201 tgcattacca gtcgccggat ctacctgcag agaacctctg ttgcaggaac tccccgttat
     7261 aacctggatg gtcaaccaga gggggaagta tcggaatgtg atgctcaaaa ttccaggcgc
     7321 tggctggcgg cgaaagagca gaggtggctg agtatgaagg cccggcagga aaatatttct
     7381 gagaaatcaa aataaccgca ggatattaca ggaaacagac tgtagagaag agagcgttgt
     7441 atattgtgcg tattatttaa gcactcccga gagtaatggc ttgattttgc gcgatcgcga
     7501 cattttttac gtgtatccag tttgacgttt ctggtgccgt gatcgcactg aacgcatttt
     7561 gctcccaatg caaaatatgt cctgtatcag gagcaaatgg cgggcaattt ttaaactatt
     7621 ttatatctat ttcagcattt cctggacagg atatatttcc cattttcata ggggtgataa
     7681 gatgacgaac aacggcctta tctacacata aattgactcc ggtaagagct aatgtatgcc
     7741 catcaccatt tattgtcagc aacgggctgg aaaatttctc tgccatctta cgagcattaa
     7801 tccatggtgt tgttggatcg tatttgtgtg ctacaaacag taaaccagag ggcagaactg
     7861 tatttttcag ccgggtttta ttcaggttgc tatgtattgg ccataattca caaaaatcag
     7921 gtgaatcgga acgtccattg tcaaagttaa tagccgggaa ggcattcgca agagcgtctt
     7981 ttcgggattt tcgctcttct ggggttaatt gctccccccc ctgatcaaca caaaggatta
     8041 cccccagagc atcacttgac tcttctgagg ctataggagc actgagcgca gtttcaattt
     8101 cattactgac aatcccctga gagaactgac gtatggcagt tgcaagggtt ggccatgatg
     8161 aacgccataa caaaaggtct gttgttaata atatgagttc atctgaagat atattttctc
     8221 ccttgctgtc taataaaggc ttgtgatgca attttgataa cagctcatgg aactgagtta
     8281 ttgccttatc tctgactgaa gaaagtgggc aactttttgt acgtgcacac caggatgcaa
     8341 agcgatcgaa cgtttcctga taactctgtg cttgcttgag ttgccatgtg aagttgtccc
     8401 ccaggtcatc aatatcgacg accccatcaa gaacgataga tctgatgctg gatgggaaac
     8461 gttctgcata taaggctgca atttgtgttc catacgaata tgccacagct gtcagttgct
     8521 cgtcacccag ggcttgcctg atacgatcaa tatcatatac cgcctcgtta gaaccaatat
     8581 ggcgaataac ttcagttccg gtattatgga tacagctgtt aattttattt aatacccgtt
     8641 gcttttcagt tatgttttcc tgaatccctg tatctgattg ctggcagttt attgcaggaa
     8701 tggactggcc gactccccgt ggatcaaagc caataatatc ccatgattcg cgaagatttg
     8761 ttaccggcca gtcaaagtta atataaggat taatgccagg taatcctggt ccaccactta
     8821 ttataatgat actcccttta tgcctgcttt ttgctggcaa ttttgtcagc gccagtttta
     8881 cctgctgtct ttttacagaa gaactatctt cgcctgtgtc tgtatatttt aatggaacag
     8941 acaaataacc acatagtaag tcaggagacg gtttttcctc accaaaccag ttactgaatg
     9001 gagtagtcat acaggattgc cactctatcg actgggcaga tactgttgcg ggcagaagta
     9061 aggttaaaac tatcttgaaa tggacaatta tttttcgcat tgtgtctctt aatatcggta
     9121 taaaggtaaa gtttgaatat cttaaatccg gtgttacggc aggaagttac acgttacgga
     9181 tataattcaa atgattacaa agtcagggta tattgtttaa ctccccggaa ctatgtaagc
     9241 gtcaccgtct tgtgacgtct tatgatggtt attctgtctg gtaacaatcg gttattttcc
     9301 tggtctccca ctgacagcaa aagaacaaga ctgctgaact ataaacggcc atgatgaatc
     9361 atcacgattc ggccccagac ttgtccgtca ggtaattttt cggttcagtc accggaagaa
     9421 attatcgacg taatcatccc atgcatgccc ctgaacagac cattcatgat taaccggtgg
     9481 cgggaatcca cttcgacagt agccgttctt cccctcaaaa tcgaataccc catgtggata
     9541 tccgtttatc agcagaacac acttcaggca atcctcagac cagccaatct tcacatcaga
     9601 ggggatgtga gcatctgaaa tgtcttcaac gttatatata tgcacagcat ccctgacagg
     9661 gttaccttca acggactcat caagtgcata gaaatatccg gtattcccgt cgtcttcaaa
     9721 tacagcggcc aacattccat caggagctaa actttttatc gccatagcct caccaactat
     9781 gagttgagcc tgtgccgtca caagtattgt catcaaacat gtccctccgt ttcaatttgt
     9841 ctggtgatat aaataacccc agactcatcc cggcaggtaa aatcattctc tcaacaaaac
     9901 ttttcttttt ccgcctcagg cgaggccaca gagagaataa caattctgag agttatctgc
     9961 gggaaaggct atttatgtta ctatctcatt ctctcactgg cgagataata aacgttacta
    10021 cgttctcgtt tacctaccgc aacaaccgca ataattaaca tatcatcaat cacctgataa
    10081 accagacgaa atcccgatgc acgaagttta attttgtagc agtcctttag cccacgtagt
    10141 tttgcagacg gtatatgagg atttttgcta cattttttta gttttttcac aaactgttgt
    10201 tgaatggtct tatccagttt cagccattct ttaagcgcat catccctgaa ttttatcgta
    10261 taagtcatga ataactgtcc aggtcaacat catgcagagg ttgactgcta cgttcagtaa
    10321 ccagtttcac cagctcctgg tcatccagag catcaagcat cctttcatac agttcagcag
    10381 gaacacagta gaatgccggc tgattgcgat tcagaatggc gaccggataa ccatcaccag
    10441 caccgactgt cgccatcgga ttttttttca gttcactgac acttgcactg atgtcagaca
    10501 gaatgatgtt aggcataatg caccttttaa gactgtttaa taagtcttaa agatacatct
    10561 gaaaggaccc gttaacaagt ctgtattact catacatttg caaataaatt taatgctgat
    10621 tttttactct gatgccgcac cagcgcatgg aaggtagcac gctgtggttt acatacatat
    10681 tattcgtcca ttaaatttaa taaacagtcc tgaaccccgt aaggctatct cagcctagta
    10741 tacgataggt tttatttaac catttccagt gcagggataa tgtcatgggg ataaaaatta
    10801 tcgtgcgcaa tctacaccgg tggtcacgat tacacgtggt ggcacggtgc actgattgac
    10861 gggattggtt tactacaggg ttgagttgac ccacaaacac tttcaggaaa cggtacagac
    10921 gtcctgaata aatcaaattg tcacctgcgg aaaaggaata atcatcagat gtatgccagc
    10981 gagtatcgct caacacgccc gcgtaaagcg attttctttc atctttcctg tcttaccctt
    11041 atctgtagtg cgcaagttta tgcaaagccg gatatgcgac cactggggcc gaatatagcc
    11101 gataaaggct ccgtgtttta ccatttcagc gtcacctctt tcgactctgt cgatgacaca
    11161 cgccattatc gggtatggac ggccgtgccg aataaaaccg caccggcatc gggttacccg
    11221 attttatata tgcttgacgg taacgcagtt atggaccgcc tggatgacga actgctcaaa
    11281 caattgtcag aaaaaacacc gccagtgatc gtggctgtcg ggtatcagac caacctccct
    11341 ttcgatctca acagcagggc ttacgactat acgccagcag cagaaggcag aaaaacagat
    11401 ccccactcag ggcattttag ccgtaagagt ggtggcagca acgacttccg ccacttacta
    11461 gaaacgcgta ttgccccaaa agtggaacag ggattgaata tcgatcggca gcgccgcggc
    11521 ttatgggggc actcctacgg cggcctcttc gtgctggata cctggctctc ctcttcttac
    11581 ttccggttgt actacagcgc cagcccatcg ttgggcagag gtaatgatgc tctgctaagc
    11641 cgcgttacgg cggttgagcc actgcaattc tgcgccaaac acctggcgat aatggaaggc
    11701 tcggcgacac cgggtgataa ccgggaaacg catgctgtcg gtgtgctgcc gaaaattcat
    11761 accaccctaa ctatactgaa agataaaggc gtcaataccg cattttggga tttccccaac
    11821 ctaggacacg ggccgatgtt caatgcctcc tttcgccagg cactgttaga tatcagtggt
    11881 gaaaacgcaa attatacagc aggttgtcat gagttaagcc actaaacact gcccgctttt
    11941 acgcggacag tacgcctgaa acactacgat cagaatgatg cggtaactgg ggtcagctca
    12001 cgaaacggat atttttgtac gcaaagagat tcattatgac cgtttatagt accgcagtct
    12061 cttcccttta tttttaatga caagcaggca aaaacataaa aactggcgtt gacatgccgg
    12121 ggaagccctt atagtacgtt ttcgtacggt tgggcttcaa accccgtaag acacgactta
    12181 acgccactgg cagcagccat tggtaacggg atagtggatt tggatacgca gaactcttga
    12241 agttgaagcc ttataacgac tacactagaa ggacagcttt tggtatctgt gctccactta
    12301 agccagttac ccggttaagc agttccccaa ctgacttaat cttcgactaa accgcctccc
    12361 caagtggttt tttcgtttac aaggcaagag attacgcgca gataaaaagg atctcaagaa
    12421 gatcctttac ttcatcgaaa gtaacttggt gtaatcagga taaatatcct taatcttttc
    12481 cgttgaagca tctggataag aaacaatcat ccccggacca ttctctttac ctttagcact
    12541 ctcaacccta acaactttat ttacatatag cacaggttct attgcaatat tctcagtctc
    12601 acgaacccta taatatcgat tccatttttc atattttgca ttgccagcaa atagttcaag
    12661 atatgtttca ttaaacatat gattaatata tacaaggggg aaaaattcag ttttaaaaaa
    12721 caaatcaccg ctcacagaga gaggcttccc tttaccgaat gcgcttccat atccctcatc
    12781 atcaaaatca aagacatagt ttgctgctgt aacatcttta ccctgcgcca gagctgagct
    12841 tctggacatc tgaatcccca cccggacacc gctgagagtc cagccacatc gtgtaccccc
    12901 atccagagca attcgcacct tacgaaaacc gctattatcc ggggggctaa gtgggatctg
    12961 taccggatta aatccccgaa tggtacgaac atgtgattcg gtcgtagggt cataatcctt
    13021 acgcctgcaa atttccgagc gatacagtac atccactggc agagcctccg tctccggagg
    13081 cagtttcacc gccactgtca cccactccgt attacccgat ggactcaggg cgtaatccgg
    13141 acggctggcg caaccactga ggggcaacaa tgcaagaaac ggcagcattc cagccacaat
    13201 attaacgaga cgactcaact tcttcctccg cgacagattt aaaacgaatc agtgcattct
    13261 ctccacccgg catctttcgg gtttgatcca gcgtatttcc taccatatct tggagtgcca
    13321 ggaatatccg gttacgcttc atctcttccg gtggtgcact gtctccaaac tgcttcatct
    13381 gttgccggaa atcttcccgg tatatcttcc gctgcaattc ctgctccgct ccggctgatt
    13441 ccagtaactg attattcagg aacggcagat attcaccagc catcgaatgg cggaagggac
    13501 tgggcatgtg atccatccgt ggattagtat ttcgcggaaa aatccgctgg atatcttcaa
    13561 ctggaaattc ccggacaaat gcctcctgag cttctttcgc ttcttcgttt tcaacctcag
    13621 ccagtgacgg aacaaggaat agtttgattt tgtattgctg actgaaactg agacgacggc
    13681 aggtatgact gttcatcgtt ttgcattctt cataagaacc agtctgagtg gcccggaaga
    13741 acaccaccgg cttgccatgg tgccagaaat gttctcctgt atacgcccct gtcatctgca
    13801 ccggtaattc aacaagcagt gtccagaaaa atcccagcgt actgccaaga gggccaaact
    13861 taccgtacag ccagttatcc agattcgcat caaacggcac cgtggcgatc acatcactgt
    13921 catttacgtg tcggtaatgg gttatctggt acagattccc gacggcatct gcagtaaagg
    13981 tgcgcggcat cccgtaggtg tagagtattg gctcaacaac tttcatttct gctgcataaa
    14041 tcaaccccaa tgctccgccc aagctgtgcc cggcaataaa caatttttta gcaccattag
    14101 cattagctaa tgttaattta attttcttaa agtcatcctc gaatcgcact ttcaccagat
    14161 gatatgcatc ccaaaaccca ccatgaatac gcccggctcc agccagttcg ggaggacagg
    14221 gctgtgaaga ataagaacca tcagtaaccc agtcaggtac ttcctgtgtt cccctccatg
    14281 ccactatcac atgggacgca ttttccacgt aaaaaagctg tgtgctgtca atcaacagac
    14341 gggatggcgc tttatctttc tggcttcggg agtccagaaa ctccactgtg gtatagcgtt
    14401 cacggaatgg cacgtccaca accagtgcgt gaggatagaa cggatgtact tcatgtcggg
    14461 gcgtggtgga taaatcaagg cattttcttc tgaaaaaatc ctcaatcagc actttatccg
    14521 cataggccat atcagccatc actccgaggt tatagccgtt tgccagtgaa tagtctttcg
    14581 tatcatgcag caccagtgac catgcccgga acgggcatat ttctatcaca tgccttttat
    14641 caagagcctc ccgggtgatt tccgtacctt tcagcaacgt accatgagga aaatgaaact
    14701 tcggcggctc cggctcagtc cagccctcaa tctccggcag ttggtcgcaa agctctccaa
    14761 tggtcacata gtgatgaata tacccggcag cttcggcttt ctcctgcacg tctgagcgcc
    14821 attcagggtc gcgggtttcc ggtcggaatc tgttattgct taccggctgg ctgtttgggt
    14881 ttcgccgcaa gcccagcggg cgcttttcca tctcgtcggt cagtggctgg gcatcagtct
    14941 tcacgtcaac cgctatccag tacaggttat cgaaccgcac caccccctga ttgtctgtaa
    15001 cacccgccgg tggattgggc ggcggtgtgg ggttgtagtc attgccatat tttttccaca
    15061 tcgccacttc ctgagctgag gccagcggat gatactcgag ccatacatta agcccggtaa
    15121 cagggacgtt gttctcatcc acgaactgga gttcaagcca gtatttatgc gattctccgc
    15181 gacagtaaac aactttacct ttgggcttaa tggaggtgat ggtcgtattg tcaccgataa
    15241 tttctatccc catgacatta tccctgcact aaaaatggtt aaacaaagtc catcataccc
    15301 gggaccgcgc ttaacctgaa tttcctcata tggcacataa tgttacgtta atctgtgcaa
    15361 acaggaggcc ttcatgaaac gcagaaaact gactccggca gaggagaaac gtcttgcaga
    15421 tgcatttgtt gcaacagcta tcgcaatggg cctgaaaggc aataaaaaat taccccagga
    15481 aatcaaacgg cgggtacggg cacaggttct cagtgccatt gagaacgaac gaaaagcaca
    15541 ggcagcacat gatgcacgca gacaggaatt tgaagccgga gaaaacacct ttacctggca
    15601 gccattacgt cgtagataaa aaacatcttc accgcttgaa aacccaccaa aaaaaccaca
    15661 taaaaataaa tggtatcaag accatgatgc ccggggaaaa tacgccccca tcagcgtaag
    15721 gatttctttt gctgctccag agactccagt tttttacgca aatcctctct tttttgggca
    15781 tctctggtgc cagccagctc tgctctcaac tcatcgatct gaagttgtat cttcagtctg
    15841 ttactgaaca ttttctgtct ggcattaaca tccgcaacaa tgccgttttt tgtcttctcg
    15901 gccttttgtt gaaaaacatt gctgtccgca tgactggcaa ccgaagcaga aagaacacta
    15961 aaaagcagga ctgtcacaca ttttttcacg ggattattcc tgactcattg accatcaaat
    16021 cacattgggg taaaccgacg tatgataaga gatactcttc ggaaatataa ctccctgagt
    16081 atcaagatta aaaacgcaag gagatgttta tgagatctgc cgctgccagg ctgcttctga
    16141 tacctctgat aacagcaaca atagctctta caggatgcac accaaagacc agcctggaac
    16201 gacatacccg gcattatgtt tatgcttcag atgatggatt tgatcctaac ttccacaccc
    16261 agaaagcaga caccatacgt atgatgctcc cgttctttca gcagttccgg gatatgggga
    16321 tgaaagacaa agcagccgga gtatcagcag aaacggcaca gcaacgtgta aaagaattcc
    16381 actcagaaaa attttttcac tcactccgga gcacaacaac ctttgctggc agaaaataca
    16441 caaacagcga tatgccttcg ccgaaaaaaa tgaaactaat ggcagacacc atttctgcgg
    16501 tttatctcga tggatacgag ggcagacagt aagggattta ccataatccc ttaattgtac
    16561 gtacccctta aaacgctctc agacgcgttc tgacgcaaaa aacgcaaaaa gacaaaaaaa
    16621 ccaggctaaa cagaaaaaac gcgtcagaat cgttttcagg ggcctgcata actatgcatg
    16681 aaagcgatgc gcgatcggtt tcgaccgttc cgaagggcga acaggtcaaa atgacaagtt
    16741 ttctcccacc ggatggaaga ttctgcacgg ggatttttta tccccttcca tcaacctgaa
    16801 tatgcatctg ggagtaccca ttagtttctg atcaaatact atcgcagtgc tatctcaaaa
    16861 tataaaacat ttcaatggtg taagaatttt aaatatgata aataaagata tttttatcag
    16921 gagttaatta tggcaaaatt atatctttac cttgttcttg ctgcatcatt atcggcatta
    16981 tcaactgaca tgattgtgcc tgcgttacca gaactacaga aagcatttaa tgctgactac
    17041 tcattaattc aactaagtat tagtggattt cttattgtat ttgcatttag tcaactgata
    17101 tcaggaacca taggtgatta ttttggaaaa gtacgcgtaa tgactatatg cttaattatt
    17161 tttcttattg gtagcattgt atgttttatg gccaataatt taaatatgct tcttttgggt
    17221 agaattctac aggcaatagg tgcaggtgcc ggacctgtaa tatcaaaagc aattgctaaa
    17281 gagtcttttc caccactaaa gctaaaaaga gcactttctg acatatcatc aacaagtgcg
    17341 ataataccat taatagcacc tctggccgga gcattaatac ttgattatta taactggaat
    17401 gtaatattta ttgtaatggg tgcatttagc attctaacca tactcctgtc acctaaagag
    17461 tctaataaac aggagaaaaa aacacatcaa gataatgtaa attttgtgac taaagatttc
    17521 atctgcggga caatactcgt ttcactaatg ctaagctcac tattttgcta catatcaata
    17581 tcacctgcac tatttatgag cgattatgga ttaaatacat ttaactactc tgttgttttt
    17641 tccctgtcag ttttgttttt catcattgga aaccaattat caaaaatcga aaaaatatca
    17701 aacccatgga tcttatttcc cctaaacgca atatcagtca caccttttct tatattaaat
    17761 gatgctttta taatatgtgt tattggagca atgattttca atttaactct tggcgcatac
    17821 tatccaatag ctaatttcat ctcattacag ataaacggtt cacgaactgg tttggcagca
    17881 tcaattacag gctttactca aacagtaagc gcagggacaa ttagtttcct ttcagtaaaa
    17941 ataagcgata atggagtttc tttgggaacc actttaagta ttagctgtct tattcttgca
    18001 ttattgtcaa tattcgtaac cattattata gggaataata aaaaatgaat tggcaaaaga
    18061 aaaagataga acgttcaatc aaataccaaa caagagtatt taatgaacat ataaatgaac
    18121 tagtgatcac cagaagaaat ggtaaggtaa tcactgttaa tgatgggagg gagatgattg
    18181 agtttgtgtc atgctcttat cttggactgg aaacacatga aaatctaaaa catgcaatcg
    18241 ctaatgcaat tgaaagattt ggagtccaac tttcggtcgc cagaacgcgc gtaaaagtag
    18301 atttattcga gcaactggaa gagagattaa ataaaataat gaatggggcg cacacattaa
    18361 catttaatgc ggttactcca tgccatttag ctgtactccc acttcttgca tcaggaacac
    18421 tacctaatta caagtttaat aaaataccat attttatttt agagaaaaca gcgcatgcaa
    18481 cattgcaaat aaatagaggg ctattacaac agttcggtga aataatcagg attgactttc
    18541 aacataaaaa tgatatagaa aaagcgttta aatatgcttc aataaataac atgataccaa
    18601 tatctatatc tgatagtgtc ggttcgatgg gtggggtagc tcctgtcgaa tttattgtta
    18661 gaatggctaa cagatttgat gggtatgctt acattgatga tgctcatggc acgtcaatat
    18721 atgggaaaaa tggctccgga tatgtaatgt cttgtctgga taacaaactt gatgacagaa
    18781 ttattatcgc tggttcatta tctaaagcgt ttggttctca tggtggtttg gttctcatgg
    18841 tggctttatt gcatgcaatt atcctgacac cataaacttt ataaaaacgt atggaactac
    18901 ctatgctttt ggtggtccac catcattacc aggaattgct gcatgtatcg cagcagcaga
    18961 tttacacctc gatggcacag tatccttgcg tcaaaccttg cgtcaaaatg acttacaaca
    19021 acataaacaa agggacaaca atgccaatac gtggattgtt aattggcaat gaagaaaaag
    19081 ctataacgat atgcaaaaaa atgcatgatc gtggttttgc tacaaccgtt gctatgtatc
    19141 ctacagtgga agagggaaag gcaattctac gttgtgcaat atcagctatt catacccgag
    19201 aaaatataga tagcctacat aaaaacttta tagaatgtat taacgaactg ggttagtaaa
    19261 tatcatggcc tctttataga gaggccatct atttcatttt ttatggcgta tagctgtccc
    19321 tgacattaca tactcatgcc ccggtagtgt ccggggcgtc gttgttgttg cctgtgctgt
    19381 tcgtcccgct tccattcatg ccaggtctga ttaaatccga gttgacggaa gagataatcc
    19441 ggcatctctt ccggtttgtt gatggcagca aaaagcgtgt cctgacagcc cttaagccgg
    19501 aactgcatca ggtgcggttc cggtgggtac ggagtatccg catc
//
LOCUS       DAZGHF010000087        19460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGHF010000087 DAZGHF010000000
VERSION     DAZGHF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.087"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHK3A_003242"
     CDS             complement(<1..593)
                     /locus_tag="ACHK3A_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO8741289.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPPRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHK3A_003243"
     CDS             complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHK3A_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO8741290.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEEEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHK3A_003244"
     CDS             complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHK3A_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO8741291.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHK3A_003245"
     CDS             complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHK3A_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO8741292.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHK3A_003246"
     CDS             complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHK3A_003246"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO8741293.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLAGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHK3A_003247"
     CDS             complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHK3A_003247"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO8741294.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHK3A_003248"
     CDS             complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHK3A_003248"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO8741295.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRCWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHK3A_003249"
     CDS             complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHK3A_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8741296.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHK3A_003250"
     CDS             complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHK3A_003250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO8741297.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6904..7122
                     /gene="slyX"
                     /locus_tag="ACHK3A_003251"
     CDS             6904..7122
                     /gene="slyX"
                     /locus_tag="ACHK3A_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO8741298.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHK3A_003252"
     CDS             complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHK3A_003252"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO8741299.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHK3A_003253"
     CDS             complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHK3A_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO8741300.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHK3A_003254"
     CDS             complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHK3A_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO8741301.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVTVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHK3A_003255"
     CDS             complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHK3A_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO8741302.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10553..12466
                     /gene="yheS"
                     /locus_tag="ACHK3A_003256"
     CDS             10553..12466
                     /gene="yheS"
                     /locus_tag="ACHK3A_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8741303.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12466..13488
                     /gene="yheT"
                     /locus_tag="ACHK3A_003257"
     CDS             12466..13488
                     /gene="yheT"
                     /locus_tag="ACHK3A_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO8741304.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNNLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13482..13700
                     /gene="yheU"
                     /locus_tag="ACHK3A_003258"
     CDS             13482..13700
                     /gene="yheU"
                     /locus_tag="ACHK3A_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO8741305.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13754..14623
                     /gene="prkB"
                     /locus_tag="ACHK3A_003259"
     CDS             13754..14623
                     /gene="prkB"
                     /locus_tag="ACHK3A_003259"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO8741306.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHK3A_003260"
     CDS             complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHK3A_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO8741307.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15384..16016
                     /gene="crp"
                     /locus_tag="ACHK3A_003261"
     CDS             15384..16016
                     /gene="crp"
                     /locus_tag="ACHK3A_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO8741308.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHK3A_003262"
     CDS             16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHK3A_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO8741309.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHK3A_003263"
                     /gene_synonym="dapC"
     CDS             complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHK3A_003263"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO8741310.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
BASE COUNT         4767 a         5334 c         4839 g         4520 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtca
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac ggggcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      481 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      541 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      601 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      661 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      721 cttcaggaat tccatggtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      781 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      841 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      901 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      961 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
     1021 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
     1081 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
     1141 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
     1201 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1261 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1321 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1381 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1441 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1501 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1561 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1621 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1681 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1741 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1801 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1861 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1921 caggtaatcg attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1981 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttaatctc
     2041 ttcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     2101 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     2161 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     2221 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2281 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2341 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2401 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2461 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2521 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2581 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2641 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2701 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2761 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2821 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2881 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2941 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     3001 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     3061 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     3121 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     3181 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3241 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3301 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3361 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3421 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3481 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3541 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3601 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3661 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3721 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3781 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3841 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3901 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3961 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgagaatgat atcgttcgaa
     4021 atttgaccag ccaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     4081 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac accatcttgc
     4141 aataatagca gttcgtcgcc ctcacttagc aggcgcagca gcgcagcaaa atccgtcagc
     4201 cagggtgaac gatgtaatgt gtgcagcata aacgcctcaa aacctcaaaa taacatcgta
     4261 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4321 tgtctgcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4381 gtcgtacaga cccaacaatt taaaagtggc gatgtaatca cgcgccagca ctgcatcggg
     4441 cttttgtcct ggcagcagct gaaaaacgcc atcagcaata aagaagacgg ccagatcgtc
     4501 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cgaccagcag ctgtaccatg
     4561 aggcacagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctatcacag
     4621 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccgg ctgaaggttt
     4681 gacgaagcca gccccagtct tccggcctcc gtttcatcga cgataccgcg gcgtaacgct
     4741 gccgctacgc agatattcag cgccacacca tgttgcgcat tcaactgttg ccagcagcgc
     4801 acaaggtcaa attcatcgct tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4861 cgataaaaaa agacgctgct taactcatgg ccttctgcaa tcagcgcctg cgcaaactga
     4921 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4981 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     5041 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcatcttta atatcgaaga
     5101 tccctttctc gtagagattc agcacgatct gacgattttt ggcgttatta gaaacattgc
     5161 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     5221 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaggtgctca
     5281 taatctgcga gaagggaaca tcaagattca tattgatgca cagcaaacca attacacgct
     5341 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5401 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5461 ccaggtcggt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5521 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5581 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5641 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5701 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5761 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5821 cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg ttcttaatgc ttatttttta
     5881 gcagaatctg cggctttcgc atcagcttcc ggctttgcat cagccttcgg cgctggtttc
     5941 acatccagca gctctacgtc aaacaccagg gtagaattcg gtgggatccc cggaacaccc
     6001 gctttgccgt aagccagttc tggtggaata accagtttga tcttaccgcc tttcttgatg
     6061 ttcttcagac cttctgtcca acccggaata acaccgtcca gacggaaaga gagcggttca
     6121 ccacgggtgt aagagttgtc gaactcttta ccgtcgatca gcgtaccttt gtagttcacg
     6181 acaacagtat cgctgtcttt cggtgcttcg cctttaccgg cttcgactac ctgataaacc
     6241 agaccagttg aagaggtttt cacacctttc tctttggcaa atttctcgcg gtactcttta
     6301 cctttcgctt cgttatctgc cgcgtctttt tccatcttcg cctgagcaga agacttcacg
     6361 cgagcttcga atgcttgcag tgtctgttcg atctcttggt cggagagttt gctcttatca
     6421 gcaaatgcat cctgaacacc agcgatcagc tgatctttat ccagtttgat gcccagtttt
     6481 tcttgttctt taagagaatt ttccatgtaa cgacccagcg aagcacccag tgcataagct
     6541 gatttctgat cgtcattttt gaacgctgct ttgctgtcag cagttgtagc aggttttgca
     6601 gcttcagcag caaaagtgat cggtgcatgc agggcaacgg ccattgtggt cgccagcagc
     6661 gttactttaa acagtgattt catccatatc tccaggatcg gggcatctca ccccagggtt
     6721 aactattatc agaagggtac tataaagcgt tgtcgaacaa atctacatac agacacgccc
     6781 tattatcatc tattttcaga ctctttttgt ttaaattagt ttcgatgacc gcgaaatgag
     6841 tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga ccattcgaca aaagaggtga
     6901 atcatgcagg atttatcatt ggaagcacgc ctggcagagc tggagagccg actggctttt
     6961 caggaaatca ccattgaaga actgaacgtc acggtgaccg ctcatgaaat ggagatggcg
     7021 aaactgcgcg atcatctgcg tctgctgacc gagaagctaa aagccagcca gccgtcgaac
     7081 atcgcgtcgc aggctgaaga aacgccaccg ccacattatt gaggcgtaaa aaaagcgggg
     7141 attccccgct tttttgtcac tttttcggta ttagtggcaa ccgcaaccgc cgttaccttt
     7201 accgccgcag cagccttcgc caccgtgttc atgaccgtga tcgtggccat gaccaccgca
     7261 gcaaccgtcg tggtcgtgat cgtggtgatg atcgtgcgca ccgtgaacgt ggccatgagc
     7321 cagttcttct tcagtcgctt cgcgaatcgc cacaacttca acgttgaatt tcaggttctg
     7381 accggccagc atgtggttac catcaaccac gacgtgatcg tcttcaaccg cagtgatttc
     7441 aaccggtacc ggaccctggt cggtttcagc caggaaacgc ataccaacct gcagttcatc
     7501 aacgcccata aatacgtctt taggaacgcg ttgcaccagg ttttcgtcgt actgaccgta
     7561 agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca acttcgtgac cttccagcgc
     7621 cgtttccagg ccagagatca gggaaccgtg accatgcagg tagtccagcg gcgcactcac
     7681 cggagactca tcaaccaaca caccgtcttc tgtacgtacc tgataggcca ggctgaccac
     7741 caggtctttt gctactttca tgatatctcc tgagcatggg aagaatagtg gcgcagattg
     7801 tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa actcggcgca tatcgctagt
     7861 ccggatgaaa aatcccgatc acttgctcat ctttgcgaac gtgatcgcgg gcttctttgt
     7921 ccgcttctcg catctgatgt ccgcacttaa cacattcaac aatatcaata ttattttcgc
     7981 gccacatcgc cattgaatcc tgcgcctgac aggccgggca ttttgccccc gcaataaaac
     8041 gttttcggat tgccatcttt accctctact caaattcatc ccagccgtcc agctggcgtc
     8101 gttcttgttg catttcacgc tggaaaatct cttccagttc gcgtcgggct tccctggcgc
     8161 gagaaatttg tacggtgtcg gcatgcattg ggatgagctc ccgcaacatc cgcatatcca
     8221 ggcggcgaaa atgcagttgc gcccgctgcg cctgatgtgg atgcatgcct agcgtgacca
     8281 gcgtcttgcg ccccagctct aacgcactgg agaatgtctc acgagaaaac tgcgtcaccc
     8341 ctgcctgtaa taactcatgc gcctccacac gtccgcgcgc tcgcgcaaga atatgtaaat
     8401 gcggaaagtg ctgctggcat atttccacca gtttcatggt gtcttccggc tcattacagg
     8461 taatgacgat agactcagcg gcctctgcac ccgcagaacg taaaagatct acctgcgtgg
     8521 cgtcgccgta ataaactttg tagccatatt tgcgcatcag gttaacggcg ctgatatccc
     8581 gctccagcac agtaatgcgc attttatttg ccatcagcaa acgaccaatc acctgaccaa
     8641 aacgcccgaa gcccacgaca atgacctgcg gtttatcatc gttgacccac ggtttttcgt
     8701 cttcttcttc cggtccgtta aactggcggg atagccattt atccaccagc ttcatcagca
     8761 acggcgtggt catcatggaa agcgtcaccg tcaccagcaa cagcgccatc tggtcgccct
     8821 gaaacaaccg ttgcgaagaa gcggtagaaa agagcacaaa ggcaaactca ccgccctgac
     8881 tcaacacgcc agcaaactgc atccgctctg agctacgcac gccatataat cgcgccagca
     8941 gatacagcac gagaattttc accgtcacca gcacaaccac gcttatcact acccacaaca
     9001 gatgggtata aagcaccccg agattgagcg acatgccgac agagataaag aacaaaccga
     9061 gcagcaagcc tttgaaggga tcgatagctg tttccagttc gtggcgatat tcgctttctg
     9121 ccagcaacac gcccgcaata aacgtaccga gcgccatcga cagccccagc gcatccataa
     9181 acaatgcgga acccaacacc agcagcagcg tcgcggcggt gaacacttcc cgcacgccag
     9241 aagctgcaat aaaacggaat accggacgca gtaaatagcg cccgccaatc agcatgccga
     9301 caaacgccag caccttcatg ccgatcttca tccagtcgaa atgttcgtct gccgaacccg
     9361 ccaacaacgg cactaacgcc agcgccggga tcactgccag atcctgaaac agcagaaccg
     9421 aaaaacctag ctgcccggat tcgctgcgat tcatcccttt ctcacgcatc agttgcaacg
     9481 ccattgcggt tgaggacatc gcaaggccaa taccaccgac caccgccgcc tgccaggcga
     9541 aatccgtcag catcaataat cccgccagca acgccgcgct taacagcacc tgtgccgcac
     9601 ctacgccaaa aatcgaacgc cgcaattgcc aaagcttgga gggattcaac tccaggccga
     9661 tgataaacat caggaacacc acgccgagtt ccgaaaagtg gaggatctca tcgacgtcgc
     9721 taataaaacc cagcccccac gggccaattg caatccctgc cagcaaatat cccaacacag
     9781 cgccaatacc cagccgcgat gccagcggca ccgcagccac cgccgcgaag agaaacaaca
     9841 ctcctgcgag taaaaaatcg gaaccttcca tcagcggcct cctggagaca gcggatttgc
     9901 cagccagtca ccgtaggctc tggcgtgact cgccagctcc tgtgcgcttt gccgtctcgc
     9961 ccagtaaata atgatgggac tcaaccaatg catccggcac atgcccgccg ctagttcaaa
    10021 ggggcgcaga acatcgctca tcgggtagcg attcaaggcg tcataacggt aagcactttc
    10081 cggctcgccg gtggtaatca cgctacgcca gtactttccc gccagttggt ttcctcccgg
    10141 cccgctggca aaaccacggc ttaatacccg gtccagccac tctttcagta gcgccgggca
    10201 gctataggta taaagaggat gctgaaagac aatcacctcg tgctcgcgca gtaatgcctg
    10261 ctcacggggg atatcaataa aaaaatcggg atagtgcgcg taaaggtcgt gcacggtaac
    10321 attgctgagc tgcgtggccg gtttaagcag tacccggttt gccaccgagt cctgagattc
    10381 cggatgggca tacagcagca aaactttcgc tggctgagac atcatccccc tcccggtatg
    10441 gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc cggtgcggca gctcgcccat
    10501 acattacatt atcataatga taagttaaca tagtctgaac atacggcgcc ttatgattgt
    10561 tttttcctcg ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg ccaccgccac
    10621 catcaacccc gggcagaaag tcggcctggt gggtaaaaac ggctgtggta aatcgaccct
    10681 gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc agctacacct ttccgggaag
    10741 ctggcaactg gcgtgggtga atcaggaaac gccggcgtta ccgcaagcgg cgctggaata
    10801 tgtcattgac ggcgaccgtg aatatcgtca actggaagcg cagctacacg acgccaacga
    10861 acgtaacgac gggcacgcca tcgccactat tcatggcaag ctggatgcta tcgacgcatg
    10921 gagtattcgc tcccgtgccg ccagcctgct gcacggcctc ggtttcagca atgaacaact
    10981 ggagcgcccg gtaagtgatt tctccggtgg ctggcgtatg cgtcttaacc ttgcccaggc
    11041 gctgatttgc cgttcagact tgctgctgct cgatgaaccg actaaccacc tcgatctcga
    11101 tgccgttatc tggctggaaa aatggctgaa gagctatcag ggcacgctga tcctgatctc
    11161 tcacgaccgc gacttcctcg atccgatcgt tgataaaatt attcatatcg aacaacaaag
    11221 catgttcgag tacaccggca actacagttc gtttgaagta cagcgcgcca cccgtctggc
    11281 gcagcaacaa gcgatgtatg aaagccagca ggaacgcgtg gcgcatctgc aaagttatat
    11341 cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc cagagccgta ttaagatgct
    11401 ggagcgtatg gagctgattg ccccggcgca cgttgacaac ccgtttcgtt ttagcttccg
    11461 cgcgccggaa agcctgccta atccgttatt aaagatggaa aaagtcagcg caggctatgg
    11521 tgatcgcatt attctcgact cgattaaact gaatctggtc cccggctcgc gcattggtct
    11581 gctaggccgc aacggcgcgg gtaaatcgac attaatcaaa ctgttagccg gcgagcttgc
    11641 gccagtcagc ggtgaaattg gtctggcgaa agggatcaag ctcggctact tcgcccagca
    11701 tcaactggaa tacctgcgcg ccgacgaatc gcctattcaa catctggcac gtttagcgcc
    11761 gcaggaactg gagcaaaaac tgcgtgacta cctcggcggc tttggtttcc agggcgataa
    11821 agtgacggaa gaaacgcgcc gcttctccgg tggggaaaaa gcccgcctgg tgctggcatt
    11881 aattgtctgg cagcgtccga atctgctgct gctcgacgaa ccgactaacc accttgacct
    11941 cgacatgcgt caggcactga ctgaagcatt aatcgagttc gaaggcgcgc tggttgtcgt
    12001 ttcgcacgac cgtcatttgc tgcgttccac cactgacgat ctctacctgg ttcacgatcg
    12061 taaagtcgaa ccgttcgacg gcgatctgga agattatcaa cagtggttga gcgacgtaca
    12121 aaagcaggaa aaccagaccg acgaagcgcc aaaagagaac gcaaacagcg cccaggcacg
    12181 taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa acccaaccgc tgcgtaaaga
    12241 gatcgcccgt ctggaaaaag agatggaaaa gctgaacgca cagctggcgc aggcggaaga
    12301 gaaactcggc gacagcgaac tgtacgatca aagccgtaaa gcggagttga ccgcctgcct
    12361 gcaacagcaa gccagcgcca aatccggcct ggaagagtgc gaaatggcgt ggctggaagc
    12421 ccaggagcag cttgagcaga tgctgctgga aggccaaagc aactgatggc gcagataacg
    12481 acgaccgatg ccaatgaatt cagcagcagt gctgaattca cccctatgcg cggctttagc
    12541 aattgtcatc tacaaaccat gctgccgcgt ctgtttcgtc gcaaggtgaa attcaccccg
    12601 cactggcagc gactggagtt gcccgacggc gattttgtcg atctcgcgtg gagtgaagat
    12661 cctgcacagg cgcggcataa accgcgttta gtcgtgtttc acgggctcga aggcagtctc
    12721 aatagccctt acgcccacgg tctggttgag gcggcgcaaa agcgcggctg gctgggcgtg
    12781 gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat ttaccattcg
    12841 ggtgaaaccg aagacgccag ctggttttta cgctggctgc agcgcgaatt tggacatgcg
    12901 ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc tcgcctgttt gctggcaaaa
    12961 gaaggtaata acctcccgat tgatgcagcg gtaattgtct ccgcgccatt tatgctggaa
    13021 gcctgtagtt atcatatgga aaagggcttt tcccgcgttt atcagcgtta cttgctgaac
    13081 ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc ccggaacact gccgattaat
    13141 ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg acgatctaat caccgccaga
    13201 attcacggct acgctgacgc tatcgactat tatcgtcagt gtagcgccat gccgatgctc
    13261 aatcggatcg ccaaaccgac gctgattatt cacgccaaag acgatccgtt tatggatcat
    13321 caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg agtatcaact gactgaacat
    13381 ggcggtcatg ttggctttat tggcggtaca ttacttcatc cgcaaatgtg gctggagtca
    13441 cgcattcctg actggttaac aacgtatctg gaggcgaaat catgttgatt ccgtggcaag
    13501 acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt gaaggcaccg
    13561 attatggtga acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa cgccagctac
    13621 agtgcggaga agcggtgctg gtatggtcgg agctgcacga aacggtcaat atcatgccgc
    13681 gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg agataataat gatagctaag
    13741 tcatggagtt actatgtctg ccaaacatcc ggtcattgcg gtaacaggat ccagcggcgc
    13801 ggggaccacc accaccagcc tcgcgtttcg taaaatattc gcgcagttaa atctgcatgc
    13861 agccgaggtg gaaggcgaca gttttcaccg ttacacccgc ccggaaatgg acatggcgat
    13921 ccgcaaagca cgcgacgccg ggcggcatat cagctacttc ggccccgaag cgaacgactt
    13981 cggcctgctg gaacaaacct tcattgaata tggtcagagc ggcaaaggga aatctcgcaa
    14041 atatctgcat acctacgatg aagccgtacc gtggaatcag gtaccgggga cattcacccc
    14101 ctggcaacct ttaccggaac ccactgatgt actgttttat gaaggtttac acggcggcgt
    14161 agtcacgcca cagcataacg ttgcgcagca tgtggactta ctggtcggcg tggtgcctat
    14221 cgttaacctt gagtggattc aaaaactgat ccgcgacacc agcgagcgcg ggcactcacg
    14281 agaagcagtg atggactcgg tagtgcgttc aatggaagac tatatcaact acatcacacc
    14341 gcagttttcc cgcacccatc ttaacttcca gcgcgttccc accgtcgaca cttcaaaccc
    14401 gttcgcggca aaaggtatcc cgtcgctgga tgaaagcttt gtggtgatcc attttcgtaa
    14461 tctggaaggg atcgatttcc cctggctgct ggcgatgttg caaggctcat tcatttccca
    14521 catcaatacg ttagtggtac cgggcggcaa aatgggtctg gcaatggaat taattatgct
    14581 gccgctggtg caacgattga tggaaggaaa gaaaatcgag taactttgct attacgccgg
    14641 ataaaatact atccggcttc acaatgggat agttaaatca cgcggcaacc acttcatacg
    14701 agtgagtaat attcaccgct ttttccagca tcaacgccac tgaacaatat ttctcggcag
    14761 agagatcaac cgcacgtgca accgctgcgt ctttcaggtc gcgaccggta acgataaaat
    14821 gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg gcgttcagag gtcaatttta
    14881 cttcacaatc gaccacatcc tgacgccctt tttgcaggat agaaaccaca tcgatggcac
    14941 tgcaaccccc cgccgccatc aacaccattt ccatcggact tggtgcttta tcgcctgagt
    15001 tgccgtccat taaaatctgg tgaccagagg cggattcgcc cagaaaagtt aacccttcga
    15061 cccacttcac tcgcgcttgc atttttgcta ctccactgcg tcaattttcc tgacagagta
    15121 cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt catgctgaag cgagacacca
    15181 ggagacacaa agcgaaagct atgctaaaac aatcaggatg ctacagtaat acattgatgt
    15241 actgcatgta tgcaaaggac gtcacattac cgtgcagtac agttgatagc cccttcccag
    15301 gtagcgggaa gcatatttcg gcaatccaga gacagcggcg ttatctggct ctggagaaag
    15361 cttataacag aggataaccg cgcatggtgc ttggcaaacc gcaaacagac ccgactctcg
    15421 aatggttctt gtctcattgc cacattcata agtacccatc caagagcacg cttattcacc
    15481 agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg ctctgtggca gtgctgatca
    15541 aagacgaaga gggtaaagaa atgatcctct cctatctgaa tcagggtgat tttattggcg
    15601 aactgggcct gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg aaaaccgcct
    15661 gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta aacccggaca
    15721 ttctgatgcg tctgtctgca cagatggcgc gtcgcctgca agtcacttca gagaaagtgg
    15781 gcaacctggc gttcctcgac gtgacgggcc gcattgcaca gactctgctg aacctggcaa
    15841 aacaaccgga tgctatgact cacccggacg gtatgcaaat caaaattacc cgtcaggaaa
    15901 tcggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag atgctggaag
    15961 atcagaatct gatctccgca cacggtaaaa ccatcgtcgt ttacggcact cgttaatccc
    16021 gtcggagtgg cgcgttacct ggtagcgcgc cattttgttt cccccgatgt ggcgcagact
    16081 gatttatcac cccgatatta actatgcact gcgacaaacg ctggtgctat gtttgcccgt
    16141 ggccgttggg ttaatgcttg gcgaattacg attcggcctg ctcttctccc tcgttcctgc
    16201 ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt tttttcaaac gcttaatcat
    16261 tggtgcgtcg ctgtttgcca cctgtagctt gctgacacag gtgctactgg caaaagatgt
    16321 tcccctgccc tttttgctga ccggattaac gctggtactt ggcgtcactg ctgaactggg
    16381 gccattgcac gcaaaattgc ttcccgcatc gctgctcgcc gccattttta ccctcagtct
    16441 ggcaggatac atgccggtct gggaaccgtt gctcatctat gcgttgggca ctctctggta
    16501 cggattgttt aactggtttt ggttctggat ctggcgcgaa caaccgctgc gcgagtcact
    16561 aagtctgctg taccgcgaac tggcagatta ttgtgaagcc aaatacagcc tgcttaccca
    16621 gcacaccgac cctgaaaaag cgctgccgcc gctgcttgtg cgccagcaaa aagcggtcga
    16681 tctaattacc cagtgctatc agcaaatgca tatgctttcc gcgcaaaata ataccgatta
    16741 caagcggatg ctgcgtattt tccaggaggc gctggatttg caggaacata tttcggtcag
    16801 tttgcatcag ccggaagagg tgcaaaaact ggtcgagcgt agccatgcgg aagaagttat
    16861 ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg ctggccgatg acattcttta
    16921 tcatcgcctg ccgacgcgtt ttacgatgga aaagcaaatt ggcgcactgg aaaaaatcgc
    16981 ccgccagcat ccggataatc cggttgggca attctgctac tggcatttca gccgcatcgc
    17041 ccgcgtgctg cgcacccaaa aaccgctcta tgcgcgtgac ttactggccg ataaacagcg
    17101 gcgaatgccg ttacttccgg cgctgaaaag ttatctgtca ctaaagtctc cggcgctacg
    17161 caatgccgga cgactcagtg tgatgttaag cgttgccagc ctgatgggca ccgcactgca
    17221 tctgccgaag tcgtactgga tcctgatgac ggtattgctg gtgacgcaaa atggctatgg
    17281 cgcaacccgt ctgaggattg tgaatcgctc cgtgggaacc gtggtcgggt taatcattgc
    17341 gggcgtggcg ctgcacttta aaattcccga aggttacacc ctgacgttga tgctgattac
    17401 caccctcgcc agctacctga tattgcgcaa aaactacggc tgggcgacgg tcggttttac
    17461 tattaccgca gtgtataccc tgcaactgtt gtggctgaac ggtgagcagt acatccttcc
    17521 gcgtcttatc gataccatta ttggttgttt aattgctttc ggcggtactg tctggctgtg
    17581 gccgcagtgg cagagcgggt tattgcgtaa gaacgcccat gacgctttag aagcctatca
    17641 ggacgcgatt cgcttgattc tgagcgagga cccgcaacct acgccactgg cctggcagcg
    17701 aatgcgggtg aatcaggcgc ataacactct gtataactca ttgaatcagg cgatgcagga
    17761 accggcgttt aacagccatt atctggcaga tatgaaactg tgggtaacgc acagccagtt
    17821 tattgttgag catattaatg ccatgaccac gctggcgcgg gaacaccggg cattgccacc
    17881 ggaactggca caagagtatt tacagtcttg tgaaatcgcc attcagcgtt gtcagcagcg
    17941 actggagtat gacgaaccgg ggagttctgg cgatgccaat atcatggatg cgccggagat
    18001 gcagccgcac gaaggcgcgg caggtacgct ggagcagcat ttacaacggg ttattggtca
    18061 tctgaacacc atgcacacca tttcgtcgat ggcatggcgg cagcgaccgc atcacgggat
    18121 ttggctgagt cgcaagttgc gggattcgaa ggcgtaatac aggctaaacc atcggatgag
    18181 gcgttcacgc cgcatccgac atcttttgcc tgacgcgtct tatttacgcc ccaatcacct
    18241 tcgccaccgc gtgggcgaaa cgatgcatcc cttcatcgat atccgcatct tccaccacca
    18301 gcgacggcgc aaaacgcatc acatcaggcc cggcattcag caccattacg ccttcctctg
    18361 caccggcata caggaaatca cgcgcccgac ctttgtactg tggtttcagc tccgcgccaa
    18421 ttaacagccc cataccgcga atatcgctaa acacatcgta ctgctgatcg atcttctgca
    18481 aatggtcaac aaaacgctgg cgtttcgcct gaatgccttc cagcacttca ggggtattga
    18541 tgatatcaaa cgccgcgccc gctactgcac aggccagagg attaccgccg taggtggaac
    18601 cgtgagaacc aggatgaaat gcagaagcaa tttccgccgt ggtcagcatg gcgctaatcg
    18661 ggaagccgcc gcctaacgct ttcgcagagg tcagaatatc cggcgtcaca ccgtagtgca
    18721 tgtaagcaaa caaatcgcca gtccgcccca tcccgcactg cacttcatca aacaccaata
    18781 atgcctgatg ttgatcgcac agctcgcgca agccctgcaa aaactctggc gtcgctgccg
    18841 tcacaccgcc ctcgccctgg atcggctcaa ccaccaccgc acaggtatgg tcatccatca
    18901 ccgctttcac tgcgtggaga tcgttaaaag gcacgtggat gatgtctgcc ggttttggcc
    18961 caaagccgtc ggaatatttt ggctgcccac ccaccgaaac ggtaaacagc gagcgaccat
    19021 gaaaagcgtt atggaaggca ataattttgg ttttgaacgg gctatgacgc acacaggcgt
    19081 aatggcgtgc cagtttaaag gcggtttcgt tagcttccgt gccggagttc ataaacacca
    19141 cgcgttcggc aaaagttgcc tcaatcagtt tacggccaag acgcagcgcc ggctcattgg
    19201 tgaaaacgtt actaatatgc cacagagttt cgccctgggt ttttaacgcg ttcaccagcg
    19261 caggatggca atggcccaac gccgtaactg caatgccacc cgcgaaatcg acatactcct
    19321 tgccctgttg atcccagatt cggctgccct gaccttttac cggaataaac tctgccggag
    19381 cataaatcgg cagaatcact tcatcgaaag tcgcgcgtgt aattgctgtt tgttcaattg
    19441 ccatctcatg atcaccctgt
//
LOCUS       DAZGHF010000088        19077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGHF010000088 DAZGHF010000000
VERSION     DAZGHF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.088"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(91..2757)
                     /gene="cas3"
                     /locus_tag="ACHK3A_003264"
     CDS             complement(91..2757)
                     /gene="cas3"
                     /locus_tag="ACHK3A_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFO8741311.1"
                     /translation="MEPFKYICHYWGKSSKSLTKGNDIHLLIYHCLDVAAVADCWWDQ
                     SVVLQNAFCRNEMLSKQKVKAWLLFFIALHDIGKFDIRFQYKSAESWLKLNPATPSLN
                     GPSTQMCRKFNHGAAGLYWFNQDSLSEQSPGDFFSFFDAAPHPYESWFPWVEAVTGHH
                     GFILHSQDQDKSRWEMPASLASYAAQDKQAREEWISVLEALFLTPAGLSINDIPPDCS
                     SLLAGFCSLADWLGSWTTTNTFLFKEDAPSNINALRTYFQDRQQDASRVLELSGLVSN
                     KRCYEGVHALLDNGYQPRQLQVLVDALPVAPGLTVIEAPTGSGKTETALAYAWKLIDQ
                     QLADSVIFALPTQATANAMLSRMEANASRLFTSPNLILAHGNSRFNHLFQSIKSRAFT
                     EQGQEEAWVQCCQWLSQSNKKVFLGQIGVCTIDQVLISVLPVKHRFIRGLGIGRSVLI
                     VDEVHAYDTYMNGLLEAVLKAQADVGGSVILLSATLPMKQKQKLLDTYGLHTDPEENN
                     SAYPLINWRGVNGAQRFDLLAHSEQLPPRFSIRPEPIYLADMLPDLRMLERMIVAANA
                     GAQVCLICNLVDVAQLCHQRLKELNNTQVDIDLFHARFTLNDRREKENRVISNFGKNG
                     KRNVGRILVATQVVEQSLDVDFDWLITQHCPADLLFQRLGRLHRHHRKYRPAGFEVPL
                     ATVLLPDGEGYGRHEHIYSNVRVMWRTQQHIEELNGASLFFPDAYRQWLDSIYDDAEM
                     DEPEWVIKGMDKFESAECEKRFKARKVLQWAEEYSLQDNDETILAVTRDGEMSLPLLP
                     YLQTSSGKQLLDGQVYEDLSHEQQYEALALNRVNVPFTWKRNFSEVVDEDGLLWLEGK
                     QNQDGWIWQGNNIVITYTRDEGMTRVIPANPK"
     gene            complement(3116..3850)
                     /gene="cysH"
                     /locus_tag="ACHK3A_003265"
     CDS             complement(3116..3850)
                     /gene="cysH"
                     /locus_tag="ACHK3A_003265"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO8741312.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(3924..5636)
                     /gene="cysI"
                     /locus_tag="ACHK3A_003266"
     CDS             complement(3924..5636)
                     /gene="cysI"
                     /locus_tag="ACHK3A_003266"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO8741313.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(5636..7435)
                     /gene="cysJ"
                     /locus_tag="ACHK3A_003267"
     CDS             complement(5636..7435)
                     /gene="cysJ"
                     /locus_tag="ACHK3A_003267"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO8741314.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVDGKTLTLSEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEDGEVRVFIEHNDNFRLPAN
                     PETSVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            7754..8116
                     /gene="queD"
                     /locus_tag="ACHK3A_003268"
     CDS             7754..8116
                     /gene="queD"
                     /locus_tag="ACHK3A_003268"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO8741315.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHYYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            8194..9465
                     /gene="ygcN"
                     /locus_tag="ACHK3A_003269"
     CDS             8194..9465
                     /gene="ygcN"
                     /locus_tag="ACHK3A_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8741316.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGTQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            9456..9716
                     /gene="ygcO"
                     /locus_tag="ACHK3A_003270"
     CDS             9456..9716
                     /gene="ygcO"
                     /locus_tag="ACHK3A_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO8741317.1"
                     /translation="MSVTRNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDEKTLQQWRYPPSGFGITYRFG"
     gene            9733..10308
                     /gene="ygcP"
                     /locus_tag="ACHK3A_003271"
     CDS             9733..10308
                     /gene="ygcP"
                     /locus_tag="ACHK3A_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO8741318.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(10455..11315)
                     /gene="ygcQ"
                     /locus_tag="ACHK3A_003272"
     CDS             complement(10455..11315)
                     /gene="ygcQ"
                     /locus_tag="ACHK3A_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO8741319.1"
                     /translation="MNIAIVTINQEHAAMAGWLAAQDFSGSTLTHWQIEPQPMVAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELSIRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAEINTMLPSGMLQLNIVPGALPDWLISIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLVEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKAVLEA
                     LVTTIHADCQ"
     gene            complement(11312..12091)
                     /gene="ygcR"
                     /locus_tag="ACHK3A_003273"
     CDS             complement(11312..12091)
                     /gene="ygcR"
                     /locus_tag="ACHK3A_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO8741320.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNIGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEDAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEITRETVAAEAPA
                     IQCLQLARPEQRRRAALIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(12069..13406)
                     /gene="ygcS"
                     /locus_tag="ACHK3A_003274"
     CDS             complement(12069..13406)
                     /gene="ygcS"
                     /locus_tag="ACHK3A_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8741321.1"
                     /translation="MNTSPVRMDDLPLNRFHCRIAALTFGAHLTDGYVLGVIGYAIIQ
                     LTPAMQLTPFMAGMIGGSALLGLFIGSLVLGWISDHIGRQKIFTFSFLLITLASFLQF
                     FATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFSPRRHRGVLLGAFSVVWTVGYVLAS
                     IAGHHFISESPEAWRWLLASAALPALLITLLRWGTPESPRWLLRQGRFAEAHAIVHRY
                     FGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFNSVFFVCLVIPWFVIYTWLPTIAQT
                     IGLEDALTASLMLNALLIVGALLGLVLTHLLAHRRFLLGSFLLLAATLVVMACLPSGS
                     SLTLLLFVLFSTTISAVSNLVGILPAESFPTDIRSLGVGFATAMSRLGAAVSTGLLPW
                     VLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKALPLVAAGNVGGANEHSVSV"
     gene            complement(13499..14953)
                     /gene="ygcU"
                     /locus_tag="ACHK3A_003275"
     CDS             complement(13499..14953)
                     /gene="ygcU"
                     /locus_tag="ACHK3A_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO8741322.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQVTAQCGVPLEVLENTLREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNHLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(15023..15808)
                     /gene="ygcW"
                     /locus_tag="ACHK3A_003276"
     CDS             complement(15023..15808)
                     /gene="ygcW"
                     /locus_tag="ACHK3A_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8741323.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGKTKEMIEKQGVEMDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            16127..17404
                     /gene="yqcE"
                     /locus_tag="ACHK3A_003277"
     CDS             16127..17404
                     /gene="yqcE"
                     /locus_tag="ACHK3A_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO8741324.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSTNNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            17431..18909
                     /gene="ygcE"
                     /locus_tag="ACHK3A_003278"
     CDS             17431..18909
                     /gene="ygcE"
                     /locus_tag="ACHK3A_003278"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO8741325.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWTSLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYDHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTILGHITPQAALATHFPAGLSVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMIRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         4623 a         4950 c         4701 g         4803 t
ORIGIN      
        1 agaagatgta cattgtgcac tttccctact taagtaggga taaaccgtta ttggtcttgt
       61 tatcgtcatt gataccaatc attcccgaag ttatttggga tttgcaggga tgactctggt
      121 catcccttca tcccgagtgt aggtaataac aatattatta ccctgccaga tccatccatc
      181 ctgattctgt ttcccttcca gccaaagtaa cccatcttca tctactactt cagaaaaatt
      241 acgtttccag gtgaagggta cattgacgcg attaagtgct aacgcctcat actgctgttc
      301 atgacttagg tcctcgtaga cctggccatc gagcagttgt ttacctgaag acgtttgtaa
      361 ataaggcaat aatggcaggc tcatttcccc atccctcgtt accgcaagaa ttgtttcatc
      421 gttatcctgc aagctatatt cttcagccca ctgcagaacc ttgcgagcct tgaacctctt
      481 ttcacactca gcgctttcaa acttatccat gcctttgatg acccattctg gctcatccat
      541 ttcagcatca tcgtaaatgc tatccagcca ttgccggtag gcatcgggga aaaataagga
      601 tgctccatta agttcctcaa tatgttgctg cgtccgccac atgactctaa cgttgctata
      661 aatatgctca tgtcgtccgt aaccttcgcc atcaggcagc aaaacggtgg caagaggaac
      721 ctcaaaacca gcgggacgat atttgcgatg atggcgatgt aaacggccca atcgttggaa
      781 aagtaaatct gcggggcaat gctgagtaat taaccaatca aaatcaacgt cgagtgattg
      841 ttccacgacc tgggtggcga caagtatccg tccaacattt cgcttcccat ttttgccgaa
      901 attgctaata actcgattct ctttttcacg acgatcgttc agcgtaaagc gcgcatgaaa
      961 caaatctata tctacttgcg tgttatttag ttcctttaac cgttggtggc atagctgtgc
     1021 aacatcaacc aaattgcaga taagacagac ttgtgcaccc gcatttgccg ctacgatcat
     1081 tcgctctaac atcctaaggt caggtaacat gtcagctaaa taaataggtt ctggccgaat
     1141 cgaaaagcgg ggcgggagtt gttctgaatg agctaacaga tcaaaacgtt gtgcaccatt
     1201 cacacctcgc cagttaatga gtggatatgc ggagttattt tcctctggat ctgtatgcag
     1261 accataagta tccagaagtt tctgtttttg tttcattggt agagttgcgg aaagaagaat
     1321 aacactcccc cccacatcag cctgagcctt gagcacagcc tccagcaagc cgttcatata
     1381 cgtgtcgtaa gcatgaactt catcaacaat taaaacactt cgaccaattc ccaaaccacg
     1441 gataaagcgg tgtttaactg gcaataccga tatcaacacc tgatcaatcg tgcaaacgcc
     1501 gatttgccca agaaacactt tcttattgct ttgtgacaac cactgacaac actgaaccca
     1561 tgcttcttct tgcccctgct cagtaaatgc gcgtgatttt attgattgaa aaaggtggtt
     1621 aaaacgtgaa ttaccatgag caagaataag atttggggag gtaaataaac ggctcgcgtt
     1681 cgcttccatt ctgctgagca ttgcatttgc agtagcttgt gtagggaggg caaaaataac
     1741 actatccgca agttgttggt caataagttt ccaggcatag gccagcgcag tttcagtttt
     1801 acctgaaccc gtaggtgcct ctattaccgt cagcccgggc gctactggaa gagcatcaac
     1861 taacacttgt aattgtctgg gttgatagcc attgtccagt agagcatgaa caccttcata
     1921 acatcgctta tttgatacaa ggccgctcaa ctccaatacc cggctcgcat cctgctgtcg
     1981 gtcctggaaa tacgttctta gagcatttat gttggaaggt gcatcctctt taaacagaaa
     2041 ggtattcgtt gtagtccagg agcctaacca gtcagcaagc gagcaaaaac ctgctaacag
     2101 tgatgaacaa tcaggtggta tatcgtttat agataacccg gctggcgtta aaaataatgc
     2161 ttcgagtaca gatatccact cctcacgagc ctgtttatct tgcgcagcat aagatgccag
     2221 agaagctggc atttcccaac gcgacttatc ttgatcctgg gaatgtaata taaaaccatg
     2281 atgtcctgta acagcctcta cccatggaaa ccaggactca taaggatgag gagcggcatc
     2341 aaaaaaactg aaaaaatccc cgggagattg ctctgaaagt gaatcctggt taaaccaata
     2401 cagaccggct gcaccatgat taaatttacg gcacatttgt gttgatggac cattaagtga
     2461 tggcgttgca ggatttaatt tcagccaact ttctgctgat ttatattgga atcgtatatc
     2521 aaactttccg atatcatgaa gggcaatgaa aaataacagc caggctttca ccttctgttt
     2581 tgatagcatt tcatttcggc aaaaagcatt ttgtagtacg actgattgat cccaccagca
     2641 atctgcaaca gctgcaacat ccaggcaatg ataaatcaac agatgaatat catttccttt
     2701 cgtcaggctt tttgaggatt tcccccagta atgacatata tatttaaaag gttccattaa
     2761 tagcctccct gtttttttag tattatcgat gatatcagtg gactattttt cgatgctgct
     2821 cataaatatc gaattacagt gatatacaca tgtcttgcct ccctgcaaag aagagactaa
     2881 cattcgaatt gcttaggttc gaacgctggc ctcaggttga tagaaatatc gcctggggct
     2941 tttgtccatc tggaacctcg cgaatgctta acgccagaca gcctcaagca cccaactcca
     3001 ttctatacct gttaattctc cctgcgatgt tcacctttaa atgccggaat gctgctcgca
     3061 aaataaaaac gcccccatca tttctgacag aggcgtttaa tttatccggc aatatttacc
     3121 cttcgtgtaa cccacattcc ctttttaagc caaagaaacg cgtttcttct tccgccatgc
     3181 cgggttccca tttacgggtt gtatgggtat caccgaccga taaatatcct tcgtcccata
     3241 atgggtgata tttcaggcca tgtttttgca ggtactgata aatagttcgg ttatcccagt
     3301 cgataatcgg cagcacttta aatacgccac gctgaattgc cagcaccggt aaattggcgc
     3361 ggctgccgga ttgttcacgg cgcaggccag caaaccaggt ttgcgcattc agttctttca
     3421 gcgcccggtt catcggttcg actttgttga tctcgttgta cttctcaatc ccctcaacac
     3481 cctgctccca cagtttaccg tagcgcgctt cctgccaggc ggcgctttcg gtagcgcggt
     3541 acactttcag gttgagcttg agtttgtccg ttaactcgtc aataaagcgg taggtttctg
     3601 ggaacagata gccggtatcg gtgaggatca ccggaatatc cgggcgaatc tgattcacca
     3661 gatgcaggct caccgccgcc tgaatgccaa agctggaaga aagtacatat tcaccgggca
     3721 gattatccag cgcccaggca acgcggcctt cagcgtccag tttttccagt tcggcgttag
     3781 tttccgccag cgccagaatg cgatctacct tcggcagttc gttcagggcg tttagatcga
     3841 gtttggacat agattcctca ctgtttgcct tgcctgatgc gacgctcacg cgtcttatca
     3901 ggcctacaag accgggctaa tggttaatcc cacaaatcgc gcgccggatc gagcaccggg
     3961 cgaatgatgc ccgcacgcac cgtaaagtcg ccgaagcctt cacccgcttc gcgctctttc
     4021 gcccagcgcc ctatcagttc atcaagcgac gccaggattt ccggctcggt gatgttttct
     4081 ttatacatcc gtgggatacg tgtcccaatg cggttgccgc caagatgcaa gttgtagcga
     4141 cctggcgctt tacccaccag gcccacttcc gccagcatcg cgcgaccaca accgttcggg
     4201 cagcctgtta cacgcatcac gatatgctca tcgctgacac catgtttcgc cattaaatta
     4261 tcgatgttgt cgataaaaga cggcaggaaa cgctcggctt cggccatcgc cagcgggcaa
     4321 gtcgggaatg acacgcaggc cattgagttt tcacgctgcg gcgtgacggc attcattaac
     4381 ccgctctctt tggcgatctt ctcgatcttc gctttctcgc tttccggcac accggcgatg
     4441 atcagattct ggttcgccgt aatgcggaaa tcgcctttgt ggatcttcgc gatctccagc
     4501 aggccggttt tcagcggacg cccaggataa tcaaggatgc gaccattttc gataaacagc
     4561 gtcagatgcc agttatcatc aatgccctta acccagccaa tacgatcgcc gcgtccggta
     4621 aactcatacg gacggatcgg ttcaaatttg atccccgcgc gacgctccac ttccgcttta
     4681 aacgtctcaa cccccacgcg ctccagcgtg tatttggttt tggcattttt acgatcggtt
     4741 cggttacccc agtcacgctg agttgtcacg acggcttccg ccactgccaa cgtatgctcc
     4801 agcggcagat agccaaactc actcgcggtg cgggcgtagg ttttcttgtt gccgtgttcg
     4861 atggaaagcc caccgcccac caacaggtta aagcccacca gcttgccgtt ttcggcgatt
     4921 gccacgaagt tcatgtcgtt ggcgtgcaga tcgatatcgt tctgcggcgg gatcactacc
     4981 gtggttttga acttacgcgg caggtaggtc tgaccaagga tcggctcttc atcagtagtg
     5041 gcgacttttt cctgatcgag ccagatctcc gcatacgcgc gggtacgagg cagcagatgt
     5101 tcagagattt tcttcgccca ttcatatgct tccgcgtgca gttgcgactc gtaagggttc
     5161 gaggtgcaga gtacgttacg gttcatgtcg ttagcagtcg ccagcgcatc aaggccgacc
     5221 gagtgcagca tctggtgcac cggtttgacg ttctttttca gaatgccgtg gaactgaaac
     5281 gtctggcggt tggtcaggcg aatgctgcca tagatggtgt tttcaccggc aaatttgtct
     5341 atcgcctgcc actgtttggt ggtgatcacg ccacccggca gacggcagcg caacagcatc
     5401 gcgtggcgcg gctccagctt ctgttcagca cgttcggcgc ggatgtcgcg gtcatcctgc
     5461 tgatacatgc cgtggaagcg gatcagcagg aagttgtcgc ccttaaagcc gccggtcaga
     5521 ccgtcgttta aatcttccgc aatggtgccg cgcaggtagt tgctttcaag cttcatgcgc
     5581 tcggcgtctg tcagttttcc ttcgaccact aaaggccctg gatgtttttc gctcattagt
     5641 agacatctcg ctgataacgg cgctctacgc gcagctcact taaaaattca tccgccgctt
     5701 cggtgtccat gccaccaaat tcggcaatca cttccagaag tgcctgctca acgtctttcg
     5761 ccatgcgatt agcgtcgccg cagacataaa tgtgggcacc gtcattgatc cagcgccaca
     5821 gctccgcgcc ctgttcgcgc agtttgtctt gtacgtaaat tttttctttt tgatcgcgcg
     5881 accaggcaag atcgatacgc gtcagcacgc cctctttgac gtagcgctgc cattccacct
     5941 ggtagaggaa atcttccgta aagtgcgggt tgccaaagaa cagccagttt ttccccggcg
     6001 cctcgtcggc ggcgcgttgc tgcataaagg cgcggaacgg cgcgatgccg gtgcctgggc
     6061 caatcataat caccgacgtt tccggattgg ctggcaggcg gaagttgtcg ttatgttcga
     6121 taaagacgcg gacttcacca tcttcttcca cacgatctgc gaggaagctg gaggcaccac
     6181 cggcacgggc gcggccttcc acgtcgtaac gcaccacacc aacggtaacg tgcacttcgt
     6241 tctcgacttc cgcctgcgag gaggcgatgg aatacaggcg cggggtcagc gggcgcagca
     6301 ggttaatcag cgcttcggca tccagttgcg ccggagagaa acgcaccatg tcaacaatcg
     6361 gcgtggtcgc ggcgtaatgc tgtaacttcg ctttatcgcc caccagcggc agcagcgttt
     6421 cgctgcgggt aagcgtggcg tagttctcaa caatattcgc ggtgttgacg gtcagttcaa
     6481 agtgccactg tagcgcttcg ctcagggtca gcgttttgcc gtcaacggtg acaggttcat
     6541 cgcctttcag ccacagcagt tcgacaagtt ctttcaccag tgccggatcg ttctgatacc
     6601 agacgcccag cgcgtcaccc ggctggtaac gcaggccaga gtcacctaag tcaatttcga
     6661 tatggcgaac gtctttttca gagttacgcc cggtaatttt ctggttaaca gagaggctcg
     6721 ccaccagcgg cgcgtctttg ctgtacgggc tggtgtggat ttcatttacc gcgccagtag
     6781 cgacggattg cgaaggtgcc gcgacaggcg cacgcgattt aagcgcatca accacgcggg
     6841 cgcgccactc gatggcagca gcctggtatt caacgtcggc atcgacacgg tcgagcaggc
     6901 gttcaccgcc cagttccgcc agcttactgt caaaatcttt cccggactgg cagaaaaatt
     6961 cataagaact atcgccgagg ctaaacacgg caaaagcggt attttccagc ttcggcgctt
     7021 ttttggagaa caggaactta tgcagcgcga cggcttcttc cggcggctcc ccttcccctt
     7081 gcgttgacgt cactacgatg agcagttttt cgctggcgat ttgtttgaat ttatagtcgc
     7141 ccgcgttcac cagcttaacg ttcagttttg ctgctaataa atcatcgcgt aatgcttcag
     7201 caacccggcg cgcattgccg gtttgcgagg cggagataat agttataccc ggcatttctg
     7261 cggctggcgc tggcgtcgct gcaagcgcag caggctgctg attgagtacg ccccagaaat
     7321 agccagaaac ccaggcaagc tgggtaggag ttaaatcggt cgtggccgcc tgaaggcgtg
     7381 ccagttgctc cgggttcaac ggaagcaacg cggaaggtgg gacctgtgtc gtcatgcgtc
     7441 gttatgttcc agtaagcaaa gctgtttctg cgccctgtca gcgcccataa aacagaagag
     7501 gaggtaaggt taacggggtg aacggtgtgg attaaagacg ggatagcgat aactaataac
     7561 caaatcgact aacctgtttt agcaatagtc tttaacaaca aaacagatta accaacccaa
     7621 tgaaaaacaa atgaatttag ccaatcatta agataaatca gcgattttgc gcaacaaagt
     7681 cgttttagat aatgcgaaaa aacagccttt ccggtactct acggcggttt tatcgtcctg
     7741 tagagaaatt atgatgtcca ccacgttatt taaagatttc accttcgaag ccgctcaccg
     7801 cttaccacac gtcccggaag ggcataaatg tggtcgcctg cacgggcatt cctttatggt
     7861 gcgactggaa attaccgggg aagtcgatcc gcatacgggc tggattatcg atttcgctga
     7921 actgaaagcg gcgtttaagc caacctacga gcgcctcgat cactattatc tcaatgatat
     7981 tccaggtctg gaaaacccga ccagcgaggt tttagcaaaa tggatttggg atcaagttaa
     8041 acccgttgtg ccgctgttaa gtgcggtgat ggtaaaagaa acctgcaccg caggttgcat
     8101 ctatcgcggc gaatgataag agtgtgtcgg cggtcaattt cccttaagta acgctatgtt
     8161 agggtgttgt gttctggata tctggggcat gacatggaag acgactgcga cattattatt
     8221 attggtgccg gtattgcagg caccgcttgc gcgttacgct gcgcgcgagc gggtttatcc
     8281 gttttgttac tggaacgcgc tgaaatcccc ggcagcaaaa atctttccgg cgggcggtta
     8341 tatacccatg cactcgcgga actcctccct cagtttcacc tgaccgcgcc tcttgaacga
     8401 cgcatcactc acgaaagcct ttccctgtta acgccggatg gcgcaacgac gttttccagc
     8461 ttacagcccg gcggtgaatc ctggagtgta ttacgtgcac ggttcgatcc gtggctggtt
     8521 gccgaagccg aaaaagaagg tgtcgaatgc atccccggtg cgacggtgga tgcgctgtat
     8581 gaagaaaacg gcagggtgtg tggtgtcatt tgtggtgacg atattctccg cgcccgttat
     8641 gtggtgctgg cagaaggtgc caacagcgtc ctggctgaac gtcacgggtt agtgactcgt
     8701 cctgctggcg aagcgatggc gttggggatc aaagaagtgc tgtcgctgga aacatccgct
     8761 attgaagaac gttttcatct ggagaataac gaaggcgcag cgttgctgtt cagcggcggg
     8821 atctgtgatg acttacccgg cggcgcattt ctttatacta atcaacaaac gctctcgtta
     8881 gggattgttt gcccgctctc ttcccttacg caaagtcgtg ttccggcaag cgagctgctg
     8941 gctcgcttta aaacgcatcc ggcagtgcgc ccgcttatca aaaacacgga atcactggag
     9001 tatggtgcgc atctggtgcc agaaggtggc ttgcacagta tgccggtgca atacgccggt
     9061 aacggctggc tgctgctggg cgatgcgttg cgcagttgcg tcaataccgg aatttccgtg
     9121 cgcggcatgg atatggcgct gactggcacg caggcggcgg cacaaacgct gataagcgcc
     9181 tgccagcacc gcgagccgca aaatctgttt gcgctttatc atcacaacgt agagcgcagc
     9241 ctgctatggg atgttctaca acgttatcag catgttccgg cgcttttgca acgccctgga
     9301 tggtaccgtg cgtggcctgc gttaatgcag gatatttccc gcgatttatg ggatcagggt
     9361 gataaacctg ttccaccgct gcgccagtta ttctggcgtc atttacgtcg tcacggcctg
     9421 tggcatctgg cgggcgatgt tatcaggagt ctgcgatgtc tgtaacccgt aatctctggc
     9481 gcgttgccga tgcgccgcac attgtaccgg ctgactccgt tgagcgtcag acggtacaac
     9541 ggttgattaa cgcttgtccg gctggtcttt tttcgctcac accggaaggt gacttacgcg
     9601 ttgattatcg cggctgcctg gagtgtggca cctgccgttt gctgtgcgat gaaaaaacac
     9661 tacaacagtg gcgctatccg ccttccggat tcggcatcac ctaccgtttt ggataaataa
     9721 taaggataat ttatgcccct cttacacctg ctccgccaga atccggtgat tgctgccgtt
     9781 aaagataatg ccagcctgca actggcaatc gactctgaat gccagtttat ttccgtgttg
     9841 tacggcaata tctgcaccat tagtcaaatc gttaaaaaga ttaaaaatgc cggaaaatat
     9901 gcttttattc atgttgattt gctggaaggc gcgtcaaata aagaagtggt gattcagttt
     9961 ttgaaactgg tgactgaggc ggacggcatt atcagtacca aagcctcaat gttgaaagcg
    10021 gcaagagcgg aaggtttttt ctgtattcat cgcctgttta ttgttgattc gatttcgttt
    10081 cacaacattg ataagcaagt tgcgcaatcg aatccggatt gtattgagat cctgccaggc
    10141 tgtatgccta aagtgctggg ctgggtgacg gagaaaatcc gccaaccgct gattgccggc
    10201 gggctggtgt gcgatgaaga agatgcgcgt aatgcgatta acgcgggtgt tgtggcgctt
    10261 tccaccacga ataccggggt ctggacgtta gcgaaaaaat tgctttgacg ggatactcgt
    10321 aacgagttga atttgtgtga tttgtaggct gcacgccaca tccgacattc agcgcctgat
    10381 acgacgcttg acgcgtctta tcaggcttac aagtccccag cccagaaccg taggcaggat
    10441 catccggcag cacgttattg gcaatctgcg tgaatggttg tcaccagtgc ctccagcacc
    10501 gctttccagt catcaaccac gccaacatct gcctgtgaaa ataccgccgc gctggcgtca
    10561 tgattaatcg ccaccacaaa cttgctattg cgtacgcccg ccattaacgc tgctgcgccg
    10621 gaagcgccaa caacaatgca cacctcaggc gccagcagat gtccggaaat accaatcact
    10681 ttttcagcat caacgccacc gttcatcact cgcgcccgac tgtagcccac ttcggctccc
    10741 agcttttcta ccagcatagc gatttcctgg ttatctgcct ctccgccctg ccccaccacc
    10801 agcacccgtc tggcttcagc taatggatcg cgggtaacat ttttcaggtt ttctatgcta
    10861 atcagccagt cgggaagcgc accggggaca atgttcagtt gcagcatacc agagggcaac
    10921 atggtgttta tctctgctcc cgcctgacgc gccagggaaa gacatagcgg gcgtttttct
    10981 gtttgcagcg tcgccgttag cgcatttccc cagtgggatt tgcgcacgct tacggttgga
    11041 atatctaacg aagtcacctg gcagatgctt gcaccatgta aacgccaggc cagccggatc
    11101 gacagttcat cgccaaacgc gcccggagga aaaaggacaa cctccgcagg cgttcgttgc
    11161 cactgttcca ccagcgcatc cagcacctgt tccgcaacca tcggctgtgg ttctatttgc
    11221 caatgcgtta gtgtgctgcc gctgaaatcc tgcgccgcca gccagcccgc cattgcggca
    11281 tgttcctgat ttattgtcac aattgcgata ttcatggctg catcctttgg cgtaaatagt
    11341 cctgccatag cttttgcgct ttttctgcta ccgtttgccc atcaatcagc gccgctctgc
    11401 gtcgttgctc tggccttgcc agttgcaggc actgtatcgc tggcgcttcc gcggcaacag
    11461 tttcgcgggt aatctccgct ttccccgctg ccattcgctg gcgcattccc ggtactggca
    11521 aggccacttc accacactga cgcacggcaa tcaccgcagg caagcgaacc cggcaacagc
    11581 gcaacccatg ttcagtacgc tgttcgaggg tgataaacgg cgcgtcgaga gtaaaacgct
    11641 ccacctgggt gaaacagggc cagcccagca tttccgccag caaaaatggc gtttgtccat
    11701 tctgcccttc gctgctttgg cagccggtaa tgatcagatc cagcggattc tgacgctgcc
    11761 attcggcaat atggcgagcg acaaattccg gcgcaaagcg cagatctgcc gccgtctcca
    11821 gcaatacagc gtcctcaaac ccaagagcca tgagatagcg cagccagtgc aacgcccgtt
    11881 catcccccat gctcaacgcg gttaaagaca tcggcgtacc gttttttctc tgcgccaaca
    11941 gcagcgcggc ggcagcctgt tcatcggcac cgagtaaact tcgcagaagc gaaacatccg
    12001 gtccgatatt accctgagcc gccgcctgcc actctttttc cgccagcatt ccggcatccg
    12061 gttcggcttt aaacgctaac agaatgttca ttcgcacctc ctacatttcc cgccgccacc
    12121 agcgggagtg ctttggtttc tggtgcccat agccaggtca caacaaaacc aaccaacaac
    12181 actgccgcca ggagcaataa ggtggcttgc attccccact gcgccagcac ccacggcagc
    12241 aggccagtac ttaccgccgc ccccaaccga ctcatggcgg tggcaaaacc gacgcccagc
    12301 gagcgaatgt cagtaggaaa actttccgca ggcaaaatgc ccaccagatt actgactgcc
    12361 gaaatggtgg tgctgaagag aacaaaaagc agcagcgtta atgaactgcc ggaaggcaaa
    12421 caggccatca cgaccagcgt tgccgccagc agcaaaaaac ttcccagtaa aaatctgcga
    12481 tgcgctaaca agtgggtcaa cactaaccct aataacgcgc caacaattaa caacgcatta
    12541 agcatcaggc tggcagtcag cgcatcttcc agaccaatag tttgggcgat ggtcggcagc
    12601 caggtataaa tcacaaacca tgggattacg aggcagacaa agaagacgct gttaaacgct
    12661 gtgcgccgcc agtagcgcga agagaacaag gttttgatgt gtttatgggt cgccgttgcc
    12721 acttcatcgc ccagtaaaac atggggacca aaatagcgat gcacgatagc gtgagcttct
    12781 gcaaaacgcc cctggcgcag tagccagcgt ggcgattctg gcgttcccca gcgtaataac
    12841 gtaatcaaca atgcgggcag agctgccgat gccagtagcc agcgccaggc ctccgggctt
    12901 tcgctaataa agtgatgtcc ggcaatactt gccagcacat agcctacggt ccacaccacg
    12961 ctgaatgcgc ccagcaaaac accgcgatgg cggcgcggag aaaattcagc cagcaaagtg
    13021 tgaccgactg agtaatcgcc tcccagacca atgccgatca aaatgcgcag tccaataaga
    13081 tgctctggcg tggtggcaaa aaattgtaag aacgaagcga gcgtaatcag caaaaagctg
    13141 aaggtgaaga ttttttgccg accaatatgg tcggagatcc accccaaaac caggctaccg
    13201 ataaacaggc caagtagcgc cgagccgccg atcattcccg ccataaacgg cgtcagttgc
    13261 atggcgggtg taagctgaat aatggcgtaa ccaatgacgc cgaggacata accgtcggtc
    13321 aggtgtgcgc cgaaagtgag cgcagcaatg cggcagtgaa aacggttaag tggtaaatca
    13381 tccattcgca ccggtgaagt gttcatcttt accccttcat tgtccggtaa taagaacggc
    13441 ttctcgcctg agaagccgaa aaggcaacgc ccgtcatcgc ggaacgctgc ctgacacatt
    13501 atttttcaat cggatagata gtacccgtat tcataatgcc gttaggatcg aactgctttt
    13561 tcagcccttc cagcaacgcc cacgcgctgc cgtgttccag tttgctccag tgaacgcgat
    13621 gtttaccgat accgtggtgg tgcaccatcg aaccaccgag gcgaatggtt tcttcgcaga
    13681 tgattttgtt gagcggattg tggtacttgt cgatttcctc ttccggctta cagtcaacga
    13741 cgttgtagtc gtagacgaag tacatgttgg tgccgttctg atagctatga gaggagtgac
    13801 cgcccagcat ggtgatgtcg tcggcatgcg ggaactcggt acgaatacgg ttaataacgc
    13861 tttcgtagat ttcgtggatg cagctccagc agccggacac ttcggtggta aagcccatgt
    13921 tgccggtttt gaggatctgc acacgttcag cggccacttt atccggcccc cagttcaggt
    13981 gattaaacca ggtttcgatc agcttgctgt ccactcgctg gcattgcggg taacgtgcca
    14041 cgatttcagc aatcccttcg cctgtcgcct tcgcaatgcg agggttacct tcagccataa
    14101 agatcagcac acatttacca tcggcaaaat gggtgaagtg ttgggtgcca tcttcagcgt
    14161 catacaaacg agcgatcgac ggacgatacc cttccaccat gatttcacgc aggatgttga
    14221 agccggtttt catgtcttcc agcacatagc catagaagag attgttttcc ggcgtaaatt
    14281 tgaagatttt cactgtgact tcagtgatat agcacaatgc accttcgttg ccgatgataa
    14341 tgtgacgaat gtccgggcct gccgcgcggc gtggtacgtt tttaatacgt gtgacggtgc
    14401 catcagccaa tactgcttcc agaccaacga ccatatcttc gattgcgccg tagagtgtgg
    14461 agaactgccc gatactgcgg gttgctacca ggccgcccat ctgcgccagc ggttttgatt
    14521 gcggtgaatg ccccgtggtg taaccttttt cacgcaacgt gttttccagc acctccagcg
    14581 gaacaccaca ttgcgccgtg acctgcatat tctcaatatc aatattaatg atttgattca
    14641 tggcagagcc gtcgagcacc accgagtttt ccacaacggt ttccagtcca ccctcggtag
    14701 cggaagcacc ggtacgcggc acaccgttaa ttttgtgtgc attcataaaa ttcagtacac
    14761 gagagacttg ttctgtggaa ccgagtttta cgaccgctgc cggaatcggc aaagtataaa
    14821 tgccatgaat atccggaaat ttgcgaaaac ggtcaatact gtttttcttt aatactgttt
    14881 catcggtaat tacgcgatct gcaccaacaa tttcctttag ctggtcgaca atcgctgcgc
    14941 gagataaaga catagtaatt ccttcctgat aataaaaatg gaataatcat taataagaga
    15001 aaacagaaac caccgttatg gattagcgca ctaaataacc gccatcgacc actaataaat
    15061 gcccgttgac ataattcgat gcctgacttg cgagaaacac ggctgcgccc attaaatcct
    15121 gagtatcgcc ccaacggttt gccggaatat gatcaagaac gcgctgattg gtttctggat
    15181 tactgcgtgt cgccagcgta atatctgttg cataatagcc aggggcaata ccatttacct
    15241 gaatattata ttgacctagt tcatcacaat aagctttggt gaacccggca agagcatgtt
    15301 tagtggcaga atatgcaggt gaccattgtc cacctaagta agagaacaat gaacagatat
    15361 taataatttt accgcttttc tgcgggatca taatttttgc cgcttcatag cttaactcga
    15421 atgcggcggt caggttcaca tcaatcatcg ggtcccagtc ggcacgaccg aaatccagca
    15481 ccttattcag cttacaaata ccggcattgt taaccagaat atcaactgtt ccgaaacgct
    15541 cacagcaagc agcgataatc ttctgcggcg cgccttctgc ggtgatatcc acctgcatga
    15601 agtccatctc aacaccctgt ttttcaatca tttcctttgt tttgccgtta tctttaacga
    15661 agcttggaat aaagatattt gcaccagctt tggccagcgc catggcaaat gcctggccta
    15721 aaccgctatt cccgccggta acaattgcgg ttttaccttt cagggagaaa aaatccattg
    15781 agaacgcatt gagagattcg attgacatag ttagctccaa tttccgtaag gcaaaaaaaa
    15841 agaaaggtaa cttcccccgc gagagggaag ttacctttct cattatctct ggtaactgga
    15901 gataattttt atcataaggg cataatcgct aatgtgatcg taatcacagt gttatattcg
    15961 aattgtcaaa taattttaaa aagcagaagc gtgattactc tcacacaaca ccactgcttt
    16021 ccatgttatt tcttattgca attactagag acctgagaaa agtaatccac ctgaaagggc
    16081 ggcttacttt tctctttttt ttgtctatta caaatgggaa tacgatatgc aacacaactc
    16141 atatcgccgt tggataaccc tcgcgataat tagttttagc ggcggcgtta gtttcgatct
    16201 ggcttattta cgttatattt atcaaattcc catggcgaaa tttatgggat tcagcaatac
    16261 cgagataggt ttaataatga gtacctttgg tattgcggct attattcttt atgcccccag
    16321 cggcgttatt gccgataaat tttcacaccg caaaatgatt acttccgcga tgatcattac
    16381 cggattactg ggtctgttaa tggcaacata tccaccgctg tgggtaatgc tctgtattca
    16441 ggtcgccttt gcgataacga cgattttaat gctgtggtcg gtgtcgatta aagccgcctc
    16501 attgctgggc gatcatagtg agcaagggaa aattatgggc tggatggaag ggctgcgcgg
    16561 cgtcggtgta atgtcgctgg cggtgtttac catgtgggtc ttttctcgct ttgcgccgga
    16621 tgacagcgcc agcctgaaaa cagtcattat tatctacagt gtggtttaca tcttgttggg
    16681 gattctgtgc tggttttttg ttagcgataa caacaacctg cgcagtacca ataacgaaga
    16741 aaaacagtca ttccagctta gcgatatcct ggccgttttg cgtatcagta ccacctggta
    16801 ttgcagcatg gtgatttttg gtgtcttcac catctacgcc attctgagtt actccaccaa
    16861 ctatctgacc gaaatgtacg gcatgtcgct ggtggcggcg agctacatgg ggattgtgat
    16921 caacaaaatc ttccgcgcgc tgtgcggccc acttggcggc attatcacca cctacagtaa
    16981 agtgaaatcc cctacccgcg tgatccaaat cctttccgta ctcggcctgc tggcgttaac
    17041 tgccctgctc gtcacgaact ctaacccgca atcggtcgcg atggggattg gcctgatttt
    17101 actgctggga ttcacctgtt acgcctcacg cgggctgtac tgggcctgcc caggcgaagc
    17161 gagaacaccg tcttacatta tgggcaccac ggtaggtatt tgttcggtga ttggattcct
    17221 gccggatgtc ttcgtttacc caattatcgg ccactggcaa gacaccctgc ccgcagcaga
    17281 agcctaccgc aatatgtggc tgatgggcat ggcggcgctt ggcatggtga ttgtctttac
    17341 ctttttgctg ttccaaaaaa ttcgtactgc tgatagcgcc cccgcaatgg ctagcagcaa
    17401 gtaagccagg aaaattacag gagaaaaatc atgtcgaaaa aatacatcat agggattgat
    17461 ggcggaagtc agagcacaaa agtggtgatg tacgatctgg aaggtaatgt ggtttgcgaa
    17521 ggtaaaggct tattgcagcc gatgcatacg ccagatgccg atactgccga acatcctgac
    17581 gacgatttat ggacatcgtt atgctttgcc ggtcacgatt tgatgagcca gtttgccgga
    17641 aataaagaag atattgtcgg tattggtctg ggctccatcc gttgctgtcg tgcgttattg
    17701 aaagccgatg gcacgcccgc tgcgccattg attagctggc aggatgcacg tgtaacacgt
    17761 ccctacgatc ataccaatcc agatgtggcc tatgtcacct ctttttcggg ttatctgacg
    17821 catcgcttaa ccggcgagtt taaagataat atcgccaact attttggtca gtggccggtg
    17881 gattataaga gctggacatg gagtgaagat gctgcggtaa tggataagtt taatatcccc
    17941 cgtcatatgc tgtttgatgt gcaaatgcct ggcaccattc ttgggcatat cacaccacaa
    18001 gctgcgctgg cgacacattt cccggctggg ctgtcggttg tttgtaccac cagtgataaa
    18061 ccggtagaag ctctgggagc cgggttactg gatgatgaaa ctgcggtaat ttctcttggc
    18121 acttacatcg cattgatgat gaacggcaaa gccttgccga aagatccggt ggcgtactgg
    18181 ccgattatgt cttctattcc gcaaacattg ctgtatgaag gttacggtat tcgcaaaggc
    18241 atgtggacgg tgagctggct gcgcgacatg ttaggcgagt cgttaattca ggatgccagg
    18301 gcgcaggatc tttcaccgga agatttactc aacaagaaag cctctagtgt gccacctggc
    18361 tgtaatgggc tgatgacggt gctggactgg ctgaccaatc cgtgggaacc gtacaaacgc
    18421 gggattatga tcggctttga ttccagtatg gattacgcat ggatatatcg ttcgatactg
    18481 gaaagcgtgg cgctgacgct gaagaacaat tacgacaata tgtgtaatga aatgaatcac
    18541 tttgcgaagc atgtaatcat tactggcggc ggttcgaaca gcgatctgtt tatgcagatt
    18601 tttgccgacg tgttcaacct tccggcacga cgtaacgcca ttaacggttg tgcaagtctg
    18661 ggggcagcga ttaataccgc ggtaggtctg gggctatacc cggattacgc aacggctgtc
    18721 gacaaaatga ttcgcgtgaa agatatcttt ataccgattg agagcaatgc caaacgctac
    18781 gacgcgatga ataaaggcat tttcaaagac ctaaccaaac atactgatgt gatcctgaaa
    18841 aaatcgtatg aagtgatgca tggggaattg gggaatgcgg attcgatcca gagctggtcg
    18901 aatgcgtaag tgaggaaggc cgggcgggaa actgcccggc ctgaacatac ctgaatggtt
    18961 atccccgctg acgcggggaa cataagttcg acaacttaaa aaatgataac aagtccggtt
    19021 tatccccgct gatgcaggga acataataaa aactttaact agttcggttt atccctg
//
LOCUS       DAZGHF010000089        18892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGHF010000089 DAZGHF010000000
VERSION     DAZGHF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.089"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            50..1003
                     /gene="ybhD"
                     /locus_tag="ACHK3A_003279"
     CDS             50..1003
                     /gene="ybhD"
                     /locus_tag="ACHK3A_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8741326.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1044..2039)
                     /gene="pgl"
                     /locus_tag="ACHK3A_003280"
     CDS             complement(1044..2039)
                     /gene="pgl"
                     /locus_tag="ACHK3A_003280"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO8741327.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSEDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2194..3012
                     /gene="ybhA"
                     /locus_tag="ACHK3A_003281"
     CDS             2194..3012
                     /gene="ybhA"
                     /locus_tag="ACHK3A_003281"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO8741328.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3013..4071)
                     /gene="modC"
                     /locus_tag="ACHK3A_003282"
     CDS             complement(3013..4071)
                     /gene="modC"
                     /locus_tag="ACHK3A_003282"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO8741329.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4074..4763)
                     /gene="modB"
                     /locus_tag="ACHK3A_003283"
     CDS             complement(4074..4763)
                     /gene="modB"
                     /locus_tag="ACHK3A_003283"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO8741330.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4763..5536)
                     /gene="modA"
                     /locus_tag="ACHK3A_003284"
     CDS             complement(4763..5536)
                     /gene="modA"
                     /locus_tag="ACHK3A_003284"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8741331.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5702..5851)
                     /gene="acrZ"
                     /locus_tag="ACHK3A_003285"
     CDS             complement(5702..5851)
                     /gene="acrZ"
                     /locus_tag="ACHK3A_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO8741332.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5980..6768
                     /gene="modE"
                     /locus_tag="ACHK3A_003286"
     CDS             5980..6768
                     /gene="modE"
                     /locus_tag="ACHK3A_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO8741333.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARNHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6836..8308
                     /gene="modF"
                     /locus_tag="ACHK3A_003287"
     CDS             6836..8308
                     /gene="modF"
                     /locus_tag="ACHK3A_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO8741334.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARAQAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            8569..9585
                     /gene="galE"
                     /locus_tag="ACHK3A_003288"
     CDS             8569..9585
                     /gene="galE"
                     /locus_tag="ACHK3A_003288"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO8741335.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9595..10641
                     /gene="galT"
                     /locus_tag="ACHK3A_003289"
     CDS             9595..10641
                     /gene="galT"
                     /locus_tag="ACHK3A_003289"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO8741336.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10645..11793
                     /gene="galK"
                     /locus_tag="ACHK3A_003290"
     CDS             10645..11793
                     /gene="galK"
                     /locus_tag="ACHK3A_003290"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO8741337.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDATIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11787..12827
                     /gene="galM"
                     /locus_tag="ACHK3A_003291"
     CDS             11787..12827
                     /gene="galM"
                     /locus_tag="ACHK3A_003291"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO8741338.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPF
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYTDWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            13030..13782
                     /gene="gpmA"
                     /locus_tag="ACHK3A_003292"
     CDS             13030..13782
                     /gene="gpmA"
                     /locus_tag="ACHK3A_003292"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO8741339.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13930..14982)
                     /gene="aroG"
                     /locus_tag="ACHK3A_003293"
     CDS             complement(13930..14982)
                     /gene="aroG"
                     /locus_tag="ACHK3A_003293"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO8741340.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            15298..15678
                     /gene="ybgS"
                     /locus_tag="ACHK3A_003294"
     CDS             15298..15678
                     /gene="ybgS"
                     /locus_tag="ACHK3A_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO8741341.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15792..16733
                     /gene="zitB"
                     /locus_tag="ACHK3A_003295"
     CDS             15792..16733
                     /gene="zitB"
                     /locus_tag="ACHK3A_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO8741342.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16730..17449)
                     /gene="pnuC"
                     /locus_tag="ACHK3A_003296"
     CDS             complement(16730..17449)
                     /gene="pnuC"
                     /locus_tag="ACHK3A_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO8741343.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQMFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17487..18530)
                     /gene="nadA"
                     /locus_tag="ACHK3A_003297"
     CDS             complement(17487..18530)
                     /gene="nadA"
                     /locus_tag="ACHK3A_003297"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO8741344.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCSVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPV
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18778..>18892)
                     /locus_tag="ACHK3A_003298"
     ncRNA           complement(18778..>18892)
                     /ncRNA_class="other"
                     /locus_tag="ACHK3A_003298"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         4738 a         4964 c         4720 g         4470 t
ORIGIN      
        1 tttaactgcc agtcacccaa aatcgtcgct tccattttcc ggagtaataa tgaagcatga
       61 attatcaagt atgaaggcat ttgtcatact ggcagagtcc agttcattta acaatgctgc
      121 taaattactc aatattacgc aacctgcatt aacgcgaaga ataaaaaaga tggaggaaga
      181 tttacacatt caactatttg agcgtacaac tcgcaaggtt acgttaacaa aagcaggaaa
      241 aaggttgctc ccggaggcgc gggaattaat aaaaaaattc gatgaaacgc tttttaatat
      301 tcgtgatatg aatgcttatc atcgtggtat ggtgacgtta gcgtgtattc cgaccgcagt
      361 attttatttt ttaccgctgg caatcggtaa atttaatgag ctgtatccca atattaaagt
      421 gcggattctg gaacaaggca cgaataattg catggaatcg gtgctgtgta acgaatcaga
      481 ctttggcata aacatgaaca acgtcacaaa ttcatccatt gattttaccc cactggtcaa
      541 cgagccgttc gtgctggcct gtcggcgtga ccatccattg gccaaaaagc agcttgtaga
      601 atggcaggag ttagtgggtt acaaaatgat tggcgtacgc tcctccagcg gcaaccgact
      661 attgatagaa caacagctag ccgacaagcc ctggaagctg gactggtttt acgaagtgcg
      721 tcatctttcg acgtcattag ggctggtgga ggcgggactg gggatttcgg cgcttcctgg
      781 tctggcaatg ccccatgcgc cctattcttc gattattggt atcccgctgg tggaaccggt
      841 tatacgtcgg acattgggga ttattcgccg caaagatgcc gtgctttctc cggcagctga
      901 acgctttttt gccttgctga ttaatttgtg gactgacgat aaagacaatt tgtggaccaa
      961 tatcgtcgaa cgccagagac atgcgttaca ggagataggt taatacaccg gcgcagaaga
     1021 gactgcgccg ggtaaatcag cggttagtgt gcgttaacca ccacccacat tggtccctgc
     1081 ccgaccgcat agcggccttt ttcatgcagt agcccctgct cgccaacaat ttcgtatacc
     1141 gagatgtggt gagatttttg cccggcggca atcagatact tgccgctgtg atcaacattg
     1201 aagccgcgcg gctgggtttc agttggctgg aagccttctt tactcaacac gctgccatct
     1261 tccgaaacgc tgaaaacagt aatcagactg gcggtacggt cgcaggcgta taaatgacga
     1321 ccatccgggg tgatatgaat atcagccgcc caacgggtgt cggagaagtt ttccggcatc
     1381 atatccagcg tctggacaca ttcgatattg ccatgcggat ctttcagttc ccagacatcc
     1441 actgagctgt ttaactcatt gacgcaatac gcatattgtt cgtttggatg gaataccata
     1501 tgacgcgggc cggccccttc aacggtagtc acttccgcag ggtcctgcgc caccagatga
     1561 ccatcctcgc tgacagtaaa caggcaaatg cgatcctgct ttaatgccgg aacccacagc
     1621 gtacggttgt ccggtgagat attggcggaa tggcaaccgt ccagcccctc gaccacatcg
     1681 acgacgccca ctggcaggcc atcttccaga cgcgttacgc tcacgttacc cgcattgtaa
     1741 gaaccgacaa agacaaactg cccctggtga tcggtggaaa tatgcgtcgg actacccggc
     1801 agcgcagact ctgcggcaaa ggtcagcgcg ccatcgtccg gggcgatacg atacgccagg
     1861 acgcgaaact cagggcgaac accaacatag agataacgtt tgtccgggct gaccaccatc
     1921 ggctgcacct gccccggcac gtcgacaacc tgtgtcagcg tcagtgcgcc ttcatgattc
     1981 aggttccaga cgtgaatttg ctggctctca gggctggcga tataaactgt ttgcttcatg
     2041 aatgctcctt tgcatttagc aactgactgt aacagctaaa attagtcgct tttggcggtg
     2101 aatgctgaat taaaacgcag aattttgtcc gatagtggta tcggtgtacc attcgcgcag
     2161 acgaaaattc ttaaccttaa tctggaatac gccatgacca cacgcgtgat tgctctcgac
     2221 ttagacggca ccttattgac cccgaaaaag accttgcttc catcatcgat agaagccctg
     2281 gcccgcgctc gcgaagcagg ctatcaatta atcatcgtca caggtcgcca tcacgtcgct
     2341 attcatcctt tttatcaggc gctggcgctg gatacacctg ctatttgctg taatggcacc
     2401 tatttgtatg attatcatgc aaaaacagtg ctggaagcgg acccaatgcc cgttaataaa
     2461 gccctgcaac tcattgagat gctgaatgaa caccacattc acggtctgat gtatgtcgat
     2521 gatgcaatgg tctatgagca cccgaccgga catgtcattc gcacgtctaa ctgggcgcaa
     2581 accctgccgc cggaacagcg tccgactttc acacaagtcg cttctctggc tgaaacggcg
     2641 caacaagtta acgccgtatg gaagttcgcc ctgacgcacg atgacctgcc gcaattgcag
     2701 cattttggta agcatgtcga acatgaactg ggactggagt gtgaatggtc ctggcacgat
     2761 caggttgata ttgcacgcgg cggcaacagc aaaggtaaac gtttgacgaa atgggttgag
     2821 gcgcaaggct ggtcgatgga aaacgtcgtg gccttcggcg ataacttcaa tgatattagt
     2881 atgctggaag ccgctggtac aggcgtggcg atgggcaacg ccgatgacgc agtaaaagcg
     2941 cgcgccaaca ttgtgattgg tgataacacc accgacagca ttgcccagtt catttatagc
     3001 cacctgattt aatcaggcgg ttatcgacac acttttaatt tgcgcataca accacaggcc
     3061 aggtttgatc gccagttcat ccctggccca cgggctgata cgcgcccaca gcgttttacc
     3121 gccgacttcc agctccactt ccacctggcc gttgtcgtca taactattaa ccacttttgc
     3181 ccgcaatacg ttacgaatgc tggtttgctg cggcggttgt aaaaccagcg agacatcgga
     3241 agcctgaatg cggatgcgta gcgcagcttg cagcggttcg tccagcttat tgacccacaa
     3301 atgctgatcg cccagcgcca gcgcggtcat cgcgtaatgc ggatgatgct caagcaccgt
     3361 cactttcaga atgctacttt gttgctcttt cggcagccac ggattcatca cgctactgcc
     3421 ccacacttcc tccagcgcgc caaaggcttt cacctgaccg ttttccagta ccatcactct
     3481 gtctgccaga tggaggatct catccagcga atggctgaca tacaacatcg gaatgttgat
     3541 ttcccgtgtc agccgttgca gataaggcaa cagttcgcgt ttacgcggaa tatccagtga
     3601 cgccagcggt tcatccagca acagcaattc cggtgctgtc agcaaagccc gaccaatcgc
     3661 cacgcgctgt ttttcacctc cggacaggct gcctggtaaa cggtcaagca acggttcaat
     3721 gcctaaaagc gccaccagct tatcgaactg atcgaccata cttttcgcca tgccgtagcg
     3781 cagattgcca cgcactttgt aatgcgggaa cagccgcgca tcctgaaaaa catagccaac
     3841 gcgacgcttt tccggcgtca ggcagatacc tttttcggca tcatttagta cccgcccatt
     3901 gaggacaatc cgcccttttt gcgggcgcgt cagtccactg atggcgttaa tcagcgaagt
     3961 ttttccggca ccggagacgc caaagatagc agtgatgcca ttggcgggca gcgtttcatt
     4021 aatggtcagg caatggttgc ccaacgtctg ggaaaaattc agttccagca tgattagcgc
     4081 cccgcccgtt cacggctgat tctggccagc cattctgaaa tcaacaggga gatcatcgcc
     4141 agtgcaatag aaataatgca cagtctcgcc gctccacttt ccccgccggg ggtctggatc
     4201 agggtataca tggcagaagg aatggttcgc gtttcaccag gaatgttcga cacaaaggtg
     4261 atggttgcac caaactcacc gagagaacga gcaaaagcca gtaccgtacc aacaataatg
     4321 cccggtaagg tcagcggtaa cgtgatagta aagaaaacgc gccagcgccc ggcacccagt
     4381 gttcttgcgg cctgttccag tttgacatca accccttcca gcgccagacg aattgcccgc
     4441 accatcagcg gaaacgacat gacggcggca gcgagaaccg cgccgcgcca gctaaaggcg
     4501 aaggtaatgc caaaccagtc atacagacgt tcaccgataa atccgcgccg tcccatcgaa
     4561 actaataata agtaaccgac gaccacgggc ggtaacacca gcggtagatg cagtacgctg
     4621 tcgagcagag ctttgcccgg aaacgtgcaa cgcaccagta accaggcaaa aaagatccca
     4681 aacggcaggc taaacagcac agccagggaa gaaactttca ggcttagtaa aaccgcctgc
     4741 cattctggat cggtcagtat cattacttgg ttgtaaatcc gtaacgttta aagatttccg
     4801 cagcctgcgg tcccttcaga taatcataaa atgctttcac cgtcgcattg ttatgccctt
     4861 ccacaacagc aaccggatat tccacttttt tatgcgaatc ttccgggaag gtggcaacca
     4921 cttttacccc tttgctggca actgcgtcag aaccgtaaac aatacccagc ggcgcttcat
     4981 tgcgttcgac caacgccagc gcgccacgaa catcttccgc cggagccagt ttcggggaga
     5041 gcgtatccca tgcgcccagt ttttgcagtg cttcttttgc ataaatgcca gcgggaacat
     5101 gttccggatc gccaaccgcc agacgaccgc cattcagcag cgaagtccag ttggttttgc
     5161 tgtcgatggt gaaatctttc tgctcgctgg ctttcggtgc tacaacgacc aggctattgc
     5221 cgagcagtgt ctggcgcgaa gccgtatcga tcgctttttt atcaaccgca taatccatcc
     5281 atttctgatc ggcagaaata aacagatccg caggcgcacc cgcttcaatc tgacgggcca
     5341 aagtagatga cgaagcgaaa gaagaaacca catccacgcc tttctctttt ttatactgcg
     5401 tagcaatgtc ctgcattgcg ttagtcagtg atgcggcggc gaacaccgtg attttccctt
     5461 catctgccag tgcattgcca gcaacagcga aagagagtgc cgccccggca aacaagttca
     5521 accatttacg agccattggt aaccccttaa tctaacgtta tgtaggcgac aatataacgg
     5581 ctaactcaga attttcccag cagttattgc taacctttgt gaggtagatg agaaaaaata
     5641 tcggcaggaa aagcaggagg ttgagaaaaa gaaatgcccg actaagcggg cattcaggga
     5701 ttcaatgatt ttgtccgggc tggtcttttt taccaacacc agaaaagatg ttgaatactt
     5761 caccaagacc gtaaatcaga cccaggatga tggccatcac gacaggtacc atgattacgg
     5821 cgaataccag actttttaat aactctaaca tggtcaactc cagatatagt catgagacta
     5881 ttctaaccgc taagcaaaga aaagcactcc ccttttgtgc ggtcagcttt gcgtggcgtt
     5941 cgttttccgt cacaataaga ctttttccag gatattgtta tgcaggccga aatccttctc
     6001 acccttaagc tccaacaaaa attattcgcc gacccgcgcc gcatttcgct actaaaacac
     6061 attgcgcttt ccggttccat tagccaggga gcgaaagatg ccggtatcag ctataaaagc
     6121 gcatgggatg ccattaacga gatgaatcag ttaagtgagc atattctggt tgagcgcgca
     6181 acaggcggta aaggtggagg cggcgcagta ctgacccgct atggtcagcg actgattcag
     6241 ctctatgact tactggcgca aatccagcaa aaagccttcg atgtgttaag tgacgatgac
     6301 gccctgccgc tgaacagcct gctggccgcg atctcacgtt tttcactgca aaccagcgcc
     6361 cgtaaccaat ggtttggcac catcaccgcc cgcaatcatg atgacgttca acagcatgtt
     6421 gatgtcttac tggctgacgg aaaaacacgc ctgaaagtcg caattaccgc acaaagcggc
     6481 gcgcgtctgg ggctggatga aggcaaagaa gtgttgatat tactaaaagc gccgtgggta
     6541 ggcattactc aggacgaagc ggtcgcacaa aacgccgaca accaattacc gggtattatt
     6601 agccatattg agcgcggcgc agagcagtgc gaagtattaa tggcgctacc cgacgggcaa
     6661 acactgtgcg ccacagtgcc ggtaaatgaa gcgacttctc ttcagcaagg acagaatgtc
     6721 acggcctact ttaatgccga cagcgtgatt atcgccacgc tgtgctaagc gtgttgacaa
     6781 tttgttatga aacacgtatc cctgtcagta atcgctgcac aaaatgggat ataaaatgtc
     6841 atcgttgcaa attttgcaag gcacgtttcg tcttagcgac acaaaaacgc tgcaattgcc
     6901 tcagctaacg ttaaacgcgg gtgatagttg ggcgtttgtc ggttcgaatg gaagcgggaa
     6961 atcggcgctg gcccgcgcgc aggcggggga acttccgctt ttgaaaggtg aacggcaaag
     7021 ccagttttcc cacatcactc gtctctcctt cgagcaattg caaaagctcg tcagcgacga
     7081 atggcagcgg aataacaccg atatgctcgg ccctggcgaa gatgacaccg ggcgtaccac
     7141 ggctgaaatc attcaggatg aagtaaagga tgcaccgcgt tgcatgcaac tggcgcagca
     7201 gttcggtatt accgccctcc tcgaccgacg ctttaaatac ctttccactg gcgagacgcg
     7261 aaaaaccctg ctgtgtcagg cgctgatgtc ggagcctgac ttgttgattc ttgatgagcc
     7321 gttcgatggc ctggatgttg cctcacgtca gcagctggct gagctactcg cctcgttaca
     7381 tcagtccggt attactctgg tactggtgct caatcgcttc gatgagatcc cggaatttgt
     7441 ccagtttgct ggcgtgctgg cggattgtac gttagcggaa acgggcgcta aagaggaact
     7501 gctccagcaa gcactcgtcg cgcaactggc acatagcgaa cagcttgaag gtgtgcaact
     7561 gccggagccg gatgaacctt cagcacgtca cgccttaccc gccaacgaac cgcgcattgt
     7621 gctgaacaac ggcgtggttt cttataacga tcgccccatt cttaataacc ttagctggca
     7681 ggtgaatcca ggcgagcact ggcaaattgt cgggccaaat ggcgcgggaa aatcgacgtt
     7741 attaagcctg attactggcg atcatccgca aggttacagc aacgatttga cgcttttcgg
     7801 acgacgtcgc ggcagcggcg aaaccatctg ggatatcaaa aagcatatcg gttacgtcag
     7861 cagtagtttg catctggatt accgggtcag cactaccgtg cgtaatgtga tcctttctgg
     7921 ctattttgat tcgattggca tttatcaggc cgtttcggat cgccagcaaa aactggtgca
     7981 gcagtggctg gatattctcg gcattgataa acgcacggct gacgctccgt tccatagtct
     8041 ttcctgggga cagcagcgtc tggcgctgat cgtccgcgcg ctggtgaaac atccgacgtt
     8101 gcttattctc gatgaaccac tacaggggct tgatccgctc aatcgccagc ttatccgccg
     8161 gtttgttgat gtgctgatta gcgaaggtga aacgcaattg ttgtttgttt cgcaccatgc
     8221 tgaagatgcg cctgcctgta ttacccatcg tcttgagttc gtgccggacg gtgactttta
     8281 tcgctatgcg ctgacaaaaa taaactgagt cggtaatgct gactgtaccg gaggcggcct
     8341 aagcgccctc tccggcctac agttcgacgc atccaggcat gaaaccgcga cttttttcag
     8401 ataaaaagct caatcattca taaaccctct gttttataat ccattaatgg cgcataaaaa
     8461 acggctaaat tcttgtgtaa acgattccac taatttattc catgtcacac ttttcgcatc
     8521 tttgttatgc tatggttatt tcataccata agcctaatgg agcgaattat gagagttctg
     8581 gttaccggtg gtagcggtta cattggaagt catacctgtg tgcaattact gcaaaacggt
     8641 catgatgtca tcattcttga taacctctgt aacagtaagc gcagcgtact gcctgttatc
     8701 gagcgtttag gcggcaaaca tccaacgttt gttgaaggcg atatccgtaa cgaagcgttg
     8761 atgaccgaga tcctgcacga tcacgctatc gacactgtga tccacttcgc cgggctgaaa
     8821 gccgttggcg aatcggtaca aaaaccgctt gaatattacg acaacaatgt caacggtacc
     8881 ctgcgcctga ttagcgccat gcgcgccgcg aacgtcaaaa actttatttt tagctcctcc
     8941 gccaccgtct atggcgatca gcccaaaatt ccatacgttg aaagcttccc gaccggcaca
     9001 ccgcaaagcc cttacggcaa aagcaaactg atggtggaac agatcctcac cgatctgcaa
     9061 aaagcccagc cggactggag cattgccctg ctgcgctact tcaacccggt tggcgcgcat
     9121 ccgtcgggcg atatgggcga agatccgcaa ggcattccga ataacctgat gccatacatc
     9181 gcccaggttg ctgtaggccg tcgcgactcg ctggcgattt ttggtaacga ttatccgacc
     9241 gaagacggta ctggcgtacg cgattacatc cacgtaatgg atctggcgga cggtcacgtc
     9301 gtggcgatgg aaaaactggc gaacaagcca ggcgtacaca tctataacct cggcgctggc
     9361 gtaggcagca gcgtgctgga cgtggttaat gccttcagca aagcctgcgg caaaccggtt
     9421 aattatcatt ttgcaccgcg tcgcgagggc gaccttccgg cctactgggc ggacgccagc
     9481 aaagccgacc gtgaactgaa ctggcgcgta acgcgcacac tcgatgaaat ggcgcaggac
     9541 acctggcact ggcagtcacg ccatccacag ggatatcccg attaaggaac gaccatgacg
     9601 caatttaatc ccgttgatca tccacatcgc cgctacaacc cgctcaccgg gcaatggatt
     9661 ctggtttcac cgcaccgcgc taagcgcccc tggcaggggg cgcaggaaac gccagccaaa
     9721 caggtgttac ctgctcatga tccagattgc ttcctctgcg caggtaatgt gcgggtgaca
     9781 ggcgataaaa accccgatta caccgggact tacgttttca ctaatgactt tgcggctttg
     9841 atgtctgaca caccagatgc gccggaaagt cacgatccgc tgatgcgttg ccagagcgcg
     9901 cgtggcacca gtcgggtgat ctgcttttca ccggatcaca gcaaaacgtt gccagaactc
     9961 agcgttgcgg cattgacgga aatcgtcaaa acctggcagg agcaaaccgc agaactgggg
    10021 aaaacgtacc catgggtgca ggtctttgaa aacaaaggcg cggcgatggg ctgctctaac
    10081 ccgcatccgc acggacagat ttgggcaaat agcttcctgc ctaacgaagc tgagcgcgaa
    10141 gaccgtctgc aaaaagaata tttcgccgaa cagaaatcgc caatgctggt ggattatgtt
    10201 cagcgcgagc tggcagacgg tagccgtacc gttgtcgaaa ccgaacactg gttagccgtt
    10261 gtaccttact gggctgcctg gccgttcgaa acgctactgc tgcccaaagc ccacgtttta
    10321 cggatcaccg atttgaccga cgcccagcgc agcgatttgg cactggcgtt gaaaaagctg
    10381 accagtcgtt atgacaacct cttccagtgc tccttcccct actctatggg ctggcacggc
    10441 gcgccattta atggcgaaga gaatcaacac tggcagctgc acgcgcactt ttatccgcct
    10501 ctgttgcgct ctgccaccgt acgtaaattt atggttggtt atgaaatgct ggcagaaacc
    10561 cagcgagacc tgaccgcaga acaggccgca gagcgtttgc gcgcagtcag cgatatccat
    10621 tttcgcgaat ccggagtgta agaaatgagt ctgaaagaaa aaacacaatc tctgtttgcc
    10681 aacgcatttg gctaccctgc cactcacacc attcaggcgc ctggccgcgt gaatttgatt
    10741 ggtgaacaca ccgactacaa cgacggtttc gttctgccct gcgcgattga ttatcaaacc
    10801 gtgatcagtt gtgcaccacg cgatgaccgt aaagttcgcg tgatggcagc cgattatgaa
    10861 aatcagctcg acgagttttc cctcgatgcg accattgtcg cgcatgaaaa ctatcaatgg
    10921 gctaactacg ttcgtggcgt ggtgaaacat ctgcaactgc gtaacaacag cttcggcggc
    10981 gtggacatgg tgatcagcgg caatgtgccg cagggtgccg ggttaagttc ttccgcttca
    11041 ctggaagtcg cggtcggaac cgtattgcag cagctttatc atttgccgct ggacggcgca
    11101 caaatcgcgc ttaacggtca ggaagcagaa aaccagtttg ttggctgtaa ctgcgggatc
    11161 atggatcagc taatttccgc actcggcaag aaagatcatg ccttgctgat tgactgccgc
    11221 tcactgggga ctaaagcagt ttccatgccg aaaggtgtgg ctgtcgtcat cattaacagt
    11281 aacttcaaac gtaccctggt tggcagcgaa tacaacaccc gtcgtgaaca gtgcgaaact
    11341 ggtgcgcgtt tcttccagca gccagcgctg cgcgatgtca ccattgaaga gttcaacgct
    11401 gttgcgcatg aactggaccc aatcgtggcg aaacgcgtgc gtcatatcct gactgaaaac
    11461 gcccgcaccg ttgaagctgc cagcgcactg gagcaaggcg acctgaaacg tatgggcgag
    11521 ttgatggcgg agtctcatgc ctctatgcgc gatgatttcg aaatcaccgt gccgcaaatt
    11581 gacactctgg tagaaatcgt caaagctgtg attggcgaca aaggtggcgt acgcatgacc
    11641 ggcggcggat ttggcggctg tatcgtcgcg ctgatcccgg aagagctggt gcctgccgta
    11701 cagcaagctg tcgctgaaca atatgaagca aaaacaggta ttaaagagac tttttacgtt
    11761 tgtaaaccat cacaaggagc aggacagtgc tgaacgaaac tcccgcactg gcacccgatg
    11821 gtcagccgta ccgactgtta actttgcgta acaacgcagg gatggtagtc acgctgatgg
    11881 actggggtgc gactttactt tccgcccgta ttccgttttc cgatggcagc gtccgcgagg
    11941 cgctgctcgg ctgtgccagc ccggaatgct atcaggatca ggccgcattt ctgggggcct
    12001 ctattggtcg ttatgccaac cgtatcgcca atagccgtta tacctttgac ggtgaaaccg
    12061 tgacgctttc accaagtcag ggcgttaacc agctgcacgg cggcccggaa gggttcgaca
    12121 aacgtcgctg gcagattgtg aaccagaacg atcgtcaggt gctgtttgcc ctgagttcag
    12181 atgatggcga tcagggcttc ccgggtaatc tcggagcgac ggtacaatat cgtctgaccg
    12241 acgataaccg tatctctatt acttatcgcg ccacagttga taaaccttgc ccggtgaata
    12301 tgactaatca cgtctatttc aatcttgacg gcgagcagtc tgacgtgcgc aatcacaagt
    12361 tgcagattct ggcggacgaa tatctgccgg ttgatgaagg cggcattccc cacgacggcc
    12421 tgaaatctgt cgccggaacg tcttttgatt tccgcagcgc caaaatcatc gccagtgagt
    12481 ttcttgccga cgacgatcag cgcaaagtga aaggttacga tcacgcgttc ttgttacagg
    12541 ccaaaggcga tggcaagaaa gtggcggcgc atgtctggtc agcagatgaa aaattgcagc
    12601 tgaaggtcta caccaccgct ccggctctgc aattctactc cggcaactac ctcggcggca
    12661 caccgtcgcg gggaaccgaa ccttacaccg actggcaagg gctggcgttg gaaagcgagt
    12721 ttctgccgga cagcccgaac caccctgaat ggccgcaacc ggactgtttc ctgcgtcctg
    12781 gcgaagagta ttccagcctg acggaatatc agtttattgc tcagtaatgt taagccctcg
    12841 tcatgagggc tttatcttca tattgttcaa atcaccagca aacaccgaca tatttgcaac
    12901 tcaatattca caacaacctt acactgcgac actattttcg ctatggttat gcgtaagcat
    12961 tgctgttgct tcgtcacggc aatataatga gaattattat cattaaaaga tgatttgagg
    13021 agtaagtata tggctgtaac taagctggtt ctggttcgtc atggcgaaag tcagtggaac
    13081 aaagaaaacc gtttcaccgg ttggtacgac gtggatctgt ctgagaaagg cgtaagcgaa
    13141 gcaaaagcag caggtaagct gctgaaagag gaaggttaca gctttgactt tgcttacacc
    13201 tctgtgctga aacgcgctat ccataccctg tggaatgtgc tggacgaact ggatcaggca
    13261 tggctgcccg ttgagaaatc ctggaaactg aacgaacgtc actacggtgc gttgcagggt
    13321 ctgaacaaag cagaaaccgc tgaaaagtat ggcgacgagc aggtgaaaca gtggcgtcgt
    13381 ggttttgcag tgactccgcc ggaactgact aaagatgatg agcgttatcc gggtcacgat
    13441 ccacgttacg cgaagctgag cgagaaagaa ctgccactga cggaaagcct ggcgctgacc
    13501 attgaccgcg tgatccctta ctggaatgaa accattctgc cgcgtatgaa gagcggtgag
    13561 cgcgtaatca tcgctgcaca cggtaactcg ttacgtgcgc tggtgaaata tcttgataac
    13621 atgagcgaag aagagattct tgagctgaat atcccgactg gtgtgccact ggtgtatgag
    13681 ttcgacgaga atttcaaacc gttgaaacgc tattatctgg gtaatgctga cgagatcgcg
    13741 gcgaaagcag cggccgttgc gaaccagggt aaagcgaagt aaacgtcatt cgcttaaaaa
    13801 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    13861 attgcctgat gctacgctgc gcgtcttatc agggctgcaa acgtacagtc atggcgattc
    13921 gactataaat tacccgcgac gcgcttttac tgcattcgcc agttgacgta acagagcatc
    13981 ggtatcatcc cagccaatgc aggcatcggt gatgctctta ccataggcca gcggttcccc
    14041 actctcgagg ctctgattgc cttccaccag atggctttcc accatcacgc caataatagc
    14101 cttttcgcca ccggcaatct gctggcaaac gtcagcacaa acatccatct gctttttgaa
    14161 ttgttttgac gagttagcat ggctgaaatc gatcatcacc tgtgctggca ggcctgcttt
    14221 gttcagccct tctttcactt cagcaacgtg cttcgcgctg tagttaggct ctttaccgcc
    14281 gcgcagaatg atatggcaat cgccgttacc gctggtattc acaatcgccg aatgccccca
    14341 tttcgttacg gacaggaagc agtgcggcgc accggcggca ttaatggcat cgatagccac
    14401 tttaatcgta ccgtcggtgc catttttgaa gccgaccgga caagaaagcc ctgatgccag
    14461 ttcgcggtgc acctgcgatt cggtggtacg tgcgccaatt gcgccccagc tcatcaggtc
    14521 agcgagatat tgtggggtga tcatatccag gaattcaccc gccgctggca gaccgctgtc
    14581 gttaatatca agcagcaatt tacgggcaat acgcagaccg tcgttgatct ggaagctgtt
    14641 atccatatgc ggatcgttaa tcagcccttt ccagcccacc gtggtacgcg gcttttcaaa
    14701 atagacgcgc atcacgattt ccagctcatc ttgcagctct tcacgcagcg tcagcaagcg
    14761 agtggcatac tctttcgccg cgacaggatc atgaattgag cacggaccaa tcaccaccaa
    14821 caggcgatca tcattacctt tcaggatctt atggatcgct tttcgggcat gggcgaccgt
    14881 attcgcggca ttttcagtag cggggaattt ttccagcaat gcgactggag gaagtaactc
    14941 tttgatttct ttgatgcgta aatcgtcgtt ctgataattc atgtctgttc cagtgttgcc
    15001 atacttatct tagtgaatgc aatacttcca atctatatct tccgtcagaa tgtgtaaacg
    15061 gggttttaca ctatgaacgg attaatcctg gaattagcaa gaaaatcgcc agattgtcgc
    15121 gaaaaacgag atctctccta caatttctaa ctgtaactcc tttgtttgtt aattatttca
    15181 agattctctg ctgggtatct ttgcctggct gaaatcttaa accattgcct tatattcacc
    15241 tgcaaatgca ctgttggaag aggttatccg acataacgac cataacagga gcatcctatg
    15301 aaaatgacaa aactggccac actatttttg actgccactc taagccttgc cagcggtgcc
    15361 gcactggctg ccgatagcgg agcgcaaagc aataacggcc aggcaaacgc cgcagctgat
    15421 gcgggccagg tagcccctga tgcccgtgaa aatgtcgcgc caaataacgt cgacaataac
    15481 ggggtaaata ccggttctgg cggcacaatg ctgcatccgg atggttcttc aatgaacaat
    15541 gacggcatga ccaaagatga agagcacaag aacaccatgt gcaaagatgg tcgctgcccg
    15601 gatatcaata aaaaagtcca aaccggtgat ggcatcaaca atgatgtcga taccaaaacc
    15661 gacggtacca cgcagtaacc ctgcgaaata gccaataatg ggagagcgga gtctctccct
    15721 tttaattttt cccttctaaa agcagtatga tgtcataaag ttaagtgaga ataattaagg
    15781 gatgactcgt catggcgcac tcacactcac acacttcctc acacctgcca gaagataata
    15841 atgctcgtcg cttgttgtat gctttcggcg tgactgccgg gtttatgctc gtagaagtca
    15901 ttggtggttt tctttccggt tctctggcat tgctggccga tgcgggtcat atgttgaccg
    15961 atactgccgc cctgcttttc gccctactcg cagtgcaatt ttcccgtcgt cctcccacca
    16021 ttcgccacac tttcggttgg ctaagattga ccacgctcgc ggcgtttgtg aatgccatcg
    16081 ccctggtggt gattactatt ttgattgtct gggaggcgat agaacgcttc cgcactccgc
    16141 gtccggtcga gggcggcatg atgatggcaa ttgccgtggc tgggctgctg gcaaatatac
    16201 tttctttctg gttacttcat cacggcagcg aagagaaaaa cctcaacgtg cgagcagcgg
    16261 cactgcatgt gctcggggac ctgctgggtt cggttggggc tattattgcc gcgttgatta
    16321 ttatctggac cggctggaca cctgctgacc ccattctctc aatactggtg tcgcttctgg
    16381 ttctgcgtag cgcgtggcga ctgttgaaag atagcgtgaa tgaattactt gaaggtgcac
    16441 cggtatcgct ggatatcgcc gaactgaagc gtcgtatgtg ccgggaaatc ccggaagtcc
    16501 gcaatgtgca ccatgtgcat gtatggatgg taggcgagaa gccggtgatg acgctgcatg
    16561 tgcaggtgat cccgccacac gatcacgatg ccttgttaga tcagatccaa cactacctga
    16621 tggatcacta tcagattgag cacgccacta ttcagatgga atatcaacct tgtcatgggc
    16681 cagactgcca cctgaatgaa ggggtgtctg gccattcaca tcatcaccat taatgggaca
    16741 gcgcgcgtga acctctttcg cgtgcgctgt tgatccacat ccggctgccg ttgagggcaa
    16801 taaaggtcag gatgatgtac tccagagaca tggcgtaaac gccctgaagt gcaaaaataa
    16861 cgacgctaat cacgttaata atcacccaca acagccagtt ttccacatac ttacgcgtca
    16921 tcagaatcat tgccacgata gataacacca tcatgcatga atcccagaac gggaaagcgt
    16981 ccggctgtag ttcaggcatc gcaacctgta atcctaatgc ttgcatgatc atgactgcca
    17041 cgcgggtcaa aaatgcaaac accggattga taaataccgt catcagacca atcgaaacaa
    17101 cgcaaaccgc caaccagctt agcgccttcg gcaatggcaa ccagcgaatt ttcaactccg
    17161 cctcgttctg actggtttgt cgcgaccacg cataccagcc gtaaatattc gcggcaaaga
    17221 aaaacatctg taatagcagg ctggcataca gctgaatctg aaagaaaata atgccaaaca
    17281 aggtgacgtt gatcaggcca aagaagtagt tgctgatctt ctccagactg gcaaggccaa
    17341 tacacagcaa cccggcgatc gtgcctaccg cttcgatcca tgagagatca taaccgcctg
    17401 cacctattgg tatatgtacc aggatattct gcacactaaa aaaatccatc tttcccccaa
    17461 agcgtaacgt tacgccttat tattcgttat ccacgtagtg tagccgcaaa atccagcata
    17521 cgattgagcg gcaccagcgc cctctctcgc agcctttcat caacatgaac ctcgtgattg
    17581 cttccttcct gttctaatgc ctctgcgatg gcctgaaggc cattcatggc catccacgga
    17641 caatgcgcgc agctgcggca ggttgcaccc tcacctgcgg ttggtgcttc cagtaactct
    17701 ttaactggca ccgcctgctg cattttgtag aaaatacccc gatcggttgc cacaataagc
    17761 ctctgatgtg gcaatgtttt cgcagcagcg atcagttgac tggtggaacc gaccgcatcc
    17821 gccatctcta caatagcttg cggtgattct ggatgcacca gtatggcagc atccgggtat
    17881 tcttcttgca agcgggttaa cgcctgagtc ttaaactcat catgcacaat gcaggcaccc
    17941 tgccagcata atatgtctgc gcccgtctgt ttttgcacgt aacgccccag atgtttgtcg
    18001 ggtgcccaga tgattttttc acccaaacta tccagatgat caataagttc gacggcaatg
    18061 cttgaagtta ccacccaatc tgcgcgcgct tttaccgcag cagaagtgtt ggcgtagacg
    18121 acgacagtac gatcgggatg ggcatcgcaa aatgcgttaa attcttcaac agagcagccg
    18181 agatccagtg aacattcagc ctgaagtgtc ggcatcagaa ttgttttttc cggactgaga
    18241 attttggcgg tttctcccat aaatctcacc ccagcgacta acaacgtaga agcgggatgc
    18301 tttgcaccga agcgcgccat ttccagagaa tcagaaatac agccaccggt ttcttctgcc
    18361 agttgttgaa tttcgggatc ggtatagtag tgggcaacca tcaccgcatt acgttctttt
    18421 agcagacgtt ttatcttctc gcggtaatac gctttttcat caatgcttaa cggcgtcggc
    18481 ttcgggggaa aaggataaat cgccgtgtct ggatcaaaca tcacgctcat cttaccatct
    18541 cgttttacag gcttaacgtt aaaaccgaca tgagcgtaat attcgctgtt atcggtgaaa
    18601 tttgttttat atgctaaaca agatagtcga atggtgggga aaagtcactc gaattacgcc
    18661 taattccacc gacgtagagt tgcttgcaaa gtaagctctg aagatgtcat caggttacaa
    18721 agtgaaattg aggatgcctg aatagcaaaa aagcgccttt agggcgcttt tttacattgg
    18781 tgggtcgtgc aggatgactc gcttcgctcg ccctacgggc cgtcgccgcg agcggctccg
    18841 ttatttcgct gcgctcaact cgaacctgct gcaggttcga atctttgagt at
//
LOCUS       DAZGHF010000090        18639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGHF010000090 DAZGHF010000000
VERSION     DAZGHF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.090"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..945
                     /locus_tag="ACHK3A_003299"
     CDS             1..945
                     /locus_tag="ACHK3A_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO8741345.1"
                     /translation="MPLLLSLTFIIFYQPWVRALPPTPRHASPEQLEKTVRYLTQTVH
                     PRSADNIDNLNRSAEYIKEVFISSGARVTAQDVPITGGPYKNIVADYGPADGPLIIIG
                     AHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQLQTPGIGVQLVAYASEEPPF
                     FRSDEMGSAVHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLYPDRGDFIAV
                     VGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDT
                     AFYRNKQYHLPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            1048..1551
                     /locus_tag="ACHK3A_003300"
     CDS             1048..1551
                     /locus_tag="ACHK3A_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO8741346.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1618..3075)
                     /gene="nuoN"
                     /locus_tag="ACHK3A_003301"
     CDS             complement(1618..3075)
                     /gene="nuoN"
                     /locus_tag="ACHK3A_003301"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO8741347.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3082..4611)
                     /gene="nuoM"
                     /locus_tag="ACHK3A_003302"
     CDS             complement(3082..4611)
                     /gene="nuoM"
                     /locus_tag="ACHK3A_003302"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO8741348.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4775..6616)
                     /gene="nuoL"
                     /locus_tag="ACHK3A_003303"
     CDS             complement(4775..6616)
                     /gene="nuoL"
                     /locus_tag="ACHK3A_003303"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO8741349.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6613..6915)
                     /gene="nuoK"
                     /locus_tag="ACHK3A_003304"
     CDS             complement(6613..6915)
                     /gene="nuoK"
                     /locus_tag="ACHK3A_003304"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO8741350.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6912..7466)
                     /gene="nuoJ"
                     /locus_tag="ACHK3A_003305"
     CDS             complement(6912..7466)
                     /gene="nuoJ"
                     /locus_tag="ACHK3A_003305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO8741351.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7478..8020)
                     /gene="nuoI"
                     /locus_tag="ACHK3A_003306"
     CDS             complement(7478..8020)
                     /gene="nuoI"
                     /locus_tag="ACHK3A_003306"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO8741352.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8035..9012)
                     /gene="nuoH"
                     /locus_tag="ACHK3A_003307"
     CDS             complement(8035..9012)
                     /gene="nuoH"
                     /locus_tag="ACHK3A_003307"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO8741353.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9009..11735)
                     /gene="nuoG"
                     /locus_tag="ACHK3A_003308"
     CDS             complement(9009..11735)
                     /gene="nuoG"
                     /locus_tag="ACHK3A_003308"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO8741354.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11788..13125)
                     /gene="nuoF"
                     /locus_tag="ACHK3A_003309"
     CDS             complement(11788..13125)
                     /gene="nuoF"
                     /locus_tag="ACHK3A_003309"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO8741355.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEVGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13122..13622)
                     /gene="nuoE"
                     /locus_tag="ACHK3A_003310"
     CDS             complement(13122..13622)
                     /gene="nuoE"
                     /locus_tag="ACHK3A_003310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO8741356.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13625..15427)
                     /gene="nuoC"
                     /locus_tag="ACHK3A_003311"
     CDS             complement(13625..15427)
                     /gene="nuoC"
                     /locus_tag="ACHK3A_003311"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO8741357.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15521..16183)
                     /gene="nuoB"
                     /locus_tag="ACHK3A_003312"
     CDS             complement(15521..16183)
                     /gene="nuoB"
                     /locus_tag="ACHK3A_003312"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO8741358.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16199..16642)
                     /gene="nuoA"
                     /locus_tag="ACHK3A_003313"
     CDS             complement(16199..16642)
                     /gene="nuoA"
                     /locus_tag="ACHK3A_003313"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO8741359.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17272..18210)
                     /gene="lrhA"
                     /locus_tag="ACHK3A_003314"
     CDS             complement(17272..18210)
                     /gene="lrhA"
                     /locus_tag="ACHK3A_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO8741360.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4637 a         5170 c         4826 g         4006 t
ORIGIN      
        1 ataccattac tactatcgct tacttttatt attttttacc aaccctgggt cagggcatta
       61 ccaccaacgc cccgacacgc tagtcctgag caattagaaa aaacagttcg ttatcttaca
      121 caaactgtgc atccacgtag cgccgacaat attgataatc tgaataggtc cgcagagtat
      181 ataaaagagg tctttatcag tagcggtgcc agagttaccg cgcaggatgt ccccattacg
      241 ggtggcccct acaaaaacat tgttgctgat tatggtcctg ccgatggacc gctgattatt
      301 attggtgcgc actatgactc tgtcagtagt tatgaaaacg atcaattgac ctatacgccg
      361 ggcgcggatg ataacgccag cggcgtggca ggattactcg aactggcgcg tttgttacag
      421 ctgcagactc cagggattgg cgtccaactg gtcgcctatg cgtcggaaga accgcccttc
      481 tttcgtagcg atgaaatggg gagcgcggta catgcagctt cgcttgagcg tccagtgaaa
      541 ttaatgatag cgctggagat gattggttat tacgactcaa cgcctggtag ccaggattac
      601 ccatccccgg caatgtcctg gctttatccc gatcggggag actttattgc cgtggtcggc
      661 agaatgcagg atatcaacgc cgttcgtcag gtaaaagcgg cattgttgtc atcccgggat
      721 ttatctgttt attctatgaa tgccccaggt tttattcccg gtattgattt ctctgatcac
      781 cttaattatt ggcaacacga tattcccgcc gtaatgatta ctgacaccgc tttttatcgt
      841 aataaacaat accacttgcc cggtgatatc gcagacagat taaattatca gaaaatggct
      901 caggtagtag atggagttac aaccttgtta tacaacagta aataataaaa atttctcgag
      961 gcggatccag aaaaagcaac atgcgcattt tattttcttt cgtctgtttt aagatgcgaa
     1021 acatgaaatt caacggagga aaaataaatg ggaatgattg gctattttgc agaaattgac
     1081 tcagaaaaga ttaaccaact gcttgagtct actaaaaaac ctttgatgga taatatacat
     1141 gacacgcttt caggattacg ccgactggat attgataaac gctgggattt tttacacttt
     1201 ggcctgacgg gtacatctgc atttgacccc gcgaagaatg acccgctaag tcgcgcagtg
     1261 cttggtgaac acagcctaga agatggcata gatggcttcc tgggattgac ctggaatcag
     1321 gagttagccg cgacaatcga ccgactggaa gctctggatc gaagtgagct gcgtaaacag
     1381 ttttccatta agcgactcaa tgaaatggag atttatccgg gagtaacctt ttcagaagag
     1441 ttagaggggc aactctttgc ttcgatcatg cttgatatgg aaaagctgat tagcgcgtat
     1501 cgacgtatgc tgcgacaagg gaatcatgcg ctgacggtta ttgttgggtg atggtgtcag
     1561 ggcaccaaaa aaaaccgccg aacatgtcgg cggctttctg acttacaaac tgactgctta
     1621 catcagcggc attgctaacc gcacaatgct aatcagcggt tgtggccata cacccagcac
     1681 cagtaccaac agtgcagaga tcagcaccac gataccgcct gcgctgtact gccagtttga
     1741 tggtgcatcg cgacccggtt gttccggagc gtgaaggtat aagctcaccg ccacgcgcag
     1801 gtagtagtag aggccgattg ccgaaccgac aactacggca cccaccagcc accacaagtg
     1861 tgcctggaca ccgaccgcca gcacgtagaa cttaccgata aagcccagcg tcattgggat
     1921 accagccagt gaaagcatca tcaccgtcat cactgccgcg aggatcggac gatgccagaa
     1981 cagaccgcgg taagagaaca gggaatcagc atccgggcca cgatacgggc tggacatcag
     2041 gctgaccacg ccgaacgcgc cgaggctgct gaacagataa cctgccaggt aaacccctac
     2101 cgcttccatc gacatctcgc cagtttgcag cgcaatcagc gccaccagca gatagccgag
     2161 gtgagagata gatgagtaac cgagcagacg tttgatattg gtctggctca gcgccatcag
     2221 gttaccgaag atgatggagg caaaggcgat aatcgccagc accacgcgaa tcgcttcgct
     2281 gtcacccacc ggtgcgtaga ggaacagacg catcaccaca ccgaagatag cgattttgct
     2341 cgccgtcgcc aggaaagtgg aaaccggcgc aggcgcgccc tggtatacgt ctggcgtcca
     2401 caggtggaac ggcaccagag agagtttgaa gccgaggccg acaatcatca ggccgaaacc
     2461 tgccagcaac agcggctcgt tgagcatacc gtcgccgagg tttttaccca gcgcgacaaa
     2521 cgacaggtcg ccagactgcg catacaccag cgccataccg aacagcagga aagaagacgc
     2581 tgcggcagag agaatggtgt atttgatact ggcttccagc gaacgtttct ggcggaaagc
     2641 gtaaccgacc aggccaaaca gcggcaaaga gatcagttcg ataccgagga acagagacgc
     2701 cagatggttg gcattcgcca gcaggatccc gcccagggcg gcaattaaca ccaacaggta
     2761 gaactcgtcc ttgttgtcgt tatagccttc aagccacggg taggcgaaag tacaggtggc
     2821 gaggctcgcc aacaatacca gcccggtgta aagcatggcg aaaccatcaa cgcgcatcag
     2881 tggcgtaacg tccatagcgc ccgcctggcc gacaaaccag agcgaaacca gcgccgcgtt
     2941 aagcccaata accgagagcg tagcgttgag gaaatgattg cgtcgccacg caatggagag
     3001 catcaccacc accaccgtca agccgacgat cagcaacggt agcagtgcga tcaggttttg
     3061 tggagttatt gtcatggcga tttacggcct tgtagtagta acggaattaa caaaccactg
     3121 ctggatattg ccaatcgcgg agtgcgaggt atccagaatc ggctgcggat agaagcccag
     3181 cagtaccagc agcaccacca gcaacaggat cataaacagc tcacgcagcg acatccctgg
     3241 cagttcctgg ctggcaatct ggcttttcgc tttaccgaag taagcgcgat gtaacatcgc
     3301 cagcgaataa acagatgcaa agaccagccc aaaggtagag ataacggtaa tcaccgggac
     3361 aacctggaag ctgccgaaca gaatcataaa ttcgccgacg aagttaccgg tgccaggcat
     3421 cccaagcgtt gccaccgcaa agaacagcga cagtgctggc agccatttca tcttgctcca
     3481 cagaccgccc atcatgcgca tgtcgcgggt atggatacgt tcataaagct gaccgcagag
     3541 aataaacaga cccgccgccg acaagccgtg cgcaatcatc tggattaccg cgccctggta
     3601 ggccaactgg ctgccagtgt agatagcaat cagcacgaag cccatgtggg aaaccgaggt
     3661 gtaggcgatc agacgtttga tatcggtctg ggcgaaggcc atccacgcac cgtagaagat
     3721 gccgataaca cccagccaca tagcgattgg cgcgaactct gccgacgcgt tcgggaacaa
     3781 cggtagggag aaacgcagca gaccgtaagc ggcggttttc agcaagatcc ccgcgaggtc
     3841 aacggaaccg gcggttggag cctgtgagtg cgcatccggc agccagccat gcagcggaac
     3901 caccggcatt ttcaccgcaa atgcgatgaa gaagcccagc atcaacagat attccacacc
     3961 actggacatt ggcgtattca acagctcttc atagttgaag gtccagacgc cggtcgcatt
     4021 gtagtgaaca aaaaccagcg ccaggatggc gatcaacatc accagaccac tcgcctgggt
     4081 gtaaatgaag aacttggttg ccgccgtgat acgcgtttta ccgtcagagg ctttatgccc
     4141 ccacagtgcg atcaggaagt acatcggcac cagcatcatt tcccagaaga agaagaacag
     4201 gaacatgtcg atggcaagga acacgccgat aacgccgccc aggatccaca tcaggttgag
     4261 gtggaagaag ccctgatatt tttcgatctc tttccacgaa cagagtaccg ccagcacacc
     4321 gagcagaccg gtcagcacga ccatcagcag cgacagcccg tcaatggcga gatggataga
     4381 gataccaaaa cgcgggatcc acggcatgtc gaattcagac tgccactgcg gaattccggc
     4441 ggattgcgtc agtgaataac cgccctgcaa ccacagttgc agcgacagcg ccagcgtcaa
     4501 tcccatggtg atcagcgcga tccagcgcgg caccttgacg ccaaagcgtt cggtctgcca
     4561 gcacaggaag ccgccgataa agggaattaa tattagccag ggtagtaaca tggcgatctt
     4621 tattccttgt aaaagtcccg tcaggaccgg attttcaacg aattctcacg acagaaaatt
     4681 ctcttcggac tgggggtagt gtcggatgca ccgcgaatgc ggcttatccg acctacgttc
     4741 ttatatgccc aggggcaatc ccacaatcct taactcaacg cagtaccatc aacagtgcca
     4801 gcaccacgac cgcaccgatg ctcatggatg ccacatacca gcgcagataa ccgttctcgc
     4861 ttaacagcag acctttacct gcaaagcggg aaaggacagc cgggatgttc atcattgagt
     4921 tcagcggatc gcgtttcagc aaccaggcaa tacccaggaa cggcttgacg aacactttgt
     4981 cgtacagcca gtcaaagccc caggcgttgt accaccaggt acccagcaga cggcccggcg
     5041 cactgttggc gatggaggtc accagagtac gtttacccag ccacagccag gcagccagca
     5101 gaatgccgac caccgcgacc acgccagagg taatttccag ggtcaacatg ctgccgtgcg
     5161 ccagttccgt cgtttgcgga agcacgccct gcagcggcgg tacaatcagt gcgccaacga
     5221 aggtggaaag gatcagcagc acaatcagcg gcaggctgtg agttacccct ttcacggcgt
     5281 gagcgtgaat ttgttctttt ccgtggaaga cgatgaaaat catacggaag gtgtagagcg
     5341 aggtcataaa cgcaccgacc agacctgcca ccatcagatt gatatgacca ttcgccatcg
     5401 cacccgcgag gatctcatcc ttactgaaga agcccgcagt gaccagcggc agtgccgaca
     5461 gtgctgcgcc gcccaccagg aagcagagat aaaccagcgg aatagattta cgcagaccgc
     5521 ccatcttgaa gatgttctgt tcgtgatggc aggccagaat gacggaaccg gatgccagga
     5581 acagcagcgc tttaaagaac gcgtgggtca tcaagtggaa aatcgccgca tcccatgcct
     5641 gcacgccaag cgcgaggaac atgtagccaa tctggctcat ggtagagtaa gcgagaacac
     5701 gtttgatatc ggtctgtacc agcgcggcaa aaccggccag cagcagcgta accgccccga
     5761 caatgcccac cagatgcaga acttccggcg tcatcaggaa caggccgtgg gtacgagcga
     5821 tcaggtagac acccgcggtt accatcgttg cggcgtggat cagcgcggag acaggcgtcg
     5881 ggcccgccat cgcgtcggca agccatgtct gcaacggcaa ctgcgcagat ttaccgaccg
     5941 caccgcccag cagcatcagc gtcgcccaca tcagcatgtt attgccgtca gcaaagtgcg
     6001 ctggtgccag ttccaccatt tcgcggaagt tcagggtgcc cagttcgttg taaagaatga
     6061 acagcgcgaa agcgaggaac acgtcaccca cacgggtcac gacgaacgct ttcattgccg
     6121 ctgcgccatt cttcggatcg gtgtaataga aaccgatcag cagataggag cacaggccca
     6181 cgccttccca gccgaggtac atcaacagta ggttatcggc gagaaccaga actaccatgc
     6241 tggcgatgaa caggttggtg taagcgaaga agcgagagta gccctcttct ccgcgcatat
     6301 accaggaggc atacatgtga ataaggaaac ccacaccagt gaccactgag agcatggtca
     6361 gcgacaggcc gtccagcacc agattgaaac caatgttaaa gtcgcctacc gacatccacg
     6421 tccacagcgg ctggctgtat gcctgctcac cgttagcgaa gaagtcaacg ccgataaagg
     6481 cggttaccag cgccgccagg cccacagagc caacgccgac gatcgccgag acgttttcag
     6541 accagcgccc acgggagaac gccagcagga cgaagccaat caatggcaaa ataatggtta
     6601 aggcaagcat gttcatccgc gcatctcact tactgaatcg atgttcaggt tctggcgacg
     6661 acggtgaagt tgcagcagca gcgcaaggcc gatactcgct tccgccgccg cgaggctgat
     6721 ggcgagaatg tacatcacct gaccgtcggt ctggccccag tagcttccgg cgaccacaaa
     6781 ggccagcgca gaggcgttaa tcatgatttc cagaccaatc agcataaaca gcagattgcg
     6841 acggataacc agaccggtta agccaagaac gaataagatt gccgcgagga tcagtccatg
     6901 ttgtaagggg atcatgcgtg ctcctccgtt tttcttttcg cgctgtcgtc tttacgattg
     6961 ctcagcactt cacccgcacg ctcttcacga ccgacgtgga aggccacaac cagacccgcg
     7021 agcagcagca tagaagccag ttccaccgcc agtacgtaag gcccgaacag cgtaataccc
     7081 actgctttag cactgattgg cgtaccgtcg ataccctgat cattaacacc aaggatggcg
     7141 taaacaatca ccaccagcat gatggccgac aaaatcgccg gaccaatcca cacctgcggt
     7201 ttcagccact ggcgttcctg ttcgatttct gaaccgccca ggttgagcat catcaccacg
     7261 aacacgaaca gcaccataat ggcacccgcg tagacgataa tttccagcgc accggcgaag
     7321 taagcgccca gtgagaagaa caccccagaa atcgccagca gcgaaataat caggtacagc
     7381 agcgcgtgta ccggattggt atgggtgatc actcgcaagg tcgcaagtat ggctatcagg
     7441 ccacagatat aaaaagcgaa ctccattgcc cctctcctta cggtaacagg ctcttgacgt
     7501 cgataggctt ggcttcgttc tctgcttcgc ccttatcttt gccgtcgatt gccatacctg
     7561 ccatccggta gaagttatat tccgggtatt tgcccggacc ggagatcagc agatcctctt
     7621 tctcgtaaac cagatcctgg cgcttgtatt cccccatttc gaaatccggg gttaactgaa
     7681 tcgccgtggt cggacaggct tcttcgcaca gaccacagaa aatgcagcgt gagaagttga
     7741 tgcggaaaaa ttccggatac cagcgaccgt ctttggtttc tgctttttgc agcgagatac
     7801 agccgaccgg gcaggctacc gcgcagaggt tacaggctac gcaacgctct tcgccgtccg
     7861 gatcgcgggt cagaacgata cgaccacgat aacggggcgg cagatagacc ggctcttccg
     7921 ggtacattcg cgtttcgcgt ttggcgaacg cgtgcaggcc gatcatccag atactacgaa
     7981 cctgggtgcc gaaacctact aacaattctt ttaaggtcat ggtcttattg ccccttattg
     8041 cgcctgccag agaatgacag ccgccgttac cagcaagttg atcagcgtca gcggcaggca
     8101 gattttccag ccgaaggaca ttacctggtc ataacgcgga cgcggtaacg acgcacgaat
     8161 caaaatgaac atcatcataa agaacgcggt tttcagcgcg aaccagatga atggcggtaa
     8221 caacgggcct tgccagccac cgaagaacag cgtcaccatc agtgcagaga tggtcacaat
     8281 cccgatgtat tcgcccacga agaacagacc gaacttcatg ccggaatatt caatgtggta
     8341 accatccgcc agttcctgct cggcttccgg ctggtcaaac gggtgacggt gacacaccgc
     8401 cacgcccgcg atggcaaagg taataaaacc aaagaattgc gggatgacgt tccacacatg
     8461 cgcctggctg ttgacgatgt cggtcatgtt gaatgaaccg gcctgcgcca ccacgcccat
     8521 cagggaaagc ccgaggaaca cttcgtagct cagggtctgc gcagaagcac gcatcgcacc
     8581 cagcagcgag tatttgttgt tacttgacca gcccgcaaac agcaccgcgt aaaccgccag
     8641 accggccatc atcaggaaga acaaaatccc gatgttcagg tcggcaacca cccaacccgg
     8701 actgactggc acaatcgcaa aggccagcag cagcgaggta aaggcaatca tcggtgccag
     8761 ggtaaagatg acgcgatccg agaatttcgg gatccagtct tctttaaaga acatcttgat
     8821 catgtccgcg accagctgga gcgaaccgcc ccagccaaca cggttaggtc cgtaacggtt
     8881 ctggaacaga cccagcaggc gacgttcgcc aaagctcatg aatgccccac aggtgacaac
     8941 caccagcagg atcaccaccg ctttgaggat ggtcagcagg atctcaatca gttccggtga
     9001 tatccaactc attgttgtgc ctccttgaga tcctcaagat gcgcgccagc cagcaccgga
     9061 gcaatgccgg acatacccat cggcaagccc acctgccctg ccgtcagtcc ttcggcgatt
     9121 tcaaccggca gcgtgaccgt gttgccatcg taactaaagg agacgcgtgt acccgcgttc
     9181 acacccaact tcgcggcatc cgctgggttg agtttgatgt acggctgcgg catacggctc
     9241 tggaagaccg gagcacgctg tgacaattca tcgctaccaa acaggtggta gtacggcgcg
     9301 atacgccatt tcccgtcctg cggctggaag cgtgccggta cgctggtgaa gtaatccaga
     9361 ccattttcgc tggtttcaaa cagacgcacg cccggatcgc caaagcgcag tttgccgccc
     9421 acttcgtcct ggaatttgtt ccacgcctgc ggggagttcc agcctggcgc ccaggcaaac
     9481 ggcacttgcg aacggtgcgc ggtcggctgg ttgttacctt ccatcgagaa ggtgaacatg
     9541 gtgtcaatat cctgcggctg acgcggctca tgaacgctga tattggcgcg catggcggta
     9601 cgaccgctgt aacggtgcgg ttcacgggcc agtttctgcc cacgaatacg gaatgtcgca
     9661 tccggcgcag catctttgat acccgccagt tccgggattt tcgccacaac agcgtcaatc
     9721 acatggtcga gctgcgtcca gtccacttca cggctcagca aggtgctgtg cagcgagtgt
     9781 aaccaacgcc agctttccag catgacagtt ttgctgtcgt aataagccgg atcgtaaacc
     9841 tggaagaaac gttgggcacg gccttcgttg ttaatcaccg taccgtcgct ttcagcaaag
     9901 ctggcagcag aaagtaccag atgggcgttt tccataatcg ctgtgcgttg atggtcaacc
     9961 accatcacca gcggcgcttt agccagcgca gcattcacgc gggtagcgga agcgtgacga
    10021 tgcagatcgt tttccagcac caccaccgcg tcggcgcgtc cggtttccag ttcggttaac
    10081 gcttcttcaa gcgatccacc gcccataatg cccagcccca tgctgttgac ggaacgggca
    10141 atcatggtga taccgacgtc agcgccgcga cctttcaggg ctttcgccac gttagccgca
    10201 gcctgaatca cttcagcgct accggcgttc gtcccggaga taatcaacgg tttcttcgca
    10261 cctgccagtg cctgcacgat gacgtcgatt ttgctttgca gctcaggttc aataccgtca
    10321 accgctggcg cggagttatc cagcgcatgg gcgatggcaa aacctaaacg cgcctgatct
    10381 tcaaccggtg cgcggtaagt ccacgccgcg atatcatcca gacgggtgtc atcaacgttg
    10441 gtaacaaaca gcggatgctt cgcacgttgg ccgatgttga ggattgccgc aatctgccag
    10501 tcagccactt tctgtgctgc cgccatttcg cgcgctttac ctttcaccgc ctgacgaact
    10561 gccagcgcga cgcgcgcgcc ggtctgggta acgtcttcgc ccagcaccag taccgcatcg
    10621 taagattcga tttcgcgcag tgccggcgta taaatgccgc cttcacgcag cactttcagc
    10681 gccagttgca gacgttcctg ctcaccgtga gcgataccgg tgtagaagtt ttcttcgccc
    10741 accagctcac gcagcgcaaa gttgctttcc acgctggcac gcggagaacc aatgccgatc
    10801 actttcttcg actgacgcag aatatctgcc gcgccctgca ttgcctgttc ggcgttgagg
    10861 gtaatgaaat catcgccacg acgctgtact ggctgacgcg gacgatcttt caggttgacg
    10921 taaccgtaac cgaaacgacc acggtcgcag aggaagtagt ggtttaccgt accgttgtaa
    10981 cggttttcga tacgacgcag ttcgccgtaa cgctcgccgg ggctgatgtt acagccgatg
    11041 gaacattgct ggcagatgct cggcgcaaac tgcatatccc atttacggtt gtaacgctcg
    11101 gagtgcgttt tgtcggtgaa tacgccggtc gggcaaatct cgaccagatt accggaaaat
    11161 tcgctttcca gcgtgccgtc ttccgggcga ccgaagtaga cgttgtcgtg cgcaccgtaa
    11221 acgcccaggt ccgtgccgtc agcgtaatct ttgtagtaac gcacgcagcg gtagcaggcg
    11281 atgcagcggt tcatttcgtg agagatgaat ggccccaaat cctgattacg gtgggtacgt
    11341 ttggtgaaac ggtagcgacg gaagctgtgt ccagtcatca cagtcatatc ctgaagatgg
    11401 cagttaccgc cctcttcaca taccggacag tcgtgcgggt ggttggtcat taaccactcg
    11461 accacacttt cacggaactg tttcgcttct tcatcgtcaa tggaaataaa ggtgccatcg
    11521 gaagccggtg tcatacagga catcaccagg cgaccacgcg tgtcttccgc gttttggtat
    11581 tgcttcaccg cacactggcg gcaagcaccg acgcttccca gcgccggatg ccagcaaaag
    11641 taaggaatat caaggcccag agacagacaa gcttccagca agttgtccgc tccgttgacc
    11701 tcgtattctt tgccgtctac atgaattgta gccattagca tgcttccagt tttctcagtc
    11761 agagactgag cgttaatcga aattcggtta ccagcgctct ttcagcaggt tcggctgaat
    11821 cccattaatc aaatgggtat tgctgaacgg ctgtttgatt cccgcctcaa attcttcgcg
    11881 gaaatatttg atggcgctct gtaacggctc cactgcacca ggtgcgtggg cacagaaagt
    11941 tttacccggg cctaagaatc gacacagttg ctcaagtgtt tcgatatcgc ccggctgacc
    12001 ttcgccacgc tccagcgcac gcagaatttt cacgctccac ggcagaccgt cgcggcacgg
    12061 cgtacaccag ccacaggact cacgggcgaa aaactcttcc aggttacgca ccagcgacac
    12121 catgttgatc tcatggtcaa ccgccatcgc cagcgccgta cccagacggc ttcccgcttt
    12181 accgatactt tcgaattcca tcggcagatc aaggtgcgct tcggtcagga agtcggtgcc
    12241 agcgccgcct ggctgccagg ctttgaattt cagaccgtca cgcataccac cggcgtaatc
    12301 ttcgaggatc tcgcgtgcgg tggtgccgaa tggcagttcc cacaggcccg gatttttcac
    12361 ccgaccggag aagcccatca gcttggtgcc agcatcttta cttttcgaga tgttctgata
    12421 ccactccacg ccgttagcga ggatcgccgg aacgttacac agggtttcga cgttattgac
    12481 acaggtcggt ttgccccatg caccggaggt tgccgggaat ggaggcttcg agcgagggtt
    12541 agcgcgacgc ccttccaggg agttgattaa cgctgtttct tcaccgcaga tgtagcgccc
    12601 agccccggta tggacgaaca attcgaagtc gaaacctgtt cccataatgt ttttgccaag
    12661 cagacccact tcggtggctt cggcaatggc acggcgcaga ttcactgccg cttcgatata
    12721 ctcgccacgc aggaagatgt agccacggta cgctttcagc gcaaacgcgg agatgagcat
    12781 accttccacc agcaggtgcg gcagttgttc catcagcagg cggtctttat aggtgcccgg
    12841 ctccatttca tcggcgttac acagcaggta acggatgttc atggattcgt ctttcggcat
    12901 caggctccat ttcaggccgg tagaaaagcc cgcgccgccg cgccctttca gaccagcgtc
    12961 ttttacctga ttaacgattt cgtccggaga cagcccggtc agcgccttac gcgcgccttc
    13021 gtaaccgttt ttgctgcgat attcgtccag ccacactggc tgtttgtcat cgcgcagacg
    13081 ccaggtcagc ggatgcgttt cgggagtacg gataatgttt ttcatttata ccgctccagc
    13141 agttcaggga tcgcttccgg ggtcagatgc gcgtgagtgt cctcatcgat catcatgttt
    13201 ggccctttgt cgcagttccc caggcagcaa gttggcagca gcgtaaagcg gccatcaaat
    13261 gtcgtttgcc ctggtttgat gttcagcttt ttctccagcg ccgcctgaat accctggtaa
    13321 ccgttgatgt gacagaccac gctgtcgcaa taacggatca catggcgacc aaccggctgg
    13381 cggaagatct ggctatagaa cgttgccacg ccttcgacgt cgctcgccgg aatacccagc
    13441 acatcggcga tcgcgtggat cgcgccatcc ggcacccagc cacgctgctt ctgaacgatt
    13501 ttcagcgctt caatggacgc cgcacgcggg tcttcgtagt ggtgcatctc gtgctcaatc
    13561 gcttcacgct ctgccgcact cagctcaaaa gcctcggttt gtggttgttg attctcgtgc
    13621 ataattagcg gtccacatct gacataacaa aatcgatact gcccagataa acaatcaggt
    13681 cagacaccag gctgccgcgg atcgccgccg gaatttgctg caaatgcgca taactcggcg
    13741 tacggatacg ggtgcgataa ctcatggtgc taccgtcgct ggtcaggtag taactgttga
    13801 tccccttggt cgcctcaatc atctggaaag attcattggc tggcatcacc ggaccccacg
    13861 acacttgcag gaagtgggtg atcagggttt cgatatgttg cagcgtgcgc tctttcggcg
    13921 gcggcgtggt cagcggatga tccgctttga acgggccttc cggcatgttg ttgaggcact
    13981 gctcaagaat gcgcagactc tggcgcagct cttccacttt cagcattacg cgggtgtagc
    14041 agtcagaaac accaccaccc accgggattt caaagtcgaa gttttcatag ccagaataag
    14101 gacgcgcctt acgcacgtcg aagtcgatcc cggtggcgcg caggcccgcg ccagtggtgc
    14161 cccactccag cgcctctttc gcgccatagg cggcaacgcc ctgggaacga cctttcagaa
    14221 tggtgttttg cagcgccgct ttctcgtaag acgccagacg tttcggcatc cagtcgagga
    14281 actcacgcag caggcgatcc cagccacgcg gcaggtcgtg cgctacaccg ccaatacgga
    14341 accacgccgg gtgcatacga aaacccgtga tcgcttccac cagatcgtaa attttctgac
    14401 gatcggtaaa ggcgaagaac actggcgtca tcgcgccgac gtcctgaata aaggtggaga
    14461 tatacagcag gtgactgttg atgcggaaca gttcggagag cattacgcga atgacgttaa
    14521 cgcgatccgg cacggtgatc ccggccagtt tctctaccgc cagcacgtaa ggcatttcgt
    14581 taacgcagcc gccgaggtat tcgatacggt cggtatacgg aatgtagctg tgccaggact
    14641 ggcgttcgcc cattttctcc gcaccacggt ggtggtagcc gatgtctggt acgcagtcga
    14701 caatttcttc gccatcaagc tgcaaaacga tgcggaaagc cccgtgcgcc gacgggtggt
    14761 tcgggccgag gttgaggaac atgaagtcct cgttttcggt gccgcgcttc atcccccact
    14821 cttccggttt gaaggtcagg gcttccatct ccaggtcctg tttggctttg gtcagctcaa
    14881 acggcgagaa ttcggtagcg cgcgccggat aatctttacg cagcgggtga cctttccagg
    14941 tttgcggcat catgatgcgg cgcaggttcg ggtgaccgtc gaaagtaatg ccaaacagat
    15001 cccaggtttc acgctcatac cagttagcgt tcgggaacag tttggtgaag gtcggtacgt
    15061 gcaggtcgtt ttctgccagc gccaccttca gcatgatgtc gcggttacga tcgatagaaa
    15121 tcagatggta gaaaacggaa aaatccgcgg caggtaaccc ttcgcggtgt gtgcgcagac
    15181 gttcgtccat gccgtgtaag tcaaacagca tgacgtaagg tttcggcagt ttctttaaga
    15241 aatcgcccac ttccagtaat tgttcacgct tgatccacac aacgggaacc ccggtgcgag
    15301 tcgcctgaac agtaaaggca tccggcccaa aacggttgcg cagttcgcca atcaccggat
    15361 catcaagatg atcgcgggtc tgccaggcgg gttcttgcgc ggttaagtcg gtcatattgt
    15421 tcaccattgc aaatggtccg tggtgactgt cggcgcagta agcttcgcga aatagattta
    15481 ggaataagcg aagaaaaatt cctttgccga caggcgcaaa ttaaatctcg tcaggtgtac
    15541 gcaggttggt tacggcaatg cgttcaccgc gcttgcgttc gcgctctgat tgcatattgg
    15601 cgcgataaac gccctgatcg ccaaccaccc aggagagcgg acgacgttct ttaccgatag
    15661 attcctgcaa cagcatcagt gcctgcatgt acgcttcagg acgcggaggg cagcccggga
    15721 tatacacatc aaccgggatg aatttatcga cgccctgcac aacggaataa atatcgtaca
    15781 taccgccgga gttggcacag gcacccattg agataaccca ttttggttcc agcatctggt
    15841 catacagacg ctgaataacc ggtgccattt ttgtaaagca ggttcctgca acgaccatca
    15901 ggtcagcctg acgcggcgaa gctcgcaata cttctgcgcc aaaacgcgcc acgtcatgca
    15961 ccgcggtaaa cgaagtcacc atctcaacgt aacagcagga aagaccgaag ttatacggcc
    16021 aaattgagtt tttacgaccc cagttaacca tgtcattgag cttgcccata aacacgtttt
    16081 tattaacttc ttgctccaga gggtcggtta cgatctcctg cttttgcagg gggtaacggt
    16141 cgttctcacc gttgggatct atgcgggtga gcgtataatc catcttaatg cctcgcggtt
    16201 agcgttgacg attagcgata ctgttcgttt ccgggttcat acgctcgcgg cgtgaacgcg
    16261 cgggcgtcca gtccagcgcg ccaatacgca ccagataaac cagacctgcc agtaacacaa
    16321 aaataaaaat tgcagcttcc acaaagccta cccagccgct ttcgcggata gaggttgacc
    16381 atgcgaacag atacagcgct tcaacgtcga agataacgaa gaacatggcc accagataaa
    16441 acttggcgga caggcgtaag cgggcggagc cgaccgagtc gataccggat tcaaacggaa
    16501 cgtttttcga ccttgcgcgt gcgcgaccgc ctaaaaacca accgcctacc agcatcaggc
    16561 aacacaggcc aatggcaacg ataagaaaga tagcgaatgc ccagtgatga gcgatgactt
    16621 cagtggatgt tgacatactc attgcttact catcaaaagt agcgccagat tcactgctct
    16681 tcacggcaga tggacgccac atcgattcat ggggaggaat aaaaaaaacc ttacaatcac
    16741 tgtagaaatt cttttataca gctaattgat gtggtctttt actcctttct ataacctttt
    16801 gtcaacttta acaaaagttt cttcacatta gtttacataa tatcaacacc attagcattt
    16861 aatgcccttt caccccagat ccttgacgac tccaggataa ttagatgttg ttgaatcgtg
    16921 tccgttgtga agcaatggaa aaaatacggg tctattttga caggaatttg tgtcgattcc
    16981 tccccccaaa agagagtatt ttcttgatct gtgacacgct tttgtcattc cataacaaaa
    17041 acgcagcaac aaatttacgt attttttaac atcattgtag caggtgattt ttttcaggcg
    17101 attatttgtg cgttcgggac gtgaatctct ggtgattgaa aaatgaacag ttttgtacgt
    17161 tctgcacgat acgaggaagg ctttttacta aaaaaaagcc gctggggttt aaaacacccc
    17221 cagcggctcg tttttacact attgtctcag aaattatcta tggtccgtcg attactcgat
    17281 atccctttca atcaacaatg agtcatcgcc ttccggagca gacattgggc tgtattgcca
    17341 cggattatgg tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga
    17401 cgggtcatag cacagcaaat attcggtatc gggtagcggc ggtaagccat ctactccact
    17461 cagcacacgc aggtctggac tcatcatctc gaccggcctt gccgtcacgc caagaccggc
    17521 ttttactgcc gcacgaactg ccggaagcgt cgaggcgaca taagccagtc gccatggaat
    17581 atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac gggctaggat catccagcaa
    17641 tacaagaggg atcggctcgc ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt
    17701 tggcgaggta cgcaggttca atgccttaaa cgttgaagga cgatgggtgg ttaccatcaa
    17761 atccacttcc tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc
    17821 cagcgctagt ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc
    17881 ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga acattaatga
    17941 tgagcaggcc tcatcattaa aacgcagaat ttttctggcg taaccaagaa gttgaatgcc
    18001 atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg
    18061 ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc
    18121 ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag
    18181 gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga
    18241 gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca
    18301 gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact
    18361 cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag gaatcgcata
    18421 tctattaagt tactcactct tttgtattta tgacatgcgc gtgtttgtat aaatgtaaat
    18481 gtgagtcctt gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag
    18541 caatggtgag gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa
    18601 aaccttttat ttataagggt cattgcgaat tatctgatg
//
LOCUS       DAZGHF010000091        18546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGHF010000091 DAZGHF010000000
VERSION     DAZGHF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.091"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..580)
                     /gene="rsmG"
                     /locus_tag="ACHK3A_003315"
     CDS             complement(<1..580)
                     /gene="rsmG"
                     /locus_tag="ACHK3A_003315"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO8741361.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLHVPAL"
     gene            complement(644..2533)
                     /gene="mnmG"
                     /locus_tag="ACHK3A_003316"
     CDS             complement(644..2533)
                     /gene="mnmG"
                     /locus_tag="ACHK3A_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO8741362.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2912..3355)
                     /gene="mioC"
                     /locus_tag="ACHK3A_003317"
     CDS             complement(2912..3355)
                     /gene="mioC"
                     /locus_tag="ACHK3A_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO8741363.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3445..3903)
                     /gene="asnC"
                     /locus_tag="ACHK3A_003318"
     CDS             complement(3445..3903)
                     /gene="asnC"
                     /locus_tag="ACHK3A_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO8741364.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4055..5047
                     /gene="asnA"
                     /locus_tag="ACHK3A_003319"
     CDS             4055..5047
                     /gene="asnA"
                     /locus_tag="ACHK3A_003319"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO8741365.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5052..6503)
                     /gene="viaA"
                     /locus_tag="ACHK3A_003320"
     CDS             complement(5052..6503)
                     /gene="viaA"
                     /locus_tag="ACHK3A_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO8741366.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCMALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6497..7993)
                     /gene="ravA"
                     /locus_tag="ACHK3A_003321"
     CDS             complement(6497..7993)
                     /gene="ravA"
                     /locus_tag="ACHK3A_003321"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO8741367.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8216..10084
                     /gene="kup"
                     /locus_tag="ACHK3A_003322"
     CDS             8216..10084
                     /gene="kup"
                     /locus_tag="ACHK3A_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO8741368.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10251..10670
                     /gene="rbsD"
                     /locus_tag="ACHK3A_003323"
     CDS             10251..10670
                     /gene="rbsD"
                     /locus_tag="ACHK3A_003323"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO8741369.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPHLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10678..12183
                     /gene="rbsA"
                     /locus_tag="ACHK3A_003324"
     CDS             10678..12183
                     /gene="rbsA"
                     /locus_tag="ACHK3A_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO8741370.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12188..13153
                     /gene="rbsC"
                     /locus_tag="ACHK3A_003325"
     CDS             12188..13153
                     /gene="rbsC"
                     /locus_tag="ACHK3A_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO8741371.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13178..14068
                     /gene="rbsB"
                     /locus_tag="ACHK3A_003326"
     CDS             13178..14068
                     /gene="rbsB"
                     /locus_tag="ACHK3A_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO8741372.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14194..15123
                     /gene="rbsK"
                     /locus_tag="ACHK3A_003327"
     CDS             14194..15123
                     /gene="rbsK"
                     /locus_tag="ACHK3A_003327"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO8741373.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15127..16119
                     /gene="rbsR"
                     /locus_tag="ACHK3A_003328"
     CDS             15127..16119
                     /gene="rbsR"
                     /locus_tag="ACHK3A_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO8741374.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLLHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16085..17512)
                     /gene="mdtD"
                     /locus_tag="ACHK3A_003329"
     CDS             complement(16085..17512)
                     /gene="mdtD"
                     /locus_tag="ACHK3A_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO8741375.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17535..18227)
                     /gene="yieP"
                     /locus_tag="ACHK3A_003330"
     CDS             complement(17535..18227)
                     /gene="yieP"
                     /locus_tag="ACHK3A_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO8741376.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAMVGTAEQKAHLNTLMTEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4469 a         4613 c         4849 g         4615 t
ORIGIN      
        1 ccagggccgg aacgtgaagt ttaaccactg attcgacctg atattcttcg ggcaacaaag
       61 cgatttcatc ttccggcatt tggcctttca gcgcgtagaa acggccttgc tcaccaggaa
      121 gatggtgaca ccagctcacc atatcgttca gagaggcaaa agcgcggcta attacgccat
      181 caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggctca atattctcca
      241 gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg ctatccaaca
      301 gagtgaaatg ggcttcagga cgcacgatag agagtggaat gccaggcagt cctggaccgg
      361 tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg ctatcgagaa
      421 tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac gctttattcc
      481 atttatgcag catattcaca taggcaataa gctggttttt ctggtgatcg gtaagcgaaa
      541 tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc tgttcttgat
      601 gcgttgcccg gtaagcgggt gcttaccagg catttttaat gcgttatgcg ctacgacgca
      661 gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc gtgacgccag
      721 aaatacgcga agcctggccg atagaggccg gtttgtgatc gttaagtttg gcgatcactt
      781 cgttagaaag accggatacc tggcggtaat ccagcgtcgc tggtagcagg gtgttctcgt
      841 tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg tatttaacct
      901 gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac ggcgtcagcg
      961 tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca ctggcttcac
     1021 gggaaagtgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc gacggggtta
     1081 cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc tcgttaaagc
     1141 gcgcccaacg ttcgtcatcc accaggccca gttcacgacc aatttcagtc aaacgcagat
     1201 ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaggtaaac atacgatacg
     1261 gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatac gcctgagaac
     1321 gcgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga ccggccagca
     1381 aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca gcaaagaaca
     1441 gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgtggatcg aagaagtcat
     1501 actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc atggagcgga
     1561 cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga taaatttcgt
     1621 ttgaggtcag cccttccggt tcaaggaaga tctgatgctg atttctgtcg gcgaagcgca
     1681 tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc acccctgcat
     1741 acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg ttggtatggg
     1801 tgatataaca cggcacctgc tgtggatgct gggacgcatt acccataaac gagaataccg
     1861 gcattgggtt atcgccatgc tgttgcgcca gcacgctgaa gtcgatagta cgcgcatcaa
     1921 tacgcggcgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca cgcaaacggc
     1981 gagagagcgg aatggacggc ggatcaccag cacgaccacc gctgtagtta tccagaccga
     2041 tatgaatttt accgtcgagg aacgtcccaa cggtaagcac gacggctttg gcacggaact
     2101 tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata agatcttcaa
     2161 ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt accgcctgac
     2221 ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtccc ttgcttgcgt
     2281 ttagtatcct aaactggata cccgcctgat cgatcgcttt cgccatcaga ccgccgagtg
     2341 catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg ttgcagctca
     2401 tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc atacgcgccg
     2461 cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg tcaaaaggat
     2521 ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt gaagcccggg
     2581 ccgtggattc tactcaactt tgtcgacttg agaaagacct gggatcctgg gtattaaaaa
     2641 gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat cgcactgccc
     2701 tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca ttaactgtga
     2761 atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat acacaactca
     2821 aaaactgaac aacggttgtt ctttggataa ctaccggttg atccaagctt cctgacagag
     2881 ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc cacgatccca
     2941 gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc ttcagtgttt
     3001 cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg atagccccac
     3061 aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc gcagaaagat
     3121 cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga atatctccgg
     3181 caccgtgggt tgagctgata accagccaga tcccagaggc agataaatct tccaacagcg
     3241 gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc aggtgttctg
     3301 ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct gccataaacc
     3361 gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc tacctgtgga
     3421 aaagtatggg attaaaaaag ccgatcaggg cttgatggta cgcatgatcg ggttttgcag
     3481 gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt tgataagtac
     3541 atgctggaga gcgtcgatcg aacggcacat cacttttata aagatgctgt agtggccggt
     3601 ggtgtaatag gcttcagtga cttcatcaag gctttccagt ttcgccagcg cggaaggata
     3661 gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac cgagctgctt
     3721 cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt tctctactcg
     3781 aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt aagcggtgcg
     3841 cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatta gataattttc
     3901 cataggattt tcttatgcgg attgatgatt cattctattt tagccttctt ttttaatgaa
     3961 tcaaaagtga gttaggcttt ttattgaatg attattgcat gcgtgtcggt ttttgttgct
     4021 taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca ttgccaaaca
     4081 acgtcaaatt agcttcgtga aatctcactt ttcccgtcaa ctggaagaac gtctggggct
     4141 gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg ataacttgtc
     4201 gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc agttcgaagt
     4261 ggttcattca ctggcgaagt ggaaacgtca gacattaggg caacacgact tcagcgcggg
     4321 cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc tttctccgtt
     4381 gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg gtgagcgtca
     4441 attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag caaccgaagc
     4501 tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc acttcgtaca
     4561 cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg agcgggcgat
     4621 tgcgaaagat cttggcgcgg tattcctcgt cgggattggc ggcaagttga gtgatggtca
     4681 tcgtcacgac gtgcgcgcac cggattatga tgactggagc accccgtcag agctgggcca
     4741 tgcgggtctg aacggcgata ttctggtatg gaacccggta ctggaagatg cgtttgagct
     4801 ttcttccatg gggatccgcg tggatgccga tacgttgaaa catcagctgg cgctgaccgg
     4861 tgatgaagat cgcctggagc tggagtggca tcaggcgctg ctgcgcggtg aaatgccgca
     4921 gaccatcggc ggcggtatcg ggcagtctcg tttgaccatg ctgctgctgc aactgccaca
     4981 tatcggccag gttcagtgtg gtgtatggcc ggcagctgtt cgcgagagcg tcccttctct
     5041 gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc ggtatcaaag
     5101 cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc catgtgcaga catcgccacg
     5161 gcatgaaagc gatgctgatg tacgcgctgt agctctttca ctttactcgt cacgtcgtca
     5221 ggcaaccgct gggcgataaa atcagaaatc accaccgcat cggcatcaaa ccattccctg
     5281 ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc gccacgaaac
     5341 tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag ctcataacgg
     5401 acgatctcgg tggaaaataa cataatatag cagcgccggt tttcagcgag agcaatgcgc
     5461 atcaaggcca tgcagaacgc tttcgcacac tgttcattaa agccgcccat tgagccggaa
     5521 gtatccacac agacaataaa cggtccgcgc ggctgttcgt cgtaatcttt atgtaccacc
     5581 gggcgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt gagcaactgt
     5641 ttttccacca gccgacggta aaactcatac tccagttccg ttatccccag tgtcgccagt
     5701 tctggcggca ggagacgtaa aatatcatcg ctttgttgca gaccatcaac ctgctcagga
     5761 accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc gttgcgcggt
     5821 attgatttgg cttcccgtga acgccccaac tgttctgcca ggcgtttcag ttccggctgt
     5881 tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccgcgttt aagctgaccg
     5941 gcgctcatat cccacagacg accagctgcg gtattgttat ctgcgagaat cggttcaagt
     6001 tgcccgctca gcgtcatgcg ttcctgaact tcactcaaca gttgttcgcg ttcttcttct
     6061 aatagctgct gatttagcgt cgttgcttgc acgatcagac ttaaacgcca gcgctggaga
     6121 aaaagcgtgt gtaacgctga agtgatcgta ctgttagcat caaccaactg tcgggcttgt
     6181 tctgcccatg gagaattcag acgatgcagt aagtccagga tctgtggtag ctgcacaata
     6241 aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc ggtgagttcc
     6301 ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg gggaacatca
     6361 tcagtgattg ccgccttcag tcgtgggaat ttttcaaaga agaccgccag ctgcggtgag
     6421 gccagcagcg cgatgatcat ctcttcgatc aatccctctt cgctgacggc cagcatcaca
     6481 ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgggcgccg acatcctgta
     6541 ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt tgctgttcgc
     6601 tgaataaagc gtgttgcttg cgccagtcgc tttcaagctc ttccagttgt tgttttattt
     6661 cacctggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct tgtaaactta
     6721 catcgcgtac aacaagatgt tgggcgctat ccacttccag attcaatttc tgggcaaagc
     6781 cgataccgtt cagtttgccg cgaatttcac cacctttact cagccactgt tccagcgcgt
     6841 tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt ttttgcagca
     6901 gcagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga cgacggctga
     6961 aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc tgctggagtt
     7021 gcaggtgacg ttgcacaatc gcgcccaggc gggtcaacat tccttgctgt tgccaggcat
     7081 gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc gcgtcatacc
     7141 acaggcaatc tttcagcaaa atgagatcaa ccggggcaac agcactgcga ccgctaaaaa
     7201 aggcgctggc ctgcaataat cgaatcgctt ttttccagcg acgatccgag acataaggcg
     7261 catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat acatgatcgg
     7321 gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct gtgacctgca
     7381 aggaggcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg gagcggaaat
     7441 tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca tataacgctt
     7501 ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcagg cgcatcggga
     7561 ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg agcagggtat
     7621 taagaattgc cggacccgct ttccagatct catccagaaa gacgatttct gcttccggca
     7681 ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga atagaaaggg
     7741 gaccaaaaac ctcttccggc gtggagaagc gagtcatcag atattcaaac gcgcgggcat
     7801 tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct ggcgggccaa
     7861 ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg gcgtggctac
     7921 gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc gctaataaat
     7981 gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa attgcgagta
     8041 tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg gggtattttt ctttgagcca
     8101 ggttaatgtg cccgcattta ggagtacgat tttgccgtta atcgtgcata ctgtgcgctt
     8161 ttttgtgggc caagggacta agcacacatt tcatatttca acgaaagact agtctatgag
     8221 cactgataat aagcaatcat tgcccgcgat taccctcgcg gcgattggag ttgtctacgg
     8281 cgatattggt accagcccgt tatatacact tcgtgaatgt ttgtccggcc agtttggttt
     8341 tggcgttgag cgcgatgccg tgtttggctt tttatcgctg atcttctggc tgctaatctt
     8401 tgtggtttct attaaatatc tcaccttcgt gatgcgggca gataacgccg gtgaaggggg
     8461 gatcctgacg ttgatgtcgc ttgccgggcg taatacgtcg gcgcgaacca catcaatgct
     8521 ggtgattatg gggctaatcg gcggcagctt tttctatggt gaagtcgtca taacacccgc
     8581 tatttcggtg atgtccgcca ttgaaggtct ggaaatcgtc gccccgcagc tggatacctg
     8641 gatagttccc ctctcaatta tcgttctcac tttattattt atgattcaaa agcatggcac
     8701 ggctatggtc ggtaagctgt ttgcgccgat catgctgacc tggtttttga ttctggcagg
     8761 actggggtta cgtagcatta ttgctaaccc ggaagtgctg catgcgctga atccgatgtg
     8821 ggcggtgcat ttcttccttg aatacaaaac ggtttctttt attgcattag gggcagtggt
     8881 gctgtcgatt acgggggtcg aggcgctgta tgctgatatg gggcactttg gtaagttccc
     8941 tattcgcctg gcgtggttca ccgtcgtatt accttccttg acccttaatt acttcggtca
     9001 gggagcgttg ttgttaaaga acccggaagc gattaagaac ccgttcttcc tgttggcacc
     9061 ggactgggcg ctgatcccgc tgctgatcat cgccgcactg gcgacggtaa ttgcctcgca
     9121 ggctgttatc tctggcgtct tctcattgac gcgtcaggcg gtacgtctgg gatatttgtc
     9181 gccgatgcgc attattcaca cctccgaaat ggagtcaggg caaatctata ttccctttgt
     9241 gaactggatg ctctatgtcg cggtcgtgat tgtgattgtc agctttgagc actccagcaa
     9301 cctggcggcg gcgtacggga ttgcggtgac cggaaccatg gtgctgacgt ctattctctc
     9361 gactaccgtg gcacgtcaga actggcactg gaataagtat tttgttgcgc tgatcctgat
     9421 tgctttcctt tgtgtcgata ttccattgtt caccgctaac ctcgataaac tgctctccgg
     9481 cggctggttg ccattgagcc tcggtacggt gatgtttatc gtgatgacca cctggaagag
     9541 cgaacgtttc cgtttgctgc ggcggatgca tgaacatggt aactctctgg aggcgatgat
     9601 tgcttcgctg gagaaatcac cgcccgttcg cgtgcccggg accgcggtgt atatgtcacg
     9661 tgcaatcaac gtcattccct ttgcgctgat gcataacctt aaacataaca aggtattgca
     9721 tgagcgggtg attctgttaa ctctgcgcac cgaagacgcg ccatatgtcc ataacgtccg
     9781 tcgggtacag attgaacaac tgtcgcccac tttctggcgc gtggtggcaa gttatggttg
     9841 gcgagaaacg ccaaacgtgg aagaagtttt ccaccgctgc ggtctggaag gattaagttg
     9901 ccggatgatg gaaacctcct tctttatgtc gcatgagtcg ttgatcctcg gcaaacgccc
     9961 gtggtatttg cgtctgcgcg gcaagctgta cttgctgctg caacgtaatg cgctgcgtgc
    10021 gccggatcaa tttgaaatcc cgccaaacag ggttatcgaa ctgggtactc aggtcgaaat
    10081 ctaacgccag acgcctcctt tctccttaag ggggcgtttc tgttttcatg gttaattacc
    10141 atgtaaaacg tttcgaggtt gatcacattt ccgtaacgtc acgatggttt tcccaactca
    10201 gtcaggatta aactgcgggt cagcgaaacg tttcgctgat ggagaaaaaa atgaaaaaag
    10261 gcaccgttct taattctgat atttcatcgg tgatctcccg tctgggacat accgatacgc
    10321 tggtggtgtg tgatgctggt ttacccatcc ccaaaagtac aacgcgtatc gatatggcat
    10381 taacccaggg cgtaccttct tttatgcagg tgctgggcgt cgtcacaaat gaaatgcagg
    10441 tcgaggcagc cattatcgcg gaagagatca aacaacataa tccgcatctc cacgaaacgt
    10501 tgctcactca ccttgagcag ctgcaaaaac accagggaaa taccattgaa attcgttaca
    10561 ccacgcatga acaattcaaa caacaaaccg cagaaagtca ggcggtaatt cgcagcggag
    10621 aatgttctcc gtatgcgaat atcattctct gtgctggcgt gacgttctga ggccgtcatg
    10681 gaagcattac ttcagcttaa aggcatcgat aaagccttcc cgggcgtaaa agccctctcg
    10741 ggcgcagcgt taaatgtcta tcctggccgc gtgatggcgc tggtgggcga aaacggtgcg
    10801 ggtaaatcca ccatgatgaa agtgcttacg ggtatctata ctcgcgatgc cggtacgctt
    10861 ttatggctgg ggaaagaaac gacgtttacc gggccgaaat cttcccagga agccgggatt
    10921 gggattatcc atcaggaact gaacctgatc ccgcagttga ccattgccga aaacattttc
    10981 ctcggtcgtg agtttgttaa tcgctttggc aaaattgact ggaaaaccat gtatgccgaa
    11041 gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagct ggtgggcgat
    11101 ctttccatcg gtgaccagca aatggttgaa attgccaaag tgctgagctt tgagtcgaaa
    11161 gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga atccttgttc
    11221 cgcgtcatcc gcgagctgaa atcgcaaggc cgcggtattg tctatatctc ccaccgcatg
    11281 aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca atttattgct
    11341 gagcgtgaag tagcatcact gaccgaagat tcgctgattg agatgatggt gggtcgcaag
    11401 ctggaagatc aatatccgca cctggacaaa gcgccgggag atatccgcct gaaagtcgat
    11461 aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg cgaaattctt
    11521 ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct ctacggcgca
    11581 ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac ccgttcaccg
    11641 caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg tgacggttta
    11701 gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta cttcagccgc
    11761 gctggcggca gtttgaagca tgccgatgaa cagcaggcag taagtgattt cattcgtctg
    11821 tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctctccgg tggcaatcag
    11881 caaaaagtgg cgattgcccg tggtctgatg acacgcccca aagtgttgat cctcgatgag
    11941 cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat taaccagttc
    12001 aaagccgatg gcttgagcat cattctggtg tcatcggaga tgccagaagt attaggcatg
    12061 agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac tcgtgagcag
    12121 gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt gaatcaggag
    12181 taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag cgtggctgat
    12241 ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga cgttaagccc
    12301 gaactttttc accatcaata acttattcaa tattctccag caaacctcgg tgaacgccat
    12361 tatggcagtc gggatgacgc tggtgatcct gacgtcgggc atcgacttgt cggtaggttc
    12421 tctattggcg ctgaccggcg cagttgctgc atctatcgtt ggcattgaag tcaatgcgct
    12481 ggtggctgtc gctgccgctc tcgcgttagg tgccgcaatt ggtgcggtaa ccggggtgat
    12541 tgtagcgaaa ggtcgcgtcc aggcctttat cgctacgctg gttatgatgc ttttactgcg
    12601 cggcgtgacc atggtttata ccaacggtag cccagtgaat actggcttta ctgagaacgc
    12661 cgatctgttt ggctggtttg gtattggtcg tccgctgggt gtaccgacgc cagtctggat
    12721 catgggaatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc tggggcgtta
    12781 catctacgcg ctgggcggca acgaagcggc aacgcgcctt tctggtatca acgtcaataa
    12841 aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg ggatcattga
    12901 agtggcgcgt ctctcctccg cacaacctac ggcggggact ggctatgagc tggatgccat
    12961 tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca ttgttgggac
    13021 gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt taggtgtttc
    13081 ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc tggtagacaa
    13141 caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa aactggctac
    13201 cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg caaaagacac
    13261 catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga aagatggcgc
    13321 gcagaaagag gcggataaac ttggctataa cctggtggtg ctggactccc agaataaccc
    13381 ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa ttctgctgat
    13441 taacccgacc gactccgacg cagtgggtaa tgctgtgaag atggctaacc aggcgaacat
    13501 cccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc acattgcttc
    13561 tgataacgta ctgggcggca aaatcgccgg tgattacatc gcgaagaaag cgggtgaagg
    13621 tgccaaagtt atcgagctgc aaggcattgc cggtacatcc gcagcccgtg aacgtggcga
    13681 aggcttccag caggctgttg ctgctcacaa gtttaatgtt cttgccagcc agccagcaga
    13741 ttttgatcgc actaaaggtt tgaacgtaat gcagaacctg ttgaccgcac atccggatgt
    13801 tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcgcg cactgcaaac
    13861 tgccggtaaa tcggatgtga tggtcgtcgg atttgacggt acaccggatg gcgaaaaagc
    13921 ggtgaatgat ggcaaactag cagcgactat cgctcagcta cccgatcaga ttggcgcgaa
    13981 aggcgtcgaa accgccgata aagtactgaa aggtgagaaa gttcaggcta agtatccggt
    14041 tgatctgaaa ctggttgtta agcagtagtt ttaatcaggt tgtatgacct gatggtgaca
    14101 taaatacggc atcgacagat gaacgtgtaa tataaagaaa agcagggcac gcgccaccct
    14161 aacacggtgg cgcattttat ggacatcccg aatatgcaaa acgcaggcag cctcgttgtt
    14221 cttggcagca ttaatgctga ccacattctt aatcttcaat cttttcctac cccaggcgaa
    14281 accgtaaccg gtaaccacta tcaggttgca tttggcggca aaggcgctaa tcaggctgtg
    14341 gctgctgggc gtagcggtgc gaatatcgcg tttattgcct gcactggcga tgacagcatt
    14401 ggtgagagcg ttcgccagca gctcgccact gataacatcg atatttctcc ggtcagcgtg
    14461 atcaaaggcg aatcaacagg tgtggcgctg atttttgtta atggcgaagg tgagaatgtc
    14521 atcggtattc atgccggcgc taatgctgcc ctttccccgg cactggtgga agcgcaacgt
    14581 gagcgtattg ccaacgcgtc agcattatta atgcagctgg aatcaccact cgaaagtgtg
    14641 atggcagcgg cgaaaatcgc ccatcaaaat aagactatcg ttgcgcttaa cccggctccg
    14701 gctcgcgaac ttcctgacga actgctggca ctggtggaca ttattacgcc aaacgaaacg
    14761 gaagcagaaa agctcaccgg tattcgtgtt gaaaatgatg aagatgcagc gaaggcggcg
    14821 caggtactgc atgaaaaagg tatccgtact gtactgatta ctttaggaag tcgtggtgta
    14881 tgggctagcg tgaatggtga aggtcagcgc gttcctggat tccgggtgca ggctgtcgat
    14941 accattgctg ccggagatac ctttaacggc gcgttaatca cggcgttgct ggaagaaaaa
    15001 ccattgccag aggcgattcg gtttgctcat gctgccgctg cgattgccgt aacacgtaaa
    15061 ggcgcacaac cttccgtacc gtggcgtgaa gagatcgacg catttttaga caggcagagg
    15121 tgacgcttgg ctacaatgaa agatgttgcc cgcctggcgg gcgtttctac ctcaacagtt
    15181 tctcacgtta tcaataaaga tcgcttcgtc agtgaagcga ttaccgccaa agttgaagcg
    15241 gcgattaaag aactcaatta cgcgccatca gccctggcgc gtagcctcaa actcaatcaa
    15301 acacatacca ttggcatgtt gatcactgcc agtaccaatc ctttctattc tgaactggtg
    15361 cgtggcgttg aacgcagttg cttcgaacgc ggttatagtc ttgtcctttg caataccgaa
    15421 ggcgatgaac agcggatgaa tcgcaatctg gaaacgctga tgcaaaaacg cgttgatggc
    15481 ttgctgttac tgtgcaccga aacgcatcaa ccttcgcgtg aaatcatgca acgttatccg
    15541 acagtgccta ctgtgatgat ggactgggct ccgttcgatg gcgacagcga tcttattcag
    15601 gataactcgt tgctgggcgg agacttagca acgcaatatc tgatcgataa aggtcatacc
    15661 cgtatcgcct gtattaccgg cccgctggat aaaactccgg cacgcctgcg gttggaaggt
    15721 tatcgggcgg cgatgaaacg tgcgggtctc aacattcctg atggctatga agtcactggt
    15781 gattttgaat ttaacggcgg gtttgacgct atgcgccaac tgttattaca tccgctgcgt
    15841 cctcaggccg tctttaccgg aaatgacgct atggctgttg gcgtttacca ggcgctgtat
    15901 caggcagagt tacaagttcc gcaggatatt gcggtgattg gctatgacga tatcgaactg
    15961 gcaagcttta tgacgccacc attaaccact atccaccaac cgaaagatga actaggggag
    16021 ctggcgattg atgtactcat ccatcggata acccaaccga cccttcagca acaacgatta
    16081 caacttactc cgattctgat ggaacgcggt tcggcttaga tttacgctgt cttttgatca
    16141 aattattacc atcggttgtt ttcagaagca tgaacattgc tgctgaagca acagtaataa
    16201 tgcccatcgt gataaacgta tagtggaatt gttcgacagt cgttgtgcct tccatccctt
    16261 cataaacgcg aaggacggcc gcacttacag caacgcctaa actgatagac agttgctgcg
    16321 tgaccgccag aacactgtta ccgctgctgg cgttgtcatc ggtcagatcg gcaagtgtga
    16381 tggtattcat cgcggtaaat tgcgtcgaca tagccatccc taatataaac aacggcaaga
    16441 tcagcatcca tatcgccatt gccggtgatt gcaaagagaa ctgagcgatc attagcccaa
    16501 taatcaccgt gatccccact aatgtatggc gatagcccag acgacgtaag acttgggtaa
    16561 ccatcgattt tgcaataatg gaacctaacg ctgtcggtgc catcatacag ccggcaataa
    16621 acgcctgata accaaatcct acctgtaaca tcaatggcat aaggaacggt acacaaccgg
    16681 tccccagacg ggttgcaata ttgcctacga taccgatcga gaaagtgcgg gttttaaata
    16741 aatctaatga aattaatggg tttggcgtgc gtcgtgcatg gagaatatag agaagcagta
    16801 acccgatgct ggtgacaatt accgtcaagg caatccaact ggcgacaatc ttttccccga
    16861 atagctctat tccgcttgag aagagaacaa ggctaaggcc aaacagcaaa aagccagtga
    16921 tatcgaatct gcgtcgtgcg gtggtgaaat tgggcatatg tttgcgcgcg taaagaaggc
    16981 ccgcaatacc tatggggata ttgattaaaa atatccagtg ccaggttgcc caggtgacca
    17041 gcacgccgcc aagaacaggg cctaaaattg gccccaccag acccggcatg gcgacaaaat
    17101 tcaatacagg aagaagctca ttacgaggat aagcgcgcag taaggccagc cgagcaacag
    17161 gcatcatcat tgcgccgcct atcccctgaa taacccggaa gacaaccagc tgtggtagcg
    17221 agttagaaag tgcgcaggcc agagaaccca acgtgaacag actcacggca agagtaaata
    17281 tgcgacgcgt accgaagcga tcggctagcc atccgcttac cggaataagc atcgccaccg
    17341 tcagcgtata actgatgatg gctgattgca tcgcgagagg agaacgatta aggctatgag
    17401 cgattgcggg taaggcggta ttcagaatag tggcatcaag tgcctgcata aagaaggcca
    17461 tcgccgcgat ccacggcaaa cccgccatac tgcgcttctt tttatcgctc attcaatgtc
    17521 ctgttatcgg ggtgtcactt atcaggtgag cgtagcagcg cctgacaagc tttaaatgcc
    17581 gcgtcgccat cgctttggat aatcgcgtca acaatcgcct ggtgcagatc cagctttatc
    17641 actgtgtcgc tggtaattga cgtgaagtaa gtgtgataaa ccgaatggaa gagcgaggcg
    17701 aatgaggtca aaaacggatt ggcgctcatt tcatagatat gctcatgcca ggccatatcg
    17761 acctcaatcc agcgttcacg gcgaaagttc tcttttaatg ccaccatttc ggtcattaac
    17821 gtattgagat gcgccttctg ttccgcggtg ccaaccattg ctgccagtag gcaagcttgc
    17881 ggctccagac agatacgcat aaccagaaag tgatcgatga cctgatgaaa gttctcttct
    17941 gtcatccacc aggtaagcaa ttcctgatca agaaaattcc agtttgattg tggcatgacc
    18001 cgggtaccaa ttcgcggtcg cggtaaaacc atcccttttg ccgttaacgt tttgaccgct
    18061 tcgcgtaccg ctgtacgact cactccaaat tgctcgccca gctcaatctc accaggcaaa
    18121 atggtgccgg gttcatattc accttttaag atgcgttgcg ccagcttctc agccagaaca
    18181 tacgaaaggt ttttctgtgc agctaactgt tgtgcgctta aaggcattac taatcttcct
    18241 ttttcttttt attcctcctt agtatgccac caggaagtgt gattacggtt gcaaaaacgg
    18301 caaattgctt gttttatggc acattaacgg ggcttttgct gaaaaaatgc gcggtcagaa
    18361 aattatttta aatttcctct tgtcaggccg gaataactcc ctataatgcg ccaccactga
    18421 cacggaacaa cggcaaacac gccgccgggt tagcggggtt ctcctgagaa ttccggcaga
    18481 gaaagcaaaa acaaatgctt gactctgtag cgggaaagcg tattatgcac accccgcgcc
    18541 gctgag
//
LOCUS       DAZGHF010000092        18413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGHF010000092 DAZGHF010000000
VERSION     DAZGHF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.092"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..83
                     /locus_tag="ACHK3A_003331"
     CDS             <1..83
                     /locus_tag="ACHK3A_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741377.1"
                     /translation="LYISYNKASLNNLVFSRFIDRLNDSF"
     gene            230..1450
                     /gene="ybdN"
                     /locus_tag="ACHK3A_003332"
     CDS             230..1450
                     /gene="ybdN"
                     /locus_tag="ACHK3A_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO8741378.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDMPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1423..2052
                     /gene="ybdM"
                     /locus_tag="ACHK3A_003333"
     CDS             1423..2052
                     /gene="ybdM"
                     /locus_tag="ACHK3A_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO8741379.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLIPNDYNPNNVAPPEKKLLKKSIETDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2053..3213)
                     /gene="ybdL"
                     /locus_tag="ACHK3A_003334"
     CDS             complement(2053..3213)
                     /gene="ybdL"
                     /locus_tag="ACHK3A_003334"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO8741380.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALSESRLEILPCEGTYFLLVDYSAVSSLDD
                     VKFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3322..4410
                     /gene="hcxA"
                     /locus_tag="ACHK3A_003335"
     CDS             3322..4410
                     /gene="hcxA"
                     /locus_tag="ACHK3A_003335"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO8741381.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQKQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTEAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFENVESFKA"
     gene            complement(4420..4617)
                     /gene="ybdD"
                     /locus_tag="ACHK3A_003336"
     CDS             complement(4420..4617)
                     /gene="ybdD"
                     /locus_tag="ACHK3A_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO8741382.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4730..6835)
                     /gene="cstA"
                     /locus_tag="ACHK3A_003337"
     CDS             complement(4730..6835)
                     /gene="cstA"
                     /locus_tag="ACHK3A_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO8741383.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALVALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7016..7429)
                     /gene="entH"
                     /locus_tag="ACHK3A_003338"
     CDS             complement(7016..7429)
                     /gene="entH"
                     /locus_tag="ACHK3A_003338"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO8741384.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7432..8178)
                     /gene="entA"
                     /locus_tag="ACHK3A_003339"
     CDS             complement(7432..8178)
                     /gene="entA"
                     /locus_tag="ACHK3A_003339"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO8741385.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8178..9035)
                     /gene="entB"
                     /locus_tag="ACHK3A_003340"
     CDS             complement(8178..9035)
                     /gene="entB"
                     /locus_tag="ACHK3A_003340"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO8741386.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDSLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9049..10659)
                     /gene="entE"
                     /locus_tag="ACHK3A_003341"
     CDS             complement(9049..10659)
                     /gene="entE"
                     /locus_tag="ACHK3A_003341"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO8741387.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10669..11844)
                     /gene="entC"
                     /locus_tag="ACHK3A_003342"
     CDS             complement(10669..11844)
                     /gene="entC"
                     /locus_tag="ACHK3A_003342"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO8741388.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFAGAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12033..12989
                     /gene="fepB"
                     /locus_tag="ACHK3A_003343"
     CDS             12033..12989
                     /gene="fepB"
                     /locus_tag="ACHK3A_003343"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO8741389.1"
                     /translation="MRLVPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAETVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLASGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12993..14243)
                     /gene="entS"
                     /locus_tag="ACHK3A_003344"
     CDS             complement(12993..14243)
                     /gene="entS"
                     /locus_tag="ACHK3A_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO8741390.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QIMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14354..15358
                     /gene="fepD"
                     /locus_tag="ACHK3A_003345"
     CDS             14354..15358
                     /gene="fepD"
                     /locus_tag="ACHK3A_003345"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO8741391.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLYTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15355..16347
                     /gene="fepG"
                     /locus_tag="ACHK3A_003346"
     CDS             15355..16347
                     /gene="fepG"
                     /locus_tag="ACHK3A_003346"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO8741392.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAITLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAASLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQLLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16344..17159
                     /gene="fepC"
                     /locus_tag="ACHK3A_003347"
     CDS             16344..17159
                     /gene="fepC"
                     /locus_tag="ACHK3A_003347"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO8741393.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTTPSTAKI"
     gene            complement(17156..18289)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK3A_003348"
     CDS             complement(17156..18289)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK3A_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO8741394.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEVDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQALEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GPGKAIIVILSALIGGIVACGGVLLRHAMASRKQDAIMADHLV"
BASE COUNT         4363 a         5193 c         4716 g         4141 t
ORIGIN      
        1 aactttatat ttcctataac aaggcttcgt tgaacaacct tgtcttctcc cgattcatag
       61 atcgtttaaa tgatagcttc tgattcatta ccactgacaa ataatatatt acgcatcaat
      121 taattcattg tgtgaatata aaataatgtg ctctatcttg cttcgtttat cctataatta
      181 gcggattcat ctcacgagtc accccgtaac gttcagagag cgtttttaga tgtctattta
      241 taaaatcccg cttccactga atattctgga agctgcaaaa gaacgtatta cctggacact
      301 taatacccta ccccgtatct gtgtttcttt ttcgggtggt aaagattccg gattaatgct
      361 gcatctgaca gccgaaattg cccgacaaat gggcaaaaaa atctgcgttt tgtttatcga
      421 ctgggaagcg cagttctcat gcactattaa ctatgttcag tcattacgtg agttttacgc
      481 cgatgtcatc gaagagtttt actgggttgc gctcccgctt acgacgcaaa attccctttc
      541 tcaataccaa cccgaatggc agtgctggga acctgatgtc gaatgggtgc gtcagccccc
      601 gcaagatgcg ataaccgatc ctgacttttt ctccttttac cagcctggca tgaccttcga
      661 acaatttgtg cgtgagtttg ctgaatggtt ttcacaaaaa cgtccggcgg cgatgatgat
      721 cggcatccgt gcagatgagt cctacaaccg ttttgtcgcc atcgccagtt taaataaaca
      781 acgttttgcc gatgatatgc cctggaccac cgccgcacca ggcggtcata gctggtacat
      841 ttaccccatc tacgactgga aagtggctga tatctggacc tggtatgcta accatcaaca
      901 gctctgtaac ccactgtata atataatgta tcaggcaggc gttcctctgc gtcatatgcg
      961 aatttgcgaa ccttttggcc cggagcaacg gcaaggatta tggctctatc acgttatcga
     1021 accggatcgc tgggctgcta tgtgcgcacg agtcagcggg gtaaaaagtg gcggcattta
     1081 cgccggacat gataatcatt tttatggtca tcggaaaatc ctcaagccag aacatttaga
     1141 ctggcaagaa tatgcattat tgctgctcaa tagcatgccg gaaaaaacag ctgagcatta
     1201 ccgcaataaa atcgccattt atttgcactg gtatcagaaa aaaggcatcg aagttccaca
     1261 aacccagcaa ggggacattg gcgcgaaaga tatcccctcc tggcggcgaa tatgcaaagt
     1321 tttactcaat aacgattact ggtgccgggc attatcattt agtcctacga aagcgaagaa
     1381 ctatcagcgt tataacgaac ggataaaagg aaaacgtcag gaatggggga tactatgcaa
     1441 caacgattaa cgcaggattt aacccaattt ctcgccagct tgccggaaga tgatcgcatc
     1501 aaagcgatta atgaaatccg catggcgatc catcaggtta gcccctttcg tgaagagcca
     1561 gtggattgcg ttctgtgggt taaaaacagc caacttatac ctaatgatta caacccaaac
     1621 aacgtggcac cacccgaaaa aaaactgctg aaaaaatcca ttgaaacaga tggttttacc
     1681 caacccattg tggtcacgca tacggataaa aatgccctgg aaatcgtgga tggttttcac
     1741 cgccatgaaa ttggtaaagg ctcatcatcg ttaaaattac gcttaaaagg ttatttgccg
     1801 gtgacctgtc tggaaggtac gcgtaatcaa cgcatcgccg cgactattcg tcataaccgc
     1861 gctcgtggtc gccaccaaat cactgccatg tccgaaattg ttcgtgaact cagccagttg
     1921 ggatgggacg ataataaaat tggcaaagag ctgggaatgg acagcgacga agtgctgcgc
     1981 ctgaaacaaa ttaacggcct gcaagagttg tttgccgacc gtcaatattc ccgtgcctgg
     2041 actgtcaaat aactacagct ggcgcaggcg ttcagctgct gccagcaacg tcgattcctt
     2101 cttggcaaaa cagagacgaa tcagtttatg ggggaaggga tcggcgcaaa ataccgacag
     2161 cgggatcgcc gctacaccgt gctcccgcgt cagccactgg caaaacttaa cgtcatctag
     2221 cgaagaaacc gcgctgtaat ccaccagcaa aaagtatgta ccttcgcacg gtaaaatctc
     2281 cagccgactc tcacttaagg cattcaccag aatatcgcgt ttctggcgat aaaagtccgg
     2341 taacgcaaga taatgctcag gttctgcacg tagcatatcg gcaagcgcca actgtgccgg
     2401 ggtattcacc gaaaaggtca gatactgatg cactttgcgg atctcggcgc tgatgggcgc
     2461 aggcgcaacg caataaccca ctttccagcc ggtcatatga taggtcttgc caaacgatga
     2521 caccgccacc gctcgctcac gcaattgcgg atgcgccagc acgctggcat ggccctgttg
     2581 tgaaaaattg atgtgctcgt agacttcatc gctaatgaca aaaatctcgt gcccggcgat
     2641 cgcctgccac aaagcggcga aatcagcctg ctgccagaca gttgcactgg ggttatgcgg
     2701 ggtgttgagg atcaccagtc gggtgcgctc gcttaacaat gcggcaaatt cctgccagtc
     2761 cacgcgaaaa tgcggtggtt gcagtgcaat acgtttcact attccccccg aaagcgcgat
     2821 ggcgggcgcg taactgtcat agctgggatc aaagcagatc acttcatcac cattgcgcac
     2881 caatgcggta atcgctgcat ataacgcttc cgtcgcccct gccgttacgg tgatatcgct
     2941 atcggcatcc ggttgatagc catacagacg ttccgttttc tgagcaatcg cctcgcgcaa
     3001 ggcctgcacg ccggtcatgg gcgcgtattg gtttgccccc tgggcaacgt ggtacgccag
     3061 ccgctcctgt aaatagcgcg gaccatcaaa atcaggaaag ccttgtgaaa ggttaatcgc
     3121 ctggtgttgc tgcgccagcg cgctcatccg ggtgaaaata gtggtgccaa gttgtggaag
     3181 tttgctttgt ggaatcagag ggttatttgt catttttatt gtacctgacg aagttgcgtg
     3241 ttgaaggcac tataacacga tgctagtatt tggcaatcaa gacgtttaga tgtctaaata
     3301 aaacaataag gacaacacaa catgcctcac aatcctatcc gcgtggtcgt cggcccggct
     3361 aactactttt cacatcccgg aagtttcaat cacctgcacg attttttcac tgatgaacaa
     3421 ctttctcgcg cggtgtggat ctacggcgaa cgcgccattg ctgcggcaca aaccaaactg
     3481 ccgcctgcgt ttgaactgcc aggggtaaag catattttgt ttcgtggtca ttgcagcgaa
     3541 agcgatgtgc aacaactggc ggcggagtcc ggtgacgatc gcagcgtggt gattggcgtc
     3601 ggcggcggcg cactactcga caccgcgaaa gccctcgccc gccgtctcgg tctgccgttt
     3661 gttgccgttc cgacgatcgc cgccacctgc gccgcctgga caccgctctc cgtctggtat
     3721 aacgatgccg gacaggcgct gcattatgag attttcgacg acgccaattt tatggtgctg
     3781 gtggagccag agattatcct caatgcgccg caagaatatc tgctggcggg gatcggtgac
     3841 acgctggcga aatggtatga agcggtggtg ctggctccgc aaccagaaac gttgccgcta
     3901 accgtgcggc tggggatcaa taatgcgcag gccattcgcg acgtcttgtt aaacagtagc
     3961 gaacaggcgc tgagcgatca gcaaaaacaa cagttaacgc aatcattttg cgatgtggtg
     4021 gatgctatta ttgctggtgg tgggatggtc ggtggtctgg gcgatcgttt tacgcgtgtg
     4081 gcggcggccc atgccgtgca taacggtctg accgttttgc cgcaaaccga gaagtttctc
     4141 cacggcacca aagtcgccta cggaattctg gtgcaaagcg ccttgctggg tcaggatgat
     4201 gtgctggcgc aattaacaga ggcgtatcag cgttttcatc tgccgactac gctggcggag
     4261 ctggaagtgg atatcaataa tcaggtggag atcgacaaaa tgattgccca caccctacgt
     4321 ccggtggaat ccattcatta cctgccagtc acgctgacac cagatacgtt gcgtgcagcg
     4381 ttcgaaaacg tggaatcgtt taaagcctga cgtacaaatt tagcagcagc gcgcgccgcc
     4441 ttttccaccg tagcgcgcgt cctgccgctc ccggaaaaac tcctcgtagg tcatcggtgt
     4501 ttgatcggga tggttaaccc gcatatgttc gacatagttg tcgtaatcag gcataccaat
     4561 catcagtttc gccgcctgac ctaaatattt tccggctttt gccagtgaat caaacatcta
     4621 aacctccggt aatacaaatg ccctcctgaa ttaagagggc ttaattgcta tcaacacgct
     4681 cgggatgaga gtaaggggaa cgctcagcac attccccctc actctgactt tagtgcgcgc
     4741 ctttcgcctg cgccacgatc tcctcgacat tttccggcat cggctcatat ggcgtttctt
     4801 tcgccgttgg cttcggctct ttcaatgcca ccagtgccgt cttaatcgag aacagcgcca
     4861 gaaccaccac aaccaccata aagaagatgg ttaatccggc atccagacgg ttgttgaaca
     4921 ccagttgcgc cagttgtgac tcagtatact gcgacggaat attgccgctg tcgatcattg
     4981 cctggaactt gttagcaatc gccaggaagc cgactttcgc atccgggcta aacgctttct
     5041 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     5101 cgtaacgttg acgtttcatt ttgaacagca ccacagcaca gagcatcagc gccatccctg
     5161 ccagcatctg gttggcaata ccaaacagcg gccacaaggt gttaataccg cccagcggat
     5221 cgaccacgcc ctgatgcagg aagtagcccc acgccagcac gcacagcgcc gttgccagca
     5281 ggttagcagg cagtgaatcg gtccgtttca ggcccggaga caccacgccc agcagatcct
     5341 gcaacataaa gcgcgcagca cgcgtacccg catccaccgc cgtcagaata aacagtgctt
     5401 caaacagaat ggcgaagtga taccagaacg ccacatccat catgccgccc agcgcaccgt
     5461 gcagaatgta ggccatcccc accgccaggg tcggcgcacc gcccgcacga gaaatgatcg
     5521 actgttcacc cacttcgctg gcaatctggt ttagcgtatc cggggtaatc gcaaagcccc
     5581 agctactcac cacctgcgcg gcagaagcaa ccacatccgc cgtccctgcc ggagccagca
     5641 ccgccatcgg gctgttcatg gcgaagtaca cacccggatc gatgatacag gcggaaacca
     5701 gcgccataat cgccacgaag gattccatca acatcccacc gtagccgata aagcacgcct
     5761 gcccttcgtt cgccagcatc ttcggtgtag tgccggaaga gatcagtgca tggaagccag
     5821 acaccgcgcc acaggcgatg gtgataaaca ggaacgggaa caggttaccg gtccataccg
     5881 ggccagtgcc atcgacaaat ttagttagcg caggcatggt cagcgtcggg cgcataatca
     5941 aaatgcccac cgccagaccg acgatagtcc cgattttcag gaaggttgag aggtagtcac
     6001 gcggagccag cagcaaccac accggcagca ccgccgccac aaaaccgtaa cccaccagca
     6061 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccacgtcggg ctttctgcca
     6121 cccagccgcc agagataatg gcgaaaataa ggaacaccag accaatcacc gacacttcgc
     6181 caatacgccc cggacgcaaa tagcgcaggt agatccccat aaacagcgcc agcggaatgg
     6241 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     6301 ccagcacggc aaggataatg accatgatca taaagcaggc caccagcgca atcaccccgg
     6361 cggttggccc catctcttct ttgaccaact cacccagcga gcgaccatcg cggcgcgtag
     6421 aaacaaacag caccatgaaa tcctgcaccg caccggcgag aaccacccca gcgagcagcc
     6481 agatcatccc tggcaggtaa cccatttgcg ccgccagtac cggcccaacc aacggacctg
     6541 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt tttgtccgtc ggcacatagt
     6601 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     6661 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     6721 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     6781 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     6841 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtacaat
     6901 ttaacaacac ggcaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     6961 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     7021 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacaa
     7081 caatttccca gctctgattt tgccgaccaa gatgcagcgg ctggcagacg ccgcgtacct
     7141 ttccctcaga caccgggcga tgatgtgtgg cgttaagttc cgtgcccacc acacactgtc
     7201 cgtcgcgagt catcataaat ccggccatcg atcccagcgt ttccgccagc gccgccgacg
     7261 cgccgccatg tagtaaaccg aacggctgat gagtacgggt atcaaccggc atttcggctt
     7321 ccagcacatc atcgcccagg cgggtataca caattcccag atgcgccacc attgtgttat
     7381 cgctggtggc gttcagctcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     7441 cagcgtagag ccgccatcta ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     7501 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     7561 gccaagttta aactgctcgc caaagccgcg aatacgctgc tcttcggcgt catcgctcac
     7621 ccacagcgtg cgttgcatat cggtgtcggt ggagcctggc gaaaccacat tacagcgcac
     7681 gccgctaccc gccagttcca gcccaacgct caacgccagg cttttcagcg ccgctttcga
     7741 cgcgccataa gcactcatac caatgcgcgg cgtgtgtgcg gcgtcggacg caacagtaac
     7801 aatcgccccg ccccgctgac ggcgaaactg gttcatggtc tgctggaaca ggttaaacgc
     7861 accgccgaca ttaaccgcaa aagtctgctg ccagtcctcc ttactgagct gatcggtcgc
     7921 gcccatgcgc aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa
     7981 cagtcgctga catacctgcg ccacctgcgc agcgtcggca acatccatca cttcggtcgc
     8041 aaagggatat tgctcctgag tgaacgcttg atcaaaacct gtaactttcg ctcccgcctc
     8101 aacaaacgcc agcgccgtgg cgtagccgat acctttcccc gcgccagtta cccagacatt
     8161 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     8221 gtcgggtttt tcgccagcat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc
     8281 gccagcgcca tcatccgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     8341 ggttcatcgg actcgtccag caacggcagg atcacctcac gcagcgccgc tttgctggcg
     8401 gggataggtg ctggcagtaa ttcttcggtc atcaccactc ggccagaacg tccggccaca
     8461 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc tgccaccata
     8521 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     8581 tataccccgg taataatcag ctggttacgt ccactctctt tcagcatttg ctccagcggg
     8641 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     8701 aggctatcca ccaccttttg ctgttccggc gaacgggtca ggccaggccc ccacatatca
     8761 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg
     8821 atattgtgct gtttgcagta gtcgcgtagt gccgcaatat tcgcgatcac ctgctccatc
     8881 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     8941 gcggcacgtt gcggttcaaa ggcccagtcg actttattct gcggaatatc gtgagactcc
     9001 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
     9061 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
     9121 aagtgaatct acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
     9181 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcacagct
     9241 tttttcgccc atcagctcat cttccatgct caccagtgcg gcgtagatca ccgccggatg
     9301 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg
     9361 atctttctcg cgcccctgca cagtgatgta accctctgga tcaatagaga tcagatcgcc
     9421 ggaacagtaa aaaccgttgg catcaaaggc gctggcattg tgctgcgggc ttttgtaata
     9481 gccacggaag gtatacggac cgcgggtcat caggcgtcca acttcccctt gcggcagtgg
     9541 atttccttcg gcatcggcaa cccacacttc atcgtccgga cacattgggt aaccctgggt
     9601 atggataatt ttctccgcgc tatcatcaag tcgggtgtag ttcactagtc cttccgccat
     9661 gccgaatacc tgctgtaact ggcagccaat ctctgcggga atacgcgccg caagcgtggc
     9721 agaaagacgt gcaccaccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
     9781 gccttcagcc agtgcctgca accacaggct gactgcgggc ggcaccagcg cggtaacgtt
     9841 gacctgatgt ttttcaatca atgggaagca aagcgtggcg ctggggtcgg cagccagaac
     9901 gacagtgccg ccagcgagaa atacccccaa cgatcccggc gaactcatgg cgtagttatg
     9961 agccgccggg atcgcgcaca ggtagcgcgt ctgttgtgtg aactgacaaa tctcgacgct
    10021 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggt atcagtttcg gagtgccggt
    10081 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggagatggcg tggcagtaaa
    10141 atcctcagcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagcag
    10201 ctgcaccacg cgaatggaag aatgctctgc aacaaatgtg ttgaggaaat catccccgct
    10261 aaatagcgca tgttgacgat cggcaatcag taacgccggt tcaatctggc tggcataggc
    10321 gttcagttca ctacgctgat ggctgaacag cgccagcacc ggcgcaacgc ccagcttcag
    10381 cagcgcgaaa aaggtaatat acaattcagc gacgttaccc agttgtacca gcgcggtttc
    10441 accaggttta atgccctgac ggcgcaaact acaggcgagg ttatccgccg cctgattcag
    10501 ctcccggtaa ctcaactgcc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
    10561 gcgagtcaga atgtcggtca gtggcaaatc ctgccagtag cctttttccc gatagcgacg
    10621 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
    10681 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
    10741 ggacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
    10801 gcagcggatg gtcaccaccc attcaccgtt accttcgctg tcacaccaac ccacaatgcc
    10861 gccaaacagt tcgcggtcga acggctccag ttcagcaata acctgagtcg cggcctgatg
    10921 cgggaaaccg ctcagcgcgg gggtcggatg cagcagacag gccagagtca gtgcgttttc
    10981 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
    11041 caattgtgga gaggagggaa cgtgtaactc actactgcgt ttgcgcagta cctctttcat
    11101 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
    11161 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
    11221 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
    11281 gccatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    11341 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaacc gtgacaacac
    11401 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    11461 cgtggtttgt tccggaattg cctggcgttc caccacattc agcgactggc tgcgggtgaa
    11521 acggcgtgcg gaagcttgtt tttcctgacg ggagaacgac tgccaggatt cgggaatata
    11581 cagcgatgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    11641 gccctgcgct ttggcaccgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    11701 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    11761 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    11821 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    11881 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    11941 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    12001 cattaacttc attaataaca ggaagctgat ttgtgagact cgtcccgctc taccgcaacg
    12061 cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc gccgactggc
    12121 cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg cagcgtattg
    12181 tttccaccag cgtcaccctg actggctcac tgctggcgat tgatgctccg gtgatcgcca
    12241 gcggcgcgac cacgccgaat aaccgcgtcg cggatgacca gggcttttta cgccagtgga
    12301 gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgagccg agtgcagaaa
    12361 ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggt gattcagcgc
    12421 tggcgctgta cgatcagctt tccaccatcg ccccaacatt aatcatcaat tacgacgaca
    12481 aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag aaacaagcgg
    12541 cagagcggat tgcacagttt gataagcaac tggcggcggc gaaagagcaa atcaaattac
    12601 cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc aatctctgga
    12661 cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgttg gcgaaattgc
    12721 ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt cagcttggtg
    12781 gggaaaatct ggcttcaggg ttaaatggcg agtcactatt cctgttcgcc ggtgatcaga
    12841 aagatgccga tgcgatttat gctaatccgc tgctcgcgca cctgcctgca gtacaaaaca
    12901 agcaggttta tgcgctggga accgagacgt tccgtctgga ttactacagc gccatgcaag
    12961 tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt cacctgcggc
    13021 ggcgtctggc gaaaacgccg caactccacc agcaccagca ataacaacac gccgataatc
    13081 aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc gcccaaaccg
    13141 cccagcagcg ccgcgcctat ggcatcgccc gtcacgtttt gcgccgtcca caaaccgtta
    13201 atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtgtattg cagcaacgaa
    13261 ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat ccacatcggc
    13321 atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggaaagcag catcaataac
    13381 cctggtcgcg cgctatgtgc cagcttcccg ctggttaacg cgccaatagc cgcaccgagc
    13441 gggatcgccg catagagaaa accaatctgt gccgccgaca tctgccagtt gtcagccagc
    13501 gccggataca ggacccgcac cgcgctcgcc atcgtcaata aaccacccaa cagcgcaatc
    13561 cctcctacca gcgggctggc gagcagaaaa cgaaatcctg ccagtaatga tttcaacgga
    13621 tgctcacgcg gctgcggtgg tggtggtaac gccggaaggc ttaacaacgg tagcaaggta
    13681 ataaacgtgc ccgccgccgc cagcccgtag ttccaggcca caccgccagt cgccagcagt
    13741 aaaccgccaa tcatgggcga aatcaccgat cccagacgca cggtcaacat ggtgatcgcc
    13801 ccggcctgca ttaagttttc acgccctacc agtgctggtg tcgccgccag tagcgccgta
    13861 acgccaagtg atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac
    13921 ggctccggca gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc
    13981 gccagcaaaa tcactttttt gcgctcatag cgatccgcca gcacaccgcc gaccatcagg
    14041 ccaacaaaca tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgc
    14101 gtcattatct ggatctgcac cggtaccgcg acgccgagca aacccagaga cacaattgag
    14161 ataaaacgag cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta
    14221 agcagccagg attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt
    14281 cttaggctgc acatgctaac atatccaaat aagatcgata acgataatta atttcattat
    14341 catggaagtt cgtatgtctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt
    14401 gctgttatta ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc
    14461 cgcttccgta gtgctggagg ccctctccgg cacctgccag agcgccgact gcaccatcgt
    14521 gctcgacgcg cggctgccgc gcacccttgc cggtttactg gcaggcggcg cgcttgggct
    14581 tgccggggcg ttaatgcaaa ccctcacccg aaacccactt gccgaccccg gcttgcttgg
    14641 cgtaaacgcc ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc
    14701 cgcgcaggaa caactggcga tggccttcgc cggggcgctg gtggcctcgt tgattgttgc
    14761 ctttaccggc agccagggcg gcgggcagtt aagtccggtg cgtttaaccc tggcgggcgt
    14821 ggcgctggcg gcggtgctgg aagggctgac cagcggcatc gccctgctta atcctgacgt
    14881 ctacgatcaa ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tatatacctt
    14941 aaaagttgtg ctgatcccgg tgctgatcgc cggagcaacc gcgctattac tgagccgcgc
    15001 gctgaacagt ttaagcctcg gcagtgacac cgcgacagcg ctgggcagcc gcgtggcgcg
    15061 cacacagttg attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcggtagt
    15121 tggcccgatt gcctttattg gcctgatgat gccgcatatg gcgcgttggt tggttggagc
    15181 agatcatcgc tggtcgctgc ccgtcacgct acttgccacc cctgccctgc tgctgtttgc
    15241 cgatatcatc gggcgggtga ttgtgcccgg cgaactgcga gtttccgtgg tcagtgcgtt
    15301 tattggtgca ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gcgcatgatt
    15361 tacgtctctc gccgattaat tatcacctgt ttgctgctgg tttccgcctg tgtggttgca
    15421 ggtatctggg gattacgcag cggtgccatc acgctggaaa cctcgcaggt attcgccgcg
    15481 ctgatgggcg atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc
    15541 gtgctgatgg cgctgttgat tggcgcggcg ctgggcgtca gcggcgcgat ttttcagtcg
    15601 ctgatgcgta acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcatggagc
    15661 ggcgtactgg tggcgatggt gctgtttggt caggatctga cggctatcgc gctggcagca
    15721 atggcggggg gtattgtcac ctcgctgttg gtctggctgc tcgcctggcg taacggtatc
    15781 gacacctttc ggttgattat tatcggtatc ggtgttcgcg ccatgctggt ggcctttaat
    15841 acctggctgt tgctgaaagc gtctttagaa acggcgctaa cagcgggttt gtggaatgcc
    15901 ggatcgctca acggcctgac gtgggcaaaa acctcacctt ccgcgccaat cattatattg
    15961 atgctcattg ccgcctcctt gctggtgcgg cgaatgcgct tgctggaaat gggcgatgat
    16021 accgcatgtg cgctgggcgt cagcgtcgaa cgttcgcggc tgttaatgat gctggtcgcc
    16081 gtagtgctta ccgctgcggc aacagcgctt gccgggccga tttcctttat tgctttagtc
    16141 gcgccgcaca ttgcccgccg cattagtggc accgctcgct gggggctaac ccaggcggcg
    16201 ctgtgcgggg cgttgttact gctggtggcc gatctctgcg cccaactact gtttatgccg
    16261 tatcaacttc cggttggcgt cgttaccgtc agcctcggtg gtatttacct tatcgtcttg
    16321 ttaattcagg agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt
    16381 taaccctcgg atatggcaaa tataccgttg cggaaaatct gactgtagaa atacctgatg
    16441 gtcacttcac ggcaattatc gggccaaatg gctgcggtaa atccacgttg ctgcgtacct
    16501 taagccgcct gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac
    16561 attacgccag taaagaggtt gcacgccgga ttggcctgtt ggcgcaaaac gccaccacgc
    16621 cgggagatat caccgtgcag gagctggtgg cgcgtgggcg ttacccgcat caaccgctgt
    16681 ttacccgctg gcgcaaagaa gatgaagacg cggtaacgaa agcgatgcag gccacgggaa
    16741 tcactcatct ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgagcgt
    16801 ggatagcaat ggtgctggcc caggaaacgg caattatgct gctcgatgaa cccacgacct
    16861 ggctggatat cagtcatcag attgatttgc tggaactgtt aagcgaactg aaccgcgaga
    16921 aaggctatac cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc
    16981 atttgattgc attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca
    17041 ctgctgaact gatcgagcgc atttatggtc tgcgctgcat gattattgac gatccggttg
    17101 ccggaacgcc gctcgtggtg ccgctcggac gaacgacacc ttcaaccgcg aagatttaaa
    17161 ctaagtggtc tgccattatg gcatcctgtt ttctggatgc catcgcatgg cgcaatagca
    17221 cgccaccaca agccactata ccgccgatca acgcagaaag gatcacaata atcgccttac
    17281 ccggaccgtc tttttttacg ggtaatgacg gacgcaactg atatttaaac ggtgtaaaat
    17341 tcacatcgtt gacatttgtt tttgttaatt gctcgactag atactgacga ttacgtaatt
    17401 caccgttcaa ttccgcaacg tcagtgactg ctttttcaat ttccagtttg cgttcaatac
    17461 cgtctgcgcc gagagaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt
    17521 atacgggctt tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa
    17581 tgtttgcatc aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa
    17641 actgggtttt gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg
    17701 cagagatata atcgatatac ccggccaaca ctttctgtgc ctcttcactg gtgggtgcgg
    17761 tgaaacttag cgtccaggaa gtatacaatg cagactcatc ttttttctta ctggcattgt
    17821 catcaaccgc tttcattttt tcgctcaatg cgacaattgc gcgatgcaaa tccagttcgt
    17881 cgattttcgc ctcttttaat tggtccatca cataaggtgt tgagcgcagg tactcttcca
    17941 gcaagctaac cgactgaaac ttcttgataa acaggttaaa tgcttctgta cgatcaattt
    18001 tgatatccag atccagcaca cgaagcttag tgaatgtttt ctcaagcgcc tgccactgaa
    18061 caggttctgg aggcgtgaca accgccgcgc tggtccattt ttgcggcagg atgaaagaga
    18121 tcagtaaacc tgcgcaggca aacgcaaaaa cgaccgccat gaccgttttt ttggcccgcc
    18181 ataaaacttc aattagatta agtaaatcaa cttcattatt actgggcgac gccagaggat
    18241 actcgggaaa atgagcttca cttccctgtt taatattcag tgatgacatg gggtataacc
    18301 tgaaaatgag tccaataaga gtaaatctcc acggccccca ctctaccagt tggtgagatt
    18361 tatccgaagc tgaagtttgt aaattactcc cactgaatgc aacattcact cta
//
LOCUS       DAZGHF010000093        18110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGHF010000093 DAZGHF010000000
VERSION     DAZGHF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.093"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(105..1133)
                     /gene="epmB"
                     /locus_tag="ACHK3A_003349"
     CDS             complement(105..1133)
                     /gene="epmB"
                     /locus_tag="ACHK3A_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO8741395.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1175..1741
                     /gene="efp"
                     /locus_tag="ACHK3A_003350"
     CDS             1175..1741
                     /gene="efp"
                     /locus_tag="ACHK3A_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO8741396.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1793..1918
                     /gene="ecnA"
                     /locus_tag="ACHK3A_003351"
     CDS             1793..1918
                     /gene="ecnA"
                     /locus_tag="ACHK3A_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO8741397.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2029..2175
                     /gene="ecnB"
                     /locus_tag="ACHK3A_003352"
     CDS             2029..2175
                     /gene="ecnB"
                     /locus_tag="ACHK3A_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO8741398.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2350..2667
                     /gene="sugE"
                     /locus_tag="ACHK3A_003353"
     CDS             2350..2667
                     /gene="sugE"
                     /locus_tag="ACHK3A_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO8741399.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2668..3201)
                     /gene="blc"
                     /locus_tag="ACHK3A_003354"
     CDS             complement(2668..3201)
                     /gene="blc"
                     /locus_tag="ACHK3A_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO8741400.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3290..4423)
                     /locus_tag="ACHK3A_003355"
     CDS             complement(3290..4423)
                     /locus_tag="ACHK3A_003355"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO8741401.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            complement(4486..4845)
                     /gene="frdD"
                     /locus_tag="ACHK3A_003356"
     CDS             complement(4486..4845)
                     /gene="frdD"
                     /locus_tag="ACHK3A_003356"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO8741402.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4856..5251)
                     /gene="frdC"
                     /locus_tag="ACHK3A_003357"
     CDS             complement(4856..5251)
                     /gene="frdC"
                     /locus_tag="ACHK3A_003357"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO8741403.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5262..5996)
                     /gene="frdB"
                     /locus_tag="ACHK3A_003358"
     CDS             complement(5262..5996)
                     /gene="frdB"
                     /locus_tag="ACHK3A_003358"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO8741404.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(5989..7797)
                     /gene="frdA"
                     /locus_tag="ACHK3A_003359"
     CDS             complement(5989..7797)
                     /gene="frdA"
                     /locus_tag="ACHK3A_003359"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO8741405.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8122..9099
                     /gene="epmA"
                     /locus_tag="ACHK3A_003360"
     CDS             8122..9099
                     /gene="epmA"
                     /locus_tag="ACHK3A_003360"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO8741406.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9318..10820
                     /gene="yjeM"
                     /locus_tag="ACHK3A_003361"
     CDS             9318..10820
                     /gene="yjeM"
                     /locus_tag="ACHK3A_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO8741407.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            10883..11197
                     /gene="yjeN"
                     /locus_tag="ACHK3A_003362"
     CDS             10883..11197
                     /gene="yjeN"
                     /locus_tag="ACHK3A_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO8741408.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            11194..11508
                     /locus_tag="ACHK3A_003363"
     CDS             11194..11508
                     /locus_tag="ACHK3A_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO8741409.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(11539..14862)
                     /gene="mscM"
                     /locus_tag="ACHK3A_003364"
     CDS             complement(11539..14862)
                     /gene="mscM"
                     /locus_tag="ACHK3A_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO8741410.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLLGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14884..15852)
                     /gene="asd"
                     /locus_tag="ACHK3A_003365"
     CDS             complement(14884..15852)
                     /gene="asd"
                     /locus_tag="ACHK3A_003365"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO8741411.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDANPLVDDNKDQV"
     gene            complement(15949..17001)
                     /gene="rsgA"
                     /locus_tag="ACHK3A_003366"
     CDS             complement(15949..17001)
                     /gene="rsgA"
                     /locus_tag="ACHK3A_003366"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO8741412.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            17096..17641
                     /gene="orn"
                     /locus_tag="ACHK3A_003367"
     CDS             17096..17641
                     /gene="orn"
                     /locus_tag="ACHK3A_003367"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO8741413.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17852..17927
                     /locus_tag="ACHK3A_003368"
     tRNA            17852..17927
                     /locus_tag="ACHK3A_003368"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17885..17887,aa:Gly,seq:gcc)
BASE COUNT         4184 a         4632 c         4775 g         4519 t
ORIGIN      
        1 aggttaatta ttggtgttgg ttatataaaa agtaataaca gcaatgtata tgaataatta
       61 aaggttatgc aacgtgcaaa gattaaactt ccctgtttaa tcagttactg ctggcgtagc
      121 tggagatcca gcggcgtttt gctgggttcg ccgccaattt ctcgcgccag tttaggcacc
      181 agatatcccg acaccagtgt cagcaactca cgcataatct gccgtgcttc gtcatcactc
      241 accataaaat gcgccgcgcc ctgtacttta tcgagcacat gcaggtaata cggcatcacg
      301 cccgcatcga acaacgcatt actcaggttt gccagcgttt gcgcgttatc gttcacacca
      361 cgtaacagaa cgctctggtt gagcaaagta acgcctaccc ggcgcaactt agccatcgcc
      421 tgacggaatg tttcatctac ctcattggca tggttaatgt gattcaccag caagatttgc
      481 agcgtagaat gagaaaagcg ttcaaccagc gcatctgtga tgcgtgccgg gatcacaatc
      541 ggcagacggc tgtgaatccg cagacgtttg atatgcggga tggcttccag ttgtgtgagc
      601 aaccagtcca gctcgtgatc tttcgccatc agcggatcgc cgccggagaa aatcatctcg
      661 tccagttccg gatgcgcagc aacatactca agtgcagtct gccagttacg cttgttgccc
      721 tgattttcgg cataggggaa gtggcgacgg aagcaatagc ggcaatttac cgcgcagccg
      781 cctttgacca gcaaaagcgc ccggttgtgg tatttatgca acaaacctgg cactacgctg
      841 tgctgttctt ccagcgggtc ggtggagaat ccgggcgcga caacaaactc atcttgcgag
      901 gtaagtacct gacgcaaaag cggatcgttc ggattgcctt tctccatgcg atcgataaat
      961 gagcggggca cacgcagggc aaacagcttt ttggcgctgc gcccggccaa cagtttttcg
     1021 tccgcgtcta tattcaaaag acgcagaagt tcatcaggat cggtcacaac atcggcaagt
     1081 tgcgttaacc aatcttctct ggatggggta tttagggtta caatatgcgc cattttgtgg
     1141 cttagctacc aattaacaaa tttcagaggg ccttatggca acgtactata gcaacgattt
     1201 tcgtgctggt cttaaaatca tgttagacgg cgaaccttac gcggttgaag cgagtgaatt
     1261 cgtaaaaccg ggtaaaggcc aggcatttgc tcgcgttaaa ctgcgtcgtc tgctgaccgg
     1321 tactcgcgta gaaaaaacct tcaaatctac tgattccgct gaaggtgctg atgttgtcga
     1381 tatgaacctg acttacctgt acaacgacgg tgagttctgg cacttcatga acaacgaaac
     1441 tttcgagcag ctgtctgctg atgcaaaagc aattggcgac aacgccaaat ggttgctgga
     1501 tcaggcagag tgtatcgtaa ctctgtggaa tggtcagccg atctccgtta ctccgccgaa
     1561 cttcgttgag ctggaaatcg ttgataccga tccgggcctg aaaggtgata ccgcaggtac
     1621 tggcggcaaa ccagctaccc tgtctactgg cgctgtggtt aaagttccgc tgttcgtaca
     1681 gatcggcgaa gtcatcaaag tggatacccg ctctggtgaa tacgtctctc gcgtgaagta
     1741 atgcggttgt ggtgcggcct gcaggctgca ccatcactta ttcaggtcag agatgatgaa
     1801 acgccttatc gttcttgttt tgcttgccag tgcgctgctc acgggctgta acaccgctcg
     1861 cggttttggt gaagacatta aacatctcgg caactccatc tcccgcgctg ccagctaatt
     1921 tttcttctcc tccgaaaaat catcagattt ccatcatttt tggcgatgtt gtctattatt
     1981 aatttgctat aggcaaacat aaataacatt acctaaaagg aagacgttat ggtgaagaag
     2041 acaattgcag cgatcttttc tgttctggtg ctttcaacag tattaactgc ttgcaacacc
     2101 acgcgtggcg ttggtgaaga catttctgat ggcggtaacg cgatttctgg cgcagcaacg
     2161 aaagcgcagc agtaagcaat aacggtacga cagctgtgtc gtgccgtttg tttttttgcg
     2221 atagtcacaa aggtaatagt tgaaattccc ctgccacctg gcaaaatatc cgttcaacca
     2281 tcagctttgc aggacgacct gcaaacgcct cttttcaccg gggacggccc caattctccg
     2341 gagcctgata tgtcctggat tatcttagtt attgctggcc tgctggaagt ggtatgggcc
     2401 gttggcctga aatataccca cggctttagt cgtttgacgc caagtgttat tactgtgacg
     2461 gcgatgattg tcagtatggc gctacttgcc tgggcgatga aatcgttacc agtagggacg
     2521 gcttatgccg tgtggacggg tattggcgca gtcggcgcgg ccatcaccgg cattgtgctg
     2581 ctcggcgagt ccgctaaccc gatgcgcctg gcgagtctgg cgttaatcgt attggggatt
     2641 attggtctga aactcagcac tcactaacta actaccgggc tgctgtaccc aaataaactt
     2701 actgacatca aacccttccc gggtcgcgac tgccagcatc tcctgtttca cttcgtcaga
     2761 aatggttggc gtgcgggaga gtatccacag gtagtcgcgg tccggaccgc aaaccagcgc
     2821 atggcggtat tcccgatcga gtgcaataac gttataaccg ccatagaaag gaccaaagaa
     2881 tgacactttc agcgcagcgc gagttggtgc gccggtaaag tacgctttcc cttcactctg
     2941 ctgccacatt tctctgtcag ggttatagcc tttattaatg acattcaggc cgccgtcatc
     3001 acgcaggctg tatgttgcgg tgactttttc cagtccacgt tcaaaacggt gatcaaaacg
     3061 ggcaatctca taccaggtac cgagatagcg tttagcatcg aaattattca ctacggtcac
     3121 gccacggggc ggcgtaggag aactgcaggc aacgaccaga aatgcagctg tcgctgcggc
     3181 aacgagaggg agcaggcgca taaatgtttc cttactggtt tttttctaag tgtagatgac
     3241 agcaaggaaa agcggagaaa aggtccaaaa attcggaccc gatgaaattt tactgtagag
     3301 cgttgagaat ctgacaggcg gcggcgactc tcgctggatt gggatagttt ttgttagcca
     3361 gcatcacgat acccagctct ttttctggaa taaacgcgac atagctacca aatccgccgg
     3421 tcgcccccgt tttatgtacc catgatgcgc gtactgcagg agttgggggc gtaatcgctt
     3481 ttacggggcg tgctgccagt gcaattttat tgtcactgcc gttaatgatg atgtcaggat
     3541 tgaccggcca gtccagcatt tcccagccca ggccctgata catatcgccg gtttgccagt
     3601 agcgagattg tgccagttgt atcccttgtt gaagcgtttt ctcagtgata tcaaggggtt
     3661 ttaaattgct ttgcacccag cgggccatat cttcaatggt cgacttcaca ccataagcct
     3721 cagcatctaa cgcccctggc gaaacatgca ctgccttacc ttcgcgatat ccccaggcgt
     3781 aattcttttc ttctgcggac ggcacattaa tccacgtatg agtgagtttg agtggctgga
     3841 aaacacgagt ttgcatcgcc tgttcaaagc tcaaaccgga tggtttcacc gccagtgcac
     3901 cgaacaaccc aatactggcg ttggcataca gacgttgtgt tcctggagcc catgcaggtt
     3961 gccagttttg atagaagcgc agcaagtcgc ttgaggattt aacgtcatcc ggcacctgca
     4021 atggcaggcc gccagcggtg taggtcgcga gatgtaatag tgtgatccca ttccattgtt
     4081 tagcggtaag ttcaggccag tattttgtcg cgggatcgct taacttgatt tcccctcgag
     4141 caatagcgtc gccaccaagc acgcccgtaa atgttttgct gaccgaacct aactcaaaca
     4201 acgtttgctg tgtgacgggc tgctttttgg cgatgtccgc atagccccag gtaaagtaat
     4261 aaggtttacc ctgataaatt accgccaccg ctataccggg gatcttttgt tgctctataa
     4321 gcggggtaat tgtgcgatgc acaatatcgt tgatttgttg aggggcagca aatgtggagc
     4381 aagaggcggt aattaataag gcgcagagcg tcattttgaa catagggtct ggtttccata
     4441 caaaacggcc cgccataggc gggccggatt tacattggcg atgcgttaga ttgtaacgac
     4501 accaatcagc gtgacaactg tcaggatagc agccagaccg tagaaaaccc atttgcccgc
     4561 aggtacgtgg attttcagat cgtgcatcgc gtggtgcata cggtgtaaac cacaccacag
     4621 cggcagaacg atcatcagga acaggaatac gcgaccaatg aagctctgcg cgaacgccag
     4681 aacgcgctcg tagctcagtg cgtcacccgg aaacagcccc agtggcagca gaataccaac
     4741 cagcaggatc atcaccggcg caataattgc gctccacata ccaccggccc cgaagaggcc
     4801 ccagaatacc ggttcgtcag aacgctttgg atttggatta atcatctcag gctccttacc
     4861 agtacagggc aacaaacagg attacgatgg tggcaaccac agttaccgcc cagaggcttt
     4921 tgataattgg ctctggtccc attttttcgt cttttacaat gatattggcc gcttttggtg
     4981 ccagctcaaa ccaggttttg gtgtgcagca gggctgccgc cagagtgatc aggttaatga
     5041 tcacgataac cgggttttgt aaaaagtcga cgaatcccgc ccaggcttcc gggccatttt
     5101 tcagggcaaa cagcccgaaa atcagttcaa tgctgaacca cacagccgga accgccgtgc
     5161 cttcgcgcag catgtagaag cgataaaacg gcaatttttt ccaccaggtg gacgtcattg
     5221 gccgtacata cggtttacgt ttagtcgtca tgttgcactc cttagcgtgg tttcagggtc
     5281 gcgataagaa agtctttcga actttctact ttgccctgct gaatggccgc agccggatcg
     5341 acgtgtttcg ggcagacttc ggagcagtag cccacgaaag tacagctcca tacgccgttc
     5401 tggctgttca actgcgccat acgctccttc ttaccgtggt cgcggctatc ttcgttataa
     5461 cgatgcgcca gcgtaatggc agccggaccg atgaactctg ggttcaggcc aaactgcggg
     5521 cacgcggcgt agcacagacc acagttgatg caaccggaga actggtgata cttggccatc
     5581 tgcgccgggg tctggatgtt agtaccctga tccgcggtgc gggagttgcc gatgatgtac
     5641 ggtttgatcg cttccagact ttcgatgaag tgggtcatat cgaccaccag atcgcgttca
     5701 atcgggaagt tagctaacgc ttcaaccttc ataccgtcgg tgtaatcacg caggaaggtt
     5761 ttacatgcca gttttggcac gttgttaacc atcatgccgc aggaaccaca aatcgccata
     5821 cggcaggacc agcggtagct caggtccggt gccaggttgt ctttgatgta gcccagcgca
     5881 tccagtaatg aggtagttgc gtcataaggc acttcataga atgcgctatg cggtgcggta
     5941 tcgacttccg ggttatagcg caccacctca attttcaggt ttttcatctc agccattcgc
     6001 cttctccttc ttattggctg cttccgcctt atcggctgca tccgcttcgc caccgtaaac
     6061 gcgtttagct ggcggcagcg tagtaatctt cacgtcgctg tactccaggc gagtcgtgcc
     6121 atcagcatcg cggaaggcga gggtgtgttt gaggaagttg acgtcgtcac gctcggtgca
     6181 accttcgtcc agacgctggt gtgcgccgcg ggactcttta cgtgccattg cggagtgcgc
     6241 catacattca gcaacgttca gaccgtggcc cagttcaatg gtgtagagca ggtcggtgtt
     6301 gaacacgctg gaagtgtcgg tgatgcgcac gcgtttgaag cgttcctgca gctctgccag
     6361 cttgtcgatg gttttctgca tcagttccgg cgtacggtag ataccgcagc cttcttccat
     6421 agccaggccc atttcgtcgc ggatcttcgc ccagttttcg ccgccatcct ggttaaccag
     6481 atctttcaaa cgttgttcaa cgccagctgc ctgcgcttca attaccgctt cgttgccatt
     6541 accggcagtt gctgcacgct ctgtcgcttg ttcaccggcc agacggccga agaccaccag
     6601 ttccgccagg gagttagaac ccagacggtt tgcaccgtgc agaccaacag aggaacattc
     6661 acccacggcg aacagacctt taatgcgggt ttcacagttc tgatcggttt cgataccgcc
     6721 catggtgtag tgtgcggtcg gacgtaccgg aatcggttct ttaaccggat cgacgccaac
     6781 gtacgctttc gccagttcgc agatgaacgg cagacgttca tgcagttttt tctcgccgag
     6841 gtgacgcaag tcgagataaa ccacatcgcc acgcggcgtg gagatagtgt tgcctttacg
     6901 ccattcgtgc cagaaggcct gagaaacttt gtcgcgtgga cccagttcca tatatttgtt
     6961 tttcggctcg cccagcggag tttccgggcc catgccgtaa tcttgcagat aacggtagcc
     7021 atttttgttg accagaatac cgccttcacc gcggcagcct tcggtcatca ggataccgga
     7081 acctggcaga ccggttgggt gatactgaac gaattccatg tcacgcagcg gaacgccgtg
     7141 gcttagcgcc atacccatac cgtcaccggt aacgatgccg ccattggtat tgtaacgata
     7201 aacgcgaccc gcaccgccgg tagccataac gaccgcgtta gcacggatct gcaccagcgt
     7261 gccttccatc atgttcattg ctaccaggcc gcgaacatga ccatcatcaa ccagaatatc
     7321 cagtacgaaa tgttcgtcaa aacgctggat ctgcgggaat tgcagagagg tctggaacag
     7381 cgtgtgcagc atatggaagc cggtcttatc ggcggcgaac caggtgcgct cgattttcat
     7441 gccgccgaag cgacgtacgt tgacgctacc atccgggcga cggctccatg ggcatcccca
     7501 cagttccagt tgggtcattt cggtggggca gtggtggacg aaataatcca cgacatcctg
     7561 ctcacacaac cagtcgccac ccgctactgt atcgtgaaag tgatattcga agctgtcatg
     7621 atcctgcgcg acagcggcgg agcccccttc tgcagcaacg gtatggctac gcatcgggta
     7681 tacttttgag attagtgcga tttttgcatt tggatttgcc tgcgcggcag caattgcagc
     7741 acgtaatccc gcgccaccgg cgcctacaat ggcaagatcg gcttgaaagg tttgcacgac
     7801 attcctccag attgttttta tcccacagcc ccgtacttca gggtaagtgc ctgaaagtta
     7861 cggtctgcga acgctattcc actgctcctt tataggtaca acagtatagt ctgatggata
     7921 agtctgaaat ttgacgagat cgattttttt agtgcgcaag agggctaaat tatcactgaa
     7981 gatgattaat ttaattacta aaccatcaga tcgtgctttt tttagtcact gcccaccctt
     8041 gtttgattcc tgcgtaaaat ttgtctcgcc gtcgcgttgc gagtagactt cgtgcccttg
     8101 tcaaaaactg gagatttaac tatgagcgaa acggcatcct ggcagccgag cgcatccatt
     8161 cctaacttat taaaacgcgc ggcgattatg gcggagatcc gtcgtttctt tgccgatcgt
     8221 ggagtgctgg aggtggagac gccttgtatg agccaggcga cggtaaccga tattcatttg
     8281 gtcccgtttg agacacgttt cgtaggcccc gggcattcgc aggggatgaa tctctggtta
     8341 atgaccagcc cggaatacca tatgaaacgc ctgctggttg ctggttgtgg gccggtattc
     8401 cagctgtgcc gcagcttccg taatgaagag atggggcgtt atcacaaccc tgagttcact
     8461 atgcttgagt ggtatcgacc gcactatgat atgtaccggt tgatgaacga ggtggacgat
     8521 ctcttgcaac aggtgctgga ctgtccggca gcagaaagcc tttcttatca acaagctttc
     8581 ttgcgttatc tggaaattga cccactctct gccgacaaaa cgcaactgcg ggaagtggca
     8641 gcgaaactgg atttgagcaa cgttgccgat actgaagaag accgcgacac gcttctacaa
     8701 ttgctgttta cctttggcgt agagccaaat attggcaaag aaaaaccgac ctttgtgtac
     8761 cactttccag ccagccaggc atcactggcg caaatcagta ccgaagatca tcgggtcgct
     8821 gaacgctttg aggtttatta taaaggtatt gagctggcga atggtttcca tgaattgacg
     8881 gatgcccgtg agcagcaaca acgctttgaa caagataacc gtaagcgcgc ggcgcgcggt
     8941 ttgccgcagc accccattga ccagaatctg attgaagcct tgaaagtcgg tatgcctgac
     9001 tgttccggcg tggcattagg cgttgatcgt ctggtgatgt tggcgctggg cgcggagaca
     9061 ctggctgaag tcatcgcctt tagcgttgac cgggcataat tctgaaaaat tactgaatta
     9121 accgcgaaga atggcgtgat aagcgccatt ctgtagcata tgttttcatt tttccgctaa
     9181 aggccacaat ttaagactgg atatttgata tcatccaggt atcaattctg tattggtttt
     9241 tacgctgccc gctcaatgtt gggcagtgca gttcagtggg cgcgttttat acacgcgctg
     9301 aaatgaagga tgttttcatg cctcacacga taaaaaagat gagtctgata ggactcatat
     9361 tgatgatctt tacttccgta tttggatttg ccaatagccc atcggcttat tacttaatgg
     9421 gttatagtgc gattcccttt tatatatttt ctgcattgtt attctttatt ccattcgcct
     9481 taatgatggc tgaaatggga gctgcttatc gcaaagaaga aggcggtatc tattcctgga
     9541 tgaataatag tgtcggacca cgttttgcct tcattggtac gtttatgtgg ttttcctctt
     9601 atatcatctg gatggtgagt acctccgcga aagtttgggt accgttctca acattcctct
     9661 atggtagcga catgacccag cactggcgta ttgccggact ggagcctacg caggtggttg
     9721 gtctgctggc agtggcatgg atgattctgg tcaccgtcgt tgcttcaaaa gggattaata
     9781 aaattgcccg cattactgcg gtgggcggta ttgcagtaat gtgtctgaat ttagtattgc
     9841 tgttagtaag cattactatt ttgttattaa atggtgggca tttcgcgcag gatattaatt
     9901 tccttgcatc accgaaccca ggttatcagt ccggtctggc aatgctatcg tttgtggtat
     9961 ttgctatttt tgcctatggt ggaattgaag cggttggtgg tctggtcgat aaaacggaaa
    10021 atccagaaaa gaactttgcc aaaggtattg tttttgccgc tattgttatt tcaatcggtt
    10081 attcgctggc aatattttta tggggcgtca gcacaaactg gcagcaggta ttaagtaatg
    10141 gttccgttaa cctcggcaat attacctatg tgctgatgaa gagcctcggg gtgacgctgg
    10201 gtaacgcact gcatttgtca cctgaagcgt cattgtcgct gggcgtatgg tttgcgcgta
    10261 ttaccggact ttcgatgttc ctcgcttata ccggtgcgtt ctttacgctt tgctattcac
    10321 cgctgaaagc catcatccag gggacgccga aagcgttgtg gccggaaccg atgacgcgcc
    10381 tgaatgcgat ggggatgccg tctatcgcca tgtggatgca gtgcgggttg gttactgtct
    10441 tcatcctgct ggtttcgttt ggtggcggta ccgcatcggc gttctttaac aagctgacgc
    10501 tgatggcgaa cgtgtctatg acgcttcctt acctgttcct cgcgctggct ttcccattct
    10561 ttaaagcacg tcaggatctc gacagaccgt ttgtgatttt caaaacgcat atgtcggcaa
    10621 tgattgcaac agtggttgtc gtactggtgg tgacatttgc gaacgtcttc accatcattc
    10681 aacctgtggt tgaagctgga gactgggaca gcacattgtg gatgattggc ggccctgtct
    10741 tcttctcgct gttagcgatg gcgatttacc agaactattg cagtcgcatg gcgaacaaac
    10801 ctgagttagc tctcgactga tcatatgaaa tcctgcaact catcgtctct ccggcgatga
    10861 gttgtctgga aaggaagacg ttatggacga cgctagtcgg gatccagtga taaccgaaga
    10921 tgaaatcagg gagctgcaat tttctgccgg ggatgtggcg gaaatagagc aaacggtcct
    10981 ctcttttgtc gatacccgcc acacccgcaa agtagcgatg gtggtgggta acactattaa
    11041 taccctcaaa gagcgcgatg gtccgcggtg gggaaacctg ccggatatct attgcgctta
    11101 tcttattaga tgtctggttt ttcgcggaga attagtggga tacggcgatt tgtttcgtat
    11161 gcgctatagc gaaattaagc gaccgattat ctcatgagtg cgcgcatgtt tatcttgtgc
    11221 tgcatctggt ttattgtggc gttcctctgg ataaccatca cttccgcgct ggacaaagag
    11281 tggatgattg atggacgcgg gattaataac gtctgcgatg tgctgatgta tctcgaagat
    11341 gacgataccc gtgatgtcgg tgtgataatg acgttaccgc tctttttccc ttttctctgg
    11401 ttcgctttgt ggcgaaaaaa acgcggctgg tttatgtacg ccaccgcgct ggctattttc
    11461 ggctattggc tgtggcagtt ttttctgcgc tatcagtttt gcttataagc cggatgggtt
    11521 catccggcca caaagacctt acaaacttcc cgcctgacga cctttgcccg cagaagttaa
    11581 tgttctcccc gtttgcttac cattcaggct ttccaaacgc atctggaagg gcgggaatgg
    11641 catatcaata ccgtgggcat ggaatcctgc cagaattagc tggtgaatct catggcgtag
    11701 tggcatacgg tgacccatct cagcggcgta aatacgcagc tcgaaaattt gaatcccctg
    11761 ttgcagatcc accaggaaga cttccggtgc tgggttatcg attaccagcg agcagcgacg
    11821 cgctgcggtg agcaggattt ccgtcacttc ttcactgttg gcatcggcag gggccggaat
    11881 cgtcaacacc acgcgcgtga ccgagtcaga gagcgaccag ttgataaact gctcggtaat
    11941 aaacgccttg ttcggcacga taatttcttt gcggtcccag tcgctgatgg tggtggcgcg
    12001 ggtgttaatt ttcgtcacac taccggtaag atcgcggatc gttaccgtat cgccaatgcg
    12061 aatcggtttt tcgaacagga taatcaggcc agagataaag ttggcgaaaa tttcctgcaa
    12121 accaaaaccg agaccaacac cgagcgcggc gaccagccac tgcaatttcg accactcaat
    12181 accaatcatc gagaagccga ccagcccgcc aatcagcatc agcagatatt tggtgatggt
    12241 ggtgatggcg tagcccgtac ccggcgttaa atccaggtgc tgcagaatcg ccagttccag
    12301 cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata aacaccagaa tggcaatcag
    12361 caccgcaccg agggtaatcg gctccaggct ttctacgccc tgtaccgtgg aagtgacatc
    12421 ccacagcgaa atattttcga ggaagccgaa agcggaatgg atttctgacc acagcacaat
    12481 gaccgaaagc agggcgatca acatcaaaat tgagcgcacc agccgcaagg attgcgcact
    12541 gatggcatcg agatcgactt cgctttcatc gacttcaatt gctccttccg ggctactgtg
    12601 atgatgcgct tcctcttcgc cacgcgcacg ctgcgctaac atctctgccc ggcgatgctt
    12661 cgcccggtca aacgccagcc tgcggcgctg gatcagcatc cagcggcgga taacgtgata
    12721 aaccaccagt agcaggaacc agatggcaac cgaggtttca agcctcgcta acagcgcctg
    12781 cgccgttgcc agataaccca ccgccgacgc cagaatggca accagcggtg cgccaatcat
    12841 catgttccac agcatatggt tggtaatgtt gtcgccgctg ccttctttgt tgagatacag
    12901 cgggatcccg gcctttttca gactgagagt gaccaccgcc agcgcaccgc aaatgaggat
    12961 aaagcaaagc cgtcccagcg aaccggagaa ttcacggtcg tcgaggttat cgaacatcat
    13021 cagcgccata atcagcggca caataagccc gatgctcatc aggtagtagc gcatcccacg
    13081 ggaaacccgt tcgcgcggcc agccaaaatg agcgataaac aagccgttcg ggcgggcaaa
    13141 ggtggcgcaa atcatcacta cccacagcag cggcacggtg gctgttacgc catcaccaat
    13201 cgcgaccgcc agcggataag gccacgcctc gcgcaagccg taacccagcg tcatccacag
    13261 cacaggcagc ggtgacgcga cgagaatcga ccagaaaaga gtgcgtaacg tcagccagaa
    13321 gtgatcctga gtcactttgc cgactttcgc cgccgaacgt tcaagaaaac gggtgaaata
    13381 gcggcgcgag taaatactgc aaccgaccag aatcaacgcg ccaagcagcg gcaaaatcgt
    13441 ctctttactg gtcagcatca ttacactggc tttgcccaac tgactgaagg tgtccagcga
    13501 aatgagacga cgcagatcct gggcgatttc cagcggccag gcgatggtca tcgggcgcac
    13561 gtcagaggtc cagaacagat agcggtgcgt cgcttcattc acctctttca gcgcatcctc
    13621 cagttgcccg ttggagactt tcagcttggt cagttccagt agtagcgtgt ctccaccctg
    13681 caacaatgag ttcagcaact cacgctgagt gcgtagctgt gcttccagaa tacggttttg
    13741 ctcggcggtc agcggctgac cgtcggcctg atggatttgc cgtagcagcg gctgtttatt
    13801 tagcagatcc tcataacgta accgttgcac acgcaactgc gccatttcgg tatcaagctg
    13861 ttgtggtttt ggcatttccg gtagccgtgc cacctgcgcc cgcaacgctt cgccgagcag
    13921 gttggacgag cccagccatt gcgactgttc acgcagcgta ttcaacgcct gccggacctg
    13981 taacgtctgg ctggcagcct gacgctgttg cgaggcaacg agatccatcc gctgcgcctg
    14041 ttgattcagc gctgccgata gttcgcggtt aattttgaat tgcgcgacga tatctttcgg
    14101 caaatcggca ctgttttctg ccagtaattc ggtactttcc agcgcccgct ccgcctcaag
    14161 ctgacgttgg ctgttcaact gattacgtaa ggcctgcaaa tacgcatcca gttgctggct
    14221 ctctttttcc gccagctctg agcgtaagcg cgctaattcc tgacggttat tggcagacag
    14281 ctgcgccagt tccagttcat cgacgagcgc tttcaggcgt gcggagtcag actgcaacgc
    14341 gaaattttgt gcctgattga gcggggaatt gccggtaagc gtccccaggc ggcgctcgat
    14401 ttcatttaac tgacggcggg catcggtttg ctgttgcggc agttgattca gcgaatcggc
    14461 aatctcgcgg gcgcgttcct gctcttgctg ggcctgacgg cttttatcca gcaactggct
    14521 gctgacctgg agaatttcct gattcagcgc gtcggtagac attcccggtg acacgctgcg
    14581 cggttcgtca cgcatgttgt ttaattgtgc gcgcagagta gcggaaagtt tcgggtaatt
    14641 atcaatgact tcttgatatt gcttgatgcg ctcaagggaa ccttttcgtt cctcaagcgc
    14701 atttaaggcg gactggagcg cctctacgac ttccggctgc gcgggtttcg ccgcttttgc
    14761 ctgctccagt tcttgtgaga tttgtttgct atcgggggcc gtcgcggcgt acgcccccca
    14821 actgaggcac caggccatca gaaaagtgat aatcaggcgc acgtcagcgt ttcctttgat
    14881 ggattagacc tggtctttgt tgtcgtcaac caatgggttg gcgtcgtgtt ctgcttcgat
    14941 ctcttcagca ggaagcgggg ccggttcagc gtctggtgta acaaaggttt cggtagatac
    15001 tgccagcggc tggccaattt tcgtgacaga caggctttcc agttgctcaa ccagattcac
    15061 tttacccggt gcaaacaggt tgataacggt ggaaccgagt ttaaagcgac ccatttcctg
    15121 gcctttcagc agtgccacag aaccgtcgtt ttccccggca ggccaggtcc agcgcttgat
    15181 gataccttcg cgcggcggcg taatggtgcc cgcccagacc gtctcaatgc tgcctacaat
    15241 cgtcgccccg accagaatct gcgccattgg gccaaattcg gtatcgaaca ggcagatcac
    15301 gcgttcgtta cgggcaaaca gattcggcac gttctgagcg gtgagatggt taacggaaaa
    15361 gagatcgccc ggcacgtaga tcatctcacg cagaataccg ttgcacggca tgtgtacgcg
    15421 gtggtagtca cgtggggaga ggtaagtggt cacaaacgta ccgttgcgga acaggtccgc
    15481 catcagatag ttgccagcca gcagggcttc gaggctgtag ttgtggcctt tggcttgcag
    15541 gattttatct tcttcgattt tacccaactg gctgataacg ccatcggcag gcataaccag
    15601 tacattcgga tcggtatcga ttgggcgtac ttcgtcacgc agcggacgga caaagaattc
    15661 gttaaaggtg cggtagctgg cggtgtccgg cttttgcgcc tctttcatat cgaccttgta
    15721 gtatttaacg aacagatcga taaccagttt tgtcagccat cctgcccgct tgcttgcgcc
    15781 ccaacccgcc aggcgagtaa gccatagttt cggcagaatg tactgtagcg aaagtttaaa
    15841 tgaatttaac aaggtagcct ccaggccatt gttttgtcgt tcctgatccg gcctacatgc
    15901 cggatcctga aaaaaagggg acgattctaa cgacggttag cttaattgtc agtcatccgt
    15961 atcagaaaag tttttacgcg tttttacctg cgccatgctt tccagaatac ggtgatagtt
    16021 ttcgaaacgg gtttccgcga tttttccttc ctcaaccgct tcccggatag cgcagcccgg
    16081 atcggtatcg tgtttgcaat cgcgatattt acacagacct aagtagtcat ggaattcgac
    16141 aaagccctga gtgatttgtt ccggctccag gtgccagagg ccgaactcac gcactcctgg
    16201 ggagtcaatc acatcaccgc cgtgcgggaa gtgatacagc cgagcggcgg tcgtggtgtg
    16261 ctgaccgaga cccgagttgt cagagacatc gttggtcagg atctcttttt gcagacccag
    16321 caatgcgttc agcaggctgg atttgccaac gccagactgc ccggcaaaaa tgctgatgcg
    16381 cccggtcaac gcctcttcca gcggtttcag cccatcctga gtatgactgg aaaccatcaa
    16441 tacgcgataa ccgatattgc ggtagatatc catctgctcg ttgacgaatg ccatgccttc
    16501 gtcgtccagc aggtctatct tgttgagcac aataatcggc tcaatctgca aggtttcgca
    16561 ggccaccagg taacggtcga taatattgag cgacagctcc ggcaaaatgg cggagacaat
    16621 gacaatctgg tcgatgttgg cagcaatagg tttcacaccg tcgtaaaaat ccgggcgcgt
    16681 caacactgag gtgcgctcat gcaccgcttc cacgatccct ttgacattta cgccttccgc
    16741 cgctggttta cccggacgcc agactacgcg gtcgccggtc accaacgaac ggatggtacg
    16801 gcgaatattg cagcggtgaa cgtcgccatc ggcggattcc acatcagcgt gcataccaaa
    16861 gcggctgatg acgatacctt catcaggctc gccaaacaga ttgtcgtcgt agtcgggctt
    16921 ctccttagac gttttaagac gacgctggtg attggcgttc acgcggcgct gctggccttt
    16981 ggagagttta tttttactca atcgtacaga ctcctggtcg cccctgatgg gcaaaacatc
    17041 tatgatacac gcaattgtgg atcaatatag tcactgtgaa tgggtggaaa atagcatgag
    17101 tgccaatgaa aacaacctga tttggatcga tcttgagatg accggtctgg atcccgagcg
    17161 cgatcgcatt attgagattg ccacgctggt gaccgatgcc aacctgaata ttctggcaga
    17221 agggccgacc attgcagtac accagtctga tgaacagctg gcgctgatgg atgactggaa
    17281 cgtgcgcacc cataccgcca gcgggctggt ggagcgcgtg aaagcgagca cgatgggcga
    17341 tcgtgaagct gaactggcaa cgctcgaatt cttaaaacag tgggtgcctg cgggaaaatc
    17401 gccgatttgc ggtaacagca tcggccagga tcgtcgcttc ctgtttaaat atatgccgga
    17461 gctggaagcc tacttccact accgttatct cgatgtcagc accctgaaag agctggcgcg
    17521 ccgctggaag ccggaaattc ttgatggttt taccaagcag gggacgcatc aggcgatgga
    17581 tgatatccgt gagtcggtgg cagaactggc ttactaccgc gagcatttta tcaagctgta
    17641 aaatttttcc tggtcacgta agcgccggaa tccacttgcg gcggcgtgat tttgacgcta
    17701 aattgaacgt cttgctgata atttcagcac ttgagataaa aactcaaaaa aaatttttta
    17761 ggggggttgc agagggaaag atttctcgta taatgcgcct cccgtaacga cgcagaaatg
    17821 cgaaaattac gaaagcaaaa ttaagtagta cgcgggaata gctcagttgg tagagcacga
    17881 ccttgccaag gtcggggtcg cgagttcgag tctcgtttcc cgctccaaaa tttgaaaagt
    17941 gctgcaaagc acagaccacc caatcgcagt atttcgaatt gcgacaaggc ggcaactgaa
    18001 cgaatccttg ggaacttaca aaagtaagtg actaaggtga gtgaaggcag ccaaggcagt
    18061 agcggttcga aagacgaaga ttaagcggga atagctcagt tggtagagca
//
LOCUS       DAZGHF010000094        18079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGHF010000094 DAZGHF010000000
VERSION     DAZGHF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.094"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..4515
                     /locus_tag="ACHK3A_003369"
     CDS             <1..4515
                     /locus_tag="ACHK3A_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO8741414.1"
                     /translation="LDNYFGRYDVSTDGKITSGGDFMTEAQRARRVFKDGRYVDAEYP
                     VRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAARAFERYVQDKARM
                     AGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRTLKTRETDKGVAFY
                     SRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVISGIKDSDLKVRVVKSQKEA
                     EALAGESFDGYGKVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEHVVTPA
                     EYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKFVAA
                     WERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGVGASSQYS
                     RSGKPDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVLRHLGAPDLPLVIS
                     RDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVMLLDAVDKNGDPVV
                     SAVHMKAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKLSRDNLLHRGLQLP
                     KGEHSYRGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQEN
                     IRKTGGKVDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADLDEYL
                     YARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIIDDML
                     ARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRESRQ
                     AMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDT
                     MRVIAERKDQETGETIREVVERPVAMAMMADRYFTTKKNGKTYYIKLHDPRLMRAMKN
                     MGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKL
                     KGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGNGAQWQKVWKEFVEDGGKTGWFNMG
                     DLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHARDAG
                     LSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGDGPLL
                     ERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNLVIM
                     KSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGAFSPV
                     GSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRS
                     TPEAYKAFASWLNAFSGGSQYRPGAVDVTPESLKFWIDYISGGTGRFISKTTDAAVKS
                     LNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTAFIDE
                     NNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSSLSLAQQVAMVKSVERDMKIAVDRFN
                     REYNKKVGVD"
     gene            complement(4605..4678)
                     /locus_tag="ACHK3A_003370"
                     /pseudo
     CDS             complement(4605..4678)
                     /locus_tag="ACHK3A_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813257.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            5447..5620
                     /gene="ymdF"
                     /locus_tag="ACHK3A_003371"
     CDS             5447..5620
                     /gene="ymdF"
                     /locus_tag="ACHK3A_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO8741415.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(5703..7031)
                     /gene="rutG"
                     /locus_tag="ACHK3A_003372"
     CDS             complement(5703..7031)
                     /gene="rutG"
                     /locus_tag="ACHK3A_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO8741416.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGHVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(7052..7546)
                     /gene="rutF"
                     /locus_tag="ACHK3A_003373"
     CDS             complement(7052..7546)
                     /gene="rutF"
                     /locus_tag="ACHK3A_003373"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO8741417.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(7557..8147)
                     /gene="rutE"
                     /locus_tag="ACHK3A_003374"
     CDS             complement(7557..8147)
                     /gene="rutE"
                     /locus_tag="ACHK3A_003374"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO8741418.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(8157..8957)
                     /gene="rutD"
                     /locus_tag="ACHK3A_003375"
     CDS             complement(8157..8957)
                     /gene="rutD"
                     /locus_tag="ACHK3A_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO8741419.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLVQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(8965..9351)
                     /gene="rutC"
                     /locus_tag="ACHK3A_003376"
     CDS             complement(8965..9351)
                     /gene="rutC"
                     /locus_tag="ACHK3A_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO8741420.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(9363..10055)
                     /gene="rutB"
                     /locus_tag="ACHK3A_003377"
     CDS             complement(9363..10055)
                     /gene="rutB"
                     /locus_tag="ACHK3A_003377"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO8741421.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELMPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(10055..11146)
                     /gene="rutA"
                     /locus_tag="ACHK3A_003378"
     CDS             complement(10055..11146)
                     /gene="rutA"
                     /locus_tag="ACHK3A_003378"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO8741422.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDSLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAAHAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSDTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            11434..12072
                     /gene="rutR"
                     /locus_tag="ACHK3A_003379"
     CDS             11434..12072
                     /gene="rutR"
                     /locus_tag="ACHK3A_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO8741423.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(12112..16074)
                     /gene="putA"
                     /locus_tag="ACHK3A_003380"
     CDS             complement(12112..16074)
                     /gene="putA"
                     /locus_tag="ACHK3A_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO8741424.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPPVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGDGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            16129..16338
                     /locus_tag="ACHK3A_003381"
     CDS             16129..16338
                     /locus_tag="ACHK3A_003381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741425.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            16497..18005
                     /gene="putP"
                     /locus_tag="ACHK3A_003382"
     CDS             16497..18005
                     /gene="putP"
                     /locus_tag="ACHK3A_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO8741426.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
BASE COUNT         4322 a         4661 c         5027 g         4069 t
ORIGIN      
        1 ctggataatt attttggtcg ttatgacgtt tccactgacg ggaagattac gtcaggtggc
       61 gactttatga cggaagcaca gcgtgccagg cgcgtattta aagacggcag gtatgttgat
      121 gctgaatatc cggtacgtca ggaggtttac gacgctttta aaggtgtgat tcaggccatt
      181 aaaaacagtg acatgccgcg tcgttcagcg cttctcgatg aggtgcgctc aaaaccgtac
      241 tggtcaacgg atgttgaaat ggcggcacgt gcctttgagc gttatgttca ggataaggcg
      301 cgtatggctg gcgtggagaa tgattatctg gtcaatatcc gtaaggcacc tgagcacaac
      361 acagataaca cctacgctta tccgacgaat gcggaactgg atggcggtat tcgtgaggca
      421 ttcgatcacc tgttccgcac cctgaaaacc cgtgagacgg acaagggagt tgcgttttat
      481 tcccgtaagg gcgtgacccg cacacctgaa ggcaatctga tttcggatgt taaccgcagt
      541 gcggaagcca aaggcagtcc ggtcccgcag gttgaagctg ttgcccgtgg cgtgattagt
      601 ggcattaagg acagtgacct gaaggtccgc gtggtgaagt cacagaaaga ggctgaagca
      661 ctggcgggtg aatcattcga cggttacggc aaggtgcacg cgttctatcg cccggataaa
      721 cgagaaattg tcctggtggc ggataacatc cctgacgggc ggaccgttcg cgagaagtta
      781 cgtcacgaga ttattcacca tgcaatggag catgtagtca cgccagcgga atatcagacg
      841 attattaaga ccgtgctgaa aacccgtgac agtgataacg ccaccatccg tgaagcctgg
      901 cgtaaggttg atgcgtctta tggtaaggaa tcaccggaag tgcaggcggg tgaatttctg
      961 gcgcatatgg cagagaagca gccgaataaa ttcgtggcgg catgggagcg tgttgttgcc
     1021 ctggtcaaag gggtactgcg tcgtacgggg ttactgaagc cgacggaact gaacgatatc
     1081 agacttgttc gcgaaactat tcgcacgtta ggccagcgtg tgcgggaagg ttacacgccg
     1141 cgtgaggatg gcgtgggcgc atcgtctcag tactcccgta gtggtaagcc tgatccgttc
     1201 aaagtgccgg agggtgaggg tgaacgttat cgcgatgacc ttgccagaat gatgaaatct
     1261 ctgcgtacca cagatttaac ggtaaacatc gggcgtacgc cgccggtatt gcgtcacctt
     1321 ggcgcaccgg atttgccgct ggttatttcc cgcgatactg tgcggaaggc caccaatggt
     1381 gtgaaacatg tggtgccgat ggatgttatc gagagactac cggaactgat gcacgatccg
     1441 gatgcaattt accgctcagc gacagaaaga aatgcggttg tgatgctgct tgatgccgtg
     1501 gataaaaatg gtgatccggt ggtgtcagcg gtacacatga aggctgtccg gtcgcgtctg
     1561 gaaatcaaca aggtagcttc tgtttacggt acagaaaatg gaaaaaaact gaagagtatg
     1621 gaaatgaccg ggttaacgtt gtaccggaga gaaaaattaa gccgcgataa ccttctgcac
     1681 agagggctcc aattgcccaa aggggaacat tcttatcgcg gctctgcgga taaaatactc
     1741 tatcctgaag atattcgcaa ggggccgtat tactcccgta ccagcagtct gacaccggaa
     1801 gagacaattg catcgcgttt tgtgcgccag atgcaggata aattccaggt gctgaaagct
     1861 gttcaggaga atatccgtaa aactggcggc aaagtggacg acagtaacaa cgcttatatg
     1921 gcggaagaac tcttccacgg gaaggcggaa aacgacctga acgtgatgaa ggagcgctac
     1981 gttcagccac tggctaaatt actggcggac tacaaaattg cgcaggccga tctggatgag
     2041 tacctctacg cccgtcacgc gccggaacgt aacgcgcata tcgcgaaaat caacccgaaa
     2101 atgccggacg gcggttcggg gatgaccaac gcggaagcgg cggaaatcat gcagcgtgta
     2161 cgtaacagtg gcaaacaggc acagtatgac cgtctggcag ggattattga cgatatgctg
     2221 gcccgtcgcc gtgagcttat ccgtgaggcc ggacttgaag agaacggtgt ggtggatgcc
     2281 tggcagaacg cctaccgtta ctacgttccc ctgaaaggtc aggatgttga cggtgtggtg
     2341 tcactgcccc gtacaggtaa gggcttcacc atcggcggac gtgaaagcag gcaggccatg
     2401 gggcgtgcat cccgggcaca gtctccgtcc actcaggcga tacaggacct gagcgaatcg
     2461 ctgatccgcc atcgcaaaaa tgaagtgggt aacgccttcc tgaagctggt gcaggataat
     2521 cccgataagg attactggca ggtattcact gatgacagac cggataccat gcgtgtgatt
     2581 gcagagcgca aggaccagga aactggtgaa accattcgcg aagttgtcga gcgtccggtg
     2641 gcgatggcaa tgatggcaga ccggtacttc accaccaaaa agaacggcaa aacgtactac
     2701 atcaaactcc atgatccgcg cctgatgcgt gcgatgaaga atatgggacc ggaaaccagt
     2761 aacgccgtaa tccgcacgct ggggaaagtt aaccgcttcc tggcaacggt gaacacgtct
     2821 tataacccgg aattcctggt cagtaacttc atccgtgacg ttcagacggc ggtgatgaac
     2881 ctgaaggcgg agcagggaag gagcgacggc aaactgaaag ggctggataa cttatccgca
     2941 ctggctgtgg tgaaagacag ccggtctgcc atgtctgccg tatacgccag tctgcgtggt
     3001 aaaaccctca cgggcaacgg tgcgcagtgg cagaaggtgt ggaaagagtt tgttgaggac
     3061 ggaggtaaaa ccggctggtt taacatgggt gaccttgaag gccagcagaa ggaaatggat
     3121 cgccttgtat cgctggcgaa gggaggatgg aaaggccaga gtatcggtgc atggaattcg
     3181 ttccttaacc ttgtcgagga tgccaacggg gcggttgaaa acgctctgcg tctttctgcc
     3241 tataaacacg cccgtgatgc cggtttgtca cgccagcagg cggcgtctct tgccaaaaac
     3301 atgacggtga actttaaccg tcgtggtgag cagggagcgc tgatgaactc gctgtacatg
     3361 ttcgccaacg ccagcattca ggggaccgca aacctggtga gaacgctcgg acatcttaat
     3421 ggcgacgggc cgttactgga gcgccttcgc tggaagaatc tcaatgtacc gcagaaaatc
     3481 gcgcttgcag ctgtgggagc gggttatctg cttggctcgc ttaaccgcag tgttgcgggg
     3541 gaggatgatg acggggttaa ctggtatgac aaggtgccgt ctcatgtgaa agagcgtaac
     3601 ctcgtcatta tgaaatcggt gttcgggggc aaggccggag agtactggag tattcctctg
     3661 ccttacgggt acaacgtttt cttcctgctt ggtcacacag ccgaaggggt ggcagcgggc
     3721 gacctgaccg cttcacgtgc tgccggtaat gttgttggtg gtgtgcttgg ggcattcagt
     3781 cctgtgggca gtgaaacgtc ggaaacactg tccggggcat tgctgaaaaa tgcagcgccg
     3841 accattctgc gtccgtttgc gaaccttgcc atgaatgaaa acttcatggg ggcgcagatt
     3901 taccaggaga acatgccgtt tggtacacca aaacctgaca gtcagctggg aagacgttca
     3961 acgccagaag cgtacaaggc gtttgcatcc tggctgaatg cgttctcagg tggcagccag
     4021 taccgtcccg gcgcggtgga tgttacgccg gaatcactga aattctggat tgactatatc
     4081 tctggcggga ccgggcgctt catttccaaa accacggatg cggcggtgaa atcgctgaat
     4141 ggtattgata taccggagca gcaggtgcct ttcctgggga aaatttcagg tgaggtgatg
     4201 ccgtatgcag accagcagaa gatgtacgac cggatgacgg aggttgcgca gtaccacgca
     4261 gagctgaaga gtctgaccgg tgcagaaaga acggcgttca ttgacgagaa caacggaaaa
     4321 ttgtcgatga acgggcttat gcaggatacc cggaagagac tgaaggattt gcgtaaacag
     4381 cgcgatgcca tttatgccga cagttctctc agtctggcgc agcaggtggc gatggtgaaa
     4441 tcggtagagc gggatatgaa aattgccgtg gatcggttta accgcgagta caacaaaaaa
     4501 gtgggagtgg attaacagaa atggccccgt acggaagtgc ggggctaatg tgattgggga
     4561 gggtgaggat acctgacaca tcagaggtgg cgagggattc ctcccttgca gaggtctttc
     4621 ttcgttatca gtgccgtcac aatgacggta atacagagat gagcagggtg attaacatcg
     4681 ccttttgctg ctttcattgc atttctcccg cgatagctca ggcgtatttg tcaatatgca
     4741 gaaatattga tgaacgaatg acggtaaata ccctacccag aaaattaacg taaattcaca
     4801 tcgtaaactt gcgttaatta tctgaaatat atacttaaat ccccttaaat ctttggggcg
     4861 ctattggggc aaagtgtggt tgtttggggc atatttgggg caaaaaagaa cgtataaaga
     4921 acggtgtaaa atcgatttct ttatggtcaa aatgaggtct aacatattga aaagtatatg
     4981 ctcttggacg atcttcggta attctgtgtt tttaatgctt catggtttca atatgtccgt
     5041 acatggaata ataaagcacc agaactttag ccatttctaa ccactcctcg tgttatctct
     5101 attccgtagc gattcgctac cacttattta aagataagac gtccttttca gagtgcaaat
     5161 ttcacaacca cttatttgat ttataacaac tttcacaagc acgtaatttt gtcgcaaaat
     5221 gacacatttt tatctcatcg cgttttttta atcataagag cggcttatgg ataattattg
     5281 gagatgatat ctattctcgc taagaagctg ttgcaggata ttactaaacg cgggtctgcc
     5341 cgcgtcagtt cactaagctt agtcccacgt agcgaaaata tggcagccgc catacgccgc
     5401 gttaattcta tgcaatatga tgtctatacc cagacggagg tcagtaatgg caaaccatcg
     5461 aggcggttcc ggcaattttg cagaagaccg cgaaagagca tcagaagcag gtaaaaaagg
     5521 tggacagcac agcgggggta atttcaaaaa tgatccgcag cgcgcatctg aagcaggtaa
     5581 aaaaggaggc aagagcagtc acggtaaaag cgacaactag ccgggctaat ccatgacgaa
     5641 tgtatttttg tctgaaactc gtcaaaaagt catcaccgcc ggttaccccg gcggttttct
     5701 atttatggct cctgatgaac aacttctggc ggtggaacgt caaccaacct tctgcttaac
     5761 aacgcattga gtaaaatcgc gccaaaggtg gctgtaccga tccctcccaa cgtaaaaccg
     5821 cccagtgtga gagcaaaatc acccgcaccc agcactaagg ttactgcgac cataatcaaa
     5881 ttaccgttct ggcttaaatc cacactgttt tgtacccaga tccgtgcgcc tgcgacggca
     5941 atcagcccga acacaacaat tgatgcacca ccaatcaccg cggccggaat agtatggatc
     6001 agcgcgccaa atttcggcga aaagcccaac agcatggcga tgacggcagc ggcaacaaac
     6061 accagcgtcg agtagacttt tgtcacggcc atcacaccaa tattctcggc gtaggtggtt
     6121 acgccgctac cgccgacaga gccagaaagc atcgtcgcca gcccatcgcc tacgaatgcc
     6181 cgccccatat acgggtccat attgcgtccg gtcatcccgg cgaccgcctt gagatgacct
     6241 aagttttccg ccaccagaat caccgccacg ggcgcaatca gcatcattgc ctgaccatta
     6301 aaagcaggag tggaaaaatg tggcagaccg aaccaggcag catggctgac gagagtaaaa
     6361 tcgacggctt ttcccagccc taaaacgttg gtcatcacgc catacagcag acaggcgaca
     6421 attaatccta cgagaatcaa taatcgctgg atcatgccac gggtaaatac ggccaccagc
     6481 ccaatacaca gcaccgtcat taccgccatc cagctatcaa aggccgaagc cgatacattt
     6541 ttcactgcga taggcgctaa gttcaggcca atcgccatca ctaccgcacc tgtcacaacc
     6601 ggtggcatta gtcgttcaat ccagcgcgta ccgattttca tcaccaccag gccaatgacg
     6661 gtataaacca gcccacaggc gataatcccg cccagcgcga tgctgatatt cggattaatt
     6721 ccctgaccat taaagcccgt cgcggcaatc accacgccga caaaagccgc gctggaaccg
     6781 agataactgg gcacgtgccc gccggtaata aagaaaaaca gtaacgtgcc gatccccgac
     6841 attaaaatgg aaagattggg atccagcccc atcagaattg gcattaacac cgtcgcgcca
     6901 aacatcgcca ccgcgtgttg aacgcccatt actgccgttt gagcaaacgg caatcgttca
     6961 tccggcgcga ccacgccgct ctctgtagag gtcgatttta actgccagtg aggaaaaccg
     7021 aacattgcca tcagctgtct ccttaaggag gttaacaagc agggcgcatc agcgcgtgat
     7081 aactgcgatc gaaccacacc agcccgtagg gtgtggcgtg acgatgaatc gcttcgatgg
     7141 cgcaaaacaa aatgtcgtgg gtgccgacgc tcaccacctg gctgatacgg cagtcaaacg
     7201 aaaccagtgc ctcttccagt tgcgggcatc cggtcacacc agtctgccag cgggcggcgg
     7261 caaagcggtg ttccatgggc gttttgccgc caaaaaggtt tgaaagcggc tcctgcccgg
     7321 cgctaagtgt atttacacac agcgttcgat tttcattgaa taccggccag acggacgccc
     7381 cacgattcag gcacaccagt aatgtgggcg gcgtgtcagt cacgctgcag acggcgctgg
     7441 cggtgaaccc ggcgcgcccg gctggaccgt ccgtagtgat gatattgacc gccgcgccca
     7501 tacaggacat cgcatcgcga aaggtttgtt gatcgacaat gttcattgtt tgctccttac
     7561 aacagcccgc aggcttcttc aaaggacaga cgtggcaggc gcgcaaaaag tttgctggaa
     7621 tcgccatagc cgatattaat cagcagattg ctcttcagcg tgctgcccgc aaaaaaggcg
     7681 tcgtcaacgt attgacggtc aaagcccgac atcgggccgg tatccagccc cagcgcacgg
     7741 caggcgacga tcagataggc cgcctgcatg gaactgttgc gaaacgctgt ttcttcggca
     7801 agttgtggac tggaggtaaa ccaactgcgg gcatcgccgt gaggaaacag ctgtggtaac
     7861 cgttcataaa attcactgtc ccaggcgacg atagcggtga cgggcgcggt cagggttttt
     7921 tgcagattgc cgctggaaag cgccgggcgc agacgttctt ttccttctgc agtgcggata
     7981 aacacgatcc gtgccggaga acagttagct gatgtcggcc cccatttcat cagggcataa
     8041 atctcccgta acgtctcatc gctgacgggt gtctcccgcc agccgttgtg agtgcgggca
     8101 tcggtgaaca gggtgctaag cgcacctggg ctaacggctt cgttcatagc aattccttac
     8161 agagcggctt cacggtgatg taacaggctg gcaagcccgt tgagtaacag agcattaaac
     8221 gtttcgggat cggtcacgtt gcaggcgtgt ccgccatagc gcatcaccat tttctggcta
     8281 tcgggcaggg cggcatgaag ttcactggaa catgctgttg gcaccagcag atcatcactg
     8341 gcgcagatga tttgcaccgg gcagcggata cgatccgcat ggtgactaaa gtcagcgcgt
     8401 ttgagggcgt taagtcgacg cagtaaatta tttttgcctt gaaaatgcgc cagtgccagc
     8461 gcgtcttctg cctcaaggcg aggtgcgcgg gccgccatcc agtcggcggg atagaggaac
     8521 aacggctgcg cttccaccca tgcctgcgcg ccgccgctat gtagtaactg ttcgcgaacc
     8581 tgaaaacagc ggcgcgtatg ggcgtttatt cgtagccagc cgttaacgct gaccagcaca
     8641 gttaccgacg cgggataatc cagcgccagc tgcattccca ccagcgcacc gagcgcatgg
     8701 ccgaccaccg cgtaacgctc aatccctgcg gctaccagcg cctgatgcag ttccgctgcc
     8761 atctgggcga tactgtaatc ttctgccagc gtgtcgggat tattgccggt gccgcgctgg
     8821 tcgtaacaga ctacctgata ctcctgaacc aacaccgcca gttgcggtaa ccagtaactg
     8881 ccgctaccgc caagacccga aatcaacacc actacgggcg catcagcata agggggaggt
     8941 gagagtgaaa gtttcatcgc ggcctcactt ggcgatatgc gcaattgtgg cgatttccac
     9001 cagcgcgtca ggttttacca gtccgcactg aatgcagaat cgcgccggtt tgtcacccgg
     9061 gaaaaattcg gcgtagattt cgttaatcgc ggcgtaattt ttccagtcgg taataaagat
     9121 gctgttgaag gtcacatccg ccattgtgcc acccgccgtc tcgatgacct tgcggatggt
     9181 ttccagaacg tggcgggttt gcgcctttgg gtcatcggca aacagcacgt tgttatgttg
     9241 atcaaaagcc agcgtaccgg agacatacac cacgccatca gccagcgtgc cgggaacgaa
     9301 gggggccagc ggtgcgctgc tgccagcagg aataattacg gattttggca tcgttaaact
     9361 ccttaagcga tacgagcaaa ggacgtggaa gaaagcgcgt cgcagaacgt ttcgacgtcg
     9421 ctgacccagc caaaaaaggt ttcgatattg aacaacgcgg ctttctgtgc aaattccggt
     9481 cccgcctggt gtgttgcgtc ttccagcacc acgccgaaat actccagaaa aaagccgtcg
     9541 cgtagcgtcg attcgacgca gacgttggta gcgataccgg tgaaaaccag atggcgtatt
     9601 ccacggctgc gcaaaatgct gtccagcggc gtattgaaga aaccgctgta gcgcggcttc
     9661 ggcagcacaa tatcgccagg ctgcggcatc agttcatcca ccagttgata atcccaggag
     9721 cctttcgcca gcaatttccc ctgcagctgc ggctgcttac gcatggtttt cagggcgttc
     9781 gatttatgaa aattcggtga gccggggccg ccagcctcga catactgttc atcccagcca
     9841 ttttgaaacc agatgatcaa catccctgcc gctcgcgctg cggtcacggc ggtttgaatg
     9901 ttggcaatga ccgggcgagt ggttgagaca tcaaacccgg cgagatctaa gtagccgcct
     9961 ggcgtggcat aagcgttttg catatccacc acgatcagcg cagtttgctg cggatcgaag
    10021 gtaatggctt ccggtcgtgc ggttaaggtc gtcatcatgc cacctcctga gtcagcgcag
    10081 ggagatgggc gcggcactgc atcagtggtt gaatgcgctc gccgaagttt tcgattcccg
    10141 acagaaaatc atcgaaggtc aacagcacgc cttcggcacc aggcacgctt gcgacttcat
    10201 ctaacatgcg cgcgacactg gcgtaagaac cgactaacgt ccccatattg atgtttaccg
    10261 ccgaagtggg atcggccatc tgacggacgt tggtgtcagt atcggagcgg gtatctttct
    10321 gactttgttc ggttagccag cttaacgcct cttcatccgc gcccgctttg tagtgttccc
    10381 attttgcatg agcggcatcg tcggtttcgt cggcaatcac cataaacaac acataagagc
    10441 caacgtcgcg tccggtttgc tctgcggcct gtttcatccg cgcagcggtc ggggcgaaag
    10501 ccgtgggtgt atttacgcct ttgccgaaac agaagttaaa atcggcatac cgggcggaga
    10561 acgccatgcc agcgtcgctt tgcccggcgc agatcacttt catggggaca ctcggttgcg
    10621 gactgacgcg acaatcatcc atagtgaaaa aatcgccttt aaaatcgctt ttccccgagc
    10681 cccacaggtc gcgcagcacc tgaacatatt cggtgagaga gtcgtaacga cgggagaaat
    10741 agtcatcgcc aggccagatc cccatctgct catactcggg cttctgccag ccagtcacga
    10801 ggttgacgcc aaaacgcccg ccagagatgg agtcgatggt tgcggccata cgggcgacga
    10861 ttgccggagg taacgttaag gtggcagctg tcgcgtatat ctgaatgcgt gaggtgacgg
    10921 ctgccagccc cgccatcaag gtgaacgact caaggttgtg atcccagaac tcagttttgc
    10981 cgccaaagcc acgcagtttg atcatcgaca gggcgaaatc gaaatggtag tgctccgctt
    11041 tttgcacaat ggctttattc agttcaaatg tcggcatgta ctgcggcgcg tgggtcgaaa
    11101 tgagccagcc gttgttgcca ataggtacga atacgccaat tttcatcatc aacctctctt
    11161 cgtctcgtaa agtgaaagtc agacggggcg ctgcatcctg catatccttt tcagccgcgt
    11221 aatggcttgt ttgcaaagcg gatgccagtt tttaaaaagt gaatgttatt aatgtgttaa
    11281 tgttaagttg tctaaaatgc ctgaaaaaaa gtggactaaa cggtcaaaac agttgcacat
    11341 aaaacatgca ttcgtgcgcg atgagagtgc agaaggtcga ggccgggcgg gggttttgct
    11401 atcctgttgc caatcaacaa gaggggagag cgcatgacgc aaggcgcagt gaaaacaacg
    11461 ggtaagcgtt cgcgcgcagt aagcgcgaag aaaaaagcga ttcttagcgc agcactggac
    11521 actttttcac aattcggttt tcacggcaca aggctggagc agatcgcgga gttggcgggt
    11581 gtttcaaaaa ccaatctgct gtattacttt ccgtcaaaag aggcgctgta tatagccgta
    11641 ctgcggcaga ttctcgatat ctggctggca ccgttaaaag cgtttcgaga agatttcgcc
    11701 ccgctggcgg cgatcaaaga atacatccgt ctgaagctgg aagtttcccg cgattatccg
    11761 caggcttcgc gcctgttctg tatggagatg ctggcaggtg caccgctgtt aatggatgaa
    11821 ctgacgggcg atttgaaggc attaattgat gagaaatcgg cgctcattgc cggttgggtt
    11881 aaaagcggca aactcgcgcc gattgacccg cagcatttga tttttatgat ttgggcttcc
    11941 actcaacatt acgccgattt tgcccctcag gtggaggcgg tgacaggcgc gacgttgcgc
    12001 gatgaggtgt ttttcaatca aacggttgaa aacgtgcagc ggattattat tgaggggatt
    12061 cgaccacgtt aaagatgccg gaggaggttg taacatcctc cggctacctg tttaaccgat
    12121 cgtcattaaa ctggcgttac cgccagcagc agcggtattc acactcagcg aacgctcgat
    12181 atacagccgt tccagaagga tattgctttc gccacgggca aaaccctgca ccgaaacaat
    12241 tgcaccatcc cgcgcggcaa cggcttcaca caatgcgcga agctgatccg aatcaccgtg
    12301 gaagatcacc gcatcaaatg gttgagcggt tatattttcc gctttcgcca gttgaatacg
    12361 ttcgctgact gccgatggca atgccttcac taactgacga tgcagcgcat catccggcca
    12421 cagcacctgg ctacccaccg ccagcacggc ggcgagctga gtcagcgcat cctgttcatc
    12481 atccgcaata cacaacacgc gctcacgcgg cagcagcgtc caggtgttgc gttcacccgt
    12541 cggccctggc aacaatcgtt gtgttcctgc ctgcgccaac tcgccatatt gcgtacataa
    12601 cgcctgcaat tctggacggt ttgccgccca ttcccgcagt gcatttagcg gctgagtcaa
    12661 tgcggctttc aactgcgcat cgaccggata ctccgcatcc tgacgcgcga gcgtcactgc
    12721 cagcgcactt tccgggcgat tcgccagcag acggtagaga tagagcggac cgcctgcttt
    12781 cggcccggta ccggacaacc cttcgccgcc gaacggttgc accccaacca ctgcgcccac
    12841 catattacgg ttaacgtaca ggttaccgac atgcgccgag ccagtgacct gggcgatggt
    12901 ttcatcaatg cgcgtatgga cgccaagcgt cagaccataa ccggaagcgt taatctgctc
    12961 gatcagcgcc ggcagctggt tacggttgta acgcaccaca tgcaacaccg gaccaaagac
    13021 ctctttttgt aattcggcaa agtcatccag ttcgatcagc gtcggagcga caaaggtgcc
    13081 gctttgccat tcacgggcat cttcgctgtt ttcccgcacc gcctggaaca cctgacggcc
    13141 tttactacgc atggtctgaa tatgacgctc aatattggct ttcgcttcgc tatcaatcac
    13201 cggaccgata tcggtggtca ggcgacccgg attacccatc cggcattcgg ccattgcgcc
    13261 gcgcagcatt ttcagcgtgt ggtcggcaat ctcatcttgc aggcacagca cgcgcagtgc
    13321 cgaacaacgc tgacctgcac tgtcgaacgc cgaggccagc acatctatta cgacttgttc
    13381 ggtcagtgct gaagaatcga caatcatcgc gttcatgccg ccggtttcag cgatgagcgg
    13441 aatagggcga ccctgagcgt ccaggcggct ggcgatattg cgctgcagta acgtagcgac
    13501 ttcggttgaa ccggtaaaca tcaccccgcg cacgcgatca tcacccgtca gttgcgcgcc
    13561 cacggtttca ccccgacctg gcagcaattg caccacgcct ggcggtacac ccgcttccag
    13621 caaaatggcg atcccttgcg cggcaatcag cggcgtttgt tctgccggtt ttgccagcac
    13681 gctgttacct gccgccagtg cggcagcgat ctgcccggtg aaaatagcca gcgggaagtt
    13741 ccacggactg atacatacca caggccctaa tggacggtgg gtttcgttag cgaaatcatc
    13801 ccgcacctgt ccggcatagt agtggagaaa atcgaccgct tcgcgcactt cggcaatggc
    13861 gttactgaag gtttttccgg cttcacgcac cagaatacca atcagttgct gcatctggct
    13921 ttccattagc acggcagcgc gatgcaaaat cgccgcgcgt tcagccggag gagtggcaaa
    13981 ccagattggc gcgttattaa ccgcactttc cagcgcctgt tctacttcac gcggcgtggc
    14041 ttcacgcaca aagcccacaa tatctttcgg ttccgcaggg ttaataacgg gcgacatctc
    14101 accagccgcg accgattgtt ccagcattgg caaggcctgc catttttgca gtgcactatt
    14161 gagcagggca gaagagagcg aggccagccg gtgttcattg gcgagatcca gccctgccga
    14221 gttgtcgcgc ccgtcaccgt aaagatcgcg cggcagggga attttcggat gcggtaatcc
    14281 agtttgccct tcctgttgcg ccagtttttc tacagcagtg accggatcgg caaccagttc
    14341 atccagtggc aaagaggtgt cagcaatacg gttaacaaac gaggtgttag caccgttttc
    14401 cagcaggcga cgcaccagat acgccagcag cgtttcatgg gtaccgacag gagcataaat
    14461 acgacacgga cggttaagtt tgccgtcggc aactttcccg gtgacctgct catacagtgg
    14521 ctcgcccata ccgtgcaggc actggaactc gtactgaccc gggtagtagt tctgacccgc
    14581 cagttgataa attgccgcca gcgtatgggc gttatgcgtt gcgaactgcg ggtagattag
    14641 attcggcacc gccagcagct ttttcgcaca ggcgagatat gaaacgtcgg tatacacctt
    14701 gcgggtataa accggataac cttcaaggcc gtccatctgc gcgcgcttga tttcactatc
    14761 ccagtacgcg cctttcacca ggcgaatcat cagacggcga cggctgcggg tggcgagatc
    14821 aatcaggtaa tcaatcaccg gtgggcagcg tttttgataa gcctgaataa caaaaccgat
    14881 gccgttccag cctgccagtt ccggctcgaa acagagtttt tccagcagat cgagggagat
    14941 ctccagacga tcggcctctt cggcatcgat gttaatacca atatcgtact gacgcgccag
    15001 cagggtgagt gatttcagac gtgggtaaag ctcttccatt acccggtcat actgggcgcg
    15061 gctgtaacgc ggatgcagcg ccgacagctt gattgaaatg cccggacctt catagatgcc
    15121 acgaccattg gacgctttac caatggcgtg aatcgcctgc tgataggaaa ccatatacgc
    15181 ctgtgcatct gcggcggtca gcgcggcttc gcccagcata tcgtaggagt aacggaaacc
    15241 tttctcttcc agcttgcgtg cattggctaa cgcttctgca atggtttcac ccgtgacgaa
    15301 ctgctcgccc atcaggcgca tcgccatatc cacacctttg cggatcagcg gttcaccgct
    15361 cttaccgata atgcggttca gcgagcggga gaggctggct tcgttatggg tggaaaccag
    15421 tttgccggta aacagcagcc cccaggtggc ggcattaaca aacaacgacg ggctacgacc
    15481 aatgtgtgac tgccagttac cgttgctgat tttgtcgcga attaacgcat cacgggtggc
    15541 tttgtcggga atacgcaaca acgcttctgc cagacacatc agcgccacgc cttcctgcga
    15601 tgacaacgaa aactcctgca ataacccctg aaccatacct gcgcgaccac tggcgttttt
    15661 ttgattacgc agtttatcgg ccagttgata cgccagtttg tgcgcctgtt cagcaactgg
    15721 ctgcggcagg cgagcttgtt ccagcagcat cgaaaccgct tcggtttccg gacggcgata
    15781 ggcagcggtg atcgcggcgc gggaaaccga ctggggcaaa atttgctcgg caaagtcgag
    15841 gaaaggctgg tgtggttcct ctgccggagt cggtgcttca tcgctctcat tggcagcgcc
    15901 agaaagcagc gcaggtagct ccggcagagt atcgctgttt tccagttgtt caagataaga
    15961 aaaaatcgcc tgtttaatta accagtgtgg cgtgcgatcg atacgtgtcg cggcagactt
    16021 aatacgctca cgcgtcgcgt cgtccagctt aacccccatg gtggtggttc ccatgccatt
    16081 actcctgttg ttctgaaagg tgcaacttaa cgttatcgtg aaatatccat gatgttgcaa
    16141 ctttgtgcaa ctgtgttaaa tgtgacctgc gtagcaagct taaaaatgaa tgaaatgtta
    16201 ataaacgaaa tcgatatgac agggattaaa aaaataactc agactttttc tctgcggcag
    16261 ttaacatttt tgaaaggtgc aaccgcaaaa aatgtgagag agtgcaacct gatgaaaaat
    16321 agtgtcgctg agcactaaaa tttaatgtaa atggtgtgtt aaatcgattg tgaataacca
    16381 gcgcttccgg caggatacgg tcgccctggt aaaacataaa ctctgttacc ccgttccggt
    16441 ggcagatata acggcaagtt tcgacattgc cgataataat tttttggaga ctttagatgg
    16501 ctattagcac accgatgttg gtgacatttt gtgtctatat ctttggcatg atattgattg
    16561 ggtttatcgc ctggcgatca acgaaaaact tcgacgacta tattctgggc ggtcgtagtc
    16621 ttgggccatt cgtgacggca ttatcggcgg gagcgtcgga tatgagcggc tggctgttaa
    16681 tggggttgcc gggcgcggtt tttctttccg ggatttccga aagctggatc gccattggcc
    16741 tgacattagg cgcgtggata aactggaagc tggtggccgg gcggttgcgt gtgcataccg
    16801 aatacaacaa taacgcctta acattgccgg attatttcac cgggcgcttt gaagataaaa
    16861 gccgcatttt gcgcattatc tccgcgctgg ttattttgct gttcttcacc atttattgcg
    16921 cttcgggcat tgtggcaggc gcgcgtctgt ttgaaagtac ctttggcatg agctacgaaa
    16981 cggctctgtg ggcgggggct gcggcgacga tcctttacac ctttattggc ggtttcctcg
    17041 cggtgagctg gactgacact gtacaggcca gcctgatgat ttttgccctg atcctgacgc
    17101 cggttatcgt gatcattagc gtcggtgggt ttggcgactc gctggaagta atcaaacaaa
    17161 agagcatcga aaacgtggat atgctcaaag ggttgaattt tgtcgccatt atctcgctga
    17221 tggggtgggg gctgggttac ttcgggcagc cgcacatcct ggcgcgtttt atggcggcag
    17281 attctcacca cagcattgtc catgcgcgtc gtatcagtat gacctggatg atcctctgcc
    17341 tggcaggggc ggtggctgtc ggcttctttg gtatcgctta ctttaacgag cacccggcgg
    17401 tagcaggtgc ggtaaaccag aacgctgaac gcgtgtttat tgaactggcg caaattctgt
    17461 ttaacccgtg gattgccggg attttgctgt cggcgattct ggcggcggta atgtcaacgt
    17521 taagttgcca gctgctggta tgttccagtg cgattaccga agatttatac aaagcgtttc
    17581 tgcgtaaaca ggccagccag aaagagctgg tgtgggtagg gcgagtgatg gtgctggtgg
    17641 tggcgctggt ggcgattgcg ctggcggcaa acccggaaaa ccgcgtgctg ggcttagtga
    17701 gctacgcgtg ggcaggcttt ggcgcagcgt ttggtccggt ggtgctgttc tcggtgatgt
    17761 ggtcacgcat gacgcgcaac ggtgcgctgg cggggatgat catcggtgcg ctgacggtta
    17821 tcgtctggaa acagttcggc tggctgggtc tgtacgaaat tattccgggc tttatcttcg
    17881 gcagtatcgg gattgtagtg tttagtttgc tgggtaaagc gccgtcagcg gcgatgcaaa
    17941 aacgctttgc cgaggccgat gcgcactatc attcggctcc gccgtcacgg ttgcaggaag
    18001 ggtaagggac ttagcctgcg gcggttttgt ttggcttcag cagcgggttg cgctccctta
    18061 atgtgcctcg ccatataaa
//
LOCUS       DAZGHF010000095        18055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGHF010000095 DAZGHF010000000
VERSION     DAZGHF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.095"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(45..698)
                     /gene="fdnI"
                     /locus_tag="ACHK3A_003383"
     CDS             complement(45..698)
                     /gene="fdnI"
                     /locus_tag="ACHK3A_003383"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO8741427.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(691..1575)
                     /gene="fdnH"
                     /locus_tag="ACHK3A_003384"
     CDS             complement(691..1575)
                     /gene="fdnH"
                     /locus_tag="ACHK3A_003384"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO8741428.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVNLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEEHHE"
     gene            complement(1588..4635)
                     /gene="fdnG"
                     /locus_tag="ACHK3A_003385"
     CDS             complement(1588..4635)
                     /gene="fdnG"
                     /locus_tag="ACHK3A_003385"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4048..4050),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO8741429.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4867..5748
                     /gene="yddG"
                     /locus_tag="ACHK3A_003386"
     CDS             4867..5748
                     /gene="yddG"
                     /locus_tag="ACHK3A_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO8741430.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LSFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            6021..7409
                     /gene="narU"
                     /locus_tag="ACHK3A_003387"
     CDS             6021..7409
                     /gene="narU"
                     /locus_tag="ACHK3A_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO8741431.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            7504..11244
                     /gene="narZ"
                     /locus_tag="ACHK3A_003388"
     CDS             7504..11244
                     /gene="narZ"
                     /locus_tag="ACHK3A_003388"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO8741432.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELLLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVF
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGPTEGIQPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDMVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLAQHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            11241..12785
                     /gene="narH"
                     /locus_tag="ACHK3A_003389"
     CDS             11241..12785
                     /gene="narH"
                     /locus_tag="ACHK3A_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO8741433.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLNVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            12785..13480
                     /gene="narW"
                     /locus_tag="ACHK3A_003390"
     CDS             12785..13480
                     /gene="narW"
                     /locus_tag="ACHK3A_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO8741434.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            13477..14157
                     /gene="narI"
                     /locus_tag="ACHK3A_003391"
     CDS             13477..14157
                     /gene="narI"
                     /locus_tag="ACHK3A_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO8741435.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            14237..15130
                     /gene="yddE"
                     /locus_tag="ACHK3A_003392"
     CDS             14237..15130
                     /gene="yddE"
                     /locus_tag="ACHK3A_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO8741436.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFESPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPDKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGVAVILFHAEWAIEL"
     gene            complement(15226..16071)
                     /gene="nhoA"
                     /locus_tag="ACHK3A_003393"
     CDS             complement(15226..16071)
                     /gene="nhoA"
                     /locus_tag="ACHK3A_003393"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO8741437.1"
                     /translation="MTPILNHYFARINWSGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            16246..16815
                     /gene="yddH"
                     /locus_tag="ACHK3A_003394"
     CDS             16246..16815
                     /gene="yddH"
                     /locus_tag="ACHK3A_003394"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO8741438.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFYCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(16969..17349)
                     /locus_tag="ACHK3A_003395"
     CDS             complement(16969..17349)
                     /locus_tag="ACHK3A_003395"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8741439.1"
                     /translation="MIIRPVKPSDVEPLLDMLLASGQFNQDGLHHVQKTLTHYFSGQS
                     ADLWFSAEHLGLAGIAYCAPEVMTNDVWNLLMHWISPSHQRQGVGHLLINEIEKELTI
                     KQARLLLVETSSYVDFSAARAFYT"
     gene            complement(17931..>18055)
                     /locus_tag="ACHK3A_003396"
     CDS             complement(17931..>18055)
                     /locus_tag="ACHK3A_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO8741440.1"
                     /translation="IAINILTNDKVFKAGLRRKMRKAAMDKNYLASVLAGSGLS"
BASE COUNT         4297 a         4726 c         4557 g         4475 t
ORIGIN      
        1 ggcgcttctt aaagcgccat tttcagctta aagttcaaaa gagattatat cccttcttca
       61 ctctcttttt tcgcttccgc cttctcgatt tcacgatacc agcgcggatg gtgtttcttc
      121 gcccagcgac ggctcacctt cccttcgatc atccctttaa tcgatccttt cacccagaat
      181 gccatataca tatggatcag gatggcgtgg atcaggatga tacccgcagc cgcgtgaatc
      241 agcaggctgt agcgaacaac ctgcatcggg aagtactgcg caaagtacgg acgccagata
      301 atcaccccgg tcaccagcag cacgaaaatc atgctcatga tcgaccagaa catcatcttc
      361 tgcccggcgt tgtacttacc aacatccgcc actttgtgct cattgccttt taatacttcg
      421 acaatgttca acagccacgg aatatccttc ttatccggga tattgtgatg tacaaaacgc
      481 acaaacataa acatcagcgc gacgaaaatc gcaatgccga agaacgggtg caaaatgcgt
      541 cccatctgcg gcgtaccgaa ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa
      601 atcccggaca gcgccaccag gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca
      661 ataaacttgg tgcgcacaat cattttcgac ttactcatga tgctcctcct cgtcatcgtc
      721 cacttcctta ttcgggccaa taccgatgta gtggaaaatc aacccggcaa aagtggcgat
      781 aaagccagcc gctgccagcg gtttcaacgc gcctttccac aagttcaccg atgtgtcgat
      841 cttcggatct ttcggcagac cgtgatacag ctccggctga tcggcgtgat gcaacacgta
      901 catgacgtgc gtaccaccga ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg
      961 cgctttcagt ttcgctacgc gctgttccgc cagctccagc atctccttct tggtgccgaa
     1021 gtggatagcc ccggtcggac aggttttcac acaagccggt tcctggccga cgctgacgcg
     1081 atcgacgcag agcgtgcatt tatatacccg gttatcctct ttgttgaggc gcggaatatt
     1141 aaacggacac ccggcaatgc agtaaccaca gccgatgcag ttttccgact ggaaatcaac
     1201 aatcccgtta gcgtactgaa tgattgcacc agcagacggg cacgccttca ggcagcccgg
     1261 atcttcacag tgcatacagc catctttacg gatcagccac tccagcttgc cgttctgttc
     1321 ggtttcgcta aagcgcatca ccgtccagga cttggcgctc agatcggcgg ggttatcgta
     1381 aaccccgacg cagtgcccca cttcatcacg gatgtcgttc cactccgaac acgccacctg
     1441 acaggcttta cagccgatac aggtggaaac gtcgataagt ttcgcgactt ctgctttgta
     1501 atcacgcacc tgagaaggcg gcgtgatgga gttagttgcg gaccttttga taatgtcctg
     1561 cgtttccata gccatctgtt cgccccctta cgccttctcg atgtttacta agaacgcttt
     1621 atattccggc gtttgcgagt ttgcatcacc gacattcggc gtcagagtgt tagcgatata
     1681 acctttacgc gcgacaccct caaagcccca gtggattgga atacccaccg tttcaacctg
     1741 ctgaccgttt acattcagcg gtttcagacg gcgcgttacc acagccaccg cgcggataaa
     1801 gccacgcttg ctggagacag tgacacgatc gccattggca atgcctttcg ccgccgccag
     1861 cgtttcgctg atttccacaa actgttccgg ctgagcaatt gcgttgagca atgcgtgctt
     1921 ggtccaggtg tggaagtgct ccgtcagacg ataggtcgta cccacatacg ggaactgctc
     1981 ttttttaccc atccgcagtg cgtcttgttc atacagacga acaaccgggt tagacaccac
     2041 gttcggatgc aacgggttag tgcccagcgg cgtttcaatt ggctcatagt gttccgggaa
     2101 cggaccttcc gccattttgt tgatggcaaa caggcgtccc atcccttccg gctgcatgat
     2161 aaacggcccg gttggcgtac ccggtgcggc attgccgaag tccggaatat cgttacccgt
     2221 ccacttgcta ccgttccact ggatcagcat ccgtttcgga tcccacggtt taccgttgat
     2281 atccgccgaa gcacggttgt agagtacgcg acggttgagc ggccacgccc aggcccatcc
     2341 cagcgtattc cccagaccgg acgggtcgga gttatcgcgg ttagccatct ggttgccctg
     2401 ctcggtccag ctaccggtgt agatccagca agaagatgcg gttgtaccgt catcacgcag
     2461 atgcgcaaag ctactcagca actgaccttt cttcgcaatc agcacgccat tagcgtcata
     2521 gagatcttcc agcgcatagc cgttgttctc tttcgccact tcgtcagact gcggttcgtg
     2581 cggctgcttg tagttccagc tcatcttcat cagcggttct acgcctttac caccctcggc
     2641 ctggtacagc tcgcgcagat gatggtagat acccgccaga atttcgccgt cgttacgtgc
     2701 ttcgcccggc gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg agttagcgat
     2761 agaaccatct tcttcagcaa agcaggtcga aggcagacgg aatacttcag tctgaataga
     2821 tgccggatcg acatcgttcg actcgccgtg gttctgccag aaggtagagg tttcagtcac
     2881 cagcggatcg ataaccacca tgtacttcag tttgctcagg cagctcacca ctttgttttt
     2941 gtccgggaag gacgcaaccg ggttaaagcc ctggcagaaa taaccggtga ctttaccttc
     3001 atccatcatg ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc
     3061 gtagccccag ttgttctctt tctgcgcggc atcgccgtag aaagatttca tcaggctaac
     3121 gaaaaacttc ggatagttgc tccagtagtt cacctgatca gccagcgtcg ctttcggcgt
     3181 gttcgcttcc agatacgact gcaaatcaac ctgtttttct gacggcagcg tcagataacc
     3241 tggcaggctg gtagagagca ggcctaagtc agtcaggccc tgaatgttgg agtgaccacg
     3301 caatgcgttc acgccgccac cggccatacc catgttaccg agcagcaact ggatcatcgc
     3361 catcgtacgg atgttctgcg cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa
     3421 ggtggttgtg cgatccggtg cgctggtgga ggccagcact tcacacactt tcaggaagtc
     3481 ggcttttggc gtaccacaga tgttttcaac aacgtccggc gtgtagcggg aaacgtgcgc
     3541 tttcagcagg ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc
     3601 gttttcatcg aactgatagt tccaggacga tttatcgtat tgccgttttt cagcgtcgta
     3661 gccgctgaac agaccgtctt cgaaagcaaa atcatcacgc accagcaggc tggcattggt
     3721 gtaatgctta acgtattcgg cattgatttt gttgttttcg atcaggtagc gcaaaacgcc
     3781 agacaggaac gtaatgtccg taccggaacg aataggtgcg taaatatccg ctacagaagc
     3841 ggtacgcgta aaacggggat cgacaacgat caaggttgca tcgttgttgt ttttcgcttc
     3901 catcgcccag cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca tcaccattac
     3961 gacgttagcg tttttgatat ccacccagtg gttggtcatc gcaccgcgac caaatgttgg
     4021 agcaagactt gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta ctgccagcat
     4081 cccgagggag cgggcaaatt tttgagtcag catcccggtt tcgttgctgg caccggaggc
     4141 acacagcata ccggtagaaa gccaacggtt taccgttacg ccctgctcgt tcttttcaat
     4201 aaagttagcg tcacggtcag ctttcatcag cttcgcaata cgggagaatg cttcttccca
     4261 gctaatgcgc tgccatttgt cagaacctgg cgcacgatat tccgggtagc gcagacggtt
     4321 ttcgctgttg acgtaatcca gcaaaccggc ccctttcggg cacagcgcac cacggcttac
     4381 cggatgatcc gggtcacctt caatgtgata aatcgcttct ctggcgtttt tcgcgccatc
     4441 acccaggcta tacatcaata gcccgcaacc tacggaacag tatgtgcagg tgttacggat
     4501 ctctttcgcg cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa
     4561 gcccaatgcc gctaccgttg ttccagccat accgcccgcg cagattttaa aaaattgtct
     4621 gcgactgacg tccattgctt tcctcttttt tcaggggtat tactgcgcga ggaaactaac
     4681 agtaaaattc tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa
     4741 tccgcctgcg caaattgacc tacctcaata gcggtagaaa aacgcaccac tgcctgacag
     4801 gccagttaaa aaaatgctat aaaattcagc ttaattttta acggcaagag agacaaaaca
     4861 gcgagcatga cacgacaaaa agcaacgctc atagggctaa tagcgatcgt cctgtggagc
     4921 acgatggtag gattgattcg cggtgtcagt gaggggctcg gcccggtcgg cggcgcagct
     4981 gctatctatt cattaagcgg gctgctgtta atcttcacgg ttggatttcc gcgtattcgg
     5041 cagatcccga aaggctattt actcgccggg agtctgttat tcgtcagcta tgaaatctgt
     5101 ctggcgcttt ccttagggta tgcggcgacc cgtcatcagg cgattgaagt ggggatggtg
     5161 aactatctat ggcccagcct gacaattctc tttgccattc tgtttaatgg tcagaaaacc
     5221 aactggctga ttgtaccagg attattatta gccctggtcg gcgtctgctg ggtgttaggt
     5281 ggtgacaatg ggttacatta tgatgaaatc atcaataata tcaccaccag cccattgagt
     5341 tatttcctgt cgtttattgg tgcgtttatc tgggcagcct actgcacagt aacgaataaa
     5401 tacgcacgcg gatttaacgg aattaccgtt tttgtcctgc taacgggagc aagtctgtgg
     5461 gtttactatt ttctcacgcc acaaccagaa atggtattta gcacgccagt catgattaaa
     5521 ctcatctctg cggcatttac cttaggattt gcttatgctg catggaacgt cggtatatta
     5581 catggcaacg tcaccattat ggcggtaggt tcgtatttta cgcccgtcct ttcctcagcg
     5641 cttgcagccg tgctgctcag cgccccgctg tcgttctcgt tctggcaagg cgcgctaatg
     5701 gtctgcggcg gttccctgct ctgctggctg gcgacacgtc gtggctaaaa taattgtcgg
     5761 gtctacggcc cggcagttaa tcagataaat tctgacaata ttaaaaaata aatttcaaat
     5821 accagaaagc cattttagtt acatttagta acacatatct gtctgtttaa tccacattct
     5881 gcccccgcag ttacgctttc ttatctattc ttctaatcag cacttcacct gattcatcct
     5941 taattctaat aactggctat tgcgccttac ctcctgtttt gtcttttttt cattaccaat
     6001 gtgtgcatgt gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac
     6061 gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaagcat attgcacgaa
     6121 gaaacctctg gatatcagtc agttgcctac ttcttgcctt ctgtgtctgg atgctattta
     6181 gcgcagttac tgttaatctc aataaaatcg gttttaattt cactaccgat caactctttt
     6241 tattaaccgc attaccctcc gtttctggcg cattattgcg tgtcccctac tcctttatgg
     6301 tgcctatatt cggtggacgc cgatggacgg tttttagtac tgcaatcctg attattcctt
     6361 gcgtctggct cggaattgcc gtgcagaatc cgaatactcc ttttgggata tttatcgtta
     6421 tcgctttgct atgcggtttt gcaggtgcga actttgcttc gagcatgggc aatatcagtt
     6481 tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact
     6541 taggtgtaag tgtgatgcag ctggttgcac cgctggtcat ttttgtcccc gtatttgcct
     6601 ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg
     6661 catggatttg ggtgccatta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg
     6721 atatcgccag ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc
     6781 tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg
     6841 gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct
     6901 ttggcccatt tatcggtgcc atcgcgcgat cggttggtgg tgctatttcc gataaattcg
     6961 gcggcgtgcg ggtgacgttg atcaacttca tttttatggc gattttcagc gccctgctgt
     7021 tccttacctt accgggcaca ggctccggta atttcatcgc attttacgcc gtatttatgg
     7081 ggctgtttct gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct
     7141 ttcgccagat aacaatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaaa
     7201 gagaagccat caccgaaacg gcagccgctc tgggctttat ctcagccatt ggcgcagtgg
     7261 gcggcttttt tattccgcag gcgtttggca tgtcgctcaa tatgaccggc tctccggtgg
     7321 gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctactgacc tggctggttt
     7381 atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgacacgtc ttatcaggct
     7441 acaaccgtgt cgcatccgac aaggcatcag atcccaattc attcctggag caggagttat
     7501 gtcatgagta aacttttgga tcgctttcgc tacttcaaac aaaagggcga aacctttgcc
     7561 gatggtcacg gacaggtgat gcatagcaac cgcgactggg aggacagcta tcgccagcgt
     7621 tggcagttcg acaaaatcgt gcgttccacc cacggtgtta actgtacagg ctcctgtagc
     7681 tggaaaatct acgttaaaaa tggtctggtg acctgggaaa tccaacagac cgactacccg
     7741 cgcactcgcc ctgacctgcc aaatcatgaa cctcgcggct gcccgcgtgg cgcaagttac
     7801 tcctggtatc tctacagcgc taaccgcctg aaataccctc tcattcgtaa acgactgatt
     7861 gaactgtggc gcgaagccct caagcaacac agcgatccgg tactggcgtg ggcatcgatt
     7921 atgaacgatc cgcaaaagtg cctgagctac aaacaagtac gtggacgcgg cggctttatc
     7981 cgctctaact ggcaggagct aaatcagctg attgctgccg ctaacgtctg gaccatcaaa
     8041 acctacggcc cggatcgcgt tgccggtttc tcgccgatcc cggcgatgtc gatggtttct
     8101 tacgccgccg gaacacgtta tctttcacta ttaggtggca cctgtttaag cttctacgac
     8161 tggtattgcg acctgccgcc cgcctcgccg atgacctggg gcgagcaaac cgacgtaccg
     8221 gaatctgccg actggtataa ctccagttac atcatcgcct gggggtctaa cgtaccgcag
     8281 acacgtacgc cggacgccca tttctttacc gaagtacgct acaaaggcac taaaaccatc
     8341 gccattaccc ctgactactc tgaagtggcc aaattgtgcg accagtggct ggcaccgaaa
     8401 caaggcactg atagtgccct ggcaatggca atgggccatg tcattttaaa agagtttcat
     8461 ctcgataatc ccagcgatta ttttattaac tactgccgcc gctacagcga catgccgatg
     8521 ctggtaatgc tggaacctcg cgacgacggc agctacgttc ccgggcgcat ggtccgcgca
     8581 tctgacctgg tggatggact gggcgaaagc aataatccgc agtggaaaac cgtggcagtt
     8641 aataccgcag gtgaactggt agtgccgaac ggttcgattg gttttcgctg gggagaaaaa
     8701 ggcaaatgga atctggaatc cattgccgcc ggtacggaaa ccgaattgtt gttaactctg
     8761 ctcggtcaac atgatgccgt tgcaggcgtg gccttcccct actttggcgg cattgaaaat
     8821 ccgcattttc gcagcgtaaa acacaatccg gtcctggtgc gccaattgcc cgttaaaaac
     8881 ctgacattag ccgatggcaa cacctgtccg gtcgtcagcg tttatgattt ggtactggcg
     8941 aattatggcc tcgatcgcgg gctggaagat gaaaacagcg cgaaagatta cgctgaaatc
     9001 aaaccgtaca ccccagcctg gggtgagcaa attaccggcg tgccgcgcca gtatattgaa
     9061 accatcgccc gtgaatttgc cgataccgcc cataaaacgc atgggcgctc gatgattatc
     9121 ctcggtgctg gcgtcaacca ttggtatcac atggacatga actaccgtgg gatgatcaac
     9181 atgctgatct tctgcggttg tgtcgggcaa agcggtggcg gctgggcaca ctatgtcggt
     9241 caggaaaaac tgcgcccgca aaccggctgg ttgccgctgg cctttgcgct cgactggaac
     9301 cgaccaccgc gccagatgaa cagcacctcg tttttctaca atcattccag ccaatggcgc
     9361 tatgaaaaag tctttgctca ggagttactt tcaccgctcg ccgatgccag taagtacagc
     9421 ggtcatctga ttgatttcaa cgttcgtgcc gaacgtatgg gctggctacc ttctgcgccg
     9481 cagctggggc gtaacccgct cgggattaaa gctgaagccg acaaggccgg attatccccc
     9541 acagaattta ccgcccaggc gctgaaatcg ggcgatttac gcatggcctg cgaacaacca
     9601 gatagcggca gcaatcatcc gcgtaatttg tttgtctggc gttctaacct gcttggctcc
     9661 tccggcaaag gccacgagta tatgcagaag tatctgctgg gtacggaaag tggcattcag
     9721 ggggaagaac ttggaccgac ggagggaatc caaccggaag aagttgagtg gcaaactgca
     9781 gcgattgaag gcaagctcga cctgctggtg acgcttgact tccgcatgtc cagcacctgc
     9841 ctgttctccg atatcgttct gcccaccgcc acctggtacg aaaaagacga tatgaatacc
     9901 tcggatatgc atccgtttat tcatccgctt tctgcggcgg tcgatccggc gtgggaatca
     9961 cgcagcgact gggaaatcta caaaggtatc gctaaagcct tttcgcaagt gtgcgtgggg
    10021 catctcggca aagaaaccga catggtatta caaccactgc tgcatgactc cccggcagag
    10081 ctttcacagc cgtgtgaagt gctcgactgg cgcaaaggtg aatgcgatct gattccaggc
    10141 aaaaccgcgc cgaatattgt ggcggtggag cgcgactacc ctgctaccta tgaacgcttt
    10201 acctcactcg ggccattgat ggacaaactt ggcaacggcg gtaaagggat ttcgtggaat
    10261 acccaggatg aaatcgattt cctcggtaaa ctcaattaca ccaagcgtga tggcccggca
    10321 caggggcgtc cgctgattga taccgcgatt gacgcatcag aagtgattct ggcgctggca
    10381 ccggaaacca acggccatgt tgcagtcaaa gcgtggcaag cgctgggcga gatcaccgga
    10441 cgcgaacata cccatctggc gcagcacaaa gaggacgaga agattcgctt tcgcgatatt
    10501 caggcgcagc cgcgtaaaat tatctccagc cccacatggt ctggtctgga aagcgatcac
    10561 gtttcctata acgcgggata caccaacgtt catgagttaa ttccgtggcg cacgctgtcg
    10621 ggacgccagc aactctatca ggatcatccg tggatgcgtg cttttggtga aagtctggtg
    10681 gcttatcgcc cgcctatcga cacccgcagt gtcagtgaaa tgcgccagat accgccaaac
    10741 ggcttcccgg agaaagccct taacttcctg acaccgcacc agaaatgggg cattcactca
    10801 acctacagtg aaaacctgct aatgctgacg ctctctcgcg gtggaccgat tgtctggatc
    10861 agcgaaaccg atgcccgtga actgaccatt gtcgataacg actgggtgga agtgtttaac
    10921 gccaatggcg cgctgacggc ccgcgcggtg gtcagccagc gtgtaccacc gggcatgacc
    10981 atgatgtatc acgctcagga acgcattatg aatattccag gttcggaagt aaccggcatg
    11041 cgcggcggca ttcataactc ggtcacccgc gtttgtccga aaccaacgca tatgattggc
    11101 ggttacgcgc agctggcctg gggctttaat tactacggca ccgtcggctc gaaccgtgac
    11161 gaattcatca tgatccgcaa gatgaagaac gttaactggc tggatgatga aggtcgcgat
    11221 caggtacagg aggcgaaaaa atgaaaatac gttcgcaagt cggcatggtg cttaacctcg
    11281 ataaatgtat cggctgccat acctgttcgg tgacctgtaa aaacgtctgg accggacgcg
    11341 aaggcatgga gtacgcatgg tttaacaacg tcgaaaccaa accgggcatt ggttatccga
    11401 aaaactggga agatcaggaa gaatggcaag gcggctgggt gcgtgatgtg aatggcaaga
    11461 tccgcccgcg tctgggcagc aagatgggcg taataaccaa aatcttcgcc aacccggtgg
    11521 tgccgcagat tgatgattac tacgaacctt tcaccttcga ctacgaacat ttgcatagcg
    11581 caccggaagg caaacatatc cctactgctc gcccgcgttc actgattgac ggcaaacgga
    11641 tggacaaagt gatctggggg ccaaactggg aagaactgct gggcggcgaa tttgaaaaac
    11701 gcgctcgcga ccgcaacttc gaggccatgc aaaaggagat gtacgggcag tttgaaaaca
    11761 ccttcatgat gtacctgccg cgcctgtgcg aacactgcct caatcccagc tgcgtggcga
    11821 cctgcccaag cggtgctatc tacaaacgtg aagaagacgg cattgtgctg attgatcagg
    11881 ataaatgccg tggctggcgt ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca
    11941 actggaaaag cggcaagtca gaaaaatgta tcttctgtta cccacgaatt gagtcaggcc
    12001 aacccaccgt gtgctcagaa acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt
    12061 acgacgccga ccgcatcgag gaagcggcga gcaccgagcg cgaagttgac ctctatgaac
    12121 gtcagtgcga agtgttcctc gatccgcacg atccctcagt gatcgaagaa gccctgaaac
    12181 aaggtattcc acaaaacgtg attgaagctg cccagcgttc gccagtctac aaaatggcga
    12241 tggactggaa actggcgtta ccgctgcacc ctgaataccg caccctgccg atggtctggt
    12301 acgttcctcc gctgtcaccg attcagtctt acgctgatgc gggcggtttg ccgaaaagcg
    12361 aaggcgtgct gcctgctatc gaaagcctgc gtattccggt gcaatatctc gccaatatgt
    12421 tgagtgctgg cgataccggt ccggtactgc gggcgctgaa acggatgatg gcgatgcgcc
    12481 actatatgcg ttcacaaacc gtggaaggcg ttaccgatac tcgtgccatc gacgaagtag
    12541 gcctgaacgt cgcccaggtc gaagagatgt atcgctacct cgccattgcc aactatgaag
    12601 accgttttgt cattccaacc agtcatcggg aaatggcggg cgatgccttt gctgaacgca
    12661 acggctgcgg ttttaccttt ggcgacggtt gccacggctc ggacagcaaa ttcaacctgt
    12721 tcaacagtag ccgtatcgat gccatcaaca tcaccgaagt gcgcgacaaa gcggagggcg
    12781 aataatgcag atcctcaaag tgatcggcct gttgatggag tatccggacg agctgttgtg
    12841 ggaatgtaag gaggacgcgc tggcgttgat ccgccgcgac gcaccgatgc tcaccgattt
    12901 tgcactcgac ctgctcaacg cgccgctgct ggataaacag gccgaatggt gtgaagtgtt
    12961 tgaccgcggg cgcaccacgt cgctgctgct gtttgaacat gttcatgccg agtcccgcga
    13021 tcgcggccag gcaatggtgg acctgctggc ggagtatgaa aaggtcggtc tgcaactgaa
    13081 ttgtagggaa ctgcccgatt atctaccgct gtatctggag tatttaagca tacttccgga
    13141 cgatcaggcg aaagaaggat tactcaacgt tgcgccgatc ctcgccctgc ttggcggacg
    13201 cttaaaacaa cgcgaggcac cgtggtacgc gttatttgac gccctgttgc aactggcagg
    13261 aagccctcta tcaagtgaca gtgtcacaaa acagattcgc agtgaagagc gtgacgacac
    13321 ccgccaggcg ctggatgcgg tgtgggaaga agaacaggtg aagtttattg aagataacgc
    13381 cacggcgtgt gacagctcgc cgcttaatca atatcagcga cgctttagcc aggatgtcgc
    13441 gccgcagtat gtcgacatca gtgcgggagg tgggaaatga ttcagtatct gaacgtcttt
    13501 ttttacgata tctacccgta cttgtgtggc acggttttta tccttggaag ctggctgcgc
    13561 tacgattacg ggcagtatac ctggcgtgcc gcctccagcc agatgctgga taaacgcggg
    13621 atggtgctat ggtcgaactt gttccatatc ggcattctgg ggatcttctt cggtcatctg
    13681 ttcggcatgt taacgccgca ctggatgtac gcgtggtttc tgccggtggc ggtaaaacag
    13741 cagatggcga tgatcctcgg cggaatatgc ggggtattga cgctgattgg cggtgcgggg
    13801 ctactggtgc gtcgtttaac aaatccgcgt atcagggcga cgtcttctgg tgcggatatc
    13861 ctgatcctcg ccattttgtt aattcagtgc attcttgggt taaccaccat tcctttttca
    13921 gcacaacatc cggacggcag tgaaatgatg aagctggtgg gctgggcgca aagcattgtc
    13981 actttccagg gcggtgcatc tacgcatctt gatggtgtcg cctttatatt ccgcgttcat
    14041 ctggtgcttg gaatgaccat cttcctgctc ttcccgttca cccgactggt gcatgtctgg
    14101 agcgcgccgt ttgagtactt tactcgtcgg tatcagattg tgcgttcgcg gcgttaattc
    14161 tcacgcgggc cggacagccc ttcgctctcc ggccacttgc aggctatgat agttctccag
    14221 ccacaggaga acattaatga aaccgcaggt ttaccacgtc gatgctttta cctctcaacc
    14281 gtttcgcggc aattctgccg gtgttgtttt ccccgccgat aatctcagtg aagcgcaaat
    14341 gcagcttatc gcccgcgagt taggtcattc ggaaactgct tttctgctgc acagcgacga
    14401 cagcgatgtg cgcatccgct actttacgcc aacggttgaa gttccgattt gtggtcacgc
    14461 gacagtagct gcgcactatg tacgtgcaaa ggtgttaggt ttaggaaatt gcacggtctg
    14521 gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa aagcacaacg atgattatcg
    14581 tatttcgctg gaacaaggta cgccgggctt tgagtcaccg ctggaaggtg aaacacgtgc
    14641 ggcgattatc aacgcgcttc atctcactga agacgatatt ctgccaggct tgccaattca
    14701 ggtggcaacc acaggacact caaaagtgat gatcccactg aaaccggaag tggatatcga
    14761 cgccctttcg cctgacctta atgcgctgac cgctatcagc aaacagattg gctgcaatgg
    14821 tttcttcccg ttccagatcc gcccagacaa aaacgaaacc gacggtcgta tgttctcgcc
    14881 tgccattggt attgtggaag atccggtgac tggcaatgcc aatggcccga tgggtgcatg
    14941 gttagtacat cacaacgtat tgccccacga tggcaacgtg ttgcgtgtta aaggccatca
    15001 ggggcgcgca ttagggcgtg acggcgtgat tgaagtgacg gtgacaattc gtgataacca
    15061 accggagaaa gtcaccattt ctggcgtggc ggtgattttg tttcatgccg aatgggcaat
    15121 tgaactctga aaccgttgcc tgatgcgctt cgcttatcag gtctacaacc gggctaatgt
    15181 aggccggata aggcgtttac gccacatccg gcaacctgac ataaattatt ttcccgcctc
    15241 cgggtgcgta tcaaacgccg ccatcaccag cgctaactca tccacagtaa agccatgttt
    15301 cgcatcatct acgcccagac caaactgttc ctgcatcacg gcatataacg acgccacatc
    15361 tggtagattt cgctgctcaa ctgcgtgccc attttcataa tgggtaaaat gaaaattggt
    15421 cagcgttagc ttgccaccgt ccggcaaatg gcgacacatc agcaaatgat ggcgaaaatg
    15481 cgactgcggc cagtgcgccg accagaaatt gcccatcaca taatcgcttt gttgctgctc
    15541 gcagagatca aaacggtaca tcgactgcca gtgctgatga tgattaaact gcaacaccca
    15601 atcatcaccc tcttgcaaca accgatactc tccgtgtggc gtggtttgca tgatatccgg
    15661 cattaaacga atcggcgcgg ttagcgtctg cccaccgaaa ccgacatcag caatccattt
    15721 ttccccttcc agttccacca acagcaaacg atgggtgcgc ggcggtaatg ctggcggatt
    15781 tgataacact acgcgcccta acaagctgcg aacgttaaac cccagctcgc gtaacacccg
    15841 ctcaaacacg ccattctgct caaaacagta accaccacga cgggctatca ccagtttctc
    15901 ttccagcgat tgatcatcaa gctgtatttc cctcggcagc aaaacgtcga ggttttcaaa
    15961 cggaatggtg caattgtgtt tcaggtgcaa tgcacgaagc gtatcaatat tggccgcagc
    16021 agctcccgac cagttaatac gggcaaaata atgattcaga atgggcgtca tcaggcgttt
    16081 cccttagtgg ttttctcaac ttataacctg gaatagacgc cgcaccggct attttgtgcg
    16141 atcgcggaca tgcatcacgt tcgcaaatcg ccaatcccgt taatttcgtt acttattgcg
    16201 ttacgagcta cgcttttaaa acacccttca tagcgaggcg atatcgtgag ccaattcatt
    16261 cccgtcgaat tacaccatgc cagccgtctg ttaaatcatg gcccaactgt cttgatcacc
    16321 agtttcgatg agcaatccca gcggcgtaac attatggctg cagcctggtc aatgccggtg
    16381 gagtttgaac ctccacgcgt ggcgattgtg gtagataagt cgacatggac cagggagttg
    16441 attgagcgta acggtaaatt tggcatcgtt atcccaggcg ttgcagcgac taactggacg
    16501 tgggcagtgg gaagtgtatc tgggcgtgaa gaggataaat tttattgcta tggcattccg
    16561 gtggtgaaag gtccggtgct tggtttgcca cttgttgaag agaaatgtct ggcgtggatg
    16621 gagtgccgat tgctaccagc gacttccgcg caagagaaat acgacacgct gtttggcgaa
    16681 gtggtatcag cagcggcaga cgcacgggta tttgtcgaag gccgctggca gtttgatgat
    16741 gataagctca atacgttgca tcatttaggc gctggcacgt ttgttaccag cggcaagcga
    16801 gtcaccgcgg gctaaagaat catatgcagc aaccaagttg cttaagcaat gtttcaatat
    16861 gagttaaaag catattttat ctcacgattg catattttta gtgaagatta atttatcctc
    16921 accaacatca tagtaattgc gtattatagc ttcattgata aagccctgtt atgtataaaa
    16981 tgcgcgggcg gcactaaaat cgacatagct ggaggtctct actaaaagta gtcttgcctg
    17041 tttaattgtg agctcctttt ctatttcgtt aatcagcaga tgtcccactc cctggcgctg
    17101 atgggaagga ctgatccagt gcattaataa attccagaca tcatttgtca tcacttcagg
    17161 agcgcaatag gcaatccctg ccagtcctaa gtgttctgca ctaaaccata aatcggcaga
    17221 ttgacctgag aaataatgtg ttagtgtttt ttgtacatga tgaaggccat cttgattaaa
    17281 ttgtccactg gcaagcagca tatccagtaa tggttcaaca tctgaaggtt tgactggtcg
    17341 tataatcatg gaattcctta tctattgatg aatgtttgaa aattgtttgc gtggaacatg
    17401 gttaatctga gccaatattt ttgagaaaga atagatgggt atttatataa gcaaagaagc
    17461 catctcttta aatatcaatt aaagtataac attaacacca gacttcattg cgacaggaca
    17521 cgacaaagca cttttgcggt tttttcaggg tgcaattaca aaaattgaat cccttatgga
    17581 ctaatgtgag aaaattccgg ggcatgacta ttttgatata gtggacaatc aagcgtctcg
    17641 aattaacaat gacttttcag atgctaaacc atgcaattgg aagtatgtcg gatataggac
    17701 aacattagga aatcggacag aaaattcatc ataataaaat gacaacttat ctctttctct
    17761 cactttctga gaaatttcac aaaagtataa actgagccaa tatgccttta tatataaaaa
    17821 atactcatgc ttatttttat cggaaaaagc caagattttg tcaaggccat caaaaatatc
    17881 tttatcatga tctatatgta ctcttgatga tttcacaata cagggaaaga ttacgaaagc
    17941 ccgctccccg caaggactga cgccagatag tttttgtcca tggctgcttt tcgcatctta
    18001 cgtcttaacc ctgccttgaa taccttatca ttcgttaaaa tattaatagc gatgt
//
LOCUS       DAZGHF010000096        17934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGHF010000096 DAZGHF010000000
VERSION     DAZGHF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.096"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            33..599
                     /locus_tag="ACHK3A_003397"
     CDS             33..599
                     /locus_tag="ACHK3A_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8741441.1"
                     /translation="MSKFVKAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCS
                     IVPDDHKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMTTTFTGNPYSGN
                     SKNYSLSNMDNGTEIPNVSLVIGDQRGTGYDLGAGIDQDIAMDSSTGKGKQKQTLNFK
                     AWLVGETAAVTPTPAPFETLTTFQITYL"
     gene            685..3324
                     /locus_tag="ACHK3A_003398"
     CDS             685..3324
                     /locus_tag="ACHK3A_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO8741442.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNVLADDTIQFDARFLELKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNHPLPDEYDIYWYVAENDPNKSYACLPPELI
                     AQFGFKDDFAKSLQWGHDGQCLKTDQIGGMEIKGDLSQSALLVSVPQAYLEYTDDDWD
                     PPSRWDEGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNMRGYAPDISGVARTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALFGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDTDRARTGNDKEMY
                     TVTYNQNFRDARVSVYLNYSHHTYWDREDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSSQASLYHRIDDASHYTLSAGTS
                     ENHTSLDGYYSHDSTLAKVDLSANYHEGQYTSAGVSLQGGVTLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATQ
                     SVVQATLTEGAIGYRKFAVISGEKAMVVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSAPVITHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            3344..4093
                     /locus_tag="ACHK3A_003399"
     CDS             3344..4093
                     /locus_tag="ACHK3A_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8741443.1"
                     /translation="MTKHLSVKTLATVLSLLAATVSFSALASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKQPYLAQSWIEDEKGVKISSPLAVLPPVQRIDAMMNGQVKIQGLPDIH
                     KLPADRESIFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALEKASMQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPADGFSPIVMSPKTSAPLKVKMASVPVL
                     TYVNDYGARMPLFFTCSGNSCQVDEELSRKG"
     gene            4110..4616
                     /locus_tag="ACHK3A_003400"
     CDS             4110..4616
                     /locus_tag="ACHK3A_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8741444.1"
                     /translation="MKRSCSVWAGTWLLMCCIGSDFAVAGSKSVNLSLRVLVDAPPPC
                     TVNGATVEFGNVFINKIDGVGYKQPIDYSLVCNNLAMDDLRLQMRATTVVINGETVIS
                     TGISGFGIRVQKSSDHSTLDLTSGSWLPFNFSSGVPALEAVPVKQSGTTLSAAEFNAS
                     ATIVVDYQ"
     gene            4613..5083
                     /locus_tag="ACHK3A_003401"
     CDS             4613..5083
                     /locus_tag="ACHK3A_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8741445.1"
                     /translation="MKRISLLMLWGFSSMALSNVSFHGYLVQPPNCTISNGKTIEITF
                     QDVFVDDINGSNYEQTVPYSITCDTAVRDPSMEMKLSWSGTQSDFDDAAVSTDISGLA
                     IRLKQAGQSFKLNTALVVNETALPVLTAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            5080..5604
                     /locus_tag="ACHK3A_003402"
     CDS             5080..5604
                     /locus_tag="ACHK3A_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8741446.1"
                     /translation="MKKKRALVFICSLMLLYSVTAPAVDNLHFTGNLLGKSCTPVING
                     SLLAEVHFPTIAASDLMNLGQSERVPLVFQLKDCKSSTLFSVRVTLTGTEDSELPGFL
                     AFDASSSASGAGIGIETAAGAAVPVNSTTGVTFPLNQGNNSLNFNAWLQAKGGRDVTS
                     GDFSATATATFEYF"
     gene            5606..6448
                     /locus_tag="ACHK3A_003403"
     CDS             5606..6448
                     /locus_tag="ACHK3A_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO8741447.1"
                     /translation="MKKWTMIFASLVLLVMSASVPKSYAGDKLTSASFDSVKIYYGKG
                     KMSIHNTGGLYVTVLTPNEAAYGVYNDAIRKNEQASVISWTGSGPAPVLILTDYGNAD
                     SSNCPGINTATFQCAYETFKITVQSDSYGCPWLASFYTITDQPGFGRYTGPTNHATVC
                     PTIPVASYDISWSENYVSHNKALQLQSNGSTITTTLSTYLMEDGKLCDGSVFDSRGAY
                     CRVVSELLTFTSYGCDKSTVTVSPTRHPVTDKQLHDIVVNVNTSSRQPIDSTCRFQYV
                     LNEL"
     gene            complement(6564..7115)
                     /gene="smrB"
                     /locus_tag="ACHK3A_003404"
     CDS             complement(6564..7115)
                     /gene="smrB"
                     /locus_tag="ACHK3A_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO8741448.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7281..8213
                     /gene="prmB"
                     /locus_tag="ACHK3A_003405"
     CDS             7281..8213
                     /gene="prmB"
                     /locus_tag="ACHK3A_003405"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO8741449.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            8248..9333
                     /gene="aroC"
                     /locus_tag="ACHK3A_003406"
     CDS             8248..9333
                     /gene="aroC"
                     /locus_tag="ACHK3A_003406"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO8741450.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9336..10160
                     /gene="mepA"
                     /locus_tag="ACHK3A_003407"
     CDS             9336..10160
                     /gene="mepA"
                     /locus_tag="ACHK3A_003407"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO8741451.1"
                     /translation="MNKTAIVLLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10160..10969
                     /locus_tag="ACHK3A_003408"
     CDS             10160..10969
                     /locus_tag="ACHK3A_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO8741452.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            10969..11517
                     /gene="epmC"
                     /locus_tag="ACHK3A_003409"
     CDS             10969..11517
                     /gene="epmC"
                     /locus_tag="ACHK3A_003409"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO8741453.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11551..11829
                     /locus_tag="ACHK3A_003410"
     CDS             11551..11829
                     /locus_tag="ACHK3A_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO8741454.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQKASQQ"
     gene            complement(11885..13891)
                     /gene="mnmC"
                     /locus_tag="ACHK3A_003411"
     CDS             complement(11885..13891)
                     /gene="mnmC"
                     /locus_tag="ACHK3A_003411"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO8741455.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHLHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGHVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGSTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RLYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVGANEVEGVTGVV
                     TNCGGITYPQGGWLCPAELTCNVLKLAQQQGLQIRYQHQLKDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSEKEARCGV
                     RCATRDHLPMVGNVPDYDVTLVEYASLAEQKDEAVSAPIYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSNEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            14050..15270
                     /gene="fabB"
                     /locus_tag="ACHK3A_003412"
     CDS             14050..15270
                     /gene="fabB"
                     /locus_tag="ACHK3A_003412"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO8741456.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15535..16713
                     /locus_tag="ACHK3A_003413"
     CDS             15535..16713
                     /locus_tag="ACHK3A_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8741457.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16710..17705)
                     /gene="flk"
                     /locus_tag="ACHK3A_003414"
     CDS             complement(16710..17705)
                     /gene="flk"
                     /locus_tag="ACHK3A_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO8741458.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
BASE COUNT         4285 a         4497 c         4752 g         4400 t
ORIGIN      
        1 tattctaatt atttatgatt taaggacaaa taatgagtaa gtttgtaaaa gcagcagttg
       61 ccacggcaat gctcatgggg gcgctgactt cagcatcagt cgttgccgca ggtaacaacg
      121 gtacagtgag attttacggc acaattgaag actctccgtg ctcgattgtt cctgacgatc
      181 ataaactgga agtggatatg ggcagcattg gtacaggttc tttaaccggt ggcaagacta
      241 ccacacctaa agatttccag attcgtttac aggattgtaa tttcaatact gaaacgacaa
      301 tgaccacgac attcactggt aatccgtact ccggcaactc aaaaaactat tccctgtcta
      361 acatggataa tggaactgaa attccaaatg taagtctggt gattggcgac caacgtggta
      421 ctggttatga cctgggggca ggcatcgatc aggacatcgc gatggacagt tcaacaggaa
      481 aaggcaaaca gaaacaaacg ttgaacttta aagcctggtt agtaggtgaa actgcagcgg
      541 taacaccaac cccagcgccg tttgaaacgc taacgacgtt ccagattaca tatctctaat
      601 tatgctgttc ccccataact gcaatgttat ggggtttata aataaaagat aactaaatta
      661 taataatcat ggatggatac gtagatgcct gaacattctt ttcttcgttt acgggggctt
      721 tcatggtgga ttgctctggc aatttccggg agtcctttta atgtactggc agatgatacc
      781 attcaatttg atgctcgatt tctggagtta aaaggaaata ccaaaattga tctggggcgt
      841 ttttctcaaa aagggtatgt tgaaccaggg aaatacaatt tacgtgttca tgtaaataat
      901 cacccattac ctgatgaata cgatatctat tggtatgtag ctgaaaacga tccgaataaa
      961 tcatatgcct gcttaccacc tgaactgata gcccagtttg gcttcaaaga tgattttgcg
     1021 aagagtttgc aatggggtca tgatggacaa tgtctgaaaa ctgaccaaat aggaggtatg
     1081 gaaattaagg gcgatttaag tcaatctgcg ctcttagttt ctgtgccaca ggcatatctc
     1141 gaatacactg acgatgactg ggacccacct tcgcgttggg atgaggggat tcctgggctt
     1201 attgccgatt acagtatcaa cgcacaaacc cgacatgaaa atggggggga tgacactaac
     1261 gacatcagcg gtaatggtac ggtcggcgtt aacgtggggc cgtggcgctt acgagccgac
     1321 tggcagagcg attaccaaca tacccgcagt aacgatgacg gcgacactga tgatagcggt
     1381 acgcaaaaaa actgggagtg gagccgttac tacgcctggc gcgccttgcc gtcattaaaa
     1441 gccaaacttt cgctgggtga agattatctc aactccgata tttttgatgg cttcagttac
     1501 attggcggca gcatcagtac cgatgatcaa atgttaccgc caaacatgcg cggctacgca
     1561 cccgatattt caggtgttgc acgcactacc gctaaagtga ccgtaaccca aatgggccgt
     1621 gtcatttacg aaacccaagt gcccgcaggt ccattccgca ttcaggatat tggcgattcc
     1681 gtatctggca cgctgcatgt ccgcattgaa gaacaaaacg gtcaggtaca ggaatatgac
     1741 gtcagcacgg cgtccatgcc gtttctgacc cgccccggtc aggtgcgtta caaagtgaca
     1801 atggggcgtc cacaaaactg ggaccatcag gttgcaggca gcttcttctc gggcggtgag
     1861 gcgtcatggg ggattgcgaa tggctggtcg ctctacggcg gggcgttggc cgatgaaaac
     1921 taccagtcgg cagcgttagg cctcggtcgt gacctcgcgc tgttcggagc gctagcgttt
     1981 gatgtcactc actctcgtgt tcaactcgac gataacagcg tgtatggcaa caaaacgctg
     2041 gacggcaact cttatcgcgt cagctatgcc aaagatttcg atgaactcaa cagtcgggtg
     2101 acgtttgccg gatatcgctt ctctgaaaaa aactacatga ccatgagtga gtatctggat
     2161 gcgaacgaca ccgaccgtgc gcgcacgggt aacgacaagg aaatgtacac ggtcacttac
     2221 aaccagaact ttagggatgc gcgcgtttcg gtctatctca attattctca ccatacctat
     2281 tgggatcgcg aggatcagac caactacaac atgatgcttt ctcattattt caatttagga
     2341 agtcttcgca acctgagcgt ttctctcacc ggttatcgct acgaatatga taaaagtgcc
     2401 gacaaaggtg tttatctgtc cttgagtttg ccatggggcg acaacagcac catcagttac
     2461 aacggcaatt atggcagtgg tgccgacagc agccaggcga gtttgtatca ccgcatcgat
     2521 gacgctagcc actatacgtt gagcgccgga accagtgaaa atcacaccag tcttgatggt
     2581 tattacagtc atgacagtac gctggcaaaa gttgacctga gtgcaaacta ccatgagggg
     2641 caatacacat ctgcgggggt ctccttacag ggcggtgtaa cactgactgc tcacggcggg
     2701 gcgttgcatc gtacgcaaaa tatgggaggc acacgtctgc taatcgacgc cgatggcgtt
     2761 gctaatgtgc cggtcgaagg taatggttcc gccgtttata ccaacatgtt cgggaaagcg
     2821 gtggtggcag atgttaatga ttactaccgt aaccaggctt atatcgattt gaacaaacta
     2881 ccggaaaacg ccgaagctac ccaatcggtg gtccaggcaa cgctgacgga aggcgctatt
     2941 ggctaccgta aatttgcggt gatcagcggt gagaaggcga tggtagtgct gcgcttgcag
     3001 gatggcagcc atccgccatt tggggcggaa gtgaaaaacg ataatcaaca acaagttggg
     3061 ctggttgatg acgaaggtaa tgtttatctt gcaggcgtta agccgggtga acatatgacc
     3121 gttttctggg agggagaatc tcactgcgat atcagcctgc cagatccgtt accgaatgac
     3181 ctgttcaatg gcttgttgtt gccatgccag caaaaagggg gaagtgctcc cgtcataacg
     3241 catgatattc aacctgtaat tcaggagcag acacaacagg tgacaccaat ggaaccgccg
     3301 atgtctgttt catcgaacca ataacgtgat taaggaatga tccatgacta aacatctctc
     3361 tgtcaaaaca ttggcaacgg tactgtcgtt actggctgca acggtaagtt tttctgctct
     3421 ggcgtcagta acgcccgatc gtactcgcct gatttttaat gaaagcgata aatccatcag
     3481 cgtcacgctg cgtaataacg acccgaaaca gccttatctg gcacaaagct ggatcgaaga
     3541 tgaaaagggc gtcaagatta gttcgccgct ggcagtattg ccccccgtac agcgcattga
     3601 tgcaatgatg aatggtcagg tgaaaattca gggattgcca gatatccata aattgcctgc
     3661 cgatcgtgaa tcgatatttt atttcaatgt gcgtgaaatt ccgccgaaat cgaacaaagc
     3721 caatacgtta caaattgcgc tgcagacacg catcaaactt ttctggcgac ctaaagccct
     3781 ggaaaaagct agcatgcaaa acccatggca gcataaagtg accttaaccc gtaacggtca
     3841 ggaatttacc gtcaataacc ctactccgta ttacgtcatt ttcagtaatg ccagcgcgca
     3901 aaaaaatggc aatccggcag acggtttctc gccgatagtg atgtcgccaa aaacctccgc
     3961 accgctcaag gtgaagatgg ctagcgtgcc ggtgctgacc tatgtcaacg actatggcgc
     4021 acggatgcca ctctttttca cctgtagcgg caacagttgt caggttgatg aagagctgag
     4081 ccgcaaaggt taatgacggg gaagtaagta tgaaaagatc ttgttcagtc tgggcgggaa
     4141 catggttact gatgtgttgt attggtagcg actttgcagt tgcagggagt aaatcagtaa
     4201 atttgagttt acgagtgctc gttgatgccc ctcctccttg cacggttaat ggtgcgacgg
     4261 ttgagtttgg taatgtattc atcaataaga tcgatggggt aggttataaa caacccattg
     4321 attactcgct tgtttgtaac aacctggcaa tggatgacct gcgtttacag atgcgggcaa
     4381 caacggttgt gatcaacggt gaaacagtta tcagcacagg tatttcaggg tttggtattc
     4441 gggttcagaa aagtagtgat cactcaacac tcgacttaac gtctggttca tggttgccgt
     4501 ttaatttttc ctcgggcgtc cctgcactcg aagcagtgcc ggtgaaacag agtggtacaa
     4561 ccttatcggc agcggaattt aacgccagtg ccacaattgt ggtggattac caatgaaacg
     4621 gatctcactt cttatgctgt ggggctttag ctcgatggca ctctcaaatg tctcttttca
     4681 cggctattta gtgcagccac ctaactgcac tattagcaac ggtaaaacca ttgaaataac
     4741 ctttcaggat gtttttgttg atgacattaa tggcagtaac tatgagcaaa ccgtgccgta
     4801 ttccattacc tgtgatacgg cggttcgcga cccatcaatg gaaatgaaat tgagctggag
     4861 cggtacgcag agcgactttg acgatgccgc ggttagcacc gatatctctg gcctggcgat
     4921 tcggcttaaa caggcagggc agagctttaa gctcaatacg gcactggtgg tgaatgaaac
     4981 ggccttaccg gtgttaaccg cagtaccggt caaaaagagc ggtgtcgatt tgcctgaggc
     5041 agactttgaa gcctgggcca cgttacaggt ggattatcaa tgaagaaaaa gcgtgcgttg
     5101 gtttttatct gtagcctgat gctgttgtac agcgttactg ccccggcggt cgacaacctg
     5161 cactttaccg gtaatttgct cggtaaatcc tgtacgccag tgatcaacgg tagcctgttg
     5221 gcggaggtgc actttcccac cattgccgcc agcgatttaa tgaacctcgg gcagtcagag
     5281 cgagtaccgt tggtatttca attgaaggat tgcaaaagct ccacgctgtt tagcgtcagg
     5341 gtaacgctga cgggaacgga agatagcgaa ctgcccggat ttctggcctt tgacgcctca
     5401 tcttctgcat ccggagctgg gattggtatt gaaacggcgg caggcgcggc ggtgccagtc
     5461 aacagcacaa cgggtgtcac gttcccgcta aaccagggca ataacagtct caattttaat
     5521 gcctggctac aggcgaaggg cgggcgggat gtgacgtccg gtgatttcag cgctacggcg
     5581 acggcaacct ttgagtattt ttaacatgaa aaaatggaca atgatttttg cgagcctggt
     5641 gttgctggtg atgagtgcga gcgttccaaa gagctatgcc ggggataaac tcaccagcgc
     5701 gagtttcgac agtgtgaaga tttattatgg gaaggggaaa atgtctattc ataacaccgg
     5761 aggtttgtat gtcacggtgt taactcccaa cgaggcggcc tatggggtgt acaacgatgc
     5821 cataaggaag aacgagcaag ccagcgttat ttcgtggacg gggtcgggac cagcgccagt
     5881 gttgatctta actgactacg gtaatgctga tagctcgaat tgtcctggga taaatacggc
     5941 gacatttcag tgtgcatatg agacgtttaa gataaccgtt cagtccgaca gttatggttg
     6001 cccgtggctc gcttcttttt atacgatcac agatcaaccc ggattcggga gatatactgg
     6061 gccaacgaac catgccacag tatgtccgac gatccctgtc gcgtcgtatg acatctcgtg
     6121 gagtgagaat tacgtcagcc acaataaggc gttacagttg caatccaatg gctcgacaat
     6181 caccaccacg ctttcgacct acctgatgga agatggcaaa ctctgtgatg gcagcgtttt
     6241 tgatagcaga ggtgcatact gccgcgtggt ctcggaactg ttaaccttta cctcctacgg
     6301 ttgcgataaa tcaacggtaa cggtttctcc cacacggcat ccggtaaccg ataaacagct
     6361 acatgatatc gtggtaaatg tgaacaccag tagccgacaa ccgattgatt ctacgtgccg
     6421 attccagtat gtactgaatg aattgtagtg acgatatttt cacaagtcga cggggccatc
     6481 tggccctgtc gtcatttccg gcgtctgttt tatgatatga tggctaaggc gaactttccc
     6541 tctacagcat tcgatgccag cattcagggc aattcaggcg gcagccactc ttccacttca
     6601 atcaacacca acaaagccgc atcgccacca tactcttttg gtgcctggtg aaatgccatt
     6661 acatgtggat gctgcgccag ccacagcggc gtttgctgct tcaaaatatg cttcccgtga
     6721 ccatgcatca cgcaggcaca aaacacatgt tcacggcggc aggcggcaat caacgccccc
     6781 agttcctgtt tggcttgcaa ctgcgtcaga ccgtgcaaat caagaaacaa ctccggtgaa
     6841 taatcaccgc gccgcagttt cttcgcttca aaatggctga catccgggcg aacatatttc
     6901 accggacctt cggtatttaa taatggctga aactcatcgg aaaaataatg gctggcatcg
     6961 gcctgctcct gaatcaaccg cttgaccggt acttcgctga tttttttacg ctgcggtcgg
     7021 tggacaatcg tgtcctgctt aatcttgcga gtccccgcca tcaactggcg aaacagcgcc
     7081 tggtcctcct cgctaagtgt tgttttcttt ttcatttaac tgtctctatt tctaattttc
     7141 ctacagttta cctgactcgt agcacaatcg atcgttttaa acgtattttt cctcactcac
     7201 cgcgcgtgaa tgctgattta tcgtcatctt catggcaaac tagccgccga aattattgcg
     7261 atcatgccct ggaggaatac gtggataaaa ttttcgttga tgaagcagta aatgagctgc
     7321 aaaccattca ggacatgttg cgctggtcgg tgagccgctt cagcgcggcg aatatctggt
     7381 acggccacgg caccgataac ccgtgggatg aagccgtgca actggtgttg ccttcactct
     7441 acctgccgct ggatattccg gaagatatgc gcaccgcgcg tctgacttcc agcgaaaaac
     7501 accgtattgt tgagcgcgtg atccgccgcg tcaacgaacg cattccggtg gcttacctga
     7561 ccaataaagc ctggttctgt ggccatgaat tttacgtcga tgaacgcgtg ctggtgccgc
     7621 gctcgccgat tggtgaactg atcaacaata aatttgccgg actcatcagc aagcaaccgc
     7681 agcacatttt agatatgtgt accggtagcg gctgcatcgc cattgcctgt gcttatgcct
     7741 tcccggaagc ggaagtcgac gcggtagaca tctctcccga cgcgctggcg gttgctgaac
     7801 agaacatcga agaacacggt ttgatccaca acgtcattcc gattcgttcc gacttgttcc
     7861 gtgatttgcc gaaagtgcag tacgacctga ttgtcaccaa cccgccgtat gtcgatgcgg
     7921 aagatatgtc cgacctgcca aacgaatacc gccacgagcc ggaactcggt ctggcatctg
     7981 gcactgacgg cctgaaactg acgcgtcgca ttcttggtaa cgcggcagat tacctcgctg
     8041 atgatggcgt gctgatttgt gaagtgggca acagcatggt acatcttatg gaacaatatc
     8101 cggatgttcc gttcacctgg ctggagtttg ataacggcgg cgatggtgtg tttatgctca
     8161 ctaaagagca gcttattgcc gcacgagaac atttcgcgat ttataaagat taagtaaaca
     8221 cgcaaacaca acaataacgg agccgtgatg gctggaaaca caattggaca actctttcgc
     8281 gtaaccacct tcggcgaatc gcacgggctg gcgctcggct gcatcgtcga tggtgttccg
     8341 ccaggcattc cgctgacgga agcggacttg caacatgacc ttgatcgtcg tcgacctggg
     8401 acatcgcgct ataccaccca gcgccgcgag ccggatcagg tcaaaattct ctccggtgtt
     8461 tttgaaggtg ttactactgg caccagcatc ggattgttga tcgaaaatac cgaccagcgt
     8521 tctcaggatt atagtgcgat taaggacgtt ttccgtccag gtcatgccga ttacacctat
     8581 gaacaaaaat acggcctgcg cgattatcgc ggcggtggac gttcttccgc ccgcgaaacc
     8641 gctatgcgcg ttgcagcagg ggcgattgcc aaaaaatatc tcgccgagaa atttggcatt
     8701 gagattcgcg gctgcctgac ccagatgggt gatattccgc tggaaatcaa agactggtcg
     8761 caggtcgagc aaaatccgtt tttctgcccg gacccggaca aaatcgacgc gttagatgaa
     8821 ctgatgcgcg cgctgaaaaa agagggtgac tccatcggcg cgaaagtcac cgtcgttgcc
     8881 agtggcgtac ccgccggact tggcgagccg gtttttgacc gcctggatgc cgacatcgcc
     8941 catgcgctaa tgagcattaa cgcggtgaaa ggtgtggaaa ttggtgatgg ttttgacgtg
     9001 gtagcgctgc gtggcagcca gaaccgtgac gaaatcacca aagacggttt ccagagcaac
     9061 catgcgggcg gcattcttgg tggtatcagc agcgggcagc aaatcattgc ccatatggcg
     9121 ctgaaaccga cctcaagcat tactgtgccg gggcgcacga ttaaccgctt tggcgaagaa
     9181 gttgagatga tcaccaaagg ccgtcacgat ccgtgtgttg ggatccgcgc ggtgccgatc
     9241 gctgaagcga tgctggcgat cgttttaatg gatcacctgt tacggcaacg ggcgcaaaat
     9301 gctgatgtga agactgatat tccacgctgg taaaaatgaa caaaactgcg attgtgctgc
     9361 tggccctgct tgccagtagc gccagcctgg cagcgacgcc gtggcaaaaa ataacccaac
     9421 ctgtgccggg tagtgcacaa tcgataggca gtttttcaaa tggctgtatc gtcggtgctg
     9481 acacgctgcc gatacagtcc gaacattatc aggtcatgcg taccgatcag cgtcgctatt
     9541 tcggacaccc ggatctggtg atgtttatcc agcgtctgag tcgccaggtg agcaatctgg
     9601 gtatgggtac ggtgctgatt ggcgatatgg ggatgcctgc tggtggtcgt ttcaacggcg
     9661 gtcacgccag ccaccagact ggactggatg tcgatatctt cctgcaactg ccaaaaacac
     9721 gctggacctc cgcgcagctc ttgcgcccgc aagcgttaga tttagtttcc cgcgacggta
     9781 aacacgttgt cccgacgctg tggaagccgg aaattttcag cttgattaaa ctcgccgcgc
     9841 aggacaaaga cgtcacgcgc atttttgtta atccggcgat taaacaacaa ctttgccttg
     9901 atgcgggcac cgatcgcgac tggttacgca aagtgcggcc ctggttccag catcgcgccc
     9961 atatgcatgt acgactacgt tgtcctgccg atagcctgga gtgtgaagat caacctttac
    10021 cgccaccggg cgatggttgc ggggcagaac tgcaaagctg gtttgaacca ccaaaaccgg
    10081 gaacaacaaa gcctgagaag aagacaccgc ctccgttgcc gccttcctgc caggcgctac
    10141 tggatgagca cgtgatctaa tggaaacgtt taatagcctg tttatggttt ccccgctgct
    10201 gctgggagtt ctcttttttg tcgccatgct ggcgggattt atcgactcga ttgccggtgg
    10261 cggtgggtta ctcaccattc cggcattgat ggcggcgggg atgtctccgg ctaacgcgct
    10321 ggcaaccaat aaactgcaag cctgcggcgg ctctatttcc gccactatct actttattcg
    10381 ccgcaaagtg gttagtttaa gcgatcagaa actcaatatc gccatgacct ttgtcggctc
    10441 aatgagcggc gcattactgg tgcagtatgt tcaggctgat gtcttacggc agattctgcc
    10501 cattctggtg atttgtatcg gcctctattt cttgctgatg ccaaagctgg gtgaagaaga
    10561 tcgccagcgc cggatgtatg gactgccatt tgcgctggtg gcggggggct gcgtcggttt
    10621 ttacgatgga ttctttgggc cagctgccgg atcgttttac gcgctggcct tcgttacgct
    10681 gtgcggattc aacctcgcca aagccacggc tcacgccaaa ttactcaacg caacgtcaaa
    10741 cattggcggt ctgctgctgt ttattctcgg cggcaaagtg atttgggcga cgggctttgt
    10801 gatgctggtg gggcagttcc tcggcgcgcg catgggttca agactggtgt tgagcaaagg
    10861 acaaaagctg atccgcccga tgatcgttat cgtctcggcg gtgatgagtg caaaattact
    10921 ttacgatagc cacggacagg agatcctcca ctggttgggg atgaactaat gaacagtaca
    10981 caccactatg agcagttgat tgagattttt aatagctgct ttgccgatga atttaatacc
    11041 cgtctgatta aaggcgacga cgaaccgatc tatcttcctg ctgatgcgga agtaccgtat
    11101 aaccgaatcg tctttgctca tggcttttat gccagcgcaa ttcatgagat ttcgcactgg
    11161 tgtattgccg gaaaagcgcg ccgtgagcag gttgatttcg gctactggta ttgcccggac
    11221 ggtcgcgatg cccagacgca aagccagttt gaagatgttg aagtgaagcc gcaggcgctg
    11281 gactggttgt tctgcgtggc ggcgggatat ccgtttaatg ttagctgcga caatctggaa
    11341 ggggatttcg aaccggaccg cgtggtgttt caacggcggg tacatgcgca ggtaatggat
    11401 tatctggcaa acggcattcc cgaacgtccg gcacgcttca ttaaagcact acaaaattat
    11461 tatcacacgc cggaacttac ggcggaacag ttcccgtggc cggaagcgct caactgaagc
    11521 cagcgccacg ttttacaatg aggaaagaag atgatcgcgg agtttgaatc acgcattctg
    11581 gcattaatcg acggtatggt tgaccatgcc agtgatgatg agttgtttgc cagtgggtat
    11641 ttgcgtggcc acctgacatt agccatcgca gaactggaaa gtggtgatga ccactccgcc
    11701 caggcggtac atacaaccgt tagccagagt ctggaaaaag cgattggtgc cggtgagttg
    11761 tcgccgcgtg accaggcgct ggtgaccgat atgtgggaaa acctgtttca gaaggcgtca
    11821 cagcagtaag taatgtgccg gataaggcgt tcacgccata tccggcaagc aatgccagag
    11881 ctgattaccc cgccttaacc gctttacctt tcaacaactt ccgcacccat aaccgattcg
    11941 ggtttaacgc cgccagcgta ctggcatcca tcggaattgg ttcgttgctc atctgcgccg
    12001 ccagaatctc ggcacacaac ggggcagagc acaaaccgcg agaacctaaa gcagctaaca
    12061 taaaaagatc gtcataaatt ggcgcgctta ctgcctcatc tttctgctcc gccagcgaag
    12121 catattccac gagcgttacg tcataatcgg gcacgttgcc taccattggc agatgatcgc
    12181 gggtggcaca acgcacaccg cagcgcgcct ctttctcact gacatcaacc tcttttgccc
    12241 actgcgcctg cgggaaacaa tcaatcaacc gctggcgatt ctgctgctga tcgtcctcac
    12301 tgtacgccgt atcttcgctg ccgcgatgat aactggcacc aatacaatga tgttgattcg
    12361 ccggattttg tggcgtgaga taaccgtcat agcacagcac ctgcttcagc tccgctagtt
    12421 ccggcgttgt cggaatatgg ctaacctgcc cggcaaccga atacactggc agagacgatg
    12481 tttggctgaa acggctgatt tgatgcccgt tcgccagtac caccacgcta tgcgttgctt
    12541 gctgatctcc tgcaaaattc agcaaccaac agtcatcctt acgggataaa tcctttaact
    12601 ggtgttgata acgaatctgt aaaccttgct gttgcgccag tttcagcaca ttacaggtca
    12661 gttctgctgg acacagccat ccaccttgcg gatatgtaat accgccacaa tttgttacta
    12721 cacctgtaac gccttcaacc tcattcgccc cgaccgcaac agccagttct gtgggtaaat
    12781 ccatcgacag catctgtgcg attttatgct ggcttttctc atcccagcct aactgcgtga
    12841 cgccgcacca gtcatgatca aatttgacgg gcaataaatc gtaaagccga cgagcaaaag
    12901 taaacgcatt agagaaaaag cggttcaacg cctcatcgtg tttacttaag agcggataca
    12961 gcgccccctg gcgattgccg gaagcaccca gcgcgggggc ttcatccgcg caataaagcg
    13021 ttacttgcca gccgcgccgt aataacgcca gcgacaacaa cgcgctggca ataccaccgc
    13081 cgataatcgc tacttcccgt ttgctgctgc ccgtacgatt aaaccacggc gtggagcagg
    13141 ggaggggtaa tgtctgttcc atcaccccac aaagcatttc ccgtttgcgc ccaaagccct
    13201 tacgtttttg catcgtgaat ccggcgtcct gcaaaccgcg gcggacaaaa ccggcagacg
    13261 taaatgtcgc cagcgtgctg cccggacgcg ccagtcgtgc catggcgtta aacagatttt
    13321 gcgtccacat atccgggttt ttcgctggcg caaagccgtc cagaaaccag gcgtccactt
    13381 tttgattcag cgagtcgtcc agttggctgg tcagttcgtt tatatcgcca aaccataaat
    13441 ccagtgtcac gtggccttca tcgagcaata aacgatggca accgggcaag ggcattggcc
    13501 actgcgcctg aagttgttct gcccacggag ccagttccgg ccagtgtaga tgcgctaagg
    13561 ctaaatccgc acgggtgagg ggaaatttct caaaactaat gaaatgtaag cgttgtaatt
    13621 gcgcttgcgg atgtgcttcg cgaaactgat caaatgcctg ccatagtgtc aggaagttta
    13681 atccggtgcc gaagccgctc tctgctacca caaacagagg atgtggatgc tcaggaaagc
    13741 gtgcctctaa ttggttacct ccgagaaaaa cataacgcgt ctcttccagt ccgttgtcat
    13801 tggaaaagta aacgtcgtca aaatctcggg aaacaggtgt accctcagca ttaaattcga
    13861 ggttggcagg ttgtatggcg tagtgtttca cgtaagttac tcgtctgacg ggcagtctga
    13921 cgatcttaac gatgtgtgtc aggctgcgca aatttctcta ttaattggct gatcggactt
    13981 gttcggcgta caagtgtacg ctattgtgca ttcgaaactt actctatgtg cgacttacag
    14041 aggtattgaa tgaaacgtgc agtgattact ggcctgggca ttgtttccag catcggtaat
    14101 aaccagcagg aagtcctggc atctctgcgt gaaggacgtt cagggatcac tttctctcag
    14161 gagctgaagg attccggcat gcgtagccac gtctggggca acgtaaaact ggataccact
    14221 ggcctcattg accgcaaagt tgtgcgcttt atgagcgacg catccattta tgcattcctt
    14281 tctatggagc aggcaattgc agatgctggc ctctctccgg aagcttacca gaataacccg
    14341 cgcgttggcc tgattgcagg ttccggcggc ggttccccgc gttttcaggt gttcggcgct
    14401 gacgcaatgc gcggcccgcg cggcctgaaa gcggttggcc cgtatgtggt caccaaagcg
    14461 atggcatccg gcgtttctgc ctgtctcgcc acgccgttta aaattcacgg tgtaaactac
    14521 tccatcagct ccgcgtgtgc gacctccgca cactgtatcg gtaacgcagt agagcagatc
    14581 caactgggca aacaggacat cgtgtttgct ggtggcggcg aagagctgtg ctgggaaatg
    14641 gcttgtgaat tcgacgcgat gggtgcgctg tctaccaaat acaacgacac cccggaaaaa
    14701 gcctcccgta cttatgacgc tcaccgtgac ggtttcgtta tcgctggcgg cggcggtatg
    14761 gtggtggtcg aagaactgga acacgcgttg gcacgtggtg ctcatatcta cgctgaaatc
    14821 gttggctacg gcgcaacgtc tgatggcgca gacatggttg ctccgtctgg cgaaggcgca
    14881 gtgcgttgca tgaagatggc gatgcacggt gttgataccc caatcgacta cctgaactcc
    14941 cacggtactt cgactccggt tggcgacgtg aaagagctgg ctgctatccg tgaagtgttc
    15001 ggcgataaga gcccggcgat ttctgcaacc aaagcaatga ccggtcactc tctgggcgct
    15061 gctggcgtac aggaagctat ctactctctg ctgatgctgg aacacggctt tatcgccccg
    15121 agcatcaaca ttgaagagct ggacgagcag gctgcgggtc tgaacatcgt gaccgaaacg
    15181 accgatcgcg aactgaccac cgttatgtct aacagcttcg gcttcggcgg caccaacgcc
    15241 acgctggtaa tgcgcaagct gaaagattaa ttcggagtag gtcggatagg acgcgtcagc
    15301 gtcgcatccg acgatatgcg gcaatgctgc ctccggtact aaagaataag ggaaccctat
    15361 ggttcccttt ttcacatcat tgacaatcgc cgcctgtacc aggcaaactt cccgctttgt
    15421 cgatttcctt ctgaaaagac gtacgcgtta attcctgcca acgcaccgta accctgaaac
    15481 cagagagatg agacggggat actcctcgcc ttgcgctgca ttctggagta atgcatgact
    15541 gctgtaagcc aaaccgaaac acgatcttct gccaattttt cgctcttccg catcgctttt
    15601 gcggtttttc tcacctacat gaccgtaggg ttgccgttgc cggttatccc gctgtttgtt
    15661 catcatgaac tgggctatgg caacactatg gtcggcattg ccgtcgggat tcagtttctg
    15721 gctacggtgc tgacgcgtgg ttacgccggg cgactggccg atcaatatgg cgcaaaacgt
    15781 tcggcgcttc aggggatgtt agcttgtggt ctggcggggg gcgcgttgct gctggcggcg
    15841 attttgcccg tccccgcacc gttcaaattt gccctgttgg tcgtcgggcg tttgattctt
    15901 ggctttggtg aaagccagtt actgacgggc gctctgacct ggggattagg catcgttggg
    15961 ccaaaacatt ccggcaaagt gatgtcatgg aacggaatgg cgatttacgg tgccctcgct
    16021 gttggtgcgc cgcttggcct attgattcat agccattacg gttttgccgc actggcgctc
    16081 accacaatgg cattaccctt actggcgtgg gcctgtaacg gcacagtgcg caaagtaccg
    16141 gccctggcgg gggaacgtcc atcgctgtgg agcgttgtcg ggcttatctg gaaaccaggg
    16201 ttaggtctgg cactacaagg cgttggtttt gcggttatcg ggactttcgt ttcgctctac
    16261 tttgccagca aaggatgggc gatggcgggc tttacgctta ccgcgtttgg cggcgcattt
    16321 gtcgtgatgc gcgtcatgtt tggctggatg ccggaccgtt ttggcggcgt gaaagtggcg
    16381 atcgtctctc tgcttgtaga aacggtgggc ttgttgctgc tctggcaagc cccaggtgca
    16441 tgggtcgcat tagcgggcgc ggcgttaacc ggagccggat gttcgcttat ctttcctgcg
    16501 ctgggcgtgg aggtggttaa acgcgtaccg tcacacgttc gcggcaccgc actgggcggt
    16561 tacgccgcgt ttcaggatat cgccctcggc gtctccgggc cgcttgcggg aatgctggcg
    16621 accacgtttg gttactcttc ggtatttctt gccggggcga tctctgcggt gctgggaatt
    16681 attgtcacga tactgtcatt tcgtcggggt taacgaacca gccagaccag catcaggata
    16741 atcgcgacta acagtatcca cagcgcggga cgcgttgcca tattttgcaa cgtgtccatc
    16801 atcggtggaa aaggattaag cagcggttgc atatcacgcg gatcaatccc tttaacccgc
    16861 cgttgataaa gctgattaag aatatcctcg gcctgggcag aagaaagcga tgattgcgca
    16921 gaaaggccat atttttgctg gatatatgcc gataacgccg ccagttcact ggcatctaaa
    16981 ggttgtttca gcgccatctg tagtgattca agcgtcggcg tattttgctg gcttagcgtc
    17041 tgacgcgcct gcagccaggt caccagatgg ttaaacagtt tcgctggaat taactcgcca
    17101 tctttcaccc cggaaagttc cagcatcgat tgccagatct gtttgctggg ttctcccgtt
    17161 gccgccgcaa gtttggtcac cagctgtttg agcgcattgt gctccgccgg taataacgga
    17221 cggtcggtcg cctcgcgctg ctgcggttgc ggaataacca tcttcccttc ctgcaacagg
    17281 gttagaatgg tttttaattg ctccggtgag agctgattca gcggcgtctg accaaagtta
    17341 tgacggatat aatccgtgac cgcctgacga ttattcccca gacgtaaata ctcccctaat
    17401 tgcgctaaaa gctggcgggc agaatggctt ttttgcgcgg ccagcagacg ttgcgccaga
    17461 ttgtgctcag cggcagggaa gtgacgcgaa agcagcggtg aatctcccga cagaccaata
    17521 tcgtgcctga tacccgccca cagttctgct ctttgctgtt gcgtcagtga ggtcactttc
    17581 gtcattaagc tttccagcga agtacgttgc tgactggata aaggctgatt gcccgcgcca
    17641 gacggcaggt tatctccctg acctggtggt tgcccaggag gagggccgga aataggttgt
    17701 atcattacgt atccttatac ctgaaatctt cgcaagtatg cctggccgcg agattatggc
    17761 acacttgtcc ggttaactct cgtctcatac aggtaacaca aacgtgaaaa tccttgttga
    17821 tgaaaaatga aaattattta acattttgat ataaaaggat ttatatcaat tgttgtccac
    17881 gcaatgacca cattttcgag atatagaaaa agcccgcagt ttttacgctg cggg
//
LOCUS       DAZGHF010000097        17708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGHF010000097 DAZGHF010000000
VERSION     DAZGHF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.097"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..282)
                     /gene="yddM"
                     /locus_tag="ACHK3A_003415"
     CDS             complement(<1..282)
                     /gene="yddM"
                     /locus_tag="ACHK3A_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO8741459.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(282..560)
                     /locus_tag="ACHK3A_003416"
     CDS             complement(282..560)
                     /locus_tag="ACHK3A_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8741460.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            complement(746..1756)
                     /gene="adhP"
                     /locus_tag="ACHK3A_003417"
     CDS             complement(746..1756)
                     /gene="adhP"
                     /locus_tag="ACHK3A_003417"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO8741461.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            complement(1890..3587)
                     /gene="maeA"
                     /locus_tag="ACHK3A_003418"
     CDS             complement(1890..3587)
                     /gene="maeA"
                     /locus_tag="ACHK3A_003418"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO8741462.1"
                     /translation="MEPKTKRQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            complement(3744..3881)
                     /gene="sra"
                     /locus_tag="ACHK3A_003419"
     CDS             complement(3744..3881)
                     /gene="sra"
                     /locus_tag="ACHK3A_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO8741463.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(3983..4198)
                     /gene="bdm"
                     /locus_tag="ACHK3A_003420"
     CDS             complement(3983..4198)
                     /gene="bdm"
                     /locus_tag="ACHK3A_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO8741464.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            4543..4974
                     /gene="osmC"
                     /locus_tag="ACHK3A_003421"
     CDS             4543..4974
                     /gene="osmC"
                     /locus_tag="ACHK3A_003421"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO8741465.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(5030..5956)
                     /gene="ddpF"
                     /locus_tag="ACHK3A_003422"
     CDS             complement(5030..5956)
                     /gene="ddpF"
                     /locus_tag="ACHK3A_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO8741466.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLTTHGCEVRQSLTIREDEREIRCWRAL"
     gene            complement(5949..6935)
                     /gene="ddpD"
                     /locus_tag="ACHK3A_003423"
     CDS             complement(5949..6935)
                     /gene="ddpD"
                     /locus_tag="ACHK3A_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8741467.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLKINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGHISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPVLTAYGDNNHRCACWYPQQEVISV"
     gene            complement(6932..7828)
                     /gene="ddpC"
                     /locus_tag="ACHK3A_003424"
     CDS             complement(6932..7828)
                     /gene="ddpC"
                     /locus_tag="ACHK3A_003424"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO8741468.1"
                     /translation="MMLSEETSAVRPPKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(7825..8847)
                     /gene="ddpB"
                     /locus_tag="ACHK3A_003425"
     CDS             complement(7825..8847)
                     /gene="ddpB"
                     /locus_tag="ACHK3A_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8741469.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(8849..10399)
                     /gene="ddpA"
                     /locus_tag="ACHK3A_003426"
     CDS             complement(8849..10399)
                     /gene="ddpA"
                     /locus_tag="ACHK3A_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8741470.1"
                     /translation="MKRSILFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSHPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(10413..10994)
                     /gene="ddpX"
                     /locus_tag="ACHK3A_003427"
     CDS             complement(10413..10994)
                     /gene="ddpX"
                     /locus_tag="ACHK3A_003427"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO8741471.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPLAASYPLLADQFTCFISPGTQHAS"
     gene            complement(11252..13651)
                     /gene="dosP"
                     /locus_tag="ACHK3A_003428"
                     /gene_synonym="pdeO"
     CDS             complement(11252..13651)
                     /gene="dosP"
                     /locus_tag="ACHK3A_003428"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO8741472.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSMLPLK
                     I"
     gene            complement(13676..13840)
                     /locus_tag="ACHK3A_003429"
                     /pseudo
     CDS             complement(13676..13840)
                     /locus_tag="ACHK3A_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(14217..15536)
                     /gene="digH"
                     /locus_tag="ACHK3A_003430"
     CDS             complement(14217..15536)
                     /gene="digH"
                     /locus_tag="ACHK3A_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO8741473.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPTGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMVDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(15667..17202)
                     /gene="gadC"
                     /locus_tag="ACHK3A_003431"
     CDS             complement(15667..17202)
                     /gene="gadC"
                     /locus_tag="ACHK3A_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO8741474.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(17358..>17708)
                     /locus_tag="ACHK3A_003432"
     CDS             complement(17358..>17708)
                     /locus_tag="ACHK3A_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO8741475.1"
                     /translation="AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
BASE COUNT         4360 a         4552 c         4427 g         4369 t
ORIGIN      
        1 ttgcgtaacc agacggcgca gtcgcgacac atccaccgtt ttttcagcct ctgccaggct
       61 ccaggcgttt tgcagattca gccacatttg cggcgaactg ccgatcacca cggaaagttt
      121 aatcgccatt tccggagtga gcgccgcttt tccggtcagc aatcggcttg ccgttgaggg
      181 cgcaatttcc attgctctgg caaactcgcg caggctgaca ttaagttcgt ccagtgattc
      241 ctgaataatg tcccccgggc ggggatgatt ggccattttc atcagtgata gtcctcgtaa
      301 tccagtatgt atgcatcgcc attgacgaat tcaaatgtga ttcgccagtt gcccgagaca
      361 gatatcgccc aaattccatc gcgatcgccg cttaacgggt gtagtcggta accgggcata
      421 tcgatatcag caagacagct tgctgcatca atcacagcga gtcggtggcg taatcgtttg
      481 acttgcgtcg gtataacgcc ggaggttttg ccgagaagaa acaagtcacg caatcccttg
      541 tgtctgaaat tcattatcat ctcttcatcc ttgttccttc aaaaggaaca ctatcactat
      601 tgttccgtgc agcgcaacaa atttcgctac gtttttgcga attgtgaggg gaaatatggg
      661 ggaggaaaag aagaagagtg aaattgcgga aagcgtctcg caaaacaggc tgcgacccga
      721 acatggcagt cgcagcaaag gcctcttagc ggcggaaatc aatcaccata cggccacgga
      781 ttttgccttc ttccatctcg gtaaagatgg tgttgatgtc cgctaacgga cgcagggcga
      841 ctttcggcac cactttgcct tcggcggcaa actggaaggc ttcggttaga tcctggcgcg
      901 tgccgaccag cgaaccgacc acttcaatac catccagcac aaggcgtggg atgtccaggc
      961 tcatagactc cggcggcaga ccgacagcca caacacgacc gcctgctcgt acagcatcaa
     1021 ccgccgagtt aaacgcagct ttagctaccg ctgtcaccac cgcggcgtga gcgccaccgg
     1081 ttttttcttg cacaattttg gcggcgtctt cagtgcgtga gttaatcgct aaatcagcgc
     1141 ccatttcggt tgccagtttt aactgctcat cattgacatc aatggcgatc actttggcgt
     1201 taaagacatt cttcgcgtat tgcagggcga ggttacccag accgccaaga ccgtagatag
     1261 caatccactg accgggacga atttttgaca gtttcaccgc tttataggtg gtgacccccg
     1321 cacaggtaat gctgctggcc gccgccgagt ccaggccatc tggcactttt accgcgtaat
     1381 cggcgaccac gatgcactct tccgccatcc cgccatcaac gctgtatccg gcatttttaa
     1441 ctgaacggca gagcgtttcg ttaccactgt tacagtattc gcaatgaccg catccttcgt
     1501 aaaaccacgc cacgctggca cgatcgcctg gttttaatga ggtgacacct gggccaactt
     1561 ctgccaccac accgatgcct tcatggccca gaattacgcc ggttttgtca ccaaaatcgc
     1621 cattcttaac atgaagatcg gtatgacata caccacaaca ctccattttc agcagggctt
     1681 cgccatgttt cagtgagcgc agtgttttat ccgtaacgtc aacatgatga tccttcgtaa
     1741 caactgcagc cttcatagtt cctccttttc ggatgatgtt ctgcatagca ggtgaggcaa
     1801 atgagattta ttcgccacta cccagtatgg atgagatctg aaaaagggag aggaaaattg
     1861 cccggcagct ttcaccaccg ggcgcaggct tagatggagg tacggcggta gtcgcggtat
     1921 tcggcgtgcc agaaattatc atcaatggcc tgttgcagag cttcggcaga cgttttcacc
     1981 gccacgcctt gctgctgcgc cattttgcca accgcaaagg caattgcgcg ggagactttc
     2041 tgaatatctt tcagttccgg tagtaccaga ccttcgccgt tcaggaccag cggcgaatac
     2101 tgagcaagcg tttcacttgc cgacatcagc atctcatcgg tgatacgtga cgcgccggaa
     2161 gcaataacgc ccagcccgat gcccgggaaa ataaaggcgt tgttacactg ggcgataggg
     2221 tagattttat ctttccatac cactgggtta aacggactgc cagtggcgac cagcgcgtta
     2281 ccttcagtcc aggcgataat gtcctgcggt gtggcttcca cgcgtgaagt cgggttagac
     2341 agcggcatca cgatcggacg cggacagtgt ttatgcatct cacggataat ctcttccgta
     2401 aacagcccgg tctgtcctga gacgccaatc agaatatctg gttttacatt acgcaccaca
     2461 tccagcagtg acagcacatc gctgtcggta tcccagtcac tgaggttttc gcgcttctgc
     2521 accagtttgg tctggaaagg cagcaggttc ggcatcttgt cggtcagcag gccaaagcga
     2581 tcaaccataa agactttctg ccgcgccgct tcctcgctta atccttcacg ctgagtctgg
     2641 gcgatgatca tttcggcaat gccgcacccc gcagagcctg cgccgaggaa gacaatcttt
     2701 ttctcgctca gttgaccacc tgccgcgcgg ctggctgcga tcagtgtgcc gaccgttacc
     2761 gccgcggtgc cctgaatgtc atcgttaaaa gaacaaattt cattgcgata gcggttaagt
     2821 aacggcatcg catttttttg tgcgaagtct tcaaactgca acagtacgtc cggccagcgt
     2881 tgtttcacag cctggataaa ttcatcaacg aattcatagt attcgtcgtc agtgatacgc
     2941 ggattacgcc agcccatata cagcggatcg ttaagcagct gttggttgtt cgtcccgaca
     3001 tccagcacca ccggaagggt atacgccggg ctgatgccgc cacaggcggt atagagcgac
     3061 agtttaccga tcggaatgcc catcccgccg atgccctggt caccaagccc cagaatacgt
     3121 tcaccgtcag tcaccacaat cactttaata ttatggttcg gcacgttttg cagaatatcg
     3181 tccatattgt ggcggttctg gtaagagata aacacgccgc gtgaacggcg gtagatctca
     3241 gaaaaacgct cacaggctgc gccgacggtt ggggtataaa taacaggcat catctcatca
     3301 agatgattgt ttaccagacg gtagaagagg gtttcgttag tgtcctggat gttacgcagg
     3361 tagatgtgtt tgtcgatttc ggttttgaat ccctgatact ggatccatgc tcgttccgct
     3421 tgttcttcga tggtttcgac cacttccggc agtaacccca gcaggttgaa gttacggcgt
     3481 tcttccatgc tgaaggcgct gcctttattc aacaacggaa attccagcag tacagggcca
     3541 gcgtaaggaa tataaagcga acgctgtctt tttgtttttg gttccatgtc actcactctt
     3601 ttttgaatat ccatccctgg cacgctttta tcgtctttgc tttaccgcca gggcgtcggc
     3661 cccaagtata aagcagataa aaacaaaaac accattgcgc aggcaatggt gtttaatcgt
     3721 cattgaggac tgatggttat gaattacttt tcagcggggc gttttctgcc tgttgggtta
     3781 tttaccacgc tggatttgtc accttcggta actattttgc gctggttaga aattttatgg
     3841 tccagtccaa gaatatgacg tgcctgacgg ttcgatttca ttttaactcc tcaatcctgt
     3901 tactagtttt aaggacaaca tcgccgtagc gaagaaacac gtgctaaacc cctaaattag
     3961 gttgccgatc aagcatagca ccttaaagcg tagggtgctg accactgacc acataattga
     4021 tcgtttgctg gtagatatca ctgaggatgt cgttatcaga tgcttccacc catttggtca
     4081 gctccatgag aatgtcatct tcagtgacaa caccgtgctc tgcccgaagg ccttgctcaa
     4141 tggcattaac cagagcgggt tctgctgttg aattttctgc ctgataataa gtaaacatag
     4201 tgattctccg tgtctgtgta tttatggtgt ctgctacgga tcgcagattt ataaagcaca
     4261 ttcagcatgg caaatatttg ccgcttcgtt gttaagatta gtcctggttg atgattttta
     4321 tattttaact ccatgatatt gataggtatt attattgtga tgagttgatt gatattgatg
     4381 caatatctct tgagttatat atttccggtt atctattgtg gaaatttaat ttaagtgcag
     4441 gaattatatt ttcgccggat tttattcgga ttatcctgct tatcctcgtg ctgtttctca
     4501 cgtagtctat aatttccttt ttaagcccac aggagagcaa caatgacaat ccataagaaa
     4561 ggtcaggcac actgggaagg cgatatcaaa cgcgggaagg gaacagtatc tactgagagc
     4621 ggcgtgctga accaacagcc gtatggattt aacacgcgtt ttgaaggcga aaaaggaacc
     4681 aaccctgaag aactgattgg cgcagcgcat gccgcatgtt tctcaatggc gctttcatta
     4741 atgctggggg aagcgggatt cacgccaaca tcgattgata ccaccgcaga tgtgtcgctg
     4801 gataaagtgg atgccggttt tgcgattact aaaatcgcac tgaaaagcga agttgcggtg
     4861 ccgggtattg atgcctccac ctttgacggc attatccaga aagcaaaagc aggatgcccg
     4921 gtttctcagg tactgaaagc ggaaattacg ctggattacc agttgaaatc gtaaagcgtt
     4981 gccggatgac gcgtcaggcg tgtgattgcc tgatgcgttg ttagcgtctc tacagcgccc
     5041 gccagcaccg gatctcacgt tcgtcctccc ttattgttaa tgattgccgg acttcacatc
     5101 cgtgggttgt cagagggcag cgttcgcgga aaaaacagcc ttgtggcaac gtgcggtttc
     5161 ctggtagatc cgttttacgt aatgcccatt cttcctccag cggtttatca atggcgggga
     5221 gggaatccag caataatcgg gtgtatggat gtgccggagc tgtcagcacc tgttgcgcgt
     5281 ctcctagttc cacaatctgt ccgagataca tcaccgccac ccgatcgctc atatgacgta
     5341 tcaccgagac attgtgtgaa atcagcacat aagtcagctc gcgattttcc tgtagcgtta
     5401 ccagtaaatt gaggatctgc gcctgcacgg agatatccag cgccgaggtt ggttcatcaa
     5461 gcacaatcac gtcaggctgc gaagagagtg ctctggcaat ggcgatacgt tgccgctgcc
     5521 cgccggaaaa cgcatgaggc aagcggtcga gatactccgg acgaataccc acctgcacag
     5581 ccagctcctc tgccaacgct cgccgctgtt gttcactact acgcttagca atccagagcg
     5641 gttccgtgat gatgcgccac accggtaagc gcggattaag cgaagagagc gggtcctgaa
     5701 acaccatctg cataatgcgt tgtgatcctg aacggatgta ctgcccgtgg ctcggttgca
     5761 gcatacccat taaaagctgt gcgagggtgc ttttgccgca gcctgactca ccgacgatcc
     5821 ctaaggtttc accacgacgg atctgtaaat caataccatt gatggcatga acatgttccg
     5881 tagttttacc aagccagttt ttacgggccg ggaaattgat atggacgtcg cgtaacgtta
     5941 ataatgtatc agacactaat gacctcctgc tgcggatacc aacaggcgca gcggtggttg
     6001 ttgtcaccat acgctgttag caccgggacg ttttcacact gtgcacctgc cgcatagcaa
     6061 cgatcgcgaa aagcgcagcc gtcaggcaaa tgggtgaggt ttggtaccgt ccccggaata
     6121 gcgggtaatg gctggcgcgg tattccatgt tccggtgcgc attgcagcaa accaatggta
     6181 tacggatgcc ggggatgatg gataacgtct gccgtcacgc cgctctcaat cacgcttccg
     6241 gcatacatca cgtaaacgct atcgcacagt tgcgacacca cggccatatc atggctgatg
     6301 aacagtaccg cagttccact ggcgcgggct ttatgtttaa gcagacgcag tacctgcaac
     6361 tgtaccgtga cgtccagcgc cgtagtcggt tcgtcggcaa taatcaattg cggctcgcag
     6421 gagaatgcca gcgcaatcat tacccgctgg cgcataccac ctgaaagctc aaacggatag
     6481 cgcgacataa cttccacggc atccgggatt tgcatctctt ccagcagggc aatcgcttta
     6541 gctctggctt cccgacgact tattggttga tgatggcgga tcacgtccat catctgaaga
     6601 cctattcgac gtgtcggatt aagggcggtc atcggttcct gaaagatcat cgccactcgt
     6661 gcgccgcgcc actgacgaag ctgcttttcc cgagcgttaa gaacatcttc tcccagcagt
     6721 gaaatatgtc cccgatgtac gcaataactg cccgtcggta gcagacgcat aatcaacatt
     6781 gcagtgactg atttacccga gccggattct cccaccagac cgacaatttc accgcggttg
     6841 attttcaatg acacattgtt gagcgcgtga acgtcgccgt taaaaccggg gaaactcaaa
     6901 tgcagttgtt gaatgtccag aacgggttgg gtcatgactg ctttcctcct gctttcgggt
     6961 caagcagatc gcgaatacca tcgccaaaga gattaaaccc gacggcggta agcaaaatcg
     7021 ctgcccccgg aaatgcgcaa taccaccatt gatcgagcac atagttgcga ccattcgcca
     7081 ccatcgcccc ccattcagca ctcggttgtt gagcaccaag gccaataaat cccaacgtgg
     7141 cggccattaa aatcgcgcta ccgatatcca gcgatgcctg cacaatcagc ggcggcaggg
     7201 agttacgtaa aatatgccag ttgatcagat gccagcgtga cgcaccaaag gttttcgccg
     7261 cctgaacata ggtatactgg cgaacaacta atgtttgccc ccgcgccagg cgcacataaa
     7321 agggaattcg cacaatagca atcgccagca tggcgttaaa caaactcggc ccgagagcgg
     7381 ctgccagtgc cattgtcagc accagcgaag gaatcgacag cataatgtcc atgatgcgca
     7441 tgataatcgc gtctgcgcgt ccgccaagca caccggatag acacccgagt agcgacccca
     7501 tcatacctgc aatggcgacc accactaatc cggcgagaat tgattgctga ctgccgacca
     7561 gtacgcggct gaacagatcg cgtcccactt catcggtgcc aaaccagtgc gctgcagaag
     7621 gcggcaaaag gcgggcggtt aaatcaatgg cgttgggatc atgcgtcgcc agccacggtg
     7681 aaaaaatcat catcaatagc attaatacaa tgatgactgc gccggtcacg gtaagcgggc
     7741 tgcctttcag catccatacc agttttgcac cgttaaatcg cgtttgtttt ggtgggcgca
     7801 cggctgacgt ttcctcgctt agcatcattc accacctcca cgtccgatac gcggatcaat
     7861 ccacaaatag agcaaatcca ccaccaggtt caccagcaca taagcaaacg aaaccacgac
     7921 ggcaaagccc atcactgccg ggaagtcgag cgcctgtatt gatgttacta cccatgcacc
     7981 cattccgggc caggcaaaaa cggtttcggt gagcactgcg ccatacaaca aatcgcccag
     8041 cgccagtccc aatacggtaa tcgaggggat caacgcattg ggtagcgcat aacataaaac
     8101 gatataccag ccgggcaagc cgctggcccg ggcggtacga atgtaatctt cactcaattg
     8161 ttccagcata gctgagcgga tctggcgagc gacaattccc aggtgaacga acgccagcgt
     8221 taatgccggt aagatgagat gttgcagcgc attgaagaag acttcaccgt tgccttcaag
     8281 cagcgcatcg agcagataaa agccggtaac gtgcgttggt ggatccagcc agtcatcaag
     8341 ccttccgccg ccgggaagaa tttgcagatg accataaaac agcacaatga cgcccagccc
     8401 aagccagaac gcaggtgtgg agattccggt aatggccatt attcgcacca gatgatccag
     8461 ccagcgattt cgccagactg cagagagtat gcccagcggg atgccaatca gaagtgccag
     8521 cagcagggca caaaaagcca gttccagcgt cgccgggaaa aatatgcgca actcttccag
     8581 caccggacgc ccggtacgaa tggatgttcc caggtcaccc tgaaacaggt cgctgacgta
     8641 gcggtaaaac tgtacgtaca gtggctggtc cagtcccagt tgctggcgaa tattatccac
     8701 gatagcatcg ctggcgcggt cacccgccag taaccgcgcc ggatcgccgg ggatcaggtg
     8761 cgagataata aacgttatca cacagacgcc cgccaccacg agcaccagcc cccagcagcg
     8821 ttggcgtaaa atactccaga aggtcacgtt atttactcat ggtattgata ttgaagacct
     8881 gttccagcat gggattgaac acaaagcctt tcacctcttt gttcatcgcc agttggtagt
     8941 ttttctggaa caggtacaca taagcagcgt catcaatgac gattttctgt gcctgctggt
     9001 agtcccgggt acgctgcgtc tggtcggtgg tcgctagcgc attgcgcagt agcttatcga
     9061 cctcactgtt ttcatagaac gagcggttac ccggcagacc ttttttgtct gactcaaacc
     9121 agtaattcat aaacatatac gggtcggcaa aatccggact ccagttgcca atcgcgatgt
     9181 cgtaatcacc tttacccact ctgtcgcgca tggtggcgtt cgccagcttt tccagcttca
     9241 cattgatgcc cagtttgttg agactggatt gtgtcgccag agcgatcggt tcccagttcg
     9301 gatcattatc agaatagaga aacgtcaggc tggtgggttt gctcgtcact ttatcccatt
     9361 cggctttggc tttcgtttcg tcatggttgt attgcattgc cgtcgcatcg tatccccaca
     9421 tgccttccgg aatcgggccg cgcatttgtt ttccgttacc actcagaatg ccgttaacca
     9481 tgccctgata atcggtagac caggaaatgg cccgacgcag atccgcctga ttaagtggcg
     9541 ctttgctgtt attcagatac agataggtga cgcgcagtga cggatactct gccacattga
     9601 ctttattttc ctgcttcagg gcgttgagtt gatccaccgg cagcgcatcg gcaatgtcaa
     9661 tgtcgccacg ggagagctgc aggcgacggg aggcactttc accgataatt tttaccgata
     9721 ctcgtttaaa gttcggttta ttgccggggt aatgcggatt tggcaccaga actaattgct
     9781 gacctttttg ccagcttttc agcataaacg gtccggagcc agcggtattt tgcgcgagga
     9841 aaccacgggc atcatccgcc gcatgctcct ttaagaccgc cggattgata atggatgcac
     9901 cgtcattcgc cagcgtgtag aggaacggtg cgaatgggtg gctaagggta aacttcaccg
     9961 tatgttcgtc gggagcatca atctttaaat ctttgggaaa tgcttctgct ggcccctggc
    10021 cgatttttag taaccgctca aaagagagtt ttacggcttc ggcagtgaca ggtgtgccat
    10081 cggcaaattt agcattgtcc ttcagggtga acgtccactc tttttgatcg tcagacgctt
    10141 tccagctact agccagatcg ccttcaacgt cggttgagcc tttatcaccg tccgttttgt
    10201 actgaaccag tcgctgataa gacgggtagg tcactgtcca gtcgttatta tcaattgtca
    10261 ccgctgggtc gagggtttgt gggtcggcag ctttaccgat caccagcata tctttcggta
    10321 cggcggcgtg cgcaaccggc attgttgtgg caaggacgat cgcgagcaat gtaggacgaa
    10381 acaaaatcga tctcttcatg acagtttcct gattagctgg cgtgctgtgt gccaggggat
    10441 ataaaacagg tgaattgatc agcgagtagt gggtaactcg ctgccagggg taattcgaag
    10501 tgccaccatt cgctcgggat accgacaaag ccaccgccag tcataatcgc attcagcaac
    10561 aggcgattgc gctgagcggc gggcgggacg gaagggtgat aagcatggga acgttcgtgc
    10621 atttcatcga accctgcacc catatcgagg atgttcccgt gctcatcacg aagcgtcagg
    10681 tcgatcgccg tgccacggct gtgattagaa ccgaccgtca catcaacaac atattgcggg
    10741 tctgggcagg cttgccacaa catcgcctgt gcttgttgtg ggcgatacgc atcgtaaatc
    10801 accagttgta agcctgacag ctgggcgata ctgatacttt tcgccagcgc agtaatcgca
    10861 tccttgtgta acagacaacg cgcttgctga taaatagctt tacctgtgat gttatcagcg
    10921 caggcgtatt tcaattcgat ctccagatca gggaagatca cggctaaatc aaccagttcg
    10981 gtggtatccg acataaacga ttcctgtata agacaaaaat tgctgcgctt tccgcttatg
    11041 cagatctcat gccacgccgg gataagcgcc agaatgctgg cttaaagtta tattttttaa
    11101 cctttgatca acatttgtgc agcgtagtgc agttttggtg caagagagga agttaaggaa
    11161 ggaatctccc ggaatcgtag ctgaaatcac agtatttaag tgacagtgtc acgttaaatg
    11221 aaaacccgcg tgtgcgggcg agaggaattt gtcagatttt cagcggtaac atactgctca
    11281 tccagcctgg aatttcttcg gcgggcaggg ggcgggaaaa gaaatatccc tgaataacgc
    11341 gacagtggat cttgcgtagc atctcaaatt gctctttggt ttcgacgcct tccgccacga
    11401 cggttaaatt aaggctttgc ccaatgctgg taatggcttc aagtaaggca aggatgcgtt
    11461 tttcggtcag acaacgatcg acaaaacttt tgtcaatttt gatttccgtt accggaagac
    11521 tgactaagcg ggataatccg gaaaagcccg tgccaaaatc atctaccgat aaaccgatgc
    11581 ccatatcgcg caggatctga atgcgtttaa agatttcggt atcgtgttcc atcatcatgc
    11641 tttccgtgat ttccaccgtc agctggtggc cgtcgatacc ccaggcttgc attgcatcag
    11701 acacctgatt aggcagctga ttactgcgaa agtgcagcgc cgacaagtta acggataacg
    11761 ccgggatatg aatattctgg ctacgccatt ctgctaactg acggcaagct tccgcgatga
    11821 cccagcgccc aatattttcg atttcaccga tctcttctgc gagaggaata aaccgtgaag
    11881 ggggcacatg accatgcagg ggatcgtgcc agcgagcaag ggcttcgatg ccgtacagtt
    11941 cacccgtttc tgcgaagatt tgcggctggt aaaccagttt cagttggtta ttgctaattg
    12001 cttctttcag cgccgcgcct aaaaccaaac gctcttttac catttcgttc atcgccgggc
    12061 tgaagaactg ccagccgtta ccgccatttt tgcgaataaa atccattgca ttgtgagcag
    12121 tggagagcag gtaatcgcgg tttttaccca cgtcgtagct gatgccaatg ctcaaggtga
    12181 gcgggaatgg tttatcgtca atcattatcg gcttgctgac cacattccgt agctcatcgg
    12241 cgatctgcgt aatgttactg acgtcgtttt ccaggctcac gaggacaaac tgcgtacctt
    12301 cgatacgaca gagatattga tccggtttga gtttttcacg aaagcgattg accacttcca
    12361 gcaatgcctg atcggcccac gcatagccaa ggctatcaat cacatcctga atatggtcaa
    12421 caccgatgag atacaccacc ggagagacgg ctttgtcgac caggtcatcg aggtaattgt
    12481 gcaggttatt gcgatttggc agaccggtca tcggatcaaa ttggatgagt tgttcaatat
    12541 gctgacggct tttttcctgt tccagcgcca gcgcggccat atgctggctg atatctgcca
    12601 cgcgttcgat aaaggcgctg gtttctgctc ctgacgaggt tttaatttgc aggatccccg
    12661 caggcgcacc atcacgctga cgaatggtcg ctgaccagct ttgcgcattt tgaacttctg
    12721 caccgtgcga agatgacgcc cagtgtatcg gcatcccgtt gcgcagcgcg aacagcgaaa
    12781 catgcgattc gttgagtaca gattcgatgt tacgacaaat gatttccccc atttcatgaa
    12841 atggcgggct gctgcacatg gcggcgagaa tattgccttc aagctggcgg atttgccttt
    12901 cttcggtgat atccgagaat gtcattacca ggttctgcag atgcgcgagc acgtcataaa
    12961 ccgggctgat agaggcttta atccagattt tttcaccggt gcgcgtcaac agcagaaatt
    13021 cgtcctgatc gcgggcggtt ttccatagca actgctgtaa acgaatacgg ttatcagcag
    13081 ggaattctgg aatgttcagg agtgtatcgg gctgcatacc gctggcttcg ttaatgcagt
    13141 aaccaaacat ttcggtaaat gcgcgattgc actgcacaat atggcgttcc ggatcgagga
    13201 caatcaccgg tcggtcgaga tggtcaacgg caataatcaa ttgtcgggtc tgttcttttt
    13261 gcgccatttc tacgctggca tcccgtacca gcgccaggta ataaactttc ccctcggcgc
    13321 tcactttcga tagcgcaaaa cgggtccaga ttttactgcc gtcttttttc tccagctgca
    13381 gctcccgact catcccctcg acgcgagctt taccaccttc acggttgtga cgaatgtatt
    13441 gaggatgcgc aggacgcaaa tcccgcggaa tcagcatatc aatgttattg ccaatgactt
    13501 cttcacgttt gtacccccag agcttctctg cggcggggtt gaaaaacatc acttcatcat
    13561 tttcgttaat taacaccgcg cccatcatat tttgctcaag ggcggggaaa aaaatgccat
    13621 cggcggcatt atccgcatcg gttagcttca tgattacctc tgcatcctgg cgcatctaaa
    13681 gactggcttt acagagttca acacgggttc tacctcgtct tttggcgata tacagagctt
    13741 catcggctat ttgaatgagg cgctcatagt caggatgacc attaaacatg gcggcaccga
    13801 tggaaagtga gagggcaata tcctcgccgt ttgcggcttt aaaataatgt cgacctttat
    13861 ggttaaaccg caggccaaaa tcaaccaaac acaacgtgcg agatcgacta actgcaccat
    13921 attctcctga aatatgaaga tatactgaaa agaaataagc gacttaggac agtttcaatc
    13981 tacgctactg ttcttgagaa gagtatagcc catcgtaatt atttttcggt gacagcgaat
    14041 atcgtatgtt ttttcatatt catacatttt tattagggga tttatgactg tttaactaag
    14101 tgtggttaat tgcgtagtta atttgactta agtaagcatg attactagtg ggatatttca
    14161 agagggtaac aagccggggg gaaagcaccg gcttgttaca aagtaagaat gggagtttaa
    14221 ctgccccaac gactttgcag atagctgacc gcttgttgag tctgtggctt attcagatag
    14281 tcctcacgga acaagattgt gccgctaatt tctggcacgg catcgttaag atcgagctgc
    14341 tttttcagtt ccggtacgcc gccgttaatc atccagtctg gctctatctt tgaaggttca
    14401 cccactttat agaaggcgat accgatatac aggcgggtcc tggtcggttt aacgacatcc
    14461 gcccaccatt ttgccaaaac gtcataacgt gcggcactcc gtgagaacgg ccagtaaatt
    14521 tggggagcaa tgtaatccag caatccttgt tccacccatc gacgggtgtc agcgtaggat
    14581 tcgtcatagg ctgccgcgcc tcgggtatcg gaaccgagcg gatcgtgtga tcggttacgc
    14641 cacacgcctg ccgggctaac gccaaattcg acttccggct taatgctttt aatagtgtgc
    14701 gatacctttg caattaactg ctgagtattg ttgcgccgcc agtctgcttt tgacgcaaat
    14761 gcgcctccgt atttgcggta cgtttcgtta tcatttagcc gtgaacccgg tgactcagta
    14821 tagaaatagt cgtcaaactg cacgccatct accggatagc gggaaaccac ttccgcgact
    14881 attgatgtga tccagtcctg aacttcaggg atgcccgggt cgaggacaaa gcgatcgccg
    14941 gacgttctga tccagtcgcg gtgttgcaca tagacgctcg ctggttgttg agacagagtg
    15001 ctattcagtt ccctgatagt accgggcttc gtattaaccg atacgcgata ggggttaaac
    15061 caggcgtgta ctttcatccc acgcttgtgg gcttcgtcga gcataaattg cagcggatcg
    15121 taacctggat tttcaccaat ctttccggtc ataagatcgg accacggcaa aatttttgat
    15181 ggccacaggg cggtaccgtc cggcttgacc tggaaaaaga ccgtgttgat gccgagacgt
    15241 tgcagatgat ccagtttgtc gaccatcgcc tgttgttgta cacgggcccg gctggtgggg
    15301 ttactaatgt taaccgagga aaccggtggc cagtcgaggc gagacactgt ggccagccag
    15361 atgccacgca tcggttgtga cgattgttgc gtcgtagctg gtggcttcga acctgttggt
    15421 ggtgtcacca ttgactctgg aggcgtgctt ttgcaactac acagtaaaag tgccagtgca
    15481 accagtatcg ctggtcttct aatcgctaat ttctcgtttc gggagcagat atccatatgt
    15541 gctggtttcc ggtaaacaga tgtgcgctca ttctcgtact tatcccggtt aagtcaatac
    15601 gacaacaagc acgaaaaagg gagcgatgaa ttatcgctcc cttgtcttat aaccattcag
    15661 acatggttag tgtttcttgt cattcatcac aatatagtgt ggtgaacgtg cacgcgggtg
    15721 caggaagaag tgaccttttg gtgcgttctg actgttgatt ggctccagag tgacgccggt
    15781 attcgctttg cttttacgat catgaacagc atagagaata aagggcaggg caagtaccac
    15841 caggaaactc acgaccagta attcaacata catatcggta gagtcaccct ggatgttatc
    15901 cggcggcagg aaggaaacaa taaacgccat aattgaagtc agcagaccga caatagccac
    15961 gaccagtttc acccctttac caccagggat attaaatgtg cgttttaagt caggatgttt
    16021 aagaaccaac acaatgtagc caataaacag catgaaataa gcacacagat aaatcaccac
    16081 cgtcagcgcc agtgcgatca ggaaggacat gttgttaccg ccaccggtat tggtgaggat
    16141 gatcaacgcg atagaagtaa tcaccaactg cgaaatgacc agcgttaccg gtacgccatt
    16201 tttgttcatt ttagcgaatg ccgctggcag caggtttttc tgcgctgtga catacatccc
    16261 gcgagaagga ccaacaatcc aggaagcgat ttccgccaga acacccagca acagcagtgc
    16321 ggagatcacg cgaaccgtcc actcaatttc cggtgccaca tgtgacatca gaacggtaaa
    16381 ggtttgcatt acccctgcgg agaggttgat ttcattaccc ggaatgacca tcgcaataga
    16441 caaaccgcca acagagctta agcagattgc cgccaccatc agcagtaaca tagccagtgg
    16501 atagtcgcgc ccgggattgc tcatttcatt gacgtgggtt gcggaagctt caacgcccat
    16561 ataactcaaa atgaaggcaa caaatacaac cagggtgccc actttagaga agtcagggaa
    16621 gaaggtcttc gaatccattt cgatagcaac gggggcaccg gagtgcagat aaatagccgc
    16681 taatgcgatc aaaataaatg caggtaacag gataccggcg aagaagccaa ctttagcaat
    16741 tcgcgccgtg tatttcgtgc caccaaactg cgttaatgcc agcgcccaaa gaatgatgag
    16801 tgctgcaata gttttggtaa tggggtcttc attcagcgct ggccatttca ggatgtagga
    16861 gagtgcccct aacacgaaat agagcatcgg aataaaacca atggcgattt gcagatagcc
    16921 aaatgagatc gctgcaaatc cccatctcgg ccccagagta tttgataccc aggcgaagac
    16981 gccaccttct tcccagccgt cgacggtagc catttccgca gcacaaagtc ccacgggaat
    17041 aaaccataaa atcccgccta atagcaggaa gaagactaat gaaaagcccg atgttgcgaa
    17101 ggtagggtat tcataaacag ccattaccat cgatgccgtt atggcaaaaa atccgagtaa
    17161 tgtgagctgc ttagctttac ctgtctgtac tgatgtagcc atattatccc cctaaaacgg
    17221 tattcctgtc ggaaccgcac ctgttttgtt ctgaagcgta ttcagaacaa tattttccgt
    17281 tgctaatgcc agtgaacaga ctttggaaat tgtcccgaaa cggcttcgtt tcgggacacc
    17341 gttaccgtta aacgttatca ggtgtgttta aagctgttct gctgggcaat accctgcagt
    17401 ttcgggtggt cgctgagata tttcagggag gctttgtagt cttccagcaa cagttcagca
    17461 aagtccattt cgaagccgcg acggcacata atgcgcatca ccacgatgtc ggtggcttca
    17521 ccgcctagag tgaaagccgg aacctgccag ccgcgcagac gcaaacgttc agagaggtca
    17581 tacagggtgt atcccggatc ttcaccatct ttcagtttga agcaaaccgc cgggatgcct
    17641 tcgtccgggc gacccgtaca gatgaactca tacggcccca gtttggcgat ttcatccgcc
    17701 agataagc
//
LOCUS       DAZGHF010000098        17614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGHF010000098 DAZGHF010000000
VERSION     DAZGHF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.098"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            355..1143
                     /gene="fabI"
                     /locus_tag="ACHK3A_003433"
     CDS             355..1143
                     /gene="fabI"
                     /locus_tag="ACHK3A_003433"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO8741476.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1287..2414
                     /gene="yciW"
                     /locus_tag="ACHK3A_003434"
     CDS             1287..2414
                     /gene="yciW"
                     /locus_tag="ACHK3A_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO8741477.1"
                     /translation="MEKRHITGKSYWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARSIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFATCMNSSNEYKKISALLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            2482..4416
                     /gene="rnb"
                     /locus_tag="ACHK3A_003435"
     CDS             2482..4416
                     /gene="rnb"
                     /locus_tag="ACHK3A_003435"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO8741478.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDLVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(4493..4597)
                     /locus_tag="ACHK3A_003436"
     CDS             complement(4493..4597)
                     /locus_tag="ACHK3A_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741479.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            4651..6636
                     /gene="pdeR"
                     /locus_tag="ACHK3A_003437"
     CDS             4651..6636
                     /gene="pdeR"
                     /locus_tag="ACHK3A_003437"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO8741480.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(6618..6746)
                     /gene="ymiD"
                     /locus_tag="ACHK3A_003438"
     CDS             complement(6618..6746)
                     /gene="ymiD"
                     /locus_tag="ACHK3A_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO8741481.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIITEYIAKYIYSSTYARFR"
     gene            6784..6957
                     /gene="yciZ"
                     /locus_tag="ACHK3A_003439"
     CDS             6784..6957
                     /gene="yciZ"
                     /locus_tag="ACHK3A_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO8741482.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            7047..7796
                     /gene="yciT"
                     /locus_tag="ACHK3A_003440"
     CDS             7047..7796
                     /gene="yciT"
                     /locus_tag="ACHK3A_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO8741483.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEIILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            8065..8283
                     /gene="osmB"
                     /locus_tag="ACHK3A_003441"
     CDS             8065..8283
                     /gene="osmB"
                     /locus_tag="ACHK3A_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO8741484.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8409..8735)
                     /gene="yciH"
                     /locus_tag="ACHK3A_003442"
     CDS             complement(8409..8735)
                     /gene="yciH"
                     /locus_tag="ACHK3A_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO8741485.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(8735..9472)
                     /gene="pyrF"
                     /locus_tag="ACHK3A_003443"
     CDS             complement(8735..9472)
                     /gene="pyrF"
                     /locus_tag="ACHK3A_003443"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO8741486.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAARFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(9663..10832)
                     /gene="lapB"
                     /locus_tag="ACHK3A_003444"
     CDS             complement(9663..10832)
                     /gene="lapB"
                     /locus_tag="ACHK3A_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO8741487.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(10839..11147)
                     /gene="lapA"
                     /locus_tag="ACHK3A_003445"
     CDS             complement(10839..11147)
                     /gene="lapA"
                     /locus_tag="ACHK3A_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO8741488.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            complement(11296..12060)
                     /gene="pgpB"
                     /locus_tag="ACHK3A_003446"
     CDS             complement(11296..12060)
                     /gene="pgpB"
                     /locus_tag="ACHK3A_003446"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO8741489.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            12230..12820
                     /gene="ribA"
                     /locus_tag="ACHK3A_003447"
     CDS             12230..12820
                     /gene="ribA"
                     /locus_tag="ACHK3A_003447"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO8741490.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(12884..15559)
                     /gene="acnA"
                     /locus_tag="ACHK3A_003448"
     CDS             complement(12884..15559)
                     /gene="acnA"
                     /locus_tag="ACHK3A_003448"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO8741491.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGNITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDDVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(15709..15804)
                     /gene="ymiC"
                     /locus_tag="ACHK3A_003449"
     CDS             complement(15709..15804)
                     /gene="ymiC"
                     /locus_tag="ACHK3A_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO8741492.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(15918..16085)
                     /gene="yciX"
                     /locus_tag="ACHK3A_003450"
     CDS             complement(15918..16085)
                     /gene="yciX"
                     /locus_tag="ACHK3A_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO8741493.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQQKQ"
     gene            complement(16088..16228)
                     /gene="ymiA"
                     /locus_tag="ACHK3A_003451"
     CDS             complement(16088..16228)
                     /gene="ymiA"
                     /locus_tag="ACHK3A_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO8741494.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(16547..17521)
                     /gene="cysB"
                     /locus_tag="ACHK3A_003452"
     CDS             complement(16547..17521)
                     /gene="cysB"
                     /locus_tag="ACHK3A_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO8741495.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEIMFKDIKLPEK"
BASE COUNT         4462 a         4527 c         4197 g         4428 t
ORIGIN      
        1 attgtggaga ttctgtgaag atagttgaca atattaagtt ggggtcaatt catcagaaag
       61 acagcgactt gttgtcgcca gttctgtttt catcatcaaa tttatcggag ctaatacgaa
      121 tagtttcata gtcagattct ataagatatt tccccaacct cccccttgca ggcgtcagga
      181 gcagataacg caaaatgctc tgtaccgcag cttctctccg ctacagaaag cgcaactata
      241 gccacccaca gccaggttga ttataataac cgtttatctg ttcgtactgt ttactaaaac
      301 gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa agctatgggt
      361 tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactgtc catcgcctac
      421 ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta ccagaacgac
      481 aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat cgttctgcag
      541 tgcgatgttg ccgaagatac cagcatcgac accatgttcg ctgaactggg gaaagtttgg
      601 ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca gctggatggt
      661 gactatgtta acgccgttac tcgtgaaggc ttcaaaattg cccacgacat cagctcctac
      721 agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc tgccctgctg
      781 accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat gggtctggca
      841 aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc ggaaggtgtg
      901 cgtgttaacg ccatctctgc gggtccgatc cgtactctgg cggcttccgg tatcaaagac
      961 ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac cgttactatt
     1021 gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg tatctccggt
     1081 gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact cgaactgaaa
     1141 taatcgttct gttggtcaag acgggcggcg ttctgccgcc cgttatctct gttatacctt
     1201 tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt caggataacg
     1261 atttccttta cgaccaagga gcgcccatgg aaaaacgcca cattaccggc aaaagctact
     1321 ggtatcatga aacgcaatcc agtactgcgg agtatgacgt tctgcctctg gtcccggaag
     1381 ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagaa acgctggccc
     1441 ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggaattgttc cctgaccagc
     1501 ttaccgtgac ccgttcacag accttcaccg cttatgaacg cttgtcgacg gccctgacgg
     1561 ttgctcaggt ttgcggcgtc caacggttat gtaactacta ttcggcgcga cttacgccgc
     1621 tccccgggcc tgattcctcc agggaaagta atcatcggtt agcacaaatc acgcaatatg
     1681 cccgccaact ggctagctcg ccttctatta tcgacaaccg atcgcgccag catctgaatg
     1741 acgtcggcct aactgcctgg gactgtgtaa tcattaacca aatcattggt tttattggct
     1801 ttcaggcgcg gtcaattgcg acatttcagg cttatctcgg gcatccggta cgctggttac
     1861 ccggtctgga gatacaaaac tatgccgacg cgtcactgtt tgctgatgaa tcaatacgct
     1921 ggcgaagcag ctatgaggtg gaaaaactcc ctgaagagca cacaaaaagt tcaactgcag
     1981 aactttgcca actggccgaa acactctctc tccaccctat ttcactttcc cttctcgaaa
     2041 agttgctaaa cagcacacgg gtcaatacac agccggataa tcagcttgcg gcgttgttat
     2101 gcgcgcggat aaatggcagt cctgcttgtt ttgccacctg tatgaattca tcaaatgaat
     2161 ataaaaaaat cagcgccctt ctgcgcaagg gcgaaaatga aattaaccaa tgggctgacc
     2221 gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga gcacccgatc
     2281 gctttagcgc cgcccagttc agcccattac tcgaacacga aaaatcatca acgcagatta
     2341 ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg
     2401 gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca gccgctctaa
     2461 tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca gcttaaacag
     2521 caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt
     2581 ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca gatgaaaaaa
     2641 gtcatgcatg gcgaccgaat tatcgcggtt atccacagtg aaaaagaacg tgaatccgca
     2701 gagccagaag aactggttga accgtttctg actcgtttcg tgggtaaagt tcagggcaaa
     2761 aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat tccttgccgc
     2821 gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc
     2881 cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata catcactttt
     2941 ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct ggagaaagaa
     3001 gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga agatctgacc
     3061 gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga cgcccttttc
     3121 gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga tccaaccgcg
     3181 tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat
     3241 ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct ctgctcactg
     3301 cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc tgatggcacc
     3361 attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat
     3421 gacctggttt ctgactggct ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt
     3481 gctgaacaag tccgtttgtt agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac
     3541 cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga aaaaggtgaa
     3601 gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg
     3661 attgccgcta acatttgtgc ggcccgcgta ctgcgcgata agctcggttt tggcatctat
     3721 aacgtgcata tgggctttga tccggcgaat gccgacgcgt tggcagcgtt gctgaaaacg
     3781 cacggtctgc atgtcgatgc tgaagaagtg ctcacgctgg acggtttctg caaactgcgt
     3841 cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg cttccagtca
     3901 tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga ggcatacgcc
     3961 acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct gctgaaagcg
     4021 gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca aatggccgag
     4081 cgtcgccgtc tcaaccgaat ggcagaacgt gatgttggcg actggttata cgcacgcttc
     4141 ctgaaagaca aagccgggac cgacacccgt ttcgcggcgg aaattgtcga tatcagccgt
     4201 ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc ggcacctttc
     4261 ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt acaaattaaa
     4321 ggtgaaacgg cttacaaagt gactgacgtt attgacgtca ccattgccga agtccgcatg
     4381 gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ccattcctta
     4441 tgaatgggcc gtttatttcc ccgctctacc ttcatcatat cccggcagtt ttttaactga
     4501 ctttcgtttg aaaactggcg tttttccaaa actggattac aaataaatat ataaaaaaaa
     4561 caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta tttaaccgcg
     4621 gactccgctg ttaaccggag gatatgcatc atgaaaaccg ttagggagtc cacaacgttg
     4681 tacaactttc tcggatcgca caatccatac tggcggttaa cggaaagcag cgatgttttg
     4741 cgcttttcta ccaccgaaac cacagaacct gaacgtacgt tgcagttatc tgccgaacag
     4801 gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag tctgaccgtc
     4861 gatgaaagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg ggaatgggct
     4921 ggcaacgcgt ctgcatggca tgacacacca gcagttgctc gtgatttatc acacggactt
     4981 tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct cgacagccgg
     5041 gggaatatcc aacgcttcaa tcggttatgt gaagattaca cagggttgaa agaacatgac
     5101 gtcattgggc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc ggcatccagg
     5161 cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact gtggatacca
     5221 acacgtaaag gccagcggct gtttctgttt cgcaataaat ttgtccacag cggcagtggc
     5281 aagaacgaaa tttttttaat ctgttccggc accgacatta ccgaagagcg ccgcgctcag
     5341 gagcgactgc gtattctggc aaataccgac agtatcaccg gactgccgaa tcgtaacgca
     5401 atgcaggagt taatcgatca cgccattaat caggcagata acaataaagt tggggttgtg
     5461 tatcttgatt tggataattt taaaaaggtc aacgacgctt atgggcattt gtttggtgac
     5521 cagctattac gcgacgtgtc attggccatt ttaagctgtc tcgaacatga tcaggtgttg
     5581 gcgcgtccag gtggcgatga gtttctggta ctggcatcca atacctcaca aagcgcgctg
     5641 gaagcaatgg catcacgaat tttgacccgc ttacgactcc ccttccgcat tggtttaatt
     5701 gaagtttata ccagctgttc agtgggtatt tcactctctc ccgaacatgg ttcggacagc
     5761 gcggctatta ttcgtcacgc cgacacagca atgtacacag cgaaggaagg cggacgagga
     5821 caattttgcg tttttactcc agaaatgaat cagcgggtat ttgaatatct ctggctggat
     5881 accaacttgc gtaaagcact ggaaaacgat cagttggtta ttcactatca accgaaaatc
     5941 acctggcgtg gcgaagtgcg cagtctggaa gcactagtac gttggcagtc acctgaacgt
     6001 ggattgattc caccgttgga cttcatttcc tacgccgaag agtcagggct aattgtgcct
     6061 ttaggccgtt gggtgattct cgatgtagta cgccaggtgg caaagtggcg ggataaaggt
     6121 attaacctgc gagtggcggt aaacatttct gcacgtcagc tcgccgatca aaccattttc
     6181 accgccctga aacaggttct ccaggaactc aattttgaat actgccctat tgatgttgaa
     6241 ctgacggaga gctgcctgat tgagaatgat gagctggcac tgtctgttat tcaacaattt
     6301 agccaactag gtgcgcaagt gcatctggac gattttggta ccggttactc ttcactttcg
     6361 caactggcgc gctttccgat cgatgccatc aaacttgacc aggtttttgt tcgagatatt
     6421 cacaaacaac ctgtctcgca gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg
     6481 aatcttcagg tgatcgccga aggtgtagag agtgcaaagg aagatgcttt tttaaccaag
     6541 aacgggatca atgagcggca aggatttttg tttgccaaac cgatgcccgc cgtcgccttc
     6601 gaacgttggt ataaacgcta tctgaagcgc gcataagtag atgaatatat atacttagcg
     6661 atatattccg taattatata tcgtattccc ggatgataat tagccgttgc tgttaaacac
     6721 ttctctttta gcggcaattt ttgcattatt cattttccca cttttgctat aaggatgtcc
     6781 ttgatgtctg aatttgatgc ccagcgcgtt gccgaacgta ttgatattgt gttagatatc
     6841 cttgtggcag gcgattacca ctctgcgatc cataatctcg agatacttaa agcagaactc
     6901 ttgcgccagg ttgctgaatc tacgccagac atcccgaagg caccgtggga gatttaacaa
     6961 tcgtgccttc aggttttcct atttgaggat gcccggcgcg tcagtatttt tttatttagt
     7021 attataacgt tataagaatt acagcgatga attcccgaca acaaactatt ctacagatgg
     7081 tcattgacca gggtcaggtt agcgtaaccg atctggcaaa agccactgga gtttctgaag
     7141 ttaccattcg ccaggatctc aacaccctcg aaaaactgag ttacctccgc cgtgcacatg
     7201 gctttgcagt atcgcttgat agtgatgacg tcgaaacccg tatgatgagc aactatacgc
     7261 tgaagcgtga actcgccgag tttgccgcgt cactggttca accgggcgaa accatcttta
     7321 tcgaaaatgg cagcagcaat gccctgctcg cccggactct gggcgagcag aagaaaaatg
     7381 tcactatcat cacggtcagc agctacatcg cgcatttgct gaaagacgcg ccttgtgaaa
     7441 ttattttgct cggtggcgtg taccagaaaa aaagcgaaag tatggttggc ccgttgacac
     7501 gccagtgtat ccaacaggta catttcagca aagcatttat tggtattgat ggctggcaac
     7561 ctgaaactgg attcaccggt cgcgatatga tgcgtaccga tgtggtcaat gccgtgctgg
     7621 aaaaagagtg cgaagcgata gtcctgactg acagctcgaa atttggtgct gtacattcat
     7681 actccatcgg tcccgttgag cgattcaatc gcgtgattac cgattcgaaa atacgcgcca
     7741 gcgatctgat gcatcttgag cacagtaaac tcaccgttca cgttgttgac atttaaccat
     7801 ttcccttctc tacggatgat ttgcactttg gcaaatcatc cgctctaaga tgattcctgg
     7861 ttgataatta agactattta cctgttatta acactctcaa gatataaaat tattatcagc
     7921 gatataacag gaagtcatta tcacctgcgt gatataaccc tgcgcgcgag cggatttcac
     7981 ggaataattt caccaggctt attcttagct attatagtta tagagagctt acttccgtga
     8041 atcataaatt caggagagag tattatgttt gtaacgagca aaaaaatgac cgcggctgtt
     8101 ctggcaatta ccctggcaat gtctctgagt gcctgttcta actggtctaa acgggatcgc
     8161 aacaccgcaa tcggcgcggg tgcaggggca ttaggcggtg cagtactgac cgatggcagt
     8221 acgttgggta cattaggtgg cgcagctgtc ggtggtgtta ttggtcacca ggtcggtaaa
     8281 taatcgtaaa taaatgtcga ttatcgactc gcaattataa tacgcttgat tctgttcagt
     8341 acgaaataac tgaataatac acacagtaaa atattcgaaa gccacggata tatccgtggc
     8401 tttttatgtt aaccaccggc gagttttacc ttcatccctt tcgcttcaag cagtgatttt
     8461 aataaatcac gcttatcgcc ctggatttca ataattccat ctttaactgc cccgccgcag
     8521 ccgcattttt tcttcagttc cgctgccagt tttgtcagtt cggcatcatc gagatcgaca
     8581 ccggtaatca ggcaaacgcc cttaccttta cgtccactgg tctgacgctg aatacgcacc
     8641 acaccgtcgc ctttagggcg tacaggggcc gcttttggct catcaatacg tccggtttcc
     8701 gttgagtaga ccagacggct gttggaatca ctcatcatgc actccgctgt aaagaggcgt
     8761 tgatcgcttt cagcgtttgc gctggatcta ccgattgcgt taccgggcga ccaatcacca
     8821 tataatcaac accagccgcc aacgcctgtt ctggcgtcat aatgcggcgc tggtcaccag
     8881 cttcactccc ctgcggacga atgcccggcg taaccagttt gaactcctga ccaaatacct
     8941 gtttaaagcg cgcagcttcc tgagcagaac acaccacacc atcaaggcca catttttgcg
     9001 tcagtgccgc cagacgttct gcataatctg caggtgacag tgtcacgcca agatcggcca
     9061 ggtcgctggc ttccatgctg gtcaatactg tcacagcaat caaaagcggt gcatctttgc
     9121 caaacggcac cagtgcctca cgcgctgcgg tcatcatacg cgccccaccg gaggcgtgaa
     9181 cattcaccat ccacacgcct aagtcagctg cagcagcgac agcgtgcgct gcagtgttgg
     9241 gaatatcgtg aaatttcagg tcgaggaaga tatcaaaacc acgctgttga agttcgcgta
     9301 caaattgagg tccaaacagc gtaaacatct ctttgccgac tttcagacga caatcgcgtg
     9361 ggtcgatctt gtcgacaaag gccagcgcgg catcacgatt atgataatca agggcaacaa
     9421 ccacaggaga attcgtaaca gcgcgggaag aagatgaagc agttaacgtc atgaccagac
     9481 cttcttgatg atgggcaccg gaagtggcgc gaaacaggta aacggcgagc attctacccg
     9541 tggaaataaa aaattaacag ttgcgatttc tcccctggca ggcttttctg cgccggagtt
     9601 accggagcac aatgaacatc aattattagt atgttgtaac taaagtcgga tttttttaaa
     9661 aattacaggc catcaagacc gcgaatcggt ttaatggttg accaggcccg acaagacgga
     9721 caatgccagt agagggtgta tgcggtaaaa ccacatttct ggcagcgata acgaggctta
     9781 ctacgcacct tctcgccaac catgtcacgc agcaccatca gactctcttt ggcacgccct
     9841 tcttccgctt catttaagtg gtaatccatt agcttatgga acacacgcat ggtcggatga
     9901 cgctgaagct gacgcgtaat atagacctgt gcggcctcac taccgtcgcg cgcttcgata
     9961 atatccgcaa gcatcaattc agcatcggca ccggtgttct cttccaccgc gcgctgcagg
    10021 aattctgccc attcggcagt tttacccaac tgctgatagc aggtttgcaa catttccagc
    10081 gtttcgctga ccagttctct gtcctgagat atcacgcgtt gcagactttc gacggctttg
    10141 gcgtattctc ctttcgccat aaatacgcgt cccatcatga tggatacgcg ggcgctgttt
    10201 ttatctgccg ccgcaccttt ttttaacaag gccatggcgc gatcgagatc gtcgctggcc
    10261 atatgctgca gggctaactc acagtagaaa tgggcaattt cgacgcgctg cttatcttta
    10321 cccaacttca ccagacgttc ggcaacatca attgctttct gccactcgct ggtagcctgg
    10381 tagatttgta gcaactgttg cagcgcgcca atgcggaagt cagtttcatc ggtcagctga
    10441 ttgaacatgt cttccgcgcg gtcgtataac ccggcggcca tgtaatcacg ccccagttgt
    10501 tgaatcgcca acagacgctg ttcataggtc agcgaggcgc tttccattag ggtctgatgg
    10561 atgcgaatag cgcgatcaac ttcgccacgc gaacggaaca ggtttcctag cgtaaggtgg
    10621 gcttcaacgg tacccgtatc ctctttaagc atatcgagaa acaggtctac cgctttatcc
    10681 tgttgattac taagcaggaa gttaaccccc gctacgtaat cacgcgacaa gcggttggct
    10741 tcatcttgct tgttttgttg cgcacttctg cggcccatat accagccata ggcagcggct
    10801 acaggcaaaa gcagaaacaa caactccagc atagaaagtt attccttcgc cgctgacgaa
    10861 tgcggcgcta cagccacgtc agtcgcgggt gaaagctggt tttccagtcg ctttatttta
    10921 cgttcagcgc gcgccaggga aacacgaact cgcagccaga acagaccgca aatcagccaa
    10981 ccgatagcaa accccgcagc aaacaatacc gccagcaatg tggaaatacg gtactcccct
    11041 tgcgctaaca gataattaaa cgtcacctgt tgatcgttct gcgcacctaa cgtgaccgaa
    11101 atcacgaaga tcgctaacac cagtaagaaa atgagtaaat atttcacatt acttcccgtt
    11161 atgtggttag agcgaataaa ttatggtcaa cttaccgcaa tagcccttta aagtaccatt
    11221 ttcgcgccgt ctgcgaaatg gcatccacgc gcattgatga tgatttaagg gctaaattcg
    11281 gaaaatcaac cagcgttaac tttcttgttc tcgttgcgct atttcgcgat tttcttccgc
    11341 aggtggtgtt aatggcccac aaattcgttg cgcaagccac gttgccaccg ccaccagcgc
    11401 ccacgaaatc aacgtagcta ccaccagatc gcgtggccaa tgcatcccga gcagcaggcg
    11461 gcttcccatg actcccgttg cccagaccaa caagatagca atggttaacg ttcgccgacg
    11521 cggccacagc aaaccaacgg ccagcagcgc ccaactggca gcaaacatcg tgtgaccgga
    11581 aggaaaggca aaccccgtct ctttctgcca gtgtgaacgc aaatattgtg ggatattttt
    11641 ctcttcagcc aactgttctt tcaccagatt tccgcgttct gctcgcttta aagtgtagaa
    11701 ctcatcaacc ggaatatgat gtgttttttc cagccagata acaaaaggtc gtggttcctg
    11761 gactttgtct ttgatccagg atttaacgcc ttgtcccaca agaattgccg ccgccagaat
    11821 ggcaaataat acaaaggcag ccttaatgcg aaaacgcaga caccagagaa accagccgaa
    11881 taaaatcaaa tgtgtaatga cgccccaggg ctgggtgaca gtttcagtaa cccaaaaagc
    11941 cgcttttagt agccaacttt gttctccagg ttgccaacgc caaccagaaa tccatacggc
    12001 tactggcatg acaagcaata gtgcagctcc cactgcggta cgtctggcaa tcgaacgcat
    12061 ggcctctcct ttttgataag tcccacaatc ataactgaaa acgccagttc caggaaaaat
    12121 tgacagattt gtgccattcc gtgaacgatc gacgcgtcgt gattaggtga accccttctc
    12181 gttatggcaa aataagccaa tacagaacca gcattatctg gagaatttca tgcagcttaa
    12241 acgtgtggca gaagccaaac tgccaacccc atggggcgat ttcctgatgg tgggatttga
    12301 agaactggca accggacacg atcatgtcgc gctagtctat ggcgatatct ccgggcatac
    12361 cccggtactt gcgcgcgtcc attccgaatg tctgaccggt gacgccctgt tcagcttgcg
    12421 ctgcgattgt ggcttccagc tcgaagcggc actgacgcaa attgccgaag aaggccgtgg
    12481 tattttgctg tatcaccgtc aggaaggtcg taacattggt ctgctgaata aaatccgcgc
    12541 ttatgcactg caggatcaag gttacgatac tgtagaggct aaccaccagt taggcttcgc
    12601 cgctgatgag cgcgacttca ctctttgcgc tgatatgttc aaactccttg gcgtcaatga
    12661 agtccgcttg ttaaccaata acccgaaaaa agtcgaaatt ctgaccgaag cagggattaa
    12721 tattgttgaa cgcgtaccat tgattgtagg tcgtaacccc aataacgaac attatctcga
    12781 taccaaagcc gagaaaatgg gccatttgct gaacaaataa ccctcttgca ttgtgtaatt
    12841 catttgcttg ccggaagcaa aataaccggc aagcaaatac ttgttacttc aacatattac
    12901 gaatgacata atgcaaaatg ccgtcgttct ggtagtaggt caactccgtc gcggtgtcga
    12961 tacgacaacg gcagggtacg acttcctggc taccatccgc gcgcgtaagc gtcaccggaa
    13021 ccgtcgcgcc gggttgtagg ttttgcagat cgccaatatc aatcttctct tccccggtta
    13081 gccctaacgt tttacgcgtt acgccttgcg gaaattccag cggcaggatg cccatgccaa
    13141 ttaaattcga acggtgaatt cgttcaaacg attcggcaat caccacacga ataccaagca
    13201 gacgcggacc ttttgccgcc cagtcacggc tggagcctga tccatactct ttcccggcaa
    13261 tcaccgccag cggcgtttgc tcctgcttat agcgcatcgc ggcatcatag atagagacat
    13321 catcgctgtc aggtaaatgc cgcgtcatcc ccccttcaac gccaggcacc atttcattac
    13381 ggatgcgaat attggcgaag gtgccgcgca tcatcacttc atggttacca cgccgcgaac
    13441 cgtaggagtt aaagtctttt cgctcaacac cccgaccttg tagatatcga cccgccgggc
    13501 tgtcgggctt aatactgccc gccggagaga tatggtcagt ggtgactgaa tcccccagca
    13561 ttgcgaggat ccgcgcaccg tgaatatctt ccacaggtgc tggtgttgcc tgcatttcat
    13621 caaagaatgg cgataagcga atataggttg aatcttcctg ccaaccgtag gtatcggatc
    13681 gtgtgacgtt aatttccttc cactctgctg tgccttcaaa aacttctgcg tactctttgc
    13741 ggaacatttc tgtggagact tgttctaccg cacgggcaat ttcttgtgcc gagggccaga
    13801 tatctttcag atataccggg tcgcctttac gatcgtgacc aataggctca gaagcgaggt
    13861 taatattcat atttcccgcc agcgcatagg caaccaccag cggcggcgag gccagccagt
    13921 tagttttaac cagcggatgg atacggcctt caaagttacg gttgccggac agcaccgcac
    13981 cgacggttaa atcgcctttt ttgattgccg tttcgatagg atcgggcagc ggcccagagt
    14041 taccaataca ggtggtacaa ccgtacccca caaggttaaa ccccagttcg tcgagatacg
    14101 gtgtcaattt cgcttttgcc agatagtcag aaacaacttt cgagcctggt gccagcgacg
    14161 ctttgaccca cggttgccgc ttgaggccca gagttacagc ttttttcgcc agcaagcctg
    14221 cggccatcag cacacttggg ttagaggtgt tggtgcacga ggttatcgca gcaatgacca
    14281 ccgcgccatc aggtaactga tactgatgtc cgttcataac ataatcgacc ggctggcgat
    14341 ctttatgcgt ggcattcact tccagttcgt tactggcggc aaatgctttt ggtacatcgg
    14401 gcagtgcaac gcgatcctgt gggcgtttag gccctgccag gctcgcttca acgtcattca
    14461 tatccagttc taacgtactg gtaaaaattg gttcatcgcc cgggttacgc cacatgccct
    14521 gcgctttggc atatttttcg accaactcga cctgatcttc gctgcgcccg cttaaacgca
    14581 tgtaatcgag ggtgacagca tcgattggga agaagccaca ggtggcacca tattctggcg
    14641 acatattggc aatggtggcg cgatccgcca gcggtagtga atccagccca tcaccataaa
    14701 attcgacgaa tttccccacc acgccatgtt tgcgcagcat ttgggtaacg gtgagaacca
    14761 ggtctgtggc ggtaatacct tcacgtaatt ttccggtgag tttaaagccc actacgtccg
    14821 ggataagcat ggaaaccggc tggcctaaca ttgcggcttc ggcttcgatg ccaccaacgc
    14881 cccaccccag cacgccaagg ccgttgatca tggtggtgtg cgagtcagta ccaacgagtg
    14941 tatccggata agcaatccat tcaccgtcct gcaattcgct ccacactgct ttgccgagat
    15001 attcgaggtt aacctgatgg caaatgcctg tgcctggcgg cacgacgcta aaacgactga
    15061 acgcctgctt tccccatttc aggaacacat aacgttcgtg gttgcgctcc atttccaggc
    15121 gtacgttttc ttcaaatgcc tcatcatcac caaaacgatc gacggtcacc gagtggtcaa
    15181 tgaccaggtc gaccggtgag agcgggttaa cctttgcagt atcgccaccg aggcgtttaa
    15241 ccgcttcgcg cattgccgcc agatcaacaa cggcaggtac gccggtaaag tcctgcatca
    15301 gcacccttgc cgggcggtag gcaatttcac ggtcagcatg ggcatttttc agccatcccg
    15361 ccagcgcgtg gatatcctct tcggtaaccg agttaccatc ctgccagcgc agcaggtttt
    15421 cgagcaaaac tttgagtgac ttaggcagac gggtgatatt gcccagtgat ttggcagcaa
    15481 gcggcaggct gtagtagtgg taagttttat ctttggcctg caacgtgtcc ttactggctt
    15541 ctcgtagggt tgacgacata gctcctcctt aatgacaggg ttgcgaagcc ctgattctct
    15601 tcagggtgaa tattaaagat aacacaaaca tcgcgtaacg atttgataac aacccaaatt
    15661 gataaaagag gagaaacctt gagacaaagc aaacagcggg gtcagggatc agtgagtaat
    15721 gatccagagg agttcggccc agaagagcat cgacagagaa aatgcgccca tccaggacca
    15781 atatttcata tttgtgttga tcataggcta cacctttaac ggttattact ttgtgttgag
    15841 ataactcaac ctgttattgc tgcggtgtat taaaagccct ttaattcatt aaaaatgggt
    15901 aataagaatg atgataatta ttgtttctgc tgatcatctt cagcaaacag ttctataaag
    15961 gcttgctgct gcgatgttag ttgccagtcg tctggaataa cgacggaaca ttcccgtttc
    16021 gtttctgttt cattgtcact atgctgacat aatggtgacg actccagttg ctcctgaccg
    16081 accatagtta tccccacact ttccaaatca gcagtgcgac aaccacccag aaaagagcag
    16141 aaccaagaaa taccgccagc caggctttgc gttttaattc agggtcccgg cggggttcct
    16201 gatttccaga aggcattgct aacctcatat aatcgccatc acttatcagc aagacgttga
    16261 acgattggtg taattaatca tacgatgaga caaatcctaa agaaacttta gtcaaaaatc
    16321 cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga aatagatatt
    16381 tgaaaactcg agtttgcgag gcgggtaatt agacactact ttacaggtaa tttaccctat
    16441 aaatatgagg gataaaaata ttgtgcacgg tatacaaata agcaacaaaa gagaatatat
    16501 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
    16561 gttttatatc tttaaacatg atctcaattt cttcattaga gcgtaatgcg acagccgcat
    16621 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
    16681 gcaaaaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
    16741 catcaacgcg caccaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
    16801 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
    16861 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagctctgaa cgtccggtaa
    16921 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
    16981 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
    17041 acatcactag atcttcatac agatgcaacg cttctgtggc gatagcgaaa tcagcattgc
    17101 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
    17161 ggggataacg ctcaataaag cctttgatga cgtttggtaa cgcgtagcgt gcctgggtat
    17221 gcgtggtggc gatatacagc gagcctttat ccggccaggt gtgctctccg gcgaccgatt
    17281 ttatggcatc aactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
    17341 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
    17401 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
    17461 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
    17521 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
    17581 actaatacga tttaataata aaaaaagtgc taac
//
LOCUS       DAZGHF010000099        17491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGHF010000099 DAZGHF010000000
VERSION     DAZGHF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.099"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..641
                     /locus_tag="ACHK3A_003453"
     CDS             <1..641
                     /locus_tag="ACHK3A_003453"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO8741496.1"
                     /translation="GLADSRTRGLADSRTRGLISYELTPQEYNAIELLNSNNLMWGTY
                     NLKKLFGPAIRGKRLKNADRIPGSLPFVTAGEGNEGISAFISNDVVVFPENTITIDMF
                     GSAKYRNYEYGADDHVAVVHTEFFDRNAVIFITASIHKSSCTGKFNYGRNFYAKDADE
                     LMVSLPSKDNNPDYSFMETLILAVHKLVIKDVVQYTDKKLVAYRQITSEKVV"
     gene            993..1118
                     /locus_tag="ACHK3A_003454"
                     /pseudo
     CDS             993..1118
                     /locus_tag="ACHK3A_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            1159..1234
                     /locus_tag="ACHK3A_003455"
     tRNA            1159..1234
                     /locus_tag="ACHK3A_003455"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1192..1194,aa:Ile,seq:cat)
     gene            1242..1318
                     /locus_tag="ACHK3A_003456"
     tRNA            1242..1318
                     /locus_tag="ACHK3A_003456"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1276..1278,aa:Arg,seq:tcg)
     gene            1332..1408
                     /locus_tag="ACHK3A_003457"
     tRNA            1332..1408
                     /locus_tag="ACHK3A_003457"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1366..1368,aa:Arg,seq:tct)
     gene            1500..2459
                     /gene="stxA2b"
                     /locus_tag="ACHK3A_003458"
     CDS             1500..2459
                     /gene="stxA2b"
                     /locus_tag="ACHK3A_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO8741497.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            2472..2735
                     /gene="stxB2b"
                     /locus_tag="ACHK3A_003459"
     CDS             2472..2735
                     /gene="stxB2b"
                     /locus_tag="ACHK3A_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO8741498.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            3241..5151
                     /locus_tag="ACHK3A_003460"
     CDS             3241..5151
                     /locus_tag="ACHK3A_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO8741499.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGC
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFNGTPANHAARFTEVVDQYRADLAD
                     MAGQCVGGSADSVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPDIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAIL
                     SCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLVD
                     DSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKY
                     AFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWHT
                     LAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDT
                     VEVKANKAGAAA"
     gene            5291..5473
                     /locus_tag="ACHK3A_003461"
     CDS             5291..5473
                     /locus_tag="ACHK3A_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8741500.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            5499..5771
                     /locus_tag="ACHK3A_003462"
     CDS             5499..5771
                     /locus_tag="ACHK3A_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO8741501.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            5848..6063
                     /locus_tag="ACHK3A_003463"
     CDS             5848..6063
                     /locus_tag="ACHK3A_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8741502.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            6068..6601
                     /gene="rrrQ"
                     /locus_tag="ACHK3A_003464"
     CDS             6068..6601
                     /gene="rrrQ"
                     /locus_tag="ACHK3A_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8741503.1"
                     /translation="MSRKFRYGLSAVVLAMIAAGASAPEILDQFLDEKEGNRTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCAQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCLPSTFYKRLNAGDRKGACESIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            6598..7065
                     /locus_tag="ACHK3A_003465"
     CDS             6598..7065
                     /locus_tag="ACHK3A_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO8741504.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPRLADTVERDYFTLRERLMMMQKQLEGAQEYIRTQCLK"
     gene            7368..8174
                     /locus_tag="ACHK3A_003466"
     CDS             7368..8174
                     /locus_tag="ACHK3A_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO8741505.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            8155..9861
                     /locus_tag="ACHK3A_003467"
     CDS             8155..9861
                     /locus_tag="ACHK3A_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO8741506.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            9861..12005
                     /locus_tag="ACHK3A_003468"
     CDS             9861..12005
                     /locus_tag="ACHK3A_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO8741507.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            12163..13170
                     /locus_tag="ACHK3A_003469"
     CDS             12163..13170
                     /locus_tag="ACHK3A_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741508.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            13194..14408
                     /locus_tag="ACHK3A_003470"
     CDS             13194..14408
                     /locus_tag="ACHK3A_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO8741509.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            14464..14853
                     /locus_tag="ACHK3A_003471"
     CDS             14464..14853
                     /locus_tag="ACHK3A_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741510.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            14903..15364
                     /locus_tag="ACHK3A_003472"
     CDS             14903..15364
                     /locus_tag="ACHK3A_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741511.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQESVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            15348..15911
                     /locus_tag="ACHK3A_003473"
     CDS             15348..15911
                     /locus_tag="ACHK3A_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741512.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            15911..16561
                     /locus_tag="ACHK3A_003474"
     CDS             15911..16561
                     /locus_tag="ACHK3A_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO8741513.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            16558..>17491
                     /locus_tag="ACHK3A_003475"
     CDS             16558..>17491
                     /locus_tag="ACHK3A_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO8741514.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKASEAAEAATRAITAAGNAEESSTRAGESEKAAGADAEKARLHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGL"
BASE COUNT         4548 a         3989 c         4866 g         4088 t
ORIGIN      
        1 gcggactcgc ggactcgcgg actcgcggac tcgcggactc gcggactcgc ggactgataa
       61 gctatgagct tacaccacag gaatataacg cgattgagct attaaatagc aataatctta
      121 tgtggggcac ttataacctc aaaaagttat ttggccccgc aataagaggt aagcgtctga
      181 aaaacgcaga tcgtataccc ggctccttgc cttttgttac tgcgggtgaa ggcaatgaag
      241 gtatttctgc atttatcagt aatgatgttg ttgtattccc tgaaaatacc attacgatag
      301 atatgtttgg ctccgcaaaa tacagaaatt atgagtatgg tgcagatgac catgtggcag
      361 ttgttcatac agaattcttt gacagaaatg ccgttatttt cataacagca tcaattcata
      421 agtcttcttg taccggaaag ttcaactacg ggagaaattt ctatgcaaaa gatgccgacg
      481 agttaatggt atctttgccg tcaaaagata ataaccctga ttattctttc atggagacgc
      541 taattctagc agtacataaa ctggtcatca aagatgttgt ccagtacact gataaaaagc
      601 tggtagcgta caggcagatt acatctgaga aggttgttta gtgcaaatcc ccacctgaca
      661 gggtggggaa tggtcaggtt atcgtgccct gatgcagaaa aacagtttac aatcgtaaaa
      721 atcatcatat catgacaaaa gtggttacat tgccacgctg cttaacccgc cgatgcgcgg
      781 gttttttgta tccagaatcc tgtaagttat acggaaagta cacagaaagg aaggtgcgac
      841 cgcaattaat aacaaaatct taaaaatcgc atatggcact attagttttc taaatattgt
      901 atattttaag tattgcagga taaccctgta acgaagtttg cgtaacagca ttttgctcta
      961 cgagtttgcc agcctccccc agtggctggc ttttttatgt ccgtagcgtc aaagcagcaa
     1021 tggcgctggg gcgtcgtgca actggcgttg agctggagac tgaacgtttt gagcagacgg
     1081 tcagggaagt tcaggattta gccagtcaga acggatgata ttgcaggatt agttacgtac
     1141 cgttattatc ctgcgcccgg ccctttagct cagtggtgag agcgagcgac tcataatcgc
     1201 caggtcgctg gttcaaatcc agcaagggcc accatcacat accgccatta gctcatcggg
     1261 acagagcgcc agccttcgaa gctggttgcg cggggttcga gtcctcgatg gcggtccatt
     1321 atctgtacac tgcgttgtta gctcagccgg acagagcaat tgccttctaa gcaatcggtc
     1381 actggttcga atccagtaca acgcgccata cttatttttt ctggctcgct tttgcgggcc
     1441 ttttttatat ctgcgccggg tctggtgctg attacttcag ccaaaaggaa tacctgtata
     1501 tgaagtgtat attgttaaaa tgggtactgt gcctgttact gggcttttct tcggtatcct
     1561 attcccggga atttacgata gacttttcga cccaacaaag ttatgtctct tcgttaaata
     1621 gtatacggac agaaatatcg accccacttg aacatatatc tcaggggacc acatcggtgt
     1681 ctgttattaa ccacacccca ccgggcagtt attttgctgt ggatatacga gggcttgatg
     1741 tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat ttatatgtgg
     1801 ccggattcgt taatacggca acaaatactt tctacaggtt ttcagatttt gcacatatat
     1861 cagtgcccgg tgtgacaact gtttccatga caacggacag cagttatacc actctgcagc
     1921 gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg gtttcatcat
     1981 atctggcgtt aatggagttt agtggaaatg ccatgaccag agatgcatcc agagcagttc
     2041 tgcgttttgt cactgtcaca gcagaagcct tacggttcag gcaaatacag agagaatttc
     2101 gtctggcact gtctgaaact gctcctgttt atacgatgac accggaagaa gtggacctca
     2161 cactgaactg ggggagaatc agcaatgtgc ttccggagtt tcggggagag ggtggtgtca
     2221 gagtggggcg aatatccttt aataatatat cagcgatact gggcacagtg gcggttatac
     2281 tgaattgcca tcatcagggg gcgcgttccg ttcgcgccgt gaatgaagag atacaaccag
     2341 aatgtcagat aactggcgac aggccagtta taaggataaa caatacgtta tgggaaagta
     2401 ataccgcagc tgcttttctg aatcgcaggg ctcactcttt aaatacatcc ggagaataac
     2461 aggagttaaa tatgaagaag atatttgtag cggctttatt tgcttttgtt tctgttaatg
     2521 caatggcagc tgattgtgca aaaggtaaaa ttgagttctc taagtataat gagaatgata
     2581 cattcacagt aaaagtggcc gggaaagagt actggactaa ccgctggaat ctgcaaccgc
     2641 tactgcaaag cgcacagtta acaggaatga cggtaacaat caaatcaaat acctgtgcgt
     2701 caggttcagg atttgctgaa gtgcagttta attaatatca gaagcattgc tggtttcgtg
     2761 gtgtgcagca atgtagttac agtgtaatca atgtcacaat tcagtcagtt gaaggttgtc
     2821 tgcccgactg agaatttgtt aaaaaaatcc tgcatggtga atccccctga gcggaggggc
     2881 atatcagcgt cacaggtgtt tctgttttac ctctatcctt tctgtgcggg ttcaggtgct
     2941 gatactgaac tcaccgggag gcacccggca ccatgcatga acggtacata gcgcatacac
     3001 cagcccctct ccggaggggc tttcttgtgg gcaaaaaaaa gcccgagtgg gttcgggcaa
     3061 tagcatgaga tatacatttt tataatcgaa tggattttaa ccaggattca taaggctgcg
     3121 caactgcgcg gcctttttcg tatttcgggc tgtagtcttc cttctgccat tgtcctgtaa
     3181 cttccggact tcagcccgcc ccttatctga ctcacaacat tatcccggcc gggaggattc
     3241 atggcattta aacactatga cgtggtcagg gcggcgtcgc cgtcagacct tgcggaacga
     3301 ataactcaaa aactgaagga agggtggcag ccttatggta gtgcgctgat ttcgacagct
     3361 ggttatggtg cggagttcat ccagccagtt gtgagtgagg ggagcatctc atcaccagag
     3421 gagccaggca accgtccgac gacctcagcg ccttctgttg cgccagaata ttactatgtg
     3481 atcgcgcttg ctggtcagtc caatggtatg tcatacggtg agggactgcc attaccggat
     3541 acattcgaca gccctgatcc acgtattaaa cagttagcgc gtcgcagtac ggtgacaccg
     3601 ggaggtgctg catgtatcta taacgatatt atcccggcag atcactgtct ccatgatgtg
     3661 gaggatatga cagggcttaa tcatcccaaa gcggatttat cgaaaggtca gtacggctgt
     3721 gtgggacagg ggctgcatat cgccaaaaag cttctgccat ttataccggc gaatgcgggc
     3781 attcttcttg ttccgtgctg ccgtggtggt tcagctttca ccaccggaac cgatggcaca
     3841 tacagtgaca ctactggcgc ctcagagagt tccacccgct ggggggtggg caggccgctg
     3901 tataaggacc tcattggtcg tacaaaagcc gcgctggcga agaatccgaa aaatgtgctg
     3961 cttgccgtgg tatggatgca aggggaattt gattttaacg gaacgccagc gaatcatgca
     4021 gcccgtttta cagaagtggt ggatcaatac cgtgcggacc ttgcagatat ggcggggcag
     4081 tgtgtgggtg gctcagctga cagtgttccc tggatttgtg gagacacaac atatttctgg
     4141 aagcagaaga acgaaacggc gtaccagacg gtgtatggta gttacaaaaa taaaacggaa
     4201 aagaatatcc atttcgtgcc gttcatgacc gatgagaacg gggtgaatgt gccgacgaac
     4261 aaaccggaag aagacccgga tattccggat atcgggtatt acggttcaaa atggcgaacg
     4321 gacagaagca cctggacatc tcaggacagg gccagtcatt tcagttcatg ggctcgccgt
     4381 gggattattt ccgaccgtct ggcaacggcg attctgagct gcgcgggtaa gtcttctgcg
     4441 tttgttaatg gtactgccgg ggtggttgtt ccagacagac cggttaccac ctcagagtct
     4501 gtaatttttt acgatgccaa aaaagcttca gacaatcagc tgaaacctta tggctgggac
     4561 ggtatggatg gcagacgcac actggttgat gacagcggca ataaagctct gcgaattgag
     4621 aaaaataaca gcgcgaaatc ctggtcaatg tactgtgata ttgctgcaga caaggcaaaa
     4681 cttttactgg aaaaaggcgg ggaaattgct gtccggttta aaatccccga aaacgtcaat
     4741 cttgagacaa ccagaaacaa gtatgccttt ggtttgtact ggcgaatagc ggaatggccg
     4801 ggtgagggtg gtgaaggcta tctgagttct ttctttgtcc agacagataa agccagtatt
     4861 gatgttgcat accatcatac agttaatcaa caaaaagaac ttggcacgtt tggcgcattc
     4921 gaccatgact ggcatacgct tgcatttaaa tttaagggca gtaacagcat taatgttact
     4981 ccggtgcttg atggtgtgga tggacaggcg tttgacctgg tgaaatgggc caatactgct
     5041 aatggactca acaggtttgt cattacggat attacaggta gtgcagaaac ctaccctgta
     5101 cttattgata cggtggaagt taaagcaaac aaagctggag cagccgcata attgctaaaa
     5161 aaagccgcca gcggcaggaa tggaagctgg cggaggtaat cccaatggag aatgtaaaga
     5221 aaagatgctt tcgtacattg gttttttaaa tgaaaacagt tctcattgtc aaccataacg
     5281 gtaagaaatt atgatattta ttcatcaggt gatgctgtac ttctgtacgg cggtctgtgt
     5341 gctgtatctt ctttcgggtg ggtacagggc tgtgcgcgat ttctggtgca ggcagattga
     5401 taaaagggcc gctgagaaaa tcagcgccag tcagtcagcc ggaagcaaac ccgaagagcc
     5461 cgttactcct taacaacccc tttcagcgag aaaatcccat gtcagaaatc acatccctgg
     5521 tcactgcaga ggcagtgaag gaagtcctgc gctctgaaga agtccggagc gcactgaaac
     5581 agaaacttcg ccagaatctt gaggcgcgtc ttgatgcaga agttgatgcc attctggatg
     5641 agctgcttgg tgtacaggca gagccaccga ctgaagcggg agataccacc gcagagagcg
     5701 gtgaagttca gcctgaatca ccggtcgccg atgcgactga acctcaaccc gaatcggtca
     5761 tgatgctgta acgaggagtc agggccatca gtaaacggct gctggccttt ttcatgttgt
     5821 gagcttccgg attgcgggag acggggtatg taccagatgg aaaaaatcac aacaggtgtg
     5881 tcatacacca cgtcagcggt gggaacgggc tactggttcc tgcagttgct ggacagggtt
     5941 tccccgtctc agtgggcggc aataggcgtg ctggggagtc tgctgtttgg gctgctgaca
     6001 tatctgacga acctgtattt caaaattaaa gaagaccggc gtaaggcggc gcggggagag
     6061 taagctgatg agcaggaaat tccgctatgg tttatcagcg gtcgttctgg cgatgattgc
     6121 tgcaggtgct tctgcgcctg aaatcctcga ccagtttctt gacgaaaaag aaggtaatcg
     6181 caccacagca taccgtgatg gtgcgggtat ctggaccatc tgccgtggtg ccacccgggt
     6241 ggatggtaag cccgtcgttc ccggcatgaa gctgtcgaag gaaaaatgcg cacaggttaa
     6301 tgccattgag cgtgataagg cgctggcatg ggtggagaaa aacatcaaag tgccactgac
     6361 cgaaccccag aaagcgggta ttgcgtcatt ctgcccgtat aacattggcc ccggtaagtg
     6421 tctcccgtcg acgttttata agcggcttaa tgccggtgat cgtaaaggtg cctgtgagtc
     6481 gattcgctgg tggattaagg acggtgggcg tgattgccgc acacgttcaa ataactgcta
     6541 cggacaggtt attcgtcgtg accaggaaag cgcattagct tgttggggga tagatcagtg
     6601 agaagagtaa ccgcgattat ttacgctctg gttatctgca tcatcgtctg tctgttatgg
     6661 gctgttaatc attaccgcga taacgccatc acctacaaag cgcagcgcga taaaaaagcc
     6721 agagagctgg agctggcaaa cgcaaccatt actgacatgc agcagcgcca gcgtgatgtt
     6781 gctgcgcttg atgccagata ctcaagggaa ttagccgatg cgagagctga aaatgaaact
     6841 ctgcgcgctg atgttgccgc tggtcgtaag cgcctgcgga tcaacgccac ctgtccaggc
     6901 tccgtgcgtg aagcccccac cacctccggc gtggataatg caaccggccc ccgactggca
     6961 gacacagttg aacgggatta tttcaccctc agagagcggt tgatgatgat gcagaagcag
     7021 ctggaagggg cacaggaata tatccgcact cagtgcctga aataagtttt gctgatgcgc
     7081 cgtatcgtcg ccgtattccc gtattaacag cgactgcagc cctgacgggt acctcctgcg
     7141 tgagtgtgcg gggataatca aaaacgatac acaccgggtt tccctcattt ttcaggagac
     7201 gtgagcattt acccgcgaag ccgcctgtcc ggtgcggtgg tggaagaaac cggataaaac
     7261 aacctcattg tacaaatatc gatcaagtat ggcgctgctg tgtgaattct gaaaaatcac
     7321 agcggtcatt atgcgtcagt ttttaacaga ggacgtcaga aagtgacatg gcaaagctgg
     7381 actggaaaaa gctggagcag gcattccgac gcgaacatgc cgaaacggga ataacattac
     7441 tggactggtg ccggaagaaa aagattaatt acaacaccgc cagaacccgt ataaaaatgg
     7501 gcaaaatcga tcatgaaatt gatcataaaa ccgatcatga aatcgatcat gacatctcag
     7561 atgaagaacc cagcaatgac gcgtgttccg acgatgaaaa atgcgcaaaa aactctgaaa
     7621 aaaactgcgc aaattcggca gaaacgaaac ggattcgtgg ttcccgactt ttacctcctt
     7681 caaacgcttt ttctcagcga aacacccacg ccgtaagaca ccgtggatat gcgaagtatc
     7741 ttgaggcaga taacctcatg gatgatgcgt ccgacatggt gctgttcgat gaactggtgt
     7801 tcacccgggc ccgcgcactt tcagtaacta aggcacttaa agggatgttc gccgacctgg
     7861 aagaggcaac tgacgtggaa acccgcgttg ctctttacga caaaatactc aaagctgaac
     7921 aggcccttga ccggaatatt gcccgtatcg agtcaattga acgctcattg ctgacgctgg
     7981 acgtcctggc tgagacagca ccaaaacttc gtgctgaccg ggaaagaatc aacgccgcca
     8041 gagataaact cagagcggaa accgatattc tgaccagcca gcgccggggg gttattacgc
     8101 cagtcagtga catcgtgtca tcgctgcatg aaatgagtaa ttcggggaga ctggatgaca
     8161 ttccggaaga ataaaacgcg atgtgatgaa cctgcagaaa tgactgagac cgaacaacgt
     8221 ctgttcatta tgacaaaact gagcaatccc tggtggcgcc tcaatcatct ctacaaaata
     8281 cagaacgaaa aaggtgaact ggtcaccttc agaatgcgac cggcgcaacg tcagttgttt
     8341 cgtaacatgc ataataaaaa cattatcctg aaagcgcgcc agctgggatt ttccacagcc
     8401 attgatattt atcttctcga ccaggcatta ttcattccgc atctcaaatg cgggatcgtc
     8461 gctcaggata aacaggctgc cagtgaaatt ttccgcacaa aaattgctgt accgtttgat
     8521 catctccctg actggctgag agcctcattc accatcgttg aacgtcgtag cggtgccagc
     8581 ggtggctata tcctgtttgg tcacggctcg agtattcagg tggcaacctc atttcgctca
     8641 ggtacggtgc agcgcctgca tatctcagag cacggcaaaa tttgcgcgaa atatccggct
     8701 aaggcgaaag agctgcgaac cggtacgctt aatgccgtct ctgatgaatg cattattttt
     8761 gatgaatcca cggctgaagg cgtgggtggt gatttttacg agatgagtaa ccgtgcacag
     8821 gagagcactg catcaggctt attgctgacg gcacaggatt ataaattcca tttttacgca
     8881 tggtggcagg atcctaaata cagcgccaga gtgcctgaaa gcgggctgaa gctgtcacgg
     8941 gaaaaaatga cgtatttttc tgcggttgag aaggcaatga acatcacgct taccgatgag
     9001 cagaagcagt ggtacatcag taaggaaact gaacagcgtg aggaaatgaa gcaggagttt
     9061 ccctcaacgc cacaggaggc gtttctgacg tccggacgac gtgtgttcag tgccgaaagt
     9121 acattgcagg cagaatcatt ctgttcgcca ccgatgattg tttatgacat tgaacctgtt
     9181 acaggagcga agactaaagc tcagtctctg cgtgaaggaa ataaaaacga gttgcagcgg
     9241 acgctgatga attatctgct ggtatgggaa ctgccggatc cggatgaaga gtatgtttgt
     9301 ggggcagata ctgccgaagg gctggagcac ggagaccgct catcgctgga tgttgtcaaa
     9361 cgcagtaatg gcgagcaggt ggctcactgg ttcgggcatc tcgatgctga actttttgct
     9421 catctcattt cgcaggtctg tcgtatgtat aacaacgcgt ttgtggggcc ggagcgtaat
     9481 aatcacggac atgcagttat cctgaaactc cgggaactct atccgacacg ttatatctac
     9541 aacgaacagc atcttgacca ggcatatgac gacgatacgc cccgccttgg ctggctgaca
     9601 acccgtcaga gcaaacctgt tctgaccgaa ggaatgaaaa cgctcctgaa taatggaata
     9661 tcagggatcc gctggtcagg cacattatcg gaaatgaaca cctacgttta tgacgcgaaa
     9721 ggctccatga atgcacagga aggctgcttt gatgatcagc tcatgagcta catgattgcc
     9781 caggagatgc gcgccagaat gccggtgagg gtaaaacaga aaacggataa acgcagaacc
     9841 acacactgga tggcacactg atgaaaaatg aaattaacac cacagcgatg aaaaacgatc
     9901 atggatccac gccgcgtttt tctcagcgtc agttactgtc tctctgttct gatattgaca
     9961 gtcagcctct ctggcgtgat gccgcaaaca aggcctgtgc gtattatgat ggcgaccagc
    10021 tggcaccgga agttatccag gtactgaaag atcgcggtca gcccatgacc atccataacc
    10081 tcattgcccc cacggtagat ggtgtactgg gaatggaggc aaaaacaaga acggacctga
    10141 tagtgatgtc agacgatccg aacgatgaaa cagagaaact ggcagaggcc attaatgcgg
    10201 agtttgctga tgcgtgccgt cttggcaaca tgaataaggc ccgctccgat gcctatgcgg
    10261 aacaaatcaa ggcggggctc agttgggtgg aggtcaggcg caacagcgat ccgttcggac
    10321 ctgaattcag ggtatccaca gtcagcagaa atgaggtttt ctgggactgg ctcagccggg
    10381 aatccgacct gagtgactgc cggtggctga tgcgtcgccg ctggatggat accgatgagg
    10441 caaaagccac gttcccggga atggctcagg ttatcgatta tgctattgat gactggcgtg
    10501 gttttgtcga taccacgatt actgaaggcc agcccagtcc gttgatgagt gcatgggaag
    10561 agtatcagtc atgggatcgc agggagaatg aatggcttca gcgtgaacgc cgtcgtgtgc
    10621 tgcttcaggt ggtttattac cgtacattcg aacgtcttcc ggtgattgaa ctcagtaatg
    10681 gacgggtggt ggcttttgat aaaaataatc tgatgcaggc ggtggctgtg gcatccgggc
    10741 gggttcaggt gaaagtcggg cgggtaagtc gtattcgtga agcctggttt gtgggcccac
    10801 actttattgt ggatcgcccc tgtagtgctc cgcaggggat gtttccgctg gttcctttct
    10861 ggggataccg aaaggataaa accggggagc catacgggtt aatttcccgc gccattccgg
    10921 cacaggatga ggtgaatttt cgtcgtatca agctgacctg gctgcttcag gccaaacgcg
    10981 tgattatgga cgaggatgcc acccagttgt cagacagcga cctgatggag cagatcgaac
    11041 gtccggatgg cattattaaa ctgaatccgg cccgaaaaaa tcagaaaagt gtcgcagatg
    11101 tttttcgggt tgagcaggat tttcaggttg ccagccagca gtttcaggtc atgcaggaat
    11161 cggaaaaact tatccaggat accatgggag tgtattccgc atttctcggg caggattcag
    11221 gtgcgacgtc aggcgtggct atcagtaacc tggtggagca gggggccaca actcttgcgg
    11281 aaatcaacga taactaccag tttgcctgcc agcaggtggg aagactgttg ctggcttatc
    11341 ttctcgatga cctgaaaaaa cgccgtaatc atgcagtggt gattaatcgc gatgatcgcc
    11401 agcgtcgcca gaccattgtc ctcaatgctg aaggtgataa tggtgaactg accaatgata
    11461 tttcaaggtt aaatacacat attgcgctgg cgcctgttca gcagacaccg gcgtttaagg
    11521 cacagcttgc acagagaatg tcagaggtta ttcaggggct gccgcctcag gtgcaggctg
    11581 ttgtgctcga cctgtgggtt aatcttctgg atgtgccgca gaaacaggag tttgttgagc
    11641 gtattcgtgc tgcgctgggg acgccaaaat caccggatga aatgacgccg gaagaacagg
    11701 aagtagcggc acaacaacag gcacttcagc aacaacaggc agaactccag atgcgcgaga
    11761 tggctggcag agtggcaaaa ctggaagctg acgccgccag ggcacatgca gctgcacaac
    11821 gggataatgc cagtgcacag cgggaagtcg ccctgacaca ggggcagcgt tatgtggatg
    11881 cgcttaacca ggcacatacg gcagaaatca ttaccggcgt acagaatatg gaacaggagc
    11941 aggacgttct tcagcaacag atgctgtata cgttacaaca gcggatgaat gaaatgtcgc
    12001 tctgaaaact ctggcttcaa ctgaaccccg tcatcgtacg gggttttttg tttccggagg
    12061 taagcgttcc gggagcggtg cgcttattcg cgggggcagc gataagcctt atttactcaa
    12121 ccattcggat ctgtccgata aacagaccat gcggagttat ttatggattt tgaatttacg
    12181 ggtgaagaaa ccccggaaca actggaaaaa atgctggaag gacttgggga tgtggatatt
    12241 gacagtcacg cacaggacgt cgtgacggaa gataccacgg aaaaacatgc ggatgaggaa
    12301 gcacagactc agacgggcga taacaatgtg gcaccgacgc cggatgccag tgtggagcag
    12361 acgcaggacg tgaaggagcc ggaagcgaag ggggtgctca cccgcgacgg taaacacgtc
    12421 attccctatg aagtccttga ggctgaacgt tccggtaagc aacgggccga acaggaagcc
    12481 gcacttcttc gtgggcagat agctgaagaa aaacgcaggg tggaactgct gacgtctcag
    12541 atccaccagg ccggtatgaa gcccacaccg ttaccggaaa acgaaaaaat ttctgatgag
    12601 cagattgccc gtatcaggga gatgtatccg gaaattggtg acgcggtggc ttcgctcatc
    12661 cgtaaaaata actatctcca gtcccgtgtt cagcaatcaa cacagcaggc agaaggtaat
    12721 ggtggtgagg atttatcacc ggttcttgat gcgatgaatg ccgtgccggt gctgaaaacg
    12781 tggcaggagt ccgatccgga tcgcttctcg gttgctgtat ccatcgacgg gaagctccag
    12841 aatgaccccg catggaaaga caaaacgctc actgaacgtt tcgctgaagt ggcccgtcgt
    12901 acgcaggttg ctttcggtga agtcagtgag tcgtctgctg acaaccaggc agacaaaacg
    12961 gatatccgga aaacggcgga agaaaaagtg aagaccgctg aacaggagca ggcagtacct
    13021 gcttccccgt cagatttagg caccacggcc tccgtcggaa ccggtgataa ttttgaacgg
    13081 ttacttggtg cttctcattc agaggctgag gcgattatgc gtggtatgac gaatgctgaa
    13141 atagacgcac ttctggagaa gctcgggtaa cttactgaag gagtactgaa gtaatgacga
    13201 ctgtaacatc agcccaggcg aataagctgt atcaggtggc gctttttact gctgccaacc
    13261 gcaaccgctc gatggttaat atccttactg aacagcagga agcgccaaaa gcggtttcgc
    13321 cggacaagaa aagcacgaag cagaccagcg caggtgcgcc ggttgtccgt atcacagacc
    13381 ttaacaaaca ggccggtgat gaagtgacct tcagcatcat gcacaaactc tcaaaacgtc
    13441 cgacgatggg agatgagcgt gttgaaggtc gtggtgagga tctcagccat gctgacttct
    13501 ccctgaaaat caatcaggga cgtcacctgg tggatgcagg cggacgtatg agtcagcagc
    13561 gcacgaagtt taacctggca tcctctgcca gaacgcttct ggggacgtac tttaatgacc
    13621 tgcaggacca gtgtgcgata gtgcatcttg ccggagcacg tggtgatttt gttgctgacg
    13681 acactattct gccgacagcg gagcaccctg aattcaaaaa aatcatgatc aacgatgtac
    13741 tgcctccgac acatgaccgt cacttttttg gcggtgatgc gacaagcttt gagcagattg
    13801 aagcggcaga tattttttct attggcctgg tggacaatct ctccctgttc attgacgaaa
    13861 tggcgcatcc gttacagccg gttcgtctgt ccggtgatga acttcacgga gaagatccat
    13921 attacgtcct gtacgtcacg ccgcgtcagt ggaatgactg gtacacctcg acgtccggta
    13981 aggactggaa ccagatgatg gttcgtgccg tgaaccgtgc aaaaggtttt aatcatccgc
    14041 tgttcaaagg tgaatgtgcg atgtggcgca atatcctggt tcgtaagtat gcgggtatgc
    14101 cgatccgttt ctatcagggg tcaaaggttc tggtatcaga gaataacctg acggcaacca
    14161 cgaaagaggt cgctgctgca accaatattg accgcgccat gttactgggg gctcaggcgc
    14221 tggcaaatgc ttacggtcag aaggcgggcg gtcacttcaa catggttgag aagaaaacgg
    14281 atatggataa ccgtactgag atagcaatca gctggatcaa cggtctgaaa aaaatccgtt
    14341 tccccgagaa gagcggcaag atgcaggatc acggcgtgat tgccgttgat acagcagtga
    14401 agctctgatt ttttcctttc cccatgccgg gttttcgccc ggctttttca ggagtcatta
    14461 attatggcaa agactatcct tgccccgtca ctgagtgaac gggtctatac gggtacgcac
    14521 ggtaatgagt cggtggcaga aggcgtattt acggtgaatg ctgcggaagc ggacagtgtt
    14581 attcatcttc tctcactgcc agtgggcatc cgtatcaact cactccagct ggtttcaacg
    14641 ggtggtctgg gtactgcaac cgtcagcatt aagtccggtg agcatgctct catcgataac
    14701 agcgaagctg tttctgcaaa atttgccaga tatgtgccag tggagccgta caccacacag
    14761 cgtgacgggg agctggttac tgtcaccatt aagactgccg ctgcaaccgg caccctgaat
    14821 gttctgctgc gttataccgt ggtgggatac tgattaaaac cttccggccc gcgtcatgcg
    14881 ggctttttat ccggggaatt atatgagtga gaaaattgcc gttgtctata tcggcccaaa
    14941 acccgtgaaa aaggacacca ttaccggaag ccgcacattg ttcccacgtc ttgagccggt
    15001 gcatgttgac agtgcgatgg cctggcaact gctggggttt ccggatgtct gggttcgtca
    15061 tgaagagctt gatgatgttc tgaaaaagca acaacagaat gagcagttgc ggcaggcaca
    15121 gcaggcgcag gaaagcgtgc ttgctgcgcg ggcagaagcg gagaacagtt ttgttgtttc
    15181 tgttaacggg caggaggtgg atttaagtaa gctcacctca gcacggctgg cgacgctgtg
    15241 tgaggcagaa gagctggata ttcacaaaga cccgaaagaa acggctgagg cattccgtat
    15301 ccgggtgcgt gaggcatttc gccgtcgtgt tgcggagact gaacagcatg gcggaactga
    15361 gtgatttttt accgtatgtc cgtcgtcata tcagcggtcc actgaacatt atgatgacgg
    15421 atgctctgtc aatggctgcc gtggcattca gccgccagtc gttggtgtgc cgtcgggagg
    15481 ttactgttgt accggtggca ggaaaagaaa tcgtgcttcc gtatgacaaa gatgatgagg
    15541 agtgcgttca tatcatccgt atctctgacg ataatcatga gctttttgtc ggtcgggatg
    15601 tggatatcag ctccggacgc tccctgcgat ttgcctgttc tcccggtgag gtgagcgtgc
    15661 tttatgccgt cgctccgaaa gccggacgca gccagatacc ggatgaactc ctcacatggc
    15721 ctgaagaagt ggctgcgggg gcacttgagc ggttgttcat gcagactggt gtttcatggt
    15781 cagatccgtt acgcgcacag tatttttctg tgcagttttc tgaggggatc cgtcgggcat
    15841 atcgtcatac actggcgaca agcccgtact cttcataccg caaccctgta cgcaggcaga
    15901 ggttttactg atgacgacga ttacagaaat catcggacgt gtgaatacac aactggttga
    15961 cccgatgatg gttcgctggc ccctgcagga attgtgcgat tattacaatg atgctgtgag
    16021 ggcagtgatt ctggcgagac cggatgctgg cgcaagcctg gaaacaatca gttgtgttcc
    16081 tggcgcccgt caggttttgc ccgatggtgt aatacaactt cttgacgtga tatgcctcag
    16141 tgatggtagt gcagtcagac cattatcccg ggaggtgctg gatgcgcagt atcctgagtg
    16201 gcccacaatg aagggcattc ctgaatgttt tatcagcaac gacctgtccc cgcgcgtatt
    16261 ctggctgttt cctgtccctg acaaagagat aagtattgat gcagtggtaa gccggatacc
    16321 ggaggcagtg tatgttctga cgcaggacga tgatacgcca gttccactgg aagaggctta
    16381 tgttaaccca ctggtggact ggatgttgtt tcgtgctttc agtaaggatg ctgccggtgg
    16441 tgcagaatcg gggctggctg cgcagcatta tcagagtttt gttgagcaac ttgggatcaa
    16501 acagggggca gacagtgcat tgtctgcccg taaaaaagtg tttaacggag gtggagtgtg
    16561 agtgttgttg tttcggggac gctgaaatct cctgatggtg aggcgatatc aggagcaaat
    16621 attaccctga cggcgctgac agtttcaccg gatgcgctca gcggcaccag tgcgtcggca
    16681 gtgacccgtg aaggtggata ttacggaatg acgatggatc cgggggagta tgcggtttcg
    16741 gtgacggtga aagggaagac tgctgtctac ggacgtgtgc gtattgaggg gaccgaaagt
    16801 acggtgacgc tcaatatgct gttacgccgc agtcttgttg aggtgagcat acccggagag
    16861 ctgctgacag atttccggca gatacagaat aatgtggctg atgaccttgc tactattcgt
    16921 cgcctgaatg aagacacggc gacaaaaaac actcaggcca cacagtcaaa agaaagtgca
    16981 gcagccagtg cgaagagtgc atctgacagt gcaaagacgg caaccagcag ggcggctgaa
    17041 gccggacaaa aagccagtga agctgcagaa gcagcgaccc gtgcaatcac agcagcgggg
    17101 aatgcagagg aaagctcgac ccgtgccgga gagtctgaaa aagccgccgg agctgatgca
    17161 gaaaaagcca gactgcatgc tgaaaaggcc aggctggcgc aggagagcgc cggagagatc
    17221 cttaagcggg cagaggctgc cactgtcagt gctgaagagg ccagacgtat ggctgagaat
    17281 gcacgggggc cccgggggcc tcagggagaa actggtccga agggggatgt cggtcctaaa
    17341 ggcgaaacag gtccagtggg ccctcaaggg cccgcagggc cgaaaggtga gcgtggtgac
    17401 gttggtgctc agggggcagt agggcccgct ggtccgcggg gtgagaaggg cgaacagggg
    17461 gagcgaggac cgcagggaat accaggcctg a
//
LOCUS       DAZGHF010000100        17455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGHF010000100 DAZGHF010000000
VERSION     DAZGHF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.100"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(96..827)
                     /gene="rlmB"
                     /locus_tag="ACHK3A_003476"
     CDS             complement(96..827)
                     /gene="rlmB"
                     /locus_tag="ACHK3A_003476"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO8741515.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(918..3359)
                     /gene="rnr"
                     /locus_tag="ACHK3A_003477"
     CDS             complement(918..3359)
                     /gene="rnr"
                     /locus_tag="ACHK3A_003477"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO8741516.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3398..3823)
                     /gene="nsrR"
                     /locus_tag="ACHK3A_003478"
     CDS             complement(3398..3823)
                     /gene="nsrR"
                     /locus_tag="ACHK3A_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO8741517.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4028..5326)
                     /gene="purA"
                     /locus_tag="ACHK3A_003479"
     CDS             complement(4028..5326)
                     /gene="purA"
                     /locus_tag="ACHK3A_003479"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO8741518.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5430..5627)
                     /gene="yjeT"
                     /locus_tag="ACHK3A_003480"
     CDS             complement(5430..5627)
                     /gene="yjeT"
                     /locus_tag="ACHK3A_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO8741519.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5709..6713)
                     /gene="hflC"
                     /locus_tag="ACHK3A_003481"
     CDS             complement(5709..6713)
                     /gene="hflC"
                     /locus_tag="ACHK3A_003481"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO8741520.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6716..7975)
                     /gene="hflK"
                     /locus_tag="ACHK3A_003482"
     CDS             complement(6716..7975)
                     /gene="hflK"
                     /locus_tag="ACHK3A_003482"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO8741521.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8061..9341)
                     /gene="hflX"
                     /locus_tag="ACHK3A_003483"
     CDS             complement(8061..9341)
                     /gene="hflX"
                     /locus_tag="ACHK3A_003483"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO8741522.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGEGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9418..9726)
                     /gene="hfq"
                     /locus_tag="ACHK3A_003484"
     CDS             complement(9418..9726)
                     /gene="hfq"
                     /locus_tag="ACHK3A_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO8741523.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9812..10762)
                     /gene="miaA"
                     /locus_tag="ACHK3A_003485"
     CDS             complement(9812..10762)
                     /gene="miaA"
                     /locus_tag="ACHK3A_003485"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO8741524.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10755..12602)
                     /gene="mutL"
                     /locus_tag="ACHK3A_003486"
     CDS             complement(10755..12602)
                     /gene="mutL"
                     /locus_tag="ACHK3A_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO8741525.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTVEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKMVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPLYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLMPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12612..13949)
                     /gene="amiB"
                     /locus_tag="ACHK3A_003487"
     CDS             complement(12612..13949)
                     /gene="amiB"
                     /locus_tag="ACHK3A_003487"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO8741526.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPSPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTFLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13968..14429)
                     /gene="tsaE"
                     /locus_tag="ACHK3A_003488"
     CDS             complement(13968..14429)
                     /gene="tsaE"
                     /locus_tag="ACHK3A_003488"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO8741527.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14401..15948)
                     /gene="nnr"
                     /locus_tag="ACHK3A_003489"
     CDS             complement(14401..15948)
                     /gene="nnr"
                     /locus_tag="ACHK3A_003489"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO8741528.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15947..17086
                     /gene="queG"
                     /locus_tag="ACHK3A_003490"
     CDS             15947..17086
                     /gene="queG"
                     /locus_tag="ACHK3A_003490"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO8741529.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17069..17122)
                     /locus_tag="ACHK3A_003491"
     CDS             complement(17069..17122)
                     /locus_tag="ACHK3A_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741530.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17356..17431)
                     /locus_tag="ACHK3A_003492"
     tRNA            complement(17356..17431)
                     /locus_tag="ACHK3A_003492"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17396..17398),aa:Gly,seq:gcc)
BASE COUNT         3961 a         4979 c         4379 g         4136 t
ORIGIN      
        1 taataaaatg aatatttgca gtacgtaatt atcttaccag ctatagacaa aaaaaaacca
       61 tccaaatctg gatggctttt cataattcag aggaactagc tgcgctgacg aaccgcttca
      121 aataagcaaa ttccggttgc aaccgaaacg ttcagggaag aaacacttcc tgccattggg
      181 atgctgatca actcatcgca atgttcacgg gtcaggcgac gcataccttc accttccgcg
      241 cccatcacca gcgccaggcg tccggtcatt ttgctctgat agagtgtatg atccgcctcg
      301 cctgccgtac cgacgatcca gatattctct tcctgcaaca tacgcatggt gcgcgccaga
      361 ttagtcaccc gaatcagcgg aacgctttct gccgcaccac aggccacttt tttcgccgtg
      421 gcgttgagct gtgcggagcg atctttcggc acaatcaccg catgaacacc agcggcgtcc
      481 gcgctacgca ggcacgcgcc gaggttgtgc ggatcggtta cgccgtcgag gatcagcagg
      541 aacggttgat cgagcgaagc gatcagatcc ggcagatcgt tttcctgata ctgccgtcct
      601 ggcttcacac gagcgataat gccctgatgc acggcaccgt cgcttttctc gtcgagatat
      661 tggcggtttg ccaactggat aaccacgccc tgagactcaa gggcgtgaat cagcggtaac
      721 agacgtttat cttcacggcc ttttaaaata aagacttcct gaaaacgttc aggggcgcgc
      781 tccagcaggg cctgcactgc atggatgccg taaatcattt cgctcattaa tgtactcgtt
      841 atttacgttt tgccgggagc gccacgcttg cccggcctac ggataattca ccctcttctg
      901 tttgaagagg gtaatgatca ctctgccact tttttcttcg ccgcacgctt cgctttggtc
      961 gctgcggcta ttttctgcgt tttcgccgat ggctttttcg cttttctcgc gtctttcttc
     1021 gctgctttcg gcttcggttt tttctcgccg cggaaggcgc tgtctggctc aaagttgacc
     1081 tttttgccga cctgacgccg cttgccgcct tttttgcctg catcgccttt tttcgctttc
     1141 tcgcgcgccg ttttaccgac gttgcgcggt gcgcgttcgc tggagatcag gctaaagtcg
     1201 attttgcgct cgtccatatt aaccgcttcg acgcgaactt ccacgcgatc gcccaggcga
     1261 taagtctggc cgctggattc gcccatcagg cgttgcccca cctggtcaaa gcgatagtag
     1321 tcattgtcca gcgaagagac atggaccaga ccatcaatga acaagtcatc caggcgaaca
     1381 aagaagccaa aaccagtgac gctggaaatt acgcctttaa agacgttacc tacctggtcg
     1441 agcatgaagt cacacttcag ccagtcagcc acatcgcgcg ttgcttcgtc ggcacgacgt
     1501 tccgccatcg aacagtgctg acccagttgc agcatctctt ccatcgaata atggtagccg
     1561 ccggtttcag tggtattccc ctgatgcccc tgctctttcg ccaacagata cttaatggcg
     1621 cggtgcagcg tcaggtcagg ataacgacga atcggcgaag taaagtgcgc ataggactgc
     1681 aatgccagac cgaagtgacc acggttttcc ggatcgtaaa tcgcttgttt catcgagcgc
     1741 agcagcatgg tttgcagcat ttctgcatca ggacggtcgg caaccgactc cagcagctct
     1801 gcgtagtcgc gcggttccgg cttgttaccg cccggcagtt ccagccccag ctccgccagc
     1861 actgaacgga aagaggtaat cgcttcggtg ctcggcttgt cgtgaatacg gaacagtgcc
     1921 ggttctttcg ctttctcaac gaaacgcgcc gccgagatat tcgccagaat catgcactct
     1981 tcaattaact tgtgtgcgtc gttacgctga gtctgttcga tacgctcaat acggcgttcg
     2041 gcgttgaaaa tgaacttcgc ttcttcgctc tcaaatgaga tcccgccacg ttcttcacgg
     2101 gctttatcca gcactttata gaggttatgc aactcttcga gatgcttaac cagcggggca
     2161 tactgctcgc gcagatcctg atcgccttgc agtatatgcc agactttggt gtaggtcaga
     2221 cgcgcgtgag aactcatcac cgcttcgtag aatttgtagc cagtcaggcg gcctttcgac
     2281 gaaaccgtca tctcgcacac catacacagg cggtctacct gcgggttgag cgagcacagg
     2341 ccgttagaga gcacttccgg cagcatcggg ataacctgcg aagggaagta caccgacgta
     2401 ccacggttac gcgcttctct gtccagcggt gttggcggac gcacatagta actgacgtcg
     2461 gcaattgcga cccataaacg ccagccgccg ccgcgttttt tctcacagta aactgcatcg
     2521 tcaaagtcac gggcgtcttc gccatcaatg gtgaccagcg gtaaatcgcg cagatcgaca
     2581 cggcccgctt ttgcttcttc cggcacttct tctttcagcc cggcaacctg ttgctcaaca
     2641 gcctgcggcc agatgtacgg aatttcatgg gtacgcagag caatatcaac cgccatgccg
     2701 gtgcccatat tatcgcccag cacttcgacg attttaccca ccgctttggt acggcgagtc
     2761 gggcgttgag tcagttcaac caccaccaca aagcccatcc gcgcgcccat gatctgatcg
     2821 ggcgggatta agatatcgaa gctcagacgg ctatcgtcag gaaccacaaa gccgacgccc
     2881 gcttcggtga agtagcgacc aacaatctgg ctggtttttg gcaccagtac gcggacaata
     2941 cgcgcttcac gacgaccttt acggtcagcg cccagcggct gagccagcac ctgatcgcca
     3001 tgaatgcagg ttttcatctg ctcgctggag agatacaaat catctttacg cccttcaact
     3061 cgcagaaagc cgtagccatc acggtggcca ataacggtac ctttcaccag gtcgaggcgt
     3121 tccggcagcg catagcactg acggcgagtg aagaccagtt gaccatcgcg ctccatcgcg
     3181 cgcaggcggc gacgcaggcc ttcaagctgc tcttcgcctt caatgtgcag ttctaccgcc
     3241 agctcatcac ggctggccgg tttttcacgt ttggttaaat gttcgaggat aaattcccga
     3301 ctagggatgg gattcgcgta tttttcagct tcgcgttcct ggaaaggatc ttgtgacatc
     3361 tcggttcctc cgttgtcatc tctgatgaag attttcgtca ctccaccagc aataatttat
     3421 aaagcggttg attctcttca accaaatcgg caagcgtgta gttatccagt tccgtaagaa
     3481 aactttgcac ggccttagaa agcgcctgtt tcaaccgaca ggcaggtgta atgtggcaaa
     3541 actcactgct gcaattcacc agcgataagg gctccagctc gcgcaccaca tcaccaatac
     3601 gtattgcact cgccggttta cccaagcgaa tgccgccatt ttttccacga acagcagtca
     3661 cgtagccggc acgactaagt tgattgatta ttttgaccat atgattacgg gagacgccgt
     3721 agacgtcagt cacttcagaa atactggtca tccgcccttc tggcaatgac gccatgtaga
     3781 tcagcgcacg taatccgtaa tcagtgaaac tcgttaactg cacatcaacc tcaaaaggga
     3841 aatcggggaa aaatacattt atattgatga taaaccagcc acaagctgtg tcgctaattt
     3901 atttcagttc ggggaaggaa aaaagcgagg atttaacact gtgtcggata gcgtaatggc
     3961 ttaaccgaca cagcaatgta tcacactgtt agcccggcag gcaaaatatc tgccaggcgt
     4021 accagaatta cgcgtcgaac gggtcgcgca gaatcatggt ttcagtacga tccggaccag
     4081 tagagatgat atcgatcggc acaccggtca gctcttcaat gcgcttgatg tagttcagtg
     4141 ccgcctgcgg caggccgcta cgatctttca cgccgaaggt ggattcagac cagcccggca
     4201 tggtttcgta aatcggctct acacctttcc agtcgtcagc tgccagcgga gtggtagtca
     4261 cttcacgacc atccggcata cggtaagcca cgcagagttt aacctctttc aggccatcca
     4321 gaacgtccag tttagtcagg cagaagccag acagagagtt cagctgtacc gcacgacgaa
     4381 cggcaacggt gtccagccag ccggtacgac gacgacgacc cgtagttgcg ccgaattcgt
     4441 taccctgctt gcagaggaac tcgccagttt catcaaacag ttcggtcggg aacggacctg
     4501 cacccacacg agtggagtaa gctttgagga tacccagaac gtaatcaaca taacgcgggc
     4561 ccaggccgga accggtcgcc acgccaccag cagtggtgtt ggaagaagtt acgtacggat
     4621 atgtaccgtg gtcgatatcc agcagcgtac cctgcgcacc ttcaaacatg acgaaatcgc
     4681 cacgctgacg cgcctggtca agcaggtcag aaacgtcaac aaccatagaa gtcaggatgt
     4741 cggcaacagc catcgtatca tccagaactt tctggtaatc aaccgcttca gctttgtagt
     4801 agttaaccaa ctggaagttg tgatattcca tcacttcttt cagtttttca gcgaaggttt
     4861 ctttgtcgaa aaggtcgcca acacgcagac cgcgacgtgc cactttatct tcataagcag
     4921 gcccgatacc tcgaccggtg gtgccgatcg ctttcgcgcc acgcgctttc tcacgcgcgt
     4981 tatccagcgc aacgtgataa tcaaggatca gcggacatgc ttcggacagc agcagacgct
     5041 cacgaacggg gatgccacgg tcttccagtt ctttcatctc tttcatcagc gcagccggag
     5101 acagcacaac accgttaccg atgatgctgg ttacattctc gcggagaata cctgatggaa
     5161 taagatggag aacggttttt tcaccgttga ttacgagagt atggcctgcg ttgtgaccgc
     5221 cctggtagcg tacaacatat ttagcccgtt cagtcagaag atcgacgatc ttacctttac
     5281 cttcgtcacc ccattgggtg cccagtacga cgacgttgtt acccattttt caaaatcacc
     5341 gtttgcttaa aaatggattc taccatcgct ttttcagagt tacagcactt tttgcactca
     5401 aaaatgacct cgatcagtct gttttttgtt cagccaatcg ttttcctcaa catgtagtag
     5461 accacaacgc ccgcaaccac aagtccaccg ccaaaacgac gtaaaatatt atcgggcaaa
     5521 ttggtcatcg cagagatcat cttcttccat gccttcgggt aaagcatcgg ccctaaacct
     5581 tccagtacca aaaccagggc aagcgccagc cagattgtcg aattcattca ttatccttat
     5641 agaaaaagaa aaccaccgac atccctgagg atgcggtggc tttattgacc tgtaccgcag
     5701 tcgttatatt aacgcgttgc ggaagtcggc gtcttcatgt agcggaagaa atcgctatcc
     5761 gggctcatga ccatcacgtc ctgattgcca gagaagctgt tctcataagc acgcaggcta
     5821 cggataaatg cgtagaagtc cggatcttta ctgaatgcat cagcaaacag tttggctgct
     5881 tctgcatcgc cttcaccacg catgatgcgg ccctgacgct cagcttcagc cagcgttctg
     5941 gtcacttcat agtcggcagt cgcgcgcagt ttttccgctt cttcctgacc ttgtgaacgg
     6001 tgacgacgcg ctaccgcttc acgctcggcg cgcatacggt tgtagatcgc ttcagacact
     6061 tcggtcggca ggttgatctg cttaatacgc acatcgacaa cttcaatacc cagcgccgcc
     6121 atactgttcg ggttgatgac cggaactttg cccttcgtct cagccgttac gcgctctgcc
     6181 gcttcggcaa tggcgttatc tgctgccggg gtagtaactt catcttctgt acccgcagaa
     6241 ccggagttca gcgcgtcacg tacttcgagg gtcagacgac cacgggaatc ggtgacgatg
     6301 tctttcacgt ccaggcgacc aatttcagaa cgcagacggt cagagaactt acgtttcaac
     6361 agcacttccg cttgcgaaat gtcgccacca cccgttgcca ggtagtaacg gctgaaatcg
     6421 ctgatgcgcc atttgatgta agagtcgacg atcaggtctt tcttctcttt ggtcacaaag
     6481 cggtcggcct ggttgtccat ggtctgaata cgtgcgtcga gcattttcac cgtttcaatg
     6541 aacggtatct tgaaatgcag acccggctca taaaccagag gtttgttgtc atcgtcacgc
     6601 agtaccttac caaaacgcag cgtaataccg cgctcacctt ctttgacgac aaagacagac
     6661 atgtaaagca ctaccagcac gatgatgata atcgcgataa ctgacttacg catcgttatt
     6721 ccccctgacg ctggtagtcg ttacgctgcg cgttggcgcg gcgttggtcc ataatatcgc
     6781 cctgactggt ggacgacgtg ttgcttgctc cactggttgt ggaggaagag gctggcggca
     6841 gacgcagcag attgctggca ccgttatcgc tcttcgccgc aggggcgtta ccacctttca
     6901 gcatctggtc taacggcaga accatcaagt tgccaccttt atcgttaacc agcactttgc
     6961 gggtgttgcc caacactttt tccatcgtct cgatatacag acgctcgcga gtaatttccg
     7021 gcgcggcttt atattccggc agaagtttag caaagcgcgc cacttcaccc tgagcttcca
     7081 ggatggtctg ggccttgtac gcacgcgcct cttcgaggat acgttgcgcc tgaccgttcg
     7141 cacgcggctg aacttcgttg gtatacgctt ctgcttcacg aatgtattgc tgttcgtttt
     7201 cacgcgcggc aatcgcatcg tcaaacgccg cttttacttc ttccggcgga cgagctgcct
     7261 ggaagttgac gtccagcagc gtgataccca tgtcatacgg acgaatcgtc tcttccagtt
     7321 cgcgctgagt atcgctacga atcacggtac gaccttccgt cagaatgcgg tccatggtgt
     7381 atttaccgat aaccccacgc agggcgctgt cggtagcctg acgcaggctg tcatccgggc
     7441 tggtcacgct atacagatat ttttccggat cggtgacgcg gtactgcacg ttcatctcaa
     7501 cgcgcactac gttctcgtcc gacgtcagca tcacaccaga agcggccagt tcacgcacgg
     7561 cttccacgtt caccggtttg acttcgtcga taaacgtcgg tttccagttc agacccggct
     7621 caaccagatg gctgaattta ccaaagcgtg ttaccacgcc acgttcggct tctttaatgg
     7681 tatagaaacc actggccgcc cagataatga caatcgctgc cgctgcgatg gtaacgacac
     7741 gaccgccaag ctgcgggcgc gggccttgcg atgaactgcc accgccagat ccggtgcctt
     7801 taccgccgcc cagaccaccg agctttttgc tcagtttgcg gaagatatca tctaaatcag
     7861 gtggcccttg atcgcgaccg cctttgtttc catttccctc agagttgccg ccaggtttgc
     7921 tgcttcccca cgggtcgcgg tcttgtccgt tattaccggg ctgattccac gccatgtttg
     7981 tgctccatat ttgttatgcg gtgatccctg ttgtctttcg acgccagagg atatgactcc
     8041 acgcttcaga cgctacgccg ttagatcagg taatcgatca acgccggttc ttgtttacag
     8101 aggcgacgcc agtcaacgat cggcatacga acttgcagac ttacgctgcc gtcctcctcc
     8161 atccactctt tttctattgc ctgaagctga taaaaacgac ttctcagacg cccttcctgc
     8221 ggtggcagac gcaatgtatg ctgcgccacc tcgccggaaa gccgctccgt caaagcctga
     8281 aaaagctgtg gtatcccctc tccggtctgt gcggaaagcc agacacggat cggtttgttc
     8341 tcttcgtccc gatcaatacg cggttcgaaa tcttccagca tatcgatctt gttcatcacc
     8401 agcagggttg ggatctcgtg agcgtcgatc tcttcaagaa ccgtattcac cgcttcgatg
     8461 ttttcttgta cacgcacatc cgccgcatca atgacgtgca gcagcaatgt ggcttgccgc
     8521 gtctcttgta acgtggcttt aaatgccgcc accagatcgt gcggcaggtg gcgaataaac
     8581 cctacggtat ctgccaacac ggtttcaccg acatctgcga cgtcaatacg ccgcaacgtc
     8641 gggtcgaggg tggcaaacaa ctggtctgcc gcgtagaccc gcgcttcggt gatgcgattg
     8701 aaaagggtag atttaccggc gttggtatat cccaccagcg aaacagtagg aacgtcggct
     8761 ttgatacgcg attgccgccc ctgctcacgc tgcttttcaa ctctttccag gcgcgactgt
     8821 atctgcacga tgcgattacg caacaaacga cggtcggttt cgagctgggt ttcacccgga
     8881 ccacgcaaac ctatcccgcc tttctgtctt tcaaggtggg tccagccacg caccaggcgc
     8941 gtagccagat ggcgcagctg cgccagctca acctgcaact taccctcatg ggtacgcgca
     9001 cgttgggcga aaatatctaa aataaggccg gtgcggtcga taacacgaca ctcgcacaaa
     9061 cgctccaggt tacgctcttg cgccgggctc agggcatggt caaaaagaac gaccgaagca
     9121 cccgtcgctt tgacagcttc cgcaatttca actgctttac cttcacctac aaaatacttt
     9181 gggtgcggcg ctttacggct accggtaatc acctgcaatg cttcgacacc ggcggaagag
     9241 accagagatt caaactcctg gaggtcttcc atatctttgt cttgcgtaaa atagatgtgt
     9301 accagtaccg cctgctcacc agcatcataa cggtcaaaca agcgtataac cctctaaata
     9361 gatcagcggg gaacgcagga tcgctggctc cccgtgtaaa aaaaacagcc cgaaacctta
     9421 ttcggtttct tcgctgtcct gttgcgcgga agtattctgc gcgctgctac catgatggta
     9481 gttactgctg gtaccgccac cggcgttgtt actgtgatga gaaaccgggc gagacgggac
     9541 aacagtagaa atcgcgtgct tgtaaaccat ctggctgacc gtgtttttca acaggatcac
     9601 gaactgatca aaagactcga tttgcccttg cagcttaata ccattcacca aataaataga
     9661 aactggaaca cgttcccgac gcagtgcgtt caggaacgga tcttgtaaag attgcccctt
     9721 agccattctc tcttttcctt atatgcttat ttgtactttg aacctttcga ttctgaaaaa
     9781 attgcgcacg atacgtctca attgtacaca ttcagcctgc gatagcacca acaacctgta
     9841 atacttcgtc acgcgcttgt tctggttttt cactgtcaag ccagtgaacc ccttcccaac
     9901 cacgcagcca ggttatctgc cgcttcgcca actgtctcgt ggcgcaaaca cctcgataaa
     9961 ccatttcatc gtatgagatt tcgccttcaa ggtaagacca catctggcga taacccacgc
    10021 aacgaatgga aggcaaatcc gtatgcaaat ctcctcgggc aaaaagcgcc cggacttctg
    10081 cttcaaaacc tgaagccaac atctgatgaa aacgctgctc aattcgttga tggagcagtt
    10141 cacggctcgc cggggcgatg gcgaactgat gcacctgata cggtagagcg tctcctgacg
    10201 tttgcgtcag ttccgttaaa gttttacccg aaatgaaaaa aacttccagt gcccgggaaa
    10261 gcctttgtgg atcatttgga tgaatccttg ctgccgcaac cggatctacc tcctgaagtt
    10321 gacgatgcaa tgactcccaa ccttgctctg ccgcctgttg ctcaattctg gcccgcactt
    10381 ccgggtctgc cgacggtagc ggcgacaacc cttccagcaa tgccttgaaa tacaacatcg
    10441 taccgcccac taacagtggg atccgccccg ccgcggtgat atcggccatt tccgccagcg
    10501 catcgcggcg aaaatcagca gccgagtaag cctgcgacgg atcgcgaata tccagcaatc
    10561 ggtgcggcgc ggcgagtaac tcttcagcgt tcggcttcgc cgtcccgata tccatccctt
    10621 tgtaaataag ggcagaatca acgcttatca actctactgg taaaatttta cgcagctcaa
    10681 tggctaacgc cgttttaccg gaggccgtcg gccccatcaa aaaaatcgcc ttaggcaggc
    10741 tcgccttact gatatcactc atctttcagg gcttttatcg ccggatgtaa atcaacagat
    10801 tgtaacagac cacccggcgg cgttttcaca agttgcggac ataaccgttc cacgtccgcc
    10861 agcagggtta tggcctgcgc cattgaccac tgcgcatgtt cgctcatcag atttcgtgca
    10921 atccactgcg caatattgcc aggttcgaat acggactgct tcgccaggta gcctatcagt
    10981 tcaggaatca agatttgtaa attttgttgg cgtaagggta aaggcacggc cctgatggtc
    11041 acatgctgcg catcggactg gaaatcaata cccaattccg ccagggcaga ctgcgctttt
    11101 tctaatgcag atttttcttc gccagaaact tttagccgca gcggcatcag caacggctgg
    11161 gcgcaaacgg gcgcttcacc cggcgtcaat tgtgcctgac gcagccaacg ttctgccact
    11221 ggcaaggcta aaagtgaaat gttgccgtcg cgctccagca acgcacagtc ggaatggacg
    11281 atagtcagta cccgaccaaa gctttgactg ttcgccgcaa gtgcaggttc ctgcggttct
    11341 ggcgcttttg gtttttgcat cggcgcgggc gtttgcaaaa gctggcgata cacttcacct
    11401 tgctgtttct ggtaacctgg ctgcgcattc ggccaggggg cagccggacg actgcctgag
    11461 gcgggcgcag gagtgtaaag cggagctact ggctcacgaa ctgccggttc agcaaaatga
    11521 ttgcgccccg ccgccacgcg gttttccggg atggcgcgcg gtgcaggttg tggttcatcg
    11581 tccagcggta gcggcgtttc cagttgctgt tgtagcacgc tcagcacgcc ctgatagata
    11641 aagtcatgca ccagacgcga ctgatggaaa cgcacttcgt gtttggcggg gtgtacgttg
    11701 acgtccacct gatgcgggtc gatctccaga tacagcacaa acgccggttg ttgatcggcc
    11761 cccagtttgt cttcgcaggc ctggcggatc gcatgattga tcaggcgatc gcgcatcatg
    11821 cgaccattca cgtagcaata ctgaatttcc gccagtgcgg gcgtggtgtg attcggatct
    11881 gccacccagc cgcgcagcgt aagatcgccg tgttgccatt caatcgccag cgcttgttcg
    11941 agaaaagcgg tgccgcaaat cgcccctaaa cggcgctctt tttgtccacc ttccggcacg
    12001 gcgcggtact ggcggaccat tttcccgtta tgcgacaggt tgatcgtgac gtcgaaacgc
    12061 gccagcgcaa tgcggcggat gatctcatca atgtggttaa attcggtttt ctcggtgcgc
    12121 aggaatttgc gccgcgccgg ggtgttgtag aacagatcca gcacctccag cgtagtcccc
    12181 acaggatgcg ccgccggttt taccgtcacg tccatatcgc gcccttcggc ataggcctgc
    12241 caggcttcct gctgttctac ggtgcgtgaa gtgagcgtca ggcgggaaac cgaactgata
    12301 ctcgccagcg cctcaccgcg aaagcccagg ctgataatgg cttcgagatc gtccagagag
    12361 gcgattttac tggtggcatg acgagccagc gccagcgcca gctcatcttt tttgataccg
    12421 cagccgttat cacgaatgcg gataagtttc gccccgccac gttcgatatc aatatcaatg
    12481 cgcgtcgcac ctgcatcgag gctgttttcc accagttctt tgactaccga cgcaggtcgc
    12541 tcgaccacct cacctgcggc aatctggttc gccagttgtg gcggtaagac ctgtattggc
    12601 atcaatcgtc cttagtttgg cagcgtacga tctggcgtcg tcaccgtact ggcagtttgt
    12661 gccgttgcac cctgcggcgc agattgcatc ggatgcgcaa ggaaataatt gcgcagacct
    12721 ttataaatgg cttctgccag ctgttgttgg taatcgtcgc tcgccagcaa acgttcttcg
    12781 ctgttgttgc tgataaaacc ggtttcgacc agtactgatg ggatatccgg cgaacgcaga
    12841 acgccaaggc tggcgtgttc tggtcgacgt ttatgtattt cgccaatgcg ttgcaactgg
    12901 ctgatcatac tggtcgctac atcataccct acacgctggg aatgaccgaa ctgtaaatcc
    12961 agcaccgcct ggcttaaata gggatcagac tgactgttcg ccagcacatc gcccgctccg
    13021 cccagcagct ccgactgttt ctcgtgctgc tccagccagc tggccatttc gctgttggcg
    13081 cgacggttag agagcaccca tacggaagcg ccagtcgcac tgcggtttgg tgcggcatca
    13141 gcgtgaatcg acacgaggaa attggcgttt tgcttacgtg ccacatcgct gcgccccatc
    13201 accgagataa agtaatcccc gtcacgggtt aaaacgcctt taaacattgg atcgtcattg
    13261 agaaaagtac gcaatttacg tgcgatggcg atggtgacat ttttctcccg cgtaccaccg
    13321 gggccgatag cgccagggtc ctgaccgccg tgtcctgcat caatagcgat gataatttta
    13381 tcgccagtgt tagccgtcgc gcgcgctgcc ggacgcgtta ccgtattact gctgataaca
    13441 cctgtagtgc ggttactttc cgttttaaac ggattgcgcg ccggttcgct gacgcgcggt
    13501 gcgacaaccg caggcgtttc aacgcgttta gcaaccacag gcggcgaagg aggcggtggc
    13561 ggcacatcgg cgttaatcgt aaagacgaca gtgtaattgc tgccattctg ccgcttcacc
    13621 gcttcggttt taccgttttc ggttagatcg accaccagcc gtagcgtttg tgcatcttta
    13681 ggcgttccgg agcgaatcgc cttcaccaga ttattgccgc taaacaacaa cggcagtccc
    13741 tgaatcacgc ccgtttgttt gatatcgagc gccacggtgc gtttgctttg atggctaaac
    13801 gcataatcag gatcgccaat aaaactcaac gttatccgcg cctgctgatt gccgttagaa
    13861 acctgaatat cagagagcgt cgcggcaccc accggcgcgc acagcagcag caacgtcgct
    13921 accaaccaat ttctgatgcg atacatcatc ccgccacctt tcaaaggtta accggctaaa
    13981 cgcgccagca acaattcacc cgcagaggaa accgcactca cgcgcgcctc acggccttgt
    14041 gcctgataat caatgtgtat ttcgacatcc gggtcaggaa gaacacctgt accttgttgt
    14101 ggccactcca ccaggcagat ggcatcgttg gcaaaataat cgcggatccc cataaactcc
    14161 agctcctcgg gatcggcaag gcggtacaaa tcaaagtgat aaaccattaa attgtcgagc
    14221 gtatagggtt cgaccagcgt ataagtgggg cttttgacat taccctgatg gcccaaagcc
    14281 tgcaaaaagc cccggctaaa ggtggtttta cctgcgccta aatcgccata cagatagatt
    14341 acggttgcgc catcgcaggc tttcgctacc cgctcgccca ggtctaatgt tgcctgctca
    14401 tcagggagcg gaattactcg attcatcatg gtttttatca gtcacttccg ggttaacaat
    14461 acgctgtagc gtggaaaaga gatcggttgc cagcatcccg cgcgttccaa aacgcgccgc
    14521 cagtacgtca gctgccgcac cgtgcgcgac acagcccgca caggctgcat catacggcga
    14581 cagtttttgc ccaagcaatg cgccaataat accagagagc acatcgccca tgccgccgct
    14641 cgccatgcct gcattcccgg catcaataat gcctaaagcg tcaggatggg cggcgaccac
    14701 ggttccggca cctttcagca ccgctacgcc gccataacgt tgtaccagac gtttggcgca
    14761 atgtaagcgg tcactttcaa tttcagcgac ggaacagcct aacaaccgtg cggcctcgcc
    14821 aggatgtggc gtgatcacgc gattgtgacg cttatcggga ttgattgcca gcaggttcaa
    14881 tgcatcggca tcccacaaca tcggtttgcg aaaattctca actttttgca gggctttttt
    14941 cccccactct tgctggccca gaccgggacc aatcaccacc acatcggccc attccaggct
    15001 ttcggtaaga gagtccatag tcagttcatg caccatcaat tctggtcgtg cagtcagcag
    15061 cggcgcaatg ttctcactgc gggtcagtac tcggactaaa ccagcaccag cacgtagcgc
    15121 cgcttccccc gtcatacgaa tagcccccgc cgtgccgtga tcgccaccga taatcaccag
    15181 ccgcccgtga tcgcctttat gcgaagtcgg gcgacgcggt atcagccact gagaaagttg
    15241 ttctgccgaa aaccgctgaa ttttcgtctc ctgacctgcc agccagctat ccagccccag
    15301 tgagtcaaaa tgcagttgtc cggtaacatc ccgcgctttt ccagtgagca agcctggttt
    15361 cagcgcaata aaagtgatgg tgtgatcggc gttgattacc gcgcctggcg tagcgccggt
    15421 ttcagccaga aggccggaag ggatatcaac cgccacaatc ggcgcagggt gagtattagc
    15481 gtggtcgatt aactggctaa tggattcgcg gggcgcttgc tgcaagccgg tgccgagcag
    15541 cgcatcaaca atcagatcta ccgattcggg ccagacaata ttcgaagcat ggatctcgcc
    15601 tcccgcgttt aaccatgctt cgcgtgccag cgcggcctct tccggcaacg gtttgtcact
    15661 ttcctgggcc agcagcgtga catcaatacc gatcgctttg gccagccgcg cgaccacgta
    15721 gccatcgccg ccgttattac catgaccgca cagcaccagc cagtggcggg cgtcaggata
    15781 agccgaacga cacacctgga atgcggcttc gccagcgcga agcatcagct catagagtgt
    15841 gagccccagc gcatctgccg cctcgcgttc tccgcggcgg atatcgtcgg cgtaccagac
    15901 ggtgtgtggt atacttacgg ggtttttctt cattgtatgg tccgtcatgt cagagcccct
    15961 cgatctcaat caattagcgc aaaatattaa acagtggggg ctggaactgg gctttcagca
    16021 ggtaggtatt accgataccg atctcagcgc gtccgagccc aaactgcaag catggctgga
    16081 caaacaatac cacggcgaaa tggactggat ggcgcgtcac ggtatgctgc gtgctcgtcc
    16141 tcacgaatta ttgcccggta cgctgcgcgt gatcagcgtt cggatgaatt accttcctgc
    16201 taacgccgca tttgccagca cgctgaaaaa tcccaaactc ggctatgtta gccgttatgc
    16261 gctgggccgt gactatcaca agcttctgcg caaccgactc aaaaagctgg gcgagatgat
    16321 tcagcaacat tgtgtttcgc tgaattttag accgtttgtc gattctgcgc ctattctcga
    16381 gcgcccgtta gctgaaaaag caggtcttgg ctggacaggt aagcactcac ttatcctcaa
    16441 tcgcgaggcc ggttcgttct tctttttagg cgaattgctg gtcgatattc cgctgcccgt
    16501 ggatcaacca gtcgaggaag gatgcggtaa atgcgtggcc tgtatgacga tttgcccgac
    16561 cggtgccatc gtcgagccat ataccgtcga tgctcgccgc tgtatctctt atctcaccat
    16621 cgaactggaa ggggcgatcc cggaagagtt gcgaccgtta atgggaaacc gtatttacgg
    16681 ttgcgatgac tgccagctta tctgcccgtg gaatcgctat tcgcaactca ctacagaaga
    16741 cgatttcagc ccgcgtaagc cgctacacgc accggaactc attgagttat tcgcctggag
    16801 tgaagagaag tttttaaaag tcacggaagg ttcggcgatt cgccgtatag gtcacctgcg
    16861 ttggctgcgt aatatcgccg tagcattagg caatgccccc tgggatgaaa cgattttgac
    16921 agcgctggaa agtcgtaaag gtgagcaccc acttcttgat gagcacatag cgtgggcgat
    16981 tgcgcagcaa atcgagaggc gaaatgcgtg cgtggtcgaa gtgcaattac ccaaaaaaca
    17041 gcgtctggtt cgggtgattg aaaaagggtt accgcgtgac gcctgattca tccacagctt
    17101 gtgaataaaa ataaaaacgt attgcaattc aagacgaata aaatcgtcaa gtgatcgact
    17161 cgacaaaacg aaatcttaat ttttaactta tctatcaata ggttaaaaaa atactattca
    17221 ccgtgcgaag tttctttgaa attaagatta cggcctgagg ctgtggataa ctctgtttac
    17281 aagagtttgt cagagacaac aaaaaccagc aataacgcat cgcgtcgctg tggatatttt
    17341 attgagagaa gaatttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17401 ggtcgtgctc taccaactga gctattcccg cttgggtggt ctgtgcttta cagca
//
LOCUS       DAZGHF010000101        17270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGHF010000101 DAZGHF010000000
VERSION     DAZGHF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.101"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(71..316)
                     /gene="zapB"
                     /locus_tag="ACHK3A_003493"
     CDS             complement(71..316)
                     /gene="zapB"
                     /locus_tag="ACHK3A_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO8741531.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            742..1587
                     /gene="glpF"
                     /locus_tag="ACHK3A_003494"
     CDS             742..1587
                     /gene="glpF"
                     /locus_tag="ACHK3A_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO8741532.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            1610..3118
                     /gene="glpK"
                     /locus_tag="ACHK3A_003495"
     CDS             1610..3118
                     /gene="glpK"
                     /locus_tag="ACHK3A_003495"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO8741533.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            3289..4299
                     /gene="glpX"
                     /locus_tag="ACHK3A_003496"
     CDS             3289..4299
                     /gene="glpX"
                     /locus_tag="ACHK3A_003496"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO8741534.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            4396..5142
                     /gene="fpr"
                     /locus_tag="ACHK3A_003497"
     CDS             4396..5142
                     /gene="fpr"
                     /locus_tag="ACHK3A_003497"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO8741535.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(5147..5575)
                     /gene="uspD"
                     /locus_tag="ACHK3A_003498"
     CDS             complement(5147..5575)
                     /gene="uspD"
                     /locus_tag="ACHK3A_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO8741536.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(5602..5901)
                     /gene="yiiS"
                     /locus_tag="ACHK3A_003499"
     CDS             complement(5602..5901)
                     /gene="yiiS"
                     /locus_tag="ACHK3A_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO8741537.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(6113..6553)
                     /gene="yiiR"
                     /locus_tag="ACHK3A_003500"
     CDS             complement(6113..6553)
                     /gene="yiiR"
                     /locus_tag="ACHK3A_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO8741538.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            6654..7253
                     /gene="yiiQ"
                     /locus_tag="ACHK3A_003501"
     CDS             6654..7253
                     /gene="yiiQ"
                     /locus_tag="ACHK3A_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO8741539.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQALLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            7361..8128
                     /gene="tpiA"
                     /locus_tag="ACHK3A_003502"
     CDS             7361..8128
                     /gene="tpiA"
                     /locus_tag="ACHK3A_003502"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO8741540.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(8183..8938)
                     /gene="cdh"
                     /locus_tag="ACHK3A_003503"
     CDS             complement(8183..8938)
                     /gene="cdh"
                     /locus_tag="ACHK3A_003503"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO8741541.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(9044..10033)
                     /gene="sbp"
                     /locus_tag="ACHK3A_003504"
     CDS             complement(9044..10033)
                     /gene="sbp"
                     /locus_tag="ACHK3A_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO8741542.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKVFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(10353..11315)
                     /gene="pfkA"
                     /locus_tag="ACHK3A_003505"
     CDS             complement(10353..11315)
                     /gene="pfkA"
                     /locus_tag="ACHK3A_003505"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO8741543.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(11496..12398)
                     /gene="fieF"
                     /locus_tag="ACHK3A_003506"
     CDS             complement(11496..12398)
                     /gene="fieF"
                     /locus_tag="ACHK3A_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO8741544.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(12547..13047)
                     /gene="cpxP"
                     /locus_tag="ACHK3A_003507"
     CDS             complement(12547..13047)
                     /gene="cpxP"
                     /locus_tag="ACHK3A_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO8741545.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            13197..13895
                     /gene="cpxR"
                     /locus_tag="ACHK3A_003508"
     CDS             13197..13895
                     /gene="cpxR"
                     /locus_tag="ACHK3A_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO8741546.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            13892..15265
                     /gene="cpxA"
                     /locus_tag="ACHK3A_003509"
     CDS             13892..15265
                     /gene="cpxA"
                     /locus_tag="ACHK3A_003509"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO8741547.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(15371..16045)
                     /gene="yiiM"
                     /locus_tag="ACHK3A_003510"
     CDS             complement(15371..16045)
                     /gene="yiiM"
                     /locus_tag="ACHK3A_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO8741548.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGVSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(16194..17177)
                     /gene="kdgT"
                     /locus_tag="ACHK3A_003511"
     CDS             complement(16194..17177)
                     /gene="kdgT"
                     /locus_tag="ACHK3A_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO8741549.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
BASE COUNT         4316 a         4493 c         4292 g         4169 t
ORIGIN      
        1 gaaggtgcct tttttctgcg ttacctgttg gcctacacag taaagaaatt acgcgaaaga
       61 tgaagcgtaa tcagacctct tccatgcgac ccagcagggc ctgcagacgt tcctgccagc
      121 cgttctgctg ttctttcaga tggttgttct cacgctccag ctcttcgcgc tgatgctggg
      181 cattttgaac ttcctgcgac agtgagttgt ttttttcttt cagctcttcg atttccatct
      241 gcaacagagt gatggtatca atcgcctgct gtacttttgc ttccagtttc tcaaacactt
      301 ctaatgacat tgtcatacct ctcctgaatt gcaaggcgtt gatggataaa aaatcctcgt
      361 cccgattacc ggtgacgcct taataaatac gagcgcactt tagttagctc cgattgtatg
      421 aagccgcgcc atcgctgtcc agcggcacgc cttgcagatt acggtttgcc acacttttca
      481 tccttctcct ggagacataa tccacaccaa tcgaaaatgt taataaattt gttgcgcgaa
      541 tgatctaaca aacatgcatc atgtacaatc agatggaata aatggcgcga taacgctcat
      601 tttatgacga agcacacaca ttttaagttc gatatttctc gtttttgctc gttaacgata
      661 agtttacagc atgcctacaa gcatcgtgga ggtccgtgac tttcacgcat acaacaaaca
      721 ttaactcttc aggatccgat tatgagtcaa acatcaacct tgaaaggcca gtgcattgct
      781 gaattcctcg gtaccgggtt gttgattttt ttcggtgtgg gttgcgttgc agcactaaaa
      841 gtcgctggtg cgtcttttgg tcagtgggaa atcagtgtca tttggggact gggggtggca
      901 atggccatct acctgaccgc aggggtttcc ggcgcgcatc ttaaccccgc tgttaccatt
      961 gcattgtggc tgtttgcctg tttcgacaag cgcaaagtta tcccttttat cgtttcacaa
     1021 gttgccggcg ctttctgtgc tgcggcttta gtttacgggc tttactacaa tttatttttc
     1081 gacttcgagc agactcatca tattgttcgc ggcagtgttg aaagtgttga tctggctggc
     1141 actttctcta cttaccctaa tcctcatatc aattttgtgc aggctttcgc agttgagatg
     1201 gtgattaccg ctattctgat ggggctgatc ctggcgttaa cggacgatgg caacggtgta
     1261 ccacgcggcc ctttggctcc cttgctgatt ggtctactga ttgcggtcat tggcgcatct
     1321 atgggcccat tgacgggttt tgccatgaac ccagcgcgtg acttcggtcc gaaagtcttt
     1381 gcctggctgg cgggctgggg caatgtcgcc tttaccggcg gcagagacat tccttacttc
     1441 ctggtgccgc tttttggccc tatcgttggc gcgattgtag gtgcatttgc ctaccgcaaa
     1501 ctgattggtc gccatttgcc ttgcgatatc tgtgttgtgg aagaaaagga gaccacaact
     1561 ccttcagaac aaaaagcttc gctgtaatat gactacggga caattaaaca tgactgaaaa
     1621 aaaatatatc gttgcgctcg accagggcac caccagctcc cgcgcggtcg taatggatca
     1681 cgatgccaat atcattagcg tgtcgcagcg cgaatttgag caaatctacc caaaaccagg
     1741 ctgggtagaa cacgacccaa tggaaatctg ggccacccaa agctccacgc tggtagaagt
     1801 gctggcgaaa gccgatatca gttccgatca aattgcagct atcggtatta cgaaccagcg
     1861 tgaaaccact attgtctggg aaaaagaaac cggcaagcct atctataacg ccattgtctg
     1921 gcagtgccgt cgtaccgcag aaatctgcga gcatttaaaa cgtgacggtt tagaagatta
     1981 tatccgcagc aataccggtc tggtgattga cccgtacttc tctggcacca aagtgaagtg
     2041 gatcctcgac catgtggaag gctctcgcga gcgtgcgcgt cgtggtgaat tgctgtttgg
     2101 tacggttgat acgtggctta tctggaaaat gactcagggc cgtgtccatg tgaccgatta
     2161 caccaacgcc tctcgtacca tgttgttcaa catccatacc ctggactggg acgacaaaat
     2221 gctggaagtg ctggatattc cgcgcgagat gctgccagaa gtgcgtcgtt cttccgaagt
     2281 gtacggtcag actaacattg gcggcaaagg cggcacgcgt attccaatct ccgggatcgc
     2341 cggtgaccag caggcggcgc tgtttggtca gttgtgcgtg aaagaaggaa tggcgaagaa
     2401 cacctatggc actggctgct ttatgctgat gaacactggc gagaaagcgg tgaaatcaga
     2461 aaacggcctg ctgaccacca tcgcctgcgg cccgactggc gaagtaaact atgcgctgga
     2521 aggtgcggtg tttatggcgg gcgcgtccat tcagtggctg cgcgatgaga tgaagttgat
     2581 taacgacgcc tacgattccg aatatttcgc caccaaagtg caaaacacca atggtgtgta
     2641 tgtggttccg gcctttaccg ggctgggtgc gccatactgg gatccgtatg cacgcggggc
     2701 gattttcggt ctgactcgtg gggtgaacgc taaccacatt atacgcgcga cgctggagtc
     2761 tattgcttat cagacgcgtg acgtgctgga agcaatgcag gccgactccg gtattcgtct
     2821 gcacgcgctg cgcgtggacg gcggtgcggt ggcgaacaat ttcctgatgc agttccagtc
     2881 cgatattctc ggtacccgcg ttgagcgccc ggaagtgcgc gaagtcaccg ctttgggtgc
     2941 ggcctatctt gctggtctgg cggttggctt ctggcagaat cttgacgagc tgcaagagaa
     3001 agcggtgatt gaacgcgagt tccgtccagg catcgaaacc actgagcgta attaccgtta
     3061 cgcaggctgg aaaaaagcgg tcaaacgcgc gatggcatgg gaagaacacg acgagtaata
     3121 atgattgcct gatgcgacgc ttgcgcatct tatcaggcct acgagtcgga tacacatccg
     3181 gcatcacgca gactctgaaa ccgtgcgggg gcaaccccgc acacatcaat aatcccgctc
     3241 ttcccctgtg ctacacttcg cgccattcct tactgcttag agtttgctat gagacgagaa
     3301 cttgccatcg aattttcccg cgtcaccgaa tcagcggcgc tggctggcta caaatggtta
     3361 ggacgcggcg ataaaaacac cgcggacggc gcggcggtaa acgccatgcg tattatgctc
     3421 aaccaggtca acattgacgg caccatcgtc attggtgaag gtgaaatcga cgaagcaccg
     3481 atgctctaca ttggtgaaaa agtcggtact ggtcgcggcg acgcggtaga tattgctgtt
     3541 gatccgattg aaggcacgcg catgacggcg atgggccagg ctaacgcgct ggcggtgctg
     3601 gcagtaggcg ataaaggctg tttcctcaac gcgccggata tgtatatgga gaagctgatc
     3661 gtcggaccgg gagccaaagg caccattgat ctgaacctgc cgctggcgga taacctgcgc
     3721 aatgttgcgg cggcgctcgg caaaccgttg agcgaactga cggtaacgat tctggcaaaa
     3781 ccgcgccacg atgccgttat cgcagaaatg cagcaactcg gcgtgcgcgt atttgctatt
     3841 ccggacggcg acgttgcagc ctcaattctc acctgtatgc cagacagcga agttgacgtg
     3901 ctgtacggta ttggtggcgc gccggaaggc gtggtttctg cggcggtgat ccgcgcatta
     3961 gatggtgaca tgaacggtcg tctgctggcg cgtcatgacg tcaaaggcga caacgaagag
     4021 aatcgtcgca ttggcgagca ggagctggca cgctgcaaag cgatgggcat cgaagccggt
     4081 aaagtattgc gtttgggcga tatggcgcgc agcgacaacg tcatcttttc tgccaccggc
     4141 atcaccaaag gtgatctgct ggaaggtatt agccgcaaag gcaatatcgc caccaccgaa
     4201 acgctgctga tccgtggtaa atcacgcacc attcgtcgca ttcagtccat ccactatctg
     4261 gatcgtaaag acccggaaat gcaggtgcac atcctctgat tgatttgatc gattgagcct
     4321 tccagtcctt cgggactgga atttttttgt tcggagaacg aagataaggc aagtcaatca
     4381 aaacaggaga aaaacatggc tgattgggta acaggcaaag tcactaaagt gcagaactgg
     4441 accgacgccc tgtttagtct caccgttcac gctcccgtgc ttccgtttac cgccgggcaa
     4501 ttcaccaaac ttggccttga aattgaaggc gaacgcgttc agcgcgccta ctcctatgtt
     4561 aactcgcccg ataatccgga tctggagttt tacctggtca ccgtccccga tggcaaatta
     4621 agtccacgac tggcggcact gaaaccgggc gatgaagtgc aggtggttag cgaagccgct
     4681 ggcttctttg tgctggatga agtaccagat tgcgaaacgc tatggatgct ggcaaccggt
     4741 acagcgattg gcccttattt atcgattctg caattgggca aagatttaga acgcttcaaa
     4801 aacatggtgc tggttcacgc cgcacgatat gccgccgact taagctatct acctctgatg
     4861 caggaactgg aaaaacgcta cgaggggaaa ctgcgcattc agacggtggt cagtcgggaa
     4921 acggctgcgg gttcgctcac tgggcgatta ccggcgttaa ttgaaagtgg tgaactggaa
     4981 agcgcggttg gcctgccgat gaataaagaa accagccatg tgatgctgtg cggcaatcca
     5041 cagatggtgc gcgatacaca acagttgctg aaggagaccc ggcagatgac gaaacattta
     5101 cgtcgccgac cgggccatat gacagcggag cattactggt aagcggttac ttgtcgataa
     5161 acggcacgat caagagatcc gcactcaact tattgatcat cccgcgatat gccggcatca
     5221 aacggttgat aaatgagtga tgatgaccac agacaaggag gtcgcactgc tctttttgca
     5281 taatttccag cagcgtttcc ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc
     5341 attgaatatt tttcgtcagt ttatacagct tgttatccga cttattcttc aacaattgaa
     5401 gaatatcttc tgttgcaggg aagtagatac ccggatacaa ctcgcttaag ccatcatcaa
     5461 tatgaattaa cgtcaggtga gcgtcattat gtctggcgag ctccagggct ttattcacca
     5521 gtaaggcatc ttcttcattc ccggaaattg ccacgccaat gtgtttataa gccatgttta
     5581 actccttcta aagcaactcc atcaagctaa cgaacgcagt gataactcaa aaataatcat
     5641 ctcggcctgg catgagaagg tgaaggccgc atcaagctca acttgcccct ccagatcctt
     5701 aatcttatag gccacgctgg ccccttcatc tgcagaggat gcagcggcgg caagctcttt
     5761 cagtttggtc ataaaagact ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact
     5821 ggtacagttg tcgttatcaa tcaccgtacc gatatcaatc gcacatcctt tagtactgca
     5881 tttatctacg acatctttca tagggcacct ctgtatattc gcctctctgt cgattacgac
     5941 ttaagaataa tgcgccgtta aaggaagaac aaacaagtaa tgatcttatg gattaccatt
     6001 ttttttgata ttgcttaagg tgaagagttc agaaggattt tccgggcatt gtcacttgca
     6061 tcgtggaaat aacaatcccg gaaaaaaata gcgataagag atatattaaa ggttaattac
     6121 ttgatttatt gtcggcttta tacttcacat cctgggtatc tttaccatat ttattttcgc
     6181 cttgtgtgcc aacaaacgca ccaagatcga taagcatcat caccagaatc aatgtcggga
     6241 caaaacgccc caccgcccat tgccagacac ccggtaaaat tgcccagtta cccgccagca
     6301 gcatccacgc cacaatcatc agaaatgccc atgcgccgga acgcccgcga tcatgcaagc
     6361 gcttaacggt tactgccgct gttggccaaa gcaagcacac aaggcaaaac gccgcggtct
     6421 gaatatcgag taaattttta cccgccagtg agaaaagcac cagcatgcct gcgaaccaca
     6481 ggcctatcca aatccagaaa tcacggcgtc caatacgccc tttaaatgag aataaccatt
     6541 gctgtatggt catgtaagtt ccttgatggt tgtcttttcc aggattctac ccttttgaca
     6601 agggcgacag atatcgtttt aatcggagcc agtcataaca aaaggtactg tcaatgaagc
     6661 cagggtgtac gctgtttttt ctcttatgtt ctgcattaac cgttacaaca acggcgcatg
     6721 cacaaacacc agatacggca acgaccgcgc cttatctgct ggctggagcc cctactttcg
     6781 atctctccat cagccagttt cgagaagact ttaacagcca gaatcccagc ctgccactga
     6841 acgaatttcg tgccatcgac agcagtcccg acaaagccaa tctcactcgt gcagcaagta
     6901 aaattaacga gaacttgtat gcttctactg cgctggagcg cggtacctta aaaatcaaaa
     6961 gcattcaaat gacctggcta cccatccagg ggccagagca aaaagccgcg aaagcgaaag
     7021 ctcaggcata catggcagcg gtgatccgca cactcacccc attaatgacc aaaactcaaa
     7081 gccagaaaaa actgcaggcg ctactaacgg cggggaaaaa caaacgttat tacaccgaga
     7141 cagaaggtgc actgcgttat gttgtcgcgg acaacggcga aaaggggctg accttcgctg
     7201 ttgaaccgat taagctggcg ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag
     7261 caaagccttt gtgccgatga atctctatac tgtttcacag acctgctgcc ctgcggggcg
     7321 gccatcttcc tttattcgct tataagcgtg gagaattaaa atgcgacatc ctttagtgat
     7381 gggtaactgg aaactgaacg gcagccgcca catggttcac gagctggtat ctaacctgcg
     7441 taaagagctg gcaggtgttg ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat
     7501 cgatatggcg aagcgtgaag ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga
     7561 cctgaacctg tccggcgcat tcaccggtga aacctctgct gctatgctga aagacatcgg
     7621 cgcacagtac atcatcatcg gccactctga acgtcgtact taccacaaag agtctgacga
     7681 actgatcgcg aaaaaattcg cggtgctgaa agagcagggt ctgactccgg ttctgtgcat
     7741 cggtgaaacc gaagctgaaa acgaagcggg caaaactgaa gaagtttgtg cacgtcagat
     7801 cgacgcggta ctgaaaactc agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga
     7861 acccgtatgg gcaatcggta ctggcaaatc tgcaactccg gctcaggcac aggctgttca
     7921 caaattcatc cgtgaccaca tcgctaaagt tgacgctaac atcgctgaac aagtgatcat
     7981 tcagtacggc ggctctgtaa acgcgtctaa cgctgcagaa ctgtttgctc aaccagacat
     8041 cgacggcgcg ctggttggcg gtgcttctct gaaagctgac gctttcgcag tgatcgttaa
     8101 agctgcagaa gcggctaaac aggcttaagt ctgacaggtg ccggatttca tatccggcac
     8161 ttactttcct taactcctcg ccttaacgca aaatctcaca ctgatgatcc tggatttcct
     8221 cggctgaagc acggttaagc gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt
     8281 tatcactctg ccgcaccatc gccagcccgt agcttcccat atgttcccgc gcctcaggca
     8341 cttcttctgc aagcatcata aatgggctgc gttgtaccag ttcgctttcc gttacccgac
     8401 gcgccaggta ttcatgcccg cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt
     8461 tcgccagatt gttatccagc tgttcgcgca catcaggacg aatacaagag atatgaatat
     8521 gaaaatggtt ttgcgtacgc ccggtgcgag agttgatcgc caaagaaacc gcgcgatcgg
     8581 gaaccggctg gccgtatttt ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga
     8641 agttcggcgt tgaaggatcg gtcaacaaag gactttcagt accgttaata cgatacgttg
     8701 gcatcaacag atattgcagt gggccattaa gatcttttaa aaccacgtat ccggcattgg
     8761 gtttgacttc cgcacatggc gaaggatttt gattttgctg ctgattgggc aaacattcct
     8821 caaggacaat cttacgtaat gtatccgact cttcaccggt taatttccag taaccaatac
     8881 cggcagccac aacggcgata actatcatca ccaaaaaaag aagacccgct tttttcatct
     8941 tttttccctg tacctcaaag agatgcaagg gtaacgcaaa atcgtgacaa ataaaaaacc
     9001 cagtcgcgag aaaccaccgg gtttgataat tatcctggag aaatcagcgt ttgctaatct
     9061 gatcgaacgt accgccgtta gcaaaatgct ctttttgcgc tttcgtccag ccgccgaact
     9121 cttcatcaat ggtgaataac ttcagctttg gaaacgcatt ttcgtacttt ttcgccacct
     9181 cagcatcgcg cggacggtag tagtttttcg cggcaatttc ctgaccttct ggcgagtaga
     9241 gatatttcag gtaggcttcc gccacctctt tggtgccttt tttctcgacc actttatcga
     9301 ccaccgacac ggttggctct gcgaggatag actcactcgg cgtgacaatt tcgaatttat
     9361 ctttccccag ttcattcgct gccagcagag cttcgttttc ccaggcaatc agtacatcgc
     9421 caatcccgcg ctcgacaaag gtattagtgg agccgcgcgc gccagaatcc agaacttcga
     9481 cgtttttata cagtgcccga acaaaatcct gtgcttttgc ctgatcgttg ttgttgtgat
     9541 gcagcgcgta gccccaggct gccaggtagt tccagcgcgc gccaccagag cttttcggat
     9601 taggcgtgat caccgaaaca cccggtttaa tcagatcgtt ccagtcatgg atctgcttcg
     9661 gattgccctt acgcaccagg aaaacaatgg tggaagtgta cggtgcggag ttatccggca
     9721 gacgtttgat ccactcttta tcaatccgcc cgcgttccgc aatcgcgtcc acgtcatagg
     9781 ccagagccag cgtgacaaca tcagcttcaa taccgttgat taccgacgtc gcttgtttgc
     9841 ctgaaccacc gtgggactga cggatcacca cgttatcgcc agtttgctgt ttccagtggg
     9901 cgctgaatac cttgttgtac tgttcgtaca attcgcgcgt tggatcatat gaaacgttaa
     9961 gaagctgaat atcctttgcc ataacgctgg ttgccgccag caaaaatgtt aaccctacgc
    10021 cccacttgtt catcgcccga ctctcttatg ttgtgttgtg atgagcaaag cgtgccagaa
    10081 ggttaaccaa acattaaaga ataaaaaaag attggctata acttgcgggt atatgtaggg
    10141 ggattaaaaa ggcggaagaa atccgcctca tattgctgac aaagtgcgct ttgtccatgc
    10201 cggatgcggc gcgaacgcct tatccggcct acaaaagttc gcaaattcaa taaattgcag
    10261 aattcatgta ggcctgataa gcgaagcgca tcaggcattt ttgcttctgt catcggtttc
    10321 aggctaaagg aatctgcctt tttccgaaat cattaataca gttttttcgc gcagtccagc
    10381 cagtcgcctt tgaacggacg cttcatgttt tcgatagcgt cgatgatgtc gtggtgaacc
    10441 agctgttcgt tctggatacc tacgcaacga ccaccgtaac ctgccagcag cagctcgata
    10501 gcgtaagcgc ccatacggga agccagaata cggtcgtaag gcaccggaga accaccgcgc
    10561 tggatgtggc ccagcacagt tgcgcgggtt tcacgaccgg tttctttctc gatgaaatgc
    10621 gccagttcgt caacatcaca catatgttcg gtaatcgcca ctatcgcgtg ttttttacct
    10681 ttcgcgatac ccgctttgat ttcgtttacc aggtcttcac ggctgaattc aacttccgga
    10741 accacaacga attcacagcc accggcaata gccgcagcca aagtcagatc gccacaataa
    10801 cggcccatca cttccaccac ggaaatacgc tggtgagaag aagaggtgtc acgcagacgg
    10861 tcgatcgctt ctacaacggt gctcagcgca gtgaagaaac cgatagtgta gtcagtgcct
    10921 ttgatgtcgt tgtcgatagt gcccggcaga ccgatgcacg ggaagcccat ttcggtcaga
    10981 cgcattgcac ccatgtagga accgtcaccg ccgataacca ccagcgcgtc gataccacgt
    11041 tttttcaggt tttcgatagc cacggcgcgg atgttctcgt cgcggaattc cgggaaacgc
    11101 gcagaaccga ggaacgtacc accacggttg atcatgtcag aaacgctgta acggtctagc
    11161 tgtaccatac ggtcttcata cagacccaga tagccgtcat aaatgcccat tacttccaga
    11221 ccttctgtca gcgcagaacg aacaaccccg cgaattgcgg cgttcatgcc tggcgcatca
    11281 ccgccgcttg tcaacacacc gattttctta atcatgacta cctctgaact ttggaatgca
    11341 aaatgaaatc tgttgccgga agtcttcttg cacatcgaag tgatccaacg aatgtgcaaa
    11401 tagtataaca atcacttcct gctgaattga ttcaggtcag gccaaatggc ggtattttat
    11461 acacaaaatg cgggtctggc tctcttttat actgattatg aaagcataga ccgtttaccc
    11521 tccctgggta cgacggaaca ggggtcctga tggataatca catccgatcc cggaaaacgc
    11581 cgtaaaatag cctgctctac ctgatccgcc accatatgtg cctgaaccaa aggcagagag
    11641 tcttccattt ccaaatgaat ctgaataaag cgggtcggcc ctgactgccg cgtgcgaaga
    11701 tcgtgagcgc cgctaacacc cggccaggaa gtcacgatat caataatttc ttgccgttct
    11761 tcatcaggca atgcgcgatc cagtaatgac tgtaccgcct catatcccat gcgtaacgcg
    11821 ctatataaaa tatagatgcc gattcccaat gcaaacagag catcggcgcg atgccagccg
    11881 taccaggaca accccagcgc cagcagaatt gcaccgttca tcataacatc agactggtaa
    11941 tgtagcatat cagcccgcac cgcctggctt tgcgtccggc gcaccaccca acgctgaaac
    12001 gagacaagga taatcgtaca aattagcgcc acaattgtca cgataacccc gacgcctgga
    12061 tctgtcatcg gtgttggaga tatcagatgt tgaatacccg tcaaaaacag gaatagtgct
    12121 gaaccggaga taaacatact ttgcgccagc gccgcgaggg actcagcttt accgtgacca
    12181 aacgagtgat tatcgtcggc aggttgcagg gaatatcgca ccaccagtaa attcgtcaac
    12241 gacgcgccga tatccaccag cgaatccacc agcgcggcga gaatactcac cgacccggta
    12301 taccaccatg caaaaatttt aatcagcaat agcagtgaag ccatcgccgt tgcagcaatc
    12361 gccgcccgac tgaccagccg tccataagat tgattcataa atactcccgc tatcaactga
    12421 cgctagtata acggaagcaa atcatctgca atgcattaag cagcaggcaa attgaggata
    12481 aaaaaaaccc ccacatcatg tgggggaaga cagggatggt gtctatggca aggaaaacag
    12541 ggtttactac tgggaacgtg agttgctact actcaatagc ttcaacgatg aacttttttg
    12601 ccattgcgtc acgtcacgca actgctccat tcgttgttga tgtttctcat ttaaaaccgc
    12661 ttgctgctcc ggcgttaaca ggcgatacat ttggttgcgg acttttgcca tctcaacctg
    12721 acgagcaatt tgctcattcg ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa
    12781 attttctgcg gtgacaaggc gatgcattgt ctccagttcg ctaacattaa caggaggctg
    12841 ttcgtgccgg gcctgttgca taagatctcg catctgctga cgctgatgtt cggttaaact
    12901 tatgccgtcg aacatatggc tctgcgtact gcgctgcgta agttcttcac ccggatgcca
    12961 gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca gcgttgaggc
    13021 catgacggca gcggtaacta tgcgcatcat ttgctcccaa aatctttctg tcgcgattca
    13081 acgatagaga gtttacgatt caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa
    13141 agtcatggat tagcgacgtc tgatgacgta atttctgcct cggaggtatt taaacaatga
    13201 ataaaatcct gttagttgat gatgaccgag agctgacttc cctattaaag gagctgctcg
    13261 agatggaagg cttcaacgtg attgttgccc acgatgggga acaggcgctt gatcttctgg
    13321 acgacagcat tgatttactt ttgcttgatg taatgatgcc gaagaaaaat ggtatcgaca
    13381 cattaaaagc acttcgccag acacaccaga cgcctgtcat tatgttgacg gcgcgcggca
    13441 gtgaacttga tcgcgttctc ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt
    13501 ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg cactggagcg
    13561 aacaacagca aaacaacgac aacggttcac cgacactgga agttgatgcc ttaagtctca
    13621 atcctggtcg ccaggaagcc accttcgacg ggcaaacact ggagttaacc ggtactgagt
    13681 ttaccctgct ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt gagcatttaa
    13741 gccaggaagt gctgggcaag cgcctgacgc ctttcgaccg cgctatcgat atgcatattt
    13801 ccaacctgcg tcgtaaactg ccggatcgta aggatggtca cccgtggttt aaaaccctgc
    13861 gtggtcgtgg ctatctgatg gtttctgctt catgataggc agcttaaccg cgcgcatctt
    13921 cgccatcttc tggctgacgc tggcgctggt gttgatgttg gttttgatgt tacccaagct
    13981 cgattcacgc cagatgaccg agcttctgga tagcgaacag cgtcagggtc tgatgattga
    14041 gcagcatgtt gaagcggagc tggcgaacga tccgcccaac gatttaatgt ggtggcggcg
    14101 tctgttccgg gcgattgata agtgggcacc gccaggacag cgtttgttat tggtgaccac
    14161 cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta actttattgg
    14221 tcaggccgat aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg
    14281 tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt tatctgattc gtccggccag
    14341 cagttctcaa tccgatttca taaacttact gtttgaccgc ccgctattac tgctgattgt
    14401 caccatgctg gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg caaaaccggc
    14461 gcgtaagctg aaaaacgctg ccgatgaagt tgcccaagga aacttacgcc agcatccgga
    14521 actggaagcg gggccacagg aattccttgc cgccggtgcc agttttaacc agatggtcac
    14581 cgcgctggag cgcatgatga cctctcagca acgtctactt tctgatatct ctcacgaact
    14641 gcgcaccccg ctgacgcgtc tgcaactggg tacggcgtta ctgcgccgtc gtagcggtga
    14701 aagcaaggaa ctggagcgta ttgaaaccga agcgcaacgt ctggacagca tgatcaacga
    14761 tctgttggtg atgtcacgta atcagcagaa aaatgcgctg gttagcgaga ccatcaaagc
    14821 caaccagttg tggagtgaag tgctggataa cgcggcgttc gaagccgagc agatgggcaa
    14881 gtcgttgacg gttaacttcc cgcctgggcc gtggccgctg tacggcaacc cgaacgccct
    14941 ggagagtgcg ctggaaaaca ttgttcgtaa tgccctgcgt tattcccata cgaagattga
    15001 agtgggcttt gcggtagata aagacggtat caccattacg gtggacgacg atggtcctgg
    15061 cgttagcccg gaagatcgcg aacagatttt ccgtccgttc tatcgtaccg atgaagcacg
    15121 cgatcgtgaa tctggcggta caggtttggg gctggcgatt gtagaaaccg ccattcagca
    15181 gcatcgtggc tgggtgaagg cagaagacag cccgctgggc ggtttacggc tggtgatttg
    15241 gttgccgctg tataagcgga gttaaactcc gcatttgtag gcaggataag gcgtttacgc
    15301 cgcatccggc atttgagcag gatgcctgat gcgacgctga tagcgtctta tcaggcctac
    15361 actccgggga ttattttccc cacaatcggc gggaaaaatc ttcgatcttg ccgctcagtc
    15421 ggcgcttttg catcgttcgc gtccagcttt tcgatacccc tgcggcggag agtaaacggt
    15481 gatactgatc gtcatcaaac ggcatatgcc atgcgatggc ggcagcctcc tgcacggtca
    15541 catcgctgac acgggaaacc aactccagtg gcgcatcagc agataccaac cctggtgcaa
    15601 ttacgctata caaccacccc actttacccg tgttttgcat caactgcgct atatcgctga
    15661 tatcaaaatg gtaattgagt ttatagcaag gtgagcgtgg ctggctgacc tgaattaatg
    15721 cctctcccca gcggaaaata tcgccaatat aaacattact ttccgtcaga ccgtcggttg
    15781 agagattttc gccaaacgca ggcgcaacaa acaactccgc ctgttccgga aattcccgcg
    15841 cccagtaaag ataatgctca cgaggataat gacacagcgc tctgtctggc ccgccgtgaa
    15901 cctttttctc cgcctgctcg tcaccttcca gccccaggtc cgtcagcatc aactcaccat
    15961 caacctggat tttggcaatt gcgctgggtt tgccttcggg ataagcctga atcttgcctg
    16021 tgtatacatc aaccggatat cgcatctgtt ccctctccac gagaaatttc cccattagac
    16081 catatttatt ttatgtcagg cgtgctgttt tcgcggtaag tatccaggca ggaactcaat
    16141 agcaaaccgg acgaacgtta gttcatccgg ctgtggggat taaggaagca ggtttatttc
    16201 accgtaccta aaatttcgat tttcgctgct ctggctttga ctttacgtga ccagatagag
    16261 gtaagaattg gcaccagaat cgaagtcaca atgaccgccg tcgcgaccag cgaagttgct
    16321 gccggagcca tcggtttaaa cgcaggcacc atttctgcaa tcagcacagg tgtcgctacc
    16381 gcggcccctg cggaactgga agcggcaatt ccggcagtgc catcgccacc gccaatcaat
    16441 ttatcagcga taatcaacgg aataccggtc acgataatta ctgccacacc caacaggatc
    16501 cccagcaaac ctgtctgggc aattaccgtc aaatcaatgg tattacccag tgcgaaggca
    16561 aagaatggaa tcagcgtctg caccgctttg ctgaaaaatt ctcgtaattc agggtcaagg
    16621 ttcccaaggg caaagcccac caggaacggt aatactgcgc cgacgaaaac atgcggttca
    16681 aacgaggcaa tcccggcagt gcccagaata atcatcgtca tgagcggccc ggactccaat
    16741 gacatcaaca caaatgcccc agcttcttct tttgtgccgt actgctgcat gatggaagcg
    16801 taaagtccgc cgttggtcat atccatcgcc gccaccagcg ccagcgttga aagtccggca
    16861 aagaatccaa cttcaacacc atgttccgga ataatgcgcg aggcaattgc cgcaaccacc
    16921 cacgcgacgg caatttttgt taccaccagc gtaccggatt tacgcaatac cgttcccgtc
    16981 gcgcttaatt ttattgacgc ccccatgcaa aaaaaccaca ccgccagaat gggcaccgta
    17041 ccggtaatca tcccgttggt gaatgatcca aaatatttcc ccgcctccgg cgagaaggtg
    17101 tggcacagtg cgccaaggaa taacgggacg agcatcatcc cccccgggat tttctcaatc
    17161 gagcgtttta tctgcatttc catcacctgt cggcatattt ttaagggctg atgcgtcatt
    17221 cctcaatgga gcatcagtgt tgatggaaac gaagatagac attcatttaa
//
LOCUS       DAZGHF010000102        16901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGHF010000102 DAZGHF010000000
VERSION     DAZGHF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.102"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(24..719)
                     /gene="araD"
                     /locus_tag="ACHK3A_003512"
     CDS             complement(24..719)
                     /gene="araD"
                     /locus_tag="ACHK3A_003512"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO8741550.1"
                     /translation="MLEDLKRQVLEANLALPKHNLITLTWGNVSAVDRERGVLVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(919..2421)
                     /gene="araA"
                     /locus_tag="ACHK3A_003513"
     CDS             complement(919..2421)
                     /gene="araA"
                     /locus_tag="ACHK3A_003513"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO8741551.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGIKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(2432..4132)
                     /gene="araB"
                     /locus_tag="ACHK3A_003514"
     CDS             complement(2432..4132)
                     /gene="araB"
                     /locus_tag="ACHK3A_003514"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO8741552.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIANKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            4471..5349
                     /gene="araC"
                     /locus_tag="ACHK3A_003515"
     CDS             4471..5349
                     /gene="araC"
                     /locus_tag="ACHK3A_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO8741553.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAAKLS"
     gene            5435..6199
                     /gene="yabI"
                     /locus_tag="ACHK3A_003516"
     CDS             5435..6199
                     /gene="yabI"
                     /locus_tag="ACHK3A_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO8741554.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLAPLISAVGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6313..7011)
                     /gene="thiQ"
                     /locus_tag="ACHK3A_003517"
     CDS             complement(6313..7011)
                     /gene="thiQ"
                     /locus_tag="ACHK3A_003517"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO8741555.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERSEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKASASAILGITG"
     gene            complement(6995..8605)
                     /gene="thiP"
                     /locus_tag="ACHK3A_003518"
     CDS             complement(6995..8605)
                     /gene="thiP"
                     /locus_tag="ACHK3A_003518"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO8741556.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            complement(8581..9564)
                     /gene="thiB"
                     /locus_tag="ACHK3A_003519"
     CDS             complement(8581..9564)
                     /gene="thiB"
                     /locus_tag="ACHK3A_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO8741557.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9595..9694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9728..11383)
                     /gene="sgrR"
                     /locus_tag="ACHK3A_003520"
     CDS             complement(9728..11383)
                     /gene="sgrR"
                     /locus_tag="ACHK3A_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO8741558.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAA
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQILPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     SSNQLKIQAFDDFFGYRALIDEVNVWVLPEIADDPAGGLMLKGPQDEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHTRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            11472..11603
                     /gene="sgrT"
                     /locus_tag="ACHK3A_003521"
     CDS             11472..11603
                     /gene="sgrT"
                     /locus_tag="ACHK3A_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO8741559.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(11711..12316)
                     /gene="leuD"
                     /locus_tag="ACHK3A_003522"
     CDS             complement(11711..12316)
                     /gene="leuD"
                     /locus_tag="ACHK3A_003522"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO8741560.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVQANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12327..13727)
                     /gene="leuC"
                     /locus_tag="ACHK3A_003523"
     CDS             complement(12327..13727)
                     /gene="leuC"
                     /locus_tag="ACHK3A_003523"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO8741561.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13730..14821)
                     /gene="leuB"
                     /locus_tag="ACHK3A_003524"
     CDS             complement(13730..14821)
                     /gene="leuB"
                     /locus_tag="ACHK3A_003524"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO8741562.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            complement(14821..16392)
                     /gene="leuA"
                     /locus_tag="ACHK3A_003525"
     CDS             complement(14821..16392)
                     /gene="leuA"
                     /locus_tag="ACHK3A_003525"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO8741563.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16485..16571)
                     /gene="leuL"
                     /locus_tag="ACHK3A_003526"
     CDS             complement(16485..16571)
                     /gene="leuL"
                     /locus_tag="ACHK3A_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO8741564.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRLVSGIQN"
BASE COUNT         3730 a         4588 c         4596 g         3987 t
ORIGIN      
        1 ttggctgtgg ttttatacag tcattactgc ccgtaatatg ccttcgcacc atgcttacgc
       61 agatagtgtt tatccagcag cgtttgctgc atatccggta actgcggcgc taactgacgg
      121 cagaatatcc ccatataagc gacctcttcc agcacgatgg cgttatgcac cgcatcttcg
      181 gcatttttgc cccatgcaaa tgggccgtga gaatggacca ggacgccggg catttgtgct
      241 gcgtcgattc cctgtttttc gaaggtttct acgatgacgt taccggtttc ccactcatat
      301 tcaccgttga tttctgcgtc ggtcattttg cgggtgcagg gaatggtgcc gtagaaatag
      361 tcggcgtggg tggtaccggt tgccggaatt gactggcccg cctgcgccca gatggtggcg
      421 tggcgcgagt gtgtgtgcac aatgccgcca atggacggga atgcctgata gagcagccgg
      481 tgagttggcg tgtcggagga gggctttttc gttccttcaa ccacttcacc ggtttcgatg
      541 ctgaccacga ccatatcgtc agcggtcatg acgttgtaat cgacgccgga aggtttgatc
      601 accaagacgc cgcgctcgcg atcaacggcg ctgacattgc cccatgtgag cgtgatcagg
      661 ttatgttttg gcagcgccaa attggcttct aatacctggc gtttgagatc ttctaacatg
      721 ttgactcctt cgtgccggat gcgctttgct tatccggcct acaaaatcgc agcgtgtagg
      781 cctgataaga cgcgtcagcg tcgcatcagg cggtgaatgc cggatgcgct ttgcttatcc
      841 ggcctacaaa atcacagcgt gtaggcctga taagacgcgt cagcgtcgca tcaggcgtta
      901 cacacctgat gcggctactt agcgacgaaa cccgtaatac acttcgttcc agcgcagcgc
      961 gtctttaaac gctggcaggc gggtatcgtt atcaatcacc gtgatttcaa tgtcgtgcat
     1021 ctcggcgaac tggcgcatat cgttgaggtt cagcgcatgg ctgaaaacgg tatggtgcgc
     1081 gccaccagca aggatccacg cttcggaagc agttggcaga tccggttgcg ctttccacag
     1141 cgcattcgcc accggcagtt tcggcaggga gtgcggtgtt ttcaccgtgt cgatgcagtt
     1201 aaccagcaga cggtaacgat cgccgagatc aatcaggctg gcgacaatcg ctggaccggt
     1261 ttgggtattg aagatcaggc gggcaggatc gtctttaccg ccaataccga ggtgctgaac
     1321 gtcgaggatc ggtttctctt ccacagcgat cgacggacag acttccagca tgtgggagcc
     1381 gagcaccagg tcattgcctt tctcgaagtg ataggtgtag tcctccataa aggaggtgcc
     1441 gccctgcaga ccggttgaca tcaccttcat gatgcgaagc agggcggcag ttttccagtc
     1501 gccttcgccc gcaaagccgt aaccctgctg catcagacgc tgtacggcca gaccaggaag
     1561 ctgtttcaga ccgtgcaaat cttcaaaggt ggtggtgaac gcgtggaagc caccttgttc
     1621 caggaaacgc ttaatcccca gctcaatacg cgccgcttcc agcacgttct ggcgtttttc
     1681 gccgtggatt tgtgtggcag gcgtcatggt gtagcagctt tcgtactcat cgaccagcgc
     1741 gttaacatcg ccatcgctga tggagttcac tacctgcacc agatcgccaa ccgcccaggt
     1801 attgacggag aaaccgaact tgatctgtgc ggcaacttta tcaccatcgg tgaccgccac
     1861 ttcacgcatg ttatcgccaa aacggcagac tttcagatga cgggtatcct gtttagagac
     1921 cgcctgacgc atccaggagc cgatacgctc atgggcttgt ttatcctgcc agtgaccggt
     1981 aacgacggca tgttgctgac gcatacgcgc gccaatgaag ccgaactcgc gaccgccatg
     2041 tgcagtctgg ttcaggttca taaagtccat atcgatgcta tcccacggca gcgccgcgtt
     2101 aaactgggtg tggaattgca gcaacggttt gttgagcata gtcaggccgt tgatccacat
     2161 tttggccggg gagaaagtgt gcagccacac taccaggcca gcgcaacgat cgtcgtaatt
     2221 cgcgtcacgg caaatagcgg tgatttcatc cggcgtggtg cccagcggtt tcagcaccag
     2281 tttgcagggc agtttcgctt ccgtattcag cgcattaacg acgtgctcgg catgttgggt
     2341 gacctgacgc agggtttccg ggccatacag atgctggctg ccaattacaa accacacttc
     2401 ataattatca aaaatcgtca ttgtcgtgtc cttatagagt cggaacggcc tgggcagcct
     2461 gtgccggggc ggaagttgga agatagtgtt gttcggcgct catcgcccat tgctgatagc
     2521 ggcgataaag ctgttcaaag cgttgtgcct gttcgctgcg cggttgcagg gttttctcta
     2581 ccgcactggc cattttttgc tgggctgagg ggatgtctgc gtgcactttc gcggcgacgg
     2641 cagcgaaaat cgccgcaccg agcgcacagc actggtcaga ggcaacaatt tgcagcgggc
     2701 gattcagcac gtcgcagcag gcctgcataa tgacctggtt tttccgcgcg atgccgccca
     2761 gcgccatcac gttattgacg gcgatcccct gatcggtaaa gcactccatg attgcgcgtg
     2821 cgccaaaggc ggtggcagca atcaatccgc cgaacagcag cggagcgtcg gtagcgaggt
     2881 tcagatcggt aatcacccct ttcaggcgtt ggttagcgtt tggcgtgcgg cggccgttaa
     2941 accagtcgag caccaccggc aggtgatcca gagacggatt tttggcccat gcttcggtca
     3001 gcgccggaag cagttgtttc tggctggcgt tgatttgctc tttcagttcc ggatgctggg
     3061 cggcaagctg ttccagcggc cagccgagta cgcgaccgaa ccaggcgtag atatcaccaa
     3121 acgccgattg gcctgcttcc agaccgataa atccaggcac cacgctgcca tcaacctgac
     3181 cgcaaatacc tttcactgcc cgctcgccaa cgctctgttt gttggcaatc agaatgtcgc
     3241 aggtggaagt accgataact tttaccagag cgttaggctg tgcgcctgcg ccaactgcgc
     3301 ccatatggca gtcaaacgcg ccgccggaaa tcaccacgct ttcaggtagg ccgagacgct
     3361 gcgcccattc cgggcataag gtgcccaccg gaatatcggc agtccaggtg tcagtgaata
     3421 gcggggaagg caaatggcga ttgaggatcg ggtccagctc atcaaagaaa ctggctggcg
     3481 gcaggccgcc ccagctttca tgccatagcg acttatgccc ggcgctgcaa cgtccgcgac
     3541 gaatatcctg cgggcgggtg gtaccggaaa gcagagctgg cacccagtcg cacagctcaa
     3601 tccacgatgc ggcagattgc gccacggcgc tgtcctggcg agtcacatgc agtatttttg
     3661 cccagaacca ttcgctggaa taaataccgc caatatagcg ggagtagtca acgttgcccg
     3721 gcgtgtgaca taagcgggtt atctcttccg cttcttcaac cgcggtgtgg tctttccaca
     3781 atacgaacat cgcgttcggg ttttcggcaa actccgggcg cagcgccagg acattaccgt
     3841 ccgcgtcaat cggtgcgggc gtcgagccgg ttgtgtcaac gccaatcccg accacagctg
     3901 cgcgctgctc ggcgctaagc tctgcaagca cggttttcag cgccgcttcc attgactcaa
     3961 tgtagtcacg cggatgatga cggaactggt tatttggggc atcacaaaat tgccctttct
     4021 gccaacgggg ataccactct acgctggtgg cgatctcttc accggtggcg cagtccaccg
     4081 ccaaagctcg cacagaatca ctgccaaaat cgaggccaat tgcaatcgcc atcgtttcac
     4141 tccatccaaa aaaacgggta tggagaaaca gtagagagtt gcgaaaaaaa gcgtcaggta
     4201 ggatccgcta atcttatgga taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa
     4261 taatcaatgt ggacttttct gccgtgatta tagacacttt tgttacgcgt ttttgtcatg
     4321 gctttggtcc cgctttgtta cagaatgctt ttaataagcg gggttaccgg ttggattagc
     4381 gagaagagcc agtaaaagac gcagtgacgg caatgtctga tgcaatatgg acaattggtt
     4441 tcttctctga atggcgggag tatgaaaagt atggctgaag cgcaaaatga tcccctgctg
     4501 ccgggatact cgtttaacgc ccatctggtg gcgggtttaa cgccgattga ggccaacggt
     4561 tatctcgatt tttttatcga ccgaccactg ggaatgaagg gttatattct caatctcacc
     4621 atacgcggcc agggggtggt gaaaaatcag ggacgagaat ttgtctgccg accgggtgat
     4681 attttgctgt tcccgccagg agagattcat cactacggtc gtcatccgga ggctcgcgaa
     4741 tggtatcacc agtgggttta ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg
     4801 ccgtcaatat ttgccaatac gggtttcttt cgcccggatg aagcgcacca gccgcatttc
     4861 agcgacctgt ttgggcaaat cattaacgcc gggcaagggg aagggcgcta ttcggagctg
     4921 ctggcgataa atctgcttga gcaattgtta ctgcggcgca tggaagcgat taacgagtcg
     4981 ctccatccac cgatggataa tcgggtacgc gaggcttgtc agtacatcag cgatcacctg
     5041 gcagacagca attttgatat cgccagcgtc gcacagcatg tttgcctgtc gccgtcgcgt
     5101 ctgtcgcatc tgttccgcca gcagttagga attagcgtct taagctggcg cgaggaccaa
     5161 cgcattagtc aggcgaagct gcttttgagc accacccgga tgcctatcgc caccgtcggt
     5221 cgcaatgttg gttttgacga tcaactctat ttctcgcgag tatttaaaaa atgcaccggg
     5281 gccagcccga gtgaattccg tgccggttgt gaagaaaaag tgaatgatgt agccgccaag
     5341 ttgtcataat tggtaacgaa tcagataatt gacggcttga cggagtagca tagggtttgc
     5401 agaatccctg cttcgtccat ttgacaggca cattatgcaa gcattgctgg aacactttat
     5461 tacccaatcc accgtgtatt cattgatggc ggtggtgttg gtagcctttc tggagtcgct
     5521 ggcgctggtc ggtttgattc tacccggtac ggtgctaatg gcgggactgg gggcgctgat
     5581 tggcagtggc gagttaagtt tctggcacgc ctggctggca gggatcgtcg gttgtctgct
     5641 gggcgactgg atctccttct ggctgggctg gcgttttaaa aagccgttgc atcgctggtc
     5701 atttctgaag aagaacaaag cattactcga taaaaccgaa catgcgctgc atcagcacag
     5761 catgttcacc attctggtcg gtcgctttgt tggcccgacg cgtccgctgg tgccaatggt
     5821 ggcgggaatg ctggatctgc cggtggcaaa attcattacg ccgaatatta tcggctgcct
     5881 gctgtggcca ccgttttact tcctgccagg gattctggcg ggtgcggcga tcgatattcc
     5941 agccggaatg cagagcggcg agtttaaatg gttgctgctg gcgacggcgg tatttttgtg
     6001 ggttggtggc tggctgtgct ggcggttatg gcgcagcggt aaagcgactg accgtttgag
     6061 tcattatttg tcgcgcggtc gtttgttgtg gctggcaccg ttaatttctg ccgtcggtgt
     6121 agtggcgctg gtggtgttaa ttcgccaccc gttgatgccg gtgtatatcg atattttgcg
     6181 taaagtggtt ggggtttagg ggatagcctt gtgcgggttg cctgatgcga cgcttgccgc
     6241 gtcttatcag gcctacaaaa cgcactgtcc gtaggtcgga taaggcgttc acgccgcatc
     6301 cgacagtgca tactaacccg taatccccaa tattgccgaa gcactcgcct taccgctcaa
     6361 caactcatcg gtcataccct gccaggcgat gcgcccgtcg gcgaccacca ccgagcgcgt
     6421 ggcgatccgc gccgcatctt ccacactgtg cgacaccatc aatagcgtca ttttttgctg
     6481 ttggcagctc gtgctcacca gcgtcaacat ctcctgacgt aacgccggat cgagcgcaga
     6541 gaacggttca tcgagcaata aaatcggctg ttcgcgcacc agacaacgcg ctaacgccac
     6601 gcgctgtcgc tgaccgccag aaagctcgcc cggtaagcgc gccattaaat tatcaatccc
     6661 catctgacgg gcaatcgcgt gcattttctc ctgttgtgcc gcgttcagct tcaatcctgg
     6721 attaagcccc agcccgatgt tctgcgcgac cgtcaggtga ctgaacaggt tgttttcctg
     6781 aaacagcatc gacaccggac ggcgtgacgg cggcgtcgtg gtgtgatcaa cgccgtcgat
     6841 agtcagcgaa ccgctggctg gcgtcagaaa accggcgatc aaattcagca gggtactttt
     6901 acccgcgccg cttggcccga ggatcgccac ctgctcgctg cgttccaccg ttaagctaaa
     6961 acgcatcggc aaatggtggt aaagccaggt gatatcagtc agttttaaca tttcgccccg
     7021 gtagtttttc aatcacggta aacagcagaa aacagagtag cagcagaatt aacgcggtga
     7081 ccgcgccgtc ctggctgcga taggagccaa tttgctggta gagataaaac ggcagggtgc
     7141 ggaaatcatc gttaccaaac aacgccacca cgccaaaatc accaatcgat agcacacaag
     7201 caaaggccag tgcctgcgcc agtgggcgtt taagggcgcg cagctccacc acttttaagc
     7261 gtgaccagcc ttcaatgccc agcgactgac acaacatact gtaacgcgcg gtgatatcgc
     7321 gcatcgggtt ttccagcact ttcagcgcat aagggatcgc cattaacgca ttggtgaaaa
     7381 tcacaatgcc gtcagcagat tgtggcaggc cgatagtgtt gttgagcagt aaaaagaagc
     7441 cggtagccag cacaatcccc ggcatcgcga ggatcaacat gccgctcatc tccagcgcct
     7501 gacccgccag cattttctgc cgcgcccgca gttcgcgact gctccatagc agcatcatgg
     7561 tcagcactac gcataatacg cctgccgcca gcgcaatacg caacgaggtc cacagcgcct
     7621 gccacagcac cggctgcgcc agcacttccg gcaactggcg atttatccca tcgacgagca
     7681 ccgccagtaa cggtggcagc aacagcaaca gcgccagcac aattaacacc gtgtcgcaaa
     7741 tgcggctatg cagacgatcg tccgggtcgc gccagccttg cagcagcgta gtgccgggcg
     7801 caatggcctt actcaatcgc tgactcaaca gcaccagccc aaggcaacac accatctgga
     7861 tcagcgccag catcgccgcg cgggcaggat cgtagtcgta actcagcgcc tgataaatag
     7921 ccagctcgat agtcgtcgct tgcggaccgc cgcccagcga caacacggtg gcgaagctgg
     7981 cgaaacagag cataaagata agcgcggcaa ccggcgggat ttgtcgccgt aaccacggcc
     8041 attcgacgaa gcggaaaaaa tgccagccgc gcatcccaag ctgggcggca agctgacgtt
     8101 gttccccggg aatgttttcc agtgcctgga gtaataagcg gctcgccatc ggcagattaa
     8161 aaaatacatg cgccagcaaa atgccttgca ggccgtaggg cgaaaacgtc cactccagac
     8221 caagcgattg ccagagcgat gccagccagc cctggcgacc atagacgcta agaatgccga
     8281 aaacagcaac cagcaccggg aggatcaagg tcatggcaca cagacgcaac agcgccagcc
     8341 gtcctggaaa gcgcctgcgg tagagggcgc gggcaaggaa tatcgcgggc acgacagaga
     8401 gcagcgccga gagaaacgcc tgccagaagg agaagcgcac cacatgccac aggtagctgt
     8461 cctgccagat tgccacccag tcaccctgcg gcgcgttcca ccacagggcg agaaacgcag
     8521 ccagtgcaac cgccaccacc agcgtggcgg cgcttattcc tggaattaac cagccgggaa
     8581 ttaacggctg acggcgcgtt gccattcgct aatccatgcc tgacgttgtg ccgccacttc
     8641 ggctggcgtg aactccagcg tggttgccgg tttggtcaat tgttcaaaac cggcaggcag
     8701 cgtgacgttt gccaccggat acatccagtt gccggttggg atcgcattct gaaaagccgg
     8761 agaaaccata aactggagaa atttttgcgc cagctccggc tgcttgctgg cagcggtgcg
     8821 ggcggcgact tccacctgca gatagtgacc ttcgctgaag tttgcggcgg cgtagttatc
     8881 tttcttctct tcgagaatgt gataagccgg agaggtggtg taactcagta ccagatcgct
     8941 ttcacctttt aaaaacaggc cgtaggcttc gctccagcct ttggtgaccg tgaccgtttt
     9001 cttcgccagt ttctgccagg cttgtggggc gtcatcgcca tagacttttt gcatccacaa
     9061 cagcaggccc agccccggcg tactggtgcg cggatcctga taaatcaccc gccagttttg
     9121 atcgctctca acaagctctt tcaggctttg cggcggattt ttcagtttgt tcttgtcata
     9181 gacgaaggcg aaatagccgt aatcaaacgg tacgaaagtg tcatttttcc agccgccggg
     9241 aacgttaacg gcatccgctg ccacgccgct tttggcaaat agtccggttt tactggcggc
     9301 gtctaacagg ttgttatcca gccccagcac cacatcggct ttactgtttt tgccttccat
     9361 ccgcagacgg ttgagcagcg aaacgccatc ttccagcgcc accagtttca gttcgcagtt
     9421 acagtcggct tcaaaagctt ttttgatctt tggaccaggc ccccagtcgg cggcgaagga
     9481 atcgtaggta taaacagtca gaacgggttt agcgaaaacg ggcgctgtgc acagcaacag
     9541 caaggggaga cattttttta acactttgca cctcaaaaaa gagtggcaaa ggacttgaga
     9601 aggagcctca aatcccttcg ccggcgttat ccggatcagg ttcgacgggt attttctcag
     9661 cgcacgcgtc tgcgtggcac cccgttgaga acggcgttag tgtagtgatt ttgttatcaa
     9721 ccagcaatca tggatccggc ggcgcaaacc acgctgattt aaaatcgaac cagccgaggg
     9781 tattcatacg caggccgcgc atactgcgtt gcccctgaat gatcagccag tggtggataa
     9841 gcggcaccat tgctttgctg gcgaccagtt gctggcacca gttcgccagg tttatttcgc
     9901 cattgcgcca gcgggcggcg tcggcttgcc agtcgatcgg aatacagtgt tgtagcagcg
     9961 gcacctcgca caggtgcgcg aatagcgaaa aatccagcgg cagcgtaaag ttggcgctgt
    10021 taagccagat atcgctctcg atctctcctt catgccactg atcgtagctg atctctttga
    10081 tttccagcgt gacctggtga cttgccagaa tctgctgcat gatcccggca atcaccctat
    10141 gctcactgtg atcctgataa aaggtcaggg tgaggctttc cagaccagcc ggtttttcgc
    10201 tctttatggt gcgggtatgg tgccaacggg gaagcagccc ataggccgga aaccacaact
    10261 gctggtactg ttcctcggcg aaataaacca gattggtcgg cgaaagcaca tagcttatcc
    10321 agtctctgac ttgctgattc gccccgcgat gggtacggct gtcgaacagt aaatagtaac
    10381 aaccttcttc aaggcggctt tcaatctctt tttcctcgtc ttgcggtcct ttcagcatca
    10441 ggcctcccgc ggggtcatca gcaatttccg gcaagaccca gacgttaact tcgtcgatta
    10501 atgcccgata accaaagaag tcgtcgaatg cctgaatttt cagttgattg ctgctgttgc
    10561 ggatcaccgc ataaggaccc gttccgatgg gatggctggc aaagttgctg agggtttccc
    10621 attcgcgcgg caggatcatc gccggaactt gccccagcag taacggtaac cagcggtccg
    10681 gttgcgtgag atggatatcc agcgtccagg gcgtcggcga cacaatgtca gcaatatgcg
    10741 aatagagcgg cagcgtattg attcgtttta aagaggcgat cacgtcgtcc atttccagtt
    10801 cacgaccatg atgaaaatgg actcctggac gcaaaaagaa acgccagtga agcggtaaaa
    10861 tttgctgcca gtggtgggcg atgtccgctt ccagttcccc attttcctca tttatgcgcg
    10921 ttagcgaact gaagatttgc cgggcgatat gggtttcgga acgacgcaat gcactgccgg
    10981 gcagcagatt acgcaacgga cgatagtaga gaacgcgcag gatgtgccgc ccctggcgga
    11041 agctgcgtcc cagatgagaa accaacattt gccgcacagc cgctttgtcg ccaaccagtt
    11101 gcaccagttg atcgatacga tcctgctcca gcaggtcctc tgctcgctgt tgctgaagcg
    11161 ccagcccggt atagaggaat gtcagacgcg agcgtttgcc gcgcccgact tccgcttccc
    11221 acgtcagcca gccgcgatcc tgcatggtgt tgagcagggt gcgcatatga cgacgcgagc
    11281 agctcaataa cgctgccagt tcgttgagcg tcgtgtcctg cgatttaccc tcgcagcatt
    11341 gccacaggcg gatgaactgt tgttgcagac gagcagatgg cataaaaggg gaactcctgc
    11401 gcaaaagaca gcaattttat tttccctata ttaagtcaat aattcctaac gatgaagcaa
    11461 ggaggtgcct catgcgtcag ttttatcagc actattttac cgcgacagcg aagttgtgct
    11521 ggttgcgttg gttaagcgtc ccacaacgat taaccatgct tgaagaactg atgcaatggg
    11581 aggggagtca ttctgactac tgatttgcgg acatcatgtg tgactgagta ttggtgttaa
    11641 tcgccacgcc agcagtgatt atctgctggc ttttttcttt ttggcgattg agtgccggac
    11701 ggtgtacaaa ctaacgcata aacgccggtt gcttctcttc ataagacgca attgcgtcgt
    11761 cgtgctgcag agtcaggccg atgctgtcca gaccgttcat catgcagtgg cggcggaagg
    11821 catcgatggt aaagcgataa gttttctctc ccgctttcac ctcttgcgct tccaggtcta
    11881 catcaaaatg aatccccgga ttggcctgca ccagcgcaaa cagttcgtcc acttctgcgt
    11941 cgcttaattt caccggcagc agctggttgt taaagctgtt gccgtagaag atgtcagcaa
    12001 aactcggtgc aatcaccact ttaaaaccgt aatcggtcaa tgcccacggc gcgtgctcac
    12061 gcgaggaacc gcagccgaag ttttctcgtg ccagcaaaat ggaagcgccc tgatactgcg
    12121 ggaagttcag cacgaagtcc gggtttggct gttggccttt ttcatccaga aaacgccagt
    12181 cgttaaacag atgcgcgcca aaacccgtac gggtcacttt ctgcaaaaac tgtttcggga
    12241 tgattgcatc ggtatcgaca ttggcggcat ccagcggaac caccaggcct gtgtgtttga
    12301 taaatttctc tgccatggtg tgctccttat ttaatgttgc gaatgtcggc gaaatgtccg
    12361 gtcacagcag cagcggcagc cattgccggg ctgaccagat gggtgcgccc tccacgcccc
    12421 tggcggcctt caaagttacg gttgctggtg gaggcacaac gctcacccgg attcaggcga
    12481 tcgttgttca tcgccagaca cattgagcaa cctggcaagc gccattcaaa accggcttca
    12541 ataaagattt tgtccagacc ttccgcttcc gcctgggctt ttaccgggcc agagccggga
    12601 accaccagtg cctgcacgcc tggcgcgact ttccgtcctt tggcgatttc agctgccgcg
    12661 cgtaaatctt caatacgtga gttagtgcag gaaccgataa acactttgtc gatagccact
    12721 tctgtcagcg gaatacccgg tttcagcccc atataggcca gcgctttttc tgccgacgcg
    12781 cgttcaaccg gatcggcaaa cgaagccgga tcgggaatgt tgtcgttcac ggaaatcacc
    12841 tgacctggat tggtgcccca ggtgacctgc ggtgaaattt cttctgcctg caaagtcaca
    12901 acggtatcga aagttgcgcc ttcgtcggtt tgcagagttt tccagtaggc aacggcgtcg
    12961 tcgaaatctt tgcctttcgg cgcatgcaga cggcctttga catagttaaa ggtagtttcg
    13021 tccggtgcaa ccagaccggc tttcgcgccc atttcgattg ccatattgca cagggtcata
    13081 cgaccttcca tgcttaaatc acggattgct tcgccgcaaa actccaccac atgcccggtg
    13141 ccgcctgcgc taccagtttt accgataatc gccagcacga tatctttcgc tgtaatgccc
    13201 ggcgcggctt tgccctggac ttcaattttc atggtcttcg cacgaccctg tttcagggtt
    13261 tgcgttgcca gtacgtgttc aacttcggaa gtgccgatac caaaggctag tgcgccaaac
    13321 gcgccgtggg tggcggtatg cgagtcgccg cagacaatgg tcatccccgg caatgtgacg
    13381 ccctgttccg gccccattac gtggacgatc ccctgatacg ggtgattcag gtcatacagc
    13441 tcgacgccaa attctttgca gtttttgatc agctcctgca tctggatgcg cgccatttca
    13501 ccgcaggcat taatgtcttt ggtctgagta gagacgttgt gatccatagt ggcgaaggtt
    13561 ttgcctggct gacgtaccgg gcgaccgtgg gcgcgcagac catcgaacgc ctgcggtgag
    13621 gtcacttcat gcaccaggtg gcggtcgata tataacagcg gggtttcgtt ttcggcttcg
    13681 tacactacgt gagcgtcgaa caatttttcg tataacgttt tagccatgat tacacccctt
    13741 ctaccacata gcgggcaatg atatcgccca tttcattggt actaacggcg gcagcgccac
    13801 gggctaaatc cccggtgcga atgccttctt ctaatgcgcg gttaatggcg cgttcaatgg
    13861 cgcaagccgc atcatctgca tccaggctgt agcgcagcag cagtgccagc gacagaattt
    13921 gcgcaatcgg gttggcgatg tttttgcctg cgatatctgg tgctgagccg cctgccggtt
    13981 catacaaacc aaaaccttgc tcgttcaggc tggcggaagg caacatcccc atcgagccag
    14041 tgatcattgc gcactcgtca gacagaatgt cgccaaacag gttggaacac agcaggacgt
    14101 caaactgtga tggatcttta atcagctgca tggtggcgtt gtcgatgtac atatgcgcca
    14161 gttcgacatc cgggtattcc gtggcgatct cgttgacgat ttcacgccat aaaatagagg
    14221 attgcagcac gttggcttta tcgatcgacg tcactttatg gcgacgttta cgagcagatt
    14281 caaacgcgat gcgggcaata cgctcgatct caaaacggtg atacacctcg gtatcaaacg
    14341 ctttttcata ttgtccgcta ccttcgcggc cttttggctg accgaaatag atgccgccgg
    14401 tcagttcacg cacacacagg atgtcgaagc cgttagcggc aatgtcagca cgcagtggac
    14461 agaatgcttc cagcccctga tacagttttg ccggacgcag gttgctgaat aatttgaagt
    14521 gcttacgcag aggcagcagc gcgccgcgtt ctggttgctg gtctggtggt aaatgttccc
    14581 acttcggacc gcctaccgag ccaaacagca cggcatcggc ttgctcacaa ccttcaaccg
    14641 tcgcaggcgg cagtggttgc ccgtggttat caatggctgc gccgcctaca tcgtaatggc
    14701 tggtggtgat acgcatcgca aatcggttgc gcacggcatc cagcactttc agcgcctgag
    14761 tcatcacttc cggaccaata ccgtcccccg gcaatacggc aatatggtaa ttcttcgaca
    14821 tcacacggtt tccttgttgt tttcgttgtg ttgagctttg cgttgcaact ctttttcgac
    14881 ttctgcggca cgccagatat tgttcagtac gtgcaccatg gctttggcgg aggactcgac
    14941 aatatcggtg gccaggccga cgccgtggaa gcggcgaccg ttgtagttgg cgacaatatc
    15001 cacctgaccc agcgcatctt taccgtgacc tttggcggtc aggctgtatt tcaccagttc
    15061 gacgttatag tcagtgatgc ggtttatcgc ctggtagacg gcatcgaccg gaccgttacc
    15121 gttggcggct tctgctttga cttcttcgcc gcaggccagt ttgacggcgg cagtggcaat
    15181 atcgttagaa cctgactgca cgctgaagta atccagacgg aaatgctccg gctcttcttg
    15241 ctgcttaccg atgaaggcca gcgcctccag atcgtaatca aacacctgac cttttttgtc
    15301 cgccagcttc aggaaggcat cgtacaaatt gtctaaatta tattcacttt ctttataccc
    15361 catctcatcc atgcggtgtt ttactgccgc acgccccgaa cgagaggtca gattcagctg
    15421 gatttggttc aaaccaatag attctggtgt catgatttcg tagttttcgc ggtttttcag
    15481 cacgccatcc tggtggatac cggaggagtg tgcgaatgcg ccgctgccaa caatggcttt
    15541 gtttgccggg atcggcatat tacaaatctg gctaactaac tggctggtgc gccatatctc
    15601 ctggtgatta atagcggtgt ggacgttgag gatatcctta cgaactttga tcgccatgat
    15661 gacttcttcc agcgaacagt taccggcacg ctcgccgatc ccgttcattg caccttccac
    15721 ctggcgtgca ccggcatgta ccgccgccag tgagttgccg accgccaggc ccagatcgtc
    15781 gtgggtatgt acggaaataa tggctttgtc gatgttgggt acgcgttcat acagcccgct
    15841 gatgattccg gcgaactcaa atggcatggt gtagcccacg gtgtccggaa tgttgatggt
    15901 ggtggcaccg gcattaatcg ccgcttcgac cactcgcgcc agatcggcaa ttggggtgcg
    15961 tccggcatct tcgcaagaaa attcaacatc atcggtgtaa ttacgggcgc gtttcaccat
    16021 atagatagcg cgttcgatca cttcgtccag cgtgctgcgc agcttggtgg cgatgtgcat
    16081 tggcgaagtg gcaataaagg tatgaatacg gaaggcttcg gcgactttca gggattcggc
    16141 tgccacgtca atatcttttt ccacgcagcg agctaacgcg catacacggc tatttttaac
    16201 ctggcgagcg atggtctgca ctgattcaaa atcacccggc gaagagacgg ggaaaccgac
    16261 ttccatcacg tcaacaccca tacgctcaag ggccagcgca atttgcagtt tttctttcac
    16321 actcaagctt gcctgtaacg cctgttcacc gtcgcgcaat gtggtatcga aaataatgac
    16381 ttgctggctc atggtttggg tccttgtctc tttttgagcg cctcgcttcg ggcataaaaa
    16441 aacccgcgca atggcgcggg ttttttgttt gactgagtgc tgacttaatt ctgaatgccg
    16501 ctcaccagtc taccgcgcaa agaagatgcg tttagtagta gtagaccgat aaagcgaacg
    16561 atgtgagtca ttaaatcagc tccagatgaa tgcgatatgc ttttagagtt actggatacc
    16621 aaaacggatg tcaaccctga tgcaacaaaa acgtccagcc agcgtgagtt ctgcatccgt
    16681 caaattagct aattgtgctg cggtggttaa aacaagcgat attaatttct gcttaaccac
    16741 cgccgctttt catcggttga catatttcag aataaatttt tgcatctaat cgacgaggga
    16801 aagggggata aaatgcacgc gttacaaaac ctatcctgat gatttgtatt gaattatatg
    16861 tattgcgaat tttttgatat tcatttggtg aatattattg a
//
LOCUS       DAZGHF010000103        16722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGHF010000103 DAZGHF010000000
VERSION     DAZGHF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.103"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(89..349)
                     /gene="yfhL"
                     /locus_tag="ACHK3A_003527"
     CDS             complement(89..349)
                     /gene="yfhL"
                     /locus_tag="ACHK3A_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO8741565.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            564..1961
                     /locus_tag="ACHK3A_003528"
     CDS             564..1961
                     /locus_tag="ACHK3A_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8741566.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADCGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            complement(2155..3549)
                     /locus_tag="ACHK3A_003529"
     CDS             complement(2155..3549)
                     /locus_tag="ACHK3A_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFO8741567.1"
                     /translation="MGQIFTPRPYQDLIINHQIETLRGNIWAGMGMGKTVATLTALED
                     LFMAGAETRPALVLAPLRVAASTWPDEALKWGHLRNIEVQPIVGNAKARSAALANSNA
                     SVFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVQ
                     RWINLTGTPAPNGLVDLWGQLWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPF
                     AHSQAEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELN
                     GEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVA
                     YHWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNIL
                     VFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTV
                     QDILLDAMKKRGIA"
     gene            complement(3812..4327)
                     /locus_tag="ACHK3A_003530"
     CDS             complement(3812..4327)
                     /locus_tag="ACHK3A_003530"
                     /inference="COORDINATES: protein motif:HMM:NF021206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO8741568.1"
                     /translation="MFETTLTLAGTGKQPMMSSREIAELTSKEHKIVVRDIKALLEQL
                     GFHSTEMYHHENKDFLVKRKIYNGREVIDEILLDQDLSTTLVTGYSAHDRYKVVKRWR
                     ELETGKVQPLVTHPSTPKIQINDDIVRLARVVAEATMKACLDATGYSQPAAPANLSHV
                     EGEYVPVSKAA"
     gene            complement(4388..4564)
                     /locus_tag="ACHK3A_003531"
     CDS             complement(4388..4564)
                     /locus_tag="ACHK3A_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741569.1"
                     /translation="MAIQITQLHQADIFGYIADMLETARLLSSLEKGEQLAFELIYFA
                     QQAAREAANKPWDE"
     gene            4677..5006
                     /locus_tag="ACHK3A_003532"
     CDS             4677..5006
                     /locus_tag="ACHK3A_003532"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO8741570.1"
                     /translation="MSKRDDPQLRVRIPELLKEALEKKARANKRTLTSEIVSRLESTI
                     LQDKLLHTSRGFEEAADEILILRDLLEKLKTTYHREYQVEWVYENKYELIEVMEKLKK
                     LLNPENE"
     gene            complement(4975..5151)
                     /locus_tag="ACHK3A_003533"
     CDS             complement(4975..5151)
                     /locus_tag="ACHK3A_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO8741571.1"
                     /translation="MSKRDDPQLRVRIPQDLKDTLEKISRENDRTLTAEITRRLRKSL
                     EDESTIHSQDLATS"
     gene            complement(5148..5417)
                     /locus_tag="ACHK3A_003534"
     CDS             complement(5148..5417)
                     /locus_tag="ACHK3A_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO8741572.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFVSPGRRSVPDRIVLLPG
                     GRIVFVECKAPGKPPRPDQLREHERLRKLGFTVVVLDSKNLEGIL"
     gene            complement(5417..5899)
                     /locus_tag="ACHK3A_003535"
     CDS             complement(5417..5899)
                     /locus_tag="ACHK3A_003535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8741573.1"
                     /translation="MTCEQTILDMCCGSRMFWFDKEDKRAVFSDIRAEQHELCDGRQL
                     VISPDLIADFRALPFADNTFPVVVFDPPHLERVGDNAWMRKKYGRLNKETWRDDLRDG
                     FAEAFRVLRPHGVLIFKWNETQIPVSNILALTDEKPAIWQRTGKADKTHWVIFVKGVE
                     "
     gene            complement(5889..6110)
                     /locus_tag="ACHK3A_003536"
     CDS             complement(5889..6110)
                     /locus_tag="ACHK3A_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO8741574.1"
                     /translation="MSFKYRDSPLYYRAAREALRLEQSGEYDRAAKVWAKANRQSRNE
                     LNQDWSERRSDFCLMQNMREKRKAVSDDL"
     gene            complement(6116..6319)
                     /locus_tag="ACHK3A_003537"
     CDS             complement(6116..6319)
                     /locus_tag="ACHK3A_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741575.1"
                     /translation="MTVIKTHTGTVITRTGEKQVQLHQNATTWVAGPKEYYYKDTGRR
                     GGAVGVRTRLVLSSIRPIEAKQE"
     gene            complement(6325..8388)
                     /locus_tag="ACHK3A_003538"
     CDS             complement(6325..8388)
                     /locus_tag="ACHK3A_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFO8741576.1"
                     /translation="MSNILWGDLETYCDIPINNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIAAPETLLYFHNSHFDRTVLRYAMPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDMQLARAAIEAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPDLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDITPDDVDKHMRQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKSWYNESVKRKSTFGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEKGNITYMGVNSYSRKWQRLKTYGGKLVENVTQAAARDVLAGN
                     MPLIEDAGYSIVLTVHDEVITEAPDTEDFNDKALSALLSTNPEWAPDIPLNAGGFEAY
                     HYRKD"
     gene            8450..9139
                     /locus_tag="ACHK3A_003539"
     CDS             8450..9139
                     /locus_tag="ACHK3A_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741577.1"
                     /translation="MTVGNKRQPNGLPEYYCHRGNDIKDAIVRDFIIQPVAHLKLITG
                     QTKTSYSGYPLTDQYYCFSFKKRSDENCTGAFFCGRDVAKDFIRRIGCTELPLFNPMK
                     SDTSAAIERSGSGSGSSGITVKPNADKKLFIDAARLLMTLWDSTSKGQTPLASVLNEA
                     ISGGIDKPPEIRHVVAVNTMLGNTKNTLTGLLDKARVKYPDVLFRDFDFTPLNEIVKR
                     SKSYNKHPPCY"
     gene            complement(9133..9774)
                     /locus_tag="ACHK3A_003540"
     CDS             complement(9133..9774)
                     /locus_tag="ACHK3A_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HFO8741578.1"
                     /translation="MSQPVLTNHLKTQIINNALEKAGIPKRKSALRAARVEWAERVRL
                     AAIGGVEKEAEILKNIKKIESMVTKIPDSLKTNSSLIRKDCCIYLNLAGARANIYFNG
                     NYRGYESGAPEHINKIAPAEFTLLGEDPLVTEFYSFDALYTQIKSDETEIIQNVSAAL
                     NKVRTVKQLLDVWPEAKELLPTDAPPVPLAPAVHRETLNEMIGLPTNTVSDTQ"
     gene            complement(9826..10374)
                     /locus_tag="ACHK3A_003541"
     CDS             complement(9826..10374)
                     /locus_tag="ACHK3A_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFO8741579.1"
                     /translation="MKIKLNNVRLAFPALFEAKTVNGEGDPRFSAVFLMSPKHPQLEE
                     IRKAMKQVAKEKWGEKWESIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            complement(10390..11691)
                     /locus_tag="ACHK3A_003542"
     CDS             complement(10390..11691)
                     /locus_tag="ACHK3A_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFO8741580.1"
                     /translation="MPDVHAQLSPSSAHRWLRCAGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVEAVGRYVDTV
                     WTLAKNNELLVEQRVDFSHITGVEESFGTADGIIIAGSELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGERALEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGHPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDLKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLAADLI"
     gene            complement(11694..12596)
                     /locus_tag="ACHK3A_003543"
     CDS             complement(11694..12596)
                     /locus_tag="ACHK3A_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741581.1"
                     /translation="MSLETSLELNNQLLAQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATEAENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKRASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            complement(12593..12742)
                     /locus_tag="ACHK3A_003544"
     CDS             complement(12593..12742)
                     /locus_tag="ACHK3A_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741582.1"
                     /translation="MKPEHLYRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            complement(13470..14099)
                     /locus_tag="ACHK3A_003545"
     CDS             complement(13470..14099)
                     /locus_tag="ACHK3A_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO8741583.1"
                     /translation="MNLANRAKKRRTELNLTQVEVAKRAGISQQSIEAIENGKTLKPR
                     NLLALASALECDPKWLLLGGDVVTDFNYGARRVPVLSYVQAGAFTSAELLREEGDFEY
                     ILTTAELSESSFALRIRGDSMEPEFKEGDIVIIDTDVYPTPGEFVAACNGSHEATFKK
                     YRPVGIGAKGEEDFELVPLNSDYPICRSWEKPIKIIGTMVEHRIFRRKR"
     gene            14207..14431
                     /locus_tag="ACHK3A_003546"
     CDS             14207..14431
                     /locus_tag="ACHK3A_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8741584.1"
                     /translation="MTSSTLASRIKERRKALGITQTSLAESVGMRQQSVQYLESGRAT
                     RTGFILELAKVLKCDPDWLLNGENSEQQKV"
     gene            14435..16654
                     /locus_tag="ACHK3A_003547"
     CDS             14435..16654
                     /locus_tag="ACHK3A_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFO8741585.1"
                     /translation="MPDNKPWGATPDEWFHFDLVLGMTDRLLPVVSNPCAELSPNSKL
                     SHNFGKTPSRYNGQRKVVGIPDWANYVVTENDFSRWSNEPDYGICVRTGDGVVALDCD
                     INDAGMQEIVRNIILSCLGELPPRRWRADSHKCLYLIAVDGDYRKRGHRLEGENKQIE
                     LLAKGQQFVACGTHPAGERIQWDCGLPGEPLKITSEQLESLWQRLADNLPVKDSYTAG
                     AGRQRDLSCVDPSATDDVADWLDANGWTLSVSSDGSRNLKPFRDESEYSDGCSETSIK
                     YFPKGTGGFEQGHFKSMHNTDAGLTDADWLDAYGYRVADFEDLTVAEDGDKPEYLPWP
                     KFSRDKNDRIEGTVTNVLLALRRPDLCGVQIRLDEFRDEIILTTPRGKNLRLRDEFYT
                     MLHSRLECLGFKKFEEAAIRRAVRLVAYENRFDSLKDWIANLPAWDGVPRIDTFFCRH
                     WGIEANAYTHAVGRYWWTLLAGRALVPGIKGDMAVILVSQQGKNKSEGIRAMAPTPEH
                     YMELDFGKKGEERIREMRGHNVIELGEMRGMDKRGIGDVRVTISTRADRNRGLFREHY
                     VTLPRRCGFIGTSNSDTPLTDTEGNRRWLPMTIPDETDGKFIAQGIADEKIQLWAEAV
                     AVFKRDGIAWENAETLAKSILGDYEVKDEVWMSCVAEWLELPQDLYDMAADGTKKNCE
                     RVPLTSKDVLVEAIGFKAPQVKRSDEMKIAAIMKKLGYKNKQLQSLPGKPRSWEKCK"
BASE COUNT         3940 a         4047 c         4193 g         4542 t
ORIGIN      
        1 aattatgcta atgtcatcaa tcaaataaat ataatagcgt taaggtttct ctataaaata
       61 attaacactc tacatccagg gtagaatatt aaattttatc cgcgtgatgc ataagcacaa
      121 atttatccca caactgttct tccgtctcga catgttgcgg atccttcaca atagtattgg
      181 ggatcgggca caccttctgg caggttggtg tctcgtagtg ccctacgcat tcggtacact
      241 tatcgctgtt aatctcgtag atatgatcgc ccattgaaat cgcctcattc gggcattcgg
      301 gttcacacat atcacaatta atacagcgtt tagtaattag taaagacatt tcaatgaatt
      361 accgttaaat cattttaaaa tcagtaagtt gtatcgagtt tgtatgcttt actgtcatta
      421 acttactgta tgttgatcca gtgtatttaa ccttgataaa ctcagtccag caacacaaaa
      481 ccgcaacaca ttgcattttg tcccgtaaaa aagacttgta tgtgtgtgct tgttttctgc
      541 gcctacgcag ataaggattg agaatgccgc gcactgtaac acataatccg gatagcccca
      601 ataatgacga tgttttagcc gcatctgaaa aatgggacgc ctgtaaaccc ccctatacca
      661 gcgcacacat gaaaatctgt gttgctgccg ccaaaatcat cctcgctgct tccggcgtgg
      721 ctcgccgttc caaatacgaa aaagagaact atctccgtat cgatttcagc aaagccggta
      781 aggttacatt ttacgccgag tttccaaaaa agatgggcct caagggtaaa aagctcggcg
      841 agtggccgga gctcgctatc cagctggcgc gcgaaaaagc gctaggtatg gctgactgtg
      901 gcttgcgggc agagtcagta catgcagcgc tggaaatgta ccgggatgac ctcaaagcca
      961 aagtcgcccg gcagaagctg agcccggaca gtttcacaac ctacggggtg cgtatcgacc
     1021 ggattaaagc aacgttcggc gagcgtgagg tgttcagcga cgtaacatac aatcggctgg
     1081 tggaagtgct ggacgagtgg atcgccactc gctcgaacaa taacgccctg gagttgtttg
     1141 ccgagctccg tcggttctgg aagttctgcg cacctactct ttgcaacggc cgcaatgttg
     1201 ccgccagtct gccagatgat tatgtttcct cccgcgtaca gaaacctacc cccacacggc
     1261 tttttaccga tattgaatca atcgcccgac tctggctcaa tgttgctgcc tgcacctctg
     1321 tacaccagaa gaatgctgtt cgcttcatga tcatcactgg cgttcgtccg attaatgtcc
     1381 ataacctgcg ctgggactac gttcacgagg aggctggtga aattgtttat ccggaagggg
     1441 ttatcggcat gcgaggggct atgaaaacac aaaaggcttt ccgcctgccg ataacgcctg
     1501 agatccggcg gattatcgac gagcagaaag cctggcgtga ttcagttcct gagtgcaaca
     1561 gggattatgt atttttgcag ccacgtgatc caatgcagcc attttcaaaa cgatcactgg
     1621 ataagctggt gaaaacatac agcccggacg gggctgtaaa aggaataaaa catgatggga
     1681 ctgttaaagg gaaagacggt gcatttaata cgatgtgccg taaattcctt aagagcaatg
     1741 ttattgcctt gatgaaggaa agaggctatt cccgatcaga ccgaagggaa atcagcctcc
     1801 tttgccttca ccactccagc aagtcagatg acccgatggc agaacattac gacttttctg
     1861 atgagatttt gcaggaagag attgcgttaa agcgcgaagc tttcgaggct cacgagcgga
     1921 gcatacttgc gcaggtggca ttgctacggc ggcgaggtta atactggctg cgacactttt
     1981 gaataaaagc gtcgacattt cggcgctcat aacgaactac ttttgcactg aaacgaattg
     2041 gtgccaggat agcccgatga cgatgcttaa tattccactc acatagcgtt ttctgtgtaa
     2101 tacctaactt ttggcatact tcatccgggg tgagtaaatc gtcgggtttc tcgctcatgc
     2161 tatacctctc tttttcatgg catcgagcag gatgtcctgc actgttcgtt ttgagttgcg
     2221 ccgctccatc accatttcgt ccatagtgtc ggccgcaata atgtggtgaa taaataccgg
     2281 acgattgtgt ccggcctgta tctgcctggt ggggccgata cgttcaataa tttgctgata
     2341 ctgctccagg tcccaccagt gtgagaaaaa taccagtatg tttccgccgt cctgcatgtt
     2401 cagaccgtgg cccgcgctgg ctgggtgtgc aaagagaaca ggaatctttc cggaattcca
     2461 gtcgcgcagt gtctgtggat cttggtcgag gtgacgaccg cgagggaatg ctttaagcaa
     2521 gcgttcaaga tcgtgtttcc agtgataagc aaccagcaca ggtgcgccag ctgcttcggt
     2581 cagtatgctg tccagcgcct gtagtttggt gtcatgcagt tctgaccaac ttccggtgtc
     2641 gtctgtgtat actgcgccac tggcgatttg aagacacttc agtgtctttg ccgcggcgtt
     2701 cggtgcttcg atgccttcgc cattcagctc gaggaacatt tccttttcca tttcacgata
     2761 ctgctgacgg gccttcggcg gcatatccac gcggattacg ttatggatgg ggtctttgat
     2821 atcgaaccag tcggccgcat ccagcgagag ggtcacatcg gctaacgctc gctgtatttc
     2881 agcctgtgag tgagcaaacg gctccagttt ggtccagctc tgccccggaa actgtatcga
     2941 gttgaaccag cgtgaggtaa acgcgccgta agtgcgcccg agacgttgcc cctggtccac
     3001 aaaccacaat tgtccccaca aatctaccag gccgttcggt gctggcgtac cggtgagatt
     3061 tatccagcgc tggacatact tatgcgccac ttttcccagc gccgccgcgc gcttaccacc
     3121 acctcgcagc cggaaggatt ttagccgggt gctttcatct ggaatgacag taccgaacgg
     3181 ccatcgttct cccaattcct caaccagcca gacaaggtta tcgtagttga tggtgaacac
     3241 gctcgcgttg ctgttcgcca gcgctgcaga gcgcgctttg gcgttaccaa caatcggctg
     3301 cacctcgata ttgcgcagat gcccccattt cagcgcttca tccggccatg tgcttgctgc
     3361 aacgcgtagc ggcgcgagga ccagcgcggg gcgtgtttct gcccctgcca taaagaggtc
     3421 ttccagcgca gtgagcgttg ccacggtttt acccattccc atccccgccc agatgttgcc
     3481 gcgcagggtt tcgatttggt gattgatgat caggtcttga tacgggcggg gggtgaaaat
     3541 ttgtcccata gtgttttagc cagtagggcg cgggtaatcc cgcgcaataa tgtcagatta
     3601 gatcggtgta gtaggtgaag ttgccaaact gcggatgtct ccagcgcttg cggttacctt
     3661 tcggcggtgt cgattcgttt aacagctttt gagctgcttg ttcgatcgct gggaggttaa
     3721 cgagcaatcc ccgaacaccg gtgtctgaac gtaccggtat cctggcgaag ccgatcaatt
     3781 tccggcatgt tgaatcagat aaacctgttt tctatgctgc tttgctgacg gggacatatt
     3841 caccttcaac atgagataaa tttgctggtg cggctggttg agaatagcca gttgcatcaa
     3901 gacaggcttt cattgttgct tctgctacga cgcgagcaag ccggacgatg tcgtcgttga
     3961 tctgaatttt gggtgtggaa ggatgggtaa caagcggttg tactttacct gtttccagct
     4021 cacgccagcg cttaactact ttataccggt cgtgggcgct ataaccagtt acaagcgttg
     4081 tggaaaggtc ctgatcgagt agtatttcat caatgacttc gcgaccatta tagatcttcc
     4141 ttttgactaa aaagtcttta ttttcatgat ggtacatttc tgtactatga aaacccaact
     4201 gctcaagaag tgctttaata tcgcgcacga ctattttatg ttctttgctg gtaagttctg
     4261 caatctcgcg actgctcatc atcggttgct taccagtgcc tgctagtgtt aaagttgttt
     4321 cgaacattga agtattctcc attcagtaaa agatgcgctg gtagctgcaa ctatcagcgc
     4381 gttttgttta ttcatcccac ggcttgtttg cagcttccct tgcggcctgc tgcgcaaaat
     4441 aaatcaattc aaaagctaac tgctctcctt tttccagcga actgagaagt ctcgctgttt
     4501 ccagcatgtc cgcgatatac ccgaaaatat ctgcctgatg taactgcgtg atctgaatag
     4561 ccatagcgtt aaccttgttt tgtactcatt gtgaggactt tattagtact cacaatgagg
     4621 cttgaagtca acatgtttgt gaggcatgtt gttctcacac tttcaaggga tcatatatga
     4681 gcaaaagaga cgacccgcag ttgagggtac gcataccaga attgctaaaa gaggctttgg
     4741 agaagaaagc acgggctaat aagagaacac tgacctctga gatcgttagc cggttggaat
     4801 ctactattct tcaggacaaa ttacttcata catctagggg gtttgaggaa gctgctgatg
     4861 agattctcat actcagggat cttctcgaga aacttaagac aacgtaccat cgagagtatc
     4921 aggtcgaatg ggtttatgaa aacaaatacg agcttattga agtgatggaa aaacttaaga
     4981 agttgctaaa tcctgagaat gaatagtgga ttcatcctct aaacttttac gtaaccgccg
     5041 ggtgatttcg gcggttagtg ttctgtcgtt ttctctggaa attttttcca gtgtgtcttt
     5101 caagtcttgc gggatgcgta cccgcaattg tgggtcatca cgtttactca caatattccc
     5161 tccagatttt tgctatccag caccaccacg gtaaagccca gctttcgcag acgttcatgc
     5221 tcgcgcaact ggtcaggccg tggtggcttg cctggcgctt tgcattcgac aaagacgatg
     5281 cgaccgccgg gtagcaggac aatgcgatcc ggtaccgagc ggcgaccggg tgacacgaac
     5341 ttaaaggcga ccccgccagc ctttttcact tcggcgacga ggtgcttttc gataaggctt
     5401 tcacgttcgt aggccatcat tccacacctt tgacgaaaat aacccagtgg gttttgtcgg
     5461 cttttccggt tcgttgccat atggccggct tctcgtcggt cagcgccaag atattgctta
     5521 ccgggatctg cgtttcgttc catttgaata tgagtacacc gtggggccgc aataccctga
     5581 atgcttcggc gaagccgtcg cgaagatcat cgcgccatgt ttctttgttg agccgcccgt
     5641 actttttcct catccatgcg ttatcgccga cgcgctcgag gtgcggcgga tcgaaaacga
     5701 cgacagggaa agtgttatcg gcaaagggaa gggcgcggaa atcagctata aggtccggac
     5761 ttataaccaa ctggcgaccg tcgcaaagct catgctgctc ggcgcggata tcactgaaca
     5821 cggcgcgctt gtcctccttg tcgaaccaaa acatacgaga gccgcagcac atgtcaagaa
     5881 tggtctgctc acaagtcatc gctcaccgcc ttacgctttt cgcgcatgtt ctgcatcaga
     5941 caaaaatcag accggcgttc gctccagtcc tgattcagtt cgttacgtga ctggcggtta
     6001 gccttggccc agaccttcgc tgcccggtca tattcgccgg actgctcaag gcgcaaggcc
     6061 tccctcgcag cccggtaata aagcggactg tcccgatatt taaatgacat aggggttact
     6121 cctgctttgc ttcgattggg cggatgctgc tcagcaccag acgagtgcgg acaccgacag
     6181 caccaccgcg gcggccagtg tctttgtagt aatactcttt tgggccagcc acccacgttg
     6241 tcgcgttttg atgcaattgc acctgctttt caccggtgcg ggtaataacc gttccggtat
     6301 gcgttttgat tacagtcata gcgattaatc cttacggtag tggtacgcct caaaaccgcc
     6361 agcgttcagt gggatatcgg gcgcccattc ggggttagtg gagagaagcg cggaaagcgc
     6421 tttatcgttg aaatcttctg tgtcaggtgc ttcggtgatc acctcgtcgt gtaccgtcag
     6481 cacaatgctg taaccggcat cttcgataag cggcatgttt ccggccagaa cgtcgcgggc
     6541 ggccgcctgg gtgacgttct ctaccagctt tccgccgtag gttttgagtc gttgccattt
     6601 acgcgaataa gagttaacac ccatataggt gatattccct ttttcgataa ccggagaggg
     6661 gtagcataca gcgcgtccgg atggtagctg tatgcgcagc cacgcgccat cgcggcggat
     6721 tttaagataa ccgcaataca atgttttttg cggtgtggcg attgcagtgc ggacggtgcg
     6781 ctccagttcg taccagaaat cgcaggtcgc cgggtgcgcc ctgcgccaca ggcgcttgag
     6841 cgagtcacat gcgatgaata cacgctcgga caggccaaag gtcgacttac gtttaaccga
     6901 ttcgttgtac cagcttttcg cctcgcggat aacatcgcgg ggaatgtttg gcagtgcggc
     6961 gttcgccagc tcgtcgagat cgagaccgta aaccagagca aaagtgatga aagccgatac
     7021 accacctcca taacccagac cgagttccat gactttaccg atctgacgca tgtgtttatc
     7081 aacatcatct ggtgtaatat cgaaagcttt tgcatacgcc agtttatata agtccggacc
     7141 cgttccggcg tcgtactctc tgaatgcatt cagtttccat tcttctcccg ccagccatgc
     7201 cagcatacgg ccttcaatgt ttgacaagtc acttaccacc agttttttgc ctgttggcgc
     7261 gataatgcag ccacgtaacg ctgaactggt tagttccatg atattgtcaa acagcaggtc
     7321 tgcacatccg gctttcagtg cttcgatgcc ttcgtctatt tgttcctgtt ttagtgaagg
     7381 acggggaagg ttctggggct ggaatagccg tccggcccaa cgaccggtac gtgacgctcc
     7441 gcagaactgt agcgtaccgc gtaagcgccc gtcgtggctt acgcctttca tcagtgcctt
     7501 gtatttactg gtgctggtag tactggcctg caggcggata gccagcagtt ctttcacggc
     7561 agatggtaaa tcggggtcgg ctatacgacg ttccagggta ctgcgttgca tgtctggtag
     7621 ctccacaccg taggattcaa cgatgtgctt aatcaacgcg tcccgttgtg tggctgcctg
     7681 cacttcgcca tcagtcattt cctgtgtacg ctttgccaag cgcttttgct cttggtctac
     7741 cgcttcgatc gcagcgcgcg cgagttgcat gtccatgcag acgccccggt cgttgatctg
     7801 ctgatcacga tgccagagcg ccagctctgt cccctgataa ttccacttcg gcagacgttt
     7861 atagacttcg cgcattgcct cgatatccag tccggcgtaa gcaacaaagc gccgccattc
     7921 ttccgggtgg gttttactgg tggcccgacg cagtttgctg tttttcgggc gtggcttaca
     7981 gaacagctgg atcagcgctt taccttcttt gtccttcgct ttgtcttgcg ggacgcctag
     8041 tacttcgcag agttccccca gagaccccgg gagaccgtgc gccagcgcct gaaccatcgt
     8101 gtcgcgccaa cgttcgactg gcggtgccag tcgcggcatt gcataacgca gaacggtgcg
     8161 gtcgaagtga gagttatgga aataaagcag ggtttcaggg gctgcgattg cttcgtataa
     8221 gccgtgtgga ataccaccac cggcagtgat atcccacacg tttactggcc cgtcgttgat
     8281 agcccatgcg aaaagcatca cttcaacgcc ttcggcatac gcatgggtac cgttgttgat
     8341 cggaatgtca caataggttt ccaggtcgcc ccatagtata ttggacataa aatatcctca
     8401 tatggtatat ggttaaatgc tagatatcat gaattaaagg agataactaa tgactgtggg
     8461 taataagaga cagcctaatg gattacctga atattattgt caccgtggta acgacataaa
     8521 agatgcgata gttcgggatt ttattattca gcctgttgca cacctgaaac ttattactgg
     8581 acaaactaaa accagttatt ccggttatcc gttaaccgat cagtactatt gtttttcatt
     8641 taaaaaacgc tctgatgaaa attgtacagg ggcttttttc tgtggtagag atgttgcaaa
     8701 ggatttcatt cggcgtatcg gttgtacaga gctgccgtta tttaatccta tgaaatcgga
     8761 taccagtgca gcaatagaac gttccggttc tggctcaggt agttctggta ttactgtcaa
     8821 gccaaatgct gacaaaaagc tatttataga cgcagctcgt ctgttaatga cgctgtggga
     8881 ttcaacaagt aagggacaaa ccccactggc gtcggttcta aatgaagcaa tttctggtgg
     8941 tatagataaa cctccagaaa ttagacatgt agtggcggtt aatacaatgt tgggtaacac
     9001 aaaaaatact ttaactggcc tgctggataa agcaagagtg aagtatcctg atgtgttatt
     9061 cagagatttt gatttcacgc ctcttaacga gattgtaaaa cgttcaaaat catataataa
     9121 gcatccccct tgttattgag tatcagacac tgtattggta ggaaggccta tcatttcatt
     9181 taatgtctct cgatgtactg ctggtgctaa agggactggc ggtgcgtctg ttggaagcag
     9241 ttctttagct tcgggccata catccagtaa ttgttttact gtccgaactt tatttagagc
     9301 cgcgctgacg ttctgtatga tttcggtttc atcactttta atttgtgtgt agagagcatc
     9361 aaagctataa aactccgtta ccagaggatc ttcgccaagt agagtaaatt ctgcaggtgc
     9421 tattttatta atatgctcgg gggctcctga ttcataacct ctgtagttgc cattgaaata
     9481 aatgtttgct ctggctccag ccagattgag atatatgcag caatcttttc gaattaaaga
     9541 actattggtt tttaatgaat cagggatttt tgtgaccatg gattcaatct tttttatatt
     9601 ttttaatatt tcagcttctt tctctacgcc cccgatagct gcaagccgaa cacgttcggc
     9661 ccattcaaca cgagcagctc gtagtgctga tttacgtttg gggatacccg ctttctcaag
     9721 tgcgttattg atgatttgag ttttaaggtg attagttaat acgggctgtg acattattct
     9781 ttccctcatg agatacccca gcacgtagct gggggatgaa tatttttaaa ttagtgcttc
     9841 agcatcagca ccttcgctga tatcgtcgaa atcgtcagcg cttgccactc cgccgccagc
     9901 gaatgcatcg ccgtctcgca ggaactggac tccgccgagt gaggcattaa tgcgtttacc
     9961 gaaattattg tcctgtgccc agatatcgat aacggcgttt acatagcacc ctgcataggg
    10021 acgtccatca gcctgaatta gtggcgaacg atcgcgatca agaacagctg ggcgcgcttt
    10081 gttagcagcg ttcaggaaga aattgccggg gaagccttca tactctgctt tttcatcacc
    10141 atcatgcagg cacagattga gttttttctc cagctggtta taaatggact cccacttctc
    10201 cccccatttt tccttcgcta cctgcttcat agctttacgg atttcttcca gttgtgggtg
    10261 tttgggagac attaaaaata ctgcggagaa acgcggatcg ccttcgccgt ttacagtttt
    10321 agcttcaaac agagcaggaa aagctaatct cacgttattt aatttaatct tcatgaaagt
    10381 ctcctttaat cagatgaggt ctgcggcgag cgtatcgtcg gatacgtcgt cgaaatcatt
    10441 tacagggttg atattgagtg cggggcgtgg gtccgactcg ggaacgacgg ttggtttacc
    10501 atcagctcgt gttatcagtg cctcgacttt tgaccaacgg cgcggactgg cctttttgat
    10561 aagtttttcg gctttggttg ggctaatcag cttaagatcg aaaacctcct cggttttata
    10621 acggaactgg tctttcagca gcgcgcgggc ggcttcttca tcactccagg cccggttacc
    10681 ttgttttcct gttaccagtt taaaccccgg taccggatgt ccggcattga gttcattgtg
    10741 aacccggtcc cgtactgcct ttagccagga ttcaataaag tcggcctgac tatagatctc
    10801 cgcaagctgc tcaatggtta acagaggtac acgtgcgctg gcattggtga ttatttcgct
    10861 gacaggcttt gtcagatctt caaaatcgct ggccgctgtt tgtaaatgct gcattttctg
    10921 ggcagtgcaa atagcttttg ctttacagaa gcggcactgt ttttctccag gtatgaagtt
    10981 ttccagcggt agtgtctcaa tgccttcgca ttcagcaata ttgagaacaa ggatcgcact
    11041 ggttgcggcc tccagtgccc gttcaccgaa agactgaagt tcctgtacgg ttaaagacca
    11101 ttctgaaacg tggttgagcc ttggctggtg aataaataat cttacagtct caaagtcata
    11161 cagcatgctg aattgttcga gagcacccag agcatacagt tgtagttgct cattttgttc
    11221 tgcatcaatg cggacgcctt tgccatattt caggtcgtgg atttgtaatt cgctaccagc
    11281 aatgattatg ccgtcggcag ttccgaaaga ttcttccaca cccgttatat gtgagaaatc
    11341 aacacgttgt tcaaccaata gttcattatt ctttgcaaga gtccagaccg tatcaacata
    11401 ccggccgacg gcttcgacca tttcatcatc cacctgtggg ccagatgtat catcaggatt
    11461 ttcgcgaagg gggtatgagc cgagaaacat agaaacattg catccggcgt agtgttccgg
    11521 gtggctttgc ctgtttcgta gaactttttc agcaagcgcg tgcgctgcag tgccctcgat
    11581 tgcaaaagtt gtttctttat ccggttgtgt ggcctccagc gccagactac ccgcacacct
    11641 tagccaacga tgtgcagatg atggagaaag ttgtgcatga acgtctggca tgattaaccc
    11701 tccagtgctt tttcagccag ggtgattact tcagcgagat tttcatccgt tacttcacca
    11761 agtttcctgg ctccctgttt ttccagaatt gcaatagctt ctgcccggta accccctttt
    11821 gctaactgga ggatcaaccc ttcagcttgt ttgcgtagtg ccgcgaaatc aattgtatgg
    11881 tcatctttgg cgtcattatt ctggctggaa tttgctgcgt ctctgcgtgc aaattcttcc
    11941 tgcagctgaa ggtactcaac acggttgatc tcgatatggc cttttttcag catctcgttc
    12001 aacttgcgta aggtgtggag ttcactggct gctgtgccgg atacattttt gacgtaaaac
    12061 ggccccgtgc gttctccatc tttgttactg gcccttttcg gcttaacttc atcacgccca
    12121 tctgcaggtg catcaagtag ctgctcggca aaagcacgtc gctcgccgat ggttggcagg
    12181 tcgtcccaga acttaagaat gttacgggac aggtccagga gagcaggttt aagcagcgcc
    12241 ctggctcgtt tgacgccctg taatgcgctg tcgagagcat caatctgaac tactcgttta
    12301 tcgccttcag catcacggta ggcaacagca cgttgcagca tgtcttctgt gataggggtg
    12361 gctaccgggt agaaaccagc cagtgcgata acgtcgctga actccagatc atccagtgtc
    12421 attgccgctg acatgttttc agcttcagtt gctgtatccc gacattcctg cactcgtgaa
    12481 atcgtgtcag gatgcataac aatacctgat gccattgtgc ggataagacg ttcaagcagc
    12541 gcattatgtt gtgccagaag ttgattatta agttcgagac tggtttctaa actcatactg
    12601 tggtcctcgc tacaaggaga atgaaagtga tgatcagacc gagcgcagtg gcaacggcca
    12661 gaccggtcat caaatcgaag tttttacggc gataacggag aacatcgcgc cccgtcagtc
    12721 gatagaggtg ttcaggtttc attggttgta ttcctttttt catatcgggg agcacgctgt
    12781 tgcgagtgcg ctttcagaca taaaaaagcc cgtcacgtga ggcgggcaaa gactacacac
    12841 agcaattaca tggatgattc agttggtatt gtgcgtacga ggtaccacaa ggttgaagcg
    12901 gacacgccag tgcgttgcaa tggcgtacag gaaagcacca cattcagggc agtgctcata
    12961 acattcgttg atttggtatc gttcaggctt tggtgcctga agttgtgctt ggcaagcagg
    13021 gcattcacct ttgagcggtt tatatgtttt cacaccgttt cctcattcag ttctctttgg
    13081 tggttcggct gctgcggtct gagcgtttta cttgtgcatg atgttttcct ccattggttc
    13141 agacgcccca tcatcggggc gtttcaattt gcgtgactta tcagctcgtc gcggtgttgt
    13201 cctctacgct taccgtacgc atacggactc ggcgcttacc tcgatcccat cgggtgccat
    13261 ttcattttgc caggagtact gcggcttacc tgtcacgcgg ttcagtttgt taaagagcga
    13321 ttacttattg cggttaaaaa tacaagcaaa cctgtaagtg cgtcaacagg tattctggta
    13381 ataaaatgca ttattaacct aattacttgt ttttacaggt aataaaaaaa gagggcctaa
    13441 gccctctttg tttagtagtc aggatgcgat tatctttttc tgcggaatat gcgatgttct
    13501 accattgtcc ctatgatctt aattggtttt tcccaggagc gacaaatagg gtaatcacta
    13561 ttcaatggaa caagttcaaa gtcttcctca cccttagcgc caatccctac cgggcggtat
    13621 tttttgaacg ttgcttcgtg gctaccgtta catgccgcaa cgaattcacc aggagtaggg
    13681 tatacgtcag tgtcaattat aacgatgtcc ccctctttaa attcaggttc cattgaatcc
    13741 ccacgtatac gtagcgcaaa tgaactttct gatagctcag ccgtggtgag aatgtattcg
    13801 aaatcacctt cttcacgaag tagctcagcg cttgtgaaag cccctgcttg tacgtagctt
    13861 agaacaggca cccttcgggc tccgtagttg aaatctgtaa caacatcgcc cccaagaagt
    13921 agccatttag gatcgcattc aagagctgac gctaaagcaa gaagattgcg tggtttaagt
    13981 gttttcccgt tctctatagc ttcaatcgac tgctggctaa tgcccgctcg tttcgcaact
    14041 tcgacctgag ttaagtttag ttctgttctg cgttttttag ctcggttggc aagattcatg
    14101 ttgactcctt ttctgtcatt gatgattaca gatacttctg tatttgacaa acaaggttgc
    14161 ctgtgaggta attacaagta aacctgtaat aatgagggct tactcaatga ccagttctac
    14221 ccttgcttca cgtataaagg agcgccgcaa agctttaggt ataacccaaa cttcattggc
    14281 tgaaagtgtt ggcatgcgcc agcagtctgt tcaatacctt gagtcaggac gcgccacccg
    14341 taccggtttc atccttgaat tggcaaaagt tctgaaatgc gatcctgact ggctattaaa
    14401 cggcgaaaat tcagagcagc aaaaggtgta acccatgcct gataacaaac cctggggagc
    14461 tacgcccgat gagtggttcc acttcgatct ggtgttgggg atgacagaca ggcttttacc
    14521 tgtggtgtcc aatccctgtg cagaactgtc gcctaatagc aagctgtctc ataatttcgg
    14581 caaaactccc agtcgctaca atggccaacg taaggttgtt ggtatccccg actgggctaa
    14641 ctatgttgtt acggaaaacg atttttcccg gtggtcgaac gaacccgatt acggtatttg
    14701 cgttcgtact ggcgacgggg tggtggctct ggactgcgat atcaatgacg ccgggatgca
    14761 ggagatcgta cgcaatatta tcctgtcatg cctgggggaa cttccgccac gtcgctggcg
    14821 ggctgacagt cataaatgcc tgtacctgat tgctgttgat ggcgactacc gtaaacgtgg
    14881 tcaccgcctg gaaggcgaaa acaaacaaat agagctgctc gctaagggcc agcagtttgt
    14941 ggcttgtggt acccatccgg caggcgaacg tattcagtgg gactgcggtt tgcccggcga
    15001 acctctgaaa attacatctg aacagctcga aagcctgtgg cagcgtctgg cggataactt
    15061 acccgtgaaa gacagctata ccgcaggtgc aggacgtcag cgtgaccttt cctgtgttga
    15121 tccttccgcg acagatgatg tcgccgactg gcttgacgcg aacgggtgga ctctgtcagt
    15181 cagctctgat ggttcccgca atctgaaacc gtttcgggat gagtcagaat acagcgacgg
    15241 ttgcagcgaa acatccatta agtatttccc aaaagggacg gggggatttg aacagggaca
    15301 ttttaaaagc atgcacaaca ccgatgcggg gcttactgat gccgactggc tggatgcata
    15361 cggctaccgc gtggcggact tcgaagattt gacagtggct gaagatggcg ataagcctga
    15421 gtatcttccc tggcctaagt tcagtcgtga taagaatgac cggatagaag gtaccgttac
    15481 caatgtgctg ctggcgctgc ggcgaccgga tctctgcggt gtacaaatcc gccttgatga
    15541 gttccgggac gagataatac tgaccactcc acgggggaaa aacctgcggt tgcgtgatga
    15601 gttttatacc atgctgcact ctaggctcga atgtctggga ttcaagaaat ttgaagaggc
    15661 agcgatcagg cgtgcggtgc gcctggtcgc gtatgaaaac cgttttgatt cgcttaaaga
    15721 ctggattgcc aatcttcctg catgggatgg tgttccccgt atagatactt tcttctgtcg
    15781 gcactggggt atcgaggcca acgcctacac ccacgcagtc gggcgctact ggtggacttt
    15841 gctcgcgggt cgagctctgg ttcctggcat taaaggggac atggctgtga tccttgtcag
    15901 ccaacagggt aagaacaagt ccgaaggtat ccgtgcaatg gctccaaccc ctgagcatta
    15961 catggaactg gacttcggta agaagggaga ggagcgcatt cgtgagatgc gagggcataa
    16021 cgttattgag cttggtgaga tgcgcggtat ggataaacgg ggtattggtg atgttcgtgt
    16081 caccatatcc acccgtgcag accgcaaccg cgggttgttt cgggagcact atgtcacttt
    16141 accgaggcgt tgcggtttta ttggtacttc taactccgat acgccattga cagataccga
    16201 aggtaaccgc cgttggttgc cgatgacgat accggatgag actgacggca agtttatcgc
    16261 tcagggtatc gctgacgaaa aaatacagct ttgggctgaa gcagtcgccg tttttaaacg
    16321 tgatggcatc gcatgggaga acgctgaaac actggctaag tctattcttg gggattatga
    16381 ggtcaaagat gaggtctgga tgtcatgcgt cgctgagtgg ttagagttgc cacaggatct
    16441 gtacgatatg gctgccgacg gcacgaaaaa gaactgcgaa cgcgtccccc ttacgtcaaa
    16501 agacgtattg gtcgaggcga ttggcttcaa agccccccag gtaaaacgca gtgacgaaat
    16561 gaaaattgca gcaatcatga agaagcttgg ttacaaaaat aaacagttgc aaagtttacc
    16621 gggtaagcct cgcagctggg aaaaatgcaa ataactacaa gtttgtagat ttttgcaggt
    16681 tgttactcaa cctactcaac ctcacaaggg taggttgagt aa
//
LOCUS       DAZGHF010000104        16689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGHF010000104 DAZGHF010000000
VERSION     DAZGHF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.104"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            270..770
                     /gene="tssB"
                     /locus_tag="ACHK3A_003548"
     CDS             270..770
                     /gene="tssB"
                     /locus_tag="ACHK3A_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO8741586.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKVDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            829..2367
                     /gene="tssC"
                     /locus_tag="ACHK3A_003549"
     CDS             829..2367
                     /gene="tssC"
                     /locus_tag="ACHK3A_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO8741587.1"
                     /translation="MSVQQEYATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVKIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDVNLSLVSQMP
                     KAKA"
     gene            2387..3724
                     /gene="tssK"
                     /locus_tag="ACHK3A_003550"
     CDS             2387..3724
                     /gene="tssK"
                     /locus_tag="ACHK3A_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO8741588.1"
                     /translation="MKICRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     SNVLFDRDALTLGKLKTQAVRLRFADGTLIDSDVADILPPACDLRTLEGGSAIVLLAL
                     PLAHGNGGNLGQGEQAERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLTLNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLQEEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRS"
     gene            3721..4386
                     /gene="tssL"
                     /locus_tag="ACHK3A_003551"
     CDS             3721..4386
                     /gene="tssL"
                     /locus_tag="ACHK3A_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO8741589.1"
                     /translation="MKKETDINIDVLLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4399..6051
                     /locus_tag="ACHK3A_003552"
     CDS             4399..6051
                     /locus_tag="ACHK3A_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8741590.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWWVFVSPSGVLRWAGAAVIVLAVA
                     ALLIYRRRQTWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYTVMPGMYRDTAVLTGQLRRFAHSMAIVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRTIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPLGRAVNRRVEISLVPRSDACQDVK"
     gene            6109..6600
                     /locus_tag="ACHK3A_003553"
     CDS             6109..6600
                     /locus_tag="ACHK3A_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO8741591.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVPTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6792..9437
                     /gene="tssH"
                     /locus_tag="ACHK3A_003554"
                     /gene_synonym="clpV"
     CDS             6792..9437
                     /gene="tssH"
                     /locus_tag="ACHK3A_003554"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO8741592.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLHPLLDAQSDECPA
                     RQPQVTEPLTSAQPESATTDVPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRIAEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAAQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     VQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLKD
                     EQSDLLSMEQSLENRVVGQRPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            9449..11938
                     /locus_tag="ACHK3A_003555"
     CDS             9449..11938
                     /locus_tag="ACHK3A_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO8741593.1"
                     /translation="MGIQNFDHAHHKLKIRGLKSPVDVLTFTGREQLSAPFRYDIEFT
                     STDKTIEPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGVVRAKKGIFISADAQDNAQGQVREMAP
                     AMAILNGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLNQAAILLSAPKGVAI
                     ASGEHLQLAAAKNVIANAGNNADVGAVKNMFIGVGQALSVFVRKAGIKLFANKGAISV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTQGEYNV
                     KAGYYGRKPKAKLTPELMAFPVISAKFDEQVRVIDDDGKFIANVPYYIEDSIGNIYKG
                     FTDSAGCCERIFTEEADNLNVLLGIKALEKW"
     gene            11932..12822
                     /locus_tag="ACHK3A_003556"
     CDS             11932..12822
                     /locus_tag="ACHK3A_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HFO8741594.1"
                     /translation="MVISENTVGTNDIPDSNHDILGPLMPARGFFFIVIDEPGKDGFL
                     VRKIYASPNSPYLTKMNLDPLRLLSETEPERYGLMPKDYSAPTTVAEHVMGNNKSSLI
                     STSSIFPDGSPRFEGKTIFIDVHKAKNAGLKLISTAEIITALEEYKKQYPHLAKRVEK
                     ISGYVDDIDKEVLIEGRKVPAQTIFTPESLKTTQNIVKAGRVVQVFSIFFTAYDLEQA
                     TEKSFETKSIKPIGAEAIRQIGGWGGAVAGAKIGTVIGASLGVTTGPGAVATGIIGGI
                     VFGAAGYFGADWVADYIDEN"
     gene            12812..13429
                     /locus_tag="ACHK3A_003557"
     CDS             12812..13429
                     /locus_tag="ACHK3A_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741595.1"
                     /translation="MKTNWIEHMKNLLVKKPEVKDFFSKDKFPLNLVDGSYPIFISLK
                     DRGIYAAHSIFICSDDELHAIKEHLSNIFNVNKDTIGNEGVIYDSGIVHHPLFRDAIE
                     IQKTYSLIYTFTKSFKGNLIDLVTNSSFFVETIKSAGIKEPVPWDVFPELEPDTFGSL
                     QGELEFWWESIWSPFWHSLSPDERNDFLVKNNATPQWREFIEFHC"
     gene            13477..15963
                     /locus_tag="ACHK3A_003558"
     CDS             13477..15963
                     /locus_tag="ACHK3A_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO8741596.1"
                     /translation="MSIPALNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQ
                     FTSTDKAITPELVLMQDGAFSLTAPPVQGMPVQAPLRTLYGVITGFKHLSSSQDEARY
                     EVRLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQV
                     MQYGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYA
                     DNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPAFIPRPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAVILLSAPKGV
                     AIASGEHLQLAAAKNVIANAGNNADVGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAKKEIIITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIEGGDYSRCFKFFDDENIPYRNVKYIANFIDGTK
                     KEGVTDDEGCTETFFSDKEEEIEIEILLGV"
     gene            15996..>16689
                     /locus_tag="ACHK3A_003559"
     CDS             15996..>16689
                     /locus_tag="ACHK3A_003559"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFO8741597.1"
                     /translation="MSKNVILSNGERENPSPLIYDKHKKHCIFRTKTLLSEEKKEDEK
                     CTVSVVFIDHEGYIRNAGIIKHTVPELEEGRMPKINAIILHRTDSSTINGTLASFSKG
                     IGTHFIVEKDGNIYQAASLFVYTSHVGKIKSRCKEEKTWSSEEKKKTEAFGWNAKKIH
                     DHEKIKQYPYRYPYNMDSVGIEVIALYDKKNKKWESATDSQKKSIEKLVDIIKNIYEL
                     EDRDVYEHDKISY"
BASE COUNT         4211 a         4281 c         4642 g         3555 t
ORIGIN      
        1 tcttaaaagt gcaaatttgt gaagcttacc ctgttattat tttaagtttc atgccaaatt
       61 ctctcaccag ataatgccac cctctcccga aaaataatca agaggccaaa caatatctaa
      121 aatgatacaa ctgtatctat tcccctgaaa aatacattat tcatttgtat attttcctca
      181 tcattgcttt ttatttaaat catccgataa tcccctgaat ataattatgt caataaccat
      241 cagaaaaagt ggatgatgag gaaaaggata tggccgacag tttccagaat gaagttcccg
      301 ccgctcgtgt aaatatcaag cttgatctgc atacaggcaa tgctaaaaag aaagttgaac
      361 tccccctcaa gcttcttgcc gtaggcgatt acagtaacgg aaaagagcaa cgtccgctgt
      421 ccgaacggga caaggttgat atcaataaaa acaacttcaa cagcgtcatg gctgagtttt
      481 cgcctgcggt taatttaaca gtagaagata cgctaaacgg aaacggtaat gaacaaaata
      541 tcgcgcttga atttaaaagc ctgaaggatt ttgaacccga acaggtagca aaaaatattc
      601 cgcaacttcg tgtattactc gctatgcgca atttactacg tgacctgaaa tccaatctgc
      661 tggataacgc aacgtttcgc cgtgaactgg aaaatatcct gaaagacccg acgctcagtt
      721 cagaactgcg tgatgaactg gcgaaaattg cgccgcagga gaacgtttaa cccgaacgcg
      781 tattcaggtt aaaccggaga agaaaggatc cctaaaaagg aaatgctgat gtctgtacaa
      841 caagaatatg ccacctctga aactgcaaca ctcaccacca ctgagtccgg cggcgtttat
      901 cagtccctgt ttgataaaat taatttaacc ccggtctctt ccattcagga aatcgattta
      961 tggcaaaaca gcgaaacgct ggccgatgct tcacccgatg agcgcgtgac ggcggcgatc
     1021 cacgttctgc tttcctgtct ggcgaaatca ggcgaggacg tggttaaact cgacaagagc
     1081 ctgctggatt ttcatatcga cgatttggat cagaaaatca gtaaacagct tgatgcagtc
     1141 atgcaccatc cggaattcca gaaagtcgag tcgctctggc gcggcacatg gttcgtcgta
     1201 cagcgcaccg attttcgcaa aaatgtcaaa attgaactgc tggatatcag taaagaacat
     1261 ctgcggcagg actttgatga ttctccggaa atcattcaaa gtggtttata tcgccataca
     1321 tacattcagg aatatgatac gccgggtggc gaacctgtag cctcattaat atccagctat
     1381 gaattcgata acagcccgca ggatattgcc ctgctgcgta atatttccag agtctctgct
     1441 gcatcacata tgccctttat cggttccgtc ggaccgaagt tcttccttaa aaactcgatg
     1501 gaagaagtcg ccgcaattaa agatatcggc aactattttg accgcgcgga atatatcaaa
     1561 tggaagtcgt tccgcgatac cgacgacagc cgctacgtgg gattagtgat gccgcgcgtg
     1621 ctgggccgtc tgccctatgg cccggacacg gtgccggtac gcagctttaa ctatgtggaa
     1681 gaggtcaaag gcccggatca cgaaaagtac ctctggacaa acgcctcgtt tgcctttgcc
     1741 gccaatatgg tgaagagttt tgtgaataat ggctggtgtg tgcagatccg tggtccgcag
     1801 gcgggtggcg cagtagccga tctgccgatc catctttacg atctcggcac cggcaatcag
     1861 gtcaaaattc cgtccgaagt gatgatcccg gaaacccgcg agtttgagtt cgccaacctt
     1921 ggctttatcc cgctctctta ttataagaat cgcgattacg cctgcttctt ctcagcgaac
     1981 tctgcccaga aaccggcgct gtatgatacc gctgacgcca ccgccaacag ccgtatcaac
     2041 gcccgtttgc cctatatctt cctgctgtcc cgcattgctc actacctgaa aatcattcag
     2101 cgcgagaata tcggcaccac caaagacaga cgcgtgctgg aactggagct gaacacctgg
     2161 atccgcacgc tggtgacgga aatgaccgat ccgggcgatg aacttcaggc ttcgcatcca
     2221 ctgcgcgacg ggaaagttat cgtcgaggac atagaggaca atccgggctt tttccgcgtc
     2281 agactctttg ccgtgccgca tttccagatc gaagggatgg acgtcaacct ttctctggtt
     2341 tcccagatgc caaaagcaaa agcctgatcg ggcggtggtg tgatgtatga aaatttgtcg
     2401 accactgtgg gctgagggga cattcctgtc ttctcagcag tttcagcagc aggcccgctg
     2461 ggaagcgttc agcaatgaca gcattgcgca gctttgtatt cgccatccgt gggggatctc
     2521 aaacgtgctc tttgaccggg atgccctgac gctcggaaag ctgaaaacgc aggcagtacg
     2581 cctgcgtttt gctgatggta cgctgatcga ttcagacgtg gcggatattc tgccgcctgc
     2641 ctgtgacctg cgcacgctgg agggcggcag cgcgattgtt ttgctggcgc tgccgcttgc
     2701 gcatggtaac ggcggtaatc tcggtcaggg cgagcaggct gaaaggccgc tgcgctatcg
     2761 tcaggaatgg caaaaggtgc aggatatcta cggcagtgac agtgaagata tggccgttga
     2821 gcgccatgca ctgtccctgc gctttgccca tgataacaat caggattaca tcacctgtcc
     2881 gctggcgcgt ctggtgcggg atgttcaggg caactggacg caggatgaga gctatattcc
     2941 gccgttactg acgttaaacg cccatgacgg gctggtacag cgcctggaca cgctgctgtt
     3001 gcagctacgg gcgaagtgcc agcgcctgat ggcgatgcgc cgggaaagca accagcgcat
     3061 ggcggacttc gccgtcgccg atgtctctct gttctggctg cttaacgccc tgaattccgc
     3121 cgagccggtg ctgagcgatt tcctgcgcta cccggctgtc caccccgaac tggtctggcg
     3181 ggagctggcg aggcttgccg gtgcgctgct gacgttttcc cttgagcata acgtcagcgc
     3241 cgttccgccc tatgtccacg aatcgccgtc caccgtcttt ccgccactgt tctccctgct
     3301 cagcgagctg ctggaagcca gcctgccgtc gcgggtgatc gcgctggatc tggcgtccct
     3361 gccgggcaac cgctggaaag ccgacctgca cgatccgcgc ctgcaggaag aagccgattt
     3421 ttacctgtcc gttcgttcct cactgcctgc gcatcaggta ctgcaccagc taccgctggt
     3481 ctgcaaaatc ggtgcgcctg atgacgtcac gctgcttatc aacgtggcgc tgaacggtgt
     3541 tccactggtg ccactcacct cggttccggc ggcgctgccg ctgcggctgg agaaccagta
     3601 ttttgcgctg gatatgcaca gtgacgcggc taaatcgatg ctggagtccg ggtgctgcat
     3661 gatttatgcg ccgggaacaa tgggcgacct gaaacctgaa ctgtttgcgg tgctgcgctc
     3721 atgaaaaaag agacggatat caatatcgac gtgctgctgc gcgacacgtt tctgacggtg
     3781 gtggaactgc gtcagggaac caccgtgcgt cacggtatcg agctgtatcg ccactgtcag
     3841 cggcaggtgg aactggtccg cgaacgtctg aaagatgccg gattcagtcg ggagaacgtg
     3901 gagcacataa cttacgcgca gtgcgccctg ctggacgaaa cggtgctcag ccgtagtggc
     3961 atggacgacg gtcaggccat ctggatgaaa aacccactcc agtcgcattt cttcaacact
     4021 ctgcaggcag gcgagctgct gtacgaacgg atgaagcagg tgttgcagga gcccgccccc
     4081 gcgcaggcag tgctgacctg ctttcaccgt gtcctgctgc tgggatttcg cgggcgttat
     4141 caggatccgg tggccccgga gcgtgaccag cttatttcga cgctcaacgg gctggtcgcg
     4201 ccatttggcg tgctgccgga aacggcggta ctgaacgtgc cgttatcccc ccgtcagcat
     4261 cctttgctgc attcgccgtt tttctggctg gtaacgctga cgctgctact ggccggtgtc
     4321 tggtggggac tgcaccactg gctgaacgtg ctggtggatg aattgctgcc gcaaagcctg
     4381 cggtaaaggg agccgtgagt gaggaacacg ctgaaacagg ccatcgtgct gtggggaatg
     4441 gtgttactgc tggtgctgtg gtgggtgttt gtcagtccgt ctggcgtact gagatgggcc
     4501 ggtgcagcag ttatcgttct ggcggttgcc gcattgttga tttaccggcg caggcagacg
     4561 tggacggaga tgaccggcga tgccgggttg ttatcgctgc cgccggaaac gtaccgacaa
     4621 ccggtagtgc tggtctgtgg cgatatgtca gcgcatctgt tcactgacag cccggtccgg
     4681 caggtttcag aagggctgta tctgcatgtt cccgatgaag aacagcttgt ggcgcaggtg
     4741 gagcgattac tgacccttcg cccggcgtgg gcatcgcagc ttgccgtggc gtataccgtc
     4801 atgcccggca tgtaccggga tacggcggtt ctgaccggac aactgcgacg gttcgctcac
     4861 agtatggcga tagtgcgtcg ccgggcgggc gtaaacgtac cctggcttct ctggagcggg
     4921 ctgtccggct cgccgttgcc ggagagagcg aattcaccgt ggtttatctg taccggcggc
     4981 gaagttcagg tagcaacatc cgcagagacc accatgcccg cgcagtggat tgcacaatcc
     5041 ggcgcacagg aacgcagtca gcgactctct tatctgctga aagccgaaag cctgatgcaa
     5101 tggctggatc tgaatgtgct ggcggaactg aacggcccgg aggcgaaatg tccaccactg
     5161 gcgatggccg tggggctggt cccctcgttg cctgcggtgg ataacaacct atggcagttg
     5221 tggatcaccg ccagaaccgg cctgacgccg gatatcgcgg acaccggcac agacgatgcg
     5281 ctgccattcc cggatgccct gttacggcgg ttgccgcgtc agtcgggctt taccccgctg
     5341 cgacgggcct gcgtgaccat gctgggcgtc accaccgtgg cgggtatcgc cgcgctgtgc
     5401 ctgtcagcca cggcaaatcg ccagttatta cggcaggtcg gtgacgatct gcaccggttt
     5461 tatgccgtcc cggtggagga atttatcacc aaagcccgtc acctgtcggt gctgaaagac
     5521 gatgcgacca tgctcgatgg gtattaccgg gaaggagaac cactgcgcct cggtctgggg
     5581 ttataccccg gcgaacgcat ccgccagccg gtattacgca ccattcgcga ctggcgtccg
     5641 cctgaacaaa aaatggaggt gacggcttcg cttcaggttc agaccgtgcg tcttgacagt
     5701 atgtcgctgt ttgacgtcgg acaggcccgc ctgaaagacg gctcgacaaa agtgctggtg
     5761 gacgcactgg tgaacatccg ggcaaaaccg ggctggctga tcctcgtggc cggatatacc
     5821 gacgccaccg gcgacgaaaa aagcaatcag cagttatcgc tgcgacgtgc cgaagcggtg
     5881 cgtaactgga tgctgcagac cagcgacatc ccggccacct gttttgccgt acagggactg
     5941 ggtgagagcc agcctgcggc gaccaacgat acgccactgg gcagggcagt caaccggcgc
     6001 gtcgaaatca gtcttgttcc gcgttctgac gcctgtcagg acgtgaaata aaacataccg
     6061 ccggaagaag gcggtgcttc aatcacacta acaaggagag taattctcat ggctattcct
     6121 gcttatctct ggctgaaaga tgacggcggc gcagatatca aaggttccgt ggacgttcag
     6181 gggcgcgaag gcagcatcga agtggtggcg ttggatcacg atgtgtacat cccgaccgac
     6241 aacaacaccg gcaaactgac cggtacccgt actcacaagc cctttacgtt taccaaagaa
     6301 atcgatgcgt ccagtccgta tctctacaaa gctgtgccca ccggtcagac cctgaaaacg
     6361 gcagaattta agttttaccg catcaacgat gccggtcagg aagtggagta cttcaacatc
     6421 acgcttgata acgtcaagct ggtcagagtc gctccgctga tgcacgacat caaggatcct
     6481 tccagagaga agcataacca cctggaacgt attgagttcc gctacgagaa aatcacctgg
     6541 acttacaaag acggcaacat cattcattcc gactcgtgga atgagcgtcc ttccgcctga
     6601 taactgcggc agacaggaaa ctgtctgcct ttttgttgtg tgctttgtcc agtgccgcag
     6661 gcaccggtac tgagcaaagc atacccacaa tctggtgaaa cctgatgggc ctgtgtgagg
     6721 taacggctat gggcggtagc gtgcctgaac gcggatcgca ggaatacaga acacgataag
     6781 gaaacccttc tatgacagga aatcaccccg ccgcgctgct gcgtcgcctt aacccatact
     6841 gtgcacgggc gttggacgct gccgcctcac tgtgtcagac ccgcgcccat gcggagataa
     6901 ccattgaaca ctggctgctg aaactgctgg agcagggaga aggcgatatc acggtgattg
     6961 cccgtcgcta tgaatgggat atcgacacgc tctggcagtc tctgctggcg catctggaca
     7021 ccttaccccg ctcggtccgc gaacgtcctc aactttctga accgctggca gcgcttatca
     7081 gacaggcgtg gctgatagcg tcactggaag gtgacgatcc acaaatccgc agccagcacc
     7141 tgctgatggc gctgacagaa aaaccgatgc tgcccgcctg taatgacctg tgggtattgc
     7201 tgagtctgag ccgcgtgcag cttgagcggc tgcatcccct gctggatgcg cagtcggatg
     7261 aatgtccggc acgtcagcca caggtcaccg aaccgctgac atccgcgcaa ccagagtcgg
     7321 caacgacgga cgtaccggca aaaacgctga cggggaaaca ggatgacgcc ctgctggcgg
     7381 tgcttaaccg ctttaccgaa gacgtgacgg aaaaagcccg tagcgggcga atcgacccag
     7441 tattcgggcg cgacaccgaa attcgccaga tggtcgatat cctctcccgt cgccgcaaaa
     7501 acaacccgat tctggtggga gaaccggggg tgggcaaaac cgcactggtg gaagggctgg
     7561 cgctgcgtat cgccgaaggc aacgtgcccg acagcctgaa aacggtgcat atccgcacgc
     7621 tggacctcgg tctgttacag gctggcgcgg gtgttaaagg tgaatttgag cagcgactga
     7681 aaaacgtgat tgatgcagca cagaaatcgc cggagccggt actgctgttt attgatgaag
     7741 cccataccat tatcggcgcg ggtaaccagg ctggcggcgc ggatgcggcg aacctgctga
     7801 aaccggcact ggcaaggggc gaactgcgca ccatcgcggc gaccacgtgg agcgaataca
     7861 aacagtattt tgagcgcgac gccgcgctgg agcgccgctt ccagatggtt aaggttgacg
     7921 agccggatga tgacaccgcc tgtctgatgc tgcggggact gaaggcccgt tatgcacagc
     7981 accacggcgt gcatatgctg gacagcgcca ttcagaccgc cgtgcgcctg tcgcgccgct
     8041 atctgaccgg acgccagctg ccggacaagg cggttgattt gctggatacc gccggagcaa
     8101 gagtccgcat gagcctcgac accctgccgg agccgctgac gcagcttcat gcgcgactgg
     8161 cggcgctgga tatcgagcgg gaggcgattg agcaggacag cgtgttttat cccgaaacca
     8221 gcccggagcg gctggcagaa ctgaccgatt tgcgtgatga gctgcaggca gaagccgggc
     8281 atctggaagc gcagtatcag caggaaaagg cactggtgca gcagattatg acgttgcgtc
     8341 aggaaggaac agacagctct gaactgcaac agcaactgcg gacgcatcag ggctttgcac
     8401 cgctgctggc gctggatgtg gacgcccgcg ccgtcgccac ggtggtggcg gactggactg
     8461 gcatcccgct ctcttccctg ctcaaggacg agcagagcga cctgctcagt atggaacaga
     8521 gccttgaaaa ccgcgttgtc gggcaacgcc cggcgctctg cgccatcgca cagcggttgc
     8581 gggcggctaa aaccggcctc acgccggaga acggcccaca gggggtattc ctgctgaccg
     8641 gccccagcgg caccggtaaa accgaaactg cgctcacact ggccgacacc ctgtttggcg
     8701 gtgaaaaatc tcttatcacc atcaacctct ccgaatatca ggagccgcat accgtctccc
     8761 agcttaaggg gtctcctccg ggctacgttg gctacggtca gggcggcgta cttaccgaag
     8821 ccgttcgcaa acgcccgtac agcgtggtgt tgctcgacga agtggaaaag gcgcaccgcg
     8881 acgtaatgaa cctgttctat caggtatttg accggggttt tatgcgcgac ggcgaaggcc
     8941 gggaaattga cttccgcaac accgtgattc tgatgacggc taatctgggc agcgaccaca
     9001 tcatgcaact gctggaggaa aaaccggacg ccacggacgc agacctgcat gaactgctgt
     9061 atcccctgct gcgcgagcac ttccagcctg cactgatggc gcgttttcag acggtgattt
     9121 accgcccgct gggacaggag gcgatgcgca ccattgtgga aatgaaactg gcgcaggtgg
     9181 ttcgtcgtct tcatcagcac tatgggctgg aaacggaaat caacgacagc ctgtatgacg
     9241 cgctgaccgc cgcctgcctg ctgccggaca ccggtgcacg caatatcgac agcctgttga
     9301 accagcaaat cctgccggta ctaagtcagc agttgctggc acagcaggcc gcgcaccgta
     9361 aaccagccca actacggctt ggctgggatg aggaagacgg gattgtactg gagttcgcta
     9421 cggaagagat gcaataacgg aggcttttat gggtattcag aatttcgacc acgcacacca
     9481 caagctcaaa attcgcggcc tgaaaagccc ggtggacgtg ctgacgttta cgggccggga
     9541 acaactcagc gcacccttcc gctacgacat tgaatttacc agtaccgata aaaccatcga
     9601 gccggagtcg gtgctgatgc aggatggcgc attcagcctg acggcaccgc cggtacaggg
     9661 aatgccggta cagactgccc tgcgcaccct gcacggcgtc attaccggct ttaaacatct
     9721 ctcatcgtca caggacgaag cccgttacga agtgcgcctt gaaccccgta tggcgctgct
     9781 tacccgcagc cgccagaacg ccatctacca gaaccagacg gtgccgcaaa tcgtcgaaaa
     9841 aatcctgcgc gagcggcacc agatgcgcgg tcaggacttc gtgtttaacc tcaaaagcga
     9901 atacccgtcc cgtgaacagg tgatgcagta cggtgaggac gacctgacgt ttatttcacg
     9961 cctgctgtca gaagtgggta tctggttccg ctttgccacc gacgcccggt tgaaaatcga
    10021 agttatcgaa ttttacgacg accagagcgg ctacgagcgc gggctgacgc tgccgcttcg
    10081 ccatccttct gggctgtttg atggtgaaac ggaagccgta tgggggctga acaccgcgta
    10141 cagcgtggtg gaaaaaagtg tctccacgcg ggactacaac taccgtgaag ccacggcgga
    10201 aatgaccaca ggccagcatg acgccaccgg gggcgataac accacttacg gcgaagccta
    10261 ccactatgcc gacaacttcc tgcaacaagg cgataaagag gcagcggaaa gcggcgcgtt
    10321 ttatgcccgt atccgtcacg aacgctacct gaatgaacag gccattctca aaggccagag
    10381 caccagttca ctgctgatgc cggggctgga gataaaagtt cagggcgacg acgcgcctgc
    10441 ggtgttccgc aagggcgtgc tgattaccgg cgtgacgacc agcgcggcgc gtgaccgcag
    10501 ctatgagctg acgttcacgg cgataccgta ctcagagcgc tacggctacc gtccggcgct
    10561 tatcccccgc cccgtaatgg ccggaacgct cccggcgcgg gtcaccagta cggtgaagaa
    10621 cgacatctac gcccatatcg acaaggacgg gcgctaccgg gtcaacctcg actttgaccg
    10681 cgatacgtgg aaaccgggct atgagagcct gtgggtacgc cagtcgcgcc cgtatgcggg
    10741 agacacctac ggcctgcacc tgccgctgct ggcgggaacg gaagtcagta tcgcctttga
    10801 ggaaggcaac ccggaccgac cttatattgc aggcgtaaaa cacgactcgg cacacactga
    10861 ccatgtgacc attcagaact ataagcgtaa cgtcctcaga accccggcga acaacaaaat
    10921 ccgcctcgac gatgagcgcg gcaaagaaca tatcaaggtc agtaccgagt acggcggcaa
    10981 gagccagcta aacctcggtc atctggtgga tgccgggaaa cagcaacgcg gcgaaggctt
    11041 tgaactgcgt accgatatgt ggggcgtggt gcgggcaaag aaaggcatct ttatcagcgc
    11101 cgatgcgcag gataatgcgc aggggcaggt gcgcgaaatg gctcctgcaa tggcgatact
    11161 gaatggcgca cagtcacaga tgaagtctct gtccactgat gcgcagacgg cgaatgccga
    11221 tccggcagat ctgtcatcgc agattgcgtt gttgcaacag agcgtaaaag atctgaacca
    11281 ggctgcgatc ctgctaagcg cacccaaggg cgtggcgatc gccagcggcg agcacctgca
    11341 actggcggcg gcaaagaatg tgatagccaa cgcaggcaac aacgccgatg tcggcgcggt
    11401 gaaaaatatg tttatcggcg tgggacaggc gctaagcgtg tttgtgcgta aagcgggcat
    11461 caagctgttt gccaacaagg gggcgatctc ggtacaggca cagaacgatc tgatggaact
    11521 gttggcacag aaatcgatag agatcaccag tacggaagat gagatcaaga tcaccgcgaa
    11581 gaagaaaatc acccttaacg gcggtggcag ctacatccgg ctggatgcct gtgggataga
    11641 agcgggaacg cagggagaat ataacgttaa ggccggatat tacgggcgta aaccaaaggc
    11701 gaagctgacg ccggagctga tggcgtttcc tgtaatatct gcgaaatttg atgagcaagt
    11761 tagagtaatt gatgatgacg gaaaattcat tgctaatgta ccttactata tagaagacag
    11821 catagggaat atctataagg ggtttactga ttctgccggg tgctgtgaaa gaattttcac
    11881 ggaagaagct gataacctaa acgtacttct tggtattaag gcgctggaaa aatggtgata
    11941 tctgagaata cagtaggaac aaatgatata cctgattcaa atcacgatat attaggaccc
    12001 ttaatgcctg caagaggttt cttttttata gttatcgatg agccgggaaa agatggcttt
    12061 cttgtcagaa aaatatatgc ttcaccaaat agcccctacc tgacaaagat gaatctggat
    12121 cctcttagat tattatcaga aacagagcct gaacgctatg ggttgatgcc aaaagattat
    12181 tcagcaccca ctactgtagc agaacatgtt atggggaaca acaaatcctc gttgatttct
    12241 acaagctcta tatttcctga tggctctccg agatttgagg ggaaaaccat atttattgat
    12301 gttcacaaag caaaaaatgc cgggttgaag ttaatctcga ccgcagagat tattactgct
    12361 cttgaggagt ataagaaaca ataccctcat ttggctaagc gtgttgaaaa aatttcaggc
    12421 tatgttgatg atattgataa agaggtcttg atcgaaggta gaaaagtacc agcacaaaca
    12481 atttttacac cagagtctct aaaaacgact caaaacattg ttaaggccgg gcgtgtcgta
    12541 caagttttta gtattttttt cactgcatat gatttagaac aggcaactga aaaatcgttt
    12601 gaaacaaaaa gcataaaacc tataggtgca gaagcaataa ggcaaattgg aggatgggga
    12661 ggagcagtag ctggtgctaa aattggaaca gtgataggcg cgtccctggg tgttactact
    12721 gggccaggag cagtggctac cgggattatt ggcgggattg tttttggtgc agctggttat
    12781 tttggtgcag attgggtagc ggattatata gatgaaaact aattggatag agcacatgaa
    12841 gaatttgctc gttaaaaaac ctgaagttaa agattttttt tcaaaagata aattcccact
    12901 caatttagtt gatgggtctt atccaatatt tatttcactg aaagatcgag ggatatacgc
    12961 tgcgcactca atatttattt gctcggatga tgagcttcat gcaataaagg aacatttatc
    13021 taatatattt aatgtaaata aagatactat cggaaatgaa ggggtcattt acgatagtgg
    13081 cattgtacac caccccctct ttagagatgc tattgagata caaaaaacat actctttaat
    13141 ttacacattc actaaatcat tcaaagggaa tttgattgat ttagtgacga actcttcttt
    13201 ttttgttgaa actattaaat ctgctggaat aaaagaacct gtaccatggg atgtttttcc
    13261 tgagttggaa cccgatactt tcggtagctt acaaggagaa cttgagttct ggtgggagag
    13321 tatctggtct ccgttctggc acagtctatc acctgatgaa agaaacgatt tcttagtgaa
    13381 aaataatgcg acaccacaat ggcgagagtt cattgagttt cattgttgat ttatttttaa
    13441 tgacgaaaat atttctatct atacagggaa ataatgatga gtattcctgc cctaaacttt
    13501 gaccattcac accacaaact caaaatccgc ggcctgcaaa gtccggtgga tgtgctgacc
    13561 ttcgaaggcc gcgaacaact gagtaccccg ttccgctacg acattcagtt taccagtacc
    13621 gataaagcca tcacgccgga gttggtgctg atgcaggacg gcgcattcag cctgacggca
    13681 ccgccggtac agggaatgcc ggtgcaggcc cccctgcgca ccctgtatgg cgtcattacc
    13741 ggctttaaac atctgtcatc gtcacaggac gaagcccgtt acgaagtacg gctggagccg
    13801 cgtatggcgc tgctggcccg cagccgccag aatgccatct accagaatct gacggtgccg
    13861 caaatcgtcg aaaaaatcct gcgcgagcgg caccagatgc gcggtcagga cttcgtgttt
    13921 aacctcaaaa gcgaataccc ggcccgcgaa caggtgatgc agtatggcga ggacgacctg
    13981 acgtttattt cacgcctgct gtcggaagtg ggcatctggt tccgcttcgc caccgacgcc
    14041 cggctgaaaa ttgaggttgt cgagttttat gacgaccaga gcggctatga gcgcgggctg
    14101 acgctgccgc ttcgtcatcc ttcagggctg tttgatggtg aaacggaagc cgtatggggg
    14161 ctgaacaccg cgtacagcgt ggtggaaaaa agcgtcacca cgcgggacta caactaccgc
    14221 accgccacag cggagatgat gacagagcag catgacgcca ccgggggcga taacaccacc
    14281 tacggcgaag cctatcacta cgccgacaac ttcctgcaaa aaggcgataa agaggcagcg
    14341 gaaagcggcg cgttttatgc ccgtatccgc cacgaacgct acctgaatga acaggccatt
    14401 ctcaaaggcc agagcaccag ttcactgctg atgccggggc tggaaatcag ggttcagggc
    14461 gacgacgcgc cagcggtgtt ccgcaagggc gtactgatta ccggcgtgac ggccagcgcg
    14521 gcgcgtgacc gcagctatga actgacgttt accgctatcc cgtactcaga acgctacggc
    14581 taccgcccgg cgtttatccc ccgcccggtc atggccggaa cgctcccggc gcgggtcacc
    14641 agcacggtga agaacgatat ctatgcccat atcgacaaag acgggcgcta ccgggtcaac
    14701 ctcgattttg accgcgatac gtggaaaccg ggttatgaga gcctgtgggt acgccagtcg
    14761 cgcccgtatg cgggagacac ctacggcctg catctgccgc tgctggcggg aacggaggtc
    14821 agcatcgcgt ttgaggaagg caacccggac cgaccgtata tcgccggggt aaagcacgac
    14881 tcggcgcaca ccgaccatgt gaccatccag aactacaagc gtaacgtgct gcgcacccca
    14941 gcaaacaaca aaatccgcct cgacgatgag cgcggcaaag agcatatcaa ggtcagtacc
    15001 gagtacggcg gcaagagcca gctaaacctc ggccatctgg tggatgccgg gaaacagcag
    15061 cgcggcgaag gctttgaact gcgtaccgat atgtggggcg cagtgcgggc gaagaaaggc
    15121 atctttatca gcgccgatgc gcaggacaag gctcagggtc aggtgcggga aatggctccg
    15181 gcaatggcaa tactggatgg cgcacagtca cagatgaagt cgctgtccac tgatgcgcag
    15241 acggcgaatg ccgatccggc agatctgtca tcacagatag cgctgttgca gcagagtgta
    15301 aaagatctga cgcaggcggt gatcctgcta agcgcaccca aaggcgtggc gatcgccagc
    15361 ggcgagcacc tgcaactggc ggcggcaaag aatgtgatag ccaacgcagg caacaacgcc
    15421 gatgtcggcg tggtgaaaaa tatgtttatc ggcgtgggac aggcgctgag cgtgtttgtg
    15481 cgtaaagcgg gcatcaagct gtttgccaat aagggcgcaa tctcggtgca ggcgcagaac
    15541 gacttgatgg aactgttggc gaagaaggaa atcatcatta ccagtaccga agatgaaatt
    15601 aaaatcacgg cgaagaaaaa gatcaccctt aacggcggtg gcagctacat ccggctggat
    15661 gcctgtggga tagaagcggg aacgccggga gaatataacg ttaaggcggg atattatggg
    15721 cgtaagccga aggcgaagct gacgccggag ctgatggcgt ttccggtgat tgagggtgga
    15781 gactattctc gctgttttaa attttttgat gacgaaaata tcccatatcg taatgttaag
    15841 tatatagcca attttattga tggaacaaaa aaggaaggtg tcactgatga tgagggatgc
    15901 accgaaacat tttttagtga taaagaagaa gaaattgaaa ttgagatttt acttggtgtt
    15961 taaaaatacc acccttaatt attaaagaga aaaaaatgag caaaaatgta atattaagta
    16021 atggggaaag agaaaatcct tctccgctga tctatgacaa gcataaaaag cactgtattt
    16081 ttcgtacaaa gacattactt agtgaagaga aaaaagaaga cgaaaaatgt actgtatcag
    16141 ttgtatttat tgatcatgag gggtatataa gaaatgctgg aattataaaa cacacagtcc
    16201 ctgaacttga agaaggtcgc atgcctaaaa ttaatgcaat aatattgcat agaacagatt
    16261 cttcaacaat aaatggtaca cttgctagtt tttctaaagg cattggaact cattttattg
    16321 ttgaaaaaga tggtaatata taccaggctg caagcctatt tgtatatacc agccatgtgg
    16381 ggaagataaa gtctcgatgt aaggaggaaa aaacatggtc gtctgaagaa aaaaagaaaa
    16441 cagaggcatt cggatggaat gctaagaaga tacatgatca tgaaaaaatt aaacaatatc
    16501 cttatagata tccatataat atggacagtg ttggcattga ggttattgca ttatatgata
    16561 aaaaaaataa aaaatgggag tctgctacag actctcaaaa aaaatcaatt gagaaacttg
    16621 tagatataat aaaaaatatt tatgagttag aagataggga tgtttatgaa catgataaaa
    16681 ttagttata
//
LOCUS       DAZGHF010000105        16620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGHF010000105 DAZGHF010000000
VERSION     DAZGHF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.105"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..385
                     /locus_tag="ACHK3A_003560"
     CDS             <1..385
                     /locus_tag="ACHK3A_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjfI family protein"
                     /protein_id="HFO8741598.1"
                     /translation="ALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINILFTSRQM
                     IIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQHEEYYIVFGALSLKSSLE
                     DILLEITSLVDNALDLAEITEEYSH"
     gene            404..1102
                     /locus_tag="ACHK3A_003561"
     CDS             404..1102
                     /locus_tag="ACHK3A_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO8741599.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1152..1811
                     /locus_tag="ACHK3A_003562"
     CDS             1152..1811
                     /locus_tag="ACHK3A_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO8741600.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFLRALGVDIPLTSLHII
                     G"
     gene            1829..2227
                     /locus_tag="ACHK3A_003563"
     CDS             1829..2227
                     /locus_tag="ACHK3A_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO8741601.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2237..2875
                     /locus_tag="ACHK3A_003564"
     CDS             2237..2875
                     /locus_tag="ACHK3A_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO8741602.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2878..4041
                     /locus_tag="ACHK3A_003565"
     CDS             2878..4041
                     /locus_tag="ACHK3A_003565"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO8741603.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4125..5750
                     /locus_tag="ACHK3A_003566"
     CDS             4125..5750
                     /locus_tag="ACHK3A_003566"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO8741604.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERMEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5858..6133)
                     /gene="yjfN"
                     /locus_tag="ACHK3A_003567"
     CDS             complement(5858..6133)
                     /gene="yjfN"
                     /locus_tag="ACHK3A_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO8741605.1"
                     /translation="MKQFLASSSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6282..6611)
                     /gene="bsmA"
                     /locus_tag="ACHK3A_003568"
     CDS             complement(6282..6611)
                     /gene="bsmA"
                     /locus_tag="ACHK3A_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO8741606.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6793..7542
                     /gene="yjfP"
                     /locus_tag="ACHK3A_003569"
     CDS             6793..7542
                     /gene="yjfP"
                     /locus_tag="ACHK3A_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8741607.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHSTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLADRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7539..8294)
                     /gene="ulaR"
                     /locus_tag="ACHK3A_003570"
     CDS             complement(7539..8294)
                     /gene="ulaR"
                     /locus_tag="ACHK3A_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO8741608.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8402..9466)
                     /gene="ulaG"
                     /locus_tag="ACHK3A_003571"
     CDS             complement(8402..9466)
                     /gene="ulaG"
                     /locus_tag="ACHK3A_003571"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO8741609.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9821..11218
                     /gene="ulaA"
                     /locus_tag="ACHK3A_003572"
     CDS             9821..11218
                     /gene="ulaA"
                     /locus_tag="ACHK3A_003572"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO8741610.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKFAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11234..11539
                     /gene="ulaB"
                     /locus_tag="ACHK3A_003573"
     CDS             11234..11539
                     /gene="ulaB"
                     /locus_tag="ACHK3A_003573"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO8741611.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            11549..12013
                     /gene="ulaC"
                     /locus_tag="ACHK3A_003574"
     CDS             11549..12013
                     /gene="ulaC"
                     /locus_tag="ACHK3A_003574"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO8741612.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            12027..12677
                     /gene="ulaD"
                     /locus_tag="ACHK3A_003575"
     CDS             12027..12677
                     /gene="ulaD"
                     /locus_tag="ACHK3A_003575"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO8741613.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12687..13541
                     /gene="ulaE"
                     /locus_tag="ACHK3A_003576"
     CDS             12687..13541
                     /gene="ulaE"
                     /locus_tag="ACHK3A_003576"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO8741614.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEISVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13541..14227
                     /gene="ulaF"
                     /locus_tag="ACHK3A_003577"
     CDS             13541..14227
                     /gene="ulaF"
                     /locus_tag="ACHK3A_003577"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO8741615.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14356..14631)
                     /gene="yjfY"
                     /locus_tag="ACHK3A_003578"
     CDS             complement(14356..14631)
                     /gene="yjfY"
                     /locus_tag="ACHK3A_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO8741616.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14959..15354
                     /gene="rpsF"
                     /locus_tag="ACHK3A_003579"
     CDS             14959..15354
                     /gene="rpsF"
                     /locus_tag="ACHK3A_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO8741617.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15361..15675
                     /gene="priB"
                     /locus_tag="ACHK3A_003580"
     CDS             15361..15675
                     /gene="priB"
                     /locus_tag="ACHK3A_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO8741618.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15680..15907
                     /gene="rpsR"
                     /locus_tag="ACHK3A_003581"
     CDS             15680..15907
                     /gene="rpsR"
                     /locus_tag="ACHK3A_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO8741619.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15949..16398
                     /gene="rplI"
                     /locus_tag="ACHK3A_003582"
     CDS             15949..16398
                     /gene="rplI"
                     /locus_tag="ACHK3A_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO8741620.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         3945 a         4005 c         4475 g         4195 t
ORIGIN      
        1 ggcgctggcg acggcgctgc aaactgtacc tgaacaaaat attgatgtaa caaatagcga
       61 gaacgcatta attattaaaa tgaatgatta tggcgatttg caaatcaata ttctatttac
      121 ttcccgccaa atgattatcg aaacctttat ttgtccggta agtagtatca gcaatccaga
      181 tgaatttaat accttcttat taagaaatca gaagatgatg ccgttgtcat cggtagggat
      241 ctccagcgtg caacatgaag agtattacat tgttttcggt gcgttatcgc ttaaatcttc
      301 tcttgaagat atcctgcttg agataacctc gctggtagat aacgcattgg atctggctga
      361 aattacagaa gaatattcac actaattaca aggacgggta tttatgggaa ttttaaaaag
      421 tttatttacg ctggggaaat cgtttatctc ccaggcggaa gaatccattg aagaaaccca
      481 gggcgtgcgc atgctggaac agcatattcg tgacgctaaa gctgaactcg ataaagccgg
      541 aaaatctcgc gttgatctgc tggcgcgggt gaaattaagt cacgataagc tgaaagattt
      601 acgtgagcgc aaagccagtc tggaagctcg tgcgctggaa gcgttaagca agaacgttaa
      661 tccgtcgttg attaacgaag ttgctgaaga aatcgcgcgc cttgagaatc tcattaccgc
      721 tgaagagcaa gtgttgtcga atctggaagt ctctcgtgat ggcgtggaaa aagcagttac
      781 agcgacagcg cagcgtatcg ctcagtttga gcagcaaatg gaagtcgtta aagctactga
      841 agccatgcag cgtgcacaac aggctgtaac gacctctact gttggcgcat cttccagcgt
      901 ttcgacagcg gcagaatcct taaaacgcct gcaaacgcgt caggccgaac gtcaggctcg
      961 cctggatgct gccgcacagt tggaaaaagt cgcagacggt cgcgatcttg atgaaaagct
     1021 ggcggaagcc ggaattggcg gtagcaataa aagtagcgcc caggatgtat tagcaagact
     1081 gcaacgccaa cagggcgagt aattttttgc cagcctcact gcaaggctgg caactatttt
     1141 aaggataaaa tatgtctggt tttttccagc gtctgtttgg caaggataat aagcctgcta
     1201 tcgctcgcgg tcctctggga cttcatctca atagtggctt tacgctcgac acgttagcgt
     1261 ttcgtttgct ggaagatgaa ttgctgattg cgctgccggg tgaggaattt acggtagccg
     1321 ctgtcagccg catcgatctg ggcggcggta gccagatttt ccgctactac acttcaggcg
     1381 atgagtttct gcaaatcaat accactggcg gcgaagatat tgatgacatt gatgatatca
     1441 agctctttgt ctatgaagag agctacggta tcagtaaaga gagtcactgg cgcgaggcga
     1501 tcaatgccaa agcgatgggg gcaatgacct taaactggca ggaaaaacgc tggcagcgat
     1561 tttttaatag cgaagaaccg ggaaatatcg aacccgtcta catgctggaa aaagtagaaa
     1621 atcaaaacca tgctaaatgg gaggtccata attttaccat gggctatcaa cggcaagtga
     1681 ccgaagatac ttacgaatat ctgctgttaa atggtgagga atcttttaat gatctcggcg
     1741 agccggagtg gttatttttg cgcgcgctgg gggtcgatat cccgctgaca tcacttcata
     1801 ttattggtta attacaagga cgtttacgat gcatatactg gattcacttc ttgcctttag
     1861 cgcttatttt tttattggtg tggctatggt gattattttt ctgtttatct actccaaaat
     1921 tacaccgcac aacgaatggc agttaatcaa aaataataac accgcggcgg cactggcgtt
     1981 cagcggaaca ttgttggggt acgtgatccc cttatccagt gcggcaatca atgcggtgag
     2041 tattccagac tatttcgcat ggggcgggat tgctctggtg attcaattac tcgtttttgc
     2101 tggcgtcagg ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc
     2161 aggaatgttt atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac
     2221 atggtaatgg acgatcatgg ccagaaaacg caaatccaga aataacaaaa atatcggaca
     2281 tggggcaatc agtcgcattg gtagaccgca taatcctttt gagccgcgcc gcaatcgcta
     2341 cgcacaaaaa tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg
     2401 ttgtggcgat agtggcgatg tcgataacga cggcgacgga acgttttacg cgactgtgca
     2461 ggattgtatt gatgacggta ataatgcaga catttgcgcg cgtggctgga acaacgccaa
     2521 agcggcgttt tatgccgatg ttccgaagaa tatgactcag cagaattgtc agtctaagta
     2581 cgaaaattgc tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt
     2641 gttaagccgg gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc
     2701 ctcatttgct tcgcgccccg tctggcgcaa cacttctggt gattactcct ggcgctccgg
     2761 ttctggcaaa aaagagtctt actcttcggg cggctttacc accaaaaaag cgtcaaccgt
     2821 ttctcgcggc ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg
     2881 ctgagacaca acgttcctgt gcggcgggat ctggaccaga tcgccgctga caacggtttc
     2941 gactttcata tcatcgacaa tgaaatctat tgggacgaga gtcgagctta ccgttttact
     3001 ctgcgccaga ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt
     3061 gaggtggtgg atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg
     3121 ttgtactggg atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgtt gtatggccgt
     3181 atggattttg cctggtgtgg taatgcaccg gttaagttgc tggagtacaa cgccgatacg
     3241 ccaacttcat tgtacgagtc ggcttatttc cagtggttgt ggctggaaga tgcccggcgc
     3301 agcggcgtta ttccgcgcga tgccgatcag tacaatgcca ttcaggagcg cctgatttca
     3361 cgctttagcg agctttacag tcgggaaccg ttttattttt gctgctgtca ggacaccgac
     3421 gaagacagga gtaccgtgct gtacttgcag gactgcgccg agcaggcagg gcaggagtcg
     3481 cggtttatct acattgaaga tctcggtttg ggcgtcggcg gtgtgctgac cgatcttgat
     3541 gataatgtca tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat
     3601 aacggtccgc tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc
     3661 ttgagtaata aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt
     3721 cttgcatcct ggttcgatgg tgaaaaaccg cagattgccg ctggcgaaag ctatgtgcgt
     3781 aaaccgattt actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtt
     3841 gttgaccacg ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct
     3901 ctgccgcgat ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg
     3961 tgcggaatgg ggatccgtga agataacacg ttgatcacca aagacacctc acgtttcgtt
     4021 ccgcattaca ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc
     4081 agtgaccata ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc
     4141 acaccgtttt taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg
     4201 cgctctgcga agcggtaacg cgtgaaggtg ctggctggga tagcgatttt ctcgccagta
     4261 ttggtcagca gttagggacg gctgaatccc ttgaactggg gcggctggcg aatgtgaatc
     4321 cgcctgaatt attgcgctac gatgcccagg ggcggcgtct ggacgatgtg cgttttcacc
     4381 ccgcctggca cctgctgatg caggcgctat gtaccaatcg ggtgcacaat cttgcctggg
     4441 aagaagacgc tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttgcacgcac
     4501 aggttgaggc agggtcgtta tgtccgataa cgatgacctt tgccgccacg ccattgctgt
     4561 tacagatgtt acccgcgccg tttcaggact ggaccacgcc gctgttgagc gatcgctacg
     4621 attctcactt attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg
     4681 aaaaacaggg cggttccgat gtcatgagca acaccacccg cgcagagcgc atggaagatg
     4741 gctcttatcg gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc
     4801 tggtgctggc gcagactacg ggcggtttgt cctgcttttt tgtgccgcgc tttttgcctg
     4861 acgggcaacg caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta
     4921 acgccagctg cgaagtggag tttcaggatg ccattggctg gttgttgggg caggaagggg
     4981 aaggaattcg tctgatcctg aaaatgggtg ggatgacgcg ttttgattgc gccctgggta
     5041 gccatgccat gatgcgccgt gcattttcgc tagcgattta tcatgcacat caacgccatg
     5101 tttttggtaa tccattgatc caacagcccc ttatgcgtca tgtcttaagt cgcatggcac
     5161 ttcagcttga agggcaaacg gcgttgctgt ttcgtcttgc tcgagcgtgg gaccggcgtg
     5221 ccgatgccaa agaagccctg tgggcgcgtt tatttacgcc tgcggcgaaa tttgtgatct
     5281 gcaaacgagg tatgccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt
     5341 gcgaggagag cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag
     5401 gttccggtaa tattatgtgc ctggatgtac tgcgcgtgct caataagcaa gcgggtgtat
     5461 acgacttatt gtcggaagca tttgtggaag tgaaagggca ggatcgctat tttgatcgcg
     5521 cggttcgtcg tttacagcag cagctgcgta agccagctga agaactgggg cgagagatta
     5581 ctcatcagct attcctgctg ggctgcggtg cgcaaatgtt gaaatatgcc tctccgccaa
     5641 tggcgcaggc gtggtgtcag gtgatgttag atacgcgcgg cggcgtacgg ttgtcagagc
     5701 agatccagaa tgatttattg ctgcgggcga cgggaggagt gtgtttgtaa gcgtatacga
     5761 cgctggtttc gattaactca atgaaatatg tgaaaattgt tagccggaca aggcgctcgc
     5821 gccgcatccg gcattgttca tttgctgtag atgacactca cgcatacagg atcgcctgta
     5881 cgcgccagtt atcaggctgc tggtcttcgt acatggtaat aatgcggtac catgacgcac
     5941 cttgttcatc agcctttaac gccacgatac gttccacgtc ctgcggatcc cctgaaatat
     6001 tgctaactga gatctgacct atttcattca ggcctgtcac gcagtcagca ctggcgaatt
     6061 ctgcagactg tgccgtggcg ctcgcaggat aagtcactaa ttgcagcgag gatgaggcaa
     6121 ggaattgttt catcgtcagc tccatttttc gtttccccac atgttgcagg gcattccatc
     6181 gcggtaataa acgcccaggc ttccagccgg taatttattg tgcacaggta aacgcatagg
     6241 gacgcaaagc aatgtaaagc tgactatgta agttgtctga tttatttacg gtacaaaatg
     6301 gcttgtgaat accactgtcc tgtgacgatg gtttcgtcca ccatgacgac aacgtaataa
     6361 tcagcttttg cagcgacagc tttcgctctg atttctgcta atgcgtcatc cggagaaccc
     6421 cgaaccatcg tgcttacgct acctattcgc tgtaaccctt gcgtctggtc gcggcgaatc
     6481 tcttgcggat ggtcggttac tggcggtgct ggctgtggcg taccttgtag tgcgctacag
     6541 gcacttaaca tcaacaccaa taataaactg gcaaaccggt aaataacgct attacgtttc
     6601 ctgctaacca tagtgtagtg ccttattaat ttaactttgg gggaatgttc ccgaattgtt
     6661 acctggtacg cgattttcga atgaaaacgt gatgaaagcg taacccacat ctcaacatta
     6721 tgaactaata ccgccagcag aaacttgtgc taaggatttc ttcatgtcat accagacaaa
     6781 aaggagagac agatgattga aatagaatca cgcgagctgg cagatattcc cgttcttcat
     6841 gcttatcctg tcgggcaaaa agataccccg ttaccgtgcg cgatttttta tcacggcttt
     6901 acttcgtcca gtctggtgta tagctatttt gccgttgcgc tggcgcaggc tggtttgcgg
     6961 gtgatcatgc cggatgcgcc cgatcacggt agtcgtttta gtggcgacgc agcgcggcgg
     7021 ttaaatcaat tctggcaaat cttgctacaa agtatgcagg aattcactac tttacgtgcg
     7081 gcaatagccg aagaaaactg gctgcgtgat gaccgtctgg cagtcggtgg cgcgtcgatg
     7141 ggcgcgatga cggcactggg aattaccgct cgccactcca cggtgaaatg taccgccagc
     7201 atgatgggat cgggctattt tacatcgctc gcccgttcac tgtttccacc gctgatacct
     7261 gaaacggcag cacagcagaa tgaattcaat aacattgtcg cgccgctggc agagtgggaa
     7321 gcgacaaacc acctggaaca acttgctgac agacctctac tgctgtggca tggccttgac
     7381 gatgacgtcg tgcctgccga cgaatcacta cgtttgcagc aggccttaag cgagacggga
     7441 cgggataaac tgctaacctg ttcatggcag ccaggcgtgc gtcaccgcat tacgcctgag
     7501 gcgttagatg ctgccgtgac atttttccgc cagcatcttt aaacacgcag aatgctgacc
     7561 ccttgcgctt ccagttgttg caggatttcc gggttagcat ttttgccggt gatcagcata
     7621 tcgatttgat cggcacggct aaaaagcatt cccgcgcgtt cgccgatctt actgctatca
     7681 accagtacca ccagtttccc taccacgctt agcatcttct gctctgccat tgccgtcagc
     7741 atatcggttt tatacaaccc ttctgctgtc agccctttgc cgctggtaaa catccagtgc
     7801 ccggcataga gactgttttc gctgccttgc gggcttaaag tgatggactg acttttgttg
     7861 tactgtccgc ccataatgat cacgctgtca tgctcctgat cgatcaggta atttgccagc
     7921 ggtagataat tagtgatgat ttgcactggc ttgccacaca tttcccgccc aagcagaaac
     7981 gcggtggagc cgcagttgat gactacgctt tcgcccggat taaccagctg cgaggccgct
     8041 ttagcaatac gtactttttc atcgtgattc tgcgcctgat gcagattcat cggcgtccag
     8101 cgtgggcgct gctgggtaat agcttctgcg ccattgcgca cttttttcag tttgccgctt
     8161 tcgtcaagtt tattgatatc gcgtcgcgca gtggcaggcg aaattcccag acgctcaacg
     8221 actttctcaa cggtcacaaa gcccaattgt gcgagcattt ccaggaggat ttgatgtctt
     8281 tgtgcttcag tcatgagcta ttccgataaa atttgatttt tttagatgat atttgaaata
     8341 gccaggaaat actacgccgg atagcgcgaa ctactccacg ctatccggta caagcgacag
     8401 attacaggaa cgacttgaac ggcagatccg gttcaatagt gaagcaatca tcgaaaccac
     8461 gcggatagtg gtactcgaag ttgtctttat ccagcggcca ggtaaatttg ccgccgacct
     8521 gccagataaa cggcttgaag ccatacttca ggcgatcttt tttcatctcc cacagcacgc
     8581 ggatctcttg cggatcggcc tggaagtttg accagatatc gtggtggaac gggatcacta
     8641 ctttagcatt cagcgcttcg cccatacgca agatatcagc actggtcatt ttgtcggtaa
     8701 taccgcgtgg gttttcgccg tatgaaccta aagccacgtc gatctgatgc tcgttgccat
     8761 gttttgcata gtagttagag tagtgggaat cgccgctgtg atacagagaa ccgcccggtg
     8821 ttttgaacag atagtttact gcgcgatcgt ccatgccgtc tggcagcacg ccagccgctt
     8881 tttgatccgc tggtagggtg atcagtgcag tacggtcgaa cgcgtcaagg gcatgaattt
     8941 caatgtcttt cacttttacg acatcgcccg gtttgaccac gatgcaacgc tctttcggta
     9001 cgccccagcc aatccataaa tccacgcagg ttttcggtcc gataaacggt acgtcatcag
     9061 cacagttctg catcacggca gcagcgacgt tgacgtcgat atgatcgttg tgatcgtgag
     9121 ttgccagtac cgcgtcgatc tggcgaatcg caaacggatc aagaacaaac ggggtggtgc
     9181 gcaggtttgg ctgcagtttt ttcacgccag ccatgcgctg catctggtga ccctgtttca
     9241 tcagcgggtt accgtgactt tgtttgccag taccgcacca gaaatcaacg caaacgttgg
     9301 tgccaccttc cgatttcagc cagatcccgg tgcagcctag ccaccacatc gcgaatgtgc
     9361 caggggcgac ctgctcttgt tcaatttctt cattcagcca gctaccccac tccgggaaag
     9421 tgctcaggat ccaggattct cgggtgatac ttttcacttt actcatcgcc atttaccttc
     9481 atgatagttc aattcgaatc aacatgtgat tggttttgat taatcctgac actatttttt
     9541 caggaaggca atgaccattt tttgactttt tgccagggaa gttgttgttg attttgagtg
     9601 tggaaagatt taatggaatg tgtaattcaa ttaactaaat gaatttaaat gaataattat
     9661 ttcgttgtgt gaatcccact ctatccatgt ggaattattt gcgggtcgcg tcacatttaa
     9721 tcataaataa tcttgttgtg attacttttg aaaattagag tgagtgcaca acattccggg
     9781 tgtgtggaat acccggttac ctcttcttca ggagatcgtt atggagatcc tctacaacat
     9841 ctttaccgtg ttttttaacc aggtcatgac caatgccccg ttgttgctgg gtattgtgac
     9901 ctgtctgggc tacatcctac tgcgcaaaag tgtcagcgtt attattaaag gcacgattaa
     9961 aaccataatt ggtttcatgt tgttgcaggc aggatccggc atcctcacca gcaccttcaa
    10021 accggtggtg gcgaaaatgt ccgaagtcta cggcattaac ggcgcaattt ccgatactta
    10081 cgcgtcgatg atggcaacca tcgaccgcat gggcgatgcc tatagctggg taggttacgc
    10141 ggtgttgtta gcgctggcac tgaacatctg ttacgtgctg ctgcgtcgca ttaccggtat
    10201 tcgtaccatc atgctgactg gtcacatcat gttccagcag gcggggctga ttgccgtcac
    10261 gctgttcatc tttggctact ccatgtggac gaccattatc tgcaccgcga ttctggtttc
    10321 gctctactgg ggcattacct ccaacatgat gtacaagccg actcaggaag tgacggacgg
    10381 ctgcggtttc tccatcggtc accagcaaca gtttgcatca tggattgcct ataaatttgc
    10441 gccgttcctc ggcaaaaaag aggaaagcgt tgaagacctc aaactgccag gctggctgaa
    10501 tattttccac gacaacatcg tctccacggc gattgtcatg accatcttct ttggtgccat
    10561 tctgctctcc ttcggtatcg acaccgtgca ggcgatggca ggcaaagtga actggacggt
    10621 gtacatcctg caaactggtt tctccttcgc ggtggcgatc ttcatcatca ctcagggtgt
    10681 gcgcatgttt gtggcggaac tctctgaagc gtttaacggt atctcccagc gtctgatccc
    10741 tggtgcggtt ctggcgattg actgtgcggc tatttatagc ttcgcgccga acgccgtggt
    10801 ctggggcttt atgtggggca ccatcggtca gctgattgcg gttggcatcc tggtcgcctg
    10861 cggttcctcg atcctgatta tcccaggctt tatcccgatg ttcttctcca acgccactat
    10921 tggcgtgttc gctaaccact tcggcggctg gcgtgcggcg ctgaagattt gtctggtgat
    10981 ggggatgatc gaaatctttg gctgcgtctg ggcggtgaaa cttaccggta tgagtgcctg
    11041 gatgggtatg gcggactggt cgattctggc accgccgatg atgcagggct tcttctccat
    11101 cggtatcgcc tttatggctg tcatcattgt aattgcactg gcttatatgt tcttcgctgg
    11161 ccgcgcactg cgcgcagaag aagatgcaga aaaacaactg gcagaacagt ctgcttaata
    11221 aggagttttg attatgaccg tacgtattct ggctgtgtgt ggcaacggac aaggcagttc
    11281 catgatcatg aaaatgaaag tggaccagtt tttaacccag tcgaacattg accacacggt
    11341 aaacagctgc gcggttggcg aatacaaaag cgagttgagc ggcgcggata tcatcatcgc
    11401 ttctacccat attgcgggcg aaatcactgt gaccggcaac aaatatgtgg ttggcgtgcg
    11461 caatatgctc tctcctgccg actttggccc gaaattgctg gaagtgatca aagagcattt
    11521 tccgcaggac gtgaagtaag gacgcgccat gaaattacgt gattcgctgg cggagaataa
    11581 atccatccgc ctgcaggctg aagctgagac atggcaggac gcagtaaaaa tcggcgttga
    11641 cctgctggtg gcggcggatg ttgtcgagcc gcgttactac caggcgattc tggatgcggt
    11701 tgaacagcac ggtccgtact ttgtgctcgc acccggcctg gcaatgccgc acggtcgccc
    11761 ggaagagggc gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt
    11821 caaccacgaa gataacgatc cggtggatat cctcatcacc atggcggcgg tcgatgccaa
    11881 cactcaccag gaagtgggca tcatgcagat cgtcaatctg tttgaagatg aagagaattt
    11941 cgaccgttta cgtgcctgcc gtaccgagca ggaagtactg gatctcattg accgcaccaa
    12001 cgcggcagct taagaaggaa ttgaaaatgt cattaccgat gttgcaggtc gcgctggaca
    12061 accagactat ggatagcgcc tatgaaacca ctcgcctgat tgccgaagaa gtcgacatta
    12121 tcgaagtggg taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgc gatctgaaag
    12181 cgctttaccc gcacaaaatc gtactggcag acgccaaaat tgccgatgcg ggcaaaatcc
    12241 tttctcgtat gtgcttcgaa gctaacgctg actgggtgac cgtgatttgc tgtgcggata
    12301 tcaacacagc taaaggtgcg ctggacgtgg caaaagagtt taacggcgac gtgcagatcg
    12361 aactgaccgg ttactggacc tgggaacagg cgcaacagtg gcgcgacgca ggcattgggc
    12421 aggtggttta tcaccgcagc cgtgacgcac aggccgcagg tgtggcgtgg ggcgaagcgg
    12481 acattaccgc gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc accggtggtc
    12541 tggcgctgga agatctgccg ttgtttaaag gcattccgat tcatgtgttt atcgctggtc
    12601 gcagcattcg tgatgccgct tctccggtgg aggccgcacg tcagttcaaa cgttccatcg
    12661 ctgaactgtg gggctaagga gcggatatgt tgtccaaaca aatcccgctt ggcatctatg
    12721 aaaaagcgct ccccgccggg gagtgctggc tggaacggct gcaactggca aaaacgttag
    12781 gcttcgattt tgtcgagatt tcggtagatg aaacggacga tcgcctgtcg cgcctcgact
    12841 ggagccgcga gcagcgtctg gcgctggtga acgcgattgt tgaaaccggc gtgcgcgtgc
    12901 cgtccatgtg cctttctgct catcgtcgtt tcccgctggg cagcgaagat gacgcagtgc
    12961 gggcgcaggg gctggagatt atgcgcaaag ctatccagtt cgcccaggat gtcggtattc
    13021 gcgtgatcca actggcgggc tacgacgttt actatcagga agccaataac gaaacgcgtc
    13081 gtcgtttccg tgacggcctg aaagagagcg ttgagatggc aagccgcgcg caggtgacgc
    13141 tggcgatgga gatcatggat tacccgttga tgaactccat cagcaaggcg ctgggttacg
    13201 cgcactatct taacaacccg tggttccagc tctacccgga tatcggcaac ttgtcggcgt
    13261 gggacaacga cgtgcagatg gagttgcagg ccgggatcgg gcatatcgtc gcggtgcatg
    13321 tgaaagacac caaaccaggc gtcttcaaaa acgtcccgtt tggcgaaggt gtagtggatt
    13381 tcgaacgttg cttcgaaacg ctcaaacaga gtggttattg cgggccgtac ctgattgaga
    13441 tgtggagcga aacggcggaa gacccggcgg cagaagtggc gaaagcgcgt gattgggtga
    13501 aagcgcgcat ggccaaagcc ggcatggtgg aggcggcata atgcaaaagc taaaacagca
    13561 ggtatttgaa gccaatatgg atctgccgcg ctacgggctg gtgaccttta cctggggcaa
    13621 cgtcagcgcc attgaccgcg aacgcgggct ggtggtgatc aagcccagcg gcgttgccta
    13681 cgaaaccatg aaagcagacg atatggtggt ggttgatatg agcggcaagg tggtggaagg
    13741 ggagtatcgt ccgtcttccg acaccgcgac gcatcttgaa ctctaccgcc gttatccgtc
    13801 gcttggcggc attgtccata cccattccac gcatgccacc gcatgggcgc aggcggggct
    13861 ggctatcccg gcattaggca ccacgcacgc tgactacttc tttggcgata tcccctgtac
    13921 gcgcgggtta agcgaagaag aagtgcaggg ggaatacgag ctaaacactg gcaaagtgat
    13981 tatcgaaacg ctgggtgacg ccgagccgct gcatacgccg ggaattgtgg tgtatcagca
    14041 cgggccattc gcctggggga aagatgctca cgatgcggtg cataacgcgg tggtgatgga
    14101 agaagtggcg aaaatggcgt ggattgcccg cggcattaac ccgcaactga atcacatcga
    14161 cagctatctc atgaataaac acttcatgcg taagcacggt cccaacgcct attacgggca
    14221 gaagtaaaac acgcgctgcg gagatttcct tcttcgggag ataactggtc taagtccgca
    14281 gccgtttttc aaaaaaagcc ccctgaaaag ggggcaaagc aaactatggc aatgtttcgt
    14341 tggttatacc tggtgctagc gataaatatc cgcgctggcg tgcatattgc cgttactccc
    14401 cggttcccgc atcagaatca cgtggtagta cgttgcgccc tgcgcatctg tctcttcatt
    14461 taatgcctga cgcgcttcgc tttcagtggc gaaattatga ttgatataaa tcacgcctaa
    14521 gctttgcaca tcgtccatat ttctggcctg gtggttatta atttcaatgg ctgcccatgt
    14581 atttgcactt agcaaaagca cagccagaag ggctaaaaca cggctgaaca tagatacctc
    14641 ctcgacggct gactttgtgt gctctcctct tgtggtgatc ttctgattta attttaatca
    14701 atgataaaga agtggatggt gaccatttct gatgcagttg ttcaaaaaaa caccatgatg
    14761 aagtgtgatg aacttcaaat cagcgtgtta gtacgatttg gtgaaggatg agatttattt
    14821 gtcggctggc cttgagttta gcggggtatg tcagtactat aacgcgtcat ttttcagccg
    14881 acctttaaca cgttccttgc ctccccggga ttcggctgac ccagacagga ggctgaataa
    14941 tccgtaagga gcaattcgat gcgtcattac gaaatcgttt ttatggtcca tcctgaccag
    15001 agcgaacagg ttccgggcat gatcgagcgc tacactgctg ccatcactgg tgcagaaggc
    15061 aagatccacc gtctggaaga ctggggccgc cgtcagctgg cttacccgat caacaaactg
    15121 cacaaagcac actacgttct gatgaacgtt gaagctccgc aggaagtgat cgatgagctg
    15181 gaaactacct tccgcttcaa cgatgccgtt atccgcagca tggttatgcg taccaagcac
    15241 gctgttaccg aagcatctcc gatggttaaa gcgaaagacg agcgccgtga gcgtcgcgat
    15301 gatttcgcaa acgaaaccgc tgatgatgct gaagctgggg attctgaaga gtaatttctg
    15361 atgaccaacc gtctggtgtt gtccggcacc gtgtgcagga ctccccttcg aaaggtcagt
    15421 ccatcaggaa ttcctcactg ccagttcgtg cttgagcatc gttctgtgca ggaggaagcc
    15481 ggctttcacc ggcaggcatg gtgccaaatg cccgttattg ttagcggaca cgaaaaccag
    15541 gccattactc acagtataac ggtcggcagt cgcataaccg ttcaggggtt catttcatgc
    15601 cataaggcaa agaacggact gagcaaaatg gttttgcatg ccgagcagat tgaattgata
    15661 gattctggag actagccata tggcacgtta tttccgtcgt cgcaagttct gccgtttcac
    15721 cgcggaaggc gttcaagaga tcgactataa agatatcgct acgctgaaaa actacatcac
    15781 cgaaagcggt aagattgtcc caagccgtat caccggtacc cgtgcaaaat accagcgtca
    15841 gctggctcgc gctatcaaac gcgctcgcta cctgtccctg ctgccgtaca ctgatcgcca
    15901 tcagtaatcg gtcacggtcc attaatacga ctttgagagg ataaggtaat gcaagttatt
    15961 ctgcttgata aagtagcaaa cctgggtagc ctgggtgatc aggtaaacgt taaagcgggc
    16021 tatgctcgta acttcctggt accgcagggt aaagctgttc cagctaccaa gaaaaacatt
    16081 gaattcttcg aagcacgtcg cgctgaactg gaagctaaac tggctgaagt tctggcagca
    16141 gctaatgctc gcgctgagaa aatcaatgca ctggaaactg ttaccatcgc gtctaaagct
    16201 ggcgacgaag gtaaactgtt cggttccatc ggtactcgcg acatcgctga cgctgtaact
    16261 gcagctggcg ttgaagtggc taagagcgaa gttcgtctgc cgaacggcgt tctgcgtacc
    16321 actggcgaac acgaagtgag cttccaggtt cacagcgaag tattcgcgaa agtgatcgta
    16381 aacgtagtag ctgaataatt cgttattcaa cgagacgtaa aaacgccgac cagtggtcgg
    16441 cgttttgctt ttaagtgata aaaaccgcag aaacagacgt tccctccata aatctcttgc
    16501 tattcctttt ataaaaagtt cgcagtaccc atacctggcc cccagcctcg ccgctggtag
    16561 ttcgtgtcat gttttttacg cgtaaaacac gacaaaagca tcaatacagc gatcgtctca
//
LOCUS       DAZGHF010000106        16324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGHF010000106 DAZGHF010000000
VERSION     DAZGHF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.106"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(40..759)
                     /gene="dcuR"
                     /locus_tag="ACHK3A_003583"
     CDS             complement(40..759)
                     /gene="dcuR"
                     /locus_tag="ACHK3A_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO8741621.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(756..2387)
                     /gene="dcuS"
                     /locus_tag="ACHK3A_003584"
     CDS             complement(756..2387)
                     /gene="dcuS"
                     /locus_tag="ACHK3A_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO8741622.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2567..2797
                     /gene="yjdI"
                     /locus_tag="ACHK3A_003585"
     CDS             2567..2797
                     /gene="yjdI"
                     /locus_tag="ACHK3A_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO8741623.1"
                     /translation="MDQALLDRGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2809..3081
                     /gene="yjdJ"
                     /locus_tag="ACHK3A_003586"
     CDS             2809..3081
                     /gene="yjdJ"
                     /locus_tag="ACHK3A_003586"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8741624.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3308..3604
                     /gene="ghoS"
                     /locus_tag="ACHK3A_003587"
     CDS             3308..3604
                     /gene="ghoS"
                     /locus_tag="ACHK3A_003587"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO8741625.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3632..3805
                     /gene="ghoT"
                     /locus_tag="ACHK3A_003588"
     CDS             3632..3805
                     /gene="ghoT"
                     /locus_tag="ACHK3A_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO8741626.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(3924..5441)
                     /gene="lysS"
                     /locus_tag="ACHK3A_003589"
     CDS             complement(3924..5441)
                     /gene="lysS"
                     /locus_tag="ACHK3A_003589"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO8741627.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
     gene            complement(5678..7135)
                     /gene="dtpC"
                     /locus_tag="ACHK3A_003590"
     CDS             complement(5678..7135)
                     /gene="dtpC"
                     /locus_tag="ACHK3A_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO8741628.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7194..9341)
                     /gene="cadA"
                     /locus_tag="ACHK3A_003591"
     CDS             complement(7194..9341)
                     /gene="cadA"
                     /locus_tag="ACHK3A_003591"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO8741629.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSSWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYEDMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9421..10755)
                     /gene="cadB"
                     /locus_tag="ACHK3A_003592"
     CDS             complement(9421..10755)
                     /gene="cadB"
                     /locus_tag="ACHK3A_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO8741630.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTAGTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAGSGAPFAISASTILGNWAAPMVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(11121..12659)
                     /gene="cadC"
                     /locus_tag="ACHK3A_003593"
     CDS             complement(11121..12659)
                     /gene="cadC"
                     /locus_tag="ACHK3A_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO8741631.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(12919..13030)
                     /locus_tag="ACHK3A_003594"
                     /pseudo
     CDS             complement(12919..13030)
                     /locus_tag="ACHK3A_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(13470..14312)
                     /locus_tag="ACHK3A_003595"
     CDS             complement(13470..14312)
                     /locus_tag="ACHK3A_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8741632.1"
                     /translation="MSDITVSRPEVVNWHTDVICSTSILHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLEIMLHLFSGKPIPDNR
                     ENITIHLDDHVQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAI"
     gene            complement(14397..14594)
                     /locus_tag="ACHK3A_003596"
     CDS             complement(14397..14594)
                     /locus_tag="ACHK3A_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO8741633.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(14606..14845)
                     /locus_tag="ACHK3A_003597"
                     /pseudo
     CDS             complement(14606..14845)
                     /locus_tag="ACHK3A_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            14912..16074
                     /locus_tag="ACHK3A_003598"
     CDS             join(14912..15173,15173..16074)
                     /locus_tag="ACHK3A_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFO8741634.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            complement(16071..>16324)
                     /locus_tag="ACHK3A_003599"
     CDS             complement(16071..>16324)
                     /locus_tag="ACHK3A_003599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741635.1"
                     /translation="QEVFKMREISDNMLDSVKGGMNLNGLPGSTNVIDLRGKDMGTYI
                     DANGACWAPDTPSIIIYPGGSGPSYSMSSDPTHVIWTQA"
BASE COUNT         4415 a         3811 c         3836 g         4262 t
ORIGIN      
        1 tacagtaata attattactt acagataacc agcgatccgt tattggcaat attgtttcag
       61 cagtgagtag tgttctgcct gaatacggta acggtaaacc ggacgccctg tgacgccata
      121 atggatactg gtgaacaaga tgtggcagtt gaccagccag atgaggtatt tacggcagga
      181 aacacgcgag atgttaacct cgttggccag ctcgtcggtt gaaaactcat agtcctgatg
      241 cgcgtcaatc cactggcaca gcgtgcgtaa cgtctgcggc gtcaagcctt ttggcaagcg
      301 acgaggatcc tgttcgttgg agctgctgcc gtggattagc tgatcaagct cggcctggtc
      361 ataatactga tgtttttcca gcgccatttt cttttgccgc cagccggtga gcgcctcttc
      421 aaagcgggaa gcctggaagg gtttgatcag gtaatccacg acaccgtaat gcagcgaatc
      481 tttaatggtt gccgcatcgg ctgcggagga gatgacaatc acatcacttt tgcaacgcgc
      541 gttatgcagg acaggcagta aatcgagccc gttctctttt tgcatataga tatcgagcaa
      601 tatcaggtcg ataggcgtat cgctattgaa gataatctct ttggctttct ccagcgtcga
      661 agccgttcca cagcattgaa agcctgggat ttgtgccacg tatcggcgat tcagctccgc
      721 gaccattgcg tcgtcatcga taattaatac attgatcatc tgttcgacct ctccccgtcc
      781 cagggtatct ggacaaaaaa ttgtgtgaaa atcccgggtt ccgattccac ggcgatgctg
      841 ccgccgagat tttctacctg ttgtttgaca agtgctaaac cgacgcctcg ctcgcttcct
      901 tttgtcgaga cacctttgtc aaaaatgtga tcgattttgt cgggtgcgat ccccggtcca
      961 tcatcgttaa cttcacagtg cagccagccg tgacggtagt gcaatgttac gctaatttcg
     1021 ccaccgggtt ccggtcctaa tgcctccagc gcgttttcta tcagatttcc cagcgtggta
     1081 atcagcgtcg cgacctggtc ttcactgccg ctgtctggca actggctttc actgtttaaa
     1141 atcagcgtat ggcctaaatc ggtcgcgcgg ttaatcttac tgattaaaaa accagcgata
     1201 accggagatt tgatcttacc cagcagagag ccaatctctt cttgatagtt attggctgtt
     1261 ttgagaatgt aatcttccaa ctgcttataa ctcttcagat gcaataatcc gagaatcacg
     1321 tgcaatttat tcataaattc gtgggatcgt tcacgaagtg cgtcagcata gttgaccaga
     1381 ccatcgagtc gctgcatcag tttacgtact tcagttttgt ccctgaaggt tgaaatggca
     1441 ccgatgataa cgccattact gcgcaccgga acggtgttga tcagtaatag ccggtcttta
     1501 atcgtaatct cttcgtcgcg ccgcggggta ccgtcgcgta acacttccga gacatctacc
     1561 acctgtgacc acgagtggct tagcgtcgac agcttctcat cgtcctgcga cttacggtaa
     1621 ttcagcaatt cttgtgcggc atcgttgatc agcgtgactt caccgcgatc gtccacggca
     1681 acgacgcctt ctttgatgga ctgcaacata gcctggcgtt gctcaaacag cgtggagatt
     1741 tcgtagggtt ccaggccgaa aaggattttt ttcagtacct taaccagaat gcaggtgcca
     1801 atcagcccga ccagcatgcc aaataatacc gaccagatga tgctccagcg actgtcattg
     1861 atctgttggg tcacgcgact taactcaagg ccgatcgcca ccacgccaat ttgtttatga
     1921 ttttcatcgt agatgggggt aaatacgcgt aaagcctgcg ccagaaaacc gcgattgata
     1981 gcgacatttt cttcgccatt cagcgcttta aggatgtcat cacctttaaa tggctgacca
     2041 atacgctggg cttcaggatg cgagtagcga agactttgca tatcggtaac gacaataaac
     2101 agcagatcgt tgcgtttgcg cacggcttcc gcgatggcct ggattccact ctcctgtggt
     2161 tttttctgca agccctgacg gatttccggc gagtcggcga gggtacgcgc tactgccagt
     2221 gccttgttag ctagcccgtc tcgcgtcata tcactgattt gcgagaagta aatcagatgc
     2281 accaccaata gcaccgagaa cagtaccgcg ctgaccatta agatcactgt ggtactcaat
     2341 ttcatcggac gtttgcgtaa catgcggtag ggcaatgaat gtctcatcag cttccttgtg
     2401 tgacaaattt cttaagcatt atctctgatg aggcgggtaa ttcaaaggga gtaagaatga
     2461 ttggcatata ggggaagaga ctctggcaac ggaaactgcc agtgctgtat gaagattcca
     2521 gggctatgct tatagtgaaa accatactga tgagagaggg aaggtcatgg atcaggcgct
     2581 actggacagg ggttatcgct gttataccgg cgaaaagatc gatgtctatt tcaacactgc
     2641 gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta atctcaaacg
     2701 aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag tgattgatac
     2761 gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat ggaaatacgc
     2821 gaaggccaca ataaatttta cattaatgac gaacagggca agcaaatcgc tgaaattgtc
     2881 tttgtgccga ccggagagaa tttagcgatt atcgaacata ccgatgtcga tgaaagcctg
     2941 aaagggcaag ggattggtaa acagctggtt gcgaaagtcg tggaaaaaat gcgtcgggaa
     3001 aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa aacgcgggag
     3061 tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc atgctctccg
     3121 cggtgtgatg caggagagca tctgaagggt agggggatgc acaaagaatg ggcagagagc
     3181 gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat ctcctatatg
     3241 agaatcatca atcggggtta ataagttttg cgtccccaga gcgtttaata ttgataggag
     3301 tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg attatccatc
     3361 atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga gtttctcctc
     3421 tatcgtggct gatgaatatg ggataccacg acaactgaat gaaaactcct tcgcaataac
     3481 gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct tagacttacc
     3541 ggatattgac tttgacctca acattatgac agttgatgac tatttccgtc agttttacaa
     3601 gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa tattaatttt
     3661 ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac atgagaaaac
     3721 acatattcgt ttacttagtg catttctggt cggaataacc tggccaatga gtctgcctgt
     3781 ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg gttatcttga
     3841 aacgaagcgt ctctcctcaa aactggattt gcggaatgac gaacgaagca gtcgcctgac
     3901 tgcttcgttc attaaagtga aatttatttc agtgggcgca tcgccgggaa gagaataacg
     3961 tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc aatacccaga
     4021 ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc atagaacatg
     4081 gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc agcctgatct
     4141 tctgcgtcgt ttaattcgct aaaaccatta ccaatttcac gaccaccgat gaagaattca
     4201 aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga cacttctgcc
     4261 ggatattccg tgataaaggt tggctgaatc agatgtgctt ctgccacttc atcaaagatc
     4321 tccgtgacaa tgcgtcccaa cccccagctt ttctctaccg taattccgat agattcagct
     4381 aatgctttag cagcatcaaa attatccaga tccgccatat cggtttctgg acgatatttt
     4441 ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa cacatgctcg
     4501 ccataagtga ctttagtggt acccagtacc tcttgtgcca gagtgcggaa cagtgattcc
     4561 gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat catcgtgaac
     4621 tcaggattat gacgaacaga aataccttca ttacggaagt tacggttgat ttcgaatacc
     4681 cgttcaaaac cacctacaac cagacgtttc agatatagct ccggcgcgat acgcaggtac
     4741 atatctaaat ccagagcgtt atgatgggta ataaacgggc gagcagatgc tccacctgga
     4801 attacctgca tcatcggggt ttctacttcc ataaagccgc gagcgaccat gaactgacgg
     4861 atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc gttagcgatg
     4921 aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg gaatttatct
     4981 ggtaaaggac gtagtgcttt agtcagcagg cgcagctcag tacagtgaat ggaaagctca
     5041 cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag atcccatttt
     5101 ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta cagttgaata
     5161 cggccaccga catcctgcaa cgttacaaag gaggctttcc ccataatacg acgggtcatc
     5221 attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt atccttcgca
     5281 tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt gggaaacgcc
     5341 acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc atcgttaaaa
     5401 tcaatagcct cattggctcc ccgtgtttct cgttcagaca ttttggttcc tctaaatcca
     5461 gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct gcctcgacta
     5521 ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata tcactaataa
     5581 ataaccataa aaatggttta aatgacttat taatctcgat ttgctagtgt gataaactga
     5641 aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc tcctgtatca
     5701 tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caataccacg atgatcgcga
     5761 cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagcg gcaattgcca
     5821 taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc cagttggcga
     5881 ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc agacgcgtga
     5941 tttgcgcaat cgccaccggg tcaataaaga gttcagcaaa gcccattagc gccagcccgg
     6001 atatcatcac gcccattgac gcttgaccgc cagccgctgc atgtcgggca tcaaatgcca
     6061 gcaacataaa gccacaagcc atcagcagta ggccaaaggc aaacttcagc cagacgcgca
     6121 atgctgaatt gccgcggctt tctggcgacg ccagccaggc cagaacaacc ccagcgagca
     6181 tcaccgcaat agcattcacc gactggaata gtgctgtagg tacttcaata ttgaatgcct
     6241 gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt tgtgccagta
     6301 cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca cggcggtgtt
     6361 cggggaattt gatcatcatg cgggcaatga tttgtgctgc aatgaggcaa acgatcgcca
     6421 gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg gctaaacaga
     6481 gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg agcgcttttt
     6541 tatccatact acgcgtggac tggaaatgac gatgaccact taagaaaatc aacaaaccga
     6601 taaacatgcc gcctcccgca agggcaaagc caacatgcca tccataccac tgagcagcca
     6661 ggccgcaggc gatgggcgcg gcgatagaac cgatattgcc cgcagcatat agcagcgaaa
     6721 aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa cagctgatgt
     6781 ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac aggctaaagg
     6841 ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc gcgccggcaa
     6901 tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga ataggggtaa
     6961 cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca tcaaaaccaa
     7021 gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg aagtactccc
     7081 agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt ttcatgtgtt
     7141 ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc taattatttt
     7201 ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg gtatgcaccg
     7261 tgaatatcgg tttcaaagcc cggatagtga gcgccgattt cacacagcat ctgcaggaac
     7321 tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag aggaactccc
     7381 ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc gaggtaaact
     7441 tcttcggtca taccgtgcag ctctttctgg aacgccgcat acggagtcat aaccattgtc
     7501 ggcagaactt caaatgcgcg atacatcaga tccggcagat tgtggtgaac aatcagtttg
     7561 tggatattct gagccagttc ctgaatacgc atatcttcat agaattcagg atcttcacga
     7621 tatagagacg gcagcatgtt tttcacacgc aggttcaggt cgaacgcacg tttgaagtca
     7681 gtcagagcac gcagcaggct caatgctttg gtcttatcaa taccgatgct gaacaggaac
     7741 agcaggttat acgggccggt tttctcaaca acgatgccat gttcgtcgag gtatttcgcc
     7801 acgatgctgg ccggaatacc aaagtcgctc atggtgccgt ctttttccat ccccggggtc
     7861 agcagggtga ctttgatcgg gtcaagatac atgtgctcat tgtcgatgtt cttgaagccg
     7921 tgccaggagc tgtcagaacg cagcggccag cattcagtgg tatcgatatg atccggctgc
     7981 catacatcaa agaaccagcc atcagattcc gttctcagac gtttgatctc tttacggaac
     8041 ttgatcgcac gttcaatgga accgttgatc agacgcttac cagcattacc tttcatcatc
     8101 gccgcagcgg tttcagtgga cgccacgata ccgtagtgcg gagaggtggt ggtgtgcatc
     8161 atgtaggctt cgttaaaggt ttcttcgttt acgtcacctt taacgtggat catggaagcc
     8221 tgagagaacg ccgccagcag tttgtgagtg gactgggttt cgtaaatcac tttcccttct
     8281 acacggccac cgctcatacc gcatttacct tcgtaaatcg gcgagaagtt ggtgtaaggc
     8341 acccacgcgg agtcaaagtg gatggatttc acatccagcg ttttcttgat gaagtcggtg
     8401 ttgtacagca gaccatcata ggtagagttg gtaattacgg catgtaccgg ccaggttgcg
     8461 tttggtgttt ctttaacgcg cttagcaatg gttgcgtgct ggaattcact ctgtgggata
     8521 ccaccaagaa taccgtaagc gttacgggtc gggcggaaat agattggggt aacatcgctc
     8581 atcatcatta ggtgggttag cgatttgtgg cagttacggt caatcagaat ggtgctgcct
     8641 gccggagcag agtacatacc gacaattttg ttcgcagtgg aagtaccgtt ggtcaccatg
     8701 tagctgcggt ctgcgttaaa gacgcgagcg atatactgtt ccgcttcttt gtgtggaccg
     8761 ctgtgatcca gcagagaacc caattcagat actgaaatgg aaatatcaga tttcatggtg
     8821 ttcggaccaa agaaatcata gaacaggcta cctaccgggc ttttctggaa tgcagtaccg
     8881 cccatgtgac caggagtaca gaaagtatat ttaccttcac gaacatattt aaacagtgct
     8941 ttggtcagcg gaggcagaat agtgttgata tattcgtcag tggtctgctt gatcttgtta
     9001 gcaatatcat cagcagcacc cagcgcatat tcaaagaagc taatctgtaa acgcagatcg
     9061 ttcaggctta catcgagtgt ggaatacgta ttagcgaacg cgtacaacgg caggttctcg
     9121 ttcattttgc taatttcttc gcacagctcg agattatatt tatcccagtc aaaaataaca
     9181 ccgcacagac gcgcattgtt ttcgatcagt tttaataagt cgtcacggtc attcgggtaa
     9241 acaatctgga agttcagacg ttcaagcgcg cgatgaagtt cacggatggg ttcttcttta
     9301 aaataaacac ccatgtgatt caatattgca ataacgttca tagtcatatc tccaggtaaa
     9361 aaaggcccct cccaacacat gggacaaaat gaaaggagga gcctcggaaa atacttttaa
     9421 ttaatgtgcg ttagatgctg tgtggttatc cattgagtgg ctctggcgct cgtgcatttt
     9481 gcgagcgtag aacatcagga taatcaggct gacgatgaag gtaccagcca gctcgaagga
     9541 gcttgcgccc atcagagcga tgaagcagaa cacgcaaccc agtacagagc agatcaggct
     9601 gacaaagttg cggatgttaa cgccttcaaa acgaatcagg tcaacgcaag agtagaagta
     9661 cggcagcata gtcagcagta ctgcgatacc ggtcagttca ccgaacaggt cagatgcttt
     9721 accaccggca gagttcatca gagtgatgag gatcatcagg gcagtcattt tcactgcagc
     9781 cagcagcaga ccttttttcg gaataccgtt gctgtcgact tcaccataaa ctttcgggaa
     9841 gttaccgtcg ttagcggcac gcacacctgc ctggccgacc agcatcatcc aggagcccag
     9901 agaagtcagg cacgcaaaag cggtgaatgc agaaaccatc ggcgcagccc agttacccag
     9961 gatagtagaa gcactgattg caaacggagc accggagcct gccattacag aagacggata
    10021 cataccggaa agaacctgag tcgcagcaat gtaaacaata cctgctaaac cagtccccag
    10081 catggttgcc agtggaacag tacgtttcgg gtttttaacc ataccagtac ttacagctgc
    10141 ggattccaca cccacgaagg cccacaggca gagcagaata cttttaatga ttgcatgacc
    10201 atcagtagta cctgcggtat tccagttagc tgcataagtt gcagcatcaa accaatgcca
    10261 gccaacaata gcagtcataa ccacaggaat aagaaccagt accaggccaa tagtggttaa
    10321 acggcttacc caggtaccgc cgagcatatt tacaaaggta aatacccaga cgatagcaat
    10381 acaggcgata cccgccggaa caggatcatt taatactggg aagaaggtgg aaagataaga
    10441 tacagcggta ataccaattg ccaggttacc aatccagtta gcatggtaat aaagaacacc
    10501 tgtctgaaaa ccaaatgcag gagaaatttc tccggcatac gcaattgggc caccttgttg
    10561 cgggtttttt gttgccagtc gggcatatac atatgccagc gacattgcac caataataga
    10621 gataatccaa ccccagatag caataccacc gatacttgct aggttcgcag gtaataatgc
    10681 aataccgctc cccatcatat taccggcaac aacaccggta caggcaaata gcccgatctt
    10741 cttggcagaa ctcatgctct tctcctaatt tcatttttga atttggagtc cgggtcatga
    10801 tgtataacta tttcctgacc agaccaaact ggcgctaaga ttactcacga aaaaaggatt
    10861 aatcctaaag attaggtgaa ataacacaaa agtttctgta agtgagaact tgaggttttt
    10921 tattaacaca tcaggatcgc aagttgatat catgaaaaga taaacattca atgtttacaa
    10981 tgtgttatgt gacattttct ggttaaattt atgtaataaa aattatgcgg caaataaatt
    11041 gccgcaacat attataccaa caggaacata caaaaactca acaacaaata ttcccgagca
    11101 tgaatcaacc cgggagttac ttattctgaa gcgagaaatt tgtcgagata aggtacaaca
    11161 taaggaacag aagtctggaa tataccattt tcaatccagt aaagggtgtt tgcccctggg
    11221 cgtaaattaa aggcggtgag atatgcatca gctgcttccc ggttcatccc cttcatttca
    11281 taaaccttgc caagcaacac ataatttagc caggacattt caagatcaat gccagtattt
    11341 atcgcctgat aagactcatc tgttttacct tttaccaggg cactgaccgc ttttatttga
    11401 tatataatgg acaggttgtt caattccggc agtgtaacaa tgttatctat ttctgtgtta
    11461 agtgctgcta attgtttttc gtctaaagga tgttgagaat ggcgcacgat atcaactaat
    11521 gctttttctg ctctcgcgta ggtaaattct ggggatgatt gaacaatctc acctaataat
    11581 tcactggcac gatccaatga tttatcatca ccatgcagta aataatcatg tgcctgataa
    11641 aaattagtta ataacgcacc acgatgcggc aaaatttgct ggagcatctc ctgcattcgt
    11701 tgtggccacg gttggtttaa cgcttttgat aaactctcca gtaaatcatt ttgaatcgcc
    11761 agctgattac cgttagtgat gacataacgt ttatccagca tggttgaacc atctgcattg
    11821 tctaccaatt ttaccgacat aaagcattgt tgagcacggt attggcgctg attaacaaac
    11881 gcaatagata atgttttacc ggaactgctc ggttcatcaa tgttgtagtt gattttgtca
    11941 tgcaccataa aggtggagaa ggtgttaagt gatgtcgcca ccaaatcacc cacgcctatc
    12001 gcgtaagaga gctgatacgg ggaactccag ctgttacaac ttttatttac catattaatg
    12061 tcaatatcgc gtggattgag caaaatacgc gatttgctca tcggaagacg tgtatcaaga
    12121 cttgaaaacg ctaccagtgc tacacagata cctaacgaca acaggaaaaa aaaccatacc
    12181 caaaaggtag tgaatcgttt gcttttaact ggggattgtt caggtggcgt tgcggtgttt
    12241 tgaatgttaa gactgtggga gggagaatct gtggcaggaa ccgcctctgg tataggggga
    12301 ggcgaagata gcattatttc ctctccctct tcttcgctgt accagataac cggcaccatt
    12361 aatttatagc cgcgctttgg tacagtagcg atatagacag gactatcttc atcattatct
    12421 tttaatgact tacgtagttc tgagatactc tgcgtcacaa cgtgattggt gacaatactt
    12481 ctcttccaga cattatcgat aagttcatcc ctgctaagta cttcgccact gtgttgagca
    12541 aagaaaacca gaaggtcgat taatctcggc tcaagggtaa gttgacgccc attgcggcta
    12601 atttggttta tggacggagt aacaagccat tcgccaacgc gaactacagg ttgttgcata
    12661 atagaaactc attcgaaaag ggaatggtgc aatgataatt ggcataacct atttttacca
    12721 tctatagatg ggtttattta catattattg gtgaatgcaa gacgttattt ttaccagaca
    12781 taaacttctt gattacatag tgttgagaaa ggattttact gagaaccaga agtaatattc
    12841 cttaccatca aaattcatca tctttgccaa agaaaaatgt tcagaaaata atccatggaa
    12901 aattgtccgg agcgcttact attttaatgg attgttagtc tttgcatgag caaacggact
    12961 gatacatttc tctttgttct cattcagaaa atctcatcag ttggcgttct gcccgatgtt
    13021 gtgctttatg ggtgcgttac gctcctgatg acgtcatttg acgttcaaca gcatcacggg
    13081 gccgcacgac atttcacgtc agtcagcgct atagctcaga aacaaatttt cccgaattgg
    13141 aatatgcccg caaattgctg gggatgtggg gagaatctgg ttgagttcgg tagaattgat
    13201 ttggcggcag gacgcttaaa tcgtggcgtc ctgaaacgaa aaacggacct ccgtggaggt
    13261 ccgtttatat gaatttggtg ccggactcgg aatcgaagat ttcatttatc atattgatat
    13321 taatctcgtt tatttggttt gatgaaaaat gtaccctctc ttgtaccctc ggtcccttct
    13381 ggtggttttt atgagaacaa gtagatcaag atttttgtca tcagaacacg tgtatttttg
    13441 tgggttttat tcctgagttt tattcctttt tatatcgcta acgctccggg atagtgctta
    13501 gcaataatat cattaagcct gtcaaccagc tctggtttcc tgaacgtgat gtgcgcggag
    13561 cccttcttaa agtatctgat gctgaacatc tcatcttcat agtcctcttt accctgaaca
    13621 gactggacgt gatcatccag atgaatagtg atgttttccc ggttgtcggg gatcggtttg
    13681 ccgctgaaca ggtgtagcat tatttccaga tcagcaagtc gatctgcctg ttgcccggtg
    13741 ataagatgaa agccccaccg gtcccacctc accaggttgt tgacgataat tttactgcca
    13801 aatttgcagg ggcaattggt tttgtaattc cagctcaacc ctctgaagac gttgatcacc
    13861 ccccgctcaa acacctcatc cttattctgg tgcagttgtt caaatgtgct cagaatgttc
    13921 gcttcactga tttccggaaa attatcgtat tccagtgacc ggtaccatgt atcacgggcc
    13981 tgtgcatcca ttaaggaaag catcccggaa cgttgcatca ggtcacgcca gatttcgcga
    14041 tcgagattgc gtgtgatggc tttcattgcg gtttcaggtt tctccatcag ccagcaaccg
    14101 cagtgaaaat tctgtttcat ggcccagtcc agtgcggttt taccgccaat gctttctgtc
    14161 attgctgaga tttcagccag ttgccggata agtgtgtcga tttgcagcag tgtactcttt
    14221 cgtacagcca gaatgtggag gatactggtt gagcagataa cgtccgtatg ccagttgaca
    14281 acttccggac gggagactgt gatatcagac ataatcgata ttcctttttg cagataaatg
    14341 aaaacgccag ccctgttcag gctggcgtca gtggaatgaa gtctggtgag tgagagtcac
    14401 cggttctggt gcagaagctg caactggcgc agtgtacgga tatcttccct ggcctgttca
    14461 atgcagggca gcagtgctgc cgaatccgtt ttatcctcat cgttgtcaaa gataattccc
    14521 gattcgcagt cgatattgtc ctgcagccac gcaatcagaa tatccagcgc tgtttccgtg
    14581 gttaatgatt tcatgttgtt tctggttacc agtcgaaagt gggtaaaccc ggcaggcatt
    14641 cagcgctggc atccagatga atgagactga cgccataacg tcgggtaagt gtgacaacca
    14701 gacggcggaa cgtttttgac agccccaggc gtttaagccg cagaaccggg tatgaccacg
    14761 catcggtacg taacagatat ccgctacctg tgaaatgaat ccattctgat tcgccggtgt
    14821 cattcgtctg atgtgacagc gagtactgat cttacccagc aatagtggac acgcggctaa
    14881 gtgagtaaac tctcagtcag aggtgactca catgacaaaa acagtatcaa ccagtaaaaa
    14941 accccgtaaa cagcattcgc ctgaatttcg cagtgaagcc ctgaagcttg ctgaacgcat
    15001 cggtgttact gccgcagccc gtgaactcag cctgtatgaa tcacagctct acaactggcg
    15061 cagtaaacag caaaatcagc agacgtcttc tgaacgtgaa ctggagatgt ctaccgagat
    15121 tgcacgtctc aaacgccagc tggcagaacg ggatgaagag ctggctatcc tccaaaaggc
    15181 cgcgacatac ttcgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcaggct
    15241 gagttcagca tcaaagcaat gtgccgcgtg ctccgggtgg cccgcagcgg ctggtatacg
    15301 tggtgtcagc ggcggacaag gataagcacg cgtcagcagt tccgccaaca ctgcgacagc
    15361 gttgtcctcg cggcttttac ccggtcaaaa cagcgttacg gtgccccacg cctgacggat
    15421 gaactgcgtg ctcagggtta cccctttaac gtaaaaaccg tggcggcaag cctgcgccgt
    15481 cagggactga gggcaaaggc ctcccggaag ttcagcccgg tcagctaccg cgcacacggc
    15541 ctgcctgtgt cagaaaatct gttggagcag gatttttacg ccagtggccc gaaccagaag
    15601 tgggcaggag acatcacgta cttacgtaca gatgaaggct ggctgtatct ggcagtggtc
    15661 attgacctgt ggtcacgtgc cgttattggc tggtcaatgt cgccacgcat gacggcgcaa
    15721 ctggcctgcg atgccctgca gatggcgctg tggcggcgta agaggccccg gaacgttatc
    15781 gttcacacgg accgtggagg ccagtactgt tcagcagatt atcaggcgca actgaagcgg
    15841 cataatctgc gtggaagtat gagcgcaaaa ggttgctgct acgataatgc ctgcgtggaa
    15901 agcttctttc attcgctgaa agtggaatgt atccatggag aacactttat cagccgggaa
    15961 ataatgcggg caacggtgtt taattatatc gaatgtgatt acaatcggtg gcggcggcac
    16021 agttggtgtg gcggcctcag tccggaacaa tttgaaaaca agaacctcgc ttaggcctgt
    16081 gtccatatta cgtgggtagg atcactactc atactataag agggtccact tccccccgga
    16141 tatatgatga tggatggagt atccggagcc cagcatgctc cattagcatc aatatatgtt
    16201 cccatatctt ttccacgtag atctattaca ttagtagaac caggtaatcc attaagattc
    16261 atccctcctt tcacggaatc aagcatgtta tctgatattt ctctcatttt aaaaacctcc
    16321 tgct
//
LOCUS       DAZGHF010000107        15954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGHF010000107 DAZGHF010000000
VERSION     DAZGHF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.107"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(7..369)
                     /locus_tag="ACHK3A_003600"
     CDS             complement(7..369)
                     /locus_tag="ACHK3A_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741636.1"
                     /translation="MKKYILVSLMGLSLNAFAGGSGWNPDRVDPSRCVMATGSGPTSA
                     GYMVYRTTDSVCMQGINEGKVKGVHIYDGHHEFNDGTVDTFSGNVTPISPLTLKTDVQ
                     KVNKVGIKRWWYKASWIK"
     gene            920..1365
                     /locus_tag="ACHK3A_003601"
                     /pseudo
     CDS             920..1365
                     /locus_tag="ACHK3A_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
     gene            1409..1677
                     /locus_tag="ACHK3A_003602"
                     /pseudo
     CDS             1409..1677
                     /locus_tag="ACHK3A_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1882..3000
                     /gene="ybdK"
                     /locus_tag="ACHK3A_003603"
     CDS             1882..3000
                     /gene="ybdK"
                     /locus_tag="ACHK3A_003603"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO8741637.1"
                     /translation="MPIPDFQVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLMEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            3066..3314
                     /gene="ybdJ"
                     /locus_tag="ACHK3A_003604"
     CDS             3066..3314
                     /gene="ybdJ"
                     /locus_tag="ACHK3A_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO8741638.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            3379..3747
                     /gene="ybdF"
                     /locus_tag="ACHK3A_003605"
     CDS             3379..3747
                     /gene="ybdF"
                     /locus_tag="ACHK3A_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO8741639.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            3841..4494
                     /gene="nfsB"
                     /locus_tag="ACHK3A_003606"
     CDS             3841..4494
                     /gene="nfsB"
                     /locus_tag="ACHK3A_003606"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO8741640.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQVKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLEEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            4602..5849
                     /gene="ybdG"
                     /locus_tag="ACHK3A_003607"
     CDS             4602..5849
                     /gene="ybdG"
                     /locus_tag="ACHK3A_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO8741641.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(5930..7306)
                     /gene="pheP"
                     /locus_tag="ACHK3A_003608"
     CDS             complement(5930..7306)
                     /gene="pheP"
                     /locus_tag="ACHK3A_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO8741642.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(7408..10551)
                     /gene="cusA"
                     /locus_tag="ACHK3A_003609"
     CDS             complement(7408..10551)
                     /gene="cusA"
                     /locus_tag="ACHK3A_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO8741643.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGQTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(10563..11786)
                     /gene="cusB"
                     /locus_tag="ACHK3A_003610"
     CDS             complement(10563..11786)
                     /gene="cusB"
                     /locus_tag="ACHK3A_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO8741644.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWITAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADESLKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(11802..12134)
                     /gene="cusF"
                     /locus_tag="ACHK3A_003611"
     CDS             complement(11802..12134)
                     /gene="cusF"
                     /locus_tag="ACHK3A_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO8741645.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KTIDLESKKIIIHHDPITAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(12158..13540)
                     /gene="cusC"
                     /locus_tag="ACHK3A_003612"
     CDS             complement(12158..13540)
                     /gene="cusC"
                     /locus_tag="ACHK3A_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO8741646.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLNQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSNNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            13697..14380
                     /gene="cusR"
                     /locus_tag="ACHK3A_003613"
     CDS             13697..14380
                     /gene="cusR"
                     /locus_tag="ACHK3A_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO8741647.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            14370..15818
                     /gene="cusS"
                     /locus_tag="ACHK3A_003614"
     CDS             14370..15818
                     /gene="cusS"
                     /locus_tag="ACHK3A_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO8741648.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQAQRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDTRGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFMIKLPEKG"
BASE COUNT         3916 a         4240 c         3843 g         3955 t
ORIGIN      
        1 tattttttat ttaatccagc tagctttata ccaccagcgt ttgatgccaa ctttattaac
       61 tttttgaaca tcagttttta atgtcaaagg tgatattggg gtaacattac cgctaaacgt
      121 gtcaacagta ccatcattga actcatgatg cccatcataa atatggacac ctttcacttt
      181 tccttcattt ataccctgca tacacactga gtcagtcgtt cggtatacca tataacccgc
      241 agaggtagga cctgaccccg tcgccataac acaacggctc gggtctaccc tgtctgggtt
      301 ccagccgctg ccacccgcaa atgcgttcaa acttaatccc atcagagata ctaaaatata
      361 tttcttcatt ataacctctt attgttaata gataatatat ttgtcatgca acaagttgca
      421 tgtactgcac ttacgctccc tcagtcactt acttatgcaa gctcttggga actcacttat
      481 acactacctg taactcgaat tatttagagt ataaaaccat tagcatatct agtatctaat
      541 agtgttttat tttattacat aatagaaaaa atctattatg tgcaattatt taatagaata
      601 ataacacgtg caacgaattt taagtgtttc ttgagtgaca taaaataagt gtggtgtcca
      661 tatcctccta tcgtgaatca atataaatga tttacaccta acactataaa atgttcagaa
      721 agccatcgca ataataaaaa ataagacatt acgataatat tataaagcat tgctttgttg
      781 ggggatgacg tatgtagcaa tgaaagataa caaatgccgg taatggtacg gagaaatcct
      841 cagtaatagg cgggtaaaaa agacgggcat agagttttgt ggtcgactgg ttgtgctaac
      901 aattcaggag atcacctcta tgcccgttcg ctcatcatgc caaaagtttt tccgtgatga
      961 tttgactcca tttcatcagt accgccagaa tgccatcctt gacgccactg ctgcgcttag
     1021 tcgggacgca tccctcatcc ttaccagcat tggacggtat atgccaggca atgctcaggt
     1081 taaaacaaga ttaagcccat cgatcgcctg ccaggcaatg acgctttaca ccgcgatatc
     1141 ccggctattt ttcataatat tacccggttt cagccccgag accttacgtt gtgcgtcatt
     1201 gccgttgact ggagtggtta tccttctcag gcattccatg ttctgcatac tccccaagtt
     1261 tgcgacgggc gttctatatt acttctggat caggttgtat cgtcattctg gcaacaaatt
     1321 gctcagttct ataagaattt tcttgatacg cttgcaatca ctattgctcc gaataaaccg
     1381 gataaaaatt acctttaaac acagcaccat gaccgccaca agggcatatt tcgtcagcat
     1441 gtttatattg cctccatgca aagaagggac taccatccaa attgtttggg tttggagtgg
     1501 cagccccagg attgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa
     1561 cctgaaaccg gaaagcttca ggcacccgcc gttatcttac ccttccttag caaaatggct
     1621 gtttatttat acagtaagtc ctgtttcgga cttctcaatc ctcatttgat caactgaagc
     1681 tgaacccgtt ttattctgca tgcctcaatc gtcaaaattc ttcgccacca gtccgcactg
     1741 ccataacttg tcatattgca aaatgtagcc aatttgcttc agtcaccatc atcaaaaaaa
     1801 gaaacattcc agaacttccg catctcgccc agccgctccg acttctatac tgaatagaaa
     1861 acgccaacat aagagaaacc tatgccaata cccgattttc aagtctctga accttttacc
     1921 ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt aagccaggac
     1981 tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg ccggagaggt aaagcacgat
     2041 atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc
     2101 gggcaatttt cagcgatgca gaaagtcgta ttgcaggcag ccgcagacca tcatctggaa
     2161 atttgcggcg gtggcacgca cccgtttcag aaatggcagc gtcaggaggt atgcgataac
     2221 gaacgctatc aacgaacgct ggaaaacttt ggctatctca tccagcaggc gaccgttttt
     2281 ggtcagcatg tccatgttgg ctgcgccagt ggcgatgatg ccatttattt gctgcacggc
     2341 ctgtcacggt ttgtgccgca ctttatcgcc ctttccgccg cgtcgccata tatgcaagga
     2401 acggatacgc gttttgcctc ctcacgaccg aatatttttt ccgcctttcc cgataatggc
     2461 ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc tgtttcgctg cttgagttac
     2521 accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag tcctcatttt
     2581 ggcacggtgg aggttcgggt aatggatacc ccgttaaccc ttagccatgc ggtaaatatg
     2641 gcgggattaa ttcaggctac tgcccactgg ttactgacag aacgcccgtt caaacatcag
     2701 gagaaagatt acctgctgta taaattcaac cgtttccagg cctgtcgcta tgggctggaa
     2761 ggcgtcatta ccgatccgca tactggcgat cgtcggtcac taatggaaga caccttgcgt
     2821 ttactggaaa aaatcgcccc ttctgcacat aaaattggcg catcgagcgc aatagaagca
     2881 ctgcaccgcc aggtcgtcag cggtctgaat gaagcgcagc tgatgcgcga tttcgtcgcc
     2941 gatggcggct cgctgattgg gctggtgaaa aagcattgtg agatctgggc tggtgactaa
     3001 gccgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
     3061 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
     3121 tcctttcttg cctgctgctg cccgcccccg cactggggct gacgctggca caaaaactgg
     3181 tgaccatgtt ccatctgatg gatcttagcc agctttacac tttattgttt tgtctgtggt
     3241 ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct
     3301 cgctggcgga ttaaacgtcg attatcgccg tacttcgcgg catactttgc ttattctcct
     3361 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctggcc
     3421 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
     3481 attggcggca agatttttat gctgtcgtcg gagctgcgcg gcgtcccctt tatcaatctg
     3541 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat acccgagcat caagccaggg
     3601 tatcacatga ataaaaagca ctggatttca gtgtatcccg gcgaggaaat ctccgaagcg
     3661 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
     3721 gatcaaaaaa gagtgcgtcc aggctaaagc ggaaatctat agcgcatttt tctcgcttac
     3781 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tactttcaca tggagtcttt
     3841 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagcaaa
     3901 aaactaaccc cggaacaggc cgagcaggtc aaaactctcc tgcaatacag cccatccagt
     3961 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     4021 gccaaatccg ctgccggtaa ttacgtgttc aacgagcgta aaatgcttga tgcctcgcac
     4081 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     4141 caggaagatg ccgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     4201 cgcaagttct tcgccgatat gcaccgtaaa gatctgcacg atgatgcaga gtggatggca
     4261 aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggcgct gggtctggac
     4321 gcggtgccca tcgaaggttt tgacgccgcc atcctcgatg aagaatttgg tctggaagag
     4381 aaaggctaca ccagtctggt ggttgttccg gtgggtcatc acagcgttga agattttaac
     4441 gctacgctgc cgaaatctcg tctgccgcaa aacattacct taaccgaagt gtaattctcc
     4501 cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt
     4561 tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc
     4621 aggttgaaga tttagcgggt attgagatcg atcacaccac ctcgatggtg atgattttcg
     4681 gtattatttt tctgaccgcc gtcgtggtgc atattttttt gcactgggtg gtactgcgga
     4741 ccttcgaaaa acgcgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata
     4801 aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat
     4861 tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga
     4921 tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgtgattttg aatctggcgc
     4981 agaaattccc ggcggcatct cagttaccgc tgaaagggat atttcagggg attaaactga
     5041 tcggcgcgat tctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga
     5101 ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggtattt aaagatccga
     5161 ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgact
     5221 ggctggagat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg
     5281 tcaaagtacg taactgggac aataccatta ccactattcc cacctggtct ctggtttctg
     5341 actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt aagcgcagta
     5401 tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata
     5461 aagcgcattt gttaaagcct tatttaacca gccgccatca ggaaattaat gagtggaatc
     5521 gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat attggaacct
     5581 ttcgtgccta tctgaacgaa tatctacgta accatccgcg gattcgtaaa gatatgacct
     5641 taatggtacg ccaactggct cctggcgata acggtttacc gctcgagatc tatgcgttta
     5701 ccaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gaccacatat
     5761 ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaactggc aatgatattc
     5821 gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg aatggatgag
     5881 agcggtttcg gatggttgac atcattttgt cggatacggc gcgaatgcct tatttccgac
     5941 gcagcgtttt aaacgctaca aacaggaata caatccacac tggcagcaga atcgctgaca
     6001 agcgcatatc atccatcgtg cacatcagca gcaaaatcat gccgaggaag gcgatgcaaa
     6061 gatagttgcc gaacggatag agcagcgcct taaactgtgt ttcacgtccc tgacgtcgca
     6121 tcgccgcgcg aaaacgcaga tgcgccaggc agatcataat ccagttcaac agcagcgttg
     6181 ctaccaccag cgccatcagc agaccaaacg ctttttgcgg cagcagatag ttaatcagca
     6241 ccaccagcga agtgatggct ccggaaagca tcagcgagtt aatcggcacg ccgcgacggc
     6301 tgacgcgagt caaaaacttc ggtgcattac cctgcacaga aaggccaaac agcatgcggc
     6361 tgttagagta aaccccgctg ttatacaccg acagcgatgc caccagaatg acgaagttca
     6421 gcgcagaagc taccacgttg ctgtcgagat tatggaaaat catcacaaac gggctactgt
     6481 tggatttcac ttccacccac ggatagagtg ccagtaaaac caccagtgaa ccgatataaa
     6541 acagcagaat gcgatacacc acctgattca ctgctttcgg aatgcttttt tccggatcgc
     6601 gcgcttcagc ggcagtaatc ccaatcagct ccagaccgcc gaaagagaac ataattaccg
     6661 ccagcgacaa aatcagccca ttccagccgg tggcaaaaaa accaccgtaa cgccagaggt
     6721 tatcgatact ggctttctcg ccgccgtgac cagaaaacag cagccacagg ccaaagccga
     6781 tcataccgat gatcgccagc actttaatca gcgcaaacca gaattcggtt tcgccatata
     6841 agcgcacgtt caccaggtta acggcgttga tgataataaa gaaggcggca gcccaaatcc
     6901 acgttggaac atccgggaac cagtactgca tatagatgcc cgcagcggtc agctctgcca
     6961 ttcccaccag cacgaacatt acccagtagt tccagccaga gaggaagccc gcaaacggtc
     7021 cccagtattt ataggcaaag tgggcaaatg aaccggatac cggctcctca accaccattt
     7081 cgccaagctg gcgcataatc aggaaagcga tgatcccggc gacgccgtag cccagcaata
     7141 cagccggacc cgccatctga atcgccggac caatgccaag aaacagacca gtaccaattg
     7201 cgccacccag cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga agcgtcggct
     7261 cttgattcga cgcagtatct tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg
     7321 tgtctttttt gttgaggggc accttttaac atttagtgcc catcgtcgca agacacaatc
     7381 cacacggtta aacggggtat cctgctttta tttccgtacc cgatgtcggt gcagccacat
     7441 cagcttatac gccgccggga taataaacag cgacagcaaa ggtgcggtga tcatgccgcc
     7501 aatcatcggt gcggcaatcc ggctcatcac ctctgaacca gcccccgttc cccacagaat
     7561 cggcagcaga ccggcgataa tcactgccac cgtcatcgct ttcgggcgca cgcgcaggac
     7621 cgcgccgtga tacaacgcct catccagctt ctgctcgctg aatgtttgcg gattattcaa
     7681 cgacggctcg gcctctatgg cgtgacgtaa atacatcagc atcaccacgc caaattcggc
     7741 ggcaaccccg gcaagggcaa taaaaccggt gcccgtcgcc acggaaagat gaaagcccat
     7801 ccaccacagc aaccagatgc cgcccaccag cgcaaacggt acgctgctga taatcagcaa
     7861 cgcttcgccc acccgacgga acgccagata taacagcacg aagatgatca tcagcgtcat
     7921 cggcaccatg agtttcagct tatggttggc gcgctccagt agctcgaact gcccggagaa
     7981 tgccacgctg gtgcccggtt ttaactgcac tttttcagct atcgcttttt gcagatcgtg
     8041 aaccaccgac accatgtcac gatcgcgggc atcgatataa atccagctcg tcgggcgcgc
     8101 attctcggtt ttcagcatcg acggtccggt agagacttta acgtcagcca cgtctgccag
     8161 ggtgatttgc tgcttcatcg gcgtgaggat cggcaactgg cgcagtgcct gcgggctatc
     8221 gcgccagctt tgcggataac gcagattaat tggataacga gcaatccctt ccaccgtttc
     8281 gccaaccatc gccccgccca ccgcagaagt cacaaacaac tgcacatccg ccaccgtcat
     8341 accgtaacgc gcggcttttt cacggttaat ctcaacgttg atataacgcc caccttccag
     8401 acgctcagca agcgcagaag ccacgcctgg caccgttcgc gccacttctt caatttgctc
     8461 cgccatcgcg tcgatatccg ccagcacggt gccggaaact ttaataccga tgggactttt
     8521 aatgccagtc gagagcatat cgatacggtt acgaattggc ggcacccaca gattcgccag
     8581 ccccggcaaa cgcacggtgt tatccagttc ctcaatgatt ttgtccatcg tcatgccagg
     8641 ccgccactga tcctgcggct taagctgaat ggtcgtttct accatctcca gcggagcaga
     8701 atcggtggcg gtttccgctt tcccggtttg gccaaatacc cgcgccactt ccggtacgct
     8761 cataatcagc ttgtcggttt tttgcagcat actcgccgcc tccgctgcgg aaatccccgg
     8821 cagcgtcgat ggcatataca acaagtcgcc ttcattgatc tgcggtaaaa attccccgcc
     8881 aactttattg agcggccaga gaaccgtcag caccgaaagc gctgccacca acagcgtggt
     8941 tttcggccag tgcagtactt tcagcaacag cggatgataa acacgaatca aaaagcgatt
     9001 gagcgggtta ctgctttccg gcggaatttt gccacggatc cagtagccca tcaggatcgg
     9061 gatcactacg atcgccagta gcgccgcacc cgccatcgca taggttttgg tgaacgccag
     9121 cgggccaaac agacgccctt cctgcccttc cagggtgaag atcgggataa acgacagcgt
     9181 gataatcagc agactgataa acagcgccgg ccccacttca acagatgcat cggtaatcac
     9241 ctgccagcgc gttttattat ccaacgtggc gtcaggatgc tggtgctgcc actcttccag
     9301 ccgtttatgc gcattctcga tcatgacgat agcggcatcg accatcgccc cgacggcaat
     9361 cgcaatgcca cccaacgaca taatattggc gttcagtccc tgaaagtgca tgacaataaa
     9421 agcaatacac aaccccagcg gcaacgaaat aatcgccacc agcgccgagc gcacatgcca
     9481 gagaaacagc gcacagacta ccgccaccac aataaactct tccagcaact tgccgctgag
     9541 gttgtcgatg gcgcgatcaa tgagctggct gcgatcgtat gttgtaacta tctccacgcc
     9601 ttccggcaga ctacttttca gcgtttccag tttgtccttc acggcggcga ttacttctcg
     9661 ggcgtttttg ccggagcgca ggatcaccac cccgcccgcc acttcgcctt cgccgttcag
     9721 ttcggcaatg ccccggcgca tctccgggcc aatctggacc ttcgcaacat cacgcagata
     9781 aacgggcacg ccattttcac tggcttttaa aacgatgtga ttaaagtcgt cgagcgtttg
     9841 cagatagccg ctggctcgca ccatatattc cgcttccgcc agttcgatcg acgaaccacc
     9901 cgcttcctgg tttgaagcat ccagcgcgct ttttacttcg gcgagactga tgccatattg
     9961 cgccaggcgc tgaggatcga taaccacctg atactctttc accacaccgc ctaccgacgc
    10021 cacttccgca acgtcaggaa tggttttcag ctcatatttg agaaaccagt cctgcaatga
    10081 gcgtaaatcg gcaagatcgt gcttaccgct gcgatccacc agtgcatatt catagatcca
    10141 gccaacaccc gtggcatctg gccccagctc ggcgctgact cccgcaggca gcttaccctg
    10201 tacctggttg aggtactcca gcacccgcga gcgcgcccag tacggatcgg tgccatcttc
    10261 gaaaatgaca tacacataag agtcgccaaa ctgcgagaaa ccgcgcacag tcttcgcgcc
    10321 aggcaccgac aacatggtgg tggttagcgg ataagtcacc tgattttcaa cgatttgcgg
    10381 tgcctgaccg ggatagctgg ttttaataat cacctgcaca tcagagagat ccggcagtgc
    10441 atccactggc gtgttaatga tggtccaggt gccccagata ctcagaaaca acgcgcccat
    10501 cagcaccaga aaacggttcg ccaccgagcg acgaataatc cattcaatca ttggttattc
    10561 cctcaatgcg catgggtagc actttcagag cgcatccgct ccagtgcgcc agaaatattg
    10621 gcttcggaat caatcaggaa caggccgctg gaaaccacct tttcaccttc cgccagacca
    10681 gagcgtaacg cggtgacgcc ttgcgatgcc tggaaaacgg caacgcgttt cggtacaaag
    10741 cgtccgtcgg catcaacggt aatcacccgc tgttcactgc cggtatcaat cagcgcctgt
    10801 gacggaatga gcagcatcgg ttcgctggcg gtattgagtt gcaaccaggc gttcatgccc
    10861 ggttttagcg actcgtcggc gttgtcgact tccagacgca gctgcagcgt gcgggtcgcg
    10921 gcatccacgc cgggtagcag ggtccatttg cgaatggtga gtgttttatc aggtcgcgcc
    10981 ggaacggtga gcgtaaactg cgaggcatct ttcaccagcc atgcgatgga ctccgggatc
    11041 gcggcagtga tccacaccgg gtccataccc tgaattttcg cgaccacgtt atctttggcg
    11101 atattcattc ccgcgcgcag atcaaacgcg gtgatcacgc catcaatggg tgctttgagc
    11161 gtaaagcgag tctggatttt ttgcgtggca atcaggcggc gaatatccgc ctccggcatt
    11221 cccgccagtc gcagtcgctc aagaatgcct tcagtctggg tcgccgtacc gccggtttcg
    11281 cgcagcagta aatactcact ctgcgcttcc acccagtcgg gaatggtcag gtcgagaagc
    11341 ggtgtgccct tttgtacttt atcgcccacg gtaagcggat acaccttatc gataaacccg
    11401 gcagcgcgag cctgcacaat ggcatactga tactcgttgt aactgacatt cgccgggaaa
    11461 ctctgggcaa aagtcagcgg cccgcgcgtt acggtcgccg ttttcacccc cagattctgc
    11521 gtctgggtcg ggtcaatgcg tacaccagac gcagaactct cttcatcggc atatttcggc
    11581 accagatcca tatccataaa cggcgattta cctggtttat cgaaccgcgt attgggatac
    11641 atcgggtcgt accagaataa gattttacgt tctgcggtcg acgttttttc tgcgggcggt
    11701 tccgcctttg caaaccaggt aaaacctgcc gcagaaataa taccgcccgc gatcatgctg
    11761 ccgataataa gcgcgatttt tttcatctca ttaaacctgg gttactggct gactttaata
    11821 tcctgtaata aagaaaggtt gccctgctgg acaaaattaa acgccacttt gtcgccggtt
    11881 ttaatttcac tcattttcgt ctgcggggtg atggtaaagc gcatggtcat ctccggccag
    11941 ttcaccgcgg taattggatc gtggtggatg atgatttttt tgctttccag atcaatggtt
    12001 tttaccacac cggtggcgct aatgacctgt ggttgtgcct cgctcatggt ttcatgatga
    12061 tgttcgttag cctgggcatt aaagccaata acggtaaaca gactaaacat tgcgacttgc
    12121 agtgcttttt tcatttattc tctcctggag ttaaaaatta ttgctgccaa ccgccgccta
    12181 aggcggtata taaagaaatt tcattaacct gacgggcata attcaggtca agtaaagttt
    12241 gtcgggttgc aaataaagaa cgctcagcat ccagcacttc cagataactt accgcgccgt
    12301 gctgatataa tgcccgcgcc cgttgcaaag taatttgcag cgacgccaga taacgctgct
    12361 gggcgctaat ttgatcgttc aggctttgac gtaatgccag cgcatctgcc acttctttaa
    12421 aggcgttctg gattttctgt tcataattca ccaccgactg ctgctggcga atttcggcga
    12481 tatccagatt ggcctggtta cgtccggcat taaaaatggg gatctcaatt ttgggtataa
    12541 aattccacat cccgctgctg gcgttaaata atgacgacag atcgctgctg gcggttgata
    12601 ttccgctggt caggctgata gacgggaaaa atgccgcgcg tgcagcacca atattggcat
    12661 tagccgccat taacgcatgt tcagcttcca taatatccgg gcgctgcaat aagatttgcg
    12721 acgacaagcc cgccggtaat ttaacgcttt gcaggctgtc gctgtttact gtctgcgctt
    12781 gcggcagctt gccgtagctt cccaataaca gttgcaatgc attattcgcc tgcgccagtt
    12841 ccccctgacg tttagcgatg tcgctgcggg tactttcaat caccccgcga gcctgttcca
    12901 gcgccagaac attactgcta cctgtcaaca gttgtttttc gacaaacgca tatgactgct
    12961 gataattacg cagcgtttct tcggctattt gcaattgcgc atacgccagc tgctgattga
    13021 aatagctttg cgcgacatta gaaaccagca aaatatgcac cgcgcgctga gcttcctcag
    13081 tggctaaata attttggcgc tcggcttcgc tcatgttctt taagcgcccg aaaaaatcga
    13141 gatcaaagct ggcgttaagg ccagtcgaga actcccgcgt cgtggctgaa tcgcctttaa
    13201 gattgttgct ccagctgccg ctgccctcgc cattgagctg tgggtagcgg tcggcatcgg
    13261 tcagacgata ttgcgcccgc gcttcctgca ctttcagcgt cgccatgcgc aaatcccggt
    13321 tattcaccag cgcctcgcta atcagcgtct tcacctgatt atcaacaaaa aaggtgcgcc
    13381 agcccgcgtt ctggtagtta tctgctgcgt taaccaggcc gttctggttg agtgagaact
    13441 gctgcggcac gggcattgcc gggcgctgat aatccggtgc cagtgaacaa ccggttagcg
    13501 caagggccac acaaaatggc agaagtttac aaggagacat aggctcataa tttctggtga
    13561 ttttatgccg ccaactttac tcgccaggct ctgattttcc ggtgacagga aaatgacaaa
    13621 attgtcattt tgccaataag cgattgccat ctgatcccgc tactctagaa ttgcccgggc
    13681 aacatgcgga ggaaatatga aactgttgat tgtcgaagat gaaaagaaaa ccggagaata
    13741 cttgaccaaa gggttaaccg aagccggttt tgtggtcgat ttggccgaca acgggctgaa
    13801 tggctaccat ctggcgatga ccggtgatta tgatctgata atcctcgata ttatgctgcc
    13861 ggacgtgaac ggctgggata tcgtgcgcat gttgcgttcc gccaataagg ggatgccgat
    13921 tctgttgctt accgcgcttg gcaccattga acatcgcgtc aaggggctgg agttgggggc
    13981 ggatgactat ctggtgaagc cgttcgcttt tgctgaactg ctggcgcgag tgcgcactct
    14041 actgcggcgc ggggcggcgg tgattatcga aagtcagttt caggttgccg atctgatggt
    14101 cgatctcgtc agccgcaaag tcacccgcag tggcacgcgc atcactctga ccagtaaaga
    14161 atttactctg ctggagtttt tccttcgcca tcagggcgaa gtactgcccc gctcacttat
    14221 tgcctcacag gtgtgggaca tgaatttcga cagcgacact aacgccattg atgtggcggt
    14281 gaagcggctg cgtggcaaaa tcgacaacga ctttgagccg aagctgattc aaaccgtgcg
    14341 cggcgtgggt tacatgcttg aggtgccgga tggtcagtaa gccgtttcag cgcccgtttt
    14401 cgctggcaac ccgcctgacc ttttttatta gcctggccac catcgcggcg tttttcgcct
    14461 ttgcatggat catgatccac tcagtaaaag tgcattttgc cgagcaggat attaatgatt
    14521 taaaagagat tagcgccact cttgaacggg tacttaatca ccctgacgaa acgcaagccc
    14581 aacgcttaat gacgctggaa gatatcgtca gtggttattc caatgtgttg atttccctgg
    14641 cagatagcca cggtaaaacg gtgtatcact cccccggtgc gccggatatc cgcgagttta
    14701 cgcgtgacgc catacccgat aaagacacgc ggggcggtga ggtgtatctc ctttccggcc
    14761 cgacgatgat gatgccgggc cacggtcacg ggcatatgga acacagcaac tggcggatga
    14821 ttagcttgcc ggttggcccg ttggtggacg gcaaaccgat ttatacgctc tacattgcgc
    14881 tttcgatcga ttttcatctt cattacataa atgatttgat gaataaactt attatgaccg
    14941 catcgatcat cagcatcctg atcgtcttta tcgtgctgtt ggcggtacat aaaggtcacg
    15001 cgccgatccg cagcgtcagc cgtcaaatcc agaacattac ctcgaaagat ctcgacgttc
    15061 gccttgaccc gcagaccgtg cccattgagc tggaacagct ggtactgtcg ttcaaccata
    15121 tgatcgagcg tattgaggat gtctttaccc gccagtccaa tttctcagcg gacatcgccc
    15181 acgaaattcg tacaccaatt acgaatctca taacgcaaac ggaaatcgcc ctcagccagt
    15241 cacgcagcca gaaggagctg gaagatgtgc tctactctaa tctcgaagag ctgacgcgaa
    15301 tggcgaaaat ggtcagcgat atgctgtttc tcgcccaggc cgataacaac cagttaatcc
    15361 ccgaaaagaa aatgctcaac ctggcggatg aagtcggcaa agtgttcgat tttttcgagg
    15421 cattagcgga agatcgcggc gtggagttgc ggtttgttgg tgacgagtgt caggttgcgg
    15481 gcgatccgct gatgctgcgt cgggcgttaa gcaacctgct ttcgaacgcc ctgcgttata
    15541 cgccacccgg agaggcaatt gttgtgcgct gccaaacggt cgatcaccag gtgcaagtta
    15601 ccgtcgaaaa ccccggtacg cccattgcgc ccgagcactt accgcgattg tttgaccgtt
    15661 tctatcgcgt tgacccctcc cgtcagcgaa aaggcgaagg tagcggtatt ggcctggcga
    15721 tagtgaaatc gattgttgtc gcgcataaag gcacggttgc agtaacgtca gatgctcggg
    15781 ggacaaggtt tatgatcaaa ctaccggaga aaggttaatt agttagttca aactcaaaga
    15841 gtgatgtatc gccttctgtt attatttatc aataaaacag ggaagacctt attggtcgtc
    15901 tgaaatcgaa ggttattcat tttcggatat ttacgagata tttagatatg caat
//
LOCUS       DAZGHF010000108        15762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGHF010000108 DAZGHF010000000
VERSION     DAZGHF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.108"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(216..1754)
                     /gene="emrB"
                     /locus_tag="ACHK3A_003615"
     CDS             complement(216..1754)
                     /gene="emrB"
                     /locus_tag="ACHK3A_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO8741649.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(1771..2943)
                     /gene="emrA"
                     /locus_tag="ACHK3A_003616"
     CDS             complement(1771..2943)
                     /gene="emrA"
                     /locus_tag="ACHK3A_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO8741650.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(3070..3600)
                     /gene="emrR"
                     /locus_tag="ACHK3A_003617"
     CDS             complement(3070..3600)
                     /gene="emrR"
                     /locus_tag="ACHK3A_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO8741651.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(3691..4026)
                     /gene="ygaH"
                     /locus_tag="ACHK3A_003618"
     CDS             complement(3691..4026)
                     /gene="ygaH"
                     /locus_tag="ACHK3A_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO8741652.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(4016..4753)
                     /gene="ygaZ"
                     /locus_tag="ACHK3A_003619"
     CDS             complement(4016..4753)
                     /gene="ygaZ"
                     /locus_tag="ACHK3A_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO8741653.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(4877..6061)
                     /locus_tag="ACHK3A_003620"
     CDS             complement(4877..6061)
                     /locus_tag="ACHK3A_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8741654.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRTL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLPWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASVWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AI"
     gene            complement(6253..7245)
                     /gene="proX"
                     /locus_tag="ACHK3A_003621"
     CDS             complement(6253..7245)
                     /gene="proX"
                     /locus_tag="ACHK3A_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO8741655.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(7303..8367)
                     /gene="proW"
                     /locus_tag="ACHK3A_003622"
     CDS             complement(7303..8367)
                     /gene="proW"
                     /locus_tag="ACHK3A_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO8741656.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(8360..9562)
                     /gene="proV"
                     /locus_tag="ACHK3A_003623"
     CDS             complement(8360..9562)
                     /gene="proV"
                     /locus_tag="ACHK3A_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO8741657.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(9918..10877)
                     /gene="nrdF"
                     /locus_tag="ACHK3A_003624"
     CDS             complement(9918..10877)
                     /gene="nrdF"
                     /locus_tag="ACHK3A_003624"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO8741658.1"
                     /translation="MKLSRISAINWNKITDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(10887..13031)
                     /gene="nrdE"
                     /locus_tag="ACHK3A_003625"
     CDS             complement(10887..13031)
                     /gene="nrdE"
                     /locus_tag="ACHK3A_003625"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO8741659.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSSDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDIERVYGKPFADVAISEHYAEL
                     VANERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSSITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(13004..13414)
                     /gene="nrdI"
                     /locus_tag="ACHK3A_003626"
     CDS             complement(13004..13414)
                     /gene="nrdI"
                     /locus_tag="ACHK3A_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO8741660.1"
                     /translation="MSQIVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(13411..13656)
                     /gene="nrdH"
                     /locus_tag="ACHK3A_003627"
     CDS             complement(13411..13656)
                     /gene="nrdH"
                     /locus_tag="ACHK3A_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO8741661.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAANA"
     gene            complement(13903..14232)
                     /locus_tag="ACHK3A_003628"
     CDS             complement(13903..14232)
                     /locus_tag="ACHK3A_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO8741662.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            14384..14728
                     /locus_tag="ACHK3A_003629"
     CDS             14384..14728
                     /locus_tag="ACHK3A_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO8741663.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(14765..15214)
                     /gene="alaE"
                     /locus_tag="ACHK3A_003630"
     CDS             complement(14765..15214)
                     /gene="alaE"
                     /locus_tag="ACHK3A_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO8741664.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         3952 a         4013 c         4061 g         3736 t
ORIGIN      
        1 ggtgcgagtt atggggttaa aagaaaaatg tgagtatcac ccgcgaaagc gagggcgaaa
       61 gaaaaagaga attaacaacc tgagcgaatg ctcagaaccg gttaatggtg aatggaagag
      121 tttggggcgg cgaaaagata caccgaatga gtggataaaa caaccgtttg caaaatgagc
      181 aaataaaaag ggagccgcag ctccctttga caaccttagt gcgcaccgcc tccgccgcca
      241 cctgcaccaa atggcggttt agcaaaccac accagcccca gcaggacgag gaatattccg
      301 gctgacatcc agaagatctc attggcggaa ataatcagcc cctgattagt gatctgctgg
      361 gcaatccagc ctgatgcctg ttgttgcgtc atccccagcc cttccagttg gctgtacatc
      421 gcctgagcgt tcgggttaaa cgggtttacc gactcggtta actgtgcgtg gtgcatcgac
      481 tcgcggttag tccacatggt ggtggtgatg gatgtgccga tagaccccgc cagcgttcgc
      541 gtaaagttgg agaggctcga tgccgccgcc agtcgttccg gtggcaaacc ggacagcgta
      601 atggtggtca gcggcataaa gaagcaggcc accgcaaacc cctggataaa ctgcggccag
      661 gccgacgcgc caaaatccat acctggttca aaggtatagg cgcgccagta gaagcagacg
      721 gcatacataa taaagctgaa ggttaccagc cgccgcatat ccagtttatg cgcgaagcgg
      781 ccgataatcg gcgacaggat caccggaata atccctaccg gcgcagaggc cagacctgcc
      841 cacgttgccg tgtaaccgta gacctcctgc aacaactgcg gcagcagaac aatcgcgccg
      901 aagtagagca tatacgcgag gctgatacac aagcagccga tggtgaagtt gcgcgactta
      961 aacaacgaca gatcgactat cgggttatcg tcggtcagct cccagacaat caggaagcag
     1021 atagccacca ctgccaccac ggtaaggatg ataatttcct gtgatgagaa ccagtccagc
     1081 tctttaccgc ggtcgagcat aatctgcagg ctaccgatac caataaccag cagtgccagc
     1141 cccacggcat caatccgccg ccgttcggtg cgggtttcac gtccgcgcag ggtttgcagt
     1201 gtcatcaaca ccaccgccac gccaatcggt acgttgatga agaatatcca gccccagtgg
     1261 taattgtcac tgatataccc gccgaggatc gggccgcaaa ttggcgcgac aatcaccgtc
     1321 atcgaccaca acgccagcgc gatcgagcgt ttggctggcg ggtagttatt aagcaataga
     1381 ctttgcgaaa gcgggatcag cggcccggcg acaatcccct gaatcacgcg gaagaagatc
     1441 agcatattca ggctgctgga gacaccacac gcccacgacg caatagcaaa ggcgatggtg
     1501 gaccagagga acagtttcac ttccccgacg cgctttgcca gccagccggt aagcgggatc
     1561 gagatggcat tcgccacccc gaaagaagtg attacccacg ttccctggct gagcgatgag
     1621 cccagattcc cggcgatagt ggggatcgcc acgttagcaa tggtggagtc cagcacctgc
     1681 atgaatgtcg ccagtgacag cgcaatcgtc ataatgacca gttgcgcgcc ttccagcggt
     1741 ttttgctgtt gcatcacacg cacctctgga ttagccagcg ttagctttta cgatatcgtc
     1801 gatcagttta ttgacaggtg ccaggctgat ttcacgcgcg gtgcttaccg ctaccggtgt
     1861 ggaacgtact ttatttgcca gtacctgacc gtcacggtta gtggtattga cgctcaccag
     1921 cgtggacaaa ccgatacgca gcgggtattg ctcgagctgt ttctggtcca gttcaatacg
     1981 aacaggcaga cgctgaacga ctttgatcca gttaccggtt gcattttgcg ccggaagcag
     2041 tgagaacgcg ctacctgtgc ccatatccag accaaccact tttccggtgt atttcacatc
     2101 atcgccgtaa atatcggtgg tgatggtgac cggctgaccg atacgcatat tggcaatctg
     2161 cgtctcttta aagttggcat ccacccacat attggtggct ggaacgaccg ccatcagcgg
     2221 cgtcgttggg ctaatttgcg ccccaggctg taccgcgcgg cgggagacat aaccggtcat
     2281 cggactgaca atacgggtac gctccagcgc cagccaggcg ttacgtactt cggtggcagc
     2341 ctgttgcacg gcaggctgat cttccagttt agtccccaga atcatcgcct gattggcatt
     2401 gtattgttga atagcgacgt ccagttgtgc ctgggcactg gtaacggcgt cgcgggcgtg
     2461 ttgcagctct tcgcgaccaa tcaggttggc attgcccagc ggcacacggc ggttgtagtc
     2521 gctttgtgct ttcgcgaggg cgattttttg cacctcaata ttcgcctgca actgtttgct
     2581 gttaatcatc agctggtggg tttggcgaac gctggaagcc agtgcagttt tggctttttc
     2641 aaacgcctgg cgagcatctg tcgggtcgag agtgaccagc acatcgcctt cttttacaaa
     2701 atcggtgtta tcggcccaga ctttcgtcac gctgccagac acctgagaca taatttgaat
     2761 ctgattccct gccacgtatg cgtcatcagt ttcttcgaaa tgacgcagta ccaaaaacca
     2821 ataaatccct atcgctacgg caataattat aaagagcaag gtgagaagga ggagcagacg
     2881 cttacgtttg ccgctcttct ttaccggttg ctgcggggtt tgagtctccg catttgcgct
     2941 catattgttc tccacgatct tcttaactca tcggctgagc cgacctgctt gccaaaaagg
     3001 ccagcatgtt gcgatgctgg ccagtcattt tttcttttat aaatctggat ttttgagcga
     3061 gatgacgcgt tagctcatcg cttcgagaac cacaccgtct tgttccatct ggtcgagacg
     3121 ggagagcaat ttgcgggtga tttgctcgag ctgatctttt tctgttgtgc tgagcgcgga
     3181 ccagagttga tgcaggcagt tatgctgcgg tggtaaaacc tcgcgcaaaa actcgtgacc
     3241 tttttccgtt aattgcagat gcaggcagcg gcgatcgtta tcgctttcac gacgttcgat
     3301 ccaaccgcgt ttttccagtt catcggcaat acgcgtcgcg ttggtacggg atgatccaag
     3361 agcacaactc aattcagaag gctgaatact gtggttttcc tgagactcca gcgtaatcaa
     3421 cgccataaac aacgtctcgt taatcccctg agccttcagc attttattgc ggttctccag
     3481 cagcttgctt tgcatgtgca tgcaaagacg ggtcagaagg atctcctgat aaggaaaatc
     3541 ttcgtggcgg ctggcgcgaa attttagcat ttgttcaatg ggcgtaaacg aactatccat
     3601 ttgggtatga cctcattaat ttcagccgat atagtaacga cagtgacaaa taaagtaaat
     3661 gtattgtttt agaaaaatga ttcttgtggg ttatataatc gccatcactt tccaggcgag
     3721 cccataggcc agcgcactaa gcagtgttgg gatgataatg ctgcgtgttt tatagaaact
     3781 ggcacccagt accgcgaagc cgaccagagt gggcacgaaa cggcgtgtat cgtgcatcac
     3841 ttctggtgcg gtagagacaa ccagcagagc gcatatcgag gcgatgccaa tggtgtcgag
     3901 caaaatacct accgcgccac gtttggttgg gcgggcatta cccacacgca ggcgcagcgg
     3961 caaatagcgg aagcaataat tcgccgcgcc aaccagtaac ccaagcagca gaacctcata
     4021 gctcatcggg cgctccttgc cagaatgcct ggattaacgc agtgaggcag ccacagacaa
     4081 tgcctgccag aatggcgacg ggaatagaaa atagcgttac gcctgcaagg gcaccaacta
     4141 acgctgcggt aacgcaaaga gattgtttgc gctggaaaga ggcgagcagg aaactcataa
     4201 agagtgccgg aagcataaaa cctaatgctg cttcaacggc gggatatcct tgcagcaagc
     4261 cgctgccgga gaatgcccct attaccgtac caaataccca cgatgaccat gaactgaagg
     4321 caatgccgat catccagttc tcgctccagc ggcgattatt gcgtaccagt tttgcggttg
     4381 cggcggcaaa aacctcatcc gtcaggccaa acgcccacag ggcagttttc gatttttgca
     4441 gacgctgaat aatacggcta cgcagtgacg ggccatacaa aacatggcga acatccattg
     4501 ccatgacggt cagtgcagca acccacaaac tactcccggc tgccagcatc gcggtaatga
     4561 cgaactggct cgcgcctgca taaatgatgc aggagaaaaa aacgctttcg agaggagaga
     4621 atcccagacg ggtcgcattc agaccgaacg caaaggccac cggaatataa ctaataacaa
     4681 tcggtaaact gtctttgcat ccttccatga aggtcgccga accaggagca ggctgtggag
     4741 tagggctttc catagagtgt acgcttaacg attgttaata ttataaatag actgaatgaa
     4801 tatcttaacc ttatcagact gatgggcttc ttaacaccct tataagtgta aagccacgaa
     4861 aacggttgct gattgcttaa attgcggctt cctgacgatg aaaacctcgc caccagacca
     4921 gtaagttaac cagggcaatc gtcgcgccag ccagacaaac gccagcccaa ccgccatgtt
     4981 gccagactga ggctgaaatt agcgaaccgg cggcaccgcc aatgaagtag ctggtcatgt
     5041 aacctgcggt caggcgatta cgcgcatcag ggtgtattcg ataaattacc gtctggttag
     5101 tgatatgcac gccctgcacg gtgagatcca gcaccaggat gccgataatc agcgccagta
     5161 cggaagtgtg accaaaccag atcgccagcc atggaagtaa cagcagcagc agaccgaaag
     5221 ttgtggtgtg gtgcgatttg cccttatcgg caaatccgcc cgccggacga gcgcccaacg
     5281 ctccggctgc tcccgcaagt ccaaacagac caatgacgcc atcgctgtaa ttaaaaggcg
     5341 gagcggcgag caaaaaggcc atcgaggtcc agagaatgct gaagttggca aaggtcaggc
     5401 agcccagcaa cgtgcgggtg cgcagaattt tatcgctgat aaacatgctg aaaacggaac
     5461 caagcaactg tgggtagttg aggtgggttt ctgatttcat ttgtggcaga ccacgccata
     5521 atgccagcgc catcagtgcc attaacaccg aagcaaccca aaagacggtg cgccagccgc
     5581 cgaggtttgc cagcaacccg gcaacggttc gtgccagcaa gatccccaac agcagcccgc
     5641 tcataatggt gccgaccact ttgccgcgtt tgtccggtga agccagcgtc gctgccagcg
     5701 gaaccagaat ttgtgccacg accgagaata aaccggttaa tgccgtaccg aggatcatca
     5761 tcgccagcga ctgactgctg gcagtgatta acatgccgca tgccgccagt aaggtcatcg
     5821 agacaatcag gcggcggcgt tcaaacatat caccgagggg aacaagaaac agcagacctg
     5881 cggcatagcc caactgcgcg gcggtaacaa taaagcctgc cgaactggcg gaaagggaaa
     5941 agttacgcgc gatggtgtcg agcaatggct gggcgtaata gttactggct accgccaggc
     6001 cggtggcgat agacatcagc acgatcagcg ccgggctaag ctcatgatta ggtttagtca
     6061 ttatttttgt tcatcaggtg atgattaagc tggaaatcaa taatagcgga tgttgatgaa
     6121 tatgggatga aagatagatt gttgaaatgt gcggtgtggt ttgtaggtat gataagacgc
     6181 accagcgtcg catcaggcat tgtgtacaga gcggcggatg aggcaaaaac gccttatccg
     6241 cccgaataaa aattacttct gcgctgccag cgcctcattc acccagccat cgaactgctg
     6301 ctggtgggct ttgatccagc catcaacgtg gccctgaata tcgccttctg aggctttgcc
     6361 gtcatgcata atggcgttct gggcgttaat atcagccact ggtaactgca taatggcaaa
     6421 cagtttcgct gctgccgggt ttttctcggc ccaggctttg ttggcaacga tatgcatggt
     6481 actgaccggg aagccgtaat tcgcgccatt cggcagtttg gtatcggcat ttttatcgcc
     6541 cggcagtgcg gagaacggca cctgcaacca gaccacatct ttccccggtt tcagttcgtt
     6601 actcacccag tacggcgtcc aggtgtagta aaacaccggc ttaccctctt tgtaacgact
     6661 gatggtgtca gccatcatcg ctgcgtagtt cccctgatta tgcgtcacgg tattggtcag
     6721 tccatacgcg gcaagctggt ggttgatcgc accttcgcag ccccagcctg ggttacagcc
     6781 ggttaaatcc gctttaccgt cgccgttggt atcgaacagt ttggcgatct tcggatcttt
     6841 aagttgcgcg atgttggtaa ttttgtactg gtcggcagtt ttcttatcga tcaggtaacc
     6901 ctgtgccgcg ccgttaacaa atactccttc acgataaaat ttcttatcgc caccggcagc
     6961 ttcgtacatg ttgtcatgca gtggcgtcca gttcactgcg gtgaaggttg catcgccgga
     7021 agcaagcgag gtgtagccaa cgttgtaatc cacttcgctg ggtttattga cggtataacc
     7081 taatttctcc agcgcgcggc tgaccagcag cgtctggaaa gtttcttcgg tgatggtgct
     7141 ctgaaccgga ttaacagtaa tgcctttgcc cggcaggtcg gcagcaaaag tttgtgtaga
     7201 gataagcgtg gcaaacgctg tcgcaaaaag tacgctatgt cgcattgtta ttcctttttt
     7261 tggcagggtg ataatgcccg gcgtcaccgg gcaagtgcag agttacttaa tgaatgggcg
     7321 ggtcagcaga ccgacagggc cagtggtgta ccagcgacgg ttgccgcgac tgcgtgagtc
     7381 gcgcccaacg gcctgcgtca ggcggtcgag gataatggcg aggatcacaa tcccgacgcc
     7441 gccaacggtg gcaagcccca tatccagacg accgataccg cgaagcacca tctgacccag
     7501 cccgccgacg gcaatcatcg aggcgatgac caccatagaa agggccagca tcagcgtctg
     7561 gttaacgccc gccataatgg tcggcatcgc cagcggtaac tgaactttga acagcatctg
     7621 gcgcgggctg gcaccgaatg agcgcgaggc ttcaatcaga tccgccggaa cctggttaat
     7681 tcccagaatc gtcagacgga taatcggcgg cagcgcaaag atgatcgtca ccaccacgcc
     7741 cggcacgtta ccgataccaa acagcatgac gattggtacc agataaacaa acgcgggcgt
     7801 ggtctgcatg gcatcaagca gtggacgaat aattttcgcc gctcgcggac ttctcgccag
     7861 ccagatcccc aacggcaaac cgatgacgat acagaacagc agggcggtta gcaccagcgc
     7921 cagagtaacc attgcctgtg accaggcacc gattgcgccg atggcaatca gcgaaaccag
     7981 tgtcgccacg cccattccga caccggaaat ttgccaggcg atgagagcga aaacgataat
     8041 cgccaccgga gcgggcatac ccagcagcaa ttgctggaaa ccgttgagga tgtaatcaac
     8101 cggaacgcgc acgccctgaa agacgggacg aaaatgggta acgacccagt cgatcccttc
     8161 agtgacccaa ctgtcgagcg ggatcagcgt tttatggaac ggatcgagaa tattaaaatg
     8221 ctcgacgttt ggcgcaggcg tactggtcag ccagtcagca ccaccgccgt cagtcggtgc
     8281 agcagccggt gtaccccagg cgtctgcgga ctgtgcggca ctgtccgccg ctggcgtggt
     8341 atcccacgga ttattttgat cagccattat ttaccccctc acgatctaaa gcgcgcagca
     8401 gcattccttt cgaaatgatg ccgacatact gttggtcctc atcgaccacg ggcaccgcac
     8461 agggggcctg tccgacatga gagagcaact cgctaagagg cgtttgtgca tcgaccgcta
     8521 acggcgcatc aatcagcgcc gcatcaagac cttgctgctg cgttaacgcg gctttaagcg
     8581 aatcgataga cacagcgccg acaaacttat taccgcgttc gatgacgtag ccatattcgc
     8641 gatcttcatc ctgcaataat ttcagtgccg aacgcgggcc gaagccgggg gttttacgaa
     8701 ttaagccgtt cggtgtacga cgggcaatat ctttcgcact gaacacctgg ctaatatcga
     8761 cgccacggaa gaaggtacgg acataatcat tcgccggatt attgagaatt tcatccggtg
     8821 tgccgacctg tactacttca ccattttgca taatggcaat tcggtcgcca atacgcatgg
     8881 cttcatcgag atcgtgggaa ataaagacaa tggtgcgctg atgtttcgcc tgtaatttta
     8941 ccagctcatc ctgcatctcg gtgcgaatta atggatcgag cgccgagaag gcttcgtcca
     9001 ttaataatat atccggatta atcgctaacg cgcgggctaa tcccacacgt tgacgcatcc
     9061 cgccagagag ttcatccggg tagctgtggg cataattttc cagcccgacc tgacgcagtg
     9121 catcaagggc tttttcacgg cgttcttcgg cattaattcc ggccagttcc ataccgaacg
     9181 cagtattgtc cagcacggtc atatgcggca ttaaggcaaa ggactggaag accatcgcaa
     9241 tcttttttct gcgcacctca cggagttcgg cgtcggatat tttggcaata tccacaccat
     9301 caatcagcac ttgcccgcgg gtgggttcaa tcaggcgatt gagaaggcgt accattgtgg
     9361 atttacccga accggataat cccatgatga caaatatctc gccttcttca atggccagac
     9421 tggcgtcttt aacgccaagc gatagcccag ttttttccag aatttgttct tttgaaagtc
     9481 cttgttcgat atatttgaaa gctcgctgtg gatgctcgcc aaatatttta taaagatttt
     9541 taatttctaa tttaattgcc atgcaataga aagattcctg ttatttgttt atgtcgatac
     9601 attacccaat ggaaatagtc acttttttct accctaacat actgagaatc tgaggcaacc
     9661 cctgatggca aatgtggcat gaatattgat gtgagcaaat cgccggaatt tcccatgata
     9721 taagggctga gagcaaatcg agaaaagttg atattttttg tcaaagatag caaaaaaacg
     9781 cctgaatatt gtggtttcag gcgaataatg caacagagta tcagtgtaga tcaccacaaa
     9841 ttatttgcgt gatataaagc tatttggcgg gaattatttc cctgcagcgg gtagtgatat
     9901 ttttgaaaat aaccccctca gaaattccag tcttcatctt ctgtttcgac cgcttttccc
     9961 atcacatatg aagagcctga accggaaaag aaatcgtgat tttcatcggc attcggcgaa
    10021 agcgcggcga ggattgccgg attcacttcc gccatttctg cgggaaatag cggctcgtag
    10081 cccagattca tcaacgcctt attggcgttg taacagagaa acgctttcac atcgtcagcc
    10141 cacggggttt cggcgtacag ctcatcggtg tattgcaact cgttgtcata gagttccagc
    10201 aacaaatcga aggcgaaact cttcaattct tcacgtcgtg cctgggagat cttttccagg
    10261 tttttctgat atttatagcc gatgtagtaa ccgtggactg cttcatcgcg gataatcaga
    10321 cggatcaggt ctgcggtatt ggttagcttt ccgcggctgg aaaaatacat cggcagccag
    10381 aaaccggaat agaacaaaaa agattcaaga aacacactgg cgattttctt tttcagcgga
    10441 tcatcaccgc gataatgttg ctgaataatc tgtgcttttc gctgcaacgg cgcgttttct
    10501 tcactccagg cgtaggcggc atcgacatct ttggtctggc acagcgtcga gaaaatcgaa
    10561 ctgtaagagc gggcatgaac cgcctccata aagctgatat tcgataatac tgcctcttca
    10621 tgaggcgtga gcgcatcggg catcagcgaa ggcgcgccga taacattttg cagcgtgtcg
    10681 agcagtgtca ggccagtaaa aacgcgcatc gtcagttgtt gttctacgac acttaatgtc
    10741 tgccaggcgg gaatatcgtt cgatagtggc accttttctg gtagccagaa attgctggtc
    10801 aggcggttcc acacctccag atctttatcg tctgttatct tgttccagtt gatggcgctg
    10861 atacgtgaga gtttcataga tattccttaa agtgcacagg agacgcagcc ttcaatttca
    10921 gtgccttcca gcgccatctg acgcaggcgg atgtagtaga gcgttttgat acctttgcgc
    10981 caggcgtaaa tctgcgcttt gttgatatcg cgagttgtgg cggtatcggg gaaaaacagt
    11041 gtcagcgaca gcccctgatc gacatggcga gtcgcttcgg cgtaggtgtc gatgatcttt
    11101 tctgcgccta tttcgtaagc gtcctgatac agcgccagat tctcgttagt cataaacggg
    11161 gcaggatagt aaacgcgtcc tgttttgccc tctttgcgta tctctacttt cgccacaatc
    11221 ggatgaatac tcgacgtggc atggttgata taagagatag aaccggttgg cggcaccgcc
    11281 tggagatttt ggttgtatat gccatagcgc atcacgtcgt cgcgcagttg cgcccacatt
    11341 tcgcgggtag gtaacgtgat gctgctacga gcaaacaact cgccaacttt cgccgttttc
    11401 ggttgccagt tactttgcag atattggcta aaatattcac cgctggcata gcgcgactgt
    11461 ttgaacccgg caaaggtttc gccgcgttcg cgcgccagca acatcgaggt acgcagtgcg
    11521 tgccaggtga tggtatagaa atagagattg gtgaaatcga gtgcttccgg cgatccataa
    11581 gcgatgcctt ctcgcgccag ataaccgtgt aaattcatct gccccagtcc gatggcgtgt
    11641 gaggcggcat ttccggcttc gatggacggc acgctgcgga tatgactcat atctgacacc
    11701 gccgttaaac cgcgcacggc agtctctacc gtgcgggcaa agtcggggga atccatggtg
    11761 tgggcaatat tcaacgaacc taaattgcag gaaatatcat ggcctgtgcg ggcatagtcg
    11821 agattctcgt catactctga ggcgctgtta acctgcaaaa tttctgagca gagattgctc
    11881 atatttatgc gcccggcgat agggttagca cggttaaccg tgtcttcata catgatgtac
    11941 ggatagccgg actcaaactg gatttctgcc agtcgctgga agaaatcacg ggcgttgatg
    12001 tattttttgc ggatgcgttc attggcaacc agttcggcat agtgttcact gatggcgaca
    12061 tcggcaaatg gcttgccata aactcgctct atgtcatagg gcgaaaacag cgccatctgc
    12121 gcattctctt ttgccagatg gaaagtgata tccgggatca ccacgcccag cgacagtgtt
    12181 ttaatgcgga ttttttcgtc ggcattttcc cgttttgtat cgagaaaacg cagaatatcg
    12241 ggatgatgag catgtaaata gactgcgcca gcaccctgac gcgcgccgag ctggttggca
    12301 taggaaaatg cgtcttccag cattttcatc accggaatca cgccagaaga ttgattttca
    12361 atacgtttaa ttggcgcgcc cgcttcccgc agattcgaca gcaaaaatgc aacgccgccg
    12421 ccgcgtttcg acagttgcag tgcggaattt actgcccggc caatcgactc catattgtct
    12481 tcaatacgca gcaaaaaaca ggaaaccagt tcgccgcgct gctgcttacc acagttgagg
    12541 aatgtcggtg ttgcaggctg aaagcgtcct gacagcatct catcggtcag ttgcgtcgcc
    12601 agtgtttcat cgccttgtgc cagcgtcagc gccaccatcg tgactcgatc ggcaaaatct
    12661 tccagataac gcttaccgtc gaatgtcttc aacgtatagc tggtgtaaaa cttccatgct
    12721 ccgaggaatg tctgaaaacg aaaaccgctg gcgtgtgcgt gggcaaacag cgtaatgaca
    12781 aaatcgctag agtagcgatt aaggacactt tcatcatagt aaccttcgtt aaccagccag
    12841 ttcaggcgct gctgctggct actgaaggcc acactgttcg gacgcacatg cgtcgtcata
    12901 aaggcgtcta ctgcctggcg atctttatcg aactgaatgc gacctgcgct atcgtaaagg
    12961 ttaagcatcg cattcagcgc gtggtaatcc atagtttcct gcgtcaggcg ttctgcggtt
    13021 gtcgttgcca aaattcggtt actcctttac gaacgttttc gatatcgctt tgcgtaccca
    13081 tgagttcaaa gcggtacagc catggcacgc cgcatttccg ggcaatcaca tctccggcgc
    13141 ggccatatgc ctcaccaaag ttgcgattac ccgaagcaat aacgccgcgc agcaacgccc
    13201 ggttatgctc gtcgtttaaa aagcgaatga cctgtcgtgg caccgcgcca gccgtaccgc
    13261 cgccgccgta agagggcacg atcaggatgt aaggctcgtc tacctgaatc cgttcccgct
    13321 cattgagcgg gatgcgcacc gcgggcagcc ctaaacgttc gataaaacgc tgcgtatttt
    13381 cggagctgct ggaaaagtag acaatctggc tcatgcattg gccgcgtgtg gtgctggatg
    13441 cagacggttg atcatgtccg ggcggaaacc tgaccagcta agatcgcctg caatcacgac
    13501 cggcaactga cgaaagccct gagcacgcag cgcttccgcc gcttcaggaa cgcgatcgac
    13561 attaatcatt tcaaagtcaa agccccggtt ttccatcgcc cttttggtgg cgtggcactg
    13621 aacgcaatcg ttacgagtgt aaatagtaat gcgcatgatt cgtatttccg tttaaaatga
    13681 agatacggcg cgatgatacg cgtcgggttg tctctctgtt gatacagaaa tactagatgt
    13741 agttgaaaaa agattcaacc acacaatata tagcaaatag tggtcgaaat taccctgggt
    13801 atgagcgtga cggggtaggg ggatttttgt gattcaccag gcaaaaagaa accccgaagc
    13861 caggcttcgg ggttaaagac gcgtatttat tatcatgttg cactacgatt tgcgcatgct
    13921 taacagtgcg ccgataaaaa tacctaccgc agctgctgta cccacgctac accagggtct
    13981 ttcacgaaca aaagaatccg cgcagccaac ggcatcgcgc gcggcttgct ggacgcgagt
    14041 acgaccatgc attcgcgctc tggtttcttt cagtagagcc tgcgctttac tacgagcggc
    14101 ttcggcttcc cctttggcgt cgctgcccca ggacttcaat acagattcga gactatcagc
    14161 taattgattg acatcattct ggatatcctg cacgccatca tcaacgtcgt tgcggttcgg
    14221 tcggttaaac atatgatccc ccattagaat tcgatgtaat tttcagtgta gaccataatt
    14281 ttcttaactg aggttcatcg cagctttatg gacttttctg aaagcgttgt gaatgcgcaa
    14341 tcaggtaagg taggcccgcg gttgataata acgaggatca aatatgtatt tacgaccaga
    14401 cgaggtggcg cgcgtacttg aaaaagtcgg ttttactgtc gatgtggtaa cccagaaggc
    14461 ttatggttac cgccgtgggg aaaattatgt ctatgttaat cgcgaagcgc gaatgggacg
    14521 taccgccctg gtgattcatc caacattaaa agaacgtagt tcaacactgg ctgaacccgc
    14581 ttccgatatt aaaacttgcg atcattatca acaatttccg ctctatttag caggcgagcg
    14641 acacgagcat tacggtatcc cgcatggctt tagttcgcgt gttgcgcttg aacgttattt
    14701 gaatggttta tttggcgaag ccagttaaag acgcgactgg cgatgccagt cgcgaaaaga
    14761 agggtcaggc ttttacctgc tggtaacggc tgactttaaa cagtcggcgg caataatcga
    14821 ggaagtagcc ataaaccgcc cccatcaaca tcgaaacaac gatgtttgaa ctgaccgccg
    14881 ccataatctg atgccagtct gcgcccacca ctaacaagat cgccacatac accggtgact
    14941 ggaacgtcac ataagccatg atatccgcca gattttttat ccagcccgac gggctaactt
    15001 tgcgtgctgc gcgcataaac agatcgcggt acataccgta tggccatgca attaagatgt
    15061 ttaccggaat cgctaccaat ctggaataaa aagactgttc gaagctcatt ccggagagga
    15121 aaacttcaat acacatgttc acgacagaac agtaaacaac catcgcgaac gtatctgcaa
    15181 ctgcatgacg caagcgtgac tgcggtgaga acatgctaat gctccttaat aaaagcgtaa
    15241 tgggatgtga atggagatga aagcagtgga tctcattgcc atctgcattt ttgcatagcg
    15301 tatatcgctg gctatgacta atcaattagt gaaaaatttt tatttaatct gtttttgtag
    15361 ataaaatatt ccgtagcggc gcgttttttg tacattgtgg atttttacat gccgatatga
    15421 tttattttta attaaatcaa ttggatatgt gtttaccttt attttcactc atgaaggttt
    15481 gggggggggt aacgctataa gcgtagagaa ttcggtgtat gtagtgatcg ggtcattttc
    15541 agcatttttt atgccagata atgtacattg acagcagaaa gcaccagtga tagatgtttt
    15601 cttttattct gttgagttgc atttttattc actttggttt tgtcgggctt gcggaattag
    15661 cgagcagaga gcgccgctct gttcaccaat aacctaaatt actcatcaaa atggctaata
    15721 tataatcttg aaattattct gagggtttca gaaaataaca gc
//
LOCUS       DAZGHF010000109        15606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGHF010000109 DAZGHF010000000
VERSION     DAZGHF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.110"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..563)
                     /locus_tag="ACHK3A_003631"
     CDS             complement(<1..563)
                     /locus_tag="ACHK3A_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741665.1"
                     /translation="MSIDFTPCMINTYHGDIYNRTTDTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYTSDQYDLAQYQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAEYRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFITEQRADLPTNIKDIQSL"
     gene            complement(1144..2145)
                     /locus_tag="ACHK3A_003632"
     CDS             complement(1144..2145)
                     /locus_tag="ACHK3A_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO8741666.1"
                     /translation="MSSWLAMLQDATEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            2252..2548
                     /locus_tag="ACHK3A_003633"
     CDS             2252..2548
                     /locus_tag="ACHK3A_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO8741667.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(2671..3096)
                     /gene="arsC"
                     /locus_tag="ACHK3A_003634"
     CDS             complement(2671..3096)
                     /gene="arsC"
                     /locus_tag="ACHK3A_003634"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO8741668.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIVYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQGAFTKEDGEKVVDEAGKRLK"
     gene            complement(3109..4398)
                     /gene="arsB"
                     /locus_tag="ACHK3A_003635"
     CDS             complement(3109..4398)
                     /gene="arsB"
                     /locus_tag="ACHK3A_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO8741669.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVILHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLFLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTSKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLTALALRLSFTL"
     gene            complement(4453..4806)
                     /gene="arsR"
                     /locus_tag="ACHK3A_003636"
     CDS             complement(4453..4806)
                     /gene="arsR"
                     /locus_tag="ACHK3A_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO8741670.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLSLLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQVIVRN
                     LARQNCSADSKNICS"
     gene            complement(5054..5269)
                     /locus_tag="ACHK3A_003637"
     CDS             complement(5054..5269)
                     /locus_tag="ACHK3A_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741671.1"
                     /translation="MMAISLQSEPPTQGWKVKNRRKVGFLHPDSDKAWYGSDPMKVYK
                     SSFTNMYSKLSLRQLKKSLTLAMLATG"
     gene            5548..5631
                     /gene="dinQ"
                     /locus_tag="ACHK3A_003638"
     CDS             5548..5631
                     /gene="dinQ"
                     /locus_tag="ACHK3A_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO8741672.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(5685..7037)
                     /gene="gorA"
                     /locus_tag="ACHK3A_003639"
     CDS             complement(5685..7037)
                     /gene="gorA"
                     /locus_tag="ACHK3A_003639"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO8741673.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7109..7951)
                     /gene="rlmJ"
                     /locus_tag="ACHK3A_003640"
     CDS             complement(7109..7951)
                     /gene="rlmJ"
                     /locus_tag="ACHK3A_003640"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO8741674.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLSSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            8154..10196
                     /gene="prlC"
                     /locus_tag="ACHK3A_003641"
     CDS             8154..10196
                     /gene="prlC"
                     /locus_tag="ACHK3A_003641"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO8741675.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILIRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            10204..10956
                     /gene="rsmJ"
                     /locus_tag="ACHK3A_003642"
     CDS             10204..10956
                     /gene="rsmJ"
                     /locus_tag="ACHK3A_003642"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO8741676.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11005..12474)
                     /gene="dtpB"
                     /locus_tag="ACHK3A_003643"
     CDS             complement(11005..12474)
                     /gene="dtpB"
                     /locus_tag="ACHK3A_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO8741677.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(12789..13223)
                     /gene="uspA"
                     /locus_tag="ACHK3A_003644"
     CDS             complement(12789..13223)
                     /gene="uspA"
                     /locus_tag="ACHK3A_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO8741678.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            13614..13949
                     /gene="uspB"
                     /locus_tag="ACHK3A_003645"
     CDS             13614..13949
                     /gene="uspB"
                     /locus_tag="ACHK3A_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO8741679.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14020..15519)
                     /gene="pitA"
                     /locus_tag="ACHK3A_003646"
     CDS             complement(14020..15519)
                     /gene="pitA"
                     /locus_tag="ACHK3A_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO8741680.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
BASE COUNT         4027 a         3898 c         3988 g         3693 t
ORIGIN      
        1 tacaggcttt gaatatcttt tatattagtc ggtaagtcag ctcgttgttc agtaataaaa
       61 ttagtatctg aaaacatggt ttggacactg aatcttactg gtattccctc ttctacgcta
      121 attttagtta tgattgcctt gtcgggttta ggattagcga caacaatgat tcgatattca
      181 gcacttttgt ttttttcgta tctaatgtta ttaatctgat taagtattga ttgtgtctgt
      241 tgcgtaagtt gtgtcgaact tatcgtttca atgttagtaa taagacttac aagttgagcc
      301 acaacttttg agtagtctgc atttgtatat gtagtgttcg tgtggcacgc attaatttgt
      361 tgaagttggt attgcgctaa atcatattga tcagaggtat atccttctcc agagaatgtg
      421 gaattaatgg gttgttgttc ttcccaatta tcgcaaggcc acttcggtgt gtctggtgtt
      481 ttaacattgt cggtatctgt tgtgcggtta taaatatcgc cgtgatatgt atttatcata
      541 catggggtaa agtcaattga cataacattc cttattgttc atctatttcg ctaaattttt
      601 gtaaatcgga gaattagagc aggaatggat tttcttctac gcggaaataa acacaatgcg
      661 tcatatcttc ctgatataat tacctttaac ttccctttca tttccacccg gtaatatttg
      721 tgtataaaaa aagggcagtg atagtatcgt aaaggaaaac gttaacaact atcaaaaagc
      781 gctcatttta aaaagttatg atgttcaccg atagtgtgtg aagtgatatt tttgtgatgt
      841 tttagatgtg taataatgaa ccaggttaaa gtgtatttaa aaatgtgttg tattggcgac
      901 tgaaatctgc cctttaaaag gggggatagc actgaaacaa agagacattg cttctccgat
      961 aaaatgtcag agaagcatga ttgcgtggtg attgtcgatt gataacttac tgacggatcc
     1021 gtcatcattc tcaaagcatt agttaagtta tttgtgattt ttaaggatta tcctatctat
     1081 gattttctac tcagaaggat aaggcactca caatgctcct tatagtttac tgctggcatc
     1141 tatttaaaat agcatttgag tcaaatagcc catcaagata gccataccta atataaccgt
     1201 caggaatgca actatcatcg gtattctgaa cattgattta agtaatatca cttccgtcag
     1261 gcttgcgccg gcactgccga tgattaatgc cattaatgct cccagaccca ttccttttgt
     1321 catcagaaca gatgccagag ggataaccgc ctctgcccgg atatatagcg gaataccgac
     1381 aacggcgctc aatgggatgg cgaagggatt atctgcccct gcatgagcgg caatccactc
     1441 tgagggaata aagccataaa taaaagagcc tattaatacc cctaacaaaa gatatggcag
     1501 tacatcttta aactgttgca acgcatcttt ggtggctagt tttatgggat taatacttac
     1561 cacagctttg gtactgcaac agtttactac aggtttctca atggctttag ccgctggact
     1621 acagcaactc acttttattg gcgtatatgt cgtccccggc gaagttttgg ctggagcaca
     1681 acaatttgct gatgagcttt tactggcaat gatatgacgt tcaaatccca gagaatcgag
     1741 gataatactg gcaagtacgg agacgccggc ggcgataatc gcatacaaca aggtaacttt
     1801 ccagccaaag gtcacccaca ttaacccgac gataatggga ttaagtaatg gggaaacaaa
     1861 taaaaaagtg agggtcggac caaacccggc tttcgctgat aacaatccac gtaacatagg
     1921 gattgtcgag caactacaga acggggtgac ggctcccaac agagcagcca ggagatagcc
     1981 tttccctttt cttgccccca tcatctgctg gattttatgg tctggcacct tttgtcttat
     2041 caggctcaca ccggcactaa tcactataaa cagcaaagaa agctcgacgg cgagaaacac
     2101 aaacatctct gtggcatctt gcagcatcgc aagccaacta ctcatacata acctcattga
     2161 ataaatctat atttccagaa taatcgaaat gaaattcaga tcaagatatt tctggtatta
     2221 tcgaaatatc aaccagacag agggggatca tatgcagttg gaagaagtcg ctaaagcgct
     2281 gaaagagcta ggtcacccga cccgtttgtt catatttaaa catcttgtca aagccgggga
     2341 acaagggttg ccggtcggtg agttgcaaaa gcagcttggg atacccggct ctacactcag
     2401 tcaccatatt tcagcactgg tatcggtagg gctggtcaaa caaaaccgtg agagtcgaac
     2461 gctgatgtgt gtttcgcaat acgcaatgct ggaagcgata attgaattct tacgcgagga
     2521 gtgctgcgta aatagcaaaa ttgcctgatg cgctacgcgc tacagatttc atgccatgca
     2581 cggttttgta ggtcggataa gacgcgttag cgtcgcatcc gacataaaca aagcgcactt
     2641 taccaactat gggcagtgga tatcgccgct ttatttcaag cgtttacccg cttcatcaac
     2701 cactttctcg ccatcttcct tggtgaacgc gccttgttgc gcatctggca gaatttccag
     2761 cacgacttct gaagggcggc acaggcgagt tcccaacggc gtcaccacaa tcgggcgatt
     2821 aatcagaatc gggtgctgaa gcataaagtc gattaaccga tcgtcagtaa atttatcttc
     2881 tgcaaggccc agttcctcat acggctcgac gtttttacgc agcagcgcgc gtacggaaat
     2941 ccccatatcg gcaatgagtt tgaccagttc atcacgcgtc ggtggggttt ccagataata
     3001 gacaatggtc ggttctgtgc cgctgttgcg gatcatctcc agcgtgttac gcgacgtgcc
     3061 gcaggccggg ttgtgataaa tggtaatgtt gctcatatca atatctcatt acaaagtgaa
     3121 agagagacgt agcgccagcg cagtcagcgt cacaaacagc acaggcaggg tcatgataat
     3181 ccctgtacgg aagtaatatc cccagctgat agtcatattc ttctgcgaaa gtacgtgcag
     3241 ccagagcagc gtcgccaggc taccaatcgg ggtaattttc ggccccaaat cgcagccaat
     3301 tacgttggca taaaccatcg cttctttgat aacgccagat gccgtgctgc catcaatgga
     3361 caacgcgcca accagtaccg tcggcatatt gttcataata gaagagagga aggcggtgag
     3421 gaatccggtg ccgagcgtcg cggcccacag gccattatcc gccagcacgt tgagtacgcc
     3481 agaaagatat tccgttagtc cggcattgcg caggccataa accaccagat acatgccgag
     3541 cgagaagatg acaatctgcc agggcgcacc gcgtagcacc ttactcgtat taatcgcatg
     3601 accgcgttta gcgacgacaa ataatatcaa cgcgcccact gccgcaatgg cgctgaccgg
     3661 aatgccgagc ggttccagga cgaaaaatcc caccagcaga aacagtaaaa caacccagcc
     3721 agttttgaag gtggcaggat ctttaatcgc ttctgcggga gatttcagca gcgccatatc
     3781 gtagttctgc ggaatatctt tgcggaaata gagatgcaaa atcaccagcg tggcaacaat
     3841 tgcggcgata tccaccggca ccatcaccga tgcgtattcg cgaaagccga ggccaaagaa
     3901 atctgcggaa acgatattca ccaggttgga gacaataagc ggcaggctgg cggtatcggc
     3961 aatgaatccg gccgccatca cgaacgccag cgtagtgcct ttactgaacc ctaaagccag
     4021 caacatggcg atgacaatcg gtgtcaaaat aagcgccgcg ccatcattgg caaacagagc
     4081 tgcaacggca gcaccgagca ggacaatcca ggtgaacagc aagcgaccgc gtccattgcc
     4141 ccagcgtgag acgtgcagcg ccgcccattc aaaaaagccg gactcatcca gcagcaggct
     4201 gatgataatg acggcaataa acgccgccgt cgcgttccag acgatattcc acaccaccgg
     4261 aatatcaccc ggatggacca cgcccgtaac taacgccagt actgcgccga gcgttgcact
     4321 ccagccgatg cctaaacctt tcggctgcca gataaccaat acaatggtca ggacaaagat
     4381 agcgcctgcc agtaacataa tgcctcccgg ataaaacaca tgcgaaaata catatgtgtt
     4441 tagctaaatt ttttaactgc aaatgttctt actgtctgcg gaacaatttt gtcgagccag
     4501 gttgcggaca atcacctgaa ccttttcctg ttcacatcgc caggcctcat caataatttt
     4561 cgccgcccat gctggaatat gcggtgataa gcggtaatga acccacttac cttgcttgcg
     4621 atccagcaat agcccacttt cacgcagcaa tgacagatgg cgggatatct tgggctgcga
     4681 ctgatcgagg gcagtgcaga gatcgcagac gcataactct cccagttcgc taagcagtaa
     4741 aacgatgccc agacgggtct catcagcaag aattttgaac agttgaatgg gtaacagaaa
     4801 tgacatattg cgctcctgat tgttgcaggt agtgtctctc ttcgaagcgg ataagtcaaa
     4861 aacatatatg gtttaacgaa tgtgtaagtg cagaggaagg taataggtgt gaattttgag
     4921 ttgggtattc atttgaaagg aggtctgaat caggacgtaa aaaaaccgac tttgcgtcgg
     4981 ttttttactt tccagccctg agttggtggc tctggctgga gtgagaatgc ccacatctaa
     5041 cggttcaata ctattagcca gtggctaaca ttgcaagcgt tagcgatttc ttcagctggc
     5101 ggagggacag tttgctatac atgttggtga aagaggattt atatactttc atcgggtcgc
     5161 tgccgtacca ggccttgtcc gaatccggat gtaaaaaacc gactttgcgt cggtttttta
     5221 ctttccagcc ctgagttggt ggctctgact ggagtgagat agccatcatc taacgcatca
     5281 atgctaatac catactgaaa ctattgcaag gacatgctgg ttttataacc cgcaagtact
     5341 gtatgattat ccagttagct ctgagacatt ttcactctgg caatgcgcat aaacgctttc
     5401 aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg gagtgagaat
     5461 gcccacatct aacaatgggg catgcgccgt gacaggcagt ggatgagtaa gcggatgcat
     5521 tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt tcttggggcg
     5581 ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg atagcggaaa
     5641 cgtagctaag ggctaagagc acactactct tagcccttta acatttaacg cattgtcacg
     5701 aactcttctg ccgctgtcgg gtgaatggca accgtgttgt cgaagtcttt tttggttgcg
     5761 cccatcttca gcgccaccgc gaagccctgc aacatttcat ccataccaaa accaatgccg
     5821 tgaattccga caatcttctc ttccggtcca acgcatacca gtttcatgcg gcacggctgg
     5881 cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac tttcacctga
     5941 tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtccc aatcggcgga
     6001 tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg cttgttatta
     6061 aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag ttccactgca
     6121 cccgtgttat cgcccaccgc gtaaatacct tcaacgttgg tattttgata tttatcgacg
     6181 acgatatagc ctttttcgtt agttttaacg ccagcggctt ccaggttgat gttgtcattg
     6241 gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg cccatcttcc
     6301 agctccagcg tcaggctacc atcagcattt ttcactactg ctttcgggat ggcgttggta
     6361 tgcaactgcg ggccttcggc gttcatcact tcgaccagcg tttcagagat catcgggtcg
     6421 aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc gaggccgtta
     6481 atcacgcccg ccagttccac ggcgatgtaa cctgcgccaa caaccgccac gcgctctggc
     6541 aaagcaggaa gggcgaagaa gccatcagaa tcaataccgt attccacgcc tggaatatcc
     6601 gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat ggtttcgccg
     6661 tttacctcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac atcaacgtta
     6721 tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc ggtacggctg
     6781 gcgatcaacg tttcccagtt gaatttattg atagtggtat cgaaaccata atccggaccg
     6841 tacatgtgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt cggcacgcag
     6901 ccgacgttca cgcaggtgcc gcccagttct ttggcttcaa tcagcgcgca tttctgaccg
     6961 tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat ggcgatgtaa
     7021 tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt gtagcgcgag
     7081 acgtaatagg tgccagcgat ggctgcaatt actccggcac gatccagctt gcggtggcgt
     7141 gcccagtgcc agctggaacc agtttactgt gcagccacgg cagcacgtta ttcatctgct
     7201 gttccagctt ccacggcgga ttaatcacaa tcatgccgga agcggtcatg ccacggcgat
     7261 cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg gtcgcttcca
     7321 ggtcgtggat catgcgctta atttgctggc gcagcaccac tggataccac aatgcgtaag
     7381 tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc tgatagtcgg
     7441 ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgggaaacc ggcggcagct
     7501 tggccttaag ctgctggaaa ccgtcggctt tttcgacgcg cgcacggctg tctttctgaa
     7561 attcagaacg cagcaacggg taatcgctcg ggtgcagctc ggtcagttgc aggctgtcct
     7621 gttcacgcag tagctggcga gcaatcaacg gcgaaccggg gtaataacgc aactgcccgc
     7681 tacggttgaa gtgttttacc acattgatgt acgcctccag ctccgcaggc aaatcgtcct
     7741 gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca tgttcgctgc
     7801 ttaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt ttatctttct
     7861 ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg gcgtggttgc
     7921 cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta agtaaaaatc
     7981 ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag aggccgccgc
     8041 cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc tacacacatt
     8101 atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg ctaatgacga
     8161 atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg gaacatgtcg
     8221 ttccagccgt gactaaggcg ctgaatgact gccgcgaaaa cgtggagcgc gtagtagcgc
     8281 aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcagaagta gacgatgtgc
     8341 tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc ccggaactgc
     8401 gtgaagcgta cgaacaaacc ctgccgctgc tgtcagaata cagcacctgg gtagggcaac
     8461 atgaagggct gtataaagcg tatcgcgacc tgcgcgatgg cgatcattac gccacgctga
     8521 acacggcgca gaaaaaagcg gttgataacg cactgcgcga cttcgaactc tctggcatcg
     8581 gtctgccaaa agagaaacaa caacgctacg gtgagattgc cacccgtctt tccgagctgg
     8641 gcaaccagta cagcaacaac gtcctcgatg cgacaatggg ctggaccaaa ctcgttaccg
     8701 acgaagcgga actggcagga atgccagaaa gcgcactggc tgcggcaaaa gcccaggctg
     8761 aagcgaaaga gctggaaggt tatttgctga cgctggatat cccaagctac ttgccggtaa
     8821 tgacttactg cgacaaccag gccttgcgtg aagagatgta tcgtgcttac agcacccgcg
     8881 cttccgatca aggcccgaac gccggtaagt gggacaacag caaggtgatg gaagagatcc
     8941 tcgcgctgcg tcacgaactg gcgcaactgc tgggttttga aaactacgcc tttaaatccc
     9001 ttgccactaa aatggcagaa aatccgcagc aggtgcttga tttcttaacc gatctggcaa
     9061 aacgcgcgcg tccgcaaggt gaaaaagagc tggcgcaact gcgtgccttc gctaaagccg
     9121 aatttggcgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa aaacaaaaac
     9181 agcatctcta cagcatcagt gacgaacagc tgcgtccgta cttcccggaa aacaaagcgg
     9241 ttaacggctt gtttgaagtg gtgaaacgta tttacggcat caccgctaaa gagcgtaaag
     9301 atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgag aacaacgaac
     9361 tgcgcggcag cttctacctc gacctgtatg cccgtgaaaa caagcgcggc ggggcgtgga
     9421 tggatgactg cgtaggccag atgcgtaaag ccgacggttc gctgcaaaaa ccggtcgcgt
     9481 atctgacctg taacttcaac cgcccggtaa atggtaaacc ggcactgttt acccatgacg
     9541 aagtgatcac cctgttccat gaattcggtc acggcctgca ccatatgctg acccgcattg
     9601 aaactgctgg tgtatctggt atcagcgggg tgccgtggga tgcggtcgaa ctgccgagtc
     9661 agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctcc ggtcactatg
     9721 aaaccggcga accgctgccg aaggagctgc tggataaaat gctggcggct aagaactacc
     9781 aggcggcgct gtttattctg cgccaactgg agttcggcct gtttgatttc cgcctccatg
     9841 ccgagttccg cccggatcag ggagcgaaaa tcctcgaaac tctggcagaa atcaagaaac
     9901 tggttgccgt agtaccgtct ccatcctggg gccgtttccc gcacgctttc agccatattt
     9961 tcgccggtgg ttatgccgca ggttactaca gctacctgtg ggccgacgtg ctggcggcag
    10021 atgccttctc gcgttttgag gaagagggca ttttcaaccg tgaaactggg cagtcgttcc
    10081 tcgacaacat tctgatccgt ggcggttcag aagagccgat ggagctgttc aaacgcttcc
    10141 gtggtcgtga accacagctg gatgcgatgc tggagcatta cggcattaag ggctgaacat
    10201 tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt atctgttctg
    10261 gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt gttaacaccg
    10321 gagcacctgg aactgcgcaa gcgtgatgag ccgaagctgg gcggcatctt tgttgatttt
    10381 gttggcgggg cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggaga ggcggtggcg
    10441 aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac cgcagggctg
    10501 gggcgcgatg cctttgtgct ggcttcggtc ggctgccgcg tgcggatgct ggagcgtaat
    10561 ccggtggttg ctgcattgct cgacgacggc ctggcgcgtg gttatgcgga tgcggaaatc
    10621 ggcggctggt tgcaggagcg gttgcagtta attcacgctt ccagcctgac ggcgctgacc
    10681 gatattactc cgcgcccgca ggtggtctat ctcgatccga tgttcccgca taagcagaaa
    10741 agcgcgttgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc ggatcttgat
    10801 gccgatggat tgctggagcc tgcacgcttg ctggcgacca aacgcgtggt ggtaaagcgc
    10861 ccggactacg cgccgccgct ggcgaatgtc gccacgccaa acgcggtagt gaccaaaggg
    10921 catcgctttg atatttatgc cggaacgccg gtgtaaaagt aaagcagggt agcgttatcg
    10981 ctaccctgct ttctgcaaga ataattaatg gctttcgggc gtcgcaatca tgcgtttcag
    11041 ccacggcacc atcagcagca tcaccaccgc aacgcccagc gtaaccaggc caatcttacc
    11101 aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt ctggtactgc
    11161 ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca ggaaccacat
    11221 cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg ccagacccag
    11281 ggcgctaata aacaattcac ccaggctctg gaacaagtaa accagcacga taaaccacgg
    11341 cgatgtcagt ccttgtgcat cagcaaacca cattcccgca gcggcggctg taagaaagcc
    11401 cagcgagcac ataaacatgc cgagagtaaa tttcatcggc atcgagaggt ctttgccttt
    11461 gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca cccagaacgg
    11521 gttaagcgcc tggaagctga ctgggttgat ggaaaagccg agaatttcat gatgcacgtt
    11581 gttgatagca aagaagttca gcgatgttgg catctgggcg tagagaatgt aaaacaccac
    11641 cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt tatccagctt
    11701 gaatgcctga cgaaagaaaa tgatggtgac gacgatggag agaacaatca gcaccagatt
    11761 ggcgacttct acgttgtgca tcagccatgc acagacgaag atcatcacca cgctgccaag
    11821 taacacgtac aacagtttgc tgaagctcat cggccggaag tcgggttcag aaccaatgtc
    11881 tttcaccatt ccgcgacagg cgatgtaaac caatagcgcg ataattaatc ccgcaccgca
    11941 caggttgtag gtgactgaat agccgaatct atcagcgatc acaggggcca gcgataacgc
    12001 tatcaacgag ccgatgttga tcgacatata gaacagggta aatgcgccat caagccgcgg
    12061 atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa acaggccgtt
    12121 accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta gcgacaggcc
    12181 ggtcatgaag tagccaatcg ccagcacaag tgctccgagg acaatggtgc gtttggtccc
    12241 cagcaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca gcgcagcaaa
    12301 agcaccaaaa gtgacaaaag cctgttcttg cgagaatcca agctgcttaa cgaagaaaac
    12361 cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga caaaaaagat
    12421 catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat tcatatttat
    12481 taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt
    12541 tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac tattacaaca
    12601 gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa tgttgtattg
    12661 gcaggggatt ttgattgcgt acgcagaccg tggccagata aggtgtttac gctgcaaggt
    12721 aacaaggcgc gccaaaaagc aaaacgcccg catatgcggg cgttcaggaa cacgtcgtag
    12781 agggaagatt attcttcttc gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg
    12841 ttgatcagct gacgtgcgga agacatcagt ttgctccaga agtcctggtg gtgaccacaa
    12901 accaccaaat ccatatcgta tttcttgatt gcatcgacca gaacctggcc caggtcgccg
    12961 ctgccgctca gggtttcagt gattgggtag cctgcattag tggaaagctc ggtcagcgca
    13021 tgatgtgtct cttcagagat gcgtttctgc atatcaccca gattcacatc aataagcccg
    13081 gtgtataggt cagagtagtt tacatctacg tggatcagag aaactttcgc attgtagggg
    13141 cgagccatag agactgcttt ctctaccaga actttgcttt ccggggagag atcgaccgcg
    13201 atgagaatgt gtttataagc catagtgtta ctccttccat aaagttgtcg atgactggcc
    13261 agctagcgtt tcttgtgccg ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta
    13321 acccagccgt aaagattcag tgttaagacc atccttacct tatagcgacc cggatgatcc
    13381 gtcaatccgc cttgcttacc cgttagataa acaaattttc gacatgaata tattgatagt
    13441 ggttaacctt ctggaaaaaa aacaacctga tctcctacac tatctataga gccgctcgga
    13501 tgttggtcac cacaaaagcg gtttctccag gacctggtgt taactgattg gcagcgtatc
    13561 gggagcggta gccccggggt ggaactccgc gggcaggtcg ccggggagga ggtatgataa
    13621 gcaccgtcgc attattttgg gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt
    13681 tctcatcact acgcgcgttg ttagtggtac tgcgtaactg cgatccattg ctctatcaat
    13741 atgttgatgg agggggcttt tttacctcac atggccaacc caacaaacag gtgcgtctcg
    13801 tttggtatat ctatgcccaa cgttatcgcg atcatcacga tgatgagttt attcgccgct
    13861 gtgagcgggt gcgtcggcag tttattctga ccagcgcatt gtgtggtctg gtggtggtca
    13921 gtctgattgc attgatgatt tggcattaag cacttcagca aaaataacgc ataaaaaaag
    13981 cgggccagta aactgacccg ctcgttttgg tgcgtgcgat tacaggaact gcaaggagag
    14041 ccagtacagc ccgccggaaa gcagtaccgc agccggaagg gtaaacaccc aggccatcag
    14101 aatactggtc acggttttac gctgtaagcc gccgccatct accaccatcg tccccgcgac
    14161 agaagaggag agtacgtggg tagtggaaac cggcatcccg gtataactcg ccaggccgat
    14221 agacactgcc gcagtcatct gggcagacat tccctgagcg taggtcatgc ctttcttacc
    14281 gattttctca ccgatagtcg ttgccacacg acgccagcca atcatcgtac cgatacctaa
    14341 cgccagcgcg accgccatga tgatccacac cggtgcatac tcgatggtgc taagcatgtc
    14401 agacttcagt ttcttcaaca ggcgctgatc gtcagcactc acgccaggca tcttcaccac
    14461 tttgtcgata gtgtcagaaa cgcacagcat aatgcggcgc atctggctac gttgatcaag
    14521 cgacagctta tcgtagcttt ccacatcggt agtcaacatg cctttcaggc ggttgagcgc
    14581 gttaatggta ttcgacggat ggcaatggaa ctccgcaggt tgcgttgcgc cagcttccgg
    14641 agcaggtact aactgatcag caccggtcgc ttgtttcagc agcgcaggat gctgctcaaa
    14701 gtaagcttcg acgttgttga tggcatcacg ggtacgggtg atttcgtagc cagtggcatt
    14761 catgttcacc acgaagcctg ctggtgcgac accaatcaat accaacataa ccagaccaat
    14821 gcctttctga ccatcgttcg cgccatgcga aaacgccacg ccgatagcgg aaaggatcag
    14881 cgcaatacgc gtccagaacg gcggcttttt cttgccgtct ttcttttcac gctccgctgg
    14941 ggtcaggtgg atacgggcgc gtttcttggt gccgctccag tagcgacgca gcaagaaaat
    15001 cagaccgcca gcaaacacca ggccgacaat aggggaaacg atcagagaac cgaaaatact
    15061 taatactttc gggatattga gtgcatccac cactgacgtc ccggtcatca acgcattggt
    15121 taaaccaatc ccgatgatcg cgccaatcag cgtatgagag ctggatgcag gtaaaccaaa
    15181 gtaccaggta cccaggttcc agataatcgc cgccagcaac atagagaaca ccatggcaag
    15241 gccatgagac gatcccatat taagcagcag atccgtcggc agcatatgca caatggcata
    15301 ggcaacactc agaccaccca gcaaaacacc caaaaagttg aacaccgccg ccataaccac
    15361 ggcgagctga gaacgcatcg cgcgggtata gataacggtt gccacggcgt tggctgtgtc
    15421 atggaaacca ttgatggctt cgtagaacag cacaaaagcc agtgcaagca ataataacag
    15481 cccggtatgc aaatccaggc cagcaaacaa atgtagcata ggacgttacg ccattttgag
    15541 gacatgaacg cggcgcatta tcagtgactt tcgcggcgcg ggcaaagtga aatatagact
    15601 tttttt
//
LOCUS       DAZGHF010000110        15498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGHF010000110 DAZGHF010000000
VERSION     DAZGHF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.111"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(100..831)
                     /locus_tag="ACHK3A_003647"
     CDS             complement(100..831)
                     /locus_tag="ACHK3A_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741681.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERVWRDPAEMIVISYSTTVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(977..2953)
                     /gene="speA"
                     /locus_tag="ACHK3A_003648"
     CDS             complement(977..2953)
                     /gene="speA"
                     /locus_tag="ACHK3A_003648"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO8741682.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2962..3093)
                     /gene="yqgB"
                     /locus_tag="ACHK3A_003649"
     CDS             complement(2962..3093)
                     /gene="yqgB"
                     /locus_tag="ACHK3A_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO8741683.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(3187..3330)
                     /gene="yqgG"
                     /locus_tag="ACHK3A_003650"
     CDS             complement(3187..3330)
                     /gene="yqgG"
                     /locus_tag="ACHK3A_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO8741684.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(3441..3692)
                     /gene="yqgD"
                     /locus_tag="ACHK3A_003651"
     CDS             complement(3441..3692)
                     /gene="yqgD"
                     /locus_tag="ACHK3A_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO8741685.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            3748..4902
                     /gene="metK"
                     /locus_tag="ACHK3A_003652"
     CDS             3748..4902
                     /gene="metK"
                     /locus_tag="ACHK3A_003652"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO8741686.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            5339..6733
                     /gene="galP"
                     /locus_tag="ACHK3A_003653"
     CDS             5339..6733
                     /gene="galP"
                     /locus_tag="ACHK3A_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO8741687.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            6810..7307
                     /locus_tag="ACHK3A_003654"
     CDS             6810..7307
                     /locus_tag="ACHK3A_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO8741688.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            7402..8109
                     /gene="endA"
                     /locus_tag="ACHK3A_003655"
     CDS             7402..8109
                     /gene="endA"
                     /locus_tag="ACHK3A_003655"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO8741689.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            8189..8920
                     /gene="rsmE"
                     /locus_tag="ACHK3A_003656"
     CDS             8189..8920
                     /gene="rsmE"
                     /locus_tag="ACHK3A_003656"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO8741690.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8933..9883
                     /gene="gshB"
                     /locus_tag="ACHK3A_003657"
     CDS             8933..9883
                     /gene="gshB"
                     /locus_tag="ACHK3A_003657"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO8741691.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9992..10555
                     /locus_tag="ACHK3A_003658"
     CDS             9992..10555
                     /locus_tag="ACHK3A_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO8741692.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            10555..10971
                     /gene="ruvX"
                     /locus_tag="ACHK3A_003659"
     CDS             10555..10971
                     /gene="ruvX"
                     /locus_tag="ACHK3A_003659"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO8741693.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(11145..12125)
                     /locus_tag="ACHK3A_003660"
     CDS             complement(11145..12125)
                     /locus_tag="ACHK3A_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO8741694.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            12143..12847
                     /gene="yggS"
                     /locus_tag="ACHK3A_003661"
     CDS             12143..12847
                     /gene="yggS"
                     /locus_tag="ACHK3A_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO8741695.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLDVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            12865..13431
                     /gene="yggT"
                     /locus_tag="ACHK3A_003662"
     CDS             12865..13431
                     /gene="yggT"
                     /locus_tag="ACHK3A_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO8741696.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            13428..13718
                     /gene="yggU"
                     /locus_tag="ACHK3A_003663"
     CDS             13428..13718
                     /gene="yggU"
                     /locus_tag="ACHK3A_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO8741697.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            13726..14319
                     /locus_tag="ACHK3A_003664"
     CDS             13726..14319
                     /locus_tag="ACHK3A_003664"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO8741698.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLIYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            14312..15448
                     /gene="hemW"
                     /locus_tag="ACHK3A_003665"
     CDS             14312..15448
                     /gene="hemW"
                     /locus_tag="ACHK3A_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO8741699.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3752 a         4093 c         3957 g         3696 t
ORIGIN      
        1 ccttccgcgt cgttaggccc gacgaggaag ggttggattt gcgaaacaca attttgtgct
       61 aattatcgcc ttaaatgggc gaggcatcaa gcgaggttgc tatttccgac acggtggcgg
      121 cgcatagcca ttgttatctg ccggaaaggc ttgtccagag cacggcggca gcgcgttttg
      181 cgccgggcct tgtaattcaa aggcggcaaa gccgattaca ccggtattac gcacatcgcc
      241 ttgcgccgaa ttggctgcgt aggaatcagc aacgttggcg aactgaaaag ccgcttcggt
      301 atgtttatct ttgcggaatc ctttaatcgc taacgaatct ccggcattca caatatagcc
      361 gttgttattc agcgaacctt gcttgccgtt cagcacatcc aggccatcaa ccgtagcaac
      421 gatctcgtag ttggtattac gactgtaatt acggatatat aattgataat tcattccgac
      481 tctggcatgg acaatatagc gtggttcatt attggatgaa tcataaaccc gcgtaatagg
      541 tagagaatta aaattaccat cgcgaatagc atattccagt tcgttaatac gaatgctata
      601 aacgcggtca tagccggaag gaacagtagt cgaataacta ataacgatca tctctgccgg
      661 atcgcgccag actcgttcag cagatacgcc ctgaaccgat gagtgtacat ccccacccca
      721 ggcggtgccg acattgcctg atgcactgct attatactgc gcattactgg aacaaccggc
      781 cagcagcacg gctaatgcta tcaacgcgct acgtactttc cattttttca taaccgttcc
      841 ctctgcattt aattaatgca gcgattgtag ggggattatt taataagaaa agcagctaaa
      901 ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa tcgacccgcc
      961 agcggaataa tcttaattat tcatcttcaa gataggtata accgtacaaa cctgcctcga
     1021 actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc acttgatcac
     1081 ggaactgggt taacaacgtt ttcggatcga gctgtacata ttgcagcata tccgccacgg
     1141 tatcgccttc gtcagacagt tctacttcta cgctgccgtc agggaagacg aacacgtcaa
     1201 ccgcttcggt atcaccgaac aggttgtgca tgttgccgag gatctcctga tatgcgccga
     1261 ccatgaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt ggcattgtcg
     1321 tggcaatacc atcaccatcg atgtagtgat caatagcacc gtcagaatca caggtaatat
     1381 ccagcagcac agcgcggcgc tccggcactt gatccaaccc ttccagcggc agaaccggga
     1441 acaactggtc gatcccccat gcgtccggca tcgactggaa cagcgagaag ttgacgtaca
     1501 ttttgtcagc catacgttcc tgcagctcgt cgataatcgg acgatgcgca cggttttgcg
     1561 gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct gcccatgcac
     1621 gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatatcgtgc agatccatct
     1681 ggctgtcgtg taaccattca cgcagagaac gacgagttcc cggttcgtgc atctcctgcc
     1741 aggtttccca catgctttgc agggcgcgcg gtgcgtcttc tgcaggcgcg gtcggcaccg
     1801 tgtattcgtt acgttccacg ccgatgatat tcgacaccag cacggtgtga tgcgcagtca
     1861 ccgcacgacc cgactcggtg attaccgtcg gatgtggcag accattttct tcacacgcat
     1921 caccaatcgc ccagatgatg ttgttggcgt attcattgag gccgtagttc accgaacagt
     1981 cggactgcga acgggtgcct tcatagtcca cgcccagacc gccgccaacg tcgaagcact
     2041 gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca cgaacgcctg
     2101 tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagc agttgcaggc
     2161 tgtcgagacg cccggcttca cgcagcgttt caaccagttg cagtacctga gtcgccgcca
     2221 ggccgaactt cgatttttcc ccgccagagg actgccattt acccgagccc tgcgaagcca
     2281 gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca tccagcacaa
     2341 tggcaatttc tgacatcttc tcaatgacca gatagacctt gtggcccatc ttctcgccaa
     2401 ttaatgccag gcggatatat tcgcggtcct tataaccgtt gcagacgatg acgctacggg
     2461 tcatgccagc atgtgccagc accgccatca actccgcttt ggaaccggct tccaggccca
     2521 gcggttcgcc cgaatgaatc agggactcaa tcacgcggcg gtgctggtta actttgatcg
     2581 gataaacgag gaagtaatcg ccgttatagc cgtaggattc ccgcgcacgt ttgaacgcgg
     2641 cgttaataga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt gcaggcagac
     2701 gctggccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga gcttccggga
     2761 cgtccgggtc cgggcacacg ctaatgtggc ccagctcgtt aacgtcatag tagttattgc
     2821 cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag ctcattgcaa
     2881 cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa cccatagaca
     2941 tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt taacgactat
     3001 cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca gtgaatgctg
     3061 acgctccaac tgcgcaaccg gtttcttttt cataacatta ttaagcacat aaccgaacgt
     3121 aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag cgcccttgtt
     3181 cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat gggtataaca
     3241 tcggcaggta tgcaaagcag agatgcagag tgcggggaac gaatcttcac cagaacggtg
     3301 agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg gccgcctgtt
     3361 aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc aacacgaaag
     3421 aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac aatggcagaa
     3481 aattgcaaaa gataaatacg cagaatgcca gcattgtcag gaaaaatttc cagccacgtt
     3541 tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa ttgacctaaa
     3601 atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc agtgttttta
     3661 acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag tttgagctaa
     3721 ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc cgagtccgtc
     3781 tctgaagggc atcctgacaa aattgctgac caaatttctg atgccgtttt agacgcgatc
     3841 ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac cggcatggtt
     3901 ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat cacccgtaac
     3961 accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa ctcctgtgcg
     4021 gttctgagcg ctatcggcaa acagtctcct gacatcaacc agggcgttga ccgtgccgat
     4081 ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac caatgaaacc
     4141 gacgtgctga tgccagcgcc tatcacctat gcacaccgtc tggtacagcg tcaggctgaa
     4201 gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca ggtgactttc
     4261 cagtatgacg acggcaaaat cgttggtatc gatgctgtcg tgctttccac tcagcactct
     4321 gaagaaatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat caagccgatt
     4381 ctgccggctg aatggctgac ttctgccacc aaattcttca tcaacccgac cggtcgtttt
     4441 gttatcggtg gcccgatggg tgactgcggt ctgactggtc gtaaaattat cgttgatacc
     4501 tacggcggca tggcacgtca cggtggcggt gcattctccg gtaaagatcc atcaaaagtg
     4561 gaccgttccg cagcgtacgc agcacgttat gtcgcgaaaa acatcgtggc tgctggcctg
     4621 gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc gacttccatc
     4681 atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct gctggtacgt
     4741 gaattcttcg acctgcgccc atacggtctg attcagatgc tggatctgct gcacccgatc
     4801 tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg ggaaaaaacc
     4861 gacaaagcgc agctgctgcg tgatgctgcc ggtctgaagt aatctttctt cacctgcttt
     4921 caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggac aggcattcac
     4981 gccgcatccg gcaaaaaaac cgcccgcaca ataacatcat tcctcctcaa cacgttttac
     5041 cgcaaattac catcactact gaaaccgatt acaccaacca cgccagccaa atatttgtaa
     5101 tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt aaagtcttaa
     5161 ttgcagataa cagcgtttaa tctatgatga tataactcaa ttattttcat gcacttaaat
     5221 cataaccaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct tcacatcttt
     5281 ttacgtcgta ctcacctatc ttaattcaca ataaaaaata accatattgg agggcatcat
     5341 gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg tctgcttcct
     5401 tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg gcgcactgcc
     5461 gtttattgca gatgaattcc agattacttc gcacacgcaa gaatgggtcg taagctccat
     5521 gatgttcggt gcggcagtcg gtgcggtggg cagtggctgg ctctccttta aactcgggcg
     5581 caaaaagagc ctgatgatcg gcgcaattct gtttgttgcc ggttcactgt tctctgcggc
     5641 tgcgccaaac gttgaagtac tgattctttc ccgcgttctg ctggggttgg cggtgggtgt
     5701 ggcttcttat accgcaccgc tgtacctctc tgaaattgcg ccggaaaaaa ttcgcggcag
     5761 catgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt atctttctga
     5821 taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta tcatcccggc
     5881 aattttgctg ctgattggtg tcttcttcct gccagacagt cctcgttggt ttgccgccaa
     5941 acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca gcgcggaagc
     6001 gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg gctgggcgct
     6061 gtttaaagag aacagcaact tccgccgcgc ggtgttcctt ggcgtactgt tgcaggtaat
     6121 gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct tcgaactggc
     6181 gggttatacc aacaccaccg agcaaatgtg ggggacggtg atcgtcggcc tgaccaacgt
     6241 actcgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac caacgctaac
     6301 gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga tgcatatcgg
     6361 tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt ttattgtcgg
     6421 ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc agccgctgaa
     6481 aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca acatgatcgt
     6541 tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct tctgggtgta
     6601 tgcggctctg aacgtactgt ttatcttgct gacattgtgg ctggtaccgg aaaccaaaca
     6661 cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc gcgaaatcgg
     6721 cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccacc tcttgcagtc
     6781 atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct atcgccatcc
     6841 agcaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg aagctagggc
     6901 gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc ggaacagcct
     6961 ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac agtgaagctt
     7021 ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa catttcggcc
     7081 gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg ggcgttcccg
     7141 cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc ccctaccgct
     7201 gcaagtgcca ggagcatcag cttaccgtac gccgccataa tcgcgtagtt cgtggcgagg
     7261 ccgtctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca cctgaactat
     7321 caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac gttgctggct
     7381 cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt ggtactgagc
     7441 gcagcatttt ccggcccggc gttggccgaa ggtatcaata gtttttctca ggcgaaagcc
     7501 gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg taaaattaac
     7561 tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt gcgcaaaaat
     7621 gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca attcggtcac
     7681 cagcgccagt gctggcagga cggtggacgt aaaaactgcg ccaaagatcc ggtctatcgc
     7741 aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gtgaggtgaa tggcgatcgc
     7801 ggcaacttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca atgcgccatg
     7861 aaggtcgatt tcaaagaaaa agccgccgaa ccaccagcgc gtgcacgcgg tgccattgcg
     7921 cgcacctact tctatatgcg tgaccaatac aacctgacac tctctcgcca gcaaacgcaa
     7981 ctgttcaacg catgggacaa gatgtatccg gttaccgact gggagtgcga gcgcgatgaa
     8041 cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg ccaggcgcga
     8101 aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg cgtacaaccg
     8161 cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc atcctgaacc
     8221 actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata tcgggcgcgt
     8281 actgcgtatg gggccggggc aggcgttgca attgtttgac ggtagcaacc aggtctttga
     8341 cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtactgg aaggccagat
     8401 cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc gtggtgaaaa
     8461 aatggaattt acgatccaga aatcgatcga actcggtgta agcctcatta cgccactttt
     8521 ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc tccagcagtg
     8581 gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc cggaaatccg
     8641 tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga aactgaatct
     8701 tcacccgcgc gccagtaaca gcatcaatac gttgccgtta ccggttgaac gtgtccgcct
     8761 gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg cccgctatca
     8821 atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg cgctcaccgc
     8881 cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa taatgatcaa
     8941 gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt ccagtttcgc
     9001 tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga tgggcgatct
     9061 gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga agcagaacta
     9121 cgaagagtgg ttttcattcg tcggtgaaca ggatctgccg ctggccgatc tcgatgtgat
     9181 cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct atattctgga
     9241 acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc gcgactgtaa
     9301 cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg ttacgcgcaa
     9361 taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta agccgctgga
     9421 cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc tcggcgtgat
     9481 tgccgaaacc ctgactgagc atggcactcg ctactgcatg gcgcaaaatt acctgccagc
     9541 cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac cgtactgcct
     9601 ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg gtcgcggtga
     9661 acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc cgacgctgaa
     9721 agaaaaaggg ctgatttttg ttggtctgga tatcattggc gaccgtctga ctgaaattaa
     9781 cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt cgatcaccgg
     9841 aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct tagcgagaag
     9901 gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg gcgctgttgc
     9961 ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact ttcttattgc
    10021 catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt gcgaacataa
    10081 taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca aaattgaagg
    10141 gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc gtctggataa
    10201 accggttatg ctcggtggtc cgctggctga agatcgcggg tttattttgc atactccgcc
    10261 ctctaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca cttcccgcga
    10321 tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg ctctgggcta
    10381 tgcctcctgg gagaaaggtc aactggaaca agaaattctc gataacgcgt ggctaacggc
    10441 cccggcggat ctgaatattc tgttcaaaac accgattgcc gaccgctggc gcgaggcggc
    10501 aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcagggcacg cctgatgagt
    10561 ggaaccttac tcgccttcga cttcggtacc aaaagcattg gtgtagcggt cggccaacgc
    10621 attaccggca ccgctcgccc attgcctgca attaaagcac aggacggtac gccggactgg
    10681 aacattatcg agcgtttact gaaagagtgg cagcctgacg aaatcatcgt cggtttgccg
    10741 ctgaatatgg acggcaccga acagccgtta accgccagag cgcgtaaatt tgccaaccgt
    10801 attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag caccgtggag
    10861 gcacgttccg gtctgtttga acagggcggc tatcgggcgc tcaacaaagg caaagtcgac
    10921 tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta aggcgattta
    10981 ctagcgcaca gatttctgac aatgtgctcg ttgtttcatg ttggatgcgg cgtaaacgcc
    11041 ttatccaacc tacagtgagt gcgaattcaa agttacgctt cccttgtagg cccgataagc
    11101 ttgcgcatcg ggcatattta caattcgtat cagacacagc aacgtcacaa gcgcccttct
    11161 ttcacccgct gctgataact ctgctgaaac gttatcatcc ccacctgctg cccggtttga
    11221 ataacatgcg gtaactggtg ggttttccct tcacgaatca aattccccac cgcgggtgtg
    11281 ttaatcagca attcaaacaa cgccacgcgt ccttcctgtt tatccacttc cagcttttgt
    11341 gacaacaccg cccgtaaact acctgccagt tgattacgca cgggatcttt ttcctgcgcc
    11401 ggaaatgaat ctaccagtcg ctcaactgcc tgcgccgcac cacgcgtatg taatgttgcc
    11461 agcaccaaat gcccggtttc tgccgccgtc agcgccagac ggattgtctc gctgtcacgc
    11521 agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa tcccgatgcg
    11581 aacgtcatac aatgcaaacc gatttcccgc tgctggatca aacatcgctg gcttgcatag
    11641 agatattcga caggatcttc cagcgtcaga atatgcgcat cggcatgttg attgagatag
    11701 ccaaccatcg ccgccagcgt ggtagatttg ccgctccccg tcgcccccgt caccagaatc
    11761 aggccattct cgctcttgag taattccggc aataccggtg gcgcaccaag ctgttcgagc
    11821 tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg ttgcgcgaac
    11881 gcactgccac gcaaccgctg gttttccgcc agcgacacag caaaatccaa ctgaccattc
    11941 tccagcaata ttgtccgctg atcgtcatcc agccactccc gcagtagctc ttcgacgtcc
    12001 ggcgcatcaa acggcgcagc ttccattcgc ccgcgaatgc gccatcgtgc gggccaggcg
    12061 ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat ttcttccata
    12121 ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac aggtccggga
    12181 caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta cgctgcttgc
    12241 agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg ggcagcgtca
    12301 atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc aggaactggg
    12361 cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa gccgcctggt
    12421 ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta cccgcctcaa
    12481 cgatcagcgc ccggcagaac ttccccctct tgacgtactg attcaaatta atattagtga
    12541 tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag ctgcggtcgc
    12601 tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg agtcagaata
    12661 tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac tgaaaacgcg
    12721 ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag ccgccattgc
    12781 ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg attactctaa
    12841 aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc aacggtcatt
    12901 gagctgtata ccatggtgct gttattacgc atctggatgc agtgggctca ttgtgatttt
    12961 tacaacccct tctcacagtt tgtagtgaag gtaacgcagc caattatcgg gccactgcgc
    13021 cgcgttattc cggcaatggg acccattgac agcgcctcgc tgctggttgc ctatattctc
    13081 agttttatca aagccatcgt gctgtttaaa gtggtgacct tcctgccaat catctggatt
    13141 gccggtttac tgattctgct gaaaaccatc ggcctgctga ttttctgggt cctgctggtg
    13201 atggcgatta tgagctgggt aagccagggt cgtagtccga ttgaatacgt gctgattcag
    13261 cttgccgatc cgctgctgcg cccgattcgc cgcctgctac cggcaatggg tgggattgat
    13321 ttctcgccga tgatcctcgt tctgctgctg tatgtcatca atatgggtgt cgcagaagta
    13381 ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg agtgccgtaa
    13441 cagttaatga tgacggtctg gttttacggc tctatattca gccgaaagcc agccgtgatt
    13501 ctattgtcgg tttacatggc gacgaggtaa aagtcgccat taccgcgccg ccggttgacg
    13561 gccaggccaa cagccatctg gtgaagtttc tcggtaagca attccgggtt gccaaaagcc
    13621 aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc attaatccgc
    13681 aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca aaaagttgtc
    13741 ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag cgacttcggt
    13801 cttgatatcg tggcccaaac agacctcggc gtagattccg ctgaagaaac cggcctgacc
    13861 tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg tttaccggca
    13921 attgccgacg actccggtct ggcggtagat gcgctgggtg gcgcgccggg tatttactcc
    13981 gcgcgttatt ccggtgaaga cgcgaccgat caaaagaacc tgcaaaaact gctggaaaca
    14041 atgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct gatatatctg
    14101 cgtcacgcgg aagatccgac tccgctggtg tgccacggta gctggccggg cgtgattact
    14161 cgtgaaccag cgggtactgg tggctttggt tatgatccaa ttttcttcgt tccttctgaa
    14221 gggaaaaccg ctgccgaact gacccgcgaa gaaaagagcg ccatttccca ccgtggtcag
    14281 gcattgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc tgagtctcta
    14341 cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact ctcacgcgtt
    14401 gaaaggagaa gtgccgcacg acgattatgt tcagcatctg cttaacgatc tggacaacga
    14461 tgtggcttac gctcagggcc gtgaagtgca gacaatcttt attggcggtg gtacgccgag
    14521 cctgctttcc ggcccggcga tgcaaacgct gctggacggc gtgcgtgcgc gtttgccgct
    14581 ggcagcggat gcagaaatta cgatggaagc gaacccaggc acggtagaag cggatcgctt
    14641 tgtcgattat cagcgtgctg gtgtgaaccg catctctatt ggtgtgcaga gttttagcga
    14701 agaaaagctg aaacgactcg ggcgtattca tggcccgcaa gaagcgaaac gagcggcaaa
    14761 gctggcgagc ggtttagggt tacgtagttt taaccttgat ttgatgcatg ggctgccgga
    14821 tcaatcactg gaagaggcgc taggcgatct gcgccaggct attgaactga atccgccaca
    14881 tctttcctgg tatcaactga ccatcgaacc taatacgctg tttggctcgc gccctcctgt
    14941 actgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt tattaaccgc
    15001 agcgggctat cagcagtatg aaacatccgc ttacgccaaa cccggttatc agtgccagca
    15061 caatctcaac tactggcgat ttggcgacta catcggtatt ggctgcggcg cgcacggcaa
    15121 agtgaccttc ccggatgggc gcattctgcg taccaccaaa acgcgtcatc cgcgtggttt
    15181 tatgcaggga agatatctgg aaagccagcg tgatgtcgaa gctgcagata aaccgtttga
    15241 gttctttatg aatcgcttcc gtctgcttga ggccgcgccg cgcgtggagt ttagtcagta
    15301 tactggcctt tctgaagaag ttattcgctc tcagttagac gaggctatcg ctcagggtta
    15361 tctcacagaa tgtgcggatt actggcagat aaccgagcat ggtaaattat tcttaaactc
    15421 acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc attaatcact
    15481 tcttttaccc ccccccgt
//
LOCUS       DAZGHF010000111        15441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGHF010000111 DAZGHF010000000
VERSION     DAZGHF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.112"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            123..689
                     /gene="yceJ"
                     /locus_tag="ACHK3A_003666"
     CDS             123..689
                     /gene="yceJ"
                     /locus_tag="ACHK3A_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO8741700.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHQAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            693..1268
                     /gene="yceI"
                     /locus_tag="ACHK3A_003667"
     CDS             693..1268
                     /gene="yceI"
                     /locus_tag="ACHK3A_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO8741701.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1310..2362)
                     /gene="trhO"
                     /locus_tag="ACHK3A_003668"
     CDS             complement(1310..2362)
                     /gene="trhO"
                     /locus_tag="ACHK3A_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO8741702.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2587..3507
                     /gene="lpxL"
                     /locus_tag="ACHK3A_003669"
     CDS             2587..3507
                     /gene="lpxL"
                     /locus_tag="ACHK3A_003669"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO8741703.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKTLKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3679..4905
                     /gene="mdtG"
                     /locus_tag="ACHK3A_003670"
     CDS             3679..4905
                     /gene="mdtG"
                     /locus_tag="ACHK3A_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO8741704.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4988..5362
                     /gene="msyB"
                     /locus_tag="ACHK3A_003671"
     CDS             4988..5362
                     /gene="msyB"
                     /locus_tag="ACHK3A_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO8741705.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5363..5590)
                     /gene="yceK"
                     /locus_tag="ACHK3A_003672"
     CDS             complement(5363..5590)
                     /gene="yceK"
                     /locus_tag="ACHK3A_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO8741706.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5762..8305)
                     /gene="mdoH"
                     /locus_tag="ACHK3A_003673"
     CDS             complement(5762..8305)
                     /gene="mdoH"
                     /locus_tag="ACHK3A_003673"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO8741707.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8298..9833)
                     /gene="mdoG"
                     /locus_tag="ACHK3A_003674"
     CDS             complement(8298..9833)
                     /gene="mdoG"
                     /locus_tag="ACHK3A_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO8741708.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10227..11384
                     /gene="mdoC"
                     /locus_tag="ACHK3A_003675"
     CDS             10227..11384
                     /gene="mdoC"
                     /locus_tag="ACHK3A_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO8741709.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11392..12873)
                     /gene="clsC"
                     /locus_tag="ACHK3A_003676"
     CDS             complement(11392..12873)
                     /gene="clsC"
                     /locus_tag="ACHK3A_003676"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO8741710.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGIFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12815..13348)
                     /gene="ymdB"
                     /locus_tag="ACHK3A_003677"
     CDS             complement(12815..13348)
                     /gene="ymdB"
                     /locus_tag="ACHK3A_003677"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO8741711.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13443..13754)
                     /gene="ymdA"
                     /locus_tag="ACHK3A_003678"
     CDS             complement(13443..13754)
                     /gene="ymdA"
                     /locus_tag="ACHK3A_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO8741712.1"
                     /translation="MFRPFLDSLMLGSMFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13875..14207)
                     /gene="csgC"
                     /locus_tag="ACHK3A_003679"
     CDS             complement(13875..14207)
                     /gene="csgC"
                     /locus_tag="ACHK3A_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO8741713.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14266..14721)
                     /gene="csgA"
                     /locus_tag="ACHK3A_003680"
     CDS             complement(14266..14721)
                     /gene="csgA"
                     /locus_tag="ACHK3A_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO8741714.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14762..15217)
                     /gene="csgB"
                     /locus_tag="ACHK3A_003681"
     CDS             complement(14762..15217)
                     /gene="csgB"
                     /locus_tag="ACHK3A_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO8741715.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3846 a         3948 c         3665 g         3982 t
ORIGIN      
        1 tcattattaa ttcaaaatta ttgaaataaa aataaatatc attcaaattg ttatttttgt
       61 gttgcaattc attctccgac acgccatttt ctttctctcg caaagtcgtt ttggatgcac
      121 ttatgtcatt cacaaatacc cctgaacgct atggtgttat atctgcggcc ttccattggc
      181 taagcgcaat tattgtctac ggcatgtttg ctttaggtct gtggatggtc acgctcagtt
      241 attacgatgg ctggtatcac caggcacccg aactgcataa aagcatcggt attttgttaa
      301 tgatggggct ggttattcgc gttctgtggc gcgtcatatc tccaccgccg ggtccgctgc
      361 agagttattc gcccatgact cgtcttggcg caaaggctgg acatctcgca ttgtacttat
      421 tgctgttcgc catcggcatc agcggctatc tgatctcaac tgccgatggt aaaccgatca
      481 gcgtttttgg ctggtttgac gtccccgcga ccctcgccga cgctggcgca caggctgact
      541 ttgccggtgc cctgcatttt tggctcgcgt ggagcgtcgt agtactgtcc gttatgcacg
      601 gatttatggc cctgaagcat catttcatcg ataaagacga cactctgaag cgcatgctgg
      661 gaaagtcgtc atctgactat ggagtataaa aaatgaaaaa aagcctgctt ggtttaacct
      721 tcgcttccct gatgttctct gccggttcag cggttgccgc cgattacaaa attgacaaag
      781 aaggtcagca cgcctttgtt aacttccgca tacagcacct tggctatagc tggttatacg
      841 gcacctttaa agatttcgac ggtactttta cctttgatga aaaaaatcca gctgccgata
      901 aagtgaatgt gacaattaac accaccagcg tcgatactaa tcacgccgaa cgcgataaac
      961 atcttcgcag tgcagatttc ctcaatactg caaaatatcc acaggcaaca ttcacctcca
     1021 ccagcgtgaa gaaagacggt gacgaactgg atattaccgg cgatctgacg ctgaatggcg
     1081 taaccaaacc cgtcacgctg gaagcgaaat taattggtca gggcgacgac ccatggggtg
     1141 gtaaacgtgc aggcttcgag gccgaaggca aaattaagct caaggacttc aatatcaaga
     1201 cagatttagg tccagcttct caggaagtgg atctgattat ttcagtggaa ggcgtacagc
     1261 agaagtaaga gtgaaatgtt gccggatggc acgcatccgg caatgatatt tattctgttg
     1321 gatcaggaat gcccagtgtt gtattcagac gtccacgaga cttattaaag atcttattgc
     1381 cattttcacg tcctgcccga cggcgtcgct gttcttctgg cggtaacgcg ctttcttcgc
     1441 agcaaatttc actacaacaa cctttgtatt tttctgcaca tactggacac tgaataaaca
     1501 gcaggtggca gccatcattt ttacagttgg tatggctgtc gcacggcgca ccgcactggt
     1561 ggcaatgcgc gataatctca tcagatatac gttcgcccat ccgctcgtca aaaacaaaat
     1621 ttttgccaat aaaacgtacc ggtaaaccct gctcgcgcgc cttgcgggcg tattcaataa
     1681 ttccgccctc gatatgccag actttattga atccgttatg tttcatccag gcactggcct
     1741 tttcacaacg aatgccgccg gtgcagtaca taacgatttt tttatcttta tgtgcctgca
     1801 tcatctctac tgcttttggc agctgctcac ggaaggtatc tgccggaatt tccagcgcgt
     1861 tttcaaagtg ccccacttca tactcatagt ggttacgcat gtcgataaac aatgcatcgg
     1921 gatcgtcaag catggcgttc acttccgccg cttgcagata ctcacccaca ttgctggcat
     1981 caaagtgagg atcgtcaata ccgtcggcaa cgatacgatc gcggaccttc atgcgcagta
     2041 cccagaagga tttcccgtca tcatccaacg cgatattcag gcgtaagccc tctaaagccg
     2101 ggtcgaaggc ataaagctgc gcgcgaaatg tttcaacatt gctcgcaggt acgctgattt
     2161 gcgcgttaat gccctcatgc gccagataca ctcgcccaaa aacatccagc gcggtaaaca
     2221 gctgatataa agcgtcacgg gtcgccttag gatcggcgat gtggaaatac ttgtaaaacg
     2281 aaatggtggt tcgcggttcg ctctcagcca acattttggc ttttaacgcg tcgttggaaa
     2341 tgcggttgtg taacactggc atggtgtacg gttcctgcga gatgggaaag taaaaatccg
     2401 cggcatgata tagcaattat cgataattaa catccacaca ttttacgcta catttgcgca
     2461 ttaaaaatta tttgttattt acaagcgcgg caatttcgcc cagtcttcag ccacaaattt
     2521 tggttgcggg cgaaaaaatg cgacaataca tacaattgcc cgtataggtt gaaaaacagg
     2581 attgatatga cgaatctacc caagttctcc accgcactgc ttcatccgcg ttattggtta
     2641 acctggttgg gtattggcgt actttggtta gtcgtgcaat tgccctaccc ggttatctac
     2701 cgcctcggtt gtggattagg aaaacttgcg ttacgtttta tgaaacgacg cgcaaaaatt
     2761 gtgcatcgca acctggaact gtgcttcccg gaaatgagcg aacaagaacg ccgtaaaatg
     2821 gtggtgaaga atttcgaatc cgttggcatg ggcctgatgg aaaccggcat ggcgtggttc
     2881 tggccggacc gcagaatcgc ccgctggacg gaagtgatcg gcatggaaca cattcgtgac
     2941 gtgcaggcgc aaaaacgcgg catcctgtta gttggcatcc attttctgac actggagctt
     3001 ggtgcgcggc agtttggtat gcaggaaccg gggattggcg tttaccgccc gaacgataat
     3061 ccactgattg actggctaca aacctggggc cgtttgcgct caaataaatc gatgctcgac
     3121 cgcaaagatt taaaaggcat gattaaaacc ctgaaaaaag gcgaagtggt ctggtacgca
     3181 ccggatcatg attacggccc gcgctcaagc gtttttgtcc cgttgtttgc cgttgagcag
     3241 gctgcgacca cgaccggaac ctggatgctg gcacggatgt ccggcgcatg tctggtgccc
     3301 ttcgttccac gccgtaagcc agatggcaaa gggtatcagt tgattatgct gccgccagag
     3361 tgttctccgc cgctggatga tgccgaaact accgccgcgt ggatgaacaa agtggtcgaa
     3421 aaatgcatca tgatggcacc agagcagtat atgtggttac accgtcgctt taaaacacgt
     3481 ccggaaggcg ttccttcacg ctattaaatc tcccaagccg gatgcttgag aatagcatcc
     3541 ggcattacca cagcaaatcc ccctgattta gcgataaaag ctctctggat tgcgccccct
     3601 ggaagtcggg cgcataatta gtgtgcttat cttttcttct tatgttcacc gcgcctggcg
     3661 caccaacagc ggattgctat gtcaccctgt gaaaatgaca cccctataaa ctggaaacga
     3721 aacctgatcg tcgcctggct aggctgtttt cttacgggtg ccgccttcag cctggtaatg
     3781 cccttcttac ccctctacgt tgagcagctt ggcgttacgg gccactccgc cttgaatatg
     3841 tggtccggta ttgtcttcag cattacattt ttattttcgg ccatcgcctc accgttttgg
     3901 ggtggactcg ccgaccgtaa aggccgaaaa ctcatgctat tacgctctgc cctcggcatg
     3961 ggcatcgtga tggtgttgat gggactggca caaaatatct ggcagttttt gatcctgcgg
     4021 gcgcttcttg ggttacttgg cggatttgtc cccaacgcta atgctcttat cgccacacaa
     4081 gtaccgcgta ataaaagcgg ctgggcgctg ggtacgctct ccacaggcgg cgttagtggt
     4141 gcgttgctcg gcccaatggc tggcggcctg ctcgccgata gctacggctt acgtccggta
     4201 ttctttatta ccgccagtgt gctcatactc tgctttttcg tcaccctgtt ctgcatcaga
     4261 gaaaaattcc agccggtcag caaaaaagag atgctgcaca tgcgggaagt ggtgacatca
     4321 cttaaaaacc cgaaactggt actcagcctg tttgtcacca cgttaatcat ccaggtggcg
     4381 acaggctcaa ttgcccccat tctgacgctg tatgtccgcg aactggcggg taacgtcagt
     4441 aacgtcgcct ttatcagtgg catgatcgcc tcggtgccag gcgtggcggc tctgctaagc
     4501 gcaccacgac tcggcaaact tggcgatcga atcggacccg aaaagatcct gattacagcg
     4561 ctgatctttt ctgtactgct gttgatccca atgtcttacg ttcagacgcc attgcaactt
     4621 gggattttac gttttttgct cggtgccgcc gatggtgcac tactccccgc cgtacagaca
     4681 ctgttggttt acaactcgag caaccagatc gccgggcgta tcttcagcta taaccaatcg
     4741 tttcgtgata ttggcaacgt taccggacca ttgatgggag cagcgatttc agcgaactac
     4801 ggtttcagag cggtatttct cgtcaccgct ggcgtcgtgt tattcaacgc agtctattca
     4861 tggaacagtc tacgtcgtcg tcgaataccc caggtatcga actgattttt cgcctttcat
     4921 acttgcaaaa gcggagaatc agctatcctt ttccctgaaa cctcatcaac tcaaagggag
     4981 aatcgtgatg accatgtacg caacgcttga agaagccatt gacgctgcac gcgaagaatt
     5041 tcttgcagac aaccccggca tcgacgccga agatgcgaat gtgcaacagt tcaatgccca
     5101 aaaatacgtt ttgcaggacg gcgacatcat gtggcaagtt gagttttttg ccgacgaagg
     5161 ggaagaaggt gaatgtttac ctatgcttag cggtgaagcc gcgcaaagtg tttttgatgg
     5221 cgactatgat gagatagaga tacgccagga gtggcaggaa gagaatacat tacatgaatg
     5281 ggacgagggg gaatttcagc ttgagccacc gctggatacc gaggaaggac gcgcagcagc
     5341 tgatgagtgg gatgaacgtt aatcactcat acgggccatg atgaatgtcg atcggcagca
     5401 gtaaagtatc gaaaaccagg gagaatggca gatcaaggat cgtgacgtaa cgccaggcgg
     5461 agtcacgcac gtcccattgc acaccgggat aatattggtt gccatgcccc tgccccggta
     5521 tagtgcgact aatgatgctg ccacagccac tcagcaaggt caccattatg ctcatcacaa
     5581 ttaaacgcat ttgatacctc ttccgtaaaa aataccggca taacgccggt atttttattg
     5641 gctcgttgtt tttgccggat gcggcgtgaa cgctttaccc gacctacaaa atcttgcaaa
     5701 atcaataaat tgcaggaacg atgtaggcct gataagcgta gcgcatcagg caactacgtt
     5761 tttattgcga agccgcatcc ggtttacgca atgccagtgg attgagcttt atcccttcgt
     5821 aataactcac ccatgaagaa tatctctccg gggaattcca gacgcggaaa tgcagacggg
     5881 ccatcgtcac cggatcgctt agcagcacca ggcgacgatc gcgattcagc ttctctggcg
     5941 tctcgttcag cgcctgttca acgtggcggt cacgggcgat ttccagcacc ttgctggcgc
     6001 ggtgacgcgc ggtcgccatt gcggttgcca gagcgttaaa tgacgggtta aacaccgcgt
     6061 gcataaaacc atcatcaagg gagcgttgac gattcatctc aaggaaccga tcggtatcaa
     6121 ccagcacctg cggcggtgaa tactcttccg ggatcaggaa cagtttccag cgtttggttc
     6181 gcagaccaac ggtggcacgg ctggaaatca ccgaaacaaa cggtgacagg atcaacgaga
     6241 agacaatcgg tgccagccag aacagaaaac gcagatccaa ccacgccatc ccaacagccc
     6301 acactaaacc caacagcagc tgtgagccgt ggcgtttgaa tgcttcgccc caggaagtgg
     6361 agtcatcatc acgctgcggt gaattccaca ccacttccca gccaaggaac gcgctgacga
     6421 cgaagactgt atggaacaac atgcgtaccg gagccagcaa cacggaaaac agcacttcca
     6481 gcagcaacga taatgtaacg cgccagaagc cgccgtattc tttcgttcct ttgcaccaga
     6541 taagcaaaat gctcaatagc ttcggcagga acaacagcac catggtcgaa gcaaaaagtg
     6601 caatcgccag ctcaggacgc cactgcggcc ataccgggaa caactgccgt ggttgcagga
     6661 agtattgcgg ttcggtcaac gcatgtacta cctgcaatgc agtagagagc gcgaggaaca
     6721 taaaccacag cggagcggag agataagaca tcacgcccgt caggaacacc gcacggtgaa
     6781 ccgggtgcat acccttcacc aggaacagac ggaagttcat caggttaccg tggcaccagc
     6841 ggcggtcacg ttttagctca tcaagcaagt taggcggcaa ttcttcataa gaacccggga
     6901 gatcgtaagc aatccacacc ccccaacctg cacggcgcat caacgccgct tctacgaagt
     6961 catgtgacag gattgaaccg gcaaaagaac cttcgcccgg cagcggagcc agtgcacaat
     7021 gctcgataaa cggtttcacg cggataatcg cgttatgccc ccagtagtgc gactcgccaa
     7081 gttgccagaa gtgcaaaccg gctgtaaaca gtggcccata cacgcgggtc gcgaactgct
     7141 gacagcgcgc atacagcgta tccataccgg acgctttcgg cgacgactga atgatcccgg
     7201 cgttcgggtt ggcttccatc aagcgcacca ggccgcataa acaatcaccg gtcattaccg
     7261 agtcagcatc cagcaccacc atgtagctgt actggctgcc ccagcgacgg cagaagtcat
     7321 cgatattacc gcttttacgc ttcacgcgac ggcggcggcg gcgatagaaa atctgacctt
     7381 cgccaccgac ttcagcgata agctccatcc aggctttttg ctctgcgacg cagatatccg
     7441 ggttataact gtcactcaga atgtagacat caaagtgttt ggcattcccg gtggctttta
     7501 ctgattccca cgttgcacgc aagccagcaa aaacacggtt cacgtcttcg ttacagatag
     7561 gcatgatcaa cgccgtgcga tgctccgggt ttaatggttc atcgccaact gttgacgcgg
     7621 atatactgta tttatcgcga ccaataagca gttgcaggaa gcccatcaac gccgtccaga
     7681 atccggcgga cacccaacag aacagtaccg caaagaggat caggataccg gtttgcagca
     7741 tataaggcag aagctgcata aaagaaaccc acacatcctg accaaccata tccataggat
     7801 taatcagcgc ccacccctga taaggaagaa tggtcttcat ataccaggtc gcgacaacag
     7861 tttgcgcgag cgtcaggatc aacagaatgt aacggcggat ggtaccgacg gtacgccact
     7921 tttgctcgct ctcctgctct tctttggtca aacgagccag atagcgcggc gtcacatcgc
     7981 gtccacgcag gcgatcccag aaacggccta ccgggttggt acgccacggg tcgggaaaca
     8041 tcgaggagcg ttttgcttct ggcatcgcct taagctgatc gcgcccttcg tcatctttaa
     8101 ttaactgtcc atcagcaagt gaatctggcc aggcttgttc cagacgcgcc tttaccgagc
     8161 cttgcgggga gtcatcctcc cgcgcccagg tgcggtgttc ggcatccagc gcctgatgaa
     8221 cggcgcggat atcagtcttc ggcaatgccg ctttctcgct tgcggcgatg ggcattgcgt
     8281 caatgtactc agttgtctta ttcattggca ggtaactggt agctccaggt ttcactcaac
     8341 gtctgatcgg cattcaccag cgcagcacgc atttcagtgg ttttcttggc atctttcact
     8401 ttcacacgca tcaccagacg ccagcctttg gtaaccgggt tatagcgcac cgtgctttca
     8461 actatctcac cattatcacc aatgctggtt tgcgctgtga ccggggtatc ctctggcagt
     8521 tttttcatct ctgcgccggt aaaatcgacc acaaaggcga tagtaccgtc aggttggcga
     8581 atcaggttcg actgcttcac atcccccgtt gaacgacgcg tttgttgcac ccatgcgtta
     8641 tctggcgcat gcagtttgtc ttcatcacgg ctgaaggtga tggtgtattt aaagttcatc
     8701 tctttacccg gctccggcag ctgatccggc gtccagtaag cgacgatgtt atcgttggtt
     8761 tcatcgttgg ttggaatttc caccagctca acgctgcctt tgccccactc ccctttcgga
     8821 gtcacccatg cgcttggacg gagatcgtaa cgatcatcga gatcttcaaa gcgggagaaa
     8881 tcacgaccgc gctgcaacag accgaagcct tgcgggtttt ccatcgagaa gctgctgacc
     8941 gctaaatgtt tcgggttatt caacggacgc cagatccatt cgccattacc agcatggata
     9001 gagagaccgt tagagtcgtg caactccgga cgatagttat ttgcaggcga cggttggttc
     9061 ggcccaaaca ggaacatact ggttaacggt gcaaccccca gtttgccgac tttatcgcgc
     9121 agatagattt tcgactgcac atccacaacc gtgtcacgcc ctggcataac tacgaatttg
     9181 taagcacctg tcgcgcgcgg cgagtcaagc aatgcataaa tggttaaacg tttatcagtc
     9241 ggttttggac gctcgatcca gaactctttg aagcgtggaa attcttcacc cgatggcaag
     9301 gcggtatcaa ttgccaggcc gcgtgcagaa aggccataaa cctgacctgc accaatcacg
     9361 cggaaatagc tggccccgag catgctgacg atttcatcgt ttttatcttt gctgttgatc
     9421 gggtaaagca ctttaaaacc ggcaaaacca aggtctttta ccgtgtcttt gtcatgctga
     9481 acatcgccga aagtgaaata atccgggctg tatttgattc gtttgactgc ggtggcagtc
     9541 acttcattta ttttgaccgg ggtatcgaag tacataccct gatggtagaa ctcgagtttg
     9601 aatggggtct tcagattgtt ccagtacgct ttgtcatgat taaactggat ctgctgatag
     9661 tccgcgtatt tcatgtcgcg gaaaacggag ggcaagttgc ttttaggcgc ctcatagcct
     9721 ttcccggcta aggattgagc ttgctttgcg acatcatcaa tactgaaagc ccagcttgaa
     9781 gatgtataca gggttaacat tactgcagca ctcaaccaac gcattttcat catttgtagt
     9841 ttatgtttca taattagtaa gcacttcccc ctttgtgtgc ttatatcgat ccgatctatt
     9901 ttaatggata accagccatt ccgacaacag aatcccggta tcgttcagca cgctggctca
     9961 tggattaagc acctgattgc aaccgttttc actttccgtg cttctcctaa agacagaagc
    10021 atcgacttcg tgtagggttt gccagcaaat ttatttatca accgtcttac agacgcttcc
    10081 attctggtta gtggtagcga tgatgctatg cataaaaacc ttgaagcctg cgcctggcgc
    10141 ggtcaggtcg gtgtcgctat ggcaacaagc ctaaaggcat aacccgacag ggataagacg
    10201 aaaaatccga gattagttaa ccatatatga atccagtacc cgcgcaacgt gaatatttcc
    10261 tcgactccat ccgcgcctgg ctgatgttgt tagggatccc ttttcatatt tctttaatct
    10321 attcgagcca tacatggcat gtgaatagcg ccgaaccgtc attgtggctg acccttttta
    10381 atgacttcat ccactcgttc cgcatgcagg tatttttcgt tatatccggg tacttttcct
    10441 acatgctttt tttacgctat cccttgaaaa aatggtggaa agtacgtgtc gaacgtgtag
    10501 gtatcccgat gttaacagcc atccccctac tgacattacc gcaatttatt atgctgcaat
    10561 acgtcaaagg gaaagcggaa agttggcctg ggctatcatt gtatgacaaa tataatacgt
    10621 tggcctggga attaatatca cacctgtggt ttttactggt gttagtagtc atgacgacgc
    10681 tgtgcgtatg gatatttaaa cgcatcagaa ataatttaga aaattctgat aaaacgaata
    10741 aaaaattctc gatggtaaaa ctatcggtga tttttttgtg cctcggcatc ggttatgcgg
    10801 taataagaag aacgattttt attgtgtatc cgcccattct gagtaatggc atgttcaatt
    10861 ttattgtcat gcaaacgcta ttttatttgc cgttctttat cctcggcgca ctggctttca
    10921 ttttccctca tcttaaagcc ttgtttacca cgccgtctcg tggctgtacc cttgcagcag
    10981 cattggcatt tgtcgcttat ttactcaacc agcgctatgg cagtggcgat gcctggatgt
    11041 acgaaaccga gtcggtgatc accatggtcc tcggtctgtg gatggtgaat gtggtcttct
    11101 cctttggcca ccgtttgctt aacttccagt cagcgcgggt gacttatttt gttaacgcat
    11161 cgctgtttat ctatctggtt caccacccgt taacgctgtt tttcggcgca tacattacac
    11221 cgcacatcac ctccaactgg cttggttttc tctgtggcct gatattcgta gtagggattg
    11281 cgataattct gtatgaaatt catttacgca tcccgttact gaagtttttg ttctctggta
    11341 aaccggttgt caagcgtgag aacgataaag caccagcccg ttaagccacg cttataataa
    11401 ccattccacg ggcaatatcg acgccagtct gaccataacc cgcttccaga aactggtggc
    11461 gggttctttt ttgagaataa tctctttctt tgcatgacga tcaacccagt tgatccgtcc
    11521 ccacctgtcc agacggagct gccaggccgc atcatactgg ctctgaataa agcgtttatc
    11581 aattaactgt gccagcgttt cgctctctat cacgaagccc atttcagtat tgagcaatgt
    11641 tgaacgcgga tcgaaattga aagagccgat aaacaccgtt ttaccatcga tgctaaaggt
    11701 tttagcatgc aagctggcac cggaattacc ggttatgccg cgatcgtgta acgtactact
    11761 ttgttcacgc gtcggcttga gttcatataa ttccacgcca tagcggagca attttttgcg
    11821 ccagcgcgcg tatccggcat ggacgacagc aacatcgtta gcggcaagag aattggttag
    11881 gatcgcaatc tttacccctt ttctcaccat ccgtaagagt tgcgccacac ctgcgcgtgt
    11941 cggtacaaaa taggaagaga taatatcgat gcgttcactg ggtgagccca tgatatcgaa
    12001 caggcgctgc ggtaacagtg aatgccgttt tgccttgcct tcccctttcg ccggatcatc
    12061 acttaataaa cgtgttttcg cccagataag cggcaacgtt ccatcaacca gatgatttat
    12121 aaacgggctg gattccattt tgcgtaaata acgatgcgtc atggcatcgt tgtgccagga
    12181 tgctggtaac tcgatgcgat ccgccatttc accttccggg acatccagca cctgctgtag
    12241 gggtgaaacc gatttgcaat accagtagcg ggcgaaatca tcggcaacgt cctctacaac
    12301 tggtcctatt gccatgacat ctaaatccga aaaaagtggc tcttcccctg ccccaaaata
    12361 ggcatcacca atatttcgcc cccctaccag cgtcaccacg ccatcgacag tgaaactttt
    12421 attgtgcata cggcgattga gacgggaaaa gtcggtgata taaccaagcg gacgtagcaa
    12481 gcgaaacgag aaaggattaa aaagccggac ttcaatgcgg ggatgactgt caagcaagcg
    12541 taaaatgtcg tcaagtccgg gcgtattgtt gtcgtccagc aacaaacgga cgcgaacgcc
    12601 acgctttgct gcggctaaca gggcggaaaa gagtaaccga cccgacatat cgtcctgcca
    12661 gatgtaatac tgaacatcca gcgtatgctc tgccatttcg gccagacgat accgggcggc
    12721 aaacgcatcc agacttttct ccagaggaaa aatgccacac tgaccgggat gttgcgaaca
    12781 cagtggcagc accgcactcg ccagccgggg caaatcattc atctccttgt tgagtaagga
    12841 gtctttcgta gaggtgggcg ttttcttcat cataacagac aaagtatacc tgttcgggta
    12901 aagcgtgacg ggtaataaat tctgaaacgg tttttactgc gatttcagcc gctgccgcac
    12961 gagggtaacc ataaactcca gtactgattg caggaaaagc cactgacgta tagctgtttg
    13021 ctgccaccag tcgcaggcta ttgagatagg catcctgcaa aagctggtct tcgttttgtt
    13081 caccaccacg ccagactggc ccgacggtgt gcactacggc tttcgcggga agatcgcctg
    13141 caagcgtaat aacggcatgc cccgtagggc aatcgccctg ctgctgcctg acttttaaac
    13201 aggcatccag cagggccgga cccgctgcgc gatgaatggc cccatcgacg ccgccgcctc
    13261 ccattaatga cggattagcc gcattcacaa tcacatcaac ggccagtttg gtaatatcac
    13321 cctgcacaac atgaatacgc gttttcataa cgcctcctta caccttcttg tttcttaagt
    13381 gtacagcagg ctttttggtt aaatcgcatt tttctgctca gcaacgtgag ttgttcagaa
    13441 acttagttat attcgatgtt cattacagcc agctttttct gtttatcaag atactgcact
    13501 tttactgagg ctatttgcgc gtttttgaca ttgccgctca ttaacgcttt gctggaatag
    13561 gttctgcccg gaacagagcc attctgtggg cagttcattt ctacgggtga tgactgggtg
    13621 ctgacgtcac atgccggttc cacaatttgg ccataaaaat ggatcacgcc cccttgcgcg
    13681 gtgcttcctg cgatggcaat aaaaggaaaa aacatactgc cgagcattag agagtctaaa
    13741 aaaggtctga acatttattc tatttcctca atgattagtc atccttgagg gttgtgttat
    13801 ccatactttc aggttggctg atccgcagca gaccattctc tccagattca tcttatgctc
    13861 gatatttcaa caaattaaga cttttctgaa gagggcggcc attgttgtga taaatgaagt
    13921 gactgtccat cagaaacagt aacaactatt ttcacccgat cgtccgggga aatatttaaa
    13981 ctcaacttcg tcaaagcaat gggttgatta gcaggcaatg agagggtctt ttcttgcttc
    14041 gtctgacttt gccctgaact gccttcgcgc agggacaata tttgtactct gcacagacaa
    14101 gattgagtaa gagtgacttc aggaataatg gtgtacatat ccccttgctg ggtcgtatta
    14161 aaggttatct gactggaaag tgccgcaagg agtaataacg cattcatatt cttctcccga
    14221 aaaaaaacag ggcttgcgcc ctgtttcttt aatacagagg atgtattagt actgatgagc
    14281 ggtcgcgttg ttaccaaagc caacctgagt gacgttgacg ctggagttag atgcagtctg
    14341 gtcaacagca gcaccgttac caccaccgaa ctgtttaaca gtcatagtag aatctttacc
    14401 attccactga tcaagagtag cgctgttgcc gaaaccacgc tgagtcagat cgattgagct
    14461 gtcatcagaa ccttggccaa catctgcgcc gttaccgccg ccgtgctggg taatggtcag
    14521 atcagagtta cgggcgtcag cttgcagagc aagagcagag ttaccgccac cgtactggta
    14581 aatgttcagc tctgaatttg ggccgctatt attaccgcca ccaccgtggt taccgccgcc
    14641 gccgtactga ggaacaacac ctgccagagc gctaccagag aatacgattg ctgcaattgc
    14701 tgctactttt aaaagtttca tgtaaaaccc ccatcggatt gatttaaaag tcgaatggaa
    14761 attaacgttg tgtcacgcga atagccattt gcgactgtct ctgcactaca attgccgttt
    14821 tttgagtacc atactgtgta atatttgctt tattaccaga acctttctgg ataatcatcg
    14881 cagtattacc ataagcacct tgcgaaatac tggcatcgtt ggcactgccc gcctgatcaa
    14941 tatatgcaag gttataatct cctgtctggt caatctttgc ccggttgcta ctaccttctt
    15001 gcgcaacaac cgccaaaagt tttgagcctc cctgccgtaa ctgagcacta ttattagtcc
    15061 cagcttgacc aattatggct gcctgattaa atgaagactt actcaattca tttaccgcga
    15121 agttatattc tgaattagct aaatcataac ctgctgcggc tgcaatccca ggcgcaccca
    15181 gtattgttaa catcataaat aacaatttgt ttttcatgtt gtcaccctgg acctggtcgt
    15241 acatttaaga aattaaatca tttcaacttg gttgttaacg caacctgtat tttgttaacg
    15301 ctgcgttacg atggaaagta tgtctgcgga aatattttta ataactcacc cgcgcgttgt
    15361 atttttcttt ttttgttcat accaaagtat taaaactcac gtaaatacgc tgttatctac
    15421 gcaaaaaata ttttgttttc t
//
LOCUS       DAZGHF010000112        15362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGHF010000112 DAZGHF010000000
VERSION     DAZGHF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.113"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1832)
                     /gene="flhA"
                     /locus_tag="ACHK3A_003682"
     CDS             complement(<1..1832)
                     /gene="flhA"
                     /locus_tag="ACHK3A_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO8741716.1"
                     /translation="MSNLAAMLRLPANLKSTHWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLL"
     gene            complement(1825..2973)
                     /gene="flhB"
                     /locus_tag="ACHK3A_003683"
     CDS             complement(1825..2973)
                     /gene="flhB"
                     /locus_tag="ACHK3A_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO8741717.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKSILVGSVTG
                     FYLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(3162..3806)
                     /gene="cheZ"
                     /locus_tag="ACHK3A_003684"
     CDS             complement(3162..3806)
                     /gene="cheZ"
                     /locus_tag="ACHK3A_003684"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO8741718.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(3817..4206)
                     /gene="cheY"
                     /locus_tag="ACHK3A_003685"
     CDS             complement(3817..4206)
                     /gene="cheY"
                     /locus_tag="ACHK3A_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO8741719.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(4221..5270)
                     /gene="cheB"
                     /locus_tag="ACHK3A_003686"
     CDS             complement(4221..5270)
                     /gene="cheB"
                     /locus_tag="ACHK3A_003686"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO8741720.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(5273..6133)
                     /gene="cheR"
                     /locus_tag="ACHK3A_003687"
     CDS             complement(5273..6133)
                     /gene="cheR"
                     /locus_tag="ACHK3A_003687"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO8741721.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNLHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(6152..7753)
                     /gene="tap"
                     /locus_tag="ACHK3A_003688"
     CDS             complement(6152..7753)
                     /gene="tap"
                     /locus_tag="ACHK3A_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO8741722.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLESASAQSQRNYHISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(7799..9460)
                     /gene="tar"
                     /locus_tag="ACHK3A_003689"
     CDS             complement(7799..9460)
                     /gene="tar"
                     /locus_tag="ACHK3A_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO8741723.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(9605..10108)
                     /gene="cheW"
                     /locus_tag="ACHK3A_003690"
     CDS             complement(9605..10108)
                     /gene="cheW"
                     /locus_tag="ACHK3A_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO8741724.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(10129..12093)
                     /gene="cheA"
                     /locus_tag="ACHK3A_003691"
     CDS             complement(10129..12093)
                     /gene="cheA"
                     /locus_tag="ACHK3A_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO8741725.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRDQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISPPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(12098..13024)
                     /gene="motB"
                     /locus_tag="ACHK3A_003692"
     CDS             complement(12098..13024)
                     /gene="motB"
                     /locus_tag="ACHK3A_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO8741726.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(13021..13908)
                     /gene="motA"
                     /locus_tag="ACHK3A_003693"
     CDS             complement(13021..13908)
                     /gene="motA"
                     /locus_tag="ACHK3A_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO8741727.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(14035..14613)
                     /gene="flhC"
                     /locus_tag="ACHK3A_003694"
     CDS             complement(14035..14613)
                     /gene="flhC"
                     /locus_tag="ACHK3A_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO8741728.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(14616..14966)
                     /gene="flhD"
                     /locus_tag="ACHK3A_003695"
     CDS             complement(14616..14966)
                     /gene="flhD"
                     /locus_tag="ACHK3A_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO8741729.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         3585 a         4328 c         3746 g         3703 t
ORIGIN      
        1 agcaacaaac gttccagcgg tgtatcgagg ccaataacat ggacttcatc tttgccagga
       61 aaccactgct gggtaatcgc ccggcccaac gctacgcgca cgacggcggt taattcatgc
      121 ggatcgcttt ggatgggcgc atgttccgcc agcgtttcga gaatggtgcg catatcgcga
      181 atcggcactt tttcatcgag cagattttgc agcactttat gcagtgtggt aagcgtgacg
      241 acaccaggaa cgagatcttc cgtcagcttt ggcatctcct gggcgacgcg atccagcagc
      301 tgttgtgcct cctgacgacc aaacagctct gcggcatgct ggctaatgag gtggttaaga
      361 tgggttgcca ccaccgtgct ggcctcaacc acggtgtacc cctgaatctg cgcctgttct
      421 ttaagcgcac tttcaatcca gatagcattc aggccaaatg ccggatcgac ggtcgcctca
      481 ccaggtaacg tcccggcagc agtaccaggg ttaatcgcca accaacgccc cggataagca
      541 tcaccactgc ctatctccac gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc
      601 atattgtcgc gaatgtgcac caccggcggc agaaacccca tctcctgggc aaatttcttg
      661 cgaatactgc gtatacggcc cagcaactca ccatcctgct ggaaatcgac catcgggatc
      721 aggcgataac ccacttccat tcccagagaa tcttccagtt gcacatcgtt ccacgtcgct
      781 tcgacaacgg tattattctc tgccattttt accggttttg gttcggcagg cgctttttgt
      841 tcacgtccgc ggatccacca ggccagtccc agcaatccgg cagtgaacag caaaaatacc
      901 aggttcggca ttccaggcac caggccgagt aaaccgagca cggcggcgct taacaacata
      961 acgcttgggt tactgaaaag ctgattcacc atctgctcgc caacatcctg atcggtgctg
     1021 acacgcgtaa cgatgacccc cgcggcggta gaaatcacca acgccggaat ttgtgccacc
     1081 agaccgtcgc caatagtcaa tagcgtataa ctttccgccg cgtgtcccat gctcatacca
     1141 tgttgcagca cgccgaccag caacccaccg acaacgttta tgaccatgat gaggatcccg
     1201 gcgatggcat cgccgcgaac aaacttactt gccccgtcca tcgagccgta aaaatcggct
     1261 tcctgagtca cttcggagcg gcgttttttc gcttcatctt caccaatcaa tccggcgtta
     1321 aggtcggcgt caatcgccat ctgcttcccc ggcataccat cgagaacaaa gcgcgcgcct
     1381 acttccgcaa tacgcccggc acctttggta atgaccataa agttgatgat cacgagaatg
     1441 acgaacacca cgataccgat agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg
     1501 accacctttc cagccgccgc cgcgccggta tgcccttcca tcaaaatgat acgggttgaa
     1561 gccacattaa gtgccagacg caacaacgtg gtaaacaaca gaatcgtcgg aaacgcagca
     1621 aactcaagcg tgcgttgggt aaacatcgcc accagcaaca ccatgatcga caaggcaata
     1681 ttgaaggtaa acagcaggtc gagtatgaat gcgggcagtg gcagtaccat catcgacaag
     1741 atcaacagga tcaaaatcgg tccggcaagg atctgccagt gtgtcgattt caggtttgcg
     1801 ggcaggcgca gcatcgcggc cagattactc atgggtcggt ttctcgttaa taaaatccag
     1861 ggcttccggc accggaagat gagtaggttg tacagggcgc tgtccaccag ccagacgcca
     1921 gcgtttcagt tgccagaccc aggccagcac ttccgccacc gcggcgtaca gttgacccgg
     1981 gatttgttga ccgatctccg catgtcgata cagcgcacgc gccagcggcg gcgcttcaag
     2041 cgtcgggacg ttattttcag cgccaatttc acgaatgcgc agcgcgacca gccctgcacc
     2101 tttagcgacc actttcggtg cgctcatttt gttttcgtca tactgcaaag ccactgagta
     2161 gtgagttggg ttattgacga taacgtccgc tttcggcaca tcggccatca tccgccgccg
     2221 cgccgcagct cgctgcatct gacggattcg ccctttgaca tgagggtcgc cttcgctttg
     2281 tttgaactca tcacgaatat cctgccgtga catacgcagc tttttcaggt ggctgaagat
     2341 ttggaaaaag acgtcaaatc ccaccatcgg aatgacacca agcaccacca gcaatgcgca
     2401 cagccctacc agatccatcg cattacccat ggcggtaatg ggggactccg ccattaagcg
     2461 catcatttgc ggccagtggt gccagagata aaaccccgtc acactgccga ccaggatgga
     2521 tttcaaaatc gctttaagca actccgcacc ggtttgtgcc gaaaacatcc gtttaatgcc
     2581 cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa ataccaggcc
     2641 acccaacata accggagaaa taagcgccac cagcaccaca ccgctaatca gcggcagcag
     2701 cgccagcatg gcttctctga tcagcagaat aatttgcccg aggatcagat tcgggtcatt
     2761 gataatactg tgatcaaaat gcagcccagc ggagagcatg cccgacaatc gacgggccag
     2821 cgacacaccg ccaaaccaga taacacacac gcccactaac aaaatcagca gtgacgttaa
     2881 ttcacgtgaa cgcgggattt gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg
     2941 ggcttctgtt ttgtcgtcgc tctcgtcaga cacgtcgcca atcctggaaa gagtattaag
     3001 cgtaaatgat gccagagcgc aaagcgttca atggtttgag taaggggcaa aacaggcggg
     3061 atttagggct tttactgcca cacatcaagc atagtatgcg tttgtcggat gcggcgtcat
     3121 cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc aagactatcc
     3181 aacaaatcgt ccacctgatc ctgactggct accacaccgg ctttgctggt atcgacctga
     3241 ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc cgggatgttt
     3301 tccaacagca ccatcaacaa ctggcgttcg atctcctgaa tgacatccat catccgctta
     3361 ataacctgcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag cagttgcgcg
     3421 ttagtaaagc tggtatgcgc ggggacatct gccagaaatt gccgcgtatc tgttaccagt
     3481 tctcgggcgt cggcaaggtc aatcggatcg gcaaaccagt catcccaacg ttgggttaac
     3541 gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac actgttcagc
     3601 gcccgctccg cagcctgggc agtcatctgc acaacatagt acaaacgatc gcgcgcatcg
     3661 gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg caaactgtcg
     3721 cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga atgctcgtca
     3781 gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt tctcaaagat
     3841 tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat agccactggc
     3901 ccccgcttgc gccgcagcaa taatgttctc tttcttcgct tctgcagtca ccattaacac
     3961 tggcaatgct gacatcgcgc catccgcacg aatcgttttc agcaattcca ggccatccat
     4021 gttgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca acttattgag
     4081 agcgtcgaca ccatcttccg cttcctcaac attattgaat cctagctctt tcagcaggtt
     4141 acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa gttctttatc
     4201 cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat ttttgccagc
     4261 atttgctggc ttacctggct aagatcgacc acttcgcaaa caccacccat attgatggcc
     4321 tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gtgcaagggt ccatgccccc
     4381 gcctgacgca tcgccaacat tcccaccgcg ccgtcgttgc ccataccggt caggatcacc
     4441 ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc taccgaaggc
     4501 cgatgacggt taaccgccgg gccatcgtga attttgattt ggtaatttgc gccactacgc
     4561 gccagctcca tatgccgatc gcccggcgca atataagcat gccccggcaa gacacgttct
     4621 ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc ggcaaaagag
     4681 cgggtgaaac cgggcggcat atgctgggta attaacagtg ccgggctgga aagcggcaac
     4741 ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt
     4801 ttttcagagc tcaacaacgg ccctgccttc agcgttgtcg gtgccgacaa tggcttatgt
     4861 gctgcaaggc tcgcctttgc tgctgtacgc accttttcag caatcatttc gctatacgcc
     4921 agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctatcgc ccccagctcc
     4981 aatgcgcgca gcgtgacttc tgatcctttg ccggtcaggg aagaaaccat cacaacgggc
     5041 attgggcgca aacgcattaa tttttcgaga aaatccagtc cgtccatccg cggcatttca
     5101 acatccagcg ttagcacatc cggattgaat ttcttaatca aatcacgcgc aaccagcgga
     5161 tcaggggcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc
     5221 tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat cgttaatcct
     5281 tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg tggctaaagt
     5341 tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca aagcggcgca
     5401 aaatctcctg ctgggtagtt tgatcgaagt agatcatgac gttacgacag aagatcgcat
     5461 caaacggccc cggcacggtg tactgtttcg ccagtaaatt cagcggggca aaatcaacat
     5521 agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc cctcgcatga
     5581 aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga taaataccgc
     5641 ttctggcttt ttccagcact tcggtgtcga tatcgctggc aaacactttc cagcgtccgg
     5701 gcacggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct tcgccggtcg
     5761 aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga tccgcgagca
     5821 gaggaaaatg atgcgcctca cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa
     5881 acgcctgcca ctcaccgctg tgcagattag attccagcaa gttcaggtaa tgaccgaaat
     5941 ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat
     6001 ggtcggccag gacgatcccg gctcgttgat agatcaattg acttatccgc cgaaaatgcg
     6061 cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc ccacagggca
     6121 gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca atttgtaact
     6181 gcgccgactc atgtcgtgct acttcatgct cttcaagggt aaataccgcc acgcgcgacg
     6241 aaagatggtc ggcctgattc gccagttgtt ctgttgccac cgccgcttct tctaccaggg
     6301 aggcgttctg ctgtgtcacc tgatccatct ggctgacagc ctgtgcaacc tgctcaatcc
     6361 cccgccgttg ttcttccgag gccgaagcaa tttctcccat aatgtcgttc acgcgggtca
     6421 ccgaactgac aatatcagtc atggtcgcgg cggcgttatt caccagtttc gaaccctgct
     6481 ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc tgggcgctac
     6541 ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc tgctctccgg
     6601 cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa gcaatgccgt
     6661 cgataacgct gataatgtcg ccaatttttt gcgagctggt agcgatctcc tgcatggtgt
     6721 gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca ttttttgcca
     6781 gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt aattgctcca
     6841 tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtgcgactt gagagatcgt
     6901 tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc ttacgcacat
     6961 cacttaccgt cccacgcaaa gcctgctgca tggtcttcag gctggcaaaa atggcggtga
     7021 tctcattgcg accatacacc gcaatcggac gtgccagatt acccgcagca atgctgtcaa
     7081 aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac cacagcgcac
     7141 tgctgatgta gatcgccgca acaataatca tgctgataaa caccagcgcc gaaatgtgat
     7201 agttacgctg gctttgcgca ctggcacttt ccagcacatg gttgatctcc agctgccagg
     7261 cctcaaagtt aacgtcaaac gcattctgtg attcctgcac cggcgcagtg aggaaatccg
     7321 aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc cagcgggcaa
     7381 aacttttctc cgtctctttt tgcaacgccc tgacgtgctc gttgcccgca gtcatcgcca
     7441 taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc gtcatcaacg
     7501 ttttaatgtc atccgccgga tagctaagcg ccgtcagagt gcccgctttg ttcagcgcgg
     7561 tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc tgattgctct
     7621 gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac atgccgttac
     7681 tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa
     7741 tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta aatactcgtc
     7801 aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt gccggtggtt
     7861 gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg gctgccagac
     7921 ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca gctgcggcgg
     7981 cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt tccgaaaccg
     8041 ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca atctcgccca
     8101 taatgtcagt cacgcgggtg acagcattga cgatattgtt cattgtttcc ccggcgcttt
     8161 cgaccagcac cgaaccggta tcaacgcgcg agacggagtc ttcaatgagg gctttgatct
     8221 cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcgcccgcc accacggcaa
     8281 aaccacggcc ctgttcaccc gctcgcgcgg cttcaacagc ggcattcagc gcgagtatat
     8341 tagtctggaa ggcaatacca tcgataacgc tgataatgtc ggcaattttc ttcgaactat
     8401 cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg tgctgggcgg
     8461 tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg ttttgcttca
     8521 ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat gcctgctgtt
     8581 ccgtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg
     8641 catctgaacc ttcacgcaca tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa
     8701 cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta ttcgccaggt
     8761 taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggtgta agcaacatac
     8821 ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg ataaccgcca
     8881 gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg cgatacagtt
     8941 tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc attccctggg
     9001 ttggctgagc gaaataagct ccagtattgc cataatcaag ataatcaatc agttcagtta
     9061 acgctgtgtg atagttttta tatttttcat caatattacg actggtagcg accatttcag
     9121 gtaacggtgc catgcttttg aattttttat aatgagtcgc tgcctgggcc aatgttttcc
     9181 tggcgctatc gagcaattca actttggcat tactttgctg attggatgaa tccatcatca
     9241 tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc catgttgacg
     9301 tcagctcgcc ctgctgttcc cgtaattgat tggaaaccac aaagctcttc tggctatggt
     9361 gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat acccccagca
     9421 ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt cctgaaaaca
     9481 agttgatctc gttatcggca aggaaggggg aaactttatt gctgatgcca cccgccgcga
     9541 aactgaaata aaaaacccga tgcgcagatc atcgggttca ttgcaattga ggaaatcggg
     9601 agaattacgc cacttctgac gccgcgctat ctaatagcgc catctcttcg ctgttcagca
     9661 gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcacccagt ccagtgagat
     9721 attctgttga aagcgtcacg gcaaattccg gtgccggacg aatttgctcc gccgtcaatg
     9781 aaagcacgtc tgagacgccg tcaaccacga tgccgaccac ccgctgtccg agattcagga
     9841 cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta attcgtaagt
     9901 ccacaatcgg cacaataaca ccgcgcagat tcgtgacgcc tttgataaac gctggcgtgt
     9961 tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcaga atatcaatac
    10021 cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc tcgctggcca
    10081 gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc aggcggcggt
    10141 gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa caatcagtgc
    10201 cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc gatagttact
    10261 ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca gcaaggcgta
    10321 gcggcgaccg ccactttgta agatcaccac aattccctgg gtagcttcgg ttttcgcgcc
    10381 cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc gcacttccag
    10441 cacccgctcg ccgccagcca gtggatgcag atcggcttca cggggttgca gtgattccat
    10501 gacagcattc agcggcagaa tgaaaacttc atccgcaacg cgtacggaca tgccgtcgag
    10561 gatggccagc gtcagcggca gtaaaatgcg gatcgtcgtg ccagtaccct gcttcgactg
    10621 gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca tgccgacgcc
    10681 gcgcccggag acgtcggtga cctgctccgc cgtggagaag ccaggggcaa atatcagcat
    10741 cgcgacttcg tcgtcgctca tgttttcgct gacggtcaga ccttgcgagg ccgcttttgc
    10801 cagaattcgc tcacggttta gccccgcacc atcgtcggtc acttcaatgc aaatgttgcc
    10861 gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg cggcgagccg
    10921 tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg tcagcgggtc
    10981 gataatgcgt tctatcaggc tcttgtcgag ttcagtggaa ctgcccacca gcgtcagttc
    11041 tacctgcttg ccgagttttc ccgcaagatc acgcaccagc cggggatagc gactaaagac
    11101 atactccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac gggcgttacg
    11161 ttgtaactgc cccatgctgg taatcaaatc accatgatta accgggtcca gttcactaga
    11221 acgctgggca agcattgact gggtgatgac cagctcgccg acgaggttaa ttaattgatc
    11281 aaccttttct accgctacac ggatgctggt ggattcactg ctgcgcgtcg ttttttcccg
    11341 ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg ggggggatat
    11401 tttgggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca caaaacagag
    11461 taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga gagaatccgc
    11521 ccctttcacc acatcagtta acgttgtcag atgccccagc tcttcttcca gcaggtcgac
    11581 ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgatcgc ggctctgctc
    11641 atcttgcggt tcacttttgg caaccacact taatcgggtc actgcagatg gcgtttcgcc
    11701 tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc tggcggcatc
    11761 cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct tcgtttccaa
    11821 aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt cgtcgagcag
    11881 gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaggttc ctgcccctcc
    11941 tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg gcgcttccgg
    12001 ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt catcaaaaaa
    12061 tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg gctgatggca
    12121 ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt actggctcat
    12181 tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc agtaccagca
    12241 ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc atcgttgccg
    12301 ccatgccgac gacacgtaac actttgccgc cattcaaccc accaaccatc agttcgcggc
    12361 gggatgcatt ggcacgatcg gcagaaagct cccagttgct gtaacctttc tcaccgctgg
    12421 cgtagggaaa atcatcggta tgacccgaga ggctaattcg gttgggaata ccgttcagta
    12481 cgggcgcgat ggcgcgcaga atgtcgcgca tatagggttc gacgtcggca ctgccagttc
    12541 taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc tggaccagat
    12601 cgattttgag atgtggacgt aacgcccgca gtttcggatc ggactcaatc aactggtcga
    12661 gatcgccacg caatttccgc aatcgacttt gctccatgcg ttttttcagc tcttcgatgt
    12721 tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccccca ccgggaattg
    12781 ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt ggagtccgga
    12841 agtactccgc aatctgaatc agctcttttg ggctggagat ggagattaac cacatcacca
    12901 gaaaaaaggc catcatcgcg gtcataaagt cggcataagc aatcttccac gatccatgtg
    12961 ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc gcttgattct
    13021 tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca catgctcttc
    13081 cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa actcaacggc
    13141 gataggcggt gcgtaaccgt tcagattaga tagcagtgtg actttgacgc actgcatcat
    13201 tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa taaatccgta
    13261 agccaataaa ataccgagga aagtccccac catcgcatgt gcgataagcg cccccagctc
    13321 ggcggcagga cgatcggctg aacctaacgc gtgaacgacc cccattacag ccgcaacaat
    13381 accaaacgcc ggaagtgagt caccgaccag cgccagactg ttcgccggga cttctgcctc
    13441 gctttcgtgc gtctcaatct cttcatccat cagagcttcg atttcgaagg tgttcatgtg
    13501 accgctgata atcaggcgca gataatcgac gataaattca agcatgacgc tatccgcaag
    13561 gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat cacgttccag
    13621 cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag ccagcagatc
    13681 catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg ccttcagcgt
    13741 gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg caccggcaat
    13801 aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca tcaaataacc
    13861 gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga catcatcctt
    13921 ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacacgcgat aggggcaaaa
    13981 aaaagcagcg gtacgtcgtt accgctgctg gaatgtttcg ccacaccgta tcagttaaac
    14041 agcctgtact ctctgttcat ccagcagttg tgggataata tcggcaggat tctgggaaag
    14101 tttacgtctt tttactgccc gggatggcgg ctgacataag ctgcaggcaa agctgccaac
    14161 aggctggtga gcgtgggtaa taaaattgcc gccgcagcag ttgcagctgg aaagttgcag
    14221 taatccactt tcaacaaacc gcaccaatgt ccaggcacgg gttaatgcca gcagcggtcc
    14281 ctcttctgct tgcgggcact gttcaaggta taaacggtag gctttgatca ccgcatcgac
    14341 gccattacac aaaccggttt tcagtaaaaa ctgccatgcg ttacagaaca tcgaagcatg
    14401 aacgttttgt tcccaggtca taaaccagtc ggttgagaat ggcagcatgc ctttcggcgg
    14461 tgggcttccg cgcagttctt tatagagttt tatcaggcgt ccgcgactta actgtgtttc
    14521 gctttccagc atctgcaaac gagcgcccag ggtgatcaat tccattgcca gctgaatatc
    14581 ccgcgcttcc tgaacaatgc ttttttcact catgatcagg cccttttctt gcgcagcgct
    14641 tcttcaggct gattaacatc attcagcaag cgtgttgaga gcatgatgcc ggtatgaatt
    14701 tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag taatcgtctg gtgactgtca
    14761 aaacggaagt gacaaaccag ttgattggtt tctgccagct taaccatttg cggaagagtc
    14821 agtgccgcta acgttgtcgc catttcttca tttatgccga gacgaaacat agcggacgct
    14881 ttgtcctgaa caatcagacg ctgtgcaagt agtaaatatg acaagttgat gtcataaatg
    14941 tgtttcagca actcggaggt atgcattatt cccacccaga ataaccaact ttatttttat
    15001 gcggtctcac cgcaccccgt gatgtcgccg ggaagccccg gtaaaaaata attagcatta
    15061 gaatagttgc gataagctgc aataggcaga gcccctttgt gggatttaat aagtccttac
    15121 aaatagaagg ggttctttac acttatctaa gatttttcct aaatcgacgc aactttactc
    15181 gtcactacac gcacatacaa cggagggggg ctgcgatttt caataatgtg tgatgcagat
    15241 cacacaaaac gctcaattac ttaacataaa tgtataagtc atacttttgt tttgggtgta
    15301 tttcaatctg ttaaaaagtt tttcgcgacg ctagcaagca aaaatgaaac aggaataata
    15361 ga
//
LOCUS       DAZGHF010000113        15305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGHF010000113 DAZGHF010000000
VERSION     DAZGHF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.114"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            55..552
                     /gene="ftnA"
                     /locus_tag="ACHK3A_003696"
     CDS             55..552
                     /gene="ftnA"
                     /locus_tag="ACHK3A_003696"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO8741730.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(590..829)
                     /locus_tag="ACHK3A_003697"
     CDS             complement(590..829)
                     /locus_tag="ACHK3A_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO8741731.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1019..2230
                     /gene="tyrP"
                     /locus_tag="ACHK3A_003698"
     CDS             1019..2230
                     /gene="tyrP"
                     /locus_tag="ACHK3A_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO8741732.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2281..2946)
                     /gene="yecA"
                     /locus_tag="ACHK3A_003699"
     CDS             complement(2281..2946)
                     /gene="yecA"
                     /locus_tag="ACHK3A_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO8741733.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIESAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3142..3228)
                     /locus_tag="ACHK3A_003700"
     tRNA            complement(3142..3228)
                     /locus_tag="ACHK3A_003700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3192..3194),aa:Leu,seq:taa)
     gene            complement(3241..3314)
                     /locus_tag="ACHK3A_003701"
     tRNA            complement(3241..3314)
                     /locus_tag="ACHK3A_003701"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3280..3282),aa:Cys,seq:gca)
     gene            complement(3369..3444)
                     /locus_tag="ACHK3A_003702"
     tRNA            complement(3369..3444)
                     /locus_tag="ACHK3A_003702"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3409..3411),aa:Gly,seq:gcc)
     gene            complement(3596..4144)
                     /gene="pgsA"
                     /locus_tag="ACHK3A_003703"
     CDS             complement(3596..4144)
                     /gene="pgsA"
                     /locus_tag="ACHK3A_003703"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO8741734.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4201..6033)
                     /gene="uvrC"
                     /locus_tag="ACHK3A_003704"
     CDS             complement(4201..6033)
                     /gene="uvrC"
                     /locus_tag="ACHK3A_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO8741735.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDESKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6030..6686)
                     /gene="uvrY"
                     /locus_tag="ACHK3A_003705"
     CDS             complement(6030..6686)
                     /gene="uvrY"
                     /locus_tag="ACHK3A_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO8741736.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6982..7158
                     /gene="yecU"
                     /locus_tag="ACHK3A_003706"
     CDS             6982..7158
                     /gene="yecU"
                     /locus_tag="ACHK3A_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO8741737.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7145..7369
                     /gene="yecF"
                     /locus_tag="ACHK3A_003707"
     CDS             7145..7369
                     /gene="yecF"
                     /locus_tag="ACHK3A_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO8741738.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7436..8158)
                     /gene="sdiA"
                     /locus_tag="ACHK3A_003708"
     CDS             complement(7436..8158)
                     /gene="sdiA"
                     /locus_tag="ACHK3A_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO8741739.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCTAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8388..9140)
                     /gene="tcyN"
                     /locus_tag="ACHK3A_003709"
     CDS             complement(8388..9140)
                     /gene="tcyN"
                     /locus_tag="ACHK3A_003709"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO8741740.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(9137..9805)
                     /gene="tcyL"
                     /locus_tag="ACHK3A_003710"
     CDS             complement(9137..9805)
                     /gene="tcyL"
                     /locus_tag="ACHK3A_003710"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO8741741.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9820..10806)
                     /gene="dcyD"
                     /locus_tag="ACHK3A_003711"
     CDS             complement(9820..10806)
                     /gene="dcyD"
                     /locus_tag="ACHK3A_003711"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO8741742.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKIGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10911..11711)
                     /gene="tcyJ"
                     /locus_tag="ACHK3A_003712"
     CDS             complement(10911..11711)
                     /gene="tcyJ"
                     /locus_tag="ACHK3A_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8741743.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(11799..12350)
                     /gene="fliZ"
                     /locus_tag="ACHK3A_003713"
     CDS             complement(11799..12350)
                     /gene="fliZ"
                     /locus_tag="ACHK3A_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO8741744.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(12396..13115)
                     /gene="fliA"
                     /locus_tag="ACHK3A_003714"
     CDS             complement(12396..13115)
                     /gene="fliA"
                     /locus_tag="ACHK3A_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO8741745.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(13434..15173)
                     /locus_tag="ACHK3A_003715"
     CDS             complement(13434..15173)
                     /locus_tag="ACHK3A_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO8741746.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAATKSDLAAAQLLAPGTADANGTVTYTVSAGLKT
                     STAADVIASLANNAKVNATIANGFGSPTATDYTYNSATGDFTYSATIAAGTNSGDSNS
                     AQLQSFLTPKAGDTANLNVKIGSTSIDVVLASDGKITAKDGSELFIDVDGNLTQNNAG
                     TVKAATLDALTKNWHTTGTPGAVSTVITTEDETTFTLDGGTDATTSGAITVANARMSA
                     ESLQSATKSTGFTVDVGATGTSAGDIKVDSKGIVQQYTGTVFEDAYTKADGSLTTDNT
                     TNLFLQKDGTVTNGSGKAVYVSADGNFTTDAETKAATTADPLKALDEAISSIDKFRSS
                     LGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKA
                     NQVPQQVLSLLQG"
BASE COUNT         3737 a         3940 c         3613 g         4015 t
ORIGIN      
        1 taacaaatta tagtgacgcc acagcaacaa atataacctt tgtggagcac tatcatgctg
       61 aaaccagaaa tgattgaaaa acttaatgag cagatgaacc tggaactgta ctcttcactg
      121 ctttatcagc aaatgagcgc ctggtgcagc tatcatacct tcgaaggtgc tgccgcgttc
      181 ctgcgccgtc acgcccagga agagatgacg catatgcagc gtctgtttga ttacctgact
      241 gataccggca acttaccgcg tattaatacc gttgaatctc cgtttgctga atattcctca
      301 cttgatgaat tattccagga aacctataaa cacgaacaat taatcacgca gaaaattaac
      361 gaactggctc atgctgcaat gaccaatcag gactacccaa catttaattt cctgcaatgg
      421 tatgtttctg agcagcatga agaagagaaa ctgttcaaat cgattattga taaattaagc
      481 ctggcaggca aaagtggcga aggtctgtat tttatcgaca aagaactctc taccctcgac
      541 acacaaaact aatgctctcc ggcggcaggt tttctgccgc cgtttcaaat cagtgacggc
      601 aaatcttact ctgatcggtt gaaaaacctt cttctgaagg tataaatttg ccttttgctg
      661 ccagaaacgc caccaactct cccgcagtca tcccttctgc cgagcaggtg tgaaaacgtg
      721 cctgttcacc aaaacgcgct ttaattgctg cttccagact ggcatgcgta tattgctcgc
      781 ctgattcaat catcatattt aacacttcgt gaccgtgaat agagtccatc gttcctcctc
      841 aaaaaaagcc tagcgtagcg attgccgctt atgaagactt tgcgccagcg cagaactgaa
      901 cgcttttatt gtacatttat atttacatca tatgtaacgc cggtttgacg aatcagccgt
      961 tatgccttaa cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt
     1021 gaaaaacaga actctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat
     1081 gctggcaatg ccgctggctg cggcaggtgt tggttttagc gtcacgttaa tcttgttaat
     1141 tgggctttgg gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt
     1201 cccggcagat accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcagtg
     1261 gctgacgggc ttcagtatga tgttcttaat gtatgctctg actgcggcat acatcagcgg
     1321 tgccggtgaa ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac
     1381 cgctggcgtg ctgttgttca cttttgttgc cggtggcgtg gtttgtgtcg gaacttcgct
     1441 ggtcgattta tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct
     1501 ggtattgctg ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg
     1561 actggcactg tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc
     1621 gagtattgtc agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg
     1681 tagtgcgatc cccctggtgg catatatttt ctggcagctg gcgacccttg gcagcattga
     1741 ttcaacaacc tttatgggat tgctggctaa tcatgccgga ttaaacgggc tgttacaggc
     1801 gttacgcgaa atggtggcct ctccgcatgt tgagctggca gtgcatttat ttgctgattt
     1861 agccctcgcc acgtccttcc tcggcgtagc gttaggctta tttgattatc tggcagattt
     1921 gtttcagcgt tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc
     1981 gccactggcg tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg
     2041 tgtggcgctg gcggtactgg cattgattat cccttcgctg ttgacctggc aaagcaggaa
     2101 gcacaatcct caggcgggtt accgggtgaa aggtggtcgt ccggcgctgg tggtggtgtt
     2161 tctctgtggt attgctgtga ttggcgtgca atttttgatt gcggcaggct tgttaccaga
     2221 agtggggtga ttagattctt attgggtgcc agaattaacg ctgacaccca atttgacctc
     2281 ttaatgcagg cagcactgct taaatttctt accactaccg cacggacaag gatcgttacg
     2341 ccccggtttc tcttctgctt tgataggttg ctgaacagct ttttcctgcg gatgcgccat
     2401 ccagtatgca tgtaaatcaa gcgccgccag tcgaatagca tctacgctct cttcaaacgc
     2461 ttctggcgac atcttctcta cccgctcgaa gttttcctca gtaccgtgca gcgcaatcgc
     2521 atccagcgct ggttttaacg aatcgggcaa cgttgaccag tcagaaagtg ccacgccccg
     2581 catatagcca aagcaccact cctcaacaat cgtcagctcg ctgccatcga cttctcgcaa
     2641 gccgaataac ggctcaaact gctccgggaa ttcgttcaga cgctctgcgg tatcagccat
     2701 atgttgaaaa gccaggttca taaagcgcgt catctctttc tctgacgccc agcgcggcac
     2761 atagtcagcc ccaccccaca cggcaaccag ccactgtgcc gattcaatct cttgcggaga
     2821 actcaacacc gccgtcaata aaccgtccag ctccgccaca tcaagaatgg cgtggtcagt
     2881 gttgtatttg gtcagaatat cgtccagcca ttccaactca ctttcgttta acggtcccgt
     2941 tttcatacgc ttttccttgt ggatctcaac tcgccagcac ctatcttaca tgccggtccg
     3001 tatcagagat actttttgag tggctttgcc ggtgattaaa aattaaggag ggtgtaacga
     3061 caagttgcag gcacaaaaaa accacccgaa ggtggtttca cgacactgct tattgctttg
     3121 attttattct tatctttccc atggtacccg gagcgggact tgaacccgca cagcgcgaac
     3181 gccgagggat tttaaatccc ttgtgtctac cgattccacc atccgggctc gggaagaaag
     3241 tggaggcgcg ttccggagtc gaaccggact agacggattt gcaatccgct acataaccgc
     3301 tttgttaacg cgccaaattc ttcaggcctt tcagccagac atccgcttga cgccgatgtc
     3361 ttttaaactg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
     3421 ctctaccaac tgagctattc ccgcattcat caagcaatca gttaatcact tgattttatt
     3481 atcgtctggc aatcagtgcc gccgttcgat gcgttgcatt ctacttacct ggcgcgatga
     3541 gtcaacgata tttttcacca cttttgatcg tttgctgaaa attgcgccga aacgatcact
     3601 gatcaagcaa atctgcacgc gcagcgctca aatattgcaa cattgaccac agagtcagta
     3661 ccgcagccac aaagaaaagt gcaataccgg cgtactcaac ccaaatgttc ggacgccaca
     3721 gcagccaggc cagcgccacc atctgggcag tggttttcac tttcccaatc caggagacag
     3781 ccacgctact gcgtttaccc aactccgcca tccattcgcg taacgcagaa ataataattt
     3841 cacgggcgat catcgttgct gccggtaagg tcacccacca gctgtgataa tgctcggtta
     3901 ccagcaccat ggcgatagcc acgagaactt tatctgctac agggtcaagg aaagcaccaa
     3961 accgggtact ctggttccag cggcgtgcca gaaaaccatc gaaccagtca gtcaccgccg
     4021 cgacgcagaa aatgagcgcg gcggcaaacg gcgaccaggt gacaggcaga taaaagacca
     4081 atacaaagaa tgggataagg atgacacgga acagtgtaag caacgtaggg atattaaatt
     4141 gcataatgac gggtaactat ctgttgtcag taagattacc cctatgttgc tacagagaca
     4201 tcaatgtttc aacgaccaga agatcttttc tgccagacct tgcgaaatac ccggcacttt
     4261 tgcaatttcc tcgacgctgg cgttacgtaa accttgcaaa ccgcccatat atttcaacaa
     4321 catttgccga cgttttggcc cgacgccttc aatggtttcc agggaactgg tatttttgac
     4381 cttcgcccgt tttttacggt gcccgccaat cgcgtgatcg tgtgattcat cgcgaatatg
     4441 ctggataaca tgcagtgcgg gagaatctgg cggcaaactg aatccctcac cttccggctc
     4501 aaagaacagc gtttccagcc cagccttacg atctgctcct ttggcaacgc ctagtagcag
     4561 cggatgattt ttatcccatg agacatccag ttcggcgaaa acatttttcg cctgcgcaag
     4621 ttggcctttg ccgccgtcga taaggatcac atccgggatc ttactctcgt cgatggcttt
     4681 accataacgc cgacgcagca cctgattcat cgccgcataa tcatcgcccg gcgtgatgcc
     4741 agtaatgtta tagcgtcgat actccgcacg cagcgggccg ttagcatcaa acaccacaca
     4801 ggaagcgacg gtttgttccc ccatggtatg gctgatgtca aagcattcca tccgcttcac
     4861 ttccggcaat ttcaacacgc tggcaagcgc ggtcagtcgc tggtgaacgg tagattgctg
     4921 cgaaagtttg ctggttaagg ccgtcgccgc attggtgcgc gcgagtttca gataacgcgc
     4981 ccgatcgccg cgaggtttgg tttgaacatt aatcttgcgt cccgccagtt ctgaaaggga
     5041 atcggcgagc agcgttttat cgctaagatt aaaatcgagc aggatctcac ccggtaaggt
     5101 gcgcatctgg ctgccttgta aatagaactg acctacgaag gtttccacca cctcgctcag
     5161 ttccgtaccg ccaggcactt tcgggaaata gctgcggctg ccgagcactt taccctgacg
     5221 aatgaacaat acgtggacac aagccatgcc cgcatcgaac gccacaccaa taacgtcgag
     5281 gtcgtcgccg gtattggaca caaactgttt ttcggtgacg cgtcgcaccg cctgaatttg
     5341 gtcgcgaata cgtgccgctt cttcaaactc cagattctgg ctggcagttt ccatacgact
     5401 aatgagttgc gtaagcacct gatcatcttt gccagacaaa aacaggcgca catactcgac
     5461 ctgctgagcg tattcttctt cactcaccag tccttcaacg cacggtccca gacagcgccc
     5521 tatctgatat tgcagacacg gacgcgagcg attgcgataa acactatttt cgcactggcg
     5581 aatggggaat atcttttgca gtagcgccag tgtttcacgt acggcatagc cattcgggaa
     5641 cgggccgaaa tattcacctt tagcatgctt cgcaccacga tgcatcgcca gacgcggatg
     5701 ggtatcgcca ctcaggaaga taaagggata agatttatca tcgcgtagca aaacgttgta
     5761 acgcggctga tagagtttga tgtagttgtg ttccagcaac agcgcttcgg tttctgtatg
     5821 agtaaccgtt acatcaattt gctggatctg ggcgaccagc gcttcggttt tgcgcgaagc
     5881 gaggttgcta cggaaatagc tggaaagccg ttttttcagg tctttcgctt tgccgacata
     5941 gataaccgta ccaccagcat cgtacatgcg ataaacgcct ggctggctgg ttacggtttt
     6001 taaaaacgct tttgcgtcaa actgatcact cactgacttg ataatgtctc cgcattacac
     6061 agaccatggc gaattgccag gtgagtcagc tcaacatcgc catgaatgtt tagtttactg
     6121 aacatacgat agcggtagct gttcaccgtt ttcggactga gattgagctg ttctgagatc
     6181 tcattgacct tctggccctt ggtgatcatc agcataatct gcaattcacg ttcagacaaa
     6241 ctggcaaatg ggctttctgt tttttctggt tcgatctggc ttaacgccat ttgttgagcg
     6301 atgtcagaag caatgtaacg ctgccctgaa tagacagaac ggatcgcact cacaacttcc
     6361 tgcggagccg cgcctttgct gaggtagccc gcagcaccgg cctgcatgac tttcgctggt
     6421 aaagggtttt ctgtatggac ggtaagcatg atgattttaa catcagctgt ggaacgcgcg
     6481 attttacgcg tcgcctcaag gccgccaatg cccggcatac tcatgtccat tagcaccacg
     6541 tcaacggcat ttgcccggca ccatttaacg gcgtcttcac cgcacgatgc ctctccgacg
     6601 acttttatac cctttatatc ttccagaatg cgtcgtatcc ctgcgcgcac cagttcgtgg
     6661 tcatcaacaa gtagaacgtt gatcaaagga atatctccag aaatagggat aacgctactg
     6721 atagttagtc attcgtatat tagcggtttt tattgcaact ttgaaacgtt aaaaatggtg
     6781 ggtattcgat ttttctctcg tttttgtaaa ttcgattgtc cacagttatg gtcacgcccg
     6841 tcaccaacgg cttccgggat cgtttcactg taagccctgc gatgaaatgt gacaaaaatt
     6901 gactttattc agcaaaaatg aaaatcagcc cgctgaatat gtaacattaa ttaacccgaa
     6961 caatacgggc aaaaaacatt catgataaaa atatttattg gtcattatat taacgtattt
     7021 tatagcactg ccgatatcac gctcaaaaaa caaccactgc tatttttagc aaagcttatg
     7081 gtatactccg ccgccttaac atttttcacc gcaaattttc attgcaacat gacgaggaaa
     7141 ataaatgagt acgcctgatt tttctactgc cgagaataat caagaactgg caaatgaagt
     7201 ctcctgcctg aaagcgatgc tgacgctgat gctgcaggcg atgggacaag ctgacgcggg
     7261 ccgcgtgatg ttaaagatgg aaaaacagct tgcgctgatc gaagacgaaa cccaggctgc
     7321 agtattttcc aaaacggtta agcaaattaa acaagcctac cgtcagtaat ataaaaccgg
     7381 ctgagagcgt gcctttcagc cggtttttgc atctggcacg cagaaagaaa agcgatcaaa
     7441 ttaagccagt agcggccgcg taacaggcca cctgggtctt atttggtgca ttgatttttt
     7501 tctgcatgtt tttctgatgg aaattgaccg tattctcaga gattgacaaa atcatcgcta
     7561 tctctgctga tgtcttccct tccgccgtcc atttcagaat ttctttttcg cgcttgctga
     7621 aattcatctc tggcgtcatc actatttcat catttaaacg catcagagcc atcagacttt
     7681 cgcgcaccag taactgcatt tttaattgca gttcatcact aagaatgggt atttcgcgcg
     7741 cagtgcaacg ggaaaaggac aaaaagccca gcgcccggtt tggcagcatt aaatactgag
     7801 tgacaccgcg gcgtaaacca tgcgcgcgcg cagcttccca taacggctgt gcttcgctga
     7861 ataagtcatc attccacatt aaatggccct gactaaagtt ttcagggttc agtacaggat
     7921 caattgcgag aaagtttttt gcctgataat aactaaccca cgcctcaggg taattggtgt
     7981 agaaagccac tttaggtcgc gtgaatggta ccgggtggcg aacacataac gaatagtaat
     8041 cgtactccag ctgctgagcc tgaagctcta tttcatggta gacctcttct gcggcctcca
     8101 tcctctgaaa acgcaacagc atcgtgcgac gccagctgaa aaaatccgta tcctgcatag
     8161 taaaccgcaa cgcccctgag agtgagtatc atttataatg ataattcaaa ctatcacata
     8221 aataagattt atatataatt tatattattc aggcaatgaa ttacttttgc aagccatcgc
     8281 attctcttat gttattaatg agttatgctg atttgttaag cagttttatc acgtttgaag
     8341 tggcgcaccg ccccgacagg tgaatcgtcg gggctgattt tttcttatta ttgcagcaga
     8401 aacttctcga ggaactggcg ggtgcgaggc tgctcgggat cggcaaataa cgcttttgcg
     8461 gccccctgct cgactatccg cccctggtcc ataaagatcg cccggtccgc aacatcccgg
     8521 gcaaagctca tttcgtgcgt cacaatcacc atcgtgcgct tttcctgcgc cagctggcgg
     8581 atggtgttca ggacttcgcc caccagctct ggatccagcg ccgacgttgg ctcgtcaaac
     8641 aaaatcactt caggacgcat agccagcgct cgcgcaatcg cgacacgctg ctgttgaccg
     8701 ccagacaaac gacgtggata gctggtttct ttacctgcca gcccaacttt cgccagcagc
     8761 tcgcgagcgc gcgccgtggc ctcttctttc ggttcgcctt tgacgatcac cggcccttca
     8821 ataatgtttt ccagcaccgt acgatgcgga aacaaattaa agttctggaa gacaaacccg
     8881 acgtgctgac gcaactggcg aatcagcgat ttttgctgac ttaatgaacg tgcagtatca
     8941 atggtgatat cgccaacggt gatggttccc gcttccggtt gttccagcag atttatgctg
     9001 cgtagcaacg tggttttgcc ggaaccactt ggaccgataa ttgccaccac ttcgccaggc
     9061 ttcacctcaa ggtcgatacc gtgcagcacc gtctgaccgt ggaatttttt caccaggttc
     9121 ttaacttcaa tggcactcat tttggttctc tctcctggcg attaagttga ttctcaaaat
     9181 ggttctgcaa cgtcgataat actgtcgcca tgatccagta gatgagcgaa gccgccaggt
     9241 acatggtgaa cacctccagc gtgcgcgagg taatcaactg cgcctgacgg aacagctccg
     9301 gcacctggat tgtcgcggcc agcgacgtat cttttaccag gctgataaag ctgttcgaca
     9361 gcggcggcaa cgccacacgc gccgcctgtg gcaaaatcgc gcgacgcatg gtctgccacg
     9421 gcgtcatacc aatactggca gcagcttccc actgaccttt atcaatagaa gaaatggcgg
     9481 cgcgcagtgt ttcggcggca taggcggcag tgtttagcga cagaccaatc attgctgacg
     9541 gaatgggatc taattcgatg ccaaactgcg gcaggccgta atagatcata aacagttgtg
     9601 cgatgagtgg cgtaccacga aaaatagaga tataaaaacg cgccagccag cgcacgggcc
     9661 agattggcga aaggcgcatc agcgcgagaa taaacccgag cagtaagcca aaaaacatgc
     9721 cgccgatact gagttgcagc gtatacccag cccctttcag caggaacggc aaggaatcaa
     9781 taaccagttg tatactttct tgcatgagcg tttccggagc taaacgtggg gatgataggc
     9841 gaacagcgca ggcgcgccgc cggtatgaat aaacagaatc ggcccttcat ctttgaagcg
     9901 tttctgactg ataccgtcaa tcagccccgc cattgctttt ccggtataca caggatcaag
     9961 cagaatgcct tccagccgcg ccagcaattt cactgcttcc atgccttcgt cgttcggcac
    10021 gccgtagcca ggtgcaaaat agtcatccca gagtaaaatt tccgctgatg cggtcagctc
    10081 cagttctttc gcaatcgcct gttggaggtt aaccactttc ggcaattgat cggcaacgga
    10141 acgcgacacg gtcacgccaa tcagttcgct ttcaggcatc aggtgttcca gcccaacagc
    10201 cagtccggcg tgagttccgg cactgcccga cgcgaccacc accgacgaaa tattaactgc
    10261 cccttcacac tgttgcgcaa tttccagcgc actctccaca taacccagcg cgcccaaagc
    10321 attagaaccg ccaaccggaa tgacatacgg gcgaaagcct tgtgcttcga ctcgcgtcgc
    10381 cagctcttcc agttgggcat tgggatcggt cagtgcgtcg cacatttcaa tctgggtatt
    10441 gaacagatcc agcaacaaac gattgccgtt ggttaaatag ttttctgcgg ttgtgccaat
    10501 aggattttcc agcagcgcca cgcagtgcag accgattttc gccgcaactg cggcagtctg
    10561 gcgcacatgg ttagactgga tcgccccggc agtaatcagc gtatcggcac cttcacgcag
    10621 agcatctgcc gcgagaaatt ccagcttacg taatttattg ccgcccattg ccatgggagt
    10681 gacgtcatcc cgtttgatga aaatttcccg tccaagataa tcagaaaagc gcggcagata
    10741 ttcgagcggc gttggcgcgc cgataaactc cagccgtgga aaacgggtta aattatgcag
    10801 tggcataaca gcctccgatg tgtgttgttg tgattttctt attatgcacg ctgaaaacgc
    10861 gtaaataaaa aaggcgctag tgaaagcgcc cttttttgtc attatgctga ttatttggtc
    10921 acatcagcac caaaccattt ttcggaaagg gcttgcagag tgccatcttt ttgcatttcc
    10981 gcaattgcat cattcaccgc tttcagcagg tcttcatttc ctttacgcag cgccacacca
    11041 gactcctgac gggagaatgc ttcaccggtt actgccagcg tatcgttggt tttcttcacc
    11101 agatccagcg ccgccagacg gtcaacgagg atcgcatcga tacggcctac gcgcagatcc
    11161 tgatatttgg tcgggtcatc atcataggta cgcacatcga cgccctgaac attctgacgc
    11221 agccactctt catagttggt gcccagaccg acacccactt ttttgccttt cagatcatca
    11281 gccgttttaa tggtgccttc gttacctttt ttcaccagcg cctgaatacc ggaaatggtg
    11341 tacggggtag agaaatcgta ttttttcttg cgctcgtcag aaatggtgac ctggttaatc
    11401 accacatcaa tgcgtttaga gtccagcgac gccagcatac cgtcccattt agtcggtttt
    11461 agtgacgcct caacgccaag atgttttgcc agctgttggg caaattccac ttcaaaaccg
    11521 gttaatttgc cgtcatcccc ctgaaaactg aacggcggat aagttccttc cagccctacc
    11581 agcagcgtgc cgcgctcttt gactttatta agcagacctt catctgcaaa acttttaacg
    11641 ctcatacccg caaccagcgc cacggccatc acacccatca atgcctgacg tcccagatgt
    11701 gctaatttca tatttacccc gaatgttgtt atgtctgttt gcagtgtaga gccattgttt
    11761 gtaaacacaa aaacaactcc gctacatctt attcttattt aatatatatc agaagcaggc
    11821 agactggagg atttctgcac aagtccggta cacgtttgac gctgatagtg ttgatacttc
    11881 cgtaacgcaa tgcggtaatt gttggtgctg gtggtgggca gccacggggc gaggatttca
    11941 tcaaggaaac cgtcctgcag ctgatcgagg gaaatatttt gctcgtgtag atgattaccc
    12001 aaacggcgca gacgaacgac atattcacgc accgtacctg ggctcatttc agtttgctca
    12061 aaaagaaatt gcttaaagcc gataatatcg aaaaaatcac tctgcgtttt gcaatgtaaa
    12121 tcaccgcaaa atcggcacaa tgccgcccat gatttttgtt ccacttgcca gccattttca
    12181 tcaagcagca tgtccaggcg ggaaatctcg attttattca ctattttgcc gtcgcgaacg
    12241 agggtaatgc gatcgagtaa tttacggcaa tgcgcgcaat gggtctggct gtgtttaaag
    12301 tctttaagat agcggcttaa tggccgtctt ttcaggtgct gcaccatcat taagaactcc
    12361 tggtagtcaa aattaaagtg cggcatttac tgacgttata acttacccag tttagtgcgt
    12421 aaccgtttaa tggcctggct gtgtaactga ctgacccgcg attccccgac ctccagcacc
    12481 gcgccaatct ctttgagatt cagctcttcc tggtaataga gggttaatac cagtttttcg
    12541 cgctccggca acgtttcgat agcttccatc acccgctggc gcagattact gtccagcagt
    12601 tgttgtagcg ggttttctcg ctgatgatca tcagtaacca gttcgatgct atcgccgtgc
    12661 tcttcgcgcc actcatcgta ggagaagagc tggctgttat tggtgtcgag caacatttgg
    12721 cgatagtcgg caatatcgat ccctaaacgt tccgctacct cagtttccgt ggcgttgcgg
    12781 ccaagttcct gctccagttg ccctattgcc tgtgccactt cacgcgcgtt gcgtcgcacg
    12841 ctgcgcggca cccagtcacg gctgcgaagt tcatccagca tagcgccacg gatacgctgc
    12901 actgcgtaag ttgtaaatgc cgttccttgt agggcgtcat agcgttcgac ggcattgagt
    12961 aacccaatgc cgcccgcctg tagcagatcg tcaagttcca cgctcgcggg cagtcgaacc
    13021 tgcaggcgca atgcttcgtg acgcaccagc gggacataac gctgccacag cgagtgttta
    13081 tccattacac cttcagcggt atagagtgaa ttcacgataa acagccctgc gttatatgag
    13141 ttatcggcat gattatccgt ttctgcaggg tttttaatcg gacgattagt gggtgaaatg
    13201 aggggttatt tgggggttac cggtaaattg cgggcagaaa aaaccccgcc gttggcgggg
    13261 aagcacgttg ctgacaaatt accattcatg ttgccggatg cggcgtaaac gccttatccg
    13321 gcctacaaaa tccgcaaatt caataaattg caattcacct tgtaggcctg ataagcgcag
    13381 cgcatcaggc aatttggcgt tgccgtcagt ctcagttaat caggttacga cgattaaccc
    13441 tgcagcagag acagaacctg ctgcggtacc tggttagctt ttgccagcac ggagttaccg
    13501 gcctgctgga tgatctgcgc tttcgacatg ttggacactt cggtcgcata gtcggcgtcc
    13561 tgaatacggg actgcgcttc ggacaggttg gtagtggtgt tgttcaggtt ggtgaccgcg
    13621 gaatccagac ggttttgcac cgcaccgagg gaggagcgga atttgtcgat ggagctgatg
    13681 gcttcgtcca gagctttcag tggatcggcg gtggttgcag ctttagtttc agcgtcagta
    13741 gtaaaattac catccgctga aacatagact gctttacctg aaccattggt cacagttccg
    13801 tctttttgca aaaacagatt ggttgtatta tcggtagtca gtgaaccatc agctttggtg
    13861 taagcgtctt caaaaactgt acctgtgtat tgttgtacta tacctttact atcaacttta
    13921 atatcgcctg cgctggtacc agtagctcca acatcaactg tgaatcctgt ggacttagtt
    13981 gccgattgaa gagactcagc actcattctt gcatttgcta cagtgattgc accagaagta
    14041 gtagcatcag taccgccatc cagagtgaag gttgtttcat cttcagttgt aattaccgta
    14101 gatacggcac ccggtgtgcc tgttgtatgc cagtttttag tcagtgcatc aagagtggct
    14161 gctttgacag tcccagcatt gttttgagtg aggttaccat ctacgtcaat aaatagttct
    14221 gaaccatctt tcgcggtaat tttaccgtcg ctagccaata caacgtcaat tgacgtagaa
    14281 ccaattttaa cgtttaagtt agcagtatcg cccgcttttg gtgtcaggaa ggattgtaac
    14341 tgagcactgt tactatcacc agaatttgta ccagctgcaa tagttgcact atatgtaaaa
    14401 tcacctgtag cgctgttgta tgtataatct gtagctgttg gcgatccaaa accatttgca
    14461 attgtggcat taacttttgc gttattagcc aaactcgcaa ttacatctgc agctgtagat
    14521 gttttcaggc ctgcgctaac agtataggta actgtaccat tagcatcagc agtacctgga
    14581 gccaagagtt gagctgctgc caaatcagat ttagtcgctg cagtattagc cacagccccg
    14641 ccaccattca cattaaaccc actcagcccc agcgtatcag agtcaatttt cttcagatca
    14701 atagtgatag tctggccgtc attcgcacca acctgaattt tcattgaacc gtctttcgcc
    14761 agtacgttca cgccgttgaa ctgggtctgg ccagatacgc ggtcaatttc gtccagacgg
    14821 gatttgattt cgtcctgaat ggagtccaga tccgaatcgg agttagtccc ggtagaagcc
    14881 tgaaccgtca gttcacggat acgctgtaag ttgttgttga tttcggacag cgcgccttcg
    14941 gtggtctgtg caacggagat accgtcgttg gcgttacgtg cagcttgagt cagaccttta
    15001 atgttagaag taaaacggtt agcaatcgcc tgacctgcgg cgtcatcctt cgcgctgtta
    15061 atacgcaagc cagaagacag acgctcgata gaactcgaca gcgcagactg gttcttgttg
    15121 atattatttt gagtgatcag cgagaggctg ttggtattaa tgacttgtgc catgattcgt
    15181 tatcctatat tgcaagtcgt tgattacgta ttgggtttcc acccgtcggc tcaatcgccg
    15241 tcaaccctgt tatcgtctgt cgtaaaacaa cctttagaat ttttttcaca aacagccatt
    15301 ttttg
//
LOCUS       DAZGHF010000114        15228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGHF010000114 DAZGHF010000000
VERSION     DAZGHF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.115"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            2..1360
                     /gene="chbC"
                     /locus_tag="ACHK3A_003716"
     CDS             2..1360
                     /gene="chbC"
                     /locus_tag="ACHK3A_003716"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO8741747.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWTLNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            1411..1761
                     /gene="chbA"
                     /locus_tag="ACHK3A_003717"
     CDS             1411..1761
                     /gene="chbA"
                     /locus_tag="ACHK3A_003717"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO8741748.1"
                     /translation="MMDLDNIPDTQTEVEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKTMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1769..2611
                     /gene="chbR"
                     /locus_tag="ACHK3A_003718"
     CDS             1769..2611
                     /gene="chbR"
                     /locus_tag="ACHK3A_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO8741749.1"
                     /translation="MMQPVINALEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2715..4067
                     /gene="celF"
                     /locus_tag="ACHK3A_003719"
                     /gene_synonym="chbF"
     CDS             2715..4067
                     /gene="celF"
                     /locus_tag="ACHK3A_003719"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO8741750.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            4080..4838
                     /gene="chbG"
                     /locus_tag="ACHK3A_003720"
     CDS             4080..4838
                     /gene="chbG"
                     /locus_tag="ACHK3A_003720"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO8741751.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSLDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGCYRDV"
     gene            complement(5096..7357)
                     /gene="katE"
                     /locus_tag="ACHK3A_003721"
     CDS             complement(5096..7357)
                     /gene="katE"
                     /locus_tag="ACHK3A_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO8741752.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            7540..7803
                     /gene="cedA"
                     /locus_tag="ACHK3A_003722"
     CDS             7540..7803
                     /gene="cedA"
                     /locus_tag="ACHK3A_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO8741753.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(7850..9241)
                     /gene="tcyP"
                     /locus_tag="ACHK3A_003723"
     CDS             complement(7850..9241)
                     /gene="tcyP"
                     /locus_tag="ACHK3A_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO8741754.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(9374..9964)
                     /gene="ydjM"
                     /locus_tag="ACHK3A_003724"
     CDS             complement(9374..9964)
                     /gene="ydjM"
                     /locus_tag="ACHK3A_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8741755.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(10128..10796)
                     /gene="hxpB"
                     /locus_tag="ACHK3A_003725"
     CDS             complement(10128..10796)
                     /gene="hxpB"
                     /locus_tag="ACHK3A_003725"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO8741756.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYIDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            10943..11479
                     /gene="yniB"
                     /locus_tag="ACHK3A_003726"
     CDS             10943..11479
                     /gene="yniB"
                     /locus_tag="ACHK3A_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO8741757.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFFLIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(11520..12380)
                     /gene="yniA"
                     /locus_tag="ACHK3A_003727"
     CDS             complement(11520..12380)
                     /gene="yniA"
                     /locus_tag="ACHK3A_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO8741758.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(12486..12776)
                     /gene="ghoS"
                     /locus_tag="ACHK3A_003728"
     CDS             complement(12486..12776)
                     /gene="ghoS"
                     /locus_tag="ACHK3A_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO8741759.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            complement(12877..13806)
                     /gene="pfkB"
                     /locus_tag="ACHK3A_003729"
     CDS             complement(12877..13806)
                     /gene="pfkB"
                     /locus_tag="ACHK3A_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO8741760.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPVGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            14093..14851
                     /gene="ydiY"
                     /locus_tag="ACHK3A_003730"
     CDS             14093..14851
                     /gene="ydiY"
                     /locus_tag="ACHK3A_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO8741761.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(14904..15011)
                     /gene="yniD"
                     /locus_tag="ACHK3A_003731"
     CDS             complement(14904..15011)
                     /gene="yniD"
                     /locus_tag="ACHK3A_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO8741762.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         3841 a         3575 c         3856 g         3956 t
ORIGIN      
        1 tatgagtaat gttattgcat cgcttgaaaa ggtactcctc ccttttgcag ttaaaatagg
       61 aaagcagcca cacgttaatg caatcaaaaa tggctttatt cgcttaatgc cgttaaccct
      121 tgcgggggcc atgtttgtat taattaacaa cgtttttcta agctttgggg aggggtcgtt
      181 tttttattcc ttaggtattc gcctggatgc ctcaaccatt gaaacactta atggtctgaa
      241 aggtattggc ggcaacgtat ataacggaac attagggata atgtctttaa tggcaccgtt
      301 ctttattggc atggcgctgg cagaagagcg taaagtcgat gcgctggcgg ccgggttgtt
      361 atccgttgca gcatttatga ccgtcacccc atatagtgtc ggtgaggcct atgcggttgg
      421 cgcaaactgg ttaggtgggg cgaatatcat ctccgggatt attattggcc tggtggtggc
      481 agaaatgttt acctttattg tccgccgcaa ttgggtcatt aaattgcccg atagcgtacc
      541 tgcttcagta tcgcgttcct tctcggcttt aattcccggc tttattattc tttccgtgat
      601 ggggattatt gcctggacgt tgaatacctg gggcaccaac ttccatcaga ttattatgga
      661 taccatctca accccactgg catcgttggg tagcgtggtg ggctgggcct atgtgatctt
      721 tgttccactg ctctggttct tcggtattca tggtgcgctg gcgctgaccg cactggacaa
      781 cggcattatg acgccttggg cgctggaaaa tatcgcgacc tatcagcaat atggttccgt
      841 cgaagcggcg ctggcagccg gtaagacctt ccatatctgg gccaagccga tgctggactc
      901 ctttattttc cttgggggca gtggtgcgac tttaggtctg atcctggcta tctttatcgc
      961 ctctcgccgt gctgattatc gtcaggtggc aaaactggcg ctgccgtccg gcatcttcca
     1021 gattaacgaa ccgattctgt ttggtctgcc aattatcatg aacccggtga tgtttatccc
     1081 gtttgtactg gtacaaccga ttctggcggc aatcaccctc gcagcgtact acatgggcat
     1141 tattcctccg gtgaccaata ttgcaccgtg gaccatgcca accggtctgg gagccttctt
     1201 taacaccaac ggtagcgtcg ccgcattgct ggtcgcactc ttcaaccttg gtatcgcaac
     1261 gttaatttat ctgccctttg ttgtggtggc taacaaagca caaaacgcga ttgataaaga
     1321 agagagcgaa gaagatatcg ctaacgccct gaaattttaa tcgatgccgg tacgggtaat
     1381 cgtgccggta agaatagaga ggaacgatgt atgatggatc tcgataatat tcccgatacg
     1441 caaacggaag ttgaagagct ggaagaagtg gtgatggggc ttatcatcaa ctccggacaa
     1501 gcgcgcagcc tggcgtatgc ggcactgaaa caggcgaagc agggcgattt tgccgcagca
     1561 aaaaccatga tggatcagtc acggatggca ttgaatgaag cgcatctggt acagacgaaa
     1621 ctgattgaag gcgatgcggg cgaaggtaag atgaaagtga gtctggtgct ggtccacgct
     1681 caggatcatt taatgacgtc catgcttgcg cgtgaactga ttactgaatt aattgagctt
     1741 catgaaaaac tgaaggcata aggagtcgat gatgcagcca gtgattaacg cgctggaaat
     1801 tgccactgcc cgagaacagc agttgtttaa tggcaaaaat ttccatgtgt ttatctacaa
     1861 taaaactgag agtatcagcg gactgcatca gcacgactat tatgaattta ctctggtatt
     1921 aaccgggcgt tatttccagg agattaacgg taagcgcgtg ttactggaac ggggcgattt
     1981 tgtttttatt ccgttaggtt cgcaccatca aagtttttat gaatttggtg ccacgcgtat
     2041 attgaacgtt gggatcagta aacgcttttt tgagcagcat tacctgccgt tgttgcctta
     2101 ttgctttgtc gcttcgcagg tataccggac caataacgct tttctcacct atgtggaaac
     2161 agtgatttct tcattgaatt tccgcgaaac agggctggaa gagtttgttg agatggttac
     2221 tttttatgtc attaaccgtt tacgtcatta ccgcgaagaa caggtgattg atgatatacc
     2281 gcagtggctg aaaagtacgg tagaaaagat gcatgataaa gagcagttta gtgaatcggc
     2341 gctggagaat atggtgacgt tgtcagccaa atcacaggaa tatttgacgc gagcgactca
     2401 acgatactat ggtaaaacgc caatgcagat tattaatgaa atccgtatta attttgccaa
     2461 aaaacaactg gaaatgacca actattcagt gacggatatt gcgtttgagg ccggttatag
     2521 tagcccgagc ttgtttatta aaacgtttaa gaaattaaca tcctttacgc ctaagagcta
     2581 tcgtaagaaa ttgactgaat ttaatcagta agtcgttata cctgacaatt cacatatctg
     2641 tcctgttgct ggattattta tgtccggggg gcagatatgc cagatatcag tattctgtac
     2701 tgaagggaga aattatgagc cagaaattaa aagtcgtcac tattggtggc gggagcagct
     2761 ataccccgga gttactggaa ggatttatta agcgttatca cgaattgccg gtcagcgaat
     2821 tatggctggt ggatgtcgaa ggtggtaaag cgaaactgga tattattttt gatctctgcc
     2881 aacggatgat tgataacgct ggcgtcccga tgaagcttta taaaacgctg gatcgccgcg
     2941 aagcattgaa agatgctgat ttcgttacta cccaactgcg cgttggccaa ttaccggcgc
     3001 gcgaactgga tgaacgtatt ccattaagtc atggttatct tggtcaggaa actaacggtg
     3061 cgggcggtct gtttaaaggt ctgcgtacca ttccggtgat ttttgacatc gtaaaagatg
     3121 tcgaagaact atgtccgaat gcatgggtga ttaacttcac taacccggcg ggaatggtca
     3181 ctgaagccgt ttatcgtcat accggattta aacgctttat cggcgtgtgt aatattccga
     3241 tcggcatgaa gatgtttatt cgcgatgttc tgatgctgaa agacagcgat gatttatcta
     3301 tcgatctgtt cggcctcaac catatggtgt tcattaagga tgtgctggta aatggcaaat
     3361 cacgctttgc cgaattgctt gatggtgtgg catccgggca gttaaaagca tctggcgtta
     3421 aaaatatttt cgatctgcca tttagcgaag gcttaattcg ttctatgaat ctgttgccgt
     3481 gttcttattt gctttattac ttcaagcaaa aagagatgct ggctattgaa atgggcgaat
     3541 actacaaagg cggcgcacga gcgcaggtag tacagaaagt cgagaaacaa ctttttgagc
     3601 tatataaaaa cccggagttg aaagttaagc cgaaagaact ggaacagcgc ggtggggctt
     3661 attactctga tgcagcgtgc gaagtgatca acgctatcta caacgacaag caagctgaac
     3721 attacgttaa tatcccgcat catgggcata ttgataatat tccggcagac tgggcggtag
     3781 aaatgacctg tacgctgggg cgcgatggcg cgacgccaca tccgcgcatt acgcatttcg
     3841 atgataaagt gatggggctg attcacacca ttaaaggctt cgagattgct gccagcaacg
     3901 ccgcacttag cggagagttt aacgatgtgt tactggccct aaaccttagt ccgttggtgc
     3961 attccgatcg cgatgctgag ctgctggcac gcgagatgat tctggcgcac gagaaatggt
     4021 tgccaaattt tgccgactgc atcgcagagc ttaaaaaagc acattaaccg aggctgatta
     4081 tggaacgctt actgattgtt aatgccgatg attttggctt aagcaaaggt cagaactacg
     4141 gcattatcga ggcctgtcgc aatgggattg tcacatcgac gacggcgctg gtgaatgggc
     4201 aggctattga ccatgcggtg cagttgagtc ttgatgaacc gagtctggcc atagggatgc
     4261 actttgtcct tactatgggc aagccactga cagctatgcc ggggttaact cgcgatggtg
     4321 tgctgggaaa atggatctgg cagttggcag aagaagatgc tttaccgctg gaagaaatta
     4381 ctcaggagct tgccagtcag tatttgcgtt tcattgagtt atttggacgc aaacctacgc
     4441 atcttgatag ccatcatcat gtgcatatgt tcccgcagat tttcccgatt gtggcaaagt
     4501 ttgcggctga agaggggatt gcgttgcgca tcgaccgtca gccgctatct aacgatggtg
     4561 atttaccggc taatctgcgc agttcgcagg gattcagtag cgcgttttat ggtgaagaga
     4621 ttagcgagac actgttccta caagtgcttg atgactccag tcatcgcggc gagcgctctc
     4681 tggaagtgat gtgccacccg gcgtttatcg ataatacgat tcgccagagt gcttattgct
     4741 tcccgcggtt aacggagctg gatgtgttga catcagcgtc gttgaaaggt gctatcgctc
     4801 agcgtggtta tcggttgggg tgttatcggg atgtgtaagt gaggatgttt gccagatgcg
     4861 gcgtgaacct tgcatccggc gattaattcc tacaaaggcg gcgtaactgc gccgcttgag
     4921 actgctgaca aacgcaaaac tgcctgatgc gcttcgctta tcaggcctac gtttttcctg
     4981 caatatattg aatttgcaca gttttgtagg ccggataagg cgttcacgcc gcatccgaca
     5041 taaacaaagc gcactatgta aatcatttga ggcggcgcaa ctgcgccgcc tcccatcagg
     5101 caggaatttt gtcaatctta ggaatacgtg accacacgcg gtgtgctgcc atcagcgtta
     5161 acagttcatc cataaaacta ccgtcggcgc tgtcagcttc cacaatccct tcttcaccct
     5221 ggtcagcgac cttgattgtt gctttaaact tgcgcgcgtc tcctgccagc gcaatcggtt
     5281 taaggtgttt gtaggcttcc atcaggtagt agttggcatc gccgttgtca gcgatatccg
     5341 cgatattgcc gcaagggaca ataaccgcat cgaccgtcag cgaaggtgca ccggcaaagg
     5401 tagcggctat aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga
     5461 gcagtttggc atgaacgcct ttggccttca gcgccttgag aatggccaga aggtctgccg
     5521 atctcacttc atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa
     5581 tggcgtacaa acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggcggggtga
     5641 tattcagctg gtcgtccgtc agctcgatac cgagattttt cgccaccgcc tgggccagag
     5701 tgagatcaat atgtgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt
     5761 tgcttaactc aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta
     5821 gccagaacag acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt
     5881 tattgccttc cacgcgctcc tggtatgatt caaaaccgcc gcgtttcggc cccggcggtg
     5941 tttcgcgcgg ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga
     6001 tccccatacg atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa
     6061 tcggaatctc atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga
     6121 acaaacgtcc ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg
     6181 gatggaatgc tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca
     6241 tcttgccgac acgctgaacg ggcaccagtt cttccgggat aagtttagtt ggatcaagaa
     6301 ggtcgaagtc gaacttaaat tcatcttcct caggaatcaa ctggaagccc agttcgtatt
     6361 ccggaaaatc gcctgcttcg atggcttccc acaactcgcg gcggtggaag tccgggtcac
     6421 gtccggttag tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc
     6481 agtggaaacg tacgaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac
     6541 cgaagccttc catggtgcgg taactgcgcg ggatcccgcg atccgacatc gcccacatca
     6601 cgttgtgcag agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcgcttt
     6661 gcccttgtgg aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt
     6721 tatgcgcatc ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct
     6781 cttcggtata gaacttggtg gcaaagccac ggatatcacg cacggtatcg gcagagccag
     6841 caccaccctg aacggtagag aaacgtacaa atactggggt gattttgttc ggatctgaga
     6901 ggaaatctgc tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg
     6961 ctgatccgcg tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgagtgattt
     7021 tctcgcgcag aataaaatct tccagcagag ttggaccacg gctaccggca cgcagtgagt
     7081 tttgatcgtc ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt
     7141 tgcgtacgtc ttccagagaa ttaagttttt cgttacgcgt atcaggggct ttcaggctcc
     7201 ctggggcggt aggttgtgca ccaggcggtg ttggttcagc cgctggacga tgagatccgt
     7261 cttcaggtgc cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact
     7321 ggtgctgatg tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaaattatt
     7381 actgaaaggg ccgctgtttt ggtcaaaaaa ccctctaact atagaccatc agccagatcc
     7441 cgcttcggag actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg
     7501 ggctaaatcg gctaaactac agtttttctg atttttaata tgcgtttagt gaagccagta
     7561 atgaagaaac cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa
     7621 cccgcgccgc cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt
     7681 ggcaaatatg ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact
     7741 gtgcctgaat ccgcccagcg ttgggcaaat cagattcgtc aggaaaatga ggtcgaagaa
     7801 taaaaaaaca cctgtcggga tgacaggcgt ttaacgtaac aaatcaggat taacggtgtg
     7861 ccagttcggc gtcgtcttcg ctatccagaa tagctttatc ggtttgcttc agccactggc
     7921 tggtcagcgt gccagcggtc atcgagccac taacgtttag cgccgtacga cccatgtcga
     7981 taagcggttc aacggagatt aacaatgcca ccagcgttac cggcaggccc atcgcaggca
     8041 gcacaatcag tgcggcgaaa gttgcaccac caccgacacc ggcaacgcct gcggaactaa
     8101 cggtaacaat accgaccagc gtcgcaatcc acatcgggtc catcgggtta atgccaaccg
     8161 taggcgcaac catcaccgcc agcatcgccg gatacaaacc ggcacagccg ttctgaccaa
     8221 tggttgcacc gaaagaggcg gcgaaactgg cgatggattc aggaacaccg agtcgacgcg
     8281 tttgtgcttc cacattcagt gggatagacg cagcactgga acggctggta aaggcaaacg
     8341 tcagtactgg ccatacctta cggaagtact tcagcgggct cacgccatta atacccagca
     8401 gaaggccatg cactgcaaac ataatcagaa gaccgaggta ggacgcgaca acgaaacttc
     8461 ccagtttgat gatgtcttgc aggttagaac ctgcaaccac tttggtcatc agcgccagaa
     8521 cgccgtaagg ggtcagctgc atgaccaggc gaaccagttt catcacccag ctttgcaggg
     8581 tatcgatagc ggttaagacg cgttcacctt tcggcgcatc atccttcagc agcttcagcg
     8641 cagctacgcc gaggaatgcg gcaaaaatta ccacgctgat aattgacgtc ggattggctc
     8701 cggtcaaatc ggcaaacggg tttttcggga taaaggacaa gaccagctgc ggaacgctca
     8761 ggtcagagac tttaccaaca tagttacttt cgatggcgtt caaacgtgca gtttctgcac
     8821 caccctgaac cagaccttca gccgtcaaac caaacaggtt ggtgaccagc acaccgacca
     8881 gcgccgcaat cagtgtggta aacaaaagcg ttccgatggt cagaaaactg attttgccta
     8941 actgtgatgc gttatgcaga cgggcaaccg cgctcagaat agaggcaaag actaacggca
     9001 taacgatcat ttgcaggagt tgaacatagc cgttaccaac aatgttaaac cactgtacag
     9061 aatctttaag tacctggctg tcagaaccat aaatggtatg cagggcgagg ccaaaaacca
     9121 cacccatcac cagacccacc agcacttttt tcgccagact ccactgttta tggcgggtct
     9181 gagccagcgc aaacagcagt acaacgaaca ccacgatgtt cgcaattaat ggaaagttca
     9241 tccccgttct cctgatctta ttatttctat cactgcgtag cagtcatagt gttggcgcaa
     9301 gattaacaga actgtgagct ggctcttata ttcaaatgga attgcttatt ccagaaaggt
     9361 gatttttgcc tttttagtat tcaacctgat gcttaataag ccgatgaaac tggatctgca
     9421 aggtattgat catttgcgat gaccaacgga cagcgctgtt ttcgggtaat gaatggggca
     9481 tccataccga ccagacaaat aatgccatgc agataaaacg ttccagttgg ttgccctttt
     9541 ggggaaccag gataggcaag cggaaacgcc agcggcatgg ccagagcagg ggaacaccgg
     9601 cgggtgtcag catatcggca agtatgtggc tcaaataacc cagcaccatt ccttgtagcg
     9661 catcagccgg aatgaaccag ctttcaggaa ccttaaggta aaaggttgcc agcagcgcaa
     9721 ataccgccag cagactgtgg gtaaaaccac gatgcccaaa agcgcgggcg attggttttg
     9781 atatccactt taatcgctgc ccaagaaacg actttgggtg atcgatgtcc ggtaacaaac
     9841 acgtcaggat tgcggaaggg acaatatgcc accagtcacc ctgtgccagc acgggcgtca
     9901 gctcggcatt tttggcaaat accgcacaag caatagaaaa gagaaggtga ccttccgccg
     9961 tcatgataaa actcactaaa ctgtcgatac gtacagtata gggtttttat acagtgcacg
    10021 gaagtggtga cggtgtaact ctttctctta aattggcgat taatccgtca gttgaatgca
    10081 aattcagata aagaaccagg ggcagtgtga tcactgcccc ctggtcatta accgagaagg
    10141 tcttttgcgg tgagttctgt cagcgatgag agtttgacgt ttgctaatac aaaacgtgga
    10201 tcattttgcg cttctggcgc aggaacgacg atggaacgca tgcgggctgc tttagaggcg
    10261 atcatgccat ttaccgaatc ttccagtgcc acgcaggtca gagggtcaac ccccagtttt
    10321 gctgcgcagt cgatatatac ttgcggatgc ggcttgctgt aaggcaattt ttcagccgag
    10381 gcgagggcat cgaaactgtc gcgtaaatca aacatggtca atactttttc cagcatatgc
    10441 agcggtgacg cggaggccag ccccaccaat aaaccttgtt ctttgcataa cgcaacggct
    10501 tcacgcacac ctggtaataa tggacgcgtc tcttcaacca gtgaaatggc acgagcaata
    10561 acttgttcta ctacttcctg acggcttggc ccattccatg gttgccgggc gtaccaaaga
    10621 tcgaccacca tatcgatgcg taaacctaag gtgtccggca gctcgttacg acgggagata
    10681 tccaccccca ggcttgccat cacatccagt tcggctcgat cccataaagg ttctgagtcg
    10741 ataagtaatc catccatatc aaaaattgca gcaagaatct gacgcggggt tgacattaca
    10801 acctctcctt tagagcatta tatgtaaggt attaagccgt gcttatgatt aagcgcctat
    10861 catatcgcct ttaaagaatg ggcgaaaaca gatatctaca ggtaaactaa gtgaaacgac
    10921 gcgtcttaat caaggagaac tcatgacgta tcaacaagct ggacgcattg ctgttttgaa
    10981 aaggattttg ggttgggtga tttttatacc tgcattgatc tctacattga tttctttgct
    11041 taaatttatg aatacccggc aggaaaacca ggaaggcatt aatgcggtca tgctcgactt
    11101 tactcatgtc atgatcgata tgatgcaggc gaatacgcct tttttgaatc tgttctggta
    11161 taactccccg acgcccaatt ttaatggtgg cgtgaacgtt atgttctggg tgattttctt
    11221 cctgattttt gtcggactgg cgttgcagga ttccggtgcc cggatgagtc gccaggcgcg
    11281 cttcttgcgg gaaggtgttg aagatcaact cattctggaa aaagccaaag gggaagaggg
    11341 gctaacgcgt gaacaaattg agtcacgtat tgttgtgccg caccatacta ttttcctgca
    11401 gtttttctcg ctgtatatcc tgccggttat ttgtattgct gcaggttatg tgttcttttc
    11461 tctgctgggg tttatttaat caaaaggccg cacggagcgg ccattatcct caccccatat
    11521 tatgctgcta ataatctatc cagtgactgc tgagcaataa ccaaatgctg accgccaaat
    11581 aaccttgcac gatttaacag cgtgtagagt tggtaaaccg gttgacgttc aaggaaatcg
    11641 gcaggtagcg gggatactga ctgatagccg tcatagattt gtggcggctg ttcagtatgc
    11701 agcggtaaca tcgccaggtc gcactctcgg tcaccccagt agcaggccgg gtcgaaaatg
    11761 tacgggccat ccggacccag cgcacagttg ccggaccata aatcgccgtg caacagagaa
    11821 ggctgcggtt gatgcgaggc cagacgctgc tgaatatgct cgacgatggc atcgatattg
    11881 ccgaaagcga tccctttttc tgccgccagt tccagctgcc agccaatccg ttgttcagca
    11941 aaaaaagttg accagcgacg ttgccaggtg ttgggctggg gagttgtgga gagcgcgtta
    12001 tcgaaatcga ggccaaattg cggttggtcg ctccattgat gtaaacgcgc aatttgctga
    12061 ccaagaataa atgcgctatg cgcatccagc gggcgaggtg ggagataatc catcaccaga
    12121 aaactgtagt cacggtcagc gccaactgcc caaaccttag gcacggtgac ggttttacta
    12181 cgcgacagta actccagttg gtcggcttcg gcggtaaaac cgggaagcag ttccctttca
    12241 tcacatttga cgaaaaagtc atgtcctgca tagcgcaaat gccatgcggc atggacttct
    12301 ccgccaggca gttcattacg cagttcgatt tcgccttcac ctaactgctc gctcaaaaga
    12361 cgactgattg cctgccacat gatgtctctc ccatgttgtc tgccagatca taaggttagc
    12421 gcaagaatgc ggtgtaaaaa cacgagctaa cgcacaccac agactgatta ttgcccaaaa
    12481 accatttatt ttctgttgct attccattcc tcccaggtcg tgatggctat ttcaggatct
    12541 ttaccggttg cactttgggt aagccccgaa acaagttctc taatttcttc ttcactttgg
    12601 gtactgataa gtccaaaagt attagtcccc agctcatgga tatttccatc atcatccgtc
    12661 atggtgagca aaaaaccgtc gcgagtcagg taattattca actcgttaat ttcagtcaat
    12721 gtatcttcgt gcaacatgac ggttatgaca taacggacaa gatcgccact ggccatagtt
    12781 cacccctttg ttatcacatg acttttgagc atagtcggag aaacgcgttg ccgacaggtt
    12841 ggtggtgatt cccccaatgc tgggggaatg tttcagttag cgggaaaggt aagcgtaaat
    12901 tttttgtgta tcgtcatggg agcacaggcg tgttccctga ttgagtgtgg ctgcactccc
    12961 cgcagctacg ccaaaacgga ccatctcttc aagagaggca ttttctgcca gtttcagtgt
    13021 catcgcgccg accatgctgt caccagcgcc aacggtgctc tggcttttca ccggtggtgg
    13081 cacaacctga atgcagtttt cactatcaac gcccagcgcc ccttgtggac ccagggaaac
    13141 gacaactcgt ttggccttgc cgctattaac gatttcctgc gcggctttgc ggacatcgtc
    13201 tggctgggtg agttcgcgat tcaccagcgc actgagttct ttttggttag gcttaaccaa
    13261 ctcgatgtta ccaatcgcca gtgctgcact taacgcttcg ccagaactgt cgacgataca
    13321 gcggatccct tggttttgcg cagcggaaat cagttgggtt aatttttcca gcttcacacc
    13381 tggcggcagg cttccgctta tgaccaggat agcaccggat tcaatttcca gaacttgctc
    13441 ttcaagctgg cgaaactcat cttcatttaa tgccgcgcct ggcatgacaa aacgatactg
    13501 ctcaccgctt gcttccacat gtacgtgtaa attctgccgg gtccagtctt tggcttctac
    13561 agtagcgacg gggacatttt catctgccaa cagtgaaacc agatgttcgc cggtcgcacc
    13621 tcccaccggg aagatcgctg tagcactgcc tccaagatgg gcaatggcgc gggcgacgtt
    13681 gatgccgccg cctccgggtt cgaataccgg tgcggtacag cgcagctttc cttcgggata
    13741 aatttgcggg gtaattgttg cgctatcgag agagggcgca agtgtcaacg tatagatacg
    13801 taccatcatt tcctcctata ggctgatttc agtctggcac cgaatcagcg gaaagtgaag
    13861 aaattaacaa tatgatttat tagagcttta tttaaaattt tgcagataaa tatatataaa
    13921 taaaaatttg ttttcgccac atgcttattc aaaaaatgaa ataagaatac attgctaagt
    13981 tattcgcgcc tttttataat tcgttacctt tccagggacg ctaatctttg atccttaaga
    14041 aagtttccgg gaccgcgaag gtctcttttt ttgttgtacg gactcttgat aaatgaagct
    14101 tttgaagaca gttcctgcca tcgttatgtt ggcggggggc atgtttgcgt cactgaatgc
    14161 agctgccgat gattccgttt ttactgtcat ggacgatcct gccagcgcta aaaaaccttt
    14221 cgaaggtaat ctgaacgcag gatatcttgc acaatcaggc aatacgaaaa gctcctcact
    14281 tacggctgac accaccatga cctggtatgg acaaaccact gcctggtcgc tgtgggggaa
    14341 cgccagtaat acttcttcta atgatgagcg ttcttcagaa aaatatgcgg cgggcgggcg
    14401 tagccgtttt aatttaactg attatgacta tttatttggg caggcaagct ggctgacaga
    14461 ccgttataac ggctatcgag agcgcgatgt gttgaccgcg ggttatggtc gccagttttt
    14521 gaatggcccg gtacacagtt tccgctttga attcggtcca ggtgtgcgtt acgacaaata
    14581 taccgataat gccagtgaaa cccagccgct gggttatgcc tccggggctt atgcatggca
    14641 gttgactgac aacgctaaat ttacgcaggg tgtttccgtc tttggcgcgg aagacacaac
    14701 gctgaactct gaaagcgcat taaatgttgc tattaatgaa cattttggac tgaaggtggc
    14761 ttacaacgta acatggaact ctgaacctcc agagtctgca ccggaacata ccgatcgtcg
    14821 gacaacctta tcgctgggct acagcatgta aaattatctg ccggaccgtg ttatgcggtc
    14881 tggctgtctt cgtttgtcac tgctcagccc caaatcattg ctgcccaacg tagcaacaac
    14941 atagcgccac agattgccag tagcaccacc accatcttcc agtgtttttt atcaaagcgt
    15001 ttagtcggca taccagtgtc ctgcatatct cttgtgcgtg ataacggagt gtaacaaaag
    15061 ggacaggcat caagggattt tttgttatta cagccgtcgg gtggcgttgt acacctgatc
    15121 actgtttttg cggtttatga aaaaaataaa attatgtatc gatatataaa tatctggtta
    15181 taaatacgtc tttagttcat cagcactaaa actcaggctt tggctgat
//
LOCUS       DAZGHF010000115        15198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGHF010000115 DAZGHF010000000
VERSION     DAZGHF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.116"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(277..352)
                     /locus_tag="ACHK3A_003732"
     tRNA            complement(277..352)
                     /locus_tag="ACHK3A_003732"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(317..319),aa:Phe,seq:gaa)
     gene            complement(458..1165)
                     /gene="yqgA"
                     /locus_tag="ACHK3A_003733"
     CDS             complement(458..1165)
                     /gene="yqgA"
                     /locus_tag="ACHK3A_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO8741763.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1318..1395
                     /locus_tag="ACHK3A_003734"
     CDS             1318..1395
                     /locus_tag="ACHK3A_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741764.1"
                     /translation="MHATSLHSLAYRRFARNSSLFLLMR"
     gene            1558..3693
                     /gene="speC"
                     /locus_tag="ACHK3A_003735"
     CDS             1558..3693
                     /gene="speC"
                     /locus_tag="ACHK3A_003735"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO8741765.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAAEQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLFGYVLK"
     gene            complement(3743..4999)
                     /gene="nupG"
                     /locus_tag="ACHK3A_003736"
     CDS             complement(3743..4999)
                     /gene="nupG"
                     /locus_tag="ACHK3A_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO8741766.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5201..6280)
                     /gene="mltC"
                     /locus_tag="ACHK3A_003737"
     CDS             complement(5201..6280)
                     /gene="mltC"
                     /locus_tag="ACHK3A_003737"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO8741767.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6345..6620)
                     /gene="yggX"
                     /locus_tag="ACHK3A_003738"
     CDS             complement(6345..6620)
                     /gene="yggX"
                     /locus_tag="ACHK3A_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO8741768.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6648..7700)
                     /gene="mutY"
                     /locus_tag="ACHK3A_003739"
     CDS             complement(6648..7700)
                     /gene="mutY"
                     /locus_tag="ACHK3A_003739"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO8741769.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7861..8580
                     /gene="trmB"
                     /locus_tag="ACHK3A_003740"
     CDS             7861..8580
                     /gene="trmB"
                     /locus_tag="ACHK3A_003740"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO8741770.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8580..8906
                     /gene="yggL"
                     /locus_tag="ACHK3A_003741"
     CDS             8580..8906
                     /gene="yggL"
                     /locus_tag="ACHK3A_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO8741771.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            9090..9809
                     /gene="yggN"
                     /locus_tag="ACHK3A_003742"
     CDS             9090..9809
                     /gene="yggN"
                     /locus_tag="ACHK3A_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO8741772.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9985..11031
                     /gene="ansB"
                     /locus_tag="ACHK3A_003743"
     CDS             9985..11031
                     /gene="ansB"
                     /locus_tag="ACHK3A_003743"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO8741773.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDISKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11148..12155
                     /gene="yggM"
                     /locus_tag="ACHK3A_003744"
     CDS             11148..12155
                     /gene="yggM"
                     /locus_tag="ACHK3A_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO8741774.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPIKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSADCYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQTNERWKKDTEQLRRNGVI
                     K"
     gene            complement(12211..13512)
                     /locus_tag="ACHK3A_003745"
     CDS             complement(12211..13512)
                     /locus_tag="ACHK3A_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO8741775.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLIALMMITYIPQISLFIPQLLGLM"
     gene            complement(13512..14015)
                     /locus_tag="ACHK3A_003746"
     CDS             complement(13512..14015)
                     /locus_tag="ACHK3A_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO8741776.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEVS"
     gene            complement(14060..15046)
                     /locus_tag="ACHK3A_003747"
                     /pseudo
     CDS             complement(14060..15046)
                     /locus_tag="ACHK3A_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         4027 a         3643 c         3853 g         3675 t
ORIGIN      
        1 cagacactgg aagatgccct gagtttgtat acagatgagc gcacagtgag caagatttgc
       61 aaagggggga ataaatcttg acaggctgaa tcagcaatgt aagcatacag tgttaacgag
      121 aacagggcgt ctctgcaaaa gacaccctgt ttgaggatac gataccgaac tcaatcagtg
      181 taaccacaac ttacacaaac ggagaccctt tgatgccgca tcctgtatta accgcccgtc
      241 aaggatctca tcccttaacc agcgtgttct ggtatatggt gcccggaggc ggaatcgaac
      301 caccgacacg gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc
      361 gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc
      421 acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg
      481 cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt
      541 taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt
      601 ctgccatcat cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta
      661 actggatgat cagtaatggg atactaatta ccgacaccgc aatacccaac gagcaggcga
      721 agatcatcgc cgtaaagaaa tcgagaaatg acttggcgat taaaatactc ggatcgccgg
      781 tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa
      841 tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag tggcgaaaca
      901 gattttgtgc tttggtgacc gcggtattga cgcctttttc cagcagacaa atttcgccga
      961 ttaatgcacc gagtaaggtt gctaaaacca tcgccggaag gttggcgcat ttcaccacca
     1021 gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac
     1081 gttccggtaa acgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag
     1141 cgttgataaa agggccgatg accacaagag ttcctgttaa ctgtattgtc tgattttctt
     1201 attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgct
     1261 ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg
     1321 cacgccacct ccttacattc tctcgcttat cgccgtttcg cgcgaaactc ctcccttttt
     1381 ctgctcatgc ggtgaagtta acgcacgctc actgcaggac aacagtaaaa tcagagcgtt
     1441 tctgctttta ctgatgtctg gcggtcggag ctggtgacca gtttgaccca catctcatgg
     1501 ggcaggtttg tccatcctgt ccggtactct tacttccccg aaacgggttt tgcgcttatg
     1561 aaatcaatga atattgccgc cagtagtgaa ctggtatccc gactttctac tcatcgccgc
     1621 gtggtggcgt tgggagatac tgattttacg gacgtcgcgg cagtcgtcat taccgctgcg
     1681 gatagtcgca gtggcattct tgcgttgctt aagcgcaccg gttttcatct accggtgttt
     1741 ttgtattccg aacatgttgt tgaattacct gcgggcgtta cggcggtaat caacggcaac
     1801 gagcagcagt ggctggagct ggaatccgca gcctgtcagt atgaagagaa tttgctgcca
     1861 ccgttttatg acacgctgac gcagtacgtt gagatgggca acagcacctt tgcttgccct
     1921 ggacatcaac atggtgcgtt ttttaaaaag catcctgccg gacgccattt ttacgatttc
     1981 tttggtgaga acgtctttcg cgccgatatg tgtaacgctg acgtaaaatt gggcgatctg
     2041 cttattcatg aaggttcggc gaaggatgca cagaaattcg cagccaaagt ctttcatgcc
     2101 gataaaacct attttgtgct gaacggcaca tcggcagcga ataaagtggt gacgaatgcg
     2161 ctgttaacgc gtggcgatct ggtgctcttc gaccgtaaca accataagtc gaatcatcat
     2221 ggcgcgttga ttcaggcggg ggcgacgccg gtctatctgg aagcttcacg caacccgttt
     2281 ggtttcattg gtggtattga tgcgcactgt tttaatgaag agtatctgcg ccagcaaatt
     2341 cgcgacgttg cgccagaaaa agcagacctg ccgcgtccgt ttcgtctggc gattattcag
     2401 ctgggaacct atgacggcac tgtctataac gcccgtcagg tgatcgatac cgttgggcat
     2461 ctgtgtgatt acattctgtt tgattccgcg tgggtcggtt acgaacagtt tatcccgatg
     2521 atggcggata gctcgccgct gctgttagaa cttaacgaaa acgatccggg gatctttgtg
     2581 acgcaatcgg tgcataaaca gcaggcggga ttctcacaga cgtcgcagat ccataaaaaa
     2641 gataaccata ttcgcgggca ggcgcgtttt tgcccgcata agcggttaaa taatgccttt
     2701 atgctccatg cttctaccag cccgttctat ccgctgtttg ccgcgctgga cgttaacgcc
     2761 aaaattcatg aaggggagag tgggcgtcgg ctgtgggcgg agtgcgttgc gttggggatt
     2821 gaagcccgga aggcgattct tgcgcgctgt aagctgttcc gcccgtttat tccacccgtt
     2881 gttgatggca aattgtggca ggattatccg acgtcagtgt tagccagcga ccgtcgtttt
     2941 ttcagttttg agccgggggc gaagtggcac ggctttgaag gatatgccgc ggagcagtat
     3001 tttgtcgatc cgtgcaagct gttactgacc acaccaggta ttgatgctga aaccggtgaa
     3061 tatagcgact ttggcgttcc ggcgacgatt ctggcgcatt atctgcgtga gaacggtatt
     3121 gtgccggaga agtgcgatct caactccatt ctgttcttat taacgccggc ggaaagccac
     3181 gagaagctgg cacaactggt ggcgatgctg gcgcagtttg aacagcatat tgaggatgac
     3241 tcgccgctgg cggaggtgtt gccgagcgtt tataacaaat atccggtgcg ctatcgcgac
     3301 tacactttgc gccagttgtg tcaggagatg cacgatctgt atgtcagttt cgacgtcaaa
     3361 gacctacaaa aagcgatgtt ccgccagcag agtttcccgt cagtggtgat gaacccccag
     3421 gatgcgcata gcgcttatat tcgcggtgaa gtggagttgg tgcggattcg tgatgccgaa
     3481 gggcgaattg cggcagaagg ggcgttgcct tatccaccag gcgtgctttg cgtggtaccc
     3541 ggagaagtct ggggtggggc ggttcagcgt tatttccttg cgctggaaga aggggtgaat
     3601 ttattgccgg gattttcgcc ggagctgcaa ggtgtttata gcgaaaccga tgcggatggc
     3661 atgaaacggt tgttcggtta tgtgttgaag taagaataaa aaaaacgggt caccttctgg
     3721 cgacccgttt ttctttgcgt aattagtggc taaccgtctg tgtgcctgtc gggacacgaa
     3781 cgtgtttata tttgaacatc gccatgaacg cgaaggccag aaccacggag taaccagcga
     3841 aaatcagcca tacggtctgc cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt
     3901 taccgctcac gatgccgccg aggatacagc cgaagccgtt agtcatcatc aggaacatcc
     3961 cctgcgcact ggcgcgaatt gccgggctaa cttctttttc gacaaacacc gaaccagaga
     4021 tgttgaagaa gtcgaatgcg caaccgtaaa cgatcatcga cagtaccagc agtacagtac
     4081 cgaacggagt cgggtcgccg taagcaaaca gcgcaaaacg caggatccac gccacaatac
     4141 tgatcatcat tacgttctta ataccgtagc ggcttaagaa gaacgggatg gtcagaatga
     4201 acagggtttc agagatctgc gaaatcgaca tgatgattga cgcatgctgc acgatgaagc
     4261 tgctggcaaa catcggatct ttgtcgaagc tgtgcaggaa ggtgttaccg aacatgttgg
     4321 taatctgcag ttccgcgccc agcagcattg agaagatgaa gaagattgcc atacgcttgt
     4381 ttttaaacag cgcgaatgca tcgaggccca gcagggttgt ccagctctga ttcgcttgct
     4441 gtttagcaac cggaatatgc ggcagagtca gggtaaacag aaccagaatg gcggaaagtg
     4501 ctgcgccaat atacagctgc atgtggctta attcgaagcc agacaggctc accacccaca
     4561 ttgccatgat aaagccgatg gtgccccaga tacggattgg cgggaagtca gtaacgatat
     4621 ccatcccggc attttgcagg cgatagtaag agatggtgtt gattaaccca agcgttggca
     4681 tataagcaaa cgagttaatc aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg
     4741 ccgccatgaa cagcgtgata gcgccaatgg tgtggcaaat ggcatatacc catttcgcac
     4801 ttaaccattt gtcggccaca atccccagca gcgcaggcat aaataccgcg gcgataccca
     4861 gtgagctata aactgcgcca atagaagcac cgtcaaactt cagggtaaca aacatatagg
     4921 agccgagggt cgtcagccaa cttccccaca gacagaactg cagaaaagag aggattttca
     4981 gctgcagctt aagattcatg ttaatttcct cacatcgtga tgcggatgag tgtttgaaaa
     5041 agcacatttg accgtgcgag atgatgggat tctatcaact tcccggactg gttttttgtt
     5101 acctgtggca aataattgca aacattccca atttgcaaaa cgaaattgtg atgtggataa
     5161 catttttacc cctgagcatc gtcaggggcg gttagtggaa ttatcggcgg cggtaggatt
     5221 tttgcgcggt attcacttta taaagataac ggcgagattc cgcagaggga tggcgggtcg
     5281 tcagcgtttg ataaacatcg cccggcgtca tggtgttaat aatattggcc gcctgaatct
     5341 tatcattcga aaagactcgc agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg
     5401 cataacgccg ggatgtaggg ttatcaattc cgccgagata aacgttgttc agcatcgcca
     5461 gatacgcggt gccggtatca atattgctgg caggatcaaa caagaaactg cggctcggcg
     5521 tgccggattt cccctgcgaa cggaacacat ctttcccggc agtatgttgt accacctgca
     5581 ttaaccccag cgcatcggaa cggctgaccg catacgggtt aaaggatgac tcggtctgca
     5641 taatcgccag aatcagcgac tcatcaacgc catatttccg tgacgcctgg cggaccatgc
     5701 cgagatattt gtgcgcacgt ttatcaaggt ggttcggcac catgttaatg gtgacgctgt
     5761 agatgatacg cagcccgttg ctgcggctct tcagacggtt tttcagcaga taatccgcga
     5821 agttgctcgc gcgaccttcc cagcgaatcg gctgcccggt gttgtccacc acctgaccgt
     5881 aaaggaaagg ttctttcgaa atcgtaatat catcaacgtc ggaatagaga tcgaccgaac
     5941 tcggatcgtc ccccatcagc aacgttttga taattgcccg gcgcagatgc gcggcaggtt
     6001 ctgtcccggc gatggtttcg atagtaatcg taccgtcatc gaagttgatg tggctgcggg
     6061 tctgatattg atcggtgtat ttcacgtagt ccttaggacc ggcgatcacc acctctttga
     6121 agccccagat gttctcaata ttgtgggcaa attgccccat cagaatatca aaaccgttgg
     6181 tatctttgac ccaggcttcg ttataggtat cgcctttttt ggtcgtcgaa caggagatga
     6241 gcaacggcgc aatcaaagcc agcgcgagat attttttcat cattccgggt gcgtgttgtg
     6301 tttgttttta tgcaagttgc cggaggcgtg ctccggcact gtttttattt tttatcttcc
     6361 ggcgtatagc cctcaatatg cacctcttta ccctcgaaca ggaagttgac catctcctgt
     6421 tccagcagct tgcggtgctc ggcattcatc atgttgagtt tcttttcatt aatcagcatg
     6481 gtttgtttgt gctgccactg cgcccaggct tctttggaga tctcgttata gatgcgtttt
     6541 cccagctcgc cggggtacag ctgaaaatcc tgaccttctg cttcacgttg caggaaagta
     6601 caaaaaatcg ttctgctcat aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg
     6661 ccagtgcgta actgctgtaa caaacgctcc acgggagccg ccaggccaac tgacggcggt
     6721 tgcgctaagt tataccagag cgcattgcct tcatccatgc agccggtgaa tgacgacacg
     6781 ggaagccaca taggcacaat atctaagtgg aaatggctga aggtatgccg aaacgcggtc
     6841 agctgcgtca ggttatcggc agcaatctgc cgttgcgcca gccactgccg caaactttct
     6901 tcgtcggcaa actgcgggaa acagtataaa ccgccccaca atccgctcgg cggacgctgc
     6961 gccagcaata cttcatcttc gtgctgtaac aggagaaagt agccggtgcg ctccggcagc
     7021 gtctgtttcg gttttttgcc cggataaagc gaccagctat tgttggtggc ggcaatacat
     7081 ccgttttgta gcggacagag cgagcatttc ggcttcgagc gcgtgcaaat catcgcaccc
     7141 aaatccatca tcgcctgatt aaaccgctcc acgccgaccg cgggcgtcac ctgttcgctc
     7201 aaactccaca gtttattctc gacctctttt ttcccaggcc agccgcttac agcatagcag
     7261 cgcgccagca cccgtttgac gttaccgtcg agaatcggaa agtgcttacc cagagaaagc
     7321 gagagaattg cgcctgcggt ggaacgcccg acgcccggta acgccgcgac ttcttcaaag
     7381 gtttccggga atttaccgcc gtgtaaggtc gccacttgtt gtgccgcctt atgcagattg
     7441 cgcgcgcggg cgtaatagcc aagcccggtc cacaagtgga gaacttcgtc gagcggcgca
     7501 ttggcgagat cggtcaccgt cgggaagcgc gccataaagc gttcaaaata ggggataacg
     7561 gtcgcaacct gagtttgttg caacatcact tctgagagcc atactttgta gggcgtcttg
     7621 tcaatttgcc agggcagcgt tttccgcccg tatttatcgt accagtccag aacctgggct
     7681 gaaaattgcg acgcttgcat ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca
     7741 cagcgacggc agggtgtaaa ccggaacttt ccgcagccac ggccttatca tgcttgcatc
     7801 cggcaactaa ctttggataa tgcccgtttt cagaacactt tcacaagcga ctaaatcttt
     7861 atgaaaaacg acgtcatctc accggaattt gatgaaaacg gccgcccact gcgccgtatc
     7921 cgtagttttg tgcgccgcca ggggcgactg accaaaggcc aggaacatgc gctggaaaac
     7981 tactggccgg tgatgggcgt tgagttcagc gaagatatgc tggatttccc cgcgcttttt
     8041 ggccgagaag cgccggtgac gcttgagatt ggtttcggca tgggggcgtc gctggtggca
     8101 atggctaaag atcgccccga gcaggacttc ctcggcattg aagtgcattc accgggcgtt
     8161 ggtgcgtgcc tggcttctgc gcatgaagag ggcttaagca acctgcgcgt gatgtgtcac
     8221 gatgcggttg aagtgctgca taaaatgatt cctgacaatt cattgcgcat ggtgcagctc
     8281 tttttccctg acccgtggca caaagcgcgc cataataaac gccgtatcgt tcaggtgccg
     8341 tttgccgaac tggtaaaaag caaactgcaa ctggggggcg tattccatat ggcgaccgac
     8401 tgggaacctt atgcggaaca tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac
     8461 ctgtcagaga gcaatgatta cgtaccgcgt ccggcatcac gtccggtgac gaaatttgaa
     8521 caacgtggtc atcgtcttgg tcacggagta tgggacttaa tgttcgagag ggtgaaataa
     8581 tggcaaagaa ccgtagccgt cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat
     8641 taggattttc ggtggcatgg cgattcccgg aaggtacatc ggaagaacag attgataaaa
     8701 ccgttgatga ctttattaac gaggttatcg aaccgaacaa actggccttt gacggcagcg
     8761 gttatctggc ctgggaaggt ctgatctgca tgcaggaaat cggcaaatgc accgaagaac
     8821 atcaggcgat tgtgcgtaag tggctggaag agcgcaaact gggcgaggta cgcaccagcg
     8881 aacttttcga cgtttggtgg gactaagaaa gcatacgggc gatgacaaat gcaaaactgc
     8941 ctgatgtgct acgcttatca ggcctataaa gatgcacgat cgagtaggct ggataaggcg
     9001 tttacgccgc atccggcatg gaaaacgcgc actttgttat caatctgggg ccagcaaatg
     9061 ctggcctgat ttgttcttga gggaagacta tgatgcgcaa aatgctgctg gcggcagcac
     9121 tttcagtgac ggcaatgacc gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg
     9181 atgtgattgt cagcccgcaa accgtgcagg tgaagggcga aaacggcaat ctggtgatca
     9241 cgccagacgg caacgtgatg tataacggta agcaatattc cctgaatgcc gcccagcgcg
     9301 agcaggcgaa ggattatcag gctgaactcc gtagcaccct gccgtggatt gatgaaggcg
     9361 cgaaaagccg cgtcgaaaaa gcccgtattg cgctggataa aattatcgtt caggagatgg
     9421 gcgaaagcag caaaatgcgc agccgtctga ccaaacttga tgcgcagctg aaagagcaga
     9481 tgaaccgcat tatcgaaacg cgcagcgatg gcctgacgtt tcactataaa gccattgatc
     9541 aggttcgcgc cgaaggccag caattagtga atcaggcaat gggtggaatt ttacaggaca
     9601 gcattaatga aatgggcgcg aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg
     9661 tgctgggaag cctgggcggt ctgcaatcct caatccaaac cgagtggaaa aagcaggaaa
     9721 aagatttcca gcagtttggc aaagatgttt gtagccgcgt tgtgactctg gaagatagcc
     9781 gcaaagccct ggtcgggaat ttaaaataat cctctatttt aagacggcat aatacttttt
     9841 tatgccgttt aattcttcgt tttgttacct gcctctaact ttgtagatct ccaaaatata
     9901 ttcacgttgt aaattgttta acgtcaaatt tcccatacaa agctaaggga taatgcgtag
     9961 cgttcacgta actggaggaa tgaaatggag tttttcaaaa agacggcact tgccgcactg
    10021 gttatgggtt ttagtggtgc agcattggca ttacccaata tcaccatttt agcaactggc
    10081 gggaccattg ccggtggtgg tgactccgca accaaatcta actacacagc gggtaaagtt
    10141 ggcgtagaaa atctggttaa tgcggtgccg caactgaagg acattgcgaa cgttaaaggc
    10201 gagcaggtag tgaatattgg ctcccaggac atgaacgatg atgtctggct gacactggcg
    10261 aaaaaaatta acaccgactg cgataaaacc gacggcttcg tcattaccca cggtaccgac
    10321 acgatggaag aaaccgctta cttcctcgac ctgacggtga aatgcgacaa accggtggtg
    10381 atggtcggcg caatgcgccc gtccacgtcc atgagcgcag acggtccatt caacctgtat
    10441 aacgcggtag tgaccgcagc tgataaagcc tccgctaatc gtggcgtgct ggtggtaatg
    10501 aacgacaccg tactggacgg tcgcgatgtc accaaaacca ataccaccga cgtagcgacc
    10561 ttcaagtctg ttaactacgg tcctctggga tacattcaca acggtaagat tgactaccaa
    10621 cgtaccccgg cacgtaagca caccagcgat acgccgttcg atatctctaa gctgaatgag
    10681 ctgccgaaag tcggcatcgt ttataactac gctaacgcat ccgatcttcc ggctaaagca
    10741 ctggtagatg cgggctatga tggcattgtt agcgctggtg tgggtaacgg caacctgtat
    10801 aaaaccgtat ttgacactct ggcgaccgcc gcgaaaaacg gcactgcagt cgtgcgttct
    10861 tcccgcgtac cgacgggcgc taccactcag gatgccgaag tggatgatgc gaaatatggc
    10921 ttcgtggcct ctggcacgct gaacccgcaa aaagcgcgcg ttctgctgca actggctctg
    10981 acgcaaacca aagatccgca gcagatccag cagatcttca atcagtacta atcacctcgc
    11041 cccggtaacg tgccggggct ttttcacttc agacttacgt ccattgccaa ttttcattac
    11101 cctgatgata atcactggaa taaattattc cgcgcgaggg ttttcgggtg aaaaagcaat
    11161 ggattgttgg cacggcgctg cttatgttga tgactggtaa tgtctgggca gatggcgaac
    11221 cgccaactga aaatatctta aaagatcaat tcaaaaagca gtatcacggc attctcaagc
    11281 ttgatgtcat caccttaaaa aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg
    11341 aaggcgatgt ctcttccagt gacgatctct atacctgggt cggtcagttg gcagattacg
    11401 aactgctcga acagacctgg acgaaagata aaccgataaa attctcggcg atgttaacca
    11461 gtaaaggaac gcccgcgtct ggctggtcgg tgaactttta ctcttttcag gcggcagcca
    11521 gcgatcgtgg acgggtggtc gacgatatca aaacgaataa taaatatctg atcgtgaata
    11581 gcgaagattt caattatcgc tttagtcagc ttgaatcagc gttgaataac cagaagaact
    11641 cgattcctgc gctggaaaag gaagtgaaag cgcttgataa gcaaatggtg gcggcgcaga
    11701 aagcagcgga tgcatattgg ggaaaagatg caaacggtaa gcaaatgacc cgcgaagacg
    11761 cctttaaaaa aattcaccaa cagcgtgatg agtttaataa acagaacgat agcgaggcgt
    11821 tcgcggttaa atatgacaaa gaggtttatc aaccggcgat tgcggcatgt cataaacaga
    11881 gtgcagattg ttatgaagtg ccgattcagc agaagcgaga tttcgatatc aacgaacaac
    11941 ggcgacagac ttttctgcaa tcacaaaaac ttagccgtaa attgcaggat gactggataa
    12001 cgctcgaaaa agggcaatat ccgttaacga tgaaagtttc agaaataaac agtaaaaaag
    12061 tcgcgatttt gatgaaaatt gatgatatca accagactaa tgaacgctgg aaaaaagata
    12121 ccgaacaatt gcggcgcaat ggagtgatta agtaactcat gttaagtacc tttgccggat
    12181 aatataaaaa gttatccggc aaattatttt ttacattagc cctagtagct gtggaatgaa
    12241 caaggatatt tgcgggatgt aagtgatcat catcaacgcg attaacaatg cggcataaaa
    12301 tggcagcaat ggtttgatca aatgttgaat cttcacacct gaaatagaac aaccaacaaa
    12361 tagcgcacta cccaccggcg gtgttaacag cccaatacaa aggttagcca ccatcataat
    12421 accgaaatgt accggatcca ttcccagctc ttgtgcaatc ggcaagaaga ttggtgtgaa
    12481 gattagtaca gccggtgtca tgtccataaa gatgccgaca attaataaga cgatattaat
    12541 cagcagcaat atgaccagtg ggttatcaga aatccccatc aacgcgtcgc tgatcatata
    12601 aggaatatca gcattggtca ttgcccagga catgcccacg gaaaaaccga tcaacagtaa
    12661 tacaatcgaa gtggtaacaa ccgattccag aatcagtttg ggtaaatcgc gccattttac
    12721 ttcccggtaa atcagcaccg agagaataaa tgtgtagaca acggcaattg ctgacgcttc
    12781 agttgcggta aaaatgccgc ccagaatgcc gcccattaca atgaacacca gaagcaaact
    12841 tggtaaggca tccagaaacg ctttacatgc ctgagcaaag gttgctcttt ctgaaagtgg
    12901 gtacccacgg cgtttagcaa taatgccgca gactatcatt accgcgagac ccattaagat
    12961 ccctggcaaa tatcccgcca taaatagcga tgctacagaa acgccgccag ctgtcaggga
    13021 aaacacaatt aacacgtttg agggcggtat cagtaatcca gtaatacaag atgagacgtt
    13081 tacagcggta gaaaatgccg gatcataacc ttctttggtc tgaatgggat tcagtgtgcc
    13141 accaacggca gcagctgccg caacggctga accagaaatg gaaccaaaca tcatattcgc
    13201 caggacgttc acatgaccaa gcgaaccagg aacgcggcca accattacct gggcgaggtt
    13261 aatcaaacgt atcgcaatac cgccgctgtt catcagcgtt ccggcaaaga tgaaaaaggg
    13321 gattgccaat agggcaaagt tatccagtcc attagccagt cgctgcgaaa cagtgattgc
    13381 cgcgatatcc cagggaaaca tcaacagcat tgaagctaca gtggcaatac cgattgagaa
    13441 cgaaatgggc acaccaataa agaccagcac aaaaaaacag ccaaacagtg taagcgcgat
    13501 ataactatcc attaactgac ctcctgcgca ggttgtttca gttgttgaat gctttgatta
    13561 ataaaacaca aggcataaaa catcatcacc aatccactta atggcagaat gatatatacg
    13621 tatcccatcg gtatttgcat cgcggcagat acctgtgatg ttgccagtgt tttatcaatt
    13681 aatttcaggc caccagtcac aatgaccgaa ccggcaaaac ctaaaataag aacgttaatg
    13741 actatactaa gaagtaattg cttgcgctta ttcagggcga gagcaaataa atcaatagag
    13801 agatgtctct gcgcgccgac ggtatatgca gcacctaata atcctaccca gatcatcaaa
    13861 aagcgtgcca gctcatcggt taatgtactt ggctggttaa gtacgtagcg gctaaatact
    13921 tgccagacta cgcatacaac cagagcaagc attacggcga cagaaaacgc ggcaatgacg
    13981 cggtctaccg caagtttcag gcggttaaat atcattgtaa gcatcctgga aaatcagggc
    14041 cagagactgg cccgaaatat tattgagcag cgttgtcgaa tttctctaac aaagcagctt
    14101 gttttggatc ttttttgaag tcgtcaaaca gaggctgaac tgcggcacgg aatggggctt
    14161 tatcaacctt aacgatttca ccacccatgg ctttcgcctg agcacgtgtg tcagcgtcaa
    14221 ttttttccca caatttttgt tgataagctt cggatttctt cgccgctgtt tcgaggattt
    14281 gttgctgttc tggggttaat ttattccatg ttttggtgga gataaccaaa aagtcaggaa
    14341 ttgaggcatg ttcatcttca gaaaagactt tggcaatttc gatatgtcga gtctgcaccc
    14401 aggaaggcac gttattttct gcgccatcta ctacaccttg ctgcatcgca gtataaactt
    14461 cgccaaatga aattggagtg ggtgagccac ccatcagttc aatcattttg atggtggtcg
    14521 ggcttggctg aacacgaatt ttcagccctt taagatcttc tggcttagta atcggttttt
    14581 tcgcatagaa actgcgtgta cccgctacat aagctgataa agcaaaaaag cctttatctt
    14641 ttgttgaatc cataatttct ttaccaactt caccaaaaac cactttgttg aagtgcgcct
    14701 gatctttaaa taagaacggc aaattataga tagcataaat attatcaaaa gactccagat
    14761 cactggcgga acctttggtc atatccagtg caccattttg caacaactac atagtttctc
    14821 gcgcattacc catctggctg ctggggtaaa tacggatcac catattacct tttgacagct
    14881 gttttacttc tttcgccagc tcttcaaaac tctgatgtac aacatgactt ctttccaggt
    14941 tgtgtgctaa ctttaacgtc actttttctg cggcgtttac gccagtgcta accagagcca
    15001 gcaaggatgc gcagactaat gagcgggtaa atgacctcat tttcataaaa aaactccctg
    15061 ttgaagaatg gaaattggtt gatatctaat tgtgcttatc agtgctttgt gcgacaaatt
    15121 ggttaacctg tctgacaact tgtctatgtt tatcacaact tgaaataaag tcagaaatta
    15181 aatattaatt ttgtgcgc
//
LOCUS       DAZGHF010000116        15069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGHF010000116 DAZGHF010000000
VERSION     DAZGHF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.117"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(148..1200)
                     /gene="fbaB"
                     /locus_tag="ACHK3A_003748"
     CDS             complement(148..1200)
                     /gene="fbaB"
                     /locus_tag="ACHK3A_003748"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO8741777.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1457..2734
                     /locus_tag="ACHK3A_003749"
     CDS             1457..2734
                     /locus_tag="ACHK3A_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO8741778.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2731..3735
                     /locus_tag="ACHK3A_003750"
     CDS             2731..3735
                     /locus_tag="ACHK3A_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO8741779.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAYFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            3732..4697
                     /locus_tag="ACHK3A_003751"
     CDS             3732..4697
                     /locus_tag="ACHK3A_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO8741780.1"
                     /translation="MSGARLHTLLPELATRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAATLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4671..5417)
                     /locus_tag="ACHK3A_003752"
     CDS             complement(4671..5417)
                     /locus_tag="ACHK3A_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8741781.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5469..6287)
                     /locus_tag="ACHK3A_003753"
     CDS             complement(5469..6287)
                     /locus_tag="ACHK3A_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO8741782.1"
                     /translation="MQLRITSRKKFTALLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(6352..7152)
                     /gene="thiD"
                     /locus_tag="ACHK3A_003754"
     CDS             complement(6352..7152)
                     /gene="thiD"
                     /locus_tag="ACHK3A_003754"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO8741783.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWTDTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7149..7937)
                     /gene="thiM"
                     /locus_tag="ACHK3A_003755"
     CDS             complement(7149..7937)
                     /gene="thiM"
                     /locus_tag="ACHK3A_003755"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO8741784.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVASSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(7972..8068)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8160..8432)
                     /gene="rcnR"
                     /locus_tag="ACHK3A_003756"
     CDS             complement(8160..8432)
                     /gene="rcnR"
                     /locus_tag="ACHK3A_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO8741785.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8554..9378
                     /gene="rcnA"
                     /locus_tag="ACHK3A_003757"
     CDS             8554..9378
                     /gene="rcnA"
                     /locus_tag="ACHK3A_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO8741786.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGKRNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            9597..9935
                     /gene="rcnB"
                     /locus_tag="ACHK3A_003758"
     CDS             9597..9935
                     /gene="rcnB"
                     /locus_tag="ACHK3A_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO8741787.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPDMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10017..11051)
                     /locus_tag="ACHK3A_003759"
     CDS             complement(10017..11051)
                     /locus_tag="ACHK3A_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO8741788.1"
                     /translation="MEIRIMLFIVMMMVMPVSYATCYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLALSYNPITTTCKPDDLLINLESIPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAEDNGVGFML
                     ESNSSPVTFLNITDNSKGYTNLKEIAAKSKITDTTVSIPVTASYYVYDKNKVKSGALQ
                     ATALINVKYD"
     gene            complement(11067..13547)
                     /locus_tag="ACHK3A_003760"
     CDS             complement(11067..13547)
                     /locus_tag="ACHK3A_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO8741789.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSIQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPFRGAVVL
                     VNFDTDQRKPWFIKALRTDGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCAITFGKEIDESRNYICQ"
     gene            complement(13563..14237)
                     /locus_tag="ACHK3A_003761"
     CDS             complement(13563..14237)
                     /locus_tag="ACHK3A_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8741790.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSENWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(14318..14860)
                     /locus_tag="ACHK3A_003762"
     CDS             complement(14318..14860)
                     /locus_tag="ACHK3A_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8741791.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDISAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         3742 a         3778 c         3486 g         4063 t
ORIGIN      
        1 acgacaatat gaaaatcaat ccaggaatga aaggcaaaaa aaacggcctc ccgggggaag
       61 ccgtaacaaa gtacgcgtgg ttttatgccg gatgtggcgt taacgccttt tccggcctac
      121 ggggtagtgc tgttcgtatg aagcgcatca ggcgatagta attttgctat cgagataaac
      181 gtcctgcacg gcgttaatca gtttcacgcc gtcagccatc gatttcttga acgccttacg
      241 tccaagaatc agccccattc cgcctgcgcg tttgttgata accgcagtac gcactgcatc
      301 gctgaggtca gtttcaccgc ctgcagcacc gccggagttt atcaacccgg cccggcccat
      361 atagcagtta gctaactgat aacgcaccag gtcaatcggg ttttcactgg tcagtttgct
      421 gtagactcga tcgtcggtat aaccgtaatt aatcgcttta tagccgccgt tattttccgc
      481 cattttttgt ttgacgatat ctgcacctat ggttgccgcc agatggttcg cctgaccagt
      541 caggtcggcg gaaacatggt aatcaacgcc atctttctta aaggcggagt tacgcaaata
      601 ggcccacagc actgtcacca tgcccagctc gtgcgcacgt tcaaaagccg cagaaatttc
      661 ttcaatctgg cgacgtgact cttccgagcc aaaatagata gtcgcaccaa ccgccaccgc
      721 gcccatattg aacgcctgct ccacgctggc atacagcgtt tgatcgtagg tattcgggta
      781 acttagcgtc tcgttgtgat taagtttgac gaggaatgga atgcgatgcg cgtaacgccg
      841 cgataccgat gccagcacgc cgtaagttga cgccacacag ttacagcccg cttcgatcgc
      901 cagttcaaca atgtttttcg ggtcaaagta gagcgggtta gcggcaaatg aagctccggc
      961 agagtgctca acgccctggt caaccggcag aatagaaaga tatcctgtgc cagccagacg
     1021 cccggtgttg tacaacgtct gcatattacg taacaccgct ggcgggcgat tattgtcaat
     1081 catcacgcgg tctacgtagt catgtccggg gagataaagc tggtcagaag gaatagtcat
     1141 acaacggtgc tgtaaaaggt tgtcggcgtc tttgccaagc aactgcgcaa tatctgtcat
     1201 tactatgctc ccgtaaattc cgattggata tcggctatgg attttcctgg cccgcctttt
     1261 gcgggcaatc ataatcctgg tcgttacagg cgagtttttc catcttttgg atgttttttc
     1321 agcgttttct gttggcacga ttcatcagaa aaaatgttat cgcggtcaag ttttcaccgc
     1381 aaaggtattt aaaaggtatt attaaatggt attgtcatcg cgtaccttac attacctgtc
     1441 atgaaggaat taaaagatga aaacaacagc aaagctgtcg ttcatgatgt ttgttgaatg
     1501 gtttatctgg ggcgcgtggt ttgtgccatt gtggttgtgg ttaagtaaaa gcggttttag
     1561 tgccggagaa attggctggt cgtatgcctg taccgccatt gcggcgatcc tgtcgccaat
     1621 tctggttggc tccatcactg atcgcttttt ctcggcgcag aaagtgctgg cggtattgat
     1681 gttcgctggt gcggcgctga tgtatttcgc tgcgcaacag accacttttg ccgggttctt
     1741 cccgttactg ctggcctact cgctaaccta tatgccgacc attgcgctga ctaacagtat
     1801 cgcttttgcc aacgtgccgg atgtggagcg tgatttccca cgcattcgtg tgatgggcac
     1861 tatcggctgg attgcctccg gtctggcatg tggatttttg ccgcaaatgc tgggttatgc
     1921 cgatatctct ccgactaaca tcccgctgct gattaccgcc ggaagttctg ctctgctcgg
     1981 tgtgtttgcg tttttcctgc ccgacacgcc gccaaaaagc accggcaaaa tggacattaa
     2041 agtcatgctc ggcctggatg cgctgatcct gctgcgcgat aaaaacttcc tcgtcttttt
     2101 cttctgttca ttcctgtttg cgatgccact ggcgttctat tacatctttg ccaacggtta
     2161 tctgacagaa gtcggcatga aaaacgccac cggctggatg acgctcggcc agttctctga
     2221 aatcttcttt atgctggcat tgccgttttt cactaaacgc tttggtatca aaaaggtatt
     2281 gttgcttggt ctggtcaccg ctgcgatccg ctatggcttc tttatttacg gtagtgcgga
     2341 tgaatatttc acctacgcgt tactgttcct cggcattttg ctgcacggcg tcagttacga
     2401 tttttactac gttaccgctt acatctatgt cgataaaaaa gcccccgtgc atatgcgtac
     2461 cgccgcgcag gggctgatca cgctctgctg ccagggcttc ggcagtttgc tcggctatcg
     2521 tcttggcggt gtgatgatgg aaaagatgtt cgcttatcag gaaccggtaa acggactgac
     2581 tttcaactgg tccgggatgt ggactttcgg cgcggtgatg attgccatta tcgccgtgct
     2641 gttcatgatt tttttccgcg aatccgacaa cgaaattacg gctatcaagg tcgatgatcg
     2701 cgatattgcg ttgacacaag gggaagttaa atgaaaacag aacgtattct cggtgctctt
     2761 tatgggcagg cgttagggga tgcgatgggg atgccctccg agctttggcc gcgcagtcgc
     2821 gtcaaagcgt actttggctg gattgaccgt tttctgcctg ggccaaagga gaataacgcg
     2881 gcctgttatt ttaaccgcgc cgaattcacc gacgatacct cgatggcgct gtgtctggcg
     2941 gatgcgttac tggaacgtga aggcaagatc gatccggatc tgattgggcg taatattctc
     3001 gactgggcgc tgcgtttcga cgcctttaac aaaaacgtac taggtccgac ctcgaagatt
     3061 gctctcaacg ccattcgcga cggtaaaccc gttgctgaac tggaaaacaa cggcgtgacc
     3121 aacggcgcag cgatgcgcgt ctcgccatta ggttgtttgc ttccggcgcg tgatgttgat
     3181 tcctttattg atgatgtggc gctggcctcc agcccgacac ataaatccga tctggcggtt
     3241 gcgggggcgg tagtcatcgc atgggcgatt tctcgtgcca ttgacggaga aagctggtca
     3301 gcgattgttg attcactgcc ttcaattgcg cgacatgctc aacaaaaacg catcactacc
     3361 ttcagcgcct cactggcagc acgtctggag attgcgctga aaattgtgcg caatgccgac
     3421 ggcaccgaat ccgccagcga acagctttac caggtcgttg gcgcaggtac cagcactatt
     3481 gagtccgttc cgtgcgccat tgcgctggtt gaactggcac aaaccgaccc gaaccgttgc
     3541 gccgtcctgt gcgctaacct tggcggcgac acagacacca tcggtgctat ggcgacggca
     3601 atttgtggcg cgttgcatgg cgttaacgct atcgatcctg cgttaaaggc ggaactggat
     3661 gcagtaaatc agcttgattt caaccgctat gccacggcgc tggcgaagta tcgccaacaa
     3721 cgggaggcga tatgagcggc gctcgattac acacgctgct gcctgaatta gccacgcgtc
     3781 agccagtgat ggtcgtcggc gcggcggtca ttgatgtgat cgccgacgct tatgccctcc
     3841 cctggcgtgg gtgcgatatc gaactgaaac agcagagcgt taacgttggc ggctgcgcac
     3901 tgaatattgc cgtggcgtta aagcgcctcg gcatcgaagc gggtaatgcc ttgccgctcg
     3961 gtcagggcgt gtgggcggag attattcgca accggatggc aaaagagggc ttaatcagcc
     4021 tgatcgataa cgccgaaggt gataacggct ggtgtctggc gctggttgag ccggatggcg
     4081 aacgcacttt tatgtcattc agcggtgtgg aaaatcagtg gaatcgccag tggctggcgc
     4141 gattaaccgt tgcgcctggc agcctgctct atttttccgg ttatcaactg gcctcgccct
     4201 gcggcgaatt gttagtggaa tggctggaag agctgcaaga cgtgacgcca tttatcgatt
     4261 ttggcccacg tattggcgat atcccggatg cattactggc gcggatcatg gcctgtcgac
     4321 ctttagtgtc actcaatcgt caggaggctg agattgccgc cgaacgtttt gctttatccg
     4381 cagagataac aacacttggc aagcaatggc aggagaaatt tgctgcgacg ttgatcgttc
     4441 gtctcgataa agagggcgca tggtatttca gcaacgacgc ttctggctgc attccggcat
     4501 ttccaacgca agttgtagac accattgggg cgggcgacag tcatgccggc ggcgtacttg
     4561 ccgggctggc ctctggtctg ccactggcgg atgccgtatt actgggcaat gcagtggcgt
     4621 cgtgggttgt cgggcatcgg ggcggtgatt gtgcgccaac gcgcgaggaa ctactcctcg
     4681 cacacaaaaa cgtatagatc gctgcgacag tggctgatgc tgtactcaat aggccgttgc
     4741 tgttggtcaa gcgccacttg cttgatcacc agcaccggta ttttgctatc taactgaata
     4801 tgtgactgaa actcggcatc cggcatccgg gcgctaacgc gggaacgcgt acgttgtggg
     4861 taaatgtgct ggctgcggaa gtagtcataa agagaaatcc cgatggcatc aacatcatga
     4921 attaaatgtg ccggaaccca cgattcctca atcgataccg cctcttcatc gacatagcga
     4981 atacgcttaa gcaaaaagac atcacttcct gcttcgaccg ctagctgctg cgcgacttct
     5041 tcgggacatt tcacgacacg cttgttaacc catagcgtat cgggcttttt cccgcgcaat
     5101 accacctgct gagaaaaacc tcgcgcttct ttcaacgagt attcgaagat gttgttgatc
     5161 tgcgtgccgt aaccacgcga acgcgtcacc acgccctctt cttccagcgc ctgcatcgcc
     5221 ttgcgcaccg taatgcgcga aacgccggtt aactgactta aatcacgctc accgggcaaa
     5281 atattgccat gctccagcac cccgctgcgc acggcatttt ttaccgtttc ggcaaactta
     5341 atataaagcg gcgtgttatc cgccgctaaa atacgttcgt tcagttgggc aatcagctgg
     5401 gtatgcgctt gttccatttg tctttttcct gccagggagt ctggtgggag tatatcactg
     5461 cttcttattt aaggcgtttc tttaatccca tcaacgaatc cctgcaactc ctccaccgtg
     5521 ccattaaaca cattaaaatc caccggacca ttaatgccat ctacctgtcc acggtcggaa
     5581 tgctgccaga agcgccaggc cattccgtca ttgtccggac gacgttgata gtagtgagcc
     5641 acccaccacg gatattcatt aaaatagccc gccagattgg tgtgataaaa aacggctcct
     5701 gagtaaataa ttggcttttt tcccgtacgt ttttcgacca tttttagcca ctgacttacc
     5761 cgcttgcgta attcttttgc cgataatttt cctcgctctt ctacgtccag taccgcaggg
     5821 aaatcgcctt gtgagaaatc caccgtttgc agaaataatc gcgcctgaac tgaagcggat
     5881 actgacgggg agaaataatg atacgccccg cgcagcaggc cattttcgcg gcttagttgc
     5941 cagttacgcg aaaaataggg gtccaccagc ttttcgcctt ccgtcgcctt aataaaagca
     6001 aactgtaagc ggataccgtt gtcgcgcatt tttgccaccc gctgccagtc gatccgctcc
     6061 tgccagcgcg aaacatctat tccgtgaatg gtgtaactgg caggaatgcg aatagcgaaa
     6121 gatttaaccg ggcgataacc gtagaagtgg atgtagtctt taatctgaat tgctgtcgaa
     6181 tagaaaaaat tcaccgtctg acgcggataa attgcaacga tggaaattag cccaagagcg
     6241 cataataaag cggtgaattt ttttcgactg gtgattctta gctgcattgg tttcatccct
     6301 gaatgtcagc gccaaaggct gacaataacc aaagcagcta tagtacggtg ctcaccacca
     6361 ggcgtggaag tggtgaaccg gaccaatacc gtgaccaact tccagcgtgt cggcctgggc
     6421 taatgccgat gaaagccagc tttttgcctc ctgtacggtg tcagtccagt ttgtatggcg
     6481 cgggcgcagt gcagccaacg ccgcagagag cgtacaacca gtgccgtgag tgtttttggt
     6541 cataatgcgc tgtgcggtaa accgttgttc accctcgcgg gtaaacagcc agtccgggct
     6601 ttgctcatca tccagatgac cacctttcat tagcactgcg ccacagccca tcgccaacag
     6661 cgatcgccct tgttccagca tttcctgttc ggtgcgcgcg tgtggcgcgt cgagcaaggc
     6721 ggctgcttcg ggcaagtttg gcgttattaa tgaaacctgt ggcaataatc gactgcgcag
     6781 cgtagcaacc gccgaaggtg aaagcagcgg gtcgccgctt tttgccagca taacggtgtc
     6841 gagtaccacg ttttggatct gataacgttg caaccgttct gccaccgctt caacaatatc
     6901 ggtttccgcc aacataccga ttttagtggt gtcgattcgc acatcgctga acaccgaatc
     6961 gagctgggcg gcgacaaaat caggctcaat gcgatacacc gactgtacac cacgggtatt
     7021 ttgcgccacc agtgcagtaa taactgagca accataagcg ccaagtgccg agaaggtttt
     7081 aagatcggcc tgaatccccg caccaccact cggatcagta ccggcaatcg tcagagcgtt
     7141 aattcgtttc acgcttgcac ctcctgcgtc aattgccaga gcgcatcaag gaaatgtgga
     7201 acaaaactgc ctggcccctc gctactggcg actgcgcgtt ctccggcttg tttcatccag
     7261 tgacaggcag atgcgatatt ttccagcgta tcgcctggta gcgcacaaca ggcagcgaca
     7321 accgccgata atgcacagcc agttcctacc actttggtca ttaacggatc gccaccgtga
     7381 atgccaatga tacgatgtcc atcggtaaca taatccacct cgccagtgac cacgacgatt
     7441 gcgccagttt cccgtgccag tgtttgtgca gcgggtatcg cgttagctgc ggcatcagtg
     7501 gtatccactc cccgtccacc attagcaacg ccagctaatg ccatgatttc cgaagcatta
     7561 ccacgtatgg cggttggctt atgggacaga agttccacac aaaaacggcg gcgataatcg
     7621 agcgcaccaa ccgcaaccgg atcaagcgtc cagggcgttt gtgatcgtgt tgcttgctca
     7681 acggcagcac tcatcgcttg cgctcgtggc tgcgtcagcg tgccaacgtt aatcaacaag
     7741 gcactggcga tagccgcaaa ctgactggcc tcttcggttt cgataaccat cgctggcgat
     7801 gcaccgatcg ccagcaaggt attggcagta aaagtttgca ccacatcgtt ggtcatgcag
     7861 tgcacaagag gggaatgttg gtgaaaaagg tgtaacgtgt gcgcagattg cgcggaactc
     7921 agcaggtcga cttgcatagt ttgctcctgc cataacgtga agaagcaatg acctggtggt
     7981 ccgtgacttc cctacgctgg cattatccag atcaggtgat acgggtattt ctcagccttc
     8041 acaaagaagg gcaccccgag tcgtttggtt gcgataataa atcgcagagg aggatggtaa
     8101 tgtccagcgc acgcgttgta aacggggaaa aacggcggta ttacaatcgc gttaataaat
     8161 tatttgatat aagaatccag caccttcaga acgacatcca gatcttcttc acgttttagc
     8221 tcatccccct ggtgaacgat gtgttccgtc agatggcctt taatcacttc ccgcatcaga
     8281 ccgtttaccg cgccacggat agcagcaatc tgttgtaaaa ctgcagcgca ttcatgcggt
     8341 tcgtcgagca ttttcttgag cgccacgacc tggccctgaa tcttactggc acgcgctttc
     8401 agtttctgtt tatcacggat tgtatgagac atggcaacac ctggttaaca agaatatgaa
     8461 aaatcatagc actattaatc tactgggggg tagtataagg tactgggggg ggagtagaat
     8521 cagattgccg aattaatact aagaattatt atcatgaccg aatttacaac acttcttcag
     8581 caaggaaacg cctggttctt catccccagc gccatcttac ttggtgcgct tcatggcctg
     8641 gaaccggggc actcaaaaac gatgatggcg gcgtttatca tcgccatcaa aggcaccatt
     8701 aaacaggcgg tgatgctcgg actggcagca actatttcgc ataccgcagt agtctggtta
     8761 attgcctttg gcgggatggt gatcagcaaa cgctttactg ctcaatcagc agaaccgtgg
     8821 ctccagttga tttccgcagt gatcattatt agcaccgcgt tctggatgtt ctggcgtacc
     8881 tggcgcggca aacgcaactg gctggagaat atgcacgagc atgatcatga gcatcatcac
     8941 cacgatcacg aagatcacca cgaccatgga catcatcacc atcacgaaca tggcgagtat
     9001 caggatgccc atgcacgagc ccatgccaat gacattaaac gacgctttga tggtagagag
     9061 gtcaccaact ggcaaatttt gttatttggc ttaaccggtg gccttatccc ctgcccggca
     9121 gcaattaccg tgctgttgat ttgcattcag ttgaaagccc tgacactggg cgcaacactg
     9181 gtcgtcagtt tcagcatcgg cctggcgtta acgcttgtca ccgtaggcgt tggcgcagca
     9241 atcagcgttc agcaggtcgc aaaacgctgg agcggattta acactctcgc taaacgcgct
     9301 ccctattttt ccagtctgtt gattggctta gttggtgtgt atatgggcgt acatggcttc
     9361 atgggcataa tgcgataact caatccttta ggcttgcatg ttatcttcag cccaggatga
     9421 ttcttaaatc agctatttcc gctgactctc tgctcgctaa ggatgctttt agggcatcct
     9481 ttcatttaca ctttttacga aatcatggga tcactaacaa aatatcgctt gtcagttata
     9541 ttgtatggca ggaaagatat gcgactgata ttacagatcc ccaaagtgga gagtttatga
     9601 ccattaaaaa taagatgttg ctgggtgcgc ttttgctggt taccagtgcc gcctgggccg
     9661 caccagccac cgcgggttcg accaatacct cgggaatttc taagtatgag ttaagcagtt
     9721 tcattgctga ctttaagcat ttcaaaccag gcgataccgt cccagacatg taccgcaccg
     9781 atgagtacaa cattaagcag tggcagttac gtaacctgcc cgcgcctgat gccgggacgc
     9841 actggaccta tatgggtggc gcgtacgtgt tgatcagcga caccgacggt aaaatcatta
     9901 aagcctacga cggtgagatt ttttatcatc gctaaaaaaa gccccctcat catgaggggg
     9961 aaatgcagac accttgttat tttttattat tcgccacttg ctcgtcttgc ttattattag
    10021 tcgtatttca cattgattaa tgcggttgcc tgtagtgcac cagattttac tttattttta
    10081 tcataaacat agtaactggc agttactgga attgagaccg ttgtatctgt aatttttgac
    10141 ttcgccgcaa tttcttttaa atttgtgtac cctttgctgt tatcagtgat atttaaaaat
    10201 gtgactggcg aactattgct ttcaagcata aatccgactc cattatcttc tgcacctttc
    10261 aggatcgtgt tactgccggc taacaaatca gaacttgata aatacacctg catttttcgt
    10321 gatgtttgat tttgttgacc taataaattt ttacaacgca gaataatatc ccctttttta
    10381 ctatttattg ttgctttgtt acctgtggct ggtaattgtg aaacgggaat actctctaaa
    10441 ttaattaata aatcgtccgg tttgcaggtt gtggttatcg ggttgtatga caatgccaaa
    10501 ttaaaggaat agacacctgc attttgtgcc aatgcatttc tgtagctatt tacacatgca
    10561 tcccatgact tgccacctaa taaacacagt agaaattgta ctgcggcact cccgcgtgtc
    10621 gatgatgaag cactggataa tgttgcaaca ccattaatca tcactgaacc gccgcccccc
    10681 gcagaaacat tcacttttgc attactggcc gcaggtgtca ccgttaccga ataaccactg
    10741 gtgaaactgg tctgattatt tgacaacgta atgttgtttt tattggtcca ttcaaaatgt
    10801 aaatttaatt taatggtatc gttataaagt ccaacaataa tatccgacgg gctcataggt
    10861 gtgatagtat ttgtactcac gcatgacgaa tcattaaaat tatgctcata tgtcgccatt
    10921 tgtgtttgag taagtgcaaa atccccctga acaaccaaat tgtgctgaac agataactca
    10981 ctataacatg tcgcatagct cacaggcata accatcatca ttactataaa tagcattatg
    11041 cgaatttcca tagaaccacc tgactattac tggcaaatat aatttctact ttcatcaatc
    11101 tctttaccga aggtgattgc gcatgaaagc ccttgttgct tatcaattgc cacattaacc
    11161 gatggcggta cttcattggt gcgaataaat aactggcttc cctggccgac aacaccaata
    11221 ttatgaccat ggatatcatt gacttcataa ccaaacgtta atggttgccc atccgttctt
    11281 aacgctttta taaaccaggg cttgcgctga tcggtatcaa aattaaccag tacaaccgcg
    11341 ccgcgaaaag gggcggcaat tttccggttg ccacgtaatt ctgcttcgct atcgctttgc
    11401 gacacatcca gcatcaggtg attttcccga taaggtgtca ttccgtcgta taccaccact
    11461 ccattacggt ttgttgtgcg atatttttgc ccattgacat aagcatcttt aattcctggc
    11521 gcattcatta cagcaaacgt ttcggaaaga cggttcgcca gattaacgcc acccgaccag
    11581 gcgacaatgc cccctgaaac actggctcca gcctgtcgat aagtactcga ctgactataa
    11641 ctgccattca ccgttgccac cggcgcgttc caggttaaat tcgctccagc tgtcgtttca
    11701 tttccctgat gctgataact caggttgacc ccatagttaa actgatcccg gcttccaacg
    11761 gttcctgata atcccgtatt atttgaggca accccctgat catcaaacgt cgttgagtta
    11821 gacatatata tttgccgacg aggcgtcgta acgtcatcac cccaatcaaa gggaatcgat
    11881 ataaaaatat taaaacgttt ctcttcatga tgattctcgt cataagcatg gcttgccgcg
    11941 agggtatagc ttatccttcg caggttgttg gaataactca actgataatc cttgctgctg
    12001 ccgctacgcc cccagtaatc tcgccataac gtacttaagg acacagaacc ccagccttct
    12061 ggcaatgact ggctcatatt ggcagaaaag ctatttttac ggccaaaatc gttctggtaa
    12121 taatcggcaa tgtcatagat atcgttttca tcacggcgat aattatcttt attgtttgcc
    12181 catacgtgat cattaaatgt ccgataatca cgcgacgaat aacgccaggc cgccagacca
    12241 aaacgtgtcg acgtttggct cacaaatttg ttgtaggcaa tttgataact ttgcccgtca
    12301 aacacatcgc cgttgtcttg tatactatgc gacttcgtgg catcgacgga aatggcacca
    12361 atgcgtgtat tccagccagt ccccaaagtg aacgcgtaat aattattcgc gaccatcgat
    12421 ccgccataca gcgtcaataa gttattaaaa ccatactgat aacccgcctg aacaaaatca
    12481 ctttgtttgc tcgccccttc aatatggcta cgacctgccg caaaatcata tttcgacacg
    12541 ccgggttgca gcatattcgg cactgctgca taaggcacca gataggtggt taccgaaccg
    12601 tccgcctctt tcacgctgac atcaagatct gctccaccac cggccaactg caaatctgta
    12661 atcgcaaacg ggccaggagg aacctctttc tgataaacca caaaaccatt ctgttcaata
    12721 gttaccagcg cgttactctg agcaatcccc tgcacccgtg gagtaaaatt ttgtttcgag
    12781 ttaggcaaca tctgcatatc acgaaacaac cgcacaccgc tgaagcgaac agaatcaaaa
    12841 atatcgcttg atgtgtacat atcacccacg cgaagcgtgc cgagaagttg ggcaaatcca
    12901 cgttccagat acagggtatt gcttttccac acccctggat tgctatttgt tttactgaaa
    12961 ctggcatcag aatgcagttg ccaccccagt aagtttaacc cgctgttaaa acgtacatat
    13021 gtactcttgc tattacccga cgctttatag tcgctgtaat actgactcac ataataagag
    13081 gtataaaacg cattaatacc ccgctcccag ttttccggtg gaacatagcc actttccagt
    13141 tcttccaccc acgcctgcgg gacactgaaa tcgagacgaa aaactccgat atcccaggta
    13201 tagctcccac cctgaacaag ttgctcaaat gttaaacatt gcttaccgct ggcaaagtta
    13261 tcggtattaa tgcctaaccg cttgataatt tcccttgata aacatgtttc ttgcgggttg
    13321 tctttaacaa taatctcata tttcccgcgc cattgcttat tgacataaat atcgatgtca
    13381 tactgccccg gcaagggttg attatcatca agacgaatat ttgcaacctg ctggtctttc
    13441 attccaccta tcatgaaatg ggtatcaaag gtttcttcag ctgcataagc ttcaatgccg
    13501 agcaataacg ctacgattgc tgatgcaagt ggggtcattc tcaacatagg cagctcctgc
    13561 aattaaattt tgtcactaat atagttgcca tggtcatcga taatggtcag atgccagtta
    13621 ttcgcattat tacttttgac attaatactc tgactttcta agggggcaat cataatagtt
    13681 tcataattga ctttgacatt attagctttg acatccgaaa tcgttaccca attttctgag
    13741 tcatttttta tcaccaatcc attgccactg cgatttacca gtaacttttt aaatgtcgct
    13801 ttatttaccg gagcaatacc cgctggccgg taaaacaact taattctgtt ttgcatcgca
    13861 aacttcagtg cattcttacc ttcttgttct ggactatttg gtggaatatc cagaacattc
    13921 agataaaaaa tactctcttt attagtgggt agtttattcg gcataatttt aatttttact
    13981 tgctgcccgg aatttgcccc tatttttgcc actggtggcg ttaacatgaa aggaacctga
    14041 attttttctg gtggtaatga cgtatcgcca tcatcaatcc acgcctgcag cagcgaagaa
    14101 cggtttccct ggttcatcaa ctgcaccatc acttctttat tttctgccgg gtaaataatg
    14161 cgcgtcccgt agataacaat accggcatgt gcaggaagga ccatagaaga aatgagtaaa
    14221 gctaataatc ctttcatctt aatgcctctt aaattaaaag gccgccatgg ggatagcggc
    14281 catgtttatt acctggagaa acttgcaatt ttatttatta atcctgcgcc acacgcataa
    14341 ccagagaaga taacacatca cctgattgca ctgaattatc tgtcagcttc aggtattttg
    14401 ctttaaacgg aatagcatat tgaccttccg ctgttttata actctcaatg ttaaaaggtg
    14461 cagaaatatc caggacatca ctgttatcac ctaaatagag atagtaaccc acggtatcgt
    14521 tgctactggc tatcgcctta tcgttgccgg ttattttgtc gtttgtgatc aacgagagtt
    14581 ttacaccagc aacttctttc gggcagctaa tttttaacat gctgctggtt acaccgactg
    14641 catcatcata ggtagaatat ttagctttac cttcaaagac agtggttggt acagttggca
    14701 tattaatact tactgaatcg ccacctgctt cgaatttaca cggagattct gcaatattcc
    14761 ctttaatggt taatgttccg gtatcaacgt ctgcggcaaa taccccagcg ctcataaaaa
    14821 aagaagaaaa aacagcggca gcaataattg aacgtttcat aatatgctcc ttaataaaat
    14881 ctatttttgg ttgttaagag tctgtatctc ttggaaacaa atttctcaca cccaaaaagt
    14941 tgccgcaatg taaacacatt gttttattga tttaaagcaa attaaccatt gcaatataaa
    15001 cacaacaaat acttaaacgc attaaatatt tatcaaatct attaattaat gtagatttct
    15061 ataaatggt
//
LOCUS       DAZGHF010000117        15040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGHF010000117 DAZGHF010000000
VERSION     DAZGHF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.118"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..143)
                     /locus_tag="ACHK3A_003763"
     CDS             complement(<1..143)
                     /locus_tag="ACHK3A_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741792.1"
                     /translation="MLQSSADKACNVLVKKVRYLNDVRKITKMMNGKLIYSWLVWPCS
                     EFFS"
     gene            221..1315
                     /gene="ddlA"
                     /locus_tag="ACHK3A_003764"
     CDS             221..1315
                     /gene="ddlA"
                     /locus_tag="ACHK3A_003764"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO8741793.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1518..1730)
                     /gene="yaiZ"
                     /locus_tag="ACHK3A_003765"
     CDS             complement(1518..1730)
                     /gene="yaiZ"
                     /locus_tag="ACHK3A_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO8741794.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1990..2298
                     /gene="yaiY"
                     /locus_tag="ACHK3A_003766"
     CDS             1990..2298
                     /gene="yaiY"
                     /locus_tag="ACHK3A_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO8741795.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2357..3451)
                     /gene="yaiW"
                     /locus_tag="ACHK3A_003767"
     CDS             complement(2357..3451)
                     /gene="yaiW"
                     /locus_tag="ACHK3A_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO8741796.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3464..4684)
                     /gene="sbmA"
                     /locus_tag="ACHK3A_003768"
                     /pseudo
     CDS             complement(3464..4684)
                     /gene="sbmA"
                     /locus_tag="ACHK3A_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
     gene            5036..6193
                     /gene="ampH"
                     /locus_tag="ACHK3A_003769"
                     /pseudo
     CDS             5036..6193
                     /gene="ampH"
                     /locus_tag="ACHK3A_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
     gene            complement(6194..6817)
                     /gene="iprA"
                     /locus_tag="ACHK3A_003770"
     CDS             complement(6194..6817)
                     /gene="iprA"
                     /locus_tag="ACHK3A_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO8741797.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGSCAGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6903..9851)
                     /gene="ehaB"
                     /locus_tag="ACHK3A_003771"
     CDS             complement(6903..9851)
                     /gene="ehaB"
                     /locus_tag="ACHK3A_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO8741798.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWADEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPQGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
     gene            10377..11351
                     /gene="hemB"
                     /locus_tag="ACHK3A_003772"
     CDS             10377..11351
                     /gene="hemB"
                     /locus_tag="ACHK3A_003772"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO8741799.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(11391..12242)
                     /gene="tauD"
                     /locus_tag="ACHK3A_003773"
     CDS             complement(11391..12242)
                     /gene="tauD"
                     /locus_tag="ACHK3A_003773"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO8741800.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWCWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(12239..13066)
                     /gene="tauC"
                     /locus_tag="ACHK3A_003774"
     CDS             complement(12239..13066)
                     /gene="tauC"
                     /locus_tag="ACHK3A_003774"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO8741801.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTFSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(13063..13830)
                     /gene="tauB"
                     /locus_tag="ACHK3A_003775"
     CDS             complement(13063..13830)
                     /gene="tauB"
                     /locus_tag="ACHK3A_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO8741802.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVVDNVVFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(13843..14805)
                     /gene="tauA"
                     /locus_tag="ACHK3A_003776"
     CDS             complement(13843..14805)
                     /gene="tauA"
                     /locus_tag="ACHK3A_003776"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8741803.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         3791 a         3915 c         3636 g         3698 t
ORIGIN      
        1 gaaaaaaact cactgcatgg ccatacaagc caggaataaa ttagcttccc gttcatcatt
       61 tttgtgatct tgcgcacatc attgagataa cgcactttct tcaccagtac gttgcacgct
      121 ttgtcagcag aagattgcaa cattctgtca gaaggccgcg aatcccccgt tttgtcgtgg
      181 taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac tgcgggtagg
      241 aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg caaaaaacat
      301 tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg ataaacaagg
      361 gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc ctgcccatat
      421 tgcgttgcgc ccttcggcga ccagccttgc gcaagtgcca ggtaaacatg agcatcagct
      481 tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc cgattgtcca
      541 cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca atttaccgtt
      601 tgtaggttct gatgttctgg cttcagcagc ctgtatggac aaagatgtca ccaaacgtct
      661 gctgcgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg ctaatcgtca
      721 caacatcagt tttgccgaag tggagtctaa actggggtta ccgctgtttg taaaaccggc
      781 taatcagggc tcttctgttg gtgtcagcaa agtaaccagc gaagaacagt acgcaattgc
      841 cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga taaaaggtcg
      901 tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa gccagcacct gtggcgagat
      961 cgtactcacc agcgatttct atgcctacga caccaagtac attgacgaag atggcgcgaa
     1021 agtggtagtt ccggcagcca ttgcgccaga aatcaacgat aagatccggg cgattgccgt
     1081 tcaggcttat cagacgttgg gatgcgcagg catggcgcgt gtagacgtgt ttttaacccc
     1141 agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca acatcagtat
     1201 gtatccgaag ctgtggcaag ccagcggtct gggttacacc gacctgatca cgcgtttgat
     1261 tgagctggcg ctggagcgtc acgctgcgga taacgcattg aaaaccacaa tgtaattttt
     1321 aaatgcctga taacttttat tgggcattta agactgatgg caaacgtaag attgcctgat
     1381 gcgctttgct tatcaggcct accagataat tgcaatatac tgattttgca tgattttgta
     1441 ggcagggtaa ggcgttcgcg ccgcatccgg caaaaacaac gagcactttg tcagccccca
     1501 gaacgcccaa tcctctgtta ttgggcgttt tcagtttctt catcgcgacg acgaataatc
     1561 agccccgcca gccagaaact aatcacccac gtcaccagac cgacggcata ggtctgccaa
     1621 ccttttgctt caaatcccag taaacccacc acgccattaa gaatgaatat aaggccaatg
     1681 gcgaacgcat agtagtgcca gtcacggcgg atttttacag gcaagttcat ggcagctccg
     1741 ttaacgaaaa acaacatcct tgcatattct gcaacatccg tctgtggcgt agacaacatt
     1801 tccgtaattg ttaataaatt taacttaagg ttgtcatttt gtgactcacg acgaagattc
     1861 acgcttcggg aatattccca acatcaattt accatgattc tgatattgac aatcttaatg
     1921 atctgtcaga cttgccataa gccgctggta tcacaccaga agagaagcag atgacttccg
     1981 gaggttttta tggctgattt caccctgtca aaatcgctgt ttagcggaaa atatcgcaat
     2041 gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg tgctgttttg cttttgggct
     2101 ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg tctatgagcg cttaatgcag
     2161 gtccaggaaa caggtcgccc acgggtggaa attggtttag gtgtcggcac cattttcggg
     2221 ctgatcccgt ttttagttgg ctgcctcatt tttgcagtag tggcgctatg gctgcactgg
     2281 cgacatcgcc gccagtaaga tttacgttgc cagatgcggc ctaaaacgcc ttatctggca
     2341 tgacattcct gtttgatcac tgtttcatac aacgcgcccg ccgttcgtct acgcgcttcg
     2401 caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct ttccagttga atgccaggca
     2461 acatttcgcg gggtagtgat ttacccgttt tcgcttcggc aagttgataa actttcttgt
     2521 acagcgccgt ctcctcaaaa gagaagctgt cccctttctc taactggcgg cgaatttcac
     2581 tgtcgctcat ccccagtttt cccgccagtt tgcgcgttgc cagttcggtt ttcccgggct
     2641 ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg ctggccttac
     2701 tgaccgcgtt ctgaaacgcg gcattacggc tggcgtacca gccagcgtta aaatcagcaa
     2761 aacggtatat cggcgcgcta tagctggcgg gataattcag taaatggtaa gtaccaaacc
     2821 acaacccgcc gcgacgactg aagacttcct gacggactgt accgtccatt ttccacggat
     2881 accctttggt atgctgttcg gcaaaggcaa tgctgacctg catcggccca ccggtgcgca
     2941 ccggattaag cgaaccaaac aacgtctgcc ccattggcac catgctgatc agatcgtcaa
     3001 aaatcgcgct caattgcttt tcagtacgca ccgaatctaa ccgttcgcta tagcttttgc
     3061 cgtttggcga tttgattttt agtgcggtat gcaccagaaa agcaggaatg tgcatccgtt
     3121 cagcacgacg gtcaatctct tgccaggcga tcttgcttaa gcccggaaca gccggatctg
     3181 cctgataatt tgactcctgc tgcgccaccg ccagcaccga gcagacgttt tccagcgtcg
     3241 gtgccagccc ctgactttca aacgtggtgg cgagatcttt cgcccaggca tcgcggtctt
     3301 tcacgctcgc aggcattttc tgccgcacga cgctcgccac atctatcgct ttttcgccct
     3361 ttttcaacgg ttgcggagcc tgactactac atcccgctaa aaccagaacc gccaacaatg
     3421 acagcgaaga tagatgattg acgcgcgaca ttacttctcc tttttagctc aaggtatggg
     3481 ttacttcctg aattttgtca ccatccagct catgttcaaa gctgcgcaga cgtttgtaga
     3541 tagacatcaa ctcaaccagt gtggtccatg agttaatcag gtactggaaa gcaccgcgaa
     3601 cctgaccaaa aacgttagta atctgcgtca tcaggccgag tgtaatcgta ccggcaacaa
     3661 ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag agaatgcggg
     3721 cgatgttgaa atacatatag tgaaaataga ggcggaaata gtttttccgt acggcgctaa
     3781 acagctcgcg taccgtaggc ggcgtcgcac gcgtggcatc gtcttcacca taaaccagct
     3841 ctttacggta ggcagcctct actcgctggt ttttaaactc cagccccggc agtttgatcc
     3901 ctaccactgc cagtaatccg gtccccatca gcgaccagac gatagcggca atcaccagac
     3961 catacggaat gtgcccgaca atcggcagct ccggcacatg tgcggagagc gttaccagca
     4021 ccggcaggaa ggcgatcaac gtcatgatag cgttgataaa gctgacgccc atattctcca
     4081 gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg cgcggcccct tcgatatgac
     4141 gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag cggaacacgt
     4201 agtgactgac aaagaagttg ttcagcacac tgatcaccac cgcgatcagc gcaatcccca
     4261 gaaagactcc cacttcgcgg taaaattgtt cgatggtgac tttatgcggc gaacttagcg
     4321 ccgtttgaat cagatcatag aacggcgcat accaggcgtt gacggcaacc ccgacttcca
     4381 ccaaaaacca ggtgacgaag ataatcagcg cagtaccgag tattgaccag tattgccaac
     4441 ggtgcgggct atagataaac cagaacaatg caaaaagtcc tacgcaaaca atgtagtaag
     4501 cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc gctaatcggg atctgcccgg
     4561 aagcgccggt gatacgcgcc acccaatccc ccccaccggc ttaccagaag ataacggcaa
     4621 tcaatgccca aacaaaggcc gagagaaaaa acgttcccgg ctttgggaaa aaagacttaa
     4681 acatatgcac tcctgctaac ttcttatcgt tttaacaact gctgtgtatt aaccgcatga
     4741 ccgctacaaa gcgtcagcca aatgaaaccg cccattgtga cccatagcaa ggcgcttagt
     4801 tcgccagcct gagcataaga attgtttcga cggatttcac aaaccaaaga ttgtttacat
     4861 aagcggatta acacgcgcag gatcacaaat gcaggcattg gtgagcagga aaaagatcgt
     4921 gtttacgcgc cgttacgttt ttaacactat tattgcacta attcgccctt tgcaatccat
     4981 caatgagtag tataaatacg ctcagttacc ttcattcaat ctatggacac caccgttgaa
     5041 acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg tccatgcggc
     5101 gcagccgatc actgaaccgg agttcgcctc tgatattgtc gatcgttatg ccgatcatat
     5161 tttttacggc agcggtgcca cggggatggc gctggttgtt atcgacggta accagcgcgt
     5221 ctttcgcagt tatggcgaaa cgcgacctgg taataacgtt cgcccgcagc tggattcggt
     5281 catacgtatt gcttccctca ccaagctaat gaccagtgag atgctggtga aattgctcga
     5341 ctaggggacc gtgaagttaa acgatccgct aagtaaatat gctccgccag gcgcacgcgt
     5401 accgacttac aatgggacgc ccattacgct ggtgaatctg gcaacccata ccagcgccct
     5461 gccccgtgaa cagcccggtg gcgcggcaca tcgtccggta tttgtctggc caacgcgcga
     5521 gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc gcgccaggca gtcaggcggc
     5581 gtactctaac ctcgcattcg acctgctggc agatgcactg gcgaatgcgt ccggcaagcc
     5641 ttatacccag ttgtttgaag agcagattac ccgtccattg gggatgaaag acaccaccta
     5701 caccccctca ccggatcagt gccgccgttt aatggttgct gagcgcggtg ccagtccgtg
     5761 caataacacg ctggcggcaa ttggcagcgg cggcgtctat tccacgccag gcgatatgat
     5821 gcgctggatg cagcagtatt tgtcatctga tttttatcag cgcagtaacc aggctgatcg
     5881 catgcagacg ttgatttatc agcgcgtgca gtttacaaaa gtgattggca tggatgtccc
     5941 cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg gccccgaaag agggtcgtcc
     6001 agggattatt cagaagacag gtggtggtgg cggtttcatt acctatatgg cgatgatccc
     6061 gcagaagaac attggcgcat ttgtggtggt cactcgttcg ccattgacgc gcttcaaaaa
     6121 tatgagcgat ggcatcaatg acctggtgac cgagctaagc gggaataaac cgttggttat
     6181 ccccgcatcc tgattaatac tctgaaggca aacggttgat agcgaccagt ttgcctttat
     6241 tcatttcgat atagccgcct ttacgcaaag cagcgagaac ttccgcaacg acagaacgcg
     6301 atatacgtgt acgttgatgg atataattca tcacaccaat acgtgatcgc aattcttcat
     6361 tccagtcaat cattgataat aacgtcgcgc ggatttgttc gtaggaatta tgcccaatga
     6421 gctgaacgtc gcgtaattcc agaattctat tttgccaggc taaccagtaa aaagcgtctc
     6481 gccagagttg attttgttca ataagagtaa tggtttgttt ggctggtaaa tgatatcccg
     6541 cacagcttcc ttctgatatt aatttgtatg gtatatcgtt tttcattaaa ccatcagcca
     6601 gccccataat ataaggagcc tgggtaatac cgataagtac gttttcttct ctacgcagag
     6661 agataacgcc ctctaaaata acgaaagtat cttcgctatt gacatcactg gaaaatataa
     6721 cttgcttttc attattaaac tcgaagcgcg taccgtatct ggacaaacat ttatcgagct
     6781 taccaaattc ctgaagaggt ttaactacag ataacatttg tgcgtccttt gcagtaatgc
     6841 ccgtcaaatc cttgacgggc attatttaga ttaaattacc tatttcttcg gagtgaagaa
     6901 tattaccagg tatatttaac acccacattc gcggaccagt cttgatctac gtcaccacca
     6961 ccgaggtagt tagcatcggt ataggcgctg aagttcttgg tgaagctgaa ctgagtaccc
     7021 agaccaacac gtaccgcaga cccttcagta ccgttatcga tggaatcacc gttcacatcg
     7081 ttatcgttgt tagagtcgtc gtagacgtaa gccagtttga agtacggagt cagagcctgg
     7141 tcttcgctgt aggtgaaggt ataacctgca tctacaccca gttcataacg catgctgtcg
     7201 taagactgac catcaacttt catgtcgttg ctcagctggt aatcatcacc agactggaac
     7261 agaccagaaa cgctgccgta aggagtcacg taacctgcat cacccagttt gaagtcgtaa
     7321 ccggctttca aaccaaagcc ccaagcgtcg gagttggtgc tgccgtcaac gtaagtaccg
     7381 ttgctcatgg ttgcagacag gtcgttgttg aagtgagagt agctcaagct accgtcaaca
     7441 aagacgttgt tcgcgaagtg agcagaagag tagatgtagg cagtctggct gtcttgatcc
     7501 acctgaccag aacggtcatt catatcacct ttagcgaagc ctgcagccgc accgacgatc
     7561 cacttagcgt tgttaccgtc aattttggta tcaacaccga ccatgatgcc gttaacatcc
     7621 tgatcatagt tgatggtgcc gttgtcgccg ttgaagttac caccgaagta gcttacccat
     7681 gcgccgccgt tatcagccag accatgacga gagttggtca gacgagtacc aacggtgtct
     7741 tgttccaggt tccagatatt ggtgttcgca gacgggatgc tcagcgccat gttagcgtag
     7801 tcggtcagct ccatctgttg cagaacaacg gtgttaccac gctgctcagc ctgataggtg
     7861 tatgcaccca ggtcagcttt gttagcagca gagaaggtcg cgttgctgtt tttgtcgttg
     7921 acgtagatca gctcgttgcc gttgtagtca gcgatagaac cagcaccagt agcgttgtcg
     7981 atacgaactt tgtagttacc ggttgcagct gcaacatggt caccgttgtt atcaacttcg
     8041 ttctgaccag cttcgataga agccgtgttg tcgttatcgc cgttaccgtt aatggtcagg
     8101 tgaccatcag agttcattgc gattaagcca taaccgtagt ttgacttggt agtatcgttg
     8161 gtacggtcgt tggtcagatc ggcatcgagt acgaagcggt tgctttcgat gttgaacaca
     8221 cctgcgtgaa tgttaccact ggtatcagta gtgctggtca ggttcagagt atcaacctgc
     8281 agagcattcg ccgcagtgtt acctacatcc aggataccgt cgttagtcac tttgatgttg
     8341 ttagcataca gagcagcatc acctacagcc cagccagcag aaacttcatc agaaatggtt
     8401 acggtactgt tgtcgacagt cagtgaatca gttgcaacat gaccatcttc gttgatgttc
     8461 agcgcagaaa gaccagtcag agagatagag tcagaaatca gcgttgaatc agcaacatta
     8521 acagttgaac cactgttgat gctcagagta tcaatcagag aagttttggt ggtatcccac
     8581 tgtgaaccgg tgttcagggt cacattgaac agaccgctct ggtaaacttc gttaccgatg
     8641 atgtgatcaa attcgctgta ttcagagtcg ctgttttcta caccaacagt tgatgaaggc
     8701 cacaggctgt tagctgcgat gtcgatcaga gttgcagtgg cttcagtatt gattccatat
     8761 gtataggagt catttacacc atcgtcatca gtatcaacac caatctgatg cgcagacatt
     8821 gccgcaccga cccacttgct gccattgttc agggtcagat ccaggcgatc agtaccatcc
     8881 caaccgttag tatctacatc accgtcagca tcacgatagc tatcacgacc ttgtgggaag
     8941 aagttctcat caaaattact tgagaagaat acatcgccca tcagtgtgga attgtcgaat
     9001 gttgcagtgg tttgcatggc gttatcagca tatggatgtg caataacagc taatgcaaca
     9061 tcatcagcat tccacccatc accattgtaa tcactagcat taccagtatt accaaaccaa
     9121 ccagtagtac cttcatcagc ccatgaacca gaggtcacgg tagaatcttt aacagtgatg
     9181 gtgttattaa atacttcgct gctgtttaca ccagtgctga cagtgttgtc gtcgatatct
     9241 tcccactcat aaccctgagt cagcgtaata cctgctacat gagagttatt agagatggac
     9301 agatcgactt cctgatccag agtgatggca gtaccaatgt taaatacatt tacaacatga
     9361 gtgtcagcgg cattattgta agtgccgtta tatgtgtagt gttcgtagtt gtcatcgatc
     9421 gttgaattat caacggtcag cgccagacga tcatggtagt agtcatccgc acgatcagca
     9481 caatcagttg tcatgcactc ggaataaatc ataccgtgaa tagtgctgtt tttgattgtc
     9541 aggctattgg cgttggtatt agtagaaata ccatcgtcca gatagtaggt agaaatcacg
     9601 ccgttaacgg tagcgttatt aatgaccgga tagatatcgc cgttgtaata actatcagcg
     9661 gcatagttat tccagcctac ataaccatca tagtaaacgt catcagcata ggtggcgtcg
     9721 ttgtagtgat ggaaagtatt gtaagtttga ccagaaatat cgttggtcgc tgcattagcc
     9781 tgagaggtga tagccagagt gcaagccaat gctaattgtg atactacaag tttctttttc
     9841 caggagtgca tttgtcatcc ctcctcaggg acgtaataag ttgatccatc aattatcaat
     9901 gcatagaaaa catattatgt ttcgcgttta cgattatgcg gcgttataga ggaatggttc
     9961 aattattctt tcccctaagt ccgattgcgg acatatataa agcacgtgaa cacacacaaa
    10021 gaaagaaata acaaaaacac aatatttcaa tacgttaacg aaagcgttaa ttttaaaaca
    10081 tagaccatga aaatataaaa caaccaaaac agacaaataa tgtatcaata agcaatgaac
    10141 tttaagttat tcactttttg aaaacgtcac caaatcccca aaaacaataa tctatggcta
    10201 taaataaaaa ttaaaaatat tttcagtgaa tatgaaatta ttgctatttc tattacaaag
    10261 atgtcctgac ctctttttca ttctcttctt gtcaacatcg cgacaacttt cgtaaaacac
    10321 acccagcctg cttcaggtat actatgctcc tcgattccac aaacatcagg cagaccatga
    10381 cagatttaat ccaacgccct cgtcgcctgc gcaaatctcc tgcgctgcgc gctatgtttg
    10441 aagagacaac acttagcctt aacgacctgg tgttgccgat ctttgttgaa gaagaaattg
    10501 acgactacaa agccgttgaa gccatgccag gcgtgatgcg cattccagag aaacatctgg
    10561 cacgcgaaat tgaacgcatc gccaacgccg gtattcgttc cgtgatgact ttcggcatct
    10621 ctcaccatac cgatgaaacc ggcagcgacg cctggcggga agatggactg gtggcgcgta
    10681 tgtcgcgcat ctgcaagcag actgtgccag aaatgatcgt catgtcagac acttgtttct
    10741 gtgaatacac ttctcacggt cactgcggtg tgctgtgcga gcatggcgtc gacaacgacg
    10801 cgactctgga aaatttaggc aagcaagccg tggttgcagc tgctgcaggc gcagatttca
    10861 tcgccccttc cgctgcgatg gatggtcagg tacaggcgat tcgccaggcg ctggacgctg
    10921 cgggctttaa agatacggcg attatgtcgt attcgaccaa gttcgcctcc tccttttatg
    10981 gcccgttccg tgaagctgcc ggaagcgcat taaaaggcga ccgcaaaagc tatcagatga
    11041 acccaatgaa ccgtcgtgag gcgattcgtg agtcactgct ggatgaagcc cagggcgcag
    11101 actgcctgat ggttaaacct gccggagcat acctcgacat cgtgcgtgag ctgcgtgaac
    11161 gtactgaatt gccgattggc gcgtatcagg tgagcggtga gtacgcgatg attaagttcg
    11221 ccgcgctggc gggtgctata gatgaagaga aagtcgtgct cgaaagctta ggttcgatta
    11281 agcgtgcggg tgcggatctg attttcagct actttgcgct ggatttggct gagaagaaga
    11341 ttctgcgtta atgatcatta tgccgggtta tccaacccgg catttccccc ttaccccgcc
    11401 cgataaaacg gtttatcccc aagaatcgtc gcccgatgca ttatccgccg ctgtggcagg
    11461 taatccgcat tggcatagtg ctgggttacg cggttatccc aaatcgcaat atcatttggt
    11521 tgccagcacc agcgcacctg aaactccggt ttggtgatgt gggcaaataa aaagcccagc
    11581 aaggcttcgc tctctttctc gctgacatca acaattcgcg tggtaaagcc ttcattcaca
    11641 aacagcgcct gtttaccggt caccggatgc gttcgcacca ccggatgtag caacggtgga
    11701 tttttcgcga ccgcctcacg ccagcgttga tgctcagcct cggttttgcg gtacttgtat
    11761 tccgggaacg atttacggaa atcatgctcc gcacgcaacc cgctcaacag ctggcggaag
    11821 ggaacagaga gcgcctcata tgcggcaata ccactggccc agagcgtatc gccgccggtc
    11881 gagggtaact ctttcgccgc cagaatcgcc cctgcgggtg gcgtttgaat aaatgtcaca
    11941 tcggtgtgcc agttgtcgtt atccggcgga ttatcgttat gggtatccag gacgatgatc
    12001 tcgtcaaccc cttcagcatg agggtaaaca gggtgaatat gcagctcgcc aaaacgctgg
    12061 gccagcgcgc gttgctgctg cggcgtaata gcttgatcgc gcagaaacac cacctgatgg
    12121 cgcagcaccg catggtaaag ctgttcaaac tgattatcgc ttaacgggcg cgttaagtcg
    12181 gcccccgtaa tttgtgcgcc aatatacggc cccagcgggg taatgctcag acgttcactc
    12241 attgtacttc tccatgccag ggcgtcaggc ggcgctgtaa cgcgcgcaga cccagttcta
    12301 aaagaaaggc gataatcgca atcaccgcga tccccgccag caccacgtca gtcgcgagaa
    12361 attcacccgc tgactgaacc ataaatccta aaccgcgcgt tgcggcaatc agctccgccg
    12421 ccaccagcgt agaccagccc acacccagac caatgcgtaa tccggtgagg atttccggca
    12481 gcgcgccggg caaaatgaca aaccacagca cctgcgcacg gctggcaccc agcgactggg
    12541 cggcacgaat gcgaacctgc tgcgcgcttt tcacccccgc cagcgccgac atcgccaccg
    12601 gcgcaaaaat cgctaaatag atcagtaaga tcttcgaggt ttcgccaata ccaaaccaga
    12661 tcaccatcag cggcaaataa gccagcggcg gcaccggacg ataaagctcg attatcggat
    12721 ccagaatgcc gcgcaccgtc gggcttagcc ccatggcgat cccgaccgga ataccgatca
    12781 acaccgccgc gagtagcgcc agcaaaatgc gcgtcaggct ggctgccaga tgctgccaca
    12841 gcgtggcgtc cataaatccc tgcggcccgg caatggtgag cagttttgcc agtacctgtt
    12901 gcggtggcgg taaaaacagc gggctaatca gttgcagcgc cgcgactgcc caccatacag
    12961 tgagcaaaac cgctaacgta ccaatgctga aggtcacctg acgcgagagc ggccagcgcc
    13021 atttcagccg atgcgaatgc agtttttcat taatgagcac actcatgaga acgcctcccg
    13081 ttgctcaaat acgcggctta aaacatattc gcgcatggcg atgaattgtg gatcggactt
    13141 gatgctgcgg ctcgactctc ccgcaacaaa gcggcgagca aagttgagcg gcagccgctc
    13201 cagcacacgg cctgggccgg gtgaaagcag aaccagttca gtcgccataa acaccgcttc
    13261 ttctatatcg tgggtaatca acagcacctg ctttcccgtc tcctgccaga gtttcagcag
    13321 cagggtttgc atctggtcgc gggtgaaggc gtccagcgca ccaaacggtt cgtcgagtaa
    13381 taacagctgg ggatttgccg ccaacgcgcg ggcaatcccc acccgctgac gttgaccacc
    13441 ggaaagctgc cagatgtagc gtttttctgc gccttccagc cccacttttt tcaccatctg
    13501 gtgtgcgatt tccagtcgct gcattttctc tacacctgcc agttgcaggc cgaacaccac
    13561 gttgtctacc acattacgcc acggcaacag cccttcattc tgaaaaacca cgccccgctc
    13621 tgctcccggt ccctcaatgc acttacccgc cagttgaatg ctgccatgct gataaggcac
    13681 aaaaccggca atcagattca gcaaggtggt tttgccgcag ccagacggcc ccagaactac
    13741 cagaagctcg ccgctttcca gcgtcaggtt gatatcttcc agtgccggtt tgccgccata
    13801 atcggcgtaa agatgagaaa tttgcagcat ctgcgcctcc ttttattgca cgaagcgcga
    13861 ggtaacgtac tggctgtaat cattcgccac ggcggggact ttgccctgct ctttcaaaaa
    13921 ctgcgcggtg tcgatgatcg ctttgttcac cggtccggtc agttctgccg tttgctgctg
    13981 cggcgtcaga taggtattcc ccttcaccag ccccggaacg tcaccttcag gcacgccgct
    14041 taaccgcgcc agtttgctga tgttttccgg ctgtttcagc cacgcgtctg ggttagcaat
    14101 gtacggttgc tgagcatcga tggcgctttt agcgaacgct ttcacgacct caggatgttt
    14161 ctcggcaaaa tctttccgca cgacccagac gtccagcgtc ggcgcgcccc actgcccgac
    14221 ctgttcagaa tcggtcagca ccttgccgtc tttttccagc gcgttaaccg ccggtgccca
    14281 gacataagca ccatcaatat ctccccgctg ccaggcggcg ataattgcgg gcggctgcag
    14341 gttcacaatc tccacttgcc caggtttaat accccagtgt ttcagcgccg ccagcaggct
    14401 gtagtgggtg gtggagataa acggtacggc gatgcgcttg ccaatcagat cttccggttt
    14461 gctgatagtt ttcttcacca ccagcgcttc ggagttaccc agttttgacg ccagtaagaa
    14521 gacttcaatc ggcacctgtt ggctggctgc caccgctaat gggctggaac cgaggttacc
    14581 gatttgcacg tcgccagaag ccagcgcccg cacgatgctg gctccgctgt caaacttacg
    14641 ccagtcaacg ctcgccccgc tctctttagc aaaggtgttg tcggcctgag ccactttcgc
    14701 cggttcggct gaagtttgat acgccacagt gacgttcacc gcctgtgcct gaaaagcgat
    14761 gaatgccagt gcggcaagaa gtgtgttacg cgatgaaatt gccatgattg tctgctcccc
    14821 tgtcttgtta tgggagcagt attcaggaat aaaaacattc attaaaagaa ttagttgtta
    14881 tcgcacagat gattttattc ttagcgaaaa agtggcaaag gaaagattaa atatgcaatt
    14941 acacccactc tgacaattca gtaagttact gcacttcatt ttattaatgc ataaacaagc
    15001 agattataat gtcttttatt ttattaacag caatacatat
//
LOCUS       DAZGHF010000118        14367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGHF010000118 DAZGHF010000000
VERSION     DAZGHF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.119"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            9..836
                     /locus_tag="ACHK3A_003777"
     CDS             9..836
                     /locus_tag="ACHK3A_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO8741804.1"
                     /translation="MLLIPCIGNAQEIKIDESWLHQSLNVIGRTDSRFGPRLTNDLYP
                     EYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFSIDKLTGLNL
                     AFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANIYAKYQWQNY
                     GAANENEWDGYRFKIKYSIPLTHLFGGRLVYNSFTNFDFGSDLADKSHNNKRTSNAIA
                     SSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGWGTYTTIGYQF"
     gene            complement(1053..3485)
                     /gene="metL"
                     /locus_tag="ACHK3A_003778"
     CDS             complement(1053..3485)
                     /gene="metL"
                     /locus_tag="ACHK3A_003778"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO8741805.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3488..4648)
                     /gene="metB"
                     /locus_tag="ACHK3A_003779"
     CDS             complement(3488..4648)
                     /gene="metB"
                     /locus_tag="ACHK3A_003779"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO8741806.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRVALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4925..5242
                     /gene="metJ"
                     /locus_tag="ACHK3A_003780"
     CDS             4925..5242
                     /gene="metJ"
                     /locus_tag="ACHK3A_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO8741807.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5302..5910
                     /gene="yiiX"
                     /locus_tag="ACHK3A_003781"
     CDS             5302..5910
                     /gene="yiiX"
                     /locus_tag="ACHK3A_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO8741808.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGELSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGKQQKLKEFDLSNPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(5971..6183)
                     /gene="rpmE"
                     /locus_tag="ACHK3A_003782"
     CDS             complement(5971..6183)
                     /gene="rpmE"
                     /locus_tag="ACHK3A_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO8741809.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            6386..8584
                     /gene="priA"
                     /locus_tag="ACHK3A_003783"
     CDS             6386..8584
                     /gene="priA"
                     /locus_tag="ACHK3A_003783"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO8741810.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            8740..9765
                     /gene="cytR"
                     /locus_tag="ACHK3A_003784"
     CDS             8740..9765
                     /gene="cytR"
                     /locus_tag="ACHK3A_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO8741811.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            9857..10816
                     /gene="ftsN"
                     /locus_tag="ACHK3A_003785"
     CDS             9857..10816
                     /gene="ftsN"
                     /locus_tag="ACHK3A_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO8741812.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKIAGHTNCIRLAAGG"
     gene            10909..11439
                     /gene="hslV"
                     /locus_tag="ACHK3A_003786"
     CDS             10909..11439
                     /gene="hslV"
                     /locus_tag="ACHK3A_003786"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO8741813.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            11449..12780
                     /gene="hslU"
                     /locus_tag="ACHK3A_003787"
     CDS             11449..12780
                     /gene="hslU"
                     /locus_tag="ACHK3A_003787"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO8741814.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            12847..13773
                     /gene="menA"
                     /locus_tag="ACHK3A_003788"
     CDS             12847..13773
                     /gene="menA"
                     /locus_tag="ACHK3A_003788"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO8741815.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13866..14351
                     /gene="rraA"
                     /locus_tag="ACHK3A_003789"
     CDS             13866..14351
                     /gene="rraA"
                     /locus_tag="ACHK3A_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO8741816.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         3502 a         3829 c         3800 g         3236 t
ORIGIN      
        1 ctttatccat tctattaata ccttgcattg gaaacgctca ggaaattaaa attgatgaaa
       61 gctggttaca tcaaagcttg aatgtcattg gtcgcacaga ctctcgcttt ggcccaagac
      121 tgactaacga cctctaccct gaatatactg tagcaggaag aaaagactgg tttgattttt
      181 atggttatgt tgatctacca aaattctttg gcgtcggcag tcactatgat gttgggatct
      241 gggatgaggg ctcaccacta tttacggaaa tagaacctcg gttttccatt gacaaattga
      301 ccggattaaa tcttgcgttc ggccctttta aagaatggtt cattgcaaac aactatgtct
      361 atgatatggg tgacaaccag tcatcccggc aaagtacatg gtatatgggg cttggtacag
      421 atatcgacac gggtctacca attaagcttt ctgccaatat atacgccaag tatcagtggc
      481 aaaactatgg tgccgctaat gaaaatgaat gggacggata tcgattcaaa ataaaatata
      541 gcattcctct tacccattta ttcggagggc gattggtata caatagtttt actaactttg
      601 attttggctc cgatcttgcg gacaaatcac acaataataa acgaaccagt aatgctattg
      661 cttcaagcca tatcctttcc ctgctatatg aacactggaa atttgcattt acactacgtt
      721 attttcacaa cggtggacaa tggaatgctg gggagaaagt taacttcgga gatggtccat
      781 ttgaattaaa aaacacagga tggggaacct atactactat tggttatcaa ttttaaatca
      841 ggtgatgttt tagatatttt tacttaatgc cattttgtaa ttgtcataaa taacttcacc
      901 attcacactg tgaattcatc gctcaacaca aaagccccct tattaccggg gcactgacca
      961 aaggcaaaat gacaatcaat caccacctcc gcaggaggtg gtttagaggt gacaacaaaa
     1021 gctggcaact gccagctttt gttcaataat cactacaaca actgcgccag acggttaata
     1081 tccgactgaa tcgccccggc ggtgacgtcg cgccctgcac ctggcccgcg gatcaccaac
     1141 ggattatcgc gataccagcg gctttcgatg gcaaagacgt tatcgcacgg tagtagtgat
     1201 gccagcggat ggtcttctcg caccgcttcc acgcctactc gcgctttgcc gttggcatcg
     1261 aaacgcgcca cgtagcgcaa caccagcccc atttcacggg ccgcttccaa ccgttgtacc
     1321 atctgctcat taagttcatc gccattttca aagaaatggt cgatgcttcc accttcgcaa
     1381 tgggttggca ccagtgattc aacacgcacc tggtccggtt caatgttgta acccgcttca
     1441 cgcgccagaa tcaccagctt gcgcatcaca tctttgccgg agaggtcatc acgcgggtca
     1501 ggttcagtta agccctgctg ccacgcctga tccaccagct cagtaaacgg cacgctaccg
     1561 tcgaattgca ggaacagcca ggagagcgtg ccggagaaga tcccgctgat cgacaaaata
     1621 gtgtcgccgc tgtcgatcag atcgcgcacg gtatggttga tcggcaaacc tgcgccgacg
     1681 gtggcgttgt acagccagtg acgcccggtt ttttcgaacg cgtcgtggat ctggcgatat
     1741 ttattgctgt cgctcgctcc cgccagtttg ttggcactga tgacgtggaa accgtggctg
     1801 gcgaaatcga gatactgatc agcaagctgc tggctggcag taacgtccag cacaactaaa
     1861 tcatcatacg gatgggcacg catccacagg aatagcgact cttcatcctg ttcgacggct
     1921 tcatcattaa agaaggctag cgcacggctg gcgtccagcc cgtcatagct caacaggctg
     1981 cgacggctgt ccaccacgcc tgccagcaca aattcaaagc cggtgcgcgc cgagagcgtg
     2041 ctctgctcac gggcgaacag ttccagccag cgggaaccga tatttccctt accgaacaat
     2101 accaggccga tgcgtttttc tgcgcggaag acggactgat gcagcccctg aatcaggctt
     2161 tcggtcgggc cggtgcgcag tactgccacc aggctgatgc cgtcatcgga ctgccaggta
     2221 aactcgaccg gctggccttt cagttgctgc cagaagcggt ggcaatgcag cgggttacgg
     2281 gtgacgcccg ccccaaccat cgccaccagc gccagcccct gacgcagacg cagttcgccg
     2341 ggtaatcctg cttcgtcgag gattttcagc gcactgtcgg ccacttccga ggtgtagcaa
     2401 aattgcagca actggcgatc gttatgcacg ccaactgcca gtgggcgcac ctgagcacgt
     2461 ttcaggattt ggtcgatctc tttatgcgcc agtttaaaat cctgactggc gggcacctga
     2521 aactcaatca aacagacatc atcgtggctg gtgacaatac gcgcaccagt accggaggcc
     2581 agcacgcgtt caatgcgcgt ggaaccttga tccggcgtgt agctacagcg cagctgcagg
     2641 tcgatttcac taccggatac tggctgtaaa gtacgggcgt gaagaacggg ggccgccaga
     2701 cgcgccagtt cgctggcttc atcaagacgc agcaatggca gcagacaggc atctttcact
     2761 ttacgcgggt cggcactata taccccggcg acatcgctcc agatggttac gcgggaaaca
     2821 cccgccagcg caccgatttg tgtcgcggaa tagtcggaac cgttacgccc cagcagcacc
     2881 gtttcaccgg cgttgttacg gctgataaac ccggtcacca ccagacgttt gcccggatgt
     2941 tgtaccagta gctgttgcag caacgggtaa gaaagccctt catcaacctg cggctgcgcg
     3001 gcgcgttcag cgcgtaaaaa ctcgcgggca tcaagccagg ccgctggcag cccttgttga
     3061 ttaagtaccg cagacatcag gcgcgccgac cacacttcac cgtggcccac cacttccgca
     3121 tacaccgcat cattaatacc gctgtcgagc agcgccgcga ggcgttccag atcgctgaca
     3181 aaggcgctaa tgaggctatc ggcttcttcg gcgggtagca ggccgctaat cagatcgcac
     3241 tgataacgac gtagcgtttg ttgaacctga tgcgcagaga gacgatcggt ctggcttagt
     3301 ttcaaccagt taatcaactg gttagtggtg ctaccggcgg cggaaaccac catcatatcg
     3361 tcaggctgag agtactccgc cataatgccc gcgacacgca aataacactt cacatcagcc
     3421 agactactgc caccaaattt atgcagttga cgacctttcg cccctgcctg cgcaatcaca
     3481 ctcattttta ccccttgttt gcagcccgga agccattttc caggtcggca attaaatctt
     3541 cgccatcttc aataccggtg gagatacgta gcagcgtctc ggagatcccg gcggcagcac
     3601 gcgcttctgg cgccatgcct gcatgtgtca tggttgcggc gtgagagatt aaactttcca
     3661 ctccccctaa tgattccgcc agcgtaaaca acgacaaccc gcccaggaaa cgacgcagcg
     3721 tctgctcatc gccatccagt tcaaaactca acattgcgcc aaagcctttt tgctggcgcg
     3781 cggcaatttc atgcccctga ttttccggca gcgacgggtg atacagtttt ttcaccaacg
     3841 gctgggtttg cagatatttc acaatcgcct gcgcgttgcg ctgcgccagc tccatacgcg
     3901 gcaccagcgt tcgcaacccg cgcagcagca gatagctgtc aaacgcgccg cccgtcacgc
     3961 caatattgtt tgcccaccag gcaagttcag tgacaacgtc cgggtcttta gcaatcacta
     4021 cgccggccac cacgtctgag tgaccgttca gatatttcgt acatgaatgc aacaccagat
     4081 cggcacctaa tgccagcgga ttttgtaatg ccgggcttaa gaaggtgtta tccaccacgc
     4141 tcaccgcccc gacttccctt gccagatggc agattttcgc aatatccacg acgcgtaaca
     4201 atggattact tgggctttct accagtacca gtttgggttt ttctgccagc gctacccgta
     4261 atgcctgttc atcgccttga tcaacaaaca acacgcgata gcaaccgcgt ttcgccagac
     4321 tgtcgaacag gcgatagctg ccgccatagc agtcgtgcgg cgcaaccagc agatcgcccg
     4381 gtttcaaaaa gacggtcgtt accagatgaa tcgcggacat gccggtatta gtaagtacgg
     4441 caccagcacc gccttccagt tctgccagcg cacgctgaac cacatcgcgc gttgggttgc
     4501 cgcgccgcga gtaatcatgc gcgcgcggtt cattaaatcc ggtaaagtta taggtgctgg
     4561 aaagatggat cggtgggaca acgcaaccat actgttcgtc gtcatttaac ccgctacgca
     4621 ctgcgatggt ggcctgttta cgcgtcatgt gatgaagttc cctgggcttg gtcggtgaaa
     4681 tgtcaggcac cagagtaaac attgtgttaa tggacgtcaa tacatctgga catctaaact
     4741 tctttgcgta tagattgagc aaatcccaaa tagccgttaa aattatatgc attatcacgc
     4801 cgacaggtgc attacacgat gtcacggtag cgcctgtacg gtaaactatg cgggtttacg
     4861 gtcagtaccc acataaattg tgtggtctgg tctcaattta ttgacgaaga ggattaagta
     4921 tctcatggct gaatggagcg gcgaatatat cagcccatac gctgagcacg gcaagaagag
     4981 tgaacaagtc aaaaagatta cggtttccat tcctcttaag gtgttaaaaa tcctcaccga
     5041 tgaacgcacg cgtcgtcagg tgaacaacct gcgtcacgct accaacagcg agctgctgtg
     5101 cgaagcgttt ctgcatgcct ttaccgggca acctttgccg gatgatgccg acctgcgtaa
     5161 agagcgcagc gacgaaatcc cggaagcggc aaaagagatc atgcgtgaga tggggattaa
     5221 cccggagacg tgggaatact aaagcggaaa agagcggcgc ggagttaacc agccgctttt
     5281 ttcatacatg gacgtttaac gatgaaaatc aggctactga tcctcagcct gctggtttct
     5341 gtgcctgcct ttgcctggca gccgcaaact ggcgacatca tctttcaaat ctctcgctca
     5401 tcgcaaagta aagcgatcca actggcgacc cattccgatt acagccacac cggtatgctg
     5461 gtgatacgca acaaaaagcc ctacgttttt gaagcggtcg gcccggtaaa atacacccca
     5521 ctcaagcagt ggatcgccca tggtgaaaag ggcaaatacg ttgttcgccg cgttgaaggc
     5581 gaactgagtg ttgaacaaca gcaaaaactg gcgcaaacgg caaaacgtta tctcggtaaa
     5641 ccgtatgact ccagcttctc gtggagcgac gatcgccagt actgttcgga agtggtttgg
     5701 aaggtttatc agaacgcgct gggaatgcgc gtgggcaagc agcaaaagtt gaaagagttc
     5761 gaccttagta acccgcaggt tcaggcaaag ctcaaagaac gctacggcaa aaatattccg
     5821 cttgaggaaa cggtggtctc gccacaagcc gttttcgatg cgccacaact caccacggtc
     5881 gccaaagaat ggccgctgtt ttcgtggtaa tacgaccaaa attcaggcac aaaaaaagcg
     5941 ccgtgcggcg cttttttcgg aaatccggtc ttatttgctg cccgggatgt tgaaacgctt
     6001 gttgaagcgg tcaacacggc caccggtagc aacatcacgc tgtttgccag tgaagaacgg
     6061 gtggcacttg ctgcacacgt cgaggttcag gtcatgacca acggtggagc ggattttcat
     6121 tacgttaccg caagagcagc tagcagtaat ttcttcgtat ttcgggtgaa tatctttttt
     6181 catgggaaaa cctcggttta aggccgcgtc gctcttccag ccctaacgcc agacaccacg
     6241 cgatgttaaa agtatagctt caatacgatc atttcgtatg aaggcgcgaa atcatacaga
     6301 aattaaccag cgtatgcaaa ctgatccgca ctcttctacg gcaatgtgta tactaaccca
     6361 ccgaatttca agtcaggatg atgctatgcc cgttgcccac gttgccttgc ccgttccgct
     6421 tccccgtacc tttgactatc tgctgccaga aggcatgacg gtcaaagccg ggtgtcgcgt
     6481 gcgcgtgccg tttggcaaac agcaggagcg catcggggtt gtggtgtcag ttagcaatgt
     6541 cagcgagctg ccgcttaacg agctgaaagc ggtagttgaa gtgctggacg ttgagccggt
     6601 atttactcac tccgtctggc gattactgct ctgggcggca gattactatc atcatccgat
     6661 tggcgatgtg ctgtttcatg ccttgccgat tttactacgc caggggcggc ctgcggcgaa
     6721 cgcgccgatg tggtactggt ttgccactga acaaggtcag gcggtggatc tgaacagcct
     6781 gaaacgctca cccaagcaac aacaggcgct ggcagcgtta cggcaaggca aaatctggcg
     6841 cgaccaggtt gccacgctcg aatttaatga tgccgcgttg caggcgctgc gcaaaaaagg
     6901 tctgtgtgat ttagcaagtg aaacaccaga gtttagtgac tggcgaaaga attatgccgt
     6961 ttctggtgag cggttgcgat tgaatactga acaggccacc gccgttggcg caattcatag
     7021 tgcggcggat accttctctg cctggctgct ggcgggcgtt accggttccg gtaaaacgga
     7081 ggtttatctc agtgtactgg aaaacgtgct cgctcagggc aaacaggcgc tggtgatggt
     7141 gccggaaatc ggcctgacgc cgcaaactat cgcccgtttt cgtgaacgtt ttaatgcgcc
     7201 agtggaagtt ctgcattccg gcctgaacga cagcgagcgt ctttcggcgt ggttgaaagc
     7261 gaaaaatggt gaggcggcga ttgtgatcgg cacccgctcc gcgctgttta cgccgtttaa
     7321 aaatctcggt gtgattgtca ttgatgaaga gcacgacagt tcctacaagc agcaggaagg
     7381 ctggcgctat catgcccgcg acctggcggt ttatcgtgcg cacagcgagc aaatcccgat
     7441 tattcttggc tccgcaacac ccgcactgga aacgttatgc aacgtccagc agaaaaaata
     7501 ccgcctgctg cgcctgacgc gtcgggcggg gaatgcgcgt ccggcaattc aacacgtgct
     7561 ggatttaaaa ggtcagaagg tgcaggcggg gctggctccg gcgttaatca cccgtatgcg
     7621 ccagcattta caggccgaca accaggttat cctctttctt aaccgccgtg gctttgcgcc
     7681 tgccttgctg tgccacgact gcggctggat tgccgaatgt ccacgttgcg atcactacta
     7741 cacgctgcat caggcgcaac agcatttgcg ctgtcaccac tgtgacagcc aacgcccggt
     7801 gccgcgccag tgcccttcct gcggttccac gcacctggtg ccggtggggc tgggcaccga
     7861 acagcttgaa cagacgcttg cgccgctgtt ccctggtgtg cccatttctc gtatcgaccg
     7921 cgataccacc agccgcaaag gagcgctgga acaacaactg gcggaagtgc atcgcggcgg
     7981 cgcgaggatc ttgatcggca cgcaaatgct ggcgaaaggt caccactttc cggatgtgac
     8041 gctggtggcg ttactggacg tagacggcgc gctgttttct gccgattttc gctcggcaga
     8101 gcgtttcgcc cagctttaca cccaggtcgc cggtcgtgcc gggcgtgcgg gtaaacaggg
     8161 cgaagtggtg ctgcaaacgc atcatccaga acacccgcta ttgcaaacgt tgctctataa
     8221 aggctatgac gcctttgccg aacaggcact ggctgagcga cgcatgatgc agctgccgcc
     8281 gtggaccagc catgtgattg tgcgtgcgga agatcataac aatcagcacg cgccgctgtt
     8341 cctgcaacaa ctgcgtaatc tcatcctctc cagcccgctg gcagacgaca aactgtgggt
     8401 tctcggtccc gttccggcac tggcacctaa acgtggcggc cgctggcgct ggcaaatttt
     8461 gttgcagcac ccttcccgcg tgcgattaca gcacatcatc aatggtacgc tggcgcttat
     8521 taatacaata ccggattccc gtaaggtgaa atgggtgctg gatgtcgatc ccatcgaggg
     8581 ctaatgcgct cacgatgcga ggcggatcga aaaattcaat attcatcaca cttttcatga
     8641 aaattctgta accgttttca cgcgctatct gctaaaaatg ttgccgatgt gaagtaaaca
     8701 tggatgtagt acgcctgacg tgccaggcga ggagtgagtg tgaaagcgaa gaagcaggaa
     8761 actgccgcga ccatgaaaga cgttgccctc aaggcaaaag tctctacagc gactgtctcc
     8821 cgagcattaa tgaatcccga taaagtctcc caggccaccc gtaatcgggt tgaaaaagcg
     8881 gcccgggaag tgggttattt accgcagcct atggggcgca acgtcaagcg taatgaatcc
     8941 cgcactattc tggtgattgt cccggatatc tgcgatccct tctttagcga aattattcgc
     9001 ggtatcgaag ttacggcggc aaatcacgga tatctggtgc tgattggcga ctgtgcgcat
     9061 caaaatcagc aggaaaaaac ctttatcgat ttgatcatca ccaagcaaat tgatggcatg
     9121 ttgctgctgg gttcaaggct gccgtttgat gccagcattg aggaacagcg taatctgccg
     9181 ccgatggtga tggcgaacga atttgcaccg gaactggagc tgcctaccgt tcatatcgac
     9241 aatctgaccg ccgcatttga tgcagtaaat tatttatatg agcaagggca taatcggatt
     9301 ggctgtatag ccggtcccga agagatgccg ctgtgtcact accgcctgca aggctatgtc
     9361 caggcgctgc gtcgctgcgg cattatggtt gatccgcaat acatcgcccg tggcgacttc
     9421 accttcgaag ccggaagcaa agcgatgcag cagttgcttg atcttccaca accgcctact
     9481 gctgtcttct gccatagcga tgtgatggcg ctcggcgcac tttctcaggc aaaacgccag
     9541 gggctgaaag tcccggaaga cctttccata atcggttttg ataacatcga cctgacgcaa
     9601 ttttgtgatc cgccgctgac aaccatcgcg cagccgcgtt acgaaatcgg tcgggaagct
     9661 atgctgttat tgcttgatca aatgcagggg caacacgttg gcagtggctc tcgtttaatg
     9721 gactgcgaac ttatcatccg gggatcaaca cgcgcgttac cttaaagtaa acctttaaga
     9781 ctgccgtgac tggtcaaagg cccgccgctt aagtaacatg gcgggctgac gaacgaataa
     9841 atacagcgaa acgatagtgg cacaacgaga ttatgtacgc cgcagccaac cggcaccttc
     9901 gcggcgaaaa aagagcacct cacggaaaaa gcaacgaaat ctgcctgcgg tttctcccgc
     9961 tatggtcgct attgctgccg ccgttcttgt gacctttatc ggtggtctgt acttcattac
    10021 gcatcacaag aaagaagagt ccgagacact gcaaagccag aaagtgaccg gaaacggact
    10081 accaccaaaa ccagaagaac gctggcgcta catcaaagag ctggaaagtc gccagccggg
    10141 tgtgcgtgcg cctacagaac cttctgccgg tggtgaagtg aaaacgccgg agcaactgac
    10201 accagaacaa cgccagcttc ttgagcaaat gcaggctgat atgcgccagc agccaacgca
    10261 gctggttgaa gttccgtgga atgaacagac gccggaacag cgtcagcaaa cgctacagcg
    10321 ccagcgtcag gcgcagcaat tagcggagca gcaacgcctg gctcagcagt cgcgtacgac
    10381 tgaacaaagc tggcagcagc agacgcgtac gtcgcaagcc gcgccggtac aggcgcaacc
    10441 gcgccagtct aaaccggctt ctactcagca gccgtaccag gatctgctgc aaacccctgc
    10501 gcacacgacc gcgcagtcga agccgcagca agctgcgccg gttgctcgtg ccgctgacgc
    10561 gccaaaaccg acggcggaga aaaaagacga acgccgctgg atggtgcagt gcggttcgtt
    10621 cagaggcgcg gaacaggcag agacggtacg tgctcagctg gcgttcgaag gctttgactc
    10681 aaaaattacc accaacaatg gctggaatcg tgtggtgatt ggcccggtga aaggcaaaga
    10741 gaacgcagac agcaccctca atcgcttgaa gatagcgggt catacaaact gcattcggct
    10801 cgccgccggg ggttgaaacc ctcaaaatcc cccccatcta taattgcatt atgccccgta
    10861 cttttgtacg gggtttgtac tctgtattcg taaccaaggg gtcagctcgt gacaactata
    10921 gtaagcgtac gccgtaacgg ccatgtggtc atcgctggtg atggtcaggc cacgttgggc
    10981 aataccgtaa tgaaaggcaa cgtgaaaaag gtccgccgtc tgtacaacga caaagtcatc
    11041 gcgggctttg cgggcggtac tgcggatgct tttacgctgt tcgaactgtt tgaacgtaaa
    11101 ctggaaatgc atcagggcca tctggtcaaa gccgccgttg agctggcgaa agactggcgt
    11161 accgatcgca tgctgcgcaa acttgaagca ttgctggcag tcgcggatga aactgcatcg
    11221 cttatcatca ccggtaacgg tgacgtggtg cagccagaaa acgatcttat tgctatcggc
    11281 tccggcggcc cttacgccca ggctgcggcg cgcgcgctgt tagaaaacac tgaacttagc
    11341 gcccgtgaaa ttgctgaaaa ggcgttggat attgcaggcg acatttgcat ctataccaac
    11401 catttccaca ccatcgaaga attaagctac aaagcgtaag gatctcccat gtctgaaatg
    11461 accccacgcg aaatcgtcag cgaactggat aagcacatca tcggccagga caacgccaag
    11521 cgttccgtgg cgattgctct gcgtaaccgc tggcgtcgca tgcagctcaa cgaagagctg
    11581 cgccatgaag tgaccccgaa aaatatcctg atgatcggcc cgaccggtgt cggtaaaact
    11641 gaaatcgccc gtcgcctggc taagctggcg aatgcgccgt tcatcaaagt tgaagcgacc
    11701 aaattcaccg aagtgggcta tgtcggtaag gaagtggatt ctattattcg cgatctgacc
    11761 gatgccgccg tgaaaatggt acgcgtccag gctatcgaga aaaaccgtta tcgcgctgaa
    11821 gaactggcag aagaacgtat tctcgacgta ctgatcccac cggctaaaaa taactgggga
    11881 cagaccgaac agcagcagga accgtccgct gctcgtcagg cattccgcaa aaaactgcgt
    11941 gaaggccagc ttgatgacaa agaaatcgag atcgatcttg ccgcagcacc gatgggcgtt
    12001 gaaattatgg ctcctccggg catggaagag atgaccagcc agctgcagtc catgttccag
    12061 aacctgggcg gtcagaaaca aaaagcgcgt aagctgaaaa tcaaagacgc catgaagctg
    12121 ctgattgaag aagaagcggc gaaactggtg aacccggaag agctgaagca agatgctatc
    12181 gacgctgttg agcagcacgg gatcgtgttt atcgacgaaa tcgacaaaat ctgtaagcgc
    12241 ggcgagtctt ccggtccgga tgtttctcgt gaaggcgttc agcgtgacct gctgccgctg
    12301 gtagaaggtt gcactgtttc caccaaacat gggatggtca aaactgacca cattctgttt
    12361 atcgcttctg gcgcgttcca gattgcgaaa ccgtctgacc tgatcccgga actgcaaggt
    12421 cgtctgccaa tccgcgttga gctgcaggca ctgaccacca gcgacttcga gcgtattctg
    12481 accgagccga atgcctctat caccgtgcag tacaaagcac tgatggcaac tgaaggcgta
    12541 aatatcgagt ttaccgactc cggtattaag cgcatcgcgg aagcggcatg gcaggtgaac
    12601 gaatctaccg aaaacatcgg tgctcgtcgt ttacataccg ttctggagcg tttaatggaa
    12661 gagatttcct acgacgccag cgatttaagc ggtcaaacta tcactattga cgcagattat
    12721 gtgagcaaac atctggatgc gttggtggca gatgaagatc tgagccgttt tatcctataa
    12781 tcgcgttcaa tcattttcat cattgtttga tggggctgaa aggccccatt tttattggcg
    12841 cgtattatga ctgaacaaca aattagccga actcaggcgt ggctggaaag tttacgacct
    12901 aaaaccctcc ccctcgcctt tgctgcaatt atcgtcggga cggcgctggc atggtggcaa
    12961 ggtcacttcg atccactggt cgccctgctg gcattgatta ccgccgggct attacagatc
    13021 ctttctaacc tcgccaatga ttacggcgat gcggtaaaag gcagcgataa acctgaccgc
    13081 attgggccgc tacgcggcat gcaaaaaggg gtcattaccc agcaagagat gaaacgggcg
    13141 ctcattatta ccgttgtgct catctgtctt tccgggctgg cactggttgc agtggcgtgc
    13201 catacgctgg ccgattttgt cggtttcctg attcttggcg ggttgtcgat cattgccgct
    13261 atcacctaca ccgtgggcaa tcgtccttat ggttatatcg gtttgggtga tatttccgta
    13321 ctggttttct ttggctggtt gagcgtcatg gggagctggt atttacaggc tcatacattg
    13381 attccggcac tgatccttcc ggcaaccgca tgcggcctgc tggcaacggc agtactgaac
    13441 attaataacc tgcgtgatat caatagcgac cgcgaaaacg gcaaaaacac gctggtggtg
    13501 cgcttaggtg cagtaaacgc gcgtcgttat catgcctgcc tgctgatggg ctcactggtg
    13561 tgtctggcgc tgtttaatct cttttcgctg catagcctgt ggggctggct gttcctgctg
    13621 gcggcaccat tactggtgaa gcaagcccgt tatgtgatgc gggaaatgga cccggtggcg
    13681 atgcgaccga tgctggaacg cactgtcaag ggagcgttac tgactaacct gctgtttgtt
    13741 ttagggatat tcctaagcca gtgggcagca taactgacaa atatcaatta acaattgatg
    13801 attttgccaa cagcctacat agcgcgatat actgaaaatt ctcgcagcaa ctgaatgtta
    13861 agcctatgaa atacgatact tccgagcttt gtgacatcta tcaagaagat gttaacgtcg
    13921 tggaaccgct gttctccaac tttggcggac gggcgtcgtt tggcggacaa ataatcacgg
    13981 taaaatgttt cgaggacaac gggttgctgt acgatctgct cgaacagaat ggccgtggtc
    14041 gtgttcttgt cgtcgatggc ggtggttctg ttcgtcgcgc actggtcgat gctgaactgg
    14101 cgcgtctggc agtacaaaat gaatgggaag gtctggtcat ttacggcgcg gtgcgtcagg
    14161 tagatgacct ggaagagctg gatatcggca tccaggcaat ggccgcaatt ccggttggtg
    14221 ccgctggcga aggcattggc gaaagcgatg tccgcgtcaa ttttggcggt gtaaccttct
    14281 tctccggcga ccatctttac gccgacaata ccgggattat tctttcagaa gatccgctgg
    14341 atattgaatg ataaaaaagg caccgca
//
LOCUS       DAZGHF010000119        14366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGHF010000119 DAZGHF010000000
VERSION     DAZGHF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.120"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            114..470
                     /locus_tag="ACHK3A_003790"
     CDS             114..470
                     /locus_tag="ACHK3A_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO8741817.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(521..1417)
                     /gene="yhaJ"
                     /locus_tag="ACHK3A_003791"
     CDS             complement(521..1417)
                     /gene="yhaJ"
                     /locus_tag="ACHK3A_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO8741818.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1522..2223
                     /locus_tag="ACHK3A_003792"
     CDS             1522..2223
                     /locus_tag="ACHK3A_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO8741819.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2246..2410
                     /gene="yhaL"
                     /locus_tag="ACHK3A_003793"
     CDS             2246..2410
                     /gene="yhaL"
                     /locus_tag="ACHK3A_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO8741820.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2542..3852)
                     /gene="cyuA"
                     /locus_tag="ACHK3A_003794"
     CDS             complement(2542..3852)
                     /gene="cyuA"
                     /locus_tag="ACHK3A_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO8741821.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYEIISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3880..5211)
                     /locus_tag="ACHK3A_003795"
     CDS             complement(3880..5211)
                     /locus_tag="ACHK3A_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8741822.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGVLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5486..6850)
                     /gene="tdcG"
                     /locus_tag="ACHK3A_003796"
     CDS             complement(5486..6850)
                     /gene="tdcG"
                     /locus_tag="ACHK3A_003796"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO8741823.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6922..7311)
                     /locus_tag="ACHK3A_003797"
     CDS             complement(6922..7311)
                     /locus_tag="ACHK3A_003797"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO8741824.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7325..9619)
                     /gene="tdcE"
                     /locus_tag="ACHK3A_003798"
     CDS             complement(7325..9619)
                     /gene="tdcE"
                     /locus_tag="ACHK3A_003798"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO8741825.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9653..10861)
                     /gene="tdcD"
                     /locus_tag="ACHK3A_003799"
     CDS             complement(9653..10861)
                     /gene="tdcD"
                     /locus_tag="ACHK3A_003799"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO8741826.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10887..12218)
                     /gene="tdcC"
                     /locus_tag="ACHK3A_003800"
     CDS             complement(10887..12218)
                     /gene="tdcC"
                     /locus_tag="ACHK3A_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO8741827.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12240..13229)
                     /gene="tdcB"
                     /locus_tag="ACHK3A_003801"
     CDS             complement(12240..13229)
                     /gene="tdcB"
                     /locus_tag="ACHK3A_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO8741828.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13328..14266)
                     /gene="tdcA"
                     /locus_tag="ACHK3A_003802"
     CDS             complement(13328..14266)
                     /gene="tdcA"
                     /locus_tag="ACHK3A_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO8741829.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
BASE COUNT         3723 a         3847 c         3346 g         3450 t
ORIGIN      
        1 atatttatct taaatatcaa aacctttata acatccgaaa aagaaagata cgaatagcat
       61 ttacacaata cccggaatag actttcgtca cttttaatta aagggatgtt tttatgcagt
      121 ggtatttagc cgtattaaaa aattatgttg gtttctctgg tcgcgcacgt cgtaaagagt
      181 actggatgtt tactctgatt aacgccatcg tcggcgctat tatcaatgtc attcaattga
      241 ttttaggttt agagtttcca ttcctttctc tcatttacct ggcagcaaca atcattccgg
      301 tcattgctct gtgcgttcgt cgtctgcacg atactgaccg ttcaggtgcg tgggcgcttc
      361 tctatctggt tccgatcatt ggctggttag ttctgtttgt atttgcctgt ttagaaggca
      421 actctggcag caatcgttac ggaaacgatc cgaagtttgg ttcaaattaa ttatagaatt
      481 ggggcgatat ttcgcccctt tttattaaca ataacaaata ttattttccg ctaaaaagtt
      541 tgggaatttc ccgcagacac caggatttcg cctcccccat actgtcacga cgccaggcca
      601 taataatatc gatctcgctg gtcgattccg ggctgacgac acgcaaccgc ccttccgcga
      661 tgtctttttc gaccatcgga tacggcatcg tcgccacgcc aagccccgcc agtaacgcct
      721 gacgtttatc ttcaatcgtg ctcaccgtta aacgcggctg tttgtccagt agctgtacgg
      781 tcaataccgg gcgctcacga gcggtatccg ccaccgcaat tccacgatat ttcacgcgcg
      841 tcacttcaga taacggttcc ggctcctgat gaatcgggtg atcaggcgcg gcaacgtaga
      901 cgttcattaa cgtatagagc ttgcgcgagt tgatctccga cgaggagcga aaatgcatat
      961 ccggcgcgat aacaatatcc gcccgcccct gctccagccg ttcccatgcc cccgccagca
     1021 cttcggtgat gattgccagt tgggtattcg cttttgccgc cagtttgtca attaacggga
     1081 aaaaggcagg tgtcggcacc agcgcttcgg tcacaatggt gagatgcgtt tcccaaccgc
     1141 gcgcgagagc ttccgcatcg gtagtcagtt tatctgcggc ttccagcaaa acgcgtcctc
     1201 gctccagcaa catcctcccg acattggtga atttggtgcg atggcccgag cggtcaaaca
     1261 gcaccacatc cagctcttct tccagttttt gcatggtgta gctaagtgcg gaaggcacgc
     1321 gtcccagctc atccgccgcc gccgcaaaac tgccccggcg atcgatcgca tccataaccc
     1381 gtagtgcttc cagcgttaat gccctttctt tggccatttc gttctcattc aggaaatttg
     1441 aacataccgg gcagaatatc tggctaacaa tgtagcgtcc agccccttac cataaaagga
     1501 agtaaagaga ggtcaagaat tatgattact acccgaactg ccaggcagtg tggacaagca
     1561 gactacggat ggttgcaggc ccggtatact ttttcctttg gacactactt cgacccgaaa
     1621 ttgttaggct atgcctccct gcgcgtgctt aaccaggaag tgctggcccc aggtgccgcc
     1681 tttcagccgc gaacctatcc caaagtcgat attttaaatg tgattctgga tggagaagca
     1741 gagtatcgcg acagcgaagg caatcatgtt caggccagcg ctggtgaggc gttgctgctc
     1801 tctacccagc cgggtgtcag ctatagcgaa cacaatctca gcaaagacaa accgttaacg
     1861 cgaatgcagc tttggctgga tgcctgcccg cagcgagaga atccgctgat tcaaaaactg
     1921 gcgcttaata tgggcaagca gcaattaatc gcctcgccag agggcacagt gggtagcctg
     1981 caactgcgcc agcaagtgtg gctgcaccat atcgtgctcg acaaaggcga aagtgcgaat
     2041 ttccagttgc atggaccacg cgcgtatttg caatcgattc acgggaaatt tcatgcgctt
     2101 acgcatcatg aagagaaagc ggcgctaacc tgcggtgatg gggcgtttat tcgtgacgag
     2161 gctaacatta cgctggttgc cgattcccca ctgcgcgctt tgctgataga tttgcctgtc
     2221 tagttgtttt tacagggaga tgatgatgag taaaaaatcg gccaaaaagc gccagccagt
     2281 gaagcccgtg gtggcgaaag aacctgctcg taccgccaaa aattttggct atgaagagat
     2341 gttgagcgaa ctggaagcca tcgtcgcgga tgctgaaacg cgtttagccg aggatgaagc
     2401 taccgcgtaa agacaggtgc cggatgcggc gtaaacgcct tatccggcct acaaaaccca
     2461 acaacatcaa caaattgtgc aattcgtagg ccagataaga cgcgccagca tcgcatccgg
     2521 cacgataccc aaaactggcg tttatctggc cttgctcgcc ataatctcga taatctgccg
     2581 atccgtttgc tgcatcgaat ggcttgctaa cgcacacagg ttggcaatcg actgctcaac
     2641 atcatgcgcc acgatcccct cattgccggt cacggcggta tcatccagcg ccattaacac
     2701 cgctttccac gcagccgaag cactggtcga aaccttcatc gcgcagctgt tcgacgctcc
     2761 atcgcaaatc atgccgctga catcgccgat catactgctg atcgccatcg aaatgatttc
     2821 ataacgccca tccaccagcc atgccatccc ggcggcggcc cccattgctg cggtcgttgc
     2881 ggcacacagc gcagacaaac gcggtaactg gttatggatg taaattgcgc tcaaatgcga
     2941 aagcatcagc gcacgcgcca gccgttcatc atcggccccg aagtgttctg ctaccaccac
     3001 cacgggcatt gttgcagtga tcccctggtt acccgagccg gagttactca ttgccggaag
     3061 cgtagcgccg cccatacgcg catcggatgc cgcgctggta cgaatcacaa tggatgaaga
     3121 gagatctttc gccagcaaac cgcgtgcaca ctgtttttcc agcgtcgcgc caatatgcag
     3181 cccccactta ccgctcaaac cttcctgcga taacgcacaa tttagcttcg cggaatcgag
     3241 aataaagcgg atcgccgcaa acgggacttc attgacgaac ttcaggatct cagccagcgt
     3301 cgttcttgaa agcaccgtca gcggcgactc ttgctcgccc tctgtcacac acgcctgctg
     3361 ggtaaacacc acaccattgt gcgtctcaat atgcacaatg ttggtatgcc cgccgacgat
     3421 ggtgacacac gcccacttct caccgttcca gactttggcg cgtgagaaga ggatttcatc
     3481 gcaaggttcc tggatcttaa ctgagacttt ccccgccgcc agcagtgctt tggcatcggc
     3541 aattgcctgc gcagttgcgt ctttcagcac ttccagcccg gcgttagcat ttccacctaa
     3601 cgctcccagc gccgccgcaa ttggcagccc caccattccc gtgccgggaa cggtgacgcc
     3661 cagaccgttc ttcatcagat ttggcgaaac ccaggcttct acacgttcaa ccggaccttc
     3721 cagttctgcc gcagcaaccg ccgccgccag cgccagtgaa atcggttcag tacatcccag
     3781 cgccggtttt acttcctcct gaacggcgag gatgtaacgc tgccataacg gatttaaagt
     3841 cgaatcaaac atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg ccaggaacgg
     3901 agaaacacaa agcaacaaac cagtgacgat aatcaggtac agagacatcc ctttgtattt
     3961 gtgcaatgcc ggtactttgt aaaccagcca cgccgggatc aggcacccta ccatgccgaa
     4021 aatcgggcta cagatagagg tgaagctcaa caccggtgcg ttcagtacga tggcgctcca
     4081 ggccagcaaa atggcgaaaa tcatgatgcc gcgctgaacg agattttcgt taatcttctc
     4141 ggcaggcatc ttgcgacgca ggatgttcat tacgatccct tgcgttgctt cgcgaaagcc
     4201 taaatagacg ccaaagaatg cagtcattac agcaaagata ttgagaatga cgctgaccac
     4261 tttcacccac gctgcgccat caccgctaat aaactgtgcg gcaatcgcca acgcggaaat
     4321 attctgctcg taggctttta ccgcttcgtc atgtcccatc gccagcgtga acgacacggc
     4381 gtagaaaaag acggtgacaa acaaaatgcc aaacgcgata ttcattgccc gcaatgcttt
     4441 atgccgcgcc acttcaattg atttttcccg cgagcgataa gagatcacca tcgggcttaa
     4501 cgtctggata aacagaatcg acgtcagggt aaacggcagc gtaataatgg cgtttttcac
     4561 cagcagcccc agcggcggta gcgaaccgac gttatacaga tgccacattc ccaccatcga
     4621 cacgcccagc gccgcgacca ccagcagctt ggtcagcacc atgccggtcg aaattttaaa
     4681 taacaatttc tcgccgcgtg aggagatcgc caccagaatg caaatcagta ccagaccata
     4741 aaagggactg tctgacagca ccccttccgt cacgccgaag gtatgcaggt aggaagcact
     4801 atcgttggtg atggcggtgg aatagacgaa catccaaatc accagcatta cgaaatagag
     4861 cgcacctaac aggatgcccc agtttttacc taaataaccg ctgatgacgc tcgggtaatc
     4921 tttacactcc ggtgattctg ccagcgtatt aataaacaac cgctgaaaca gatacattgc
     4981 cgggtaacca atcaccgatg agagcaaaaa tacccacaat cccatcaaac cgacctgcac
     5041 cgggagaaaa acaatccccg cgccaatcgc catcccgata ctcataatca cccagccggt
     5101 gtcagtactg tcgaatttga tcgcttctcg ccactcgctc tcactcattc cagcacgacc
     5161 cgccggggtc gaagcgtctg caatgacgcc tttattcgat gcaatttcca taatttctcg
     5221 ctcaatattt tgtagggctt attatttttt tccgagccgc atcaaggcga tatgcggtct
     5281 acgtttttgc aggcgagtta ttagaataaa gaaatgatac gcgcgaggtc agagaaaatc
     5341 ttcacaatca agactttgaa ataacggaga tggataagaa ttttctacta aattaatcgc
     5401 aagagaatat tcattctcta tttgcggcag atcacaaaaa aaaggtgcac atttgtgcac
     5461 ccaaggatga aagctgacag caatgtcagc cacagaccac tttaatggcc agtcctccgc
     5521 gtgacgtttc gcggtattta tcgttcatat ctttgccggt ttcatacatc gtctcgatca
     5581 ctttatcgag tgaaacacgc ggtgccgagg tgcggcgcat cgccatccgc gcggcgttta
     5641 ctgctttcac ggcattaatg gcattacgtt caatgcacgg gatttgtacc tgtccggcaa
     5701 ccggatcgca ggtcagccca aggttatgct ccatcgcgat ttccgccgca ttgcatacct
     5761 gcgccggact gccgcccagg agttcagtta accctgccgc cgccattgaa caggccacgc
     5821 caatctcccc ctgacagccg acctccgcac cagagatgga ggcgttcatt ttatacagcg
     5881 cgccaatagc ccccgctgca agaaaatagc gggcaattga ccgctcgttt accggacgac
     5941 ggaacttatc gtaataagcc agcactgccg gaataatgcc gcacgcaccg ttagtcggtg
     6001 ccgttaccac gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg tacatattga
     6061 tccagtcgat gacattcatc ggatcgttag agatgttatc gctggaaacc agttgacgac
     6121 gcagcgctac ggcacgacgc ggcacattga gcggaccagg cagcacgcct tcagtgttca
     6181 tcccgcgttc aataccgtca tgcatcactt gccagatacg ggcaaaccct gcgtcaattt
     6241 ccgctttact gcgcagcgcc agctcgttgt gcatcatcag gccagatata gacaggccgt
     6301 tgtaatcaca cattttcagc agttcacctg ctgagtggaa atcgtaaggt acgggtgttt
     6361 cgacatcgtg cgacaggccg aagtgttctt cttcgacaat aaacccgccg ccgacagagt
     6421 aataggtttt gcttaatagc tcttcctgtc ccttccaggc agtgatccgc attccgttct
     6481 catggcgagg caacatttcg ggatggaaga taatgttctt tgctacagga aaatcaacaa
     6541 tatgcgcgcc agaagccacc ggcaaccgcc cgctgcgcgt cactaactct ataaatgcag
     6601 ggatctcatc aatgacaaca tcctgcggac tgtttcctgc cagtcccatg atgatggcga
     6661 catccgtggc atggcctttg cccgtcagtg acaacgaccc gtacagatcg accacaatat
     6721 ggctcgtcgc ggttaataag ccgctacttt ccagccgatc aataaaactt tttccggcat
     6781 tcattggccc cacagtatgc gaactggagg gaccaatccc aattttgaaa atatcgaatg
     6841 cactaatcat atccacaccc tcggattgcc gttcagtgaa gtggagcgga acgaccttgc
     6901 gaccgtcccg ctcacgaggc cttacgcact acgtactgcg atggcttcaa tttccaactt
     6961 cacatctttc ggcaaacgcg cgacctgcac acagctccgg gtcgggtagg tcgcctgatg
     7021 ttcatcgaag aactgcttat agacttcatt gatggtggca aaatcattca gatcggtgat
     7081 gaacacggtc atcttgatga tatcgcccac actcagcccg gcagcaacca cgatcgcttt
     7141 gacgttttcg aggcttaaac gcgcctgatc ttgcacatca gccgggatct caccggtctg
     7201 tgggcaaacc ggaatttgcc cggaggtgaa gaccatgctg cctaaatcaa cgccctgaac
     7261 ataagggccg attgcgcctg gggcacgttg cgtttcgata atctttttca tacatcctcc
     7321 ggcgtcagag cgcctgggta aaggtacgtg aaataacatc ctgttgctgt tcacgggtca
     7381 gtgcgttgaa gcgcacggcg tagccagaga cacggattgt caggttagga tatttttccg
     7441 ggtgctcgat ggcatccagc agcatttccc gattcattac gttgacgttg aggtgttgac
     7501 cgccttcgac atccgcttcg tggtggaaat acccatccag caggccgaca aggttggttt
     7561 tacgtactgg atcttctttg cccagcgccg cagggacgat ggagaaggtg tacgagatcc
     7621 catctttggc gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc acggcgcctt
     7681 tgcgatcacg accgtgcatc gggttagcgc ccggcgcgaa cggtgttccg gcgcggcgac
     7741 cgtccggcgt gttcccggtt ttctgaccgt acaccacgtt agaggtgata gtcagaatcg
     7801 attgagtcgg cacagcattg cgataggttg gcagagcttt aattttcttc ataaagcgtt
     7861 caaccagatc gcaggcaatg ctgtctacgc gctcgtcgtt gttgccatac tgcggatatt
     7921 cgccgtcgat ttcgaaatcc accgccaggc cgttttcgtc acggattggt ttcacacgag
     7981 catatttgat ggcagacagg gagtccgttg ccaccgacag gcccgcgatg ccgcacgcca
     8041 tggtgcgata gacatcacga tcgtgtagcg ccatcagcga ggcttcatag ctgtacttgt
     8101 cgtgcatgta gtggatgata ttcagcgcgc tgatgtactg taccgccagc cagtccatga
     8161 agtggtcgag gctatccatc actttgtcgt agtccagcac gtcatccatc aacggagccg
     8221 ttttcgggcc aacctgaatc ttcagcttct cgtccattcc gccgttgatt gcgtacagta
     8281 gcgttttcgc caggttagcg cgcgcaccga agaactgcat ctgcttacca atcaccattg
     8341 ggctgacgca acaggcaatc gcgtaatcgt cgctattgaa gtcagtacgc atcagatcgt
     8401 cgttttcata ctgcaaagag gatgtgacga tagacacctg cgcggcatat tttttgaagg
     8461 caatcggcaa ggcttccgac cacagaatag tcaggttagg ttccggtgca ggccccatag
     8521 tgtgcagggt gtgtaaatag cggaaggagt ttttggtcac cagggttcga ccgtccagcc
     8581 ccatcccgcc gatcacttcc gtcgcccaga tcgggtcgcc ggagaacagc gaatcaaatt
     8641 ccggcgtacg caagaagcgc accatacgga tcttcatgat gaagtggtcg atcagttcct
     8701 gtgcctgctg ctcattgagt acgcctgctt taaagtcgcg ctcaatgtag atatcgagga
     8761 acgatgccgt gcgccccagc gacatcgcgc cgccattttg cgatttcact gccgccaggt
     8821 aagcgaagta gagccactgc accgcttcct gtgcattctg cgccggacgg gagatgtcaa
     8881 aaccgtattt cgccgccatt tcttgaatct gcaacagcgc acgacgatgt tccgccagct
     8941 cctcacgcag acggatcgta gcttcgagat cttcgccttt ttccagacgg gactggagat
     9001 cggcaaactg cagttcgcgt tcacgtacca gataactgat gccgtaaagc gccacgcggc
     9061 ggtagtcacc gatgatacgc ccacgaccat aaccatctgg taaaccggtc agtacaccag
     9121 atttacggca gcgcagcata tccggtgagt aaacatcaaa tacgccctgg ttatgggttt
     9181 tacgcagatc ggtaaagatg tattcaaatt cgctatccat ttcacgacca tacgcgtgga
     9241 atgaacttct aatcatatta atgccgccga acggatgcag cgcacgcttt aatggcgcat
     9301 ccgtttgcag acccactatt ttttccagcg gctggtcaat atatcctgca tcatgagcgg
     9361 taattgtggt ggcaatattg gtatcgaaat ccaatggcgc gtgagtcgca ttttcgatac
     9421 ggatgccttc catcactttt tcccacaatt ccgtggtggc aggcgtcgct tcggcgagaa
     9481 aagattcatc accttcatac ggtgtatagt tatgttgaat aaaatcgcgg acattaattt
     9541 cgtttttcca gtccgtacct ttaaagccaa gccatgcgtc ggcgtacagc ttatcgctgg
     9601 tatcaatatc taccttcatg aaaaataatc tctctacaat acttcaacta aattatgcaa
     9661 attctgcggg cgcgttcact ttgcctaaat gaatggcatc caaagcaatc attttttctt
     9721 cgttagtcgg aattacggcg caaatgacac gcgcattttc actggaaaca attcgctctc
     9781 caaaagagtt agagcgatta ttcatttctg tatcaatctc tacgcctaat acagctaaat
     9841 gttccatgac cagacgacga attaaacttg aattctctcc tattccgccg gtgaatataa
     9901 ttccatccag gcgatgtaat gaagcagcgt gtccggcaat atgacgggca attcgatgaa
     9961 caaaggtttt aattgccaat tgcgcgcgtt cgtgaccttc atgccaggct ttttccagaa
    10021 cacgtaaatc cgaagaaagg ccggaaatac ctaataatcc cgactcttta ttcacgacgc
    10081 gttccaggtc acccaggctc tggttggttt ggctggcgac ccaggacatc gccccgaagt
    10141 cgacatcacc actgcgggta cccatcatca agccttccag cggcgtcatt cccattgagg
    10201 tatcaacact ctgaccgttg cgaactgcgc agattgacgc gccattgcca agatgcgcca
    10261 caaccaggcc ggagtcatct tccgccagat tcagcaaaga atgcgcgcgc tgggaaacat
    10321 agcggtgcga cgtgccgtgg aaaccataac ggcgtacacc taactcttca taatatttcc
    10381 acggcaggcc gtataaataa gcttccggag ccatcgtctg gtggaaactg gtatcaaata
    10441 ccgctacctg agttacgccc ggaaataatt gctgcgccga ttcaatacca cttaaattgg
    10501 cgtaattatg caggggtgcc agtggagaaa cgcgacggat attatcaatg acttcatcgg
    10561 taataatggc ggactcggta aaaatactgc cgccgtgagc gatgcggtgg ccaattaagg
    10621 ccacactgtc atttaaattc cgtttttcca gttcaaatgc aattgccttc aatgcacctt
    10681 cgtagctgtg gtgagccagc ggtgctggct ctcccccatt tacggataag aatgcatttt
    10741 ccgagttaat accgtcggca atacctgaca ttaatacttc acagtcgctg gcattgagta
    10801 cggaaaactt aatcgaagac gaaccacagt taataaccaa aacaaccgga aattcattca
    10861 tctcttttct catcctgagt tacggattaa aacagtttgt atacgatgtt caggatagtc
    10921 agcagaccaa tcacggtaac aaacacgtta tccagacgac cacggtattt cgccagagac
    10981 ggcgctttac gaatggcata catcggcaac aggcatagca gtgatgcgat aatcggtgcg
    11041 cccatggctt caatcaggtc gaggatgttc gggttggcgt aggcaacaac ccaggtggag
    11101 cccatgatga agatcatgct gagagtattc agtttaccca gcgacacttt ggttttgtca
    11161 cctttataac cgaacttcag aatcaggcca ttcaagcctt ccagcgtccc cagatagtga
    11221 ccgaagaaag atttgaagat agccacgagt gcgatgatgg aagccgcata ttccagtgta
    11281 atcgcgaacg ttgttttggt accggtcatg gacgcaaagt ggttagccag ataagaaagc
    11341 actggaatat tctgcgcttt ggcttccgcc atgttggccg gagacagcgt aaacaggcag
    11401 ctaaaggcaa agaacatcac cactgcaacc atcagcatgc tggcacgaga aatgatttgg
    11461 gaacatttac gttcggtgaa gtcgcgaccg aagtctttct catactcttc acgtttagaa
    11521 accacgaagg aagagacgat tggcgagaag ttaaaggaga aaaccatgat ggaaatcccc
    11581 agccagacag tgatcaggat accgtcatga ccggttaacg acagcgaacc gaggtcaacc
    11641 tggtcgataa ctgcagagtt ccagtaaggg atcagcgaca gagaaatcag caccaggctg
    11701 gcgataaacg gccataccag gtagctcatc actttaacca tcagatcctt accaaaccag
    11761 atgacgaaag ccatcagcag caacaggaac agcgccacaa agccgcgatt cagcggtgca
    11821 aagccgagct ggttttccca gaacgtcata aaggtattgg taatagtaac gccataaatc
    11881 cacagcagtg ggcaaatcgc gaagaagtac aggaacgtga taaccacgcc gccagtttta
    11941 ccaaaatgct cttccaccgt ttccgtaatg ttgccggaag ggttagagcc agaaagacac
    12001 agacgcgcca gtgcccggtg gcaataaaac gcgatggggt atgctaatac caacatcaga
    12061 agaatcggga tcagtccgcc aaaacctgcg cggataggga agaacagcac cccggcgccg
    12121 attgccgtac caaacaagcc taacgtccat gtggtatctg atttacgcca ggacgattgt
    12181 tttgtctggc tggatacaat gctatctgaa gtactcatat cctatcctca acgaattaat
    12241 taagcgtcaa cgaaaccggt gatttgagag acgcgagaaa gatcgatatt gccgccggaa
    12301 ataatactga cggtttttct gttttgaata tattggtcta atttaccgct taataatgca
    12361 gcacatgcca gagcgcctgc gccttcggtg acgactttat tgcgctgaat taaggcaatc
    12421 atactgtttc tgatttcgtc ttcgctgacc agcacgatgt catcgactaa ttcacgaacg
    12481 atttcgtaag ttaaattacc cgggcgggag acatcacaac catccgccag ggtgccggta
    12541 gttcggtgcg tagttatttc tccggagtgg aaagaagccg ccatgccgtg aacgttttct
    12601 gactgtacgc caataacacg aatagtcggg ttaattgatt taatcgctac cgcaatacca
    12661 gcaattaaac cgccaccgcc aattggcaca atcacattat cgacatcata gagatcttcc
    12721 ataatttcca gaccaatcgt tccctggcca gcaatcactt tcggatcatc gtaaggtggg
    12781 ataaaaatac ggccttccat ttcgacaatt tcgctcactt tagcaatagt gtcgttgaag
    12841 ttatcaccat gcaaaacgac ttctgcggag tagtcgcacg ttgccgcgac tttggatttc
    12901 ggcgcacctt ttggcatcac cactttaccg tcaataccca gcatcgcaca ggagagcgaa
    12961 accccttgcg catggttgcc cgcagagcag gccactacac ctttacgttt ttccgcatca
    13021 gtcagtgaac ttaatttatt aaatgcgcca cgaattttaa atgaaccggt acgctgcatg
    13081 ttttcgaatt tgaggaatat ttcacctttg caacgttcac taaaatagtt ggagcgaggc
    13141 atacctgttt tataaattcg cccagccagt cgttgttttg cttcaatgat gtcatcaata
    13201 gcaaccggga gatcgtatgt aatatgcatt ataaaacctc ttagcctgaa ttaaatatgt
    13261 aggtaaccgt aaccgacacc tgcaaaacag acaggtggat tatttatgtt taaattaaaa
    13321 caaataacta atcaacttca attaattgcc ttcgtctaca cccattataa gatgaatact
    13381 ctttggctaa ttccaccaaa accgatgctg cttttttaat acgataattt ttcgaccata
    13441 ccgcggcata ttgtgccaca ggtaatgttt cttcaaccgg aatagtaata aattgattag
    13501 aaccaaaagg tgatgtcata tcacaaggaa ttacagttaa gaaatcagca ttgagaacaa
    13561 gattataaat tgtcacgact gagtcggttt taacgatgtt ttcaatactg atgccatttc
    13621 tttgtaacgt agtaagtagt tcgctgtagt accccatatt ggtttgtggc aacacccact
    13681 gttcgttctt caacgactcc agcgtggtgg tgccggtgca tgttcgggac ttactggcta
    13741 ccagcacaaa ctcggactcg aacagcggct caacatgtaa atcctgaagc ttcatttctg
    13801 cgcttaacgt accaatcgca aaatccagtc gaccgtcgcg gattgccggt aagaacgaag
    13861 acagttgcgc ttcatacata gaaacctgcg ctttcgggaa cacctctttg aacttgttga
    13921 tcatccctga cataaaagta aaaccaatca atgaaggaaa accaaatgag acttccacca
    13981 ccgcctcaga agacataccg cttatctcat taaccatatt tttcatttca cgggtaatgg
    14041 attcggaacg ggagagtaac aattgaccgg caggtgttaa tgttacaccg gtatttttcc
    14101 gcaccactaa ctccacacca aaataatctt caatatcgtt aatgatttta ctgacggccg
    14161 gttgagttaa ccctaactct tttgcagccg agccgataga accacttcta ataacttcct
    14221 gaaagactac caggtgctgc gttttcggaa gaagaatagt gctcataacg acctacgtta
    14281 attacctcat tgacggcatg aagtgtatca aaatgaaatg aacaggatat gtgcgaccac
    14341 tcacaaatta actttcaata ctttcc
//
LOCUS       DAZGHF010000120        14267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGHF010000120 DAZGHF010000000
VERSION     DAZGHF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.121"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            18..833
                     /gene="gspC"
                     /locus_tag="ACHK3A_003803"
     CDS             18..833
                     /gene="gspC"
                     /locus_tag="ACHK3A_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO8741830.1"
                     /translation="MPTLRFPFHLANHNKDAAINILIIFISIGLIIFNVNYFHTTIVK
                     NGQIINQPTNAFQSEFSLAALWRNENHAGVKDANPVAVNQETPKLSIALNGIVLTSND
                     ETSFVLINEGSEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDI
                     IKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRHERTQ"
     gene            817..2769
                     /gene="gspD"
                     /locus_tag="ACHK3A_003804"
     CDS             817..2769
                     /gene="gspD"
                     /locus_tag="ACHK3A_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO8741831.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNERKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHTPAPSAR"
     gene            2779..4260
                     /gene="gspE"
                     /locus_tag="ACHK3A_003805"
     CDS             2779..4260
                     /gene="gspE"
                     /locus_tag="ACHK3A_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO8741832.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLENEA"
     gene            4257..5453
                     /gene="gspF"
                     /locus_tag="ACHK3A_003806"
     CDS             4257..5453
                     /gene="gspF"
                     /locus_tag="ACHK3A_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO8741833.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTAVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQHTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLSNLEIRKRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5463..5900
                     /gene="gspG"
                     /locus_tag="ACHK3A_003807"
     CDS             5463..5900
                     /gene="gspG"
                     /locus_tag="ACHK3A_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO8741834.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5908..6417
                     /gene="gspH"
                     /locus_tag="ACHK3A_003808"
     CDS             5908..6417
                     /gene="gspH"
                     /locus_tag="ACHK3A_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO8741835.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6414..6791
                     /gene="gspI"
                     /locus_tag="ACHK3A_003809"
     CDS             6414..6791
                     /gene="gspI"
                     /locus_tag="ACHK3A_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO8741836.1"
                     /translation="MNKQSGMTLLEVLLAMSIFATVALALMSSMQGQRNAIERMRNET
                     LALWIADNQLKSQDTFNDENTSSSGKEIINGEEWNWRSDVHSSKDGTLLERTITVTLP
                     NGQKTALTRYQSIHDRSGQAQDD"
     gene            6784..7371
                     /gene="gspJ"
                     /locus_tag="ACHK3A_003810"
     CDS             6784..7371
                     /gene="gspJ"
                     /locus_tag="ACHK3A_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO8741837.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLGVLAFMVFSQASTLHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPQAPLNEAQTL
                     QTVHWYLRNHTLYRAVRTSVDGRKNQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7364..8347
                     /gene="gspK"
                     /locus_tag="ACHK3A_003811"
     CDS             7364..8347
                     /gene="gspK"
                     /locus_tag="ACHK3A_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO8741838.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQFWPQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8362..9525
                     /gene="gspL"
                     /locus_tag="ACHK3A_003812"
     CDS             8362..9525
                     /gene="gspL"
                     /locus_tag="ACHK3A_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO8741839.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTPTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMRCYGVAPHGVAATNPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QHQQQETWQRYFPQIKRTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMQKTEKDPVSGVWTVRSSGK"
     gene            9522..9983
                     /gene="gspM"
                     /locus_tag="ACHK3A_003813"
     CDS             9522..9983
                     /gene="gspM"
                     /locus_tag="ACHK3A_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO8741840.1"
                     /translation="MIKSWWAEKSTSEKQLVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEQITLKHLSFREQP"
     gene            9983..10660
                     /gene="gspO"
                     /locus_tag="ACHK3A_003814"
     CDS             9983..10660
                     /gene="gspO"
                     /locus_tag="ACHK3A_003814"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFO8741841.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10689..11165)
                     /gene="bfr"
                     /locus_tag="ACHK3A_003815"
     CDS             complement(10689..11165)
                     /gene="bfr"
                     /locus_tag="ACHK3A_003815"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO8741842.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREES"
     gene            complement(11237..11431)
                     /gene="bfd"
                     /locus_tag="ACHK3A_003816"
     CDS             complement(11237..11431)
                     /gene="bfd"
                     /locus_tag="ACHK3A_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO8741843.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(11600..14266)
                     /gene="chiA"
                     /locus_tag="ACHK3A_003817"
     CDS             complement(11600..14266)
                     /gene="chiA"
                     /locus_tag="ACHK3A_003817"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFO8741844.1"
                     /translation="MGLVCSALPALAMEVWNNQQGGNKYQVIFDGKIYENAWWVSSTN
                     CPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGS
                     ATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNP
                     WRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNYAVV
                     AWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEISQY
                     GNPGSCSVKPDNNSGAVTPVDPTPETPVTPVTPTPDNNEPSTPADSSNDYSLQAWSGQ
                     EGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSAENSNNPWRLVRTATAAEMSQYGNPTT
                     CEIDNGGVIVADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPA
                     KAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKAQSY
                     SNEELNNAPQFNPETLYASDTLIRFNGANYISQSKVQKVSPSDSNPWRVFVDWTGTKE
                     RVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWG
                     TAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLN
                     LRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGM
                     NVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHSQLKGLH
                     PNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLP
                     GGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT         3666 a         3677 c         3399 g         3525 t
ORIGIN      
        1 ttattagaac atacatcgtg cccacactac gcttcccctt ccatctggcg aatcataata
       61 aagatgctgc catcaatata ttgattatat ttataagcat agggttgatt atttttaatg
      121 tgaattattt ccatacaact atagttaaaa atggacagat cattaatcaa ccaacaaacg
      181 catttcagtc tgagttcagc cttgccgctt tatggcgtaa cgaaaatcat gctggggtaa
      241 aagatgcaaa tcccgtggca gttaatcagg aaacacctaa gctctctata gcgcttaatg
      301 gcattgtact cacatcgaat gatgagactt cttttgtctt aattaatgag gggagcgaac
      361 aaaagcgata ctccctcaac gaagcccttg agtcagcgcc gggaacgttt attagaaaaa
      421 taaataaaac cagcgttgtg tttgaaactc acgggcatta cgaaaaagta actctccatc
      481 cagggctacc tgacatcatc aagcagcctg attcagaaag tcaaaatgta ctcgctgatt
      541 atattatcgc cacgccgata cgtgatggag aacagatata cgggcttcga ttgaaccctc
      601 gtaagggcct caatgcattt accaccagct tgctacaacc aggggatatc gccctcagga
      661 tcaataatct ttcgctgacc caccctgatg aggtttcaca ggcattaagt ttactgctaa
      721 ctcaacaaag tgctcagttt acaattcgtc gcaacggcgt accccgcttg ataaatgttt
      781 ccgtcgcgga acttacagga atgaatggac agcgtcatga aaggactcaa taaaatcacc
      841 tgctgcttgc tggcagcact actcatgcct tgtgcagggc acgctgagaa cgaacaatac
      901 ggcgctaact tcaataacgc cgatatccgc cagttcgtgg aaatagtggg tcagcatctt
      961 ggcaaaacga tcctgatcga cccttcggta cagggaacca tttccgtacg cagtaatgat
     1021 acgtttagcc aacaggagta ctaccagttc tttttaagta ttcttgatct ttacggttat
     1081 tccgtgatca cgctggacaa tggttttctg aaagtggtgc gctcagctaa tgtaaaaaca
     1141 tcgccaggga tgattgctga cagttctcgt ccaggcgtag gtgatgagtt ggtcacccga
     1201 atcgtaccgc ttgagaacgt tcctgctcgt gacctggctc ccctgctccg ccagatgatg
     1261 gatgcgggta gcgtcggtaa tgttgtgcat tatgaaccct ccaacgttct tattctgacc
     1321 ggtcgtgcct ccaccattaa taaactgatt gaagtcataa agcgcgttga tgtcatcggc
     1381 acagagaagc agcaaattat tcatctggaa tatgcgtcag cggaagatct cgccgaaatt
     1441 ttgaatcaat taatcagcga aagccacggt aaaagccaga tgcctgccct cctttccgcg
     1501 aagattgtgg cggataagcg aaccaactct cttatcatca gcgggccgga aaaagcacgc
     1561 cagcgcatca cttcattact gaaaagcctt gatgtagaag agagcgagga aggaaatact
     1621 cgggtttatt acctgaaata tgctaaagcc acgaatctgg tggaagtgct aaccggtgtt
     1681 tccgaaaagc tgaaagatga aaaagggaat gagcgtaagc cctcctcttc tggcgcgatg
     1741 gataacgtcg ccattaccgc cgatgaacag actaactctc tggtcattac cgctgaccag
     1801 tccgtccagg aaaaactcgc cacggtaatt gcgcgtctgg acattcgccg tgcacaggtg
     1861 ctggttgagg caatcatcgt tgaagttcag gatggaaatg gacttaacct cggcgtgcaa
     1921 tgggcgaata aaaacgttgg agcacagcaa tttaccaata ccggattacc gatttttaac
     1981 gctgcgcaag gtgtggctga ttataaaaag aatggtggga tcaccagcgc gaatcctgcc
     2041 tgggatatgt ttagcgccta caatggcatg gccgcaggct ttttcaatgg cgactggggc
     2101 gtattgttga ctgcgctggc cagtaacaat aaaaatgaca tcctcgccac cccgagcatc
     2161 gtaacgctgg ataataaact cgcgtccttc aacgttggtc aggatgtgcc ggtgctatct
     2221 ggctctcaga ccacttctgg ggataacgtc tttaatactg tcgaacgcaa aacggtgggc
     2281 acaaaactca aagtcactcc gcaggtcaat gaaggcgacg cggtactgct cgaaatagag
     2341 caggaggttt ccagcgttga ctcttcgtct aattcgacgc tcggcccgac atttaacacc
     2401 cgaactattc aaaatgccgt gctggtcaaa accggtgaaa cggtggtcct gggcggattg
     2461 ctggatgatt tttctaaaga gcaagtgtca aaggttcctc tgcttggcga tattccttta
     2521 gtggggcaac tcttccgcta tacttcgacc gagcgcgcta aacgcaacct gatggtattt
     2581 atccgcccaa cgattatccg tgacgatgat gtttatcgct cactgtcaaa agagaaatac
     2641 acccgctacc gtcaggagca acaacagcga atcgacggga aatcaaaagc gctggttggc
     2701 tcggaagatt tgccggtgct ggatgagaac acgttcaaca gccatacccc tgcgccctcg
     2761 gcacggtaag gcattcttat gagaattcac tcaccgtacc ccgccagttg ggcgctggca
     2821 caacgaattg gttatctcta ttcagagggc gagattattt atctcgccga tacgccattc
     2881 gaacggttac tcgatattca acgtcaggtt ggccagtgcc agaccatgac cagcttgtca
     2941 caggctgatt ttgaagcccg gctggaagcg gtgttccatc agaataccgg tgagtcgcaa
     3001 cagattgcgc aggatatcga tcaatccgtc gatcttctct cgctttcgga agagatgccc
     3061 gcaaatgaag atctcctgaa tgaagattca gcggcaccgg ttatccgctt gatcaatgcg
     3121 attttgagtg aggccatcaa agaaaccgcc tccgatatcc acattgaaac ctatgaaaaa
     3181 acaatgtcga tccgttttcg catcgacggt gttttgcgga caattttaca gccaaacaaa
     3241 aaactggcgg cactgcttat ctcccgaatt aaggtcatgg ctcgtcttga tatcgccgaa
     3301 aaacgtattc cacaggatgg acgtattagt ttgcgtatcg ggcgacgtaa catagatgtc
     3361 cgcgtatcca cactgccgtc catctatggt gaacgcgccg tgctccgtct gctggataaa
     3421 aacagcctcc agctttcatt gaacaacctg gggatgacgg cagcggataa acaggattta
     3481 gaaaatctca tccagcttcc acacggtatt atcctggtga ccggaccgac aggctccggt
     3541 aaaagcacca cgctctacgc catcctttcg gcgctgaata ctcccggccg caatattctg
     3601 acggtagaag atcccgtgga atatgagctg gaaggcattg ggcaaacgca ggtgaatacc
     3661 cgtgtggata tgtctttcgc tcgcggcctg cgcgccatac ttcgccagga cccggatgtc
     3721 gtcatggtgg gggaaattcg tgatacagaa accgcgcaga ttgcggttca ggcctcgctc
     3781 accggccatc tggtactctc aacactccac actaacagtg catcaggcgc agtgacccgg
     3841 ctccgcgaca tgggcgtcga atcattcctg ctttcgtctt ccctggcagg gattatcgcg
     3901 caacgtctgg ttcgtcgcct gtgtccgcaa tgccgacaat tcacgcccgt atcaccccaa
     3961 caagcgcaga tgtttaaata tcatcagctc gcggtgacaa caattggcac tcccgtaggc
     4021 tgccctcatt gccatcaatc cggctatcag gggcgcatgg cgatccacga aatgatggtg
     4081 gtgacgccgg aattacgggc cgctattcat gaaaatgtgg atgaacaagc actggagcga
     4141 ctggtccggc aacaacacaa tgccttaatc aaaaatggcc tgcaaaaagt gataagcggt
     4201 gacacctcct gggatgaggt tatgcgcgtc gccagtgcca cgctggagaa cgaagcatga
     4261 attatcgcta tcgcgccatg acccaggatg gtcaaaaatt gcaggggatc attgatgcta
     4321 acgatgaacg tcaggcacga ctgcggctgc gtgaagaagg gcttttcctg ctggatattc
     4381 gcccccaaaa aagttcggga gtaaaaacac gtcgcccgag gatcagccat agtgaactga
     4441 cgcttttcac ccggcagttg gcaaccttaa gcgcagcggc attacctctg gaagagagcc
     4501 ttgccgtaat cggtcaacaa agcagtaata aacgactggc tgatgtgtta aatcaggtac
     4561 gcagcgccat ccttgaaggg catccccttt ccgatgcgtt acaacatttt cccacgcttt
     4621 tcgattcgct ctaccgtacc ctggttaaag cgggcgaaaa gagcgggctg ctggccccgg
     4681 tgttggaaaa actggctgat tacaatgaaa atcggcagaa aatccgcagc aaactcattc
     4741 agtcgctgat ctacccctgc atgctgactg cggtggcgat tgtggtcgtg attattctcc
     4801 tcactgctgt cgtgcccaaa attaccgaac agttcgtgca tatgaagcag caactgccgc
     4861 tgagtacacg cattctttta ggtctgagcg acacgttgca acataccggc ccgacattac
     4921 tagcgacagt gtttattgtc gctgtaggtt tctggctctg gttaaaacgc ggcaataacc
     4981 gccaccgttt tcatgccatg ttgctgcgcg ttgcactcat cggcccgctg atttgcgcca
     5041 ttaacagcgc acgctatctc cgcactttaa gtattttgca atccagcggc gtccctctgc
     5101 tggatgggat gaatttgtcc accgaaagtc tcagcaacct cgaaatccgc aagcgtctgg
     5161 caaatgcggc agaaaacgtt cggcagggta acagcattca tctttcgctg gaacaaacgg
     5221 caatcttccc gccgatgatg ctctacatgg tggcctctgg cgaaaaaagc gggcagctcg
     5281 gcacgttaat ggtcagagca gcagataacc aggagacact ccaacaaaat cggatcgcct
     5341 taacgctctc catcttcgag ccagcactca ttattacgat ggcactgatc gtcctgttta
     5401 ttgtcgtctc ggtactccaa cctcttcttc aacttaactc aatgattaat taaggaaagt
     5461 atatgcgcgc aacggataag caacgcggtt ttacattact ggaaattatg gtggtgatcg
     5521 tcatcattgg cgtacttgcc agcctggtgg ttcccaacct gatgggcaat aaagaaaagg
     5581 cggataaaca aaaagccgtc agcgatattg tggcgctgga aaacgccctg gatatgtaca
     5641 agctcgacaa tcatcgctat ccaaccacaa atcagggact tgaatcctta gtcgaagcgc
     5701 cgacactgcc accgctggcc gcaaactata acaaggaagg ttatatcaag cgtctgcctg
     5761 ccgatccctg gggcaatgat tatgtcctcg ttaatcctgg tgaacatggt gcatacgatc
     5821 tgctttcagc agggcccgat ggtgaaatgg gaaccgagga cgacatcacc aactggggtt
     5881 tgagcaagaa gaaaaagtaa gtcagcgatg aatcagcaac gcgggtttac cctgctggag
     5941 atgatgctgg tgctggcgct ggtcgctatc acggcaagtg tggtgctctt cacatatggt
     6001 cgggaggatg ccgccaacac tcgtgcgcgc gaaacagcgg cgcggtttac cgctgcactt
     6061 gaattagcca tcgaccgcgc tacgctcagt ggtcagcctg tcggtatcca tttctctgat
     6121 tcagcatggc gcattatggt accgggcaaa acaccatcag cctggcgctg ggttccatta
     6181 caagaagatg ccgcagacga gagcaaaaat gactgggatg aagaactctc aattcatctt
     6241 cagccattta aacctgatga cagcaaccaa ccgcaggtgg tgattctggc cgacggacaa
     6301 atcacgccct tctctttgtt gatggctaac gcagggaccg gtgagccgct cctgacgtta
     6361 gtctgttcag gatcatggcc cctggatcaa acgctcgcca gagatacccg cccatgaaca
     6421 aacaatcagg gatgacactg cttgaagtct tactggcgat gagtattttc gccactgttg
     6481 cgctggcttt aatgagcagc atgcaagggc aacgaaatgc catcgagcgg atgcgtaatg
     6541 agacgctggc gctgtggatc gcagataacc agcttaagtc gcaagataca ttcaacgatg
     6601 aaaacacgtc cagttcaggc aaagagataa ttaatggcga agagtggaac tggcgcagcg
     6661 atgtccattc aagcaaagac ggcacgcttc tggaacgcac cataacggtg acgttaccca
     6721 acggtcagaa aaccgctctg actcgttatc agagcatcca tgatagatcc gggcaggcgc
     6781 aagatgatta atcgccaaca gggtttcaca ttgctggaag tcatggcggc actggcgatc
     6841 ttctcaatgc ttggcgtgct ggcgtttatg gttttttcgc aagcgtctac gctacatcaa
     6901 cgctcacaga aagaaatcca gcagttcaat caactgcaac gcaccatcac cattcttgat
     6961 aacgatctcc tgcaactggt cgctcgccga aatcggagta cggataaaat catggtgttg
     7021 ggtgaagagg ctatttttac cacgcaaagt cgcgatccgc aggccccgct caatgaagcg
     7081 caaaccttac aaacggttca ctggtatctg cgaaatcata cgctttaccg tgctgttcgt
     7141 acttctgtgg atggcaggaa gaatcaacct gcccaggcga tgctggagca tgtcgagagc
     7201 tttcttctgg aaagtaacag cggggaaagc caggagcttc ctctctcagt cacgttacat
     7261 ctgaagactc agcaatatgg ggcgttacag cgccgttttg cattacctga gcagttagcg
     7321 agagaagagt cgccagctca gacgcaggcc gggaataata accatgaata atgaacaacg
     7381 cggcgtagca ctgctcattg tactcatgtt actggcgctc atggctgctc tggctgcaga
     7441 catgacgatc agttttcacg gccagcttca tcgaacccgc caggtaaacc atcatttgca
     7501 acggcaatat gatattgagc tggcggagaa acttgcgctt gccagcctca ccctggacgt
     7561 taaagataac gacagacaaa ccacgttgca gcagttctgg ccacaaccgc aacagttgca
     7621 gctggaaaat ggcaatacag tgaagtggca gttacgcgat gcccagcact gttttaatct
     7681 caatgcctta gcaaaaatct ctgacgcccc gctggcttcc cctgatttcc ccgcccaggt
     7741 attcagtgcc ctgctgatta acgccggtat cgatcgcgga aacaccgatg agatcgtgca
     7801 atcaattgct gactatattg atgtcgatga ctcaccacgc tttcacggcg cagaagataa
     7861 cttttaccaa agccagacgc cgccccggca tagtgccaat cagatgcttt ttctgaccgg
     7921 cgaattgcgt cagataaaag gcataaccga aaacatctat cagcggctta tcccgtatgt
     7981 ctgcgttctc ccaaccacgg aacttagcat caatcttaat atgcttacgg agaacgatat
     8041 tccgctcttc agagcgttat tcctcaataa catcacggat gctgacgctc gggtcttatt
     8101 acaaaaaagg cccagggaag gctggctaac cacggatgca tttctttact gggcgcagca
     8161 ggatttctcc ggcgtgaagc ctctggtagc tcaggtgaaa gggcatctct ttccctatag
     8221 ccgctatttt acgctgagta cggaaagtat cagcgacgaa caatcccagg gatggcaaag
     8281 ccatattttc ttcaaccgta aacagcagag cgcccaaatc tatcggcgta cgctgcaact
     8341 ctactaatca taaggatcgt gatgcctgaa tcgttgatgg tcatacgttc ttcctcgacg
     8401 ctgcgtaagc attgggaatg gatgacgttc tccgcagata gcgtatcttc ggtacatacg
     8461 cccacggacg atcttccgct ggagtcgctg gccgatcagc ccggtgccgg aaatgttcat
     8521 cttttgatcc caccagaggg ccttttatat cgttccctca cgctgcccaa cgccaaatac
     8581 aaactcaccg cacaaacatt gcaatggctg gcagaggaga cgttacccga caacacccag
     8641 gactggcact ggacggttgt cgataaacaa aacgaaagtg tcgaggtgat aggcattcag
     8701 tcggagaaac tcagtcgcta tcttgagcgt ttacatactg ccggactcaa cgtgacgcgg
     8761 gttttgccag atggctgtta tcttccctgg gaagtagata gctggactct ggttaaccag
     8821 caaacaagct ggcttattcg cagtgccgca catgccttca atgaactgga tgaacactgg
     8881 ttacagcatc ttgctgccca gtttccgcct gagaatatgc gttgttatgg tgtggcacct
     8941 cacggcgttg cagcgaccaa tccacttatc cagcatcctg aaataccatc actgagtttg
     9001 tattctgccg atattgcatt ccagcgctat gacatgctgc atggtgtttt tcgtaaacag
     9061 aaaactgtaa gcaaatcggg aaaatggctc gcccgcctgg cagtaagctg tctcgttctc
     9121 gccattcttt cttttgttgg cagccggggc atcgcgctct ggcaaacgct taagattgaa
     9181 gatcaacttc agcatcaaca gcaagaaacc tggcaacgtt atttcccgca gatcaaacgc
     9241 acccacaact ttcgttttta ctttaagcag caactcgctc agcaatatcc tgaagctgtg
     9301 ccgttgctct atcatttaca aacacttctg ctggaacacc ctgaactgca gttaatggag
     9361 gcgaactata gccagaagca aaagtcgctg accttaaaaa tgagtgctaa gagtgaagcc
     9421 aatatcgatc gtttctgtga attaacacag tcctggcttc cgatgcaaaa aacagaaaaa
     9481 gatccggtca gtggtgtatg gacagtaagg agctcaggga aatgattaaa tcatggtggg
     9541 cagaaaaatc cacatcggaa aaacagctcg ttgcggcgct ggcggttctc tcgctcggcg
     9601 ttttttgttg gcttggggtg ataaaaccga tagataccta cattgcagag catcagtccc
     9661 acgcgcaaaa aattaagaag gacatcaaat ggatgcagga tcaagcgagc acccatggtt
     9721 tactcggtca tcctgcgctt acgcagccca tcaaaaatat ccttctcgag gaagcaaaac
     9781 gtgagaattt ggctatcacg ctggaaaacg gtccggacaa tactctgaca attcatcctg
     9841 tcacagctcc cctcgaaaac gtctcccgct ggctcactac ggcacagatt acatacggta
     9901 ttgttattga agaccttcag tttactcttg ccggaaacga acagatcacc ctcaagcatt
     9961 tgtccttcag ggagcaacca taatgacaat gctactgccg ctcttcattc tggttggttt
    10021 tattgcaggt tattttgtta atgccatggc ctatcacctc tcgccgttgg aggataaaac
    10081 ggcgttaacg tttcgccagg tattggttca tttcaggcaa aaaaaatatg cctggcatga
    10141 tacagtgccc ctgatacttt gcgttgctgc tgctatcgcc tgcgccctgg cacctttcac
    10201 gcccatcgtg actggcgcac tctttctcta tttctgtttc gcactcacgc tcagtgttat
    10261 tgattttcgc actcagctcc tgcccgacaa actcacctta ccgctgctct ggcttggctt
    10321 ggtatttaat gcgcagtctg gattaattga tttacatgat gcggtttacg gcgcggtagc
    10381 tggctatggg gtgctgtggt gtgtttactg gggcgtctgg ttagtttgtc acaaagaggg
    10441 attgggctac ggtgatttca agctactggc cgctgcaggc gcgtggtgtg gctggcaaac
    10501 gttgccaatg atactgctaa ttgcctcact gggtggcatt ggttacgcca tcgtttcaca
    10561 acttctgcaa cgccggacta taaccactat cgcattcggc ccgtggcttg cgctcggcag
    10621 catgataaac ctggggtatc tggcctggat ctcgtattaa ccgggagggt tctccctccc
    10681 gacacggctc aactttcttc gcggatctgt gcttgcagat aattttgcag gcccatcttc
    10741 tgaatcagat caagttccgt ttccagccag tcgatatggc cttcttcatc acgcaaaatt
    10801 tctatcatca tatcgcggct gacgtaatca tgaacgctat cggcataacc aattgcctca
    10861 cgcaaattct tcgcgccatc cagctcaagt gccagatcag aacgcagcat ttcctcaaca
    10921 tcctcaccaa tgttcagttt gcccaggtcc tgtaaatttg gaagaccttc cagaaaaaga
    10981 atgcgctcaa tataacgatc ggcgtgtttc atctcatcaa tggattcatg atactccaca
    11041 tcattgagac gtttgagacc ccagttttta aacatccggg catggagaaa gtactgattg
    11101 attgcgacaa gctcatttcc caacagtttg ttgagataat ttataacctt agtatcacct
    11161 ttcatttttt gactccttcg cttccactct ttgaagcgta gaacggctta cgagtaagtc
    11221 aaaaaacaca ctccgtttat gcggactcct taaactccgg caactgcatt aattcatcct
    11281 ccatcacttc acgcgcggcc cggacacatt taccgcactg attgccgacc ggaataaatt
    11341 tttttaattg ctggaacgag tgaggggaaa actggcgcac agcctgacga atttttttat
    11401 cgctgatacc attacaaaga caaacgtaca tagagactcc cgtatacttt cttcaaaaat
    11461 aaatgaaaat agttcttatt tcaattacgg aaagactctt ttttaagcaa agaatagaaa
    11521 tatgaataaa aataatgggc taccaaaagt agcccattga caaaaaatgc ggcgataccg
    11581 gaaggtatcg ccaacacatt tattgcttag taaacggcgc gaagatttcg ctaaatgcgt
    11641 acttcggtgc ctgttcttta gtcaggccgt ggaattccgg agacaggtta gtgccgcctg
    11701 gcaggtcgcg cgcgattgac cagataccaa ccataccgag attacgcttc tgcgcatcct
    11761 gcatgaccag acgagcatca gagagataga acacctcgcc ctgaacgtcg ttcacgccaa
    11821 ccatcggcgt ggtacccatc atagcgtcaa tttctgcatc gctcttattg ggatggaggc
    11881 ctttcaattg tgaatgcagg ttggcaatcg cagacgttgc acacttaccg tgaatgttct
    11941 ggccttcggt atttgcagac tgacaaatcg cgttaccgta gtccattgtc atcacgttca
    12001 cacccgccag ctcaacacct ttcgctttgg catcgctcag gacattcatc ccttccggcg
    12061 tcaggccagt cggcagaatt ggcaaggtgt accagatagc aatatctttg ccctctgact
    12121 tccatttatc ctgcactttc ttcacagcaa ggttacgacg ttcaatagat gcctgatccg
    12181 caacccaggt gccttcgata tcgaagtcca ggactctgag gttcaggtta tcaacgatgt
    12241 cataataatg ctgcatcaga tcgtctacgt tcttacagga agcagccagc ggagcgttgt
    12301 tagcaccacc gatagacagc atcacatcgc cgccagcttc acgcagagct ttgattttgc
    12361 tgtactgagc gtaattctgc ataccgtaag cggtacccca tgtcggcaga caggtgttcg
    12421 catctttact caccacaaac gccagcgtga agtggttgac gttatgattc ttagccagcg
    12481 cagccagatc cgggatcgta ttcagcgtaa agtcaacata cggtgcataa acgtgtttcg
    12541 gccacgcttt cttcggcgta cctacacgtt ctttggttcc ggtccagtca acaaaaacac
    12601 gccacgggtt gctgtcagaa ggagaaactt tctgcacttt actctgagaa atgtagttcg
    12661 caccgttaaa gcgaatcagc gtatcgctgg cataaagcgt ttctggatta aactgcggcg
    12721 cattattcag ctcttcgttg ctatagctct gagccttacc taacggcttc cacgggcggc
    12781 tattgctacc ggtggcgttt tggttcgcaa ccagagctgg gttgtcgctt tgcgtccaga
    12841 acagcgcttc ataagcctgc ccatcaacaa caacgcgatc acctttcacg tacacggtgg
    12901 aagcggacca tgctttcgcc ggttcgtatt tcttccacgg gttatcgccg cccgggacaa
    12961 agccccatgc gtcttgatcg acagaggttt tataatgtgc atcgttataa tctacgatgc
    13021 tgttcgcgct gtacgctttg ctggcctgga aaccatccgc aacaatgacg ccgccattat
    13081 caatttcaca ggtagttggg ttaccgtact gactcatttc cgcagcggta gctgtacgca
    13141 cgagacgcca tgggttattg gagttttctg cactagtgcc acgtgggcaa tcttcagaac
    13201 caacccacca ggcattctga taaacattac cattgaagat aacatggtaa atttcgctac
    13261 cttcctggcc actccacgcc tgcagtgagt aatcgttact gctatccgct ggtgttgatg
    13321 gctcgttatt atccggagta ggagtaactg gagttaccgg tgtttccgga gttggatcaa
    13381 ccggagtcac agcaccgcta ttattatccg gcttaacgga acaggaaccc ggattaccat
    13441 actggctgat ttctgttgcc gttgccgcgc gaacataacg ccatgggttg ctggcatcgt
    13501 tttctttcgc atcgcctggg caatttgccg cgcctaccca ccaggcgttc ttataaatgc
    13561 cgccgttgta gataacgtac catgtagaag aaccttcctg acctttccag gctacgacag
    13621 catagttgtc gtttgactta tcagctggag ctggctgaac atcaggagcc ggtttaacgt
    13681 ccggtgccgg tttcacatcc ggcgcaggct gtggtgctga agtacaggac tgtggattac
    13741 tggtttctga gatttccgtt gccgtagcgg cacgaacata acgccatggg ttgctggcat
    13801 cattgccttt cgcatcaccc gggcaattag aagaggctac ccaccaggca tttttatata
    13861 ccgcaccatt aaagacgaca taccaggtct gaccgccctg ctgtttattc caggcaacta
    13921 cagaagaatt agacggaaca gtgccctgtg ctggtgtagc cgaaccgcca ttagccggcg
    13981 tattggatgc aggcgtattt gaatttgaag aagatgagct gccgctcttt tcgcaggaaa
    14041 gtgtattgcc aaactgacta atttcagcag ctgtcgcggt acgctttaaa cgccatgggt
    14101 tagttgcatc atttgctttt gcttttcccg ggcaatttgt agaagaaacc caccaggcat
    14161 tttcataaat tttgccatcg aaaataacct gatatttatt accaccttgt tggttattcc
    14221 atacttccat tgccaatgct ggcagagcgg aacacaccag ccccata
//
LOCUS       DAZGHF010000121        14043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGHF010000121 DAZGHF010000000
VERSION     DAZGHF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.122"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            143..1033
                     /locus_tag="ACHK3A_003818"
     CDS             143..1033
                     /locus_tag="ACHK3A_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741845.1"
                     /translation="MPPHKIRQEWLTLDEAAEVIRQHNITLLPADLIRHALHGDLSLS
                     VYFPSPVLLRPLNPPCRFACRFSKPPFPPSQCNKPGNRKVMANSPPCRRDPTLQLTTS
                     HLWNLPLIGIERLLLHQQLAKTLNRPCPNDTHCCNQIGIVVCDENDSYYQLCCRKSLR
                     ELLLELLATQNNLQEELHLLIGQLYGNGHNDLKSRLSLSVCYLQDTLPPDALFIIRRT
                     HLEAFLTRHQPESKERITSALSRLLWLACKHNPQLDDEMLEHPYKLLNIFECWAREEG
                     MHLPLSPETLKTALQRGAPP"
     gene            1172..1366
                     /locus_tag="ACHK3A_003819"
     CDS             1172..1366
                     /locus_tag="ACHK3A_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8741846.1"
                     /translation="MNSDRFLRLRQVEDKIGFGKSWIYRQIQLQQFPPSIRLNSRHVA
                     WLESEVDAWIHQRIRLTRDV"
     gene            1383..2666
                     /locus_tag="ACHK3A_003820"
     CDS             1383..2666
                     /locus_tag="ACHK3A_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO8741847.1"
                     /translation="MCASHLLNRWLHGASNTGEQEMVQVSLEQIQPFSLNPRVTRNPG
                     YDVLKASILARGLDNPPVLTRRPGDEKYMLASGGNTRLAILNELWQETQDERYHRVCW
                     PFRPWPETLSPQQGEVQCLIGHLAESDLHNGLMFIERAEGVLHLRDLYLEAGIECPTQ
                     LALAEQLTRDGYPVSQSQISRMLQTVDWLLPCIPNALYGGLTRKVIDRLLSLRSAAEQ
                     VWVKLMPLERQPEFADLFSTALAIFNGEPEAVVPQLVQDELLGEMSCRSGMSWNTLLA
                     ELTDGQSKRQVLLGPPAEKVLWPPPPEVEKAPLPEPEPPTEPAAPDEPKPSPLVHTAQ
                     SLCACWGLDACIQPDDSAPAGFTLAPCHDFPHPAAQRCYQVLAALNGDTSTPMPALEL
                     LLSPDYNNAQIQQLMQLISLCRQRRLREVRHDTLS"
     gene            2650..3207
                     /locus_tag="ACHK3A_003821"
     CDS             2650..3207
                     /locus_tag="ACHK3A_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO8741848.1"
                     /translation="MTPSVNQAVLTHIVQALKEGQIRYCESLGFSPQELCELTRLTAD
                     DILFLSNSAVQFITTHIDHEMLGRMLARMEQERLFQQRLEEALSLGASIEFINHYFGL
                     STPEVCARRRLIGLFVRQGRNNAPDEQVETLVWNEWQKTGVKKLDSPEALTAMMAMAR
                     EHDLSLTVIWNLLRQWTQEKLLHEE"
     gene            3197..4069
                     /locus_tag="ACHK3A_003822"
     CDS             3197..4069
                     /locus_tag="ACHK3A_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO8741849.1"
                     /translation="MKSDDRLLFLGNPQQSVPKRLLLARNLSPRDKFAWQLLRLHARD
                     DGSGVFPSYNELQGWLSDQPEGEKASRSTVSNALMMLRITRWLSLCQRLRDEQSGRIT
                     GNLYALHDAPLTHADACRMDSEYLSLLTACSRHRNRRIRNIAIGLLLTLQQPVDGGRT
                     CTESPLKTAPGLNRGLSKKCPSLQLRLSNVAGAGGQPEPHCVHTGTDLNNKHQKRTPD
                     LHWPVDNPFSASERRAAERAMRNLDPALCQQVLDDCLWRIAAHDIHRPLAYLLATLEK
                     ARRGEFNQLRRRRR"
     gene            4229..4840
                     /locus_tag="ACHK3A_003823"
     CDS             4229..4840
                     /locus_tag="ACHK3A_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO8741850.1"
                     /translation="MPGTTLFSDIRITLHTRVAARLWQAHPTGMLLCLALLRRLLRAE
                     EADDPWAAHWLKQLRIRLNLIAHLLKQKNRRLDHAFASLPGAIHTTQVSNPSPTEFIL
                     PLALFSPPGSRLLQQLIDYDLLVRRTLLAWHLGLITQAEKRDFIATIPRLMLQVFSFV
                     NRFRTTGVTRADVRANSPLAQSMAHKLGNLPKKMLTEIQRDVQ"
     gene            4830..5255
                     /locus_tag="ACHK3A_003824"
     CDS             4830..5255
                     /locus_tag="ACHK3A_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HFO8741851.1"
                     /translation="MFNDDDDERSARKSSLKGLLKAFKGKGELRALVQELQALRLQLQ
                     QNQPVLKQAVYELPGRNVPLILCSNPARSGVSYLRWRNLDNNRSGSVALQEAIQQPDA
                     SDELRDALMALEWRRHVLNTQLSVVMFMLRRIQPLTTLV"
     gene            5447..7252
                     /locus_tag="ACHK3A_003825"
                     /pseudo
     CDS             5447..7252
                     /locus_tag="ACHK3A_003825"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            7360..7542
                     /locus_tag="ACHK3A_003826"
     CDS             7360..7542
                     /locus_tag="ACHK3A_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011094427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741852.1"
                     /translation="MAKNTGDGYRKGSVTDRTQVQNPQNGNYTKRDTTTGRFIDQKQD
                     GTPFKGVAKEVDDRRK"
     gene            7826..8269
                     /locus_tag="ACHK3A_003827"
     CDS             7826..8269
                     /locus_tag="ACHK3A_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO8741853.1"
                     /translation="MNDTHYFNLYTSGLGYLNRIREVTPKKGEPFLCCDIAALRGEAS
                     NAEHTWFDCRVSGKEAQKLVRRCIDACEQKRKVLIQFRLGDLYADTFVYSKGDRKGET
                     GVSLKAHLLHIGLIKIDGEEVYRAEREAEPQPAPAALPAEPVAQF"
     gene            8343..8723
                     /locus_tag="ACHK3A_003828"
     CDS             8343..8723
                     /locus_tag="ACHK3A_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741854.1"
                     /translation="MNTVLNSREEALDRGKLINLDDEALDAGLPLDLAITLPAHKHCL
                     EDRNDNAIRDLLKGVEMAAVVAFISHQPPQRTIRIDYRSVLTQKTNGSTQQQTVRLTL
                     RVHFSEQGIPIAATLGLMGERFPY"
     gene            8837..9493
                     /locus_tag="ACHK3A_003829"
     CDS             8837..9493
                     /locus_tag="ACHK3A_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilL"
                     /protein_id="HFO8741855.1"
                     /translation="MKRLLTALLLFIFNGVAASPATPEVVRYDRYLLVSTSPQQPPLE
                     QLTTLSVPPGFHPTVGEALQYLLRDSGWSLCLSDAHRAQLYGLPLPLNQYHTGPLRLK
                     AALQLLAGPAWRMTADENRREVCFTSDHSLPQTVAYRHPVSLHPLGPLTAPFFLAGTV
                     QRGDIRLAVIVPPEALYLHQVVWLRIGEQWHGWRLARFDERRAVFRHGKRQLTLKVPE
                     "
     gene            9497..10171
                     /locus_tag="ACHK3A_003830"
     CDS             9497..10171
                     /locus_tag="ACHK3A_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO8741856.1"
                     /translation="MKRLILLLLFTASTWAQPLQVQTSQNVGSALQPLTTQQQATVWG
                     ITPEEWQRYLTLKQQARGVWSPGLDPLTTLGVEADTDHERQRFAELLVRQEYQRLEKE
                     LAFQRAYDAAWQRLYPGLTPLKTTSASTSRVSLFVKDDCPPCDSLLRTLLAQQRPLDI
                     WLVGSEGDDNRVRRWAMAHGIDAKLVGEHSITLNHDAGRWLLLGQSKIPVALERKGEQ
                     WREVLP"
     gene            10150..10668
                     /locus_tag="ACHK3A_003831"
     CDS             10150..10668
                     /locus_tag="ACHK3A_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO8741857.1"
                     /translation="MARGPALILLLPCLALAQPLPEAYLRTAEHFRVPPELLWAVTQT
                     ESGTNLSGRHLPWPWTLNISGTGYRYASREQACQALLVAIRRTSPKRIDVGLGQINIG
                     WHQAYFDSPCDALYPLQNLSLTAQLLRQRYDERPGSWLNAAARYHRPAGGAPAARYRK
                     LVSRHLQQASPP"
     gene            10665..12683
                     /gene="traD"
                     /locus_tag="ACHK3A_003832"
     CDS             10665..12683
                     /gene="traD"
                     /locus_tag="ACHK3A_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO8741858.1"
                     /translation="MSRYALEALLRPPVEFYSTVIAGCGALICLTQAPLLMLTPWQAR
                     MMASALMLLALRRGWQGWQIVRYQRNLLRLPRYALTSRKIPVSRQRLFLGKGFRWQPI
                     HTQRLYDCLQPEAARWLRPGWCYRLARHIEKRSEHTLPGLARLLSADRWLNPVRPLPP
                     VGGNPWLHGVELKECDVTLPLSERPGHTLVLGTTRVGKTRLAELLATQDIRRGEVVIV
                     FDPKGDADLLKRMWTEAQRAGRGDRFILFHLAYPEFSARYNAIGRFGRISEVASRLTG
                     QLSGEGNSAAFREFAWRFVNIIARALVALGQRPDYQLISRHVVNIDALFIDYARIVLP
                     RHNTRSWEVVVQLAERVNDKNTPRHMLGRDPHVVALEQYLNAEKLYDPILDGLLSAVR
                     YDKTYFDKIVASLLPLLEKLTTGTLAALLAPDYHDLDDARPILDWQQAIRQKAVVYVG
                     LDALSDSVVAAAVGNSMFADLVSVAGHLYKFGLNDGLPGNSEKPPPINLHCDEFNELM
                     GEEFIPLVNKGGGAGIQVTAYTQTLSDIQARIGSAPKTGQVVGNFNNLIMLRVRETAT
                     AELLTKQLPQVEVRSRQVSSGVTDTPGGINGFTSNTCEQVTTRNVPLLDTAALIQLPK
                     GQAFALLEGGQLWKIRLPLPVADKQCPLPDSVAQLAAWMQARQQGLTE"
     gene            12680..13327
                     /locus_tag="ACHK3A_003833"
     CDS             12680..13327
                     /locus_tag="ACHK3A_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFO8741859.1"
                     /translation="MSELRPPPSRPPQGPIGWLLSLAGRIIGLVIGTLVLRVVLELVG
                     LYFWWPQEGSRHVFQVMRQEKVELIIAVQPHPLGKEIRVLLEHGITRVNDVRHKAVSR
                     LFHEPVTVLAYTMISFALRLTWLVIMLPLLCLSAVTGLTEGLVHRDLRRFGSGLESIF
                     LHRYVIRCGSSVTTTLWVVYLAQPFSLPAMLVLLPAALWSGIAVWMAAGSFKRWL"
     gene            13393..13638
                     /locus_tag="ACHK3A_003834"
     CDS             13393..13638
                     /locus_tag="ACHK3A_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO8741860.1"
                     /translation="MGVMSVRLNNNLSTQLEALSKATGRSKSWLANQAIEDYVAREAW
                     QVAEIEQAIQEADAGDFATSDEVDKLFQRLGVKPDGN"
     gene            13628..13912
                     /locus_tag="ACHK3A_003835"
     CDS             13628..13912
                     /locus_tag="ACHK3A_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8741861.1"
                     /translation="MEIKWLRKAIANLEAEFRYIAEDDPQAAERFVNEVWRLTQLLKE
                     QPAMGREGRVPETRELILHNYPYIIPYRVRNNIIQVLRVFHTHRRLPVNW"
BASE COUNT         3046 a         4345 c         3897 g         2755 t
ORIGIN      
        1 tttcctaaat aaaacagtaa aaaacctaaa aaaaacacca atagatcaca tacaaaacaa
       61 ccagttaaaa gaactcacct tacctcgctt tagttggctt ttttagatag tactcatcat
      121 atgtaactaa taggagttac ccatgccccc acacaaaatt cgccaagagt ggctgacgct
      181 ggatgaggct gcagaagtca tccggcagca caacatcacg ttgctgcccg ccgacctgat
      241 ccgccatgcc ctgcacggcg atctgtcgct ctcggtctac ttcccttcgc ccgttctgct
      301 gagaccactc aatccgcctt gccgctttgc ctgccgattc agtaagcctc cttttccacc
      361 ttcgcagtgc aacaaacccg gcaaccgcaa agtgatggca aactcgccgc cgtgcagacg
      421 cgacccaact ctgcaactga ccaccagcca tctgtggaat ttgccgttaa tcggcattga
      481 gcgcctgctg ctccaccagc aactggcgaa aacgttgaat cgcccttgcc ccaatgatac
      541 ccattgctgc aaccagattg gcatcgtggt ctgcgatgaa aacgacagct actaccaact
      601 ctgttgccgc aaatcgttgc gggagctttt actggaactg ctcgccacgc aaaacaacct
      661 ccaggaagag cttcatctgc tgattggcca gctatatggt aatggccaca atgacctgaa
      721 atcccggctc agtctgtccg tctgctacct gcaggacacg ctgcctcctg acgcactgtt
      781 catcatccgc cgcactcatc tcgaagcctt cctgacccgt catcagccag aatcaaagga
      841 gcggatcacc tccgcgcttt cgcgtttgct gtggctggcc tgcaagcaca acccgcaact
      901 ggacgatgaa atgctggagc atccctacaa actgctgaat attttcgaat gctgggcgcg
      961 tgaggaaggt atgcacctgc cgctgagccc cgagacgttg aaaaccgcac tccagcgcgg
     1021 cgcaccgccg taacgcgatc aactaaccct ttcaactaac aactctcact aacccagata
     1081 aggattttta ctgcctggca gggacgctgc tgctttgata accacgccta tcgtgctgat
     1141 gtgaacgaat ctggacacag gaggaatggt tatgaatagc gacagatttt tacgcttacg
     1201 ccaggtggaa gataaaatcg gcttcggtaa atcgtggatt tatcgacaaa tccagctgca
     1261 acagtttccg ccgtccattc gactcaatag ccgccacgtc gcctggctgg aaagcgaggt
     1321 ggacgcgtgg atccaccagc gcatccggct tacccgcgat gtctgaatgc cggaggaaga
     1381 ttatgtgcgc ttcacacctg ctcaaccgct ggcttcacgg cgccagcaat accggagaac
     1441 aggaaatggt ccaggtcagc ctggagcaaa tccagccgtt ctccctcaat ccccgcgtca
     1501 cccgcaaccc cggctatgac gtgctgaagg cgtcaattct tgcccgtggg ctggataacc
     1561 cgccggtact gacccgccgc cccggtgatg aaaaatatat gctcgccagc ggcggaaata
     1621 cgcgcctcgc catcctcaat gaactgtggc aggagacgca ggatgagcgt taccaccgcg
     1681 tctgctggcc gttcagaccg tggccggaaa cgctgtcacc gcagcaaggc gaagtccagt
     1741 gcctgatcgg ccatctggcc gaaagcgacc tgcataacgg attgatgttt atcgaacggg
     1801 cggaaggggt cctgcacctg cgcgatcttt acctcgaggc aggcattgaa tgcccgacgc
     1861 agctggcgct ggcggaacaa ctcacccgcg acggctatcc cgtcagccag tcgcaaatca
     1921 gccggatgct acagaccgta gactggctgc tgccctgcat cccaaacgcg ctatatggcg
     1981 gtcttacccg caaggttatc gatcgcctgc tgtcactacg cagcgcagca gaacaggtct
     2041 gggtaaagct gatgccgctg gaacgccagc ccgaatttgc cgacctgttc agcaccgccc
     2101 tcgccatctt caacggcgag ccggaagccg tggtaccgca actggtgcag gacgaactgc
     2161 tcggtgagat gagctgtcgc agcggcatga gctggaatac gttgctggcg gaactgactg
     2221 acggtcagag caagcggcag gtgctgctgg ggcctccggc cgaaaaggtc ctctggccgc
     2281 caccgccgga agtggaaaaa gcaccactac cagagcctga gccaccgact gaacccgccg
     2341 cgcccgatga gccaaagccc tcaccgctgg tgcatacggc acagtcgctg tgtgcctgct
     2401 ggggactgga cgcctgtatc cagccggacg acagcgctcc ggccggtttc accctcgcgc
     2461 cttgccacga ttttccccac ccggcggcac agcgctgcta tcaggtgctg gcggccctga
     2521 atggcgacac cagtacgccg atgcccgccc tcgaattgtt gctgtcgccg gactacaaca
     2581 acgctcagat ccagcaactg atgcagctta ttagcctgtg ccgccagcgg cggctgcggg
     2641 aggtgcgcca tgacaccctc agttaaccag gcagtactca cccacatcgt ccaggcgctg
     2701 aaggaggggc agatccgcta ctgcgaatcg ctcggcttca gcccgcagga gctgtgcgag
     2761 ctgacgcgcc ttaccgccga cgacatcctg tttctcagca acagcgcggt gcagttcatc
     2821 accacccaca ttgatcacga gatgctgggc cggatgctcg cgaggatgga gcaggagcgg
     2881 ctgttccagc agcgtctgga ggaagcgctg tcgctcggcg cgtccatcga gttcattaac
     2941 cactattttg gcctttccac gccggaggtc tgtgcccggc ggcggctgat tggcctgttt
     3001 gtccgccagg ggcgcaacaa tgccccggat gagcaggtgg agacgctggt gtggaacgag
     3061 tggcagaaga cgggcgtgaa aaagctcgac tcacccgaag cgctaaccgc catgatggca
     3121 atggcccgcg aacacgatct gtcgctgacg gtcatctgga acctgctgcg ccagtggact
     3181 caggagaagc tgctgcatga agagtgacga tcggctgctg tttctcggca acccgcagca
     3241 gagcgtgccg aagcgcctgc tgctggcgcg caacctctcc ccgcgcgaca agttcgcgtg
     3301 gcaactgctg cgcctgcatg cccgcgacga cggcagcggc gtcttcccct cctacaacga
     3361 actgcagggc tggctctctg accagcctga aggcgagaag gcctcgcgca gtaccgtcag
     3421 caatgcactg atgatgctgc ggatcacccg ctggctgagc ctgtgccagc ggctgcgcga
     3481 cgagcaaagc ggcaggatca ccggcaacct ctacgccctg catgatgcgc cactgactca
     3541 tgccgatgcc tgtcggatgg acagtgaata cctgtcgctg ctcaccgctt gttcccgcca
     3601 ccgcaaccga cgcatccgaa acatcgctat cgggctgctg ctcaccctgc aacaacctgt
     3661 ggacggtgga cgaacctgca ctgagtctcc attgaagacc gcccctggtc ttaatcgagg
     3721 actcagtaaa aaatgcccga gtcttcaatt aagactcagt aatgtggcgg gggctggcgg
     3781 gcagccggag ccgcattgcg tacatactgg tacagattta aacaataaac atcaaaagcg
     3841 tacgccagac ctgcactggc ctgtggataa tcccttcagc gccagcgagc gccgcgccgc
     3901 agaacgcgcg atgcgcaatc tggatcccgc tctctgccag caagtgctgg acgactgcct
     3961 gtggcggatc gccgcccacg atatccaccg ccccctcgcc tatctgctgg caacgctgga
     4021 gaaagccaga cggggcgagt tcaatcagct tcggcgcagg cgcagatagc cctgatttta
     4081 tgcgcaccgc ataaagccgg tgtaccaact atcaatcaaa cctcattcat cggcgaaatt
     4141 aactttctgc ggaccagatg ggtttcccgt cgcctgactc attgtgcgga ccgacactgc
     4201 gggcatctga atccatggag aatttccgat gcctggcaca accctgttca gcgatatccg
     4261 catcaccctg catacccgcg tcgccgcccg gctgtggcag gcgcatccca ctggcatgct
     4321 gctctgtctg gcgttactca ggcgtctgct gcgcgccgaa gaggccgacg atccgtgggc
     4381 cgcccactgg ctgaagcaac tgcgcatccg cctgaacctg atcgcccacc tactgaagca
     4441 aaaaaaccgt cgtctggacc atgctttcgc cagcctgcca ggcgctatcc acaccacgca
     4501 ggtcagtaac ccgtccccga ccgaattcat cctgccgctg gcgctattct caccgcccgg
     4561 ttcgcgcctg ctgcaacagt tgattgatta cgatctgctg gtccgccgca ccctgcttgc
     4621 ctggcatctc ggccttatca cccaggcgga gaagcgcgat ttcattgcca ccatcccacg
     4681 cctgatgctc caggtgttca gcttcgtgaa ccgctttcgc accactggcg tgactcgcgc
     4741 cgacgtgcgg gcgaacagtc cgctggcgca gtcgatggcg cataagctcg gaaatttgcc
     4801 caaaaagatg cttacggaga tacagcgcga tgttcaatga cgatgatgat gaacgctccg
     4861 cccgcaaaag ctcgctaaag ggccttttaa aggcctttaa gggtaaggga gagctgcgcg
     4921 cgctggtaca ggaattacag gcgctgcgct tgcagctgca acaaaatcag ccagtgctga
     4981 aacaggcggt gtatgaactg ccggggcgta atgtaccgct gattctgtgc agcaacccgg
     5041 cgcgctccgg cgtcagctat ctgcgctggc gcaatctgga caacaaccgt tcggggagcg
     5101 tggcgttgca agaggccatt caacagccgg acgcgagcga tgaactgcgc gacgcgctga
     5161 tggcgctcga atggcggcgg catgtgctga atacgcagct ttcagtggtg atgtttatgc
     5221 tgcggcggat acaaccgtta accaccctgg tttaaaattt tgcgcaaagt taaaccctga
     5281 gtgtcacttt atcgattatt ttaaacttaa ctcatgcacc ttaaaacagg cactgtgcgc
     5341 ttaatgcacg ttttaaggtg caatttttac cttctcctac cagtttcccc tgtcgcctta
     5401 tccctccggc acaccatgct gaactccctt tcaccggaga cagcccatgc aactttacct
     5461 gtgcgaaaaa ccctctcagg cacgcgacat cgcccgaatt cttgacgccc ataaccgcag
     5521 cgacggctac ctgcataacg ccaccgtaac catcacctgg tgtttcggcc atctgctgag
     5581 catggccgcg ccggaggatt acgatccggc gctgaaacac tggcggacgg aacagctgcc
     5641 gtttattccc ccggcatggc ggttggtgat ccgcccggag gtgcataagc agttccgtat
     5701 catcgaaaag ctgctgaaac aggcggatgc agtggtgatt gccaccgacg ccgatcgcga
     5761 aggcgaaacc atcgcccgcg aaattctgga acaatgccgc tggcgcggtc cggtccagcg
     5821 gctgtggctg tcggcgctgg atgaggccag tatccacaag gcgctcgcca ctctgcgtcc
     5881 cggcgacagc acctttccgc tgtatcaggc cggactggcg cgcgcccgca ccgactggct
     5941 ggtcggcatc aacatgaccc gcgcctgcac gctaatgaac cggcggcaca agggcgtgct
     6001 ctccgtgggc cgggtgcaga cgccgaccct gcgtctggtg gtggagcgcg accgggagat
     6061 cgcccgcttc acgccccgcc cctggtggcg ggtggatgcc cgtttgcatg cggagggttg
     6121 ttcctttctg gcgcagtggc agccggatag tgcgtactgt gacgacgaaa gccgctgcat
     6181 ccgcgaatcc gccgcccgtg aggccacagc acgcctgcaa caagccggaa ccgcgctggt
     6241 aatggacgtg acaacccggc gggagaaaac gccgccaccg caggccttca ctctcagcgc
     6301 cctgcagcag gcctgctcaa aacagtgggg catgggcgca caggaggttc tggatatcgc
     6361 ccagctcctg tatgaaaccc ataaagccac cacctacccg cgcaccgact gcggctatct
     6421 gccgctctcc atgcacgatg aagcaccaca agttctggcg gcgctggtcg ccagcgacgc
     6481 ttcactccag cccctcgcca cgcagcttaa tctgcagcaa cgctcccggt tgtgggacga
     6541 cagcaaaatc accgcccacc atggcatcat cccgacccgt aaaacggtag atctgacgca
     6601 gatgaacgac agcgagcgca aggtctacgg cctggtacgc agccattttc tggcgcagtt
     6661 tctgccagac cacgaagtgg ataaaacgga cctgcgcctg aactgcgccg gggaaacgct
     6721 gactgcccgt ggctcagtgg tgattgtcag cggctggcga caactgtttt tgcgcgaaat
     6781 ggcaacgcac tcaccggaag aaacgcaggc acttcccgcc ctgcaacagg gacaaacctg
     6841 ctaggtggaa caggtggacg ttcgcgcccg ccagacccag ccaccggagc actacaccga
     6901 agggacgctg attgccgcga tgaaaaatgc cgcccgtttt gtcagcgatg aacgcctgaa
     6961 acagcggctg aaagaaaacg cgggcatcgg caccgaagcc acccgcgccg ggattatcca
     7021 gacgctcctt aagcgcggct atctgattaa gcagcgtcgt ttcctgctgg caacggatac
     7081 cgccagcacg ctgattgacg ccctgccgga aacgctgaaa gatccaggca ccactgcact
     7141 gtgggagcaa atgctggatg acatcgcgac ggggaaaatg ggactggagg cgttcctggt
     7201 gcagcaacag cagaacgtga cagagctgat taagtcgtta cggaatgagt aaataacctg
     7261 tttcctgcat cacactttta cttttccctg attgctcgtt caaattggca gtctgatgat
     7321 cctctctgag gaatattcct catttttcgg agtgttaaga tggccaagaa taccggagac
     7381 ggttatcgca agggctctgt tacagaccgc acacaggttc agaatccgca gaatggcaac
     7441 tacaccaaac gcgatacaac gacaggtcgc tttatcgacc agaagcagga cggcactccc
     7501 tttaaaggtg tcgccaaaga agtcgatgac cgccggaaat aatatttagc taactttcag
     7561 ggccttagtt attaaggcct ttttgcattc tgtacttcca actttcacgc cgtaaaaaca
     7621 attccacctt tcgtttctcc ctaccgaaga ttcaactaaa caggtcaacc ccaaaacccg
     7681 ccatgcattc ctgaaacgcg gaggcgcaca gcgcggaatg cccgtactgc cagtaactca
     7741 gaggccttgc agccaatgag ccaaacccga agcggggcgt tcccgcgcat ggggcgctcc
     7801 gcaccttcaa cacggagata ccaccatgaa cgatacccac tacttcaacc tgtacaccag
     7861 cggtctgggc tacctcaacc ggatccggga agtgaccccg aaaaaaggcg aaccgttcct
     7921 gtgttgcgac attgccgcac tacggggcga agccagcaac gcggagcaca cctggttcga
     7981 ctgccgcgtc agcggcaagg aagcgcagaa actggtgcgc cgctgtatcg acgcctgcga
     8041 gcagaaacgc aaggtgctga tccagttccg cctcggcgat ctgtacgccg acacctttgt
     8101 ctacagcaaa ggcgaccgta aaggcgaaac cggcgtcagc ctgaaggccc atctgctgca
     8161 catcgggctc atcaaaattg acggtgaaga ggtttaccgg gcagagcgcg aagctgaacc
     8221 tcagccagcg ccagccgcac tgccagcaga gcctgtagcg cagttctgac gcacagcatt
     8281 caccctgccg gggaaattcc ccgtcggggc gtttccctgg ccttttaccg gagagacgca
     8341 cgatgaatac cgtattgaac tcacgcgaag aagcccttga ccggggcaaa ctgattaacc
     8401 ttgatgacga ggcgctggac gctggcttgc cactggatct ggccattacc cttccggccc
     8461 acaagcactg tcttgaagac cggaatgaca acgccatcag ggacttgctg aaaggtgttg
     8521 aaatggcagc ggtagtggcc tttatctccc accaaccgcc gcagcgcacc attcgtatcg
     8581 attaccgctc ggtgctgacg caaaaaacca acggcagcac gcagcagcaa accgtcagat
     8641 tgacgttacg cgttcatttt tctgagcaag gcatccccat cgccgccacg ctggggctga
     8701 tgggggaaag attcccttac tgatctgatt ttgcgcccgg aataacgggc gctatttttt
     8761 gacgcagcaa acagttgtcg gtagtcccca gccctctcat ccggcacgct cctgtaaaag
     8821 cactgtggag gatgcgatga aacgactgct gacagctctg ctcctgttta ttttcaacgg
     8881 cgtggccgcc tcccctgcaa cgccagaagt cgtccgctac gaccgctatc tgctggtcag
     8941 cacgtcaccg caacaaccgc cactcgaaca gcttaccacc ctgtccgttc cgcccggttt
     9001 tcacccgaca gtcggtgaag cattgcagta cctgctgcgc gacagcggct ggtctttgtg
     9061 tctctccgat gcgcaccggg cgcagcttta tggcctcccg ttaccgttga accagtacca
     9121 caccggcccg ctgcgcctga aggccgccct gcaactgctg gcaggcccgg catggcgaat
     9181 gaccgcagac gaaaaccgcc gtgaagtctg ttttaccagc gaccattccc tcccccagac
     9241 ggtggcttac cgtcatcccg tgtccctcca ccctttgggg ccgttaacgg ccccgttttt
     9301 tctcgccgga accgtacagc gcggcgatat ccgactggca gtgatcgtgc cgccggaggc
     9361 cctttatctc catcaggtag tgtggctgcg tatcggggag cagtggcacg gctggcggct
     9421 ggcgcggttt gatgagcgcc gggcggtatt ccgccacggt aaacgccagc tgacgctgaa
     9481 agtaccggag taagcgatga aacgcctgat tctcctgtta ctgttcaccg ccagcacgtg
     9541 ggcgcaaccg cttcaggtgc agaccagcca gaacgtgggt tcagccctgc aaccactgac
     9601 cacgcagcag caggccaccg tctggggcat cacgccagag gagtggcagc gctatctcac
     9661 gctgaaacag caggcgcgcg gcgtctggtc gccggggctc gatccactga ccacactcgg
     9721 cgtagaagcc gacactgacc acgaacgcca gcgctttgcc gaactgctgg tcaggcagga
     9781 gtaccagcgg ctggagaaag aactcgcctt ccagcgtgct tatgatgccg cctggcagcg
     9841 cctgtatccg ggcctgacgc cgctgaaaac cacctctgcc agtacttccc gcgtttcgct
     9901 atttgttaaa gatgactgcc cgccctgcga cagcctgctg cgtaccctgc tggcgcaaca
     9961 gcgcccgctg gatatctggc tggttgggag tgaaggcgat gataaccggg tgcgccgatg
    10021 ggcgatggcg catggcatcg acgcaaaact ggtgggtgaa cacagcatca cgcttaacca
    10081 cgacgcgggc cgctggctcc tgttggggca gagcaaaatc ccggtggcgc tggaacgtaa
    10141 gggggagcaa tggcgcgagg tcctgccctg atcctgctgc taccctgtct ggcgctggca
    10201 cagccgctgc cagaagcgta cctgcgcacc gcggagcatt tccgcgtgcc gccggagctg
    10261 ctgtgggccg tcacacaaac cgaaagcggc acgaacctga gcggcagaca tcttccgtgg
    10321 ccgtggacgc tcaatatcag cgggaccggc taccgctatg ccagccggga gcaggcctgc
    10381 caggcgctgc tggttgccat ccgccgcacc agcccaaagc gcattgacgt gggactcggg
    10441 caaatcaaca tcggctggca ccaggcatat tttgactctc cctgcgacgc cctgtatccc
    10501 cttcaaaacc tgtcgctcac cgcgcaactg ctgcgtcagc gctacgatga acgtcccgga
    10561 agctggctca atgccgccgc ccgctatcat cgtcccgctg gcggcgcgcc tgccgcgcga
    10621 taccgcaagc tggtcagccg ccatctgcaa caggcctcac cgccatgagc cgctacgcgc
    10681 tggaggcgct gctgcgccca cccgtggagt tttacagcac cgtcatcgct ggctgtggcg
    10741 cattaatctg cctgacgcag gccccgctgc tgatgctgac gccgtggcag gcccgcatga
    10801 tggccagtgc cctgatgctt ctggccctgc ggcgcggctg gcagggatgg caaatcgtac
    10861 gctaccagcg taacctcctg cgcctgccgc gctacgcgct caccagccgg aaaatccccg
    10921 tcagccgcca gaggctgttt ctcggtaaag gctttcgctg gcagcccatc cacacacagc
    10981 gtctgtacga ctgcctgcaa ccggaagccg cacgctggct gcgccccgga tggtgttacc
    11041 gcctggcaag acacatcgaa aaacgcagcg agcataccct gcccggcctt gcccgactgc
    11101 tcagcgccga tcgctggctg aacccagtac gtccgctgcc acccgtaggc ggcaatccgt
    11161 ggttgcatgg ggtggaacta aaagagtgcg acgtcactct gccgctcagt gaacgccccg
    11221 gccatacgct ggtgctcggc accacccgcg ttggcaaaac ccggctggcg gaactgcttg
    11281 ccactcagga catccgccgt ggcgaggtgg tgatcgtttt tgatccgaaa ggcgacgcgg
    11341 atctgctgaa gcgtatgtgg acagaagcgc agcgcgccgg acgcggcgac cgctttatcc
    11401 tgttccatct ggcgtacccg gagttctccg cccgctacaa cgctatcggg cgatttggcc
    11461 gcatttcgga ggtggcgtct cgcctcactg gtcagctttc cggcgaaggc aactcggcgg
    11521 cctttcgcga gttcgcctgg cgctttgtca acatcatcgc ccgcgccctg gtggcgctcg
    11581 gccagcgccc cgactatcag ttaatttccc gccacgtcgt caatatcgac gccctgttta
    11641 tcgactatgc ccgcatcgtg ctgccgcgcc acaacacgcg gtcatgggaa gtggtggtgc
    11701 aactggcgga gcgcgttaac gacaaaaaca cgccgcgcca catgctgggc cgcgatcctc
    11761 acgtggtggc gctggagcag tacctcaacg cggaaaaatt atacgatccg atcctcgatg
    11821 gcctgctctc ggcggtgcgc tatgacaaaa cctatttcga caagattgtc gcctcactgc
    11881 tgccactgct ggaaaagctg accaccggca cgcttgccgc cctgctggcc ccggattacc
    11941 acgatctgga cgacgcgcgt ccgatcctcg actggcaaca ggcgatccgc cagaaggcgg
    12001 tagtctatgt cgggttggac gcgctctccg attcggtggt cgctgccgcc gtcggcaatt
    12061 cgatgtttgc cgatctggtg agcgtcgccg ggcatctgta taaatttggc ctcaatgatg
    12121 gactgcccgg caatagcgaa aaaccgccgc ctatcaatct gcactgcgat gagtttaatg
    12181 aactgatggg cgaggagttt attcccctgg tcaacaaggg cggcggtgcg ggcattcagg
    12241 tcaccgccta cacgcagacg ttgagtgata ttcaggcccg tatcggcagc gcaccaaaga
    12301 caggccaggt ggtgggtaac ttcaacaacc tgattatgct gcgggtacgc gaaaccgcca
    12361 ccgccgaact gctgacgaag caactgccac aggtggaagt gcgcagccgc caggtcagtt
    12421 caggtgtcac cgacacgccg ggcggcatca acggctttac cagcaacacc tgcgagcagg
    12481 tgaccacccg caatgtaccg ctgctggata ccgccgcgct gatccagctc cccaaaggcc
    12541 aggcttttgc cctgctggaa ggcgggcagt tgtggaaaat tcgtctgccg ctgcccgtag
    12601 cggataagca atgcccacta ccagattctg tcgcgcaact ggcggcgtgg atgcaggcgc
    12661 gccagcaggg gctaacggaa tgagtgagct gcgaccgcct ccgtcacgcc caccacaggg
    12721 accgattggc tggctattgt cactggccgg acggattatt gggctggtta tcggtacgct
    12781 ggtgctgcga gtggtgctgg aactagtggg gctatatttc tggtggccgc aggaagggag
    12841 caggcatgtt tttcaggtga tgaggcagga gaaggtggaa ctgattattg cggtacagcc
    12901 acatcctttg gggaaagaga tacgagtact gctggagcat ggaataaccc gcgttaatga
    12961 tgtcagacat aaggccgtga gtcggttgtt ccacgaaccc gttaccgtcc tggcttatac
    13021 catgatcagc tttgcgctgc gcctgacgtg gctggttatc atgttgccac tgctgtgcct
    13081 cagtgctgta accgggctga cagaaggcct ggtgcaccgg gatttaagac ggtttggctc
    13141 cgggctggaa tccatttttc tccaccgtta tgtcatcagg tgcggcagtt cagtcaccac
    13201 cacgctttgg gtggtttatc tggcgcagcc attttctctg cccgcgatgc tggttttact
    13261 tcctgccgcc ttatggtccg ggatagcggt atggatggca gcaggaagct ttaaacgttg
    13321 gttataagtt gctcaaaaaa tcgcacctca ctacaatgta tacaatgtgt acatgtgtat
    13381 gaggccacca atatgggcgt aatgtcagtc cgtttaaaca acaatctttc cacgcaactg
    13441 gaagcattat caaaagcgac cggacgctca aagtcctggc tggcaaatca ggcaattgaa
    13501 gattatgttg cccgggaggc ctggcaagtt gcagagattg agcaggccat tcaggaagct
    13561 gatgccggtg attttgccac ttcagatgag gtggataagc tgtttcaacg tctgggagta
    13621 aagccagatg gaaattaagt ggctacgtaa agccattgcc aatctcgaag ctgagttccg
    13681 gtatattgct gaagacgatc cgcaggctgc ggaacggttt gtgaacgaag tctggcgact
    13741 tacgcaactg ctcaaagagc aacccgctat gggccgggaa ggaagggtac ctgaaacacg
    13801 tgagctcatt cttcataatt acccttatat cattccctat cgagtacgaa ataatataat
    13861 tcaggtttta cgcgtgtttc atacccatcg gcgcttacct gtaaattggt gataaaaaac
    13921 acaagacaag aaatttttta gagatggaat tcaaaccatg gacaaaccta tttttaatat
    13981 cataaacaaa aaattcatta tttcagtagt aacctcatct accttcatca ctggattcac
    14041 gat
//
LOCUS       DAZGHF010000122        13849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGHF010000122 DAZGHF010000000
VERSION     DAZGHF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.123"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1128
                     /gene="tssD"
                     /locus_tag="ACHK3A_003836"
     CDS             <1..1128
                     /gene="tssD"
                     /locus_tag="ACHK3A_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO8741862.1"
                     /translation="DIIYLTITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSLSN
                     SITNTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYYNI
                     QLRGALLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSHLAFPDNYNRLFIPR
                     QKTKDDNGLKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNKGL
                     LIAGASIVAGVVLGRMRFPDLESFEHFGARGAVSGRPFKPDLAGGPIENLTIDGVTIN
                     REGIAIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYCNL
                     GWETGEPNGLDGYELWNNAHTATLEDFKIKDTDLFHPDAKK"
     gene            1125..1382
                     /locus_tag="ACHK3A_003837"
     CDS             1125..1382
                     /locus_tag="ACHK3A_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO8741863.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            1457..1702
                     /locus_tag="ACHK3A_003838"
     CDS             1457..1702
                     /locus_tag="ACHK3A_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO8741864.1"
                     /translation="MITKNELNVLNVMTEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVSEKGYNLVQRENK"
     gene            complement(1871..4132)
                     /gene="metE"
                     /locus_tag="ACHK3A_003839"
     CDS             complement(1871..4132)
                     /gene="metE"
                     /locus_tag="ACHK3A_003839"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO8741865.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4250..5203
                     /gene="metR"
                     /locus_tag="ACHK3A_003840"
     CDS             4250..5203
                     /gene="metR"
                     /locus_tag="ACHK3A_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO8741866.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5091..5990)
                     /gene="bioP"
                     /locus_tag="ACHK3A_003841"
     CDS             complement(5091..5990)
                     /gene="bioP"
                     /locus_tag="ACHK3A_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO8741867.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(6066..6866)
                     /gene="yigL"
                     /locus_tag="ACHK3A_003842"
     CDS             complement(6066..6866)
                     /gene="yigL"
                     /locus_tag="ACHK3A_003842"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO8741868.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(6874..7896)
                     /gene="pldB"
                     /locus_tag="ACHK3A_003843"
     CDS             complement(6874..7896)
                     /gene="pldB"
                     /locus_tag="ACHK3A_003843"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO8741869.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVIVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8007..8627
                     /gene="rhtB"
                     /locus_tag="ACHK3A_003844"
     CDS             8007..8627
                     /gene="rhtB"
                     /locus_tag="ACHK3A_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO8741870.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKPL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8689..9309)
                     /gene="rhtC"
                     /locus_tag="ACHK3A_003845"
     CDS             complement(8689..9309)
                     /gene="rhtC"
                     /locus_tag="ACHK3A_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO8741871.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9373..11202)
                     /gene="recQ"
                     /locus_tag="ACHK3A_003846"
     CDS             complement(9373..11202)
                     /gene="recQ"
                     /locus_tag="ACHK3A_003846"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO8741872.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENDVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPISASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11334..12203)
                     /gene="pldA"
                     /locus_tag="ACHK3A_003847"
     CDS             complement(11334..12203)
                     /gene="pldA"
                     /locus_tag="ACHK3A_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO8741873.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITRHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12368..12835
                     /gene="yigI"
                     /locus_tag="ACHK3A_003848"
     CDS             12368..12835
                     /gene="yigI"
                     /locus_tag="ACHK3A_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO8741874.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            12887..13777
                     /gene="rarD"
                     /locus_tag="ACHK3A_003849"
     CDS             12887..13777
                     /gene="rarD"
                     /locus_tag="ACHK3A_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO8741875.1"
                     /translation="MDTKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPKKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTAK"
BASE COUNT         3474 a         3601 c         3444 g         3330 t
ORIGIN      
        1 gatattattt acctgaccat aaccggagaa caacaaggat gtatttcctc tcgttgcgga
       61 acgagcgcat caataggtaa tcgctggcag attggccatg aagatgaaat cttcgcgttc
      121 tcgctatcta atagtattac gaatacaggc aaagggtcac aattacatgg ccttagtttc
      181 tgtaagttga ttgacaaaag ctccccgtta cttattaacg ctattaacaa taacgagcag
      241 ttgttcatgg agtttgactt ctatcggatc aatagatttg gtcgatggga gaaatattac
      301 aacattcaac tccgcggcgc gctcttatca gcaatcaatc acctgtttac tgagaacaat
      361 ctggatacag aaaccatcac tgtcagttat gaatatatac tctccaggca tctgattgct
      421 aatactgaat ttagtcattt ggcatttccc gataactaca accgtctgtt tataccacgg
      481 caaaaaacta aagatgataa tggcctcaaa acactcaata gtaaagcggt tggcagactg
      541 cttgctgctg gcggcatata caatgggaat atcgaaggat tcagggaaac agcaaacaag
      601 ttgggaggag atgcacctgc tggttacgat caagttatgg ataacaaagg gttactcatc
      661 gctggtgctt caatagttgc tggagttgtt ctgggaagaa tgagattccc tgatttggaa
      721 tcatttgagc atttcggagc cagaggagca gtatcaggaa gaccatttaa gcccgacctt
      781 gccggtggtc caatagaaaa cctgacgata gatggcgtaa caatcaatag ggaaggtatc
      841 gctattgttg aaaagcatat ctccagattt gatcatgatc cagctaatga tgtaatgatt
      901 tcaaggctaa aaaaaatagc aaataaagaa ttgcttcctg aaaaatacga tctgaactac
      961 tatactcatg aatgccgcga gtatcagcgt tattgtaatt taggatggga aacaggtgaa
     1021 ccgaatggtt tagatggata tgaattatgg aataatgcac atactgcaac tctagaagac
     1081 tttaaaatta aagatactga cttatttcat ccggatgcaa aaaaatgacg attacgatag
     1141 ttgaatttaa cgtgctgaaa gttatggctg agaaagatat tgactggagc tggatggtgc
     1201 tggatcgtac tttagctata cgaaatattc caggatttgg caacgtagcg aatatcgtaa
     1261 caaagctggt taatcatggg ttggttgata tcgtaaatgg tgagggcaac tctaaaccac
     1321 gctacagagt ttctcaatat ggattgaatc ttatcaaaga acaacaagac aacctcttct
     1381 gatccagcca ttacaggagc atgatttaca ttgtgatctt tcaataagat actcatcaat
     1441 tttggtacag aatatcgtga tcactaaaaa tgaacttaac gtgctgaacg ttatgactga
     1501 gaaagatata aactggaact ggatgaatct cgatcgcaca ctatccataa ggcaaatacc
     1561 aggattcggt aatgttgtta atattgttaa caaattagct aacgaaggtt tggttattat
     1621 tgaggatagc gggcataagt ctatgcctca ctaccacgtt agtgaaaagg gatacaatct
     1681 cgtgcaacgt gagaataaat aacgtagcgt tgattactga acggaatcac tcaaaactgt
     1741 gttttgcgaa aaaaacgatt cggttatttc tactcctgaa gatctgcttc aatctggcac
     1801 tcatgcaaaa tggcttccag tactggaagc cattatcgta ctgcaattta caatgactcg
     1861 ttaatactca tcatccccga cgcaaattct gcgccgcctg caccatgttc gccagtgccg
     1921 cgcgggtttc tggccagccg cgcgttttca ggccacagtc cgggtttacc cacagacgct
     1981 ctgccggaat gcgttttgcc gctttcttca gcagggcttc aatccattcc acgctcggta
     2041 cgtttggcga gtgaatgtca tagacgcctg gaccaatttc atttggataa tcaaactctt
     2101 caaacgactc cagcaactcc atgtcggaac gcgaggtttc gatggtgatg acgtctgcgt
     2161 ccagcgccgc aatcgaatcc atgatgtcgt tgaactcgca gtaacacatg tgagtgtgga
     2221 tttgtgtgtc atctttcgcc acggcggcgt tgatacggaa agcctccacg ccccactgga
     2281 gataggcatc ccagtcgctg cgacgcagcg gtaaaccttc gcgcagtgcc ggttcatcaa
     2341 tctggatgat gccaattcca gcggcttcca ggtccgccac ttcatcacgc agcgccagcg
     2401 caatttgttt ggcgatggtt tcacggctga catcttcacg cgggaatgac cagcagagta
     2461 tggttaccgg ccccgtcagc atccctttca ccggtttgtc ggtcagcgat tgtgcatatt
     2521 tcgcccactc cacggtaatt ggtgccgggc ggctaacgtc accaatgaca atcggtggct
     2581 tcacgcagcg ggaaccgtag ctctgtaccc aaccgttttg cgtaaagaca aagccatcca
     2641 gatgctcgcc aaagtattcc accatgtcat tacgctcggc ctcgccatgt accagcacat
     2701 ccagtcccaa acgttcctgc tcgacaatgg cctgcttgat atgttccgca atgcccgtgc
     2761 ggtagttgtt ggcgtcgaga ttaccctttt tgaaatccag acgcagtgta cgaatttccg
     2821 tggtttgcgg gaaggaacca atcgtggtag tcggccacgc gggcagttta aaacgcgcac
     2881 gctgggcttc agcacgcact tcatagacat tcgcacgctg actgtcctgg gcggtaatgg
     2941 ctgcaagacg cttttctacc gccggattat ggacacgggt agagtgacga cgcgcctgaa
     3001 tcggagcgct ccattctgcc agagccgccg tatcaccact gttcagcgca tcgcgcagca
     3061 gtgccagttc atggcatttt tgtagggcga aggcaaacca gcttttcact tctgcatcaa
     3121 gacgcgtttc cacgctcagg tcgatggggc tgtgcagcaa cgagcaggaa gatgccaccc
     3181 acaaatcgcg tttgccgaca atgtccttaa tttgcgcata tttctcggta agatcggcgc
     3241 gccagacgtt acgaccattg atcagacccg cagacagtag ccagtcagaa ggcagacgct
     3301 tgtgcagttc agcaacgtca tctttaccat gtacaaggtc cacatgcagc ccctgaacag
     3361 gcagcgcagt aatcgtgtcg agatttggcg ttacgccttc aaaataggta gtcagcagca
     3421 gtttcacctg gccctggagc gcgtcgtaag cgggtttgta tgcgtccagc cacgcctgcg
     3481 gtagttccag taccagcgct ggttcgtcga tctgtaccca ctcaatgccg cgttttgcca
     3541 gttctgccaa cacttgctga taaaccggca gaatgtcgtt cagcaggctc aggcgatcaa
     3601 actgttcacc tttcacttta cccagccaca ggtaggtaac cggccccagc agcacgggct
     3661 tgaccttgtg gcccagcgcc agcgcctcgt ccacttcctc cagcagctgc gtccaggtca
     3721 gtttgaactg ttggccttta acgaactccg gcaccatgta gtgatagttg gtgttaaacc
     3781 atttggtcat ttccgctgcc gccgcaggtt cgccagtcgg tgcacgtcca cgaccaatac
     3841 ggaacagggt gtcgatatct accgaaccat ctttgttctg atgacgtggc ggaacgttac
     3901 ccagcagcag actggtggtc agtacatgat cgtaccaggc aaaatcgccc accggcagca
     3961 ggtcgatacc cgcctgcttt tgttgatccc agtgacgggc acgtaattca cgcccaacag
     4021 ccagcagttc ttcacgcgtg gagttccccg cccaataact ctcttgcgct tttttcagct
     4081 cgcgacgcag gccaacgcga gggaaaccga gggtgtgatt aagaattgtc attatatgcc
     4141 ccttatggaa tttttagtat ttagccgtcc agatgtttac acatccataa ttggcggtta
     4201 ctgtatattc ctcaagcgca aattattcat gccgaagtga aggactttca tgatcgaagt
     4261 aaaacacctg aaaacgctac aagcgttgcg gaactgcggc tcactcgcag ccgctgcggc
     4321 gacgttgcat caaacgcaat ccgccctgtc tcaccagttt agcgatctgg aacaacgcct
     4381 tggcttccgg ctatttgtgc gtaaaagcca gccgcttcgg tttacaccgc agggagaaat
     4441 cctgttgcaa ctggcgaatc aggtgctgcc tcaaatcagc caggcactgc aagcctgcaa
     4501 tgaaccgcag cagacgcgcc tgcgcattgc cattgagtgc catagctgta ttcagtggct
     4561 gacgcccgcg ttagaaaatt tccataagaa ctggccgcag gtggagatgg attttaaatc
     4621 gggcgtgacg tttgacccgc agcccgcgtt gcagcagggt gagctggatc tggtgatgac
     4681 ctccgatatt ctgccgcgca gtggcctgca ttattcgccg atgttcgatt atgaagtgcg
     4741 tctggtgttg gcacctgacc atccactggc ggcgaaaacg cgaatcacgc cggaagacct
     4801 cgccagcgaa acgctgttga tttatccggt gcagcgtagc cgactggatg tctggcggca
     4861 ttttcttcag cccgcaggcg ttagcccgtc actgaaaagc gtcgataaca ccttgttgtt
     4921 gattcagatg gttgccgcgc ggatgggcat cgctgcgcta ccgcattggg tggtggagag
     4981 ttttgaacgc cagggtctgg tggtcaccaa aaccctgggc gaaggcttgt ggagccgact
     5041 gtacgccgcc gtgcgcgatg gcgagcagcg tcagccgatt acagaagcgt ttattcgttc
     5101 agcgcgcaat cacgcctgcg atcatctgcc gtttgtgaag agcgcggagc gacccactta
     5161 cgatgcaccc acagtgaggc caggatcacc agcgcgcctg taataaacgt tggccagtgc
     5221 ggctgttggt gccagatagc caggttaacc aacagccctg ccggaacgtg catattattc
     5281 ataatgccca gcgttccggc gtccacctga gtcgcgccgt agttccacat aaagtagcca
     5341 ataccggaag ccaccacgcc aagaaacacc agaatgcccc attgcagcgt ggtttgcggc
     5401 attttctgcg catttcccag caaaaaccat gcaatcaccg ccaccaggaa cgcaccaaga
     5461 tagaaccacg caaaggcgtt atgctgtggc atcggacgag tttccatcag gcgtttgtaa
     5521 cccaccatgc cgatggcaaa agtgatattg gagagttgca ccagcagcag gccagtccag
     5581 aagtggtcgg tgacctgatc atagcgaata attccggcac caatcaccgc caacaaggcg
     5641 ctaaaagcat agccccagcg cagacggcgc ttactcatga tgtcataaat cagcgtgatg
     5701 tagagcggag tcagtacggt gaacagcaac agttcggaga ccgtcaggta gagataggcg
     5761 cgaaaactca gcatatacat cacaccgagc tgcatcgcgc ccaccagcat atacaggcca
     5821 accgttttca ggctattgcc acgggtacgc agaaacggca gaaacacgag tgccgccagg
     5881 ccaacgcgca ccagcactgc aaaatagcta tcgacgtgcc ccgcaaggta ctcgccataa
     5941 aagctaaagg agaaggccca cagaatcgtg gtgatgataa gtagcgccac aatggaaaac
     6001 tctttgatta acgagtatcc attgtagcga agtatcaggt tgacaactga ccaaataaag
     6061 aacgattacg ataaatagag tttacgcaga taatgcggca ccgcgtcttc ggcattagta
     6121 ccaatcactt ccagctccgg atgcagatct ttcagacgct ggtgcgcact gcccataatg
     6181 cagcctttac ctgccatcga cagcatttcg gcgtcgttca tcccgtcacc aaaggcaata
     6241 caatctttca gactgtagcc cagtttcttc gccaccgctt ccagggcatg gccttttgaa
     6301 acgccgcccg ccatcacttc cagacaagtt agggtagaga agctgacgtt gacgcgatcg
     6361 ccccaacgag cgttgattgc ctgctccagc ggcagcagtt gctcatgaga atcgcaggtg
     6421 aagaacactt tgctgacgcc ttccggctcc agtaatcctg gctcatacag cgcatatttg
     6481 aacactgctt ctttaaaaaa gcgcatctct tccgggcggt ggcgattcat aaaccattcg
     6541 tcgtcgcgat aaacgttagt aatgatgtcc gggttgtcgt tgaccacgcc aaacagatcg
     6601 ctggcaatgt cgcgatccag gttatgagca aaaatcagat tgccatccag atcgtgaaca
     6661 cgcgcgccat tggaggtaat catgtaagac ttaatctcca gattatcgcg aatttgccct
     6721 acatcaacgt ggtggcgacc ggtcgcaaac acaaagttaa tgccgcgcgc ggtgagcagc
     6781 ttcagagttt ctttggcgta aggggataac gtatggtcgg gagaaagtaa cgtgccatct
     6841 aaatcagacg caacaacctg gtacataaga aatttaaacc tctgtagagc ggtttccgct
     6901 gggtgagtta tgcctgttga aaaaatcaac aatggcgtgg agcgcgactg agcgcattgc
     6961 gtccttttca aaaaggatct catggtacgc acctttaatt accagcggtc gtcccccttc
     7021 gacaggatgg cccgcagcgg tgcggagttc acaaaaacgg tcgtgcatgc ggttatccac
     7081 cacgcgttcc tcttcagcct gcaagagaag cgttggcgtg gcgtcatcac ccgcaccggc
     7141 taacacctgt tcgccagcca gaatactttc gcgtacccaa tggtaggtcg gcccaccgac
     7201 gcgaatcgtt gggtcatcag cataaaagcg taagttacgt cgatatcgct gtctgctgtg
     7261 ggtcagtacg ttgatagcaa acggcaacgc gcgccagcgc ccggtgccta ttgcatagcc
     7321 atcacggaaa cgtggatgcg cttcggccca gttaaggatc tgccgcgcca taaatgacgg
     7381 catacgaatc acgatcccaa acattggcgc agttagcgca atggcgtcac atacacctgg
     7441 atggcgttgc agaaataacg tggagatcgc accgcccatc gaatgtgcca gtatatagcg
     7501 cttacgccac ggaccaggct ggacctcctg ttgccagaat gccgccaggt catcgacata
     7561 atcattaaag cgattaacat gcccaagatg cggatcggct aacaggcgac cggaacgtcc
     7621 ctgcccgcga tggtcgatga ttaagacatc aaaccccaaa tggaacaggt cgtaggccag
     7681 ttccgcatat tttacgtagc tctcaatacg ccccgggcag atgactatca cccggtcatg
     7741 gtgctgtgcg cgaaaacgga caaagcgcac cggaatgtca tctacaccag taaactctgc
     7801 ttcatcacgc tgacgccaga aatcagtcag cggtcccatg gtaaaagcag caaacgcgtt
     7861 ttctcttgtt tcccagtctt tttgctgctg aaacatcggg taatctgcct cttaaaccac
     7921 gtaaaatcgt ttttttaagc gtgcctgaca caacgcttcg acagtagcgt attgtggcac
     7981 aaaaatagac acaccgggag ttcatcatga ccttagaatg gtggtttgcc tacctgctga
     8041 catcgatcat tttaagcctg tcgccaggct ctggtgcaat caacaccatg accacctcgc
     8101 tcaaccacgg ttatcgcggc gcggtggcgt ctattgctgg gcttcagacc ggactggcga
     8161 ttcatattgt gctggttggc gtggggttgg ggacgctatt ttcccgctca gtgattgcgt
     8221 ttgaagtgtt gaagtgggca ggcgcagctt acttgatttg gcttgggatc cagcagtggc
     8281 gcgccgctgg cgcaattgat cttaaaccgc tggcctctac tcaatcgcgt cgacatttgt
     8341 tccagcgcgc agtttttgtc aatctcacca atcccaaaag tattgtgttt ctggcggcgc
     8401 tatttccgca attcatcatg ccgcagcagc cacaactgat gcagtatatc gtgctcggcg
     8461 tcaccactat tgtggtcgat attattgtga tgatcggtta cgccacactt gctcaacgga
     8521 ttgcgttatg gataaaaggg ccgaaacaga tgaaggcatt gaataagatt tttggctcgc
     8581 tgtttatgct ggtaggagcg ttgttggcat cggcgaggca tgcgtgaaaa ataatgtcgg
     8641 atgcggcata aacgccttat ccgacttact ctgatgaagc gtttggcatc aacgagaaat
     8701 aatcaaatga atgccaaatc cggcaaataa cgccccggca aaaccatcaa tccacttcgc
     8761 cagacgttga taaccacggc gcatttgcgg tagagcaaac aggctggcaa ccaccgtaaa
     8821 ccatgccagt gtttcgataa taatcaacgc gaaaatgccc cagcgctcag tggtgccgac
     8881 gttatcgccg acaaacagcg agaataccga accaaagtag ataattgctt tcggattggc
     8941 gagattggtc agtaagcctt tcaggaaact gcgcccgctt tttgccagtt ctacctgtgg
     9001 cgcaggcgca gaaacgacct cttttttcag tgcgctacgc agcatctgat aacccatcca
     9061 gcagagatac aggccaccgc ccaccataat cagcgtatgc agccaggcca ttttttcgat
     9121 aatcaaatgc aggccaagca acgcgatccc tgcccagacc attacgccgc aggtaatgcc
     9181 cagcacgccc atcatcgcct ctttacggga acgactgaca gcggtctgag aaacaaagaa
     9241 gaaatcggga ccggggctca taagcgccac aatgtgcacc atggcaacgg tgaggaataa
     9301 catcaacatt caattgactc gcggagaaat cgtttacgga gtcttcatcc tggcactttt
     9361 ttatgctgct gactactctt cgtcatcgcc atcgacatgc gcacgaatta gcgccataaa
     9421 cggtttgcca aagcgttcca gcttgcgcat cccaacgccg ttaacgctga gcatttcgct
     9481 ggcggagatc ggcatctgtt ccgccatctc aatcaaggtc gcatcgttaa acaccacata
     9541 cggcgggaca ttactttcat cggcaatcga tttacgcagt ttgcgtaatt tggcgaacag
     9601 tttgcgatcg taattgccac cgaaagattt ctgcattgct ttcggtttga gcgccacaat
     9661 acgcggcacg gcaagttgta aagaggattc gccgcgcaac accgggcgtg cggcttctgt
     9721 cagttgtaat gccgaatgct gggcgatgtt ttgcgtcacc agaccgagat gaatcaactg
     9781 gcggattacg ctcacccagt gctcatgact tttatcgcgg cccatgccgt agactttcag
     9841 tttgtcatga ccatagtcgc ggattcgctg gttattggcg ccgcgaatca cttccactac
     9901 atagcccatc ccaaaacgct gattcacacg accaatggtg gaaagcgcaa tctgcgcatc
     9961 ggttgaacca tcgtattgtt tcggcggatc gaggcagata tcgcagttcc cgcacggctc
    10021 ctgccgccct tcgccaaagt agttcagcag caccagacga cggcaagttt gcgcttcggc
    10081 aaaagcgccc atcgcattga gtttatggcg ctcgatatcc tgcaactgcc cctgcggctt
    10141 ctcttccaga caacggcgca gccacgccat atcagccgga tcgtaaaaca gcatcgcttc
    10201 cgcgggcagg ccatcacgcc cggcgcgacc ggtttcctga taataggatt cgatattgcg
    10261 tggaatatca aagtgaacca cgaagcggac gttaggcttg ttgatgccca tgccgaacgc
    10321 taccgtcgcc accacaattt gcaggtcatc acgctggaat ttttcctgca cgtccgcacg
    10381 gacatcgttt tcaagcccgg cgtgataagc cgctgcgctg atacccttgc tttgcagacg
    10441 cgcggcggtg tcttctactt tcgcacgact attacagtaa ataatccccg acttaccgcg
    10501 ttgttcctgt acataacgca tcaactgatc gagcggtttg aatttctcca tcagcatgta
    10561 gcgaatattc ggacggtcaa aactgctaat ttgaatcagc ggatcgttca gccccagcag
    10621 gcgcacgata tcctgccgcg tggtgtcgtc ggcggtggcg gttagcgcca taaacggcag
    10681 cgtcgggaac cgctggcgca actgaccgag cgcggcatat tccgggcgga aatcgtggcc
    10741 ccattgggag atacagtgcg cttcatcaac ggctaataac accggattcc agtgcgccag
    10801 atgctcaaga aagttatcca gcatcaggcg ttccggggcg atataaagca gacgaatttg
    10861 cccggtgcga cagcctgtca tcacttcaag ctgctgctcg cgggtttgcg tcgagttaag
    10921 gcacgccgcc gccacaccgt tggcctgtag ctgatccacc tgatctttca tcaacgaaat
    10981 cagtggtgaa acaaccacgg taagaccgtt tagcagtaag gcaggaattt gatagcaaag
    11041 ggattttccg ccaccagtgg gcatgacgac caggcaatcg cgaccggaaa gtacggtgtc
    11101 gataatctct tcctggccgg ggcgaaactg ttggtagcca aaggtttctt gtaaaacctg
    11161 tttagctccg gactccagat tcaacacttc cgcctgcgcc acattaaccc cgattgcctg
    11221 aaataaaaac aggcgctatt ttcagcgcct gcgcgagaaa ctgcaatgct tttagtctct
    11281 gagagggtaa tcctggatac tggattccag ttttgccttt caatcaactc acattagaac
    11341 aagtcgttca gcataacccc cacacccaca cgggtctggt taaagttata gtcgatgagc
    11401 gattcgccat aaccgctgta aacctgggta taaaggcgca catgcctggt gatcgggtaa
    11461 cttaagccta actccgcacc accgtaaccg gtgttccagt tgtactgtcc tttcgcactc
    11521 agcaccgcat cacccaggtg atagccgatt ttaagctggt agtaacccat atatttggtg
    11581 atatctgggt tatcgtcagt attccccacc acataccacg gcttcacctc taccagccag
    11641 ttaccgtttt ctgccatcag acgggtataa aggcggttcc agctgcggga agtcgggtct
    11701 gaacgcccgt tagagtcgtg gttataaccc atctccacat cgcgcagcgt ccagccagca
    11761 aagcggtaat cggtggcaaa accaaggaac aattgcggtt cgtagttggt ttcacgaaac
    11821 ggtgaagact cttcgctatt ggacagttgc caccaggatt tttgcgtgta agaagccccc
    11881 aacaccgagt ttggccccaa tattccgcgc cacagcggaa atgccaggct caactggaat
    11941 ttcacttcat ccttacgcgc gttttccgcc cagtcataac tggcgatcgc ttctttgttc
    12001 agatcgctgg tttgggtgta gatgaggtag ttggtgtcat aaggatagag cgtgaaagga
    12061 ttgtcatgct cctgcagcat attcgcaata atactgccac gcaccgcagg cgcgtcatgc
    12121 acctctttta ccgttgcctc ttgcgcatac actgccattg gcaacataaa caccggcaac
    12181 aaccagccct gcagagtccg catcggtcgt gttctccgta acgaattatt gattagatga
    12241 atatttttct gcgggctatt ttacatactt aactcctttg ccgttagtta tcccttccta
    12301 aagtagaaaa ccacaattaa gaagcataaa atcaacattt cattaaccaa tgaggtgtaa
    12361 ggatgatatg tctgccgtac tgaccgctga acaagccctg aaattagtgg gtgagatgtt
    12421 tgtttatcac atgccattta accgcgcatt ggggatggaa ctggagcgtt acgaaaaaga
    12481 gttcgcacag ctggccttta aaaatcagcc aatgatggtg ggtaactggg cgcaaagcat
    12541 tttgcacggc ggggtcattg cgtcggcgct ggatgtcgcc gcaggtctgg tgtgcgtggg
    12601 aagtacctta acccgccacg aaaccatcag tgaagatgaa ctacgccagc ggctatcgcg
    12661 gatggggacc attgatcttc gcgtcgatta tctgcgccca ggcaggggcg agcgttttac
    12721 tgctaccagt agtctgttgc gtgcaggcaa taaagtcgcc gttgcccgcg ttgaattaca
    12781 caatgaagaa cagctttata tagccagtgc caccgccacc tatatggtag gttgataacg
    12841 taaaagcagg taaactgctt ttaccaataa aaaatgagtt ttgccaatgg acaccaaaca
    12901 aacgcggcag ggcgtattac tcgctcttgc cgcttatttt atttggggta ttgcgccagc
    12961 gtacttcaag ctgatttact acgtgcccgc cgatgagatc ctgacgcatc gcgtgatctg
    13021 gtcgtttttc tttatggtgg tgctgatgag catttgccgc cagtggtcct atttaaaaac
    13081 gctgattcag acaccaaaga aaatcttcat gctggcagtc tctgccgtgc tgattggtgg
    13141 caactggcta ctgtttatct gggcggtgaa caatcaccat atgctggaag cgagccttgg
    13201 ttactttatt aacccgctgg tgaacattgt gctggggatg attttcctcg gcgagcgatt
    13261 ccgccggatg caatggctgg cggtgattct ggcgatatgt ggtgtattgg tccagctctg
    13321 gacttttggt tcgctgccaa tcatcgcgct ggggctggcg tttagttttg ccttctacgg
    13381 cctggtacgc aagaaaattg ccgttgaagc gcaaaccggc atgttaatcg aaaccatgtg
    13441 gctgctgccc gtggcggcga tttacctgtt tgctattgcc gacagctcaa ccagccatat
    13501 ggggcaaaac ccgatgtcgc tgaatttact gctgatcgcc gccggtattg tcaccaccgt
    13561 gccactgtta tgttttaccg cagcagctac acgcttacga ctctcaacat taggcttttt
    13621 ccagtacatt gggccgacgc taatgttcct gttggcagtg acgttttatg gtgaaaaacc
    13681 gggtgccgat aagatggtga ctttcacctt tatttgggtg gcgctggcaa tttttgtgat
    13741 ggatgcgatt tatacgcagc gtagaacggc caaataaaaa ataaatatat tctgaaaaaa
    13801 tatgccgact cttgcatgag agtgggcata ttgccatgct gacaggaga
//
LOCUS       DAZGHF010000123        13752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGHF010000123 DAZGHF010000000
VERSION     DAZGHF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.124"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            166..1224
                     /gene="citC"
                     /locus_tag="ACHK3A_003850"
     CDS             166..1224
                     /gene="citC"
                     /locus_tag="ACHK3A_003850"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO8741876.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFCHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1239..1535
                     /gene="citD"
                     /locus_tag="ACHK3A_003851"
     CDS             1239..1535
                     /gene="citD"
                     /locus_tag="ACHK3A_003851"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO8741877.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1532..2440
                     /gene="citE"
                     /locus_tag="ACHK3A_003852"
     CDS             1532..2440
                     /gene="citE"
                     /locus_tag="ACHK3A_003852"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO8741878.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2451..3983
                     /gene="citF"
                     /locus_tag="ACHK3A_003853"
     CDS             2451..3983
                     /gene="citF"
                     /locus_tag="ACHK3A_003853"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO8741879.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVYLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3987..4538
                     /gene="citX"
                     /locus_tag="ACHK3A_003854"
     CDS             3987..4538
                     /gene="citX"
                     /locus_tag="ACHK3A_003854"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO8741880.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKLGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4513..5391
                     /gene="citG"
                     /locus_tag="ACHK3A_003855"
     CDS             4513..5391
                     /gene="citG"
                     /locus_tag="ACHK3A_003855"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO8741881.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5442..6905
                     /gene="citT"
                     /locus_tag="ACHK3A_003856"
     CDS             5442..6905
                     /gene="citT"
                     /locus_tag="ACHK3A_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO8741882.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7019..7825
                     /gene="rna"
                     /locus_tag="ACHK3A_003857"
     CDS             7019..7825
                     /gene="rna"
                     /locus_tag="ACHK3A_003857"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO8741883.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETATKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISVKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8055..8465
                     /gene="rnk"
                     /locus_tag="ACHK3A_003858"
     CDS             8055..8465
                     /gene="rnk"
                     /locus_tag="ACHK3A_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO8741884.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8608..8697
                     /gene="yldA"
                     /locus_tag="ACHK3A_003859"
     CDS             8608..8697
                     /gene="yldA"
                     /locus_tag="ACHK3A_003859"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO8741885.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            8822..9250
                     /gene="uspG"
                     /locus_tag="ACHK3A_003860"
     CDS             8822..9250
                     /gene="uspG"
                     /locus_tag="ACHK3A_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO8741886.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9371..10936)
                     /gene="ahpF"
                     /locus_tag="ACHK3A_003861"
     CDS             complement(9371..10936)
                     /gene="ahpF"
                     /locus_tag="ACHK3A_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO8741887.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11107..11670)
                     /gene="ahpC"
                     /locus_tag="ACHK3A_003862"
     CDS             complement(11107..11670)
                     /gene="ahpC"
                     /locus_tag="ACHK3A_003862"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO8741888.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12042..12788
                     /gene="dsbG"
                     /locus_tag="ACHK3A_003863"
     CDS             12042..12788
                     /gene="dsbG"
                     /locus_tag="ACHK3A_003863"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO8741889.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKMLNIIMGNK"
     gene            12993..>13752
                     /locus_tag="ACHK3A_003864"
     CDS             12993..>13752
                     /locus_tag="ACHK3A_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8741890.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FINRKVSFRSSSLLTIINSISVTDLLGIVPTELYD"
BASE COUNT         3339 a         3565 c         3397 g         3451 t
ORIGIN      
        1 atttcacgct gcaatactcc ttaggatgta tagcgaaagg agaaaaagat atacctcgat
       61 cgcccccctt tctcccaagt gaaaataaaa ggttatcagt ttgcaacatc gaacaacatt
      121 tgttgcaaat cgataacaac atgcaccttc aggatactat ttattatgtt cggcaatgat
      181 attttcaccc gtgtaaaacg ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg
      241 catgaaaatg atttgagcgt tgacaccaca gtcgaagtat ttattaccgt aacccgcgat
      301 gaaaagctta tcgcgtgcgg tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt
      361 gaatccgttc gcggtgaagg actggcgctg acattagcca ccgagctaat taacctcgcc
      421 tatgagcggc acagcacgca tctatttatt tataccaaaa ccgaatacga ggcgctgttc
      481 cgccagtgcg gtttttccac gctgaccagc gtacccggcg tgatggtgct gatggaaaac
      541 agcgccacgc gactgaaacg ctatgccgaa tcgctgaaaa aattttgtca tccagggaac
      601 aagattggct gcattgtgat gaacgccaat ccctttacga atggtcaccg ttatctgatt
      661 caacaggctg cggcacagtg cgactggttg catctgtttt tagtcaaaga agattcttca
      721 cgcttcccct atgaagaccg actggatctg gtgttaaaag gcaccgccga tattccacgc
      781 ctgactgtgc atcgcggctc cgaatacatc atctcccgcg ctacgttccc ttgctacttc
      841 attaaagaac agagcgtcat taaccattgt tacaccgaaa ttgatctgaa gattttccgt
      901 cagtacctcg ctcccgcgct gggtgtaact caccgctttg tcggtactga acccttttgt
      961 cgcgttactg cccagtacaa ccaggatatg cgctactggc tggaaacacc gactatctcc
     1021 gcaccgccca tcgaactggt tgaaattgag cggctgcgtt accaggagat gccgatatcc
     1081 gcttcccggg tacgtcaact gctggcgaaa aacgatctca cggctatcgc gccgctggtc
     1141 cctgcagtca cgctgcatta tttgcagaac ctgcttgagc actcccgcca ggacgcggca
     1201 gctcgtcaaa agacccccgc atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg
     1261 ttgcaggcac ccttgagtct ggggatgtga tgatacgcat cgccccactc gatacgcagg
     1321 atatcgacct gcaaatcaac agcagcgttg agaaacagtt tggcgatgca attcgcacca
     1381 ccattctgga cgttctcgcc cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca
     1441 aaggcgcact ggactgcatt ttacgtgcac gactggaagc cctgctggca cgcgccagcg
     1501 gtatcccggc tctgccatgg gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa
     1561 aactcgcacc cgccgcagta tgttgtttgt gcctggtgct aatgccgcga tggtcagcaa
     1621 ctcctttatc tacccggctg atgccctgat gtttgacctc gaagactccg tagcattgcg
     1681 tgaaaaagac accgcccgcc gcatggttta ccacgcgctg caacatccgc tttatcgcga
     1741 tattgaaacc attgtgcgtg taaacgcgct ggattccgaa tggggtgtta acgacctgga
     1801 agctgtcgtt cgcggtggtg cggacgttgt gcgtctgccg aaaaccgata ccgctcagga
     1861 tgttctggat atcgaaaaag agatcctgcg tatcgaaaaa gcctgtggtc gtgaacccgg
     1921 cagtaccggc ctgctggcgg cgattgaatc tccgctgggc attacccgcg cggtagaaat
     1981 cgctcacgct tccgagcgtt tgatcggtat cgccctcggt gcagaagact atgtgcgcaa
     2041 cctgcgtaca gaacgctccc cggaaggaac tgaactgctg ttcgctcgct gctccatttt
     2101 gcaggccgcg cgctctgcgg gtattcaggc gttcgatacc gtctattccg acgctaacaa
     2161 cgaagccgga ttcctgcaag aagccgccca catcaaacag ctgggctttg acggcaaatc
     2221 gctgatcaac ccgcgtcaga ttgatctgct gcacaacctc tacgcaccga cccagaaaga
     2281 agtggatcac gcccgccgcg tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg
     2341 cgtggtttcc ctgaacggca agatggtgga cggtccggtt atcgatcgcg cccgtctggt
     2401 gctctcccgt gcagaacttt ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga
     2461 aaattgaaca atctcaacga caagaacggg tagcggcctg gaatcgtcgc gctgaatgcg
     2521 atcttgccgc tttccagaac tcgccaaagc aaacctacca ggctgaaaaa gcgcgcgatc
     2581 gcaaactgtg cgccaacctg gaagaagcga ttcgtcgctc tggtttacag gacggcatga
     2641 cggtttcctt ccatcacgct ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg
     2701 tcatcgcgaa gatgggcttt aaaaacctga ctctggcctc cagctccctg agtgattgcc
     2761 atgcgccgct ggtagaacac attcgccagg gcgtggttac ccgcatttat acctctggcc
     2821 tgcgcggtcc actggcggaa gagatctccc gtggtctgct ggcggaaccg gtgcaaatcc
     2881 actctcacgg cggacgtgtg catctggtac agagcggcga actgaatatc gatgtggctt
     2941 tcctcggcgt cccgtcctgt gatgaattcg gtaatgccaa cggctactcc ggtaaagcct
     3001 gctgcggctc cctcggctat gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc
     3061 ttaccgaaga actgctgcct tatccgcata atccggcaag cattgagcaa gatcaggttg
     3121 atctgatcgt caaagttgat cgcgttggcg atgctgcaaa aatcggcgct ggtgctaccc
     3181 gtatgaccac taacccgcgc gaactgctta ttgcccgtag cgctgcggat gtgattgtta
     3241 actctggcta cttcaaagaa ggtttctcca tgcagaccgg caccggcggc gcttcgctgg
     3301 cggtaacccg tttcctggaa gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc
     3361 tcggcggtat taccgcaacg atggtttatc tgcacgaaaa aggtttgatc cgcaaactgc
     3421 tggatgtgca gagctttgac agccatgctg cgcaatcgct ggcccgtaac cccaatcaca
     3481 tcgaaatcag cgccaaccag tacgctaact gggggtcgaa aggcgcagcg gttgatcgtc
     3541 tcgacgtggt ggtactgagc gcgctggaaa ttgacaccca gttcaacgtt aacgtgctga
     3601 ccggctctga cggcgtactg cgtggtgctt ccggtggtca ctgcgatacc gcgattgctt
     3661 ctgcgctttc catcatcgtc gcgccgctgg tacgcggtcg tattccgacg ctggtggata
     3721 acgtgctgac ctgcatcacc ccgggctcca gtgtcgatat tctggtcaca gaccacggca
     3781 tcgctgttaa cccggcacgt ccggagctgg cagaacgtct gcaggaagcg ggcattaaag
     3841 tggtttccat tgagtggctg cgtgaacgtg cgcgtctgct gaccggtgaa ccacagccga
     3901 ttgaattcac cgaccgcgtc gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg
     3961 ttgtgcatca ggtgaaggaa taagccatgc acctgcttcc tgaactcgcc agccaccatg
     4021 cggtgtcaat tcccgagctg ctcgtcagcc gggatgaaag gcaagcacgg caacacgtct
     4081 ggctcaagcg ccatcctgtt ccactggtct cctttaccgt ggttgcgcct gggccgatta
     4141 aagacagcga ggtcacacgc cgaattttta atcatggcgt gacagccttg cgtgccttag
     4201 ccgcaaaact gggctggcaa attcaggagc aggctgcact ggtttccgcc agcgggccgg
     4261 agggcatgtt gagcattgcc gccccggctc gcgacctcaa gctcgccacc attgagcttg
     4321 aacatagtca tcctctcggg cggttatggg atatcgatgt cctgacaccc gaaggcgaaa
     4381 ttctctcccg ccgcgactat tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg
     4441 cagctgtttg cgcgcgtgga aaaacccatc aactgaccga tttactcaac cgcatggagg
     4501 cgctgctgaa cgatgtcgat gcctgcaacg tcaactaaaa acacaaagct tgcgacgtca
     4561 ttaatcgatg agtacgccct gctgggctgg cgcgccatgc tgactgaagt caatctgtca
     4621 ccgaaaccag gcctcgtgga tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa
     4681 gatttccacc gcagcgcgct ggcgattcag ggctggttac cccgtttcat tgaatttggt
     4741 gcctgtagtg cagaaatggc accggaagcg gtactcaacg gattacgccc aattggtatg
     4801 gcttgcgaag gtgatatgtt ccgcgccact gcgggcgtaa acacgcataa aggaagcatt
     4861 ttttctttag ggctactgtg tgcggcaatt ggtcgtttgc ttcagctcaa ccagccggta
     4921 acgccaacaa ccgtttgttc tacggcggca agtttctgcc gtggcctgac cgatcgcgaa
     4981 ctgcgtacca ataattcacg actgacggca ggtcaacggt tgtaccagca gcttggcctt
     5041 accggcgcac gcggtgaagc cgaagcgggt tatccactgg tgatcaacca cgccttgccg
     5101 cattacctca ctctgctgga tcaggggtta gatcctgagc tggcattgct cgataccttg
     5161 ctcctactga tggctaccaa cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc
     5221 ctgcgctggc tacagcgcga ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc
     5281 gccgatctcg attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca
     5341 ggcggcagtg ccgacctgct gatccttacc tggtttttag cacagattta attatttaag
     5401 cacttgataa atttggaaat attaattttc ggagaacccg tatgtcttta gcaaaagata
     5461 atatatggaa attattggcg cccctggtgg tgatgggtgt catgtttctt atccctgtac
     5521 ctgacggtat gccgccgcag gcgtggcatt actttgcagt gtttgtggca atgattgtcg
     5581 gcatgatcct cgagccaatt ccggcaacgg cgatcagttt tattgcggtt actatttgcg
     5641 ttattggcag taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg
     5701 cgcaaaaaca ggcgctgaaa tggggtctgg ctggtttttc cagcaccacg gtatggctgg
     5761 tatttggcgc atttattttt gcattagggt atgaagtttc cgggttaggc cgtcgtattg
     5821 cccttttcct ggtgaaattc atgggcaaac gcacgctgac gttgggttat gcgattgtca
     5881 ttatcgacat tctgctggca ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg
     5941 tttttccggt cattaaaaac ctgccgccgt tgtttaaatc attcccgaac gatccgtccg
     6001 cgcgtcgtat tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt
     6061 ccatgtttgt caccggtgcg gcaccaaacg tgctgggtct ggagttcgtc agcaaaattg
     6121 ccggtatcca gattagctgg ttgcagtggt tcctctgctt cctgccggtt ggggttatct
     6181 tgcttatcat tgcgccgtgg ctttcctacg tgctgtacaa accggaaatc acacacagtg
     6241 aagaagtggc aacctgggcg ggtgatgaac taaaaaccat gggtgcgctg acacgcagag
     6301 agtggacgct gattggcctt gtattgctca gcttaggttt gtgggtattt ggcagtgaag
     6361 tcattaatgc tactgcggtt ggtctgctgg cagtttcgct aatgctggcc ctgcacgttg
     6421 tgccgtggaa agacattacc cgctataaca gcgcatggaa cacgctggtc aacctggcaa
     6481 ctctggttgt gatggctaac ggcctgactc gttctggttt tattgactgg ttcgccaata
     6541 ccatgagtac gcacctggaa ggattctcac caaacgcaac ggtgattgta ctggttctgg
     6601 tgttctactt tgcacactac ctgtttgcca gcctgtctgc gcacaccgca accatgctgc
     6661 cggttattct ggccgtcggt aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc
     6721 tgctggtgct atctatcggt atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg
     6781 tgattattta cggctgtggc tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct
     6841 tcggggtgat ttacatctct atgttgctgt tggttggctg gccgattctc gccatgtgga
     6901 actaatacgt tcgccaaacg ctgaataaat catcacgccc gccaggtaaa cccacctggc
     6961 gggctttttt atgattaaat agataatatt cagtgtatgt atgagttcca cacccattat
     7021 gaaagcattc tggcgtaacg ccgcgttgct cgcggtttct ctgcttccct tctcttctgc
     7081 caacgccgtg gcgttgcagg caaaacagta tggcgatttt gatcgctatg tcctggccct
     7141 ctcctggcaa accggatttt gccagagtca acacgatcga aatcgtaacg aacgagatga
     7201 atgtcgcctg caaaccgaaa cgaccaacaa agctgatttt ctgaccgtac atggtctgtg
     7261 gccaggattg cctaaatcgg ttgctgcccg tggtgttgat gaacgccgct ggatgcgctt
     7321 cggttgcgct actcgcccaa tcccgaatct accagaagcg cgcgccagcc gaatgtgttc
     7381 atcgccggaa accgggttat cactggaaac ggccactaaa ctaagtgaag tcatgccagg
     7441 agctggcgga cgttcctgcc tggaacgcta cgaatatgcc aaacacggtg cctgctttgg
     7501 ttttgatcca gacgcatact tcggcacgat ggtacgcctg aatcaagaaa ttaaagaaag
     7561 cgaagccgga aaattccttg cggataacta cggtaaaaca gtgagccgcc gtgactttga
     7621 tgccgccttt gccaaaagct ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca
     7681 gggtaaccct gcgtatttga ctgaaattca gatctcggtc aaagctgacg ccatcaacgc
     7741 tccgctttct gccaactcat tcttgccaca accacaccca ggcaactgtg gcaaaacctt
     7801 tgtgattgat aaagcaggtt attaatcctc tgatttacaa aacacgctaa ttcgtcttcc
     7861 ataaaccatg acgattaaga tttatacata atgcgctaca ttgaggaatt ccctgaatgt
     7921 gacgcaaatc acttcaaaga gtgagcaaca gtcagtatca tcatgtgagt taaaccctcg
     7981 ccgcctgacg gtgagggttt tcttttgggt atgtttatct gcgacactcg cagtaccgac
     8041 gacatggagt aaaaatgtcc agaccaacta tcatcattaa cgacctggat gccgaacgca
     8101 tcgatattct gctggagcaa cccgcctatg ctggtttgcc aatcgccgac gcgttaaacg
     8161 cagagttgga tcgcgcccaa atgtgttcgc cagaagagat gccacacgac gtggtgacaa
     8221 tgaacagccg ggttaaattc cgcaatctta gcgatggcga agtgcgtgtg cgcacgctgg
     8281 tgtatccggc aaaaatgacc gacagcaata ctcagctttc cgttatggct ccggtaggtg
     8341 ccgcactgct ggggctgcgc gttggcgatt ccattcactg ggaacttccg ggcggcgttg
     8401 caacccacct tgaagtgctg gaactcgaat accagccaga agctgctggc gactacctgc
     8461 tttaatccca tctgcacaag cgataactcg ctttgcccag aggatgcact cgcatcctct
     8521 ttccgctcaa atatcaacca gatttatcat tttgctctta agcgcagcat tctaatctga
     8581 actggctatt catctgcgga acctcatatg agcgaagctc tcggtatcac tgcattttat
     8641 atattcatag cagcaattat tgttgctgca gtgctatacc ttgaacaacg ctggtagcgc
     8701 ctggttttta tttattttcc agctcaatag caaattcaga tcaaatcagc ctcgcaaatt
     8761 atagattcgt aaccaataat gtgctgttat gtatacatgc aaaaacaagg agagaatgct
     8821 tatgtacaaa acaatcatca tgccagtgga tgttttcgaa atggaattga gcgacaaagc
     8881 gattcgccac gctgaattcc tcgcccagga tgacggagtt attcatctac ttcacgtact
     8941 accggggtca gccagcctga gcctgcaccg ttttgccgct gatgtacgtc gttttgaaga
     9001 gcatctgcaa catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga
     9061 tccttcccgc attaagcaac atgtccgttt tggtagtgtg cgggatgaag ttaacgagtt
     9121 agcgaaagaa cttgatgcag atgtagtagt gattggttcg cgtaacccat caatttcaac
     9181 tcatctgttg ggttctaacg cctcgagcgt aatccgccac gccaatttgc cggtgctggt
     9241 tgtgcgttaa ttgccagaat aagtctcccg ccctgcccgg agccatccct gcagggcttt
     9301 ttaatgcaat aaaaaagccg ccaggtttga ccccggcggc taagcaatta caggtgaatc
     9361 ttacttcttc ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt
     9421 tggcaccttc gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg
     9481 ctgcgaacac gcctttgacg ttggtttcgc atttcgcatc aatgataatc tcgcccatgc
     9541 gattacgttc gactgcgcct tccagccagt tggtgttcgg cagcagacca atctgcacga
     9601 aaataccggc cagttcgatg ttgtgaatat cgccgctgac acgatctcga tattccagac
     9661 ctacgacttt gctaccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga
     9721 cgtttttcag gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa
     9781 attccagcag cgttacgtgc tcaacgatac ccgccaggtc aattgccgct tccacgccgg
     9841 agttaccgcc gccgataacc gctacgcgtt tacctttaaa cagcgggccg tcgcagtgcg
     9901 ggcagtaggt cacgcctttg gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc
     9961 attttgcacc ggtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt
    10021 caatctgatg caggccacct tcaaccgctg ccgggatcag tttgctggcg ctctggctgt
    10081 cgatcacatc aacgtcgtat tcatcaacgt gaactttcag cgctcctgcc agtttctggc
    10141 cttcggtctt cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac
    10201 caaaacgttc gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg
    10261 ctgcacccgc cgggccggaa ccgacgatta atacgtcata agcatcacgc ttgttcagct
    10321 cttctgccgc acgtttttcc gcaccagtat caattttggc aacgatttca gtcaacgtca
    10381 tgcggccctg accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc
    10441 gatcggtgat ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt
    10501 tcagtacgct catcaggttc agcgcctgca ccacgtccgg gcagttgtgg caagagagcg
    10561 agtaataggt ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact
    10621 gcgcttcttt cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact
    10681 cgtggcccag cggggagcct gcaaaacgtg gcccctggtt agaacctggg ttggtgatca
    10741 ggaaagacgg cttacgcacc ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca
    10801 gttctgcgat ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg
    10861 tggcaattaa ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca
    10921 tatttgtgtc gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctctg
    10981 cggctacctg gatgcagctt gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc
    11041 cgaagaatta ggtgaatttc cgaagtattt cgcgccggag ttagtagcgc gaaagacaaa
    11101 ggaaatttag attttaccaa ccaggtccag agacggagcc agagttgctt caccttcttt
    11161 ccatttagcc gggcaaactt cacctgggtg agaagctacg tactgtgctg ctttgatttt
    11221 acgcagcagg tcagacgcgt cacggccaat gccttcagcg gtaacttcga ttgcctggat
    11281 gataccctgc gggtcaacaa cgaaggtcgc acggtcagcc agaccttcat cttcacgcat
    11341 gttgtcgaag ttacgggtca gggcgccagt cgggtcgccg atcatcgcat atttgatttt
    11401 agcgatggtt tcagagctgc tgtgccatgc tttgtgggtg aagtgagtat cggtagatac
    11461 tgcgtatacg tctacgccca gtttctgcaa ttcttcgtaa tggtcagcaa cgtcacccag
    11521 ttcggtcggg catacgaaag taaagtcagc cgggtagaag aagaagacgc tccagcggcc
    11581 ttcggtatct ttttcggtga tttcgatgaa ttcgccgttt ttgaatgcct ggtttttaaa
    11641 aggtttaatt ttggtgttaa tcaaggacat ctatacttcc tccgtgtttt cgatgagatg
    11701 taaggtaacc aatttttgcc gattcggcta atgcgtttcc attatcaaat cgataagttt
    11761 taccttacaa ccttcgtaag acaactttgt gaactttgca ccgccagcgg cgataaaaag
    11821 taaaatggcc ccctttccag gtgaccgtgt aatctgaact accctttggt atcttcagcg
    11881 cctgcctgca ggctgaggtg ttgcccttac aaatgcaaca acgacatgga ttacaacacc
    11941 ctcataaaca aagggcaatc acctgatcta agctcttacc tatgacagtg ataggttatg
    12001 ccttttactc gacttttgca ctgactgaaa aggacaaatt aatgttaaaa aagatacttt
    12061 tactggctct gcttcctgca atcgccttcg cagaggaact tcctgcccca gtaaaagcga
    12121 ttgaaaaaca gggtattaca atcatcaaaa cgttcgatgc ccctggagga atgaaaggtt
    12181 atctcggtaa gtatcaggat atgggcgtca ccatctacct gactccagat ggtaagcacg
    12241 ctatctctgg ttacatgtac aacgaaaaag gtgaaaacct gagtaacacg cttatcgaaa
    12301 aagaaattta cgcacccgcc ggacgtgaaa tgtggcaacg gatggaacaa tcccactggc
    12361 tcctcgacgg taaaaaagat gcgccggtca ttgtctacgt cttcgccgat ccgttctgcc
    12421 catattgtaa acaattctgg cagcaggctc gtccgtgggt agattctggc aaagtgcaat
    12481 taagaacatt gttggttggg gttatcaagc cagaaagccc ggcgacagca gcggcaattc
    12541 ttgcctccaa agatcccgca aaaacctggc aacaatatga agcctctggt ggcaagctta
    12601 agctaagcgt gcctgcaaac gtaagcacag agcaaatgaa agtgttaagc gacaacgaga
    12661 aactcatgga cgatctgggg gcaaatgtca caccagctat ctattacatg agtaaggaaa
    12721 ataccctgca acaagccgtc ggattacccg atcagaaaat gcttaatatc attatgggga
    12781 ataaataaaa gataattcat ggatggttga aatatatcct tgcttttcaa ccatcctaaa
    12841 aacgcatgaa ttgctcgtaa cccacctgac agctatgcta ttttagaaaa tcttaatcca
    12901 aagttaagca gaaaaacata atacatataa atacaaaaac attattgatc aagtgaatga
    12961 tttatggaag agacaataca acggagtatg ttatggctaa tctttatgat ctaaaaaaat
    13021 ttgacctcaa tttactggtc atatttgaat gtatttatca acatctcagc attagtaaag
    13081 cggcagaatc attatatata acgccctctg ctgttagtca gtcgttacaa cgtttacgtg
    13141 cgcaatttaa tgacccgtta ttcattcgct cagggaaagg tatcgcgccg actacaacag
    13201 ggttaaacct gcatcaccat cttgagaaaa atctcagagg ccttgaacaa acgatcaata
    13261 tcgttaataa gtctgaacta aagaaaaatt ttattatcta cggtcctcag cttatttctt
    13321 gtactaataa caacatgcta atccgctgtc ttagacaaga tacttcaatc gatatagaat
    13381 gccatgacat cctgctttca gctgaaaatg ccgaagaact gttggttcaa cgcaaaactg
    13441 acctggcaat tacactgcaa ccagtaatta gtcgctctgt catctgcatg ccattacaca
    13501 ctattcgcaa ttcccttata tgtagcaaca aacatcctcg tattactgat accagtaact
    13561 atgaacaaat tatcgccgaa gaattcaccc tgttgatatc taaatcagca gggattgacg
    13621 aaattcaaat ggacatagat gaacgtttta taaaccgtaa agtttcattt cgcagttctt
    13681 ctttgttgac tataattaat agcatttctg ttactgactt attaggcatt gtgcccaccg
    13741 aactgtacga tt
//
LOCUS       DAZGHF010000124        13510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGHF010000124 DAZGHF010000000
VERSION     DAZGHF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.125"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(134..210)
                     /locus_tag="ACHK3A_003865"
     tRNA            complement(134..210)
                     /locus_tag="ACHK3A_003865"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(174..176),aa:Met,seq:cat)
     gene            419..1516
                     /gene="mltA"
                     /locus_tag="ACHK3A_003866"
     CDS             419..1516
                     /gene="mltA"
                     /locus_tag="ACHK3A_003866"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO8741891.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1593..2399
                     /gene="tcdA"
                     /locus_tag="ACHK3A_003867"
     CDS             1593..2399
                     /gene="tcdA"
                     /locus_tag="ACHK3A_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO8741892.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2450..2893)
                     /gene="csdE"
                     /locus_tag="ACHK3A_003868"
     CDS             complement(2450..2893)
                     /gene="csdE"
                     /locus_tag="ACHK3A_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO8741893.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2893..4098)
                     /gene="csdA"
                     /locus_tag="ACHK3A_003869"
     CDS             complement(2893..4098)
                     /gene="csdA"
                     /locus_tag="ACHK3A_003869"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO8741894.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATTLKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPHLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTPQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4290..4517
                     /gene="ygdI"
                     /locus_tag="ACHK3A_003870"
     CDS             4290..4517
                     /gene="ygdI"
                     /locus_tag="ACHK3A_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO8741895.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4868..5785
                     /gene="gcvA"
                     /locus_tag="ACHK3A_003871"
     CDS             4868..5785
                     /gene="gcvA"
                     /locus_tag="ACHK3A_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO8741896.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5804..6199
                     /gene="ygdD"
                     /locus_tag="ACHK3A_003872"
     CDS             5804..6199
                     /gene="ygdD"
                     /locus_tag="ACHK3A_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO8741897.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6192..7292
                     /gene="rlmM"
                     /locus_tag="ACHK3A_003873"
     CDS             6192..7292
                     /gene="rlmM"
                     /locus_tag="ACHK3A_003873"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO8741898.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7336..8067)
                     /gene="fucR"
                     /locus_tag="ACHK3A_003874"
     CDS             complement(7336..8067)
                     /gene="fucR"
                     /locus_tag="ACHK3A_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO8741899.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVAASLVTA"
     gene            complement(8125..8547)
                     /gene="fucU"
                     /locus_tag="ACHK3A_003875"
     CDS             complement(8125..8547)
                     /gene="fucU"
                     /locus_tag="ACHK3A_003875"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO8741900.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8549..9967)
                     /gene="fucK"
                     /locus_tag="ACHK3A_003876"
     CDS             complement(8549..9967)
                     /gene="fucK"
                     /locus_tag="ACHK3A_003876"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO8741901.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10076..11851)
                     /gene="fucI"
                     /locus_tag="ACHK3A_003877"
     CDS             complement(10076..11851)
                     /gene="fucI"
                     /locus_tag="ACHK3A_003877"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO8741902.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQIFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11884..13200)
                     /gene="fucP"
                     /locus_tag="ACHK3A_003878"
     CDS             complement(11884..13200)
                     /gene="fucP"
                     /locus_tag="ACHK3A_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO8741903.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3275 a         3337 c         3554 g         3344 t
ORIGIN      
        1 tggatggctt tgatggaggg atattaacgg tttactcatt gtctcaggct tcactgaaac
       61 gggtaaggaa gcgtaaaaac gaaaaaaccg caagccaata gtagctttgc ggtttgtcag
      121 cattctaata aattggttgc gggggccgga tttgaaccga cgaccttcgg gttatgagcc
      181 cgacgagcta ccaggctgct ccaccccgcg tccgtggatg cgcactatac tctgctcgcg
      241 ttttcatgca accttttttt taccccgatc acggattcgg catgattttg aacaaaaaca
      301 aatcactaaa gcctattttt tgtgcaaaat ttgcgtcagg gagaggctgc tcatttcatt
      361 aaacgcggtg gtttgttatc ttcgttgcgc cttatttttt aacctgaaga agagaacaat
      421 gaaaggacgt tgggtaaagt accttcttat gggcacggtt gtggcaatgc ttgccgcctg
      481 ctcttccaaa ccaaccgatc gcggacagca atataaagac gggaaattta cccagccttt
      541 ctctctggtg aaccagccag atgccgttgg cgcgccgatt aatgccggtg attttgccga
      601 gcaaattaac catatccgta attcgtcacc gcgtctgtat ggcaaccaga gtaatgttta
      661 taacgcggtg caagagtggc tgcgcgcagg cggtgatacc cgcaatatgc gccagttcgg
      721 cattgatgcc tggcagatgg aaggtgtcga caactatggt aacgtgcagt ttacaggcta
      781 ttacacgccg gtaattcagg cgcgccatac ccgtcagggc gagttccagt atccgattta
      841 tcgtatgccg ccaaaacgtg gtcgtctgcc gtctcgtgcg gagatctacg caggagcgct
      901 gagtgataaa tatattctcg cttacagtaa ctccctgatg gataacttca ttatggatgt
      961 gcagggtagc gggtatatcg actttggtga tggcagtccg cttaactttt tcagctatgc
     1021 agggaaaaac ggtcatgcct atcgcagcat tggtaaggtg ttgatcgacc gtggcgaagt
     1081 gaaaaaagaa gatatgtcga tgcaggcgat tcgtcactgg ggcgaaacac acagtgaagc
     1141 ggaggttcgc gagctgctgg aacagaaccc gtctttcgtc ttctttaaac cgcaatcttt
     1201 tgcaccggtg aaaggggcaa gtgcggtgcc gctggttggc cgtgcttctg ttgcctccga
     1261 tcgttcaatt attccgccag gtactacctt gctggcagaa gtgccgctgc tggataataa
     1321 cggcaaattt aacggtcagt acgaattgcg tctgatggtg gcgctggatg tcggtggcgc
     1381 aatcaaaggc caacacttcg atatctatca agggatcggg ccggaagccg gacaccgcgc
     1441 aggttggtac aaccactatg gacgtgtctg ggtgctgaaa accgccccgg gcgcaggtaa
     1501 cgtctttagc ggctgatgtg atattctgta tgccgttttt acggatatga cagggtgagg
     1561 ggtaacctca ccctttttaa ttctgaggtg ctatgtctgt ggtaattagt gatgcatggc
     1621 gtcagcgttt tggtggcaca gcgcgtctgt atggtgaaaa agcattgcaa ctgtttgctg
     1681 acgcgcatat ttgtgtggtt ggtatcggtg gtgtcggttc ctgggcggcg gaagcgctag
     1741 cgcgcacggg gattggcgca atcacgctta tcgatatgga tgatgtgtgc gtcaccaata
     1801 ccaatcggca aattcacgct ctgcgcgata acgtcgggct ggcaaaagcg gaagtaatgg
     1861 cggagcgtat tcgccagatt aacccggagt gccgtgtaac agtagtggat gacttcgtga
     1921 cgccggataa cgtagcgcaa tatatgaatg ccggttattc gtacgtgatt gatgctattg
     1981 atagtgtacg gcccaaagcg gcgctgattg cttattgtcg gcgcaataaa atcccgctgg
     2041 tcacgaccgg tggcgctggt gggcagattg acccgacgca gattcaggtt accgatctgg
     2101 cgaaaacgat tcaggacccg ttggcggcga agttgcgcga gcgcctgaaa agcgattttg
     2161 gcgtagtgaa aaacagtaaa ggtaagctcg gcgtggattg cgtgttctct accgaagcgc
     2221 tggtgtaccc gcagtcagac ggcaccgtct gtgcgatgaa agccacggcg gaagggccaa
     2281 agcggatgga ttgtgcctca ggatttggcg cggcgacgat ggtgaccgcc acttttggct
     2341 ttgttgcggt ttctcatgcg ctgaagaaga tgatggcgaa agcggcgcgt caggggtaaa
     2401 caatcaggtc ggatgcgacg cgaacgtctt atccgacctg gttctgtgct taaacctgct
     2461 tcgccgcagc gataatcgcc tcgcttaacg cattcaaccc ctggctgcgt gaggcgctaa
     2521 gttgcgcacg taatcccagc tcatcaaaca atgccagcgg cgactgtgcc tgcaattcgg
     2581 cggcggtttt cccctcaacg gcagtcaaca acaccgccaa caggccgcgc acaatgcgcc
     2641 cttcgctgtc gccaaagaaa tgcatcttgc cgttttcagc cactgtatat cccagccaga
     2701 cgcggttttc gcatccggca atctctttag cctgcgcttt taactcctct ggcaaagccg
     2761 gaagctgttt ccccagcatg atcaactggc gatatttatc ttcccattgc gtcaacggtg
     2821 cgaaggtatt gcgtaacgtt tctgcggtta cggttgtgcc gaacggatgt ccggcgaatt
     2881 gcgggtttgt cattaatcca ccaataattc cagcgcgcgg tcaacggcat tcaccagcgc
     2941 atccacatca ctctttgtat tatatggcgc aaatgaggcg cgcagtgtgc cggttacgcc
     3001 taattctgcc agtagcggct gagcgcaatg ctgcccggcc cgcagggcaa taccgtactc
     3061 cgccagcagc gtcaccatat cgctgtgatg aacgccagca aaatcaaagg ccagcaggct
     3121 ggaatcctgg cagcgaaatg agcggaagcc aggacgtttt gccagcgcat cttccgccag
     3181 cgttgctaag ctacggctcc agctttcggc ctggttgata tcgtaatctg ccagccattc
     3241 cagcgccgcg cttaatccta tgacaccagc gacatttggc gttccagctt ccagtttcca
     3301 cggcgcagat tgaggcgtga agccgtcaaa actcacttcg tgaaccattt tgccgccgcc
     3361 cagccagggc gacatcgctt ccagcagttc tgatttgcca tacagcacgc cgatacctgt
     3421 cgggccatac agtttgtgac ctgaaaaagc atagaaatca atatccagtt gctgaacatc
     3481 cgcggggaaa tgcaccgccc cctgagcacc atcaaccatc accaccatcc cggctgaatg
     3541 agcaaaggta atcgctcgcg ccagatccgg gcaaccgcca gtgacgttcg acatttgtcc
     3601 cagagcaaga attcgactac gaggtgtgat cagttctggc aacaaatcga catccggcag
     3661 tcgctgcgca ttaagcggca atttcaccac tttggctcca gtttgttggg cgaccatcag
     3721 ccaggggacg aggttggcgt ggtgttctgc cacgctgaca ataatctcat cgcccggttg
     3781 cagatgcgga cgcgcatagc attgcgccac catgttgatg gattcagtgg tgccgcgcgt
     3841 ccagacgata gttttgtcat ctggtgcatt cagtaattgc gccactttct cccgtgcagc
     3901 ttcataacgc gcggtcaggc gttgggcttc ggcaaactgg ctgcgatgga cgtttccggc
     3961 gctcaggctg taaaactgtc gggtggcttc aaccacggct tcaggtttaa gcgtggtcgc
     4021 ggcgctgtcg agatagacgc ccgcatcctg tagcgcggga aactgggcgc gaaactgcgc
     4081 gggattaaaa acgttcatgg tactcctcgg cttgatgcac cgatcgtgac gcaaaattca
     4141 ctttgataca aggagagtgc taaccatcag aattatctgg atatcctggc gaagccgcgt
     4201 cagtaacata tgctgaatag tgttaggcgt taattttagc ctgttaatca atactattaa
     4261 gatttattaa tcgctttaag gagaagatga tgaaaaagac tgccgcaatt atttctgcct
     4321 gtatgctgac ttttgccctg agcgcctgtt ccggttcgaa ctatgtgatg cacaccaatg
     4381 acggacgtac catcgtctct gacggcaaac cacagactga taacgatacc ggtatgattt
     4441 cgtataaaga cgctaatggc aacaaacagc agatcaaccg taccgacgtg aaagagatgg
     4501 tcgaactgga tcagtaattc gcgatcgcaa ggtaaaaaaa agcaccgcaa ttaggcggtg
     4561 ctacattaat cactatggac agacagggta aatgtacagg aagtgaaaaa aggtagcttt
     4621 gctaccatgg tctgaatcgc agaccaattg caaacacaac aacacaacat cacaaccgta
     4681 agccaaaagt tcaccagaac acgcattccg ataaaacttt tcgttccggc tcaggaagtg
     4741 ccgccactat aggtatttgc tggtagaagc tcaacggaca atttataatg gctcagatta
     4801 aaaaaactaa taggttacat agtgtaatct aattgttaaa ttcatttaac atcaaagttt
     4861 aaaagccatg tctaaacgat taccaccgct aaatgcctta cgagtttttg atgccgcagc
     4921 acgccattta agtttcactc gcgcagcaga agagcttttt gtgacccagg ccgcagtaag
     4981 tcatcaaatc aagtctcttg aggattttct ggggctaaaa ctgttccgcc gccgtaatcg
     5041 ttcactcctg ctgaccgagg aagggcaaag ctatttcctc gatatcaaag agatattttc
     5101 gcaattaacc gaagcgacgc gtaaactcca ggcccgtagc gctaaggggg cgttgacggt
     5161 cagtttactc cccagtttcg ccattcattg gttggttccg cgactttcca gctttaattc
     5221 agcttatccg ggaattgacg ttcgaatcca ggcggttgat cgtcaggaag ataagctggc
     5281 ggatgatgtt gatgtggcga tattttatgg tcggggcaac tggccggggc tacgggtgga
     5341 aaaactttac gccgaatatt tattgccggt gtgttcgccg ctactgctga ctggcgaaaa
     5401 acccttgaag accccggaag atctggctaa acatacgtta ttacatgatg cgtcgcgtcg
     5461 tgactggcag acatataccc gacagttggg gttaaatcat atcaacgttc agcaagggcc
     5521 aatttttagc catagcgcca tggtgctgca agcggctatc cacgggcagg gagtggcgct
     5581 ggcaaataac gtgatggcgc aatctgaaat tgaggccgga cgtcttgttt gcccgtttaa
     5641 tgatgttctg gtcagtaaaa acgcttttta tctggtttgt catgacagcc aggcagaact
     5701 gggtaaaata gccgcctttc gccaatggat cctggcgaaa gccgctgctg aacaagaaaa
     5761 attccgcttt cgttatgaac aataatttac gtagggtacg accatgacca gccgttttat
     5821 gctgattttc gccgccatta gcggcttcat ttttgtagct ctgggcgctt ttggcgcgca
     5881 tgtgttaagt aaaaccatgg gggccgttga gatgggctgg atccagaccg gcctcgaata
     5941 ccaggcgttt catacgctgg cgatcttagg tctggcggtg gcaatgcagc gtcgcatcag
     6001 tatctggttt tactggagta gcgttttcct cgcgttaggc acggtgctgt tcagcggcag
     6061 cctttattgc ctggcgctgt cccatctgcg tttgtgggcg tttgtcacac cggttggcgg
     6121 cgtgagcttc cttgcgggct gggcgttaat gttagttggt gctatccgtt taaagcgcaa
     6181 gggcgtaagt catgaataag gttgtattgc tgtgccgtcc gggctttgaa aaagagtgcg
     6241 ccgcagaaat taccgataaa gccggccagc gggaaatttt cggttttgcc cgcgtgaaag
     6301 agaatgcggg ttatgtcatt tatgaatgtt atcaacctga tgatggcgat aagttaatcc
     6361 gtgagctgcc gttcagttca ttaatttttg cccgccagtg gtttgtggtg ggggaactcc
     6421 tgcagcattt gccgccagaa gatcgtatta cccccattgt cggcatgtta cagggtgtag
     6481 tagagaaggg cggtgaactg cgtgttgaag ttgccgatac caacgaaagc aaagagttgc
     6541 tgaaattctg ccgtaaattc accgtgccgc tacgcgctgc cttgcgcgat gcgggtgtgc
     6601 tggcgaacta tgaaacgccg aagcgtccgg ttgtgcatgt attctttatt gcaccaggct
     6661 gctgctatac cggttactca tacagcaaca ataattcgcc gttctatatg ggcattccgc
     6721 gcctgaaatt tccggcagat gcgccgagtc gttccacgct caaactggaa gaggcatttc
     6781 atgtgtttat tcctgcggat gagtgggatg aacgcctggc gaacgggatg tgggcggtgg
     6841 atttaggcgc ttgcccaggc ggctggacct accaactggt gaagcgcaac atgtgggttt
     6901 attccgtcga caacggcccg atggcgcaaa gtctgatgga taccggacag gtgacgtggc
     6961 tgcgggaaga cggtttcaaa ttccgtccga cacgcagcaa tatctcctgg atggtatgcg
     7021 atatggttga aaaaccggcg aaagttgcgg cattgatggc gcagtggctg gttaatggct
     7081 ggtgccgtga aaccattttc aacctcaaac tgccgatgaa aaaacgctac gaagaagtgt
     7141 cacacaatct ggcgtatatt caggcacagc ttgatgaaca tggcataaat gctcagattc
     7201 aggcgcggca gttgtatcac gatcgcgaag aagtgacggt gcacgtccgc cgtatctggg
     7261 ctgcggtggg tggtcgtcgc gacgagcgat aacaggcaag gccggggcgt cgtttccggc
     7321 ctgctatcct ttccgtcagg ctgttaccaa agaagctgca acctggcgct catcagaaat
     7381 aatgtgcgtg acctcatcca gatgcccgat gcgggcttcc cctgaacgat taaatttact
     7441 tttatcaatc aataacaacg attgcgcggc acgttttaat agcatcgatt tgtaatcagc
     7501 gttgatggca ttggagtccc acagtgcgcc gctgctatcg atcccttcac aagaaaaaat
     7561 aaacagatcg atttccagcg atttaagctg ggaaatcagc gagggattga cgtaacaacc
     7621 atatttgcgc tcaagtgtgc cgccggaact gatcagttga atgcgttcgc gtttaccgag
     7681 ttcatggcaa atcggatggc tattggtgaa gacctggatg ttgatgtcag gcaactggcg
     7741 tgccagatac cagcaggttg aacttgcgtc taaggctatt accatccctt cttcaatcca
     7801 cgcgagcgcc tcgcgcgcga tatctgcttt atgcgcataa tggcttttca gccggatgtg
     7861 aaagggatcg ccactgtctt gattttgacg gtggatatat ttagcgcgcc catgattgcg
     7921 cagaatttta ccctgcgtct gtaattcatt gagatcgcga cgaatggttt ctttactgac
     7981 ctttagctgt tcagagagag cttccgtggt caggctggta tggttcagca gcaggtcgac
     8041 tatcgcttgc tggcgtgccg ctttcatatt tttcacccca tcattcgata accgcccgcg
     8101 tcatgcgggc gtaccggtat gagattacgg tgttacccct ttttttaaaa gaatattccc
     8161 gtacttcgct cgttcgcctg tgataacgat cgcaaaggct ttttgtgccc gttcataaaa
     8221 cgcaaaacga ttgatgcgga tgatgtcagg acagggggct tgtagtgaaa gcgcattacg
     8281 ataacgtcgt tctacttcag gatctagagt gtcaccttct accgccgcca tcatcaccag
     8341 cggtggtgca taactgtcca gctcaaataa cgggataatc gcctggagca agtcgctcac
     8401 caacaggcca tcagcgcgga tcacctgcgg tcccatcgaa tgggcgggaa agtgagcatc
     8461 ggaaaaaata atttcatctc catgccccat ctctgccagc actttcaata gttcgggaga
     8521 aattaacggc gaaattgttt tcagcatttc acacctcctc tataaattca ggttcagttt
     8581 gcgggtagaa ataacggtac tgataatgaa tctgcgcgcg ggcttcttcc ggactgttaa
     8641 attcccctac gccataccaa ccaaacagcg cagctcctgc gacggtcgtt tcggcatcgt
     8701 cgagaacttt taccggaata tcaagcatat tggctttaat ctgattccac aatgtgttgc
     8761 gacttcctcc gccgactaac aataattcag aggctttaaa gtgcccgatt ttttccagca
     8821 tctgtagatt gcgctgtaat tgcgtggtta acccttccag cgccgcgcga tagaaatgcc
     8881 cccgcgtggt attaagcgtt acgccttgcc agccagcgtt ctggcacgac aataaatcac
     8941 actgcatttt cactccatct gcgccaggcg cgatcaggcg agcttcttca atcaatattt
     9001 gccagggagt ttcagctgtc cagaacagtt ttctcaccca ttccagcacg ccggatgcca
     9061 gccattgcat acctgggtta tacaaccctg cctggctatc cagttcgcaa gtagaaccgg
     9121 cgtactgact taacagcgaa gtatcaacct gggcgctgcg gaccattaaa atttcccatg
     9181 taccggaaga gagcacgggt tcattttgtt cggcaccagc gccaaaaagg gcgaactggg
     9241 tatcgtgacc tgcggaaatc accggtatgc caacgggtaa gccgagcatt gctgcggcgc
     9301 tgttctgtag cgtaccaatc tgttcacccg cttccaccag acgagggaag agtcggcgtg
     9361 gaataccggt ggcttgtaaa atttgcggac tgaaatcgcg ttgctggata tccagcatct
     9421 ggctggttcc ggccatcgta atatcagtag tgaattcgcc ggttaaacgg tggttaatca
     9481 gtgacgaaat aaagagccag gcgtgcgcgc gttccagcag ttgtggatga ttttctttca
     9541 accacaccaa cttatataac gtattgaaac taaaggctcc gactccagaa atagcctgca
     9601 actgctgtgc ggagatcaac cgttcaatat tgtccattac cgcggctgtt cgcggacatt
     9661 tccagctaat gatcggatag agcagattgc cttgcttatc taccagagcg ccatccacac
     9721 caaaggtggt gacggcgata ccgcggatat ggcaatcagt cagttcacta ttgatttgcc
     9781 gacagcaatc agcaaagcgt tgcaaaatgg cgtctaaaga ccactggtgc caggtgttgt
     9841 tttccatcgc gatatcgctg gcattaggcg ttgaggcgcg ggcaacaatt tttccctgcc
     9901 gattaaccgc gatggccctg acattggtcg cgccacagtc gagtaccagg ataacttctt
     9961 gtttcataat cgctcctgca atatagccgg ataacattgc ttatccggct aaccactctt
    10021 gcttgttcag gcccggtagc cggagcgacc gggcatcaca tcagggagtc atgtattaac
    10081 gcttgtacaa cgggccgtag ttctggcaag cgcggtaatc ctggccttca atatccatgc
    10141 cgtgcgcggc ccaggcagaa ggacgataca ctttggtctc ttcaacgttg tgcatacata
    10201 ccgggatacg cagcatggag gcaagagtga taaagtctgc gccaacgtgg ccgatggtca
    10261 gaactccgtg gttagcgccc cagttcgcca ttaccgagta cacatccgta aacggccctt
    10321 taccggtgag gcgcggtgca aaccaggtgg ttggccaggt tgagttggtg cgtttgttga
    10381 ggatgtcatg cacatccttc ggcaattcca cgctccagcc ttccgcgatt tgcagtaccg
    10441 gtcccaggcc tttgatgatg ttgacacgag tcatggtgaa cgggacgccg ccttcggtaa
    10501 ggaagcggga agagtaaccg ccgccacgga agtattcgtg gatcgccggg caccattcgg
    10561 tagcggcgag gcaagcgtca gcctcttgct gagagatttc ccagtgcggc ttcatcgtcg
    10621 gtttaccttc gctgtcgcgt tgtttacagg agccgtccag cgcagcagaa ccggagttga
    10681 tcaaatggat gatgccgtgt tctgccagcc cgtccagttt atgccccgtt acacgctcaa
    10741 ttgcttctgg tgaccagtag gtacgcacat cggcaaatat ctgagcggtg ccggtgagct
    10801 ggtggcccat taacattgcc acgccgttaa gactgtcgtt ttcggtcgcc acgacaaacg
    10861 gtttgcgcac gccattccag tcaaatgaac tgttgaggat cgcttcagcg gtatcgccat
    10921 tgggatattg atcggtccag tgacgttgcc cctggaagcc tgcagcgatg gcgttgtagc
    10981 caagtgattc ttccacgcgg ccaatatcgg ccagtttgct gttgccttgc atcatgtcgc
    11041 ggatacacat cgccatcagt aaactttcgc gcagaactgc gcggctttgc tcggcattgc
    11101 gttgatactg tttgttattt tcatcttcgc catagcggaa gtttttatca gcccaggcca
    11161 gcgccatttc cagttcggct tcgtcgtaaa tcttctgatc gatacggcga cgcagttcgg
    11221 tcatatccac cgcctggact ttcattccca gccaggattc aaagaagttg tgatcaacga
    11281 tggaaccggc gatacccatc gaaacgccgc ccagcgacag atagctttta cctttcatgc
    11341 tggcgactgc taaaccggcg cgggcaaagc gcagcagttt ttcttcaaca tcggcaggaa
    11401 tcgatgtgtc atcggcatcc tgaacgtcat gaccgtaaat ggagaatgcc gggatgcctt
    11461 tctggctgtg agctgccaga gccgctgcca ggtaaacagc gccgggacgt tcagtgccgt
    11521 taaagcccca aatggctttc gggcgggttg gatccatatc gatggtttca ctgccatagc
    11581 accagcaagg ggttaccgta atggtgaggc ctacattctg actgctgaat ttttcttcgc
    11641 aagcagcggc ttcagccata cccgcgatac aggtatcgga aatgacacac tcgacggcag
    11701 ctccacaggc atggcgcagt ttctcggtca gcagtgcagc cgtggctttc gccatattca
    11761 ttgtttgttc ttcaagcgac tcacgaacac ccatgcgacg accgtcaata accgggcgga
    11821 taccaatttt cggtaagctg atttttttca ttacagattc ctcactttat tcggaaaata
    11881 tgttcagtta gttgctgttt gagaacggaa acgggcaaag ataaagatga ccgcgaagca
    11941 gagtgcgggg atcagttcag cagtggggat gttgcccgcc gcgtcactga caaaacccat
    12001 gaccggagtg acaataccgc cgccaatgat ggtcataacg atgaaggacg aaccgtattt
    12061 ggtgtcctgg ccgagattct taatgcccag cgagaagatt gttgggtact gaatcgacat
    12121 aaaggcgctg cataaagtca gggctattaa gcccacatga ccgccagcga aggctgagat
    12181 caggcacagt gccatagcga ttaatgcgta ggctgccagg actttgtgtg gtgcgaagcg
    12241 actgatgagc caggtaccgg tgaaacgacc aataaagaag cacaccatgg ttccggttaa
    12301 atagttagcg gcaaagcctg cggtcatacc tggaatttct tctacagcgt agcgaatcaa
    12361 atagctccag caggccgttt gcgcgccgac atagcagaat tgcgctaata ccgcccagcg
    12421 ccagtgacga atacgcgcca gacgagaaag cgatgcggag aacgatcctt gtttggcgtc
    12481 actgtgatta tcactctgca atgccgggaa tttcgtcagc atgatcagca gggcgaccag
    12541 taacacgata gccacgatga tcatataagg tgtctgtacc gataatacca ggctgtgttt
    12601 atacgcactc aattgctctg gagacatttt atcgagaacg tcttgcgatt gatgtggcac
    12661 gttagacaaa ataagacttt gtccaaagac aaccgcgata attgcgccaa acgagttaaa
    12721 tgtttgcgca agatttaagc ggaagtgacc actgctttcc ggccctaata ccgtaacaaa
    12781 agggtttgcg gcagtttcca gacaacctaa tccagctgca ataataaata ggccaactaa
    12841 aaataaggtg tagttcatta tttctgcagc gggccagaat aatgcagcgc ccaaggcata
    12901 taaaaataat ccggtaataa tccctgcttt ataactgagt tttttcatca atatcccagc
    12961 agggattggg ataatgaaat aaccaaagta aaaggccgat tggatcaggc cagcctggaa
    13021 atttgtcagc gtaaaagcct gctggaattg aggtaataaa atgtcgttaa ggttattggc
    13081 taccgcccaa agaaaaaaca gtgagcacag cagcgcgaat ggaataatgt aacttctgct
    13141 ttgccctgca tctttatcta ccgcacggta actctgcgtt tgtattgatg tgtttcccat
    13201 agcatcctct taggttcaga agcttaagca cgtaatcggt tatgttttca aaagtgcaaa
    13261 accagcgagt tcgggcttca tatcagtgaa tatttgcctg tcagaaatga actcatggat
    13321 tcattttcaa ataaaaaacg ctctgtaata tgacggcggt cacacttatt agttgggagg
    13381 tgactttatg tgactaccat cactttaata atcgaaattt catgcccgtt tcagaaaata
    13441 aatcataaaa aacacaaacc cgatgaccgt attaattgtt tgtgccgaaa acgggcattt
    13501 ctggcgaggg
//
LOCUS       DAZGHF010000125        13311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGHF010000125 DAZGHF010000000
VERSION     DAZGHF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.126"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(149..451)
                     /locus_tag="ACHK3A_003879"
     CDS             complement(149..451)
                     /locus_tag="ACHK3A_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741904.1"
                     /translation="MSHCTSDGRFVCNDGSLSQSKRFCSGYGVAPAKRQVKSPVTTKL
                     VQKKKSAAVRKVSQPSVAGKNEVVPVTEPRKPTCAPLYMASKPGFTHLPICSGNQY"
     gene            complement(798..1802)
                     /locus_tag="ACHK3A_003880"
     CDS             complement(798..1802)
                     /locus_tag="ACHK3A_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO8741905.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKFGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARRISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEPLQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQPGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFMMRLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWVITTQQKEYQA
                     "
     gene            complement(2060..2491)
                     /gene="tssE"
                     /locus_tag="ACHK3A_003881"
     CDS             complement(2060..2491)
                     /gene="tssE"
                     /locus_tag="ACHK3A_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO8741906.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFIPEGRVLLRHLKQQRYVQT"
     gene            complement(2494..3030)
                     /gene="tssJ"
                     /locus_tag="ACHK3A_003882"
     CDS             complement(2494..3030)
                     /gene="tssJ"
                     /locus_tag="ACHK3A_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO8741907.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWMQPGGSVTVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            complement(3011..4111)
                     /gene="tssG"
                     /locus_tag="ACHK3A_003883"
     CDS             complement(3011..4111)
                     /gene="tssG"
                     /locus_tag="ACHK3A_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO8741908.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYTGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYLGSRLDVRLQLCVERSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            complement(4066..5829)
                     /gene="tssF"
                     /locus_tag="ACHK3A_003884"
     CDS             complement(4066..5829)
                     /gene="tssF"
                     /locus_tag="ACHK3A_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO8741909.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVEKVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENISLPAGISGFDIE
                     VVLSQSWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVAGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEADRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPISVCNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            complement(5963..7561)
                     /gene="tssA"
                     /locus_tag="ACHK3A_003885"
     CDS             complement(5963..7561)
                     /gene="tssA"
                     /locus_tag="ACHK3A_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO8741910.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKNIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADDVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLVELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNSGSS"
     gene            complement(7561..10950)
                     /locus_tag="ACHK3A_003886"
     CDS             complement(7561..10950)
                     /locus_tag="ACHK3A_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO8741911.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTSPRYVTVQDI
                     RHTLRRQHGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHM
                     EPARFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLHSEGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDSVEETD"
     gene            complement(10943..12091)
                     /locus_tag="ACHK3A_003887"
     CDS             complement(10943..12091)
                     /locus_tag="ACHK3A_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO8741912.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFFKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREIMDK
                     FCQLPAVLPVNVTLITDQPDSEIRSAFVSAWEVLFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCFGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(12095..12361)
                     /locus_tag="ACHK3A_003888"
     CDS             complement(12095..12361)
                     /locus_tag="ACHK3A_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO8741913.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(12392..13069)
                     /gene="tli1"
                     /locus_tag="ACHK3A_003889"
     CDS             complement(12392..13069)
                     /gene="tli1"
                     /locus_tag="ACHK3A_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFO8741914.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKNLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWNDHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWNETYYRSTMIIGLA
                     PEGKVRVWLGDRGNPVVPQLDAKITTVSGDKMNMCKGVTKHPDGYVYYGDTPDFIKGK
                     IYPYGNW"
     gene            complement(13213..>13311)
                     /locus_tag="ACHK3A_003890"
     CDS             complement(13213..>13311)
                     /locus_tag="ACHK3A_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO8741915.1"
                     /translation="MCKGVTKSDFSYGYDEDIKEFIRDKTYPYGNW"
BASE COUNT         3370 a         3724 c         3044 g         3173 t
ORIGIN      
        1 ctttattttc cttctcagta actgaatatc tttgattaac gccctccgga aatatttata
       61 cccggaaggc tgtccagctt tatgataccc gccagtacag aattaactgc aggctgagca
      121 cagatttctc tgctatttca gtgtcagatt aatactgatt accggaacaa atgggcagat
      181 gtgtaaatcc aggtttgctg gccatataaa gcggcgcaca ggtgggttta cggggttcag
      241 tgaccggtac aacctcattt ttcccggcca ccgacggttg tgacaccttc cggacagccg
      301 ctgatttttt cttctgaacc agcttcgtgg tgaccggtga tttcacctga cgtttggcgg
      361 gtgccacgcc ataaccggaa caaaagcgct tcgactgact cagacttccg tcattgcaga
      421 caaaacgtcc gtctgacgtg cagtgtgaaa taccaccttt agcacccgag cagggttttc
      481 ttccccgcgc agcatctgtc tgaaccgaaa ataatctatg gtcgcccccg tttttgcaac
      541 actgattttt taattaatgg tgggcttgct taaatctatc cggcgtctct gtaggcaagg
      601 atgcctgcgc cccgatgagt ttcgcacctg ataatcctaa aaagcccctc ggcttcagtg
      661 agccatttta tttattaggt ttttaatcag gtcgatttgc cgttcatgtc atcaataatc
      721 agtcttcgca aaaccagaca ggcaactttg tatcttattg tttacaataa gccaattgag
      781 ttatatcagc atttatatca agcctgatat tctttttgct gagtagtgat tacccaggca
      841 attcgagcca gtttatttgc cagagcgcag gcaaccacat tcgaatgctt cttgttgagt
      901 tgctcccgaa cccattcggc aagttttcca tgctggcgtt caagccgcat catgaatgag
      961 cgggcacact gaaccagtaa ccggcgcaaa tttttatcgc ctcgcttact gatacccaac
     1021 agagtcgatt ttcctcccgt gctatactgc cggggaacga gccctgtgga agccgcaaaa
     1081 tctctgctgc atgaaaactg tttcccgtca ccaggttgcg acgatagtaa gctggcagta
     1141 atcggcccaa caccaggaat tgtcataatg cgctgagcgg tatcatcagc gttgattgac
     1201 tgacttaatt ctgattccag ctctgctatt tgctcaacaa ggtaaaggta gtgggtatgc
     1261 aacctcacca aaaggcggct cagatattcg gggatttcgt gttcagccaa cacgagagac
     1321 aaccgtttta ttacagcatc acctgtcggc aggctgattc caaattccag caagaaaccg
     1381 tggatctggt tggttgtttt caccttatct ctgatgagtg attctcggac ccggtgtaac
     1441 gccctcatgg cctgcagggg ctcagttctt ggctggacaa aacgcattga tggtcgtgaa
     1501 gccgcttcgc atatggcttc tgcatcaaca aaatcattct tattactttt gacaaacggg
     1561 cgaacgaact gtggagaaat tagcttagtt tcatgcccaa aatcagagat acgacgagcc
     1621 ataaagtgtg cacccgcaca ggcttccatt acaacaactg agggcggaca tgtcgccaga
     1681 aactgcatca gtttagtacg ggtaaatttt ttacgcagaa gagccttacc aaacttgtct
     1741 tgacaatgaa tgtggaagga gtgctttccg agatcgatac caataagtgt gatgttctgc
     1801 atgatggtct ctccgttaaa gggattcacc ttaacagcgt actgctgtta aggtgggggt
     1861 gaccatctca ttagtcccac gaacagaaaa cagataattt tcttcattta aaaactcata
     1921 cattgttatt gctgatatgc cagagttatc aggatgatga aacattccgc cagcgttgtc
     1981 ataagccgga ataatgtcac attcacacct gacatatatc catcactgtt tatttctgtt
     2041 ctgccctccc tgctccggct cacgtctgaa cgtaccgctg ctgtttcaga tggcgcaaca
     2101 acaccctgcc ctccggtata aatgttgttc cgtaacgaac cagcccggca tctttaagtt
     2161 ctgcatcaat gacataatgc aactcaccag gctgagtctg ctcctgcatc gtcacgttga
     2221 cacgcttaat acgtggctca tacttcagca gaacatctga aagcacacgc atcagttgat
     2281 gcgccgttcc cggcattccc tgcagaacgg ttgtcatatc cggcagacca taatccggca
     2341 gatgtttaag gcttccggcc cgggtattca gaatacgctg catgttatcg agcaccgaaa
     2401 gaataacctg ctcttccgca ctgacctggt ttaactccag cccgccagcg aaattgccat
     2461 agagaatttc ataaagggaa ggacgcggca tcactattta tccttcaccg gtaacagcgt
     2521 cagggtatta ccggatactt caataagacg gggcgtgtcc ggctcaagct catcccggct
     2581 cagcaccacc cgccaggtat ttttcttccg gtcaggctcc agaaacattg ctgccacacc
     2641 ggtaaatttc gccgcatcat ccagaggcat atccacggtt actgagccac ctggctgcat
     2701 ccatacatct ttctgtgcaa tgatatcgcc tgccagaatt tcactgtccc ccgtaaaaag
     2761 cgcctgataa tccgcgctgt caaaattccg gttgtcttta agctgataaa tccgcaccac
     2821 aaccgacagg ggaataccgg cagcactggt gttaatggcc tccctggcac gaatatcaag
     2881 gtgaagtgtt tttatctgtc ggtaaaacag ggatttcgtg gcagataccg taccgtctgc
     2941 cactctttgc gtcaggccac accctgataa cgacagggaa aacaacgcaa taatcagacc
     3001 gtaaccagcc ttaccagcga taatcgccat tttcctgagc ctccttacgt tgataatgtt
     3061 cctgaacccg ctgataacgc cccagacgga tggtcattac gcgtgccgct gaagtcgtta
     3121 tctttgcctg tgttctcatt acagccgtgc gccccagttg cggactaccg gctgccggtc
     3181 ggcaactgag ccggacatcc ggcaacagtg aacgctcaac acaaagttgc agacgtacat
     3241 ccagacggct tcccagataa acgtgcaaca acgccagcaa atcagaatac agctgcccgc
     3301 ccggtaacca gccctgtacc tcgctgcccg tctgtgttga aagctgtaat aacacctgtc
     3361 cgttcacatc cgtagcatga tcgcccatca ccgggcgact cttcagactg accggatgat
     3421 gaacacgcat ggccagcggt gttttcagcg ggatccgtcg tcggtcatga tgccagacac
     3481 gggcctgtgt tccgggagcc agcagcgtta ccagtgatgt cagcccttca gctgttcttc
     3541 cgggtaacaa catcaggggc aataacgcca gaaaacgcga taccggcgtg gcaatattct
     3601 gtgcacatcc ggggatcccc agtctggcca gaccgagcag atactgggat gttttatcct
     3661 gcccccccgc ctcaaaggtg gccgggtacg aatatttgcg ccagatacgg taatactggg
     3721 tgatcagacg atgactgaat atatcgagaa aatccgctgc ggcttcatgg ccttcccgct
     3781 gctgtgcaat atcactgata tagtgagccg gcaacggaga cgtgaccccg taaagtccca
     3841 taaacgtcac atgtacagtg ggtggcagat gggattcttc aggaattaca gcatctttta
     3901 tttcactcgc aggaaacccc attcccgtgt atggacaaaa gcgaaccgct tcctgacggg
     3961 cctgccagcc actgccaatg ggaggctgat ctggctggct ttgttccagt aactgacaga
     4021 agcgataaaa attaatatac ggaagctgtt tccggtaacg ggtaattaac cgggcaggcg
     4081 cggattgtga ttttctttcc accggatgca ttttccttca ggctgaacaa tcagtgtgag
     4141 ctgattgaac tgattcatat cggcgtacag ggccaggaaa cggttgagca tttcgccaaa
     4201 caggtgaata tctccctctc cggcaaaccc gttgccatca agagttacct ccacatcaag
     4261 cccccggagc agaaaaccct tttcaaaacg ctgaatacga tgatgctgta cagccagaat
     4321 ggcatcaagc cgacggtgat taagttcatc gtcccgccag ttataaagcg ccagcgtgcc
     4381 acgcagcact tccgcactgc tcagcatgtt aagaaatcct gtccccagat gactcatcac
     4441 ccgccagtga aaacgatctt ctgtcggcgg ataaaccggc agcgtgggtt tacaaagatt
     4501 acatacactg atcggggtct gcagtacctg ttcacaccga tcaagcagcg tactctgcaa
     4561 cgcacgacgg ggaagctgac cattggtacc tgtaatacgt aatgaaagcg tttcccgctc
     4621 cggcatccga tccgcctccc accgctgacc gccaaggata agccatgtgt catacatccc
     4681 cgtgacaccc cgcttcaccc gggtatgata ataacgttcg ggtgcatcat ggcgcagcat
     4741 tcccccacga tgacggaagc tggtaaaagg cacgtactct gcactgccgg ttcgcccgga
     4801 gcccgccacc gcatccactg aatatatctc cgtatatcca tcctgcagac gcttcgggcg
     4861 cagcagatat tcgctctcca gcccattgat gatgagtgga tcggcttcaa gcgtaaaaag
     4921 gttaatcacc ggcacacaat gcagacacag ggcatcatct gtcacaggta aatctgccgg
     4981 ccaggactga ctcagcacca cctcaatatc aaatccggaa atacctgccg gcaaagaaat
     5041 attctccagt ccgttcagat gaacaaacat aaatttttcc cgaaaggtga aatattccag
     5101 caataactga tacccactga aagcactgtc acctttcggc cacagccgat cttcttccgc
     5161 aaaaccaccg ggtgaaaacc atccgtcaat gcggatccgc tcatcatttc ccggcagcct
     5221 cagatacagc gcagccagac gctttgtcat catcagatga agggtgctgc tgaccggggc
     5281 ttctgctgcc agatacaggc tcagacgatg caaatcgacc tgagaccagt cagccagttc
     5341 actgcaggca aaacctattt tcagcactga gcgcccgtcc ggctccgtag tcataacgac
     5401 cttctccact gccagtggat tcagtggtat ggcccgggtg gtacggtaac gacagactgt
     5461 atttccgggg ccaacagggc gggaagtcac ttccagtccg gctggtacgg tttcagccat
     5521 tttcatgacg gataaccggg gagtcagtgc caccaccgac agggacggga tagtacgcag
     5581 gtaatgaggc cagagcatac tgaccagact ttcagtaagc tccggcaaat catcatcgat
     5641 tttctggcgc agccgtccca tggaaaaggc gaaaccttca aacaggcgtt ccacgcaggg
     5701 atcaggcgta cccgctttat cgagatccag catcgccgcc cgatcgggat gagcctgtgc
     5761 aaacgccttt ccggcttcac gcagataacg catttcagcg tcaaaataac gcagggttaa
     5821 atcgtccatt atcttctcag aataaagcta actgttccgg agactcgcgc tctgacttca
     5881 gccatatttc aaacaatcgc ccgaactgaa ctgcaggcag gatttcatta atcagattac
     5941 acatgaatat aattcagaca tctcatgaac ttcccgaatt gcacaacaca gcagctctgg
     6001 cagcatcaat ggcgatgagc ccggcaagaa gatgttccat atcaggctgc agacgcaccc
     6061 tgtcactttc agtttttgcg gacttcatac gtaacagttt caggcggcgg gcttttacct
     6121 caaataccag ttcaggttcc cattgtgaca gggtcagccg ggttgcacgt tcatccagct
     6181 ccacaagcag atgcagggca aggtcatttt ttccagtctg ttcggcaaca cgggccatca
     6241 acaggcgcag tagccagttg ctgcggtcag actgaatacc gggacgtgcc tgtaaccagc
     6301 ttaaagcggc ttctgttcct tcattatccg caatctgcag ggcttccggc tcaagagata
     6361 aaatgtcgtt atctccggga gctgccgtta tcgttacaga aggttcttcc gcccagcgtt
     6421 caccacactc catcacctgc tgctgtatcc agctcagcgt cacgtcatcg gcaaacggca
     6481 tgccgtcgtt aaaagccagt gtctcaagcc ccggcaggcg cagaagcaat cctttcaggt
     6541 catactgaat aatggcagca taattttctt ttccggtctg caacagcgcc tgatgaatat
     6601 accactgcag gtccagccac agatgactgg ctccccgggc aaacatatcg tcacactgtt
     6661 caagcagtga caaccagttc tgctgcagat acaaccgttt cagtgacgca cgccggtccg
     6721 gccccggtgg ggcaatacgg gtccgtccat cagcagataa cggcggtaac tgatgcagcg
     6781 tgtcatgccg gacgcttttc atcagtcgat gagccgccag ccagccctca ggctggtcac
     6841 gcagatattt tgccagaaca cgggcctgtg acagcagttc ctgtccggag gtgatggcag
     6901 ataatgccat gacgctgccg gatgttaccg tcggtgccgg cgcggcagcc tcctgaggca
     6961 ccactgcatc aacaccaccg gccttcatca ggcgattttc aagtgcctga tataacccgt
     7021 tcagcaccgg ggcggaagcc tcatccgtaa aggtctgttc tgccagccac agcgccccgg
     7081 caatgcgcag ggtatccgcc ttcaccacct ccggatacag agacagactg tccagtatgc
     7141 gggcgctgca taaccactcc agcgccgcct tcctggcccg tgaccgttgt ggatgcaggt
     7201 gctctccaaa acgctgcaac aaaccggtca gcagctccag accgtcggcc agcccttttt
     7261 caccatcctg ccgcagacgg gcccagatat accaggtcgc cacccggata tttttggttc
     7321 tggtggtcag taatttttct gcaagctggc tgacaattcc ggcatcggct ccggagagtt
     7381 tattgacctc ttcacgcatc tgctgaaaat catcatcgta ccccgcatca tcacctgcgg
     7441 gattatctgg cgtcacggga gcaagccatt tatcccacag cgcaatacgc acctgagccg
     7501 gttcagccac atcccggggt gtcgtctggc atgcactgag gagcgaatga atggaagcca
     7561 tcagtcagtc tcctccacgg aatccccggc atcttcgtca ttgcctgtaa acgctgacgt
     7621 accggagagt tcgaaaaccg tttccggcag aacaaaattg cggagtttca gcaatacaag
     7681 cggcccttcc cccgcttcag ttcgcagtgt gtaattcagc atacgaccgt cctgcgcctg
     7741 ccagctcagg ctccagccac tggcaacgcc cggtgcggct ttccgtcggg ccatctcaag
     7801 cagacgtatc agcccccagc ttcccggcag gtctgcatac tgacgggtac cggcctgagt
     7861 gcttacccag cttaaactgg cgccaggtgc ttccgtatca gccggccagg taaatcgctt
     7921 ccataccggc atctgattaa cataaatcag tttctgatta tcggttatca gcgtcgtctg
     7981 catcacaccg gcagcagttc ccgggcgcag ctcaaaatgc agccccgcgt tacctgtggt
     8041 gaacgccaca tccgctattt cactcagggt attgatggct ttcagaaacg cagggttgaa
     8101 tgtcagtccc cgggtattaa tggtatcagg tacccagcga ctgccttcac tgtgcaacac
     8161 accactcagg ttattctgca ggaaacgggc aatacgtccc gtgtccgtat tcaggtattt
     8221 tgccagcagg ggaagcgaag catcgctgct gacattttta aacggataac gcccggaaaa
     8281 tgcattattc cagccatcca ccaccgcagt acgccaccgg gcattcagac tttctgccgc
     8341 gggcgtaaga acctgctgcc acgcctgctc catcgggcga acaaagaccg tctgtccgaa
     8401 gccataccac tcctgtccga gtccggcagc ggtcaggcta ccgtaatccc gggtatcggt
     8461 taaatcaacc gatttcccct gcaatactgt ctgtgccagc atctgcatca tggcctgcgg
     8521 gtcagaagag ccggcaatct gttgcagacg cagacgaacc tgtgtcaccc gggtcagata
     8581 tgtctgcaga ttcagcatat ccgcactgtt gttctgatta tccatcagtg caagcaccgg
     8641 cccgaaagtc gtcgccagag gaccgtgaag ccggctttcc ggtactgcca ctggctgttt
     8701 ttcctgtgaa agcaggtttc tggcagattt aaccagtgaa tcagtaaccg cttcccgggg
     8761 ctggccagta cgcccctgaa ccgccagcgt attcatcaga gcaaccaggg gggactggcg
     8821 cacatcagcc atcagcgtca gttgctctgt cacatcagac agcgtctgag ctttacgcaa
     8881 gtgcagactg ttcagaaaat tcagccaggc attaccaaaa tcagcaaaat agcgcgttgt
     8941 caggcgctgt ctgagtgctt ccggcgatac cggtgacggt gccttctgcc tgccatccgt
     9001 cagtacccag tccatttctt cacgacgctc attaatcacg gtgtctatcg acggcaatac
     9061 ggcctcttcc caggcctggc gggtaaacat accgggaacc acttcatccg taaaaaagag
     9121 gcgggagaca tcagtatcac cggtcatatc cgtcagacgc atatccgcaa actgatgtgc
     9181 cacccgtgcc agcattttct gatacagcat ggcatcgctg ttctgtgttc ccagatggcg
     9241 aagcagcaga gttctgctct ggctgaccag ctcttcatca gccgtcagct tccactgagg
     9301 atgagtaata atcccggagg cataatacgc cagcaacgtc gggccattag cctgccagaa
     9361 aacggcactc acaccgtcac gctgtggcca ttcccgcatg agcgtacgac tgaacctggc
     9421 gggctccata tgttgcggtt gcgccagcat cagatacagt ctgagctggt catatgcggc
     9481 ttttgccatt ttcccccgct gcgggctgtc cggaggaaga cgaataaatg tcctgagctg
     9541 ctgctcaaga tgagcagcag cggcatcacg cagtaaaaga tgtgcatttt gtgcataaag
     9601 gggcattacg accgcgagca ggtctgcatt ctgattcata ccggcccgca ggtaccatgg
     9661 cgccccgtgc tcctcccggt actgaaggcg ggccagggat ttctgcagat tcagcaacgc
     9721 ctgcagacgt tctgcaggag acagttgctg gttctgagcc ggacgaatct gttcctgtac
     9781 ttcagcaacc agactccggt ttgccagaaa ggaaaccacc attcctgttc cctgagccat
     9841 catcagtatg gccgccataa cggccagact tctgcgccag ttatgacctg tgcgggaagg
     9901 ctgtagccgg acaggtaatt gctgcactgt ctccggcaac gcctcccagc ggttatccat
     9961 tcgccagcga tgtctgacac ttcgttcacc gccgacagtt gccggactga aaacaatacc
    10021 cgccagcaac aactgacggt atggacggag taatacggat aatggctcca ccacagcatc
    10081 aatatttcgt ctgaatcgct ctgccagcga caacaaaaaa taataccgag gggcacagca
    10141 tatctgctgt atcccctgag caacaagccc ggggagcatg gcctgtagtt gtgctttcag
    10201 tttgtcagaa ctacattctg ctggcagaag gcatcccacg ggctgagtaa tacgccctcg
    10261 ctcatccgga ctctcctgca gtgaccagac atacagcggc agacgccagc caaggcgctc
    10321 acaacatgaa ctgaccgcac gggaaaccct gtcaagggca tcttctgtca gtggtgcgga
    10381 taatgtttca ctcagtccgg aagttaccca gacaatgcca tcagcaggcc ggtaacgcag
    10441 acggcgtaac gcagccagcc agtctgcgtt ttccggctgc gaggggtctc ctccccagag
    10501 aagcaatgtc ccctgctctt cctgccagaa ctgttctgtc aggcccgggg tcagcaactc
    10561 aacctcagag gcagtacctg ttatcaacag aatgcgtatt ttacgtcccc agaatctgcc
    10621 atgctggcgc cgcagagtat gacgaatatc ctgaacagta acatagcgcg gtgacgtctg
    10681 agccagtcgc tcttcatcgt caggtaacct ttgctcctta cgggcccgac gtcgatatgt
    10741 cagttcctga acatattttt tcatcacagg caggtaacag acaaagagca accccagact
    10801 cagcaatatc agcagtagta tttgatcccg gcgtgtttct gcccccagaa atgctccata
    10861 tcgggttatc agccaccaga gcgcagccac accaataaat acaatccccg gtcgtccaaa
    10921 gcgaccagac agacaggcca gtttattcat tctggtttcc tgatgtaaca gtactgataa
    10981 aatgttcttc tccggatgag aataaagtca ggagagaatc attaccaaaa cagaccagat
    11041 cggctgccag agcagtaagc agccacggag cagcaggtcc cgggataccg caccattttt
    11101 ccagtaacat acgtccggac ggttcccatc cggcaccata ctggtttcca atagtcatca
    11161 gcggtgcagc aatcttttcc cagtgataac agtcccccag tacacgagcg gtacgacagg
    11221 cagctgtctg ggtttcaagg aaaagtgtga attcatcatt aaacttgttg atatccagcg
    11281 acatcgggcg tagcagacgg gctggatgcg gcaggttata tttttgcgca acatcatcgc
    11341 tggtcaacaa aagtgcagcc agtccgtcag agtatgcatt tcccccatta agctgtatta
    11401 ccagtatcaa atccacagtc agcactggtt gtttcagacg ctcatcaacc caccccatgg
    11461 aaaagtcggg agtaacctcg atattgtcag gaacaacacg ttggggaaat aacacttccc
    11521 atgcagaaac aaaagcagat ctgatttcag aatcaggctg atcggttatc agcgtcacat
    11581 ttacagggag aacggcaggt aactgacaaa atttatccat tatctcccta agcaagacac
    11641 gtaaagaggc ttcaacctta tgcccactgt cagaaagata atctattttc tttacaaggc
    11701 cgtactgcag aggcaattca tcacaaagag tagctaccgt tattttatca ggcaaataaa
    11761 cacaactggc agtaataaca agataccttt cggcccagga ttcccactgt ttctgagcat
    11821 actccccctc ttttttaaag aacaaatgtt catccatctg gttgctccac tgccagtgtc
    11881 gcaggcaaaa caggaaaaac cagccccccg gaggagtcaa tgcaagccac cagatactga
    11941 aatcatttaa tgctttcaca tattctgatg cccggataat aaacaacaga gcaccaacaa
    12001 cagccataaa tgcccctgtc ataaaccaca acatcattga tggtttatga ggtaattctg
    12061 ttgataatga ttttgttctg ctccatccca tgggttatcc tattgcacat tcaggtgcac
    12121 tggatataac cttacaccca cacagacatt tacatccatc aatcacaaca gctcttccat
    12181 cagatatcca ttcaggagag ccctccacaa taggattagt accatgtccg gaaacaggac
    12241 aactaaccat atctccgaca agagctactt ttttaccatc gacaatcatg gttgaagatg
    12301 ccgaaataac tttaccacca tgtgtggtat tatcaccaag caatacaaaa cctttagaca
    12361 taaaactctc aaaaataaat tttactttga gtcaccagtt accgtagggg taaattttcc
    12421 ctttgataaa atctggtgta tcaccgtaat aaacatatcc atctggatgt ttagtaaccc
    12481 ccttacacat attcatttta tcacccgata ctgtagtaat tttagcatcc aactgaggta
    12541 ccaccggatt accgcgatct cccagccaga ctcttacctt cccttcaggt gccagaccaa
    12601 taatcatcgt tgaacgataa taggtttcgt tccagcggtt gtctgtgtaa ggcgtaacca
    12661 tctgctccca ggtccctttt ttaaaaaaaa tcagagtttc ataactcttt ttatcaatta
    12721 ttgaatccca gcaaaaacgt aacatcagag gtaatgcttt ccccttgtta taatacgact
    12781 cagtcgcgcc cagatggtca ttccagcgcc ctacactgac ctgactcggt cttgtggtat
    12841 caagatactg ataagtcgtc ttataacctt ccccgtccac catcaatgcc tgagtggcca
    12901 gcgcaggcag agcctttgga taaataaaga gaaatttcca ttcatcatag ggtaaatttt
    12961 tataacccca ttcagtctta tccgaatcag catactgtga gctaaccgaa ttacacccag
    13021 ttaacaatgc cagtaatggt gcgataaaaa tgattttatc tctggtcatg tattgtcatc
    13081 ctgtagcgtt aaacaccaga catcataact ttagtatttt ttactgcgtt atcattcagc
    13141 tattgaccgt ttttttacgc cagatgaaaa ttagactttc tttttacata ctcatcatta
    13201 ttctggctta agtcaccagt taccgtaagg ataagtttta tctctgataa actccttaat
    13261 atcttcatca tatccatatg agaaatcact tttagtaact cccttacaca t
//
LOCUS       DAZGHF010000126        13308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGHF010000126 DAZGHF010000000
VERSION     DAZGHF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.127"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(155..1576)
                     /locus_tag="ACHK3A_003891"
     CDS             complement(155..1576)
                     /locus_tag="ACHK3A_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO8741916.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNNSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAMADTVETALLMRRMLLTG
                     MAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLARNSVLTIIERDNERVGN
                     NPMIQQADNADSRIHSLDAPDKE"
     gene            complement(1590..2564)
                     /locus_tag="ACHK3A_003892"
     CDS             complement(1590..2564)
                     /locus_tag="ACHK3A_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO8741917.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWLFCTKFSCKVRTSVKVSHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPE
                     NAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESP
                     VTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQ
                     TDDDKAAAVVAQRVADIITRTGEPHVYQPVKAGKKDGYWPPDPVEENTGTRNHKWQRL
                     TPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            complement(2561..2956)
                     /locus_tag="ACHK3A_003893"
     CDS             complement(2561..2956)
                     /locus_tag="ACHK3A_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFO8741918.1"
                     /translation="MYRLLLYSFLILPVAASAGTTIYTDSHQRPMNPPAGVRVVLLDA
                     PEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYP
                     AVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            complement(3092..3724)
                     /locus_tag="ACHK3A_003894"
     CDS             complement(3092..3724)
                     /locus_tag="ACHK3A_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8741919.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSSVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
     gene            complement(3916..4194)
                     /locus_tag="ACHK3A_003895"
     CDS             complement(3916..4194)
                     /locus_tag="ACHK3A_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741920.1"
                     /translation="MYAEKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQL
                     SDVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDTAPEKGAEISVTEE"
     gene            complement(4574..4762)
                     /locus_tag="ACHK3A_003896"
     CDS             complement(4574..4762)
                     /locus_tag="ACHK3A_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO8741921.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQDECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            complement(5140..5517)
                     /locus_tag="ACHK3A_003897"
     CDS             complement(5140..5517)
                     /locus_tag="ACHK3A_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO8741922.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            complement(5522..8218)
                     /locus_tag="ACHK3A_003898"
     CDS             complement(5522..8218)
                     /locus_tag="ACHK3A_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO8741923.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRVREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLAGRE"
     gene            complement(8356..8748)
                     /locus_tag="ACHK3A_003899"
     CDS             complement(8356..8748)
                     /locus_tag="ACHK3A_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO8741924.1"
                     /translation="MTVMVAVFILPGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            complement(8784..10271)
                     /locus_tag="ACHK3A_003900"
     CDS             complement(8784..10271)
                     /locus_tag="ACHK3A_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO8741925.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEQQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            complement(10261..11169)
                     /locus_tag="ACHK3A_003901"
     CDS             complement(10261..11169)
                     /locus_tag="ACHK3A_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO8741926.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            complement(11169..11831)
                     /locus_tag="ACHK3A_003902"
     CDS             complement(11169..11831)
                     /locus_tag="ACHK3A_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO8741927.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            complement(11828..12190)
                     /locus_tag="ACHK3A_003903"
     CDS             complement(11828..12190)
                     /locus_tag="ACHK3A_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO8741928.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGGIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            complement(12203..12517)
                     /locus_tag="ACHK3A_003904"
     CDS             complement(12203..12517)
                     /locus_tag="ACHK3A_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO8741929.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            complement(12594..12836)
                     /locus_tag="ACHK3A_003905"
     CDS             complement(12594..12836)
                     /locus_tag="ACHK3A_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO8741930.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            complement(12836..13177)
                     /locus_tag="ACHK3A_003906"
     CDS             complement(12836..13177)
                     /locus_tag="ACHK3A_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO8741931.1"
                     /translation="MFSFPASPCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
BASE COUNT         2983 a         4263 c         3095 g         2967 t
ORIGIN      
        1 catccactcc cgcagggccg caaatccttc tgcgtgacgt ataccggtat cggccagcca
       61 gagggaaaac acggtaatcc ctgtcagcaa cagcgtatac agggcgaata ccttcatcct
      121 ctttttcacc cggccataaa ttctgctcat cctctcactc cttatccggg gcatcgagag
      181 aatggatacg gctgtcagca ttgtcagcct gctggatcat cgggttattc cccacacgct
      241 cgttatcccg ttcaatgatg gtcagaacac tgttacgcgc cagatcccgc ttcagctcca
      301 tctcgttttt cagcgccata atttcccgat ccagcgcctc aatacggcgt tccccctctt
      361 ccagtgcagc aggctgagct gcagcatacg gctcggccat ccccgtgagc agcatcctgc
      421 gcatcagcaa cgccgtttca acggtatccg ccatggccag ttctcccgcc agacgcgacg
      481 tcagggcacc attgtccgga tcacgctgca gggcacggat cactcccgct gtcacagaca
      541 gactcccggc tttcagtttt gccagattcc cggctgtcgg cttgtcacga ccattgacca
      601 tacctgccag cagttccgtg ttaacccgcg tcgcttcttc aagcatcggc gcaaaccccg
      661 tgccggcaat actggtcccc ggctgctgat cagcatcccc gctggtacat gcctttgcat
      721 caccacaggt ccgcatggat ttatccccca gaaccttcac caccgcccgg gcagcttcct
      781 ctgcattacg gtatttcatg caggcactcc cgttacagtt cccgggactg acactggatg
      841 tactggtcga aggcagactg ttcatcatat tgaaaccggc agctgtcaga tcatgtgtcg
      901 gccggatagc agactgtccc gctccaccac gctgctgccc tccgatccag cgttgcccct
      961 cctccccggt aacctgacga cctgcttcat cccctttgat cgcatcaccg tcccccgtat
     1021 tcacaatttt tttgtactcc tcgagcatgg ccgcctgcgt ccacttattg ctgtccgtga
     1081 actccatcat gcgtttcgcc atgttctgac aattgagctg cgctttgtcg aatgccacat
     1141 tcgcctgcag tacaccgttg gtcagcatgt catacagccc cggattagcc cgctgtatca
     1201 ccattgccgg cagactcgcc acggctccgc tcgccccctg aatcacatcc cccatcaggt
     1261 ttttgaaacc atcagtgatc ccgttcagct ggttacccac tgttgttttc aggtcaaaat
     1321 tgccacacat cagatcactg ctccacccga gatccacccc aagtttctgc atattgctgc
     1381 gcgttgccgg ctgcgatatc accgagcccc ctcccagggt ataaaacagt ttgtctgata
     1441 ctgcgccgtc cacggattta ccgtagccaa acgcactgtt attcactttc ggcagggaca
     1501 aaaagtttcc gtcagcctgt accgtacccg cacaaagcac cacagccaga acaggtatcg
     1561 tcattttctg tttcatcact tccccttact taaaaattcg tactgcccag gaatgtctgt
     1621 ccccgtttct tgcagcaact gtagggctgc cacagggcaa aggcagcatt acctgtttca
     1681 gccgcttcat gctctccgtc agggaagact gcgcaggacg tggagactga aggcgtcagt
     1741 ctctgccatt tatggttccg ggtgcccgta ttttcctcca ccggatccgg tggccagtaa
     1801 ccgtccttct tccctgcttt caccggctga tagacatgtg gttcaccggt gcgggtgata
     1861 atgtcagcca cccgctgggc caccaccgca gcagctttat catcatctgt ctgggtcacg
     1921 aacccggagc ggggatacac attgccccac atattctctt cttcctggct gcctatttcg
     1981 cgtttaccgg ggatcagggc ctccgggtac agtgactccg gtagtcctgt acgccaggca
     2041 atactgtcaa gcgtactgag aaaataagga acaaaaggtg tcaccggtga ctcacaggaa
     2101 tatcccggca ccatccctcc gataagcgtg gtggccgggt gccctgttgc atcggcatat
     2161 ttaaaacgga ttgacgaacg acgttccccc tgtaccttca tgtctgcggg gttccctcct
     2221 cctgctgaca gaccggcaat acctttgctt atggcattct ccggaccacc ggctgtactg
     2281 ctgaccgccg acatttcacg ccagggattt ccgccaggtg cggtataggt ggaaacaacc
     2341 acttccggga tataatggga aactttcact gatgttctga ccttgcaact gaatttggtg
     2401 cagaacagcc agtagcaaat cccgctgacc cgccagctga tacagctctg tgaggctgca
     2461 ctggccacta tctgcgcggt gttcagcgat gcctctgcca ccggcaatac cgccatactt
     2521 aacagcagaa caccggcgcg atgacgtgat gtgcaggaaa tcatggctgc cctccccctg
     2581 tacgcagttg ttctgccagc accacgtctg tggttccgta gaccacttca ctgtcatcaa
     2641 aaaccacagc cggatatttt ttcagtccaa gggaccaggc atgtgctaat gcacggtagt
     2701 gttcagcaag ccctgcctga aacgactgcc attcaggcga ctgcatccgt tccatgacac
     2761 gggcagatgc ttctgcaggc tctgcgggaa gtaccccgaa aaatgtatcc tgcgtctgct
     2821 caggagcatc aagcaggaca acacgaacac ctgctggcgg gttcatgggg cgctggtgcg
     2881 aatcggtata aattgttgta ccggcagatg cagccaccgg tagtatcagg aaggaataca
     2941 gtaacagtcg gtacatgatg cgactccgtc agacaactgg tatctgacag cgtgcgcaaa
     3001 ggcaaaggga caatcagcaa taaattcaaa accggatgat aaaaattaag cctcttcctc
     3061 tgaataacgc acactctgca tctgaatcaa attaatccgg ttgcatcaca ggaagccact
     3121 gattcggggt aataccataa taacgcctga acacagaaat aaaataagaa gcactggcat
     3181 agccacacaa ctctgccacc cgggaaatac ggttttctcc cgcagacaaa agctgagcgg
     3241 catactgcat tctctctttc agaagaagct cactgaaggt cgtcagctct tcctgaagct
     3301 ttttcttcag taaactctca cttatatata acagactggc cacttcaccc agcatccatg
     3361 gacgggacaa atcagcactg ataatattct tcacccggac agtgcagctc cggatacacc
     3421 ccgtgagcag ggcgtggaaa tccggatgtt cctcaaacag cgccagactg gaaaaagcca
     3481 ccgcatccct gaagtacacc gacgatggtt catctgtaat aagttgtctg atcagacatt
     3541 ccgccagcac ggacatctgt ggccggatct gtaaaagcaa agacgacggg ctgactgcca
     3601 taccggataa tggcgtgcag aaccgcagat accgcctcac cacctcctca ctgaggtcag
     3661 cgataaggaa acgtccggta tgagcctgaa aagcagggcg cagatgcccg gcaatcagaa
     3721 gcattgtgcc ggcaggtatg acagagttcc tgtgctccag acaaagcatt gtctgtttga
     3781 gagccataat gacaacgcag gtacagggca tcggatatcc ggtaaatatg ttgatttatt
     3841 aaagagacag acatgccccg tatataaaat tccgtgtcac cgggtgctga aaaaaactgc
     3901 accgacggcg ctcttttact cctccgttac ggatatttca gccccctttt caggagcggt
     3961 atccttcacc tcgctgagcc cggactgagc cgcttcagaa acctctgcca cctttccggc
     4021 agcctgcatt ttcacttcct ccgcggcatc cttcacatca ctgagttgag tctgcgcagc
     4081 ttcctttata tctgccgcct tcttacttac attctccttt gtcgcatcat cacagcccgc
     4141 aaccagaaat ggtgcggaaa tacataacgc cagaattgtt ttttcagcat acaatctctc
     4201 attattaagt gttaacgcag aacagcttat atttcgtccc cgggaaatgg tcaaaagaca
     4261 accagaacat cacagtgtca gggcacactc ccggtatatg cttttataac cataacatgc
     4321 tgttcctgat attaccggaa atgtgcccac tacatttaac ctgttttcag caccgggtca
     4381 tcggctgtgc tgtatttttt attcccgtca cagaaacaca ctattaccgt tgatttcatg
     4441 acgaataaaa tgaataaatt ctcccgtggt ctttaccacc attattatac caataacacc
     4501 atttacacag atacactccg ggactatctg tttttgcccc ctccgactcc gccggaggtt
     4561 tttattttca tactcaggcc ttcaccactt cacaccggaa gtcctccacc ctgacgccaa
     4621 tataccgtcc cggctctttt gcgggcgcaa acagtttttc acattcatcc tgagtcattt
     4681 tttcatcctg gtaacgcagc cactgaaccg gcatattgcc tggtttgatt attgtcgcag
     4741 ttattttata tcgctttccc atactggctc tcatctcaac aacaaaatca ccggctgagc
     4801 catcactccg gtaatgcatg cgaaaaaaag tgagcgtctc agcaatggct catgcggtga
     4861 ttctgtacgg tacccgcaca aaatacgggc accaacagcg tcgcagaata caacgaaggc
     4921 atcagggcag cacctctctc ccgctatttt tcagagttct ttaccctcct cctgacacgg
     4981 gagtacaacc ctgacagcat ccgggccagt acattcgcaa gcccccatga aaacacagcc
     5041 acacccacag ctcccgccag aaacagcaca ggttcactgg caatttgatc tgacagcgat
     5101 ggatacagtg gtcccggaaa atcgatatcg ctcctcatat cacccctctc ccagccacaa
     5161 cagcagatag aacaaatccg ccgcatcagt cactgatata taccagcggg gatgaatata
     5221 ctcctcctcc gacggccagc tctcatgccc actcacagta agcagaatat gcacatactg
     5281 accctgctga ccttccagca tcaccgccac actgctgttc agacccaccc tctgtggctg
     5341 gcaacggata cactccaccc gccccagttg cgccgccaga tgcccgcgaa gacgttcaag
     5401 attatcccgt gtggtgcaac ttatccccgg cgcaccctgt ggcggcagaa caaacacact
     5461 tccggcgggg tgaacagcag acaccacttc cggttccgga tacttcagac acagcatagt
     5521 ctcactccct gccagccaga ccacgtagcc cgtccagctt tcgtgccaca tgaaacgctg
     5581 cttccagttc gctgcactgg tgcaccttca tcagggcgcg gcgttcagcc ttctcttctt
     5641 tctccgtcat ccccagagcc agatacagac tcggtggcac aacccggaac aacgcttctg
     5701 tgcgcttcgc cagtaccacc ccctccgtat aacagccgga cagtttactg gctgacagca
     5761 gtaccgcctt ctgctcctcc gtcagggatt taaaacgggc aatattgttc acttcctccg
     5821 gcggcatggt caggcacacc caccactctg ccatattcag catcttctcc gagacatccg
     5881 gataatcctt caggttctgc gtggccagcc acagccaggc ccccagcttg cgccacattt
     5941 tcaccacttt ggtcatatac ggggacaaaa gcgggtttac cgtcacgata tgcgcctcat
     6001 ccaccacaaa cagaatatcc cgtcccagat actgatcgcg ctcggcaatg ttgttaatca
     6061 tattagtcat tgacaccatg gtcagtgcca tctgggcttc ataaccttca cgggcaagat
     6121 gtcccagatc aatcagggtc acatccgctt ccggccacag ttttccttcc cggttaaaca
     6181 gctccgcttc aaaactgccc ggctgggtga acatccccag cgactctgcc atctcagcag
     6241 ctttagcccg gcgaacatca ttacgctgag tgtcccgcga aatttcccac aacgcactct
     6301 gcagatccac cggcagcatc tgacgcccct cccggtacgt ggtgtgcgtg gccatcagca
     6361 gtgcctcacg gatcatcgcc ctgtccgcac gtttgagcgc ggcttcttct ttcggatccc
     6421 ctccggtgat catcatgcgg gcagagattt ccatctcccc cagaatgtcg cgtttctctt
     6481 cctcttcatc cccctcatct tcatcaagat cgggcagtga atgctcatcc acactctgca
     6541 gcgtctctcc ctgctccacc agttgatgag catccgcaaa tggaggaaga cacacccccg
     6601 ttcccggttt gacactgatt ttattaaccg aaagcccgag actctcaaaa taatccgcca
     6661 gcagaccaaa cgagttcccg gcctcagcga taaacagacg gggacgatga actgccatta
     6721 tctgcgacaa cgaaccacac agcgtggccg atttaccggc tccggtaggc ccgaaaagca
     6781 gcagatgtgc attctgggtc cggtccagct tgttcagcgg atcaaaggtc agcacatccc
     6841 cgccacggtt aaagaaactg aatccgggat gccccgtacc ggtttccctt cccgtcaccg
     6901 gcaggagccc tgccagatgc tgtacccaca tcagacgcgt gtaccactgt tttttatccg
     6961 tatcaggatc aaaacacatg ggcagcgcgc gcagccagct gttcagcggt ccgacatcaa
     7021 actccgggcg cactggctgt aagcccgcgc ccagcagcac cgtcgtcagt tcaacccgtc
     7081 tgcgcttcag actctccata tcctctgccc gcagcagaaa cgtgatcccg gcacggtaga
     7141 gcttgtgccg gtttcccaga tatttcttca cggtcgccac atcctcccgg acacgcaggg
     7201 attcactgtt ctccccgacc gcatttttcg acaacctcgt gaagcgctct tccagtgtgt
     7261 cctgtggctg gaccacaatt gtcatacaca gcaccgttcc ctccggaaag gtgtccatca
     7321 gggcattcac tgtcttttcg ccccgggcct gttcccctgt gatggttccc ggctccggtg
     7381 gtgtgcgcag tttctccacc acaacagcgg catgcggaag cccgtccagc caccacacgc
     7441 cgttttccgg atcagatacc ggtggggtga accagaggct ttctgcaaaa tcggtcatca
     7501 ccggcatcat tccctccggt gtctcctgcg gttctgcacg ggatgccata cggtacagaa
     7561 tatccctgtc cacccattcc ggccggggat tgaacagcct cagcagccag ccatgcacct
     7621 gctgcccgtt catccgtgta cagcggacgc ctgcgccacc cagcgccccc acgacccgcg
     7681 aacagacctg gttcagcatt gccaccggcg gcatcggatc gtggttgttt ttcccgatcc
     7741 accggtaaat caccatcctc gtgcgccgct gctgaccacg ccatggctga cctgtgacca
     7801 gcgtatccct gaacagacct tccggtcggg caatcccctt cagatggcgc tccatttccc
     7861 tcagccaggc ctccgtaaat gaggagcctt ctgcatgagg tttcacgtac cccctgagct
     7921 ggtcaacata cgtgtccacg tcgttttcat cctgacagaa gaactgcacc acccagggat
     7981 gtgagtcata ttcatcaaaa ctgtcctgca gcgcatcctc caccgtatcc cggatttgct
     8041 ccagacgctc tgcagtccgc ccttccgtag cggccggact cacctcatac acggcaccaa
     8101 ccgacacccc atcatccagc agcaggcact gctcttcgtc cagatattcc acccatggca
     8161 gaaaatcaat caccgacgga caggcatggt aaaccttcgt ctcatcctgt cgggtcatct
     8221 tccccggcct tgacagtgcc tttcgtacct gaggggccac tctttctttc atatccggag
     8281 ataccgcttc tccgtcatgc tgcgacaccg gggaagaaac ccgttttttt gtgtctgtaa
     8341 acagggaaaa tcgcatcaca acgcctccgt tcgttcaccc ggcatggcat actgcgtccg
     8401 gctgtaaaaa ggaaatactg tgctgtaccc cggaaccggt gacgtaccgg aagccagatg
     8461 cggaaaaaca tacatcacca tgtcaggatt gggcagccgc ggaaactgct gactgatctc
     8521 actttcctgt gtccggctgt agcttatggc tccgtgcgca tctgcctgct gctccggcat
     8581 tgtcagcgga cgccgcaggg tgtccctggc cgctgtcatg tgccgcgaat gtcctccgct
     8641 tcctttcccc tgccagatct ccagcatact ctgttcaccc ggaggcagaa gctcctcttt
     8701 cgatgtcgag cagccgggca gaataaatac cgccaccatg acggtcatta cagataactg
     8761 tcgtttcacg ggatctcctt acatcagtcc agaccaccgg taccgtggac ttcccccggc
     8821 agagtgaagt cgtaacgtac ccggcgcccc tgatcttcat aatcaatggc cagttgacgc
     8881 gtgatatgca ccgccagcgt ctgccccggg ggaacataaa tggcatcaaa cgtctgcccg
     8941 tatcgctgct tcacccattc cgcggtttca ttcatcccgc cggacagggc ttttccaagt
     9001 gccgcctctc cggcactgcc ggtcagtgtt gatgtgatgc cattgacgtt gttctgtgag
     9061 gtgtactgcc cctcactcag tgcatcaccc gcagcgccgg ctgcagacag ggcaaatatt
     9121 gtcggcagat acgtggaggc attggattta cgggttccgc tgatacacgg gatcccgtca
     9181 tcatccgaaa tccagccaat gcctcctgta ctgctgttat tttttgcctg tgcgttactg
     9241 ctgccgttca cgtctgatga cggcagcgta cgcactgtcc cgtcggcaaa aacaaacgtg
     9301 atgctgttca cctgcccacg gacacaggag agcgtccagt caccactcgc agtaccggac
     9361 accactgcac cttccacatc cggcagctct attccgtttg ccgtcaggtt atccttaccc
     9421 accaggatct tgaacggata gggatccgtc acggttccgt tcaccggaac ccgtcccaga
     9481 agcgctgtca tcgcccggct gcctgccagc gtcgagttct ccggcagggt atacactgcc
     9541 cgggtgctcg ccttctgctg ttcattgctg tatcccttca cctgctgttc ataagccgct
     9601 ttctgacggg tcagtggatt gtcatccaga aaagagactg gaaaacgcgg tccgctctta
     9661 tcccccaccg acgacgcttc atcctgcacc tgatcggccg gtgtcaccca catcagacca
     9721 tccgttcccg gtgaggctgc cgatcctgtc acgctcccca gtccgtcaag gcccagacct
     9781 gctggaatat cgctctcctg tcctccggcg ccggcatcag cactctcctg ctgacgcttc
     9841 agttgctctg tcagtgactg aacctgtgtc attaacgcgt tctgttcatt ccggaactgg
     9901 cgctgcgact gctcataatt tttctgcaat cctgccactg cctcactgac ctgaccggaa
     9961 acatccgtgc cccgcttgcg cagcatttca ttttctttca gcaacttttc attctgtttg
    10021 tccagtacca gttgccgctc cctcacctga ttcatacgcc caatcagtgt gcgcagggta
    10081 tcttcaggtg tgtctccttc cacgcccagg gccttcagtt catccggtga cagtccggcc
    10141 agcgttcctg agctttccgc agacgagggc tccccggtat ctcctccaca gctttttaat
    10201 cccgtcagga tccccgccac caccacacag ggcaccacat acttcacgat cgtattggat
    10261 ttaatctgca tgcttcacct ccccccgtga aggaatgcga cgggaggcag gctccgcgat
    10321 aaacgcctgt tccggccgtt ctgccgtcac cacatagagc gtggtggtat cctcaggggt
    10381 acctgctgca cccagccagc ggtgctggaa agtcgcggca atgaattttc cctgaagtgc
    10441 ccgcggatca aggataattt tgcggcttac cgtactcgtc agtttcagtg caacaactgc
    10501 acgcccaccc gcagcccagg ctgccagtgg cgtgactgtc agtggctctg acggatagag
    10561 tgtggtgatt ttcccgggca gatgtgtatt aaccggccgg atacccggta ccgcttccag
    10621 cgtccgtaac ggtgcgtaca gcgactgtgc cgcatagcgg gtcaacagaa cgggtaaagg
    10681 ctctgcatac ttcaccggct gacggagggc ctgcgagccg tcatccccgc cgggtaccac
    10741 ctttttgtgt tctgcagagc gtgcagacgc cgctgtgcca gcctgactga ccgtactgac
    10801 gcttccctca tacacaatcc gtacgggctc tgtgggccct ttttctgctg ctgtcacatc
    10861 cagcagaata atttctccgc tctccacatc ctgcagttgc agacgcgtct gcggaaaaac
    10921 actctctgct ttcagataga ctgccccacc ggtactctgt accctcaact ttccgttcag
    10981 tgctggcggg aagcccaccc gcacattttt atctgcaaaa ataacccgct cctgtcctgc
    11041 catcagggta acgggtaagg gaatgcgctc ccatttcatc agctcatcag cccgtgcgct
    11101 cagtggtaaa accagcagtg acaggaccat cacaatacta cggtataaac aacgttcagc
    11161 tccgttcatc actgattatc ccccttcccg gctgccgggg aggctgcttc cagccgctgt
    11221 ggtgtactgc tgtagcagtc cagcgccagc ccgaacggat tacgttcagg atccccctcc
    11281 atgcgcacca cctttatcgg ataacggaca agtgcccgtt ttaccggctc agcatgaaaa
    11341 tactcatctg ccaccagatc cagctgtacc tgccagttat cccgcccttc caccgtcacc
    11401 ggatttttgt tgtacctgcc cccgtagcca tgttccggaa tttcatacac cacgcgcaca
    11461 cgctccagca gctcccccgc atcactgcga cggcgggcat cactctccag aatctcccga
    11521 caggatggcg tcaggtaggc agagagcgca tgaattttcg ccgggtagtc cacctctccg
    11581 ttcttcggcc aggcattcag ttgctgaaag atataaaagg caaaggaata aaccgttgac
    11641 ggcgggactt cccaccaggg acgggtactg ccggaacgca gatccggcgg attgtgaatt
    11701 gtcagtctgt ccggtgccgt catccacccg atacagacca gcatcagaat tccggccagc
    11761 acaccgctga aaatacgcag tgtcaggata tgctggtcgc gatcccgcag agcatgacga
    11821 aaacgactca ttttttcccc ctgctacggg tacggcgcag ggaccagcca tgcgcctccc
    11881 ggataaatgt tttccggcca ccaccccatg cactgctttt cttcgccaga tgctgccaga
    11941 gccagttgtc aggttttccc cgcttcagcc ggctgatcca gacgccaccg aaccagacca
    12001 gcagcagggg agtcagcaac atccctgtcg ggataatcac ccagccaagt agaggaatca
    12061 gcggtaacgg ccagatcccc cctgccacag cgcctgtcag ggccgccagc cccagttccg
    12121 gtgtggtaaa accacggaaa accgcaggct ccacgttcag acgatcgggc agaaaacgaa
    12181 tggtctgcat ggggatgtct ccttacagaa tgatggaggc cgacttgccc agcagccaga
    12241 taatggcgac cagcagaaca acaccaacca ccacgatggc accaaactgc ccccatccgg
    12301 ccttcccgtc acgaacctct gagaaggtgg aaagcgccgc ttccgccacc ttcacaaaag
    12361 caactgcact gagcacaaga ccaaacagca caacaccatc ctggagatag cctttgatct
    12421 gtccgaaaag tccaccacct ccgccggatg ttggcggctc tatctgaggc aaatcagcca
    12481 gagcaggtgt gatcccggta cacagaaaca ccgccatggc ataccaggcc tggcgacaga
    12541 tgcgcatttt tttatgcatt atttcctcct gaaacttaaa caagacactg aaatcaaccc
    12601 gcaaacatcc agatactgca gatcagcagc aggacgagtc ggaacataaa ccagccgaac
    12661 acaccatccc ggacatcacc gctacgccag cctgcccagg catccgtcag actccacgct
    12721 gcccagagga acacaactgc cagcagaaat cccagcgtga tgagatgcag cacactgaga
    12781 tccacttcac ctgaagcagc ccggaaggcc gtattctgca gggccgtcat cgccatcagg
    12841 gacgctcccg ccggtagtca ccggtcacgg ctgcggaaga ccggggttgc gcccgggaag
    12901 gggccagata atggtcaata ccgtcacgaa tggtctgcag atctgcacgg gcacgcagat
    12961 aatcaaaaaa gtagcgttcc ccctgtgaag aagcctgcag agaggccgtt ctggcccgct
    13021 ccagcgctgc ctgcacgtgg tcaagctggc gcattacagt ggccagttca tccttttcgg
    13081 cagcctgcgc aggtaatgca aaggacaaaa gaaacagcat ccccgcggca ccggccagac
    13141 ggccggcacg gcagggtgat gccggaaaag aaaacatacg cacctccgtc agagttaaac
    13201 agtgcgtaca ggatgcgtaa ggcagaggac tggatcagcg ataaactcta tttgcaaaac
    13261 agaaaatttt tacgggagaa tacactggtg tggatgaagg caaacaac
//
LOCUS       DAZGHF010000127        13306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGHF010000127 DAZGHF010000000
VERSION     DAZGHF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.128"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            380..715
                     /gene="yjdM"
                     /locus_tag="ACHK3A_003907"
     CDS             380..715
                     /gene="yjdM"
                     /locus_tag="ACHK3A_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO8741932.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            875..1318
                     /gene="yjdN"
                     /locus_tag="ACHK3A_003908"
     CDS             875..1318
                     /gene="yjdN"
                     /locus_tag="ACHK3A_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO8741933.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            1451..2239
                     /gene="phnC"
                     /locus_tag="ACHK3A_003909"
     CDS             1451..2239
                     /gene="phnC"
                     /locus_tag="ACHK3A_003909"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO8741934.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2264..3280
                     /gene="phnD"
                     /locus_tag="ACHK3A_003910"
     CDS             2264..3280
                     /gene="phnD"
                     /locus_tag="ACHK3A_003910"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8741935.1"
                     /translation="MNAKIIASLTFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKGNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3386..4165
                     /gene="phnE"
                     /locus_tag="ACHK3A_003911"
     CDS             3386..4165
                     /gene="phnE"
                     /locus_tag="ACHK3A_003911"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO8741936.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4186..4911
                     /gene="phnF"
                     /locus_tag="ACHK3A_003912"
     CDS             4186..4911
                     /gene="phnF"
                     /locus_tag="ACHK3A_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO8741937.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     HFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4912..5364
                     /gene="phnG"
                     /locus_tag="ACHK3A_003913"
     CDS             4912..5364
                     /gene="phnG"
                     /locus_tag="ACHK3A_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO8741938.1"
                     /translation="MHADTATRQHWMSVLAHSQPTELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLNADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5361..5945
                     /gene="phnH"
                     /locus_tag="ACHK3A_003914"
     CDS             5361..5945
                     /gene="phnH"
                     /locus_tag="ACHK3A_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO8741939.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5945..7009
                     /gene="phnI"
                     /locus_tag="ACHK3A_003915"
     CDS             5945..7009
                     /gene="phnI"
                     /locus_tag="ACHK3A_003915"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO8741940.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVQEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            7002..7847
                     /gene="phnJ"
                     /locus_tag="ACHK3A_003916"
     CDS             7002..7847
                     /gene="phnJ"
                     /locus_tag="ACHK3A_003916"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO8741941.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMNMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7844..8602
                     /gene="phnK"
                     /locus_tag="ACHK3A_003917"
     CDS             7844..8602
                     /gene="phnK"
                     /locus_tag="ACHK3A_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO8741942.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8713..9393
                     /gene="phnL"
                     /locus_tag="ACHK3A_003918"
     CDS             8713..9393
                     /gene="phnL"
                     /locus_tag="ACHK3A_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO8741943.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGVSS"
     gene            9390..10526
                     /gene="phnM"
                     /locus_tag="ACHK3A_003919"
     CDS             9390..10526
                     /gene="phnM"
                     /locus_tag="ACHK3A_003919"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO8741944.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGKIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTEAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10526..11083
                     /gene="phnN"
                     /locus_tag="ACHK3A_003920"
     CDS             10526..11083
                     /gene="phnN"
                     /locus_tag="ACHK3A_003920"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO8741945.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            11070..11504
                     /gene="phnO"
                     /locus_tag="ACHK3A_003921"
     CDS             11070..11504
                     /gene="phnO"
                     /locus_tag="ACHK3A_003921"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO8741946.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYQLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            11506..12264
                     /gene="phnP"
                     /locus_tag="ACHK3A_003922"
     CDS             11506..12264
                     /gene="phnP"
                     /locus_tag="ACHK3A_003922"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO8741947.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACVRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHSGLLDFSHTVEPFVMFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVA"
     gene            complement(12292..12621)
                     /gene="yjdP"
                     /locus_tag="ACHK3A_003923"
     CDS             complement(12292..12621)
                     /gene="yjdP"
                     /locus_tag="ACHK3A_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO8741948.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12690..13139)
                     /gene="rpiB"
                     /locus_tag="ACHK3A_003924"
                     /gene_synonym="alsI"
     CDS             complement(12690..13139)
                     /gene="rpiB"
                     /locus_tag="ACHK3A_003924"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO8741949.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASHVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         2992 a         3964 c         3680 g         2670 t
ORIGIN      
        1 attttattac ttttatttag ccagattgtt tttgagttct gttttcggct tttataatta
       61 ctgcaggaaa taattttata tttagcgtgt tgttttttat tagaacaaat aacatcttcc
      121 aatacgttta aaacgtcaga aagataaaaa catcatgtga attaaaaaaa gaacgagtag
      181 agcattaacc ttatcttaaa taataaatgg aggcaaaaag attattttct ttttgcgttt
      241 cctttcaaat gaaaacgatc gtcgtctaaa atcagcagta cccccgacaa actcagggat
      301 tttgtgtata attgcggcct ttttcggcaa tctgccgttt tttggcgctt ttgccctgct
      361 gacttttgag gaaatccaca tgtcattacc acactgccca aaatgcaact ccgaatacac
      421 ctacgaagat aacggcatgt acatctgccc ggaatgtgcc tacgaatgga acgacgcaga
      481 acctgcacag gaaagcgacg agctgatcgt taaagatgct aacggcaatc tgctggctga
      541 cggcgacagc gttaccatca ttaaagatct gaaagtgaaa ggtagctctt cgatgctgaa
      601 aattggcacc aaagtgaaaa acatccgcct ggttgaaggc gaccataaca tcgattgcaa
      661 aatcgacggt tttggtccga tgaaactgaa gtctgagttt gtgaaaaaga actgattgta
      721 ttgtgatcgg taggccggat aaggcgttta cgccgcatcc ggcaacggtg gcgactgcct
      781 gatgcgacgc tgaccgcgtc ttatcaggct tccctctcat ctgtataaat ttcgaactac
      841 acttaacggg cttctcttaa ctgaggtcac catcatgccg ttaagtccct acctctcttt
      901 tgccggtaac tgttccgacg cgattgccta ttatcaacgt acgttgggcg cggaactgct
      961 ctataaaatc agcttcggcg aaatgccgaa atcagcgcag gacagcgccg agaactgccc
     1021 ttccggaatg caatttcccg ataccgccat cgctcatgcc aacgtgcgca ttgcaggaag
     1081 cgacatcatg atgagcgatg ctataccgtc aggaaaagcc agctactccg gctttacgct
     1141 agtgctcgat tcgcaacagg tcgaagaagg aaaacgctgg tttgataacc ttgcagctaa
     1201 cggaaaaatc gaaatggcct ggcaggaaac tttctgggcg catggcttcg gcaaagtcac
     1261 cgataaattt ggcgtaccgt ggatgattaa tgtcgtcaaa caacaaccaa cgcactaacc
     1321 cgccgggagg gccgccctcc cgcactgtca tcgaaatctc gttaactctt catccgttag
     1381 tcacttttag ttaacaaaat cgtcacaata atccgccacg atggagccac ttttttaggg
     1441 aggctgcatc atgcaaacga ttatccgtgt cgagaagctc gccaaaacct tcaatcagca
     1501 tcaggcgctg cacgcggttg atctgaacat tcatcacggt gaaatggtgg ctctgcttgg
     1561 gccgtcgggt tccggcaaat ccaccctttt acgtcactta agcggtttga ttaccggcga
     1621 taaatctgcc ggtagccata tcgagctgct gggccgcaca gtccagcgcg aaggccgcct
     1681 ggcccgcgat atccgcaaaa gccgcgccaa taccggctac atcttccaac aattcaacct
     1741 ggtgaatcgc ctgagcgtac tggagaacgt gctgattggc gcgctcggca gcacgccatt
     1801 ctggcgcacc tgttttagct acttcacccg cgagcagaaa cagcgcgcgt tacaggcgct
     1861 gacccgcgtt ggcatggtgc actttgccca tcagcgcgtt tccaccctct ccggcggaca
     1921 gcaacagcgt gtggcgattg cccgcgcgct gatgcagcag gcgaaggtga ttctggcgga
     1981 cgaacccatc gcctcgctgg acccggaatc ggcgcgcatc gtgatggaca ccctgcgcga
     2041 cattaaccag aacgacggca ttaccgtggt cgtcacgctg catcaggtgg attacgccct
     2101 gcgctactgc gaacgcatcg tcgccctgcg ccaggggcac gtcttctacg acggcagcag
     2161 ccaacagttt gataacgaac gttttgacca tctctaccgc agcatcaacc gcgtcgaaga
     2221 aaacgcgaaa gctgcctgac atccccatca ttgaggaaaa cgaatgaacg ctaagataat
     2281 tgcctcgctg accttcacca gcatgttcag cctcagcacc ctgttaagcc cggcgcacgc
     2341 cgaagagcag gaaaaggcgc tgaatttcgg cattatttca acggaatcac agcaaaacct
     2401 gaaaccgcaa tggacgccgt tcttacagga tatggagaag aagctgggcg tgaaggtcaa
     2461 cgccttcttt gccccggact acgcgggcat tatccaggga atgcgcttca acaaagtgga
     2521 tatcgcctgg tacggcaatc tgtcagcgat ggaagcggtg gatcgcgcca atggccaggt
     2581 cttcgcccag acggtcgcgg cggatggatc gccgggttac tggagcgtgt tgatcgtcaa
     2641 caaagatagt ccgatcaaca acctgaacga tctgctggcc aagcgtaaag atctcacctt
     2701 cggcaatggc gatcctaact ccacctcagg cttcctcgtc cccggctact acgtcttcgc
     2761 caaaaacaat atctccgcca gcgacttcaa gcgcaccgtc aacgccgggc atgaaaccaa
     2821 cgcgctggcc gttgccaaca agcaggtgga tgttgccacc aacaacaccg aaaacctcga
     2881 caagctgaaa acctccgcac cggagaagct caaagaactg aaggtgatct ggaaatcgcc
     2941 gctgatccca ggcgatccga tcgtctggcg caaaaatctg tccgaaacca ccaaagacaa
     3001 gatctacgac ttctttatga actacggcaa aacgccggaa gagaaagcgg tgctggaacg
     3061 cctgggctgg gccccgttcc gcgcctccag cgacctgcaa ctggtgccga ttcgccagct
     3121 cgcgctgttt aaagagatgc agggcgtgaa aggcaataaa gggctgaatg agcaggacaa
     3181 gctggcaaaa accagcgcca ttcaggcgca actggatgac ctggaccgcc tgaacaacgc
     3241 gttaagcgcg atgagttcgg tgagtaaagc ggtgcagtaa atcgtaggtc ggataagacg
     3301 ccccggcgtc gcatccgaca atgtgcagac gttgatgccg gatgcggtgc aagcacctta
     3361 tccggcctac agaccggagc caaacatgca aaccatcacc atcgccccac ccaagcgcag
     3421 ctggttctcg cttctgagct gggccattgt tctcgccgtg ttggtcgtct cgtggcaggg
     3481 cgcggaaatg gccccgctta cgctgatcaa agacggcggc aacatggcaa ccttcgccgc
     3541 cgacttcttc ccgcccgatt tcagccagtg gcaggattac ctcaccgaaa tggccgtcac
     3601 gctgcaaatc gccgtctggg gcaccgcgct ggcggtggtt ctctccatcc ccttcggtct
     3661 gatgagcgcc gaaaacctgg tgccgtggtg ggtttaccag cccgtacgcc gcctgatgga
     3721 cgcctgccgc gccattaacg aaatggtctt cgccatgctg ttcgtggtcg ccgtcggcct
     3781 tggcccgttc gctggcgtgc tggcgctgtt tatccacacc accggcgtgc tctccaagct
     3841 gctttccgaa gcggtagaag cgatcgaacc cggcccggtg gagggcattc gcgccaccgg
     3901 tgccaacaag ctggaagaga tcctctacgg cgtgctgcca caggtgatgc cgctgctgat
     3961 ctcctactcc ctctatcgct tcgaatccaa cgtccgctcg gcgaccgtcg tcggcatggt
     4021 cggtgcaggc gggatcggcg tcaccctgtg ggaagcgatt cgcggtttcc agttccagca
     4081 aacctgcgcc ctgatggtgc tcatcatcgt cacggtcagc ctgctggatt tcctctctca
     4141 acggttgcgt aagcacttta tctgataagc gaggcattga tagctatgca cttgtctaca
     4201 catccgacca gctacccaac acgctatcag gagatagccg caaaacttga gcaggagctt
     4261 cgtcaacact accgctgcgg cgactatctt cccgccgagc agcaactggc agcgcgcttt
     4321 gaggtgaacc gccacaccct gcgccgcgcc atcgaccaac tggtggaaaa aggttgggta
     4381 cagcgccgtc agggcgtcgg cgtgctggtg ctgatgcgcc cgttcgatta cccgctcaac
     4441 gcccaggcgc gttttagcca gaacctgctg gaccaaggca gccatcccac cagcgaaaaa
     4501 ttgctatcgg tgctgcgccc cgcctccggt cacgtcgctg acgcgctggg gataaccgag
     4561 ggggagaacg tcatccacct gcgcaccctg cgccgggtca acggcgtcgc gctctgttta
     4621 atcgaccact actttgcgga cctcaccctc tggccgacgc tgcaacattt cgacagcggt
     4681 tcgctgcacg attttctgcg cgagcaaacg gggattacgc tacgccgcag tcagacgcgg
     4741 atcagcgccc gccgcgccca ggccaaagag tgccagcgtc ttgaaatccc gaatctatcg
     4801 ccgctgctgt gcgtgcgcac ccttaaccac cgtgacggtg aaagcagccc ggcggagtac
     4861 tccgtcagcc tgacgcgcgc cgacatgatt gaattcacta tggagcactg aatgcacgca
     4921 gataccgcga cccgccagca ctggatgtcc gtactggcgc acagccaacc gactgagctg
     4981 gcagcccgcc tgaacgcgct gaacatcacc gccgactatg aagtaatccg cgccgctgaa
     5041 accggcctgg tgcagattca ggcgcggatg ggtggtaccg gcgaacgctt ttttgctggc
     5101 gacgccacgc tcacccgcgc cgccgtacgc ctgactgacg gcacgctcgg ctacagctgg
     5161 gtgctggggc gcgacaaaca gcacgccgaa cgctgcgcgc tgattgacgc gctgatgcag
     5221 caaacccgcc attttcaaaa cttatcagaa actcttattg ccccgctgaa cgctgaccgt
     5281 atggcacgca ttgccgcacg ccaggccgaa gtaaacgcca gccgggtcga cttctttacg
     5341 atggttcgcg gagataacgc atgaccctgg aaaccgcttt tatgcttccc gtgcaggatg
     5401 cccagcacag ttttcgtcgc ctgttaaaag ccatgagcga gccgggcgtg attgtcgccc
     5461 tgcatcagct caaacgcggc tggcagccgc tgaatatcgc caccaccagc gtgctgctga
     5521 cgctggccga taacgacacg ccggtgtggc tttctgcgcc attaagtaac gatatcgtca
     5581 gccagagcct gcgttttcat accaacgcgc cgctggtcaa tcagccggaa ctggcgacct
     5641 tcgcggtaac ggatgaggcg atttccagcg aacagcttaa cgccctttcc agcggcaccg
     5701 ctgttgcgcc agaagcgggc gcgacgctga ttttacaggt cgtcagcctg agcggcgggc
     5761 gcatgctgcg ccttactggc gcgggtattg ccgaagaacg aatgatcgcc ccgcagctgc
     5821 cggagtgcat tctgcacgaa cttaccgagc gcccgcaccc attcccgctc ggcatcgacc
     5881 tgatcctgac ctgcggcgag cgcctgctgg ctatcccgcg aaccactcat gtggaggtat
     5941 gctgatgtac gttgccgtga aagggggcga gaaggcgatc gacgccgccc acgccctgca
     6001 agagagccga cgccggggca ataccgattt gcctgaactg agcgtcgccc agattgaaca
     6061 gcagcttaat cttgcagtag atcgcgtgat gaccgaaggc ggcattgccg accgcgaact
     6121 ggcggcgctg gcgctgaaac aggccagcgg cgataacgtc gaagcgattt tcctgctacg
     6181 cgcctaccgc accacgctgg cgaagctcgc agtaagcgag ccgctcgaca ccacggagat
     6241 gcgtctcgaa cggcgtattt cagcggttta taaagatatt cccggcggcc agctgcttgg
     6301 cccaacctac gactacaccc atcgcctgct cgatttcacc ctgctggcaa acggcgaagc
     6361 gccgacgctg accacagccg acagcgaaca acagccgtcg ccgcacgttt tcagcctgct
     6421 ggcgcgtcag gggctggcga agtttgaaga agatagcggc gcacagccgg acgacatcac
     6481 ccgcacgccg ccggtttacc cctgctcacg ctcctcccgc ctgcaacaat taatgcgcgg
     6541 cgacgaaggc tatttgctgg cgctggccta ctccacccag cgtggttacg gacgcaatca
     6601 tccgttcgca ggcgaaatcc gcagcggcta tatcgacgtg tcgattgtgc aggaagagct
     6661 gggatttgcg gtgaacgtcg gcgaactgct gatgaccgaa tgtgaaatgg tcaacggttt
     6721 tatcgacccg ccggatgagc cgccgcactt cacgcgcggc tacgggctgg tgttcggcat
     6781 gagcgagcgc aaagcgatgg cgatggcgct ggttgaccgc gctctacaag ccccggagta
     6841 cggcgagcac gcgacaggcc cggcgcagga tgaagagttc gtgctggcgc atgccgacaa
     6901 cgtcgaagcc gcaggctttg tctcgcacct taaactcccg cactacgtcg atttccaggc
     6961 cgaactggag ctactcaaac gtctgcaaca ggagcagaac catggctaat ctgagcggct
     7021 acaactttgc ctacctcgac gagcagacca aacgcatgat ccgccgcgcc atcttaaaag
     7081 cggtggcgat ccccggttat caggtgccgt ttggcgggcg cgagatgccg atgccgtacg
     7141 gctggggaac cggcggcata cagcttaccg ccagcgtgat tggcgaaagc gatgtgctga
     7201 aggtgattga ccagggcgcg gacgacacca ccaacgcggt gtcgatccgt aacttcttta
     7261 agcgcgtgac cggggtaaac accactgaac gtacggacga tgcgacgctt atccagacgc
     7321 gccaccgcat ccccgaaacg ccgctgaccg aagatcagat cattattttc caggtgccga
     7381 tccccgaacc gctgcgcttt atcgagccgc gcgaaacgga aacccgcacc atgcacgcgc
     7441 tggaggagta cggcgtgatg caggtgaagc tgtatgaaga tatcgcccgc ttcggtcata
     7501 tcgccaccac ctacgcctat ccggtgaagg taaacgggcg ctacgtaatg gacccgtcgc
     7561 cgatcccgaa atttgataac ccgaaaatga acatgatgcc cgccctgcaa ctgttcggcg
     7621 cggggcgcga gaagcgcatc tatgcggtgc cgccgtttac ccgcgtggaa agcctcgatt
     7681 tcgacgatca cccgttcacc gttcagcagt ggaatgagcc atgcgccatc tgcggatcga
     7741 cccacagcta tcttgatgaa gtggtgctgg atgacgccgg aaaccgcatg tttgtctgct
     7801 ccgataccga ttattgccgc caacagagcg aggcgaaaag ccaatgaatc aaccgttact
     7861 ttcggtcaat aacctgaccc acctttacgc gccgggcaaa ggctttagcg atgtctcttt
     7921 tgatttatgg ccgggggaag tgctgggcat cgtcggggaa tccggctccg ggaagaccac
     7981 gctgctgaag tcgatctccg cgcgcctgac gccgcagcaa ggggaaattc gctatgaaaa
     8041 ccgttccctc tatggcatga gcgaggccga ccgccgtcgc ctgctgcgca ccgaatgggg
     8101 cgtggtgcat cagcatccac tcgacggcct gcgccgccag gtgtcggcag gcggcaatat
     8161 cggcgagcgg ctgatggcga ccggggcgcg tcattacggc gatattcgtg ccaccgcgca
     8221 gaagtggctg gaagaggtgg aaattcccgc caaccggatc gacgacctgc cgaccacctt
     8281 ttccggcggt atgcagcagc gtttgcagat tgcccgcaac ctggtgacgc atccgaagct
     8341 ggtatttatg gacgagccaa ccggcgggct ggatgtatct gtgcaggcgc gcctgctcga
     8401 cctgctgcgc ggcctggtgg tggagctgaa cctggcggtg gtgattgtca cccacgattt
     8461 aggcgtcgcc cgcctgctgg cggaccgttt gctggtgatg aagcaggggc aagtggtgga
     8521 gagtgggtta accgaccgcg tgctcgacga cccgcaccat ccgtatacac aactgctggt
     8581 gtcatcggtt ttgcagaatt gagccggtgc cggatgcggc gtaaacgcct tatccggcct
     8641 acaaatgcgc tccccgtagg tcggataaga cgcgtcagcg tcgcatccga cacccgaacc
     8701 acgaggcgaa aaatgattaa cgtacaaaac gtcagtaaaa ccttcatcct gcatcagcaa
     8761 aacggcgtgc gcctgcccgt cttacaacgt gcctcgctca ccgtcaacgc aggcgaatgc
     8821 gtggtgcttc acggccactc cggcagcgga aaatcaaccc tgctgcgctc gctgtacgcc
     8881 aactatctgc ccgacgaagg gcaaatccag atcaaacacg gtgaggaatg ggtagacctg
     8941 gtcaccgcgc cagcccgcaa agtggtggag atccgcaaaa ccaccatcgg ctgggtgagc
     9001 cagtttctgc gcgtcatccc gcgtatttcg gcactggaag tggtgatgca gcctctgctc
     9061 gataccggcg taccgcgtga agcctgcgcc gctaaagccg cccgattact gacgcgcctg
     9121 aacgtgcctg aacgcctgtg gcacctggca ccatcgacct tctccggcgg cgaacagcag
     9181 cgcgtcaaca tcgcccgtgg ctttatcgtc gactatccga ttctgctgct ggatgaaccc
     9241 accgcctcgc tggacgctaa aaacagcgcc gcggtggtgg aattaattcg cgaagccaaa
     9301 gcccgtggcg cggccatcgt cggtatcttc catgacgaag ccgtacgcaa tgacgtcgcc
     9361 gaccgcctgc acccaatggg agtctcttca tgattatcaa taacgtcaaa ctggtgctgg
     9421 aaaacgaagt ggtgcacggt tcactggaag tacaggatgg caaaattcgc gcctttgccg
     9481 aaagccagag ccgccagccg gaagcgatgg acggcgaagg tggctggctg ctgccggggc
     9541 tgattgagct gcataccgat aatctggata aattcttcac cccgcgtcca aaagtggact
     9601 ggcccgccca ctcggcaatg agtagccacg acgcgctgat ggtggcgagc ggcatcacca
     9661 ccgtgctgga cgcggtggcg attggcgacg tgcgcgacgg cggcgatcgg ctggagaatc
     9721 tggagaagat gatcaacgcc atcgaagaga cgcagaaacg cggcgtcaac cgcgccgagc
     9781 accgtctgca tctgcgctgc gaactgccgc atcacaccac gctgccgctg tttgaaaaac
     9841 tggtgcagcg cgaaccggtg acgctggtgt cgctgatgga tcactcgccg ggccagcgcc
     9901 agttcgctaa ccgcgagaag tatcgcgaat attatcaggg caaatactcg ctcaccgaag
     9961 cgcagatgca gcagtatgaa gaagagcagc tggcgctcgc cgcacgctgg tcgcagccaa
    10021 atcgcgaaac catcgccgca atgtgccgcg cgcgacacat tgcgctcgcc agccacgatg
    10081 acgccacgca cgcccacgtt gctgaatctc accaacttgg cagcgtaatc gccgaatttc
    10141 ccaccacgtt cgaagcggca gaagcctcgc gcaagcatgg catgaacgtg ctgatgggcg
    10201 caccgaatat cgtgcgcggc ggttcgcact ccggcaacgt ggcggccagt gaactggcgc
    10261 agcttggcct gctggatatc ctctcttccg actactaccc cgccagcctg ctcgatgcgg
    10321 catttcgcgt cgccgatgac gagagcaacc gctttacgct gccgcaggcg gtgaagctgg
    10381 tgactaaaaa tccggcgcag gcgcttaatc ttcaggatcg cggggtgatt ggcgagggca
    10441 aacgcgccga cctggtgctg gcgcatcgca agggcaatca cattcatatc gaccacgtct
    10501 ggcgtcaggg taaaagggtg ttctgatgac gggaaaactg atttggttaa tgggggcctc
    10561 tggctccgga aaagacagtc tgttgacgga actccgccag cgggaacaaa ctcagctact
    10621 ggtagcgcat cgctacatca cgcgcgccgc cagcgccgga agtgaaaacc atatcgccct
    10681 gagcgagcag gagtttttta cccgcgcggg gcagaacctt ctggccttaa gctggcacgc
    10741 taacggtctg tattatggcg tcggcgtcga gattgacctc tggctgcacg ctggatttga
    10801 cgtggtggtc aacggctcac gcgcccatct gccgcaggcg cgggcgcgct atcaatcggc
    10861 gctgctgccc gtttgtttac aggtttcgcc agagatcctg cgccagcgcc tggaaaaccg
    10921 tggtcgtgaa aatgccagtg aaattaacgc ccggctggcg cgcgctgccc gctatactcc
    10981 tcaggattgt cttacgctca ataatgacgg cagcctgcgc cagtcggtcg acaagttgct
    11041 gacgctgatt catcagaagg agaaacacca tgcctgcttg tgagcttcgc ccggctacgc
    11101 agtacgacac cgacgcagtt tacgcgctga tttgtgagct aaaacaggcg gagtttgacc
    11161 accaggcgtt tcgcgtgggg tttaacgcca atctgcgcga tccgaacatg cgctaccaac
    11221 tggcgctgct ggatggcgaa gttgtcggca tgatcggcct gcatttgcag tttcatctgc
    11281 atcatgtcaa ctggatcggc gaaattcagg aactggtggt gatgccgcag gcgcgcggtc
    11341 tgaatgtcgg cagcaaatta ctggcatggg cagaagaaga agcccgccag gccggggccg
    11401 aaatgactga gctttccacc aacgtgaagc gccacgacgc gcaccgattt tacctgcgcg
    11461 aaggctacga gcagagccac ttccgcttca ccaaggtgct gtaacatgag cctgaccctc
    11521 acacttactg gcactggcgg cgcacagggc gttcccgcat ggggctgtga gtgcgtggcc
    11581 tgcgtcagag cgcggcgctc accgcagtat cgccgccagc catgcagcgg cgtcgtgaag
    11641 tttaacgacg cgatcaccct gatcgacgcc gggcggcacg atctcgccga tcgctggtcg
    11701 cccggatcgt tccagcagtt tttgctgacg cactaccata tggatcacgt gcaggggctg
    11761 tttccgctgc gctggggcgt gggcgatcag atcccggtct acggcccgcc ggatgaacag
    11821 ggctgcgacg atctattcaa acattcgggc ctgcttgatt tcagccacac ggtggagcca
    11881 tttgtgatgt ttgatttgca gggattacag gtcacgccgc taccgctcaa ccactcaaaa
    11941 ctcactttcg gctatttact ggaaacggcg cacagccggg tggcgtggct atctgacacc
    12001 gcaggcctgc cggaaaaaac gctgaaattt ttacgcaata atcagccgca ggtaatggtg
    12061 attgattgca gccacccgcc gcgcgaggat gcaccgcgta atcactgtga tttaaatacc
    12121 gtgcttgcgc tgaatcaggt tatccgctcg ccgcgggtga ttctgaccca tatcagccat
    12181 cagtttgatg cgtggctgat ggaaaacgcg ctaccatcgg ggtttgaggc ggggtttgat
    12241 gggatggaga ttgaggtggc gtgagggaaa atacccgcgc cggcccctat atcaacgccc
    12301 atactcatct tccattctcc gctcttcatc ttccagttgc cgacgctcct gatcaagctg
    12361 gcgctggcga tcgtccagct gcctgcggcg atcttcaaac tggcggcggc ggtcatcata
    12421 ttgtctgcgc cgatcgtcgc tcacttcacg ctgccagccg tcgtcgcgcg aatcttcata
    12481 gtctcgccca cggtcagggt tataagcgtc gttaatcgcc tgctgaatat tgccaatggt
    12541 gtcgtcgata atatcggcct gggccggaac ggtggacagc gtgagcaggg tgaataaaag
    12601 aaatagcgga aagcgtttca ttagccaacc tcaaaaagaa actctatcca cattagtcat
    12661 tactcatcca tgcaagtagt ggacgaatct caatttctcc gctgctctat tgccgtaatc
    12721 gcctccacgc gttgttgatg acgaccgcct tcgtactgtg cgcccagcca cgcatcgaca
    12781 atcatttttg ccagttcgag gccaaccact cgtgaaccaa aagccagcac gttggtgtcg
    12841 ttatgctgcc gcgaaagttg cgcggaataa ggttcgctac aaacgaccgc gcgaattccg
    12901 gcaaacttgt tcgccgctat cgaaataccg acgccagtgc cacaaatcaa aatcccgcca
    12961 tcagcctctc cgccagcaac ggccagtgcg acgtgactgg cgtaatgcgg ataatcagta
    13021 cgctctgacg accaggttcc tttatcaatc acttcaacgc cacgctcaac taaatgtgcc
    13081 actatttcat gttttaaaat gaaaccgaca tgatcacagc caaatgcaat ctttttcatt
    13141 catcttcccc gcccgcgcaa aacttcacaa tcaaaaaaca tttgtgatgt aaatgaatta
    13201 aaaacaccca aaatcgatga aatccgcctt cccgatctgt tttacagagt tacatctggc
    13261 agcgcaatag cgttaaagac actcactatc cagcaacatc tatcat
//
LOCUS       DAZGHF010000128        13245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGHF010000128 DAZGHF010000000
VERSION     DAZGHF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.129"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1838)
                     /gene="ccmF"
                     /locus_tag="ACHK3A_003925"
     CDS             complement(<1..1838)
                     /gene="ccmF"
                     /locus_tag="ACHK3A_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO8741950.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAMLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRNGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRW"
     gene            complement(1835..2314)
                     /gene="ccmE"
                     /locus_tag="ACHK3A_003926"
     CDS             complement(1835..2314)
                     /gene="ccmE"
                     /locus_tag="ACHK3A_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO8741951.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(2311..2520)
                     /gene="ccmD"
                     /locus_tag="ACHK3A_003927"
     CDS             complement(2311..2520)
                     /gene="ccmD"
                     /locus_tag="ACHK3A_003927"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO8741952.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVILVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(2517..3254)
                     /gene="ccmC"
                     /locus_tag="ACHK3A_003928"
     CDS             complement(2517..3254)
                     /gene="ccmC"
                     /locus_tag="ACHK3A_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO8741953.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(3296..3958)
                     /gene="ccmB"
                     /locus_tag="ACHK3A_003929"
     CDS             complement(3296..3958)
                     /gene="ccmB"
                     /locus_tag="ACHK3A_003929"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO8741954.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(3955..4578)
                     /gene="ccmA"
                     /locus_tag="ACHK3A_003930"
     CDS             complement(3955..4578)
                     /gene="ccmA"
                     /locus_tag="ACHK3A_003930"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO8741955.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAENKIRRISLTQTRGE"
     gene            complement(4591..5193)
                     /gene="napC"
                     /locus_tag="ACHK3A_003931"
     CDS             complement(4591..5193)
                     /gene="napC"
                     /locus_tag="ACHK3A_003931"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO8741956.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(5203..5652)
                     /gene="napB"
                     /locus_tag="ACHK3A_003932"
     CDS             complement(5203..5652)
                     /gene="napB"
                     /locus_tag="ACHK3A_003932"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO8741957.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(5649..6512)
                     /gene="napH"
                     /locus_tag="ACHK3A_003933"
     CDS             complement(5649..6512)
                     /gene="napH"
                     /locus_tag="ACHK3A_003933"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO8741958.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPASVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(6499..7194)
                     /gene="napG"
                     /locus_tag="ACHK3A_003934"
     CDS             complement(6499..7194)
                     /gene="napG"
                     /locus_tag="ACHK3A_003934"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO8741959.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(7201..9687)
                     /gene="napA"
                     /locus_tag="ACHK3A_003935"
     CDS             complement(7201..9687)
                     /gene="napA"
                     /locus_tag="ACHK3A_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO8741960.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPLSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(9684..9947)
                     /gene="napD"
                     /locus_tag="ACHK3A_003936"
     CDS             complement(9684..9947)
                     /gene="napD"
                     /locus_tag="ACHK3A_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO8741961.1"
                     /translation="MYTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(9937..10431)
                     /gene="napF"
                     /locus_tag="ACHK3A_003937"
     CDS             complement(9937..10431)
                     /gene="napF"
                     /locus_tag="ACHK3A_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO8741962.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLLFTIGEAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNACGACAASCPVSAITAEY
                     LHVH"
     gene            10540..10704
                     /locus_tag="ACHK3A_003938"
     CDS             10540..10704
                     /locus_tag="ACHK3A_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741963.1"
                     /translation="MHTPIGVKPVAGSKEWQEAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCFLIRI"
     gene            10818..11327
                     /gene="eco"
                     /locus_tag="ACHK3A_003939"
     CDS             10818..11327
                     /gene="eco"
                     /locus_tag="ACHK3A_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO8741964.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(11477..13123)
                     /gene="mqo"
                     /locus_tag="ACHK3A_003940"
     CDS             complement(11477..13123)
                     /gene="mqo"
                     /locus_tag="ACHK3A_003940"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO8741965.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT         3184 a         3893 c         3349 g         2819 t
ORIGIN      
        1 atccagcgaa caaacggttt gtagtaaaga cgcacggccc acgcgccgtt ttccagctct
       61 tcaccgaggg ccgcgtacag gtcacgcgtg atgccgccgt caattgccgc ttcggtcatc
      121 atcgacccgg cagtgttgta ataacgtttt tccgcataca gcactgtttc cggcttgcca
      181 tttcgcgtta cgccgatagt cgccacaccg ccacgccagt tcggaccagt cacctctttg
      241 acatcacgga aggtgaagcg atattcatga atatcgacgc tatcgccgga cttcatgcgc
      301 acatcacgct caacgctata gttctggcta aaggcaatac caacaattgt cactgccagc
      361 cccaggtgag ccgccaccat tccccaataa ctgaaggtgg ttttcgtgcc acgtgaaata
      421 cgcagcgcag cttccgcaat tgccagcatc gcaatccagc aggccattgc caggccgagc
      481 accgtcatcg ccacaacttt gctttcgaac agccacggca gcagcagcga cagcaccagc
      541 gttgagatga aggcgataat caataaattg cggattttac gtgggcgatc gcgcccccag
      601 cgcaccagag gaccgacacc aagcagcagc gcaaacggca ccatcagcca ggtaaacatg
      661 gtgttgaaga acggttcacc aatcgaaata ctgcccagtc ccagttgctt gtgcaccagc
      721 ggcagcagcg tccccagtaa taccaccagc atcgcggcga ccagcaaaac gttgttcgct
      781 aacagcagag attcccgcga ccacagcgca ttgtttacgc gtgagcgaac tttgtgtcca
      841 cgcgcggcaa acagcagcag cgaaccgcca atcaccaaca ccataaaggc gaggataaac
      901 ataccgcgcg ccggatcaga cgcaaacgcg tgtaccgata ccagcacgcc ggaacgcacg
      961 aggaaagtcc ccaacagaca caacgagaag gcactgattg ccagcagtaa tgtccacgct
     1021 ttgaagctgg cgcgttgttc agtgaccgcc agtgagtgca tcagcgcagt ccccaccagc
     1081 cacggcataa acgaggcgtt ttctaccgga tcccagaacc accagccgcc ccagccgagt
     1141 tcgtaatagg cccatgcgga accgagcaca atgccgagcg tcaagaagat ccacgccgcc
     1201 agcgtccacg ggcgggtaaa acgcgcatag gtgctgtcca gacgcccact caacaaagaa
     1261 gcaatggcga aagcaaacgc caccgagaaa ccgacgtacc ccatatagag cagaggcgga
     1321 tggaagatca gccccggatc ctgcaacagc gggttaagat cgcgcccttc aatcgggaaa
     1381 ttcggcagcg tgcgtgagaa cgggttagag gtaaagagaa tgaacagcaa aaagccgaca
     1441 ctgaccatcc ccattatcgc cagtacgcgg gccacaatat ccagcggaat acgctgacta
     1501 aaaatcgcta ccgcaaaggt ccagccgctc atcagtagca cccacagcag gagcgagcct
     1561 tcatgcgcgc cccaggtagc cgccacgcga taccacaccg gaagctgggt attggagttg
     1621 ctggcaacat aggtgacggt aaagtcgttg accacgaaag cattgaccag caccagaaat
     1681 gcgccagcca cagacataaa cagcagccag gcaaacaagc gggaagacgc catcatgcgc
     1741 gcgtccccgc gcgccacgcc ccatagcgga tacacagaca gcagcagcgc aattcctagc
     1801 gccagacaca gcagcccgtt accaatttct ggcatcatga tgctgggtcc ttataaacac
     1861 tcgccgggcg acggtggtta gcttccatcg ctttctcaac ttctggcggc gtgtagtttt
     1921 catcgtgttt cgccagcact tctttcgcga ggatatgatt gcctttttcc agctcgccct
     1981 gcaccacaac gccctgccct tcacggaaca gatccggcaa aatgccttcg taagagacat
     2041 ctactgaacc ttcagcgtcg taaatggtga aagttacttt tagcgaattg ggatcgcgct
     2101 gcacactccc cggcatcacc atcccgccaa cgcgcagacg ctgaccgact tccggcatct
     2161 gctgagtttc acgcttgccg tagagaatct cccccggcgt ataaaagaga tcgatattcg
     2221 agcgcagcgc atatagcacc agaccgatag tcagcgccag ccccgccagc acggcacagg
     2281 caatccacaa gcggttttta cggcgaatat tcatgcagcc tcctgctgtt gcgcagcacg
     2341 taaacgcgcc tcacgcgccc gctgttgcgc cacgccacgc agaattgcgc gatgctgcat
     2401 caccgagtgc acgaccaaaa tcaccagcgg aataacggtc atcaccaccg ccagccagac
     2461 aaaaaaggcg taaccgccca ttgcgaaaaa ttcattccag gaagcaaatg caggggtcat
     2521 ttacggcctc ttttcagtat cagttcactc acccacggac ggcgtttttc catcagcaaa
     2581 atcaaattac gcatccgcat cagcgtcagc gtggcagaca gcagcaggaa gccaaaaatc
     2641 gaccagcgca gcggcgaacg catcgccgga tcgatacttt gttgcatccg cgttgatccc
     2701 tgatgcaggg tgttccacca ctccacggag taatgaataa tcggcagatt caccacgcca
     2761 atcagcacca gaatacctgc cgcacggccc gccagacggc ggtcgtcgaa ggcgtgccac
     2821 agggcaatca cacccacata caaaaacagc agtaccagtt cagaagtcag gcgtgcatcc
     2881 catacccacc aggtgcccca catcggtttt ccccatgcag agccggtaac cagggcaata
     2941 aaggtaaaca cggcaccaat gggggccatc gccgccaccg ccaggttggc cattttcatc
     3001 tgccagacaa ggccaataaa cgccgccacc gccattgatg cataaatgcc catcgaccag
     3061 atcgccgcag gcacatgcag gtagataatg cggtagctat ttccctgctg ataatcagcc
     3121 ggagcaaaac cgaatcccca gatccagccg acggtaagca cgaccacact ggcaattgcc
     3181 agccacggta taaaccagcc acagatttga tacagccgtg gtgggatcgc cagttgatgc
     3241 agtgttttcc acatagtttc gataccagac tcgaacaaaa atcagtaatc cagcgttatt
     3301 gaatgctgat tcgtaacgct gccgccgtcg caaaaggact taatgtcgcg gtgcctgcca
     3361 gcaacgcgcc taaaattgcc agatacccat caacgggcaa atgcatagaa gccgcgtcca
     3421 tcgcggcggt ggcaaagatg agtaatggga tagtcagcgg taacaccagt atgctgagca
     3481 gcacaccacc gcgcttaagt cccactgtca gcgccacgcc cggcgcacca agaaagccaa
     3541 gcgtaggggt tcccagcagt agcgttaacg ccatcacttg ccagccataa acatccattc
     3601 ccagtagcat tgctaccagg ggcgaaagga tgagtaacgg cagaccggtt accatccagt
     3661 gcgccatcac cttcgccagc acaacggcgg gcaagggtaa cggcaacaac atcaattgtt
     3721 caagactgcc gtcctgcaaa tcgtcacgga acagtcgctc cagtgccagc aaggatgaaa
     3781 gcagcgcagc aacccagata atgcccggcg caatacgcgc cagcagttgc ggctccggac
     3841 cgatactgag cggaaaaagg gtaatgacaa tcaggaagaa ccacagcggg ttggcgattt
     3901 cggcgctatg gcgaaacgct acgcgcagct caagacggaa aatgcgccag aacattattc
     3961 gcccctcgtt tgcgtcagtg aaatgcggcg aattttattt tcagcaacgt tgagcggctg
     4021 gtgggtagtc agaatcacaa tccccccctg ctccgtatgc tgcgccatac gctgggtcag
     4081 acgatcgacg ccgttaacgt caatcgcggt aaaaggctcg tcgaggatcc ataacgtggc
     4141 acgggtcagc cacagacgcg ctaaagcgac gcggcgttgt tgcccggcag aaagttgatt
     4201 taccggaata tcttcgaatc cggcaagccc ggcctgcgcc agggcttcca gacattgtgc
     4261 ggtatcgcca tcgcgatgat aaaagtgcag attttctaac gccgtcagcc gggttttgat
     4321 ccccggctga tgccctatcc ataacaggtt ttgatggtag ctgtcgcgta cctgatgcaa
     4381 gggctgccct tgccagagaa cgtcacccgc atcagggcga gacaaccccg tcagcaaacg
     4441 gagaagcgtt gtcttccccg cgccgttgct accggtgatt tgtacccact ctcctgcgtt
     4501 cagcgtaaat gacaagccac taaataaggt tcgttcatcc cgctcgcaaa gtaactctct
     4561 ggcttcaagc atacccacgc aataaccctg ttaaaaacct ggctcgactt cgcgcatatc
     4621 gggcagcttg tgcgctatcc ctttatggca atcaatacag gtttgcccat ctttcaccgc
     4681 ctggtcatgc atcttcgcgg caaccgattt ctgggcggtt gtatccatat actcgaagtt
     4741 gtgacagtta cggcactcct gcgagttatt gtccttcatg cgccgccact cattctgtgc
     4801 catcgtcagg cgatgagctt caaatttctg cggcgtgtca ataacgccaa aaattttacc
     4861 atacagctct ttacttgctt tgagcttgcg tatcatcttc ggcacaaact cgtgcggaac
     4921 gtgacaatcc ggacaggtcg cacgaacgcc gctacggttg ttgtagtgca cggaatccat
     4981 gtattcctga tacaccgtgt tacgcatttc gtggcagcta atgcagaact cttcggtatt
     5041 ggctttttcc atcccggtgt taaagccgcc ccagaagacg atgccgccaa caaaaccgat
     5101 caacagcagc gtccccagcg ccagacggct gggggtacgc caccatttcc acaggcgctt
     5161 aatcagacca ggcttacggt cagaatttcc cataataacc tcttatttcc cgtaaccttt
     5221 tgatggggta aaggtattcc ccacgattgg cgcggtatcg gcctgcggta cgtgacattg
     5281 cagacagaaa taacgacgcg gagccacttc tgcgcccact ttgccgtcgc tgtccataaa
     5341 gtgagtagga ctgatacgcg gcgcgccagt ggtgcgatag ctttcgacac cgtggcactg
     5401 caagcagcgg ttggtattgg tcgttacctg ataaccttca acgctatgcg ggatcattgg
     5461 cggctgattc acatagttca gcggcatccg atcctgctct tttggcatcc gaatggcccc
     5521 ttcctgcgtc ccggatactt ccggcgactg gctaaaatcg acgccattag ccgcccaaac
     5581 cgcgccgctc accaccaggg ccagcatcgc cgtccattga cacagcgctt tcttcaggtc
     5641 atggcttttc atgatttcgc tcccgaactc catcgtgtag ttattgtaaa tacatcctca
     5701 gaacagacat ccacgcagcg accgcacgtc atgcaatcgc ggctggtgac ctgcaccggg
     5761 ctttgttcat ccagcaccgg ggcacgtagc acatgcggtt ccgggcaaac atgaaaacaa
     5821 tccatacagc ggttacattt ctgccgatcg gtggccgcaa cggtaatcac acctttgcta
     5881 cccagcacgc catacagcgc gccaaccgga caaatgtgcc cgcaccagcc gtgttcaacg
     5941 accagtagat caaataaaaa cagcgcgaga ataagcagcg cgccgctgcc gaatcccatt
     6001 accagactgc gccccatcag ggaaacgggg ttgatccatt cccagatgag cgtgccggtt
     6061 aaggccgatc ccagcagaat gaccaccaac agcacgtagc gaatgtggcg tgggatcgtc
     6121 gcagactgat tgaggtcaaa gcgcctgcgt aaccagttcg ctaagtcggt aatcgggttc
     6181 agcgggcaga cccagctgca aaataatcgc tttcctgcca gggcgtagag cacggtgata
     6241 atcaccgctc cggtcagcgc caccgaggcc ggcagatgac cgctggcaag actttgcagc
     6301 gtcatcagcg gatcggtcag cggaacggtg tcgaataaca ggctactgct gtagttgccg
     6361 tgcaagatcc acacaccaaa ccacggaccg ctcaaaaaca tccccagcac gaagaactga
     6421 caaaggcgac gtaacactaa ccaacggtga ctgcgccacc agcctttttt ctccagcgcc
     6481 tcgcgcccgg cgtcacgttt acgatttgcc attgttcccc tccagccagc cgaagcggta
     6541 atggtgacct aactcccctt tcgctagtga cagcggtaac accttgattg ccggttgttc
     6601 cagcacgcac actttttcgc acttaccgca accggtacag gcgtcgctgt gaaccgtcgg
     6661 cagaaagcgg gcgtgcttac cggtacgcgt gttgcgctcc agctccaggg tgatggcctc
     6721 atcaattttc gggcattcgc gataacaaac atcgcagcgc agcccctgaa agttgagaca
     6781 gttttcctgg tccaccagta ccgccagccc cattcgcgcg tcgtcgatcg attcgatctc
     6841 acgatccagc gcaccgctcg ggcacacttt ggcgcacgga atgtcctcac acatttcgca
     6901 aggaatatcc cgtgcgacaa aatatggcgt gcccgccgac aggccagagg ccagcgtcgc
     6961 cagttttaag gtgtcgtaag ggcaagcctg aacacactga ccgcaacgca cacaggcact
     7021 ggcaaaggcg ttctcgttta tggctccggg cgggcgcaac cgcacgccag atgcgcgtgc
     7081 ggtttgctgt tgtaacccca gcgccacacc cacggcagcc agcccgccag ctgtgcgaac
     7141 gacatcgcgc agaaagcggc ggcgaccatt ttgaggtttc gctgaccggg acataatggc
     7201 ttacaccttc tccagtttga ccgcgcactt cttgaagtcc gtctctttcg agagcggatc
     7261 ggtcgcatcc agcgtcagtt tgttaaccag ctgtgcggca tcgaagaacg gcatgtacac
     7321 caggccctgc ggcggacggt tacgaccgcg cgtttcgaca atcgagatca cttcgccacg
     7381 gcgagaaacc actttcactt tgtcgccacg gcgcagatcg cgcgctttcg catccagcgg
     7441 gtgaataaac aggaccgctt ccgggaaggc gcggtgcagt tccggtacac ggcgagtcat
     7501 actgccggta tgccagtgct ccaggacgcg tccggtagag agccacaggt cgtactcttc
     7561 atccggtgct tccgccgcag gttcgaacgg cagcgcgaag atcaccgctt tgccatccgg
     7621 tttaccgtag aacttgtagc cttcgcccgc tttcacgtac gggtcgttac cttcgctgta
     7681 acgccactgc gtttctttac cgttcaccac cggccagcgc agaccgcgtg ctttgtggta
     7741 gtcatcgaac ggcgcgagat cgtgaccgtg accgcgacca aaccatgcgt actcttcgaa
     7801 cagccctttt tgcagataga agcccagctc gcgggattca tcgttcagct gatcttccgc
     7861 cagttcggat agcgggaatt tgctcacttc cggggtggca tacagaactt cgtacagcgt
     7921 tttgccacgc agttccggtt tcttcgccag cagctcttcc ggccatactt cttcagtttt
     7981 gaagcggcgg gagaactgga ctaactgcca gagatccgat ttcgcttcgc ccggtgcctg
     8041 tacctgctga cgccagaatt gagtacggcg ttcggcgtta ccgtaagccc cctctttctc
     8101 tacccacatt gcggtcggca ggatcaagtc cgctgccagc gcactgactg tcggatacgg
     8161 atcggaaacg atgataaagt tgcgcggatc gcgccagcct ggcatacgct cttcattaat
     8221 gttcggcccg gcctgcatgt tgttggtaca catggtccag taaacattca gcttgccgtc
     8281 tttcagcgca cggtcttgtg ccaccgcgtg cagaccgatt ttcgccggaa tggtgccact
     8341 cgggatattc cacttcttct cgcaaatatc acgatgtttc tcgttagtca ccaccatgtc
     8401 cgcaggcaga cggtgagcaa aggtgcccac ttcacgcgca gtaccacacg cggaaggctg
     8461 cccggtcagg gagaacggac cgcaacccgg ctgggaaatt ttgccggtca gcaggtgcag
     8521 gttgtagacc aggttgttgg cccacacacc acgggtatgc tggttgaagc ccatcgtcca
     8581 gtaggagatg actttcttgt tcggatcggc atacagctgc gccagttgtt ctaactggtc
     8641 tttcggcacg ccggtcattt cggcggtttt ttccagcgta tattcagcaa cgaaggcttt
     8701 gtaatcttca aagctcatcg gttcggaggc gtcagaaccc ggattcttcg ctgctttttc
     8761 cagcggatgg gtcggacgta aaccgtagcc gatgtccgtc gcccctttgc gcaggttaac
     8821 gtgtttgctg aagaagtcct gatttatcgc attgttttga atgatatagt tggcgatata
     8881 gttcaggatc accaggtcag attgcggcgt aaagatgatg ccgttatccg ccagctcgaa
     8941 gctacgatgc tggtaggtag aaagcaccgc cacggtgacg ttctggttgg agagacgacg
     9001 gttagtgatg cgcgaccaga ggatcgggtg catctccgcc atgttggagc cccacagcac
     9061 aaacgcgtca gcctgctcga tgtcgtcata gcagcccatc ggctcatcca taccaaaggt
     9121 acgcataaag ccaactaccg ccgacgccat acagtgacgc gcgttcgggt cgatgttgtt
     9181 cgaacggaat cccgctttaa acagcttgga cgcggcataa ccttcccaga tagtccactg
     9241 accagaaccg aacataccga tcgattccgg ccctttttct ttcagggcgg ttttgaattt
     9301 ctcttccatc acatcgaagg cttgatccca ggtaattggg gtaaattcgc cttctttgtc
     9361 atatttaccg tttttcatac gcagcagcgg ctgcgtcaaa cggtctttac cgtacatgat
     9421 tttgggcagg aaatagccct taatgcagtt cagaccacgg ttaaccggtg cgtccgggtc
     9481 gccctgacag gccaccacac gcccctgctg cgttccgacc agaacgccgc aacccgtacc
     9541 gcagaaacgg cacggcgctt tatcccattt aatggcttcc tgctgaccaa caacggcgcg
     9601 ggcaacgccc ggcacgctga gaccggcagc cgccgcagcg gccgcaacgg cgttagcttt
     9661 cataaagcta cgacgactga gtttcatggt gtttcctcac cttgctcttc ctgctggtga
     9721 taaaccagcg acaccgccag cacgccctct acgttgcgta ctgactcaat ggtttggatc
     9781 agcgtttcgc tgtcttctgc ttccaccacc acaatcaact ggccgctcgg cgcgtcgctg
     9841 acagcaactt cacagccggg aaaggcgttc agttgggtgc tgatgtctga aattcgttcg
     9901 cttttggcct gcacgaccag gctgcaaact tgccagttag tgtacatgga gatactccgc
     9961 agttatggct gataccggac agctggctgc acatgctcca catgcgttgc aaagttggct
    10021 attaagttgc ggctggtaga tcccggagag cgtcgggcga aagataattg ccattggctc
    10081 acagctatcc tgacagcggc ggcattcaac tgactgatac gccaggcacg cctccccgat
    10141 ggtgaacagc aaatcccagg ccctggtgtg gcgcggagaa aatagcgatt cggggcaagc
    10201 ctgcgcgcag gcgtaacaga agctgcactc gttatttttg aaattaacgc tcggatagcc
    10261 acccgcgccg cgttgcagaa tgttgttttc acaagcatta atacaggcgt cgcagcgagt
    10321 gcaatgggtc agaaaatgag attcatcacc ggaccagggc ggacggatac cgttactggc
    10381 tttgcgccag cgaccagtga gtatgccccg acgggatgca tcaatcttca cattgacctt
    10441 ccatcattaa cgcgctctga aaattgagag cgaccaaata aaccgcataa ttaataagcc
    10501 atttttatag ccgctaagat attaaaggat gtgtcaaaga tgcatacccc gatcggggta
    10561 aaacctgtag caggatcaaa agagtggcag gaagcgtggc aaaaacgggc ttttgctcac
    10621 atttcaaatg gttataaaca tatttatata gcgattaatt cgccagagat atttctgctg
    10681 gtttgttttc tcatcagaat ttaacactaa aagagcaggt aaaattatct gaatgttctt
    10741 taagttattc ataaagcaaa ttaataaatc tgatgaatat gttaaccttc agcgacatca
    10801 tcggtgaaaa cctataaatg aagaaggaaa gcaaaaaaat gaagaccatt ctacctgcag
    10861 tattgtttgc cgctttcgcc accacttccg cctgggcggc agaaagcgtc cagccactgg
    10921 aaaaaatcgc accttatcca caagctgaaa aaggcatgaa gcgccaggtg attcagttaa
    10981 ccccgcaaga agatgaatct accctgaaag tagaactgtt aatcggccag acgctggaag
    11041 tcgattgcaa tttgcatcgt ctcggcggga agctggaaag caaaacgctg gaaggctggg
    11101 gctatgatta ttatgtcttt gataaagtca gttccccggt ttcgacgatg atggcctgcc
    11161 cggatggcaa gaaagagaag aaatttgtca ccgcgtatct gggcgatgcc ggaatgctgc
    11221 gttataacag caagctgccg atcgtggtgt atacgccaga caatgtagat gtgaagtacc
    11281 gcgtctggaa ggcggaagag aaaattgaca acgcggtagt tcgctaaact gctgtgaagt
    11341 gctgcaaccg ccaggtcgga taaggcggtt acgccgcatc cgacatctaa cgcccgagcc
    11401 ggttgcctga tgcgacgctg acgcgtctta tctgcatacg ccacatccgg cataccatgc
    11461 cggatgtggc acatcatcac aacgcaatat ccgccacttc tttttgaacg ggttgcggtt
    11521 tcaactgcgg tttcggcgta ctatctgctg cctgcggttt gtcgtatttc agccccagca
    11581 cttcgctggt gtactgcaac tcgcgttctg ttgccgctac atcaccgttc agcttgcgtc
    11641 cataagacgg aacgatcgct ttcaacgtag cctgccattg cgggctggaa acgcgatcgc
    11701 caaatacttt ttccagcaga tccaacataa tcggcgcggc ggttgacgcc cctggcgatg
    11761 cccccagcag cgcggcaatg gttccttgtt ggtcgctgac cacttcggta cccagacgca
    11821 gtacgccacc tttctcggca tcacgcttga taatctgcac gcgctgcccc gcttgccaca
    11881 aacgccagtc ctcttttttc gcctgcggat agtactcttt caacgcttca aaacgatcct
    11941 cttcactcaa catcacctga ctcaccagat atttcaccag atcgaaatta tccagcccga
    12001 cgtgcatcat cggcatcacg ttagaggtgg tggtggaact catcagatcc cacaatgaac
    12061 cgtttttgag gaatttggtt gagaaggtgg caaatggccc aaacagcacg acgcgtttac
    12121 cgtccagaac acgggtatcg atatgcggaa ccgacatcgg tggtgcgcca acggatgctt
    12181 taccgtaaac cttcgccaga tggtgattaa ccacgtccgg gttttccgaa acaaggaact
    12241 gtccgcccac cgggaaaccg gcgtagtctt tcgcttccgg aatccccgat tcctgtaaca
    12301 gcttcagcgc cgcaccgccc gcgccgataa agacaaattt ggcgcggatg ttctgtgcag
    12361 tgccattttt cagatcggca acagtgacgg tccaggtgtt atcgtcatta cgctttaggg
    12421 cgcggacttc gctgctgagt tgcagcgaaa aattagattt cttctgcaag gaagcaatta
    12481 actggcgggt gatctcgcca tagttcacat cagtaccaat ttccgtacgc gtggctgcca
    12541 ctttctgttg cggatcgcgc ccttccatca ctaacggtgc ccactctttg atctgcgcgt
    12601 gatcttcaga gtaacgcata ccgcgaaaca gcgagctttg ttgcaacgcg gcgtaacggg
    12661 cgcgcaggaa attgacgtta tcctcgcccc agacaaagct catatgcgga acggtattga
    12721 taaatgaacg cggagtacgc agcacgccgc gctcgacctg gtgcgcccag aactggcgcg
    12781 aaatctgaaa tgcttcgtta atggcgactg ctttttcaat actgatgctg ccatcggcgt
    12841 tttgcggagt gtaattgagt tccatcagtg cagaatgccc ggttccggcg ttgttccagc
    12901 cgttcgaact ctcctgcgcg acaccctcca ggcgctccac catggtcatc gaccattcag
    12961 gctccagctc gcgtaaatag gtccccaacg tggcgctcat aatgccgccg ccaatcaaca
    13021 gtacatcagt ttcctgctcc tcggatgctt tcgcttttgc cgccatcgaa acggcattaa
    13081 gccccacggc catcgagaag agcatggcag tcactttttt catcttgtta atgccttact
    13141 tttagtcgct ttattgcagg tgagatttgc gcggcatcaa cggtaacact taagtaacaa
    13201 catttaaata atgtttataa attatattct aattttagaa acatt
//
LOCUS       DAZGHF010000129        13197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGHF010000129 DAZGHF010000000
VERSION     DAZGHF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.130"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            39..1763
                     /locus_tag="ACHK3A_003941"
     CDS             39..1763
                     /locus_tag="ACHK3A_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO8741966.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKARSEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKA
                     LPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPGDR
                     CVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPP
                     FGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPC
                     YGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEH
                     GIEIRNEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDG
                     YLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRIS
                     DEERRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAAS
                     LHVQESLA"
     gene            1760..2674
                     /locus_tag="ACHK3A_003942"
     CDS             1760..2674
                     /locus_tag="ACHK3A_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO8741967.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     IWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLIAMADEITLGIFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLNAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAEARCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SANNNRISEDINWFPGISLEHALRNGLF"
     gene            2706..2954
                     /locus_tag="ACHK3A_003943"
     CDS             2706..2954
                     /locus_tag="ACHK3A_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO8741968.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2954..4126
                     /locus_tag="ACHK3A_003944"
     CDS             2954..4126
                     /locus_tag="ACHK3A_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO8741969.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTSYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTISTQPELRQKLMDNANNLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4161..5240)
                     /gene="lptG"
                     /locus_tag="ACHK3A_003945"
     CDS             complement(4161..5240)
                     /gene="lptG"
                     /locus_tag="ACHK3A_003945"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO8741970.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVDVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETQ
                     ETSDNLPWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5237..6307)
                     /gene="lptF"
                     /locus_tag="ACHK3A_003946"
     CDS             complement(5237..6307)
                     /gene="lptF"
                     /locus_tag="ACHK3A_003946"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO8741971.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6337..6897)
                     /locus_tag="ACHK3A_003947"
     CDS             complement(6337..6897)
                     /locus_tag="ACHK3A_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO8741972.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLRNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6909..7733)
                     /locus_tag="ACHK3A_003948"
     CDS             complement(6909..7733)
                     /locus_tag="ACHK3A_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741973.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPV"
     gene            complement(7733..9082)
                     /locus_tag="ACHK3A_003949"
     CDS             complement(7733..9082)
                     /locus_tag="ACHK3A_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO8741974.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYASNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9128..9886)
                     /locus_tag="ACHK3A_003950"
     CDS             complement(9128..9886)
                     /locus_tag="ACHK3A_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741975.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9918..10631)
                     /gene="yghS"
                     /locus_tag="ACHK3A_003951"
     CDS             complement(9918..10631)
                     /gene="yghS"
                     /locus_tag="ACHK3A_003951"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO8741976.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            10805..11497
                     /gene="yghT"
                     /locus_tag="ACHK3A_003952"
     CDS             10805..11497
                     /gene="yghT"
                     /locus_tag="ACHK3A_003952"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO8741977.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIEKNEVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11546..13045)
                     /gene="pitB"
                     /locus_tag="ACHK3A_003953"
     CDS             complement(11546..13045)
                     /gene="pitB"
                     /locus_tag="ACHK3A_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO8741978.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            12982..>13197
                     /locus_tag="ACHK3A_003954"
     CDS             12982..>13197
                     /locus_tag="ACHK3A_003954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8741979.1"
                     /translation="MPEQVITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYLFLTIKRVLFLVYQTISIHSKSLEI"
BASE COUNT         3350 a         3419 c         3256 g         3172 t
ORIGIN      
        1 tcattttatt ttggatatcc aattttttcg tggtgtatat gtctaataaa atctttacgc
       61 attccctacc tatgcgctat gccgattttc caacgctggt tgatgctttg gactacgccg
      121 ctctgagtag cgccggaatg aatttttatg acagacgttg ccaacttgaa gatcaactgg
      181 aatatcagac gttaaaagcg cgttccgaag ctggtgcgaa aaggttgtta tcgctgaacc
      241 tgaaaaaagg cgatcgcgtg gcgctgattg ccgaaacgag tagcgggttt gtagaggctt
      301 tttttgcctg ccagtatgcc ggcttagtcg ccgtcccgtt ggcgattcca atgggcgtcg
      361 gtcagcggga ttcctggagc gccaaactgc agggtttact ggcaagttgc cagcccgcag
      421 ccattatcac tggtgatgag tggttgccac tggtcaatgc cgcgacgcat aacaacaacc
      481 ccgaattaca tgttttaagc cacgcctggt ttaaggcatt accggaagcc gatgttgcgc
      541 tccagcgtcc agttccaaac gatatcgcct acctccagta cacctccggc agcacccgtt
      601 ttccccgtgg cgtcattatc acccatcacg aagtgatggc taatctacgt gctataagcc
      661 acgatggcat taaattacgc cctggcgacc gctgcgtctc ctggctgcct ttctaccatg
      721 atatgggact ggtcggcttt ctcctgaccc ccgtcgccac gcagctttca gtagattatt
      781 tgcgcactca ggattttgcc atgcgtcctc tgcaatggct taaattgatc agtaaaaatc
      841 gcggcaccgt ttccgttgca ccgccgtttg gctatgaatt gtgccagcgc cgcgtgaatg
      901 aaaaagatct cgctgaactg gatctttcct gctggcgcgt cgctggtatt ggagcagaac
      961 cgatctccgc agaacaactc catcaattcg ctgaatgttt tcgtcaggtt aactttgacg
     1021 ataaaacttt catgccgtgc tacggactgg cagaaaatgc gctggctgtc agcttctctg
     1081 atgaagcctg cggggttgtg gttaacgaag tggatcgcga catcctcgaa taccagggta
     1141 aagccgtcgc gccgggtgct gaaacacgcg ccgtatcgac tttcgtcaac tgcggtaaag
     1201 cgttgccgga acatggcatt gaaatccgca atgaagcaga tatacctgtc gcggaacgtg
     1261 tggtaggcca tatttgcatc tccggcccca gcctgatgag cgggtacttt ggcgaccagg
     1321 tttcgcaaga cgagattgcc gcgacgggct ggttagacac cggcgacctt ggttatctgc
     1381 tggacggtta tctgtatgtc accggacgca ttaaagatct gattattatt cgtggccgta
     1441 atatctggcc gcaggatatt gaatacattg cggaacagga accggaaatt cattctggcg
     1501 atgcgattgc ttttgttacc gcccaggaaa aaatcatttt gcagatccag tgtcggatca
     1561 gcgacgaaga acgtcgcggg cagcttatcc acgcgctggc agctcggatc caaagcgaat
     1621 ttggcgttac cgcggatatc gatctgttgc cgccccacag tattccccga acatcctccg
     1681 gcaagcctgc ccgtgcggaa gcgaaaaaac gttatcagaa ggcttatgct gccagtcttc
     1741 atgtgcagga atccctggca tgaatcaaac tgtcgcggtg acgggcgcaa ccgggtttat
     1801 cggtaaatat attattgata acctgctcgc ccgcggcttt cacgttcgcg cattaacgcg
     1861 tactgcccgc gctcacgtca atgataatct catctgggtg cgcggttcgc tggaagatac
     1921 acattcgctt agcgaactgg ttgccggagc cagcgcggtg gtccattgcg ccggacaagt
     1981 gcgcggacac aaagaagaga tttttacccg ctgtaacgtt gacggcagcc tgcgcctgat
     2041 gcaagcggca aaagagagcg gcttttgcca acgttttctg tttatctctt cgctggcggc
     2101 gcgccatccc gaactctcct ggtacgcaaa ttccaaacac gtcgccgaac aacgactgat
     2161 cgcaatggct gacgaaatta cgctgggcat ttttcgcccg acagccgtgt atggtcccgg
     2221 cgataaagag ttaaaaccgc tgtttgactg gatgctgcgc ggcctgctgc cacgacttgg
     2281 tgcgccagat acacaactct ctttcctgca cgtcaccgat tttgcgcaag cagtgggtca
     2341 gtggttaaac gccgaaactg tacagacgca aacctatgaa ttatgcgatg gcgtcgctgg
     2401 cggctatgac tggcaacgcg tacgacagct tgccgccgaa gcccgttgtg gttctgtgcg
     2461 gatggttggc attcctctgc cgctactcac ctgccttgcg gatatcagta ctgcgttgag
     2521 tcgcctggca ggtaaagaac ctatgctgac ccgctcgaaa attcgtgaat taacccacgc
     2581 cgactggtcg gcaaataata accgtatttc tgaagatatt aattggtttc ccgggattag
     2641 cctggaacac gcattacgca acgggctatt ttgattgtct tgtttaaagg tgtgttaacg
     2701 tttctatggt aaatcgtgaa atagtaatgg attacatcct ttcctgttta caggatttgg
     2761 tcgaaaacgg tgtggaaatt aagcccgaca gcgatctcgt caacgatctt ggcctggagt
     2821 cgatcaaagt gatggatctc ttaatgatgc tggaagacag attcgatatt tccattccta
     2881 ttaatattct gctggatgtc aaaacccctg cgcaattaat ggaaactcta cttccatggc
     2941 tggagaataa ataatggggc tatacgataa atatgcgcgc cttgctggcg agcgcctgca
     3001 atttagtgac aacgggttaa caccatttgg cacctgtatt gatgaagtgt attccgccac
     3061 cgaaggccgg atcggcaata aaaaagtgat tctggccggg accaataatt atctcggttt
     3121 aacctttaat catgatgcta tccgcgaagg tcaggcagcg ttagcggccc aaggcactgg
     3181 caccaccggt tctcgtatgg caaacggcag ttatgcgccg catctggcgt tagagaaaga
     3241 gattgccgag tttttcaatc gccctaccgc tatcgttttt tcaaccagtt ataccgctaa
     3301 tttaggtgtc atcagcgcac ttgccgacca taacgccgtg gtgttactgg atgccgacag
     3361 ccacgccagt atttatgacg cctgttctct cggcggcgca gagattattc gtttccgcca
     3421 taatgacgtc aacgatctgg aacggcgcat ggtccgcctc ggtgaacggg cgaaagaggc
     3481 gattattatc gttgaaggca tctacagtat gctcggtgac gttgcgccgc tggcagaaat
     3541 tgttgatatc aaacgtcgtc tcggtggtta tttgattgtc gatgaagcac attcgtttgg
     3601 cgtgttaggc gcaacaggcc gcggactggt tgaagccgtt ggcgttgaag acgacgtgga
     3661 tattatcgtc ggcaccttca gtaaaagttt ggcttccatt ggtggttttg ccgtgggttc
     3721 agaagcgatg gaagtgctgc gctacggcag ccgcccgtat atttttaccg cctctccttc
     3781 tccgtcctgc attgcgaccg tgcgctcgtc gttaagaacc atttccacac aaccagagct
     3841 gcggcaaaaa ctgatggata acgccaacaa tctgtatgac ggtctgcaaa aactgggtta
     3901 tgagttaagt tcacatatta gcccggtagt gccagtaata atcggttcga aagaggaagg
     3961 tttacgcatc tggcgcgagc ttatttcgct gggcgtttat gtgaatctta tcctgccacc
     4021 tgccgcgcct gcaggcatca cgctgctgcg ctgtagcgta aacgctgccc acagtcgtga
     4081 gcaaatcgat gccatcattc aggcatttgc tactctgaag cagtaatgtg agcccgctgt
     4141 ggtgttgcgc cacggcgggt ttaatgttgt ctgtcatacc aatacaccag gctcaacgcc
     4201 accagcacaa acgctaccgg taaaccgagc gcggtaacag gtgcgctcaa cgcaaataac
     4261 aatcccagat tgaccatgat ttgatagctg atccaggtga gtaagccaac gatgacgcct
     4321 acagcgagac ggctacccat cccaggcgaa cgcggggcgc taaaggtaaa cggcacagcc
     4381 agcaaaatca tcgccagggt caagatgggt tgtcccagtt tttcccacag tgcaaggcgg
     4441 tattcgctgc tgggttgccc ggtttcctgc aaataatgga tgtaatgatt aagttgacgg
     4501 acagaaaaag tatttcccgg catcgataac tcttcaagat tcatactggt gaaggccgat
     4561 tgccagggaa gattatcaga tgtttcctgc gtttctttgc cattcagcca ttttttatga
     4621 ttcacaccat gcaggatcca cgttttgtcg tcttcaatgg tagcactgcg cgcataaatg
     4681 taggattcga gcgagagatc atcgcggtaa tgaaatatct ccacgtccac cggctggccc
     4741 tgctcgttca gcgatttcac cgtcacaaat tcattaccac gcctggccca cagcatattg
     4801 ccggtaatgt catcgtcctc ccccaacgca gtggcagtcg atttgatttg cagcgcctgt
     4861 tgctgcaatg gcgacgccac ccactcatct atcgcgccta atgaaacagt ccacaatatc
     4921 ccggcaacca gcgccaccag tgcaatacgg aagatagaaa accccgtgct gcgaatggcg
     4981 gtaagctcac tgtttttcga taactgcccc aggccgacaa tccctcccaa taaggcgata
     5041 aacggcgaaa gttcgaccag tgtgcgcggt aaggtcatca gcaccaccag caccgcctga
     5101 gtccagcgat aaccacccgg gctgacatca tccagttcgt taatcaggtt gaaggtggta
     5161 aaaagcggca gcaatagccc tgcggcggcg gcaaaaccga gaaagagatg acggattaaa
     5221 tagcgactga aaacattcat cgggaaaatt tctgcagcaa agagaagtcg cgtgcgacca
     5281 acatcagcag gcccattagc atgagtccgg gtactaacca caaaccagga atgaggggaa
     5341 tcgcgccgtt agccaccagc gtacggcaga cgtcgccgcc ataaaaaatg gcaacaaaca
     5401 gcaacgtcag tggcaataac gttgaaaatc gcccttgccg cggttttacc cgacttaatg
     5461 aaatggccag cagcgccatc aacaatgctg tcaggccacg gctttgtcgc cattgcagtt
     5521 cggcatgatc ggcaggaaag gcggagcgcg ccagctccgt gaccgatttt gctttgcgtt
     5581 tgacgttagg gctttgatcc agcggattca gatgtaattg cagattacgg tagatctgtt
     5641 cgttgtcgtc acgcccctga tgatcgagaa gataggcggt cccgttatgc aacataacgg
     5701 tcggtttctc aggtgatggg tcaaccacat caaccgaacg ggcgcggaaa atgcgggttc
     5761 gattagcagt agaagtataa atcagcgcat cagtcaggcg attattatcc tgatcaaccg
     5821 tctgcgaaag gatcattcgt ccgttatcgt tagtgttaaa tttctttgcc cgcaactggc
     5881 gaacatccag ctccgactgt gactgttgct ccagttgata aatctgcgca taagcccacg
     5941 gacgaccata catcgacaga agggtgacaa aaatacttag cggtatcgcc agataaagaa
     6001 cggctttgta caagcgtccc ggactgcccc ccgccgcaga gatagcggta atttccgagt
     6061 cggtatacat ctgccctagc gtcacgccaa ctgacacata cagtccaaca ggtaacaaca
     6121 tctccagtgc aatcagcact ttgtagaaaa cgatatccag cacaacatca agagctaatg
     6181 tcccatttgc cgcttcggtc agataacgct gtgcggagta gctggcgaaa ataaagatca
     6241 gaaaaccgac gataatcagc accagacggc gcgtaccacg catgatgtag tgttcaacca
     6301 gcttcatctc actaacgccc tcaatccttt gaatttttat tcattgatca gcttctgcgc
     6361 cagcgcaatg acatgatcta cctcttccgg cgtcaggctg ccgtcctttg cccactgaat
     6421 ttgcccggtt ttattcagca ccagaatggt tgaactctgc tcctgcaagt gccaggcaac
     6481 gcgtccctgt ccattgccgt caataacgaa ttgcgcccat ggatagcggc gtttattttt
     6541 ttcgattttg ccaacgacaa aatagccggt accaaagatg gcatcatcag tgttcacaat
     6601 ggtggtgggc tgaaaacgat cctgcggaaa gtttgccgct tcaactgctt taattaacag
     6661 tgagttcttt ttcttcgccg acttacgccc ggcgatatac tgaataatgc gaacttttcc
     6721 cgccaattgg gtgctgttcc acttttggta gtgaatttcg tcgtcgctat tccgaagcag
     6781 ctcgccgcct tcctggatat aaacgggggt aaccgttttg ccagtgacga aattgtgtgc
     6841 ctgcacaccc ggggctaaga gcatgataat cagcgcaata atcaaacgag atgacatcat
     6901 tgctccggtt ataccggaaa acgcgaaatc agcagttcta agcgctggcg aatatcgcta
     6961 tccagcgcca tcggtgcatc atacgactgg cggttttcca tatcatccgc caccatgtcg
     7021 tcgtgtttat cccccatcag gccattaatg gcggcgagga agcgggaaat ctgtaacgct
     7081 tctttatttt caggatcaag cgatcttagg gtgtcgtaat aagaaacctg ctggctgatg
     7141 atttttcgca catcatgaaa ttccctgccg gttagtcgcg gtttggcaat tatctctttc
     7201 agggtatgca tttgcttttg ccgataggca ataaaaccgc ttacgctttc taactggcta
     7261 tggcgtaatg aatagttcac caacgaccag accggagagc ttaaataaac ccgtaacagg
     7321 ttgccgtaat acgacacaat atttttctta ccgttacgaa agccgtcctg aaaacgcccc
     7381 aaaaaaacgg tcgttttgcc gaataaaaat ccggcctgat gcttctttaa atacagccgt
     7441 tgcgcaaaac gcaggtgctt gtagcgcgct cgcatgtatt taaattgttt gcgctcatca
     7501 gcagtcagct catcgccttt ggcctgtttg catattaaac gcagtaactc ctggcgattg
     7561 acgccattga gccagaattg caggcataac gcgtaattat catggatgat ttcggtggta
     7621 caacttagtg cgatagtcgg cggcaaactg acctgtgcat caacgacatc atcttcttca
     7681 atcgcaacaa atagacgctc gctcgcctct agcgggaaat attttctcat catcaggatt
     7741 tctctccggc tgcgttataa agcaaggaat ggcgctgaaa aaatgccttt aaggtcggaa
     7801 ttagcgaaag caccacatcc agacattgac caccgaaata agcaaacgct gctcccagca
     7861 cgccaaacag atgacagaac atgaacagca atacgatgta gccgattgag gcgatggttt
     7921 gcgccacgag aaaagcgcgc tgttttcccg ccatcaatag caatgactcc tgagggaagc
     7981 ccagcatcga gatcacaatt gcgcccagca tcacctgaat caggtcatac gcttcgagat
     8041 acttaacgcc aaacaccagc gaaatgagcg gcttgccgac aatcaacacc gcgagcgcca
     8101 ccagaatacc gattccgccc gccagtaaac ctgacttcac gcctaacaac cacggtctgg
     8161 tggtacgcgg atctaaacgc atcacctctg ggtaaaaact tttgcccagt aaacccgctg
     8221 gcgttccggc ggcgtcgaag aatgtcatgg cgattttaaa taacccggcg gcagcgggtc
     8281 ctaacacgat ccccaccaac actgtgctgc acgaattacg cgccgaccag atggaatggg
     8341 caatgttggt tgaccagaca aaactccacg cgcctttgat gtgtcgggca gactcaaaca
     8401 gattcaattt gaacgcgtta tggatatttc tgcggcgtaa ttcacgtgcg gcaaaccacc
     8461 agtacatggt gccgccaaca agattcgagg cgtaccaggc aataacaaaa cccgcaaaac
     8521 caaagtcaaa ataccaggca acgacgctcc ccgctgcgcg cagaaaaggt ttcgtcgctt
     8581 gctgtacagc aattagatcg aagcgatcta ccgcacgcag aatgccggtc ggcgtggagg
     8641 aagccattga aggaatgagc gtgcaataca gcgctgccag ccaaaagctt tggtcatcca
     8701 gacctaatga atgggaaaga aaagggagta aggcaatgcc gccgacaatc gccaccgcgc
     8761 cgctgacgat atccagcgag aaggaaaatg agacgacatt gcggaattgc tgcggattat
     8821 tgttggttaa tgctggtgtt ccgtactgaa ccaccagctg ccatgtctga aacttaataa
     8881 aatcgctgat cgacttggcg tacgattgta caatcaccag tacgccaaac atggcgggcg
     8941 tcatcccttt accggcacac gagagcgcca acagacccag caaagcgctc acgacattac
     9001 tggagcctaa ccaggcgcta ttgcgaataa tggtgcgaaa cgcgccatct gcaaaccaat
     9061 gtttgatgtt aaaacccgcc aattcagcct gaccttatcg ttgatagtaa aaagtatccc
     9121 gccagcctta agttaaactt cggcggtcag aaacgatggc aaccagagaa accgccttct
     9181 gtgcctgttc cagcacttcg ctgtagggcg ctctggaatc aatctcaaga atttttgtgc
     9241 cgttatagcc aatcgtcgac atgacgccga ttttgtcctg cagctcggca tagtcatggt
     9301 caggcttgcg ggagatggca gtatcaatat caatccccaa acgaatgatt aattcaggac
     9361 ggtattgcgc catttgttgg tataaacgcc gttcgcgctg cgccagaaac atgctgattt
     9421 tcccggtcgc acgttcgacg ccgatccccg gtccgtcata ataaaagccc gaaatttcag
     9481 cctgcgggaa gcgatcgctg accaccagaa cgccactttg cgccagtcgc tgaaccttgc
     9541 gtagattcgc cattcttcgc agcgagaagc agtacataat caccgccgcc catagcgctg
     9601 gagatttggt tttcatgctt tgggttttcg aggatttggc cgccagtcgc cgttcgagcc
     9661 agacgccaac caacggcaat cgtttgattt tgtcgccgtc ttcgcccgag agcagcccca
     9721 gatagcgccg ctcggtttgc cagtgttgtt gcagcgattt caccaggtcg gtggtcagtg
     9781 tggatttacc ggtgccatca cacccgacca ccgcaatcag ccccggaatg tagttgggct
     9841 gcggcgcggt tgtactatta acagtttgtg tttgtagtgc gtccattcgt taaatcatcc
     9901 cctatccatt ttggtgatca ggaaagcgcg gcgtgaattg cgcgtagtga cgcttgcaga
     9961 atttcatcgg cgggttgccg accatccagt tcaaggatct ttgcgccatt aaatgtcagt
    10021 tgcggcgtaa cggcgatttt ttcctgtaac gccgccagtt ggtggtcagg tttacgcgca
    10081 aacgcggttt gttcatcaat gccaagacga atcaacaata cgggcaaata agacgccatc
    10141 cattggtaca gcttcagctc gcgctgcctt aacattttta tccaaccgtt accgcccgtg
    10201 gtttttgcca attgcgggcc atcaaagcga aaacccggca cttcaacctg cgggtagcgg
    10261 tcggtgatga gcagatagcc ttgctggctt ttacacaaca ttttgcgaaa cttgtacgcc
    10321 cgccagcagg aaagcagata gataactagc gcagtaatat tgccaggtgg tgtggagggc
    10381 ttttcgtgca catgtgccgc tttacttcgc agataacgcc caaaaggtgc gccaataaca
    10441 gggagctgtg aaatccattc gccaattcgc ccggacgatt gcccgagata aatgtgttct
    10501 gttggcattg ttgctgccag ttcatttacc aggcttgccg tgagggtcga tttacctgaa
    10561 ccatcacatc ctacaatggc aataacacgc actggcgtgg aattaattat tgacatatta
    10621 cgttgattca cgaaaaaccc ggcagcaaat aatgtattga atattacgtg gtccgtgctc
    10681 agaatatccg ttcaacctta ttgtgacaat ggttaattgt gacattaacc tgaattacgc
    10741 tttcataaaa acatattaat caaataaata ttttggatgg atatttaaat ttaaggatat
    10801 attcatgcag tcaataacac ctccattaat tgccgttatt ggtagcgatg gttcaggcaa
    10861 gtcaacggtg tgtgaacatc ttattaccgt tgtcgaaaaa tatggtgctg ccgaaagagt
    10921 tcatttagga aaacaggccg gaaatgtcgg tcgtgcagtg acaaaattac cgttgatggg
    10981 aaagtctctg cacaagacta ttgaaaaaaa tgaagtgaaa acggcaaaaa aattgccggg
    11041 tccggttccg gccctggtga tcaccgcttt cgtcgcccgc cgactgttac gctttcgtca
    11101 tatgctcgcc tgtcgtcgtc gcgggctgat tgtcttaacc gatcgttatc ctcaggacca
    11161 aattcctggc gcttacgatg gtacggtgtt cccacctaac gttgaaggcg gccggtttgt
    11221 ctcatggctg gcaagccagg aacgtaaagc atttcactgg atggcgagcc ataagcctga
    11281 tctggtcatc aaactcaatg ttgaccttga agttgcctgc gcgcgtaaac ccgaccataa
    11341 acgggaatcg ctggcgagga agattgccat aacgccacag ttaacctttg gcggtgcaca
    11401 actggttgat atcgacgcca atcagccact ggaaaaggtg ctggttgatg cagaaaaagc
    11461 gattacggat tttatgaccg cgcgtggtta tcactagtca aaaatggaaa tgcccgatcg
    11521 ccaggaccgg gcattttcag gaaggttaaa tcaactgcaa tgctatccag tacaacccac
    11581 cagaaagaaa aatcgccgcc ggtaaagtaa acacccacgc catcaggatg ctggttaccg
    11641 ttttacgctg taacccaccg ccgtccacca ccatcgtccc tgctaccgca gacgagagga
    11701 cgtgtgttgt ggagacgggc atcccaatat aactggcaag gccgatagac actgccgccg
    11761 tcatttgtgc cgccatgcct tgcgcatacg tcatgccgcg cttaccaatc ttctcaccga
    11821 tggtcatcgc tacacgacgc cagccaatca tggtgccaat gccgagcgcc agagctactg
    11881 ccatgatgat ccacaccgga gcgtactcaa tggtgcttaa catatcgctg cgcagttttt
    11941 tcagcaggtt ctggtcttct ttactgacgc ctggcagttt cgccagcttc gcggaggtat
    12001 cagagatgca cagcataatg cggcgcagct ggctgcgctg actcacgctt aacggctcgt
    12061 aactttccat attgcctggc agcatcgttt taacgcgcgc aatcgcatca aaggtatttg
    12121 ccggatgaca gtgaaactcc gttacttgcg tgccatcagt cgatgccgca ggcaatggag
    12181 gttccatcgc aatcaacttc tgcggcagtt caggatgctg ttgcaggtag tgttcgaagt
    12241 tggtgacggc atcgcgggta cgggtaattt catagccgga cgcattcata ttgacgacga
    12301 aaccagcagg ggcaatcccc accagtacca gcatcaccag gccgatccct ttctgcccgt
    12361 cgttagcacc gtgcgaaaac gccacgcccg cagcggaaac aatcagcgca atacgcgtcc
    12421 agaatggtgg cttacgtttg ccttttttct ttttgcgatc ttccggaatg cggtgaatac
    12481 ggtcacgctt tttcgtcccg ctccagaaac ggcgcagcag gaaaatcaga ccacccgcaa
    12541 tgaccaggcc gacgataggg gaaacaatca gcgaggagaa aattttggtc acttcacgca
    12601 ggtttaacgc atccatcacc gatgagccgg ttaacagcgc gttggttaaa ccgatgccga
    12661 taatcgcacc aatcaaggtg tgcgaactgg aggccggtaa accaaagaac cacgttccca
    12721 ggttccagat aatcgcggcc agcagcatgg aaaagaccat cgccaggccg tgagttgacc
    12781 ccatattcag caacaaatcg gttggtaaca tatggacaat ggcataggca acgctaagtc
    12841 cgcctagcaa tacgccaaaa aagttaaaaa atgccgccat caccacggca agctgtggct
    12901 gcatggctcg agtataaata acggttgcca ccgcattcgc cgtgtcatga aaaccattga
    12961 ttgcttcgta gaacaacaca aatgccagag caagtaataa caaaagccct gtgtatatat
    13021 caaggccaac aaataaattt agcatatatt agattacgcc attttgaatt tacgaacgga
    13081 cgcattatca gtgactttaa cggcatgggc aaagtgaaat atcttttttt aacgataaaa
    13141 agagtgttat ttcttgtgta tcagacaata agcattcatt caaaatcttt agaaata
//
LOCUS       DAZGHF010000130        13149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGHF010000130 DAZGHF010000000
VERSION     DAZGHF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.131"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..821
                     /locus_tag="ACHK3A_003955"
     CDS             <1..821
                     /locus_tag="ACHK3A_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO8741980.1"
                     /translation="TVAVLVPSLTDKTSSRFMQSLQQVLNKNEFQLLLGCHEYNQLKE
                     AEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPFSPAQITIETAFFE
                     ASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSYYQNSDLVITMPGAAS
                     LQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSDSC
                     DQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            1024..1704
                     /locus_tag="ACHK3A_003956"
                     /pseudo
     CDS             1024..1704
                     /locus_tag="ACHK3A_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1721..2029
                     /locus_tag="ACHK3A_003957"
                     /pseudo
     CDS             1721..2029
                     /locus_tag="ACHK3A_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2132..3238)
                     /locus_tag="ACHK3A_003958"
                     /pseudo
     CDS             complement(2132..3238)
                     /locus_tag="ACHK3A_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4005..6053
                     /gene="ireA"
                     /locus_tag="ACHK3A_003959"
     CDS             4005..6053
                     /gene="ireA"
                     /locus_tag="ACHK3A_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFO8741981.1"
                     /translation="MKNKYIIAPGIAVICSTVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
     gene            6727..11043
                     /locus_tag="ACHK3A_003960"
     CDS             6727..11043
                     /locus_tag="ACHK3A_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO8741982.1"
                     /translation="MNLRKKHICIAISLVLNPFPASLAAQNTNVVEVDESSQGYDVHG
                     DNWKSIEIGTTQEVKASPDKNINFDHNSPVAEGRNSIAIGTSAEAGKEMTDKMAGEAI
                     AIGAKAKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGDRFGDKEIPNNYSSSEAS
                     GDGSIVVGSRSTAAGDLSVSLGSISSANGTAANALGATAEANGDMATAIGFNALANAS
                     NALAVGSQSSAGGEESLAIGSQSTTGKKSTVALGQGATASAAEGNVAIGADSVDKAAT
                     PVSGATIKLKNGGTIEYRGFAGDKAASVVSVGDAGKERQIVNVGAGAVSEKSTDAING
                     SQLYAIAKTLKDDLDAINPTWTLKDDKGGSATINGGKDNKNGNTLTVKGDGDIAVTVN
                     DKGMTIDGTTLKNEIKNDAIKIKGGDGKTLTKSLGDNVEITGKGPIKTQVAGDKLEIS
                     AETASLPVNGGNGKITVNNGDGDKLVTAKNIAEAINQSGWKIKADKDTGGVIEGNGQG
                     TETLIKPGDMVTLKAGKNLSVKQSGGEFTFSVIDTPSFSSVQLNGNGKNGNVTLSSEG
                     NKLTLSGGNGAGQNGVVISGVASGLEGKELSTLTNTDKEMKNAASVADLKTAIDGVST
                     SMQGAGFALKDSKGQEVKQSLGQSIKVSGDSNITTTAITNGDKGLKISLNNNLSVGSN
                     NTPGSIKVNGADGKEAVSLNGQDGTIKLTSAPAANGKSASATIAVNAGKGGLTDAAGT
                     TKTRLTYAPDGTLPAEELATMNDGLSFEGDDGKVIHKKLNEKLTIKGGAGDNEDVTDS
                     NIRVDANEKGDGLLVRMTRNLRDLASAVFGTGGQITTVNDKGITITTPAAGGGQGNTV
                     SLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDLKNALRDNSAALVNTG
                     FGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRLADDGSVSVGGT
                     RMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNVSQLNREISQATAATTW
                     ALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSYEIDVTGIPVEYTDSQGN
                     PLVNIGGRFYTQTEDAATGKLTMTPAEPARVRISSEQPLVLTNVAAGNVSPVSTDAVN
                     GSQLASAARIAGGDNVVWKDGKAALAPDTFSELTTAGGGKTTVNGGVHKTPDNVADAV
                     NMLNREGTKYFKAASSGQAAKAEGRDSIAVGQGAVSRGQNSIAMGNGAEVTRQAAAGS
                     VAVGSHARAGRANTGTYALNSQAVAGRTGSDTAVVSFGQPGQERQLQSVAPGVLSANS
                     TDAVNGSQLHATNRQVAGNTQAINTLGNKFSQLSYRVEELNRDIRGVGASAAAMSGIP
                     QAYLPGKSLMGLGVGGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNFSVGAGVGW
                     QW"
     gene            11068..11841
                     /locus_tag="ACHK3A_003961"
     CDS             11068..11841
                     /locus_tag="ACHK3A_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8741983.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGQSVR"
     gene            11992..12817
                     /locus_tag="ACHK3A_003962"
                     /pseudo
     CDS             11992..12817
                     /locus_tag="ACHK3A_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3709 a         2762 c         3373 g         3305 t
ORIGIN      
        1 atacagtcgc cgtactggtt ccttccctga cagataaaac cagctcccgc tttatgcagt
       61 cgttgcagca ggtgcttaat aaaaatgaat tccagctact gcttgggtgt catgagtata
      121 atcagctcaa agaagctgaa atcctgatga cgcttcttca aggtaacccg gcggcgttag
      181 ttatttttgg ttctcagttg gctgataaaa ctcaccagat actcgagaaa acaaatatac
      241 ctaccattaa cgttgtcggt tcccctttca gtccggctca aattaccatc gaaacagcat
      301 tttttgaagc atcacataaa ctgaccgaac atctgctgga acagggttat aagaatatcg
      361 gatttatcgg tgcgcatatg gacaaccgcc tgcaacgtca gcaactgaac ggctggcata
      421 aagcaatgct gagctattac cagaactctg atcttgttat caccatgccg ggtgccgcaa
      481 gtttgcaatt ggggcgttac gcattaaacg agatattact gcgacaacca gagctggatg
      541 ctgtgatttg cagccacgaa gagattgcgc tgggtatcat gtttgagtgc cagcggcgtt
      601 tgttaaaaat ccctggaaat atcgctgtag cttgtcttga cggttcagac agttgcgatc
      661 aaacacaccc aaccttgaca tccatccgta ttgactacaa aaaaatgggg acagagacgg
      721 gtaagctgct gattggtcta ttgaataata atcatgatga atcagaagag tcgcgtatcg
      781 tgcaattcaa ctaccagata gaattacgtc agagtacatg agctcaaaag gggattattg
      841 caaagttagc tattgtggta gggatagccg tttccggcta ttccccgcgc agagccggat
      901 gtgtgctgct aacgcatcct gtcaacgacg gatgaaaagt tatccactta tatctccacc
      961 aacggcccaa tattgtatcg ttcgtgacac atccatttct acttcaggta tttggcgtag
     1021 tagcgggtgc taaaaatttg ccgcaaaacc gcaatatcag cccgcagagg cgctctccag
     1081 attatccatt cctatattat ctctgcaccg gatgaattac tcgaacaact cagaaatatg
     1141 acacatatgc aacttatcag gactctggga tcctggaggc ctgatgccag tgaatacagt
     1201 aatgttacca acgtttatcg cattacattg aaatcccttg ttcgacgcta tttcgagttt
     1261 catgatgaaa tcaccaactt ggatgtcatg attgcggcaa ttgttgatga actggcacct
     1321 gaactgatta aacgtaatgc tgttggatac gaaagcgctt tgcagttgtt gatctctgcc
     1381 ggagacaatc cctaacggtt aagaaccgaa tcaggttttg cggcgttgcg cggtgtcagc
     1441 cagtttcccc tatcttctgg aaaaacgaac cattaccgac ttaatcgggg tggggagcgt
     1501 gttgcaaata gtgcacttta catcatcact ataggatgtt tacaaactga cgataaaaca
     1561 aaggaatatg tctccaggcg agtagccgaa ggacatacaa aaatggaagc aatacgctgt
     1621 ctgaagcgct atatctcacg cgaagtttat acattactac gaaatcaaaa caggcatgtt
     1681 aactgcatcc cgataacgtc ttgactctta gaagggcgtc caaggcagtc attattcgag
     1741 caggcagttc cggcagttat tgtgtcgata ccagatcagg cagagtatga gccggcgcgg
     1801 aaaatgctgg gataacagct caatggaacg cttcttcagg agtctgaaga acgaatggat
     1861 gccggtggtg ggttacgtaa gcttcagcga ggcagctcac gccataacgg acgatatcgt
     1921 tggatattac agcgcactaa gaccgcacga atataacagt gggttatccc caaacgaatc
     1981 ggaaaatcga tactggaaaa actctaactc ggtggccagt ttttgttgac cacttcagcc
     2041 atcgccagac gctaacatga gttactcttg ttctgggcta cgtcaccttc agggaactgg
     2101 ttatcctcat tctttatttg tgacattgtg gttattgtgc gacaaacatg gctatcccgc
     2161 cccatatccc agataaaacc acctgctcac gtgcgacagc gacaattgta atattgacat
     2221 tctttccttt ggcctgaagt tttcgagacc tcttgcaaag cctgagttga gcatcccatg
     2281 ctctgtcaat gacggggcgg ggtaaatttt cctgattttt tgtattgccg gactgatgcg
     2341 tgtcggatgc cggtaactcc acgctgcctc aaccaatagc tttctggcat agctgtttcc
     2401 ggctttggta atgctgccct gatgtctgct tccacccgat gaatattctg atggcgtgat
     2461 accaaaccat gacatcagtt gtcgtggatg ttcaaaacgg gtcagatcac ccagttcaga
     2521 aattaatcca accgcagtaa taaactgaat ccctcgcatg gcttgctgcg cctctactac
     2581 cggatacagc ctccattcgg taacggcctc cttcgggcgg attccagccg ttcacattgt
     2641 gcctgccggt cttctattgt acaacggtgt tcgtcaaaag ctaattgtcg ccagggactt
     2701 tcgaatgagt atttgctgag ccagtgtcgg tgtgcagggc cccaatctgc tcgcccgaca
     2761 taatggaccc catgaaccag aagaaacgat ttcagacgtt gccttgcatg ccgtaaatca
     2821 tcgcgggcag atgcccatgc ccgggccaaa tcccggaatg cctcatcttc aacgccggat
     2881 acgtagacag cagaaagatc tcctgcacgc agtgaacgca caagtctgat ggcatcacgg
     2941 tgtcggtttt accccgctcc cccggttttt tcgggataag tgagggggcg cagaccatgc
     3001 agtcaaaacc ggatttcacc agccgacgat aaagtccata accacagggt cccgctttat
     3061 aaacgatact gacctgcgat gactttgacc tgatacgttg ataaagatta tgaatgtcag
     3121 taggtgatgt gctaatttta cccatcagtt caacaggttc tgaattgata gcataggaga
     3181 cagtaatcga ttctttgtga acatctaatt cgacgtacaa catgttattg ttgttcatgc
     3241 tggcctccgt ggtggaaatt ccaggggggt agcaacagct cacccatgcg gctctgacaa
     3301 cgatgttaac ccgcattaaa tatcccacgt tggacagcat ctgtctcacg gaaagtcata
     3361 ctgccttacg tgcatatttc accttacgcc ttcactgtct gtcatcggaa gtactgattt
     3421 atattggggc tttgcactta ctgtcattca cgggaaaaga tggtggtgaa cagtacgata
     3481 ctgtcttggc tgttgccgag ggaagattca ggagtggtaa gggggttagt ggtgtaagat
     3541 gagtctggag ctctgactgg tcagtgacgc gaaactcaat ctgccaggat aaactccccc
     3601 ttgcttttgc gcagaagcag tgggccttgg ttcgcttatg tcctgcatct acaagcgaat
     3661 gactgaccgg agactcccat atctggtcag tcgcccattt ttattttcca ccacatcgga
     3721 caagcatacc gtaataaaca gcaccagtct cctgttcact cttgcttgat ttactggaat
     3781 gctgtttatg gagatatccg gttgaaagat tttacctctt cgacaggact ataaattata
     3841 cagtaaggag tccctcgtag aaaggggctg attctcatca agtcggacag gttcaaagtg
     3901 ttacctgaat gagctaaatg ttatttacct tcctttctgt tcattgtaaa ttttttcttg
     3961 ctaacgcaaa tcattatcat tacgatttta gaaaagggaa atttatgaag aacaaatata
     4021 tcattgctcc gggcattgcc gtgatatgtt ctacagtcat atcatcaggt tatgccagtt
     4081 ctgataaaaa agaagatacg cttgttgtta ctgcctccgg cttcactcag caactcagaa
     4141 atgcaccggc cagtgtttca gtcattactt cagagcaact gcaaaaaaaa ccggtttcag
     4201 acctggtcga tgcggtaaaa gatgttgaag ggattagtat caccggtggg aatgaaaaac
     4261 cggatatcag tatacgtggg ttaagtggcg attatacatt gattctggtc gatggacgac
     4321 gtcagagtgg tcgggaatcc agaccaaacg gcagcggcgg ttttgaagcc ggatttatcc
     4381 ctcctgtgga agcaattgag cgtattgaag ttatacgtgg ccctatgtct tccctgtatg
     4441 gttccgatgc tatcggagga gttattaata tcataaccaa acctgttaat aaccagacat
     4501 gggatggcgt acttgggctt gggggaatta ttcaggaaca tgggaaattt ggtaactcaa
     4561 ccacgaatga cttctacctg tcaggcccat tggttaagga taaacttggt cttcagttat
     4621 atggaggaat gaactatcgc aaggaagata gtatctctca gggaacacca gcaaaagata
     4681 ataagaatat aacggcaaca ctccagttta ctccgactga aagccagaag tttgtttttg
     4741 aatacggaaa aaataaccag gaacatacat taacacctgg tgattctctt gatgcctgga
     4801 ctatgcgggg aaatcttaaa caaccaaaca gtaaaagaga aacacataac tcacgtagtc
     4861 actgggtagc agcatggaat gcccagggcg aaatactgca tcctgaaatt gctgtttatc
     4921 aggagaaagt tattcgtgag gttaaatctg gtaaaaaaga taaatataat cattgggatc
     4981 ttaattacga gtcaagaaaa ccggaaataa ccaacacgat catagatgca aaagtgacgg
     5041 catttctgcc ggaaaatgtg ctgaccatcg gaggtcaatt tcagcatgca gaactccgtg
     5101 atgactcagc cacaggtaaa aaaacgacag aaacacagtc tgtttcagtt aaacagaaag
     5161 ctgtttttat agaaaatgaa tatgctgcaa cggattctct cgccctgact ggagggctgc
     5221 gcctcgataa ccatgaaatc tatggcagtt actggaatcc aagattgtat gctgtttata
     5281 acctgaccga taatctcact ctcaaaggag ggatcgcaaa agcatttcgg gccccttcga
     5341 ttcgtgaggt cagtcctgga tttggaacac tgacgcaggg tggtgcctct attatgtatg
     5401 gaaacaggga cctgaaaccg gagaccagtg taaccgaaga gatcggtatt atttataata
     5461 atgacagtgg tttttcagca agcgcgactc tgtttaatac agattttaaa aataagctga
     5521 ccagttacga tataggcaca aaagatccag tcactgggct aaacactttt atttatgata
     5581 atgtgggtga ggcaaatatc agaggtgtgg agcttgcaac tcagattcct gtgtatgata
     5641 aatggcgtgt atctgcaaac tatacattca ctgactcccg tcgagaaagc gatgacgaaa
     5701 gtctcaatgg caagtcgctg aaaggggaac ctctggaaag aactcccaga catgcagcca
     5761 atgcaaaact ggaatgggat tacactcagg atattacatt ttattcatct ctgaattata
     5821 caggaaaaca aatctgggca gcacaaagaa atggtgccaa ggttccccgt gttcgtaatg
     5881 gattcacttc tatggatatt ggtatgaatt accagttcct gccaggcacg ctgattaatt
     5941 ttgccgtact taacgtcaca gacagaaaga gcgaagatat cgataccatt gatggtaact
     6001 ggcaggtcga tgaaggacgt cgttactggg ctaatgtaag aatatccttc tgagagaata
     6061 tagataacct gaggccgtgc gcctcaggta ccagtatagt aatcagtaac gtcagcttcc
     6121 aggttgtaat gcttttaatt ttgataaaaa taaacttaat aaattacatt tgttaaatat
     6181 tgataatttt aatttggttt tattactcca gttatactca cactctatct ttacacaatt
     6241 catcaccatt gcacctccat agcctgttga tttaaaagat ttaatttcct gtgaaacaat
     6301 caggcctgta tgaacctgat tataccacat aatcttttaa gttatttaaa tttaactctc
     6361 gggcttttct gtcagtaaaa tcagaaaaat tgcaggattt cgtttttttg ctgcgaagtg
     6421 aaatgtatct catttttact gccttcgcca gtgcgtcagg caatggcagg tagtgtacct
     6481 gcattctgct gtaacgcgta atgtcgggta gtttacttaa acttaatatg ttttatgttc
     6541 gcattattat aaatcacttt attgcctttt gtctgctttt taatggttcc cgttttgttc
     6601 acttctggct ttataaaaaa tggatgctga agaaatgaag gcagtccgcc ttacataata
     6661 ttcatgcatg atttttatgt cattccgact gagtggaatg aaaataaagg agaaaagagg
     6721 gtatatatga acctcagaaa aaaacatata tgcatagcca tatcacttgt tcttaatccg
     6781 ttccctgcat cactggcagc acaaaacact aatgtagttg aagttgatga aagcagtcag
     6841 gggtatgatg tgcatggaga taactggaag agcattgaaa tagggacaac gcaggaagtt
     6901 aaagcatccc ccgataaaaa tattaatttc gatcataatt ctcctgttgc agaaggacgt
     6961 aactctatcg cgattggtac cagtgcagag gctggtaaag aaatgacgga taaaatggca
     7021 ggggaagcca tagctattgg tgccaaggcg aaagcaacta aagaacagtc aattgcaata
     7081 ggtggcgata ccaaagctac cggatggggg gctattgtta ttggtggcga tgatttaacc
     7141 cctttgcatg gtgacagatt cggtgataaa gaaattccga ataattactc gtcatctgaa
     7201 gcatcaggag acggaagtat tgttgtgggc tcaaggagca cagctgcagg agacctgtct
     7261 gtgtccctgg gctcaataag ctctgccaac gggacggctg ctaatgcact gggagccacg
     7321 gcggaagcta acggggatat ggcgactgcg attggtttta atgcgctggc taatgccagt
     7381 aatgcgctgg ctgttggtag tcagtcctct gccggcggag aggaatccct ggctataggg
     7441 agtcagagta cgacgggcaa aaagtccacg gttgctctgg ggcagggagc tacagcctct
     7501 gccgcggagg ggaacgtggc catcggtgct gattcagtgg acaaggcagc aactccggtt
     7561 tccggcgcta ccattaagtt aaagaatggc gggactattg aatacagggg ctttgccgga
     7621 gataaggctg catcagtagt atctgttggt gatgcgggta aagagcgtca gattgttaac
     7681 gtgggggcgg gtgctgtatc tgagaaaagt acagatgcca ttaacggtag tcagttatat
     7741 gcgatagcga agacactgaa agatgacctt gatgccatta atccgacgtg gacgctaaag
     7801 gatgataagg gaggttctgc gactattaat ggtgggaagg ataataagaa tggtaacact
     7861 ttaactgtta aaggtgatgg agatattgca gttactgtta atgataaagg aatgacgatt
     7921 gacggaacaa cgctgaaaaa tgaaataaaa aatgatgcca tcaaaattaa aggtggagat
     7981 ggtaagacgc tgacgaagag tcttggtgac aatgtcgaaa tcactggcaa aggcccaata
     8041 aaaacccagg tggctggaga taaactggag atttcagcag agacagcttc gttgcctgta
     8101 aatggtggta acgggaagat tacagtaaac aatggtgatg gcgataaact tgttactgcg
     8161 aaaaacattg ctgaagccat aaaccagtct ggctggaaaa ttaaagcgga taaagatacc
     8221 ggtggtgtta tcgagggaaa tggtcaggga acagaaacac tgataaaacc cggtgatatg
     8281 gttacgctga aagccggtaa aaatctcagc gtgaaacaaa gtggtggtga atttacattc
     8341 tctgttattg atactccgtc attcagcagt gttcagctga acggtaatgg aaaaaacgga
     8401 aatgtaacgc tctcatccga aggtaataag ctgacattgt caggcggtaa tggtgccgga
     8461 caaaatggtg ttgttatcag tggtgtggca agtggactgg aaggtaagga actttcgacg
     8521 ttaacgaata ctgataaaga gatgaaaaat gccgccagtg ttgcagacct gaaaacggca
     8581 attgatggtg tgtccaccag tatgcaggga gcaggtttcg cactgaaaga ttctaaggga
     8641 caggaagtga aacagtctct tgggcagtcg attaaagtat ccggggacag caatatcacc
     8701 accacagcca tcaccaatgg ggataagggg ttaaaaatat ccctgaataa taacctgagc
     8761 gttgggagca ataatactcc cggcagcatc aaagttaacg gggctgatgg taaggaggct
     8821 gtgagtctga atggtcagga tggcacgatt aaactgacat ccgcgcctgc tgcgaacgga
     8881 aagtctgcgt cagcaaccat tgctgttaat gcaggcaaag gtggattaac agatgctgcc
     8941 ggtaccacga agacacgtct gacttatgct ccagatggaa cattaccggc agaagagctg
     9001 gcgaccatga atgacggtct ttcttttgag ggtgatgacg gaaaggttat tcataaaaaa
     9061 ctgaatgaaa aactgaccat caaaggtggt gctggtgata atgaggatgt aacagacagt
     9121 aacattcgtg ttgatgcaaa tgaaaaggga gatggtcttc tcgtccggat gaccagaaac
     9181 ctccgtgatt tggccagcgc ggtttttggt acaggtgggc agataacaac agttaatgat
     9241 aaaggcatca caatcacaac accggctgcg ggcggtggtc aggggaatac agtttccctt
     9301 accggctccg gcctgaataa cggtggaaat cagataacca gtgtggccag tggtctgaac
     9361 ggcactgcgc ttgctgacgc cggtggagat gttctgaata atgcggccaa tatcggtgac
     9421 ctgaaaaatg ctctccgtga caacagtgcg gcactggtga ataccggctt tggtctgcag
     9481 gcggatgacg gaagcaaggt gaacacgtct ctcgggaagc atatcacggt tggtggtgac
     9541 gggcagaata tcagcacgac tgttcagaac gggagcctga cggtcagact cgctgataac
     9601 attcgcctgg cagatgacgg cagtgtttct gtcggcggaa cccggatgga taaaaacgga
     9661 ctgaccatca aggatggccc gtcagtgact gtgaacggta ttgatgcggc tggccgggtt
     9721 atttccggcg tgaaagacgg ggtgaatgac caggacgccg ttaacgtttc acaactgaac
     9781 cgtgaaatca gtcaggcaac ggcagcaacg acctgggcac tgaagactga atccggtgat
     9841 gaggctgttg ctgtttcttc ccagacagtt gaagtccgcc acggacagaa tacccgggtt
     9901 tcagccatca gtaaggatga taagggaaat tacagctatg agattgatgt gacggggatt
     9961 ccggtggagt acaccgacag ccaggggaac ccgcttgtca atattggtgg tcgtttttac
    10021 acacagaccg aagacgctgc gaccgggaaa ctgacaatga cgccggctga accagcccgt
    10081 gtcagaataa gcagtgagca gcctcttgtt ctgacaaatg tggcggccgg aaatgtgtca
    10141 ccggtatcca cggatgcggt taacgggagc cagctggcat ctgcagccag aattgctggc
    10201 ggtgataatg ttgtctggaa agacggaaaa gcagcgctgg ctcctgacac cttcagtgag
    10261 ctgacaactg caggtggcgg taagacaacg gtgaatggtg gtgtacataa aacaccggat
    10321 aatgtggctg atgcagtgaa tatgctgaac cgtgaaggca cgaagtattt taaagctgcc
    10381 tcttccggtc aggcagcaaa agcggaagga cgtgacagca ttgctgtcgg acagggggcc
    10441 gtttcccggg gacaaaacag cattgccatg ggtaatggtg ctgaagtcac gcgtcaggct
    10501 gctgccggct ctgttgcagt tggcagccat gcccgtgctg gccgcgcgaa taccgggact
    10561 tatgccctga acagtcaggc tgttgcggga cgtaccggaa gtgatactgc ggtggtttct
    10621 tttggtcagc cgggacagga gcgtcagctg cagtcagtgg caccgggtgt gttgtctgcg
    10681 aattcaacgg atgccgtcaa tggcagtcag ttgcatgcaa ctaaccgtca ggttgccgga
    10741 aatacacagg cgataaacac tctcgggaat aaattcagtc agctgagcta ccgggtggaa
    10801 gagcttaacc gggatatccg tggtgttggt gcatcggctg cggccatgag cgggatacct
    10861 caggcctatc tgccgggtaa aagtctgatg gggctgggtg tcggtggata tggtggtgaa
    10921 agcgccattg caataggtgt gtcccgaatc tccgataacg gtaagatgat aatgaaactg
    10981 aatgcaggtc agaacacccg gggtaatttc tccgtcggtg ccggtgtggg ctggcagtgg
    11041 taacagggaa aagcaaggag aaatgtaatg ttatttagtc gaatgatgat gacgggtgtg
    11101 ctgtccctga tgcttgcagg atgtggaaat ctgagtcatg tcacggatga aggaacgacg
    11161 gaagaagtgg tatggccttc tccggagaag gtgatgttta atcatgacgg aacacagaaa
    11221 ggttcgtggc cactgccaga aaagttacgt cagataaagg cgggtatgaa taaggaccag
    11281 atttatcagc tggcggggag accacacttt gctgaaggga tgtctggtgt aagggaatgg
    11341 gattacctgt tcaactttac cggaccggag ggggattata cctgccagta caagatactg
    11401 tttgaccgga acatggatgc ccgctctttc tactggaagc cggaaaactg tctgccggag
    11461 gcagagacag cagaatacag ggagtcagtc cccggtgata tattatttga ttttgattca
    11521 tggactctga cagagagggg gcgtgatgca ctttcccgtg tggcagagaa gtttcaggcg
    11581 ggtcatgttg tcggggtgaa gatagaagga tttacggacc atcttggcgg gcaggcgtat
    11641 aaccggctcc tgtcagagaa acgggcggaa tcagtcaggg ggtggtttat tgcccgggga
    11701 ttcagggctg aaaggataca tgcaacgggc cgtggatatg atgcaccagt ggtgttctgc
    11761 ccggatgtgg agggcgaagc actgaaggaa tgtctgcgcc ccaaccgcag ggttgaaata
    11821 accggacaga gcgttcgtta atatgcagtg tagccccgga atgtccgggg cttttttgtg
    11881 cacagccccc agctaggctc taggctgtaa tgtcgatcag ttagtcgaac aatggcgaca
    11941 actaccttga aaaatgccgc gaatcggtga acgattcaga tgcgattatg tattgaagcg
    12001 atgtgtcggg tacttggtgt atacaggatc ggttattacg actgagtaca gcatgaccct
    12061 cagacagaaa acaaagtgat gagcggctaa aactggagat taaggtggca catatccgca
    12121 cacgcgaaac atatggaacc tagctgctcc agacggagct ggcagataac ggaattatcg
    12181 tcggtcgtga cagactggca cgtcttcgta aggagctgag gctgcactgt aagctgaaac
    12241 gcaagttcag ggcgaacacg aactctggtc acaatctgcc ggttgcgccg aatctcctga
    12301 atcagaactt tacgcctaca gcacctaatc aggtcttggt tgcagatata acgtatgttg
    12361 ccccccggaa ggatggctgt atatcgctgg cgtcaaagat gtttatacgt gcgaaattgt
    12421 cggctacgcc atgggagagc gcatgacaaa agagctgaca ggtaaagccc tgtttatggc
    12481 gctcaggaac cagcgctcac ctaccgggct aatccaccac tccgatcgtg gttcacagta
    12541 ctgcgcatac gattacaggg ccatacagga gcagtttggt ctgaaaatat caatgtcgcg
    12601 taaagttaac tgttacgaca atgctccgat ggaaagcttc tggggaacgc tgaaaaatga
    12661 aagtctgagc cactatcgtt ttaaaagccg ggatgaggcc atctcagtaa tacgggaata
    12721 cattgagatt ttctacaatc gtcagcgtcg tcactctcgt ctggggaata tctccccgac
    12781 agccttcagg ataaaatatt atcagatggc tgcttagaaa aagaacaaat ggttatgtcc
    12841 gctattgcct gtacccctca ctccggcgaa acaccagatg acttttcctg ccgggtaaat
    12901 agattcttct gaaaaattgg agactaatgt gaaatctctg gaatacatta attttgttaa
    12961 cgtattggtg ataaaaaact cagcattatt tattatataa agttccaaat tctatgaagc
    13021 ttacaagata ttgttttttt gcctgtgata attgcctgag gctaagtcat acataatgcc
    13081 gttggagttt gcaggataaa aaatatcaat tatgtgatat gaagtaagaa gtccatccca
    13141 ctctttatc
//
LOCUS       DAZGHF010000131        13087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGHF010000131 DAZGHF010000000
VERSION     DAZGHF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.132"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(465..1037)
                     /gene="hdeD"
                     /locus_tag="ACHK3A_003963"
     CDS             complement(465..1037)
                     /gene="hdeD"
                     /locus_tag="ACHK3A_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO8741984.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
     gene            1292..1624
                     /gene="hdeA"
                     /locus_tag="ACHK3A_003964"
     CDS             1292..1624
                     /gene="hdeA"
                     /locus_tag="ACHK3A_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO8741985.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            1740..2066
                     /gene="hdeB"
                     /locus_tag="ACHK3A_003965"
     CDS             1740..2066
                     /gene="hdeB"
                     /locus_tag="ACHK3A_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO8741986.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2130..2777
                     /locus_tag="ACHK3A_003966"
     CDS             2130..2777
                     /locus_tag="ACHK3A_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO8741987.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(2829..3599)
                     /locus_tag="ACHK3A_003967"
     CDS             complement(2829..3599)
                     /locus_tag="ACHK3A_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO8741988.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRIVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(3596..4588)
                     /gene="shuU"
                     /locus_tag="ACHK3A_003968"
                     /gene_synonym="chuU"
     CDS             complement(3596..4588)
                     /gene="shuU"
                     /locus_tag="ACHK3A_003968"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO8741989.1"
                     /translation="MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(4637..5260)
                     /gene="chuY"
                     /locus_tag="ACHK3A_003969"
     CDS             complement(4637..5260)
                     /gene="chuY"
                     /locus_tag="ACHK3A_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO8741990.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGITRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFINRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(5260..5754)
                     /gene="hutX"
                     /locus_tag="ACHK3A_003970"
     CDS             complement(5260..5754)
                     /gene="hutX"
                     /locus_tag="ACHK3A_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO8741991.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(5767..7104)
                     /gene="hutW"
                     /locus_tag="ACHK3A_003971"
     CDS             complement(5767..7104)
                     /gene="hutW"
                     /locus_tag="ACHK3A_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO8741992.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLTQT
                     WQEMLNQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNDDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(7124..8038)
                     /gene="shuT"
                     /locus_tag="ACHK3A_003972"
     CDS             complement(7124..8038)
                     /gene="shuT"
                     /locus_tag="ACHK3A_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO8741993.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTATERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            8722..10704
                     /gene="chuA"
                     /locus_tag="ACHK3A_003973"
                     /gene_synonym="shuA"
     CDS             8722..10704
                     /gene="chuA"
                     /locus_tag="ACHK3A_003973"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO8741994.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            10753..11781
                     /gene="chuS"
                     /locus_tag="ACHK3A_003974"
     CDS             10753..11781
                     /gene="chuS"
                     /locus_tag="ACHK3A_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO8741995.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     MPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEVVTA"
     gene            complement(11842..12372)
                     /locus_tag="ACHK3A_003975"
     CDS             complement(11842..12372)
                     /locus_tag="ACHK3A_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO8741996.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12525..13085)
                     /gene="slp"
                     /locus_tag="ACHK3A_003976"
     CDS             complement(12525..13085)
                     /gene="slp"
                     /locus_tag="ACHK3A_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO8741997.1"
                     /translation="MTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQPG
                     LYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFLDP
                     VNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYGYG
                     AFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3494 a         3058 c         3191 g         3344 t
ORIGIN      
        1 ctccttgttt cgacttaggc tggcaattgg attgccagct ttcttttaac gcattgaaat
       61 tacctgatta aataactcat tcatgtcttc aggtttttat atagcgtctg gagacacgga
      121 tatttatgca atgtaatcaa cgccagttgc aaaaaaatga acccggatta cttatttaac
      181 cgcccatagc atcaacgctt acggaactct ttgttcactt tcccacagca cttttaaaac
      241 attaaacctg accacggaaa atatcagcca tgataatgtt tgaaatggct aatttgccat
      301 ggaacgaaaa aaatcagatt aaattcagta ggttgaaata atcaacagca ggcaattgtt
      361 tcaatgatag tgcgtaattg aactgaaaca ctgtatagct aagagagtat taactggatc
      421 atagtcactt aatgtgacta tgatccaggt ggaaataggg ataattattg ctttttaacg
      481 aacaaactgg cgaagctgaa cagactggcg gcgctaaata tcagttcaat tcccaccagt
      541 gtggaaacca gcgttaccga caccatcggc gtagcaccaa ggaatatcca ggcaatgacg
      601 atatccagca caccaataac gagctgtagc caactgcctt tcattgaacg ctgacgatac
      661 caactcatca ggcgaataac ccctgccaca cagaacaaac cggcaataaa tgccgcaatg
      721 gcaaaaatgc ccagctctgg tgcgcggatg aagaaatagc cgatcaataa ataggcgact
      781 gcgacgagga aaccggataa taccggccag aaattatgac tgcggttgct gaatagcccg
      841 acaataagcg caatgcccga gcagattaat aatgcaccca ctactgtgct taaaatgtcg
      901 ccagagacga acggaaaact gatacacagc aacccgacga taaacagcag cacggcaata
      961 aactggattg ctctgcgatg ttttttaagc atctccagat caaacttcaa aattgttgcc
     1021 ttatctatat ataacataga accaccctat aaaattaaga agaaaatccc ctgctatcaa
     1081 tctatgccaa aaacgcgtgt aagaatgcag tcgatttaat aaaaatttcc taattacagt
     1141 atctgatgcg tctgtaactc attgtattga aataaaaata tctgattttg atattttcca
     1201 tcaacatgac atatacagaa aaccaggtta taacctcagt gtcgaaattg attcgtgacg
     1261 gctctttcac tttatagttg aggatattac gatgaaaaaa gtattaggcg ttattcttgg
     1321 tggtctgctt cttctgccag tagtgagcaa tgcagcggat gcacaaaaag cagctgataa
     1381 caaaaaaccg gtcaactcct ggacctgtga agatttcctg gctgtggacg aatccttcca
     1441 gccaactgca gttggttttg ctgaagcact gaacaacaaa gataaaccag aagatgcggt
     1501 tttagatgtt cagggtattg caaccgtaac cccagctatc gttcaggctt gtactcagga
     1561 taaaaaagcc agctttaaag ataaagttaa aggcgaatgg gacaaaatta agaaagatat
     1621 gtaattccgg gaatgcgtta catcgtattt ccttgcatat tgaacaggcc ggaatatctt
     1681 ctttaaaagc agctattcct cctgtttgta tataatctct atattgaatg ggttacaaaa
     1741 tgaacatttc atctctccgt aaagcgttta tttttatggg cgctgtagcg gctttgtcac
     1801 tggtgaacgc acaatctgcg ctggcagcca atgaatccgc taaagatatg acctgccagg
     1861 aatttattga tctgaatcca aaagcaatga caccagttgc gtggtggatg ctgcatgaag
     1921 aaacagtata taaaggtggc gataccgtta ctttaaatga aaccgatctc actcaaattc
     1981 ctaaagtgat cgaatactgc aagaaaaacc cgcagaaaaa tttgtatacc ttcaaaaatc
     2041 aagcatctaa tgacttgccg aattaatgag gtacaagtaa aaaggagtag caagttgagc
     2101 catcttgctg ctcctttttg catttttata tgacagcaga atttattata cgacttatac
     2161 ttgcggtaat tgcctgtggc gctattggca tggaaaggca aatgcgcggc aaaggagcag
     2221 ggttacgcac acatgtatta attggcatgg gaagcgccct gtttatgatt gtttcgaaat
     2281 atggttttgc tgacgtgctg tctttaggtc acgtcggact ggaccccagc cgtattgctg
     2341 ctcaggttgt gacgggcgtc gggtttatcg gtgcaggtaa cattctcgtt cgtaaccaaa
     2401 atattgtagg tctgacgacg gcagcggata tctgggtgac cgccgccatt ggtatggtta
     2461 ttggcagcgg tatgtacgaa ctgggtattt atggttcagt gatgaccttg ctggtgctgg
     2521 aagtcttcca tcaattaacc ttccgcctga tgaacaaaaa ttaccattta cagctgacat
     2581 tagtgaacgg taatacggta tcaatgctcg actggtttaa gcagcaaaaa ataaaaacgg
     2641 atttagtttc gttgcaggaa aatgaagatc atgaagtggt cgccattgat atccagcttc
     2701 atgccacgac gtcgatagaa gatctgttgc gattattaaa aggtatggca ggggtgaaag
     2761 gcgtttcaat tagctaatct gtatagttat ttattcgcag gagtaccagt gcggtactcc
     2821 tgttggtttt aatgatcgag gacgatcagg ggtgaatggt tggcaggatc tttaagtacg
     2881 gtaatatccg cgccgtaaag catggtcagc gcctgctgtg tcaatacgtc ctgtggttta
     2941 ccatttgcga taacctttcc tttttgcatt aatacaatac ggtctgcata acgcgcggca
     3001 agatttagat cgtgcaatac gcagcaaaca ttgaactgcc gttcgtgaac taactggcgt
     3061 aataaacgga acaagtgctg ctgatggtga atatccagcg ccgaagttgg ttcatcaaga
     3121 aataaccatt tcggtgaggg agttggttcc cagagttgca ccagcaggcg cgctaattgt
     3181 acgcgctgtt gttcaccacc ggataattgc cgatagtcgc ggtttgctaa cgcctgacag
     3241 tcacataacg ccattatttg tgctgtttca ttatcctgat taccagtgcg atgcggatgc
     3301 cgccccatct ggataacttc ttgtacgctg aatggaaacg ccatgtggct attttgacgc
     3361 atcacggccc ggtgtttcgc cagttcagta atcgaccatt catttaatgg cttattaaac
     3421 aaccgacatt cgccgctatc aggttgaaga tagccggtta actggcgaag cagcgtagat
     3481 tttcccgcac cattagggcc gagaatggca acaatttccc caccgggaaa agtcagcgag
     3541 acattatcag tcagacgccg cccctgaagg ctataaacca gattctgggc gctgatcatg
     3601 tgatcctccg gttgcgcaga atcaaccaca taaaataagg accaccaagc aggctggtta
     3661 ataatcccac gggcatttct gctggctgta ccagcgtgcg ggcaagcgtg tctgccatca
     3721 gcaataaaca ggctccggcg agggcggagc aagggattag ccagcggtga tttgctccgg
     3781 tagtcatgcg aatcagatgc gggatcacca ggccaataaa gccgataatg ccgcttaccg
     3841 atacggcggc accaacgagc agtgagctca ctaacagtaa ttgctggcgt ttacgcttaa
     3901 cgttcacgcc gaggtaatgg gcttcttcat caccgagctg cagtaaattc agcgttccgg
     3961 cgagacaagt tgttgcgata atggccggta ggatgaatga actggcaacc aataacgtcg
     4021 gccattgcgc ctgtccaaga ttgcccatca tccacaatgt gagttgtcgt agctgctgct
     4081 catcgccgat atagctcagg atgccgatcg ctgcgccgca cagggcattg atggcgatac
     4141 cggcgagtaa taattgcatc atgctgccat cgcgatggcg ctgcgtgatg agaaaaatga
     4201 tggtgcagac cactaaactt ccggcaatgg caaacaccat ttgctcatag agcaccagca
     4261 ccactgggaa ggagaagggc agcacaatag caacgccaac cattaatgca gaaccgctac
     4321 tgacgccaag taatccaggg tcagccattg ggttgcgaaa aagcccctgc ataattaccc
     4381 cggcggttgc caacgctgca cctaccagca ccgccagcaa aacccgtggt agacgaatat
     4441 tgagccagat atcgcggtat tcctcatcaa aaagggcatc aaaactgatc tttaacgctc
     4501 cctgactggc accaaacagc accagagcta gcaataatag tgatagcccc ataaagaccc
     4561 tggcggaaga gagtgaatcc ttgagcacgc aaaactccgt tacgacatta cggcgcggac
     4621 atgccgcgcc gggggatcac gccggtttca ggtccggttc aatcagactg tagacctgct
     4681 ggttaagtgc tggcgcattt gccagttcat ggatatgtgc ggcgacatca gcacggttaa
     4741 taaaaccatg acactcctga ttttgtatcc gttgtgcttt gcccgttgcc gcaccatcaa
     4801 gcagaccgcc agggcggaga atggcataat caagctggct ggtttgcaac cagctttccg
     4861 ccagcgtttt ttcccgtacc gcctgaccaa acgcagcctt tgcccgctct gataaaaacg
     4921 gccagctatc gccgcatccc agtgacgtca ccagaatcat gcgtgtgatg cctgctttct
     4981 cggcctcgtc aatcactgtt ctgtgcgcca gataatcctg agctccgccc attgttgaga
     5041 tgataagcgc atccgggcca gctgcgcggc aagcagccgc gactacgctg gcatcacagg
     5101 cgtcgcctgt aaaaacctgt acgccttgtt gcgccagttt cgtggcggca tcggcatgac
     5161 gaatgacagc aacgaccgga cgttgttccg ccagcgccag ttcaagcgtt ctggcaccca
     5221 caccttttcc gcctgcgcca aagagtagcc acggtgtcat caggcgtgtt cctttaacga
     5281 tgctgccaga gcatggaagg cactgacttg ttcgctcagg agctgacgat gatcatccct
     5341 accgaggaag attttcagca ttgcgctgcc ttccttattg aaaaagagaa tagaggcggt
     5401 atccatgccc ataaatttac gttcgatcaa ggcaatatgc gtgcagtttt ctgctttgat
     5461 atgcccggac atgccgtgct ttccgcgtag gttgaaatag ccgtggcgat ggaaacccga
     5521 aggcagttcg ccgctaaatt cgagtattac atcggtagta tgtactaacg tggtgacgtt
     5581 gccccattcg cagacggtat cccagacagt atcgaattta tcacctgaca caacagtcgg
     5641 agagggcaga tttttcacta cctccagcag cgtggtgttg tattgttcgg ctatcgcctc
     5701 cagcgtaccg tctggctccg ttttcaaaaa ttcctgtaac gagacatggc tcataaatca
     5761 ctcctgttat ggtttttcaa gcgcaattgc tggcgcatta agtacctgaa tcagttcatt
     5821 aagagactgc aaaatgttgc ttgcccagaa acgaccttca ttggtcaacc gcagacacgt
     5881 tgacgcatcg cggcttaagc ctttttggtg ccactgggcc agtaacggcg cgagtttttc
     5941 ggcatgtggc gtaagttcgt ccagcggtac acgcgccgtt tcaatgcccg actgcaaggt
     6001 atgacgccac tgcgcgttac gttcggcgtt acgcatgatc atcatcagcg gttttttacc
     6061 tgctgcgacg gattcatgcc aggtctgaag attgcgttcg ttcatccagg agtaaccatt
     6121 aatcgaaccg ccggctccgg aaccaaaggc cagacaatcg gcaccttgtt taatcagcag
     6181 gttatagaga ttgcgttcgc gtgtggtacg gccccagtgg ctgttactga tgcagcgcca
     6241 accggcatcg tccataaaat cacacccttg caggtaaaga tcgcgacgtt ctgccggaga
     6301 gggcacggta gtacgcccat tttccacggc tttgcccagc ggtgtattgg gcaggacatt
     6361 gagcgcatag agatcgacgc cgtcgagacc gatatcgcgg gcaatagcca gatcttctcc
     6421 ccaggtttgc gcatcctgac ccggcagacc aaacagcagg tcacagacca ccgcggcacg
     6481 gtcgcgtttg accaggcttt ccataaacgc aatagcggtt gggccatccg aggtgcgggc
     6541 cattttcttg cggattttgc tgttaaacga ctgaatacca attgagaagc ggtttgctcc
     6601 agcatcaagg caagcatcga ttcgctcagc gtcaaaattc agtacccggc cttcaatagt
     6661 gatttcacaa tcaggggcca gtggcagctt ttctcgtaat gtggtgatga ttctggccaa
     6721 atcgtgtgct gaaagcgcgg aaggcgtacc gccaccgaaa tagaccgcgt ggatgggggc
     6781 agactgatgc aatacgctgt ctgcttccat ttctatttca cgaatcaggg cgtcggtata
     6841 atgagcacac gcatcgtcat taaaacggtt ctgataaaaa ccacaaaacg tgcagtgtgt
     6901 cgcacaaaaa ggaatgtgta gataaaccag tcgcttacgt ggtgataccg tttgattgag
     6961 catctcttgc caggtctgtg tcagttgctc tttggcaact ggaatggccc cacgaaacgg
     7021 catcatggcg cgcctgtctt taaatggctg atcaccgtct aaagcaaaat gtggcgtgag
     7081 atccagggtg ttatttgtgt tcattcgtct gatttaaatc catttattgc ggccaaagct
     7141 gctggtgtaa agactcaacg acatcagcaa tacgcggacc cattcccaga atcaggtttt
     7201 gatcaacggt gataatgcgc tggtttttcc atgcggcagt gtgggtaatt ccggcaatag
     7261 aacgtagccg attaatatcg ccatcgacca tttgtgaggt gacgacaatc acttcagggt
     7321 tagccgcaat cagcgattcc gcgctgtagc ttttgtactg ctggtgggta gcgacgtttt
     7381 ctgctccggc aagcgaaaga atggcgtccg cgacgctgcc tttaccggca acctgtggcg
     7441 cacttccgcc tgcggagaga ataaacatgg ctttaaccgg ggcttttttg gccgccacgt
     7501 tttgctgtac tcgctccagg cgttggttga tctgtgtcac cagcgcctcg ccttgttcag
     7561 gaacctgtaa cgtttttgcc agttggcgaa tgttggcgta catctgctca agggtggctg
     7621 gtacacgcgg cagagtgacg acgttgactt tttgcgcccg cagttggtcg agcacaattt
     7681 gcggtcctgc atcctgccag gtaattacgc tatccgggcg aagtgacaaa atgccttcgc
     7741 tgctgagttg tttccagtaa ccaatatgcg gcagtttggc ggtttctggt ggataagatg
     7801 tcgtttcatc gacaccaacc acgcgttcgc cagcgcccat cgcgtagatt agctccgtca
     7861 gcgatcctcc tgcgaccacg atacgttcgg tagccgttac gctaaaagcc cagctaagag
     7921 ccagtactgc cgtaaacaat ttttttcgtt ttaccgcggt cttcgcggca gaggccacgg
     7981 ctgaaataca taacgtcagt ggggaaaaaa gaaacggcct ggtgatgatc cttggcataa
     8041 tatgagaact cttatcaaat tgagaatgat catgattatc atttgagtta tatatgaaat
     8101 acaaccgtcg attcaatacg cacggcgtta caagcgtatt tatttttgat ttttatcaag
     8161 atgcttcgtc agctaacgtt cttgttaccg actaaatttc agcgcgggaa aaagctgaga
     8221 acccagagat atcgcggctt gcagagcaaa gagaagagaa aattatcact gttaacgaca
     8281 aagtttagtg atgaggtcat gggggaataa ccataaaaac aaagtggttt ccagggcgca
     8341 ggataagttt cataaccccc tgtttattgt gttaatggcg gttaaaaaaa agcttgatca
     8401 taaaaaattg atcgataata attctcatta tcacgtttgc atttaacaaa atacggccat
     8461 atggataaca aagccatatc atttacaagg ggtaacgcac agcgctcttc actcctgatg
     8521 gggcatatcg gtttgcctga caaagagggc gtgcttttgc gtatttgccg agataccagg
     8581 caatttcatg ggatataaac gcctgtaaac tcacccggcg aatattatcg ctctgaattt
     8641 taaataaatt tatttttcgc cagccacccc ggtattaacc caggcagcca gcatttttta
     8701 ttttcctcat ggagaatcgc tatgtcacgt ccgcaattta cctcgttgcg tttgagtttg
     8761 ttggctttgg ctgtttctgc caccttgcca acgtttgctt ttgctactga aaccatgacc
     8821 gttacggcaa cggggaatgc ccgtagttcc ttcgaagcgc ctatgatggt cagcgtcatc
     8881 gacacttccg ctcctgaaaa tcaaacggct acttcagcca ccgatctgct gcgtcatgtt
     8941 cctggaatta ctcttgatgg taccggacga accaacggtc aggatgtaaa tatgcgtggc
     9001 tatgatcatc gcggcgtgct ggttcttgtc gatggtgttc gccagggaac ggataccgga
     9061 cacctgaatg gcacttttct cgatccggcg ctgatcaagc gtgttgagat tgttcgcgga
     9121 ccttcagcat tactgtatgg cagtggcgcg ctgggtggag tgatctccta cgatacggtc
     9181 gatgcaaaag atttattgca ggaaggacaa agcagtggtt ttcgtgtctt tggtactggc
     9241 ggcacggggg accatagcct gggattaggc gcgagcgcgt ttgggcgaac tgaaaatctg
     9301 gatggtattg tggcctggtc cagtcgcgat cggggtgatt tacgccagag caatggtgaa
     9361 accgcgccga atgacgaatc cattaataac atgctggcga aagggacctg gcaaattgat
     9421 tcagctcagt ctctgagcgg tttagtgcgt tactacaaca acgacgcgcg tgaaccaaaa
     9481 aatccgcaga ccgttgaagc ttctgatagc agcaacccga tggtcgatcg ttcaacaatt
     9541 caacgcgatg cgcagctttc ttataaactc gccccgcagg gtaacgactg gttaaatgca
     9601 gatgcaaaaa tttactggtc ggaagtccgt attaatgcgc aaaacacggg gagttccggc
     9661 gagtatcgtg aacagataac aaaaggagca aggctggaga accgttccac tctatttgcc
     9721 gacagtttcg cttctcactt actgacatat ggcggtgagt attatcgtca ggaacaacat
     9781 ccgggtggcg cgacgacggg cttcccgcaa gcaaaaatcg attttagctc tggctggcta
     9841 caagatgaga tcaccttacg cgatctgccg attaccctgc ttggcggaac ccgctatgac
     9901 agttatcgcg gtagcagcga cggctacaaa gatgttgatg ccgacaaatg gtcatctcgc
     9961 gcggggatga ctatcaaccc gactaactgg ctgatgttat ttggctcata tgctcaggca
    10021 ttccgcgccc cgacgatggg cgaaatgtat aacgattcta agcacttctc gattggtcgc
    10081 ttctatacca actattgggt gccaaacccg aacttacgtc cggaaactaa cgaaactcag
    10141 gagtacggtt ttgggctgcg ttttgatgac ctgatgttgt ccaatgatgc tctggaattt
    10201 aaagccagct actttgatac caaagcgaag gattacatct ccacgaccgt cgatttcgcg
    10261 gcggcgacaa ctatgtcgta taacgtcccg aacgccaaaa tctggggttg ggatgtgatg
    10321 acgaaatata ccactgatct gtttagcctt gatgtggcct ataaccgtac ccgcggcaaa
    10381 gacaccgata ccggcgaata tatctccagt attaacccgg ataccgttac cagcaccctg
    10441 aatattccga tcgctcacag tggcttctct gttgggtggg ttggtacgtt tgccgatcgc
    10501 tcaacacata tcagcagcag ttacagcaaa caaccaggct atggcgtgaa tgatttctac
    10561 gtcagttatc aaggacaaca ggcgctcaaa ggtatgacca ctactttggt gttgggtaac
    10621 gctttcgaca aagagtactg gtcgccacaa ggcatcccac aggatggtcg taatggaaaa
    10681 attttcgtga gttatcaatg gtaatcatct gccccgatat ttcggggcat ttatctggaa
    10741 ggaagagaga caatgaacca ctacacacgc tggcttgagt taaaagaaca aaatcccgga
    10801 aagtacgcgc gtgacatcgc aggtttaatg aatattagcg aagcagaact ggcatttgcg
    10861 cgcgtcacgc acgatgcgtg gcggatgcgc ggcgatatcc gtgaaattct ggcggcgctc
    10921 gaaagtgttg gcgagaccaa atgtatttgt cgtaatgaat atgcagtcca tgagcaagtt
    10981 ggtgcgttca caaaccagca tttgaacgga catgccggat tgatcctcaa tccacgcgcg
    11041 ctggatttgc gtctgtttct caatcaatgg gccagtgttt ttcacattaa agaaaatacg
    11101 actcgtggcg aacgccagag tattcagttc tttgatcatc agggcgatgc attactaaaa
    11161 gtttatgcca ccgacaatac cgatatggcg gcatggagtg agcttctggc acggtttatc
    11221 gccgatgaga atatgccgct tgagttaaaa gccgttgatg cgccagttgt tcaaacgcga
    11281 gccgatgcca gtgtggtcga gcaagagtgg cgggcgatga ccgacgttca tcagtttttt
    11341 acgttgctca agcgccacaa cctgacgcgc caacaggcgt tcaatctggt ggcagatgat
    11401 ttggcctgca aagtatccaa cagtgcgttg gcgcaaattc ttgaatctgc acagcaggat
    11461 ggcaatgaaa tcatggtgtt tgttggcaac cgtggctgcg tacagatttt cactggtgtg
    11521 gtagaaaaag tggtgccaat gaaaggttgg ctgaatattt tcaacccgac gtttactctt
    11581 catctattag aagagagcat tgctgaagcc tgggttaccc gtaaaccgac cagcgatggc
    11641 tacgtaacca gtctggaatt gtttgcccat gatggtacgc agatagcgca actttatggt
    11701 caacgtacag aaggcgaaca ggagcaagcg caatggcgta agcaaattgc ttcgctgata
    11761 ccggaagtcg ttactgcata acgtataatt aacgggaggt ctggctattt attgctgggc
    11821 ctctttatat tgatcaatcg ttcacaccag ataatcaata tgctgatggc gtaccaactc
    11881 attgatccgc ttaagattga ggatcatcat gatattgtgt ttatggcagt agaacgtttt
    11941 tagcgatatt ttcagtttct ccagaatatc ttccgttgtc atttgattcg caatgcagct
    12001 cagaacctga tgctgcttgc gcgaaaggga cacatccgct tcaggcacag agcagtaact
    12061 gccattgatg agtgcgacaa agtcgatgat ggtgatagtt cgcggaacaa aggtcaccgg
    12121 tactttcccc aggcagcgtt taatattaaa gggagagaaa ataatcacat ggacaggttt
    12181 taatcgttca atctgagtga gcaaattaga atgaaataca ttattcatta atgtatcaat
    12241 aatgacgaag gcatgctgat gcaacattac gtcgatctct tcttcgttct gtatatgaac
    12301 gacattacct ttactcagaa tgtttttcat cgcagtgaag aacatcgtat ccctggtaat
    12361 tataagaaac ataaccgact cctggtgcgg acattaactt tataatcagt ttgccaaagg
    12421 taattaacag gacagggctt ataataatag ttagctggct gtgtattaat ttgatgtaat
    12481 gcatgatgca gatgctgaga aagcatctgc atctctcggt ggtgttattt gaccagctca
    12541 ggtgttactt gactcaccgc attggtgtag taaggcgcgc cccagcccgg ttccggccag
    12601 aatgcaccat agccgtaatc ccacaggtta taggtggtat taaccacttc tctcaaatgc
    12661 cacacctgga tgccctgcat attcacttcc aggaagttat acgggacttt attgataaag
    12721 ccaggttgtt caccctgaat ggtaccgagg atggtaacaa agtgattacg atagttcact
    12781 ggatcaagga agccgctttg tctggcgagc agtcggccct gatagttggc ttcaatatca
    12841 ggcttcgcat agctatccaa cggtaataca gcgatttcta acaacgtatc cgttttgcca
    12901 ttgataacgt tgataacctt cccgccaaag cgcgcttgtt gaccaacata taaccccggc
    12961 tggttatgaa cagcaacaaa acttttttga atatcaggtt ggttattgcc tttgatattt
    13021 tgcggaattg aactacatgc ggcaagcaaa aatgaaaggc tgaggatgag tgcacctttt
    13081 gtcatgt
//
LOCUS       DAZGHF010000132        13035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGHF010000132 DAZGHF010000000
VERSION     DAZGHF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.133"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(37..402)
                     /gene="yhaH"
                     /locus_tag="ACHK3A_003977"
     CDS             complement(37..402)
                     /gene="yhaH"
                     /locus_tag="ACHK3A_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO8741998.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(693..1679)
                     /gene="yqjG"
                     /locus_tag="ACHK3A_003978"
     CDS             complement(693..1679)
                     /gene="yqjG"
                     /locus_tag="ACHK3A_003978"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO8741999.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFNHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1749..2141)
                     /gene="yqjF"
                     /locus_tag="ACHK3A_003979"
     CDS             complement(1749..2141)
                     /gene="yqjF"
                     /locus_tag="ACHK3A_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO8742000.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2328..2627)
                     /gene="yqjK"
                     /locus_tag="ACHK3A_003980"
     CDS             complement(2328..2627)
                     /gene="yqjK"
                     /locus_tag="ACHK3A_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO8742001.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2617..3021)
                     /gene="yqjE"
                     /locus_tag="ACHK3A_003981"
     CDS             complement(2617..3021)
                     /gene="yqjE"
                     /locus_tag="ACHK3A_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO8742002.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3024..3329)
                     /gene="yqjD"
                     /locus_tag="ACHK3A_003982"
     CDS             complement(3024..3329)
                     /gene="yqjD"
                     /locus_tag="ACHK3A_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO8742003.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3367..3735)
                     /gene="yqjC"
                     /locus_tag="ACHK3A_003983"
     CDS             complement(3367..3735)
                     /gene="yqjC"
                     /locus_tag="ACHK3A_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO8742004.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3882..4265)
                     /gene="mzrA"
                     /locus_tag="ACHK3A_003984"
     CDS             complement(3882..4265)
                     /gene="mzrA"
                     /locus_tag="ACHK3A_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO8742005.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4269..4931)
                     /gene="yqjA"
                     /locus_tag="ACHK3A_003985"
     CDS             complement(4269..4931)
                     /gene="yqjA"
                     /locus_tag="ACHK3A_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO8742006.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4953..5036)
                     /locus_tag="ACHK3A_003986"
     CDS             complement(4953..5036)
                     /locus_tag="ACHK3A_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742007.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            complement(5276..6052)
                     /gene="exuR"
                     /locus_tag="ACHK3A_003987"
     CDS             complement(5276..6052)
                     /gene="exuR"
                     /locus_tag="ACHK3A_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO8742008.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQATDNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6182..7480)
                     /gene="exuT"
                     /locus_tag="ACHK3A_003988"
     CDS             complement(6182..7480)
                     /gene="exuT"
                     /locus_tag="ACHK3A_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO8742009.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            7963..9375
                     /gene="uxaC"
                     /locus_tag="ACHK3A_003989"
     CDS             7963..9375
                     /gene="uxaC"
                     /locus_tag="ACHK3A_003989"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO8742010.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9390..10877
                     /gene="uxaA"
                     /locus_tag="ACHK3A_003990"
     CDS             9390..10877
                     /gene="uxaA"
                     /locus_tag="ACHK3A_003990"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO8742011.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10960..11511
                     /gene="ygjV"
                     /locus_tag="ACHK3A_003991"
     CDS             10960..11511
                     /gene="ygjV"
                     /locus_tag="ACHK3A_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO8742012.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11516..12760)
                     /gene="sstT"
                     /locus_tag="ACHK3A_003992"
     CDS             complement(11516..12760)
                     /gene="sstT"
                     /locus_tag="ACHK3A_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO8742013.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLINDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         3305 a         3380 c         3337 g         3013 t
ORIGIN      
        1 gccggatgag tcagacatcc ggcgagtaga tttgttttaa ggttccagtt ttggatcagg
       61 tccaaagcgg ttttcgcctg gcgtacccgc ctggcagtta aagacgatga taataagcca
      121 gccgataaac gggattaaga acagcagtgc ccaccacgcc gagcggtcgg tgtcgtgcag
      181 gcggcggaac tgaactgccc accacggtaa aaacaccaga ataccgtaga ttgtcgtcag
      241 gatgccttcg ccaccggcac gttgccagcc taacatttta tccagcagcc ccagcacgaa
      301 cgtaaagatg atgttgacca gaataaacat ccagtactct ttgcgccgcg cacgaccccg
      361 gaaaccgaca taatttttta gtactttcag ataccagtcc atttttgcct caattttgct
      421 caatcacttg tttttaaagc agcaaattaa gaatagacgt gaatgaaatg gcagtaaata
      481 ttcgttcaag gaattaataa agtgccgtaa agcgcgctaa gactgatgac aagcgcaaaa
      541 tcgccagatg cgctacgctt atctggccta cgcggcattg taacatattg aatataaacg
      601 ttttgtaggt tggagaagac gttcacgccg catccgacat aaataacacg cacttagcta
      661 ataatcagag gcacctcgta aggtgccttt ttttaaccga agcgaacatc tcgtccatgc
      721 ggttcatcga gatcctgcca cggaccaatt gaaataatcc ccgtagggtt gatggtctta
      781 tggctgcgga agtaatgatt acggatgtga ttgaaattga cagtttcggc gatccccggc
      841 atctggtaga tatcgcgcag aaaaccgtac agattcaggt aatcgctgat gcggtgctta
      901 tcacacttga agtgggtcac atacaccgga tcaaaacgga ccagcgtggt ccacaggcga
      961 atatcggctt cggttagctg attgccggtc aggtaacgat gctggcctaa aatctgctcc
     1021 agtcgcgcca gcgattcaaa cactttcgcc accgcctcgt cgtaagcttg ctggctggtg
     1081 gcaaaaccag ctttatacac gccgttgtta acggtgtcat aaatccagcc gttaagttcg
     1141 tcaattttcg tttgcagggc tggtgggtag taatcacccg ctttcgcgcc cagcgcatca
     1201 aacgcggtat tgaacatgcg gatgatttcc gctgattcgt tgctgacgat ggtgtggttc
     1261 tttttgtccc acagtacggg aacagtaact cgtccgctgt agtgtggatc ggcgtggaga
     1321 taaagctgat acaaaaattc atgttgatag agtgtatcgc cggttgcacc cggaaaactg
     1381 tcatcaaagg tccagccgtt ttccagcatc agcgggttca ctacagaaac ggaaataaac
     1441 ggttccagtc ctttgagttt gcgcatgatc agcgtgcggt gcgcccacgg gcaggcgagt
     1501 gaaacataga gatgataacg atctttctct gcgataaaac cgcctgtgcc agtggggcct
     1561 ggcgcgccat cggcagtgag ccagttacga aatgcggaag ctgaacgttg aaatttaccg
     1621 ccggtagatt tggtatcgta ccaggtgtca tgccagacgc cgtcaatcag ttgacccata
     1681 tttctctcct ccaatagcaa aaacgaggag cttcctcctc gttttttatc gttaagtata
     1741 gcgcgtgctt accacttttt attcagcagg cggtcgatgc tatacgcgcc cggaccggta
     1801 attgccagca gcaggaatcc gccagaaatt gtcaggtttt tcatgaacat cagcgagttg
     1861 acgccttcag caaagttgct gtgaaataaa aatgccgtca gcagcgtaaa gcccgcagta
     1921 aacagggctg tggtgcgagt caggaaaccg aacaggattg ccagaccacc accaaactca
     1981 agcagaatca ccagcggcag cataaaaccc gggacgccca tcgcttccat atattgttgg
     2041 gtacccgcgt aaccagtaat ttttccccag cctgcggtaa taaacagaat cggcattaaa
     2101 atgcgcgcta ccagtacacc aacatcttct aattttttca tcatactctc cactgcgccg
     2161 ctcaggctgc tgattgtttt tttgtgcaag ggcgcgctat tagcgtcgtt gctgtcgatg
     2221 gagagaatca taaacgtggc gaagcgtgat tgttagcaag gaaaactgtc aaatattttc
     2281 aaaaaatttg agggataagg ccggaatttc tccggccaga gaggaagtta accgcgaagc
     2341 tgttgttgct tgagggttgt tttcaccaga cgccaggcgc tccatacacc aaaaccgcgt
     2401 ctggcccagc ggaccagcat attaggatgg cgaatcgtcc agatagccat cacgctactg
     2461 ccaaccagcg cccaggagcg cagactcaac agcatattcc agcgacgatc gtaagcgcct
     2521 gttgcctcca gccattcacg acgactggcg gaaagatcca gccgttgttg ctggatctgg
     2581 ctaagcagtt gcgccttacg tcgttcacgt tcgactttac tgctcacggg actcctcctc
     2641 gagcagctgc cgatcgtttg ccagctcatg gcgcgtgtgg cgcagcaacg tagacttacg
     2701 cgatttacgt aacgtccaga tgccgccaat cagtgccagt agcagcaaca ccacggtggt
     2761 ggcaatcatc gcattcaggc gatattgcgg atcaaccgcc cagataatta gcaccatcag
     2821 gctcataaga ccaaatgcag cgaaaagcat cgtcaggccc agcatcagta aaagttgaaa
     2881 gagattcgct ttttcctctt ccagctccac caccgccagc cgcagacgtg tctccaccat
     2941 ttcaaccatg atggaaacaa ttcgctgccc gatgcccaga acgcttttac cgggcccttg
     3001 cgcgtgatga gtgtccgcca taattaacga cgcgacagca gaacaccgag cactacaccg
     3061 attgctgcgc caatgcccac gcccgtccac ggattttcac gcacatactc atcggcacgc
     3121 gccgccgcga cacgggtttg tttggcaatg gcatcaccgg tttcacccag gcgataacgg
     3181 ctctgtttca gtgcctgctc cgctttgcta cgaatcttac tcaactcttc tttcgacttc
     3241 tcgccagatg agctaagcac ctcttccagc gtatcggaaa gggatttcaa ctcagcacgc
     3301 agatgttccg tagtgtgttc tttcgacata gttttctcca ggtgagtaag tagtgactat
     3361 tgtgagttag tagtcgcgcg cttccagctt tttcagctct tcctgcgctt ctgccagttt
     3421 ccgttcgcgt ttggcaatct tatctgcatc gcctttttgc ttcgcctcgg ctaaatcttg
     3481 ctggcgttcc gccacctcat ctttctgctt tgcgattttc ttctgatgat cggcacgcag
     3541 ctggctatct gaacagttgg cccggacttc actcagggct ttattcagac cgtcaatacg
     3601 attctggttt tggtgttttt cggcatagct gatctcctta aggatatttt gctccttttc
     3661 ctgacacagg gtagtggcat aactaccggc actaagagca aagagagaaa cagctaaagc
     3721 gatgcggtat ttcattcttg aaccttccat tgtgattcgg acctattaca aaggaacgat
     3781 tgtccagaca acgaagcggt cgtgttaacc accgcctcat taatcataga cagttacggt
     3841 gaaatcacca aagtgagtga tctgtttcgg ataacaggaa gttatccgaa gcgatgagag
     3901 ttatcccgca accgggtcag ccactgcata gcctgactgt tattgtcctg ctgcgcaatg
     3961 atgtagccat ggggcaatgt tctgtcgagg accgcttttg cggcggcgct ctggtcgctg
     4021 gagtcaaagg taattaacag agtgtcgttt ttgggggtga tacttttgaa aggaatgccg
     4081 tgagcgtcaa ggtgatgcca gattgaaaaa ccgtctggca tcgttgtccc ttgatgaacg
     4141 gcacgaatcg ccagcgtaga ctcttgctgg cgaaccgcgg accaggccag caacagtgtg
     4201 ccgaccagaa gtaaaacagc gccggaccag gctagctggc gaagcgacat gcgaggtatt
     4261 tgcatccctt acccccgatt tccatatttc tttttccata acacgaccag agaacctgcc
     4321 aggccaaaca ccagcagcac caccgggagc agcatcaggc atgacatcag ctggtcctcg
     4381 tactttaaaa ataccggcgt tttgccgagc atgtaaccca gagttgtcag gatcaatacc
     4441 cacaacaaac cactcatcca gttgaagaac tgaaagcgcg cgttattcag ccctgataac
     4501 ccggcaatcg tcggcagcag tgttctgaca aacgcaataa agcgaccaat taacagcgcc
     4561 gataaaccgt gtttatgaaa aagatggtgt gcgcgttgat gataatgcgc gggtaaatga
     4621 gatagccagt tttgtacggt gcgggtattg cccagccatc gcccctgaat atagctgacc
     4681 cagcagccga ggctggcggc aacggtcagc agcagaatcg tttgcggata gcccatcgcg
     4741 cctttcgcaa tcaatacacc gaccaacacc agtaagctgt cgcccggtaa aaaggctgcc
     4801 ggaagcaagc cgttttcaag gaacaaaatg acaaacaaga caaaatacaa catgccaatc
     4861 atcgacggat tggccagggt ttcaaaatcc tgcgcccaca gggcttgcag caattgggtc
     4921 aaaagttcca ttcattattc ctggtgaatc ggttaacgcc acgctggttt taaatgatgc
     4981 actaccgcaa tgttattgct gtcttggtat gttctgcggg tttgatacat caacacagcc
     5041 tgaactattc cctgttaaat cgtgagataa gaaagcattc aaaagcataa attcaaattg
     5101 aatctaattg taacaaaggt caacgttgtt cgttatcaaa attacatgtc cgtggagtct
     5161 gattttgctg aatgcagggg agggaggcga gaggatttac acaggctgac actttatact
     5221 tttcgcttac cgacgagaca aacaggcgca ctgtagcgcc tgtcggacag gaatttcatt
     5281 tactgccgct ggtggctgta tcgagatgca ccaccgggtt ttcggcgaac agatagcggt
     5341 cggcattgaa ctcgaaatcg tcgctggttt cgttaaataa catgatcttg gtgttttcca
     5401 ggtgctgcca cattgccagc ttagcagcat gaggatcttt gcgaatcagc gccttgagga
     5461 tttgatcgtg gtcatcacac cagttatcga cggtacgggc atcaatgtgt tcgtgcagtt
     5521 ttttccagta cgggttatga ctacgctggg tccacatttt ttccacgata gccgccaggg
     5581 cggagttctg cgtcgccagg gctacctgaa tgtggaactg caaatcccac tcggaatcac
     5641 ggaagcattg ttcgccgcgc gcctgctcct ggatggccat cagtttcatg atgtcctgtt
     5701 tcgtcacctg agttgccgcg aactcggcaa tattactttc gatgagctgg cgagcctgga
     5761 gcaactcaaa cggaccgtaa ttggcgaatt ccatattatt gtcagtcgcc tgctgatggc
     5821 gcggctggtt ggaaaccaca tgaatacctg aacctttacg cacttcgaca tagccttcaa
     5881 cttccagcat gatgatggct tcacgaacca cggtgcggct gacgttcttt tcatcggcaa
     5941 taaagcgttc agcaggcagt ttatcaccca ccagatagac gccctgttcg atgcgctctt
     6001 tcagatcagc ggcaagttgt tgatacaaac ggcgtggttc agtgatttcc atatgcgctc
     6061 caggcatgat acggcagact atatttgtta taccactttt gcgagtttag ctctactcac
     6121 agtgatgaaa aagccgcctg agaaggcggc tttgaaggga aactaaaact tacacgaagc
     6181 cttaatgttg cggtgcggga tcgttatgtg attcctgcgc cacctcaatc gccggtttgt
     6241 tctgcaacac ggtccagata accagcgcac ctaacaggtc gaacactgcc agaactgcga
     6301 acagcgggct gaagccgatg gtgtcagcca gtgcaccgac taccagtgca aacagcgtac
     6361 ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg ttacgaccga
     6421 acacgtcaga cgagagcgta atcagtgcgc cagacagtgc ctggtgagca aaaccaccga
     6481 tacacagcag cataatcgcg acatacgggt tggtgaacag accgatcata cccgggccaa
     6541 tcatcagcac cgcacccagc gttacgacca tcttacggga aacgatcagg ttcacaccaa
     6601 accaacgctg gaacagtggt ggcaggtaac caccgaggat acaaccgagg tcagcaaaca
     6661 gcatcggcat ccaggcaaac atcgcgatct ctttcaggtt aaagccgtaa actttaaaca
     6721 tgaacagcgg gatccacgcg ttaaaagtac cccatgccgg ttctgccagg aagcgcggca
     6781 gcgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt ttcttcgctg
     6841 tgtcaacctg gtgctgggct tcctgaccat taataatgta gtcgcgttct tcatcggtca
     6901 gatgtttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc cagataaagc
     6961 tcaacgcacc ggagatgata aatgccatct gccagctgtg catcacgatt gcccatacca
     7021 ccagcggcgg cgcaatcatc gcaccaatcg aagaacctac gttaaagtaa cctaccgcga
     7081 tggaacgctc tttcgccggg aaccattcgg agctggcttt cagacccgcc ggaatcatcg
     7141 cggcttccgc ggcaccgacc gcaccacgag caacagccag gccgccccag ctacctgcca
     7201 gcgcggttgc accacagaac acggcccaca gcacagcaaa cattgcataa ccgattttcg
     7261 tacccagcac atccagtaca tagcccgcta ccggttgcat gaccgtataa gcagcagaat
     7321 aggctgcgat gatataggaa tactgttggg tggagatgtt taactcttcc atcagagttg
     7381 gcgcagctgc cgccacagtg ttacgcgtca ggtaaccaag cacggtgccg agcgtcacca
     7441 gtgcgatcat ataccaacgt aaccctttaa ttttacgcat gtaaaacctc atcgttttgt
     7501 tatatccgca ggaagcgcgg ccatcatccc gccagaggct gaagatgtta gttacgaccg
     7561 ggtaaccgaa aaaaaaccgg cacgccccga acgttgccat gaaagtttcg tgctaattcg
     7621 gcttccgtac cggttaaccc gattcatcta acaaatgaat gaatcggcaa tttgcattcc
     7681 gtcgacttat gatttgcgac ggcagaaaga taacttgtca tacaacttta aaaggtgaga
     7741 gccatcacaa atgtgcgaat atttgtaggg acattacctg acgacggcga ggccagtact
     7801 ggcgcggcct gcagcgagat ttaccacttt gagagtaatt tttttaacta cgtttattga
     7861 tctaactcac gaaaatatct tcggactctg gaaattggtg tgataacttt gtcagcatcg
     7921 caccataagc aagctagctc actcgttgag aggaagacga aaatgactcc gtttatgact
     7981 gaagatttcc tgttagatac cgaatttgcc cgccgtctgt atcacgacta cgcaaaagac
     8041 cagccgattt tcgattacca ttgccatttg ccgccgcagc agattgcgga agactatcgt
     8101 tttaaaaacc tgtatgacat ctggttgaag ggcgatcact acaaatggcg cgctatgcgt
     8161 accaacggtg tggccgagcg tctgtgcacc ggtgatgcgt ctgaccgtga aaaatttgac
     8221 gcctgggcgg cgactgttcc gcacactatt ggcaacccgt tataccactg gacgcacctc
     8281 gaactgcgtc gtccgtttgg tatcactggc aaattgcttt ctccgtcaac tgccgatgaa
     8341 atctggaacg aatgtaacga actgctggcg caggataact tctctgcgcg cggcatcatg
     8401 cagcagatga acgtgaaaat ggtcggcacc accgatgacc cgatcgattc tctggagcat
     8461 cacgcagaga tcgccaaaga cggctctttc accatcaaag tgctgccgag ctggcgtccg
     8521 gacaaagcct ttaacatcga acaggcgacc tttaacgact acatggcgaa gctgggtgaa
     8581 gtctctgata ccgacatccg ccgctttgct gatctgcaaa ctgccctgac caaacgtctg
     8641 gatcacttcg ccgctcacgg ctgtaaagtg tctgaccacg cgctggatgt ggtgatgttt
     8701 gctgaagcga acgaagcgga actggacagc attctggcgc gccgtctggc aggcgaaacc
     8761 ctgagcgagc acgaagtggc acagttcaaa actgcggtgc tggtgttcct tggtgccgaa
     8821 tacgcacgtc gcggctgggt acagcagtac cacatcggcg cgctgcgtaa taacaacctg
     8881 cgtcagttca aactgctggg gccggatgta ggctttgact ccatcaatga ccgtccgatg
     8941 gcagaagagc tgtctaagct gctgagcaag cagaacgaag aaaacctgct gccgaaaacc
     9001 attctctact gcctgaaccc gcgcgataac gaagtgctgg gcaccatgat cggtaacttc
     9061 cagggcgaag gtatgccggg caaaatgcag ttcggttccg gctggtggtt taacgatcag
     9121 aaagacggta tggaacgtca gatgacccaa ctggcgcagc tcggtctgtt gagccgcttt
     9181 gtcggtatgc tgactgacag ccgtagcttc ctgtcataca cccgtcacga atacttccgc
     9241 cgcattctgt gccagatgat cggtcgctgg gtggaagcgg gcgaagcacc ggcggacatc
     9301 aacctgctgg gcgagatggt gaaaaatatt tgctttaaca atgcgcgtga ctacttcgcc
     9361 attgaactga actaaggtct ggggttgata tgcaatacat caagatccat gcgctggata
     9421 acgtcgcggt cgctttagca gatttggctg aaggcacaga agtcagtgtc gataaccaga
     9481 ctgttacgct gcgccaggat gttgctcgtg gacataaatt tgcgttaacg gatatcgcaa
     9541 aaggggccaa cgtcatcaaa tatggcctgc cgattggtta tgcattggcg gatattgcgg
     9601 cgggggaaca cgtccacgcc cacaatacgc gcacgaatct gagcgatctg gatcagtatc
     9661 gctatcaacc tgattttcag gatctgcctg cgcaagcggc agatcgtgaa gtgcagatct
     9721 atcgtcgcgc taacggcgat gtcggggtgc gtaatgagct gtggatcctg ccaaccgtgg
     9781 gctgtgtcaa cggcatcgcg cggcagatcc aaaatcgttt cctgaaagag accaacaacg
     9841 ccgaaggtac tgacggcgtg ttcctcttca gccacaccta cggctgctca cagctgggcg
     9901 acgatcacat caatacccgc accatgctgc aaaacatggt gcgccacccg aacgcaggcg
     9961 cagtgctggt gattggtctg ggctgtgaaa acaaccaggt tgccgcattc cgtgaaacgc
    10021 tgggcgatat cgatcctgaa cgcgttcatt tcatgatctg ccaacagcag gatgatgaga
    10081 tcgaagccgg gatcgcgcat ttgcatcagc tgtataacgt gatgcgcaac gataaacgcg
    10141 agccaggcaa actcagcgaa ctgaagtttg gtctggagtg cggtggttct gacggtcttt
    10201 ctggtattac tgctaacccg atgctggggc gtttctctga ctacgtgatt gctaacggtg
    10261 gtactaccgt actgaccgaa gtgccggaga tgtttggcgc agagcagttg ctgatggacc
    10321 attgccgcga cgaagcaacg tttgaaaaac tggtcaccat ggtcaacgac ttcaagcagt
    10381 actttattgc ccatgaccag ccgatctacg aaaacccatc accggggaac aaagcaggcg
    10441 gtatcaccac gctggaagat aaatcgcttg gctgtaccca gaaagcgggt tccagtgtcg
    10501 tggttgacgt gctgcgttac ggcgagcgtc tgaaaacgcc ggggctgaac ttgttaagtg
    10561 cgccgggtaa cgatgccgta gcgaccagcg ccctggcggg tgcgggctgc catatggtgc
    10621 tgttcagtac tggtcgtggc acgccgtatg gtggatttgt gccgacggtg aaaatcgcca
    10681 ccaacagtga actggcggcg aagaaaaaac actggatcga ctttgacgcg ggtcagctga
    10741 tccacggtaa agcgatgccg cagttgctgg aagaattcat cgataccatc gttgagtttg
    10801 ccaacggtaa gcaaacctgt aacgagcgta acgacttccg cgaactggcg atcttcaaaa
    10861 gcggcgtaac gctataaagt cgaaatgagt tgttaataaa aaacggcgtt tcataatgtg
    10921 aagcgccgtt tgcttttcca gacctgctaa ggatttatca tgaccgcgta ttggctggcc
    10981 cagggcgtgg gtgtcatcgc ctttctgatt ggtatcacaa catttttcaa ccgtgacgaa
    11041 cgccgcttta aaaagcagct ttcggtctat agcgccgtta ttggcgtaca tttttttctt
    11101 ctgggcacct atcccgccgg tgccagtgcc atccttaatg ccattcgtac gctgattact
    11161 ttacgcacgc gcagcttatg ggtaatggcg atttttattg tgctgactgg cggaattggc
    11221 ctcgcgaagt tccatcatcc tgtcgaacta ctgccggtaa tcggcacgat tgtcagtacc
    11281 tgggcgctgt tccgctgtaa agggctgacc atgcgctgcg taatgtggtt ttcaacgtgt
    11341 tgctgggtga ttcacaactt ctgggcgggg tcgataggcg gcacgatgat tgaggggagt
    11401 tttctgctga tgaatggtct gaatatcatt cgtttctggc ggatgcagaa aaggggaatt
    11461 gatccgttta aagtagagaa aacccctccc gccatagacg aaaggggtta aacaattaat
    11521 tacgcagggc gctatttgcc agacgatcgt cttccgcctg gcaagctgcc gcagtgaaca
    11581 gcacgtcagt tgaagagttc agcgcggttt cgcaagagtc ctgcaatacg ccgatgataa
    11641 agccgacggc aaccacctgc atggcgatat cgttcgaaat accgaacata ttacaggcca
    11701 gtgggatcag cagcagagac ccccccgcca cgccggatgc gccacaggca cacagagaag
    11761 ccactacgct caacagcagc gctgtgggca gatcgaccgg aatacccagc gtattaaccg
    11821 cagccagcgt caacacggta atggtgattg ctgcgcccgc catattgatg gtggctccca
    11881 gcggaataga aacagaatag gtatcgcgat ccagattcag cttttcacac agcgccatat
    11941 tcaccggaat gttcgctgca gagctgcggg tgaagaaggc atacacaccg ctttcacgca
    12001 ggcacagcag caccagcggg aacgggttac gacgaatttt ccaccacacc agcaatgggt
    12061 taaccaccag cgccaccagc agcatacagc caaccagcac gaccagcagt tgcgcgtagc
    12121 cccacagcgt ggagaaaccg gtggttgcca gggtagaaga aaccagacca aaaataccga
    12181 tcggtgcgaa gcgaatcacc agtttcacca taaaggtgac ggcattcgac atatcgttga
    12241 tcaggttttt ggtggtctcg ttaccgtgac gcagtgcgaa accgaggcca attgcccaca
    12301 ccaggatccc gatatagtta ccttttagca gcgcgtcaat ggggttggaa accatgctca
    12361 ttaccagccc gcgcattact tctacaatgc ctgacggcgg cgaaatatca cccgcgctac
    12421 tggacaagtg cagggtagaa gggaaggcaa aactgaagat tactgcggcc agagcagcag
    12481 agaaggtgcc cagcagatag aggaacaaaa tagggcggat attggttttc tgcccgtgct
    12541 ggtggttagc aatagatgcc attaccagca tcaacaccag gatgggggca acggctttca
    12601 gtgcgccgac gaacaaagta cctaacagac caacagcttc cgccgcgggt tttgagatcc
    12661 atgccagaag aatccccaga acaaggccga ccaggatttg ttttaccagg ctgccatgag
    12721 ccagacgccg gaatagcccc ggtgaacgtt gcgtagtcat ttttcgatcc tttcattgtg
    12781 ttgtcgcaca tccttggtgc gttctgtcga cgcatctttc aggatgtaac aaagtgtttg
    12841 caaggtcgag tataaggaaa gttacagctt cgggaagcga aaaatgctgg attttaaaga
    12901 gaggtcatat tttgcaactt aatggttaca tttatttgac ataaacagag ggaaggactg
    12961 gctactaagg ctgattagat aacatatggg caaaactatt tggaatatga gaacggttgc
    13021 gctcctttaa tgtgc
//
LOCUS       DAZGHF010000133        12797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGHF010000133 DAZGHF010000000
VERSION     DAZGHF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.134"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..420
                     /locus_tag="ACHK3A_003993"
     CDS             <1..420
                     /locus_tag="ACHK3A_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO8742014.1"
                     /translation="GLQNAEQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLN
                     DEQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRA
                     LAAELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            435..1292
                     /gene="menB"
                     /locus_tag="ACHK3A_003994"
     CDS             435..1292
                     /gene="menB"
                     /locus_tag="ACHK3A_003994"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO8742015.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1292..2254
                     /gene="menC"
                     /locus_tag="ACHK3A_003995"
     CDS             1292..2254
                     /gene="menC"
                     /locus_tag="ACHK3A_003995"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO8742016.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSGLLAWVNNWLAGDSELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSLLPLVDVDALERLL"
     gene            2251..3606
                     /gene="menE"
                     /locus_tag="ACHK3A_003996"
     CDS             2251..3606
                     /gene="menE"
                     /locus_tag="ACHK3A_003996"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO8742017.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AMQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMVEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLDEWVKDKLARFQQPVRWLNLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3716..3982
                     /gene="pmrD"
                     /locus_tag="ACHK3A_003997"
     CDS             3716..3982
                     /gene="pmrD"
                     /locus_tag="ACHK3A_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO8742018.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(3976..4362)
                     /gene="arnF"
                     /locus_tag="ACHK3A_003998"
     CDS             complement(3976..4362)
                     /gene="arnF"
                     /locus_tag="ACHK3A_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO8742019.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4362..4697)
                     /gene="arnE"
                     /locus_tag="ACHK3A_003999"
     CDS             complement(4362..4697)
                     /gene="arnE"
                     /locus_tag="ACHK3A_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO8742020.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4694..6346)
                     /gene="arnT"
                     /locus_tag="ACHK3A_004000"
     CDS             complement(4694..6346)
                     /gene="arnT"
                     /locus_tag="ACHK3A_004000"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO8742021.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGAMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6346..7236)
                     /gene="arnD"
                     /locus_tag="ACHK3A_004001"
     CDS             complement(6346..7236)
                     /gene="arnD"
                     /locus_tag="ACHK3A_004001"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO8742022.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGMLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7233..9215)
                     /gene="arnA"
                     /locus_tag="ACHK3A_004002"
     CDS             complement(7233..9215)
                     /gene="arnA"
                     /locus_tag="ACHK3A_004002"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO8742023.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPTIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LTVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9215..10183)
                     /gene="arnC"
                     /locus_tag="ACHK3A_004003"
     CDS             complement(9215..10183)
                     /gene="arnC"
                     /locus_tag="ACHK3A_004003"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO8742024.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10187..11326)
                     /gene="arnB"
                     /locus_tag="ACHK3A_004004"
     CDS             complement(10187..11326)
                     /gene="arnB"
                     /locus_tag="ACHK3A_004004"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO8742025.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKSTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            11634..12236
                     /gene="ais"
                     /locus_tag="ACHK3A_004005"
     CDS             11634..12236
                     /gene="ais"
                     /locus_tag="ACHK3A_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO8742026.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(12275..12700)
                     /gene="nudI"
                     /locus_tag="ACHK3A_004006"
     CDS             complement(12275..12700)
                     /gene="nudI"
                     /locus_tag="ACHK3A_004006"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO8742027.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
BASE COUNT         3162 a         3267 c         3355 g         3013 t
ORIGIN      
        1 gggttgcaaa atgctgaaca acgcacggaa cgtcagcgtt ccgatcgcca atgggcgcag
       61 cgttttcgca cagaaccgtt aacggcggta tttgctgact ggtatcaaca gcctgttttt
      121 gcctcactca atgacgaaca acgccgggag ctggttgcgc tgcgcagcaa caataatggc
      181 gcaacacttg ccgccatgct ggaggcgact tctctcgccg tccagcccga tttacgtgct
      241 aaccttagcg cccgcacatt tgcgttttat tatttatgtg gtgaacgtga cagcaaattc
      301 cgcgccctgg cggcggaact ggctgccgaa tgccatgtca ttcctcgcgc cggacataac
      361 gcgcaccggg aaaatcccgc tggcgtaatc gcaagtctgg cgcagatctt gcgtttctga
      421 ctaaaggaca caatatgatt tatcctgatg aagcaatgct ttacgcaccg gttgaatggc
      481 acgactgctc cgaaggtttt gaggacattc gttatgaaaa atccaccgac ggtatcgcaa
      541 aaatcaccat taatcgtccg caggtgcgca atgccttccg tcctctgacg gtaaaagaga
      601 tgatccaggc actggcagat gcgcgttatg acgacaacat cggcgtgatc attctgactg
      661 gcgcaggcga taaagcgttc tgctccggcg gtgaccagaa agtgcgtggt gattacggcg
      721 gctataagga tgattccggc gtacatcacc tgaacgtgct ggacttccag cgtcagatcc
      781 gtacctgccc gaaaccggtt gtcgcgatgg tggctggcta ctccattggt ggcggtcacg
      841 ttctgcacat gatgtgcgac ctgactatcg cggcagataa tgccatcttc ggtcagactg
      901 gcccgaaagt cggttccttc gacggcggct ggggcgcttc ctacatggcg cgcatcgtcg
      961 ggcagaaaaa agcgcgtgaa atctggttcc tgtgccgtca gtacgacgcg aaacaggcgc
     1021 tggatatggg ccttgtaaat accgtggtgc cgctggcgga tctggaaaaa gaaaccgtgc
     1081 gctggtgccg tgaaatgctg caaaacagcc cgatggcgct gcgctgcctg aaagctgcac
     1141 tgaacgccga ctgtgacggt caggcgggtc tgcaggagct ggcgggcaac gccaccatgc
     1201 tgttctacat gacggaagaa ggtcaggaag gtcgcaacgc tttcaaccag aaacgtcagc
     1261 ctgatttcag caaatttaaa cggaatccgt aatgcgtagc gcgcaggtat accgctggca
     1321 gatccccatg gacgcggggg tggttctgcg cgacaggcgg ttaaaaaccc gcgacgggct
     1381 gtatgtttgc ctgcgtgagg gcgagcgcga agggtggggg gagatctccc cactaccggg
     1441 cttcagccag gaaacctggg aagaggcaca aagcgggctg cttgcctggg taaataactg
     1501 gctggcaggt gatagcgagc taccgcaaat gccttccgtc gcctttggcg taagctgtgc
     1561 attggcggag ctggcagaca cgctcccgca ggcggccaat tatcgtgcgg caccactgtg
     1621 taatggcgat ccggacgatc tgatcctgaa acttgccgat atgccgggcg agaaagtggc
     1681 gaaggtcaaa gtgggattgt acgaagccgt gcgcgacggc atggtggtca atctgttgct
     1741 ggaggcaatt ccggatctgc atttgcgtct tgacgcgaac cgcgcctgga cgccgctgaa
     1801 aggccagcag tttgccaaat acgttaaccc ggattatcgc gaccgcatcg cgtttctcga
     1861 agagccgtgc aaaacccgcg atgattcccg tgcgtttgcc cgtgaaaccg gcattgccat
     1921 tgcctgggat gaaagcctgc gcgagccgga ttttgccttt gtggctgaag agggcgtgcg
     1981 cgcggtagtt atcaaaccca cgctcacggg cagcctggag aaagtacgcg agcaagtgca
     2041 ggcggcgcat gcgctggggc tgacggcggt gatcagttct tccattgagt cgagcttagg
     2101 tctaacgcaa ctggcgcgga ttgctgcctg gttaacgcca gacaccattc cgggactgga
     2161 cacgctggat ctgatgcagg cgcagcaggt tcgtcgctgg ccggggagcc tgctgccgtt
     2221 agtggacgtt gatgcactgg agcggctgtt atgatcttct ctgactggcc gtggcgtcac
     2281 tggcggcaag tgcggggaga agccatcgcc ttacgtctta atgacgagca actcaactgg
     2341 cgcgagcttt gtgctcgcgt cgatgaatta gcctccagct ttgcgatgca gggtgtggtt
     2401 gagggcagcg gcgtgatgtt gcgggcgtgg aatacgccgc aaacgctgct ggcctggctg
     2461 gcgttactgc aatgcggggc gcgggtgctg cccgtgaatc cacagctgcc gcaaccgtta
     2521 cttgaggaat tgctccccaa tcttacgctg caatttgcgc tggtgccgga tggggaaaac
     2581 acgtttccgg cattaacgtc gctgcacatt cagcgggttg aaggcgcaca tgccgctacg
     2641 tggcagccga cgcgtctgtg ctcaatgacg ttgacctcgg gttctaccgg gctgccgaaa
     2701 gccgctgtcc atacttatca ggcccatctt gccagtgccg aaggggtgtt atcgttgatt
     2761 ccgtttggcg atcacgatga ctggttgctc tctttaccgc tgtttcacgt ctccggtcag
     2821 ggaattatgt ggcgctggtt atacgctggt gcgcggatga cggtgcgtga taaacagcca
     2881 ttggagcaaa tgctggcagg ctgtactcac gcttcactgg tgccaacaca actctggcgt
     2941 ttgctggtta accgcagttc cgtttccctg aaagcggtgt tacttggcgg cgcggctatc
     3001 ccggtcgagt tgacggaaca ggcgcgcgag caggggattc gttgcttttg cggctatggt
     3061 ctgaccgagt ttgcctccac ggtgtgtgcg aaagaagccg acggtctggc agacgttggt
     3121 tcgccgctgc cgggtcggga agtgaaaatc gttaatgatg aagtgtggct gcgggctgct
     3181 agtatggtag aaggttactg gcgtaacggg caactggttt cactggttaa tgacgaaggc
     3241 tggtacgcta cgcgcgatcg cggtgagatg cataatggca agctgaccat tgtcggacgt
     3301 ttagacaatc tattcttcag tggcggagag ggtattcagc cggaagaagt cgagcgcgta
     3361 attgctgcac atcctgcggt tttgcaggtg tttatcgtcc ccgttgccga caaggagttt
     3421 ggtcatcgac cggtggcggt ggtggagtat gaccagcaaa ccgttgatct tgacgaatgg
     3481 gtgaaagaca agctggctcg ttttcaacaa ccggtgcgct ggctgaatct gccgccggag
     3541 ctgaaaaacg gcggtattaa aatttcacgt caggcgctaa aagagtgggt gcaacgccag
     3601 caataattaa ccctccgttt cacttctcat ttctgccatt ccattgccgt tgataaagag
     3661 tttatctgtt aatctgtaaa ttgatgtgaa aactcttagc aaacaggata atgcaatgga
     3721 atggctggtc aaaaaatcgt gctgcaataa acaagataac agacatgtaa ttatgctctg
     3781 cgatgctggc ggcgcgataa aaatgatcgc cgaagtgaag agtgacttcg ccgtgaaagt
     3841 gggggattta ctctcgcctt tgcagaatgc gctttactgt attaatcgtg aaaagctgca
     3901 cacggtgaaa gtcctttctg caagctgtta tagccccgat gagtgggaac ggcagtgtaa
     3961 agcggcaggg aaaactcagt aacgttgttt tgttgtgggc aggaaaatca gcatcaaccc
     4021 gctcataata caggctactc ccagtagtgc tttcagcgaa aaagtccctt cccagccggg
     4081 aagtaccatt gaagcaatcc ataccagcac ataactcata ctgagcaagg cataggcttt
     4141 gcttagtgca agtttatgta gtgttttata ccaacaaaaa acggaaagca gataacccag
     4201 cagcccgagt aacagtatcc gggcatccag gccaaaagct aacaacgccg cgataaaatc
     4261 ccataagtgc gtcatcggcg gcagatgact cgccgcaaaa cccaggctta attgcgcagc
     4321 ggaggcgata atgacgctga ataatcccca catcaggccc attacacggt actcccgagg
     4381 atcacaatgc caccaataat gaacgccacc ccacaccagt gacgcggcga taccggttcg
     4441 tgccacagtt ttactgcagc cagcgtcacc cagacaaaat tcagacttaa catcgggtag
     4501 gcaatgccta ccggtacgtt ctgcaagacc agcagccaga gcaccatggc aagaccaaga
     4561 caagccagcg ccagtcccag ccacagcacg atatgtttgc gccgtttgtt tatcgccaca
     4621 aagcaggttg cctgtttctg acacaactgc ccggcaacgc taagcaagct ggcaaagact
     4681 aatgttagcc agatcatttg ggacgatact gaatcagcac cagacgctcc tgacgatcga
     4741 tggcatctgc gggcggaatg gcgagactgt tgatatcttc attgcggtca accgaaagca
     4801 cgagtgtaat aatcccctcc tgacgatgtt gattaagcca gtttgcgaac tcatcaccgc
     4861 tgacaaatct ccctttcgca tccggataat taaggccgta tttcaactca cctgtctggc
     4921 gatacatgat gatgtcatcg cgttgcaggc tccatgccag acctgcggca acaccgacgc
     4981 tatcagtaag aatatagcgg cttggctgaa gtgattcttg tgtcatctcg acaaaaaatt
     5041 gcggatgttt tccttccatg actctgtcag gaattgaaaa tcctaccagc aacgccagcc
     5101 ccagcgggca aagcgcggca aaagaccagg tcttttcgac gtttgttaag gtgtaccagc
     5161 cgaaaaatgc ccatagcgaa aaaatcgacc aggcacaaaa gactttatag ctctcgaagg
     5221 tttgccacac cggcgtgttc attgctcccc agggggagac cacaaatgtg gcaataatgc
     5281 cagtgacgcc aaaagcgatg ttaatccagc cattaatccg caacgccaga ggattatttt
     5341 ttgctgccag caaagcgtaa tgcgccatca gcattgccag aggagcaaag caagaaagaa
     5401 tataggtggg caatttgcct ttggcgacgg agaaaaacaa cagcggcatt atcgtccagc
     5461 tcaacaaata gacggttgcg gaatgcttgc ggtttttcca gcctgcgtac agtgctccgg
     5521 ggagtaatcc cagccacggc aggctaccgg caatgatgac cggcaagtag taccagaacg
     5581 gagctctatg ttgggcatca tccattgcaa agcgttgaat atgctcaacc cagaaaaaat
     5641 agtgccagaa gtcaggctcc cgctgcgcta tcgccagtcc ccaggggaga accgtcagta
     5701 cgcaactgat gaccgccagc cagccgtaaa taaagagatc tttccagcgt ttttgcgttg
     5761 caacccatgg aagcacgctt aataccggca cggcaagggc gagaaaacct ttggtcatca
     5821 cccccatgcc gcaagtgatt cccagcagta aaaatcccgc gcttttgcct ttccacgtct
     5881 gtgcctgcat tgccagccag aagctgcaca ttcccgccac cagccagaag gcaataaacg
     5941 gatcgagcac ggcataagtg ccaatggcat agacaataaa caatgagaga taaatcactg
     6001 cggcgagtag agccagacgt ttatcgcgcc ataagcgcag agtaaaccag gtcaccagcg
     6061 cggcagttaa cagggtcgca aagataacgc ctgcccgcac accaaagtta ttcgcgccaa
     6121 atagccattg cccaatgctg ttaatccagt atccggcaat gggtttttcg aaataacgta
     6181 gccctaacag atggggcaca atccagtcgc cggatgccag catttctcga ctgatttccg
     6241 cataacgcgt ttcatcgggt tgccatagca gacgcgtgct gatcggtaac aggtaatagc
     6301 aggcaataaa cgcgaagagg ccgataaggt aacgtaccga tttcatcagc gactacccgc
     6361 aatttgttgg caacccagcc agccttcacg tccggcaata tttccgcgaa caacttgtcc
     6421 gagcggcaac atccctgata acagttcgct taaagggcaa aatgtcacgc cttcctgggc
     6481 tgcccgttta aggagatcca caaaattatg ctgataagcg cagccttcaa cttctgcatg
     6541 aatggtataa accggcgtgc ctttatctcg caggatgcga ttgagtaacc aaccgttaaa
     6601 atcttctgct ttcacatccc gaccaatcac ttcatcccag gtgggtaagg tcaccggaat
     6661 ttgcgcagta ccagggtttc ctgattcgag cagtggacga aacggcatcg ccccacgaca
     6721 atcgctgttg tagcgcaaat ggaacgcttc ttttgcttcg atcacctgct ggtcggcacg
     6781 ccagccagcg gctgcggagc aggttaccgg ttggccgata atttcttcca gggtgcgaag
     6841 gccgcgtgcg atatcgtcga tcattgtttg ccgatcccaa ttaccgctat gggcttgcca
     6901 ggcatggtga tcccaggcgt gcaggccgac ttcgtgatgt ttagccgctt cacgaatgat
     6961 atcggcattg gcatgaccaa tctctttacc cggccaggcc gtacctgcca gtaaaatatc
     7021 ccagccataa agcgatgccg cgtttgagcg cagcatcttc cacaaaaact gtggcttcac
     7081 cagtcgccag agatggcggc ccatattgtc cgggccgacg ctgaaaaaaa tgctggcctg
     7141 aatattatgc ttactcaaga tttccagcag acgcggcacg ccttcacggg tgccacgaaa
     7201 ggtatcgaca tcaatgcgta agcctacttt ggtcatgatg gtttatccgt aagatcaacg
     7261 gtgcgcagga agaaatccag cgtttcgtcg atggtttcct gcatatcaat tttcggttcc
     7321 cagtccaggc agcggcgggc attgcggatg ctcggtttac gatgctctac gtcctgatat
     7381 cctttgccat agtagctgct actttcgaca acacgaaagc ctgcaaacgg cgggaagtga
     7441 tggcgcagcg gatgtttttc gaagctcgcc agcagcatct cgcccagttc ctcaatgctc
     7501 gcttcgttct caggattgcc aatgttgata atttcgccgt cgcagcgatt tcccgcattt
     7561 tcgataatgc ggtacaacgc ctcgatacca tcccggatat cagtaaagca gcgtttttgt
     7621 tttccgccat caattagctt aattggtgaa ccttctacca gattgagaat gagttgcgta
     7681 atagcgcggg agctgccaat tcgcgctgca ttaaggttat ccagtcgtgg tcccatccag
     7741 ttaaacggcc ggaagagggt gaactgtaaa ccctcttttt cgccataggc ccagatcacc
     7801 cgatcaagta attgttttga caccgaataa atccagcgtg gtttattcac cgggccgacg
     7861 gttaaattag aatggtcctc atcgaagtat ttatcgctac acatcccata aacttctgaa
     7921 gttgacggga agatgattcg cttacggtac ttcacgcagt agcggataat gcgcagattc
     7981 tcttcaaaat cgagttcaaa tacgcgcagc gggttgcggg tatattcaat cggcgtggct
     8041 atcgccacca gcggcaagac gacatcacat tttttgacgt gatactcaat ccattcggaa
     8101 tgaatactga tatcgccttc gacaaagtga aaatgcggat gattcagaaa acggcttatc
     8161 gcatcgctgc caatatccag accgtaaact tcataatgat cttcgcgcag caggcgttct
     8221 gtcagatggt tgccaataaa gccattcacc ccgaggatga gtacccgggt acggcgtcgg
     8281 gcggcacagg caggctggct attcaagcgt gaaccttgca ccaggcccag cgtctgcgct
     8341 aattgcgagc cctgcatagt aatgccgtcg cccgcctgtc cggtgacgat ttccagcgcg
     8401 ccatcgccac aggcaatcag cagtggcgct acagaaatca cgctccccgg ctgtgctttg
     8461 ctggcatgag gatgaacacg cgacgaccag acggtgaatt tctgattgcc aacatagctg
     8521 aaggcacccg gccacggatc ggcaacggca cgtaccatgt tgtgcagtac ggatgccggc
     8581 ttatgccatt cgaggaaact gtcatccggc gttctgcgac caaaacaggt agcttcgttt
     8641 tcgcgctggg cgatttccag aatattaccg tgtttaatgg tgggtaatgt ctgctccagt
     8701 agctggcgcg cggcatgaca taacttatga tgcaacgtaa tggcgatatc gtctggcgca
     8761 atggcaatgc gcagttgcgc cacaatggcc ccggcatcgg cacgtttcac cattcggtgc
     8821 aatgtaacgc cagtttccgt ttcaccgttg accagcaccc agttcagcgg cgcgcgacca
     8881 cgatattttg gtaacagcga accatgcaga ttaaatgcac ctgcgggagc gagctgcaaa
     8941 atctcgtcgc aaataagatg gcgataataa aaagagaaaa tcacctctgg tgagagttgg
     9001 gcaatacgtt ccacccacag cggatgatta acgttatctg gcgcataaac cggaatgcct
     9061 ctttccgccg ccagacgagc taccgaacca taaaaggctt tttcaccggg attatcggta
     9121 tgagtaaaaa tagcgctaat ttcgtaaccg gcagccagca gggcttcaat accgaggcat
     9181 cccatatcgt ggtaggcaaa aacgacggtt ttcattattc attttccttg ctggatggac
     9241 ggataacttg ctgaacaaaa tagcgggggc gggcgcggac atcggtgtag atcctgccga
     9301 tatattcacc gagtaatccc atgccgataa actgagcgcc aataaaagta aacagcacgg
     9361 caaatagcat aaagacgcct tctgccgccc attgtggtcc gaaggtcagg cgtaaaatca
     9421 ccagcagcac cgcaatgcta aaacctccaa tcgcaataat gctgccgagc agactcagca
     9481 tacgtagcgg cgttgtggta aggcaggtca ccaggtcgta catcaaatta atcaggcgca
     9541 taaaactgta tttggattca ccaaactcac gctcggcatg gtgcactgga atttcaatgg
     9601 cacggcgggc gaagatattc gccagaatcg ggataaaggt actgcgttca tggcagtgca
     9661 acatcgcatc gacaatatga cggcgatagg cgcgcagcat acaaccgtag tcacccatcg
     9721 ctttgccggt ggtgcgctga ataagccggt taatcatctt cgaagcggtt ttacgaaacc
     9781 agctgtcctg gcggttctgg cgtacagtcc ctaccacgtc gtaaccttca tcggcttttg
     9841 ccaccagacg ggggatttcc tctggcggat tctggagatc ggcatcgagg gtaataatta
     9901 agtcgccagt aacgtgactg aatcccgcca taatcgctga atgttgcccg taattgcggt
     9961 taagcaaaat agacacaata tggctgttct ccgcttgtga ggcttcgacc agcatatgcg
    10021 cggaattatc gctactgccg tcatcaatca gcaggatttc atactctttc cccagtgatt
    10081 cacaggctgc ggtggtgcgc ctgattaatt ccggtaagct ttcctgctcg ttataaacgg
    10141 gaataaccac cgagactttc ttaacagggt ggatttcaaa catggcttat tgtcctgcga
    10201 gttgctgaag ggctgtgatg acgcggtcgg catcggcggt ggtcatatcc gggaacagcg
    10261 gcaacgaaca gatgcgttcg ctattccatt cggtattcgg taacgacagc gtgggaaaac
    10321 gctcgcgata atatttttgt gtgtgagcgg cgcggaaatg taacccggta ccaatgcctc
    10381 tttcttttaa cgcttccatc aacgcatcgc gactgatacc acaacgttgt tcatcgacac
    10441 gaataataaa cagatgccag gcgtgaacgt gtgaccaggc gggaaggctt aatggctgaa
    10501 aggggagagc tgccagtgct tgctgatatt gctgggcaat ttcgcgccga cgggtgttga
    10561 ggtgctctaa tttgactaac tgtgtcaggg caatcgcggc gttaatatcg gtcagattgt
    10621 acttatagcc cggcgttaag acttcagcct gcggtgcacg gccccaggta tgtctgtcat
    10681 aggcatcgac gcccagaccg tgaaatttta gcatccgtag ctggcgggca aggttttcat
    10741 tatctgtaac aatcaggcca ccttccgcac aggtaatatt tttgatggcg tgaaacgaaa
    10801 aaatagcggt actttttgcg ccaatatgtc gccctttgta atacgtacct acggcatggg
    10861 cagcatcttc gataactgcg atgccgtaac gttcgccaat ggcgcgaatg gcgtcaatat
    10921 ctgctggcgc accggcataa tgcaccggaa tgatggcttt agtgcgtggc gtaatggctg
    10981 actcgatcgc ttcaggcgtg accatcagcg tatcgcggtc gacatccacc attaccggcg
    11041 ttgcacccaa caaggagatc atattgaggg ttgaaaccca ggtcagggaa ggcgtaatca
    11101 cttcatcgcc cttgccaatt tccaacgcca ttagcgtgat atgcattccg gcggtggctg
    11161 aactgaccgc gatggcatgc tgatttcccg tcaactggca aaaggcttgt tcaagcgcct
    11221 gattcttcgg accggttgtg atccaaccgg attcgagaac ctctttaact gctgcgagtt
    11281 cctccacgcc cattgctggt cgcgaaaaag gcaaaaattc tgacattgct tttccttccg
    11341 ccattgaata cctgtccact tatatttgct ataaagagtg ttgtgtatat tttgccattt
    11401 ggagcgaaat tctaaggata gaatattaac ttaaccttaa gaaactaata ttagacgtaa
    11461 atattgaaat ttttatattt tttcttattt aggctttgca tttggcaaaa ttttgaggca
    11521 ttttgccggc atcgtaggat ttttaatatt acaccaactg ctaattatcg ccagaaatgt
    11581 cgctcaattt cacggtaatt gtctggttgc gcttgtctat aggtggagtt tacgtgttag
    11641 ctttttgccg ctcttcgttg aagtcaaaaa aatatttcat cattttactg gcgctcgctg
    11701 caattgccgg actgggtact catgccgcct ggagtagcaa tggtttgcca cgtatcgaca
    11761 ataaaacgct ggccagactg gcacagcagc atccggttgt cgttttgttt cgccatgctg
    11821 aacgttgcga tcgttcaacc aatcaatgct tgtcagataa aacaggtatt acggtcaaag
    11881 gtacccagga tgcccgtgaa ctgggcaacg cttttagtgc tgatatccct gatttcgatc
    11941 tctattccag taataccgtc cggaccattc agtcggcgac ctggttttca gcgggtaaaa
    12001 agctgacggt agataaacga cttcttcaat gcggtaatga gatttatagt gcaattaaag
    12061 acttacaacg caaagcgcct gataaaaata tcgttatttt tacccataat cattgcctga
    12121 catatattgc taaagataag cgtgatgcga catttaaacc tgattatctg gatggtttag
    12181 tcatgcatgt ggaaaaaggc aaagtttatc tggatgggga attcgttaac cactaaattt
    12241 tcaatctgac agccagtgat ggctgtcatc attattacag aagacctttc aaacgtaacg
    12301 tttttcgggt ggcgacattc aaatcatagt gcgccagatc ttcaggtttt acccacgcgt
    12361 agtcttgaaa ctcttcgttt attttcactt ctcggttggc agaaacgcag tcaaaaatca
    12421 ggtaaatcat ataaatctct tccttgcgac catctgcata cgtcttggtg cgaatatcat
    12481 cgctgaaggt ccacggcgtg atttctgtca aaagcagctg ttctcccagc tcttcgcgaa
    12541 tttcgcggcg tagtgcctct tcaattcgtt cgccgggctc cacgccgcca cccgaaagcg
    12601 cccattgacc ggggaaaacg ccgcgatcgt cggccatttt acacagcaaa taagcaccat
    12661 cattttgaat caaagggcat acaatagtcc gttgtcgcac aggggtattc cttataaata
    12721 aaaagaaacc atgagaatac ggtaactcag aaatatgtga aagtgacagt aatctctttt
    12781 gcgatgataa ctgcact
//
LOCUS       DAZGHF010000134        12735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGHF010000134 DAZGHF010000000
VERSION     DAZGHF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.135"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            373..1863
                     /locus_tag="ACHK3A_004007"
     CDS             373..1863
                     /locus_tag="ACHK3A_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO8742028.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGESLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(1985..2557)
                     /locus_tag="ACHK3A_004008"
     CDS             complement(1985..2557)
                     /locus_tag="ACHK3A_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742029.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2643..2849)
                     /locus_tag="ACHK3A_004009"
     CDS             complement(2643..2849)
                     /locus_tag="ACHK3A_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8742030.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            3706..3930
                     /gene="hha"
                     /locus_tag="ACHK3A_004010"
     CDS             3706..3930
                     /gene="hha"
                     /locus_tag="ACHK3A_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO8742031.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(4262..4459)
                     /locus_tag="ACHK3A_004011"
     CDS             complement(4262..4459)
                     /locus_tag="ACHK3A_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742032.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            4658..5227
                     /locus_tag="ACHK3A_004012"
     CDS             4658..5227
                     /locus_tag="ACHK3A_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO8742033.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            5488..5889
                     /locus_tag="ACHK3A_004013"
     CDS             5488..5889
                     /locus_tag="ACHK3A_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO8742034.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTKHGHECPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            5877..6311
                     /locus_tag="ACHK3A_004014"
     CDS             5877..6311
                     /locus_tag="ACHK3A_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO8742035.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            7086..7312
                     /locus_tag="ACHK3A_004015"
                     /pseudo
     CDS             7086..7312
                     /locus_tag="ACHK3A_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(7314..8096)
                     /locus_tag="ACHK3A_004016"
     CDS             complement(7314..8096)
                     /locus_tag="ACHK3A_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFO8742036.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            8399..9319
                     /gene="rbsK"
                     /locus_tag="ACHK3A_004017"
     CDS             8399..9319
                     /gene="rbsK"
                     /locus_tag="ACHK3A_004017"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO8742037.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            9347..10663
                     /gene="fucP"
                     /locus_tag="ACHK3A_004018"
     CDS             9347..10663
                     /gene="fucP"
                     /locus_tag="ACHK3A_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO8742038.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            10675..11688
                     /locus_tag="ACHK3A_004019"
     CDS             10675..11688
                     /locus_tag="ACHK3A_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO8742039.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVKGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTTQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            12110..12394
                     /locus_tag="ACHK3A_004020"
     CDS             12110..12394
                     /locus_tag="ACHK3A_004020"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8742040.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(12610..>12735)
                     /locus_tag="ACHK3A_004021"
     CDS             complement(12610..>12735)
                     /locus_tag="ACHK3A_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO8742041.1"
                     /translation="LLITFLPVFLLPVLGDIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT         3317 a         2845 c         3191 g         3382 t
ORIGIN      
        1 gagtactcag cattcagcgc aggaactgtc atcttcttta ttttattctt cttcaggagg
       61 gcagttttct gaaagcgtta ctccggggaa actgtttatg tttattcccg gcaggaaacc
      121 cgtacctgca ataccctgga tgatgttttt tgctggtacg ttgttgtgtg aaaattaatc
      181 aacatgtgtg ctccggagag cgcccgtaga cgcacagatt cattctgaca ttctttagcg
      241 acaaaatacc gggtggttat tatttaacct gaaaatcgac agaaattttt atcctgtatt
      301 gattctgctg ctatttttgc tattagtagc aatagttgcg gtactttatg ttctgtgtgt
      361 tgtgaggtca ttatgccata ctttcagtat cctgatgagt ttcccctgag ctcgctgcct
      421 ccactcattc gtgatgccgt tattgaagct cagcaaatca cgcaggctcc cctggggctt
      481 gtggcagcat ctgccctggg cgctgtttca cttgtctgcc agaatctgat tgatgtctgc
      541 agactgaata cactgcgggg gccggtttct cttttcttac tgacgcttgc tgagtccgga
      601 gaacgaaaaa ccgcagtgga caaattatta atggagcccc tgtaccagca ggagatgctg
      661 ttatattcca gacacaaaaa tgaactgacg acatggaaaa ataaggaaga gcttttaaag
      721 gcgcagaaaa aagcattgtt gtcaaaactg aataaagaac tccgcaaagg cgcggatgag
      781 tcagaaacgc tcagacaact ggaagcgctt cagaaaaaca gaggggaaaa acctgtaaga
      841 tataaattca tttttaatga tgccacgaca gcagcaatta aagatcagct ttgtggtcag
      901 tggcgttcag tgggtatcat gtctgatgaa gccggtataa tatttgatgg gtataccctg
      961 tctgagctgc cttttattaa caaaatgtgg gatggctccg tattgtcggt ggacagaaag
     1021 aatgaacccg aacagatgat tgaaaatgcc agaatgacat tatccctcat ggtacaaccc
     1081 ggtctttttg accgatatat ggagcgaaaa ggttctgttg ccagggacag tggttttctt
     1141 gcccgttgtc taatcagtaa acctgccacc acgcagggga aacgctttat taacggagca
     1201 gtgaccccgg gagaatcatt aacggcattt catgaacgat taatggaact ggccagaggc
     1261 agtattgaaa aatccagtaa ggatgaacgt tattgtctgc atttttctcc agaagcgcaa
     1321 aaaatattta ttgatcacta taatgttctg gagcaggatt tgtctccttc cggaccctta
     1381 agccagtttc gggggcatgt ttcaaaaaaa acagaaaaca ttgcgagaat tgctgcgtta
     1441 ttgcagtatt tcagtcaggg tgaagggaaa atctctgccg atatcatgac gtctgctgtc
     1501 gtaataagtt cgtggtacac agatgaatat aaaaaattat ttgcgttacc tgatgaatct
     1561 gaactgcagc tgcaggacgc ccgggagctg ctcgactggc tcattgagga gtgccgcgga
     1621 gaatgcccgc cccgtgtcag gaaaaattat attctccagt gtggcccggg ccggttcagg
     1681 aacaggaaaa agctgaacgt cctgctgaac attcttgcat ctcaactcag gctctctgtc
     1741 gtgcaggaag gaaaaaccac ctatgtcctg ctttccgaaa taaacaatat cacgctgcct
     1801 gaactgaatg cgtgctacag ccagaaactg ataagatggc atgagcaatc ctttggctta
     1861 taatgttccc tgttgctgag acggattatt cagtggtggt atcgcagtat gcccgtcacc
     1921 ccccgcttct gcatgagggt gacggaggaa aagtaagtgt gcggaggccc gggagcgtta
     1981 tgaatcaaca ggcgtataac aggtactcgt caatcatcag ttccagctcg cgcttttccc
     2041 gtttgcgcgc cacagcccag ttttcaagct ctgcatgtga tttatgcttt aactcttcag
     2101 tccagggctg catggagtgg gcacacatca gatcatacca gcacagccat atcagcgtgt
     2161 ggtagagatc acgatcgtcg aaacgccgga gcaggctgtt cagcctgttt gccagccggt
     2221 tttcacccag acgatgtagc tcactgagta aaacgccgcc cagattaaaa tgtgtaagca
     2281 gcatgatttc atgggtctga atgctggtca ttaacccttc ctgaatggca ggaatgaacg
     2341 gtggtgttcc cgggttgacg acctgataga gtccctgtgg tgccactgag atgatagtgc
     2401 ctttttccgg cagaaaaaag tgggccaggg tcaggcgatg ataaccatga agatgcggag
     2461 accccattcc atgagcgacg ataagcctcg tcacgcttgt catccatttc tgatgctgga
     2521 tgagcagcag ctcacaccgg ttttcaagtt tttgcataat attctctcct ttagcgcgca
     2581 gcaaaatgcc tccgggggcc ggatcatgcc tttgctgcgg ttgaacggat aacggtcagg
     2641 aattacggac gggactgtgc aatacgcgcc tgcagccagg tttccacttc acttttcagc
     2701 catcgggagc tacggcccag tttgatgggg gcaggaaagg caccatcctt gatgagcttg
     2761 taaaaccatt tatcggtcag gccggtcagt tgagtgataa atgccatgtc gaccatctgg
     2821 tcatccatca gtgaaactgg ggtattcatg agaatgttcc ttcggatgtt gttaatctca
     2881 accggcgttg gtgatgaaaa caacgcacca ccttcaccgg cataacaacc ggggagaaac
     2941 gtaaaaaata atcgggaatg tgaccgtcag gagataaacg gggggattgc ggtggctgag
     3001 ccgtaccggt atgtcgggtg attgttgcga aacgatgctt ccgcacgcgc acgggaggac
     3061 tttcgtgaga caaggggaaa actggcgttc tgataattgc agagttgttt ttgaaggcgg
     3121 ttgtggtgca tcagcctgtg acctgtgggc aggcgacagg tatcactggt gggataagac
     3181 tgtctgattg gtattataaa tatataagag agctcaaaca catgagttca tatatgtgaa
     3241 ataaatctgt gagtgtgatc agcagaccag tctacccagt agcaacagat tgcgttgtac
     3301 tcacagggtt atccctgata actggcgtat aacctgcgta cataaaacgt acctgcaatg
     3361 cacctgaaca gagtgcatta agcatccgcc tgagcggtaa acgtatccga tctggtgagt
     3421 gacagtctga gagcattctc agtaatcgcc cccttgtatc ctggaaattg gttaagagcg
     3481 attagatttg gcaaggtgta tgcatccagc agcttaacac ctcagctaac tggctggtta
     3541 ccttcaggtt gtcgcaatgg catcggacat ccggtgtcgg ataactaaac gtagcgttaa
     3601 ggcgatattg ctgagagacc ggttaagtgg tggcacggga gtaatgagtt tggggagggt
     3661 accagaatgc cagtcaggtg cggtatgctg gcagtatcac gttgattgca gcataaagta
     3721 acgagagacg aatggaagtt aaaaccagag cagttccgtc gttgttcatc ccgggagacg
     3781 ctggaaaaag tgattacccg cacacgttat aaacttaccc ctgcggagct ggaagccttt
     3841 aactctgcgg tcgatcaccg gctggcagaa ctgacaatga acaaacttta cgatcgcgtg
     3901 ccggcttccg tctggaaata tgtcacctga atcttgaatc cgcgctacga tgactctggt
     3961 gttatctgtg caccggaaga tcgggaacat taagcagtat aacggcaaca aagaattcga
     4021 aatcaaaagg gaatggtgta cagaggggca ggcgttcagg cattaccggc ctgtatcttc
     4081 tgaaggggac tcgtatttgt cggggctgag tattccgctc gattcgatca cggattggca
     4141 gaaacctgac tgtgattgat tcaggtacca ttttgcaggg atccggtgcg ttactgtctg
     4201 gcacaacaaa cgcagcgggt cgctctgccc cgccgattcc tgataattcc tcccctgtaa
     4261 cctactgcaa cagtacaaat acactgacgc ctgaaacggt aaccagaatc agcaccagca
     4321 gtaacgtcag taaacagagc agacgatcgg tcttccggta ataaccgaag gggtcgacag
     4381 cgccacaata cgggcaacac actgtattat gagtaattgc gtttccacag tccctgcact
     4441 tctttttgcg tatcacgatc gctccctctc cacgccttat ccgtaacctg ttttttacat
     4501 taaaaaaaca acgccgggaa ttcaatcgtc agtccggaga cggccgttcg ggttatcaca
     4561 gagtgcctga gacagtgtcc tgccggaggt cacctcaggg gctctgcgca ttttttacga
     4621 cgtggctatt ccgtatgaac catacggaga tttaaccatg acctacaaat acaacccctt
     4681 ctggcagcaa cgtattcgtg agacggtgcg gcacgcactg gatgttcatc cccgcctgac
     4741 ggcacttcgg gttgatctgc gtctcccgga tgtgccggca gcaacggacg cggctgtgat
     4801 atcccgcttc atcaatgccc tgaaagcccg aatcgacgct tatcagaaac gtaagcatcg
     4861 ggaaggtaaa cgcgtgcatt cctcgaccct gcattacgcc tgggcccggg agttcgggga
     4921 gcttaaaggc aaaaaacact atcacctgct gctgctggtc aaccgggata cctggtgtcg
     4981 tgccggcgat taccgcgctc cggggtcact ggccgggatg attaaacagg cctggtgcag
     5041 tgccctggga gtggatgccg gacgttatga cacgctggca cattttcctg acaagccagc
     5101 ggtgtggctg gaacgcgatg atgaaaccgg ctttcagcag gtgctggaac gttctggcta
     5161 tctggcgaag gagcatacca aagctcgcgg caccggtgag cgcaattttg gctgtagccg
     5221 gagttgaaca cgacaggcgc accaataccc ggcaaaccaa aagtatgggt tgttgtctgg
     5281 cggcaacgat gtatccactc gcccactgac tgttttttac gtcagtgggc cattgtgtga
     5341 caaccacagt gctgtaacag caaccagcct gctttaccca aactccagtc cagattttaa
     5401 ctgaaccgcc attcacaccc tgaccacgct gcccgtaccg ggacggctgt tgcctgcgtg
     5461 tcgtctggcg gctgaggaac atatactatg tacgcaaaat cctttctcgc tcttgatggc
     5521 aacggacgtc tgacgggggc ccgtaccgca cagactgcgc cttacgaccg ctacacctgc
     5581 cacctttgcg gcagtgcact caggtaccac ccgcaatacg acactgaacg tccctggttt
     5641 gaacacactg acgacgggct gactaagcac ggtcatgagt gcccttatgt cagaccggaa
     5701 cgcagggagg ttcgcttgat taagcgtctg cagcaattcg taccggatgc cttacccgtg
     5761 gtgcgtaaag ccagctggca ctgcagacaa tgtcaccacg attattatgg ggagcagtac
     5821 tgcacaaact gccagaccgg aggattcagt attccccgga caactcagga ggaaatatgc
     5881 gaattctgaa ctgttatatg gcgaatgaca gcaaaggcca ttttgttacg gcgaaagaag
     5941 ctgcgaagca caaccgacag gacgttttgt gctgtgtgtc ctgtggatgc ccgttaacac
     6001 ttcagcgggg caatgacgga caaccaccgt ggtttgaaca tgaccagatg actgtcgctg
     6061 aaaaaatcct gctgcgatgt acctggcttg acccggcaga gaaagaggcc cgtcgtttgc
     6121 atctgcaggg catgacggtt ccggattata cggtgaaggt gagaaagtgg ttttgtgtga
     6181 tgtgtgacga agattatgag ggggaaaagt gctgcccacg ctgcggtact ggggtataca
     6241 gcagggcgtg ggggcggcag gaggtgccgt cggaagatgc cagggctgat aatccgttac
     6301 agaggctgta atggttgcct ccggagcagt ttgcggtaat gccgttcccg ggaatggatt
     6361 gccagtgcgg ggactgtgga gcttaattcc ggtgttggtg gcctttccgt tgattttgag
     6421 ccaacagccc tcctgtgact gacaggctgc ctcgtcattc cattcgtatc tccagtaaca
     6481 gggggttgta ttgtatttta atgtgccggt tcaggcgtgc gtttcccggc gcgtctgcac
     6541 cggcttaacc aaattcaaca gggataaaat agtgtaaata accgaagtat tacttgcaga
     6601 atcgggggaa gcatccgggg ttttattcaa caggcatggg aggctccggc tctgacagac
     6661 attgtactgt ggaatgttgt tacaggttgg ggcggcaacc gtgccaggag accatgagcc
     6721 gttcacggca ttaaaggcca cacttcggtc gtttacattc cctgtggtgg caggcatctt
     6781 ccgttaaggc gtcagagcgt taccaacgtt atgttgtcat ggctcacctt tcatagtcga
     6841 tgcttatctg gtgactgaaa ctagccgcag ataaagagtg tgaagttgat accatggagt
     6901 ggagtgacgt gcaggcagga gattcgctct atgcagggtt gtgagatgaa ttcaaaccgg
     6961 ctgtcagcgc cagtaatatt tgaagattcg tccggttgtt atccagtgtg cattaaaaac
     7021 ccagacatta tggacaatac aacggcttcc ataacaggga ggagcctgtt tattatccgg
     7081 gcgtaatgac taacctttgt gggctttttc ccgtcgtgct tttaacgttg aaggcatttt
     7141 ctgcctgccg attcaggaca gcgacaaaag ccatatcaga caattggtca atgatgacca
     7201 gcgtctggag tagatgataa gccaaattga taaactggaa tatgtgatga aagtgcaacg
     7261 taatcagtcc aatccgacga tgtttaacaa gagtacggtg ttttttcagt aagtcattcg
     7321 tcttctgaat caggtgtaat gatctttaca gaagcattcc ggcagtgcgt aacataatct
     7381 gccggtaacg gacgctcgct aatcagaaca tcaaatgcag agagcggtgc gaggctggca
     7441 gaggctactt catcgaacaa ggcgtggcgg gcgagcaaaa ttttccttag tccacgggcc
     7501 atcgctttac gcttagtggc aagatcttcg ggattaaacc agctgacgcc aaagtgatca
     7561 tgtacaccgc tggcggaaat aaatattttt cgcggattga gagagtctaa tggcgaagag
     7621 ttactggcat cgtaaaaagc atcacttctg gcacgatacg taccaccaca aagtattgct
     7681 gtcacattag gtttttcatt caacgcaaca aagacacgat gtgagtaaca gatgccagtg
     7741 aaggtgattg catccgggat catgcttata acgagcggta tctcctggcc attatcaaag
     7801 aagatcagat cattttcatt aaccattccg gcggccagaa ttgcaatagg taagtcatca
     7861 cgatgatttc tcggaacgtc ctggattact ggcatggatg gtgcgggttt atgcaccatt
     7921 acaatatagc cacccagtag ggtcagtggc agaggttcat cttcctgatg gagatcgcga
     7981 cgaatagtca ttacagaaac ttccagcatt cgtgccgcgt ctttcaaatg gattctgtcg
     8041 gttttcttaa gtatttcaat caaacgtcgg atacgctctt tttgcttcgt ttccatcaac
     8101 tctctccgta aaatgttata aaaataacat tatggcatta gtaaacattg ttttagttac
     8161 catactatac gaccgaccct ttgaaatgaa aagggtatag actgctgaat tttcggtgtt
     8221 aatcctgtta ggttgcttat gattagatag tgttggcctg agtgataaga ctggaagtct
     8281 attaaataaa catcgggttg atgtgctaat cgcgatctca gtcacaaatg atgcaaaagt
     8341 aacatgctaa tgttatttaa ataccattgt tatgcgaaag tttataagag gtagtgaaat
     8401 ggacattgca gttattggtt ccaatatggt ggatctcatt acttacacta atcagatgcc
     8461 taaagaagga gaaactctgg aagcacctgc cttcaaaatc ggatgtggtg ggaaaggtgc
     8521 taatcaagcg gtggcggcgg ctaaattgaa ttcaaaagtc ctgatgctga ctaaagtagg
     8581 tgatgatatt tttgcggata atactattcg taatctcgaa tcatggggaa ttaataccac
     8641 ttatgttgag aaagtcccct gtaccagcag tggtgttgca ccgatctttg ttaatgccaa
     8701 ctcgagcaat agtatattaa taattaaggg agcgaataaa tttctttccc cggaagatat
     8761 cgatcgagct gcagaagatc tgaaaaaatg caaattgatc gttttacaac tggaagttca
     8821 actggaaaca gtttaccacg cgattgagtt tggtaagaaa aatggtattg aagtgttatt
     8881 aaatccggct ccggcattgc gtgagttaga tatgtcttat gcctgcaaat gtgatttctt
     8941 tatcccgaat gaaacagaac tggaaatatt aaccggtatg tcggttgata cttatgatca
     9001 tattcgtttg gccgcacgtt cgttagttga taaagggctg aacaatatta ttgttaccat
     9061 gagcgaaaaa ggcgcattat ggatgacccg cgatcaggaa gtccatgttc ctgcattcaa
     9121 agtaaatgct gttgatacta gtggcgctgg tgacgcattt attggttgct tttcccatta
     9181 ttatgttcaa agcggtgatg ttgaagccgc tttgaaaaaa gccgctctct ttgccgcttt
     9241 cagcgtcaca gggaaaggta cgcaatcatc ttatccgagt attgaacaat ttaatgaatt
     9301 tttaacctta aacgaataat catcctgcac agttaaaggt agcactatga acgataaaaa
     9361 catcattcag atgcctgatg ggtatctgaa taagactccc ttgttccagt ttattttgtt
     9421 atcgtgttta ttcccgctat ggggatgcgc agctgcatta aatgacatat taatcactca
     9481 attcaaaagt gtattttcgc taagtaactt tgcttcagca ttggtgcaga gcgcatttta
     9541 tggaggttat tttttaattg caataccagc atcccttgtg attaaaaaga ccagttataa
     9601 ggtggcaatt ttaattggcc tgacgctgta tattggtggt tgtacacttt ttttccctgc
     9661 ctcgcatatg gcaacctaca ctatgttcct tgcagctatt tttgcgattg cgattggcct
     9721 gagtttcctt gaaactgcag cgaacacata cagttcaatg atcggtccaa aagcctatgc
     9781 cacattgcgg ctcaatatca gccagacctt ttatccgatt ggtgctgcgt ctggtatttt
     9841 attgggcaaa tatctggttt tctccgaggg ggaaagcctt gaaaagcaga tgtcaggaat
     9901 gaacgccggg caaattcaca actttaaggt gctgatgtta gagaacaccc ttgaaccgta
     9961 taagtatatg attatgattc tggttgtggt catggtgttg ttccttttaa cccgcttccc
    10021 aacctgtaaa gtggcacaga caagtcacca taaacgcccg tcagcaatgg atacgctgcg
    10081 ctatctggct agaaatccgc gctttcgtcg cggtattgtg gcgcagttcc tttatgtggg
    10141 tatgcaggtc gcagtatggt catttaccat ccgtctggcg ctggagttgg gcgatatcaa
    10201 cgaacgtgat gcttcaaact tcatggtcta tagctttgcc tgcttcttta ttggcaagtt
    10261 tatagccaat atccttatga cgcgttttaa tccggaaaaa gtattgattc tctattccgt
    10321 tataggggcg ctgttcctgg catatgttgc actggctcca agtttcagcg cggtctatgt
    10381 cgccgtcctg gtaagtgttt tgtttggccc ttgctgggcg acgatctatg caggaacact
    10441 ggatacagtt gataacgaac atacagaaat ggcaggagca gtcattgtta tggctattgt
    10501 cggtgccgcg gtggttccgg caattcaggg atatattgca gatatgttcc actcactaca
    10561 gctttcattc ctggtttcta tgctctgctt tgtctatgtc ggagtctact tctggcgtga
    10621 aagcaaagta cgtaccgcac tggctgaagt cacagcgtcc tgaggagaac cgttatgagt
    10681 acgcgtatta atttgtggcg ggcgcttttt ggcgagaaac ctcgtatctt gctggaaaat
    10741 agtgatttta ctgtcacgtc ttttcgatat gacagcggtg tcgaggggct taaaattgca
    10801 aacagtcgtg gacatttgat tattcttccc tggatggggc agatgatttg ggatgcgcaa
    10861 tttgatggac acggcctcac catgtgcaat atgtttcgcc agcctaaacc ggcgacagag
    10921 gtgatagaga cctatggctg ttttgctttt cattcaggtt tgctggctaa tggctgccca
    10981 tctgcggaag atactcatct gctacacggt gaaatggcct gtgccgcgat ggatgaagca
    11041 tggatggaat tagagggaga tatgttacgc cttaccgggc gttacgagta tgtaaagggg
    11101 ttcgggcatc attatctggc tcagccaaca gtggtccttc acaaatcctc tacactgttc
    11161 gatattaaga tggccgtaac caatctggct tctgtagata tgccactaca atatatgtgc
    11221 cacatgaatt atgcatatat accaaatgct acgttcagtc agaatatacc ggacgaaata
    11281 ttaaggttgc gtgaatcagt accttcgcat gttaatccca ccactcagtg gctggcgttt
    11341 aatcaacgga ttatgcaggg agaagcctct ctgtcgacgc tgaatcaacc ggagttttat
    11401 gatccagaaa ttgtcttctt tgcagacaaa ctggacgctt acacagatca gccagagttt
    11461 cgcatgatcg ctcccgatgg cactacgttt gttactcgtt tctccagtgc cgaactaaat
    11521 tatgtgacac gctggatcct ttataacgga gagcaacagg tagccgcctt tgcattacct
    11581 gcaacctgtc ggcctgaggg gtatcttgcc gcgcaacgta atggcacgct gattcaggtt
    11641 gcaccgcagc aaacgcgcac ttttacagtc accacaggaa ttgaataacc actcaaggcc
    11701 tgaacagcgg cgcgcttatc gttaaggtaa gcgcgttttt ttacccgcca ggacaagact
    11761 atgatagcgt tgcccttata gacgatcaca ttatcgtcca ctccaaattt gcgcaactac
    11821 tgtagagtgg tgtactccac agcttctttg aggagcttaa cctccattgt tttcttaccg
    11881 agcaagcact gcaactcttt tatttatttg tagtggtcaa caaaaactgg tcacaatttc
    11941 agtttttttc cagtatctat tttccgattc gttcggcggt aatccaccgt tatattcatg
    12001 aggccggagt gcactataaa agtgtatgcg taaaccgact gctgtatgta gccattaagc
    12061 cagtattggt aacataggta cccacctttt caactagtgg acacctgtta tggaacaaaa
    12121 aacattatca gctgaacccc gcaggtcatt ttcaaatgag tttaaacttc aaatggttaa
    12181 actggcttta caaccaaggg gttctgttgc ccgtattgcc cgggagcatg atatcaatga
    12241 taatcttctg ttcaaatggc tcaggctctc gctgatcaaa gggcgtatat cacggcgtct
    12301 tccggtaacg aactcttctg gcattggcgt tgaattatta cctgtggaga tgacgctgga
    12361 tgagcggcct taccccgttt tttacgcgag gtaattatga agatcaatga ctagccgtcg
    12421 aaccgtgtgc atgaactgct gcactgaaac ttctcagctg taaaataatc cttacgctac
    12481 gtacttcccg gagcgcttac gattaatcgg atgcagtcct gactggaaga aagaaaaatg
    12541 gagaaaataa gaggaattaa tatcctccac gttcctgaaa tcctggagga tattaatgtc
    12601 atcaaaatat tagcgaacga taatccctaa caaaataccc agtgcgccaa agtcagcatc
    12661 actgaaggtg gtgttagcaa aaccaatatc acccagcacc ggcaataaga atactggcag
    12721 gaaggtaata agcaa
//
LOCUS       DAZGHF010000135        12604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGHF010000135 DAZGHF010000000
VERSION     DAZGHF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.136"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2764
                     /locus_tag="ACHK3A_004022"
     CDS             <1..2764
                     /locus_tag="ACHK3A_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO8742042.1"
                     /translation="DWMVNGLAGDALHKIGKGTLVVAGSGENPGTLNTGDGTVILAQK
                     ADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDIAFNRLAA
                     ADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMDGGADI
                     SGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESRTFSI
                     GQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGTSAVG
                     GASGSTTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTSSALTV
                     SDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGSGEYDF
                     MTGAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFHTLTA
                     GSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLVTAPA
                     GTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATRQDAETPN
                     PVEAVSPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVNSSAV
                     RDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNYTH
                     VQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVGAGLYASALFDSGAYID
                     LIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAELVYGAV
                     SGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLAN
                     GETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDNAVNA
                     SFRYSF"
     gene            complement(3332..6004)
                     /gene="rcsD"
                     /locus_tag="ACHK3A_004023"
     CDS             complement(3332..6004)
                     /gene="rcsD"
                     /locus_tag="ACHK3A_004023"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO8742043.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTTPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLTNQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            6743..7873
                     /gene="ompC"
                     /locus_tag="ACHK3A_004024"
     CDS             6743..7873
                     /gene="ompC"
                     /locus_tag="ACHK3A_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO8742044.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNGTYVSDNNVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            7985..9040
                     /gene="apbE"
                     /locus_tag="ACHK3A_004025"
     CDS             7985..9040
                     /gene="apbE"
                     /locus_tag="ACHK3A_004025"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO8742045.1"
                     /translation="MEISFTRVALLAAALFFVGCDQQPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            9114..10178
                     /gene="ada"
                     /locus_tag="ACHK3A_004026"
     CDS             9114..10178
                     /gene="ada"
                     /locus_tag="ACHK3A_004026"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO8742046.1"
                     /translation="MKYATCLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSIINAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVRDVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            10178..10828
                     /gene="alkB"
                     /locus_tag="ACHK3A_004027"
     CDS             10178..10828
                     /gene="alkB"
                     /locus_tag="ACHK3A_004027"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO8742047.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            10904..12547
                     /gene="yojI"
                     /locus_tag="ACHK3A_004028"
     CDS             10904..12547
                     /gene="yojI"
                     /locus_tag="ACHK3A_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO8742048.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         3048 a         3012 c         3438 g         3106 t
ORIGIN      
        1 tgactggatg gtgaacggac ttgcgggaga cgcacttcat aaaattggta agggaacact
       61 ggtggtggcc ggcagtggtg aaaatcctgg caccctgaat acgggggacg gaacggtcat
      121 tctggcacag aaggctgatg ctgcaggcag ggtccgggca ttcagtgaag tcagtattgt
      181 cagcggacga ccagttgtgg tgctgcagga cagtcatcag attgaagggg accgcattcg
      241 ctggggatac cggggaggta cgcttgatat caacggaaat gacatagcct tcaaccgcct
      301 ggctgcggct gatgaggggg cagtactcac aagccgggcg cgtcctgcca cagtccgtct
      361 ggatttcagc cagtcagggc agaaggccgt gatgtggcat ggtcatttta ccggtaacct
      421 cagcgtcctg aacaacacgt catctgcggt ggattttatc atggatggcg gagcggatat
      481 atccggcagt tttacacagc agggcggggg actgtatatc caggggcatc cggttgttca
      541 tgctgtcagc agtgaggcgg ttgcgactgc attgcgaaag cagggtgata attctgttct
      601 gacgcagccg gtatcatttg ctcagaagga ctgggagagc agaacgttca gcatcggtca
      661 actgaaactg aagggggcgg cattcagcct ttcccgcaat gcgacactga cgggagatat
      721 tgatgctgat aatgccacca tggtgctggg aagtgactcg ctatatctgg atatgaagga
      781 cggcacgggg agcagcagtg cgccggttaa ggggacgtct gccgtgggcg gtgcatccgg
      841 tagcactaca tttcgcggga atgtgaatat gcgtcattct gccctgacgg tccgcgacca
      901 ttttactggc agtataacgg catcggacag ccggatagct gtgagttcag agaatgtgcg
      961 tctggaaggg aattcccgcc tgacctcttc tgcacttact gtcagtgacg gcgggcgttt
     1021 acatgtgaag gggggactgg agacagatgg tggtgtgaca ctggacagag gaaccctttt
     1081 ggtggacggg ggaagtgtca ggaatgatgt atatgaacgt ctgctggcct ggtcagagga
     1141 gcgtggcggg ctgaacggaa gcggtgagta tgattttatg acgggggcag ctggtctcct
     1201 gcggagttat gtcagaggga gtgcaggtaa tgtgaatctg cagaatgcgg cctggatgat
     1261 gacgggaaac tcatccgtga agcatcttga gtcatcggga agtgctctgt atttcagcag
     1321 gccgggcggg gagttccata ccctgacggc aggcagtatg gatatcagcg acagtgttct
     1381 ggtgatgcgg acggacctta accactcaga ccagcttcgg gtgacagaat cgctgcgggg
     1441 taagaataac ctgttgttgg ttgattttac tgagcgttct gacgggcaga aggcgctgaa
     1501 tatccctctg gtgacggcgc cggccggtac cggtgcggat gtgttcagtg tgaaaacgcg
     1561 tgataccggc ttcagtcata tcacacctgt tgtcagggct gaacagggga cgggcggcac
     1621 ggcctggcag ctgaatgttg tgcagccgga aactgccgca gagcctgtgg cgacgcggca
     1681 ggatgcagag acgccgaatc ctgtggaagc tgtatcaccg ctcccttcac cggtttctgc
     1741 tccgtcatcg gctccggagg cgtcagtgac ggatgtactg accggagaga atgcggggga
     1801 atcaggtgag taccgtgatg agacccggtg gctgctgaca ggataccgga gtacggttaa
     1861 tagctccgcg gtcagggatg cggggatgct gatgtcaatg gggcaccgta acttcattaa
     1921 tgaggtcaac aacctgaaca aacgtatggg tgacctgcgg gacatcaacg gcgaggccgg
     1981 tgcatgggcc cgtatcatga gcggaaccgg ctctgccggc ggtggcttca gtgacaacta
     2041 cacacacgtt caggtggggg cggataaaaa acacgaactg gacggtctgg acctcttcac
     2101 cggggtgacc atgacatata ccgacagcaa cgccggcagc gacaccttca gtggtaaaac
     2161 gaagtcagtg ggtgcgggtc tgtatgcttc agccctgttt gattccgggg cctatatcga
     2221 cctgattggt aagtatgttc accatgataa tgagtatacg gcgtcctttg ccggactcgg
     2281 cacaaaagac tacagctctc attcctggta tgccggtgct gaagtgggtt accgctatca
     2341 tgtcacagag gatgcctgga ttgagccaca ggcggaactg gtctatggtg ctgtctccgg
     2401 gaaacagttc agctggaagg accagggaat gaacctcacc atgaaggata aggactttaa
     2461 tccgctgatt ggccgtaccg gtgtggatgt gggtaaatct ttctccggta aggactggaa
     2521 agtgacggca cgtgctggtc ttggctatca gtttgacctg ctggcgaacg gagaaacggt
     2581 actgcgtgat gcatccggag agaagcgtat taagggcgag aaagacggcc gtatgctgat
     2641 gtctgtgggt ctgaacgcag aggtccgtga caacatccgt tttggtctgg agtttgagaa
     2701 atcagccttt ggtaagtaca acgttgataa tgctgtcaac gccagcttcc gttattcatt
     2761 ctgacggttg ttactgaagg ggattatggc ctgacggctc cctgtaaata aagacaggca
     2821 cagaagattg tgagttactc agtgttccac caaaaagtaa ggtcgcaact ctgtgcctgc
     2881 ttcgtaagtg tgcagtgagg gccatatggt tataactgaa gcatccgacg acgtagtgtc
     2941 taccagagtg tcagatatgc aggaaaatat gagttccgac agaaatgcag taaaaaaagg
     3001 agtctcgcac tcccctccat aaaactccag ggctgtaagt tgtattaaaa agctgatttt
     3061 tctttagcga tacagacctc aataagtata acacccttca gttactgtgg ttatgcacag
     3121 gtgttatttt taacagtgtg gataaacagt gtcgattatt cagattggtt atggggcatg
     3181 tatgggacat ttttaatcgg cgaaatacgt cgaagttcgt cgatatggta aacgaatcat
     3241 ctatccaacc cttgaaaaac ggcgctcctg gacgatcttc gtcgattttt aaaatgcaac
     3301 aagtacgttg ttcatgtaat aggctacctt gctacagcaa gctcttgaca taactgtcaa
     3361 tgtcgctgat gtatttttct attcctggaa catccttctc acgaatcaga tgttccagcg
     3421 tttcacataa ctgcttgccg ggtaccagat ttagcatggc aaatacgcct ttaagacgat
     3481 gagccgtttg ggctaacgca gcaaagtcac tggtcgctgc ttcagtatac agcctcttaa
     3541 catcatccgg tactgtgtct acaaagagcg cataatagcc gctggcatgg agttgcgcat
     3601 tttcatctcc gcccagaggc gattctgtca cctcttcctg cgccagttgc acttcaatta
     3661 attgtaagac cgcttcctgc atagcgttgc tcatattgaa gttgacgcac aattgaccag
     3721 gcccaatttc ccgtacgcca gactcatcat cgcttaaaag caagccagag gcagtaagat
     3781 tagacggatt atccgttaaa aagatatcat aatcttgact aattaatctt tcatcgggtg
     3841 tgatacaggt tgcaccccaa ttttctaact ggcgagtgac aatattccga atttctgccg
     3901 aagtaacatc caccattacg cagacatcat ccagtaaacg ctcttcttcc tcttcaactt
     3961 ccgggtcagc tgcgagcatt ttgatatgca cagagtagcg agtaccaagc ccatcccgcg
     4021 ttttgatgtt taaatgaccg cccagtttac gcgccagttg atcgctcagc cagaatgcca
     4081 gcgggtccgc cttgccatag cgatcgtttt gggtctggtt gataaacggg aagtgcaaat
     4141 tatccatttc atgaatgctt acgccttctc cagtgtccag aatacggaac gtcaggcggt
     4201 cttcggagga ctcatcctga tcaacctcaa gggtgatttt tcccaattgc gttgaggtca
     4261 cggcatattg catcagtaac agcaaaatac gtcgtaaggc atcgcgatcg ccgcggcgca
     4321 tgtcgtgtgc tttcagatga ttgttaatca gcagttgcag acctttacgc ttgatggcag
     4381 gcaacactga aggcacaact tcatcaatta aatcctgcac ggagaacagc accgtctcac
     4441 ttttccagct atcgtccgca agcatgttcg ctaactgtat ttcatcgacc agccgcacca
     4501 gcacatccgc ctgattcgtc agttgtttgc cttccggggc gttgagttta gccgcgctct
     4561 ccgccaggga ctgtgcgggt tctttcagcg catcgccaat gtttttcata aaggtcatcc
     4621 gcccctgctg gtttttctca tacagacgct gcgcctgctt gagtttcttg tttaccagca
     4681 cttcgcgatc ctgatcgcga ataatgaaaa tttgtgtgcg cggcgcaacc tggctgcgga
     4741 acatgcggat ctcatacagc tcgttattga tcgtcgcctg aataatcccc tgatgctgtt
     4801 ccgccatggt ggtgatgttt tgcagattca aatgcggcag caaatgatcg gcaattttgt
     4861 tacttatgac agtgcggttc gattcctgat cgtgaaccag caggccgagc ggcagcagtg
     4921 agactatctc ttcattgatt gcccgtaaaa tgcgcaattc gttattgacc gccgtgttgg
     4981 gtagactttc tgtactgcga ctggagaaat ggcgaaatgt ggtatagcca aataacgcca
     5041 gcgccagcaa accgatgttc agcagcagtg gcagcagaat gttttgcaac gtatccagca
     5101 ataaggtgcc ataaggaacc tgccagacca ggcgcatatc ggtagagttg agtgccgagg
     5161 agatttcaat cttcgtactg ttaaagtgga tactgacgct atccgtccct tctttctcat
     5221 tatcattgtc tcccgttgcc gtcgcgtctg gctcaaggcg gaaactgtcc agcggcatac
     5281 ccggtgggat caaatcatta atcggcagat caaaagccac aaccgttgcc agatgtcccg
     5341 gctggttgaa cgtggtacgc aaggtaaagt aatgaccgtt ctgccaggcc aggcggcgca
     5401 tgttagaaaa gctttcgcgt tcatcgaggg cgttggcctg ttgcaacatc tctgcacgac
     5461 gtgaatcaac aatgtcgctg acggtcgatt ctttaaatcc ggaggtgaga tctttgaggg
     5521 gcagggttga gatcagcacc agactgttat cctgaccatt caggtaatac atcgaccacg
     5581 gtacattttc tgcgccccac aatgtatcca gataagtgga catccgttga gtcatctcaa
     5641 gcgttgagtt gtcgtgagag ccaaagatta acgcttccgt tttgcggcgt ggtttctcca
     5701 gatagtagac atcctgtttc aggcgcgtct cttgtaaacc ttcgccggag gagggggtgg
     5761 tcgtcgcggc aatgttgtcg tagatctgcc aggtcacgta acgccaggta tcgacgcgct
     5821 tttggatagc gtgggtaatg tcgacaatct ggtaactttt atctttcagc caggcgttaa
     5881 cggcgctttg taccattaca cccatcgtca ccagtaacac aatgatcaac agtaaaaaga
     5941 agcgggtaat gctccccggt aggagtgaaa agcgggtcgt ggccgttgtc tctttctgac
     6001 gcattcgtgt ttatgacctg ttaaaacttc gcgaagtgat agggcagtat aaagggtaca
     6061 gtgtgaaatt ccagactctt accggattga tgattctcta ttctcccgtg atatttatca
     6121 tcctgcattg ggggaaatag atgtacaagc gccattttgc tgtacaaata acctacaaaa
     6181 agcctaacga aattaattgc aaataaacga agattgctgg aaattatgct gatgttaatt
     6241 atttgtgaaa tagttaacaa gcgttatagt ttttctgtgg tagcacagaa taatgaaaag
     6301 tgtgtaaaga agggtaaaaa aaaccggatg cgaggcatcc ggttgaaata ggggtaaaca
     6361 gacattcaga aatgaatgac ggtaataaat aaagttaatg atgatagcgg aagatattct
     6421 agttgcgagt gaaggttttg ttttgacatt cagtgctgtc aaataattaa gaataagtta
     6481 ttgattttaa ccttgaatta atattgcttg atgttaggtg cttatttggt cattccgcaa
     6541 taatcttaaa aagttccctt tcatttacat tctgaaacat ctatagcgat aaatgaaaca
     6601 tcttaaaagt tttagtatca tattcgtgtt ggattattct gcatttttgg ggagaatgga
     6661 cttgccgact gattaatgag ggttaatcag tatgcagtgg cataaaaaag caaataaagg
     6721 catataacag agggttaata acatgaaagt taaagtactg tccctcctgg tcccagctct
     6781 gctggtagca ggcgcagcaa acgctgctga agtttacaac aaagacggca acaaattaga
     6841 tctgtacggt aaagtagacg gcctgcacta tttctctgac gacaagtctg tagatggcga
     6901 ccagacctac atgcgtcttg gcttcaaagg tgaaactcag gttactgacc aactgaccgg
     6961 ttacggccag tgggaatatc agatccaggg caacagcgct gaaaacgaaa acaactcctg
     7021 gacccgtgtg gcattcgcag gtctgaaatt ccaggatgtg ggttctttcg actacggtcg
     7081 taactacggc gttgtttatg acgtaacttc ctggaccgac gtcctgccag aattcggtgg
     7141 cgacacttac ggttctgaca acttcatgca gcagcgtggt aacggcttcg cgacctaccg
     7201 taacaccgac ttcttcggtc tggttgacgg cctgaacttt gctgttcagt accagggtaa
     7261 aaacggtagc gtagatggcg aaggcatgac caacaacggt cgtggtgctc tgcgtcagaa
     7321 cggcgacggc gttggcggtt ctatcactta tgattacgaa ggcttcggta tcggtgctgc
     7381 agtgtccagc tccaaacgta ctgacgctca gaatggtacc tatgtaagcg acaataatgt
     7441 ggtccgtaac tacatcggta ctggtgaccg tgctgaaacc tacactggtg gtctgaaata
     7501 cgacgctaac aacatctacc tggctgctca gtacacccag acctacaacg caactcgcgt
     7561 aggttccctg ggttgggcga acaaagcaca gaacttcgaa gctgttgctc agtaccagtt
     7621 cgacttcggt ctgcgtccgt ccctggctta cctgcagtct aaaggtaaaa acctgggtgt
     7681 catcaatggt cgtaactacg acgacgaaga tatcctgaaa tatgttgatg ttggtgcgac
     7741 ctactacttc aacaaaaaca tgtccaccta tgttgactac aaaatcaacc tgctggacga
     7801 caaccagttc actcgtgacg ctggcatcaa cactgataac atcgtagctc tgggtctggt
     7861 ttaccagttc taatctcgat tgatatcgaa caaagggcct gcgggccctt ttttcattgt
     7921 tttcagcgta caaacacagt tttttggtgt actcttgcga ccgttcgcat gaggataatc
     7981 acgtatggaa ataagcttta cccgcgtggc actgctggct gccgcgctct tctttgttgg
     8041 ttgcgatcaa caaccacaac ccgccaaaac ccacgctact gaagttaccg ttcttgaagg
     8101 caaaaccatg ggtaccttct ggcgtgccag catcccgggc attgacgcca aacgcagtgc
     8161 cgaacttaaa gaaaagattc agacccaact ggacgctgac gatcagctgc tttcgaccta
     8221 taaaaaagat tccgcgctga tgcgctttaa cgactcgcaa agtttgtcgc cgtggccggt
     8281 aagtgaagcg atggccgata tcgtcactac ctcgctgcgc attggcgcga agaccgatgg
     8341 cgcgatggat ataaccgtcg ggccgctggt gaatctgtgg ggctttggtc cggaacaaca
     8401 accggttcaa attccgagcc aggaacagat cgatgcgatg aaagccaaaa ccggcttaca
     8461 gcacctgacg gtcattaatc agtcgcatca gcaatatctg caaaaagacc tgccggattt
     8521 atatgtcgat ctctccaccg tcggcgaagg ttatgcggcg gatcacctgg cacgcttgat
     8581 ggagcaggaa gggatttccc gctatctggt gtcggtgggc ggcgcgctga acagccgtgg
     8641 tatgaacggt gctggcaagc cgtggcgggt agcgattcaa aagccaaccg ataaagaaaa
     8701 cgcggttcag gccgtggtgg atatcaacgg tcatgggatt agtacctctg gcagctaccg
     8761 taactattac gaactggacg gcaaacgtct ttctcatgtt atcgatccac aaaccgggcg
     8821 tcctatcgaa cacaatctgg tatccgtgac ggtgattgcc ccgacggctc tggaagccga
     8881 tgcctgggat actggcttaa tggtactcgg accggagaaa gccaaagagg ttgttcgccg
     8941 ggaaggactg gcggtctaca tgatcaccaa agagggcgat aattttaaaa cctggatgtc
     9001 gccgcagttt aaaagcttcc ttatcagcga aaaaaattaa agcgcaagat tgttggtttt
     9061 tgtgcgatgg tgaccgggca gactaaaggc tatccttaac cagggagctg attatgaaat
     9121 atgccacatg cttaactgac gatcaacgct ggcaatctgt cttagcccgc gactcgaatg
     9181 ccgacggcga attcgttttc gccgtgcgca ccacgggtat cttttgccgt ccgtcttgcc
     9241 gagccagaca tgccttgcgg gaaaatgtct ccttctacgc aaatgccagc gaggcgcttg
     9301 ccgctggctt tcgtccctgc aaacgttgtc agccagacaa agccaatccc ctgcaacatc
     9361 gccttgataa aattacccac gcctgccgac ttctggaaca ggaaacgcct gtaacgctgg
     9421 aagccttagc cgaccaggtg gcgatgagtc catttcattt gcatcggttg tttaaagcga
     9481 ctaccggaat gacgcctaaa gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat
     9541 tgctggcgaa aggggagagc gtgactacat ctattattaa cgccggattc cccgacagca
     9601 gcagttacta ccgcaaagcc gacgaaacgc tgggcatgac ggctaaacaa ttccgtcacg
     9661 gtggcgaaaa tctggcggtg cgttacgcgc tggctgattg tgagctgggc cgttgcctgg
     9721 tggcagaaag tgagcggggg atttgcgcga tattactggg cgatgatgac gcgacactaa
     9781 tcagcgagtt acagcagatg tttcccgctg ccgacagcgc gcctgctgat cttacgtttc
     9841 agcaacatgt gcgtgacgtg atcgccagcc tcaatcaacg cgatacgccg ctgacgttac
     9901 cgctggacat tcgcggcact gcttttcagc aacaagtctg gcaggcactg cgcacgatac
     9961 cttgcggtga aaccgtcagt tatcagcaac tggctaatgc catcggcaaa ccgaaagcgg
    10021 tacgggccgt tgccagcgcc tgtgccgcca acaagctggc tatcgtaata ccctgtcatc
    10081 gggtggtccg tggtgatggc acactttccg gttaccgctg gggcgtgtcg cgcaaagcgc
    10141 aactgctgcg ccgcgaagct gaaaatgagg aaaggtaatg ttggatctgt ttgccgatgc
    10201 tgaaccgtgg caagaaccac tggcggctgg agcggtgatt ttgcaccgtt ttgcttttaa
    10261 cgctgcggag caactgatcc gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca
    10321 gatggtcact ccaggcggat ataccatgtc ggtggcgatg accaactgtg ggcatctggg
    10381 ctggacgacc catcggcaag gttatctcta ttcgcccatt gatccgcaaa caaataaacc
    10441 gtggcccgcc atgccgcaga gttttcatca tttatgtcaa cgtgcggcta cggcggcggg
    10501 ctatccagat ttccagccag atgcctgttt gatcaaccgc tacgctcctg gcgcgaaact
    10561 gtcgctgcat caggataaag acgaaccgga tctgcgcgcg ccgattgttt ctgtttctct
    10621 gggcttaccc gcgatttttc aatttggcgg cctgaaacga aatgatccgc taaaaagatt
    10681 gttgctggaa catggcgatg tggtggtatg gggcggtgaa tcgcggctgt tttatcacgg
    10741 tattcaaccg ttgaaagcgg ggtttcatcc actcaccacc gactgccgct acaacctgac
    10801 attccgtcag gcaggtaaaa aagaataaaa ataagaatta ttattgctgt gcgcgcgaag
    10861 atgtttaaac tgcgggctgt aattcattgt ccgggttttc tgcatggaac ttcttgtact
    10921 tgtctggcgg cagtatcgct ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc
    10981 ggcattaggc attggcttaa ttgcttttat caatcagcgc cttatcgaaa cggcggatac
    11041 cagtctgctg gtattgccgg agtttctggg gttattgctg ctgttgatgg ccgtcactct
    11101 cggatcgcaa ctggcgctca ccactttggg gcatcacttc gtttaccgac tgcgtagtga
    11161 atttatcaag cggattctcg atactcacgt cgagcgcatt gaacaactcg gtagcgcctc
    11221 gttgctggcg ggattaacca gcgatgtgcg caatatcacc attgcttttg tgcgtctgcc
    11281 ggaactggtg caggggatca ttctcactat cggttcagcg gcgtatttgt ggatgctgtc
    11341 gggcaaaatg ttgctggtaa cggctatctg gatggcagtc accatctggg gcggttttgt
    11401 gctggtggcg cgggtgtaca aacatatggc gaccctgcgt gaaaccgaag ataagctgta
    11461 cacggatttt caaactgtac tcgaagggcg caaagagctg actctgaacc gtgaacgcgc
    11521 cgagtatgtg tttaacaacc tctacattcc tgatgcgcaa gagtatcgcc atcatattat
    11581 tcgcgcagac accttccatc ttagtgccgt gaactggtca aacatcatga tgctgggcgc
    11641 tatcggcctg gtgttctgga tggcgaacag tctcggttgg gctgatacca acgttgctgc
    11701 gacctattcg ttgacgctgt tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt
    11761 gccgacgctg ctgacggcgc aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc
    11821 tttcaaagca gagtttccgc gcccgcaggc ttttcccaac tggcaaacgc tggagctgcg
    11881 taacgtgacg tttagctatc aggataacgc gttttccgtt gggccaatta acctcaccat
    11941 caaacgtggc gagctgctgt ttctgattgg tggcaacggt agtggaaaat cgacgctggc
    12001 gatgttgctg acgggcttgt accagccaca aaatggcgaa atcttgctgg atggcaaacc
    12061 tgtcagcggc gaacaaccgg aagattatcg aaaactgttt tcggcagtgt ttaccgatgt
    12121 ctggctgttt gatcaactgc tggggccgga ggggcaaccc gctaacccgc aactggttga
    12181 gaagtggctg gcgcagctga aaatggctca taagcttgag ttaagcaacg ggcgtattgt
    12241 taacctgaag ttatcaaaag ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga
    12301 agaacgcgat attatcctgc tggatgaatg ggcggcggat caggatccac acttccgtcg
    12361 tgagttttat caggtgctgc tgccgctgat gcaggagatg ggtaaaacta ttttcgccat
    12421 cagtcatgat gatcattact ttatccacgc cgaccgcctg ctggaaatgc gcaacgggca
    12481 acttagcgag ctgacaggcg aagagcgcga tgctgcttcg cgtgatgccg ttgcccggac
    12541 ggcataatat tacgacgctg acctggcgga ctgctgccgc caggttaata tgctaaaaat
    12601 ccct
//
LOCUS       DAZGHF010000136        12204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGHF010000136 DAZGHF010000000
VERSION     DAZGHF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.137"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..287)
                     /locus_tag="ACHK3A_004029"
     CDS             complement(<1..287)
                     /locus_tag="ACHK3A_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742049.1"
                     /translation="MKKVNVVGYTELIFGAISEKTEIKSKKLKRRCNNLIEKETPSRI
                     FKFTNFLNCGVQHLFVLHRMKTNIPYKTNDDMKKSTGGIITGSKFIGLIIV"
     gene            complement(640..867)
                     /locus_tag="ACHK3A_004030"
     CDS             complement(640..867)
                     /locus_tag="ACHK3A_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8742050.1"
                     /translation="MEIENTPENIKRLRKKIGLTQKECAEMFGMTARSWRRKEEPTGT
                     VSGSKLSPVEFEYILLLAGEHPEYVLSKRDA"
     gene            complement(857..1117)
                     /locus_tag="ACHK3A_004031"
     CDS             complement(857..1117)
                     /locus_tag="ACHK3A_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742051.1"
                     /translation="MKMRKGDRQYYLNKEGDTFRLVKRVKTFSKSATLGKTKATVKTV
                     ADIIFREEEFGSIDFTQDGLREGDKEIISIMLKNIKRDENGN"
     gene            complement(1308..3569)
                     /locus_tag="ACHK3A_004032"
     CDS             complement(1308..3569)
                     /locus_tag="ACHK3A_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFO8742052.1"
                     /translation="MRLWIAEKPELGKLIAQVLGNPQYFDGYIVCNNDTVSWAIGHLL
                     KLIPPELKTPEYKSWSKNTLPLQLRPLQYIVIPKTEKQFNVLSGLITRADMIIHAGDP
                     DEEGQLLIEEILSYCGNKAPVKRVLINDLNKDAVRQAMRNLQDNRAFYGLYQKALARS
                     AGDYLFGLNMTRAYTIAAREKGHNEILSVGRVQTPVLGLVVRRYEENRDYKKYDYWCV
                     QAEFIDTDTIISPMLVPPEDAPVDDKGRIISEQYCDEVIRSCKGQQASVSLAESEIKS
                     RRPPLPFSLLDLQVKMNRQYGFTAEKVLEITQKLREEYRAITYNRSDCRYLSEEQFSQ
                     SPATLAMLSSFFPELGKFFSHIDSGRKSRAFNDKKISAHTAIIPTPTPTGLDQMNEDE
                     MRVYKAIVAQYLVQFMPDKTFDSAVVEFNINGFLFRQRAIRPVSPGWSILLADRDEET
                     LPDCKLFDAITSLTKGQQLVCSSVNSRKETSTPPPLYTEASLLEDLRRVARYVEDPRI
                     RQLLLDRDEGKEEERGGIGTPATRGHILNVLQVRGFIAVKNKKVVPTELGLSFIHALP
                     EVMTRPDMTALWHEQQSMIETGEISIDDFLDELEQFILQQVSDVDISGLAVAEYQCTC
                     GGRYVRRRTDKGFFWGCSKYPDCRNTVPDRGGVPDFSRSEFERKVRCPVCGSGMKISP
                     KAYNCSNERCGFRLWGTQFGKELTLAQATDLLMKGHTREIKGLKKKDGAKFDAVLNLN
                     RDGSVVPTFKKKK"
     gene            complement(3610..3843)
                     /locus_tag="ACHK3A_004033"
     CDS             complement(3610..3843)
                     /locus_tag="ACHK3A_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO8742053.1"
                     /translation="MENKYRKYPKSSKQEYLFQLRKCSKLSTLDKIIQRKAYTMNDID
                     FVEFLTAADHRLAEIVTGQLFDKIPKNVWKLIY"
     gene            complement(3893..4249)
                     /gene="ssb"
                     /locus_tag="ACHK3A_004034"
                     /pseudo
     CDS             complement(3893..4249)
                     /gene="ssb"
                     /locus_tag="ACHK3A_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(4261..5526)
                     /locus_tag="ACHK3A_004035"
     CDS             complement(4261..5526)
                     /locus_tag="ACHK3A_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8742054.1"
                     /translation="MGKLTLVEQALRLHKEYYGDECFGTIVKNYDYYPKVAEIFINRK
                     NKNTYSNLDIEPVASSGVVHTGSNPVMVKVGRTKKLMFKDGSGAFRIHLGKNEVVHIV
                     RYIHNSSIRMEYAIGTTKGLFMLHNLCEAGRVKLNRSLPRKGIWKIGDYQGIYYYGKS
                     RKEEIENALRFSGHPKFSELENDFNQFFSDIDYYTRYGQSGMRKILFTGPPGTGKTTI
                     AKALGAKYQDKYVFVYADNYFREVCYAAANKKIPLIIIAEEADELYRADAGTLSFLDG
                     ADTPRNPAGTYVIFSTNYPRRIDPRIRKRPGRIDRVISIGAFRTKDAASCAKMYLPEG
                     ININIKELGAVLDRTTPAEIKEIINISIGMIRGTKKELSTEIIKNARAFLKGSLDLSI
                     QEAEEDIDEREDIYRQNGAQPDYLSYLED"
     gene            complement(5544..6056)
                     /locus_tag="ACHK3A_004036"
     CDS             complement(5544..6056)
                     /locus_tag="ACHK3A_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO8742055.1"
                     /translation="MKITLTIFIFLLTAFQAANAGGIPGALAGILSTLNLEIQGKVIR
                     VLDGDTIEVLQDKKPVRIRLANIDAPEKKQAFGRWSANQLKALLAAQPVTVTYTQTDR
                     YGRIIGRVFTTNGTDASRFMVQSGAAWGYERYNTDKSLPALQREAQEQKRGLWADTNP
                     VPPWEWRKNN"
     gene            complement(6135..6710)
                     /locus_tag="ACHK3A_004037"
     CDS             complement(6135..6710)
                     /locus_tag="ACHK3A_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO8742056.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKEYPVKELPDIDESAHPF
                     YVCYGGYLLNPGVSDSDISRKIKDQTGFWLAAIDDTHMDCHSIAYYDIHTLPLISCGR
                     QKIVPFAALIKADRCIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCEHR
                     FRQPVGEDNWKQAISEECAIRYANRLIQCEG"
     gene            complement(6827..7033)
                     /locus_tag="ACHK3A_004038"
     CDS             complement(6827..7033)
                     /locus_tag="ACHK3A_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742057.1"
                     /translation="MSEIRFVTLSSDTDINKIIQEGLTQKDATTTIVLGCASEGKEAP
                     LRLIETCFRTIPKHISIEEINEIK"
     gene            complement(7046..7435)
                     /locus_tag="ACHK3A_004039"
     CDS             complement(7046..7435)
                     /locus_tag="ACHK3A_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742058.1"
                     /translation="MDKSDEEKIGYILDEHERIFRKQASLYTVLGILNLIICLLCVVA
                     TFCVSVILPESHLKHLLFSFTILCMVCSFIIYYYTRHMVLNRQELLRLMRLTEGAPDV
                     KQELLNRLLSGKILTYWDEKDIRRLIF"
     gene            complement(7563..7874)
                     /locus_tag="ACHK3A_004040"
     CDS             complement(7563..7874)
                     /locus_tag="ACHK3A_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFO8742059.1"
                     /translation="MKYSVIAVIILFLLSSKSYAEEVSIDSGDPCTVFMCMAGKVYGE
                     NSSECKGPTKKFFNIIKKKKGRIRWSKTFDARKAFLMNCPGADPAHVSKIMSKFGRKL
                     F"
     gene            complement(7899..9527)
                     /locus_tag="ACHK3A_004041"
     CDS             complement(7899..9527)
                     /locus_tag="ACHK3A_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO8742060.1"
                     /translation="MGKQKIPDGWVLENGKLIQKRVTHYPPIIFGKDPYSNNLLVSYG
                     QTYLMLAAPPGSGKGVGIVIPNLLLYPHSVVVNDCKFENWDLTAGFRKACGQKVVRFS
                     PERLETHHWNPLKVINQDPLYRLGDVRTLASSLYVPDNMKNASWFSKAADVFTAIVLY
                     LIETPSLPLSLPQCYEIASLGTRIGEWAQRVIQARVGTPDALSEETVRELGVIISESK
                     GREFSTLMSFVTGRLSLYGEKLVALALTESDNEEENIDFSKLREEPTSIYFCVTEGAL
                     QKFGPLMNLFFSQAIRENSKILPEQGGNLEDGSLRLKYQVLFLEDEFAVMGRIKVMET
                     APALSRGAGLRYIIVFQNKSQVCSDDCYGPAGGKAVMDPFHIEVVFAPGDISTAEEYS
                     KRLGTYTERVASSSRNISEGQRRSRGRNVTLHARPLMLPQEVNELPYPEELVFMQPTK
                     WNPALKGRVKKIMWYKEPELTARVDPVRYPVPDIPAGNISKLKGLVVPISQYITDAVK
                     ISSPEGDYLNEEQKKRLNIPQVESERESYEDNDY"
     gene            complement(9527..9889)
                     /locus_tag="ACHK3A_004042"
     CDS             complement(9527..9889)
                     /locus_tag="ACHK3A_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742061.1"
                     /translation="MEKLKLFIVIIALCCLFFVIGLYAGGWMWLELSGVDSSPSLMTL
                     ISQGGLPALNDRARMLLPWAWCVTVAITFFPAGVSLLAMTHGADGNRKDLHGNARFAG
                     KRELRRFWYEGPEKEDRN"
     gene            complement(9901..10950)
                     /gene="virB11"
                     /locus_tag="ACHK3A_004043"
     CDS             complement(9901..10950)
                     /gene="virB11"
                     /locus_tag="ACHK3A_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFO8742062.1"
                     /translation="MNTEFDLDKSELVLGWLDQSGVTERLSRQGVTEVAINRPFEIWT
                     ETSKDGWQREDAPWLTLNMCEHLAETLAVYNNRILGVKSPIKTVELPGGERGQIVIPP
                     ACEKSTVSMTFRKPSLQRFSLENYDSSGRFDNANSVRGRSKELLDWQKELKRLNAARE
                     YVEFFRLAVRHKLNIIIFGGTGSGKTTFAKAIVDVYPSNRRLLTIEELNEMKLPYHLN
                     HVHLLYGDYVTPKDLVACCMRMKGDHILLAELTGDETWDFMELLNTGHPGTVTTAHAN
                     SSLHGYARISGLIKQSAVGAGLDISYIDRRVRTSFDVVMYMEKTEILDITYEPEVQLA
                     LLNGASMESALSFAS"
     gene            complement(11161..>12204)
                     /locus_tag="ACHK3A_004044"
     CDS             complement(11161..>12204)
                     /locus_tag="ACHK3A_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFO8742063.1"
                     /translation="FMEAHLPQDRDKTESDSRTVQSSGKQESNGPVSDKKNTQTSGGQ
                     STVNPVTPVTDIRVPETLFSPVARFDSSGISQGVGGSSDGVTSSSGYDVSAEEARLRE
                     FANADPAEIVNRALQSQADTVNGGISSDMRSNILGDLSSSRQYATAKAYKSPPRKYLL
                     KRRTNFQCVLYTAVKTDHSGFVGCRLTKPIFSADGSVILAEAGAELNGEQKIEVKQGQ
                     STVFTSWSELETTAGTEGGIRADLNGLGTGPMGSSGTDAYIDNHYGQRFGGAVMLSFI
                     QDAIATAANAAQKNKSTYSFDNSEDNAESMAEKALENSINIPPTGYVLPGTVINVIVA
                     QDIDFSSVYTVKY"
BASE COUNT         3436 a         2899 c         2021 g         3848 t
ORIGIN      
        1 tcaacaatga ttagtcctat aaatttacta cctgtaatta tccctccggt agacttcttc
       61 atatcatcat tagttttata tgggatattt gttttcattc tgtggagtac aaataaatgt
      121 tgtactccac aatttaaaaa gtttgtaaat ttgaatattc tgcttggtgt ttccttttct
      181 atcaggttat tacatcttct tttaagtttt ttactcttta tttctgtttt ttcagatatt
      241 gcaccaaaaa ttaattcagt atatccaaca acatttactt tcttcatttt taatctcctg
      301 ccgctgtgcg gctctggtga ttggggccat cccctctcga tgtattaata gtaggtcata
      361 aacgacctat ttgcaagagt tttttcttat ttttaaggaa taaaatagca ggagttcccc
      421 cctgctataa tccttaagac tgatagaaat taacattcgt ttgttcgtat ccccatgaag
      481 acacaaatat gtcaccaaca caaactgtac catttttaaa agttaacatt tttaatctcc
      541 tgccgctgtg cggctttggt gattggggac catccccttt cgatgtatta ataataggtc
      601 ataaacgacc tatttgcaag ttatttttct tcttttttgc taggcatctc gtttagaaag
      661 gacgtattca ggatgctccc cagccagtaa cagaatgtat tcaaactcta caggtgaaag
      721 ttttgatccg cttaccgttc cagtgggttc ttctttgcgc cgccatgaac gggctgtcat
      781 cccgaacatc tctgcacatt ctttctgcgt taatccaatt ttctttcgca gacgcttgat
      841 attttctggt gtattttcaa tttccatttt catctctttt tatgttttta agcataatag
      901 aaattatttc tttatcgccc tctcttaagc cgtcttgagt aaaatcaata gagccaaact
      961 cttcttcacg aaaaataatg tctgccactg tttttaccgt tgcttttgtt ttccctaaag
     1021 tcgcagactt agaaaaagtt tttacccgct tcaccagacg aaacgtatca ccttctttat
     1081 tcagataata ttgcctgtca ccttttctca ttttcatatc tatatgattc tctctaagag
     1141 aagcctcaag cggagccgaa cgactcgtac actgtacgca tggtttccct attgcaacag
     1201 atttaacata cttttccatc ttcaaactcc tgccgctgtg cggctttggt gacagagaac
     1261 tatcccttct cgatgtaacg gtaggtcata atcgacctgt ttgcaagtta ttttttcttt
     1321 ttaaatgtcg gtacaacgct tccatcacgg ttaagattca gcacggcatc aaacttagca
     1381 ccatcttttt tcttaagacc tttaatttcc cgcgtatggc ctttcatcaa caaatctgta
     1441 gcctgtgcta aggtcagttc tttaccaaac tgagtacccc acaatcggaa tccacatcgt
     1501 tcatttgaac agttataggc ttttggtgat atcttcatcc cggaaccaca taccgggcat
     1561 ctgacctttc tctcaaactc gctacgcgaa aaatcaggca cgccgcctct gtctggaaca
     1621 gtattccgac aatcaggata cttactgcat ccccaaaaaa aacctttgtc ggttctccga
     1681 cgaacataac ggccaccaca cgtacactga tactctgcta cagccaggcc agaaatatcc
     1741 acatcagaca cctgttgcaa aataaactgt tcaagttcat caagaaaatc gtcgatactg
     1801 atttcccctg tctcaatcat agattgctgc tcatgccata gagccgtcat atctggtcgc
     1861 gtcatgacct ccggtaaagc atgaataaac gataagccta actctgtagg aaccactttt
     1921 ttattcttga cagcaataaa accgcgaacc tgaagtacgt taagaatatg cccccttgtt
     1981 gccggtgttc caattccccc tcgttcctcc tctttacctt cgtctctgtc cagtaaaagt
     2041 tgtctaatcc tgggatcttc gacataacgg gccacacgcc gtagatcctc aagcaatgat
     2101 gcttctgtat ataatggtgg cggtgtggat gtctcttttc gagaattcac actactgcaa
     2161 accaattgtt gaccttttgt aaggctggtt atggcatcaa ataacttaca gtccggcaag
     2221 gtttcttcat ctctgtctgc tagtaatata ctccagccag gggaaacagg ccgtatcgct
     2281 cgttgccgga acagaaatcc attgatatta aattcaacta ccgcagaatc aaaagtttta
     2341 tcaggcataa actgcacaag atactgtgcc acaattgcct tataaaccct catttcatct
     2401 tcgttcattt gatccagccc ggtaggcgtt ggtgtcggaa taattgctgt atgagcactt
     2461 attttcttat cattaaatgc cctgcttttt cgcccactat ctatatgaga aaaaaatttt
     2521 cccagttcag gaaaaaaaga agaaagcatt gctaatgtcg ccggagactg agaaaactgt
     2581 tcttctgaga gataacggca atccgaacgg ttataagtga tagccctgta ttcctccctt
     2641 aatttttgcg ttatctccaa cactttttca gcagtgaatc catactgacg attcattttc
     2701 acctgaagat caagaagcga aaatggtaat ggtggtctcc tgcttttaat ttcactctct
     2761 gcaagagaaa cactcgcttg ttgtccctta caactacgaa ttacttcatc acaatattgc
     2821 tcgctgataa ttctaccttt atcgtccaca ggtgcatctt ccggtggaac aagcatcggt
     2881 gaaattattg tatcagtatc aataaattcg gcctgaacac accaataatc atatttctta
     2941 taatccctgt tttcttcata tcgccgaacg accagtccaa gaactggtgt ctgaacccgt
     3001 cctactgaaa gtatttcatt atgccctttt tctctggctg caatagtata tgctctggtc
     3061 atatttaaac caaaaagata atcacctgca ctacgggcaa gagctttctg ataaagcccg
     3121 taaaacgctc tgttatcctg taaattacgc atcgcctgac gcactgcatc tttattaaga
     3181 tcattaatca gaacccgttt aacgggagct ttatttccac aataagaaag tatctcttca
     3241 ataagtaact gcccttcctc atctggatcc cctgcatgaa taatcatatc tgcacgagta
     3301 attaatccag acaaaacatt aaattgtttc tctgtttttg ggataacgat atattgcaac
     3361 ggtcgtaatt gcagtggtaa tgtattttta gaccagcttt tatattcagg agttttcaat
     3421 tcaggaggta ttagctttaa caaatgacca atagcccaag aaaccgtatc attgttgcaa
     3481 actatatacc catcaaaata ctgtgggttt cccagaacct gagcaataag cttcccaagc
     3541 tctggttttt cagctatcca gagtcgcata agaatatctc cgcttgaaaa gtacgactgg
     3601 atgtatgatt taataaatca gcttccagac atttttagga attttatcaa ataattgtcc
     3661 agttacgatt tcagccaacc ggtgatctgc ggctgtcaaa aactctacaa aatcaatatc
     3721 attcattgta tatgccttac gctgaataat tttatcaaga gtagaaagct ttgagcactt
     3781 cctgagctga aacaaatact cctgcttact actttttggg tacttcctgt atttgttctc
     3841 catttaagtt attcctcaaa cggactcaat atcttcaaga ctgtcctgtt tttgatttag
     3901 tattattacc aagcatttgc atagttccct tctgatcaac aacaatctct gttgtataat
     3961 gctcctcccc atctttatct gtccattttc tgtaacgtaa tgttccctca atatagacct
     4021 gcattccttt acataaaaaa tctctggata tctctgccag tttgttataa ataacaactc
     4081 gatgccattc tgtattctct tttatttcac cagactgttt atctctccac gtttcgcttg
     4141 ttgctatact taaattagca acggtaactc cattctgcat acaccgaatc tcaggatctt
     4201 gtccaagatt acctattaat gtaactttat ttataccttt agcagccatt taatacccca
     4261 ctaatcctca agataactta aataatctgg ttgtgccccg ttctgtctgt atatatcttc
     4321 cctttcatca atatcctcct ccgcttcctg tatacttaga tcaagagatc cttttaaaaa
     4381 tgcacgagca tttttaatta tttctgttga taactctttt ttagttccgc gaatcattcc
     4441 aattgatata tttattattt ctttaatttc agcaggtgta gttctgtcaa gtaccgcgcc
     4501 aagctcttta atattgatgt ttataccttc tggtaaatac atctttgcac aactagcagc
     4561 atctttagtc ctgaaagcac caatagaaat aactctgtca attcttcccg gacgtttcct
     4621 gatacgagga tcaattctcc taggatagtt tgttgagaat attacatatg tcccggcagg
     4681 atttctcggc gtatcagccc catccagaaa acttagcgta ccagcatcag ctctataaag
     4741 ctcatcagcc tcttcagcta taataataag aggtattttc ttgttcgctg cggcataaca
     4801 aacttctcta aaatagttat cagcatatac aaatacatac ttatcctgat actttgcccc
     4861 aagagctttg gctattgtcg ttttacctgt tcctggtggg cctgtaaaca gaatcttcct
     4921 cattccagac tgaccataac gcgtatagta atcaatatca gaaaaaaact gattaaagtc
     4981 gttctccagt tcagaaaatt taggatgacc tgaaaaacgt aaggcatttt caatctcttc
     5041 ttttctagat ttaccgtaat aataaatccc ctgataatca cctattttcc agataccttt
     5101 tcttggtaac gaacgattta gtttaacgcg ccccgcttca caaagattat ggagcataaa
     5161 caaaccttta gttgttccta tagcatattc cattctaata ctggaattat gaatatatct
     5221 gacaatatgc acgacttcat ttttccccaa atgaattctg aatgctcctg acccgtcttt
     5281 aaacatgagt tttttagtcc ttcccacttt aaccattact ggattacttc ctgtatgaac
     5341 aactccagaa gaagctacag gttcgatatc aagatttgaa tatgtatttt tatttttcct
     5401 gtttataaat atttctgcta ctttaggata ataatcataa ttttttacaa tagtgccaaa
     5461 acattcatca ccataatatt ctttatgaag ccgcaaagct tgctctacca aagttaattt
     5521 ccccatatgt tacctttaat aaatcagttg tttttccgcc attcccacgg tggaaccgga
     5581 tttgtatcag cccataaacc acgcttttgt tcctgcgctt cccgttgcaa agccggtaat
     5641 gatttatctg tgttgtaacg ttcatatccc catgcagcgc cagactgaac cataaagcgg
     5701 ctggcatccg tgccgttcgt cgtgaacaca cgaccgataa tgcgtccgta gcggtctgtc
     5761 tgcgtgtacg tcacagtaac aggctgtgct gcaagcagag ctttaagctg atttgctgac
     5821 cagcgcccga aagcctgttt tttctccggt gcatcgatgt ttgctaggcg aatcctgacg
     5881 ggctttttgt cctgtaaaac ttcgatggta tcgccatcga gaacacgaat aactttcccc
     5941 tggatttcca ggtttaatgt agaaagtatt cctgctaaag caccaggtat tccacctgca
     6001 tttgcagcct gaaaggcagt aagcagaaaa ataaatattg ttaatgtaat tttcacaaaa
     6061 tactaacccc ctccggttgt tcaactccgc aaatcccaga ttaaaatcag gaatctgcgg
     6121 agtttatgat ttgcttatcc ttcacattga attaatctgt ttgcatagcg aatggcacat
     6181 tcttctgata ttgcctgttt ccagttatct tcacctacag gttgtctgaa cctgtgttca
     6241 cagccattaa aagcaaaaat cccctcgcga ttaagtatgt tggttgcaat atcccactct
     6301 ttaatcctta aaaatgccgt tactgcaaaa ccagaatatg atgcaatttt cgaaatgatg
     6361 catctgtcag cttttattaa cgcggcaaaa ggaactatct tttgacgtcc acacgaaatc
     6421 agagggagtg tgtgaatatc ataataagct attgaatggc aatccatgtg agtatcatca
     6481 atagctgcca gccagaatcc tgtctggtcc tttatttttc tgctaatatc agaatctgaa
     6541 acaccaggat ttaataaata tccaccataa cagacataga atgggtgagc agattcatca
     6601 atatcaggaa gctcttttac cggatattct ttccagtcct tcagaacacc gaactcaaga
     6661 gcatccagca aaaactttcg ttcaaatcca tcaaacaatg catattgcat atactacccc
     6721 tcacagaatg cctgatttat ataactaatg cgctatcaat agatggctgt ctcttaaata
     6781 taatgagatt cgataatacc agaataacaa cgactaccgt catcattcat tttatttcat
     6841 tgatttcttc aattgaaata tgctttggta tagtcctgaa acaagtctct atgagcctga
     6901 gcggtgcttc tttaccctca ctcgcgcaac ccaacacaat cgtagttgta gcatcttttt
     6961 gagtaagacc ttcctgaata atcttgttaa tgtcagtgtc agatgacaga gttacaaatc
     7021 tgatttcgct catatgatta cctcttcaaa aaattagacg tcggatatct ttttcatccc
     7081 aataagtcag tattttcccg gatagcagac ggttaagcag ttcctgtttc acatctggtg
     7141 cgccctctgt cagacgcata agtcgaagca gttcctgacg gttcaggacc atatggcggg
     7201 tgtaataata aataataaaa ctgcatacca tacaaaggat agtaaaggaa aataacaggt
     7261 gtttcagatg tgattccggc aatataacag aaacacagaa cgtggcaaca acacaaagaa
     7321 gacatataat taaattgagt atacccagta ccgtatataa agaagcctgt tttctgaata
     7381 tccgctcatg ttcatccaga atataaccta ttttttcctc atctgattta tccatatatt
     7441 tcattcccat agttgatgtg gaaatagtta ttcctctccg caaatctagg ataaacatat
     7501 gtcatctgcg gagaatagtt caaacgcctc tagtttagtt ctaaagcctt tgattatttt
     7561 attcaaaaaa gttttcttcc aaacttactc ataattttac tgacgtgagc aggatctgcc
     7621 ccagggcaat tcattaaaaa tgcttttctc gcatcaaaag ttttactcca ccgaatacgc
     7681 cctttcttct tttttataat attaaagaat ttttttgttg gtcccttaca ttcagatgaa
     7741 ttttccccat aaacttttcc agccatacac ataaagacag tacagggatc ccccgaatct
     7801 atacttacct cttcagcata gcttttactg gataacagga aaagaataat caccgctatg
     7861 acagaatatt tcatattaac ccccttttta ttaaaaattc agtaatcatt atcttcataa
     7921 gattcccttt ctgattccac ttgtgggatg tttaatcgtt ttttctgctc ctcatttaaa
     7981 taatcacctt ctggtgaaga tatcttaact gcatccgtaa tatactgact tattggtaca
     8041 acaagtcctt taagtttcga tatattcccg gctggtatat caggcacggg ataacgaaca
     8101 ggatccactc ttgctgttaa ttcaggttcc ttgtaccaca ttattttttt cacccggcct
     8161 ttcaaggcgg gattccattt tgttggctgc ataaacacca gttcttcagg atatggaagt
     8221 tcattaacct cctgtggcaa cattaaaggc ctagcatgca gtgtaacatt acgtcctcga
     8281 gaacgacgct gtccttccga tatattcctg ctactggacg caactcgttc tgtgtacgtt
     8341 cccagtcgtt tactgtactc ctccgctgta ctgatatctc cgggggcaaa tacaacctca
     8401 atatggaatg gatccattac agctttcccc cctgcgggtc cataacagtc atcagagcaa
     8461 acctgacttt tattctgaaa aacaattata tagcgtaatc ctgcccccct gcttagtgcc
     8521 ggggctgttt ccataacctt gatacggccc atcacagcaa attcatcttc cagaaaaagc
     8581 acctgatatt tcaatctgag agaaccgtcc tccagatttc ccccttgctc cggtagtatt
     8641 ttactgtttt cccttattgc ctgagaaaaa aacaaattca ttagtggacc aaatttctgt
     8701 aaagctccct cggtaacaca aaaataaatg ctggttggtt cttcccttaa cttgctgaag
     8761 tcaatatttt cttcctcgtt atcactctcc gtcagcgcca gcgccaccag tttttcgccg
     8821 tacaagctta gacgtcccgt gacgaatgac atcaacgttg aaaattccct gcctttcgac
     8881 tcggaaataa tcacccccaa ctctcggacg gtttcttctg acagtgcatc tggcgttcct
     8941 acacgagcct gaataacacg ctgcgcccat tctccaatac gagttcccaa cgatgcaatt
     9001 tcgtaacatt gcggtaaact caatggcaac gatggtgttt cgatcaggta taaaactatc
     9061 gcggtaaaca catctgcggc tttagaaaac caactggcat tcttcatgtt atcaggtaca
     9121 tacagagatg aggctagtgt tcgaacatct cccaagcgat acagcggatc ctgatttatt
     9181 acctttaacg gattccagtg atgggtttcc agtcgctctg gcgaaaaacg cacgactttc
     9241 tgaccacatg cttttctgaa acccgctgtc aaatcccagt tttcaaattt acagtcattc
     9301 actacaacag aatgcggata caatagtaga ttagggatca caatccccac ccctttacca
     9361 cttcccggcg gagcggccaa catcagatag gtctgtccat aggatacaag taaattatta
     9421 ctgtacggat ctttaccaaa aataattggc ggataatgag ttactctttt ttggattaac
     9481 tttccatttt ccagcaccca tccgtctggt attttttgtt ttcccattaa tttctgtcct
     9541 ccttctctgg cccttcatac cagaaacgac gtaattcccg ttttccggca aacctagcat
     9601 taccatgtag gtcctttctg ttgccgtccg ctccatgagt catggccaaa agagaaacac
     9661 ctgccgggaa aaaggtaatt gcaactgtca cacaccacgc ccagggaagt aacattctgg
     9721 cccgatcatt cagtgctggc agtcctccct gacttatcag ggtcatcaaa gatggagagg
     9781 agtcaacacc tgataattca agccacatcc atcctccagc gtaaagccca ataacaaaaa
     9841 acaaacaaca aagagctatt atcacgataa atagtttgag tttttccata cagcactacc
     9901 tcacgatgca aaagaaagag cactttccat gctggcacca ttcagtaatg ccagttgcac
     9961 ctctggctca tacgtaatat ccaagatttc tgttttctcc atatacatca caacatcgaa
    10021 agacgttctg acacgtcgat cgatataaga gatatccagc ccggcaccaa ccgcagactg
    10081 ctttattagt ccagagatac gagcataacc atgtaaactg ctattagcat gcgctgtcgt
    10141 aaccgttcct ggatgcccgg tattcagcag ttccataaaa tcccaagttt catcgcctgt
    10201 cagttctgcc agaagaatat gatccccctt catgcgcata caacacgcta ccaggtcttt
    10261 tggtgtcaca taatctccat acaataaatg tacgtgatta agatgataag gcagcttcat
    10321 ttcattcaat tcttcaatag tcagtaaccg acgatttgac gggtaaacat caacaattgc
    10381 tttagcaaaa gttgtttttc ccgaaccggt tccaccaaaa ataataatat ttagtttatg
    10441 tctcacagcc agacggaaaa attcaacata ttcacgagca gcattaagac gttttaattc
    10501 cttttgccag tccagtaact ctttacttct tcctcttaca ctattggcat tatcgaaacg
    10561 tccactggag tcataattct cgagagagaa acgttgaaga gacggtttac gaaacgtcat
    10621 cgacacagta gatttctcac aggcgggagg aataacaatt tgtcctcgtt cgcctcctgg
    10681 tagttccact gtttttatcg gtgacttaac acctaatata cggttattat aaacagccag
    10741 tgtttcagcc aggtgttcac acatattcag cgttaaccac ggtgcatctt ccctttgcca
    10801 gccatctttt gatgtctcgg tccagatctc aaaaggtctg ttaatcgcta cctccgtcac
    10861 tccctgacgg gacagacgtt ctgtaacacc agactgatcc agccacccca aaacaagttc
    10921 tgacttatcg aggtcaaatt cggtattcat taatagacgc cctttttatt tacactttta
    10981 tgagttactt ttctatctca ttaatgaaat gataaatgca gcaacgatca ttatgattga
    11041 ccaccgtaaa tttattggga tacgataaaa ataaatacac acccatttaa aaaatcccat
    11101 ccaacctcct tcaagcaatt actttaatat atagccaccc gttaaggcgg ctatatatca
    11161 ttaatattta actgtataga ctgacgaaaa atcaatatcc tgagcaacta ttacattaat
    11221 aactgttcca ggtaatacat atcctgtagg tggtatattt atagaatttt ccagtgcttt
    11281 ttcagccata ctttccgcat tatcctcact gttatcaaaa ctatacgttg atttattttt
    11341 ttgtgccgcg tttgcagctg ttgctatcgc gtcctgaata aacgatagca tcacggctcc
    11401 accaaacctc tgcccataat gattatcaat gtatgcatcc gtgccactgc ttcccatcgg
    11461 ccccgtccct aatccattta aatctgctcg tataccccct tctgtcccgg cagtagtttc
    11521 aagctcactc catgaagtga acaccgtaga ttgcccttgt tttacttcaa ttttttgttc
    11581 cccattcaat tcagcacctg cttcagccaa aataacacta ccgtccgctg aaaaaatggg
    11641 ttttgttaac cgacaaccaa cgaatccaga gtgatccgtt ttcaccgccg tataaagtac
    11701 gcactgaaaa tttgttctcc ttttcaggag atacttacgt ggaggcgatt tataagcttt
    11761 agctgttgca tactggcggg atgatgagag atcccccagg atgttagatc tcatatccga
    11821 agatattccc ccattcacag tatctgcctg actctgtaaa gcacggttaa ctatctctgc
    11881 cggatctgca ttggcaaact ctcgtagacg agcctcttct gccgaaacat cataaccaga
    11941 agatgatgtt acaccgtcac tactaccacc aactccttgg ctaatgccgg aagaatcaaa
    12001 tcgtgcaaca ggcgaaaaca acgtttctgg tactctgata tccgtaaccg gtgtaaccgg
    12061 gttgaccgta gactggcccc ctgaagtttg agtatttttt ttgtctgaaa caggtccgtt
    12121 actctcctgc tttcctgacg actggacggt tctactgtca gattccgttt tgtctctgtc
    12181 ttgtggtaaa tgagcctcca taaa
//
LOCUS       DAZGHF010000137        12016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGHF010000137 DAZGHF010000000
VERSION     DAZGHF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.138"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            98..1330
                     /gene="dgcM"
                     /locus_tag="ACHK3A_004045"
     CDS             98..1330
                     /gene="dgcM"
                     /locus_tag="ACHK3A_004045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO8742064.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(1351..1914)
                     /gene="smrA"
                     /locus_tag="ACHK3A_004046"
     CDS             complement(1351..1914)
                     /gene="smrA"
                     /locus_tag="ACHK3A_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO8742065.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLTQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(2109..3623)
                     /locus_tag="ACHK3A_004047"
     CDS             complement(2109..3623)
                     /locus_tag="ACHK3A_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFO8742066.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGSAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VVIFSTVYLILVFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
     gene            complement(3620..4648)
                     /locus_tag="ACHK3A_004048"
     CDS             complement(3620..4648)
                     /locus_tag="ACHK3A_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8742067.1"
                     /translation="MIISKKQLIGVVAIGIILAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSRKANLES
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLVQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            4745..5560
                     /locus_tag="ACHK3A_004049"
     CDS             4745..5560
                     /locus_tag="ACHK3A_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8742068.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            complement(5734..5950)
                     /locus_tag="ACHK3A_004050"
                     /pseudo
     CDS             complement(5734..5950)
                     /locus_tag="ACHK3A_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            5950..6093
                     /locus_tag="ACHK3A_004051"
                     /pseudo
     CDS             5950..6093
                     /locus_tag="ACHK3A_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            6104..6619
                     /gene="ogt"
                     /locus_tag="ACHK3A_004052"
     CDS             6104..6619
                     /gene="ogt"
                     /locus_tag="ACHK3A_004052"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO8742069.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISAANPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            6814..7566
                     /gene="fnr"
                     /locus_tag="ACHK3A_004053"
     CDS             6814..7566
                     /gene="fnr"
                     /locus_tag="ACHK3A_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO8742070.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            7718..8668
                     /gene="uspE"
                     /locus_tag="ACHK3A_004054"
     CDS             7718..8668
                     /gene="uspE"
                     /locus_tag="ACHK3A_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO8742071.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(8718..8975)
                     /gene="ynaJ"
                     /locus_tag="ACHK3A_004055"
     CDS             complement(8718..8975)
                     /gene="ynaJ"
                     /locus_tag="ACHK3A_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO8742072.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            9219..10250
                     /gene="ynaI"
                     /locus_tag="ACHK3A_004056"
     CDS             9219..10250
                     /gene="ynaI"
                     /locus_tag="ACHK3A_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO8742073.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            complement(10301..11914)
                     /gene="mppA"
                     /locus_tag="ACHK3A_004057"
     CDS             complement(10301..11914)
                     /gene="mppA"
                     /locus_tag="ACHK3A_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO8742074.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAQTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPVYDKVLAQASTENTVKARNADYNTAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT         3243 a         2897 c         2677 g         3199 t
ORIGIN      
        1 taaatagcgt ctatcattat cagaattatc tgatcatatg acgtggcttt tttgcgatcg
       61 gatagcaaca aaaattgata aaaataacgg gatctcaatg attacgcaca acttcaatac
      121 cctggactta ctcaccagtc ctgtctggat cgtttcgccc tttgaggaac agttaattta
      181 tgccaacagc gcggcgcgac tgttgatgca agacctcacg tttagtcagc tacgaaccgg
      241 tccctattcc gtctcctcac aaaaagaact gccgaaatac ctctccgatc tgcaaaacca
      301 acacgatatt atcgaaatcc tcaccgttca gcgcaatgaa gaggaaacag cattgagctg
      361 tcggcttgtt ttgcgaaagc tgacagaagc agaaccggtg attattttcg aaggtatcga
      421 agcaccggca acgctgggtt taaaagccag tcgctcggca aattatcagc gcaaaaaaca
      481 aggtttttat gcgcgctttt ttctgactaa ctctgcacca atgttgttga ttgacccctc
      541 acgagatggc caaatcgtcg atgctaacct cgccgcgctc aatttctatg gctataacca
      601 tgaaacgatg tgccagaaac atacctggga aataaatatg ctcgggcgac gcgtcatgcc
      661 tatcatgcat gaaatttcgc atttacccgg tggccataag cctttgaatt ttgttcataa
      721 actggcggat ggttcgactc gtcatgtgca gacctatgcc ggtccaattg aaatttatgg
      781 cgacaagctc atgttatgta ttgtgcatga tattactgag caaaaacggc tggaggagca
      841 gctggaacat gctgcccacc atgatgcgat gaccggatta ctgaatcggc gacagtttta
      901 tcacattacc gagccaggcc aaatgcagca cctcgccatc gctcaggatt acagcttgtt
      961 gctcatcgac accgatcgtt ttaaacacat taacgatctc tatgggcatt ctaaaggtga
     1021 tgaggtgtta tgcgcccttg cccgcactct cgaaagttgc gctcgcaaag gcgatttggt
     1081 gtttcgttgg ggaggcgaag agtttgtctt attgctacca agaaccccac tggataccgc
     1141 gctttcgctg gctgaaacta tccgcgtaag cgtggcaaaa gtgagtattt cgggcttacc
     1201 acgctttact gtcagcattg gtgtggcgca ccacgaagga aatgaaagca tcgatgaact
     1261 gtttaaacgc gttgatgatg ctttgtatcg agcgaaaaat gatggacgca accgcgtgct
     1321 ggcggcataa gccacggatg cgtctcgtga tcaacgactg cgcttagcgt ggcgctccca
     1381 gttttcttgc ttcgcctgcg ctgttttacg tagtgcgacg taacacgccc cgctgccgcc
     1441 atgatgcggt agcgcagtgc aatatgcctg aacatcatca aattcggtca gccagcgcgc
     1501 cacatagctg cggacaatat tggcatgcga tttatcatcc cgtcctttgc catgaataat
     1561 cagcacgtta cgcaaaccat ccgccagggc ttgttgaatg aaactgaaca ccattttgcg
     1621 gcactcttcc accggctggc gcaaaagatt caggctcgcc tgttgcggat atttaccact
     1681 gcgcagctta tccagcaccc catgttgcaa cccttcccgc cgaaactcca gcggctgagt
     1741 tagcgggatg atgtcgagaa atccggtggt gaggaaatta tcaagctgca gcgtgtcgat
     1801 acgctgcggc gcacgttggt tacgcgttgg atgccagtgg acatctgtag cacgtttcag
     1861 cggctggaca tcttccatgg cgtcaagaaa cagcgatttg tcgtcaaggt tcatgtaacc
     1921 tcctatgcac cttcacacgt cgtgattcag acgggttaat tgttcatact tactgcatcg
     1981 ttcccttcac agattgaaac aatactctac ttcatacctg tgagtatctg cgcaggcggg
     2041 gcattttata ctagcctgtt agcagtatcg aacagtttag cccgagttta attcagcaca
     2101 ggctccgatc aggatgattt attcatagca ggaaccaaca aaaatgcgat aaataacaga
     2161 ccactgatta gcagaaaggt atccgaaaat gccatagttt gcgcctccct cagcatcaac
     2221 tgactcaata atttggttga agccagtatt tccgatgtct ggtcacttcc actgcgatta
     2281 aaaaacaaag cacttctgga aatgaagtca ttcaccgtat ggggaacaga aaccattttt
     2341 tcacccattc tcgaaaaatg aaaattagtt cgattattga gaatagagcc acacagggca
     2401 ataccaatcg cgcccccaag attacgtgtt aaattaaata caccagaggc taacttcagc
     2461 cgttcctttg gaattccccc cagcgttaat gttacgatgg gggccatcgc aaactgctgc
     2521 gaaatccctc taattgcctg tggaaacagt aactcctgcc atccccattc gtgggttata
     2581 ggggtaaaaa gatacattga aaacacaaat ccacccagcc ccgccatcaa cagccatcgc
     2641 agatttattt ttttacttaa ccagaagtaa aagggaacag aaaataactg gaaaatacct
     2701 gttgtgcaaa cggcaaaacc tatctcttcg gcatttaatc cgcgaacctg ccccaaaaac
     2761 acaagtatta aatatacagt agagaatatc acaacccctc cagaaaaaga aaaaaaacat
     2821 cccagcgtga aatttttatc cttaaaagca tgaagatcca ttatcggatt ggatattgta
     2881 agtgtacgcg cagcaaataa aataaaactc accaatgcca gaactgatgt cagcaaaata
     2941 gtattatcat ccagccatcc ccagcgcgct ccttcttcaa gggtatattc cagacatccc
     3001 agtgtcatgg ccaacaggat aatactcgga taatcagcaa ctttcagtaa agagagatca
     3061 ggtttatcaa aattaaccag aaaaggaata ctcaaaacta aatatatgcc aggtaatata
     3121 ttgatataaa atagccatcg ccagtcgagg ttatcagtga tccagccccc cagagttggc
     3181 cctagcgtcg gtgacagtga ggccagcgca ctgactaccg cagcagccga ccccaactct
     3241 ttaccctggt aataaataaa cgccgtcgtg aaaactaaag ggatcatgga tgcgccagcc
     3301 gctccctgta aagcacgaaa caaaatcatg atctgaatat tccatgcaag tccacacgcg
     3361 atactcatta aagtaaatat tcccgcggat agagtaaaaa gccaacgagt ggaaaatacc
     3421 cgtgacagcc acccagagag cggaataaca ataatctcag caattaaata gctggtctga
     3481 agccaggcca tttcgtcttt acctgcggac agaccaccac ctatttcatt tagggatgac
     3541 gatacaattt gaatatcaag ctgagcaata aataatccca tacacatact ggcgaaagca
     3601 atatgtttac gcatagacat catctctcat cgacactgac ggtgacggac agtcccgggc
     3661 gaagaagttg ctttaattcc tttgcgtcgt caaatgcaat ccgtaccggg acacgctgaa
     3721 caattttggt gaaattgcca gtggcatttt ctatcggaat caagctgaaa ctggctcctg
     3781 tcgctgggga aaggctttca atgtgaccgt gaaacacttc ccctttgaga atatcagcac
     3841 ggatctcagc cttcattcct ggcttcattc ccgctatttg gttttcttta taatttgcat
     3901 caacccacaa ctcactgact ggtacaagtg aaactaacga tgtgccgcct tccacccaac
     3961 tgcctgtatg ggcgctgcgg tttgcgacaa tgccatcaat cggcgcacga acaagggtat
     4021 attccagatc cagtagtgcc tgagtcaaag cggcctgaac ttcttcgata ctcgcacgta
     4081 cgttttcttc ctgagcagat aaaactgcca gccgttgctt ctccaccagg aaatcgttcg
     4141 cggctttgcg ctcctttgct gcgacctgct ggtagtcaaa cctggcgtta tcaataattt
     4201 gctgcgatat tgccgctgat tgtacgagtt tttcataacg ttcagtatct cgcaggcgct
     4261 tttgctcctc atgttttact gcctgccatg tttcactggc actttgaatt atcgactgct
     4321 gcatcgtaat agtggcttga atgctttcga gattggcttt tctacttttt atttttgcct
     4381 caagcctggc gacatttgca cgataatccc gatcatcgag acgcaggata atatcgcctt
     4441 tttttacgct ctgattatca cgcacctcta tagcagaaat ataaccagaa actttacttg
     4501 ccaccgtggt aatattacca ccaatgtaag catcatccgt cgtctgtata aatcttcctt
     4561 tgcttaccca ccagataaaa aagacaacgc cagccaaaat aataccgatc gctactaccc
     4621 caataagctg ttttttagaa attatcatca ctctttacac cgtatatatc cggaaacttg
     4681 cattcgccat atttaaaatt taacatggct tatttgatga tttctgacaa aatacctact
     4741 gtaaatgaca atcacactgc aacccaaagc atcaccaggg catcacatta tcggactgga
     4801 ttcggaacat ctgaatggag gcacagttcc ctggcataaa catctctatg cccagttgct
     4861 ttacccggct gaaggagtcg ttcgtgtatg ggctggagaa agcgtctggc tagtgcatgc
     4921 cagcagcgcg ctttggctgc ccccacaaat gccgcataag tttgttgcga caggcaacgt
     4981 acttttaaaa accgtactgg tttcagaagc ggaaagtgaa acgctgggaa aagtttgctt
     5041 catgacgggt atttcacccc tgttaagaga attactaatt gcaattaacc aacttccacc
     5101 gtcgcaaagt accacagata aacaacagtt acgtttttca gccctcgaga cactgatact
     5161 tcaggaaata aaaatggggg tgaaaatgtc acttgaactc ccttggccaa atgatgagcg
     5221 gctacagcag ctttgtgaga atttgctaaa caaccaaggg tatctgccaa cactggataa
     5281 tctagcggat aaaattaacg tcagtagccg tacactcatg cgcttatttg ttaaagagac
     5341 gggattaaca tttcgccact gggttcagca aatgcacgtt atttctgcag tgacgcttct
     5401 tgatgatgga tattcgttga ccaaaattgc ccatcgtctg ggctatgcct cagctgaatc
     5461 gttcggcaat atgtttaagc gtcgaacggg ctactcgccc ggtaaattta ctcgtcgatt
     5521 aacgatgcat gattatgcaa tcacaaggca aatgatttaa cttatatttt cgtgaaatct
     5581 gtcactgaag aaaattggca acggatgggt aaaaccgtta taacactgtc accggtcacc
     5641 aggaccccag gccggattta agacgaggat gcactgctgt gtgtactgta gagtctggcg
     5701 gatgtcgaca ggctctattt ttttattcat tttttagctc tgtaaataac cacattctcg
     5761 ccgggattgg ggaatgagtg acgagatcag tggtgtctgg cggctatcac gccacagaat
     5821 caaataacag atagcctacg gtaaaatttc gctgactgga attatcccac cctcgatcca
     5881 tgtgatggct tgccctcatt tcttcaggca gaaaaatcaa aaaatcgctt tatgctatca
     5941 gactgcgaca aaccagacgc gaaactgggt acttactatt cgttagtctt gccctatccg
     6001 cttatctttt tggtggtatg gctgctgatg ttgctggcgt ggtatcttgt tggtctgccg
     6061 ataggtccgg ggatttaccc acgtttgtct taagagagaa cggatgctga gattacttga
     6121 agaaaaaatt gccacgccac tgggtccact atgggtgatt tgcgatgagc aatttcgcct
     6181 gcgggcggtt gaatgggaag agtacagcga acgcatggtg cagctgctgg acatccatta
     6241 tcgcaaagaa ggctatgagc gcatttctgc cgccaaccca ggtggtttaa gcgacaagct
     6301 tcgtgaatat tttgccggta atcttagcat tattgatacg cttcccactg ccacgggggg
     6361 gacgccattt cagcgcgaag tctggaaaac actacgcact atcccctgcg ggcaggtaat
     6421 gcattacggt caactggccg aacaattggg tcgccctggc gcggcgcgtg ccgttggtgc
     6481 ggctaacgga tcgaatccca tcagcattgt tgtaccttgc catcgggtta ttggccgaaa
     6541 cggcaccatg accggatatg caggcggagt tcagcgaaaa gagtggttat tgcgccacga
     6601 aggttatctt ttgctgtaaa cattaaacaa tttgtgccag cttgttcaca cttttatgta
     6661 aagttaccct taacaactta agggttttca aatagataga catatattta catctaatat
     6721 cggaattctc tgctgttaag gtttgcttag acttacttgc tccctaaaaa gatgttaaaa
     6781 ttgacaaata tcaattacgg cttgagcaga cctatgatcc cggaaaagcg aattatacgg
     6841 cgcattcagt ctggcggttg tgctatccat tgccaggatt gcagcatcag ccagctttgc
     6901 atcccgttca cactcaacga acatgagctt gatcagcttg ataatatcat tgagcggaag
     6961 aagcctattc agaaaggcca gacgctgttt aaggctggtg atgaacttaa atcgctttat
     7021 gccatccgct ccggtacgat taaaagttat accatcactg agcaaggcga cgagcaaatc
     7081 actggtttcc atttagcagg cgacctggtg ggatttgacg ccatcggcag cggccatcac
     7141 ccgagcttcg cgcaggcgct ggaaacctcg atggtatgtg aaatcccgtt cgaaacgctg
     7201 gacgatttgt ccggtaaaat gccgaatctg cgtcagcaga tgatgcgtct gatgagcggt
     7261 gaaatcaaag gcgatcagga catgatcctg ctgttgtcga agaaaaatgc cgaggaacgt
     7321 ctggctgcat tcatctacaa cctgtcccgt cgttttgccc aacgcggctt ctcccctcgt
     7381 gaattccgcc tgacgatgac tcgtggcgat attggtaact atctgggcct gacggtagaa
     7441 accatcagcc gtctgctggg tcgcttccag aaaagcggta tgctggcagt caaaggtaaa
     7501 tacatcacca tcgaaaataa cgatgcgctg gcccagcttg ctggtcatac gcgtaacgtt
     7561 gcctgatttt tccgcataac tcactatcct tctgtcatat cattaaattt ttctgattta
     7621 ttgatctggc agaaggttca tcactgtttc attcaccaga tatgggttaa tcttttaatt
     7681 acaaactgcg ttgacagttg ttgtaaggag accctgtatg gctatgtatc agaacatgct
     7741 cgttgttatc gatcctaacc aggacgacca accagcattg cggcgagctg tttatttaca
     7801 tcaacggatt ggtggcaaaa ttaaagcctt tttgccgatc tatgacttct catacgaaat
     7861 gaccaccctg ctctccccgg acgaacgtac cgctatgcgt cagggcgtca tcagccagcg
     7921 tacagcctgg atccacgagc aggcaaaata ttatctcaat gctggcgttc ccattgaaat
     7981 taaagtggtc tggcataacc gtcctttcga agccatcatt caggaagtga tcagcggcgg
     8041 gcacgatttg gtgctaaaaa tggcgcacca acatgaccgt ctggaagcgg tgatttttac
     8101 gccaacggac tggcatctgt tacgcaaatg cccaagcccc gtgtggatgg tgaaagacca
     8161 gccgtggccg gaagggggta aggcgctggt ggcggtgaat ctcgccagtg aagagccgta
     8221 tcataatgcg ctcaatgaaa aactggtcaa agagacgatc gaactggcgg aacaagtcaa
     8281 ccataccgaa gttcatctgg ttggcgctta tccggtaacg ccaattaata tcgcgattga
     8341 actgccggaa tttgatccga gcgtttataa cgatgccatt cgtgggcaac atttgctggc
     8401 aatgaaagcc ctgcggcaga aattcggcat taatgaaaac atgacgcacg tagaaaaagg
     8461 tctgccagaa gaggtgattc ctgatttggc ggagcactta caggcgggga ttgtggttct
     8521 gggcacggtc ggacgcaccg gtatttcagc agcattcctt ggtaacacgg cggaacaggt
     8581 gattgatcat cttcgctgcg acctgctggt cattaaacct gaccagtatc agacacccgt
     8641 tgaactggat gacgaagaag acgattaacg actcttcaat aactgacaag gcccgagtta
     8701 tcgggcctat tttttactta ctgctcttcg ccgccaagga aataaattcc caacgggatt
     8761 gccagcaaca cagtaaagac caggctataa acaaaaatca tcgatgactg aatcacatac
     8821 attgatgtcg tccactcaga cagcggaata ttgtactgtt ctatcacgcc gccgatggtc
     8881 gcgcgagtca caaccgacgc cgcaataatg aaaaccgcca acaaaccaaa gagaaatttc
     8941 ttccctttcg ctgacttcag tttcgccata atcatgatgt aatccttgtg cagacaaatt
     9001 tcttgttacc taaacgtaac aataccatga catttcaccg ttgtctgctc tgaaaacctc
     9061 acctcttagc tggatttaca atcaacgcat tgattagtta gctaatgttg ctacttcctt
     9121 ccctgactat tgatgatctg aatgaattgt cgcggtaaaa tagccccgcc aggggtttcc
     9181 ggcatgacga caattatctg gaggagcctg ataaaacaat gatcgctgaa ctgtttacaa
     9241 ataatgcgct taatctggtc attattttcg gtagctgcgc agcattgatt ctgatgagct
     9301 tttggtttcg ccgcggaaat cgtaaaagaa aaggattttt attccatgcg gtgcaatttt
     9361 taatctacac cataattatc agtgctgttg gtagcatcat taattatgtt atagaaaact
     9421 ataaactcaa atttatcacc ccaggcgtta tcgattttat ctgtacgtcc ctgattgcgg
     9481 ttattctgac gattaagcta tttctgctga taaatcagtt tgaaaaacag cagattaaaa
     9541 aaggtcgcga tatcaccagt gcgcggatta tgtcgcgtat catcaaaatc accattattg
     9601 tggtgcttgt tctgctttat ggtgaacatt tcggtatgag cctttctggc ttgttgacct
     9661 ttggtggtat tggtggtctg gctgtcggta tggccggtaa agatattttg agtaacttct
     9721 tttccgggat tatgctctat ttcgaccgtc cttttagtat tggcgactgg atccgttcac
     9781 cggacagaaa tatcgaaggt acagtagcgg aaattggctg gcgaattacc aaaattacga
     9841 cttttgataa tcgtccgttg tacgtaccga actcgctgtt ttcgtcgatc agcgtagaaa
     9901 acccaggacg aatgaccaac cggcgcatta ccacgaccat tggtttacgt tatgaggatg
     9961 cggcaaaagt gggcgttatt gtcgaagccg tacgtgagat gctgaaaaat cacccggcca
    10021 tcgaccagcg acaaacctta ctggtttatt tcaaccagtt tgctgactct tcattgaata
    10081 ttatggttta ttgctttacc aaaaccacgg tatgggctga atggcttgct gcacagcaag
    10141 acgtttattt gaagattatc gatattgtac agtcacacgg cgcggacttt gccttcccca
    10201 gccagacgct gtatatggat aacattacac cgccggacca gggtcgctaa atcagacatc
    10261 actggcgtta tccacaaggg taacgccagt tcccatcaca tcaatgcttc acaatataca
    10321 tagtccgact gtacgccaca tcttccgggt tattaatggg ataacctttc agccacggct
    10381 tgattaatcg tccattggta tattgataaa ttggtgcaat cggtgcttgc tccatgagga
    10441 ttttttctgc cgtattgtaa tcggcattac gcgctttaac ggtattttcc gttgatgcct
    10501 gggccagtac tttgtcgtat accggattgt taaagcgtga aatatttcct gaatgcgttg
    10561 acgttaataa tgtcaggaaa gtggagggtt cattataatc ccctacccac gaggctcgga
    10621 taacatcaaa attgccggtg ttacggctat cgatataggt tttccattcc tgattttgca
    10681 atttaacatc tacgccaagg ttctttttcc acattgatgc tacagcaatc gcaatttttt
    10741 gatggttttc tgaagtgtta tacaaaagcg tcagcttcag cggtttttgc ggaccataac
    10801 cagctgcgct caacaaagtt tttgcctgcg cattcagttc ttcctgactc atttgttcaa
    10861 acggcgaagg ttccggcgta aatcctgcag taacatctgg tgtaaaatgc cacgctggct
    10921 tttcgcccgt tcccaacact ttttcggtca tcaggcggcg atctatcgtc atacttaatg
    10981 ccaggcgaac acgctgatcg gccgtcggcc ctttttgcgt gttaaacgca taataatagg
    11041 tcccgagctg tggcggcgta taaacctgcc ccggaatatc cttcaacagc ttctgataca
    11101 tatttttcgg gaaggattcg gtgatatcaa tatcccccgc gagataacgc ttagtggctg
    11161 cggattcctg attaattggc aggaaggtca ctttttgcag caccgttttg gcgttatccc
    11221 aataatgggt attcggtacc acgaccagtt tttcattgac tacgcgctct ttaagaacat
    11281 aagcgccatt accgatcaga tttccgggtt tcgtccactc tttactgctt tctacgttgg
    11341 ctttttgcac cgggaagaag gcaaagttag cggttaaatt cacaaaccac ggcaacggtt
    11401 tatcaagctg gattttcaaa gtttgggcat caactgcggt gacgccaagc tggtcaggcg
    11461 cggctttacc atcaataatc gcctgtgcgt tgttgattcc cgccagcgcg gcaaaccatg
    11521 caaatggcga caatgttttt gggtccacca gacgttgcca gctgtagaca aaatcttgcg
    11581 ccgttaccgg tgtgccatcc gcccattttg cgttatcgcg cagggtaaaa gtccagatac
    11641 ggttgtcatt acttttccac tgagtcgcaa cgccggggac aatctcccct ttctcgttct
    11701 gattcaccag tccttcaaac agatcgcgaa tgacctgaat ctctggcagg cccacggctt
    11761 tagcgggatc cagcgacgca ggctcatctt taatgtggcg caccagctcc tgcttctctg
    11821 ccagtactgt gccgctcgga acttcagcag catacgaaag agaaatgctg ctgaccaaca
    11881 gcgcacaaca cgtgactgaa acagagtgct tcataagttt ccctctaacc gacattaaaa
    11941 ttgatgcgta attatttgtt tcgccttaaa gaaatgcaaa caacttccat caaaaaagtg
    12001 acaaaggaag catcaa
//
LOCUS       DAZGHF010000138        11955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGHF010000138 DAZGHF010000000
VERSION     DAZGHF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.139"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            35..568
                     /locus_tag="ACHK3A_004058"
     CDS             35..568
                     /locus_tag="ACHK3A_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8742075.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKKKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            842..1537
                     /locus_tag="ACHK3A_004059"
     CDS             842..1537
                     /locus_tag="ACHK3A_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO8742076.1"
                     /translation="MKEMISVDHEISMSSLDFLNNIINPIRVEAGKPPHEPRKFLAKI
                     EDELELEVTWKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPRTLPEALRFAAKLAEQKAVLENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            1632..1763
                     /locus_tag="ACHK3A_004060"
     CDS             1632..1763
                     /locus_tag="ACHK3A_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742077.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVVLFLSITWMALTSAGLVG"
     gene            1986..2192
                     /locus_tag="ACHK3A_004061"
     CDS             1986..2192
                     /locus_tag="ACHK3A_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO8742078.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            2257..2481
                     /locus_tag="ACHK3A_004062"
     CDS             2257..2481
                     /locus_tag="ACHK3A_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742079.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            2826..3152
                     /locus_tag="ACHK3A_004063"
     CDS             2826..3152
                     /locus_tag="ACHK3A_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO8742080.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(3284..3484)
                     /locus_tag="ACHK3A_004064"
     CDS             complement(3284..3484)
                     /locus_tag="ACHK3A_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8742081.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            3526..3891
                     /locus_tag="ACHK3A_004065"
     CDS             3526..3891
                     /locus_tag="ACHK3A_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO8742082.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            4175..4732
                     /locus_tag="ACHK3A_004066"
     CDS             4175..4732
                     /locus_tag="ACHK3A_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO8742083.1"
                     /translation="MLTTQKRKFALALMSGKNRTAAAIEAGYSEKSARCKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQACHREVPPVEKKNQVSVPPDDGQSFSPLSG
                     IDYMEDGLPDPVKAMGRILVENISIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGDAA
                     KKAANRFAVPPPPRLVVNNDNEGNG"
     gene            4729..6390
                     /locus_tag="ACHK3A_004067"
     CDS             4729..6390
                     /locus_tag="ACHK3A_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO8742084.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVASGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            6454..8391
                     /locus_tag="ACHK3A_004068"
     CDS             6454..8391
                     /locus_tag="ACHK3A_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO8742085.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGVHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            8436..8657
                     /locus_tag="ACHK3A_004069"
     CDS             8436..8657
                     /locus_tag="ACHK3A_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742086.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            8603..11025
                     /locus_tag="ACHK3A_004070"
                     /pseudo
     CDS             8603..11025
                     /locus_tag="ACHK3A_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            11022..11348
                     /locus_tag="ACHK3A_004071"
     CDS             11022..11348
                     /locus_tag="ACHK3A_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO8742087.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            11358..11708
                     /locus_tag="ACHK3A_004072"
     CDS             11358..11708
                     /locus_tag="ACHK3A_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO8742088.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            11705..>11955
                     /locus_tag="ACHK3A_004073"
     CDS             11705..>11955
                     /locus_tag="ACHK3A_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFO8742089.1"
                     /translation="MIETLLDFSGLEEISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VASRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNP"
BASE COUNT         2739 a         2828 c         3700 g         2688 t
ORIGIN      
        1 ggtattcgga accgataaat cctgataaat atccatgaac gcaaaaatca gatacggcct
       61 gtcggctgcc gttctggcgc tgattgccgc aggggcgcct gcgcctgata ttctcgacca
      121 gtttctggat gaaaaagaag gtaaccacac cacggcatac cgtgatggtg cgggtatctg
      181 gaccatttgt cgtggtgcca ccagggtgga tggcaaacct gtcgtcccgg gcatgaagtt
      241 gtcgaagaaa aaatgcgacc aggttaacgc cattgagcgt gataaggcgc tggcatgggt
      301 ggagaaaaac atccgggtgc cactgaccga accccagaaa gcggggatcg cgtcattctg
      361 cccgtataac attggtcccg gtaagtgttt cccgtcgacg ttttataaac gaattaatgc
      421 aggcgatcgc agaggagcgt gtgaagcgat tcgctggtgg attaaggacg gtggcagaga
      481 ctgccgtatc cgctcaaata attgctacgg tcaggtatct cgtcgtgacc aggagagtgc
      541 gctggcgtgc tggggtatcg acagataagc agaatatttt gctgaaaaat gacgttggcc
      601 aacgcggacg gataacacga aatcctgcga actggcaaaa tgtaagtgaa taaagtcagg
      661 atcattattt cacgcagagg caccgtaatg gtgcctttgt catttctgcg cttcgcacaa
      721 gcgtaaataa accaaagaac ctttcaggat gagccctggt ggataaccgg cagtggtctg
      781 gttaaccctc tttgggctgg ttattcctgt gcgcagggtt catcactaaa aggaatcaac
      841 catgaaagag atgatttctg tcgatcatga aatatccatg agtagtctgg attttctgaa
      901 taacattatt aaccccatcc gggttgaggc agggaaacca cctcatgaac cgcgtaagtt
      961 ccttgcaaaa attgaggacg aacttgagct tgaggtaacc tggaaaaaat tccggttaaa
     1021 caataaccag acaagaacgg catactatga tctggatttt gaccagatga tgctcgttgg
     1081 catgagggag tcaaaggccg ttcgtcgttc tgtgctggca aggctcaaag cgatgcatgg
     1141 tattcagatc ccacggactt tacctgaggc gttgcgattt gcggcaaaac tggctgaaca
     1201 gaaagcagtg ctggaaaatc aactggcaat agcagcgccg aaggctgaat ttgttgataa
     1261 ctatgttgaa gcatctggtc tgatgggatt ccgggaagtt gctaagttac tcggtatcaa
     1321 agaaaccgat ttccggctgt ttttgttgga gaacggaata atgtatcgcc tcgctggaaa
     1381 aatgacgcct tactcgcatc acctggatgc ggggcggttt agcgtgaaaa cgggcgaggc
     1441 gggcaatggt catgctttta cgcaggttaa attcacccca aaaggtgttc agtggattgc
     1501 tggtctgctt gctgcatgga gagctaccgc agcatgaaga tgataaaaat ggactggaaa
     1561 tttttgctgg tctggctgat tccgttttta tgggtggttg cccggttaat tactgctatt
     1621 aaggggtaaa gatgtcagac aaactcataa tgctggcgaa gggcctttgt gtaatcgtcg
     1681 gtatttcatt ttcactaatg ctggttgttc tttttctttc cataacctgg atggcgttga
     1741 cttcggcagg gctggtgggg tgagcataaa ccgaatgctt tccgcgttta ccgttattct
     1801 gcttgtggtc tgtggtgcgc ttagtctggg gctgaatcat taccgcgata atgccatcac
     1861 ctacaaagcg cagcgcgata aaaaagccag agagctggag caggcaaacg caaccattac
     1921 tgacatgcag cagcgccagc gcgatgttgc tgcgctcgat gcaaaatact cgagggaatt
     1981 agccgatgcg agagctgaaa atgaaactct gcgtgctgat gttgccgctg gtcgtaagcg
     2041 cctgcggatc aacgccacct gccccggtac cgtgcgtgaa gccaccggca cctccggcgt
     2101 gggcaatgat gccgccgtcg aactctctcc ggttgctgga cgaaacgttc tcggtatcag
     2161 agacggaatc atcagcgacc aggcagcatt gagaatgctt caggaatata tccgcactca
     2221 gtgtattaac tagtattttt gttattcgga gaatgcatga agaaattact ggtaaccgta
     2281 aagccttttc agggaacaat tccgttccgt attttgcagc gtggtcgtgt tcttgttgaa
     2341 ggttcgttca gtggtaaatg tacgcaatta cactcccgga cctttcaggt gaatgccacg
     2401 aatgaagagc taaccgttga gtgtacgatg aatgccgcta aatgccgtat ggtatcggct
     2461 gcattacagc cagtgtgttg agcgacctta ttatccatgc gcggtattgt cgccgtattc
     2521 ccgtattaac agagaccgca gcccgacagg gagactcctc tgcgcgagtg tgcggggata
     2581 atcaaaaacg atacacaccg gggtttaccg cgttaacgga gcgcggcgtt gtcctctcat
     2641 ggtcgctggt ccggtgcgat ggtggaagaa accggacgat gtgttacctc acaagacctg
     2701 ttatgtcatg tgtctgattt gtgatttaag tcggataatt gtcgttgcca ttaagcagag
     2761 gattgatggc cgacagggtg gcattgttag aataagactt attcttatct gtgccgggaa
     2821 tgaaaatgaa aagaaatctt ccgttaatta ttttgttgtc ttctctggtt atgggctgta
     2881 cgcaacataa aacagatatg ccccgacagt tggttaaggc attaccacaa tatccggcct
     2941 atgcagcggc aaattatata aagggacggg ttgatgtgaa gtttgatatt ggtgctgatg
     3001 gtactgtcac ccgaattgag tttatccgtt cagagccgca ccatctgttt gatgagcagg
     3061 ttgtaaaggc gatggcaaaa tggcgatttg agaaggacag gccgcgtaaa ggcgtgaaga
     3121 aaacgtttat ctttagtcct tctgcaccct gattatttca tcagaaatta attatcactc
     3181 tgttgttatt ctgtacatcc cggctgggta agtcttgttc cgccgggtat gaagatgaaa
     3241 tattgttgga ggacagtggg tacctgctcc tgtaaccgaa cgttcatttc tcgttatttg
     3301 tcatgctggc cggacgcaga tgcgttgcat ctgttgccag ccttctcctg caggcttcaa
     3361 taacccacgc tgaaaagtta ccggaccctt tatgctcaag ggcgatgttg atctgttcaa
     3421 tcatgtgatt ggggaaacgg atattgcggg ttgtggttct gcgggtccgg tttttcgatg
     3481 acatatttat ttcctttact gattgccata tgacggggat tttacatggc tgagcttcgt
     3541 acactccaga gcagaatcaa aacactgaat acccgacggg tgaatattct gaagggtgaa
     3601 cagcgtcgtg tcagtggcag tgcacgtgtt tccctcaagc gtcatatctg gctcagggat
     3661 gccgggcagt gctgtctctg tggtcgtgtg gttgacctct gtgacagtga actcgatcac
     3721 cgaattgcac ttcagttcgg tggtggtaat gaggagacga atctctggac gctctgtacc
     3781 gaatgccatc gacaaaagtc tgctcgtgaa gcggcgggtg gtatgccgga cccgacgctg
     3841 ccggaggtgt ccggaggtag tggcagagcg gacgacatca tcggactgta acccgaccgg
     3901 gggggggtat cattcggcgt aaaaaacgat cgctccggac accgcgcccc ctctcacgca
     3961 gagaaaaaat tccccttcag aagttataaa catgtttata acctactgat ggatttttac
     4021 cgtttttttc tttttattca taacgttgtg ggaggtaaat gtttatactt tttttcgcaa
     4081 aatgtttaca gggcagcggt aaacattttc cctgccgcct ccattcttac cgggaggatt
     4141 gctgacgttt ttctgttttt ccccggagga aagaatgctg acgacgcaaa aaagaaaatt
     4201 tgcgctggcg ctcatgtcgg gcaaaaacag aacggctgct gctattgagg ctggttactc
     4261 ggaaaagtcc gccagatgta aaggctcaca actggcaaaa gatccggaag tgctcgcgtt
     4321 tatagcccgt aagcagggcg agacggtgga ggtggatgag gttcctgttt accggcagaa
     4381 aaagacgcag gcgtgccacc gggaagttcc tccggtggaa aagaaaaacc aggtttcagt
     4441 gccaccggat gacgggcaaa gtttctctcc actatccggt attgattaca tggaggacgg
     4501 ccttcctgat cctgtgaaag ccatgggtcg gatcctggtg gaaaacatca gcattgatcc
     4561 gaaactggct ctggatgcag cctggcgact ggcgcagttc acgcaccaca aaaaagggga
     4621 tgccggtaaa aaatcggcaa aaggtgacgc ggcgaaaaaa gcggctaacc gttttgcggt
     4681 gccaccacca ccccgcctgg tggtgaataa tgataatgag ggcaacggat gatacctgtg
     4741 tggagcacgg cctgcccgga ctgggcagag cgtctgaaaa aggggctgtc gattattccg
     4801 gctccgattt acccggacca ggccgcacat gccctggcga tttttaaaca actgcggatt
     4861 gtggatgcac cgggcagccc gacgttcggg gagtcctgtg caccgtgggt gtttgatctg
     4921 gtggctgccc tgtttggctc ctacgatgcg cagaccggtg tacgccatat aaaggaagtg
     4981 ttcattctga tacccaaaaa aaacagcaag tccacgctgg ctgccgggat catgatgacg
     5041 gcgctgttac tgaactggcg gcaggcggcg ggctacacca ttctggcccc gaccgtggag
     5101 gtggcggcta acgccttcaa ccctgccagg gatatggtac gacgggacga tgatctggat
     5161 gacctctgtc aggtgcagac acatatccgg accatcaccc atcgggtgac ggacaccacc
     5221 cttaaggtgg tggctgccga tccgaatacg gtgtccggta tcaagtccgt ggggacgctg
     5281 attgatgaac tgtggttatt tggcaagcag tacaaagcgg aggacatgtt acgtgaagcc
     5341 atcggcggcc ttgcctcccg cccggaaggg tttgtggtgt acacaaccac ccagtcgaat
     5401 gagccgcccg ccggggtgtt cagacagaaa ctgcagtacg cccgggatgt gcgcgacggc
     5461 aaaattaatg atccgcactt tctgccggtg atatttgagc atccgccgga gatggtggag
     5521 cgcggtgagc acctgctgat ggaaaacctc gccatggtta acccgaatct cggttattcg
     5581 gtggatgagg cttttctgta ccgggagtac cgtaaagccc gggaggctgg tgaggaggca
     5641 tttcgtggct tcatgtcaaa acacgccaat gtggaaattg gtcttgccct gcgttctgac
     5701 cgctgggcgg gtgcggattt ctgggagcag cagggcaggc gcgtcagcct ggacgatatc
     5761 ctgcagcgcg ctgatgtggt gacggtgggg attgacggcg ggggcctgga tgatctgctg
     5821 ggaatgtacg tgattggccg tgacagggaa acccgcgaat ggctgggctg gggccatgcc
     5881 tgggcgcatg aaaccgcggt ggtcagacgg aagagtgagg catcccggtt tcaggatttt
     5941 gtggccagtg gagacatgac gattgtccgt cgggtcgggg atgacacggc ggaagtggcg
     6001 gagtatgtgc gtcgtattca tgaggctgag ttactggacc atatcggtat tgacccgtca
     6061 ggtgtggggc agattctgga ttcactggca gaagccggga tccccgacgg aattgttgtg
     6121 gggataagcc agggctggaa gctgggcggg gccatcaaaa ccaccgagcg caaactggct
     6181 gagggagtgc tggtgcatgg tggtcagcca ctgatggcct ggtgcgttgg caatgcccgg
     6241 gtggagccta aaggtaacgc catccttatt accaaacagg ccagcggacg ggggaaaatt
     6301 gacccgctga tggcgctgtt caatgcggta tccctgatgt ccctgaatcc ggagcccaaa
     6361 aagaaagaat atgcggtttt tttcatataa ccctgttcac cctgtaacca tcatggaccg
     6421 ctgcggcggt ttttttattt tcaggaggct gatgtgactc ttaaacgggc ctgctccctg
     6481 ctgacggtga aatccttcag tgaggatgag cgggtgatca ccgggattgc gtcaacgcct
     6541 tctccggatc gggatggtga catcctggag ccggagggcg cggagtttgg cagtgcgatc
     6601 ccgtttctct ggcagcatga ccattcccgc ccggtggggc agtgtacggt gcgccgggtc
     6661 agcgaagggc tggaaatcac ggcaacactg gtgaaacccg taccggatat gccgtcgcaa
     6721 ctggctgccc ggctggatga ggtctgggcg gccattaaga ccgggctggt cagggggctg
     6781 tccgtgggct tccgtcctca tgaatacacc tttctggacg gaggcggagt gcattttctg
     6841 cgctgggagc tgatggaggt gtctgccgtc accgtgcccg cgaatgcgga atgcactatc
     6901 cggaccatta aatcttacga ccgcccgttt tctgccgcgt ccggcaaccg gaaaccggtg
     6961 gtgaaaatcg catcttctgc cggcgctgcg gcacagtcaa caaccgtttt tcataaggaa
     7021 aagaccataa tgaatattgg cgaacagatt aaaagttttg aaaacaagcg tgcagcgctg
     7081 gcagcctccc ttgaggaggt catgaccaaa gccgcagagg aagggcgcac gctggatgtg
     7141 gaggaggaag agcattacga caacaccgca gcggaaatcc gtcaggtgga tgcgcacctg
     7201 aagcgcctgc gtgaactgga agccggtaag gccgccacgg cgcagccggt gaaacaggcc
     7261 ggtaacggga atgtggccgc ggtggcttct gcgccggtga tccgtgtgga gcagaaactg
     7321 gataagggga ttggtttcgc acgttttgcc aaatcgctgg ctgcggctaa aggtgtccgc
     7381 tctgaagccc tggaagtggc ccgtcgtcag tatccggatg acagtcgtct gcatcatgtc
     7441 ctgaaatcgg cagtgggcgc ggggaccacc acggatccgc agtgggcagg cagcctgtct
     7501 gaatatcagg aatacgcaca ggactttatt gattacctgc gtcctcagac cattatcggg
     7561 cgatttggtc agggcgggat ccctgcactt cgtcaggtgc cgttcaatat ccgtgtgcac
     7621 gcccaggtgt ccggcggtgc tgccggctgg gtgggtgagg gtaaggcaaa acccctgacg
     7681 aagtttgatt ttgaatccat caccttcagt catgcgaagg tgtcggccat tgcggtactg
     7741 acggaagaat tgatccgttt ttccagtccg gctgctgatg cactggtccg taatgcgctg
     7801 gcggaagcgg tggtggcgcg tctggataca gactttgtgg acccgaaaaa agcggcggtg
     7861 gcagatgtct ccccggcgtc catcacccat gatgtgaagg gcacggcatc aagcggtaac
     7921 ccggatgcgg atgcagaggc tgcgtttgga cagtttgtgg cagcaaacct gcaacccacc
     7981 ggtgcggtct ggctgatgtc cagcaccaat gccctggcac tgtccatgcg taaaaatgcg
     8041 ctgggtcaga aagaataccc ggacatgacc ctgctgggtg gctccttcca ggggctgccg
     8101 gtgattgtct cccagtacgt gggtgaccag ctggtgctgg tgaatgcccc ggatatttat
     8161 ctggcggatg acggcggcgt ggcagtggat atgtcccgcg aggcatcact ggaaatgcag
     8221 tctgagccga ccggcgacag taccacgccg tcgccggtgg agctggtttc catgttccag
     8281 acaggcagtg tggccatccg tgcggagcgc tggatcaact ggcgtcgtcg ccgtaccgcg
     8341 gcggtggcgg tgatcaccgg tgtgaactac ggcagtgcgt ccggcggctg agtctgataa
     8401 ggaggacggg aggcgtgcgc ctcccgtaac aggttatggc aaagatccga tacctgcagg
     8461 gcacgcatga tgcccgggcc ggggatatcc gggatgtggc acagccgtgt gcggaggtgc
     8521 tggttcgcct gggaaaggca gagtacatca cggtgcgacg tccggcaggt cagaaaaaga
     8581 aacgtgatgc ggagcatggc gaatgtggaa ccttttacgg cgaacccgaa aaaaccagaa
     8641 atcaggacgt gacgtaagag aggcgggctg gaccagcctg tttcaggcgg tggctgagcc
     8701 cttttccggc gcctggcagc agggcgtgaa agccgatcct gaagccgtcc tctcctttca
     8761 tgcggtgttt gcatgtattt cgctgatatc ccaggatatc gccaaaatgc ggctgcgtct
     8821 tatgcagacg gatgcgcagg ggatacgcag ggaaacgcgc cggggagata ttgcccgcct
     8881 ctgtcgtcgt cccaacgccc agcagaaccg catccagttt tttgaactgt ggctgaacgc
     8941 caaactgcgt cacggcaata cggtggtgct gaaaatccgt aatgcccggg ggcagatcaa
     9001 agaactgcgt attctggact ggaaccgggt tgaacctctg gtggcggatg acggcgaggt
     9061 gttctaccgc atcacgccgg accggaactg tgggatcact gaggcggtga cggtgcctgc
     9121 ccgggaagtg atccacgatc ggtttaactg tttttttcat ccgcttatag ggttgccgcc
     9181 ggtgtatgcc gccgggctgg cggccacgca ggggcatcat attcaggaaa attcgacgtc
     9241 ttttttcaga aatggcggca ggccgtccgg ggtgattgag atccccggca gtattacgga
     9301 agaaaatgcg aaaaaactga agagcaactg ggacagcggg tatacaggcg aaaatgcggg
     9361 gaaaacggcc attctgagta acggggcaaa atacaacccc acgacgtttt caccggtgga
     9421 tgcgcagacg gtggaacaac tgaagatgac cgctgaaatt gtctgttcgg tgttccgtgt
     9481 cccggcctac aagattggcg tgggacaacc gccttccagt gacaacgtgg aggcgctgga
     9541 gcagcagtat tattcccagt gcctgcagac actgattgag tccattgagc tgttactgga
     9601 tgaggcgctg gaaacggggg aaaacgagag tacggaattt gatgtcacca cgctgctgag
     9661 aatggacagt gagcggcgca tgaaaacgct gggggatgcg gtgaaaaata cgcttctcac
     9721 gcccaatgaa gcccgtaaac gggagaacct gccgcccctg gcaggaggcg atgcactgta
     9781 tcttcagcag cagaactaca gtctggaagc gttgtcccgc cgtgatgccc gtgaagatcc
     9841 gttcgcgtcg tccggtaaaa cagcttccgt gcctcaggcg gtcgctgcat ctgacggtaa
     9901 taaggcaatc actgaaacag agcatgacgc ggtgaaggcg atgttcaggg ggattctgaa
     9961 aaaatgaatg aacgtgaact gtccattatc cgtgcgctgg gtgaagaatt ttccgcggtg
    10021 ctggcggatt tacagcgcac atttgaggag aaaatagccg cgcaggcaca aacgtttgaa
    10081 gaaaaactgg cttccctgtc tgtggtatta cagaagtgcg tgacgggcga tgatgtgcgt
    10141 ccgatgcttg agcagatggt gaaggaggcg gtaagccata tccctgttcc gcgagatggt
    10201 cgtgactacg atccggaagt actgcagaag gcggtgaatg atgcggtcgc aaatattccg
    10261 cagccggcgg acggtaaaag tatcaccccg gatgatgtgc gtccgatgct tgaacagatg
    10321 gtgaaggagg ctgtaagcca tattcctgtt ccgcgtgatg gtcgtgacta cgatccggaa
    10381 gtactgcaga aggcggtgaa tgatgcggtc gcaaatattc cgcagctggc agacggtaaa
    10441 agcctcaccc cggatgatgt gcgtccgatg cttgagcaga tggtgaagga ggcggtaagc
    10501 catattcctg ttccgcgtga tggtcgtgac tatgatccgg atgttctgca gaaggcggtg
    10561 aatgatgcgg tgagtgccct gccggctccg caggacgggc gtgatgccac ggcactggaa
    10621 atactccctg ccattgacga tcaaaaatcc tttccccggg gcacgtatgc cacacaccag
    10681 ggcggactct ggcgggcgta tgaaaaaacg cacgggatgc ggggatggga atgcctggtt
    10741 gacggggtgg cggatattga cgtcagcatg acgggtgaac ggttgttctc tgtggtggtc
    10801 cggcagagca gtggccagcg tacggaaaaa acattttccc tgccggtgat gctctaccgc
    10861 ggtgtgttca gagccggtga aacctaccac cccggcgata cggtgacgtg ggggggctcg
    10921 ctgtggcact gcaacagtat gaccggtgat aaacccggag aagctcattc atcaggctgg
    10981 accctggctg caaaacgtgg gcgggatgct ggaggcggaa aatgacggca ttactgacac
    11041 tggaagagat caaggcacat ctgcgtgtcg accatgacgc ggatgatgac atgctgatgg
    11101 acaaggttcg tcaggctacc gccgtgctgc tggcctacat tcagggcagc cgggataagg
    11161 tgattcgtga ggacggtgaa ctgatcccgg gcgaggcatt aacccggatg aagggggctg
    11221 ccatgcgact gaccgggatg ctgtaccgga atccggatct tgcggagcgg gaagaactgc
    11281 ttcaggggga gctgccgttt tctgtttccg tgctgattta cgatttgcgt tgtccgacgg
    11341 tgttatgagg agggggaatg gcaatatctg caggtcgtct gacacagatg ataagtgttc
    11401 tgaacccggt gttaacccgt aatgctgccg gagaaatgac ggaagaatgg gtgtcatgcg
    11461 ggaaaattca tgcggatatc cgtggcagga gcagccggga gcggatgcag tccggtgcgg
    11521 aaatggcgca ggcggaaatc cgcatctggg tgcgcggtca gtccggtcgg gaaatcacgg
    11581 cagcgtcacg acttcatgtg ctgagtggtc catggcgtga ccggatcctg aacgttgtcg
    11641 ggctgcccgt gccggatgcg accggcgggc gtctggaaat tctctgtcgg ctgggagggg
    11701 aaaaatgatc gaaaccctgc tggatttttc ggggctggag gagatcagcc gcgatttgca
    11761 gcttctgagt ggtgcggaaa ataaccgggt gctgcgtgag gcaacccgtg cgggtgcgaa
    11821 tgtgctgaaa gaagaagtgg cgtcacgggc accggtacgc aggggaaaac tgcgccgcaa
    11881 tgtggtggtc ctttcccggc gctcccgcga tggcgggatg gaatccggtg tccatatccg
    11941 tggtgttaat ccgga
//
LOCUS       DAZGHF010000139        11854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGHF010000139 DAZGHF010000000
VERSION     DAZGHF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.140"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            47..916
                     /locus_tag="ACHK3A_004074"
     CDS             47..916
                     /locus_tag="ACHK3A_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO8742090.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMVENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(966..1319)
                     /locus_tag="ACHK3A_004075"
     CDS             complement(966..1319)
                     /locus_tag="ACHK3A_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO8742091.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1457..3103)
                     /gene="groL"
                     /locus_tag="ACHK3A_004076"
     CDS             complement(1457..3103)
                     /gene="groL"
                     /locus_tag="ACHK3A_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO8742092.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3147..3440)
                     /locus_tag="ACHK3A_004077"
     CDS             complement(3147..3440)
                     /locus_tag="ACHK3A_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO8742093.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3716..4972
                     /gene="yjeH"
                     /locus_tag="ACHK3A_004078"
     CDS             3716..4972
                     /gene="yjeH"
                     /locus_tag="ACHK3A_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO8742094.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLSRLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(4988..5464)
                     /locus_tag="ACHK3A_004079"
     CDS             complement(4988..5464)
                     /locus_tag="ACHK3A_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO8742095.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5801..7237
                     /gene="aspA"
                     /locus_tag="ACHK3A_004080"
     CDS             5801..7237
                     /gene="aspA"
                     /locus_tag="ACHK3A_004080"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO8742096.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7356..8657
                     /gene="dcuA"
                     /locus_tag="ACHK3A_004081"
     CDS             7356..8657
                     /gene="dcuA"
                     /locus_tag="ACHK3A_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO8742097.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8773..9111
                     /gene="cutA"
                     /locus_tag="ACHK3A_004082"
     CDS             8773..9111
                     /gene="cutA"
                     /locus_tag="ACHK3A_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO8742098.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9087..10784
                     /gene="dsbD"
                     /locus_tag="ACHK3A_004083"
     CDS             9087..10784
                     /gene="dsbD"
                     /locus_tag="ACHK3A_004083"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO8742099.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIANVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10821..11396
                     /locus_tag="ACHK3A_004084"
     CDS             10821..11396
                     /locus_tag="ACHK3A_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8742100.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDAWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11503..11578
                     /locus_tag="ACHK3A_004085"
     tRNA            11503..11578
                     /locus_tag="ACHK3A_004085"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11536..11538,aa:Phe,seq:gaa)
BASE COUNT         2827 a         3072 c         2927 g         3028 t
ORIGIN      
        1 aattaaaatt aattatttaa ttgagaaatg gttagggaga acctacatgg ccataagcat
       61 caagggtgtt aataccggtg ttattcgcaa gagcaataat ttcattgcac tggcgctgaa
      121 aattaaagag cctcgcaata aagaatcgtt gttctttctg tctgtcatgg aactgcgcga
      181 ccttctgatt gcgctggaaa gccgtctgca tcaaaagcat aaactggatg ctgctgcacg
      241 ccttcagtat gagcaagctc gcgataaagt cattaagaaa atggtagaaa atatcccaga
      301 aattctggtt gatgagctta aaaatgccga tattaatcgt cgggttaaca cgctggaact
      361 taccgataat cagggcgaaa atcttacctt cgtattaaca ctacatgacg gtagcaaatg
      421 tgagttagtc atcaatgaat tacagattga aatgctggcg cgagcaatca ttcatgcaat
      481 taataatgcg gaaatgcgtg aactggcatt acgtattact tcactgttag atttcctgcc
      541 gttatacgac gttgactgcc aggacaatgg caatctggaa tatgacacct attcccaacc
      601 ggaatggaaa cataacttgt tcaaccatta tctggcggtg ctttaccgct ttaaagatga
      661 aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg cgtgaagcaa cgccgggtaa
      721 agaagtggag gcgatcactc gccgaatgct tgatttcagc ccccgactga aaaaactcgc
      781 gggtgtgcca tgtcaggtct atgttcgcac cgtagcggca aataacgccc aaccgctaac
      841 ccaggatcaa tgcttacgcg cgctgcatca cttacgtgtt caatccacca gcaaaactgc
      901 accacaggcg aaataatctc atctgtttta ctacgaggct ggaagtatcc agcctctgga
      961 tcacatcagt tcgggcactt ataaacctga ccgataatct ggctatccgt cgggacaaaa
     1021 ctggacaaca ttccctgcga cgggctgctg atgccataaa tcacgttacc gcccattgca
     1081 gccgcctggt tgcgcagatc gtttgctgcg ccgcgcatag aaccgccctc ttctccgtgt
     1141 tgcccggaaa gccagttgct ttgcttacct gtcgcagtac caatcagctg gcactctgcg
     1201 cctggctgct cgtccacaat gcgtacactc tgaccggcag cactcaattc gttgctggag
     1261 ctacaacccg ccatcagtag cgcggcaccg acaatccctg ctaagtattt tacgtgcatg
     1321 ttattcccca taatcaatga gctggacgcc actcgcgtcg tccgtgtctg aatcttatac
     1381 tagaaagatg accaaaagaa aaacccccag acatttctgc ccgggggttt gtttatttct
     1441 gcgaggtgca gggcaattac atcatgccgc ccatgccacc catgccgccc ataccgccag
     1501 cagcgcctaa gtcagctgca tcgtttttcg gcaggtcggt aaccatgcat tcggtggtga
     1561 tcatcaggcc agccacagaa gctgcgtact gcagagcaga acgagttact ttggttggat
     1621 ccaggatacc catgtcgatc atgttgccgt attcttcggt tgctgcgttg taaccgtagt
     1681 tgccgtcgcc gcctttaacg gtgttagcaa caacagacgg ttcttcgccg cagttcaata
     1741 cgatctgacg cagcggagct tccattgcac gcagtgcaac tttgataccc acgttctggt
     1801 cttcgttctg accacgcagg tcagccagtt tagacgctac gcggatcagc gcaacaccac
     1861 caccagcaac cacgccttct tctaccgcag cacgggtcgc gtgcagggca tcttcaacgc
     1921 gtgctttttt ctctttcatt tcaacttcgg tagcagcacc cactttgata actgcaacgc
     1981 cgcctgccag tttcgctacg cgctcctgca gtttttcacg gtcgtagtca gaagttgctt
     2041 cttcaatctg ctgacggatc tgagcaacac ggccctggat tgcagcttct tcacccacgc
     2101 catcaatgat ggtggtggtg tctttgttga tcacaacacg tttagcctga cccaggtctt
     2161 ccagggttgc tttttccagc tccataccga tctcttcaga gattacggta ccgccagtca
     2221 gggttgcgat atcctgcagc atagctttac gacgatcgcc gaagcccgga gctttaaccg
     2281 cagcaacttt cacgatgcca cgcatggtgt taacaaccag agttgccagc gcttcgcctt
     2341 ctacatcttc agcgatgatc agcagcggtt tgcctgcttt ggcaacagct tccagaaccg
     2401 gcagcatttc gcggatgttg gagattttct tgtcagccag caggatgaac gggctttcca
     2461 gttctactgc gccagtttcc ggcttgttga taaagtaagg agacaggtag ccacggtcga
     2521 actgcatacc ttcaaccacg tccagttcgt cctgcagacc ggtaccgtct tcaacggtga
     2581 taacgccttc tttaccgact ttgtccatcg cttcagcgat cagtttacct acggtttcgt
     2641 cggagttagc ggagatggta ccaacctgag caatcgcttt agagtcagag catggtacgg
     2701 acagcgcttt cagttcttca actgcagcgg taaccgcttt gtcgatacca cgtttcaggt
     2761 ccatcgggtt catgcccgca gcaacagctt tcagaccttc agtgatgata gcctgagcca
     2821 gtacggttgc agtggtggta ccgtcgcctg cagcgtcgtt cgctttggag gcaacttctt
     2881 tcaccatctg cgcacccatg ttttcgaact tgtcttccag ttcgatttca cgagcaacgg
     2941 aaacaccatc tttggtgatg gtcggtgcac cgaaagattt atccagaact acgttacggc
     3001 ctttcggacc gagggtaact ttcactgcat ctgccagtac gtttacgccg cgcagcattt
     3061 tcacacgagc gtcgttaccg aattttacgt ctttagctgc cattatcttt attccttaaa
     3121 ttcgtatgtt cagtgtcgtg cgcggattac gcttcaacaa ttgccagaat gtcgctttcg
     3181 gacatgatca acacttcttc attgtcgatc ttctcggatt tcacaccgta gccatcgttg
     3241 aaaataacga tgtcgccaac tttcacgtcc agcggcttca cttcgccatt ttcaaggata
     3301 cggccattgc cgacagccag cacttcgccg cgggtggatt tagccgctgc agagccggtc
     3361 agaacgatgc cgccagcaga tttagtttca acttctttac gcttgacgat cacgcgatca
     3421 tgcaatggac gaatattcat tgataactct cctttgagaa agtccgtatc tgttatgggt
     3481 gacgccggag cttacgtggt ttcccggctg gtgaccagag aaatggggat gaggcttcgc
     3541 cccttcaagg gggaaaaaga aaaaaaattc tgatcagcac aaaatcgggt gaaaaccctg
     3601 attcacctca catttcatcg caatttcttc atcgccgtca taagcgaatc tgattgtgct
     3661 accatcgaaa atctacgcag ttgcccaaaa tttgggcgca atcggacatc aacccatgag
     3721 tggactcaaa caagaactgg ggctggccca gggcattggc ctgctatcga cgtcattatt
     3781 aggcactggc gtgtttgccg ttcctgcgtt agctgcactg gtagcgggca ataacagcct
     3841 gtgggcgtgg cccgttttga ttatcttagt gttcccgatt gcgattgtgt ttgcgattct
     3901 gggtcgccac tatcccagcg caggcggcgt cgcgcacttc gtcggtatgg cgtttggttc
     3961 gcggcttgag cgagtcaccg gctggctgtt tttatcggtc attcccgtgg gtttgcctgc
     4021 cgcactacaa attgccgccg ggttcggcca ggcaatgttt ggctggcata gctggcaact
     4081 gttgttggca gaactcggta cgctggcgtt ggtgtggtat atcggtactc gcggtgccag
     4141 ttccagtgca aatctacaaa ccgttattgc cggacttatc gtcgcgctga ttgtcgctat
     4201 ctggtgggcg ggcgatatca aacctgcgaa tatccccttc ccggcacctg gtaatatcga
     4261 acttaccggg ttatttgctg cgttatcagt gatgttctgg tgttttgtcg gtctggaggc
     4321 atttgcccat ctcgcctcgg aatttaaaaa tccagagcgt gattttcctc gtgctttgat
     4381 gattggtctg ctgctggcag gattagtcta ctggggatgt acggtagtcg tcttacactt
     4441 cgacgcctat ggtgaaaaaa tggcggcggc agcatcgctt ccaaaaattg tagtgcagtt
     4501 gttcggtgta ggagcgttat ggattgcctg cgtgattggc tatctggcct gctttgccag
     4561 tctcaacatt tatatacaga gcttcgcccg cctggtctgg tcgcaggcgc aacataatcc
     4621 tgaccactac ctgtcacgcc tctcttctcg ccatatcccg aataatgccc tcaatgcggt
     4681 gctcggctgc tgcgtggtga gcactttggt gattcatgct ttagagatca atctggacgc
     4741 tcttattatt tatgccaatg gcatctttat tatgatttat ctgttatgca tgctggcagg
     4801 ctgtaaatta ttgcaaggac gttatcgact actggcggtg gttggcgggc tgttatgcgt
     4861 tctgttactg acaatggtcg gctggaaaag tctctatgcg ctgatcatgc tggcggggtt
     4921 atggctgttt ctgccaaaac gaaaaacgcc ggaaaatggc ataaccacat aatccggcgt
     4981 ttcgacatta atcctggcga tcgtctttat gatcaaggcg gtcgcggtca tcatcctttc
     5041 gctggtactc accatcaaaa gtattaccgc caccggtccc ggcgctaaaa ccgccgcctg
     5101 gcatgcgaga aaagcgcaaa tgcggcatca acttcactgt cagatgcttt tgcaccggcg
     5161 gcaacaaaag cagaagaccg aggaagtcgg tgaaaaagcc cggtaataaa agcagcaaac
     5221 cggcaatgat cagcgaaaca cttttaatca tctccgccgc tgggttttca cccgccgcca
     5281 ttttttgctg catcagcacg aaattcttaa agccctggtt acgtaccagt gacataccga
     5341 taactgacgt gaatataacc agcacgagag tcagcaatac ccccaataca tgggcaacct
     5401 gaataaagat tgaaatctca atatagacat aaaggaaaat ggcaataaaa ggtaaccagc
     5461 gcaaaggttt ctcctgtaat agcagccggt taaacccggc tacctgaatg ggttgcgaat
     5521 cgcgttcagc ttatattgtg gtcattagca aaatttcaag atgtttgcgc aactattttt
     5581 ggtagtaatc ccaaagcggt gatctatttc acaaattaat aattaagggg taaaaaccga
     5641 cacttaaagt gatccagatt acggtagaaa tcctcaagca gcatatgatc tcgggtattc
     5701 ggtcgatgca ggggataatc gtcggtcgaa aaacattcga aaccacatat attctgtgtg
     5761 tttaaagcaa atcattggca gcttgaaaaa gaaggttcac atgtcaaaca acattcgtat
     5821 cgaagaagat ctgttgggta ccagggaagt tccagctgat gcctactatg gtgttcacac
     5881 tctgagagcg attgaaaact tctatatcag caacaacaaa atcagtgata ttcctgaatt
     5941 tgttcgcggt atggtaatgg ttaaaaaagc cgcagctatg gcaaacaaag agctgcaaac
     6001 cattcctaaa agtgtagcga atgccatcat tgccgcatgt gatgaagtcc tgaacaacgg
     6061 aaaatgcatg gatcagttcc cggtagacgt ctaccagggc ggcgcaggta cttccgtaaa
     6121 catgaacacc aacgaagtgc tggccaatat cggtctggaa ctgatgggtc accagaaagg
     6181 tgaatatcag tacctgaacc cgaacgacca tgttaacaaa tgtcagtcca ctaacgacgc
     6241 ctacccgacc ggtttccgta tcgcagttta ctcttccctg attaagctgg tagatgcgat
     6301 taaccaactg cgtgaaggct ttgaacgtaa agctgtcgaa ttccaggaca tcctgaaaat
     6361 gggtcgtacc cagctgcagg acgcagtacc gatgaccctc ggtcaggaat tccgcgcttt
     6421 cagcatcctg ctgaaagaag aagtgaaaaa catccaacgt accgctgaac tgctgctgga
     6481 agttaacctt ggcgcaacag caatcggtac tggtctgaac acgccgaaag agtactctcc
     6541 gctggcagtg aaaaaactgg ctgaagtcac tggcttccca tgcgtaccgg ctgaagacct
     6601 gatcgaagcg acctctgact gcggcgctta tgttatggtt cacggcgcgc tgaaacgcct
     6661 ggctgtgaag atgtccaaaa tctgtaacga cctgcgcttg ctctcttcag gcccacgtgc
     6721 cggcctgaac gagatcaacc tgccggaact gcaggcgggc tcttccatca tgccagctaa
     6781 agtaaacccg gttgttccgg aagtggttaa ccaggtatgc ttcaaagtca tcggtaacga
     6841 caccactgtt accatggcag cagaagcagg tcagctgcag ttgaacgtta tggagccggt
     6901 cattggccag gccatgttcg aatccgttca cattctgacc aacgcttgct acaacctgct
     6961 ggaaaaatgc attaacggca tcactgctaa caaagaagtg tgcgaaggtt acgtttacaa
     7021 ctctatcggt atcgttactt acctgaaccc gttcatcggt caccacaacg gtgacatcgt
     7081 gggtaaaatc tgtgccgaaa ccggtaagag tgtacgtgaa gtcgttctgg aacgcggtct
     7141 gttgactgaa gcggaacttg acgatatttt ctccgtacag aatctgatgc acccggctta
     7201 caaagcaaaa cgctatactg atgaaagcga acagtaatcg tacagggtag tacaaataaa
     7261 aaaggcacgt cagatgacgt gccttttttc ttgtgagcag taacttaaaa ataacaatct
     7321 aatatcaact tgttaaaaaa acaaggaagg ctaatatgct agttgtagaa ctcatcatag
     7381 ttttgctggc gatcttcttg ggcgccagat tggggggaat aggtattggt tttgcaggcg
     7441 gattgggggt gctggttctt gccgctattg gcgttaaacc cggtaacatc ccgttcgatg
     7501 tcatttccat tatcatggcg gttatcgccg ctatttctgc catgcaggtt gctggcggtc
     7561 tggactatct ggttcatcag acagaaaagc tgctgcgccg taacccgaaa tacatcacga
     7621 tcctcgcacc gatcgtgacc tatttcctga caatctttgc tggtactggc aacatctctc
     7681 tggcgacact gccagttatc gctgaagttg cgaaggaaca aggcgttaaa ccttgccgtc
     7741 cgctgtctac tgcagtggta tccgcgcaga ttgcgatcac cgcatcgcca atctcagcgg
     7801 cagtggttta catgtcttcc gtaatggaag gtcatggcat cagctacctc catctgctct
     7861 ccgtggtcat cccgtccacc ctgctggcgg ttctggtgat gtccttcctg gtcactatgc
     7921 tgttcaactc caaactctct gacgatccga tttatcgcaa gcgtctggaa gaaggcctgg
     7981 ttgaactgcg cggtgaaaag cagattgaaa tcaaatccgg tgcaaaaacg tccgtctggc
     8041 tgttcctgct gggcgtagtt ggcgttgtta tctatgcaat catcaacagc ccaagcatgg
     8101 gtctggttga aaaaccgctg atgaacacca ccaacgcaat cctgatcatc atgctcagcg
     8161 ttgcaactct gaccaccgtt atctgtaaag tcgataccga caacatcctc aactccagca
     8221 ccttcaaagc aggtatgagc gcctgtattt gtatcctggg tgttgcgtgg ctgggcgata
     8281 ctttcgtttc caacaacatc gactggatca aagataccgc tggtgaagtg attcagggtc
     8341 atccgtggct gctggccgtc atcttcttct ttgcttctgc tctgctgtac tctcaggctg
     8401 caaccgcaaa agcactgatg ccgatggctc tggcactgaa cgtttctccg ctgaccgctg
     8461 ttgcttcttt cgctgcggtg tctggtctgt tcattctgcc gacctacccg acgctggttg
     8521 ctgcggtaca gatggatgac acgggtacta cccgtatcgg taaattcgtc ttcaaccatc
     8581 cgttcttcat cccgggtact ctgggtgttg ccctggccgt ttgcttcggc ttcgtgctgg
     8641 gtagcttcat gctgtaatga tcaatcgcgg ggcgttcacg ccccgctttc tttcccgccg
     8701 actaacatcc ttcccccgtc cgttgtatag tgacctctct cttgcggttc catctgttct
     8761 tgcgaggtgt ttatgcttga tgaaaaaagt tcgaataccg cgtctgtcgt ggtgctatgt
     8821 accgcaccgg atgaagcgac agcccaggat ttagccgcca aagtgctggc ggaaaaactg
     8881 gcggcctgcg cgaccttgat ccccggcgct acttctctct attactggga aggtaagctg
     8941 gagcaagaat acgaagtgca gatgatttta aaaactaccg tatctcacca gcaggcactg
     9001 ctggaatgcc tgaagtctca tcatccatat caaaccccgg aacttctggt tttacctgtt
     9061 acacacggag acacagatta cctctcatgg ctcaacgcat ctttacgctg atcctgctac
     9121 tttgcagcac ttccgttttt gccggattat tcgacgcgcc gggacgttca caatttgtcc
     9181 ccgcggatca agcctttgct tttgattttc agcaaaacca acatgacctg aatctgacct
     9241 ggcagatcaa agacggttat tacctctacc gtaaacagat ccgcattacg ccggaacacg
     9301 cgaaaattgc caacgtgcag ctgccacaag gcgtctggca tgaagatgag ttttacggca
     9361 aaagcgagat ttaccgcgat cggctgacgc ttcccgtaac catcaaccag gcgagtgcgg
     9421 gagcgacgtt aactgtcacc taccagggct gtgctgatgc cggtttctgt tatccgccag
     9481 aaaccaaaac cgttccgtta agcgaagtgg tcgccaacaa cgcagcgtca cagcctgtgt
     9541 ctgttccgca gcaagagcag cccaccgcgc aattgccctt ttccgcgctc tgggcgttgt
     9601 tgatcggtat tggtatcgcc tttacgccat gcgtgctgcc aatgtaccca ctgatttctg
     9661 gtatcgtgct gggtggtaaa cagcggctct ccactgccag agcattgttg ctgaccttta
     9721 tttatgtgca ggggatggca ctgacttaca cggcgctggg tctggtagtt gccgccgcag
     9781 ggttacagtt ccaggcggcg ctacagcacc catacgtgct cattggcctc gccatcgtct
     9841 ttaccttgct ggcgatgtca atgtttggtt tgtttaccct gcaactcccc tcttcgctgc
     9901 aaacgcgcct cacgctgatg agcaatcgcc aacagggcgg ctcacctggc ggtgtgtttg
     9961 ttatgggagc gattgccgga ctgatctgtt caccatgcac caccgcaccg cttagcgcga
    10021 ttctgttgta tatcgcccaa agcgggaaca tgtggctggg gggcggcacg ctttatctct
    10081 atgcgttggg catgggcctg ccgctgatgc taataaccgt ctttggtaac cgcctgctgc
    10141 cgaaaagcgg cccgtggatg gaacaagtca aaaccgcgtt tggttttgtg atcctcgcac
    10201 tgccggtctt cctgctggag cgagtgattg gcgatatatg gggattacgc ttgtggtcgg
    10261 cgcttggtgt cgcattcttt ggctgggcct ttatcaccag cctacaggcc aaacgcggct
    10321 ggatgcgcgt ggtgcaaata atcctgctgg cagcggcatt ggttagcgtg cgcccacttc
    10381 aggattgggc atttggtgca acacataccg cgcaaactca gacgcatctc aactttacac
    10441 aaatcaaaac ggtagatgag ttaaatcagg cgctcgttga agccaaaggc aaaccagtga
    10501 tgttagatct ctatgccgac tggtgcgtcg cctgtaaaga gtttgagaaa tacaccttca
    10561 gcgacccgca ggtgcaaaaa gcgttagcag acacggtctt acttcaggct aacgtaactg
    10621 ccaacgacgc acaagatgtg gcgctgttaa agcatcttaa tgtccttggc ctaccgacaa
    10681 ttctcttttt tgacggacaa ggccaggagc atccacaagc acgcgtcacg ggctttatgg
    10741 atgctgaaac cttcagcgca catttgcgcg atcgccaacc gtgaacgaca cttgcagtgg
    10801 gaaagacgga ggaaaatagc gtgcaacgtg aagatgtact gggagaagcc ctgaaattat
    10861 tagaattaca agggattgcc aacaccacgc ttgagatggt tgctgaacgt gtggattacc
    10921 cactggacga gctacgccgc ttctggccag acaaagaggc aatcctctac gatgcgctgc
    10981 gttatcttag ccaacagata gatgcctggc gtcgtcagct gatgctggac gaaacgcaaa
    11041 ccgccgaaca aaagctgctg gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc
    11101 gctatccggg ctgtctattt atcgctgcct gtacgtttta tcccgatcct ggccacccta
    11161 ttcatcaact ggccgatcag caaaaaagcg cggcctacga tttcacccac gaactgttaa
    11221 ccacactgga agttgacgat ccggcgatgg tagcaaagca gatggaactg gtgctggaag
    11281 gctgtttaag ccgaatgctg gtgaatcgta gccaggcgga tgtcgacacc gcacatcggc
    11341 tggcggaaga tatcctgcgc ttcgcccgct gccgccaggg tggtgctttg acctgacaga
    11401 aacacagaaa agaagcgatt tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt
    11461 aggttgacga gatgtgcaga ttacggttta atgcgccccg ttgcccggat agctcagtcg
    11521 gtagagcagg ggattgaaaa tccccgtgtc ggtggttcga ttccgcctcc gggcaccata
    11581 taccagaaca cgctggttaa gggatgagat ccttgacggg cggttaatac aggatgcggc
    11641 atcaaagggt ctccgtttgt gtaagttgtg gttacactga ttgagttcgg tatcgtatcc
    11701 tcaaacaggg tgtcttttgc ggagacgccc tgttctcgtt aacactgtat gcttacatcg
    11761 ctgattcagc ctgtcaagat ttattccccc ctttgcaaat cttgctcact gtgcgctcat
    11821 ctgtatacaa actcagggca tcttccagtg tctg
//
LOCUS       DAZGHF010000140        11795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGHF010000140 DAZGHF010000000
VERSION     DAZGHF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.141"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(137..931)
                     /gene="espL1"
                     /locus_tag="ACHK3A_004086"
                     /pseudo
     CDS             complement(137..931)
                     /gene="espL1"
                     /locus_tag="ACHK3A_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1454..3382
                     /gene="thrS"
                     /locus_tag="ACHK3A_004087"
     CDS             1454..3382
                     /gene="thrS"
                     /locus_tag="ACHK3A_004087"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO8742101.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            3386..3928
                     /gene="infC"
                     /locus_tag="ACHK3A_004088"
     CDS             3386..3928
                     /gene="infC"
                     /locus_tag="ACHK3A_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO8742102.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4025..4222
                     /gene="rpmI"
                     /locus_tag="ACHK3A_004089"
     CDS             4025..4222
                     /gene="rpmI"
                     /locus_tag="ACHK3A_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO8742103.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            4276..4632
                     /gene="rplT"
                     /locus_tag="ACHK3A_004090"
     CDS             4276..4632
                     /gene="rplT"
                     /locus_tag="ACHK3A_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO8742104.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4749..4873
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4755..4799
                     /gene="pheM"
                     /locus_tag="ACHK3A_004091"
     CDS             4755..4799
                     /gene="pheM"
                     /locus_tag="ACHK3A_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO8742105.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5083..6066
                     /gene="pheS"
                     /locus_tag="ACHK3A_004092"
     CDS             5083..6066
                     /gene="pheS"
                     /locus_tag="ACHK3A_004092"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO8742106.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6081..8468
                     /gene="pheT"
                     /locus_tag="ACHK3A_004093"
     CDS             6081..8468
                     /gene="pheT"
                     /locus_tag="ACHK3A_004093"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO8742107.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVIECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGAAIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8473..8772
                     /gene="ihfA"
                     /locus_tag="ACHK3A_004094"
     CDS             8473..8772
                     /gene="ihfA"
                     /locus_tag="ACHK3A_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO8742108.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8873..9853
                     /gene="btuC"
                     /locus_tag="ACHK3A_004095"
     CDS             8873..9853
                     /gene="btuC"
                     /locus_tag="ACHK3A_004095"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO8742109.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9916..10467
                     /gene="btuE"
                     /locus_tag="ACHK3A_004096"
     CDS             9916..10467
                     /gene="btuE"
                     /locus_tag="ACHK3A_004096"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO8742110.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            10467..11216
                     /gene="btuD"
                     /locus_tag="ACHK3A_004097"
     CDS             10467..11216
                     /gene="btuD"
                     /locus_tag="ACHK3A_004097"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO8742111.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQHQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLFLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            11295..11759
                     /gene="nlpC"
                     /locus_tag="ACHK3A_004098"
     CDS             11295..11759
                     /gene="nlpC"
                     /locus_tag="ACHK3A_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO8742112.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         2992 a         2722 c         3157 g         2924 t
ORIGIN      
        1 tgaatggcta tatgaacagc tgacatgttg tcatgatttt tagcggccaa cagtgtaaat
       61 aactgacgtt ggctaaaaga atattttttt caaaagtacc caacgttgat atataagata
      121 acacgacatc tttacttact gacaaagtat gattgtctat atataaaatc gcggacatat
      181 gactgacatt gtttgccatc attcgacaga agaaaatatc taaagcgaag ccaaaatcct
      241 tactcgcgat aacgcgactc cctgcaggag gatggcgaag taatgatctg aactccgcgt
      301 cccggactaa gggaacatgc tgctgtatgg attcaacgga ggagtaatgt ttaatcagat
      361 tgactttgcc ttttccattt ctcagtgaat caagggcgta ttgcaatgac agatggcgac
      421 aaaaaatagc gtcagaagga gattgcgaaa aaaatgcact accattgaga tttactgttg
      481 gttgtatagt tttttctcta tggatttttt tagacagttt catacaatgg ttacgatttt
      541 cggtagttaa ctgttcctgt tcaatataat ccagtatgct tcgcaaatgg ttagctttaa
      601 taggcgtggt ttccagtaat aaggaaatga tttctttttt atagtgtaat gtaatgagta
      661 tattttctgc atggatgggt gaagtggcaa agttaatggt aaaaagggca gtggaataac
      721 aattttgtga tatttttcga cagtttgcgg taaattcctg agtaacacag tatgacagca
      781 ttgaacttaa gtcagaggta tcaagtaggg tgatgagttg gcacgcaaat tgattaatag
      841 gctctgatga aggcgttggt atgtcgctat cgatgtgcat tctatgagga gacttataat
      901 gagttctgat aataatgtcg ttttgactca taatctattc gccttatata tgtctggagg
      961 ttttgaggat gatgattaat tattatcgcc agaacgaaat gtgcacgaaa aattgctata
     1021 agaattgtgt cacttttatc attgtgatat gaccacttcg tcattaatct tatgatcgtt
     1081 taaatagtac cgccaaatta cgcaacgaac tttgctggat tcagtttatg ctgctgcaaa
     1141 tccgctcgca gtaaaccttt cagacgctcc gtgatgttat cagtggttct ttaagcgttt
     1201 tgctggtgta ctcactacaa acgaattgcg aatcgaatca atgtgaaacg gaaaggtaca
     1261 atctcccttt ttttagtgtt aacatcgttc aaccgggtgg aaggtgagag gttaatgtct
     1321 ttcatttgtg tacctgattg cgaaccaatt tagcatttgt tggctagatg gtttcgcaat
     1381 gaactgttaa taaacaaatt tttctttgta tgtgatcttt cgtgtgggtc accactgcaa
     1441 ataaggatat aaaatgcctg ttataactct tcctgatggc agccaacgcc attacgacca
     1501 cgctgtaagc cccatggatg ttgcgctgga cattggtcca ggtctggcga aagcctgtat
     1561 cgcagggcgc gttaatggcg aactggttga tgcttgcgat ctgattgaaa acgacgcaca
     1621 actgtcgatc attaccgcca aagacgaaga aggtctggag atcattcgtc actcctgtgc
     1681 gcacctgtta gggcacgcga ttaaacaact ttggccgcat accaaaatgg caatcggccc
     1741 ggttattgac aacggttttt attacgacgt tgatcttgac cgcacgttaa cccaggaaga
     1801 tgtcgaagca ctcgagaagc ggatgcatga gcttgctgag aaaaactacg acgtcattaa
     1861 gaagaaagtc agctggcacg aagcgcgtga aactttcgcc aaccgtggtg agagctacaa
     1921 agtctccatt cttgacgaaa acatcgccca tgatgacaag ccaggtctgt acttccatga
     1981 agaatatgtc gatatgtgcc gcggtccgca cgtaccgaac atgcgtttct gccatcattt
     2041 caaactaatg aaaacggcag gggcttactg gcgtggcgac agcaacaaca aaatgttgca
     2101 acgtatttac ggtacggcgt gggcagacaa aaaagcgctt aacgcttacc tgcagcgcct
     2161 ggaagaagcc gcgaaacgcg accaccgtaa aatcggtaaa cagctcgacc tgtaccatat
     2221 gcaggaagaa gcaccgggta tggtattctg gcacaacgat ggctggacca tcttccgtga
     2281 actggaagtg tttgttcgtt ctaaactgaa agagtaccag tatcaggaag ttaaaggtcc
     2341 gttcatgatg gaccgtgtcc tgtgggaaaa aaccggtcac tgggacaact acaaagatgc
     2401 aatgttcacc acgtcttctg agaaccgtga atactgcatt aagccgatga actgcccggg
     2461 tcacgtacaa attttcaacc aggggctgaa gtcgtatcgc gatttgccgc tgcgtatggc
     2521 cgagtttggt agctgccacc gtaacgagcc gtcaggttcg ctgcatggcc tgatgcgtgt
     2581 acgtggtttt acccaggatg acgcgcatat cttctgtact gaagaacaaa ttcgcgatga
     2641 agttaacgga tgtatccgtt tagtctatga tatgtacagc acttttggct tcgagaagat
     2701 cgtcgtcaaa ctctccactc gtcctgaaaa acgtattggc agcgacgaaa tgtgggatcg
     2761 tgctgaggcg gacctggcag ttgcgctgga agaaaacaac atcccgtttg aatatcaact
     2821 gggtgaaggc gctttctacg gtccgaaaat tgaatttacc ctgtatgact gcctcgatcg
     2881 tgcatggcag tgcggtacag tacagctgga cttctccttg ccgtctcgtc tgagcgcctc
     2941 ctatgtgggc gaagacaacg agcgtaaggt accggtaatg attcaccgcg caattcttgg
     3001 gtcgatggaa cgtttcatcg gtatcctgac cgaagaattc gctggtttct tcccgacctg
     3061 gcttgcgccg gttcaggttg ttatcatgaa tattaccgat tcacagtctg attacgttaa
     3121 cgaattgacg caaaaactat caaatgcggg cattcgtgtt aaagcagact tgagaaatga
     3181 gaagattggc tttaaaatcc gcgagcacac tttgcgtcgc gtcccgtata tgctggtctg
     3241 tggtgataaa gaggtggaat caggcaaagt tgccgttcgc acccgccgtg gtaaagacct
     3301 gggaagcatg gacgtaaatg aagtgatcga gaagctgcaa caagagattc gcagccgcag
     3361 tcttaaacaa ttggaggaat aaggtattaa aggcggaaaa cgagttcaaa cggcgcgccc
     3421 taaccgtatc aatggcgaaa ttcgcgccca ggaagttcgc ttaacaggtc tggaaggcga
     3481 gcagcttggt attgtgagtc tgagagaagc tctggagaaa gcagaagaag ccggagtaga
     3541 cttagtcgag atcagcccta acgccgagcc gccggtttgt cgtataatgg attacggcaa
     3601 attcctctat gaaaagagca agtcttctaa ggaacagaag aaaaagcaaa aagttatcca
     3661 ggttaaggaa attaaattcc gtcctggtac agatgaaggc gactatcagg taaaactccg
     3721 cagcctgatt cgctttctcg aagagggtga taaagccaaa atcacgctgc gtttccgcgg
     3781 tcgtgagatg gcgcaccagc aaatcggtat ggaagtgctt aatcgcgtga aagacgattt
     3841 gcaagaactg gcagtggtcg aatccttccc aacgaagatc gaaggccgcc agatgatcat
     3901 ggtgctcgct cctaagaaga aacagtaagg ccttcaagta gcactgtctg tggagccttt
     3961 gggcttcaca ggcattgttc gcctttgttt cgtttattaa caatgcgaag tggaagttat
     4021 taaaatgcca aaaattaaga ccgtacgcgg tgctgctaag cgcttcaaaa aaaccggtaa
     4081 aggtggtttt aagcataagc acgctaacct gcgtcacatt ctgaccaaaa aagcgaccaa
     4141 acgtaaacgt cacctgcgtc cgaaagccat ggtttccaaa ggcgatctgg gcctggtaat
     4201 cgcgtgcctg ccgtacgcat aagccgttaa cgttttttaa ctttttaatt agaatataga
     4261 tacaggagag cacatatggc tcgcgtaaaa cgtggtgtta ttgcacgcgc acgtcacaag
     4321 aaaattttga aacaagctaa aggctactac ggtgcgcgtt ctcgcgtata ccgcgttgcc
     4381 ttccaggctg ttatcaaagc tggtcagtat gcttaccgtg accgtcgtca acgtaagcgt
     4441 cagttccgtc aactgtggat tgcgcgtatc aacgcagcag cacgtcagaa cggtatttct
     4501 tacagcaaat tcatcaatgg cctgaaaaaa gcctctgttg aaatcgaccg taagatcctg
     4561 gctgatatcg cagtattcga caaagtagcg ttcaccgctc tggttgaaaa agcgaaagca
     4621 gctctggcat aagccagttg aaagagggag ctagtctccc tcttttcgtt tcaacgccat
     4681 caaaacattg acttttatcg ccgtagcctt ttcaataaag gtcttttgaa gagtaccaaa
     4741 aggtaacgca agcaatgaat gctgctattt tccgcttctt tttttacttt agcacctgac
     4801 tccaggaggc tagcgcgtga gaagagaaac ggaaaacagc gcctgaaagc ctcccagtgg
     4861 aggctttttt tgtatgcgcg ttttgaaaaa taaaaagcct gtctcatcag gcgtaattgt
     4921 cgcagccagt ctggacacgg ccagcgcgga gaaaccggag cgtacttaag tacgtgagga
     4981 tttcgagcac tgcccgggac caaaatggca agtaaaatag cctgatggga tgggctctaa
     5041 gtccaacgaa ccagtgtcac cactgacaca atgaggaaaa acatgtcaca tctcgcagaa
     5101 ctggttgcca gtgcgaaggc ggccattagc caggcgtcag atgttgccgc gttagataat
     5161 gtgcgcgtcg aatatttggg taaaaaaggg cacttaaccc ttcagatgac gaccctgcgt
     5221 gagctgccgc cagaagagcg tccggcagct ggtgcggtta tcaacgaagc gaaagagcag
     5281 gttcagcagg cgctgaatgc gcgtaaagcg gaactggaaa gcgctgcact gaatgcgcgt
     5341 ctggcggcgg aaacgattga tgtctccctg ccgggtcgtc gcattgaaaa cggcgggctg
     5401 catccggtga cccgtaccat cgaccgtatc gaaagtttct tcggtgagct tggctttacc
     5461 gtggcaaccg ggccggaaat cgaagacgat tatcataact tcgatgctct gaacattcct
     5521 ggtcaccacc cggcgcgcgc tgaccacgac actttctggt ttgacgctac ccgcctgctg
     5581 cgtacccaga cctctggcgt acagatccgc accatgaaag ctcagcagcc accgattcgt
     5641 atcatcgcgc ctggccgcgt ttatcgtaac gactacgacc agactcacac gccgatgttc
     5701 catcagatgg aaggtctgat tgttgatacc aacatcagct ttaccaacct gaaaggcacg
     5761 ctgcacgact tcctgcgtaa cttctttgag gaagatttgc agattcgctt ccgtccttcc
     5821 tacttcccgt ttaccgaacc ttctgcagaa gtggacgtca tgggtaaaaa cggtaaatgg
     5881 ctggaagtac tgggctgcgg gatggtgcat ccgaacgtgc tgcgtaacgt tggcatcgac
     5941 ccggaagttt actctggttt cgccttcggg atggggatgg agcgtctgac catgttgcgt
     6001 tacggcgtca ccgacctgcg ttcattcttc gaaaacgatc tgcgtttcct caaacagttt
     6061 aaataaggca ggaatagatt atgaaattca gtgaactgtg gttacgcgaa tgggtgaacc
     6121 cggcgattga tagcgatgcg ctggcaaatc aaatcactat ggcgggcctg gaagttgacg
     6181 gtgtagaacc ggttgccggt agcttccacg gcgtggtcgt tggtgaagtg attgagtgtg
     6241 cgcagcatcc gaacgctgac aaactgcgtg tgacaaaagt gaatgtcggc ggcgatcgcc
     6301 tgctggacat cgtctgcggt gcgccaaact gccgtcaggg cctgcgtgtg gcggtagcga
     6361 ccattggtgc tgttctgccg ggcgatttca aaattaaagc ggcgaaactg cgtggcgaac
     6421 cgtctgaagg gatgctgtgc tccttttctg agctgggaat ttctgacgat cacagcggca
     6481 ttatcgagct gcctgcagat gcgccgattg gcaccgacat ccgcgaatac ctgaaactcg
     6541 atgacaacac catcgaaatc agcgtaacgc caaaccgtgc cgattgctta ggcattatcg
     6601 gcgtggcgcg tgatgttgcc gtgctgaacc agctgccgct ggttgaaccg gaaatcgttc
     6661 cggttggtgc ggccatcgac gacacgctgc cgattacagt cgaagcgccg gaagcctgcc
     6721 cgcgctatct tggccgtgtg gtaaaaggca ttaacgttaa agcgccaact ccgctgtgga
     6781 tgaaagaaaa actgcgtcgt tgcgggatcc gttctatcga tgcagttgtt gacgtcacca
     6841 actatgtgct gctcgaactg ggccagccga tgcacgcttt cgataaagat cgcattgaag
     6901 gcggcattgt ggtgcggatg gcgaaagagg gcgaaactct ggtgctgctt gatggcactg
     6961 aagcgaagct gaatgctgac accctggtca tcgccgacca caacaaggcg ctggcgatgg
     7021 gcggcatctt tggtggcgaa cactctggcg tgaatgacga aacgcaaaac gtcctgctgg
     7081 aatgcgcttt cttcagcccg ctgtctatca ccggtcgtgc tcgtcgtcat ggcctgcata
     7141 ctgatgcgtc tcaccgttat gagcgtggcg ttgatccggc actgcagcac aaagcgatgg
     7201 aacgtgcgac ccgtctgctg atcgacatct gcggtggtga ggctggtccg gtaattgata
     7261 tcaccaacga agcaacgctg ccgaagcgtg ccaccattac tttacgtcgc agcaaactgg
     7321 atcgcctgat cggccatcat attgctgatg agcaggtaac tgacattctg cgtcgtctcg
     7381 gctgcgaagt gaccgaaggc aaagacgaat ggcaggccgt tgcgccgagc tggcgtttcg
     7441 acatggagat tgaagaagat ctggtcgaag aagtcgcgcg tgtttacggc tacaacaaca
     7501 tcccggatga gccggtacag gcaagcctga ttatgggtac tcaccgcgaa gccgatctgt
     7561 cgctcaagcg cgtgaaaacg ctgctcaacg acaaaggcta tcaggaagtg atcacctaca
     7621 gcttcgttga tccgaaagtg cagcagatga tccatccagg cgttgaagcc ttactgctgc
     7681 caagccctat ttccgttgaa atgtcagcaa tgcgtctttc cctgtggacc ggcctgctgg
     7741 caaccgtggt gtacaaccag aaccgtcagc agaaccgtgt gcgcattttc gaaagtggtc
     7801 tgcgtttcgt accagatact caggcaccgt tgggcattcg tcaggatctg atgttagccg
     7861 gtgtgatttg cggtaaccgt tacgaagagc actggaacct ggcaaaagag accgttgatt
     7921 tctatgattt gaaaggcgat cttgaatccg ttctcgacct gaccggtaaa ctgaatgagg
     7981 ttgagttccg tgcagaagcg aatccggcac tgcatccggg gcaatccgca gcgatttatc
     8041 tgaaaggtga acgtattggt tttgttgggg ttgttcatcc tgaactggaa cgtaaactgg
     8101 atcttaacgg tcgcactctg gtgttcgaac tggagtggaa caagctcgca gaccgcgtgg
     8161 tgcctcaggc gcgcgagatt tctcgcttcc cggcgaaccg tcgtgacatc gcggtggtgg
     8221 tcgcagaaaa cgttcccgca gcggatattt tatccgaatg taagaaagtt ggcgtaaatc
     8281 aggtagttgg cgtaaactta tttgacgtgt accgcggtaa gggtgttgcg gaggggtata
     8341 agagcctcgc cataagcctg atcctgcaag ataccagccg tacactcgaa gaagaggaga
     8401 ttgccgctac cgtcgccaaa tgtgtagagg cattaaaaga gcgattccag gcatcattga
     8461 gggattgaac ctatggcgct tacaaaagct gaaatgtcag aatatctgtt tgataagctt
     8521 gggcttagca agcgggatgc caaagaactg gttgaactgt ttttcgaaga gatccgtcgc
     8581 gctctggaaa acggcgaaca ggtgaaactc tctggttttg gtaacttcga tctgcgtgat
     8641 aagaatcaac gcccgggacg taacccgaaa acgggcgagg atattcccat tacagcacgg
     8701 cgcgtggtga ccttcagacc cgggcagaag ttaaaaagcc gggtcgaaaa cgcttcgccc
     8761 aaagacgagt aatctgatct aactaaaaag gccgctctgc ggcctttttt cttttcactg
     8821 tcgaagagtc accgtaaaat caacgccatg acacttcagc agaacggata ccatgctgac
     8881 acttgcccgc caacaacagc gacaaaatat tcgctggtta ttatgcctgt cagttttgat
     8941 gctgctggcg cttctcttaa gcctttgcgc cggtgaacta tggatctcgc caggtgactg
     9001 gttttctcct cgtggcgaac tgttcgtctg gcaaattcgc ctgccacgta cgctggctgt
     9061 attgctggtt ggtgcggcgc tggctatatc cggcgctgta atgcaggcgt tgtttgaaaa
     9121 tcctctggca gaacctggac tacttggcgt ctctaacggc gcaggcgtgg ggcttatcgc
     9181 cgcggtattg cttgggcaag ggcaactccc caactgggcg ctagggctgt gtgcgattgc
     9241 tggcgcgctt atcatcactt taatactctt acgtttcgct cgtcgtcatc tttcgaccag
     9301 tcggttattg ctggctggcg ttgcattagg gattatctgt agcgcactaa tgacgtgggc
     9361 tatctacttt tccacctctg ttgatttacg tcagctgatg tactggatga tgggcggttt
     9421 tggcggcgta gactggcggc aaagctggct gatgctggca ttgatccccg tgttgttgtg
     9481 gatctgttgt cagtccaggc cgatgaatat gttagcactt ggcgagatct cggcgcggca
     9541 actgggttta cccctgtggt tctggcgcaa tgtgttggtc gcagcgaccg gctggatggt
     9601 tggcgtcagt gtggcgctgg cgggtgctat cggctttatt ggtctggtga tcccacatat
     9661 tctccggctg tgtggtttaa ccgatcatcg cgtattactt cccggctgcg cgctggcagg
     9721 ggcgagcgca ttgctgctgg ccgatattgt agcgcgcctg gcattagctg ccgcagagct
     9781 gcctattggc gtggtcaccg caacgttggg tgcgccggtg tttatctggt tattgttaaa
     9841 agcaggacgt tagccgcaaa aagacggtct atgattaaaa gctaagattt ttactgacca
     9901 cacccaggag aaacgatgca agattccatt ctgacgaccg tagtgaaaga tatcgacggt
     9961 gaagtgacca cgctggagaa gtacgccggt aatgtgctgt tgattgtcaa tgtcgcctca
    10021 aagtgtggct taacgccgca atatgagcag ttggagaata ttcagaaagc ctgggccgat
    10081 cgtggttttg tggtgctggg attcccgtgc aaccagtttc tggaacaaga accgggcagc
    10141 gatgaagaga ttaaaactta ctgtaccacc acatgggggg tgacgttccc gatgttcagt
    10201 aagattgaag ttaatggcga aggacgccat ccgctgtatc aaaaattgat tgccgcagcg
    10261 ccgaccgcag tcgcgccgga agagagcgga ttctatgctc gtatggtcag caaaggtcgt
    10321 gcaccgctgt accctgatga tattttatgg aattttgaga aatttttagt tggcagggac
    10381 ggattggtca tccagcgttt ttccccggat atgacgccgg aagatcccat tgtgatggaa
    10441 agcattaaac tggcgttggc aaaataatgt ctattgtgat gcagttacag gatgttgcgg
    10501 aatctacccg cctggggccg ctttctggcg aggttcgggc tggggagatc ctgcacctgg
    10561 tggggccgaa tggcgcgggt aagagtacct tactggcgcg aatggccgga atgaccagcg
    10621 gtaagggaag cattcagttc gcggggcaac cactggaagc atggtccgca acaaaactcg
    10681 cgctacatcg cgcctatctt tcacaacacc agacgccacc gtttgcaatg ccggtctggc
    10741 actacctgac actgcatcag cacgataaaa cgcgtaccga actactgaat gatgtcgcag
    10801 gggcgctggc tcttgatgac aaactcggac ggagcaccaa tcaactttcc ggcggtgaat
    10861 ggcaacgcgt acgtcttgct gcggtggtgt tgcaaatcac accacaagcc aatcccgcag
    10921 gccaattgct gtttcttgat gagccgatga acagtcttga tgttgcgcaa caaagtgcgt
    10981 tagacaaaat tctgagcgca ctgtgtcagc aaggactggc gattgtgatg agcagtcacg
    11041 atctcaacca caccttgcgt catgcgcatc gggcgtggtt gctaaaaggt ggaaaaatgc
    11101 tggccagtgg acgcagggaa gaggtgctca cgccgccaaa tctggcgcag gcctatggga
    11161 tgaattttcg ccgtctggat atcgaaggtc acagaatgct gatttcgacc atctgatttc
    11221 attaatatct tgaaaatcgg gtaattacga cgctaaatta atgcaagaat aaaaaaacag
    11281 aggaaccgta cggaatgcgt ttctgcctta ttttgatcac agcactgttt ctggccgggt
    11341 gtagccacca taaagcaccg ccgccaaatg ccagactttc tgattcgatt accgttattg
    11401 ccggtttgaa cgaccagcta caaagctggc atggcacgcc gtatcgttat ggtggcatga
    11461 cgcggcgcgg tgtggactgt tcggggtttg tggttgtgac gatgcgcgat cgtttcgatt
    11521 tgcagctgcc ccgtgaaacc aaacaacaag cctctatcgg cacgcaaatt gataaagacg
    11581 agttgctgcc tggtgacctg gtctttttca aaacgggctc cggacaaaat ggtttgcatg
    11641 taggtattta tgataccaac aaccaattta tccacgcctc taccagcaag ggagtgatgc
    11701 gttcctcact tgataatgtc tattggcaga aaaatttctg gcaggcgaga cgaatctagt
    11761 atgtcgaaaa atggacggcg atgatacatc gccgt
//
LOCUS       DAZGHF010000141        11666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGHF010000141 DAZGHF010000000
VERSION     DAZGHF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.142"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..234
                     /locus_tag="ACHK3A_004099"
     CDS             <1..234
                     /locus_tag="ACHK3A_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein acyltransferase"
                     /protein_id="HFO8742113.1"
                     /translation="HNGVVITQSDYIRLNNGRGYKTTLVNGSLLAKFSVAPNTKLINV
                     QNYGGTIVFNNDWEIDWDIYNKSKKEHFFVVTL"
     gene            complement(382..1317)
                     /locus_tag="ACHK3A_004100"
                     /pseudo
     CDS             complement(382..1317)
                     /locus_tag="ACHK3A_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1661..2281)
                     /gene="entD"
                     /locus_tag="ACHK3A_004101"
     CDS             complement(1661..2281)
                     /gene="entD"
                     /locus_tag="ACHK3A_004101"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO8742114.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFEPASFHEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAVHALREFGFKYAPPVGERRQPLWPAGLYGSISHCQATALAVVSHQ
                     PTGIDIEEIFSAQAAEELKDSIMTPAEYELLVVSNLPFSVVLTLAFSAKESAFKASKL
                     QTDQGFLDYQIVNWNKQRIIICWENEIFAVQWQIRGKQLITLCQHD"
     gene            complement(2435..3783)
                     /locus_tag="ACHK3A_004102"
     CDS             complement(join(2435..3303,3303..3783))
                     /locus_tag="ACHK3A_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO8742115.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGPRALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNMVKNWIKGYRK
                     SGNEAFIRRRKEKSMTRSDDTHENEANMTPEEMKNELRYLRAENAYLKAMQEHLLEKK
                     PPGAGEKTKVIRSLKCGHCQSDLLKAAGLARSTLYYQLSLQKAKDKYADVKQLIASIF
                     HEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAE
                     NILQRQFKAEAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVK
                     RMLNKGLESLAEGEKPLLHNDQGWHYRIKSYQSALADKGLVQSMSRKGNCLDNAVMEN
                     FLGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPLEYRTEYQKAG"
     gene            complement(3893..6133)
                     /gene="fepA"
                     /locus_tag="ACHK3A_004103"
     CDS             complement(3893..6133)
                     /gene="fepA"
                     /locus_tag="ACHK3A_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO8742116.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            6376..7578
                     /gene="fes"
                     /locus_tag="ACHK3A_004104"
     CDS             6376..7578
                     /gene="fes"
                     /locus_tag="ACHK3A_004104"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO8742117.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFRWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMQANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            7581..7799
                     /gene="ybdZ"
                     /locus_tag="ACHK3A_004105"
     CDS             7581..7799
                     /gene="ybdZ"
                     /locus_tag="ACHK3A_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO8742118.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQVSCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            7796..>11666
                     /gene="entF"
                     /locus_tag="ACHK3A_004106"
     CDS             7796..>11666
                     /gene="entF"
                     /locus_tag="ACHK3A_004106"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO8742119.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPETIDLRTNIDPHGTAQSLMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPHAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTSLCYNAPLTPQGSAPLKLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFVRQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DSQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERALQEGMNPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRAT"
BASE COUNT         2595 a         3064 c         3058 g         2949 t
ORIGIN      
        1 cacaacgggg tcgtaataac acaatctgat tacataagac ttaataacgg taggggatat
       61 aagactacat tggttaacgg tagcttactt gcaaagtttt ccgttgcacc aaacaccaag
      121 ttaataaatg tccagaatta tggtggaact attgttttta ataatgattg ggaaattgac
      181 tgggatattt ataacaaatc caaaaaagaa catttttttg tggttacttt ataatacagc
      241 gagggagtgt tgttgaaatt tttatgagct atttacgcgc ccacatatta ttcaagtctt
      301 gcatatatat gctgagtagt taaattcccc tgtgctatgc cagttactcc attcgcttct
      361 aagtagctga tcctaatacc gctacacttt agccagccca tgctttccac cggggaatgg
      421 gctgacggta tccataaagt gacgaacctt tttcaacagt tgccacattg aacggcactg
      481 gtgatttcgg gttattgttt catgaagcgc ctgccacaac cgttcgacat gattgaccca
      541 tggcgagtaa attggctgat aaatcaccct gaactttggg tttgctttca gccagcgcaa
      601 cgtttcacag cttttgtggg tgatgtagtt atcgacaatg agcgtaatct tttttgcccg
      661 tcagtatgtc tccttcagat gttttaacag cctgataaat aaggatgaac ctttactgtt
      721 tccacccacg tagctgactt tgcccgtacc actgtgcagc gcaccggcaa gatagtactt
      781 ttcattccgg cccggcgtaa caacgcgctt ttgctgcccg cgtaactgcc agtctgcgcc
      841 gattttggga ttgaggtgga tctccacttc atcttcgtaa aacacagggt gttctatgct
      901 acaactgact aacgctacat taatcgctgc cattttttct tccttattcg ggccccggat
      961 acgcagtgtt ggtgctgctc tgcgccatac cagttcggct gcaggtaacc atcgccggga
     1021 gaatgcttaa caagctcacg cagcaatgcg caaatatgct cgaacggcca gcgcctctca
     1081 cgtcctgacg gcaatgactt caggccttca acacccgaca gcgtgaacca gttaatccag
     1141 cgaccaatcg atgagcgagc acagcagagt gttctggcag catgactgac ggtgtcacca
     1201 cgatgcagca tcagcatggc tgtgagccgc cgggcgtggt tttttgtcct ttgttttatg
     1261 gatcgttttc tggatcaggc gtcgttcagt tctgggtatt ggtgctatga tcggcactgc
     1321 tccgtccggt tggtgatttg ggatattcag cgattgatca gatcgcataa tcgactctgt
     1381 aacaacctga acggtgtcgg ggtggtaaca taggttagga aacagggtgc ggatgccatc
     1441 atggcgcggg tcatcagtcc actaacgacg ccggatatat ggcagcaaat cagtggttgt
     1501 atattgaaca actataatag cgtcagatct caccattata acggtgggct gacaccgaaa
     1561 aaattagaga acagataacg ttcttagcgt aaaacggtgg tcaatattac ttgactacta
     1621 caagcaatgg ttgctggcta accacagtaa gggttatcca ttaatcgtgt tgacaaagcg
     1681 ttatgagttg ttttcctctt atctgccatt gcacagcaaa tatctcgttc tcccaacaaa
     1741 taatgatccg ctgtttattc cagttcacta tctgataatc cagaaaacct tgatctgtct
     1801 gtaacttgct tgccttaaac gcactctctt tcgctgaaaa cgccagtgta agcaccacag
     1861 aaaagggtaa attgctgacc acaagcagct cgtattctgc tggtgtcata atgctgtctt
     1921 tcaactcttc cgctgcttgt gcagagaaga tctcttcgat atcaataccc gttggttgat
     1981 gggataccac cgctaatgcc gtagcctggc agtgactaat actgccgtat aacccggcag
     2041 gccaaagtgg ctggcgacgt tcaccgacag ggggggcgta cttaaagcca aattcccgta
     2101 aagcatgaac agcagcaatc cgtccggcta aatgttcggc tttgcgtttc cgtccggcat
     2161 gttgcagttg cgcataatgc ggcagccaga gtaaatcctg ttcgtgaaaa cttgctggct
     2221 cgaactcaac aaaatgcagc gtatgtccgg caaaggggag ggaggtatgc gtagttttca
     2281 tatcgaccat attagagact gatgacaaac gcaaaactgc ctgatgcgct acgcttatca
     2341 ggcctacact atcctgcaat atattgaatt ggcaagattt ttgtaggccg gataaggcgt
     2401 tctgaactga accccaaaac ctggacagtt tcagttaacc ggctttttga tactcagtcc
     2461 ggtattctaa cgggctgagt cccccaagac tgagttttat tcttttttga ttccagtaag
     2521 ttatgtattc gttaacggca ttttccagct cttctacact tctgtagtcc cggcgatagt
     2581 acatttcttc tttcaggtga cctaagaaat tctccattac cgcattatca agacagttgc
     2641 ccttgcgtga catgctctgc actaaccctt tgtctgccag ggcagactga taacttttta
     2701 tccggtaatg ccacccttga tcattgtgga gcagcggttt ttctccttct gcaaggcttt
     2761 ccagcccctt atttaacatg cgcttcacca gctcctctgt aggcctgcag gcagtttccc
     2821 acgccacgat ttccccgtta aacaaatcaa gtatcgggga caggtacagt ttttgtccgc
     2881 cagccctgaa ttcggtgata tctgtcaccc atttctcaca gggagcttcc gctttgaact
     2941 gccgctgaag gatattttct gcagcgagtc ccatatttcc ccggtaagac cggtatttct
     3001 tcaggcgcac aggagacttc aggccgagct gttgcatcag ttttcgcacc gttttgccac
     3061 tgaacttaag acctcttttc tgaagctcac aatggatccg ccggtaccca taacatcccc
     3121 ggtgttcatg gaagatggag gctatgagct gtttcacatc agcatacttg tccttagcct
     3181 tctgcagtga cagctggtag tacagcgtgc ttctggccag ccctgcagcc tttaacaggt
     3241 cagactggca gtgtccgcac ttcaggctcc ggatgacttt cgttttttct ccagctcctg
     3301 gcgctttttt tccaggagat gctcctgcat ggctttcaga tatgcatttt ctgcgcgaag
     3361 ataacgcagc tcattcttca tctcttccgg cgtcatgttt gcctcgtttt catgggtatc
     3421 atcagaacgg gtcatgcttt tctccttcct gcgcctgata aaggcctcat taccagattt
     3481 gcggtagcct tttatccagt ttttaaccat gttgtggctg ggaatgttaa accgggcagc
     3541 tacccggggt agagactcag aatgcccgag agcataaaga accacgcaaa gtttatcttc
     3601 aggagaatag ctacgcttgt gtctgcaatc cagtgcccga ggaccgtgaa gaagaaaaag
     3661 attaatccag tgtgagaggc tggtgtgaga aagttgaaag agttttgccg tagaaatgat
     3721 gcctgcatga ccagccaggt aatgatttac agcggcaagt tttacttcaa atgaatgttt
     3781 cataaactgc accttcagat gtcagattat gtccaacatt tggggtgcag ttcagtttca
     3841 cgccgcatcc ggcatgaatt acgcgcactt tgtcaacaat ctgacgttag catcagaagt
     3901 gagtattaat gctcatatac cacgtacgtc ccggctcgtt ataggtatac gccccggcac
     3961 cggcgatata gttggctcct gccagatcgc ccgtggtctg ggcattaccc gcacgccaca
     4021 aacgtttgtc gaacaggttg tccacgccgc cggtcagact gacattcttc gtcacatccc
     4081 aggtcgcgct cagacccaca atgctgtaag gactgatttc tttggtttcc ggtccaaccg
     4141 ctggctgacc tttatagttg tacttcttcg gctgctgctt gccgtaccag gtgaaggttg
     4201 tttgcatcga caaatcttcc cgtgcctgcc agctcagcgt tgagttcaac gtatactccg
     4261 ggatgatcga caaacggtcg cccgtggttt tgttttcact cttcagcata taagtgatgt
     4321 tattggtcca catcaccgtt tcgctaaccg gtacgtttaa cgatccttcc agaccttcaa
     4381 ccaccgcttt cggtacgtta tcccactgat agagatcggt gccgactgcg ttttgcccta
     4441 cagccacata gcccgcttca atcttattgc gataatcgtt acggaaccag gtgacgcccg
     4501 ccagccaacc gtcgcgtttg aactccagac caatctcttt gttgatgctg gtttccgctt
     4561 tcaggtcatc attaccttgc agatagcagc cgcccgcgct ggcatagcag ccctgacctt
     4621 tactgtagag aatgtagttt gggttagtct ggtacagact cggcgcttta taagcgcgag
     4681 caatgcccat tttcagcgtg aagtcatcgc ctaaaccttg cgatatgttc agcgccggac
     4741 tccagttatt gccgacaata ctgtgatgat cgaaacgcag ccccggcgtt acgatggtgc
     4801 tgtcagttag ctccatattg ttttcggcaa acagcgagaa aatttctgct tttgaatacg
     4861 ggctacggtc ggtggcactc acgccatcaa tagcgccgcc ggtattcgtc ccggtgagcg
     4921 cttgggtgtt ggaactcaag tccttcatcc gctgctgatt ccactccgta cccagcgtca
     4981 gcgtctggtt aacgaggaaa tcaatcggca ggttaacttc gctgtgcagc atcacgtcat
     5041 cgagatcgat atcgacgaaa tcctgtgtcg ctttttcgtt aaatttccct tcggtaccgc
     5101 ccgccagacc ttccggaata cgcgagttac gggtgtgttc gtactgcacc cagttgctgg
     5161 tggtcacgcc gttatcccag ccgccgttcc atgtcagcga gtagttctgg cgatacagac
     5221 ggttggtttc atcgccatat ttcgagcggg tataggagtc agagttggtg ttctgggtat
     5281 cgcccgcata caggttaccc tggcggctgt aacccgcttc cagttccagc gattgcaatg
     5341 gtgcgaagtc ccagcgcacc acgccgttaa tatctttgtt gatcacccct tcgcgcccgg
     5401 ctggtaacgt cgtggcatac gttccggcac gcgcggactg atggccctgg ttgatatccc
     5461 acgcgtcagc ctgagttttg tcgaggttgc catacaagcg gaagctgaat tcgtcgccca
     5521 gcggaccggt gaggctaaag ttagtgcgtt tggtggcacc ttcctcttta tgttctggcg
     5581 cattgaaata agcgtcccac gagccgtgcc actcaccact gccttttttg gtaatgatgt
     5641 taaccacgcc gcccgccgcg ccgttgccat aacgcgcagc tgccggacca cgcagaactt
     5701 caatacgttc aatcatttca ggtggcaccc aggaggtatc accacgggta tcgcgctcgc
     5761 cacgccagcc ctgacgcacc gagttacggc tgcttaccgg cttgccgtca atcaaaatca
     5821 gcgtgttttc cggacccata ccgcgaatat caatctggcg gttattccca cgctgaccac
     5881 tggtggagtt accggtcagg ttaacgcctg gcatggtacg gatgatctcc gacacatcgc
     5941 gggcaaccgg gtttttgcgg atttcatctg cggtgatggt cgaaacgcca ggtgcctgca
     6001 agttctgctc ggcggcggta acgacaatgg tatcgtcatg tgaaacagga gtatcggtcg
     6061 gctcttgtgc ctgcgctacc ccataaatcc ccagattgac caacaaggcc agggaatgaa
     6121 tcttcttgtt cattgtttta ttcctgcatt tttgccacga attgcaactg tcgggcatgg
     6181 tcgtcatcaa cacgacgcat cccgctaccg cgaaaacctt tgatcctgaa agacacgcag
     6241 tgcagttggt taattaatgt ccgcgcttcc cacggcgcgc cattacgcta ttgcaaatgc
     6301 aaatagttat caataatatt atcaatatat ttctgcaatc aatgaaaaat tgcacagtaa
     6361 acatggggtt atggtgtgac ggcgttaaaa gtaggaagtg agagctggtg gcagtcgaaa
     6421 catggcccgg aatggcagcg tctgaatgac gaaatgtttg aggtcacttt ccggtggcgt
     6481 gatccccagg gttctgaaga atactcgacg ataaagcgcg tatgggtcta catcactggt
     6541 gtgaccgatc accatcagaa cagccagccc cagtcgatgc agcgaattgc aggcaccaac
     6601 gtctggcagt ggacgacaca acttaatgcc aactggcgcg gcagctactg ttttattccc
     6661 accgaacgcg atgacatttt ttctgcacca tcccccgatc gcctcgaatt acgtgaaggc
     6721 tggcgaaaac tattacccca ggcgatagcc gatccgctga atccacaaag ctggaaaggc
     6781 gggcgagggc acgctgtttc tgcactcgaa atgccgcaag cgcccctaca accgggatgg
     6841 gattgtccgc aagcgccaga aacacctgcc aaagaaatta tctggaaaag tgagcggttg
     6901 aaaaattcac gccgtgtatg gatttttacc actggcgatg caacagcagg agaacgtccg
     6961 ctggcagttt tgctcgatgg cgaattttgg gcgcagagta tgcctgtctg gcctgtgctg
     7021 acttcgttga cccatcgtca gcaacttcct ccagccgtgt atgtgttgat cgacgctatt
     7081 gacaccacgc accgcgccca cgaactgccg tgtaatgcgg atttctggct ggcagtacag
     7141 caagagttat tacccctggt gaaagctatt gcccctttta gcgatcgcgc cgatcgcacc
     7201 gtggtcgccg ggcagagttt tggtgggctt tccgcgctgt atgccggact gcactggcct
     7261 gaacgctttg gctgtgtatt aagccagtca ggatcgtact ggtggccgca tcggggcggg
     7321 cagcaagaag gtgttttact tgagcaactt aaagctggtc aagttagtgc cgaaggtctg
     7381 cgcattgtgc tggaagcggg tattcgcgag ccgatgatca tgcaggccaa tcaggcgctg
     7441 tatgcgcaat tacaccccat aaaagaatcc attttctggc gtcaggttga cggcggacat
     7501 gatgcgcttt gttggcgcgg tggcttgatg caggggctaa tcgacctctg gcaaccactt
     7561 ttccatgaca ggagttgaat atggcattca gtaatccctt cgatgatccg cagggagcgt
     7621 tttacatttt gcgcaatgcg caggggcaat tcagtctgtg gccgcaacaa tgcgccttac
     7681 cggcaggctg ggacgttgtg tgtcagccgc agtcacaggt gtcctgccag cagtggctgg
     7741 aagcccactg gcgtactctg acaccggcga atttcaccca gttgcaggag gcacaatgag
     7801 ccagcattta cctttggtcg ccgcacagcc cggcatctgg atggcagaaa aactgtcgga
     7861 tttaccctcc gcctggagcg tggcgcatta cgttgagtta accggagagg ttgatgcgcc
     7921 attactggcc cgcgcggtgg ttgccggatt agcgcaagca gatacgctgc ggatgcgttt
     7981 tacggaagat aacggcgaag tctggcagtg ggtcgatgat gcgctgacgt tcgaactgcc
     8041 agaaactatc gacctgcgaa ccaacattga tccgcacggt actgcgcagt cattaatgca
     8101 ggcggatttg caacaagatc tgcgcgtcga tagcggtaaa ccactggtct ttcatcagtt
     8161 gatacaggtg gcggataacc gctggtactg gtatcagcgt tatcaccatt tgctggtcga
     8221 tggcttcagt ttcccggcca ttacccgcca gatcgccaat atttactgcg catggctgcg
     8281 tggcgaacca acgcctgctt cgccgtttac gcctttcgct gatgtagtgg aagagtacca
     8341 gcaataccgc gaaagcgaag cctggcagcg tgatgcggca ttctgggcgg aacagcgtcg
     8401 tcaactgcca ccgcccgcgt cactttctcc ggcaccttta ccggggcgca gcgcttcggc
     8461 agatattttg cgcctgaaac tggaatttac cgacggggaa ttccgccagc tggctacgca
     8521 actttcaggt gtgcagcgta ccgatttagc ccttgcgctg acggctttgt ggctggggcg
     8581 attgtgcaac cgcatggact acgctgccgg atttatcttt atgcgtcgac tgggctcggc
     8641 ggcgctgacg gctaccggac ccgtgctcaa cgttttaccg ttgggtattc acattgcggc
     8701 gcaagaaacg ctgccgcaac tggcaacccg actggcagca caactgaaaa aaatgcgccg
     8761 ccatcaacgc tacgacgccg aacaaattgt ccgtgacagt gggcgagcgg caggagagga
     8821 accgctgttc ggtccggtac tcaatatcaa ggtatttgat taccaactgg atattcctgg
     8881 tgttcaggcg caaacccata ccctggcaac cggtccggtt aatgaccttg aactggccct
     8941 gttcccggat gaacacggag atttgagtat tgaaatcctc gccaataaac agcgttacgc
     9001 tgagccaacg ttaatccagc atgctgaacg cctgaaaatg ctgattgccc agttcgccgc
     9061 ggatcctgcg ctgttgtgcg gcgatgtcga tattatgctg ccaggtgaat atgcgcagct
     9121 ggcgcagatc aacgccactc aggttgagat tccagaaacc acgctgagcg cgctggtggc
     9181 agaacaagcg gcaaaaacac cgcatgctcc ggcgctggca gatgcgcgtt acctgttcag
     9241 ctatcgggaa atgcgcgagc aggtggtggc gctggcgaat ctgctgcgtg agcgcggcgt
     9301 taaaccaggc gacagcgtgg cggtggcgct accgcgctcg gtctttttga ctctggcgct
     9361 acatgcaatt gttgaggctg gtgcggcctg gttaccgctg gataccggct atccggacga
     9421 tcgcctgaaa atgatgctgg aagatgcgcg tccgtcgctg ttaatcacca ccgacgatca
     9481 actgccgcgc tttgccgatg ttccagattt aaccagcctt tgctataacg ccccgcttac
     9541 accgcagggc agtgcgccgc tgaaactttc acaaccgcac cacacggctt atatcatctt
     9601 tacctctggc tccaccggca ggccgaaagg ggtaatggtc gggcagacgg ctatcgttaa
     9661 ccgtctgctt tggatgcaaa accactaccc gcttacaggc gaagatgtcg ttgcccaaaa
     9721 aacgccgtgc agttttgatg tctcggtgtg ggagtttttc tggccgttta ttgccggggc
     9781 taaactggtg atggctgaac cggaagcgca ccgcgacccg ctcgctatgc agcaattctt
     9841 tgccgaatat ggtgtaacga ccacgcactt tgtgccgtcg atgctggcgg catttgttgc
     9901 atcgctgact ccgcaaaccg ctcgccagaa ttgcgcgacg ttgaaacagg ttttctgtag
     9961 tggtgaggcc ttacccgctg atttatgccg cgaatggcaa cagttaacca gcgtgccgtt
    10021 gcataatcta tatggcccga cggaagcggc ggtagatgtc agctggtatc cggcttttgg
    10081 cgaggaactg gcacaggtgc gcggcagcag tgtgccgatt ggttatccgg tgtggaatac
    10141 gggcctgcgt attcttgatg cgatgatgca tccggtgccg ccgggtgtgg cgggagatct
    10201 ctatctcacc ggtattcaac tggcgcaggg gtatcttgga cgacccgatc tgaccgccag
    10261 ccgctttatt gccgatcctt ttgcccctgg cgaacggatg taccgtaccg gagacgttgc
    10321 ccgctggctg gataacggcg cggtggagta cctcgggcgc agtgacgatc agctaaaaat
    10381 tcgcgggcag cgtatcgaac tgggcgaaat cgatcgcgtg atgcaggcgc tgccggatgt
    10441 cgaacaagcc gttacccacg cctgtgtgat taaccaggcg gcagccactg gtggtgatgc
    10501 gcgtcagttg gtgggctatc tggtgtcgca atcgggcctg ccgttggata ccagcgcatt
    10561 acaggctcag cttcgcgaaa cattgccacc acatatggtg cccgtggttc tgctgcaact
    10621 tccacagtta cctcttagcg ccaacggcaa gctggatcgc aaagccttac cgttgccgga
    10681 actgaaagca caagcgccgg ggcgtgcgcc gaaagcgggc agtgaaacga ttatcgccgc
    10741 ggcattctcg tcgttgctgg gttgtgacgt gcaggatgcc gatgctgatt tctttgcgct
    10801 tggcggtcat tcgctactgg caatgaaact ggcagcgcag ttaagtcgac agtttgttcg
    10861 tcaggtgacg ccggggcagg tgatggtcgc gtcaactgtc gccaaactgg caacgattat
    10921 tgatggtgaa gaagacagca cccagcgcat gggattcgaa accattctgc cgttgcgtga
    10981 aggtaatggc ccgacgctgt tttgtttcca tccggcctcc ggttttgcct ggcagtttag
    11041 cgtgctctcg cgttatctcg attcacaatg gtcgattatc ggcattcagt cgccgcgccc
    11101 tcatggcccc atgcagacgg cggcaaacct ggatgaagtc tgcgaagcgc atctggcaac
    11161 gttacttgaa caacaaccgc acggtcctta ttacctgctg ggttattcac ttggcggtac
    11221 gctggcgcag ggcattgcgg cgcgactgcg tgcccgtggc gaacaggtgg catttcttgg
    11281 cttgctggat acctggccgc cagaaacgca aaactggcag gaaaaagaag ccaatggtct
    11341 ggacccggaa gtgctggcgg agattaaccg cgagcgcgag gccttcctgg cggcacagca
    11401 gggaagtact tcaacggagt tgtttaccac cattgaaggc aactacgctg atgctgtgcg
    11461 cctgctgacg actgctcata gtgtaccgtt tgacggtaaa gcgacgctgt ttgttgctga
    11521 acgcgcgctt caggaaggta tgaatcccga acgcgcctgg tcgccgtgga tagccgagct
    11581 ggatatctat cgtcaggatt gtgcgcatgt ggatattatc tctccagggg cgtttgaaaa
    11641 aattgggccg attattcgcg ctacgc
//
LOCUS       DAZGHF010000142        11647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGHF010000142 DAZGHF010000000
VERSION     DAZGHF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.143"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            183..497
                     /gene="pspE"
                     /locus_tag="ACHK3A_004107"
     CDS             183..497
                     /gene="pspE"
                     /locus_tag="ACHK3A_004107"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO8742120.1"
                     /translation="MFKKGLLALTLMFSLPVFAAEYWIDVRIPKEYQKEHVQGAINIP
                     LKEMKERITTAVPDKNDTIKVYCNVGRQSEQAKEILNEMGYIHVENAGGMKDIEMPKV
                     KG"
     gene            1452..1739
                     /locus_tag="ACHK3A_004108"
     CDS             1452..1739
                     /locus_tag="ACHK3A_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO8742121.1"
                     /translation="MCKIKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQ
                     ALSAWLAQEEERNRLTLEALDDVTYGQVIDHQAVQARADSLSTDNPLPVPR"
     gene            1739..1864
                     /locus_tag="ACHK3A_004109"
                     /pseudo
     CDS             1739..1864
                     /locus_tag="ACHK3A_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2358..2783)
                     /gene="nudI"
                     /locus_tag="ACHK3A_004110"
     CDS             complement(2358..2783)
                     /gene="nudI"
                     /locus_tag="ACHK3A_004110"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO8742122.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQDIVWASPDTLKYMDLNEATRITFSQKGIL"
     gene            complement(2785..3492)
                     /locus_tag="ACHK3A_004111"
     CDS             complement(2785..3492)
                     /locus_tag="ACHK3A_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFO8742123.1"
                     /translation="MRYNYLYMDPWSADKKEKNKTRNPLYICLSGNSGVGKSTLLRRM
                     SKELFKVDPDTVAIDEKSVHHTLLPYLFDDTSNYGYLIQLNFMIQRALLIKSWLDKGF
                     NLIMERSHIEDYIFINFMLKSEYINKAQYEAYMVLWSRINELLTEPDIIAFINYPVAH
                     SLMHLNDDEIHGVRPEEFPDEEMKIKWITGWHAEYQHFTDNLPLHLRERIMVCAHPDD
                     IDDFSDKVIQKAMMLRT"
     gene            complement(3489..4355)
                     /locus_tag="ACHK3A_004112"
     CDS             complement(3489..4355)
                     /locus_tag="ACHK3A_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO8742124.1"
                     /translation="MDISTLLLDFRITSHCNLGCDLCFRTPGIQDSSLASARNVIERM
                     YKMGFRRIGFTGGEPTSRSDFIDLIEYAKQLGFMTYLSTVGHRFIMDHARLNPVLDWV
                     GIPIDGIDSLVNSEIRSDKMSNQHRVIKNILTWLPQNNNRINIKLTTVVSQSNINHLS
                     DIVCWVDSLPYKIQAWRFYQFCPLGVGKEKRDKLEINTDIFKEKMVMLKKQHPNMPIS
                     WATFEERDKANVVMEPNFDIIIPDGENYTLLGNMLTDSQDHIISAIFGNNEIMQKCKN
                     NRFWLDKMEFTV"
     gene            5077..6057
                     /locus_tag="ACHK3A_004113"
     CDS             5077..6057
                     /locus_tag="ACHK3A_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO8742125.1"
                     /translation="MRIFVDDGSTNIKLAWVEDGAVKTLISPNSFKPEWSMTLQGLTD
                     ANIPASANYEIDGEKYSFDQLSPDAVRTTETRYQYSDVNVVAIHHALMQSGIAPQPVD
                     IVVTLPLGEYLDENDQPNLANIERKKNNVRRAVTVQGRENFTIRKVSVLPESVPAGFD
                     VLKDLNDLESLLIVDIGGTTLDIAHVRSKMSGFTKTYCDPKTGVSIITEAVKHAMDVN
                     VSTRTSSYFADRLIQGRDDNEFLTRYIPNAEQREQILTVLRDREKKLQHRVADSVGRF
                     AGFTHVMVVGGGASLVAGAVKKATGVGDNRFFVSDNPQFDLVLGMMAMKG"
     gene            6060..6482
                     /locus_tag="ACHK3A_004114"
     CDS             6060..6482
                     /locus_tag="ACHK3A_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO8742126.1"
                     /translation="MADNRKCSFYIYPERNAADRVADRFLEKLPQKERGRAMRAMMLC
                     GAALMKQDERLPFLIAEFLTDSTSMQDIQRIISSTLPQQENGEVVRLLEAFLQSADNN
                     AKAILPAVDSATQEISAPVDQNLLETRNNIKNLFPDDE"
     gene            6506..6790
                     /locus_tag="ACHK3A_004115"
     CDS             6506..6790
                     /locus_tag="ACHK3A_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742127.1"
                     /translation="MLRPFFFSDKGCRRAPLFTAPADAARQERCLWSVSRVRPARDGR
                     FYRSRLASVYASQSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRTR"
     gene            6898..7800
                     /locus_tag="ACHK3A_004116"
     CDS             6898..7800
                     /locus_tag="ACHK3A_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO8742128.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRCATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGSEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNICSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSHP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            7797..8108
                     /locus_tag="ACHK3A_004117"
     CDS             7797..8108
                     /locus_tag="ACHK3A_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742129.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASQSPFSDERPSSRFRGITLPSERRRLRYSTVGLTRYRT
                     R"
     gene            8195..8878
                     /locus_tag="ACHK3A_004118"
     CDS             8195..8878
                     /locus_tag="ACHK3A_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO8742130.1"
                     /translation="MSRFILGDCVRVMATFPDNAVDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            8875..9096
                     /locus_tag="ACHK3A_004119"
     CDS             8875..9096
                     /locus_tag="ACHK3A_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742131.1"
                     /translation="MTNYSGFEDMREQVAELANTMCDLRTTMNEMEQRYSDADTLPER
                     LVRQTLFRASYLFMKAYNEILELDYCFKD"
     gene            9056..9545
                     /locus_tag="ACHK3A_004120"
                     /pseudo
     CDS             9056..9545
                     /locus_tag="ACHK3A_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            9590..10360
                     /locus_tag="ACHK3A_004121"
     CDS             9590..10360
                     /locus_tag="ACHK3A_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742132.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDVSRLATLLISRHDGVLPPVLASAMQKLTGTVAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLNAILNRGARFSSVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            10775..11200
                     /locus_tag="ACHK3A_004122"
     CDS             10775..11200
                     /locus_tag="ACHK3A_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8742133.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            11249..11440
                     /locus_tag="ACHK3A_004123"
     CDS             11249..11440
                     /locus_tag="ACHK3A_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742134.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            11403..>11647
                     /locus_tag="ACHK3A_004124"
     CDS             11403..>11647
                     /locus_tag="ACHK3A_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742135.1"
                     /translation="MVTTSFRAAETGDTGQAVEAACQPARKPLADWRLPEGMRATLAR
                     RPLPFPFVMCPECFFLMLCFCGYCSSSSSSFRSSPVYG"
BASE COUNT         2825 a         2779 c         2984 g         3059 t
ORIGIN      
        1 acaggtcggt tttaattctg acgggattca aaggtgcttc cctttggccg gtcgacgtat
       61 caaaggggca cccttttgaa aacccgaata cgccttaagt agaaaatgtg agaagtgatt
      121 cgcaaaaatt attaaacatc aagcatatta tagatgataa tcatcaatta aaggagagtt
      181 acatgtttaa aaaaggctta cttgctctga cactgatgtt ttcacttccc gtattcgctg
      241 ctgaatattg gatcgatgtc cgtattccaa aagagtatca gaaagagcac gttcaggggg
      301 ctataaatat tcctctgaag gaaatgaaag agcgtattac taccgcagtt cctgataaaa
      361 atgatactat taaagtttat tgcaacgttg gacgccagtc tgagcaggca aaagaaattc
      421 tcaatgagat gggatatatc cacgttgaga atgccggagg gatgaaagac atcgaaatgc
      481 caaaggttaa aggttaaagg ttaaaggtta aaaaatttca ctatcagtac acgccaaaag
      541 tgcttaacaa ttagattaat tctttatacc ggaaatttcg gcggttttac cgccgaatcc
      601 tttttcgctg gaacgttaga aaggttgagg ttttgttgaa taagtcagat ttcgggtaag
      661 tctctctgta tctggttggg ttttcaggca attcgcacgc tttcaggcat acccaccgtc
      721 gtcattttgt tcaggttgct gccgttgcaa tgaagcttgt ggctaaccgt aatcctgttc
      781 cggcagaata tttttaagat aatgaacaca gacggaaact ggtgataaga tagccgacgg
      841 atatataact caatgaaaac acatccccat tgacggacca cgccatactc gcacttcgca
      901 ttcatgtagg ttgcaggaga tccatttctt aattatttac ggtggtgcgt aagtgctcca
      961 ttttcgtaca ggttctcttc cgcttcctcg tcgatccgct cactacgttc ggtcatcgac
     1021 ttgcggcgag cgggggttcc tctcgtatat cgttgacgat caagatgtta tctatttcgg
     1081 tcgagccgtt ggagtcccgt taaatgacct caatcgttct gtaacgcgct caatggcgtc
     1141 agggcatggg aggcgagaaa attcgttaca gctctcagga ggtcaggcac gccgatactg
     1201 ttgcggagca gaatctgccc agaggcgtgg cccgtatacc tgagcttccc cgtttcactc
     1261 cagcacccgt tctatgtccc gcgtcagcac cggagaacta ctgacatccc ggcttacgct
     1321 aggctgcggc ggttttactc cggtgtcaca cataaccact gcgtgctgct ttacatgcgc
     1381 tgacgcgcat tatttgcctt gttctgtctg aatataatca atatgattaa atgtgattaa
     1441 caactaatcg gatatgcaaa attaaaaaca atactgcaca ggcaacaaaa gtaattaccg
     1501 cgcatgtgcc attacctatg gctgataaag tcgaccagat ggccgccaga ctggaacgct
     1561 cccggggctg gattatcaaa caggctcttt ctgcatggct tgcccaggag gaggagcgta
     1621 atcgcctgac tctggaagcc ttggacgatg tgacatacgg acaggttatc gaccatcagg
     1681 ctgtacaggc ccgggcagac agcctcagta ctgacaatcc gttaccggtg cctcgctgat
     1741 ggaactgaag tggaccagta aggcgctttc tgatttggca cggttatatg attttctggc
     1801 gctggctagt aaaccagttg cagccagaac agtgcagtcc ctgacacagg cgtcggtcat
     1861 tctggggtca gtttggatat cggaaaattt tgtacgttaa gcgcattatt taatcaatcg
     1921 agaagcgtaa cggattgatt ttgagtcagc ggctatcgct tttaagtcgt cgctctttcg
     1981 gtatcgcatt taggtgatcc gattaaagtc aaatcctctg aaaacgccgt atagcacggg
     2041 agtacaccct accgcaatag cgcaaccgta caatattgta cgtttcccaa acgcagccca
     2101 cgtacgttag tgaagtaact gtctgatata tcaaaacata atggacatag aaaaacgcca
     2161 tcaaaactgt gtctttttaa tcgaaaattg gcacttaacg gacggcattg tctactaatc
     2221 gatttttaag acgataattt tcctcttacc gtacgttttc gtacggttgg ccctcaaacc
     2281 ccatccttga gtagtggcat aagaaaacgg ctccctgttg tggaagccgt tatcagttgc
     2341 acgattagca ttggagttta aaggatacct ttctgagaaa atgtgatccg ggttgcttca
     2401 tttaaatcca tatattttag ggtgtcagga gatgcccaga ctatatcctg aaactcctca
     2461 ttaaatgtaa ctgtacggtt tgtactgata cagtcgaata tgaggtaaat catgtaaatg
     2521 tcctcgtggg taccatccgg gtacgttttt gtacggatat catcacgaaa ggcccagggt
     2581 tttacgtcag tgatatgcag ttcttttccc agttcttcct ttatttcacg cctcaatgct
     2641 gtttccatag tctctccggg ttccattcct ccgccgggca gtgcccattg gcccgggaat
     2701 actccacgat cagaggccat tttacaaagt agaaactcac cttcattttg tatcacaggg
     2761 cacactatta ctctctggcg cattttatgt cctcaacatc atggcttttt ggataacctt
     2821 atcagaaaaa tcatcaatgt catccgggtg agcacatacc ataatccgct cacgaaggtg
     2881 taacggaaga ttatcagtaa aatgttgata ttccgcatgc catcctgtga tccattttat
     2941 cttcatttcc tcatctggaa attcttcagg ccgtacacca tgaatttcat catcattgag
     3001 atgcatgagt gaatgtgcga cgggataatt aatgaatgct ataatgtctg gctctgtaag
     3061 taattcattt attctactcc agagcaccat gtatgcttca tattgtgctt tatttatata
     3121 ttcggacttc agcatgaagt tgataaaaat gtaatcctca atatgggatc tctccataat
     3181 aaggttaaac cctttatcca gccaagattt tatcagtaat gctcgttgta tcataaaatt
     3241 aagctggatt agataaccgt agttactggt atcgtcaaaa aggtaaggaa ggagagtgtg
     3301 gtggacactt ttttcatcta tcgcgacagt gtctggatct accttaaata actcctttga
     3361 catgcgtctc agaagagtac ttttacctac accggaatta cccgacagac atatatacag
     3421 aggatttcta gttttattct tctctttttt atctgctgac caaggatcca tatacaagta
     3481 attatatctc atactgtaaa ctccattttg tccaaccaaa agcggttatt cttacatttt
     3541 tgcatgattt cattatttcc gaaaattgca ctgattatat gatcttgtga atctgtcagc
     3601 atatttccca acagagtata gttttctcca tcagggataa taatgtcaaa attgggttcc
     3661 ataacaacat tggctttgtc ccgttcttcg aaagttgccc aactaatggg catgttaggg
     3721 tgttgttttt ttagcattac cattttctct ttaaagatat ccgtattgat ttccagttta
     3781 tctctttttt cttttccgac tcctagagga cagaactggt aaaatcgcca agcttggatc
     3841 ttatagggca gagaatcaac ccagcaaaca atatcactga gatgattaat gttagactga
     3901 gatacgacgg tcgtgagttt tatattaatt cgattattat tttggggtaa ccatgttaat
     3961 atatttttga taaccctgtg ttggttactc attttatcac tgcgaatttc tgaattgaca
     4021 agactgtcaa ttccatcaat tggtattcct acccagtcta acaccggatt taaccgagca
     4081 tgatccataa taaatcgatg cccgaccgtg gagagatatg tcataaaacc cagttgctta
     4141 gcgtattcaa tcagatcaat gaaatcactc cgggatgtag gttcccctcc ggtaaaaccg
     4201 atacgacgga atcccatttt atacatccgc tcaattacat tacgagcact agcaagagaa
     4261 gagtcctgaa ttccaggtgt acgaaaacac agatcacatc ctaggttaca atgtgaagtt
     4321 attctgaaat ctaaaagaag ggtgctaata tccataacaa gtcctctgac tatgatacat
     4381 caataaccat taaaacataa tggttattgc agaccatgac aaagcgcaac cttcggctta
     4441 ccagggatat aatatctgcc agtaatcaga tcagtgggtt gcgcaaggaa ctcatgaatg
     4501 atgagttccg gtaatcgcat tactggaagc ccatgattgg gccgcttgga attcggaaaa
     4561 taaaccacgc taagagaatt gagttgaacg agaggtgaca tccaagccag ctaactcacc
     4621 gaacgtaaag gctgtacgaa atgttccgac cgtccgataa ctgtttggct tacttccgta
     4681 cattcccctg cgtagatgta cataattgta cgatttcgga caccaaaacc gctgttgtcc
     4741 cgccattctg gcgattcctg aaagttattt gactaaaaaa tagtcttaac gtggtttatt
     4801 ttccgaattc caaactgacc ccagatacag catgtgctgg atgagaaccc cttctccggt
     4861 cacctgttca gttttttctt gattttaact gtatttatct tttgttgttc ttgtttgttc
     4921 cctggtatgc tgtttgagtc ggtttttgtc tttatttttc tttatttttc tgtttgtggc
     4981 cgatatcagg tctttactga tgcgtgtttt taggtgataa tcgttttcga caccatattt
     5041 ttctaaaaaa atccatttta ttctgtgagg ttctggatgc gtatctttgt agatgacggt
     5101 tctaccaaca tcaaactggc ctgggttgaa gacggtgcag tgaaaacgtt aattagtccg
     5161 aatagtttta agccggaatg gtctatgacg ctgcagggac tgactgatgc caatataccc
     5221 gcttccgcaa actatgagat tgatggagag aaatacagct ttgatcaatt gagtccggat
     5281 gcagtgcgca ccacagaaac gcgttatcag tacagcgacg ttaatgtggt tgctattcat
     5341 catgcgttga tgcagtcagg tattgctccg caacctgttg atattgttgt gactttacct
     5401 ttaggtgagt atctggatga gaacgatcag ccaaatctgg ctaatatcga acgtaagaaa
     5461 aataatgtca gacgagctgt aacagttcag gggcgtgaaa actttaccat tcgtaaagtc
     5521 agtgtcttgc ctgagtccgt gcctgccggt tttgacgtgc tgaaagatct gaatgatctg
     5581 gagtcgctgt tgatcgttga tatcggtgga acgacccttg atattgcaca tgtccgcagc
     5641 aaaatgtcag gtttcacaaa aacttattgt gaccccaaga caggcgtttc catcattact
     5701 gaagcagtta agcatgcgat ggatgtgaat gtgagtacac gaaccagttc ttattttgca
     5761 gatcgtctaa tccagggccg ggatgacaat gaattcctga ctcgttatat cccgaatgca
     5821 gaacagcgtg agcagatcct gactgtatta cgtgaccggg aaaaaaaact gcagcatcgt
     5881 gtggcggatt cagtaggacg ctttgctggt tttactcatg ttatggttgt tggcggtgga
     5941 gccagccttg tggctggggc agtaaaaaaa gcgacaggag tgggtgataa ccgtttcttt
     6001 gtcagtgaca atcctcagtt cgatctggtg ttgggaatga tggcaatgaa gggatgagta
     6061 tggctgataa tcgtaagtgc tctttttata tttatccgga acgaaatgca gctgatcggg
     6121 tggctgacag gtttcttgaa aaattgcctc agaaagaacg tggtcgggca atgcgggcca
     6181 tgatgttgtg tggtgctgca ttgatgaaac aggatgaacg attgccgttc ctgatagctg
     6241 aattcctgac agacagcacg tctatgcagg atattcaacg aattatcagt agtacgctac
     6301 cacaacagga aaatggtgaa gttgttcgtt tgctggaggc atttctgcag tctgccgata
     6361 ataatgcaaa agcgatcctg cctgcggttg attctgctac gcaagaaata tctgctccag
     6421 tcgatcagaa tctcctggaa acaagaaaca acattaagaa cttgtttccg gatgatgaat
     6481 aagaactgaa ctctgttaaa ggacgctgtt gcgtcctttt ttcttttccg acaaaggatg
     6541 tcgccgggct cctcttttta ctgcgccggc tgacgcggcg cggcaggaac gctgcctgtg
     6601 gtcagtgtcc cgcgtccggc cggcacggga cgggcgcttt taccgctccc ggctggcgtc
     6661 ggtgtacgcc agccagtcgc ccttttctga tgagcgccct tcaagccgct ttcgcggcat
     6721 aaccttgccg tcaaaaagac gacggctgcg gtattccacg gtcggcctga cccgttaccg
     6781 gacgcggtga acagcccaca ggcagcgggg aactggtgca cagggcgccc tcccggtgct
     6841 ctctgctgaa gcagaagtga gaggagaatt cccatgccaa actggtgcag taaccgtatg
     6901 catttttctg gtgaaccggc acagatcgct gagattaaac gactggccag tggtgccgtc
     6961 acaccgtttt atcgctgcgc cacaaatgaa ggtattcagc tgtttctggc cggaagtgcc
     7021 ggacttctgc agatcactga aaatatccgc tctgagcagt gccccggagt gacggctgcc
     7081 ggacgtggtg ctgtatcgac ggaaaatatc gcgttcaccc gctggctgac acacctgcag
     7141 aacggtgtac tgctggatga gcaaaactgc ctgatgctgc atgaactctg gctgcagagc
     7201 ggtactggac agcgtcgctg ggaaggatta ccggatgatg tcagggaaac catcactgtg
     7261 catttcaccg caaaaagagg tgactggtgt gacatctggg gtagcgagga tgtatcggtg
     7321 tggtggaacc gtctgtgtga caacgtagtg ccggaaaaaa ccatgccgtt tgacctgctg
     7381 acggttctgc cgacccgcct ggatgttgaa gtgaatggct ttaacggtgg tgttctgaac
     7441 ggcgttcctt ctgcatatca ctggtataca gaacggtatg gcgtgaagtg gccttgtggg
     7501 tatgacctga atatctgtag tcagggagac aacttcattc aggttgattt cgacacgccg
     7561 tggtgtcagc cggaaagcga cgttattgca gaattaagcc gccgtttcag ctgcacactg
     7621 gagcactggt atgccgaaca gggctgcaat ttctgtggct ggcagcgata tgagcgtgga
     7681 gagcttgttg atgtgctgtg gggggagctg gaatggtcac atccgacaga tgatgacgag
     7741 ttgccggaag tcaccggacc tgcgtggata gtcgacaagg tggcgcatta tggcggatga
     7801 agtatgacgg agacgggcgg gcaaccgccc gtttcttttc cggtaaagga tgtcgccgtg
     7861 ctcctctttt tactgcgccg gctgacgcgg cgcggcataa acgctgcctg tggtcagtgt
     7921 cccgcgtccg gcgggcacgg gacgggcgct tttaccgctc ccggctggcg tcggtgacag
     7981 tgtacgccag ccagtcgccc ttttctgatg aacgcccttc aagccgcttt cgcggcataa
     8041 ccttgccgtc agaaagacga cggctgcggt attccacggt cggcctgacc cgttaccgga
     8101 cgcgctgaac agcccacagg cagcggggaa cgggcaccac aggggtgccc tcccggtgcc
     8161 ctcccggtgc cctctgtaag agaaggaggc ttttatgtcc cgttttatcc tgggtgactg
     8221 cgtgcgagtt atggccacgt ttccggacaa tgctgtcgat ttcatcctca ccgatccgcc
     8281 atatctcgtc ggtttccgtg accgttccgg gcgcaccatt gctggtgacg ttaatgatga
     8341 ctggctgcaa cctgccagca atgagatgta ccgcgtactc aaaaaagacg cgctgatggt
     8401 cagcttctac ggctggcacc gtattgaccg ctttatggcg gcctggaaac gcgccggatt
     8461 cagcgtggca gggcacctcg tgttcaccaa aaactacaca tccaaatctg cgtatgtcgc
     8521 gtaccgccac gaatgcgctt acatcctggc aaagggcagt ccggcccggc cccgtaaccc
     8581 gctgccggat gtgctgggct ggaaatattc gggcaaccgc catcacccga cggaaaagcc
     8641 cgttaccagc ctgcaaccgc tgattgagag cttcacacat ccgaacgcaa ttgtgctgga
     8701 tccgtttgca ggcagcggtt caacctgcgt ggccgccctt cagtccggac gccggtatat
     8761 cggtatcgag ctgcttgaac agtatcaccg tgccggacag caacgactgg ccgccgtgca
     8821 acgggccatg cagcaggggg ccgcgaatga taactggttt gaaccggagg ctgcatgacg
     8881 aattacagcg gattcgaaga tatgcgtgaa caggtggcgg aactggccaa taccatgtgc
     8941 gacctgcgta caaccatgaa tgagatggag cagcggtaca gcgatgcgga caccctcccg
     9001 gaacgtctgg tgcgccagac actctttcgt gccagttacc tgtttatgaa ggcatataac
     9061 gaaattctgg aactggatta ctgcttcaaa gattgagggg agagaagagt atgtacggaa
     9121 catgcgaaac gctatgccgg gcgctggcag caaagtatcc gggagacacg ccgttgatgc
     9181 tggttatctg gtccccggaa gagattcagg ccctcgctga cggaatggat atttccctgt
     9241 ccgatcatga aatcaggacc gtcctggcgc gcctggagga catcccggaa gaccagcgga
     9301 ctgagtccgg tatttcttcc ggcgtggcga tggagatcat caataacgtg agagaaaacc
     9361 gccaggtgac cgtcccggct gaactgctgg cgtccctgat tcagaccgcc gaacaggcac
     9421 tgtggaaacg tgaatgggct gcccgtgatt acggcctcgc cgtcccggaa tgcgttaccc
     9481 gccgtcaggc ggtggttaat caggcccgca tcctgctgaa aaacaacaca cacgaaaacg
     9541 actgatgtta tcgccgcctt cgggcggcga ttcagggaga ccatcaatca tgaacaaaac
     9601 gctgaatgca ctggtttgtc gccacgcccg taacctgctg ctggcgcagg gctggccgga
     9661 agagacggat attgaacaac tgaatccgca ctatccgggc tggatcagca tttatgtgcg
     9721 actggatgta tcccggctgg cgacgttact tatcagccgc catgatggcg tgctgccgcc
     9781 ggtcctggcg tctgccatgc aaaaactgac cggaaccgtg gcggaacttg tactgtccgg
     9841 cagccagtgg caggcgctgc cggtacttcc ggcagacgga acgcaggtgt ctttcccgta
     9901 tgccggtgaa tggctgacgg aggaagaaat cagggcagtt cttgatgcgg tgcgcaatgc
     9961 agtacgaagc atctgttacc aggtggcaga cgatgcgcgg cgtatccggg cggcgctgac
    10021 caccaccggt cagacgttgc tgacccgcca gacgcgccgc tttcgtctgg ttgtgaagga
    10081 aagcgattac ccctgctggc ttgatgaaga tgacgaaaat ctgcccgtgg tgctcaatgc
    10141 cattctgaac cgtggcgccc gtttttcctc tgtggaaatg tacctggtct gtgagtgtgt
    10201 ggagcatatc ctggccagtg gtctggtctg tgatgtgctg cgtataccgg atgaaccgtc
    10261 ccgtcgctgg tttgaccggg atattctgcg ggaggttgtt ctggaggcac gggatgaaat
    10321 ccgcagcatg gcggatgcac tggcgaaaat ccggaaatga tgacgctggc ggggaaaaat
    10381 ctccaccttt tcctgccggt ccccggacag agcaaaagga tgtcgccgca ggctccgctt
    10441 tacccggcca tgcggggcat ggccttgtgg gttttcagtt atgtggcctc agcgttgtat
    10501 gcgggcggtg ccgtgcctcc atcttagccg ggctggcggg gatgcaaggg taaaatgcac
    10561 gcggcggtca cccggtccct ccttcccgtc tgccgtgatt ttccggtcgt tcacccgctc
    10621 agatttcgcg gtctcaccct ccaactcccg tcagccgtgt gcgtcaggct gcggcttccc
    10681 ttgcgccctg tgcatccccg ccttatcgcc cggcttttat ggaggcacgg caccgcccgg
    10741 tgtcgcggaa tatgtgaaca acggaggaaa tcccatgcag tatgcgaaac ctgtcactct
    10801 gaatgttgaa gagtgcgacc gcctttcttt tctgccttac ctgtttggca acgattttct
    10861 gtatgccgag gcgtatgtgt acgcgctggc gcaaaaaatg atgccggaat atcagggagg
    10921 cttctggcat ttcatccgcc tgccggacgg tggcggttac atgatgccgg atggcgaccg
    10981 ttttcatctg gtcaacggtg aaaactggtt tgaccgcact gtgagtgctg atgccgcagg
    11041 tatcatcctg acttcccttg tgattaaccg ccagttgtgg ctgtaccacg acagcgggga
    11101 tgcgggactg acacacctgt accggatgcg tgatgcgcag ttgtggagcc acatcgaatt
    11161 tcaccccgaa tgcaatgcga tttacgcagc actggactga ttaacggacg gggcggcgat
    11221 gccgcccctg taaaggaggc gaagaaaaat gaacatcagc acagaaaccc gcgaaattct
    11281 gcgtaattac agggccgtga ttaatgcgcg gcgtcgtgaa atggggcaga aaccgctcac
    11341 cacagcacag attgttgatg aaatctgcga ttttgtggcg aatcagcagg cggttttcct
    11401 cggtggtcac tacatccttc agggcagcag aaacaggtga tacagggcag gcggtggagg
    11461 ccgcctgtca gcccgcgcga aaacccctgg cagactggcg cctgccggaa gggatgcggg
    11521 ccacgctggc gcgtcgcccg ttacccttcc cgtttgtgat gtgtccggaa tgttttttcc
    11581 tgatgttatg tttctgtggt tactgttctt cttcttcttc ctctttccgt tcctctcctg
    11641 tttatgg
//
LOCUS       DAZGHF010000143        11624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGHF010000143 DAZGHF010000000
VERSION     DAZGHF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.144"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(16..609)
                     /gene="yhgN"
                     /locus_tag="ACHK3A_004125"
     CDS             complement(16..609)
                     /gene="yhgN"
                     /locus_tag="ACHK3A_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO8742136.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            801..1904
                     /gene="asd"
                     /locus_tag="ACHK3A_004126"
     CDS             801..1904
                     /gene="asd"
                     /locus_tag="ACHK3A_004126"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO8742137.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            2177..4363
                     /gene="glgB"
                     /locus_tag="ACHK3A_004127"
     CDS             2177..4363
                     /gene="glgB"
                     /locus_tag="ACHK3A_004127"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO8742138.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVRTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNVGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            4360..6333
                     /gene="glgX"
                     /locus_tag="ACHK3A_004128"
     CDS             4360..6333
                     /gene="glgX"
                     /locus_tag="ACHK3A_004128"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO8742139.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            6351..7646
                     /gene="glgC"
                     /locus_tag="ACHK3A_004129"
     CDS             6351..7646
                     /gene="glgC"
                     /locus_tag="ACHK3A_004129"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO8742140.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            7646..9079
                     /gene="glgA"
                     /locus_tag="ACHK3A_004130"
     CDS             7646..9079
                     /gene="glgA"
                     /locus_tag="ACHK3A_004130"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO8742141.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            9098..11545
                     /gene="glgP"
                     /locus_tag="ACHK3A_004131"
     CDS             9098..11545
                     /gene="glgP"
                     /locus_tag="ACHK3A_004131"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO8742142.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         2714 a         2929 c         3180 g         2801 t
ORIGIN      
        1 gaaaaactct cctcgttacc ccttcatcca cattcgaatg ccgtcgagga acatctgggt
       61 tgccatcatc accagaatca atcccatcag gcgttcaagt gcgttcaccc ctttctcgcc
      121 cagcagacgt aaaaatagcg aagactgtag caggatgaca aaggtgccgc cccaggccag
      181 cagcagagca atcaccagat gtcccatctg attcgggtac tgatgagaca acaacatcag
      241 cgtggcgaga atagtcggcc cggcgactaa cggaattgcc aacggcacga taaacggctc
      301 ttcacctgcc ggaagcccgc tgctatttcc tgaagcgctg gggaaaatca tcttaatggc
      361 gatcagaaac agaatgatgc cgccagaaat ggagacggtt tctgctcgca ggctgagaaa
      421 tgccagaatt ttctcacccg caaacaggaa caccagcatc accaggagag caataagcaa
      481 ctctcgcacc atgattgccc gccgtctttt cggttcagta tgtttcagta cggacatgaa
      541 aataggtagg tttccgagcg gatccataat caggatcaat aaaactgctg cagaaatgat
      601 ttcattcata actcaaattc cctgataatt gccgcggact ttctgcgtgc taacaaagca
      661 ggataagtcg cattactgat ggcttcgcta tcattgatta atttcacttg cgactttggc
      721 tgctttttgt atggtgaaag atgtgccaag aggagaccgg cacatttata cagcacacat
      781 ctttgcagga aaaaacgctt atgaaaaatg ttggttttat cggctggcgc ggtatggtcg
      841 gctccgttct catgcaacgc atggttgaag agcgcgactt cgacgccatt cgccctgtct
      901 tcttttctac ttctcagctt ggtcaggctg cgccgtcttt tggcggaacc actggcacac
      961 ttcaggatgc ctttgatctg gaggcgctaa aggccctcga tatcattgtg acctgtcagg
     1021 gcggcgatta taccaacgaa atctatccaa agcttcgtga aagcggatgg caaggttact
     1081 ggattgacgc agcatcgtct ctgcgcatga aagatgatgc catcatcatt cttgaccccg
     1141 tcaatcagga cgtcattacc gatggattaa ataatggcat caggactttt gttggcggta
     1201 actgtaccgt aagcctgatg ttgatgtcgt tgggtggttt attcgccaat gatcttgttg
     1261 attgggtgtc cgttgcaacc taccaggccg cttccggcgg tggtgcgcga catatgcgtg
     1321 agttattaac ccagatgggc catctgtatg gccatgtggc agatgaactc gcgaccccgt
     1381 cctctgctat tctcgatatc gaacgcaaag tcacaacctt aacccgtagc ggtgagctgc
     1441 cggtggataa ctttggcgtg ccgctggcgg gtagcctgat tccgtggatc gacaaacagc
     1501 tcgataacgg tcagagccgc gaagagtgga aagggcaggc ggaaaccaac aagatcctca
     1561 acacatcttc cgtaattccg gtagatggtt tatgtgtgcg tgtcggggca ttgcgctgcc
     1621 acagccaggc attcactatt aaattgaaaa aagatgtgtc tattccgacc gtggaagaac
     1681 tgctggctgc acacaatccg tgggcgaaag tcgttccgaa cgatcgggaa atcactatgc
     1741 gtgagctaac cccagctgcc gttaccggca cgctgaccac gccggtaggc cgcctgcgta
     1801 agctgaatat gggaccggag ttcctgtcag cctttaccgt gggtgaccag ctgctgtggg
     1861 gggccgcgga gccgttgcgt cggatgcttc gtcaactggc gtaatcttta ttcattaaat
     1921 ctggggcgcg atgccgcccc tgttagtgcg taatacagga gtaagcgcag atgtttcatg
     1981 atttaccggg agttaaatag agcattggct attctttaag ggtggctgaa tacatgagta
     2041 ttcacagcct tacctgaagt gaggacgacg cagagaggat gcacagagtg ctgcgccgtt
     2101 caggtcaaaa aaatgtcaca accagaagtc aaaaatccaa ttggatgggg tgacacaata
     2161 aaacaggaag acaagcatgt ccgatcgtat cgatagagac gtgattaacg cgctaattgc
     2221 aggccatttt gcggatcctt tttccgtact gggaatgcat aaaaccaccg cgggactgga
     2281 agtccgtgcc cttttacccg acgctaccga tgtgtgggtg attgaaccga aaaccgggcg
     2341 caaactcgca aaactggagt gtctcgactc acggggattc tttagcggcg tcattccgcg
     2401 acgtaagaat tttttccgct atcagttggc tgttgtctgg catggtcagc aaaacctgat
     2461 tgatgatcct taccgttttg gtccgctaat ccaggaaatg gatgcctggc tattatctga
     2521 aggtactcac ctgcgcccgt atgaaacctt aggcgcgcat gcagatacta tggatggcgt
     2581 cacaggtacg cgtttctctg tctgggctcc aaacgcccgt cgggtctcgg tggttgggca
     2641 attcaactac tgggacggtc gccgtcaccc gatgcgcctg cgtaaagaga gcggcatctg
     2701 ggaactgttt atccctgggg cgcataacgg tcagctctat aaatacgaga tgattgatgc
     2761 caatggcaac ttgcgtctga agtccgaccc ttatgccttc gaagcgcaaa tgcgcccgga
     2821 aaccgcgtct cttatttgcg ggctgccgga aaaggttgta cggactgaag agcgcaaaaa
     2881 agcgaatcag tttgatgcgc caatctctat ttatgaagtt catctgggct cctggcgtcg
     2941 ccacaccgac aacaatttct ggttgagcta ccgcgagctg gccgatcaac tggtgcctta
     3001 tgctaaatgg atgggcttta cccacctcga actactgccc attaacgagc atcccttcga
     3061 tggcagttgg ggttatcagc caaccggtct gtatgcaccg acccgccgtt ttggtactcg
     3121 cgacgacttc cgttatttca ttgatgccgc acacgcagct ggtctgaacg tgattctcga
     3181 ctgggtgcca ggccacttcc cgaccgatga ctttgcgctt gccgaatttg atggcacgaa
     3241 cttgtatgaa cacagcgatc cgcgcgaagg ctatcatcag gactggaaca cgctgatcta
     3301 caactatggt cgccgtgaag tcagtaactt ccttgtcggt aacgcgcttt actggattga
     3361 acgttttggt attgatgcgc tgcgcgtcga tgcggtagcg tcaatgattt atcgcgacta
     3421 cagccgtaaa gagggggagt ggatcccgaa cgaatttggc gggcgcgaga atcttgaagc
     3481 gattgaattc ttgcgtaata ccaaccgtat tcttggtgag caggtttccg gtgcggtgac
     3541 aatggctgag gagtctaccg atttccctgg cgtttctcgt ccgcaggata tgggcggtct
     3601 gggcttctgg tacaagtgga acctcggctg gatgcatgac accctggact acatgaagct
     3661 cgacccggtt tatcgtcagt atcatcacga taaactgacc ttcgggatgc tctacaacta
     3721 cactgaaaac ttcgtcctgc cgttgtcgca tgatgaagtg gtccacggta aaaaatcgat
     3781 tctcgaccgg atgccgggcg acgcatggca gaaattcgct aacctgcgcg cctactacgg
     3841 ctggatgtgg gcattcccgg gcaagaaact gctgttcatg gggaacgaat ttgcccaggg
     3901 ccgcgagtgg aaccatgacg ccagcctcga ctggcatctg ttggaaggcg gcgataactg
     3961 gcaccacggt gtccagcgtc tggtgcgcga tctgaacctc acctaccgcc accataaagc
     4021 gatgcatgaa ctggattttg acccgtacgg ttttgaatgg ctggtggtgg atgacaaaga
     4081 acgctcggtg ctgatctttg tgcgtcgcga taaagagggt aacgaaatca tcgttgccag
     4141 taactttact ccggtgccgc gtcatgatta tcgcttcggc attaaccagc cgggcaaatg
     4201 gcgtgaaatc ctcaataccg attccatgca ctatcacggc agtaatgtcg gcaatggcgg
     4261 cacggtacac agcgatgaga ttgccagcca cggtcgtcag cattcattaa gcctgacact
     4321 accaccgctg gccactatct ggctggttcg ggaggcagaa tgacacaact cgccattggc
     4381 aaacccgcac ccctcggcgc gcattacgac ggtcagggcg tcaacttcac acttttctcc
     4441 gctcatgccg agcgggtaga actgtgtgtc tttgacgcca atggccagga acatcgctat
     4501 gacttgccag ggcacagtgg cgacatttgg cacggttatc tgccggatgc gcgtccgggt
     4561 ttgcgttatg gttatcgcgt tcatggcccc tggcaacccg ccgaggggca tcgctttaac
     4621 ccggcgaagt tgttgattga tccttgcgcg cggcaaattg acggggaatt taaagataac
     4681 ccgctgctgc acgccggtca taatgaacct gactatcgcg acaacgccgc cattgcgccg
     4741 aaatgcgtag tggtggttga tcactatgac tgggaggatg atgctccgcc gcgcacgccg
     4801 tggggcagta ccatcattta tgaagcccat gtcaaaggat taacgtacct gcacccggag
     4861 atcccggtcg agatccgtgg cacttataaa gccctggggc atccggtgat gatcaactat
     4921 ttgaaacagt tgggcattac cgcgctggaa ctgctgccgg tggcgcagtt tgccagcgaa
     4981 ccacgcctgc aacgtatggg tctaagtaac tactggggtt ataacccggt ggcgatgttt
     5041 gcgctgcatc cggcgtatgc ctgctcccca gaaacggcgc tggatgagtt tcgcgatgca
     5101 atcaaagcac tgcataaagc gggtatcgaa gtcattcttg atatcgtgct caaccatagt
     5161 gcggaactgg acctcgacgg cccgttattc tctctgcgtg ggatcgacaa ccgtagctat
     5221 tattggataa gagaagacgg cgattatcac aactggaccg gttgtggcaa cacgctcaat
     5281 ttgagtcatc cggcggtggt ggattatgcc agcgcctgcc tgcgttattg ggtagaaacc
     5341 tgccacgtcg atggtttccg ctttgatctg gcggcagtca tgggccgtac gccagagttc
     5401 cgtcaggatg cgccgttgtt taccgctatc cagaactgcc cggtgctctc gcaggtgaag
     5461 ttaattgctg aaccgtggga tattgctcct ggtggttatc aggtgggaaa tttcccgccg
     5521 ctgtttgccg agtggaacga tcatttccgc gatgctgccc gtcgtttctg gcttcattat
     5581 gatttgcctc tgggggcgtt tgccgggcgt tttgctgcct ccagcgatgt ttttaaacgt
     5641 aatggtcgtc tgccgagtgc cgcgattaat ctcgtcaccg cgcatgacgg ttttacgctt
     5701 cgcgactgcg tttgcttcaa ccataaacac aatgaagcaa acggagaaga aaatcgcgac
     5761 gggaccaaca acaattacag taacaatcat ggtaaagaag ggttaggcgg tactcttgac
     5821 ctggttgaac ggcgtcgcga cagcattcac gccctgttaa caacgttgtt gctctcccag
     5881 ggtacgccga tgttactggc cggtgacgaa catggtcaca gccagcatgg caataacaat
     5941 gcctactgtc aggataacca attaacctgg ttggactggt cgcaggcaag cagtggttta
     6001 accgcattta ccgccgcgtt aatccatctg cgcaagcgca ttcccgcttt ggtggagaat
     6061 cgctggtggg aagaaggcga cggcaatgtc cgttggctaa atcgatatgc tcaaccttta
     6121 agcacggatg agtggcaaaa cgggccgaaa cagctgcaaa ttctgctctc ggatcgcttt
     6181 ttgatcgcaa ttaacgccac gcttgaggta acagagattg ttttacctgc tggggagtgg
     6241 cacgccattc ccccattcgc tggagaggat aacccagtga ttacggctgt ctggcaggga
     6301 cctgcacacg gattgtgtgt gttccagaga tgataaaaaa ggagttagtc atggttagtt
     6361 tagagaagaa cgatcactta atgttggcgc gccagctgcc attgaaatct gttgccctga
     6421 tactggcggg aggacgtggt acccgcctga aggatttaac caataagcga gcaaaaccgg
     6481 ccgtacactt cggcggtaag ttccgcatta tcgactttgc gctgtctaac tgcatcaact
     6541 ccgggatccg tcgtatgggc gtgatcaccc agtaccagtc ccacactctg gtgcagcaca
     6601 ttcagcgcgg ctggtcattc ttcaatgaag aaatgaacga gtttgtcgat ctgctgccag
     6661 cacagcagag aatgaaaggg gaaaactggt atcgcggcac cgcagatgca gttacccaaa
     6721 acctcgatat tatccgccgt tataaagcgg aatacgtggt gatcctggcg ggcgaccata
     6781 tctacaagca agactactcg cgtatgctta tcgaccacgt cgaaaaaggc gcacgttgca
     6841 ccgttgcttg tatgccagta ccgattgaag aagcctccgc atttggcgtt atggcggttg
     6901 atgagaacga taaaattatc gaattcgttg aaaaacctgc taacccaccg tcaatgccga
     6961 acgatccgag caaatctctg gcgagtatgg gtatctacgt ctttgacgcc gactatctgt
     7021 atgaactgct ggaagaagac gatcgcgatg agaactccag ccacgacttt ggcaaagatt
     7081 tgattcccaa gatcaccgaa gccggtctgg cctatgcgca cccgttcccg ctctcttgcg
     7141 tacaatccga cccggatgcc gagccgtact ggcgcgatgt gggtacgctg gaagcttact
     7201 ggaaagcgaa cctcgatctg gcctctgtgg tgccggaact ggatatgtac gatcgcaatt
     7261 ggccaattcg cacctacaat gaatcattac cgccagcgaa attcgtgcag gatcgctccg
     7321 gtagccatgg gatgaccctt aactcactgg tatccggcgg ttgtgtgatc tccggttcgg
     7381 tggtggtgca gtccgttctg ttctcgcgcg ttcgcgtgaa ttcattctgc aacattgatt
     7441 ccgccgtatt gttaccggaa gtatgggtag gtcgctcgtg ccgtctgcgc cgctgcgtca
     7501 tcgatcgtgc ttgtgttatt ccggaaggca tggtgattgg tgaaaacgca gaggaagatg
     7561 cacgtcgttt ctatcgttca gaagaaggca tcgtgctggt aacgcgcgaa atgctacgga
     7621 agttagggca taaacaggag cgataatgca ggttttacat gtatgttcag agatgttccc
     7681 gctgcttaaa accggcggtc tggctgatgt tattggggca ttacccgcag cacaaatcgc
     7741 agacggcgtt gacgctcgcg tactgttgcc tgcatttccc gatattcgcc gtggcgtgac
     7801 cgatgcgcag gtagtatccc gtcgtgatac cttcgccgga catatcacgc tgttgttcgg
     7861 tcattacaac ggggttggca tatacctgat tgacgcgccg catctctatg atcgtccggg
     7921 aagcccgtat cacgatacca acttatttgc ctataccgac aacgtattgc gttttgcgct
     7981 gctggggtgg gttggggcag aaatggccag cgggcttgac ccattctggc gtcctgatgt
     8041 ggtgcatgcg cacgactggc atgcaggcct tgcgcctgcg tatctggcgg cgcgcgggcg
     8101 tccggcgaag tcggtgttta ctgtgcacaa cctggcctat caaggcatgt tttatgcaca
     8161 tcacatgaat gacatccaat tgccatggtc attctttaat attcatgggc tggaattcaa
     8221 cggacaaatc tcttttctga aggccggtct gtactatgcc gatcacatta cggcagtcag
     8281 cccaacctac gctcgcgaga tcaccgaacc gcagtttgcc tacggtatgg aaggtctgtt
     8341 gcaacagcgt caccgtgaag ggcgtctttc cggcgtactg aacggcgtgg acgagaaaat
     8401 ctggagtcca gagacggact tactgttggc ctcgcgttac acccgcgata cgttggaaga
     8461 taaagcggaa aataagcgcc agttacaaat cgcaatgggg cttaaggttg acgataaagt
     8521 gccgcttttt gcagtggtga gccgtctgac tagccagaaa ggtctcgacc tggtgctgga
     8581 agccttacct ggtcttctgg agcagggcgg gcagctggcg ctactcggcg cgggcgatcc
     8641 ggtgctgcag gaaggtttcc ttgcggcggc agcggaatac cccggccagg tgggcgttca
     8701 gattggctat cacgaagcat tttcgcatcg cattatgggc ggcgcggacg tcattctggt
     8761 gcccagccgt tttgaaccgt gcggcttaac gcaactttat ggattgaagt acggtacgct
     8821 gccgttagtg cggcgcaccg gtgggcttgc tgatacggtt tctgactgtt ctcttgagaa
     8881 ccttgcagat ggcgtcgcca gtgggtttgt ctttgaagat agtaatgcct ggtcgctgtt
     8941 acgggctatt cgacgtgctt ttgtactgtg gtcccgtcct tcactgtggc ggtttgtgca
     9001 acgtcaggct atggcaatgg attttagctg gcaggtcgcg gcgaagtcgt accgtgagct
     9061 ttactatcgc ttgaaatagt tttcaggaaa cgcctatatg aatgctccgt ttacatattc
     9121 atcgcccacg cttagcgtag aagctcttaa gcactctatc gcttacaagc tgatgtttac
     9181 gattggcaag gatccggtcg tcgccaataa acatgaatgg ctgaacgcaa cgttatttgc
     9241 tgtgcgcgat cgtctcgtgg agcgctggtt acgctcaaac cgtgcccagt tgtcgcaaga
     9301 aactcgtcag gtttactacc tgtcgatgga gtttttgatt ggccgtacgc tttccaacgc
     9361 catgttgtcg ctaggaattt acgaagatgt acagggcgca ctggaagcga tgggattaaa
     9421 tctcgaagag ctgattgatg aagaaaatga cccgggcctc ggtaacggtg gcctgggccg
     9481 tctggcggct tgcttcctcg attctctggc gacgttaggg ttgccggggc gcggttacgg
     9541 catccgctat gactacggta tgttcaagca gaacatcgtt aacggtagcc agaaagagtc
     9601 gcctgactac tggctggaat acggtaaccc gtgggaattc aaacgccaca acacgcgcta
     9661 taaagtccgt tttggcggtc gcattcagca ggaaggtaaa aaaacgcgct ggattgaaac
     9721 cgaagagatt ctgggagtag cttacgatca gataatccca ggttacgaca ccgacgcgac
     9781 caacacgttg cgtttgtgga gtgcccaagc cagtagcgaa attaacctcg gtaaattcaa
     9841 ccagggcgac tacttcgcgg cagtggaaga taaaaaccac tccgagaacg tatctcgcgt
     9901 actgtatccg gacgactcca cctactccgg gcgtgagctg cgactgcgtc aggaatactt
     9961 cctggtttcc tcgaccattc aggacatttt aagccgccat tatcagttgc ataaaaccta
    10021 cgataacctg gcggataaaa ttgcgattca cctcaacgat actcatccgg tgctgtcgat
    10081 tcctgagatg atgcgtctgc tgatcgatga gcaccaattt agctgggacg acgcgtttga
    10141 agtgtgttgt caggtcttct cctacaccaa ccacacgcta atgagcgagg cgctggaaac
    10201 ctggccagtc gatatgctgg gtaaaattct gccgcgtcac ctgcagatca tctttgaaat
    10261 caacgactat ttcctgaaaa ccttgcagga acagtatccg aacgataccg atctgctggg
    10321 acgggcgtcg atcattgatg aatccaacgg tcgtcgtgtg cgtatggcct ggctggcggt
    10381 tgtggtgagc cacaaagtta acggtgtatc ggagctgcac tctaacctga tggtgcaatc
    10441 gctatttgcc gactttgcga aaatcttccc gggtcgtttc accaacgtca ccaacggtgt
    10501 gacgccgcgt cgctggctgg cggtagcgaa cccatcgctt tcagccgtgt tggacgaaca
    10561 cctgggccgc aactggcgca ccgaccttag cctgcttaat gatctgcaac aacactgtga
    10621 tttcccaatg gttaatcacg cggtgcatca ggcgaagctg gagaacaaaa agcgtctggc
    10681 ggagtatatc gcccagcagc tgaatgtggt ggtaaatccg aaagcgctgt tcgatgtgca
    10741 aatcaaacgt attcacgaat acaaacgtca attgatgaat gtgttgcacg tgatcacccg
    10801 ctataaccgc atcaaggccg acccggatgc gaagtgggta ccgcgcgtga atatttttgg
    10861 tggtaaggcg gcttcggcct attacatggc gaagcacatt attcatttga tcaatgacgt
    10921 agcgaaagtg atcaacaacg atccgcagat tggcgacaag ctgaaagtcg tgttcatccc
    10981 gaactacagt gttagcctgg cgcagttgat cattccggcg gccgatctgt ctgaacagat
    11041 ttcgctggcg gggacggaag cttccggcac cagtaacatg aagtttgcgc ttaacggcgc
    11101 gctgactatc ggtacgctgg acggtgcgaa tgtcgagatg ctggatcatg tcggtgctga
    11161 caatatcttt atctttggta acacagcgga agaagtggaa gaactgcgtc gtcagggcta
    11221 caaaccgcgt gaatactacg agaaagatga ggagctgcat caggtgctga cgcaaatcgg
    11281 cagcggtgta ttcagtccgg aagatccggg tcgctatcgc gatctggtcg attcgctgat
    11341 caacttcggc gatcactacc aggtactggc ggattatcgc agctatgtcg attgtcagga
    11401 taaagtcgac gaactctacg agcgtcagga agagtggacc gcaaaagcga tgctgaacat
    11461 tgccaatatg ggctacttct cttctgaccg tacgatcaaa gagtacgccg atcatatctg
    11521 gcatattgat ccggtgagat tgtaagttca aatatggcta aataccgtgt gttccgctga
    11581 agtaatacag agtggaactc acggtatttc tatacacaat aaac
//
LOCUS       DAZGHF010000144        11451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGHF010000144 DAZGHF010000000
VERSION     DAZGHF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.145"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            240..539
                     /gene="yoaC"
                     /locus_tag="ACHK3A_004132"
     CDS             240..539
                     /gene="yoaC"
                     /locus_tag="ACHK3A_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO8742143.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(659..838)
                     /gene="yoaH"
                     /locus_tag="ACHK3A_004133"
     CDS             complement(659..838)
                     /gene="yoaH"
                     /locus_tag="ACHK3A_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO8742144.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HAGERIVARFEDEDE"
     gene            912..2273
                     /gene="pabB"
                     /locus_tag="ACHK3A_004134"
     CDS             912..2273
                     /gene="pabB"
                     /locus_tag="ACHK3A_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO8742145.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSENEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEGFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHTSDLLRAAFPGGSITGAPKVRAMEIIDELEPHRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2276..2854
                     /gene="nudL"
                     /locus_tag="ACHK3A_004135"
     CDS             2276..2854
                     /gene="nudL"
                     /locus_tag="ACHK3A_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO8742146.1"
                     /translation="MEHRSLTLDDFLSRFQLLRPQMNRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3038..4402
                     /gene="sdaA"
                     /locus_tag="ACHK3A_004136"
     CDS             3038..4402
                     /gene="sdaA"
                     /locus_tag="ACHK3A_004136"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO8742147.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4533..6131
                     /gene="pdeD"
                     /locus_tag="ACHK3A_004137"
     CDS             4533..6131
                     /gene="pdeD"
                     /locus_tag="ACHK3A_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8742148.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDHALIKGSPVLIQWTPAAGSSK
                     AGVMEMINIDLLAAMLLEPQLPQISSASLTVDDRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLLRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6135..7691)
                     /gene="yoaE"
                     /locus_tag="ACHK3A_004138"
     CDS             complement(6135..7691)
                     /gene="yoaE"
                     /locus_tag="ACHK3A_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO8742149.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7679..7837)
                     /gene="yoaL"
                     /locus_tag="ACHK3A_004139"
     CDS             complement(7679..7837)
                     /gene="yoaL"
                     /locus_tag="ACHK3A_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO8742150.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8154..9125
                     /gene="manX"
                     /locus_tag="ACHK3A_004140"
     CDS             8154..9125
                     /gene="manX"
                     /locus_tag="ACHK3A_004140"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO8742151.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKATPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9188..9988
                     /gene="manY"
                     /locus_tag="ACHK3A_004141"
     CDS             9188..9988
                     /gene="manY"
                     /locus_tag="ACHK3A_004141"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO8742152.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10001..10852
                     /gene="manZ"
                     /locus_tag="ACHK3A_004142"
     CDS             10001..10852
                     /gene="manZ"
                     /locus_tag="ACHK3A_004142"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO8742153.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10907..11365
                     /gene="yobD"
                     /locus_tag="ACHK3A_004143"
     CDS             10907..11365
                     /gene="yobD"
                     /locus_tag="ACHK3A_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO8742154.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2808 a         2889 c         2864 g         2890 t
ORIGIN      
        1 cgcccttcga ttagcatctc tggttttctt tcttacctga acaatctggt tatagcgtga
       61 ggactgacca ttaattttct ccatcttact ggataacaca ctatgagctg ctacctttaa
      121 aacacctgaa tgtcaacagg ttaactcgtg ccatatcgtt ataatccctt tgatattaga
      181 tacaaattaa ggtcatatat agccttatcg ataacatggt taattttaag gataagaata
      241 tgcctgctgt aatagataaa gccctggatt tcattggtgc catggatgta tcagcgccaa
      301 caccaagttc gatgaatgaa agtacggcga agggaatctt taaatattta aaagaactgg
      361 gagtacccgc gagtgccgct gatattacgg cgcgagctga ccaggaaggc tggaaccctg
      421 gtttcacgga aaaaatggtt ggatgggcaa aaaaaatgga gtctggtgaa cgctctgtga
      481 ttaaaaatcc tgaatacttt tcaacatata tgcaggaaga actaaaagca ctggtttgag
      541 ttaaatttac atcagcataa atgggtcagg acgcttttaa tcacatataa aaagcgtcct
      601 ttttctcaca atcaaataat catcggcttc tataacgaaa gcgatacgga ttagtgtgtt
      661 actcgtcttc atcctcaaaa cgcgccacga ttcgctcgcc ggcatggttg gcacgcagct
      721 cttctgccac caacgcgatt gcctggccgc tgctcatccc ttgagccatc agttcctgga
      781 tccgctcgac agctttttgc tgctgttcat gggtgagtga aggtaaacct gcaaacattg
      841 ttaactcctg ctaaattgtt ggcgctaatt atttcatgct acccggcaca tagccagtag
      901 agtcaggact gatgaagacg ttatctcccg ctgtgattac tttaccctgg cgtcaggacg
      961 ccgctgaatt ttatttctca cgcttaagcc acctgccgtg ggcgatgctt ttacactccg
     1021 gctatgctga tcatccatat agccgctttg atattgtggt cgccgagccg atttgcactt
     1081 taaccacttt cggtaaagaa accgttgtta gtgaaaacga aaaacgcaca acgaccactg
     1141 atgacccgct acaggtgctc cagcaggtgc tggatcgcgc agacattcgc ccagcgcata
     1201 acgaagattt gccatttcag ggcggcgcgc tggggttgtt tggctacgat ctgggccgcc
     1261 gttttgagtc actgccagaa attgcgcagc aagatatcgt tctgccggat atggcagtgg
     1321 gtatctacga ctgggcgctg gttgttgacc accagcgtca aacagtttct ttgctgagtc
     1381 ataatgatgt caatgctcgt cgggcctggc tggaaagcca gcaattctcg ccgcaggaag
     1441 gtttcacgct cacttccgac tggcaatcca atatgacccg cgagcagtac ggcgaaaaat
     1501 ttcgccaggt acaggaatat ctgcacagcg gtgattgcta tcaggtgaat ctcgcccagc
     1561 gttttcatgc gacctattct ggcgatgaat ggcaggcatt ccttcagctt aatcaggcca
     1621 accgcgcgcc atttagcgct tttttacgtc ttgaacaggg tgcaatttta agcctttcgc
     1681 cagagcggtt tattctttgt gataatagtg aaatccagac ccgcccgatt aaaggcacgc
     1741 taccacgcct gcccgatcct caggaagata gcaaacaagc agaaaaactg gcgaactcag
     1801 cgaaagatcg tgccgaaaat ctgatgattg tcgatttaat gcgtaatgat atcggtcgcg
     1861 ttgccgtagc cggttcggta aaagtaccag agctgttcgt ggtcgaaccc ttccctgccg
     1921 tgcatcatct ggtcagcacc ataacggcgc ggctaccaga acagttacac accagcgatc
     1981 tgctgcgcgc agcttttcct ggtggctcaa taactggggc tccgaaagta cgggctatgg
     2041 aaattatcga cgaactggaa ccgcaccgac gcaatgcctg gtgcggcagc attggctatt
     2101 tgagcttttg cggcaacatg gataccagca ttactatccg cacgctaact gccattaacg
     2161 gacaaatata ctgctctgcg ggcggtggaa ttgtcgccga tagccaggaa gaagcggaat
     2221 atcaggaaac ttttgataaa gttaataaga tcctacgtca actggagaaa taaacgtgga
     2281 acaccgtagc ctgacgcttg atgatttttt atcgcgcttt caacttttgc gtccgcaaat
     2341 gaaccgggaa acgttaaatc atcgtcaggc tgccgtgtta atccccatcg tccgtcgacc
     2401 gcaaccgggg ttgttgctga ctcagcgttc gattcatctg cgtaaacacg ctggacaagt
     2461 ggcattccct ggaggcgcag tcgatgacac ggacgcatca gttatcgccg ccgcgctgcg
     2521 cgaagctgaa gaagaggtcg ctataccgcc ttccgccgtt gaagttatcg gcgtgctgcc
     2581 gcccgtcgat agcgtaactg gctaccaggt aaccccagtg gtcggcatta tcccgcccga
     2641 tctgccgtat cgcgccagtg aagatgaagt ctcggcggtg tttgaaatgc cgctcgccca
     2701 ggcattacat ctgggtcgtt atcatccttt agatatctac cgccgtggcg attcacatcg
     2761 ggtatggctg tcctggtacg aacagtattt tgtatgggga atgaccgcag gcataattcg
     2821 tgagctggcg ctgcaaattg gtgtgaaacc ctgactatac ttatctttac atctacaaaa
     2881 cactacttga gacaatcatc gcaatattag ttaaatcgcg gtttttgatt agtttaattc
     2941 atgtgaatag ttaagccagt cgccgcgttc cctcttacac tatgcgctgt tattagttcg
     3001 ttactggaag tccagtcacc ttgtcaggag tattatcgtg attagtctat tcgacatgtt
     3061 taaggtgggg attggtccct catcttccca taccgtaggg cctatgaagg cgggtaaaca
     3121 gttcgtcgat gatctggtcg aaaaaggctt actggatagc gttactcgcg ttgccgtgga
     3181 cgtttatggt tcactgtcgc tgacgggtaa aggccaccac accgatatcg ccattattat
     3241 gggtcttgca ggtaacgaac ctgccaccgt ggatatcgac agtattcccg gttttattcg
     3301 cgacgtagaa gagcgcgaac gtctgctgct ggcacaggga cggcatgaag tggatttccc
     3361 gcgcgacaac gggatgcgtt ttcataacgg taaccttccg ctgcatgaaa acggtatgca
     3421 aatccacgcc tataacggcg atgaagtcgt ctacagcaaa acttattatt ccatcggcgg
     3481 cggttttatc gtcgatgaag aacactttgg tcaggatgct gccaacgaag tgagcgtgcc
     3541 gtatccgttc aaatctgcca ccgaactgct cgcgtactgt aatgaaaccg gctattcgct
     3601 ttctggtctc gctatgcaaa acgaactggc gctgcacagc aagaaagaga tcgacgagta
     3661 tttcgcgcat gtctggcaaa ccatgcaggc atgtatcgat cgcgggatga ataccgaagg
     3721 cgtactgcca ggcccgttgc gcgtgccacg tcgtgcgtcg gccctgcgcc ggatgctggt
     3781 ttccagcgat aaactgtcta acgatccgat gaatgtcatt gactgggtaa acatgtttgc
     3841 gctggcagtt aacgaagaaa acgccgccgg tggccgtgtg gtaactgcgc caactaacgg
     3901 tgcctgcggt atcgttccgg cagtgctggc ttactatgac cactttattg aatcagttag
     3961 cccagacatc tatacccgtt actttatggc agcgggcgcg attggtgcac tgtataaaat
     4021 gaacgcgtct atttccggtg cggaagtcgg ttgccagggc gaagtaggtg ttgcctgttc
     4081 aatggctgct gcgggccttg ccgagctgtt gggcggtagc ccggaacagg tttgtgtggc
     4141 agcggaaatt ggcatggagc acaacctcgg tctgacctgc gaccccgttg cagggcaagt
     4201 tcaggtgccg tgcattgaac gtaatgccat tgcctctgtg aaggcgatta acgccgcacg
     4261 gatggctctg cgccgcacca gtgcaccgcg cgtctcgctg gataaggtca tcgaaacgat
     4321 gtacgaaacc ggtaaggaca tgaacgctaa ataccgcgaa acctcacgcg gtggtctggc
     4381 aatcaaagtc cagtgtgact aatacttctt actcgcccat ctgcaacgga tgggcgaatt
     4441 tattcccgct ttctcgtctg ctgtaatttt ccccactaca cttccactgt tgcgtcaggc
     4501 gtttgtcgcc atacgcttac agggtggccc gcatgcaaaa agcacaacgg atcattaaaa
     4561 cctatcgccg taatcgaatg attgtttgta cgatttgcgc actcgttacg ctcgcttcga
     4621 ccctgagcgt gcgatttatt tcacagcgta acttaaatca acaacgggta gtacaattcg
     4681 ccaatcacgc tgtagaggaa ttagataaag tactgcttcc cctacaggca ggtagcgaag
     4741 tcttgcttcc gctgattggt ctgccctgct ctgtcgccca tttgccatta cgtaaacagg
     4801 cggcaaaact ccaaactgtg cgatccattg gcctggtgca agacggcaca ctttattgct
     4861 ccagcatttt tggttatcgc aatgtgcccg tcgtggacat tctggctgaa cttcctgcac
     4921 cgcaaccact tttacgcctg acgaccgacc atgccctgat taaaggcagt ccggttttga
     4981 ttcaatggac gccagcagcg ggcagtagca aagctggggt catggagatg attaacatcg
     5041 acttactggc ggcaatgctg cttgagccac aactgccgca aatcagtagc gccagcctga
     5101 cggtggacga tcggcatttg ctctatggta atgggctggt agattccctt ccgcaacctg
     5161 aaaacaatga aaactatcag gtttcttcgc aacgctttcc ttttaccatt aacgttaatg
     5221 gtccgggggc tacggcgctg gcatggcact atcttccaac acaattaccg ctggcggtgc
     5281 tgctaagttt acttgtgggc tatatcgcct ggctggcgac cgcttaccgg atgagttttt
     5341 cccgcgaaat caatctgggc ctggcgcaac atgagttcga attgttctgc cagcctttgc
     5401 ttaatgcgcg cagccagcaa tgtattggcg tagagatttt gctgcgctgg aacaatccgc
     5461 gtcagggctg gatttcgccg gatgtgttta ttcctatcgc ggaagaacat catttaattg
     5521 tgccactgac ccgctatgtg atggcagaaa ccattcgtca gcgccatgtt ttcccgatga
     5581 gtagtcagtt tcatgttggc attaacgtcg cacccagcca ttttcgccgt ggtgtgctga
     5641 taaaagatct caatcagtac tggtttagcg ctcacccgat tcagcaactg atcctcgaaa
     5701 tcaccgaacg cgatgcctta ctggatgttg attatcggat tgcccgcgag ctacatcgta
     5761 aaaacgtcaa actggcgatt gatgacttcg gcaccggcaa tagttcattt tcctggcttg
     5821 aaacattacg tcctgacgtg ctgaaaattg ataagtcatt taccgcagct ataggttctg
     5881 acgcggttaa ctcgacggtg accgatatca tcatcgcact ggggcaaaga ctgaatattg
     5941 aactggtggc ggagggtgtg gaaacgcaag aacaggcgaa atatttgctc cgtcatggcg
     6001 tccatatttt gcaagggtat ttgtacgcac agccgatgcc actacgtgat tttcccaaat
     6061 ggctggcggg cagccaaccg ccgcccgccc ggcataatgg acatatcaca cccattatgc
     6121 cgttacgtta acgcttactc atcttcatcg tgcgccggtt gctctttaac aatgcgaacc
     6181 agatcaacac gataatcatt ggcttcaatg atggtgatat gcagtggccc tacatcaatc
     6241 acatcgccca cacggggaat gtgaccattt gccgagatca cgaggcccgc gaccgtcgcg
     6301 atatcatcgt catcggcaag gtgctcaaca tcaagcgcct gctgcaaggc atgcaaatct
     6361 gtaccgcctt ttaccagcca gccgtcaccg tcggtaatga tttccggcgt ttcgtcagcg
     6421 tccgggaatt cacccgcaat ggcttccagc acatccagcg gcgtgaccaa gccttgtacc
     6481 acaccaaact cgttggtcac gataacaaag ctcccgcgag cacgacgcag cacgcccagc
     6541 aggttgatcg gatcgagggt ttccgggacg ataatcgccg gagacgccga agcaatcgcc
     6601 gccacatcaa cgccctcttc cagcgccacc agcagttctt tagcacgtac gattccgatg
     6661 atttcatcca gttcaccgcg acataccggg aacaggctgt gcggtgagga gagcagctgc
     6721 tcgcggattt catctacccc gagattagcg tcaacccagc ttatttcacc gcgcggcgtc
     6781 atgatcccgc gcagagaacg cgacgccagc gtcagtacgc cgttaatcat gtaacgttct
     6841 tcttcggcaa atgcaccttc cgggatcggc atcggcatcg ggttatcggc atcgtgctga
     6901 acattggcct gacgtttccc gcccatcaaa cgcaggatgg catcggcagt acgcgctcgc
     6961 agcggcaaag tcgactggtg acgaataaag ttgcgacgcg caatctggtt aaacacttcg
     7021 atgatgatcg agaagccaat cgcggcatac aggtaacctt tcggaatgtg gaaaccgaaa
     7081 ccttctgcca ccagactcag accaatcatt aacaggaagc tcagacagag caccaccacc
     7141 gtggggtgct ggttaacaaa tcgcgtcagc ggtttcgatg ccagcagcat aactgccatc
     7201 gcaatcacta ccgccgccat catcaccggc aaatggttaa ccatccctac tgcagtaatt
     7261 accgcatcca acgagaagac ggcgtcaagg atgacaatct gtgtgacgac cacccagaaa
     7321 ctggcgtagc ctttaccgtg gccggaatca tgatcgcggt tttccagccg ttcatgcagt
     7381 tcggttgttg ctttgaacag caagaatatg cccccgaaca acataatcag gtcgcgtccg
     7441 gagaaggaga aatccatgac ggtaaatagc ggtttggtca gcgtgaccat ccatgaaatc
     7501 agcgacagca gccccagacg cataatcagc gccagtgata atcccagcaa acgcgcttta
     7561 tcgcgttgtt ttggcggcag tttgtcagca agaatggcaa tgaagaccag gttatcgata
     7621 cccagcacaa tttcgagaac aacaagcgtg agtagccccg cccaaattga ggggtccatt
     7681 aagaattcca tgacaagctc ctgcttaagg aatagctatt cgacgccaga aataatgcag
     7741 gcgtaacgac aaaatgcaaa cgaaaggtgc ggcatagagt gccagaaagg caggcgttaa
     7801 aaggcctgat gctgaaatga cgtcggtgac gatccatact gcgggctact gccctatact
     7861 ccatggttgt taaacgggag ttaaacatat cagagacgcc tctgatttgg caaagattta
     7921 ccttcctttg caaacgaatg tgacaaggat attttacctt tcgaaatttc tgctaatcga
     7981 aagttaaatt acggatcttc atcacataaa ataatttttt cgatatctaa aataaatcgc
     8041 gaaacgcagg ggtttttggt tgtagccctt atctgaatcg attcgattgt ggacgacgat
     8101 tcaaaaatac atctggcacg ttgaggtgtt aacgataata aaggaggtag caagtgacca
     8161 ttgctattgt tataggcaca catggttggg ctgcagagca gttgcttaaa acggcagaaa
     8221 tgctgttagg cgagcaggaa aacgtcggct ggatcgattt cgttccaggt gaaaatgccg
     8281 aaacgctgat tgaaaagtac aacgctcagt tggcaaaact cgacaccact aaaggcgtgc
     8341 tgtttctcgt tgatacatgg ggaggcagcc cgttcaatgc tgccagccgc attgtcgtcg
     8401 acaaagagca ttatgaagtc attgcaggcg ttaacattcc aatgctcgtg gaaacgttaa
     8461 tggcccgtga tgatgaccca agctttgatg aactggtggc gctggcagta gaaacaggtc
     8521 gtgaaggcgt gaaagcactg aaagccaaac cggttgaaaa agccgcgcca gcacccgctg
     8581 ccgcagcacc aaaagcgact ccaactccgg caaaaccaat gggaccaaac gactacatgg
     8641 ttattggcct tgcgcgtatc gacgaccgtc tgatacacgg tcaggtcgcc acccgctgga
     8701 ccaaagaaac caatgtctcc cgtattattg ttgttagtga tgaagtggct gcggataccg
     8761 ttcgtaagac actgctcacc caggttgcac ctccgggcgt aacagcacac gtagttgatg
     8821 ttgccaaaat gattcgcgtc tacaacaacc cgaaatatgc tggcgaacgc gtaatgctgt
     8881 tatttaccaa cccaacagat gtagagcgtc tcgttgaagg cggcgtgaaa atcacctctg
     8941 ttaacgtcgg tggtatggca ttccgtcagg gtaaaaccca ggtgaataac gcggtttcgg
     9001 ttgatgaaaa agatatcgag gcgttcaaga aactgaatgc gcgcggtatt gagctggaag
     9061 tccgtaaggt ttccaccgat ccgaaactga aaatgatgga tctgatcagc aaaatcgata
     9121 agtaacgtat tgtgttgatt atcactcagt tttcacactt aagtcttacg taaacaggag
     9181 aagtacaatg gagattacca ctcttcaaat tgtgctggta tttatcgtag cctgtatcgc
     9241 aggtatggga tcaatcctcg atgaatttca gtttcaccgt ccgctaatcg cgtgtaccct
     9301 ggtgggtatc gttcttggcg atatgaaaac cggtattatt atcggtggta cgctggaaat
     9361 gatcgcgctg ggctggatga acatcggtgc tgcagttgcg cctgacgccg ctctggcttc
     9421 catcatttct actattctgg ttatcgcagg tcatcagagc attggtgcag gtatcgcact
     9481 ggcaatccct ctggccgctg cgggccaggt actgaccatc atcgttcgta ctattaccgt
     9541 tgccttccag cacgctgcgg ataaggctgc tgataacggc aacctgacag cgatttcctg
     9601 gatccacgtt tcttctctgt tcctgcaagc aatgcgtgtg gctattccgg ccgtcatcgt
     9661 tgcgctgtct gttggtacca gcgaagtcca gaacatgctg aatgcgattc cggaagtggt
     9721 gaccaatggt ctgaatatcg ccggtggcat gatcgtggtg gttggttatg cgatggttat
     9781 caacatgatg cgtgctggct acctgatgcc gttcttctac ctcggcttcg taaccgcagc
     9841 attcaccaac tttaacctgg ttgctctggg tgtgattggt actgttatgg cagtgctcta
     9901 catccaactt agcccgaaat acaaccgcgt agccggtgcg cctgctcagg cagctggtaa
     9961 caacgatctc gataacgaac tggactaaca ggtgagcgaa atggttgata caactcaaac
    10021 taccaccgag aaaaaactca ctcaaagtga tattcgtggc gtcttcctgc gttctaacct
    10081 cttccagggt tcatggaact tcgaacgtat gcaggcactg ggtttctgct tctctatggt
    10141 accggcaatt cgtcgcctct accctgagaa caacgaagct cgtaaacaag ctattcgccg
    10201 tcacctggag ttctttaaca cccagccgtt cgtggctgcg ccgattctcg gcgtaaccct
    10261 ggcgctggaa gaacagcgtg ctaatggcgc agagatcgat gacggtgcta tcaacggtat
    10321 caaagtcggt ttgatggggc cgctggctgg tgtaggcgac ccgatcttct ggggaaccgt
    10381 acgtccggta tttgcagcac tgggggccgg tatcgcaatg agcggcagcc tgttaggtcc
    10441 gctgctgttc ttcatcttgt ttaacctggt gcgtctggca acccgttact acggcgtagc
    10501 gtatggttac tccaaaggta tcgatatcgt taaagatatg ggtggtggct tcctgcaaaa
    10561 actgacggaa ggggcgtcta tcctcggcct gtttgtcatg ggggcattag ttaacaagtg
    10621 gacgcatgtc aacatcccgc tggttgtctc tcgcattact gaccagacgg gcaaagaaca
    10681 cgttaccact gtccagacta ttcttgacca gttaatgcca ggcctggtac cactgctgct
    10741 gacctttgct tgtatgtggc tgttgcgcaa aaaagttaac ccgctgtgga tcatcgttgg
    10801 cttcttcgtc atcggtatcg ctggttacgc ttgcggcctg ctgggactgt aagactgttg
    10861 tacactaccg gggccttttg gccccgtttt tttatctgga ggattaatga caatcacgga
    10921 cctggtactg attcttttca tcgccgcact cctggccttc gcgatctacg atcagttcat
    10981 catgccccgc cgtaacggcc ccaccctgct ggcaattcct ttgctccggc gtggtcgcat
    11041 cgatagcgtt atcttcgtcg gattgattgt cattcttatc tataacaacg tcacgaatca
    11101 tggcgcgcta ataacaacat ggttattaag tgcactggct ctgatgggtt tttatatatt
    11161 ctggatccgc gttccgaaga tcatctttaa acaaaaaggt tttttcttcg ccaatgtctg
    11221 gattgaatat agccgaatca aagcgatgaa cttgtcggaa gatggcgtgt tggtgatgca
    11281 attagaacag cgccggctgt taatccgcgt tcgaaatatc gacgatctgg aaaaaattta
    11341 taagcttctc gtttcaactc aataagttat gaatttagcc aaagctatgt ttagtgtatt
    11401 tttaataatc agacatagct taggctatat tacctcttcc cttatttgtt a
//
LOCUS       DAZGHF010000145        11396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGHF010000145 DAZGHF010000000
VERSION     DAZGHF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.146"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            15..2438
                     /gene="pgaA"
                     /locus_tag="ACHK3A_004144"
     CDS             15..2438
                     /gene="pgaA"
                     /locus_tag="ACHK3A_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO8742155.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYSRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLKS
                     GAPDKANLLAEAYIYKLTGRHQDELRAMTESLPENASKQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELIRLSFMPTRSESERYAIADRALAQYAALEILWHDNSDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKNTRQIIPPWAQYWVASAYLKEQQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMIARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLVELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRQEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2447..4465
                     /gene="pgaB"
                     /locus_tag="ACHK3A_004145"
     CDS             2447..4465
                     /gene="pgaB"
                     /locus_tag="ACHK3A_004145"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO8742156.1"
                     /translation="MLRNGNKYILMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYIYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLTQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4458..5783
                     /gene="pgaC"
                     /locus_tag="ACHK3A_004146"
     CDS             4458..5783
                     /gene="pgaC"
                     /locus_tag="ACHK3A_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO8742157.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5785..6198
                     /gene="pgaD"
                     /locus_tag="ACHK3A_004147"
     CDS             5785..6198
                     /gene="pgaD"
                     /locus_tag="ACHK3A_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO8742158.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFKKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(6248..7036)
                     /gene="phoH"
                     /locus_tag="ACHK3A_004148"
     CDS             complement(6248..7036)
                     /gene="phoH"
                     /locus_tag="ACHK3A_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO8742159.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIIRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7662..8933)
                     /gene="efeB"
                     /locus_tag="ACHK3A_004149"
     CDS             complement(7662..8933)
                     /gene="efeB"
                     /locus_tag="ACHK3A_004149"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO8742160.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGVISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(8939..10066)
                     /gene="efeO"
                     /locus_tag="ACHK3A_004150"
     CDS             complement(8939..10066)
                     /gene="efeO"
                     /locus_tag="ACHK3A_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO8742161.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKVNPELLAKVDANFIKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10124..10954)
                     /gene="efeU"
                     /locus_tag="ACHK3A_004151"
     CDS             complement(10124..10954)
                     /gene="efeU"
                     /locus_tag="ACHK3A_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO8742162.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSVFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3006 a         2816 c         2692 g         2882 t
ORIGIN      
        1 tggagtaata caggatgtat tcaagtagca gaaaaaggtg cccgaaaacc aaatgggctt
       61 tgaaacttct tactgccgca tttttagcag cgagtcccgc ggcgaagagt gctgttaata
      121 acgcctatga tgcattgatt attgaagctc gcaaaggaaa tactcagcca gctttgtcat
      181 ggtttgcaca aaaatcaaca ctcagtaata accaaattgc tgactggtta caaattgcct
      241 tatgggccgg gcaagataaa caggtcatta ccgtttacag ccgctaccgt catcagcaat
      301 taccagcgcg tggttatgca gctgtcgccg tcgcttatcg taacctgcaa caatggcaaa
      361 actcgcttac actgtggcaa aaggcgctct ctctggagcc gcaaaataag gattatcaac
      421 ggggacaaat tttaaccctg gcagatgcgg gtcactatga tactgcgctg gttaaactta
      481 agcagcttaa atctggagcg ccggacaaag ccaatttact cgcagaagcc tatatctata
      541 aactgacggg gcgtcatcag gatgaattac gggcgatgac agagtcatta cctgaaaatg
      601 catctaagca acaatatccc acagaatacg tgcaggcatt acgtaataat caactagctg
      661 ccgcgattga cgatgccaat ttaacgccag atattcgcgc tgatattcat gccgaactga
      721 tcagactgtc gtttatgcct acgcgcagtg aaagtgaacg ttatgccatt gccgatcgcg
      781 ccctcgccca atacgctgca ttagaaattc tgtggcacga taactcagac cgcactgccc
      841 agtaccagcg tattcaggtt gatcatcttg gcgcgttatt aactcgcgat cgttataaag
      901 acgttatttc tcactatcag cgtttaaaaa atacgaggca aattattccg ccctgggcgc
      961 aatattgggt tgcgtcggct tatctcaaag aacagcagcc aaaaaaagca cagtcaataa
     1021 tgacagagct cttttatcac aaggagacca ttgccccgga tttatccgat gaagaacttg
     1081 cggatctctt ttacagccac ctggagagtg aaaattatcc cggcgcgcta actgtcaccc
     1141 aacataccat taatacttcg ccgcctttcc ttcggttaat gggcacgcct acgagcatcc
     1201 cgaatgatac ctggttacag gggcattcgt ttctctcaac cgtagcaaaa tatagtaatg
     1261 atcttcctca ggctgaaatg atagccagag agcttgctta taacgcacca ggaaatcagg
     1321 gactgcgcat tgattacgcg agtgtgttac aagcccgcgg ttggcctcgt gcagcagaaa
     1381 atgaattaaa aaaagcagaa gtgatcgagc cacgtaatat taatctggag gttgaacaag
     1441 catggacagc attaacgtta caagaatggc agcaggcagc tgtcttaacg cacgatgttg
     1501 tcgaacgtga accgcaagat cccggcgttg tacgtttaaa acgtgcggtt gatgtacata
     1561 atcttgtaga gcttcgtata gctggttcaa caggaattga tgccgaaggc ccggatagtg
     1621 gtaaacatga tgtcgactta accaccatcg tttattcacc accgctgaag gataactggc
     1681 gcggttttgc tggattcggt tatgccgatg gacaatttag cgaaggaaaa gggattgttc
     1741 gcgactggct ggcgggtgtt gagtggcggt cacgtaatat ctggctcgag gcggagtacg
     1801 ctgaacgcgt tttcaatcat gagcataaac ccggcgcgcg cctgtctggc tggtatgatt
     1861 ttaatgataa ctggcgtatt ggttcgcaac tggaacgcct ctctcaccgc gttccattac
     1921 gggcaatgaa aaatggtgtt acaggcaaca gtgctcaggc ttatgttcgc tggtatcaaa
     1981 atgagcggcg taagtacggt gtctcctggg ctttcactga tttttccgac agtaaccagc
     2041 gtcaggaagt ctcacttgag ggtcaggaac gcatctggtc ttcaccatat ttgattgtcg
     2101 atttcctacc caatctgtat tacgaacaaa atacagaaca cgacactcca tactacaacc
     2161 ctataaaaac gttcgatatt gttccggcat ttgaggcaag tcatttgtta tggcgaagct
     2221 atgaaaatag ctgggagcaa atcttcagcg caggtgttgg tgcctcctgg caaaaacatt
     2281 atggcacgga tgtcgtcacc caactcggct acgggcaacg tattagctgg aatgacgtga
     2341 ttgatgctgg cgcaacgcta cgctgggaaa aacgacctta tgacggtgac agagaacaca
     2401 acttatacgt tgaattcgat atgacattca gattttaagg ataaatatgt tacgtaatgg
     2461 aaataaatat atcctgatgc tggtgagtat aattatgctc actgcgtgca ttagccagtc
     2521 aagaacatca tttataccgc cacaggatcg cgaatcttta ctcgccgagc aaccgtggcc
     2581 gcataatggt tttgtggcga tttcatggca taacgttgaa gacgaagctg ccgaccagcg
     2641 ttttatgtca gtgcggacat cagcactgcg tgaacaattt gcctggctgc gcgagaacgg
     2701 ttatcaaccg gtcagtattg ctcaaattcg tgaagcacat cgaggaggaa aaccgctacc
     2761 ggaaaaagca gtagtgctga cttttgatga cggctaccag agtttttata cccgcgtctt
     2821 cccaattctt caggccttcc agtggcctgc tgtatgggcc cccgtcggca gttgggtcga
     2881 tacgccagcg gatgaacaag taaaatttgg cgatgagatg gtcagtcgag aatattttgc
     2941 cacgtggcaa caagtgcgag aagttgcgcg ttcccggctc gttgaggtcg cttctcatac
     3001 atggaattct cactacggta ttcaggctaa tgccaccggc agcttattgc ctgtatatgt
     3061 aaatcgtgca tattttactg accacgcacg gtatgaaacc gcagcagaat accgggaaag
     3121 aattcgtctg gatgctgtaa aaatgacgga atacctgcgt acaaaggctg aggtaaatcc
     3181 acacgttttt gtttggcctt atggcgaagc gaatggcata gcaatcgcgg aattaaaaaa
     3241 actcggttat gacatgttct tcacccttga atcaggtttg gcaaatgcgt cgcaattaga
     3301 ttccattccg cgagtattaa tcgccaataa tccctcatta aaagagtttg cccagcaaat
     3361 tattaccgta caggaaaaac cactacaacg ggtaatgcat atcgatcttg attacattta
     3421 tgacgaaaac cgccagcaaa tggatcgcaa tattgatgtg ctaattcaga gggtaaaaga
     3481 tatgcaaata tctaccgtgt atttacaagc atttgctgat cccgatggtg atgggctggt
     3541 taaagaggtc tggtttccaa atcgtttgct accaatgaaa gcagatattt ttagtcgggt
     3601 tgcctggcaa ttgcgtaccc gctcaggtgt aaacatctat gcgtggatgc cggtattaag
     3661 ctgggattta gatcccacat taacgcgagt aaaatactta ccaactgggg agaaaaaagc
     3721 acaaattcat cctgaacaat accgccgtct ctcccctttc gatgacaggg tcagagcaca
     3781 agttggcatg ttatatgatg atcttgccgg acatgctgct tttgatggca tattgttcca
     3841 cgatgatgct ttgctttcag attatgaaga tgccagtgca gcggctatcg cggcttatca
     3901 gcaagctggc tttagcggga gtctgagcga aattcgacaa aacccggagc aattcaaaca
     3961 gtggacccgc tttaaaagtc gtgcgttaac tgacttcact ttagaactta gtgcgcgcgt
     4021 aaaagccatt cgtggtccac atgttaaaac tgcacgaaat atttttgcac ttccggtaat
     4081 acaacctgaa agtgaagctt ggtttgcaca gaattatgct gatttcctaa aaagctatga
     4141 ctggaccgct attatggcta tgccttatat ggaaggcgtc acagaaaaat cggctgatca
     4201 atggttaaca caattgacca atcagatgaa aagcatcccg caggctaaag acaaatcaat
     4261 tttagaatta caggcacaaa actggcagaa aaatggtcag catcaggcta tttcttcaca
     4321 acaactcgct cactggatga gcctattaca actgaatgga gtgaaaaatt atggttatta
     4381 tcccgacaat tttctgcata accaacctga aatagacgtt attcgtcctg agttttcaac
     4441 agcctggtat ccgaaaaatg attaatcgca tcgtatcgtt ttttatatta tgtctggtgt
     4501 tatgcatacc tctatgcgta gcgtactttc actctggtga actgatgatg aggttcgttt
     4561 tcttctggcc gttttttatg tccattatgt ggattattgg cggcgtctat ttctgggtct
     4621 atcgtgaacg ccactggccg tggggagaaa acgcaccagc cccccagttg aaagataatc
     4681 catctatctc cattatcatt ccctgtttta atgaggagaa aaacgttgag gaaaccatac
     4741 atgccgcttt agcacagcgt tatgagaaca ttgaagttat tgccgtaaat gatggttcaa
     4801 cagacaaaac ccgtgccatc ctggatcgca tggctgcaca aattccccgt ctgcgggtca
     4861 ttcatctggc gcaaaaccag gggaaagcca ttgcgcttaa aaccggagct gccgcggcga
     4921 aaagtgaata tctggtgtgc attgatggcg acgcgttatt agaccgcgat gcagcggcat
     4981 atattgtgga accgatgttg tacaacccac gtgtgggtgc cgtaaccggt aatcctcgta
     5041 ttcgcacacg ttctacccta gtgggtaaaa ttcaggttgg cgagtattcc tcaattattg
     5101 gtttgatcaa gcggacccag cgtatctatg gaaatgtatt taccgtttcc ggtgttattg
     5161 ccgcatttcg tcgcagcgcc ctggcagaag tgggttactg gagtgacgat atgatcaccg
     5221 aagatattga tattagctgg aagctgcagt tgaatcagtg gacgattttt tacgagccac
     5281 gggcgctgtg ctggatatta atgcctgaaa cgttaaaagg gctgtggaaa cagcgcctgc
     5341 gctgggctca gggcggtgca gaagtattcc tcaaaaatat gacaaggttg tggcgaaaag
     5401 aaaactttcg aatgtggcca ctgttttttg aatactgcct gacgacaata tgggccttta
     5461 cctgcctggt cggtttcatt atttacgcag tccaacttgc aggtgtaccg ttaaatattg
     5521 aattgacaca tatcgcagcg acacatactg ccggaatatt attgtgcacg ttatgtttac
     5581 tgcaatttat tgtcagtctg gtaattgaga atcgctatga gcacaatctg gcttcatcgc
     5641 ttttctggat tatttggttt ccggttattt tctggatgct aagcctggca acgacattgg
     5701 tatcatttac acgagtcatg ttgatgccta aaaaacaacg cgctcgttgg gtaagtcccg
     5761 atcgtgggat tctgagaggt taatatgaac aatctaatta ttacgacccg acaatcgcca
     5821 gtacgtttac tggttgatta tgttgccaca accatcttgt ggacattatt tgctttgttc
     5881 atattcttat tcgccattga tctgctgacg ggttattact ggcaaagcga ggccagaagc
     5941 cgacttcagt tctatttttt gctggcagtg gcgaatgccg tcgtgttaat cgtctgggca
     6001 ttgtacaata agctgcgttt taaaaaacag cagcatcatg cagcatacca atatacgccg
     6061 caagaatatg cagagagctt agcaatacct gatgagcttt atcagcaact acaaaaaagc
     6121 cacaggatga gcgtacactt caccagtcag ggacaaataa aaatggtggt ttcagaaaaa
     6181 acgctagtcc gggtataaac acccaaaaca aagcccggtt cgcccgggct ctgcaccgat
     6241 aatacactta actgtatgca tgtagcgtac gttggcaaag tgccgaacgt acgcaatcct
     6301 ctttaccgaa tcggatgatc ccgaccattt catcttcttc gaaacgttcc agcgcgtcac
     6361 ttaatccgga gcgcacaccg cgaggtaaat cgcattgcgt gatatcaccg ttgacgataa
     6421 ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca gtcacattct
     6481 gcgcctcgtc aagaatgacg actgcatttt caaaggtacg tccacgcata taggcgaacg
     6541 gcgcgatttc gaccttccct atttctggtc gcaggcagta ctgcataaag gaagccccta
     6601 agcgccggac cagcacgtcg taaaccgggc gaaaataggg agcaaacttt tctgcgatat
     6661 ctccaggtaa aaagccaaga tcttcatcgg cttgcagaac tggacgggtg acgataatcc
     6721 tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc caggtttttc
     6781 cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc ttcagatagt
     6841 gcaattgcgc ttcatttcgc gcgaggatgg gcgaggtatc gcgactgtcg cgggccatac
     6901 caatggcttc tacgccgctc atttgcacaa gcgaggtgac cgattcttct tcacgctgct
     6961 tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct ttgatcactg
     7021 ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac gcgccgttgg
     7081 cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca tagtttgctt
     7141 ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta acacgagtaa
     7201 agagaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga aatctccgcg
     7261 agtggtggag ccttgcttaa caaaacattg tccagaacag gacgttacca tccgcgatct
     7321 ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag tacaacagat
     7381 ctcgcattca ttgcaacaat atattcactt atatttaacc ataaaacacc atttcagtga
     7441 cattagtttc tactggaaag atgacaaagt gatgacagtg ataaaaaagc tgtgtgcttt
     7501 cagcaggatt cgcagtactg gtaaaagctt gccggatgcg gcgtgaacgc cttatccggc
     7561 ctaccgaacc cgcaccgatt gtaggcatga taagacgcgc aagcgtcgca tcagcgcacg
     7621 aacgcctggt gcggcgtaaa cgccttatcc gcctaaaaac attaaacccg caataatgcg
     7681 cttccgagat aatcgttcgc gtccttcaca cccggcagcg caaagaaata gccgccaccg
     7741 ataggtttga catattcctc cagcgcctct ccattcaacc ttttttgtac tgtcaggaag
     7801 cccttttcca gatcgtgttg gtagcagaca aacagtagcc ccatatcgag ttgcccggaa
     7861 ttggtgacgc ccaacgaata actgtagcca cgacgcagca tcagacttga ctcactctct
     7921 ggcgtgcggg gattcgccag ccggatatgg ctgtccagcg cgatcacctt cccctccgga
     7981 tcgctggcgt aatcaggcac atcatgctca tgctgcattc ccagcggcgc gccggtttgc
     8041 ttatcacgcc caaatatcgt ctgctgttct ttcagcggcg ttctgtccca aaattccacc
     8101 cgaaactgaa tcaaccgcac cgcctggtag ctaccaccga ttgtccacgc aggctcctgc
     8161 tgatctgccg tcacccacac cactttttgc atcaacttcg cattctggct atcgggattg
     8221 gcagtgccgt ctttgaaacc cagcaaatta atcggtgtct ctttaccttt actacgcgcc
     8281 gcgtggtcgg aaataacccc ttcccgcttc cagcgcacgc tgagcaaatc cggcgtgtgt
     8341 ttgatgatat cgcgcagcgc atggatcacc gtgtcctggg tgttggcgca aatttgtagc
     8401 aacacatcac catgacataa cgccgcatcc agcgagtcgt tggggaaacg cgtcatcttc
     8461 tgcaactttt tcggcatctg tggcgcaagg ccaaagcgct catcaaacaa tgagtggccc
     8521 accgataacg ttatggtgag attatcgggc gcaatgtagc cgccaagaat gccggaatcg
     8581 agtggtggca ggcgcggatt tggcgtttct ggtgccgctc cgccctgagt cagaaaagca
     8641 aaacgctgag tcaacaagcg aaacaaccgt tcaagatcgg ctttatcgct ggcaagcaca
     8701 tcaaacgcca ccagcatcat tgcagcctgt tgtggcgtca ggatccctgc ctgatgctca
     8761 ccataaaacg gctgtttctc attgcgtgca tccggtgaaa gcgtacccgg cgcactttgc
     8821 gttttttgtg catgagcgac cggacaactt cccgccagcg ccagcgcacc aatccctttc
     8881 agtaaacgtc ggcgtgacgg ttcattcacg ccgttttcat cttcatactg cataacgctt
     8941 agtccagccc cagcacaccg cgaagttgcg ccagatcttc cgccagcgca gtaatcggtc
     9001 ctttcagtgc attccggtca gcatcggtca gtttgtcgta attttcaaaa ccgtctttag
     9061 tacggtattt cgccagaatg gtatcgacct ttatgaagtt ggcatcgact tttgccaaca
     9121 gttccggatt cactttttgt agttgtggac gcagcagatc gacaattttc tgcgagcctt
     9181 caacgttagc ctggaaatcc cacagatcgg tgtggctgta gcgatcttct tcaccgctga
     9241 ttttgctggc cgccacttcc tcaatcagtc cggctgcacc gccgaccact tttgaaggag
     9301 ggaaagccag ttcactgatg cgtttttgca aatcgaccac atcggtataa agctggtcgg
     9361 catacttatc catccctttg gtggtgttat cgccaaacaa tgctttttcc agacggtgga
     9421 aaccggtgaa tttcggatcg gcggcttttt gctcgtaatc atcttcacgg gcgtcaatgc
     9481 tgccatccag atcggagaac agttcagcaa tcggttcaat gcgctcatag tgctggcgcg
     9541 tcggcgcata cagtgctttc gctttttcga tatcgcctgc tttaatagca tcggtaaagg
     9601 ctttggtgtc ggtcaccagc tgcgtggttt ccgccatgac atacgcttta tatgcagtaa
     9661 ttgcaccacc aagacttaac agcgcatcac tttgcgccgc atccgccgtt gcctcacctt
     9721 tgacgatcaa cttccctttc gggtttgtca gcagaccgca ggtcatatcg tattcaccag
     9781 gctgtaaatt tgccgtcatt ttctggctaa aaccaggggc gatattttcc cgctcttcca
     9841 ccaccatcac gcctttgagg atctcccact ccagtgcttt ctggctgtgg ttctgaataa
     9901 tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg ttcgcactgc ttatccgtca
     9961 cggtcacttt gacctgcggc acatcagcgg cgttagccat aaaagcagaa gaaaacagcg
    10021 cagccacgct caactgcaat gcgttacggc ggaagttaat ggtcatgacg agactatccc
    10081 tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat ttgttacgcg gagcgagacg
    10141 ctgtcgcccc tgcgcgtggt ggcagagcaa atgccaccag cgccgggatg agataaataa
    10201 accagacggc gacttcgctg acgctcggcg cttcctgata gccaaaaatc ccttccatca
    10261 gcgtgccaaa caccgagtga gttgagagca ccgcactcat atcgaaggcg atttcctgaa
    10321 agtggttcca caatccggct tcatgaaatg cgcgaatggc accagccgcc agccctgcgg
    10381 cgacgaagag aataaacagg ctggtccatt taaaaaatgc accaagattg aggcgaatac
    10441 cgccccagta gagcaggaag ccgagcacca cggctgtagc aagaccgagc attgcaccca
    10501 gcggcggcca gatcccgaca tcttgttgaa acgccgccag caggaaaaag accgactcca
    10561 gcccttccct tgcaacggca aaaaagacca tcatcaccag cgcccagcca tgatgatttc
    10621 cacgctgcaa tgcgctatcg actgcctgct ccagttgtac tttgacgttg cgcgacactt
    10681 tgcgcatcca gaaaaccatc caggtaagga tcaccacggc gatcaccgcc acgatacctt
    10741 caaacagttc ctgttctttt tgcggaaatt cgccggtggt ttcgttaatg aagataccca
    10801 agcccaggca caacgcagcg gcaagcaaca cgccaatcca catcacacca atccatcggc
    10861 ctcgctgggt acgcttaaga tagctggcaa tcaaactgac aatcagcgcg gcttcaagtc
    10921 cttcgcgcaa cataatgaga aacggaacaa acatgccacc cacccttaaa cgtttttctg
    10981 agtcaatcta tgcaaagaaa agtaaatcgc agtgatagcg attatcatta cggtaaagaa
    11041 aaatacaagc agaatgttta taaaatgatg ttggaatgta aacaaagaaa ggtaatgcca
    11101 ggggatataa acgattattc caggtacaaa gatgggattg tttacaacga gccatgtgtt
    11161 cttgccagat gccgcgtaaa tgccttatcc agccgacaaa actgtccaga ctcaatgcat
    11221 cagagcttcc atgtaaaact gataagcggg tgtatcaagc aatttcgtcc atccgcgatt
    11281 atcaaatcta actttttgcc cggaacataa taacagctcg ggtgttggtg gcgtcaaaat
    11341 cgctgaagcg ttccccgaag gccggggcag cccacatgga tgtgggctga gggcgc
//
LOCUS       DAZGHF010000146        11198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGHF010000146 DAZGHF010000000
VERSION     DAZGHF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.147"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(62..343)
                     /gene="yehE"
                     /locus_tag="ACHK3A_004152"
     CDS             complement(62..343)
                     /gene="yehE"
                     /locus_tag="ACHK3A_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO8742163.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(606..1715)
                     /gene="apbC"
                     /locus_tag="ACHK3A_004153"
     CDS             complement(606..1715)
                     /gene="apbC"
                     /locus_tag="ACHK3A_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO8742164.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPNMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1847..3880
                     /gene="metG"
                     /locus_tag="ACHK3A_004154"
     CDS             1847..3880
                     /gene="metG"
                     /locus_tag="ACHK3A_004154"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO8742165.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEEIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4022..7810
                     /locus_tag="ACHK3A_004155"
     CDS             4022..7810
                     /locus_tag="ACHK3A_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO8742166.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     AAAAEKAGLKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEISQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVTALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISHPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPSEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPEHAITGLLPAALGKASEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAVNALLALDALDNHPTKIPTLPTFYQPSLWTRPLLKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDETLKPFVRDASGSRLKDLPKPNKSDDELQANNAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTAD
                     DDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWAGRKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAV
                     GMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE
                     "
     gene            7820..>11198
                     /locus_tag="ACHK3A_004156"
     CDS             7820..>11198
                     /locus_tag="ACHK3A_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO8742167.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQHEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPWLSTQARAVLNSYLSAPPKTVIDSTDNSQMPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYPGRVEEIMNGWSSPEAQLAEQALRSGHVEVLINIWEND
                     SYSRYRPEKSVWNLYLLAQLPREMALPFWLRINEKKHLSAGEDYFLSIFGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTAANRWQRTDVWPALEHLLKQSPIEIYPA
                     RMPKAPDFWHPLMWSRPRLIINHQLITDDALEIIGEMLRFTQGGRFHSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSTR
                     AVSGLNILTLMNNDMALIQMHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRTDDDQLKAPE
                     ALARLKGLKKDTTQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTEGMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSAT"
BASE COUNT         2909 a         2893 c         2743 g         2653 t
ORIGIN      
        1 cttaaatcat tcatgtgaat gatttaagct ttctaaatcc gattaaaacc agggtggcaa
       61 attaatcgta gcgatttaac acgattcgac gtttgctatc gctatctgca gcaatcactt
      121 ccgtcgttac acctttaact ggcgaagaaa cctgatgacg ggtatcaaca ttatacgtta
      181 actgaccaca atgctgttga ggttgaccat taaccatagc catactgcat gtcgggggac
      241 tgatcctacc attaatcgtt aatgtggcag tttctgaaga gaacgcggga gaggccaatc
      301 cataggcaag aaaaataatt cctgacagcc aatacttatt cattgaacgt tatccctgta
      361 gtaaaggtta tgcctggata gaatgagtgc ataacaaact atagctgtac atccactaca
      421 cagccacgaa ggatgataat gaagcattgc ctgtacgatc aatcgacttt gtagagtttc
      481 ggacgaaggt ccgcagaata ttcgcagcat taaataagtg ttcattaatg cggcaaaaac
      541 aaatacaccg catcaatgta acatctctgc ggtgtatgaa gaataaccag aatggttaat
      601 acgcgttaga ccgcgcggaa ggaaatctcg cctggaatga cttcaccctg ccagtaaagc
      661 tgagctgcaa cgcggtcagc cagctggcgg tagatagcgg taaattcgct ctcaggacgg
      721 ctgataaccg ttggcgtgcc tttatcgaga tcctcacgta aggagatgtg taacggcatc
      781 tgccccagca gctgtgtgtg gtatttctcg gccagtttct ctgcaccacc ggtaccgaaa
      841 atcggttcat ggtgaccaca gttactgcaa atatgcacac tcatgttctc gacaataccg
      901 agcaccggaa cctcgacttt ctcgaacatc acaatgcctt ttttcgcatc gatcaacgcg
      961 atgtcctgcg gggtggtgac cacgactgca ccggttactg gaatgttttg tgccaatgtc
     1021 aattgaatgt caccggtacc tggcggcata tcaagcacca gatagtcgag atccggccac
     1081 aaggtttcct gcaacatttg cattaacgcc ttgctcgcca ttgggccacg ccacaccatc
     1141 gcattgtcgt ctgtcaccag ataaccaatt gaattagttg ccaggccatg agacataatc
     1201 ggtgccatat gggtaccgtc cggcgaagtt ggacgctgat tttctgcacc cagcatgtta
     1261 gggattgatg ggccgtagat atcggcgtcc agaatgccga ctttcgcccc ttccgccgcc
     1321 agtgccaacg ccaggtttac cgccgtggag gatttaccca cgccaccttt accggaactg
     1381 accgcgataa tgttcttcac gccgttaatt cccggctgat tcttcacgcg tttcagagtg
     1441 gctatgttat gcgacagttt ccagtcgata gctttcgctc cggtgatgcg cagcagttcg
     1501 gcgctacatt gctcttttaa ctcttcgaac gcgctatgcc agacgaatgg cataaccagt
     1561 tccacatgca gcgtgtcgtc catccaggcg acatggtgca acgctttcag cgttgtgagg
     1621 ttatgcttca gggttgggtg ctgaaaattg gccagtgtcc cggcgaccat tgcgcgcaag
     1681 gcttctggtg atttggcctg ggattgttcg ttcatcccga ctcctttttt gtatagataa
     1741 accatcagtt gatagtttac ctgaagaata tagagaagta cttacttaac attttcccat
     1801 ttggtactat ctaagccctt ttcactatta agaagtaatg cctactatga ctcaagtcgc
     1861 gaagaaaatt ctggtgacgt gcgcactgcc gtacgctaac ggctcaatcc acctcggcca
     1921 tatgctggag cacatccagg ctgatgtctg ggtccgttac cagcgaatgc gcggccacga
     1981 ggtcaacttc atctgcgccg acgatgccca cggtacacca atcatgttga aagctcagca
     2041 gcttggtatc actccggagc agatgattgg cgaaatgagt caggagcatc agactgattt
     2101 cgcaggcttt aacatcagct atgacaacta tcactcgacg cacagcgaag agaaccgcca
     2161 gttgtcagaa cttatctact ctcgcctgaa agaaaacggt tttattaaaa accgcaccat
     2221 ctctcagctg tacgatccgg aaaaaggcat gttcctgccg gaccgttttg taaaaggcac
     2281 ctgcccgaaa tgtaaatccc cggatcaata cggcgataac tgcgaagtct gcggcgcgac
     2341 ctacagcccg actgaactga tcgagccgaa atcggtggtt tctggcgcta ccccggtaat
     2401 gcgtgattct gaacacttct tctttgatct gccctctttc agcgaaatgc tgcaggcatg
     2461 gacccgcagc ggtgcgttgc aggagcaggt ggcaaataaa atgcaggagt ggtttgaatc
     2521 tggcctgcaa cagtgggata tctcccgcga cgccccttat ttcggttttg aaattccgaa
     2581 cgcgccgggc aaatatttct acgtctggct ggacgcaccg attggctaca tgggttcttt
     2641 caagaatctg tgtgacaagc gcggcgacag cgtaagcttc gatgaatact ggaagaaaga
     2701 ctccactgcc gagctgtacc acttcatcgg taaagatatt gtttacttcc acagcctgtt
     2761 ctggcctgcc atgttggaag gtagcaactt ccgcaagccg accaacctgt ttgttcacgg
     2821 ctatgtaacg gtgaacggcg cgaagatgtc caagtctcgc ggcaccttta ttaaagccag
     2881 cacctggctg aatcattttg acgctgacag cctgcgttac tactacactg cgaaactctc
     2941 ttcgcgcatt gatgatatcg atctcaacct cgaggatttc gttcagcgtg tgaatgctga
     3001 tatcgtcaac aaagtggtta atctggcgtc ccgtaacgcg ggctttatca acaaacgttt
     3061 tgacggcgtg ctggcaagcg aactggctga cccgcaactg tacaaaacct tcactgatgc
     3121 cgctgaagag attggcgaag cgtgggaaag ccgtgaattt ggtaaagcag tgcgcgaaat
     3181 catggcactg gctgacctgg ctaaccgcta tgtcgatgaa caggctccgt gggtggtggc
     3241 gaagcaggaa ggtcgcgatg ctgacctgca ggcgatttgc tctatgggca ttaacctgtt
     3301 ccgcgtactg atgacttacc tgaagccggt actgccgaaa ctgactgaac gagcagaagc
     3361 attcctgaat acggaactga cctgggatgg tatccagcaa ccgctgctgg gccataaagt
     3421 gaatccgttc aaagcactgt ataaccgtat cgacatgaag caggttgaag cactggtgga
     3481 agcgtctaaa gaagaagtga aagccactac cgcgccagtt actggcccac tggcagatga
     3541 tccgattcag gaaaccatca cctttgacga cttcgctaaa gttgacctgc gcgtggcgct
     3601 gattgaaaac gcagagtttg ttgaaggttc tgacaaactg ctgcgcctga cgctggatct
     3661 cggcggtgaa aaacgcaatg tcttctccgg cattcgttct gcttacccgg atccgcaggc
     3721 acttattggt cgtcacacca ttatggtggc taacctggca ccacgtaaaa tgcgcttcgg
     3781 tatctccgaa ggcatggtga tggctgctgg tcctggcggg aaagatatct tcctgctaag
     3841 cccggatgcc ggtgctaaac cgggtcatca ggtgaaataa tccccccttc aaggcgctgc
     3901 atcgacagcg ccttttcttt ataaattcct aaatttgttt tcttgcgatt ttgtctctct
     3961 ctaacccgca taaatactgg tagcatctgc attcaactgg ataaaattac agggatgcag
     4021 aatgagacac tttatctatc aggacgaaaa atcacataaa ttctgggcgg tggagcagca
     4081 gggaaacgaa ctgcacatca actggggtaa agtcggcacc aacggacaaa gccaggtaaa
     4141 aagttttgcg gatgcagcag cggcggaaaa agcgggactg aagctgattg cagagaaggt
     4201 gaagaagggg tatgtggagc aagcgaagga taattctttg caaccttccc aaacggtaac
     4261 ggactctctc aaggtagctg acttatccac cattattcag gaacaaccct ctttcgtagc
     4321 agaaacccgt gcggctgaca aaaatacaga tgctgtttta ccgtggctgg cgaaagatat
     4381 tgctgtcgtt tttccgcccg aagttgtaca caccacgtta agtcatcgcc gctttcccgg
     4441 agttcctgtt cagcaagcag acaaattgac ccaattacgt cgcttagcct gtagtgtgtc
     4501 gcaacgggat aataaaacag ccacatttga cttcagcgcc tgttctttag aatggcaaaa
     4561 caccgtcgcc caggcgatca gtcagatcga cggcctgaaa acaacacagc taccatcacc
     4621 cgtaatggct gtactcactg cacttgaaat gaaatgcaca agatataaag agcgtgagga
     4681 tgttatggat cagattattc aggaaggcgg tctggaatat gctactgatg taataataca
     4741 tcttcaacaa atatctattg aatgggatta tgtcaacaat aatattgttt tcctgtcgtc
     4801 tggcatttca cctgattact tgcagcaata ttccagcttt gaattacgcc tacgtaaaca
     4861 tttatcactg gcggaagagt ctctctggca aaaatgtgca caaaaactta ttgccgcaat
     4921 tccacatatt ccagaatggc ggcaaccatt aattgctttg ttattacccg aaaaaccaga
     4981 aattgcacat gaaatttccc agcgtctact ggggcaaaag aaattaccct cgcttgagtg
     5041 gctaaaaata gtggcgactg atgagcacat tcttgcctca ttagaaaaat atcacgagcc
     5101 atatgccatt tttgatgatt actattgtgg tgcgatatgg tcagccaccg tattacagga
     5161 gcaaggtgtt acagccctgc cccgatttgc tccctatgcc gcaagtgact actgcgccga
     5221 tgtgttgcgt catatcaatc atccgttcgc attaacactg cttatacgtg tagccgggca
     5281 tactaaacgc tgtcacgatc gaatgacgaa agcctgcgct gcgttcccac acgcagcact
     5341 ggcagcactg gcggaacttc tagtgcaaaa agaagagaat agttggcgca ttatgctaat
     5401 gacgatgctt atctcgcatc cgacactagc agaacaggtc attccctggc tttcgacacc
     5461 cgcagttgca gtgctgaaat catgtcagca acaactgaca cagccctcaa accatgccag
     5521 cgccgatcta ctgccggcca tagtagtctc cccaccatgg ctttcgaaaa agaaaaaatc
     5581 gccgattccg gtgctggatt tagcaccgct caacctcgaa tccatatgca caataaccga
     5641 cacagaagct aaagagtttc aaactcattg ggactgggaa ccgcataagc caagtgaagg
     5701 tgctaaaaat ttcctttact cgctggggta tcgccgctgg gattttgaca catataaata
     5761 tatcggcgct tctgactcgg caattgacgc atgggaacgt gaggacttcg ctacactcat
     5821 ccaaatgttt aaagcccacc atgcaccgta tcagggggag tggcatctca actcacttcc
     5881 ctttttacct atgcaaaaag cgattaagct atgggaattt ctcagcaaag agccgcatac
     5941 cgcgataaaa ccagtcatgc tttatttgcg actggcaggt atgagtggtt ttctacactc
     6001 gttttcacgc taccctcaag aaggttttgc ggttgctaat tattttgccg caacggaact
     6061 ggctcctgcc gtcgcccgcg ccttcaacaa actcaaaaca ttacgacaag acgccagtag
     6121 ctggctgctg aaatacccgg aacacgccat taccggcctg ctgcctgcag cgctcggcaa
     6181 agccagtgaa gcacaggaca acgcccgtgc tgccttgcgt atgcttaccg aaaacggtca
     6241 tcagccattg ctgcaagaaa tcgcccggcg ttataaccag ccggaagtaa ccgatgcggt
     6301 gaacgctcta cttgcgctcg atgccttaga taatcacccg acaaaaatcc ccactcttcc
     6361 gaccttttat cagccatcgc tctggacgcg cccgttatta aaagcaaacg cccaatcact
     6421 gcctgatagc gcactcctcc acctcggtga aatgctccgc ttccctcagg aagaggcact
     6481 gtatccggga ttattgcagg tgaaagatgc ctgtaccacc gactcactgg ctgaatttgc
     6541 ctgggatctg tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt
     6601 cactgcgtta ggcgttctcg gtaacgatga caccgcccgc aaactgacgc ctttgattcg
     6661 cgcctggcct ggtgaatccc agcataaacg cgccaccgtc gggttggata ttctcgctgc
     6721 tatcggtagt gatatcgccc tgatgcagct taacggcatc gcccagaaac tgaaattcaa
     6781 agcattacag gagcgggcaa aagaaaaaat tgccgacatt gccgagagcc gcgaactcac
     6841 ggtggcggag cttgaagatc ggttagcacc ggatctcggt ctggatgata atggttcgtt
     6901 gttgctggat ttcggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt
     6961 tgtgcgtgac gcttccggca gtcgtctgaa agacctgccc aaaccaaaca aaagcgatga
     7021 tgaattacag gcgaacaatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac
     7081 cgtcgccgcc cagcaggtag caaggctgga atccgccatg tgcctgcgtc gccgctggtc
     7141 accggaaaac tttcagctct tcctggttga gcatccgctg gtccgccact taacccgccg
     7201 tctgatttgg ggcgtttata gcgccgaaaa ccagctactg gcttgctttc gcgtagcgga
     7261 agataacagc tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc
     7321 tattggtatt cctcacgttc tggaaatatc accgacggat gctgccgcct tcgggcagct
     7381 ttttgccgac tacgaactgt taccaccgtt ccgccagctt gatcgtaaca gttacgcgct
     7441 gacagaagcc gagcgcaatg ccagcgaact gacacgctgg gcaggcagaa aatgcccgag
     7501 tggtcgggta atggggctgg caaataaagg ctggatgcgt ggtgaaccgc aggacggtgg
     7561 ctggatcggc tggatgatca aacctctggg ccgctggtcg ttaatcatgg aaatcgatga
     7621 aggttttgcg gtcggcatgt cgccagccga actcagtgcc gagcaactct taagcaagct
     7681 gtggctatgg gaaggcaaag cggaaagcta tggctggggg agtaattcaa cacaggaagc
     7741 gcagttctcc gtactcgatg ccatcaccgc cagcgagcta attaacgata ttgaagccct
     7801 gtttgaataa ggaaagagca tggacaagga attaccgtgg ctggcggata acgcccaact
     7861 ggaactgaaa tataaaaaag gcaaaacacc actcagccat cgcaattggc caggcgaacc
     7921 ggtacctgtt atcactgaaa acatcattca gacactgggt gatgaattgc tacaaaaagc
     7981 agagaagaaa aaaaacattg tctggcgtta tgaaaacttt tcactggagt ggcagtccgc
     8041 catcacgcag gccatcaact tgatcggcga acacaaaccc tcaatcacgg cccggacaat
     8101 ggcggcgcta gcctgtatcg cgcaaaatga tagccaacag ttgctcgacg aaatcgtcca
     8161 acacgagggg ctggaatatg caactgaggt ggtgattgca cgccagttta ttgtacggtg
     8221 ttatgagagt gatcctctgg tagtaacatt gcaatatcag aacgaggact atggctatgg
     8281 ttatcgctca gaaacctata acgaattcga tctccgattg cgtaagcatc tctctctggc
     8341 agaggaaagc tgctggcagc gttgcgccga caaactcatt gccgcactgc caggaataac
     8401 caaagttcgc cgccctttta ttgcgctgaa cctcccggaa aaacccgaaa tcgccaacga
     8461 gctggtaagc cttgaatgtt cgcaaactca ttttcgctca aaagagtggt taaaagttgt
     8521 tgctgatgac cctaaggcgg tgaaagaact cgcacgttac tggagccagg atatatttag
     8581 cgatcgagaa gccagctaca tgtcgcatga aaaccacttc ggctacgcgg cctgcgctgc
     8641 ccttttgcgc gaacaaggac tggcagccgt tccgcgcctc gcgatgtatg cacataaaga
     8701 agattgcggc agtctgctgg tacaaattaa ccatccgcaa gtcatccgca ccttgctact
     8761 ggtggctgat aaaaacaaac ccagcctgca acgtgtggct aaatacagta aaaacttccc
     8821 ccatgcgacg ctcgccgcac tggcagaact gctggcgtta aaagcccccc ctgcgcgccc
     8881 tggttatccc atcatcgaag accaaaagct gcctgcacag caaaaagcac gtgatgaata
     8941 ctggcatacg ctgttacaaa cgctgatggc atcgcagcca caactggcag aagaggtgat
     9001 gccgtggtta agtactcaag cccgggcagt gctgaatagt tatttatcgg caccgcccaa
     9061 aacggttatt gatagcaccg ataacagcca aatgcctgaa atcctcgttt caccaccgtg
     9121 gcgtagtaag aaaaaaatga cagctccacg tcttgatttg gcaccgctcg aattaacccc
     9181 gcaagtttac tggcaaccag gcgaacaaga gaggcttgcc tccactgagt ctgcccgtta
     9241 tttcagcacg gaatctcttg ctgaacgcat ggaacaaaaa agtggacgag ttgtattaca
     9301 ggaactgggt tttggggatg atgtatggct gtttctgaat tatatactcc ccggaaaact
     9361 ggatgctgca cgcaattcac tcattgttca gtggcattac tacccagggc gggttgaaga
     9421 gatcatgaat ggctggagct ccccggaagc tcaattagca gaacaggcgc ttcgcagcgg
     9481 tcacgtagaa gtgttaatta acatatggga aaatgacagc tactcacgtt atcgtccgga
     9541 aaagagtgtc tggaacctgt atttattggc acagttgccg cgtgagatgg ctttgccttt
     9601 ctggctacgt atcaatgaga aaaagcatct gtctgcgggt gaggactatt ttctcagtat
     9661 cttcggattg gatgctctac caggtctgct gttggctttt tcacatcgtc caaaagaaac
     9721 atttccgtta attttaaatt ttggcgcaac agaactggcc ctgcccgttg cccgcgtctg
     9781 gcgacgtttc gcggcgcagc gtgatctggc ccgccagtgg attttacaat ggccggaaca
     9841 tacggctact gcacttatcc ctcttgtctt caccaaaccc agcgataata gcgaagctgc
     9901 attacttgcc ctacgtttac tttacgaaca gggacatggt gaattgctgc aaaccgcggc
     9961 aaaccgctgg cagcgtacag atgtatggcc tgccctggag catttactta aacagagtcc
    10021 aatagagatt tacccggcac gcatgccaaa agcccctgat ttctggcatc cgctaatgtg
    10081 gtccaggccg cgcctaatca ttaatcatca acttattacc gatgacgctc tggaaattat
    10141 cggcgaaatg ctgcgcttta cccagggggg acgttttcat agcgggctgg aacaactgaa
    10201 aacgttctgc cagccgcaaa cgctggcagc ttttgcctgg gatctcttca ctgcgtggca
    10261 acaagctggt gcccccgcaa aagacaactg ggcatttctg gcgttaagtc tctttggtga
    10321 cgaaagcacg gcacgggatc taacgacaca gatcctcgcc tggccacaag aaggcaaatc
    10381 tacccgtgct gtcagtggcc tgaacatcct taccctgatg aataatgata tggcgctgat
    10441 acagatgcat catatatcgc aacgggcgaa atcccgcccc ttacgtgata acgcggcgga
    10501 atttcttcag gtggtcgcag aaaatcgcgg gctaagccag gaagagttag cggacagatt
    10561 agtcccaaca ctgggccttg atgatccgca ggcgttgagt tttgattttg gtccccggca
    10621 gtttaccgtt cgcttcgatg aaaaccttaa cccggttatc tttgatcagc aaaacgttcg
    10681 ccagaaaagc gttccccggt tgcgcaccga tgacgatcaa ctgaaagcgc ccgaggcact
    10741 ggcccgatta aaagggctaa aaaaagatac gactcaggtg agcaaaaacc tgctcccccg
    10801 tcttgaagct gccctgcgta ccacccgacg ctggtcgctg gcagattttc attctctgtt
    10861 tgttaatcat ccctttaccc gtctggttac ccagcgatta atatggggcg tgtatccggc
    10921 aaatgaaccg cgtcgtttac tcaacgcctt tcgtgtggcc gcagaggggg agttctgcaa
    10981 tgcgcaagat gagcccattg acctgcctgc ggatgctctg attggcattg cccacccgtt
    11041 agaaatgaca gaaggaatgc gcagtgaatt tgcacagctt tttgccgatt acgaaattat
    11101 gccgcctttt cgccagttgg cgcggcgcac ggtgctgctc acacctgacg agtcaaccag
    11161 taacagcctg actcgctggg aaggtaaatc cgctaccg
//
LOCUS       DAZGHF010000147        11084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGHF010000147 DAZGHF010000000
VERSION     DAZGHF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.148"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(457..1218)
                     /locus_tag="ACHK3A_004157"
     CDS             complement(457..1218)
                     /locus_tag="ACHK3A_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO8742168.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSTPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLCEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCIFY
                     LSWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            complement(1211..3307)
                     /gene="traD"
                     /locus_tag="ACHK3A_004158"
     CDS             complement(1211..3307)
                     /gene="traD"
                     /locus_tag="ACHK3A_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO8742169.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTEHSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     SEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTGSAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTTGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDALMPESIAKIAEAMRRNYHTGEGW
                     WNETPSVNMPEGEQNG"
     gene            complement(3304..4092)
                     /locus_tag="ACHK3A_004159"
     CDS             complement(3304..4092)
                     /locus_tag="ACHK3A_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742170.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGKRMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQAFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEALLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEALSASPEKALPRSWWRRLLQRGTRS"
     gene            complement(4089..4571)
                     /locus_tag="ACHK3A_004160"
     CDS             complement(4089..4571)
                     /locus_tag="ACHK3A_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742171.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRGGPAVMPWPDGIGISKVQERRAE
                     "
     gene            complement(4568..5161)
                     /locus_tag="ACHK3A_004161"
     CDS             complement(4568..5161)
                     /locus_tag="ACHK3A_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO8742172.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGRS"
     gene            complement(5158..5676)
                     /locus_tag="ACHK3A_004162"
     CDS             complement(5158..5676)
                     /locus_tag="ACHK3A_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO8742173.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMVVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            complement(5673..6353)
                     /locus_tag="ACHK3A_004163"
     CDS             complement(5673..6353)
                     /locus_tag="ACHK3A_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO8742174.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRDFRQPDVARPWPWTINVAGKGYRYATRLEAWQAL
                     QVFLKHYPAKRIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRRHPVS
                     WLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHVAATAMLTPSPRYIW
                     TEPQEEQK"
     gene            complement(6332..7051)
                     /locus_tag="ACHK3A_004164"
     CDS             complement(6332..7051)
                     /locus_tag="ACHK3A_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO8742175.1"
                     /translation="MKRKILYLLALCPVLAQSAGQTNMSSEAQRTTGTVVTQSTTSGL
                     QQQAGQWGVSEEDYRRYQSLMSGPRGIQSPGLDPLTVLGIEARTPAERRVWAEKWVRA
                     EYERTEKELAFQQEVNAAWKRLYPSILPVSMGKTGALTGDTGGRLALFVKAKECGTCD
                     ARLASVLATGRQVDIYLVDSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRF
                     GEGKMPVVLQQGADGWRVAAF"
     gene            complement(7055..7804)
                     /locus_tag="ACHK3A_004165"
     CDS             complement(7055..7804)
                     /locus_tag="ACHK3A_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742176.1"
                     /translation="MSEKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYG
                     VLMLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVL
                     TASWKEQLENLPEMLEQIESAREKLSLRLDTLSGLVEKMQKVPESPSQPDSRKAGMKK
                     TEEKKPAPVTSSRKSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLGE
                     GESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
     gene            complement(7801..8400)
                     /locus_tag="ACHK3A_004166"
     CDS             complement(7801..8400)
                     /locus_tag="ACHK3A_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFO8742177.1"
                     /translation="MKKRFILSGLTVCLLMAGCQSTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGREPGVKATGGFLKK"
     gene            complement(8586..9578)
                     /locus_tag="ACHK3A_004167"
     CDS             complement(8586..9578)
                     /locus_tag="ACHK3A_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO8742178.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYPYSMNWPDCLDDPAFGRELYGEGRPPRV
                     IDVGLLDDEGIRRYKQMAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFN
                     YMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRQEGIEKGRLEGIEK
                     GRQEGIEKGRQEEREKALQENYASARRMQQMGISSEKIKQILQFSDDDLRNALR"
     gene            complement(9627..10187)
                     /locus_tag="ACHK3A_004168"
     CDS             complement(9627..10187)
                     /locus_tag="ACHK3A_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8742179.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKEHTEWHRVVIYGKLAEIAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYST
                     EVVVSVNGTLQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGM
                     PPMDFDDDIPFMGRGYGVERRIIHVI"
     gene            complement(10203..10406)
                     /locus_tag="ACHK3A_004169"
     CDS             complement(10203..10406)
                     /locus_tag="ACHK3A_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742180.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(10497..10964)
                     /locus_tag="ACHK3A_004170"
     CDS             complement(10497..10964)
                     /locus_tag="ACHK3A_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO8742181.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
BASE COUNT         2554 a         3388 c         2638 g         2504 t
ORIGIN      
        1 aataaactat gttctggggg ctgtcagcat agccagtttc tgcatctcgc tgttccagat
       61 tctgttcaaa taaatttatt ccagcctgaa tgatttattc ctgtttatta aaacacggca
      121 tcactactgt ttcccgttaa tgacacctca ttaacaattt aaagaggaaa cacatcagca
      181 tggaatttct ccgtactgcc cttccgttct gggccctgtt ccttctgacc tgggtttgtg
      241 ttgtgtattt tttcatttat gaagaacctc gttgcccccc gtggatctat gacgtcatca
      301 gggccatcac tctgttcatt attttcgtca acattgttgt cattccggcg ctgtcgttac
      361 tctgacactg acgcgcactg accgtaaggg cgtggcttgt ctgccgacac cctgccacaa
      421 aacaagtttt gcatcagggt gtccggcctc tgtggattat aaatatttct tgaaggatgc
      481 catggtcacg gtgatggcca cccccagcag catggccgcc ggccatatca gcaggttcgg
      541 atacaccgac gtcggccagg acaggtaaaa aatgcaggga atatatatcg ccggtttaat
      601 agactttttt gcatggtgat aaacaaagct ggactcatac gcagcaccat accggcgcag
      661 atcacgacgc cccagtccct ccacaaccgc gatcagtatc accatcacaa acagcggaac
      721 agacaacacc aggatcgtca cccgtatcag tgtgaccacc gtgacatata ctgtggccat
      781 caggtactca cacagataat ggctcagcca gcgccccaca tcctcactgt actgtgacac
      841 actgttaccc accacggcac cggcattttg ctgcttcagc cacgacagaa aacctgtttc
      901 cacaaacagc cactgatacg ccgttgctat ccattctgtc accgttaccg atggtgtcga
      961 cagcagcaga ctgcgggtga attccgaaga cagccacccc agttctgtct tcatcactgc
     1021 ctcactgtgt gctgccccct gctccggcca gaaaaaagca atcccgatat actcaaccag
     1081 caggctcagc agcagggagg aaaacaggat cccgaccagc atccacggcc agccccacaa
     1141 cagattccag agcagtccgt gctgacgtgg cggagagggt tgtgctgctc tgttcacctc
     1201 acgacggggt tcagccattc tgctctcctt ccggcatgtt cacggaggga gtctcattcc
     1261 accatccctc tccggtgtgg taattacgcc gcatggcctc tgcaattttt gcaatactct
     1321 ccggcatcag cgcgtcatct gcatcagttg caggcagggg catacggatt ttccacaact
     1381 gtcccccttc cagcagagca aaggcctgtc ctttcggcag ggtcacgata tctgccggct
     1441 ccagcaaagg caccttcaca gtaccaacac gatcctgggt actggatgtg aagtcctggc
     1501 cggtcgtgac atctgcggta tcctgatgac cggaaaccag cgttttggta taaatttcca
     1561 cctgggacaa ctgggtcgtc agtaactcgg ccgtccggtt ctccctgacc cgcagcataa
     1621 tcatgttatt aaagttaccc tgcacctggg ccgttttggc agcactaccg gtacgggcct
     1681 caatatcaga agaggtctgc gtgtaagccg tcacctgcat accggcaccg cctcccttgt
     1741 tgattaacgg aatgaactca tcgcccatca gttcattaaa ctcatcacaa tgcaggttaa
     1801 ccggattttt ggtaccattc cctcccggaa gaccggcatt cattccgtgt ttgtagatat
     1861 gcccggcgac actcaccaga tccgcaaaca tggagttacc cacggcactg gccacctcac
     1921 tgtcactcag cgcatcaaga ccaatgtaca ccaccgcctt tttacgtatc acctgctccc
     1981 agtcaaacac gggacgggta tcgctcatat ccaggtagtc aggtgccagc agttctgccg
     2041 tctttcctgt ggtcagtttt tccagcaacg gcaacaggga tgccacaatt ttgtcaaaat
     2101 aggtacgatc ataacgtacc gcgcttcgca gaccatccag tatcggatca tagagtttct
     2161 ttccggcttc actgctgaga gccacctcaa tggcccagat acgcacagca tccggctggt
     2221 tctgcatggt ccggggaaca tccgcttccc ccagtacatt catgttattc agaatggcgt
     2281 tttccagttc cggcatctgt tcgctgatca ccttaccagc ataacggata taaaggtcgg
     2341 caatattatt cacataacgc ataatcagcg tgtaatctgg gcgctccccc agtgccacca
     2401 gtgcacgggc aacaatgttg acaaaccgcc atgcaaactc acggaaggcc gcactgttac
     2461 cttcaccgga taactgaccg gccacacggg aagccacctc cgacacacga ccaaaacgcc
     2521 ccacggcgtt ataacgcgca gagatttccg gccagcccag atgaaagata taaagctcat
     2581 cgcccctgcc ggcacggtgt gcttcagccc agacacgcag caacagatcc gcatcccctt
     2641 tgggatcaaa gacaatggtg acatctcccc gacgaatatc ctgtgtgacc agcaactccg
     2701 ccagccgggt tttcccgaca cgggtggtgc catagacaat ggtgtgcccc acacgttcac
     2761 ccagtgccag cgtcacatcc tgctcatccg gctcaatacc atgcagggca ggatttcctc
     2821 ccaccggcgg caggggacgg acgggattca gcggcgagtc acgacgaaga agctgcccaa
     2881 tccagggaag actgtgctcc gttttcattt caagccagcg cgctgtccgg tacagactgc
     2941 ccggctgtag atagtgtgca acctccggtc tcagcgtgtc ctgaaggcgc tgggtgtgct
     3001 tctgtgtcca gcggaagccg cgtcccagaa acagccgctg acggctgacc ggaatttgcc
     3061 gggaactcat cacataacgg ggcagccggc gcagattgcg acggtaacgg atgatcttca
     3121 ttccctgccg gaaacggacc actgccagaa cgccaaagcc ggcggctgtg acatagctga
     3181 ccgaaggcgc gagcgccacc gcccagggcg ctgtcacaca gacaccggcc gccgttgctg
     3241 ccaccacggc ggtattcagt tccacagccg gacgcagcag agcctcaatc acataacggt
     3301 tactcatgac cgggtacctc tctgtaaaag acgccgccac catgagcgcg gcaacgcttt
     3361 ctccggtgat gctgacagtg cctccagtct gcgctgtaac accatcacct cccgccatgc
     3421 gtctgccgca ttcagcagta atgccttata caccagtggc ccgtcatgtc cgccggcatg
     3481 cacatcatcc actgtcaaca gcgcctctgt tctgaaacgg tccactttcc agcaggccag
     3541 gtcttcgcgg gaaacacact gccgccggat ctgcagacga agatacacat catgccggga
     3601 caacggttct gccaggttgt gaatttcccg acaggcagac cggaacgcct ggtctgtgtg
     3661 ataaatactc tgggccacat tgtgaagctg tgtgcgcccc tccccctgca ggtggaacag
     3721 aaagcgggcc agacagctgc ggtgattacc gtcagcactg acataccagt gaatgccatc
     3781 cgtgctgaca aaacccatcc cggcatgctg ttgctctgcc tgcgtgtaat atcccggatt
     3841 cttttcaagt aacgggatat tgatgtccat gcgtttacca cgctccagca gctccagcca
     3901 gctcattccg gcatactgtg gatggtcagt ccccacaatg cggtacacat tgatactgcc
     3961 ctgtcccgtc cagtagtgtt ccgtaataaa atccgacatt gccgcaaact ctgcggtatc
     4021 ccatgcaacc agttgttcat gtgcccaggc cggagcattc gcctgtatcc gctctgtaaa
     4081 tgcccccttc attcagctct tctctcctgc acttttgaga taccgatacc gtcaggccac
     4141 ggcataacag ccgggccccc cctgccttcc ccggatacgg ctgcagaacc acaaccacga
     4201 aacagaatga ctgacgagac gccttccata aacaaagcgc ccagcaacag aaccactgcc
     4261 tgcattttca gttcacgaat atcctcagca acagcctccc cgaaagaaat cgctgttcgc
     4321 ggacattcct gctcatcgac atacatacct gagacggata tgacggagga acatttttgt
     4381 accatcacat gcccttcagg cgcatcacgc cagcgggatg actccgtcgc ataacgctgt
     4441 gtcatgcctg cctcaccacc actcagattg ttccagacat acagcattac cagacatcct
     4501 gccagcattg acggccagac aatcagaagc cggagcagca gcataacaaa ccggtacagt
     4561 gtcataatca tgatcttcct ttattactca gtaaggtgcc attcagcagt tgcaacagac
     4621 gttgtccact gatcagctca atatccggcc acagtgtcag ccgtgcccgg ctgatgttcc
     4681 ccgtacgccc cgtatggata aaaaagcccc tgcatccctt ccggcgcagc agttcgccaa
     4741 aagcatcaat atgtgccgga cgaatggttc ttccgtaacg tttagcctgt atcagccagc
     4801 gttcaccgtt aatcaccacc tgaccatcaa gaccaccatc gccgctgtat gaggggttac
     4861 ggataaccgg catcccctgt tgctcaaaag ccagcagcaa cagttcctca aacacatacg
     4921 gactgatttt gcgcagatat ttcagccgcc gggcatcacc ctgcaattcg ttcagccgct
     4981 tcattacccg tgaggccgtg gcacgataac gctgatgccg gcgtgttctc acacacgctt
     5041 tttttcgccg gctgcgccac agtgccggcc ccaggacaac agccagcagg ctgagaacca
     5101 ggcaggctgt cagtaccgga gaagcagaga gcgcagcaga agtggcagac agcggcatca
     5161 tggtgcaggt acctgtgaca ggccgttgtc cgttatcagc accgggtaat gacttatctg
     5221 taacctgcgg gccagtacag aaccggatgc cggaaccatc accacccccg gtgccagctc
     5281 acgcagtacg cgcagactgc tctcatccgc cacattcact accattcccg ccgccttccg
     5341 ctccctgagt actgctgcat tcatctgcag ccatgtgcgg gaatgggcat catcgcccac
     5401 aagaaaaagt gcaccgatgc ccggtaactg aagccggcgc gccacaacct ctccgggtga
     5461 catttcaggc gtactgaccg gtaacattgc cgcctctccc tgcggaaaag agggtgatgg
     5521 cacaactgaa gcgggaaccg gtggctgccg gtttatcgcc tcaaaaaagg ggctggcatc
     5581 cctgccgccc aggtctgcaa tcactttcag ttctgcgcca gctgttgtac agaacagcag
     5641 cagactcagt acataaaact gtctttctcg tttcatttct gctcctcctg tggttcagtc
     5701 cagatataac ggggggatgg tgtcagcatg gcggtcgctg ccacatgcac atcagacgga
     5761 ttcaggaaat tatcccggtg gagccgggca agatgccgtc tgaccacggc ggtataacgt
     5821 gcggccggcg caccacctgc cgggtggtga taacacccgg ccacctgcag ccagctgacc
     5881 ggatggcgac gccggcattc ctgcagaatg accgctgccg tattcagact ggtgtaagga
     5941 tcaaatgccg cccatgtgga cgggaaacga tgtccattcc agcccagatt gacctgtgct
     6001 atcccgacat caatacgttt tgccggataa tgtttcagga acacctgcaa tgcctgccag
     6061 gcctccagac gtgtggcata acggtaccct tttcctgcca catttattgt ccagggccag
     6121 ggtcgggcca cgtccggctg acgaaaatca cgacgccgtt gtttcagtac tccggacatc
     6181 gcttccggag acatggacgt ttccgtcagg gaaacggcat acaaaagctc tgcgggaata
     6241 ccatgggcac gggccacccg cacatacccg tccggtacgt tctgaacggc agcacgacag
     6301 acaccactgc aggccaccat cagcagaacc gtcagaatgc cgccacgcgc catccgtcag
     6361 ccccctgctg cagtaccacc ggcatttttc cttcaccaaa acgcagccag cgtccggcat
     6421 catgattcag ggtgatatga cggctgcgaa ctttctccac aggtatgctg tgcgcatgcg
     6481 cccactgacg cagaatctca tcctttccct gactgtcgac aaggtagata tcaacctgcc
     6541 gtcccgtggc cagtacggag gcaagacggg catcacaggt gccacattcc ttcgccttca
     6601 caaacagcgc cagccgtccc cccgtatctc cggtaagtgc tcctgttttt cccatactga
     6661 caggcaagat actgggatac aggcgcttcc aggccgcatt cacctcctgc tgaaacgcca
     6721 gctccttctc tgttcgttca tattcagccc tgacccactt ttcggcccag acacgtcgct
     6781 ctgccggcgt tctggcctct ataccgagta ccgtcagcgg atcaagtccg ggagactgta
     6841 tcccccgggg gccggacatc agtgactgat aacgccggta atcctcctca ctcacccccc
     6901 actgcccggc ctgctgctga agtccggatg tggtactctg cgtcaccacc gtaccggtcg
     6961 tccgttgcgc ctctgacgac atattcgtct gcccggcgga ctgagccaga acggggcaca
     7021 gtgccagcag atacagtatt tttcgcttca tcatctactc caccctgagc accgtctgac
     7081 gaccattcac ccggaacgtt gcttcaccat ggccggcata cacaagcgtc cacccggcca
     7141 cactctctcc ttcacccagc agagccacct gagagaggct ggtatacccc cggggagcca
     7201 cggctgcacg ggtactgcca ccgcgcagct ctgtcccggt cagcacaaaa ggagcctgcc
     7261 gaactgtatg aactgcgcgg gatttacggg aggacgtcac cggagcgggc tttttctcct
     7321 ccgttttttt catccccgct ttcctgctgt ccggctgtga cggtgactcc ggtactttct
     7381 gcattttctc caccagaccg ctgagtgtat ccagtctcag ggaaagcttt tcccgggcag
     7441 actcaatctg ctccagcatc tccggcagat tctccagttg ttccttccag gatgctgtca
     7501 gcacgggctg ctgctccagg actgccaggc gcttttccat atccttcagt tgcttctccg
     7561 tctctgtcag tttcattctc aggggcgcga gttctgacgc agaaagcatt tctcttctct
     7621 ggccttccag ggtcgtgatc cggctgtcaa gaccggtcat aagcatcagt acgccatata
     7681 ccactgcccc catgaccagc cccacagtga cagacagtat caacacgccg gggaaacgac
     7741 tttttcttcg ctccggcgac ataccagacg caccctccgg tatctgcggc tgtttctcac
     7801 tcattttttc agaaaccctc cggttgcctt cacacccggt tcacgaccca caaaaggaac
     7861 ggtgaccgga acaggagcca gctggctgac cggtaactgg taaccgtcac gcagactgtg
     7921 gcagaccacc ctctgcacat catccacttc cagttgccat gccggccccg ccattaactg
     7981 cagggcagta cgcagtctca gtggtcccgg ctgatactgc accgccggca gaggctgacg
     8041 gtacagcacg ccgttggccg gtccggtggc gcagagcgtg taaccggact gtttcagggc
     8101 ataacgcatg gcatcggcca ccgtgggttt cagggaggag ggaatgcgaa tgtcgatcac
     8161 ctgagaaagt ggatcacgct gtacagcctg cggctctgtg ctgaccagca ggtaacggtc
     8221 atagcgcacc acctccggtg cctgagaata cacatccggg ctggcaggct gtacattacg
     8281 gtttactgtg accgacggta ccggcatggc cgcatcccgt tgctgtagtt tctgagtgct
     8341 ctgacatccc gccatcagga ggcagactgt cagtccggac aggataaagc gttttttcat
     8401 cgggtattta cctctgcagt gataaatcag aggtcaatgt gcagaagaag acggggctgg
     8461 ctcaatgaac aactcattac ccgctggcga aaaaaaacgc ctgcagacat gcaggcgctt
     8521 tgttgacagg aaagaaagca acataacaaa tatgtcacaa tcctgcggtt aacataaatt
     8581 accgattacc ggagagcatt acgcaaatca tcatccgaga actgcagtat ttgtttgatt
     8641 ttctctgacg atatacccat ctgctgcatg cgacgggccg atgcgtaatt ctcctgaaga
     8701 gccttttctc tctcctcctg gcggcccttc tctatacctt cctggcggcc cttctcgata
     8761 ccttccaggc ggcccttctc aataccttcc tggcggccct tctcgatacc agccagttcc
     8821 agtcgttcag cgatagtcat tagatcatcc tcatgtcttg gcagacgttc tgccatcgcc
     8881 cgtataaatt caggtgaagc tgaatcacat tctttcacca tatagttaaa caatgtcacc
     8941 acctgagtgt gtgtttcctg tatctgaatc aactgtgaca aaaagtcaag atgaaccatc
     9001 agatccactt tcttctgccg gagcttcatc ataagcatca gcgcagccat ctgcttatag
     9061 cgacggatac cctcatcatc aagaagaccg acatcaatca ccctgggtgg cctgccttct
     9121 ccataaagct cccgaccaaa cgccggatca tccagacagt caggccagtt catgctgtat
     9181 ggataaggac tgggtacacc atggtaaaaa agcaccggta tcacaacggg agcacacgca
     9241 tgtgctttca ggtggcgatg tatcaccgcc agaacatagc gcatcattcg cagggccata
     9301 tacttatccg ccttgctctg gtgctctgtc agcgtataaa tgtatccatc cctgccatct
     9361 gtggttttca tactccacag aacatcacag gcaaactggc gaagatccgg atccacaaac
     9421 gtggtcggct ccagttgcag ggtgttcata tcgcaccggt tcagttgttc cgggggcaac
     9481 gcgcctttca ggaaatccct cgcaacagaa ggacattcca tcatcgctct gaacgccgca
     9541 tcatgtggcg tgggggtagt gggtgtgttt tcattcacaa ccgcctcctt gaattaaaag
     9601 tggggcggta agccccgtgg ttaacatcag atgacatgga taatccgtcg ttcaacgcca
     9661 tacccacgcc ccataaaggg gatatcatcg tcaaagtcca tcggcggcat tcccccgctt
     9721 cctgaggaag cctgaccgtt ttttcccgtt gattgttgtg cattttgtgg tggttgtccc
     9781 catcctccct gagcaggagc agatgaaaca tttccaccac cctgctgctg acgcggagac
     9841 cccagcatct gcagggtacc attgacactc acaacaactt ccgtggaata acgatcaaca
     9901 ccattctgct gatcgaacca cttccgggtt cttaactgac cttcaatata cacctgtgaa
     9961 cctttacgaa ggtattcacc ggcaatctca gccagttttc cgtaaataac aacacggtgc
    10021 cattcagtgt gttccttcat ttcaccgctc tgtttatcac gccaggtatc agtggtggcc
    10081 agcgtcagat ttgccactgc acctccgccg ttggggaaat aacggatttc cggatcctgt
    10141 cccagatgac caatcagtat gactttattc actccacgcg atgccataac aactccttat
    10201 cctcacacac attcacgctg agtccagatg ccgttttcca gagcccggac tgcctgctca
    10261 taatttctga aatactcttc ggattcccgg gacacaggcc cttcgtcgca ctgagtacca
    10321 atataataac cacgagcact gctgaggatc tgcagaggta aggtatagcc cgctttggcg
    10381 gccagaattc cgcttgtcgg tttcataaca tgtttcctta aagttaaacg ataagaaaac
    10441 agacctccct gatggttggt ctgttttcat cagggaggtt gagttgaaaa atacgatcag
    10501 aaagaatttt ctgcgtattc ccgcggggta ttgccattct gtggcggtat ggattccgat
    10561 ttttcagtct tataaaccat gtcctggcct attttgatcc actccacctt gatcaggcgg
    10621 gctttcagac tgacgcgttg ctcaccggca tgttcacccc ggttaagggt aaaaatgtca
    10681 gtagaggggt tgctcatggt aaaaccaatc agcaccttgc gatcttcatc caccgctttc
    10741 tggcagcggg caatcaggga agttgcctct ttaccggcaa ccgtcacatc aaaacgcaca
    10801 taggacgggc tgtccgtcgg accactcagt gcattcacaa cacaactgag aaatgtcccg
    10861 ttctgatgat taacctgacg aatgttgctg agatacccca ggcctttcag tgtcagattg
    10921 aagtactcag ttttagcgtt tgcagaagaa gtatttgcag tcataatgat atctccattc
    10981 tcagtatcgg aaagacagag aacatcatca tccctgacgg gttgtgatat tcccgtcagg
    11041 gagcaggaac agaactattc cggctgactc agtaacagtt aaag
//
LOCUS       DAZGHF010000148        11075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGHF010000148 DAZGHF010000000
VERSION     DAZGHF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.149"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            364..744
                     /gene="atpI"
                     /locus_tag="ACHK3A_004171"
     CDS             364..744
                     /gene="atpI"
                     /locus_tag="ACHK3A_004171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO8742182.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            753..1568
                     /gene="atpB"
                     /locus_tag="ACHK3A_004172"
     CDS             753..1568
                     /gene="atpB"
                     /locus_tag="ACHK3A_004172"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO8742183.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1615..1854
                     /gene="atpE"
                     /locus_tag="ACHK3A_004173"
     CDS             1615..1854
                     /gene="atpE"
                     /locus_tag="ACHK3A_004173"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO8742184.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            1916..2386
                     /gene="atpF"
                     /locus_tag="ACHK3A_004174"
     CDS             1916..2386
                     /gene="atpF"
                     /locus_tag="ACHK3A_004174"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO8742185.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            2401..2934
                     /gene="atpH"
                     /locus_tag="ACHK3A_004175"
     CDS             2401..2934
                     /gene="atpH"
                     /locus_tag="ACHK3A_004175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO8742186.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            2947..4488
                     /gene="atpA"
                     /locus_tag="ACHK3A_004176"
     CDS             2947..4488
                     /gene="atpA"
                     /locus_tag="ACHK3A_004176"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO8742187.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            4539..5402
                     /gene="atpG"
                     /locus_tag="ACHK3A_004177"
     CDS             4539..5402
                     /gene="atpG"
                     /locus_tag="ACHK3A_004177"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO8742188.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            5429..6811
                     /gene="atpD"
                     /locus_tag="ACHK3A_004178"
     CDS             5429..6811
                     /gene="atpD"
                     /locus_tag="ACHK3A_004178"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO8742189.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            6832..7251
                     /locus_tag="ACHK3A_004179"
     CDS             6832..7251
                     /locus_tag="ACHK3A_004179"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO8742190.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            7600..8970
                     /gene="glmU"
                     /locus_tag="ACHK3A_004180"
     CDS             7600..8970
                     /gene="glmU"
                     /locus_tag="ACHK3A_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO8742191.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQSQKEGWR
                     RPVKKK"
     gene            9132..10961
                     /gene="glmS"
                     /locus_tag="ACHK3A_004181"
     CDS             9132..10961
                     /gene="glmS"
                     /locus_tag="ACHK3A_004181"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO8742192.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         2668 a         2737 c         3013 g         2657 t
ORIGIN      
        1 tcatttatta aaacagtatc tgtttttaga ctgaaatatc ataaacttgc aaaggcatca
       61 tttgccaagt aaataaatat gctgtgcgcg aacatgctca atatgtgatc tgaagcacgc
      121 tttatcacca gtgtttacgc gttatttaca gtttttcatg atcgaacagg gtttgcagaa
      181 aagtcgcaat tgtatgcact ggaaaaatat ttaaacattt attcaccttt tggctactta
      241 ttgtttgaaa tcacgggggc gcaccgtata atttgaccgc tttttgatgc ttgactctaa
      301 gccttaaaga aagttttata cgacacgcgg catacctcga agggagcagg agtgaaaaac
      361 gtgatgtctg tgtcgctcgt gagtcgaaac gttgctcgga agcttctgct cgttcagtta
      421 ctggtggtga tagcaagtgg attgctgttc agcctcaaag accccttctg gggcgtctct
      481 gcaataagcg ggggcctggc agtttttctg cctaacgttt tgtttatgat atttgcctgg
      541 cgtcaccagg cgcatacacc agcgaaaggc cgggtggcct ggacattcgc atttggcgaa
      601 gctttcaaag ttctggcgat gttggtgtta ctggtggtgg cgttggcggt tttaaaggcg
      661 gtattcttgc cgctgatcgt tacgtgggtt ttggtgctgg tggttcagat actggcaccg
      721 gctgtaatta acaacaaagg gtaaaaggca tcatggcttc agaaaatatg acgccgcagg
      781 attacatagg acaccacctg aacaaccttc agctggacct gcgtacattc tcgctggtgg
      841 atccacataa ccccccagcc accttctgga caatcaatat tgactccatg ttcttctcgg
      901 tggtgctggg tctgttgttc ctggttttat tccgtagcgt agccaaaaag gcgaccagcg
      961 gtgtgccagg taagtttcag accgcgattg agctggtgat cggctttgtt aatggtagcg
     1021 tgaaagacat gtaccatggc aaaagcaagc tgattgctcc gctggccctg acgatcttcg
     1081 tctgggtatt cctgatgaac ttgatggatt tactgcctat cgacctgctg ccgtacattg
     1141 ctgaacatgt actgggtctg cctgcactgc gtgtggttcc gtctgcggac gtgaacgtaa
     1201 cgctgtctat ggcactgggt gtatttatcc tgattctgtt ctacagcatc aaaatgaaag
     1261 gcatcggcgg cttcacgaaa gagttgacgc tgcagccgtt caatcactgg gcgttcattc
     1321 ctgtcaactt aatccttgaa ggggtaagcc tgctgtccaa accagtttca ctcggtttgc
     1381 gactgttcgg taacatgtat gccggtgagc tgattttcat tctgattgct ggtctgttgc
     1441 cgtggtggtc acagtggatt ctgaatgtgc cgtgggccat tttccacatc ctgatcatta
     1501 cgctgcaagc cttcatcttc atggttctga cgatcgtcta tctgtcgatg gcgtctgaag
     1561 aacattaatt taccaacact actacgtttt aactgaaaca aactggagac tgtcatggaa
     1621 aacctgaata tggatctgct gtacatggct gccgctgtga tgatgggtct ggcggcaatc
     1681 ggtgctgcga tcggtatcgg catcctcggg ggtaaattcc tggaaggcgc agcgcgtcaa
     1741 cctgatctga ttcctctgct gcgtactcag ttctttatcg ttatgggtct ggtggatgct
     1801 atcccgatga tcgctgtagg tctgggtctg tacgtgatgt tcgctgtcgc gtagtaagcg
     1861 ttgcttttat ttaaagagca atatcagaac gttaactaaa tagaggcatt gtgctgtgaa
     1921 tcttaacgca acaatcctcg gccaggccat cgcgtttgtc ctgttcgttc tgttctgcat
     1981 gaagtacgta tggccgccat taatggcagc catcgaaaaa cgtcaaaaag aaattgctga
     2041 cggccttgct tccgcagaac gagcacataa ggaccttgac cttgcaaagg ccagcgcgac
     2101 cgaccagctg aaaaaagcga aagcggaagc ccaggtaatc atcgagcagg ctaacaaacg
     2161 ccgctcgcag attctggacg aagcgaaagc tgaggcagaa caggaacgta ctaaaatcgt
     2221 ggcccaggcg caggcggaaa ttgaagccga gcgtaaacgt gcccgtgaag agctgcgtaa
     2281 gcaagttgct atcctggctg ttgctggcgc cgagaagatc atcgaacgtt ccgtggatga
     2341 agctgctaac agcgacatcg tggataaact tgtcgctgaa ctgtaaggag ggaggggctg
     2401 atgtctgaat ttattacggt agctcgcccc tacgccaaag cagcttttga ctttgccgtc
     2461 gaacaccaaa gtgtagaacg ctggcaggac atgctggcgt ttgccgccga ggtaaccaaa
     2521 aacgaacaaa tggcagagct tctctctggc gcgcttgcgc cagaaacgct cgccgagtcg
     2581 tttatcgcag tttgtggtga gcaactggac gaaaacggtc agaacctgat tcgggttatg
     2641 gctgaaaatg gtcgtcttaa cgcgctcccg gatgttctgg agcagtttat tcacctgcgt
     2701 gccgtgagtg aggctaccgc tgaggtagac gtcatttccg ctgccgcact gagtgaacaa
     2761 cagctcgcga aaatttctgc tgcgatggaa aaacgtctgt cacgcaaagt taagctgaat
     2821 tgcaaaatcg ataagtctgt aatggcaggc gttatcatcc gagcgggtga tatggtcatt
     2881 gatggcagcg tacgcggtcg tcttgagcgc cttgcagacg tcttgcagtc ttaaggggac
     2941 tggagcatgc aactgaattc caccgaaatc agcgaactga tcaagcagcg cattgctcag
     3001 ttcaatgttg tgagtgaagc tcacaacgaa ggtactattg tttctgtaag tgacggtgtt
     3061 atccgcattc acggcctggc cgattgtatg cagggtgaaa tgatctccct gccgggtaac
     3121 cgttacgcta tcgcactgaa cctcgagcgc gactctgtag gtgcggttgt tatgggtccg
     3181 tacgctgacc ttgccgaagg catgaaagtt aagtgtactg gccgtatcct ggaagttccg
     3241 gttggccgtg gcctgctggg ccgtgtggtt aacactctgg gtgcaccaat cgacggtaaa
     3301 ggtccgctgg atcacgacgg cttctctgct gtagaagcaa tcgctccggg cgttatcgaa
     3361 cgtcagtccg tagatcagcc ggtacagacc ggttataaag ccgttgactc catgatccca
     3421 atcggtcgtg gtcagcgtga attgatcatc ggtgaccgtc agaccggtaa aaccgcactg
     3481 gctatcgatg ccatcatcaa ccagcgcgat tccggtatca aatgtatcta tgtcgctatc
     3541 ggccagaaag cgtccaccat ttctaacgtg gtacgtaaac tggaagagca cggcgcactg
     3601 gctaacacca tcgttgtggt agcaaccgcg tctgaatccg ctgcactgca atacctggca
     3661 ccgtacgctg gttgtgcaat gggcgaatac ttccgtgacc gcggtgaaga tgcgctgatc
     3721 atttacgatg acctgtctaa acaggctgtt gcttaccgtc agatctccct gctgctccgt
     3781 cgtccgccag gacgtgaagc attcccgggc gacgtattct acctccactc tcgtctgctg
     3841 gagcgtgctg cgcgtgttaa cgccgaatac gttgaagcct tcaccaaagg tgaagtgaaa
     3901 gggaaaaccg gttctctgac tgcgctgccg attatcgaaa ctcaggcggg tgacgtttct
     3961 gcgttcgttc caaccaacgt aatctccatt accgatggtc agatcttcct ggaaaccaac
     4021 ctgttcaacg ccggtattcg tcctgcggtt aacccgggta tttccgtatc ccgtgttggt
     4081 ggtgcagcac agaccaagat catgaaaaaa ctgtccggtg gtatccgtac cgctctggca
     4141 cagtatcgtg aactggcagc gttctctcag tttgcatccg accttgacga tgcaacacgt
     4201 aagcagcttg accacggtca gaaagtgacc gaactgctga agcagaaaca gtatgcgccg
     4261 atgtctgttg cgcaacagtc tctggttctg ttcgcagcag aacgtggtta cctggcggat
     4321 gtagaactgt cgaaaatcgg cagcttcgaa gccgctctgc tggcttacgt cgaccgtgat
     4381 cacgctccgt tgatgcaaga gatcaaccag accggtggct acaacgacga aatcgaaggc
     4441 aagctgaaag gcatcctcga ttccttcaaa gcaacccaat cctggtaacg tctggcggct
     4501 tgccttaggg caggccgcaa ggcattgagg agaagctcat ggccggcgca aaagagatac
     4561 gtagtaagat cgcaagcgtc cagaacacgc aaaagatcac taaagcgatg gagatggtcg
     4621 ccgcttccaa aatgcgtaaa tcgcaggatc gcatggcggc cagccgtcct tatgcagaaa
     4681 ccatgcgcaa agtgattggt caccttgcac acggtaatct ggaatataag cacccttacc
     4741 tggaagaccg cgacgttaaa cgcgtgggct acctggtggt gtcgaccgac cgtggtttgt
     4801 gcggtggttt gaacattaac ctgttcaaaa aactgctggc ggaaatgaag acctggaccg
     4861 acaaaggcgt tcaatgcgac ctcgcaatga tcggctcgaa aggcgtgtcg ttcttcaact
     4921 ccgtgggcgg caatgttgtt gcccaggtca ccggcatggg ggataaccct tccctgtccg
     4981 aactgatcgg tccggtaaaa gtgatgttgc aggcctacga cgaaggccgt ctggacaagc
     5041 tttacattgt cagcaacaaa tttattaaca ccatgtctca ggttccgacc atcagccagc
     5101 tgctgccgtt accggcatca gatgatgatg atctgaaaca taaatcctgg gattacctgt
     5161 acgaacccga tccgaaggcg ttgctggata ccctgctgcg tcgttatgtc gaatctcagg
     5221 tttatcaggg cgtggttgaa aacctggcca gcgagcaggc cgcccgtatg gtggcgatga
     5281 aagccgcgac cgacaatggc ggcagcctga ttaaagagct gcagttggta tacaacaaag
     5341 ctcgtcaggc cagcattact caggaactca ccgagatcgt ctcgggggcc gccgcggttt
     5401 aaacaggtta tttcgtagag gatttaagat ggctactgga aagattgtcc aggtaatcgg
     5461 cgccgtagtt gacgtcgaat tccctcagga tgccgtaccg cgcgtgtacg atgctcttga
     5521 ggtgcaaaat ggtaatgagc gtctggtgct ggaagttcag cagcagctcg gcggcggtat
     5581 cgtacgtacc atcgcaatgg gttcctccga cggtctgcgt cgcggtctgg atgtaaaaga
     5641 cctcgaacac ccgatcgaag tcccggtagg taaagcgact ctgggccgta tcatgaacgt
     5701 actgggtgaa ccggtcgaca tgaaaggcga gatcggtgaa gaagagcgtt gggcgattca
     5761 ccgcgcagca ccttcctacg aagagctgtc aaactctcag gaactgctgg aaaccggtat
     5821 caaagttatc gacctgatgt gtccgttcgc taagggcggt aaagttggtc tgttcggtgg
     5881 tgcgggtgta ggtaaaaccg taaacatgat ggagcttatt cgtaacatcg cgatcgagca
     5941 ctccggttac tctgtgtttg cgggcgtagg tgaacgtact cgtgagggta acgacttcta
     6001 ccacgaaatg accgactcca acgttatcga caaagtatcc ctggtgtatg gccagatgaa
     6061 cgagccgccg ggaaaccgtc tgcgcgttgc tctgaccggt ctgaccatgg ctgagaaatt
     6121 ccgtgacgaa ggtcgtgacg ttctgctgtt cgttgacaac atctatcgtt acaccctggc
     6181 cggtacggaa gtatccgcac tgctgggccg tatgccttca gcggtaggtt atcagccgac
     6241 cctggcggaa gagatgggcg ttctgcagga acgtatcacc tccaccaaaa ccggttctat
     6301 cacctccgta caggcggtat acgtacctgc ggatgacttg actgacccgt ctccggcaac
     6361 cacctttgcg caccttgacg caaccgtggt actgagccgt cagatcgcgt ctctgggtat
     6421 ctacccggcc gttgacccgc tggactccac cagccgtcag ctggacccgc tggtggttgg
     6481 tcaggaacac tacgacaccg cgcgtggcgt tcagtccatc ctgcaacgtt atcaggaact
     6541 gaaagacatt atcgccatcc tgggtatgga tgaactgtct gaagaagaca aactggtggt
     6601 agcgcgtgct cgtaagatcc agcgcttcct gtcccagccg ttcttcgtgg cagaagtatt
     6661 caccggttct ccgggtaaat acgtctccct gaaagacacc atccgtggct ttaaaggcat
     6721 catggaaggc gaatacgatc acctgccgga gcaggcgttc tacatggtcg gttccatcga
     6781 agaagctgtg gaaaaagcca aaaaacttta acgccttaat cggagggtga tatggcaatg
     6841 acttaccacc tggacgtcgt cagtgcagag caacaaatgt tctctggtct ggtcgagaaa
     6901 atccaggtaa cgggtagcga aggtgaactg gggatctatc ctggccacgc accgctgctc
     6961 accgccatta agcctggtat gattcgcatc gtgaaacagc acggtcacga agagtttatc
     7021 tatctgtctg gcggcattct tgaagtgcag cctggcaacg tgaccgttct ggccgacacc
     7081 gcaattcgcg gccaggatct cgacgaagcg cgagccatgg aagcgaaacg taaggctgaa
     7141 gagcacatta gcagctctca cggcgacgta gattacgctc aggcgtctgc ggaactggcc
     7201 aaagcgatcg cgcagctgcg cgttatcgag ttgaccaaaa aagcgatgta acaccggctt
     7261 gaaaagcaca aaagccagtc tggaaacagg ctggcttttt ttacgcgtga cccgtcctga
     7321 agaacgttca catagatcct gctgatataa aaccccctgt tttcccgttt attcattgat
     7381 cgaaataaga gcaaaaacat ccacctgacg cttaaatgaa ggtactgcct taattttctg
     7441 cagacaaaag gtgtgacgat ggtcgaaaat ggcgctttcg tcagcgggga taatccgtta
     7501 ttgaacaatt tatcctctgt ccatttcacg atgaaaaaaa tgtagttttt tcaaggtgaa
     7561 gcggtttaaa ttcgttctca aattacagtc aggacgcgta tgttgaataa tgctatgagc
     7621 gtagtgatcc ttgccgcagg caaaggcacg cgcatgtatt ccgatcttcc gaaagtgctg
     7681 catacccttg ccgggaaagc gatggttcag catgtcattg atgctgcgaa tgaattaggc
     7741 gcagcgcacg ttcacctggt ttacggtcac ggcggcgatc tgcttaaaca ggcgctgaaa
     7801 gatgacaacc tgaactgggt gcttcaggca gaacagctgg gtacaggtca tgcgatgcag
     7861 caggccgcac ctttctttgc cgatgatgaa gacattttaa tgctctacgg cgacgtgccg
     7921 ctgatctctg tcgaaacact ccagcgtctg cgtgatgcta aaccgcaggg tggcattggt
     7981 ctgctgacgg taaaactgga tgatccgacc ggttatggac gtatcacccg tgaaaacggc
     8041 aaagttaccg gcattgttga gcacaaagac gccaccgacg agcagcgtca gattcaggag
     8101 atcaacaccg gcattctgat tgccaacggc gcagatatga aacgctggct ggcgaagctg
     8161 accaacaata acgctcaggg tgaatactac atcaccgaca ttattgcgct ggcgtatcag
     8221 gaagggcgtg aaatcgtcgc cgttcatccg caacgtttaa gcgaagtaga aggcgtgaat
     8281 aaccgcctgc aactctcccg actggagcgc gtttaccagt ctgaacaggc tgaaaaactg
     8341 ctgttagcag gcgttatgct gcgcgatccg gcgcgttttg atctgcgcgg tacgcttact
     8401 cacgggcgcg atgttgaaat tgatactaac gttatcatcg agggcaacgt gactctcggt
     8461 catcgcgtga aaatcggcac cggttgcgtg attaaaaaca gcgtgattgg cgatgattgc
     8521 gaaatcagtc cgtataccgt tgtggaagat gcgaatctgg cagcggcctg taccattggc
     8581 ccgtttgccc gtctgcgtcc tggtgctgag ttgctggaag gtgcacacgt cggtaacttc
     8641 gttgagatga aaaaagcgcg tctgggtaaa ggctcgaaag ccgggcatct gacttatctg
     8701 ggcgatgcgg aaattggcga taacgttaac atcggcgcgg gaaccattac ctgcaactac
     8761 gacggtgcga ataaatttaa gaccattatc ggcgacgatg tgtttgttgg ttccgatact
     8821 cagctggtgg ccccggtaac ggtaggcaaa ggcgcaacca ttgctgcggg tacaactgtg
     8881 acgcgtaatg tcggcgaaaa tgctctggca atcagccgtg tgccgcagtc gcaaaaagaa
     8941 ggctggcgtc gtccggtaaa gaaaaagtaa ttctggctgg gaaatccggt catatgggat
     9001 gaggagataa cataatctcc cgcccacaag cagtaactat aaaaataacc ccactctcta
     9061 caaggctcgg ggcgcccgaa aaaacgggca tacaggttga ccgacaacga tataaatcgg
     9121 aatcaaaaac tatgtgtgga attgttggcg cgatcgcgca acgtgatgta gcagaaatcc
     9181 ttcttgaagg tttacgtcgt ctggaatacc gcggatatga ctctgccggt ctggccgttg
     9241 ttgatgcaga aggtcatatg acccgcctgc gtcgcctcgg taaagtacag atgctggctc
     9301 aggcagcgga agaacatcct ctgcatggcg gcaccggtat tgctcatact cgctgggcga
     9361 cccacggtga accttcagaa gctaacgcgc atccgcatgt ttctgaacac attgtggtgg
     9421 tgcataacgg catcatcgaa aaccatgaac cgctgcgtga agagttaaaa gcgcgtggct
     9481 ataccttcgt ttctgaaacc gacaccgaag tgattgcaca tttggtgaac tgggagctga
     9541 aacaaggcgg tactctgcgt gaggcagttc tgcgtgctat tccgcaactg cgtggtgcgt
     9601 acggtacagt gatcatggac tcccgtcacc cggataccct gctggcggca cgttctggta
     9661 gcccgctggt gattggtctg ggcatgggcg aaaactttat cgcttctgac cagctggcgc
     9721 tgttgccagt gactcgtcgc tttatcttcc ttgaagaggg cgatattgcg gaaatcactc
     9781 gccgttcggt aaacattttc gataaaactg gcgcggaagt aaaacgtcag gatattgaat
     9841 ccaatctgca atatgacgcg ggcgataaag gtatttaccg tcactacatg cagaaagaga
     9901 tctacgaaca accgaacgcg atcaaaaaca cccttaccgg gcgcatcagc cacggtcagg
     9961 ttgatttaag cgagctggga ccgaacgccg acgaactgct gtcgaaggtt gagcatattc
    10021 agatcctcgc ctgtggtact tcttataact ccggtatggt ttcccgctac tggtttgaat
    10081 cgctggcagg tattccgtgc gacgtcgaaa tcgcctctga attccgctat cgcaaatctg
    10141 ccgtgcgtcg taacagcctg atgatcacct tgtcacagtc tggcgaaacc gcggataccc
    10201 tggcgggcct gcgtctgtcg aaagagctgg gttaccttgg ttcgctggca atctgtaacg
    10261 ttccgggttc ttctctggtg cgcgaatccg atctggcgct gatgaccaac gccggtacgg
    10321 aaatcggcgt ggcatccact aaagcattca ccactcagtt aactgtgctg ttgatgctgg
    10381 tggcgaagct gtctcgcctg aaaggtctgg atgcctccat tgaacatgac attgtgcatg
    10441 gtctgcaggc gctgccgagc cgtattgagc agatgctgtc tcaggacaaa cgcattgaag
    10501 cgctggcaga agatttctct gacaaacatc acgcgctgtt cctgggccgt ggcgatcagt
    10561 acccaatcgc gctggaaggt gcattgaagc tgaaagagat ctcttacatt cacgctgaag
    10621 cctacgctgc gggcgaactg aaacacggcc cgctggcgct gattgacgcc gatatgccgg
    10681 ttatcgtcgt tgcgccgaac aacgagttgt tggaaaaact gaaatctaac attgaagaag
    10741 ttcgcgcgcg tggcggtcag ttgtatgtct tcgccgatca ggatgcgggt tttgtgagca
    10801 gtgacaacat gcacatcatt gagatgccgc atgtggaaga ggtgattgca ccaatcttct
    10861 acaccgttcc gctgcagcta ctggcttacc acgttgcgct gatcaaaggc accgacgttg
    10921 accagccgcg taacctggcg aaatcggtta cggttgagta atatctctcg ttgatagaaa
    10981 agggctgccg accggcggcc cttttttata agtgatatca ataattccat tattcatatg
    11041 cccattgata taaatatatt ctgaattatt gctaa
//
LOCUS       DAZGHF010000149        10940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGHF010000149 DAZGHF010000000
VERSION     DAZGHF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.150"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            79..1293
                     /gene="manD"
                     /locus_tag="ACHK3A_004182"
     CDS             79..1293
                     /gene="manD"
                     /locus_tag="ACHK3A_004182"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO8742193.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTASGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1360..2643
                     /locus_tag="ACHK3A_004183"
     CDS             1360..2643
                     /locus_tag="ACHK3A_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8742194.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            2895..3812
                     /gene="oxyR"
                     /locus_tag="ACHK3A_004184"
     CDS             2895..3812
                     /gene="oxyR"
                     /locus_tag="ACHK3A_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO8742195.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3795..5195)
                     /gene="sthA"
                     /locus_tag="ACHK3A_004185"
     CDS             complement(3795..5195)
                     /gene="sthA"
                     /locus_tag="ACHK3A_004185"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO8742196.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5527..6177
                     /gene="fabR"
                     /locus_tag="ACHK3A_004186"
     CDS             5527..6177
                     /gene="fabR"
                     /locus_tag="ACHK3A_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO8742197.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6177..6536
                     /gene="yijD"
                     /locus_tag="ACHK3A_004187"
     CDS             6177..6536
                     /gene="yijD"
                     /locus_tag="ACHK3A_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO8742198.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLL
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6576..7676)
                     /gene="trmA"
                     /locus_tag="ACHK3A_004188"
     CDS             complement(6576..7676)
                     /gene="trmA"
                     /locus_tag="ACHK3A_004188"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO8742199.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7790..7980
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8045..9928
                     /gene="btuB"
                     /locus_tag="ACHK3A_004189"
     CDS             8045..9928
                     /gene="btuB"
                     /locus_tag="ACHK3A_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO8742200.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     gene            9873..10730
                     /gene="murI"
                     /locus_tag="ACHK3A_004190"
     CDS             9873..10730
                     /gene="murI"
                     /locus_tag="ACHK3A_004190"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO8742201.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2565 a         2568 c         2904 g         2903 t
ORIGIN      
        1 tcgctctttc tctcttgtat ggtagtaggc gcaaaattaa gtttgaaacg tctatttaca
       61 acacaggaga ttgcaataat gaaaattatt gctgcagatg tttttgttac ttgcccaggg
      121 cgtaatttcg ttacgcttaa aataaccact gcaagcggac tttgtggatt aggagatgcc
      181 acgttaaatg gtcgcgaatt gtcggtcgct tcttatctga aagatcatct ttgtccacaa
      241 ctaattggcc gcgatgccag ccgtatcgaa gatatctggc aattctttta taaaggtgct
      301 tactggcgac gtggccccgt cacgatgtca gccatttctg ccattgatat ggcgttgtgg
      361 gatatcaaag caaaagcggc aaatatgcca ttgtatcaac tgcttggtgg tgcttctcgt
      421 gaaggcgtta tggtttattg ccacaccacc gggcgtacga ttgatgaagt ccttgaagac
      481 tatgcaaagc accagcagat gggctttaag gcgattcgtg tgcagtgtgg tgtaccgggt
      541 atgcaaacca cctatggtct ggcgaagggc aaaggactgg cgtatgaacc agcaaccaaa
      601 ggcctgtggc cagaagagca gttgtggtct tcagaaaagt atctcgattt tacgccgaag
      661 ttatttgaag cggtgcgtaa taagttcggt tttaacgaac atctgttgca tgacatgcac
      721 catcgcctga cccccatcga agcggcgcgc tttgggaaga gcattgaaga ttaccgcctg
      781 ttctggatgg aagatccgac gcctgccgag aatcaggaat gttttcgtct gattcgtcag
      841 cataccgtga cgccgattgc ggtcggtgaa gtgtttaaca gtatctggga ctgcaagcag
      901 ttaatcgaag agcagctgat tgactatatt cgcacgacga ttacccatgc aggcggcatt
      961 accgggatgc gacggattgc cgattttgca tcgctttatc aggtacgtac cggttcgcat
     1021 ggcccttcag atttatcgcc tgtttgccat gcggcggcgc tgcattttga cctctgggtg
     1081 ccaaactttg gcgttcagga gtacatgggc tattccgaac aaatgcttga agtcttcccg
     1141 catagctggc gcttcgatga aggttatatg cacccaggcg atgaaccggg gctgggtatt
     1201 tctttcgatg agaaactggc ggcgaaatat ccgtatgacc cggcgtatct ccccgtcgca
     1261 cgactggaag atggcacatt gtggaactgg taatagcggt tcgcttaatt attttctgaa
     1321 tattcatcaa atcgattaat tccgctcaag gagctaaaaa tggacgttga tgtgtcaacc
     1381 agtgttgcgg gtaataaacc gcaacggatt cgtcgtattc agacagtgac cctggtttta
     1441 ttatttatgg cggggatcgt taattttctc gaccgttcgt cattgagcgt ggcaggtgaa
     1501 gcaattcgtg gcgagttagg attatccgct accgagtttg gtgttttgct ttctgcattt
     1561 tctctgtctt atggtttttc acaacttcct tccggtattt tgctagaccg ctttggccca
     1621 cgaatagtgt taggcgcagg cttaatattc tggtcattaa tgcaggcatt aacaggaatg
     1681 gttaattctt ttagccactt tattttaatg cgtatcggtc tgggtattgg cgaagcgcca
     1741 tttatgcctg cgggagtaaa gtcgatcacc gactggtatg cgcaaaaaga acgcggcacg
     1801 gcgctgggga tttttaactc atctaccgtt atcggtcagg ctatcgcgcc tcctgctctt
     1861 gtattgatgc aactggcatg gggctggcgg acgatgtttg tcatcatcgg cgtagcaggg
     1921 attctggttg ggatctgttg gtacgcgtgg tatcgcaacc gggcgcagtt tgtcctgacc
     1981 gaggaagaac ggacatatct ctccgcgccg gttaaaccgc gtccacagtt acaatttagc
     2041 gagtggctgg cgctgtttaa gcatcggaca acctggggga tgattttagg tttctctggc
     2101 gtcaattata ccggttggct ctacatcgcg tggttacccg gttatttgca ggccgaacaa
     2161 ggtttcagtc tggcgaaaac cggttgggtg gcggcaattc ctttcctcgc ggcggcggtc
     2221 gggatgtggg ttaacggtat tgttgtcgat cggctggcga aaaaaggcta tgacctggcg
     2281 aagacgcgta aaacggcgat tgtctgcggt ttgatgatgt cggcattagg cacgttgctg
     2341 gtcgtgcaat cttcctcgcc agcccaggcg gtagcgttta tctcaatggc gctgttctgc
     2401 gtgcatttcg cgggaacatc tgcatggggg ctggtgcagg tgatggtgtc agaaacaaaa
     2461 gtggcttcca tcgccgggat tcaaaacttt ggcagttttg tttttgcttc ctttgctccg
     2521 atcgtaaccg gttgggtcgt ggataccaca cactcgttta atctggcgct ggttatcgcg
     2581 gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta ttgtcaaaga tcggattgag
     2641 taaccgttac tatcaggctc tcttgctgtg ggcctgtaga ataaaaaaaa gcggatcctg
     2701 gagatccgca aaagttcacg ttggctttag ttattcgagt tgagaaactc tcgaaacggg
     2761 cagtgacttc aagggttaaa agaggtgccg ctccgtttct gtgagcaatt atcagtcaga
     2821 atgcttgata gggataatcg ttcgttgcta tgctacctat cgccgcgaac tatcgtggca
     2881 atggaggatg gataatgaat attcgtgatc ttgagtacct ggtggcattg gccgaacacc
     2941 gccattttcg gcgtgcggca gattcctgcc acgttagcca gccgacgctt agcgggcaaa
     3001 ttcgtaagct ggaagatgag ttgggtgtga tgttgctgga gcggaccagc cgtaaagtgt
     3061 tattcaccca ggcgggaatg ctgctggtgg atcaggcgcg taccgtgctg cgtgaggtga
     3121 aagtccttaa agagatggca agccagcagg gcgagacgat gtccggaccg ctgcatattg
     3181 gtctgattcc cacggttgga ccgtacctgc taccgcatat tatcccgatg ctgcaccaga
     3241 cctttccaaa gctggaaatg tatctgcatg aagcacagac ccaccagtta ctggcgcaac
     3301 tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt gaaagagagc gaagcattca
     3361 ttgaagtgcc gttgtttgat gagccaatgt tgctggctat ctatgaagac catccgtggg
     3421 cgaaccgcga atgcgtaccg atggccgatc tggcagggga aaaactgctg atgctggaag
     3481 atggtcactg tttgcgcgat caggcaatgg gtttctgttt tgaagccggg gcggatgaag
     3541 atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa catggtggcg gcaggtagcg
     3601 ggatcacttt actgcctgcg ctggctgtgc cgccggaacg caaacgtgat ggggttgttt
     3661 atctgccatg cattaagccg gaaccacgcc gcactattgg cctggtttat cgtcctggct
     3721 caccactgcg cagccgctat gagcagctgg cagaggccat ccgcgcaaga atggatggcc
     3781 atttcgataa agtgttaaaa caggcggttt aagccattta gcgcagctac ccgataggct
     3841 tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt actcaatagt gttgccgcca
     3901 cctttctgtt ccataatcgc ctgaccgata tgaataattt cggcagcgcg ctcgccaaag
     3961 cagtgaatac ccagaatctc ttttgtttcc cgatggaaca aaattttcag cgtgcccacg
     4021 ttcatgccga cgatttgtgc acgtgccaga tgtttaaact gggcgcggcc cacttcatat
     4081 ggcactttca tcgcggtcag ctgctgttcg gttttgccca cagagctgat ttccgggatg
     4141 gtgtaaatac cggtagggat atcttcaatc agatgtgcgg tggcttcacc tttcaccagc
     4201 gcctgcgcgg caatgcgccc ctggtcatag gccgccgacg ccaggctcgg ataaccaatc
     4261 acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat acatgctgtt gaccttcagc
     4321 tgtccgcggc tgtcagtttc cagcccaatg ttctgtaacg ccagcgaatc ggtattaccg
     4381 gtgcgaccgt tggcatagag caggcagtca gctttcagtt ttttacccga cttcaaatgc
     4441 atgatcacac cgtcgtcaca gccttcgatc ttctcgtact cttcgttgtg acgaatcact
     4501 acgccactgt tccagaagtg ataggagaga gaatctgaca tctcttgatc gagaaatgcc
     4561 agcaggcgat cgcgggtgtt gatcagatcc acttttacat ccataccgcg gaagatcgac
     4621 gcatattcac agccgatcac tccagcacca tagataagta catggcgcgg ttcgtggtgc
     4681 atgctaagaa ttgagtcgct gtcgtaaatg cgtggatgcg tgaaatcaac atctgttgga
     4741 tgatatggac gagagccgca agcaataaca aatttttcag cggttagtgt ctcgacgctg
     4801 ccgtccgggc aatccagcgc cagcgtgtgc tcgtcaacaa agcgagcgtt cccctgcaat
     4861 atttcacagt gattacgttc gtaaaatccc tgacgcatgc gcgtttgttg attaatcacg
     4921 ttatcggcat ggttaaggat atcggcaaaa gaagagcgga gcagtcgcga atggtcgctg
     4981 taaagtgggt tttgattaaa ttctataatg cggctgacgg cgtgacggag agctttcgac
     5041 gggatggtgc cccagtgggt gcaaccgccg ccaacatttt gataacgctc gataactgcg
     5101 acgcgcgcac cttgcttaac caggcccatt gcagcgcctt cgccgccggg gccggaacct
     5161 attactatgg catcgtaatc gtaggaatgt ggcatggtag ggcttacctg ttcttataca
     5221 taaaagcaac agaatggtaa cattttatcg cgggtaagcc aattgatccc cgtcatttat
     5281 ctggctatat cgtgagcggc ctttgctttg tctgtttctt acttttgacc tgaacgtttt
     5341 attggatttt tatcgacgat actctccgtt taagcggcag gtttccgttg tacgtaaaag
     5401 aaccggccaa agaattgcag taaatatgtt ttattgcgtt accgttcatt cacaatactg
     5461 gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg caaggattca
     5521 gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc gctggtggaa
     5581 gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt gcgtgaagtg
     5641 gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga cgtagacgaa
     5701 ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat gcgtcaggcg
     5761 cgtcagcgta tcgccaaagg cgggagtgtg atccgcacct cggtctccac atttatggag
     5821 ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc cggcacctcc
     5881 gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga acttgcggac
     5941 tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc cgaagcaatg
     6001 gtgacgattg tcttcagtgc gggggccgag gcgttggatg tcggcgtcga acaacgtcgg
     6061 caattagaag agcgactggt actgcaactg cgaatgattt ctaaaggggc ttattactgg
     6121 tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga cgagtaatga
     6181 aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc ttatcgatta
     6241 atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc ccgattattt
     6301 ccctgttgct gacggtttac tgcctgcatc aacgttatct taatcgcacc atgccggtag
     6361 gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac agcacggtag
     6421 ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc tccgtcatta
     6481 tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct gagtaatcgg
     6541 tatcatgccg gatgctggtg catccggcat gggttttact tcgcggtcag taatacgccg
     6601 cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc gaccttgtgc
     6661 gtctggctta atgtttccag attcttgcat aacgtttccg gattgcagga gatatacaga
     6721 atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct gcgcggtggg
     6781 tcgacaaaaa tggtttcgca ctgataactc tttaagtcga ttccttgcag gcggttaaac
     6841 tcgcgtacgc cattcatcgc ctgagtaaat tcttctgccg ccatacgaat aatttgtacg
     6901 ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgaccgacgg cttagcgatt
     6961 tcggtggcta atacccgatc aaaattgcgc gccagtgcta atgaaaagtt accgttgccg
     7021 cagtacagct ccagcaaatc gccttttgag cctttggtta cgtccagcgc ccattccagc
     7081 atctgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg acggtagatc
     7141 atctctttcc ctgcgaccgg cagacgttca tcgatgtaat cctggtccag ctcgattttg
     7201 gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgctcgcag tgcatcgcgc
     7261 agggcttccg cttcctgacg ccactcatca tccagcttct tatggtacag cagggaaacc
     7321 accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg gcgcagaacg
     7381 ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgataag ttcactggcg
     7441 gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat gatgtgatac
     7501 aggtcatcgc catcgtgcca gatgcggaac tccgcacgca tccggtaatg actgaccggc
     7561 gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg caaacgtacc
     7621 actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg ggtcatgatg
     7681 tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat gtccagatct
     7741 tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc tgtagcatcc
     7801 acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctggagctg acgcgcagcg
     7861 gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg aagtcatcat
     7921 ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggctaacg tcgcatctgg
     7981 ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt gtggatgctt
     8041 tacaatgatt aaaaaagctt cgctgctgac ggcgtgttct gtcacagcct tttccgcttg
     8101 ggcacaggat accagcccgg atactctcgt tgttactgct aaccgttttc agcagccagt
     8161 aaatactgtc cttgcaccta ctgatattgt tacccgtgag gatattcaac gctggcagtc
     8221 gaaagacctc aacgatgtta tgagtcatct ccccggtgta aatatctctc aaactggtgg
     8281 aatggggacg agttcttctc tttatgttcg aggcactgaa tcgcgccatg ttttagtgtt
     8341 gattgacggt gtgccgatgg cccgtgccgg tatcgacaat gcaattgata ttagccagtt
     8401 cccggtatca cttgtacagc gtgttgaata tattcgtggg ccgcgttctg ccgtttatgg
     8461 ttctggtgcc atcggaggtg tggtgaacat tattacaatg agtaatgatg agaaagctca
     8521 gatcaacgca ggaatgggta caaatggtta tcaagcctat gatggggtaa taaataaacg
     8581 ttttggcgat acgatggtaa cagcagcagg tgcttaccag acgaccaaag gttttaacat
     8641 tcagcctggc tcaccataca gtggtgatag cgatcgcgat ggatatcgca acaaactttt
     8701 ctgggggggg ctgcagcata agtttaacga taacttctcg gggttcttcc gtggttacag
     8761 ctacacagct aataatgatt acgaccaggg ctcgatgtat ggttctgcta ctggcaacga
     8821 agaagctcag aattacaacc agtcctggga tactggccta cgctataact ccggcatcta
     8881 ttcgtctcag ttaattgcta actatcaacg actcaagaat tataactaca caaataatct
     8941 tggtcgttat gcgggagatg ccaccctgga tgacatggaa cagcgttata ttcagtgggg
     9001 taatagcatt gaggtagggc atggtgcaat tagcggcggg atcgattgga aacaggaaaa
     9061 actgacgtcc agcagcatta caaaatcaga tgattacgaa cgcgatacca ccgggttata
     9121 cctgactgga cagcaacaga tttccaatgt gactttggaa gcttctggcc gagaagatca
     9181 tgatgaacag tttggctggc atggtacctg gcagacagct gctggctggg agtttattga
     9241 tggctaccag gcaacacttt cttacggtac cagttttctt tcgccttctc ttggacagca
     9301 atacggggct gctcgctttg cttcaattta tggtcctggt attgcagcca acccaaattt
     9361 gaagccagaa gaatctaaac agtgggagat tggaattgag ggggtaactg ggactctgga
     9421 ttggcggctc tcaggatatc gttatgaaat tcagaacctg attgattata aaagcataaa
     9481 taatgtcaac cagtacatta atgtgaaatc agccacgatt aaagggctgg aatggacggg
     9541 aaatattaca accggtcctg ttgaacatcg cttgacgtta caatatgttg acccgcgcga
     9601 tgatgaaaca gataaagtgc tgtaccgtcg ggcaaaacag caggttaagt acgagttgac
     9661 tggacaagct tatggtttcg aatgggacgt atcttaccaa tatataggtc aacgatacga
     9721 caacgcttat gacgagacct ctggctcatc acatacagtt aaattaggcg gtgtgagctt
     9781 gtgggatctt gcggttgcgt atccggtcac ctctcatcta acagttcgtg gtaaaatagc
     9841 caacctgttc gacaaagatt atgagacagt ctatggctac caaactgcag gacgggaata
     9901 caccttgtct ggcagctaca ccttctgaac cacgtcccac cgtgctggtg tttgactccg
     9961 gcgtcggtgg gttgtcggtc tatgacgaga tccggcatct cttaccggat ctccattaca
    10021 tttatgcttt cgataacgtc gctttcccgt atggcgaaaa aagcgaagag tttattgttg
    10081 agcgagtggt ggcaattgtc accgcagtgc aagaacgtta tccccttgcg ctggctgtgg
    10141 tcgcttgcaa caccgccagt accgtttcac ttcctgcatt acgcgaaaag ttcgacttcc
    10201 cggttgttgg tgtcgtgccg gcgattaaac ctgctgcacg tctgacggca aatggcattg
    10261 tcggattact ggcaacccgc ggaactgtta aacgttctta tactcatgag ctgatcgcgc
    10321 gtttcgctaa tgaatgccag atagaaatgc tgggctcggc agagatggtc gagttggctg
    10381 aagcgaagct gcatggcgaa gatgtttctc tggatgcgct aaaacgtatc ttgcgtccgt
    10441 ggttaagaat gaaagagccg ccagataccg ttgtactggg gtgtacccat ttccctctac
    10501 tacaagaaga actgttacag gtgctgccag agggaacccg gctggtggat tctggcgcag
    10561 cgattgctcg ccgaacggcc tggctgttag aacatgaagc cccggatgca aaatctgccg
    10621 atgcgaatat tgccttttgt atggcaatga cgccagaagc tgaacaatta ttgcccgttt
    10681 tacagcgtta cggcttcgaa acgctcgaaa aactggcagt tttaggctga tttggttgaa
    10741 tgttgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
    10801 aatgcgccac cactgacacg gaacaacggc aaacacgccg ccgggtcagc ggggttctcc
    10861 tgagaatctc aacagagaaa agcaaaaaaa tgcttgactc tgtagcggga aagcgtatta
    10921 tgcacacccc gcgccgctga
//
LOCUS       DAZGHF010000150        10865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGHF010000150 DAZGHF010000000
VERSION     DAZGHF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.151"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            213..1106
                     /locus_tag="ACHK3A_004191"
     CDS             213..1106
                     /locus_tag="ACHK3A_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8742202.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(1137..2126)
                     /locus_tag="ACHK3A_004192"
     CDS             complement(1137..2126)
                     /locus_tag="ACHK3A_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO8742203.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQSIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(2153..3004)
                     /locus_tag="ACHK3A_004193"
     CDS             complement(2153..3004)
                     /locus_tag="ACHK3A_004193"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO8742204.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            3570..7820
                     /gene="fdeC"
                     /locus_tag="ACHK3A_004194"
     CDS             3570..7820
                     /gene="fdeC"
                     /locus_tag="ACHK3A_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO8742205.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAQPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGITVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEALVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPAKLVLDPNGTATTNEQGQA
                     IFTATTTVAATYTLTAQVSQNNGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(7945..8802)
                     /locus_tag="ACHK3A_004195"
     CDS             complement(7945..8802)
                     /locus_tag="ACHK3A_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8742206.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKSFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARMNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            9051..9920
                     /locus_tag="ACHK3A_004196"
     CDS             9051..9920
                     /locus_tag="ACHK3A_004196"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO8742207.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(10080..10673)
                     /gene="rclC"
                     /locus_tag="ACHK3A_004197"
     CDS             complement(10080..10673)
                     /gene="rclC"
                     /locus_tag="ACHK3A_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO8742208.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIIFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(10685..>10865)
                     /gene="rclB"
                     /locus_tag="ACHK3A_004198"
     CDS             complement(10685..>10865)
                     /gene="rclB"
                     /locus_tag="ACHK3A_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO8742209.1"
                     /translation="TNADDKIILKHISVSSVSASPTVLENAIADMARKYNASSWKVTS
                     MRIDNNSTATAVLYK"
BASE COUNT         2893 a         2450 c         2767 g         2755 t
ORIGIN      
        1 aaatagaaag ttaatgatgt gtgtcagaat atcggtaaga agaatggcac aatcgtgagg
       61 gtacgtttat tcataataaa tctcctgaaa gtttagaatt attttcgata cagccagatt
      121 atcattgtta ctaataatgt ggttaaaata atagcatcta tgcccccttc atgagtgtgt
      181 gtgaagagga tgcttctaca aggaaatcgc caatgctcaa agaaaacttc aacgaactgc
      241 aaatctttct tgtggtggca agagagcgaa gttttaccaa agcagcgggc aaactcggcg
      301 tctctcagtc tgcacttagc catgcaataa aggctctgga ggaaaggctc aatatccgcc
      361 tcctgacacg gactacccgc agtgtggctc ccacagaagc gggggagaga atcattgcct
      421 gccttgaacc gcgtattgac gagcttgaac aggaactgga atcacttatt caattgaacg
      481 gcacgccctc cgggaatatt cggttatctg ccggggaaca tgccgcgcga agtctggtct
      541 ggccgaagct aaaacctttt ctcagagagt atcccgaaat caatgtcgaa ctggtggtgg
      601 ataacggttt tgtcgatatt gttgaaggcc gttttgatgc cgggatacga ctgggtgaaa
      661 atgttgataa agatatggtt gcagtcagga ttggaccaga tatgcggatg gctgtggtgg
      721 gagctccggc gtattttgct gcaaaccctg cgcctgaaac gcctcacgag ctacaaaatc
      781 atcgctgcat caacatgcgt ttgccaactg ccggtgggct ttaccactgg gagttcgagc
      841 gagaagggaa accgcttcgc gttaaggttg atgggcaact gacgtgtagt ccattgccag
      901 aaagaattga tgccgcgtta tcagggtttg ggatcgcttg tgtccctgaa gacatggttc
      961 aggagtatat agagtcaggc aagcttattc aggtgttaca ggagtggtgt ccaacttttc
     1021 ctgggtatta tctttactac cccagccgca agcagcatcc gccagctttt gcgctgttga
     1081 tcgatgcgct tcgctacacc gaataacggg tcctttatcc tggacccgtt tgggtattag
     1141 cggcctacgc gtgcctgatg ttcaggcgag taacgttcac cgacgatttt aattgtttcc
     1201 agcgcctcgc ttatctgctg cgtatctttc tgcgaaagaa caatgtcagc agcggccagg
     1261 ttttcttcca gccggtgcag tttagtggtg ccagggatgg gaacaatcca cggcttttgt
     1321 gctaatagcc atgccagtgc gatttgtgca gacgttaccc ctttctccgc agccagttca
     1381 cctaacaagg tgaccagttt ttcattggct tcaatggatt gtgctgcgaa acgcggtacg
     1441 gtgctgcggt aatcatcttt gccgaaagtg gttcccggct taatcgcccc tgtcaggaaa
     1501 cctttaccta gtgggctgaa ggggacaaaa ccaattccca gttcctccag caacggcaga
     1561 atttcctgtt caggctcacg ccaccacatg gaatattcgc tttgcagggc ggtgacaggt
     1621 tgtaccgcat gtgcacgacg aatggtttgt gctcctgctt cagacaggcc gaaatgtttg
     1681 accttacctt ctgcgatgag gtctttcacc gttcctgcaa catcttcaat aggaacatct
     1741 ggatcaacac ggtgttgata cagcaggtca ataacatctg ttttcagacg acgtaatgat
     1801 ccttcaacgg cttcacggat atgttccgga cggctgttta aaatctgctg cttattatca
     1861 tcgccgaagg taaaaccgaa tttagtggca attaccacgc ggtcacgaaa cggttttaag
     1921 gcttcgccga ccacttcttc attcagatac ggaccataca cttcggcggt atcgaagaag
     1981 gtgacgccac gttcaaccgc agcacgaata agttcgatag cctgacgggt atcggtcgcc
     2041 gggccgtagc cgtggcttaa gcccatgcaa ccgagcccaa gggcggagac ttcgagtccg
     2101 gatttaccca gataacgttt ttgcattgaa tgaatacctc ttttatcgcg gcttaaacat
     2161 caagtttgcg gccagtcagc cattccacca ttgcgggatc gcggtgagag aagaacgcgc
     2221 tggttgcggt atccagggcg gtaatttgca gcatatcttc agggctgagt tcaaaatcga
     2281 gaatgttgat gttctcttcc atgcgttctt ttcgcactga ttttgccagt gaaacaatgc
     2341 ctcgttggta gatccagcgc agaacaacct ggcccacgct tttgccatac ttctcgccaa
     2401 ttgccgtgag aacaggatgc tggaacagac catttttccc ttccgcaaac ggtgcccagg
     2461 cttctggctg aatgccacga ctttgcatcc agggaacggc ctgtagctgc tgattgaaag
     2521 ggttaacttc aacctggttt accgccggaa cgacgttatt aaaagcgata aggtctgcca
     2581 gtcggtcagg atggaagtta ctgacgccaa tagcgcgaat tttaccggcc tgttgcagct
     2641 cttccatggc acgccaggct ccgtggacat cgccatatgg ctggtgaatc aagtacaggt
     2701 caacgtaatc aagttgcagt cgattcagag agcgttcgaa ctgggctttg gccccttcgt
     2761 aactggtatc ctgcaaccag agtttcgtgg tgacaaagag ttcgttacgg gcgataccgc
     2821 tttgtttcag tgcgttcccg acctggattt cattttgata agaggcggcg gtgtcaatca
     2881 gtcgataacc gctgttaata gcgtcaatga ccgctctttc gcattcggcg gcatcggtca
     2941 tctgaaatac accaaagccc aacaggggca tttcaatacc gttgttcagt tttacttttt
     3001 gcatggtgtt atccttccgt tatcgttcat gagaagcata acgtaaaggg aagagctcga
     3061 ttagacggca ggatttgtca ggggttatga acaaaattca taaatctgtt tgagggttga
     3121 gattagtcgt gcaagttcga tatccccgga atttacagtc cgatgaataa aaatgaatgt
     3181 ccataaaaaa tatattggtt tatcccagcg tatatactta cttcgacgta ctcccaatag
     3241 cagttaatta aagacgggtc attattattc actctgaata atgaattatt cactgcccgc
     3301 agagtaagaa atataactta agtatctatt taatgacttg cacaaaaagc taaattttta
     3361 cccataaata aaaatataat cccgcgccca accacctgat gagtggctac agacactgga
     3421 tatattaggt ggcggtgtac tttcttacat aaaggtattt ccttttctgt ggaaaaggaa
     3481 accgggaaat ccccggtttt tctgacaagc agacgccatt atttgtgtct gcctatgttc
     3541 gttaattcgt tcatcaggaa attatctcaa tgtcacgtta taaaacagac aataaacagc
     3601 cacgatttcg ttattcagtt ctggcccgct gcgtggcgtg ggcaaatatc tctgttcagg
     3661 ttctttttcc actcgcagtc acctttacgc cagtaatggc agcacgtgcg cagcatgcgg
     3721 ttcagccacg gttgagcatg gaaaatacta cggtaactgc tgataataac gtggagaaaa
     3781 atgtcgcgtc gcttgccgct aatgccggga catttttaag cagtcagcca gatagcgatg
     3841 cgacacgtaa ctttattacc ggaatggcca ccgctaaagc taaccaggaa attcaggagt
     3901 ggctcgggaa atacggtact gcgcgcgtca aactgaatgt cgataaaaat ttctcgctga
     3961 aggactcttc gctggaaatg ctttatccga tttatgatac accgacaaat atgttgttca
     4021 ctcagggggc aatacatcgt accgacgatc gtactcagtc aaatattggt tttggctggc
     4081 gtcatttttc agaaaatgac tggatggcgg gggtgaatac ttttatcgat catgatttat
     4141 cccgtagtca tacccgcatt ggtgttggtg cggaatactg gcgtgattat ttgaaactga
     4201 gcgccaatgg ttatatccgg gcttctggct ggaaaaaatc gccggatgtt gaggattatc
     4261 aggaacgccc ggcgaatggc tgggatattc gtgctgaggg ctatttacct gcctggccgc
     4321 agcttggcgc aagcctgatg tatgaacagt attatggcga tgaagtcggg ctgtttggta
     4381 aagataaacg ccagaaagat ccacatgcga ttaccgctga agtgaattac acgccagtgc
     4441 ctcttctgac cctgagtgcc gggcataagc agggcaagag tggtgagaat gacactcgct
     4501 ttggcctgga agttaattat cggattggcg aacctctgga aaaacaactc gatacagaca
     4561 gcattcgcga gcgtcgaatg ctggcaggca gccgctatga cctggttgag cgtaataaca
     4621 atatcgttct tgagtatcgc aaatctgaag tgatccgtat tgctctgcct gagcgtattg
     4681 aaggcaaggg cggccagacg gtttccctgg ggcttgtggt cagcaaagca actcacggtc
     4741 tgaaaaatgt gcaatgggaa gcgccgtctt tgctggccgc aggcggaaaa attacggggc
     4801 agggcaatca gtggcaagtg acgctcccgg cttatcaggc aggcaaagac aattattatg
     4861 cgatttcagc gattgcctac gataacaaag gcaatgcctc gaaacgtgtg cagacagaag
     4921 tggttattag cggagctggt atgagcgccg atcgtacggc gttaacgctt gacggtcaga
     4981 gccgtattca aatgcttgct aacggtaatg agcaaaagcc gttggtgctg tctctgcgcg
     5041 acgccgaggg tcagccagtc acgggcatga aagatcagat caagactgaa ctaaccttca
     5101 aaccggctgg aaatattgtg actcgtaccc tgaaggccac taaatcacag gcacagccaa
     5161 cactgggtga gttcaccgaa actgaagcag gggtgtatca gtctgtcttt actaccggaa
     5221 cgcagtcagg tgaggcaacg attactgtta gcgttgatga catgagcaaa actgtcactg
     5281 cagaactgcg ggccacgatg atggatgtgg caaactccac cctgagcgct aacgagccgt
     5341 caggtgatgt ggttgctgat ggtcagcaag cctacacgct gacactgaca gcggtggact
     5401 ccgagggtaa tccggtgacg ggagaagcca gccgcctgcg acttgttccg caagacacta
     5461 atggtataac cgttggtgcc atttcggaaa taaaaccagg ggtttacagc gccacggttt
     5521 cttcgacccg tgccggaaac gttgttgtgc gtgccttcag cgagcagtat cagctgggca
     5581 cattacaaca aacgctgaag tttgttgccg ggccgcttga tgcagcacat tcgtccatca
     5641 ccctgaatcc tgataaaccg gtggttggcg gtacagttac ggcaatctgg acggcaaaag
     5701 atgctaatga caaccctgta actggcctca atccggatgc accgtcatta tcgggcgcag
     5761 ctgctgctgg ttctacggca tcaggctgga cggataatgg cgacgggacc tggactgcgc
     5821 agatttctct cggcactacg gcgggtgaat tagacgttat gccgaagctc aatgggcagg
     5881 acgcggcagc aaatgcggca aaagtaaccg tggtggctga tgcattatct tcaaaccagt
     5941 cgaaagtctc tgtcgcagaa gatcacgtaa aagccggtga aagcacaacc gtaacgctgg
     6001 tggcgaaaga tgcgcatggc aacgctatca gtggtctttc gttgtcggca agtttgacgg
     6061 ggaccgcctc tgaaggggcg accgtttcca gttggaccga aaaaggtgac ggttcctatg
     6121 ttgctacgtt aactacaggc ggaaagacgg gcgagcttcg tgtcatgccg ctcttcaacg
     6181 gccagcctgc agccaccgaa gccgcgcagc tgactgttat tgccggagag atgtcatcag
     6241 cgaactctac gcttgttgcg gacaataaaa ctccaacggt taaaacgacg acggaactca
     6301 ccttcaccgt gaaggatgcg tacgggaatc cggtcaccgg gctgaagcca gatgcaccag
     6361 tgtttagtgg tgccgccagc acggggagtg agcgtccttc agcaggaaac tggacagaga
     6421 aaggtaatgg ggtctacgtg tcgaccttaa cgctgggatc tgccgcgggt cagttgtctg
     6481 tgatgccgcg agtgaacggc caaaatgccg ttgctcagcc actggtgctg aatgttgcag
     6541 gtgacgcatc taaggctgag attcgtgata tgacagtgaa ggttaataac caactggcta
     6601 atggacagtc tgctaaccag ataaccctga ccgtcgtgga cagctatggt aacccgttgc
     6661 aggggcaaga agttacgctg actttaccgc agggtgtgac tagcaagacg gggaatacag
     6721 taacaaccaa tgcggcaggt aaagtggaca ttgagcttat gtcaacggtt gcaggggaac
     6781 ttgagatcga ggccttggtg aaaaactctc agaagacggt caaggtgaaa ttcaaggcgg
     6841 atttcagtac cggtcaggcg agcctggagg tagacgccgc tgctcaaaaa gtggcaaacg
     6901 gcaaagatgc ctttacgctg acggcaacgg ttaaggatca atacggcaac cttcttcctg
     6961 gcgctgtggt cgtctttaat ctgcctcggg gcgtcaaacc gcttgcagac ggtaatatca
     7021 tggtgaacgc cgacaaggag ggtaaagcgg aactgaaagt ggtttccgtg actgccggaa
     7081 cctatgagat cacggcgtca gcaggaaatg accagccttc gaatgcgcag tctgtaacgt
     7141 ttgtggctga taagactacg gcgaccatct ccagtattga ggtgattggc aaccgtgcag
     7201 tggcggacgg caaaaccaaa cagacgtata aagttacggt gactgatgcc aataacaacc
     7261 tgctgaaaga tagcgaagtg acgctgactg ccagcccggc gaagttagtt ctggatccca
     7321 atgggacggc gacaacgaat gagcaagggc aggctatttt caccgccacc acaactgttg
     7381 cggcgacata tacactcacg gcgcaagtga gtcagaacaa cggtcaggta tcaacgaaaa
     7441 ccgccgaatc taaattcgtc gcggatgata aaaacgcggt gctcgctgca tctccagagc
     7501 gtgtagattc tctggtggcg gacgggaaga ctactgcaac actgacggtt actctgatgt
     7561 cgggtgtcaa ccccgtagga ggaaccatgt gggtcgacat tgaggctccg gaaggggtga
     7621 cagaggcgga ttatcagttc ctgccgtcga aaaatgacca tttcgcgagc gggaaaatca
     7681 cgcgtacatt tagtaccaac aagccaggta catacacatt cacattcaac tctttgacat
     7741 atggagggta tgaaatgaaa ccagtgactg tgacaattaa cgccgttcct gcagatactg
     7801 aaggcgctga ggagaaataa aaacgacgat gtaataaata ataatggcgt tgacatgcgc
     7861 agcgccatta tttactgaat acctaccctg gcaccgatta gacggtggca gggtagggta
     7921 aatcttatgt gatggagtta caggctaaac cgggcgaacg gggataaaaa tttcgcaaac
     7981 aatgttattg ctatcaattt cgacgaagtt ttcattgtaa taaaatcgct caatatcact
     8041 gccgttcatt ctggctaacc ccctggcagg taattccatc agatagaggt ttcgcgccca
     8101 ttcagggtag tgctcccact caccttcata gcggaaagag gcatacagtc ctcggttaat
     8161 tgtccactct ttcgacatct ctgaatcggt ttgtttgcat accgtaccgg caaaagtttc
     8221 aactgtgagc aaatcgaccg attttgttga tggttcgaac gtggagacga aatagatatc
     8281 cgtcttaaaa ctcttcatac atctggctaa atgatgacgt cgcagtttga cttcttcatc
     8341 cggtgattta ttcgacactt caatatcata tttaaatgaa ttaccaaaaa taggtgtctc
     8401 aggaaagttg actaatctac actccgtctt ttgctgttga cgtattaaaa aagaagggaa
     8461 gatatttgcg agatcccaat aatcacggtg gcggtattca cggggagagc agccgaataa
     8521 ctttttaaat tcgcggctga atgactgctg tgagtcaaaa tgcaaagaga gtgcaatatc
     8581 aagcatagat ttcgcggaga gccggacaag aatggcggca cgacaaagcc ttcgtttgcg
     8641 aatgtattct cccaaaggca catgcatgaa atttcggaac agaagctgga tgttgcgtct
     8701 gctgtagcca gatttctgtg cgatatcttc gattgaaata gggtgttcga gattgcactc
     8761 aatccactcc aggagctgct gtagaatctt ctgcctgatc atcacattct ccgttaattc
     8821 tttgtcatat cgcacatttt cgttatgccc tgggccctaa gtgtatcaaa aacagcaagg
     8881 gcgaaggcaa aagcactggt agcgtctgct tcgttgtccc cgaaaataga gaggtttcag
     8941 tcctacatta ttaatgaatt ttttgcataa gtgatatcaa aatccacata ctaatttgag
     9001 gttacgtttt aacgtagact cattgttcat gcctaatgga gggactgaca gtggaatttt
     9061 ccgtattaag taacaatctg aaaatgccga tgatgggatt tggtgttttt caggttaccg
     9121 ataaaaacgt gtgcaaacag tcagtgttga acgctatccg cacgggttat cgactcatcg
     9181 acactgcagc ggtatacggt aatgaagatg ccgtcggtga agccgttcgt gaagctatat
     9241 ctgaaggttt atgtacccga gaagagttat ttataacgtc taaattgtgg gtgcaggata
     9301 tgttgaatca ggatattgca gcagcaggta ttgaagcatc attaaaaaaa tcgggactag
     9361 aatacttcga tctctattta ttgcaccagg ccatgcgcga ttattttagc gcgtggcgtg
     9421 cactcgaaga tgcctatgaa gagggcaaat taaaagcgat tggggtttcc aatttctacc
     9481 ctcatgttct ggcgaacttt tgtgaaacgg taagagttaa accgatggtc aaccaggtcg
     9541 agttgcatcc ctattttgcc caaccagagg cgctggcaac catgaagtat tataacgtgc
     9601 agcctgaagc atgggctccg ttaggtggtg gacgacataa accctttgaa aataatctgc
     9661 ttcagagtat tgcagatgcc catcaaaaat cgatttctca ggtcattctg cgttggaata
     9721 ttcaacgggg agtggtcgtt attccgaaat cgacacatca acagcgtatc gaagaaaatt
     9781 ttgctatctg ggatttctca ctgacagaga aagaaatggc gcaaattagt tcgcttgatt
     9841 tgggttatgt tggggaatcg gtaaaacatt ttaatcctga gtttgttcgt ggttgtcttg
     9901 ctgtaaaaat acatgattga tattaatcac ttattttacc tgagacgaca agaatctttt
     9961 aacaggggag tgatattgat cttcactctg tcatatctcc ggtaatatgg cgtcaggctt
    10021 catcatcaga aagggggcct gacgcctgaa aaagtgaaca acagacagtg ttcggattat
    10081 caatattcag tttttaacgt tgaactggat tcattactgc gttgtttaag aatatcccgc
    10141 gccgaatctg ccattatcat gacagcacct gccagcatca gagtatcttt caataccaga
    10201 cgaccagcac cggataaata agggaaacca tgatgagcgt cacccaatgc gggtacccat
    10261 gcctccgggg tggtgattaa aaatgagagt gttaccagcg gcgtggtaaa tgccatcagc
    10321 ccacccaata aacctaacca gcgattgaca ggattagcca aaaccagcag agcaataatc
    10381 acctccacga cgccaagacc gttggaaaaa ccataggtat tattagccgt ttgccatgct
    10441 cttgcttctg gtttgtattc gccttcgtga gtcagatgct gtttatagtc ttccgggtgt
    10501 tcataaaaga acgacattag tggactgttt gcgacgaatg gtgtaatgct gtctgcctcg
    10561 taagggacaa actttaataa cccaatccac ataaaaataa tcgcaatact cagacgaatc
    10621 aatgtcaggc caattttatc tccccgactt aacaggtgca ggtatctttc cattgtaaac
    10681 atccttattt atacaatact gctgttgcgg ttgaattatt atcaattcgc atcgatgtaa
    10741 ctttccagga tgaagcatta tattttctgg ccatatcagc aatggcattc tcaagaactg
    10801 tcggtgatgc tgatactgat gagacgctga tatgtttcag aattatttta tcatctgcat
    10861 tggtg
//
LOCUS       DAZGHF010000151        10862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGHF010000151 DAZGHF010000000
VERSION     DAZGHF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.152"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            88..849
                     /gene="adiY"
                     /locus_tag="ACHK3A_004199"
     CDS             88..849
                     /gene="adiY"
                     /locus_tag="ACHK3A_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO8742210.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSKIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            986..2323
                     /gene="adiC"
                     /locus_tag="ACHK3A_004200"
     CDS             986..2323
                     /gene="adiC"
                     /locus_tag="ACHK3A_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO8742211.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2427..4070
                     /gene="eptA"
                     /locus_tag="ACHK3A_004201"
     CDS             2427..4070
                     /gene="eptA"
                     /locus_tag="ACHK3A_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO8742212.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKITPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4067..4735
                     /gene="basR"
                     /locus_tag="ACHK3A_004202"
     CDS             4067..4735
                     /gene="basR"
                     /locus_tag="ACHK3A_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO8742213.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4736..5836
                     /gene="pmrB"
                     /locus_tag="ACHK3A_004203"
     CDS             4736..5836
                     /gene="pmrB"
                     /locus_tag="ACHK3A_004203"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO8742214.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQNLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5812..5901)
                     /gene="pmrR"
                     /locus_tag="ACHK3A_004204"
     CDS             complement(5812..5901)
                     /gene="pmrR"
                     /locus_tag="ACHK3A_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO8742215.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6013..7515)
                     /gene="proP"
                     /locus_tag="ACHK3A_004205"
     CDS             complement(6013..7515)
                     /gene="proP"
                     /locus_tag="ACHK3A_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO8742216.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7779..8657)
                     /gene="rdcB"
                     /locus_tag="ACHK3A_004206"
     CDS             complement(7779..8657)
                     /gene="rdcB"
                     /locus_tag="ACHK3A_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO8742217.1"
                     /translation="MTKTLLDGPGRVLESIHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDAFRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8654..10862)
                     /gene="crfC"
                     /locus_tag="ACHK3A_004207"
                     /pseudo
     CDS             complement(8654..10862)
                     /gene="crfC"
                     /locus_tag="ACHK3A_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
BASE COUNT         2732 a         2849 c         2658 g         2623 t
ORIGIN      
        1 tattcatcat acaattatat aaccatttcc cggtatcgct ttgctttagc gagaactggt
       61 cttttattcg cactcaggag tacatgtatg aggatttgca gcgaccaacc ttgtattgtt
      121 ttattgactg aaaaagatgt ctggataagg gtgaatggga aagaacctat tagccttaaa
      181 gctaaccata tggcgttatt aaattgtgaa aataatatta tcgacgtctc ctctcttaat
      241 aacactttgg ttgctcatat tagtcacgac atcatcaaag attacctccg gtttctgaat
      301 aaagatctct cgaaaatacc agtatggcaa cgtagcgcta cgcccatcct cacccttcca
      361 tgcctgacgc cagacgtctt tcgcgttgcc gcgcaacata gcatgatgcc cgctgaaact
      421 gagtcagaaa aggaacgaac acgtgcatta ttattcactg tgctatcccg ttttctcgac
      481 agtaaaaaat tcctttcact aatgatgtat atgttacgta attgtgtaag tgacagcgtt
      541 tatcaaatta ttgaaagcga tatccacaaa gactggaatc ttagtatggt agccagttgt
      601 ttatgtctta gcccaagtct gttaaagaaa aagctgaaaa gcgaaaacac cagttatagc
      661 caaataatca ccacctgccg catgcgctat gccgtaaatg aattaatgat ggacggtaaa
      721 aatatctctc aggtatcaca gtcctgcggc tacaacagta cgtcgtactt tatttctgtc
      781 tttaaagact tctacggtat gacgccgctg cattatgtta gtcagcacag agaacgcact
      841 gtcgcctgat ttttaacctt aacgaagagc tatattaata acggcatcag cgataacccg
      901 gccgataata attcaactat cgaatgcagg cgtatgatac gacgtaattt attgtcacga
      961 agctcgcctt cgcaggagtt taattatgtc ttcggatgct gatgctcaca aagtgggctt
     1021 aatccccgtc accctgatgg tgtcggggaa tattatgggg tcaggtgttt ttctgttacc
     1081 tgcaaacctg gcctccactg gcgggattgc catttatgga tggttggtga cgattatcgg
     1141 tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc ctcgacccaa gtcctggtgg
     1201 ttcttacgct tacgcccgcc gctgctttgg cccgtttctc ggttatcaaa ccaacgtcct
     1261 ctactggctg gcctgctgga tcggcaatat cgccatggtg gtcattggcg taggatattt
     1321 aagttacttc ttcccgattc tgaaagaccc attggtatta accatcacct gcgtcgtggt
     1381 gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc gcgtacaggc
     1441 cgttgctacc gtgctggcgc tgatccccat cgtcgggatt gccgtatttg gctggttctg
     1501 gttccgtggt gaaacctata tggcggcatg gaacgtcagc ggtctgggca ccttcggtgc
     1561 gattcaaagt acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa gtgcctccgt
     1621 tgccgcaggt gtggtgaaaa acccgaaacg caacgtccct atcgccacca ttggcggggt
     1681 attgattgct gccgtttgct atgtactttc taccaccgcg attatgggga tgatccctaa
     1741 tgccgcactg cgcgtttctg cttcgccatt cggtgatgcc gcacggatgg cgttgggtga
     1801 caccgccggg gccattgttt ccttctgcgc agctgcgggt tgcttaggtt cactgggcgg
     1861 ctggacgttg ctggcgggtc aaacggcgaa agccgctgcc gatgacggac tgttcccacc
     1921 gatttttgcc cgtgttaata aagcgggcac gccagtggcg gggctgatta tcgtcggtat
     1981 tttgatgacc atcttccagc tcagtagcat ttcgccaaac gcgaccaaag aattcggtct
     2041 ggtttcttcc gtgtcggtca tctttacact ggtgccatat ctttacacct gtgcggcgtt
     2101 actactgctc ggccacggtc actttggtaa agcacgcccg gcatacctgg cagtgactac
     2161 cattgccttc ctctactgca tctgggccgt ggtagggtcc ggcgcgaaag aggttatgtg
     2221 gtcatttgtc actctgatgg taatcaccgc catgtatgcg ctgaattaca accggctgca
     2281 taaaaacccg tatcccttag atgcaccaat aagcaaagat taattcccct taatcaagca
     2341 aacataaaag ccaaccttaa gaacttaagg ttggcttaat tttgctttgc gagcatatgc
     2401 gcactttgtt cgatggaaac accgtgatgt tgaagcgcct actaaaaaga ccctctttaa
     2461 atttattctc ctggttatta cttgctactt tttatatctc agtctgcctg aatatcgcct
     2521 tttttaaaca ggtgctacag gtgttaccgc tggattcact gcataacgtg ctggttttct
     2581 tgtcgatgcc ggtcgtcgca ttcagcgtga ttaatattgt cctgacacta ggctctttct
     2641 tatggcttaa tcgtccactc gcctgcctgt ttattctggt cggtgcggct gcacaatatt
     2701 tcatcatgac ctatggcatc gtcatcgacc gttcaatgat caccaatatc attgatacta
     2761 cgccggcgga aagttatgcc ctgatgacgc cacgaatgtt attaacgctg gggttaagtg
     2821 gcgtgttagc cgcgattatt gcctgctgga tcaaaatcac ccccaccacc tcacgcctgc
     2881 gcagcgttct tttccgcggg gccaacatcc tgatttctgt actgctgatt ctgctggttg
     2941 ccgcactgtt ctacaaagac tatgcctcgc tgtttcgcaa caacaaagag ctggtgaaat
     3001 ccttaagccc gtccaacagc attgttgcta gctggtcgtg gtactctcac cagcggtcag
     3061 ccaacctgcc actgatacgc attggtgaag atgctcaccg caacccatta atgcaaaatg
     3121 ggaaacgtaa aaacctgacc attttgatcg tcggagaaac gtcgcgcgca gagaatttct
     3181 cgctgaatgg ttatccacgc gaaaccaatc ctcgtctggc aaaagataac gtggtctatt
     3241 tccctaatac cgcctcttgc ggcacggcaa cggcagtttc agtaccatgc atgttctcgg
     3301 atatgccgcg tgagcactac aaagaagagc tggcacagca ccaggaaggc gtgctggata
     3361 tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc tgtaaaggcg
     3421 tttgcgatcg cgtacctcac cagaacgtca ccgcgctgaa tctgccaggt cagtgcatca
     3481 acggcgaatg ctatgacgaa gtgctgttcc acgggctgga agagtacatc aataacctgc
     3541 agggtgatgg cgtgattgtc ttacacacca tcggcagcca cggtccgacc tattacaacc
     3601 gctatccgcc gcagttcagg aaatttaccc caacctgtga cactaacgag atccagacct
     3661 gtaccaaaga gcaactggtg aacacttacg acaacacgct ggtttacgtc gactatattg
     3721 ttgataaagc gattaatctg ctgaaagaac atcaggataa atttaccacc agcctggttt
     3781 atctttctga ccacggtgag tcgttaggtg aaaatggcat ctatctgcac ggtctgcctt
     3841 atgccatcgc cccggatagc caaaaacagg tgccgatgct gctgtggctg tcggaggatt
     3901 atcaaaaacg gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa acgcaacact
     3961 attcacaaga taatttattc tccacgctat tgggattaac tggcgttgag acgaagtatt
     4021 accaggctgc ggatgatatt ctgcaaactt gcaggagagt gagtgaatga aaattctgat
     4081 tgttgaagac gatacgctgt tattgcaggg attaattctg gcggcgcaaa ccgaaggcta
     4141 cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa agccttgagg ccggtcatta
     4201 cagcctggtg gtactggatt tagggttacc cgacgaagat ggactgcatt ttctcgcccg
     4261 tatccggcag aaaaaataca ccctgccggt actgatcctc accgctcgcg atacgctgac
     4321 cgacaaaatc gccgggctgg atgtcggtgc cgatgactat ctggtgaagc cttttgcgct
     4381 ggaagagtta cacgcccgta tccgcgccct gctacgacgc cataataatc agggcgaaag
     4441 tgagctgatt gttggcaatc tgacgctgaa tatgggtcgc cgtcaggtat ggatgggcgg
     4501 tgaagagttg attctgacgc ccaaagaata tgctctgctg tcacggttaa tgctcaaagc
     4561 aggcagtccg gtgcatcggg aaattctcta caacgacatc tataactggg acaatgaacc
     4621 ctcgaccaac accctggaag tgcatatcca caatctgcgc gacaaagtgg gcaaagcccg
     4681 tatccgcacc gtgcgcggct ttggctatat gctggtcgca aatgaggaaa actaattgaa
     4741 tctgatgcgt tttctgcgcc gaccaatatc gctgcgccaa cggctgatat tgaccattgg
     4801 ggccattttg ttggtgtttg agctgatcag cgtcttctgg ctatggcatg aaagtaccga
     4861 gcagattcag ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc gccacatcat
     4921 gcgtgagatc cgcgaggcgg tcgccagcct gatcgtcccc ggcgtcttta tggtcagcct
     4981 gacgctattt atctgctatc aggcggtacg ccgcatcacc cggccgctgg cggagctgca
     5041 aaaagagctg gaagcgcgca ccgccgacaa cttaacgcct attgccattc acagcgccac
     5101 cctcgaaatc gaagcggtgg tttcggcgtt gaacgatctg gtcagtcgcc tgaccagcac
     5161 gctggataac gaaaggttgt ttaccgctga cgtcgcgcac gaactgcgaa cgccactggc
     5221 gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat cacattgatg tagcaccgtt
     5281 agtggctcga cttgatcaga tgatggagag tgtctcccag ctgctgcaac tggcgcgtgc
     5341 cggacagtca ttttcttccg gtaattatca acatgtaaaa ctgctggaag atgtcattct
     5401 cccctcgtat gacgaactca gtaccatgct cgaccagcga cagcaaaacc tgctattgcc
     5461 agagagcgcc gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga tgttattgcg
     5521 aaacctggta gaaaacgccc atcgttacag cccgcaaggc agcaacatta tgattaagct
     5581 gcaagaagat ggcggagcgg tcatggccgt tgaagatgaa ggaccgggta ttgatgagag
     5641 taaatgcggg gagttgagta aagcgtttgt acgtatggac agccgttatg gcgggattgg
     5701 tctggggtta agtattgtca gccgcattac acagttgcat cacgggcagt ttttcctgca
     5761 aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg ctgaagaaag atcagaacgt
     5821 ggcaaaccag atataaagaa agctgctgac caccagcacg ctgaacacgg tagttaaact
     5881 ttcataaaca cggtttttca ttgcactctc ctccgttaac ctggaggaga gtatgcgcgt
     5941 cagagattaa gtgaacctta agagttcaat tggcctgata agacagcgtc acatcaggcc
     6001 atccgtttca gcttattcat caattcgcgg atgttgctgc accaggcgag tacgtttcgc
     6061 ctgcaaatcg gcaatctcgt ggtcaatatc atcgattttc tgctcgatat tatcgtaatg
     6121 ctcgacgaga atttccttcg cttcctgtat atctgacgcc gccggtgtcg cacctttcaa
     6181 cggacgattt gccgtctctt tcatggttac gccggtgatt aaaccaacca ccgccactac
     6241 catcaggtaa taggcaggca tcatcagatt ctgcgagctt tcgaccagcc aggccgccag
     6301 tgttggcgtc agaccggcaa ccagcaccga aatattaaat gccgccgcca gcgcgctgta
     6361 acggatatgc gtcggaaaca tcgctggcaa ggtagaagcc ataacgcccg taaagcagtt
     6421 aaggatcacc gccagcatca gtaacccggc aaaaatcagg ccgatgacgt tactgttaat
     6481 cagaataaac gccgggatcg ccaacacaaa cagtgcaaca ctaccaagta gcacaaacgg
     6541 acgacggcca aaacggtcac tcagcaagcc catcaccggc tggacaaaca gcataccgat
     6601 cataatggcg ataataatca gcaccccgtg gtcttcggaa taatgcaggt tatgcgacaa
     6661 ataactcggc atataggtca gcaacatgta gtaagtcacg ttggtggcaa ttaccagacc
     6721 aatacatgtc aacaggctgc gccagtgttt ggtggcaatc tctttaaacg agactttcgg
     6781 gccatcctgc aaaccttcgc ggtcgccctg ctccagttta tcaacatgct gctggaacgc
     6841 cggggtctct tccagcgcat ggcgcaggta aagcccgata atccctaacg gcagagcaat
     6901 aaagaacgga atacgccagc cccagtcgag gaagttcgct tcgccgacaa tggtcgaaat
     6961 taacaccacc acgcccgcac ccagcacaaa cccggcaata gaaccgaagt ccagccagct
     7021 gcccataaag ccacgtttac ggtcagggga gtattccgca acaaatatcg acgccccggt
     7081 atattcaccg ccgaccgaga aaccttgtgc catcttacag atcaacagca gaatcggtgc
     7141 ccaaatacca atcgtgtcgt aggacggtat taagccaata cagaacgtac tgatcgacat
     7201 aatcacaata gtgatagcga ggatcttctg gcgaccatat ttatcgccca acataccaaa
     7261 gaagagtcca ccaagcggtc gaatcagaaa gggaacggag aaagtggcaa gtgcagcaac
     7321 catctgcacg ctggggtcag cccccgggaa aaaaacttta cctaatgcgt aagcaacaaa
     7381 accataaaca ccaaaatcaa accattccat tgcattaccc agtgatgctg cggtaatggc
     7441 tttacgcagt ttaccgtcat caataatggt gacatcacga agggtaattg gttttacttt
     7501 tttccttttc agcatagctt tcctcgcaga gttggcccat ttccgcaaac gccagaaaaa
     7561 tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga tgcaatctct gtaacaagcg
     7621 tagcgggttt gcttacactc tccggttaag gaatgtacaa tcaaaccagt tgacgcctga
     7681 attgggcagt taatgacctt tttaccctac cagcgtttaa atttgtgatc aacttcacac
     7741 atggatttcg tttaggactc attgatgtaa ctgattattt acaccaaatt tacagagaag
     7801 atttcctcga ccattgcccc cgccagacgc tgcgctgaac agagtcgtgg catccccagt
     7861 gcgacagttc gccagcgttg ggtcactgac cagcaaacgg gttgtttatc tgccgtggac
     7921 cactttccca gccaacttaa aaaatcagca tgatccccgc ccgtcggaat ggtcgaagag
     7981 gcaaaccact gatggtaata gtgacgcgtg gtcggggccg cattgatact ctctgccaga
     8041 tgttgtgtcg ctttcacgcg caaatcaccc agcaacaacg ccaccgcttg cgggcttccc
     8101 agacgaaagg catcgccaaa tgccccccaa cgcaactcct ccagaacgat aaaacagcgc
     8161 ccaggcaacg accaggcgtt gtacgcccca gcacgccagc gggtaaaaat ttgctcgctc
     8221 tgttcacccg cctgcaccag taggcctcgt tgcgttaacg ccttttcctt ccagcgacaa
     8281 cgggctgaaa accactcata caaggcggca atttgctggg ggagctcagg gaaagcagag
     8341 tgctggcggt gatccatttt ttgcagcagc gccagatgtt gcgcgatctg cgtcagcgcc
     8401 agatgaccgg gatccagcat cgacgccagt ttttccacca atgtcagacg caggctaagc
     8461 ggcgcattta acatcccgcc gttcgtccat gcctgaagct gcacacgcgc aagaagttcc
     8521 tgcatcagtc gttcacgaaa ctgctgctga tgggcttgtg gatggcgggc atcatcgccc
     8581 tgcgccagat ccactaaaaa gcggggatga atcgactcca gcacgcgacc ggggccgtcc
     8641 agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca tcgcgtaaca
     8701 gctgagcgtc ggtgtaaatc cagccatgag tcatcacagt ctgctgcaat tgctgctgca
     8761 tcagcctgac caccgattca ttttgttgac gcagagccag actttcgcgt aaacgcgtct
     8821 gtaattccgt caaacatgaa gcgaactcag cgaaaaaagt attcatgcct gccgtaacag
     8881 attcatcgac ctgctctgcc agaactttag ccatttgttg gcaataaaga tcgacttctg
     8941 cgcttaatgc tcgttgcaac gcactgtaat caaccgtctc tatcggggat ttctcatttc
     9001 cccgtcccca gtcgggctga ttcaaccagc gcgaaaaagt ctcacgcaca acgcctaaac
     9061 gcgtgctctg ctcgtccatt gcgtcctggc gcgaaatgac ttcactgaac agctggcgag
     9121 tgttgaagtg gggaactacg ccgtgaaaaa caggaaaatg aaacccagga cgaaaccctg
     9181 actcgctcaa ttccattttg atttgttgct caatggggcg aataacatcg gttaacaccc
     9241 gggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact aatggtgttc
     9301 tgcgccatct gtaacagtgt ctcacagcag gtacgaatct cgcttaatga ccctgaatca
     9361 tcctgaaaca aggcggctaa ctgcgcattc agcgcatctt gctgttgacg cagaaagtgg
     9421 ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac ctgcgcctga
     9481 ttgagttgca atagctgcaa actttcttcg atctgatgga tattttgccg caattgttca
     9541 caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg ctgcgcgtaa
     9601 ttcaacagtt tatgcgcggc agatcgcaga gcatacaacg aggcgttagc gtaagcggca
     9661 tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg atcggcagca
     9721 tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc gagcgcggca
     9781 tgggcaaaat cttccaccca gcgttgttgc tctggcggtg gtaacttacc gttgttggct
     9841 aactcatggc gcacccgatt cgccaggtag ccccacatcg acgacaccgg aaatatctgc
     9901 tgtggcgtaa tacaaccttt catcagcgtc ccggaaatca gtgcccgcac ctggtcggcg
     9961 tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacaa cggcaccgat
    10021 tgccccaccg ccaaaattgc ctcacggacc tcttcatcgg agatcgattt cagttgcgta
    10081 taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag cattttttgc
    10141 agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa ctgaccggga
    10201 taactctcca gccccgccag atggacaaac tccacttcaa tcacgggaat atgctcaatg
    10261 gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg caccaaatca
    10321 ttcaaacttt tcagacaatg aaaaataggt tgagcaccca gataatattt ttcgaaagcg
    10381 atgccatttt caatccgctg cataagcgca cgcatatctt tatctatttc cagcacatcg
    10441 gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat taaatggtcg
    10501 attggcgcga catgtgaaaa atgcagtacc ggttcttttt gcccgggcgt atggcgaata
    10561 agcgttggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt accaacaatg
    10621 gcattaatgg tggttgattt ccctgctttc atggtaccga caatcgccag caccatttcc
    10681 agccgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc gatattaggc
    10741 tgactccagg gtaaagccag ttgtggcgta tcgtctccgg gtacagagag aggtattttt
    10801 tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc ctgacttaac
    10861 tc
//
LOCUS       DAZGHF010000152        10833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGHF010000152 DAZGHF010000000
VERSION     DAZGHF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.153"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(117..1604)
                     /gene="yeeO"
                     /locus_tag="ACHK3A_004208"
     CDS             complement(117..1604)
                     /gene="yeeO"
                     /locus_tag="ACHK3A_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO8742218.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVVMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1765..1840
                     /locus_tag="ACHK3A_004209"
     tRNA            1765..1840
                     /locus_tag="ACHK3A_004209"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1798..1800,aa:Asn,seq:gtt)
     gene            complement(1878..2828)
                     /gene="cbl"
                     /locus_tag="ACHK3A_004210"
     CDS             complement(1878..2828)
                     /gene="cbl"
                     /locus_tag="ACHK3A_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO8742219.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2930..3847)
                     /gene="nac"
                     /locus_tag="ACHK3A_004211"
     CDS             complement(2930..3847)
                     /gene="nac"
                     /locus_tag="ACHK3A_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO8742220.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4174..4249
                     /locus_tag="ACHK3A_004212"
     tRNA            4174..4249
                     /locus_tag="ACHK3A_004212"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4207..4209,aa:Asn,seq:gtt)
     gene            complement(4304..5239)
                     /gene="ldtA"
                     /locus_tag="ACHK3A_004213"
     CDS             complement(4304..5239)
                     /gene="ldtA"
                     /locus_tag="ACHK3A_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO8742221.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5301..6380)
                     /gene="cobT"
                     /locus_tag="ACHK3A_004214"
     CDS             complement(5301..6380)
                     /gene="cobT"
                     /locus_tag="ACHK3A_004214"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO8742222.1"
                     /translation="MQILADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAILVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPISGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPRDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6392..7135)
                     /gene="cobS"
                     /locus_tag="ACHK3A_004215"
     CDS             complement(6392..7135)
                     /gene="cobS"
                     /locus_tag="ACHK3A_004215"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO8742223.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLVKILVLSELTLRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVVIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7132..7677)
                     /gene="cobU"
                     /locus_tag="ACHK3A_004216"
     CDS             complement(7132..7677)
                     /gene="cobU"
                     /locus_tag="ACHK3A_004216"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO8742224.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WNYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7811..8248)
                     /locus_tag="ACHK3A_004217"
     CDS             complement(7811..8248)
                     /locus_tag="ACHK3A_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO8742225.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLNGGSVQSLSDAYSDYSVLLLIAAESEISVPEWLPHSEGTTVIVIAEHNARLLTGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(8466..8641)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8730..9644)
                     /gene="pocR"
                     /locus_tag="ACHK3A_004218"
     CDS             complement(8730..9644)
                     /gene="pocR"
                     /locus_tag="ACHK3A_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFO8742226.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSQSDSVRQTRAPNPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSENAAMETV"
     gene            complement(9847..10656)
                     /gene="pduF"
                     /locus_tag="ACHK3A_004219"
     CDS             complement(9847..10656)
                     /gene="pduF"
                     /locus_tag="ACHK3A_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFO8742227.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVTGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
BASE COUNT         3043 a         2878 c         2310 g         2602 t
ORIGIN      
        1 actgaactac agaggaatcg tgagaacgag gcgaatatta gcgatgccca tccacaatgt
       61 caaagcctgt tttttaaatt tgaaatcgtt tgctgaaata atctgcattt tgtcgtttat
      121 tccgacacaa ctggcttttt ttcacacttt tgcggctcgg gtcgagggta tttccacagc
      181 caacgtccag taaccattcg ccagtaaaac agcacggccc gcacagccca gtcggcaaac
      241 attcccatcc agacaccaac cacaccccag ccaagcatga ttcccagcac ataaccgact
      301 acaacccggc aaccccacat gctcaacata gaaacccaca tggcgtaacg tgcatcgcga
      361 gcccccttaa atccagcggg tagcacccat gaggcggacc aaataggcat aaataaagca
      421 tttagccaaa tcagaatcac aacgacgtgt ttaacctgtg gatcctgggt gtaaaacgat
      481 gccataaccc cggcaaaggg agccgttagc caggcgatgg ccgttaatcc aagagtggac
      541 agccagaaca catgccgcaa ctgaatctct gcttgcgcta tctgccctac tcccaacctt
      601 cggcctgtaa tgatcgtaga ggcagaaccg agcgcacttc cgggtaagtt gataagagcc
      661 gcaattgaga acgcgataaa atttccggca ataacgctgg tccccatccc ggcaacgaac
      721 atttgagtta ataaccgacc actggtaaat aacactgatt cgacactcgc tggaatacca
      781 atccccatga cttcccagat aatgctaaaa ttcagcggtt taaaatagct ctttaacgaa
      841 atccttagcg caggattaaa accaatcgcc agcacccaca aaattgcaac tgcgccaata
      901 taacgagaaa tggttaaacc caacccagcc ccgacaaatc ccagtcccgg ccaggagaaa
      961 aggccgtaaa tcaatatgcc gctaataata atattaagaa tattcaggct accgttaatc
     1021 agtagcggta ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat aagagtgatg
     1081 gcagctgctg gataactgag tacggtcagc tccagataag tcaacgccag tgctttaact
     1141 tctgtcgtgg catcacccgc gacgaaatca ataatttgtt cgccaaaatg atgaattagc
     1201 gttgccaaca gtacggcaaa caacgtcatg atcaccaatg actgccgcgt cgccaccctc
     1261 gctcgtcgtc gatctcgctt accgagacta aatgctacaa cgacagtagt accaagatcg
     1321 atagctgcaa aaaaagccat aacgaccata ttgaagctgt ccgccaaccc cacgccggcc
     1381 atcgcatctt ttcccagcca gctgaccaga aaagtgctca ggacccccat caacaggaca
     1441 caggcgttct ccatgaagat aggaacagca agcggggtta tctcgcgcca gaacaacact
     1501 ttgtagctct tgcgtttaga gtgccagcga gtgccgtgaa caacctggcg taaagcagag
     1561 gagatattca aagccgacct taattgcaga aagtgaaacc acatttcaaa tgatgaggga
     1621 gaatcagcaa agctgcaaag attttcgcca acaaattgtc tgcaaatgca acaaactgtt
     1681 gatagaaacg gcaaacagtt gggaaattta aaaatcgggt ttgacaaaat atttttcgcc
     1741 gttaagatgt gcctcaacag cgattcctct gtagttcagt cggtagaacg gcggactgtt
     1801 aatccgtatg tcactggttc gagtccagtc agaggagcca aatttctgaa aagcccgctt
     1861 ttatagcggg ctttttgcta tatctgataa tcaatttcct cttcactgtt ttccatcacc
     1921 tggcgcttga tatcctcaac tgacagtcct gcattacaaa gttccagaaa gcgccagaca
     1981 tagttacgct gaagttgccc tcgcttcagt cccaaccaga cagtattagc atcgaaaaga
     2041 tgccgcgtat ccaggcggat taaattcttt tcctcttgtt cgccgctgga ttgttcggca
     2101 actaatccga tcccaagccc aagagcaaca taggttttaa tgacatcaga atcctgtgca
     2161 cttaatacaa tatctgccgg caaacctttg cgggcaaatg cgtcatcaat gcgtgagcgc
     2221 cccgtaattc cctgtcggta agtaattaac ggccacttcg ctattgattc cagcgtcaat
     2281 ggtgtaattt gcgttaaggg atgatcgagt ggaacaagca aactatggtg ccagcgaaac
     2341 cacgggaagg cgacgagctg cgggtcatta ctcaaacgct cgctggcgat accaatatca
     2401 gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg taccctggat tagctcgagc
     2461 cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct ctggcaagct ataacgtgcc
     2521 tgagtatgcg tcgttgcaat agtgagaaca ccagacgtat cgttggtaaa caggtctgca
     2581 agccgacgaa cattactggc ttcattcaga atacgttctg caatgaccag taatgctttg
     2641 cccggttcag tcatgcccag cagtcgctta cctcgtcgaa caaatatttc gatgccaagt
     2701 tcatcctcca gttcccgaat atgacggcta acgcctgact gtgaggtaaa aagcatattc
     2761 gcaacctctg tcaggttgta atcctgacgt gcagcctcgc ggattatctt tagttgttgg
     2821 aaattcacgg taaactccgg gcagttcaga tatcccgtta ttgttaaagt ctaatgcccg
     2881 gcataacaaa taataaaaac ccgcatctta ttccatcccg atataacgct tagctcacca
     2941 attgccactg ccttttttcc atcactggag aactaatcac tgacattaac aactctttca
     3001 ctgcctgtgc ctgtggtgac aagtttgctc tggcgggtaa atttaaggac aaagagagac
     3061 tcattgaagg agtggtaatg cgtgacatcc acccatttac tgcgccacat aacgaacgcg
     3121 cagccgattc aggtaatact gcaacgccca tgccgctggc aatcgctgcg gtaagcgtgg
     3181 caatagactc aatttcacca atgacttttg ccgtgagccg ccgtagggaa aaagcctcat
     3241 caacacgaag tctaacagcg ctgtaatcac tggggagaaa aaggttcatt tgcgcaatag
     3301 cattaacatc aacgctttgc cccgggcaat cttgagttcc taccaggaaa agatcttctt
     3361 tcagcaaagc ctgactggat accccagcca caggggaatg ctcataaatc accgccatat
     3421 cgagttggtg atttatcagt ttttcgttaa gcactgcacc actattttca tgaagataga
     3481 taacgacctc cgggaattca gcgcgaaccg cctgtaataa gggcatggtg atggatgacg
     3541 cagcggttcc tggcgcaaag ccaatcgaga cttgccccga taatgactga ccaacgttat
     3601 gcaccgccag ttgggcctgc tcacactgac gtaaaatggc ccgtgcatgg gtatagagaa
     3661 tttttccggc gtctgttggc gtaacgcccc gttttgtgcg aatcaaaagc tgttgattta
     3721 actcaccttc cagtgtggca acctgctggc tgagcgctgg ttgtgcgata tgcaatactt
     3781 cagcagcctg ggtcaggcta ccaatatcta caatttttac gaagtatttc aggcgtctga
     3841 agttcatgtt gcctccggtt tttaagaatc ggcccaagtg ccgccattac ttacaaccag
     3901 aatgcaagat gcttgccagt tctattttag cgttgatgcg caagctaacc aactgtcaaa
     3961 taagagatta tgatagattc gtcattggcc ctttttatca gcagtcgcat tcacctgccc
     4021 tataaaagga gggtatgcac cacgatggtt cattacccaa taagattgaa agctcaccac
     4081 tttgttgaaa ttggcagcaa acaaacaaaa aaatgcattt caccctttga catcaccatg
     4141 cactgccatt aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg
     4201 cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa attcaaaaaa
     4261 gcctgcttcg agcaggcttt ttgctttcta attaccaacg ctcttaaaac atctgtcttg
     4321 aaccagaact aatttgcaca ggcattcccg atcgatgttg caacgcagca tttgcgcgat
     4381 ttacatcaac ttcttgcccg ttgataaacg cccgcaaaga tggggttacc ggcaatggca
     4441 cttttcggtc agactcatat tctgcacgat tgcgcgacag cggctcatga acttccagcc
     4501 actttgaacc atccggttcc gttgtgtatt tcactggctg atcaataatt tgtacacgag
     4561 tgcctacagg aacattatca aacagatatt tgatatcgtc attgcgcaga cgaatacagc
     4621 cctgacttac ccggagccca ataccaaaat tggcattggt accgtggatg gcatacaacc
     4681 tgccaatata aatcgcgtac agccccatgg ggttatcggg ccccgcagga acaaatgcgg
     4741 gcaaactctc ccctcgtttc gcatattcgc gccgagtgtt cggcgttggc gtccaggttg
     4801 gtgcttcttg tttacgttca acggtagtca cccagttacg cggggtttct cgcccagcct
     4861 ggccgatacc aataggaaag acttccactg tattactgtc tggcggatag taataaagac
     4921 gcatttcagc gacgttaacc acaatccctt tacggacagt ggcgggcaaa atcagttgct
     4981 gcggaatggt gagttgtgag ccagacttcg gcaaaaaaac atcagcgccc ggattcgctt
     5041 ccagcatatt acttaaccct tgcccgtatt gtgcggcaaa agtctccagc ggctgagtat
     5101 tgtgatcagg aacagttaca gtaagcgact gccccactaa acggctaccc tctggaggta
     5161 atggataagt taccgccagg ctattttggc tggcaaaaag cagagcaaat gagcaaagaa
     5221 tatttacacg acgcatcatg tccctttcct aagtcgcgaa tactatccga taagtatagc
     5281 ttttatcaga cttttcgttt ttagctgttc aaatcagaag tcgtattccc cggtagaaca
     5341 atattactgg cagcaagttc gcccatattg ttgtatatcg cacaagcagc ttcgatgatg
     5401 ggcatcgcca gagctgcacc actcccctca cctaaacgca tatccatatt gagataaggc
     5461 tccagcccca aatgcgacag cgctatacgc gcgccttttt ctgccgacaa gtgagaagga
     5521 atgagatacg gtttgattgc aggagacatc tggcaggctg cgagcgcagc agcataagaa
     5581 agaaatccat ccagcaacac gggtaaaccg caggaagcag cacctaacat cactccagct
     5641 attccgacca aatcaaatcc acccactttt gccaggacat caacaccatc ccgaggattt
     5701 ggttgattca acgtaatcgc ccgacgcaca acatcaattt tattagccag tttatctgtc
     5761 ggcaggtttg cgccaatccc aaccacttct tcaggatccc ggccagtgat tgtgctgact
     5821 attgccgctg ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa cagcgtgaca
     5881 ccgttttttg ccagctcccg cgtataacat atgacgtcca aaagcaactt ttcagcctga
     5941 cggcgactca ttgccggagc tgaagcaata ttgccgctac ctcgtgcgac acgcatgttg
     6001 ataagcccgg agataggctc agcagtatca ataccaacat caattacgtg gacgttagcg
     6061 cccgcttgtg ctgccagcac acacacgccg gttgttccgc gggtcatatt ttcagcctgt
     6121 atggctgtca cttcttttgg agaaatagcg accccttcct cccagacgcc gtgatcggca
     6181 cacataacca gtatcgcttt tttgcccaca tgcggtatgc cattcaaccc cggcattcct
     6241 gccagttgta tggcaagcgc ctccagcttt cccaggctac caacaggttt gagtaacccg
     6301 tcaatatgcc gttgtgcacg cgacatagcg gcaggatcga tggctgggat cgtattcagt
     6361 aaatcggcaa gtatttgcat gtcacgtctc tttatagcag tgccagcagg aaaaccagtt
     6421 caccaagctc tatggctgct cccagcgtat cgcccgtttg cccgcccagc gtacgtttga
     6481 gaagctgacc gaggatgaaa atcaccacca tcgtcaccac catagcagcc acaccatgca
     6541 tacccggcaa taatacggca gcaaaaatag cggctaagcc gagtgtgacg caggtttgtc
     6601 gcccatcaat tttgccaatg aacacattgc caagcccttc ttcccgcgca taacgatgac
     6661 gatacatcaa taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc agcgatgcaa
     6721 ggatcggttc gccacgcaga gtcaactcgc ttaacaccag aatctttacc agtaccacaa
     6781 aaatcaatgc cagaccgccg tgggtgccta aacggctatc acgcatgatt tccaacatgc
     6841 gatcgcggct acgtgcagaa aatacgccat cacaggtatc agcgaggccg tccagatgga
     6901 atcccccggt catcagcgcc agcacaagta cgctaaacag tgccgccagt ggtacgccac
     6961 accatgcctg cagcaccatg aagaccagcc cgctaatcgc gccaagcaat aatccaatca
     7021 aaggaaaagt aataataccg cgagaataat gctcgaaatc cagtccctgg gaccagcgac
     7081 gcggtacagg cagacgcgta ataaatgaga gcatcgccca aaataattta ctcatttgat
     7141 ttttactcca atacccgaaa ccaccagcca tacttcattt gccgcagcgg ccaactgctg
     7201 atttacccgc ccggcaatat cacgaaaatg tcgtgccaga cgactctccg gcacaatccc
     7261 cattcccact tcgttagtca ctaatacaac ctttgcgggg caacgttggc aggcagcaat
     7321 caacgactga atctcagcat taatcgcctg ttccatcgcc tgataattcc attcatcagg
     7381 gtctttatcg ccgccataat caaacaacag attagtcacc attgtggtaa cgcattccag
     7441 caacacagcc tcattcgggt taatgtctgc atgaattaat tcatcaagat gttgccaacg
     7501 ctccactgtg cgccagtgct ccgggcggcc ttgccgatga tgttctatcc gtgcagccat
     7561 ctcatcatca aggatttgcg aggtggcgat atacagaacc tgtgaagagt ccccaataag
     7621 cgcctctgcg tggcgactct tcccgctccg tgcgcccccc gtcaccaaaa tcatcatacc
     7681 gactcctgat tcaattgtat gatggtattc attgaacaga acaatggatc ccccaccgtt
     7741 gctgaccgca aaacatttac gcgattaata ctgcgcgtaa tataattaaa aatccagcat
     7801 tctcaatcca ttacgaaaga agatggcatt gcggaatagc cagacttcct cgtgttaacg
     7861 tccgacaaaa tgcgtcattg acagcggttt ctctggcatg acagatttca ttcagataat
     7921 ggatcaatgc cacagcccca ccagccggac cagtcagcaa acgggcattg tgttctgcaa
     7981 tcacaattac cgtcgttccc tcagaatgag ggagccattc tggtacggat atctcgcttt
     8041 cagctgctat caatagcagc actgaataat cgctatacgc atctgataat gattgcacag
     8101 acccaccgtt aagaggctgg aaatcttgct gccagtcctc accaacaaaa caggttattg
     8161 gcaatatttc agccagatgt ttcgccaggt ccacatctcc agataagagg caaaaaagag
     8221 ctatcgattc gggttttatc gttttcatgc ctctcgccct ccacttagtt tcctgtaatg
     8281 tcggtctggt tgatgagcca tgaaacaaga ttgccacacc ttaagtgcag ccctggtgcc
     8341 tgtcggcact gcgatgaaag aactaaatga tgccgccata ccctgatcct ttcagggtgg
     8401 taggggtata aagacgcgcc ggtactctac cggacgcttc cgggcaaccc acttcccacc
     8461 gaagttgttg gtattgactg acagactggt cttctggctt agcgtcatcc tcccccgacc
     8521 ttcccaatct cgcgatcagt ggtattttcg ggtttgtcag cattacagca gcgggggctg
     8581 cgagggattt tcaccctctt cccaggcaca aatgtgccac acctgtcagc gtatgttatg
     8641 agctgatttc acttttccca taacacgtaa ataacgttaa ttattcattc cagcgtttat
     8701 ttaaaccttc ccttttttta gcatttactc tataccgttt ccatcgccgc attttcactc
     8761 acttgctgtc gatacacctg cggcgtaacc tgataagtct gacggaaaac tttgcaaaaa
     8821 taactggttt gcgaaaaacc taaattacgc gcaatgctgg cgatgctcca gtcactatgg
     8881 cacagtaact cacgagcgct ggccatccgt tgttggttta cccacgcatt aaaaccgatt
     8941 ccatggtatt tcttgaataa cttactgaaa taataaggac ttagataaac gtgcgaggcg
     9001 acatcttcca gacgcaaatc gtctgataaa tgcgcatcaa tatagcgtaa agcttttttc
     9061 attttattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ctgagatttg
     9121 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     9181 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     9241 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     9301 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccaactaa atgtccggcg
     9361 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     9421 tcggatttag aggcttccag acccccacaa cgatcgctca tgcgacatcg agtgctcagc
     9481 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     9541 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     9601 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     9661 taatttattg ttatagcaaa acaattgttt tacatttaat cttcacatca tccttgatga
     9721 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
     9781 aataagttcg gaaagcagat cacaaacagt aaaaaaataa acgtttataa ctgccagatt
     9841 atttcgttat tcgctgattt ctttaggcgt tacactattg acaggtaaat tctttgcaat
     9901 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
     9961 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tccccccaac ccgcaaacca
    10021 ggtgaaaatt ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
    10081 ggtagaagca ccaataacag caaccagaat accaatgagt aaaggcgcaa gcggtccttt
    10141 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
    10201 gaccacttcg accaacgctg cttgccatac attaatcgcc gctgctggat aagtactgaa
    10261 gatactggcg agttgtaaac tttctgcgct accacgcact atattgtgag cagattcaaa
    10321 atcggtaaac aaactgctgt agagagaata tgccagaacc gcaccaccaa aggcacccgc
    10381 taattgcgcc acacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
    10441 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
    10501 aagcgaaatc cctaatcccc aaatgataca aatttcccac agtcccagac ttgccccggt
    10561 aaccttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
    10621 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttctt tacggagttt
    10681 ttctttatca gccaactatg cgctgattgt tgtgtatacc gagcgcaact ataaaaaatt
    10741 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
    10801 acaaattatt gtcattgccg aatcatgcaa cac
//
LOCUS       DAZGHF010000153        10752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGHF010000153 DAZGHF010000000
VERSION     DAZGHF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.154"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            40..588
                     /locus_tag="ACHK3A_004220"
     CDS             40..588
                     /locus_tag="ACHK3A_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO8742228.1"
                     /translation="MNVNKKKLAEIFNVDIRTIDRWQTQGLPQISGGGKGVEVIFDSA
                     KAIEWYAQREADIENEKLRKEVEDLRAASESDLQPGTIDYERYRLTKAQADAQELKNA
                     REEGLVLETELFTYIFQRVAQNISGILVRVPQTLQRKYPEISPAHLDAMKTEIAKASD
                     VASEAGENVRRWIDDFRRTEGG"
     gene            560..2488
                     /locus_tag="ACHK3A_004221"
     CDS             560..2488
                     /locus_tag="ACHK3A_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO8742229.1"
                     /translation="MISDELRAANSAGAIATGLLALKIPVPLTTVQWADRHYYLPKES
                     SYTPGRWETLPFQVAIMNSMGNDRIRTVNLIKSARVGYTKMLLGVEAYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEPTIRDVSVLLDLAPWFGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2472..2678
                     /locus_tag="ACHK3A_004222"
     CDS             2472..2678
                     /locus_tag="ACHK3A_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO8742230.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2675..4267
                     /locus_tag="ACHK3A_004223"
     CDS             2675..4267
                     /locus_tag="ACHK3A_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8742231.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYHGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRSRFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETRPRET"
     gene            4257..5762
                     /locus_tag="ACHK3A_004224"
     CDS             4257..5762
                     /locus_tag="ACHK3A_004224"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO8742232.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALSAEVCQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEKLAAFPGMTVEAARDILS
                     SSPDKAEPVSASTTAMFERIMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            5799..6146
                     /locus_tag="ACHK3A_004225"
     CDS             5799..6146
                     /locus_tag="ACHK3A_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO8742233.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
     gene            6204..7232
                     /locus_tag="ACHK3A_004226"
     CDS             6204..7232
                     /locus_tag="ACHK3A_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO8742234.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPTYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTSAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7284..7667
                     /gene="gpFI"
                     /locus_tag="ACHK3A_004227"
     CDS             7284..7667
                     /gene="gpFI"
                     /locus_tag="ACHK3A_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO8742235.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMHSDESGSDQNDTDDGEPLNNTDAPDDVKAVRVRKCLHVMGYCPETGRPVELT
                     FRGMRVLVPSSLAMAMIQHGTAEYA"
     gene            7660..8013
                     /locus_tag="ACHK3A_004228"
     CDS             7660..8013
                     /locus_tag="ACHK3A_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO8742236.1"
                     /translation="MRDVQNAFDAALVGVDSTIVEVMGIRVQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            8029..8604
                     /locus_tag="ACHK3A_004229"
     CDS             8029..8604
                     /locus_tag="ACHK3A_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8742237.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTTINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            8601..8996
                     /locus_tag="ACHK3A_004230"
     CDS             8601..8996
                     /locus_tag="ACHK3A_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO8742238.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAAIPALAGMITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            9004..9753
                     /locus_tag="ACHK3A_004231"
     CDS             9004..9753
                     /locus_tag="ACHK3A_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8742239.1"
                     /translation="MATPNPLAPVKGSGTTLWVYTGKGDAYANPLSDDEWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQSTTLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            9770..10192
                     /locus_tag="ACHK3A_004232"
     CDS             9770..10192
                     /locus_tag="ACHK3A_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8742240.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAEQQGTTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            10219..10632
                     /locus_tag="ACHK3A_004233"
     CDS             10219..10632
                     /locus_tag="ACHK3A_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO8742241.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPVCEEEQTEMPDEEDMLMQKAAGVAGGVRFCGVGGGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            10613..>10752
                     /locus_tag="ACHK3A_004234"
     CDS             10613..>10752
                     /locus_tag="ACHK3A_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8742242.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQG"
BASE COUNT         2499 a         2562 c         3339 g         2352 t
ORIGIN      
        1 atttcatgtc gtaactgatt tttgataagg aattgatgaa tgaatgttaa taaaaagaag
       61 cttgctgaaa tttttaatgt cgatatacgc actattgatc gctggcaaac tcaggggctc
      121 cctcagatat ctggtggagg aaaaggtgtt gaggtcatct ttgattctgc gaaggcgatc
      181 gaatggtatg ctcagcgcga agctgatatt gaaaatgaaa aactccgtaa agaggtcgag
      241 gatttgaggg ctgccagcga atcagacctt cagcccggca ccattgatta cgaacgttac
      301 cgactgacga aggcacaggc agatgcacag gagctgaaaa atgctcgtga ggaaggcctt
      361 gtcctcgaga cggagttatt tacctacatc tttcagcgag tggcacagaa tatatctggg
      421 atccttgtcc gtgtccctca gacactgcag cgtaaatacc ctgagatatc acccgcacat
      481 cttgatgcta tgaaaactga aatcgcgaaa gcatccgatg tggcttctga agccggtgag
      541 aatgtgcgca ggtggattga tgatttcaga cgaactgagg gcggctaatt ctgcaggagc
      601 gatagcaacc ggccttcttg cgctaaaaat tcctgtcccc ctgacgacag ttcagtgggc
      661 agatcgacac tattaccttc cgaaagagtc atcttacacc ccggggcggt gggaaacact
      721 gccatttcag gttgccatca tgaacagcat ggggaatgac cggatccgca cggttaatct
      781 gattaaatct gcccgtgttg gttatacaaa gatgttgctg ggagtggagg cttattttat
      841 tgagcataaa tcacgcaaca gccttctttt ccagcctacg gattctgctg ctgaagattt
      901 tatgaaatct catgtggaac ccacgatcag ggatgtgtcg gttttactcg atcttgcacc
      961 gtggtttggg cgtaaacatc gtgataatac cctcacgctg aaacgctttt catcgggtgt
     1021 gggtttctgg tgcctgggtg gtgcggcagc aaaaaactac cgtgaaaaat ccgtggatgt
     1081 ggtctgctat gacgaacttt cctcgttcga gccggatgtt gaaaaagagg gctcgccaac
     1141 tctgctgggg gataagcgta ttgagggctc ggtatggcca aaatccattc gcggctcgac
     1201 gcctaaaata aaaggcacct gccagatcga aaaagcggcc aacgagtcgg cgcatttcat
     1261 gcgtttttat gtgccctgcc cgcactgtgg ggaggagcag tatctgaaat ttggcgatga
     1321 atccacgcct tttgggctta aatgggagaa ggacagtccc gaaagtgttt tctacctctg
     1381 tgaacatcat ggctgcgtga tccatcagtc tgagcttgac cagagcaacg ggcggtggat
     1441 ctgtgaaaac acggggatgt ggacccgtga cggtctgacg tttttcagcg ctgcgggtaa
     1501 tgaaattccg ccgccgcgct ccatcacatt ccatatctgg acggcgtaca gtccgttcac
     1561 cacctgggta cagattgtct atgactggct ggatgcactg aaagatccca acggcctgaa
     1621 aacttttgtg aacaccacgc tgggcgagac ctgggaagag gccgtgggcg aaaaactcga
     1681 tcaccaggtg ctgatggata aggttgtgcg ttacacggcg gcggtacctg cccgggtggt
     1741 ttatctgacg gcgggcattg actcgcagcg taaccgtttt gagatgtatg tctggggatg
     1801 ggctccggga gaggaagcct ttctggtgga taaaatcatc attatggggc gtcctgatga
     1861 ggaagagact ctgttacgtg tggatgcggc gatcaacaaa aaataccgcc atgcagacgg
     1921 aaccgaaatg accatttccc gtgtctgctg ggacaccggg gggatcgatg gcgaaatcgt
     1981 ttatcagagg tcaaaaaaac acggtgtttt ccgggtgctg ccggtaaaag gcgcatctgt
     2041 ctatggcaag ccggtgatca ccatgccaaa aacccgcaat cagcggggcg tgtatctgtg
     2101 tgaagtgggg acggacaccg caaaagaaat tctctatgcc cgtatgaaag ccgatcccac
     2161 gcctgcggat gaagccacgt cgtatgccat ccgttttcct gatgatccgg agattttttc
     2221 gcagacagag gcgcagcaac tggtcgcgga agagcttgtg gagaagtggg aaaaaggaaa
     2281 gatgcgtctg ctgtgggata acaaaaagcg gcgtaacgaa gcgctggact gcctggtgta
     2341 tgcctacgcg gcattacgtg tgtccgtgca acgctggcag cttgatctgg ctgtactggc
     2401 aaaatcccgg gaagaagaga cgacccggcc aaccctgaaa gaactggcag cgaagctgtc
     2461 cggaggagtg aatggttaca gtcgctgaac tgcaggcgct gcgtcaggcg cgccttgatt
     2521 tattaaccgg taaacgggtg gtgtctgtcc agaaagatgg tcgcagaatt gaatatacgg
     2581 cggcttctct ggatgagctt aaccgtgcga tcaatgatgc ggagtcggta ctggggacaa
     2641 cccgacgtcg ccgtcgtccg ctgggagtga ggttatgaaa cgaacgcctg tcctgattga
     2701 tgtgaacggc gttccgcttc gggagagcct cagctaccac gggggcggtg caggatttgg
     2761 tgggcaaatg gcagagtggt tgccaccctc gcagagtgcc gatgcggccc tgctgcccgc
     2821 gttgcgtctg gggaatgccc gtgcagatga tctggtacgc aataacggga tagcggctaa
     2881 tgcggtggcc ctgcataagg atcatattgt cgggcatatg tttctgatca gctaccgtcc
     2941 gaactggcgc tggctgggga tgcgggagac tgcggcaaaa agctttgtcg atgaggtgga
     3001 ggcggcctgg tcggaatacg ccgaagggat gtttggcgag atcgacgtgg aaggaaaacg
     3061 cacgttcacg gaatttatcc gtgaaggcgt gggcgttcat gcgtttaacg gcgaaatctt
     3121 tgtgcagccg gtctgggata cggaaaccac gcagttattc cgcacgcgtt ttaaagccgt
     3181 gagtccgaaa cgggtggaca cgccaggaca cggtatcggg aatcgttttc tgcgggccgg
     3241 ggtggaggtc gatcgatatg ggcgtgccgt tgcgtaccat atctgtgagg atgattttcc
     3301 tcgctccggg agtggacgat gggaacggat cccgcgtgaa cttcccaccg ggcgtccggc
     3361 catgctgcat attttcgagc cggtggagga cgggcagacc cgtggggcca atcagtttta
     3421 cagcgtcatg gaacggctga agatgctcga ttccctgcag gcaacacagc ttcagtcagc
     3481 cattgttaaa gccatgtatg cagcgacgat tgaaagtgaa cttgataccg aaaaggcctt
     3541 tgaatatatc gcgggggcac cgcagggtca gcaggataat ccgctcatta atattctgga
     3601 gaagttctcc agttggtatg acacgaatca cgtgacgctg ggcggtgtca aaattccgca
     3661 ccttttcccc ggtgatgatc tgaaactgca gaccgcgcag gattcagaca atggattttc
     3721 tgcgcttgaa caggcgctgc tgcggtatat cgccgccggt cttggcgttt cctacgaaca
     3781 gttgtcccgt gattactcga aggtcagtta ctcaagtgcc cgcgcctccg ccaatgagtc
     3841 gtggcgctat tttatggggc ggcgaaaatt tattgcggcc cggctggcca cgcagatgtt
     3901 ttcctgctgg ctggaagagg cacttcttcg ggggattatc cgtccgccac ggtcacgttt
     3961 tgatttttat caggcgcgat cagcctggtc acgggcagag tggattggtg ccggaagaat
     4021 ggccattgac gggctcaagg aggtccagga atcagtgatg cgcattgagg ccggactgag
     4081 cacgtatgag aaagagctgg cgctgatggg tgaggattat caggacattt tccgccagca
     4141 ggtcagggaa tccgcagagc gggaaaaagc cggactctca cgtccggtgt ggatagcaca
     4201 ggcgtatcag cagcagatag cggagagccg caggccggaa gaggagacaa gaccacgtga
     4261 gacgtaatct ttcacacatt attgccgcag cattcaatga accgctgctt ctggagcccg
     4321 cctatgcgcg ggttttcttt tgcgcgctcg ggcgcgagat gggggcagca agtctttcgg
     4381 taccacaaca gcaggtgcag cttgatgctc ccggaatgct ggctgaaacg gacgagtaca
     4441 tggccggagg taaacgaccg gcccgtgttt accgggtggt gaacggtatt gctgtactgc
     4501 cggtgaccgg cacgctggtg caccggctgg gtggcatgcg gccattttcc ggaatgacag
     4561 gctatgacgg cattgttgcc tgtcttcagc aggcaatggc ggacacctct gtccggggcg
     4621 tactgctgga tattgacagt ccgggcgggc aggccgccgg tgcgtttgac tgcgctgaca
     4681 tgatttaccg cctccggcag cagaagccgg tctgggcact gtgtaatgac acggcctgtt
     4741 ctgcggccat gctgctggcg tcggcctgct cccgacggct ggttacccag acatcccgta
     4801 tcggctccat tggcgtgatg atgggccatg tcagctatgc cggtcatctg gcgcaggccg
     4861 gggtggatat cacgctgatt tatgccgggg cgcataaggt ggatggcaat cagtttgaag
     4921 ccttatcggc agaggtgtgt caggacatgc agcagcgcat tgatgcggcg caccggatgt
     4981 ttgccgaaaa agtggcgatg tatacggggc tgtctgtgga agctgtcacg gggacagagg
     5041 ctgccgtttt tgaaggtcag tccggtattg aggccgggct ggcggatgag ttaatcaatg
     5101 cgtcggatgc catcagcgtg atggctgcgg cgctgaacac acatgataca ggaggcacta
     5161 tgccgcaatt aactgcaacg gaagccgccg cgcaggagaa ccagcgagtg atggggatcc
     5221 tgacgtgtca ggaagcgaaa ggacgtgaac agcttgccac gatgctggca ggacaacagg
     5281 gcatgagcgt tgaacaggcc cgggcgattc tggctgcggc agcaccacag cagccggtgg
     5341 catccgcgca gagtgaagcc gatcgcatta tggcgtgtga agaagctaaa ggtcgtgaac
     5401 aactggcggc aacgctggcg gcgatgccgg agatgacggt ggaaaaagcc cgcccgatcc
     5461 tggcggcttc accgcaggcg gatgccggac cctcactccg tgatcagatt atggctttgg
     5521 atgaggcaaa aggggctgag gcgcaggctg aaaaactggc ggcgtttccc ggaatgacgg
     5581 tggaggctgc ccgcgacatt ctgtcctcat cgccggataa agcagaaccg gtttctgcat
     5641 ccacaaccgc catgtttgaa cgcatcatgg cgaaccattc accggcagcc gtgcagggtg
     5701 gcgtgtcaca gacgtcggca gacggtgatg cggacgtgaa aatgctcatg gccatgccat
     5761 gaagtcagtg ctgaccatca atatgaggtt tttacaatat ggtaacgaaa accatcactg
     5821 aacaacgtgc agaagtacgt atttttgccg gtaatgatcc ggctcatacc gccacaggca
     5881 gcagcgggat ttctcaggca acaccggcac tgacgcccct gatgctggat gaagccagcg
     5941 ggaaactggt ggtctgggac ggacagaaag ccggtagtgc ggctggcata ctggtactgc
     6001 cgcttgaagg cacagagacg gtgctgacgt attacaagtc gggaaccttt gcgacggagg
     6061 caatccgctg gcctgaaagt gtggatgaac acaaaaaggc caacgccttt gtcggcacag
     6121 ccctgagtca cgcggcgctg ccgtaacacg ttatcaggcc accgcgttgg cctgactgat
     6181 ttctgaatga aaggaactga tttatgggat tgtttacgac ccgccagtta ctcggttata
     6241 ccgaacaaaa agtgaaattt cgtgcgctgt ttctggaact gtttttccgc cgtacggtga
     6301 atttccacac cgaagaggtg atgctggaca aaattaccgg aaaaacgccg gtggcagcct
     6361 atgtctcccc ggttgttgaa ggaaaagtgc tgcgtcatcg tggtggtgaa acccgcgtgt
     6421 tacgtccggg ctacgtcaag ccgaaacacg aatttaatta ccagcaggcg gtggagcgcc
     6481 ttcccggtga agatccggcg cagctgaacg acccgaccta ccgtcgtctg cgtatcatca
     6541 ccgataacct caaacaggaa gagcacgcca ttgtccaggt ggaagaaatg caggcggtga
     6601 atgccgtgct gtatggcaaa tacaccatgg aaggggagca gtttgatact gtcgaggtgg
     6661 atttcgggcg ctctgaagga aataacattg agcaggctga cggtaaaaaa tggtctgagc
     6721 aggaccgtga tacgtttgat ccgacgcatg atattgacct ctactgcgat caggccagcg
     6781 gccttgtgaa tatcgccatt atggacggta cggtctggcg tctgctgaat ggctttaagc
     6841 tgttccgcga aaaactggat acccgtcgcg gctcaaattc acaactcgaa acggcagtga
     6901 aagatctggg cgcagtggtg tccttcaagg ggtattacgg cgatctggcc attgtggtgg
     6961 cgaaaacgtc ttatgtggca gaggacggta ccgaaaaacg ttatctgccg gagggcacgc
     7021 tggtcctggg aaatacgtca gcggagggca tccgttgcta tggtgccatt caggatgcgc
     7081 aggcgttgtc cgaaggtgtg gtggcctctt cccgttatcc gaaacactgg atgaccgtgg
     7141 gcgatccggc ccgtgaattc accatgacgc agtccgctcc gctgatggtg ctgccggatc
     7201 cggatgagtt tgtggtggta caggtgaaat aatccgggag cgggggcgaa atgcccccgt
     7261 gtcttttttc acagggggct gagatggcaa caaaagaaga aaatctgaat cgtcttcgtc
     7321 aactggctgg tctgctgggg cgcgaggcgg atatgtcggg gagtgctgcg gatattgctc
     7381 agcgtgtgtc tgagtgggaa gaggagcttg ctgcttcccg ggagggcatt atgcactctg
     7441 atgagagcgg gtctgatcaa aatgacacag acgatggtga gccgttgaac aacacggatg
     7501 ctccggatga tgttaaagcc gtccgtgtgc gcaagtgcct gcatgtgatg gggtattgcc
     7561 cggagacagg ccgtcctgtt gagcttacgt tccgggggat gcgtgttctg gtgccatcat
     7621 cactggcaat ggccatgata cagcacggaa cggctgagta tgcgtgatgt tcagaatgcc
     7681 tttgatgccg cccttgtggg ggtggacagc acgattgttg aagtgatggg gatccgtgtg
     7741 cagtttacct ccggagcaca acgtggcggt gaagttcagg gggtttttga cgatccggag
     7801 tcgctggggt ttgccggtgg cggggtccgt attgaaggaa gcagcccgtc attatttgtg
     7861 cggacggata cggtgcgtgc cgtgcggcgt ggtgacacgc tgaccattaa cggcgagatg
     7921 ttctgggtgg atcgtgtttc tccggatgac gggggcagct gttatctctg gctcaaccgt
     7981 gggcaaccac ccgctgttaa ccggcgacga taaacgcagg gtgaaattat ggcgataaaa
     8041 gggcttgatc aggcgattga taatctgagc cgggttcgta aaaacgccat tccggcggct
     8101 tcagcaatga cgattaaccg cgtggccaca acgacgatta atcagtcttc atcacaggtt
     8161 gcccgggaga caaaggtacg ccggaaactg gttaaggaac ggtccagact gaaacgggcc
     8221 acggtcagaa atccgaatgc cagaattatc gttaaccgcg gtgatctccc tgttattaag
     8281 ctggggatca gaatgctggg gcgtcgtccg gacagcatac tcaaagccgg tcagcatcgt
     8341 tatcagcggg catttatcca gcgattaaat aatgggcgct ggcatgttat gcaacgtctt
     8401 ccccaggcca gatatgagaa gggcaatgac gacaagggca ggaaaaagcg taatcgcctt
     8461 cccattcagg tggtgaaaat cccgatggcg gccccactga aacaggcgtt tgatgaaaac
     8521 gttgaccgta tccgtcgtga acgcctgccc aaagaactgg catatgcgct gaaacaacaa
     8581 ctgaggattg cgataaaacg atgaaacata ctgatatccg tgcagccgta ctggatgcac
     8641 tggagaagca tgacaccggg gcgacgctgt ttgatggtcg ccccgctgtt tttgaggagg
     8701 cggattttcc ggcggtcgcg gtttatctga cggatgcaga gtataccggt gaagagctgg
     8761 atgctgatac ctggcgggcc acactgcata ttgaggtgtt tttaccggca caggtaccgg
     8821 attcagagct tgatcagtgg atggaaagcc ggatttaccc ggcgatggcg gcgatccctg
     8881 cactggcagg catgattacc acgatggtta cgcagggcta tgagtatcgt cgtgatgacg
     8941 atatggcatt gtggagctct gcagatctga cttattccat tacatacgag atgtgaggat
     9001 gatatggcaa caccaaatcc cctggcgccg gtaaaaggtt ccggcaccac gctctgggtt
     9061 tacaccggta agggcgatgc ttatgcaaac ccgctgtcag atgatgagtg gacgcgtctg
     9121 gcaaaaataa aggatctgac ccccggcgag atgacggcgg aatcctacga cgataactat
     9181 ctggatgatg aggatgccga ctgggtatcc accgggcagg ggcagaaatc cgccggtgac
     9241 accagtttta cgctggcctg gaagcccggc gagaaagggc agcgtgattt gattgcctgg
     9301 tttgacagca gtgagacccg ggcctacaaa atccgcttcc cgaacggtac ggtggatgtg
     9361 ttccgtggct gggtgagcgc cattggtaaa gcggtgaccg ccaaagaagt gattacccgt
     9421 acggtaaaaa tcaccaatat cggtcgtcct tcgctggcgg aggatcgggg agaaatcaca
     9481 ccggtcaccg gtattaccgt gacgccacta acggggaatg tggcaaaagg tcagagcacc
     9541 accctgacag tggccgttca gccggaaggc gcaacggata aaacattccg cgcaacgtcg
     9601 gcaaatcaga atttcgccac cattaccgtg aaagggaaca cgatcaccgt gaaaggtgtt
     9661 gcggcaggta aagcgcagat ccctgttgtc accggcaacg gtgagtttgc ggcggtggcg
     9721 gagatcaccg tcacggatgg cgctgcgggc tgaggggagg agatcaagca tgttcctgaa
     9781 aacagaacaa tttgaatata acggtgtgtc cgtcacgctg tcggagctgt ccgcgctgca
     9841 gcggtttgat tatatgaagt ttgtttcaga cgcagaacaa caggggacaa cgaagcatga
     9901 tgtcgtgcac attaaccagc gatatctgga aacggcatcc ctgcttgtgg cgatgtcgct
     9961 atggcattcc cattccctca aaggcactct ggcctctccg gagacagaga tgcagcagat
    10021 ccgccgtgaa gtgatgctgg gatggcctgc tgatgcactg aatcaggcaa cgaaccgggt
    10081 gctttatctt tcaggtatgc tggataaccg gcacgatgcc gatcctgaac aaaccgggaa
    10141 agcagaagcg actgagccgg taacatcaaa aaagcattcg aaggcgagct gaactttgtc
    10201 ctgaaactgg cgcgagagat ggggagaccc gactggcgcg ccatgcttgc cgggatgaca
    10261 tccaccgaat atgccgactg gcgacgtttt tactgcacgc attattttca ggatacccaa
    10321 ctggacgctc atttttccgg gctgatgtac gccgtactca gcctgttttt tggcgatccg
    10381 gatatgcatc cggcggattt cagtctgctt gcgccagtgt gtgaggaaga gcagacggag
    10441 atgccggacg aggaagacat gctgatgcag aaagcggcag gagttgccgg aggcgtccgg
    10501 ttctgtgggg tgggtggggg cgatatttca ccttctgcgg atgtggtgga tgtcagcgaa
    10561 gatgatgttg cattaatgat ggcttcagcg gggatttccg gaggtgtgag atatgtccca
    10621 gccagcgggt gatcttgtta ttgatttgag tctggatgcg gcccggtttg atgaacagat
    10681 ggcccgggta cgccgccatt tttccagtct ggaggcggat gccagaaaaa ccgccagtac
    10741 tgttgaacag gg
//
LOCUS       DAZGHF010000154        10708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGHF010000154 DAZGHF010000000
VERSION     DAZGHF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.155"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            56..1198
                     /gene="yghO"
                     /locus_tag="ACHK3A_004235"
     CDS             56..1198
                     /gene="yghO"
                     /locus_tag="ACHK3A_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO8742243.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     SEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHYGMIDAIDD
                     PQVFAALFGAAEAWLKSQGANKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1205..1969)
                     /gene="glcC"
                     /locus_tag="ACHK3A_004236"
     CDS             complement(1205..1969)
                     /gene="glcC"
                     /locus_tag="ACHK3A_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO8742244.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2220..3719
                     /gene="glcD"
                     /locus_tag="ACHK3A_004237"
     CDS             2220..3719
                     /gene="glcD"
                     /locus_tag="ACHK3A_004237"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO8742245.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3719..4771
                     /gene="glcE"
                     /locus_tag="ACHK3A_004238"
     CDS             3719..4771
                     /gene="glcE"
                     /locus_tag="ACHK3A_004238"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO8742246.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFSPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4782..6005
                     /gene="glcF"
                     /locus_tag="ACHK3A_004239"
     CDS             4782..6005
                     /gene="glcF"
                     /locus_tag="ACHK3A_004239"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO8742247.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVTPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGVEAILQTASGCGAFVKEYGQMLKNDALYADKARLVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASADRTS
                     VRHWIEIVEQALEKE"
     gene            6010..6414
                     /locus_tag="ACHK3A_004240"
     CDS             6010..6414
                     /locus_tag="ACHK3A_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO8742248.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6436..8607
                     /gene="glcB"
                     /locus_tag="ACHK3A_004241"
     CDS             6436..8607
                     /gene="glcB"
                     /locus_tag="ACHK3A_004241"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO8742249.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDTAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPSCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPHEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMVE
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
     gene            8963..10645
                     /gene="glcA"
                     /locus_tag="ACHK3A_004242"
     CDS             8963..10645
                     /gene="glcA"
                     /locus_tag="ACHK3A_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO8742250.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2478 a         2695 c         3030 g         2505 t
ORIGIN      
        1 ttcgttgctg ttgcaacctg tttttcaccg ttaattattt gttatccaca gagatatgga
       61 atgcgatctg ctgatgatta aaattgaaaa agtaattaat aaaaatgacc ttaaggcatt
      121 tatcgctttt ccgtcatcac tttatcccga tgatccaaac tggatacccc ctttattcat
      181 tgaacgcagc gagcatttgt ctgcgaagaa tccggggacg gaccatatca tctggcaggc
      241 gtgggtggca aaaaaagcgg ggcagatagt tgggcgtatt accgcgcaaa tcgatacctt
      301 gcaccgtgag cgttacggtg aagataccgg tcattacgga atgattgatg ccattgatga
      361 tccgcaggtt tttgccgcgt tgtttggcgc agcggaagcg tggttaaagt cacaaggtgc
      421 aaataagatc agcgggcctt tcagcctgaa tatcaatcag gaaagcggat tactgattga
      481 aggttttgac actccgccct gtgcgatgat gccacacggc aaaccgtggt atgccgcgca
      541 tattgaacaa ctgggttatc acaaaggcat tgatttactg gcgtggtgga tgcagcgaac
      601 cgatctcacg ttctctccgg cgctaaaaaa actgatggat caggtgcgca aaaaagtgac
      661 cattcgctgc atcaatcgcc agcggtttgc cgaagagatg cagatcctgc gtgagatttt
      721 caattctggc tggcagcaca actggggatt tgtgccgttt accgaacatg aattcgcgac
      781 catgggcgat caacttaaat atctggtgcc ggacgatatg atctacatcg ctgagattga
      841 ttctgcaccc tgcgcgttta ttgttggctt gccgaacatc aacgaggcga ttgccgatct
      901 gaacggatcg ctctttccct tcggctgggc aaaattgctg tggcgtttga aagtcagcgg
      961 tgtgcgaact gcgcgagtgc cgctgatggg cgtgcgggac gagtatcagt tcagccgcat
     1021 cggcccggta attgcgctgt tattgattga agccttacgc gatccgtttg cccgtcggaa
     1081 gatcgatgcg ctggagatgt catggatcct cgaaaccaat acaggcatga ataacatgct
     1141 ggaacgcatt ggggcggagc cgtacaagcg ttaccgattg tatgaaaagc agatttgatg
     1201 gcagtcaccc ctgcttcatc tccagcggca ccgccgagcg aatcaaatgg tggccttcca
     1261 gctcgatatc gtggagattc tttttcacgg tccgcacatg atcacgtgct gcgcgctggg
     1321 cgacgtgcgg cagccgctgc aacaccgcgt tgtagatccg cgcatgctgg cgatcgatct
     1381 gctttttttg ctgtggtcga tggtagagat tatttacgct ggcaaacact gaattaaaca
     1441 tcagatcggt caatgattgc agcgtaaaca ccagcacctg attgtgagaa gcctgacaaa
     1501 tggcgagatg gaaagcgtga tccaactgcg catgttcgat cagcgaaatc tctttgttgt
     1561 tctcactggc ggcgagcatt ttttcataac agcgggttat cacaacaaaa tcagcctggg
     1621 ttcccagcgt tgccgccagc cttgccgatt cgccctccag taatgcgcga acgtcgagca
     1681 gatcgtacag cgttcgcggc tgcgtactga acagatggat cagcgggctg gcgtcctgca
     1741 cccgattaag ccgtgcgaca cgagaatcgc gaccctgcgc cgtttcaata atcccgcgcc
     1801 cgcgcagcac ggtcagccct tcacgcagtg cggagcgtga gaagccgagc ttttcacaca
     1861 gtcgacgttc cgagggaagc ggctgaccga ccttcaacac gccgtcgata attaaccgtt
     1921 cgatactctc tgcaaccact tcgcaaatag ggcgacgttc atctttcatt cccggacctc
     1981 gtgcacaggt aggaccaatt tttctgttct taactcgcaa aacacgcaca tcacgcaagt
     2041 gattatgtta catcaattta acattaagtt aaccaaggca aggtcacaga gctggaaaaa
     2101 aaatggtctg accggtagct aaagagatag acgaaaacga aaagcccgct taataactgt
     2161 tcacagaagc agcgcgcaaa aatcagctgc cacacaacac aacaaagcga agcctattca
     2221 tgagcatctt gtacgaagag cgtcttgatg gcgctttacc cgatgtcgac cgcacatcgg
     2281 tactgatggc actgcgtgag catgtccctg gacttgagat cctgcatacc gatgaggaga
     2341 tcattcctta cgagtgtgac gggttgagcg cgtatcgcac gcgtccatta ctggttgttt
     2401 tgccaaagca gatggaacag gtgacggcga ttctggctgt ctgccaccgc ctgcgtgtac
     2461 cggtggtgac ccgtggtgca ggcaccgggc tttctggtgg cgcgctgcca ctggaaaaag
     2521 gtgtgttgtt ggtgatggcg cgctttaaag agatcctcga cattaacccc gttggtcgcc
     2581 gcgcgcgcgt gcagccgggc gtgcgtaatc tggcgatctc ccaggccgtt gcgccgcata
     2641 atctctatta cgcgccggat ccttcctcac aaatcgcctg ttcaattggc ggcaatgtgg
     2701 cggaaaatgc cggtggcgtt cactgcctga aatatggtct gaccgtacat aacctgctga
     2761 aaattgaagt gcaaacgctg gacggcgagg cgctgacgct gggatcggat gcgctggatt
     2821 cgcctggttt tgacctgctg gcgctgttca ccggatcgga aggtatgctc ggcgtgacca
     2881 ccgaagtgac ggtaaaactg ctgccgaagc cgcccgtggc gcgggttctg ttagccagct
     2941 ttgactcggt agaaaaagcc ggacttgcgg ttggtgacat catcgccaat ggcattatcc
     3001 ccggcgggct ggagatgatg gataacctgt cgatccgcgc agcggaagat tttattcatg
     3061 ccggttatcc cgtcgacgcc gaagcgattt tgttatgcga gctggacggc gtggagtctg
     3121 acgtacagga agactgcgag cgggttaacg acatcttgtt gaaagcgggc gcgactgacg
     3181 tccgtctggc acaggacgaa gcagagcgcg tacgtttctg ggccggtcgc aaaaatgcgt
     3241 tcccggcggt aggacgtatc tccccggatt actactgcat ggatggcacc atcccgcgcc
     3301 gcgccctgcc tggcgtactg gaaggcattg cccgtttatc gcagcaatat gatttacgcg
     3361 ttgccaacgt ctttcatgcc ggagacggca acatgcaccc gttaatcctt ttcgatgcca
     3421 acgaacccgg tgaatttgcc cgcgcggaag agctgggcgg gaagatcctc gaactctgcg
     3481 ttgaagttgg cggcagcatc agtggggaac atggcatcgg gcgcgaaaaa atcaatcaaa
     3541 tgtgcgccca gttcaacagc gatgaaatca cgaccttcca tgcggtcaag gcggcgtttg
     3601 atcccgatgg tttgctgaac cctgggaaaa acattcccac gctacaccgc tgtgctgaat
     3661 ttggtgccat gcatgtgcat cacggtcatt tacctttccc tgaactggag cgtttctgat
     3721 gctacgcgag tgtgattaca gtcaggcgct gctggagcag gtgaatcagg cgattagcga
     3781 taaaacgccg ctggtgattc agggaagcaa tagcaaagcc tttttaggcc gccctgtcac
     3841 cgggcaaacg ctggatgtgc gttgtcatcg aggcattgtt aattacgacc cgaccgagct
     3901 ggtgataacc gcgcgtgccg gaacgccgct ggtagccatt gaagcggcgc tggagagcgc
     3961 cgggcaaatg ctcccctgcg agccgccgca ttatggtgaa gaagccacct ggggcgggat
     4021 ggtcgcctgc gggctggcgg ggccgcgtcg cccgtggagc ggttcggtcc gcgattttgt
     4081 cctcggcacg cgcatcatta ccggcactgg aaaacatctg cgttttggtg gcgaagtgat
     4141 gaaaaacgtt gccggatacg atctctcacg gttaatggcc ggaagctacg gttgtctggg
     4201 tgtgctcact gaaatctcga tgaaagtgtt accgcgacca cgcgccaccc tgagtctgcg
     4261 tcgggaaatc agcctgcaag aagccatgaa tgaaatcgcc cagtggcaac ttcagccatt
     4321 acccattagt ggcttatgtt acttcgacaa tgcgttgtgg atccgccttg agggcggcga
     4381 aggatcggta aaagcagcgc gtgaactgct ggggggcgaa gaggttgcgg gtcagttctg
     4441 gcagcaattg cgtgaacaac aactgccgtt cttctcgtta ccaggtacct tatggcgcat
     4501 ttcattaccc agcgatgcgc cgatgatgga tttacccggc gagcaactga tcgactgggg
     4561 cggggcgtta cgctggctga aatcgacagc cgatgacaat caaatccatc gcatcgcccg
     4621 caacgctggc ggtcatgcga cccgctttag tgccggagat ggtggctttt ccccgctacc
     4681 ggctccttta ttccgctatc accagcagct taaacagcag ctcgaccctt gcggcgtgtt
     4741 taaccccggt cgcatgtacg cggaactttg aggagcaggc tatgcaaacc cgattaacag
     4801 aagagatgcg gcagaacgcg cgcgcgctgg aagccgacag catcctgcgc gcctgtgttc
     4861 actgcggatt ttgtaccgca acctgcccaa cctatcagct tctgggcgat gaactggacg
     4921 ggccgcgcgg gcgcatctat ctgattaaac aggtactgga aggcaacgaa gtcacgctta
     4981 aaacacagga gcatctcgat cgctgtctca cttgccgcaa ttgtgaaacc acctgtcctt
     5041 ctggtgtgcg ctatcacaat ttgctggata tcgggcgtga tattgtcgag cagaaagtga
     5101 aacgcccact gccggagcga atgctgcgcg aaggattgcg ccaggtagtg ccgcgtccgg
     5161 cggtcttccg tgcgctgacg caggtagggc tggtgctgcg accgttttta ccggaacagg
     5221 tcagagcaaa actgcctgct gaaacggtga aagctaaacc gcgtccgccg ctgcgccata
     5281 agcgtcgggt tttaatgctg gaaggctgcg cccagcctac gctttcgccc aacaccaacg
     5341 cggcaactgc gcgagtgctg gatcgtctgg ggatcagcgt gacgccagct aacgaagcag
     5401 gctgttgtgg cgcggtggac tatcatctta atgcgcaaga gaaagggctg gcgcgggcgc
     5461 gcaataatat tgatgcctgg tggcccgcga ttgaagcagg tgtcgaggca attttgcaaa
     5521 ccgccagcgg ttgcggcgcg ttcgttaaag agtatgggca gatgctgaaa aacgatgcgt
     5581 tgtatgccga taaagcgcgt ctggtcagtg aactggcggt cgatttagtc gaacttctgc
     5641 gcgaggaacc gctggaaaaa ctggcaattc gcggcgataa aaaactggcc ttccactgcc
     5701 cgtgtaccct acaacatgcg caaaagctga acggcgaagt ggaaaaagtg ttacttcgtc
     5761 ttggatttac cttaacggac gttcccgaca gccatctgtg ctgcggttca gcgggaacat
     5821 atgcgttaac gcatcccgat ctggcacgcc agctgcggga taacaaaatg aatgcgctgg
     5881 aaagcggcaa accggaaatg atcgtcaccg ccaacattgg ttgccagacg catctggcga
     5941 gcgccgatcg tacctctgtg cgtcactgga ttgaaattgt agaacaagcc cttgaaaagg
     6001 aataacaaaa tgaaaactaa agtcattctt agccagcaaa tggcgagtgc aattattgcc
     6061 gcaggtcagg aagaggcgca gaaaaataac tggtctgttt ccattgctgt tgccgatgac
     6121 ggcggtcatc tgctggcgtt aagtcgcatg gacgattgcg cgccgattgc ggcttacatc
     6181 tcccaggaga aagcgcgtac cgccgcgctg gggcgtcgtg aaactaaggg ctatgaagag
     6241 atggtgaaca acggacgtac cgcgttcgtg actgcgccgt tactgacgtc gctggaaggt
     6301 ggcgtaccgg ttgttgtgga tgggcaaatt attggtgccg tgggcgtttc tggtttaacc
     6361 ggagcacagg atgcgcaggt cgcgaaagcg gcagcagcgg tgttggcgaa ataagcgaaa
     6421 acgaggagat aaacaatgag tcaaaccata acccagggcc gtttacgcat taacgccaat
     6481 tttaaacgtt ttgtggatga agaagtttta ccgggaacag ggttggacac tgcggcgttc
     6541 tggcgcaatt ttgatgagat cgttcacgat ctggcaccag aaaatcgtca gttgctggca
     6601 gaacgcgatc gcattcaggc ggcgcttgat gagtggcatc gcagcaatcc ggggccggta
     6661 aaagataaag cggcctataa atctttcctg cgtgaactgg gctacctggt gccgcaaccg
     6721 gagcgcgtga cggtggaaac cacgggcatt gacagcgaaa tcaccagcca ggcggggcca
     6781 cagctggtgg ttccggcaat gaacgcccgc tacgcgctga acgcggcgaa cgctcgctgg
     6841 ggctcactgt acgatgcgtt atacggcagc gacatcatcc cgcaggaagg ggcgatggtc
     6901 agcggctacg atccgcaacg cggtgagcag gttatcgcct gggttcggcg tttcctcgat
     6961 gaatcactac cgctggaaaa cggcagctat caggatgtgg tggcgtttaa ggtggtcgat
     7021 aaacaattac gcatccagtt gaaaaatggt aaagaaacca cgttacgtac cccggcgcag
     7081 tttgtcggtt accgtggcga tgccgctgcg ccgtcctgca ttttgctgaa aaataacggc
     7141 ctgcatattg agctacaaat cgatgccaac gggcggattg gcaaagacga ttcggcgcac
     7201 atcaacgatg ttatcgtcga agcggccatc agtaccattc tcgactgcga agattcggtt
     7261 gcggcggttg atgcggaaga taaaatcctg ctgtaccgca atctgctggg tctgatgcag
     7321 gggactctgc aagagaaaat ggagaaaaac ggtcggcaaa tcgtgcgtaa actgaatgac
     7381 gatcgtcagt acaccgctgc cgacggctct gaaatttctc tgcacgggcg ttcgctgctg
     7441 tttatccgca acgtgggtca tttgatgacc attcctgtga tttgggacag cgaaggcaat
     7501 gaaatcccgg aaggcattct tgatggcgtc atgactggcg cgattgccct ctatgattta
     7561 aaagtgcaga aaaactcgcg cactggcagc gtctatattg tgaaaccgaa aatgcacggt
     7621 ccgcatgaag tggcgttcgc caacaaactg tttacccgca ttgagacaat gctcggtatg
     7681 gcaccgaata ccctgaaaat gggcattatg gatgaagaac gtcggacctc gctgaacttg
     7741 cgtagctgta tcgctcaggc gcgtaaccgc gtggcgttca tcaataccgg tttcctcgac
     7801 cgtaccggcg atgaaatgca ttcggtgatg gaagctggcc cgatgctgcg taaaaaccag
     7861 atgaaatcga cgccgtggat caaagcctac gaacgtaata acgtgctttc cggtctgttc
     7921 tgtgggctgc gcggtaaagc gcaaatcggt aaaggcatgt gggcaatgcc ggacctgatg
     7981 gcagacatgt acagccagaa gggcgaccag ctgcgtgccg gggcaaacac ggcctgggtt
     8041 ccgtcgccaa ctgccgctac gctccatgcg ctgcactacc accaaaccaa cgtacagagc
     8101 gtgcaagcca acattgccca gaccgagttc aatgctgaat ttgaaccgct gctggacgat
     8161 ctgctgacta ttccggttgc tgaaaacgct aattggtcgg cgcaagagat ccaacaagag
     8221 ctggataaca acgtgcaggg gattctgggg tacgtggtgc gttgggtgga gcaggggatt
     8281 ggttgttcaa aagtgccgga tattcacaac gtggcgttga tggaagaccg cgcaacgctg
     8341 cgtatttcca gccagcatat cgccaactgg ttacgtcacg gtattctgac caaagatcag
     8401 gtgcaggcgt cgctggagaa tatggcgaaa gtggttgatc agcaaaacgc tggcgatccg
     8461 gcttatcgtc caatggtgga gaatttcgct aactcgtgtg cttttaaagc tgcctgcgat
     8521 ttgatcttcc tcggagtgaa acagccaaac ggctataccg aaccgttatt acacgcatgg
     8581 cgtttacgcg aaaaagaaaa tcattaatag cgtcaaaaaa agccccggca agatttattg
     8641 tcggggctat gatagtaact gcggtagtac atttatcccg gaaataaccg gagcgattat
     8701 caggggttaa caatattaaa tacccgtagc gcatcactag cgggattaaa acaacaatat
     8761 ggacatctta acgatactct gcgcctgcgt gtgcggaaca gaaaccctta cccgatatac
     8821 ttgttatgcc agaggtagca attgcgttag cggcgtactg ttgctgtaaa gtataacgcc
     8881 tatattttac agttcagtcc tcttttgctt attacaacct taagtatatc aagcatataa
     8941 agataataag agactgaaca atatggttac ctggacccaa atgtatatgc cgatgggagg
     9001 actggggcta tccgctctgg tcgccctgat cccaataata ttcttcttcg ttgcactcgc
     9061 ggtattacgt ctgaaaggac atgtcgctgg agcaataacc cttatattat ctattctgat
     9121 tgcgatattc gcctttaaaa tgccgattga tatggcattt gctgctgcgg gctatggctt
     9181 tatttatggt ttatggccta ttgcgtggat tattgtcgcg gcggtattcc tgtataaatt
     9241 aaccgttgcc agcggccagt tcgatattat ccgcagctcg gttatctcca tcaccgacga
     9301 tcagcgtttg caggtgttac tgattggttt ctcctttggt gcgctgctgg aaggggcggc
     9361 tggctttggc gcaccggtgg cgattaccgg tgctctactg gtgggcctgg gcttcaaacc
     9421 gctatacgcg gcggggctgt gtctgattgc caacactgcg ccggtggcgt ttggtgcgtt
     9481 gggtgtgcca attctggtcg ccggtcaggt aacgggaatc gatccgttcg acattggcgc
     9541 aatggcggga cgtcagttac cgttcctgtc ggttcttgtg ccgttctggc tggtagcaat
     9601 gatggacggc tggaaagggg tgaaagagac gtggccagcg gcgctggttg cagggggaag
     9661 cttcgctgtc actcagttct ttacctctaa ctatattggt ccggaactgc cggatattac
     9721 ttcggcgctg gtgagtatcg tctcactttc tttatttctt aaagtctggc ggccaaaaaa
     9781 taccgaaacg gcaatcagta tgggacaatc cgcaggtgcg atggtggtga ataaaccgtc
     9841 ttctggcggt cccgtgcctt cagaatatcg tctggggcaa atcattcgtg cgtggtcacc
     9901 gtttttaatc ttaacggtgt tggtcaccat ctggaccatg aagccgttta aagcgttatt
     9961 tgctccgggc ggcgcgtttt attcgctggt gattaatttc cagatccctc atttgcatca
    10021 acaagtgttg aaagcggccc ccattgtcgc ccagccgacg ccaatggacg cggtgtttaa
    10081 attcgacccc ctctctgctg gtggcaccgc tatttttatt gcggcgatta tctcgatctt
    10141 catcctcggc gtggggatca agaaaggtat tggtgtcttt gccgaaacgc taattagtct
    10201 gaagtggccg atactgtcga ttggcatggt gctggcattt gccttcgtga ccaactattc
    10261 cggcatgtcc accacgctgg cgctggtact ggctggtaca ggcgtaatgt tcccgttttt
    10321 ctcaccgttt ctcggctggc tgggcgtatt ccttaccggc tcggacacct cctctaacgc
    10381 cctgtttggt tcactgcaat cgaccacggc gcagcaaatc aacgtctctg acaccctgct
    10441 ggtggcagca aataccagcg gcggcgtaac tggcaagatg atctccccgc aatctatcgc
    10501 cgtggcctgc gccgcgacgg gcatggtggg ccgagaatct gaactgttcc gctacaccgt
    10561 gaagcacagt ctgatttttg ccagcgttat cggcattatc acgctgttgc aggcgtatgt
    10621 gtttaccggg atgttagttt cgtaagtcaa taatgccgga tgatcgttaa tatcatccgg
    10681 ctacatttac attattctgt cttattaa
//
LOCUS       DAZGHF010000155        10448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGHF010000155 DAZGHF010000000
VERSION     DAZGHF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.156"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1609)
                     /locus_tag="ACHK3A_004243"
     CDS             complement(<1..1609)
                     /locus_tag="ACHK3A_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742251.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGVAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPGV
                     ATVQDIFAGGSRGADEVIDTLPDGKNVVTDTVGKGLKATGKAVSDGAKASDEWLTEQM
                     SPGAVRALNTPITEGYDDSAVLLAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTAG
                     LEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNA
                     DYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATAPQLLAVN
                     AMAAKLGDAQLLNLAMRGTAKTVKSGIVRNATAQGAINAAQGGYSRYQENTALRETAG
                     MDVSPWEGVADATIEGAALGAAMGAPFGAVAGYRGRRQAAEETAMRDAETVQQDDAAP
                     QPESVDPVAQHRESMQGMNREQLLEQYADADMATEGDASATHRREAASQLLNELDEQA
                     KRQAVMNELKAKPRSELLEEYRRLSQKE"
     gene            complement(1695..2957)
                     /locus_tag="ACHK3A_004244"
     CDS             complement(1695..2957)
                     /locus_tag="ACHK3A_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742252.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLAKLQEVASNVAADAIKNGRDAQAEVDEFYAANGLQ
                     PHQQKVQQRKTQQLLQKWQTWAAGDPVKQEFVKEAAANAPALLEPGREKEVEIYYQNH
                     LRDKKAEHEKALDYSASAAAEKIKGWMK"
     gene            complement(2968..3219)
                     /locus_tag="ACHK3A_004245"
     CDS             complement(2968..3219)
                     /locus_tag="ACHK3A_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO8742253.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            complement(3229..3675)
                     /locus_tag="ACHK3A_004246"
     CDS             complement(3229..3675)
                     /locus_tag="ACHK3A_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742254.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAV"
     gene            complement(3678..4334)
                     /locus_tag="ACHK3A_004247"
     CDS             complement(3678..4334)
                     /locus_tag="ACHK3A_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742255.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(4428..4829)
                     /locus_tag="ACHK3A_004248"
     CDS             complement(4428..4829)
                     /locus_tag="ACHK3A_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742256.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSDDGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            complement(4886..5026)
                     /locus_tag="ACHK3A_004249"
     CDS             complement(4886..5026)
                     /locus_tag="ACHK3A_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8742257.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(5259..5993)
                     /locus_tag="ACHK3A_004250"
     CDS             complement(5259..5993)
                     /locus_tag="ACHK3A_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO8742258.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGEHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFINRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGIGVAKVTADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            complement(6084..6701)
                     /locus_tag="ACHK3A_004251"
     CDS             complement(6084..6701)
                     /locus_tag="ACHK3A_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742259.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            complement(6707..6985)
                     /locus_tag="ACHK3A_004252"
     CDS             complement(6707..6985)
                     /locus_tag="ACHK3A_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742260.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(7000..8268)
                     /locus_tag="ACHK3A_004253"
     CDS             complement(7000..8268)
                     /locus_tag="ACHK3A_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO8742261.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(8265..9890)
                     /locus_tag="ACHK3A_004254"
     CDS             complement(8265..9890)
                     /locus_tag="ACHK3A_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742262.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDAHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(10252..>10448)
                     /locus_tag="ACHK3A_004255"
     CDS             complement(10252..>10448)
                     /locus_tag="ACHK3A_004255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742263.1"
                     /translation="WLGTGITDKKMGDHHLPHELSQLYTCEVFTMRINTVTESAGLDV
                     MEIMFPAHPVDMDNFSDFRK"
BASE COUNT         2494 a         3006 c         2235 g         2713 t
ORIGIN      
        1 cctctttctg gctgagtctg cggtattcct caagcagttc agaacgcggc ttcgccttca
       61 gctcattcat cacagcctgt cgcttcgcct gttcgtccag ttcattcaac agctggctgg
      121 cggcttcccg gcgatgagtt gcggatgcgt caccctctgt cgccatatcc gcatcagcat
      181 actgctccag aagctgctcg cgattcatcc cctgcatgga ttcacgatgc tgcgccaccg
      241 gatcaacaga ttctggttgc ggggctgcgt cgtcctgctg cacggtttca gcatcacgca
      301 tggcggtttc ttctgcggcc tgacgtctgc cacgatatcc ggcaactgca ccgaatggag
      361 cccccatcgc agcaccaagg gcagcacctt cgatcgttgc gtcagccacg ccctcccatg
      421 gcgatacatc cattccggcg gtctcacgca atgccgtgtt ttcctgatag cgtgaatagc
      481 cgccctgcgc cgcattaatc gccccctgtg cagtggcatt tctgacaatg ccacttttaa
      541 cggtcttcgc tgtgccccgc atcgccagat taagcaactg agcatcgccc agttttgccg
      601 ccatggcatt cacagccagc aactgaggag cggttgccag ttgcgcacgc acctcatcag
      661 ccacacgctc ttttgccaga tccattttct ggcgatcagt aagctgtgcg tgctgcgggt
      721 cggcgtcaat ggacaaaaac gtctgctgaa atttcggtga ctgcgccagc tcagagtaat
      781 ccgcattaag aacagcatct gctgccgcca ttgcactctg cccctgtgca ctggctgtgg
      841 aatgggtgat caggcccgcc tggaataaat ccggcatttt tttatcgaca gcttctgctg
      901 ccagtgccgt ggctctttcc ggctgcatcc ctgccgcgat gtattttttc tccagcccgg
      961 cggtcagcat ttttcgcagt gtgacatcac ccacctttct agccacaccg ccagcaacca
     1021 tatcaggtac aagcgcacca atcaggttta cacccttcgc cagcaagacc gcagaatcat
     1081 catagccttc tgttatcggc gtattcagcg cacgaacagc acctggcgac atctgttctg
     1141 tcagccattc atccgaggct ttggcaccat cactgacagc cttaccggtt gctttcagac
     1201 ctttaccgac agtatcagtg accacgtttt ttccatcagg cagggtatcg atgacctcat
     1261 cagcccccct gctgccaccg gcaaaaatat cctgaacagt tgcgacaccc ggtagcccca
     1321 tacggctgaa ctcatttaaa atacgcgccc ctgtttttac cgggctctga atcatcgcat
     1381 caccgagtcc acgggccatt tcccctgttc cccggacgga ctgggcaaca cctttaccca
     1441 ttgttggcaa tacatcaccg aggctgaacg atgtgctgtg atcaatggca ttctgcgggt
     1501 tgctgaaaaa atcctgccag taattttctc ccggctgctt aatgttcagg ctgctgcggt
     1561 tctggttacc caattgcgct tcagggcgct gctcatctga aaaagccata cagactccag
     1621 taaaaaaaag cccgacacga tggtcgggcg taagtgaacg atattaacgg ggagcgcgtt
     1681 actgccgcat atctttactt catccagcct ttgatcttct cggcagctgc ggaagcgcta
     1741 taatccagcg ccttttcatg ctctgccttt ttatcccgga ggtgattctg gtaataaatt
     1801 tccacctcct tttcacgacc aggttccagc aaagcaggcg cattggcggc agcctctttt
     1861 acaaactcct gtttaaccgg atcgcccgcc gcccacgtct gccacttctg taaaagctgt
     1921 tgcgttttcc gttgctgaac tttctgttga tgcggctgta aaccattcgc agcataaaat
     1981 tcatccacct cagcctgagc atcacggcca ttcttgatcg catccgccgc tacgttgctg
     2041 gctacttcct gaagtttcgc cagagaaagt ccctgctgct tcggcataaa ataaccataa
     2101 tttttcgata tctctgcaaa tttgctgcga tccctgacct gagcgatagc cttatcaacg
     2161 ggtatcgcca gcacggtttg atcatcaggg tgcgcactgc cgtattctgt tacaggttta
     2221 tgcgcggtgg agccatcgct gtatgtgaga tcaaggccaa taagtacgta cccttcctgc
     2281 tgcgccggta cgatactgcc aatcctggcc tcttttatcg ttttctcccc ggttgaatca
     2341 ggcatgccaa tacgctgttg tagttccggg gcaaacacgc cggaaagcac atcgagattt
     2401 tccggggtat tcagcgaagc aatcgccctg tccggcttat catcaaagat tttctgcagg
     2461 ttgttcacgg cctgacccgc tttcgcagca tagcctaccg ttgacatcat cttaagcgga
     2521 ttggcgtccg ataatttacc gaacagatga gaagccgcat catggtcacc cgcatcaatc
     2581 gcttttccca gcgccgccat cacaggctga tcgcgcgcca gcatatcgtt atactcaagc
     2641 cgtcgctggt tgtatttctg caactggagt cgttgctgtt ccatccccag cgacgcattc
     2701 cggtaattct ggttggcgtt aaactccctt tcctgcagtg tgtaattgcg gtcatcaact
     2761 ttggctctgt aatcaaagtt ccgctgatca acgtctttat tatgttcaaa ctgagactgc
     2821 gcaaattcaa aatcgcgctc gttatttttc tgttgctgag caagctgtgc ttctcgcaaa
     2881 ccaagctcct tacgacgggt cattgcctgg tcaacagtgc tgaatccggc aagtaacccc
     2941 tgtgcaaatc cgctcattca ccactcctta aaacaaagaa ccagcaatgc cgccaattac
     3001 tgcaccagca acagcgccaa caggaccacc aacggatgcg ccaatagccg cccccgtacc
     3061 aatgcccgta ccgatattct gttttttctg agctttctgt tgagcagcca gttgtttatt
     3121 tgatgcctca atctctgtgc gtcgtctgtc tgcgtcactt attccctgta atgcctcacg
     3181 ccgcgactga tttgcaatat ccagtaaacc gtatcccata ttgccccctt acactgccac
     3241 cagttgtccg ccaacactca gtttctgtct tgccggtgca gaagcccccg tcagaatatt
     3301 catctggcga tcctgttcgg cttcacggat accatttttc gcgccagcaa ttgccagggc
     3361 attacgtaac cccagcgtat tactgtcggg attatccggg cggcttaccc cgtatctcgc
     3421 catctggtta tcctgcgcca tctgcgctgt acggagactg gaagtggcaa ggccgcccac
     3481 ccgggcaagc tgtgcattca tcaggctgtt gttctcgcca aggtcagcca gccttgccac
     3541 gcggggcaaa tatctggttc gccagtcgtc gtattgttgg cgtgtcagcg ctgctgacgt
     3601 ctgccagtca ccctgtggac gggcagctcc ggtataaccg tatctcgcaa gtgtttcgta
     3661 tttaccgtac tccataatca cagtctccag ttctgagcct gatgctgaat ggcattagcg
     3721 ccggtgccag gtgttttagc accgccactt cctccgctac cgccagcctt atgcatcgca
     3781 tatgcacctg ccgcacccaa accagcgcca acaagagagg ctcgcccctg ctgtttcgta
     3841 aacgcagcct gtgcgtccga tttagctttt gccagactgc tgtctgccag cgagttaaaa
     3901 ctctgtaacg catccgcctt ctgaccagaa ccgagagctg caacatcctg tagcccggca
     3961 acatacttat ctgcctgcga tacctgcccc cgtgtggttg tgtcaatctg cccggttacc
     4021 tgatcactct ggttcgcatt cattaccgcg ttaaaacgac cactggacgg gtcaacaccg
     4081 gactgagcaa gattacccgc cagctccttc cgtgcttcgc caaactgttt ctgatacccc
     4141 agatttgtgg tgccagcaat attgtcgtac tgctgctcac tgttcagatc atcgaccttt
     4201 tccatgaagt tatcttcagc cgggcggagg attttttgat aatcctgcca ccctttccag
     4261 gccacttctt cctgtgctat ttgcgctgct gttggtttta ctttggtatc accaccgcct
     4321 ttacttccac ccataatggc cccctggata acaaaaaacc ctgccggagc agggtcagaa
     4381 tgtgaattac aatgttggtt taaacacaat gatgaaaact gtagcagtca gaccgctatc
     4441 ctgaacacca tgaagccatc ctcatcatcc ggcattcgct caaagcccag acgttttccc
     4501 agccggataa atccccgcct tgccgtatga aattcagccc agcgtccgcc agccagacgg
     4561 gttaatgtct tcacctccgg cagataacgc tcaacgctgt tactccccgt acacacgccc
     4621 agcaacacca gaacataagg gataccatcg tcactgagca cagaacgcag caccagaaag
     4681 ccatcaggag cctcaaaaca aaacgcctgc tttttaaggc aggcgtcttt aacttcattc
     4741 ataaattcag ggttgcggga atttctcacc acacgctgca tataccggag aattttatcg
     4801 ttcattctct cacctgaaac gggtgccata tcggctgaac ctcagcaacc agttgacggg
     4861 gactttcgtc cccgccgcgg ttttcctact gcttacactg taagaacgcc gcaaactccg
     4921 ctccccacaa attcagccgg aactcacaca acgagccgtg taacatccag atggtgaaga
     4981 taaccgtcat acaaattgtg acggtgataa gcgatttttg cgacatagcg cttgactcct
     5041 tttacagaga ggcgctaacc ttctacttgc ttaaggtacg aagtgttagg gcctcgggtt
     5101 aacgttaagt tgactcgggg cctttccaca tcaggccttc aggttcaccc tccagccatc
     5161 agccgaaagg cacccacgca taatttacga tttttgccct gtacgggcaa taaaaaaccc
     5221 gccatcacag cgggtagtaa atggttaccg gatacaggtc agaatttcag gccaatgcca
     5281 gcagtaacgc cactcgtgcg ccagtcgcca gagccggaac cttcgtaagc cacatccagt
     5341 gtaacactct cattcaggtt aaactgtgca cctgcagccc acgcaaggga ggtttttttc
     5401 gtgctgttgc tttcagaaaa tccgccacta ctgttaatat tgtccttaat tttcaggtca
     5461 gcggtaactt tagccacgcc tatccctgcc atcgcatata aactcatgta ctgattaacc
     5521 cgccatgaag gccccgctaa cagactccag taattcgccc tgatatccgt tctggcagaa
     5581 gctgccggat tcttaatctt cctggtatga tcggctgact gcacatcaat aaatgtctgt
     5641 gagttagtga gagaacgcgt ccatgtaaaa gaggtgataa cgccaaaatc atccgttatc
     5701 tcgtagcgat acctgatatt aatgccctgt ggatttttat ccttgccttc atatccactg
     5761 acccgaccat ctgtatattc gcccaatgaa gaaaagtagt ttctgttgat gaaatgactg
     5821 aaagtctccc tgttatgagc agtcgcatcc tttacaaaat ccttcagtcc cggaaactga
     5881 aagtgcgcat accccagaga aattgtatgc tctccttctg ctgcatgtgc agataaattt
     5941 acacaggcaa tcccggagat tgcacacaca accagtgttg ctatactctt catttaagat
     6001 atcctgtatt cagataacac gctaaacttt ttaataatac atctttttag acattttaaa
     6061 tgaatattaa agactcgctt caactaatca tatactgatg tattaataac caggacctta
     6121 tctatatacg gctttcgaat atcccatgtt gcaccaccgg aataatgctc acaggaatat
     6181 attttatttc ctgtagcacc agtggatgtg gtataaatcg gtcgatcata aggagttctt
     6241 ttccagttat aatacccgac cagtgcaggc atgattgcgc atggatatcc aaggtccttt
     6301 tcaaatttga tatcaacagg tattaacttc gcgtcaagca gcatcatttc gccatgataa
     6361 atcatctcac cgtccgggtt atacatggcg ataccatact cagaaggtgg agtaaccata
     6421 ttcgcgaaag cataaaccgt cgtaacacca gggttcgttc ccctgacaat tacatgaagc
     6481 cttagtgcat gatatccatc aatctgttca tgcgtgtaca tgacatcggc cttcttctct
     6541 gttctgataa agaaaaaaca acttttgcct gacgggattg atgttttaaa aaaagacttt
     6601 tcagtcgccg gtatggtgcc tttgttgatc agacactgcg gcgtaaaacc tggacttatc
     6661 catacgctgc catccggctt cataatgctc aaaccataca tgacacttat ccccagaatg
     6721 tataaatata agatcccata ccctgctcaa gattcgacca cgtcaccgta ttgccattaa
     6781 tggttatctt cggtactttc cgatccgcaa atacattatt ccagggaaat aaacaacata
     6841 cagcctgcaa tgatttcccg tcgggtttat tcgtgtacgt ctttgagcca gactccgctg
     6901 taaatctgtc caggaaaaat accggagtca gcacgcccgt aacattaacg ttatttctgt
     6961 tataaatggc aaaaccgtat tccaatactc acctcctgat cagcgtaatc tgcctatgcg
     7021 aacagccagt cgtccattct gatcataaac ctcaatttta tcattgcgga tcaccagtcc
     7081 tacattctga ttagagtaac gaattgtcag ttgcccttgt gacgtaacac tgaaaaggcc
     7141 tccaatattc aggttaccct gagaatcaac ctgaaagttt ccgttctgaa taacggcact
     7201 ccggataact ggcgaagtga tacttacccc ggcttttacc tcatccgcca caaccttccg
     7261 cgacaccagt gtttcaatca ccgcgtcata aatcatcgct ttcgggatca caaccttgcc
     7321 acctgatacc gcaaacggat aggcggtgtt atccgggttg ttcgggtcaa agacaaacaa
     7381 ctgcgacgca gaaattgcaa cctgacttac aggcctgcct tcactgtctt ttccggcgac
     7441 aatcccgatc cccgcagtga taccatcaac tcccgctttt tttgaccaca ttgccagaaa
     7501 cgcctcaccg ccttctttat ccagtttagt gatgcgcttg tcgacctcat taagcgattc
     7561 actggttgac gaatccagtg tgctaatgcg ggtttcaata ccaccaatcg ttcttgtcgt
     7621 ttcttccctg agagtcccca caacttcggt tgtcttaatt gcagcatcct ttacagcctg
     7681 cccctgcgcg ttttttattt ctttacgcag ctcggacaca accggcgact ttgcagcctc
     7741 atcgcggatc tggtcaatga tggccttcac gccgatctgt gtttgtgcct gagtgccttt
     7801 ttcagcattc catggacctt tcactcctgc cgcgttaaca aaacgtatcc agtaaaatcc
     7861 cgaccagcca gggtcaaccg gatcgccgta aacctgcccc ggcgtcgtgg caaccagcac
     7921 tgcatcagca aggtcatcct ccgtaccccg ccagatttca gtcagtgaat gtccgcgata
     7981 attaggcata tcccattcaa gaagaaccga gccaaatcct cctgtcgcct taaaattcag
     8041 cggttttgtg ggaaaatcaa cagtcattaa agtactgtca atctcaatac ccggattcag
     8101 tgcatatgag gcaccacccg atgttcgacg ccgggcgagt ttaagaccaa ccagttcctc
     8161 acgggtcaca aatgcgtggc gtccgtcacc acgctgcccg gtgccaattt ccatgttctc
     8221 cacaactgtg gataaatcct tccccgcacg ccacggtttt ctggtcatac cggcatctcc
     8281 gacatcgatg tactcagggt tattcgttcc acctgcccga atccggatac catcacctgc
     8341 cagttttgcc cggttgctgc cggaagtctc accacacttc ccttaaacgt acccggcgca
     8401 aaatgaatca caggaacatc atcagccata atggtgatcc ccacccgctc aggcgccgga
     8461 gatttcaccc tgatacagga aaaagaggtt ctttcaggta atgaaaaaat ttttgaatgc
     8521 caccttatcg tggagggcag agagcccccg gcaagcactg acattttgtc tcctgtcacc
     8581 acacgcatca tatctttcgc gagatcaacc catgcgcagt caaacggtgt actgagataa
     8641 cggatatcca tgttcaccgg actgaataca aacacatcct gcttaccatc cggtttcgtg
     8701 taacaggcaa tgtattcacc acgccaggaa taagccacaa tggacgccgg gttaaactga
     8761 ctctgccact gttcaggtga aataatcttt tccgttgcca gtgctgtatt accgtttaca
     8821 tcaacagata ccagaccgtt tgtcccggca tagagtacga atccctccat cgccaccata
     8881 cttcttcggc tcaggcatgc ctgcatggaa ggaattctgg agccagaaat tgtggaaggc
     8941 gatacaccac tgaacagata aggctccccc tttgtcgcca ccaccagtga cgttcccagc
     9001 ggacagatag ccacaatatc ttctgccgtc gtgtgacgat tcacttccgg ccatgcatac
     9061 ggcagatacg cttccgaaaa catcacttca ttaccggcaa acccggcggc aataccatta
     9121 gccatcaggc aaaggcctgt catattctct ggcggcggca ggtaatccca tgtagccaga
     9181 gaaggtccaa ggtttttccc cggtattttg tccgtgtaac tgagcacgga tgcatccagt
     9241 tcagccacaa gtaaaaaatc cgcttctcct ccacctgatg cagagcgata aatccggcgg
     9301 cgtttaatac tggcattctg caatggcacc ggagacagcg tcagctgtac cgcagtcccc
     9361 ggagtacgga gtgttacctc cagagacgcc ggacctggcg gaccttcttc accataatct
     9421 gagacaaagg tttccgtata aaaccgggtt tcgtcatcat tcgggttatc gtcagaaaca
     9481 tcaccgccct gctgaacagt acagacaggt gctgttgtcg gcgcgggaat ccccagacga
     9541 tacgatgatg tcgggtgatt cccgtcccct tttgtggcaa tggtcgcatc cgtcacttta
     9601 ggaaaacgcc cgtcagtgta gtaaatacgc ccgtgggcgt cctgagcgac cggactgcgg
     9661 atcacatcca ccacatccgt ccatgcaaac cagaaatcgt cacggtaatg aaaaatggtt
     9721 ttcggcttaa tcgcaaatgt tttctcagcc tctgacatct ggtgttcagg cgtgatcact
     9781 ccatagcgaa aatgacagtt ttctgccagt acagcagaat gatctggcag catagatgca
     9841 ataacgcctg gcatcatccc gcgcatagtt gttatatcga tataaggcat ggttgtctgg
     9901 tatccgaaaa tttgaatggc aggtgagtca ctgaattgaa gttttaaagg cggcatacaa
     9961 ttgcgcctgt gcagaataca gatttccaga aatctgaaaa attactctta tcagagaaga
    10021 gcgtcagaaa cacgatcggc atcactgttt ctgcaaactt ccctgtggca gtctttattc
    10081 gccatataac atagcactga atgtcttcat tgatttttcc attgtagtta ctccttcctt
    10141 gtaccggggg ttggtcatgc ccccgttttt tattacccct gttcccttaa ttgtactgcc
    10201 aggtgccaca acacacatca actgtggctg gtatggaaac accaaaaact ctcacttccg
    10261 gaaatctgaa aaattatcca tatcaacagg atgcgccgga aacatgattt ccatcacatc
    10321 caggcctgca ctttccgtga cagtatttat tcgcatagta aatacttcac aagtgtataa
    10381 ttgactcagt tcatggggga ggtgatggtc ccccatcttt ttgtctgtta ttcctgtgcc
    10441 cagccaac
//
LOCUS       DAZGHF010000156        10052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGHF010000156 DAZGHF010000000
VERSION     DAZGHF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.157"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(209..1582)
                     /locus_tag="ACHK3A_004256"
                     /pseudo
     CDS             complement(209..1582)
                     /locus_tag="ACHK3A_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1781..2827)
                     /gene="fbpC"
                     /locus_tag="ACHK3A_004257"
     CDS             complement(1781..2827)
                     /gene="fbpC"
                     /locus_tag="ACHK3A_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO8742264.1"
                     /translation="MSQKNFVELRNVTKRFGSNRVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKDALTMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTAGVR
                     PEAITLNNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(2839..4917)
                     /locus_tag="ACHK3A_004258"
     CDS             complement(2839..4917)
                     /locus_tag="ACHK3A_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8742265.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYGWSHFNISWLWYLLPSLLLVRPFREATREQRSRHYFDASWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMNVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYITEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCVQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILTVWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPLYLAGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(4986..6017)
                     /locus_tag="ACHK3A_004259"
     CDS             complement(4986..6017)
                     /locus_tag="ACHK3A_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8742266.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(6014..7330)
                     /gene="uhpC"
                     /locus_tag="ACHK3A_004260"
     CDS             complement(6014..7330)
                     /gene="uhpC"
                     /locus_tag="ACHK3A_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO8742267.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLNANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWSGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(7403..8944)
                     /locus_tag="ACHK3A_004261"
     CDS             complement(7403..8944)
                     /locus_tag="ACHK3A_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO8742268.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALLILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPAILAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(8944..9573)
                     /locus_tag="ACHK3A_004262"
     CDS             complement(8944..9573)
                     /locus_tag="ACHK3A_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO8742269.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWPALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            complement(9849..>10052)
                     /locus_tag="ACHK3A_004263"
     CDS             complement(9849..>10052)
                     /locus_tag="ACHK3A_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8742270.1"
                     /translation="ATGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAL
                     YIDGRREMMQWYADYIDSLSEQT"
BASE COUNT         2497 a         2714 c         2611 g         2230 t
ORIGIN      
        1 ctgaccggat tacagctatt tgatgagttt gagtacgata ttgatattgg tttcagttat
       61 tgatttattt atgtcataac ttttctgaaa gatagctggt aattgccttg agaaagtttt
      121 tatatgttat tctgtaaaac aggataacag aaaataatta tgtctgaaat aagtagatga
      181 tgtcccctct gagctggctg tctcttgatc agatatcctg atcaagagac ttcatcacca
      241 ggtaaccctc aaccatatcc tgaagtctga accagtcatc ccacataact acccaaccgg
      301 ggcggcctgt gcgtttgctg tcatgtcatc gccccagttt agccagtttc agactggctc
      361 atttcaggat cggtgcctgc gccggaagcg gctctccttc cagcttaacc cacagcagtt
      421 tccactctgt cggcgtcagt attttctcac aactgtcatt ttgtgtttct tcactgacac
      481 ctccctgccg caggcccggc acccgtaccg cgataaacgc cttgataacc accatgcgct
      541 caaggttatc ccgggtctga attcgcagcg attccacaca tgtaccacca cttttccacg
      601 ccttgtggta ttcctctatc agccagcgtc gctcgtaatg gctgacgata cgtcgcgcat
      661 cggcggcact cgccactttt tctgacgtca gcagatgcca gcaggcactg tcctctgcct
      721 gctcccggca acagacatac gtgagcgaga gcgcctggcc gctgttgtcg ggatttttta
      781 tgctgacttc gctgtaactg atgaacatcc gggcctggcg ggctgcccgc ccgccttttt
      841 gcatcacatt cagcgtgtgg cttcccgcgg ttgccaggac ttccggcagt tcgaagagct
      901 tgccgggtgc ttcttccagc cggctattct gtgcagcacg caccacgaag cgctgtccgt
      961 ggctgacttt ataatgcagg taatgccaga tatccgcttc ccggtcacag acagtgatta
     1021 cccgtttctg tatctccccc agccgttcgg ccatgcgctc cgaagcctgc tgccagcggt
     1081 aactttcttt ttcttcacag ggacgttctt ttcgctggtg cttaacaccg taggtgtccg
     1141 tggcccgact ccagctctgc tgttcgataa gagcgactgg cagggcgctg tcgggggcgt
     1201 atatcaggac agagtgagcc agcagcccgc gcgtcttcgg gttagtggtg gtattcccca
     1261 ggtcatcaga tgccgtactg tggctgaagt taatggtgga ggtgtcttcc agtgcgggga
     1321 gcagcggatg tgcctcacat gcccttgcag tggcggtaaa tccggctccg gaaatggctt
     1381 ccggggacac tgacgggtta cgcatcaggc ggtacgcacc ttcaacctga gcagtggagt
     1441 gggattattt cacaatagaa agacctgcat gctgagcgag agaagaggcc agtgacacaa
     1501 ggcgtcgtgt acgacgcgga tcaccgagac gggcatgtcc agactgctcg ttagcccatg
     1561 aataacaatc agaaagtacc ataacagacc cgaagaaaat gaaatataag agaagatcaa
     1621 cgggtgaaga aaaagttcaa aaaatcgctg ccggggagga aggaaactac cggatggaaa
     1681 gagtcccccc taaagcagac tgacagacat cacaaatccc caggtggtga tttgtgtata
     1741 agagacagct gagctggagg ggaccgctta tgcgctattt ttatgccgca tccgctaaaa
     1801 caaacatccc gtaagggtgg atttcgaggt aatagtgctc accaatatcg ggctgtaaac
     1861 gggtggcgtt gacttgcaat aaaatctcct gaccatgcca ttctacggtc acttcgtact
     1921 gcggccccat gtaagcgaca tggcgaataa cacaacgctg actctcttcg ccgtgattgt
     1981 ttaaggtgat ggcttccggg cgaacgccgg cggtgccgct accttgcgcc gggaaatgcg
     2041 ctgcgcgcgg caggcgataa ccgtagatat cgacatactc ttcgctaaag ttcgccggga
     2101 acaggttggc gtcgcccata aaacttgcca taaatcggga ggctggctga cgatagagat
     2161 cctgcggtga gccaatctgc atgatgtgac ctttattcat caccagcaca gtgtcggaca
     2221 ccgcgaaagc ttcactttga tcgtgagtca cgtagagtga cgtgatatta aactgctttt
     2281 gcagctcgcg gatcttatcg cgcatgctgc ggcgcaggtt ggcatcgagg ttacttaacg
     2341 gctcatcaaa cagcagcacc tttggcttga ggatcaacgc acgggccaga gccacacgct
     2401 gttgctgacc accggaaatc tggtcgacat agcggtcttc gaatccttcc agatccacca
     2461 ttgtcagcgc atctttcacc cgctgcttca cttcactacg cgacacgcca agcattttca
     2521 gcccatagcc aacgttttcg cccagcgaca tatgcgggaa cagagcgtat gactgaaaca
     2581 ccatgcagat atcgcgttgc tgaatggaac gatgcgtgac atcttcgcca tctataaaaa
     2641 tttgcccttc gctcggtttt tccagcccgg caaccagacg cagaatggtg gttttgccgc
     2701 atccggaagg accgagcagc gtcaccattt gcccttgtgg gatggtgaga ttgatattgt
     2761 cgataaccct attactgccg aatcgtttag tgacgttgcg cagttccaca aaatttttct
     2821 gactcatagt gcgctccatt acgcctggtt tttggcttta gaacgggaaa tacgtgcttc
     2881 accgatcagc cagtcaaaga ggaaaataat cgccagcatc accacgatca gaatcgaacc
     2941 gtaggcgatg gctacgccat attcgccgtc ttctacgcgg ttaaggatgt aggcggttgc
     3001 cacgcgtgtg tccggtgtga cgagaaagac gatggcgctg actgtagtga tggcccgcac
     3061 aaagctgtaa atcagggccg agagaatggc cgggcgtaac agcggcagta aaatgtgcgt
     3121 aatggttcgc agggaacccg cacgcaggct aagtgaggct tcatccagcg atttgtcgat
     3181 ctgccccaga cctgcaatcc cggcgcgaat acccaccgga acgttacgca tcaccatcga
     3241 gataataaca attgctgccg ttccggcgag atagagtggg gcgctattaa aggcaagaat
     3301 atacgagaca cccgccactg tacccggtac ggcaaagcac agcatggtgg tgaactcgat
     3361 ggtctttttg cctttgaatt gttgacgcac caccacccag gcgatgagca ggccaaagat
     3421 agcggtaatg ggggctgcaa tcccggcgta gagcagggta tccagcagtg aaggccacgc
     3481 gccgtcgctc atcccctgac caaataactt gataaagtta tccagcgtca gggtgtagtc
     3541 aacgccccag ttcaccgtga agctgccgta gaaaatgctg ccgtagagca gggcgttaaa
     3601 ggcaatccat accgtaagta tggcgaccac gctccacacc agggtgaccg gtagcggctg
     3661 gacgtcgcca cggtaggatt tgccggaaac cgtgacgtaa gagcgtttac caatccacat
     3721 atactggacg cagaacacca gcagcgagaa cagcagcagg aaagcaccaa gcgtgctggc
     3781 ggcctgataa tccagctgcg agccggtaat gtagaagtaa atttgcgtcg ccagtacgtc
     3841 gaagttgccg ccaagcacca gcgggttact gaaatcagcc agtgactgca ccacgacgat
     3901 taaaaaggca ttggcgagcg cgggctttag caatggcaca aacacgccgt taaaggtttg
     3961 ccagcggctg gcgcgcaggg tataggaggc ttcttccagc gagggatgaa tggttttaat
     4021 ggccccgtcg aggatcataa aagccattgg cgtgaatgcc agaacctgcg ccagccagat
     4081 cccggtaaaa ccatacagcc agttggtgtt ggtcagccca aaccactcca ccataaactc
     4141 cgttatgtag ccggagcgcc ccatcatgag cgtcacgcct aagccgacca caaaaggtgg
     4201 cgtaacgatg ggcagaatgg aaaaaatgcg gccaataatg gcgctgcgtt tggcgatgcg
     4261 cgtggtataa atcgccagca ccaggccgaa gaaggtacag ccaataccca ccgctatcga
     4321 caagacgata gagttgacga tcacctgcac aatatgtgcc tgcgagagca cgttcataaa
     4381 cgccagcggg gcgaactctc ccgcgtcgtt ggtgaacatc gggataaaaa tggcgatgct
     4441 cggccagacg ataaaaatgc caatcagcgc cacaatcgca atcagtgagc cgatcacgaa
     4501 gcggtcgccg cccagccatt cgaggcgggt taaggccagc gtcataatcg cgcctaattc
     4561 aacaaacagc acgatggcgg cgtagcctaa accgcgtcca gccacggtgg cgctgatgac
     4621 gataaacgcc atacagagca atgcccagct ggcatcaaaa taatgacgac tacgctgttc
     4681 gcgtgtggcc tcacgaaacg gacgaaccag caacagactc ggtaataaat accacagcca
     4741 gctgatatta aaatgcgacc agccataagc ctcgaggatc tcatcactcg tggactccag
     4801 caggccataa tcaaggctcc agctcggcaa caggataaat gccagccagc ccagcaaaac
     4861 ccaaaggaaa atcgcatccc gctttttcac gggatggagt gcaagtgtct gtgacatagc
     4921 agttccggta tggttggccg gggcaggcgt ttgtgctgca cccggcaaat gcgcctaatg
     4981 cgggtttatt tacccatctt cacttcgctg acccatttat taatcaatgc cttacgaaca
     5041 tccgtggaac catatttatc catgtcgtag ttgattaatt tcaggtcgtc gagcttcagc
     5101 gaatttggcg acgtttcggc ggtggtattg gtcaggatct gataagactt ccctttcttc
     5161 cacgccagtt cctgagcttc ttttgataat acccagtcca caaacagttt ggcgttgtcg
     5221 aggttgcgcg cgcctttcag aatgctgacg ccgccaattt cgtagcccgt accttcacac
     5281 ggggaaatca actccagcgg cgcgccctgt tctttttcca gcgagtagtc atgcaggaag
     5341 ccaataccaa tcgccgtttc gccacgggcg gcgttacgtg ccggggctat gcctgatttg
     5401 gtgtactggg agacgttggc gttcagttgt tttagataat caaaggcctg atcttccccc
     5461 cacagctgcg caaaagtagc cagggcggtg taggcagtac cggaactttg cggatcggca
     5521 atttgaattt cgcctttata ttccggtttg gtgagatctt tccagcactt cggcaccggc
     5581 aggtttttct ctttcagacg atcggtgttt acgccaaaac cgaggatccc cacatatacg
     5641 gcagaggaca gattcccttt cacttttgcc ggatcgcgga atttctccat gatctgctca
     5701 aggttagggg atttatacgc ctgtagcagg cccatctcgc cagcctgcga ttgcgggtct
     5761 aaagtgccgc cgtaccagac gtcagcctgg gggtttttct tttcagcatc gacttttgcc
     5821 agggtgcttc cggaaccgtt gcggataaac gaggttttta catcatattt ctcgccaaac
     5881 gctttggttt cggcctcaca catttcattg gtggcgctgc aatagaccac cagccggcct
     5941 ttcgcctgcg cggcacccgt taaagtcgcc agcgcaacgg cagaagcaat cagagtagag
     6001 gtaaaggtca ttttcattgt tttatccttc gagcgtgagt ggtggtgatg cccgccatca
     6061 gtaacggcat cagcagtaaa cccataagta ctgcggcgac cgagagtaaa ctgaacattc
     6121 ccgaccagcc gtagcgttcg atgaccagcg acagtggcca gcccgcaagc gccgcgccaa
     6181 ggtaagcgaa caggccgaga aatccggtga ttgaacccgc cgcagctttg tgcccacact
     6241 ccaccgccgc aagtccaatc agcatttgtg gaccaaagac gaaaaagccc acggtaaaaa
     6301 agcacactgc cagcagagcg tagtgatgaa ccggtgccag ccacagtgcg gcgactgaca
     6361 ccatcaaccc cagcgtgaac agcaaaatca ttggcgctcg ttgcccgcta aacagcaaat
     6421 ccgatcccca tccggcaaat aacgcgccga gtaagccgcc cacttcaaac agcattaccg
     6481 tggcgttggc gttgagcagg ttgacgccgt ggctttccgt cagccagata ttgccccagt
     6541 cgttaagcgc aatccggatc acataaacca gtacataaga gacgcccagt agccagatca
     6601 gcggattttg cagcatggtg gtgcgcaaca tttgccacag ccccatcggc ggactttgtt
     6661 gttcctgacg taactccagc ggatcgtgac gccagtgacc aactgtcggt aatccctctt
     6721 cctggggcgt gcctttgagt tgtagcgtta accagatccc taatgccatg ctgattatcc
     6781 ccggtgtcag catcgcggcc tgccagcccc accagtgggc ggcaaaggcg ctaatcagcg
     6841 gaataatcgc tccgccaatg ttgatcgaca tattccagca gccccaccag aaaccgcgct
     6901 cattgcgtga gtaccagtgg gtcagcaagc gggcacacgg tggccatccc cagccctgaa
     6961 aaaaaccgtt cagtgtccag accacaagca gaagcgtaag tgattcgcca aaggcaaaca
     7021 ccacgttcaa taacccggtg gcgaacaagc cgacgcccat aaaaccgcgt tgtccgtggc
     7081 tgtcatgcca cagtccggcg gtaaatttcg acaatccgta gctgagataa aacagcgaac
     7141 ccagcaggcc aatgtcgcct ttatccagtc cgaggtcggt ttgcagcgcc ggaaggacgt
     7201 aattcacgct ttttcgcgtc agataaaatg cggcgtagcc aataatcatg cacatcagca
     7261 atcgcggacg taatgtccgg tagcattgat taatttctct cgctgaacgc gcgtgcatgg
     7321 ttgtctccat gttgctgact atatgaaaat gaaagtgaaa ggggatgaga gaaaatccgg
     7381 gatacctaag actttttcct ggttatccgg cgatttgttg caaaaatgtg ggcaagttaa
     7441 caattacccg cgtgccgtgc tgtttttcga gcgttaagtc gccgcccagg gcgctgacgc
     7501 gctcacgcat cccctgaatg ccaaatccgg ccattttccc gctggcgatc cccacaccgt
     7561 tgtcgctgac ctccagatgc aatacctctc cctgctggcg caggataatc gtcacttcgc
     7621 tggcttcggc gtgtttacag acgttattga gcaactcctg caataaccga tacaaggtaa
     7681 agcgcaccgt ttcgttttct ggtgtggagt tcagttgata ggcaaactgg caatgaatgc
     7741 cgcgttcact aaaggcaaat tcattaatca gatgcagcaa cgcttcgcgg aatgtcagtt
     7801 catcaagcgc gggaggccgc aactggcgca gcaactggcg ggtggagaga tgaatgcggc
     7861 gggcgagttc attgatttgg ctggctgcgg attgaatttg cgccggatcg cgcgcccgct
     7921 tcaccagttg cgactggatc tgaatggcgg tgatgttctg accgatttcg tcgtgcagct
     7981 ctcgcgccag gtttttgcgc gtgtcctcct cggtgtggat cagcttttcc gccaatgccc
     8041 gccgcgctgc cagctcggcc tccagccgtt gtcggtaatg gtgcaggttt agcgccagat
     8101 gttgctggcg actgatggca atgcccaggc caattcccag caatgcctgg gtcgcgagga
     8161 aaatctccag ctccaccagg ttactaaagc ccacgccaat ctggcgggcg atggtgatca
     8221 tcatgctgcc caaaagcccg gagagtacgc caccttgcca gccaaatttc cacgccatca
     8281 ccacattggg tagaaaaact acgatcaaca gcaggcgttc aatttcaggt gacagcacca
     8341 tctgagtgcc gataccaatg ataaaaaaca ggctacacca gatgattaaa gaggtacgca
     8401 aggggggatt atgcgtgtca agccccagga gctggtagcg atgctgctgg cgcagaaact
     8461 caaagatgag atagacaaac ggcgtcagta atacgccgcc ggtaaatgag gtcaaccccg
     8521 gcagcattat tgggcctgac ataaaagggg agagcagggc ggtattcaac aatgaagctg
     8581 ccgtgacagt ggctaacagc aacgtcagac gctgccagta aagtggaaaa cggtgccaga
     8641 agttttgcgc aaggatggca ggaaacaggc taataaccgg cgttgccagc atgagataac
     8701 cgttcaatag ctgttcacta tggagccaga acatcatcag caaaggtggg agcaccagcg
     8761 caggccagta gcggcgagag agcaaaataa gaagggcgag gtaaaccccc tgcgggagaa
     8821 acaacaccgc ctgttgaccg ttattggtca gataaaagct gagcgtccac agcatcagcc
     8881 agccagttcc ccaggccagc acaataaaca gagaaatcac ccaatgacga aggttgcgcg
     8941 acattaatgc cccgccagca gatggtggtc gagcgcaaaa tgcaccagct caatggtact
     9001 atggcagttc agttttccca gcacattggc gcgatgcaca tgtaccgttt tatgactgag
     9061 ttcgagctta aaggcgatct ctttaacgct gtcacctttg accagtaaat caaacacttc
     9121 acgttctcgt ggggtgagaa tttccagcgc ctgggcgggt tgcccgccgc cgcgtaaagc
     9181 ccgaattgca tcggcacaga gataatgccc gcccagcccg accgagcgca ccgcctgtac
     9241 taactcttcc ggcccacagc gtttggtcaa atagccgctg gcaccagcat ccagcgcgct
     9301 ttgcacaaag gttggcgcgt cataaatgct aagaataatg gcgcgaaatt gcggtttttg
     9361 tgcacgcagg cgcttaagca aactgaggcc gttttcgtcc ggcatagcaa tatcaatgac
     9421 ggcaacattg acgtcatcac ggattaatgc cggccatgcc tgtgcggcac tactgtactg
     9481 accaatgacc tccagatcgt cttccagaga gagtaattgt gcgaagccag agcgcaccac
     9541 cacatggtca tccaccagca caacccgaat catacttttc ttctcttatt atctcattct
     9601 tctatcatgc atcacggcgc gtcgctggca atcaaccagc gacagatatg gaatggatgt
     9661 aacaaaaaat gtaatttgtt attcaatata attatgcttt atttcatgag tggctctata
     9721 tctggtgttc agattattgt ctgcaggggc agttcagaag atgtgagcaa ataaaaaaga
     9781 aaacataaat aaaaccatcc ggctttcggg gccagatggt tattcattat ttttatgtca
     9841 ctgattcatc aggtttgctc agaaagcgaa tcaatgtagt cagcatacca ctgcatcatt
     9901 tcacggcgac catcgatata cagggcatga ttataggtac ccctgatgga atttttatcc
     9961 acatgagcta actgcatttc aatccaggca gaattaaacc cctgttcgtg cagaatggta
    10021 ctcatggtgt gacgaaaacc atgaccggtt gc
//
LOCUS       DAZGHF010000157        10029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGHF010000157 DAZGHF010000000
VERSION     DAZGHF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.158"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            444..1421
                     /gene="glaH"
                     /locus_tag="ACHK3A_004264"
     CDS             444..1421
                     /gene="glaH"
                     /locus_tag="ACHK3A_004264"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO8742271.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATNHYQTHQ"
     gene            1441..2709
                     /gene="lhgO"
                     /locus_tag="ACHK3A_004265"
     CDS             1441..2709
                     /gene="lhgO"
                     /locus_tag="ACHK3A_004265"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO8742272.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALQEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2732..4180
                     /gene="gabD"
                     /locus_tag="ACHK3A_004266"
     CDS             2732..4180
                     /gene="gabD"
                     /locus_tag="ACHK3A_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO8742273.1"
                     /translation="MKPNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4194..5474
                     /gene="gabT"
                     /locus_tag="ACHK3A_004267"
     CDS             4194..5474
                     /gene="gabT"
                     /locus_tag="ACHK3A_004267"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO8742274.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYI
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGHKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5712..7112
                     /gene="gabP"
                     /locus_tag="ACHK3A_004268"
     CDS             5712..7112
                     /gene="gabP"
                     /locus_tag="ACHK3A_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO8742275.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7133..7795
                     /gene="csiR"
                     /locus_tag="ACHK3A_004269"
     CDS             7133..7795
                     /gene="csiR"
                     /locus_tag="ACHK3A_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO8742276.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKRDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7796..8245)
                     /gene="kbp"
                     /locus_tag="ACHK3A_004270"
     CDS             complement(7796..8245)
                     /gene="kbp"
                     /locus_tag="ACHK3A_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO8742277.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8329..8487)
                     /gene="yqaE"
                     /locus_tag="ACHK3A_004271"
     CDS             complement(8329..8487)
                     /gene="yqaE"
                     /locus_tag="ACHK3A_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO8742278.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8670..8969
                     /gene="ygaV"
                     /locus_tag="ACHK3A_004272"
     CDS             8670..8969
                     /gene="ygaV"
                     /locus_tag="ACHK3A_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO8742279.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8979..9503
                     /gene="ygaP"
                     /locus_tag="ACHK3A_004273"
     CDS             8979..9503
                     /gene="ygaP"
                     /locus_tag="ACHK3A_004273"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO8742280.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9550..9954)
                     /gene="stpA"
                     /locus_tag="ACHK3A_004274"
     CDS             complement(9550..9954)
                     /gene="stpA"
                     /locus_tag="ACHK3A_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO8742281.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2315 a         2568 c         2727 g         2419 t
ORIGIN      
        1 gtaaagcatt tatgcttaga ggcggaactc taaatatcaa tattcctggg cgtagcgctc
       61 ttcttttagg gaaaactgga gaaccgccga actatctcta tttgtaattt aatgttatat
      121 ctgccccgct aaaacggggc agataatatg tttagtttac taacggtcat tttgcagtga
      181 agccatttac cgttttttat cgaccagata atctgttctc tgatggtaat ccacccctaa
      241 cctgttgctt tagttattca tttcctgtct cactttgcct taataaccta cgtcaaatgt
      301 taccaatttg ttgtttttga tcgcaataag aaaacaatat gtaactttta tgcgcatttt
      361 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
      421 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
      481 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatct ccgcgtctgc
      541 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
      601 ccgtgcaggc gctggagtac aaatcttttc tgcgttttcg ggtaggcaaa attcttgacg
      661 atctgtgtgc gaaccagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
      721 gtgcgctgtt gatcaatgcg gtgggtgtcg atgatgtgaa gcaagcggat gagatggtga
      781 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
      841 agtattacgc gcgattcgtg gtgaaaaatg ttgataactc agacagctat ctgcgtcagc
      901 cgcaccgcgt aatggagctg cacaacgacg gcacctacgt cgaagagatc accgactatg
      961 tgctgatgat gaaaatcgac gagcagaaca tgcagggtgg aaattcgttg ctgctgcatc
     1021 tcgatgactg ggaacatctg gaccactatt tccgccaccc gctggcgcgt cgcccgatgc
     1081 gctttgccgc gcctccgagc aaaaacgtca gcaaagatgt tttccatcca gtgttcgacg
     1141 ttgatcaaca gggtcgcccg gtgatgcgtt atatcgacca gttcgtccag ccaaaagact
     1201 tcgaagaagg cgtgtggctg agtgagcttt ccgatgccat tgaaaccagc aaaggcattc
     1261 tttctgtgcc cgttcccgtt ggcaaattcc tgttgattaa caatctattc tggctgcacg
     1321 gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct
     1381 atttcgctta cgccactaac cactaccaga cgcatcagta agcgcaaagg aattgagcgg
     1441 atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg
     1501 attgatgttt atccggatgc ccgcattgcg ttgcaggaaa aagagtccgg cccggcctgt
     1561 caccagacgg gccacaacag tggcgtgatc catgccgggg tctattacac gcccggtagc
     1621 ctgaaggcac agttttgcct ggcgggaaac cgcgccacca aagccttttg cgatcaaaac
     1681 ggcattcgct atgacaactg cggcaagatg ctggtcgcca cctctgaact cgaaatggaa
     1741 cggatgcgcg cgttgtggga acgcacggca gcgaacggta tcgagcgcga gtggttaaac
     1801 gccgaggaac tgcgcgagcg cgaaccgaat atcaccgggc tcggcggcat ttttgtgccg
     1861 tccagcggca ttgtcagcta ccgcgaagtg acggcggcga tggcaaaaat cttccaggcc
     1921 agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc
     1981 gtggtgatac gtacccgtca gggcggtgaa tatgaagcat cgacgctgat tagctgttcc
     2041 gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt tattatctgc
     2101 ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt taaccacctg
     2161 atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac ccgcatgatc
     2221 gatggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgt
     2281 aagcgcgact tctcatttag cgacacgctg gagattttgg gctcgtcggg gattcgccgg
     2341 gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc
     2401 ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc
     2461 tggcccgctg gtgtgcgggc gcaggcggta tcgccggacg gcaagctgat tgacgatttt
     2521 ctgtttgtca ccaccccgcg cacgatccac acctgcaatg cgccctcccc ggcagcgaca
     2581 tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag
     2641 agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt
     2701 aatcaataac ctttgaaaac aggacgtaac aatgaaacct aacgacagta acttattccg
     2761 ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggcgagg tcatcgacgt
     2821 caccaatccg gcgaacggcg acaagctggg tagcgtaccc aaaatgggcg ctgatgaaac
     2881 ccgcgccgct atcgacgccg ccaaccgcgc tctgcccgcc tggcgtgcgc tcaccgccaa
     2941 agaacgcgcc accattctgc gcaactggtt caatttgatg atggagcatc aggacgattt
     3001 agcgcgcctg atgaccctag aacagggcaa accgctggcc gaagcgaaag gcgaaattag
     3061 ctacgccgcc tcctttattg agtggtttgc cgaagaaggc aaacgcattt atggcgacac
     3121 cattcctggt catcaggccg ataaacgcct gattgttatc aagcagccga ttggcgtcac
     3181 cgcggctatc acgccgtgga acttcccggc ggcgatgatt acccgcaaag ccggtccggc
     3241 gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct
     3301 ggcgctggcg gagctggcga ttcgcgcagg cgttccggct ggcgtattta acgtggtcac
     3361 cggttcggcg ggcgcggtgg gtaacgaact gaccagcaat ccgctggtgc gcaagctgtc
     3421 gtttaccggc tcgaccgaaa ttggccgcca attaatggaa cagtgcgcga aagacatcaa
     3481 gaaagtgtcg ctggaactgg gcggcaacgc gccgtttatc gtctttgacg atgccgacct
     3541 cgacaaagcc gtggaaggcg cactggcctc gaaattccgc aacgccgggc aaacctgcgt
     3601 ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg aaaaattaca
     3661 gcaggcggtg agcaaactgc acatcggcga cgggctggag aatggcgtca ccatcgggcc
     3721 gctgattgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa
     3781 aggtgcgcgt gtggtttgcg gcggtaaagc ggatgaacgc ggcggcaact tcttccagcc
     3841 gaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc
     3901 ccttgccccg ctattccgct ttaaagatga agccgatgtg attgcgcagg ccaatgacac
     3961 cgagtttggc ctggccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtggg
     4021 cgaggcgctg gagtacggca tcgtcggcat taatactggc attatttcca atgaagtggc
     4081 cccgttcggt ggcattaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga
     4141 agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaacg cgaatgagca
     4201 gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc
     4261 accccatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt
     4321 atattgattt cgcgggcggc attgcggtgc tcaataccgg gcacctgcat ccgaaagtgg
     4381 ttgcggcggt ggaagcgcag ttgaaaaagc tgtcgcacac ctgcttccag gtgctggctt
     4441 acgaaccgta tctggagctg tgcgagatta tgaatcagaa ggtgccggga gatttcgcca
     4501 aaaaaacgct gctggtaacg accggttctg aagcggtgga gaacgcggtg aaaatcgccc
     4561 gcgccgccac caaacgcagc gggaccatcg cctttagcgg cgcgtatcac gggcgcacgc
     4621 attacacgct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg ggactgatgc
     4681 ctgggcacgt ttatcgcgcg ctttatcctt gcccgctgca cggcatcagt gaagatgacg
     4741 ctatcgccag cattcaccga atttttaaaa atgacgctgc gccggaagat atcgccgcca
     4801 tcgtgattga gccggttcag ggcgaaggcg gtttctacgc cgcgacgcca gcctttatgc
     4861 agcgtttacg cgcgctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga
     4921 gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtt gcgccggatc
     4981 ttaccacctt tgcgaaatcg atcgcgggcg gcttcccgct ggcgggcgtc accgggcgcg
     5041 cggaagtgat ggatgccgtc gctccaggcg gtctgggcgg cacctatgcc ggtaacccga
     5101 ttgcctgcgt ggcagcgctg gaagtgttaa aggtattcga gcaggaaaat ctgctgcaaa
     5161 aagccaacga cctggggcat aagctgaaag acggattgct ggcgatagcc gaaaaacacc
     5221 cggagatcgg cgatgtacgc gggctgggag cgatgattgc catcgagctg tttgaagatg
     5281 gcgatcacag caaaccggac gccaaactca ccgccgagat cgtggcgcgt gcccgcgata
     5341 aaggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccgc
     5401 tcaccattga agacgctcag atccgtcagg gtctggaaat catcagccag tgttttgatg
     5461 aggcgaagca gtagatccgt acccatgccg gaggcgacgc tgcgcgtctt gtccggccta
     5521 cgcgaatcca ggtcggataa agcgtttacg ctgcatccga caatctgtac gtgaacagga
     5581 ataaatctat gtcggccgga taaggcggag ccgctctccg gtaaaaagaa ccaataacaa
     5641 ttacacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga
     5701 gaggattcag gatggggcaa tcatcgcaac cacatgagtt aggcggcggg ctgaagtcac
     5761 gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca
     5821 gcgtcgccat cgccgaagcg ggcccggcgg tattactggc ctatctgttc gccggattac
     5881 tggtggttat gattatgcgg atgttggcgg aaatggcggt tgccacgccc gataccggtt
     5941 cgttttccac ctatgccgac aaggcaattg gccgctgggc gggttatacc atcggctggc
     6001 tgtactggtg gttttgggta ctggttatcc cgctggaagc caacatcgcc gccatgatcc
     6061 tgcactcgtg ggttccaggc attcccatct ggttattttc cctggtcatt accctcgcct
     6121 tgactggcag taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc
     6181 tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt
     6241 tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc
     6301 ccaacggctt cggcgcagta ttaagcgcga tgttgatcac catgttctcg tttatgggcg
     6361 cagaaattgt caccattgcc gccgcggaat ctgacacgcc ggaaaaacat attgtccgcg
     6421 ccaccaactc ggttatctgg cgtatttcta tcttctattt atgctctatt tttgtcgtgg
     6481 tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat cgctcggttc
     6541 tggagttgct caatattccc catgcgaaat taatcatgga ctgcgtgata ttactttccg
     6601 taaccagttg cctgaactcg gcgctgtata ccgcttccag aatgctctac tccttaagcc
     6661 gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc ccgtatgtgg
     6721 cggtattact ctcgaccggt gcggcatttt taacggtggt ggtgaattat tacgcgcctg
     6781 cgaaagtgtt taaatttctg atcgatagct ccggcgctat cgccctgctg gtttatttag
     6841 tcattgccgt ttcacaattg cggatgcgca aaattctgcg agcagaagga agcgaaattc
     6901 gcctgcggat gtggctttat ccgtggctca cctggctggt catcggtttt ataacctttg
     6961 tgttggtagt gatgctattc cgcccggcgc aacagttaga agtgatttcc accggtttat
     7021 tagcgatagg gataatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt
     7081 ggcaaaaaac acccgttcat aatacgcgct aataatgatc aggagtcaca ccatgaccat
     7141 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggaa actttcaacc
     7201 ggatgaaaaa ttacggatga gtttgctgac atcgcgttat gcacttggcg ttgggccgtt
     7261 gcgggaagcg ctttcgcaac tggtagcaga acgactggtc acggtggtga atcaaaaagg
     7321 gtatcgggtg gcgtctatgt cagagcaaga gctgctcgat attttcgacg cccgcgccaa
     7381 tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga
     7441 cgttctcgca aaagcgcatc tgctcagtaa gctggaggcc tgtgacgcca gtgagaaaat
     7501 gcttgatgag tgggatctgc gtcatcaggc gtttcatact gcaattgtgg cgggctgtgg
     7561 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt
     7621 tatctggctg cggcgaacag tgctttcggt ggaaatgctg gaagataaac gcgatcagca
     7681 ccagaccctc actgcggcgg tactggcgcg agataccgcg cgcgccagtg agttaatgcg
     7741 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
     7801 cttcaggaat acgcaacact tgccccgggt aaattttatc cgggcttttg agcatcggtt
     7861 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
     7921 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagcggct ggtgtcgccg
     7981 ttttcacctg atcatcgaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
     8041 tctccttcgc ctcctgactt aagccgtcac ccgtgaccgt cgctttgccg tcggcgattt
     8101 gaatgttcac tttatcggca tcaggtattc cggttttgtt cagatgctcc tgcaccttct
     8161 tcgcctgatc gtctttatcg tgctgacctg taaccgcgtc ccagagtttt tctccggcat
     8221 ctttcacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
     8281 tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga acacaggttt aatcgcgggt
     8341 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
     8401 aataatgaac gcccaaccga accctttacc gagcagcacg ccgagcggcg gcagaatgat
     8461 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
     8521 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag tttcagctta
     8581 cgcttttact ctcatttacg ctgctgaggc tggtatatgt tgcattttta gtttagagtt
     8641 tacttaattt agaaaatact taaatatcta tgactgaact cgcgcaatta caggctagtg
     8701 ccgaacaggc agcggcctta ttgaaagcaa tgagccaccc taaacggttg ctgattctgt
     8761 gcatgcttag cggttccccc ggcaccagtg cgggagagct gacgcgcatt accggactga
     8821 gtgcctctgc aacatcacag catctcgctc gtatgcggga cgaagggctt atcgacagcc
     8881 aacgggatgc ccaacgcatt ctatattcca ttaaaaatga ggcggtaaat gccattatcg
     8941 ccaccctgaa aaatgtctat tgtccgtaag gagtcactat ggctttgaca accatttcgc
     9001 cgcatgatgc acaagaatta atcgcacgcg gcgcaaagtt aatcgatatt cgtgacgctg
     9061 atgaatatct tcgtgaacat attcctgaag cagatctggc tccattatcc gtgctggaac
     9121 agtcaggtct tccggctaaa ttacgtcgcg agcaaataat attccactgc caggctggta
     9181 aacgcaccag taataacgcc gataaattag cagcgattgc cgcccccgca gaaatctttt
     9241 tactcgaaga tgggattgat ggctggaaaa aagcaggatt accagtagcg gtaaataaat
     9301 ctcaaccctt gccgttaatg cgccaggtgc agatcgctgc gggtggttta atattaatcg
     9361 gcgttatact aggttatacc gtaaatagcg gtttcttctt attaagcggc tttgttggtg
     9421 ccgggttact gtttgcagga attagcggtt tttgtgggat ggcaaggttg ttagataaga
     9481 tgccgtggaa ccaacgagct tgagaagcga cgccggacgc gccatagcag caacatccgg
     9541 cctcagtaat tagatcagaa aatcgtcgag agatttacct tctgccagcg cctgagcgat
     9601 tggcttcggt gtacggccct gaccggtcca ggttttagtt tcaccgttaa catcgatgaa
     9661 tttatatttc gccggacgcg gctggcgttt tttaccagcg cgtggtgccg cagcagagct
     9721 attacccaat aactcttccg ggttaattcc gtcagctttc atcagctcca gccaggtgct
     9781 aattttttcc tggcgctctg ccagttcacg ctgctgttgt tcttcttctt cacgtctttc
     9841 tttagtgaca accctgaatt tttcgagcat ttcttcaaga acgtcaatgg agaattcgcg
     9901 agccatcgca cggagggtgc gaatgttatt taaactttgt aacattacgg acataataaa
     9961 gaaaaccttt taacgccaaa acaaaaaagt atttcgtcgc gtagtgtaag cgatcactta
    10021 ttatatttc
//
LOCUS       DAZGHF010000158         9935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGHF010000158 DAZGHF010000000
VERSION     DAZGHF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.159"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(54..779)
                     /gene="phoU"
                     /locus_tag="ACHK3A_004275"
     CDS             complement(54..779)
                     /gene="phoU"
                     /locus_tag="ACHK3A_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO8742282.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(794..1567)
                     /gene="pstB"
                     /locus_tag="ACHK3A_004276"
     CDS             complement(794..1567)
                     /gene="pstB"
                     /locus_tag="ACHK3A_004276"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO8742283.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1658..2548)
                     /gene="pstA"
                     /locus_tag="ACHK3A_004277"
     CDS             complement(1658..2548)
                     /gene="pstA"
                     /locus_tag="ACHK3A_004277"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO8742284.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIAAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2548..3507)
                     /gene="pstC"
                     /locus_tag="ACHK3A_004278"
     CDS             complement(2548..3507)
                     /gene="pstC"
                     /locus_tag="ACHK3A_004278"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO8742285.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALTVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3594..4634)
                     /gene="pstS"
                     /locus_tag="ACHK3A_004279"
     CDS             complement(3594..4634)
                     /gene="pstS"
                     /locus_tag="ACHK3A_004279"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO8742286.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4882..5952)
                     /locus_tag="ACHK3A_004280"
     CDS             complement(4882..5952)
                     /locus_tag="ACHK3A_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8742287.1"
                     /translation="MNKYIIPWCFIVFIFSFSGATFAAPKGICTSDNGSFHSTLDFSG
                     YLITADENRVGKTFNKTVTNGDSYPAHCHCDTGNVGEFPYIYYTARINESLGYAGVRS
                     NVNYFNLNPNLDVGISIDLLGVGYINAPFEYHANRPTGGSYKCSRTDPLSISSGAKAI
                     IYFYIKKTFAGKLIIPETLVAKLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINS
                     LRPINFDFKEIPAADFSSVVGSTVTTHKITKTVTIECENLGILNTDDISTSFYATEPS
                     TDNSMVVTSNPNVGIKIYDKNNKEINVNGGELPTDMGKSTVFGEKAGSVTFSAAPASL
                     TGARPAPGTFTATATITIEIVR"
     gene            complement(5963..8485)
                     /gene="lpfC-O113"
                     /locus_tag="ACHK3A_004281"
     CDS             complement(5963..8485)
                     /gene="lpfC-O113"
                     /locus_tag="ACHK3A_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO8742288.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQSLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFVAIKKTLTTFGVK
                     VDALKSLNDVDETVCIDPGPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPSRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTTDGS
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQVQILHGFPYGFTLYGGMQA
                     AEKYGSAALGVGKELGALGAISFDVTHARAKFSHADTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNNPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGTYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTRVGYRVLFRVAGTKGKPAPFGAIATVQNTSSADSGIVGDQGELYLSG
                     LPEKGQVMLSWGENAATTCTFDYSLSIPESESGLIEQGVTCH"
     gene            complement(8510..9241)
                     /gene="lpfB-O113"
                     /locus_tag="ACHK3A_004282"
     CDS             complement(8510..9241)
                     /gene="lpfB-O113"
                     /locus_tag="ACHK3A_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO8742289.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGT
                     RVVYLSNNPDKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPAQEK
                     VLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRL
                     IPEDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(9289..9861)
                     /gene="lpfA-O113"
                     /locus_tag="ACHK3A_004283"
     CDS             complement(9289..9861)
                     /gene="lpfA-O113"
                     /locus_tag="ACHK3A_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO8742290.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQTVPLGKVSKNAFSGVGSLASPQQFSIKLEKCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         2556 a         2520 c         2320 g         2539 t
ORIGIN      
        1 aagttatata taacaaatcc caataattaa attattggga tttacttggt gaattattta
       61 tcgctatctt tttctgccag cagtttatcg agctcgtcac caccgacgtg acggaaatcc
      121 tgccccttca cgtagtagaa gataaactca caaatattct ggcagcggtc gccgatacgt
      181 tcgattgaac gcgcacaaaa cagcgcggta agtacgcttg gaatggtgcg cgaatcttcc
      241 atcatgtagg tcatcagctg acgaacaata ccttcgtact cctgatcgac ttttttatct
      301 tcacgataaa tacgtaccgc ttcatcaatg tccatccgcg cgaacgcgtc cagcacgtcg
      361 tgcagcatct ggatggtatg acggcccagc gactccagac ttaccagcaa tggctgatgc
      421 tgctgggaga atttctccag tgcagtacgg cagattttgt ccgccacgtc gccaatacgc
      481 tccagctcgg caatggtttt actgatcacc ataaccagac gcaggtcgct cgccgtcggc
      541 tggcgtttgg caataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg
      601 ttcttgtcgc cttcgatgac gcgcttcgcc agatcgctgt cctggttgtg cattgcagtg
      661 atcgcatcag aaagctgctg ctccaccatg ccgcccatgg tcatcacctg cgtgcggatg
      721 ctttccagtt cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata
      781 acgcactcct gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg
      841 gcttggtgaa cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg
      901 ccgtgtggtc ggaacaacgc gcagcctgct gcatgttgtg ggtgacgatc accacggtgt
      961 aatcctgctt cagttcggta atcagctctt caatacgccc ggtggagatg gggtcgagcg
     1021 ccgaacatgg ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac
     1081 acaaacgctg ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg
     1141 tttcgttcca caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcagcac
     1201 gggagagctt ctcaaacaga cgcacgccaa aagcgatgtt gtcgtagatg gacatcggga
     1261 acggcgtcgg tttctggaac accatgccca ctttcgcacg cagcagggcg atatcctgag
     1321 agttggtcag gatgttgtcg ccatcgagca gaatttcacc ttccgcacgc tgttccgggt
     1381 acagttcaaa cattttgttg aaggtacgca gcagcgttga tttaccgcag ccggacggcc
     1441 cgataaacgc cgtaacctgg tttttggcga tatccaggtt gatgtttttc agggcatgga
     1501 atttgccgta gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca
     1561 tactcattgc aatcttttcc tcatcgtgcc tgatgcactt cgcgaatcag gcttaccgta
     1621 ggccggataa ggcgtttacg ccgcatccag caaagtgtta accgtgttta ttcttcgcaa
     1681 aaacaacgcg cgccagaatg ttcagcagca gtacgcacag ggtaatgatc aacaccccgg
     1741 cccaggccag ttgctgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg
     1801 gcaggttggc gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcagtaa
     1861 acagcagcgg cgcggtttca ccagcaatac gggcaatcgc cagcaggata ccggtcatga
     1921 tcccggacac cgacgctttc agcgtgatcg cggcaatcat cttccacttc ggcgtaccca
     1981 gcgcataagc cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc
     2041 ggataacaat cggcacctgc aacagcgcca gcgcaatcac gcccgcccag ccggagaagt
     2101 gctccatctg cgccaccacg atggtgtaaa caaaaagacc aaccacaatc gacggtgcgg
     2161 agagcaaaat gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt
     2221 cagccagata aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa
     2281 ttaacaggcc gctacccgcc agggcgttcg ccagaccacc gccttccgta tttggcggcg
     2341 gcgtcatttc ggtgaacagc gccagcgaca taccatcgat accgcgagtg atggtggaca
     2401 ttaaaatcca gatcagccag aacagaccga aggccatcgt cgccatcgag agcgtcagcg
     2461 caatacggtt cttaaggcgg cgacgcgcct gcattttacg gcgggattca gccagcgccg
     2521 cagtggtttg catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat
     2581 aatcataaac ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag
     2641 ttccatcagt gcagcaacat gcagacccga ctctgcttcc gcaaattcgt tggccagcgc
     2701 agaggtgata ctgttgcccg gcatatacag cgaggcgctg tcgagttggt aggtgttacc
     2761 gatgataaag gtcaccgcca tggtttcacc gagcgcgcgc cccagcccca acatgatgcc
     2821 gccgataaca ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt
     2881 gcagccaata ccgtaggccg actctttcat catcaccggg gtttgttcga atacatcgcg
     2941 cattaccgcc gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat
     3001 accgatacca aaggccgggc cggagaacag cgcgccgaca atcgggatat tcgacatgat
     3061 attgccgacc ggctcctgga agtaaacggc gaacagcggg gcgaagataa acaagcccca
     3121 catgccgtaa acgatacttg gaatggcagc cagcagctca atggcgatac ccagcgggcg
     3181 tttcagccag ccaggcgcaa gctcggtcag gaacagggcg ataccaaaac tcaccgggac
     3241 ggcgatcagc agcgcgataa acgaggtcac cagcgtaccg tagatcggca ccagcgcccc
     3301 gtagatatcg ttcggcgcat cccactcttt ggtccacagg aaagccagac caaatttctg
     3361 aatgctcggc caggatgaga tgatcagaga gacaataatg ccacccaaca tcaatagcac
     3421 agtcagcgcc gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg
     3481 gttaaaagca ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt
     3541 cattttgtaa tgccggatgc ggcgtaaaac accgtacccg gcctggagtt ttattagtac
     3601 agcggcttgc cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact
     3661 acgctatccg gcaggctggc ataatccagg tcgttcgcct gtttagcccc ggttttgtat
     3721 gcccagtcga agaacttcag cacttcggtg ccttgttctg gtttcttctg atctttatgg
     3781 atcagaatga acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga
     3841 tcctgagcga atgttttgct ccagtctgcg ccttttgctg cattagcgaa gttttcttcg
     3901 gtcggactca ccggtttgcc atcagcggag atcagtttgg tgtacgccag gttgttctgc
     3961 ttcgcgtaag catattcaac gtaaccaatt gcacccggca gacgctgaac gaacgcggcg
     4021 ataccgtcgt tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca
     4081 acgttgtttt tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggaagtc
     4141 ccggagccat ctgcgcggcg tactacagca atgttctgtg aaggcagttt cagacccgga
     4201 ttcagtttgg cgatggcttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg
     4261 agggttttac catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc
     4321 accacgccgc caatcacggt cgggaactgg aacaggcctt cctgagccag tttttcgtca
     4381 gacagcggcg cgtcagaggc accgaaatca acggtattag cgataatctg ttttacgcca
     4441 ccggaagaac cgataccctg gtagttaact ttattaccgg tttctttctg gtaagtgtca
     4501 gcccatttgg catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct
     4561 gcaaacacag agaaagcact catcgataag gtcgcggcga caacagttgc gacggtggta
     4621 cgcataactt tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag
     4681 aagcaaaata ggacaaacag gtgacagtta tatgtaaggt ttatgacagt tttatgacaa
     4741 atcagaatta ttaattaact ttttgatttt attgactaag aaaaagaaaa gtaatgtcat
     4801 ctttttgtca tgcagagtga aaactcgcgg acaaaaatgc gtaagatgac aacggtgtga
     4861 ggaacaccgc tgtcagttcg tttagcgtac aatctcaatt gttatcgttg cagtcgcggt
     4921 aaatgttcct ggcgcggggc gagcccccgt aaggcttgca ggagcagcag aaaaagtgac
     4981 gctaccggct ttttcaccaa agacggttga tttacccata tctgtcggca gttcaccacc
     5041 gttaacattg atttccttat tatttttatc ataaatttta atcccaacgt tcgggtttga
     5101 tgtcacgacc attgagttgt cagtactggg ttcggtagca taaaaagagg tactgatatc
     5161 atcagtattt agtattccta aattttcaca ctcaatggtg acggttttgg taattttatg
     5221 cgttgtcacg gtacttccaa ctaccgaaga aaaatctgcc gcaggaattt ctttaaaatc
     5281 aaaattaatg ggtcttaaac tgttaatttc acagctttgc ggtgcagtga tatcgccacg
     5341 aatataaaca tccgccatag gttgtgagta atcaaccgga gtgtcacggc ttatggttcc
     5401 gtacaatttc gccacgagag tttcaggaat aattaacttt ccagcaaaag tcttcttaat
     5461 ataaaaataa attattgctt ttgcaccact ggaaatactt aatgggtccg tgcgactaca
     5521 tttatatgaa cctccagtag gcctgttagc atggtattca aaaggtgcat taatgtatcc
     5581 tacaccgaga aggtctatcg atattccaac atccagatta ggattcaaat taaagtagtt
     5641 tacattagaa cgaacacctg cataacctaa ggactcgttt attctcgctg tataataaat
     5701 ataaggaaac tctcccacgt tccctgtatc acaatggcag tgagcaggat aagagtcccc
     5761 atttgtcacg gttttattaa aggtctttcc cactctgttc tcgtctgccg taatcagata
     5821 gccggaaaaa tcaagtgtgc tatgaaagga cccattatct gaagtgcaga taccttttgg
     5881 ggccgcaaat gttgcgccac taaaggagaa gataaaaaca ataaagcacc atggtataat
     5941 atacttgttc ataaaaatcc ttttaatgac atgtcacacc ttgttcaatt aagccgcttt
     6001 cactttctgg tattgaaagt gaataatcga aggtgcatgt agtggcggca ttttctcccc
     6061 aggagagcat cacctgccct ttttcaggaa ggcccgagag ataaagctcc ccctggtcac
     6121 cgacaattcc tgaatcagcg gagcttgtat tttgtactgt agcaatagcg ccaaaaggtg
     6181 ctggtttacc ttttgtaccc gcaaccctga aaagcacacg ataaccaaca cgagtaacaa
     6241 attcggcctt aaccactgca ccacgggtgg gaaccatcgt aacgacggta ttatcaagtt
     6301 ctatattcga cttcgagaaa tagttatcgc tgattgctac ccggttttca tcataagggt
     6361 ttagctgtgt cttgaccgta tagccgcgcc agtccgtcgc agcaccgcgc atattgtcta
     6421 tttctaaccc ggcagcccct ggtgctttaa caagagctat ggtttcaccc aactcctggc
     6481 ttaacgttac gccttcgctg tgcgccagaa ttccccctct gataccataa ttcagcctct
     6541 gagaattttg gctgtagtta tagctgccgt tcaccgtgcc ataagtgcca tcataggtca
     6601 gggacatatt accgccaacc tcatcatcat ggctgttata ggactgcatt aaattccagg
     6661 aaaggttatt tctttccagc gccgtaccgc tgagtccaag ttgatgtgag ctaccggaat
     6721 tatcgttttg tgaaacagaa taggtggcgt aactatttcc ccaccagccc gataaaggaa
     6781 cagataaaga gagcatgtag atctgctcac tggtgttatc actggcatgg ttgttccccg
     6841 tcccctgccg ggcagtattg gaataactcc aggagacgtt ccacgagaga cgcttccagc
     6901 tactgctgta gccaaattgc attaatcgtt caacatcatc ggtatgccag tactgctggc
     6961 gacttaatgt cagatataag ttgccatagt ctctgcccag tgattgcgtt aacgttcctt
     7021 ccacacggct gcggcggtta ccggtttccc agaaatcttc aggattatta cgccgcgacg
     7081 cccattcatt gagagtatag taaccttcgg tggagtaacg atagccaacc aggcgcaagc
     7141 tggtatccgt atcgtcaaag cgtttcgaat acagaaagcg ataagactga ccggtttccg
     7201 tatcagcatg gctaaatttc gcacgggcat gtgtcacatc gaaagaaatt gcacccagtg
     7261 cgccaagctc tttaccgaca cccaaagcgg cagaaccata cttttcagca gcctgcatac
     7321 ctccatacaa ggtgaaaccg tagggaaaac catgaaggat ctgcacctga agaacgtcag
     7381 gggtaaagcc atcttcatcg cgtaattctc ccacactgac atcaacatca gtttgacctt
     7441 cacgtttgag gatggctaac gacgcataag gttgagtaaa tcgttgttcg ctaccgtctt
     7501 cttctttgat ggttacgtcc agatcaccac ctgtactggc ggtgttgagg tcggtaattt
     7561 caaaagcacc ttgtggaacg gctgactgat aaatcacgta gccattctgg cgaataatga
     7621 cagtggcgtt agactttgca atcccacgca ctacgggggc aaatccgttt tggctggcag
     7681 gcaacatatc gttatcggta tacaagcgtg cgccacgaat ttgcgtactg tcgaagatat
     7741 cgctcgccgt ccacgtatca cctatcataa tctggcttct taacgcagct atatcacgtt
     7801 gtatccaggt gcttatagag ttgtattctg cactgccatc ggtcgtattt aaggtgctgt
     7861 aattgcgtaa tcgccacggc ccaatattca gtccattgcg cagattgaga taactggtat
     7921 ctgtctcttg tgaaccataa tccgaacgta gagtatgtga accagaaaaa tcgtagttga
     7981 tggtgagcgc gttaatcccc tcatcccaac gcgaggggct gatgtaatca taagccatcg
     8041 catccatata gatttgggga atggagatat tcagttgcag tttatcgctg tcgaactgcc
     8101 atgatgagcc ttcaatgcgt ggcccaggat caatacagac tgtttcatct acatcattga
     8161 gcgatttcag cgcatcaact tttacgccaa aggtggttag cgttttttta atagcgacaa
     8221 agcaggcgtg taaatcagca tcgggattat cctgaacaaa ggtcacatcc tgacgttcaa
     8281 acattttacc gttgataaaa atatcaacgc tataaacacc agggggcatc aggtttgccc
     8341 gattaaactg gcgaatatcg actccggatt gatggtcgat ttccagaagc gccggattga
     8401 atacactgac ctcggccatc aggctttgcg agaaaatcag acacgtccct gccgtgaggc
     8461 caaccactat tctggtcgtc atcataatac gcctatcctt atcccttgct tatagcgtca
     8521 tttttatcgg gtcactgact gcgccgtaat cattaataaa tcgccaggtc atttgacctc
     8581 ccgcagaatt agcaggtaaa ggtaacgtga gcgtttcaaa aggacggatc atgttgaggg
     8641 gcacatcatg accatcaacg atgacatcca tcacactgat atgaatcggg ttgggattct
     8701 ctgcaatcag gtttcgccct tcccggtgcc acttcacctt cggtgcagca tcttcgggaa
     8761 tcaggcgaat gcttgccggt cgccagaaca gcttgatacg cgttttaact gcgatttcca
     8821 gtgagttcgc cgctttgttg ctcgcagagg gcggaatatt tttaatattc agccagaaga
     8881 ctgactcgcg atcatcaggc aatgacacac ctttggtatg aataatgcgt aatacttttt
     8941 cttgcgcggg ttctaatcgt gataccggag gaataacggt aaacggtgct ttgcttttat
     9001 cctctttatt aaacggatct acccatgcct ggatgagata aggaattttt tcttccttgc
     9061 taaatacaga aatagattta tctgggttat tacttaagta aactacccgt gtaccaccaa
     9121 tgacaacgcc tgcataggtt aatgctggtt ggactaaaaa aacactgagt ataatcgcgg
     9181 taaataactt acatgaaacg gttgttttaa agagcctgat taaacaactt tttctggaca
     9241 taacgactcc ctggccaaag ccaactgaat aaaaaggccc ttgataaatt atttcttata
     9301 ttcgacagta aattgtgaat cggcgttcgc cgtacccgcc gttacggttg cattggttgc
     9361 cacatagcgt gcaataaact tcatttcagc cttgccttca gcgtcgatag cggtataggc
     9421 agaatcgtta taaagtttaa cctgcgagtt atctgactgg ttaaaaatac gaataccgac
     9481 acccgttgcc gcaatcgctt gtcctgtacc ggtaaaatcg ccagggttgc ccgctgtaag
     9541 cggcgtaccc actttcaggt cgccgtcgcc gccaggcgct tctgtaccat caaaacgaac
     9601 ggctgcctga gtgtaggttg ccgggcactt ttcaagttta atactgaact gctgtggtga
     9661 cgccagacta cctacacctg aaaatgcatt tttactgact ttacccagtg ggacggtttg
     9721 atcggctgta tcggaggtaa cttcacaagt cgtgtcgaca atttcaccga cgaagtgaac
     9781 aacaccatct tctgccagtg catgtccggc cagcattaaa gatgccagag tgaatgcacc
     9841 gcctataata ttacgcttca taaaaactcc attgatataa tatatccttt accatgaatc
     9901 atcacttata ttaataagtg tatttttgat ccaaa
//
LOCUS       DAZGHF010000159         9821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGHF010000159 DAZGHF010000000
VERSION     DAZGHF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.160"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(59..1219)
                     /locus_tag="ACHK3A_004284"
     CDS             complement(59..1219)
                     /locus_tag="ACHK3A_004284"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO8742291.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(1225..1896)
                     /locus_tag="ACHK3A_004285"
     CDS             complement(1225..1896)
                     /locus_tag="ACHK3A_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO8742292.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCIAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(2044..3525)
                     /gene="tolC"
                     /locus_tag="ACHK3A_004286"
     CDS             complement(2044..3525)
                     /gene="tolC"
                     /locus_tag="ACHK3A_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO8742293.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            3730..4359
                     /gene="nudF"
                     /locus_tag="ACHK3A_004287"
     CDS             3730..4359
                     /gene="nudF"
                     /locus_tag="ACHK3A_004287"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO8742294.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            4360..4782
                     /locus_tag="ACHK3A_004288"
     CDS             4360..4782
                     /locus_tag="ACHK3A_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO8742295.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            4807..5634
                     /gene="cpdA"
                     /locus_tag="ACHK3A_004289"
     CDS             4807..5634
                     /gene="cpdA"
                     /locus_tag="ACHK3A_004289"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO8742296.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            5634..6215
                     /gene="yqiA"
                     /locus_tag="ACHK3A_004290"
     CDS             5634..6215
                     /gene="yqiA"
                     /locus_tag="ACHK3A_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO8742297.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            6244..8136
                     /gene="parE"
                     /locus_tag="ACHK3A_004291"
     CDS             6244..8136
                     /gene="parE"
                     /locus_tag="ACHK3A_004291"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO8742298.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFTLWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(8181..9050)
                     /locus_tag="ACHK3A_004292"
     CDS             complement(8181..9050)
                     /locus_tag="ACHK3A_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HFO8742299.1"
                     /translation="MNNKFKIILWMMLSALPALSYAQVKNIVTGEYSAVSKIITEKET
                     LYPSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISML
                     YELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRK
                     KEDTLPPFEKGKLKRSWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQAN
                     IHAMTKMFSKDKWSSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLA
                     TLFPDRNKLCPIK"
     gene            complement(9064..>9821)
                     /locus_tag="ACHK3A_004293"
     CDS             complement(9064..>9821)
                     /locus_tag="ACHK3A_004293"
                     /inference="COORDINATES: protein motif:HMM:NF017942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO8742300.1"
                     /translation="FILKGNIMNSYLKISLAGITMLSCLSPTLVCAEQPHGFIDYRHE
                     YLDDTRTHADRVEFGTFFSNGIGLMGELRYNTDEGDKDKWDPSQFGNNGTGLSVVYRF
                     KPLDDKKFWLEPMFWLDTTQYWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKG
                     YGNDDRNNRRYDVWIDYRPQKTNFQYQLNLVYYNNGYLTWNDGHKNYTASFKVGYKMG
                     SWIPYMSIADYKGVDKTSSNRQIRWRWGLTYTF"
BASE COUNT         2350 a         2383 c         2556 g         2532 t
ORIGIN      
        1 attatgaatg gtggattata agagattgcc ccgcttatcg gggcaatatt aagctgtatt
       61 actcaacaaa aatatgcgga taaaaccgag acatatcctg ggtgatcaat gcacggtctt
      121 cacgaatgcc aattccggca ggttgatcgt tcaccagcca gctaccaatc agcatatagc
      181 tgtcgccgaa tttcggtaac gggtggaatt gctgaacaat catcccttct tcgccatacg
      241 gaccttccgc tgcttcaatg gttttgccgt tctcaatgat cgacacgttt gcgccttcac
      301 gggagaagat cggtttaacc acatattttt ccatttgcgg atgatcatct tccgcaaaat
      361 aagcaggcag caggttcggg tgattcggga acatctccca cagtagcggc agaagcgctt
      421 tgttggagat aatgctcttc cacgccggtt ccagccagcg tacgcctgca tcctccagct
      481 tggtggagaa catctcgcgc agcataaatt cccacggata cagcttgaac aggttggaaa
      541 ttacctgatc ctgtaaatcc gtgaactgac ctttttcgcc taaaccgata tcgtcgatgt
      601 agaggaattc ggtagcgatt tcagcttccg tcgcgcagtc ctgcaaatac tgaatggttc
      661 cgcgatcttc caccgtgtcg cgacagcagg tgagatgcag caactggaag ccatactgtt
      721 cacgcaactc gacaaagcga tcgattagct tttcttgcag actgttaaac tggtcgctgc
      781 cctccggcaa gttaccggcg ttaagctgat cttccagcca gatccactga aagaacgccg
      841 cctcgtatag tgacgttggt gtatcggcgt tattttccag cagtttaggt tcgccagtgc
      901 catcccacgc cagatcaaga cgcgaataaa gcgatggctg gtgagtcagc catgactgac
      961 gcacaaaact ccaggtgtgt tttggaatgc ggaatttggt catcagctca tcgctggcga
     1021 tcactttttc caccactttc aggcacatct ggtgcagttc ggcggtgact tcttccagct
     1081 tttcaacctg ggcgagggtc aacttgtagt aagcatcttc acaccagtac ggctcgccgt
     1141 acatggtgtg aaaattgaaa ccgtattcgt gggctttctc acgccagtcc gggcgctcgg
     1201 taatactgac tctttccatc ggtatcagcc acccattgaa cgagaagagg tgccggttgc
     1261 gctacgctgc atagtgcttt gtttggcaac agattcacca aaaccgccac gggtaacggt
     1321 agtggtggtc gccggttttg gtgccatggc tgtcttcggt acggtcatgg tgcggcctgg
     1381 ctgggctgcg ccatagtttt tacccgtcgc gtcggtatat ttaccgtaag ccgggctggc
     1441 tgggtttttc gaggagaaca gcggctgctg tgcaaatccc gcaccgccgc ccatcagacg
     1501 acccatcatg taaccggcca tcagcggcat ccagaaactc ccgctggatt gctgggcctg
     1561 cgcctggttt tccggtgcca tgccagcctg ggctggtgcc tgctggcact gaccttcgcc
     1621 aaattcagca atacagtctt cacgagtggc gtatttcggc gcagtacgtt cggcttcttt
     1681 cagcgcattg ttgtacgcgg tggtacattc ggcgcttttg cctgggtttg cagctgaaca
     1741 gtcgtcagca ttttgataga gagacactgt ttcatcactc ttttcacagc cagccagcat
     1801 aaaaacagtg gcaaccgcga gagcgactgg tgtcagatgg cgtgcgctcc agtttttgcg
     1861 gaacgatgcg tggcgtatgg attttgtccg tttcattttt gtcttccggg accagtggta
     1921 aatacccatc agaatagagg atggctggtc gaaattgaag cgagaagggg gaagaatgcg
     1981 gcagataacc cgtatcttta cgttgcctta cgttcagacg gggccgaagc cccgtcgtcg
     2041 tcatcagtta cggaaagggt tatgaccgtt actggtggta gtgcgtgcgg atgtttgctg
     2101 aacgacgggt gccgggctat caggcgcata accatcagca atagcattct gttccggcgt
     2161 ttgcggggca acgttttccg gattagtgga aaccggtttg ctcagcgcat tgttcagtgc
     2221 cagaagatcc tgctcgttca acgtacccag agctgattta atattcaact ggttaatcag
     2281 gtagttataa cgcgcattcg ccagctcttg cttggcgttg tacagcgtgg tggtcgcatc
     2341 caacacatca acaatggtac gcgtaccgac cgagtaaccc gcttccatcg cgtctaatga
     2401 gctttgagcg gaaactacgg cttgtttgta ggcgttaatg ctactgatag aagcattaat
     2461 gttgttgaag gaggaacgta cggtttgcac gacgctacga tgcgcgcttt ccagttgctc
     2521 gctggcacca acaaagttgt actgggcctg tttcacctgc gagttaacca ttccgccctg
     2581 ataaatcggc agcgagaagc tcaggccaac tttgttctgg cccatattgc tgtcgtcata
     2641 ctgggtacca gcggcaccac gggttttcga accgctataa gaggtgtcag aaatcccggt
     2701 agaagccgtt aaatccagag tcggtaagtg accatcctgc gcctggcgaa tttgctcgcg
     2761 cgccaggtcc tggctcaagc gcgcctgtaa cagcgacagg ttgcgttttt cggcttcttt
     2821 cagcagcgtg ttaaccggct gtggtttgtc ggttttaaag ttttcgacat tcagcgccgc
     2881 cagttctgga tagtagttac cggtgatctg gcgcagctgc tctaccgcgt tatcaaggtt
     2941 attacgtgcg gtcacttcgt tcgccagcac ggtatcgtac tgtgcgcggg cgttctgcac
     3001 gtcagtgatc gctaccaggc ccacgttaaa acgttgggtg gtttgatcta attgacggta
     3061 gatcgcttct ttttgcgcct gtgtatagga aagaacgtca atagcattca acacgttgaa
     3121 ataagcggtc gcggtgttga ggatcaaggt ttgttgatcg gtctgatacg tgacgtcctg
     3181 aatcccagct gctttttcct gcagcgttaa cgcacgccat ttcgacatat caaaaatgga
     3241 ttgagttaac tgcagggacg cactggtcgc gttagagttg atgccgttcg cgtcgcggta
     3301 gccgttgcta taggtgtaat ctgcacctaa acctagctgt ggcagtaatg gactgcgcgc
     3361 ttcattaatt ttttcaaagg cagcatcacg atcggcggca gacttacgca attccgggtt
     3421 actaaggcgt gcttgctgat aaacttgcat caggttctcg gcctggctca acgaactgaa
     3481 accagaaagg ctcaggccga taagaatggg gagcaatttc ttcatttgca ttccttgtgg
     3541 tgaagcagta tttagcgcga tcaaactgta aaattattgc ggattctagc agaagccgct
     3601 accgcaaaag gttggcgtta cgtgccatag ggcgttaatg tgcctcaatt taacacatga
     3661 ctgctcaaac ggcagtcaaa cgtcgctgaa attcacattt aattcactat tagtgccagg
     3721 acattaacaa tgcttaagcc agacaacctg cccgttacat ttggcaaaaa cgatgtagaa
     3781 attattgcac gagaaacact ttatcgcggc tttttttcat tagatcttta tagatttcgt
     3841 catcgtctat tcaacgggca aatgagtcat gaggtacggc gggaaatttt tgagcgcggt
     3901 cacgccgcag tcttgctacc ctttgaccca gtgcgtgatg aagttgtgct gattgagcag
     3961 attcgaattg ccgcgtacga cactagcgaa accccctggc tactggagat ggttgccggg
     4021 atgattgaag agggtgaaag tgtggaagat gttgcccgtc gcgaagcgat tgaagaggcg
     4081 ggactgatag tcaaacggac caaaccggtg ttaagtttcc tggcaagccc ggggggcacc
     4141 agtgagcgtt cgtcaattat ggtgggcgaa gtggacgcca cgaccgcaag cggtattcac
     4201 ggtctggctg atgaaaacga agatattcgc gttcatgtgg taagccggga acaggcatac
     4261 cagtgggtag aagaggggaa aatcgacaac gcagcgtcgg tcatcgcttt gcaatggctg
     4321 cagctgcatc atcaagcgtt aaaaaatgag tgggcataaa tgaagcgtta cacacctgac
     4381 tttcctgaaa tgatgcgcct gtgcgagatg aacttttcac aattgcgccg tttgttaccg
     4441 cgcaatgacg cacccggcga aactgtaagc tatcaggtgg caaacgcaca atatcggctg
     4501 acgattgtgg aatcgacccg atacactacc ctggtgacaa tagaacagac tgcgcccgcg
     4561 atcagttact ggagccttcc gtcaatgacg gtgcgtctgt atcatgacgc gatggtggct
     4621 gaagtgtgtt caagccagca gatttttcgc ttcaaagcgc ggtatgatta tcctaataaa
     4681 aagttgcatc aacgcgacga aaagcatcaa attaaccagt ttttagcgga ctggttgcga
     4741 tactgtttag cacatggagc gatggcgatt ccggtttact agcgtcgtga aacctaagga
     4801 caccatttgg aaagcctgtt aacccttcct ctggctggtg aggccagagt caggatttta
     4861 caaattaccg acactcacct gtttgcacaa aagcacgaag ccctgttagg ggtaaacacc
     4921 tgggaaagct accaggcggt gctggaggcg attcgtccac accagcacga attcgacctg
     4981 attgtcgcga caggtgattt agcgcaggat caatcctctg cggcctatca gcatttcgct
     5041 gaaggcatcg caagttttcg tgcgccctgc gtctggctgc cgggcaacca cgatttccag
     5101 cccgcgatgt acagcgcgtt acaggatgcg ggtatctccc cggcgaagcg ggtgtttatt
     5161 ggtgagcaat ggcaaatcct gttgctggat agccaggtgt ttggcgtgcc gcacggtgag
     5221 ctgagcgagt ttcagcttga gtggctggaa cgtaaactgg ccgatgcgcc agaacgccat
     5281 acgttgctgc tgctgcatca tcatccgcta cctgcgggtt gtagttggct tgatcaacac
     5341 agcctgcgta acgcgggcga actggatacc gtgctggcga agtttccgca cgtcaaatac
     5401 ttgctgtgcg gtcatattca tcaggagctg gatctcgact ggaacggtcg ccgcctgctg
     5461 gcaacgccgt cgacctgtgt gcagtttaag ccgcactgtt ccaactttac gctggacacc
     5521 atcgcgcccg gctggcgtac tctcgagtta catgctgatg gcacgctgac caccgaggtg
     5581 catcgcctgg cggacacacg tttccaacct gataccgctt cagaaggcta ctgatgtcta
     5641 cgcttcttta tttacacggt ttcaacagct cgccgcgctc tgcaaaagcg agcttgttaa
     5701 aaaactggct tgcggaacat caccctgacg ttgagatgat catcccgcag ttgccgccgt
     5761 atccttccga tgcggcagaa ctgctggaat ccattgtcct ggaacatggc ggcgattcgc
     5821 tgggtattgt cggttcgtca ctggggggat attacgccac ctggttgtcg caatgtttta
     5881 tgctgcccgc agtggtggta aacccggcgg tacgcccgtt tgaactgctg acggactatc
     5941 tcggtcagaa cgagaacccc tacaccgggc agcaatatgt gctagagtca cgccatattt
     6001 acgatctcaa agtcatgcag attgacccgc tggaagcgcc ggatttgatc tggctgctgc
     6061 aacagacggg agatgaagtg ctggattacc gccaggcggt ggcgtactac gcttcctgcc
     6121 gccagactgt catagaaggc ggcaatcacg cattcacggg cttcgaagat tatttcaacc
     6181 cgatcgtcga ttttcttggt ctgcaccatc tctgacgatc aactcgaatt gctaacttaa
     6241 accatgacgc aaacttataa cgctgatgcc attgaggtac tcaccgggct tgagccagtt
     6301 cgccgccgtc cggggatgta taccgatacc actcgcccta accatttggg gcaagaagtt
     6361 attgataaca gtgtcgatga agcactggcg ggtcacgcaa aacgcgtgga cgtaatctta
     6421 catgctgacc agtcgttaga agtgattgac gatgggcgcg ggatgccggt ggatattcac
     6481 ccggaagagg gtgtaccggc ggttgaactg attctttgcc gtctgcatgc aggcggtaaa
     6541 ttctctaaca aaaattacca gttctctggc ggcctgcatg gcgtggggat ttcggtggtt
     6601 aacgccctgt cgaagcgcgt agaagttaac gtacgccgcg atggtcaggt ttataacatc
     6661 gcctttgaaa atggcgaaaa ggtgcaggat ttacaggttg tcggcacttg cggtaagcgc
     6721 aataccggta ccagcgtgca cttctggccg gatgaaacct tctttgacag cccgcgtttt
     6781 tctgtttcac gcctgacgca tgtgctgaag gccaaagcag tactgtgccc tggtgttgag
     6841 atcaccttta aagatgagat caacaacacc gaacagcgct ggtgctatca ggacggtctg
     6901 aatgattacc tggcggaagc ggtaaacggt ttaccgacgc tgccagaaaa accgtttatc
     6961 ggtaatttcg ctggcgatac tgaagcggtg gactgggcgc tactgtggct accggaaggc
     7021 ggtgaactgc tgaccgaaag ctacgtcaac ctgatcccaa cgatgcaggg cggtacccat
     7081 gttaatggcc tgcgtcaggg cctgctggac gcgatgcgtg aattctgtga ataccgcaac
     7141 attctgccgc gcggtgtaaa gctgtcggcg gaagatatct gggatcgctg cgcctatgtg
     7201 ctgtcagtaa aaatgcagga tccgcagttt gccgggcaga ccaaagaacg tctctcttcg
     7261 cgtcagtgtg cggcatttgt ttctggcgtg gtgaaagatg ccttcacctt gtggctgaac
     7321 cagaacgttc aggcagctga actgctggcg gagatggcga tttccagcgc ccagcgtcgt
     7381 atgcgtgcgg ctaaaaaagt ggtgcgtaaa aagctaacca gcggcccggc gctgcctggt
     7441 aaattggctg actgtaccgc gcaggatctt aaccgtaccg aactattcct tgtggaaggt
     7501 gactccgcag gcggatctgc caagcaggcg cgcgatcgcg aatatcaggc gatcatgccg
     7561 ctgaaaggta agatccttaa cacctgggaa gtctcttccg acgaagtgct ggcttcgcag
     7621 gaagtgcacg atatttcggt agcgatcggt atcgatcctg acagtgacga tctgagccaa
     7681 cttcgttacg gcaagatctg tatcctggcg gatgctgact ccgatggtct gcacattgcc
     7741 acgctgctct gcgctttgtt tgtaaaacac ttccgcgcat tggtgaaaca cggtcacgtt
     7801 tacgttgcac tgccaccgct ctaccgtatt gacctcggga aagaggttta ttacgcgctg
     7861 acggaagaag agaaagaggg cgtacttgag caattaaaac gcaagaaagg caagccaaac
     7921 gtccagcgtt ttaaaggtct cggggaaatg aacccgatgc aattgcgcga aaccacgctt
     7981 gatccgaaca ctcgccgtct ggtgcagttg actatcgatg atgaagacga tcagcgtact
     8041 gacgcgatga tggatatgct gctggcgaag aaacgctcgg aagatcgccg caactggttg
     8101 caagaaaaag gcgacatggc ggagattgag gtctgataat cttaacccgg caaacatgtg
     8161 ttttgccggg ttgtcgttga ttattttatt gggcacagtt tattacgatc tggaaatagt
     8221 gttgccagcg aaccagccag agttttccag tccgtagcca ttttttctgc ttgtgcagtt
     8281 gttattactg caccttgttg tttaataagc tcatcctcca ctctggtata gtgaataatg
     8341 gtaaaatccg tagaggacca tttatcctta gagaacatct tcgtcatcgc atgaatgttt
     8401 gcttgcccgt catcgataaa aacaatcgcg tcatatttgt tccccatttt atccagcata
     8461 taatcgagaa ttacgccttt atcttgcccg gaagaaaaaa ataccccctg gctgtacagc
     8521 catgaccgtt tgagtttccc tttttcaaag ggtggcaaag tatcctcttt cttcctgagt
     8581 gcggaagacg aaaaatcaat gccgtttcgc tttaattccc gcaatgtcgg aaagagagta
     8641 tccggtgcac gggatgtcag agcaaaagcg gtatgctttt gttgccactg ttttaacatt
     8701 gtcggtaact gtggctcggt aagctgcata gggctaagtt cgtaaagcat actgattgta
     8761 ttttcataca ggcaaggaac tttttgcgag tcatccggct ttaaaggcaa tttatcggtt
     8821 tgccattgat accagatatc accgccaatg cgagtgccac tcgttaataa cgtgttatca
     8881 atgtcaaaaa ccaataatat cttatcggat ggatagagag tttccttttc tgtgataatt
     8941 ttactgaccg cactatattc gccagtaact atatttttta cctgtgcgta agataaagca
     9001 ggcagagcag agagcatcat ccagagaatg attttaaatt tattattcat tttttattcc
     9061 agattagaaa gtataagtca gaccccagcg ccagcggatt tggcgattac ttgatgtttt
     9121 atccacacct ttataatccg caatactcat atagggtatc cacgatccca ttttgtaccc
     9181 tactttaaat gatgcggtat agtttttatg gccatcattc caggttaaat aaccattatt
     9241 gtaatagacc aggtttaact gatattggaa gttggttttt tgtggtcgat agtcgatcca
     9301 cacatcgtaa cggcggttat tacgatcatc gttgccataa cctttggatt tatcggtggc
     9361 tttatccatg tcataacgaa accgacctga aactttccat tctttgctga agtcataacc
     9421 cgcagaaaga ccatattcgt aagttgacca atattgcgtg gtatcaagcc agaacatggg
     9481 ttctaaccag aattttttgt catccagcgg cttaaagcgg taaacaacag ataatcctgt
     9541 tccgttattg ccaaattgtg aggggtccca cttatcttta tcaccttcat cggtgttata
     9601 gcgtaattct cccatcaaac cgataccatt actaaaaaat gtaccaaatt caacgcggtc
     9661 cgcgtgggtg cgagtatcat caagatactc atggcgataa tcaataaatc catgcggctg
     9721 ttctgcacag actagtgtag gtgacaggca ggataacatt gttattcctg caagtgatat
     9781 tttaagataa gaattcataa tattcccttt aagaataaaa t
//
LOCUS       DAZGHF010000160         9762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGHF010000160 DAZGHF010000000
VERSION     DAZGHF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.161"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(59..679)
                     /gene="sodA"
                     /locus_tag="ACHK3A_004294"
     CDS             complement(59..679)
                     /gene="sodA"
                     /locus_tag="ACHK3A_004294"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO8742301.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            964..1998
                     /gene="rhaT"
                     /locus_tag="ACHK3A_004295"
     CDS             964..1998
                     /gene="rhaT"
                     /locus_tag="ACHK3A_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO8742302.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(1995..2843)
                     /gene="rhaR"
                     /locus_tag="ACHK3A_004296"
     CDS             complement(1995..2843)
                     /gene="rhaR"
                     /locus_tag="ACHK3A_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO8742303.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQIIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(2917..3753)
                     /gene="rhaS"
                     /locus_tag="ACHK3A_004297"
     CDS             complement(2917..3753)
                     /gene="rhaS"
                     /locus_tag="ACHK3A_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO8742304.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARYLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            4041..5510
                     /gene="rhaB"
                     /locus_tag="ACHK3A_004298"
     CDS             4041..5510
                     /gene="rhaB"
                     /locus_tag="ACHK3A_004298"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO8742305.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTHGLMAQAQRQLGKCDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQSNEIPVVAVASHDTASAVIASPLNSSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            5507..6766
                     /gene="rhaA"
                     /locus_tag="ACHK3A_004299"
     CDS             5507..6766
                     /gene="rhaA"
                     /locus_tag="ACHK3A_004299"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO8742306.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVARDQIKPEHFKNWVEWAKTNQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLESAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
     gene            6951..7775
                     /gene="rhaD"
                     /locus_tag="ACHK3A_004300"
     CDS             6951..7775
                     /gene="rhaD"
                     /locus_tag="ACHK3A_004300"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO8742307.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNKAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            7785..8099
                     /gene="rhaM"
                     /locus_tag="ACHK3A_004301"
     CDS             7785..8099
                     /gene="rhaM"
                     /locus_tag="ACHK3A_004301"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO8742308.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPTNPDNSPVSSELQEVFY
                     LP"
     gene            complement(8140..9534)
                     /locus_tag="ACHK3A_004302"
     CDS             complement(8140..9534)
                     /locus_tag="ACHK3A_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO8742309.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATSESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         2371 a         2497 c         2500 g         2394 t
ORIGIN      
        1 gttcattttt taagctgata tgcggcgtaa acgcctcatt gcagcaggcg gcaaatgatt
       61 attttttcgc cgcgaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
      121 tgatgtagtc aggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
      181 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggggaatcct
      241 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
      301 aaccaaagcg ggaagctgcc gctttctcaa attctgcttt gaagttatca acggagccga
      361 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
      421 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
      481 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
      541 gcaggctttc cagcgccgcg ttggcgttgt tgacgtaggt ctgatggtgt ttggtgtggt
      601 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
      661 atggcagggt ataactcata ttcatctcca gtgttgtcgg gcggccgatt gttaatgccg
      721 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
      781 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
      841 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
      901 gcacgatttt tacactcatc tcgtcggaga tgtgacgcga cgaaaaatga tgaggataag
      961 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     1021 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     1081 gttggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     1141 aatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     1201 ggcgcaatgt gggggattgg taatatcaac tacggcctga ccatgcgtta tctcggcatg
     1261 tcgatgggga ttggtatcgc cattggtatc acgttgattg ttggtacgct gatgacgcca
     1321 attatcaacg gcaatttcga tgtgttgatc aataccgaag gtggacgcat gacattgctc
     1381 ggcgttctgg tggcgctgat tggtgtaggg attgtgactc gcgccgggca gttgaaagag
     1441 cgcaagatgg gcattaaagc cgaagagttc aacctgaaaa aagggctggt gctggcggtg
     1501 atgtgcggca ttttctctgc cggaatgtcc tttgcgatga acgccgcaaa accgatgcat
     1561 gaagccgctg ccgcactggg cgtcgatcca ctgtatgtcg ctctgccaag ctatgttgtc
     1621 atcatgggcg gcggcgcgat cattaacctc ggtttctgct tcattcgtct ggcaaaagtg
     1681 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     1741 ttactctcag cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctggggc
     1801 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagcttctat
     1861 gtattgtgcg gcggtatcgt cgggttggtg ctgaaagagt ggaacaatgc aggtcgccgt
     1921 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgccaa catcgtcggc
     1981 atgggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     2041 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtt
     2101 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     2161 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     2221 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     2281 ctggccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     2341 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactct
     2401 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataatc
     2461 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggca
     2521 ctaaatcccg gaatcgcccc ctgccagtca agattcagct tcagccgctc cgggcaataa
     2581 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     2641 atgtaaaaga gatcgccacg ggtaatgcga taagggcgat cgttgagtac atgcagacca
     2701 ttaccgcgcc agacaattac cagctcacaa aaatcatgtg tatgttcagc aaagacatct
     2761 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     2821 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     2881 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaaac catcccgtcc
     2941 ctgacgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     3001 actgtcgctg aatccgcagc gataggcgat gtcagtaacg ctggcctcgc tgtggcgtag
     3061 cagatatcgg gctttcatca gtcgcaggcg gttcaggtaa cgctgaggcg tcagccccgt
     3121 ttgctgctta agctggcgat gcagcgtacg caatgaaaga gaaaattgat ccgccacggc
     3181 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     3241 tgacgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     3301 cataaataag atctcgcgac tggcggtcga gggtaaatca ttttcctctt cctgctgttc
     3361 catctgtgca accagctgtc gcacctgctg caacacgctg tggttaacgc gccagtgaga
     3421 cggatactgc ccatcctgct cttgtggcag caactgattc agcccggcga gaaactgaaa
     3481 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     3541 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     3601 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     3661 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttaccaga
     3721 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     3781 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacgggaa accacgtaaa
     3841 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacacca
     3901 ccacaattca gcgaattgtg aacatcatca cgttcatctt tccctggttg ccaatgaccc
     3961 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     4021 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     4081 ccagtggacg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     4141 aaatccatcg ttttaacaat gggctgcata gccagaacgg ttatgtcacc tgggatgtgg
     4201 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     4261 tcgatagcat tgggattgat acctggggcg tggactttgt gctgcttgac caacagggtc
     4321 agcgcgtggg cctgcctgtt gcttatcgcg atagccgcac ccatggtctg atggcgcagg
     4381 cacaacggca actcggcaaa tgcgatattt atcaacgtag cggcatccag tttttgccct
     4441 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     4501 ttgctcacgc tctgctgatg ccggattact tcagttatcg tctgaccggc aagatgaact
     4561 gggaatatac caacgccacg accacgcaac tggtcaatat caatagcgac gactgggacg
     4621 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     4681 gtaatgtcat tgggcactgg atttgcccgc agagtaatga gattccggtg gtcgccgttg
     4741 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacagttca cgcgccgctt
     4801 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     4861 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     4921 tgaaaaatat tatgggctta tggctgcttc agcgggtgct tcaggagcgg caaatcaacg
     4981 atcttccggc gcttatcgcc gcgacacagg cacttccggc ttgccgcttc accatcaatc
     5041 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     5101 gggaaacggc gcaaccaatc ccggaaagtg atgctgaact ggcgcgctgt attttcgaca
     5161 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcaactgcgc ggtgaagatt
     5221 tctcacaact gcatattgtc ggcggtggct gccagaacgc gctgctcaac cagttatgtg
     5281 ctgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcgacg ctcggcaata
     5341 tcggcatcca gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     5401 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     5461 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     5521 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggca gtagggattg atgtcgagga
     5581 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     5641 ttccggtttt gaaaacccgg aaggttcgct gaccggtggg attcaggcta ctggtaatta
     5701 tccgggcaaa gcgcgtaatg ccagcgagct acgtgccgat ctggaacaag ctatgcggct
     5761 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     5821 cgcgcgcgac caaatcaaac cagagcactt caaaaactgg gttgaatggg cgaaaaccaa
     5881 tcagctcggt ctggatttta acccctcctg tttttcgcat ccgctaagcg ccgatggctt
     5941 tacgctttcc catgccgacg acagtattcg ccagttctgg attgatcact gcaaggccag
     6001 ccgccgtgtt tcagcctatt ttggcgagca actcggcaca ccgtcggtga tgaacatctg
     6061 gatcccggat ggcatgaaag atatcaccgt tgaccgtctc gccccgcgcc agcgtctgct
     6121 ggcagcactg gatgaggtga tcagcgagaa gctggatccg gcgcatcata tcgatgccgt
     6181 tgagagcaaa ttgtttggca ttggcgcgga gagctacacg gttggctcca atgagtttta
     6241 cctggggtat gccaccagcc gccagacggc gctgtgcctg gacgccgggc atttccaccc
     6301 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtgccgc agttgctgct
     6361 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     6421 ccaggcgatt gccagtgaga ttgttcgtca cgatctgttc gaccgggtgc atatcggcct
     6481 cgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     6541 gaaaaaagcc ctgctacgtg cgttactgga acctaccgct gaactacgca agctggaatc
     6601 ggcgggcgat tacactgcgc gtctggcgct actggaagag cagaaatcgt tgccgtggca
     6661 ggcggtatgg gaaatgtatt gtcagcgtca cgatacgcca gcgggtagcg aatggctgga
     6721 gaacgtgcgg gcttatgaga aagaaatttt gagtcgccgc gggtaaacac tgccggatgc
     6781 ggcacaagtg ccttatccgg cctacgggtc ggcaatagtt gtaggcctga taagacgcga
     6841 cagcgtcgca tcaggcattg attgccggat gcggcacgag tgccttatcc ggcctacaga
     6901 tcgtatatcc ggcaacaggc acggacgtcc aattaacagg aacaccgaac atgcaaaaca
     6961 ttactcagtc ctggtttgtc cagggaatga ttaaagccac caccgacgcc tggctgaaag
     7021 gctgggacga gcgcaacggc ggcaacctga cgctgcgcct ggatgacgcc gatatcgcac
     7081 catatcacga caattttcac caacaaccgc gctatatccc gctcagccag cccatgcctt
     7141 tactggcaaa tacaccgttt attgtcaccg gctcaggcaa attcttccgt aacgtccagc
     7201 ttgatcctgc ggctaactta ggcatcgtaa aagtcgacag cgacggcgcg ggctaccaca
     7261 ttctttgggg gttaaccaac aaagccgtcc ccacttccga acttccggct cactttctct
     7321 cccactgcga gcgcattaaa gccaccaacg gtaaagatcg ggtgatcatg cactgccatg
     7381 ccaccaacct gatcgccctt acctacgtac tggaaaacga caccgcggtc ttcactcgcc
     7441 aactgtggga aggcagcacc gaatgtctgg tagtgttccc ggatggcgtc ggcattttgc
     7501 cgtggatggt gcccggcacc gacgaaatcg gccaggcgac cgcacaggaa atgcaaaaac
     7561 attcgctggt gttgtggccc ttccacggcg tcttcggcag cggtccgaca ctggatgaaa
     7621 cattcggttt aatcgacacc gcagaaaaat cagcacaagt attagtgaag gtttattcga
     7681 tgggcggcat gaaacagacc atcagtcgtg aagagctgat agcgctcggc cagcgtttcg
     7741 gcgttacacc actcgccagt gcgctggcgc tgtaaggagc aaacatgatc cgcaaagcct
     7801 ttgtcatgca ggttaacccc gacgcccacg aagagtatca gcgtcgtcat aatccaatct
     7861 ggccggaact ggaagcagtg ttgaaatctc acggtgcgca taactacgcc atttacctcg
     7921 acaaagcgcg taatctgctg tttgccacgg tagagattga atctgaagaa cggtggaatg
     7981 cggtagccag tactgatgtt tgccaacgtt ggtggaaata tatgaccgac gttatgccaa
     8041 ctaacccgga taacagccca gtcagtagcg agctgcaaga agtgttttac ctgccttaat
     8101 gagttgcggt gccggaaaat atccagcacc gcgacttcat cagaagaagt atttaaagcg
     8161 aacgcgtgca ccgtaacggt catcagtatc atcaccgtgt tttttatcac cgtcgctatc
     8221 cagaatggag taatacgtcc cgaggtaaat attaaagttc tccatatcca tcacattagc
     8281 gaactgataa gacgcatgaa tggtgtgaat ggtgtacgtc ccttcatcat caatccagca
     8341 gtcgttatca caaactacac cgctgaattc atcaatctta ttgtgcgcgt agatataacc
     8401 cagctcgaaa cgtttccaca gcgcgttaat cccggcagtg aaatctttct cattattagc
     8461 atctaaatag gcggtattaa gattaaccac gacaccattt tccggatccg ttttcaggcc
     8521 attccaggtc atgctcatac catagccggt acgatcggac tgatcgacaa aattaccctt
     8581 gctatcggta taaccataag cattatttac cacgttcgct tccatcgctg cggaaacggt
     8641 gaattcttcc gtcggcgacc aggcaattac cggacgcaga taagcaacat ttttctgctg
     8701 ttccatatcg cgtccatgat aattaccgtc gttatataaa gatgttccgt cttccagtaa
     8761 cgtgtttaat tcaaaatacc agttatcgag ttgtttgctg acgaggaaat taccgccagc
     8821 gttagaacgc ccgcggccct ctttcatcat atagatataa ccgctgccat cgtcataaag
     8881 atcgttcgca gtattaccgg aatgttcaac aaaggtatcc tgattcagcg ggaacatatc
     8941 gtaggcttca aaacggccta ctttgacctt ccagtcgttt tctttgccaa agaagaatac
     9001 cgcatcatcg atatttactg aaccgtgcat gtcccccagc ggttgtgcgg agaacccggc
     9061 gaaataaccg ttgtccatct ttcgcatacc gtcaaaacct aacagaatac gaccattgag
     9121 attccactgt tcattagtgg gatcgctatt cacatcagcg ttggtcattg ccagaaggcc
     9181 atgattactt tccgcatcca tattaaattc tacgtcgccg tagattttta aatcgccata
     9241 acctgaaagc aaaagattag gtgaggtgga atttttcttt gcagattccg acgttgcctg
     9301 tccatttacc ggcgtattgc cctgggcagc cttaatttcg cggatctcag cggcttgttg
     9361 tgtctgcagc tgtttaacct ggctctctgc ttttgctgcc cgcatttctg ccgcttccag
     9421 gcgttcttcc agcaatgcca tgcgttgttc cagcgttaaa tgctgtgttt ttgccattcc
     9481 ggtactacat gccaataata aaccgatggc taaagataat gtcgattttt tcattattta
     9541 cgttccctga aataaaagag cccctacgct ttccaaaagg aatacgcata tagagaaata
     9601 taaggtgtgc actttcgaat ttttaatcca acttcagcag gccgatggat agtatcccgc
     9661 catataatat gagtaaatcc gctgatttcc tggaacgtga ggaatataac aatagtgccg
     9721 tttttaacta aatatcgtaa aaaatattag atatgattca cg
//
LOCUS       DAZGHF010000161         9755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGHF010000161 DAZGHF010000000
VERSION     DAZGHF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.162"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..173)
                     /locus_tag="ACHK3A_004303"
     CDS             complement(<1..173)
                     /locus_tag="ACHK3A_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742310.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQ"
     gene            complement(223..525)
                     /locus_tag="ACHK3A_004304"
                     /pseudo
     CDS             complement(223..525)
                     /locus_tag="ACHK3A_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(552..974)
                     /locus_tag="ACHK3A_004305"
     CDS             complement(552..974)
                     /locus_tag="ACHK3A_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8742311.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTEHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKAEATEPVTSKKHSKAS"
     gene            complement(991..1740)
                     /locus_tag="ACHK3A_004306"
     CDS             complement(991..1740)
                     /locus_tag="ACHK3A_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8742312.1"
                     /translation="MTLPNPLAPVKGSGTTLWVYTGNGDAWANPLSDDDWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKSVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTIIVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            complement(1748..2146)
                     /gene="gpU"
                     /locus_tag="ACHK3A_004307"
     CDS             complement(1748..2146)
                     /gene="gpU"
                     /locus_tag="ACHK3A_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO8742313.1"
                     /translation="MKHREIRAAVLSALKENISERVNWFDGRPVFIDEQELPAVAVYL
                     TDASATEEFVDEGTWEAVLHIEVFLRAKETDSVLDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEYMMWGSADLQWNITYQM"
     gene            complement(2159..2782)
                     /locus_tag="ACHK3A_004308"
     CDS             complement(2159..2782)
                     /locus_tag="ACHK3A_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8742314.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWAPSSSGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMTAMAHQLRLVLKRK"
     gene            complement(2785..3066)
                     /locus_tag="ACHK3A_004309"
     CDS             complement(2785..3066)
                     /locus_tag="ACHK3A_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO8742315.1"
                     /translation="MSDLFTRMCCRMDGATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(3059..3385)
                     /locus_tag="ACHK3A_004310"
     CDS             complement(3059..3385)
                     /locus_tag="ACHK3A_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO8742316.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     DAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(3473..5452)
                     /locus_tag="ACHK3A_004311"
     CDS             complement(3473..5452)
                     /locus_tag="ACHK3A_004311"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO8742317.1"
                     /translation="MKNKSWFRMQAGGPGDADIYIYDEIGFWGVTAKQFVSELNALGD
                     ITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPPRNSAPRDTTVT
                     IPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMFGGRYQTLQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKD
                     NAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQG
                     WEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALA
                     TYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFH
                     ADHKNIATGGISVSGLDAARQMMRLQKEGERALNIRPAFMLVPVALETVANQTIKSAS
                     VKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYI
                     DQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(5442..6944)
                     /locus_tag="ACHK3A_004312"
     CDS             complement(5442..6944)
                     /locus_tag="ACHK3A_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8742318.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     VAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(6944..7156)
                     /locus_tag="ACHK3A_004313"
     CDS             complement(6944..7156)
                     /locus_tag="ACHK3A_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742319.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(7153..9276)
                     /locus_tag="ACHK3A_004314"
     CDS             complement(7153..9276)
                     /locus_tag="ACHK3A_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO8742320.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVCKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQARSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            complement(9273..9749)
                     /locus_tag="ACHK3A_004315"
     CDS             complement(9273..9749)
                     /locus_tag="ACHK3A_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO8742321.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGLLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         2061 a         3066 c         2357 g         2271 t
ORIGIN      
        1 ctctgctcag cagcatcagc ctgttttctg aaagactgac tgactttccc ggacatcaaa
       61 tcagcaagcg cactggtttc acccagctct ttttttaccc gcgctgcctc ttcagaaaaa
      121 cgggcagaat ccagtgtcag cacggctgtc agatcggcaa aattacctgc catcgtggtc
      181 acctcctgaa atgtcctctg atgccatcaa taactgtcac aacctccttc cctcctcccc
      241 gaaacggact ccaccggcga ggcccgccgc cttctgcatc agtacatcat tttcgtccgg
      301 cgtctccgcc tgcccttcct ctgcctccgg agcgaacagg ctgaaatccg ccggatgcat
      361 atccggatcg caaaaaaaca ggctgagtac ggcgtacgtc agcccggaaa aatgcatatc
      421 cagctgggta tcctgaaaat aatgcgtgca gtaaaaacgt cgccagtcgg catattcggt
      481 ggatgtcatc ccggcaagca tggcgcgcca gtcggctctc cccatctcac gcgccagttt
      541 caggacaaag ttcagctcgc cttcgaatgc ttttttgatg ttaccggctc agtcgcttct
      601 gctttcccgg ttggttcagg atcggcatcg tgccggttat ccagcatacc tgaaagataa
      661 agcacccggt tcgttgcctg attcagtgca tcagcaggcc atcccagcat cacttcacgg
      721 cggatctgct gcatctctgt ctccggagat gccagagtgc ctttgaggga atgggaatgc
      781 catagcgaca tcgccacaag cagggatgcc gtttccagat atcgctggtt aatgtgcacg
      841 gcatcatgct ccgttgtctc ctgttgtgct gcgtctgaaa caaacttcat ataatcaaac
      901 cgctgcagcg cagacagctc cgacagcgtg acggacaccc cgttatattc aaattgttct
      961 gttttcagga acatgcttga tctcctcccc tcagcccgca gcgccatccg tgacggtgat
     1021 ctccgccacc gccgcaaact caccgttgcc ggtgacaaca gggatctgcg ctttacctgc
     1081 cgcaacacct ttcacgatga tcgtgttccc tttcacggta atggtggcga aattctgatt
     1141 cgctgaggtt gcgcggaacg ttttatccgt tgcgccttcc ggctgaacgg ccacggtcag
     1201 ggtgatattc tgaccttttg ccacattccc cgttagtggc gtcacggtaa taccggtgac
     1261 cggtgtgatt tctccccgat cctcagccag cgacggacga ccgatattgg tgattttcac
     1321 agtgcgggtg atcacttctt tggcggtcac ggatttacca atggcgctca cccagccacg
     1381 gaacacatcc accgtgccgt tcgggaagcg gattttgtag gcccgggtct cgctgctgtc
     1441 aaaccaggca atcaaatcac gctgcccttt ctcgccgggc ttccaggcca gcgtaaaact
     1501 ggtgtcaccg gcagatttct gcccctgccc ggtggatacc cagtcagcat cctcatcatc
     1561 cagatagtta tcgtcgtagg attccgccgt catctcgccg ggggtcagat cctttatttt
     1621 tgccaggcgc gtccagtcat catctgacag cgggtttgcc caggcatcgc catttccggt
     1681 ataaacccag agcgtggtgc cggaaccttt taccggcgca aggggattag ggagcgtcat
     1741 aacgtcctca catctggtaa gtaatattcc actggagatc agccgatccc cacatcatat
     1801 attcgtcatc ccggcgatag tcatacccct gaagattcat cttcagcagt aatgcactta
     1861 gcccgggaac cgcctccagc gccggaagta ttttttcttc catccacata tccagcacgg
     1921 agtccgtctc tttcgccctg agaaacacct caatatgaag cacggcctcc caggtcccct
     1981 cgtcaacaaa ctcctccgtc gcagaagcat ctgtcagata aaccgccacc gcaggcagtt
     2041 cctgttcatc aataaatacc gggcggccgt caaaccagtt cacccgctca gaaatatttt
     2101 ctttcagggc agacagaact gccgcccgta tttcacggtg tttcatacac cctccctgtc
     2161 attttcgttt cagcaccagg cgtaactgat gcgccatggc cgtcatcatc tgcgccggta
     2221 atttttcccg gtacatccgg tcccgttcac gttcaaaggt ttctgccagc ggtccggcag
     2281 tcggaatctt caccacttcg atcggcagac ggtggcgttt cggcctccct ttgctgtccg
     2341 cgccgctgga cgatggtgcc cacggcatac gctgcatcac atgccagcgt ccgttagcca
     2401 gccgggtgat aaaggcgtcc gggatccgtc ttttccccac aatcagcaca ctgccgcccc
     2461 ctttcagggc cgcacgctgt cctttctttc tccgttttct gcgggaaagt cgaacgcggg
     2521 cctcccccag tttgatggcg ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct
     2581 tatccggtcg tgccggactt aaccggatgc gggcacggat aagacgacgg ggaacggcca
     2641 gctccctggc aactgaagag gccgttttcg caataatggc ccccgccacg cggttcagtg
     2701 tcgtggcaga ggcccgggga acggcacggc ggtcaattgc atccagattt ttcatggcct
     2761 gcgccagacc ttttattgcc atactcattc ctgttcgaca aaaatccggg gtttaccgtt
     2821 gtacgtgtca taacgggtca ccgtcagtgt gcgccccgca aacacaacaa catcatgacg
     2881 ggccggacgg taccgggctg aaaacaccac cagtgacaac tggctgcccg aaagcgcccc
     2941 catctccgcg gactcttcct ccggcatcac gtcatacact acgccgttaa tctccgcctg
     3001 tttgcccatc acccgaacgg tcgccccgtc catccggcaa cacattcgcg taaacagatc
     3061 agacattgat ttttaccgcc acagtggcgc tgtttgcagg agcattttcc caggctaccc
     3121 ccgcagccac cgcaccgtct gcagccagct gcacaacccc gtccttcaga taaaccaccg
     3181 cgccggactg aatatcgtca gcagactgtt tgggcagcag gaacacgcct tcggcaaaac
     3241 cgtcaccggc atcaccggca ggaatatcgg taatggccac tgccaccata ctgccgacca
     3301 ccaccgcagc accgctcagg atggtctgat ctccggcatt caccagttca atggtggtgc
     3361 cgtcctgtac aaaatttttc gccataatgc tgtttctccg gacagcccct gtggggctgt
     3421 ttttcaggca taaaaaaagc cctttcgggc agtgattgtg ataacgcggt tatcaggcca
     3481 ccgacgaacg caccagaccg cgccagtcaa gtggtgccac accggcatca atacgaattt
     3541 ttgtggcaat gccgtcagtg gtgaaacctt cctgctgatc aatgtatggc gtgtccacac
     3601 catccagcca ggccacttca atggtgtcag tgccctgtgc cgccgccaga taccaggttt
     3661 tcggatctgc cgcatcaaga cgcgcttccg caatcacctc agcaaagttc tggatggggt
     3721 taatgacacc ggcgtttgca tccgcccctt tcacactggc cgatttgatg gtctggttcg
     3781 ccaccgtctc cagtgccacc ggtaccagca taaaggccgg acggatattc agggcgcgct
     3841 cgccttcttt ctgcaggcgc atcatctgac gggccgcatc cagtccggaa acggagatcc
     3901 cgccggtggc aatatttttg tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg
     3961 ggttatccgt cagcaccttg taaaccaggt caccaatcgt tgccttcgcc gcacgcccca
     4021 tcttcatcgg cacgtccacc agcatattca gatcatcatt gataatggcc tggcgggtga
     4081 tggagaaaat ctccccgtag gtggccagag caatggtctc cttgcgatct gaggtggtga
     4141 tgtatttata ctccgccccc tcacgaacct gacgcaaaga accaaaaccg cccatcccca
     4201 cgcgatacgc tgttttgaag tctgacaggc gtcccttacg ggtccacttc tggaaggttt
     4261 cttctgattc ctcccagccc tggatcagcc ccttgttcga cacatccagc aggatattgc
     4321 caaaatcaga ggtgctgtgc gtaagcgcca gcccgaccat ctgcatgggg ttataactgg
     4381 ccaccccaat accgcgctcc gtcagtgaca tacgagccca ttcacgcagg gtcatcccgt
     4441 tataggcgtt atccttctcg acattttcaa atccggcacg ggccagcatc gcctggcgga
     4501 tcccgtctcc cacaaaattg ccgtttccgg cataaatatg ggccggtgtg tttttgttgg
     4561 tcggcgagga ctccttgccc atttcattca gcagacgttc acgggccatt tccagcgaac
     4621 agtcaggatc agccacgcac tgtgcctgaa gcgtctgata gcgaccgccg aacatggcaa
     4681 acagatcgtt aatgcctgac atgcgtgctt tctgctcagc cataacgcgg gcgcgaatgg
     4741 tcgcctcatc agacactgcc ggtaccggtg atggttctgt taccgccggt gcagggattg
     4801 tcactgtggt atcacgcggg gcactgttgc gtggtggagt aatcatgttt cggatggatt
     4861 ccggcatctt tttaaattcc tctgtacgtt ttgactgaat acatgccatt gcctcaacag
     4921 cgggtgtcac ctggtcagca aatccgtgtg ccagacattc ggcaccggac atccaggttt
     4981 catccgccag catggcggca atttcatcgg tggtttttcc ggttttctgc gcataggctg
     5041 gcaacagtac cgattcgact ttatccagca aatcagcata actgcgcata tcctcagcat
     5101 ccccgccact gaatccccat ggcttatgga tcatcatgaa ggcattttcc ggcataatga
     5161 ccgtatcacc ggccatcgca atcacagatg ccatcgaggc ggcaacgcca tccacataca
     5221 cggtaatggt cgccccctga tttttcaggg cattaaaaat ggcgatgcct tcaaagacat
     5281 cgccacccgg tgagttgatg tggagattaa tgtgggtgat atcacccaga gcattcagtt
     5341 cgctgacaaa ctgcttcgcg gtaactcccc agaaaccaat ctcgtcataa atataaatat
     5401 ccgcgtcacc cggcccccca gcctgcatcc tgaaccagga tttattcttc atgctggctt
     5461 tcggtgtcac gctgatactg tcgttcagtt ccggcactgt tgcctccttt gtcgttgacg
     5521 gggtcagtat caaagaccag ccccagtctg ctgttttcat caatttcagc cttgcggcga
     5581 cgtttgacct catccggatt gcgcccgccg gcacgcaccc agtcagattc tgtcgctgca
     5641 ccaccccgga tctgaattct ccaggcttca gcttccttaa ccgggtcgat ccacggcatc
     5701 accggaccgg aatacgtcgc gttatatagc gttttcatct ccacatccgc cggaattttc
     5761 agcagacctg ccgcaaccac catattcagc catgtccggt acaccgggcg ggttaccgcg
     5821 ccaataaaac agtcctgcag gatcaggtaa ccatccgtgg actcgaccag ctcctgccgc
     5881 tgggcgctgt aggtgccgtt atagttacgc gccgcactgg aaaaactcag acgactgcca
     5941 gctgccactg cacgcaactg gccgttgcgg aaagtttcaa ggttgggatt gggacggtca
     6001 gatttgacca tgccgatatc ctcgcccttg cgcaaatcgt cataaataat acccggggtg
     6061 atatggactt cccgctcggt ttctttgatc cccggatctt catagtcctg tccgtcacct
     6121 ttacggatat acagtcccag cgccgcagca atacgcgccg ctgtcagttc cgcatcctca
     6181 tactctttaa gggcactgat ccgcatcagc acccccgata acatggatga gcctcgcgtc
     6241 tgatgcagac gacgagtgaa cttcaggtgg atcatttttc cggcagcgat ttctttcgta
     6301 tcactctgcc ggccgctgac cggataattt ttataaacca gatatttttt cggtcttccc
     6361 cactcatcaa gaaaaacccc ctgattcagc ccggcggatt catcagtgcg catgggaaca
     6421 aaatccggct ccatcgcctc aagccagaat ggcactcccg ccgtccgttc cagaccgttt
     6481 cccgcaccac tgaccatctg cgcaaacact tcaccatccc gcagccaggt ccgcagcagt
     6541 aaacgttcaa gcacgggacg ggtatactgc cctgtcacat ccggactcac ggaccattca
     6601 gcccacaacc ggcggatatc cgcagccagc tctgccacca tttccccgtt ttttcgtaat
     6661 ggctgaggct ccacaataat tcccctggca ccaatcaccc gctcttccag cttgtcaaac
     6721 acaccaatca ccaggtcatg attgatatcc agaaaacggg cctgctcccg cagggaaacc
     6781 gcaccgtatt tactgagctg atcagcagag cgattttccc gccgggcttt atgtgtccgg
     6841 gtcggtttca ccgcctcata ggccatgatt aacgcccttg aacgcagtct ggctgctttc
     6901 catccggggg aaaacacgcc tatcacatca tcaataattg ccattaaaac ctcgccagtt
     6961 taaatcccgg ttttccccgc ctgcggctca ccatcgcggc aagcctgcgt tcccactcct
     7021 gacgtccggc gcggatctga gaaaggcttt ccagcgtcag ttgctgcccg ttgaaggtga
     7081 cagacttccc ctccagtacg gccatttccg cttcacggta ccgctgtatc atttctctgg
     7141 cttcttctgt gctcacaacc agcctcctga tgttatccat ggattatctt ccgcacgctc
     7201 cgtccgcagt tttttcttcc ggcgacggcg tttttctgcc ccggccgtca gttccgggga
     7261 taccgtttca ccagaacgcg cctgcgggaa gacgagccac gtttcccgct gtgcccagtc
     7321 cggtgcggag ggccagcgga tcttttcata accatgcaga acagcaagcg catccgcata
     7381 aaccagcagg tcaaacgcct cgttagcgcc cctgcccggt tttcgccatt ttccgtcact
     7441 gccgcgctct tcataggtca gctcatcgta aaaccaccgc cccagccagt cgggaaagtg
     7501 gatatagttc ggccctggtg tgtcacgcca cagggcatta tttacacggt ccttaaacgc
     7561 atccgtctga accagccaca gcgcgacatc gccactggct ctggcacggc gggcacttct
     7621 gccggtatta tccgggaagg tacggttaat cagcctgtca cggcgaagac catccccctt
     7681 gaacagaaac accctgttgc ccagtccgtc actccggcaa cgacgccaga aacgataggc
     7741 gttatctgtc accccggctt cccctcccgt atccaccgcc atggccatca gacgcatgcg
     7801 cacatccgga tcagaagcca gcggccatgt tttatggaac acatccgtca gcaacaaatc
     7861 ccagtcctcc ggatatgccg ccggatcaac cggcagactt tcaccgttgg gactgcatcg
     7921 cagtgaatgc cggatgttgt agcgatcaac aatccagcgt tccccctgct ctccgtatcc
     7981 ggtgatctgc acaacaaaac ggcgattttt accgccctgt acgtcaaccg ttgcctcaat
     8041 aaaacgcaca ccatccggca cagatcgccg gggaaacggc tcggcacgct gttcaagcag
     8101 ttcactttta cgctgttccg tggctgaacg gggcagatag ggtcgtccga tatcggtgtt
     8161 caccaccgct ttcagggtct cttcactgcc ggttcgctca tactcttctt ctgccgccag
     8221 cagcttaaaa atcagttgtt cccaggtctg aaacgccgca gccggcccct ccatccagaa
     8281 acttgctatt ctggaatttc ggggggttcc ggtgatactg ccatccgccg ctgcccgttc
     8341 gccttcccgc agccagatcc cctggttatt cagttcacgc ttctgttccg gagcaatcag
     8401 gccgcggcag tgcggacaca tcaggcgggc cgcctgcccg gcagccacaa aatcagggtt
     8461 attccggtaa ccggtcatgt tatccatcac cggctgaaaa tattccccgc agtgcggaca
     8521 cggccagtac caccggcggc ggtctccccg gttatacagt gacaggatcc ccgttgttgg
     8581 cggtgcctca tgtgcgccac cacaacgcca tttggtatcg gtgatatccc gcccgggcga
     8641 actttcgacc agggtcatcc ccgaggacat aaaggtggtg gtacgctttg aggccagggt
     8701 gaaggcatcc ccttccccgt ccacgttttc agggaaacgg tcataatccg tcagcgccac
     8761 acgacggtaa tccgaggagg aaaatacggt gatcgacggc cagccaattt tcaggaatga
     8821 gccgtcaaga aacattttat cgtggacgtt gttgtcatta cgggaagggc tgaggcgttt
     8881 gcagacctcc gggctgtggc gaaacgtcct ggaaagacgc gttctggaat gctcacgcgc
     8941 cttcgtctcg gtcatctgca ccaccagcat gtcagccgga tcacagatga tgccgtacac
     9001 aatccagcca tcaatcagcc cttcggtttt cccggttcgc gccggtccga caaacaccac
     9061 cgcgtcatat tcacgggctg ataatgtatt aatggggtct atcatatagg gcgtcagcga
     9121 tgactcccac ggaccggaag tattggctcc ccgtggtacc cgcatataac gcctgatggc
     9181 ttccgctact ggtaaccggc caggtgggcg aaacagcgag gccacttcgc gccagatatc
     9241 ggatgcgcgg ctatggctct cgttcacctg attcacatat cggcctcatc acaacagtca
     9301 atgactgcct tttccagtgt gtcgcggatc tcatcaacca caatctgtac ttcattcagt
     9361 tgtgatgcgg tccaccccct gtccctctcc agccggtcag gccaggtttc cagtacctga
     9421 actatcgctt tcaccacgac agaaaacgac cgcctgacat cactgacagg cacgagcaga
     9481 ccggtttcct gctcaaattt cagtcggtca cgttctgact ggtaccatgc tttacgcgca
     9541 tgaggatcca tttcctcgtt atctacaggc agaggagctt tcatcagttc ggcaaggata
     9601 tctgtcagtc ggtacagttt gagattgctc tcatgaccac cggctgggct tatgtttttt
     9661 acccgagccg cgacagtctg tcgatgagca ccggataatg cggccagttg ggaaatattc
     9721 agatgcagat tttttaattc acgatccata actcc
//
LOCUS       DAZGHF010000162         9643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGHF010000162 DAZGHF010000000
VERSION     DAZGHF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.163"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(580..2184)
                     /locus_tag="ACHK3A_004316"
     CDS             complement(580..2184)
                     /locus_tag="ACHK3A_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO8742322.1"
                     /translation="MNPDYVQALQTSPHFPTDVTYRLFPSDLAYLIDDLYSSTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMTPCYE
                     FADRLIQQYEERNKDYKTELQIWNTRQKALAANLRKAVNRGDPGEQEEEALRNHERNK
                     PTRPVRPNFIYEDVSLKALAEGLNEHPEAGIISDEAVTFFRSYLKNNPGLLNKAWSGQ
                     PFDFGRADEKYHITPRLTFSLMSQPNVFTDYINKNDVLAWGSGFLSRFLFSQVGGSSR
                     VRDYRKGEFVTIPTLVEFHKKINGFLSSHSINSPGMRSERKTLKLSKKALGEWQENQI
                     KIESKALAGGEWEHIRDIVLKAGSNILRIAGLFTCYCYKDDEEITSIALWKAMHLMEW
                     YLEEASTIFYPMSERCQFEQDARELYTWIMTRIKQNNLYAIRKTDIERYGPNRLRRAE
                     KLTPVLNQLIAQNYLCIIKMRSHQALYVSVHCNNRYLLPCGAMSYEQFDIVPPLNSHN
                     AKTYHVNIPLALIPSFALPFSAYTLF"
     gene            complement(2614..3519)
                     /locus_tag="ACHK3A_004317"
     CDS             complement(2614..3519)
                     /locus_tag="ACHK3A_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8742323.1"
                     /translation="MALEQEIANLVNDFVAGGRPSCRDQTFDERRAGYIASTALAGDT
                     ETRVNISDVIIDDILLRIFSPLNDDKLSSAIVYYHGGCFVSGGFVTHDNQFRQLAFLS
                     GCRVIAVQYRLAPEHIYPAAHDDAEHAANIVWQHAEMLKVDRNKITLMGDSAGGHLAL
                     ITALRVKNIAQWLPRKLILIYPMLDATASCDSYKLNGSDYVLTEDTFLSGYEAYFSGL
                     DLEHPEASPLFRKDFNGLPPVHIITAEYDPLRDEGEELFSRMIQQGVKCTAQRYLGVI
                     HGFFQLGGISQTARNLMQDICGIVE"
     gene            complement(3973..4425)
                     /locus_tag="ACHK3A_004318"
     CDS             complement(3973..4425)
                     /locus_tag="ACHK3A_004318"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO8742324.1"
                     /translation="MNVIDSGIRDVKFGENVKVVMPVNLYECELGDDVFIGPFVEIQK
                     GCIIGNRSRIQSHSFICENVILGDGCFIGHGVTFANDLFKCGGPDASAESWIRIVLGN
                     TVTVGSGATILTTKICSGAVIGAGSVVTKPVLVKGVYAGNPARLLRTL"
     gene            complement(4939..5670)
                     /locus_tag="ACHK3A_004319"
     CDS             complement(4939..5670)
                     /locus_tag="ACHK3A_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO8742325.1"
                     /translation="MNQSIHNAYWLSRFDSLLDSAPAQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDIWCSLGDFSEPSSLATMIQQAWCSALHPEPWQGDGLVHFSRWTPSRKPA
                     AADASPSSDDTPLHSGCSDTRTDSDKRPGGSAVLWAKRGDVEAMQKALERARYLVKNE
                     TKLHDGSGQRNYGCSRGVRCLLDGR"
     gene            6341..6871
                     /locus_tag="ACHK3A_004320"
     CDS             6341..6871
                     /locus_tag="ACHK3A_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8742326.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAAMTITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
     gene            8551..9354
                     /locus_tag="ACHK3A_004321"
     CDS             8551..9354
                     /locus_tag="ACHK3A_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742327.1"
                     /translation="MSADGISDLIKPEIFLVIFPGTTTTAKRGGAGWLNNKRVVFRVW
                     LYISKASYKKMGIFQRGSAPESIPYREYHFPSYPDTHEKRKKVLRDWISFMRSRFNIN
                     QIAQFCYRMENEWAFTFDNIHVPRHFREGTDDTEWSWNYIKKKRLFSLSGLQHIQPQN
                     NTERKLLINAFFDTCLIENNPADDIDIKNMLLRKMNAAFYKRKSRARARIHSRNNQQM
                     SVSSGTDKQVNIRLKDFQYQQLLFIAQKENKSKSEIIKELITRAYRAIQ"
     gene            complement(9411..>9643)
                     /locus_tag="ACHK3A_004322"
     CDS             complement(9411..>9643)
                     /locus_tag="ACHK3A_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742328.1"
                     /translation="PVENDMSLSEELAHVLFFTLLHSDIMSRFSLRELFFRIESGRDN
                     NKKRDLIREYVSDVTKEYSLCIPGIVSRHDKK"
BASE COUNT         2946 a         2184 c         1857 g         2656 t
ORIGIN      
        1 taaattaaaa ttatccttgt ttgatgagtc aaaatgagga attatccatt ctggatatga
       61 gtctgacaaa acaattctct tacagaaaaa tatcaaatcg tttttcttca atcccctatt
      121 gaaagctaaa caacatctct tatcagtaac agactgataa cctttcgtta ttcagaaaaa
      181 gatcgacatc gtgatatacg ccagcactca tcacgacaac aatcaaacaa agttatatat
      241 aaaaactcac ataatatggt taataattag cttcaatcca tgtgaaaaat aacatttcga
      301 ttcactttcg agatatcaaa aacgaaaaca attcttccac cagtgcacac attcctgcat
      361 tcaaccagct ctgacaacag gtcattatga aaacaaaatc aaatcacata catggtacat
      421 gtactgctac cgttgctacc gtagcgattt tttcagagaa attttcgttc acaccccata
      481 attggtgaat atttgcacaa atgcaggata tgaaaaacgc cttaccatca accttcgaat
      541 gaaggcttat gtgttttgtt tgcttattgt aaagctgttt caaaataagg tataggctga
      601 aaaaggtaac gcaaatgacg gaattaatgc cagagggata ttaacgtgat atgttttcgc
      661 attatgacta ttcaagggtg gaacaatatc aaactgttcg taagacattg caccgcaagg
      721 aaggaggtaa cgattgttac aatgaacaga aacataaagt gcctgatgcg atcgcatttt
      781 gatgatacat aaataattct gtgcgattaa ctgattgagt acaggtgtaa gtttttctgc
      841 tcttctcaga cgattggggc catatctctc aatgtctgtt ttcctgatag catacaaatt
      901 attctgcttg attcgggtca taatccatgt atacagttca cgggcatcct gttcaaactg
      961 gcatcgttca gacataggat aaaatattgt gcttgcctcc tccagatacc actccatgag
     1021 atgcatcgct ttccaaagcg ctattgatgt aatttcctca tcgtctttat agcaatagca
     1081 ggtgaataaa cccgctatcc tcagtatatt agaacctgct ttcagaacaa tatctctgat
     1141 gtgttcccat tccccacctg caagcgcttt gctttcaatt ttaatctggt tttcctgcca
     1201 ctctcccaac gctttcttcg aaagttttaa tgtcttcctt tcgcttctca ttccggggga
     1261 attaatgcta tggcttgaca gaaacccgtt aatcttttta tgaaactcca ccagggttgg
     1321 tattgttacg aactcgcctt tcctataatc acgtacccgg gaagagcctc caacctgact
     1381 gaaaagaaaa cgggaaagaa atccgcttcc ccatgccagt acatcatttt tatttatata
     1441 atccgtaaaa acattcggct gagacattaa tgaaaatgtc aggcgtggag taatatgata
     1501 tttttcgtct gctctcccaa aatcaaacgg ttgtccactc catgctttat tcaacagacc
     1561 cggattattt ttcagatagc ttctgaaaaa agttaccgct tcatcagaaa taatccctgc
     1621 ctccggatgt tcgttgagtc cttccgcaag cgctttaagc gaaacatctt catagataaa
     1681 attcggtcga accggacgtg tcggtttatt tctttcgtga ttacgcagtg cctcttcttc
     1741 ctgttctccc ggatcccccc ggttaacagc ctttcttaaa ttggcagcaa gagctttctg
     1801 ccgggtattc cagatctgta gttcagtctt ataatctttg tttctctctt cgtattgttg
     1861 aatgagtcga tcggcaaatt cgtaacaggg ggtcatcacc agtctgttta tcgttgtttt
     1921 tcccgcgcct ggctctgcga ttgccaacag ataaagtgag cagggttccg gcatgttagt
     1981 gtgaggatga acaacgtcaa ccaatgactg acaggagagt gagagcgttg ccagtacagt
     2041 attaaaaatg agctccagag gaagttgggt acttgaatac aagtcgtcaa tgagatacgc
     2101 aagatcacta ggaaataatc tgtaggttac atctgttggg aagtgggggg atgtctgcaa
     2161 ggcttgcaca taatcgggat tcattgtaat ggacctcctt cacatactgt aatcagcaca
     2221 aagcttattg ttcagttcag aaacatacca acattgtgag tatgttcgct gacgccaata
     2281 ataaaatgac tgatctccat tcaccagtcc cggcgcattt atggttctga caaaagtaaa
     2341 caacatggga gccagaactc tgcatcgcag cgacaaacta tggatgcatg aaaaacaggg
     2401 aacattacgc accagagcgt tgagagtctt cctccttgcc caatacgaaa gtcatctatc
     2461 cacccgtctt caatcttcgg gatggttgtt ttatgaacag agtaaaagtt tgaagcaagc
     2521 tgagaacctc tgtttccacg taaaggtccg ctatgaagaa aaatgggagt ttgcatgctc
     2581 aactaattta cctgtaggtg cttgttactg tagttattca acaatcccac aaatatcctg
     2641 catcagatta cgggctgtct ggctaatgcc gccaagctgg aaaaaaccgt gaatgacacc
     2701 gaggtaacgc tgggcggtac actttactcc ttgttgaatc atacgggaaa acagttcctc
     2761 accttcatcg cgtaatgggt catattccgc tgtgatgata tgaacaggtg gtaacccatt
     2821 gaaatctttt ctgaataatg gactggcttc cggatgttct aaatccagac cggaaaaata
     2881 agcctcataa ccactaagaa acgtgtcttc tgtcagcaca taatcacttc cgtttaattt
     2941 gtaactatcg catgatgctg ttgcatccag catagggtaa ataagtatta attttcgtgg
     3001 taaccactga gcgatgtttt tcacccgcag tgctgttatc agtgcaagat gtccccctgc
     3061 gctatctccc attaacgtta ttttgttgcg atcaactttc aacatttctg cgtgttgcca
     3121 tacaatatta gcggcatgtt cagcatcgtc atgtgccgca ggataaatat gttccggcgc
     3181 taatcgatat tgtacagcta tgacacgaca gccgctcagg aaagcaagct gtcgaaactg
     3241 attatcatgg gttacaaaac caccactgac aaaacagcca ccatgataat agacaattgc
     3301 ggatgacaat ttatcatcgt ttaaaggcga aaagatacgc aataaaatat catcaataat
     3361 cacatcactg atgtttacgc gggtttctgt atcgccggcc agagcagtac ttgcaatata
     3421 tcccgcccga cgttcatcaa atgtctgatc acgacaggat ggacgaccac ccgcaacaaa
     3481 atcatttaca aggtttgcga tttcctgttc aagtgccata caaatcaact ccgaccgata
     3541 atatttaaac aacgctacgg gaagtataaa tactctgacc aaaatataac aggtgaatat
     3601 ccatagcttc aacaggcgta atcaataagg gcgtacggag cttgcccgtt attgctgcag
     3661 gatcgacaga ctggttttcg tccttgcgca tgtaacagta caaccacaga gaaaagcatc
     3721 aatggacact ttatgttgcg tcagggcagc gaacgactcc ttttgttgac gacacatatc
     3781 accttctgta ctggcaatta cggcattcag agtctccctg agttttaagc tcctgctctt
     3841 acggaccagc gacaagcaat aaataactat gacaatgaat ctaataacag cataaatatg
     3901 atactcatcg tgattagtat aatcggggca gaattatatt cactgtctga tttattattg
     3961 ggttaaatat aattaaagtg ttctcaaaag tctggctgga ttaccggcat acacgccttt
     4021 taccagcacg ggctttgtaa ccacacttcc agcaccaatc actgctccac tgcaaatttt
     4081 tgttgttaaa atagtcgctc cgctacccac tgttacagtg ttacctaata cgatacgtat
     4141 ccagctttct gcagaggcat cgggaccacc acacttaaaa agatcattag caaaagttac
     4201 gccatggccg atgaaacagc catcacctaa aatgacgttc tcacagatga aactgtggga
     4261 ctgaattcgg ctccggttgc caatgatgca tcccttctgt atttccacaa agggaccaat
     4321 aaaaacatca tcccccagtt cacattcata gaggtttact ggcataacga ctttcacatt
     4381 ttcaccaaat ttcacatcac gaatgccgct gtcaataacg ttcatatccg ttgtatcctc
     4441 cctcaggatg attcttatca gaacatttca cctgtcagaa tattcccggt aatgtgatac
     4501 aagatccagt ctcaccgggt accacgtgtt attgcccgta tccttttgcc cgttacgagt
     4561 ctacaactgg ttttgtattt tgcaatccag gcctgttaca ccagagcatc ctctcacttc
     4621 cccctcactg gtcagtccgg gattcaacat catcctccag cactaaccct ataccagtcc
     4681 tgaaccttcc ctcgccagca gcctactgtt caccacaatc ctcttccgtg aatggcagta
     4741 agtgagctgc accaaaataa atcggtactt ccgatgacag acagtggaag tgccaggaga
     4801 aatatgaaca tgagaatgga agcagataaa aataccgttg catgacttca ggacgggtta
     4861 tcagaatacg agctaagggc gtcagccaac gcccttaccg tggtatacac tcaggcaccg
     4921 gataccattt taccgggttc acctgccatc cagtagacac ctcaccccac gactgcaacc
     4981 ataattacgt tgacctgaac cgtcatgcag cttcgtttca tttttcacga gataacgggc
     5041 tctctccagg gctttctgca ttgcttccac atcaccacgc ttggcccaga gcacagcgga
     5101 tccacccggc cttttatcag aatccgtccg ggtatcagaa catccactat gcaaaggcgt
     5161 gtcatcggaa gaaggggaag catcagccgc tgctggttta cgggaaggcg tccacctgga
     5221 aaaatggacc aggccatcgc cttgccaggg ctcaggatgc agagcgctgc accatgcctg
     5281 ttggatcatc gtcgccagcg aagacggttc gctgaaatcc ccgagcgagc accagatatc
     5341 tttgttgagc agtaatatca cgtgataatg tttcctgcct ttttccttgc caaactcccg
     5401 acaccagaaa taacgcaggg tggttgcatg cacacgctga ttggcacatc tttttttccg
     5461 ctggtaagcc tgaattttgg ctttcaggga tgcaaaaaaa cgagaaatca ccgctgaatc
     5521 cggatcggga tccatgatgg tgacaggcat atactcaggg aaacgtaaat ccacccggat
     5581 taacgagacg gcacggtgct gcgccggggc actgtcgaga aggctgtcaa aacgggataa
     5641 ccagtaagca ttgtgtattg attgattcat gatgagagat ctccgttgat ttggattgac
     5701 ttcacatcat tcatcaacgc ggtgaaactt tacggcatcc cgccaatgag caacgccaat
     5761 agctcctgat taaaacggaa tgaatcgtcc cgtaacgaaa agataatcct gcacacgaca
     5821 gcaaaacgta aaacaacagc atgaatgagc taaatgttta aagaaaaaat gtcttttcac
     5881 attatttttc agcaagtaat cataccagca tccctaacat acgaaatcac atagtgatcg
     5941 atcgctgatt cgcttaacaa ctgatcgaat acaccggaat ttacactaac atcctgatgg
     6001 gtaacggaag actaaaaaac ctgtgttcta ttgattgcac ggaatgcctg tgcactggcc
     6061 tgaccagtaa gcacaattct acgagtagta agatatctac gccagaacca tgcggcagta
     6121 gccaccatat gttgccaggg tgaaaaataa gctcagagct ataggggagt aattcccgtt
     6181 aatgtcatcg ctgtacctca atcagttcac aaactggctg aaacgttaag ctgaagcctt
     6241 tattcttcag atgtattttc cggtttacct gtaaattatc ttctgatatt tatcacatta
     6301 gtagttgtgc tgattatcta catacttcaa ggtctgaaat atgaagctaa cagaagaaag
     6361 tctgaatcta gtcattgaca tcctgaatga tttgatctgt gaaggaaaaa tgctgaacga
     6421 tgtggagcgc agatctctgc tcaatgcagc tatgacaatt acagcggtaa aagaacgttc
     6481 tgtcgtatct gtatcatccc gaaaagagca aagtaagaag gagaagaagg aaagagagat
     6541 cgatcctcgt ttcccaaatg ctggtgtggc atggaatgag gcagacgaga gtttattgcg
     6601 agatgttctg gaggatgtac cggacgatga aattggtaaa caccttttct ggctggcaga
     6661 gaaactaggg cgaaccccgt acagtgtagc ctgtaaagtc agacaaatta agaaattacc
     6721 tgcagaatgg aaggatcaat ttcgtaaaat ctctgacagg atccgtagtt ctggaatgtc
     6781 agtcagtgaa tatctacaac gccagggact gttaagccca gagtcagggg aagaaaacaa
     6841 tcctgtcagt cataacaaca atgtagctta atcctgaaga agatgaacag tttatactct
     6901 ttaaactgtt cccttaattt tagaaaaaca gtttgtgtaa gtgcatgctc caacattctg
     6961 aaggagttat ttcttacaat acatcataat cattggcaga tcctcatctg aatcaaaaca
     7021 ccacaaccaa tcatcacact ttttctgaaa agagccttat gatgaagata atcaccttac
     7081 gctgagaaaa aacaccaggg ccagttaatc accttatcaa tctcgccgtt acaaaatcat
     7141 ccataagcca tttaacagtg ttatcgcaaa ctcacagtaa atcgcatcag gttacctcaa
     7201 tcggatttga gttaattcac tgggctttac gtaaagatac ttttctcagg ttaacgacta
     7261 aaacacgaaa actcagaaaa ggttaaacac aggttcagta aaatcgaata tcatcttgat
     7321 tgcttaaaca ggacagaggg ttttatagtg aggttaactg attttagcga tcgtaaaaaa
     7381 aggactaaca aatttactaa actggatgtg tatcagacaa agcaatgtgt atagcaatgc
     7441 ttaatgaaaa tacttgtatg attgtctatg aaagtacgtg tatatagtat cataccgaca
     7501 ttctcaactc ttccaaacat ccatgcagac tgaatgttgg tttcagtttt tcacggccag
     7561 catggggatt tttgttctga aaacggagca gatttactct ggaattattt tccctgtcgg
     7621 gatatttttc acctgaaaac tttctacaac acaacggctg tataacatca ttctggcata
     7681 ttcattcagg tgagtcggac aagcgataat cctgttcttt accgaacgaa caggcagcga
     7741 taaaaaactg attcagacga catttctgtc tgacaaacat tcacatgcag aagaccggga
     7801 catttattct tcccgaaagg aagtgatacc ttttcttatc atggtagctg cattacctga
     7861 tatctttcag gcgaatcccc tccctgtaaa tctgatcgtc agataaaaga gatgaacatc
     7921 aggcagattt ccgtcaataa cgataagaga tgaccatcgt aaacatatct tccagtaacg
     7981 aactggcacc tgcatattac cgtttattct catacatcat tgaaatattg tcagttataa
     8041 aggacacaca tcaggaaatt atcaccatgt gacaacgtca cagatggtgc tgatatataa
     8101 aaattaacaa aatcgcgcca aaaaaccgcg ccgatattgc gcatctgtaa tccatcattt
     8161 cctttttgaa taaccttccg tggttacccg acatcctgat aatgacgccc atcgtacacg
     8221 cttttctttg ctcagacatc gcatactttt atcctgctca ataccgtctt cctgccagtc
     8281 ttcccactct ctgacactca cagaacagca acgtcctccg gacatactag cagggcagaa
     8341 cacaatccac aacattaagt atatgtaaca ataatatacc attaagacca cttctatgac
     8401 cttatatgtt acaaccttag agcatgtatt taatatccca taatacagac tcgtcattgt
     8461 tcttctgaga cgacatgggc atgtcacaaa actaattgac tgacatatca tgaaaatgtc
     8521 agtatttaat aatttaatgg aggtacaaac atgagcgcag atggcatttc cgatttaata
     8581 aaaccagaaa tatttcttgt catttttcct ggtacaacaa ccacagcaaa acggggtggc
     8641 gccgggtggt tgaataataa aagagtcgtg ttccgggtat ggctttatat cagtaaagcc
     8701 agttataaga agatggggat ttttcagcgc ggttctgcgc ccgaatcaat accctatcgt
     8761 gaataccatt ttccctcata ccctgacacc catgaaaaaa ggaaaaaggt attacgtgac
     8821 tggatatcct tcatgcgctc ccgttttaat ataaatcaga tagctcagtt ctgttatcgt
     8881 atggaaaatg aatgggcatt cacctttgat aatatccacg ttccacgaca ctttcgggag
     8941 ggaactgatg acacagaatg gtcatggaat tacattaaga aaaaacgatt attttccctg
     9001 agcggcctgc aacacattca gcctcagaac aatactgaac gaaagcttct cattaatgct
     9061 ttttttgata cctgccttat tgaaaacaac cctgctgacg atattgacat aaagaacatg
     9121 ttactcagaa agatgaatgc agcgttttat aaaagaaaga gccgggcgcg tgcgcgaatt
     9181 cactcccgga ataaccaaca gatgtcagtc agttcaggaa cagataaaca ggtaaatatt
     9241 cgtcttaagg attttcagta tcagcaactt ctttttatcg cgcagaaaga aaacaaaagt
     9301 aagtcagaaa tcataaagga actcattacc agggcatacc gggcgataca ataaccacca
     9361 cgttcaccat ttcatcccga ccatacatca tatgcacggg atgatactga ctatttttta
     9421 tcgtgccgac tgactatgcc aggtatacat aacgaatatt cttttgttac atcgcttaca
     9481 tattcgcgta taagatccct tttcttatta ttatcacgtc cactttctat tctgaagaat
     9541 aattctctta aactaaacct ggacattata tccgaatgca acaatgtaaa aaataacaca
     9601 tgggccagtt cttcacttaa cgacatatca ttttctacag gct
//
LOCUS       DAZGHF010000163         9613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGHF010000163 DAZGHF010000000
VERSION     DAZGHF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.164"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            219..1376
                     /gene="acrE"
                     /locus_tag="ACHK3A_004323"
     CDS             219..1376
                     /gene="acrE"
                     /locus_tag="ACHK3A_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO8742329.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1388..4492
                     /gene="acrF"
                     /locus_tag="ACHK3A_004324"
     CDS             1388..4492
                     /gene="acrF"
                     /locus_tag="ACHK3A_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO8742330.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4745..4966
                     /locus_tag="ACHK3A_004325"
     CDS             4745..4966
                     /locus_tag="ACHK3A_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742331.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5397..6422
                     /locus_tag="ACHK3A_004326"
     CDS             5397..6422
                     /locus_tag="ACHK3A_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8742332.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAFNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6490..7671
                     /gene="yhdX"
                     /locus_tag="ACHK3A_004327"
                     /pseudo
     CDS             6490..7671
                     /gene="yhdX"
                     /locus_tag="ACHK3A_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            7681..8784
                     /gene="yhdY"
                     /locus_tag="ACHK3A_004328"
     CDS             7681..8784
                     /gene="yhdY"
                     /locus_tag="ACHK3A_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO8742333.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKNQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            8792..9550
                     /gene="yhdZ"
                     /locus_tag="ACHK3A_004329"
     CDS             8792..9550
                     /gene="yhdZ"
                     /locus_tag="ACHK3A_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO8742334.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
BASE COUNT         2289 a         2278 c         2485 g         2561 t
ORIGIN      
        1 gaggatttga ttctacctct ttaaacccgc gtcaaaataa aacagtagaa tattaatctt
       61 tttttgtgtt tatgtgcctt gagacgccag tattcataac tattccttac atcgacgaat
      121 gataatttgt aggatagcga actgtatttt tctttctgcg agttaacgcg ttgccttttt
      181 gggtaaataa cgcgcttttg gttttttgag gaatagtaat gacgaaacat gccaggtttt
      241 tcctcctgcc ctcctttatt ctgatctccg cggctttaat cgccggttgt aacgataagg
      301 gagaagagaa agctcacgtc ggtgaaccgc aggttaccgt tcatattgtg aaaacggccc
      361 cgttagaagt taagactgaa ttaccaggcc gcaccaatgc ttaccgtata gccgaagttc
      421 gcccacaggt tagcgggatc gtactgaatc gcaatttcac tgaaggcagc gatgtgcaag
      481 caggccagtc cctgtaccag atcgatcccg cgacctatca ggcaagttat gacagcgcga
      541 aaggcgaact agcgaaaagt gaagccgccg ccgccatcgc gcatttgacg gtaaaacgtt
      601 acgttccgct cgtgggtacg aaatacatca gtcagcagga gtacgaccag gccattgctg
      661 atgctcgcca ggccgatgcc gccgtgattg ccgcaaaagc tacagttgaa agcgctcgca
      721 tcaatctcgc ttataccaaa gtgactgcgc ctattagcgg acgtatcggc aaatcgactg
      781 tgaccgaagg cgctcttgtc actaatgggc aaacgactga actggcgact gtccagcagc
      841 tcgatcctat ctacgttgat gtgacccaat ccagcaacga ttttatgagg ctgaagcaat
      901 ccgtagagca aggaaatttg cataaggaaa acgccaccag caacgtagag ttggtcatgg
      961 agaacggtca aacctatccc ctgaaaggta cgctgcaatt ctccgatgtg accgttgatg
     1021 aaagcaccgg ctccataacc ctacgtgctg tcttccctaa cccgcaacat acgcttttgc
     1081 cgggtatgtt tgtgcgcgca cggattgatg aaggcgtcca acctgacgcc attcttatcc
     1141 cgcaacaagg cgttagccgc acaccacgtg gtgatgcaac cgtgctgatt gttaacaata
     1201 aaagtcaggt tgaagcgcgc cctgtcgttg ccagccaggc gattggcgat aaatggttga
     1261 ttagcgaagg gctgaaatct ggcgatcaag tcattgtcag cggcctacaa aaagcgcgtc
     1321 cgggagagca ggttaaagcc actaccgata cccccgcaga tactgcatcg aagtaaggca
     1381 atctgacatg gcaaactttt ttattcgacg accgatattt gcatgggtgc tggccattat
     1441 tctgatgatg gcgggcgcac tggcgatcct acaattgccc gtcgctcagt atccaaccat
     1501 tgcaccgcct gcggtttctg tttcagcaaa ctatccgggc gctgatgcgc agaccgtgca
     1561 ggatacggtg acgcaggtta tcgaacagaa tatgaacggt atcgataacc tgatgtatat
     1621 gtcctccacc agcgattccg ccggtagcgt gacaattacc cttaccttcc agtccgggac
     1681 cgatcctgat atcgcgcaag tgcaggtgca gaacaaactc cagctcgcca cgccgttgct
     1741 gccgcaggaa gtacagcagc aggggatcag tgttgaaaag tccagtagca gctatttgat
     1801 ggtggcgggc tttgtctctg ataacccagg caccacacag gacgatatct cggactatgt
     1861 ggcctctaac gttaaagata cgcttagccg tctgaatggc gtcggtgacg tacagctttt
     1921 cggcgcacag tatgcgatgc gtatctggct ggacgccgat ctgctaaaca aatataaact
     1981 gacaccggtt gatgtgatta accagttgaa ggtacagaac gatcagatcg ctgccggaca
     2041 gttgggcgga acgccagcgt taccagggca acagttgaac gcctcgatta ttgctcagac
     2101 gcggttaaaa aatccggaag aattcggcaa agtgaccctg cgcgtaaaca gtgacggctc
     2161 ggtggtacgc ctgaaagatg tcgcacgggt tgaacttggc ggtgaaaact acaacgttat
     2221 cgctcgtatc aacggaaaac cggcggcggg cctggggatt aagctggcaa ccggcgcaaa
     2281 tgctctcgat accgcgaaag ccattaaggc aaaactggcg gaattacagc cattcttccc
     2341 gcagggaatg aaggttctct acccttatga caccacacca ttcgtccagc tttctattca
     2401 cgaagtggta aaaacgctgt tcgaagccat tatgctggtg ttcctggtga tgtatctgtt
     2461 cttgcagaat atgcgagcaa cgctgatccc caccattgcg gtacccgtgg tgttgttagg
     2521 gacgtttgcc atcctcgccg cttttggtta ctccatcaac acactaacga tgttcgggat
     2581 ggtgcttgcc atcgggctgc tcgtcgatga tgcgatagtg gtggtggaga acgtcgagcg
     2641 cgtgatgatg gaggataagc tcccgccaaa agaagcgacg gaaaaatcaa tgtcgcaaat
     2701 ccagggcgca ctggtgggta tcgcaatggt gctgtcagcg gtatttattc cgatggcatt
     2761 cttcggcggt tctactgggg caatttatcg tcagttctct atcaccatcg tttcggcaat
     2821 ggcgctttct gttctggtgg cattgattct tacccctgcg ttatgtgcaa cgctgcttaa
     2881 acccgtctct gctgagcatc acgaaaataa gggcggtttc ttcggctggt ttaataccac
     2941 cttcgatcat agcgttaacc actacaccaa cagcgtcggc aaaatcctcg gttccacagg
     3001 acgatattta ctgatctatg cactaattgt tgcaggaatg gtggtgttgt ttttacgtct
     3061 tccgtcttcc ttcttacctg aagaggatca gggtgtcttt ctgactatga ttcagttacc
     3121 cgctggcgcg acgcaagagc ggacgcaaaa agtactggat caagtgacgg attactatct
     3181 gaagaacgag aaagcgaacg ttgaaagtgt ctttacagtt aacggcttta gcttcagcgg
     3241 ccaggcacaa aacgccggta tggctttcgt cagtctgaaa ccgtgggaag agcgtagtgg
     3301 tgacgaaaac agtgcggaag cggtaatcca tcgtgccaaa atggaattgg gcaagatccg
     3361 cgacggtttt gtcattccat tcaatatgcc agccattgtt gaactgggca cggcaacggg
     3421 tttcgacttt gagttaattg atcaggctgg gctgggtcat gatgccctga cccaggcccg
     3481 taaccagttg cttggcatgg cggcgcaaca tcctgccagc ttagtcagcg tgcgtcctaa
     3541 tggtctggaa gacaccgcgc agtttaaact ggaagttgac caagaaaagg cgcaggcatt
     3601 aggtgtttca ctttctgaca tcaatcagac catttcaaca gcgttgggtg ggacttacgt
     3661 taacgacttc attgaccgtg gtcgcgtaaa aaaggtgtat gttcaggcgg atgccaaatt
     3721 tcgtatgctg ccagaagatg tcgataaact ttatgtccgt agcgccaatg gcgaaatggt
     3781 gccattctct gcctttacca ctgcacattg ggtgtatggt tctccgcgac tggagcgcta
     3841 caacggtctg ccgtcaatgg agattcaggg ggaagccgcg ccaggaacca gttccggcga
     3901 tgccatggcg ttgatggaaa accttgcatc acaattacca gctggtattg gttatgactg
     3961 gacgggtatg tcatatcagg aacgcttatc gggaaaccag gctcccgctc tggtagcaat
     4021 ttcctttgtg gttgttttcc tgtgccttgc tgcactctat gaaagctggt caattcctgt
     4081 ctcggttatg ttggtcgtgc cgttagggat tgtcggcgtg ctactggcgg cgacactctt
     4141 taatcaaaaa aatgacgtct actttatggt gggcttgcta acgacaattg gcttgtcggc
     4201 taaaaacgct atattgattg ttgagttcgc taaagatctc atggagaaag agggtaaagg
     4261 tgttgttgaa gcgacactaa tggcagtacg tatgcgtctg cgtcctatcc tgatgacctc
     4321 tctcgccttt attctcggcg tattaccgct agctatcagt aacggtgccg gcagtggcgc
     4381 gcagaacgca gtgggtatcg gggtaatggg cgggatggtt tctgcaacgt tgctggcaat
     4441 cttcttcgtg ccggtgttct ttgtggtgat ccgccgttgc tttaaaggat aaatcagaaa
     4501 cataaaggca ctttcgggtg cctttattat ttccagtgaa acccataaaa attaataaga
     4561 tattcctcta ctcactcttt aaaaactttc tatagttccc gctcccttca ctatttttac
     4621 aattcacata atttgtcatt attcattccg caagatttat aacctccagt gataaaatgg
     4681 cattcagctc gttaataaga gagttaactt attaagcgtt agcgtttatt actgaggtaa
     4741 caccatgaaa agattaattc ctgtggcatt gctcaccgca ttgctggcgg gctgtgcgca
     4801 cgattcgccc tgcgttccgg tttatgacga tcagggtcgt ctggttcaca ccaatacctg
     4861 tatgaaaggc acgactcagg ataactggga aacggctggg gctattgccg gcggggcagc
     4921 agctgttgct ggcctgacga tggggatcat cgctctgtcg aaataactaa acagggcgcg
     4981 gagctccgcg ctctttttaa cgatacagta tctttccata agataaaata ttgctatatt
     5041 ctgctgggta attccctgct gatttagcat gttatatcac gttatttcac tgctttcgaa
     5101 ataacgcatc aatttaactt tcacacatcc caccagaaat atactcacgc taacaatcat
     5161 tattctgctt agtaaaaaac gcgtaaatat tttgcgccaa aatgtggcgc atgtttcatt
     5221 ttcgcaccat tgcggggcgc agtttttatt atttcctgtc tacactcagc ttattgcgac
     5281 gcgaaatcgt gcgcaaaagc tggcactact tttgcttata agaggatggc cacagacagg
     5341 taaaagacgt ttcccaaacg tcctataacg ataattttcg cctcacagga agcattatga
     5401 aaaagatgat gatagccaca ctggctgccg ccagcgtgct gcttgccgtt gcaaatcagg
     5461 cgcatgctgg cgcgacgctt gatgccgttc agaaaaaagg ttttgtgcaa tgcgggatca
     5521 gtgatggatt acctgggttc tcttatgccg atgctgacgg taagttttca ggtattgatg
     5581 tagatgtttg tcgtggtgtt gccgctgctg tattcggtga cgatacgaaa gtgaaatata
     5641 ccccgctcac tgcaaaagaa cgcttcaccg ctttacagtc aggggaggtg gatttgctct
     5701 cccgtaatac gacctggact tcatctcgcg atgccgggat gggaatggca tttactggcg
     5761 tcacttatta cgacggcatt ggcttcctga cgcacgataa agcggggcta aaaagcgcga
     5821 aagaactgga tggcgctacc gtctgtattc aggcgggtac tgataccgaa ctcaacgttg
     5881 ccgattactt taaggcaaac aatatgaagt acacaccagt gactttcgat cgctctgacg
     5941 aatcagcgaa ggcactggaa tctggtcgct gcgatacgct ggcctcggat caatcacaac
     6001 tgtatgccct gcgcatcaaa ttaagcaacc cggctgaatg gattgtctta ccggaagtta
     6061 tctctaaaga accacttggg ccggtagttc gtcgtggcga tgatgaatgg ttctcgattg
     6121 tacgctggac gcttttcgcc atgctgaatg ctgaagagat gggcatcaat tcccagaacg
     6181 tcgatgaaaa agcggctaat ccagcaacac ctgatatggc acatctgctg ggtaaagaag
     6241 gcgattacgg caaggatctg aagctggata ataaatgggc ctttaacatc atcaaacagg
     6301 tgggtaacta ctcggaaatt ttcgagcgta acgtaggttc agaaagcccg ctgaaaatta
     6361 aacgtgggca aaataatctc tggaataacg gcggtattca gtacgcgccg cccgtgcgtt
     6421 aagtcggtgt gaggtaacga gcgtcgctac ggcggcgctc tcttcagcgt tatgctttcc
     6481 gaggtttcta tgtctcatcg ccgctcaacc gttaaaggct cactctcctt tgccaaccct
     6541 acggttcgcg cctggttatt ccagatcttt gccgttgttg ctgttgtcgg aattgttggt
     6601 tggctatttc acaacactgt gacgaatctc agtaatcgtg gcattacttc aggttttgcc
     6661 tttctggatc gcggcgctgg cttcggtatt gtccagcatt tgatcgatta ccagcagggt
     6721 gatacctacg gacgcgtttt cattgttggc ttactcaata cgctactggt ttctgcattg
     6781 tgtattgtat tcgcttctgt tctgggcttc tttatcggtc tggcgcgact ttcggataac
     6841 tggctgctac gaaagctttc cacaatttat attgagatct tccgtaatat tccgccgctg
     6901 ctgcaaatct tcttctgata ctttgccgtg ttgcgcaatt tgcccggacc acgccaggca
     6961 gtgagtgcgt ttgatctggc ctttttgagc aatcgtgggc tttatattcc atctccgcag
     7021 ctgggagacg gatttattgc gtttatcgtg actgttgtta ttgctatagc catttctgtt
     7081 ggactattcc gttttaataa aacgtaccag ataaagaccg gacaattgcg ccgaacctgg
     7141 ccggtcgccg ttatgttgat cataggtttg cctttactgg cgcaatggct ttttggcgca
     7201 gcactacact gggatgttcc agccctacga ggctttaact tccgtggcgg gatggtttta
     7261 attcctgaac tggcagcctt aacgctggcg ctttcggttt atacctctgc atttatcgcc
     7321 gagattatcc gcgctgggat ccaggcagtg ccttatggtc aacatgaagc ggctcggtca
     7381 ctgggattac ccaatccggt tacgctacgc caggtcatta ttccccaggc actgcgagtg
     7441 attattccgc cgttaaccag ccagtatctc aacatcgtca aaaactcctc tcttgccgcc
     7501 gctattggct atccggatat ggtttcgctg tttgctggca ccgtgctgaa ccagacgggg
     7561 caagccatcg agacgatagc catgaccatg tcggtctatc tgattatcag cctgactatc
     7621 tcgctgctga tgaatatcta taaccgccgc atcgcgatcg ttgaacgcta aggagtcatg
     7681 atgacaaaag tattgctgtc tcatcccccg cgcccggcga gccataactc aagccgcgcg
     7741 atggtgtggg tgcgaaaaaa tctgttctcc agctggagca atagcctgct gactattggc
     7801 tgcatatggt tgatgtggga actgattcca ccgttgctga actgggcatt tttgcaggct
     7861 aactgggttg gctcaacgcg taccgactgc acaaaagccg gtgcctgttg ggtcttcatc
     7921 cacgaacgat ttggtcagtt tatgtatggg ctttacccac acgaccaacg ctggcgaatt
     7981 aacctcgcat tactgattgg gcttgtgtcg atcgcaccaa tgttctggaa aatactcccg
     8041 catcgcggtc gctatattgc ggtatgggcg gtgatttacc cactgattgt ctggtggctg
     8101 atgtatggcg ggtttcttgg tcttgagcgg gttgaaaccc ggcaatgggg cgggctgacg
     8161 ctaactttaa ttattgcatc agttgggatt gcgggggcgc tgccgtgggg gatcttactg
     8221 gcgttaggtc gccgctccca tatgccgatt gtgcgtatct tatcggtcat ttttatcgag
     8281 ttctggcgcg gtgtaccgct gataaccgtt ctgtttatgt cttcggtcat gctgccgttg
     8341 tttatggcag aaggcaccag tatcgacaaa ttgatccgcg cgctggttgg cgtgatcctg
     8401 tttcagtcag catatgttgc ggaagtcgtg cgaggcggat tacaggcgct gcctaaaggg
     8461 caatatgaag cggcagagtc gctggcgttg ggttactgga aaaaccaggg gctggttatt
     8521 ctgccacagg cgttgaagct ggtgattcct gggctggtaa ataccatcat cgcactcttc
     8581 aaagatacca gcctggtgat catcatcggg ttgttcgatc ttttcagtag cgttcagcag
     8641 gcaaccgttg atcccgcctg gttgggtatg tcgacggaag ggtatgtttt cgccgcactg
     8701 atctactgga tcttctgttt cagcatgtca cgttatagcc aacatctgga aaaacgtttt
     8761 aacaccgggc gtacaccgca ttgaggacac tatgagccaa attttactgc aacctgctaa
     8821 cgcgatgatt acgctggaaa acgtcaataa atggtatggg caattccatg ttttgaaaaa
     8881 tataaattta accgtgcaac cgggagaacg gatcgttctg tgtggccctt ctggttccgg
     8941 taaatcgaca accattcgtt gtattaatca tctggaagaa caccaacagg gacggatcgt
     9001 ggtagatggc atcgagctta acgaagatat tcgcaatatt gagcgcgtca ggcaggaagt
     9061 gggaatggtc tttcagcatt tcaatctctt ccctcatctg accgttctac agaactgtac
     9121 cctggcaccg atttgggtac gcaagatgcc aaagaaagag gcagaagctc tggcgatgca
     9181 ttaccttgag cgggtgagaa ttgccgaaca tgcgcataag tttcccggac agatttcagg
     9241 tggtcagcag caacgcgttg ccattgcgcg ttcgctgtgt atgaagccga aaattatgtt
     9301 atttgatgag ccaacgtcgg cgctcgatcc tgagatggtg aaagaggtgc tggatacgat
     9361 gattgggctg gcgcagtcgg gtatgacaat gctgtgtgta acacatgaga tggggtttgc
     9421 acgaaccgtc gctgacaggg tgatttttat ggatcgtggg gagatagtgg aacaagctgc
     9481 accggatgag ttttttgcgc atcctaaatc agagcgtacg agggcatttt tatcgcaggt
     9541 gatccattaa ttgaatgtta gttcgaaaag caaaaaggcc atcctttcgg atggcctttc
     9601 gcttgatttg atg
//
LOCUS       DAZGHF010000164         9448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGHF010000164 DAZGHF010000000
VERSION     DAZGHF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.165"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            127..1059
                     /locus_tag="ACHK3A_004330"
     CDS             127..1059
                     /locus_tag="ACHK3A_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8742335.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(1101..2189)
                     /gene="pdeL"
                     /locus_tag="ACHK3A_004331"
     CDS             complement(1101..2189)
                     /gene="pdeL"
                     /locus_tag="ACHK3A_004331"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFO8742336.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(3066..5099)
                     /gene="betT"
                     /locus_tag="ACHK3A_004332"
     CDS             complement(3066..5099)
                     /gene="betT"
                     /locus_tag="ACHK3A_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO8742337.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            5228..5815
                     /gene="betI"
                     /locus_tag="ACHK3A_004333"
     CDS             5228..5815
                     /gene="betI"
                     /locus_tag="ACHK3A_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO8742338.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            5829..7301
                     /gene="betB"
                     /locus_tag="ACHK3A_004334"
     CDS             5829..7301
                     /gene="betB"
                     /locus_tag="ACHK3A_004334"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO8742339.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFIPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            7315..9003
                     /gene="betA"
                     /locus_tag="ACHK3A_004335"
     CDS             7315..9003
                     /gene="betA"
                     /locus_tag="ACHK3A_004335"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO8742340.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKESVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GAECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGQEALPRSTARYFVANGMPVRAKKMSRDVN"
BASE COUNT         2454 a         2476 c         2386 g         2132 t
ORIGIN      
        1 taagcgaagc gacgactaaa cgcgacgggt attaaaagtt attcaaaaat gattatctat
       61 ctttaaccga tacctctaca gtcatagctg gagaaattca tattcgataa tggcgggaat
      121 ggtttaatga actcaatttt taccgaagag aatctgttgg catttactac cgctgcgcgt
      181 tttggcagtt tcagtaaagc cgcagaagag ttgggtttaa ccacttccgc cattagctac
      241 accatcaagc gaatggaaac agggctggat gtggtgctgt tcactcgcag tacccgaagc
      301 attgagttaa cggagtccgg gcgttatttt ttccgcaaag ctaccgacct gctgaatgat
      361 tttcacgcca tcaaacgcag catcgatact atttcgcagg gtatagaagc acgggtgcgc
      421 atttgtatta accaactgct ttatacgccc aaacacaccg cacgcttatt acaggtactg
      481 aaaaagcagt ttccaacctg ccagattacc gtgacgaccg aagtgtataa cggcgtctgg
      541 gatgcgatta ttaataatca ggcgaatatt gctatcggcg cacccgatac gctgctggac
      601 ggcggcggca ttgattacac ggagattggc gcaattcgct gggcttttgc tatcgccccg
      661 gatcacccat tagcctttgt gccggagccg atcgccgaaa gccagctgcg tctttacccc
      721 aatatcatgg tggaggacac cgcgcatacg attaataaaa aagtgggctg gttgctgcat
      781 gggcaggagt cgattctggt gccagatttc aacaccaaat gtcagtgcca gattttaggt
      841 gaaggaattg gttttttgcc ggattacatg gtccgtgagg cgatggcgca atccctgctg
      901 gttacccgac aaatccataa tccgcgccag gattcacgca tgttactcgc aactcagcat
      961 tctgctacgg gtcaggtgac gcagtggatt aaaaaacaat tttcgccaaa tggcatatta
     1021 acggggatct atcaggattt actgcatcgg gagaattaat cgctgccaac ctcccgatgc
     1081 gctgtgaata gcggtttaca tcaaccacct gctttcatta cccattcaga gataaattta
     1141 ttacccggta ctggcggaga gtacaaatag ccctgcaaaa agtgaacgcc tttgccgatc
     1201 attaaatccg cctgctcctg ggtttctacc ccttccgcca cgatactcag accaggctta
     1261 cgcgccagtt cgacaatatt gtccacaata tgaccggata tttcgtccac gctcgccatt
     1321 tgcacaaatg acttatcgat cttaataaaa tcgaccggga acgcctgcaa gtaacgatag
     1381 gtcgcataac ccgtaccaaa gtcatccagc gcaaaggtaa tgttgtgctg atgaaggctg
     1441 tcaaatatcg ctctggcttc tggcgttacc ggaatagggt tacgttccgt tagctcgaga
     1501 accagtttga ttttatcgtt acctaatttc ttaaccaggt tcagacactc tttttcaaat
     1561 cccgcagcca aaaaacaacc cgccgagacg ttgatgccaa tatggaaatt gtccggcagc
     1621 aaatgtttta ccggcatcag aatatccgca gtctgtttca tcaactggcg ggtcattatg
     1681 acaataagac cggatgactc cgccagagga ataaactgat ccggtgggat aattcccgtt
     1741 tgtggatgtt cccagcggac aagcacctca cagcccgtca gtaccccagt ctgcgcgcag
     1801 aaaaccggtt ggatccacgg tttaaattca tggttttcca acgccagact gatggcttca
     1861 ggcgtgacga tatggtgata gtgattatca ttaatatatt tgtgactatt attacccgtg
     1921 gcggatatga tctccggatt gtactgaaag aaaatatccc gccagaaagt aatatcgctc
     1981 tgaatcccca gtttctcaaa gagctgtttc ttttgatgtg aaatggtctt tgcactacga
     2041 tttctgtact gtgatatttc tgttacagac attccctgca tcagcagctt gagtagcagt
     2101 cgttctttct cggatacgct accaggcaat gacaaatgaa ccgttgtacc gaactcttgc
     2161 agaaaaacac gaaaatcaca tgaattcatg aacacacctt ttatctttta tcgctgttca
     2221 gtatgagtga ttacctgtgg attcctgagc acacataagg ggtaaccata aacattagcg
     2281 atgccatacg tataacatcc cacatcacta tcaagaagat tatctttacc tctgatgctc
     2341 gctcagaaac agttacgcta aataccttaa aaaggtaatc acaagtcaaa actcaggaga
     2401 taatcacgca agaatgcaat attaaaattc agatactcat ttttggtaat aaagaagtag
     2461 ttggactatt tcgatattta taatttccca ctacgtattg acgcccattc atgagtaaag
     2521 gcgcattata tgaatttaac aatccagatc aactgaaaat acctcaacct cataaacaca
     2581 tagcgtcaac attcaatgac ataatgagta aagatgttgg ttatgcatac acgtcattac
     2641 tctatgccta tcccttaaca acctgcctct taagactgaa tccattcagc aatagatgac
     2701 caacattata acaatgacaa taaaaacaat atttaaaaca atcaataaaa tatcagacac
     2761 agacaattaa accttaaaca acaaatggtt acattgattt ttgttgattt gcgctacgcg
     2821 ttccccatga ataaagcgtc atttcaaatt agcaccaata tttacattaa ttaagactaa
     2881 gcttattgaa cattcacaac agtccaaacg taggttttgg gcttcaatag ttcttttctt
     2941 ttttattatc tgttgcctgg caatcatcaa tctatactaa gggcttaact gtacagatat
     3001 aacgtatcaa gaggtaagag aaaccgcgcc atatgcatac atggcgcggt tttatgcaat
     3061 aacaatcacg cgtccgggaa catcacgcta tggcccggcg cttcacgatg gagatgaata
     3121 aagttaaggt gccgctcgta ctggtcaaga atatcggtga tcacctgctc tttgctgtag
     3181 tccatcaggt cgttgccctg gctgccttct aacaggaagg tttccagccg gtagtaggtc
     3241 gatttaccgc tacgtgcgcg gtaggtaaag cccggcaccg aatattgctg cggccaaatc
     3301 tgatagacaa agttttgttc ttcgcccata tgcaccaaca aatccagatg ccccaactgt
     3361 tgtccctctt ccggcggcag gctttttagc tccacgtatg cgccgcgcaa ccgcagctcc
     3421 tgcgccactt cttccattgc cgggtaacag accgtctcca tcatctgttt agtgtaacgc
     3481 gtgcccggat aattcatcag gcgcgagaga cgttttttcc agctcaggcg atcctgaagc
     3541 cccagcggtc gcggcgcggt atcgcggttc gcactttcac ggcggtaatc ttctaccttc
     3601 agagatttat acaaccccgc catcacgaag aaaatcacaa agctgaacgg cagccccatg
     3661 attaccgtgg tgttttgcag cgcggatatc ccgttggtca tcagcatgcc gagcgtcagc
     3721 aggccaatcg ccaccgacca gaagacgcgc agccagccgg gggcgtcgct gttgatatct
     3781 ttaagctgcg aggtgaaatt ccccagcacc agcgcaccgg agtccgccga ggtcacataa
     3841 aacagcaggc cagtaatggt ggcgacggag gcgctaaagg taaacgccgg atattgcgcc
     3901 agcaggctgt agaagccgcg ctccggatgg accatcgctt cctcggcaaa tgccgcgccg
     3961 ccgtggatga tttcatacag cgcgctattg ccgaataccg agagccataa cagcgtgaag
     4021 gtaaacggaa taatcaacgt gcccagcacg aactggcgaa tggtacgccc acgcgagata
     4081 cgcgccagga acaagccgac aaacggcgac catgccaccc accatgccca gaagaagagc
     4141 gtccagttat tcatccactc aaccggacgg tcgaaggcaa aactgttgag cgtcatgccc
     4201 ataaagcgat tcacatagtc gccaacattc agcaccagtg cattaagcag gaacgaggtg
     4261 tcgcccataa acaacacgaa caggatcaat cccagcgcca gcgcaacgtt gagttccgat
     4321 aacacgcgaa tgcccttatc aacaccggat gtcaccgaga tagtggcgat gatcaccgac
     4381 aaggcgatca gtgccgcttt cgccgccatc gaatcgggaa tatcaaacag tacgctcaag
     4441 ccatagttaa gctgcaccac accgataccg agcgtagtgg caataccgaa gatggtgccg
     4501 atcaccgctg caatatccac tgaatgacca atcggcccgt taatccgttt accgaagatc
     4561 gggtacagcg ccgagcggat ggtgagcggc aaattataac gataactaaa gtatccgagc
     4621 gccatgccca tcagcgcata catcgaccag ccggttaagc cgtagtgaaa cagcgtccag
     4681 accatcgcct gacgcgcggc ctcaatcgtc tgtcccgcgc cttccggcgg ctgcatatac
     4741 tgcgttaccg gttcggctac ggagaagaac atcaggtcga taccgatccc ggcagcaaac
     4801 agcatcgccg cccaactcag cagactgaat tccggtttgg attgttctgg cccgagcttc
     4861 accgaaccaa aacgcgaaca agcgatacag accacaaaga caatatagag cgttgccgcc
     4921 agcagatagt accaaccgaa ggttttagaa acccagtcca gcgtgcggcc aatccacagg
     4981 gccgagaagt cgcgaaacag gatcgttgtc agggaaaaca acaaaatcag tccggcggag
     5041 gtgtaaaaca ccaccggatt gattttgtcc ttttccctgc tgtgtgaaag gtctgtcatc
     5101 cagtatcccc actgttattg ttaccattta aaatcaaatt cgtaacaatt aagacacatt
     5161 ttatattgaa cgtccaatca ataaccgctt taatagataa acaacgctga tgaatggagt
     5221 ggcgaaaatg cccaaattgg ggatgcagtc gatccggcgc agacaactga tcgacgccac
     5281 gctggaagca ataaatgaag tgggcatgca cgatgcaacg atcgcgcaga tcgcccgccg
     5341 tgcaggcgtt tctacgggga tcatcagcca ctatttcagg gacaaaaatg gtctgctgga
     5401 agcaaccatg cgcgatatca ccagtcagct gcgtgacgcg gttttgaatc gattacatgc
     5461 acttccgcag ggcagtgcag agctgcgatt acaggcgatt gttggtggaa acttcgatga
     5521 aacccaggtg agcagtgcgg cgatgaaagc ctggctggcc ttctgggcca gcagtatgca
     5581 tcagccgatg ctctatcgct tgcagcaggt cagtagccgc cgcttattgt cgaatctggt
     5641 gagcgaattt cgccgcgagt taccgcgcca acaggcacag gaagcgggct acggccttgc
     5701 cgcgctgatt gacggattat ggctgcgcgc ggcactgagc ggcaaagcgc tggataaacc
     5761 tctcgcacac tcgctgaccc gtcactttat tactcagcat ttacccaccg attaaccgag
     5821 gaaacgtgat gtcccgaatg gcagaacagc agctttatat acatggtggt tatacctccg
     5881 ccaccagcgg tcgcaccttc gagaccatta acccggccaa cggtaacgtg ctggcgaccg
     5941 tgcaggccgc cgggcgtgag gatgtcgatc gcgccgtgaa gagtgcccaa caagggcaaa
     6001 aaatctgggc ggcgatgacc gctatggagc gctcgcgtat tctgcgtcgg gccgttgata
     6061 ttctgcgtga acgcaatgac gaactcgcaa aactggaaac cctcgacacc ggaaaagcgt
     6121 attcggaaac ctcaaccgtc gatatcgtta ccggtgcgga cgtgctggag tactacgccg
     6181 ggctgatccc gtcgctggaa ggcagccaga tcccattgcg tgagacgtca tttgtttata
     6241 cccgccgcga accgctgggc gtagtggcag ggattggcgc atggaactac ccgatccaga
     6301 ttgccttgtg gaaatccgcc ccggcgctgg cggcaggcaa cgcgatgatt ttcaaaccga
     6361 gcgaagtcac cccgcttacc gcgttaaagc tggccgaaat ttacagcgaa gcgggcctgc
     6421 cggacggcgt atttaacgtg ctgccgggcg taggtgcgga aaccgggcag tatctgaccg
     6481 agcatccggg cattgccaaa gtgtcattta ccggcggtgt cgccagcggc aaaaaagtga
     6541 tggctaactc ggcggcttcc tccctgaaag aagtgaccat ggagctgggc ggtaaatcac
     6601 cgctgatcgt tttcgacgat gcggatctcg atctcgccgc tgatatcgcc atgatggcga
     6661 acttcttcag ctccggtcag gtgtgtacca acggcacccg cgtgtttatt ccggcgaaat
     6721 gcaaagccgc atttgaacaa aagattctgg cgcgcgttga gcgcattcgc gcgggcgacg
     6781 ttttcgatcc gcaaactaac tttggcccgc tggtcagctt cccgcatcgc gataacgtgc
     6841 tgcgttacat cgccaaaggc aaagaggaag gcgcgcgcgt actgtgcggc ggcgatgtac
     6901 tgaaaggcga tggcttcgat aacggcgcat gggttgcacc gaccgtgttc accgattgcc
     6961 gcgacgaaat gaccatcgtg cgcgaagaga tcttcggtcc ggtgatgtcg cttctcactt
     7021 atgaatctga ggacgaagtg attcgccgcg ccaatgatac cgactacggc ctggcggcgg
     7081 gcatcgtgac ggcggacctg aaccgcgcgc atcgcgtcat tcatcagctg gaagcgggta
     7141 tttgctggat caacacctgg ggcgaatccc ctgcagagat gcccgttggc ggctacaaac
     7201 actccggcat tggtcgcgag aacggcgtga tgacgctcca gagttacacc caggtgaagt
     7261 ccatccaggt tgagatggct aaattccagt ccatattcta accaggaggt ttatttgcaa
     7321 tttgactaca tcattattgg tgccggctca gctggcaacg ttctcgctac ccgtctgact
     7381 gaagatccga acacaaccgt gctgctgctt gaagcaggcg gcccggacta tcgctttgac
     7441 ttccgcaccc agatgcccgc cgcgctggcg ttcccgttac agggtaaacg ctataactgg
     7501 gcctatgaga cggaaccgga accgtttatg aacaaccgtc gcatggagtg cggacgcggt
     7561 aaggggcttg gcgggtcatc actgatcaac ggcatgtgtt acatccgtgg taacgcgctg
     7621 gatctcgata actgggcgaa agaacccggt ctggagaact ggagctacct cgactgtctg
     7681 ccctactatc gcaaggccga gactcgcgat gtgggcgaga acgactacca cggcggtgat
     7741 ggcccggtga gcgtcaccac ctccaaaccc ggcgtcaatc cgctgtttga agcgatgatt
     7801 gaagcgggcg tgcaggcggg ctacccgcgc acggacgatc tcaacggcta tcagcaggaa
     7861 ggttttggcc cgatggatcg caccgtcacg ccgcagggcc gtcgcgccag caccgcgcgt
     7921 ggctatctcg atcaggccaa atcgcgccct aacctaacca ttcgtactca cgccatgacc
     7981 gatcacatca tttttgacgg caaacgcgcg gtgggcgtcg agtggctgga aggcgacagc
     8041 accattccga cccgcgcggc ggcgaataaa gaagtgctgt tatgtgcagg cgcgattgct
     8101 tcaccgcaga tcctgcaacg ctccggcgtc ggcaacgctg aactgctggc ggagtttgat
     8161 attccgctgg tgcatgattt acccggcgtc ggcgaaaatc ttcaggatca cctggagatg
     8221 tatctgcaat atgagtgcaa agaatcggtt tccctctacc ctgccctgca gtggtggaat
     8281 cagccgaaaa tcggtgcgga gtggctgttt ggcggcaccg gcgttggtgc cagcaaccac
     8341 tttgaagcag gcggatttat tcgcagccgt gaggaatttg cgtggccgaa tattcagtac
     8401 catttcctgc cagtggcgat taactataac ggctcgaacg cagtgaaaga gcacggcttc
     8461 cagtgccacg tcggatcgat gcgctcgcca agccgtgggc atgtgcggat taaatcccgc
     8521 gacccgcacc agcatccagc gattctgttt aactacatgt cgcacgagca ggactggcag
     8581 gagttccgcg acgcaattcg catcacccgg gagatcatgc atcaaccggc gctggatcag
     8641 tatcgtggcc gcgaaatcag ccccggcgcg gaatgtcaga cggatgagca gctcgatgag
     8701 ttcgtgcgta accacgccga aaccgcgttt catccgtgcg gtacctgcaa aatgggttat
     8761 gacgagatgg cagtggttga cgccgaaggc cgcgtacacg ggctggaagg cctgcgcgtg
     8821 gtggatgcgt caattatgcc gcaaattatc accggcaatt tgaacgctac gacgattatg
     8881 attggcgaga aaatggcgga tatgattcgc gggcaagaag cgttgccgag gagcacggca
     8941 agatattttg tggcaaatgg gatgccggtg agagcgaaaa aaatgagtcg tgatgtgaac
     9001 taactcagga acctatcaga ttgagagtta ccgttctact atgaggaccg aatccttata
     9061 taaaacactg aggtaactct catgcttcat atccgcagtc ccgccatcaa actttaagcc
     9121 gattcgctca atctggcgga tgatggtgct atggatacgc tgataaacca caagtgtaat
     9181 gccactcagc ttaagaatca cgcaaccagt gctcctggct agggttactt gttctttccc
     9241 actcccggtt agcagtggtc agcgatcaac tgcgtaagcc gggcgtattt atcccaaagc
     9301 gtggagtaat atcaggtcta cgctaaaaaa gtcaatttat tcggttatca tgcacgaaaa
     9361 ggggacttgt ggcgagtata aaaataagtc agccccaaaa actacgtgga gctttaaaca
     9421 cggttcgtat aatttattag cagcgatt
//
LOCUS       DAZGHF010000165         9318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGHF010000165 DAZGHF010000000
VERSION     DAZGHF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.166"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            346..1686
                     /gene="dcuB"
                     /locus_tag="ACHK3A_004336"
     CDS             346..1686
                     /gene="dcuB"
                     /locus_tag="ACHK3A_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO8742341.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1764..3410
                     /gene="fumB"
                     /locus_tag="ACHK3A_004337"
     CDS             1764..3410
                     /gene="fumB"
                     /locus_tag="ACHK3A_004337"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO8742342.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3533..4183
                     /gene="yjdF"
                     /locus_tag="ACHK3A_004338"
     CDS             3533..4183
                     /gene="yjdF"
                     /locus_tag="ACHK3A_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO8742343.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(4180..5535)
                     /gene="melA"
                     /locus_tag="ACHK3A_004339"
     CDS             complement(4180..5535)
                     /gene="melA"
                     /locus_tag="ACHK3A_004339"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO8742344.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5818..6726
                     /gene="melR"
                     /locus_tag="ACHK3A_004340"
     CDS             5818..6726
                     /gene="melR"
                     /locus_tag="ACHK3A_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO8742345.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            6925..9192
                     /gene="adiA"
                     /locus_tag="ACHK3A_004341"
     CDS             6925..9192
                     /gene="adiA"
                     /locus_tag="ACHK3A_004341"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO8742346.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         2298 a         2498 c         2327 g         2195 t
ORIGIN      
        1 aaatgggtag atcagattaa tctataaacc taatgatatc tgccctgaga acaaaaaata
       61 gaccgataaa tatcaataag ataacagcaa acaaaacatt aacatctgcg cagtacaaac
      121 tataaaccca tcgccagaga gtttttctct ctgaaaaagc cgcttatcac agtgcataaa
      181 tttgccgctg ctttaatcag ccaatattca ctgtgaggta tttgctaaag ccggtaacga
      241 ccaaacggat atttagtcag gctctgaaaa cagttcatac aaaacagaac gtgactgtga
      301 tctattcagc aaaaatttaa ataggattat cgcgagggtt cacacatgtt atttactatc
      361 caacttatca taatactgat atgtctgttt tatggtgcca gaaagggtgg tatcgcgctg
      421 ggtttattag gcggtatcgg tctggtcatt ctggtcttcg tcttccacct tcagccaggt
      481 aaaccaccgg ttgatgtcat gctggttatc attgcggtgg tggcggcatc ggcgaccttg
      541 caagcttcgg gcggtcttga tgtcatgctg caaattgccg agaagctgct gcgccgcaac
      601 ccgaaatatg tctcaattgt cgcgccgttt gtgacctgta cgctgaccat tctttgcggt
      661 acgggtcatg tggtttacac cattctgccg atcatctacg acgttgctat taagaacaac
      721 atccgtccgg aacgtccaat ggcggcaagt tctatcggtg cacagatggg gattatcgcc
      781 agtccggtgt cggttgcagt cgtgtctctg gttgcgatgc tgggtaatgt cacctttgat
      841 ggtcgccatc ttgagttcct cgacctgctg gcaatcacca ttccatcgac gttaatcggt
      901 atcctggcga tcggtatctt cagctggttc cgcggtaaag atctggataa agacgaagag
      961 ttccagaaat tcatctccgt accggaaaac cgtgagtatg tttacggtga taccgcgacg
     1021 ctgctggata aaaaactgcc gaaaagcaac tggctggcaa tgtggatctt cctcggggca
     1081 atcgctgtag tcgcccttct tggtgctgat tcggacctgc gtccatcctt cggcggcaaa
     1141 ccgctgtcga tggtactggt tattcagatg tttatgctgc tgaccggggc gctgattatt
     1201 atcctgacca aaaccaatcc cgcgtctatc tcaaaaaacg aagtcttccg ttccggtatg
     1261 atcgccatcg tggcggtgta cggtatcgca tggatggcag aaaccatgtt cggtgcgcat
     1321 atgtctgaaa ttcagggcgt actgggtgaa atggtgaaag agtatccgtg ggcctatgcc
     1381 attgttctgc tgctggtttc caagtttgta aactctcagg ctgcggcgct ggcggcgatt
     1441 gttccggtcg cgctggcgat cggcgttgat ccggcataca tcgtggcttc agcaccggct
     1501 tgctacggtt attacatcct gccgacttat ccgagcgatc tggcagcgat tcagtttgac
     1561 cgttccggca ccacccacat cggtcgcttc gtcatcaacc acagctttat tctgccgggg
     1621 ttgattggtg tgagcgtatc gtgcgtcttc ggctggatct tcgccgcgat gtacgggttc
     1681 ttataaatgc actttgcgtg ccgcccgtga ctacgcggca cgccattttc gaataacaaa
     1741 tacagagtta caggctggaa gctatgtcaa acaaaccctt tatctaccag gcacctttcc
     1801 cgatggggaa agacaatacc gaatactatc tactcacttc cgattacgtt agcgttgccg
     1861 acttcgacgg cgaaaccatc ctgaaagtgg aaccagaagc cctgaccctg ctggcgcagc
     1921 aagcctttca cgacgcttct ttcatgctcc gcccagcaca ccagaaacag gttgcggcta
     1981 ttcttcacga tccagaagcc agcgaaaacg acaagtacgt ggcgctgcaa ttcttaagaa
     2041 actccgaaat cgccgccaaa ggcgtgctgc cgacctgcca ggataccggc accgcgatca
     2101 tcgtcggtaa aaaaggtcag cgcgtgtgga ccggtggcgg tgatgaagaa gcgctgtcga
     2161 aaggcgtcta taacacctat atcgaagata acctgcgcta ttcacagaac gcgccgctgg
     2221 acatgtataa agaggtcaac accggcacga acctgcctgc gcaaatcgac ctgtacgcgg
     2281 tagatggcga tgagtacaaa ttcctctgtg tcgctaaagg cggcggctct gccaacaaaa
     2341 cgtatctcta ccaggaaacc aaagccctgc tgacgcccgg caaactgaaa aacttcctcg
     2401 tcgagaaaat gcgtaccctc ggtacggcag cttgcccgcc gtaccatatc gcgtttgtga
     2461 ttggcggtac gtctgcggaa accaacctga aaaccgtcaa gttagcaagc gctcactatt
     2521 acgatgaact gccgacggaa gggaacgaac atggtcaggc gttccgcgat gtccagctgg
     2581 aacaggaact gctggaagag gcccagaaac tcggtcttgg cgcgcagttt ggcggtaaat
     2641 acttcgcgca cgacattcgc gttatccgtc tgccacgtca cggcgcatcc tgcccggtag
     2701 gcatgggcgt ctcctgctcc gcagaccgta acattaaagc gaaaatcaac cgcgaaggta
     2761 tctggatcga aaaactggaa cacaacccag gccagtacat tccacaagaa ctgcgccagg
     2821 ccggtgaagg cgaagcggtg aaagttgacc ttaaccgccc gatgaaagag atcctcgccc
     2881 agctttcgca atacccggta tccacccgtt tgtcgctcac cggcaccatt attgtgggtc
     2941 gagatattgc acacgccaag ctgaaagagc tgattgacgc cggtaaagaa ctgccgcagt
     3001 acatcaaaga tcacccgatc tactacgcgg gtccggcgaa aacccctgcc ggttatccat
     3061 caggttcact tggcccaacc accgcaggtc gtatggactc ctacgtggat ctgctgcaat
     3121 cccacggcgg cagcatgatc atgctggcga aaggtaaccg cagtcagcag gttaccgacg
     3181 cgtgtcataa acacggcggc ttctacctcg gtagtatcgg cggtccggcg gcggtactgg
     3241 cgcagcagag catcaagcat ctggaatgcg tcgcttatcc ggagctgggt atggaagcta
     3301 tctggaaaat cgaagtagaa gatttcccgg cgtttatcct ggtcgatgac aaaggcaacg
     3361 acttcttcca gcaaatcgtc aacaaacagt gcgcgaactg cactaagtaa cctcttcggc
     3421 ccagcgcctg gcagcatgct gccaggtgat ccccctgggc cacctctttt gcgattgtaa
     3481 tttcacgctt actggtcaat agtcagtatt ttccctgact tgcgaactca ccatgacccg
     3541 cacactcaag ccgttaattc ttaacaccgg cgcactggcg ctaacgttaa tcctgattta
     3601 taccggcatt tcggcccatg acaaactcac ctggctgatg gaagtgacac cggtgattat
     3661 tgtcgtgccg ctactgcttg ccaccgctaa acgttatccg ttaactccgc tgctttatac
     3721 gctcattttc tttcacgcca tcatcctgat ggtcggcggg caatacacct acgcgaaagt
     3781 ccccgttggt tttgaggtgc aggaatggct ggggttgagc cgtaatccgt atgacaaact
     3841 ggggcatttt ttccaggggc tggtgcctgc actggtggca cgagagatcc ttgtacgcgg
     3901 gatgtacgtt cgcggacgta agatggtggc gtttctggtc tgctgcgtgg cgctggcgat
     3961 aagcgcgatg tatgaattaa tcgagtggtg ggcggcgctg gcaatgggtc agggagcgga
     4021 tgattttctc ggcacccagg gcgaccagtg ggacacgcaa tccgatatgt tctgcgcgct
     4081 gcttggcgca ttaacgacgg tgatattcct cgctcgcttt cattgccgcc agctacggcg
     4141 ctttggctta atcaccagag cgccggaagc gattacccct taacggtgca accagcctgg
     4201 cagccagtcg ccgtgggcgg caatcaggtc gtcaacaaga gcatatattt catcaatgcc
     4261 cagcacggcg gcagtatgcg ggtccatcat cgcagcgtgg taaacacggt cgcgattttc
     4321 cgtaagaatg gcttcggtca gcagcgtctg tacgttgatg ttggtttgca tcagggcggc
     4381 cagatgcgaa ggtaacgtac cgactttggt cggctgaatg ccattagcat caaccagaca
     4441 ggctacttcc acgcaacatc cttgtggcag gttatcaatc aatccatcgt tacggacgtt
     4501 gccgtaaatc acactcggct cgccagtcca gatagcgttc atgattgtgc tggcatattc
     4561 ccgtgacggt ttaatatcaa tccgggaggc gtttttatac tcctccagct ctttatgcca
     4621 gttcgccagc tgctcgacgc agcgtttcgg gtactcatcc agcggtactt tataacgctc
     4681 aatcaaatcc tcacgacctg gcttaataaa ccacggtgtg tactcagcaa aatgttctga
     4741 cgattccgtg acgaagtagc ccagcttttt gaacatttcg tagcgcacaa tattctggca
     4801 gcgagtattg ccatgaatat tcggcttcgg tgcttgccct gcttcataag ccgccagcag
     4861 ttccgggtag agattcacgt aactgccgtc ggcggttttg cgctccagtt ccaggtaaaa
     4921 cgccatatgg ttgatacctg cgcaacggta acgcagcgta gccgggtcga tattgaggtc
     4981 acgcgccagc tcttccgccg ttccctgcac cgaatggcac agccctacct gtttgatatg
     5041 cggatagcgg gcatacatcg cccaggtatt catcgccatt gggttaacgt agttgagcat
     5101 ggtggcatcg gggcagactt ccgtcatgtc ctcgcaaatt tgccacagat gcggaatggt
     5161 acgtagcgcg cgcataatac cgcccggccc caacgtatcg gcaatggttt gttccagacc
     5221 atgccgctta cagacctcga aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa
     5281 tgccaccacg acaaaatcgg catcctgtaa ggcttctttc tgttgggtgt ggcaggtgat
     5341 tttgccgctg gcccctgctg aatccatcag cttgcgcacc acaatatgcg actcttccag
     5401 gcgggtggga tcaatgtcca tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa
     5461 cacatcacca agaatatttt taacgaaaat cgtcgaacca gcgccgataa atgtaatttt
     5521 gggtgcagac atcatgcaga tctcctggct tgcttgaata acttcatcat ggcaggcgaa
     5581 tctgcagaaa acctccgtct tccaggttga gcattcccat aaactcagat cctgcgtgaa
     5641 gcagcagtaa atctgagttt atgggaataa gttacctgaa aagcagagtt ttcggcctca
     5701 tgatgctcgg acgctcccct cgccgctgtg ggaagagggt gaaaaccgtg ctcccactcg
     5761 cagtcatcct ccctcactcc tgccataatt ctgatattcc aggaaagaga gccatccatg
     5821 aatacagata cgtttatgtg cagcagcgac gaaaaacaga cccgcagccc gctgtcgcta
     5881 tattcggaat accagcggat ggagattgag tttcgcgcac cgcatatcat gcccaccagc
     5941 cactggcatg gtcaggtcga agtgaatgtg cctttcgatg gcgatgtgga atacctgatc
     6001 aacaatgaaa aagtgaatat caatcagggt catatcacgc tgttctgggc ctgtacaccg
     6061 caccaactaa cagataccgg aacctgtcag agcatggcga tttttaatct gccgatgcat
     6121 ctgtttctct cctggccgct ggataaagac ctgattaacc acgttactca cggcatggtg
     6181 atcaaatcac tggcgacaca gcaacttagc ccgtttgaag tgcgccgctg gcagcaggaa
     6241 ttgaacagtc cgaacgagca aattcgccag ctcgccattg atgaaattgg cctgatgctc
     6301 aagcgattta gcctctctgg ctgggaaccg attctggtca ataaaacctc gcgcacacac
     6361 aaaaacagcg tctcgcgcca tgcgcaattt tatgtcagcc agatgctggg ctttattgcc
     6421 gaaaactatg atcaggcact gaccatcaac gatgtggctg agcacgtcaa acttaacgcc
     6481 aactatgcaa tggggatatt ccagcgggtc atgcaattga cgatgaaaca gtacattacc
     6541 gcgatgcgca tcaaccacgt tcgcgcgtta ctgagcgata ccgataaaag tattctcgat
     6601 attgccctga cggcaggctt tcgttcgagt agccgttttt acagcacgtt cggcaaatat
     6661 gtcggcatgt caccgcaaca ataccgcaaa cttagccaac agcgccgcca gacgtttccc
     6721 ggctaaagac gatatcagta tcagccaaaa aaatagttta gccgtcgata ataagcagtg
     6781 aaaataattt aaaataatca cataacatat tgtttataaa aacaaatatt tcaaaaatgt
     6841 ttgtttttca cgcgctttac agcccgaaaa ggccggaaga tacttgcccg caacgaagat
     6901 tccttcataa ccgggtaagc aatgatgaaa gtattaattg ttgaaagcga gtttctccat
     6961 caagacacct gggtcggtaa cgccgttgag cgtctggcag atgctttaag ccagcaaaat
     7021 gttaccgtga ttaaatccac ctcctttgat gatggttttg ccattctctc ttcaaacgaa
     7081 gccattgact gcctgatgtt cagctatcaa atggaacatc cggacgaaca tcaaaacgtc
     7141 agacaattga tcggtaagct tcatgagcgt caacaaaacg tgccggtctt cctgttgggc
     7201 gatcgggaaa aagccctcgc cgcaatggac cgggatctgc tggagcttgt cgatgaattc
     7261 gcctggattc tggaagatac cgccgacttt atcgccggac gcgccgttgc cgcgatgacc
     7321 cgctaccgcc agcaactgtt gccgccactg ttcagcgcgc tgatgaaata tagcgacatc
     7381 catgaatatt cctgggcagc gccaggccac cagggcggcg ttggttttac caaaacaccc
     7441 gccggacgtt tctaccatga ctactatggt gaaaatctgt tccgcaccga catgggcatc
     7501 gaacgaactt ccctcggttc tttgcttgac catactggcg catttggcga aagcgaaaaa
     7561 tatgctgcac gcgtatttgg tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc
     7621 tctaaccgca ccatcatgca ggcttgcatg accgataacg atgtcgtggt cgttgaccgt
     7681 aactgccata aatccatcga acaaggcttg atgctgacag gcgcgaaacc ggtctacatg
     7741 gtgccaagcc gcaaccgcta cggcattatc gggccaatct atccgcagga aatgcaacct
     7801 gaaaccttgc agaagaaaat cagtgaaagc ccgctgacca aagacaaagc cgggcaaaaa
     7861 ccgtcttact gcgtggtgac caactgtacc tatgacggcg tgtgttataa cgctaaagaa
     7921 gcgcaggatc tgctggaaaa aacctccgat cgtctgcact ttgacgaagc ctggtacggc
     7981 tatgcacgtt tcaacccaat ctatgccgat cactatgcca tgcgcggcga acctggcgat
     8041 cacaacggcc ctaccgtttt cgccacccac tccacccaca aactgctgaa tgcgctgtca
     8101 caggcctctt atattcatgt acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac
     8161 caggcctaca tgatgcatgc caccacctcc ccgctgtatg ccatctgcgc atccaacgac
     8221 gtggcggtgt cgatgatgga cggcaacagc ggcctgtcac tgacgcagga agtgatcgac
     8281 gaagcggttg atttccgtca ggcgatggcg cggctgtata aagagttcac cgctgacggt
     8341 agctggttct tcaaaccgtg gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc
     8401 tatgactttg ctgacgcacc aaccaaactg ctgaccaccg ttcaggactg ctgggtcatg
     8461 catccgggcg aaagctggca cggcttcaaa gatattccgg ataactggag tatgctcgac
     8521 ccgattaaag tcagcatcct tgctccggga atgggtgaag atggtgaact ggaagaaacc
     8581 ggtgttccgg cggcgctggt cacagcctgg cttggtcgcc acggcattgt gcctacccgt
     8641 accactgact tccagattat gttcctgttc tctatgggcg tgacccgtgg gaaatggggg
     8701 actctggtta acaccctttg ctccttcaaa cgccactatg acgccaacac cccgctggcg
     8761 caggtgatgc cggaacttgt tgaacaatat cctgacactt acgcgaacat ggggattcac
     8821 gatctgggtg acaccatgtt tgcctggctg aaagaaaaca accctggcgc acggttgaac
     8881 gaagcctatt ccggcctgcc ggtggcggaa atcaccccgc gagaagcgta caacgcgatt
     8941 gtcgacaaca atgtcgaact ggtatccatt gaaaacctac caggacgcat cgcggcaaac
     9001 tcagttatcc cgtatccgcc aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat
     9061 aaaaacagtc cgcaagtaag ttatttacgc tcgctgcaat cctgggacca tcatttccct
     9121 ggatttgaac acgaaactga agggactgaa attattgacg gtatttacca cgttatgtgt
     9181 gtgaaagcgt aaccactatt ccgctgaagg cgtaattgtt aaataacatt acgccgcctg
     9241 gcttgaggcc ttttaagtat ggcaacgttt tcataaaaat tgctgcaaac aaaaaatgtc
     9301 atgctttttg cgcggtcc
//
LOCUS       DAZGHF010000166         9224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGHF010000166 DAZGHF010000000
VERSION     DAZGHF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.167"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1726
                     /gene="adeD"
                     /locus_tag="ACHK3A_004342"
     CDS             <1..1726
                     /gene="adeD"
                     /locus_tag="ACHK3A_004342"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO8742347.1"
                     /translation="AEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRY
                     IAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVI
                     CDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAW
                     RDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNTYIAAGIE
                     NCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWE
                     IAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSD
                     ARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTISRQPVSASDFALQFTPGK
                     RYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNE
                     GALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVIRNGQVQSHLPLPIAGLM
                     STDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFA
                     FTTLEVTE"
     gene            complement(1773..3164)
                     /gene="uhpT"
                     /locus_tag="ACHK3A_004343"
     CDS             complement(1773..3164)
                     /gene="uhpT"
                     /locus_tag="ACHK3A_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO8742348.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQSFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3420..4739)
                     /gene="uhpC"
                     /locus_tag="ACHK3A_004344"
     CDS             complement(3420..4739)
                     /gene="uhpC"
                     /locus_tag="ACHK3A_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO8742349.1"
                     /translation="MLPFLKAPADAPLLTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGVSALLLLPFLNAQTPREA"
     gene            complement(4749..6251)
                     /gene="uhpB"
                     /locus_tag="ACHK3A_004345"
     CDS             complement(4749..6251)
                     /gene="uhpB"
                     /locus_tag="ACHK3A_004345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO8742350.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6251..6841)
                     /gene="uhpA"
                     /locus_tag="ACHK3A_004346"
     CDS             complement(6251..6841)
                     /gene="uhpA"
                     /locus_tag="ACHK3A_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO8742351.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6914..7204)
                     /gene="ilvN"
                     /locus_tag="ACHK3A_004347"
     CDS             complement(6914..7204)
                     /gene="ilvN"
                     /locus_tag="ACHK3A_004347"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO8742352.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7208..8896)
                     /gene="ilvB"
                     /locus_tag="ACHK3A_004348"
     CDS             complement(7208..8896)
                     /gene="ilvB"
                     /locus_tag="ACHK3A_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO8742353.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAIAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLTHYGLINAVAACVDDSAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9002..9100)
                     /gene="ivbL"
                     /locus_tag="ACHK3A_004349"
     CDS             complement(9002..9100)
                     /gene="ivbL"
                     /locus_tag="ACHK3A_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO8742354.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
BASE COUNT         2227 a         2648 c         2422 g         1927 t
ORIGIN      
        1 ggctgaatac caggaattgt tagccgtttc ccgtggagac gctgttgccg attatattat
       61 tgataatgtc tctattctcg acctgatcaa tggcggagaa atttccggcc caattgtgat
      121 taaaggacgt tacattgccg gtgttggcgc agaatacgct gatgctccgg ctttgcagcg
      181 gattgatgcc cgcggcgcaa cggcggtgcc agggtttatt gatgcgcacc tgcatattga
      241 atccagcatg atgacgccgg tcacctttga aaccgctacc ctgccgcgcg gcctgacgac
      301 cgttatttgc gaccctcatg aaatcgtcaa cgtgatgggc gaagccggtt tcgcctggtt
      361 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtacc
      421 cgccctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
      481 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactatcctg gcgtaattag
      541 cgggcagaat gcgctgctcg ataaactgga cgcatttcgc cacctgacgc tggacggtca
      601 ctgcccgggt ttgggtggta aagaacttaa cacctatatt gctgcgggta ttgaaaactg
      661 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggta tgtcgttgat
      721 gatccgcgaa gggtccgcag cccgcaatct caacgcgctg gcaccgttga tcaacgaatt
      781 taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga
      841 aggacatatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
      901 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
      961 actggcaccc ggtaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     1021 gcaacaggta ctggtgaaag gcgagccgat tgatgcgcaa accttacagg cggaagagtc
     1081 ggcgagactg gcacaatccg ccccgccata tggcaatacc atttcccgcc agccagtttc
     1141 cgccagcgac tttgccctgc aatttacccc cggaaaacgc tatcgcgtta ttgaggtcat
     1201 ccataacgaa ttgattaccc actcccgctc cagcgtctac agcgaaaatg gttttgatcg
     1261 cgatgatgtg tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcctg
     1321 tggtttgctc ggcggctttg gcctgaatga aggtgcgctg gcggcgacgg tcagccatga
     1381 cagccataat attgtggtga tcggtcgtag cgcagaagag atggcgctgg cggtcaatca
     1441 ggtgattcag gatggcggcg ggctgtgcgt gatccgtaac ggtcaggtac aaagtcatct
     1501 accgttgccc attgccgggc taatgagcac cgacacggcg cagtcactgg cggagcaaat
     1561 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta ccggatgagc cgtttattca
     1621 gatggcgttt ctttccctgc cagtgatccc cgcgctgaaa ctaaccagtc aggggctgtt
     1681 tgatggcgag aagtttgcct tcactacact ggaagtcacg gaataataaa aaaaagcccg
     1741 gcgtcatgcc gggcaaaagt caccagttaa gtttatgcca ctgtcaactg ctgaattttt
     1801 ttctcgcggc ggattttgcg ttcttccatc accgccacta tcgccatcag gcagatacaa
     1861 ccaatcgcgg cgatatccag cgcggcgaag gtgcctgccc agccggtaag gccaaatacc
     1921 ggcgtcccat cggcaatcat tcccagacct aacttggcaa agctgtcacc aatcaggtaa
     1981 gcgaaggtgc ctttaatacc atcggcagcg ccaatcgctt ttttaggtac aaagccaaca
     2041 gcagccacac caatcaacaa ttgcgggcca aagaccagga aacccaacgc aaagagagaa
     2101 gccaggtaga tatattcgtt gctggcgtgt tgatacacac cgagcgtggc gataatcagc
     2161 gccagcgcga tacaggccac caggccacgg cgaccgttcg ccaggtcaga gagccagccc
     2221 cacagcagcg taccgaccag cgcaccagct tcaaacagcg taaagctctg aatcgccacc
     2281 gctttagaga gtttcagttc ctggaacgca tacacggttg accactggtc gataccaata
     2341 cgcaccacat agaggaaaat gttggcgaag cacagcagcc agatcacttt gtttttcagc
     2401 acatactcaa caaagatctg ccacttggtc atatcggtag attctgtctc tttgtcctct
     2461 tcgctgatct cctcgccgaa cagctcttca gctttgccga ggccataaga ttccggggag
     2521 tcgctgccgt aacgcaggcc gataaaaccg acaatcagcg cgataatcga cgggaaaata
     2581 aacatgccga tgacatgacc atcgaacagg taatttgcgc cgaacagcgc cacacctgct
     2641 gcgcctgcac caccaaggtt gtgagaaatg ttccagaaac cgaggaatgt cccgcgttta
     2701 cgacgcggcg tccatttggt gatggtggag taactgcacg aaccgccggt actctggaaa
     2761 aagccgctca aggcgtagaa ggcaatcatc aggaacaggc taaccgagcc gctgcccata
     2821 ctggcactga agcccagcat acaaatggcg gacaggatca gcatgaacgg caggaattgt
     2881 ttggtgtttt tgccgtcggc gtagtaagaa accagcgttt tacccacgcc ataagtgatg
     2941 gagaagccca ggccgatcat ccccagctgc gtcatgctca acccgtaggt cgagatcatg
     3001 tcgttctgcg cgatgttaaa gttcttgcga atcaggtaca tcgtaaggta gccgataaag
     3061 accaccaggt aggattgcat gaacggtttg aaccacattt tgcgccgcac ttcgagcgga
     3121 aggtccaggg tcggcttgcg aacctggttt aggaaagcca gcatgggtta ctcctgattt
     3181 cccttcatac ttcaagccgc aggtgcctta gctgcacgca ttcctcccgg tcacatagtt
     3241 cactatgctc ccgggactca cgcgtttgct gcgttcctgc aacttgaatt attttgggaa
     3301 agaattaatg aatgatggca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc
     3361 aggcaaaacc tggaaaattg cttagttttg ccggatatga agtgaaaagg tgatatgcat
     3421 cacgcttcgc gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc ggaaaccccg
     3481 gcggcaatgg cgataaccac aaaaaatccg ctccagtgcc aggtatcgag cactttcgcc
     3541 agcggccaac cagcaagcga cgcccccaga taagcaaaca agccgacaaa ccccgtcgcc
     3601 gcccctgccg cctctttgtg ggaacattct gccgccgcca tgccgattaa catctgcggg
     3661 ccaaagacaa aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat
     3721 ggcatcagcc acagcgagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc
     3781 ggcccgcggt tgccgttaaa caatttgtcc gagccccaac ccgctaccag cgcaccgata
     3841 aatccaccca gttcaaacat cgtcactgcc gtattcgccg tgaccagatc gacgcccagc
     3901 gtctcggaca tatacaaatt gccccagtcg ttgattgccg cccggaccac atagaccagc
     3961 acatagcaaa acgaaagcag ccagatatac ggattcagta acacatattt ggtgaggatc
     4021 tctttacgcg tcagccctgc cccttcttgt tgttgagcta tctccagcgc gtcgtgccgc
     4081 cattcaccaa ccgccggtaa acctaacgcc tgcgggcgat cgcgcagccg ccagcagaga
     4141 aaaatcccca cgactatcgc catacaaccc gcaatcatca tcccggcacg ccagccgtaa
     4201 tgcagcgcgc tcgctgccat cacaatggga atgagtgcgc cgccgacgtt atgcgccgtg
     4261 ttccataatg cccaccaacc gccgcgctcg gtacgagaat accaggcggt taacagacgc
     4321 gcacacaccg gtgaacccca gccctggaaa aaggcgttca acacccaaag cacggcaaac
     4381 gcccatagcg acgtcgagaa gccaaacaga atgttgataa tgcccgtggc aataagccct
     4441 atccccataa aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac
     4501 acgccgtagg taatgtaaaa cagggtcgct aacaggccaa tatcgctacg gctgagcacg
     4561 ccgttagcaa ggatttctgg tacggcggcg ttaaaacttt tacgcgtgaa gtaaaacagc
     4621 gcgtaaccca gccagatggt cagcagaata tgccgacgcc agtagcgata gcgggcatca
     4681 atttcatgtt tatcagtcag taatggcgca tcggcaggcg ctttcagaaa cggcaacatc
     4741 atcgaacctt agacataacg ttgaggtaga gaaacgctga cacgcgtgcc gtgcagacag
     4801 gaaatgtgta atgtgccacc cagcgccgtt acgcgctcgc gcattccggt aaggccaaaa
     4861 ccgtgttgcc cggaatctgg cggtaaaccg ctgccatcgt cttcaatcac cagcattaac
     4921 cgctcatcat gctgccagcc ttgcagcgtg accgcgctgg catcggcatg tttcacaatg
     4981 ttgttcaacc cttcctggca gacgcgaaat agcgtcacgc gctggttttc gcttaacgct
     5041 gattcatcga ttcgccattc gagatgactg acaatgccgc gcccttccag ctccatttcc
     5101 cgcatcagtg agcggatggc ctgctccagg gtgagatcat ccagctggcg cggacgtaac
     5161 cgccctaaca agcggcgcac cgcgtcgtaa acgcccagcg atagttgttc gatgagctgc
     5221 ccgctctgtt tcacgctggc gttatccgcc gcgagccgct ggacaatgcc cgcttgagtt
     5281 cgaatagcag taatggtctg accgatatca tcgtgcagct cacgtgccac atcacggcgc
     5341 acgctctctt cggtttccag caaccgttca gcgagatgct gattgcgcgc cagttccttt
     5401 tgcagcgact ggttaagttc acgcaaccgc tggatgcctg cgcccagtaa caaccctgtc
     5461 agactttgca ccagcagcga gagcaataaa tccaccggat gatcgcgcca ggtttgactg
     5521 gcgatcagcg cgatggcgtt catcaacgtc gcaattagcg ccccttgcca gccatagtgc
     5581 caggccagcg cgataatcgg cagcgccagg cagaatggcg taaagcgcga cagttcgtcc
     5641 ggcaatccca actggagcca gagactgata acaaacagca gcaagtacca gaccagatgc
     5701 cgcccacgcc agttgatcgg ttgagaaact aacgacgggc cgagcggcag ccaggtgtta
     5761 ttggcgagat agtgccagaa caccagacat atcggggcca gcgtcaggcc gccagttaaa
     5821 gtcagcaaca gcgcattcca cgactctttg ccgtgccaaa gccagggcag cgactgcaac
     5881 aacgccgccg ccgttaatgc cgccccttgt agcagcaagg tgcgccagtc acgttgatgt
     5941 cgatagcgcg agatcagcgc cacgggcagt aacgtcagta aactaccgat catcaataag
     6001 gagaaatggg ttaaaccgac cgcctgcgtt aaccagtaaa tcagcagcca ctccgcgccc
     6061 agcaacaccg gccagtatcc gcgcgggcat tgcagcatta gccccagacg cagaccaaac
     6121 ggaaataaca gcaccgccat atcagggcgc tcaaccaggt gcaggctgat actccacagg
     6181 caaaaccatg cggcagagaa gataaaaaag caggcaataa cggtaattaa gcgggagaac
     6241 aacgtcttca tcaccaacca tcaaacatgc ggcgcgccag ttcaacgtcg ttactgacgc
     6301 ccagtttttc catcagattg gcgcgatgga cgtgtaccgt tttcggcgac aagcccagtt
     6361 cggcggcaat ctctttcacc gccattcctt gcgccagttt ttctgccacc tggcgttcgc
     6421 gtttggttag cgggtcctga cgaccggatg ccagtttaat ggcaatatcc ggcgtcagat
     6481 aacagccgcc cgtggcaacc gtatgcaccg cagcaatcag ttcgtcaggg ctacaacgct
     6541 tggagagaaa gccgcgcgcc cccgcgttaa gcgcctgctc aaccagcgcc ggactgtcat
     6601 gaacagagag cataatcgtc gccatacctt tcggcagctg gcttagcagc tccagaccgg
     6661 agatatcggg catggagata tcgcaaatac acacctgcac accgcgcccc ggcaaccccg
     6721 ccagcgcctc gcgccccgaa ccaaactcgg caactacctg caaatcaggt tccagcccca
     6781 gcagctgcgc aaagccggag cggacgataa ggtgatcgtc tataagggca acggtgatca
     6841 tgatcttgtc ctggcgggta ataaaacgcg cttaccttaa cgataagcgc gatgttgttc
     6901 aagccttgag cggttactga aaaaacaccg caatcttgtt aaacattgtc ggatcggact
     6961 gattacgctg cactttcacc acatcttcca gcttatcgat ttggcttatc atctgctcca
     7021 gacgctggtc gtcattgacc agtagccaga tatggctttt gtcgctgtcc tgaatcggca
     7081 ggcagagaat gccttcaacg ttaaaagcgc gacgggcaaa aaggccacaa acgtgggtca
     7141 ttacgcccgg atggttgcga acggtgagtt cgagaatcac gttgtcatga gttgtgtttt
     7201 gcatggctta ttcccccacc atttcagtat tcgccgcacc tggcggcacc atcgggtaca
     7261 ctttttcttc ggcatcaatg cgcacgtgga tcagcgccgg accagggcga ttgatgattt
     7321 cctgcaatgc agcctgcgga tcggtttcgt tattcaaatc acaggtttcg aggccgaatc
     7381 cggcggcaat ctgcataaag ttgatttttc ccggataggt agcggcaaaa acgccctgct
     7441 tgtagaacag actctgttgc tgatgtacca gccccagcgc ttcgttgttc atcagaatga
     7501 ttttgacatc cagctgattt tcgctagcgg tcgccatctc ctgaatattc atcatcaggc
     7561 tgccgtcgcc ggagaaacac aacactttgc gatccgggtt cgcaagcgct gcgccaatcg
     7621 ccgcaggcag gccaaagccc atcgtgccca gcccaccgga ggtcagccac tggcgtgggc
     7681 gattgagcgg ataagcctgc gccgtccaca tctgatgctg acccacgtcg gtggtgataa
     7741 tcgcgctgtc atcgacacag gcggcaacag cgttgatcag gccgtaatgc gttaacggat
     7801 cgcacgcttt cgggattgga cacgggaatt cacgctgcaa atccgccacc aactggtgcc
     7861 actctgcacg cggttgcgct tccaccagcg ggatcaactg cgccaacacg tcatcaacat
     7921 ccgcctgaat tgccacatgc ggttgcttaa ttttgcccag ctctgcgcgg tcgatatcga
     7981 catgaataat tttggcattc gggcagaact gctcggtttt gccaatcgcc cggtcatcaa
     8041 aacgcgcgcc gagaacaatc agcagatccg cctcctgcaa aatatagttg gtgctgcgca
     8101 cgccgtgcat ccctagcata cccagcgaca gcggatgcgc ttttggcaac atacccagcg
     8161 ccattaatgt cattgtggta ggcagctgcg ctttctccgc cagttcacgc actctcgcag
     8221 gcgcattgat cacaccaccg cccagataaa gcaccgggcg tttggcagca ttaatcattg
     8281 ccgctgcgtc acgaatgctt tcttcgctaa aggcgggcgc gatggctttt tctgccaccg
     8341 cgggctgtgc ttcaatctca aaaacggccg tttgcacatc cttaggaatg tctatccaca
     8401 ccgggccagg gcggcctgat tgcgcaatac ggaaggcatc gctcatgacc tgcgggagtt
     8461 cttcgatatg tctgaccaga tagttgtgtt tggtgatggg gatagagatg ccgtaggtgt
     8521 cgacttcctg gaaggcgtcg gtgccgatca tcgaggcggg aacctgacca gtgatgcaaa
     8581 tcagcgggat ggagtccagc cgcgcatcgg caatggcggt caccaggtta gtcgcccccg
     8641 gtccgctaca ggccatacag accgccggtt taccgtcggt gcgcgccatt ccctgagcga
     8701 taaaccccgc gccctgttca tggcgagcca gaatatggcg gatttgcgtg ctttggctta
     8761 aggcatcgta aacaggcagg atagaaccgc ccggaatgcc cgtcacaatc ttaatgccct
     8821 gctgttccag gaaatgaacg ataaattctg cgccggtaaa gcgcttacgc gtcgatgttg
     8881 tgcccgaact tgccatgctc cagtcctttt cttctgggcc gactttccgg gaggtgctta
     8941 aacgaaaaac cccgcccggt ttgcgccggc ggggttttgg aatcgtgtgt tgttccagtc
     9001 cctacggcgc attgccgacg accaccacca cacgcacgac gaccactgcg gcggatggcg
     9061 cagttggtag tagttttgcg ttgagcatgg aagtagtcat tggggacctt gttggttttg
     9121 tgtttaacaa tatttataca agcacagctt tacaggggag acaatggaaa atttttcagc
     9181 aagggaaaat tgaggggttg atcacgtttt gtactgaatt gcag
//
LOCUS       DAZGHF010000167         9187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGHF010000167 DAZGHF010000000
VERSION     DAZGHF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.168"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            342..770
                     /gene="uspC"
                     /locus_tag="ACHK3A_004350"
     CDS             342..770
                     /gene="uspC"
                     /locus_tag="ACHK3A_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO8742355.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(777..2201)
                     /gene="otsA"
                     /locus_tag="ACHK3A_004351"
     CDS             complement(777..2201)
                     /gene="otsA"
                     /locus_tag="ACHK3A_004351"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO8742356.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2176..2976)
                     /gene="otsB"
                     /locus_tag="ACHK3A_004352"
     CDS             complement(2176..2976)
                     /gene="otsB"
                     /locus_tag="ACHK3A_004352"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO8742357.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3143..4129)
                     /gene="araH"
                     /locus_tag="ACHK3A_004353"
     CDS             complement(3143..4129)
                     /gene="araH"
                     /locus_tag="ACHK3A_004353"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO8742358.1"
                     /translation="MSSVSTSGSGAPKSSFSLGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4144..5658)
                     /gene="araG"
                     /locus_tag="ACHK3A_004354"
     CDS             complement(4144..5658)
                     /gene="araG"
                     /locus_tag="ACHK3A_004354"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO8742359.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRTHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     ADQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5728..6717)
                     /gene="araF"
                     /locus_tag="ACHK3A_004355"
     CDS             complement(5728..6717)
                     /gene="araF"
                     /locus_tag="ACHK3A_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO8742360.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7514..8017
                     /gene="ftnB"
                     /locus_tag="ACHK3A_004356"
     CDS             7514..8017
                     /gene="ftnB"
                     /locus_tag="ACHK3A_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO8742361.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8095..8346)
                     /gene="yecJ"
                     /locus_tag="ACHK3A_004357"
     CDS             complement(8095..8346)
                     /gene="yecJ"
                     /locus_tag="ACHK3A_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO8742362.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8461..8547)
                     /gene="azuC"
                     /locus_tag="ACHK3A_004358"
     CDS             complement(8461..8547)
                     /gene="azuC"
                     /locus_tag="ACHK3A_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO8742363.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8810..9133
                     /gene="yecR"
                     /locus_tag="ACHK3A_004359"
     CDS             8810..9133
                     /gene="yecR"
                     /locus_tag="ACHK3A_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO8742364.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         2385 a         2309 c         2099 g         2394 t
ORIGIN      
        1 catctgctat aaataattgc tttaatgcaa taccagataa gctattttta aacagacact
       61 taccgcacaa caaactaatt aaatacaaag aaaggcaaca atcaacttaa tgttaataaa
      121 atgttttaca tatattaacc acttgtttaa tatggagaat cagtacatta ttttcttata
      181 attacagttg aaatcatatg atcaccgaga gtgattgatt aattgtattt actaataatc
      241 acgctaatat ttacctgttt gactgagttg ggattgcgtc gtttctccat taggagtaaa
      301 gctttaatgt cacctgaagt tcacagaata aggaacaggt tatgagctat agcaatattc
      361 ttgtcgctgt tgcggttaca ccggaaagtc agcaactgct ggcaaaagcg gtatctatcg
      421 ccaggccagt aaagggacac atcagtttaa ttactctcgc ttccgacccg gaaatgtaca
      481 atcaattagc tgcgccgatg cttgaagatt tacgcagcgt gatgcaggaa gaaacgcaaa
      541 gctttcttga taagttaatt caggatgcgg ggtatcccgt tgacaagacg tttattgcct
      601 acggtgagtt aagcgaacat attctggaag tttgtcgcaa atatcatttc gatttggtga
      661 tttgcggtaa tcacaatcac agtttctttt cgcgagcatc ctgctcggcg aaaagagtga
      721 ttgcctcaag tgaggtcgat gtgctgttag ttccacttac gggagattaa ccgctcctac
      781 gcaagcttag gaaaggtagc aactttatcg cgctgctggc tttccgcgct tcgcggaact
      841 atctgcttta ggtcgctaat gaagcactcc tgccagtggt taatatcgtt tttcacgata
      901 acgtccagca tttctgcatg acgggaaata cgttccgcca gcgacatagt caatgcacga
      961 tccagcgcag ctgcaacttc gtcacgatcg tagggattaa caattaacgc cgacgttaac
     1021 tcgtttgctg ctcccgcaaa ttgcgaaaga acaagtacgc ccgggttggc tgggtcctga
     1081 gcggcaacat actcttttgc caccaggttc atcccgtcac gcagtggcgt cactaagccc
     1141 acgtcagagt agcggaatat tttcatcagt aatttacggt caaaatgctg attcaaataa
     1201 taaagcggcg tccagcctaa ttgcccgtat ttaccattaa ttcgtccagc ttcattttcg
     1261 agctgatgac gaatatcctg ataggcttgc acatcaccac gcgacgttgg tgcaatctgg
     1321 gtataacgaa ttttaccatg atgctgcgga tatttttcca gcaacgcttc ataggcgaga
     1381 aaacgctccg gcaaaccttt ggaataatcc agccgttcga cagaaaagat attttgtacg
     1441 tttttcagtt ccgctttaag ttgcgccagt tttggcggca gtggtccggc agcctgtttc
     1501 gctatttctt tcggttcaat gccgatcggg taaacttccg ttcgaaacgc tttgccgcag
     1561 gccgtatggc tttttgcgct acgtgtcgtg acgcgggtca ggttagaaag gcaatccagg
     1621 aacgccagac gatcgttctc cgtctggaaa cccagcaaat cataatcaca aagctgttca
     1681 agcaaggtgt catatgtcgg cagcgcgttg aagatttccg gcgtcgggaa aggaatatgc
     1741 agaaagaaac caatgcgatt attcactccc cgtttgcgta attcatgcgc aaagggcaac
     1801 aggtgataat cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct
     1861 gccagcaacg catttacgcg tagatagcca tcccaggcag gacgctgaaa ttgcaccaga
     1921 tcgagccgat aatgaaaagc gggccagaga acggcattgg agaatttgtt gtagtattcg
     1981 tcaaggtcct gttcgctgag gttaaaagag gcccacgtaa tgttaccctt tttcaccttt
     2041 tttagcggct gatcctcatt ccctgtttca ccgctccagc caaaccacag tccacctgcg
     2101 gctttcagtg cccccagtat gccaacggca aggccaccgg cactggcggc gtgctcgtct
     2161 ggtggtgcaa tccggttaga tactacgact aaacgactca tagtcatcac tcctgttatt
     2221 ttctcttttt tgttgtaatg cggtggttat catttcaagc cagctccaga catccggcac
     2281 gcccgccagt cgccatgatg cctgagttgc gcctgtgcca atttttactg acattccgcc
     2341 cagccggtta acgactgcga agccagattc atcggttaaa tcatcgccca gaaatacagg
     2401 cgttcgcccg ataaagggag cttcctgcat aaaggcagca atcgcctcac ctttactggt
     2461 gcctcgcggt ttgatctcga caacacactt tccctgctgt aacgccattt gtggccagat
     2521 ctgagtaata cgttgcgcta atgtcattaa tgcgtcttca tgctgcggag cctgacgata
     2581 atgcagcgca aaggccatcc ctttcgtctc cagctccgca ccgggaaact gggcgatagc
     2641 cgtatgcagt tgcacgctaa tatcacgcgc aatcgcatcc ggcagatgaa cgatatgcgt
     2701 tttaccattg atgtcacggc gctccgcccc atgcacgccc gctaacggga agcgataagg
     2761 ttttgccagt gcgtcaagct ccaccattga gcgccctgaa atcaatgcca atgcaccatc
     2821 acttgcggtt gccagtagct gaagtccttg cagaatagtg tcaggcacga cgacctgatc
     2881 gggatgcggt ttgatttccg ccagcgttcc atcgagatca aaaaaccagg catatttcgc
     2941 ggatagttca ggggtttcgg ttaacggttc tgtcacccgg ttctcctcct tctcttttct
     3001 tattgtgttg cgcagaacgc gctttacaga catgtaagta tagacagtgt gacgggggtc
     3061 gccatttgca acgaagaaca gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgc
     3121 taaattgttg tggaaaaaag catcagacag tgcgtttcgc tttttgcttg taacggtcga
     3181 aaatcaccgc tgccagcagg attaagccgc gaactacgta ctgtgcgaaa ggagaaatat
     3241 taagcaggtt catggcgttt tccacggtgc ctaaaattaa gatacccgcc accacatatg
     3301 agatttttcc gatgccgcct ttcagagaaa cgccacctaa aacgcaggcg gagatcacaa
     3361 tcagctcata gccaatcgac gtcattggct gcccactggt catacgtgaa gccagaataa
     3421 ttccggctat cgctgatacc aggccagaga gaacaaagat aataattttg gtgcgaacaa
     3481 ccggtacacc cgccagacgc gcggcctctt cgttcccgcc aatcgccagg gtattacgcc
     3541 caaaggtggt tttattcagc agcaagccaa agatgatcag acacgcgacg gtgagccaga
     3601 ttggcgcagg cagaccgaac cagttggcgt aaccaagggc aaagaagctt tcatcttcga
     3661 taccgaccgc tttaccgtct gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg
     3721 ccagtgtcgt gatcagggca tttattttca gtttggcaat aacaaagcca ttgaccaggc
     3781 cacagagaac gcccagcaac aaacccgctg ccacgccaat ccacaggctt tcagtcaggt
     3841 taataaccac cgccgtggtg acacccgcac aggcaattac ggaggcgaca gaaaggtcaa
     3901 agtcaccgga agcaaggcag aacaacatgc cacaagccac catccccgac atggaaattg
     3961 ccagacccaa ccctttcata ttaataaagg tggcaaagtt cgggacaaaa atggcacagg
     4021 cgataaaaag gaccgcaaac accaccagca tgccgtactg atcccagata cgccctaagc
     4081 tgaatgacga cttaggtgcg ccagaacccg atgtagaaac agaagacatc atactctcct
     4141 tactcaggca actgcctggc tgactttagg catcgcaagg ctcagtgcct gacgctcatc
     4201 tgcctgctcg tgtaacaatt caccggcgat ttcgccttcc cgcatcacca caatccggtc
     4261 ggcaacgccg aggacttcag gtaagtcgct ggaggcaaac agcaccgcca caccctgcgc
     4321 cgccagcgca taaattacgt tatatatttc gtgcttagca ccaacatcaa tgccgcgcgt
     4381 aggttcatcc agcaaaatga ccttcatctc ttccgataac cagcggccca gaatggcttt
     4441 ttgctgattt ccgcctgaga gattcatgat cagttgatca gcgcccggcg ttttgatgtt
     4501 gagcgaacga atgtggtgat cggcattgtt ttcttcccaa ccgttgttga ttacacaacc
     4561 gccgagcaca tgtgtacgtc tggcactgat gttgatattg tcgcgaacgg agtgcacggg
     4621 aataatgcct tccgctttgc gatcttccgg gcagagcatc atgcctgcgg caatggcgtg
     4681 gctcggttta cgaatatcga tcggctgttg gtcgatataa acctgaccgg cggtgatttg
     4741 cgtaccgcca aacatgcctt tcattaattc gctacgccct gccccgacca gaccaaacag
     4801 accgacaatt tctccactgc gaaccgccag acttattggc gtacgcacgc ctggtgcttt
     4861 cacagcatca agacgcagcc gctcctcgcc ataactacgc ggttgccaac cgtagatatc
     4921 gccgatgtcg cgcccgacca tcgcctgcac cagggcgtcg tggtcaacct gctgcatatc
     4981 ggtaaaggtt ttgacataac gtccatcttt aaagacagtg atggcatcgc tgagggcaaa
     5041 tatttcttcc atacggtgag aaacgtataa gatcacccgc ccctcttttc gcagttcacg
     5101 aataacgcgg aaaagattgt cgatttcacg ggcagagagg gagctggttg gctcatcaaa
     5161 ggcgataatt ttggcattac gcgccagggc tttggcaatt tcaaccatct gccactgacc
     5221 aatggagaga tatttcagcg gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg
     5281 taaacccgcc tcataattca gcaatgagcg attgacaatg ccgcctttat gcggcagctg
     5341 gccgagatag atgttttccg cgacggtcat ttccggcacg agatgcagct cctggtaaat
     5401 aatcgccacc cccgcgttaa gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt
     5461 aatcactaca gaacccgtgg ttggcgcata gtttccgctg aggattttta agagagttga
     5521 ttttcctgcg ccattttcac ccatcaacgc atgaacctga ccggcatagc agtcaaaact
     5581 aatatccgtc agcgccttaa cgccgggaaa cgttttaccg atgccgcgaa atgagagata
     5641 cggggtagac tgttgcataa cgtctccgtg aatcactggt cgttcacacc ccctctgcat
     5701 catgcagagg ggaatctttc cggcaaatta cttaccgcct aaaccttttt tctccagttc
     5761 ttctttaaag ttgtcacgcg tgatcagcac cacgtcggta acttcggtaa attttggcgg
     5821 ttcaacgtct tttgctaccc agttgtaaag catttcgctg gacttataac catgtacgtc
     5881 cgggcttggc agcagggaac cgtagaagcc ggttgcctgt gccttagaca gttcgctcac
     5941 cgcatccaca ccgttaatac cgataccgat gatatcggcc gctttaaagc cctgaccttc
     6001 cgtcgcgcgt acgccgccca gcacggtgct gtcgttcata ccgacgatca gccaatgttt
     6061 aacttccgga tgttgaacca gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt
     6121 agatttggta ggtacctgat aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc
     6181 catagaaccc gttgtacggc ggcgggcggt atccagttcg ttggcggtaa tcgccatcac
     6241 cgcgctttct ttgacatccc agccacgttt ctgcatctct ttatacagtt cctggccctg
     6301 acgttcgcca attttggtcg cagccatcat caccagcgga acggtatcca ttggcttacc
     6361 tttggcgtta acaaactggt catccacggc aatgactttc atatcgtagc cacgcgcttt
     6421 cgccacgatg gcagagccga gtttcgggtc cggagtacaa ataacgaaac ctttcgcgcc
     6481 actggcagcc aggctgtcga tagcgttcaa tgttttttcg ccatccggca cggcaatctt
     6541 aataacttca aaccctaaat ctttcccggc tttatcggca aacttccatt cggtctggaa
     6601 ccacggctct tccggttgct tcaccaggaa accgagcttc aggttctccg ccatagcgga
     6661 ttgtgacata acggctgcca gaccaatggc tgccagggct ttagtaaatt tgtgcatggt
     6721 tctctccagc tttagtgtcg ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa
     6781 tcggaaaaca aagcattacc ttttaactaa aagataagtg actgtgttga catagtttta
     6841 gcgagaaatt aattctccat aggagagcaa tatcacatcg cagaattaca gtgagaacgt
     6901 gcataaattt agcgggaaaa gacataagga aaagcgaatt tgtcagacaa attatcgagt
     6961 gcacagcaga ataatccata agattagcct ggaagtcctt gttgtctttg gtacccatgc
     7021 gggatgtctt ctttttaacc agtcaatagg ccgcattacc tggcgttgag tttttgcaat
     7081 ggtgtaataa ccacaactca aagatgtgga aaatgtgcgt cattcatttc gccattaatt
     7141 atcactgtgc tcattaatta acagaacacg tataatgaga gccatctcgc aaaaatgaaa
     7201 aaacgtttta taaaaccatc acttcatcat gaattcaaat taatcgatta atatcaacaa
     7261 gatacaaaaa gcactatcat taaaattcat tgcagttaca ttgatttcat caatgaaatg
     7321 taaaaatata taaacttgat gatttaagca ttttcttata cccgttcaga cgttattctt
     7381 atttcagatc atcgtcagaa ttgactccac gatcacattt cggaccggca gaaaggaatt
     7441 attctgcaaa cagtaattat ggtgttttga tttatcttgc acctctccac ttctggatat
     7501 aaggatatta ggtatggcaa ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg
     7561 cgagttttac gcatccaatc tctaccttca cctgagtaac tggtgttctg aacagagtct
     7621 gaacggcacc gccactttcc ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg
     7681 catgtttaac tttatgaaga gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc
     7741 cggtgaaaaa ctgaactctc tggaagaact gttccaaaaa acgatggaag aatacgagca
     7801 acgttctagt acgttggcac agttagccga tgaagcgaaa gaactgaatg atgattcaac
     7861 cgtcaatttc ctgcgcgatc tggaaaaaga acagcagcat gatggcctgt tgctgcaaac
     7921 cattcttgat gaagtgcgca gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca
     7981 acatgttctg aatgtcgtgt cgcatcagct acattgatca tcatcggcgc taatgcattg
     8041 cgccgatgaa gtttttgaga aaccgctgcc tcattttttt gaagcagcgg ttttttaatg
     8101 ggattcgccc tgtggggtaa acttgagttc aatcaacgcg atggcttttt ggattgcccg
     8161 catggtgacc gggtctgcgg cggcgggatg gttagtaaag tcgatattct ttagctggct
     8221 ggacattttt tcgcgaactt caacgggtgc gatcacatcg agaacatcca gaatttgttt
     8281 gataaccaac tggcaagcaa ccacatcaga aaccagttcc tgatcggcat tcagcggctg
     8341 ggacatcgta aactcctgat agcattttga aagccgttat agtagcggct tcacatcttc
     8401 agcgatagtc acatccaccg tcatcaggac acaaaaaaac ctgccggagc aggttttttg
     8461 ttatcggaac atattgcctg gcggtacgtc tttgaacgtc ttgcaatagt tattgaacat
     8521 acttttcagg attttgcgca gtttcatcgc ggcactccga ccatttgtta tacaggtgtt
     8581 attgtctttg cgcttataat gtgacaacca tcacaaaaat caatctttat gtgatgcaaa
     8641 tcacataaat acccctttaa tgttataaaa atgataatca aaaagcagct accccatttc
     8701 tgacacctac agatggcaag aaatagcgcc tgccaggcgt cttttccggc cattgtcgca
     8761 gcgctgtagc gcgtaaaata gtgctttctt ttactcttct ggctggacca tgagacttct
     8821 gattctgact ctttcattaa taacgctggc ggggtgtacg gttactcgcc aggcccatgt
     8881 gagcgaagtt gacgcggcaa caggtattgt tcggttggtt tatgatcagg cttttttgca
     8941 gcatgcccgt accgatcgtt atgtcagtcg cgggatcgcc gatcgcgcat gccagcaggc
     9001 aggctatacc cacgctatcc cctttggtca gccagtaggc aactgtagcc tctttgcggg
     9061 ttccttatgt ttgaatacgg aattcacttt gtcatatcag tgccatcatt ccgccttccc
     9121 tgtctttctc taaacgtttt aatcagcgag ggggatcttc gctgattaaa taaatagcgg
     9181 taatgcg
//
LOCUS       DAZGHF010000168         9141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGHF010000168 DAZGHF010000000
VERSION     DAZGHF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.169"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            48..410
                     /locus_tag="ACHK3A_004360"
     CDS             48..410
                     /locus_tag="ACHK3A_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742365.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(513..971)
                     /gene="ybbJ"
                     /locus_tag="ACHK3A_004361"
     CDS             complement(513..971)
                     /gene="ybbJ"
                     /locus_tag="ACHK3A_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO8742366.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLSAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(968..1885)
                     /gene="qmcA"
                     /locus_tag="ACHK3A_004362"
     CDS             complement(968..1885)
                     /gene="qmcA"
                     /locus_tag="ACHK3A_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO8742367.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2031..2708
                     /gene="fetA"
                     /locus_tag="ACHK3A_004363"
     CDS             2031..2708
                     /gene="fetA"
                     /locus_tag="ACHK3A_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO8742368.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2695..3474
                     /gene="fetB"
                     /locus_tag="ACHK3A_004364"
     CDS             2695..3474
                     /gene="fetB"
                     /locus_tag="ACHK3A_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO8742369.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3537..4391)
                     /gene="cnoX"
                     /locus_tag="ACHK3A_004365"
     CDS             complement(3537..4391)
                     /gene="cnoX"
                     /locus_tag="ACHK3A_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO8742370.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4452..5261)
                     /gene="ybbO"
                     /locus_tag="ACHK3A_004366"
     CDS             complement(4452..5261)
                     /gene="ybbO"
                     /locus_tag="ACHK3A_004366"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO8742371.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHCGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5251..5874)
                     /gene="tesA"
                     /locus_tag="ACHK3A_004367"
     CDS             complement(5251..5874)
                     /gene="tesA"
                     /locus_tag="ACHK3A_004367"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO8742372.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5845..6531
                     /gene="ybbA"
                     /locus_tag="ACHK3A_004368"
     CDS             5845..6531
                     /gene="ybbA"
                     /locus_tag="ACHK3A_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO8742373.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6528..8942
                     /gene="ybbP"
                     /locus_tag="ACHK3A_004369"
     CDS             6528..8942
                     /gene="ybbP"
                     /locus_tag="ACHK3A_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO8742374.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2092 a         2340 c         2503 g         2206 t
ORIGIN      
        1 gataaatctc aatttttaag aggtttacgc caggcaggaa aatttaaatg aatgaatttg
       61 agaagatttt taatgaaatg aatctggacc gggcattgtt accgatcctt tttcgttcga
      121 accggtcgac agtgtggaag tatttaagcg gcgatagtac ggctcctgcc tctgccatgt
      181 cgctgatcat gctgttgcag ttgatccaga aacgaaaccc cgatctgtta gccgaatggt
      241 taacgctttc tgatttcacc attccgccag aagtttacct ggaccaacct gattactgga
      301 aagggtgggt gtatacccaa cacaaggtca ataaaaacgt cctggaatat ctaaaaaaac
      361 actatccgga tgaagaccaa aaaagtatgg gtaaaggccg ggaagagtaa ttttgcctct
      421 actcggctat atgccggatg cggcgcaagc gccttatctg gcctacgatt cgggcatatt
      481 ttgtaggcat gataagacgc gccagcgtcg catcaggcga tgaccgcacg gataatcagc
      541 gttatccctt ctatcgcaat cacctcaaca tgcgtacctg cactgagatc ctcgctggcg
      601 ctgacaggcc atgaactgtc accgacgcgc atatgaccac gcccgttgac cagcggagat
      661 tccagaacaa aacgtcggcc aatcaactgc tgcccgcgct ggtttaaatg actgtcgctg
      721 tgcttttgtt cgcgcacccg ccgcgacaac catttccacc acagccaggc ggcgagcagc
      781 gtcaggacgg caaacattac cccttgccac tcccaaccca gcggcagcaa ccagaccacc
      841 agaccggtaa tcaccgctgc cacgccgctc cacaacagat aaccattgcc gcccaacatc
      901 tcggctgcca gcagcaaacc gccgagactt agccagaaaa tatgtggatg aacgaccatt
      961 aactccatca tggctgagtc cgtttgttgg cgctgtcttt caccagctca gcaatcccgg
     1021 caatcgaccc catcaggctg ctggcctcta atggcatcat caccactttg ctgttactgg
     1081 acgaaccgat ctgctgtaac gcttcggtgt atttctgtgc gacgaagtag ttcaccgcct
     1141 gaatatcacc ggaggcaatg gcttcagaca ccattttagt ggcgcgggct tctgcttcag
     1201 cggaacgttc acgcgcttca gcctgtaaaa acgccgactg acgttcgcct tccgctttca
     1261 ggatttgcga ctgtttttca ccttcggctt tgaggatttc cgcctgacgg atcccttccg
     1321 cttcaaggat gtaagcgcgt ttggtacgtt ccgctttcat ctgcgcgttc attgaagaga
     1381 taagctctgc cggtgggcgc acgtcgcgaa tttcaatacg ggtgacttta atcccccacg
     1441 ggttggtggc ctcatcgaca atacgcagca ggcgtgagtt gatgctgtcg cgctgagaga
     1501 gcatttcgtc aagttccatt gaacccagca cggtacggat gttagtcatg gtcaggttga
     1561 tgatcgccag ctccagattg ctgacttcat aagccgcgcg cggcgcgtca atcacctgaa
     1621 taaagcacac ggcgtcgatg gtaacgttgg cgttatcttt cgagataact tcctgggaag
     1681 ggatatcgag cacttgctcc atcatattga tcttgcgacc aatgcgatcc ataaacggca
     1741 ccaccagact gagccccggc tgtaacgttt tggtatagcg accaaaacgt tctactgtcc
     1801 actgatagcc ctgcggcacg attttgacgc ccgcgccgac aatgaccagc gcgacaaaaa
     1861 tgagaatcgg gataaagata agcatcggaa aaacctcctg ttgtaccgtc cataatcagc
     1921 aaaattgctg cttgattaaa caaattatac ctgattactg aaagagagtt cccccttatt
     1981 cctgcgaagg ataaactgtt tttagtaaaa atcagaaaaa gggaacagtg atgcaggaaa
     2041 atagtccttt gcttcagcta caaaacgtag gatatctggc gggtgatgcg aagattctta
     2101 ataacatcaa tttttcgctg cgtgctggcg aatttaagtt aattaccggt ccttctggtt
     2161 gtggcaaaag tacgctgcta aaaatagttg cttcattgat cagcccaacc agcggaacgt
     2221 tactgtttga aggtgaggat gtcagcacac taaagccaga aatctaccgc cagcaagtct
     2281 cttattgcgc ccagacgccg acgctgtttg gcgacacggt atacgataat ctgatctttc
     2341 cctggcagat ccgtaaccag cagcctgacc cagccatttt tctcgatttt ctcgaacgct
     2401 tcgccttacc ggacaccatt ttgacgaaga atatcgccga gctatctggt ggtgaaaaac
     2461 aacgcatctc attgattcgt aacctgcaat ttatgccgaa ggttttattg ctggatgaaa
     2521 taaccagtgc gctggacgaa agtaataaac ataacgttaa tgagatgatc caccgttatg
     2581 tgcgcgagca aaatattgcc gtgctgtggg tgacacacga taaagacgaa attaatcatg
     2641 cggataaagt gattacactg caaccgcatg ccggagaaat gcaggaagca cgctatgaac
     2701 tcgcataata ttactaacga atcattagca ctggcattga tgctggtggt ggtggcaatc
     2761 ttaattagcc ataaagaaaa actggcgctg gagaaagata ttctctggag cgtcgggcgc
     2821 gcgataatcc agctgattat tgtcggctat gtgctgaagt atattttcag cgtggatgat
     2881 gccagcctga cattattgat ggtgttattt atctgcttta atgcggcgtg gaacgcgcaa
     2941 aaacgcagta aatatattgc taaagctttt atctcatcgt ttattgccat tacggtcggg
     3001 gcgggaatta ccctggcggt gctgattctc tcagggtcga ttgaatttat cccgatgcag
     3061 gtgatcccta tcgccgggat gatagccggc aacgccatgg tagcggtggg gttgtgttac
     3121 aacaatttag ggcaacgggt cattagcgaa cagcaacaga ttcaggagaa actgagtctt
     3181 ggcgcgacgc cgaaacaggc ttcagcgata ttgattcgcg acagtattcg cgcggcttta
     3241 attccgacgg tcgattcagc aaaaacggta ggcttagtga gtttaccagg aatgatgtcc
     3301 gggctgatat ttgccgggat tgatccggtg aaggcgatta aatatcagat tatggtgacc
     3361 tttatgctgc tctcaaccgc cagcttgtcg accattattg cctgctattt aacctatcgt
     3421 aagttttaca attcgcgcca ccaattggtg gtgacgcaat tgaagaagaa atgatgcgat
     3481 gccggacgcg cagcatcgca tccggcgttg tggttcatgt gccgatcaac ggaaactcaa
     3541 tacaacaacg cgtacagctg gcggcgatac ttcgacgcca gtgcatcacc cgtacccagc
     3601 gcagcgagga tctcctggaa tgttttacgt gtctgaccat ctgcggcggt gagatcttta
     3661 cgcagatgcc caaacagtaa ctccagcgcc tcttcattgc gcccaacctg gtgcagttgc
     3721 agtgccagtt gtgtcgccag tgcggcatct tctggattct ccgccacctg ctgttgcaac
     3781 tgttgaattt ccggcgtatc agccgcctgc ttcagcagtt cgatttgcgc caccagcccc
     3841 tgatagcggg tgtcctgatc ctgcaacgga atggttttca gcacggcttc tgcatcttca
     3901 gaacggttca gcgcaatcag cgtttctgcc agcagcaggc cgatctcccc gttctgattc
     3961 gacaactgcc aggcgtcttt cagcaatggc aaggcgtcgg tgtaattgcc ttcctgcatc
     4021 agctgcatcg cctgctgcgc tttcagctct tcttcgcgcg gcagcacttt atccagcagg
     4081 gcgcggatcg cctcttccgg ttgcggcccc tggaagccat ctaccggttg cccgttctgg
     4141 aacagataca cggtcggaat cgcgcgcaga ccaaactggg cggcaatcat ctgctccgcg
     4201 tcgcagtcca gcttcgccag aataaattgc ccgttgtact gcgccgcgag gctttccagg
     4261 atcggggtta actgcaaaca gtgctggcta cgttcagacc aaaaatagaa cagcaccgga
     4321 gtggtcatcg actgttcaag aacctgttgc aggttagatt cgttaatgtt gacaatattt
     4381 tctacggaca tggagtcgct ctctgttgtc gatattttct ttgacatggg ggcttaagcg
     4441 cgcgcttcaa ctcacccctg caatattttg tccatcacgc gccccggcag caggcgctta
     4501 agcaccatta ccgcccaggt caccagcgtt accggatagc gcatcttcgg cttctcgcta
     4561 ataaaagcat ggcgtacttt gtccaccacc gcttccggtc ccaacgtaaa gcgggcggcg
     4621 atgccgggat tttcgactgg tttatcactt tgcgtctggt tgacgttgtc ggtgaagcga
     4681 gtacgaatgg gaccgggttc gatcaggctg actttaattc cacagtggcg tagctccatg
     4741 cgcagcgcgt ctgaccacgc ctccagcgca tatttactgg ccgcgtaagc accacgaccc
     4801 ggcgtggaga ttaatcccat caccgatgag gtcatcacaa tacgcccttc accgtgcggt
     4861 aacatcgcgg gtaacaggcg catggtgagc tggtgtgcgc cgaaaaagtt ggcggaaaac
     4921 tgctgttcca tctgcgcacg gctgatggtg gaaagggggc catacatgcc gaatccggca
     4981 ttgttaaaga tcccatacag acaattatcg gtcagggcga tcacctcgtc ggctgcgcga
     5041 tcaacacttt ctggtgaatc cagatcgatc aacacgccgg taaatcccat gctgttcatg
     5101 cgctcaacat catccggttt ccggcaacct gccagcacat gaaaaccctg gcgttttaat
     5161 tcgagcgcgc tttccaggcc aattccactg gaacatccgg taattaagac cgatttttgc
     5221 ataactttac ctgtcaggat ctccgttgct ttatgagtca tgatttacta aaggctgcaa
     5281 ctgcttcgcc atccagtcgg caataaacgg ctgggcgtcg cggttgggat gaataccgtc
     5341 atcctgcatc cattgtggtt tgaggtagac ctcttccata aaaaagggca gcagcggaac
     5401 atcaaactct ttggcgagtt tggggtaaat ggcgctaaag gcttcattat aacggcgacc
     5461 atagtttgca ggcagacgta tttgcattaa caatggttca gcgttggcgg ctttgacatc
     5521 ctgcaatatc tggcgcagcg tttgctcggt ttgttgtggc tgaaaaccac gcaaaccgtc
     5581 attgccgccc agttctacca gcacccagcg cggctgatgc tgtttcagca gagccggaag
     5641 gcgcgccagc ccttgttgcg aggtgtcgcc gctgatgctg gcattgacta ccgacgtttt
     5701 actctgccac ttatcgttca acaaggcagg ccaggccgcg ctggcagaca ttcgataccc
     5761 ggcgctcagg ctatcaccca gaatcaataa cgtgtccgct gcggcggcac ggaaggttaa
     5821 caggaccagg aacaggaagg gcaaatgcca gcggaaaaca ttgttgaagt tcatcatctt
     5881 aagaagtccg tcggtcaggg ggagcatgaa ctctccatcc tcaccggagt tgagctggtt
     5941 gtcaaacgtg gcgagaccat cgcactggtg ggcgagtcgg gatcgggtaa gtcaactttg
     6001 ctggcgatcc tcgccgggct ggatgacggc agcagtggcg aagtgagtct ggtgggacaa
     6061 ccgctacata atatggacga agaagcgcgg gcaaagttgc gcgcgaagca cgtcggcttt
     6121 gtttttcagt catttatgtt aattcctacc cttaacgcgc tggaaaacgt cgagcttccg
     6181 gcgctgctgc gcggtgagag tagcgcggaa agtcgtaacg gggcgaaagc gttgctcgaa
     6241 cagttagggc tgggtaaacg tctggatcat cttccggcac agctttccgg cggtgaacag
     6301 caacgagtgg cgctggcacg agcctttaac ggtcgacctg atgtgctgtt tgccgacgaa
     6361 cccaccggca atcttgaccg acagacgggc gataaaattg ccgacctgct attttccctc
     6421 aaccgtgagc atggcaccac gttgattatg gtgacccacg acctgcaact ggcggcacgc
     6481 tgcgaccgct gcttacggct ggtgaacggg caattgcagg aggaagcatg attgcacgtt
     6541 ggttctggcg cgaatggcgc tcgccgtcgc tattaattgt ctggctggcg ctaagcctgg
     6601 cggtggcctg cgtgctggcg ctgggcaata tcagcgatcg catggagaag ggcttaagcc
     6661 agcaaagccg ggagtttatg gcgggcgatc gggcgttgcg cagttcacgc gaagtgccac
     6721 aagcgtggct ggaggaagcg caaaagcgcg gcctgaaagt cggcaagcag ctgactttcg
     6781 ccacaatgac ctttgcaggc gacacaccgc agctggcgaa cgtcaaagcg gtggatgata
     6841 tctacccgat gtatggcgat ctgcaaacta atccccctgg cctgaaaccg caggcgggca
     6901 gcgtattgct ggccccacgc ctgatggcac tgcttaacct gaaaacaggc gacaccatcg
     6961 acgtgggtga tgccaccttg cggattgccg gagaagtgat tcaggaaccg gattccggtt
     7021 ttaacccctt ccagatggct ccgcgtctga tgatgaatct ggcggatgtc gataaaaccg
     7081 gagccgtgca gccggggagt cgggtcacct ggcgctataa attcggcggc agcgagaacc
     7141 agctcgacgg ctatgagaaa tggttgttac cccagcttaa acctgaacaa cgctggtacg
     7201 gtctggaaca ggacgaaggc gcgctggggc gatcgataga acgttcgcaa cagttcctgc
     7261 tgctttcggc gcttctgacc ttgctgctgg cggtggcggc ggttgcggtg gcgatgaatc
     7321 attactgtcg cagtcgctac gacttggtgg cgatcctcaa gacgctgggg gcagggcgag
     7381 cgcaactgcg taagttaatc gtcggtcagt ggttgatggt gctggcgctt tcagccgtta
     7441 ccggtggtgc gataggcctg ttgttcgaaa acgtgttgat ggtgctgctc aagcccgttt
     7501 tgcctgctgc actaccgcca gccagcctct ggccgtggtt gtgggcgctg ggcaccatga
     7561 cggtcatctc gctgctggtg gggctacgac cgtaccgctt gttgctggca acgcagcctt
     7621 tacgcgtatt acgtaatgat gtggtcgcga acgtctggcc gctgaagttt tatctgccga
     7681 ttgtcagtgt ggtggttgtg ctgctgctcg ccggattaat gggtggcagc atgctgcttt
     7741 gggcggtgct ggcgggcgcg gtagtactgg ctttgctgtg cggtgtgctg ggctggatgc
     7801 tgctgaatgt acttcgccgc atgacgctga aatcgctacc tctgcgcctg gcggttagcc
     7861 gcctgttacg tcagccgtgg tcaacgttaa gccagctttc ggcattttcg ctctccttta
     7921 tgctgctggc gctgttgctg gtattgcgtg gcgatctgct cgaccgctgg cagcagcagc
     7981 tacctccaga aagcccgaac tactttttaa ttaacatcgc cacagaacag gtagcaccgc
     8041 taaaagcgtt cctcgcggaa catcagatag tcccggaatc gttttatccg gtggtgcggg
     8101 cgcggctgac ggcgattaac gataagccga cagaaggcaa tgaagatgag gcgctcaatc
     8161 gcgaactcaa tcttacctgg caaaatacgc ggcccgatca taatccgatt gtcgccggta
     8221 actggccgcc aaaagccgat gaagtgtcga tggaagaggg gctggcgaaa cgcttaaacg
     8281 ttgccctcgg tgataccgtg acttttatgg gcgataccca ggagttccgc gctaaagtga
     8341 ccagcctgcg caaagtggac tgggaaagtc tgcggcctaa tttctatttt attttccctg
     8401 aaggggcatt agacgggcaa ccgcagagct ggcttaccag tttccgctgg gagaatggca
     8461 acggcatgtt gacgcaactc aaccgccagt tccctactat tagcctgtta gacattggcg
     8521 cgattttaaa acaggtcggt caggtgctgg agcaggtaag tcgggcgctg gaggtgatgg
     8581 tggtgctggt caccgcctgc ggtatgttgc tgttgctggc acaggtgcag gtgggaatgc
     8641 gtcagcgtca tcaggagctg gtggtgtggc gcacactcgg tgcggggaaa aaactgctgc
     8701 gtaccacgtt gtggtgtgag ttcgccatgc ttgggtttgt ttctggcctg gtagccgcaa
     8761 ttggtgcgga aacggcgctg gcagtgttgc aggcgaaagt gtttgatttt ccgtgggagc
     8821 cagactggcg attgtggatt gtgctgccgt gcagcggtgc actgctgctg tcgcttttcg
     8881 gcggctggct gggtgcgcga ctggttaagg gtaaggcgct gttcaggcag tttgcgggat
     8941 gatgagagta aataaggggc gttctggttg aatggaacgc cttgtttaaa ttaattctac
     9001 aatatgtttc attgatgatg ttattgaatt ggtgatttcc tatctttcta ttgctgatat
     9061 taatctgaat cggtggagtt taatgatttt aaatatagcc ctacaaaaat aactcagact
     9121 aataaaatca taaatcatat g
//
LOCUS       DAZGHF010000169         9134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGHF010000169 DAZGHF010000000
VERSION     DAZGHF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.170"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..182
                     /locus_tag="ACHK3A_004370"
     CDS             <1..182
                     /locus_tag="ACHK3A_004370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742375.1"
                     /translation="RLPEWPEERLHDLLPFSAYRFAELTDVTVIPPNAGKRVMEVVSS
                     TPMNDTASEVKIPTQ"
     gene            482..2038
                     /locus_tag="ACHK3A_004371"
     CDS             482..2038
                     /locus_tag="ACHK3A_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO8742376.1"
                     /translation="MHQYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLFITLILAT
                     LFWHTPVQDLGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMNILRNVLAAVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLAAFNILLPFVIVAIIGEGLKAIRGVGFMTL
                     MCGVSTLIPQYLVAVYLGAELPAFAGSLVSLIVIVFMAKNRKEKTAPEFLIETSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTVKSTVSQIASSLSFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGLIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            2088..2807
                     /locus_tag="ACHK3A_004372"
     CDS             2088..2807
                     /locus_tag="ACHK3A_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO8742377.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQNSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGARLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLSREGHPVKVMHIAEVLMHR"
     gene            2818..4233
                     /locus_tag="ACHK3A_004373"
     CDS             2818..4233
                     /locus_tag="ACHK3A_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO8742378.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSLAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREEATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNGVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFREVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKELPYACSLCTACNGVCPVKIPLAQLIQKHRQVMAES
                     EITPSTERRITRIFNYVNSHPALWKVGMIAGAKTASLCIRNGKTPISPGAIKEWSVAR
                     DLPSPDGESFRSWFKRHKKGG"
     gene            4238..4933
                     /locus_tag="ACHK3A_004374"
     CDS             4238..4933
                     /locus_tag="ACHK3A_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO8742379.1"
                     /translation="MENREEFLVAIARKLGRPVRRIPEAMPEPVSTLATTRLTELTMD
                     QRCEAFIKFASEVMLAKCVLVSPEDAPSKALDICWTFGSGPVIISNDQRLFDTGIAPL
                     LQKEMGAVLWTPEKGEENIHLAEKAKTGVVFAEYGLTESGGVVLFSAPERGRSVSLLP
                     EASVFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDC"
     gene            complement(5113..8229)
                     /locus_tag="ACHK3A_004375"
     CDS             complement(5113..8229)
                     /locus_tag="ACHK3A_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO8742380.1"
                     /translation="MILQTHTIAETSNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     QNQGYEFISVKSQSAMLANVREQLQSLNGVVFNDSEWRRFTEQYLDNPGDGILDKTRK
                     IHIDYICDFIFDNGRLENIYLIDKKNLMRNKVQIIRQFEQAGSHSNRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFRRYYQFGFTGTPVFPENASGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSRGFNAMLAVSSVDAAKVYYATFKRLQEEAAN
                     KSANYKPLRIATIFSFAANEEQNAIGEISDETFDTGAMDSSAKEFLDAAIREYNDHFQ
                     MNFSTDSKDFQNYYRDLAQRVKNQDVDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRNLEQATIDAITLFGDKNTRNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVSELEQRFPAPASIESEKEKKDFVKLFGEYLRAENVLQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQMIRLPAERKIQDYRSAYNDIRDW
                     QHREKEADKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHSRQNKSKDEMTVEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDTFFTFAQREQQREADALIKEE
                     NLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKISAFIEK
                     FKGVGGHL"
     gene            complement(8349..9134)
                     /locus_tag="ACHK3A_004376"
                     /pseudo
     CDS             complement(8349..9134)
                     /locus_tag="ACHK3A_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293189.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         2365 a         2010 c         2127 g         2632 t
ORIGIN      
        1 cacgtctgcc tgaatggccg gaagagcgct tgcacgactt actaccgttc tcagcctacc
       61 gattcgcaga attaactgac gttacggtca taccacctaa tgcagggaaa cgagtcatgg
      121 aggtcgtcag ctctactccc atgaatgaca cagcctctga agtaaaaata ccgacgcagt
      181 gaaaccagcc ttaagctttc caaaacacaa ttacaacata gttgaacaaa gttcattcaa
      241 acaggttctt ttaataacat ttagtcaaat aaaataacaa taaaacgatt cagcaatcat
      301 ttttcataac aaaaacaaat tataaccccc ttccctgttt gtggacgaga ctcgttctct
      361 ccctgcgaga caagcgtaaa ttggtcacac cgacggtagt tgcctgaaaa ttacaaattc
      421 tcgtctgaca gggcaataac aacagtaatg atgctgaatt tgttttcgtt gaggaaatga
      481 catgcaccaa tacctgcttt ttttactggg acttacccca cttctggcaa tgatcatttt
      541 gattctgaaa gtcaaaatgc ccatacatta tgccgtactc agcacactct ttattacact
      601 gattcttgca actttgttct ggcacacacc agttcaggat ctgggttctg ctgttgtata
      661 tggcgcaatc aaagggctgt ggccgatagt gatagtcata cttgctgcca tttacagcta
      721 taacctgatg aatgcgacaa aaagcatgaa cattctgcga aatgttctgg ccgctgtcag
      781 tgatgataaa cgtatccagg tgcttcttat ctcatggtgt tttggtggat ttcttgaggc
      841 ggcggccggg tacggtacag ctgtagcaat ccccattggt atactcattg cactgggctt
      901 taaccctctt aaagccgcaa ttgcatcatt ggttgctaat acagtaccta ccgcgtttgg
      961 tgctgtcggc atacctgtat cagtacttgc tgaacaggta aatttacccg taacaaccct
     1021 cagcgggacg attattcttc agctggcagc gttcaacatt ctgcttccct tcgttatagt
     1081 cgctattatt ggggaaggcc tgaaggctat acgaggcgtt gggttcatga ccttaatgtg
     1141 cggtgtgagc acattaatcc cacagtacct ggtggctgtt tatcttggcg cagaacttcc
     1201 tgctttcgcg ggaagtctgg tcagcctgat cgtgatagtc tttatggcaa aaaaccgtaa
     1261 agaaaaaaca gctcctgaat ttcttattga gacttcagca gatagcgcca gcgcactttc
     1321 atactctcgt ggggcgttgt tacgtgcgtg ctcaatctac atcctgattt ttctgttcat
     1381 tcttctttgc tctccactgt tcccgacggt aaaaagtaca gtgtctcaga ttgcctcgtc
     1441 actcagcttc tctctgagta acggcagcaa tctgtcgatg aagattgaat ggatcgcaac
     1501 ccccggtgtt ctgattatca ttgccagtgt tattggcggc ttgatacaga aagcatccct
     1561 gtcatgcctg ttaaatgtat ttgtcagtac ggtaaaacag ctcaaaaact ccattattgc
     1621 catttctgcg attgttgcta tggcaacggt aatggatgtt tgtgggttga ttggcactct
     1681 ggcacagagc cttgtagatc tcactggtgg ggcctacctc ttcattgctc cggttatcgg
     1741 cgctcttggt acatttgtta ctggcagtga cacaaactcc aacattctgt ttggtaaatt
     1801 gcagaccact gcggctgaaa aattgcatgt ggacccactc tggctggctg ccgctaacac
     1861 atctggcgca acaggcggca aaatgatttc tcctcagagt attgctatcg ctgtttcagc
     1921 cacccgtatg gatggtcagg ggagcgtaat catgtcaggg acactgaagt actgctttgc
     1981 ttatattgtt attcttggac tgaaagtagg ccttgtctac cacctgttca tggcctgaga
     2041 aaaaataaca aacggacgtg gaatattaat caacaggaga aatatctgtg aatgttaact
     2101 ttttcgtcac ctgcattggt gatgcattta aatcccgtat ggcacaaaac agcgtgctgt
     2161 tactggagca attgggttgc aatgtccatt tccccgaaaa acagggatgt tgcggtcagc
     2221 cagcgataaa tggcggttat gttcgtgatg ccattcctgg tatgaaaaat ctcatatctt
     2281 cgctggaaga gaatgactgg ccggtagtat ctcctgccgg gtcatgcact tacacgataa
     2341 aaaattacgc tcgctatctc agtgaagaac ctgagtgggc ggcacgagct gaaaaagtag
     2401 ctgaacgcct gcatgatttg acgtcattta tagtaaatca tcttggcatc acagatgttg
     2461 gggcaagact caatggtcgc gcagtgtacc atccttcatg cagcctttcc cgtaaactgg
     2521 gtgtaacccg agaaccgctt ttgttgcttg agaatgtacg gggacttgag ctgctgccgt
     2581 ttcacgctca ggaaacgtgc tgcgggtttg gcggcacgtt ctctgtaaaa atggcggaga
     2641 tctctggtga aatggtgaaa gagaaagtcg ggcatattat ggatgttaaa cctgattttc
     2701 ttatcggggc tgacgccagt tgcctgctca acatcggcgg tcggcttagc cgtgaagggc
     2761 atcctgtaaa agtgatgcat attgctgaag tcctgatgca cagatgaggt aaacagaatg
     2821 tctttgaaaa caagtgatat caggtttaaa gagcgtatcc gtattcagat tcaggacccg
     2881 atcatgcgaa aagctgtggc taacgcacag gagcgcatag ggactaatcg acagaaaatg
     2941 gttgatgaac tggggcactg ggaagaatgg cgttcacttg ctgaacagat tcgtcggcat
     3001 gttctggaaa atcttgatgc ttatttgtat caattgtccg aaaaagttac tgcgaatggc
     3061 ggacatgtat attttgcccg taccagagag gaggcaacca gttacatact gaaggtggcg
     3121 aaagaaaaaa acgccaggaa ggttgttaaa tccaaatcaa tggtgactga ggaaattggt
     3181 atgaacggcg ttcttcagaa agccgggata ccagtgatag aaacagatct tggtgaatat
     3241 attcttcagc tggcgcatga gccgccatcg catgttgttg tgccggcaat acataaagat
     3301 cgccatcaaa ttcgcagagt tttgcatgaa catctggggt atgacggccc tgaaactcca
     3361 gaagcaatga cattgttcat ccgtgagaaa atcagacagg attttctgag tgctgaagtt
     3421 ggcgtaacag ggtgtaactt tgccgttgca gaaacaggct cagtttgcct tgtgacaaac
     3481 gaaggtaacg cacgtatgtg caccacgtta cctaaaacac atattgctgt gatgggtatg
     3541 gagcgtattg caccaacatt ccgggaggtg gatgttctta tcaccatgct tgcaaggagt
     3601 gctgtgggag caagactgac aggttataac acctggctta caggtccaag agaagaagga
     3661 catgttgacg gcccggaaga gttccatctc gtcattgttg acaatgggcg ttcagaggtt
     3721 ctcggctcag aattcagcga tgtattgcgc tgtattcgct gcggtgcctg tattaatacc
     3781 tgtcccgctt atcgccagat tggtggacat ggctatggct caatctaccc gggcccaatc
     3841 ggagcagtta tttccccgtt gcttggtgga tatgaagact ttaaggaatt accttatgcc
     3901 tgctcattgt gcactgcctg caatggggta tgtccggtaa aaatacctct ggcacaacta
     3961 atacaaaaac atcggcaggt tatggcagag tccgaaataa caccatcaac agaacgccgt
     4021 ataaccagaa tattcaatta cgtaaacagt catccggcat tgtggaaagt tggcatgatt
     4081 gctggcgcga aaacagctag cctgtgcatc aggaacggaa aaacgccgat tagcccaggt
     4141 gctattaaag aatggtctgt ggccagagac ttaccttctc ctgatggaga aagcttccgt
     4201 agttggttca aaagacataa aaaaggaggg taactttatg gaaaacagag aggaatttct
     4261 cgttgctatc gccagaaagc ttggcaggcc agtaagacgt attcccgaag caatgccgga
     4321 gcctgtaagt acgctggcta cgacacgttt aaccgagttg acaatggacc aacgctgtga
     4381 ggcttttatt aaatttgcct cagaagtgat gcttgcgaag tgcgttctgg tttcgcctga
     4441 agacgcacca tcaaaagcat tagacatctg ttggacgttc ggttccgggc cagtcattat
     4501 cagcaacgat caacgactgt ttgatacggg gattgcacca ttgctacaaa aagagatggg
     4561 cgctgtcctg tggaccccgg agaagggtga ggaaaatatt catcttgcag aaaaagcaaa
     4621 aacaggcgtc gttttcgctg agtatggact gacagaatct ggtggtgttg ttttattctc
     4681 cgcccctgaa cgagggcgtt cagtaagtct tttacctgaa gcgtctgtgt ttgttttacg
     4741 caaaagtacc attcttccca gagtggctca gcttgcgcaa aaacttcatg atatggctca
     4801 ggaaggacaa cgtatgcctt cctgtatcaa tctgattgga ggcccaagtt ctactgcaga
     4861 tatagaactg atcaaagttg ttggtgtgca tggacctgta catgcaactt acttgattat
     4921 tgatgactgc taatacattt tagtatgtat atatgattaa cagctccgtt tagctgagct
     4981 gttaatcatt catttagttg tattagtcgt gacttgagcg taagcgtcaa tacggactcc
     5041 gttaatgcct tacaatagca gtcagtgttc atgcctgttt attgaggcaa gggggagtcc
     5101 gggcgcctgt aactaaagat gcccccccac acctttaaac ttctcaataa atgcgctaat
     5161 cttctgaaaa acggtctgtt ttttcgtttt atattgcggg tttaacggac taagttttgg
     5221 taatgtctcg tttaattctg tgccattttc ggtggcgtat tcgcgtttta acgacgtgcg
     5281 aatatagcgc ctggccgctt cttcattaag attttcttct ttgatcaatg catctgcttc
     5341 acgttgttgt tcgcgttgag caaaggtaaa gaacgtatca atgatgctgg ctttgtccgg
     5401 caaatcatca aggttcgttt gctgaataaa atcgaccacc aggccttctt tagcacggtt
     5461 ccccagactt gaacgaatta atcgtttgac ctctacggtc atttcgtcct tacttttgtt
     5521 ttgtctgctg tgttcgaaaa tcaggccaag gatataatcc aggtttattt cctgagactt
     5581 cagcaaatcg acctcaaaaa ccacgtcatc ccagtcagtg gttgattttt cttttttgtc
     5641 agcttctttt tcgcggtgct gccagtcacg aatatcgtta taggcagaac gataatcctg
     5701 aatcttgcgt tcagcaggga gacgaatcat ttgcaattca gcgaactttt catcatccac
     5761 ataatgttct gctttgaatt tttctaccgc aacaggatcg ctgagatcga tttgttgcag
     5821 ggctttcagc gtggcaaatt catcatagtt ttgcaggacg ttctcggcac gcaggtattc
     5881 accaaacagt ttaacgaagt cttttttctc tttttcgctt tcaatactgg caggggcagg
     5941 gaacctttgt tccagttctg aaactactgc cataaagccg cgtttagctt cgccagtggc
     6001 agcatctgta aagccttcca tatactctgc ataactcttt tccagcacca catttctggt
     6061 gtttttatca ccaaaaagtg tgatggcatc gatggttgcc tgctccaggt tacggaatgt
     6121 gacgatgtta ccgaaggttt tcgtggcgtc ataaatgcgg ttggtacggg aaaacgcctg
     6181 catcagaccg tgaaaacgca ggtttttatc gacgaacagc gtgttcagtg ttggagcatc
     6241 gaagccggtt aaaaacatcc ccacgacgat taacaggtca acatcctgat ttttaacccg
     6301 ctgagccagg tcccgatagt agttctgaaa atccttgcta tcagtactga aattcatttg
     6361 aaaatggtcg ttatattcac gaattgcagc atccagaaac tctttagcac tgctgtccat
     6421 tgcgccggta tcaaaagttt catcggaaat ttcaccaatg gcattttgtt cttcattggc
     6481 ggcaaaggag aatattgtcg caatacgcag tggtttataa tttgcagatt tattagcggc
     6541 ttcctcctgt aagcgtttaa acgtcgcgta atagactttc gcagcatcca cgctgcttac
     6601 tgccagcatg gcattaaaac ctctggaacc cgggaaagtg cggtgggtct tctggcggaa
     6661 gttattcagg atatattgcg tgatttcctg aatacgcatg ggatgaagaa acgcctgctg
     6721 attttcagcc gcactcagtt ttttctcgtc agtttctgtc tctaaagact taaactgtgg
     6781 ccgcacatcg ttgtagtcca ccttgaattt gagcactttt tcgtcacgaa tcgcatcggt
     6841 aatcacatac gaatgcaatt cacgaccaaa tacgctggcg gtcgtttctg aacctgaggc
     6901 gttttccggg aaaacaggtg tgccggtaaa accaaactga taatagcgtc tgaatttctt
     6961 cttcagattt ttctgcgctt ctccaaactg gctgcggtgg cattcatcaa atataaacac
     7021 cacttgctga ttgtatacag gcaggtcgct ttctgctttc atcaggttat taagtttctg
     7081 aatagtggtg acgataattt tgttatcgtc cttatccaga tttcgtttaa ggcctgcggt
     7141 attttccgag ccgttaacac tatctggcga aaaacgctga tattccttca tggtctggta
     7201 atcgaggtct ttcctgtcga ccacaaagaa gactttatca ataaagtcca gttctgttgc
     7261 cagacgcgcg gctttaaagc tggtcagtgt tttaccggaa cccgtggtgt gccagatata
     7321 cccaccgctt tccggttttg accagttctt cgctgtaaag gagctcttaa ttttccacag
     7381 aatgcgctcg gtggcagcaa tctggtacgg tcgcatcacc agtagcgtct gactgctgtc
     7441 aaaaacgctg tagttcacca gaacattaag cagggtatgt ttctggaaaa aggtggcggt
     7501 aaaatctttg aggtctttaa tcagcgtgtt gtctgatttc gcccagttca tggtgaagtc
     7561 aaaactgttt ttatcgcgct ttgtcgtgtt ggcaaagtaa cgggtatcgg taccgttgga
     7621 aatgacgaac agttgcagat acttaaacag ggaattttcg ctgttaaaac tctccttgct
     7681 gtaacgatgt atctggttga aagcctcacg aatcgccacg ccacgctttt tcagttcgat
     7741 ttgcaccagc ggcaaaccat taaccaggat agtgacgtca taacggttac tgtgagaacc
     7801 tgcctgttca aactgccgga taatctgcac tttgttgcgc atgagattct ttttatctat
     7861 cagatagata ttctcaagac gcccgttgtc aaaaataaag tcacagatat agtcgatatg
     7921 gattttacgg gtcttatcca gaatgccatc gccagggttg tccagatact gctccgtgaa
     7981 acgccgccat tcgctgtcat taaacaccac gccattgagg ctctgaagct gttcccggac
     8041 attagccagc atcgccgact gtgattttac ggaaataaat tcatagccct gattctgcag
     8101 gtcctgaatc agttcacgtt ccaggtccga ttcactctgg tagctgtcgc ctgtttgctc
     8161 agccttgatg tacttatcaa gaacgataaa gttactggtt tcagcaatgg tgtgtgtctg
     8221 aagaatcata gcgcatcctt tgtgccgtct ggcggggccg gaagggagtt aagggtgact
     8281 tccggcgtgt aaaaatggtt tatatactga ccggatgtta aggtggcccg gtcggtagca
     8341 attgtcaatt agttactgac ggtttcaggt ttcgggaaac tgaacagtaa atcacgatag
     8401 tactcgtatt gtttctggcg caactcgatt tcacgcggga gaccttcggt gatggagttg
     8461 gtcagggtgt cgaatttatc gagtatttcg acaatgcgag cttgttcttc tgaaggtggt
     8521 acaggtattg ggaattttaa aaacattggt cgacgaagtg aggtaaccga tgaacttact
     8581 gatgcttttt gaatataggt aaaaaaatat gctgttatat agtgatatac aaatcgcgga
     8641 ttcataaatg catttgggac aatacgatat gctctctgat gcaaagcata tttaccttta
     8701 gcgtaatgaa ataccttccc caccccggca ccatctccag ccgtaatgat tgctgtttca
     8761 tcaaaatcaa atacatttaa ctttaatggt tcacgtccac gtgcataaaa aatatatttg
     8821 ccatgttcta tagcatcttg cgtatcatgg ttacctgttc ctataacagc cacttccccc
     8881 aaagtcttcc actcaatccg aataccatcc atggcatccg gaaggatgta attacccccc
     8941 ccgcgcaata tctttgaaat tcaataactt atctagatag tggttgtatt gttttttacg
     9001 catgttaagc tctgctgtca gctctgctgt cagggcgctg aatttgtcca gaattcgcac
     9061 gatttcagac tggatggcaa gggatttttc cggattgtcc gggcagggga ttggaacttc
     9121 aaacttatct gtat
//
LOCUS       DAZGHF010000170         9084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGHF010000170 DAZGHF010000000
VERSION     DAZGHF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.171"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(187..768)
                     /locus_tag="ACHK3A_004377"
     CDS             complement(187..768)
                     /locus_tag="ACHK3A_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8742381.1"
                     /translation="MALLGYARVSTSHQKLTHQISELKNAGVREDRIFTDMMTGATDE
                     REGLQRLLARAEKDDVIICTKMDRLGRNTADMIRVVDSCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRIAALAAGVKFGRKKHPRTPTALELISQGES
                     LGSVTEKTGISRSTYFRLKRTIKNDAKIATFYK"
     gene            925..3963
                     /locus_tag="ACHK3A_004378"
     CDS             925..3963
                     /locus_tag="ACHK3A_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFO8742382.1"
                     /translation="MQKYKKRISILTKSEINELYQVPSFNPVERDEFFSLDISLKREI
                     DKIINIESRVYLILITGYFRYKPVIPEFTLKDVKRDVKYIIQTYYKNESTNLNLNIPK
                     STKYRLVKRMLSTLGFEQLTPSIKNELSERLNDVATICMEPKYIFDELLAFLGQRRIA
                     LPGYSTVQNLVSDALNFERKRTINILSKRIYPATAQKITSIIHDDGILNSIRGYNYST
                     RDFSASEIENELRTHNVISSIYYEIKDIINSLGISQGNMEYYATIVKHSTTFRLKRHP
                     KWQGILYLCCYLYFRYREINDKIITAFRYLVKKHKDASVLFAKARATEEIDLINKKLK
                     FASKIFNFFVDSNIEDSIPFGYVREKVFSLISKDDMHLVSEFFDKNKIDVIEYQWQYI
                     DSHHLKIANSIRKLFLAIDIVYDNEKSVIGQQITAAKDDLRGKGKLDAKDLRVIHPSE
                     KNYIVKEGEINTQRFEFHMYSRVSNHLENGVFYAAESEQNKRLEDDLIERSYWHKNKS
                     ILIEKTGLERLITPITQTLNELENTFSDKLARITIGINSDANEFVKKQPHSNRLQWSL
                     ASKKWKTSIDNPIYNQIKNIGIVDVMKYVNNETNFLSVFNAISSRKNNLEADYNDLLA
                     CIFGNGANYGVHRMASASDRSIGILRDVNDKFIRPETTGMANTVISDAIANLSVYKYW
                     TINEDSPFGSVDGQKHTCRINTFKARYSAKYFRKGKGVSAMTLVSNHVPLSTVVISPN
                     EYEGHFAFDLLYNNTSEIQPKSLATDNHGINNVNFAILDIFGYQFSPRYAKFKKIFNE
                     LFDVILESDKLIIKLKKQFNFKLIEHEWENIQHIICSLSRKTTIQSTVVKKLSNTKRS
                     NKTLAALREYDRVIKCIYVLDYADNKTLRQFVQQALNRGEAYHQLRRAIASVNGNQFR
                     GGNDYQIDQWNDCARLIANCVIYYNSAILSRLVEKFEKENNKKAIDVLANLSPVAWRH
                     ILLGGNYSFEDQVAITNLDSLLEGVDPLSDIGDIESEYDG"
     gene            complement(4031..4255)
                     /locus_tag="ACHK3A_004379"
                     /pseudo
     CDS             complement(4031..4255)
                     /locus_tag="ACHK3A_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4799..5098)
                     /locus_tag="ACHK3A_004380"
     CDS             complement(4799..5098)
                     /locus_tag="ACHK3A_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8742383.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLAENASLWDARNLTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDEASRVNILAVVGQRQNPRFII"
     gene            complement(5088..5351)
                     /locus_tag="ACHK3A_004381"
     CDS             complement(5088..5351)
                     /locus_tag="ACHK3A_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFO8742384.1"
                     /translation="MNSTTHYENANFLRELAESLPRILPEGSTDKSALLQRLANEELA
                     RAEYDEQVRAKIAAARADKRPGISSAQLRQQLQGRYQELRNEL"
     gene            complement(6420..7277)
                     /gene="repA"
                     /locus_tag="ACHK3A_004382"
     CDS             complement(6420..7277)
                     /gene="repA"
                     /locus_tag="ACHK3A_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO8742385.1"
                     /translation="MADLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDI
                     SEEAVASARRSRVEWENRQRKKQGLDALGMDELIAKAWRFVRERFRSYQAELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            complement(7270..7344)
                     /gene="tap"
                     /locus_tag="ACHK3A_004383"
     CDS             complement(7270..7344)
                     /gene="tap"
                     /locus_tag="ACHK3A_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO8742386.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCG"
     gene            7423..7542
                     /locus_tag="ACHK3A_004384"
     CDS             7423..7542
                     /locus_tag="ACHK3A_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFO8742387.1"
                     /translation="MSKIIGVFAFLAPVHPHQNQFPATLWRGQPQNSLKRSAI"
     gene            complement(7578..7835)
                     /locus_tag="ACHK3A_004385"
     CDS             complement(7578..7835)
                     /locus_tag="ACHK3A_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO8742388.1"
                     /translation="MSQTVNAETSSSGKKRPYRRGNPVPARERQKASLARRSATHKAF
                     HAVIQLRLKEKLSELADEDGITQAQMLEWLIESEVKRRKSL"
     gene            complement(8119..8601)
                     /locus_tag="ACHK3A_004386"
     CDS             complement(8119..8601)
                     /locus_tag="ACHK3A_004386"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8742389.1"
                     /translation="MEINVTAPVLLTDEHILLPFDCGNEVLSNWLRSRAMKNQMFNAS
                     RTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGY
                     GFGKWLLSDAIHRVASLAEQVGIKAVMVHAIDDDARGFYERFGFVQSVVASDTLFYKI
                     "
     gene            complement(8592..8882)
                     /locus_tag="ACHK3A_004387"
     CDS             complement(8592..8882)
                     /locus_tag="ACHK3A_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO8742390.1"
                     /translation="MSTTASIRKAPRENQINIRATDEERAVIDYAASLVNKNRTDFII
                     ELAYQEAKNIILDQRLFVLDDERYHSFIRQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT         2628 a         2007 c         1960 g         2489 t
ORIGIN      
        1 tccgggttac tgtccgcgct gcggtgctga cgccccttct caccaaaaac ttcaacgacc
       61 agccactggc ttccgcttct accaaacaat gccttgcaca aacgtctcac ggtcagcggg
      121 gttaaatctg ggggttcgcg cccatttcct ctaaaagtct caccttgctt aggtaattaa
      181 ttgaatttat ttataaaacg ttgcaatttt agcatcattt tttattgttc tttttaacct
      241 gaagtacgtc gatcgagaga tacctgtttt ttctgtcaca ctgcccagag attctccctg
      301 tgatataagc tccaacgctg taggcgttcg tggatgcttc ttcctgccaa attttacgcc
      361 tgcggcaagt gccgctattc ttccgtcgtt tgtccgctcc agaatacgct cacgttccgc
      421 ttcagctaca gcagcaagga tttggataac cattttgccc attgtccctt ctgtactgag
      481 accgttctcc aggaaccgga tggcgatccc ctttttgtaa cagctatcaa ccacacgaat
      541 catgtcagca gtgttacgcc ccagtcggtc catttttgta cagatgatta cgtcgtcttt
      601 ctctgcacgg gccaacaacc gctgtagtcc ttcccgttca tcagttgcgc cagtcatcat
      661 atccgtaaaa atacgatctt cccgtacccc ggcatttttt agctccgaga tttgatgtgt
      721 tagcttctga tgactggttg atacccgtgc ataccccaat aacgccattt ttagtctcac
      781 ttatcgtttt tgacactgca tggtatcata atgtacaaaa tacgattttt gaaacctgct
      841 tttatgctgt tttagacagg tatcagatat cttaatattt gatactagcc atatttaaca
      901 aatatttagc attttggtaa atgtatgcag aagtacaaga aaagaattag tatattaaca
      961 aaaagtgaaa taaatgaatt atatcaagtc ccctctttta atcctgttga aagggatgag
     1021 tttttttcgc tggatatcag ccttaaaaga gaaattgata aaataataaa tatagaaagc
     1081 agggtgtatc tgatattaat aacagggtat ttcagatata aacctgtgat tcctgaattt
     1141 acattaaaag acgtaaaaag ggatgtgaaa tatattatcc agacatatta taaaaacgaa
     1201 agtactaatc tcaatttaaa catacctaaa agtacaaagt accgccttgt taaacgaatg
     1261 ctttctactc taggatttga acaactcacc ccatctataa aaaatgagtt aagtgaacgt
     1321 ctgaatgatg ttgctactat atgtatggaa ccaaaatata tttttgatga attacttgct
     1381 tttttaggac agcggcgtat agctcttcct ggatacagta ctgtccagaa cctcgtttca
     1441 gatgctttaa actttgaaag gaaaaggaca attaatattt tgtcaaagag aatttatcct
     1501 gccactgcac aaaagattac atccatcatc catgatgatg gaatattaaa ctccatcagg
     1561 ggatataatt atagcacgag agacttctca gcatcagaaa tagaaaatga attaagaact
     1621 cacaatgtga taagtagtat ttactatgaa ataaaagata taattaattc attaggtata
     1681 tcccagggaa acatggagta ctatgcaact atcgtaaaac acagtacaac attcagactt
     1741 aagcgacacc ctaaatggca aggaatactt tatttgtgct gctacttgta cttcagatac
     1801 agagagataa atgataaaat aatcacagcc ttcagatatt tagtaaaaaa acataaagac
     1861 gcttcagttt tgtttgcaaa agctcgggca acagaggaaa tagatctaat aaataaaaag
     1921 cttaagtttg cgagtaaaat atttaacttc ttcgttgata gtaacattga agacagcatc
     1981 ccttttggat atgttcgtga aaaggttttt tcacttatat cgaaagatga tatgcatctt
     2041 gtgagtgaat tttttgacaa aaataaaatc gatgtaattg aatatcaatg gcagtatatt
     2101 gacagccatc accttaagat cgccaattca atccgaaagc tttttctggc tatagatata
     2161 gtatatgaca acgagaagtc tgttattggc cagcaaatta ctgctgccaa ggatgatctc
     2221 agaggaaaag gaaagctgga tgctaaggat ctgagggtaa ttcatccatc cgagaaaaat
     2281 tacattgtaa aagaaggaga aattaacact caacgattcg aatttcatat gtacagcagg
     2341 gtatctaacc atctggaaaa cggtgtcttt tatgctgcgg agagtgaaca aaacaagaga
     2401 ctggaagatg atctcataga acgaagttac tggcataaga ataaatcgat tttgatagag
     2461 aaaacaggcc ttgaacgact tatcacacca ataacgcaga ctcttaatga gctggagaat
     2521 acctttagcg ataaactggc tcgtataacc attggaataa actcagatgc taatgagttt
     2581 gtgaaaaaac aaccacactc caacagatta cagtggagtc tggccagtaa aaaatggaag
     2641 accagcatcg ataatccgat ttacaatcag ataaaaaata tcggaatagt tgacgtaatg
     2701 aagtatgtaa acaatgagac gaattttttg tctgtgttta atgccatatc atccaggaaa
     2761 aataatcttg aagctgacta taatgacctt cttgcctgta tatttggtaa tggcgcaaat
     2821 tatggtgttc acagaatggc atctgcatca gaccgtagta ttggaatact acgtgatgtg
     2881 aatgacaaat tcattcgtcc agaaacaaca ggaatggcca acacagtaat atctgatgca
     2941 attgcaaatc tttctgtata taaatactgg acgataaatg aagattctcc atttggaagt
     3001 gttgatgggc agaagcacac ttgcagaata aatacattta aagcccgtta ttctgccaag
     3061 tatttcagaa aggggaaagg ggtttctgcg atgacactgg tatctaatca tgtgccatta
     3121 tcaactgtgg taatttctcc aaatgaatat gaaggtcatt ttgcatttga cttgttatat
     3181 aacaacacca gtgaaataca accaaagtca ttagcgactg acaatcatgg tataaacaac
     3241 gttaattttg cgattcttga catttttggc tatcagtttt caccacgtta tgcaaaattc
     3301 aagaaaatat ttaatgaatt atttgatgtt atactggaga gtgataaact cattataaaa
     3361 cttaaaaaac aatttaactt taagctcatt gaacatgaat gggaaaacat ccaacatatt
     3421 atttgttctt taagcagaaa aacaaccatt caaagtacgg ttgttaagaa gttatctaat
     3481 acaaagcgta gcaacaagac attagcagca cttcgggagt atgaccgagt tataaaatgc
     3541 atatatgtcc tggattatgc tgacaataaa accttaaggc aatttgtgca acaagcactg
     3601 aacagaggtg aagcttatca tcaactaaga agagcaatag catcagtaaa tggaaatcaa
     3661 tttcgcggtg gtaatgacta ccaaatagat caatggaacg actgtgcgag gttaatagct
     3721 aattgtgtaa tctattataa ttcagcaata ttatccaggc tggtcgagaa atttgaaaaa
     3781 gaaaataata aaaaagcgat agatgttctg gctaacttat ccccagtagc atggagacac
     3841 attctccttg gagggaatta ttcttttgaa gatcaagttg cgatcacaaa tttggacagt
     3901 cttttggagg gagtagaccc actaagtgat atcggagata ttgagtccga atatgacggt
     3961 taacaacatg ataaataatg gtttttatgc taagagtgag acttttggag gaaatgggcg
     4021 cagaacccca atctggatcg ggatcgtatg caatggtagc aaaaataaaa cgcagcattt
     4081 ctgatgtctt gtaacgcttt acagcagctg ccagacgtgc ggcacgttgc ggtgcttccc
     4141 agcggtcgtc actgtaatta gcaacttcag ccagataatc cttttcaagt ttttcagccg
     4201 tgtaacacct gccgcctaaa aaaagccgca tggcctccct ggttttgtct ctcacatatc
     4261 atccctcatt aatgctaatg tatgcataca ttagcattaa tatctgacag caacaagcgg
     4321 cgaagactac ccctaatcaa gtaggggtcg gtttgggaaa cggaaattat tgtacgttag
     4381 gagcattttt taatcgatgc ggataagtaa ctgattgatt catcgtgatc agctatcgcc
     4441 tttaagttat tgcttttctc ttaggggtcc gcttggaatt cggaaaataa accacgttaa
     4501 gactattttt tagtcaaata actttcagaa atcgccagaa tggcgggaca acagcggttt
     4561 tggtgtccga aatcgtacaa ttctgtacat ctacgcaagg aaatgtacgg aagtaagcca
     4621 aatggttatc ggacggtcgg aacatttcgt acagccttta cgttccgtga gttagctggc
     4681 ctggatgtaa ccactcgttc aactcaattc tcttagcgtg agaaaatttc ctttctccaa
     4741 gcggctccca aaccgtaatt cctggcaagt ccggtaagct gagcaccaag ccgggcgctc
     4801 aaataatgaa tcggggattc tgacgctggc cgacaaccgc caagatattg acacgactgg
     4861 cctcatcatt cacttcataa agtacatgct gtccaagtaa gctaatacgg cgaacccctt
     4921 caccatagcg ttcatccgtt tcatacagcc tggggtaatc tgccaggcga tcggttgcag
     4981 tgatcagacg ctctgtcaag ttccttgcat cccaaagaga tgcattctcg gccagatatt
     5041 caaacaacgc cgttatatcc tcaatcgcac ctggtcgcca ttctatttca taactcattg
     5101 cgcagttcct gataacgtcc ctgtagttgc tggcgcagtt gcgcggaact tattcccggt
     5161 cgcttatcgg cgcgagcagc ggcaattttc gctcgtacct gttcgtcgta ttccgcccgt
     5221 gccagttctt cattagcgag gcgttgcaat agtgcagact tgtctgtact gccttctggc
     5281 aaaatacgag gcaggctttc ggccagctcg cgcaggaaat tggcgttttc gtaatgtgtg
     5341 gtgctattca tagttgactc cccccttcat tcaaaaaggg taattgcaga ttttttgata
     5401 ccgcacgcat gagtataacg agtcagagta tcgatattcg ccccggtgat attgtttcac
     5461 aggcaggcaa taaccggaaa aataatgctc atttgcctgg caatctgcgt gttgtcaggt
     5521 tttatgcaga gtatataacc tttcagacac agccaccaac atatgcttat gccgcatcag
     5581 cggcatggaa ggcggcactc tgttgtttca tatgataccg gagtaaaacc gccgaagccc
     5641 ggcgtaagcc ggtactgatt aatagatttc accttaccca tccccaccct gcaaagctat
     5701 acccgctgtc agccatgaca gagcttatgt gcgcggtcgg agtggtcccg acgagggttt
     5761 acccgaagtc ggggcgtatc tccgcgttag cgggccgtca gggccgctta cgagcgtgta
     5821 ctgagaacat ccagccagaa gactgacagc gatgatggat tgtagttaca acattcatga
     5881 ttaaaagcga tgttgttccc gcccttcggg cgggggcggc ggcgcttttc agttatgagg
     5941 gaagggcttt gtggtttcag ttctgcgcgg gaccgggttt tttccggtgc ttgtatttga
     6001 gtgttgtaac taaagtggct ccggtcgggg cccgccgctt gcggtgggag gtgcatatct
     6061 gtctgtccac aggacaggca gtgaacaggt tttcttttta aatggaagta attaagtagt
     6121 ttaaaggaaa tacagtactg gggtttaaaa gaaacattgc accctgtaac gctgacggct
     6181 ggcgctttat gccatgaacg attgtaacct tatggagaat tcccttgcag ttaaatgtgg
     6241 ataagcggaa ttccccgtca gtaagacgtg ttttgcgtga aaaacagtgg gagcatggcg
     6301 agccggaaag cagatgatga ggctgttttt tgtgtgctgc gctgcttttt tgtgcgtcgc
     6361 cggccttcag acgtgcggaa gcctccggca caggccggat tattcagatg aggtaaaact
     6421 cagggggagg cagtggccag ccgtgtgtag ttgttgttcc gcgacattat catgcgttcc
     6481 ttcacgcgac ggtcgatttc gcgcttcacg gcctccaggc ttccccggaa acgcccttcc
     6541 gcgatttcac gcgtcagctg ccgtttcacc agggtgacaa tgtcctggcg ggttctgcct
     6601 gcatcgcggc gcgcacgggc acgtttcatg ccgtgggact taagctctgc ctgataactg
     6661 cggaaacgct cgcgcacaaa acgccaggcc ttcgctatca gttcatccat acccaacgca
     6721 tccagcccct gctttttgcg ctgcctgttt tcccattcca cgcgactgcg ccgtgcggag
     6781 gccaccgcct cttcggaaat atcgagggcg gcaaacagag ccggtgtgaa cgtgatgtca
     6841 gtcggaatat aacagccaat aagcgggtca tattccgtct ggtaggtgat cagtcccagc
     6901 tctgcaagga acgtcagggc ccgggtggcc cgggtgatgg agagttttcc ggcagcagac
     6961 tccgtcgcca gtccgcactc aatggccagt gtggtgatgg aacactggac gcggttggcc
     7021 agcgggtcat agtggaaaca cagcccctgc agcaacgcat caatcgccct gcggcgcagc
     7081 accggcggca tacgccggcg caggccacgc gaacgtgcat acgcaacatg cgcggcaaaa
     7141 tcaaaacggg aggtgaagcc caccgccttt tccatcagtt tttcgcagaa cttcagcgtc
     7201 ccggcacctt cacggggtgt gaataccgga ttcgggttct ttacctggcg gtaatacgtt
     7261 tgttgaagat cagccacacc atcccgcact tacaatgcgc agaaggagtg agcacagaaa
     7321 gaagtcttga acttttccgg gcatataact atactccccg catagctgaa ttgttggcta
     7381 tacggtttaa gtgggccccg gtaatcttct tagtcgccaa acttgtcgaa gattatcggg
     7441 gtttttgctt ttctggctcc tgtacatcca catcagaacc agttccctgc caccttatgg
     7501 cgtggccagc cacaaaattc cttaaaacga tcagcaatct aacatctcat cactgataca
     7561 gcaagaaacg caaatattca caaagatttt ctgcgcttaa cctctgactc tatcagccac
     7621 tcaagcatct gtgcctgagt gatcccgtct tcatcagcaa gctcacttaa cttttctttc
     7681 agtcgaagct gaatgacggc atgaaacgct ttatgagtgg cactccttct tgcaagagac
     7741 gctttttgtc gttctctcgc aggaacagga ttaccccttc tgtaaggtcg tttttttccc
     7801 gatgaggaag tctctgcatt tactgtctgc gacatctagc ctcctctctt ctcagacagg
     7861 gatcgtttcg cagaggatac tacagttttt tgaaatcagc gacttgagaa ttgtgacgaa
     7921 gatctgtgtt ggcgcacaaa tcaactgtga ttactgttgt ttaatgtgaa ttttgttgcg
     7981 aaatagctga aaacaggaaa tgccttaacc tgtgtgccgg ccccgggtgg gaccgatgat
     8041 ttctgaaatg tgaacgccag tccgagggac tatcgattta catcattata aagctacgcc
     8101 taataaaatt gcgtaacatc aaattttata aaacaaggta tcagacgcca caactgactg
     8161 aacaaagcca aaacgctcat aaaaacccct ggcgtcatca tcaattgcat gaaccataac
     8221 agccttaatg ccaacttgtt cagccagact ggcaacccga tgaatggcat cactcaacag
     8281 ccatttaccg aaaccatatc cctgggtaca aacatcgaca gccaggcgcc ctaaaagaac
     8341 aacaggtaca ggattgggca tattatgccg caaactgcgc ccaagctcgg catgtgtgac
     8401 agaaccagta gctaatgagt aataccctac aacacgtaaa gtaccctcca ggcaaataac
     8461 gaacgtgcga gaagcattga acatctggtt tttcatagcc cgactacgca accagttact
     8521 taacacctca ttcccacagt caaatggcag aagtatatgc tcatctgtca gcaataccgg
     8581 cgcggtaaca tttatttcca ttcaggcttt accgccatca gacgttcacg cccttctgca
     8641 ttctgcacag gagcttccag ttgcctaata aatgaatgat aacgttcatc atccagcaca
     8701 aataaacgtt ggtccaggat gatgttttta gcttcctggt aagccagttc aatgatgaaa
     8761 tcggtacggt tcttattgac cagactcgca gcatagtcaa taacggctcg ttcctcatcc
     8821 gttgcccgga tgttaatctg attttctcgc ggtgctttac ggatgcttgc tgtagtggac
     8881 ataattcccc cattggtaac aaactgctat acaataaata ttaccgcaaa tagtaactgt
     8941 tcgctataca aaatcactca cggggaaagt gctccctaat aaaaataata atagatacct
     9001 gaaagttgta acgatgatac gtgtcggcgc acaaatcatg tctgattact gttgtttaat
     9061 gtgatttttg ttgcgaaatg gctg
//
LOCUS       DAZGHF010000171         9031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGHF010000171 DAZGHF010000000
VERSION     DAZGHF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.172"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..613)
                     /locus_tag="ACHK3A_004388"
                     /pseudo
     CDS             complement(1..613)
                     /locus_tag="ACHK3A_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            949..1311
                     /locus_tag="ACHK3A_004389"
                     /pseudo
     CDS             949..1311
                     /locus_tag="ACHK3A_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            1312..1670
                     /locus_tag="ACHK3A_004390"
                     /pseudo
     CDS             1312..1670
                     /locus_tag="ACHK3A_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766597.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            1953..3092
                     /locus_tag="ACHK3A_004391"
     CDS             1953..3092
                     /locus_tag="ACHK3A_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HFO8742391.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDHDALHNLMEYFEYRACDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            3212..4480
                     /locus_tag="ACHK3A_004392"
     CDS             3212..4480
                     /locus_tag="ACHK3A_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFO8742392.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEVISHDNHGKQ
                     PGFLAAISAPLVAIVLLTLRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLIFGVFGLAG"
     gene            4488..5633
                     /locus_tag="ACHK3A_004393"
     CDS             4488..5633
                     /locus_tag="ACHK3A_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFO8742393.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTRIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            complement(6051..6860)
                     /locus_tag="ACHK3A_004394"
     CDS             complement(6051..6860)
                     /locus_tag="ACHK3A_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO8742394.1"
                     /translation="MSGSVMHSQSAAMVPAAYSAGQSAQLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDTWYVSQLQTMVATLKIPLERRNKR
                     TGRTEKARIWEVTDRTARTWIREAVAAAAADGVTFSVPVMPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPEVDAVAMLKNRQV"
     gene            complement(6861..7166)
                     /gene="ccdB"
                     /locus_tag="ACHK3A_004395"
     CDS             complement(6861..7166)
                     /gene="ccdB"
                     /locus_tag="ACHK3A_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO8742395.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGNESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(7168..7386)
                     /gene="ccdA"
                     /locus_tag="ACHK3A_004396"
     CDS             complement(7168..7386)
                     /gene="ccdA"
                     /locus_tag="ACHK3A_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO8742396.1"
                     /translation="MKQRITVTVDSDSYQLLRAYDVNISGLVSTTMQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            7983..8240
                     /locus_tag="ACHK3A_004397"
     CDS             7983..8240
                     /locus_tag="ACHK3A_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742397.1"
                     /translation="MDQEMTFSLSYEQLTRFAERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADSQRLRNLIWPEDDR"
     gene            8240..8830
                     /locus_tag="ACHK3A_004398"
     CDS             8240..8830
                     /locus_tag="ACHK3A_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742398.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQINESTAP"
     gene            complement(8850..>9031)
                     /locus_tag="ACHK3A_004399"
     CDS             complement(8850..>9031)
                     /locus_tag="ACHK3A_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO8742399.1"
                     /translation="LWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLMASFH
                     WWRRKVNRLPPWDDV"
BASE COUNT         2200 a         2375 c         2346 g         2110 t
ORIGIN      
        1 actggccagt gaaatcgtgc catagcggtt ggtggcccca taatatcctg cagccagctc
       61 catcgcacaa tataccgtca tttcttccag ccagtcagcc ggatcgctat gtgcggcagc
      121 agtctgtttc agaatgcggg tgtcgaaccg ggcattaaag ataattactg gttggtctcc
      181 gatcgcatga cgcagttgcc gagccacatc tgtccatgat ggtgcgccgc ggagagcctg
      241 ctcactgata ccatgcacgg cggcggcctg cgccccgatg gagaccgagg gcttcagtct
      301 ggtttcaaat accacctgac cggccgcatc agtaaggccg atttccaggg cttccgcggt
      361 attatccagt cctgtcgttt cagtatccag aaagagcggt gccagactca gccagtcata
      421 tgcctggtgc catcctgcca ctggtgctgt ttagtctgga taaaacggaa agccgctggc
      481 cagccagttg ttgtttactc gtcagtgggc gtttctcacg catcggaaca cagtctgcaa
      541 ttcggtatac agcgaacctg ccactataag tggttttata ccacttaacc ggctcagcac
      601 cgggagctgg cttcgtaccc tgcgtgcatc tgatctgtgg ctggccgtgg cacggctacg
      661 gacagattct gctccgcaac agtatcggcg tgcctgacct cctgaaagtc ccgtaacgca
      721 ttttctctcc tcccatgccc tgacgctatt gagcgcgtta cagaacaatt taggtcattt
      781 aacgagcctc caacggctcg atcgaaataa atcaaatcgt aatcgtcaac gatatatgag
      841 aggagacccc gctcgccgca aatcgatgac cgaacgtagt gagcgaatcg acgaggaagc
      901 ggaagagaac ctgtacgaaa atggagcaat tacgccccac cgtcaagaaa tgagctggct
      961 ctccgcggcg cgccatcata tacagatgtg gctcggcaac tgcgccatgc gatcggagac
     1021 caaccagtaa ttatctttaa tgcccggttc gacacccgca ttctgaaaca gactgctgcc
     1081 gcacatagcg atccggctga ctggctggaa gaaatgacgg tatattgtgc gatggagctg
     1141 gctgcaggat attatggggc caccaaccgc tatggcacga tttcactggc cagtgctgcc
     1201 agccaggccg gactgacatg ggaagggctg gcacactcag cgatcgccga cacacggatg
     1261 acgtcagggg tggtaaacgc tattactgca tatcatcagg aattgctgca gcttatgtac
     1321 tgcgataacc ctgaactcaa ctatgcaaac ctgaaatccc tgcaaaaaca gctggatgaa
     1381 atgtatacct gcatccgtaa taatcatatg atgctcccaa actacggaaa aatgcgccgg
     1441 tatggtgaac cggtttcaac agcgtttgta gagtcgacaa tcaacgaagt gatcgccaga
     1501 caatgaccca aaagcaacag atgcagtggg atagggaagg tacgcattat ttgttacaga
     1561 ccagaaccgc tgtcctgaat aattaactgc aggacaaatt cgccagttgg tacccgggtt
     1621 gcacgataga cgctcagagt ggaggaaagt tatccgcgat ggcagcataa ctacttcccc
     1681 aggttttttc atgctctccc ttaccaatat cctatgacga tcacatcgaa aaaaaacacg
     1741 gaacaggcga acagaaatat aattaattat ttgagtttaa aggaaaaaag aggatacaag
     1801 ctttttgtgg catttttatt tgtataaacc gacaaaaaag aggccgtgct tcccgctttc
     1861 tttgtctgtt tacacggaac atttcaccac tggatacgct aatagatcgg atatgagcac
     1921 atattgcacc cgcctttcat gcagagcata aaatgaacaa gattcacgct aattattacc
     1981 tgcaagattc taccgcacgc cagatagttc aacgcacaat ggggattatt ccgtactcgg
     2041 taaatgtgat gaacgagcat gggatgatca ttgcttccgg ggaagcctca cgtttacatc
     2101 aacgccatga aggtgcggta ctcgcattga ccgaaaaccg ggtcgtcgaa attgattccg
     2161 cgaccgccaa taagcttaaa ggcgttaagc ccggaatcaa tttaccgatt aattttcgcg
     2221 accagctgat tggtgtactg ggcattaccg gcgagcctgg tgaagtgaag gcatatgcac
     2281 agttggtgaa gatggcttcg gaattaatca ttgaacaaat ggctctcctc gagcaaaaac
     2341 agtgggataa gcgttaccgc gaagagctga ttaatcagct gattttacgc gatccgccac
     2401 cggactcact gaatgcggtg gtctcatggt tgggaattaa tctccagctt ccgcgggtgg
     2461 tggtgattat ggaactgcaa caacctgatc acgacgcact gcataatctg atggagtatt
     2521 ttgaatatcg cgcctgcgac catttggtca cgttttccga ctttaatgat ctgataatct
     2581 taaaacctat ctcgctaaaa aacggcgtct gggataaatc gttggaatta agcgatctgc
     2641 agagacttaa acattacgcc cagtcctgtg gtttttcccg actgattgtc ggcggctatt
     2701 tctctggaga aaacggcctg catcgctctt ttcagaccgc gaaatccacc cagtccatgg
     2761 cgaaaagact gaagttgaag catcagttca tttttcatga cgactatgcg ctggcatcat
     2821 tattagatgg gctttctggt acctggcaag cggaagaatt atccaaaatc tggcgcaatc
     2881 tggttgccaa ggacaccaaa ggtatgctgc aaaaaacgct gcagaattat tttgatcata
     2941 attgtgatct ttcgcaaaca gcgactggct tgcatattca tgtcaataca ttacgctatc
     3001 gactgcagag agtggaagat attacaggaa tacaaatcaa taacttaaag cagttattct
     3061 ggctgtatat cggcatggag ttacaacgct gaactgtatt tccctccaaa tttttcgccg
     3121 tctgacgtcg tatttttagc cgatacacac aagtctaacg cggctgcttt ctccatactg
     3181 ccccctgtta ttttcatgga gaaagcagaa gatggtttca ataacagcaa ctggcgccat
     3241 catcgcactc gtggtggcga tcgttttaat tttacgcaaa gtctcgccgg cttacggcat
     3301 gattgtcggt gcgctggtag gcggtctggt cggcggcgcc gatctggtgc aaaccgtgac
     3361 gctaatggtc actggcgcgc aaggcattac caatgccgtg ctgcgcattc tcgcagcagg
     3421 tgtactggcc ggcgtgttga tagaatccgg cgcggcaaac accatcgctg aaactgtggt
     3481 gaaaaaagtg ggggaaagcc gggcgctttt agccttggct attgcgacga tgattctgac
     3541 cgccgtcgga gtatttatcg acgtggcggt tatcactgtt gcgccgattg ccctgtctat
     3601 tgcccgtcgt gccggtttgt cgaaactcgc cattctgctg gcgatgattg gcggcggtaa
     3661 agcgggtaat gttatgtcgc ctaaccccaa tgctatcgcc gtttcggaca cctttcatgt
     3721 tcccctcacc tcagtgatga tgaccgggat aatccccggt ctgttcggcc taatcgtggc
     3781 ttatttcctg gcaaaatgtc tcagccagaa aggcctgatg gtgatgcccg acgaagtgat
     3841 ttcacacgac aaccacggca agcagccggg atttcttgcc gctatcagcg cgccgctcgt
     3901 cgccattgtg ctgctgacgc tacgacctgt tgcgggtatc gtcatcgatc cgctgattgc
     3961 cctgcccgtc ggtgggttag ccggtgcgat ccttatgggc aggtttaagc aaagtaacgc
     4021 ctttatgatg tccgggctta cgcgcatggc gccggtagcc atcatgctgc tcggtaccgg
     4081 tacgctcgcc gggattatcg ccaactcgga actgaaagat gtgctcattc atggactgac
     4141 ctcttccggt ctgccttctt atcttctggc ccctgtttcc ggcgcgatca tgtccatggc
     4201 aacagcctcc accacggcgg gaaccgccgt tgccgccggg gtattcagcc agacgctgct
     4261 ggggcttggc gtatcagccc ttgccggttc cgccatgatt catgctggcg caaccgtact
     4321 tgatcacctg cctcacggca gcttcttcca cgccacgggc ggcagtgtcg gcatgctgat
     4381 ccatgaacgg ttaaaactgc tgccttatga aacccttgtc ggttttgtgg tcgccctgat
     4441 ttccacactg atatttggcg tcttcggact tgccgggtaa agacacgatg aaaatagtca
     4501 ttgcaccaga ttcatttaaa gaaagtttat cggccatgca ggtggccacc gctattgagc
     4561 agggatttcg tgaaatttat cccgaggccg agtatgtcaa attgccgatg gctgacggcg
     4621 gtgagggtac cgtcgaatca atggttgaag caaccgaagg ccattatctg ttccagaatg
     4681 tcaccggacc gctggggacg ccagtacagg cgcgctgggg tatgctggga gatgggaaaa
     4741 cggcgatcgt tgaaatggcc tccgcatccg gactacatca tgttcctcca gaatgccgta
     4801 accctatgct taccaccagt tatggcacag gcgaactgat ccttgccgca ctggaccacg
     4861 gcgttgagcg aattattctc gggattggcg gtagcgccac caatgacggc ggcgcaggta
     4921 tgctacaggc acttggtgtg gcgatgcttg atacaaacgg gaaatcgctt cctttcggcg
     4981 gcggcgcgct aagcaaccta gcgctcatcg atgcctcagg ctgcgacgct cgactgaaaa
     5041 acgtttctgt caccgtcgcc tgcgacgtta acaatccact ctgtggcgca acaggagcgt
     5101 cagccatctt cggcccgcaa aaaggagcaa caccggaaat ggttgcctcc ttagaagctg
     5161 cgctgaccaa cttcggtata cgacttcagg acgcaaccgg acgaaacgtg atgacgacgc
     5221 ctggcgcagg tgccgctggc ggcatgggcg cggcgctgtt agggattctg aatgcggaat
     5281 taaagccggg agtggagatt gttattgacg cgctgcatct cgatagccta gtcgcggatg
     5341 ccagcctggt aatcaccggt gaaggtcgtc ttgatagcca gagcatcagt ggtaaaacac
     5401 cgattggtgt cgcacgctgc gctaagcggt atcagaaacc agtgatcgca ctggcgggcg
     5461 gcttaacgac cgatcaccag atagtgcatg agcacgggct ggacgcggtg ttctccgtgc
     5521 tgacgcggat cgccactctg ccggaagcgc tacaggatgc agaatacaac ctccgcgttt
     5581 cggcacgcaa tattgccagc gtctggaaat tatcacagca gcagatgcgc taattttcag
     5641 tcaggccggc aagcagttgc tggcctggat attctttctc accaggtcca tgctaaaaca
     5701 gcctgcttca gtcatcccac gcaaggcact atcatggcaa aaaaaggagg agagtagtga
     5761 cccccaaaag acggttaagc ggttaaattg aatggtcaat aagggggctg tttgggaaac
     5821 ggaaattttt gtactgtaag ggcattattt aaccaattta agatcctgac gtattgattt
     5881 aacgtcagtg gctgtcgcct gtattgctat atcgcattta ggttatccga ttaaggccaa
     5941 attccctgaa aacgccgtat agcacgggag tacaccctat tgcgacaggc aggtctattc
     6001 aaaaaacagg cttgccgtac aataattctc tatatccaaa ctgcccccaa ttatacctgc
     6061 ctgtttttca gcatggctac cgcatccact tcaggcatcg caaactgcac ccggtgccgt
     6121 gcagccacat ccagtgcaaa caccttcgtg tagacttcag ttgaactgat ggacttatgt
     6181 cccatcaggc tctgcaggac cttcagtggt ataccggcat acagcatgtg catcgcatag
     6241 gaatggcgga atgtatgtgg catgaccggg acagagaacg tcacaccatc agcggcagcg
     6301 gcggcaaccg cttccctaat ccaggtcctg gccgttctgt ccgtcacttc ccagatccgc
     6361 gctttctctg tcctgcctgt tcgtttatta cgccgttcca gaggaatttt cagtgttgcc
     6421 accatcgtct gcagctggct gacgtaccag gtatcggaga gcggaaccag ccggtgagtc
     6481 tgctggccgg caggcattct tcctgccgtc ctggcagctt tttccgtccg ttgtttcagg
     6541 gtcgcaagct gcacaaacgg atacggaggc gcaagcgaaa aatccccgcg cgtcagtgcc
     6601 agtgcttcat taatgcgcgc accggtgttc cacagtgtgg ccagcagcat cttgcggcgc
     6661 agatccggga cgtaatggag cagggcactc acttcggggg ccagcagata tttcggcagt
     6721 tcatcatgaa ccatcgacat ctggcggagt gccagagctg ccggataatc aatggcaaca
     6781 ggaagttgtg cagactgtcc ggcagaatac gctgccggta ccatggctgc agactggctg
     6841 tgcataacgg agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg
     6901 tcattttccc ggtggctgag atcagccact tcttccccga taacggagac cggcacactg
     6961 gccatatcgg tggtcatcat gcgccagctt tcattcccga catgcaccac cgggtaaagc
     7021 tcacgggaga ccttatctga caacagacgt gcactggcca gggggatcac catccgtcgc
     7081 ccgggcgtgt caataatgtc actctggaca tccacaaaca gacgataacg gctctctctt
     7141 ttataggtgt aaaccttaaa ctgcatttca ccagtctctg ttctcatcgg caaaagagcc
     7201 gttcatttca ataaacctgg cgacctctgc catcccttcc tgattttcag cctgccagcg
     7261 ttcagcgcgc agtcgccggg cttcgttctg catagtcgta ctgaccagac cggagatatt
     7321 cacgtcatac gccctgagca gctgatagct gtcgctgtca actgtcactg taatacgctg
     7381 cttcatagca cacctctttc tgacatactt ctggtatgca tataagtata tattctcatg
     7441 cagcaaaaat cagcgcgtta atacacatac tgttatctgg cttttagtaa gccttatgta
     7501 ttttaccgtg cgttatgtta accagtaaaa attaaaacct gacttataaa aacaaagcgt
     7561 aattaccgca ttcccgtttc gtatggatcc gactttattt gaataaacga ctttccggaa
     7621 gtgtaccacg ctgaggaata aatcatattg ctgttttccg cccctgcgcg acgcatcaca
     7681 ggtcagggag atgacacaac atatagtgtc atcagcagct gaatccggca ccacatgttg
     7741 tgcttacagg atgtggcgtt gtgtggaggg agaggaagat cctgaacagc cggaaaccgg
     7801 atctgcgcgg cggggaacgg gcatgcaata tgataatcaa gcaattcaca taaaatcata
     7861 tcagatatga ttttcatcat taaaaataaa acaatatctg atatcatcag aaatcaggag
     7921 tacacaaatc atacaggata tgatttttac ctgtcagaac actatcagat ggagcgtgca
     7981 ggatggatca ggaaatgacg ttttcactca gctatgaaca actgacccgg tttgcggaac
     8041 gacgcatccg ggaatgcaat ctggacagcc agggcgcgat ttatctctgc gagtcagcca
     8101 aagccggagc cgtgctgatc ttctggcatg aactggccat aaatggctat gcctcaatga
     8161 atgccataaa gagacaggag ctcattgacg ccgactctca gcgcctcaga aacctcatct
     8221 ggccggagga tgacaggtaa tgacccagag ccgccgaccg tcgccactgc agcggagagt
     8281 gctgattgtg ctggctgccc ttgatgaaaa acggccaggc cccgtgctga cgcgggacat
     8341 agaacgggtg ctggagcgaa gcggggaagc cccggtctac gggccaaacc tgcgcgcctc
     8401 ctgccgtcgt ctggaggatg ccggctggct gcgtaccctg cgcgctccga acctgcagct
     8461 ggcagtggag ctgacggacg caggccgggc cgtggcacag cctctgctgc tggcagaaca
     8521 ggaccgcctc cgggcagaac agcgtgcggc agaagtcgtc gtactgccgc tgatcccggc
     8581 agcaggtctg cccgcagacg gcacttctgc cactgatctg gcggtggagc tgaacggcat
     8641 aacgtatcag gcctgccgtg gggattttgt ggtacgtctc gacggcagca cctgtctgca
     8701 gttatggaat aaagaaggca gggtggtacg ccgggagggc gatccgctgg aagtggcgca
     8761 gtggctgcag gcctgtcacg atgcaggaat ggaggttcgc gtacagataa atgaaagtac
     8821 cgctccgtaa gaacacgcct gtgcacggtt tacacatcat cccatggtgg cagccggtta
     8881 actttcctgc gccaccagtg gaaagaggcc atgagcagtc cgaacaaaaa accacccgtg
     8941 atacttataa taatggcctc acctgccacc attcctgacg gcccccagta gataaaccac
     9001 attgcacatc cccaggaaat gccccataag c
//
LOCUS       DAZGHF010000172         9006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGHF010000172 DAZGHF010000000
VERSION     DAZGHF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.173"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            274..408
                     /locus_tag="ACHK3A_004400"
     CDS             274..408
                     /locus_tag="ACHK3A_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742400.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     "
     gene            427..681
                     /gene="xisR"
                     /locus_tag="ACHK3A_004401"
     CDS             427..681
                     /gene="xisR"
                     /locus_tag="ACHK3A_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO8742401.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWTQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGVKNASSG"
     gene            715..2001
                     /locus_tag="ACHK3A_004402"
     CDS             715..2001
                     /locus_tag="ACHK3A_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8742402.1"
                     /translation="MSNTSYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGNFDYAAQFPESPNLKHFGLGKKEITIKALSEKWLDLKKIEIGGN
                     AFNRYQSVVRNMLPRIGESRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPVKGRSVV
                     TVNYYMTTIAGMFQFAADNGYTESNPFNGLTPLKRSRTEPDPLTHDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAIETLKSQAEMTRFGTQYQIDVKLREFGRTVSHECTFVFNPQLVKKCQQAGY
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALAAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKELAESVPYMPHRARR"
     gene            2003..2782
                     /gene="yecE"
                     /locus_tag="ACHK3A_004403"
     CDS             2003..2782
                     /gene="yecE"
                     /locus_tag="ACHK3A_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO8742403.1"
                     /translation="MISFSIIELTHGSRHVNCVEGNTTLYALPKPEVVLRWREQTTDD
                     FRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALW
                     HFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHAARPHSE
                     AIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYL
                     FLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2835..3230
                     /gene="yecN"
                     /locus_tag="ACHK3A_004404"
     CDS             2835..3230
                     /gene="yecN"
                     /locus_tag="ACHK3A_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO8742404.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            3271..4014
                     /gene="cmoA"
                     /locus_tag="ACHK3A_004405"
     CDS             3271..4014
                     /gene="cmoA"
                     /locus_tag="ACHK3A_004405"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO8742405.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            4011..4982
                     /gene="cmoB"
                     /locus_tag="ACHK3A_004406"
     CDS             4011..4982
                     /gene="cmoB"
                     /locus_tag="ACHK3A_004406"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO8742406.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(5147..7576)
                     /gene="torZ"
                     /locus_tag="ACHK3A_004407"
     CDS             complement(5147..7576)
                     /gene="torZ"
                     /locus_tag="ACHK3A_004407"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO8742407.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPICSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(7601..8701)
                     /gene="torY"
                     /locus_tag="ACHK3A_004408"
     CDS             complement(7601..8701)
                     /gene="torY"
                     /locus_tag="ACHK3A_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO8742408.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGVLTDPALADRKPLWQYAEKLDDTYCAGCHAPIASDHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2248 a         2251 c         2160 g         2347 t
ORIGIN      
        1 ggctcagtga atgggtgaaa tggttgatat taacgatgaa ctggtccgca cagtaattac
       61 cgttgatgac ggatgcgacc atacccagcg ttttatctgg atgatgaata cacggcgtaa
      121 tatcagaaac aaagtgttcc ggcacgaacc aaagccagtc agggtgacaa agatacgcat
      181 tgaagccaga aaacgccgca aatacaggcc acggaaggcc tgggccccag cgcaaccgga
      241 tgaactggca taaagacagc aggagaataa gtcgtgaatc gcgagtttga aatatggata
      301 cgactcagat acgggtaccg atacgattta accagagatg ctcacggctt ctactgccgt
      361 gaagttgtaa agcgaatgtt tgacgtgtgg tgccactgcc gtggatgaaa attttatgag
      421 gttggcatgc agacaatcat ctatcagata acccccagca aatggtgtac ggagagagtc
      481 ctcattgcat caacagggct aaagcctggc accattgagc gggcaagaag aaagtcatgg
      541 acgcagggaa aagaataccg ccattacgct gtagaaggtg atccagggca ttacagtgaa
      601 tgcctgtaca acatcgaaga aattatgcga tggatcgaaa accagaaaca accaggtgtc
      661 aaaaatgcaa gttccggtta acctgttaat gctcctggac gtctgggagg ttcaatgagt
      721 aacacatcat acccgacagg cgttgaaaac catggcggat cactccgcat atggtttcac
      781 tataacggca aacgtgtcag agaaaacctc ggtgttcctg acacagccaa aaaccggaag
      841 attgcaggcg aactcaggac ttcagtttgt tttgcaatca gaatggggaa tttcgactac
      901 gctgcgcagt ttcctgagtc cccaaatctg aaacactttg gtctgggaaa aaaggagata
      961 accattaagg cgctttcaga aaaatggctg gatctgaaaa aaatcgaaat aggaggtaac
     1021 gcattcaatc ggtatcaatc tgtggtgaga aacatgttgc ctcgtatagg ggaaagccga
     1081 ctggcctcat ctgtaacaaa agaagattta ctcttcatca ggaaagattt attgaccggg
     1141 tatcacaatc tctcaaacgg caaaacaaca cctgttaagg ggagatctgt agttacagtt
     1201 aattactaca tgacgacaat tgcaggtatg tttcaatttg cagctgacaa cggctacacg
     1261 gaatcaaacc cgtttaacgg cctgacacca ttaaaaaggt ccagaaccga gccagatccg
     1321 ctcacgcatg acgaattcat tcgttttatt gatgcttgcc accatcaaca aacgaaaaac
     1381 ctgtggtctt tagcagtata cacaggcatc cgtcacggtg agctaatatc tctcgcctgg
     1441 gaggatattg atttaaaagc taaaacaatg actatccgtc gtaattatac aaaactcggg
     1501 gagtttactc taccaaaaac cgaagcggga acagatcgtg ttattcatct tgttcaacca
     1561 gccattgaaa ccctgaaaag tcaggctgaa atgacaagat ttggcactca ataccagatt
     1621 gacgtcaaac tgcgggagtt cggacgcacc gtaagccatg aatgcacgtt tgtctttaat
     1681 ccgcaactgg tgaaaaagtg ccagcaagcc ggttaccact acaaagcgga ctccatcagg
     1741 gattcatggg catcagccct gagacgggcc ggactacggc acaggaaagc ctatcagtca
     1801 aggcatactt atgcctgctg ggcactggcc gcaggagcga atccaagctt tatcgcaaac
     1861 cagatggggc acgcaaacgc tcagatggta ttcaatgttt acggagcatg gatgaaagat
     1921 aacaatatcg ggcagataga actgctcaat aaggagttag ctgaaagtgt cccatacatg
     1981 ccccacagag caagacgctg aaataatttc tttcagtatc attgagttaa cccatggttc
     2041 ccgccatgtt aactgcgtgg agggtaacac cacgctttat gccctgccga aacccgaggt
     2101 tgtcctgcgc tggcgtgagc agaccacaga tgacttccgc ttctgtttta agtttccggc
     2161 gaccatttcg catcaggcag cattacggca ttgcgatgat ttagtgactg aatttttgac
     2221 ccgcatgtca ccgttggctc cgcgcattgg acaatactgg ctgcaactgc ctgccacatt
     2281 cggcccacga gagctgcctg cgctttggca ttttctcgat tctcttcccg gtgaatttaa
     2341 ttatggggtg gaagtccgcc atccacagtt tttcgccaaa ggggaagagg aacaaacgct
     2401 taatcgcggt ttacatcagc gcggcgttaa tcgggtgatt ttagacagcc gcccggttca
     2461 tgcagcacgt ccacacagtg aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc
     2521 ggtacatgct gtactgacgg cgaaaaatcc actgatccgt tttatcggta gtgatgatat
     2581 gacgcaaaac cgggagttat ttcaggtctg gttacaaaaa ttagcacagt ggcatcagac
     2641 cactacgcct tatctttttt tacatacgcc agatattgcc caggccccgg aactggtaca
     2701 taccctgtgg gaagacttac gtaaaacgct tccagagatc ggagcagttc cggctattcc
     2761 acagcaatct tctcttttct gaatttgcca cctatcatag acaggtgcca tcggccattt
     2821 taaagggagt ttgtatggta agcgcgctgt atgccgtttt aagtgcgttg ttattaatga
     2881 agttctcttt tgatgtcgtt cgcctgcgaa tgcagtaccg cgttgcctat ggcgacggcg
     2941 gttttagcga actgcaaagc gctattcgca ttcatggtaa cgcggtggaa tatattccta
     3001 tcgcgattgt gttgatgctg tttatggaaa tgaatggcgc agaaacctgg atggtgcata
     3061 tttgcggcat cgttttgctt gctggtcgtc tgatgcatta ttacggtttt catcaccgtc
     3121 tgttccgctg gcgacgttct ggcatgagcg cgacctggtg tgcgctgttg ctgatggtgc
     3181 tggcgaatct ttggtatatg ccctgggagt tggttttctc cctgcgttag cgcacaatac
     3241 gccactttct ttttcccgga tttttacgtt atgtctcacc gcgacacgct attttctgcc
     3301 cctatcgcca gactgggcga ctggaccttt gatgaacggg tagctgaagt cttcccggat
     3361 atgatccagc gttccgttcc cggctattcc aatattattt ccatgattgg tatgttagcc
     3421 gagcgcttcg ttcaacctgg tacgcaggtt tacgatctgg gttgttctct gggcgcggcg
     3481 acgctctcgg tgcgtcgcaa cattcatcat gataattgca aaattattgc catcgataac
     3541 tcccccgcga tgattgaacg ctgccgtcgt catattgacg cctataaagc ccctacgcca
     3601 gtagacgtca ttgaaggtga tattcgcgat atcgccattg aaaacgcatc gatggtggtg
     3661 ctgaatttta ccctgcaatt cctggaacct tccgagcgcc aggcgttact ggataaaatt
     3721 tatcaagggc tgaacccggg cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat
     3781 gccaaagttg gtgaactgtt gttcaacatg caccacgact ttaaacgtgc caacggttac
     3841 agcgaactgg agatcagcca gaagcgcagc atgctggaaa acgtgatgct gaccgattcc
     3901 gtggaaaccc ataaagcacg cctgcataaa gctggttttg aacatagtga gctgtggttc
     3961 cagtgcttta actttggttc actggtggca ttaaaagcag aggacgctgc atgatcgact
     4021 ttggtaactt ttattctctg attgccaaaa atcatctttc acactggctg gaaacgctgc
     4081 ccgcgcagat tgctaactgg cagcgcgagc agcagcacgg gctgtttaag cagtggtcca
     4141 acgcggtgga atttctgcct gaaattaaac cgtatcgtct ggatttattg catagcgtaa
     4201 ccgccgaaag cgaagagcca ctgagcgccg ggcaaattaa acgcattgaa acgctgatgc
     4261 gcaacctgat gccgtggcgc aaagggccgt tctcactgta tggcgtcaac atcgataccg
     4321 aatggcgttc cgactggaaa tgggatcgcg ttctgcccca tctttctgat ttaaccgggc
     4381 gcaccattct tgatgtcggc tgtggcagcg gttatcacat gtggcgcatg attggcgcag
     4441 gcgcgcacct cgcggtggga atcgacccta cgcaactgtt tctgtgccag tttgaagcgg
     4501 tgcgtaaact actgggtaac gatcagcgcg cacatttgtt accgttaggt attgaacagc
     4561 ttccggcatt gaaagccttt gataccgtct tttcgatggg cgtgctttat catcgtcgtt
     4621 caccgctgga gcatctctgg cagttaaaag accaactggt gaatgaaggt gaactggtgc
     4681 tggaaacgct ggttattgat ggtgatgaga acacggtgct ggtgccgggc gatcgttacg
     4741 cgcaaatgcg taatgtctat ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga
     4801 agtgtggttt tgtagatatc cgcattgcgg atgtgagcgt taccaccaca gaagagcagc
     4861 gacgcaccga atggatggtc accgagtctc tggccgattt tctcgacccg catgatccgg
     4921 gtaaaacggt ggaaggttat cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt
     4981 aaaggtctgg tattactgcc ggatgcggcg tgaacgcctt atccggccta caaaaccttg
     5041 ctaattcaat atattgcagg gactatgtag gccttataag catagcgcat caggcagttt
     5101 tacgtttgca taacctcagc gcccgtttcc gggcgctttt cacgtcttat gcctgaacag
     5161 ctggctgatc aaacgccgtt aacttcggtg cattgcccgt atatttttcg atatacacca
     5221 gcgcagagtt accggcacag gcatttgcca gctgagagct ggggatatcc gccgttaaca
     5281 cgttcgcact gccgttttta cacaagccat tttccagatc tggccatgca ccttcatgca
     5341 cgcataccac gccttttttg atcccgtcag tcaccaccgc gcctgtcaga atctgaccac
     5401 gtttgttcca gacacgcacc agatcgccat tcgcaatacc aaagcgagca gcatcttcgg
     5461 tgtgaatagt gatcggttca cgatcggcaa ccgcatattt tttacgcagt tccgcatagt
     5521 taagctgact gtgtaaacgg tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt
     5581 cggcggaacc tttccactca tcaggcgcaa gccaggttgg gtgtgccggg caatccttat
     5641 agccaaattt ttccagcgtt ttggagtaaa tctcaatttt gccgcttggc gtacccagcg
     5701 catttttcac cggatcggcg cggaaatcac cataacgaac gtattgttcg ttcttctcac
     5761 tgcggcgcat ttcgatcagt ttattttgct gccagaaagc attaaacatc ggcatagtca
     5821 cgcgttgcgc acgggcacct ttctgagcgg catcatagaa aaatttcagc cacgccattt
     5881 catctttacc ttcggtatag atctcttttc cgccaggttt gagtaattca gcaagatcgg
     5941 caaacacgtc aaaatcgttg cgcgcttcaa attgcggagc gacagcctgc ttcatcggca
     6001 caatatgctg gttgctgtaa tcaccggtca tcgtcaggtc attgcgctca aacgatgtgg
     6061 tgatcggtaa tacgatatct gcatgtttag cagctgcggt ccagtagcat tcagaaacga
     6121 cgatcatctc cggtttctgc catgctttaa tcagacgatt agtatcctgg tgatgggtaa
     6181 agttaccgcc acccgcccac cagatcattt tgatattcgg atacgtttgt tctttaccgt
     6241 tatgctgata tttgccgcca ggattttcca gcgcatcgac gatacgggca acaggaatcg
     6301 ccgtcattcc gccatcatca gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt
     6361 ccggcagcac gccgccaaca cgcgtcgggt tgccaccgtt ggagtaatga taagagaatc
     6421 caaaaccgcc gcccggtgta ccaatttgcc ctaacatcgc cgccagcgtc accagcatcc
     6481 agtgtttctg ttcaccgtat tgctggcgtt ggatccccca gcctgccatc agcatcgtgc
     6541 gattcgccgc catcagttct gcaagtttga caatctgcgc ttccggtacg ccagtaattt
     6601 ccgccgccca ggccgcgctc ttcggcgtat tatcgctctt acctgtcaga tactcttcaa
     6661 actgcggata cccggtagtg tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca
     6721 tcagggtatg cgcaatccct aacatcagtg ccacgtcggt gcccatattc ggtgcgatcc
     6781 aggtggcatt atcgccaaag aattcgatgg tttcggagca gataggatca atggcaatca
     6841 ctggtttgcc agattttttc agctgatgga agtattccag cccttgctca tcggtactgc
     6901 tccaggcaat tttgagcgtg ttcagcgggt tcatccccca cagcaccaca acctggctgt
     6961 tttccagaat cagcggccag gaggtctgct gttcatacac ctctacagaa ccgaccacat
     7021 gcggcatgat cacctgtgcc gctccggttg aataatcgcc gctatgcccg gaataaccgc
     7081 ccgccaggtt catataacgt tgcagtaagg tttgcgcttt atgcaacacg ccagaagaac
     7141 gccagccgta agatccggca aaaatggccg atggtccgtt agctttacga atacgatcgt
     7201 gttgctcatg aatcagtttt aatgcttgtt cccagctcac ctgtacatag gtatcttcgc
     7261 cacgaccttt cgccggttgc agtggattat cgagatagct ttttctcacc atcggatgct
     7321 gaatacgcgc cgtggtgtgt acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg
     7381 ttttcgccag cgcgcctgtc gaagaaacaa tcttgccgtc cttcacttct acgttcatcg
     7441 ctccccaacg gcccgcggtg agaattttgc cgcccttctc ttctgcccat gcaggtaacg
     7501 gtgcagccga tgtcaccacc agcgcccctg ccgcaatacc gctatgttta ataaattcac
     7561 gtcttgttaa tgtcataact tcctccctga tcaacgagga tcactgtttc tcggtaatat
     7621 ctttggcgtt gtactggaag taccgcgtca aaatgtccag ttcgttttcg ctcatgctgg
     7681 ttcgcgcacc cattcctttg gcaatggacg gccatgcatt gacggtgtaa tggtcggagg
     7741 caataggggc atggcaacca gcgcaatagg tatcgtcaag tttttcagcg tattgccata
     7801 gaggtttacg gtccgctaat gcgggatcgg taagcacacc ctgtaaagac gcctgacgcc
     7861 attgattgcc gtattcgtca gcctgccatt ccccgtttac cgtcagcgcc ttgatacctt
     7921 cttcacttaa tgtggctagc gccagccgtt gacctgccgc caggtagagc gtgttttcac
     7981 tgccctgcat ttgataaccc tgcaacagaa cgatcggctg tttgccactg gcatcaacga
     8041 cggtgagatc ggtaccagga ttcacggtag ccagttcgcc tatgcgagaa gttttgaaag
     8101 gataaatatg tgcgccatta gtaactgaag tagcggcctg actttccagc tcatgcgccg
     8161 cgttgtcatc catttttatt tctggcggaa aatgggcaat gcctttatgg caatcgatac
     8221 aggtttcgcc gtccttttgc gctttattat gcattttctg cgcagattca ctttgcgagg
     8281 caatatccat ggcatcaaaa gaatggcaac tacggcaggt tgcagagtca gtggctttta
     8341 attctttcca tactgtttcg gccatttcct ggcgatgagc ttcgaactta tcatcactgt
     8401 ctattttgcc gctaacaaat tcatgataaa tatctttaga tgcttttaat ttagcaaata
     8461 aataatccat ccctgacttt ggaatatggc aatcggcaca ttccgcacgt atccctttct
     8521 ggtttgaaaa gtggacagtt ccctgatatt cctcaaaagg tttactcatc gagtggcagg
     8581 aaaggcaaaa tgcggtatcc gacgttttat gtaaggcttt ttgcgccagt aataacccgc
     8641 cgccaccaac cacaaccgct atcagcaaaa acaataaccc aatgcgtttt ttccctcgca
     8701 tagattcctc taattaaatt ccaggacacc gataccattg tataaataaa tacacaacga
     8761 attgactact tgcttaattc ggcacaacac tgcctgaaat aatcgataaa gaatatgatt
     8821 tattacaatg taatcattaa ttgctaagga ataacctagt tgttatttag aatatatatt
     8881 tgtcagtttt ggcaatttaa atcacataat gaatgtgaat atataaaata tttttagcac
     8941 aacgctatgc gcactgtcac ccgctcaaca gatcacggca atcgttcgtt ttttatactg
     9001 ctcaga
//
LOCUS       DAZGHF010000173         8879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGHF010000173 DAZGHF010000000
VERSION     DAZGHF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.174"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..777
                     /locus_tag="ACHK3A_004409"
     CDS             <1..777
                     /locus_tag="ACHK3A_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO8742409.1"
                     /translation="FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAP
                     FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQI
                     GTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICT
                     GRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCR
                     RGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1145..1969
                     /gene="gadX"
                     /locus_tag="ACHK3A_004410"
     CDS             1145..1969
                     /gene="gadX"
                     /locus_tag="ACHK3A_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO8742410.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
     gene            2338..3066
                     /gene="gadW"
                     /locus_tag="ACHK3A_004411"
     CDS             2338..3066
                     /gene="gadW"
                     /locus_tag="ACHK3A_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO8742411.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLAADFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(3429..6542)
                     /gene="mdtF"
                     /locus_tag="ACHK3A_004412"
     CDS             complement(3429..6542)
                     /gene="mdtF"
                     /locus_tag="ACHK3A_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO8742412.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGDHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6567..7676)
                     /gene="mdtE"
                     /locus_tag="ACHK3A_004413"
     CDS             complement(6567..7676)
                     /gene="mdtE"
                     /locus_tag="ACHK3A_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO8742413.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7784..8062
                     /locus_tag="ACHK3A_004414"
     CDS             7784..8062
                     /locus_tag="ACHK3A_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742414.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYIYQGNKVVFASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8063..8590)
                     /gene="gadE"
                     /locus_tag="ACHK3A_004415"
     CDS             complement(8063..8590)
                     /gene="gadE"
                     /locus_tag="ACHK3A_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO8742415.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNNPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         2261 a         2108 c         2117 g         2393 t
ORIGIN      
        1 ttcggcgtga cctacaccgg taactatgag ttcccgcagc cgctgcacga tgcgctggat
       61 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagcggtggc
      121 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      181 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
      241 tggcgtgatg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggcggt
      301 cagattggta ctttcgccat caacttctcc cgcccggcgg gtcaggtgat tgcacagtac
      361 tatgaattcc tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ttcctaccag
      421 gttgccgctt atctggcgga tgaaatcgcc aaactggggc cgtatgagtt catctgtacc
      481 ggtcgcccgg acgaaggcat cccggcggtt tgcttcaaac tgaaagatgg tgaagatcca
      541 ggatacaccc tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc
      601 ttcactctcg gcggtgaagc caccgacatc gtggtgatgc gcattatgtg ccgtcgcggc
      661 ttcgaaatgg actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc
      721 agcgaccacc cgaaactgca gggtattgcc cagcagaaca gctttaaaca cacctgataa
      781 catcacgttg taaaaaccga atgcccaacc tttaaaaaca ggtgggtatt cggctactta
      841 ttaattgcaa taatatatta cctgtctatt catcgtgttg ataagatata tgttatatga
      901 atgttatgga gcatagcgat actgcttaat attacgatga tgaattattt gcttactatt
      961 aattcctctg cgttcttaag tccccttttt atataataaa tccagtcatc cagcccgagt
     1021 atcatcgctc attatcatcc tgttttcccg cttcgtttaa atttatttat caatcaattt
     1081 gacttaagag ggcggcgtgc tacattaata aacagtaata tgtttatgta atattaagtc
     1141 aactatgcaa ccactacatg gtaattgtct aattgcgtac gcaagacata aatatattct
     1201 caccatggtt aatggtgaat atcgctattt taatggcggt gatctggttt ttgcggatgc
     1261 aagccaaatt caagtggata agtgtgttga gaatttcgta ttagtttcaa gagacacgct
     1321 ttcattattt ctcccgatgc tcaaggagga ggcattaaaa cttcatgtac ataaaaaagt
     1381 tccttcatta ctcgttcatc actgtaccag agatattcct gtttttcagg aagttgcgca
     1441 actatcgcag aataagaatc ttcgctatgc agaaatgcta cgtaaaaggg cattaatctt
     1501 tgcgttgtta tctgtttttc ttgaggatac gcagtttata ccgttacttc tgaacgtgtt
     1561 gcaaccgaat atgcgaacac gagtttgtac ggtcatcaat aataatatcg cccatgagtg
     1621 gacattagcc cgaattgcta gcgaattgtt gatgagtcca agcctgttaa agaaaaaatt
     1681 gcgcgaagaa ggtacgtcat attcacagtt gcttactgag tgtaggatgc agcgtgcttt
     1741 acaacttatt gttatatatg gggtttcaat taagcgagtt gcagtatcct gtggatatca
     1801 cagcgtgtcg tatttcattt acgtttttcg aaattattat gggatgacgc ccacagagta
     1861 tcaggagcga tcggcgcagg aattaccgaa ctgtggctcg gcggcaagta tggctgcgca
     1921 agggattttt tacggcactg accgttctgc ggaaggaata agattataga gttttactca
     1981 gacataaaaa aaacccggca taggggaccg ggaagaggat agtctgccgt ctccagacta
     2041 ataaaccgtt ataacactcc ctgttggcac gggaaacttt gtgctctcag taagttaaat
     2101 ataactttta ctggaaataa gatcagccat ttttttataa acataagcta tacgctgtgt
     2161 gaaaatataa tcgtgttgca tttacttatt atcaattaac tgttatgcaa aactactttg
     2221 tggataaaat ttggtcctac caaatatggc agtttttgca cacagaaaca gtctggcgtc
     2281 atttcattag tatactgaca ttgaaataat cgcagtaatg aaatataagg gatagtcatg
     2341 gctcatgtct gctcggtgat cctcgttcgt cgttcattcg atattcatca tgaacagcaa
     2401 aaaatatcgt tgcataacga gagtatcctg ctgctggata aaaatttggc agccgatttt
     2461 gcgttttgtt cactggatac gcgacggctg gatatcgaag agctgacagt ttgccattac
     2521 ttacaaaata ttcgtcagtt gccacgcaat ttaggattgc atagcaaaga ccgtttgtta
     2581 attaaccagt caccccccat acagctggtg acggcgattt ttgatagttt caatgacccc
     2641 cgggtcaatt cgccgatact gagcaaaatg ctctatcttt cctgtttatc aatgttttct
     2701 cataagaaag aactgatccc cttacttttc aatagtatca gtactgtttc aggaaaagtt
     2761 gaacgcctta ttagctttga tatcgctaaa cgttggtatc tacgcgatat cgcagaaaga
     2821 atgtacacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac ctgtttcagt
     2881 aaaatattac tcgcctccag gatgtcgatg gccagacgat tactcgagtt acgtcaaata
     2941 cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcatactt tataaataca
     3001 tttagacaat attatggtgt aacgccacat cagttttcgc aacattcgcc gggtaccttt
     3061 tcctgacata tttcgcattt gaatattggt caggatctca cgtctgcttc atgtgaaact
     3121 ctttcctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt gccaggcggt
     3181 ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct aacaattcat
     3241 acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa tggctggagt
     3301 ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag ctggttgttg
     3361 agtggtgaat ggttagcagg aaagagtaag gctgaaccct cacgttcaac cttactctca
     3421 tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac gaaaaagacc
     3481 ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccattac gccggtacct
     3541 accgcgtttt gcgcgccaga gccggcacca tgactgataa ccagcggcag cacgccgaga
     3601 ataaaggcca gagaagtcat caggattggg cgtaaacgca tacgcgctgc ttcgatgatt
     3661 gcctctaccg gcgttttccc ttctttctgc atcatctcaa cggcaaattc gacaatcagg
     3721 atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa gtaaacgtca
     3781 ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa cggaacaacc
     3841 aacatcaccg agaacggaat tgaccagctt tcataaagtg cggcgagggc gaggaacacc
     3901 acgaccagtg aaatggcata tagcgcggga gcctggtttg aggataacgc ttcctgatac
     3961 gacagcccgg tccatgagta gccgacgcct gccggaagtt ttgcgaccag gtctgccata
     4021 aatttcatgg cgtcaccggt acttttcccg gcagccgctt cacctaaaat ctccattgac
     4081 gggatgccgt tgtagcgttc cagtcgcggt gaaccatagg tccattcggt agacgaatag
     4141 gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt gatgttatcc
     4201 ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg cccctggttg
     4261 aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt gatatcagac
     4321 agcgccacac ccatagcctc ggctttcgca gcgttgacgt tcactttgaa catcggcgta
     4381 tcttccaggc cgttcgggcg taccccgatg acctgattcg gtgattgcgc tgccagtgat
     4441 aacagctcgt ttcgcgcctg ggtcagcttt tcgtgcccca gattgccgtt gtccagcagt
     4501 tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt gaacgggaag
     4561 acgacggctt tattgatact gcttagcgca atcattgccc gctgaataat cgcggtaacc
     4621 gagttttcct cgccgacacg ttcagaccac ggcttgaggc tgataaacgc cagaccgttg
     4681 ttttgcccct gaccactgaa gccaaagcca ccaacggtaa acaccgactg aacattattt
     4741 ttctcttttg tcagataata atccgtcacc tgttgtagca ctttcgtggt gttaaccatg
     4801 gttgcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc ttctggtaag
     4861 aaagaggtcg gcgtacgcag gaacagcacc gccatcccgg cgcaaatcag caggtagacc
     4921 accatgtagc gaccggtaca acgcaacagc gagcgggtgc tgtcggtata gtgctgagtt
     4981 gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg atcgccctcc
     5041 ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc tacaaatact
     5101 gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc cccggtcgca
     5161 ccgctcataa aggccatcgg cataaacact gcggaaagaa caacggcaat cccgaccagc
     5221 gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag cttatcttcc
     5281 gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag taaccctatc
     5341 gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc cgacaagatc
     5401 gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt ggcgcggaaa
     5461 ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac cagcgttttg
     5521 aaaacttcct gaatagaaat tttgataaac ggtgtggtgt cgtaaggata aaccgtcttc
     5581 aggcttgcag ggaaataagc cgataaacgg ttcagctcct ctttcaccgc tcgcgaggta
     5641 tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg tttgccgtta
     5701 tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac atcacgcagc
     5761 agtacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc ttccggcgtt
     5821 tgcagacgcg tctgcacaat gatcgaggcg ttcagttgct ggtctgccgc ctgtggcatg
     5881 ccacccagtt gaccaccgga aatctggtta ttttgcacct taatctggga aataacatcg
     5941 gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg catggcgtac
     6001 tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg gtctttgata
     6061 ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc agaaataaat
     6121 gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg ctgctgcacc
     6181 gcttcaggta atgaaggcat agcgagctgc agtttatttt gcacctgaac ctgtgcgata
     6241 tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc cgcatcactg
     6301 gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat cacctgagtg
     6361 accgagtctt ctaccgtttg cgcatcggca cccggatagg ttgcgctgac ggtaatggtc
     6421 ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag accacctgca
     6481 agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa atagttagcc
     6541 atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc gttttcctgg
     6601 ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc ggaaacgatc
     6661 acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat ggctttgctg
     6721 gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc cgttgcttta
     6781 ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg gcggctacct
     6841 tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg gttggggaaa
     6901 atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga gaatttcagc
     6961 gtgccggtct ggctgtaacg tttaccattt tccagattga gctgtactgg cgtactgccc
     7021 tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa atcctgcacc
     7081 gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag cgaatctgcc
     7141 tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac gcccgtaatc
     7201 ggcgaggtga catttgcgta ttgcaggttg atagtcgcct gttcaacagc cgctttggcg
     7261 acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtgtcgta atcctgacgg
     7321 ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg ggcattgctg
     7381 gcggtagaga gcgctttcgc cagcgagcct ttggcggagt tcagctctgc ctgtaaaggc
     7441 gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc gataaagttg
     7501 cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata aggaacggtt
     7561 ctaccaggca attcgctcaa cacattgacc gaaccggggg agagtgtgac gacaccgacc
     7621 tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt gagcatcgcg
     7681 ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt ccctgaaaat
     7741 tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg atttacagac
     7801 cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg atcgacatgg
     7861 tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca gcattcgggc
     7921 aacgaagggt tctactggtg gatacatata ccaggggaat aaagtcgttt tcgcctctcc
     7981 tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg ccagccatca
     8041 atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg acgatgtcgc
     8101 tcatacgtct tagtttcagt ttgtacataa tgttgaattt ctgaatcttt aatgtttgtt
     8161 gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga atcagttgca
     8221 taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc aagtgtttac
     8281 cataagtaat ctcaagaaga tttttgatac tttctttgcg gctgacaaaa taaataggcg
     8341 ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc tcggcatcta
     8401 atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag gtatcaatga
     8461 taaccttgaa ggggttattg cctacttttt tgatctctga cagggaattg atttttatca
     8521 tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct ttcgtcatga
     8581 gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc ttttcattat
     8641 ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac tttgcccttc
     8701 gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc acgcactcca
     8761 tatattggtc ctaagtaaga ttcctggttg ttatcagttt gtaactagta aacaaaaggt
     8821 taacttttgc ggattactgc ccaagaataa gttatcaata accagtttgt gatctctga
//
LOCUS       DAZGHF010000174         8427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGHF010000174 DAZGHF010000000
VERSION     DAZGHF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.175"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..350)
                     /locus_tag="ACHK3A_004416"
     CDS             complement(<1..350)
                     /locus_tag="ACHK3A_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742416.1"
                     /translation="MIRIIVFLLFFLSGCTSQKEYSNIVRIENRCSSDIDVFIQGYVS
                     GSTLIYQHNIHLPVGDSDVLVYFSTADIDDGFKYEGAFMIYKDSRGELDYPFTISLSG
                     REKKFGKNDLIRLSR"
     gene            complement(343..1749)
                     /locus_tag="ACHK3A_004417"
     CDS             complement(343..1749)
                     /locus_tag="ACHK3A_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO8742417.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEALSLEVLDGNEVIYSERQTGKYCDKGEHAWQWDGYSNQGILDTTKIKS
                     KSLLVRLIALCGDMMIKVDYPLKNAAKEEEWVDVKVDRNNKVVDITWRLSVKDSGTKG
                     SNPELSPIPYSDLVSLTKNGVEFYWSRNGSRGGGIGGNIVTAIGVFKVNVKAEINITP
                     SMRTFDLISSLDPDMQSSVSLLGFEKIYHNYGGYYNNSKDEIKALLYSNERYKWDSAH
                     EMGHKVMDKYGSESWTHKGTSTRGQSTIPNTPMPSSGEIDIMKYADSYAPDRYKRLVA
                     ADEDVQALIWLSRVSFND"
     gene            complement(1755..3365)
                     /gene="tssA"
                     /locus_tag="ACHK3A_004418"
     CDS             complement(1755..3365)
                     /gene="tssA"
                     /locus_tag="ACHK3A_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO8742418.1"
                     /translation="MSELLQKLTRSCFADRDALDSARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGAQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLTVSFTAWPEDQHSPSLMPLINALESRLAQSGRMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADELTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDIAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            complement(3358..6684)
                     /locus_tag="ACHK3A_004419"
     CDS             complement(3358..6684)
                     /locus_tag="ACHK3A_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO8742419.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQSFELHRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSTEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQT
                     GRPLQSVGCLLSERCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GIAHWQSILKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSPVWAGVTGDNAR
                     GRTVGFPWLRITLMSAVCVLAIWGAGMTTSFFANRALVQETSIQTARALDIRLPLSEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADDLRY
                     AGVPEGVRQSVLPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDMLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGSLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWDQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            complement(6741..7955)
                     /locus_tag="ACHK3A_004420"
     CDS             complement(6741..7955)
                     /locus_tag="ACHK3A_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742420.1"
                     /translation="MPIRLNTIPDAEPYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVGSVRWLVFVTQHIRADAWNQRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARHQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGMWTVELNREQRASLLSS
                     NQPFTQEALMYELDAFLGCSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQNGHYSWAT
                     MVSPFVSPQEAS"
     gene            complement(8061..>8427)
                     /locus_tag="ACHK3A_004421"
     CDS             complement(8061..>8427)
                     /locus_tag="ACHK3A_004421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742421.1"
                     /translation="SNSYSEEKIEIININKPIYIGTNQSDKVLITACNHWVLNSRDII
                     DIFNLSIKHDDYGEINRMYYWLPCEIRGRLMYDGIEWSFVINAAATAMWMSNDKEIYW
                     GCNKKACDNFFLLPYDGMR"
BASE COUNT         1817 a         2442 c         2131 g         2037 t
ORIGIN      
        1 cgtgataatc taattaagtc attcttccca aatttttttt cgcgccctga caatgaaatg
       61 gtgaatggat aatccaattc accgcggcta tctttatata tcatgaatgc cccctcatat
      121 ttaaaaccat catcaatatc tgccgtggaa aaatatacta atacatcact atcaccaact
      181 ggcaagtgta tattatgctg ataaatcagc gtagaaccgg aaacatatcc ctgaataaag
      241 acatcgatat ctgaggagca tctattctca atcctgacta tattggaata ctctttttgt
      301 gaagtacacc cactcaaaaa aaacaataaa aaaacaatta ttctaatcat taaaactcac
      361 cctgctcaac cagatcaatg cctgaacatc ttcatctgcc gcgaccaatc tcttatatct
      421 atcaggtgca tagctatctg catatttcat tatatcaatc tctcctgaag atggcattgg
      481 agtattaggg atggtacttt gaccccgcgt ggatgtcccc ttatgtgtcc atgattcaga
      541 accatattta tccataactt tatgtcccat ctcatgagca ctatcccatt tatatctttc
      601 attactatat agaagagcct ttatttcatc cttgctgtta ttataatagc caccatagtt
      661 atggtatatt ttttcaaacc ccaaaagact taccgacgac tgcatatctg gatctaacga
      721 tgatatcaaa tcaaaagttc gcatagatgg agtaatattt atctcagcct ttacatttac
      781 tttaaaaacc ccaatcgctg taacgatatt accaccaatt cccccgccgc gacttccatt
      841 tcgtgaccag taaaactcca cgccattttt agtaagacta actaaatcgc tataaggtat
      901 tggcgataac tcagggttac tgccttttgt accgctatcc ttaacagata atctccatgt
      961 tatatcgacc actttattat ttctgtcaac ttttacatca acccactctt cttctttcgc
     1021 agcatttttc aatggataat caacttttat catcatatca ccacataaag caatcagtct
     1081 tacgagtagt gatttgcttt ttatcttagt tgtatccagt atgccttgat tactgtagcc
     1141 atcccattgc caggcatgct ctcccttatc acaatattta ccggtctgcc gttcactata
     1201 aatcacttca ttaccatcaa gcacttccag agaaagcgct tcagcctcat cgcctttatt
     1261 tgtgatggta aaatctattt tttctgtatt cttcgtacta ccaccgctaa tcatttcttt
     1321 ataatcaggt atattcagtg gcgtcagtat cttattacct gataatttaa ttctgtaatc
     1381 cagttttaca tcatcagagg atgaatcaga accagaagtc agatcctttt tcgccgactg
     1441 cgcatgttgt tccgcttcac tttccccgtc atccgtctca tacgtatcat ccatcattga
     1501 cgcaataaac cgtgccttac acgggcaact gctctgactg tccagcgtcc ccgccatcag
     1561 cctgccgtga atatctgttt caggaacgcc gcccacaata ataaacattc ccggatatct
     1621 gccgcaggtg acccggtcca tatcccgcgc ctgcgggata cccatgatcg tatggtcgtc
     1681 cgcgccttca ataattttgc cgccgcaggt ggttttgtcc cccctgacca gaaagtagcc
     1741 tgttcccatt gccatcagtc cttatgttgc gtatcacaca ataccgctgc ccgtaccgga
     1801 tctattgtca ccagcgttcc cagcaggatt tccatttttc ggttcagcag ggctttatcc
     1861 gcatagcggt gcgcccgcag gcgcagcagt ttcagctgtc gggcttttac ctcaaacagc
     1921 aggtccggct cccactgcgt cagcgtcagc ccctgtgcgg cgatatccag ttcttccagc
     1981 agcaggagcg ccatctcgtt tttaccgtac tgttccgcca cccgcgccat cagcagtcgc
     2041 aataacaggc gctggcgtgc cgtggtgatg cccggacggg tctgtatcca gcccagcgcc
     2101 gcttccacgc cctgactgtc tgccatctcc agcgcttccg gctccagcgc cagtacgtcg
     2161 tttgtggcgt cgtctgtcgg tacagcggct ttccccgcaa gccatgcgcc gtcttcccgc
     2221 atcatgacct gctgcgctat ccagttgcgc gtcaactcat cggcgaacgg cgtgccgtcg
     2281 ttccatgcca gattttccag ccccggcagg cgttccagta gtaacgccag gtcctgacgg
     2341 ataaccgctg tccacctgtc ccacggtgcg cccgccttag tcagcgcctg atgcagatac
     2401 cactgaatgt ccagccagaa atggctgacg ccggtgctga acatcaggtc ggcctgctcc
     2461 agcagttccg tccagttctg ctgagcatac agccgcttaa gctggttgcg tgattctgtg
     2521 cgcggcgggg ccagccgggt ttttccgtcc acatccggcg gcagttcatg caccgtgtcc
     2581 cagcgcaggg ttttcatcag ccggtgtgct gaaagccagc cctgcggctg ttcgttcaga
     2641 taacgggcca gcactttcgc ctggtcgagc aggtcgcgcc cggaagtgat ggtctgtacc
     2701 tgtggcgcat ccaccgggga agacggtgcc ggaacgccgc tgctgttctg cggcaccacc
     2761 gcgttcatcc tacccgactg cgccagacgt gattccagcg cgttaatcag cggcatcagg
     2821 gagggggaat gctggtcttc gggccatgca gtgaaggaaa ccgtcagttg attaagagcg
     2881 gcaacaatgt tcgcaaaatc ctcttttgcc acttccgggt agcgggcaag gctgtcgagc
     2941 atcttttccc ctgccagcca ctccagcgcc atttttcggc ttgccgggcg cgagggcagc
     3001 agttgcgcac cgaagcgttc caccagtccg gctaacagcg ccagcccctc agccagtccg
     3061 cgttcgccat cgcggtgcag tcgcgcccag atatagtaag tggcgatgcg cacgtctttt
     3121 gcctgcgtca gcagcaggga ttcggcaagc tggcagataa ggtccgtatc cgcgccggaa
     3181 agtttgttca tctcgtcgcg gatacgcagg aaatcgtcgt ggtagccggg gtcttccccg
     3241 accggcgtat ctgccgtcac cggcagcagc catgtctgcc acagcgccgc ctgtgttcgc
     3301 gccgagtcca gtgcgtcccg gtcggcaaaa cagctacggg tcagcttttg caacagttca
     3361 ctcatcggcg tcttccccgt attcttccgc atccttcata ctcctgccgg tcaggaacac
     3421 ctgtcccggc aggcggaagt ttttcagttc cagcagcgcc agcggccctt taccctgttc
     3481 aacccgcagc aggtaattca gcggcaggcc gtcctgcgct ttccacacca ctttaaaggt
     3541 actgctgtcc accggtgtca cctgcgcctg ttccagcagg cgaataaggc tccagcttcc
     3601 ccgataatcc gccagtatgc gttccatcgc ctgcgtactg gtccagctca gcgatgcgcc
     3661 gggttgccac tgttcatccg gccagttaaa acgctgccag cgttccttct ggttgaaata
     3721 ttccagctcc tgcccgtcaa tcaccagatg cgtcttcatc acgtcgcgcg ccggttttgc
     3781 ccgcagttca aaatgtatcc ctgcatcgcc gctggcaaag gcggtatcgg ccacatcgcg
     3841 caggcggttc agggcgcgaa taaagtccgg attgactgtc agcccctgcg tgttcatggc
     3901 gtcagccacc cagtagcggc cttcacgttt cagtacgccg gaaagattgg cggcgataaa
     3961 ctggttgatg cgcccgtcat cgcgcaggta ctgcgccagc agggggaggg aggcatcgtt
     4021 ttgtgaggct ttgaacgggt agcgaccagc gaagtcctga tcccagtggc tgacaatcgc
     4081 ccgctgccac tggcggttca ggctgtctgc cgcaggcgtc agcacctgcc gccatgactg
     4141 ctccaccggg cggacgaaca gcgcctgacc gaagccactc cactcttcac ccagactggc
     4201 ggctatcagg ctgccatagt cgcgggtgtc ggtaaggtca acggttttgc cttgtaagac
     4261 cgtctgcgcc agttgctggg tcatctcctg cgggtcaggg gcgttggtca cctgttgcag
     4321 tttgaggcgc acgcgggtga ctcgggtgag gtaggtctgc aggctcagct ggctgtcgcc
     4381 gcctcctgcc ttatcgccaa gcaaccgcag cagcggcgcg aaggttttat ccagcggccc
     4441 gacaggcacg tcgccttcac gggattgctg cggcgttttc tccttgccgt taaacagttc
     4501 ctgtgccgat ttcgccagcg agtctgacag gccacggttt tccctgccag ccgccgcctg
     4561 ccacgcgagg gtgtctgtca gcgcaatcag cggtgactgg cgggcatcgg ccatcagcgt
     4621 caactggtcg agaatgccgg agagcgagtc ttcctttttc cagcgaatgc tgttgagaaa
     4681 cgccagccag cttccggcaa agtcggtaaa gtagcgtgaa gtgagacggt tacgcagcat
     4741 atccggcgag atatccgcag aggtatcctg ctggcggtcg ctgagtaccc agtcgatttc
     4801 ctcgcgccgc gccgtcacca cctgctcgat ggcttccctg acctgtcctt cccacgcctg
     4861 acgggtaaac atccccggca ccgtctgctc cgtactgaaa agagattcgg tgagcgtatc
     4921 cccggtcatg tccgccagcg tcatatcggc gtagttgcgg gacacctgtt gcagtacgtt
     4981 ctggtagaga gtgttttcgg cattgcgcac accaatctgg cgcagcagga ttttgcgcac
     5041 tgcgccggtc agttccggtg gcggcgaagt tttccactgt gggtgttccg gcagattcgc
     5101 catccagaag gtcagcagtg acggcaacac gctctgacgc acaccttccg gtacacctgc
     5161 atagcgcagg tcatctgcca tcagcgtggt actgaaaaag gcagcgtcgg ccttttccgg
     5221 gcgggaagtc atcagcaatg ccttaagctg cttatagcgc tgttcgccgg tggcggtacg
     5281 ctgcggacta ttgggcggca gcgcgacaaa ggcattcagt tgctgttgca gatggtcaac
     5341 ggccacgtcg cgcaccagcc ggtttgccgc ctgcgcatag ccgggaaagg cggcggcgag
     5401 cagttgctgg ttacgttcaa ggccaaaacg ctgataccac ggcgcaccct cccggatacg
     5461 gtactgcagg cgctccagtt caccctgcag ggtatgcagc gccaccagtt gttcagacag
     5521 cggcagacgg atatcgagtg cacgcgccgt ctggatgctg gtttcctgaa caagtgcgcg
     5581 gttggcgaag aacgaggtcg tcattccggc cccccatatc gccagtacgc agacagcaga
     5641 catcagcgtg atacgcagcc acggaaagcc caccgtgcga ccccgcgcgt tatcgccggt
     5701 cacgcccgcc cagaccggtg acggcagcca cgcatgaggt gcggcttccg gtacggcggt
     5761 aagcggcggg ctgaacatca ggccacgcag gcgcagggag gaaaatgcgc cgcctgccag
     5821 cagcggtttc aggatacttt gccagtgagc gataccccgc tctgcaaggg tatgggccag
     5881 acgcagcagg aaatcatggc ggttatcggt aagcagctgc gacatgccct gttccgtcag
     5941 cggcagggtt tgtgcttcca gcattgcagc cagttgttcc ggggtacagc gttccgacag
     6001 caggcagccg acgctttgca gggggcgtcc ggtctgataa tcaccgttgt cacagacctg
     6061 ccacaaatag agtggagcct gaaaaccgag tcgcttaccg ccgtttatca gttcgcgcca
     6121 gcctttgtcg agttgcgccg tctggcggct ctgttcttct gtcagcggcc agatgatgcc
     6181 gtccagcgga cggctgcggc gcagtttttt taaggcggtc agtagcgcga catcaggctc
     6241 tgtagatggc ctgccgccat atatcagcac cgtgtggtcg ccctccagcc agtgttgccc
     6301 ggtcagcccc ggcgcgatgg cctctgcctg ttcaggctcg ccggtcacca gcagcaggcg
     6361 gattttacgc cgccagaatg ggccgtagcg gtcgcgcagg aaggcggcga gttccaccat
     6421 tgccggatga cggctgtcgg ctttctttgt cgtcaccgcc cgatagtcat ctccggcggc
     6481 aagctgtctg tggagttcaa agctctgtac ggcagcgtgg cggttcagca gatgataaac
     6541 ccccatgata atcagaaaga ccacacctgc ggcaatcacg ccagccatcc agtacatttg
     6601 ctgtgaacta cccggttcaa cgcccagcaa tgccggttta cggtgaataa acgtgcaaag
     6661 cgcaaccaca acggccacgg tcatgacgat ggcaggccct gaaaccgaac ggggccggag
     6721 acggtttaat cccggaacca ctatgatgcc tcctgagggg aaacgaatgg cgacaccatg
     6781 gtcgcccatg aataatgccc gttctgaacg ccgctgagcg tcagttgggg gtgttgtgat
     6841 tgtattgccg ccagcgttgc cgcagcgacg gacagccacg gcgctgccgg tccggagcac
     6901 cccagaaagg catccagctc gtacatcagg gcttcctgag taaacggctg gttagatgac
     6961 agcagcgagg cgcgctgttc cctgttcagt tcgactgtcc acatgccgga tatggcatct
     7021 ggctgcaccg gcatccagtc cagcgcctgt gcaatgcctg acgccatcat ctccgtatct
     7081 gtgatacgtt caggacgatg cagcagggcc agcggcgtca gcgcggtttg cgtcagtcgg
     7141 tttgccagca acagcgcggt ggcggattct gccgtccgtt ccgggttctt cggttccaga
     7201 cgcacggaaa tcaccagcag tacggccttt tcctgaataa agttgtccag ccagtcgtcc
     7261 acggcggcca gccccggctc cggggcagaa aacagacggg cttcggggag tccggctttt
     7321 tgccattcgt cctgccagat agttctcacg gaggcccctg aaagcggcgc atcaatttcc
     7381 agtagtacgt tgatatgaaa atcagggggc agcagaaaca attgcggtct gatatcaata
     7441 aaaaggcggg aaataatttc ggataaacgc ccggtaaccg gtttattggt taatacggga
     7501 atgacactgc gacgcacggt atcttctccg cgcaaatcag cgtaagtatt caacacctga
     7561 tgacgcgcca gaaatgctgt tgtagtttct gcgacggaat caccattcag cgcggtttgt
     7621 acggaaaaac tgagtatctg caacgcccgt cgtccccgcc gtgtttcctg caaaatgact
     7681 tcttcgcgac gttgattcca ggcatcggca cgaatatgtt gagtgacaaa taccagccag
     7741 cgcaccgaac cgaccagtga ccagataaaa gcaggcacgc cgcaggccag cccccagaaa
     7801 tatgggccgt tttttgccag ttcatcgctg gcgaataatg aggttattgc cacaccaaca
     7861 gacagcatca gtaataaggc tgacagccat ttaatgcggg ctggcggtgc gggatagggt
     7921 tctgcgtcgg gaatggtatt aagtcggatg ggcattgtaa taatccgtta ttctgaaata
     7981 tgaatacaat gaatctcaag gtgcttgaac tatctgatta attcacttgt ccataatgtt
     8041 tttgctttat taactgtgat ttacctcatc ccatcatatg gtaataaaaa gaaattatcg
     8101 catgcttttt tattgcatcc ccagtaaatc tctttatcat tgctcatcca cattgctgtt
     8161 gcagcagcat tgataacaaa actccattct attccgtcat acattagtct ccctctaatt
     8221 tcacaaggta accaataata cattctgttt atttcgccat aatcatcatg ctttatactt
     8281 agattgaata tgtctataat atctctagaa ttaagcaccc aatgattaca cgcagtaata
     8341 agcaccttat ctgattgatt ggtacctata tatattggtt tattaatgtt tattatctct
     8401 attttttctt cggagtaact atttgaa
//
LOCUS       DAZGHF010000175         8392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGHF010000175 DAZGHF010000000
VERSION     DAZGHF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.176"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..252
                     /locus_tag="ACHK3A_004422"
     CDS             <1..252
                     /locus_tag="ACHK3A_004422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742422.1"
                     /translation="LFYYVCRKKLRKRRTINTQLSHTDWQRKCFQEQVICHGKANQKY
                     RLILVFKPFLLVSMIQRLSKKLGNSTTLGNYGLCPAALI"
     gene            complement(557..646)
                     /locus_tag="ACHK3A_004423"
     tRNA            complement(557..646)
                     /locus_tag="ACHK3A_004423"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(610..612),aa:Ser,seq:cga)
     gene            740..1537
                     /gene="mtfA"
                     /locus_tag="ACHK3A_004424"
     CDS             740..1537
                     /gene="mtfA"
                     /locus_tag="ACHK3A_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO8742423.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1638..1713
                     /locus_tag="ACHK3A_004425"
     tRNA            1638..1713
                     /locus_tag="ACHK3A_004425"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1671..1673,aa:Asn,seq:gtt)
     gene            2026..2799
                     /locus_tag="ACHK3A_004426"
                     /pseudo
     CDS             2026..2799
                     /locus_tag="ACHK3A_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
     gene            2849..3784
                     /locus_tag="ACHK3A_004427"
     CDS             2849..3784
                     /locus_tag="ACHK3A_004427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HFO8742424.1"
                     /translation="MAGPADASQSVLKNNRSSLKGDFTESAELYLVLHDLSGHPINVS
                     EGLEFVQSGTNVSYVQISTIDYTQNLYGEYKATVTGGGEGIATLIPVLNGVHQAGLST
                     TIEFISAGTRPMNGTVSVNGANLPAASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSS
                     TASWVDVDTSGKVTFKNVGDRNAVIITATPRSGGAIYQTQVRVKGWWVNHGNNLMQLS
                     QAENYCSNQVGNGYPLPRADLLSNGHMRREIGSLYGEWGDMGNYMKEADFYSMVYWSS
                     NSAGAGQQYIVSLETGTQNTYQTYEFFYGACYKQI"
     gene            complement(4046..5098)
                     /locus_tag="ACHK3A_004428"
     CDS             complement(4046..5098)
                     /locus_tag="ACHK3A_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO8742425.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKSASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPKYELPLSKDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIEKIQSETDIPIVIVHGPKGEEKARVLVDCYNNVFRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLLRI"
     gene            5413..6729
                     /gene="shiA"
                     /locus_tag="ACHK3A_004429"
     CDS             5413..6729
                     /gene="shiA"
                     /locus_tag="ACHK3A_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO8742426.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            6831..8285
                     /gene="amn"
                     /locus_tag="ACHK3A_004430"
     CDS             6831..8285
                     /gene="amn"
                     /locus_tag="ACHK3A_004430"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO8742427.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         2148 a         1938 c         1934 g         2372 t
ORIGIN      
        1 cttttttatt atgtctgtag aaaaaaactg cgaaaaaggc gaacaataaa tacacaactc
       61 agccatacag actggcagcg caaatgtttc caggagcaag tcatctgtca tggtaaagct
      121 aaccagaaat ataggttaat tttggtgttt aaaccttttt tacttgttag tatgatacaa
      181 aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact ttgtcccgcg
      241 gctttaatct gacggccgcg ttcctttttt ggctcaatta ttgacccctt ctctacgcaa
      301 cttcagttcc cgccagcaac tttgcggcag tttcatagga tcaatgtcta aaagaataat
      361 ggtgatcgat aagaaaacga ctgaataact gtagattttc gctcgaaacc ttcctgtcag
      421 ctccatagcg aatcaagtgc tgaatgtcac agtatcgaac agaaaacagt gacgatctaa
      481 cccttcaaga atattctacg attgttctgt ttaggaaaag caaggcggga agtcgggaga
      541 taagtcattg ataaagtggc ggagagaggg ggatttgaac ccccggtaga gttgccccta
      601 ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc agatggttgc
      661 catgatgcca ggaaatttgg cattttaaca gtccctgtcc gtgcaatttt gttcaagtga
      721 cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat cagcacatca
      781 aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgtgtc tgacggaaca
      841 ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc ggcttgttcc
      901 tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc tattttgcct
      961 gcccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct taatttatcc
     1021 tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc ataaccaacg
     1081 tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact ggttggatat
     1141 acaagattct tttgatgctt ctggttttaa cctgattatt catgaagtcg ctcataagct
     1201 ggacacccgt aacggcgatc gcgccagcgg agttcccttt atttcgttgc gtgaggttgc
     1261 tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa tcgaattagt
     1321 tggtgagaat gcggcgagca ttgatgctta tgctgccagt gatcctgctg aatgttttgc
     1381 cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt tcccttcatt
     1441 gtggcaacgt ttctgtcaat tttatcaaca agatcctttg cagagactgc atcacgctaa
     1501 tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc agattaatta
     1561 accaattgaa atggcttatg aaatttagtg ttgacagaca aggtaccgct aagtaatatg
     1621 cgccccgttc tcacgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
     1681 atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct ttttagcgca
     1741 atgtcactga ccttagttga gcattgcttt ttaacggata gcgggttttt gacatcttaa
     1801 gcgccctcga cctttatggt tgagggcgtt ttgctatgaa cgccatcacc attttcccct
     1861 cgattacaaa acttgagtaa ttcagtagtc tcccctcttg caactcacac ccaaaactgc
     1921 ttaacgaaaa gttattaatt ttcaatcata ttgctatcag gatttacatt ttttcgctgt
     1981 gctagaaagg gcgcatttat gttagctcgt tcagggaagg taagcatggc tacgaagaag
     2041 agaagtggag aagaaataaa tgaccaacaa atcttatgcg ggatgggtat taaactacgc
     2101 cgcttaactg cgggtatctg cctggtaact caacttgtgt tccctatggc tgcggcagca
     2161 caaggtgtgg taaacgccgc aacccaacaa ccagttcctg cacaaatcgc cattgcaaat
     2221 gccaatacgg tgccctatac ccttggagcg ctggaatcgg cccaaagcgt tgccgaacgt
     2281 ttcggtattt cgttggctga gttacgcaaa ctcaaccagt ttcgtacgtt tgctcgaggt
     2341 tttgataatg tccgccaggg tgatgaactg gatgtcccgg cacaagttag tgaaaaaaaa
     2401 ttaaccccgc cgccgggtaa tagcagtgac aaccttgagc aacagatagc cagtacttca
     2461 cagcaaatcg ggtctctgct cgccgaagat atgaacagcg agcaagcggc caatatggcg
     2521 cgtggatggg cctcttctca ggcatctggc gcattgacag actggttaag ccgcttcggt
     2581 accgcaagaa tcacgctggg cgtggatgaa gattttagcc tgaagaattc ccagttcgat
     2641 tttctccatc catggtatga aacgcctgat aatctctttt tcagccagca taccctccac
     2701 cgtactgacg aacgtacgca gattaacaac ggcttgggtt ggcgtcattt cactcccaca
     2761 tggatgtcgg gcatcaactt ctttttcgac cacgatctta agcgcagaaa cgagctatgg
     2821 tggggcgcaa acagtagata taacgctggt ggcaggcccg gcagacgcct cgcagtccgt
     2881 ccttaaaaac aaccggtcat cactgaaagg ggactttacc gaaagtgctg aactatatct
     2941 tgttttacac gatctatcag gtcatccgat caatgtttct gaagggctgg aatttgtgca
     3001 atcaggtacc aacgtgtcct atgtgcaaat cagcacaatt gattacactc aaaatttata
     3061 cggtgagtat aaagccactg ttacaggcgg cggagagggt atcgcaacgc tgatccccgt
     3121 gttgaatggt gttcaccagg cgggtctgag taccacgata gaattcatta gcgcgggaac
     3181 cagaccaatg aacggtacag tatcagtgaa tggtgcaaac ctacctgcag cttcattccc
     3241 ttcgcaggga tttaccgggg cgtattatca gttgaataat gacaactttg caccaggaaa
     3301 aacggcggct gattatgcgt tttcaagcac tgcctcctgg gttgatgttg atacttccgg
     3361 taaggtgaca ttcaaaaatg ttggagatag aaatgctgta ataatcacgg cgaccccaag
     3421 atcaggtggc gctatttacc aaacgcaagt acgggtgaag ggctggtggg tgaatcacgg
     3481 taataacctc atgcaattaa gccaggctga aaactactgc agcaaccagg tcggtaatgg
     3541 ctaccccctt ccccgcgcgg accttttaag caatgggcac atgcggcgcg aaatagggtc
     3601 actgtatggt gagtggggag atatgggaaa ttacatgaag gaagctgatt tttattcaat
     3661 ggtatattgg tcttctaatt ccgcaggcgc aggccaacaa tacattgtat ccctggaaac
     3721 agggacgcaa aacacttatc aaacgtatga atttttttat ggggcgtgtt ataaacagat
     3781 ttaatttttc gttataattt cttacagagt gctccattcg gggcgctctt ttttacattc
     3841 tctctacaga acttgccctt ctctcacaga aaatgaataa ataaaaatgc gataccgcca
     3901 gattcgcgtt cggtgtgact atggttgtat ccggtgagta cagctttgat ccaaaggctg
     3961 gctttctgta atgattgacc agccacaaag tattgatgac accaccgttc ataacagatc
     4021 ggcactacta tgcataagta ctgcctcata ttctcaacaa aacacttttt aatgcctttg
     4081 cgaattcatc cagacttata ttgtcaattt tttgcccaac gacgactgac tccgagacat
     4141 cggccattgc gggccatcgc gttttgtaat cagcataaat tgcaacaacg ggggtattat
     4201 aggcacttgc catatgtaat attgaggtgt caggagttat tgcgatataa gcatctttta
     4261 tgattgctgc cgaacgttta atcgagggtg ataaggataa acggaagaca ttattataac
     4321 aatccactaa caccctggct ttttcttcgc cttttggtcc atgaacgatc actattggta
     4381 tatctgtttc actttgaatt ttttctatta gattttctgc aatcgataat gaaaatgtac
     4441 gttcctgcga gctaccttct aaattaagcg caatgtaaga tcctaacgat cgcatttcct
     4501 cgcgcacctc atcgagtaca tccttactta gtggcaattc atattttggc ctgactgctg
     4561 gaaaacccgc ctcacgcatc ataaatgccc atgtcatggg tacaggagcc cggagattct
     4621 gatccatccg ggacgcgctc ttacacaaag gggaatagca cttcatggtt aaaccaacaa
     4681 cctgaaaatt cgtttttgct ttcaactgac tgataaatat catcgttttc aggttctttt
     4741 tacgcatcgc ctctatacaa agatccggcg taccgtattg ctgtgttatg ttcttcgcta
     4801 gatcttttat ttcttttaat gttgcgtgat cctgcatagt cattgtaact aatgttaatt
     4861 tagtctgttc aagtttgagc gcattaaaga cttctaaatt aattgtcgac gttacaatta
     4921 aaagatgctt aattttatgc aattcaagcg cccgaataac aggaaagatg gccatagcat
     4981 cgccaatctg atcgggaata tggatgacaa caaagtctgt tttttcaata ttgaaattat
     5041 aagctttata atcgtagtaa ctaaatgcaa tacgtctcaa caatgatgct aaaaacatac
     5101 ctaacctcgc ctccctactg gttataatgc aatgcagtct atcagactca tcagggtgcc
     5161 attttgtgca tatgcggact tttatgtttc atatctcgaa cctgtgaggc ctctgcttaa
     5221 tccttaaaca acaccagcaa ctcctgcgct ttcatcttcc agcgaatttt tcatgttgct
     5281 gctcatcagc cataaaatca tttgcagatg cgctctgtcg aggtagtctc ataaggttcg
     5341 tttatagatc gacggcaatg tgagttacct tttccatact aattataaaa agacagtaca
     5401 aacaggatca ttatggactc cacgctcatc tccactcgtc ccgatgaagg gacgctttcg
     5461 ttaagtcgcg cccgacgagc tgcgttaggc agtttcgctg gtgccgtcgt cgactggtat
     5521 gattttttac tctatggcat caccgccgca ctggtgttta atcgcgagtt tttcccgcaa
     5581 gtaagcccgg ctatgggaac gctcgccgca tttgccacct ttggcgtcgg attcctgttc
     5641 cgtccgctcg gcggtgtcat tttcggtcac tttggcgacc gactgggacg taagcgcatg
     5701 ttaatgctga ccgtctggat gatgggcatc gcgacagcct tgattggtat tcttccttca
     5761 ttctcgacca ttgggtggtg ggcacctatt ttgctggtga cactgcgtgc cattcaggga
     5821 tttgccgtcg gcggcgaatg gggaggcgcg gcgttgcttt ccgttgaaag tgcgccgaaa
     5881 aataaaaaag ccttttacag tagcggtgta caagttggct acggtgtagg tttactgctt
     5941 tcaaccggac tggtttcatt gatcagtatg atgacaactg acgaacagtt tttaagctgg
     6001 ggctggcgca ttcctttcct gtttagcatc gtactggtac tgggagcatt gtgggtgcgc
     6061 aatggcatgg aggagtccgc ggaatttgaa caacagcaac ataatcaagc ggccgcgaaa
     6121 aaacgcatcc cggttatcga agctctgtta cgacatcccg gtgctttcct gaagattatt
     6181 gcactacgac tgtgcgagtt gctgacgatg tacattgtta ctgcctttgc acttaattat
     6241 tcaacccaga atatggggtt accgcgcgaa cttttcctta atattggttt gctggtaggt
     6301 ggattaagct gcctgacaat tccctgtttt gcctggcttg ccgatcgttt tgggcggcgc
     6361 agggtttata tcacaggtgc gttaatcgga acgttgagcg catttccttt ctttatggcg
     6421 cttgaagcac aatctatttt ctggatagtt ttcttctcca tcatgctggc aaacattgcg
     6481 catgatatgg tggtgtgtgt acaacaaccg atgtttaccg aaatgtttgg tgccagttat
     6541 cgctatagtg gcgctggagt cggttatcag gttgccagtg tggttggcgg tggatttaca
     6601 ccttttattg ccgctgcact catcacttac tttgccggga actggcatag cgtcgccatt
     6661 tatttgctgg ctggatgcct gatttccgca atgaccgctt tgttgatgaa agacaatcaa
     6721 cgcgcttaat agcctggcga agatcatccg atcttcgcct tacacttttg tttcacattc
     6781 ctgtgacata ctatcggatg tgcggtaatt gtatggaaca ggagacacac atgaataata
     6841 agggctccgg tctgacccca gctcaggcac tggataaact cgacgcgctg tatgagcaat
     6901 ctgtagtcgc tttacgcaac gccattggca aatatattac aagtggcgaa ttacctgatg
     6961 aaaacgcccg caaacaaggt ctttttgtct atccatcact gaccgtaacc tgggacggta
     7021 gcacaaccaa tccccccaaa acgcgcgcat ttggtcgttt tacccacgca ggcagctaca
     7081 ccaccacgat tactcgccct actctctttc gttcgtatct caatgaacaa cttacgttgc
     7141 tgtatcagga ttatggtgcg catatctcgg tgcaaccctc gcagcatgaa atcccttatc
     7201 cttatgtcat cgatggctct gaattgacac ttgatcgctc aatgagcgct gggttaactc
     7261 gctacttccc gacaacagaa ctggcgcaaa ttggcgatga aactgcagac ggcatttatc
     7321 atccaacgga attctccccg ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg
     7381 cgcggttgcg ccattatacc ggtacgccag ttgaacattt tcagccgttc gtcttgttta
     7441 ccaactacac acgttatgtg gatgaattcg ttcgttgggg atgcagccag atcctcgacc
     7501 ctgatagtcc ctacattgcc ctttcttgtg ctggcgggaa ctggatcacc gccgaaaccg
     7561 aagcgccaga agaagccatt tccgaccttg catggaaaaa acaccagatg ccagcatggc
     7621 atttaattac cgccgatggt cagggtatta ctttggtgaa tattggcgtg ggaccgtcaa
     7681 atgctaaaac catctgcgat catctggcag tgctacgccc ggatgtctgg ttgatgattg
     7741 gtcactgtgg cggattacgt gaaagtcagg ccattggcga ttatgtactt gcacacgctt
     7801 atttacgcga tgaccacgtt cttgatgcag ttctcccgcc cgatattcct attccgagca
     7861 ttgcggaagt gcaacgtgcg ctttatgacg ctaccaagtt agtcagcggc aggcctggtg
     7921 aagaagtcaa acagcggtta cgtactggca cggtggtaac cacagatgac aggaactggg
     7981 agttacgtta ctcagcttct gcacttcgtt ttaacttaag ccgggccgta gcaattgata
     8041 tggaaagtgc aaccattgcc gcgcaaggat atcgtttccg cgtgccatac gggacactac
     8101 tgtgtgtttc agataaaccg ttacatggtg agattaaact tcccggccag gctaaccgtt
     8161 tttatgaagg cgctatttcc gaacacctgc aaattggcat tcgggcgatc gatttgctgc
     8221 gcgcagaagg cgaccgactg cattcacgta aattacgaac ctttaatgag ccgccgttcc
     8281 gataataagt tatgaacctt cttatcagaa atagatgtga aggattaagt aagacatctg
     8341 gcaaatagcc tgcaataaac gctggctgtt tgcttccttg ggcagatgcg ag
//
LOCUS       DAZGHF010000176         8389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGHF010000176 DAZGHF010000000
VERSION     DAZGHF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.177"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            83..2461
                     /gene="ppsA"
                     /locus_tag="ACHK3A_004431"
     CDS             83..2461
                     /gene="ppsA"
                     /locus_tag="ACHK3A_004431"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO8742428.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2518..4164)
                     /gene="fadK"
                     /locus_tag="ACHK3A_004432"
     CDS             complement(2518..4164)
                     /gene="fadK"
                     /locus_tag="ACHK3A_004432"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO8742429.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPAACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFIHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEDVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4221..4514)
                     /locus_tag="ACHK3A_004433"
     CDS             complement(4221..4514)
                     /locus_tag="ACHK3A_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO8742430.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(4511..5800)
                     /locus_tag="ACHK3A_004434"
     CDS             complement(4511..5800)
                     /locus_tag="ACHK3A_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8742431.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLDAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5855..6793)
                     /locus_tag="ACHK3A_004435"
     CDS             complement(5855..6793)
                     /locus_tag="ACHK3A_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO8742432.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQTV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSN
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6813..7577)
                     /locus_tag="ACHK3A_004436"
     CDS             complement(6813..7577)
                     /locus_tag="ACHK3A_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO8742433.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            7893..>8389
                     /locus_tag="ACHK3A_004437"
     CDS             7893..>8389
                     /locus_tag="ACHK3A_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO8742434.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENF"
BASE COUNT         1992 a         2175 c         2182 g         2040 t
ORIGIN      
        1 ttttcatccg gttaaatatg caaagataaa tgcgcagaaa tgtgtttctc aaaccgttca
       61 tttatcacaa aaggattgtt cgatgtccaa caatggctcg tcaccgctgg tgctttggta
      121 taaccaactc ggcatgaatg atgtagacag ggttgggggc aaaaatgcct ccctgggtga
      181 aatgattact aacctttccg gaatgggtgt ttccgttccg aatggtttcg ccacaaccgc
      241 cgacgcgttt aaccagtttc tggaccaaag cggcgtaaac cagcgcattt atgaactgct
      301 ggataaaacg gatattgacg atgttaccca gcttgcgaaa gcgggcgcgc aaatccgcca
      361 gtggattatc gacactccct tccagcctga gctggaaaac gccatccgcg aagcctatgc
      421 acagctttcc gccgatgacg aaaacgcctc gtttgcggtg cgttcctccg ctactgcaga
      481 agatatgccg gacgcttctt ttgccggtca gcaggaaact ttcctcaacg ttcagggttt
      541 tgacgccgtt ctcgtggctg tgaaacatgt atttgcttct ctgtttaacg atcgcgccat
      601 ctcttatcgt gtgcaccagg gttacgatca ccgtggcgtg gcgctgtccg ccggtgttca
      661 gcggatggtg cgctccgacc tcgcatcttc tggcgtgatg ttctccattg ataccgaatc
      721 cggctttgac caggtggtgt ttatcacttc cgcatggggc cttggtgaaa tggtcgtgca
      781 gggtgcggtt aacccggatg agttttacgt gcacaaaccg acactggcgg cgaatcgccc
      841 ggctatcgtg cgccgcacca tggggtcgaa aaaaatccgc atggtttacg cgccgaccca
      901 ggagcacggc aagcaggtta aaatcgagga cgtaccgcaa gaacagcgtg acatcttctc
      961 gctgaccaac gaagaagtgc aggaactggc aaaacaggcc gtacaaattg agaaacacta
     1021 cggtcgccca atggatattg agtgggcgaa agatggccac accggtaaac tgttcattgt
     1081 gcaggcgcgt ccggaaaccg tgcgctcacg cggtcaggtc atggagcgtt atacgctgca
     1141 ttcacagggt aagattatcg ccgaaggccg tgctatcggt catcgcatcg gtgcgggtcc
     1201 ggtgaaagtc atccatgaca tcagcgaaat gaaccgcatc gaacctggag acgtgctggt
     1261 cactgacatg accgacccgg actgggaacc gatcatgaag aaagcatctg ccatcgtcac
     1321 caaccgtggc ggtcgtacct gtcacgcggc gatcatcgct cgtgaactcg gcattccggc
     1381 ggtagtgggc tgtggtgatg caacagaacg gatgaaagac ggtgagaacg tcactgtttc
     1441 ttgtgccgaa ggtgataccg gttacgtcta tgcggagttg ctggaattta gcgtgaaaag
     1501 ctccagcgta gaaacgatgc ctgatctgcc gttgaaggtg atgatgaacg tcggtaaccc
     1561 ggaccgtgct ttcgacttcg cctgcctgcc gaacgaaggc gtcggccttg cgcgtctgga
     1621 atttatcatc aaccgtatga ttggcgtcca cccacgcgca ctgcttgagt ttgacgatca
     1681 ggaaccgcag ttgcaaaacg aaatccgcga gatgatgaaa ggttttgatt ctccgcgtga
     1741 attttacgtt gggcgtctga ctgaagggat cgcgacgctg ggtgccgcgt tttatccgaa
     1801 gcgcgtcatt gtccgtctct ctgattttaa atcgaacgaa tatgccaacc tggtcggtgg
     1861 tgagcgttac gagccagatg aagagaaccc gatgctcggc ttccgtggcg cgggccgcta
     1921 tgtttccgac agcttccgcg actgcttcgc gctggagtgt gaagcagtga aacgtgtgcg
     1981 caacgacatg gggctgacca acgttgagat catgatcccg ttcgtgcgta ccgtagatca
     2041 ggcgaaagcg gtggttgagg aactggcgcg tcaggggctg aaacgtggtg agaacgggct
     2101 gaaaatcatc atgatgtgtg aaattccgtc caacgccttg ctggcagagc agttcctcga
     2161 atatttcgac ggcttctcaa ttggctcaaa cgacatgaca cagctggcgc tcggtctgga
     2221 ccgtgactcc ggagtggtgt ctgaactgtt cgatgagcgc aacgatgcgg tgaaagcact
     2281 gctgtcgatg gcgattcgtg ccgcgaagaa acagggcaaa tatgtcggga tttgcggtca
     2341 gggtccgtcc gaccacgaag actttgccgc atggttgatg gaagagggga tcgatagcct
     2401 gtctctgaac ccggataccg tggtgcaaac ctggttaagc ctggctgaac tgaagaaata
     2461 aaataaatcc ccggcggcgt tttgtcgccg ggtttatgtg atccccgaag atgaaactta
     2521 ttcaatctct tcacagacat cctgagttaa tcgccgcata atatctttcc ttaacaaaaa
     2581 cttttgtatt ttacctgagg ctgtgcgcgg tagtttttcg attaccacga tatgttcagg
     2641 atatttatat ttcgcgaccc gtttacggct aaaaaatgcc actacatctt ccagcgataa
     2701 tgaatgatgc ggtgctttca gcacgacata agcgcatgaa cgttcaccta agcgttcatc
     2761 gggcattgca accacacaag catcgtgaat tttaggatgc tgcaataaaa tatcttctac
     2821 ttcacggctg ctaatatttt cgccgccgcg gacaataata tctttcttgc gcccggttat
     2881 ttttatatag ccagcctcat ccatgcggca gagatcgccg ctgtaatacc agccttcttc
     2941 atccagcgca cgggcggtta attcaggttc atcaaaatac cccataaaca cattggggcc
     3001 acgcgaggct tcttcacctt cgcaacctgg cggtaaagtc ttgcgtgcgt catcgaccac
     3061 tttaatctct acacctgcgg cagcgtaacc atcggtgtgt ataaagcgcg acaaaggatc
     3121 atcgagattc accaccgcat gcggcgaact ttctgtggaa ccataaacac ttaataattt
     3181 aatgccgcgc tgctggcatt cacgcgccac ttttttgggg attgtggtac cgccgcaaag
     3241 aaagaaacgc agcgctgaaa ggtctgcggg ctgtttctcc agtaaattca aaagatcata
     3301 gacaaacggc gttgcgccga gcatacaggt gcaacgctgc tgctcaagca gcgcgagaca
     3361 cgcagcagga gtgaaaatat ctaacaacac gctgcgagca ccaatcaaaa aaggtgctgt
     3421 tacgccatgc agaaagcccg ttgcgtgacc aagtggcgca ggcatcataa agacatcctg
     3481 ccaggtcaga tttagccgcg cgcaataagc ccgctcactg gcgagaatat tgttatgcgt
     3541 tagcatcacg ccctttggca gaccctcggt tccggaggta aacagcaccg cggctaattc
     3601 atcgccgtgg accgttatcg ccgtggtcag aggagtattg tcggcgataa tctgacttaa
     3661 tgagagagca gaggtggcgg gagccagttt gtccacgccg acaatttgtt gtagttgtgg
     3721 aagctgattt tgcagcggca ggattaaatc taccggacgc gtttgtttaa acaacgtcgg
     3781 tgcaaagaac atttttgcct gacacttatt gagcacccac accagttctg cttctcgcca
     3841 ggaaggcaac agcggcacgg aaaccgcacc ggttttcagg caggcaagat agataacggt
     3901 aaattcacac cagcctggca gttgaaatgc gatgcgatcg cctgattcaa tacccttcgc
     3961 taacatccag tttgccagac agctcgcggc gtgatcgagc gcgctataag tgtacgttgc
     4021 accatgattg tcgaccacgg caattttgtc tggcatcgca cgagcggtct gctgccagta
     4081 atcggccagt gaagcatcgc cccataaccc ttgctgacga tacgccgcac gacgttgttc
     4141 gttgaacgtt aatgtcactt tcattttgga ctctcgaaac agaacatcag gcccgagatg
     4201 cgggcctgtg ggatgcatga ttagccgtag cgaaattcaa taccgaaggt gcctgcggga
     4261 tattgccact gttcgagaat agtgttgccg cacagcaccc gacaggtgcc gcactccaga
     4321 caaccggcgg aatcgaaatg aatgtttcct gcgtcatcct gcttataaag tccggcaggg
     4381 caggctttca ttaatttacg gaattcattg atatcgggat ttgccgccag aatgatatgc
     4441 gggtggccct catcaacatg gaatttattt acgcctaatt tgatgtcgac gttaaccgta
     4501 gcgttctggc tcatagcgcg gttgctccct taatgccatc tttcagcaag ttgatcagtc
     4561 caattttctt cgcgtgtccc atgatcattt tgcgtaccgg ttggttaggt ttgccgtcga
     4621 tggtgaacat ctcgttcatg atgtcggcga ccattcgtgg gtattggcta aacaggcgcg
     4681 ggttttccat cagcgccggg atcttgcgaa aatgctgcat atcacgcatg acgcagcttt
     4741 gttccagctc acgtttgtat tgcgcaagac tgctggcgga gaaatccgcg cgttctttgg
     4801 cggcgatcac tgttgtggcg gcagcctgag ccgatgcaat ggctaaatcc atgccgcgga
     4861 ccgtaaaacc caaattcagg cagaagcctg cggcgtcacc aacgatcatc acgccatcgt
     4921 taaccagctg cggcaccatt gccagaccgc cctccggcac catatgcgcg gagtattcaa
     4981 gcagtttgcc gccgctaatc agcgggcgaa tggcggggtg ttgtttaaaa tcttccagca
     5041 tttgcggcac acttttttgc gcatgggcga tatcacccaa tccacaaacc agccccaacg
     5101 atacggaatc tttgttggta tagaggaatc cgccgcccat caggccgtca gaaggggaac
     5161 cggcaaacag ccaggcggca ccttcctcgc ccgtaacatt aaagcgatcg ttgatctgtt
     5221 ctggtgtcag gcctataacc tctttaacac caacagcgta atgatgcgga tcggaagcgg
     5281 aaaccattcc cagcgagcgg ccaagcatcg agttaacgcc atcagccaga atcaccacat
     5341 tcgcttcgag aatatcatcc ccggcctgga cgccagtgac cttgtttcct tcacgaacca
     5401 gtgcatcgac gcgtacgccc gggataaact gcgcgcccgc ctgctcggct tgttccatca
     5461 accacgggtc cagacgatta cgcaatacgg tataagacgc gtgttgcgga acatctggtt
     5521 gctcgcggtg aaaatcgagg gtaacggcgc tttcttcggt taagaaggag attttctcgc
     5581 gtgtgacctt gcgttctacc ggcgctgatg ctgcaaagcc tggaataatt gcgtcaagtg
     5641 tgtgggcata aagacgcccg ccggtcatgt ttttacatcc ggcactgtcg ccgcgttcta
     5701 tcaccaggac atccagcccg gctcgcgcca tgaccagtgc ggcaacgcta ccggcaacgc
     5761 ccgcaccgac cacaatggca tcaaatttgt catccgacat gcgttatgct cccttcccgc
     5821 tgcgtttaca gcgggagatg tggttgaaag ggaatcagcg actcaactgg ctaatcaggg
     5881 cagggacgac tttgtagata tcgcccacca gaccgtagtc ggcatagttg aagattggcg
     5941 catttttatc tttattaatg gcgacaatca cttttgcgcc gttgccgcca accatatgct
     6001 ggatctgccc ggagatcccc agcgtcaggt agagatcgga tttcagcaac acgccggaga
     6061 caccgatata acgttcacgc tccatccagt tctcgccttc ggcaattgga cgtgaacagc
     6121 cgacttcagc attcagcacc gccgccagtt cgtggaccat tttcaggtca tcctgcgctg
     6181 ccagaccacg accgacgcca accacacgtt ttgctttgct caggtccacg ctgcttgccg
     6241 ctttcgcacg gcgttcgcga cagagaattt catgacgcgg ggcaacataa ggtaccgttt
     6301 ctgtcggaca ctgattagag gtatcactgg tgcacggttc ctgaacaccg ggcgcaaggg
     6361 taatgatcgc cagcgggcta ttgatctttt cctgtgcgaa cgctaacccg ccatacatcc
     6421 ggtgttcggc gcaaatgtga ccatcgacaa tatccaccgc agtggcatcg ttcaccagcg
     6481 ccgcattcag ttgcacactt aaccgtgcag ccagagcttt accgcgtttc gtcgcggcca
     6541 gcagcaacat agaagggtgt ttatctttca gcagggcagc aatgctttcg gcgtaatttt
     6601 cagtacgttg cagcgcgtcg ttttgcgcaa gaacataaat gcattttggc ccataaggca
     6661 taactgtctg cgcctggtcg gtattttgta caatggcata cacctgttgg ccccattgct
     6721 gagcgccgcc aaacagttca gcataacgtt caggattatc gctaaagacc cagacgctgt
     6781 ttaattgact catcgtttct tctccgtagg cttcagttca gggctttctt cagatgttcc
     6841 gccagctcgg caatggcctc cggcgaatcg ttatcgagaa tgatgtgctt acgttctgtt
     6901 tgcggcggta cgcgaatgcc ggtcagttca gcaagtggtg cgctctggct ccagccaata
     6961 tcacttgcct gccactgatt taccggtttt ttacccgcgc cgaggatggc tttcatcgaa
     7021 ggaatacgtg gtacgttaat atcggaggtg acgcagagca cggctggaac agagagttca
     7081 ataacttcaa catcatcttc aagcgtgcgt tcaatcacca gtgtattgcc ctgacgctga
     7141 atggcactca ctgcattaat caccggaagt tgcagaattt ctccgaccag caagccaacc
     7201 tgctgggcat aaaggtcgcc ggaaccttca ccaaagatca gtaaatcgaa gccgatcttt
     7261 tcaactgctg tcgccagcgc ctttgcggta tcgagaggca gtgcatgctc aagttgtgca
     7321 tcctgcacca tatacaggct gtgcggcccg cgggatagca cgtctttgcg cactttcgag
     7381 ttctgcaaca aagagccgcc gacggtcagc gcggctatct catcgtcatc tgttgcaagc
     7441 tggcttgcag cttcaatggc attgagatcg aactggctga ttttggcgtc ggcattgtcg
     7501 aaattcaggg tatattctgg agtgacaaca atgtcctgtt cttcaggcac cagcttaaag
     7561 caggttatta ttttcatgat ttctcctgtg agtcgcatac ggaaccatct ccgatggaag
     7621 gtaaaaatgg cctgaaaata gtgtgcgata gcggtgcggg gttttcgacg gagcaagctg
     7681 ttttccttgc caacaatctt gcaaaaaaaa tgcgatcgct aaaacaaaag tgcaatttgc
     7741 ggcaatgttg ttattccatg accgtacaat cttgcattca gtgtaaataa tcttgtttag
     7801 ctctcgctaa acgcggttgt ataaccgcaa tacacgccgt attttatccg gcatatattg
     7861 tttttgcttg ctcatggaat agggaggttg tcatgtatca acgctgtttt gataacgcca
     7921 gcgaaacgct gtttgtcgcc ggtaaaacgc cacggctttc acgttttgca tttagcgatg
     7981 atccaaaatg ggagtccgga catcacgttc atgacaatga aaccgagttg atttacgtca
     8041 agaaaggggt agcaaggttt accatcgatt cttcgttata tgtcgcgcat gccgatgaca
     8101 ttgtggtgat agaacgtggc aggttgcatg cggtggcctc tgacgttaac gatccagcaa
     8161 cgacgtgtac ctgtgcgctg tacggctttc agtttcaggg ggctgaggaa aatcagctac
     8221 tgcaaccgca ttcttgcccg gtaattgccg cagggcaggg aaaagaagtc attaaaacct
     8281 tatttaatga gctaagtgtg attttgccgc aaagtaaaaa tagccaaaca tcttcgttat
     8341 gggacgcatt tgcctatacg ttagcaattc tttactatga aaactttaa
//
LOCUS       DAZGHF010000177         8216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGHF010000177 DAZGHF010000000
VERSION     DAZGHF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.178"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1672
                     /locus_tag="ACHK3A_004438"
     CDS             <1..1672
                     /locus_tag="ACHK3A_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO8742435.1"
                     /translation="LNSSISLILASGLMMSTANAADDVKLKATKTNVAFADFTPTEYS
                     TKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAIEAAKKSTPT
                     LAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGIPLEETFLSE
                     LLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQNRGFNYFMG
                     YHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPFMIYLAYSAP
                     HLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQYDNTIILFT
                     SDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKLVSSMDFYPT
                     ALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSHWWDEENTAF
                     WNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLALYDINDLQQT
                     KDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
     gene            1741..1998
                     /locus_tag="ACHK3A_004439"
     CDS             1741..1998
                     /locus_tag="ACHK3A_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742436.1"
                     /translation="MNKHENKKYCSSDEIWCTQRKELIKSVLETKKASDLEKQSTDIA
                     QQYLSETIQFSIDDFIKNIKRKVKSRGSRPPAKRKNTHKGT"
     gene            2219..2434
                     /locus_tag="ACHK3A_004440"
     CDS             2219..2434
                     /locus_tag="ACHK3A_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8742437.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            2608..2865
                     /locus_tag="ACHK3A_004441"
     CDS             2608..2865
                     /locus_tag="ACHK3A_004441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742438.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            2847..3038
                     /locus_tag="ACHK3A_004442"
     CDS             2847..3038
                     /locus_tag="ACHK3A_004442"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8742439.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            3180..4199
                     /locus_tag="ACHK3A_004443"
     CDS             3180..4199
                     /locus_tag="ACHK3A_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO8742440.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            4920..5135
                     /locus_tag="ACHK3A_004444"
                     /pseudo
     CDS             4920..5135
                     /locus_tag="ACHK3A_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            5310..7280
                     /locus_tag="ACHK3A_004445"
     CDS             5310..7280
                     /locus_tag="ACHK3A_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFO8742441.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKSFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            7299..8090
                     /locus_tag="ACHK3A_004446"
     CDS             7299..8090
                     /locus_tag="ACHK3A_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFO8742442.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAANGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         2511 a         1793 c         1897 g         2015 t
ORIGIN      
        1 gcttaactca tcaatatcac ttatattagc atctggtctg atgatgagta ctgcaaatgc
       61 agcagatgat gttaagctca aagcaacaaa aaccaatgtt gcctttgcgg acttcacgcc
      121 gacggaatac agcactaaag gtaagccaaa tatcattgtt ttaacaatgg atgatttagg
      181 gtatggacaa ttgccttttg atgatagttc atttgaccct aaaacgatgg ataaccgaaa
      241 tgttgttgat acatacaaaa taggtattga taaagcaatt gaagctgcaa aaaaatccac
      301 tccaacatta gcggccctga tggacgaagg tgtaaaattc actaacggct atgttgcaca
      361 cggtgtctct ggcccttcac gtgccgcaat cctgacggcg cgagctcctg ctcgcttcgg
      421 tgtatattca aattctgatg cacaggatgg tatccctctg gaagaaacat tcctgtctga
      481 attacttcag aatcatggtt attacactgc agcaatcggt aagtggcact tatcaaaaat
      541 cagcaacgta ggtgtgcctg aagaaaaaca aacccgtgac taccacgata acttcaccac
      601 cttctccgca gaagaatggc aacctcagaa tcgtggcttc aactacttca tgggttatca
      661 cgcatccggc gtttcacttt ataactcccc gtcattgttc cgtaaccgtg aaaacattaa
      721 agctgaaggg tacattagtg atcaattaac tgatgaagca attggtgtaa ttgaccgtgc
      781 aagcaaactg gaacagccat ttatgattta tctggcatac agtgcaccac acctgcctaa
      841 cgatcagcct gcgcctaaaa actatcagga gaaattcaac actggtagca agactgctga
      901 taactattat gcgtcaattt actccgttga ccagggggtt aaaaagatcc tgaatacact
      961 taaggaaaaa ggtcagtacg acaacaccat cattctgttc acctctgata acggtgctgt
     1021 aattgatggt ccactgccac tgaatggtgc gcaggctggt tacaaaggcc aaccgtatcc
     1081 gggtggtatg catacaccta tgttcatgtg gtggaatggc aagctgaaaa cgggtgatta
     1141 tgacaaactg gtttcatcaa tggacttcta tccgaccgct ttagatgccg cagatatcaa
     1201 gatccctgct tctctgaaac tagatggtgt atcattattg ccatatgtac agaaaggagc
     1261 tgataagaaa tctgcagctg gcgaacctca caaatatctg tcctggatta cctcttattc
     1321 tcactggtgg gacgaagaga acactgcatt ctggaacgga tatcataaat tcgtgagaaa
     1381 agagtctgat gattatccgc acaacccaca tacagaagac ttaagccagt ttgcatacac
     1441 tgtaagaagc aacgattatt ctctggttta cacctatgca gagaataaac tagctctgta
     1501 cgatatcaac gatctgcaac aaactaaaga tctctctgca tcccatcctg atgtagttaa
     1561 gaatatgaag gctgttatga ttgacttcat cacgtcaagt caaaaaccga tcagtgatat
     1621 caaccaagcc aaatttgaga atataaaaaa atctttaact aacgtgaaat gataataagc
     1681 agggagtaga tatatctact ccctggatat agggaaataa atcagttaga gcaataaata
     1741 atgaacaaac acgaaaataa aaaatactgt tcatctgacg agatttggtg tacacaacgt
     1801 aaggagttaa tcaaatctgt tcttgaaaca aagaaagctt ctgatttaga aaaacagagt
     1861 acagatatag ctcaacagta tttatcagag acgattcagt tttctattga tgattttatc
     1921 aaaaatataa aaagaaaagt taaatcccga ggttccaggc ctcccgccaa acgtaaaaac
     1981 actcacaagg gaacatagat tcaatgcgtt tatttagaag gtctgtcttc tttttaatat
     2041 taacttatta attggtgcta ttcatataat tctttaaaca gcgaacttat agccaggatt
     2101 caatagatat agtaacagga acatcagtaa gcgatgagat ggtcttcccc ctccttcacg
     2161 acaaccttta attacgtcat gatggataaa ctttaaccac aaaagtaaga gggataccat
     2221 gtctcaacag aaaatcaccg gcatcgatct ggctaaaacg aacttttatc ttttcagtat
     2281 caatgctcat ggcaaacccg caggaaggat caaactttct cgcaatcaac tgcttaactg
     2341 gctggtgcag cagcctaaaa tgactgttgc gatggaggct cgcgggcatc ccatcactgg
     2401 gccagggaaa tcaggaaact cgaccatgat gtaatatttc ttccggcgca acatgttaaa
     2461 gcttatcagc gacgccagaa aaatgattat gacgacgctc aggcaatagc tgaggcctgc
     2521 caacatggaa ctatacgccc cgtaccaata aaaactctgg agtaacagga cgtccagact
     2581 tttttgaaaa tgcgccggtt ggtttcgatg gaaaggacac aactgataaa tcacgttcgt
     2641 ggacttcttg ctgaatatgg cattgtgttc agtaaaggcg caacagaatt acgccaaaaa
     2701 ttaccggcat tacttgagga tgcggaaaac gaactcactg ataccatgaa gacgttattg
     2761 catcggcagt atatacggtt gattacacta gataatgaac tggagtggta tgattctgaa
     2821 ctgaaaaata tgtccgccaa gatcctatgt gcaaacgctt gttagccatt cctagattca
     2881 gccctctggt tagtcaggca ataaaaagct ggatggggga tggaaaacag tttagacgag
     2941 gaagagatgc atctgcgact ctgggactgg ttccccgaca gttcagcaca ggtgggaagc
     3001 aggttcttct gggtataaca aaatttggag aaatgtaatg aacacatccc accccattaa
     3061 gtcaggtgct ctgtgatgga tgaagggaaa tactggaagg tgtggagcct cgcagatttc
     3121 cggcatcata gtgcccaata caggatgcat aaagctccct cagactggag actccacaaa
     3181 tgaaatatac accggttggc gttgatatcg caaaacatct gattcaggtt catttcatcg
     3241 acgagcacac aggtgaagtg attgataagc aactacgtcg tcaggatttt ctgatgttct
     3301 tctgtaatcg ggaaccctgc ctgattggta tggaggcctg tagaggctct cagcactggg
     3361 ggcgtgaact gacaaagctg gggcataaag tccggttgtt acaggcccac tttgttaagg
     3421 cattcgtcat gggaaataag aatgatgtga tggatgcccg ggctatctgg atggctgttc
     3481 agcagccagg taaggacatc gcggttaaaa cagaagaaca acagtcagtg ctggttttgc
     3541 accgttcccg cagacaattg gttaagttcc gcaccatgca gattaattct ctgcacggca
     3601 cgctcctaga atttggtaaa accattcaca aaggtcgggc agcgatggag cgagagctcc
     3661 ctgaagcact ggagcggatg aaagagagac tgccgccgta tctcattact gtgctggaaa
     3721 accagtacaa ccggctgaat gagctggact cgctgataga ggatattgaa aaaaagctta
     3781 ccagcgtggc gagacagaat gaaacctgta agcggttgct ggatattccc ggcgttgggc
     3841 cacttattgc aacggcagcg gtggccacca tgagggaagc atcagcgttt aaatcagggc
     3901 gagagttcgc ggcttatgtt ggtctggtgc ccaaacagac tggctcagga gggaaaatcc
     3961 gcctgctgga gataagcaaa cgtggtaata catatctgcg agccttgttt attcatagcg
     4021 caagggcggc agcattactg cccaaagaac ctggtccgtg gataaccgaa ctgaaaaaac
     4081 gtcgtccaac cagcgtggca atcgtcgcca tggcaaacaa actagcacga acagtatggg
     4141 cgatagcagc tcatggaagt aaatacgaca agaatcatgt cagtatcaga tcatattaat
     4201 cgctgatacc gataaatatt aacttttaat gaaaggatga atactgaaag gtaactctgg
     4261 cggccatagt gatgacaacg acaggtaaga ccgggactca ctaaacctga atattgtgtt
     4321 gagctaaaag ctcgctggag aaatgaggtg tgagtcagcg aattacatag gggctcgcag
     4381 cttcatggct gcaaaaaagc cggatataaa gctgcaacct acccgtcact gtcaaggcaa
     4441 tggatgcctt gcaacgggat gtgttcatat aatcaatatg tcagatctat gctgatacag
     4501 ggtgccagag cggtattgtt cagagcctca gagaaaactg attcacttcg tgtgtgggtt
     4561 aattgtattc gggaaaagcg tggcttcaac caggcagttg ttgctcttgc aaataaactc
     4621 cttcggatcg cctgggcaat tatcgcccgc aatgagatgt atttgcccgc agcaaaacaa
     4681 aatcaataat ctacgtaaaa tcagtaggag taaagcttac cctgaaattg caaagaatgc
     4741 agaatgatga aaaacaggtc agaccgacgt tacgaaaacc tgcaagatga gccaggccag
     4801 taaggccgag taagcgataa ggtcggagcg cgcgaatgtc tcatcaaggc cagaagcaat
     4861 aacttcatga acaaaccgga tatacgaaag taaacctgtc cctatcatag ttgctgtact
     4921 cttgcaatac gggggaagac catctcatcg cttacgaaca attcgtgaaa acgatgaaga
     4981 aagactacat cgcgtgcatg ccgaaatcta atgtaagaac ggcattgcat aatcttgcag
     5041 tggagatcgg gtattacaat gaaaaccatc cgcatagtgc gttaggttat ctcccaccaa
     5101 gggaatatca acgtcagcga ataacgtcac cttagatgca gtagttgtca ggaaatagtg
     5161 actcaagggc aggcttacca tatacactct tagtggttcg actgggtatt agaatagcta
     5221 ttaacagatt agcactgaac actgcgtaat agtggttgca caaacaaaat gttatgttat
     5281 aacattgctg tattgacata tattttcgaa tgtatatgaa cgtaatcaga actgtcattt
     5341 gtacattaat catacttccg gtggggttac aggcagcgac aagtcattct tctatggcta
     5401 aagatacaat caccgttgtc gcgacaggaa atcagaacac ggtatttgaa acgccgtcga
     5461 tggtcagtgt catcacgaat gacacgccat ggagtcagaa tgcggtcaca tcagccggta
     5521 tgctgaaagg tgttgcgggt ctcagccaga ctggtgcagg acggaccaat gggcagacct
     5581 ttaatctacg cggctatgac aaaagcgggg tacttgttct tgttgacggc gtccgtcaac
     5641 tcagtgacat ggcaaaaagc agtggcactt atctggatcc ggcactcatc aaacgcatcg
     5701 aagttgtccg cggaccaaac tccagtctgt acggcagtgg cgggctgggg ggggtggtgg
     5761 acttcagaac tgccgatgct gcagattttc ttccccccgg agagacaaat ggtgtaagtc
     5821 tgtggggaaa tatcgccagt ggtgaccaca gcacaggctc ggggctcacc tggttcggta
     5881 aaaccggaaa aacagatgcg cttctttctg tcattatgcg taaaagagga aatatctatc
     5941 agagtgatgg tgagcgtgca cctaacaagg aaaaacctgc agccctgttt gcgaaaggct
     6001 ctgtcagtat aacagacagt aacaaagcag gtgccagctt gcgtctctac cagaataaca
     6061 ccactgaacc gggcaatccc actctgacac atggtgacaa cggcctgcgt gacagaaaaa
     6121 cagcacaaaa tgacgtgcag ttctggtacc agtactcccc tgcggataac agcctcatca
     6181 atgtaaagtc aacgttatat ctcagtgata tcactatcaa gacaaacggt cacaacaaaa
     6241 cagcagaatg gcgaaacaac agaacctccg gtgttaatgt tgtcaacagg agccatactc
     6301 tgatttttcc gggagcccat cagttaagtt atggcgccga atattaccgt cagcagcaga
     6361 agccggaagg ctctgccaca ctatatccgg aaggaaacat tgactttaca tcgttgtatt
     6421 tccaggatga aatgacaatg aaaagcaacc cggttaacat tatcgtcggt tcccgttatg
     6481 accggtacaa gagcttcaat ccccgagccg gaaaactgaa agccgaacgc ctgtccccca
     6541 gggcggcgat ttcagtctca ccaacagact ggctgatgat gtacggctcc atatcctctg
     6601 cattccgtgc ccccacaatg gcagaaatgt acagggatga tgtacatttt taccgcaagg
     6661 gtaaacccaa ttactgggta cctaacctta atctgaaacc agaaaataac attacccgtg
     6721 agattggcgc aggtattcaa ctggacggcc tgcttacaga caatgaccgg ctgcagttga
     6781 aaggcggata tttcggaacg gatgccagaa actatattgc cacgcgcgtg gatatgaaac
     6841 ggatgcgttc ttattcttat aatgtatccc gggcccgtat ctggggatgg gatatgcagg
     6901 gtaattacca gtctgattat tttgactgga tgctttctta taaccgcacg gaaagtatgg
     6961 atgccagtag cagggaatgg ctgggctccg gcaatcctga cacactcatc agtgacatca
     7021 gtatacctgt tggtcatgga ggagtttctg ccggatggcg tgctgaactt tcggcagcag
     7081 ccacgcatgt gaaaaaaggc gatccccatc aggctggcta tgccgtacat tccttttcac
     7141 tgtcttataa gcctgtaagt gttaaaagct ttgaggcatc agtgactctg gataatgcct
     7201 tcaacaagct tgccatgaat ggcaaaggtg tgccgctttc aggcagaact gtcagtcttt
     7261 atacccgtta tcagtggtaa cctcgcagga aataaatcat gatgaaaaat acaggctata
     7321 tcttagctct ttgtctgaca gcatcggagc atgccctggc ccacgatgtc tggattaccg
     7381 gtaaacaggc agagaacaac gttaccgcag agattggtta tggtcataat ttcccctcaa
     7441 agggcacaat tcctgacaga aggaatttct ttgaaaatcc ccggctttat aacgggaaag
     7501 agataataac gctgaagcca acgtccacgg attatgtcta taaaactgag tctgcagcca
     7561 acggtaatag ctacgttctg tcaacgaaaa tgaaaccggg atactggtcg agaacctcgt
     7621 caggatggaa gcctgtcagt cgggagggca gaaatgatgt ggcatactgt gagtttgtga
     7681 ctaaatatgc aaaatctttt attcctggtg aacagcagat gccagcacaa ctctatcagt
     7741 ctccaacagg ggatgagctt gaaatcattc cattatccga tatcagtcgt ttcggtgaag
     7801 atgtgaagct gaaagttctg tataaaacat ccccgcttgc cggagctact ctggagcttg
     7861 actctgtcag ttatctgaaa tcatcccgtc atacccatgc agctgagcat aaacattcgg
     7921 ctcataaagc agaacttacc tttgtaagta atgaggacgg tatcatcact gtaccttccc
     7981 ttcatgtcgg gcaatggctg gctaaagtca aaaataagaa agtatttccg gacaagagtc
     8041 tgtgtgatga aactgtcgat gtggcaacct taagcttctc ccgaaattaa taatactatt
     8101 cctgccctga tttatgggca gggtattatc aatacgagaa tagcctaccg acatccgtgt
     8161 aaaaaaccac ataatgattt ttcatatcca tttccagata tacttatgta aagatc
//
LOCUS       DAZGHF010000178         8195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGHF010000178 DAZGHF010000000
VERSION     DAZGHF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.179"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..240
                     /locus_tag="ACHK3A_004447"
     CDS             <1..240
                     /locus_tag="ACHK3A_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO8742443.1"
                     /translation="SVAAAKSGKLPEAFFWTDAENNDVEVTAEELIALSEAAEQAMFT
                     KGMEIHVRQRTMKKELEKLTSADEILAYRVGWAQA"
     gene            316..498
                     /locus_tag="ACHK3A_004448"
     CDS             316..498
                     /locus_tag="ACHK3A_004448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742444.1"
                     /translation="MTMLVYGVKTATEMLILYVMLILFPEHPVDVNNFQIFGSESLDI
                     SIYATVDVFCGAWQCD"
     gene            complement(1443..1721)
                     /locus_tag="ACHK3A_004449"
     CDS             complement(1443..1721)
                     /locus_tag="ACHK3A_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HFO8742445.1"
                     /translation="MRSLFALLITVISCTTASAAMTETCQKYFDETDKFLSQMKTTGV
                     AEGQINLMKQQYEQSKKQFSSLPEASQDAACKQASDALKQSMSAAGIK"
     gene            complement(2475..2723)
                     /locus_tag="ACHK3A_004450"
     CDS             complement(2475..2723)
                     /locus_tag="ACHK3A_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO8742446.1"
                     /translation="MRVEICIAKEKISKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIH"
     gene            complement(2949..3044)
                     /locus_tag="ACHK3A_004451"
                     /pseudo
     CDS             complement(2949..3044)
                     /locus_tag="ACHK3A_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3041..3607)
                     /gene="yecD"
                     /locus_tag="ACHK3A_004452"
     CDS             complement(3041..3607)
                     /gene="yecD"
                     /locus_tag="ACHK3A_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO8742447.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            3917..5689
                     /gene="aspS"
                     /locus_tag="ACHK3A_004453"
     CDS             3917..5689
                     /gene="aspS"
                     /locus_tag="ACHK3A_004453"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO8742448.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKDENN"
     gene            5807..6259
                     /gene="nudB"
                     /locus_tag="ACHK3A_004454"
     CDS             5807..6259
                     /gene="nudB"
                     /locus_tag="ACHK3A_004454"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO8742449.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            6288..7028
                     /gene="yebC"
                     /locus_tag="ACHK3A_004455"
     CDS             6288..7028
                     /gene="yebC"
                     /locus_tag="ACHK3A_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8742450.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            7063..7584
                     /gene="ruvC"
                     /locus_tag="ACHK3A_004456"
     CDS             7063..7584
                     /gene="ruvC"
                     /locus_tag="ACHK3A_004456"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO8742451.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(7586..8188)
                     /gene="yebB"
                     /locus_tag="ACHK3A_004457"
     CDS             complement(7586..8188)
                     /gene="yebB"
                     /locus_tag="ACHK3A_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO8742452.1"
                     /translation="MNVNYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         2139 a         1846 c         2094 g         2116 t
ORIGIN      
        1 tcggtggcgg cagcgaaatc ggggaaactg ccggaggcgt ttttctggac ggatgcggaa
       61 aataatgatg tggaggtgac agcagaagag cttatagcgc tgagtgaagc ggcagagcag
      121 gcgatgttca ccaaagggat ggaaatccac gttcgccagc gcaccatgaa gaaggagctg
      181 gaaaagctga ccagtgcgga tgagatactg gcatacagag ttggctgggc acaggcgtaa
      241 taaaaaaggg gggctgataa acagcccctg accaaagaaa agtatcagcg gaaacattta
      301 gaaggatcta aataaatgac catgcttgta tacggcgtaa agactgccac ggaaatgtta
      361 attctgtacg tgatgctgat cttgtttccg gagcatcctg ttgatgtgaa taattttcag
      421 attttcggaa gtgagagttt ggatatttcc atatatgcca cagttgatgt gttttgtggc
      481 gcctggcagt gtgattaagg gaacagggat aacgaaaaaa gcgggggtat caccaccccc
      541 cggtacaagg aagtattaac tataatggaa acattaatga agacattcag tgctatgtta
      601 tatggcaaat aaagactgcc acagggaagt ttgcagaaac agtgatgccg atcgtgtttc
      661 tgtgctcttc tctgataaga gtaatttttc agatttccgg aagtccgtat tttgcacggg
      721 cccggttgta tgcttcagac tgcggtggat atcaaggtca cggagttggt cggttgcaag
      781 gaatagattg agaattgagc gtgtttctgg cacatgcctg actgtaatca tccctacaaa
      841 ctacattgat gccagaaacg cactggtaac agggcatcgt gtatttcagt atgtccagtt
      901 tttgttgcgg tgattgcata aaaaatcccc cttgaggggt gaaatatcac cggaattttc
      961 ggcaagtatt tttatcgggt tgagtgtatt tctggggctc tgctgaggca attcactgaa
     1021 tgtggctttt gtgctcgctg gaaatgcact catacacttt ttaacgcgat ttttttcagg
     1081 caatagtttt taatttgtta gcgttctcgc tgtcaggcac gttttgagca cgtttccgac
     1141 tctcagctac agcaatccac gactagggac agcacttacg ttttacttag cgcaccgtaa
     1201 aactccgctc ttcagggtaa gggcttacat gaagaaaaaa aataaacttg tgaatatgaa
     1261 agtaaatact ataaaaagat ggttcctctt ttttctcacg tgctgaataa tataaacaat
     1321 agtgcatgat acaaaagaca ctaataacca caaagttatg atacggagca cgatttatgt
     1381 cttcctgatt cagtgtgtca agaaacagtt atcaatatac aataactgtt tcttttatta
     1441 tattatttaa ttccggcagc gctcattgat tgcttaagcg cgtcacttgc ttgtttacag
     1501 gcagcatcct ggcttgcctc gggcagagat gagaattgtt tctttgattg ttcatattgt
     1561 tgtttcatta aattaatctg tccttctgct actccggttg ttttcatttg cgataagaac
     1621 ttatctgttt catcgaaata cttctgacat gtttctgtca tagccgcact ggcagttgtg
     1681 caagatatta cagtgattaa taaagcaaag agacttctca tatatcctcc attgttttgt
     1741 ttgaaaaatt aacgctatta aataactcat agacaggtaa atttatgtga ttatagtcat
     1801 aaactataaa aatattgatg ttgtctttgt agaaaggttg tgagcaatta tgaaaattta
     1861 tttgcttgtt cactggtttt agggagtttg atgtgtccat gaagcgtttc tgacgagaaa
     1921 tcaacataaa tcaccagttg gtgaactagg attgccagaa acgctcctgt cagttctgta
     1981 gtgtgtagtg ctcacttaga gcctggtgaa ctggataatg agtcgcacct cagtatcctg
     2041 caatgggctg cttttcccct gctcctgatt cttttttgtt tcaggtctta ccaacataat
     2101 ggttgttgta ataaattgcg tgtgattctg tatcgggtca accatgtgac gatacatgtt
     2161 cagtaaggaa gactgaaggg ttcagagtgc gagtatgatt aatagatgga atcagcgttt
     2221 caaagatttt gagggaatat aaggtctgac ctgatgatta ccttttgcgc ctgtatcctt
     2281 tgcataaagg aggttgatgt agataaagtc atctccactt gtgatataaa acagtcagtt
     2341 atgaaaaatc ggcataaaca tccttttgtt aaccttaaag ccagacttac ggtttttcca
     2401 tagttacaga taagcggcta gtaaggtttt acaggtgata agcctgtaac ggaaatcacc
     2461 tgcaaatcta ttaattagtg aataaaccag tcatccgctg tttcccagac ttcctgaagc
     2521 gtttcttgta caaactcttg cgcggattct ttatccacgg ctcttaaaat agtgagtcca
     2581 tcattgctgg cagatttaac gataacatct acatcgtcat aacgcttacc gacacgtcgt
     2641 agcatttcct gttgcagggc aggaacagaa ccttttggca ttttgctgat tttttcttta
     2701 gcgatacaga tctcaacacg cataacaccc tcctgtaact gtggttatgt acaggtgtta
     2761 tttttatctg tatggataac cagtgtcaat tcctgatgtt gtttatgggg catcaatggg
     2821 gcatgtatgg gacacttttt atcggcgaaa ttcgtcgaag ttcgtcgaca tggtaaacga
     2881 atcatctatc caacccttga aaaacggcgc tcctggacga tcttcgtcga tttttaaaaa
     2941 tgttgcgtca cgcagttaaa gtggcgggca tactcttcaa ggttggtgat ccccaaccgc
     3001 acccatttag gatgcgacca ttgcggcaga ccgatgtaaa tcataacgcg tggaggatct
     3061 cttccacact gcgcacacgg gcgatgcgcg ggtagatatg attaatgctg ttattgtgct
     3121 gctcggcgct ggcggcgcta caggcatctt cggcaatcac cagattaaaa ccgagttccc
     3181 aggcattgcg ggcggtggat tcaacaccga tattggtcga gatcccacat aacactattg
     3241 tatcgatacc ccggcggcgt aattgcaact ccagatccgt accgtaaaac gcaccccatt
     3301 gacgtttgat gatttcgata tcgctgtcgg ttgcacctaa tgcagcagga tgttgccacc
     3361 agttttcggg caacactttt gcgggggagg gggcatcaac cggctgtttt aatgcttcgg
     3421 cgtaatcggc agaccagcca acgcgcacca gaaacacggg ctgaccgctg gcgcgaaatt
     3481 tcgccgccag cttcccggcg cgattaacca cctcatcggc agtatgtgga cctccggcaa
     3541 aaggtaagat gccttcttgt aaatcaatca ccaccagcgc ggtggtttta gcattaagtt
     3601 caagcatgat gactcctgac aaaacaaaat cgtgcctctc accttaatgc gattagccga
     3661 tggcggaacg acaaattttg ttaattttta tgagttcggg caacaccttt atagcaaact
     3721 ggcgtagagt ctgatttcag gcccgatata aagtggtaac gaggcgttcc agcgcgggcc
     3781 aggctcttac ggtgcggcag aatttccagt ataatagccg ccttttttca ttcagttgtg
     3841 acatacagct aacgctgcga cttggtcacc tgcgcagcag ggggcatccg ccggcagcta
     3901 agttaaggga tatctcatgc gtacagaata ttgtggacag ctccgtttgt cccacgtggg
     3961 gcagcaggtg actctgtgtg gttgggtcaa ccgtcgtcgt gatcttggta gcctgatctt
     4021 catcgatatg cgcgaccgcg aaggtatcgt gcaggtattt ttcgatccgg atcgtgcgga
     4081 cgcgttaaag ctggcctctg aactgcgtaa tgagttctgc attcaggtca cgggcaccgt
     4141 acgtgcgcgt gacgaaaaaa atattaaccg cgatatggcg actggcgaaa tcgaagtact
     4201 ggcgtcctcg ctgactatca tcaatcgtgc agatgttctg ccgcttgact ctaaccacgt
     4261 caacaccgaa gaagcgcgtc tgaaataccg ctacctcgac ctgcgtcgcc cggaaatggc
     4321 tcagcgcctg aaaacccgcg ctaaaatcac cagcctggtg cgccgtttta tggatgacca
     4381 cggcttcctc gacatcgaaa ctccgatgct gaccaaagcc acgccggaag gcgcgcgtga
     4441 ctacctggtg ccttctcgtg tgcacaaagg taaattctac gcgctgccgc aatccccgca
     4501 gttgttcaaa cagctactga tgatgtccgg ctttgaccgc tactatcaga tcgttaaatg
     4561 cttccgtgac gaagacctgc gtgctgaccg tcagcctgaa tttactcaga tcgatgtgga
     4621 aacttctttc atgaccgcgc cgcaagtgcg tgaagtgatg gaagcgctgg tgcgtcatct
     4681 gtggctggaa gtgaagggcg tggatctggg cgatttcccg gtaatgacct ttgcagaagc
     4741 agaacgccgt tatggttctg ataaaccgga tctgcgtaac ccgatggaac tgaccgacgt
     4801 tgccgatctg ctgaaatctg ttgagtttgc agtatttgca ggtccggcga acgatccgaa
     4861 aggccgtgtt gccgctctgc gtgttccggg cggcgcatcg ttgacccgta agcagatcga
     4921 cgaatacggt aacttcgtta aaatctacgg cgcgaaaggt ctggcttaca tcaaagttaa
     4981 cgaacgcgcg aaaggtctgg aaggtatcaa cagcccggta gcgaagttcc ttaatgcaga
     5041 aatcatcgaa gccatcctgg atcgtactgc cgcgcaagat ggcgatatga ttttcttcgg
     5101 tgccgacaac aagaaaattg ttgccgacgc gatgggcgca ctgcgcctga aagtgggtaa
     5161 agaccttggt ctgaccgacg aaagcaaatg ggcaccgctg tgggttatcg acttcccgat
     5221 gtttgaagac gacggtgaag gcggcctgac ggcaatgcac catccgttca cctcaccgaa
     5281 agacatgact gctgcagaac tgaaagctgc accagaaaat gcggtggcga acgcttacga
     5341 tatggtcatc aatggttacg aagtgggcgg tggttctgtg cgtatccata atggtgatat
     5401 gcagcagacg gtgtttggta ttctgggtat caacgaagag gaacagcgcg agaaattcgg
     5461 cttcctgctc gacgccctga aatacggtac tccgccgcac gcaggtctgg cattcggtct
     5521 tgaccgtctg accatgctgc tgaccggcac cgacaatatc cgtgacgtta tcgccttccc
     5581 gaaaaccacg gcggcagcgt gtctgatgac tgaagcaccg agctttgcta acccgactgc
     5641 actggctgag ctgagcattc aggttgtgaa gaaggacgag aataactgat atgactcaaa
     5701 tacacgaaat cattcgcgtt gcatcgaggc ggcaactgag tgaactccca tgagcataga
     5761 taactatgtg aatgggatga gcgaaggcag tcaacgaaga ggcagcgtga aggataaagt
     5821 gtataagcgt cccgtttcga tcttagtggt catctatgca caagatacga aacgggtgct
     5881 gatgttgcag cggcgtgacg atcccgattt ctggcagtcg gtaaccggca gcgtggaaga
     5941 gggtgaaacc gcgccgcaag ctgccatgcg cgaagtaaag gaagaggtca ccattgatgt
     6001 tgtcgctgaa caactgacct taattgactg tcagcgcacg gtagagtttg aaattttttc
     6061 acatttacgt catcgctatg cgccgggcgt gacgcgtaat acggaatcat ggttctgtct
     6121 tgcgctcccg cacgagcgcc agatcgtttt cactgaacat ctggcttaca agtggcttga
     6181 cgcgcctgct gctgcggcgc tcactaagtc ctggagcaac cggcaggcga ttgaacagtt
     6241 tgtaattaac gctgcctgaa caggcagggc tatctggaga tatttttatg gcaggtcata
     6301 gtaaatgggc caacaccaga catcgtaaag ctgcgcagga tgctaagcgc ggtaaaatct
     6361 tcactaaaat cattcgtgag ctggtaaccg cggctaagct gggcggtggt gatccggacg
     6421 ctaacccgcg tctgcgtgca gcaattgata aagcactgtc taacaacatg acccgtgaca
     6481 ctctgaaccg cgcaattgca cgaggtgtgg gcggtgatga tgatgcaaac atggaaacca
     6541 tcatctacga aggttacggt cctggcggca cggcaatcat gattgaatgt ctgtctgaca
     6601 accgcaaccg taccgttgct gaagtgcgcc atgcattcag taaatgtggc ggtaacctcg
     6661 gtactgatgg ttccgtagct tatctgttca gcaaaaaagg cgtgatctcc ttcgagaaag
     6721 gcgatgaaga caccatcatg gaagcagccc tggaagccgg tgctgaagac gttgtgacct
     6781 atgatgacgg cgcgattgat gtctacactg catgggaaga aatgggcaaa gtacgcgacg
     6841 ctctggaagc ggcaggtctg aaagcagaca gcgcggaagt ttccatgatc ccgtccacca
     6901 aagctgatat ggatgcagaa accgcaccga aactgatgcg tctgatcgat atgctggaag
     6961 attgcgacga cgtgcaggaa gtttaccata acggtgaaat ctctgatgag gttgcagcga
     7021 ctctctgatg aggcctgcta aacagcaaaa cggagacgcg tgatggctat tattctcggc
     7081 attgacccgg gttcgcgcgt gaccggctat ggcgtcatcc gccaggtagg caggcaactg
     7141 tcctacctgg gtagcggatg catacgcacc aaagtggatg atttaccgtc tcgtctgaag
     7201 ctcatctatg cgggcgtgac ggaaatcatc acccagttcc agcctgatta tttcgccatt
     7261 gaacaagtct ttatggcgaa gaacgctgac tcagccctga aactgggtca ggcgcgcggc
     7321 gtggcgattg tggcagcggt gaatcaggaa ttgccggtat ttgaatatgc ggcacgtcag
     7381 gtaaagcaaa cggtggtagg tattggtagt gccgaaaaaa gccaggtgca gcatatggtc
     7441 cgcaccttgc tgaaactgcc cgctaatcca caggcggatg ctgccgatgc attagctatc
     7501 gcgattaccc attgtcacgt cagtcagaat gcgatgcaga tgagcgaatc gcggctgaac
     7561 ctggcgagag ggcgactgcg ttaagttata ccgcctcggt cagttccggc tgaggcgttt
     7621 ccactccctc cgcatgaatc aacaccaaac ccggatgatg ccacaaactg gctggcgtga
     7681 cggttttacg ctcccacgga atagaaccta agaaccagaa tttccagaaa gtgagttttg
     7741 cattcggatt gctatttaac aattctccaa acgtctctat ttcacccacc ggaatacata
     7801 gcgcttcttt ataaatatca aaaacaaatt ttgaacagaa ctggcgcgaa gattcgtatt
     7861 taaaaccggt gtggtaaagt ttgcgtagcc gggagggaac ctgttcaaca attcgttgtt
     7921 tttgttgttc tgtcagccca gcgtctaatc gctttatagc atagcgttga ttagacgaac
     7981 gtttaataaa acgagatagc gtggtgatgg ttgaaagggg aacccggctt tctgcaacca
     8041 ggaagtcttc accgttatga ccgataatga tcccgacgtg attactccag caatttgatg
     8101 cagctgatat ttgaccaaat aaggtagcac ctatacatgt aaagacgata tcaccaattt
     8161 catattcagc aggataatta acattcacgt tgtca
//
LOCUS       DAZGHF010000179         8181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGHF010000179 DAZGHF010000000
VERSION     DAZGHF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.180"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            307..1290
                     /locus_tag="ACHK3A_004458"
     CDS             307..1290
                     /locus_tag="ACHK3A_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFO8742453.1"
                     /translation="MPEKYSPDDQSSTIDPYLITSVRQTLEEQGAALQNLSKQLDSGQ
                     YQCVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PCDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1362..2510
                     /locus_tag="ACHK3A_004459"
     CDS             1362..2510
                     /locus_tag="ACHK3A_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFO8742454.1"
                     /translation="MLIKVKSAVSWMRAHLSAISLSDIQKHLVKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2534..4210
                     /locus_tag="ACHK3A_004460"
     CDS             2534..4210
                     /locus_tag="ACHK3A_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFO8742455.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDISADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4220..4960
                     /gene="kdsB"
                     /locus_tag="ACHK3A_004461"
     CDS             4220..4960
                     /gene="kdsB"
                     /locus_tag="ACHK3A_004461"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO8742456.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEAAEPSTVKVVVNTHQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4957..6984
                     /locus_tag="ACHK3A_004462"
     CDS             4957..6984
                     /locus_tag="ACHK3A_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFO8742457.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLSVNGAPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISKAREAMHTIVTGDLSKYNLAPAFVADESECSDIVLVVDQTFND
                     MSVTYGNAGQNEFSAMLEAAMAENPQATIWVKVHPDVLKGKKTGYFADLRATQRVRLI
                     TENISPQSLLRHISRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSVTLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLLRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLRTATNRLSYSRRCTAATACVVWGVKGEQQWQVEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPSLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKGKKVILVPGQVEDDSSIKTGTISIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAELIAELADYQALDADIIQCIQHADEVHTMT
                     SLSGFEALLHGKHVHCYGLPFYAGWGLTFDEHHCGRRERELTLADLLYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTSRKPMFITRKKAGWVIRYYRKLIMFCKVRFG"
     gene            7019..>8181
                     /locus_tag="ACHK3A_004463"
     CDS             7019..>8181
                     /locus_tag="ACHK3A_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFO8742458.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDCFYCRHRHYQAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKYWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRSYWRKQLYKVTQRKVLPR
                     LMNELDQLYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGGRVIYVHDLPMPGLLRHAKAVVTINSTVGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYILMKTQLNAV"
BASE COUNT         2109 a         1913 c         2104 g         2055 t
ORIGIN      
        1 tatttgttgg ctttagttat ttcaggtttt attaagaaag aagctaataa tatacatcat
       61 gttgttattt gttggtttaa tgttaacatg tatgataaaa atcatgtggg acgggcatgt
      121 gcccctccct ttcctttatt atgtctgata atgaaatgct aaaattattt ttgtttgtag
      181 actgtgttat atgctttatg gaaaagtagt attttgattt atgtgaagat gagctgaact
      241 cgatttatca gaggcaagtt gtctttatgt tataagtgtt ttaaccttat cggaagtata
      301 atttatatgc cagaaaaata ttcacctgat gatcagagca gtactattga tccatattta
      361 attacttctg ttcgtcagac cttggaagaa caaggtgcag cattacaaaa cttgtctaaa
      421 caactggatt ccgggcagta ccagtgtgtc cttaatttga taatgaactg taaagggcac
      481 gttattcttt cgggaatggg taaatcaggg catgtcggtc gtaaaatgtc agcgacgctg
      541 gcctctaccg gtaccccgag tttctttatt catcctgcag aagcgttcca tggcgatctg
      601 ggtatgatta cgccctgcga tcttttgatc cttatttctg ccagcggtga aacggatgaa
      661 atcctcaagc tagttccttc actgaaaaat ttcggcaacc gaattatcgc cattaccaat
      721 aatggaaatt ccacgctggc gaaaaatgct gatgccgtgc tggaactcca tatggcgaat
      781 gaaacctgcc cgaataatct tgcaccaaca acgtctacca ccctgaccat ggcgatcggc
      841 gatgcgctgg cgattgccat gatccaccaa cgcaaattta tgccgaatga ttttgcgcgc
      901 tatcacccgg gcggttcatt aggtcgtcgc ctgctgaccc gcgttgctga tgtcatgcag
      961 catgatgttc ctgcggtaca gctggatgcg tcatttaaaa ccgtgattca acgtatcacc
     1021 agcggatgcc agggaatggt gatggtagaa gacgcagaag gtgggctggc gggcattata
     1081 accgacggtg acctgcgtcg ctttatggaa aaagaggatt ctctgacatc cgctacggct
     1141 gcgcagatga tgacacgtga accgctgacg ctaccggaag acaccatgat cattgaagcg
     1201 gaagaaaaaa tgcaaaagca ccgcgtctca acgttattgg tgaccaacaa ggcaaataaa
     1261 gtcactggcc ttgtgcgcat tttcgactaa ttaagcaacg gggtttgaga ggttagtctc
     1321 tcattgagtt tgtgccccac tggaactttg ataatcgtta catgttgata aaagtgaagt
     1381 ctgccgtatc ctggatgcgt gctcatctgt ctgccatctc actgtcagat atccaaaaac
     1441 acctggtgaa aatcatcatt ctggcaccga tggcggtgct gctgatctat ctggctatct
     1501 tcagccagcc tcgctatatg agcgagtcga aagtcgccat taaacgctcg gatgatttaa
     1561 acagcggcag cctgaatttt ggtctgcttc tgggtgcctc taaccccagt tccgcagaag
     1621 atgcgttgta tctgaaagag tacatcaact cgccggatat gctggcggcg ctggataaac
     1681 aactaaattt tcgtgaagcg ttcagccaca gcgggctcga ttttcttaat catcttagca
     1741 aggatgaaac cgcagaaggc ttcctgaagt actacaagga ccgtatcaac gtctcgtatg
     1801 acgataaaac cggattactg aatattcaga cgcagggctt tagcccggag tttgcgctta
     1861 agtttaacca gaccgtgctg aaagagtcag agcgctttat caatgagatg tcacatcgca
     1921 tcgcgcgtga ccagcttgcc tttgcagaaa cggagatgga aaaggcacgc cagcgtctgg
     1981 acgccagcaa agcggaattg ctctcttatc aggacaacaa caacgttctg gatccacagg
     2041 cacaggcaca ggcggcgagc acgttagtca atacgctgat gggccagaag atccagatgg
     2101 aagcggatct gcgtaacttg ctgacgtatc tgcgtgagga cgccccgcaa gtggtgagtg
     2161 cgcgtaatgc gattcagtca ttacaggcac aaatcgatga agaaaaaagc aaaatcactg
     2221 caccacaggg tgacaagcta aaccgtatgg cggtggattt tgaagaaatc aaatcaaaag
     2281 ttgagttcaa caccgagctc tacaaactga ccctgacctc cattgaaaag acccgtgtag
     2341 aagcagctcg taagctcaag gtgctgtcag tgatcagttc gccacagttg ccgcaggaat
     2401 cgtcttttcc aaatatccct tatttgatcg cctgctggtt actggtgtgc tgcctgctgt
     2461 tcggcaccct gaaactgttg ctggctgtca ttgaagatca ccgagactaa cgctgtcgct
     2521 gaatgagttt gtgatgaaat tatttaaatc aattttactg attgccgcct gtcacgcggc
     2581 gcaggccagc gcggccattg atattagcgc tgacccaaac ctgacaggag ccgcgccgct
     2641 taccggtatt ctgaacggac agaagtcgga tacgcaaaac atgagcggct tcgacaatac
     2701 cccaccgccc gcaccgccgg tggtaatgag ccgtatgttt ggtgctcaac ttttcaacgg
     2761 caccagcgcg gatagcggtg cgacggtagg attcaaccct gactatattc tgaatccggg
     2821 tgatagcatt caggttcgct tgtggggtgc gttcaccttt gatggtgcgt tacaggttga
     2881 tcccaaaggt aatattttcc tgccgaacgt tggtccggtg aaagttgctg gcgtcagtaa
     2941 tagtcagcta aatgccctgg tcacatccaa agtgaaggaa gtataccagt ccaacgtcaa
     3001 cgtctacgcc tccttattac aggcgcagcc agtaaaagtg tacgtgaccg gatttgtgcg
     3061 taatcctggt ctgtatggcg gtgtgacgtc tgattcgtta ctcaattatc tgatcaaggc
     3121 tggcggcgtt gatccagagc gcggaagtta cgttgatatt gtggtcaagc gcggtaaccg
     3181 cgtgcgctcc aacgtcaacc tgtacgactt cctgctgaac ggcaaactgg ggctttcgca
     3241 gttcgccgat ggtgacacca tcatcgtcgg gccgcgtcag catactttca gcgttcaggg
     3301 cgatgtcttt aacagctacg actttgagtt ccgcgaaagc agcattcccg taacggaagc
     3361 tttaagctgg gcgcgcccta agcctggcgc gactcacatt acgattatgc gtaaacaggg
     3421 gctgcaaaaa cgcagcgaat actatccgat cagttctgcg ccaggtcgta tgttgcaaaa
     3481 tggcgatacc ttaatcgtga gcactgaccg ctatgccggt accattcagg ttcgggttga
     3541 aggcgcgcac tccggtgaac atgccatggt actgccttat ggttccacta tgcgtgcggt
     3601 tctggaaaaa gtccgcccga acagcatgtc gcagatgaac gcggttcagc tttatcgccc
     3661 atcagtggct cagcgtcaga aagagatgct gaatctctcg ctgcaaaaac tggaggaagc
     3721 atcactttct gcccagtcct ccaccaaaga agaagccagc ctgcgaatgc aggaagcaca
     3781 actgatcagc cgctttgtgg cgaaagcacg caccgtggtt ccgaaaggtg aagtgatcct
     3841 caacgaatcc aatattgatt ctgttctgct tgaagatggc gacgtcatca atattccgga
     3901 gaaaacatcg ctggttatgg ttcatggcga agtgctgttc ccgaacgcgg tgagctggca
     3961 gaagggtatg accaccgagg attacatcga gaaatgtggt ggcctgacgc aaaaatcggg
     4021 taacgccaga attatcgtca ttcgtcagaa cggtgctgcc gtcaacgcgg aagacgtgga
     4081 ttcactcaag ccgggcgatg agattatggt tctgccgaaa tatgaatcga aaaatattga
     4141 agttacccgt ggtatttcca caatcctcta tcagctggcg gtgggtgcaa aagtgattct
     4201 gtctttgtaa ggagttgaga tgagcaaagc agttattgtc atcccggctc gctatggctc
     4261 ctcgcgcctg ccgggtaagc cactgctcga tattgttggt aaaccgatga tccagcatgt
     4321 ttacgagcgt gcgttacagg tggcgggcgt cgcagaagtt tgggtggcaa cagacgatca
     4381 gcgtgttgaa aaggccgtgc aggcatttgg cgggaaagcc atcatgacgc gcaacgatca
     4441 tgaatccggc accgatcggc tggtcgaggt gatgcataaa gtcgaggcag atatctacat
     4501 taacctgcag ggcgacgaac caatgattcg cccgcgggat gtagaaacgc tgttacaagg
     4561 aatgcgtgat gaccccgcgt tgccagtggc aacgctatgc cacgcgattt ctgccgaaga
     4621 agcggctgag ccaagcacgg taaaagtggt ggtaaatacc caccaggatg cgctttattt
     4681 cagccgttcg ccgattccgt atccgcgtaa tgctgaaaaa gcgcgctacc tgaaacacgt
     4741 tggtatttac gcttatcgtc gcgatgtgct gcaaaactac agccagttac cggagtccat
     4801 gccagagcag gcagaatcac tggagcagct gcggttaatg agcgcgggga tcaacatccg
     4861 cacatttgag gttgccgcaa ccggtccggg cgtcgacacc ccagcatgcc tggaaaaagt
     4921 gcgcgccctg atggcacagg aactggctga aaacgcatga ttggcatttt ctcgtccggt
     4981 atctggcgta ttccgcatct ggagaaattt ctggcgcaac cgtgccagaa actttctctg
     5041 ctgcgccctg ttccgcaaga agttgatgct atcgccgtgt ggggacatcg tcccagcgcg
     5101 gcaaaaccgg tcgccatcgc caaagcagcg ggaaaacccg tcatccgtct ggaagatgga
     5161 tttgtgcgtt cgctggatct tagcgtcaat ggtgcgccgc cgctttctct cgtggtggat
     5221 gattgtggca tttactacga tgccagcaag ccttcagcac tggagaaact ggtacaggat
     5281 aaagccggaa atacagctct gataagtaag gccagagagg cgatgcacac catcgtgacc
     5341 ggggatttgt cgaaatataa cctggcgcct gcgtttgtgg ctgatgagtc agaatgttca
     5401 gacatcgttc tggttgtcga tcagacattt aatgatatgt cagtgacgta tggtaatgct
     5461 ggtcagaatg agttttctgc catgctggag gccgcgatgg cggaaaatcc tcaagcgaca
     5521 atttgggtga aggtgcaccc agatgtactg aaaggaaaga aaacaggtta tttcgccgat
     5581 ctgcgcgcca cgcaacgagt acgtttgatt acggagaata tcagtccgca gtcgctgttg
     5641 cgacatattt ccagagttta cgtcgtgacc tcccaatacg gctttgaggc cttactggca
     5701 ggaaaaccag tgacatgctt cggtcaaccc tggtatgcag gctggggctt aacggacgat
     5761 cgtcatccgc agtcggcttt gttatctgcc cgacgcggtt ctgtcacgct ggaagaactt
     5821 tttgccgcag catacctgcg ttactgtcgc tatatcgacc cactaacggg ggcagcaagt
     5881 gacctgttta ccgtactgca atggctgcaa ttactgcgca accatcagca acagcgtaat
     5941 ggctatttat gggcgccagg cttaacgctg tggaagtcag caatcctgaa acctttcttg
     6001 cgtaccgcga caaacaggct gagttattca cgtcgctgta ctgctgccac cgcctgcgtg
     6061 gtatggggtg taaaaggaga acagcaatgg caggtcgaag cgcagcgaaa atcactgcca
     6121 ttatggcgaa tggaagatgg ttttctgcgt tcatccggac ttggctcgga cctgctgcca
     6181 tcgctatcgc tggtgctgga taaacgcgga atttactatg acgccacgcg ccccagcgac
     6241 ctggaagtgc tgcttaatca cagccagtta acgctggcgc agcagatgcg agctgagaag
     6301 ttgcggcaac gactggttga aagcaaactg agtaagtaca acctgggagc cgatttctct
     6361 ctgcctgccg aagccaaagg taaaaaagtt atcctggtgc cgggtcaggt agaggacgat
     6421 tcctctatta aaacaggcac tatctcaatt aagagcaacc ttgagttatt acgcacagta
     6481 cgcgagcgta atccgcacgc ctacattatt tataaaccgc acccggatgt actggtgggg
     6541 aatcgcaagg gcgatattcc ggcagaactg attgctgaac ttgctgatta tcaggcactg
     6601 gacgcagata ttattcaatg cattcagcac gcagatgaag tgcataccat gacgtcgctg
     6661 tcggggtttg aagcgttatt acatggtaag cacgtacatt gttacggcct gcccttctat
     6721 gctggttggg gtttaacctt cgatgaacat cactgcgggc gtcgtgagcg agaattaacg
     6781 ttagcggatt tgctatatca ggcgctgatt gtttatccaa cttatatcca cccaacacgg
     6841 ctacaaccta ttactgttga agaggcagcg gaatatttga tccagacgtc gcgcaagccg
     6901 atgtttatta cccgaaaaaa agcgggatgg gtaatacgct attaccgcaa attaattatg
     6961 ttctgcaagg ttagatttgg ctaaacaatt tcatagttga ctattacgca aattatttat
     7021 gcaaggtaat gcactaaccg ttttattatc cggtaaaaaa tatctgctat tgcagggacc
     7081 gatgggaccc tttttcagtg atgtagccga gtggctggag tcattaggcc gtaacgctgt
     7141 gaatgttgta ttcaatggag gggattgttt ttactgtcgc caccgacatt atcaggctta
     7201 ctaccagaca ccgaaagagt ttcccggttg gttacgggat ctccaccggc aatatgactt
     7261 tgataccatc ctctgctttg gtgactgccg cccattacac aaggaagcaa aatattgggc
     7321 aaagtcgaaa ggtatccgct ttctggcatt tgaggaaggg tatttacgcc cgcagtttat
     7381 taccgttgaa gaaggcggag ttaacgcata ttcatcgcta ccgcgcgatc cggattttta
     7441 tcgtaagtta ccagatatgc ctgcgccgca cgttgagaac ttaaaacctt caacgatgaa
     7501 acgtataggt catgcgatgt ggtattacct gatgggctgg cattaccgcc atgagttccc
     7561 tcgctaccgc caccataaat cgttttcccc ctggtatgaa gcacgttgct gggttcgttc
     7621 atactggcgc aagcaacttt acaaggtaac acagcgtaag gtattgccga ggttaatgaa
     7681 cgagttggac caactttatt atcttgccgt tttgcaggta tataacgata gccagattcg
     7741 taaccacagt aattataacg atgtgcgtga ctatattaat gaagtcatgt actcattttc
     7801 acgtaaagcg ccgaaagaaa gttatttggt gatcaaacat catccgatgg atcgtggtca
     7861 cagactctat cgaccattaa ttaagcggtt gagtaaggaa tatggcttag gtgggagagt
     7921 catttatgtg cacgatctcc cgatgccggg actattacgc catgcaaaag cggtggtgac
     7981 aattaacagt acggtgggaa tctctgcact gattcataac aaaccactta aagtgatggg
     8041 caatgccctc tacgacatca agggcttgac atatcaaggg catttacacc agttctggca
     8101 ggctgatttt aaaccagata tgaaactgtt taagaagttt cgtggatata tattgatgaa
     8161 gacacagctt aatgcagttt a
//
LOCUS       DAZGHF010000180         8008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGHF010000180 DAZGHF010000000
VERSION     DAZGHF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.181"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..4308
                     /locus_tag="ACHK3A_004464"
     CDS             <1..4308
                     /locus_tag="ACHK3A_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO8742459.1"
                     /translation="DEVRSKPYWSTDVEMAARAFERYVQDKARMAGVENDYLVNIRKA
                     PEHNTDNTWAYPTNAELDGGIRDAFDHLFSTLKTRETDKGVAFYSRKGVTRTPEGNLI
                     SDVNRSAAEAKGSPVPQVEAVARGVMSGIKDSDLKVRVVKSQKEAEALAGESFDGYGR
                     VHAFYRPDKREIVLVADNIPDGRTVREKIRHEIIHHAMEHVVTPAEYQTIIKTVLKTR
                     DSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKFVAAWERVVALVKGVLR
                     RTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGAGASSQYSRSGKPDPFKADAY
                     DAKQFASEVRRIAGLQSVPRGGEVRVGDTPPVLRALGAPNKELVMPAHVVHKATRPEL
                     REHYVSVETMADLPQLLADPVAVMTSKTEHDSLVTLIEAKDRRGAPVIVAIHMRAKGA
                     VAEVNKIASVYGKDNRIALQEQLDTSLLYLNKEKAADWLLSAGLQLPGANTINGSSGF
                     KVLHPEDIRKGAYYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQENIRKTGGKIDDS
                     NNAYMAEELFHGKAENDLNVMKERYVQPLAKLLANYKISQADLDEYLYARHAPERNAH
                     IAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVDDMLARRRELIREAG
                     LEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRESRQAMGRASRAQSP
                     STQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDTMRTIAERKDQE
                     TGETRREVVERPVPMAMMADRYFTTKKNGKTYYIKLHDPRLMRAMKNMGPETSNAVIR
                     TLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKGLDNLSALAV
                     VKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMDR
                     LVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHARDAGLSRQQAASLAK
                     NMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGEGPLLERLRWKNLNVP
                     QKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNLVIMKSVFGGKAGEY
                     WSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGAFSPIGSETSETLSGA
                     LLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRSTPEAYKAFASW
                     LNAFSGGSQYRPGAVDVTPESLKYWIDYISGGTGRFISKTTDAAVKSLNGIDIPEQQV
                     PFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTAFIDENNGKLSMNGLM
                     QDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKIAVDRFNREYNKKVGVE"
     gene            4668..5912
                     /locus_tag="ACHK3A_004465"
     CDS             4668..5912
                     /locus_tag="ACHK3A_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFO8742460.1"
                     /translation="MSKKFTKTLLSSSLVVGLLSVSSFTSASEYSYTALDIVGRHGTT
                     YTIVYNSDSDTAYVKWKTVPAGAEGIESGSIVQGLSKINRDGTVEVTTKDGTSTYRVY
                     DREFHDLMNSLVKQKREGSLGKNEGTKTELVSDADIRNLFGRIAAISEMETNVGLDEY
                     GRVATSAESIKERVSLQSYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIA
                     KDVEEQKEGLAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNADGTLTSADGA
                     TKTIAVNDGLVALSGRTDRIDATVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQ
                     IRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTA
                     AKAGVAASDGDVSYNVGVNFEF"
     gene            5999..6472
                     /locus_tag="ACHK3A_004466"
     CDS             5999..6472
                     /locus_tag="ACHK3A_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742461.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            6843..>8008
                     /locus_tag="ACHK3A_004467"
     CDS             6843..>8008
                     /locus_tag="ACHK3A_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO8742462.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDSSGTVVPAVAGGDIVWNYSKGSGE
                     GTLSQDGKVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTV"
BASE COUNT         2064 a         1692 c         2393 g         1859 t
ORIGIN      
        1 gatgaggtgc gctcaaaacc gtactggtca acggatgttg aaatggcggc gcgtgccttt
       61 gagcgttatg ttcaggataa ggcgcgtatg gctggcgtgg agaatgatta tctggtcaat
      121 atccgtaagg cacctgagca caacacagat aacacctggg cttatccgac gaatgcggaa
      181 ctggatggcg gtattcgtga tgcattcgat cacctgttca gtacgctgaa aacccgtgag
      241 acggacaagg gcgttgcgtt ttattcccgt aagggcgtaa cccgcacacc tgaaggcaat
      301 ctcatttcgg atgttaaccg tagtgcggcg gaagccaaag gcagcccggt cccgcaggtt
      361 gaagcggttg cccgtggcgt gatgagcggc attaaggaca gtgacctgaa ggtccgtgtg
      421 gtgaagtcac agaaagaggc tgaagcgctg gcgggtgaat cgttcgacgg ttacggcagg
      481 gtgcacgcgt tctatcgtcc ggataaacgt gaaattgtcc tggtggcgga taacatccct
      541 gacgggcgga ccgtacgcga gaagatacgt cacgagataa ttcaccatgc catggagcat
      601 gttgtcacgc cagcggaata tcagacgatt atcaaaaccg tgctgaaaac ccgcgacagt
      661 gataacgcca ccatccgtga agcctggcgt aaggttgatg cgtcctatgg taaggaatca
      721 ccggaagtac aggcgggtga atttctggca catatggcgg agaaacagcc gaataaattc
      781 gtggcggcat gggagcgtgt tgttgccctg gtcaaagggg tactgcgtcg tacggggtta
      841 ctgaagccga cggaactgaa cgatatcaga cttgttcgcg agaccatccg tacgttaggc
      901 cagcgtgtgc gggaaggtta cacgccgcgt gaggatggcg cgggcgcatc gtctcagtac
      961 tcccgtagtg gtaagccgga tccgtttaag gctgacgcct atgatgcaaa gcagtttgct
     1021 tcagaagtgc gtcgcattgc cggattacaa tcggttccac gaggtggaga ggtgcgggtt
     1081 ggtgacactc cgccagtgtt aagagcactt ggcgcaccaa ataaagagct ggttatgcca
     1141 gctcatgtgg ttcacaaggc caccagacca gaattacgtg agcactatgt ttctgttgaa
     1201 acgatggctg acttaccgca attgcttgcc gacccggttg cggtgatgac atcaaagacg
     1261 gaacatgact cactggtaac tttgattgag gctaaggaca gaagaggtgc acctgtcatt
     1321 gttgcaattc acatgcgtgc taaaggggcg gtagctgagg ttaataagat tgccagtgtc
     1381 tacggaaagg acaacaggat cgctttgcag gaacagttgg atacttcctt gctgtatctg
     1441 aataaagaaa aagccgctga ctggcttctt tcagccgggc tccaattgcc cggggcgaac
     1501 accatcaacg gctcttctgg atttaaagta ctacatcctg aagatattcg caagggggcg
     1561 tattactccc gcaccagcag tctgacaccg gaagagacca ttgcatcgcg ttttgttcgc
     1621 cagatgcagg ataaattcca ggtgctgaag gcggtgcagg agaatatccg taaaactggc
     1681 ggaaaaatag atgacagcaa taacgcctat atggctgaag aactcttcca cgggaaggcg
     1741 gaaaacgacc tgaacgtaat gaaggagcgc tacgttcagc cgctggccaa attgctggcg
     1801 aactacaaaa tctctcaggc cgatctggat gagtacctct acgcccgtca cgcgccggaa
     1861 cgtaacgcac atattgcgaa aatcaaccct aaaatgccgg acggcggttc ggggatgacc
     1921 aacgcggagg cggcggaaat catgcagcgt gtgcgtaaca gcggcaaaca ggcacagtat
     1981 gaccgtctgg cagggattgt tgacgatatg ctggcccgtc gccgtgagct tatccgtgag
     2041 gccggacttg aagagaacgg tgtggtggat gcctggcaga acgcctaccg ttactacgtt
     2101 cccctgaaag gtcaggatgt tgacggtgtg gtgtcacttc cccgtacagg taagggcttc
     2161 accatcggcg gacgtgaaag caggcaggcc atggggcgtg catcccgtgc acagtctccg
     2221 tccactcagg cgatacagga cctgagcgaa tcgctgatcc gccatcgcaa aaacgaagtg
     2281 ggtaacgcct tcctgaaact ggtgcaggat aatcccgaca aggattactg gcaggtattc
     2341 accgatgaca gaccggatac catgcggacg attgcagagc gcaaggacca ggaaactggt
     2401 gaaacccgtc gcgaagttgt cgaacgccct gtaccgatgg caatgatggc agaccgctac
     2461 ttcaccacca aaaagaacgg caaaacgtac tacatcaaac tccatgaccc gcgcctgatg
     2521 cgtgcgatga agaatatggg accggaaacc agtaacgccg taatccgtac gctggggaaa
     2581 gttaaccgct tcctggcaac ggtgaacacg tcgtataacc cggaattcct ggtcagtaac
     2641 ttcatccgtg acgtgcagac ggcggtgatg aacctgaagg ctgagcaggg aaggagcgac
     2701 ggtaaactga aagggctgga taacttatcc gccctggctg tagtcaaaga cagccgttct
     2761 gccatgtcag ccgtatacgc cagtctgcgt ggtaaaaccc tcacgggcaa aggtgcacag
     2821 tggcagaagg tgtggaaaga gtttgttgag gacggaggta aaaccggttg gtttaacatg
     2881 ggtgaccttg aaggccagca gaaggaaatg gatcgccttg tttcgctggc gaagggagga
     2941 tggaaaggcc agagtatcgg tgcatggaat tcgttcctta accttgtcga ggatgccaac
     3001 ggcgcggttg aaaacgcact gcgtctgtca gcttataaac acgcccgtga tgccggtttg
     3061 tcacgccagc aggcggcgtc tcttgccaaa aacatgacgg tgaactttaa ccgtcgtggt
     3121 gagcaggggg cgctgatgaa ctcgctgtac atgttcgcca acgccagtat tcagggaacg
     3181 gcaaatctgg tgagaacgct cggacatctt aatggtgagg gacctttact ggagcgcctt
     3241 cgctggaaga atctgaatgt tccgcagaaa atcgcgcttg ccgctgtggg agcaggttat
     3301 ctgcttggct cgcttaaccg cagtgtggcg ggtgaggatg atgacggggt taactggtat
     3361 gacaaggtgc cgtctcatgt gaaagagcgt aacctcgtca ttatgaaatc ggtgttcggg
     3421 ggcaaggccg gagagtactg gagtattcct ctgccttacg ggtacaacgt tttcttcctg
     3481 ctcgggcata ccgctgaagg tgtggcggcg ggtgacctga cggcgtcccg tgctgccggt
     3541 aatgttgttg gtggtgtgct tggtgcattc agcccgattg gcagtgagac gtcggaaaca
     3601 ctgtccgggg cattgctgaa aaatgcagcg ccgaccattc tgcgtccgtt tgcgaacctt
     3661 gccatgaatg aaaacttcat gggggcgcag atttaccagg agaacatgcc gtttggtaca
     3721 ccaaaacctg acagccagct gggaagacgt tcaacgccag aagcgtacaa ggcgtttgca
     3781 tcctggctga atgcgttctc aggtggcagc cagtaccgtc ccggcgcggt ggatgtcacg
     3841 ccggaatcac tgaaatactg gattgactat atctctggtg gtacagggcg cttcatttcc
     3901 aaaaccacgg atgcggcggt gaaatcgctg aatggtattg atataccgga gcagcaggtg
     3961 cctttcctgg ggaaaatttc aggtgaggtg atgccgtatg ctgaccagca gaagatgtac
     4021 gaccggatga cagaggttgc gcagtaccac gcagagctga agagtctgac cggtgcagaa
     4081 agaacggcgt tcattgacga gaacaacgga aaattgtcga tgaacgggct tatgcaggat
     4141 acccggaaga gactgaagga tttgcgtaaa cagcgtgatg ccatttacgc cgacagtact
     4201 ctcagtctgg cgcaacagtc ggcgatggtg aaatcggtag agcgggatat gaaaattgcc
     4261 gtggatcggt ttaaccgcga gtacaacaaa aaagtgggag tggagtaaag aacatgctcc
     4321 gtacggaagt gcggggctga tataaagtca cgcagggaaa tattgtagat gaactgaaca
     4381 gttaaattcg gataatgttg tgatattgat cagaaattac agtgtaagca gatgtgaaac
     4441 agaatgcctg atgagagtaa gttttttctg atgcctgttc cggtggtgta gtcgtggttc
     4501 tgagtgacta caccaagaat aatgtaagac aaatcctaaa acattaaatt aatattttgg
     4561 ggttattttc ggaatatcct gaataaagcc gcaattaatc atatttattt atgagtatga
     4621 ggtgtagtgg ctgatttgta tacagataat ttaacaggat atccataatg agtaaaaaat
     4681 ttacaaagac acttctgtca tcttctctgg ttgtaggtct gttgagtgtt agttctttta
     4741 caagtgcaag tgaatatagc tacactgcct tggatattgt cggtagacac ggaactactt
     4801 acaccattgt ttataatagt gattctgaca ccgcttatgt taaatggaaa acagtacctg
     4861 ccggtgctga agggattgaa agtggaagta ttgtacaagg attatcaaaa attaatagag
     4921 atgggactgt agaagttact accaaggatg gcactagtac atatcgtgtg tatgaccggg
     4981 agtttcatga cctaatgaat tcactggtca agcagaaaag agaaggttct cttggaaaga
     5041 acgagggtac taaaacagag cttgtatctg atgctgatat tcggaatctt tttggcagaa
     5101 ttgctgcgat ctctgaaatg gaaactaatg ttggtctgga tgaatatggg cgagtggcaa
     5161 ctagtgcaga gagtattaaa gaacgcgtgt ctttgcagag ttatctggct acagaggctg
     5221 caaatagcgc aattggccta gataaaaacg gtaagctaac tctgaatgag acagatattg
     5281 taacgccgtt acgtatcaat gacggcgttg tcactatagc gaaagatgtg gaagaacaga
     5341 aagaagggct ggcgatccag aagaaaggac tgtctgagct ttcatcccgc acagatatca
     5401 ttgatgcagc aacggctgcg aatacgaata aaattgattc ccgaaccacc gttggtgtga
     5461 atgcggacgg aaccttgacc agcgctgatg gcgctacaaa aacgattgct gttaatgacg
     5521 gtctcgttgc tctgtccggc agaacagacc gtattgatgc tactgttggt agcgttgacc
     5581 gccgggttac taaaaataca caggctattc agtccaatac tcgccagttg caggaacata
     5641 atgcacgcct gaacagccag cagcgtcaaa ttcgtgagaa tcacgaagag atgaagcgtg
     5701 cagcagcaca gagtgcagcg ctggcggggc ttttccagcc gtacagtgtg gggaaattca
     5761 acgccactgc tgccctgggg gggtacagtg ataaacaggc tgttgccgtg ggtgttggtt
     5821 accgtttcaa tgagcagaca gcagcgaaag caggtgtggc agcgagtgat ggcgatgtgt
     5881 cctacaacgt aggtgtgaac tttgagtttt aattgctgac agtgacatga agcatgacgg
     5941 caggcgtgga gttttgcctg ccgttttttc tcatgtgagc atgaaacagg tgacagcgat
     6001 gaaagtgatt ctggcgaccc gcaaccgtta tctggagtat ggcctgcagc agatgcttga
     6061 ggggtacagg ataatactgg cgagggagtt ttttacacca gagaacagga agagtgtacc
     6121 ggcacatgat gagtcatggg tgataatctg tgatgccctc cttggcaggc tgatgtgctg
     6181 catgtttcag ggacgccgtt atcttcagat tgatgcggaa gatgttacgg ggcgcctgga
     6241 gacgtacagg aaaatccgga atggtgagtg ggtacataat acgtatgcgc gacctctgac
     6301 gatgtccgag atggtggtga tgtttggata cgtgtaccgg gagtcaaagc cctgccatct
     6361 tgcccgtgaa atggggataa acacgaagac ggtgaacacc tttctgtata tgggacttgg
     6421 taaaaatggt cttaagtaca ggagtgtgaa gcacctggtt ggacgtgcgt agccggaatg
     6481 catattcgtg agtcgggtaa tgagagtgga acggtggcag tgagaatgga acagaaaaaa
     6541 atcagatgcg gaaagcggaa atacacagcg ggtgcgatgt gcttcatgct gtttgtatgg
     6601 ctggggggtg tggtgacatt ctgtgtgctg gccgctctga taatgaggtg aaaacggaag
     6661 agagtttact tccttaattc gaactttctg ataactgaga acttgttaaa tgcctgtggg
     6721 tttatgtatt taaatcaatt ggttaatggt gattttatct ttatcagtca aagttcgttt
     6781 tagtatgatg attgtgcccg ctgtttattg ttgtccggga gaaaatacag ggaatccagg
     6841 ggatgaacaa aatatactca ctcagagtta atgacagaaa agaactggtt gctgtaccgg
     6901 aaacggtggg gggcaggaaa aagtcgtccg gcattcccgg tgcggggatg ctttcccgtc
     6961 ttcttctggc ctcaggagcc gttgccggcg ttctgttttc ttctcctgcg ctggcatccg
     7021 ttgtcgggaa tactctgcca tggcagacct acagggattt tgctgaaaac aaaggggcgt
     7081 ttcatgccgg ggccacaaat attcctcttt atggtaagaa tggggctgtc agtgggcggc
     7141 tggacaaggc tccgatgatg gatttcagtg ttgtggacca gatactgggg gtggcaacac
     7201 tgatttctcc gcaatatgtg gcgggggtta agcataacgg cagctacaat acggtgcgtt
     7261 ttggttatgc ggacgatacg acatatcgcc ttgttgaccg taatgagcac tggcgtgatt
     7321 ttcatactcc ccggctgaac aaactggtga cagaagttgc cccggtgagc gtcacggatg
     7381 ccggaaccgg caagggggta taccagaacc gcagtcgtta tccggtgttt tatcgtatgg
     7441 ggagtggaac gcagtacacc ggagctgctt ccggtgcgct gaccagaata gcggatgctt
     7501 acgtctggag gactggcggg acggtgggaa gtccggtcat ttctgactgg agtctggtgt
     7561 caaacccggg aaatctttac cagggtgtta atggtccgct ggcgtcttac gggacatcag
     7621 gagacagtgg ctcaccgctt tttgcctggg atgcggtgaa gaagcagtgg gtatttgttg
     7681 ctgtacttca tggttatgcc ggtgagaacg ggaagacgaa ctggtttacg gtgattcctg
     7741 caggggatgt gaataacacg ataaagcagg acagttcagg caccgtcgtg ccggcagttg
     7801 ccggtggtga cattgtgtgg aattacagta agggaagcgg agagggaacc ctttctcagg
     7861 atgggaaggt atggaagatg aacggcttca ggggagggag tctgaatgac gggaaagata
     7921 tcacctttgg gggtaagggc acggttgttc tgaaggatga tgttgtccag ggggcgggtt
     7981 cgctcacgtt taatggtgat tatacggt
//
LOCUS       DAZGHF010000181         7983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGHF010000181 DAZGHF010000000
VERSION     DAZGHF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.182"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(92..742)
                     /gene="zinT"
                     /locus_tag="ACHK3A_004468"
     CDS             complement(92..742)
                     /gene="zinT"
                     /locus_tag="ACHK3A_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO8742463.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(999..1634)
                     /gene="msrQ"
                     /locus_tag="ACHK3A_004469"
     CDS             complement(999..1634)
                     /gene="msrQ"
                     /locus_tag="ACHK3A_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO8742464.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSL"
     gene            complement(1635..2639)
                     /gene="msrP"
                     /locus_tag="ACHK3A_004470"
     CDS             complement(1635..2639)
                     /gene="msrP"
                     /locus_tag="ACHK3A_004470"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO8742465.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTHERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(2748..3161)
                     /gene="hiuH"
                     /locus_tag="ACHK3A_004471"
     CDS             complement(2748..3161)
                     /gene="hiuH"
                     /locus_tag="ACHK3A_004471"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO8742466.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3294..3965
                     /gene="hprR"
                     /locus_tag="ACHK3A_004472"
     CDS             3294..3965
                     /gene="hprR"
                     /locus_tag="ACHK3A_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO8742467.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3965..5323
                     /gene="hprS"
                     /locus_tag="ACHK3A_004473"
     CDS             3965..5323
                     /gene="hprS"
                     /locus_tag="ACHK3A_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO8742468.1"
                     /translation="MKKLSITVRLTLLFILVLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGHGINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDNREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICL
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIQITSFLDTNGSLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(5430..6281)
                     /gene="hchA"
                     /locus_tag="ACHK3A_004474"
     CDS             complement(5430..6281)
                     /gene="hchA"
                     /locus_tag="ACHK3A_004474"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO8742469.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(6873..7946)
                     /locus_tag="ACHK3A_004475"
     CDS             complement(6873..7946)
                     /locus_tag="ACHK3A_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO8742470.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRDVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
BASE COUNT         2213 a         1674 c         1827 g         2269 t
ORIGIN      
        1 tctgagtttg tcttacttat ttgtcttggt aaattattgt tgttactacc aggcactgtt
       61 ctgttaaata actgaagtca gcgcaaaaaa atcaatgaga catcatctcc tcgaccactt
      121 cttcgctact caactgatag ggataatacg ttggccagtt ttccatttca ttcaataaag
      181 attgctggga atcattaccc ataaaaatgt gaaaatgaga tgattttcgt ggtgcaatta
      241 tatgatcgct aaattgtata tatttagggg ctttgctttc aggatcttta cattcgaata
      301 agtaacgaac gcctttcttg cctgatttat aggtgagtat tttgtatcca tcgtaatcat
      361 atttacagga tgttgtttca ttatttctat ggaattcaac aatgccgtcc tcaatgccaa
      421 tcatctcgat atctgttgca taacctttgt gataataatc tttaatttca gcaaatgttt
      481 tagttttatc tgcatccgct ttcttctgaa agacggggtc aagtttgcca ctttgcaata
      541 aaggataaac ggattgccag actccatccc agtcacttag cgttcggttt tgtacattgg
      601 catcatcaaa aacaccatta gcagcttttt gttcgacctc tgttaagggt ttgccgtgtg
      661 agtgatgacc atgcgaaaag gcaggagcgc taacaataaa gacacctaaa gcaacagcca
      721 gtttatgaag acgaatcgcc aaaacagttt cctccagagt taatgtatat gatgtgtaat
      781 gttatattgt aacatataac aaaatgcaac aactcttagg agacaagaga atagttctct
      841 caggtgtgct tatctatctt attgaaataa aataaattat gtcgatgatt tgaggttgat
      901 gacaaacgaa acataaccta atgcgatctg tgtatgccgg ataaggcgct cgcgccgcat
      961 ccggcaggga taacgcgtac tttgtcggca atatcaagtt atagagataa tttgttgtga
     1021 acctgcttgc gtaaccggtt gaaaagggaa agcaacttct tataccgtaa ggctaaaagc
     1081 agtaccgcca gcccagcgta gatgagtggc tgcggtgaaa taatcttcac tgaccacaga
     1141 taatgtattg gggccaggat cgcgacaaga tagacgaaat tatgcaactg ttgccagtgc
     1201 ttgccgagtt ttcgctgcat cgcctgggtt gaagtaaacg ctaaagcaag caaaataacc
     1261 cagctgataa ttcctaacgt taaataaggc cgggtaatta actccttacc taataacgcc
     1321 agattattca cgcccagctc cagtaatgcg taactggtta aatgcagtgt cgcccaggca
     1381 aagcaccata agcctaacag gcggcgagtg cgtatcaata acggctgttt tgcgtagcgt
     1441 gccagaggtg tgattaacaa ggccgcaagc aaaaatttca gtgcagtgcg accggtaaaa
     1501 tgctgaatat ctttcaccgg atcggcaccc aatccaccgt gattaatcgc ccagaccagc
     1561 cagagaaacg gcaacaatcc ggcaagatgc aggcaaactt tcagccatgt cacctgtttt
     1621 gctgtcagac gcatttagaa attctcccgc aaatccaggc cacgatacag cgatgccacc
     1681 tgctcggcgt aaccattaaa cagtagcgtt ggctggcgct gtacatcgag gatgccgcct
     1741 gaaccaataa atcgttcggt agcctgtgac cagcgcgggt gatcaacatg cggattaacg
     1801 ttggcgtaaa aaccgtattc gtcaggcgct gccagattcc aggtggttgg cggacgctcg
     1861 tgggtcagct taatactgac gatcgattta atccctttaa agccatattt ccacggcaca
     1921 atcagtcgca ccggcgcgcc attttgtggc ggtaacgcct tgccataaac acctacggtc
     1981 atcagtgtga gcggatgcat tgcttcgtcg agacgcaatc cttcgacata aggatatttc
     2041 agcccgccgc cgataaagcg gtcctgctgg cctggcatct gttccggtgc ataaattgtt
     2101 tcgaaagcga catacttcgc attgctggtg ggttcggcaa gcgccagcaa tttgtgcagc
     2161 ggaaaaccaa tccacggcac caccatcgac catgcttcca cgcagcgcat acgataaata
     2221 cgctcttcca gcgggaagcg acgggttaaa tcatcgtgat cgagggttaa tggttttgct
     2281 acttcgccgc tgattttcag tgtccatgga tcggttttca ggctaccagc attagcggcg
     2341 ggatcggctt tatccagccc gaattcatag aagttgttat aaccggaaac tttatctgct
     2401 ggcgtcagtg gcaggttatt ttgccaggcg gcaggcttgc tgaactccag cggttttccg
     2461 gcgggggcgg gtggacgatc gttcccttta aaccagctaa gcagatcggc atgcgcagcg
     2521 tgaggcaaag atagtgcagc cgcgctgatg cccagtgctt ttaacacctg ccgacgcttc
     2581 ataaagaata ccgactcggc cgtaacatct gattctttta aaaattgatt ctttttcata
     2641 agccatcaca cttttttgtc gttagccagc aagagccgtt tactgttaat ctgtcaggga
     2701 gtcacctccc taacagtagc agagatctgc ttttatatat ttatgcatta actgccacga
     2761 taggttgaat acccatattg gctaagtaat aaaggcacat gataatgctc gttcacttta
     2821 ttaatatgaa attcaacggg gatctcaggg aagaaacttt caagattttg tttcttgaaa
     2881 tagtccccgg ttttaaatac gacacggtaa tcgcccgtag tcgcagtttg ctcgggccac
     2941 agtgccttaa ttcgtccatc cttatctgtt ttggcggtat taagttgtaa ccagccgtta
     3001 tccgctttct tttcaagagt gactgtcacg tcggcagcgg gttttcctgt ttgctggttc
     3061 aaaatgtgca cgctaagaat gttttgttgt gcagcataga ccaaagaagg taatgaaaat
     3121 gcagccgtta ctacggagag tactaaataa cgctttaaca tgtttatatc cttgtcatgt
     3181 gaatgagttt tgaggatagc gaaaaagcgt ccagggtaac cttacagcag cattacaatt
     3241 ttgtaatgaa ccaggctgtt tctataacat atgatttatg gcatattatt ttcatgaaga
     3301 ttttacttat tgaagataat caaaggaccc aggagtgggt aacgcagggg ctttccgaag
     3361 cgggttatgt cattgatgcc gtttctgatg gcagagatgg gctttatctt gcgctgaagg
     3421 atgattatgc attgatcatt ctggatatta tgcttccggg tatggatggc tggcagatct
     3481 tacaaacgtt aagaacggca aagcaaactc ctgttatttg ccttacagca agggattctg
     3541 tcgatgacag agtcagaggg ctggacagtg gagcaaatga ttatctggta aaaccttttt
     3601 cattttctga gttgctggca agggttcggg cacaattaag gcaacatcac gctttgaatt
     3661 caacattaga aatcagtggc ttaagaatgg actctgttag tcaaagtgtg agcaggaaca
     3721 atatcagtat tacactgacg cgcaaggagt ttcagttact ttggctactg gcctccagag
     3781 ctggcgaaat tatacccaga acggttattg cgagtgaaat ttggggaatc aactttgata
     3841 gtgataccaa tacggtggat gtcgccattc gcaggctccg cgcaaaagtt gatgatcctt
     3901 ttcctgaaaa gctcattgcc acaatccgtg ggatgggata ttcattcgta gcggtaaaaa
     3961 aataatgaaa aaattatcta taaccgtccg tttaaccttg ctttttatat tggtgctgtc
     4021 tgttgctggc gctggaattg tatggactct ctataatggc ctggcaagtg agttgaaatg
     4081 gcgcgatgat acaacactca ttaaccggac agcgcagatc aagcagttgt taattgatgg
     4141 ggtaaatcca gatacgttac ctgtctattt taaccggatg atggatgtta gtcaggatat
     4201 cttgatcatt catggtcatg gcatcaataa aattgttaac cggacaaatg tcagtgatgg
     4261 catgttaaat aacatacctg ctagtgagac aatcagcgca gctggcattt acagaagcat
     4321 tattaatgac acagagatag atgctttacg aattaatatt gatgaagttt cgccatcatt
     4381 aacggttact gtggctaaat tggcttcagc cagacataac atgcttgaac agtataaaat
     4441 taatagcatt ataatttgca ttgtcgccat tgtactttgc tcagtattaa gtccgctgtt
     4501 aatcagaacg gggttacgag agatcaaaaa gttgagtggt gtaacggaag cgctgaatta
     4561 taacgataac cgggagcctg ttgaggttag cgcattaccg agagaactaa aacctcttgg
     4621 gcaggcgttg aataaaatgc atcatgcttt agtcaaagat tttgagcgtc taagtcagtt
     4681 tgctgacgat ctcgctcatg aacttagaac accaattaat gcattactgg gtcagaatca
     4741 ggttacgctc agtcaaacca gaagtatcgc tgaatatcaa aaaacaattg ccggaaacat
     4801 tgaagagctg gagaatattt cgcggttaac agagaacata ctgtttcttg ccagggcaga
     4861 taaaaacaat gttttggtga aactggattc gctttctctc aataaggaag tcgaaaattt
     4921 gttggactat cttgaatacc tttcagacga gaaagagatt tgccttaagg tcgagtgcaa
     4981 tcagcaaatc tttgcggata aaattttact gcaacgaatg ttatcgaatc ttattgttaa
     5041 cgctattcga tattcaccag aaaaatcgcg cattcagata accagttttc ttgataccaa
     5101 cggctctctt aatattgata tcgccagtcc tggaacgaaa attcatgagc ctgaaaaact
     5161 cttccgtaga ttttggcggg gagataattc gcgtcattcc gtaggtcagg gacttggtct
     5221 ttctttagtc aaagcgattg ccgaattgca tgggggaagt gctacgtatc actatctcaa
     5281 taagcataac gtgttccgaa ttacgttacc gcaaagaaat taatataggg cttcagtacg
     5341 ccaaaattaa ccgtgaaaaa acgcagtcac gtcgtgagta ttaacgtggc tgcgattgat
     5401 aatgcgctta cattacaatg taacagggat taacccgcgt aagctgccag catttcctgc
     5461 gccgccagtt tacccaacgc attcgctgcg aaaggactgt cgccggtgag aactttacgg
     5521 tccttatgta ctcgcccggt gatgtcgtca ttaatgatgt tcatgcccat tttcttcagc
     5581 tcttcgccga agtaccaggt gagatgaccc ggcatatagc ctatctctgg cgtttgtttg
     5641 tctgcggcgt ctgggaatgc gcaaatggaa taaccattca gtgggttatc gccgtggcga
     5701 agcgccagaa aagccgccgg gccgtggcaa agggagataa caaaacggtc atttttgatt
     5761 gcccactgta aagcggcagc tacgtcctgg ctttcaggta aaccaataag tgcgccatga
     5821 ccaccaggaa caaagattgc tgcatattcg ctatcagcgt tgaggcttgc gacaacatcc
     5881 gcgagtttct tcggattgcg gaacaacgat ttatgctgct caaagaatgg cattactttt
     5941 tcatctttgt gtggcatagc ccagtattca aacttggtca tcagaccgga aatggtcgcc
     6001 acttcgaatt cgaaacctgc agcatggaga tgatacaacg gcagcaacgt ttcaatcgga
     6061 tggttaccgg tcgagaatag tttcccgtta tcggtcggca aataacgttc gtccgcggcg
     6121 atcaccagaa ttttatgttt accgcgatac ggttttggat agtccacgcc atcaagatca
     6181 gagacgggac tggtatattg gctaagcgaa tattctgaag ggaagaatgc attatcttca
     6241 gcaatatcga cctgcgggtt tttacttgtt tgaacagtca tagtgtattc cttattgttg
     6301 cttagttagg gtagtcacta tatttgcgac tgagctggtc gctattttta accaggctga
     6361 aaaattgagt caatatatcg aaagcggtag ttggcgggca taagtatgta atgatgatgc
     6421 tgcactggtg ttgaatatta ttcacgccaa attttcctta ttgagctacg tcgtcgcaag
     6481 tggcctcaat gattttagac cattatcaga ggtccggggc ggcagggaga gatttagtgc
     6541 tgtggaatat tgtggtgctg atgaatattt atatcgcgga ttggtagaga aaaagctttt
     6601 ttcatgcact atttttgcaa atatttctat atggacggcg gttttgttgc ggattaattc
     6661 ataaagaaat gcatttggat gttggccgga agagacgctg gtagatttta ttgagccagg
     6721 aggggtgggg taaggtgctg tatcgatatg ctttggcaat tgcgtgtgcc gcagatggag
     6781 atgaaatgcc aataaaaaag cctgagttcg atgagggacg aactcaggct ggtgttaagt
     6841 tttacagtaa cacgccgcgg cgcgttggct gattagaact ggtagacgat accaactgca
     6901 aactggtcat cagttgcggc atcggtaatc gctgcatcgt ctttatccag caggttaaat
     6961 ttgtatgctg tgtagacgtt catgttctta ttgaagtagt accaggtacc aacttcgata
     7021 taatttacgc ggtcagcatc aacaccttta tgtccatcac gagcataaag atctttgcct
     7081 ttggaatata cgtagcctaa agacggacgt aaaccgaagt cgaactgata ctgtacaaca
     7141 gcttcaaagt tttgggtttt atttgcaaaa tgatcattag ccaaaacggt catgttgcgg
     7201 gtttcaccgt acatcattgc tgcatagata ttatttgcat catacttggc tgctaatgac
     7261 cacacttcag cgttatcacc gtagccgtca ccagcttgtt cattagtacg atcagatttg
     7321 ctatatgcac ctacaaaacc aaaaccttcg atgttgtagt ctacagacaa cgcatagcca
     7381 tcaccgtttt gtttcgtaac gtcacgattg ctgttcttac cttgatattg cacagcaaaa
     7441 ttcagaccat caaccagacc aaagaaatca gagttacggt aggttgcaac accgttggta
     7501 cgaccgttca tgaagttgtc agcggacgcc caggaatcac caccccattc aactaacata
     7561 tcggtataag ctgcggcatc ataagcaaca ccgtagttac gaccatagtc gaaagatcct
     7621 aactcgttat atttcaaacc tgcgaaggtt aaacgggttt tttcctgatt agagccttcg
     7681 ggcttgctgg cgtcgaggtt gtattcaaac tgaccaaaac cagtcagttc gctggtgatt
     7741 tgggtttcac ctttaacacc gaaacgagca taggaggtat cttcgttttc gctgttatta
     7801 tcatcagtat ttgaccagat gcgttcgcca accatcttgc cgtagaaatc aactttattg
     7861 ccatctttat tataaatttc agccgcattt gctgcgccag caactaataa cgccgggacc
     7921 agcattgcca gaacttttct tttcattatg tattcccttg tgattataat cttcatgaat
     7981 ata
//
LOCUS       DAZGHF010000182         7527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGHF010000182 DAZGHF010000000
VERSION     DAZGHF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.183"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            2..856
                     /gene="gatY"
                     /locus_tag="ACHK3A_004476"
     CDS             2..856
                     /gene="gatY"
                     /locus_tag="ACHK3A_004476"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO8742471.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            885..2147
                     /gene="gatZ"
                     /locus_tag="ACHK3A_004477"
     CDS             885..2147
                     /gene="gatZ"
                     /locus_tag="ACHK3A_004477"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO8742472.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMFDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGMEIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2157..2609
                     /gene="gatA"
                     /locus_tag="ACHK3A_004478"
     CDS             2157..2609
                     /gene="gatA"
                     /locus_tag="ACHK3A_004478"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO8742473.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2640..2924
                     /gene="gatB"
                     /locus_tag="ACHK3A_004479"
     CDS             2640..2924
                     /gene="gatB"
                     /locus_tag="ACHK3A_004479"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO8742474.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            2928..4283
                     /locus_tag="ACHK3A_004480"
     CDS             2928..4283
                     /locus_tag="ACHK3A_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO8742475.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            4331..5371
                     /gene="gatD"
                     /locus_tag="ACHK3A_004481"
     CDS             4331..5371
                     /gene="gatD"
                     /locus_tag="ACHK3A_004481"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO8742476.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDVVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5477..6250
                     /locus_tag="ACHK3A_004482"
     CDS             5477..6250
                     /locus_tag="ACHK3A_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO8742477.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6332..7231)
                     /gene="yegS"
                     /locus_tag="ACHK3A_004483"
     CDS             complement(6332..7231)
                     /gene="yegS"
                     /locus_tag="ACHK3A_004483"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO8742478.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1894 a         1677 c         1967 g         1989 t
ORIGIN      
        1 aatgtacgtg gtatcgacaa agcagatgct gaacaacgca cagcgcggcg gttatgcggt
       61 tccggcattc aatattcaca atctcgaaac gatgcaagtg gtggtagaaa ccgctgccaa
      121 cctgcatgcg ccggtcatca tcgccggaac gcctggcaca tttactcatg ccggtacaga
      181 aaatctgttg gcgctggtca gcgcgatggc gaagcaatat caccatccac tggcaattca
      241 tctcgaccat cacacgaaat ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc
      301 agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat atttcacggg tcaaagaggt
      361 ggtggatttt tgccatcgct ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg
      421 ccaggaagat gatgtgcaag tcaatgaagc cgatgcgttg tacaccaacc ctgttcaggc
      481 gcgtgaattt gccgaggcaa ccggaattga ttccctggcg gtcgccatcg gcacggctca
      541 cggtatgtat gccagcgcac cggcgcttga tttttctcga ctggagaaca ttcgccagtg
      601 ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta tcaactaagg atattcagca
      661 aaccatcaaa ctggggatat gcaaaatcaa cgttgcaaca gagctaaaaa atgccttctc
      721 gcaggcgtta aaaaattacc tgacagagca ccccgaagcg accgatcccc gggattattt
      781 gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg
      841 cgagggcagg gcataacgca cctgccattt aacaaggaaa aaacatgaaa acgttaattg
      901 cccggcataa agctggtgaa catattggca tatgttcagt ctgttctgca catccgttgg
      961 ttatcgaagc ggcgctggca tttgatcgca acagcacgcg taaagtgctg attgaagcaa
     1021 cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat gacaccggca gactttcgcg
     1081 aatttgtttt tgcgattgcc gataaagtcg gatttgctcg tgagcgtatt attctcggtg
     1141 gcgaccatct gggaccaaac tgctggcagc aagaaaatgt ggatgcggcg atggaaaaat
     1201 ccgtcgaact ggtaaaggca tatgttcgtg ccggcttcag taaaattcat cttgatgcgt
     1261 caatgtcttg cgcgggggat cctataccgt tagccccaga aacggttgcg gaacgagctg
     1321 ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg ccagcgtgag caactgagct
     1381 atgtcattgg caccgaagtt ccggttccgg gcggtgaggc cagcgccatt cagtcagtac
     1441 atatcaccca ggttgaagat gccgccaata ctttacgcac gcatcaaaaa gcgtttattg
     1501 cccgcggtct ggcagaggcg ttaacacgcg tgattgccat cgtggtgcag ccgggtgtgg
     1561 aatttgacca tagcaatatt attcattatc aggcgcaaga agctcaggcg ctggcgcaat
     1621 ggatagaaaa aaccaaaatg gtttatgaag cgcattccac cgattaccag actcagacgg
     1681 cctatcggga attagttcgc gatcattttg caattttgaa agtcggtccg gcattaacct
     1741 ttgctttacg tgaggcgata tttgcgctgg cgcaaattga gcaggaactt atcgccccag
     1801 aaaatcgcag cggttgcctg gcggtaattg aagaagtgat gtttgacgaa ccgcaatact
     1861 ggaaaaaata ttatcgtacg ggttttaacg attcattact ggatattcgt tacagcctgt
     1921 cggatcgtat tcgttattac tggccgcata gccggattaa aaatagcgtc gaaacgatga
     1981 tggtgaatct gcaaggcatg gaaatccctc tgggcatgat tagtcagtat cttcctaaac
     2041 aatttgaacg cattcagtcc ggggaattat cagcaatacc gcatcagttg attatggata
     2101 aaatttatga tgttttgcgc gcctatcgct acggctgtgc ggaataagga cggtatatga
     2161 ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg tagcgaagtt ttaacccata
     2221 tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac ctggccacag gcattaattg
     2281 ccagagaagc agaattccct accgggataa tgcttgagca gcacgccatt gcaataccgc
     2341 attgtgaggc gattcatgct aagtcgtcag ccatttatct gttaaggcca acaaataaag
     2401 ttcattttca gcaagcggat gatgataacg acgtggcggt atcgttggtg attgcgttaa
     2461 ttgtggaaaa tccgcagcag caattgaaac ttttacgctg tttatttggc aagttacaac
     2521 agcccgagat cgtcgagaca ctaatcactc ttcctgaaac ccagttaaag gaatacttca
     2581 caaagtatgt tttagattca gacgaataaa tccctctgta acaataaaaa ggactattta
     2641 tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc gacctctacg atggcggcgg
     2701 aagaaattaa agagttgtgt cagagtcata atattcctgt tgaattaatc cagtgtcggg
     2761 ttaatgaaat agaaacctat atggatggcg tgcatttgat atgcaccact gccagggtgg
     2821 atcgaagttt tggcgatatt ccgttagttc acggcatgcc ttttgtttct ggtgtcggta
     2881 tcgaagcatt acaaaataaa attctgacta tcttacaggg gtgacctatg ttttcagaag
     2941 tcatgcgtta tattctcgac ctcggcccta cggtgatgct gccgattgtc atcattattt
     3001 tttctaaaat attaggcatg aaggcaggcg attgctttaa agcaggtctg catatcggga
     3061 ttggctttgt tggtattggc cttgtgattg gcttaatgct ggattccatt ggcccggcgg
     3121 cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt ggtcgatgtc ggctggccgg
     3181 gctcttcacc catgacctgg gcgtcgcaaa ttgcgctggt ggcgattccg attgcgattc
     3241 tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt ggtaaatgtt gatatctgga
     3301 atatctggca tatgaccttc accggcgcgt tgctgcatct ggcaaccggt tcatggatga
     3361 tagggatggc gggtgtggta attcacgcgg cgtttgttta taagctcggc gactggtttg
     3421 cccgcgatac ccgaaatttc tttgagctgg aaggcattgc tattccgcac ggtacgtcgg
     3481 cgtatatggg gccgattgcg gtgctggtcg atgctatcat cgagaaaatc ccaggcgtta
     3541 accgaattaa atttagcgcc gatgatattc agcgcaaatt tggccctttt ggcgaaccgg
     3601 tcaccgtggg ttttgtgatg gggctgatta tcggcatcct cgcgggttac gatgtcaaag
     3661 gtgtattgca gctggcggta aaaacggcgg cagttatgct gctgatgcca cgggtgatta
     3721 aacccatcat ggatggttta acgcccatcg ctaagcaggc ccgtagtcgt ttacaggcga
     3781 agttcggcgg tcaggagttc ctgattggcc ttgatccggc gttgctgctg ggacatacgg
     3841 cggtggtatc ggcaagcctg atttttattc cactcaccat tttaattgct gtttgtgtgc
     3901 cgggtaatca ggtgctgccg tttggcgacc ttgccaccat cggcttcttt gtggcgatgg
     3961 cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat ctcgggtgtc atcattatga
     4021 gcatcaccct gtggatcgcg acgcaaacta ttggtttgca cacccaactg gcggctaatg
     4081 ctggggcgtt aaaagccggg ggcatggtgg cttcaatgga tcagggcggt tctcccatta
     4141 cctggttact gattcaggtt ttctccccgc aaaatattcc cggtttcatt attatcggtg
     4201 ccatttatct taccggtatt ttcatgacct ggcgtagagc gcgtggcttt attaaacaag
     4261 agaaagccgt tctcgcagaa taatttttac ctgaggggga ggttatcccc cttaataatc
     4321 aggagttttt atgaaatcag tggtgaatga tactgatggt atcgtgcgcg ttgcagaaag
     4381 cgtcattcct gaaattaaac atcaggatga ggtgcgggta aaaattgcca gctcgggctt
     4441 atgtggttcc gatttaccca ggatatttaa aaatggtgca cattattatc caataacgtt
     4501 aggtcatgaa tttagcggct atattgatgc ggtgggatcc ggtgttgatg atttacaccc
     4561 tggcgatgtg gttgcctgtg tgccgttatt accctgtttt acttgtccag agtgtttgaa
     4621 agggttttat tcccagtgcg caaaatatga ttttattggc tcgcggcgtg atggtggatt
     4681 tgccgaatat attgtcgtta agcgaaacaa tgtctttgca ctacctgcgg atatgcctat
     4741 tgaggatggc gcttttattg aaccgattac cgttggcctg catgcgttcc atttagcgca
     4801 aggatgtgag aataaaaacg ttattattat tggtgccgga accattggcc tgctggcaat
     4861 tcagtgcgct gtcgcgctgg gagcaaagag tgtgacggct atcgacatta gctcagaaaa
     4921 actggcactg gcaaaatctt tcggagcgat gcaaacattt aacagccgtg aaatgagcgc
     4981 gccgcaaata cagggcgttt tacgcgagct gcgctttaat cagcttatcc tcgagacggc
     5041 tggcgtcccg caaaccgtcg aactggcggt agagattgcc ggtcctcatg cccaactggc
     5101 gctggtgggc acgttgcatc aggatctgca tttaacatct gcaacgtttg gcaaaatatt
     5161 gcgtaaagag ctgacggtta tcggcagttg gatgaactac tccagccctt ggccggggca
     5221 ggagtgggaa acggcgagcc ggttgctgac agaacgtaag ttaagcctgg agccattaat
     5281 cgctcaccgt ggaagctttg aaagcttcgc ccaggcggtg cgtgacatcg ctcgtaatgc
     5341 catgccgggc aaagtgttgc tcattccctg aaaccgcggg ccagcgtgat gcaggcccgg
     5401 tattgtgcaa aacagatcat tcaccaatgg tcccccttcg tttacactag ccacaattga
     5461 atatgggtaa atgacgatga attcattcga gcgaaggaat aagatcatcc aattagtgaa
     5521 tgaacaggga accgtgcttg ttcaggatct ggcgggagta tttgctgcct cggaagcgac
     5581 aatccgtgcc gatttgcgct ttctcgaaca aaaaggcgtg gttacgcgct ttcatggcgg
     5641 tgcggcgaaa ataatgtccg gtaatagcga aaccgagacc caggaagtcg ggtttaaaga
     5701 gcgatttcag ctcgccagcg cgccgaaaaa cagaatagcg caggcggcag tcaaaatgat
     5761 ccacgaaggg atgaccgtta ttctcgacag cggaagtacg acaatgctta tcgccgaagg
     5821 attaatgacc gccaaaaata tcacggtgat taccaacagt ctcccggctg cgtttgccct
     5881 ttccgaaaat aaagacatca ctttggtggt ttgtggtggt acggtgcgcc ataaaacgcg
     5941 ctcgatgcat ggttccattg ctgagcgttc attgcaggat attaatgccg atttaatgtt
     6001 tgtcggtgct gatggtattg atgcggttaa tggtattacg acctttaatg aaggttattc
     6061 cgttagcggt gcgatggtga cagcggcgaa taaagtgatt gccgttctcg attcatcgaa
     6121 atttaatcgt cgcggtttta atcaggtact gccaatcgaa aaaattgaca ttattattac
     6181 cgatgatgct gtgtctgagg tggataaact ggcattgcag aagacacggg taaaattaat
     6241 tacggtgtag tattgttctg tatgccggat aaggtgttta caccgcatcc ggcattacgg
     6301 attctctctg gttcaacaga gaatagtttg attaacgcaa caatggacaa tctggtggta
     6361 atcgacaacg caacgccgcc ggaagtattt caatatggaa gttttgccca ctcaacggtt
     6421 cgccatcaag attaaaagtg atttcgtgtg gggcttgtat atcaaaccac gacgaagctc
     6481 cttcgataat attcgggtta tcttcgtcag attttaaggt tgatacgaga gtcggaataa
     6541 tttcatcgcc ggtaaaaatg cgcagttgca gcaagccatc gttaattaac gcgttcgggc
     6601 acaattgttg accgccaccg gcctgacgcc cgttaccaat accaatgacc agggcgtcac
     6661 cttgccagtg aaagttttca ccgcggattt cacaacggtc cggttgcaga gtgtccatgc
     6721 gcattaagcc atgaatgatg taagagacgc cacccagcgc ggcttttaat ttttccggcg
     6781 tttctgtggt aatacgcgtc ccaaacccgc ctgtcgccat attaataaaa caggtttgtt
     6841 tattgacctg cgccatatct atcgcaatgg cgttaccggc aattgccagt ttcagcgcct
     6901 tatccagtgc ctcaggaatc cctacactgg tggcaaaatc attggcggtt cctaatggta
     6961 aaattcccag cgcgggtatg tcatccccct cacactgaat caacgccgta gaaacttcat
     7021 taatggtgcc atcgccacca ccggcaatca ccgttgcgac gcccaacttc cgtgcctcct
     7081 ctacatatcg tgcggcatcg cctttctccc aggtgacccg cacatggatc gtcattcctt
     7141 cctcacgcaa cagcataatt gcttcgcgca agggtagatt gtcagtactt ttgccattaa
     7201 gaatcagtaa gctggcggga aattctgcca tgatcgtatg tgcctttatg attggactgt
     7261 agatagtgta gagcagaaaa gaaaaggtgg agtcagaaca ggatgaaagt cggaggataa
     7321 aaagtcagtt caagtatgga ggattcagtg gttaccacca ggagcttgca atgggaacag
     7381 cgaatattaa cggtagccac cggtaaattt caaaaaagaa catataccta atattcaact
     7441 aaacagtggc atcttcaata tactattaaa aatatattaa cccccccatg gggttacctg
     7501 gaagggcatc cgtgtatgta attccta
//
LOCUS       DAZGHF010000183         7512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGHF010000183 DAZGHF010000000
VERSION     DAZGHF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.184"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            100..305
                     /locus_tag="ACHK3A_004484"
                     /pseudo
     CDS             100..305
                     /locus_tag="ACHK3A_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(394..1530)
                     /gene="yibH"
                     /locus_tag="ACHK3A_004485"
     CDS             complement(394..1530)
                     /gene="yibH"
                     /locus_tag="ACHK3A_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8742479.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1533..1895)
                     /gene="yibI"
                     /locus_tag="ACHK3A_004486"
     CDS             complement(1533..1895)
                     /gene="yibI"
                     /locus_tag="ACHK3A_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO8742480.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2432..4345
                     /gene="mtlA"
                     /locus_tag="ACHK3A_004487"
     CDS             2432..4345
                     /gene="mtlA"
                     /locus_tag="ACHK3A_004487"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO8742481.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4710..5858
                     /gene="mtlD"
                     /locus_tag="ACHK3A_004488"
     CDS             4710..5858
                     /gene="mtlD"
                     /locus_tag="ACHK3A_004488"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO8742482.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIRK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5858..6445
                     /gene="mtlR"
                     /locus_tag="ACHK3A_004489"
     CDS             5858..6445
                     /gene="mtlR"
                     /locus_tag="ACHK3A_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO8742483.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6456..6665)
                     /gene="yibT"
                     /locus_tag="ACHK3A_004490"
     CDS             complement(6456..6665)
                     /gene="yibT"
                     /locus_tag="ACHK3A_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO8742484.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            6949..7311
                     /gene="yibL"
                     /locus_tag="ACHK3A_004491"
     CDS             6949..7311
                     /gene="yibL"
                     /locus_tag="ACHK3A_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO8742485.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1937 a         1869 c         1866 g         1840 t
ORIGIN      
        1 tataatgaat acgataatta aaagtgaaga gaaaaatagt tgtatacata tattccatga
       61 acctacattg tgatctataa cttaaatatt tttaaatgtg actgtcatcc ttcagataat
      121 aaagccatca tcgcaataga tggaaaagca ctcctgctct tttttgatta gagccgtggc
      181 agaggaacaa ctcatatcat cattgcgatc tcgacaatgt atagtctgat ccttcggcag
      241 atcaagacgg ataaaaaatc caatgagttc acactcatcc cgaacttctt aacataatgg
      301 atattaacga aaaatcatca cagccgatga aataatatgc aaaaataatt agtccttaca
      361 aggagcaaag ttccataatc tcccccctcc ccctcaatga tccaaataaa gataatgcat
      421 ccaactggtc attctcagca gcactttccg catcaccgaa acatggctga aatggtcgga
      481 gtaaactgcc acttgggcgt agatgccgtc gggtaaggca tcgatatcat cgttagggtc
      541 cagttcaatg gttcccagca caccgtccgt gccaggcacg accgttaatg attgcaatac
      601 cccctgcgcc tgataagaac cgcctggcac gacaggtaaa atactagtca gtttgccgtg
      661 aaacacctgc ccaggtagcg cgttaaacac cacttccgca tcatcgccgg gtttcagacg
      721 taacagcgag ttttgccgaa attgggcgac aatttgccgt ttttgctcgg ggatgaaaac
      781 catcaccgga cgcaacggca aggcagctgc gtatgtacct gggcggatca gtacctgagt
      841 aacgtagcca ttgcttggtg cgcgaatgac agtctgctca aggttatatt ttgcttcagt
      901 aagttgcgct cttaagctca caatctgcga ttgctcgccg ttaaccatac tgtcgagctg
      961 gctctggatc tgcgcctgct ccgccaccga gcctttcacc agcgcgtcct gcgcgaggaa
     1021 attttgccgc gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg cctggctgcc
     1081 tttcaggtaa cgttgataat ttttaaacag acggtcgcgc tccgctgaaa cctgggtggt
     1141 gttggcctgc gcttcagtga gctgcgcgcg cagcgtcttt atattatgcg tcgccgtcat
     1201 caggtcagcc tgaagtctgt caactcgcgc ctggtaacga accgggtcga gcttaaaaag
     1261 cacctcgccc ttttgaataa gctgattatt tttgtcggtg acttcagtaa caattcccgt
     1321 cacctgtggc gtgatgggga tcgctatcac tgccttttgc gcggtaaaag tgtaagggtg
     1381 gttgtagttc atcaacaaaa tcaaaccact caccagaaac acgcctccca gcgccgcggt
     1441 cgccagcgtc cactgattca ccggaatgcg gaagatttta aagaccgccc acgccagcgc
     1501 cacgtaagtt aaaacaatca atagatccat atttagatct ccgggaacgt ggaatagtca
     1561 gtctttttct cggcggcgag ttgatgctcc agccgggaaa ttcggtcaga aagtgcggcg
     1621 atttccgggt cagtcgcttt ctcctgcgac gcaacatgtg actgcatacc ccagccacgc
     1681 tccggttgat agagcgtcgc ccagatccac aggaacggcc agataacatg cagagtaaac
     1741 aggctcaccc agcccgccgt atgaatagcg tcggcatggg gatgattgcg ctttttggca
     1801 atcagatatg ggatgtcatg tatcgcgatg attccataaa aaatcaccag aaagacgaag
     1861 atcagcactc ccaacgcgaa atagtttaga aacatatctg cctcggattc acgtttatca
     1921 gtgttgtttt ttggctggca gccagaaggg agtcaggctg atattttgac aataatccgg
     1981 gttcgcgatt ctcgccataa caccaaagaa taatttttag aggtgatgag ttgcttagtt
     2041 acataacgat tttatgacga aggcataaca tgctgtagat cacatcaggt gaacgccgta
     2101 agaaaatatc ttgtgattca gatcacaaag atccaacaaa ccaccaaaac aaaaatgtga
     2161 cattagtcac atttaaatgc catttttagc gaaaatcgcc gtcttgttgc ttttttacac
     2221 aagcattttg tgatgagcat cacgtcaatt acctctctac cccctatatt tatgtgaccc
     2281 atatcacaca aaaggccgtc gactggacag ttaaccgatt cagtgccaga tttcgcagta
     2341 tctacaaggt ccggctacct ctgccgccac attaacaaaa aacctcgggc ttccagcctg
     2401 cgcgacagca aacataagaa ggggtgtttt tatgtcatcc gatattaaga tcaaagtgca
     2461 aagctttggt cgtttcctca gcaacatggt gatgccaaat atcggcgcgt ttatcgcgtg
     2521 gggtatcatc accgcgttat ttattccaac agggtggtta ccgaacgaga cgctggcgaa
     2581 gctggtcggg ccgatgatca cttatctcct gccgctgctg atcggttata ccggtggtaa
     2641 gctggtaggc ggcgaacgtg gcggcgtagt cggtgccatc accaccatgg gcgttatcgt
     2701 cggcgcagac atgccgatgt tcctcggttc tatgattgca ggtccgctgg gcggctggtg
     2761 cattaagcac ttcgaccgct gggtagacgg taagatcaaa tccggttttg agatgctggt
     2821 gaataacttc tccgcaggca tcatcgggat gatcctcgct attctggcat tcctcggcat
     2881 tggcccgatt gttgaagccc tgtccaaaat gctggctgcg ggcgttaact tcatggttgt
     2941 ccatgacatg ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc tgttcctcaa
     3001 caacgccatt aaccacggta tcttctcgcc gctgggcatt cagcagtccc atgaattggg
     3061 taaatccatc ttcttcctga ttgaagctaa cccaggtcca ggtatgggcg tgctgctggc
     3121 gtacatgttc tttggtcgtg gcagtgctaa acaatctgcg ggcggtgcgg caatcatcca
     3181 cttcctgggt ggtatccacg aaatttactt cccgtatgtg ttgatgaatc cgcgtctgat
     3241 cctggccgtt atcctcggcg gtatgactgg cgtgttcacg ctgactatcc tgggcggtgg
     3301 tctggtttct ccggcatctc cgggttctat ccttgctgta ctggcgatga caccaaaagg
     3361 tgcttatttc gctaacatcg caggtgtgtg tgcggcgatg gctgtctcct tcgttgtctc
     3421 tgccattctg ctgaaaacca gcaaagtgaa agaagaagat gatattgaag cagcaactcg
     3481 tcgtatgcag gacatgaaag ctgagtctaa aggtgcgtct ccgctgtctg cgggcgatgt
     3541 gactaacgac ctgagccacg tacgtaaaat catcgttgcc tgtgacgccg gtatgggttc
     3601 cagtgcgatg ggcgcaggtg tgctgcgtaa gaaaattcag gatgcaggtc tgtcgcagat
     3661 ttcggtcact aacagcgcga tcaacaacct gccgccagat gtggacctgg tcatcactca
     3721 ccgtgacctg accgaacgcg ctatgcgcca ggttccgcag gcacagcata tttcgctgac
     3781 caacttcctc gacagcggct tgtacaccag cctgaccgaa cgtctggttg ctgcccagcg
     3841 ccatactgaa aacgaagtaa aagtgaaaga cagtctgaaa gacagctttg acgattccag
     3901 tgctaacctg ttcaagctag gcgcggagaa catcttcctc ggtcgcaaag cggccaccaa
     3961 agaagaagcg attcgttttg ctggcgagca gctggtgaaa ggcggttacg ttgagccaga
     4021 atacgttcag gcgatgctgg atcgtgaaaa actgaccccg acttatctgg gtgagtctat
     4081 cgcggtgcca cacggtacgg ttgaagcaaa agatcgcgta ctgaaaacgg gcgtggtgtt
     4141 ctgccagtac ccggaaggcg tgcgcttcgg tgaagaagaa gatgacattg cccgtctggt
     4201 gattggtatt gctgcccgta acaacgagca cattcaggtt atcaccagcc tgaccaatgc
     4261 actggatgat gaatccgtca tcgagcgtct ggcacacacc accagcgtgg atgaagtgct
     4321 ggaactgctg gcaggtcgta agtaatacaa tctcaccctc tccacatgga gaaggtgggg
     4381 cttattgcct gatgcgctac gcttatcagg cctacaaaac aaagcacagt ttgttgaatt
     4441 tgcacgttct tgtaggccgg ataaggcgtt tacgccgcat ccggcgctgc ccctctccac
     4501 atggagaaga tgggctaatt gcctgatgcg ctacgcttat caggcctaca aaaacaaagc
     4561 acaatttgtt gaatttgcac gttcttgtag gccggataag gcgtttacgc cgcatccggc
     4621 gcggcccctc tccacatgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc
     4681 gggtaaaaac attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata
     4741 tcggtcgtgg ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg
     4801 atgtcaatca ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg
     4861 tcggtgaaac cgagcaggta gataccgttt ccggcgtcaa tgccgtcagc agcattggtg
     4921 atgatgtcgt tgatctgatt gctcaggttg atttagtcac taccgccgtt ggcccggttg
     4981 tgctggaacg tattgctccg gcaatcgcca aagggctggt gaaacgtaaa gaacaaggta
     5041 atgaatcccc gctgaacatc atcgcttgtg aaaacatggt ccgcggcacc acacagctga
     5101 aaggccatgt gatgaacgcc ctgccagaag atgccaaagc gtgggtagaa gaacacgttg
     5161 gctttgtcga ttccgccgtt gaccgcatcg tgccgccttc ggcttctgca accaacgatc
     5221 cgctggaagt gacggtagaa acattcagcg aatggattgt cgataaaacg cagttcaaag
     5281 gcgcgctgcc aaacatccct ggcatggaat taaccgacaa tctgatggca tttgtcgaac
     5341 gtaaactctt caccctgaat acaggtcatg ctataaccgc gtacctcgga aaactggccg
     5401 gtcatcagac cattcgtgat gcgattctcg acgagaaaat ccgcgcggtg gtaaaaggtg
     5461 cgatggaaga aagtggtgca gtattgatca agcgctacgg ctttgacgca gacaaacatg
     5521 cggcgtacat ccggaaaatc ctcggtcgtt tcgagaaccc gtacctgaaa gatgatgtag
     5581 agcgcgtagg ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtcta atcaagccac
     5641 tgctcggaac gctggaatat ggtctgccac ataaaaacct gattgaaggt attgccgctg
     5701 caatgcactt ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg
     5761 acaaaggtcc gcaggcggcg ctggcacaga tttccggtct tgatgccaac agcgaggttg
     5821 tatccgaggc ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac
     5881 cctgcgcccg aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt
     5941 tgaaaaccgt gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac
     6001 cgccgtcgag cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga
     6061 cgattacgcg gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga
     6121 tctttctgtg cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga
     6181 agatgcggaa ctgctgatgg cattgcgtga agagctaaat cacgacggca acgagtacgc
     6241 gtttaccgac gacgaaatcc ttggaccctt tggtgaactg cattgcgtgg cggcgttacc
     6301 accgccgcca cagtttgaac ctgcagactc cagtttgtat gcaatgcaaa ttcagcgcta
     6361 tcaacaggct gtgcgatcaa caatggtcct ttcactgaca gagctgattt ccaaaatcag
     6421 cttaaaaaaa gcctttcaaa agtaagtact ttcgcttact tctcctctac ctgtttcgga
     6481 cgataaagct gacgataata ctccagacgt tgtagatata acggcacgct ttcatcaggt
     6541 attccggacg gaatcgcgtt acggggaggt agttttttca gatactcccg gaacgcctgg
     6601 ctcgatgcca tgaaatctac ggctttatcg ataagaagcg gaacgttttc acctaatttg
     6661 cccatgatga tatctccgta ttaccccgcg ccgggaatgc gcggccgcca attttagttt
     6721 agatcccggt ataaatgaca ttaaggaaaa cgtgctcaat cctcaataat cacctgcttc
     6781 tgttgactat cttttttctt taaatttctt aattaatcaa taaattgcgt agtttttaag
     6841 catgttaatg tttacgttat agaactattg caatgtcatc agttgtgaac aaagcacctg
     6901 gtcgcgcata ctaggggcta taaatttatc tttatcagaa gccatcacat gaaagaagtc
     6961 gaaaaaaacg aaatcaaacg tctcagcgat cgtctggacg ccatccgcca ccagcaggcc
     7021 gatctgtcgc tggttgaagc cgcagacaaa tatgccgaac tggaaaaaga gaaagccact
     7081 ctggaagcag aaattgctcg cctgcgcgaa gttcacagcc agaaactcag taaggaagca
     7141 caaaagctga tgaagatgcc attccagcgc gcgattacca aaaaagagca ggctgatatg
     7201 ggcaagctga agaaaagtgt tcgcggactg gtcgttgtgc acccaatgac cgcgctgggc
     7261 cgcgaaatgg gcctgcaaga gatgacaggg ttctcaaaga ctgcgtttta agttcctttc
     7321 tcgtcgctgt aatcacagcc tctcaccatt tactcgctcc tgctggcaca tgcaggagcc
     7381 tgtttcagag atcgaatatt tataacgttc ttaacattat atattcaatc tcctctattc
     7441 tttttagcgc acatgccagg ctaaattctg aaaatcaggt acaagttaaa gaacgatgta
     7501 tcaatattaa ca
//
LOCUS       DAZGHF010000184         7482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGHF010000184 DAZGHF010000000
VERSION     DAZGHF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.185"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(172..774)
                     /gene="fimB"
                     /locus_tag="ACHK3A_004492"
     CDS             complement(172..774)
                     /gene="fimB"
                     /locus_tag="ACHK3A_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO8742486.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2226..2942
                     /gene="nanC"
                     /locus_tag="ACHK3A_004493"
     CDS             2226..2942
                     /gene="nanC"
                     /locus_tag="ACHK3A_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO8742487.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2962..4068
                     /gene="nanM"
                     /locus_tag="ACHK3A_004494"
     CDS             2962..4068
                     /gene="nanM"
                     /locus_tag="ACHK3A_004494"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO8742488.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLAPVASPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRVYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4133..5113
                     /gene="nanS"
                     /locus_tag="ACHK3A_004495"
     CDS             4133..5113
                     /gene="nanS"
                     /locus_tag="ACHK3A_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO8742489.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTCDYSSHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILRFWRER"
     gene            complement(5121..5771)
                     /locus_tag="ACHK3A_004496"
     CDS             complement(5121..5771)
                     /locus_tag="ACHK3A_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO8742490.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHQDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFSPEELKALMDANKSN"
     gene            5908..6051
                     /locus_tag="ACHK3A_004497"
                     /pseudo
     CDS             5908..6051
                     /locus_tag="ACHK3A_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(6759..6842)
                     /locus_tag="ACHK3A_004498"
                     /pseudo
     CDS             complement(6759..6842)
                     /locus_tag="ACHK3A_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025205684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7025..>7482)
                     /locus_tag="ACHK3A_004499"
     CDS             complement(7025..>7482)
                     /locus_tag="ACHK3A_004499"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFO8742491.1"
                     /translation="MPDMRLFKSKNTKLELRIRKPLFARGFRYRLHNSGLPGRPDMYF
                     PKYNAVIFIHGCFWHGHDNGCPISHTPKNNESYWQNKINKNQERDVKNQYVLASKNIR
                     VLIIWECAIKLKSIDFVDFIDYIDEWLRGGANSTQMSAKEPMPTFEFPK"
BASE COUNT         2236 a         1557 c         1592 g         2097 t
ORIGIN      
        1 ttttgaagaa cataagccat tgtattttgt atggatatgg ttaacttaat gtttattatg
       61 ataattaaga atcacactta caataacatc aattaataaa tggttaatca tttttgatat
      121 atcgtaagaa taatgtagtt tttaacacca tccctggtat ctcaactatc tctataaaac
      181 agcgtgacgc tgtctccctc tggctctatc ccagatgccg taaaaacgcc ctgcattgct
      241 ggcggtatac cagacagtat gacgaatatt gcgatgccca agataatcct ggataagtcg
      301 cgtatctatt cccatattcg ccaaagcaaa accacacgaa tggcgtaaca tgtgcggatg
      361 aatctccagt gacaacccgg cattaccacc ggaagtcgag ataatatggt aaaactgttg
      421 ccgagaaagt ggattcccct tacgtgataa gaatacccac tcgctctcag catgcggata
      481 agaagtacgg atactcaacc agttttttaa agcctgaatt tctttgttca atagcgggtg
      541 cgttgttgaa aagccttttt ttaatcgatg gatatatata cactttgcct taagatcaat
      601 atccgaaatc ctcaatcggc aaatttcact cgcccggaaa ccatgaataa aacaaagcaa
      661 agtcagacaa taattacgtg ctgcatgagg cccggtattt gctgctttaa ggagtgattc
      721 gatttcacta tgggtcagga agttcctttt tttgttatca gccttattct tcatcgtttt
      781 cccttataat tacagacgcg cactagctgt gctgggttag caataatcca acatttttat
      841 cctgattatg tttataaaag cgaacgtttg cttaataact aacgtaagtg gaccagttct
      901 tctgagcgaa cttaaatgga gtagcgatag ttacttataa taactttgcc atacaaacgc
      961 catgctcttg catgctatgt acctttatat atttatcaat ctgatacgaa tcgaataata
     1021 accctcatca ttaatgcaac taatcttatc tatatatcat gtgatatgtt ctgtaacaag
     1081 taaaatcaac ataaaaaatt cagcaaaatt taatattcgt tcaactagcc gtctcacata
     1141 tgacattatt agtcaactgc atttgccaca aaatggctat ttcatggaac gcagtaattt
     1201 cttagtggct attatcatgc taattcttag atccctagcg attattcctg cctggttcgt
     1261 cagcttaggc catgagattg ttacattccc ttttatcctg gagatatggg ggaacgcagg
     1321 cgacgctttt tcatctacat ccgttggtaa ctatggcttt ggtcactacg acttaaacct
     1381 tcctcaatca cagctattcg ccgggcgata cggcaaacaa actaagtgaa acatcatcct
     1441 gaaaacacca acatcaacaa gcctctccag atcgacatca ggagtgacca gttacaagcc
     1501 acaaaccaag aaacatattt catgatgaga attatgctca gcctgatggc gggtaaacac
     1561 ttattttcac caagctcact gagttagctt gcaaagcttt ctttgtttag attctaaata
     1621 aattttttat aaaacagaca gttaaaataa aatctttaat ttttgaagac caccgcaagt
     1681 gttcgtctgg cttcacatgg catctgcctc ttagaaaaag atcgacatat tttgtgacac
     1741 gaattgcaaa tctcgttttg ttgtatggat tgcgtgattt ttgatctggt ataataggta
     1801 taaaggtgca ccgcgatagt caatgagaca gggcatctcg caatctatgg caaacatcac
     1861 ttcagttctt tctcatcggg tgatgaaaac gcacttcagt ctgaaaggaa tatgaaaatg
     1921 agatcaacag acactctatt ttatgactct gggtaaaatg gattgagtaa gtgatatagc
     1981 ttacgaacat tcaaatcaat taaacatcag aagagatttt atactcaggt atttaatctg
     2041 gttctctgtt tatttaaata atgtggaaag agaattttca caggagacct tatacaaaaa
     2101 atataaaata agctacccgt tgccaaagac actataagcc tggcaaaaaa tattacacaa
     2161 cataaatgct aattgtttat gcgggctttg tattgctttc tgtatcctac aaatgagtga
     2221 aatttatgaa aaaggctaaa atactttctg gcgtattatt actgtgcttt tcgtcaccat
     2281 taatttctca ggctgcgaca ctggacgtac gtggtggata tcgtagcgga agccatgcct
     2341 atgagactcg actcaaagtc agtgagggat ggcaaaatgg atggtgggca agcatggaaa
     2401 gtaatacctg gaataccatt catgataata aaaaggaaaa tgccgcactc aatgatgttc
     2461 aggttgaagt taattacgcg attaaacttg atgatcaatg gacggtgcgc ccgggaatgt
     2521 taacgcattt tagcagcaac ggcacacgct acggacccta cgtaaaactg tcctgggacg
     2581 cgacaaaaga tcttaatttt ggcattcgct atcgttacga ctggaaagct taccgacaac
     2641 aagacttatc cggtgatatg tctcgtgata acgttcatcg ttgggatgga tatgtcactt
     2701 accatattaa tagtgatttc accttcgcat ggcaaacgac gctatacagc aaacagaatg
     2761 attatcgcta tgcaaaccac aagaaatggg cgacggaaaa tgcctttgtt ctacaatacc
     2821 atatgacgcc cgatattacg ccatacatag aatatgacta ccttgaccgt cagggtgttt
     2881 acaacggcag agataattta tcggaaaaca gttatcgcat tggtgtgtca tttaaactgt
     2941 agtagacagg agacagtcac aatgaataaa acaataacgg cgcttactat cataatggct
     3001 tcatttgccg caaacgcgtc tgtattaccg gaaactcctg tgccatttaa aagtggtacc
     3061 ggagcaattg ataacgacac tgtctacatt ggtttaggta gcgcaggtac ggcatggtac
     3121 aagctggata cacaggccaa agataaaaaa tggacagcgt tagctgcatt ccctggcgga
     3181 ccgagagatc aagcaacctc tgcatttatt gatggcaatc tgtatgtgtt tggcggcatt
     3241 ggcaaaaaca gcgagggctt gactcaagta tttaatgacg tacacaaata caaccccaaa
     3301 accaatagct gggttaaatt gatgtcgcac gcgccgatgg gcatggcggg ccatgtgact
     3361 tttgtacaca acggcaaggc ttatgttact ggcggtgtta accagaatat cttcaatggc
     3421 tattttgaag atctcaacga agctggaaaa gattcaaccg ctatagataa aatcaacgcc
     3481 cactattttg acaaaaaagc agaagattat ttcttcaata agtttctgtt gtcttttgat
     3541 ccctcaacac agcaatggag ttacgctggc gaatctccct ggtacggaac ggctggtgcg
     3601 gcggttgtga ataaaggtga taaaacctgg cttattaatg gcgaagccaa accaggattg
     3661 cgaacggatg ccgtatttga acttgacttc accggtgata atttaaaatg gaataagctt
     3721 gctcccgtcg catcaccaga tggcgtcgct ggcggttttg cggggataag caatgattct
     3781 cttatatttg ccggaggggc cggattcaaa ggttcgcggg aaaattacca aaacggtaag
     3841 aactatgcgc atgaaggcct gaaaaaatca tatagcactg atattcatct ttggcataac
     3901 gggaaatggg ataaatctgg tgaattatcg caaggtcggg tctacggagt atcattgccc
     3961 tggaataata gtttattaat tattggcggt gaaactgcag gcggcaaagc ggtgacggat
     4021 tcagttttaa tttctgtgaa ggataataaa gtcacagtac aaaactaacg cttcagggcc
     4081 tcggtaaagg ggccctgcta tcaatttgtc atttgtatta cgcgaaaatt atatgaacgc
     4141 aataatatcg cccgattatt actatgttct taccgttgct ggtcagtcta atgccatggc
     4201 gtatggcgaa ggactgccat taccggacag ggaagatgcg cctcatccca gaattaaaca
     4261 attagcgaga tttgcgcata cgcatcccgg aggcccgtca tgtcacttta acgacattat
     4321 tccactgact cactgcccac acgatgttca ggatatgcag ggttatcacc atcctctggc
     4381 aacaaatcat caaacacagt acggcaccgt tggccaggca ctgcatattg cacggaaatt
     4441 actgcccttt attcctgata acgcaggggt tctcatcgtt ccgtgttgcc gtggcggatc
     4501 ggcttttacc gcgggcagcg aagggacata ttcagaacgg cacggagcca gccatgatgc
     4561 ttgtcgttgg ggaacggata ctccgctgta ccaggattta gtcagcagaa cgcgagccgc
     4621 actggcaaaa aatccgcaga acaaattcct cggcgtatgc tggatgcaag gcgaatttga
     4681 cttaatgacc tgtgactact cgtcacaccc tcaacacttt aatcatatgg ttgaagcctt
     4741 tcgtagggat ctaaaacaat accattctca gcttaataat attactgacg caccgtggtt
     4801 ttgcggcgat accacctggt actggaaaga aaatttccct catgcgtatg aagcgattta
     4861 tggcaattat caaaataatg ttttagccaa tattattttc gtcgacttcc agcaacaagg
     4921 tgaaagagga ctgacgaacg cgcctgatga agatccggac gatttaagca cgggatatta
     4981 cggttcagcg taccggtcac cggagaactg gacgacggca ctgcgaagca gtcatttcag
     5041 cgcggcagcc cgtcggggga ttatttctga caggtttgta gaagcaattt tgcggttttg
     5101 gcgcgaaagg tgagcgttca ttaatttgat ttatttgcat ccatcaacgc ttttaactcc
     5161 tccggtgaaa acggtggttt gcgtcggtgc agcatcgcat ggcaattagg gcacacaggc
     5221 agcaaatcat taactggatc cagctggtaa tcctctttaa tggctgaaag cggcaccagg
     5281 tgatgcatat ggataaagcc ttcagcgata tcaccataga tcttcaccag atcaacgccg
     5341 catacgttgc actggcatcc atgatgttcg accgctttat ctcgggcctt tttatcgcgt
     5401 tcatagcggt ttactgtcac ctttatcgcc gcaccttcaa ggtatgtctc ctgcgacggc
     5461 aactcatccg ggaacagaac gtcggctatg ggagccgcag cggctttatc ctgatgaata
     5521 tgaaatccct gctctatgag tagtttgacg catttagatt ttattccgcc ggtgaaatcc
     5581 gcaggggtaa attgcgtccc cgtcatcaat gtcgctgcga tgccagcaat cgccttcgag
     5641 gggtaacgtc ggccatgata ttcaatctca tacaaccgcg caggctggaa ctgatgagtt
     5701 tctcccgcat cccataaatg gatagcttcg atgatatacc gccgcggaag atgatcaggg
     5761 agtttattca tggagaatgt ccttatacgt tctcagaaca ggcagcaaaa aaacaaatga
     5821 gatcaaggta atatatcttc acttgcaggc ttgaacagca gaaaatttgc gagcatgatc
     5881 gcaattattt cactgccagg gatatcaatg gcttccagta aatcgctaca gcaggcaatt
     5941 gccaatataa aaatctggca caagggtgaa cagcgcgcgc cgcataagcc attgttattg
     6001 ctatacgtat tagcgggata cctgaatgga catccgcgcc ttttcgatta ttgtagtgga
     6061 acagtctacg aggatgcagt tcgaagtgag taagtaaagg ttgctggaaa cgatcaatgc
     6121 cgccatacaa tgatttttga ggatcaacaa tgagtaataa aaactacgaa agtcaccgca
     6181 aagcgattgt tagtaaaggt ataccacccg ctctattaaa taggctcacc aattcggatg
     6241 ttcaggtgat caataccttc ttaacacgag tgagcaaact ggaactatct caacaagaga
     6301 aagactggat cataaaaatc atctctatgg tttagacaat ttcagccttg tagcctttca
     6361 agggttttgt gtgggtacaa acattatcga atcgggatat agccgcagct gtagctgtat
     6421 ccacatcagt aagttgatgt aggttgactt aggttgagcg aaaaatagcg aaaagccttg
     6481 tgtggcgcgg atttcagtca taaaaaaacg tccgttgacg tctattgatg ttccgatggt
     6541 gcgaaggtcg aaggccggac tcgcctttgc gtttaattcg ttgaaaaata atggtttgtg
     6601 agattttatt ttgaggattt gcccccaaaa tgggacataa aattgaatac tgggtggaag
     6661 atcacaaagt tgcgtaatta ttttccaggt ccgaaacgca ttcaagagca aggcctctcc
     6721 cggcactgcg tcagtcggga tgctaaatct cccacatgta gccctagtaa atcgcgccag
     6781 aatgtcaggc tagtttgcaa attactgacg gcgagcgtta agtggttcag agattgaagc
     6841 ataggatgcc tattgatggt taataattta accaaacact aatagctaat aaaaccccac
     6901 atggaaagag attttttgcg ggtcttgttt tgtaataaat ttcttggcaa ttactttatt
     6961 tcatatatat attagcaaat aacgatgtta gatattacca ctcaagggaa cttgtcataa
     7021 taattcattt cggaaactca aaagtcggca ttggttcctt tgcgctcatc tgtgttgaat
     7081 ttgccccacc tctaagccat tcatcgatgt aatcaataaa atcgacaaaa tcaatacttt
     7141 taagttttat tgcacactcc catataatca gaacgcggat gtttttagat gcaagaacat
     7201 attgattttt gacatctctt tcttgatttt tattgatttt attttgccag tacgattcat
     7261 tatttttagg agtgtgagat atagggcagc cgttatcatg cccatgccaa aaacagccat
     7321 ggataaaaat gactgcattg tatttaggga aatacatatc tggtctacca ggcaaaccac
     7381 tattatgcaa tcgataacga aaccctctgg caaacaacgg cttcctgatt cttaactcaa
     7441 gtttggtgtt ttttgattta aataacctca tatcaggcat at
//
LOCUS       DAZGHF010000185         7479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGHF010000185 DAZGHF010000000
VERSION     DAZGHF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.186"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(70..1206)
                     /locus_tag="ACHK3A_004500"
     CDS             complement(70..1206)
                     /locus_tag="ACHK3A_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO8742492.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
     gene            1882..2090
                     /locus_tag="ACHK3A_004501"
                     /pseudo
     CDS             1882..2090
                     /locus_tag="ACHK3A_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            2077..2266
                     /gene="tnpB"
                     /locus_tag="ACHK3A_004502"
                     /pseudo
     CDS             2077..2266
                     /gene="tnpB"
                     /locus_tag="ACHK3A_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(2269..2457)
                     /locus_tag="ACHK3A_004503"
     CDS             complement(2269..2457)
                     /locus_tag="ACHK3A_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO8742493.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            2992..3133
                     /locus_tag="ACHK3A_004504"
                     /pseudo
     CDS             2992..3133
                     /locus_tag="ACHK3A_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            3356..4093
                     /locus_tag="ACHK3A_004505"
     CDS             3356..4093
                     /locus_tag="ACHK3A_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8742494.1"
                     /translation="MIVCYVILVNRPFETEIAGKKIFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAWRVKDISNSL
                     YMSESLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPYFISV
                     FREYFGITPHQMMINQSGFIESRIAIK"
     gene            4993..>7479
                     /locus_tag="ACHK3A_004506"
     CDS             4993..>7479
                     /locus_tag="ACHK3A_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO8742495.1"
                     /translation="MNKIYALKYSSLTGGLIAVSELSKKVTGKTDRRLMTVSLVLSVT
                     LSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEIKDNNGNAVGTLDVPMIDFS
                     SVSRRGSLTLLSQGYGVSAKHGDLGDVNNASFGYDKNNYTVVKNNKHSGLDFSLHRFS
                     KLITEAAPADINIFGQLSDSSQYTAFYRAGAGTQYIKERSGKQTHIPGTFLTGGTVGT
                     PWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSGSPLFAYDSLSEKWSLAGVTLHNYG
                     VNGYRNNWLLLPEDYIRNIIVADFDPIISFNKNSKEHMSWTYDAAKGVGRIQQDDQQF
                     VMHGNLNGNLNAGKNLYFTGENGIIDLKDNVNQGAGYLQFADDYTVTTSNDSSWSGGG
                     IIVNYGTTVKWGINGVFGDDLHKVGDGTLIINGTGKNEGGLKIGAGTVILEQKAKNND
                     STAFSSINISGGNSRVKLSGDNQIIPDNVSWGFRGGYLDINGKDTEFSRLQAVDYGAA
                     IINSSIDKSLLTLNLSPLKKDEIAVSVKVLDMNAIFQGGHGTAGDLYKTTFYGPTQYY
                     LLKKPKFGSVLMGALKNTSEWQFAGTDLNQAVDMAKNNKLTSSAQASYLYHGKLLGNM
                     DIVIPELTGNDILTLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREF
                     SLNNLNLNNADFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKS
                     AAAGTSSYTGHITLEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLN
                     IHKGGALTAQGGLFTSGSIDIGDASLLLTGT"
BASE COUNT         2188 a         1565 c         1548 g         2178 t
ORIGIN      
        1 gaacatttat taataataaa agcctctgat ataaataaaa actatgtaaa tcgtttaaat
       61 ggatacctgt taaaactgat acatcatacc aacagcaagt atattatcgg ttgcaactcc
      121 agcctttttg gtaaattcgc tttcatcaat aaggttaatt ttgtaatcaa taagaagtga
      181 catgtttttg ttcagaaagt agtttgcgcc aacatcaata tactcaacaa gagactgatc
      241 gccatagttt actattccac ctttaacatc catccctttg gactgaacgt aagccagtga
      301 aggacgcagg ccgaaatcaa acatatactg agccacaact tcaatattcc gtgctttatc
      361 tgcgatgcct gcgctaccaa atggagtcat atttcgggtt tcagagtagt cagctgccag
      421 atacaggtca ttatcattat acttgacccc aacacctgca aactccgcat gttttccccc
      481 ggcaatccag cgaggcgtaa taacttcttt tccttctgaa tctttcgatg tcatgaaacg
      541 tttacggcct tctttttcct gcagactggt tctgtcggca ttagtgtaag cggcaacgat
      601 tccaaaacct tcatagtcat agcttacgga gaaaccatat ccatcaccat tagcttcaag
      661 taaattatca cggtcatttt taccctgata ttgagcagaa aaagtaagtc catcaacaag
      721 cccgaagaaa tctgtattgc gccaggtcag aactccggtt gttctgccgg tcatatagtt
      781 atcagagtaa gtccagctgt caccaccaaa gtccggaagt acgtcagtca gagcccccac
      841 atcataggca acaccatagt ttcgcccata atcaagtgaa ccgagtatat tgtggcgaat
      901 acctgcaaat gccagacgat ttttatcgtt gcgtacttca ctttctgtct taaaccccag
      961 gaactggtat tcccactgac caaagccagt caggctctca ttaatttgtg tttcgccttt
     1021 gaaaccaaga cgtgcaaatg ttctgtcgcc atccatagaa ccatcagaga tgtaatgacg
     1081 ggctttaacg cgaccataaa aatccagttt attaccgtct ttattataga tttctgctgc
     1141 ctgtgttgcc tcacacagca ttaatgcaga aacgacagag cacacaacca tacttaattt
     1201 tcgcataaaa aaatcccctg tgcacatttt tagtatggtt ttaaaaatta aactatggcg
     1261 aaaaaattat cactatgcaa agatgtaaca aaatattgct cagtgatcta tattgttgtt
     1321 tttatttgag gttttatatt ttctagtgta tttgactttt ctttctcaag tgttatttct
     1381 tgacctttat cacagaacca tctccctgga tatccccatc atattgcatg aaaagcaagt
     1441 tttaccaatc ttgattttca gtgttaaata agattaataa ctgtaattct gcaaaaaaaa
     1501 gaacaccata tgtagtatga ctacgtattc cactctgttc gttcacggac tggcatcgaa
     1561 gtctgaggtg tactggcaat agcggataca gtcatttgtt ctttttttaa gcattcatcc
     1621 gatgatactt ttcctgaatg ctaccggagt ggtattcccc tgccgtgagg gagggcgctg
     1681 acggttataa aaatcctgat atatacccat tcccgtatga cagcgatggc gtcacatctc
     1741 tgttctacca tcttcacaga aatatttcgt tcattagcat tccggtattc gtataaccac
     1801 cttcggtgga gaaagaataa acaatccctg gaatgcgatg agcgcttgac cagaaaagca
     1861 tcatattgct tcatggcgca tcagcgacca caggatgccg caaaagccga aaaacggacc
     1921 agagcgcctg caatcattcc tcgcctcagt atctgttgcc catggagtgg ccgataacag
     1981 atcattgtcg gatgagcctt atcctcttcc ttcccggtgg tatccggatg tgctgccagc
     2041 cttctcagtt aaccgatgtt ttccgggctt tttgccatac tgaggactga taacgtattc
     2101 ctggtcgtaa aacccgtcaa tatgcgccgg ggcatcgaca aaactgacac agtacgtccg
     2161 gaaagaactt aacgctgcat ggcacgacaa tgctgccttt gtctttacca acaaggctcg
     2221 ttcccgcata aaagttctac gccgggataa acatggcgta tggctgcgtc aggccttcac
     2281 tacttcacac cggaagtctt ccaccctgac acgaatatac cgtccgggct ctttcgcagg
     2341 tgcaaacagt ttttcacatt cggcgaatgt catttctgaa tcctggtaac gcaaccactg
     2401 aaccggcata ttaccgggtt tgattattgt cgcagttatt ttatatcgtt tccccatatc
     2461 gcctctcatc ctgacaacaa aattaccggc tgagccatca ctccggtaaa gcatgcgaaa
     2521 aaaagtgagc gtctcagcaa tggctcatgc gatgattctg tacgggcacc gatggcacct
     2581 cagaatacaa caaagatatc agagtaacat gtctctctcg ttatttttca gagttcttga
     2641 caccctcctt ctgccacatt gtcaacgcca gtggagactt ctgctgtcag ttttttcatc
     2701 catacagagt gacattaatt atcctgaaca aaacgaaata tcctgaatat atttattttc
     2761 tcctggtgcc ggagacatga tgatgtacag gctcaggtgc ggtgtgaatg taaaatggta
     2821 tgttaattat tcatttctga atgcaaactg aatgcaaact tattccgaat tgaattctcc
     2881 tgacaccaca tgataaaaat gaggatataa taaaatactt ctcttctgga tgtgccatta
     2941 cctgccgttg ctgtaatacg gcgggtattt tttactgaca tatcgggaag ccagcagacg
     3001 ttcgtagcta catccccgaa agtggcacac tgcaaactat aatacgagta aaacatcgat
     3061 caccgtagct gatgtgttca acgatcgtgc actagcgttc tgcgattctc agggactgcc
     3121 tgttctgaga atcggcgagt ccgcgatcgg atacgatgcg atttcgcagt gactcaggga
     3181 ttgcatctga aaaccctccg gctatgtcgg taaatattta tttctatgaa ccgtacgatt
     3241 cagatagcat tcgcccggcc ccgtaagaat aatctccgga caaataagat ttttcctgaa
     3301 aaagcgtcta gtcttttgat atcgttgttt ttatgctaat cagggagtat gtgtgatgat
     3361 tgtctgttac gttattcttg ttaaccgtcc gtttgaaaca gagattgccg ggaaaaagat
     3421 attttttcag aaaaatgacg tcctgttgac agataacatc agccagtcct ttttcctgcg
     3481 ccatcccggt aatgttactg agctccggat tgatgaatgt attgtcagaa aatatatgga
     3541 aagtgccgac aggatccagc actctgatag tgctgtttct gttccgttca ttaaaacaac
     3601 cttctctcac atcgctctgt tacataatgt catgcagaat ctcagtctgc aaaagacatt
     3661 gccacaacaa ttttctgagc agatgttttt tgcctgcctt tccatttttt ccacacacag
     3721 aggtttcatg tttctgctat tcagatgcat aaactctgtt gcgcttaaag tcaggtatat
     3781 tatctgttcc agtccatcac gggcatggag agttaaagat atcagtaatt ctctgtacat
     3841 gagcgagagt ttactgaaaa gaaaacttca ggaggaaaaa gtttctttct cccaaatcct
     3901 gctggatata cgcatgcata acgcccgaca acttatttcc agtaaactgt cagtgaatca
     3961 aatcgcaata cggtgcggtt atgccagcac accgtatttt atcagcgttt tcagagaata
     4021 ttttggcata acacctcatc agatgatgat taatcagtcc ggatttattg agagcagaat
     4081 cgcaattaag taaagtatgt cagtcagcgc ataataaaag tattcttatg cttcattatc
     4141 agtttctgtt tatcactccg gagcactgta gtaactacgg ccccggaaaa cagatggtaa
     4201 tgagttgact gactgatgat attgtggcga catttttacg gatgcgagca aagaatgtat
     4261 tggtccgttt gattatgcag tgattaacaa aactccggga agatgaatga gtatacctct
     4321 cctgcaatcc gtttcctgag aaaacaggtg gtaacacaat attcttaata tgttactgtt
     4381 tcaccacaca accaggttta cggttgtgtg gtgaactata gtaatcccgg gtaacaatcc
     4441 ggagctgttc cttttatact cttcaggctg tgtactcatc cacgccatcc gtttttcagt
     4501 ctcagtgtgt gaataataaa ttacactttg aactgaacgc cagtcaggac catgtcgtat
     4561 ttccgggtgg atgagcatat atcagaaact acaataacta cagataacgg ttttcagtaa
     4621 ttctgtcata acttgccgga atgcaatgtt tttgtgacat ggttcttatt ttttatacac
     4681 aggcaggttt tgtctggatg gaatggataa tgaactctta tcattataag gcggtaacct
     4741 cttttttatg tgattaactt tcattattac cacttctgtt tatcgattca gctctggaga
     4801 tacagaaaaa ttcagtgcag gtgtataaat catcaccatg cggaatacat aaaatatgtt
     4861 aatatttgtt ttccgcagga aaatcttaag tgtggacagg tatttatgat accatttaaa
     4921 atagattcag cactcactct ttccggacag ataatacatt tcaataccat ataattatat
     4981 aacaggaatt atatgaataa aatatacgcg cttaaatata gctcccttac tggtgggctt
     5041 atagctgtgt cagaattaag taagaaggtc acaggaaaaa ccgatagaag attaatgacg
     5101 gtttccctgg tattatcagt gactctttct gctttaccgg gtaaagcatc aacggtcagt
     5161 gcagaaatac catatcagac ttttcgtgac tttgctgaaa ataaaggtgt gtttaccccc
     5221 ggagtgacag gaattgaaat aaaagataat aatggaaatg cagttggtac gcttgatgtg
     5281 ccaatgattg acttcagcag cgtgtcacgg cggggctcac tgaccctgtt gtcgcaggga
     5341 tatggtgtat cagcaaaaca cggagacctt ggtgatgtaa acaatgccag ttttggctat
     5401 gataaaaata attatactgt agtaaagaac aacaaacatt cgggccttga tttttctctg
     5461 catcggttca gcaaactcat cacagaggct gcacctgctg atataaatat attcggacaa
     5521 cttagtgatt cgtcacagta tactgctttt tacagagcag gcgctggcac acagtatatt
     5581 aaagaacgct caggtaaaca gactcatatc ccgggaacat ttttaactgg tggaacagta
     5641 ggcactccat ggtattcagg aaataattta atatcaagct ctccaggtga cacctataat
     5701 aaatcacagg gaccattagc cagttatggg cagatgggtg acagcgggtc gccattattt
     5761 gcttatgatt ctctcagtga aaaatggtct ctggctggag tcaccctgca taactatggg
     5821 gtgaacggtt acagaaataa ctggttgtta ttacctgagg attatatcag aaatattatt
     5881 gtagccgatt tcgatcccat tatttcattt aataaaaaca gtaaagaaca tatgtcatgg
     5941 acatatgatg cagccaaagg cgtgggccgt attcagcagg atgaccaaca gtttgtgatg
     6001 catggtaacc tgaatggtaa tctgaatgca ggtaaaaatc tttattttac aggcgaaaat
     6061 ggcattattg atttaaaaga taatgttaat cagggtgccg gatatcttca gtttgctgat
     6121 gattatacag taacaacgag caatgacagt tcatggtccg gaggaggcat tattgtcaat
     6181 tatggtacaa ctgtaaaatg gggtataaac ggggttttcg gagatgacct tcataaagtt
     6241 ggggacggaa cgcttattat taatggaacc gggaaaaatg aagggggact aaagattggg
     6301 gctggtactg taatccttga acaaaaagcg aaaaacaacg attcaacggc tttttcttcc
     6361 attaacatca gtgggggaaa ctcgcgcgtt aaactttcag gagataatca gattatacct
     6421 gataatgttt cctggggatt ccggggggga tatttagata taaacggaaa agacactgag
     6481 ttctcccggt tacaggctgt tgattatggg gctgcaatta ttaacagcag catagacaag
     6541 tcattattga ctttaaatct gtcaccattg aaaaaagatg aaatagctgt aagcgtgaag
     6601 gtgttagata tgaacgccat ctttcagggg ggacacggga cagccggaga tttgtataaa
     6661 acaacttttt acggcccgac acagtattat ctgctgaaaa aacctaaatt tggcagtgtg
     6721 ctgatggggg cactgaaaaa caccagcgaa tggcaatttg caggaacaga cctgaatcag
     6781 gccgttgata tggcaaagaa taataaactg acatcaagtg cacaggcttc atatctttat
     6841 cacggtaagc tcctggggaa tatggatatt gtcattccgg agcttacagg caatgatatt
     6901 ttaacgcttg atggctctgt cagtatatca ggagatatgt caaaacagga cggtgctctt
     6961 atcttccagg gacacccggt tattcatgca gggcaaactg tttctgcatc gcagagtgac
     7021 tgggagaaca gggagttctc actcaacaat ctgaatctta ataatgcgga cttcagtctg
     7081 tcccgtaatg catttatgaa cgggaatatc agggccgtta accagagcac tgttattatc
     7141 ggcggagata cagtctttac tgataaaaat gacggaacag gtaatgatgt catcagtgtt
     7201 gaagggaaat ctgctgccgc aggaacatcc tcctatacag ggcatatcac tctggagcaa
     7261 aaatcagcac tggatatccg cgataatttt cgtggcgggg ttacgtctga agacagtcat
     7321 atcaatgttt cttcatcttc agtcctgttc tcagatgcat cgtcatttat aaacagctcc
     7381 ctgaatattc ataaaggagg tgcgctgacc gctcagggag ggctgtttac aagtggaagc
     7441 attgatattg gtgacgcttc ccttctgctt accggtaca
//
LOCUS       DAZGHF010000186         7421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGHF010000186 DAZGHF010000000
VERSION     DAZGHF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.187"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..686)
                     /locus_tag="ACHK3A_004507"
     CDS             complement(<1..686)
                     /locus_tag="ACHK3A_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO8742496.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPAHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKR"
     gene            complement(697..1698)
                     /gene="ltaE"
                     /locus_tag="ACHK3A_004508"
     CDS             complement(697..1698)
                     /gene="ltaE"
                     /locus_tag="ACHK3A_004508"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO8742497.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1735..3453)
                     /gene="poxB"
                     /locus_tag="ACHK3A_004509"
     CDS             complement(1735..3453)
                     /gene="poxB"
                     /locus_tag="ACHK3A_004509"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO8742498.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3586..4554)
                     /gene="hcr"
                     /locus_tag="ACHK3A_004510"
     CDS             complement(3586..4554)
                     /gene="hcr"
                     /locus_tag="ACHK3A_004510"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO8742499.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4566..6218)
                     /gene="hcp"
                     /locus_tag="ACHK3A_004511"
                     /gene_synonym="priS"
     CDS             complement(4566..6218)
                     /gene="hcp"
                     /locus_tag="ACHK3A_004511"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO8742500.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6362..7261)
                     /gene="lysO"
                     /locus_tag="ACHK3A_004512"
     CDS             complement(6362..7261)
                     /gene="lysO"
                     /locus_tag="ACHK3A_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO8742501.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         1798 a         2025 c         1933 g         1665 t
ORIGIN      
        1 cgcttaccgc tcaccgccat aaaatgttca aactgttgct gataactgag cacttctggt
       61 ccggcggctt cgaagatgcg atgttcgctg gccggatgat ctaacagcgc caccagatag
      121 tgcagcaagt tttccagcgc gatgggcgtg gtgcgtgaac gaacccagcg tggcggcgtt
      181 aacaccggca ggttgtagac catatcgcgc atgacttcga acgccgctga acctgcacca
      241 acgattattc cggcacgtag ttcggtcacc gggacacccg cttcacgaag aatatccgcc
      301 gtagcctgac gggcgcgcag atggtcagac tgctcatgtg ccggggcctg caacgaactg
      361 aggaagatta attgcttaac agggacttcg cgtagcgcat cgcggacgtt gagagccacc
      421 tggcgctcct gagcgataaa atcgccgcct tcgcccatgc tgtgcaccag aaaatagacc
      481 gtatcgatat cctgcaacag ggccggaagg ttatccggcc agctgagatc gactttatgg
      541 caactgacgt ttgccagttg cagctttgca agcctgtcga catgacgtgc cgccgccagg
      601 atctgatgcc cttgctggct gagagttcgc accagatgct gaccaatgta gccactggca
      661 ccgagaacta aaatgcgttg cggcacgtct ctctccttaa cgcgccagga acgcacgcca
      721 gtgggcggca acttccgcca gttgttcacg cgagacgtca agatgggtca ccaggcggac
      781 aatcggcgag gcgttaatca gcacgtttct cgctttcatg tattcgccta acgcggcagc
      841 attttcttcc ccgacgcgaa caaacagcat attggtgtcc tgacgcatca catccgcgcc
      901 tgcttcacgc aactgctccg ccatccaggc ggcgttatcg tggtcttcct gcaaacgtgc
      961 gacgttattt ttcagggcat acatcccggc ggcagccaga atgccggact gacgcatccc
     1021 gccacctgtc attttccgcc agcgaatggc acgtttaatg taatcacgat taccgacgag
     1081 taatgaaccg accggcgtcc caagaccttt cgacaggcaa atggtgaacg aatcacaata
     1141 ttgcgtgatc tctttcagtt cgcagccgta agccaccacg gcattaaaga tgcgcgcacc
     1201 gtcaacatgc agcgccagat tgcgctcgcg agtaaattcc catgcttctt tcaggtattc
     1261 ccgcggcaac actttgccgt tgtgggtgtt ttccagactg agtaatttgg tgcgggcgaa
     1321 atggatatcg tcgggtttga tcttcatcgc cactttatcc agaggtagcg tgccgtcggc
     1381 agccgcgtcg atgggttgcg gctgaatact gcccagcact gctgcgccac cggcttcaaa
     1441 cagatagtta tgcgcggcct gaccgacgat atactcttcg ccgcgttcgc agtgactgag
     1501 cagagcgacc aggttggcct gagtgccggt aggcagaaaa atggcggctt ctttaccgga
     1561 aagctctgct gcatagtcct gcagagcatt aacggtaggg tcgtctccgt aaacgtcgtc
     1621 cccaaccggg gcggccatca tcgcttcgag catggcgcgg ctcggtcggg taacggtatc
     1681 actgcgtaaa tcaatcatgg catgtcctta ttatgacggg aaatgccacc ctttttacct
     1741 tagccagttc gttttcgcca gctcgatcac ttcatcgccg cgtccgctga taattgcgcg
     1801 cagcatatac aggctgaaac ctttggcctg ttcgagtttg atctgcggtg gaatggctaa
     1861 ctcttctttg gcgaccacca catccaccaa caccggaccg tcgatggaga aggcgcgttg
     1921 cagggcttca tcgacttcag aggctttttc tacacgaata cccgtaatgc cgcacgcttc
     1981 ggcaatgcgg gcaaagttgg tgtcgtgcag ctcagtaccg tctgtcaggt agcctccggc
     2041 tttcatctcc atcgccacaa agcccagcac gctgttatta aagacgataa ttttcaccgg
     2101 cagtttcatc tgcactactg agaggaaatc gcccatcaac atgctaaaac cgccatcgcc
     2161 gcacatggcg accacctgac gttccggctc ggttgcctgc gcacccagcg cctgcggcat
     2221 ggcgttagcc atcgaaccgt ggttaaacga acctaacagg cgacgcttgc cgttcatttt
     2281 tagataacgt gccgcccaca ccgttggcgt accaacgtca caggtgaaaa tagcgtcatc
     2341 ggcggcaaaa tgactaattt gctgcgccag atattgcggg tgaatggctt tctcgctcgg
     2401 tttagctaaa tcgtccagcc ctttgcgggc gtcgcggtaa tcttccagcg ctttatccag
     2461 aaacttgcga tcggcttttt cttccaccaa tggcagcaat gcacgcagag tcgacttgat
     2521 atcgccgacc agtgccatat ccaccttgct gtgagcgccg atgctggctg ggttgatatc
     2581 aatctgaata attttggcat cggtcgggta gaacgcacgg tagggaaact gagtaccgag
     2641 cagcactaac gtgtcggcgt tcatcatggt atggaaacct gacgaaaagc cgattaatcc
     2701 cgtcattcca acatcatatg gattatcgta ttcgacatgt tctttaccgc gcagggcatg
     2761 gactataggc gctttaattt tcccggcaaa ctcaactaac tctttatgcg cccccgcaca
     2821 gccgctgcca cacatcaggg cgatattgct ggaataacgc agcagctgcg ccagtttgcg
     2881 taactcttct tcttccggtg ttactaccgg ctgtggcgca tgataccagt gggtagttgc
     2941 cccttctggc gcaggtttta acgccacgtc gcccggtaac acaacaacgg aaacgccacg
     3001 gttaagcacc gctttgcgca tagcaattgc cagcacttgt gggatctgct ctgggctgga
     3061 aaccagctcg caatagtgac tacattcgcg gaatagctct tgtgggtggg tttcctggaa
     3121 atagccgctg ccaatttcgc tggagggaat atgagcggca atcgccagta ccggaacgtg
     3181 attgcggtgg caatcgaaca ggccgttgat taagtgcagg ttgccgggac cgcacgatcc
     3241 ggcacagacc gccagttctc cgctaagttg tgcttcagcg ccagcggcaa atgccgccac
     3301 ttcttcatgc cgggtggaca tccactcgat ggtgcccatg cgattaagac tgtcactaag
     3361 accgttcaga gagtcgcccg tgactcccca gatgcgtttc acgcctgccg attcgagtgt
     3421 tttggcgata taagctgcaa ccgtttgttt catggttctc catctcctga atgtgataac
     3481 ggtaacaagt ttagttcatc tgacggaggg ggaagggatg ggagaaaaag gaggcactaa
     3541 cggttaaata gcccgatgaa aggaatatca tcgggcatca ggcgattatg cgagaaccaa
     3601 atccccctgc ggatggcagg agcaggccag tacataacct tcagcgattt cggcgtcggt
     3661 cagcgtcatt gtgctgctca ccgtatattc accggaaacc actttcgtct tacagcagcc
     3721 gcaaacacct gcgcggcagg cggcgacaac cggaacgtta ttgctttcca gcgcctccag
     3781 tagcgtggtg ccaaccgggg cgtaaaattc tcgtgccggt tgcagtttgg tgaatttcag
     3841 accgctggtc gccgcttccg ctactggggt gaagaatttc tctttaaaga aacgcgtcac
     3901 gccgagcgct ttcacttcct gctctaccca atccatatat ggagcagggc cacaggtcat
     3961 cacggtacgt gaggctaaat cagggacacc tgccagcagt tcgcgagtga gacgaccagc
     4021 gataaagcct tcggtaacgt tattttccgc caccagcgtt accggatagt tacgccactc
     4081 atcggcgaaa atcacatcct gcggcgtacg cacgttgtag atcacctgca catcggcctg
     4141 tggacggttc ttcgccagcc agcgacgcat cgacataatc ggcgtaacgc cgcagcctgc
     4201 cgccagcaac aggaatttat cttctgcttt atcatcgcag gtaaattccc ccatcgcgtc
     4261 cgaaagccag agataatcac cgcgttttac atcgcgcgtc agccactggg agccgacacc
     4321 gtcatcaatc cgccgcacag tcagggtgat gtattcactc acgcctggcg tggaggaaat
     4381 ggtgtaagca cgcagcgttt ccgctgagtt acgcacgctg accagtgcat attgcccggc
     4441 gcgatatggg tagtaatcgt ggcaaatcag ggaaatcgtc cacacatccg gcgtttcttg
     4501 cgtaatgtga tgaacctgca tccgccacgg gcattgattc gttggcatcg tcatcgacaa
     4561 actccttacg cgctcaacag ttgcttcatg tcttcttcaa cagtggtgat agaacgcagg
     4621 ccgaatttct cgttcagcac cgccagcagg tctggtgtca ggaaaccagg tgcagtcggg
     4681 ccggtgacga tatttttcac acccagagaa agcagcgtca gcagaatgac aatcgctttc
     4741 tgttcaaacc aggagagcac cagcgacagc ggcagatcgt tgactccgca gcccagtttc
     4801 tctgccagcg ttaccgccag aataattgct gagtaagcat cgttacattg accagcatct
     4861 accagacgcg gcagaccttc gatatcgcca aactcaagtt tattaaagcg atatttaccg
     4921 caagcgaggg tcaggatcag gcagtcatcc ggcacgctgg tggcgaaatc ggtgaaatag
     4981 tgacgctcgc cgcgtgcgcc gtcacagccg ccaagcagga agatatgacg cagtttttca
     5041 cggctcacca gatcaatcag cgtatcggct gcgccaagca gtgtctggcg accaaagccc
     5101 acggtgatca ggtgcggaat ttcgctgtac gggaagcccg ccatctgttg cgcctgggca
     5161 atgactgcag agaaatcttc gccatccaga tgacgcacac ccggccagcc aacgatgctg
     5221 cgggtccaga tacgatcgtc ataagcgcct acggttgggt cgatgatgca gttcgaggtc
     5281 atcacgatgg ggcctgggaa acgagcgaac tccacttgct gattctgcca gccgctgccg
     5341 tagttaccga ccagatgctt gaatttacgc aactccgggt agccatgcgc aggcagcatt
     5401 tcgccgtggg tgtaaacatt aacgcccgtg ccttcggtct gttccagcag gttgtagaga
     5461 tctttgagat cgtgaccgga aatcagaatg catttacccg ccgtcgcttt gacgttgacc
     5521 tgagttggcg tcgggtgacc gtatttaccg gtttcgcctg cgtccagaat gctcatcact
     5581 ttgaagttca tctggccgat ttccattgaa cactcaagaa gcgcgttcat atcggcaggc
     5641 caggtcccca gccacgccat gattttatgg tactgggcat aaatatcgtt gtcgtattga
     5701 ccgagaacat gcgcgtgttc catataggcc gccgcacctt tcaggccata caggcacagc
     5761 agacgcaggc cgagaatgtt ttcgccaatc gccgctttat ctttgttagg ggtaaattct
     5821 gctgcctgac gttgcagctc gccgagatcg tcgctcacca gttgcaggtc agccatcggg
     5881 ttatcgacgc gggcgttggc atctacagcc aggcattgtg ctttcagcgc ctcgcgcagg
     5941 gcaatcgctt cacgagcgta gccgacaata cgcggagaat cgaagttaac gttggtcagg
     6001 gttgagaaaa aggcacgtgg cgcaaagctg tctacatcgt ggttgataat gccgtattca
     6061 cgtgctttca ctgcccaggc cgaaagccct tgcagcgccg cgatgagtaa atcctgaagg
     6121 tcagaggttt ctgccgtttt accacacatc ccctgcgcgt atgagcagcc gtttcctgcc
     6181 ggagtacgga tagtttgttc acattgcaca caaaacatga tcacaccttt taaagttata
     6241 tttaatatac atgtttaagg ttaagacgct taacgcgggg ataaaagaga tttttaatgc
     6301 aactttaagg gagattgatt tagcgcaatt ttggcggcag agatctaccg ccagagaggt
     6361 attatgcaga gaaaaaggcg atgagaatcg gcactaacag actaagaata aaaccgtgaa
     6421 caatcgccgc cgggaccata tccagcccgc ctgtacgttg aagaacgggc agggtgaaat
     6481 ccatcgacgt cgcaccgcat aagcccagtg cagtagagcg gctacggcga atcagcccag
     6541 ggattaacat aatagcaatc agctcacggg ccagatcatt aaaaaacgcc gcgctcccga
     6601 ttaccggacc aaaagattcg gtcaataaaa taccggaaag agaataccag ccgaaaccgg
     6661 aggccattgc cagcgcggta ttgatgggaa gatcaagaat aaaggcgtta attaaaccac
     6721 caattaatga actggcaacc accaccacgg cgacaatcat tccccggcga ttaaggacaa
     6781 tctgctttaa ggtcatgcca ttattgcgca actgaatacc gacgaggaaa agtagcagaa
     6841 ttaaggtgta ttcactggct tcggtcgcgt gttgtaagaa agccagtcca ctcagaccaa
     6901 tggcaaaacc aatcactact acgccgcaca gttttagcga ctccagcgcc atcgcaatac
     6961 gcgacgggag tttttcttga tgatggtggt tgcgccatgg caggccgcgc tccagccaca
     7021 tcagggcggc aatattacac agtaaaataa cggtaatact gacggcagaa taatgcagaa
     7081 tcgccaacag gttactggcg aggttatcga gaaacgccag actgataccc ataaaaaaga
     7141 gaataaggta aaccatccag cttaatagct gattaataac tcttaatgca gctttttggc
     7201 gaagcggaat gaggtaaccc acaatcaggg gaaccagaat gattaacagc ccagaaaaca
     7261 tgaaaaccca gtccttgcaa agatgaagtc gaaatgcgcg atgacacact actgaaagcg
     7321 gaaggacgag taaagttgca attaaaagga aatgttatgc atcagaggga gatttttaat
     7381 tctatatgag tgtttagcat tgttgcgctc ctttcatgtg c
//
LOCUS       DAZGHF010000187         7315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGHF010000187 DAZGHF010000000
VERSION     DAZGHF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.188"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            155..1036
                     /gene="htpX"
                     /locus_tag="ACHK3A_004513"
     CDS             155..1036
                     /gene="htpX"
                     /locus_tag="ACHK3A_004513"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO8742502.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(1082..2455)
                     /locus_tag="ACHK3A_004514"
     CDS             complement(1082..2455)
                     /locus_tag="ACHK3A_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8742503.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            2632..3423
                     /gene="kdgR"
                     /locus_tag="ACHK3A_004515"
     CDS             2632..3423
                     /gene="kdgR"
                     /locus_tag="ACHK3A_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO8742504.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(3567..3806)
                     /locus_tag="ACHK3A_004516"
     CDS             complement(3567..3806)
                     /locus_tag="ACHK3A_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO8742505.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            3965..4108
                     /gene="mgrB"
                     /locus_tag="ACHK3A_004517"
     CDS             3965..4108
                     /gene="mgrB"
                     /locus_tag="ACHK3A_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO8742506.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            4183..4470
                     /locus_tag="ACHK3A_004518"
     CDS             4183..4470
                     /locus_tag="ACHK3A_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO8742507.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            5140..5283
                     /locus_tag="ACHK3A_004519"
     CDS             5140..5283
                     /locus_tag="ACHK3A_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO8742508.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            5296..5505
                     /gene="cspE"
                     /locus_tag="ACHK3A_004520"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             5296..5505
                     /gene="cspE"
                     /locus_tag="ACHK3A_004520"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO8742509.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            5671..6480
                     /gene="rlmA"
                     /locus_tag="ACHK3A_004521"
     CDS             5671..6480
                     /gene="rlmA"
                     /locus_tag="ACHK3A_004521"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO8742510.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(6477..7043)
                     /gene="mntP"
                     /locus_tag="ACHK3A_004522"
     CDS             complement(6477..7043)
                     /gene="mntP"
                     /locus_tag="ACHK3A_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO8742511.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDKEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     regulatory      complement(7175..7265)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT         1984 a         1644 c         1829 g         1858 t
ORIGIN      
        1 acagcagcaa gatgccgtaa atcagatgct acaaaatgta aagttgtgtc tttctggtga
       61 cttacgcact atccagactt gaaaatagtc gcgtaaccca tacgatgtgg gtatcgcata
      121 ttgcgttttg ttaaactgag gtaaaaagaa aattatgatg cgaatcgcgc tcttcctgct
      181 aacgaacctg gccgtaatgg tcgttttcgg gctggtactg agcctgacag ggatacagtc
      241 gagcagcgtt caggggctga tgatcatggc cttgctgttc ggttttggtg gttccttcgt
      301 ttcgcttctg atgtccaaat ggatggcatt acgatctgtt ggcggggaag tgatcgagca
      361 accgcgtaac gaaagggaac gttggctggt caatacggta gcaacccagg ctcgtcaggc
      421 ggggatcgct atgccgcaag tggctatcta ccatgcgccg gacatcaatg cttttgcaac
      481 cggtgcgcgc cgtgatgcct ctctggttgc tgtcagcacc ggtttgctgc agaacatgag
      541 cccggatgaa gccgaggcgg taattgctca cgaaatcagc cacatcgcca atggtgatat
      601 ggtcaccatg acgctgattc agggcgtggt gaacaccttc gttatcttta tttcccgtat
      661 tctggcgcag cttgccgcgg gttttatggg cggaaatcgt gatgaaggtg aagagagcaa
      721 cggcaacccg ctgatctact ttgcggttgc aacggttctg gaactggtgt ttggtattct
      781 ggcgagcatt atcaccatgt ggttctcgcg tcatcgtgaa ttccacgccg atgcgggttc
      841 agcaaaactg gttggtcgcg agaaaatgat tgctgcattg cagcgcctga aaaccagcta
      901 tgaaccgcaa gaagcaacca gcatgatggc tttctgcatt aacgggaagt cgaaatcgct
      961 cagtgagttg ttcatgactc acccgccact ggataaacgt attgaagctc tgcgtacggg
     1021 tgaatacctg aagtaatatg aagtaaatac taaaaagcgc gtcctgatcg acgcgctttt
     1081 tttatgccct ggatcgtggc tgagtgatac gtaaaccact gacacaggct gcaatcaccg
     1141 ccagaatagc cgcagctatc agcgagacgt gcgtaccatt atcaccaaac tgatttagca
     1201 tcagcgccac cagagccgcg ccgctactct gacccagaag acgagccgtc cctaacatgc
     1261 cactggctcc accgctacgt tcgcgaggag cggaggtaat aatggtgtgg ttattgggtg
     1321 actggaacaa gccaaatcca gcaccacata agatcatcgg ccagataata ttgatatccg
     1381 caggtgacgc tggcagcaga actagggaaa aaagccccgc agccatgatg aataacccta
     1441 aagcacccag caatcctgca tgtacgcgtt caatcaaata gcctgccagt ggagccatca
     1501 ccattgttgc taacggccac ggtgtcagaa gtaaacctgt ttcgacttca ctacgcccga
     1561 gcacggtttg caggtaaaag ggaagggaaa ccattgccag catttgtgcg cagaaagagc
     1621 aaacagatgt gcaaatagaa agtgaaaaca gcgggatacg cagtaaatcc accggtagca
     1681 gcggtacggg aagagaaagc tggcggcgaa taaagaaaat accaacgaca accattacca
     1741 ccagttccgc accaatcaat gtcagcgatt gcccctgagc gaaaccactc aatgcagtaa
     1801 taagcaggcc gaaggttaac gcgttcatca cggcgctggg caggtcgaaa cggggtttac
     1861 tggcgcgaga accattgggt ggcagaaaac gcatcgccag aagcagggcg ataataccca
     1921 acggtacgtt gattaaaaat aaccatttcc aggatgcgat ggagaggatt gctgcagcaa
     1981 ttgtcggccc ggcagcagaa gaaacggcaa caataaacga gtttatgccc atccctctac
     2041 ccagaaaacg ttgtggatag atcaggcgga taagtgcggt attaacgctc atcaacgctg
     2101 caccgccgaa accttgtatg acacgcgcaa gggtgagcat ttgcagcgaa tcagaaaggg
     2161 cgcagaatag tgaagacaac agaaaaacga ccagaccgca tttataaata cgtcgatagc
     2221 caaacatatc gcccagaaac gaaaacgaga gcagggagat gacaatggcg atttgatagg
     2281 cgttcactac ccagatggaa ctggctggcg tggcatgaag gtccgtggcg attgttggca
     2341 aggcgacgtt ggcgattgcg ccgtcaagga cagccatcga aataccaatc acaatggtta
     2401 atatcgcacc gtatcgctgg ggcaatggca ggccgtcggc ctgaactttt ggcataggaa
     2461 ttttatatct ttggtgaata atcaacaggg gtatgattct agcactgata ttcacggtgg
     2521 atgtcgcaga attgtaacta accggcaaaa gatggattgc gaccaccctg gtgcgaaatt
     2581 ataataaaaa acagttctga tttttataaa acactcgcaa tgaggtgata aatggctaac
     2641 gcagatctgg ataaacagcc tgattctgta tcttccgtgc taaaagtttt tggcattttg
     2701 caggcgctgg gtgaagagcg cgaaataggg ataaccgagt tgtcgcagcg cgtcatgatg
     2761 tcaaaaagca ccgtttatcg ctttttacag accatgaaaa ccttaggtta tgtggcgcag
     2821 gaaggggagt cggagaaata ttcgctgacc ctgaaattgt ttgaactggg cgctcgcgcg
     2881 ttacaaaacg tcgatttaat tcgtagcgca gatatccaga tgcgtgagat ctcccgcctg
     2941 accaaagaaa ctatccacct cggcgcactg gacgaagaca gtattgttta cattcacaaa
     3001 attgactcta tgtacaattt gcgcatgtat tcacggattg ggcgtcgtaa tccgctgtac
     3061 agcaccgcga ttggtaaggt actgctggca tggcgcgatc gcgatgaagt gaagcaaatt
     3121 cttgagggcg tggagtataa acgcagtacc gagcggacca tcaccagtac agaagcgtta
     3181 ttacccgttc tggaccaggt gcgcgagcag gggtatggcg aagataacga agagcaggaa
     3241 gaagggctgc gatgcattgc ggttccggta tttgatcgct ttggcgtggt cattgccggt
     3301 ttgagcatct ccttcccgac gttacgtttc tctgaagagc gtttacagga atatgtcgca
     3361 atgttgcata ccgcggcgcg caaaatttct gcccaaatgg gttatcacga ctatccgttc
     3421 tgatgagagt aagaacctgt ctgaatatca aacagacagg ttctttattt agcatgagaa
     3481 aaataaagtt gaaggtggcg ttatattaaa cgcacttgcg ataagaatat tttactcatg
     3541 agtgagaatc tggttattta ttacccttaa ccattatcga ccacaatatt gcttttacgt
     3601 aacaacgggc aatctgttat ccccagaaaa ccacttttag tgtgcaggta ttgtaccgtg
     3661 ctggtgcctc tggcggtcag ataggtacat tgcaaaccta atcccgcggc attctctttg
     3721 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
     3781 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
     3841 tagcggcaat atgcgctgaa gcaagcgact cattctgaaa aagcacgaat atcgacatag
     3901 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aaacggagat
     3961 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
     4021 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
     4081 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
     4141 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
     4201 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttctttt tatcgggctt
     4261 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
     4321 gaggcgttgc tggatcagca aaaacgtcaa aatgctctgt tacgtcgttt gtgcgaagca
     4381 aacgaaccag agaaagcaga taaaaagacc gttgagagtc aaaaatcggt tgaagacgaa
     4441 gatattattc gtctggtcgc cgaacgataa tctttcctca atcttgagta accccgtcgt
     4501 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcgtcaaatt
     4561 aaaactctgg tctgacttca aataattatc atattattta ttttcgtaac gggatatcag
     4621 aaaaagatat ttagcccatt aatttattag gatgtttaca tcggatttgt gattaagcgt
     4681 ggtattattt attagcgaaa cgtttctctc ttgatttttt tgcgcattca tcagtttttc
     4741 ttattttaaa tttacaatac cattggggat tgacttctct ttggggtaat tggtggccgt
     4801 taactgactg ttttatgaga aaaagtgata taacttttta ttcattgcat agcagaaaat
     4861 gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt
     4921 gaactatgtt agaaaatacg tcagtttaag tatctgcctg aactggcaag gttaagcaca
     4981 atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga
     5041 gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt
     5101 gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa
     5161 gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt
     5221 gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc
     5281 taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa
     5341 ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctctgct
     5401 atccagggta atggtttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag
     5461 gacggtcaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc
     5521 tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact
     5581 ttgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact
     5641 tgcggccttt agtatcctgc cggagttgtc atgtcgtttt cctgtccact ttgccatcag
     5701 cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg
     5761 aaagaagggt atgtcaatct gctgcccgtt cagcataaac ggtctcgtga tccgggcgac
     5821 agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg
     5881 cgtgatgcaa ttgtcgccca actgcgggaa cggcttgatg aaaaggccac agcggtgctg
     5941 gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgct gcccgaaatc
     6001 accacgtttg gcctggatgt ctcgaaggta gcgataaaag cggcggcgaa acgctatccg
     6061 caggtcactt tttgtgttgc ttccagccac cgtttgccgt tttccgatac tagtatggac
     6121 gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcgcgagt agtgaagcct
     6181 ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg
     6241 atttacaatg aagtacatct tcatgcacca catgcagaac aactggaagg ttttacatta
     6301 cagcagagtg atgagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta
     6361 ttgcagatga cgccgtttgc ctggcgtgcg aagccagaag tctggcaaac actggcagca
     6421 aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa
     6481 ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagcaccag cccgccgaga
     6541 atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtc
     6601 gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg
     6661 aaggcgagac caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc
     6721 agtagccaga aaccgtgccg acggcgcggc tctttatctt catcatctgc gccacgaaaa
     6781 ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtgattc
     6841 cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag cggtgtcagg
     6901 gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg
     6961 agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga
     7021 agaacagtag cagtgatatt catgacaacg tcctgaccgg ggtatccata aaacacatca
     7081 tcgcccccag caaacatgca tacatgaaca acaagccgcg atgggttttc gcgttcatgc
     7141 aggcatagcg attgatgtgc atatggtctc gcctgactga aagtgttcag tccgtacgtg
     7201 ccacgttttg caacgagtat gttgacacgt acatttccgg aatctggaaa tcggctactc
     7261 cccaaatgag ggcgcaacct taacatattt tgagaatata aaacaactgt tggaa
//
LOCUS       DAZGHF010000188         7301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGHF010000188 DAZGHF010000000
VERSION     DAZGHF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.189"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            83..1234
                     /gene="yiaY"
                     /locus_tag="ACHK3A_004523"
     CDS             83..1234
                     /gene="yiaY"
                     /locus_tag="ACHK3A_004523"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO8742512.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAEGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMVGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     NIPAGLRDLNVKEEDIPVLATNALKDACGFTNPIQATHEEIMAIYRAAM"
     gene            1364..2659
                     /locus_tag="ACHK3A_004524"
     CDS             1364..2659
                     /locus_tag="ACHK3A_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO8742513.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKHATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGH
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            2767..4305
                     /gene="aldB"
                     /locus_tag="ACHK3A_004525"
     CDS             2767..4305
                     /gene="aldB"
                     /locus_tag="ACHK3A_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO8742514.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4524..4667)
                     /locus_tag="ACHK3A_004526"
     CDS             complement(4524..4667)
                     /locus_tag="ACHK3A_004526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742515.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGTPMVIYGLVRPLTIE
                     TRD"
     gene            4850..5173
                     /gene="yiaW"
                     /locus_tag="ACHK3A_004527"
     CDS             4850..5173
                     /gene="yiaW"
                     /locus_tag="ACHK3A_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO8742516.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDVPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            5179..6315
                     /gene="yiaV"
                     /locus_tag="ACHK3A_004528"
     CDS             5179..6315
                     /gene="yiaV"
                     /locus_tag="ACHK3A_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8742517.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSAAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(6312..7286)
                     /gene="yiaU"
                     /locus_tag="ACHK3A_004529"
     CDS             complement(6312..7286)
                     /gene="yiaU"
                     /locus_tag="ACHK3A_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8742518.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAQPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1833 a         1733 c         1894 g         1841 t
ORIGIN      
        1 ataaacggta atgttgatca tattttcaaa tgaaaatcct cagtaagctg cccgcccttt
       61 tttacacttt caggagtgtg ttatggcagc ttcaaccttt tttattcctt ccgtgaatgt
      121 tattggtgct gattcattga atgatgcaat gaatatgatg gcagattatg gatttacccg
      181 taccttaatt gtcaccgaca acatgttaac gaaattgggt atggcgggtg atgtgcaaaa
      241 agcactggaa gaacgcaata tttttagcgt tatttatgat ggcacccaac ctaaccccac
      301 cacggaaaac gtcgccgaag gtttaaaatt acttaaagaa aataattgcg atagcgtgat
      361 ctccttaggc ggtggttctc cgcacgactg cgcaaaaggt attgcgctgg tggcagccaa
      421 tggcggcgat attcgcgatt acgaaggcgt tgaccgctct gcaaaaccgc agctgccgat
      481 gatcgccatc aataccacgg cgggtacggc ctctgaaatg acccgtttct gcatcatcac
      541 tgacgaagcg cgtcatatca aaatggcgat tgttgataaa catgtaactc cgttgctttc
      601 tgtcaatgac tcatctctga tggtcggtat gccgaagtca ctgaccgccg caacgggtat
      661 ggatgcctta acgcacgcta tcgaagcgta tgtttctatt gccgccacgc cgatcactga
      721 cgcttgcgca ctgaaagccg tgaccatgat tgccgaaaac ctgccgttag ccgttgaaga
      781 tggcagcaat gctaaagcgc gtgaagcaat ggcttatgcc cagttcctcg ctggcatggc
      841 attcaacaac gcatctcttg gttatgttca tgcgatggcg caccaactgg gcggtttcta
      901 caacctgcca catggtgtat gtaatgccgt tctgctgcca cacgttcagg tgttcaacag
      961 caaagtcgct gctgcgcgtc tgcgtgactg tgctgctgcc atgggcgtta acgtgacagg
     1021 caaaaatgac gcggaaggcg cagaagcgtg catcaatgcc atccgtgaac tggcgaagaa
     1081 agtgaatatc ccggcaggcc tgcgtgacct gaacgtgaaa gaagaagata ttccagtgct
     1141 ggcaaccaat gccctgaaag atgcgtgtgg gtttactaac ccgattcagg caactcacga
     1201 agaaattatg gcgatttatc gcgcagcgat gtaatcatca ttcccacaat ggctgtctaa
     1261 ttgtcagcca ttttttaaac tatctcaaaa tttgcgattt ttgagatagt ttctctttcc
     1321 tgctatacac tatctcaata ttgcgctttt ttgagataag cacatggcta tcaaatcacc
     1381 tcccggactt attcctttat cacacttatc gggtgaggaa ctcctggccc acctgcgctt
     1441 taaccgggtt accgatgaaa aaggacgtta tctgcctttt gatgagctgc agtaccgcat
     1501 caaaaaagga gaaaacgtcg atgtggcgtg gacgcttacc cgtctggcgc gcaacgcggc
     1561 gatccaacga attaattatt gcaatgaagc gggtgaacag gccggattca acattacgcc
     1621 ggtgattgca gaagcgtgcg agcttgttga taaacatgcc actgcactgg cgctgaaaga
     1681 tcagactgaa cggttacgcg gggcgggcgc cgagttaagt cagttacgcc tggaagagcc
     1741 gatcaccagt tcccaactgg aaggtgcgaa tactacaacg ctggtggcga gaaaaatgct
     1801 ggaaacaggg cggtcgccac gcactgaaga cgagcatatg attgcgggaa atgcccggct
     1861 gatggcggaa attccgcact tactcgcaga accgctgaca cctgcgctta ttcgccagtt
     1921 acatgccatt ggtatgggtg gaatcaatga cgcgaaatat cgtccgggag aatttcgtga
     1981 aacagatgat gttgtgatcg ccgactatga tggccatata gtgcatcaac cgcccgctgc
     2041 ggcattgtta ccggaacggc tggaaaaagt ttgccagtgg ttaaacagcc atgaagggta
     2101 tattcatccg ttggtccgtg cctgcattct gcattttatg ctggcccatg aacatccgtt
     2161 tcgcgatggc aacgggcgca ccagtcgggc gctgttttac tggtatatgc tgaaatcggg
     2221 ctacgatgtc tttaaatata tttccatcag ccgtttgctt catgctgcac cggtcaaata
     2281 tgccgccagc taccagtaca ctgaatcaga tggtatggat ctgacttatt tccttgaata
     2341 tcaggcgggt gtcattaaac gtgctctgca aaactggcag cagcatatcg atgaaattac
     2401 acaacgaagc gcaaaactcg atagtgtgct gtttagctct ggtgtgctta aacgcctgaa
     2461 tccgcgccag gtaacgctcc tgaatgtgat gctcgccaat ccggggaaag agtacacagt
     2521 tgctgaaatt agcgcgagct tgggcgtctc tgataatacg gctcgagctg atttacggac
     2581 aattgttaaa gaaggattcg cgcaagagaa aagaattaat aaccagcaag ccgtgtacgt
     2641 cgcacactac ccgctgtagc ttttgcccgt aaattcgtga tagccgtcgt aaagctgtta
     2701 ccgactggcg aagatttcgc cagtcacgtc tacccttgtt atacctcaca ccgcaaggag
     2761 acgatcatga ccaataatcc cccttcagca cagattaagc ccggcgagta tggtttcccc
     2821 ctcaaattaa aagcccgcta cgacaacttt atcggtggcg aatgggtagc gcctgccgat
     2881 ggcgagtatt accagaacct gacgccggtg accgggcagc tgctgtgcga agtggcgtct
     2941 tcgggcaaac gagacatcga tctggcgctg gatgctgcgc acaaagtgaa agataaatgg
     3001 gcgcacacct cggtgcagga tcgtgcggcg attctgttta agattgccga tcgaatggaa
     3061 caaaaccttg aactgttagc gaccgctgaa acctgggata acggcaaacc cattcgcgaa
     3121 accagtgctg ctgatgtgcc gctggcgatt gatcattttc gctattttgc ctcgtgtatt
     3181 cgggcgcagg aaggtgggat cagtgaagtt gatagcgaaa ccgtggccta tcatttccat
     3241 gagccgttag gcgtggtggg gcagattatt ccgtggaact tcccgctgct gatggcgagc
     3301 tggaaaatgg ctcccgcgct ggcggcgggc aactgtgtgg tgctgaaacc cgcacgtctt
     3361 accccgcttt ctgtactgct gctaatggaa atcgtcggtg atttactgcc gccgggcgtg
     3421 gtgaacgtgg tcaacggcgc aggtggggaa attggcgaat atctggcgac ctcgaaacgc
     3481 atcgccaaag tggcgtttac cggctcaacg gaagtgggcc aacaaattat gcaatacgcc
     3541 acgcaaaaca ttattccggt gacgctggag ctgggtggca aatcgccaaa tatcttcttt
     3601 gctgacgtaa tggatgaaga agatgccttt ttcgataaag cgctggaagg ctttgcactg
     3661 tttgccttta accagggcga agtttgcacc tgtccaagcc gtgctttagt gcaggagtct
     3721 atctacgaac gctttatgga acgcgccatc cgccgtgtcg aaagcattcg cagcggtaac
     3781 ccgctcgaca gcgtgacgca aatgggggcg caggtttctc acgggcaact ggaaaccatc
     3841 ctcaactaca ttgatatcgg taaaaaagag ggcgcagacg tgctcactgg cgggcggcgc
     3901 aagctgctgg aaggtgaact gaaagacggc tactacctcg aaccgacgat tttgtttggt
     3961 cagaacaata tgcgcgtgtt ccaggaggag atttttggcc cggtgctggc ggtgaccacc
     4021 ttcaaaacga tggaagaagc gctggagctg gcgaacgata cgcaatatgg cctgggcgcg
     4081 ggcgtctgga gccgcaacgg taatcttgcc tataagatgg ggcgcggcat acaggcaggg
     4141 cgcgtgtgga ccaattgtta tcacgcttac ccggcacatg cggcgtttgg tggctacaaa
     4201 cagtcgggca tcggacgcga aacccacaag atgatgcttg agcattacca gcaaaccaag
     4261 tgcctgctgg tgagctactc ggataaaccg ttggggctgt tctgatataa gaagctggtc
     4321 gcattgggta ttcattgcct gatgcgacgc ttacgcgtct tatcatgcct acggggacct
     4381 gaccgtaggc cggataaggc gtacacgccg catccggcat aaacaaagcg cacgttgtta
     4441 caatttacgt tttaaaccta cgtgcgcaat aacccagact acgtcaacag aagtgcgttt
     4501 ctggggcgtg ttttgccagg agtttaatct cgcgtttcga tggtgagcgg gcgaaccagg
     4561 ccataaatca ccattggcgt tccgaggcca atcagcaata aacggtagat aattgaaggt
     4621 ttaatacgct taatctgtac tgtggatgtt ttactggagt tattcattag tttggccaat
     4681 agttccatta tttcaatatg ataagcctcg cactgttgaa aagccaggct gcacacttca
     4741 gaaagtatat aaaaaacaat atgtcgttat ataaataagg aaattgcacc gcattatggt
     4801 atttcataat atgcgttaat tgttcttttc tatattctct gagggaaata tgttcctgga
     4861 ttattttgca ttgggagtgc ttatttttgt atttctggtg atattctatg ggattattat
     4921 tttacatgat gttccctacc tgattgccaa aaaacgtaat catcctcatg ccgacgccat
     4981 tcatgttgct ggttgggtga gtctttttac gttacatgtt atctggccat ttttgtggat
     5041 ttgggccacg ctttaccgcc cggagcgtgg atggggaatg caaaaccatg actcctccgt
     5101 tgtgcaacta caacagcgta ttgccgggct ggagaaacag ctcgctgaca tcaaatcctc
     5161 ttctgccgag taatacttat ggatttactg attattttaa cctatgtggc tttcgcatgg
     5221 gcaatgttta agatcttcaa aattcctgta aataaatgga ccattcccac agcggccctg
     5281 ggaggcatat ttattgtcag cggtctaatt ctgttaatga actataacca tccgtatacc
     5341 tttaaagcgc aaaaagcggt tatttctatt cctgttgtcc cacaggtgac aggcgtggtg
     5401 atcgaagtga cggacaagaa aaatacgctg attaaaaaag gtgaggtgct atttcgactg
     5461 gacccgacgc gttatcaggc tcgggtggat cggctgatgg cggatatcgt taccgcagaa
     5521 cataaacagc gggcgttggg cgcagagtta gatgagatgg cggcgaatac tcagcaggca
     5581 aaggccacgc gggataagtt cgctaaagag tatcagcgtt acgcacgtgg cagccaggcg
     5641 aaagtaaacc cgttttcaga acgcgatatc gatgtggcgc ggcaaaatta tctggcgcag
     5701 gaagcctccg taaaatcagc ggcggcggaa caaaaacaga tccagagcca gctggatagc
     5761 ctggtgttgg gtgaacattc tcaaatcgcc agcctgaaag cgcagctcgc ggaagcaaaa
     5821 tataaccttg agcagacgat agtgcgtgcg ccgagcgatg gttatgtgac ccaggtgctg
     5881 attcgtccgg gtacctatgc cgcgtcgctg ccgctacgtc cggtgatggt gtttataccc
     5941 gatcagaaac gacaaatcgt ggcgcagttc cgtcagaact ccttgctgcg cctggctccc
     6001 ggcgatgatg cggaagtggt atttaacgct ctgccaggca aagtgttcag cggtaagctg
     6061 gcagccatta gtccagctgt tcccggtgga gcttatcagt cgaccggcac cttacagacg
     6121 ttaaacacag cgccgggttc agatggcgtt atcgcgacca ttgaactgga tgagcatact
     6181 gatttgagcg cattaccaga cggtatttac gcccaggtgg cggtctattc tgatcatttc
     6241 agccatgtct cggtgatgcg caaagtactg ttacgcatga ccagctgggt gcattacctt
     6301 tatctcgatc attaatcttc gctttccgcc gcttgttcag gccgcagttg ctgcgactga
     6361 gcaagctggt ggcgaatttc acgcagcatt tcatccagca catactggac ccgccagggc
     6421 tggtattccc gtttacggaa gatagccacc agatccagcc accattcata cgggtgctga
     6481 aagcagggca ccagcgtgcc gtcttgcagg tcgctctgca cacttttgtc tggcgcaaac
     6541 acaatcccca ggtgatttcg tgccagctcc agcgcagatt gcgtgttgtc gcaaacgtaa
     6601 tttcccttca cgcgataatc ccgcacctct ttactcccgg cgacgttgaa gcgccagata
     6661 ttcgcgtcgt cgatcatcat cgagtcgatc aaaatacagg agtgatgttc gagttcgtca
     6721 ggacggctaa tgggatgttt ctcaagatag cgctggctgg cataagcggt tacggcatat
     6781 tgggtaataa aactggcaac cagcgattca tcttttggtt gggcgtagct gattaaaaca
     6841 tcacaatcat cgggaaattc gacgccttca taaaaggcat tacgctcaag attgcaggtt
     6901 tttagcgata aggtgatatc accgatatct ttaattttat cgattacatg tttggataaa
     6961 taggtaatta tgccggttgg tgcatagata gtgacccgac cacgtttctc atgcttataa
     7021 tctgcaataa aattattaag ctgctcgttt ctgtccagca tgtcgttgat atacggtaac
     7081 agcacggtgc caaaaggtgt gagcatcagc tgtcgggtgg ttcggtcaaa gacttttaaa
     7141 cccacttttg attcaaaatc agcaagatat ttgctgacgt tggcctgtgc gatgccaagt
     7201 acagtcgcgg catggctgat attttcactg gcagcgatta ccgagataat ttttaactcc
     7261 cggtacttaa gttgtaattt tgtcatcgca acagcccaat t
//
LOCUS       DAZGHF010000189         7211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGHF010000189 DAZGHF010000000
VERSION     DAZGHF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.190"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(239..3022)
                     /locus_tag="ACHK3A_004530"
     CDS             complement(239..3022)
                     /locus_tag="ACHK3A_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO8742519.1"
                     /translation="MRNLCFLLMLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPSNKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNAQDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLLPLITLYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLETRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3079..5451)
                     /locus_tag="ACHK3A_004531"
     CDS             complement(3079..5451)
                     /locus_tag="ACHK3A_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO8742520.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDFTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDVNPQ
                     IVFSYIKSKGNLSLNNGYEESNTDDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYINEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5489..7174)
                     /locus_tag="ACHK3A_004532"
     CDS             complement(5489..7174)
                     /locus_tag="ACHK3A_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO8742521.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAEYLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLSVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVI"
BASE COUNT         1881 a         1634 c         1605 g         2091 t
ORIGIN      
        1 cgaataatat tttttcattg aaaatcaata cattattgaa ttattgggtg gtagcaaggt
       61 gttttgtgtt gttattttta tgcactttct acgcctcctg aattatcacg taaaaatcag
      121 accttaaaat atcactatta gtacttggtt attattttga acgcatttat aaaattatta
      181 cataaaaata gcgaatattg ataaaatccc cgccaacgat gtgttgacgg ggctgttatt
      241 attttggcaa taatactccg gtataggtat tcaccggatg agaaagatat tgtttaacgg
      301 cagtgttaac attctctacc gtcatttgtt tcaataattg ctcctgctca gtccatgctg
      361 caggatcgtc atattgaata agactattta caatggtgtt cgctaattgt tgaacgctac
      421 gctgttggat atcgaggctg cgctgaacgt tttgctggta ttcgttcagt tcttgctcac
      481 tgatcccttt agccagacgc ttaaccatca cttcattcgc taacgttaac agttcatcat
      541 gtcgttccgg ttgacaagta aaagccagca aatgactgat atctttggcc tgaggatcaa
      601 ccgagaggcg agaagaaacg ctgtatgccc cagaggcctg ttcacgaata ttaacgcgta
      661 gatcttttgc cagtgcgacg ttaaaagcat cgagcgccat acgcgtctcc agattaaccg
      721 gtgtccggga atcataacgc ttccactgtg aaacctgtgc cacaggttca ttttgttctt
      781 ttacagtaac cgatgcgttg tccgtcgcgc gagttaatgg tttacctgcg gctaatggcg
      841 aatcagagtg tttgattgat cctaagtaaa gcgtaattaa cggcaggagt ttgtcttctg
      901 cgacattacc gacaatgaca aacgtgatat ccgctggaga tgaaaacaac tggcgatcgg
      961 cagccagcgc atctgcggca gtaaactgtg caatctgatt ttcttgcagt aatttcgtgc
     1021 ggtcatcagc atagcgcgtc tcatacatct gctgggcgaa tttctccgct ggacgctggt
     1081 cgagcgtttt caacgcctga atttgagcat tttgtagcga tgcccaaata ttatcgttaa
     1141 tcgtgctgtg ggtgattcgc tggttaatca actggaaacc aggttcaggg ttattagtcc
     1201 gcgcgctaac agagagcaat gtattcatgc cactgacttt actgctcatg gttaccgagt
     1261 tttccgcact ccagcgtttc aggctggaag aggagagttc accaacgccg cttccgctta
     1321 ctgctttatt tgccagcgcg ataagtgatt tttgctgcgc agggaaactt aaatcgcctt
     1381 tattagatac ggcagtaatt tgtagctttt gctcttcacc cgcggatttt gccagaataa
     1441 ccctggcacc attggaaagt gttaatgatg tcagattctc agccagcgtt tctttgctgc
     1501 taatttccgc ctgtgggtca gcgtctactg ttaacgataa atttctgcct gggaagatgt
     1561 aagccgccag ctttttgttg gcgtactctt tttccagcgc caggatagcc gcaggagaca
     1621 atgctttttt ggcggcaact tcattgttta ccatttgctc ccaaaatgcg tcctggttct
     1681 ttcttaactg ctgccatttt tccgccagac tttgcacggt aatttgctgc cacagacgtt
     1741 tcgaaagttg atatgtctct tccggcgaca agaacggcgt attatttaat gagctggatg
     1801 ccaggcgact ggtcagcata cgtaaatcac gctcagcttg ctgatcaacc gcatttttca
     1861 gccaggtaag gcgggtagat ttaacatcat cgagttcttc agcagaaaag ccatgctgat
     1921 caatggttgc caactctgcc attaatgcat tcgcagcatc ctgcatatta tcgtcttgtg
     1981 catttacacg gaaaaacaaa gactgataat cgggtgcaat tttgacgctg cgcgcagtgc
     2041 cgccagaaat agtcttcaac tcgcccgact gtatgcgttc ttgcagacgt tggttgaaca
     2101 gctgaaccaa catgctccat tcagcttgtt cgataaagct ttgctcatcg ttcacttgta
     2161 ccattggcag gcgataatag agtgcgatgc cgttcacccg gttttcttta tcattgatga
     2221 tattaaagcg caggtggttt tcggcttttg tcggccagac gcgattttca gctgctttgt
     2281 tagacggaag cttacttaaa ttatccttaa tcagcgccag cgcttcttta ctgtcaatat
     2341 cgccgaccac gataaaggtc atattatttg gttgatacca gcgttgataa aattggcgca
     2401 attgtgccgg tgtgaccgta gcgacagtat ccatcaggcc gataggttca cggtctaaat
     2461 tacgggtatt tgccagcagg aaagggcggc gcgcctgaga ggtgcgccat ttcgcatcct
     2521 gatgggcacg ccattcctca gtaattacgc cgcgttcagc gtctacttcg agtttttcaa
     2581 aggttgcggc attactccat tcactgaaga ttgccatcac ttgttgcaga ttttgtttct
     2641 gcgtagtcgg caaactcacc tgatacactg tttcgtcata gctggtatag gcattaacat
     2701 cgcgaccaaa acgcaggccc attgactcaa atgtttcgat gactttatta cccggccatg
     2761 tttttgtgcc gttaaacatc atatgttcta caaaatgagc cacgccgcgc tcattgtcct
     2821 cttcctgcaa tgaaccggta tgaatttgca gccataaatt tacctgatcc tttggttgag
     2881 catgcggata aatcatatat cgcaagccat tgtccagttg cccggtaatt aacttttcat
     2941 cctgcggtaa ggcggcggca atcagtcgcc cggggagcaa cagagttgcc actaacatca
     3001 gtaagaaaca gaggtttctc ataattatct ccatgcgaaa accgggcaaa tttacccggt
     3061 aaagtaaaat ccgaactatt aaaatttcat gctgacatca agccagaaag tacggccact
     3121 ggcgtaggtc gccacgccgg tattcgttgt atcaaccgcg gttttgctat ccagtacatt
     3181 gagaatatcg gcactgatcg tcaggttttg ttgtttcaga aattgtggcg tccaggacaa
     3241 gcgggtgtcc caggtcaggc tgctatccag tttctcgtca acatactgct tgtaatctga
     3301 atattcgttg atgtattgcg cattcgtctt acccaggata atgcgagctt tacgcgcttc
     3361 ttgccaggtc agcgtattcg cccacaccaa cccgctcggt tgatgcgtga aatccatgtt
     3421 taaggagatc tttaatggat tattaaaatc cgccactgga acgctatcgt aagagaccag
     3481 attaccgtta taaaccaccc ggttatcatc ggtattgctt tcctcataac cattgtttaa
     3541 cgacaagttg cccttgctct tgatatagct aaagacaatt tgcgggttaa catctacctg
     3601 gctgatatgc aggggttcgg ccagttcaaa actgaggttg aacgaatgcg ttttggtttt
     3661 gccgtcgttg ttatattcag taatggtggt tttagtcgcg ctgtcggtac gactgctttt
     3721 gctgatttga tcatgcgctt cacggtaaac atagtttgcg cgcgcaataa cgttcttacc
     3781 gattttctgc tgcaacccca ttgccagttc atcgttataa ggcgttttca aatcctgata
     3841 acgcgtcagg gttttattgc ctgataccga ttccgtccag ctattgcgga tatcacgtaa
     3901 tcccatatca agaatattcc cgccatagta acggttataa cctgcggtaa tcattgaggt
     3961 ttgatcagca aaaatatccc attccgtcat aaagcgcggg gagatattgt ggtttgacag
     4021 atagttgtca tagtcatacc gcacaccggg cattaacgac acattccgcc agctaatgcg
     4081 atccgccata tacagcgtgt agttgtcaat tcccagactg cctttacctt tatggtaaat
     4141 ggtatggtta gttttctttc ctgcagcatt aatcacatag gattcagact ggttatggcg
     4201 ttcagtccat gcatcggaat agatgtattc cgcgccgaag tagggttgat gcgaaacatc
     4261 acccacggcg aatttttgcc agtccaggcg tgttttgaag gtgtaattat ctacagcctg
     4321 ggaaatgtgt cctaatccac cacgggtgca acgacctgaa atatcaccat atgtacatga
     4381 aagttcggtg taccagatgt catgatcgtg acgggtataa tcactaatat gatcccaacc
     4441 aacggtagta cgtagtttgg cccatgcgag ctgcgtatcc atatcccatg ccagaccgta
     4501 ggatttatta cccatttcgc gatcagactg cgggaaggtg ctggtattat aatcacgact
     4561 ggagccggta tattttaagg taaaatcgtg ggtaaagcgg tcgctggcaa accaagtaaa
     4621 tttgctcaat gcggtatcga taacgttttt atactgtgcc cgaccggcga caatgccgtc
     4681 attcgaaaca taatccgcgc gggtgatatc agactggcgg cgcgataaac cggcggtaac
     4741 gccaaagtta tcagcgagtt cctgattaaa cgacaaggta taaaagttct ttttaaaatc
     4801 tggagagtaa taagtacttc ctgaagaacc ttgattaaat gcgctcttgt tattctcatc
     4861 gatatgcgat gttaaccagt ccgaacgcgt agtgcgataa cccagtttca cgctgctatc
     4921 atcagcgttg aagcgtttga tctttgcatc aattaccccg ccattgaagc gaccgaattc
     4981 aaccggcaca aaactgtcat aaagcgtcac attgtccagt aagctgacat caagataata
     5041 gccctgtgac atcccgctaa tattggttgc actactggca tcggactcat tcgctgggtt
     5101 caggttatta gttgcactaa taccatcaat caaataggca ttctggtagg gcgacgcacc
     5161 gtgaatagag attttctccg ggcgaatatc tccctggttc aatgaggtac tttgcgttga
     5221 atccatgcgc accgcagggt tggttctcag cagatcgctg atattgccat tcccggtcgg
     5281 taaacgctcg atgctttcac tggtgtaatg cgtgttgccg tttacgggta cagggacagg
     5341 agcatagacc gtcatctctt caaaaacatg catgtacatg tttttgtcat cgacggcctg
     5401 cgctacatca gcgccacata aaatgacgcc aggaataaga actcgcttca tttatttata
     5461 tctccgtaca gcagggcttt tattgttttt atataaccgc gctaatgtca caaatatcat
     5521 cagccaggtt ccagacaccg ggttgatgtg taaccataat gacgccgctt gtgggtagtt
     5581 tttcacgcac taaacgcagt aagcggatag cctcttgttc ctcaagatga gaggtagttt
     5641 cgtcaagaaa tatccatttc ggacgtcgta aaattaatcg tgccagggcg atacgttgtt
     5701 tttcgccgct ggaaagaata tcgccccagc gatcgtggtc atgaatacgc gcagccaatt
     5761 tccccagacc aacctgatgc agtacttcgc tcaacgattt atcgtctacg gacaggggaa
     5821 gtgctttaca aattatctct ttcagtaagc cggttttgat taacggtgtt tgtgacacat
     5881 accaactgtc agcaggagaa gaaatatcac ctttaaacca cggccagcag tgggataatg
     5941 ttttaagcag tgtggttttt cccgcaccag agtaaccttt cagtaatagc catttgcctg
     6001 gcgaaacatg aaagttcagg ttctctaata tgatcttatt atcaggagta cgaatactcg
     6061 cgtcagccac ttgtaccgca tgttggcaat ttttaggctt attcgtaggg cgctgttcgg
     6121 tgagttgatg gaactcatac aaacgatcga taaccgcagc cagttcagca agttcgtcat
     6181 atttataaat aaaccagctt aaattgttcg ataccagcat aaatgcctgg cgcgatttca
     6241 tcagtccgcc cagattgatc tgtccgctaa taaactgcgg caataacaga aagtagggaa
     6301 gaacgctaag cgagcgcgaa taaatattct gccagtaatc aagccaccgc tgacgattca
     6361 ttaaacgatg ccagttctct ttaatggtat gaaaattgtc gcttaactcc tggcgttgta
     6421 gatactcagc attgcttagc gcaatcagtt ctgcctgctt attatgctgc acaagattag
     6481 ttcgaaatgt cgcttcgcta cgttgttttt ccacattaag cggacgaata cgtttaccaa
     6541 ccttatgggt aaataaagtt ccaccgatca caatgagcac aacggtatag accatatacc
     6601 cctggatatt ccattctgtt ccaccaacag tgaatgagag cgtacccgcg ctttgccaaa
     6661 gaataacggt aaaggtgatc aacatgctaa gtgactggat gaagccaaat gaaaggctga
     6721 gcgttttact gatcagtaag agaatgtcct cagcgatacg ttggtcaggg ttatctgtat
     6781 ttttatgctc accgtagatc tgcgtgaagt agtagttttt atctgcaaac cagcgattaa
     6841 ggtagtaatc tgttaaccat tcgcgccagc gaatagtcag tagtttaatt agccatgttt
     6901 tgtttacgga tattaataca aagatcccca gcagggcagg gaaccaaagg acaagctgcc
     6961 aaagcttatc ggtttctttc tggcttagcg cattgaaaaa atcattattc cagtcgttta
     7021 accagacctg aattttaacc acgccaagga tcatggcgag gataataatg attaacaaca
     7081 ctgaagtttt attattctta cgcagccaaa atggcttcag cagacagaga tactttgcta
     7141 tcaacataca aaacgtaatg ggaatggtta tcattagcga aaattgattg tgccatccaa
     7201 tgattatcta t
//
LOCUS       DAZGHF010000190         7069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGHF010000190 DAZGHF010000000
VERSION     DAZGHF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.191"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..92)
                     /locus_tag="ACHK3A_004533"
     CDS             complement(<1..92)
                     /locus_tag="ACHK3A_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742522.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKR"
     gene            complement(303..1700)
                     /gene="ccp"
                     /locus_tag="ACHK3A_004534"
     CDS             complement(303..1700)
                     /gene="ccp"
                     /locus_tag="ACHK3A_004534"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO8742523.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2105..3754
                     /gene="treF"
                     /locus_tag="ACHK3A_004535"
     CDS             2105..3754
                     /gene="treF"
                     /locus_tag="ACHK3A_004535"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO8742524.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKACARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3805..4407)
                     /gene="yhjB"
                     /locus_tag="ACHK3A_004536"
     CDS             complement(3805..4407)
                     /gene="yhjB"
                     /locus_tag="ACHK3A_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO8742525.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4927..5826
                     /gene="rcdB"
                     /locus_tag="ACHK3A_004537"
     CDS             4927..5826
                     /gene="rcdB"
                     /locus_tag="ACHK3A_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8742526.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5875..6888
                     /gene="yhjD"
                     /locus_tag="ACHK3A_004538"
     CDS             5875..6888
                     /gene="yhjD"
                     /locus_tag="ACHK3A_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO8742527.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
BASE COUNT         1810 a         1810 c         1661 g         1788 t
ORIGIN      
        1 cgttttgact ccgcgatagt agaaatggcc tttgcaccaa aacgtgaatc gagtagttct
       61 gagcggaaat ccgttaacag cttctggtcc atttcgaact ccttaaattt atttgaaggc
      121 aataaaaaag taggatttat ccgcaatgga agcaaggcat tacaggctta atttaaataa
      181 caaaatccta agcagaaaaa cgacctttta taatataaac atttaatatg ataatattaa
      241 aaacagactt tattatcgta ataaaaatcg cccaaacact cctgttcggg cgatcaaatt
      301 aattattgtt tatcctgcat atacggcgtg taaaccccgt tcagactgtg caggaaagct
      361 acgatatcat ccacatcctc ctgcggcagc tctttgccta cctgatagcg cagcatcagt
      421 ttcaccgccc cgtccagcgt cggcacgtca ccgcgatgga agtacggtgc cgttaaagca
      481 acgttacgta aaccgggtac tttctgacgc aatttatcac gctcttcttt agtaacattc
      541 atacgaccaa tatccgccgc cgtaatttca ccaaagttaa agtctttttt cagccccaac
      601 ggttcaaagg aacgtccgcc gagaataata ccaccatgac aagttgcaca tttattatct
      661 ttaaataatt gatagccttt tttctgttgc gccgtcagag cattctcatc tccacgcaac
      721 catttatcaa atggggaatc cggcgtaatt aatgttttct caaattcagc aatggcatca
      781 gtaatatttt cgccactgaa accttgtgga tagacttcga ggaactgcgc tttaagctgc
      841 ggatcttttt ccagcttagc aataatttcg tcccaggatt tcgacgccat ttcaatcggg
      901 ttcaacggcg gtccaccagc ctgatcctgc aatgttgccg cacgaccatc ccagaactgc
      961 tcaacgttaa atactgagtt aaataccgtc ggcgcgttaa tcggcccaac tgcgccacca
     1021 acaccaatcg atgtttttct gccatcgacg ccccccgcat tcaacgcatg gcaatgagcg
     1081 catgaaatgg tgctatcagc cgataaacgg ggatcgtgat acagcgcaaa acccaacgcc
     1141 actttttgcg catcggtagg cagtttttgc gggatgggct gcaccggttc attgcgatgc
     1201 tccggagcag tatcattgct ggcgtaatat tccgcgcgct gttttgcaat ccaggccagt
     1261 atttccgccc gctcttcatc actcacctta cccgcccagt gtagcgcggt ataacgcgtt
     1321 ggcggcatag tttcatactg catcacccat tcaatcttat tcagatcgct ttgcgaaacg
     1381 ggtttattag ccagcagagc cgcacgcacg gcctcgaggt taaaagattt atatccaagc
     1441 tttatgtcgt aatccatcaa ctgtttcgcg ccgggaatat aataataggc gggtaattct
     1501 gccgaaggtg tatggcaata gtcgcatcct ttttcacgga gaaagcctaa aaccttatta
     1561 ttttcactga cagcagatgc ctgaacatcg gcctgtttac tgcgtttatt atcgtggtac
     1621 cacacataac cagataaccc taaatagcaa atcgcgacgc cagccaggcc gatcgcggta
     1681 atacgtgaga ccattttcat tattcttccc tcacggtggt gagttattgt gacccagaaa
     1741 attttccaga atgctgcaaa cgtctctgcg agacaaagcc atcataaaaa acagggagat
     1801 atgctttttg ataggaatca atcaattaca tgaatataaa ctatcaataa gatagccagt
     1861 gcctattacc cagatcagga atggcatatc atgattgcgg cgagagcagg attgaatgtt
     1921 ctgaaaatga aaaacaacac gaaatccgta tttttataaa ttccattgat attagtgcgt
     1981 tatatttttt ggcaggttaa taaacgtgat ccaccgcacg ctttgtcgcc caccaggcgg
     2041 agcgaatgac tacccttaaa gaaaagcccg ataattagcg acgaatttcg gaggtcggat
     2101 ccttatgctc aatcagaaaa ttcaaaaccc taatccagac gaactgatga tcgaggtcga
     2161 tctctgctat gaactggacc catatgaatt aaaactggat gagatgatcg aggcagaacc
     2221 ggaacccgag atgattgaag ggctgcccgc ctctgatgcg ctgacgcctg ccgatcgcta
     2281 tctcgaactg ttcgaacatg ttcagtcggc gaaaattttc cccgacagta aaacctttcc
     2341 cgactgcgca cctaaaatgg acccgctgga tatcttaatc cgctaccgta aagtgcgccg
     2401 tcatcgtgat tttgacttgc gcaagtttgt tgaaaaccac ttctggctgc cggaggtcta
     2461 ctccagcgag tatgtatcgg acccgcaaaa ttccctgaaa gagcatatcg accagctgtg
     2521 gccggtgcta acccgcgaac cgcaggatca cattccgtgg tcttctctgc tggcgctgcc
     2581 gcagtcatat attgtcccgg gcggccgttt tagcgaaacc tactattggg attcctattt
     2641 caccatgctg gggctggcgg aaagtggtcg ggaagatttg ctgaaatgca tggccgataa
     2701 cttcgcctgg atgatcgaaa actacggtca catccccaac ggcaaccgca cctattattt
     2761 gagccgctcg caaccaccgg tttttgcgct gatggtggag ttgtttgaag aagatggtgt
     2821 acgcggtgcg cgccgctatc tcgaccacct gaaaatggaa tatgccttct ggatggacgg
     2881 tgcagaatcg ttgatcccta atcaggccta tcgccatgtt gtgcggatgc cggacggatc
     2941 gctgctcaac cgttactggg acgatcgcga cacaccgcgt gacgaatcct ggcttgagga
     3001 cgttgaaacc gcgaaacatt ctggtcgccc gcccaacgag gtgtaccgcg atttacgcgc
     3061 gggggcggcc tccggttggg attactcttc ccgctggctg cgtgatactg gtcgtctggc
     3121 gagcattcgt accacccagt tcatccccat cgatctgaat gccttcctgt ttaaactgga
     3181 gagcgccatc gccaacatct cggcgctgaa aggcgagaaa gagacagaag cactgttccg
     3241 ccagaaggcc tgtgcccgtc gcgatgcggt aaaccgttac ctctgggatg atgaaaacgg
     3301 tatctaccgc gattacgact ggcgacgcga gcaactggcg ctgttttccg ctgccgccat
     3361 tgtgccgctc tatgtcggta tggcgaacca tgaacaggcc gatcgtctgg caaacgccgt
     3421 gcgcagccga ttactgacac ctggcgggat tctggcaagc gagtacgaaa ccggtgaaca
     3481 gtgggataaa cccaacggct gggcaccgtt acaatggatg gcgattcagg gatttaaaat
     3541 gtatggcgat gaccttctgg gtgatgaaat cgcgcgcagc tggctgaaga cggtgaatca
     3601 gttctatctg gaacagcaca aactgatcga gaaataccat attgccgatg gtgttccccg
     3661 cgaaggcggc ggtggcgagt atccgttgca ggatgggttt ggctggacta acggtgtggt
     3721 gcgccgttta attggtttgt acggtgaacc ataatatttt tacagccagc cgctaacttc
     3781 ctgctggctg taaaattatc ctcttcagga ggagatattt aacatcattg ccgcctgggt
     3841 gcgatttttc acttccagac ggcgatacag ggattccaga tgcgctttta ccgttccggt
     3901 actgatattc agcgccctgc cgatctcctt atttgattcg cccgccgcta acatggttaa
     3961 aatctcccgc tggcgggcgc ttaacgattt gagatcttta atgtcctttt ccggcgtcgt
     4021 ccgccagtct ccaggcagaa acatcatccc catcgccgca ctatttaccg ccaacgcaaa
     4081 tgtctcgacg gttgaatcac gaggcacaat ggccagcaca ttaaaatgga taacttcctg
     4141 taaccaccgt ttattgcaat ccgtcgccgt aattaacacc ttaacctcag gaaattgcac
     4201 cacggttttt tgcagcaacc agtaacaaaa ctcaccatcc tgatcgccat cgagcatcac
     4261 taaggcttca gggtaacttt ccagcttttg ccataactcg tctgcctgac tggccccctg
     4321 aatactcact cctggaatac gttgctgtaa actgattttc attccatgaa taaatattga
     4381 ctgcctgtca aacatgacta tttgcataac tgaatctcca cctgaatacg ttaaaaagac
     4441 ttaagtagtg gaagggtatt acccgcgaga aaaaataaga attcgccatt tggcggtggc
     4501 cattctacag agatgacgcg tagaaaatag ttaccgatat aaatagttac agctaaacgc
     4561 ctgaaattac atgtcgaggg cactatttaa aacaattttg aggatttcct tatattagtg
     4621 gttagtacgc atgcaattaa aaatgaaatt ccgcgaccac aagccaaaat aacaaatggc
     4681 aatgagacaa aaataagcac aaatagccaa tacgtcctct attcacttta aagggaatcg
     4741 ctgaaaaata cgctctgttt aaggggattc acctttctaa gaaagctatt cctccctttt
     4801 cctactgaga aatcgccaca ttcgtcatga caacattgtg aaacccggct ttagatgtta
     4861 gaaaaaacaa taaacaatgc gatatgcgcg ttactgtttc gctgtgaaca acaatcggtc
     4921 aaagaaatgg ataaaattca cgcaatgcag ttgttcatca aagtcgcgga gctggaaagt
     4981 ttttcccgcg cggcggattt ctttgctttg ccaaagggaa gtgtttcgcg ccagatacag
     5041 gcactggaac atcaacttgg cacccagctt ctccagcgca ctacgcgacg ggtcaaactc
     5101 acgccagaag gcatgaccta ttatcaacgg gcaaaagatg tgttgagtaa tctcagcgaa
     5161 ctggacggtc tgtttcaaca ggatgccacc agtatcagcg gtaaattacg catcgacatc
     5221 ccgccaggaa tcgcgaaaag cctgttactg ccgcgcttgt cggaatttct ctatctgcat
     5281 ccgggaattg agctggaact gagtagccat gaccgtccgg tagatattct tcatgatggt
     5341 tttgattgcg tgatacgcac tggcgcatta ccggaagatg gcgttatcgc ccgttctctc
     5401 ggcaaactga ccatggtcaa ctgtgccagt ccacactatc tgacgcgctt tggttatccg
     5461 caaagccccg atgatctgac ttcacacgca atagtgcgtt atacaccgca cctgggtgta
     5521 catccgttag gttttgaggt tgccagcgtt aatggcgtcc agtggtttaa gtctggcggc
     5581 atgttgacgg taaacagtcg cgaaaactat ctcgccgccg gtcttgccgg tctggggatt
     5641 attcagatcc cgcgcattgc cgtgcgcgaa gccctgcgtg ccgggcggct tattgaagtg
     5701 ttgcctggct accgtgccga gccgctctcc ctttcactgg tttatccgca gcgtcgggag
     5761 ctttcccggc gtgtaaacct gtttatgcag tggctggctg gcgtaatgaa agagcacctg
     5821 gactgaccga ctatactttt taagaatgac cacgacagaa ggacaaaaga gcggatgacg
     5881 caggaaaacg agatcaaacg tcccacccag gatctggagc acgagccgat taagcagttg
     5941 gataatagcg agaaaggcgg caaagttagc caggcgctgg aaaccgtcac cactactgcc
     6001 gagaaagtcc aacgccaacc ggttattgcg cacctgattc gcgcaacaga gcgctttaac
     6061 gatcggctgg gtaaccagtt tggtgcggct atcacctatt tctcattttt gtcgatgatc
     6121 ccgattttga tggtatcgtt tgccgcaggg ggctttgtgc tggcctccca tccgatgttg
     6181 ctacaggata tcttcgacaa aattctgcaa aacatcagcg atccgacgct agccgccacg
     6241 ttgaaaaaca ccatcaacac cgccgttcag cagcgtacta ccgtagggct tgtcggcctg
     6301 gcggtggcgc tttattccgg catcaactgg atgggtaacc tgcgtgaagc gattcgcgcc
     6361 cagtcacgcg atgtctggga acgctcgccg caagatcagg agaagttctg ggtaaaatat
     6421 ctgcgtgatt ttatttcgct gattggtttg ttgattgcgc tgattgtgac gctttcgatc
     6481 acctcggttg ccggttcggc gcagcaaatg attattagcg ccctgcacct gaacagcatt
     6541 gagtggctga aaccgacgtg gcggttgatt ggcctggcga tttccatctt cgccaactat
     6601 ctgcttttct tttggatctt ctggcgcttg ccgcgccacc gtccgcgcaa aaaagccctg
     6661 attcgcggaa catttctggc tgcgattggt tttgaagtga ttaaaatcgt gatgacctac
     6721 accctgccat cgctgatgaa atccccctct ggcgcagcat ttggttccgt gctgggactg
     6781 atggcatttt tctacttctt cgcccgtttg acgctgtttt gcgcggcgtg gattgccacc
     6841 gccgaatata aagacgaccc gcgaatgccg gggaaaacgc agccttaaaa taggccggat
     6901 gcggcgtacg aatttattcg gcctggctcc ccgtaggccg gataagatgc gccagcatcg
     6961 catccggcta taatgcgcac ataacctctt gaaactcttt cccagagcct ctttagccat
     7021 ctattcagga gcaaacaatt tcataccaac tcataacccc agcatataa
//
LOCUS       DAZGHF010000191         7044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGHF010000191 DAZGHF010000000
VERSION     DAZGHF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.192"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1114
                     /locus_tag="ACHK3A_004539"
     CDS             <1..1114
                     /locus_tag="ACHK3A_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO8742528.1"
                     /translation="IYFIKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCVLIFL
                     ALVNKVSENTKQRNLFSKKVTLCITLFFVLGGPIVAHIFSSHYESYDLHIAELTNKNG
                     QVVWKASYVTIMIFMWLTLLSVNLYFNGLRCDIWNGVSVIAFCAVLYNISLLFMSRYS
                     VSIWYISRTIEVVSKLTVLVIFMCHIFSALRVTKDIAHRDPLTNIFNRNYFFNELTVQ
                     SASAKKKTYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGE
                     EFGVLLTEIDTECAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQKNELNPK
                     EIYQLADNALYEAKETGRNKVVVKEAEILINRKDDE"
     gene            complement(1217..1304)
                     /locus_tag="ACHK3A_004540"
     tRNA            complement(1217..1304)
                     /locus_tag="ACHK3A_004540"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1268..1270),aa:Ser,seq:gga)
     gene            1537..2475
                     /gene="ghrA"
                     /locus_tag="ACHK3A_004541"
     CDS             1537..2475
                     /gene="ghrA"
                     /locus_tag="ACHK3A_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO8742529.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            2530..3267
                     /gene="ycdX"
                     /locus_tag="ACHK3A_004542"
     CDS             2530..3267
                     /gene="ycdX"
                     /locus_tag="ACHK3A_004542"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO8742530.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3291..3845
                     /gene="ycdY"
                     /locus_tag="ACHK3A_004543"
     CDS             3291..3845
                     /gene="ycdY"
                     /locus_tag="ACHK3A_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO8742531.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3947..4438
                     /gene="ycdZ"
                     /locus_tag="ACHK3A_004544"
     CDS             3947..4438
                     /gene="ycdZ"
                     /locus_tag="ACHK3A_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO8742532.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYAITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(4502..5335)
                     /gene="csgG"
                     /locus_tag="ACHK3A_004545"
     CDS             complement(4502..5335)
                     /gene="csgG"
                     /locus_tag="ACHK3A_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO8742533.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5362..5778)
                     /gene="csgF"
                     /locus_tag="ACHK3A_004546"
     CDS             complement(5362..5778)
                     /gene="csgF"
                     /locus_tag="ACHK3A_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO8742534.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5803..6192)
                     /gene="csgE"
                     /locus_tag="ACHK3A_004547"
     CDS             complement(5803..6192)
                     /gene="csgE"
                     /locus_tag="ACHK3A_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO8742535.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6197..6847)
                     /gene="csgD"
                     /locus_tag="ACHK3A_004548"
     CDS             complement(6197..6847)
                     /gene="csgD"
                     /locus_tag="ACHK3A_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO8742536.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1832 a         1557 c         1704 g         1951 t
ORIGIN      
        1 gatttacttt attaaaaccg tcattatcat tcagcaaatt attgaggggc gtttgacaag
       61 cagtgttgtt caaaatgaca ttgcaattta ttatttgttt cgtcagatga gtttgtgcgt
      121 attaatattt ctggcattgg taaataaagt tagtgaaaac acaaaacagc gcaatttgtt
      181 ttccaaaaaa gtgactttgt gcataacttt gttttttgtt cttggggggc cgatagttgc
      241 tcacatattt tctagccatt atgagagcta cgatttacac attgccgagt taaccaataa
      301 aaatggtcag gtggtttgga aagcctcata tgttaccata atgattttca tgtggttaac
      361 cttgctttcg gttaatctat actttaatgg tttacgctgt gatatctgga acggggtgtc
      421 ggtgattgct ttttgtgccg tgctgtacaa tatctctctg ttatttatga gcaggtatag
      481 cgtgtccatt tggtatatta gtcgcactat tgaagtagtc agtaagttaa ccgttttggt
      541 aatttttatg tgccatatat tcagtgcgct acgagtaaca aaggacattg cacatcgcga
      601 tcccttaacc aatatattta acagaaatta tttttttaat gaactgacgg ttcaatcagc
      661 atcggccaaa aaaaaaactt attgcgtcat gattatggat attgaccact tcaaaaaagt
      721 caacgacacc tggggacatc cggttggcga tcaagtgata aagacagtgg tgaatatcat
      781 tgggaaaagc atacgaccag atgacctttt agcgcgcgtt ggcggcgaag agtttggcgt
      841 cttgctgacg gagatcgata ctgaatgtgc gaaagcttta gcggaaagga ttcgggaaaa
      901 tgttgaacgt ttaactggcg ataatcctga atacgccata ccacaaaaag tgacaattag
      961 tattggcgct gttgtaacgc agaaaaatga gttaaaccct aaggagatct atcaactggc
     1021 tgataacgca ctttacgagg cgaaagagac cgggcgtaat aaggtggtag taaaagaggc
     1081 agaaattctt atcaaccgca aggatgatga atgagtctgt cgctagttgt cgacctccgc
     1141 ctggggcttc tcatccccca gtgtgtgaaa tatacgaaaa aaagctcgca ctttcgtacg
     1201 agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata
     1261 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg tcgttcctgt
     1321 cttgctggaa cgggcgctaa tttagggaaa tcatgacctg aggtcaacaa acttttttaa
     1381 aaaatcgctc gattattcaa acttcaatca atatgcagtt ttaataagcg aaatttgctt
     1441 tttttgccac cgaccacgga tttgttatgc tgctggtctt tgtagatcat aacgataagt
     1501 gcgaataaat ttcgccaaca ctcttcggga gtcagtatgg atatcatctt ttatcaccca
     1561 acgttcgata cccaatggtg gattgaggca ctgcacaaag ctattcctca ggcaagagtc
     1621 agagcatgga aaagcggaga caatgaatca gccgattatg ctttagtctg gcatcctcct
     1681 gttgaaatgc tggctgggcg cgatcttaaa gcggtgtttg cacttggggc aggtgttgat
     1741 tctattttga gcaagctaca ggctcaccct gaaatgctga agccttctgt tccacttttt
     1801 cgcctggaag ataccggtat gggcgagcaa atgcaggaat atgctgtcag tcaggtgctg
     1861 cattggtttc gacgttttga cgattaccgc atccagcaaa atagttcgca ttggcaaccg
     1921 ctgcctgaat atcatcggga agattttacc atcggcattt tgggtgcagg tgtactgggc
     1981 agtaaagttg cacagagtct gcaaacctgg cgcttcccgc tgcgttgctg gagtcgaacc
     2041 cgtaaatcgt ggcctggcgt gcaaagcttt gccggacggg aagaactctc tgcatttctg
     2101 agccaatgtc gggtattgat taatttgtta ccgaatacac ctgaaaccgt cggcattatt
     2161 aatcaacaat tactcgaaaa attaccggat ggcgcgtatc tcctcaacct ggcgcgtggt
     2221 gttcatgtcg tggaagatga cctgctcgcg gcgctggata gcggcaaagt taaaggcgca
     2281 atgctggatg tttttaatcg tgaaccctta ccgccggaaa gtccgctctg gcaacatcca
     2341 cgcgtgacga taacaccaca tgtcgccgcg attacccgtc ccgctgaagc tgtggattac
     2401 atttcgcgca ccattgccca gctcgaaaaa ggggagaggg tctgcgggca agtcgaccgc
     2461 gcacgcggct actaataaag catcaggttt cctgctatcc ttggcgggaa ttgaatacag
     2521 gagagagtta tgtatcccgt cgaccttcat atgcataccg ttgccagcac gcatgcatat
     2581 agcacattga gtgattacat tgcccaggcc aaacaaaagg gcattaaact ttttgcgatc
     2641 accgatcatg gcccggatat ggaagatgcg ccgcatcact ggcacttcat taacatgcgt
     2701 atctggccgc gagtggttga tggggttggg atcctgcgcg gcatcgaagc taacattaaa
     2761 aatgttgacg gtgaaattga ctgcagtggc aaaatgtttg attcgctgga tctgattatt
     2821 gccggttttc atgagccggt gtttgcgccg catgacaaag cgaccaatac gcaagcgatg
     2881 atcgccacta tcgccagcgg caatgtgcat ataataagcc atcccggaaa tccgaaatat
     2941 gaaatagatg ttaaagccgt tgctgaagca gccgcgaaac atcaggtggc gctggaaatc
     3001 aataattcct catttttaca ctcacgtaag ggcagtgaag acaactgtcg ggcggtagcc
     3061 gcagcggtac gtgacgctgg aggttgggtg gcattaggct cggattctca cactgcgttt
     3121 accatggggg aatttgaaga gtgtcttaaa atcctcgacg cggtagattt tccgccagag
     3181 cgcattttga atgtttctcc gcgccgctta cttaacttcc ttgaatctcg cggtatggca
     3241 ccgattgcgg aatttgcaga cctttaatta ctagtaatgg aaatatatta atgaatgagt
     3301 tttctatcct ctgtcgtgtg ctgggttcgc tctattaccg ccaaccgcaa gatcctttac
     3361 tggtgccgtt gtttaccctg attcgtgagg ggaaactggc tgcgaactgg ccactggagc
     3421 aggatgagtt actgacgcgt ttacagaaaa gctgtgatat gacgcaagtc tctgccgatt
     3481 acaatgcgtt gtttatcggc gatgaatgtg ctgtgccgcc atatcgtagc gcatgggttg
     3541 agggcgcaac ggaagcggaa gtgcgcgctt ttctttccga acgagggatg ccactagcgg
     3601 atacgccagc cgatcacatc ggcacattgc tgcttgcggc ttcctggctg gaagatcagt
     3661 caacggaaga tgagagcgaa gcactggaaa cactgttcag tgagtatctg ttaccctggt
     3721 gtggtgcgtt ccttggcaaa gtggaggccc atgcaactac gcctttctgg cgcaccatgg
     3781 caccgctaac ccgcgatgct attagtgcaa tgtgggacga gctggaagaa gattctgaag
     3841 agtaaatgtg atctgcatca ctcataactg caacgggtaa tttgttgttg cataaaatgt
     3901 gtgctcgatc tcattcatgg ccgcgttttc tgctatcatg cgcggcatga acatacttct
     3961 ctctattgca atcacaacag gcattctctc cggtatctgg ggatgggtgg ctgtttccct
     4021 gggcttacta agctgggcgg gcttcctggg ctgtacggcc tactttgcct gcccacaagg
     4081 tgggctgaaa gggctggcga tctccgctgc aaccctgctt agtggcgtgg tgtgggcgat
     4141 ggtcattatt tacggtagtg cactggcacc acatctggaa atactcggtt atgccataac
     4201 cggtattgtc gcgtttctga tgtgtatcca ggccaaacag ctgctgcttt catttgttcc
     4261 ggggacgttt ataggcgcat gcgcgacgtt tgccgggcag ggtgactgga aactggtgtt
     4321 accttcgctg gcgcttggac tggtatttgg ttacgcaatg aaaaacagcg gcctgtggtt
     4381 ggcggcgcgt agtgcaaaga cggctcaccg tgagcagcaa atcaaaaata aagcgtgagg
     4441 ggcactcacg ctttcgctta aaccgtaaaa tgtcggatga taattccggc atttttatct
     4501 atcaggattc cggtggaacc gacatatggc ggtatttcac tagaatgtca ttctgccgtt
     4561 ctgctttatt ttgcaaatcc caaagaccgc ggtcgatacc atcattaatc aggaaaatga
     4621 cgcctgtttc gatagccgac atcaggcaca gcataacggg ttcgttcgag gtgtaaccta
     4681 cttccccttc cagcaagcgc tggtagtcaa taaagcggaa gacccccgcc tgaacttcat
     4741 aggaaagtat cgtcttactg gtgttcaccg aggagaggat ctcgccggta ctcacattga
     4801 cgacgcgcag gttcacggca atctgatcaa gctggtattg cgtgtctgca ccgatcccaa
     4861 aatatcttgc cccaaccccg cccgatttaa cgttgctttc ataaccgata attgaacctt
     4921 caaccatgat atttgccgcc gttaaagatt gcagcgggat tcggttatta atggcaaccg
     4981 tgccgttttc ttgtgccgca cgaataatct tgcgctcgtt aagcaggttt tgtaagcctt
     5041 ggcgctccag cggtataaac cagcgagaat ctttcaatgc cgtgaccagc attgccgtgg
     5101 cgctttgcgg aacagcagtg gagaagttac ttgccgggta gggtttaaat tgcccggttt
     5161 cgtcctgaat gttgtatacc gaaacaaaga tcttacccgt cggcgctggc agatgggtca
     5221 aatctttgta gctctgagca cgaggcatta atgtcggtct ggcggcttct ttaggcgggg
     5281 cggttaagca tccgctcagt aacatgacgg caaccaaaag aaataagcgc tgcatgatta
     5341 ttttccttat gaagctgggg cttaaaaatc ggttgagtta ttttgtaaac ccgaaacctg
     5401 gatggtcgag gtttgtccgg ttttacgatc tgtcacgttc aactgcaatt gaccatcgcg
     5461 gttagcaata tcaacaatat aatcgttggt caccatgcgg cccggtttac cggtattaat
     5521 attcgacagt agcccaccca aaatttgtga ctggatggcc tgagtaaagt tatctaacgc
     5581 cgagggcgtt tcaataccaa agtcatcgtt atagctcgga tctttataag agttttgggc
     5641 ctgagcgcta tttaacaaaa aagcgccatt atttgggtta ccaccaaagt ttggattacg
     5701 gaactggaaa gtcatggttc cagcccaact taatggcgaa ataagcatga gtagaactac
     5761 tgcatgtttg acacgcattg cagcctccga acaatttatt atttaaaatt catcatgcgc
     5821 caaatcgccc gtacttaata acgcttggtt tatctggcga cgatttagtg cttcttcagt
     5881 ttgaatcagt gcaaagacga cagttttctc gaagtctctt ttcaacggaa ataaaaaagt
     5941 ctggaaaata acgtcctgat tcaccgttat tgtgatccag cttccccatc gtgcactggg
     6001 cctttcatta atcgttaagt tacccgtata gtcactttcc catttatcac taaaggctcg
     6061 gtaaaaatca tggccaatag atgaaacagt atggtcagtt aacaatcccg ggacttctac
     6121 ctcaacggcg tgaagattcc ctgcggcgaa cagaaattct gccgccacaa tccagcgtaa
     6181 ataacgtttc atggctttat cgcctgaggt tatcgtttgc ccaggaaacc gcttgtgtcc
     6241 ggttttttac ggctatcttc ttgaaaagat tataaagatg cgttttaacc gtattttcgc
     6301 tgatgaacaa cgaacgagcg atctcgttat tagacgcgcc gatacgcagc ttattcagga
     6361 tctctttttc ccgatgagta aggagggctg attccgtgct gttataacga tagttacctg
     6421 aatgcgtaat caggtaactg gcaagctttt gcgtaaagta gcattcgccg cgcaggacgc
     6481 cttgcaaccc attgacaaca cgttcttgat cctccatggc ataaaaaacg ccgttgatat
     6541 gaggccagtt ttcaatgtcg cggtacgggt aatcttcagg cgtatttagc aacaatattt
     6601 tgatattgtt gtttttcctg ctcaaagtat cctgccaata atggataagc tttttatccg
     6661 cttccatcat atccagaaga ataatagagc ctgaagagat atcgtccaga gaacgttgaa
     6721 tattatgtaa ttttcctgta attgccagcg attgcttaag gtgctgcaag agagctgtcg
     6781 cctgcaaaga aggtttagtg atcaacaata atgtatgacc atgaatacta tggacttcat
     6841 taaacatgat gaaaccccgc tttttttatt gatcgcacac ctgacagctg cctctaaaat
     6901 agaagcacca gaagtactga cagatgttgc actgctgtgt gtagtaataa atcagcccta
     6961 aatgggtaaa atataaaact aatggattac atctgatttc aatctagcca ttacaaatct
     7021 taaatcaagt gttaaacatg taac
//
LOCUS       DAZGHF010000192         6907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGHF010000192 DAZGHF010000000
VERSION     DAZGHF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.193"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(388..1278)
                     /locus_tag="ACHK3A_004549"
     CDS             complement(388..1278)
                     /locus_tag="ACHK3A_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO8742537.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFIH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
     gene            complement(1279..4251)
                     /gene="nfrA"
                     /locus_tag="ACHK3A_004550"
     CDS             complement(1279..4251)
                     /gene="nfrA"
                     /locus_tag="ACHK3A_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO8742538.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHGMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRH
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPRKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGIEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(4238..6475)
                     /gene="nrfB"
                     /locus_tag="ACHK3A_004551"
     CDS             complement(4238..6475)
                     /gene="nrfB"
                     /locus_tag="ACHK3A_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO8742539.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNPEQVAQLES
                     ENEGE"
     gene            complement(6673..>6907)
                     /locus_tag="ACHK3A_004552"
     CDS             complement(6673..>6907)
                     /locus_tag="ACHK3A_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8742540.1"
                     /translation="HWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGLRHIAINILT
                     NDKVFKAGLRRKLRKAAMDRNYLASVLAGSGLS"
BASE COUNT         1622 a         1806 c         1796 g         1683 t
ORIGIN      
        1 ttgctgtgtt ttttagtata aaaaagagga acaaaactga gacacataag gcctcgcaat
       61 ggcttgcaag gttttacata ttttgaggtg gtggaacgtg tgaacgcaga gatggcgcgg
      121 taatttgttg atttaaaatg tcgttcctgg agtttctaag tcgtgggcta caggttcgaa
      181 tcctgcaggg cgagccattt cctcgccatt tatttgttcc cttttacaaa ttacaccatc
      241 actttgttgt cgcggttttg ctaaataatt ggaacacgtc agacgaataa aatgaaaaca
      301 gaaattctgc taaacaattc atcttgccag gattttgatt aggaaagtga aaaggaaagg
      361 agcaagctaa gttgctccct ttaatcatta atactcaagg atactctgcg cgacgggcaa
      421 atagcgcaga gagaaataga gcgtgtcttt accgcaagag gaacgtttcg ctaacagcga
      481 ggcgatctct gcatccggtt tcggtttcgc tgtaaacgtt ttacctttac cagcgacaaa
      541 cagttcataa acaatgccgc ttgctggggc ggaactttgg caatcggcgc tggcctgtag
      601 ataacgttca cgctgttcag cggtcagttt atccacgccg ctgccatcct gtacccagac
      661 attaacaccg gttgctttaa cttgttccag cagttggcga tagccatctg gcgacatgtt
      721 tccggcgaaa aaactactga tataaaccgg ttttgctgaa acatcgctaa tcaaccgctg
      781 cgcgttgttt aaccatgtta gcaaaggttg acgggcggct tcgctgcgcc agttcaggtc
      841 gtcaatttcc gcgctgatgt accagccatc cggcgttacg ccaggcacgg cgctccataa
      901 tctggcttgc tggagatcgg cagccagcag gcgattaaga tagctttcca gcgctgcgga
      961 ggactgtttc tggtgtataa aaaattccgg atcggcgttc agcccgacaa tcagcttcag
     1021 gccagcctgt tgcgcagctg cggcacgctt aaacaataac gcgcgctgtt ctggctgggt
     1081 gaatgcatcg ccgtaacggg tccattgcaa aaccagggta tcgaagcctt gcaaacgtaa
     1141 ctgactcatc agcccctgcc actgggtatc ggtaacctga ctatcacggt tttgtggttg
     1201 ccagataata cctttcatcg caaaggaaaa agggctgacc aaaagcagtg tcaacaatac
     1261 gaaaatgaac ttacgcattt accagtgcac tccaatggtg agaaacgcgt tgttgcgctc
     1321 tccgttacgt tgattaatcg ccttaaaggt atgttgatat tcgatgccga gactgacttt
     1381 gtgcggccag gcgtcgtagt gcgtctcgcc ggtccagata ttccagcgga ccccgacacc
     1441 gccaagctgc gcgccctgag tgcctttatc acgataaccg ttgtcctgaa cgtgagcata
     1501 aggctcaata gtctgcccgt ttgccacctt ctgatgccag ctgactcgat aatctgccgt
     1561 ccacgcctga atatcctggc ggatatattg cgccgcatcg aggtacaggt tttgggcaaa
     1621 ccagcctgaa ccgttcgggt gccattcgtc gctgtatttg ccgccattaa agaatgaggc
     1681 gctggcgcgc agcatggtat cggatgcgcc attttgtccg ttcagcggca actgctgttc
     1741 gacggcgagg aaaaagatct gatcgcgcag cggcttccag cgcagaccgg tgccggacat
     1801 cggatttttc accggcatca tcaccccgtt ttcaccggta tcggcaaaga cgcggctata
     1861 aactgagagc aggtcgcctt ccagcagcat attgcgtccg atgcggtact cggcttccag
     1921 ttgcccgtag ctacgatagc ttttccttgg cgctgcgccg ccgacattat tgttagcggt
     1981 actcattgcg ccggaacgca agccaataga agaatcgaaa ctgaacgtcc agtggcgacc
     2041 gacctcctca tgcagacggc ggaaattgaa gcgttgctga ttttgctctg gggtcagtgg
     2101 ggttatcagc gcctgattat caatgtcatc aatcaccagt cgggcgtagt gctgcgtcgc
     2161 aggcatgtca tccagacgct ggttcacgta ggccagttgt cggataagtg ccggatcgtc
     2221 cggtagccct ttatgcgcct gttcgagcat ttcccgcgac tgtgcgatat caccgctatc
     2281 ccacaaggcg taaccgagcg ctgcctgggt gttgctatta ttcggttcca gttccagagc
     2341 ggcgcgcaaa tcactcaccg ccgccgggac attatgacgt tggcgataaa ttgtcgcccg
     2401 cgcaacgtaa gcgttggcag aaggcgcaat attgattgag cgcgtgagat cgctcagtgc
     2461 gagttccggc tgaccaggaa tgtaacgttg cgcatgcagc caccagtaga gggcattgtt
     2521 tcccagccca cgttgttctg cctgctgaag ccagcgatcg cgagccgcac catttcctgc
     2581 cgcctgggcg gtattggcag cagcaagcag atcctcattg ctcatgccgt gaagactgat
     2641 tttctgccag gccgccagtg cggtggcgta gtcctcaacc tgatacgcct gataggctac
     2701 cgcacgatgt tgccaggcgt tcggttgtcg ttgttcggcc tgaagccatg catacaacgc
     2761 cacaccgggt agcgtgtccc gataacactt tgccagacgg ttccaggcgg cagcatcgta
     2821 ggaaggcgac atatcgccca gcaagcgaac tattgccggg caattatctg caatacccgg
     2881 caactgactt tgccactgac gttgctccgc cagcggtaag ggtttcgata aaatcgccac
     2941 cttcgccgga gttgccaggt aaggatgact ttccagcaga gacgccagtc gcgccattaa
     3001 agtctggctg acacgcgcat cgccctggaa aggatagcgt tgcagcaata aatcggcagc
     3061 ttcacgtgac tgctcgttct gcatcagttg ccaggtcagt tgatccaggc gggtaagatt
     3121 tgccggttct tgctgataca gcaatcgtgc cagacgcaga gcttcagcct tgttacgggt
     3181 cgccacgcta acggtataac gctcctcaag catttcattg gcggggaggg tggcgagcag
     3241 tttttgcgct gcgtcgtact gaccttcttt taacagcacc ggtagcgtcg cgccaacaac
     3301 atactggcgg ttgtcggcaa actgtaccgt ataattcgcc aacgcctgaa cggggttgcc
     3361 gctgtattta gataacagat agagccaact tttctcttgt gcgtccgtgg taaatagcgg
     3421 cgtattttca atgagataat gctggaggcg tgctttttcg ccacgataag ccagcgcggt
     3481 cgcgtaagta atatatgact gaggatcggt gaagatcccc tgtgattgca gcgccaggat
     3541 ccgatcgtcc agctgcccgg caagaagcac atcaaaccac tgacggcgtt ctgccgcgct
     3601 taatgtgttc tgctggcgtg cttcattgta tagcgtatct gcctgggacc attgtttcag
     3661 gtagattgcc cgttgcagca gatcggttcg cagcgttttt ccttccggcg atgcagcaaa
     3721 cgtcgcatcg ttcagttgcg ctctggcgac aggtaattgt gccagccgca gggcattctg
     3781 cccgacttca ctgcgacaac gcagggtcgg cgcagcatcg cacgcttttt gctgggcaag
     3841 cagttcttca acagtcgtaa cgcttttcac ttcaactgga atagccgcca gactgcgctc
     3901 aagtcgggca tctcctgggt gacgtttcag ttgatcctca agcaacagcc gcgcccggtc
     3961 atcatgacca aaatggcgat aggcttccgc aaggtataaa gtcagcggaa tattatccgg
     4021 cacctgttgg tgtatatatt caaattcgcg gatggcggtt gcttcgtcgt tatttttctg
     4081 tgccttcagc gccttatcga gacggggata aataacaaaa tggcgataat cgctcagccc
     4141 cagctcttct gcgctggtgc cgatattgtc tgcgagtgcg ctggtgctca ataaagacgt
     4201 cagcagtaaa ccagaccatc cgatgacgcg attaaggtta ttctccttca ttttcggact
     4261 ccagttgcgc aacctgttct gggtttaaac ctgctttgag taatagtgat tgcatcgaaa
     4321 cttgtaattc gcgttgaatt gtcaggacgc gatccaacgt ttcctggctg ataacgcctt
     4381 cggtgaccaa aaacttgccg agcggcagag aactgcgttc atggcgcaat aacaacacgt
     4441 taattgctga acgattaata tgaccgagcg tggtcagtat ttcggcgaac aggaactgat
     4501 gcggcacata ttgccgccag atttcaccgg cctgctgttc cgtgagccac tgatgctgaa
     4561 ccgcattgta caacattgcc cgcgggtcgt gaccgcgtcg gcgtgcatac cagtgacgta
     4621 accccgtgac aatttgtccg cgcagaacaa tgacgtaacg cactttgcgt ccgactttac
     4681 gcgtcagggc tgccagcgaa accgggtcaa taccatcttc actgccgaca attaactcat
     4741 cattttccag acgcagcggc agcactgcat aatgcagcgc cacggaagcc ggcatttcgg
     4801 caatcagtga tgaagggatc tgccaggcgt cgatcgactc ccacgccacg ccgttttgct
     4861 ctgccagcgc ctgtgccagc tgttcggcgc taatcagccc ctgcatcagc attgaaccgc
     4921 ccaggcgtag accttcgacg cgattacgca gtgctgtatc gagttgttct tcagtgatga
     4981 cctgattttc cagcaggatt tggcctaacg ggcgcaacga gcgggtatcg ccagtcacgc
     5041 tggggaagtc atgcgttgtt ttatcccacg cgacgcgacg tggatcgccg tgttgaagta
     5101 cctgttttaa cgcgcgccag ttggccatga agttaatcag gttgccccag aaaagacgca
     5161 ggacagatag cagcccctgc gtcagaccgt agtagccagt gacaaaaatc acccgctgca
     5221 cgatgcggtt aaccatcaaa ccaaagttta gccacagcag ggtcattaac catgcgctgc
     5281 cgctgaaaat agaaaggaaa tgccaggcat cgggccacaa actttcatac gccagtaaca
     5341 gcaaaagctg gatcatcacc agcatcgcga ggaagctgac aaagttactg attgcccctt
     5401 tgcggtcgcg ccagagaaag tagttcagcg tcaggctgga ggtccatttg tgggttttaa
     5461 agccctggaa aacaatgccg atgatccagc gagatttttg tcgaaccgca gtcgaaaagg
     5521 tatcggggaa atattcgcgc acgcagatca tgtttgacgt ccgcgcgtgc tgtaaaaatt
     5581 tacgctgctc gcgttctttg gcttcgtcca ccaccggaaa acggacaaaa atttccgtca
     5641 tacctttttc tttcaggcgg aaaccaatgt cgtaatcttc agtcagactc tgcacgtcga
     5701 aagcaatacc gtcaccgtca gctaacagtg cggtcacggc gcggcggctg aaacaggtgc
     5761 cgacgcctgc gctgggcacc tgtccggcga gggcttcacg caccggaaca tctttgccat
     5821 gcagttctga aaactcatca atgtaggtca tgctggtgaa gtgcgtccat tcgcgttcga
     5881 acggatacac tgggatctga atcagatctt tacgctcgac cagatagttg aacagacgca
     5941 attccatcgg tgaaatcaca tcttcggcgt catgcagaat aaaaccagca aaagcgaaat
     6001 tggcgctacg ttcaaattgg gtgatggcgt ccagcacgtt gttcagacag tcggctttgc
     6061 tggtggggcc aggacgcgcg cagactacct tatgcacgtt cgggaagcga gcgcacactt
     6121 cgtcaacatc acgctgagta tcggggtcgt tggggtaggt gccaacaaag atatgatagt
     6181 tttcgtagtc gagcgtggtc gccgccagct cggccatatt gccgatgacg cccgtttcat
     6241 tccacgccgg aaccataatc gctaacggtt tttcatctgg tttatacagt tcgcggtaac
     6301 tcattcgcgg gtagcggcga taaacactca acttgcgttt aatgcggcgt acccagtaga
     6361 cgacatcaat aaaaaaatcg tccagcccgc tgatgaacat gatgaccgct aacgttatcg
     6421 cgattacttt taagccgtag agccaggtag caaaaacatc aagaagccag tccacacaaa
     6481 aaccttacat taacgctggt tatgtttagg gtggcgtata ttaaggtttt ttatgaattg
     6541 tgacagcttt ttaccattaa taggtatgac tattgcggca cgttattttt acactggtta
     6601 taaaagttgc cgtttgcgta gaatgtagac aaaagcgtgg tgtcaaggag gggaggttaa
     6661 cgcagggaaa gattacgaaa gcccgctccc agcaaggact gacgcgagat agtttctgtc
     6721 catggctgct tttcgcaact tacgtcttaa ccctgccttg aataccttat cattcgtcaa
     6781 aatattaata gcgatgtgcc gtagccctga aaataattct gctgcatttc ctcttcttat
     6841 tttgcagtcg tcttcattca ttaccacgtc cagacgccag tgcagcttat tctccacgtg
     6901 ccagtgg
//
LOCUS       DAZGHF010000193         6817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGHF010000193 DAZGHF010000000
VERSION     DAZGHF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.194"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            2..676
                     /gene="traX"
                     /locus_tag="ACHK3A_004553"
     CDS             2..676
                     /gene="traX"
                     /locus_tag="ACHK3A_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014654006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HFO8742541.1"
                     /translation="MLVWSPGQRDIIKTVALVLMVLDHANRILHLEQTWMFLAGRGAF
                     PLFALVWGLNQSRHAHIRQSAINRLWGWAVIAQFAYYVAGFPWYEGNILFAFAVAAQM
                     LKWCEYHSLVWSVSALTLLVLWFPLSSTSYGAVGIILLMFCHRIYRTTHLSVRSMLAL
                     CLFPVVALVNLQDSVTAAVSGVLVTYMVVRMVSGIRWPAARFWPADFFPVFYVCHLAV
                     LGVMAL"
     gene            756..1127
                     /locus_tag="ACHK3A_004554"
     CDS             756..1127
                     /locus_tag="ACHK3A_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HFO8742542.1"
                     /translation="MMKKILLVAAITGMFVQGVWAKSDQQIRDEAKNNVITVSQVSGM
                     TEGTGVTLNGQIVKQLAAEEDHFELSDASGSIVIDVDDEVWRPLALKAGDKVQVVGEV
                     DISDEGNRKATVDVLTLERLR"
     gene            1163..1720
                     /locus_tag="ACHK3A_004555"
     CDS             1163..1720
                     /locus_tag="ACHK3A_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO8742543.1"
                     /translation="MTEQKRPVLTLKRRTDVETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            1872..2075
                     /locus_tag="ACHK3A_004556"
     CDS             1872..2075
                     /locus_tag="ACHK3A_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742544.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            2763..4757
                     /gene="cirA"
                     /locus_tag="ACHK3A_004557"
     CDS             2763..4757
                     /gene="cirA"
                     /locus_tag="ACHK3A_004557"
                     /inference="COORDINATES: protein motif:HMM:NF007478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO8742545.1"
                     /translation="MFRFNPFIRHLLSTSIGASLFSFPVFTSAHSTLPDAAEDTLVVT
                     ASAVEQNLKDAPASISVISQDDLSRKPVHNLVDVLRDVPGVQITTDDNRKLVNIRGLG
                     GGYTLILVDGKRVSSRTAMFRNNDFDLSWIPVSTIERVEVVRGPMSSLYGADAMGGVI
                     NIITKKTGKEWSGVISGDMTLQEHRDRGDTYGGQFSAGGPLVENLLGIKVYGALSGRD
                     KDTGKQKSADGQETTRAEGFTTRNGGVDFTLTPLENHEFSAGYSFDRQDRESEGSSDK
                     NRMERQNYSVGHNGQWGLGNSELRFYSEKVNNLNTGNTHPITSWNEVIDGKYVFPFTD
                     YNHIVILGGDYRREKISDPVHLTGEKGSENSTSQYSLFAEDEWRLFAPLALTTGVRMD
                     KHENYGTNWSPRAYLVWNATDSVTVKGGWARAFKAPTLIQQSEAWLTNSCKGGCQIIG
                     SPDLKPETSESIEGGVYYSGQEGLLEGVEASVTVYRNEIDDMIANKRSKKLKEAMTLP
                     NFVGITDDGRPVFRYFNINKARIQGLESELKIPLGEQVKLSLTYTYTDGRDLSSGEDK
                     PLNDSPFHNANGRIDWTPVDDWSFYVSANYHGETRAQSSGTETQGGYTLWDAGASWKA
                     TKNITVRSGILNVMDKDLERNDYSYTVDGRRYFLSMDYRF"
     gene            5180..5641
                     /locus_tag="ACHK3A_004558"
                     /pseudo
     CDS             5180..5641
                     /locus_tag="ACHK3A_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            6007..6390
                     /locus_tag="ACHK3A_004559"
     CDS             6007..6390
                     /locus_tag="ACHK3A_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742546.1"
                     /translation="MNYQAVSELITSSNHNVLIVGDSASEVDSFLNKLNITDYKYYDF
                     RQVYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGK
                     QLIVVVREQDMKKMEAEFGRIIKIH"
     gene            6670..>6817
                     /locus_tag="ACHK3A_004560"
     CDS             6670..>6817
                     /locus_tag="ACHK3A_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8742547.1"
                     /translation="MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
BASE COUNT         1949 a         1311 c         1801 g         1756 t
ORIGIN      
        1 gttgctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac tggtgctgat
       61 ggtgctggac catgcaaacc gcattctgca tctggagcag acgtggatgt ttctggccgg
      121 gaggggcgcg tttccgctgt ttgcgctggt ctggggactg aatcagtccc gtcatgcgca
      181 tatccggcag tcggccatta accggctgtg gggatgggct gtgattgccc agttcgcgta
      241 ttacgttgcc ggctttccct ggtatgaggg gaatatcctt tttgcctttg cggtggcagc
      301 acagatgctg aaatggtgtg aatatcactc gctggtatgg tcagtcagtg cacttacact
      361 tctggttctc tggttcccgt tatcttccac cagttacggt gctgtcggga tcatattact
      421 gatgttctgt cacaggatat acaggacgac gcacctttct gtacggagca tgctggcgct
      481 ctgtcttttc ccggtcgttg ctctcgttaa cctgcaggac agcgtgactg ccgctgtcag
      541 tggtgtgtta gtgacataca tggtcgtcag gatggtatcc ggtatccggt ggcctgctgc
      601 ccgtttctgg ccggcagact ttttcccggt attttatgtc tgtcatctcg ctgtgctggg
      661 agtgatggcc ctgtagcagg aagcgcaaga aagcggatga actgtgaaag tctttttgtt
      721 taaatcagaa cgcattcagg aaaaaacagg agcagatgat gaaaaaaata ttactggtgg
      781 cagccattac gggaatgttc gttcaggggg tatgggcaaa atcagatcag caaatccggg
      841 atgaggctaa aaataatgtg atcactgttt ctcaggtgag tggcatgacg gaggggaccg
      901 gtgtgacgct gaatggtcag atagtgaagc aactggcagc cgaagaggat cattttgaac
      961 tcagtgatgc atcaggaagt attgtcattg atgtcgatga tgaagtatgg cgcccgctgg
     1021 ccctgaaagc cggagataaa gttcaggttg tgggagaagt ggatatttct gatgagggaa
     1081 accggaaagc cacggtggat gtactgactc ttgagcgtct tcgctgatat gaacaacgga
     1141 taccggtgag ggcaggtgag ttatgacaga gcagaaacgg ccagtactga cactgaaacg
     1201 aagaaccgac gtggaaacac cgacccggag ccggaaaacc atcatcaatg tcaccacgcc
     1261 accaaaatgg aaggtgaaaa agcagaaact ggcggagaag gctgcccggg aagcagagct
     1321 ggcggcgaag aaagcacagg ccagacaggc gctgtccatt tatctgacgc tgccctcgct
     1381 ggatgaggcg gtgaacactc tgaaaccctg gtggccggga ttgtttgacg gtaacacgcc
     1441 caggcttctt gcctgtggta tccgggaggt tttactggat gaggtgtttc agcggaatat
     1501 cccgctctct cataaaaaac tcagccgggc gctgaaagcc atcacccgtt cggaaagtta
     1561 tctgtgcgca atgaaagccg gcgcctgtcg ctatgatacg gaaggctatg tgacagaaca
     1621 tatcacacag gaagaagaac agtatgcgca ggcgcgcctg gaaaaggtgc gcagacagaa
     1681 ccggatcaag gatgagctgc gggcgatcct ggcggaataa aagagccctc tgtatgacag
     1741 agggcaacag cgagaaaaaa tcgggaaaac agaaatccag agttttgatg gagaaaacac
     1801 ccaaccgttc agggatgttt cagaatgttt ctgacataat ccgggagccg ggtcaagagg
     1861 ggggataaaa aatggctgta tccagtggat tatctgagca cagcggtatt tatcgctatc
     1921 agcgcgtggg gcgtcatgcc gtccgtcagg agattcgtgg ttttttcagg gaagccgctg
     1981 aggcctggca gaatgcggcc cggctggcac cctgtgaagc atggcgggat tttgccctga
     2041 agagggcatc tgcatgccgg tgtaagtgcc gataaaaata acccccgtcg cggagctccg
     2101 ggggatcagg tggcagatcg ttattattat tatagtggtg taagaaggag ttgccctgtc
     2161 tttcaaacgg acatcacaga gttaacatat ttcacatccc gggtaagcca gcagggataa
     2221 aaaatgtgat gaatttcaag aagcaggagg tgacagtgga atgcataaca gcagggttat
     2281 ccggtgaatc tgacagacag ccgggcagta ttcatggcag gataaagaaa aaccgggaga
     2341 tgactctccc ggtccgtctt atcgctaatc ccctgagcag ggcatgtatg acttatctat
     2401 gaccgttata ttcagcgcca catccggtag cctgcgatat aacacagtta tcctatcagc
     2461 aagagcagga atgtttaata gtcaggggtg aaaaatataa tctgtatcac aaagtagtat
     2521 gtagcagggg gaagccgata gggtacgaac ggggaacggg aatacatgac cttgcgaaca
     2581 ggaaagtatg tatagtaaag tagagaagaa tggtattgac ataaaaatat caataaataa
     2641 attttaatca aagaagtata tcaatattct taatataaga tggggcaaac tccatcatat
     2701 ttatggaagt cagtgtgggt aaatcttgca acattgaata caaattatgg atataatcag
     2761 aaatgttcag atttaaccct tttattcgtc atcttctttc aaccagcata ggtgcatcgt
     2821 tattttcatt tcctgtgttc acttctgcac attcgacact tccggacgct gctgaagata
     2881 ctctggtagt tacagcatct gctgttgaac agaatctgaa agacgcacct gccagtatca
     2941 gtgttattag ccaggatgat ttatcccgta agccagtcca taatctggtg gatgttttaa
     3001 gggatgtgcc cggggtgcag attaccaccg atgataacag aaaactcgta aatataaggg
     3061 gactgggcgg aggatacacc ctcattcttg tggatggtaa acgggtcagt tcccgcacag
     3121 ctatgttccg gaataatgac tttgatctga gctggatccc tgtttccact attgaacgtg
     3181 tagaggtcgt gcgtggccct atgtcatcac tgtatggtgc ggatgcgatg gggggcgtca
     3241 ttaatatcat tactaaaaaa acaggtaagg aatggtcagg tgtcatctcg ggggatatga
     3301 cgttacagga acacagagac cgtggcgata cttatggggg acagttcagt gcaggtgggc
     3361 ctttggttga aaacctttta ggaattaaag tttacggagc tttaagtggc cgggataaag
     3421 ataccggaaa acaaaaatcg gcagatggac aggaaaccac acgtgctgaa ggttttacaa
     3481 cccgtaatgg tggcgttgac tttacgctta ctcctctgga gaatcacgag ttcagcgcag
     3541 ggtattcttt tgaccgtcag gaccgggagt ctgaaggtag tagtgataaa aaccggatgg
     3601 aacgacagaa ttattctgtt ggccataatg gacaatgggg attagggaac agtgaactcc
     3661 gattttacag tgaaaaagta aataatctga atacaggtaa tacacatccg ataacttcat
     3721 ggaatgaggt tattgatgga aaatatgtat tcccattcac tgattataat catatcgtga
     3781 tattaggtgg tgattatcgc agggagaaaa taagtgatcc tgtccacctt accggagaga
     3841 aaggttcaga aaattcgaca agtcagtatt cactttttgc agaagacgaa tggcgtttgt
     3901 tcgcgcccct ggctttgaca acaggggttc gtatggataa gcacgaaaat tatggaacca
     3961 actggagtcc aagagcatat cttgtatgga atgcaaccga ttctgttact gtaaaaggag
     4021 gatgggccag agcatttaaa gctccgaccc tgattcagca aagtgaagca tggctgacga
     4081 actcatgcaa agggggatgt cagatcatcg gtagccccga cctgaaaccg gaaacaagtg
     4141 aaagtattga gggtggtgtt tattacagcg gacaggaagg actcctggaa ggtgtggaag
     4201 caagtgtcac tgtgtatcgt aatgaaattg atgacatgat tgcgaacaaa cgcagtaaga
     4261 aactgaaaga ggcaatgacg ctgcctaact ttgtcgggat aacggatgat ggacggcctg
     4321 ttttcagata ctttaatata aataaagcta ggatacaggg gcttgaatca gaactgaaaa
     4381 tcccgctggg agagcaggtg aaactgtctc tcacctatac ctatacagat ggacgggatt
     4441 tgagtagcgg agaagataag ccactgaatg acagtccgtt tcataatgca aacggaagaa
     4501 ttgactggac tcctgtggat gactggtctt tttacgtatc agctaattac catggggaaa
     4561 cccgggcaca atcttccggc acagaaacgc agggggggta tacattatgg gatgccggag
     4621 cttcctggaa ggctacgaaa aatataactg tccgttcagg tatattaaat gttatggata
     4681 aagatttaga acggaatgat tacagttata ccgttgacgg gagaagatat tttctgtcaa
     4741 tggattatcg tttttaatag taaaccgaag gctccaagag gagcctttat aagataatgt
     4801 tctgaaaata aacaagacga taagaatgaa gtaagatgcg gatgaataaa gaaagtaaat
     4861 tatatttacg gaatcaggta aagttcattt ttataagaat tcattttctg cggtctttca
     4921 ttatgaagtt atcccatata tctgaaaggt aattaaggct actattaact aaaaaacata
     4981 cgttaacctt cgtacagaat gcatgattaa ttcatgagac cttagtaaca catggctata
     5041 tgtgtattca gccactgaat cttattttac tgaatagacc acaatttaac ggtttatttt
     5101 tggcgtgatg agtgaggcat tattcatcac ggaatggaat tcctgcaatc acaaaaagta
     5161 atcagtgacg gagagtaaaa tgaagaaata tataccagtg gtattgtttg ttttttcata
     5221 tcaggttttt tctgctgata ttcatggacg ggtcgtgcgt gttctggatg gcgataccat
     5281 tgacgtcatg ttatctcagc atccggtgcg tgtccgtctt gttaatattg atgcacctga
     5341 aaagaaacag gactatggac ggtggactga aaaaataatg taatctctgg ttgcgggaaa
     5401 aacggttact gtcacatatt ttcagcgaga tcgttatggt cgtattctcg ggcaggttta
     5461 tgcaccagac ggaatgaatg ttaatcagtt tatggtccgt gcaggcgctg catgggttta
     5521 tgagcagtac aacaccgatc ctgttcttcc cgtcctgcag gaagaagcac gacagcagaa
     5581 acgtggtctg tgggcagaca gcgcgcctgt tccgccctgg gtatggcgct cacggaaata
     5641 atgatatttc tgtggctatt gagttctcat tgtaatgcga tatttaaaag catgaagcat
     5701 atttcgttat taactggggt cagtttggat atagagaatt attgtacggt aagcctgttt
     5761 tttgaacagg cttacctata gcaatagggt gtactcccgc gctatacggt gttttcagga
     5821 gaattggtct taatcggata acctaaatgc gatacagcaa gagcgacaac ttaaaagcga
     5881 taactgctga cgtaaaatca atccgttaca catcgggatt ggttaaataa tgcgcttaac
     5941 gtacaaaaat ttccgatctc caaactgacc ccttaactta tctctctgtt aatgaaaggt
     6001 gaacgaatga actatcaggc agtatcagaa ttaataacat cgtctaatca taatgttctt
     6061 atagtggggg actcagcttc tgaagttgat agttttttaa ataaactgaa tatcactgat
     6121 tataagtatt atgatttcag gcaggtttac agttgttctg acagaacatt aaatgattat
     6181 gcggttattt ttatcaggga tgcactgaac gccagtgagc acattattat ttttaattgt
     6241 acaggatggc ctgatttgaa taatgaatct gccgtcatgc agtttgccag agtggcacga
     6301 aaatcaggaa aacaactgat agtcgtagtg agagaacagg atatgaaaaa aatggaagca
     6361 gagtttggaa ggatcataaa aatccactga cgccttcgcg ttgctcagtt gtccaacccc
     6421 ggagacggga aaaagcaagt tttccccgtc cccggcgttt ctgtagtgaa aagcgggaaa
     6481 tggagggaat ggtcagtaac agggaatgtg gtattatcgc ggcgggtgtc tgagcctttc
     6541 tggttcaggc aagacgcagg taccagaaat gcgaagaccc cacttgttaa tccattaact
     6601 cgtgaggcct gcatgaagta tctcaacact actgattgta gcctcttcct tgcagagagg
     6661 tcaaagttta tgacgaaata taccctaatt gggttgctcg ccgtgtgcgc cacggtgttg
     6721 tgtttttcac tgatattcag ggaacggtta tgtgagctga atattcacag gggaaataca
     6781 gtggtgcagg taactctggc ctacgaagca cggaagt
//
LOCUS       DAZGHF010000194         6799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGHF010000194 DAZGHF010000000
VERSION     DAZGHF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.195"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(112..780)
                     /gene="artM"
                     /locus_tag="ACHK3A_004561"
     CDS             complement(112..780)
                     /gene="artM"
                     /locus_tag="ACHK3A_004561"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO8742548.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(780..1496)
                     /gene="artQ"
                     /locus_tag="ACHK3A_004562"
     CDS             complement(780..1496)
                     /gene="artQ"
                     /locus_tag="ACHK3A_004562"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO8742549.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTVLRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1503..2234)
                     /gene="artI"
                     /locus_tag="ACHK3A_004563"
     CDS             complement(1503..2234)
                     /gene="artI"
                     /locus_tag="ACHK3A_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO8742550.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2252..2980)
                     /gene="artP"
                     /locus_tag="ACHK3A_004564"
     CDS             complement(2252..2980)
                     /gene="artP"
                     /locus_tag="ACHK3A_004564"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO8742551.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3198..3713)
                     /gene="ybjP"
                     /locus_tag="ACHK3A_004565"
     CDS             complement(3198..3713)
                     /gene="ybjP"
                     /locus_tag="ACHK3A_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8742552.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3839..4162
                     /gene="ybjQ"
                     /locus_tag="ACHK3A_004566"
     CDS             3839..4162
                     /gene="ybjQ"
                     /locus_tag="ACHK3A_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO8742553.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4159..4989
                     /gene="amiD"
                     /locus_tag="ACHK3A_004567"
     CDS             4159..4989
                     /gene="amiD"
                     /locus_tag="ACHK3A_004567"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO8742554.1"
                     /translation="MRRVFWLVAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTLVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4986..5999)
                     /gene="ybjS"
                     /locus_tag="ACHK3A_004568"
     CDS             complement(4986..5999)
                     /gene="ybjS"
                     /locus_tag="ACHK3A_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO8742555.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6098..>6799)
                     /locus_tag="ACHK3A_004569"
     CDS             complement(6098..>6799)
                     /locus_tag="ACHK3A_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO8742556.1"
                     /translation="VWFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAF
                     DDAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKASASLAALWQVV
                     NQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEK
                     QLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFR
                     GMAKRIARLAEQSTD"
BASE COUNT         1653 a         1788 c         1800 g         1558 t
ORIGIN      
        1 taaaaaaaga cggacaactt agtgagttgt ccgtcttcat tataagaatt tatgcactat
       61 gtaggccgga taaggcgtcc gcgccgcatc cggcatcagc acgcagccaa attaattcct
      121 tcgctcaaac gccagcgctt tacgctcgat cagacgcatc atcagcgtca gcaggccgtt
      181 aacgaccaga taaataatcc ctgccgcgcc gaacaccatg acatcgtagg tgcgtccgta
      241 caacaactgg ctgtatccca tcacttccat cagcgtaatg gtgtaggcca gcgaggtact
      301 tttgaacacc agcactactt cgttggaata agaagagagc gagcgtttaa aggcatacgg
      361 cagcaggatc gccagcgtat cttttttgct cattcccagg gcgctacagg actgccactg
      421 accttccggg atcgcacgaa ttgcaccgta aaacagctgc gtggtatacg ccgcactgtt
      481 cagcgacaac gcaatcagcg cacacagcca cggttctgac aacaaatgcc acagtgccgg
      541 atactcctgc aacgtcggga actggcccgg tccgtaataa atcaggaaga tctgcaccag
      601 cagcggcgta ccggtaaaca gcgtgatata gccccgcacc agccacacca gcaccggcgt
      661 tttcagcgtc aggatgatgg taaaaatcaa tgccagaatc agtgccacaa tcagcgaggc
      721 aacggttagc gtcaggctgg tgtgcaaccc tttcatcagt tcgggtaaat actcaaacat
      781 tagctgggcc tccgctcaaa acgtgtcgcg cgcaggtcaa tgcgtttgag aatgtactga
      841 ctgagcaggg tgatcaccag gtaaatcgcc gccgccacaa tgtaccaggt aaacggttcc
      901 tgggtacggg tagcgatgct tttggtttgt agcattaaat cattcacact aatcaaactg
      961 accagcgcgg tatctttcag cagcaccagc cactggttac cgagacccgg cagcgcatga
     1021 cgccacatct gcggcatcac cagacggaaa aagatagccg atttcgacag ccccagcgcc
     1081 tgaccagatt cccactggcc caccggcacc gctttcaacg cgccgcgcag cgtttgcgag
     1141 gcataggcgg catacagcag tgacagagcg atgacaccac aaaggaacgg gctaacgtcg
     1201 aagttctcaa tgtccatctg caccgggatc tgcacgaacc caagattgat agtgaagcca
     1261 tccgaaagcg tcagcagcag ctgcgaggag ccaaaataga taaacagcac caccagaatt
     1321 tctggcaggc cgcgcagaac cgtcaccaac gctgaacctg cccacgcgac aggacgccat
     1381 tttgcagact cccataccgc aaagaacatc gccagcgcca gcccgacaat caatgcacaa
     1441 acggcaaggc cgacggtcat cccggcggcg cttgctaaag gaaaaaattc attcatcagg
     1501 aattacttct ggaaccattt gttgtagatg gtttcgtaag tgccatcttt cttcactttt
     1561 tccagcgcag tgttgagttt ctgctgcagt tcagtgttgc cctggcgtac tgcgatgccg
     1621 aggccagtac cgaagtaatc tttatcggtc actttatcgc ccactgccgc cagtttcggg
     1681 ttatctttca gccattcggt taccactgcg gtgtcaccaa atacgctgtc gatacgaccg
     1741 ttttgcagat ccagttttgc gttctggtaa ctgtcatatg gcacggtagt gatttccgga
     1801 tgcttatcca taatgaactt ctggtgtgtc gtcccgttct gaacgccgac ttttttgcct
     1861 ttcagctgat caacactggt gtatttgcct tgctgaccca caaacagggc agagttgtca
     1921 tagtacgggg tggtaaacag cacctgcttt tcacgctccg gagtgatatc catgcccgcc
     1981 atcactgctt ctacgcgacg gaatttcagg cttgggatca ggctgtcaaa cgcctggtta
     2041 gagaaagtgc aggttgcatc aatctcttta cacaatgcct gcgccaggtc gacatcaaaa
     2101 ccaacgatct ggttgtttgc atcaatcgat tcaaacggag gataggaggc ttcggtggca
     2161 aaacgaatgg tttcggctgc tgtggcggaa agactaaaac ctgcaattaa cgcggcaatc
     2221 agaacttttt tcattgttgt tatcccgaat cttagtgaga gagatagttt ttaaatgctt
     2281 cggtttgcgg ctcggtaaag cagctcgcgt cgccttgttc tacgatatga ccgttttcca
     2341 tatacaccac tcggctggcg gttttacgcg ccacttcaac ttcgtgggtg acgatcacct
     2401 gggtaatatt cgtttctgcc agctcacgaa tgatgctgac gatttgtgcc gtaatttcgg
     2461 ggtccagtgc ggcggtcggt tcatcgaaca gcagtacctg cggttccatc atcaacgcac
     2521 gggcaatagc aacacgctgc tgctgacctc cagaaagatg cagcgggtaa cgatcgctat
     2581 aaggtttgag acgcagacgt tccagcagtt tttctgcacg ggccagcgcc tgatctttac
     2641 tcaaccccag tacacgacag ggcgcttcaa tcaggttttg ctgcacggtc agatgcggcc
     2701 acaggttgta ttgctgaaac accatgccaa cgttacgacg caaatcgcga atcgctttgt
     2761 cagagggggt tttggtgaaa tcgaaatggt tgcctgcaat gttgagcgta ccggagcgcg
     2821 gcatctcaag cagattgagt acacgcagca gcgagctttt acccgcgccg ctggggccaa
     2881 gtaacaccag cgtttcgccc tgtgggcaat ccagcgtgat atcgaacagc gcctgatgcg
     2941 cgccgtagaa gcaattaatg ccgtttaatt gaatactcat tgacactcgt atactggcag
     3001 tctgatagct attgaggtcg aagatagtac ctttgacaga ataattatgc aatatttctg
     3061 ctttaaaagt taaaagcaaa gcgcattatt caataaacat agcacaaaat aacgggggcg
     3121 gtggtcggcg agcataaatg tcggcattcc tcacgaaatg ccgtacaatt tacggggttt
     3181 attggttgat caagacgtta gcgattctcg atggactgac ggagcgtacc cgctgtggca
     3241 tgaacgctac cgcctaagta gcgcacatcg tcgatgaccc aacactggcc ttcctgaatc
     3301 attaacactt catcctgcca gccctggtca ccctgcttga gatccacgcg caatggaatg
     3361 ttacgggcat cacgattagg gatagtcgat gcactggcaa cgtgggcgct gtctggcaag
     3421 gtggttcgac tggagaacgg atcgctgctt aacaattcac gatgactatt atcgcggctg
     3481 gcatcgctaa gtagggttgc cagtttgtca ctcaaatagg ggcgcagagc ggtaatgtca
     3541 ttgccgcgat gttgaatgcg gtagtcatag aactgttgag ccacgctatc aggccccccc
     3601 tggacacagg aaccggtgcg ggggccgtta tctttataag ctggcgtgac ggtggtacag
     3661 gcactgagta gcagtgcgca agggataagc atggtcaatt tgctatagcg catgatgatt
     3721 tccttataag cgaacacctt gaaagcgttc tacgataata atgatatcct ttcaataata
     3781 gcgtatcagt ccgataatgc tttgaagatc gaaggcttag caaacaagga gatcgatcat
     3841 gcaattttct acaaccccaa ctctggaagg ccagaccatc gtcgaatatt gcggtgtggt
     3901 gaccggcgaa gcgattttag gtgccaatat tttccgtgat ttctttgccg gtatccgcga
     3961 tatcgttggc ggacgctccg gggcttatga aaaagaattg cgtaaagcgc gggagatcgc
     4021 ctttgaggag ctcggcgatc aggcccgggc gctgggggct gatgccgtag tcggtattga
     4081 tatcgactac gaaacggtcg ggcaaaatgg cagtatgctg atggttagcg tcagcggtac
     4141 ggcggtgaaa acgcgtcgat gagaagagtc ttttggctgg tcgctgtcgc tctgttactg
     4201 gcagggtgtg caggcgaaaa aggcattgtc gagaaagagg gatatcagct tgatacccga
     4261 cgccaggcgc aggcggcgta tccgcgcatt aaagtgctgg ttatccacta caccgcagat
     4321 gattttgata gctcgctggc gacactgacc gataagcagg tcagctcgca ttatctggtc
     4381 cctgcggtac caccgcgata caacggtaaa ccgcgcatct ggcaactggt gccggaacag
     4441 gaactggcct ggcatgcggg gattagcgcc tggcgcgggg caacgcgtct taacgacacc
     4501 tctattggca ttgagctgga aaaccgtggc tggcaaaaat catccggagt gaaatatttt
     4561 gccccgtttg aacaggcaca gattcaggcg cttattccgc tggcgaaaga tattattgcc
     4621 cgttatcaca tcaagccgga aaacgtagtg gcgcatgcgg atattgcgcc gcagcgcaaa
     4681 gacgatccgg ggccgttatt cccctggcaa caactggcgc agcaggggat tggtgcctgg
     4741 ccggatgcgc agcgggtgaa cttttacctt gccgggcgcg cgccgcacac actggtagat
     4801 accgcatcat tactggagct tttggcgcgc tacggttatg acgttaaacc tgatatgaca
     4861 ccgcgcgagc agcggcgcgt gattatggcg ttccagatgc atttccgccc gacgttatac
     4921 aacggcgaag cggatgcaga aactcaggcg attgccgaag cgttgctgga gaaatacggg
     4981 caggattagc gcggcagttt tccgtggtcg cgtagccagg cggcagtttt ctcgatacct
     5041 tcatccaggg tgatgaccgg ctgataacct aactcttcct gcgcgcgcgt aatatccagc
     5101 gtaaagtcaa aattcaactt ggagacgccg tagtgggtca gcggcggctc ttttgctgac
     5161 ttgcggccta aacgctccat gctgcgggcg atcatatcca gcatcgggta ggggacggaa
     5221 cgaatacgac agtcaatatt caactcgtcg atcagcttct gcacgatgct gcgcagtgtg
     5281 cgatgctcgc cgttggtgat gttgtacaca cgcccggaag gtagcttatc gcaggcttcc
     5341 tggcttgcca gccacatggc gtgcacggca ttttcatagt aggtcatatc gaccagcgca
     5401 ctgccgccat gcggtaacag aatgctgccg tagtggtgca tcatatgcgc cagacgggga
     5461 ataaagactt tatcgtgcgg tccgaacaga ctttgtgggc gcagaatagt aaagcgcgtt
     5521 tgtggattcg cctgcgaaag catattgatc acctcttcgc tggctgcttt gctacgggca
     5581 aactcgttgg cgaagcggtg agggcgaaaa tcttctttaa tatcgcgatg gtggtgataa
     5641 tcgaagtaca gggagggtga agagatatga ataaagttac gcacacccca ggcgacagcc
     5701 cattcaccca ggcggcgagt ggcgcgaacg ttagccagat cgaaagcctg ttgtgtcccc
     5761 cagggcgagg taaagctgga gcagtgccac agcgtatcaa tgcccgcgag catcacttta
     5821 gcttgtgatg agaccagctc ggtcagatcc gccggaacaa actctgcgcc cattttttcc
     5881 agcaatttac ccattgcctc gttgcgaccg gtcgctcgca cgctgatgcc tttctggcat
     5941 aaaaactcta ccgcgtttcg acctaagccg ctggtggcgc cggtaaccag taccttcata
     6001 tcaatccact gttgttgaga aaataacgtg cgcattcttc cgtgatttcc cccatgatgc
     6061 aatgggaaac atgaaagaat aacgcaggtt ttgtcgatta atctgtgctt tgttctgcca
     6121 gtctggcgat tcgttttgcc attccgcgaa aaataaacag atgcgcgggg atcatcaata
     6181 accagtaaaa cagccccggc ataccgtgcg gatgccagaa tgcgcgaaca tcgatagtac
     6241 gatagtcacc tttatcttcc agggtaaaac acagtcgtcc cagccccggc gctttcatgc
     6301 caaataacag cgtaagttgt ttttccggtt caacgacaat cactttccag ctatccaccg
     6361 catcgccggt ctgcaaatat tcacgctccg ggcggccttt cgccagctta tgaccgatcg
     6421 cgcggtccat caacgcccgt gtctgccaca aaatattgcc aaagaaataa cgctctttac
     6481 cgccgatttg attcaccacc tgccataaag ccgcaaggct ggcggacgct ttaacggtaa
     6541 accccgcctg tttggcgaaa taaccgtact ccggtcgcca gcgggcaaag gcctgagcgt
     6601 cgtagcccca gtcgctggag ttgaccagct tttcttcttc tttcagggtg cgacgtaccg
     6661 cgtcatcgaa agcgatcagc cgttgtggga tgagtgcacg tagcgcggtg tcatccgcca
     6721 gcagatcatg tttcagcccc tgaatcaacg ccctggcggt ggtgggcggt acggaagtaa
     6781 tcacattgag aaaccacac
//
LOCUS       DAZGHF010000195         6795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGHF010000195 DAZGHF010000000
VERSION     DAZGHF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.196"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(102..812)
                     /locus_tag="ACHK3A_004570"
     CDS             complement(102..812)
                     /locus_tag="ACHK3A_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO8742557.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKVN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYK
                     GKDNKHENSYRVGVLINF"
     gene            complement(858..1688)
                     /locus_tag="ACHK3A_004571"
     CDS             complement(858..1688)
                     /locus_tag="ACHK3A_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8742558.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDNSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            complement(1692..3164)
                     /locus_tag="ACHK3A_004572"
     CDS             complement(1692..3164)
                     /locus_tag="ACHK3A_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8742559.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            complement(3213..4088)
                     /locus_tag="ACHK3A_004573"
     CDS             complement(3213..4088)
                     /locus_tag="ACHK3A_004573"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO8742560.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            complement(4121..5038)
                     /locus_tag="ACHK3A_004574"
     CDS             complement(4121..5038)
                     /locus_tag="ACHK3A_004574"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO8742561.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKKMKII"
     gene            5156..5708
                     /locus_tag="ACHK3A_004575"
                     /pseudo
     CDS             5156..5708
                     /locus_tag="ACHK3A_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(5942..6628)
                     /locus_tag="ACHK3A_004576"
     CDS             complement(5942..6628)
                     /locus_tag="ACHK3A_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO8742562.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSIASMMAQMQMGDVKALFENKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
BASE COUNT         2087 a         1669 c         1179 g         1860 t
ORIGIN      
        1 aatggttcag gaatttccgg taaagctccg gctaccgacg ttccaataaa caagcagaag
       61 ggaagatata taaagttttt cactttcatt gtaaccacct tttaaaaatt aatcaaaaca
      121 ccgacacggt aactattttc atgtttatta tctttccctt tatacacccc ttgtttatcc
      181 aggtaatcat attctatata tggggtaaac accggtgaca ttttatattg cagaacaaat
      241 gcattttcag ttgctgtttt tttatgattc ccatatttga aattattaac cttcgtatat
      301 accgtggtct gccatgcaaa caaccatgaa tcattaactt tgtatgtcac ataaccatca
      361 aactgatgct gatcattacg cgccctgttg ccatctaaat ccgtctgtcg gaaagctttc
      421 cagtcatagc gatagcgtaa ggccagatta agatctggcg ttgcctgata cgaaattttc
      481 agataagggt tatactgact tcctgctgac atccattgtg tctgaatccc tgggattaga
      541 gcccatttgt catctatttt atatgtttta tttatatcta ttttattata atttagtgca
      601 aacaattcat tttcattgtt gtgaattgta ttccaggtat ttgactcaaa acttgcccac
      661 caaccattat tccacccctg actaacctga atcaacgttt cataagcgtg actggcgctt
      721 ctgtatcctc cacgtagatt aagagttaat gcctgagagc acaaaggtaa accaagaaga
      781 atcacagtta atagtggata atattttttc ataaatgcct catcattaaa tatatttatc
      841 acttgtaatc tgatttctca atgaaactta tgttccgcaa gccattccag aagcaggctt
      901 tgccattttt ctatcggata tccatttttt ccggatacac caaatccatg ccctccctta
      961 tcaaaaaaat gagcttctgc atgatgtccc ttatcccgga tagagttaaa atatctcaga
     1021 gagtgaataa ctggaacatc cttatcatct gttgcatgca caataaagac tggtggcata
     1081 tactctgtaa cactattttc aagactggct gaatattcac tttcacctgc atccagaaga
     1141 cgtttccggc atccctgatg agcaatactc ctgtcaagag atataactgg atatatcaga
     1201 gccatccagt caggacgcac agaagtctct tcctcataat ggagatgagt aatatatcca
     1261 gcaagatgac cacctgccga aaaccccata catccaacac tcttaaatgt tctctccgac
     1321 attccggccc agtaagaaat aaagttcagg gcattcttcg catccgcaaa ggaataataa
     1381 ccattaacgg agggcattct gtaagaaaga ataaatgccg tatatccctg acctgctatc
     1441 catctgccta catcaagtcc ctctctttca taaacaatcc tcctgtatcc acctccaggt
     1501 ataatcagca cactatgttg attagataca acaggctcaa caactgtaat aaggggcaca
     1561 atacctgtat atattatgcc atcgtcagta ttctcaaata gcgaattgtc agttatgtaa
     1621 cgctcataat tatcaagaga atatatattc tccctgtaat gaatcatttt tttattccct
     1681 gatagcatcc tttattctcc tgcaacattc ttttttcttg ctccataaaa aggttttcca
     1741 tcgacagtat catattcaag cgcagcttct ggatggatcc atctctgtac acgtgatggc
     1801 atatcaaaac caataaggac aagaacaaca aatgttaaga taaaagcaag agaacacatt
     1861 gctgtaccaa gacttgtatg agacgcaaca actgcaccaa gaataggcgc acatgctcca
     1921 ccaagtgaac ctacattata aataaacccc agtcctgccg cacgctgctc cacattaaaa
     1981 taaccactga ttattttcgg caccagagca ccaatcccct gaccaaaaac ctgctgagta
     2041 aagatcagaa ggccaagtaa ccatacatag tcccggtcca caagaaaaac ggggataata
     2101 acaagctgac cgataagaag tgatataaca tatgcttttc gggttccaag ccagtccccc
     2161 ataaaaccac ctatacaact tccagctgca gagccaaaac ctgccagcat aaatatgaga
     2221 gcaacagttt ctggtgaata ctgcatatca accttaagcc atgtaggaag aaaagcctgc
     2281 agtggccatc cgtagaggaa acagaacata accacaagca tcaacataat gcctgttggc
     2341 catcttttcc ccataccctg atatataaaa ctgataaaaa cagccgcaca gcacaatgcc
     2401 ataactgtga ttaaagcagc gtttgcagtg acgcctgaaa aacaaacata tagacatgca
     2461 aatgcaatac aagacagaag aatatttatg tatttattct tttctctgta cagaatatcc
     2521 accattgttc tgacgggttt attctcttgt tgcctttttt gccagtcatc agactccgga
     2581 agattcttgc gaagataaaa agcaaataat attggaagca tgccgacaaa gaaaagagaa
     2641 cgccagcccc atagtggtac tacaatgcta taaacctgtg cagctaaacc tcccccaata
     2701 gaaaatccag aaattaaaaa accacttgcc ttgtttctga gatgaacagg ccagctttcg
     2761 ataacatatg ttacacttga gccatattca cctgccatac caagcccaat aacaatgcga
     2821 gcaataaaca tcactgcata tccaggagcc agaccacagg caagggttcc gagagagaat
     2881 agcactatac ttaaaaccat tgccatcctg cgccccatct tgtccgataa agcgccaata
     2941 gcaagccccc caaaccatcg ggaaataaaa gcagcggaaa taaggcttgc cgcttctatt
     3001 gtcgtcaagc cgaattcatg ctgaacctct gtaagaacca gagatatcag aacgaaatca
     3061 aaaccatcaa gtaaataacc tgtccatgcc gcaccgaata tcttccactg tctgggtgaa
     3121 agatgcttat accaaacctg ctttttctga atacaggagt ccatattaaa cctcacaata
     3181 atagcggatt tataatacac tggagtatta ctttaagtaa tacaatcccg gtcccaaaga
     3241 gttgcaccaa taagaccaga gtcacttctg aaatatgccg gaaacagcat tacattacaa
     3301 atttcagaat atatggaaag atgtttcgac actttctgaa tatacccatc agcaagtcca
     3361 acacttcctc cgacaacaac aacctgacaa tcaagcagta attttaaact tactattaat
     3421 tcagcaatag tactggcaga acgctctgtc aaaaattcgg cttccttata tccctgtctc
     3481 gctaattcaa aaatatattt ggtgctatat cctgcaagtt cattttttgc tgcccccatg
     3541 atggcccgac cagatgaaac cgcttcaacg catccccgac gaccacatcc acattctgta
     3601 tccgttactc cggacaatat atgcccaaca tgtccggcta atccagaaac acctgttaat
     3661 aattttctgt taactacaat tccccctcca acccccgtag aaacggttat aaaaaccata
     3721 tcacaaattt cttttggcaa aactgtatat tctgcccatg cggctgcctg ggcatcattt
     3781 atcacacttc catttaaacc agttatatct tccattatat ttttcagagg atactctttc
     3841 agccctccaa gattgtctgg attcaaagct gtcaatactc cattattaat aatccctgtt
     3901 gatgctactg caaatcgctc cgctgttccc ttcatttcag aaaccagtag acgtaaagcc
     3961 cctgtcattt cgtcaggaca tctttcatga ggtgttgata tctgtttttt cagcaaaaat
     4021 gaaccgtcat cagatatcaa cgccgctgaa attttcgttc ctcctatatc aacagccagt
     4081 gttatcattc attttaccta tataacaatc ttgcaattat tcagattatt ttcatttttt
     4141 tatttctgtc atgttcttcg agcagtcgct ctgcaagttt tgataataca tccagattct
     4201 tttcctttac cggagaaaaa ggacttctgc atacaggagt atttataatc cccatatagt
     4261 aaagaagagt ttttatacct gccaggacac cactctgaat aagagtatca ataacctggt
     4321 tacaggccac ctgcatttcc ttcgccttaa tcacatcatt atttttgact gcttcaaata
     4381 tttcaagata acgccacccc attatattat atgtactacc gattccccca tctgcacctg
     4441 caattaaccc tgatgcaaat atttcatcgt aaccattata taacacaagc tctggatgat
     4501 tccttttgat ttgttccatc tggaataaat caccggatgt ctgttttaat gcacaaaccc
     4561 cgggtattgt gacaagctca tttatctgat ctaaagaaaa acgcactccg ctaagagccg
     4621 gtatattata aactaccatc ggaagtccat ctgcagaatc aataatcttc ctgtaatgga
     4681 tacaatgctc ttcaaatgaa aaaggataat aaaatggagt aacagcagat atagcatgat
     4741 atccgtattt ttttgcagct tttgcaagta cctcgctttc tgccgtactg attccgccca
     4801 catgcgctat aagtgttagt cttccatctg attcatcggc tactgtttca agaatttttt
     4861 ctcgctctgc aacattctga agaaatgctt ctccagtaga gcctccaaca tacaatccct
     4921 taatattttg ttcgatatta aaacggatca gttttcgaag actctccata tcgatttgct
     4981 gggattggtc ataaggagtg ggtaatgctg atataacccc tttaaattca cattgcattt
     5041 ttaatcacct tcacttaact gcataatggg atacctagta caccatcaag tacaaagatt
     5101 atccaacaga aaagaagcac aatgtgaaga ttatcaaaaa aaagccatca gaaagctgtc
     5161 atgccgctca gttaaggatc ggttgatcga tccagtgtcc agttactatt ctctgcgtaa
     5221 ccctctgcga ctatcttgcc ctggaactga ttacccacag ccctgaagag tccgacactg
     5281 tcacgctaca caaacggcgt cttcctctgg aaatgatggt ctggtgaatt atcagtatgg
     5341 cccatgagcg taaggaacct tcatcagatt gtgaaccggc tggacatcat gctgccaggc
     5401 agtagcgccg tgctccatgc ccgccagcgg ccaggaagcg gagccatacg ctgcgtgttc
     5461 actcaaacag cgcagctcta gcataacacc acgccatatc cgctctggtg cgatctgaat
     5521 ctgctgacca ttgatggcgt gttctgacga acacaggaca cgcaagaaca cgataaagct
     5581 tcactcgcca aatacatagt ggaaaccaga cactttatcc tcaggtaaaa atggtctgtc
     5641 tgatagaact gaccagccat ctactgacgg cagcggcctt cgatacaatg aatctggcac
     5701 taaataagac tggcaatata gccgataatg cgttgaatca gtggctatat cgtatacata
     5761 aaacagacat cattattctg tcttgttgtc caacacataa aaactaataa aaacatacca
     5821 ttccctgtcg tcagagaatt tattgataaa caggctttat tgaacaaatc aaacattcag
     5881 ggaactggca gttgaacaaa acgccagtcc gaagactggc gcagtgataa aacattctgc
     5941 atcatgccac attctgctcc tgaagatgac gcagacgatg gtcccagttt cccaggtaat
     6001 gcccgggagt cagcagtacg cggcgtcgct cattacagag cgagttacca atcaccactt
     6061 ctccggccac accacacaac gacagttgta tatacgccat gccggctgcc agcgggtcaa
     6121 tatccgttgc cgacacccac atcctgtggt atgccggata ccccgaacgg ttcaggatgt
     6181 cagccattgc cagaatcata ctgccggcac cacaggcagg ttcactgagc gtgataaagg
     6241 gtttgttctc aaacaacgcc ttcacatctc ccatctgcat ctgtgccatc atgctggcaa
     6301 tactccaggg cgtgaaaaac tgactccggt atttatcccc cagttccagt tgcatgaaca
     6361 cactccccag aaaatcacag aatccctgct ccagtccctc aatgacatga gcaagcaaat
     6421 gcgcaatgtg gataacatcc tgcttttcat aaccactgac gattcgcaga tacttctgtt
     6481 cccgggcctc actgaactga agccggtttt ccagggcaat cacactacag ctgacaaagt
     6541 cttcaaacac cttatgacgt ttgtgataac aggctgtccg gttaaacaac gaaatgaact
     6601 ccttctgatg gtcagtcatg gtggccatgt tttctccgta aaaacaggga acacatggcc
     6661 cggacagggg aacaggtgtt ccccttcggg ttgaaatgtt gataaaacgg gcatcacgga
     6721 catgataccc ggaggttaat tactgggctg agaccagagg aaccggtaaa ggtgcggctt
     6781 tcgtctgttc ttcag
//
LOCUS       DAZGHF010000196         6768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGHF010000196 DAZGHF010000000
VERSION     DAZGHF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.197"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            120..524
                     /gene="ybgC"
                     /locus_tag="ACHK3A_004577"
     CDS             120..524
                     /gene="ybgC"
                     /locus_tag="ACHK3A_004577"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO8742563.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            521..1213
                     /gene="tolQ"
                     /locus_tag="ACHK3A_004578"
     CDS             521..1213
                     /gene="tolQ"
                     /locus_tag="ACHK3A_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO8742564.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            1217..1645
                     /gene="tolR"
                     /locus_tag="ACHK3A_004579"
     CDS             1217..1645
                     /gene="tolR"
                     /locus_tag="ACHK3A_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO8742565.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            1710..2975
                     /gene="tolA"
                     /locus_tag="ACHK3A_004580"
     CDS             1710..2975
                     /gene="tolA"
                     /locus_tag="ACHK3A_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO8742566.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            3108..4400
                     /gene="tolB"
                     /locus_tag="ACHK3A_004581"
     CDS             3108..4400
                     /gene="tolB"
                     /locus_tag="ACHK3A_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO8742567.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELCVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            4435..4956
                     /gene="pal"
                     /locus_tag="ACHK3A_004582"
     CDS             4435..4956
                     /gene="pal"
                     /locus_tag="ACHK3A_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO8742568.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            4966..5757
                     /gene="cpoB"
                     /locus_tag="ACHK3A_004583"
     CDS             4966..5757
                     /gene="cpoB"
                     /locus_tag="ACHK3A_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO8742569.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     EAIVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            5922..5997
                     /locus_tag="ACHK3A_004584"
     tRNA            5922..5997
                     /locus_tag="ACHK3A_004584"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5955..5957,aa:Lys,seq:ttt)
     gene            6133..6208
                     /locus_tag="ACHK3A_004585"
     tRNA            6133..6208
                     /locus_tag="ACHK3A_004585"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6166..6168,aa:Val,seq:tac)
     gene            6211..6286
                     /locus_tag="ACHK3A_004586"
     tRNA            6211..6286
                     /locus_tag="ACHK3A_004586"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6244..6246,aa:Lys,seq:ttt)
     gene            6338..6413
                     /locus_tag="ACHK3A_004587"
     tRNA            6338..6413
                     /locus_tag="ACHK3A_004587"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6371..6373,aa:Val,seq:tac)
     gene            6417..6492
                     /locus_tag="ACHK3A_004588"
     tRNA            6417..6492
                     /locus_tag="ACHK3A_004588"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6450..6452,aa:Lys,seq:ttt)
     gene            6639..6714
                     /locus_tag="ACHK3A_004589"
     tRNA            6639..6714
                     /locus_tag="ACHK3A_004589"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6672..6674,aa:Lys,seq:ttt)
BASE COUNT         1794 a         1604 c         1847 g         1523 t
ORIGIN      
        1 gctccaggcc cataaatttt tacgctccct taacttgccc tcattcccaa acctcaatcg
       61 cgcgcgtata gtagcagcgt ttaaaagctc taacttttgt tgcattaccg ggatgtaaag
      121 tgaatacaac gctgtttcga tggccggttc gcgtctacta tgaagatacc gatgccggtg
      181 gtgtggtgta ccacgccagt tacgtcgctt tttatgaaag agcacgcaca gagatgctgc
      241 gtcatcatca cttcagtcaa caggcgctga tggctgaacg cgttgccttt gtggtacgta
      301 aaatgacggt ggaatattac gcacctgcgc ggctcgacga tatgctcgaa atacagactg
      361 aaataacatc aatgcgtggc acctctttgg ttttcacgca acgtattgtc aacgccgaga
      421 atactttgct gaatgaagca gaggttctgg ttgtttgcgt tgacccactc aaaatgaagc
      481 ctcgtgcgct tcccaagtct attgtcgcgg agtttaagca gtgactgaca tgaatatcct
      541 tgatttgttc ctgaaggcta gccttctggt taaacttatc atgttgattt tgattggttt
      601 ttcaatcgca tcctgggcca ttattatcca gcggacccgt attcttaacg cagcggcgcg
      661 tgaagccgaa gcgtttgaag ataaattctg gtctggaatc gaactctctc gcctctatca
      721 agagagtcag gggaaacggg ataatctgac tggttcggaa caaatctttt acagcgggtt
      781 caaagagttt gtgcgcctgc atcgtgccaa tagccatgcg ccggaagccg tagtggaagg
      841 ggcgtcgcgt gctatgcgta tctccatgaa ccgtgaactt gaaaatctgg aaacgcacat
      901 tccgttcctc ggtacggttg gctccatcag cccgtatatt ggtctgtttg gtacggtctg
      961 ggggatcatg cacgccttta tcgccctcgg ggcggtaaaa caagcaacac tgcaaatggt
     1021 tgcgcccggt atcgcagaag cgttgattgc gacggcaatt ggtctgttcg ccgcaattcc
     1081 ggcggtaatg gcttataacc gtctcaacca gcgcgtaaac aaactggaac tgaattacga
     1141 caactttatg gaagagttta ccgcgattct gcaccgccag gcgtttaccg ttagcgagag
     1201 caacaagggg taagccatgg ccagagcgcg tggacgaggt cgtcgcgatc tcaagtccga
     1261 aatcaacatt gtaccgttgc tggacgtact gctggtgctg ttgctgatct ttatggcgac
     1321 agcgcccatc atcacccaga gcgtggaggt cgatctgcca gacgctactg aatcacaggc
     1381 ggtgagcagt aacgataatc cgccagtgat tgttgaagtg tctggtattg gtcagtacac
     1441 cgtggtggtt gagaaagatc gcctggagcg tttaccacca gagcaggtgg tggcggaagt
     1501 gtccagccgt ttcaaggcca acccgaaaac ggtctttctg atcggtggcg caaaagatgt
     1561 gccttacgat gaaataatta aagcactgaa cttgttacat agtgcgggtg tgaaatcggt
     1621 tggtttaatg acgcagccta tctaaacatc tgcgtttccc ttgcttgaaa gagagcgggt
     1681 aacaggcgaa cagtttttgg aaaccgagag tgtcaaaggc aaccgaacaa aacgacaagc
     1741 tcaagcgggc gataattatt tcagcagtgc tgcatgtcat cttatttgcg gcgctgatct
     1801 ggagttcgtt cgatgagaat atagaagctt cagccggagg cggcggtggt tcatccatcg
     1861 acgctgtcat ggttgattca ggtgcggtag ttgagcagta caaacgcatg caaagccagg
     1921 aatcaagcgc gaagcgttct gatgagcagc gcaagatgaa ggaacagcag gctgcggaag
     1981 agctgcgtga gaaacaagcg gctgaacagg aacgcctgaa gcaacttgag aaagagcggt
     2041 tagctgctca ggagcagaaa aagcaggctg aagaagccgc aaaacaggcc gagttaaagc
     2101 agaagcaagc ggaagaagcg gcagcgaaag cggcggcaga tgctaaagcg aaggccgaag
     2161 cggatgctaa agctgcggaa gaagctgcga agaaagcggc tgcagacgcg aagaaaaaag
     2221 cagaagcaga agccgccaaa gccgcagccg aagcgcagaa aaaagccgag gcagccgcgg
     2281 cggcactgaa gaagaaagcg gaagcggcag aagcagctgc agctgaagca agaaagaaag
     2341 cggcaactga agctgctgaa aaagccaaag cagaagctga gaagaaagcg gctgctgaaa
     2401 aggctgcagc tgataagaaa gcggcagcag agaaagctgc agccgacaaa aaagcagcag
     2461 aaaaagcggc tgctgaaaag gcagcagctg ataagaaagc agcggcagaa aaagccgccg
     2521 cagacaaaaa agcggcagct gcaaaagcag cagctgaaaa agccgctgca gcaaaagctg
     2581 ccgcagaggc agatgatatt ttcggtgagc taagctctgg taagaatgca ccgaaaacgg
     2641 ggggaggggc gaaagggaac aatgcttcgc cagccgggag tggtaatact aaaaacaatg
     2701 gcgcatcagg ggccgatatc aataactatg ccgggcagat taaatctgct atcgaaagta
     2761 agttctatga cgcatcgtcc tatgcaggca aaacctgtac gctgcgcata aaactggctc
     2821 ccgatggtat gttactggat atcaaacctg aaggtggcga tcccgcactt tgtcaggctg
     2881 cgttggcagc agctaaactt gcgaagatcc cgaaaccacc aagccaggca gtatatgaag
     2941 tgttcaaaaa cgcgccattg gacttcaaac cgtaatcgcg atgttgactg ttccgacggt
     3001 caacatcagg caccggttgc cacggggttc tggtagtttt gtgtatttta gtttgttaac
     3061 attctgctaa attatcgtgg gccatcggtc cagataaggg agatatgatg aagcaggcat
     3121 tacgagtagc atttggtttt ctcatactgt gggcatcagt tctgcatgct gaagtccgca
     3181 ttgtgatcga cagcggtgta gattccggtc gtcctattgg agttgttcct ttccagtggg
     3241 cgggccctgg tgcggcacct gaagatattg gcggcatcgt tgctgccgac ttgcgtaaca
     3301 gcggtaaatt taatccgtta gatcgcgctc gtctgccaca gcagccgggt agtgcgcagg
     3361 aagtacaacc agctgcatgg tccgcgctgg gtattgacgc tgtagttgtc ggtcaggtca
     3421 ctccgaatcc ggatggttct tacaatgttg cttatcaact tgttgacact ggcggcgcac
     3481 cgggtactgt acttgctcag aactcgtaca aagtgaacaa gcagtggctg cgttatgctg
     3541 gtcataccgc cagtgatgaa gtgtttgaaa aactgaccgg cattaaaggt gcgttccgta
     3601 cccgtattgc ctacgttgtt cagaccaacg gcggtcagtt cccgtatgaa ctgtgcgtat
     3661 ctgactatga cggttacaac cagtttgtcg ttcaccgttc accgcagccg ctgatgtctc
     3721 cggcgtggtc accagacggt tctaaactgg cttatgtgac cttcgaaagc ggtcgttccg
     3781 cgctggtcat ccaaacgctg gcaaatggcg ctgtacgtca ggtggcttca ttcccgcgtc
     3841 acaacggtgc acctgcattc tcgccagacg gcagcaaact ggcattcgcc ttgtcgaaaa
     3901 ccggtagtct gaacctgtac gtaatggatt tggcttctgg tcagatccgc caggtgacgg
     3961 atggtcgcag taacaatacc gaaccgacct ggttcccgga cagccagaac ctggcattta
     4021 cttctgacca ggccggtcgt ccacaggttt ataaagtgaa tatcaacggc ggtgcgccac
     4081 aacgtattac ctgggaaggt tcgcagaacc aggatgcgga tgtcagcagc gacggtaaat
     4141 ttatggtaat ggtcagctcc aatggtgggc agcagcacat tgccaaacaa gatctggcaa
     4201 cgggaggcgt acaagttctg tcgtccacgt tcctggatga aacgccaagt ctggcaccta
     4261 acggcactat ggtaatctac agctcttctc aggggatggg atccgtgctg aatttggttt
     4321 ctacagatgg gcgtttcaaa gcgcgtcttc cggcaactga tggacaggtc aaattccctg
     4381 cctggtcgcc gtatctgtga taataattaa ttgaatagta aaggaatcat tgaaatgcaa
     4441 ctgaacaaag tgctgaaagg gctgatgatt gctctgcctg ttatggcaat tgcggcatgt
     4501 tcttccaata agaacgccag caatgacggc agcgaaggca tgctgggtgc cggcactggt
     4561 atggatgcga acggcggcaa cggcaacatg tcttccgaag agcaggctcg tctgcaaatg
     4621 caacagctgc agcagaacaa catcgtttac ttcgatctgg acaagtacga tatccgttct
     4681 gacttcgctc aaatgctgga tgcacatgca aacttcctgc gtagcaaccc gtcttacaaa
     4741 gtcaccgtag aaggtcacgc ggacgaacgt ggtactccgg aatacaacat ctccctgggt
     4801 gaacgtcgtg cgaacgccgt taagatgtac ctgcagggta aaggcgtttc tgcagaccag
     4861 atctccatcg tttcttacgg taaagaaaaa cctgcagtac tgggtcatga cgaagcggca
     4921 tactccaaaa accgtcgtgc ggtactggtt tactaagaga attgcatgag cagtaacttc
     4981 agacatcaac tattgagtct gtcgttactg gttggtatag cggccccctg ggccgctttt
     5041 gctcaggcac caatcagtag tgtcggctca ggctcggtcg aagaccgcgt cactcaactt
     5101 gagcgtattt caaatgctca cagccagctt ttaacccaac tccagcaaca actctctgat
     5161 aatcaatccg atattgattc cctgcgcggt cagattcagg aaaatcagta tcaactgaat
     5221 caggtcgtgg agcggcaaaa gcagatcctg ttgcagatcg atagcctcag cagcggtggt
     5281 gcagcggcgc aatcaaccag cggtgatcaa agcggtgcgg cggcatcaac gacgccgaca
     5341 gctgatgctg gtactgcgaa tgctggcgcg ccggtgaaaa gcggcgatgc aaacacggat
     5401 tacaatgcag caattgcgct ggtgcaggat aaatcccgcc aggatgaagc aatagtggca
     5461 tttcagaatt tcatcaaaaa ttaccctgat tcaacttacc tgccaaacgc caattattgg
     5521 ctgggtcagt taaactacaa caagggtaaa aaagatgatg cggcgtatta ttttgcttcg
     5581 gtagtgaaaa actatccgaa gtcaccgaag gctgcagatg cgatgtttaa agtcggcgtc
     5641 atcatgcagg acaaaggtga caccgcaaaa gcgaaagccg tataccagca ggttatcagt
     5701 aaataccctg gtactgatgg cgctaaacag gcgcaaaaac gtctgaacgc gatgtaatgc
     5761 ataacacacg accagaagtc gcattatttc tggtcgtgtc gtgcgaatca taagcagttg
     5821 agtgatctgc atcgaaattt ttgttgcgct caagtctgaa atcagtaata tatgccgccg
     5881 ttgccacggg atatcaaaca aaccgaaagc aacgaaaaag tgggtcgtta gctcagttgg
     5941 tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatc
     6001 gctaaggtgg aagcggtagt aaaacgtgaa ggataacgtt gcgtcagcaa cggcccgaag
     6061 ggcgagacga agtcgagtca tcctgcacga cccaccacta acctagttag ttgtagtatc
     6121 cagcgtagta tcgggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc
     6181 ggcggttcga tcccgtcatc acccaccacc gggtcgttag ctcagttggt agagcagttg
     6241 acttttaatc aattggtcgc aggttcgaat cctgcacgac ccaccactaa ttacggtggg
     6301 ttcggtggaa gtagtttgta gtattcagcg cagtatcggg tgattagctc agctgggaga
     6361 gcacctccct tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccactcgggt
     6421 cgttagctca gttggtagag cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg
     6481 cacgacccac cagttttaac atcaaactca gatgttaagc gtgaaggata acgttgcgcc
     6541 agcaacggcc cgtagggcga gcgaagcgag tcatcctgca cgacccacca attttaaaga
     6601 ttggttccga gtagaaatct ttcaggtaac acccgtatgg gtcgttagct cagttggtag
     6661 agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accatcctga
     6721 atgattaaag cagcataatc ccgcaagggg tcgttagctc agttggta
//
LOCUS       DAZGHF010000197         6739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGHF010000197 DAZGHF010000000
VERSION     DAZGHF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.198"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            81..1415
                     /gene="adeQ"
                     /locus_tag="ACHK3A_004590"
                     /gene_synonym="yicO"
     CDS             81..1415
                     /gene="adeQ"
                     /locus_tag="ACHK3A_004590"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO8742570.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISSVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1468..1920
                     /gene="yicN"
                     /locus_tag="ACHK3A_004591"
     CDS             1468..1920
                     /gene="yicN"
                     /locus_tag="ACHK3A_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO8742571.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2131..3321
                     /gene="nepI"
                     /locus_tag="ACHK3A_004592"
     CDS             2131..3321
                     /gene="nepI"
                     /locus_tag="ACHK3A_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO8742572.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3362..3655)
                     /gene="yicS"
                     /locus_tag="ACHK3A_004593"
     CDS             complement(3362..3655)
                     /gene="yicS"
                     /locus_tag="ACHK3A_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO8742573.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3877..4695
                     /gene="nlpA"
                     /locus_tag="ACHK3A_004594"
     CDS             3877..4695
                     /gene="nlpA"
                     /locus_tag="ACHK3A_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO8742574.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4699..5622)
                     /gene="yicL"
                     /locus_tag="ACHK3A_004595"
     CDS             complement(4699..5622)
                     /gene="yicL"
                     /locus_tag="ACHK3A_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO8742575.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5789..6286)
                     /locus_tag="ACHK3A_004596"
     CDS             complement(5789..6286)
                     /locus_tag="ACHK3A_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742576.1"
                     /translation="MGHGTCKAAKSARHLIESKEKNRSSRVKVFSTHINLSHPAASWL
                     ATQTCANNIFNLLQNNNVIYLFENDNKIELRMGYDSFFSIETIDKINKENIKEIKLKN
                     EKTYVSLPNPTILITTTSTPERQFSISVLDDDNKENLPTDTAPCFTIGIPDEYTKAIE
                     LLNIK"
BASE COUNT         1670 a         1453 c         1679 g         1937 t
ORIGIN      
        1 atgtgataat tatcactgaa ccgttgttgt catttgtttt acaaaagcaa gggattaaat
       61 ctcctccaat ggacaaaaaa atgaataatg acaataccga ttacgtgagt aatgaatcag
      121 ggacgctttc gcgattattt aaactacctc agcatgggac caccgtccgc acagaattga
      181 ttgcggggat gaccactttt ttaaccatgg tatacatcgt ttttgtgaat ccgcaaatcc
      241 tcggcgcggc acaaatggac ccgaaagtgg tgtttgttac cacctgtttg attgccggta
      301 tcggcagtat tgcgatgggg atatttgcta acttacccgt ggcgctggct ccggcaatgg
      361 ggctgaacgc cttctttgcg ttcgtggtcg tgggggcgat gggcatctcc tggcagaccg
      421 ggatgggcgc gatattctgg ggcgcagttg ggctatttct gctcacgctg tttcgtatcc
      481 ggtactggat gatctccaac attccattaa gtttacgtat tggtatcacc agcggaatcg
      541 gattatttat cgccttaatg ggattaaaaa atactggcgt tattgtcgcc aataaagaca
      601 ccctggtgat gattggcgat ttaagttctc acggcgtgtt gttaggtatt ttagggtttt
      661 ttattataac cgtgttgtca tcacgtcatt ttcatgccgc ggtgcttgtt tctattgtgg
      721 tgacgtcttg ctgtggatta tttttcggtg atgttcattt tagcggcgtc tattccattc
      781 cgcctgatat tagcagcgtc attggtgaag tggatttgag cggcgcgtta tcacttgaac
      841 tcgccggtat cattttctcc tttatgctga tcaacctatt tgattcatca ggaacattaa
      901 ttggtgtaac tgataaagct ggcttaatag atagtaacgg taaattccct aatatgaata
      961 aggcgctgta tgttgatagc gtcagttcgg tggcgggtgc gtttatcggc acctcgtctg
     1021 ttaccgccta tattgaaagt acttctggtg tggcagtcgg tggtcgcacg gggctgactg
     1081 cggttgtggt cggcgttatg ttcctgttgg ttatgttctt ctcaccgctg gtggcgatgg
     1141 ttcctcctta cgcaaccgcc ggagcgttaa tctttgttgg cgtgttgatg acctcaagtc
     1201 tggcgcgcgt taactgggat gattttaccg aatcggtacc tgcgtttatt accacggtga
     1261 tgatgccctt tactttctcg atcaccgaag ggattgcact cggctttatg tcgtactgca
     1321 tcatgaaagt gtgcaccggg cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag
     1381 ctctgtttgc actgaagatt attctggtgg attagtaact ggcgagggtt tccagatatc
     1441 atgagttctg attacgcagg agaactcatg atctggataa tgctcgccac gctggcggta
     1501 gtgtttgtgg ttggttttcg ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc
     1561 agcgatcggc tgaacatcga tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag
     1621 tcagccggtg acgaattttt acgttatttg catcgtccgg atgagtcgca cctgcaaaac
     1681 gccgcgcaag tgttgctcat ctggcaaatt gtcattgtcg atggtagcga acagaacttg
     1741 ctgcaatggc atcggatttt acaaaaagcc cgccttgccg ctcccattac cgatgctcag
     1801 gtcaggctgg cgctgggttt tctgcgcgaa accgaacctg aaatgcagga tattaatgct
     1861 tttcagatgc gctataacgc gttctttcag cctgccgagg gcgttcactg gctgcattaa
     1921 aaccgcttcg tcttatcagc ctacagaccg cgcctgacct gtagggtgga taaggcgttc
     1981 acgccgcatc cggtaatctt tgccctgttc cgtttcgatc ttaactccag ctcaagcaac
     2041 accgtaaaag ttgcaaaaac gttaaatacg tcacacattt caatgttaaa gtggtcggct
     2101 ttccctctga aacatgccac gggtaacacc atgagtgaat ttattgctga aaaccgcggc
     2161 gcggatgcca tcacccgacc gaactggtca gccgttttct cggtggcgtt ttgcgtcgcc
     2221 tgtctgatta tcgttgagtt tttgcctgtc agtttgttga cgccaatggc ccaggattta
     2281 ggcatttcgg aaggggttgc cgggcaatcg gtgaccgtga ccgcctttgt ggcgatgttt
     2341 gccagtttgt ttattaccca gaccattcag gccactgacc gccgctacgt tgtcattctg
     2401 ttcgccgttt tgctgacgct ttcctgtttg ctggtttcct ttgccaactc attcagtctg
     2461 ctgttaatcg gtcgcgcctg tctggggctg gcgctgggcg ggttctgggc gatgtcggcg
     2521 tcgctgacca tgcgtctggt accgccgcgt acggtgccga aggcgctgtc ggtgatcttc
     2581 ggcgcggttt ctattgcgct ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt
     2641 atcggttggc gcaatgtctt taatgcggcg gcggcgatgg gtgtgctgtg tattttctgg
     2701 attatcaaat cactgccttc gctgccaggt gaaccttcgc atcagaaaca aaatactttc
     2761 cgcttattac aacgtccggg agtgatggcc gggatgatcg ccatctttat gtcctttgcc
     2821 ggacagtttg cttttttcac ttatattcgc ccagtgtata tgaatctggc gggattcggt
     2881 gttgatggct taaccctggt gctgctgagt tttggtatcg ccagctttgt cgggacatcg
     2941 ctttcgtcgt ttattctcaa acgttcggtg aaactggcct tagcgggcgc accgtttgtg
     3001 ctcgccctga gcgcgctggt actgacgctg tggggaagcg ataaaattgt tgctactggt
     3061 gtggcgatta tctgggggct gacctttgct ttaattcccg ttggttggtc aacgtggatc
     3121 actcgctcat tggccgatca ggcagaaaag gctggatcta ttcaggtggc cgttattcag
     3181 cttgccaata cctgcggtgc ggcaatcggc ggttatgcgt tggataacat tggtctgact
     3241 tcgccgctga tgttgtccgg cacattgatg ttgctgactg cattgttggt gactgcaaag
     3301 gtgaaaatga agaaatcctg atatcaggta aagaagcgtt ggtcagtggt tgttgccgtt
     3361 cttacatatc cgggcattct accttaccca acgcttccca ataattgctc tgattattac
     3421 gttccatcgc cacgatggcc tcgcgtatat gttgtttatt gttttcatct gacaacatta
     3481 acttttccca cgcttcatct gataatgacg cctgtggcgt gcaaatttta cgtaaatctt
     3541 gcaataccgt ggttttctct tttgccgact gtagagaact gaatggcgac tcggcctgag
     3601 aaatggctgg aaaaatcagg aatacggtaa tcatgagtag catcgttggc ttcatagcgg
     3661 caaccttctg caaatgtgca tcgaatgaag cataagtgta gtttgctttt gccgctctca
     3721 tcatgctgta cgaaaggtgt ttgcgtggtc agtaagaagt gccagacttt atattccact
     3781 tttattcctt tttattctta tcgatagcgt ttcgtttttt aaactgcagc gaccttaccg
     3841 ctatagtcag gtaatcatta ataaaaggat aaaaaaatga aactgacaac acatcatcta
     3901 cgggcggggg ccgcattatt actggcagga attctgctgg caggttgcga ccagagcagc
     3961 agcgatgaaa agcacattaa agtgggcgtt attaatggcg cagaacaaga tgtcgcggaa
     4021 gtcgctaaaa aggtggcaaa agagaaatat ggcctcgatg ttgaactggt aggatttagc
     4081 ggttcgttgc taccgaacga tgcgacaaat catggcgagc ttgatgcgaa cgtcttccag
     4141 catcgaccct ttcttgaaca agataatcag gcgcatggtt ataaactggt agcggtggga
     4201 aatacgtttg tcttccccat ggcgggttat tccaaaaaaa tcaaaaccgt ggcgcaaata
     4261 aaagaaggcg cgacagtggc gatccccaac gaccctacca accttggtcg cgcgctttta
     4321 ctgttgcaaa aagagaagct aattactctg aaagagggaa aagggttact acctaccgcg
     4381 ctggatataa ccgataatcc acgccatttg cagataatgg agctggaagg ggcacagttg
     4441 ccacgtgtac tggacgaccc gaaagtggat gtagcgatta tcagcaccac ttacatccag
     4501 caaacaggac tttctccggt taacgacagc gtatttattg aagataaaaa ttcgccgtat
     4561 gtgaatattc tggtggcacg ggaagataat aagaatgcag aaaacgtgaa ggaatttctg
     4621 caatcttatc aatcacccga agtcgctaaa gcggcagaaa ccatctttaa cggaggcgcg
     4681 gtgcctggct ggtaattctc actcatgccg cgccggacga tttatttttc tggcgcggcg
     4741 gcgggaatcc attgaaatca aaatcaccga tgacagaatc agcagcgttc ccagccagtc
     4801 cggcaatgtg aaggtgatcc ccagcaacag caaagagagc agcgcgctac ttaatggttc
     4861 tgcacagctt aaaatgctgg cttttggacc gccaattaat tgtgctcctt tcaggtacag
     4921 actaaatgtc agggacgtac caatgaccac caaataaaaa aacgccagaa tcaaactgcc
     4981 gttaacgaca aagtttgtcc cttgtctggc ataaaagggc agcaggatca gaccgccaat
     5041 cagcatactc cagccgacga ccggtaacgt gccatagcgg gcaatcagcg ttgagggata
     5101 ggtggtatag aatgcagcag caaatgccga ggcaatgccc cagaataacg cggcaggaga
     5161 gatcgataat gacgtcggat taccgtgtgt caccaataaa aaagtcccga tcagcgatgt
     5221 caaaatagcg cagaaaacca gaatgcccgg gcgggattta cgcaccagtg agaaccaggc
     5281 gacgataata gtcggtgaga ggaattgcag caccgttgcc gtggctgcgt tcgatttttc
     5341 tatggttagc aaaaaagtga gctgtacggt tagcgcgcca accacggaaa aaatcagtag
     5401 gctaatggca tctttgtgat tgttaataat agaaaagatt ttatcaccat gaacaaatga
     5461 tagcgtcagc agaatcaaac cggcgaatat caaacgcgtc atagtcaaaa actgcgacga
     5521 catctggctt tgctccatga tgtattgcgc gcagacccct gaacttcccc acaacacggc
     5581 ggcaatcaga acgttcagca tcccctttct ggtggaaccc atttttccct ctgcatactg
     5641 ttttgtcttt tcatcaatat acgttaagga taccactacc gatgacggtg agggagtaga
     5701 gtaatcaatc agttttagtg aatggaggaa ataatgacat ataacaatta ttcatatggt
     5761 atttaaaaaa actcaatatg aaatttaatt atttaatgtt caataactct atggctttag
     5821 tatattcatc aggaatacca attgtaaaac aaggtgcggt atcggtaggt agattttctt
     5881 tgttatcatc atccagaaca gaaatagaaa actgtcgttc aggtgttgat gtcgtggtta
     5941 ttaaaatggt tggattagga agagaaacat atgttttctc attttttagt tttatttctt
     6001 tgatgttttc tttgttaatt ttatcaatcg tttcaataga aaaaaaagaa tcatacccca
     6061 ttcgcagttc aattttatta tcgttttcga ataaataaat gacattattg ttttgaagta
     6121 gattgaatat attgttagcg caagtttgag ttgctagcca ggatgccgct ggatgactca
     6181 aattaatatg cgtgctaaag actttgacgc gactacttct gtttttctct ttactttcaa
     6241 ttaaatgcct agcgcttttg gctgctttac aagttccatg ccccacacgg caaaaataat
     6301 ctcctgaaag gatacttaca atcagtatta caccaatgac gacagaaaaa atagttccga
     6361 tgagtttgat gatgacaatc cttgtgcagt gttattttca ccatggatta taatgttata
     6421 gtgaaattat tgcacatgta atttttcttt gattgtttta acatcacatt tatgcacttc
     6481 ttcatctttg tagcaggtaa aagccatata tggatggctg gtgtccggtg cagataaaat
     6541 gcaacttatt cttgatagct tgaaaaaaag agaggttttt ttgagtgacg gttgatagtc
     6601 ggtggcaagg aagacgtttt cgtacactcg gcaagaaaaa tgaatcactc tgtcatacta
     6661 ccagatacat cagcagggtc aggttgctca gaaataagca ggatggggat cactgaagag
     6721 gcattttggc gatcacagg
//
LOCUS       DAZGHF010000198         6734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGHF010000198 DAZGHF010000000
VERSION     DAZGHF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.199"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(267..2552)
                     /locus_tag="ACHK3A_004597"
     CDS             complement(267..2552)
                     /locus_tag="ACHK3A_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8742577.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAITGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELCDAWVDA
                     HGEKESLFTDEAQTHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIRITRTTDWRIVPKVPVVQPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKYGLRTPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSTVMARI"
     gene            complement(2542..2817)
                     /locus_tag="ACHK3A_004598"
     CDS             complement(2542..2817)
                     /locus_tag="ACHK3A_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO8742578.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(2814..3038)
                     /locus_tag="ACHK3A_004599"
     CDS             complement(2814..3038)
                     /locus_tag="ACHK3A_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO8742579.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGVSALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRMA"
     gene            complement(3038..3340)
                     /locus_tag="ACHK3A_004600"
     CDS             complement(3038..3340)
                     /locus_tag="ACHK3A_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO8742580.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKKLNDKRSW"
     gene            complement(3340..3564)
                     /locus_tag="ACHK3A_004601"
     CDS             complement(3340..3564)
                     /locus_tag="ACHK3A_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFO8742581.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3628..4128)
                     /locus_tag="ACHK3A_004602"
     CDS             complement(3628..4128)
                     /locus_tag="ACHK3A_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFO8742582.1"
                     /translation="MTVMTLNLVEKQPATMRRIIGKHLAVPRWQETCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4125..4295)
                     /locus_tag="ACHK3A_004603"
     CDS             complement(4125..4295)
                     /locus_tag="ACHK3A_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742583.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(4306..4581)
                     /locus_tag="ACHK3A_004604"
     CDS             complement(4306..4581)
                     /locus_tag="ACHK3A_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HFO8742584.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            4703..5002
                     /locus_tag="ACHK3A_004605"
     CDS             4703..5002
                     /locus_tag="ACHK3A_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8742585.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            5118..6131
                     /locus_tag="ACHK3A_004606"
     CDS             5118..6131
                     /locus_tag="ACHK3A_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO8742586.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(6408..6725)
                     /gene="yegR"
                     /locus_tag="ACHK3A_004607"
     CDS             complement(6408..6725)
                     /gene="yegR"
                     /locus_tag="ACHK3A_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO8742587.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAVHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         1780 a         1719 c         1479 g         1756 t
ORIGIN      
        1 cctgaagctt caaaactatc atttacgttt gcgaggcacc atctatcaca ttgatttata
       61 ataagtatct ttatcttcac tgcattgctc tcaaaaaatc acatagatgg aaattccccg
      121 ttgacacact cccaagtgat gcagatacat ttcaagtgct tcatacacat gataacggct
      181 atccccctaa attctgtgcg tttcttttcc ctccagcaca aggttgaaat atcttgcgtg
      241 cagctttact ttgtgaatct ttgtcattat atccgcgcca ttactgttga gaatcccggc
      301 cactcatcag cgaccgggta cgtgaatttt ttcccgtcat aatttacggt cgcgccacgc
      361 gccagcgcct caagctccca tcgctgcggc ctgataccgt tctgagcaag gtcaacgcgg
      421 atacgggtaa tttgcattct ttccgaccgg gtcagtctgg ccgatggtgc aatttcatgt
      481 ggttttaacg ggcttccgtt tctctgctga cgatttgggg ttctcagccc gtattttaat
      541 gcgcccctga gcgccctcac gacctccggg tcattccatt cgataacacc gtcatcaacc
      601 agattaagca ctgctgcggc gtgttcagaa ggtgtgggag ccggtaacga agtatcacca
      661 ccggtgagct ttccacagtt attgacagga ctccgaggcg cggcgatgcc gctttttaaa
      721 gtcaaaggct gaacgaccgg aactttcggc acaatgcgcc agtccgtcgt tctggtgata
      781 cgaatatgac gcgcgccgag atgcggcgcg taaatgccga ccactctctc gacctcttcc
      841 tcgtactcgt taacgtcatc cgaggggcta cgggcgaccc tgacagtctg gcaatcgcgc
      901 gggacatttg ccccaccctg cgcgctgata tacagcgcaa aatcgccact gtctgcggca
      961 gcgcgtgcag cctcgacgcg ttcgtcaaac tcatcagcaa tgctgacgcc gcgaggcaat
     1021 ttgcgtagtt cacggtaagc ccccattgtc ggcaggccaa ccgttttaaa ttgaggaatg
     1081 cgccacgttg acgcccatgc ggtaacagcc gcggcagtgt ctttcagcgg cctgccggtg
     1141 tcgttatcga gctgaccatc cagtgcatag ccatcgatgt tttttgaaat gtatttcgcg
     1201 atatatcccg cagcaccgcc ccggttaagg tgttttgcct gaaaacggtt tcgcgcggct
     1261 cctctttcgt cgccatcctc tttgagcgcg tagcgacgca tgatttcgat aatctgatta
     1321 cgctggcgtg gattacaaaa aagcatcata tgccagtgcg gcgttccgtc gtggtgtggc
     1381 tcgacgacac gcaaaccgta gacctgtaaa tcattatcct tgaatgccgt gcgcatcagg
     1441 ctccagatgc ggcagagata acgctgcgca tcctttggat taaatgcctc atcgttccag
     1501 ccgtgattaa gctgcacggt tttactttcg ccttttccga cctgacgtgt cgggtgatac
     1561 tttgacggtg cggtcagcgt gataaacatc cccacatcgc cctctgcggc ggcgtaacgc
     1621 tcaataccgg caatggtgtt catcagctcc atccggcgaa tttcaggatt agaaatactg
     1681 cccatcacct tactgataag gtcgatgcgc tcgccggttt ccctgttttc gaggtcacac
     1741 gatttaagaa attccagatt tgcctggcgg cgcgcacgca catcacgaat ggcatgttta
     1801 ctggcataag gagaacggtc tttattgacc tccccgacag caatcagtaa cgcctcatgc
     1861 cagcgcatac gctggccttt aagctgacta atccaccact catcgttaaa cagacgggca
     1921 atggcagaat atgcctgcct cgtggtcatc tgccctttac ggtatttttt ccagtagagc
     1981 ggggaaatat tgaaagcacg tgcagcgcca gcgacatgac catacaggtg agtctgcgcc
     2041 tcatccgtaa acagcgattc tttttcgcca tgtgcatcca cccaggcatc gcagagttcc
     2101 tcatacatca tgaaaagctg cgatgagata cgggcggcaa actttttcag ctccttgtca
     2161 ttcattccag gcaggcgcgc ataatggtca cgctctgcca gaaacagcaa cgacgcgtcg
     2221 gtgttcattt catggcgctg attcacgcgc tcaatgcgcg gccataaacg acgctgaaaa
     2281 gtggatgtga ggaaataaaa cccgtgcacc gggcttttat tgcgccggat gtagtcataa
     2341 cgtgaagtaa acagcgagcg caaaaagtaa ggcaggcggt taatcgtgga taaaacacct
     2401 tgcacctgac gcatctcgtc acgtgtaagg ggtctttcgc gcccgacagc ctcgcgtggc
     2461 gcgttccatg cataagcacc ggtaatcgcc ttaccggtgc ctgcggcaaa tgctgacgga
     2521 gggacaaaac gcccggaggc tttaacggcc atatgagcca aaagcctcag aacaacgctt
     2581 gctgagttgc tcaacctgcg cgtttaaatc agcaaaagac tttgcgcttc cggtcagaat
     2641 atcgtgatgc atcaggccgg aaacgagctg gcttaatttc gggtaataac caaccaccgc
     2701 cagccattcc tgcccggcgt ttttaccgct ttccgctctc tttttctcgt ggagaataaa
     2761 ctgaaagctg tcactggtaa cgacataacg ttcgccaatt tcgatacgaa tactcatgcc
     2821 attctccggt aatgcttgtt ttttgcttca aagactgact ggcaggaaac acaacgcgtg
     2881 gctgacggat aagctgcacg acgggcagca ggtattggcg cgtcacactc ttcgcaaacc
     2941 agcgcagaaa caccgcaatg ttttaccctt gccgcgttaa tctggcgctc cagtaattca
     3001 gcctgttgtt cctgaataaa atctatgttg tccggcatta ccagctcctt ttgtcgttca
     3061 gcttcttaaa ttcatcagcg caatagctgg cgatttctgt cgttaatttt gtcagttcat
     3121 ccacggatga aatttgcttg tggaatacag cacgtttaac aagtaaattg accacatcag
     3181 acaggaggtt taattcattc tgataaatcg cgataacaga ttcagttatg tcgcgctttt
     3241 ctttatcaag acaaagttga ataagagaca aatcaccatt ttccataacg gcgattttta
     3301 aggcgttatt cagtaataca actgaatgag aacaggacat caaagtacct ccccgcgaga
     3361 caatccgata ttgtgaaatt tttccgactc ctgactgagc agctcgacta tctccacgcg
     3421 ggataactcc gcctttgtga tgtggcgaat catggcgtca agatgagaag aaaagcgcgt
     3481 agctgcatct gcctgtgctt cggttctggc ctgttgcagc agtaatgcgt atttaccgca
     3541 ctgattttca gaaactgtat gcatgacttt ctccaggcaa aaagaagccc cgcacaatta
     3601 agtgcgttaa aaactctggt taattactta atgcagatat tgctctggtt ttaccgatgt
     3661 cagaattgtc ggtgcatact caaacagact gaataattca cgtaatgcac ggaataaagc
     3721 atcacgccag taacatgatt cttcattaat tcgccagtac ggctggttga attctttttc
     3781 tgtcagtcgt gcgtgcataa ataaagtacg acgctggctg actgttaaaa aactaatata
     3841 tgcatactca cttgcaccaa cctgacggcg ttttgagaat gccccacgca attcatcaat
     3901 tgcacatacc agtcgttcac gttcgacgtc gttcatttct tcaaaacgca tcgttgcgtg
     3961 acgctgtttt aactgcgcat gaaagcaaac cgttagccgt tcgcgttcca tcatctgatt
     4021 ataataatcg catgtctcct gccagcgagg gacggccaga tgcttaccaa ttatccggcg
     4081 catagttgct ggctgttttt caacgagatt gagcgtcatc actgtcattt ccataccctc
     4141 cggcttttca gaaaggtcag agcctttttt aacggactct gttttttggt gcggataatg
     4201 attcccttac gccccttacc gtgggtgatg gtgaagtcaa tcgccctggg gctttcgtta
     4261 cgcaataact gagcaataca acgaggctca ttcatacggt tctccttaac gtggttcacc
     4321 gagacctaac cacatcaacc agccgtcacg aatctcttta ggacggcttt cataagccag
     4381 ttttagtccg ttattccatg ccggaaggta tacccaatat tcaccagcac gccccgatac
     4441 tgactgaggg tcagtaatct caataactgg taatttccct ttctcaatca taccccttac
     4501 agctcttgga gttttaccaa tgagttttgc aaactcctga taaggcacgg catcagtcac
     4561 gcttacaagc tgtctattca tctgctacga ttctccctta gtgcttctaa tggctcctaa
     4621 tggctaatta ttgcctaaaa ggataactcc agaagcacaa catttcacac tatcagcaag
     4681 aaattacgca atcggagtaa ttatgtcaat agacgtttcg gagaagttga agctaatccg
     4741 tgaatctgaa aggttaaacc gtaaagaatt cagtgaatta actggtgtag cctacagctc
     4801 actttcgagc tatgagagcc ggtcaaaaaa cgctggagtt gaagccataa tgaaggtctt
     4861 acaacatcct agatttacta aatatacttt gtggttcatg actgatcagg tagctccaga
     4921 agccgggcaa attgcgcccg ctctcgcaca ctttgggcaa aacgaaacaa cgtcgcccca
     4981 ctccggtcaa aagactggtt aacaatttat cgtgaatata ttcatcacaa gtgcctacta
     5041 ttggtggcta aatttcagcc accacgaaaa aagcgattag tagtagcaaa aaaaagtacc
     5101 actcggaggg ttttctgatg gcaatcaaaa aactcgatga tggtcgatat gaagtggaca
     5161 tccgccctac tggacgtaac ggaaaacgca tccgtaggaa gtttgataag aaaagcgaag
     5221 ctgtcgcttt cgaaaaatac acgttgtaca accaccacaa taaagaatgg ctatcaaaac
     5281 caacagacaa acgacgtctg tcggaactga cacagatctg gtgggattta aagggtaaac
     5341 acgaagagca tgggaaatct aatcttggaa aaattgaaat cttcacaaaa ataacgaatg
     5401 acccatgcgc atttcaaatc acgaaatccc ttatcagcca gtactgcgcc acccgaagaa
     5461 gtcagggtat taaaccttcg agtatcaatc gtgatttaac atgtattagc ggcatgttta
     5521 cagccctgat tgaagcggag ttattctttg gtgagcaccc tatcagaggg acaaagaggc
     5581 ttaaggagga aaaaccagaa acaggctatc tcacacagga agaaattgcc ttactgcttg
     5641 ctgctcttga cggcgacaac aaaaagattg cgattctttg cctgagcact ggagcacgtt
     5701 ggggagaagc agctcgtttg aaagcagaaa atatcatcca taaccgcgtc acgtttgtta
     5761 aaacgaaaac aaacaaacca cgcaccgtcc cgatctcaga ggctgttgcc aaaatgatcg
     5821 cggataacaa acgaggtttt ttattccctg atgctgatta ccctcgcttc agacgaacaa
     5881 tgaaagcaat aaaaccggat ttgccaatgg ggcaagccac acatgcacta aggcacagct
     5941 ttgccactca tttcatgatt aatggaggaa gtattatcac gctacaacgg atactaggtc
     6001 acacgcggat tgagcaaact atggtttacg ctcattttgc gccagagtac cttcaggacg
     6061 ccatttctct taatccgcta agaggtggta ctgaggccga gagtgtccac acagtgtcca
     6121 cagtagagta acgtttaagg gctttcagtg gtaatttatg ccgctcaaac ccgcattgta
     6181 ccgttgaaag cccctactgg tgacacccta aatctccctt acacgggctt attttttata
     6241 cataaaccat agagacggat tgatcccagg tcaccgtctt ccattgacca catcgataga
     6301 atctcccttc atagcacgat gcctttcact tattggcatc gtgctcgcac aggttccggt
     6361 tacgcacagc cagaacgcgc atgtttgatg cttaccaaaa aatattctca ctctccacat
     6421 ttgaatgtca gacgagcgac gccatgtaat cctgcacctt ctgtcttcag gtcaactatc
     6481 tgcatttttt tgccctgagt aacacagaaa tgaactgcat cattttttac tatattttct
     6541 gcaccagata ttctacccct ggctaaagaa gcttcggctt cggtgtagta ttggttatcg
     6601 agtttacgct gaatattact tttatatgca agaccaaatt taccgatact tgtctcatca
     6661 ttatgtacag cacacccaga cataagaaaa acactaatta atgatatagc agctatcttt
     6721 ttcatctcac cttc
//
LOCUS       DAZGHF010000199         6715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGHF010000199 DAZGHF010000000
VERSION     DAZGHF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.200"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..278)
                     /locus_tag="ACHK3A_004608"
     CDS             complement(<1..278)
                     /locus_tag="ACHK3A_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO8742588.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMP"
     gene            complement(455..1366)
                     /gene="gsiD"
                     /locus_tag="ACHK3A_004609"
     CDS             complement(455..1366)
                     /gene="gsiD"
                     /locus_tag="ACHK3A_004609"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO8742589.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGASIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1369..2289)
                     /gene="gsiC"
                     /locus_tag="ACHK3A_004610"
     CDS             complement(1369..2289)
                     /gene="gsiC"
                     /locus_tag="ACHK3A_004610"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO8742590.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVGDEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2307..3845)
                     /gene="gsiB"
                     /locus_tag="ACHK3A_004611"
     CDS             complement(2307..3845)
                     /gene="gsiB"
                     /locus_tag="ACHK3A_004611"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO8742591.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3865..5736)
                     /gene="gsiA"
                     /locus_tag="ACHK3A_004612"
     CDS             complement(3865..5736)
                     /gene="gsiA"
                     /locus_tag="ACHK3A_004612"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO8742592.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     SGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYRGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVVAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5723..6688)
                     /gene="iaaA"
                     /locus_tag="ACHK3A_004613"
     CDS             complement(5723..6688)
                     /gene="iaaA"
                     /locus_tag="ACHK3A_004613"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO8742593.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     AMVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
BASE COUNT         1671 a         1946 c         1593 g         1505 t
ORIGIN      
        1 ggcatcattt ccattttcag aattacggcg cgttgaatct cctctgtatt ttttaataaa
       61 tatagtgagt cctttaatgt cagggccgaa cgttcaatat ctttaaagaa atagccaacg
      121 tttttctgaa taagttcaaa gcggctatcg gcacgtttta ccgcgcgttc cgataccagt
      181 tgatagttaa tgatgaaaaa aagaccaata aatgacagtg ctgccaataa aaataaaatt
      241 atcagcctta tctttatctt ttcgtataaa ctcagcatgt taatacattt aacaaaataa
      301 ctgtctgaac gctgtgccgc tgcaaaatag tcccttgctg cggcttttta accaaattac
      361 ccgattctac aaaatcagca gaaagttaca acacttgctt agggcaataa tttcctgtga
      421 ggtgattacc ctttcaagca atattcaaac gtaactatcc tttaattttc ggatccagcg
      481 catcgcgtaa accatcgccc aacaaattga acgccagtac ggtcagaaaa atagccaggg
      541 ccggaaaaac agcgacatgc ggcgcgataa ccatatccgc tcgtgcctca ttgagcattg
      601 caccccactc tggtgtcggc ggctgcgcac cgaggccgag aaatgacagg ctggcggcag
      661 agataatcga ggcgccaatg cgcatggtga aaaacaccac gatagaagag acggtcccag
      721 gcaagatatg acgcaacaaa atggtcatat cgctggcacc aatactgcgt gctgactcga
      781 taaaggtttg ctgtttcaac accagcgtgt tgccgcgcac cagccgggca aacgcgggga
      841 tggaaaaaat ggcgactgcg ataatcacgt tagcaatgcc gcttcccaac accgcaacaa
      901 cagcgatcgc cagtaaaata cccgggaagg caaacaacac atcgcaaatg cgcatgatca
      961 gccgatccca ccagccttca taatatccag ccagcaagcc cagcaacgtc ccgatcgccg
     1021 caccgataaa cacggcaaac acgcccgccg ccagcgatat ttgcgcacca accaggacac
     1081 ggctgaaaat gtcgcgcccc agtgaatcga caccaaacca gtgctgcaaa gaaggtccgt
     1141 tattcagatt gtcataatca aaataatttt cggcgtcata gggagcgatc cagcgtgcaa
     1201 aaatggccac cacaatcaat aaaataacga acaatgcggc ggtcatcgcc atatgctgac
     1261 ggcgaaatcg tcgccagaat tcatgccacg gtgtacgtac ctggtcaggt ttgaccagtg
     1321 gcatggcgtt taacaccgcc tgacgtcgcc agttaaatag tcgcatcctt acttgtacct
     1381 gatagccggg ttaatggcgg cgtaaagcac atccaccact aagttgataa gaataaattc
     1441 cagcgagaaa agcagaattt ccgcctgaat caccgggtaa tcgcgcattt caacggagtc
     1501 aacgagtaag cgtccaagtc cgggccagtt gaaaactttc tccaccacga tggaaccacc
     1561 gagtaaaaat ccaaactgta agcccatcat ggtcactacc gggatcatcg cgttgcgtag
     1621 cccgtgtttg aggacaaccc aggtttcgct cacccctttc gccctcgcgg tacgcatata
     1681 atcttcgctt aaaacgtcga caaacgacgc gcgggtaaag cgcgccatca cggcggccac
     1741 tgccgcgccg agcgtcaggg agggtaaaat gtagtgctgc caactgtctg ctcccacggt
     1801 aggcagccag cccagttcaa cagagaatac ctgaattaaa agcatcccca gagcaaatgc
     1861 cggaaacgaa atccccgaca ccgcaatagt catactcaat cgatccggcc aacggttacg
     1921 ccagacggcg gcgataattc ccgccgccat accaaatatc accgcccaga ccatactggt
     1981 tatggtcagc cacagcgttg gcataaagcg gctggcaatc tcatcgccaa ccggacgacg
     2041 cgacaccatc gacaggccaa aatccccctg cacagcattg ctgatatagt gccagaactg
     2101 gtgatacagc ggctgatcca accccagctg ctgacgcacc agttctataa cctgcgcatc
     2161 agcttcgggc ccggcaatta atcgcgccgg atcgccgggc agcatgtgga caaataaaaa
     2221 caccagcacc gagacgataa acagcgtcgg aatcaacccc agtaagcgtt tgataacgta
     2281 attaagcatt ccactccctg cgttgcttat tgcaaatccg catcttcaaa gctgaagccg
     2341 gtgtctggca tgatccaaaa accggtcagg tttttactgt gtgccgacac aagtttttct
     2401 accaccagcg ggatccacgg cgactcttgc cagatgatat cctgcgccgc cttatacaag
     2461 cgggtctttt ccgccggatc attagttttc agtgcctgag ccaggaagtc atccacctgt
     2521 ttattgctgt aaaacgcggt attaaacagc gttggtggcc agttctgcga ggcaaacagc
     2581 ggcgatagag cccagtcggc ttcgccggtt gaagccgacc agccagtgta gaacatccgc
     2641 acgccgctct ctttttgccc tttaccttca acttctgccg cccgctgtcc ggcatccatc
     2701 gcagtcacct gggctttaat cccaacctgc gctaactgct gctgggtaaa ttgcagcact
     2761 ttctgcgcgg tgctgtggtt atgtgacgac cacagcgtgg tactgaaacc gttgggatat
     2821 cccgcctctt tcagtaattc gcgcgctttc actggatcgt aaggccacgg tttataactt
     2881 tgcgcgtagg cgatactcgg cggtaccaca ccagtagctg gcgttgcata gcccgcgaag
     2941 gccactttca ccaacgccgg gcggttaatg gcgtaattca gcgcctcacg gaccttcggg
     3001 ttatcgaacg gcttttgcgt cacgttcata ctgatataac gctgcataat tgacggactg
     3061 gccatcaact cgatattttt gtttttctcc agcagtgcgg cctgctcgta gggaatgggg
     3121 aaagcaaact gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt atccgccacc
     3181 ggacgccagg ttatgctgtc cagtttgggc aatcctggct gccagtaacc cgcgaatttt
     3241 ttcaccttca caaaatcggt ctgattccag gtatccagtt catacggccc ggttcccacc
     3301 ggatgaaaac caatctcctt gccatatttt tccagcgctg ccggtgaaat catcgcggtc
     3361 gccggatggg caagaatatt aataaacgct gagaatggct gtttgagggt aatctttacc
     3421 gttgtcggat cgatcgcttc cgttttggca atattcttat acaggttata gcgtttaaga
     3481 tgattcgccg gatcgctggc ccggtccaga ttcgctttta ccgccgctgc gttgaagtcg
     3541 gttccatcct ggaatttaat gccttcccgc agtttcacgg tgtaagtaag gccgtcatcg
     3601 gaaacggtat aactctccgc cagcacgttt ttcagtttca tctctttatc cagaccgaac
     3661 agcccctggt aaaacgattt cgctacggcc tgagataacg tgtcattcgc gtcatatgga
     3721 tcgagcgtgg tgaagttcga tcctaccgcc accaccacat ctttggcagc gaatgcacaa
     3781 gatgccatca acgctgtcgc aatgcccagc gccactaacc cactacggtg tacagctctt
     3841 gccattttat tctccgcctg aatgttatct acgcatgaat gcgtattctg attgtggttg
     3901 tgcgacgtaa tgccccggcc cgacgcattg caacgagacg gctacaacct cttcaccacg
     3961 cagatgaata ttgctgggaa gatcgtccga cagcagtaca cgctgcggtc gttgtcggga
     4021 cggttcagcg accggaactg ccgccagtaa tttacgcgta taaggatgct gcgggttttc
     4081 gaagaccgcg cgccgtggac caatttcaac aatttgtccg cgatacatca ccgccacacg
     4141 atgactaatc cgctctacca cggccatatc gtgggagata aacagatacg caatgccgaa
     4201 atcacgctgg agatcgagca acaagttgat aatctgcccg cgaatagaaa catccagcgc
     4261 cgagacggct tcgtcggcaa tgatcacttt tggattcaat gccaacgcgc gagcaatgca
     4321 gatgcgctgg cgctgaccgc cggaaaactc atgagggtaa cgccaggcat gttccggtaa
     4381 caggcccacg cgctccagca accacgcaac gcgtgcagcc gcttctttac ctggcaataa
     4441 accgtgtacg cgcagcggtt cgataatcga atcaccgatg gtctgacgtg ggtccagcga
     4501 agcgtaaggg tcctgaaaaa taaactgaat atcccggcgc aaagcctgaa gtttaccgga
     4561 tgacaaggta tcgattcgct gaccgttaaa gataatttcg ccgccctgcg attcgaccag
     4621 gcgcagcaac gcccgcccgg tagtggattt accgctgcca gactcgccca ccagcgatag
     4681 cgtttcgcca ggccagagat caaaactgac tttctcaacg gcatgcactt cccgcgttac
     4741 gcgattcaac aaaccgctgc gcaaagggaa acgggtgacc agattacgca cccgtaaaac
     4801 aggttcgcca tccaccaccg ttttctgctc gatggggggt tcctgttttg ctggatgttc
     4861 aagcgatatc aatgggaaac gtcggggata atctaaccct ttcatcgcac caagttgcgg
     4921 aacagcagct aacagcgcac gggtataagg atgttgcggt gcatgaaaaa tctgttcgac
     4981 gctacccgtt tccaccgcct cgccctgata catcaccagt acccgatcgg caatctctgc
     5041 caccacgccc atatcgtgag tgataaagat aacgcccatc gacatctctt tttgcaatac
     5101 tttgattaat tgcaggatct gcgcctgaat agtgacatcc aacgcggtgg ttggctcatc
     5161 ggcaatcagc accgccgggc ggcatgacag cgccatcgca atcatcactc gctggcgcat
     5221 cccgccagag agttgatgcg gataacgtga aaggatggtt tgtgcctccg gaatgcgtac
     5281 ctgatccagc atccgcttcg cctcgaccat cgcttcttca cgactggcgt tctgatgcag
     5341 acgaattgat tcggcaatct gttcacccac agtaaatacc gggttcagcg atgtcatcgg
     5401 ttcctgaaat atcatcgcca tatccgcacc gcgcacatgg cgcatttgtg cagcgctctg
     5461 ctcgctaagt tcaatcactt cacgactgcg ccgccgcaac agcatttgat cgcactgcac
     5521 taaaccgccc gcctgttcca acaagcgcat caatgccagc gcagtcactg acttaccaga
     5581 gccggattcg ccaacaattg ccagcgtctc accgcgttgc aggctaaaag agagattgcg
     5641 gaccgcagct attttctgct ggtcctgcat aaaggcaata ttcagatttt caaccgccag
     5701 cacatcaccg gcatcaagtt catcactgtg tggcaacggt gtcccctttt tcacggtaga
     5761 taccggtggt tggcgtatcg cctgcgtagc cccaggcgcg atacattcct tcggtgttaa
     5821 acggtagcgc gacgttacct tcatggtcga tagcgattaa gccaccgcta ccgccaagcg
     5881 caggaagttt ttccattact acccgctcgc aggcttccgc gagacttaat ccgccgtaat
     5941 ccattaacgc ggcgatgtca tatgccgcca gcgcgcggat gaagacttcg cccgtgccgg
     6001 tacaagaaac cgccacactg gcgttattgg cgtagcatcc ggcacccact aaggggctat
     6061 cgccaactcg tccgggtaat ttattggtca ttccgcccgt ggacgtagct gccgccagat
     6121 tgccgtctaa atccaacgcc acggccccca cggtgcccat tttctgtttt tcatccagtg
     6181 gcgcaccgct atggtcgaga accattgccc cttcctcgcg cgctgccagt agttgttcat
     6241 aacgcaaagg cgtggagaaa atctccggcg agacgcgctc catgccatga gcaaacgcaa
     6301 aattttctgc cccttcgcca atcatcatca catgcgggct ttgctccatc accagccggg
     6361 cggcaagaac cggattacgc agatgactaa cgcctgccac cgcaccggct ttcagggtgt
     6421 taccatccat cacacaggcg tccagttcat gggtttcatc acgcgtaaag acagcgccaa
     6481 ttccggcgtt aaacagtgga cactcttcca gcagacgcac cgcttccgtc accacatcca
     6541 gcgcactttc gcccgcttcc agcattttct gcccggtttc aacaatggca gacagcgcct
     6601 cgatgtagcg taattcctgt tgcagactca tctgcgcgcg gctaattgca cctgcgccgc
     6661 catgaattgc aatgactgct ttgcccatag tattcgtcca ttaagggtgg ctcgc
//
LOCUS       DAZGHF010000200         6651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGHF010000200 DAZGHF010000000
VERSION     DAZGHF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.201"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..522
                     /locus_tag="ACHK3A_004614"
     CDS             <1..522
                     /locus_tag="ACHK3A_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742594.1"
                     /translation="SYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTR
                     PDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAIN
                     SELYTKMIYLTRNWSLYPNGDGCVTISSPEIKSKYPAAICLILGFFLSIVISVMFCLV
                     KKMVDEYQQNSGQ"
     gene            complement(763..1458)
                     /locus_tag="ACHK3A_004615"
     CDS             complement(763..1458)
                     /locus_tag="ACHK3A_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO8742595.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERFTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1451..2878)
                     /locus_tag="ACHK3A_004616"
     CDS             complement(1451..2878)
                     /locus_tag="ACHK3A_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO8742596.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2889..3608)
                     /locus_tag="ACHK3A_004617"
     CDS             complement(2889..3608)
                     /locus_tag="ACHK3A_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO8742597.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGLKVKVMHIAEVLMSR"
     gene            complement(4136..4990)
                     /gene="rclR"
                     /locus_tag="ACHK3A_004618"
     CDS             complement(4136..4990)
                     /gene="rclR"
                     /locus_tag="ACHK3A_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO8742598.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5216..6541
                     /gene="rclA"
                     /locus_tag="ACHK3A_004619"
     CDS             5216..6541
                     /gene="rclA"
                     /locus_tag="ACHK3A_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO8742599.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADIPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1816 a         1638 c         1437 g         1760 t
ORIGIN      
        1 agttacagtc taaaaataaa ccagacaaca ccaggcatac ttgttagctg tgatagcaat
       61 aataattttg cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt
      121 tttcagacca gaccagatgt caagaacaga gaaataaagc tggaatggtc aggagataag
      181 agagatttac caactgctga agcggaaata tctcgcgtgc aggcgtctat tatcaaatgg
      241 tatgcgtcag aataccataa tggcaggcaa gttcttgatg agatacaaac gccttcagca
      301 attaacagtg agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg
      361 aacggtgatg gctgtgtaac cataagctca ccggaaataa aaagtaaata ccctgctgcc
      421 atttgtctga ttctgggatt ttttctaagc attgtgattt ctgtaatgtt ttgccttgtc
      481 aaaaaaatgg tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgacag
      541 agagtgcctg tataaaacta tagtgacaat gaccgggaag ggagcccgtc ttcaggaatg
      601 gattcctgtc tttttcactg taaatatcct ggcattatta atgtcaggat atttacagaa
      661 gagcagcata accatgacgg aacggagtta agtcaggttg tcattagcgc tattcgcaac
      721 ctcgtgccgc cttcaggttt atgtccagac ttcatatctc tctcagcaat cctcaataat
      781 cagatacacc gctttcaccg gaccatgaac cccgacgact ttgataagct caatgtccgc
      841 cgttgaactg ggaccactga tgatgttaat acatgacggc attcgttcac cggcctgcgc
      901 tttctgatgc aatttttctg cgagttgcgc tacacgcggc aggattgtgc ttttacgcag
      961 gataaaaaga gaagattccg ggagcaggct caatgaacgc ccgcgcccgg cggcggaaaa
     1021 aagaaccacg cctcccgatt cggttaaacc atattcagca tatacgacgc ccactttagc
     1081 ctgctctgct tgtgatatgt tctcaatacc tttcgccgga tcccaaacaa cggcattgca
     1141 ttcctgctgc aaacgttcgc taatccccaa ttcctccaac ctcgtgtcac cactaatcac
     1201 gaccgactga tctcccagct ctttacacag acgtattgca gcttctgccg ccttcgcctc
     1261 gctggtcagt tcacagcgcg tcaacataac atcgctggca aactggataa acgcgtcaca
     1321 gcgctgctgt tgggtaagtt gggtaaaccg ctcgttagca tagttgttaa gcggcgcatc
     1381 ttctgcttgc ggttcaagtc gcagcgggcg acccagtgcc tgagcaacgt tattcaaaaa
     1441 ttcgctccga ttatccattc tttttctcct gcgcctgatg tttcttaaac caactacgga
     1501 aactctctcc gtcagcttca ggaagatcgc gtgcttccat ccagtcgcta atcgcgccaa
     1561 atttgagtgg tgttttgccg ccattgataa accagcttgc cgcatgagca ccagccatca
     1621 tcccaacttt ccacaatcct ggatgactat tggcataagc gaacattttt atcgcccgtt
     1681 gctctgcttt tgcggtgatc cctttttcag ccatcacccg acgatgacgc aaaatcagtt
     1741 ttgacagcgg aatacgcacc ggacacacgc tgtcacaagc ggtgcataaa gagcaggcgt
     1801 agggtaaatc tttaaaatct ttatagccgc caagtaacgg ggaaatcacc gcaccaattg
     1861 gccctggata aatagagcca tatccatgac cgccaatatg gcgatatgcc ggacaagtat
     1921 tcatacaagc cccgcaacga atacagcgca gcacatcccg aaattcagag gccagcacct
     1981 cagaacgccc gttatcgaca ataaccagat gaaactcttc aggaccatca acgtgccccg
     2041 cttcgcgcgg tcctgtcagc caggtgttgt atcccgtcaa acgtgcacca acggcactgc
     2101 gcgccagcat ggtgatcaat acatctacct ctgcaaacgt gggagcaata cgctccattc
     2161 ccatcactgc aatatgcgtt ttaggcagcg tggtacacat tcgcgcatta ccttcattgg
     2221 tcaccaggca taccgaaccg gtctctgcca ccgcgaaatt acagccggta atacctattt
     2281 cagcactgag gaaatcttcg cggatttttt gccgaatgaa taaggtcatc gcttcaggcg
     2341 tttccggccc ctcatagccc agacgttcgt gtagcacccg acggatctga tggcgatctt
     2401 tatgaattgc cgggaccaca acatgagagg gcggatcttg atccagctgg agaatgtatt
     2461 cacccagatc ggtttcaatc acctgaatgc cagcatcctg caacacatga ttgacaccaa
     2521 tctcttcggt caccatcgat ttagatttca ccaccttccg ggcatttttg cgttgggcaa
     2581 cctgtaaaat gtagcgggta gcgtcttctt tggtttttgc aaaatagacg tgaccgccgt
     2641 tttgcgtcac tttttctgag agctggtaca gataagcgtc gagattactc agaacatgat
     2701 cacgtatctg ggcggcccga tcgcgccact cctcccagtg ccccaattca tcgaccattt
     2761 tttgccgatt tgccccgata cgctgctgcg cgtttgccac cgctttgcgc atgatcggat
     2821 cttcaatttg ctgacggatg cgtgtcttaa aatctgtatt actggttttg atcgacatct
     2881 ttatatcctc agcggctcat caacacttca gcaatatgca tcactttgac tttcagccct
     2941 tcccgttgta atcgtccact gatgttcagc aggcaactca cgtcagcacc tattaaatac
     3001 tcagggcgga cttccatcag gtgcgcaacc ttttctttca ccatctcgcc ggatatttcg
     3061 gccattttga ccgagaacgt gccgccaaat ccgcagcagg tatcctgttc agcaaaggtc
     3121 aacagctcca gtccacgcac atttttcagc agcgtaagtg gttcgtcctt cacccccagc
     3181 ttacgggcca ggctacaaga tgggtgatac accgctctcc cttgcaaact ggcacctaca
     3241 tcgactaccc ctaatgtatt aacaataaaa gaggtgagat cctgcatacg cgcggcaacc
     3301 ttttcggcac gtgatgccca ttcaggttca tccgccagat acgtcgggta actttttacg
     3361 gcataggtgc aagagccagc tggtgaaata atgggatcgt cgttatcctc cagtgcggcg
     3421 atcagatttt tcatccctgg aatcgcttct ttgatataac cactattgat cgcaggctga
     3481 ccgcagcatc cctgtttctc cgggaaattt acgcgacagc cgagtttttc cagtagcagc
     3541 acggagtctc gtgccattct tgatttcagg gcgtcaccaa tacaggtgac aaagaaattg
     3601 acattcacaa ctaatactcc attacttcat gcccatttat gcccgatggt tccccccttc
     3661 cgggggatca atcatcaata aatatggcgt tcttttttaa ttggctagca acataccacc
     3721 cctcatttgt atgacaaatg ataatctttc atccaaactt caaatgcaat aactaatttt
     3781 atttctttcg gcatcgctca cattttatag ggaaatcgac gatcattttt ggcataagaa
     3841 atccatttaa aaaggctaca tcccttatac ctattcagtg ctacttattc aggggttatt
     3901 gagatataca ctgatggtgt acaaacaaaa tcaccaacaa taattacgta tcaagtaata
     3961 gtcggcattt tatttaatta aagcaattta gtttgcgttt ttatttaaaa actgagctag
     4021 agtccatcaa aaaaaatata taaagcccaa aaccaccgtt ggaaatagaa atagttgaaa
     4081 ggctcacaac tcccggaatg cggattatct ttaccctgat ttctggcggt tttattcagg
     4141 gtgcaagctg tctcaccctt tcccgatatt ctcccggagt acaaccaaac tcgcggacaa
     4201 acgccttgtg aaaagatgat tcactggcat agcctactga ctcagcgatc accacaacag
     4261 ggagcatttc ccgggaaaac atctgggccg ctatttgtag acgcaacttt gttaatacag
     4321 ccagcggcgt ggttccggaa acatcacgga aaagctgggc aaaacttgcc cgggacatgt
     4381 gggcgatgct ggccagcgat tcgacggtcc aggcgtgtcc tggcatttcc agcatttgct
     4441 gtattaccgc accaagacgt ggatgcagaa gcaaactgag aatgtttttc tctgtattaa
     4501 cctgtgcaat ccactcacgc accgcgaggg taaagaatgt agcgcagatc tggctacaca
     4561 gagcatccac tcccggcatt gccgatctgg attcctgttg taaaaacggg atcgcctccc
     4621 tcagccagtt atattccacg ctatggttca ctggtgctac aaacagcgtt tccggcaaag
     4681 atgttaaaaa ataacgcgcc gaatgttgca gccgaagagt gccacagaca atacaggtcg
     4741 attcattatc gacatgactc aggcgatgag ctgaattttg tggtagcagg accacatttc
     4801 ccgggcgtaa tgtaaaaatc tcccccgtcg gcatttccag cttcgccgct ccttgcgtta
     4861 acgcatgcca acgaataacc gacaattccc cggcaccatg tggaagttgc cagtcacttc
     4921 ctaacacgca attcttatcg atcgttcctt gtggagcgtt aagcatcaac aaacggctaa
     4981 gggcatccat atcagactcc tgagtggttt ttgattacaa acaccaacaa tcttagacaa
     5041 taattaatct aatatcatca aatggttaat catgattttt gttttgtgat tctgattaag
     5101 aaaaagaaaa taccagacga taagacaaaa tatcaagcgt tgtgaagaaa tgttatttgc
     5161 tcttttgcgt ctatagtcat gatgtcaaat gaacgcgttt cgacaggaaa tcatcatgaa
     5221 taaatatcag gcagttatta ttggttttgg caaggctgga aaaacattag ccgtcacgct
     5281 ggcaaaagca ggttggcgtg tggctctcat cgaacaatca aatgcaatgt atggcgggac
     5341 ctgtattaat atcggctgta tcccaaccaa aacattggtt catgacgcac agcagcacac
     5401 agattttgtc cgtgccatac agcgtaaaaa tgaagtggtt aattttttac gtaataagaa
     5461 ttttcataat cttgcggata tacccaatat cgacgtgatc gacggccagg cggagtttat
     5521 caataatcat agcctgcgtg ttcatcggcc tgggggaaat ctggagattc atggcgagaa
     5581 aatttttatt aataccggtg cacaagccgt ggttccgcca attcctggaa ttaccaccac
     5641 gcctggtgta tatgacagca ccggattact taatctaaaa gaattgcctg ggcatttagg
     5701 tattttgggc ggcggatata ttggcgttga gttcgcctct atgttcgcta attttggcag
     5761 caaagtaacc attttagagg cagcttcgct gtttttgcct cgggaagatc gggatattgc
     5821 tgataatatc gcgacgattt tacgcgatca gggtgtcgat ataatcctca atgcccatgt
     5881 ggagcgaatc agtcaccatg aaaatcaagt gcaagtgcat agcgagcacg cccaactggc
     5941 ggtggatgca ctgttaatag cttccggtcg tcaaccagct accgcttcgt tacatccaga
     6001 aaatgccggt atcgcagtaa acgagcgcgg ggcaattgtc gttgacaagc gattacatac
     6061 caccgcagac aatatttggg cgatgggaga tgttaccggc gggctgcaat ttacttacat
     6121 atcactagat gattaccgca ttgtacgtga tgagttactg ggtgaaggca aacgtagtac
     6181 tgatgatcgg aaaaatgtgc cttattccgt atttatgaca ccgcccctgt ccagggttgg
     6241 tatgacagaa gaacaagcca gagagagtgg tgctgatatt caggtggtga cattgcctgt
     6301 agctgcaatt ccgcgcgcca gagtgatgaa cgatacccgt ggggtattaa aagcgattgt
     6361 tgataataaa acccaacgta tattaggggc atcactgcta tgtgttgact cccacgagat
     6421 gatcaatata gtgaaaatgg tgatggatgc cgggctgcct tatagcatat tacgcgatca
     6481 gatatttact catccgtcga tgagcgaatc actcaatgat ctattttcat tagtcaaata
     6541 aactcaaaat cagacgccag aacaaatatc ctggcgtctc agagaaaaga atcttattaa
     6601 ttctctgtta ctctataaat tcttaatcac ttcattgatg gtattttata t
//
LOCUS       DAZGHF010000201         6606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGHF010000201 DAZGHF010000000
VERSION     DAZGHF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.202"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..479)
                     /locus_tag="ACHK3A_004620"
     CDS             complement(<1..479)
                     /locus_tag="ACHK3A_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8742600.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQLSELTAMTASFVLATPTETDG
                     "
     gene            complement(479..820)
                     /locus_tag="ACHK3A_004621"
     CDS             complement(479..820)
                     /locus_tag="ACHK3A_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8742601.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVMTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVN"
     gene            complement(813..4055)
                     /locus_tag="ACHK3A_004622"
     CDS             complement(813..4055)
                     /locus_tag="ACHK3A_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8742602.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAITQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESAHQYALT
                     IDEIAHKTPSMSLPEASDNEGRTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGNGFMINHLTSVKTVTEGLAQATEQFAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSQLVMNGQHTEFNRLLGLGNELLQQRQGLASVPLRLPQATLD
                     DKQQSALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRGGKSETEKAEASFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREETLKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGRMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(4119..4532)
                     /locus_tag="ACHK3A_004623"
     CDS             complement(4119..4532)
                     /locus_tag="ACHK3A_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742603.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLEKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
     gene            complement(4596..4805)
                     /locus_tag="ACHK3A_004624"
     CDS             complement(4596..4805)
                     /locus_tag="ACHK3A_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO8742604.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEADPLAALEDALLFASEN"
     gene            complement(4901..5275)
                     /locus_tag="ACHK3A_004625"
     CDS             complement(4901..5275)
                     /locus_tag="ACHK3A_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO8742605.1"
                     /translation="MKKELKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(5290..6006)
                     /locus_tag="ACHK3A_004626"
     CDS             complement(5290..6006)
                     /locus_tag="ACHK3A_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO8742606.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(6072..6416)
                     /locus_tag="ACHK3A_004627"
     CDS             complement(6072..6416)
                     /locus_tag="ACHK3A_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO8742607.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(6413..>6606)
                     /locus_tag="ACHK3A_004628"
     CDS             complement(6413..>6606)
                     /locus_tag="ACHK3A_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO8742608.1"
                     /translation="GNSDNTMKADNPRNAFYWRFVEMGTVNMPPHPFVRPAFDVRSEQ
                     AAQVAIARMNRAIDEVLRR"
BASE COUNT         1379 a         2163 c         1531 g         1533 t
ORIGIN      
        1 ccgtccgtct ccgtcggtgt cgccagcaca aacgaggccg tcatggccgt cagctctgat
       61 aactgctcca ccacccaccg gtccgtcagc tcctgctccg ggtctgcctc aggattgcct
      121 gccacaaaat tcaccgcatc cagaaaacgc gcatacaccc ggcgacggac caccgtggca
      181 cccaccaggc tctgcaaatc ctccgccatc ccggtgacca gcccgaaaag attggagacc
      241 gtcagcgacg ggcgggcact gctgcccttt ccgttcatct caaagccgct gccctcaatc
      301 gggtacgcct gatattcacg cccctgccag gtcaccggct cccctttttc attcagctca
      361 ttgcagaaaa aataccgctc accgccctgc accgtcaggt cgatttccca gagcaccacc
      421 cgcggtgact gctctgactt aaccgactcg ttcaggcttt cttcgtgaat atcctgcatc
      481 agttcaccac ctgctctatc gtgcaactga aatcactgta ccgggcgtta tccgtaatac
      541 tccactcccg gcataccacc ctgaccgtcc ggttatgttt cgggggcttc cacaaaaaag
      601 cccggtaacc gccatgccat gacagaaacg cggacagtgc ctcccgctca gcatccgttg
      661 tcacccgaaa aaccacctga aaggtcttca gttgcgcatt cagtcctgcc ggacgacgct
      721 gctgataacc gtcgccaaac ttcaccgtca tcaccgacgg tttctccgtc acctgcatcc
      781 cttcccgggg acaccagtga agggtcttaa tctcatccac tcagcattcc tccgtcacga
      841 cgcatggata acatcactgc ctgtaccctc tggtctatca gttgcacaag ggtgcccgcc
      901 gcttccggcc ctatctgccc gttagcgccg tcattctgaa tggcgatgtg gtacaccggg
      961 gaatacacca gacccgcact gccgttcatg cggcccaccg cacgcacgcc gagtgaacca
     1021 tccgctgccc gggtcagggg cattatggct tcaggtccgg cctcccccat cagccctgcc
     1081 ccttttgcaa aggcaaagta tgtgggcgtg tccacaatac tgttactgta tgcactcagg
     1141 tttgccgagg tataaacgcc gccttttgca tttgccaccg cgccacccag ccagttgccg
     1201 atactgccga aaaatcctcc tgcaccggac atactgtttg ccgccatctt aatgccgttg
     1261 acaatggccg cattcataag gacttttgat atttcctgca ggattgatgc agcccagctg
     1321 cgccattcca ctttatttcc gttcagcatc tccgtgatgt tattcaccat ccctgagata
     1381 ccgtctgtcg caagctgtgc tgcctgtgaa gcgtaatctg atgcgctgtc cacccagtta
     1441 cttagccctt cctgcagccc tttctgccag tcagcacgct gcgcatccga ttcggcataa
     1501 aaagcctcct ggtctttaag gcgttcactc agatactgcg cattctgcgc cagcgcctgt
     1561 ctgtaaaaat cctcactgat atcccctgtc tgatactgag actgcaggtc cgcatccttc
     1621 tggcggtagc cgtcacgaat ctgctgcagc tcccgcatcc gttcacggac ccgctcaccc
     1681 tgcccgtacc ccagcagctc agcgtcattt gatgcccttt cgtctgcatt atcatttttc
     1741 agggtctcct cccgggcccg taactgctcc cggattttct tctggtcaat cagggcagca
     1801 ttgcgcagca gctcctgctt ctgtatctcc gtcagggttt tcagctctcc ctgcgaggtc
     1861 tggtacttca gcttcgccag ttctgtattc ttcccggcca gtgccagttg ttccttctgc
     1921 tgcttcagca gacgggaaaa actggcttcc gctttttccg tctcagattt tccgccccgg
     1981 gatttgggtt tattcgcctc attattgcgc caggcttcca tcgaattacg gatgtaacgc
     2041 tgcctcgcct cctgatacgt atcccccacc agaccaaggt catccgccgc ataccccagc
     2101 cgggcacgct ctttttcttc ccctttcagc cgggacaggg ccagttgacg ctctgtgtta
     2161 ttcagggcgc tctgctgttt atcatccaga gtggcctgtg gcagtcgcag cggtacactc
     2221 gccagtccct gacgctgttg cagcagttca ttacccagcc ccagcaggcg gttgaattcc
     2281 gtatgctgac cgttcatgac cagctgtgac tgatacgctt tattctgctc tgccgcctgc
     2341 tgacgaatta acacaacacg gcgctcttcc agtccggcaa gcacatcctg aatggactgc
     2401 gctttttcct gcatctgtgc cagacgggac tgctcaacag caaactgctc tgttgcctga
     2461 gcaagccctt ccgttacagt tttcaccgat gtcagatggt ttatcatgaa gccgttaccg
     2521 gtcgtccagc ccggattagc cagcacatac tgatacccgg cgattttttc ctgcagggat
     2581 ttcacccggc tggcctgctc atcaatcagc cggttctgct ctgtcagcgc ctcccgtgtt
     2641 cgcccttcat tatctgaggc ttcaggcaga gacattgatg gcgttttatg cgcgatttca
     2701 tcaatcgtca gtgcatactg gtgcgcagac tccctggcct gttcctgatt ctggtacagc
     2761 gtgtaccatg cggcagctcc cagcatcacc agtccgggta cgcctccaac cagtcccagc
     2821 gcaccgctca tcagacgtga gcccaccgcc gttgtactgt tcagcgcatt ctgggcggcg
     2881 gttctggcag caatatttct gttcaggcgt tcctgtgtga tcgccagacg ggcctctgcc
     2941 gcaatctgca tctcagtccc gcgggctgcc gccacagcct gctgagcccg gtacacggct
     3001 gcccttgccc gcgccgtggc aatctgcgtt ccccttaact gggcctcagc cagtgccact
     3061 tcattacgtg cagcggtcac aagtcctgcc gtggcagaca tcgctccgga agccagatta
     3121 ccaaagtacc gggccacccc gacagcaacc agcacacccg cagctgttgc cacggtatca
     3181 atatttccgg ccacaccgtt cagtgcgccg gagagcgttt tcgtcgctcc gctggcctca
     3241 ttcgcgccgc ccacccaggc cataaaggcg ttttccacct tcgtgatccc gtcagacacc
     3301 gtttccggca tggctgcata ttcatcacgc aatatcccca gctggctgat taacgcggga
     3361 acgactttat ccgccgtcag ttgaccatcg tccgccatcg ccttcagatc cttacgggcc
     3421 acgcccatgc ctgcagccag tgcgcgaatg atccggtccc cactttcatt gaccgaatta
     3481 aattcctcgc cgcgcaacac cccctgtgcc agcgcctggc tgaactgggt gatcaccgaa
     3541 cccgcctctg ccgcactggc accggaaatt ttcagccctg tcgaaatggc ctccgtcacc
     3601 ttcagcacat catcagcact gtaaccatat tcacgcatcg aggcagccga acgggcaaac
     3661 agggccgcat tatccgaaaa tgcggtgcct gtccgctggc tgatgtccat cagcactttc
     3721 tgtgatgacg caaattcatc cgatgactgc gacgcctgtt tcagacgggc attcacggag
     3781 ctccattcat ccgccagcga aatcaggtgt ccggtggcaa aggcaccggc aaacgcaccg
     3841 gtcattccga cagccgaagc gcggatttcc gtcaactggc tgttcagctc agccagagcc
     3901 cggcgctgct ccctggctgc cgcagcagcc tgacgtccgc cattctgcag ggtccggtaa
     3961 tattcactgc ccatacggga cgcccgctgg atctccgact ggaatgactg tgaatttgcc
     4021 gaaattttga taatcagttc acgtaacgtc gccattcacc tttctccggg cgaaaaaaaa
     4081 cctgccgcag caggttttat tcatcagcag attattgatt aagttttggg taccagcccg
     4141 ggattcagac tttccggact cagtgtatac ataccgccat tgaatgggtc cacaagcaac
     4201 cagccaacaa taccaccaaa gacaaagtta ccaccaatgt accatccatt agccgatgcc
     4261 ttgatcggca gcgttacggg ctgataattc tctttactga atgtgatctg ataactcttt
     4321 ttcccgaagt aactgccgtc agatttttca agtgttacat tctgcggtgt tttgcccgaa
     4381 gaaattgttt tccctgactc atctttaata ataaaatccg caccagacgg attactgttt
     4441 acctgtacag tctgcgtttt atctccgaca atggttgcac accccgtcag aaaaaagctg
     4501 acagagaaaa tcaacagcat gaatttcttc ataaaatggc tccgttcagt taattttccc
     4561 cgaaaatact gtaataaaga tctgcaaagt taaaattaat tttccgaagc aaataacaga
     4621 gcatcctcca gagcggccaa aggatctgcc tccggcttat cctcatcctc gccccagcag
     4681 agcatggcgt ccttcagtgc aacattcatc ccctgtgccc cgaaaaccgc tttcacgatc
     4741 tgtgcattac ggatatcccc gcgctcatca cccagcgggg ataccctgtc gaactccatc
     4801 cacatcatcg cctcgctcgc actcaggctg tggcgcagtt cggataaggt gcgccccaga
     4861 cggagcgcaa gtcgcatcag aaagcgaatt tccgggcggg ctactttttt ctggccgact
     4921 ctgcatcagc gatcagttcc agtgcctgac gcagcaagcg ggcatgtacc ggaccataga
     4981 cggccagcac ctgctcacgg tcgtccggag tgaacacccg ctgcaggtcc gtatcacaca
     5041 ggacatcgca gaacagcgtc acatccgctt ccaggttacg gcgggttttc gccaccaccg
     5101 acagggtatc gtcatcctct ccatcaccat tgagcacgtc ctgccacaga taccaggcct
     5161 ctgccgaagg ctcccgcagc accacgctga cattacccca ttccggcacc ttcaccgttt
     5221 tatgacgaaa ccctgacagt ctggccagcg ccagcgtttt cagctccttt ttcatgatga
     5281 cccatcccct tatccggcgg ctgcgctcac tgtcactgta cattcaacag acgtcacact
     5341 ctgtgctttc tctgccgaat cagtcaccac gcaggtatat ttccccgcat cagcggactg
     5401 cgcacctggc ttactgaagg tgtctgtcgt ctgcccgtca accggctgac catccttctt
     5461 ccaggcgtat ttatacggcg gcgttccccc gttggcactg actgacattg tcagcagcgc
     5521 accggtattc acggtaagtg tcttatccag atttttcaca aacgccagcg gtaccacaaa
     5581 ggacaccggt ttgcctttca gacgcagtga gaacgttgca gccaccacgc cgttggtacc
     5641 ggatgaccag gtgtgctggc gcacttccgc caggaattta aagcccttac cggacggaaa
     5701 ctgcacctta aacgcataca acgcgtcatt gtcataagca tcacgcaggg cgttctgggc
     5761 ctgattcaga taaaaattac ccgacatgga aatctcggac gacgccccca gaccattgat
     5821 attctcctgc tctgtggagc agagcgtggt cacatcaata tcctgtttct gaccggcggt
     5881 gaactggact tccttgatgg tgcagtccag gcgcagatat tccgccttat ccatagtttc
     5941 agcagtcgcc ggggcagatg aaatcatcac ctgcgtcagc tgtgagcgtt catacaaagc
     6001 agacattctg cctcctgata ataaaaaacc cgcacgcggc ggggtatggg ttttgtagaa
     6061 aaaagaaaaa gtcacaccgt gacctgaaac tccagggttg cacggtaaca gcggttttcc
     6121 ggaatatagt cctgcatttc actgacggat cccggggcca gcagcattat ggcttcacgg
     6181 gcgtcctgac gtatctgacg cgcctgcgtc acagtcccgg cataaacgtc tatctgcacc
     6241 gccactgagg actccgcctg cccgcccatc acgtccgcag acaccgatga aatcaggctg
     6301 aaaaccaccc acggaagcgc caccgacggc ctgccatcca gcagggggac cacatacggg
     6361 tacacctgcc cgccggcaag atgcgccaga tgaggataca aatccgcctc cgtcatcgtc
     6421 tcagtacctc atcaatggcc cggttcatcc gcgcaatcgc cacctgcgct gcctgttcac
     6481 tgcgcacatc aaacgccggg cgcacaaacg ggtgcggtgg catattcacg gtccccattt
     6541 ccacaaaccg ccagtagaaa gcattgcgcg ggttatccgc cttcatggtg ttatcgctgt
     6601 taccgg
//
LOCUS       DAZGHF010000202         6604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGHF010000202 DAZGHF010000000
VERSION     DAZGHF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.203"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..172
                     /locus_tag="ACHK3A_004629"
     CDS             <1..172
                     /locus_tag="ACHK3A_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742609.1"
                     /translation="SYLSFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIEDE
                     IIVCATYLIPDI"
     gene            174..518
                     /gene="ymgG"
                     /locus_tag="ACHK3A_004630"
     CDS             174..518
                     /gene="ymgG"
                     /locus_tag="ACHK3A_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO8742610.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            522..857
                     /gene="ymgD"
                     /locus_tag="ACHK3A_004631"
     CDS             522..857
                     /gene="ymgD"
                     /locus_tag="ACHK3A_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO8742611.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(915..3557)
                     /locus_tag="ACHK3A_004632"
                     /pseudo
     CDS             complement(915..3557)
                     /locus_tag="ACHK3A_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
     gene            complement(3955..4173)
                     /gene="ymgF"
                     /locus_tag="ACHK3A_004633"
     CDS             complement(3955..4173)
                     /gene="ymgF"
                     /locus_tag="ACHK3A_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO8742612.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLINRLRERLVSHRDQH"
     gene            complement(4305..5828)
                     /gene="pdeG"
                     /locus_tag="ACHK3A_004634"
     CDS             complement(4305..5828)
                     /gene="pdeG"
                     /locus_tag="ACHK3A_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8742613.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIREALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTRSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(6164..6412)
                     /gene="ymgC"
                     /locus_tag="ACHK3A_004635"
     CDS             complement(6164..6412)
                     /gene="ymgC"
                     /locus_tag="ACHK3A_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO8742614.1"
                     /translation="MNNSIPERFILQCALFKNLEREVFMTHGYVDSYIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVIS"
     gene            complement(6525..>6604)
                     /gene="ariR"
                     /locus_tag="ACHK3A_004636"
                     /gene_synonym="ymgB"
     CDS             complement(6525..>6604)
                     /gene="ariR"
                     /locus_tag="ACHK3A_004636"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO8742615.1"
                     /translation="TSDILKADVIRKTLEIVLRYTADDM"
BASE COUNT         1888 a         1508 c         1376 g         1832 t
ORIGIN      
        1 gagttattta tcatttgaga ttattcttat tcccgtaaaa aacataattc ctatcatcac
       61 agtcacgctc attttgaatt atctgaataa tagcgagcgt tctttagtta agcagatctt
      121 aattgaagac gaaattattg tgtgtgcaac ttacttaata ccggacatct aagttgaaga
      181 aaaaaatact tgccttcggg cttatctcag cattgttctg ctccacgcca gcgatggcgg
      241 atatgaaccg caccacgaaa ggtgcattgc taggcgcagg cgttggttta cttactggca
      301 atggtgttaa tggcgtactg aaaggtgcag ctgttggcgc tggtgttggt gcagtaacag
      361 aaaaaggccg cgacggtaaa aatgcgcgta aaggtgcaaa agtgggtgcc gccgttggtg
      421 ctgtcactgg cgttctgacc ggcaatggcc tcgaaggggc gattaaaggt gccgtgattg
      481 gcggtacggg tggtgcaatt ctgggtaaaa tgaaataagg aatgatgatg aaaaaatttg
      541 ctttactggc gggtctgttt gttttcgcac caatgacctg ggcgcaggac tacaatatta
      601 aaaacggttt gccgtcagaa acctatatta cttgcgcaga agccaacgaa atggcaaaaa
      661 ctgacagcgc acaggttgca gaaattgttg cggtaatggg taatgccagc gttgccagcc
      721 gtgatttaaa aattgagcaa tctccggaac taagcgccaa agtggtagaa aaactgaatc
      781 aggtttgcgc taaagacccg caaatgctgt tgatcacggc aattgatgac accatgcgtg
      841 ctatcggcaa aaaataatat ttcatatgtt accgcgtcga tgaattcgac gcggtttttt
      901 agctaatggc gggatcagaa cttatatttc aggcccacca tcattgcggt gtcattgtag
      961 ccattatccc ccagctgcac gcccacatta ccccagacgc tggccgccgg attcagattg
     1021 ccatttacgc caagtttaat ttcccccaga ttacgcgccc cgtcttgttc aactttcaca
     1081 ccgttcattg agacagcaaa atctttactg ttatggatcc agttcatttc aacaaaaggt
     1141 tcaaagtcgt caccgtgcgg accgctgttt ttctcctgag tacgaataaa ggtgcggaag
     1201 ccgagtcggg tctggatgtt gttatttgcg tcattctcaa tacgtgagcc attggcttcc
     1261 gtgtgcgcat tatgatcgac gcccatccag atagcctgtg gctggaagag ccacgtatat
     1321 ttcgccgctt ccagaccaaa ggatttattc agggcaaaac cgtaacccgc ttccagcgag
     1381 acggttgcac ctttcgcgct ataggattca taggctaatt catccccttt caccgagttt
     1441 ttaaaccagc tatattacgc ccaggtgtca atgtatgcgc cttttttact gatgtcatcg
     1501 gcaaaccagg tggcatacag ccctgcgcta tagcctctga cactcccttt cgaacgataa
     1561 tccgacacgc tggaatgagt tgagttgtag tcgcgggcat aaccagccaa cactcccaaa
     1621 cgccaactgt cactgtcggt aaaaccaccg gttaacagat ccgcccctaa ctgcgagacg
     1681 tagcgatgcg aggtcgttct caactgtccg ttgctgtcac gccaggcatt atgcccgcca
     1741 atttgacgta gccaaagccg gcttgaacgc tcctgttaag ttacaggatc gatgtaacgc
     1801 gtttcacctg cacgatcgtt cagacgcata acaaaaagag tgttagcggc tgccaggttg
     1861 gcgacgtaac ttccagcttc agggcgcagg acgggggtag gcgcaacagt aggcgctgaa
     1921 cttcctccgg agtttgtttc accgccgctc gttgcctgga agttagtcag ataccagttt
     1981 ttattggtcg cttcggtatt acctcgggtt aatgaatatt cgtaggcacc ggcgaaaatc
     2041 ctcgaatcct taataaattc tccatttgat tccccctcaa cgctgataat ctctatccca
     2101 tttaacgtgt aagccccgct gccgccagca ttggtaactt taactcgggt tgttccagag
     2161 gtatttccat taataacgac tttatccgta gctgaattat catcacccaa ttcagttctg
     2221 agatgcaata cgccattatt accaacgtaa ttttcagtta ttgtcaatct tgttggttcg
     2281 aaatcctttc catctgcccg ggaaatatag accgtactat tatcaaccgt gagtttgctc
     2341 accgttgagt cagctttaat atcccaggta ctattatctg aaagcgacag ataggtatga
     2401 gtgttatcat ctgttgaaag attggctgaa cccgttattt ttgaattagt tgcatcgacg
     2461 gtcaaatgac ctttaatatt attaataaaa acatctgcag ccgatgatac ctcactatcc
     2521 gtaatggtca cgttactcgt ctcattttta tggtagagga tttcatttgc tgaccgtagt
     2581 gagctaccag ataaaatatc taccgttgct tcctgaccat ttatggaaga ctccagcaca
     2641 attccagccc cctgatgcgc gactgcagat gtatttttca gcgtcacctt gccgccatta
     2701 taaattttag caatcgcggc gtcaggaccc gttgtataaa tttctccctg gttaaatgaa
     2761 actgtcccct ggcgagcagc gatggcatga gcattgtccc catgcgtggt gatatcaaca
     2821 tcggttaatg taacgctggc attggtataa tcaagcttgc taccctcctt gacgacagtg
     2881 ccattagcgt aagcggcata actttctgtt ccgtgggttg ttatctcccc ttttgccata
     2941 tccagttttg ctttggcgtt attttcatta cttcctgtat cggcagtact ttcttcatca
     3001 agagaagata cacacgcacg cgcgcttccg ggagatgcac acgcaagaac gccgactgcg
     3061 tgattacccg cagtaacgat aaaagtatta cctccgtcag aaaaagaggg ggtagccgaa
     3121 ccgtcagggt tgactcttat tatggtatca gtattcaggt ttccatcaac ttgacttaac
     3181 aaaccaacag aataatctcc cgtcgtgaca atcgtattat taccccgcaa agtgacagca
     3241 ccgccatatt cacttacgac tgcgctagct gtatatgctg tgcttagaga actattgtca
     3301 gaaggatata aaccgcccgt tgttacaatg acgccattgt taatggttaa attgctaccc
     3361 tttcctgcaa cagatacacc gtaacttcca ttacccgttg ttataactag cgcattatca
     3421 atatctttta cttctccatc ttttatttca acaacatgtt taggttcatc gtgctgaata
     3481 aactgttcag catatgcatt acccaccgcc aaagcaataa gtcctaaact aaacccgggg
     3541 agttttttca gtttcataga ttaaccatcc attcattgga ttaacaaaca ttcctggtca
     3601 aataacctat gagttcagat taccaaaacg gagggtaaag acaaaaacaa aagcacaaaa
     3661 aatttaaata gataaacagc gacaatatat aaatgataaa taaggcggca ttatcatttt
     3721 tcaccttatt ttcatgacat tgatcacttt ggggtgacga ttttgtttaa caaataactc
     3781 ttcgacatca aaaccattaa tagttgccat agaaattatt tcaatgatat ataaaacaac
     3841 tgtgataaca ccagagttga caactatttg cgcaataatt cctttcttca atgatccagg
     3901 cgttcactgc cagcaaccca ttcagccgca tactgacagt gagaaactga aagattagtg
     3961 ctgatccctg tggcttacga gtctttccct taatcgatta atcagattga tcgccagcca
     4021 aacaagggca ttaagcaaat ttatcgtcac aagcacttct cccagcccgg tactgggttt
     4081 tgcaataagg atgaccagga gagtgatcag cataaatgca atggaaaaga tgatatagag
     4141 agtgaaataa tccagattat tactattgtt catgctacta atggcaatcc tgtcaaaact
     4201 ttcaaacaat tatagcaaac aaaataatga acaatagtaa caagaggcaa cgagctaatt
     4261 agctgtctat aattacaccg cactatggcc ttactgatcg attttcactc aaccacaacc
     4321 ttcaccttcg gtttagaaag gataattttg accaggtcga tgtatgtaac aggtttatag
     4381 aaataataac cctgctgaaa tgtgatatta ttttgattca gatagtcaag ttgttctttc
     4441 gtttcgacac cttcagcgac gatactgatc gaaagtttac gcgccagatc caatacacaa
     4501 tccagaattc gggttgattc tgggtctgcg ttgacgcggc caacgaaact gtgatcaatt
     4561 ttgatataat caatatgcag gtcatgcaga taagaaagcc ctgagtagcc agtaccgaaa
     4621 tcatccagcg cgatgacaaa accattttca tgcagaatgt tcaaccgctg aaccagactt
     4681 tcatcaacat ttaatggctc acgctcggta acttccagaa cgaggtttaa atcgcggcgg
     4741 gtaaaactgt cacggtaatt taagcactcg tcgacaaacg tcggcgaaat aatatgcgag
     4801 gcgctaaaat taattccaat atgaaaacct tccggcaatt tactcgcgat agcgttcatc
     4861 tgtctggcaa cctgattcat caggcttcgc gtaagcggaa cgattaatcc ggatttttca
     4921 gcaagtggaa taaatgccgc aggtgatata tatccaccgt gaggttgttt ccagcgggct
     4981 aacacctcaa ctccccgcaa tgtcccttcc cgaccattta ccacaggttg gtaaaaaggg
     5041 acgatctccc cattatttat agcccgtcgc aggatctctt caggcgttgc gcttttattg
     5101 aaatacctat caagcaggaa ggcagcggcg caggcgatta acagaataaa tatcaaaatc
     5161 ccaaagcctt tatcgataag tctgtagaag ctaaagagtg gcggcgggtt aaagataata
     5221 gtgaaatgat attttgtaga gtgaaccttc tcggtatagg gatagtgtcc attaagcgtc
     5281 atcacatcac ccgtaggtcc aataatgtca tctgccacgc gtaatacata ccttacccct
     5341 ttcaagggta cattaagcgc ctcgcgaata tgctgatcac ttatcgtaac caaaatgcgc
     5401 gtatctgcaa attggctctg atagagcagg ataggtaatc tattaacggt gtcgattgcc
     5461 ggagccagca gtaaattact gtccgggaaa acaggaatac gagacagtag gacccgattc
     5521 cccggtaagg atgtacacca gaccgttccc tgtttgagaa tgatgattgt gcgcaggtga
     5581 ggcttcagag ctgcttcagt gccaagctga tattgctcct cgaggtcgca ttccttcccg
     5641 gcaatgttaa ccgccgtttg agtcgcctgt gacgcttctt caagtatatg attgatattg
     5701 ttggcggcat atctcgctcc cgccaggtac tctgctttcg cggaatacca gagctggatg
     5761 ttcaaaatag cgacaccggt tataaacaag catatcgcaa cgaggatggg tatgagtgta
     5821 ttgcgcattc gcttgtcccg gacaaagggt ttaatacatg aaaatgacgt tcattcgctc
     5881 gcgctggcgt ctacagagag catggtagag agcatcacaa atatccacgc cgaagataaa
     5941 attttctcca acttcatcta acctattaat tattgagcac aatgtaggca gaattaaaca
     6001 aattaggtgg gatatcaggc gtcaagaatg aaaaacgagc gctctccatt cctgtttgcc
     6061 ccctgatatt gcggatatat taagaaagca taacgcctga aatgctcact ttgcaccagc
     6121 atggtgatac agctgatgtt tattctataa ccttactcaa gttctaagag atcacggatt
     6181 ccctgtcatt aataatgata tccgttagtg tagtcttatg catttcaatg tactgcaata
     6241 tttgcagata gagatcagaa agaatcacac tggtttcatc ttttaaacgc aagcgtaaag
     6301 cctgatcaat aatataacta tcaacatacc catgcgtcat gaacacctcc ctctcgagat
     6361 ttttaaacaa agcacattgc aaaataaatc tctctgggat tgaattattc atgtcagcct
     6421 cgtagaaata ttaacccaat aaaattatgc tgtgtatagt ctaaagcgac agacaaaaat
     6481 aagatgaatg agttttctat aaacttatac ttaataatta aaagtcacat atcatccgct
     6541 gtgtatcgca acacgatttc cagtgttttt ctaatcacat cagctttgag aatatcactt
     6601 gttg
//
LOCUS       DAZGHF010000203         6532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGHF010000203 DAZGHF010000000
VERSION     DAZGHF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.204"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..280
                     /locus_tag="ACHK3A_004637"
     CDS             <1..280
                     /locus_tag="ACHK3A_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742616.1"
                     /translation="EFNTLKNRKEFLSSVINVNETRLQASAPAMPAIEALNAFIDKGD
                     YLKSLYVNKTRTAAEQKRLNEILRAQVISGLKASEGLAKLLIASGIML"
     gene            291..599
                     /locus_tag="ACHK3A_004638"
     CDS             291..599
                     /locus_tag="ACHK3A_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742617.1"
                     /translation="MNDVQNRRILALLDMLYNTLGNKTTCNVCHKYFILILTVMNNLT
                     INYGTETSPELSGISYEDANALITELILFIEDVDAENHEAYQGHLNEITRILNFAIAA
                     "
     gene            complement(651..1241)
                     /gene="cobO"
                     /locus_tag="ACHK3A_004639"
     CDS             complement(651..1241)
                     /gene="cobO"
                     /locus_tag="ACHK3A_004639"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO8742618.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(1238..1996)
                     /locus_tag="ACHK3A_004640"
     CDS             complement(1238..1996)
                     /locus_tag="ACHK3A_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO8742619.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2216..3265
                     /gene="sohB"
                     /locus_tag="ACHK3A_004641"
     CDS             2216..3265
                     /gene="sohB"
                     /locus_tag="ACHK3A_004641"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO8742620.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3301..3552)
                     /locus_tag="ACHK3A_004642"
     CDS             complement(3301..3552)
                     /locus_tag="ACHK3A_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO8742621.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3932..6529
                     /gene="topA"
                     /locus_tag="ACHK3A_004643"
     CDS             3932..6529
                     /gene="topA"
                     /locus_tag="ACHK3A_004643"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO8742622.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1717 a         1528 c         1719 g         1568 t
ORIGIN      
        1 agagtttaac actctgaaaa acaggaagga gtttctaagt tcagtaatca atgtgaatga
       61 aaccagacta caggcatcgg cacctgccat gcctgcgatt gaggctttga atgcctttat
      121 agataagggt gattatctga aaagcttata cgttaataaa acacgcacgg cagctgaaca
      181 aaaaagatta aatgaaatat tacgcgcaca ggtcattagt gggttaaagg cgtctgaggg
      241 gctggcaaaa ttacttattg ccagcggaat aatgttatag gaggcttttt atgaacgatg
      301 tgcaaaaccg tagaatactg gcattacttg atatgctgta taatactttg ggtaataaaa
      361 ccacatgtaa tgtttgtcat aaatatttta tccttatcct cacggtgatg aacaatctga
      421 cgattaacta cggtactgaa acttcgccag agttaagcgg aatcagttat gaagatgcca
      481 atgcgttaat tactgaactg atattattta ttgaagatgt tgacgctgag aatcatgaag
      541 catatcaggg acatttgaat gagattacac gtattctgaa tttcgctatt gccgcttgaa
      601 tataatcatg attaaagcac ctcaagaggc ttcttttgag gtgctttcac ttagtagtca
      661 atccctatct gcgcttttac accggcatca aacgcatgtt tcacggggcg taattcactc
      721 accgtatccg ccagttcaag aatatcccga tgacagcctc gacccgtgat aatcaccgtc
      781 tgttgatgtg gacgttcatt taacgcctgc accacttctt ccagtggcaa atagtcatac
      841 gccaccatat acgtcagttc atcgagcaaa accatatcca gtgaaggatc ggccagcatt
      901 cgtttggcat gttgccagac ttcacggcag gccgcggtat cagattcgcg attttgtgta
      961 tcccaggtaa agcccgttgc catcacctga aactcaacgc catgtggctc cagtagattg
     1021 cgctcgccat taggccaggt gcctttaata aactgcacga tacccacttt tttgccatgg
     1081 cctactgcac gagttgccgt accaaatgcc gcggtggttt tgccttttcc attgccggta
     1141 aagacgatga taataccgcg ctcatcctgg gcctgggcca cacgagcatc gactttttct
     1201 ttcactcgct gctgacgctg ttggtagcgt tcatcactca ttgggaaatt cctggtttac
     1261 ggcccggttg ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc
     1321 agaggtagag cggcatgata tcagcgggtg ttttaagttt ctgtggatct tcggtcggga
     1381 aggcgctggc acgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg acgcgcaggc
     1441 gctgctgata ttcatcggcc agtacctgca tcatcccttc ggtggcaaat ttcgacgctg
     1501 catatgcgcc ccagttggct cgtccctgac gtccaacgct tgatgaagta aagaccagtg
     1561 aaccggcgtc cgatttgagt aataaaggaa gcagtgcctg ggtgagcata aaggtggcat
     1621 taacgttgac ctgcatgacg tcctgccaga cctgcggatt ttgttcgctc attgggcaaa
     1681 catcgccgag caatccggca ttatgcaaaa caccatccag acgcggataa ttaacggcaa
     1741 tgcgctgtgc cagttgttgg caatcttcgg acgtgcaggt cagcaaatcg agaataaacc
     1801 actgtggctg acgtccagtt tcttcgttta tgtggctggc tacctgacgt aatttttctt
     1861 cattacggcc caacagaatc actgtcgcac catagcgtgc atacgtcatc gcggcttcac
     1921 ggccaatacc atcgctggct cccgtcacca ggataatgcg atcattgagt aaatcttgtt
     1981 ttggctggta atgcatggct actcctcaac gacgttgtct gtcgcgctgc cagtgtactt
     2041 tatgactttg gggctttatg cctgaaacag aagggcattt caatcagcct ggggcgataa
     2101 caccgcaaaa tggatacttt gtcatacttt cgctgcaata acatctctgc gagacggctt
     2161 aacatgcctg ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga
     2221 attgttgtct gaatatggtt tgtttttggc gaaaatcgtt accgttgtgc tagcgattgc
     2281 ggcgattgcc gccattattg tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt
     2341 acgggtcaac aatctcagcg aacagtataa ggagatgaaa gaagaactgg ccgcggcgct
     2401 gatggactca catcagcaaa aacagtggca caaagcgcag aagaaaaagc acaagcaaga
     2461 agcgaaagca gcaaaagcga aagccaaact gggcgaggtg gcaactgaca gtaaacctcg
     2521 cgtctgggtg ctggatttta aaggcagcat ggacgcccat gaagtgaact cgctacgtga
     2581 agagataacg gcggtactcg cagcattcaa accgcaggat caggttgtgc tccgtctgga
     2641 aagccctggt ggcatggtgc atggttacgg gttggcggct tcgcagctgc agcgtctgcg
     2701 tgataaaaac attcctttaa ctgtaacggt agacaaagtc gctgccagcg gcggttacat
     2761 gatggcctgt gtggcggaca aaattgtttc cgcaccgttt gctattgtgg gttccattgg
     2821 cgtggtggcg caaatgccca actttaaccg cttcctgaaa agcaaagata ttgatatcga
     2881 actgcacacc gccgggcagt ataagcgcac gctgaccttg ctgggtgaaa acaccgaaga
     2941 agggcgggag aaattccgcg aagagctgaa cgaaacgcat cagttattta aagattttgt
     3001 gaagcgtatg cgtccgtctc tggatattga acaggtggca acgggtgaac actggtacgg
     3061 acaacaggcg gtagagaaag gcctggttga tgaaatcaac accagtgatg aagttattct
     3121 tagcctgatg gaaggccgtg aagtggtaaa tgtacgctat atgcagcgta aacgactcat
     3181 tgaccgattc accggcagcg cggcagagag cgccgatcga ttgttgctac gctggtggca
     3241 gcggggtcaa aagccattga tgtaaaagac aaacgcgagg gtaagacctc gcgttttgct
     3301 ttaatcaacc aggtgatatt tttctgaaag cacatgggcc aggtgtttga acatattaaa
     3361 caccgcggtg cttttggctg ttggcaatcc ttgttcatct aaaaagtagt cgccggtaaa
     3421 taccagcacg ccattacgct gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc
     3481 atgctcacga atgattttgt tggcctcttt caacagcgtc tcgcggtcga tgggttgtgt
     3541 ctctttgttc attatttact ccttaaacaa ggacattagt ctacgccagg catggcttgc
     3601 agacaaatat accacgctgg tggcaagagc gccttactgg caactttgga ttttgcatgc
     3661 taataaagtt gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc aatctggcaa
     3721 tagatttgct tgacattcga ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc
     3781 tgttaactca gtcacctgaa ttttcgtgaa cagagtcacg acaaggggtt gatatccgca
     3841 gagagcgagt ccatatcggt aactcgttgc cagtggaagg tttatcaacg tgcgacgcat
     3901 tcctggaaga atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc
     3961 cccggcaaaa gccaaaacga tcaacaagta tctgggtagt gactacgtgg tgaaatccag
     4021 cgtcggtcac atccgcgatt tgccgaccag tggctcagct gccaaaaaga gtgccgactc
     4081 tacctccacc aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg
     4141 tatgggggtt gacccgtggc acaattggga ggcgcactat gaagtgttgc ctggtaaaga
     4201 gaaggtcgtc tctgaactga aacaactggc tgaaaaagcc gaccacatct atctcgcaac
     4261 cgaccttgac cgcgaagggg aagccattgc atggcacctg cgggaagtga ttggtggtga
     4321 tgatgcgcgc tatagccgag tggtgtttaa cgaaattact aaaaacgcga tccgccaggc
     4381 atttaacaaa ccgggtgagc tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt
     4441 tatggaccgc gtggtggggt atatggtttc gccgcttcta tggaaaaaga tcgctcgtgg
     4501 tctgtctgcc ggtcgtgtgc agtcggtggc ggttcgcctg gtggtcgagc gtgagcgtga
     4561 aattaaagcg ttcgtaccgg aagagttctg ggaagtcgat gccagcacga ccacgccatc
     4621 tggtgaagcg ttggcgttgc aggtgactca tcagaacgac aaaccgttcc gtccggtcaa
     4681 caaagaacaa actcaggctg cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga
     4741 acgtgaagac aaaccgacaa ccagtaaacc tggcgctcct tttattacct ctacgctgca
     4801 acaagctgcc agcacccgtc ttggatttgg cgtgaaaaaa accatgatga tggcgcagcg
     4861 tttgtatgaa gcaggctata tcacttacat gcgtaccgac tccactaacc tgagtcagga
     4921 cgcggtaaat atggttcgcg gttatatcag cgataatttt ggtaagaaat atctgccgga
     4981 aagtccgaat cagtacgcca gcaaagaaaa ctcacaggaa gcgcacgaag cgattcgtcc
     5041 ttctgacgtc aatgtgatgg cggaatcgtt gaaggatatg gaagcagatg cgcaaaaatt
     5101 gtaccagtta atctggcgtc agttcgttgc ctgccagatg accccagcga aatatgactc
     5161 cacgacgctg accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg
     5221 ctttgatggc tggacaaaag tgatgcctgc actgcgtaaa ggcgatgaag atcgtatctt
     5281 accggcagtc gataaaggcg atgctctgac gctcgttgaa ctgacaccag cccagcactt
     5341 taccaaaccg ccagcccgtt tcagtgaagc atcgctggtt aaagaactgg aaaaacgcgg
     5401 tatcggtcgt ccgtctacct atgcgtcgat catttcgacc attcaggatc gtggctatgt
     5461 gcgtgtagaa aaccgtcgtt tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct
     5521 tgaagaaaat ttccgcgagt taatgaacta cgactttacc gcgcagatgg aaaacagcct
     5581 tgaccaggtg gcaaatcacg aagcagagtg gaaagctgta ctggatcact tcttctcgga
     5641 tttcacccag cagttagata aagctgaaaa agatccggaa gaggggggga tgcgcccgaa
     5701 ccagatggtt ctgaccagca tcgactgccc gacctgtggt cgcaaaatgg ggattcgcac
     5761 agcgagcacc ggggtattcc ttggctgttc cggctacgct ctgccaccga aagagcgttg
     5821 caaaaccacc atcaacctgg tgccggaaaa cgaagtgctg aacgtgctgg aaggcgaaga
     5881 tgctgaaacc aacgcgctgc gcgcaaaacg tcgttgcccg aaatgcggca cggcgatgga
     5941 cagctatctc atcgatccga aacgtaagtt gcatgtctgt ggtaataacc caacctgcga
     6001 cggttacgag atcgaagagg gcgaattccg cattaaaggt tatgacggcc cgatcgttga
     6061 gtgtgaaaaa tgtggctctg aaatgcacct gaaaatgggg cgtttcggta aatacatggc
     6121 ctgcactaac gaagagtgta aaaacacgcg taagatttta cgtaacggcg aagtggcacc
     6181 accgaaagaa gatccggtgc cattaccgga gctgccgtgc gaaaaatcag atgcttattt
     6241 cgtgctgcgt gacggtgctg ccggtgtgtt cctggcggcc aataccttcc cgaaatcgcg
     6301 tgaaacacgt gcgccactgg tggaagagct ttatcgcttc cgcgaccgtc tgccggaaaa
     6361 actgcgttat ctggccgatg cgccacagca ggatccggaa ggtaataaga ccatggttcg
     6421 ctttagccgt aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa aggcgactgg
     6481 ctggtcagca ttttatgttg atggcaaatg ggttgaaggg aaaaaataac ct
//
LOCUS       DAZGHF010000204         6525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGHF010000204 DAZGHF010000000
VERSION     DAZGHF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.205"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..3649)
                     /locus_tag="ACHK3A_004644"
     CDS             complement(<1..3649)
                     /locus_tag="ACHK3A_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO8742623.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PLTEAIPSPPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGIMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLYFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTNDDRLAHSPGDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVNVTGADGYYNATLDYSDGCTTVTDGKGIHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAVHGQLSRYTAADGAVWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAGTGAEGFRYDRHGNLLAWTDGNGVDWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYLLDNCGRVTEEHDWGGVTWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEIITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEEATWGGLVPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQ
                     SAGRHRISEETE"
     gene            complement(3669..4130)
                     /locus_tag="ACHK3A_004645"
     CDS             complement(3669..4130)
                     /locus_tag="ACHK3A_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO8742624.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRIDQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(4158..6059)
                     /locus_tag="ACHK3A_004646"
     CDS             complement(4158..6059)
                     /locus_tag="ACHK3A_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO8742625.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVRPPLWRA
                     GLRQNFRIFQNEDIRSILGTILQENDVTEWSPLFSEPHPSREFCVQYGETDYDFLCRI
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDWPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTEHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPKPKPSVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPANQDSSCWIRVAQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HEDNRSPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGKEQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVMIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKQIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1239 a         1944 c         1775 g         1567 t
ORIGIN      
        1 gctcagtctc ttcacttatc cggtgcctgc ccgcagactg cgccttcagg tagccgcagc
       61 tgtcatagcc ataagtctca tcgccttcgc catagcccag cccggtgcct gaggctgtca
      121 ccgccgtgac ctggccgttc cggtccagcc ggtactcccg gcgtgtttcc cgtccgtaat
      181 cttcccggct ggtgacacct gacacgttac ccgcgctgtc gtaacggtac tcccgtacca
      241 gtgacggaac cagcccgccc catgtggctt cttccggaaa aaatccggcg cgctggctga
      301 caggctggcc ggctgcattc cagccctgct cacaggcaaa accccggtta ctgtgccgga
      361 gcacttcacg tcctgcctca tcacggctga acgtcagcgt ctcatccccc ggcaggataa
      421 cctccgtcag ttctccggcg gcgttgtagc ggtaggtggt tttcagcagg gattcatcgg
      481 ggtgtctgtc gcgttcatct tccggtggca ggatacagcg gatgacggtg tgttcatcgg
      541 ggtaatggta ctcaatggcc cgtcggccct gagtggtgac attcacccgc ccgcgttcgt
      601 catagccggt gcgctgtgat gtgccgtccg ggctgaagat acccgtcagc ctgccggatt
      661 tgtcatacgc atactgcgtt ttgccttccg gcgtgatgat ttctgccagg cggcctgcgg
      721 cgtcccggct gtagaggatg gtttcctcca ggccgttaac cctggcggta cacaggccat
      781 cggcgtcata gcggtaacgc caggtcacgc cgccccagtc atgctcttcc gtcacccgcc
      841 cacagttgtc gagaagataa cggtatgact caccctgcgg gttgatgact tctgtcagtt
      901 gcagcgtgtc tttatcatag cggtactgcc agcggtggcc ttcaccgtcc gttcgcgcca
      961 ccggcaaatc aaacgggccg tattccattg tccagtccac accgttaccg tccgtccagg
     1021 ccagcaggtt gccgtggcgg tcatagcgga atccttctgc gccggttcct gcccgctcaa
     1081 ggccgctcag tttatggtgc tgctgccact gcaaccgcac tgactgaccg ccggggccgc
     1141 gcatcttcac cggcaggtcg tgaccactgt attcggtgtg gtgttcacgg cccatcacgt
     1201 cggtgagtgt ttccggccgg ttgtggtggt cgtactctat gcccagacgc cgttcatcct
     1261 ggcggaatat ccccgacagc agcccctgag cggtgtacgc cagccgggtc tcttcgccct
     1321 gtggcgatac cagactcacc ggcaggccgc gttcatcgca ctgctgtgtc cacgtctgcc
     1381 cggcagggtc ggtgatgttg ctgagcaggc cgcgctcatc atagcggtac aaccacaccg
     1441 cgccgtctgc cgccgtataa cggcttaact gaccgtgtac ggcgttgtat tcaaatttct
     1501 ccacccgtcc ggcaggggag tggcgggcca gcaggtgatg gaattcatcc cattcatacg
     1561 tggtggtgct gccgtccggc gcttcttccc gcagaatatt gccgtcagga tcatagtaat
     1621 aacggtgaat gcccttgccg tccgtcacgg tggtgcagcc gtcactatag tccagcgtgg
     1681 cgttgtaata gccgtccgcc ccggtgacat tcacgcaccg gccctgttca tcgtaggtga
     1741 aacggctcca cgtctggtcg ttatcggacc agcggatgat ccggtccgca gcgtcgtact
     1801 cataaaacag gtggtagtcc agccgcgcat ccgcttcggt cagccgcccc cgcgcatcct
     1861 gttcgtaggt cgccagcgtc tgtatgccgt tatccgtgcg gtgaatggct ttcaggtagc
     1921 cgctggcgtg gtacgtcagt ttaagccgga tcccgtcggt gtgactgact cccgtgatgc
     1981 ggtactggct gtcgcgctca aacgtgatgc ggttgctggc ggtgtcgctc atcccgccca
     2041 gcatgtacat ccggtcaccg gggctgtgtg ccaggcggtc atcattggta tcgtcagtca
     2101 ccgggtggcg caggcgcatt cccggaaagg cgtcataaaa gaagcggctg ctcagggcat
     2161 cacggtcgaa cagctccagg taatcgtcgc ggcggcgcag gatatactgg gcgtgatacg
     2221 ggttaaccgc cgtatcactg tctccgtcga aggcaaagta cagcgtggct ccctggctga
     2281 tgatatccac ccggtttccc tgttcacgca cccaggcgta ttcgctccag gagtcgctcc
     2341 agcccacgcc cgtcaggcca tgcagtggca gtacagaact gtggaaacgg gtgaaggaga
     2401 gggggagtgt ctggcccagc gtaaagtcgg tacgttcgtc gcagtatgcg ccggttaccg
     2461 gatcgacagg atctgttacc catctgatga ctttattggc aatacttttt aacgacttac
     2521 ctgttttccc cagcaacgcg ccgagctttg cccccagcag cttcccgctt ttccccagca
     2581 ggtttcccag tttgccgagt ttcgccagat tccccagttt tcccagcagt cccgcgggcg
     2641 gcagaagata gctggctatc gttattcccc ccaggatcat tctctgccac ggcgggaact
     2701 ccggctggat atccagatag gtcacctgtt caccgccaat aaagacgtcc ggcgaacctt
     2761 ccacgattgc cgcgctgcac tccagtttgt cgcccttgcg cgcggcaggc tgattgttga
     2821 taaagacgtt gctgctgccc tgggctatcc tcggtggttt gttctccttg tcgcagagcg
     2881 ccgtgtcctc accggcttcc gggaaatcca cgtctttttt gatttcgcca cgcccgccca
     2941 ccatggtggc aaactccatt aataccggac cggcgggaaa cagaagcgat gcgccggcgc
     3001 tgacgcgatc cataataccg gcattgctgt ccgtcagggt gataacagcg ttcccccagg
     3061 ccttcgccag tggcactgct ttttccgctg ccgattcaag caggtcagtg gcaatatccc
     3121 cccatgaagg ttccccctgt ggtggagagg gtattgcttc agtcagggga ggagggggaa
     3181 gggtgacggc ggccctggct gctggcttac cgttaatttt tacgttttca gaaccggtat
     3241 tgatggcgcc gtccgtgtca caaaaactgt ttatccatga ctcaagctgg ttctgtttct
     3301 cctccacata atcactcgcc ttatagctgg catacatcag cgcaggcacc agaaccagcg
     3361 cataagggcc ggtggcgcag gcggccataa tcaggcaacc ttctgcgata gttactatcg
     3421 cccccagcac ggcccctgcc agtgccccca gaaaactttt atgctgaatt tcatcgccca
     3481 gcctggcggc aggtgggccg gacttctttg ctgccagcat ctgtgcaatg gctttgtcgt
     3541 tggctgcccg tttcatctgg ctggcgacca tcgcctgctg tgacagcatt cgcattgcca
     3601 gcgtaccgcc tgcggtcgct ccctgtgggc cgcctggtcc ttcactcatg tgttatccct
     3661 cgtttatgtc agcgcggctg gtccttttgg atgtcattct ccggtggcaa cggggtgaag
     3721 ctctttatca cctccagcag catcgtcttt tgactgttct tcagggataa ggaggtgctg
     3781 aaagtgaagg tcagcagcgt atctccacgc cgctggaata cggctatctg gtcaatccgt
     3841 ccctggttcc cctcatcttc cggaccgttc caggaataat ccagcagcca ggccggggcc
     3901 tctgccagag tgatttcccg gcaggcatgt tcggtgtacc catcaagttt gccctggtac
     3961 agcgccagcg tttcctggta cagctctggt gtcagggttc cctgtggaac ggcttcgcgg
     4021 gtaatgacca ggttgatgcc actgttatct gtggacacca ggacctgcat tgactgatcc
     4081 tgccattgcg atggcagctg aatatgacct tcattaaaag tgaactgcat aaaatactcc
     4141 ctgataatct gaatgaatca gttgatgaaa acagaaccgc ccacgatggt gatgccatcc
     4201 ttcgacagcc tgattaccgt cgattccccg acctgtagct gaatgccatc cggcgtcagc
     4261 gcaattttgc tttcctccac ggccagcata atttgcttaa ttgccgtata agcctgtata
     4321 ccgtcgcctt cgccaccatt gtttacagcg tcaacatgca ctgaaccggc aatcgtgcct
     4381 ctgtcctgaa aaacatcctc agataaagac ccttttagta cgcctgtgat tttgtggcct
     4441 ttttccaccg taagaaggcg gttaccttcc tggatcatga ccgtctggtc attttttacg
     4501 gtttcgcggt gatcgtgttt tacggtggtg gtacggtcgt tgagtacctc ggtatccatg
     4561 ttcttctggg cgtggatgta gacctgttct ttccctgtcg catcgtcaaa cttcagctcg
     4621 ttaaacccgc tgcccatata tgttttggag cggatggtca tctgcgtctt cgttcccggc
     4681 aggctgcccg gcgagcggtt atcttcgtgg taggtgcgcc ccataatgat cggctggtcc
     4741 ggatcgccgt tgaggaagtc cacaatcacc tcctgaccca cgcgcggtat cgccaggttg
     4801 ccaaatcccg gccccgccca cgcctgcgcc acccgtatcc agcacgaact gtcctgattg
     4861 gccgggttat agcggtccca gttgaatttc acccgcaccc ggccatgctc gtcgcagaag
     4921 atttcctctc ccgcaggccc cgtcacgacg gcgctctgcg gaccgtccac tgacggcttt
     4981 ggcttcggct gaggtcgcca cgtccggtct gccggtatta ccgcaaagtg gttattcagc
     5041 gtggtacctg accccctgcg tcccggcacc gcctgcggct gttcgccgtg cagttcgctc
     5101 gccaccacct gccattcccg gttcaggttc gcctgcggat gctccgtcag cacaattcgc
     5161 cgtccaggcc atatctccgg cgagcggctc attccccgcg cggtttcggc gttgtttcgc
     5221 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cgctcttgaa acgcccgggc
     5281 cagtcataca cttcatactg tgtgcgctgg taatcctgat gctggccttc ctggtcaaaa
     5341 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacaga ggaaggacgg
     5401 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
     5461 gggttccagg ggatttcaaa ggattccggc agatagcgga ccgtgtcgca cagcaccaga
     5521 ctctggtcgg tacttttctg cgcgtgctcc tcatagaaga agatgccttc ctccgctgct
     5581 atccggcaga gaaagtcgta atcggtttca ccgtactgga cacaaaactc acgggaagga
     5641 tgcggctcac tgaacagcgg gctccactcc gtgacgtcat tttcctgcag tatggtgccg
     5701 aggatgcttc tgatgtcctc gttctggaat atacggaagt tctgacgcag tcctgcccgc
     5761 cacagcggcg ggcgcacctt catgctgtac agcatctggt ttttgtcgtt ctccccgagt
     5821 tcaaaccagg tcaccacgcc tttcacccgg cgctgtacgt catcgccctg ccataccgtc
     5881 aggtaggcca ttttgtccag cacctgcgca aattcaaggg agagaaactg ctgactgacc
     5941 agagaaaggt caagggaaaa aagtgaagag agtgactggg tgagatgaaa ggagaccacc
     6001 gcaaaagcgt ccggtggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
     6061 gcaacctccg tgaaaatgaa taataatccg catatattgc atgtacaaca tgtcgggtaa
     6121 tgagataagc gggccggctt caggcgttac cggggaatat cagaaaagtg tcatggttat
     6181 gtgatgattt tgtccgggat tttacttcag agaaacgggg ggctgttgac tcaacaaaat
     6241 aatcacaaac tcaactgacg ggttcacgat gttattaagg acaggctcgt atattacatc
     6301 gagtaacaaa aaaacacctg atatgcaaag tcaaaccaat atttgtaatg tctttttatt
     6361 cacaataata aataactcaa taaacattat tgaaatgaat gatggtttgt ataaggaata
     6421 tacgctaccg ccccaccctg ataacatgat ctgcacattg ctatcttaac gtcgttagac
     6481 gctggcttaa tagtgaatat tttaaattag gaatttatca agttc
//
LOCUS       DAZGHF010000205         6425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGHF010000205 DAZGHF010000000
VERSION     DAZGHF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.206"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..837
                     /locus_tag="ACHK3A_004647"
     CDS             <1..837
                     /locus_tag="ACHK3A_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO8742626.1"
                     /translation="LVKSESTVLRESICAVIAGKDCYQADTLSRLPGVKIISRFGTGI
                     DNIDLHTARQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHG
                     CELQGKRIGLLGYGNIGKTLAKRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFM
                     QSHVIIVLLPFSSELENFISHKYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFA
                     ALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKN
                     VL"
     gene            complement(1139..2515)
                     /locus_tag="ACHK3A_004648"
     CDS             complement(1139..2515)
                     /locus_tag="ACHK3A_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO8742627.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LSGQFRNAALRLERLAPQGAETIITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(2515..2943)
                     /gene="tssE"
                     /locus_tag="ACHK3A_004649"
     CDS             complement(2515..2943)
                     /gene="tssE"
                     /locus_tag="ACHK3A_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO8742628.1"
                     /translation="MATPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHSLMRQIEETLLRYEPRLNQIQVELLPQPGPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(2947..3483)
                     /gene="tssJ"
                     /locus_tag="ACHK3A_004650"
     CDS             complement(2947..3483)
                     /gene="tssJ"
                     /locus_tag="ACHK3A_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO8742629.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(3464..4543)
                     /gene="tssG"
                     /locus_tag="ACHK3A_004651"
     CDS             complement(3464..4543)
                     /gene="tssG"
                     /locus_tag="ACHK3A_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO8742630.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(4507..6267)
                     /gene="tssF"
                     /locus_tag="ACHK3A_004652"
     CDS             complement(4507..6267)
                     /gene="tssF"
                     /locus_tag="ACHK3A_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO8742631.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSNFQVLSRPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGTQQFWLRLPGQERRVLDAHFS
                     PMGFDDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLENVIWPEGMSGFEIDV
                     VLSENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDSDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLSAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSTGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1498 a         1709 c         1705 g         1513 t
ORIGIN      
        1 ctggttaaat cagaatccac agtattacga gaatctattt gcgcagtcat tgccggaaaa
       61 gattgttatc aagcggatac gttatcgcga ctaccaggag tgaaaataat atcgagattc
      121 gggacaggga ttgataatat tgatcttcat acggctcgac aatcaggtat tgtggtcaat
      181 aatgctgttg ggattaattc aaatgcagtc gcggaattta tcatcggtct tatttttgcg
      241 agcatgagaa atatccccgg tagctatcat gcaatgcaaa atggttattg gggtgagtcg
      301 catggctgcg agttacaagg taaacgtatt ggcctgcttg gctacggcaa tattggcaaa
      361 acactggcga aaaggttatc tggttttgat gttgagcttt tagcttttga caaacaaccc
      421 gactatcagg ttgctgacaa agctggagtt cagtttgtat caattgaaga tatctttatg
      481 cagtcgcatg tcatcattgt tcttttgcct ttttcttctg agctggagaa cttcattagt
      541 cataaatatt tgtcgatgat gcgcaatggt gctctgatca tcaatgccgc aaggggaaag
      601 ttgctggatg agggcgcttt acttcaggtg attgaagaac gaaatgtgtt tgcggcgctg
      661 gatgtgttta gctctgaacc gttggcgcaa tttagcccac tgctacatgc caaaaatatc
      721 attacaacgc cgcatatcgc ggccgccacc gttgagtcat accagcaaac cgggatacac
      781 gttgcccagt cgataattga ttacttcgca ggaagggaga taaaaaacgt gttgtgagtt
      841 cagcgaacaa agattttaaa tacaaaaaaa ccgcaatttg tagaacacat tgcggtttgt
      901 cagcattcta ataaattggt tgcgggggcc acagtcaaga ggttaatcca atcttttgaa
      961 acttaaggta ttcactgttc ccgaacccaa gctatctacc gaaatataca ctcattatct
     1021 gatgtgaatc aacgagtaga tcgaatggcc tgttcctctc tttttttgta cacagagact
     1081 ccattttaaa aaatggcaag gtcacatatc ccttattggt acataaatcc cccgatgttt
     1141 actgactttg ttccaccttc gtatccagta aaatgacata gcgattcaac aactgattta
     1201 accgggtatc aatttgcgtc aacagcgttg gcgaaacctc tccggtctgt ttttgctctt
     1261 ccagttgccg aagcagttct tccagcggcg gctcctttaa cgactgccgg atgccgtaca
     1321 ccgccgtttt tagttccgaa ccggtcagat atctcccctt acgttcatcc agtgcatcca
     1381 gctgcgccga caggcgctgt agctggctct gcgcctgagc atagccattc agtgcctcag
     1441 cagacagggc gctcagctcc cgctgtttgc gccactgcgc gttcagcgcc ctgacctgag
     1501 gattatccgg ccacagggca tcaagctggc gtagctggcg atatccctgc tccagtggcc
     1561 agcgggcagg caatttgttt aactgctcca gttgcatctg acttgccgca attgttctgt
     1621 cctgagccag cagcgcctta tcctttacat ccagctttgc cagttcgccg ctctccagcg
     1681 actgcggcaa cggcatgaca gacagttgta ttcgctggat tagcgcgtcc ggctgattaa
     1741 gctgcgacca gccccataat cctgttccgc cgagaccggc catcacaacc attccggcca
     1801 taaatatcgg ccatgtccgc tgccgggaga tttcagggga tggcacgggt ggactggcat
     1861 ccttatcgtt ttgctgaacg gatctggttg caaaaaccgg ctgtggactt tcctctgact
     1921 ttttcggttg attcatttcc cggcggggta attcaggggg agtgataatg gtttccgctc
     1981 cctgaggcgc cagccgttcc agacgcagag ccgcattgcg aaactgcccg gacaatacgt
     2041 ccagcttcgt catatgccac agttcacact gttgcagcgt ggtttcgatg gcggaaagat
     2101 gctgtacaca acggtagatc tgcggcagat cctgatactg aatatccagc gttctcagtg
     2161 cctgctgcat tttttctgtg acccagctga gcagtgatat tctggaatgt acctgtaccg
     2221 gccagaagtc agcccagcgc tggagcagta tatccagtga accgagtccg tctgccatcc
     2281 ctgccagccc ctgccgtctg gttattgcca gcacgtaaca gaccagcgtc tgtagttcca
     2341 cgccattttg ccggaacagc gcgaggcaga gtttttccac ctgacgccag tccacatccg
     2401 gtcgggccgg gtgagtcagt ttactcattt cagcctgcag ttcaccggat tccttataag
     2461 tacagggatc gccgcccatg acgaactggc tgataaaatt cgcgttactt gccatcagta
     2521 cgcccgctcc tgttgtttga gatgacgcag aacaattcgt ccttccggag aaaatacgcc
     2581 atcaaagcgt atccagccgg tatcgggaag ctgcgcgtgg atcaggtaat taagatgccc
     2641 cggaccgggc tggggcagta actccacctg tatctgattc aggcgtggtt cataacgcag
     2701 cagcgtttct tcaatctggc gcatcaggct atgaataccc gcagccatcc cctgatggat
     2761 caggctgaga tccggcagac cgtaatccgg caaatgcgcc agtgtgcctg cccgacagtt
     2821 gataatgcgt tgcatattgt ccatcacgga aaggataagc tgatcccgct cactgacctg
     2881 ttccagcggt aactcgccgc tgaaactgaa cgtcagcatt tcatataagg aaggtgtcgc
     2941 catcggttat ttatccttga gaggcagcag tcgcaggtcg cttctcatca gttcaatcga
     3001 gcgcggcttg tcggcctcca gttcgtccct tttgatcacc agacgccagc tgtcgctttt
     3061 ttcatccggg tgataaaact gcccaataat ggcgacaaac tgcgtctctt tatccagcgg
     3121 cacattcagg gatgccgtgc cttcaggttt tacccagacg gtgtgttttg ccagtacgtc
     3181 cttctccagc gtcttctctt cctgcataaa cagggtgtca taatccgcct tatcaaaatc
     3241 ttcgcgggtt ttcagttgcc agacatgcac catcgtggaa agcggcgtat cttccgcatc
     3301 cgtattcagg gcgctgcggg agaagaaatc gagatgcagt attttgattt gtttgtagaa
     3361 aacggctgag gccacgcttt tcgattcatc caccactttc tgaatcagcc cgcagccact
     3421 cagaacaacc gtcagcatcg ccatacagag caacgccgga cgtttaaaac gaataggaaa
     3481 catgagtcat actctccctg ttacgaatgg aaaaagcggg tattaatccc tgataacgcc
     3541 ccagactgac cgtcactgtt ccggtcgcgg gcgcggcaaa ggaggcgcga aggatgccgg
     3601 tacggctaat ctgtacacgc tgttttccca gccgggcagc aggcaacagg ctgacgggca
     3661 gcgtcagttg cagacgggcc tgacagcgcc agcccagata aacgcgcagc agcaccagca
     3721 aatcgctgtg cagttgcccg ccgggcagcc agccacgtgc ttctgcggca tcttcggtat
     3781 acagtttcag cagcaactgg ctgttgacgt cggtaccggt acgccccagc agcgttcgcg
     3841 ttttcaggct gatgcgtgaa ttttttgaca gacccgcagg ggccgggaga attacaggct
     3901 gcggatcgtg aggcaccacc gtcgctttgg tcagcggggc aagcagccct accagtgccg
     3961 tgacgccttc cgccgtgcgc gtcggcaggc gcatcacgct gagcagggcc agaaaacggg
     4021 ataccggcgt ggcgatatgg ttctcgctgc cgggaatacc cagcccgatg agtcccagca
     4081 gacaacggga aatgtcatcc acggcacctg cttcaaacga cgccgggtag ttatatttgc
     4141 gccagatgcg gtagtactga gtaataaagc gatggttaaa aatatcgaga aacgccatca
     4201 ctgcgtcatg tccgtcatga cgctgggtaa tgtagtccag ataggcggtt ggcagcggcg
     4261 aatccacgcc gtacaggccg agaaaagtgg tgcgcaccgt ggggggcgcg tcgggattgc
     4321 ccacgtcacg ttcaacgttt tttaactcgc tgaccggaaa gcccatacct ggatgaggac
     4381 gaaagcgcac cgcatcggtg gcggggctgt cggtactgcc cagcggcgcg ttctgctggc
     4441 tctgttccag caactggcaa aaacggtaaa aattgatccg cgcaatatcc ggggccagcc
     4501 ggtcggtcag cccggtacgc gctggctgtg atgctctgtc cattgcaggc actttcctgt
     4561 gggttgcaga attaaggtca gttgagtgaa taagtggata tcggcataca gtgcaaagaa
     4621 gcggtgcagc agttcgccaa acagcgtgat atcaccctct ccggcaaagc cggtgctgtc
     4681 cagcgtcacc tgaatatcca cgccacgcag cagaaagcct ctttcaaaac gctgtatcag
     4741 gctgtgctga acgtcgacaa tcgccgacag acggcggcgg ttcatctcgc tttcggtcca
     4801 gtcgtacagc gccagcgtgc cgcgcagaat ttcggcgtta tccatcattg aaagaaagtt
     4861 agaaccgagg tggctgagca cccgccagtg gaagcggtca cgcgccgggg gataacaggg
     4921 ctgcgtcggg gcgcacaggt tgcgcacccg caggacgttc tgtgaggcat gaactggcgt
     4981 atcaagcagc gtgctctgta aagccttacg gggaagctgt ccgttagtgc cggtcaggct
     5041 taaagagagc gtttcatcct ccagcatccg gtcggaatca aacgcatcgc cgcccagaat
     5101 cagccaggta tcatgcaggc cggaagggcc gcgcttaacc cgcgtatggt aatagcgttc
     5161 cggtgcatcg tggcgcagca tcccgccccg gtgacgaaag ctggaaaacg ggacatacgc
     5221 ctgactgccg gtatggcgcg aagaaataat gttgtcgacg gaataaatct cggtatgccc
     5281 gtcctgaata cgcatcgggc gcagcagaaa ttcgttttcc agtggcgaaa ggtgtaacgg
     5341 atcggcttcc agcgggaaca ggttgatgac cggcacgcag tgcagacgga tattatcgct
     5401 gtcaaacggc aggtcgtgag gccagttctc gctcagcacc acgtcaatct caaagccgct
     5461 catcccttcc ggccagatga cgttctccag cccgttcagc gccacaaaca taaacttttc
     5521 gcggaaggtg aaatactcca gcagcaactg gtagccgctg aacgccgact cgccttttgg
     5581 ccacagtcgg tcattgtcat caaaacccat cggggaaaag tgcgcgtcca gcacacggcg
     5641 ttcctgcccc ggcagacgca gccagaactg ctgggtgcca agcgttaaag cccggtgcag
     5701 cgcacaggcc accgggctgt cggcattgag atagagcggc aaacgggata gatcgatctg
     5761 tgaccagtca ccaaccagcg ggccgcattc aaagcgcaga cggataaccg agcgcccgtc
     5821 cggttcatat tgcaatgaca cgtccggcag cgccagcgga tgcagcgtga tatcccgcgt
     5881 agcactgtag acacagcggg ttctgcgctc gccaacagga cgcgacaata cctgaaaatt
     5941 actcagtgtt tcgctctgct tcatctgccg gtgatctgtc gacagttcaa ccacggaaag
     6001 tgacgggatg gttcgcaggt agtgaggcca cagcaggctg accagcccct cggtcagttc
     6061 gggcaggtcg tcatccagct tttcacgcag gcgacccatt aaaaaagcga agccttcgaa
     6121 caggcgctcc acatagggat cgcgtgcgcc gggtttatca agattgagca tcgcggcgcg
     6181 gtcggggtaa gcctgcgcaa attctttccc cgcttcacgc aggtagcgca tttcggcttc
     6241 gtagtagcgt tgggttagat cgtccatcgg agggattatc ttcctttcat tgaaagtgaa
     6301 tatcatgcag gtgtagaagt aacatcatag ttaaatttat tctatgtgaa tatattatgg
     6361 atagttgctg acttattgat tttaataatg atgtattctt gtcttttctg aaccaaatta
     6421 aattt
//
LOCUS       DAZGHF010000206         6216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGHF010000206 DAZGHF010000000
VERSION     DAZGHF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.207"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            226..2073
                     /gene="ldtD"
                     /locus_tag="ACHK3A_004653"
     CDS             226..2073
                     /gene="ldtD"
                     /locus_tag="ACHK3A_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO8742632.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVVGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2254..2802
                     /locus_tag="ACHK3A_004654"
     CDS             2254..2802
                     /locus_tag="ACHK3A_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO8742633.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2829..3476
                     /gene="gloC"
                     /locus_tag="ACHK3A_004655"
     CDS             2829..3476
                     /gene="gloC"
                     /locus_tag="ACHK3A_004655"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO8742634.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3527..4717)
                     /gene="aspC"
                     /locus_tag="ACHK3A_004656"
     CDS             complement(3527..4717)
                     /gene="aspC"
                     /locus_tag="ACHK3A_004656"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO8742635.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETIDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(4902..5990)
                     /gene="ompF"
                     /locus_tag="ACHK3A_004657"
     CDS             complement(4902..5990)
                     /gene="ompF"
                     /locus_tag="ACHK3A_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO8742636.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1570 a         1522 c         1579 g         1545 t
ORIGIN      
        1 cgaaaactcc gtttctgcac taaaaaagtg gcacattacg gcgcgctttt ctttaaactt
       61 ctttacatta ggttatgtaa aaacgtatcg gcgtttatat actgaagata agcctgatga
      121 gtaacaggct tgctcgtcat actttcgtga gtattggcgt tgtacaggca agtcgtaaaa
      181 taacagcctg gctattcaga gtatgataaa aacagggggc aagggatgtt gcttaatatg
      241 atgtgtggtc gtcggctgtc ggcaatcagt ttgtgcctgg ccgtaacatt cgctccactg
      301 ttcaatgcgc aggccgatga gcctgaagta atccctggcg acagcccggt ggctgtcagt
      361 gaacagggcg aggcactgcc gcaggcgcaa gccacggcaa taatggcggg gatccagcca
      421 ttgcctgaag gtgcggctga aaaagcccgc acgcaaatcg aatctcaatt acccgcaggt
      481 tataagccgg tttatcttaa ccagcttcaa ttgttgtatg ccgcacgcga tatgcaaccc
      541 atgtgggaaa accgtgatgc tgttaaagcc ttccagcaac agctggcaga ggtggcgatt
      601 gccggtttcc agccgcagtt taataaatgg gtagagttac tgaccgatcc tggtgttaac
      661 gggatggcac gcgacgtggt gctctctgat gcgatgatgg gctatctcca tttcattgca
      721 aatattccgg tcaaaggcac tcgctggcta tatagcagta aaccttatgc gctttcaacg
      781 ccgccgcttt cggtgattaa ccaatggcag ctggcgctgg ataaaggtca attgcctacg
      841 tttgttgtag gattggcacc gcagcatccg caatatgcgg cgatgcatga atcgttactg
      901 gccttactca gtgacaccaa accgtggccc caactgaccg gcaaagcaac gttgcgccca
      961 ggacagtgga gtaacgacgt accggcgttg cgcgaaatat tgcaacgtac aggcatgttg
     1021 gacgggggac cgaaaattac gctacctggc gatgacacgc caactgacgc ggtagtcagc
     1081 ccatccgctg ttactattga aacagcagaa actaagccga tggacaagca aactacgtct
     1141 cgtagtaaac ctgcgcctgt cgttcgtgcc gcctacgata atgaactggt ggaagccgtt
     1201 aaacgttttc aggcatggca aggattgggg gcagatggtg ctattggccc ggcaacgcgt
     1261 gactggttaa acgtaacgcc cgcccagcgt gctggcgtgt tggcactcaa catccagcga
     1321 ttgcgcttgc tgccaacaga gctttctacc gggatcatgg ttaacattcc agcctattcg
     1381 ctggtctact atcagaacgg caatcaggtg ctggattcgc gagtcattgt cggtcgcccc
     1441 gatcgcaaaa cgccgatgat gagcagtgca cttaacaacg tagtggtaaa cccgccgtgg
     1501 aacgtaccac caactctggc acgcaaagat attctgccga aagtgcgcaa cgatccggga
     1561 tatctcgaaa gccatggcta tacggtgatg cgcggctgga acagcagaga agcgattgac
     1621 ccatggcagg ttgactggtc tacaatcacg gcctcgaatt taccgttccg cttccagcag
     1681 gctccaggcc cacggaactc gctggggcgc tataaattca atatgccgag ttcagaggcc
     1741 atttatttgc atgacacgcc gaaccacaat ctgttcaagc gtgatacacg cgcattgagc
     1801 tcaggctgtg tacgagtgaa taaagcttcc gatctggcga atatgctgtt gcaggatgca
     1861 ggctggaatg acaaacgtat ttctgatgcg ctgaagcagg gcgatacgcg ttacgtcaat
     1921 attcggcagt cgattccggt gaatctctac tatctgacgg cctttgttgg tgcagatggt
     1981 cgtacccagt atcgtacaga tatttacaat tatgatctgc ctgcgcgatc cagctcgcaa
     2041 attgtatcga aagcggaaca attaatcagg taaatgaagc agttcgggga aatcagttgt
     2101 cgtaataatt agcatgattg ggggcaattc tctgcagccc ccgtcactgc tggggttgag
     2161 tcatcttgac gtctgcttta cgggcggtta aggtgcctct tgtgcgccag aagtgcatat
     2221 aaacgataac attgacctgt agacttgatt atcatggaca aattcgacgc taatcgccgc
     2281 aaattgctgg cgcttggtgg cgttgcactc ggtgccgcca tcctgccgac ccctgcgttt
     2341 gcaacactct ctaccccacg cccgcgcatt ttgacactca ataatcttca taccggagag
     2401 tcaatcaaag cggagttttt cgatggcaga ggctatattc aggaagaatt ggcaaaactt
     2461 aaccattttt tccgcgatta ccgcgcgaac aaaataaagt ccatcgaccc aggattattc
     2521 gaccagttgt atcgcctgca agggttgtta ggtacccgca aaccggtgca actcatttcc
     2581 ggttatcgtt ccattgatac caacaatgaa ctacgcgccc gcagccgtgg agtagcgaag
     2641 aaaagctatc acacgaaagg gcaggcgatg gatttccata ttgaaggtat cgcgttaagc
     2701 aatattcgca aagccgcgtt atctatgcgc gcaggtggtg taggatatta cccacgtagt
     2761 aactttgtgc atattgatac cgggccagca cggcactggt agcaattgct taacgaaaca
     2821 ggggcagtat gaactatcgt attattccgg tcaccgcatt ctcccagaac tgttcattga
     2881 tctggtgtga acaaacccgc ctggccgcac tggtcgatcc tggcggcgat gcggaaaaaa
     2941 tcaaacagga agttgacgcc agcggcctga cactgatgca gatcctgctg acgcatggtc
     3001 atctggacca cgttggtgca gcggcggaac tggcgcaaca ttacggcgtg ccggtttttg
     3061 gcccggaaaa agaagatgag ttctggctgc aaggcttgcc tgcgcaaagt cgtatgtttg
     3121 gtctggaaga gtgccagccg ctgacgccgg atcgttggct gaacgaaggc gataccatca
     3181 gcatagggaa tgtggcttta caggtgttac attgccctgg gcatacgccg ggtcatgtcg
     3241 tgttttttga tgatcgggca aagctgctga tttctggcga cgttattttc aaaggcggag
     3301 tagggcgcag tgacttcccg cgtggcgatc ataatcaact gatttcttca atcaaagata
     3361 agttgctgcc actgggggat gacgtgacat ttattccggg tcacggacca ttatccacac
     3421 ttggttatga gcgcctgcat aatcccttcc tgcaagacga aatgcccgtc tggtaaaggc
     3481 gcataaaaaa gcccgctttt taagcgggct tcattgtttt taacgcttac agcactgcca
     3541 caatcgcttc gcacagcgga gccatattat ctggtgtcat cccggccacg tttacgcgac
     3601 cagaagcaac agcatatacg ccaaactctt cgcgcagacg cagcacttgt tcttttgtca
     3661 ggccactgaa ggagaacatg ccgttctgtt tgatgataaa gctgaagtcg cgatttgcgc
     3721 ctttttcctg cagcgtattg acgaacaact gacgcatacg ctgaatacgc tggcgcatat
     3781 cagtcagttc ttgttcccaa atcgcacgta acgcatcgtt gctcaggatg gtggcaacaa
     3841 cagaagcgcc gtgtgctggt gggttagagt agttagcgcg aatcgccgct ttcatttggc
     3901 tgaatgcgcg atcaatggtt tcactgtcag cagcaaccag agtacaagcg ccaacacgct
     3961 cgttgtacag gccaaagttt ttagagtagg aactggcaac aatcagctct ttatgcatag
     4021 ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc acgggcaaac ccctggtaag
     4081 cgaagtcaaa cagcggtaac cagcctttct caacggaaag ttgtgccagt gtctgccatt
     4141 gttccagcgt agggtcgata ccggttgggt tatggcagca gccatggaac agcaccacgt
     4201 cgccagcctg agcttcgttc aggctgttaa tcagtgcatc gaagtcaagg gtgtgatttt
     4261 ccgcatcata ataagcgtat tcacgaactt ccagacctgc agagttaaag acgctcttat
     4321 ggttcggcca gcttgggttg ctcacccaca cacgcttaac gctggtattt tttgccagga
     4381 aatcggcagc cacgcgtagt gcgccagtgc cacccggagt ctgtgccgtg cgagcacgtt
     4441 tgtcattgat cagggcgcta cctttaccaa acagcagttc ctgagtgcag cgaccaaatt
     4501 cagggatgcc gtcaatgccg aggtaatttt tggtggtttc attttcgagc agatactgtt
     4561 cagccttttt cacactggtc agtaccgggg ttttgcccgt ctcatcttta tagacaccaa
     4621 tcccgaggtt aattttgccg ggacgttcat cggcacgaaa cagatcggcc aggcccagaa
     4681 tcgggtcggc aggagcggcg gtaatgttct caaacatgac gaggttccat tatggttaca
     4741 gaagggaagt ccgctatcag ggtaacggga gatttacaaa attccaacta ttactgatga
     4801 aaacgcaggc tgtttttgca agacgtgaga ttgctctgga aggcataaaa aaaacaggac
     4861 caaagtcctg ttttttcggc atttaacaaa gaggtgtgct attagaactg gtaaacgata
     4921 cccacagcaa cggtgtcgtc agaacctacg cccagtttgt tgtcagaatc gatctggttg
     4981 atgatgtagt caacataggt ggacatgttt ttgttgaagt agtaggttgc gcccacttca
     5041 aagtagttaa ccagatcaac atcaccgata ccttctacgt ctttcgcttt agatttggtg
     5101 taagcgatgg acggacgcag accgaaatcg aactggtatt gcgcaactaa cagaacgtct
     5161 tgcgttttgt tggcgaagcc gctgatgttt gtaaatttat tagtgatcgg cgtagcgtta
     5221 cgggtttcac cgtagttcgc tgccaggtag atgttgttcg cgtcatactt cagaccagta
     5281 gcccactgtt cagctttttt accgttgcca agaggttgag cttcttgcag gttggtacgg
     5341 tcagcggcgc cataagcacc aacgatacca aagccttcgt attcgtagct gatagaaccg
     5401 ccaacaccgt cgccgttaga gcggcgtgca gtgtcacgct cgtttttacc caggtactga
     5461 acagcgaagt tcaggccatc aaccagacca aagaagttgg agttacgata ggtagcaacg
     5521 ccgccaacac gaccaacgaa gaagtcatcg ctgtatgccg tgtcaccgcc aaactcaggc
     5581 agcatatcgg tgtaacccag tgcatcataa accacaccgt agttacggcc gtaatcgaaa
     5641 gaaccaacgt cagcgtattt aagacccgcg aatgccagac gcgttttgtt accagtttga
     5701 gcgtcagcgc cttcagagtt gttaccctgg aagttatatt cccactgacc ataaccggtc
     5761 agatcggaat tgatttgagt ttccccttta aaaccaagac gggcataggt catgtcacca
     5821 ttgccaccgt aactgttttc accgttaccc ttggagaaat aatgcagacc gacagcttta
     5881 ccgtacagat ctactttgtt gccatctttg ttatagattt ctgcagcgtt tgcagtacct
     5941 gctactaaca gagcagggac gatcactgcc agaatattgc gcttcatcat tatttattac
     6001 cctcatggtt tttttatgac acctgccact gccgtcaata agttctgtca ataaaaattt
     6061 acggaactat tgatgagagt ttggtgtctt tatgtgtctg caggcatctt tccattcaaa
     6121 ctaacgtttc gctaccgtga aagtgctaca aagataaaga tttggtttca aaaagaaaaa
     6181 atatgtaacc aaaagtaaaa tttaaggaac tttgtg
//
LOCUS       DAZGHF010000207         6113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGHF010000207 DAZGHF010000000
VERSION     DAZGHF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.208"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..411
                     /locus_tag="ACHK3A_004658"
     CDS             <1..411
                     /locus_tag="ACHK3A_004658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742637.1"
                     /translation="QGTDDLAVALLIIYRRYPVVTKKKDKINRDVVISLRLTEKEFGD
                     FVDDIKKSQLSRSAFFRKLILEKSSSVVINVNNTAPLVYHFNKAGNNLNQLAYKVNSA
                     FLMGRISESLYVKVLNCLVDIRELLMTGVNNNAR"
     gene            401..3949
                     /locus_tag="ACHK3A_004659"
     CDS             401..3949
                     /locus_tag="ACHK3A_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO8742638.1"
                     /translation="MLVRFSGHNNGAKEYLEEGKKNGRELTRDELDTRITLYGDLELT
                     QKIYQSIPDNDQERYVSITHSYFEDDISVDVMKDIFYEYKNFLMCVYRDDEFNIYAEA
                     HIPKIKYSEDKKTGELIERKPHVHIIIPEVNLLSGNLINPRGDYRVNEKYFEAVQEYI
                     NQKYNLISPRERVRANPKNVSDILSRYKGDDFRSKNREFKEQLVRDIVEENILSRKDF
                     YTHVSSFGDIRIRNQGKENEYIAVKLPGDKKFTNLKETIFNDDFIVRRELKKPPLEKS
                     VIQQLLHEWPQRAMELKYIDKANSDFRKKYYSSSQEEKIKILAQQETAFYQKYGERYD
                     VFPAEWKTNHKSGINEAQTRGTTFSPNGLQSMSGGEMATGWQTGNTGSSLFLSTDALV
                     FMGQSQSSGRDSGLRCDVHSGGRGRSGNQRGSGQPAITGTNATVHSRRSTRTQYGNRY
                     AGGLLAGDVSIPVYARNKPGGATIQDVIRRSEILLRPPPGKSNISVNRFNGDIPQMGK
                     NAAYVAAHFVRQYEQNKILPNQRQALWDIRQLYQSTRSFIWQDERLTRKERSQYLSVL
                     AFEHVKARQKIINSSQQENTMASEDIRKLIKKKPRLSNNEITGKDERKIPPAGKRFSR
                     IVHTLKNRIEGEDKENSYAKSVSAADLYTRKSKLSKNVHYLDKNSNRTLFVDTGNAIA
                     IRKDGMSPAALGIALELAKERFGSTLTIKGNEEFRNQVIDIVARNHPDLHFSDKAMNR
                     QLEERRAEIAAERDGMTIEGVSKTSTSKEQEKEHEQAADTKNQTNVKESSEPHESPES
                     RTVVKLEGKLIDHGKAPLDNKKGAEQSYFVTVRGRDGKETTHWDSDLQNVMKGKRRGT
                     EISLELKESNPVQVSTRDHLGNLSIREAVRQTWTLNKMSVGQEQKREQSLEKIQQNQS
                     PIPARTQTAVDLILANPVLRQMAMTAEQKEREFTALQADKESVREQTDQEIRNRLGSG
                     DQSREPLAVILSDDELRGMALSMEQKMREMATPVDKLDAVKAEINSEIAARVARNSSI
                     PVPVSEVSATVAQKEIHREAESRGNKDKVHEGVLLDHGRAAYKFKPDISKPEDERDDS
                     YFVKLKMDNGKTRTLWGVGLESAVAGYSKGERLRLEDKGTEKVQWIERMKDGREIEKN
                     GDRRVWDCRSLTRESEVMKGFRVSGMPERDDGPDVA"
     gene            4353..4616
                     /gene="repA"
                     /locus_tag="ACHK3A_004660"
     CDS             4353..4616
                     /gene="repA"
                     /locus_tag="ACHK3A_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO8742639.1"
                     /translation="MSDAVRSATSSEARPKRAYRKGAPLSVNERKRLSLNRKRDTHKA
                     LNVFIRNELKDILERFCQDKHLTLGEGIELLIQQEKERAKNGE"
     gene            4839..4913
                     /gene="tap"
                     /locus_tag="ACHK3A_004661"
     CDS             4839..4913
                     /gene="tap"
                     /locus_tag="ACHK3A_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO8742640.1"
                     /translation="MPGKVQYFFLCSLLLRIVSAGWCD"
     gene            4906..5763
                     /gene="repA"
                     /locus_tag="ACHK3A_004662"
     CDS             4906..5763
                     /gene="repA"
                     /locus_tag="ACHK3A_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO8742641.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALFSSLDI
                     SEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
BASE COUNT         1893 a         1199 c         1506 g         1515 t
ORIGIN      
        1 caggggacgg acgacctggc ggtggcattg ttaattattt acaggaggta tcctgtggtc
       61 actaaaaaaa aggacaaaat taacagggat gtcgttatat ccttaagatt gacagagaaa
      121 gaatttggcg attttgttga tgatataaaa aaatctcaac tcagtcgttc tgcatttttc
      181 agaaaactga ttcttgaaaa atcatcatct gtcgtaatta atgttaataa tacggctcct
      241 ttagtatatc actttaataa agctggtaat aatcttaacc agcttgctta taaggttaac
      301 tcggcttttt tgatggggcg tatttctgaa tccttatatg taaaagtatt aaattgtctt
      361 gttgatatta gggagttatt aatgacagga gttaataata atgctcgtta ggttttctgg
      421 tcataataat ggagcaaaag aatatcttga agagggtaaa aaaaatgggc gagaattgac
      481 tcgtgatgag ctggatacca gaattacttt atatggtgat ttggaattaa cccaaaaaat
      541 ttatcagtct atacctgata atgatcagga gcgttatgtt tcaattacac attcgtattt
      601 tgaggatgat atttctgttg atgtgatgaa agatattttt tatgagtata aaaactttct
      661 tatgtgcgtg tatagggatg atgaatttaa tatatatgca gaggcacata ttccaaaaat
      721 aaaatactcg gaagataaga agaccggtga attgatagaa agaaaaccac atgttcatat
      781 tattattcca gaggttaatc ttctttctgg taatttaata aaccccaggg gagattacag
      841 ggtaaatgaa aaatattttg aggctgttca ggaatacata aaccaaaaat ataatctgat
      901 ttctccccga gagcgtgtca gagccaatcc aaagaatgtg tctgatattt tgtctcgtta
      961 taagggggat gatttcagaa gtaagaacag agaatttaaa gaacaactgg tcagggatat
     1021 tgttgaagaa aatattttat caagaaaaga tttttataca catgtatctt catttggtga
     1081 tatccgaatt cgtaatcagg gtaaagaaaa tgaatatatc gccgttaaat taccgggtga
     1141 taaaaagttt accaacctga aggagacaat atttaatgat gactttattg tgagacgaga
     1201 actgaagaaa cctccactgg aaaaatctgt cattcagcag cttttgcatg aatggccaca
     1261 gcgagctatg gagcttaagt acattgataa ggctaacagt gatttccgaa aaaaatatta
     1321 ttcatcctcg caggaggaga aaataaaaat tcttgcacaa caagaaacag cattttatca
     1381 gaagtacgga gaaagatatg acgtattccc tgccgaatgg aaaacaaatc ataagtccgg
     1441 tattaatgaa gctcagacca gaggaactac cttctctccc aatggcttgc aaagtatgtc
     1501 cggtggcgaa atggcaactg gttggcaaac tggaaacacc gggagttcgt tgtttctgtc
     1561 cactgatgca cttgttttca tgggacagtc gcaatccagc ggaagagatt ctggactgcg
     1621 atgcgatgta cattcaggag gaagaggacg atctggtaat caacgaggaa gcggacaacc
     1681 cgctattacc gggacgaatg ccaccgttca ctccagacgg tcaacaagaa ctcagtacgg
     1741 aaatcgatat gccggaggat tactggccgg ggatgtgagt attcctgtgt atgcgcgtaa
     1801 taaacccgga ggggcaacaa tacaggatgt aattcgccgt agtgaaattc ttttacgacc
     1861 acctccagga aaatcaaata tcagtgtgaa caggtttaat ggtgatatac cccaaatggg
     1921 gaaaaacgca gcttatgtag ccgctcattt tgttcgtcaa tatgaacaaa ataagatatt
     1981 acctaatcaa cgtcaggcat tgtgggacat tcggcagctc tatcagtcaa cccggagttt
     2041 tatctggcag gatgaacgat taactaggaa agaacgctca cagtatctgt cagtactcgc
     2101 ttttgagcat gtaaaagcgc gtcagaaaat cattaatagt agtcagcagg agaatactat
     2161 ggcaagtgag gatatccgca aacttattaa gaaaaaacca cgcctgtcaa ataacgaaat
     2221 tacagggaaa gacgagcgga agataccacc ggcaggaaaa cgtttttcaa gaatagttca
     2281 tacactgaaa aaccgaattg aaggtgaaga taaagagaat tcatatgcaa agtctgttag
     2341 tgcagcggat ttatatacca gaaaatcgaa actcagtaag aatgtgcatt atcttgataa
     2401 aaacagtaac aggacattat tcgttgatac aggaaatgcc attgctataa gaaaagatgg
     2461 tatgtctccg gcggcgctgg ggattgctct tgaactggcc aaagaacgtt ttggcagtac
     2521 actgactatc aaggggaatg aagaatttcg taaccaggtt attgatattg tcgccagaaa
     2581 tcacccagat cttcattttt ctgacaaagc gatgaatcgg caacttgaag aacgacgggc
     2641 agaaattgcc gctgaacgtg atgggatgac aatagaaggc gtttctaaaa cgagtacgag
     2701 taaggaacaa gagaaagagc atgaacaggc agcagatact aaaaatcaaa caaatgtgaa
     2761 ggagtcctca gagccacacg aatcacctga aagcaggaca gtagttaagc tggagggtaa
     2821 attaatagat cacggcaaag cgcctcttga taacaagaag ggtgctgaac aaagttattt
     2881 tgttacggtc aggggcaggg acggtaaaga aaccacacac tgggatagtg atttgcaaaa
     2941 cgtaatgaag gggaaacgcc ggggaacaga gatatctctt gagttgaaag aatctaatcc
     3001 ggttcaggtc agtacacgcg atcatctcgg caatttgtct atcagggaag cggtccggca
     3061 gacttggaca ttaaacaaga tgtcggtggg acaggagcag aaaagggaac agtcgctgga
     3121 gaaaatacag caaaatcaaa gccccatacc agccagaacg cagacagctg ttgaccttat
     3181 tcttgctaat ccagtattac ggcagatggc tatgactgcg gaacaaaagg aaagagaatt
     3241 tactgcattg caggcagata aagaatccgt cagagaacag actgatcagg aaataagaaa
     3301 tcgtctcggt agtggagatc aaagcaggga accgttagcc gtcattttgt cagatgatga
     3361 actacgtgga atggcgttat cgatggagca aaaaatgcgg gaaatggcta ctccagtaga
     3421 taaattggat gcagttaaag cagaaattaa cagtgaaatt gcagcgaggg ttgcgcggaa
     3481 cagcagtatt cctgtgcctg tatcagaagt tagcgccact gtggcacaaa aagaaatcca
     3541 tcgggaagca gagtcgagag gtaataaaga taaagtacat gaaggtgtgc tacttgatca
     3601 tggccgggca gcatacaaat ttaagcctga tataagcaaa ccagaagatg aacgtgatga
     3661 tagctatttt gtgaaactga agatggataa cgggaaaaca cgaacactat ggggagttgg
     3721 tctggagagt gctgtagcag gttatagtaa aggtgagcgt ttgcgactgg aggataaagg
     3781 aacagagaaa gttcagtgga ttgaaaggat gaaggatgga agagagattg aaaaaaatgg
     3841 cgatcgcagg gtttgggact gtagatcgct gactcgtgaa tctgaagtca tgaagggatt
     3901 cagagtatca ggtatgccgg agcgtgatga tggtcctgat gtagcgtaac ttatacaccg
     3961 cgccttatat ccccgccctt aagggcgggg ttttaaaacc agcgggtcga gcaaggcaag
     4021 ttccgaccgt taacgtaatc ccacattcaa gaacttacgg tttcattctt tgttcagcag
     4081 ttgaaaccgt aagtttctgt tcaaatgccg tttcgtgagc tgctcccttt ttgatccggg
     4141 atttgtggag ggtgttagtt aagtaatcac atgatatatc gacacttgtt ccgttcggag
     4201 caggatcttt tctgaatagc atcagatctt tatagctgaa ctggcgatct gtgcgccaac
     4261 atggatccgc cagggtataa ttaagtcgct gatttcaata aagtgtagta tcctctgcaa
     4321 aatgatcctt ttttgatcta tgaggaggct gtatgtctga cgctgtacgt tcggcaactt
     4381 cctctgaagc cagacctaaa cgggcatata gaaaaggtgc accgttaagc gttaatgagc
     4441 gaaagaggtt gtctctcaat cgaaagcggg atacacataa agcgcttaat gttttcatcc
     4501 gtaatgaatt gaaagatatt cttgagcgct tttgccagga taagcatttg accttgggtg
     4561 aagggatcga gctgctaatc cagcaggaga aggaacgagc taaaaatggt gagtagtcac
     4621 ctttatttga tccttttctg gcagagtgat agattacgga cagaaaattc atggctggcc
     4681 acgccgtaag gtggcaggga actggttctg atgtggattt acaggagcca gaaaagcaaa
     4741 aaccccgata acctttctat gtttggcgac gcagaagatt accggggtcc tacaaaaccg
     4801 tatagccaac aattcagcta tgcggggagt atagttatat gcccggaaaa gttcaatact
     4861 tctttctgtg ctcactcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa
     4921 caaacgtatt accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgca
     4981 ggaacgctga agttctgcga aaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt
     5041 gatttcgcca ttcatgtggc gcatgcccgt tcgcgtggtc tgcgtcgacg catgccaccg
     5101 gtgctgcgtc gacgggctat tgatgcgctc ctgcaggggc tgtgtttcca ctatgacccg
     5161 ctggccaacc gcgtgcagtg ctccatcacc acgctggcca ttgagtgcgg actggcgacg
     5221 gagtctgagg ccgggaaact ctccatcacc cgtgccaccc gtgccctgaa gttcctgtct
     5281 gagctggggc tgataaccta ccagacggaa tacgacccga caattggctg taacatcccg
     5341 accgacatca cgttcacgcc ggcactgttt tcctcactcg atatatcaga agaggcggtg
     5401 gcttccgcac gccgcagccg cgtggaatgg gaaaacaagc tgcgcaaaaa gcaggggctg
     5461 gatgcgctgg gtatggatga actgatagcg agagcctggc gttttgtgcg cgagcgtttc
     5521 cgtagttatc aggcagagct taagtcccac ggcatgaaac gtgcccgtgc gcgccgcgat
     5581 gcaggcagga cccgtcagga cattgtcacc ctggtgaaac ggcagctgac acgtgaaatc
     5641 gcggaagggc gtttccgcgg aagcctggag gccgtgaagc gcgaaatcga ccgtcgcgtg
     5701 aaggaacgca tgataatgtc gcggaacaac aactacacac ggctggccac agcctccccc
     5761 tgagttttac ctcatctgaa taatccggcc tgcgccggag gcttccgcac gtctgaaggt
     5821 cggcgacgca caaaaaagca gctcagcaca taaaaaacag cctcatcatc tgctttccgg
     5881 ctcgcaatgc tcccccggtt tttcacgcaa aacacgtctc acagacggag aattccgctt
     5941 atccacattt aactgcaagg gaattctcca taaggttaca atcgttcatg tcataaagcg
     6001 ccagccgcag gcgttacagg ctgcaatgtt tcttttaaac cccagtactg tatttccttt
     6061 aaactactta attacttcca tttaaaaaga aaacctattc actgcctgtc ctg
//
LOCUS       DAZGHF010000208         6084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGHF010000208 DAZGHF010000000
VERSION     DAZGHF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.209"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            214..1632
                     /gene="araE"
                     /locus_tag="ACHK3A_004663"
     CDS             214..1632
                     /gene="araE"
                     /locus_tag="ACHK3A_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO8742642.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            1761..2453
                     /gene="ygeA"
                     /locus_tag="ACHK3A_004664"
     CDS             1761..2453
                     /gene="ygeA"
                     /locus_tag="ACHK3A_004664"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO8742643.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAIIRAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADKRAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(2440..3375)
                     /gene="lysR"
                     /locus_tag="ACHK3A_004665"
     CDS             complement(2440..3375)
                     /gene="lysR"
                     /locus_tag="ACHK3A_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO8742644.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            3497..4759
                     /gene="lysA"
                     /locus_tag="ACHK3A_004666"
     CDS             3497..4759
                     /gene="lysA"
                     /locus_tag="ACHK3A_004666"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO8742645.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAARQQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            complement(4766..5797)
                     /gene="galR"
                     /locus_tag="ACHK3A_004667"
     CDS             complement(4766..5797)
                     /gene="galR"
                     /locus_tag="ACHK3A_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO8742646.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
BASE COUNT         1424 a         1440 c         1658 g         1562 t
ORIGIN      
        1 atcaattaat gaactttatg aattttatct gctttaaaat taggtggtta ataataatct
       61 caataattca acttaatttg aaaattggaa tatccatcac ataacgacat gtcgcagcaa
      121 tttaatccat atttatgcta tttccgacct gacacctgtg tgaattgttc acgtattttt
      181 tcactatgtc ttactctctg ctggcaggaa aaaatggtta ctatcaatac ggaatctgct
      241 ttaacgccac gttctttgcg ggatacgcgg cgtatgaata tgtttgtttc ggtagctgct
      301 gcggtcgcag gattgttatt tggtcttgat atcggcgtaa tcgccggagc gttgccgttc
      361 attaccgatc actttgtgct gaccagccgt ttgcaggaat gggtggtcag tagcatgatg
      421 ctcggcgcag caattggtgc gctgtttaat gggtggctgt cgttccgcct ggggcgtaaa
      481 tacagcctga tggcgggggc tatccttttt gtgctcggtt cgatagggtc cgcatttgcg
      541 accagcgtag agatgttaat cgccgctcgt gtggtgctgg gcattgctgt cgggatcgcg
      601 tcttacaccg ctcctctgta tctttctgaa atggcaagtg aaaacgttcg cggtaagatg
      661 atcagtatgt accagttgat ggtcacactc ggcatcgtgc tggcgttttt atccgataca
      721 gcgttcagtt atagcggtaa ctggcgcgca atgttggggg ttcttgcttt accagcagtc
      781 ctgctgatta ttctggtggt cttcctgcca aatagcccgc gctggctggc ggaaaaaggg
      841 cgtcatattg aggcggaaga agtgttgcgt atgctgcgcg atacgtcgga aaaagcgcga
      901 gaagaactca acgaaattcg tgaaagcctg aagttaaaac agggcggttg ggcactgttt
      961 aagatcaacc gtaacgtccg tcgtgctgtg tttctcggta tgttgttgca ggcgatgcag
     1021 cagtttaccg gtatgaacat catcatgtac tacgcgccac gtatcttcaa aatggcgggc
     1081 tttacgacca cagaacaaca gatgattgcg actctggtcg tggggctgac ctttatgttc
     1141 gctaccttca ttgcggtctt tacggtagat aaagcagggc gtaagccggc tctgaaaatt
     1201 ggtttcagcg tgatggcgtt aggcactctg gtgctgggtt actgcctgat gcagtttgat
     1261 aacggtacgg cttccagtgg cttgtcctgg ctctctgttg gcatgacgat gatgtgtatt
     1321 gccggttatg cgatgagcgc cgcgccagtg gtgtggatct tgtgctctga aattcagccg
     1381 ctgaaatgcc gcgattttgg catcacctgc tcaacgacga caaactgggt gtcgaatatg
     1441 attatcggcg cgaccttcct gacactgctt gatagcattg gcgctgccgg tacgttctgg
     1501 ctctacactg cgctgaacat tgcgtttgtg ggcatcactt tctggctcat tccggaaacc
     1561 aaaaatgtca cgctggaaca tatcgagcgc aaactgatgg caggcgaaaa gttgagaaat
     1621 atcggcgtct gatttcacgg gccggatgtg ctgtacatcc ggcccttttt tcgttaatag
     1681 agattaggca cctggccgtt gaggcgtttg tctcgttcct tattcagcct tgttgcggta
     1741 acacacatca ggagagagga atgaaaacaa ttggtttgct gggaggaatg agctgggaat
     1801 ccactattcc ttactatcgt ctgataaatg aaggcattaa acagcggctt ggtgggcttc
     1861 actctgcgca agtgctgcta catagcgtcg attttcatga aatagaagag tgccagcgtc
     1921 gcggtgagtg ggacaaaacc ggggatattc tggctgaggc ggcgcttggc ttacagcggg
     1981 cgggcgcaga aggtattgtg ttatgcacca atacgatgca taaagtggcg gatgccattg
     2041 agtctcgttg ctctctgcct ttcttacaca tagcggatgc cactggacgt gcaattatcc
     2101 gggctggaat gactcgtgtg gcgctgctgg gtacgcgtta caccatggaa caggattttt
     2161 atcgcgggcg gctgacggaa caattttcca tcaactgtct cattcctgaa gcggataaac
     2221 gggcgaaaat taatcagatt atttttgaag aactgtgtct ggggcaattt accgaagcgt
     2281 cacgcactta ttatgcgcaa gtgattgctc gccttgcaga acagggcgca cagggtgtca
     2341 tttttggctg tacggaaatt ggtttactgg tgccagaaga gcgcagtgtt ctgcctgtgt
     2401 ttgatactgc ggcgatccat gccgaggatg ctgtcgcttt tatgctgtcg tagctgacga
     2461 caaaatagcg tcaagagaag tgaccagttt cggtaatccc gcttgtaaat gctcactaaa
     2521 cgcctgaacc agcgctgatg acgggcggtg cagggggcgg atcaggctaa cggtgaacgg
     2581 aaccgcaata ctgaaccgcc gtaccactaa accgcttgcc gcataatcca gtgcggtgag
     2641 cgggttaacc accgaaacac ctacccccgc ccgtaccatt gcgcagactg acgcggcgct
     2701 gtgggtttct acgatcatgc gtcgtttaac ctgattctca gtaaacaatt gatccagcaa
     2761 ctggcgatag ctgtcagtac gggaaaggct gatgtagttt tcaccgtgaa aatcatccgg
     2821 cgttaatacc ttttttaccg ccagcggatg gcccggaggt aacacacaca cttcatctaa
     2881 agagagtaat tcggtacgtt ctgttcccgc aggcgtatgg agtgtctccg tgagtcctaa
     2941 atcatgacgc tgggccgaga gccactcttc aagtagcggt gattcctggg gcacgatatt
     3001 taagctgaca tcgggataac gtgccagaaa gggttgcagg agctgcggta aaaaagattg
     3061 cgaaaagacc ggcaggcagg caatagacag ttctccctgg cgaaactcgc gcagactttc
     3121 tgcggcgctg acaatgcgat ccagtccgta ccaggatcgt tgcacttctt caaacagacg
     3181 cagtccttgc acggtaggat gcaatcgtcc acgtacgcgc tcaaacaatt tcagcccgat
     3241 caccttctca aagcgcgcca gttcgcggct gacagttggc tgtgaggtgt gtagcaggtg
     3301 tgccgcctca gtcaggcttc cggcggtcat taccgcatga aaaatttcaa tatgacgtaa
     3361 gttaacggcg gccattagcg ctctctcgca atccggtaat ccatatcatt tttgcataga
     3421 ctcgacataa atcgatattt tttattcttt ttatgatgtg gcgtaatcat aaaaaagcac
     3481 ttatctggag tttgttatgc cacattcact gttcagcact gataccgatc tcaccgccga
     3541 aaatctgctg cgtttgcccg cagaatttgg ctgcccggtg tgggtctacg atgcgcaaat
     3601 tattcgtcgg cagattgcag cgctgaaaca gtttgatgtg gtgcgcttcg cacagaaagc
     3661 ctgttccaat attcatattt tgcgcttaat gcgtgagcag ggcgtgaaag tggattccgt
     3721 ctcgttaggc gaaatagagc gtgcactggc ggcgggttac aatccgcaaa cgcaccctga
     3781 tgatattgtt tttacggcag atgttatcga tcaggcgacg cttgaacgcg tcagtgaatt
     3841 gcaaattccg gtgaatgcgg gttctgttga tatgctcgac caactgggtc aggtttcgcc
     3901 agggcatcgg gtatggctgc gtgttaatcc ggggtttggt cacgggcata gccaaaaaac
     3961 caataccggt ggcgaaaaca gcaagcacgg tatctggtac accgatctgc ccgccgcact
     4021 ggacgtgata caacgtcatc atctacagct ggtcggcatt cacatgcaca ttggttctgg
     4081 cgtcgattat gcccatctgg aacaggtatg tggtgctatg gtgcgtcagg tcctcgaatt
     4141 cggtcaggat ttacaggcta tttctgcggg cggtgggctt tctgttcctt atcaacaggg
     4201 tgaagaggcg gttgataccg agcattatta tggtctgtgg aatgccgcgc gtcagcaaat
     4261 cgcccgccat ttgggccacc ctgtgaaact ggaaattgaa ccgggtcgct ttctggtagc
     4321 gcagtctggc gtattaatta ctcaagtgcg gagcgtcaaa caaatgggta gccgccactt
     4381 tgtgctggtt gatgccgggt tcaacgatct gatgcgcccg gcaatgtacg gtagttacca
     4441 ccatatcagt gccctggcag ctgatggtcg ttctctggaa cacgcaccaa cggtggaaac
     4501 cgtcgtcgcc gggccgttat gtgaatcggg cgatgtcttt acccagcagg aagggggaaa
     4561 tgtcgaaacc cgcgccttgc cggaagtgaa ggcaggtgat tatctggtac tgcatgatac
     4621 aggggcatat ggcgcatcaa tgtcatccaa ctacaacagc cgtccgctgt taccagaagt
     4681 tctgtttgat aatggtcagg cgcggttgat tcgccgtcgc cagaccattg aagaattact
     4741 ggcgctggaa ttgctttaac tgcggttagt cgctggttgc atgatgactt gcctccagcg
     4801 acggagttga cactgaatgg cgacgtacca gcgtcggact aaagacatta gtgatttccg
     4861 ggagagggcg attatccgcc agcgccaaag ccagttcggc agcctgggtc gccatcgtca
     4921 cgattgggta acgcacggtg gtcagacgcg ggcgcacata gcgtgacacc agcacatcat
     4981 caaagccaat taacgaaatc tcacccggta catcaatacc attatcattg agcacgccca
     5041 tcgcgcccgc cgccattgaa tcgttataac aggctaccgc agtgaaattt ctgcctcgtc
     5101 ccaaaagctc ggtcattgcc tgttcgccgc cgctttcgtc tggttcgcca aatgtcacca
     5161 gccggtcatt ggccggaata ccactttcag caagggcatc gtaataccct tgcagacgat
     5221 cttcggcgtc agaaatagag tggttagagc acagataacc aatgcgggta tgaccttgct
     5281 gaattaaatg acgcgtagcc agccaggcac cgtaacgatc gtccagagca atacaacggt
     5341 tttcaaagcc aggcaggata cggttgatca gcaccatacc gggcatttgt ttcattaatg
     5401 aggctaaatc ggcatccggg atcattttgg catggacgac caacgcagca cagcgatggc
     5461 ggatcagttg ctcaatggcc tgacgctctt tttgttcgtt gtggtaaccg ttgccaatca
     5521 ataaaaaatt accagtgtga taagccacct gttcgaccgc tttcaccatt gcaccgaaaa
     5581 acggatcgga aacatcacca acgaccagac cgaccgtttc agtggtctgc tgcgccagcg
     5641 cacgggcgtt ggcgttcggg tgatagctaa gagactccat tgcactatgc acagcaagcc
     5701 gggaagcttc gctggctttg ggtgaattat taatgacgcg ggaaacggtg gcgactgaaa
     5761 cgcctgccag tcgggctaca tcctttatgg tcgccatgaa aataccttag tgggtaaacg
     5821 cttacattct ttcagtgtta cggaaaacgc ccgttggttc aaggggcggc gtgttttcat
     5881 tgtgggaatg tgagccaaac gcaaataagt aagacaaaaa aagagattgc aaacctttgg
     5941 ttacactttg cgaaacgctg ttgcgattga ccgctggtgg cgtttggctt caggttgcta
     6001 aagtggtgat cccagaggta ttgataggtg aagtcaactt cgggttgagc acatgaatta
     6061 caccagcctg cgcagatgcg cagg
//
LOCUS       DAZGHF010000209         6037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGHF010000209 DAZGHF010000000
VERSION     DAZGHF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.210"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            175..822
                     /gene="fucA"
                     /locus_tag="ACHK3A_004668"
     CDS             175..822
                     /gene="fucA"
                     /locus_tag="ACHK3A_004668"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO8742647.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            850..1998
                     /gene="fucO"
                     /locus_tag="ACHK3A_004669"
     CDS             850..1998
                     /gene="fucO"
                     /locus_tag="ACHK3A_004669"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO8742648.1"
                     /translation="MANRMILNETAWFGRGAVGSLTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPTLAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2053..2808)
                     /gene="xni"
                     /locus_tag="ACHK3A_004670"
     CDS             complement(2053..2808)
                     /gene="xni"
                     /locus_tag="ACHK3A_004670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO8742649.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVVGIGPKSATQLLVEFKSLEGIYENLNAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2919..4286)
                     /gene="sdaB"
                     /locus_tag="ACHK3A_004671"
     CDS             complement(2919..4286)
                     /gene="sdaB"
                     /locus_tag="ACHK3A_004671"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO8742650.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4344..5633)
                     /gene="sdaC"
                     /locus_tag="ACHK3A_004672"
     CDS             complement(4344..5633)
                     /gene="sdaC"
                     /locus_tag="ACHK3A_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO8742651.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1531 a         1486 c         1608 g         1412 t
ORIGIN      
        1 aaaaagagaa cttgcagtta tttactgtga ttagttgaac caggtcacaa aatataagct
       61 tgtgaatttc tttctggtta atcaggaaga agatggtggg taattaaacg gctaattcaa
      121 tagtgtgaaa ggaacaacat tattgccctg ttttgaatca gagagaggta gctaatggaa
      181 cgaaataaac ttgctcgtca gattattgac acttgcctgg aaatgacccg cctgggactg
      241 aaccagggga cagcgggtaa cgtcagtgta cgttatcagg atgggatgct gattacgcct
      301 acaggcattc catatgaaaa actgacggag tcgcatattg tctttattga tggcaacggc
      361 aaacatgagg aaggaaaact cccctcaagc gagtggcgtt tccatatggc ggcctatcaa
      421 agcagaccgg atgccaacgc ggttgttcac aatcatgccg ttcattgtac ggcagtttcc
      481 attcttaacc gaccgatccc cgcgattcac tacatgattg cggcggctgg cggtaattct
      541 attccttgcg cgccttatgc gacctttgga acacgcgaac tttctgaaca tgttgcgctg
      601 gctctcaaaa atcgtaaggc aactttgtta caacatcatg ggcttatcgc ttgtgaggcg
      661 aatctggaga aagcgttatg gctggcgcat gaagttgaag tgctggcaca actttacctg
      721 acgaccctgg cgattacgga cccggtgcca gtgctgagcg atgaagagat tgccgtagtg
      781 ctggagaaat tcaaaaccta cgggttacgg attgaagagt aatttcgtaa agcaacaagg
      841 agaaggatga tggctaacag aatgattctg aacgaaacgg catggtttgg tcggggggct
      901 gttgggtctt taaccgatga ggtgaaacgc cgtggttatc agaaggcgct gatcgtcacc
      961 gataaaacgc tggtgcaatg cggcgtggtg gcaaaagtga ccgataagat ggatgctgca
     1021 gggctggcat gggcgattta cgacggcgta gtgccgaacc caacaattac tgtcgtcaaa
     1081 gaagggctcg gtgtattcca gaatagcggc gcggattacc tgatcgctat tggtggcggt
     1141 tctccacagg atacttgtaa agcgattggc attatcagca acaacccgga gtttgccgat
     1201 gtgcgtagcc tggaagggct ttccccgacc aataaaccca gtgtaccgat tctggcaatc
     1261 cccaccacag caggtactgc ggcagaagtg accattaact acgtgatcac tgacgaagaa
     1321 aaacggcgca agtttgtttg cgttgatccg catgatatcc cgcaggtggc gtttattgac
     1381 gctgacatga tggatggtat gcctccagcg ctgaaagctg cgacgggtgt cgatgcgctc
     1441 actcatgcta ttgaggggta tattacccgt ggcgcgtggg cgctaaccga tgcgctgcac
     1501 attaaagcga ttgaaatcat tgctggggcg ctgcgaggat cggttgctgg tgataaggat
     1561 gccggagaag aaatggcgct cgggcagtat gttgcgggta tgggattctc gaatgttggg
     1621 ttagggttgg tgcatggtat ggcgcatcca ctgggcgcgt tttacaacac gccacacggt
     1681 gttgcgaacg ccatcctgtt accgcatgtt atgcgttata acgctgactt taccggtgag
     1741 aagtaccgcg atatcgcacg cgttatgggc gtgaaagtgg aaggtatgag cctggaagag
     1801 gcgcgtaatg ccgctgttga agcggtgttt gctctcaacc gtgatgtcgg tattccgcca
     1861 catttacgtg atgttggtgt acgtaaggaa gatattccga cactggcgca ggcggcactg
     1921 gatgatgttt gtaccggtgg caacccgcgt gaagcaacgc ttgaggatat tgtagagctt
     1981 taccataccg cctggtaaat gcgctgatgt gataatgccg gatgcgacgt ttgcgccgta
     2041 tccggcacgc cgttacctta ccaaccgcaa ttgctgtaaa ttgccgtcga tatgcaaatc
     2101 ggtttgtaag cgggcaatat cgcggcacag aaacgccatc tctttatggg tttctaattt
     2161 tttgcgccac ttttcggcaa ccgcattcag attctcatat atcccttcca gactcttaaa
     2221 ctcgaccagc agctgcgtgg cgctttttgg tccgatcccc acaacacccg gtacctttga
     2281 actgctgatc cccgcaagtc cccagtaatc gggcaactgc tgcggttgaa cgccaaattc
     2341 tttatcgata aatggcgcat ccagccaacg tttctggaag taatcacgaa tacgtaatgt
     2401 cggtgaaagt aactgacagt agcctttatc tgtcgaaaca atcgttgcct gatgcccggc
     2461 ctgtgtcact ttgaccgcca gcgtggcggc taagtcatcg gcttcgttgc cgctggcgga
     2521 ccagcacggg acgccgcgtt gctcaaaggc ggcgcgtaat gcaggcatct cgtcgtgcaa
     2581 ctcttccggc attggcggtc gacccgcttt gtaatctggt aaacgctgat gacgccagcc
     2641 gctactgcgg ttttcatcat caaaaacggc gaccgcgtgg gttggctggc tgtgcataat
     2701 gagctgatcg agcgcgtgct ggcaggtttc gacacagggc gaaccctgaa cggcatgaat
     2761 gcgacgaata agattcagtg catcgacaat aagcaaatga acagccacga taaccccctt
     2821 aaagatcagg cgataagggt aacattcgat gccggaacag gctatggctg gttgagaaaa
     2881 cgggaagagg cctcgcaaaa cgaggcctcc gggagagatt aatcgcaggc aacgatcttc
     2941 attgccaggc caccgcgaga ggtttcgcgg tacttggcgt tcatatcttt acctgtttcg
     3001 tacatggtct cgataacttt atcgaggcag acgcgcggct cgctggtacg gcgcagcgcc
     3061 atacgcgcgg cgttcaccgc ttttactgcc gcaatggcgt tacgctcgat gcacggtacc
     3121 tgcacctgtc cagcaaccgg gtcacacgtc agaccgaggt tatgctccat ggcgatttcc
     3181 gccgcgatgc acacctgcgc cgggcttgcg cctaacagtt ctgccagacc agctgccgcc
     3241 atagagcacg ccacgccaac ttcaccctgg caacccactt cggcaccaga aatcgacgcg
     3301 ttcatcttat aaagagaacc aatggcgctg gctaccagca gataacgagc cagtgagtta
     3361 gcgttcactt cgcggataaa cttatcgtag tacgccagca cagccgggat aatcccgcac
     3421 gcaccgttag tcggcgcagt caccacgcga ccgccagcag cattctcttc gttcactgcc
     3481 agtgcaaaca tgttgatcca gtcaacaacc gccatcgggt cagtggtggt tttatcctgg
     3541 ctgaccagca tccggcgtag tgccgcagca cggcgtggaa cgcgcagttt gccaggtaac
     3601 acgccttcgg tagaaatacc acgctcaata ccgccacgca tgacttccca gacgttcgcc
     3661 aggtgctgtt ccagctcttc tttgctgtgc agcgccagct cgtttttcat catcaggcca
     3721 gagagtgaca gcccggtttc ctgacaatgt ttttgcagat cggctgctga actgtacgga
     3781 taaggaactt caaccggtgc gctatcctgc tggccaaaat gttcttcatc aacgataaag
     3841 ccaccgccga tagagtagta agtctggctg taaacgactt tatcgcccgc cagtgcggta
     3901 atgcgcatac cgttttcatg cagagaaagg ttgtcggcat ggaagttcat gcactgatca
     3961 accgggaact ccacttcatg ctgaccgttt gccagcatca ggcgaccatg agtattcaca
     4021 tcctgaataa aaccagggat ggaatcgata tccacggtat ccggcaggtt acccgccagg
     4081 cccataataa tggcgatatc agtgtggtgg cctttaccgg tcagagagag cgagccgtac
     4141 acgtcaacca ccacgcgggt cacgtcttta agcaggttac gggcaatcag atcgtcggta
     4201 aattgtttac ccgctttcat tggtccaacg gtatgagaac tggaagggcc aatgccgatt
     4261 ttgaaaatat cgaatacgct aatcatagga aatacatcgc gttaaaacgg aggaagcgcc
     4321 gcccgaaagc ggcgcgaaag gacttagctg aacagagagt agaagattgc ggagattgca
     4381 atcagaccca tcacgacaac gaatacgttg ctgatgtgac cgctgtactt acgcattgcc
     4441 ggtactttct gaattgcgta catcggcatc aggaacagga tcatcgcgat gattgggccg
     4501 cccagggttt caatcatacc caggatgctc gggttcaggg tggcaacaat ccaggtcgtt
     4561 accagcatga acagcgcagt gatacggttc agcttgttga tttcgataga cttaccttta
     4621 ccacgcagag atttaatcac cataccgttg aagccttcac gtgcgcccag gtagtggccg
     4681 aggaaggatt tggtgatagc gataatcgcg ataatcggag ccatccacgc gatgatcggc
     4741 gcgttaaagt ggttagccag gtaagacaga atcgagatgt tctgctcttt agccgcagcc
     4801 aggtctgctg gagtcaggct cagtacacag ctgaagacga agaacattac ggtcagcacc
     4861 atcatgatgt gtgcgaatgc caggatcttc gagcatttct gttctgccat atcgccgtac
     4921 tcttcacgct tcgcaacggc gaaagaagag atgatcggag agtggttgaa cgagaacacc
     4981 attaccggaa ttgccagcca cagggtcatc caaagaccgt ttccggttgc agatgcagtg
     5041 tccagagaca gcgtttccag tgctgcgccg ttccactgcg ggatcaggta cagagccagc
     5101 agcatcagta cgccaacaaa cgggaatacc agaatactca tcgctttaac gatcatctgc
     5161 tcaccgaagc gaacgatggt catcataccc acgatcagga tcagcgacag aatcgcacgc
     5221 ggcggtggtg tcatacccag ctggtgagac atgaagcttt caactgtatt ggtgattgcc
     5281 acgctataaa ccagcagaat cgggtagata gcgaagaagt agagcagggt aatcagttta
     5341 cctgcgccaa taccaaagtg ttcttctaca acctcggtga tgtcttcgcc cgggttttta
     5401 ccagacagta cgaagcgagt cagaccgcgg tgagcaaaaa atgtcatcgg gaacgcaagg
     5461 atagccatga tgatcagcgg gatcataccg ccaacaccgg cgttgattgg caggaacagc
     5521 acgcccgcgc cgattgccgt gccgtaaagg cccagcatcc acatggtgtc tgtcttgcgc
     5581 caggcactac gagagtcttt cgacgcaatc gtgctggttt gagtcgtttc catctatttc
     5641 tcctggagga atgcaaataa ttcagctttt agccagttca gcccgggaag gtgcctgacc
     5701 gtaaaaatga aatctgtcta taggcgatgg cttaatcatt taccgccgta ataatttgag
     5761 ggcggaaaga tacacacaag ttatgtatct atcagtgatc ttgatcccaa atacaataat
     5821 ctacttggaa gatagattat cccaacgctt tactctgtaa atcaaaagta aaagagaatt
     5881 taagggcaat atttgcccat taaacgacat ctgacttctt ccggggcgcg caatatatgt
     5941 aggttgaact gaaaaaatca atacttttag ttaatcatgg cgataatttc tttttcatag
     6001 cgataaaatg tggtttatga aaatagggaa acgtttg
//
LOCUS       DAZGHF010000210         6019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGHF010000210 DAZGHF010000000
VERSION     DAZGHF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.211"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(36..1376)
                     /gene="gntU"
                     /locus_tag="ACHK3A_004673"
     CDS             complement(36..1376)
                     /gene="gntU"
                     /locus_tag="ACHK3A_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO8742652.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(1380..1907)
                     /gene="gntK"
                     /locus_tag="ACHK3A_004674"
     CDS             complement(1380..1907)
                     /gene="gntK"
                     /locus_tag="ACHK3A_004674"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO8742653.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(2046..3041)
                     /gene="gntR"
                     /locus_tag="ACHK3A_004675"
     CDS             complement(2046..3041)
                     /gene="gntR"
                     /locus_tag="ACHK3A_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO8742654.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(3265..3960)
                     /gene="yhhW"
                     /locus_tag="ACHK3A_004676"
     CDS             complement(3265..3960)
                     /gene="yhhW"
                     /locus_tag="ACHK3A_004676"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO8742655.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(4083..5120)
                     /locus_tag="ACHK3A_004677"
     CDS             complement(4083..5120)
                     /locus_tag="ACHK3A_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO8742656.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            5454..5942
                     /gene="yhhY"
                     /locus_tag="ACHK3A_004678"
     CDS             5454..5942
                     /gene="yhhY"
                     /locus_tag="ACHK3A_004678"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO8742657.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         1524 a         1624 c         1488 g         1383 t
ORIGIN      
        1 agcccggtag cgtcagaact accgggtaaa caaacttaac tcaacagctg gaacgcaatc
       61 atcccaacga tggcaccgac agtgccgagg atggtttcca tcatggtcca ggttttcagc
      121 gtttcggctt cggtcgcgcc ggtaaattta ccgaacaacc agaaaccggc gtcgttaacg
      181 tggctgacaa caatcgaacc accggcgata caaatcgaca gcgccgccat ttgcgcacca
      241 gagtagttca gttgttcaat gactggcatc accagtccca ccgccgttaa acaggctacg
      301 gtggcagacc cctgaatgat gcgcactgca gctgccagca cgaagcaggt gatagcaatc
      361 ggcaggccca tgccggttaa cgcttcgccc agtgccggac ctacgccaga gtcaaccagc
      421 acctgtttga acacgccgcc cgcaccaatc accagcagaa tgatccccgc cggttgcagc
      481 gcgtgaccgc aaatctccat cactttgtct tttggcatgc cctgacgcat tgccaggcca
      541 taaatcgcta ccagacaagc aaccagaatc gcggtaaacg gatgaccaat aaactcgaac
      601 cattcgtaag cggtagagcc ttctggcaca aaacgcgcgg caatggtttt cagccccacc
      661 agcaccagcg gcaacaggat cagcgacagg ctgaatccga aagatggcat tttaccttcg
      721 ccgagatgcg gttcgctgat gtcgtcagga atatgcagct caacgtaacg gctgatgaag
      781 ttaccccaca gcggcccggc aataatcatt cccggaattg ccgcacacag gccaatcagg
      841 atcatccagc caaagtcggc gttcatctgc gatgccagca gcattggcgc tggccccggc
      901 accaggaacg cagccgctgc cgccacgcct gcaaataatg ggattaccag cttcaccagg
      961 ttcgtaccgg tatgacgcgc cattgagaaa gcaacgctaa tcagcagaac aatcgccact
     1021 tcaaagaaca gcggtagcgc acaaaccagc cccgcaaggc caatggcata atgcgcgcgg
     1081 ctgtgaccga aggatttaag cattttgacg gcaatctgat cgactgcgcc ggtttcatgt
     1141 aagatcttgc caaacatagc tcccagggcg acaaccaccg ccaggaagcc gagggtgcct
     1201 cccatccctt tttccatcgt cgctgcgatt ttatcgagcg gcataccgga aaaaaggcca
     1261 gcccccatgg acaccaccat taaagccagg aaagcgtgca tacgcgcctt catgactaaa
     1321 aacagcagca gtaaaacaga ccctactgct gttaaaacaa gcgttaatgt agtcactact
     1381 tatttgcctt ttttaataac ctcaatggtg cttgccacaa caccttccag cggttgatcg
     1441 atatccacca ccagtacatc ggtttcgtcc gcacccggct cctgcagcgt ttcaaactgc
     1501 gtcaccaaca tttgggtttt aaagaaatgg cctttgcgcg ctttcaggcg gctttcaatc
     1561 acatcaaaat cacctttcag gtagatgaaa gagagattcg gattaccttc acgcagcaag
     1621 tcgcgatagt gttttttcaa tgcagaacag acgatcagtg acactttatt agtgcgctgc
     1681 atagcaaacg cggcgtcgtt cagcgcctgc agccacggtt tgcgatcgtc atcattcagc
     1741 ggttcgccag acgccatttt ttcgatattg cgccgtggat ggaggaaatc gccatcaaga
     1801 aacgcggcat gaagttgatg cgccacttca cttgcgaccg cagatttgcc gctgcccgat
     1861 acgcccatca agacgtaaat gtggtgatca tggttagtcg tgctcaaagt ggtgccccca
     1921 caatacaaga attaaaaatg ttacgggtaa ctgttatcgg taacattgtc cagccggaca
     1981 atatcagaag caatatccat ccgtgcctta agtgtataag tgtgagctac ttcaaatttg
     2041 tgggcttaaa tagatccgcc cggtgacaag gtgaaaccta aatctaacat tttcggtgtc
     2101 acagattcgc cacgaatacg cgccagcagg cgttcagcgc caatactgcc catccgctca
     2161 cgcggcgtca ggacgctcgc caaccgtggc tccatcacct ggccaatgtc atgaccgtgg
     2221 aaaccggcaa tcgccatatc gtcaggaact ttcaacccca gacgctgaca ttcaaacgcc
     2281 gcgccgaccg ccaggtcatc attagtacag aacacgccat ccagctgcgg atattcccgc
     2341 cgtgcctggc gaatcagttc aataccggaa gaataagaag aagattgctc aaccatcacg
     2401 ctatacggca ccaggcctgc atccagcatc gcctgttcat atcccttctg tttgatgata
     2461 gtacgttcgt cgagacgtgc gccgagatag gcaatgtggc gatgcccgcg agcaataatg
     2521 gctgtggtca tctggcgtgc cgcttcaaag ttatcaaaac cgacggcgat atcgaggcaa
     2581 ggcgacttgc tgtccatcag ttccaccacc gggatccccg ccacttcaat catctttaag
     2641 gtgcgcggcg tgtgggtacg ttcggtgagg atcagtccgt cgatattcca tgagagcatg
     2701 gattcgagac gttcttgctc catttccggt ttataaccgt agtgggccag catggtctga
     2761 taaccgtgcg cgtcggtgac gctttcgatt ccgcgtaata cttccgcgaa aacctggttg
     2821 gtgagagaag gtaacaggac gccaatcgcc cggctggtgg cgttagagag gatatcgggt
     2881 gcgcgattgg gaatataacc cagttcatca agagcggcgg caatcttgcc gcgtagagcg
     2941 acggaaacct gctccgggtt acgcaaaaag cggctgaccg tcattttggt cacgcctaca
     3001 cggtcagcca catcctgaag tacgggtctt ttctttttca tcgtcctgaa ggtacaaaag
     3061 agatagattc tcttagttta acacggacgc atcagaacct tcccggggaa aacagtgctt
     3121 gtttacaatt attagatata cactaaatca attgcatcgc attgtgctaa cgacgcggca
     3181 aaaccccgga agcgtacatc agcacgcgac cggagcgagc gaggaagcct gcaaaaaggc
     3241 aggcttcaaa gatgccgttg agctttaaac cggcggcaga tcgaacagta acacttcgct
     3301 gtcgctatcc gcatggatgg agattgcctg ctcatcccag attgccagac catcgctgat
     3361 cgaggctttc acgccgttaa tggtgacatt gcctttcacc acctggatcc agacgcggcg
     3421 ttcagcggca atctgatgca ccgactgctc atctttcagc aacgcccagc ggtacagttc
     3481 catatcctga tgcactttca atgaaccatc acgcgcatct ggcgaaagca ccagttgttt
     3541 gccctgcact gcatcgaagc gacgctgttc ataacgcggc gtaatgccgt tttcttccgg
     3601 catgatccag atctgataca ggtgcagacg ctcggtgctg cttgggttgt actctgagtg
     3661 acgaataccc gtacccgcac tcataatctg gaactcgccc gccggaacct gctctttatt
     3721 gcccatgctg tcctggtgct caacagtacc ttccagcacg taggtcaaaa tttccatatc
     3781 tttatgcggg tgagtgccga agccctgccc tgcttcaatc acgtcgtcgt taatcacgcg
     3841 cagcgcggag aagcccataa agttcggatc gtaatagttg gcaaaagaga aagtatgcca
     3901 ggagtccagc cagccatgat ttgcatgacc acgttcattt gctttgcgta agtagatcat
     3961 ttgtattcgc cccctgaatg atttcgatgg gcttagtgtg gacccgatct gcctgggatg
     4021 atagagggtg aaaattgacc cctctgttca aaaaaattga actattcgag gggccatcaa
     4081 acttacttcg cgagagtcac tgtggaggga gaggcttgct caaatccgcg ttcaaggatt
     4141 tccagattgg taagaacttc agattccttg acgtaatttg gcgcaccgtt ggtgatggtt
     4201 tgatacaacg catcataaac gcgcccgtaa tcgcccatct ccggcttcat ctcttctctg
     4261 accgtcacac cctcgtcatt gacatactcc agcacaccga ccgaatcatc cgctgcgaat
     4321 cccggttcgc ccggcataat attagccttg aggctggttt cctgctggtc gataccgtat
     4381 ttaataaacg aacctttctt accgtgaacg ataaatttcg gataatcgat tttcaccaga
     4441 tggctggttt tgacgatggc tttcaagtcg ccataaaaca gttgcgcttc aaaggtgtcg
     4501 tcaggattgg ctttattgcg caggctgcgg atgtcataag cgacgtgatc cgggcgaccg
     4561 aacagagaaa taatctggtc catcgtatgc acaccaaggc catagaacgc gccatcctgc
     4621 ggcaacccag gtttggtttc tgccaccggg cggtaatagt caaaatggct ttccacttca
     4681 acaatctctc ccaacttgcc gctttcaatc gcttttttcg ccgtcaggaa gcaggaatca
     4741 aagcgacgat tctgatacgg cgtgacggtc agccctttgc ttttcgccag cgcaaacagc
     4801 tccttcgcct gggcaagcgt cggggtgaac ggtttttcga ccagcacatt tttcccggct
     4861 tccagcgcgc gtttcgcgta ttcgaaatgg ctgtccgcgt gggtgcagac gacaaccagc
     4921 ttaacatcgg gatcgtttag cacttcgtcg agatcgctgg tgaaatggat atgagagtaa
     4981 atgggagcct gttcttccgg ctttgcatgg cggcgaaaaa tatgcgcgac atgccagcta
     5041 tccttgcggt taagtacata cggcagatgg taacgggtgg tgcttttgcc gaagccaata
     5101 aaggcgcagt tgatgaccat gatttcgtcc tttttaaggt ggttatttac accttagcgc
     5161 aaagcggacg tggttcctac tggagtgcgc ataacgaaca caagcacttc ccgaggataa
     5221 attgagaacg aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
     5281 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
     5341 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
     5401 attcgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
     5461 agatagtgat acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc
     5521 agccggaggt gtattgcaac acactacagg tgcctcatcc ctccgatcat atgtggcagg
     5581 aacgactcgc cgatcgcccc ggcatcaagc aactcgtcgc ctgtattgat ggaaccgtag
     5641 taggtcatct caccattgat gtacagcaac gcccgcgccg cagtcatgtt gccgattttg
     5701 gtatctgtgt cgactcccgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
     5761 tgattgaaat gtgcgacaac tggttgcggg tagatcgtat tgagctaacg gtgttcgtcg
     5821 ataacgcgcc agcaattaag gtctataaaa aatttggctt tgagattgaa gggactggca
     5881 agaagtacgc attacgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaagt
     5941 aagatagtgc cctttttctg agatggaaaa agggtgtcat tcaaaatcgg cataccttcc
     6001 tttaaggtat ttattttcc
//
LOCUS       DAZGHF010000211         5992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGHF010000211 DAZGHF010000000
VERSION     DAZGHF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.212"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            228..509
                     /gene="yjcB"
                     /locus_tag="ACHK3A_004679"
     CDS             228..509
                     /gene="yjcB"
                     /locus_tag="ACHK3A_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO8742658.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(609..1145)
                     /gene="ssb1"
                     /locus_tag="ACHK3A_004680"
     CDS             complement(609..1145)
                     /gene="ssb1"
                     /locus_tag="ACHK3A_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO8742659.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            1400..4222
                     /gene="uvrA"
                     /locus_tag="ACHK3A_004681"
     CDS             1400..4222
                     /gene="uvrA"
                     /locus_tag="ACHK3A_004681"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO8742660.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(4257..4613)
                     /gene="yjbR"
                     /locus_tag="ACHK3A_004682"
     CDS             complement(4257..4613)
                     /gene="yjbR"
                     /locus_tag="ACHK3A_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO8742661.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4617..5033)
                     /gene="yjbQ"
                     /locus_tag="ACHK3A_004683"
     CDS             complement(4617..5033)
                     /gene="yjbQ"
                     /locus_tag="ACHK3A_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO8742662.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(5144..5857)
                     /gene="aphA"
                     /locus_tag="ACHK3A_004684"
     CDS             complement(5144..5857)
                     /gene="aphA"
                     /locus_tag="ACHK3A_004684"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO8742663.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1424 a         1579 c         1564 g         1425 t
ORIGIN      
        1 atgcccttaa tgccactgaa aaaccacata atctgtacag caataaaagt cacggcctaa
       61 tctggctaac aaacaatcaa ttgtgatata gttcacaaaa ttaatgaaac aaacagagtg
      121 tttcattttt gtgtcctgca agccccgcga tagccagagt gtaccacgcc tcccgtgaac
      181 aacgccgcgc tgtccagggc atcggctctt ttcagaggaa ttgtttgatg gctaccctca
      241 ccactggcgt ggttctactt cgctggcaac ttcttagtgc cgtaatgatg tttctggcca
      301 gcacgctcaa catccgtttt cgtcggtctg attatgtcgg gcttgcagtg atcagcagcg
      361 gtctgggcgt ggtttctgcc tgctggttcg caatggggtt gcttggcatc acaatggcgg
      421 atatcaccgc catctggcac aacatcgagt cggtgatgat tgaagagatg aatcagacac
      481 cgccacaatg gccaatgatt ttaacttgat atgtagaagc ctccataatg gaggcttctt
      541 tttttatggc tggcagatgc tttaatcatc caccttaaaa caatataacc tattgtttta
      601 atgacaaatc agaacggaat atcgtcatca aaatccatcg gcggctcgtt agacggtgct
      661 gccggagcgg actgctgcgg gcgagactgc gcgccgccgc tgaactgatt gccgccctgc
      721 ggctgctgag gctgacccca accgccctgc ggctgaccac caccgatatt gccacctgca
      781 ggagcgccac caccctgacg accacccagc atctgcatgg tgccgccaac gttcaccacg
      841 acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc atttgcgggt acgcagctga
      901 ccttcgatat aaacctgaga acctttacgc agatattcgc tggctacttc tgccagtttg
      961 ccgaacagca caacgcggtg ccactcagtc tgctctttca tctcgccggt cgctttgtca
     1021 cgccaggatt cggaagtagc cagcgtaatg ttggcaacag cgccaccatt tggcatgtag
     1081 cgtacttccg ggtcctgacc cagattacca acgagaataa ccttgtttac gcctctgctg
     1141 gccatgttcg tgtctcctga aaaaaaatcg ttctgaataa gtgtaaacgc gcgattgtac
     1201 cattaccaat agcgctttta ctatgttgtg acctcggttc cgggaaacaa acctggccag
     1261 acattgttac acaacactcc gggtaatgca ttccaatact gtatattcat tcaggtcaat
     1321 ttgtgtcata attaaccgtt tgtgatcgcc ggtagcacca tgccaccggg caaaaaagcg
     1381 tttaatccgg gaaaggtgaa tggataagat cgaagttcgg ggcgcccgca cccataatct
     1441 caaaaacatc aacctcgtta tcccccgcga caagctcatt gtcgtgaccg ggctttcggg
     1501 ttctggcaaa tcctcgctcg ctttcgacac cttatatgcc gaagggcagc gccgttacgt
     1561 tgaatccctt tccgcctacg cgcggcagtt tctgtcactg atggagaagc cggacgtcga
     1621 ccatattgag gggctttctc ctgccatctc aattgagcag aaatcgacgt ctcataaccc
     1681 gcgctctacg gtggggacaa tcaccgaaat ccacgactat ttgcgtctgc tgtacgctcg
     1741 cgttggtgag ccgcgctgcc cggaccacga tgttccgctg gcggcgcaaa ccgtcagcca
     1801 gatggtggat aacgtgcttt cgcagccgga aggcaagcgt ctgatgctgc tcgcgccaat
     1861 cattaaagag cgcaaaggcg aacacaccaa aacgctggaa aatctggcaa gccagggtta
     1921 catccgtgct cgtattgatg gcgaagtctg cgatctttcc gatccgccga aactggaact
     1981 gcaaaagaaa cataccattg aagtggtggt tgatcgcttc aaggtgcgtg acgatctcac
     2041 ccaacgtctt gccgagtcgt ttgaaaccgc gctggagctt tccggtggta ccgcggtagt
     2101 tgccgatatg gacgatccaa aagcggaaga gctgctattt tccgccaact tcgcctgccc
     2161 aatttgcggc tacagtatgc gtgaactgga accacgcctg ttttcgttta acaacccggc
     2221 gggtgcctgc ccgacctgtg acggccttgg cgtacagcaa tatttcgatc ctgaccgcgt
     2281 gatccagaat ccggaactgt cgctggcagg tggtgcgatc cgaggctggg atcgccgcaa
     2341 cttctattac ttccagatgc tgaaatcgct ggcagatcac tataagttcg acgtcgaagc
     2401 gccgtggggc agcctgagcg cgaatgtgca taaagtggtg ttgtacggtt ctggcaaaga
     2461 aaatattgaa ttcaaataca tgaacgatcg tggcgatacc tccattcgtc gtcatccgtt
     2521 cgaaggcgtg ctgcacaata tggagcgccg ctataaagag acggaatcca gtgcggtacg
     2581 tgaagaatta gccaagttta tcagcaatcg tccgtgcgcc agctgcgaag ggacgcgtct
     2641 gcgtcgggaa gcgcgccacg tttatgtcga gaatacgccg ctacctgcta tctccgacat
     2701 gagcatcggt catgcgatgg aattcttcaa caatctcaaa ctcgcaggtc agcgggcgaa
     2761 gattgcagaa aaaatcctta aagagatcgg cgatcgtctg aaattcctcg ttaacgtcgg
     2821 cctgaattac ctgacacttt cccgctcggc agagacactt tctggcggtg aagcccagcg
     2881 tatccgtctg gcgagccaga ttggtgcggg cctggttggc gttatgtacg tgctggacga
     2941 gccgtctatc ggcctgcacc agcgcgataa cgagcgcctg ctgggtacgc ttatccatct
     3001 gcgcgatctc ggtaataccg tgattgtggt ggagcacgac gaagacgcga ttcgcgccgc
     3061 tgatcatgtg atcgatatcg gtccgggtgc gggtgtacac ggcggtgaag tcgtcgcgga
     3121 aggtccgctg gaagcaataa tggcggtgcc agagtcgttg accgggcagt acatgagcgg
     3181 taaacgcaag attgaagtgc cgaagaagcg cgttccggca aatccggaaa aagtgctgaa
     3241 actgacaggc gcacgcggca acaacctgaa agacgtgacg ctgacgctgc cggtgggtct
     3301 gtttacttgc atcacagggg tttcaggttc cggtaaatcg acactgatta acgacacact
     3361 gttcccgatt gcccaacgcc agttgaatgg tgcgaccatc gccgaaccgg caccgtatcg
     3421 cgatattcag ggactggaac atttcgacaa agtgatcgat atcgaccaaa gcccaattgg
     3481 tcgtacccca cgttccaatc cggcaaccta taccggcgtg tttacacctg tacgcgaact
     3541 gttcgcaggc gtaccagaat cccgtgcgcg tggttatacg ccagggcgtt tcagctttaa
     3601 cgtccgtggc ggacgctgcg aggcctgtca gggcgacggc gtgatcaaag tggagatgca
     3661 cttcctgccg gacatttacg taccgtgcga ccagtgcaaa ggcaaacgct ataaccgtga
     3721 aacgctggag attaagtaca aaggcaaaac catccacgaa gtgctggata tgaccatcga
     3781 agaggcgcgt gagttctttg atgcggtgcc agctctggca cgtaagctgc aaacgctgat
     3841 ggatgttggc ctgacgtaca ttcgcctcgg gcagtccgca accacacttt ctggtggtga
     3901 agcccagcgc gtgaagctgg cgcgtgagct gtcaaaacgc ggcaccgggc agacgctgta
     3961 tattctcgac gagccgacca ccggtctgca cttcgccgat attcagcaac tgctcgacgt
     4021 actgcataaa ctgcgcgatc agggcaatac cattgtggta attgagcaca atctcgacgt
     4081 gatcaaaacc gctgactgga ttgtcgacct gggaccggaa ggcggcagtg gcggcggcga
     4141 aatcctcgtc tccggtacgc cagaaaccgt cgcagagtgc gaagcttcgc atacggcgcg
     4201 cttcctcaag ccgatgctgt aatcgttaag gccgctttca gagcggcctt cttctttcag
     4261 agttgcacca gcaatttacg tttttcttcc ggcagcaaat tcaccgcctg ctgataagac
     4321 gcatccacca gataatagat ttgcgaatcc ggcagcgaac cgtcgagata cacggtgctc
     4381 cagtgcgctt tattcagatg gcggcttgga cgcacatcgc tgtgctgctg acgtagcagc
     4441 tccgccagct ccgggctggt tttcagcgaa acagctgggc gattttctac ttctttcacc
     4501 atcgcaaaca gcacatcttc cactttgatc tgcgtcgctt tccagtcgtt atgcacgctc
     4561 tgttctgcgc ctggttttgc catgcaatat tgtagcaact ccgaaatggt catttttcac
     4621 tccccttgta gtgtcgcgat gatgcgacgc gatccgccgt ggatgcgatg ttcccccagc
     4681 cagatgcctt gccaggtgcc ggtctgaata cgccctttat gcaccggcaa tacaagcgat
     4741 gttcccagca ttgaggattt gatatgagaa ggcatatcgt ctgctccctc atagtcatgc
     4801 tcataatttc cgttatcggg aacggcgcgg aggaaaaaac gctccatgtc gtggcgtacg
     4861 gtgggatcgc agttctcatt aagtgtcaga gaggcggagg tatgttgcag caacagatgc
     4921 agtaaaccga tgttaacgcg cggcatatca gccagctgat tcagaatttc atccgttacc
     4981 agatgaaacc cacgagattt ggcgctaagc gtgagcgtct tttgatacca catgtgctgc
     5041 tccttgataa aactctctta atcagtttgc agcaagacag cgaaaggata aaggtgtgat
     5101 taaaaaaaca gcattcggga gagtattacg ctctcccgct ctgtcagtat tctgaattga
     5161 cgatcacctc ttcaccaaac gcacccgctt gtggcaaggg tttgtaggta gagttggagg
     5221 cgcgcagaat gcggatacca cgagcgccga catcgcgtgc ggcggtaata tcgttatcgg
     5281 aatcaccata aaaaattcgg atatttttat cctgcagcca ttgcgatttt gtattttgcc
     5341 ctggtttatc gcccgcaaag atcaccggat tcatgttggt ggcaggaata tgaaaattat
     5401 ccgccagcgt ttttgaaacc gtttctgttt tggtcgggct acgaccagtc acaaagaaga
     5461 tcgcgtcacc gcggcgtaca tgcatatcaa tcagctggcg agcgacctct tttggaatgc
     5521 tgaattcatc ccagccattg ttcatttttt cccagaacac aggatttttc agataatctt
     5581 cgctttctgg cgagaaggtt tttttgccgc gccagaagcc cggactggaa aaaagtaccg
     5641 tgtcatcgat atcaaacccc accgccattg gcggacgccc tgcgaggcta ttttcaattt
     5701 gtgcgaccga aacccaatga attggtgcct gttcagcaag cctggcaacg ttagtaccag
     5761 ggttaagcgg tgaaggagat gaggccaggg caacagcgga actgtttaga gcgaacaata
     5821 agcaaacggc actgattgcc tgtgtgatct tgcgcatatt tttccctaaa tagtcagtat
     5881 gttgaaactt tttattgtga gatttgttgc aaaaaccatc tgaccataac gccagcagcc
     5941 ggggaaagga aggattatct gcggtttttg tgatgaaaag caggatgatt at
//
LOCUS       DAZGHF010000212         5992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGHF010000212 DAZGHF010000000
VERSION     DAZGHF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.213"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            87..1638
                     /locus_tag="ACHK3A_004685"
                     /pseudo
     CDS             87..1638
                     /locus_tag="ACHK3A_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
     gene            1664..2116
                     /locus_tag="ACHK3A_004686"
     CDS             1664..2116
                     /locus_tag="ACHK3A_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO8742664.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTFIPERLHKT"
     gene            2269..3543
                     /locus_tag="ACHK3A_004687"
     CDS             2269..3543
                     /locus_tag="ACHK3A_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8742665.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3536..5637
                     /gene="mchF"
                     /locus_tag="ACHK3A_004688"
                     /pseudo
     CDS             3536..5637
                     /gene="mchF"
                     /locus_tag="ACHK3A_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
     gene            complement(5698..>5992)
                     /locus_tag="ACHK3A_004689"
     CDS             complement(5698..>5992)
                     /locus_tag="ACHK3A_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742666.1"
                     /translation="LIHGAGLDKDASVAIGTAAGGFLGKATKIPSADIAGSAIGGYIG
                     GVIADGSNRTITIPSNGINYNPSIGLGSFNPNYNGGLLNSGFNSSTSSSSSGS"
BASE COUNT         1720 a         1146 c         1440 g         1686 t
ORIGIN      
        1 ctgtaataag gaagaagctg tcaggacact tacttgctct tttatgaaca ctcttttatg
       61 aaaattgaaa cggagaatac tgccagatga gtcatcagtg ttcactttct gaactgaatg
      121 aaaacctggt gcctttcact gccaggcaga tcaaggcctc attaatctgg tgtgcagagg
      181 atgtcagaaa tccagacgag ctgcaaaatg cctgcagtta tattatcgat cctggcagta
      241 cagcttctgc caaagtgttc catgcagagc gctatggtgg cagtggtatt cagcgtaatg
      301 gaggtggtgc acgttgtggg tttgatggta actaccaggt taaaggaata ggaagtaatc
      361 cgttggttgg cgaaggtact gacgaacgtc attctaatgg tgcactcggc gctgttcatg
      421 caatattatg aggctttgtg gggagaagta ctggctcaaa tattacctta tagtgctgtg
      481 cgggttcggg cggttttact tacagatctc tatactgaaa aggcatttga gcgctccggt
      541 aggaaatcac gaagagccct gttggtacgt gagcctgttg ttcgtccggc gcattttgaa
      601 cgggcacctt acttccaagt aaaaccggag tattccagtc tgctaattca cgatgcctgt
      661 cgggttagat ctgtgatcca caagctgcca ggatatctac ctgtaccacc ggaagaaatt
      721 gatgctgaag cacgaactga tccccggatt tattgcattg aggggttatg tgaactggca
      781 cgtcgtgagg cctggcaaat ggcattttgt cgaacacgtt tcctgagatt gacaacttct
      841 ccttctaata ttgcaatgga tggcagatta atggatttta acggactcag ttgtctgttt
      901 ccgggagatt ccccagctga ttttgggtat aaactaagat tagctgaact ggcaaaagaa
      961 ccgatggtac ttatgcaagg gctgtctgat ctctgcttgt atatcggaaa atatatgttt
     1021 gaccctgact tcactcttgc agcccgtttg aaggttgagg agatatttca gaaaactttc
     1081 catgaagcat gttattactg ttatctagaa ctgttgggta ttcctggaga atttataaca
     1141 caaaaagaga tacctgatat attgaaagaa ctggttaaca gttttgttgc attactcaat
     1201 caatactgcg agaaatcaca tgcccaagat attgtcaatc aggatggttc accattgcaa
     1261 aagttggttg tgacgctaat ccatcatagg cataatcaaa agcaggcact gaatagtagc
     1321 atcaagaatg atgtttattt caccgttgca caacagtgtt tttcccaagc catccactgg
     1381 ctgacgcaag gcagtaccag acgtcagata aatgcctcat tactcctgaa agaaattgaa
     1441 catcatacca tgaaaaggct gcaacccagg gaagagctga ggaaagaaaa tatgtgcgaa
     1501 aaaattgcca tcctgctgga taatcatggc gatgatcccc tttttttaca agaagcaatt
     1561 tctgatatga aaaattttat gcttaagttt tccagagatg catttggata tcttgaaccg
     1621 ataagaaaca cagtgtaaaa aatatataag ggaaatagta atcatgtctt atataaggga
     1681 aaccatcaga ggaaaagatg aatggactgt ttatgaacag atcggttttg cggtcagttg
     1741 tatgctctac aatcgtaatt acagtctgta tccggtgtta accattcaat actggactga
     1801 atatgcgata cagcataatc agattaaatt cctgtttgat tcacgaggtt ttccactggc
     1861 gtatattacc tgggcatatc ttgaggctga tacagaagcg cgcctgctca gggatccaga
     1921 attcaggttg catccgtctg aatggaatga agatggaagg atttggattc tggatttctg
     1981 ttgtaaacca ggctttggtc gaaaagttat tgactatctc atacagcttc agccatgggg
     2041 agaaggagaa gtacgatggt taagcaggcg aaagaaaatt gtgacattca tccctgagcg
     2101 gctgcataaa acgtagtacc tctgaagata cagagataat tgtaaattac ggggtaaatg
     2161 catcgctgat actattttga caggactctg tattttctgg ctttgattat agcatttcgc
     2221 ttaaccagtg aaggtattac aggaaaagtg tgagtaagag gagctatttt gtttcgtcag
     2281 gatgctttag aaaacagaaa aatgaagtgg cagggacggg caatattact tcccggaata
     2341 ccactatggt taatcatgct gggaagcata gtgtttatta cggcatttct gatgttcatt
     2401 attgttggta cctatagccg ccgtgttaat gtcagtggtg aggtcacaac ctggccaaga
     2461 gctgtcaata tatattcagg tgtacaggga tttgttgtca ggcagtttgt tcatgaagga
     2521 cagttgataa aaaaagggga ccctgtttat ctgattgaca tcagtaaaag tacacgtagt
     2581 ggtattgtca ctgataatca tcgtcgggat atagaaaacc agctggttcg tgtggacaac
     2641 attatttccc gtctggaaga aagtaaaaaa ataacgctag ataccctgga aaaacaacgc
     2701 ctgcaataca cagatgcgtt tcgtcgctca tcagatatta tacagcgtgc agaggaaggg
     2761 ataaaaataa tgaaaaacaa tatggagaat tacagaaact atcaggcaaa agggctgatt
     2821 aataaagatc agttaactaa ccaggtggca ttatattatc agcaacaaaa caatcttctc
     2881 agcctgagcg gacagaacga acagaatgct ctgcagataa ccactctgga gagtcagatt
     2941 cagactcagg ctgcagattt tgataaccgt atctaccaga tggaactgca acggtacgag
     3001 ttacagaaag aactggttaa cactgatgtg gagggcgaaa tcattatccg ggcgttgact
     3061 gacgggaaag ttgactccct gagtgtcact gtcgggcaaa tggtcaatac cggagacagc
     3121 cttctgcagg ttattcctga gaacattgaa aactattatc ttattctctg ggtcccgaat
     3181 gatgctgttc cttatatttc ggctggtgac aaagtgaata ttcgttatga agcctttccg
     3241 gcagaaaaat ttgggcagtt ctctgctacg gttaaaacta tatccaggac tcctgcgtca
     3301 acacaggaaa tgttgaccta taagggagca ccacagaata cgccgggcga ctctgttccc
     3361 tggtataaag tcattgcgat gcctgaaaag cagattatca gatatgacga aaaatacctc
     3421 cctctggaaa atggaatgaa agccgaaagt acactatttc tggaaaaaag gcgtatttac
     3481 cagtggatgc tttctccttt ctatgacatg aaacacagtg caacaggacc gctcaatgac
     3541 taacgggaat ttcagacaaa ttataaatca gcttgatatg cgctggcgac gtcgtgttcc
     3601 ggttattcat cagacggaga ccgctgaatg tggactggcc tgcctggcaa tgatatgcgg
     3661 tcattttggt aagaatattg acctgatatc tcttcgccgg aagtttaatc tctcggcccg
     3721 tggagcaaac cttgcaggaa tcaatggaat agcggagcag ctggggatgg tcacccgggc
     3781 tctttcactg gagctggatg aacttggtgc cctcaaaatg ccgtgtattc tccactggga
     3841 tttcagtcac ttcgtcgtgc tggtcagcgt aaagcgtaac cgttatgtac tgcatgatcc
     3901 ggccagaggc agaagatatc tcggtcggga ggaaatgagc cggtatttta cgggcattgc
     3961 acttgaggtc tggcccggaa gtgaattcct ggcggaaacc cagcagaccc gcataagtct
     4021 ccgttcactg attaacagta tttacggtat taaaagaaca ctggcgaaaa ttttctgtct
     4081 gtcagttgta attgaagcaa tcaatctggt aatgccggtg ggaactcagc tggttatgga
     4141 tcatgcgatt ccggcggggg acagagggct gctgacgctt atttctgctg gcctgatgtt
     4201 ctttatattg ctcagggccg cggtgagtat gctgcgtgca tggtcctcac tggttatgag
     4261 cacgctcatc aatatacagt ggcagtcggg tctgtttaac catcttctca gactgccgct
     4321 ggcgtttttt gaacgccgta aattaggtga tatccagtcg cgttttggct cccttgacac
     4381 tttgagggcc acctttacca cctgtgttgt tggggcaatc atggacagta ttatggttgt
     4441 gggtgttttt gtgatgatgc tgttatatgg aggatatctt acctggatag tgctcggttt
     4501 taccatggtt tacgttctta ttcgtctggt gacatacggc tattaccggc aaatatcgga
     4561 agaaactctt gtcagggggc ccgggccagc tcctatttta tggaaagcct gtatggtatt
     4621 gccacggtaa aaatccaggg tatggctggg attcggggaa cacactggct taacctgaaa
     4681 atagatgcga tcaattcagg tattaagtta accaggatgg atttgctctt cggggggata
     4741 aatacttttg ttgccgcctg tgatcaggtg gcgattttat ggctgggtgc aagccttgtg
     4801 atcgataatc agatgacaat aggaatgttt gtggcatttg gttcttttcg tgggcagttt
     4861 tcggatcggg ttgcttcgct gaccagtttt cttcttcaac tgagaataat gagtctgcat
     4921 aatgagctca ttgcagatat tgcactacat gaaaaggaag aaaagaaacc ggaaattgaa
     4981 atcgttgctg acatgagccc ggtttcactg gaaaccactg atttaagcta ccggtatgac
     5041 agccagtcag cacaggtatt cagtggtctg aatttgtctg tggctccggg agaaagtgtg
     5101 gctataactg gtgcctccgg tgccggaaaa accacattaa tgaaagtatt atgtggactg
     5161 tttgaaccag atagtggaaa agtactggtt aatggcacgg atatacgtca acttggaata
     5221 aataattatc accgtatgat agcctgtgtt atgcaggacg accgactatt ttcaggatca
     5281 attcgtgaaa atatctgtgg gtttgcagaa gaaacagacg acgaatggat gacagaatgt
     5341 gccaaagcaa gtcatattca tgatgtgata atgaaaatgc caatggggta tgaaacgtta
     5401 ataggtgaac tgggggaagg tctttccggc ggtcaaaaac agcgtatatt cattgcccga
     5461 gctttatacc ggaaacctgg aatattattt atggatgagg ctacaagttc tcttgataca
     5521 gaaagtgaac gtttcgtgaa tgctgctata aaaaagatga atatcacccg ggtgattatt
     5581 gcacatagag agacaacttt aagaacagtt gatcggattt ttagaattac aaattaggat
     5641 aatatatcac taaaagcagc attgctgcat aataagctat gctgctttag aaattaatca
     5701 acttccacta ctagaagagg atgttgatga gttaaatcct gagtttaata aacctccatt
     5761 gtagtttgga ttaaaacttc caagtcctat actcggatta tagttaatac cgtttgatgg
     5821 tattgttatt gtcctatttg aaccatcagc aataactcct ccaatatatc ctccaattgc
     5881 acttcctgct atatccgcac tagggatttt tgttgcttta cctaaaaaac caccagccgc
     5941 agtacctatt gcaacggatg catctttatc taatcctgca ccatgtatta aa
//
LOCUS       DAZGHF010000213         5912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGHF010000213 DAZGHF010000000
VERSION     DAZGHF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.214"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            185..766
                     /gene="mdaB"
                     /locus_tag="ACHK3A_004690"
     CDS             185..766
                     /gene="mdaB"
                     /locus_tag="ACHK3A_004690"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO8742667.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     ESDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            797..1111
                     /gene="ygiN"
                     /locus_tag="ACHK3A_004691"
     CDS             797..1111
                     /gene="ygiN"
                     /locus_tag="ACHK3A_004691"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO8742668.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1156..2043)
                     /locus_tag="ACHK3A_004692"
     CDS             complement(1156..2043)
                     /locus_tag="ACHK3A_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO8742669.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTMLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2040..2987)
                     /locus_tag="ACHK3A_004693"
     CDS             complement(2040..2987)
                     /locus_tag="ACHK3A_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO8742670.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAGLTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELVADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(2998..4035)
                     /locus_tag="ACHK3A_004694"
     CDS             complement(2998..4035)
                     /locus_tag="ACHK3A_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO8742671.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4044..5027)
                     /locus_tag="ACHK3A_004695"
     CDS             complement(4044..5027)
                     /locus_tag="ACHK3A_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO8742672.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILIFDEQTLLAMRTWLAGDLAGQDWVTLGTSAWFSLGGFVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5024..5833)
                     /locus_tag="ACHK3A_004696"
     CDS             complement(5024..5833)
                     /locus_tag="ACHK3A_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8742673.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         1674 a         1610 c         1363 g         1265 t
ORIGIN      
        1 tgggaactta taggaagttg atttcagtat tgtcattttc agaaaatatt tatcccgcat
       61 cggtgaatca gagcaagatc agacttttgc taaattcgca aaagactttg cacattttgc
      121 taatttcacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac cttgaggtta
      181 aaaaatgagc aacatcctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca
      241 actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt
      301 ccgcatcgtt cgcgccgaga gcgactacga tgtcaaagcc gaagtacaaa actttctctg
      361 ggctgatgtg gtgatctggc agatgcctgg ctggtggatg ggcgcgccgt ggacagtgaa
      421 aaaatacatt gatgatgtat tcaccgaagg tcacgggact ctgtatgcca gcgatggtcg
      481 tacccgcaaa gatccgtcga aaaaatacgg ttctggcggt ctggtacaag gtaaaaaata
      541 tatgctttcc ctgacctgga acgcaccaat ggaagctttc accgaaaaag atcagttctt
      601 ccacggggtt ggcgttgacg gtgtgtatct gccgttccat aaagcaaacc aattccttgg
      661 tatggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg
      721 ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag agctctggct
      781 ttagaaggag ttaaccatga ttacagtaat cgcagaaatc cgtactcgtc ctggtcaaca
      841 tcaccgtcag gcagtgttgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga
      901 aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat
      961 ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcatc ttgaagcgca
     1021 tctgcaaacc ccgcacatga aagcatatag cgaagccgta aaaggtgacg tgctggagat
     1081 gaatattcgt atcctgcagc cggggctttg acatttctaa aaagaggccg gtgctgctac
     1141 cggtctcagc ccagattagc cattctttct tcccgttttc agtcctcgat ggaaatcgtt
     1201 aattcgctta atggttttaa ccgcacgctg cggcactgcg gaagctaccg accagacaat
     1261 catttccaga cacaacaata ccgtaccatg tagcggcgtt ttcccttttt catccccgcg
     1321 cggaacatga atcacgatac tggcgtcttt gctaaaacgt gaatccagcg cgtttgtcag
     1381 caaaataaca gggatcccca gccgcctcgc ctcacgaagc attgtcagcc cttcccggtg
     1441 cgcagatttt tgcgccatca tgatcagaac atcgccacgt tgaagcgcga tcagttgctc
     1501 ggcaaggcca atacccgtac ggtttagtgc cgtggcaggc aaacctatgc ggttgaatag
     1561 tcgtgcggta tagtccgcca gaatacccga agcgccaatg ccaaaaatag cgacttgccg
     1621 tgcctgcacc aacaacgcta cagcttgcgc aagcgcgtgg cggttttcag gctctgacag
     1681 gacattacag gtatacaaat gcccttcaag aacaaagtcg attgcggtgt taacatcgct
     1741 cgtcaagtta ctcaccgtcg tagacatctt ttcgctggaa ctaagggctg gaccaagcca
     1801 ctgttccaga gtgcgtttca gatcacgcag cccggcaaac cccagggcct gtatagccct
     1861 gataactgtg gcatcggaag tgttcaacgt ggccgcaatt tccatcgccg tttgctcgag
     1921 taccacttca cgatgctcat taatgtagct ggcgacaact cttaacccag gcgttaactg
     1981 actcgcgcga gtgcgaaaac gctcaccaaa aatatcaacc cgcactttat ctttctggat
     2041 catttagcct ccggtagagg tcgccctgca atttgtgatt gtatctgcgc agccatcagc
     2101 cccagagatg caaaggagtt agaaattgcc tcttcacgag aaatcagcac ataacgcccg
     2161 gaacgacagg cagtcagaaa ctgacaccat cccggcatga ctttttccat tgctgccagc
     2221 tcatcctgag gtttaccacc ggtgtcaccg cgccaggttg caaaaacaaa atcggcaacc
     2281 agttcaggta aacgctccgc gctcacatcc attcgcccac cttcaggaat gctctcaatc
     2341 agcggcggaa aacgaaaacc cgcatcgcgt aatacccgcc ccagcgaatg atagctatgc
     2401 atcacattaa tttttccctg gtttgcctga atcaccgata ccgttatttt ttggctgtcc
     2461 agcgtcgcct tcagcgcatt gatttgtgcc tgatagcggc gttcaagtat cgccagctgc
     2521 gactgcgttc ctgtaagtcc tgccagcttg cgatagattt ctggcgcacc ccctttgaga
     2581 tgatcaatgc tcaccgtggg cgcaattttt tcaagttgtt caatgggcgt attacgggtc
     2641 ggttctgtga tgatcaggtc aggctttgcc gccacaatcg cttcaatatc aatatcagcg
     2701 gtaccaataa aggcgatcga tgaattatca aagtcgacac ctgtcaataa cgcgccagat
     2761 cgaatgaaat ggctaccgtc aggccgagtt cgcccgtggc tggcgacagg aggtacaccg
     2821 agctcaatca gaggaatagt gatatcaaga tcgtgtaaag agacaattct ctttggatga
     2881 gcaggaacag tgactttgcg ccctaaatca tcagtaaata cctgaacagg ttctgttgca
     2941 cgggaaaaaa atgacaacag tatcagcaga gaaaaaaata aacgcatata actcccgtta
     3001 aaaactgttc ctgcgctgcc agagcagtag caaaaaaaat ggtccaccaa tcagtgagat
     3061 aatgattccg gcaggcagtt gcaaaggcaa aaaagccaaa cgaccaatgc tatccgccag
     3121 aataactaac aacgcccccg ataccgcgct ccccagcagt aatgtcgtct gcccgccacg
     3181 tagaataaaa cgtgatatat ggggtgctat caatccgaca aaaccgatgt taccaacaca
     3241 cgaaacgcag acggctgtca ggattattgg tgcggcgaca cgtaacaacg ccagacgaga
     3301 actattcacc cctaacccgg ttgcaacctg atgaccaagc agagcgatat ccgccgtcct
     3361 ggcggtgaac agcaataaaa gagcggctgg caacatccaa caggctgaaa taccgactaa
     3421 catccagtta gctgcatgca ggcttcccga taaccacatc aatgcagttt gtacatctcg
     3481 aacatctgca gtggtcataa atacaccgat accggcggcg aaaaaccagg aaacaccaat
     3541 accaatcaga acaaatcgcg ggcgggaaat atcgcgcgca caaaaaatca cgattaaagc
     3601 caccaacaag ccaccagcaa gaccagccac tggacgccag aacattccca gcattggaaa
     3661 ctggaaaatc agccacagca ccgccacgct acatccttct ttgacgccga ttaaaccagg
     3721 atcggcaagg ccgttacgcg caatggattg catcgcggcc cccgccatac ctaacatggc
     3781 tccgcatagc aatgccatga tgactctcgg caagcgaata tcctgcacaa tataacgtgc
     3841 gtctgtgctt acgttttctg gagcaaacaa cgctcgtcct acttccgatg ccgggacaga
     3901 aaaagaacca tgcatcagac cgaatatcag tattccaacc gcaaacagaa acagcccacc
     3961 aagcaacacc agatttttag ggcgtaccag tgtcgaaaac ttaccaacac gtagtgggcg
     4021 tagcccgacg ttcattatcc gactcatttg aacatcctcg tcgccataat gacaaaaaca
     4081 ggcgcaccaa ctaacgccgt catgacaccg gtcgctaact catggggcgt gaaaagcgtc
     4141 cgagcaataa tatctgccag taataacaaa agcgcgccga cacaagctga aagagggagc
     4201 agaacgcgca gatccgctga taccagacgc cggacaatct gcggtactag caggccgaca
     4261 aaaccaatgg ggccagcaat cgatacggct gcgccacaga gcaacgcgat cgcaagcagg
     4321 gtgaatgttc gcgtgcggag taccgagacg cccagcccct gcgccatgcg atcgcccagt
     4381 gccaacatgt taagagaggg cgcaaggtaa atggctaaca caaatccccc cagagaaaac
     4441 caggcggacg tacccagcgt cacccaatcc tgccccgcta aatcacctgc tagccatgtc
     4501 cgcatcgcca gcagtgtttg ttcgtcaaaa atcaggattg cagccgttat tgaggacacg
     4561 aacgccgata gcgccacgcc gcataaagta actttcattg gcgttaaccc tgaacgccct
     4621 gcggaggaaa gcagcaatat caacaggaac aataacgcgc cccccgtaga tgccagcagt
     4681 gggcgtccaa ccggaaaggc cagtccaagc gcactggcgg caacgacagc aagagcggct
     4741 ccggcattta agcctaaaat atgtggctcc cccaatgggt tacgaatgac agcctgcaac
     4801 aaagcaccag caacaccaag agaagcaccg gtaagcaatg ctgccgctaa acgcggcaaa
     4861 cggagtctta caattacgtt gtgatcgaag ttttgcggat caaaatgaag aaatgcctgc
     4921 aacaccgttt gtggtgcaat aaatcgggca ccaatccctg gatataccac gcatcccacc
     4981 agcagtaagg cgaggaaaat tacagtaacc agccctgtgc gcatcatact ttttctacac
     5041 cacgaaacgg catgaagaac ggctttcccg tcagaggatt tattgatgcg tgaacatcaa
     5101 cgtcaaagac agcttttacc agttcaggcg tgcaatgctc cccctcattc aacacacgca
     5161 ccactttccc ctggcgtaaa aatatcaacg tatcgccata attgacggca aaattcagat
     5221 catgcaacac cacgacgact gtacgcccgt gatgacgcgt taaatcatgt aataactcaa
     5281 gaatttcaac ctgataacgt aaatcaagcc aggttgtggg ttcatccagc aggatataag
     5341 gcgtcttttg cgccaacacc atcgcaatcc agcaacgctg gcgctgccca ccagagagtt
     5401 tttcaacagg taaatgtgca aactcctgcg taccggttaa tttcagagcc tcttctaccg
     5461 cctgctcgtc ggcgtcgctc cattgacgaa tgaaattctg ccagggaaaa cgcccacgcg
     5521 acaccagttc ataaacggtt aagccctccg gcaataatgg cgactgcggc aagatcccaa
     5581 gtctacggga taacgctttc gttggttgct cgtgaatcgc tttgccatca agcaatatcg
     5641 aaccgcccat aggttgcaag atacgtgcaa tggtgctgag caacgtcgac tttccacagc
     5701 cgtttgcccc aaccagaacg gtcatttttt ctgtgggaac ggagaaagaa acaccatcaa
     5761 caatgatctt cttgtgatac ccggcagaaa cattgtcgag gatcagcccc tgcccttttg
     5821 cgttatgtgc cacgtgtacg ccaacttaat caaagacaaa caaaaataaa actcattctc
     5881 ctttgagagt ttgacgccac aaaatgtagt ag
//
LOCUS       DAZGHF010000214         5901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGHF010000214 DAZGHF010000000
VERSION     DAZGHF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.215"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..308
                     /locus_tag="ACHK3A_004697"
                     /pseudo
     CDS             1..308
                     /locus_tag="ACHK3A_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(507..1631)
                     /gene="yhhJ"
                     /locus_tag="ACHK3A_004698"
     CDS             complement(507..1631)
                     /gene="yhhJ"
                     /locus_tag="ACHK3A_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8742674.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1631..4366)
                     /gene="rbbA"
                     /locus_tag="ACHK3A_004699"
     CDS             complement(1631..4366)
                     /gene="rbbA"
                     /locus_tag="ACHK3A_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO8742675.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGSVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYHPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     ACLQEAAGQSNEAEAPPVIHDTTHAARQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMVPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4363..5430)
                     /gene="yhiI"
                     /locus_tag="ACHK3A_004700"
     CDS             complement(4363..5430)
                     /gene="yhiI"
                     /locus_tag="ACHK3A_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8742676.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(5493..5606)
                     /gene="yhiY"
                     /locus_tag="ACHK3A_004701"
     CDS             complement(5493..5606)
                     /gene="yhiY"
                     /locus_tag="ACHK3A_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO8742677.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
BASE COUNT         1377 a         1670 c         1526 g         1328 t
ORIGIN      
        1 atattctact gttgactatt tgtgccgtta tttctggtgc agaaggttgg gaagatatag
       61 aggattttgg ggaaactcat ctcgattttt tgaagcaata tggtgatttt gaaaatggta
      121 ttcctgttca cgataccatt gccagagttg tatcctgtat cagtcctgaa aaatttcacg
      181 agtgctttat taactggatg cgtgactgcc attcttcaga tgataaagac gtcattgtaa
      241 ttgatggaaa aacgctccgg cactcttatg acaagagtcg ccgcagggga gcgattcatg
      301 tcatttctcg cgtcagtcct tgcggggagc gggctttcgt aatcttgccc tgaataccgg
      361 actgagtata aaaaagccgg ttaactgaaa ctgtccaggt tttggtggtc agttcataaa
      421 cgccttatcc ggcctaaaaa aaactaaaat tcaataaatt gcaccaatgc gtagacccta
      481 taagcttacg catcgggcaa ctgtaattac gccattgtcc caattgtctt cctgaatctc
      541 agcagtgcga tggtgaaaaa cgcgccgcca attgccatca gtgtcaggaa ctgcggccag
      601 acgatttcga atccggcacc acggtagaga atggcctgcg cgaggctaac aaagtgtgtc
      661 gtcggcatgg tcagcataat gtcctgcacc atctgcggca tactttcgcg cggcgtggaa
      721 ccaccggaaa gcatttgcag cggcagcagc accagaatca ccagcagccc cagttgcggc
      781 attgaacgcg ctatcgtccc cataaaaata ccgattgacg tggtggcaaa cagactgagc
      841 gccacgccca gcataaacag cgggatcgag ccttctatcg gtacgcccag cacgcctttt
      901 accatcagca ccagcgataa tcccgacacc accagcacca ccagccccat cgaccagacc
      961 tttgccatca tgatctcaaa cggcgttatc ggcatcacca gtaagtgttc caccgtgccg
     1021 tgttcacgct cgcggatcag cgccgatccg gtcaatacaa tcgccagcat ggtaatgttg
     1081 ttgatgatcg ccatcacccc gccaaaccac gcgggatcaa ggttcgggtt aaagcgcatc
     1141 cgtgtttcca gcgataccaa cggttcgctg ttatcacggt agcgcgcgac aaagctgttc
     1201 acttcaccgt tgataatatt ctggatatac ccattgccgg taaatgcctg gctcatgcgc
     1261 gtggcatcga cgttcacctg aatatccggc tggcgtccgg cgaggacatc acgctgaaaa
     1321 ttaggcggaa tatttatcgc gaaggtataa cgtccggcgt ccagcccggc atccatctca
     1381 tcggcggtga tcatctccgg tggcaaaaac cacgggcgat agaagctgtt aacgatccgg
     1441 ttcgataact gcgattgatc catatcggca atggcgatcg gcgcgaggtt caacgatcct
     1501 ggcgtaacgg tcgctgacga atacaccgac accgtaaacg agaagacaat cagcgtcagc
     1561 atcgctttat caccgagcag actgcgcaac tctttgatac ccagattaaa aatattacgt
     1621 aaatggcgca tcatccctcc tgttttttca gcagcaggat gcttaagccc atcaccagcg
     1681 gtatggctat cagcaacggg ataaaaagtt gccacaaatc agtcagatcc agcgctttcg
     1741 agaacgttcc gcgggcgata gtcagaaaat gactggtcgg gtaaacctcg ccgatccaac
     1801 gtcctggacc ttccagcgaa gctaccggat cgatcatccc ggaaaactgt gtcgccggga
     1861 tcaacgtgat aattgccgta ccaaaaatgg cggcaatctg gcttttcata aaggtggaga
     1921 tcagcagccc cattccggtg gcgatgatga tatacagcag cgccgccagg gtgagagtca
     1981 ggaaactgcc tttatgcggt acgccaaata caaataccga cagggcgcag agcaggaaaa
     2041 agttcagcat ccccagcgcg atgtatggca gctgtttgcc aagcaaaaat tcgctacggg
     2101 tggtgggggt cacgtaaagg ttgataatcg accctagctc tttttcccgc accacgctaa
     2161 gggcgcttag cattgacggg accatcatca gcagaagcgg gatcaccgcc ggaacaatcg
     2221 ccggcaggct ttttacgtcc gggttatagc gatagcgcgt ctcaatattc atcagcccgc
     2281 tttggctggc gggtgtggac tgtcggctcg ccacatcctg tagccagctc tggtgcatgg
     2341 cctgcacgta gccttttacc gtttcggcgc ggctcggcat cgcaccgtcg atccagacac
     2401 cgagttccac gggcgtacca cgcgcgatat cgcgcccgaa attaggaggg atctcaattg
     2461 ccaccgtgat atcgcccgca cgcatccgac gatcaagctc gtcataactg gtgagcggcg
     2521 gctgttcgat aaagtaacgg gaaccggaga gattgagcgt ccacgcctgg ctactgacgg
     2581 tttgatcgcg gtcaagcacc gcaaagcgca ggttttccac atccatactg atgccgtaac
     2641 ccattatcag catcaggatc accgttccca tcagcgccag cgtcgaacgt accggatcgc
     2701 gtcgcagttc cagcgcttcg cggcggctgt agctaaacag acggcgcagg ctaaatccct
     2761 gacgcgccgc gtgggtggtg tcgtgtatca cgggcggcgc ttcggcttcg ttgctctgcc
     2821 ctgccgcttc ctgcaaacag gcgataaatg cctcttccag acttgcggct ccgcgtttct
     2881 caaccagttc ctgcggtgtc ccgctggcga gcacttttcc ggcgtgcatc agtgagatac
     2941 ggtcgcaacg ttccgcttcg ttcataaagt gggtggagat gaagatagtc actttgtcct
     3001 ggcgcgagag atcgaccatc aactgccaga acatatccct cgccaccgga tcgacgccag
     3061 aagtaggctc atcgaggatt aacatctccg ggcgatgaat caccgccacc gccagcgaaa
     3121 gccgctggcg aatgccgagc ggcaatgact ccggcagaac atcttcaacg tcgttgagct
     3181 taaaacgctc gctcatttca gccactcgcg cgggaatttc cgcttccggg atgtgaaaca
     3241 aacgggcatg taactcaagg ttttgccgca cggtgagttc gttatagagc gaaaacgcct
     3301 gcgacatata gcctacccga cggcgggtat cgatatcttt tggatcaacc ggttgcccaa
     3361 acagccacgc ctcaccttcg ctggcgggca gcagtccggt gagcattttc atggtggtgg
     3421 atttaccgca gccgttcgaa ccgaggaatc caaaaatctc cccgcgtgga atgcggaaat
     3481 tgacgtgatc aacggcaacg aaggaaccaa aacgcatggt cagatcgcgc gcttcgatgg
     3541 caatctctgc gttttcaggg tgatacggtg ggatcactac cgcctgatgc gcctggcgtt
     3601 gcgcttgcgg taacagattt ataaatgctt cttccagcgt ggcgctttgc gtttgctgcc
     3661 gcagctcttc ggcgctgccg gttgccagca cttctccggc attcatcgct accagccagt
     3721 cgaagcgttc ggcctcttcc atgtaggcgg tggcgaccag cacgctcata ttgctctgcc
     3781 gctggcgaat gctgtcgatc aaatcccaga actgggcgcg ggagagcggg tcaacccccg
     3841 ttgttggctc atcaagaatc aacagttccg ggtcgtggat taacgcacag cacagcccaa
     3901 gtttttgctt catcccgccg gagagtttcc ctgccggacg atcacgaaac ggtgctaacc
     3961 cggtgctggt cagcagctca ttgattcgca cttcccgctc cgctttgtcg tgaccgaaca
     4021 ggcgagcgaa aaaatcgacg ttttcataca ccgacaaggt gtggtagagg tttttgccca
     4081 gcccctgcgg catccaggcg atgcgcgggc aaacgtcgcg gcgatgcttc gggtcgcgca
     4141 tatcgccgcc cagcaccatc acgctgccct gctcaatgac gcgggcaccg gaaatcaacg
     4201 acaacaagct cgatttaccg acaccatccg gtccaatcag cccgaccatg caacgggccg
     4261 gaatatcgag tgtgatattg ttcagcgcaa cggtttttcc ataatgctgg ctcacgcccg
     4321 ccagttgcgc gacaggcggg acgggaacca gttccagatg cgtcattgcg gcaacctcac
     4381 caccaggtcg tcaggccacg gaagttcttc attcacccgc acccacgcta cgcccggcaa
     4441 tccggttttg acatattcca gatgctgctg gagtaattcc ggtgggatac gcgctttgac
     4501 acggaacatc aatttcagcc gctcatcgct ggtttcgacg gtttttggcg tgaactgggc
     4561 gacgctggcg acaaaactga tggtcgcggg aatacgcaga tctggtgcag catcaagaat
     4621 caaacgtgct tcaccgccca gtttcagcgt gcccgcctgt tcggttggca gaaagaaggt
     4681 catatagacg tcgctgagat cgaccatatt cagcacccga ccgcctgccg ccagcacttc
     4741 gcctggctcg gcaacccgat actgcacgcg tccgtcacgc ggggctttca gttcgctgtc
     4801 atcgatatct gcggcaatgc gccgttcagt ggcttgtgcc gcttcgacgc gagtttgcgc
     4861 ctgaatgata ttggtgcgtg ccgcctctat agcggcttta gaagcagaaa cctgagcttt
     4921 tgccgattcc agcgcagctc gggcgctctc agcggcggcg cggtcgtcat ccaactgttg
     4981 cgcagaaatt gccccccgtt gtgccagtga gcgggaacgc gtatgacgtt ttgctacgga
     5041 gtccagttct gcctggcgtt gattaaccag cgactgtgcg gcacgagttt cgctttggcg
     5101 ttgctccagc aaagcctgcg cggcggcaac ggcactttgt gcttctttga tttgcgcgat
     5161 ggcttccagt cgctgttcct gcaacacgcg agtatccatc ttcgccagca cttcaccttc
     5221 tcgaacaaac tggccttctt tcaccagaat ggtgtcgata cgcccggcaa ttttgctggc
     5281 gatatccact tccgtcgctt caatgcgccc attgctgaca gcaaagcctt ccggcacacc
     5341 cgccgggcgc aacagccacc acgccacgac ggcagccacc gccagtgccc cgacaaccca
     5401 ccacgccaga tggcgcttac tcttatccat aatcgatccg ccataatccc tgtaagcaat
     5461 cgacgcgtgt tacaactgcc accgaacgat gttcagcgga ttcttccatc cgggataaga
     5521 aggaaatggc aaatacgacc agcgcacaga gcattgagcg ccaatgggga aggcttagtg
     5581 aaaacgggta gtaatgttgt catcatcagc attcctggcc gtaaatgaaa cacgcatact
     5641 cataagtgaa atcaattaaa tactaacagt tatattcatg ttgtctattt gattgccatc
     5701 aacttgtgat acatcgcgat gttcttccgt tcgctggtgt tttttccaaa aatattctgt
     5761 acaggttcac agattctgtt gcacaggttt ccgtaacagg gaaacctgat gactatcaca
     5821 tagcgttatt atcgcctgat gtgcgaataa ataccgaata taaaataatt tattctgaaa
     5881 agatgataga tgataaaggc t
//
LOCUS       DAZGHF010000215         5889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGHF010000215 DAZGHF010000000
VERSION     DAZGHF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.216"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..347
                     /locus_tag="ACHK3A_004702"
     CDS             <1..347
                     /locus_tag="ACHK3A_004702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742678.1"
                     /translation="TICFLLVFAISGCVNPYNYREEQNVIISFKTNSPPQEIQECILS
                     EWQRTPLLATITSQKIGKYYSVLAVGDNADIYKLADGSTIIDFYSLRGVLDPTNGKSK
                     RIQGIKSCISTK"
     gene            complement(454..609)
                     /locus_tag="ACHK3A_004703"
     CDS             complement(454..609)
                     /locus_tag="ACHK3A_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8742679.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(907..1470)
                     /locus_tag="ACHK3A_004704"
     CDS             complement(907..1470)
                     /locus_tag="ACHK3A_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742680.1"
                     /translation="MTQASLPGGFTIEEAKELHEDLVRSHISKALSGEKMKKKDLDAD
                     LCWIHGVIVQASWFVKASLEQNALTGNSQVIPDDGREQFETLIRFHADDKNHETLLLR
                     ANEGMNYRDPNVDLAWIFWKSSREHILPGKSDNNPALGDQVSELTMLVKRLASSLKSV
                     NKSSKLPDKAMDYLKRNGLISAEDILR"
     gene            complement(1465..1608)
                     /locus_tag="ACHK3A_004705"
                     /pseudo
     CDS             complement(1465..1608)
                     /locus_tag="ACHK3A_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1608..2051)
                     /locus_tag="ACHK3A_004706"
     CDS             complement(1608..2051)
                     /locus_tag="ACHK3A_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742681.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VVYMWDSERKDIDAPGYYRTEHLVFAESSVKQWGGRVVPLYTAPPAPIVPAVLERLRA
                     IVADPRALPRRKEWVSGQQYSYVLLENVEAMVDEACRAAMLQGDK"
     gene            complement(2053..2397)
                     /locus_tag="ACHK3A_004707"
     CDS             complement(2053..2397)
                     /locus_tag="ACHK3A_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO8742682.1"
                     /translation="MSKIDYQALREAAQNYRSMLAWYQEKPDSPNAEQDCDAALSAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRTVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            complement(2394..2633)
                     /locus_tag="ACHK3A_004708"
     CDS             complement(2394..2633)
                     /locus_tag="ACHK3A_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742683.1"
                     /translation="MFKQNEKSIAQIAEYIPRACRGMQLQEAKARLEKKIALYINDGC
                     DAAVLNAAFAPALNSHTRESFFSCIAAQIHKGGNQ"
     gene            complement(2626..2829)
                     /locus_tag="ACHK3A_004709"
     CDS             complement(2626..2829)
                     /locus_tag="ACHK3A_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742684.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVDKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
     gene            complement(2935..3120)
                     /locus_tag="ACHK3A_004710"
     CDS             complement(2935..3120)
                     /locus_tag="ACHK3A_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742685.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGEDDED"
     gene            complement(3113..3295)
                     /locus_tag="ACHK3A_004711"
     CDS             complement(3113..3295)
                     /locus_tag="ACHK3A_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742686.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            complement(3391..3993)
                     /locus_tag="ACHK3A_004712"
     CDS             complement(3391..3993)
                     /locus_tag="ACHK3A_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO8742687.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLADVRAEVFND
                     LCAAFVRHAKVAGLDDADTVTFKEATEALLHCAEQLRAPE"
     gene            complement(3980..4285)
                     /locus_tag="ACHK3A_004713"
     CDS             complement(3980..4285)
                     /locus_tag="ACHK3A_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO8742688.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKQEGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            complement(4282..4563)
                     /locus_tag="ACHK3A_004714"
     CDS             complement(4282..4563)
                     /locus_tag="ACHK3A_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8742689.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            complement(4596..5312)
                     /locus_tag="ACHK3A_004715"
     CDS             complement(4596..5312)
                     /locus_tag="ACHK3A_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO8742690.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLIKAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPVADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEDIKTETVADIVQSLPSFTETRADDLVLPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDILRDITATRELLR"
     gene            complement(5346..5807)
                     /locus_tag="ACHK3A_004716"
     CDS             complement(5346..5807)
                     /locus_tag="ACHK3A_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO8742691.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISGSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(5800..>5889)
                     /locus_tag="ACHK3A_004717"
     CDS             complement(5800..>5889)
                     /locus_tag="ACHK3A_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein I"
                     /protein_id="HFO8742692.1"
                     /translation="YGGGVFEPLDSEERGRTFEALDCPDWRDD"
BASE COUNT         1323 a         1690 c         1238 g         1638 t
ORIGIN      
        1 aaacaatttg ttttctattg gtttttgcaa tttcaggatg cgtcaaccct tataactaca
       61 gagaagaaca aaatgttatt atctcgttta aaacaaattc tccaccacaa gaaatacaag
      121 aatgcattct cagtgagtgg cagcgtacac ctctcttagc cactatcacg tctcaaaaaa
      181 ttggcaagta ttacagcgta ttagcggttg gagataatgc cgacatttat aagttggcag
      241 atggttctac tatcattgat ttttattccc ttcgaggagt cttagacccc accaatggga
      301 aaagcaagag gattcaggga attaaatcat gtattagcac caaataatca tcagttcgaa
      361 cccagaaaca gccaaataga ttttatctca ggtgcgttga ggatgcctga cacatcagag
      421 gtggcgaggg atttctccct cgcctggtca ctcttacttc tcagattcgt agtctacgaa
      481 gacagcaacc tccgtctggc cggttcggat tcgtacctcg cagaggtctt tcctcgttac
      541 cagtgccgtc actatgacgg ttaaacagat gacgatcagg gcgattaaca tcgccttttg
      601 ctgcttcata gtctgcttct ccttgacctt tcggtccgta agaggctaac ctacgtttgt
      661 gaagcataga ttgggcctca gattaatgtt aagcgtcctg caagacgcgt aatgttaact
      721 ggggcttttc tctgtctgcc ttacggcggc atgcccgagg cagacagcct caagcacccg
      781 cagcacttct acttaactct tctttccccg caaaccgttt ttatccccag cggcaaatcg
      841 aatacaccac cagcaccagc gccaccgcca ttgcaattcc taccgccacg aatgcctcag
      901 gccaggtcat cgtaaaatat cctccgcgct tatcagtccg tttcgcttca ggtagtccat
      961 cgctttatcc ggtagtttgc ttgatttatt gacgcttttt aatgaactgg ctaatcgctt
     1021 gaccaacata gttaattcac tcacctgatc gcctaatgct ggattattgt cgctttttcc
     1081 cggcaatatg tgctctcgac tggatttcca gaatatccac gccaaatcca catttggatc
     1141 ccggtaattc atcccttcgt tagcacgaag caataatgtt tcatggtttt tatcatctgc
     1201 atggaacctg ataagtgttt caaactgttc gcgaccgtca tcagggatta cctgagagtt
     1261 acccgttagc gcattctgct ccagtgatgc ttttacaaac caggatgcct ggactataac
     1321 cccatggatc cagcacaaat cagcatcgag atccttcttc ttcatctttt cgccacttaa
     1381 agccttgctt atgtggctgc gcaccaggtc ttcatgtaac tccttcgcct cctcaatggt
     1441 gaaaccgcca ggcagactag cctgagttat cggagagttg ccagccttgc gcatggcaat
     1501 ctccatgatt tcaaccatat cccctggtgg aattttacaa agttgcccaa tacatttctg
     1561 ctgcctggca tattcgagaa tgtgctccag tttggttcga ttaatcatta tttatctccc
     1621 tgaagcatgg ctgcgcggca ggcttcatca accatagctt ctacgttttc gagaagtacg
     1681 taactgtact gctgcccgct aacccattct tttctgcgag gtaatgcgcg tgggtccgct
     1741 acaatagctc gcaaacgttc cagaacagct ggcactatcg gcgctggcgg ggcggtgtaa
     1801 agtggaacaa ctcgccctcc ccattgctta acactactct cagcgaacac taaatgctca
     1861 gtccgataat acccaggagc atcaatatct tttctttcgc tatcccacat gtaaaccacc
     1921 ggttctgctt ccagcgatgc cagtgcgata agcgccagct cacgaatttc accgccgtct
     1981 atatcgtcaa tgtcatcgcg gccagaaatg ttagccagcc attgcagtcg ctctttggta
     2041 atagtggtca tctcactctc cttctatgcg aataccagca gcagtcagtg ctgcattgac
     2101 ctcatcagcg tagtagtacg ttaatccact tgatgattta gccagcttga atggttctgg
     2161 taacttcact gtgcgggact cctgctcgtc gatacgctgt tttgcttcct ccagttcatc
     2221 cagcaaatca gcgataatat ccacttcacg atgacggatt tctcgcttaa acgcagacaa
     2281 agctgcatca caatcctgct cagcgtttgg actgtctggt ttctcctgat accacgccag
     2341 catcgaccga tagttttgtg ctgcctcacg caatgcctga tagtcaatct tgctcactgg
     2401 ttgcctcctt tgtggatctg cgctgcgatg cacgaaaaaa aagactcccg cgtatgactg
     2461 ttaagagctg gtgcgaacgc cgcgttaaga acggcagcat cacagccgtc attgatatag
     2521 agcgcaattt ttttctccag gcgcgctttg gcttcctgca actgcatacc ccggcacgca
     2581 cgcgggatat actcagcaat ttgagcgata gatttttcgt tctgtttaaa catgcttcac
     2641 ctcgacaggc ttgatggtgt cgatcagtag tcggcggcgc gtattttctg caaagtggcg
     2701 gcgtccggtt tctttgtggt aaaactcgtt tttgtcgacg acccacatcc gctccgtctg
     2761 gtgcagtttt tttacctgcg gaccgtcttt ggtgatcacg atgccggtat gggtttttac
     2821 gattgtcatg ccaccacctc ttcgaatttc aattccaatt gttcacccca gatttcacat
     2881 gactctgaac acgagccggt atcgaatcgc ctggcctgca ccatcgcctg atagttaatc
     2941 ttcatcgtcc tcaccccact catcacaata agcttcaacc ggagtttttc ctgcttcata
     3001 gtcatcacgc catgcttcag catcggcggc acttccaccg cgtaactctg catagtccat
     3061 taacagttca tgccattctt caaaactggc gttatattta gttgaaccaa aatcagccat
     3121 tttgttcttc ctcttcgtct tttatttcgt gatatgagta attgcagtag ttaaagaaaa
     3181 tttcttttgc ttcgtcatga atttcatcag gtgttgcgtc atcgtccact tcgaatacat
     3241 cctcaaaatc tccaccggct attcccgttt caataattat tttgaacttt cgcatttcac
     3301 taccgccctg ccgggcggtc tcctgatgtt ctgagggtgc aggaatccct ccggttaagg
     3361 attaaatttt atttacagtg ctaaatttaa ttattcaggt gcgcgaagtt gttctgcaca
     3421 atgcagcaat gcttctgtcg cttccttaaa cgttacggta tcggcatcat ccagtcctgc
     3481 aacttttgcg tgcctgacaa acgccgcgca aaggtcgtta aacacctccg ctcgtacatc
     3541 agccaggaaa gcatcggtgg ctggcatatt tcctgttgcc ttcatggcct ccaaaataac
     3601 cagaacgcca tctctcccaa cctcctcgca aataacctcg gtgttgtcgc caacaacatc
     3661 gcagaatgcc tgaactgctt tacgagcaag tgcattctcc gcagccagcg ccacaagatt
     3721 agcttccagc tcggcaatac gtttgctttg ggcttcccgt tcatccagca gtgcctgtac
     3781 tacttcaggg ttgaaagctg caataaattc tgcgttgttc tctgcatttt tctggtcatc
     3841 aaagccaggc catttgataa tgtctccgca acgttcatcc cccggcgtat gcaccgcata
     3901 tgtatcagtg cccggcgaaa tgaatgcgac ccattcgccc tgggttgcca gttttgctat
     3961 ctcacgtagc gtctgataat taatttttct cactggttgc ctcctggaaa ataactgcat
     4021 gccccagttt ctccgccagt gccagttctg ccttagcgcc tgccgaccgc tgccagcctt
     4081 tcagcatata aaccgcatcc acacaacgaa tcattgccat acaaatatcc atgtaatgtg
     4141 gctgagtcag cccgtccgga agtactgcgg gatttaatac ggtatgccct tcctgtttca
     4201 gtgcttcttc cgccttgtga aacgcctcac ggttgaaatt ttcatatccc gtcatcggac
     4261 cggcgatata aattctcacc ctcacgcctg aaccctcctg tcgaaataaa cgtagttatt
     4321 cactgcgccc aacttcatcc caaacttttc ggcaatttcc cgtcggggta cgccacgctg
     4381 atgcagttgc cgcgccagct caatatcacg ctgtgaacat ttggctgact ggtgataatc
     4441 accccgtaac atcatgcaga tacccagttc ccgcgctttc gtcctgacgg cagccccact
     4501 acggccaatc agactgccga tgctttcgac tgtcatcgtt cccgcacact gccggagtat
     4561 cataatttcc gcccagcgcc acttcttcca gccactcacc gcagcagctc tctggtggcg
     4621 gtaatatccc ggagaatatc cctgtgtttg ttcagttccc gcagcgcggc acagactcgc
     4681 tcccacttct gaacctgacc ttttgcccgg cgcagctcgc ggttagccac atgcagcgat
     4741 ggtaaaacca ggtcatccgc tcgcgtttca gtaaacgatg gcaacgattg cacaatatca
     4801 gccacagttt ctgttttaat atcttcctgt gtttccgctt cccggactgg taacgcaaca
     4861 cctgctggct gaggaaaggc tttaccatcc gtttccgcta cggatgcagc ttccggctct
     4921 gccggtaaat cagcgaccgg tatgcagtaa cgaaatttac cgccctgatt cacgcgaatc
     4981 agacgccctt tgctgattgc catggccagc gatgaattcg cccggcggga ggtaatcccg
     5041 aacatcagtg ccagctcatc cgccgtttgt gggccatgtt gttcaatcgc cttaatcagc
     5101 atttgcgctg tcactttcgg taccggtgac actggttcac tttcaccagc ctgaatcagc
     5161 caccacatcg aacccttgtt atccgcttca ccgcggcgct tcagtttcca cagttcgttg
     5221 accgcatctt cgcggctgat tccaaggcgc gatgccacta cctgtgaaga ggcttttttc
     5281 agtgctttca gtgcgtcaaa tacggtttcc attaaaattt cctccgacaa aatcgtttct
     5341 cagattcaaa taaaaccagc tgccttccgg cgttcgtatt cctgtttcag ccgttcaatt
     5401 ggcgttggcc cttgcgggtg tttcgcccct tccagttgtc gtcgcactgg cggaacactc
     5461 atcccgttac caacatgctt tgcccatttc gtcagttgcc gttccgcaag tcgttttaac
     5521 tcaccctgcg tcatctggcg ctcaatccct ctggtacgca tttcgaggca gatgtggtac
     5581 agcacaggct gtggccacgg gtatttatca ctcccgtcgt atcgccagga ttcattgcgc
     5641 cagcgccggt actcttccat cacggcatcc accgtaagac caaatggatt tgccccactc
     5701 cccgaaatca gcgcaacaaa ctcagccagg tccgggggcc acgtttcacc cgcccggcag
     5761 cggtccatgc actgacggca gaccagccgg atttgctgtt cagtcatcgc gccaatctgg
     5821 gcaatccaga gcttcgaagg tgcggccccg ttcttctgag tccagcggtt cgaatacacc
     5881 tcccccata
//
LOCUS       DAZGHF010000216         5824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGHF010000216 DAZGHF010000000
VERSION     DAZGHF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.217"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..563
                     /locus_tag="ACHK3A_004718"
     CDS             <1..563
                     /locus_tag="ACHK3A_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8742693.1"
                     /translation="FDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTE
                     SELHELIRPLLRDHFQPALLARFQTVIYRPLTQSAMRIIVEMKLAQVCERLHCHYGLS
                     TSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYLAARQHPTYLSLTW
                     NDEDGIVIAFDGINSDYPQESIYGQC"
     gene            550..1191
                     /locus_tag="ACHK3A_004719"
     CDS             550..1191
                     /locus_tag="ACHK3A_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742694.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTRHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            1188..1415
                     /locus_tag="ACHK3A_004720"
     CDS             1188..1415
                     /locus_tag="ACHK3A_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742695.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            1494..4013
                     /locus_tag="ACHK3A_004721"
     CDS             1494..4013
                     /locus_tag="ACHK3A_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO8742696.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            4010..5692
                     /gene="tle1"
                     /locus_tag="ACHK3A_004722"
     CDS             4010..5692
                     /gene="tle1"
                     /locus_tag="ACHK3A_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFO8742697.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSVV
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PEFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFEPIRQTNTELQLPSE
                     LIFLADKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            5712..>5824
                     /locus_tag="ACHK3A_004723"
     CDS             5712..>5824
                     /locus_tag="ACHK3A_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO8742698.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYK"
BASE COUNT         1569 a         1165 c         1554 g         1536 t
ORIGIN      
        1 tgtttgaccg gggctttatg cgcgacggtg aggggcgtga aattgacttc cgtaatacgg
       61 tcattctgat gacctccaac ctgggcagtg accttctgat gcagcaactg agcgaaaagc
      121 cggagacaac ggaatcggag ctgcatgagc ttatccgtcc actgctgcgc gaccacttcc
      181 agccagcact gcttgcccgt ttccagaccg tgatttaccg tccgctgacg cagtctgcca
      241 tgcgcatcat tgtggagatg aagcttgcac aggtctgtga acgcctgcac tgccattatg
      301 gactgagcac atcggttgat gaacgtgtgt acgatgccct gacatctgcc tgcctgctgc
      361 cggatacggg tgcccggaat gtggagagtc tgctgaacca gcaactgctg ccggtactga
      421 gccggcagtt gctcagctat cttgctgcca gacagcatcc gacgtacctg tctctgacgt
      481 ggaatgatga ggatggtatt gtgatagcgt ttgatggaat caatagcgat tatccgcagg
      541 aaagcatata tggacagtgc taaagaaatt gcccgggata tgctggaggc cgtaaatatt
      601 aatggggatg ggtttctggg ggccgtcggg aagggactca tatctttgcc agtcagtctc
      661 ggttatatgg gatatgattt tatagatacc aggcataggc gggaaaacca gaatgacaaa
      721 ttcagaatgg ccagactggt agaaaaaggc attttcagcc gtgatattat tgggaaaatt
      781 atcagtattt gtcttgaaga ctttgtatcc cggatcaata tggaaaaaac aggctccatt
      841 gtgaagaacg tttcaggaga tctggcaggt agaatggcat ttgctgaatt gaccggggtt
      901 aaactgggag aggttctggt atccagaggg ctggctgctt tttttgcagg gagttttgcc
      961 gggttgttat tatctgttgg cgcagagaca tcgcgtgcga tttacacatc ccgttttctg
     1021 aaagacagaa atccggtgtt gcatgataag ctgaaagctc agggggattt ggatcttttg
     1081 tactttcttg ttgaagatat tgtgcgcccg tttgaacgag cctgtattgt cgcagaccga
     1141 aattcggctg agtttgaccg gatatgcgaa tattttttcg gtggtttatg aagataaaaa
     1201 gaacattact tataatgctt tcccgattta tccgtggcgc aggtatgggg ttaggtgcgt
     1261 cgggtattgt atttacagtc tggttctttt ttctctcatc tgctgaattt aaatatctgt
     1321 ggggagggct ctctgttgcg ttattttttg tcggttatct gatgtatcga tttgcatatg
     1381 catatattta cgatgaatgg gatgattatc agtaattctc tctgaaaata aagcttaacg
     1441 gtcatcatta tataattgca gttcaatacc ggtacagaca tggagttaac aatatgagtc
     1501 tgacagatac actttcaaaa actatctctg cttccctgaa tcgttaccat ttaattattc
     1561 cctcctgccc gtcaccactg gatgttgagc atttcagcgg cagggaggag atgagtcaga
     1621 cctattatta catcatcaat tttaccagta ctgacaacga cctggatgcc gggctgctgc
     1681 tgcgtaaatc agccacattt acaatgggta cgggagggct taaggagcag gtgagccaga
     1741 aagtagtaca tggtgtcatt acagattttc gccgtatatc gggctccgcc gaccaggcaa
     1801 aataccaggt gatactggaa ccatttatta aactactgga caggcagcat cgttctcacc
     1861 gatttttcgt taacaaatcg gtgccggagg tggtgacaga ggttctccag gagcacggcc
     1921 tgaaaggctg ggagtttgag ttccgtctca aaaaaacgta tccgaagcgt gaacagatta
     1981 accagtatca ggaaagtgac ctgcaattta ttcagcgttt gctggctgaa gtggggattt
     2041 tttatttttt caccctgcaa ccggacacgc agacggaagt agtgcatttt ggtgacagcc
     2101 aggctgcgct gacgtttgat aagacgctgg cgataaacag tccgtccgga atgaatgata
     2161 acagagcgga ttcagtgtgg gggctgaatg tgacgcacaa tgtggcagag gccagtgtca
     2221 ccacaaaggc ttataaccat cgtgaagcac aatatctgtt gcagtccgcc ccggcagaca
     2281 tgacgcatgg tgatggtgac ggaattaact atggtgaggt gtaccactac catttccgtc
     2341 atctggaacg gggggataaa atcgacccgg tgccggagac ggccaatttt tatgcccgcc
     2401 ttggtcatga acgttatctg gcggagcagg tgcggataac cggcaacagt acagacgcca
     2461 cgctggcacc tgctcaggtt ctgactatca ccgacagtct tccgccgacg ttgcctgcgc
     2521 tgctgcgcaa tccggtgttg cttacctgtg tcggtttttc tgcaagtcgc aaggatgccc
     2581 tgcaggttgt actgaagggc gttccttaca gtgaagttat ctgctggcgc cctcctttac
     2641 tgccgcgtcc gaaagttacc ggtaccatga cggcccgtgt gaccagtgca aaagaagggg
     2701 atatctacgc ctggcaggat gcatcaggta tgtaccgggt gaaatttgat gctgaccgtg
     2761 atgataaaaa tccgggacag gaaagcatgc cggtacgtct ggccaaaccc tacagtggtg
     2821 atgcttatgg ttttcatttt ccgctgattc agggaacgga agtggccatt gcctttgagg
     2881 aaggtgatcc ggatcgcccc tatatcgcgc atgccctgca tgattcacgt catgttgatc
     2941 acgttaccga taagaacggt acgcgcaatg ttatccgtac gccggcaaat aacaagttgc
     3001 ggatggagga caaacgcggt gaggagcata tcaagatcag taccgagttt ggcggtaaga
     3061 cacaacttaa tcttggtcat aacgttaatg cctcccggaa actcagggga gaaggcgctg
     3121 agctgaggac tgatgactgg ataagtatcc ggggaggaaa aggaatattt atcagtgcag
     3181 atatgcagcc tcaggcacaa ggaaagatgc tggatatgaa tgaagctatc cgccaactgg
     3241 agcaggcatt atctctggcc cgaagtatgg cgaaggccgc aactgctgca aatgctactc
     3301 agggagatat aagttgtcag caacggttga attcatcgct aacagatctc actgcgccgg
     3361 gaatgctgtt gcatgctcct gatgggattg gcatggttag tgcacgggcg ttacgaattg
     3421 cttcgggtag tgaaagcgtg ggcataatgt cgggagataa cactgatatt agtgccgggc
     3481 agtcatttac agttgtagca gaaggtgctg ttagcctgtt gagcagaaac caggggatgc
     3541 aactgttagc agcaaaaggg cgggtaaata ttcaggctca aagtgacgat ttgtcaatga
     3601 gttcccaaca gaatcttgat attcaaagtt ctgagggcaa agtgactgtt tctgctaatc
     3661 aggaactgat actggcatgt ggtggagctt atatcaaact aagcggtgga aatattgagc
     3721 tgggatgccc gggacagatc ctgttaaaaa gcacgaatat gcagaagatg ggaccaacaa
     3781 gtcttgatat tgcgtctgtt gaaatgccgc gagggtttgg tggcggtttt attcttaccg
     3841 atgaagcggg agtgccgcaa ccttcgacgc cttatcgatt aacaacggct gaaggtgata
     3901 ttctacaagg gatcaccgat gaaaatggta aaacagcgcc agtcaacacc tccattccgt
     3961 cagtagtaaa agttgagttt gggaaggtaa aaattcatgg agaaacagaa tgacaaaata
     4021 tcagggatat gacgtaacag atgccaccca taaaaccagt attcataatg actggaaagt
     4081 tgttgttgca aagaaaaaac cggcgcgggg tgtgacttta accattggta tatttttcga
     4141 tggtaccgga aacaatcggg agaatacagc atctcgcctg atgaaattta atgagtgtag
     4201 tgcagccaga cagggggtaa atcaaaaaga tgcacaaagc tgtgaggact ttcttgaaga
     4261 aattaacaag aacagtatct cgaatggcag ttatcgagga tattattcca atattcactg
     4321 gctaaattca ctttacgtag cagatcagac tttgttggaa agtcaggttg aagcacaaat
     4381 tcagacttac atttccggga tagggactgc ggctggtgaa gcagattcag tcattggcat
     4441 gggattaggc accagtattc tggatatatt tgaaggtgtg gtgacgaaaa ccgacgaggc
     4501 aatggaacga atcacccagg cgttatcaga atttatgggg ttcaatttaa atcctgattt
     4561 ttgtatcgca aaaatccagt ttgatgtttt tggttttagc cggggagcag cggcggcaag
     4621 gcactttgcc aatcgggtta tggaacagga tcctgctata gccagagcta ttgctaaagg
     4681 gctcagaggc gatttttatg atggtaaacc ttctggtgaa gtccgttttc tggggctttt
     4741 tgatactgtc gcagctattg gtggaatcag taatttcttc gatattaatg gacgtagcaa
     4801 cccgggagta aagctggaat tgcgtccttc agtcgtgaag aaagttttcc agattactgc
     4861 aatgaatgaa taccgatata atttcagcct gaacagtatt aaaggtatgt ggcccgaact
     4921 ggctttacca ggggcgcatt cagatattgg aggtggttac aatccggtag gttcacctct
     4981 gcaggaaaat gaaagtctgt ttctgagttg cccggaattt gaaattgtca gtgacgatac
     5041 cagagaaacg gatacgcgag tataccgtaa tgcagagcaa gccagaaaaa tgttgatgac
     5101 tctgccagcc ttaaaacata ttttgcctca tggaaaacta accaccaaaa ttcgttccgt
     5161 aggtgtgaac aacagcaatc aacgtcgggc tggtgtgata caaaagcagg tgggagccgc
     5221 agtatttttt gagcgtatgg cagtacctaa tgactgggct aatgtttgcc tgcgtgtgat
     5281 gctggatgct gctcaggaag caggggtatt gtttgaacca attcgacaaa caaatactga
     5341 gttacaactt ccatctgaac taatatttct tgctgacaaa gccattgctc agggaaaagc
     5401 ggtacgtttg gggcaagagc ctcaggcttt tactgaggaa gagttgtata ttatcggaaa
     5461 atacacccat tgttctgcca actggaatat tgagtctgat ggtaatttat gggtggatcc
     5521 tacaacggga gagattttta tccatcgctt tggtcctaaa ggtaataagg cgttcgtttt
     5581 tccgaataaa ccaaatgatc gctggattcg ttctgtatgg tacatggatg atcagcagcg
     5641 attgaatgat aacgcagtaa aaaatactaa agttatgatg tctggtgttt aacaccacag
     5701 ggtggcaata tatgaccaga gataaaatca tttttatcgc accattactg gcattgttaa
     5761 ctgggtgtaa ttcggttagc tcacagtatg ctgattcgga taagactgaa tggggttata
     5821 aaaa
//
LOCUS       DAZGHF010000217         5674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGHF010000217 DAZGHF010000000
VERSION     DAZGHF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.218"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            36..151
                     /gene="rrf"
                     /locus_tag="ACHK3A_004724"
     rRNA            36..151
                     /gene="rrf"
                     /locus_tag="ACHK3A_004724"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            206..282
                     /locus_tag="ACHK3A_004725"
     tRNA            206..282
                     /locus_tag="ACHK3A_004725"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Asp,seq:gtc)
     gene            444..1247
                     /gene="dkgB"
                     /locus_tag="ACHK3A_004726"
     CDS             444..1247
                     /gene="dkgB"
                     /locus_tag="ACHK3A_004726"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO8742699.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(1244..2158)
                     /gene="yafC"
                     /locus_tag="ACHK3A_004727"
     CDS             complement(1244..2158)
                     /gene="yafC"
                     /locus_tag="ACHK3A_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO8742700.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            2399..3199
                     /gene="yafD"
                     /locus_tag="ACHK3A_004728"
     CDS             2399..3199
                     /gene="yafD"
                     /locus_tag="ACHK3A_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO8742701.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3276..4046
                     /locus_tag="ACHK3A_004729"
     CDS             3276..4046
                     /locus_tag="ACHK3A_004729"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8742702.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARHLKNIATRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(4093..5451)
                     /gene="mltD"
                     /locus_tag="ACHK3A_004730"
     CDS             complement(4093..5451)
                     /gene="mltD"
                     /locus_tag="ACHK3A_004730"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO8742703.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5523..>5674)
                     /locus_tag="ACHK3A_004731"
     CDS             complement(5523..>5674)
                     /locus_tag="ACHK3A_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HFO8742704.1"
                     /translation="LPVILKNERQINVFLRTEDIDLINVINEETLLQQPEERFAWLRS
                     KKDRF"
BASE COUNT         1406 a         1405 c         1380 g         1483 t
ORIGIN      
        1 aaccggcgac aagcgggtta ataaaacaga atttgcctgg cggccttagc gcggtggtcc
       61 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
      121 ctccccatgc gagagtaggg aactgccagg catcaaattt agcagtaagc cggtcataaa
      181 accggtggtt gtaaaagaat tcggtggagc ggtagttcag tcggttagaa tacctgcctg
      241 tcacgcaggg ggtcgcgggt tcgagtcccg tccgttccgc cacttattaa gaagcctcga
      301 gttaacgctc gaggtttttt tcgtctgtat ttctattatt gccagaatcg caaaaatcct
      361 ctgcgtttta cgctcttttt ccgcaacagt ctgaagccca taatcacctc agttaacgaa
      421 aatagcatta aaagaggcat attatggcta tccctgcatt tggtttaggt actttccgtc
      481 tgaaagacga cgttgttatt tcatctgtga aaacggcgct tgaacttggt tatcgcgcaa
      541 ttgataccgc acaaatctat gataacgaag ccgcagtagg tcaggcgatt gcagaaagtg
      601 gcgtgccacg tcatgaactc tacatcacca ctaaaatctg gattgaaaat ctcagcaaag
      661 acaaattgat cccgagtctg aaagagagcc tgcaaaaatt gcgtaccgat tatgttgatc
      721 tgacgctaat ccactggccg tcaccaaacg atgaagtctc tgttgaagag tttatgcagg
      781 cgctgctgga agccaaaaaa caagggctga cgcgtgagat cggtatttcc aacttcacga
      841 tcccattgat ggaaaaggcg attgctgctg ttggcgctga aaacatcgct actaaccaga
      901 ttgaactctc tccttatctg caaaaccgta aagtggtcgc ctgggctaaa cagcacggca
      961 tccatattac ttcctatatg acgctggcgt atggtaaggc cctgaaagat gaggttattg
     1021 ctcgtatcgc agctaaacac aatgcgactc cggcacaagt gattctggcg tgggctatgg
     1081 gggaaggtta ctccgtaatt ccttcttcta ctaaacgtaa aaacctggaa agtaatctta
     1141 aggcacaaaa tttacagctt gatgccgaag ataaaaaagc gatcgccgca ctggattgca
     1201 acgaccgcct ggttagcccg gaaggtctgg ctcctgaatg ggattaagcc tctctgacag
     1261 ctcctccggg agctgttttt acatgctcgc taaggaaatc gataaaagcc cggatgcgcg
     1321 tacttaccgc acggtcgctg taatatacgg cactgaatgg catttccact ggcaatactt
     1381 tatctgccat taactccacc aattctccgc gagcgatttc tctgtcgatc atgtagtcgg
     1441 acaaacacgc aatcccgttg ccactcaggc aaagctgttt cagtgtttcc ccactattgg
     1501 atgacaaacc gtacttcacc tcatgtaatt gtccatcact acaggctatc ggccaggtat
     1561 tgagggaagc gggttcagtg aatcccaggc aaacatgttg ctttaaatcg tcgatcgttt
     1621 ctggcttccc gtagcgggaa atataatcgg gggaggcgat aatttttcga taactgttaa
     1681 ataacggcct ggcacgtaag ctggaatccg ttaacgtacc agcgcgtatc gcgacatcca
     1741 cttttctttc gatcaaatta ataatcgttt cggaggagac tagcgataaa gtgacttccg
     1801 gatagcgttc acgaaaaggc ttaatcaacg gcatcagaaa gtgcagcacc actggagttg
     1861 cggcatcgat ccgtaacagt ccacgcggtg tattacgcgt ctccataatt tctgattctg
     1921 ccgctgccat ctcctgcaaa attgactgta cgcgacgaaa ataacgctcg ccttcttccg
     1981 tcaggctaag ttgccgcgtg gtccgattaa gcaggctaac gccaagtttc atctccagct
     2041 ttttcaccgc ccggcttacc gctgagtttg cttgccctaa ttgttccgct gcccggctaa
     2101 agctgccgct ttcgacgact gaaacaaaaa tggcgagttc ttccgacgtg gctttcattt
     2161 ttgctcctgt tgcaaaatag aagagatatt ttgaatttaa ttgtcattaa accatcagga
     2221 tgcgagatat gtcatccgat ttaatgttct caataatgag caaatttctg accggtgtaa
     2281 gcacttgctt acatagcaat atacaattgc tcgttgaaag agtgagctaa aatccctata
     2341 acagtagaac cctcccgagt gcggaagggt tgacgtaata gaggtttcaa agtcaaaagt
     2401 gcgaaaaaac acctatgcca tgcgctatgt tgctggacaa cctgcggaaa ggatcttacc
     2461 gccggggtct tttgcgagca tcggccaggc attaccacct ggagaaccgt taagtaccga
     2521 agagcgtatt cggatcctgg tgtggaacat atacaaacag caacgcgctg aatggttgtc
     2581 ggtattaaag aactacggca aagatgcaca tctggtgtta ttgcaggaag cgcagacaac
     2641 gccagagtta gtgcagtttg cgaccgctaa ctatcttgcc gccgatcagg tacccgcttt
     2701 cgtgctgcca caacatcctt ccggtgtaat gaccctttcg gcggcgcatc cagtgtattg
     2761 ctgcccgtta cgtgaacgag aacccatttt gcgtctggcg aagtcggcac tggtgacggt
     2821 ctatccattg cctgacaccc gcctgttgat ggtggttaat atacacgccg tcaatttcag
     2881 tttgggcgtg gatgtctata gtaagcagtt acttcctatt ggcgatcaga tagctcacca
     2941 cagcggcccg gtcattatgg cgggagattt caatgcctgg agccgtagaa ggatgaacgc
     3001 gttatatcgc tttgcgcggg aaatgtcgct gcgccaggtg cgttttaccg atgatcagcg
     3061 ccgtcgggcg tttggccgcc cgctcgattt tgttttctac cgtggtctga acgtcagcga
     3121 agcttctgta ctggttacgc gcgcttccga tcacaatccg ctactcgttg aattcagtcc
     3181 cggcaagcct gataaataag gtatgtcagg tctgccacag ggcagaccaa cgtttggcgc
     3241 tgcccttttt aatcaaacaa taaaaggaca gcgcaatgac aacacaatcc caccatgacc
     3301 acgtagaaaa gcagtttagc tcgcaggcca gtgagtattt aaccagtacc gtacatgcat
     3361 ccgggcgaga tttacagcgc ctggcggtgc gtctggctga ttatcctgat gcaagtgtac
     3421 ttgatatggg ctgcggagca gggcatgcca gctttgtcgc tgcgcagaac gtgagcgcgg
     3481 tggtggcgta tgacttatct gcccaaatgc tggatgtcgt ggcacaagct gccgaagccc
     3541 ggcacctgaa aaatatcgcc acccgccagg gatatgccga aagtctgcca tttgccgata
     3601 acgcatttga tattgttatc agccgttatt ctgcccatca ctggcatgat gttggtgcag
     3661 cactgcgaga agtgaatagg atattgaaac ctggcggtag gctgattgtg atggacgtaa
     3721 tgtctccggg tcacccagtg cgcgacgtct ggttacagac ggtagaagca ttacgcgata
     3781 cctctcacgt acgaaactac gccagcggtg agtggttgac gttaatcaat gaagccaatc
     3841 tgatagttga taatttaatt acagataagt taccgctgga attttcttca tgggtcgcga
     3901 gaatgcgtac gccagaagcg ttagtagacg ctattcgcat ttaccaacag agcgcatcga
     3961 cagaggtgaa aacgtatttt gccttgcaga atgatggctc tttcaccagt gatatcatca
     4021 tggtagaagc acataaagcg gcataaataa aaaaggcacc gggggaatcg gtgcctttta
     4081 ttatctggtt tgtcaggaat ctggcatgtt gttgtttttc acaaacaacg tcagcttatc
     4141 gcctggttgc agattcgcag tgtcgctgtt ccagcgcatt acatctttga tgttcacgcc
     4201 gtggcgttta gcaatgcttg aaagcgaatc gcctttgcgc acacgatacg taatgctatc
     4261 gctgttgttt gccagtcgct gtgcgctact gcctgcacca atcgtcaaac tttggcctgg
     4321 cttcagctta gatccgcgca gtttgttcca ctgctgcaaa tctttggtgc ttacgccgag
     4381 acgtgaagcg atacttgaaa gcgtgtcgcc agagcgtacg gtgtaaacac ggctgttaag
     4441 cggcgtattg tcggcaacca gcgtcgactg tacagcagca atttcgcctg aagccagaga
     4501 ttcacgcagt tgatctgcat gcttctttgg caccatcacg tactgcggac cacttgcgcc
     4561 cagcgtggag cctttcacgc ctgcgttgaa tgtcttcagc ttgctgacgg aaatccccgc
     4621 catatctgca accttcgcca tttcaaccgg gctgctcagg tgcacacgtg ccagagcacg
     4681 gctttcatcg gtcgttggca gacgtacgcc ataacgcttg ctgtttttga gaatatcact
     4741 caatgccagc atcttaggca cgtactgctt cgtttcctgc ggcaacggta acgaccagaa
     4801 gtccgtggat ttcccacgcg ctttgttcgt tttaattgcc ttcatgactc gaccttcgcc
     4861 gctgttataa gcagctacgg tcagaagcca gtcgccgtca aacattttgt tcagacgctg
     4921 catcatgttc agcgcggcag ttgttgaagc aacaacatcg cgacgcgcgt cataattgcg
     4981 ggtctgtttc aaaccataat tgcgccccgt gctcggaatg atctgccaga tgcctgcggc
     5041 attggcgcca gacgttgcgt gaggatcaaa agcgctctcc actatgggta gtagtaccag
     5101 ttccataggc atgttacgtt ttttaacttg cccggctatc cagtacatat acggctctgc
     5161 ccgtaaagtt acatcgtgga gatagctctt attgcgtaaa tatttctgtt tctgttcgcg
     5221 aatccggtca ttttccggaa ttcccatctt tagctcgtcg ccaatgaaag cccacaagtc
     5281 accatctggc gcgatagacg tcccatcgtc catccatcgt gcctgacttg taaactttgc
     5341 tgcttcccct tgaccagctg cagaaaggct ctgtgcgtgc tgttgaacgt tgccggtact
     5401 ctggcaaccc acgagcagga cagaggcgag taatatcgct tttgccttca tgtgtgtgtc
     5461 aatagttgct taaaagacga ccgatcataa cggcgaacgg agccgatgac aagaaagttt
     5521 tatcagaacc tatctttctt tgaccttaac catgcaaaac gttcttcagg ttgttgcaat
     5581 aatgtttctt cattaattac attaattaaa tcaatatctt ctgttcttaa aaaaacatta
     5641 atttgccgct catttttcag aattacgggt agtg
//
LOCUS       DAZGHF010000218         5654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGHF010000218 DAZGHF010000000
VERSION     DAZGHF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.219"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(124..2706)
                     /locus_tag="ACHK3A_004732"
     CDS             complement(124..2706)
                     /locus_tag="ACHK3A_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO8742705.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADKAVSTDSVTLNKDKTT
                     LDQDVVINGDNKITAVTIETSDSDKDLNVTFGGHDITAASTVNQGFVEGVKVSGDKNV
                     VINATGSTITAQGEGTYVRSAMVISSTGDVVVNGGNFVAKNEKGSATGISLEGAQGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSVLNGFNGDATDNITLAGSNIINGRIETIL
                     IAQENKGTHTVNLKIKDGSVIGAANNKQTIYASASAQGKGSATQNLNLSVADSTIYSD
                     VLALSESENSAATTTNVNMNVARSYWEGNAYAYAYAYAYAGVYADDDKASSNLNINLS
                     DGSVWKGNVAGSTTTGSTGTTENQSATVSLSDSTWKGNVGDGLDGGARVTLKNSSWTG
                     DVGNAKTAVFLTDGSVWNGAVSINAPVDRASVPMGSVQPNKSSVSLQNGSVWNVTGAS
                     TVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAGS
                     LDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSVI
                     QAMAAAPANVANLQADTLSARQDAVRLSENDEGGVWIQYFGGKQKHTTAGNASYDLDV
                     NGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGIF
                     IDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAKL
                     SALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFSD
                     GNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVGI
                     NVTW"
     gene            complement(2748..3509)
                     /gene="hyxA"
                     /locus_tag="ACHK3A_004733"
     CDS             complement(2748..3509)
                     /gene="hyxA"
                     /locus_tag="ACHK3A_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO8742706.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVANAGTQALSLHKYK"
     gene            complement(3663..4457)
                     /gene="hyxR"
                     /locus_tag="ACHK3A_004734"
     CDS             complement(3663..4457)
                     /gene="hyxR"
                     /locus_tag="ACHK3A_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFO8742707.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            complement(4787..5350)
                     /gene="ipbA"
                     /locus_tag="ACHK3A_004735"
                     /gene_synonym="fimX"
     CDS             complement(4787..5350)
                     /gene="ipbA"
                     /locus_tag="ACHK3A_004735"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFO8742708.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKCRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT         1497 a         1342 c         1205 g         1610 t
ORIGIN      
        1 atgttcgggt taaatatcag caaatatcca gcatcatgaa tactggatat ttatagcctg
       61 caggcagcat actgctgcct gcagggggta taacagtcga caggggaacc gactgcttta
      121 ttcttaccag gtcacattga taccaactac accctgtagc ggattctcaa tgtcgtcacc
      181 tttggtgtag tcaaggcttg catatgctcc catgtttttg gtgatatcag cctgtacacc
      241 tgcacccacg cggaatgctg caccgtcaat gctggcattg acagactcat cacccagacg
      301 gactttgttg ccatcagaga attcgttcag tgcggccaga ttcagataag gtttaacggt
      361 cgagttgccc acagcgaagt cataacccac gcgcgtaccg gcctcaccca gcacagagtt
      421 atagctgtca gaatgaacgt ccacgccgtt gagttgataa tccacacctt ccagcgtcag
      481 agcagacagt ttggcatatg gctgaataaa caggccatta ccgtctttcc atgtgtaacc
      541 gcctttaacc atcgcaccaa aaccattggt gttaaagtca gctttgatgg taccgccacc
      601 attcatcagg cgaacatctg ccgtgttgct gtaatgacca aactgtgcag cagtatcaat
      661 gaagataccg ttgttatact ggcggctcag gtaagcgtgg aagctgtaac cttcagtgtc
      721 acctttgctc tgcatggtgg tcatgtcacc tttcgcagaa gacatcgcca caccggccag
      781 ccagctacca tcttcagtca tgaagcgggt atcaccaccc agcattacac cgtttacatc
      841 caggtcatag gatgcatttc ctgcggtggt atgtttctgt ttaccgccaa agtactgaat
      901 ccatacgcca ccctcgtcat tttcgctcag acggacagca tcctgacggg cggagagcgt
      961 gtcggcctgc agattagcga cgttagccgg agcagctgcc attgcctgaa tcacggagct
     1021 ggcgttggat gctttatgcg tgttttttac gaaataaaag ttaccatcag ctccctgtgt
     1081 caggtcatat tgatacagac caccttccgt tgtgcctgtg gcttttagag tgctgtcttt
     1141 gccggtagaa ataaatgccg tttgttcatt gataagatcc aatgaacccg cactgtaaac
     1201 acgcaagtca ccgctcgctt taccgctgat atccagggtg ttttcagaag aggcatcaac
     1261 aatcagagtg ccgttttgag aagcaaacgt gccagtattg actgtagcct tcgtgatatt
     1321 aaccgtactg tcttttactg ccagagcatc aacagttgag gctcccgtaa cattccagac
     1381 agacccgttt tgcagagata cactgctttt atttggttga accgagccca ttggtactga
     1441 tgcgcggtcc actggcgcgt taatggaaac agcaccattc cacacagaac catccgtcag
     1501 aaaaacagca gttttggcgt tgccgacatc accagtccag ctggagtttt tcagcgtgac
     1561 tcgcgcacca ccatccagac cgtcacccac gttacctttc caggtggaat cagacagact
     1621 tacggttgct gactggtttt ctgtagtacc ggtgctgcca gtagtcgtac tacccgcaac
     1681 atttcctttc cagactgagc catcggaaag atttatattc agattactac tcgctttatc
     1741 gtcatcagca taaacaccag cataagcata agcataagca taagcataag cattaccttc
     1801 ccagtaagag cgggcaacgt tcatatttac atttgttgtg gtagcggctg aattctcgct
     1861 ttcagaaagg gccaggacat cagagtagat ggttgaatca gcaacagaca aatttaaatt
     1921 ttgcgttgct gaaccttttc cttgtgccga agctgaggca taaattgttt gtttattatt
     1981 agccgcccca attactgagc catccttaat cttcaggttg actgtatgag ttcccttatt
     2041 ctcctgagca ataagtattg tttcaatccg accattaata atatttgagc cagcaagggt
     2101 aatgttgtct gttgcatcac cgttaaaacc attcaatacg ctaccctttt gagcaaaaat
     2161 tgccgtggag gcgttgctgg aactcttatt accttgagca tttatggttg tgccattaag
     2221 cgtgacatta tttccctggg ccccttccag agatatccct gtcgcactac ctttttcatt
     2281 ttttgcaacg aaattaccgc cattaacaac aacatcgcca gttgaagaaa tgaccattgc
     2341 agaccggaca taggtgcctt caccttgagc tgtgatggtg gagcctgtag cattaatcac
     2401 aacatttttg tcaccgctaa cttttacacc ttcaacgaaa ccttggttta ccgttgatgc
     2461 ggcggtaata tcgtgaccgc caaaagtaac attaaggtct ttatctgaat ctgacgtttc
     2521 aattgttacg gctgtaatct tgttatcacc gttaataaca acatcttgat ccagagttgt
     2581 cttatcttta ttcagcgtaa ccgaatcagt tgataccgct ttatcagcag cgaatgctgg
     2641 tgcagaaaga gccataacaa tagctgttgc taatgcgctg cggtaaaatg ccttactgtt
     2701 tttcatgaag aaattctcca ttctggtaac taatttcaat ttcccactta tttatattta
     2761 tgcagagata aagcctgcgt ccctgcattt gccaccacgg aatacctgtt ggtatttccg
     2821 ttttcacgca atgtcatggc attcgttgtt tccaatgcat ttccatcacc gtgaaaatta
     2881 ttgctgctca gcagcagggc ttcccgcaat gacatgctgt ccggtattcc ccaacgcttt
     2941 tttagcgact gctcggcaag ccatatctgt cttatccccg ttttgagaaa ctgcgcactg
     3001 tattcccagc tgtgtccggc aagaagcaac gacaacacct tcttttgctt ttccgggaag
     3061 cgttctgaca tgacgattgt ctttgtgcgg ttattcacct ctttcataaa gacagagtaa
     3121 ctgtccataa ctggcaaaat ttctgcatgt ttcagccctg aaaacccacc agaggcattt
     3181 gcaatactga tgacacgctc tactgacatt cctggtttca gtaacctcag gtttttgaga
     3241 acgccagaaa tactcacatc tggtggcaga aaaaagggtg cgacgcacaa caccaccacg
     3301 aatgagtgat cccgataaaa tcggaaccac atttcagcgc attcggcagg tcgccctgcc
     3361 gatatcacga cactctgtgg cctgtgccgc aatttattgc gtaactcctc ttctgttctg
     3421 atgcaggtaa cattgccgac agaaccggtt gatagagctg cgaactgcgc tggtacaatt
     3481 cctgagggac agaaaagaac gaaacgcatc cttccctcac agatgtcagt acgccagcac
     3541 gctgtacagg ctgtacagcg tgcctcccaa tcctgcagcc cagagcagta gaaatatttt
     3601 ggctcctaca ctgtagcgag tccgtatgcg ccgcagtggg gggtagtaac aacgctgctg
     3661 tgtcataaca atcctggtct ttttttagac atattttttc tttgtccgga aagtactttt
     3721 ccggatatgg cgtcagaatt tattgattca ccaacggcaa ctttctctgg ataaagaatt
     3781 ccggatacca tgttctgcac atactgagtg agtcgatgag cggaatgttg tccgatatat
     3841 tttgcatgta gccgtatatt ccgtttcact ctgctgtcac cgcatcccgt atagagcagc
     3901 ctgaaatcgc tcagtgttcc cagtccaagt ttcattaaag cccgtgtggc aatcgcatgc
     3961 agggtctttg aactgcgttg ttcataacgg gcaatctcac tgaaactcat accctgcatc
     4021 agaatcttaa gcacgctcaa ctcccgtgca gtcaggagcc agaaatcgct catatcgccg
     4081 ggcaaaggac gtagtaacca gcggataatt tgattctgat aacgactcgc tgtcagcatg
     4141 gtgccgatgc gtgggaagcc gttatacaga cttgcagcag gagcattatc gaaccagtcg
     4201 gagtcaccgg tcaggcagac ctgccattgc ccggcatcac tccgcaagcg gacaagacaa
     4261 gccagaagaa ccaactctcc cgaaggaagc gatgggatat cagctaacag gtagccacag
     4321 gaggtcttta aaacgaattc aacctctgta gtgacgttgc tgtacacccg cacgatatac
     4381 ctgtccgttg ggatgagaga ctccagtgcc agcctggtga actgacaggc actgataata
     4441 accagaggct tcttcattca gactcgtcca cgccattgct acaaaacctg acattttgat
     4501 tcaaaatgtc aggtttggtt taattgcctg aatatttaat tttattggaa tataaatcta
     4561 atttgtttga tttttattgt ttttaatttt gtgttattta acaacgcgag tgaaaactga
     4621 tcactggtat gacctttgtg gttaatcatt tcaaatcatg gggttatttt actttagcga
     4681 gaaatatcaa catgttatgt gttttattta attgaatgag ctgttttttt atggaagaat
     4741 tgaggcaaac gtaggaatct gacattttga atcagaaggt actgttttaa cgaggctttt
     4801 ttttccacac ccctttaaat cgagcagcat tactggctgt gtatctgaca gtatgctgaa
     4861 tatttcgatg acccagataa tcctgaagta gtcgggtatc gacaccattg tcagccagag
     4921 caaatccaca ggcatgacgt aacatatgag gatgtgcttt tacagctaat cctgcacgtc
     4981 ttcctgcctc acgaatgatc gaaaaaaaat gttgtctgct gatagggcgg catttccggg
     5041 agagaaaaag ccagtctcct tcaactccat tttcaattaa cttcctctgc ttaagccaca
     5101 acttgatcaa attatattca tcaggaagaa gaggatgtgt tgtcgagaag ccatttttaa
     5161 tacgccggat attaagttgt tttcctgatg catcaatatc ggataaacgt aaatcaagaa
     5221 gttcactggc cctaaaaccg tgaatgaaag ccatcataat tagacagcga tttctttcag
     5281 gaaaggacat gctctgggct gcatccatca gcctgtagac ttcatcctgt gtgagatatt
     5341 ttctcgtcat acgccaccgt gtacagttgt aaaaaaaaga ggcacaccca gataagtgtg
     5401 cctgcaatca gaacgttatc gatgtaattt cattatctca ttaataagat tttttgaata
     5461 gtagttctac gcaatagatt aaatatgaaa tatttattgt gcggtttttc tacaactcat
     5521 ttttaatgcg cgattttttc tacaatgatg tgaaacaaaa aatattaaaa ccctgagtca
     5581 aggagcgggg gtgaatgtgt attacgtcta atgttttagt gatgttcaat agtcttgatt
     5641 tatttaatgt tatt
//
LOCUS       DAZGHF010000219         5625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGHF010000219 DAZGHF010000000
VERSION     DAZGHF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.220"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(62..976)
                     /locus_tag="ACHK3A_004736"
     CDS             complement(62..976)
                     /locus_tag="ACHK3A_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8742709.1"
                     /translation="MDKTFSIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGVAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            complement(1036..1539)
                     /locus_tag="ACHK3A_004737"
     CDS             complement(1036..1539)
                     /locus_tag="ACHK3A_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8742710.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(1552..2082)
                     /locus_tag="ACHK3A_004738"
     CDS             complement(1552..2082)
                     /locus_tag="ACHK3A_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8742711.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            complement(2096..4747)
                     /locus_tag="ACHK3A_004739"
     CDS             complement(2096..4747)
                     /locus_tag="ACHK3A_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8742712.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGHEAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVSDNSACTPLRDRLADASSEFNVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKDADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEEYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTLFIDYFNLFYSKRGQEQISISQQLGHYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPAVSPETLLNQQTAICR"
     gene            complement(4789..5499)
                     /locus_tag="ACHK3A_004740"
     CDS             complement(4789..5499)
                     /locus_tag="ACHK3A_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8742713.1"
                     /translation="MQTTRPPYSVSFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAASGDISF
                     QTINDYGALTPRIKATMQ"
BASE COUNT         1532 a         1252 c         1228 g         1613 t
ORIGIN      
        1 caaaataatt atccgcgcaa ttttcgcgaa tggagcgcat agactaaggt caaaatcaca
       61 attattcata gataaaagtg acaccaatga ctgactgaac tgttccggct gaaactttgt
      121 ttcctgtttg tccataggtt gcgcttaaac ccagaggtac tttgctttta tttactgtcc
      181 ccagggagac gttttctccc gtagccagta tttttccatt acgcatcaga gagacaccca
      241 ctcccgatgc ttttgttgca tctggtgctg tgttagcaaa cacttgtctg gcactgtcag
      301 tggtagcacc cgaaagataa aatgagagct tttgttcact tgagcaatat acgccaaggg
      361 gtatttctgc gcttccagga aagtccggga gattcacgtt gacattacga ctatcaacgg
      421 tgcagccgcc tgttggcata acaacactgt tatttgaaat aatattccac gtaaaattac
      481 gcggatttcc gcttcctaac gtggcaattt tatacatatg aatacgggca ataacttccc
      541 ctgcttttat gacaactcca ccggccactc cgactggggt aatatagagt tttaacggta
      601 atggcatcgg tgtcttatca ccaatatcca atacgttggt atttgtcgtc aacgggaagg
      661 ggtaggtcac attattccag tacagtgatc ctttatatga ctgtagggag cctgcgaaag
      721 ctgatccttg taccaggttt atatgatcag tgtcgtacca gccgccgtaa tcattccagc
      781 aacttatatg ctgagacaag tctacaacca gattctggcc cggttgtatt acaggatcga
      841 gattaacata aacagaggtt gtacctgctc caatacttga cccgccatcg acattacaac
      901 tgaaggcaaa aactttgcct gccattaata gaagataaat accgaacagg acctttatac
      961 tgaatgtttt atccatgata tatcctaagg ttaaaaaatt gatttaaaaa ggaggcaaaa
     1021 aaaatcaaat tgatgttatt gccaggtata gatcacattg attagcgcct cgatcgttcc
     1081 ctggcttgcg tttccattca ccgtgatagc tcttgcctta agtggaaact gtgcattacg
     1141 agtgatctca tcaacaataa ccgttttgct atcgccattt tttaacgtcg catcctggtc
     1201 atcccttagc tctatctgaa tattttcggc agtaccttca tttttgtaat aacccgtatt
     1261 gtcagttgaa cctgtcacga ttgccgtcac tgcacttgtt tcaaccggac aatcggttaa
     1321 tgacaaagta atattgtgcc agcctgatgc agaaccaggt tgttgcagat tgcgcgtata
     1381 aagatccccg agattaacgt tagcttcttt ggtttgaata gtgcagggtt tagcgactac
     1441 ccgaccatta acagtgatag tgacatcggc ggcctggaga gttgctgtga ataacgcgca
     1501 aaacgtcgct aacaggaacc tcttgtgaag tcttttcata tctggctctg attattgaaa
     1561 ttcaagggta aaggttgccg aagccctcac cagtcccgga ttgacggact tctgagttga
     1621 cttcagacga gcggagtaag gcaaaatatt gttctgttct ggtaccagtg ggatccactg
     1681 catcccggca tgaaggtcat tcaatttcac cggacgcata tttccgtcca gtatttctat
     1741 acccaaaccg gaggccgtgt tgcttccttc atccagtttc aacagagtat tattttctgc
     1801 atcctcaata ccgttaaatg caacccgaac ccccgttgtc cctttactgc attcacttaa
     1861 cgtaatctga aaagggacgg ctggacttgt gctaccggtc gttggaaatt gtctggcact
     1921 gtttttttgg agatctacgg taaaattaag cgaatccgat gagactgtgc agccataatc
     1981 gagaacgcgc ccgctaattt taataacgct atctgcagat aaagcagaat aggtggttaa
     2041 ccccagacat aaaccgagga aaataacgtt attgtatttc ataatctatt gttccttagc
     2101 gacagattgc tgtctgctgg ttcagcaagg tttcaggaga gactgcagga agcttgtact
     2161 cgacaataca gtttgagttt ttatctttgc cccatgaaac ctgtaatttc cctgactgtg
     2221 gaagtccagt cagataaacc tgaccgtttt ccgcgacaat gctgccattt ttattctccc
     2281 catgtgtgac aattgcaccg aatggaacgc tcttattacc gtacttcaac gtcattaata
     2341 ctttcccgcc gatttgtgca ttaaatgttg ctctggcaat agcaccgtga gttgggatga
     2401 cagtgacaac ggtttcatcc agttcaacat tatctgcaag ggaattcgcg ttaagagcga
     2461 cacgattttc tctatattct gtcgcaaatg gtaatatggc atagccacgc cagtcggtat
     2521 gaattccggt ctggttctct attttgacat tatcagcgcc aggagcctta accagaacca
     2581 ttgtgtcgcc cagcggctgt ccaaaggtga tgccatcagc atgagcaata atgccaccac
     2641 tcattccgta ataaatctgg ctgctgtcac cactccgact gtaaccgaca ttcgtattac
     2701 cgtaagctcc acgataatta agagaactgt aaccactggt gccagacgat gtattacccc
     2761 cttgggtgtt accgacctga acgctataat tcaggttatt atccggcagc agagtgccat
     2821 aaacccctga taggttggtc atgccgcctt tcaaatcgtt tgacatactg taactggcgt
     2881 ttgaattacg aaatgccgac tgactgtctg tacgcatcca atgactgaag ggaacattaa
     2941 gcgtaaaagc aagtaaatga tcccgatcgt tttgccatat attattggaa tagctgtaat
     3001 tcagcgaagt cgtaatatca ccaaacggca catttaatcc aaatgatatt tgctggtcgc
     3061 tgcgtgacgt gttccagtaa ctttggcgac tggcactgaa aaatgtcgta ccgtaatgtc
     3121 caagctgctg agagatgctt atttgttcct gaccacgctt actgtaaaac agattaaaat
     3181 aatcaataaa tagtgtctgc tcattgctgt ctccggtagg aggcttgacg gtgtaaccac
     3241 tcattcgact gtaggcactg tcggataagt tataaaaacc ttgcgtagaa tagcgatatc
     3301 ctgcgacctg gatattggtt cccgtctggt agaaggattt gctgtagacg gatttaaccg
     3361 attgtccgct gtgacgggtg tcatctgcaa gcgtcgtatt ggcctgcgtg atatcgaaag
     3421 aaaaggcacc aaaaagtcct aaatctttac caataccaag gttgaaggcc tgatattctt
     3481 ctgcaatttg cattccgcca taaggtgtcc agtttccttc cagtccatgc atcaagctac
     3541 cttgtatgaa tttgggggag ctttgcaggt tatttccact acgatattcc cccatggcaa
     3601 gcgcataacg cgtatatcca gcacgttgga gaaccggaac agacgaataa ggaacatata
     3661 atgtctgaat actgccgtct gcatctttta tggttacttg cagatcacca ccattggccg
     3721 cagagttgat atcatcaata gtaaaagggc cgggtgggac agtagtctga taaacatcgt
     3781 atccattctg ttttacactc acttgtgcgg tgccgcgggc aataccatga atcaccggag
     3841 cgaaaccatg ttggctatcg ggcaacatcg cttctgttga atttattttg aggccacgaa
     3901 agtttacact atcaaaaata tcgccatcgg tataactatc acctaccgtt aagcgtgagc
     3961 gtaagggaat aatgtcacgt tcagccgacg tattgatatg ctgccattta ttgctgtcag
     4021 atgaattgct actcccactg ttataactcc acgttgagtt atcgcgcagt cgccaactac
     4081 caatgttgat gccactctgt aaattcaaat atgcatagtt ggattttcct gcattatggt
     4141 tactacgatt attaatagaa ttaccattaa aactatagtt tagtagccca gcatttattc
     4201 cttcttccca cagatccgga gaaacatagc cgcgagccat tctgccaaca taaatttgcg
     4261 gaacggagag tgatagatgt tgctgaccaa cattaaactc agatgatgcg tcagccaaac
     4321 gatcccgaag tggtgtgcat gcgctgttgt ccgacacatg tttttctgcc gaatgttcct
     4381 tattgtctaa taatgcagat ttttttatgc caagactgac aagaagatct gtactcaggc
     4441 atgggatgag atctgcgtta ttatcatctg caataaaagt aatatcccga ctagtcataa
     4501 attcgtcgtt caaataaatg tcgacccgat atgtaccagg tggtgcctcg tgtcccttag
     4561 taaaggcgga taaatcgaca gattcagcaa gatcattgct taaaaaccgt ggattaaaat
     4621 aatactctgc gtcaacggga aaaaccgcag ttgctaagcc aagggcaatg gtaagccgtg
     4681 caaagcgagg cagtagtaac acctgatgca tacgatatgc cttaaatgcg gcacgaaagg
     4741 ctgtcattat taattccgat tgagtttatt attaattaag tcactgcttt actgcatggt
     4801 tgccttaata cgcggagtta atgcaccgta atcattgatc gtctgaaaac tgatatctcc
     4861 ggatgcggca tgcgggatat ccaccgatac ttcacctttt ggcggcacca tggtgtttgg
     4921 gagattgcta tttcctgctt tcatattcgt tacggtaatg aagtagggcg ttggattaat
     4981 aagagtaagt ttgctaccgc tgcgacgaaa ccgaagcatt gcaggcgctt cttcaggtgc
     5041 cattgccagg ttcgtggggc gataaaacat ttttatgcgg ctaataattg ccagctgtaa
     5101 tgtattctca ttcttctggt ctttttccat agcaggaata gctttaacat tcacccagaa
     5161 caggctttca cggtcgcctg gtaactgatg attagtggcg ttgattatcc gcagcgtgtt
     5221 ctctttttta ccttgcatgg aaaaaagcgg tggcgtaatg acaaactggg tgtctttttg
     5281 gtcacccgca ttttctatcc atgactgaat gagatagaca ctggcaggat cattattcgt
     5341 gacaggcaat aaaacctgtt tctggtttgc cggataaatt acccgcgtcg cacccagtgc
     5401 gacagctgcg ttggcaacgg tgctatggca agcaaagagc aataaaagta acactgtcgc
     5461 cataaaggaa acagagtagg ggggacgcgt agtttgcatc tgatgcattc tccatgtgtt
     5521 aagaggataa gcggggaaac cgttacatca gacaatcggt aaatgttgct aatacattgg
     5581 cgagtcgccg ggtttccaga cttaatgcgt ttgaaataaa tgttg
//
LOCUS       DAZGHF010000220         5549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGHF010000220 DAZGHF010000000
VERSION     DAZGHF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.221"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            35..111
                     /locus_tag="ACHK3A_004741"
     tRNA            35..111
                     /locus_tag="ACHK3A_004741"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69..71,aa:Arg,seq:acg)
     gene            392..958
                     /gene="yqaB"
                     /locus_tag="ACHK3A_004742"
     CDS             392..958
                     /gene="yqaB"
                     /locus_tag="ACHK3A_004742"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO8742714.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            955..1383
                     /gene="yqaA"
                     /locus_tag="ACHK3A_004743"
     CDS             955..1383
                     /gene="yqaA"
                     /locus_tag="ACHK3A_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO8742715.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1456..3012
                     /gene="gshA"
                     /locus_tag="ACHK3A_004744"
     CDS             1456..3012
                     /gene="gshA"
                     /locus_tag="ACHK3A_004744"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO8742716.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFIAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            3162..3677
                     /gene="luxS"
                     /locus_tag="ACHK3A_004745"
     CDS             3162..3677
                     /gene="luxS"
                     /locus_tag="ACHK3A_004745"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO8742717.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3728..4840)
                     /locus_tag="ACHK3A_004746"
     CDS             complement(3728..4840)
                     /locus_tag="ACHK3A_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8742718.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALVTQPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWKQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(4837..5544)
                     /locus_tag="ACHK3A_004747"
     CDS             complement(4837..5544)
                     /locus_tag="ACHK3A_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HFO8742719.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINADYWQDLQAITQLVHTEK
                     LDGENNCLNRYGVFARSHAAPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGAFDPWDTQTGQPCTLEGIVSRNRDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFDYGEQS"
BASE COUNT         1340 a         1371 c         1482 g         1356 t
ORIGIN      
        1 tatcaatatc agcagtaaaa taaattttcc caatgcatcc gtagctcagc tggatagagt
       61 actcggctac gaaccgagcg gtcggaggtt cgaatcctcc cggatgcacc atattctccg
      121 taaccttcag caatgaaggt atcgaagagg tagcggtatt aaccgcaggc accagggagg
      181 ataacgttgc gccagcagcg gcccgaaggg cgaacgccag tgagtcatcc tcccggatgc
      241 accatcttct ccaaaaaatt tccttcaaaa atatctcatt tctaaataac tactttatcc
      301 atttcctgaa tattcttctc cttctccatc agcgacaaaa tcaagcaatt acgctaaagt
      361 agcgtcatat ttttcgactt gcgagaagac catgtacgag cgttatgcag gtttaatttt
      421 tgatatggat ggcacaatcc tggatacgga gcctacgcac cgtaaagcgt ggcgcgaagt
      481 attagggcac tacggtcttc agtacgatgt tcaggcgatg attgcgctta atggctcgcc
      541 cacctggcgt attgctcagg cgattattga gctgaatcag gccgatctcg acccgcatgc
      601 gttagcgcgt gaaaaaacag aagcagtaag aagtatgctg ctggatagtg tcgagccgct
      661 tcctcttgtt gaagtggtga aaagctggca tggtcgtcgc ccaatggctg taggaacggg
      721 gagtgaaagc gccatcgctg aggcattgct ggcacacctg ggattacgcc gttattttga
      781 cgccgtcgtc gctgccgatc acgtcaaaca ccataaaccc gcgccagaca catttttgtt
      841 gtgcgcgcag cgtatgggcg tgcaaccgac gcagtgtgtg gtcttcgaag atgccgattt
      901 cggtattcag gcggcccgtg cagcaggcat ggacgccgtg gatgttcgct tgctgtgagt
      961 gaagcgttat cgcttttctc gttgtttgcc agtagttttc tcagcgctac actattaccc
     1021 ggcaactccg aagtcgtgct ggtggcaatg ttgctttccg ggatcagtca tccctgggtt
     1081 ttagttttaa cagcaacaat gggtaatagc cttggagggt taactaacgt tatccttggg
     1141 cgtttctttc cattgcgtaa aacatcgcgc tggcaagaga aagctaccgg ctggctgaaa
     1201 cgctatggtg cagtcacgct attattaagc tggatgcccg tggttggcga tttactgtgt
     1261 ctgttagcgg gatggatgcg catctcgtgg ggaccggtaa tctttttttt gtgccttggt
     1321 aaagcgttac gctatgttgc ggttgcagca gctaccgttc agggcatgat gtggtggcac
     1381 taattgtagg cctgcacata tggtcaccat tacagttatg ctaattaaaa cgattttgac
     1441 aggcgggagg tcaatttgat cccggacgta tcacaggcgc tggcctggct ggaaaaacat
     1501 cctcaggcgt taaaggggat acagcgtggg ctggagcgcg aaactttgcg tgttaatgct
     1561 gatggcacac tggcaacaac aggtcatcct gaagcattag gttctgcact gacccataaa
     1621 tggattacta ccgattttgc ggaagcattg ctggaattta ttacaccagt ggatggtgat
     1681 attgaacata tgctgacctt tatgcgcgat ctgcatcgct atacggcgcg caatatgggc
     1741 gatgagcgga tgtggccgtt aagtatgcca tgctacatcg cagaaggtca ggacatcgaa
     1801 ctggcacagt acggcacttc taacaccggt cgctttaaaa cgctgtaccg tgaagggctg
     1861 aaaaatcgct acggcgcgct gatgcaaacc atttccggcg tgcactataa tttctctttg
     1921 ccaatggcat tctggcaagc gaagtgcggt gatatctcgg gcgctgatgc caaagagaaa
     1981 atttctgcgg gctatttccg cgttatccgc aattactatc gtttcggttg ggtcattcct
     2041 tatctgtttg gtgcgtctcc agcgatttgt tcttctttct tgcaaggaaa accaacgtcg
     2101 ctgccgtttg agaaaaccga gtgcggtatg tattacctgc cgtatgcgac ctctcttcgt
     2161 ttgagcgatc tcggctatac caataaatcg caaagcaatc ttggtatcac cttcaatgat
     2221 ctctacgagt acgtggcggg ccttaaacag gcaatcaaaa cgccatcgga agagtacgcg
     2281 aagattggta ttgagaaaga cggtaagcgg ctgcaaatca acagcaatgt gctgcagatt
     2341 gaaaacgaac tgtacgcgcc gattcgtcca aaacgcgtta cccgcagtgg tgagtcgcct
     2401 tctgatgcgc tgttacgtgg cggcattgaa tatattgaag tccgctcgct ggatatcaac
     2461 ccgttctcgc cgattggtgt agatgaacaa caggtgcgat tcctcgacct gtttatggtc
     2521 tggtgtgcgc tggctgacgc accggaaatg agcagtagcg aacttgcctg tacacgcgtt
     2581 aactggaacc gggtgatcct cgaaggtcgc aaaccgggcc tgacgctggg tatcggctgc
     2641 gaaactgcgc agttcccgtt accgcaggtg ggtaaagatc tgttccgcga tctgaaacgc
     2701 gtcgcgcaaa cgctggatag cattaacggc ggcgaagcgt atcagaaagt gtgtgatgaa
     2761 ctggtcgcct gcttcgataa tcccgatctg actttctctg cccgtatctt aaggtctatg
     2821 attgatactg gtattggcgg aacaggcaaa gcgtttgcag aagcctaccg taatctgctg
     2881 cgtgaagagc cgttggaaat tctgcgcgaa gaggatttta tagccgagcg cgaggcgtct
     2941 gaacgccgtc agcaggaaat ggaagccgct gataccgaac cgtttgcggt gtggctggaa
     3001 aagcacgcct gacagaaaag aaaaaggcca ctcgtgagtg gccaaaattt catctctgaa
     3061 ttcagggatg atgataacaa atgcgcgtct ttcatatact cagactcgcc tgggaagaaa
     3121 gagttcagaa aattttaaaa aaaattaccg gaggtggcta aatgccgttg ttagatagct
     3181 tcacagtcga tcatacccgg atggaagcgc ctgcagttcg ggtggcgaaa acaatgaaca
     3241 ccccgcatgg cgacgcaatc accgtgttcg atctgcgctt ctgcgtgccg aacaaagaag
     3301 tgatgccaga aagagggatc cataccctgg agcacctgtt tgctggtttt atgcgtaacc
     3361 atcttaacgg taatggtgta gagattatcg atatctcgcc aatgggctgc cgcaccggtt
     3421 tttatatgag tctgattggt acgccagatg agcagcgtgt tgctgatgcc tggaaagcgg
     3481 caatggaaga cgtgctgaaa gtgcaggatc agaatcagat cccggaactg aacgtctacc
     3541 agtgtggcac ttaccagatg cactcgttgc aggaagcgca ggatattgcg cgtagcattc
     3601 tggaacgtga cgtgcgcatc aacagcaacg aagaactggc gctgccgaaa gagaagttgc
     3661 aggaactgca catttagtca gtttaagcag aagaggccgg gctggtgaaa acaccgcccg
     3721 gcttttgtta cctgtcagtc atccgatatt ccacgctatg tgcttcatca ggttgaggaa
     3781 cttccagcct tgaggccatc cgcatcacga ccgcttcagg aactgcatac tcgcgtctgg
     3841 cattttgctg cttccattgc gcccatggca cttccagata aacgattttt actcgtgcgc
     3901 cgtaagccgt aaacagactg atgagctggc tgcgcagctg gcgggtaatg ttcgttgcat
     3961 tccagatgaa cggctttttc tggcgtaaaa aaacgcgcgc ctcttctttt gcctgttgca
     4021 caatacgccc ggtggcggtt ttgtcgtccg gactggcatt gattcgccgc cgcatatcat
     4081 caaggctgat aacgtccatt ccctggcact gttcactgat atagcgatct ttgcccatac
     4141 ccggtaagcc acacagcaaa ataacctcaa aaggttcagc ctcccaggga acaaaatcag
     4201 gagaactttg ctcatgagtc aggtagtgcc agcgtgcgga atcagaaacg aacgggcgca
     4261 tttttcccca gcattgttgc tcatggcaaa acagctcgaa cagatcgatg cgttccagca
     4321 ttgattgctg atccgggctt tggcgaccaa gcagatcggc acgagcaagc aaagcgagca
     4381 gacgcgtgtc gatgcgcatt gcagccgtga gcagcaaacg ctccggttcc ggacgttcca
     4441 gtaaccatag cggtaaccca tgcagacgca ccagcgcaac aatttgctcg cgtagcacga
     4501 acggcgttgg gatatcgcgc cataaaattt gtcgggccgt cagttcgccc cgacgagcat
     4561 ggccaggcga ctgaatacgt ccgttttcct gcacggtagt gctgcgcttt tcaacatcat
     4621 gcaataacgc cgccgcccat aaaacttcct gctgttgagc ggggagttgc tggaattctg
     4681 gctgagtaac gagggcgttc agcaccattt cggtatgcac gccgacgtca ccttcaccat
     4741 gatgctccgg gtcttgtggc gtgtgatgca tctcctcaac ccagctaaaa cgctgacgta
     4801 gcgccgacca gcgtttgtcg tcggtaagct gccaaatcat gactgttctc cataatcgaa
     4861 ctcatgggcc atccgcgccc gttgccagtg tcgtttccag tggacggtgg ttttgacatg
     4921 atttttgcgc acatacttga agacgttatg cgaaaagtca gcgacggaaa acgcatcacg
     4981 attcctgctg acaatgcctt ccagcgtaca gggttgcccg gtttgcgtat cccagggatc
     5041 gaacgcgccg cgcgcattgg tcagcgccag aaaatcacgt tgccagcttt tttcatcatt
     5101 ccccggttgc gggccggaga tttcgggaac gcaggggaaa tcaaacagag cggcgtaaaa
     5161 ctgcacctct tcccagctca gccacatatc ctggcagcga acggcaaaca gataaaaatc
     5221 ctgctccagg gcgcgatatt caatcgaatg cacggcataa agattttcac caaacaactc
     5281 cagatcgccg agatcgtttt tcagcagttg ccagcgttgg cgaattttat aggtccatgc
     5341 cgactgcgta ggcgcggcat gggaacgggc aaacacgccg tagcggttaa ggcagttatt
     5401 ttcaccgtcg agtttttctg tgtgcaccaa ttgcgtgatg gcctgtaaat cctgccagta
     5461 atcggcatta atgcggtcat cgctggtggt gccgggggag aaaggataat gccaggttct
     5521 cccgtatttt cgttgggtat tcataataa
//
LOCUS       DAZGHF010000221         5510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGHF010000221 DAZGHF010000000
VERSION     DAZGHF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.222"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..258)
                     /locus_tag="ACHK3A_004748"
     CDS             complement(<1..258)
                     /locus_tag="ACHK3A_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO8742720.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYS"
     gene            complement(255..1925)
                     /gene="menD"
                     /locus_tag="ACHK3A_004749"
     CDS             complement(255..1925)
                     /gene="menD"
                     /locus_tag="ACHK3A_004749"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO8742721.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2014..3309)
                     /gene="menF"
                     /locus_tag="ACHK3A_004750"
     CDS             complement(2014..3309)
                     /gene="menF"
                     /locus_tag="ACHK3A_004750"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO8742722.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQTFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGEIAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3388..3693)
                     /gene="elaB"
                     /locus_tag="ACHK3A_004751"
     CDS             complement(3388..3693)
                     /gene="elaB"
                     /locus_tag="ACHK3A_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO8742723.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3748..4209)
                     /gene="elaA"
                     /locus_tag="ACHK3A_004752"
     CDS             complement(3748..4209)
                     /gene="elaA"
                     /locus_tag="ACHK3A_004752"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8742724.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            4274..5191
                     /gene="rbn"
                     /locus_tag="ACHK3A_004753"
     CDS             4274..5191
                     /gene="rbn"
                     /locus_tag="ACHK3A_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO8742725.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQALKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCNA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPVTELANDFTVFNV"
     gene            5379..5489
                     /locus_tag="ACHK3A_004754"
                     /pseudo
     CDS             5379..5489
                     /locus_tag="ACHK3A_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         1316 a         1514 c         1358 g         1322 t
ORIGIN      
        1 cgagtacccc accagccaga agttaaggat gttgtaacta actaaggttt tacgcagtaa
       61 gtcggtgaca tcatcaaatc catcgacgct aatcgccgcc gaaccaccgt gacctgggag
      121 atcaacatac aaccgtgagt agtcggcaaa cgcctcgccc acttcttgcc attcatggca
      181 atcgccggaa aaaccgtgca aaaacaccag ccagggtaaa cctggtttac cgtgttttgc
      241 ctgcgcgtgc aggatcataa atggcttacc tgcgccagca gttgctggag ggtttgcgca
      301 ccatcggtgt cgttaaccac catttcaatt accgtggtgg tcggcgtgcg ccaggcgtcg
      361 gcaagtgccg tttcaagttc ctgccagttt tgcggacgat gatatttcag ctcgaacatc
      421 gcggcggcgt gctcaaaatg gacgttttgc ggcatcagat agaaacgctc acgctcgttt
      481 ttcggcgttg gcaacagcga gaaaatttgc ccgccgttgt tgttcaccac aattaatacc
      541 agcggcgcgg aaacctgacg taataacgcc agcgcgttga gatcgtaaag tgctgagaga
      601 tcgcccacaa tcgccagcgt cggtttgcca ctggcccgct gaacgccagc ggcggtcgag
      661 agcagcccgt cgataccgct ggcaccacgg ttgctgtaca ccgggtaacc tgccggaagt
      721 tgcgaaagcg catcaatcag acgtaccacc aggctgttac caacaaacaa ttgaccctgt
      781 tcaggcagat agtcgctgat gcgatgcgcc agttgcgctt cgccaaacgc atcgcgacgg
      841 gcaataaccg cctgcattgc ctgttccgcc aggcgcggaa tttcaacgca ccagggctgg
      901 cgtttttctg ccggatgctg ctccagccag tcggcaatat tggcaattaa gcgacgtcca
      961 cgatggtgtg ccggatcaag tcgcccttcg atgtcatcaa caatccagta ctcttccggt
     1021 tcacagcttg cctgccattg caggagccgt ttgcccgtca ggctgcttcc cagttgcacc
     1081 acaatttgtg cctgttgcag ttcgctggtc gctttggcat tgcctaacca gagatcggca
     1141 cacggcagcg gctgtccggt ttgcgacagc acgtcgccaa tcagcggcca gccaagagtt
     1201 tgcgcccaca gtgcaacttt tttgccctct tccgcactca tgcgcccggc aaccaccacg
     1261 ccgcgctttt gccgccagaa gaaccagtcg cgctgttttt cactttccag acgaggcgct
     1321 tcacgcagcc acggtttgtc gtcctgccac cagtcgccca gccgctgttg ccagctaagc
     1381 ccggtgtcgt ccatttcgcc atacagcggt tcagcaaacg ggcagttgat atggactccg
     1441 cccgcatgaa gcgtaccgag agcatggtcg atggtagaaa ccagccaacg tgcaggaata
     1501 tcctgagtcg ggcgcggcag tgaaatactg tgcgtggggt gagaggcgaa cattcccggc
     1561 tgacgaatcg cctgattcgc gccgcagtca attagctccg gcgggcgatc ggcggttaac
     1621 aggatcagtt tttctccggt taacccagct tcaatcagtg ccggatagag atttgctacc
     1681 gccgtgccgg aggtcacaat caccgccacc ggctgcttgc tgactttcgc cagccccagc
     1741 gccaaatgcc ccagtccacg ctcatcgaaa tgggtgtggt gaatgaatgc ggaattctcc
     1801 gccgccgcta acgttaacgg tgtagaacgc gagccagggg cgatacagat gtgtctgacg
     1861 ccgtgacgcg ttaatgcttc cagaatgacc gccgcccagc gtcggttaaa tgcgcttact
     1921 gacatgagtt tgtccggtat caatattgcg gctaagtata aggagctaca aaaatagatt
     1981 attgatatga atcggtaatg atgcgactca ttactattcc atttgtaata aagtacgcag
     2041 ccctgccgct ttgttatcga tttcctgcca ctcttgctcg gggtcagaac cacggacaat
     2101 gcccgcgcca gcatatagcc gcacgacatt gccactgatt tttgctgagc gcagggaaac
     2161 gcagaattcg ctttgttgta aagagagata gcccgcagaa ccggcgtacc attctcgggt
     2221 gaacggttcg tgacgggcga taaactgtcg cgccagatcg cgcggtaagc cagcaactgc
     2281 tgccgttggc tgcaactgat gtaaacagat cacatcatcc gctttgttga gtgcagtcca
     2341 gatacagcgg cgaagatgct gcactttacg cagacgcagt acctgcggcg gtaaaacatc
     2401 caacgtctgg gtatcggcct gcaatcgttg gcagatatcc tccaccacca acatgttctc
     2461 gcgctggttt ttatcatccg ccatcagcca ctcacctaac tgctgcgcct gcttatcatc
     2521 aggatgattt gctaccgttc ccgccagcgc ttcagtacgc agcgctttgt cacgccgccg
     2581 ccataaccgt tccggtgaag agccaagaaa agcaatttcg ccatcaaagg ccatgtaaaa
     2641 atggtagcaa ttcagattca gtcgacgact ggcagccatc atcgccgccg cattgaccgg
     2701 acttgcgaaa tgcaggtcag ttgcccgagc aagcaccact ttgtcgagct caccttcggc
     2761 gatggttttc gttgccagtt cgattaattg cgtccagccc gttttgtccg gccagtgttg
     2821 ttctcgggtg gtggttaaat gtaacccagg caagggttta atactcacca gtgtggcgat
     2881 aaattctttt gccttaatcg catcatgctg aagggagctt tcgctgaata gcgtcagccg
     2941 cagcgtggct ttaccgccac agcgtcgcca ttcgaggcgt ggtaaaagta aattgccctg
     3001 cgacgggtca aacgcattta gcccccagat gcgtaagtcg gcgtgttccg ggtgctgacg
     3061 aagaaacgtt tgtgcctggt ccaacgacgt aaaacgggta atcgcgccca ggacggcagc
     3121 ttcttcatca ccattacgtt gttgccagta gaattgcggg tacgtttgct gactggccag
     3181 ccagctcaag gcatcaaaag cgtctttgag agggaaagga atatcaataa cccgaatgcc
     3241 gggtgtcgcc ggaatctctt gcgacaaatg gcgcagtaga ttttccagcg ccgtagtaag
     3301 tgattgcacg cggacctctc cctgttaaaa gcctcacatt atacggggta cgacaaaaaa
     3361 aatgcagtac cccggtgtag ggaggtttta acggcgtgcc agcaacagtc ctagtaccag
     3421 cccaacggcc gcgcccacac caattccttg ccagggtttt tcgtggacgt agtcatcagc
     3481 acgataaaca gcctgcttcg cccgatagta ataactgtct gaagcctggc taacccgttt
     3541 tttcacatca tccagcgctt tttctgcacg cgctttcagc tcaacatatt tctgatcggc
     3601 gggatcgcca gaggagcgga gcacctcttc cagtgtttca ctaagcagcg tcaggtcatc
     3661 atcaatacgt gtatcaccaa actgattaga cattctcgtt ctccatttgt gtaaaacctg
     3721 tcactaacta tagacaaggg ttattgatta cgcctgaatt acctcgcgtg ccatcccaat
     3781 gtgtgggata ccatcttctt cataaatatc ggtcacaggg atgaaaccaa attgcccata
     3841 aaagttttgt aaatgcgcct gcgcccccag ataaatcggc ttctccggcc agcggcgagt
     3901 acacgattcc agcgccttac tcatcaattg ctgaccgatt ttttcaccgc gtaaggcttc
     3961 gctgacaatc acccgaccaa tcacaacggg ttgcagttcg tcgtcacttt tcagaatcct
     4021 cgcatacgcc accagtgtcc cgttatgcca accgagaatg tggcgatttt ctccctccag
     4081 atcgtcacca tcgatatcct ggtaaggaca gttttgttcc actacaaata ccgcacaacg
     4141 cagttgtaat aaggcataaa gctgagaaac ggataaatca gaatgatgca gatcttgcca
     4201 gtcgatcatg gaaggctcct ttgtgctgtt tgctttatac tagccggaat attttcagca
     4261 gcgtgatgaa ctcatggaat taattttttt aggtacttca gccggtgtgc caacccgtac
     4321 gcgcaatgtc acggcaatat tacttaacct gcaacatccg acccagagcg gtctttggtt
     4381 gtttgactgc ggtgaaggca cccagcatca gctactgcat accgccttta accctggaaa
     4441 actggacaag atttttatca gtcaccttca tggcgatcat ctctttggtt tacccggctt
     4501 gctgtgcagt cgttctatgt caggcattat ccaaccctta acgatttatg gtccgcacgg
     4561 tatccgtgaa tttgtggaga ccgcgctgcg gattagcggc tcatggaccg attatccgct
     4621 ggaaattgtc gaaattggcg ctggcgaaat tttcgatgat ggcctgcgca aagtgaccgc
     4681 ttatccgctg gaacacccac tggaatgcta tggctatcgt attgaagaac atgataaacc
     4741 gggtgcatta aatgctcagg cattaaaagc tgctggcgtg ccgcctggcc cactgtttca
     4801 ggcattaaaa gcgggcaaaa caatcacgct ggaagacgga aggcagatta acggcgcaga
     4861 ttacttagcc gctccagtgc caggtaaagc gctcgctatt ttcggcgata ccggcccctg
     4921 caatgccgca cttgacctgg ctaaaggtgt cgatgtcatg gtgcacgaag cgacgctgga
     4981 tataaccatg gaagccaaag ccaatagtcg cggtcatagc tctacacgcc aggctgcgac
     5041 actggcccgt gaggctggag tcggtaagct aatcattacc cacgtcagct cgcgctatga
     5101 tgacaaaggg tgtcagcacc tgttacgtga atgcaggtca attttcccgg tgactgaact
     5161 agcgaatgat ttcaccgtgt ttaacgttta actctgctta ttattaaaaa cagggcgaaa
     5221 cttgccctgt tatcgcaacc cgcgctacat atatccaccc acacgggtat attgagcgat
     5281 ttttgatatt tattcatctc ctcacgcact atattcagag caataagttg cctcttttta
     5341 tcttcattca gcacaacctt tgcaactaaa aggagcctat gatggttaca gttgtcagca
     5401 attattgtca attatctcaa acccaactct gtcagacatt tgcagaaaaa tttactgtga
     5461 ccgaggaatt actgcagtct ttaaaaaaac acttataact cattacaaat
//
LOCUS       DAZGHF010000222         5431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGHF010000222 DAZGHF010000000
VERSION     DAZGHF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.223"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(36..773)
                     /gene="bamD"
                     /locus_tag="ACHK3A_004755"
     CDS             complement(36..773)
                     /gene="bamD"
                     /locus_tag="ACHK3A_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO8742726.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQLNAQAEKVAKIIAANSSNT"
     gene            908..1888
                     /gene="rluD"
                     /locus_tag="ACHK3A_004756"
     CDS             908..1888
                     /gene="rluD"
                     /locus_tag="ACHK3A_004756"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO8742727.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1885..2616
                     /gene="yfiH"
                     /locus_tag="ACHK3A_004757"
     CDS             1885..2616
                     /gene="yfiH"
                     /locus_tag="ACHK3A_004757"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO8742728.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCSGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYLLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2746..5319
                     /gene="clpB"
                     /locus_tag="ACHK3A_004758"
     CDS             2746..5319
                     /gene="clpB"
                     /locus_tag="ACHK3A_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO8742729.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1338 a         1297 c         1476 g         1320 t
ORIGIN      
        1 gctcaagggc tgccgttttg tgtttcaggt ttctgttatg tattgctgct gtttgcggcg
       61 atgattttcg ctactttttc agcttgcgca ttcaactgca tctgacggta tgcattttcc
      121 atcagcggca gcgcatcacg cgtagcctgg gtatccgggt agtcgcgcaa catgccttct
      181 acgcggttaa cgacggcaac ccatgcacca cgttctgtat agtactcggc aacggagtat
      241 tcatatttcg ccagacgatc tttcaggaat accagacgtt tggtggcatc ggtggtgtac
      301 tgactgttcg gatagccgcg caccagtttg gaaaagtcac taaacgcagc tcgtgcatgt
      361 tgaggatcgc gatcgctacg gtcaacgcca aagaaccctt gcagcgcact gtcatccagc
      421 gccatattgg tcaggccacg catgtacatg acataatcga tattcggatg ggtcgggtta
      481 aggcgaataa aacgatcgat ggcagcctgt gctaacggca aatcggcgtt tttatagtag
      541 gcgtagatga gatccagctg cacctgctgc gaatacggac caaacggata gcgattatct
      601 aacgcttcca gttgcgttat tgcctgtctc cagttaccgt cctgcagctt ttgttgtgca
      661 gtcgcgtaaa tttcatttgg cggattatca ggtacttctt cctttgaccc cgagcaaccc
      721 gccaaaaaca ggcttagtgt ggcggctgcc accagatatt tcatgcgcgt catgacgttt
      781 tgactttcct caaaatgtaa tacgggagat tctctgttcc tgctcccggt taagaccagc
      841 tacaatagca cactatatta aacggcaaag ccgtaaaacc ccaacgataa acgaagaagc
      901 agtatatatg gcacaacgag tacagctcac tgcaacggtg tccgaaaacc aactcggtca
      961 acgcttagat caggctttgg ccgaaatgtt cccggattat tcacgttcgc gaataaaaga
     1021 atggatcctc gaccagcgcg tgctggttaa cggcaaagtt tgtgataagc cgaaagaaaa
     1081 agtattgggt ggcgagcagg tggccatcaa cgctgagatt gaagaagaag cgcgttttga
     1141 accgcaggat atcccgctgg atatcgtcta tgaagatgaa gatatcatcg tcattaataa
     1201 accacgcgac ctggtggtac atcctggcgc gggtaacccg gatggtacgg tactgaatgc
     1261 gttgcttcat tattatccgc ccattgccga tgtaccgcgt gcgggcatcg tccatcgtct
     1321 ggataaagac accaccggcc tgatggttgt ggcaaaaacc gttccggctc agacgcgttt
     1381 agtcgaatct ttgcaacggc gtgaaattac tcgtgagtat gaagcggtgg caattggtca
     1441 tatgaccgct ggcggtacag tagatgaacc aatcagccgc cacccgacca aacgtactca
     1501 catggcggtg catccgatgg gtaaaccggc ggtcactcac tatcgcatca tggaacactt
     1561 ccgtgtgcac acgcgtctgc ggttgcgtct ggaaaccgga cgtacgcacc agatccgcgt
     1621 gcatatggcc catatcactc atccgctggt gggcgatccg gtttatggtg gccgtccgcg
     1681 tccgccaaaa ggtgcttcgg aagcgtttat ctccacgctg cgtaagtttg accgccaggc
     1741 gcttcatgca accatgctgc gtctttatca cccgatctcc ggtatcgaaa tggaatggca
     1801 tgcgcctatt ccagaagata tggtggagct gattgaggtg atgcgcgccg atttcgaaga
     1861 gcataaggat gaagtggact ggttatgagt aagctgattg tcccgcagtg gccgctgcca
     1921 aaaggtgttg cggcctgtag ctccactcgt atcggcggcg tgagcttgcc cccgtatgat
     1981 tcgctcaacc tcggtgctca ttgtggcgat aacccggatc acgttgaaga gaatcgtaag
     2041 cgactttttg ctgcgggcaa tttgccttca aaaccggtct ggcttgagca ggtacacggc
     2101 aaagatgtgc ttaagcttac tggcgaacct tatgcctcaa aacgagcaga tgcctcttat
     2161 agcaacacgc cgggcacggt ttgcgcagtg atgactgccg actgcctccc cgtgctgttt
     2221 tgcaatcgag cgggaacgga agtcgccgcc gctcatgctg gctggcgtgg actgtgctca
     2281 ggcgtgctgg aagagacggt ttcctgtttt gctgataatc cggaaaatat tctcgcctgg
     2341 ttagggccgg caattggtcc acgcgcgttt gaagtggggg cggaggttcg cgaggcgttt
     2401 atggcagtag acgccaaagc gagtacagct ttcattcagc atggtgataa gtatctggcg
     2461 gatatttatc tccttgcccg gcagcgtctg gcgaacgtcg gtgtcgaaca aattttcggc
     2521 ggtgaccgtt gtacatatac ggaaaatgag actttcttct cttatcgtcg cgacaagacc
     2581 accggtcgta tggcaagttt catttggctg atataaccta aagaatcaag acgatccggt
     2641 acgcgtgatt ttcttttcac attaatctgg tcaataacct tgaataattg agggatgacc
     2701 tcatttaatc tccagtagca actttgatcc gttatgggag gagttatgcg tctggatcgt
     2761 cttactaata aattccagct tgctcttgcc gatgcccaat cacttgcact cgggcacgac
     2821 aaccaattca tcgaaccact tcatttaatg agcgccctgc tgaatcagga agggggttcg
     2881 gttagtcctt tattaacatc cgctggcatt aatgctggcc agttgcgcac agatatcaat
     2941 caggcattaa atcgtttacc acaggttgaa ggtactggtg gtgatgtcca gccatcacag
     3001 gatctggtgc gcgttcttaa tctttgcgac aagctggcgc aaaaacgtgg tgataacttt
     3061 atctcgtcag aactgttcgt tctggcggca cttgagtctc gcggcacgct ggccgacatc
     3121 ctgaaagcag caggggcgac caccgccaac attactcaag cgattgaaca aatgcgtgga
     3181 ggtgaaagcg tgaacgatca aggtgctgaa gaccaacgtc aggctttgaa aaaatatacc
     3241 atcgacctta ccgaacgagc cgaacagggc aaactcgatc cggtgattgg tcgtgatgaa
     3301 gaaattcgcc gtaccattca ggtgctgcaa cgtcgtacta aaaataaccc ggtactgatt
     3361 ggtgaacccg gtgtcggtaa aactgccatc gttgaaggtc tggcgcagcg tattatcaac
     3421 ggcgaagtgc cggaagggtt gaaaggccgc cgcgtactgg cgctggatat gggcgcgctg
     3481 gtggctgggg cgaaatatcg cggtgagttt gaagaacgtt taaaaggcgt gcttaacgat
     3541 cttgccaaac aggaaggcaa cgtcatcctg tttatcgacg aattacatac catggtcggt
     3601 gcgggtaaag ccgatggcgc aatggacgcc ggaaacatgc tgaaaccggc gctggcgcgt
     3661 ggtgaattgc actgcgtagg tgccacgacg cttgacgaat atcgccagta catcgaaaaa
     3721 gatgctgcgc tggagcgtcg tttccagaaa gtgtttgttg ccgagccttc tgttgaagac
     3781 accattgcta ttctgcgtgg cttaaaagaa cgttacgaac tgcaccacca tgtgcaaatt
     3841 actgacccgg caattgttgc agcggcgacg ttgtctcatc gctacattgc tgaccgtcag
     3901 ctgccagata aagccatcga cctgatcgat gaggcagcat ccagcattcg tatgcagatt
     3961 gactcaaaac cagaagaact cgaccgactc gatcgccgta tcattcagct caaactggaa
     4021 caacaggcgt taatgaaaga gtctgatgaa gccagtaaaa aacgcctgga tatgctcaac
     4081 gaagaactga gcgacaaaga acgtcagtac tccgagttag aagaagagtg gaaagcagag
     4141 aaagcatcgc tttctggtac acagaccatt aaagcggaac tggaacaggc gaaaatcgct
     4201 attgaacagg ctcgccgtgt gggggacctg gcgcggatgt ctgaactgca atacggcaaa
     4261 atcccggaac tggaaaagca actggaagcc gcaacgcagc tcgaaggcaa aactatgcgt
     4321 ctgttgcgta ataaagtgac cgatgccgaa attgctgaag tgctggcgcg ttggacgggg
     4381 attccggttt ctcgcatgat ggaaagcgag cgcgaaaaac tgctgcgtat ggagcaagaa
     4441 ctgcaccatc gcgttattgg tcagaacgaa gcggttgatg cggtatctaa cgctattcgt
     4501 cgtagccgtg cggggctggc ggatccaaat cgcccgattg gttcattcct gttcctcggc
     4561 ccaaccggtg tggggaaaac tgagctttgt aaggcgctag cgaactttat gtttgatagc
     4621 gacgaggcga tggtccgtat cgatatgtcc gagtttatgg agaaacactc ggtgtctcgt
     4681 ctggttggtg cgcctccggg atatgtcggt tatgaagaag gtggctacct gaccgaagct
     4741 gtgcgtcgtc gtccgtattc cgtcatcctg ctggatgaag tggaaaaagc gcatccggat
     4801 gtcttcaaca ttctgttgca ggtactggat gatgggcgtc tgactgacgg gcaggggaga
     4861 acggtcgact tccgtaatac ggttgtcatt atgacctcta acctcggttc cgatttgatt
     4921 caggaacgct tcggtgaact ggattatgcg cacatgaaag agctggtgct cggtgtggta
     4981 agccataact tccgtccgga attcattaac cgtatcgatg aagtggtggt cttccatccg
     5041 ctgggtgaac agcacattgc gtcgattgcg cagattcagt tgaaacgtct gtacaaacgt
     5101 ctggaagaac gtggttatga aatccacatt tctgacgagg cgctgaaact gctgagcgag
     5161 aacggttacg atccggtcta tggtgcacgt ccgctgaaac gtgctatcca gcagcagatc
     5221 gaaaacccgc tggcacagca aatactgtct ggtgaattgg ttccgggtaa agttattcgc
     5281 ctggaagtta atgaagaccg gattgtcgcc gtccagtaat gataaaacga gcccttcggg
     5341 gctcgttttt gtctataaat tagacggaaa agactatgtt taagatgttt tgcctgaaaa
     5401 atgagcgaac gataaagttt ttatattttt t
//
LOCUS       DAZGHF010000223         5386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGHF010000223 DAZGHF010000000
VERSION     DAZGHF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.224"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..266)
                     /locus_tag="ACHK3A_004759"
     CDS             complement(<1..266)
                     /locus_tag="ACHK3A_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO8742730.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIV"
     gene            complement(418..1158)
                     /locus_tag="ACHK3A_004760"
     CDS             complement(418..1158)
                     /locus_tag="ACHK3A_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO8742731.1"
                     /translation="MNKVIAVSALAMAGMFSTQALADESKTGFYVTGKAGASVMSLAD
                     QRFLSGDGEETSKYKGGDGHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTG
                     ISTWDYGYGSSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSSVS
                     YKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            1382..1567
                     /locus_tag="ACHK3A_004761"
                     /pseudo
     CDS             1382..1567
                     /locus_tag="ACHK3A_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            1796..4105
                     /locus_tag="ACHK3A_004762"
     CDS             1796..4105
                     /locus_tag="ACHK3A_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HFO8742732.1"
                     /translation="MELWIFIVAIYLIYILFFKKKKTKSAGSYTSAPVKAPPKEWLAN
                     NKKNQSVMQNEDSEDDNLATFTLSGGRNVEYRITATQRQNFSKTTGALARWIFPGEMI
                     TVSGIEVTGGHFYLGQRMKPAGHNSGGYYDGNNEASLVDDTLKIYPTPYIYEDSSLGY
                     WPSFSSLSPEARGAYLSWLASDRCDTSCPIGYVFIYLYGLERRALVDSRDNNISDDEF
                     YSLFKEICRLRSVFNENRSFRNYSSQLLEAMSILRPNENLAAALNDDSEFSNSMQFRL
                     ILAKTVDTGAPVPADLALTWVTNHAEYSLRTPARRCAKEFAALFKRRYTLKYGEGMVV
                     KPNKARLRLDYTPASPSLRGIRLPVPDLPDPGALKGPVQKLMAIADICTGELDAYSRY
                     LGRKGTSANDTAAILLLPSEIVNESAEKILSTFKHWADDAIRSKGGIVSVADYWSHMN
                     ATCPAKINKKEADLMQAFAQKMGYCLAPDPYHHHVKADVDGVLVLFPAGERGRFSPYP
                     EFITAVLTLRLGSVVALIDNSLDQAEQKVLENAINNNASFSDDEKRSLHAYLTWQLHT
                     PANMTGMKSRIELLGAAEKSAVGKVIVSVACSDGRITPAEIKQLEKIYTSLGLDPSSV
                     SSNIHQHSATETALVSSVPANQSTAGFTLDANVLARHESATDDVRKLLNSIFTEEEPE
                     QPESAPASLTGEGGLDSAHSQLYRRLLEKEQWSRKEATELCSNWNLMLGGALEVINDW
                     SYAVVDAPVLEDADDHIWIDLEIAKELEG"
     gene            4109..4510
                     /locus_tag="ACHK3A_004763"
                     /pseudo
     CDS             4109..4510
                     /locus_tag="ACHK3A_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
     gene            complement(4481..4689)
                     /locus_tag="ACHK3A_004764"
                     /pseudo
     CDS             complement(4481..4689)
                     /locus_tag="ACHK3A_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4724..4858)
                     /locus_tag="ACHK3A_004765"
     CDS             complement(4724..4858)
                     /locus_tag="ACHK3A_004765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742733.1"
                     /translation="MSEIKIIGIELAKTNFYPFNINDYGKTVGKIKFSRSNLLNLLVQ
                     "
     gene            complement(5082..>5386)
                     /locus_tag="ACHK3A_004766"
     CDS             complement(5082..>5386)
                     /locus_tag="ACHK3A_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO8742734.1"
                     /translation="FDKGSLLYTNFYNAEDHKKVSEAILKLKHPWLLTYDNALEINEL
                     YNNCARYTYPLNYSAATKRVGTELLITQDGIHIPNNLGITRVINKEKVMSTDEVSI"
BASE COUNT         1551 a         1184 c         1177 g         1474 t
ORIGIN      
        1 acaataaaac tgttgttgat tttaccgtac gtcatacccg caggcgcata cagtgcacaa
       61 cagaacagaa ataacagagc tgtaatggat gtaagggtat ttctgtgtgc aaggagcaga
      121 agaaggaaca gcagcaacac attcccggtg gcattcttct cagtgtatcc gcatgcaatt
      181 gtggttatga cagaaataac aaaaaagaat aaaaacaata aaatccgtgg tgtgttgtcc
      241 ggacaaaaca gttttctgat attcatcgga gtatatcgac aacattattg tgaagagaat
      301 aaaaatcaga agtctcctgt gaaacagata acaaacaacc ctgcagtata attactgcag
      361 ggagtcttcg tttaattaca gaagatgggt aattattatc tttcaggagc agttttgtca
      421 gaaattataa gtcacaccaa gcatgatgtc atgactttta acatcaactt ctgatttata
      481 tttatcgccc cactcgtcct tgtaactcac actgctgtca cctgcatcaa gatagcgata
      541 gctgaggtcc agagcgatat ccggggttac gtcatagcga acacccgcgc caaggctcca
      601 tgcgaagttg tcagcagagc ctgaacgtga caacgattcg cgaccactgc ttccgtaccc
      661 ataatcccag gtactgatac cggttgtttt ctggtgaatt ctggcgtagc caatccctgc
      721 agatacccat ggtgtgaatg cactgtcatt ccggaagtca tagtacgcat tcagcatcag
      781 tgtgttgact gacacctcat tcttcaggtc atcacgccag taaccgcctg accagctgtc
      841 tttatctacg ttatacttcg aatcagcttt tccacgagcg taaaactcca gttccgtacg
      901 aaccggaata ctgaactgcg ggtaaaaatc ataaccggcc gcgataccgc cactgaatac
      961 cgtatcatgg ccatcaccgc ctttatattt tgatgtttcc tctccatcac ccgacaggaa
     1021 acgctggtct gcaagtgaca taacagaagc acctgcttta ccggtcacat aaaagcctgt
     1081 tttgctctca tcagccagag cctgggtcga aaacatgcct gccatggcaa gcgctgaaac
     1141 cgcaataacc ttattcattt caatcatctc cgctatcact ttccccgaac gataaatggg
     1201 gttgctcatt ctaaaaccat gcaaaagaca gggtaaaagg aatgttttcg atcgaatcga
     1261 tcgataaaat cgatcgtgcc cgataaagtt ttcttatcgt ccccataaaa atggtaaggg
     1321 ctggtttaag aggtaatcgc agcggcatgg aagtattaag accaaaacag cacgagataa
     1381 agcggctgaa cgtcagatga gccacatgga acgtaattca ttaagggcgg cttatatcca
     1441 taaaacagaa cacctggaat agcgtcggtt gatgctacag tggtgggggg attttctgga
     1501 tacaaactcg aaagaaaata ccagcccctt tgagtatgcg aagattaaca atccattaaa
     1561 atattaagca ctccgggcaa tctgcccgga gtgctttagc tggcataacg ggctactgat
     1621 ctcctctggt tacagataac gacttcagcc ctctgaaaga gaaaagcgga agctcaggaa
     1681 ggttgtgatt tgcttttaac ctttttaatt tagaagtatt attttgagat taatccactt
     1741 cccatcgtca tcctttttgg tgtgcgtgaa aagctatttt taaggttact gcctcatgga
     1801 gttatggata tttatagtag ctatttattt gatttatatt ctttttttca aaaagaagaa
     1861 aactaagtcc gcgggctctt atacttccgc tcctgtgaaa gcaccaccta aagaatggct
     1921 tgctaacaac aaaaaaaatc agtctgtaat gcaaaacgag gacagcgaag atgacaatct
     1981 ggctacgttt acactaagtg gcgggcggaa tgtcgaatac aggataacag ccactcaacg
     2041 ccagaatttc tcaaaaacta ctggtgcttt ggctcgttgg atttttccgg gagagatgat
     2101 cacagtcagt gggatagagg ttaccggcgg tcacttctat cttgggcagc gtatgaaacc
     2161 tgccgggcat aactcaggcg gttattatga tggcaataac gaggcatcgc tagttgacga
     2221 cacgcttaaa atatacccca caccttacat ttacgaagat agttcattag ggtattggcc
     2281 cagtttttcc tcactttcac ctgaagctcg tggtgcgtac ctgagttggc ttgccagcga
     2341 cagatgcgac acttcctgcc caattggtta cgttttcatt tatctgtatg gtctggaaag
     2401 aagggcgctt gttgacagca gggacaacaa tatatccgac gatgagttct attctctttt
     2461 caaagagatc tgccgtctca gatcggtttt caatgaaaac cgatctttcc ggaattactc
     2521 atcccagtta cttgaagcga tgtccattct tcgtccgaac gaaaatcttg cagcagctct
     2581 caacgatgat tcggagttca gcaatagcat gcagttcagg ttaatcctgg caaagactgt
     2641 tgatacagga gccccggttc cagcggatct ggctctgacg tgggtgacaa accatgctga
     2701 atactcatta cgaacccccg cccgccgctg tgcgaaagaa tttgctgcac tatttaaacg
     2761 gcgttacacc cttaaatacg gtgaagggat ggtagtgaag ccgaataaag cgcgtctgcg
     2821 tttagattac acgcctgcaa gtcccagttt gcgaggtatt cggcttcctg ttcccgactt
     2881 gcctgatcca ggtgcattga aaggcccagt tcagaagctt atggccattg ccgatatatg
     2941 cacaggtgaa ctggatgctt acagtcgtta ccttggtaga aaaggtactt cagcaaatga
     3001 taccgctgca attttgttgc tgccttcaga aatcgttaac gaaagcgcag aaaaaatatt
     3061 aagtacattc aaacactggg ctgatgacgc aatccgcagt aagggtggga tagtttcagt
     3121 tgctgactac tggtcacata tgaatgccac ctgtccggcg aagatcaata aaaaagaggc
     3181 cgacttaatg caagcctttg cacagaaaat gggatactgt ctggctcctg acccctatca
     3241 ccaccacgta aaggcagatg tcgatggtgt acttgtctta ttccctgctg gtgaaagagg
     3301 acgtttctca ccttatcctg aatttatcac agctgtattg acgctccgat taggttcggt
     3361 agtcgccttg attgacaatt ctctcgatca agccgaacag aaagtgcttg agaatgccat
     3421 caacaacaat gccagcttca gcgatgatga aaagcgctct ctgcacgcat atttaacgtg
     3481 gcagcttcat actccggcga atatgacggg aatgaaaagc cggattgaat tactcggtgc
     3541 agcggaaaaa tcagctgtgg gtaaggttat cgttagtgtc gcttgctctg acggcagaat
     3601 tacgcctgcc gaaatcaaac agcttgagaa gatttataca agcctgggac tggatccctc
     3661 atccgtatca agcaatatcc atcagcattc cgcaactgaa actgctctcg tttcatctgt
     3721 accagctaat cagtcgacgg cagggtttac gctggatgcc aacgtacttg cccgccacga
     3781 atctgccact gatgatgttc gtaaattact gaactcaatt ttcactgaag aagaaccaga
     3841 acaaccagaa agcgctccag cctcattaac cggggaaggg ggacttgact ccgctcatag
     3901 ccaactttat cgcaggttgc tggagaaaga acaatggtcc agaaaagagg cgacagagtt
     3961 gtgtagtaac tggaacctga tgctcggcgg tgcgcttgag gtgattaacg attggtccta
     4021 tgcggtggtt gatgcaccag tgctggaaga tgctgatgat cacatttgga ttgacctgga
     4081 aatagccaaa gaattagagg gttagtgaat gtctgtaaca cgtataagag tgaaagagcg
     4141 cgacgctatc attcagtcac ttaagtcggg agtaacgcca agaataggta ttcagcatat
     4201 ccaggttggc cgcgtgaatg agataaccgc cctccatcag gatattgaac ggattgctga
     4261 tggcggggca agcttcagac ttatcattgg tgagtacggt tccgggaaaa cattcttttt
     4321 aagtgttgta cggtctattg cgttagagaa gaagctggtc acggtcagtg cagacctttc
     4381 tcccgacaga cgtatccact catcgggagg tcaggctcgt aatctctact cagagcttat
     4441 gaaaaatatg tccaccagaa acaaaccaga tggcaatgca ttacttagcg ttgtggaaag
     4501 gtttgttaca gtcaaggcag tcaccaaact cgataataaa gcggctcatt tccatacgtc
     4561 tgtccttaag gggcattgtt catttctgtg accaagccgc accacctttt ttactgattc
     4621 gtctgtcggg aacactttgc gtttcttgat gacataacgg atcacactat tcaacgactc
     4681 tatggcattt tgcacatact tccatagcaa ctgtcatcgg tagctactga accagcaagt
     4741 taagtaagtt actacgtgaa aattttattt ttccaacggt ttttccataa tcattaatat
     4801 taaaaggata aaaatttgtt ttagccagct cgataccaat gatttttatt tcagacatga
     4861 tatccttctt acatttcatt gattaaagtt tatccatcat ggtatgatct gcttctaccg
     4921 tgaaggaggg gctgacactc aatggaccaa aaactcacgt taaacaattg tagcaaagga
     4981 aagttaaagg atcctgcgag gatcctttga taggtgttaa aattgcattc ttattaccat
     5041 ataagatcag ttatctctca gtagctgtga gcatattaaa tttatattga cacttcatcc
     5101 gtactcatta ctttttcctt attgataact cttgtaattc ctaaattatt tggaatatgt
     5161 atgccatctt gagttataag taactctgtt ccaactcgtt tcgttgcagc agaataattc
     5221 aatgggtacg tatagcgtgc acaattatta tataattcat taatttctaa tgcattatca
     5281 taagttaaaa gccatggatg ctttagtttt aaaatagctt cggacacttt tttatgatct
     5341 tcagcattat aaaagttagt gtagagcaat gagcctttat caaagt
//
LOCUS       DAZGHF010000224         5382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGHF010000224 DAZGHF010000000
VERSION     DAZGHF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.225"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..255)
                     /locus_tag="ACHK3A_004767"
     CDS             complement(<1..255)
                     /locus_tag="ACHK3A_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8742735.1"
                     /translation="MTYEVIITSEAEQQIINLHRYITEKAGSAIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGNKVFIA"
     gene            complement(261..518)
                     /locus_tag="ACHK3A_004768"
     CDS             complement(261..518)
                     /locus_tag="ACHK3A_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO8742736.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRAGK"
     gene            654..932
                     /locus_tag="ACHK3A_004769"
     CDS             654..932
                     /locus_tag="ACHK3A_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742737.1"
                     /translation="MAHDTRFYNSDNSAAPASRHGRRSHAFKSDWLQHPPCTEEQAEW
                     LIQCYRRRGYEFEKALSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            934..1983
                     /locus_tag="ACHK3A_004770"
     CDS             934..1983
                     /locus_tag="ACHK3A_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8742738.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLAAWMIDVIRHAMNDTQERE
                     LSLAELCWWAVCHQVADALPESVLRRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLKFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            1996..2370
                     /locus_tag="ACHK3A_004771"
     CDS             1996..2370
                     /locus_tag="ACHK3A_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO8742739.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKSGERYRRAVALIVRQQ
                     QLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            2367..3188
                     /locus_tag="ACHK3A_004772"
     CDS             2367..3188
                     /locus_tag="ACHK3A_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO8742740.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            3416..3613
                     /locus_tag="ACHK3A_004773"
     CDS             3416..3613
                     /locus_tag="ACHK3A_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO8742741.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            3764..4816
                     /locus_tag="ACHK3A_004774"
     CDS             3764..4816
                     /locus_tag="ACHK3A_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO8742742.1"
                     /translation="MLSTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELES
                     ERFLQTVKEIRDDFCR"
     gene            4905..>5382
                     /locus_tag="ACHK3A_004775"
     CDS             4905..>5382
                     /locus_tag="ACHK3A_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8742743.1"
                     /translation="MAVVCSVILVCSPINIFIEKDKLSLVPGMVILVTRNIRELFRTY
                     TDRVKVADINHDIVSQYLEGGRRRMSSPQACFPHYLTSGIHQPVLAEALITNHGFPSA
                     PSGDLSTMTGLSCLSLFANDESLSSFLSRCLSNTSDKVRAIIQSDISVNWKLGVIAL"
BASE COUNT         1362 a         1239 c         1477 g         1304 t
ORIGIN      
        1 agcgataaag actttattgc catcaacggc aaaagcaatg atcgttctgt ggcggaagtg
       61 agttacccgc attccctgtc gaatatcatc gcgtttattg ccccgatgcg ggaatgtgga
      121 aaaaccatca agataatcaa gaagcgcgtt ggcataattg tcagcaatgg cgctccctgc
      181 cttctctgtt atatatctgt gcaggttgat tatctgctgt tcggcctcag aagtaatgat
      241 gacttcatac gtcatgcaga ttacttcccg gctcgaatcg cggcgcgaac ctgtgaaatg
      301 gagcgtccgt tgtttgggtt ttcgcggata gaatcgagag atggggcggc tgaatgcgtt
      361 aaccacgctt cgattgcttt atcgcgctca ttcagtgcgc gaagcccttc acgaatgacc
      421 tcactttctg aagcataggc accggaagcc acacgggcgc gcaccatgtc agccatctcg
      481 ttagttaatg taatgctgaa ttgttgggtt gtacgcatgg taaacctcac ggagtaggat
      541 agaacactat tcgatgatag cacactgcct gttgacgaca ccagaaatca gatacaatat
      601 tgccgcacgc cagcctgaac aactggcacc tgctgcgcca gcagagacaa ccgatggcgc
      661 acgataccag attttacaat tcggataact ccgccgcccc tgccagcagg cacgggcggc
      721 gttctcatgc attcaaatct gactggttac agcatccccc atgcactgaa gaacaggccg
      781 aatggctgat ccagtgttac cgcaggcgcg gatatgagtt tgagaaagcc ctcagcttcg
      841 atcgtcgtca ctggataatc tccgtcaggc tcccttattc cgaacgccca ccgcgtccgt
      901 cccgcacatt ccagcagcgc atctggaggt aacgtgcggg tattacttcg acctgttctg
      961 gtaccggaac tcgggatggt gctccttaag ccgggccgtg aatcaatgtc agcattccat
     1021 aacggcagaa tattggtgga accggaaccg aaaaacatgc gcggtctgcc gtccggagtc
     1081 gttcctgccg ttcgacagcc gctggcagag gataaaacat tactgccatt tttcagcaac
     1141 gaacgggtga ttcgtgctgc gggtggcgct ggtgcactgt ctgactggtt attacgtcac
     1201 gtgaaatcct gccagtggcc acacggtgat tatcatcaca gcgaaaccgt cattcaccgt
     1261 tacggtaccg gcgcgatggt gttgtgctgg cactgtgaca accagctgcg cgaccagaca
     1321 tcagaatcac tcgatcaact tgctcagcag aatctggcag cctggatgat tgatgtcatc
     1381 cgtcacgcaa tgaatgacac acaggagcgg gaactgtcgc tggcagagtt atgctggtgg
     1441 gctgtttgtc atcaggtggc tgatgcgctt ccggagtctg tattgcgtcg ttcgctggga
     1501 ttaccagcgg aaaaaatccg ctccgtatac cgtgagagtg acatcgtacc gggagaacag
     1561 acagccatca gcatactgaa gcagcgcaca aaaaatattg cgctgccact tcacgtccac
     1621 cagcaacaaa atccaccaca gaaaaaaacg gttgtcagta tcgctgttga tccggagtct
     1681 cctgaatcgt tcatgaggcg gcctaaacgt cgccgttggg ttaatgagaa atacacgcgc
     1741 tgggtaaaga cacagccgtg tgcgtgttgt ggtaagcctg ctgacgatcc gcatcacctg
     1801 attggtcatg gtcagggggg aatggggaca aaggcccacg atattttcac gctaccgttg
     1861 tgccgggagc accacaacga acttcatgca gacccgctga agtttgagaa aaagtacggc
     1921 tctcagattg agttaatttt tcgttttctt gatcacgcct ttgcgactgg cgtgctcggg
     1981 taaaagaggt gactgatgct catagatttg gttttacctt acccgccgac ggtgaacact
     2041 tactggcgac gccgtggcag cacatatttt gtatcaaaat ccggggagcg ttatcgccgg
     2101 gcagtggcac ttattgttcg ccagcagcag ttgaaattaa gcctgtccgg acggctggca
     2161 ataaaaatta ttgcagagcc accggataag cgtcgtcgtg acctggacaa tattctgaaa
     2221 gcaccgctgg atgcgctgac gcacgcgggg ttgttaatgg acgatgagca gtttgatgaa
     2281 atcaatattg tacgtggcca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
     2341 ataatgcttg aagggcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
     2401 caaaatcccc cggcctgact gatgcatcgg cctcaacgtc gaaagatgcg ctgagtatca
     2461 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
     2521 tccttgggaa aatgggcatt agtatgaatg acagagagcg ggcaacagaa ttgctgacag
     2581 aatatgcact cagtcggtgt gatcgcgtgg cggcgttaag aaaactcccg gcagaaataa
     2641 aaccggcagt gatgcgtatt atggcttcgt atgcgtttga agattatgcc cgtagcgcgg
     2701 cgagcaaaaa acagtgcccc tgctgtcacg gaaaaaaatt tattgaaagc gaggttttta
     2761 caaacaagat ccagtatccg gatggtaagc cgccagtgtg ggcaaagtgc acaaaaggcg
     2821 tgtatccgtc ttactgggag gaatggaaaa aagtcaggga ggtggtaaaa gttgcctgtc
     2881 cggagtgtgg agggaaggga gaggtatcca ctgcctgtaa agattgtcgt gggcgcggtg
     2941 ttgccattca tcgtgaagag tcggaaaaac agggtgtgcc ggttttcaga aactgtcagc
     3001 gttgtggtgg gcgtggctat gaaagattac cttcaacgga ggcatttaat gccatatgta
     3061 atgtaaccga tgccatatct ctcgatacat ggaaaaaaac agttaaacgt ttttacgata
     3121 cgctggtggt gcagtttgat attgaagaag catgggcaga acaacaactg aaaaaggtga
     3181 ccagatagct ttgttgattt ttcccgaatc tgtggtaaat ttgccctaac gatgggtgtt
     3241 ttatgcctga cgttagaaga ttttttacac cccgccgcct ggcgggtttt tttatgactg
     3301 aaatcacgcc agtacagtaa acgcgctggt ggcggtgaat accggtcttt cagcttgctg
     3361 gctttttcga caagagttat tggtgtgtca cgttaaccgg aaaagggaaa aagacatgct
     3421 gaaacagcag gatatgaccg aaaccgccag agtggtgttt aatgaattaa gcgtcaccga
     3481 accggcgact gtcggggaga ttgcacagaa tacttacctt tcacgtgaac gctgccagtt
     3541 aatactgacc cagctggtta tggcggggct ggcagactat cagttcggtt gttacagacg
     3601 ccttcagtcc tgaaggcttt tttatttgtg gtaaatgggc ggctggtggg tgttaggggc
     3661 actcaccagc catctgctca tgcgtccgga tcacaagcaa acctcaggcc cactgctttg
     3721 cgcaaaagca gaatgagcct atcagagaca ggcttaatga tccatgctta gtactgtaaa
     3781 aatatccagt tgtgagttaa tcaacgccga ctgcctggaa tttatccggt cgttacccga
     3841 aaattctgtt gacctgatag tcacggaccc gccgtacttt aaagtgaagc ctgagggctg
     3901 ggataaccag tggaagggcg acgatgatta cctgaagtgg ctggaccagt gtcttgcgca
     3961 gttctggcgg gtgctgaaac ctgccggaag tctttacctg ttctgtggcc atcgcctggc
     4021 atctgacatt gaaatcatga tgcgtgaacg cttcagtgtg ctgaaccata ttatctgggc
     4081 gaagccgtcc ggacgctgga acgggtgcaa caaggaaagc ctgagggcgt atttccccgc
     4141 cacagagcgc attctgttcg cggaacatta tcaggggccg tatcgtccga aagatgccgg
     4201 gtatgaggcg aagggcaggg cactgaaaca gcatgtgatg gccccgctga ttgcttactt
     4261 tcgtgatgca cgtgctgccc tggggataac ggcaaaacag attgctgatg ccacaggaaa
     4321 gaaaaacatg gtgtcgcact ggttcagtgc cagtcagtgg cagctaccgg acgaaagcga
     4381 ttatctgaaa ttacaggcgc tgtttgcccg ggtggcagaa gagaagcatc agcgcggaga
     4441 actggaaaag ccacatcacc aactggtcag cacatacagt gaactgaacc gacagtatac
     4501 ggaactgcag agtgaatata agcatctgcg gcggtatttc ggtgtgacgg cgcaggtgcc
     4561 gtacaccgat gtgtggacgc ataaaccggt gcagtactat cccgggaaac atccgtgcga
     4621 aaaaccggca gaaatgctgc agcagataat cagcgcgagc agtcgtccgg gtgacttggt
     4681 tgcagatttc ttcatggggt cgggctcaac cgtcaaagca gcgctggcac tggggcgtcg
     4741 tgcaactggt gttgagctgg agagtgaacg ttttttacag acagtgaagg agatccggga
     4801 cgatttttgc aggtgacaga ggttgaggaa aggttgaagg gaggatcatt tgttattgtt
     4861 gtatataatt attctacccc tttcctgagt caggtaaata tataatggca gttgtctgtt
     4921 ctgtaattct ggtttgttcc ccgatcaata tttttattga gaaagacaaa ctttcacttg
     4981 tgccgggcat ggtcattctg gtcaccagaa acatcaggga gctttttcgc acttacacag
     5041 accgggttaa ggttgctgat ataaatcacg atatagtcag tcaatatctg gaaggtgggc
     5101 gaaggcggat gagctcccca caggcatgtt ttccgcatta tctcacttct ggcattcatc
     5161 agcctgtact ggcagaggcg ttaattacta atcatggatt tcccagtgct ccttctggtg
     5221 atttgtccac gatgacaggg ctttcatgtc tttctctgtt tgccaacgat gagagcctgt
     5281 catcattttt gtcaaggtgt ttaagcaata caagcgataa agtcagggca ataattcaga
     5341 gcgatatatc cgtaaactgg aagcttggtg ttattgcgtt ac
//
LOCUS       DAZGHF010000225         5355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGHF010000225 DAZGHF010000000
VERSION     DAZGHF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.226"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..207
                     /locus_tag="ACHK3A_004776"
     CDS             <1..207
                     /locus_tag="ACHK3A_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8742744.1"
                     /translation="ATGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAL
                     YIDGRREMMQWYADYIDSLSEQVS"
     gene            536..1117
                     /locus_tag="ACHK3A_004777"
                     /pseudo
     CDS             536..1117
                     /locus_tag="ACHK3A_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            1204..1482
                     /locus_tag="ACHK3A_004778"
                     /pseudo
     CDS             1204..1482
                     /locus_tag="ACHK3A_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1750..2754)
                     /locus_tag="ACHK3A_004779"
     CDS             complement(1750..2754)
                     /locus_tag="ACHK3A_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO8742745.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKVLLRKKFTRTKLIEFLAG
                     CASSTVVMEACAGAHFMARRIADLGHEAKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEAQQAMRALHRVRESLVSDKVKTTNQMHAFLLEFGISMPTGVAVVKR
                     LSTVLAENELPPYLAQLLMRLHAHYIYLVGQITELEIALEQELSRDEVGQRLQTIPGI
                     GPITASVLSSQLGDGKQYSCSRDFAASTGLVPRQYSTGGKNTLLGISKRGDKNLRRLL
                     VQCARSFIQRIENQSGRLADWVRALLARKHSSVVCCALANKLARIAWAITTQQTVFER
                     "
     gene            2803..3758
                     /locus_tag="ACHK3A_004780"
                     /pseudo
     CDS             2803..3758
                     /locus_tag="ACHK3A_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3798..4277
                     /locus_tag="ACHK3A_004781"
                     /pseudo
     CDS             3798..4277
                     /locus_tag="ACHK3A_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            4420..4649
                     /locus_tag="ACHK3A_004782"
                     /pseudo
     CDS             4420..4649
                     /locus_tag="ACHK3A_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4636..>5355)
                     /locus_tag="ACHK3A_004783"
     CDS             complement(4636..>5355)
                     /locus_tag="ACHK3A_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8742746.1"
                     /translation="ALAAKAGYSTWWISNQGKLGEHDTRISVIASDAEHTVFLKKGSF
                     ASRKTDDMLLLQETERALADKSSPKVIFLHMIGSHPNPCDRLNSWPNHYLEQYPRKIA
                     CYLASISKLDNFLGQLDGILRRHSRHFAMLYFSDHGLSVSDSANPVHHDGHVQGGYSV
                     PLIITASDITSHQSVSRKISARHFAGIFQWLTGIRTENIPPFNPLTDEDNEPVMVFNG
                     ERNVPADSLKPQPLILPVRGK"
BASE COUNT         1463 a         1228 c         1278 g         1386 t
ORIGIN      
        1 gcaaccggtc atggttttcg tcacaccatg agtaccattc tgcacgaaca ggggtttaat
       61 tctgcctgga ttgaaatgca gttagctcat gtggataaaa attccatcag gggtacctat
      121 aatcatgccc tgtatattga tggtcgccgt gaaatgatgc agtggtatgc tgactacatt
      181 gattcgcttt ctgagcaggt aagttaagct gagaacacgt tattctgatt ttcactcact
      241 tcacgattat tcctccggcc tgcaggttcg gaggattctt atttttatgt tttattttca
      301 gataaagttt ttgacaaaac gtttattctg catcagcaaa attatcacaa aacaacgcaa
      361 tcacagacaa attaacattt ttcagacggg tatattgagt gcccacaatg taagacaggc
      421 aggcatccgg tgacatggta aaatattaat ccagggcaaa aacaaggctt gatacacaac
      481 atcatgactt ttatgctctc agactatgcc accacagatt ttacaggctc agaaaatgaa
      541 tgacagatta cgctttgaag tgaacgataa ccagggctgt ttcgtgttcc cggacgcctg
      601 gtttggctcg ttgctggatg agtttgacga actaattgat gcatacgaca gagatgaaat
      661 cagcgaaaca agttatatca acaaactcag acgccttgcc cgacaggagc ctgactttat
      721 tgatgtacac gcttacctgg catgcgcatt tctggagcag aatgccccgc gtaaggcact
      781 gaacgctgct cttaaaggac tggcagcagg taatcgtctc atcccggaaa gtttcagtgg
      841 tgaaatcatc tggatgcatc ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat
      901 tctcgcaaat gtgcacttac agcgtcatca ggatgcagct ctgctcactg ataaaatact
      961 ggcatacaat cctgaagaca atcagggagc acgctggcta ctgggaccgg aactgttacg
     1021 tactggcgac catgaacagg cacgccatgt actgaaagcg catgcagatg aattttcacc
     1081 ttactggtat gaactcggcc ttctgcattt tctgaaccgg ccccttgaaa cgccagacag
     1141 tggatgtatc ttaaaataag agataggctt ggatatatgt ctaacactag ttctaatttt
     1201 gagatgactg gaattttgct ggggcaggag cttcgtaaac gtaaaactcc tcaggaaaaa
     1261 atcgccatta ttcagcagac aatggaaccc ggtatgacgg tgtcccatgt ggcgcgtttg
     1321 catggtattc agcccagcct gttgtttaag tggaaaaaac aatatctgga aggaagtctc
     1381 actgctgttg ctgccggaga ggatgtcgtt cctgcctcag aactggctgc cgctataaaa
     1441 caaataaaag agttacagcg cttgctgggt aagaaatcta tggtccccac catttttgca
     1501 acactgattt tgattgatgt tggcttgcct aaatctatcc ggcgtcatta tgggcatgga
     1561 tgcccgcgcc tcgatgagtt ccgcacctga tgagcccaaa aaaccatccg gcttcagtga
     1621 gccattttct tgctgaggct tttcatcagg ccgatatgcc gttcatgtca tcaataatca
     1681 gtcttcgcaa aaccagattg ggaactactt taattcatga ttaacctgcc agccagactg
     1741 gcgccatcgt tacctttcga acacagtctg ctgtgttgtg attgcccacg ctatccgggc
     1801 taatttgttg gccagtgcgc aacatactac gctggagtgt tttcttgcca acaacgccct
     1861 gacccaatcc gccaaccttc ctgactgatt ctcgattctc tgaataaagg atcttgcgca
     1921 ctgaacgagt aaccgtcgca gatttttatc tccacgttta cttatcccta aaagtgtatt
     1981 tttaccacct gtgctgtact gccggggtac cagaccggtt gaagcagcaa agtcccgact
     2041 gcagctatat tgtttaccgt ctcccagctg cgatgacagt acgctggcgg ttatcggacc
     2101 aataccgggt attgtctgaa gacgttgtcc tacctcatca cgactcagtt cctgctccag
     2161 tgcgatttca agctcggtga tctgtccgac aagataaata taatgggcat gtaagcgcat
     2221 cagtaactgc gccagatagg gaggaagttc gttctctgca agtactgttg aaagtcgttt
     2281 taccactgcg actcctgtcg gcatactgat gccaaattcc agcaaaaaag catgcatctg
     2341 attggtcgtt ttgactttat cactgaccaa tgattccctg acccggtgaa gcgcccgcat
     2401 ggcctgctga gcttctgttc gtggttgcac aaatcgcatg gagggacgag atgccgcttc
     2461 gcatatggct tctgcatcga caaaatcgtt cttgttgctc ttaacaaaag ggcgaacgaa
     2521 ctgcggagaa atcaattttg cttcatgacc taaatcagca atccggcgag ccataaaatg
     2581 agctccggcg caggcttcca tcacaacggt agacgaggca cagcctgcca gaaactcaat
     2641 aagtttggta cgggtaaact ttttacgcag caataccttg cctgacttat cttggcagtg
     2701 aatgtgaaag gaatgcttgc caagatctat accaataagc gtaatgtttt gcatgatggt
     2761 cttctccatg aaagaaaaac actctactag ggtacagctc acagggtggt ggggaccatc
     2821 tcattaacca tggaagttga gatcctcaaa gaagcggtgg agtatggtca gtcgcgaaaa
     2881 tggatagcgc atgcgccctt gttgccaacg gacaaggaat aaccctggta agccgtattc
     2941 tgggcgtgtc gcgtgcgcaa ctggcacaac ggatgaaaac atctggtaat aagccgaata
     3001 aacgtcgtcg cagatgtgat gaggctgcgg atgcggaagt gttagcacgt atccttgata
     3061 tcatcagcga tatgccgaca tatggctatc gtcgtgtctg ggccattctc agacgacagt
     3121 cccgtaacaa aggagtgccg ttcgtgaatg caaagcgcgt ttatcggata atgagcgaaa
     3181 ataacctgtt gttacatgac aaaccttcac ggccgcagcg ggaacataaa ggccgaatat
     3241 cggtgaagga aagcgaccag cgctggtgtt ccgatggctt tgagttcggt tgtgatgatg
     3301 gcgcaaaact gcgtgtcacg ttcgcactgg actgttgcga tcgtgaagcc atagactggg
     3361 ccgcaagcac gggaggttat gacaaagcaa cggtgcagga tgtcatgtta ggagcaatag
     3421 aaaaacgctt tggcgataaa ggagcggaac ggccaatcca gtggctgact gataacggtt
     3481 cggcttatac agctcatgaa acacggcagt tcgccagaga gttgaatctg gagccctgca
     3541 tgacggcagt cagtagccct cagagcaatg gaatggcaga aaggttcgtg aaaacgatga
     3601 aagaagacta catcgcgttc atgccgaaac cgaatgtaag aacggcattg cataaccttg
     3661 cagtggcgat cgagcattac aatgaaaccc atccgcatag tgcgttaggt tatctctcac
     3721 caagggaata tcgacgtcag cgggtaacgt caacttaaga tacaacgact gtctgaatat
     3781 cgtgggtcaa ggacagcctt ctgcattttc ttaatgggga actggtaaaa gctgcaactg
     3841 catttcgtcg cggctttgcg gcaaatactt acatcgcaga aatactgtgc ggaaatctgc
     3901 atccatttcc acttgctgta tggcataact tttcaggcgg tccggatact gctgaagatt
     3961 attatgccac ataccacccg ttatgggggc aatatccgga agcattactg tttgtaaact
     4021 ggctctataa tcattcctct gtgctgcatg aacgggcaga aatcattaaa tgcgccgaaa
     4081 tgctgatgca ggaagatgat ttcgaaatat gcgaaagtat tttaagacag caggagaagt
     4141 tgcgtgaaag aattgatgag acgctttctg agaaaattgt acagaaatgc agaaatatga
     4201 atggtgaata tgtctggccc tggatattgc cgttttcagc ggcaggcatg aaacatactg
     4261 gcatacagta tcagtagata ttgcattagt gtatcctgca cacaagtaat aatttatcca
     4321 ccaataataa cactgttaat gtccccttcc cctggttgtc agccaggggt tatcttctga
     4381 atatttcttt tgaaaaggat aacacagtaa attattttta tgaattatcc catggactca
     4441 ttaacaccct ttcataatgc tttattgtca aacacgttat ggctgacatc aaaaaaaccg
     4501 gatttcctct gccagcgggt aatcacctcc ccggtgtttt cggttggtct ggttactcca
     4561 gtctggtaat tagcaagata atcgatataa acagtggaaa actcatcgta cataatctgg
     4621 tgatgaacat tacgcttatt ttcccctgac cggaagaatc agaggctgcg gtttcagact
     4681 gtctgccggt acattcctct ccccgttaaa aaccataacg ggttcattat cttcgtctgt
     4741 cagcggattg aatggcggta tattttcagt acgaatgccg gtcagccact gaaaaatacc
     4801 tgcgaaatga cgggcactga ttttcctgct gacggactga tgagacgtga tgtcactggc
     4861 ggtaataatc aggggaacgc tgtagcctcc ctgcacatga ccatcatgat gaacaggatt
     4921 ggcactatcg ctgaccgaca gcccatggtc agaaaagtaa agcatggcga aatgacggga
     4981 atgccggcga aggataccat caagctgacc gagaaagtta tccagtttac tgatgctggc
     5041 gaggtaacag gcaatctttc ggggatactg ctccaggtaa tgattcggcc aggagttaag
     5101 tcggtcacac ggattcggat gagagccaat catgtgcagg aaaatcacct tcggagagga
     5161 tttatccgcc agcgcacgtt ctgtttcctg taacaacaac atgtcatccg ttttacggga
     5221 agcaaagctg cctttcttga ggaaaacggt atgctccgca tcagaagcaa taacagagat
     5281 gcgtgtatca tgctccccca gttttccctg attggatatc caccatgtgc tgtatcctgc
     5341 ttttgctgcc agcgc
//
LOCUS       DAZGHF010000226         5350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGHF010000226 DAZGHF010000000
VERSION     DAZGHF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.227"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..148
                     /locus_tag="ACHK3A_004784"
     CDS             <1..148
                     /locus_tag="ACHK3A_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO8742747.1"
                     /translation="NLRKEFFRVKLEKIINEVERHHGQMEYVADPAALQYLQSLEYAE
                     NEAA"
     gene            383..2128
                     /locus_tag="ACHK3A_004785"
     CDS             383..2128
                     /locus_tag="ACHK3A_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO8742748.1"
                     /translation="MTPGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPTERSAQQLQDVMTLRGVPFATCFDREGKKTYS
                     RRKDVVHLLMYQSSKGLEFPYVAVINASFVHSGAADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            2194..2499
                     /locus_tag="ACHK3A_004786"
     CDS             2194..2499
                     /locus_tag="ACHK3A_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742749.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            2554..2868
                     /locus_tag="ACHK3A_004787"
                     /pseudo
     CDS             2554..2868
                     /locus_tag="ACHK3A_004787"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            3193..3342
                     /locus_tag="ACHK3A_004788"
                     /pseudo
     CDS             3193..3342
                     /locus_tag="ACHK3A_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3517..3951
                     /locus_tag="ACHK3A_004789"
                     /pseudo
     CDS             3517..3951
                     /locus_tag="ACHK3A_004789"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            4094..4183
                     /locus_tag="ACHK3A_004790"
                     /pseudo
     CDS             4094..4183
                     /locus_tag="ACHK3A_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4588..>5350
                     /locus_tag="ACHK3A_004791"
     CDS             4588..>5350
                     /locus_tag="ACHK3A_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HFO8742750.1"
                     /translation="MAKMIPAFGPIDNNSYGEKTVYTLLKEGLSDDFTVIHSLPWLSA
                     ATRNLGMKLPPSGEIDFLVLHPVYGVLALEVKSGIYRVEGTVFVHIKTKKHVDIVKQT
                     RNNVHGLARWLGGATTLRLRIGYGFIFPDSYFDDKIINAAMVDASVKPFRGIFVDKSQ
                     LPEMAKHTIDIMQYWKHALGNNELGDERVQLIIKSVCPEFDGTPDWGIRIIYDNKLWL
                     RLTHEQIKVIELLSEYQRVVVTGWPGTGKTLIGIEF"
BASE COUNT         1422 a         1175 c         1407 g         1346 t
ORIGIN      
        1 taacctgcgt aaagaatttt tccgcgtgaa gcttgagaaa atcattaatg aagtggagcg
       61 tcaccacggg cagatggaat atgtggctga cccggcggcg ctgcagtacc tgcagagtct
      121 ggaatacgca gaaaatgaag cagcctgaca gtcagtgttc tgattttacc ccggccggat
      181 ataacagaag ggatatctgc agataccctg cagcatggtt cacagtattc tggtcatgtt
      241 aatgattctg ttttttattt ctgaataaag cgacacccac gatataacat ccgtggatgt
      301 cggtggtgat tattttttgt tcacagatta aggtgcgttt atggcgaaac tgataaatgt
      361 atcggcctcc actgaggaga gaatgacgcc tggggagagg agggtggctt cacgactgga
      421 gagctttctg aatgatgact gccttgtctg gtacgatatt ccggtgggac ggaaaaaccg
      481 gcatccggat tttgtcatta ttgacccgga gaacggactg gtatttctgg aggtcaagga
      541 ctggacggtt tccacgctgc gccaggtaaa tcaggagcag gtcacgctgg aaaccgacgg
      601 tcttctgaaa tcagaaatca atccgctggt acaggttcgt cgttatgcct gtgatacggt
      661 taatgcctta cctgcaaacc cgtgcttacg gcagaatgac ggtcagtata agggacggct
      721 gaacctggcc tgggcgtatg gtgtggtgtt tacccgcatt acccgtcagc aactgaagac
      781 actggcaggg aatgatgaga atgcggtgga aaaaattttt ccttcagcac agacaatctg
      841 ccaggatgaa atgacgcagt ctgttttacc tgaggtattc cagcaaaaaa ttgccgggat
      901 gtttaccacg ggattcagaa cccgggtgac accccggatg agagatattc tgcgcgccca
      961 tctgtttcct gaagtgaccg tgaaacagaa ctcacaaatc aaaatcatgg atattcagca
     1021 ggagattctg gccagaaata ttggtgacgg gcatcgtgtc attcatggtg tggccggttc
     1081 cggaaaaacc atgattcttt tgttccgttg cctttacctg gctgaaacca cacccggaaa
     1141 gattctggtg ttgtgctaca acattaccct ggccagctat ctgcgggaat gcattgaagc
     1201 cagagggttg gcgtcacggg ttacggtatc ccattttcac agctggtgtg cctcaatggt
     1261 aaaacgccac ggtattaagg tgacggccga cagaaaagaa tatcctgata aatgcttcag
     1321 tgcactggaa gaggccgtga acagcggaaa aatcacccga accggatatg atgctgtact
     1381 ggtggatgag gggcatgatt ttgacagtcg ctggctggcc ctgattgccc gtctgtttga
     1441 taacgcatcc cgttcactgc tcctgatgta cgatgacgcc cagtccattt accggcggga
     1501 gcgggccctg aacttttctc tggccagtgt ggggattcag gcacagggac gcacttctgt
     1561 cctgccggtc aattatcgta atccgaaacg cattctgcac tttgcttatg cattctcgcg
     1621 cgagtatttt gaaaaacatc agaaccgtga aataccgctc gttcagccac aggcgggcgg
     1681 tgaagagggt acggagccgg aaatcctgcg ttgtgcttct gaacgtgatg aagccagaca
     1741 agttgcagac tggctggaga agcgccatgc cctgtgtggt cactggagtg atatggctgt
     1801 gctgtgtccg acagagcgtt cagcgcaaca attgcaggac gttatgacac tacgcggggt
     1861 gccttttgcc acctgttttg acagggaagg caagaagaca tactcgcgac gtaaggatgt
     1921 ggtgcattta ctgatgtacc agagcagcaa ggggctggag ttcccgtatg tggcggttat
     1981 caatgcgtct ttcgttcact ctggtgcagc ggatgagtct gaagtgattc cggtgcttta
     2041 tgtggcgttc acccgggcaa cccgtgaatt actggtgacc tgttaccgtg aaaacagtat
     2101 cagtcgtcac cttgaggatt ttgcataata ccggattatt tctttattcc ggactttcat
     2161 catactgtta ttatcaggtt tatttaattc gccatgaacc cgttatttaa tgatattcag
     2221 atgcgtcttt tttatctgaa ccactctccg tactcctggc actggaatgt cagattcaga
     2281 ccccaggaag ctgtctatat tggcagcgat acctgtcata tcaccattac ctgtaatcag
     2341 tccggttttc atttaaccag ggacggacaa cggctgttca ctgaacgtta tatcagaaac
     2401 cttaatgagc tgttaccggt tctgaaacgg cggtgggatg tgacaccggc aatcatccgt
     2461 gccgtggaat acctttcccg ggtcccggta ttacactgac attcttttcg tcactgttaa
     2521 tcatgcttta atacagtaac accgaatctg ccgatgactt ctgaagtgaa actgaaaacc
     2581 ggtggtgacc ccaggtcatt accggattat gctgccctgc gtgatgaaat cagcaaactg
     2641 acccacccgg cacgtccgga tgtggactgg cgttacgtgg aaacgctctg cctgcgtctg
     2701 tatgaacaca atggcgtgga attacagacg gcatcctggt acaccatcgc ccggatgcat
     2761 acagcagggt taagtggact gaatgaaggg ctggctctga ttgtggcgct gacccggcat
     2821 cactggtcag tgatgtggcc gctgaatacc cacgcccggc tggaaattat taccggtctg
     2881 ttcaaccgtc tgcaaaaaac gcttcgggcc atgcctccgg acgaccgtga caacctgccc
     2941 ctgctgtatc agaccgaaac attcctgaag gcactgagtg acacacttgc ccggcacgaa
     3001 cttaaacagg gcagtaaagt ggctttaccg gaagccatgg tgaagggata tatcacccgc
     3061 ctggaaaatc agcctgtgca gggcgaagcg tcgtctccgg taacgcttcc ggctcaggca
     3121 ctccggtctg atgctccgga tgtgcaggaa catcagaccc tgccgcactc ccgtctggtg
     3181 tatgttgttt ctgaaacaaa agcagagccc acaccctccc ttcagaaatc cccgcccatg
     3241 tttctgaagc cgtttttggc tggtgtctgt gcggcattgc tggctgtcag cgtggccatt
     3301 ccgggatggc agtttctgac acagccatca ccggcggagc agcaactcag ggcgctgctg
     3361 acacccccac cggatgtgct ttcggcagac cagatagcac agctcagcag aacacgctca
     3421 ctgttaaatc atacgtcaga gtggataccc ctttccggtc aacagataac gcgactgaca
     3481 gaattacccc tggcatacaa cctgcagcgc agtgcgcagc tgctgcagca actgagggta
     3541 cttttccccg ataatccccg ggtacaggaa atggtggata actggcagaa gagtgtaagg
     3601 agccgtgcgc tcccggaaga ggcgatgacg ggctggaacg aaggcatgac ccgcctacag
     3661 cagctggctg agcgcttgaa ccgtctggat gaacagcggg gaaaatacat gacagtcagt
     3721 gaactgaaaa cagaggtgtt tggcatcatg caggctttta accggcatat cccggcagaa
     3781 gagcagttac gtcgctataa tgaagtccgc aaccagaatg gcagtgaaca acagcaaaaa
     3841 ctgacgcagg atgccctgac ggaactgttg aaccgctact ggtttctgag acatggagat
     3901 gcaggtaaga agacgataaa taataaacag gataaccaga ttaatatctg acagatttta
     3961 ttgccattat ttatccagaa gcgatgatgc cattaatgca aaatgacatc tgaattgatc
     4021 acagattgga ccaatctgtg atcaaataga acagaatacg cagatgataa agcggctttc
     4081 cctcgaagat tcacaacgtc atctggcaga atatgggtga gagttgcagg ctgatgcgta
     4141 caaagcttac gatgcgttgt atgaaactgg ccgggtgaaa gatgtcatcc gtacggtgcg
     4201 cgccagacta aacaggcatc atgatggaaa agtcgggtat cattcacagg catatttgtc
     4261 agaatggtag cgtcagcggg ttttgattac accagttttg aagctcatac ttcctatgag
     4321 gcactgcaga aaacgaaagt gtcgtttagg cgctggggga ggggttaaaa aagtaacggc
     4381 agaagtcacc ctgaagttga ggcagtcaac tactgttagc tggctgctgc atgagagtgt
     4441 ggagggagat tgtatatatt ggttagtcag agtctgcttc ggaataagta tgagtaattt
     4501 ttaaaatggg cggaagttat ttttaattac tgggctatgt aacaaatgca gattcctgtc
     4561 agaaacatgt tataaggaaa tagtaatatg gccaaaatga ttccggcatt tggacccatt
     4621 gataataata gttatgggga aaaaacagtc tatacactgt taaaagaagg tctgtccgat
     4681 gatttcacag tgattcatag tctcccgtgg ttaagtgcgg cgacacgaaa tttagggatg
     4741 aaattacctc cgagtggaga aattgatttt cttgttctcc atcctgtata tggtgttttg
     4801 gcgttagagg taaagagtgg aatatatcgc gtagaaggta ctgtttttgt acatataaaa
     4861 acaaaaaaac atgtagatat agtgaagcaa acacgtaaca atgtccatgg tttagcccgt
     4921 tggttagggg gagcaacaac gttacggttg cgtattggtt atggttttat ttttcctgat
     4981 agttatttcg atgataaaat tataaatgca gcaatggtag atgcgtctgt taaaccattc
     5041 aggggaatat ttgttgataa aagccaactc ccggaaatgg caaagcatac tattgatata
     5101 atgcaatact ggaaacatgc attgggaaat aatgagttgg gggatgaacg ggttcaacta
     5161 ataataaaat ctgtatgtcc tgagtttgat ggtactcctg actggggaat ccgtattatt
     5221 tatgataata aattatggtt aaggctcaca catgaacaaa tcaaggtcat tgaacttctg
     5281 tcagaatatc aaagagttgt tgtaaccggg tggccaggaa caggaaagac cctgattgga
     5341 attgagtttg
//
LOCUS       DAZGHF010000227         5207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGHF010000227 DAZGHF010000000
VERSION     DAZGHF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.228"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..129
                     /locus_tag="ACHK3A_004792"
     CDS             <1..129
                     /locus_tag="ACHK3A_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8742751.1"
                     /translation="VAVMPHAAKVLKQVMVNTGGNKEQVPAKRQNRGSRNSTDDGG"
     gene            155..676
                     /locus_tag="ACHK3A_004793"
     CDS             155..676
                     /locus_tag="ACHK3A_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8742752.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            734..967
                     /locus_tag="ACHK3A_004794"
     CDS             734..967
                     /locus_tag="ACHK3A_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO8742753.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1028..2986
                     /locus_tag="ACHK3A_004795"
     CDS             1028..2986
                     /locus_tag="ACHK3A_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO8742754.1"
                     /translation="MSVTKSEPKSTRKASRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQTVAEHIREAEGWEWCAGRMEPVGFCREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQSEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMLQPE
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDATERTDNLADAA"
     gene            3041..3475
                     /gene="psiB"
                     /locus_tag="ACHK3A_004796"
     CDS             3041..3475
                     /gene="psiB"
                     /locus_tag="ACHK3A_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO8742755.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGESFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVSDIIHILMVEGGQV"
     gene            3472..4191
                     /locus_tag="ACHK3A_004797"
     CDS             3472..4191
                     /locus_tag="ACHK3A_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO8742756.1"
                     /translation="MSVRSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERRRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4188..4502
                     /locus_tag="ACHK3A_004798"
     CDS             4188..4502
                     /locus_tag="ACHK3A_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFO8742757.1"
                     /translation="MKWRYSLRWKLPSPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVCRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            4651..4800
                     /gene="mok"
                     /locus_tag="ACHK3A_004799"
                     /pseudo
     CDS             4651..4800
                     /gene="mok"
                     /locus_tag="ACHK3A_004799"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4742..4867
                     /locus_tag="ACHK3A_004800"
     CDS             4742..4867
                     /locus_tag="ACHK3A_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO8742758.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGDREVAAFLAYESGK"
BASE COUNT         1169 a         1351 c         1631 g         1056 t
ORIGIN      
        1 gtggcagtga tgccccatgc ggcgaaggtt ctcaaacagg tgatggtgaa taccggaggg
       61 aacaaagagc aggtccccgc aaaacgtcaa aaccgcggct cccgaaactc aacagatgat
      121 gggggctgaa aacatcaaca gaaggagaca catcatggca gttcgtggca ttaacaaggt
      181 cattcttgtc ggtcgtctgg gaaaagatcc ggaagtccgt tacatcccca acgggggcgc
      241 agtggcaaac ctgcaggtgg ccacgtctga aagctggcgc gacaaacaga cgggggaaat
      301 gaaggaacag acggaatggc accgcgtggt gctgttcggc aagctcgcgg aagtggcagg
      361 tgaatatctg cgtaagggcg cgcaggtcta catcgagggg caactccgta cccgtagctg
      421 ggaagataac ggtatcaccc gttacgtcac tgaaatcctt gtgaaaacca cgggcaccat
      481 gcagatgctg gggagtgcac cacagcagaa cgctcaggca caacctcagc ctcagcagaa
      541 tggtcagcca cagagtgctg acgcgacgaa aaaaggtggc gcgaaaacga aaggccgtgg
      601 acgtaaggcc gcgcagccgg agcctcagca gccgtctgaa cccgcgtatg acttcgatga
      661 cgatattccg ttctgaacgg gctgactgtg ataaccgccc cgtcctgcgc ggggcatcac
      721 cggagagatg aggatgagcg aatatttcag aatacttcag ggactgccgg acggtccttt
      781 tacccgcaaa caggccgaag ccgttgccgc acagtaccgg aacgtcttta tcgaggatga
      841 ccacggcgaa cagtttcgcc tggttgtccg taataacgga acaatggtct ggcgtacctg
      901 gaattttgag gacggtgcag ggtactggat gaaccatgtc atccgtgatt tcgggatcct
      961 taagtaagag aggtgccgga cggggatcac gtccggcaga cattaactac acaataaggg
     1021 gatagtcatg tcagtaacaa agtctgaacc aaaatctacc cgtaaagcaa gccgtaaacc
     1081 tgcaaaaacg caggaaactg tcctgtctgc cctgctggcg cagacggagg aagtgagcgt
     1141 gccgctggcc tcgctgatta aatcacctct gaatgtgcgc acggtgccgt attctgcgga
     1201 gtccgtcagc gaactggcgg agtcaattaa aggtgtcggc ctgctgcaga atctggttgt
     1261 tcatgccctg ccaggtgacc gccacggtgt cgccgcaggc ggtcgccgac tggcagcact
     1321 caacatgctg gcagagcgtg gcatcattcc ggctgactgg cctgtccgcg taaaaattat
     1381 tccgcaggaa ctggcgacgg ccgcatcgat gaccgagaac ggtcatcgtc gggatatgca
     1441 ccctgccgaa cagattgccg gattccgcgc aatggcgcag gaaggcaaaa cacctgcaca
     1501 aatcggtgat ttgctgggct attctccccg ccatgtccag cgaatgctga aactggcaga
     1561 ccttgcgcct gtcatcctcg atgcgctggc agaagaccgc atcaccaccg aacactgtca
     1621 ggcgctggcg ctggagaacg acaccgcgcg tcaggtgcag gtgtttgaag ccgcctgcca
     1681 gtcaggatgg ggcggtaaac cggaagtgca gaccattcgt cgtctggtga ccgaaagtga
     1741 agtggcggtg gcggggaaca gtaaattccg cttcgtgggg gctgatgcct tctcgccaga
     1801 tgaactgcgc actgacctgt tcagcgacga cgagggtggc tatgtggact gcgtggcgct
     1861 cgatgccgcc ctgctggaaa aactccagac tgtcgctgaa cacattcggg aagccgaagg
     1921 ctgggaatgg tgcgccggac gtatggagcc tgtcggtttc tgccgtgagg atgccggaac
     1981 ataccgttgt ctgccggagc cggaagcggt gctgacggag gcagaagaag aacgcctgaa
     2041 cgaactgatg acgcgttacg acgcgctgga aaaccagagt gaggaatccg acctgctgga
     2101 agcagaaatg aagctgatgc gttgcatggc gaaggtcagg gcgtggacgc cggagatgcg
     2161 tgccggaagc ggtgtggtgg tgtcctggcg ttacggtaat gtgtgtgtcc agcgtggtgt
     2221 gcagttgcgc agtgaggatg acgtggctgc cgacgctgac cgcacggaac aggtgcagga
     2281 gaaagcgtca gtggaggaaa tcagtctgcc actgctgacg aaaatgtctt ccgaacgcac
     2341 gctggcagtc caggcggcac tgatgctgca accggaaaaa tctctggcac tgctggcatg
     2401 gacgctctgc ctgaatgtgt ttggcagcgg agcgtacagt aaaccagcac aaatcagcct
     2461 ggaatgtaaa cattattcgc tgaccagcga tgcgccatcg gggaaggaag gtgccgcatt
     2521 catggcgatg atggcagaaa aagcccgtct tgcagccctg ctgccggagg gatggtcacg
     2581 ggacatgacg acgttcctgt cactcagtca ggaggtgctg ttatccctgc tcagtttctg
     2641 caccgcgtgc agcatccacg gcgttcagac ccgcgagtat ggtcacacgt cacgcagtcc
     2701 gctggactcg ctggagagcg ccatcggctt tcacatgcgt gactggtggc agccgacaaa
     2761 agcaaacttc ttcggacacc tgaaaaagcc gcagattatc gatgccctga atgatgccgg
     2821 actgtccggt gctgcgcggg atgcggagaa gatgaagaaa ggtgatgcgg ctgaacatgc
     2881 agagcaccat atgaaagaca accgctgggt tccaggctgg atgtgtgcac cacatccaca
     2941 gacagatgcc actgaacgca ccgataacct ggctgatgcc gcctgatgaa caaccacacc
     3001 gccccgccgg agacggggcg gcagcaaggg agatactgtg atgaaaactg aactgaccct
     3061 gaatgtatta cagaccatga gtgcacagga atatgaagat atccgggctg ccggaagtga
     3121 tgaacgtcgt gaactcacac atgccgtgat gcgggagctg gatgcgccgg ataactggac
     3181 gatgaatggc gagtatggca gcgagtttgg cggctttttc cccgtccagg tccgtttcac
     3241 acctgcccac gaacgttttc acctggcgtt atgttcgccg ggtgatgtct cccaggtctg
     3301 ggtgctggtt ctggtgaatg ccggtggtga gtcgtttgcg gtggttcagg tgcagcggcg
     3361 gtttgcaccg gaagccgtca gccattcact ggcactggcg gcgtcactgg atacgcaggg
     3421 gtacagcgtc agtgacatca tccatatcct gatggtggaa ggaggtcagg tatgagcgta
     3481 cgttcacagg cactggttcc cctcagcaca gagcaacagg ccgcatggcg ggcggtggcg
     3541 gagacagaaa aacgccgcca tcagggtaac acactggcgg aatatccgta tgcgggggcg
     3601 tttttccgct gtctgaacgg aagccgccgg atttcgctgt ctgacctgcg atttttcatg
     3661 ccgtccctga ccgcagaaga actgcacggc aaccgtcttc agtggctgta tgccatcgat
     3721 gtgctgattg aaacacaggg cgaggtctgc ctcctccccc ttcccggcga tgcagcagag
     3781 cggttgttcc cgtcggtgcg gttccgtgtc agggaacgcc gtcgccataa atctgcgctg
     3841 gttatgcaga agtacagccg ccagcaggca cgggaggcag agcagaaagc ccgtgcgtac
     3901 caggcgctgg tcgctcaggc tgaaatcgaa ctggcgttcc attcgccgga aacagtcgga
     3961 agctggcatg cacgctggag tgaccgggtg gcagagcatg acctggaaac cctgttctgg
     4021 cagtggggtg agcgttttcc gtcactggcc ggaatggaac gctggcagtg gcaggatatg
     4081 ccgttctggc aggtcatcgc agaggcaggt atggcagcaa gagaggcggg tcacgctgtc
     4141 cgtgagatgg agcggtggat ggtgccgaac aaactccggg aggcggcgtg aagtggcgtt
     4201 actcactgcg ctggaaactg ccttctccct gtcctggcga gcatgaactg gtttcagaag
     4261 tggtggaggc ggggcagcct gcaccggcat ccgttatgtc acgctgggtt gccggagcag
     4321 ggtatgcggt ctgtctggat tttatttctg accgcccggt gtgccgctgg agcgaagaac
     4381 gtaaggctgc ggtacggcgg cggaatctgg agaaacgtat caacaggcat gcaccactgt
     4441 ttgcggatga actgattgcc cgtgaactgg ctgaacgacc ggattatttt cagggaaagt
     4501 gagtgtggtc agcgcgcagg gatatgggct atgatgtgcc cggcgcttga ggctttctgc
     4561 ctcatgacgt gaaggtggtt tgttaccgtg ttgtgtggca gaaggacaaa agccccgtag
     4621 ttaattttca ttaacccacg aggcccctgc atgcctagac aacatcagga tagcctctta
     4681 ccgcgctttg cgcaaggaga agaaggccat gaaactacca cgaagttccc ttatctggtg
     4741 tgtgttgata gtgtgtctca cactgttgat attcacttat ctgacacgaa aatcgctgtg
     4801 cgagattcgt tacagagacg gagacaggga ggtggcggct ttcctggctt acgaatccgg
     4861 taagtagcaa cctggaggcg ggcgcaggcc cgccttttca gggctgatgc tggtctgact
     4921 gcactgaagc gcctttataa aggggctgct ggttcgccgg tggccccttt ctccttgctg
     4981 acattgtagc agcatgaaca accagactgc caggaaggcc acttccctcc gggaacggga
     5041 atgaacgtgc gcctgcggcg ctcgttcttt tcccccgcct tctctgttta tggccagttg
     5101 tcagaaatga cattaccttt cccgtgatta ttctcttttt cctcctgttc tgactctgat
     5161 tactgctgcc ctgattattg tctgttttgt tgttctcctg cttatgc
//
LOCUS       DAZGHF010000228         5118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGHF010000228 DAZGHF010000000
VERSION     DAZGHF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.229"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            143..1051
                     /locus_tag="ACHK3A_004801"
     CDS             143..1051
                     /locus_tag="ACHK3A_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFO8742759.1"
                     /translation="MKAPVSITSFRPQKSIAIVIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1097..3088)
                     /gene="tkt"
                     /locus_tag="ACHK3A_004802"
     CDS             complement(1097..3088)
                     /gene="tkt"
                     /locus_tag="ACHK3A_004802"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO8742760.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3366..4124
                     /gene="loiP"
                     /locus_tag="ACHK3A_004803"
                     /gene_synonym="yggG"
     CDS             3366..4124
                     /gene="loiP"
                     /locus_tag="ACHK3A_004803"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO8742761.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4172..5092)
                     /gene="speB"
                     /locus_tag="ACHK3A_004804"
     CDS             complement(4172..5092)
                     /gene="speB"
                     /locus_tag="ACHK3A_004804"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO8742762.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT         1224 a         1368 c         1356 g         1170 t
ORIGIN      
        1 atttattgaa tgtccgaaag catacttttc taatgcaatg ttttttcgct ataccccttt
       61 atagattctg aattctcacc tgtcggctat tttctcctca cctcatgtga catattcccc
      121 actttactca aggaagattc atatgaaagc acctgtttca ataacctcat ttcgcccaca
      181 aaaatctatt gctatcgtca ttggcgtgct tgccgtggtt gtgctgccat ttctctcgta
      241 ttacaccgtt aatgaaggtg aacgcggcat tttgctgcgc tacggaaaaa tcgtaaaagt
      301 tgccgagccg ggtctgggtt ttaagatccc ttttatggaa agcgtggaga aaatttccac
      361 gcgtaatcag gctgtggttt atcagggatt acaggcctac agccgcgacc agcaaccggc
      421 acaaatgaca gtatcagtca gcttccacat taaaccttca gaagcaggcg cggtttatac
      481 cacctacaac accattgaag cattaaaaga tcgtctgatt gtccgtcagt tgccaactca
      541 actggagaac gttttcggcc aatataccgc catcagcgcc gtacaggatc gtacaaaact
      601 ggttcaggat ctacaaaatg caatgcgtaa agccgttgtt ggcccggtgg tgattgatgg
      661 tgtccagatt gaaaatattg atttttctga cgcttatgaa aaatcgattg aagatcgaat
      721 gaaagcagaa gtcgccattg ccacacgtaa gcaaaatctg gaaacagaga aaatccaggc
      781 gcaaatagcg gtaactcagg cccaggcaga agcagacagt aaactggcgg cagccaaagc
      841 agaagctgag actattcgcg taagaggcgc ggcagaggct gaaactattc gtttaaaaag
      901 tgcggctgaa gcagaagcga tccgcctgcg cggtgaagct ctgcgggata atcccggact
      961 tgttgccctc accactgctg agcgttggga cggtaagcta cccgatacca tgatccccgg
     1021 cagtacagtt ccgtttatct ctactaagta aaaaaagggc cgctttcgcg gcccttctca
     1081 caaagcatct taccgattac agcagcgctt tcgctttcgc aacaacgttg tcaacggtga
     1141 aaccgaactc ttcaaacagt tgctctgcag gagcagattc accgaaggtg gtcatgccga
     1201 cgatagcacc gttcaggcca acatacttgt accagtagtc agcaataccc gcttccaccg
     1261 caacacgagc ggtaaccgct ttcggcagta cggattcacg gtaagccgca tcctgcttgt
     1321 cgaacgcgtc ggtagacggc atggaaacca cgcgcgcctt cacgccttcg gcagtcagtt
     1381 tttcgtaggc ggcaacagcc agttcaactt ccgaaccggt cgcgatgaag atcagttccg
     1441 gctgaccggc acagtctttc agcacataac caccgcgcgc gatgtttgcc agctgctctt
     1501 cagtacgctc ctgctgcgcc aggttctgac gggagaggat cagcgcggtc gggccgtcct
     1561 gacgctcaac gccgtatttc cacgcgaccg cggattcaac ctggtcacat ggacgccatg
     1621 tggacatgtt cggggtcacg cgcagagaag cgacctgctc taccggctgg tgagtcgggc
     1681 catcttcgcc cagaccgatg gagtcgtggg tgtaaaccat cacctgacgc tgtttcatca
     1741 gcgcagccat acgtacggcg ttacgtgcgt attccacgaa catcaggaag gtggaggtgt
     1801 acggcaggaa accaccgtgc aaggagatac cgttagcaat cgcggtcata ccgaactcgc
     1861 gaacaccgta gtggatgtag ttacccgcag catcttcgtt gattgcttta gaaccagacc
     1921 acagggtcag gttagacggt gccaggtcag cagagccgcc gaggaattcc ggcaacagcg
     1981 ggccgaaagc ttcgatagca ttctgagacg ctttacggct ggcgattttc gccggattag
     2041 cctgcagttt agcgatgaat tctttcgctt tggcatcgaa gtcagacggc atttcgcctt
     2101 tcatacggcg ggtaaattca gcggcttcct gcggataagc tttggcgtaa gccgcgaatt
     2161 tctcgttcca tgcggattct ttcgcctggc ctgcttcttt cgcatcccac tgagcataga
     2221 tttcagacgg gatttcgaac ggtgcatatt tccagcccag ctgttcgcgg gtcagggcaa
     2281 tttcagcgtc gcccagcggc gcaccgtggg agtcgtgcgt accggctttg ttcggggaac
     2341 cgaaaccgat gatggttttg cacatcagca gggacggttt gtcagtcact gcgcgcgctt
     2401 cttctactgc gcgtttgatg gatgccgcgt catgaccgtc gatgtcgcga ataacgtgcc
     2461 agccgtaagc ttcgaaacgc attgcggtgt cgtcggtaaa ccagccttca acgtgaccat
     2521 cgatagaaat accgttgtca tcgtagaacg caatcagttt acccagcttc agcgtacccg
     2581 ccagagagca aacttcgtgg gagatgcctt ccatcatgca gccgtcgccc atgaaggcgt
     2641 aggtgtagtg gtcgacgatg tcgtggcccg gacggttaaa ctgcgccgcc agcgtttttt
     2701 ctgcaatcgc cataccgact gcgttggcaa taccctgacc cagcggacct gtagtggttt
     2761 ccacgccagc ggtgtaaccc acttccggat ggcccggagt tttagagtgc agctgacgga
     2821 agtttttcag ttcttccatc ggcagatcgt aaccggtgag gtgcagcagg ctgtagatca
     2881 gcatggagcc gtggccgttg gacagcacga agcggtcacg gtcagcccag gacggattct
     2941 gcgggttgtg tttcaggaaa tcacgccaca ggacttcggc aatgtcagcc atacccatcg
     3001 gggcgcccgg gtgaccggat ttggctttct gtactgcgtc catgctcagc gcacgaatag
     3061 cattggcaag ctctttacgt gaggacattt tgactccaga tcggatgatg aagggcacgc
     3121 ccttaacgac ttgacgacag cgcgttttgg gctacgccgg aaaatttgcc aacaatttac
     3181 cgcaagccgc gcgtcatgta catggaacat ccttttgccg cttcagaaat ctctggatca
     3241 tgctcgcatg ttgcgcaatc tgctcgcccg tccgctgcgc ttttccttat actgagactg
     3301 agcgtcgatt cacctgcaaa cggcgcattt ttagaataat cctgaccttg tgcggaagag
     3361 aaaacatgaa aattcgcgcc ttattggtag caatgagcgt ggcaacggta ctgaccggtt
     3421 gccagaatat ggactccaac ggactgctct catcaggagc ggaagctttt caggcttaca
     3481 gtttgagtga tgcgcaggtg aaaaccctga gcgatcaggc atgtcaggag atggacagca
     3541 aggcgacgat tgcgccagcc aatagcgaat acgctaaacg tctgacaact attgccaatg
     3601 cgctaggcaa caatatcaac ggtcagccgg tgaattacaa agtgtatatg gcgaaggatg
     3661 tgaacgcctt tgcaatggct aacggctgta tccgcgtcta tagcgggctg atggatatga
     3721 tgacggataa cgaagtcgaa gcggtgatcg gtcacgaaat ggggcacgtg gcgttaggcc
     3781 atgtgaagaa agggatgcag gtggcacttg gtacaaatgc cgtgcgagta gctgcggcct
     3841 ctgcgggcgg gattgtcggc agtttatctc aatcacaact cggtgatctg ggcgagaaat
     3901 tagttaattc gcaattctcc cagcgccagg aagcagaagc cgatgattat tcttacgatc
     3961 ttctgcgcca acgcggcatc agcccggcag gtcttgccac cagttttgaa aaactggcga
     4021 agctggaaga aggccgtcaa agctcaatgt ttgacgacca tccagcatcc gccgaacgcg
     4081 cccagcatat tcgcgatcgt atgagcgcgg atggagttaa gtaagcattt tattacgccg
     4141 gatttgacgc ataacgcatc ttatccggcg cttactcgcc ctttttcgct gcctgaatat
     4201 acagcatctc cagcgccagc gtcgccgcag ccagagcggt gatttccgac tgatcgtatg
     4261 ccggagccac ttccactacg tccatcccaa caatgttgag atctttcagg ccgcgtacca
     4321 gtttaatagc gcgatcggag gtcaggccgc caattactgg cgtaccggtt cccggcgcaa
     4381 atgcaggatc caggcagtcg atatcaaagg tcaggtaaac cggcatatca cccacaatct
     4441 gtttcacttg ggcgataacg tcatccacgc tgcgatcgtt cacctggcag gcgtccagca
     4501 cggtaaagcc gttgtcttta tcgaactcag tacgaatacc aatctgcacg gaatgattcg
     4561 ggtcgatcag accttctttc ggcgcggtat agaacatggt gccgtggtca aattcacaac
     4621 cgttcgcata ggtgtcggtg tgggcgtcaa agtgtaccag cgccattttg ccgaaatgct
     4681 tcgcatgagc acgcagcagc ggcagcgtaa caaagtggtc accaccgaag gagagcatac
     4741 gcttaccggc agccagcagc ttctcggcgt gcgcctgcag cttctcgctc atctcacggg
     4801 catctccgaa ggcatatacc agatcgccgc agtccacgac gttcaggcgc tcacgcatgt
     4861 cgaaattcca cgggaagcga ttgtgttccc aggccagatt cgtcgaaacc tgacggatcg
     4921 ctgccggacc gtggcgacca cccgcacgac cagaagtggc catatcgaac ggcacgccag
     4981 taatcaccca gtcagcatcg ctgtcatatg gctggaagtt catcggcagg cgtaaaaaac
     5041 caaaggcgtt ggaaaccagt gagttatcgt attgatgacc taaggtgctc atggatggac
     5101 tcctattaca aagtcgat
//
LOCUS       DAZGHF010000229         5072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGHF010000229 DAZGHF010000000
VERSION     DAZGHF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.230"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            532..627
                     /gene="dqlB"
                     /locus_tag="ACHK3A_004805"
     CDS             532..627
                     /gene="dqlB"
                     /locus_tag="ACHK3A_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO8742763.1"
                     /translation="MINLLIIVLRAVVAVANALIAILELIRQFID"
     gene            complement(994..1293)
                     /locus_tag="ACHK3A_004806"
                     /pseudo
     CDS             complement(994..1293)
                     /locus_tag="ACHK3A_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1599..2264
                     /locus_tag="ACHK3A_004807"
     CDS             1599..2264
                     /locus_tag="ACHK3A_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFO8742764.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHD
                     LLSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVS
                     DIVVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDL
                     DKLLRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIG
                     ILQ"
     gene            2360..2644
                     /locus_tag="ACHK3A_004808"
     CDS             2360..2644
                     /locus_tag="ACHK3A_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO8742765.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFR
                     LPAYFEDILEAEAQRTGQSKTTVLKAALAAFDNLDENLKNHWLLESAKLG"
     gene            2674..2901
                     /locus_tag="ACHK3A_004809"
     CDS             2674..2901
                     /locus_tag="ACHK3A_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742766.1"
                     /translation="MRKRDDYYDGLIAGGTVAIAVATLLTLLITVFVNNKENSEFHEW
                     INSHCEKENVENTSFKCDDGDGNIYIVKKEA"
     gene            3567..4022
                     /locus_tag="ACHK3A_004810"
     CDS             3567..4022
                     /locus_tag="ACHK3A_004810"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO8742767.1"
                     /translation="MTTRKSGFNWYVLEFCSARTARVFSHLERLGVTFWCPLTCSHYR
                     RSDKNCFRRKEVPLFPGYVFIKIDFNKTHSTTVTSVPWVKSFVSFGGEPVPVCEEIIL
                     GLRRRETNYWGEYSPIRGIPHELAEILLENNPMKRSFLFFKYLSNTQNK"
     gene            4098..4277
                     /locus_tag="ACHK3A_004811"
     CDS             4098..4277
                     /locus_tag="ACHK3A_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742768.1"
                     /translation="MIYTKKECVWLGFLLIGVAFLGFMADDFTDNIYTFIWIMNSFFG
                     VFMLFMGVFISDDES"
     gene            4347..4592
                     /locus_tag="ACHK3A_004812"
     CDS             4347..4592
                     /locus_tag="ACHK3A_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742769.1"
                     /translation="MSTSNENLSNKKSPIYQIKQIYIKEDTKAQLMEFIKGINDECQL
                     LVKPTYIVHCLLNCALQSEGIRKEVINYVANAREKMR"
     gene            4592..>5072
                     /locus_tag="ACHK3A_004813"
     CDS             4592..>5072
                     /locus_tag="ACHK3A_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO8742770.1"
                     /translation="MSILTTGAFLALAAQCAPNVHPDTSLDVVRVESGLHQYAIAEII
                     PKNERVAEGKSFISHYPESKDEAVKIIRRIEERKRNYSVGLMQINSSNFKKLRVTATE
                     LLEPCSNLKAYQYIITDCYLRGGSLTNALSCYYSGDFITGHKVESELNNTSYIRRIGL
                     "
BASE COUNT         1542 a          942 c         1112 g         1476 t
ORIGIN      
        1 tggatgaggt tctacgctgg aactggagtc tgaagtctaa tggaacgaaa acgtacgtta
       61 gtgaagtaac tgtctgatat atcaaaacat aatggagacc ctcaaacccc aactacagcc
      121 gaacgacagg cacaaaaaaa cccgactggt aatcgggctt ttttgcattt tccagtcttg
      181 agttggtggc tctgaccgga gaaggttact ctacacctaa cgtttacatt ttattcacta
      241 tggtgctgta aagcaactaa atgtggttaa ggaaccacat ttagttgtgt aacgggtgtt
      301 ttgtgtagcg tataatcagt cagttagtca tgtagttggc ttgcaatcct gctcttcccg
      361 tatctgcggg tatgaacgat tttaaagtcc tggctggata aacaggtggt gctgtattcg
      421 gtgctctggc aggaggaggt cactctacac ctaacaatgc gagagagccg ttgtgacagg
      481 caatgtatga gtaagcaacg gtgatttcct ccagttttac tggagaaacg gatgattaat
      541 ctgttaatca tcgttctcag ggctgtggtc gccgttgcaa acgcgctgat tgcaattctg
      601 gaactgattc gccagttcat cgactgatga cagcggaaac gtaactaagg gcaggaagtg
      661 caccgcttcc tgccctttaa caaaggagga tttatggatt ttatgagcat tttagggcgc
      721 tgtttacttc ttcctcttcc ggttgccata ttcattgggt gcttactttc tcaggacgat
      781 gaccgatttt ttggcatgtt tatgagtgta gtaagtgctg ggttgtcgtg gtatatcact
      841 aagccaaaac cacaaaaggc tgaggtcgcg taagcggcct tttatttcgg tatttattag
      901 ttacaacatt cacttttctt ttttaaagca acaccttagc atagccccac ccatcataaa
      961 taatgtcagc cagtttattg agagcgccct gccctactca ctggagaatt gccgggattt
     1021 acgttttatt gcgctactga ttgtgccacg gctgcaccct agcaccttct gtacctgtgt
     1081 ccaggagcta ccgctttcta tcaacctgat aattgcatca tgtctggcct gattaggttt
     1141 acgctcttta tatttgccgt ctttccttgc tttctctatc ccctgtttct ggcgttcccg
     1201 ccgttgttca taatcccttc tggcaacagc agccagcata tccagcagca tatcgttaat
     1261 ggctgaaaac aatcggttgt caaactcact cacactcaaa aagaaccaga aaacgtaccc
     1321 ataaccagaa agtgacaatg tcatacatgt gctttacagc ataaagataa tgtaaagata
     1381 aatgcagctt taccacgtaa cgcaaaaata aagacgcaaa tacctttaca aagtaaaggt
     1441 cttataaaga caccattatc tttatttgtg ctttactcgg taaagcttat gtaatataaa
     1501 gacacggtaa agacaaaacc atctttacaa aaatcacaca ttgtaaagac aatagagcct
     1561 ttacacattc tttacatcat aaagagagga attctgaaat gggtaaaatc ttgcttgtcg
     1621 tatcagataa gggtggtgta ggcaaaagca cctacgtggc taatacaggc tcaatgctgg
     1681 tcaacaaagg taagtcggta atcatcctga agacagataa aaaccacgat ctgttgagct
     1741 ggaacgaaaa gcggacagat aacggcttac tcactatccc ggttcacgca gcttatggga
     1801 acgtcagcaa cgaaattaag cgccttagta agctgtgtga agtgctcatt gtcgattgcc
     1861 ccggccacga cagccaggag ttccgtagcg ccctgacagt atcagatatc gtggtaacac
     1921 tagttaagcc gtcctctgat ttcgaaagcg aaacactgac cagtgttaca gagaaaatcc
     1981 gcaccgctca gaaagctaac gcagccctgg aaccgtgggt gctgttcaca agaatcaact
     2041 cagctaaacc ccgccacaga aaagcggcca ttgatctgga taaattactg cgctcggaca
     2101 acatctggat tcaacctctg aaaacccgga tatccgagct ggatgtgtat gaaagcgcct
     2161 gtaatgaagg ggcaggggta catgatgtta gccgggcatc aagcctttca acagcgaaag
     2221 cacagattga actcgtggct caggaaattg ggattttgca gtaatatcaa tgtgttaaat
     2281 tattatcttt acattgtaaa gataatgtaa aggcggtatg atatttacta tcaatacata
     2341 ccgtactagg ggaaatttca tggctttaaa actaaaccgt cctaacatcg atgaatctca
     2401 gcaaccagca acaaacgcag aaaccgcgcg ttttatctcc ggcgctacca gagcgcctgt
     2461 agtgggtaaa ccaaagttga tgaatttcag actgccagcg tactttgagg atatcctgga
     2521 agcggaggcg cagagaacag gccagagcaa gacaaccgtt ctaaaggcag cgctggccgc
     2581 attcgataac ctggacgaga acctgaaaaa tcattggcta cttgagtctg caaagttagg
     2641 ttaacaagta attcgcttat ttaggtttaa aaaatgagaa agagagatga ttattatgac
     2701 gggttaattg ctggaggtac tgtggcaatt gctgttgcaa cccttctgac actgttaatt
     2761 actgtatttg tcaacaataa ggaaaattcc gagtttcatg aatggataaa ctcacactgt
     2821 gaaaaagaaa atgttgaaaa cacttcattt aagtgtgatg acggggatgg gaatatatat
     2881 atcgtaaaaa aggaagcgta attaagggcg gggagggacc attcctgtta acgccctaca
     2941 gtcgtgacga atcagccaga agtgctggat atgtgtctgc tgtttgccag acgatttttt
     3001 caagtaatac gcctgcaatc ccgattagcg caactacaac gcccgttctg ataactgttt
     3061 tcgctttcat tttaacttaa ctgatcaaga cgctgttaac tccatggtcg tacctgctgc
     3121 tggggacagt acatgtcagt acttaccaaa caggcttcaa aaaagcaaca gattcgctat
     3181 atcggaagtt tcgacggttt cggctctcct ccctgtatga cgactgcgtg tgcagtgctg
     3241 gcttggttgt tcaaaagttc aacgaaaaca gtatgaaaaa agaacatcaa aaaaaaatat
     3301 ttatctggaa attggtacta acacgatctg ttattttgtt tttttatcaa tcggtagatc
     3361 tacgatctat ttgtgtggtt gtttttagtg taaaattctg tagaaatggc ttaatataga
     3421 tctaagatct aaatattcgc atatttacag ataaaagctt ttatgtaata tgttattacg
     3481 ctattaaaag ttgttttgat tctgcgcaaa aaacagggct acggatactt catgactgat
     3541 cggggatccc cgaggcggag gtgtgcatga cgactaggaa aagcggcttc aactggtatg
     3601 ttctagagtt ttgttctgca cgtacagcac gagtgttttc tcatctggaa aggttagggg
     3661 taacattctg gtgtcccctg acttgttccc attatcgtag atcagataaa aattgtttca
     3721 gacggaaaga agtgccgctg tttcctggat atgtgttcat aaagatcgat ttcaataaga
     3781 cccatagcac aactgttacg tcagttcctt gggtaaagtc atttgtgtca tttggtggtg
     3841 agcctgttcc tgtttgcgag gaaatcattc tgggattgag aagaagagag actaattatt
     3901 ggggggaata ttctcctata cgcggaatac ctcatgaatt ggctgaaatc ttgttagaaa
     3961 ataatccgat gaaaagaagt tttttatttt ttaaatattt aagtaatacg cagaataagt
     4021 agttgaaaat cttggtttat tatcttttgt gtattcacgg agaactataa taaaataata
     4081 aattaaaaga ggataaaatg atatatacaa agaaagaatg tgtttggctt ggttttctat
     4141 taattggtgt ggcgtttttg ggttttatgg ctgatgattt tacagataat atctatacat
     4201 ttatttggat tatgaactct ttttttggtg tctttatgtt atttatgggg gtatttatat
     4261 ctgatgatga gtcatgaatc aataaatcat aggttatttg aaatgttatg ttttaatatg
     4321 caccaagtga tttttcagga gagtatatga gtacatctaa cgaaaactta tcaaataaaa
     4381 aatcgccgat atatcagata aaacagatat atattaaaga agatacaaaa gcgcagttaa
     4441 tggaatttat taaaggaata aatgatgagt gtcaattact tgtaaagcca acatatattg
     4501 ttcattgtct tttaaactgc gcattacaga gcgaaggaat caggaaagaa gttattaatt
     4561 atgttgctaa tgcacgagaa aaaatgcgct gatgagtata ctgactaccg gggcatttct
     4621 cgctctggct gcacagtgtg ccccaaacgt tcatccagat acatctcttg atgtggttcg
     4681 tgttgagtcg gggttacatc aatatgctat agcggaaatt atacctaaaa atgaaagggt
     4741 tgcagagggt aaaagcttta taagtcatta cccggagagt aaagatgaag cagtgaaaat
     4801 aatcaggagg attgaggaac ggaaacgaaa ttattctgtt ggattaatgc agattaacag
     4861 ttcaaatttc aaaaaactta gagtgacagc tactgaattg ttggagccgt gctctaatct
     4921 gaaagcgtat caatacatta taaccgattg ttatttgcga ggaggtagcc ttactaacgc
     4981 gttaagttgt tattattctg gagattttat tacaggccac aaggttgaat cagaattaaa
     5041 taatacaagt tatattcgaa ggataggatt at
//
LOCUS       DAZGHF010000230         5016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGHF010000230 DAZGHF010000000
VERSION     DAZGHF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.231"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            128..973
                     /gene="mscS"
                     /locus_tag="ACHK3A_004814"
     CDS             128..973
                     /gene="mscS"
                     /locus_tag="ACHK3A_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO8742771.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIGGIAARMISGVLNNLMLGRNI
                     DATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLA
                     AGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIVN
                     FSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMNF
                     VVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSRS
                     VSSRQ"
     gene            1099..2319
                     /locus_tag="ACHK3A_004815"
     CDS             1099..2319
                     /locus_tag="ACHK3A_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFO8742772.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
     gene            2549..4294
                     /locus_tag="ACHK3A_004816"
     CDS             2549..4294
                     /locus_tag="ACHK3A_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO8742773.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
     gene            complement(4476..>5016)
                     /locus_tag="ACHK3A_004817"
     CDS             complement(4476..>5016)
                     /locus_tag="ACHK3A_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO8742774.1"
                     /translation="KIELHRSSPFYAEPVDCVIWIKNNLIEANDYNPNVMAPSEKRLL
                     THSLLVDGFTQPVVTLAQKGKYTVIDGFHRQMLGKSTTKLEKKLKGYLPVTCIKHDNN
                     FKAYSIASTIRHNRARGKHQIDALSDIVRDLSRMGWDDLRIGSELGMDTDEVLRLKQI
                     SGLTELFEEENFSPAWTIK"
BASE COUNT         1256 a          971 c         1306 g         1483 t
ORIGIN      
        1 ggttgtcata ccggtgtata tgatgcggga taaaggcccg gagattcctg gaaataaaat
       61 gttccgggga atataaaaca gggaaaaacc ggatgtgatg gacgcttctg gcgaggcggt
      121 ttcatgaatt gtccgtaatc aggatatgtt gcccggttac gccgcgaatc ttgtggcagc
      181 agtggttatt attatcgggg gtatcgctgc acggatgatt tctggtgtgc tgaataatct
      241 gatgctgggc aggaatattg atgccacagt ggcgctgttc ctttccgcgc tgatacgtca
      301 tgccattata gcctttacgc tgattgctgc cctgggacgt gtgggggttc agacagcttc
      361 cgtaattgca gtcctggggg ctgcaggtct tgctgttgga cttgccctgc aggggtcatt
      421 gtcaaacctt gcagctggta ttcttctcgt gatgttccgt cctttcaggg ctggtgaata
      481 tgctgacctg ggtggtgttg ccgggactgt gcagaatgta catatattct caaccacgat
      541 gcgtacactg gatggtaaaa ttgttgtcat tccgaacggg aagattattg ccggagagat
      601 tgttaatttc tccagggagc ctgaacgacg aaatgagttt atcatcagtg tttcatatga
      661 tgcagatatt gactgtgtga aacaagtcct gacagatatt gtgatgtcag aagagagggt
      721 tctgaagaac agggaaatca ctgttcgcct gaatgagcct ggggagtcat ccatgaattt
      781 tgtagtacgg gtatggagca ggcgtgatga ccttcagtct gtatactggg atgtactgga
      841 gagaattaag cgggagtttg attcagaagg gataagtttt ccatatccac aaatggatat
      901 ccatcttgtt cgctccggga agcaggcaga gtcggtaacc cataacagtc gctcagtcag
      961 ttccagacag taaaatgaaa agcgcatttt atttgtgaaa gatgaacagc ctgaatctgt
     1021 caggctgttt tcattgtgtt tttatggtca tttttggtat atgtactgta tataatgtca
     1081 tctgtacaag gatataaaat gccaaagaaa ataatgacag ggattgatgt ttttgacgct
     1141 gccatggagc gtattgaatg ggtgtttgat acgttttcat cagtttgtct ttctttttcc
     1201 ggcggaaaag actccactgt cctgtttcac ctgatggcga atattgcccg ccgaaaaaaa
     1261 cgccgttttt ctgtactgtt tgttgactgg gaggctcagt atcagtgtac cattgagcat
     1321 gtcaccaggt tgagggaaat gtacgatgat atcacagata cttttttctg ggtggcgctg
     1381 cctctgacga ccgttaatgg agtgtctcag gtacagcctg agtgggtatg ttgggaggac
     1441 ggggctgaat gggttcgcca gccaccagaa gatgccataa ccagagcgtc tttttttcct
     1501 ttttatcaga atgcgatgac atttgaagag tttattccgg catttaatca ttggtttgcc
     1561 cgggaaaaag ggatggttgc tgcgattctt actggtgttc gcgctgatga gtcactgaat
     1621 cgttttatgg gactggtatc cggacgtaag ctccgttatg cggaggataa accctggacc
     1681 acggcatcgg ctgagggatt ttactacacc atgtatcctt tgtacgactg gaaaacaaga
     1741 gatgtatgga tttaccatgc gagaacacgt gctgcctata actctcttta tgacctgatg
     1801 tacagggctg gagttcctct tcgcaatatg cgggtgtgtg agccttttgg accggagcaa
     1861 cgtaaggggt tgtggcttta tcatgtactg gagccggata catggggcag aatgtgtgct
     1921 cgcgtatcag gtgctgtagg gggagcgctc tatgcaaatg aaagtggtgc ttattttgct
     1981 ttgaggaaac agataacaaa accagttcac catacatggc agagttatgc aatgtttctg
     2041 ctggagtcaa tgcctgagaa aacggcggag cattacaaga ataagattgc tgtttatctt
     2101 cggtggtggc agtctcatgg atggccggat ggcatccctg atgagcagga taatgaccag
     2161 ggatgtaaag atattccctc ctggcgtcgc atctgtaaga cgttactgaa aaatgatttc
     2221 tggtgtcgga tgctggcgtt tagtccgaat aagccccgac attatgagcg ctacctggcc
     2281 agaatgcgtg aaaaaaggaa agagtgggga atattgtaaa ttatatttaa acatcagaaa
     2341 tatgacggtt aatccaggat agtagctaaa aaaataagat tccttgctga tttatccctg
     2401 tttgatatgt aaaatctgag gggcttataa gcaacggata tacgagtgct acaatatatg
     2461 ataagttata tatattaaat gttcatcatc tgtcaggggc tgttttatgg ctaaactgat
     2521 aaatatatca ggtgttaccg aggagcggat gactgccggg gaaaagcggg ttgcatcccg
     2581 tcttgaggca tttatgagta atgagtgtct ggtctggtac gatattccgg tgggacggaa
     2641 aaaccgacat cctgattttg tcattattga cccggagaat ggtctggtat ttcttgaagt
     2701 caaggactgg accgttcaca cgttgcatca ggtaaatcac gaacaggtca ttcttgaaac
     2761 taacggtatt cttaaatcag aaataaatcc gctgctacag gttcgtcgtt atgcctgtga
     2821 taccgtaaat gctttacctg cagactcacg tttgcgacag aatgatggtc agtataaggg
     2881 gcgtctgaat ctggcctggg cgtatggtgt tgtgtttacc cgtattaccc gccagcaact
     2941 gaaggctctg gctgggaatg atgaaaatgc tgtggaaagg attttccctt cagcgcagac
     3001 aatctgtcag gatgatatga ctcagtctgt tttacctgat atattccgcc aaaaaattgc
     3061 cgggatgttt acgatgggat tcagaacccg ggtgacacca caggttagag atattctgcg
     3121 tgctcatctg tttcctgaaa tgaccgtcag acagcactca caaatcaaaa taatggatat
     3181 tcagcaggag attctggcca gaaatattgg tgatgggcat cgtgttattc atggggtagc
     3241 cggctctgga aaaacaatga ttcttttgtt ccgttgtctt tacttggctg aaactacccc
     3301 tggtaagatt ctggtgctat gttacaacat tactctggcc agctacctgc gggaatgtat
     3361 tgagtccagg ggactgaagt cacgggttac ggtattccat tttcatagtt ggtgcgcctc
     3421 aatgataaag cgccatggaa ttcaggtgac tgctggagga aaggattacc cggagaaatg
     3481 cttcagtgcg ctggaagatg ccgtgaacag cggaacaatt actgatactg gatacgatgc
     3541 cgtacttgtg gatgaggggc atgattttga gagtcgctgg ctggctctga tagcccgcct
     3601 gtttgataac gcgtcccgtt cactgctcct gatgtacgat gacgcacagt ccatttacag
     3661 acgggaacgg gctctgaact tttcgcttgc cagtgtgggg attcaggcgc agggacgtac
     3721 ttccgtcctg ccggtaaatt accgtaatac gaaacgcatt ctgcactttg cttatgcatt
     3781 ctcccgtgag tattttgaga agcatcagaa ccgtgaaata ccgtttgttc agccacaggc
     3841 gggcggtgaa gagggcacag agccggaaat tctgcgttgt gcttctgaga gtgatgaggc
     3901 agtgcaggtt gtgggctggc tggagaaacg ctatacactg tgcggtcact ggagtgatat
     3961 ggctgttttg tgtcctgcag agttttcagt gaaacatctg aaagaggtca tgacacaacg
     4021 cgggatacct tatgccacct gctttgacag tgaaggtaag aagaaatatt cgcgacgcaa
     4081 ggatgttgtg catttactga cctaccagag cagtaaggga ctggagtttc cgtatgttgc
     4141 ggttatcaat gcttctttcg ttcactcagg tgctgcggat gagtctgaag ttattccggc
     4201 gctttatgtt gcgttcaccc gggctacacg ggaattactg gtgacctgtt atcgtgaaaa
     4261 cagtatcagt cgtcatcttg aggattttgt ataagttgtg ttttattatt gctgatatgt
     4321 aatcattcgt tttttgataa tctaagatgg gtatttagcg agtattcaga gttgaattaa
     4381 tatcccccat gtttttgatc tgtcaatagc tgtccattac atggggtgat catatatcag
     4441 tcggtaatta ctggataaag tcacccccta aatgattact ttattgtcca tgccggacta
     4501 aagttttcct cctcaaatag ttctgttaac ccacttattt gttttaatct cagtacctca
     4561 tctgtatcca tgcccagttc actacctatt cggagatcat cccatcccat tcgggaaaga
     4621 tctcggacga tatcggagag cgcgtctatc tggtgctttc ctctggccct gttatgcctg
     4681 atggtcgagg cgatagagta agctttaaag ttattatcat gcttaatgca tgtgacaggt
     4741 aaatatcctt ttagtttttt ctctagtttt gttgtgcttt tcccaagcat ttgtctgtga
     4801 aatccgtcaa taacggtgta tttccccttt tgagctaagg tgacaacagg ttgagtgaat
     4861 ccatcaacca acagggagtg tgttaataac cttttttcgg agggagccat gacgttagga
     4921 ttatagtcgt ttgcttcaat tagattgttt tttatccata taacacaatc aacaggttct
     4981 gcgtaaaaag gactactcct atgtaactca atcttt
//
LOCUS       DAZGHF010000231         4916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGHF010000231 DAZGHF010000000
VERSION     DAZGHF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.232"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            171..884
                     /gene="ydiV"
                     /locus_tag="ACHK3A_004818"
     CDS             171..884
                     /gene="ydiV"
                     /locus_tag="ACHK3A_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO8742775.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPVNQITTLVQR"
     gene            947..2383
                     /gene="selO"
                     /locus_tag="ACHK3A_004819"
     CDS             947..2383
                     /gene="selO"
                     /locus_tag="ACHK3A_004819"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO8742776.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVALSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYASRPPDWGKRLEVSCSS"
     gene            complement(2387..2578)
                     /gene="hemP"
                     /locus_tag="ACHK3A_004820"
     CDS             complement(2387..2578)
                     /gene="hemP"
                     /locus_tag="ACHK3A_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO8742777.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2710..3756)
                     /gene="aroH"
                     /locus_tag="ACHK3A_004821"
     CDS             complement(2710..3756)
                     /gene="aroH"
                     /locus_tag="ACHK3A_004821"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO8742778.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3913..4746)
                     /gene="ppsR"
                     /locus_tag="ACHK3A_004822"
     CDS             complement(3913..4746)
                     /gene="ppsR"
                     /locus_tag="ACHK3A_004822"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO8742779.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         1216 a         1150 c         1253 g         1297 t
ORIGIN      
        1 ttccgtaaat ggattattcg tttataatca ggataatcta aaaaaaggac ccctgaaaaa
       61 gtggttaaca tggaacaaat gaaagttact ccttctgtaa ttgttatgtc ttatggcctc
      121 atccctttta gccggatact gaaaaacatc cttcgagagg gacggttacc atgaagattt
      181 ttttggagaa tctttatcat tctgattgct atttcctgcc gataagagat aaccagcaag
      241 accttgtcgg cgttgagtta attacccatt tctccagtga agatggtacg gttcgtatcc
      301 cgacgagtcg ggttatagcg caacttaccg aggagcaaca ttggcaatta ttttcagagc
      361 agttggaatt actaaaatca tgccaacact tttttattca acataaactc tttgcctggc
      421 ttaatttaac gccacaagtg gctacattat tattagacag ggataattac gcaggcgaac
      481 tattaaaata tccgtttatt gaactgttga ttaatgagaa ttacccgcat ctcgatgaag
      541 gaaaagataa tcgcgatctt ttgtccttat cgcaaatgta tccccttgtg ttaggtaatt
      601 tgggcgcagg caatagcaca atgaaagcgg tttttgatgg tcttttcacc cgcgtcatgc
      661 tggataaaag ttttattcag caacaaatca cgcatcgctc cttcgaaccg tttattcgtg
      721 cgatccaggc tcaaatttcc ccatgttgca attgcatcat cgcggggggc attgatacgc
      781 cagaaataat ggcacagatt accccctttg atttccatgc tctccaggga tgcctgtggc
      841 ctgccgttcc tgtaaatcag ataacgacgt tggttcagcg ataaccctcc tgtttgctgg
      901 tgtttaagac gagagtaacc gtctacacta tcaaatagga ggatctatga ccctgtcttt
      961 tgttacccgc tggcgcgatg aattgccaga aacctataca gcactttccc ctacgccttt
     1021 aaataatgcc cggctgattt ggcataatac cgaactcgct aacacgctga gtattccatc
     1081 gtcgctgttt aaaaatggcg caggtgtctg gggcggcgaa accctactgc ctggtatgtc
     1141 accactggcg caggtttaca gtggtcatca gttcggcgtc tgggcgggcc aactgggtga
     1201 tgggcgcggc attttactcg gcgaacaacg gcttgctgat ggcactacaa tggactggca
     1261 tctgaaaggt gctggcctga cgccttattc gcgaatgggt gatggacggg cggttttacg
     1321 ttcgacgata cgagaaagtc tcgccagtga ggcgatgcat tatctgggca ttccgacgac
     1381 ccgcgcgtta agtatcgtca ccagcgattc gccagtgtat cgggaaacgg tggagccagg
     1441 cgcgatgctg atgcgtgtgg cactaagtca tctgcgcttt ggtcatttcg aacattttta
     1501 ctatcgccgc gagccggaaa aggttcgtca gttggctgac tttgccattc gtcattactg
     1561 gtctcatctt gaagatgatg aggacaaata ccgtctctgg tttagcgatg ttgtcgcacg
     1621 taccgcatcg ttaattgccc aatggcagac ggtcggcttt gctcatgggg tgatgaatac
     1681 cgacaacatg tcgctgctgg ggctgacgct tgattacggg ccgtttggtt ttcttgatga
     1741 ttacgaaccc ggttttattt gtaatcactc ggatcaccaa gggcgttaca gctttgataa
     1801 tcaacctgcc gtcgcgttgt ggaatttaca gcgtctggcg cagacattgt cgccatttgt
     1861 cgccgttgat gccctgaatg aggccctgga cagctatcag caggttttgt tgacgcatta
     1921 tggacaacgg atgcggcaga aactgggctt catgacggag caaaaagagg ataacgcgct
     1981 actaaatgaa ttattcagtc tgatggcgcg agagcgcagc gattataccc gcacatttcg
     2041 catgctgagt ctgaccgagc agcacagcgc ggcgtcaccg ctacgtgatg agtttattga
     2101 tcgtgcggca tttgatgact ggtttgcccg ttatcggagg cgtttgcaac aagacgaggt
     2161 tagcgatatt gagcgtcagc aactgatgca aagcgttaac ccagctctgg tgttgcgcaa
     2221 ctggttggcg caacgggcga ttgaagcggc agaaaagggt gatatgacgg aattgcaccg
     2281 cctgcatgag gcgttgcgaa atcctttcag cgacagagat gatgactacg ccagtcgtcc
     2341 acctgactgg ggtaaacggc tggaagtcag ctgctcgagt taaaggttac ttggtcaaca
     2401 gcagcttgcc agcctgagtt ttacggagca gatattcttg cccgtcatga tcgataatca
     2461 gtttgccatc cggacctaac agtgtctggc tggtaattcg ccggatgggc tgcggggaag
     2521 cggtcttgtg attggcatcc gtttcaggcg tgagttttct gctatccgta taacgcataa
     2581 aaattaataa taatttatat aacaatgatt cttattatca atattatggc gggccagagc
     2641 aagcagtaat ttgttgagaa gacaacaagt tgcgcagaaa tgcccatccc gtcaggaatg
     2701 ggcacgcatt cagaagcgag tatctaccgc agaggcgagt ttttcgacca ggcgttcggt
     2761 atcttcccag cccagacacg ggtcggtaat ggattgaccg taagtgagcg gctgaccgcc
     2821 gacgattttt tgcgttcctt cgcgcaggaa actttccgcc ataattccag caatcgccgt
     2881 agatccattg cggatttgct gacaaatatc ctcacaaact tctaactggc gtcggtgctg
     2941 cttctggcag ttaccgtggc tgaaatccac caccagatgt tcaggtaaat caaactcgtg
     3001 cagcgtatcg caggctgcgg cgatatcatc ggcatgataa ttcggttttt tgccgccacg
     3061 cataataatg tggccatacg ggttgccgct ggtctgatag atggtcatct gaccattttt
     3121 gtctggcgag aggaacatat ggctggcgcg ggctgcgcgg atagcatcca cggcaatccg
     3181 cgtgttgcca tcggtaccat ttttaaaacc taccggacag gagagtgccg aagccatttc
     3241 acggtggatc tgactttcgg tagtacgtgc gccaatcgcg ccccaactga ttaaatcagc
     3301 aataaactga ccggtcacca tatcgaggaa ctcagtcgcg gtagggacgc ccagctcatt
     3361 tacctgtaaa agtaatttgc gcgccagctc cagaccgtga tttacccgat agctgccgtt
     3421 taaatctgga tcggagatta gccctttcca gccgacaaca gttcgtggtt tttcaaaata
     3481 ggtgcgcatt acgatttcca gccgtgactg gtactggttg cgcagcgact gcagacgggt
     3541 ggcgtactcc attgcagcgg tgagatcgtg gatcgagcag gggccaataa tgaccaacag
     3601 tcgcttatct tcaccattca gtattttttc aattctgcgg cgggagtcgg tgacatgggt
     3661 ggcgacgcca ggcgttacgg gataccgtag cgcgagttcg gcgggcgtta ccaggctctc
     3721 aatacgcgca gtacggagtt cgtcagttct gttcattaca agtctcagaa atttgttgct
     3781 tctacggcag gactaccggg aagtgatgcg catcacgata aaccaatcgc agctgatttc
     3841 aagtgcgagg tgtgtcacac ctcgccgggt ggttagcatg ataacaaaaa aacaagctaa
     3901 tgcactagtt ctctagtaca ttcgacggct aaggcccatg atatcgagga tcttggtggc
     3961 aatctcttct accgaataat tggtactgtt aatccacggg atctgatttt tacggtacaa
     4021 cgcttccact tccgcgactt ccatcctgca ctgacgaagc gaggcatagc gactgttctc
     4081 ccgacgttcc tcgcgaatcg ccgccagacg ttccgggtcg atagtcaggc cgaacaattt
     4141 atgctgaagc ggtttgagcg acgcgggtag cacaagatta tccatatcgt cggcaataaa
     4201 ggggtagttt gccgcgcgga taccaaactg cattgccaga tacagactgg tgggggtttt
     4261 accacagcga gaaacgccga gcaggatcac ctgagcctgg tccagattgc gtaacgaaat
     4321 gccatcatcg tgggcgaggg tgtaatcaat cgcagcaatg cgcgcatcat atttattgag
     4381 attattaggg ttaaggccat gggtacgatg agcaatcggc gttggatcca gtttcatctc
     4441 ttgttgtagc ggggcaacca gcgcctgaac gatatcctgg caaaagccct cactttgcaa
     4501 gatgatggcg cgaatctccg gcaacacgat ggagtagaag accagcgggc gcacacctgt
     4561 ctggtgataa attgcgtcaa tctgatcctt cactgcccgt gcacggctct cattttcgac
     4621 aaacggcagc gtaatgctgc tgatagtgac gggaaattgt gacattactg cgtgtcctaa
     4681 tacctccgca gttattgccg taccatcaga aatataaaaa acgtggcgat caacagcatt
     4741 atccattttg ttcttcccgt gatgcagaca taattctatg aaagcataaa ttaaaaacgc
     4801 acagaagcgt agaacgttat gtctggttta taaaatgaac cttcaatttt attttttatg
     4861 aaaacagcat ttcattttta cggtttcgtt tataccgatg gtttatgtgg aaattg
//
LOCUS       DAZGHF010000232         4894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGHF010000232 DAZGHF010000000
VERSION     DAZGHF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.233"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            90..842
                     /locus_tag="ACHK3A_004823"
     CDS             90..842
                     /locus_tag="ACHK3A_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8742780.1"
                     /translation="MIKIYKASKGQLKHREIFDEIKKKVDNGEWKEGKAIPTERELSE
                     YYLSSRTTIRKAIESLKQRGYLHSVHGQGTFVLPSRSRENHLLHSFTDDIKARGGIPA
                     QNILEIGYVPVSDVIRKNLDLDIHVQTVFCIKRVRYMGSTPLGIQTSWLALNDRQEIT
                     QEDLLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            856..1932
                     /locus_tag="ACHK3A_004824"
     CDS             856..1932
                     /locus_tag="ACHK3A_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO8742781.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWSGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALEAQEK"
     gene            1932..2930
                     /locus_tag="ACHK3A_004825"
     CDS             1932..2930
                     /locus_tag="ACHK3A_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO8742782.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEDDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            2935..3849
                     /locus_tag="ACHK3A_004826"
     CDS             2935..3849
                     /locus_tag="ACHK3A_004826"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO8742783.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEVNLFLSQHD"
     gene            3877..>4894
                     /locus_tag="ACHK3A_004827"
     CDS             3877..>4894
                     /locus_tag="ACHK3A_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO8742784.1"
                     /translation="MKIRYAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTI
                     AGLSAITFDAKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDA
                     KQQPFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNIS
                     SDKESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSID
                     ADGRPSKQLHEYLYITDPVPQVSKFGINDNGVSEVMALNDHQLLVIERSGRNVSAGFN
                     DWDYSVRVYMVDLTAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGV
                     TFGPL"
BASE COUNT         1431 a         1038 c         1118 g         1307 t
ORIGIN      
        1 tttatgtcac agatagtgaa caagcaccat gcgtaataaa agttaaagaa tgtagagttg
       61 gtatcaacca cttgaaatga ggctttgtca tgataaaaat ttataaagcc agtaaaggtc
      121 agctaaagca tcgtgaaatc tttgatgaaa taaagaaaaa agtagataac ggtgaatgga
      181 aagaaggcaa agccataccg acagaaaggg aattgtcaga atattattta agtagcagaa
      241 caactattcg aaaggcaata gaaagtctta aacaacgcgg ctacctgcat agtgtccacg
      301 ggcaaggaac atttgtattg ccttctcgta gtagagaaaa tcatttattg catagtttta
      361 ccgacgatat taaagctcga ggtggtatac cagcacagaa tattcttgag ataggctatg
      421 ttcctgtcag tgatgttatc agaaaaaatc tcgacctgga tatccatgtc caaaccgtat
      481 tctgtataaa acgcgttcgc tatatgggaa gtacccccct gggtattcag acatcatggc
      541 ttgcattaaa tgacaggcaa gaaataaccc aagaagatct gctggcgacg ggttcgctct
      601 atattttact ggaagaaaaa ctcgctatta aacctttaga agcaacagaa attatctctg
      661 cccggcttcc ctcgcccctt gagcgtcaat tactggaact gactgccgat gatgtcgtat
      721 taagttgtac ccgcgtcacg ttatccgtgg aacgcaaacc aatggagtat gtagagatgg
      781 tgtatcccgc aagccggtac tcctacaaag cacgaattac aaaagacagt tttagcgtgt
      841 aacagagaga atattgtgaa caaaatagaa caagctgtaa tttataacaa aatattaggt
      901 tctttagcct gcgcaggtat gggcgatgca ttaggcgcag cgactgaact ctacagcatt
      961 gatgaaataa aagctcaatg gggaggtttt ttaaatgcct ttgtttctcc tcctgccgat
     1021 acattcgctg gctcattaaa tggtattgcg ggacttatca ccgacgattc cagccaaatg
     1081 tatgtgtttt cagaagggtt aattgaggct ggatttgata acttcaccaa taatgactgg
     1141 ttagcttgtc ttctgcgttg ggccgatatg cagccctacg caaattataa aggccccaca
     1201 acagaacaaa tcgtcaaggc actaaaagaa ggtagaccaa caaacaccat tgggcgtatt
     1261 ggaacatctt ctcgtcaggc acccaatgtc ggcaccacga atggagcagg aatgcgtgta
     1321 gcccctgcag gattgatctg gtcaggcaag aaagaaaaag cgtgtcacct ggcgctaata
     1381 acttgcctac cttcgcatga caccaatatt gcgattgcca gcgcttgcgc catcgctgcg
     1441 gcgacatcac aagctatgtt accagaagct tcgctcacat cattactgga cgccgcaatc
     1501 tggggagcga attacggcga acgcctggcc aaacaatatg cacgatgtgt cgcagggcca
     1561 tctatcgcca tgcgcatcca acttgcagcg gacatcgcca gacgagccaa tgacctggag
     1621 tcttgtctgc gagaaatgga aggtttggtt ggtaactctg ttgccgccca tgaatccatt
     1681 cctgcggcaa tcggtctatt gctgtactgc aaaggagaac cctgggaaac cattcatgcc
     1741 tgcgccaata ttggcaatga caccgatagc atcgctacga tggcaggcgc aattgcaggg
     1801 gcatggcgcg gttttgatgc gctccctgaa gataaatatg cctttttccg cgccgtaaat
     1861 aataaagatt tcgatattga agcgatcgca tcgggtctga cattgcttgc attagaggca
     1921 caggagaaat aatgaacatg ccagcccgta ctcctgttat gccattagcg caaactcgtg
     1981 aggagataaa tgctacggcg gcatcgttac gccatttggc agctatctgg ggtgaatgtt
     2041 tttctacttt tcagctcgat aaacgtgctg ttgaacgcgt ctgtatagtg ggatcgggcg
     2101 actcctggac ggtggcacta tgtgccgccg cctggctggg gaaatacaca catctgtttt
     2161 gttacgcatt gcaaacgtgg gattttcttc agactgattt aacccgttat cagaaagaaa
     2221 cgttaatcat tattctcagt gcaagtggca gaccatcaat gaccgttgac gccctctgcc
     2281 acgcagtatg ctcaaacgcg caagtgctgg gtgtgacgaa ttgccctgga acaccatttt
     2341 gcgcaataac agaaaacatg ctctatacat gggcgaataa gcagggtatt ccaacgcaaa
     2401 gtagtagcgt cacactttat gccctcctgc gcttagcgca aaaattatgt ccaggcttaa
     2461 caccactgca gattgaagat gatttggaag ggaaatttag ccagattaat cagcactggc
     2521 agcaaaagaa gcgccatttt taccaacaaa aagagataac gtttcttggg agtggcttaa
     2581 gttggggact ggcaatatcg gggagcaatt tactttcttg cggcccacag attcgtgcga
     2641 cagcccttcc actggaagaa ttttatcatt cattacgctt acatcaggct ggtgtcggtc
     2701 agcattatat tttactacca gccacatcct gcgagtcccc attttacctt gcaacccaaa
     2761 aagcaattgt tgaacaggga gcgacagcag aattaatcag ttttatacct gacgccagtg
     2821 agtccaacaa tttatttctg gtcatgcagt ggttatatga aatgtgctgg catttaagtt
     2881 gtgattatgt cgacgcaggc gggcaacgcg ttagccatag ggagaaataa gactatgaag
     2941 aaatttgatg tcgccgcttt aggcagtgga aatatagata tttttttatc tatcccgtca
     3001 ctccccactc gtggcggtaa agttgtcggg acacgtctgg gtgaacaagc tggcggcaca
     3061 gtcgccaaca gtgcctgtgc gatgggacaa ctgggtctca atgtggtatc tgtttcatgt
     3121 gtgggcaatg atcattcagc ctcgatcatt cttgatggat ttaaaaaata tcatgtaaat
     3181 tgtgatttcg ttcaggtaat tccggaattg attgccaata ccgcgattat ctttattgat
     3241 gaattaggtg aaaaaacgtt ggtttactcg cctggtagtg atcatgagtg ggataaagaa
     3301 aaagccctgc aggcaattgc gcaaagccgt tatttttata cgatgccagc agatatcgag
     3361 aaatttcgta tgctggccga atattcccgt agccagatga caaaagttgt cgttgatatt
     3421 gagcctcata tcattgcaac acctgaacac cttgccagaa ttttacacct tgctgatatt
     3481 gcgatattta attatgacgg ttttattcgt ggctatgcaa cagagccaga ttttacactt
     3541 ctgcatgata tacaagacga gtatcaactt gacgctgtcg tggttacgct ggatgttagg
     3601 ggagtaattg cagtcaaagg caatgagcaa gtaaaaatag gtagttatac aattcccgtt
     3661 attgatacca caggcgctgg agatactttt aatggtgcat ttgtttatag cttaataaaa
     3721 aatatgccct taattgaagc actaaaattt gcttctgcca cagcagcaat aaatattact
     3781 gcattaggtg ccagaggtca tctgccgaca ccatcggagg ttaatctttt tctttcacaa
     3841 cacgactaaa atcatatata tacaaaggta aatatcatga aaataagata tgccgctatt
     3901 tttctggcat taccgctgtt atgcacaaca gcgcaggcaa acagttttct tcattatgct
     3961 ggggagttta acttccggac gggagaaaag cagaacaatg tcacaatcgc ggggctatca
     4021 gccatcacat ttgatgcaaa aaaaaatatt ttctatgcta ttaacgatag cagaaacaat
     4081 aataaagaag gagatgccag tctttataca ctaaaaatac aggtatcatc caggggcatt
     4141 gagcaagtga attttctcaa ccagcgtcca ctgcttgatg caaaacagca acctttcgtg
     4201 accaatacgg tggatgccga aggtctggcc ctgacacaca atggtaaatc attactctgg
     4261 agttcggagt taggtgctcc actgcgtctg agcactctcg atggcgtaat ggagaaagat
     4321 tttacgtctt tatttcctgc tcgctttaat attagttctg acaaagagag cagcaatggt
     4381 attcgcagcg gcaacgcctg ggagggcctg acagttacac cagatggaaa atcacttttt
     4441 atcgccgttg aaagcagcct taaacaggat gggcctattg caagtccaat aaattcaggc
     4501 acatcacgct tactgcaatt ttctatagat gcagacggtc gaccttcaaa gcaattgcat
     4561 gaatatctct atattactga tcctgttccg caagtgagta agttcggtat taatgataat
     4621 ggcgtatccg aggtcatggc gctgaatgat caccaattgt tggtcatcga acgttccggc
     4681 agaaacgtca gtgctgggtt caatgactgg gattactctg tcagagttta tatggtcgat
     4741 ttaactgctg ccagcgatat caagaatatt gacagtctgc aagattggtc caataaatcc
     4801 acgttacaac ccgtcagtaa aaaattatta attgattttg ctgattacac ttcctctgcg
     4861 gattgtattg aaggggtaac cttcggtcct ctga
//
LOCUS       DAZGHF010000233         4846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGHF010000233 DAZGHF010000000
VERSION     DAZGHF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.234"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(690..1133)
                     /locus_tag="ACHK3A_004828"
     CDS             complement(690..1133)
                     /locus_tag="ACHK3A_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742785.1"
                     /translation="MHHYKFFALIAMLLCGASFASDVYFLCNTEKGTIKLDENKGVLR
                     YTLSKDHKTEFNYESKGSDYSGFKYNHYSRFQTDYFNVSFVNSEYKYTIFSNYEDDRK
                     SSGVSVTSLNSKKESVYYCKSIGIDRLSDLSAKMACDKYSALGCD"
     gene            complement(1120..3432)
                     /locus_tag="ACHK3A_004829"
     CDS             complement(1120..3432)
                     /locus_tag="ACHK3A_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase family protein"
                     /protein_id="HFO8742786.1"
                     /translation="MMNIRYPVRKADGRDYKSYDELLTDIRKNAHGWWLLGVNRYWHG
                     GIHVGRSSSPASVLNQETPEKSVPLQFMMDGEVVAWRVNRDYAAIECYQERPLRQSGT
                     FVLVKSVYKPDEQDESSWLTLYQLYMHIAPLSEFPKRPLYRVTQKGHGVRMRKYSRHD
                     DSREIAPDVLANKHGHPRTLTQGDTLAVLQQKSFLLKQRPESFALVQRLQDGKPAGEL
                     FWVSMRPEYLEPDGQCYVCLPDWMHSALNHGVFDDVVVPPVPLKVMVKAGDAVGFLGA
                     QDLADEDNYPQIITTDYKAHIELLSLDEHVPDVVANMKGIKTGKQFIKLKLKRPLYLR
                     NGEGEESTFEQMSAITRIDAGKIIPRDATYPFTDKTGITYFQIRPHTWMHQNDVEQLS
                     QHDLAGLNFHCIEAEHTTDFTRTLDERWLIDALKSISSHFDGEKGPQFAQAKMFYDSL
                     IHNAENRRAPNPYPDKSLDQYLFGALHTNQMNIPEYARRLIVKHDSCWHSTRDDTRWS
                     SVCKSRDESPVVKMANGGFLDATHWMDKVPPFASQRSVWHFHPLEFPEILRSAEFPRT
                     PINGVLTPIEFIHFYNGDKIDDTDYEEAAKELGCEVAAIKAVAKTETGSYGSYFKFED
                     NDDYVPAILFERHHFHKYTNGKYDQFEDISNPVAGGYGATSIQYAKLVKAYTLDKKAA
                     LKSASWGKFQILASNYATAGYASPEDFVFALSKSEKNQLKAFVSFIKADRVLLRSIRT
                     KDWLSFAQRYNGPRQKGYDLKMERNYNASL"
     gene            complement(3773..3987)
                     /locus_tag="ACHK3A_004830"
                     /pseudo
     CDS             complement(3773..3987)
                     /locus_tag="ACHK3A_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742560.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(3993..4679)
                     /locus_tag="ACHK3A_004831"
     CDS             complement(3993..4679)
                     /locus_tag="ACHK3A_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO8742787.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSVASMMAQMQLGNVKALFESKPFITLSEPACGAGSMILAMVDTLNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
BASE COUNT         1291 a         1184 c         1003 g         1368 t
ORIGIN      
        1 atccaaacag ccccatactt caggttaaaa agaacaataa aaaatgatgc taaaattgca
       61 acattctcta aataaattca attaattatc taaacaaggt gaaactttca gaggaaatga
      121 gcgcggcccc ccctaataat cttaagctgg gtacagggaa gtatcgacca ttacgcagtg
      181 tcgatatcga atcgtacaaa aaacagtctt ggcgtgggat atttacacat aattattagc
      241 tcacaatctc cctatacctg tcgtgttttc tggggattcc tcagggagtg accatagatt
      301 ccgttttcca ggtgcccccc tttttctcca aaaattgaaa aataactctg tagatgccgc
      361 taacaatcaa ttcgagttga tattttaaag cgttctgtgc agaaagaaat aaatttcaaa
      421 aatgaatcag gggagaaaag tgcgcttttt aacctgttca gtgtccagaa cacatgaaac
      481 cgatcgccat tcagtttcat gcatagtcat gcaacgcact gaaaatgatt ctgacgcatt
      541 tttccggttt cccccgagga aaacatccct ttttgcgtca gaatcgcgct cagcgcgttt
      601 tagcggcacg tacaattaca ggattataaa tcgtcaccag cgactggcgt aaaaaagccc
      661 ctctctgcag gcgagattta gatgttttat taatcacacc ccaaagctga atatttatca
      721 caagccattt ttgccgacaa atcactaagg cgatcaatgc caatggactt acaataataa
      781 acagattctt ttttgctatt caggctcgta acactaactc cgctgctttt tctatcatcc
      841 tcataattac taaatatcgt gtacttatac tctgagttta caaacgatac attgaaataa
      901 tcggtttgaa atcgtgagta atggttgtat ttaaaccctg aataatcact acctttcgat
      961 tcataattaa attcagtttt gtggtctttc gataaggtgt accgaagtac acctttgttt
     1021 tcatctagtt tgattgttcc tttttccgtg ttgcataaaa aatacacatc acttgcaaac
     1081 gatgccccac acagaagcat tgcaatcaac gcaaagaact tataatgatg cattgtaatt
     1141 tctctccatt ttcaaatcat atcctttctg ccttggtccg ttatatcttt gagcaaatga
     1201 aagccaatct ttagtccgaa tagagcgcag taatactcta tcagccttaa tgaagctaac
     1261 gaatgctttt aactgatttt tctcagactt acttaatgca aaaacgaaat cctcagggct
     1321 cgcgtagcct gctgtggcgt agttcgacgc taaaatctga aacttacccc aggatgctga
     1381 ttttaaagct gctttcttgt ctaaagtata cgctttaact agctttgcat actgtattga
     1441 tgttgcacca tacccacccg caacagggtt tgatatatct tcaaactgat catacttccc
     1501 atttgtatat ttatgaaaat gatgcctttc aaataatatc gctggaacat aatcatcgtt
     1561 atcttcaaat ttaaaatagg agccataaga accagtttct gtttttgcta cagctttaat
     1621 tgctgcaact tcgcatccta attcttttgc tgcctcttca taatcagtat catcaatttt
     1681 gtctccatta tagaagtgaa taaattctat aggagttaat actccattaa taggagttct
     1741 gggaaactct gcacttcgta atatttccgg aaactcaagc ggatgaaaat gccatacaga
     1801 ccgctgactg gcgaacggag gcaccttatc catccagtgt gttgcatcca gaaatccccc
     1861 gttagccatc ttcacaaccg ggctttcatc cctggactta cataccgatg accagcgtgt
     1921 atcatcgcgg gtactgtgcc agcagctgtc gtgtttcact atcaggcgtc gcgcatattc
     1981 cgggatgttc atctgattgg tatgcagcgc accaaacaaa tactggtcca gagatttatc
     2041 gggatacggg tttggcgctc ttctgttttc agcgttgtgg atcaggctgt cataaaacat
     2101 ttttgcctgc gcgaactgcg gtcctttttc accgtcaaag tgcgagctga tgcttttcag
     2161 ggcatcaatg agccagcgtt catccagcgt gcgggtaaaa tctgttgtgt gttcagcttc
     2221 aatacagtga aaatttagcc ctgctaaatc gtgctgactg agctgctcca catcgttctg
     2281 gtgcatccac gtatgtgggc gaatctgaaa gtatgtgatc ccggttttat ccgtaaacgg
     2341 gtacgtggca tcacgtggga taatttttcc ggcatcaatt cgggttatgg cgctcatttg
     2401 ttcaaatgta ctttcttcac cctctccatt tcgcaggtac agcgggcgtt tcagcttcag
     2461 cttaatgaac tgtttgcctg ttttaatccc tttcatgttt gccaccacat ctggcacatg
     2521 ctcatcgaga ctcagtagct cgatatgggc tttatagtcc gtggtaataa tttgcggata
     2581 gttatcttcg tcagccagat cctgagcgcc gaggaatccg accgcatccc ctgctttcac
     2641 catcactttc agtggcacag gcggcaccac cacatcatca aacacgccgt gattcagcgc
     2701 actgtgcatc cagtcaggca ggcacacgta acattggcca tcaggttcca gatattccgg
     2761 acgcatcgag acccagaaaa gttctccggc gggtttaccg tcctgaagac gttgcaccag
     2821 agcaaacgac tccggccgct gtttcagtaa aaacgatttt tgttgcagta cggcaagcgt
     2881 gtcaccctgc gtcagtgttc tggggtggcc gtgtttgttt gccagtacgt ccggcgcgat
     2941 ttcacggctg tcatcatggc gggaatattt acgcatccgc acaccgtggc ctttttgtgt
     3001 aacccggtaa agcgggcgtt tcggaaattc tgacaatggc gcgatgtgca tgtaaagctg
     3061 gtacagcgtc agccaggatg attcatcctg ctcgtctggc ttgtacactg attttaccag
     3121 cacaaaggta ccggactggc gcagggggcg ttcctgataa cattcgatag ccgcgtaatc
     3181 gcggttaacc cgccatgcca ccacttcgcc atccatcatg aactggagcg gcaccgattt
     3241 ttcaggtgtt tcctgattca gcaccgaggc cggggacgat gacctcccga catgaatacc
     3301 tccgtgccag taacgattta cgccaagtag ccaccaaccg tgggcgtttt tgcggatgtc
     3361 ggttagcagc tcgtcatagc tcttatagtc tcgtccatcg gctttccgta ccggataacg
     3421 tatgttcatc atcttttctt aagtgaaatc acatatttcg atggattctc gcgcgtattt
     3481 tgggtttggg gtgtagtgga accaaaaaga cggttaagcg gttaaattga atggtcaata
     3541 acaggtaaat caatctgttg agatcaacga cgtcttttgc tccgcaaacg gctaagatga
     3601 gtacagataa tttggttatc gtaaaaaagg tggctaaaaa tttcaacctg caatgtaaat
     3661 tagccggttg atttttggct atttaaattg acctcttaac ggcgtttatt gttccattgc
     3721 tccccaaccc cctatggatt ataagccgca catacattac ttcgttctgc atatcaccac
     3781 taatctgctt aagatgtact aacaacatca tcgcctgttg gctaaacgga actataactc
     3841 ctctttcatt ttcatgccgc gatacaagta atgcacacct ttaatttctt cacgttttgc
     3901 cggaatgcgc caaatagcgt tatcaaaatc aaattctttt taacgagcaa aacgccagtc
     3961 cgaagactgg cgcagtgata aaacattctg catcatgcca cattctgctc ctgaagatga
     4021 cgcagacgat ggtcccagtt tcccaggtaa tgcccgggag tcagcagtac gcggcgtcgc
     4081 tcattacaga gcgagttacc aatcaccact tctccggcca caccacacag cgacagttgt
     4141 atatacgcca tgccggctgc cagcgggtca atatccgttg ccgacaccca catcctgtgg
     4201 tatgccggat accccgaacg gttcagggtg tcaaccattg ccagaatcat actgccggca
     4261 ccacaggcag gttcactgag cgtgataaag ggtttactct caaacaacgc cttcacattt
     4321 cccagttgca tctgtgccat catgctggca acactccagg gcgtgaaaaa ctgactccgg
     4381 tatttatccc ccagttccag ttgcatgaac acactaccca gaaaatcaca gaatccctgc
     4441 tccagtccct caatgacatg agcaagcaaa tgcgcaatgt ggataacatc ctgcttttca
     4501 taaccactga cgattcgcag atacttctgt tcccgggcct cactgaactg aagccggttt
     4561 tccagggcaa tcacactaca gctgacaaag tcttcaaaca ccttatgacg tttgtgataa
     4621 caggctgtcc ggttaaacaa cgaaatgaat tccttctgat ggtcagtcat ggtggccatg
     4681 ttttctccgt aaaaacaggg aacacatggc ccggacaggg gaacaggtgt tccccttcgg
     4741 gttgaaatgt tgataaaacg ggcatcacgg acataatacc cggaggttaa ttactgggct
     4801 gagaccagag gaaccggtaa aggtgcggct ttcgtctgtt cttcag
//
LOCUS       DAZGHF010000234         4726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGHF010000234 DAZGHF010000000
VERSION     DAZGHF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.235"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(56..817)
                     /gene="kduD"
                     /locus_tag="ACHK3A_004832"
     CDS             complement(56..817)
                     /gene="kduD"
                     /locus_tag="ACHK3A_004832"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO8742788.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(847..1683)
                     /gene="kduI"
                     /locus_tag="ACHK3A_004833"
     CDS             complement(847..1683)
                     /gene="kduI"
                     /locus_tag="ACHK3A_004833"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO8742789.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGIINIGGAGTITVDGQCYEIGHRE
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1970..3151)
                     /gene="yqeF"
                     /locus_tag="ACHK3A_004834"
     CDS             complement(1970..3151)
                     /gene="yqeF"
                     /locus_tag="ACHK3A_004834"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO8742790.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3406..4635
                     /gene="yqeG"
                     /locus_tag="ACHK3A_004835"
     CDS             3406..4635
                     /gene="yqeG"
                     /locus_tag="ACHK3A_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8742791.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1222 a         1203 c         1051 g         1250 t
ORIGIN      
        1 tacaggtata aaaaaccctg ccatgcggca gggtcataaa agtaagagaa atgaattaac
       61 gagccagcca accgccatcc acggcaatgg tataaccatt cacataatct gaagcgctgg
      121 aggcaaggaa cactaccggc cccatcaggt cactcggcag tccccaacgg ccagctggaa
      181 tgcggtcgag aatttccgcg ctacgttgtt catctgcccg tagttgttga gtattgttgg
      241 ttgccatgta acccggagct atcgcattaa cattaatgtt gtgtttagcc cattcattcg
      301 ccatcaatcg cgtcacgccc atcacgccgc tttttgatgc ggtataagaa ggcacacgga
      361 tcccgccctg gaaggagagc attgacgcga tattgataat cttgccgcca ttgccttgcg
      421 cgataaagtg tttcgccgct gcctgagaca tgaagaatac gctcttgata ttcaggttca
      481 tgacatcgtc ccagtctttt tcgctgaact cgagagcgtc ttcgcggcga atcaatccgg
      541 cgttattcac caggatatca atatgaccaa actcagctac cgcgcgatcc agcagtgctg
      601 gaatgccatc aatctttcgc agatcggcgg tcaggcttaa aaaacgacgc cccagcgccg
      661 tgacctgctt gatggtttca gtcggttcaa cgatgttaat gccaacaatg tcacagcctg
      721 cttgcgccag ccccaacgcc atcccctggc ccagcccagt atcacaacca gtgacgaccg
      781 caactttacc ttcgagagaa aatgcactta aaatcataac aataccttat tcgtttatgc
      841 ccacaactag cgcaaatctt taacggccac atggtccata tcatcaaaaa cctggttttc
      901 accgaccatg ccccagataa aggtataagc tttggttccg acgccggaat ggatcgacca
      961 gctcggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt cttgcggttg
     1021 ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat aaacttccat
     1081 ccggcgctcg tgggtgtgac acggcatggt gttccacaag ttacctggag ccagctccgt
     1141 cagccccata ctcaattggc aggtttccag cacatccggg acaaaatatt tgttaatcgt
     1201 gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt ccggtgtgac
     1261 ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact tcgccggagt
     1321 ggcggtatca atactggcaa agacaacttc ttttgcacct ttaccaacat acagggcttc
     1381 gcggtgaccg atttcatagc attgaccatc gacagtaatc gtaccggcac cgccaatatt
     1441 gataatacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt taccaacttc
     1501 cccgccaacg gaaaccgttt tagttaccgg cataatgccg ccaacaataa tacggtcaat
     1561 gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca aaaattcatt
     1621 gcgcagcccc tgggtatcca gcgtttttgc gtgcgcactg tggatgctct gtcttacgtc
     1681 cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg aaacgctgtt
     1741 tcgttttctc tataagggga tgatatgccc acgggatagg gttttcaatt aaaaataaaa
     1801 caatgtttca tttttaaggt aaggactgaa aaaagtgcag tgtcgatcac gaatatgtca
     1861 tgagaatgga aaaatcaggg agccctgaaa aggtatgtcg actcgccagc caccattatt
     1921 caacagatta atgacggctg atatttatcg gtaaggagga aatgcagagc tattcgtcac
     1981 gttcaatggt caatgccacg ccctggcccc cgccgataca aagcgtagcc agtcctttgc
     2041 gggcattacg tttcaccatt tcatgaacca gagaaacgag gattcggcaa ccggaagcgc
     2101 ctattgggtg accgagtgcg atcgcgccac cattgacatt gacacgacgc tcatcccatt
     2161 caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc tcgataagat
     2221 cgacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca tacaccggcg
     2281 caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg cgggccagca
     2341 cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattactgca gctgcgccat
     2401 cgtttatgga tgatgcatta cccgctgtca cagaaccgag actatcaaat gaaggattta
     2461 aacgggctaa gccttctgca ctggcgtcag tgcgtggctg ctcatcggta tcaacaacca
     2521 acgtctgacc gttactttgg gtcattaccg ggacgatctc atctttaaat cgtccagcgt
     2581 caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc aactgacggc
     2641 tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa tcattgaagg
     2701 catcccacaa cccatcgtgc acaagactat caaccaactg gctattgcca agctgtgcac
     2761 cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg ccaccggcga
     2821 tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt gctttaagcc
     2881 cggaaccgca aacgtcatta atagtgattg cagaaacgct attaggaaga ccacctttaa
     2941 tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga ccaagaatta
     3001 cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc acgaccaggc
     3061 tgccaagttc cacggcggaa tgacccgcta acgcaccacg gaagcagccg atgggtgtcc
     3121 gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta acagtgcaat
     3181 agtaaatgaa tgttaattac agttatctta attgtttaaa aaaagtgatt tttatcacaa
     3241 aggaaatatg cctgagtagc aatcagagac ataactggca cgtaaggttt gcaaccacta
     3301 acccaccaat agagaggtag aaagggcgtt aatctcccat acttaacctg gtttatggta
     3361 aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc taatatttgg
     3421 tcaaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg tgcaggcacg
     3481 ctcttccttc ctattcagtt aggttcggcg ggggctgtgg tcctgtttat tactgctctg
     3541 gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat cctctcatcg
     3601 aaaacatcag caggtgaagg gataacgggc gcggtaacac actactatgg caagaagatt
     3661 ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt gatatatgca
     3721 gtggcaatta ccaactcact tacagaacag ctggcaaagc atatggttat tgatcttcgc
     3781 atccgtatgt tggtgagtct gggggttgta ttaatcctga atctcatttt tctgatggga
     3841 cgccatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc ctatttctta
     3901 tttctttcta tttacctggt cggtagttgg caacctgatc tattaacaac ccaggtagag
     3961 ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt tttcgctttt
     4021 agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata tggcgaacac
     4081 gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg cataagtgta
     4141 ttgttctttg tctttagctg cctgctttct attccacctt cgtatattga agcggctaaa
     4201 gaagaagggg ttaccatttt atcggcgctt tctatgctgc cgaacgcccc agcatggttg
     4261 tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct gggtacgtac
     4321 tttggcgtta ttgaaggtgc cacagaggtc gtcaaaacaa cattacagca ggttggtgta
     4381 aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct gattaccttc
     4441 attgtttgtt gcattaaccc gaacgcgatt tcgatgattt acgcgatcag cggcccgctc
     4501 attgccatga tacttttcat catgcctacg ctgtcaacgt atctcatccc ggcgcttaaa
     4561 ccctggcgtt ccatcggaaa tctgattacc ctgatcgtgg gtatcctgtg cgtatcggta
     4621 atgttcttta gctaatatcc gttgtggatg gttcaaaatt gcttcaattt tgaaccatcc
     4681 cagttttcat aatgttcatt tgttattatt ccgctctatc acttct
//
LOCUS       DAZGHF010000235         4603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGHF010000235 DAZGHF010000000
VERSION     DAZGHF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.236"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHK3A_004836"
     CDS             complement(<1..593)
                     /locus_tag="ACHK3A_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO8742792.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(708..783)
                     /locus_tag="ACHK3A_004837"
     tRNA            complement(708..783)
                     /locus_tag="ACHK3A_004837"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Thr,seq:ggt)
     gene            complement(790..864)
                     /locus_tag="ACHK3A_004838"
     tRNA            complement(790..864)
                     /locus_tag="ACHK3A_004838"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Gly,seq:tcc)
     gene            complement(981..1065)
                     /locus_tag="ACHK3A_004839"
     tRNA            complement(981..1065)
                     /locus_tag="ACHK3A_004839"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Tyr,seq:gta)
     gene            complement(1074..1149)
                     /locus_tag="ACHK3A_004840"
     tRNA            complement(1074..1149)
                     /locus_tag="ACHK3A_004840"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Thr,seq:tgt)
     gene            1511..2461
                     /gene="coaA"
                     /locus_tag="ACHK3A_004841"
     CDS             1511..2461
                     /gene="coaA"
                     /locus_tag="ACHK3A_004841"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO8742793.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHK3A_004842"
     CDS             complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHK3A_004842"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO8742794.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHK3A_004843"
     CDS             complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHK3A_004843"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO8742795.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQSVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPSTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1181 a         1157 c         1046 g         1219 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgccccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgc
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      481 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      541 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      601 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      661 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      721 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      781 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      841 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      901 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      961 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
     1021 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
     1081 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
     1141 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
     1201 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaacgg
     1261 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgtgctc ctgctaaacc
     1321 ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata gcttcctggg
     1381 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1441 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgcctg
     1501 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1561 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1621 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1681 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1741 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1801 cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1861 acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc aggttctgaa
     1921 agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1981 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg tttactcgca
     2041 tcttatttat gatgtgatcc cggatggaga taaaactgtt gttcagcctg atattttaat
     2101 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     2161 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     2221 gacgtggtat atcaaccgtt ttctgaaatt ccgcgaaggg gcttttacag acccggattc
     2281 ctattttcat aactacgcga aattaactaa agaagaagct attaagactg ccatgacatt
     2341 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgtgc
     2401 cagtttaatc ctgacgaaaa gtgctaatca tgcagtagaa gaggtcagac tacgcaaata
     2461 atttaaaggg gagttaatgc tcccctttct tatttttctg cactacgcag ggatatttca
     2521 ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcacc ctgtttgtct
     2581 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2641 ataaaattat ccaacttttc ccagcgcgaa agataaggtg tcaatccttc ttgctcgaag
     2701 agttccagcg ccgcgcgtaa ttcacgtatc agcatggccg caagagtatt acgatcgaga
     2761 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2821 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2881 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2941 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     3001 accagactta aaccaatcgc cgccgccggg ccctgttcca gacgccagaa catcgacaaa
     3061 tataagttcg cgccaaaagg cgaaaaccat ttccgaccac gacgaccacg gccagcatgc
     3121 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     3181 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     3241 atcttttcgg cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     3301 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3361 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcgccatt cgctaacagg
     3421 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3481 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3541 ctttttgtct tacatgatgc gccagctgta caacatcttc gctttttgca tcgtgctcat
     3601 ttatgagaac cagcgcctgt tgacggtgta ccgcagcccc accaatttgc gtccctttaa
     3661 gctggcactg atcaataagc caacctgcag ccagttttac tgaaccatcc acctgggggt
     3721 aatgtggggc tgttggaaat tgtgccaaca acgcgttagc agtttcggca gaaacaacag
     3781 gatttttgaa gaaactaccc gcattaccgt tcacttttgg atcagggagt ttggtggtgc
     3841 gcatatgaca caccgcatca aatacttgct gtggcgttac cgttgaagga tccaggcgag
     3901 ttaagtcacc atacgtcagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3961 caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtctcgatag ccaaaacggc
     4021 actctttggc agttaagcgt acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     4081 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     4141 tacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc aaagtgtatt
     4201 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaggttcat
     4261 catgaatttc gatacctttg atccggttga tgatcaccgt accgcgatag tcttccagaa
     4321 aaagtacatt acttccttca cccagaataa gaacggattg tccttttgcg gttgcatgct
     4381 gccaggcatt gagtagttgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4441 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4501 aacgaggcta gtttaccgta tctgcggggg gatggcttgt agatatgatg acaggaagag
     4561 tttgtagaaa cgcaaaaagg cccagtcttc cgactgggcc ttc
//
LOCUS       DAZGHF010000236         4536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGHF010000236 DAZGHF010000000
VERSION     DAZGHF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.237"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..573
                     /locus_tag="ACHK3A_004844"
     CDS             <1..573
                     /locus_tag="ACHK3A_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8742796.1"
                     /translation="MLRLRRAGQITGQHVPEIILLNSHDGTSSYQMLPGYFRAICTNG
                     LVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQ
                     AALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTHAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            628..1113
                     /locus_tag="ACHK3A_004845"
     CDS             628..1113
                     /locus_tag="ACHK3A_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8742797.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQSQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIVACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1129..1605
                     /locus_tag="ACHK3A_004846"
     CDS             1129..1605
                     /locus_tag="ACHK3A_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO8742798.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1668..1889
                     /locus_tag="ACHK3A_004847"
     CDS             1668..1889
                     /locus_tag="ACHK3A_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO8742799.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            2052..2426
                     /locus_tag="ACHK3A_004848"
     CDS             2052..2426
                     /locus_tag="ACHK3A_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO8742800.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPAQAPATTS"
     gene            2473..2850
                     /locus_tag="ACHK3A_004849"
     CDS             2473..2850
                     /locus_tag="ACHK3A_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO8742801.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            2847..3335
                     /locus_tag="ACHK3A_004850"
     CDS             2847..3335
                     /locus_tag="ACHK3A_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO8742802.1"
                     /translation="MKLTLTLEADNVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITETDNAWLYSLSHQTNDDCETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            3347..3544
                     /locus_tag="ACHK3A_004851"
     CDS             3347..3544
                     /locus_tag="ACHK3A_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO8742803.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQVQLLHQNR"
     gene            3629..4474
                     /locus_tag="ACHK3A_004852"
     CDS             3629..4474
                     /locus_tag="ACHK3A_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8742804.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDWWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKECYEDEMFSIRYFKKGSAHITFKNQELVDRLNDIIARHYPR
                     MLPSL"
BASE COUNT         1111 a         1239 c         1149 g         1037 t
ORIGIN      
        1 atgctgcgtc tgcggcgggc cggacagata accggtcagc atgtgcctga aattattctg
       61 ctcaactccc atgacggtac ctccagctac cagatgttac ccggatattt tcgtgccatt
      121 tgtaccaatg gcctggtctg cggtcagtcg ctgggagaag tccgggtgcc acaccgggga
      181 aacgtggtgg acagggtcat agaaggcgct tacgaagtgg tgggcgtgtt tgaccggatt
      241 gaggaaaagc gtgatgccat gcagtcgctg gtcctgccgc caccggcacg ccaggcgctg
      301 gcacaggcgg cactgactta ccgttatggt gacgaacatc agcccgtcac caccgccgac
      361 attctgacgc cacgacgccg ggaggattac ggtaaggacc tgtggagtgc ttatcagacc
      421 attcaggaga atatgctgaa aggtggaatt tcaggtcgca gcgctaaagg aaaacgtatc
      481 catacccatg ccattcacag cattgacact gacattaagc tcaaccgcgc attgtgggtg
      541 atggcggaaa cgctgctgga gagcctgcgc tgattcagcc attaccctga aaaccacgca
      601 tttatttgtc ctttaaggag tcatttcatg aaaaccattt cacataattc caccacatct
      661 tctgtttccg taaccgctgc atcagggaat gaccagtctc agctggttgc cactcccgtc
      721 cctgatgaac agcgcatcag cttctggccg cagcattttg gcctcattcc acagtgggtg
      781 accctggagc cccgtatctt cggctggatg gaccgtcttt gcgaagacta ctgcggtggt
      841 atctggaatc tgtacacact gaacaacggt ggcgcattta tggcccccga accagatgac
      901 gatgatggcg aaaactgggt actgttcaat gccatgaacg gtaaccgcgc tgaaatgagc
      961 ccggaagccg ccggcattgt cgcctgtctg atgacgtaca gtcatcatgc ctgtcgtacg
     1021 gagtgttatg ccatgacggt ccattattac cggttgcggg attacgccct gcagcatccg
     1081 gaatgcagcg ccattatgcg catcatcgac tgaatggaga gaagcacaat gcaacaactt
     1141 tcctttctgc ccggagagat gacgcccggc gagcgcagtc tcattctgcg ggccctgaaa
     1201 accctggacc gccatcttca tgaacccggc gtggccttca cctccacccg tgcggcacgg
     1261 gaatggctga ttctgaacat ggcgggactg gagcgtgaag aattccgggt gctgtatctg
     1321 aataaccaga atcagttgat tgccggtgaa accctcttca ccggcaccat caaccgcacg
     1381 gaagtccatc cccgggaagt gattaaacgc gccctgtacc acaatgccgc tgccgtggtg
     1441 ctggcgcaca atcacccgtc cggtgaagtc acacccagca aggcagaccg ccttatcacg
     1501 gaacgtctgg tacaggcact gggcctggtg gatatccggg tgccggacca tctgatagtc
     1561 ggtggcaacc aggttttctc ctttgccgaa catggtctgc tttaacccgt cacaaccaca
     1621 tcacgcttgt tttcactttt atcttctgtc ttcagaggta tcacactatg aaaatcatca
     1681 cccgtggtga agccatgcgt attcaccaac aacatccggc atcccgtctt tttcctttct
     1741 gtaccggcaa ataccgctgg cacggcagca ctgaagcgta taccggtcgt gaagtgcagg
     1801 atattcccgg tgtgctggcc gtgtttgctg aacgccataa ggacagtttt ggcccgtatg
     1861 tccggctgat gagcgtcacc ctgaactgat gttccgtcac ccggaaaacg agtacgacca
     1921 gatggactca gacgccgctg tggatgaggc cctgctcatc aacgaataca cctttaccga
     1981 agcaggtcag cgcgccggct gacctgtcat cgttaaacca ttcttaacca cttttcagag
     2041 aggattttat cgtgtcagac aaactctccg ggataactca tcccgacgac aatcacgacc
     2101 gcccctggtg ggggcttccc tgcacagtga ggccctgttt tggtgcccgt ctggtgcagg
     2161 agggtaaccg cctgcattac cttgccgacc gcgccggtat cagaggccgc ttcagcgacg
     2221 cagatgcata ccatctggac caggcctttc cgctgctgat gaaacaactg gagctcatgc
     2281 tcaccagcgg tgaactgaat ccccgaaatc agcataccgt cacgctgtat gcaaaagggc
     2341 tgacctgcga agccgacacc ctcggctcct gtggttacgt ttatctggct gtttatccgg
     2401 cacaggcacc cgcaaccacc tcataacccc gaagctctca cctttagcac taccagttac
     2461 agagaactta agatgaaaac attatccgac acacatgtac gggaggtatc tcgctgcccg
     2521 tctcccgtca ccatctggca gacactgctc atccgactgc tggaccagca ttatggcctc
     2581 acactgaatg acacaccgtt tgccgatgaa cgtgtgattg agcagcatat tgaagctggc
     2641 atttcactgt gcgatgcagt gaactttctt gttgaaaaat acgcactggt gcgtaccgac
     2701 cagccggaat tcagtgcctg tacccgttct cagttaataa acagcatcga tatcctccgg
     2761 gctcgcaggg cgaccggcct gatgacccgc gacaattaca gaacggtaaa taacattacc
     2821 caggggaagc atccggaggc gaaacaatga aactgactct gacgctggaa gcagacaacg
     2881 ttaacgtgca ggcgctgaac atgggacgaa ttgtcgttga cgtcgatggt gttaatctcg
     2941 ctgaactgat taacgtggtc tgcgacaatg gccactccct tcgtgttgtt gatgaatctg
     3001 accgggtctc aacagactgc acgccaccat ttgctgccct taccggcata cgctgcagta
     3061 ccgcacatat cacggaaacg gacaacgcct ggctgtactc gctgtcacac cagactaatg
     3121 acgactgcga aacagaatgg attcatttta caggcagcgg atatctgtta cgtaccgatg
     3181 cgtggtcata cccggttctg cggcttaagc gcctgggcct gtcaaaaacg ttccgtcgtc
     3241 tggttgtcac acttacccga cgttatggcg tcagtctcat tcatctggat gccagcgctg
     3301 aatgcctgcc gggtttaccc actttcaact ggtaaccagg aacaacatga aatcattaac
     3361 cacggaaaca gcactggata ttctgattgc gtggctgcag gacaatatcg actgcgaatc
     3421 gggaattatc tttgacaacg atgaggataa aacggattcg gcagcactgc tgccctgcat
     3481 tgaacaggcg agggaggata tccataccct gcgccaggtg cagcttctgc accagaaccg
     3541 gtgagtctta ctcaccagac ttcattccac tgacgccagc ctgaacaggg ctggcgtttt
     3601 catttatctg cgaaaaggaa tatcgattat gtctgcaatc acagtctccc gtccggaagt
     3661 ggtcaccggg catacggacg ttatctgctc cacctcagtc agtcacattc tggctgtgcg
     3721 aaagactaca ctgctgcaaa tcgacacact tatccggcaa ctggctgaaa tctcggtact
     3781 gacagaaagt attggaggta aaactgctct ggactgggcc atgaaacagg attttcgctg
     3841 cggctgctgg ctgatggaga aacctgaaac cgcaatgaaa gctatcacac gcaatctcga
     3901 tcgtgaaatc tggcgtgacc tgatgcaacg ttccgggatg ctttccttaa tggatgcaca
     3961 ggcccgtgat acatggtacc ggtcactgga atatgataat tttccggaaa tcagcgaggc
     4021 gaatatcctg agcacatttg aacaactgca ccagaataag gatgaggtgt ttgagcgagg
     4081 ggtgatcaac gtcttcaggg gactgagctg gaattacaaa accaattgcc cctgcaaatt
     4141 tggcagtaaa attatcgtca acaacctggt gaggtgggac tggtgggggt ttcatcttat
     4201 caccgggcaa caggcagatc gacttgccga tctggaaaga atgctacacc tgttcagcgg
     4261 caaaccgatc cccgacaacc gggaaaacat cacccttcgt ctggatgatc acatccagtc
     4321 tgttcagggt aaagagtgct atgaagatga gatgttcagc atcagatact ttaagaaggg
     4381 ctccgcgcac atcacgttca aaaatcaaga actggttgac aggctgaatg acattattgc
     4441 aagacactat ccgagaatgt taccttcatt ataaagttct aagaatgaat tcaattttgc
     4501 attttagctt cattccctca cgcaaaatta tttggg
//
LOCUS       DAZGHF010000237         4445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGHF010000237 DAZGHF010000000
VERSION     DAZGHF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.238"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            966..1532
                     /locus_tag="ACHK3A_004853"
     CDS             966..1532
                     /locus_tag="ACHK3A_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO8742805.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMLARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWIDRQRVKKIY
                     PFAERIAECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRNLQK"
     gene            1790..2365
                     /locus_tag="ACHK3A_004854"
     CDS             1790..2365
                     /locus_tag="ACHK3A_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742806.1"
                     /translation="MNAIPYFDYSLAPFWPSYQSKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVLLDSCSPDCEDILKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGLF"
     gene            2434..2670
                     /locus_tag="ACHK3A_004855"
     CDS             2434..2670
                     /locus_tag="ACHK3A_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8742807.1"
                     /translation="MLTSITGHDSILLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            2952..3464
                     /locus_tag="ACHK3A_004856"
     CDS             2952..3464
                     /locus_tag="ACHK3A_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742808.1"
                     /translation="MQITKALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHRGATIRCLLLLSQTSICH
                     PRASVTVDEECSQLVDLLQQTWKVISAGGQCRVEKRFQVVRGDTSGQYVALKTVVQSF
                     VLPVMTTINW"
     gene            3553..4176
                     /locus_tag="ACHK3A_004857"
     CDS             3553..4176
                     /locus_tag="ACHK3A_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8742809.1"
                     /translation="MRTRIDYLANKYSFTELNESPRLRWQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQLIAALREEWDISQKNVMFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNESTSSPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRQSGMPVVTTSVEVSDNLTGTTRKIPAYHL"
     gene            4272..>4445
                     /locus_tag="ACHK3A_004858"
     CDS             4272..>4445
                     /locus_tag="ACHK3A_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742810.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALN"
BASE COUNT         1093 a         1032 c         1043 g         1277 t
ORIGIN      
        1 atacaatctc tattgttatt tatttcctgt cttcactcac tctcaatctt tacacattcc
       61 attagtatta ctcttgttat cgtatttact ccatccctca atcataatta ctgtaactca
      121 tatgatactc agataagtta ttctctacta cgctgttgct gatatccatc tgttatcagt
      181 cttcactgca acagtaattg attttaatct tatctatcat gaactgtatt gacttaacga
      241 tgattgttta tctgaagtgt tttaactgtt cttgtgaaca tcaattctct gtcatcactg
      301 attaactgaa tgcttacgct ttgatacggt atccatgatt ccgtcatgtt taacagcgca
      361 ggataaacaa tgaaattaac agagtgaatt cctgattata tttgttcgtt tctgattatc
      421 ttgtatgtta aacaaaatga aaattatcca tcaatggtat attttattct ggtaccgtgt
      481 acccgttaac gttcatatct tgaacatgag ttgtttttaa ccgattaaat gagtgcgtgt
      541 aagtttattg ggttacaggt atatgttggc cttgtacacg ttttttcaga ctcactgaga
      601 gcaagtttgt agtttatgat gttattggtc gtaatatgtt ttttgaacta ttggtcgtaa
      661 tatgtttttt gaactattgg tcgtgggttt ttatcgcgtg ttttgcagta tttcgtgatg
      721 tatgtttatt gattctgtat tttattcgct cctcgtttat ctcatctctt tagctaatac
      781 catcagataa tccatttctt tctgcataat gctgcgtatc gttaataacc cgtcgtatcc
      841 attctgctac agcatgcctg ataaatacca tctgtaagtt attaccgttt tagatctgat
      901 tatgagcgaa agcattaatt cgttcacaga gcttaaaaca tcattaactt tcaggagtca
      961 tcaacatgcc taaatcttac acaccaaact ggttttttac cgctttactt gacaatcaca
     1021 tcaatcaaat gctggcacgt tattcctgcc tgcgagcttt acggatggat ttcttctaca
     1081 ggaaagatac gcctgatttc ttacaacctg atcatcgctg gcttgaattg cagttgcgta
     1141 tgctgctgga gcaggtggaa caatttgaaa atatcgttgg cttcttctgg gtgattgaat
     1201 ggacggctga tcatggtttt catgcacatg cggttttctg gatcgatcgt cagagggtta
     1261 aaaaaatata tccctttgcg gagcggatag ctgaatgctg gcggtctatt acgcataaca
     1321 gcggttcggc acaccgctgt acatatcagc cgcattatac atacaacatc aacattcctg
     1381 tgcgccacaa cgatcctgaa agcatcgata atattcgcgg tgccctgcat tatctggcga
     1441 aagaagaaca aaaagacggg ctgtgtgcct acggctgcag tgaagttcct gagcgtcctg
     1501 ccgcagggcg tccacgtaat cttcagaagt gaagcttaaa ccttgagttt cctggcccgg
     1561 aaacactccg ttagtaaaaa cttaccgccc tgcttaatga tgtgaaccga agtcaatgga
     1621 gatcattcac cctgaaccac atccggtgtt ttgttccttg tcagttacct gatcttcccg
     1681 ttttgccccg gtttttcact tattccatca accccatccg caaaccataa cacgtcagct
     1741 tattcacggg caggacgcat tgtgggctgc gcataatgga gcatatctta tgaatgctat
     1801 cccttatttc gactatagcc tggcaccctt ctggccatct tatcagagca aagtcatcgg
     1861 cgttcttgag cgtgcgctgc gtgagcagtc cggctcacgg atacggcgga tcctgcttcg
     1921 tctgccgtgt gaatatgaca ataccttcaa cagcagaacg acctggtttg gtatggattt
     1981 tatcgaaacc gtcagtgcgc tgatgaatgc gaaacccgga cgcgaccttt gctggctcct
     2041 gacccgtcat cctgaaaagc cggaatacca cgtggtgctg tgtgtcagac aggagtattt
     2101 cgacggccct gaactggatc ggctaattct ggatgcctgg agcaacgtac tgggcttcgc
     2161 gtcaccgggt gaagcagcac cgtaccagaa gcagatcacc agggatgtac tgctggacag
     2221 ttgttcaccg gactgcgaag acatcctcaa agacttgatc tgggcgttca gtgatttcgc
     2281 ccgcgatcgt cgtggtgtgt gcgatccgga agcccgttgc ctcgccggta atcccggcta
     2341 tcccggatct gcaggactgt tctgaaagct cagttcagca gattcaccag catttgccca
     2401 acctccataa atctcacaga ggacatcctg actatgttga cctcaataac aggccacgac
     2461 agtatattgc tgcgtgccga cgatcccctg atcgacatga actacatcac cagtttcacc
     2521 ggtatgaccg ataaatggtt ttacaagctg atcagtgaag gtcattttcc taaacccata
     2581 aaactgggtc gcagcagccg ttggtacaaa agtgaagtgg agcagtggat gcagcagcga
     2641 attgaggaat cacgaggagc agctgcatga aacgtgttgt gatgccggta cgctggcaat
     2701 gtgcaaaatg tcggcgctgg tattgtggag atcagccctg ctcctggtgc tggcgctgac
     2761 aactcccgct catctttctg ctgactcctc tggtcagcca actgtttgtc atcatttcct
     2821 gactgattcg tcattccctt cgtcccgcca gtcactggtg tttgtatcgt atcgtgccgg
     2881 tgttgatgtg cgctttccgt gtgtctgcac cggcttaaca aaattcaaca gggtttgaaa
     2941 aggaacattt cgtgcaaata accaaagcct taatttcaga gccgggagac atccggcgtt
     3001 ttgttcagca ggcggtggac cactggccaa atctgttggc atttcacttc acgctctatt
     3061 cggcagaagg aaacatcaac gggcaacaga ttcatgcatt ctgtacttcc ttttaccgac
     3121 aggtgcacga acgtattact gagcgtaatc acacggccag tccatcctcg ccggtggtat
     3181 tacgctggtt gcgggaacaa catagaggag caacaattcg atgcctgttg ctgctcagcc
     3241 agacgagtat ttgtcacccg cgagccagtg tcacagttga tgaagaatgt tcacaactgg
     3301 tggatttact acaacagacc tggaaggtga taagcgctgg cggacaatgc cgggtggaaa
     3361 agcgttttca ggtcgtccgg ggtgatacat ctggccagta tgttgcgtta aaaacagtcg
     3421 ttcaatcctt tgtgttaccg gttatgacaa caatcaactg gtgatggtcg tgtgaaccag
     3481 ctgcgcttcc gttgattctt atggactgat aaagttttgt cagatgaatt tttatacaga
     3541 attcttttca gtatgcgtac acgaattgat tatctggcga ataaatacag ttttacagag
     3601 ctgaatgaat ccccccgtct tcgctggcag tggcaggatg ttctggagga gtgccggcag
     3661 acagaggccg ggccagaaga acggctgcgt attgctctgc tgaatgtgga ttacgtcacc
     3721 agttttgaac tgccttttcg cttgttgctt atccgtacac cacaactgat tgccgcgctg
     3781 cgtgaagaat gggacatcag ccagaaaaat gtgatgttca acgataaacg ctttggctgc
     3841 gtgtacagcc tgaaggccag cctttccggt gtgccggata cataccggta tcatctgtct
     3901 caccgtattc gtcgcgtggt tggaaatgaa agcacatcat cgccatatca gcaggttgcc
     3961 agggaagtga aagcgccccg tgaacggctg aagtatgcgc tggaggcggg cttactggtg
     4021 accgcactgg acggcctttt ctggtctggc agtcagcgta ttgcggctga tgtcctgaga
     4081 ctaagacaga gcggaatgcc ggtggtgaca acgtccgtgg aagtgagcga taacctgacg
     4141 ggaacaaccc gcaaaatacc ggcataccat ctctgatacg accatcaggg ggaatattgc
     4201 tctggcagca gcagtacgac attctttata ctttatcccc gtgcctgaaa acatgtgcta
     4261 aggacacggg aatagcaagg tccgattacg atattatcaa tctgtctccg gaacatgaac
     4321 tgaatgagtg gctggcagag agaggttatg ccggactggc ggataaccgg aaccgcctgg
     4381 cagaggtggt tgcctgcaaa ttgcaggaca gcttctgtat gaacgtttcc tgggatgctc
     4441 tgaac
//
LOCUS       DAZGHF010000238         4409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGHF010000238 DAZGHF010000000
VERSION     DAZGHF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.239"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..1123)
                     /locus_tag="ACHK3A_004859"
                     /pseudo
     CDS             complement(1..1123)
                     /locus_tag="ACHK3A_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            complement(1389..3257)
                     /locus_tag="ACHK3A_004860"
     CDS             complement(1389..3257)
                     /locus_tag="ACHK3A_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742811.1"
                     /translation="MGMFNYKIYNAAHVTYLVSSSYKLAIYSNTGKVLGLGSFLASKL
                     FAKHPDKIDYSPDALNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAISNG
                     LSGEDVIVTGYSLGGGFTNIMARLSESLADGFYANSDYIAWASPLIFDGDGKVLNIGF
                     ENDPVFRVLGDESGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPFS
                     VLALPLGSWSAHFAGSKTDAFERILGSQFYDYMSPDSTVIVDEQSGWTSWYSWVHDKG
                     DDSRERPVFIIGNDGNNLLAGGQGGDYIEGAGGNDKIEPGKGANRVDGGSGYDTLLLG
                     DVMDDWNIYRVSDGTLFFHSERGAGLTEATGIEGISFSGDWRSKLSPYQIEEEQITDH
                     RYLLKWRNKNVDYGKHTEGTNGDDSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGND
                     YLHGASGNDELYGAEGNDWLYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKG
                     EKDFLFLSKDIVTSTDEFISCLKQTGNDVEAGIAGGQIYFVNTALEDINHNSVYIV"
     gene            complement(3336..>4409)
                     /locus_tag="ACHK3A_004861"
     CDS             complement(3336..>4409)
                     /locus_tag="ACHK3A_004861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742812.1"
                     /translation="KADAEEAAEARVFYEKAFSNVYQTDDLYARTDTTSLFAPAAIKL
                     AKSTAQWATILEKNAGAQMSQDQNAGGETRYIYNGISGSDVITVGKSLGGTGLNMTAT
                     RNDMKVMTGDGDDIIITGQDYGRLASAGQWDYKYLTEMGNGNDTLIVGASNSNLNVIM
                     FNDGSIAAVKKDGAQLGSVIPFDSAYDTADGGNISGTTIDMGSGNDTVLALGHENGGT
                     AIINSTIKLGAGNDTIQINGDVKGGKSPSVITGDAGMDTLIISNGSVYSEYFSGFENI
                     ELGSKGEVKIVAADLVGKDSNSIQGGMLKIIGNSDSKVDLDGSDWIKGEIKNEGDITY
                     NVYTHASAPNISVLIEDKITQVI"
BASE COUNT         1242 a         1191 c          770 g         1206 t
ORIGIN      
        1 tcgttaaagc gatatcccat gcccaccgca acagcctgct tgtcactgta cccgcccaca
       61 gccgctgtgg cgttaaattt acccacactg tatggctgga aaagaccggc cagcgctgct
      121 gactgggctg ctgcccgctt catttcctca tgattctcat taatctgacg ctgctgactg
      181 ttcatacgac gctcatgcgc ctgcagggta cgtgcctgag aagcaatggc gcgactgttg
      241 cgttcaccca cctgacggtt acggcttacc gcagcacggt tttctgtggc catttcctgt
      301 gtacgtacca gcccttcctg tacagtaatt cgttcaccgg ttccctcacc gcgcgtcaca
      361 taaccatgac tgtccacagc aacggtactt ccggtattta tctgctgttg ctggctgttc
      421 aggcgctctt catgcgtctc cagcacgtct ctctgggaaa gcagcccctg gtgttacggt
      481 ttatggccac cgtgttccgg ataatattct ccctgttctc tgcagcaacc gtatcggtat
      541 ggtcacgcgc cttaagtgct tccgctgcaa tccgtccact gacgtcactg tacacctcat
      601 ccgccttccg gttcagttgc ccgacagtgg ctgcatccgt atcccgtgta ccatcactga
      661 caccaccgag aacccgggtt ttatccggta cacgcggact ggccgggttt gtgacacctg
      721 agtcatatcc gatgtagact tcattttcac gggttgtacc ggactgctga ccaagagcca
      781 ctgaatacct gtgcattgcc tgtgcacccg tacccagtgc tactgaattt aaagcatctg
      841 cagatgccca gctacctact gccacaccat tagacgcctc cggatttaca taagcagaca
      901 tccccacgga tgtggaaaac ggcgcatgcg catacgatgc agagccaaga gctgtccctg
      961 ccttcccggc attcgcccat gcccccactg ccgtggagtc agtctctgat gcagaggctg
     1021 tgtccggaga cgcccaggaa ctgccatcct gcgcaaacgc cggagcaccg gcaatcagaa
     1081 aggaaataag cgataaagta ataccgtgtc gatttatttt cataaacaac ctcattgata
     1141 tacacaatat actggcacag agttaaatta atatataatc aggagagaag tgaaagagta
     1201 cccggtgcga aaatgaatta atctgagcag atttaacggt aaacgaagcg caataaatta
     1261 aaattaaaaa acaaaatcac ttgcagtcta ttatctttac gttcctcacc gcaaccgcac
     1321 acaaaaacaa aacacacaat gcattgattt taaacaaaaa aacaaaacag aaactcaaca
     1381 cataaaaatc agacaatata aacagaattg tgatttatat cctcaagcgc tgtattaacg
     1441 aaatatatct gtcctcctgc aatacctgcc tcaacatcat ttccggtttg tttcagacat
     1501 gaaataaact catctgtcga tgtaacaatg tcctttgata aaaacagaaa atctttttca
     1561 cccttatgtt tattaaaatc catgataaat gaattccctt cacaagcagc atcaaagacg
     1621 aagacatcat gaccagtacc accatacaga taatcattac ctcccccacc atataaccag
     1681 tcgttacctt ctgctccgta caactcatca ttaccagaag ccccatgaag ataatcattc
     1741 ccgcgacctc cgtgtaaaag acttactcct tctcccccgg cactatgtaa tatatcattt
     1801 ccgtcaagac caaaaataat atttccgaac agactatcat ctccgtttgt accttctgta
     1861 tgttttccgt aatcaacatt tttattacgc cacttcagaa gatacctgtg gtctgtaatc
     1921 tgctcttctt ctatctgata agggcttagt ttactcctcc agtcaccaga aaaagagata
     1981 ccttcaatac ctgttgcctc agtaagaccg gctccccgtt ctgagtggaa aaacagtgtc
     2041 ccgtcactga ccctgtatat attccagtca tccattacat cgccaagaag aagcgtgtca
     2101 tacccactgc caccatcaac cctgtttgct cccttacctg gttcaatctt atcattccct
     2161 cctgccccct ctatatagtc accaccctgc ccacctgcca gcagattatt gccatcatta
     2221 ccgatgatga atacaggtct ttcacgactg tcatccccct tgtcatgaac ccatgaatac
     2281 caggatgtcc agccactctg ctcatccaca atgactgtac tgtccggtga catgtagtcg
     2341 taaaactgag accctaaaat tctttcaaag gcgtcagttt ttgaacccgc aaaatgcgca
     2401 ctccagcttc ccagcgggag agcaagcaca gaaaagggga taatattcca gaggaatgaa
     2461 ccataaaggt ctgtgtataa aacaatgtta tcaggagaag agtcatattc acgatccggg
     2521 gttgtcaagc ccggccccat tgctttaact gcctcccaga acccggattc atctcccagc
     2581 acacgaaata ccggatcgtt ttcaaaaccg atatttaata ccttcccatc cccgtcaaat
     2641 atcagcgggg aagcccaggc tatatagtca gaattagcat aaaaaccatc agccagagac
     2701 tcactgagtc tggccataat gttagtaaac ccgcccccca ggctatagcc tgtgacaata
     2761 acatcttcac cacttaaacc attactgatt gcaaagtctc tgatactttg aagcaggttt
     2821 tctgcttcag acagcgcttt tccattattg agctgtgtat aatcaacaat atcgcagata
     2881 tcatttgttc cacagaatga tattgcaatt ttctgtagat tctgttgagc atcaaacaac
     2941 ccaacaacct tcaactgagg accaaatcct gacgcagggt ttcctgtaac cggactggga
     3001 aagcgataaa aacctcttga atcgacatat tccgaagata acctgagttc ttccactgta
     3061 atatcacgcc agccttcatt aagagcatca ggagagtaat caattttgtc tggatgcttc
     3121 gcaaatagtt tagatgccag aaaactgccc aatccaagaa cctttcctgt gttgctatat
     3181 atagcaagct tataagaaga agaaacaagg tatgttacat gcgcagcatt ataaatttta
     3241 taattaaaca tccccataca gactccttta aattaccaaa tctatatatt aagaggccac
     3301 gcatacagcg cggccttgtc actatttagc tcaaatcaaa taacctgagt tatcttatct
     3361 tcgattaaca cgctgatatt aggtgcagat gcatgagtat aaacattgta agttatatcg
     3421 ccctcgttct tgatttcacc tttaatccag tcacttccat ccaagtcaac cttagagtct
     3481 gaattaccta tgattttcaa cattcctccc tgaatggagt tactatcctt tcctacaaga
     3541 tctgctgcta ctatttttac ttcaccctta ctacctaact caatattctc aaagccagaa
     3601 aaatattcag agtatacact accattacta ataatcaatg tatccatacc agcatcacca
     3661 gtgatgacag aaggagattt acctccctta acatccccat tgatctggat agtgtcatta
     3721 cctgctccaa gttttattgt tgagttaata atagcagttc caccattctc atgccccaag
     3781 gctaaaacag tatcattacc acttcccata tcaattgtag ttcctgatat attacctcca
     3841 tcagctgtgt cataagcaga atcaaaagga attacactcc ctaattgggc accatctttt
     3901 ttaactgcag caatactacc atcattgaac ataattacat tcagattaga gtttgatgcc
     3961 ccgacaataa gagtatcgtt tccattcccc atctctgtga gatatttata atcccactga
     4021 ccagcacttg ccagacgacc ataatcctga ccagtgataa ttatatcatc gccatcacca
     4081 gtcattacct tcatgtcatt acgcgtcgct gtcatattta agcctgtgcc acccaagctt
     4141 ttgcctactg taataacatc acttccggaa ataccgttat atatgtatcg tgtttctccc
     4201 cctgcattct gatcttgaga catctgagct ccagcattct tctcaaggat tgttgcccat
     4261 tgggccgtgg atttagccaa ttttattgcc gcaggtgcga ataatgatgt tgtgtcagtt
     4321 ctagcgtaca gatcatcggt ctgatacaca tttgagaatg ctttctcata aaagactctg
     4381 gcctctgccg cttcctctgc atccgcctt
//
LOCUS       DAZGHF010000239         4390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGHF010000239 DAZGHF010000000
VERSION     DAZGHF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.240"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..513
                     /locus_tag="ACHK3A_004862"
                     /pseudo
     CDS             1..513
                     /locus_tag="ACHK3A_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
     gene            754..1719
                     /gene="lipA"
                     /locus_tag="ACHK3A_004863"
     CDS             754..1719
                     /gene="lipA"
                     /locus_tag="ACHK3A_004863"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO8742813.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1820..2023)
                     /gene="tatE"
                     /locus_tag="ACHK3A_004864"
     CDS             complement(1820..2023)
                     /gene="tatE"
                     /locus_tag="ACHK3A_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO8742814.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2152..2940)
                     /locus_tag="ACHK3A_004865"
     CDS             complement(2152..2940)
                     /locus_tag="ACHK3A_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO8742815.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARVLDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFALPQLL"
     gene            3033..3415
                     /gene="crcB"
                     /locus_tag="ACHK3A_004866"
                     /pseudo
     CDS             3033..3415
                     /gene="crcB"
                     /locus_tag="ACHK3A_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
     gene            complement(3469..3678)
                     /gene="cspE"
                     /locus_tag="ACHK3A_004867"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3469..3678)
                     /gene="cspE"
                     /locus_tag="ACHK3A_004867"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO8742816.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3853..4389)
                     /gene="pagP"
                     /locus_tag="ACHK3A_004868"
     CDS             complement(3853..4389)
                     /gene="pagP"
                     /locus_tag="ACHK3A_004868"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO8742817.1"
                     /translation="MFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQPEHYDLYI
                     PAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIA
                     GYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYDPVTFQMTYI
                     PGTYNNGNVYFAWMRFQF"
BASE COUNT         1153 a         1128 c          988 g         1121 t
ORIGIN      
        1 taatcaaatt gaaccctgtt taagcagaaa atcgtcagaa ggtaaaccac aaatatttac
       61 aaccttacga aatattgatc ttacccttct gactattttg aagttgcata tgtccataaa
      121 ggaatcgtta atgcagcgaa agtgcttaat ctgcccccct cggcaatcta tcagcctatt
      181 cagaaactgc gcgttatatt ccctgaccta ttgtttattc gcaaaggcca gggtgtcact
      241 cctaccgcat ttgcgatgca tctatatgag tctatcactc agggacttga gtccattctt
      301 ggcgcgctgg atatcgaagg aagctatgat aagcaacgga cgataactat tgcgccctcc
      361 ccctcggtcg gggcccaggt ccttcctgtc atctatcggg cgattaaaac tcactatcca
      421 cagcttttac tgcgcaaccc gcccatcggc ggcgcggaaa accaactctg tcagtttcaa
      481 accgatctca tcaccgataa catgttttgc acaggaccat agatgttatc cgtaatgcat
      541 tttgaaaaag tattgttaca gccagatcac agcgcagaac gataaaatgg cacaatccaa
      601 caacaatttt acattttcgc gaccgctttg gctgctttca ggtccgtttc aatgatatac
      661 tgccggtcgt taattcaaaa atagttgata attacaacaa tctattgaat tgaaacgctt
      721 tccttcgtaa ttcgcaactg gaacacgcac gctatgagta aacccattgt gatggaacgc
      781 ggtgttaaat accgcgatgc cgataagatg gcccttatcc cggttaaaaa cgtggcaaca
      841 gagcgcgaag ccctgctgcg caagccggaa tggatgaaaa tcaagcttcc agcggactct
      901 acacgtatcc agggcatcaa agccgcaatg cgcaaaaatg gcctgcattc tgtctgcgag
      961 gaagcctcct gccctaacct ggcggaatgc ttcaaccacg gcacagcaac gtttatgatc
     1021 ctcggcgcta tttgtacccg ccgttgtccg ttctgtgacg ttgcccacgg tcgcccggta
     1081 gctccggatg ccaatgaacc agtgaaactg gcgcaaacca ttgccgatat ggcgctgcgt
     1141 tatgtggtta tcacctccgt tgaccgtgat gacctgcgcg atggcggtgc ccagcacttt
     1201 gcggattgca ttactgccat tcgggaaaaa agcccgcaaa tcaaaattga aactctggtg
     1261 ccggatttcc gcggtcgtat ggatcgtgct ctggatattc tgaccgccac accaccagat
     1321 gtgttcaacc ataacctgga aaacgtaccg cgtatttacc gtcaggtacg gcctggtgca
     1381 gattacaact ggtcgctgaa gctgctggaa cgctttaaag aagcgcatcc ggaaatcccg
     1441 accaagtctg gtctgatggt gggtctgggt gaaaccaatg aagaaattat tgaggtaatg
     1501 cgcgacctgc gccgtcatgg tgtgacgatg ctaacgctgg ggcaatattt gcagccaagc
     1561 cgccatcacc tgccggttca acgttacgtt agcccggatg agttcgacga aatgaaagcc
     1621 gaagcgctgg cgatgggctt tacccatgct gcatgcggtc cgtttgtccg ctcgtcttac
     1681 cacgccgatt tgcaggcgaa agggatggaa gttaagtaat cctgtaataa ttacttacat
     1741 ctgtctgata aaaaacccgc tttttgaagc gggttttttg tatcgaacaa gatattgagg
     1801 gagcgtcctg ctcgccacgt cactctttat gagagagctt ttcagcctga agatcaacgt
     1861 ctgcgccttt tttcgccgca gcatcgtcat cattcatcgc cttcttgaac cctttaatgg
     1921 ccgctccaag gtctccgccc agcgtacgta acttcttagt cccaaacagc agaacgacca
     1981 gcgccgcaac taccagcagt ttggtaatac taatctcacc catagatacc ttcttgacat
     2041 ataaccggca acagaacgcg ccttaaaaac cagagaatat taacggtcat tcgacgcgcg
     2101 cacacaatag caatctgtaa caaggtgaat caagcgccga gtaaaaaaac atcataataa
     2161 ttgcggcagc gcaaagcgac ggttgtttaa gacgggcagt tgcgcgcgta cctgacgcac
     2221 acgttcgggc gtcacttccg ccataatgag tgcaggcatt tctgacgctg ccgcaatggt
     2281 gacgccaaag ggatctataa tccggctttg cccgatattt ttgttcccgc actcccccgc
     2341 cgccaccata taacaggtgg tatccagcac acgagcggca agcaacgttg accagtgatg
     2401 ctctttgagc ggcccacgaa cccaggcggc aggaagtacc aggatttcag ctccctgtaa
     2461 tgcctgtgcc agcgccagct ctggaaagcg taagtcataa caggtcatca gaccgacctt
     2521 catcccttcc acctccagta acggagctat ttcattacca gcatcaacat ggcgtgattc
     2581 ctgaatggca aatgcatcat agagatgcag tttggcataa cgggcgacga tgtttcctgc
     2641 ctgtagcgcc accagcatat tccacgcgcg ccccggtgtt gaaggaacat gaatcgtcag
     2701 aattgtcgtc atcatgttac gtttactttc tcgccgtaaa cggtcgagga attcgccttc
     2761 cagcagctgt gctgatttaa ccgaaagatc cgcatcatga tcatcacgcg ccagtaatgc
     2821 ttccggcagg acaaacagcg aaacctcgtt ttccgccgcc tgcgccatca acgaggcaca
     2881 aatctcagcg ttcttttccc acacagatgt aacagcaaac tgtcctgcgg caaccagcat
     2941 tgccacctcc tgtcagagtc gtaaaattcg ccaacggcga ttattcggcg ttacacttat
     3001 cactcataca aatcaaatag caggattttg cagtgttaca acttctttta gcagttttta
     3061 ttggcggtgg tacgggaagc gtggcgagat ggctgttaag tatgcgattt aacccgctgc
     3121 atcaggcgat tccgttgggg acgctggctg caaacctgat tggggcattc atcataggaa
     3181 tgggattcgc cggttcagca ggatgacgaa cattgatcca gtgtggaaag tattaatcac
     3241 caccggattt tgtggcggtc taacaacctt ctcaacattt tcggcagaag tggtgttttt
     3301 gttacaagag ggccgctttg gctgggcgtt actgaacgtt ttcgtcaacc ttttagggtc
     3361 ttttgccatg accgcactgg ctttctggct gttttccgcc tcaaccgcac actaaagacc
     3421 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
     3481 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
     3541 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
     3601 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
     3661 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
     3721 ggttaccagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
     3781 aaagtcgcgt tatttttgat gttaattatt ttacccattg attttaaaga gtttactaaa
     3841 atttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttata
     3901 ggtacccgga atgtaggtca tctgaaaagt cactggatca taacccacag aggccaatgg
     3961 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
     4021 tcccagtcct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
     4081 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
     4141 ccagtttcct ttttcatccc aacgcgactg accaaaaccg ccaccccacg gtcgctcgtt
     4201 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
     4261 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgttgt
     4321 catccactca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
     4381 ggaaaagata
//
LOCUS       DAZGHF010000240         4352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGHF010000240 DAZGHF010000000
VERSION     DAZGHF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.241"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..192
                     /locus_tag="ACHK3A_004869"
     CDS             <1..192
                     /locus_tag="ACHK3A_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO8742818.1"
                     /translation="PEQVARQIPRLKAMLAMRSLLRDLKANLLDNATFRKELEKILRD
                     PELSQALRDELSALVPEQV"
     gene            213..1757
                     /gene="tssC"
                     /locus_tag="ACHK3A_004870"
     CDS             213..1757
                     /gene="tssC"
                     /locus_tag="ACHK3A_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO8742819.1"
                     /translation="MLMSVQKENNVAGNVVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKVLIDHHIAELDDQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALVSAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKETMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDT
                     VPVRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDL
                     PIHLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPA
                     LYDTPDATANSRINARLPYIFLLSRVAHYLKMIQRENIGTTKDRRLLELELNTWLRGL
                     VTEMTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAIPHFQVEGMDVSLSLVSQ
                     MPKAKA"
     gene            1773..3122
                     /gene="tssK"
                     /locus_tag="ACHK3A_004871"
     CDS             1773..3122
                     /gene="tssK"
                     /locus_tag="ACHK3A_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO8742820.1"
                     /translation="MKIFRPLWSEGVLLSPQQFQQQAEWESFRSAGISALASPFPWGV
                     EQVEFNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADRDAVTVV
                     IALPHMQPGVANVEQEGVSADRPVRYREEWVTLQDAFGSEEEPIAVARFNFTIRFAHE
                     NNDSWKVCPVARLIRDGQNAWRQDPSFIPPVALFGASPVLRERLVLLNRQLRSRRQRL
                     MAMRRESNERLADFAVADVSLFWLLNALNSHASVLTEYERFPSRPPEQVWAELARLAG
                     SMLTFSLDHDPDAIPGYDHKDPAGAFLPLFDLITGLLEACLPSRVIALEMSRPDGQTW
                     KASLHDIRLREEADLWLSVRSDTPAWQVAEKFPALCLAGSPDNVSEIYGAALRGIPLI
                     PVSRVPAALPVRMENQYFALDMESPAAHEMLEQGVCMFYVPELLGALELELFAVLRS"
     gene            3119..3808
                     /gene="tssL"
                     /locus_tag="ACHK3A_004872"
     CDS             3119..3808
                     /gene="tssL"
                     /locus_tag="ACHK3A_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO8742821.1"
                     /translation="MRQDIDIDQLMMETWLTVTMLKKGALTPDGTALYNKCVKQVESV
                     REALERAGYDDASVEHITYAQCALLDETVMSRKPEKSGEGGEPDVDEGQAAWRKVPLQ
                     ARFFGSLRAGEALWDRIAEVLRQPSPDPAVLTCFHRVTALGFQGVYGLKAVSQSQRDE
                     VMKALSERVSLPDGGLSLVVYRMEKHRWRLIRSVWFWTVLAVILTGVIWWGGYLWLQS
                     LLSSQIPELHG"
     gene            3808..>4352
                     /locus_tag="ACHK3A_004873"
     CDS             3808..>4352
                     /locus_tag="ACHK3A_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8742822.1"
                     /translation="MRGSVIIPVALHALVAVSTLLWLLWYFIPAGNVFNGLATLLILL
                     LAGWSVFKNSRSEKQKLTSDVTLTDAEPALSDTRGPVVLVCGDMPEALFQDGPLRKTA
                     RGWWLRVGDVSRLTDVVRSIQTQFPRQVGQLSVMYRCLPDCHHDEAVLRFTLKTLRQQ
                     CKQIKSLTGFTLPVVLSAEFSG"
BASE COUNT          908 a         1068 c         1286 g         1090 t
ORIGIN      
        1 ccggagcagg ttgcccgaca aattccccgt cttaaagcca tgctggccat gcgaagtttg
       61 ctgcgggatc tgaaggcgaa tctgctggat aacgccactt tccgtaaaga actggaaaaa
      121 attctgcggg atcccgaact gagtcaggcc ttacgtgacg aactgagtgc actggtgccg
      181 gaacaggtgt gattaacgga ttaagagaga gcatgctgat gtctgtacag aaagaaaata
      241 atgtggcggg aaatgtggtt tctgaaacgc attccggcga cagcgtatat gcttccctgt
      301 ttgaaaaaat taatctgaat ccggtatcag ccctgagtgc gctggatatc tggcaggatc
      361 cgcaggccat gtcagaggca tcagcagatg aacgtctgac cgcagggatg caggtgttta
      421 tggaatgcct ggcgaaagcg ggagcaccgg tggagaaact ggataaggtg ctgattgacc
      481 atcatattgc agagcttgat gaccagatca gtcgtcagct ggatgccgtg cttcatcatc
      541 cggaatttca gaaagtggaa tccctgtggc ggggcgtgaa gtctctggtg gataaaacgg
      601 acttccgccg taatgtgaaa attgagctgc tggatctgtc caaagacgat ctgcgccagg
      661 attttgagga tgcgccggaa attattcaga gcggccttta tctgcagacc tatgtggctg
      721 aatatgacac gccgggtggt gaacccattg ctgccctggt ttcggcctgg gaatttgatg
      781 catctgcgca ggatgtggcg ttgctgaaaa atatttcccg ggtggcagca tccgcacata
      841 tgccgtttat tggttctgtt ggtccggctt tcttccggaa agaaaccatg gaagaggtgg
      901 ctgctattaa ggatattggc aatcactttg agcgtgccga gtacatcaag tggaacgctt
      961 tccgagagac ggatgatgcc cgctatattg gtctggtgat gccccgtgtg ctgggacgcc
     1021 tgccgtatgg ccctgatact gtacctgtcc gcagctttaa ctatgttgaa gaggtgaaag
     1081 ggccggatca tcacaagtat ctctggacga acgcgtcttt tgcttttgcg gccaatatgg
     1141 tgcgcagttt tgtcaccaac ggctggtgtg ttcagatccg cggtcctcag gccggtggtg
     1201 cggtgcagga tttgccgatc catctgtatg atctgggaac cggtaatcag gtcaaaattc
     1261 cgtcagaggt gatgatcccg gaaacgcggg agtttgaatt cgctaatctg ggttttattc
     1321 cgctctctta ctacaagaac cgggattatg cctgtttctt ctccgctaac tccacccaga
     1381 aaccggcact gtacgatacg ccggatgcaa cggcgaacag ccgtattaat gcccgtcttc
     1441 cctatatttt cctgctgtcc cgcgttgcgc attacctgaa gatgattcag cgggaaaata
     1501 tcggtaccac caaagatcgg cgtctgctgg agctggagct gaatacctgg ctccggggcc
     1561 tggtgacgga gatgaccgat ccgggcgatg agcttcaggc atcgcatccg ctgcgtgacg
     1621 gaaaagtggt ggtggaagat attgaagata acccggggtt cttccgtgtg aaattgtatg
     1681 ccattccgca tttccaggtg gaaggaatgg acgtcagcct gtccctggtt tcgcagatgc
     1741 cgaaggcaaa agcgtaaggc aggtgtcggg aaatgaagat tttccgtccg ctgtggagcg
     1801 aaggtgtttt gctctcccct cagcagtttc agcagcaggc tgaatgggag tcttttcgca
     1861 gtgccggtat ctctgcgctg gcatccccgt ttccctgggg agtggaacag gttgagttta
     1921 atgacagtct tctgtcgtcc gggctgatcc agattacgca gttacgcctc tggctggaag
     1981 acggaacact tattgatgct caaaggagcg atctgcctcc ggcgccacgt gagttagatg
     2041 ccggtcagct ggctgaccgc gatgcggtaa ctgtcgttat tgcacttccc cacatgcagc
     2101 caggcgttgc caacgttgag caggagggtg tcagtgcaga cagacccgtt cgctatcggg
     2161 aagagtgggt cacgctgcag gatgcttttg gctctgaaga agagcccata gccgttgccc
     2221 ggtttaattt caccatccgg tttgcccatg aaaacaatga ttcatggaaa gtctgtccgg
     2281 tagcaagatt gatcagagat ggtcagaatg cgtggcgaca ggatccgtct tttattcctc
     2341 cggtggcgtt atttggggca agtccggttc tgcgcgaacg tctggtgttg ctcaatcgtc
     2401 agttacgctc ccgacgccag cgtctgatgg cgatgcgtcg tgaaagtaac gaaagactcg
     2461 ccgattttgc tgtcgctgat gtttcccttt tctggttgct gaatgcgctg aactcccacg
     2521 ccagtgtact gacggaatac gaacgttttc cttcccgtcc acctgaacag gtgtgggcgg
     2581 agctcgctcg tctggcaggc agtatgctga ctttttccct ggatcatgat cctgacgcca
     2641 taccgggtta tgaccacaag gatccggccg gtgcgtttct tccgctgttc gacctgataa
     2701 cgggtctgct ggaggcctgt ctgccatccc gggtcattgc tctggaaatg tcccgcccgg
     2761 atggacagac ctggaaggcc agccttcacg atatccgcct gcgcgaagag gcggacctct
     2821 ggctgtctgt gcgctcggat actccggcct ggcaggttgc tgagaaattt ccggcactct
     2881 gtctggcggg ttcacctgat aatgtcagtg agatctatgg cgcggcactc agggggatcc
     2941 ctttgatccc ggtgagcagg gttccggctg cgttgcctgt acgtatggaa aaccagtact
     3001 tcgcacttga tatggaaagt cccgctgcac atgaaatgct ggagcaggga gtgtgtatgt
     3061 tctatgtgcc tgagttactt ggtgccctgg aacttgaact gtttgcggta ttgcgctcat
     3121 gagacaggat attgatatcg accagctgat gatggagaca tggcttaccg tcaccatgct
     3181 gaaaaaaggt gcactcacgc ccgatggcac tgcgctttac aacaaatgcg ttaaacaggt
     3241 cgaaagtgtg cgggaagcgc tggagcgtgc cggttatgac gatgccagtg tggagcatat
     3301 tacctatgca caatgtgccc tcctggacga aacggtgatg agccgtaagc ctgaaaaaag
     3361 cggggaaggc ggggagccgg acgttgacga aggccaggcg gcctggcgta aagtgccgct
     3421 tcaggcccgc ttttttggtt ctcttcgggc tggtgaagca ctgtgggatc gcattgcaga
     3481 ggttctgcgt cagccttcac cggacccggc cgttttaacc tgctttcacc gggtgacagc
     3541 actgggtttt cagggggtgt acggcctgaa agcagtcagc cagtcacagc gtgatgaggt
     3601 catgaaagcc ctttctgaac gtgtttctct gcctgatggt ggtctgtccc tggttgttta
     3661 ccggatggag aaacatcgct ggcgtctgat acgctcagtc tggttctgga cagtgctcgc
     3721 cgtcattctt accggcgtta tctggtgggg ggggtacctc tggcttcagt ctcttttgtc
     3781 atcacaaata ccggagttgc atggctgatg cgtggatctg tcatcattcc ggtagccctg
     3841 catgccctgg tggccgtttc cacgttgctg tggttgctgt ggtatttcat cccggcaggc
     3901 aacgttttta acggtctggc tactctgctg atccttcttc tggcaggctg gagtgttttt
     3961 aaaaacagcc ggagtgaaaa gcaaaagctc acatctgacg ttaccctgac ggatgctgaa
     4021 ccggcattat ccgacacccg gggccctgtt gtactggtat gtggagacat gccggaagcg
     4081 ttgtttcagg acggtccgct gcgtaaaaca gcccggggat ggtggctgcg ggtcggtgac
     4141 gtcagtcgct taactgacgt tgtccgcagc atacagacgc agttcccgcg ccaggtcggt
     4201 cagctctctg tgatgtaccg ctgcctgcca gactgtcatc atgatgaagc ggttcttcgt
     4261 tttacactga aaacactccg ccagcagtgt aagcagataa aatccctgac gggtttcaca
     4321 ctgccggtgg tgttgagtgc cgaattttcc gg
//
LOCUS       DAZGHF010000241         4269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGHF010000241 DAZGHF010000000
VERSION     DAZGHF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.242"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(19..222)
                     /gene="ydfZ"
                     /locus_tag="ACHK3A_004874"
     CDS             complement(19..222)
                     /gene="ydfZ"
                     /locus_tag="ACHK3A_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO8742823.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(399..1085)
                     /gene="rspR"
                     /locus_tag="ACHK3A_004875"
                     /gene_synonym="ydfH"
     CDS             complement(399..1085)
                     /gene="rspR"
                     /locus_tag="ACHK3A_004875"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO8742824.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTHHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(1174..1920)
                     /gene="ydfG"
                     /locus_tag="ACHK3A_004876"
     CDS             complement(1174..1920)
                     /gene="ydfG"
                     /locus_tag="ACHK3A_004876"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO8742825.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDSKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            2057..4102
                     /gene="dcp"
                     /locus_tag="ACHK3A_004877"
     CDS             2057..4102
                     /gene="dcp"
                     /locus_tag="ACHK3A_004877"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO8742826.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQAPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELVSIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KSAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            complement(4147..>4269)
                     /locus_tag="ACHK3A_004878"
     CDS             complement(4147..>4269)
                     /locus_tag="ACHK3A_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742827.1"
                     /translation="KFENYYNGRVYKKSNFAGDCETAFALAVKFALTQRLQLLL"
BASE COUNT         1047 a         1047 c         1082 g         1093 t
ORIGIN      
        1 tcggcggcat tcacacgctt agttcatgcc gagacgaatc aggtccagag gtgccagttt
       61 ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg atttgctcag cggtcagacc
      121 ttcagtatca atcgacagga tcttgccagt gtgaccggtg ccgctaacca taacacggct
      181 gccagtggtg attgcgttac ggttacgatc gtaggtcgtc ataggtgttt tctcctttct
      241 gatttacagt cgaagcccac gtaacgggcg cactataaat actcctgtcg agtgaaatat
      301 tttttgtttt tgatcaagct cacatctttt ttgatgcatg catgagccat atgacggagg
      361 gaaaaactct caccccggat gggatgagag gaaatgaatt actcttcgct aaaccagtcg
      421 ctgttttcct gacggatctg gcgcacggat tcgctgattt cctgcaaatg gtgcgtcatt
      481 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatctc aagatgctgg
      541 cgtaacagca tttctggtgg agaaacgtgg tcgaaactca tatagcgcac gcgatcaacg
      601 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
      661 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctctatggca
      721 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
      781 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
      841 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
      901 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
      961 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     1021 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     1081 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     1141 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     1201 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     1261 tgacacccac cagacggctt cgctgacatc ttccggcgtc aatgcaacgg tattttgata
     1321 ggttttttcc gctttactgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     1381 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcaggt ccgtacgcag
     1441 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     1501 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     1561 acccggtaag acggcgcgcg tcatatatac caggcctttg ttgttggtat caatcatcgt
     1621 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     1681 atttaccagg atatcaatat tgcaccactc ggcaggaagc gatgccagca tctcttcaat
     1741 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtcttt
     1801 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     1861 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     1921 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     1981 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     2041 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     2101 tctggctccc cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     2161 aatgcagcaa aagcgggcag aaattgctgc catcgcgctt aacccgcaag cgcctgattt
     2221 caacaatact attctggcac tggaacaaag cggagaatta cttacccgcg ttaccagcgt
     2281 cttttttgcg atgactgcgg cgcatactaa tgatgaatta cagcgtcttg atgaacagtt
     2341 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     2401 ggtagatgct gtctggcaac gccgtgaatc cctgggactt gatagtgaat ccatccgcct
     2461 ggtggaggtc attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     2521 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     2581 gttactggca gcaaataaat ccggcggtct ggttgtgaac gatttcgcgc agctggcagg
     2641 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     2701 caaatggctg attccgttgc tgaataccac ccagcaacca gcgcttgctg aactgcgcga
     2761 tcgcgcgacg cgtgaaaaac tgttcactgc gggctggacg cgagcggaaa aaaatgatgc
     2821 caatgatacc cgcgctatca ttcaacgtct ggtggagatc cgcgcgcagc aggcgaaact
     2881 gcttggtttc cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     2941 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     3001 attagtctcc atacaggcgg ttattgataa gcagcagggc gggtttagcg cgcagccgtg
     3061 ggactgggca ttttatgccg agcaggtaag acgtgagaaa ttcgatctcg atgaatcgca
     3121 gctcaagcca tattttgaat taaacacggt gttgaatgaa ggtgtattct ggaccgcgaa
     3181 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt cctgtctacc atcctgacgt
     3241 tcgtgtgtgg gaaatttttg atcataatgg cgtggggctg gcgttatttt acggtgattt
     3301 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     3361 gctgaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaat ccgccgctgg
     3421 ggagcctgcg ttgttactct gggatgatgt cataacctta ttccatgaat ttggtcatac
     3481 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     3541 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     3601 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgatgaac tgcaacagaa
     3661 aatgcgtaat gccagcctgt tcaataaagg gtatgagatg agcgaactgc ttagcgccgc
     3721 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     3781 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     3841 tcgcagcagt tatttcgccc atatttttgg tggcggatat gccgcaggtt attacgctta
     3901 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     3961 aacgcgtgaa aatgggcggc gttttcgcga ggcgattctt tccagaggta acagcgaaga
     4021 tctggaacgc ctgtatcgac aatggcgcgg taaggcacct cagattatgc cgatgctgca
     4081 acatcgtggc ttgaacgtat aaacaccacc aatggctggc ctggatttat cgccagacca
     4141 gcctcgttat agcagtaatt gcagccgctg ggtgagggca aacttcaccg ctaatgcgaa
     4201 tgccgtttcg cagtcgccag caaaattact ttttttgtat acccttccgt tataataatt
     4261 ttcaaattt
//
LOCUS       DAZGHF010000242         4255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGHF010000242 DAZGHF010000000
VERSION     DAZGHF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.244"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1188
                     /gene="ybaT"
                     /locus_tag="ACHK3A_004879"
     CDS             <1..1188
                     /gene="ybaT"
                     /locus_tag="ACHK3A_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO8742828.1"
                     /translation="GQAALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIID
                     FFRRGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALG
                     IIAVMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAF
                     FSCIGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVS
                     ALELEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSER
                     ELPKLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIR
                     LRHDIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRN
                     KTV"
     gene            1219..1316
                     /locus_tag="ACHK3A_004880"
                     /pseudo
     CDS             1219..1316
                     /locus_tag="ACHK3A_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1313..1720
                     /gene="cueR"
                     /locus_tag="ACHK3A_004881"
     CDS             1313..1720
                     /gene="cueR"
                     /locus_tag="ACHK3A_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO8742829.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPDDDSADCPIIENLSGCCHHRAG"
     gene            complement(1721..2451)
                     /locus_tag="ACHK3A_004882"
                     /pseudo
     CDS             complement(1721..2451)
                     /locus_tag="ACHK3A_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2523..2729)
                     /locus_tag="ACHK3A_004883"
     CDS             complement(2523..2729)
                     /locus_tag="ACHK3A_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742830.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(2726..3148)
                     /locus_tag="ACHK3A_004884"
     CDS             complement(2726..3148)
                     /locus_tag="ACHK3A_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742831.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNADATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(3234..4250)
                     /locus_tag="ACHK3A_004885"
     CDS             complement(3234..4250)
                     /locus_tag="ACHK3A_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO8742832.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     TQTPARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLSNTQKRVADNSQQINTLNNHYSSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
BASE COUNT          946 a         1027 c         1116 g         1166 t
ORIGIN      
        1 gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt tggcggtatt
       61 gtggcgatgt tttccggtta tgcctatgcg cgcctggggg cgagctatcc cagcaatggc
      121 ggtattatcg acttctttcg tcgcggatta ggcaacggcg tcttttcgct ggcactctcg
      181 ttattgtacc tgttgacgct ggcggtgagc atcgccatgg tcgcccgtgc ttttggcgct
      241 tatgccgtgc agtttttgca tgaaggcagt caggaggagc accttatatt gctctacgcg
      301 ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc ggtagggcgg
      361 ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat cattgccggt
      421 gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc cggtgcgttc
      481 ttctcctgta tcgggataac cttccttgcc tatgcgggct ttggcatgat ggcgaacgcg
      541 gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt ggcgattggc
      601 gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt atcggcatta
      661 gagttagaaa aatatgccga taccgccgta gcgcaggctg cttctccgct gctcgggcat
      721 gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc cataaacgcg
      781 aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga actgccgaag
      841 ctaatgaata aatccctgtg gcggcagagt acctggggca acatcattgt cgtggtgctg
      901 attatgctga tgacggcggc gctgaattta ggctcactcg ccagcgttgc cagcgccacc
      961 tttttgatct gctatctggc ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac
     1021 gcctcgttgc cgattcttat cgttggtacg ttggttatgt tgttggtgat cgttggcttt
     1081 atctacagtc tctggtccca gggcagccgt gcgctgatat ggattattgg cgcactctta
     1141 ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ctgtataaca tctctctgtg
     1201 cgcagtacct cctgtattat tgtggtggcg gtcgatattc gcactggcaa aaaaacgtgc
     1261 ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat
     1321 tagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct atgaagagaa
     1381 ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca cgcagcagca
     1441 tctcaacgaa ctgaccttac tgcgccaggc acgccaggtg ggctttaacc tggaggagag
     1501 cggcgaactg gtgaatctgt ttaacgatcc gcagcgtcac agcgccgacg tcaaacgtcg
     1561 tacgctggag aaagtggcgg agatcgaaca gcatatcgaa gaactgcaat ccatgcgtaa
     1621 ccagctgctg gcgctggcga atgcctgccc cgatgatgac agcgccgact gcccgattat
     1681 cgaaaatctc tccggctgct gtcatcatcg ggcggggtga ttaaggcttg cgaaaatagt
     1741 cgtccattat tttcgtcagc tccggacgtt ccccgtcatc attgatggca aaatccagaa
     1801 aaaaagcatc tgcttgcggc gttcggtttt gcgccatcaa ccggaaggtg agggggcctg
     1861 accaggtttc ggatgggctg atgtgcttat cagggccgaa actctcccat gcgttgggcg
     1921 gtaatgtttt gcacgcggtg agttttggta tgtggtattg cagctctgtt ttgtgcagac
     1981 attccccgcc aggaaatacc agggtttgtt gctcatcatt aaaatgggtt gccgtgctga
     2041 gcaccagtgt tttgagcggt cgcccttcca gcgcggcggc agtagcctta tggtgtccgt
     2101 cgagcagaac gcagagatag gaatcaggga gataccaggc cagagcgcgg gcgattccag
     2161 ttttcgagag cagacaaaaa ccgggcggct ccctgttcac cctccgcaaa accgctgtag
     2221 acaaattccg cacaggtagg gcaaccagcc tcggaccaga aacgtattaa cgggacatcc
     2281 ttgccagcag agcgaatgga taagaccgtg cgagtgcgga tcgtaaatca cacattgacg
     2341 cagaaatcct ttcccggact tcaccgaaaa aatgacgccc gcagcggtat ttttcagcag
     2401 acgtacttca ggttttcgaa aggggaaaaa gcatggccac tccttagcca aaatttcagt
     2461 tccctgaact gtgccagaaa attacgctgg tgactattgc tctggaaatg gaaacagtgt
     2521 ccttacccat taatccccgt ttgtaccgaa agaatatgtt gcaaactttc taccataatg
     2581 cgccccgact gaaataagcc ttcctggatt tcattgttaa cattcacagc gtgaaccagg
     2641 gaggctaata acaccacttg caagaaggca aaaataaacg caccgataat cataatatgc
     2701 gtcaggggga ttttcctttg ctccttcatt ttaaataacc cacaaactgg ccttctttca
     2761 ttaaggcgac ttttcctgtg tgatgataaa caatgacatt atacagaccc gactgataaa
     2821 ccgtggcatc ggcattatct tcatctatgg cacataatga atcattgaga taagcaaaat
     2881 tcttactact cattctaaag gtcatgccaa atcctttata aatggctttt tttgccgcct
     2941 gggcatgttg tgcggcttct gcatttttaa atgcctgcgc ttcagaagca cttaaaatag
     3001 ccgtctggcg ctgctgttct gcgagataac aggtgtcatg gctgacgccc tgcgcctgac
     3061 aatctgcgat gcgggaagcg ggtgagctac agccagccag taatataacc gccagcaggg
     3121 aaaaggattt aatggagcta ttcatcattg ttaaccttaa ataatgtgcg aattaatgaa
     3181 gaggtgaaac ccggcaagct tccttgctct tatttatatt gaggattgaa atattagaac
     3241 gaatatccta cgccgacacc tgcgccgaaa tcagattgcg tatccgcaga aataccggct
     3301 ttaagtaccg tatgcgttcc ggcattaaaa gatgttccga cagacaccgc agattcaccg
     3361 ttgaaggttc ccgctcccgc tgacaccatc atcacgtcac cggttttaac ctgtggttgt
     3421 gaggcgatag cgatggcaga tgcagttccc gcattggctt ctttacggtt gtcctcaact
     3481 tcgtttttca gcgaactgta atggttattg agcgtgttaa tttgttggct gttatctgcg
     3541 acccgttttt gcgtgttgct taactgttgc tgcgtttgtg cggaatcttg tctcagactt
     3601 tcaatttgct catgtgcctg gacagacttc tgcgccagac tttgcatttg tttatcgtat
     3661 tgagcccttt ctttttgctg ttgtgctgcc acatcggttg tcagtgcatt cattttctgc
     3721 ccttgttccg tggtagtgcg ctgattagcc tcattttgag ccgccagtct ggcattaatc
     3781 atctcctgat aatgcgcagt attttgcgta tctccagtcg cggcgagcgt tttttgtgtg
     3841 gctacgatct gcgcctgttg ctctgtaatt tgcgtttgct gggcatcagt taccgcagac
     3901 tgtcgggcgt tgatcatgct ctgataacgg gcaggtgtct gtgtatcacc tgtagcggca
     3961 acggtttttt gtcctgcgtc aggatctgtc tggacagtgg gagtctgagt ggtatcaggt
     4021 gtttcagaag ggatgtagtg atgttcagtc aaataggtat acgcagcggg gctgttgtct
     4081 gtgcgttcta aaaagcgggt aatcttctgc tcaatctccg attgcgacat gccagagtta
     4141 atggctgaaa gctctgttgc cgccgcttca atggtatcac cagctaaaac aacagggctg
     4201 gatgttgctg aaattatgag tgccagtaaa gctacgttta cagttttcat tcatt
//
LOCUS       DAZGHF010000243         4248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGHF010000243 DAZGHF010000000
VERSION     DAZGHF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.245"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(42..2636)
                     /locus_tag="ACHK3A_004886"
     CDS             complement(42..2636)
                     /locus_tag="ACHK3A_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO8742833.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDENQLIPCLSLAD
                     LLSLGINKNALPEQALALSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHSKYSTTVGQYRSNSYNQKSPYVFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKTLDDKDYVYNWNDFYNLRYS
                     KRGKFQASVSQPFGNYGSMYLSASQQTYWNTDKKDSLYQVGYNTSIKGIYLNVAWNYS
                     KSPGTNADKIVSLNVSLPISNWLSSTNDGRSSSNAMTATYGYSQDNHGQVNQYTGVSG
                     SLLEQHNLSYNIQHGFANQDNSSSGSVGVNYRGAYGSLNSAYSYDNEGNQQINYGISG
                     ALVVHENGLTLSQPLGETNVLIKAPGANNVDVQRGTGISTDWRGYAVVPYATEYRRNN
                     ISLDPMSMNMHTELDITSTEVIPGKGALVRAEFAAHIGIRGLFTVRYRNKSVPFGATA
                     SAQIKNSSQITGIVGDNGQLYLSGLPLEGVINIQWGDGVQQKCQANYKLPETELNNPV
                     SYATLECR"
     gene            complement(2673..3425)
                     /locus_tag="ACHK3A_004887"
     CDS             complement(2673..3425)
                     /locus_tag="ACHK3A_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8742834.1"
                     /translation="MKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAG
                     GFGLGVTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFVITPPLFML
                     PTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLALQNKIKLFY
                     RPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNTFVPPKSDI
                     TVSSAVTLAGEITYQTINDFGATTERQKGIMQ"
     gene            complement(3526..4230)
                     /locus_tag="ACHK3A_004888"
     CDS             complement(3526..4230)
                     /locus_tag="ACHK3A_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8742835.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDAAQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDIEAQ
                     GASNQTDFNINLTECSNTEDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT         1220 a          962 c          811 g         1255 t
ORIGIN      
        1 aagaataaaa aatacagaat atttagagaa tattaaattc attagcggca ctccagagta
       61 gcatagctaa caggattatt cagttctgtt tcagggagct tgtaattagc ctgacatttt
      121 tgctgaacac catctcccca ctggatatta ataacacctt ctaaaggcaa tcctgagaga
      181 taaagttgtc cattatcgcc gacaatcccg gtaatttgac tactattttt aatctgtgcg
      241 ctggctgtag caccgaatgg gactgattta ttacgataac gaactgtgaa caaaccacga
      301 ataccgatat gagcagcaaa ctctgcacga actaacgcac cttttcccgg aataacttca
      361 gtggaagtga tatccagttc agtatgcata ttcattgaca taggatctaa tgaaatatta
      421 ttacgtctat attctgttgc ataaggaaca actgcatatc cacgccagtc agtggatatt
      481 cctgtccccc gctggacatc cacattattc gctccaggcg cttttatcaa aacattagtt
      541 tcacctaatg gttgactcaa cgtaagacca ttttcatgta caacaagagc accactgatg
      601 ccatagttta tctgttgatt accttcatta tcgtaactgt aggcggaatt caaggaacca
      661 tatgccccac gataattaac accaacagaa ccactactgc tattatcctg attagcaaaa
      721 ccatgttgta tgttataact gagattatgc tgctccaaca gagaaccaga tacccccgta
      781 tattggttta cctgtccgtg gttatcctga ctataaccat acgttgcagt catggcattc
      841 gatgatgagc gcccatcatt cgtggaagat aaccaattac ttataggtaa tgagacattt
      901 agcgagacaa ttttatccgc atttgtccct ggtgatttac tgtaattcca cgcaacattt
      961 agatagatac ccttaatact ggtgttataa ccaacttggt ataaagaatc ttttttatca
     1021 gtattccagt atgtttgctg actagccgat aaatacatag acccgtagtt accgaaaggt
     1081 tgcgatacac tagcctgaaa ttttccacgt ttgctataac gcaagttata aaaatcattc
     1141 cagttataaa cgtaatcttt atcatctaac gtttttggat caacaacagt ccctgacatt
     1201 tgttggtatg tcgtatcact taaagtgtaa aagccctggg ttgaataacg atagccaata
     1261 atatggaatg ctgttcctgt ctgaactaac gatttggaat aaagaaaacg ataagattgc
     1321 ccttgatgtt tgctcccatc cacaagcgaa ctgttagcct gagtaacatc aaacgatgtt
     1381 gcaccaaata cacccagatt caggccaagg ccgagcgcca acgcccggta atcctcagag
     1441 aattgtgccc caccataagc cgtaatatcc cagggtaaac cccaaattaa ttccccctga
     1501 aatacataag gacttttctg gttatagcta ttgcttcgat attggccaac cgtagtagaa
     1561 tattttgaat gaccttctcg ttgcaggatg gggacagcgg cataaggtac ggtatagaca
     1621 gtttctgaat tatcagactc tttgatggta acttccagat ctcccgcaga tgatgtcggg
     1681 ttaagatcgc taatctcaaa cggtcccggc ggcatatagg tttgataaat gacataacca
     1741 ttctgtttga ttgttacctg agcgcgactt ttcgcaattc cgcgcaccgt gggagcgaaa
     1801 ccactttgac tatcgggcaa catattttcg tcagattcca gttttatgcc acgaatacta
     1861 acactgtcga aaacatctga tgatgaccat gtatctccta gcgttaattc acttctgaaa
     1921 ggaataataa cgcgctgcaa cgtactactg atatgctcca gtttacctgc tgaattactc
     1981 ccacgactcc aggttgaata atcgcgaaaa cgccatgcac caatattgat cccagcgcga
     2041 agatttaaat aatagttatt tgaagagtaa tcaccacgat cgttattacc agaaaagtca
     2101 taattcagca aaaaagctgt aataccgtta tcccactgtt ctggtggaat atatccgcgg
     2161 gcgtcacgtt ttattatcgc ctgcggaaag gtcagtttaa gtctctgtgc atccagctcc
     2221 ggcatgtaat gcacatcagg aaaccagata cgcaaatcaa ggcaactatt ttcagataaa
     2281 gccaaagcct gctctggcag cgcattcttg ttaattccaa ggcttaataa gtcagcaagt
     2341 gaaaggcagg gaatcagttg attctcatcg tctgctttaa aagttacagt acgagagagt
     2401 aaaggggaat cattgaccca aacttcgact tcatagctac caggaagata agtcatccct
     2461 ttctcgaaac gggataagtc agcaacagct tcagtatcac ccgacagaaa agcagggtta
     2521 aagcgcattc ccgcgattga acatgcaggc ataataacca gaagaagcgg tacaacccgt
     2581 ttcatctgct tttgtgccgc catccaatat tcgcatcttt tgagctttag gttcatatcc
     2641 ctgacctgtg ttgcatctga actatcccgt tgttactgca ttattccttt ctgccgctcc
     2701 gtcgtagcgc caaagtcatt aattgtttga taagtaattt caccagccaa cgtcactgcc
     2761 gacgagaccg taatatcgct tttcggtggt acaaacgtat tgggcaattt actcccttgt
     2821 actttaattc ctgtcagtgt caggtaataa ggcgaaggat tgataacttt tagttgtttg
     2881 ccctcatact taaaccgcaa catatttcgt gcttttcctg gttgcacagg tagattttcc
     2941 gggcgataaa atagcttgat tttgttttgt aatgccaact gtaaggtatt cttctgtgta
     3001 tttttctcat ctgttggcgg aattgcttta acattcatcc aatacaaagt ttcacgatcg
     3061 gtcggcaaat tagttttacc aagaaacatg atacgtaatg aagcttcttt acgcgttggc
     3121 aacataaaga ggggaggagt gataacaaaa tcctggctct ttttttccgt tttactgtca
     3181 tcaacccatg attgaattaa ataactggtg ttgtcaccgc tattttgcac actgagtgtt
     3241 atttgcttac tttctgccgg ataaatcaat cgcgttacgc caaggccaaa cccgcccgcg
     3301 gacgcattat taataaataa tatcgatagt aaaaaaagaa tccctgagcg tataatattt
     3361 tgtgatactt ttaattcatc cctgaatcca taaacccatt ttttatttcc aataatacat
     3421 ttcatacagc atccttgttg atgaaataac acctgttatt catactctat taaataattt
     3481 caatctcagg ttacaacttt ttgtaacctg aaatcgtatt tatatttact cgtacagcac
     3541 cacaaaactt gctgttgact taactacacc aggtgcaaca gtgctaccgt tttgagcata
     3601 acctgcagta aaggccgcgc tgaaatcatg ggtcgtagca tttcctggaa ttgtataagc
     3661 ggcggtggag gcgctaccct ctgccgcagt agtattcagg ttaatcagac cattattcgt
     3721 cgtacctttg gagtaaattc gaatccctac accagtcgcc cccgcattgc cgctgttgtc
     3781 agcatccgtt gatccttcta cttcgttagc gagcgtttta ttagcgctat ctgcggtacc
     3841 ggtaaagcgg aactcaattt tctgatcctc tgtattgcta cattcggtca gattaatatt
     3901 gaaatcggtc tggttgctcg ccccctgtgc ttctatgtcg ataccagtga aaaggttgga
     3961 aggcacaacc ggcaggacaa cttcaatctg ttttgaatcg gttgcgacct tacaggtagc
     4021 tttcactaca cggccagtaa agcggatttt cccccccatt gaatcaacgt tgtcatccat
     4081 atcagtgagc ctgggataat caacggtgct ccagtcagta tccgctccac caaattgtgc
     4141 tgcatcgccg ggtgctgcgg ttaccgcagt agacatcagc atacctgatg ccagtaatac
     4201 tgcggataaa ttagataaat tgaatttcat ttataccttt ccttttgt
//
LOCUS       DAZGHF010000244         4244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGHF010000244 DAZGHF010000000
VERSION     DAZGHF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.246"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            83..1741
                     /gene="dpiB"
                     /locus_tag="ACHK3A_004889"
     CDS             83..1741
                     /gene="dpiB"
                     /locus_tag="ACHK3A_004889"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO8742836.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIHSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1710..2390
                     /gene="dpiA"
                     /locus_tag="ACHK3A_004890"
                     /gene_synonym="citB"
     CDS             1710..2390
                     /gene="dpiA"
                     /locus_tag="ACHK3A_004890"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO8742837.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2431..3816)
                     /gene="dcuC"
                     /locus_tag="ACHK3A_004891"
     CDS             complement(2431..3816)
                     /gene="dcuC"
                     /locus_tag="ACHK3A_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO8742838.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1169 a          977 c         1069 g         1029 t
ORIGIN      
        1 ttatatttaa tatctatgta ttttgattca acattttaat tacattcgtc aaagaggctc
       61 gggacaaccc gcaaggaaaa caatgttgca gcttaacgag aataaacagt ttgcattttt
      121 ccaaagactg gcatttccgc tgcgtatctt tttgctgatt ctggtgttct caatatttgt
      181 cattgcagcc ctggcgcaat attttacggc cagttttgag gactatttaa cgcttcatgt
      241 gcgcgatatg gcaatgaatc aggcaaaaat tattgcttct aatgacagta tcatcagcgc
      301 agtgaaaacg cgtgactaca agaggctggc gaccatcgct gataaattac aaagagatac
      361 tgatttcgat tatgtggtga ttggcgatcg gcactcgatt cgtctttacc atcctaatcc
      421 ggagaaaatt ggttatccta tgcagttcac caaaccgggc gcgctggaga aaggggagag
      481 ctacttcatc accgggaaag ggtcaattgg catggcgatg cgtgccaaaa cgccaatctt
      541 tgatgacgat ggaaaagtca tcggcgtggt gtcgattggc tacctggtga gtaaaatcga
      601 tagctggcgg gctgagtttt tattaccgat ggctggcgtg tttgtcgtgc tgttagggat
      661 tctgatgttg ctgtcgtggt tcctggccgc gcatatccgt cggcagatga tgggcatgga
      721 gccaaagcaa atcgcacgcg tggtccgtca gcaagaggcg ctgtttagtt cggtttatga
      781 agggctcatt gcggtggatc cgcacggtta cattaccgcc atcaatcgta acgcaagaaa
      841 gatgctgggg ctgagctccc ccggacggca atggttgggt aaacccattg ctgaagtggt
      901 caggcccgcc gatttcttta ccgaacagat tgatgaaaaa cgtcaggatg tggtggcgaa
      961 ctttaacggt ctgagcgtta ttgccaaccg ggaagctatt cattctggtg atgatttgct
     1021 gggggccatt atcagctttc gtagtaaaga cgaaatatcc accctcaatg cacaactgac
     1081 gcaaattaaa caatacgtcg agagcctgcg cacattgcga cacgagcatc tcaattggat
     1141 gtcgacgctc aatggtctgt tgcagatgaa agagtatgat cgcgtgttgg cgatggtgca
     1201 gggggagtct caggcccagc aacagcttat tgatagcctg cgcgaggcgt ttgccgatcg
     1261 ccaggtggcg gggctgcttt ttggtaaagt gcagcgcgcc cgcgaactgg ggctaaaaat
     1321 ggtcatcgtc cccggaagtc agctttcgca actgccgcca ggactggaca gcaccgagtt
     1381 tgcagccatt gttggcaatt tacttgataa cgccttcgaa gccagcctgc gtagcgatga
     1441 aggaaacaag atcgttgaat tattcctcag cgatgaaggc gatgatgtgg tgattgaagt
     1501 cgccgatcag ggctgcggcg ttccagagtc tctacgagac aaaatatttg agcagggtgt
     1561 cagtacgcgt gctgacgagc ccggcgaaca tggcattggg ttgtacttga ttgccagcta
     1621 cgtaacgcgc tgcggtggtg ttatcactct cgaagataat gatccctgcg gtaccttatt
     1681 ttcaatctat attccgaaag tgaaacctaa tgacagctcc attaacccta ttgatcgttg
     1741 aggacgaaac gccgctggca gagatgcatg cggaatatat tcgtcacatt cccggattca
     1801 gtcagatatt actggcggga aacctggcgc aggcccgaat gatgatcgag cgttttaagc
     1861 cggggctaat cttgctcgat aactatcttc ctgacggtag agggattaat ttactgcatg
     1921 aactggtgca ggcgcattat cccggcgacg tggtgtttac cactgcagcc agtgatatgg
     1981 aaacggtgtc tgaagccgta cgttgtggtg tatttgatta tctcattaag ccaattgcct
     2041 atgaacggct ggggcaaacg ctaacccgtt tccgccagcg taaacatatg ctggaaagta
     2101 ttgatagcgc cagccagaag caaatcgatg agatgtttaa tgcctatgct cgcggtgaac
     2161 ccaaagacga actgccgacc ggcattgacc ccttgacgct aaacgcggtg cgaaaactgt
     2221 ttaaagagcc tggtgtgcaa catacggcag aaacggtggc gcaggcactg actatcagcc
     2281 gcaccactgc cagacgttat cttgaatatt gcgccagccg ccatctgatt attgctgaaa
     2341 ttgttcacgg caaagttggc agaccacaac gcatatacca cagtgggtga catgataaag
     2401 ccggaggaaa cttgcctccg gcatgactat ttacttgcct gtgaccgcgg ctgccgttcc
     2461 tggcaccatc agctctgtag caacgataac aatcaccagc ccaacaagca ccggcaccga
     2521 ggtgcgtttt acgacttcaa atggtgagat tttcgccatc ccggcaaccg caacgactac
     2581 gccagaaacg ggtgacaggg tacggccgag gtttgacgcc tgcagcattg ggatagtcaa
     2641 atacgccggg ttaatgccgg aagagtgcgc cagtttcggg atcatctcaa caaacgcata
     2701 aaacggcgca ttgcctgaac cggtcgtgac tgccgccagc attgtcagga tcaccaatac
     2761 cagcatcagg atgatgctcg ccgaaccaaa cgaggtagcg atagaaatca gactttgaat
     2821 aaagccgatg gtgctaagcc cctgagcgaa taccccagcg gcaaccagca gcatcaccac
     2881 gtttgcaaat gcatctgcca tcccgcgata agccacttcc agaccagaga aaactttctg
     2941 ggtattaaag ctgcggatga actccagaat ggaggcaatc agcatacaaa tcaccagaat
     3001 agtgatgatg tgtaattgcg gaccccattt gccgtcaaaa atcagcactc cgatgatcgg
     3061 cgtgaacggc aaaatggcat aaaacgcagg ggcagtggtg gtgatttcac tgacatctaa
     3121 catttcatga gagatgtgtt cttttttatc cagataacgt tgccagaaga agtgggcgat
     3181 tgccatgccg ataattgcag caattgagat aggcagcgtt gttttgaagg cgaagtcaat
     3241 cagcgacatt tcggaagcct gcgccgccag caccacatcc cctgaagtcg gtgcgagaat
     3301 aatcgccgcc ggggaggcac aaatagcagc agctgcacca cggctgatac caacgtttac
     3361 catcaccgga aacagggttg ccatcagcaa aacacccaga ccggtcgcgg aagagacggc
     3421 tagagacatc agacaggcga caaaataggc ggcaatcatc agcaggtaag gggagttaat
     3481 atactgcaat ggttttgacg ccaacttgac caccatatca ttcgcgccga tatgggtcat
     3541 gtaagcggca aagccacaca gcatcataat catcatgccg aggtcgccgc cgcggctcat
     3601 tagcaatatt ttaacgtatt caacgatatc cgtggcgctg tagcctgttg aagcctggct
     3661 ggacggtaac actttgtgcc ccataatggc actgataatc agcaataaca ggccaccgac
     3721 aaataacacg ccagtggcgg aatacccttt aatgatgtag cgagctacac ccacaataac
     3781 cacaacccca ataaggagtt caatgaatgt cagcataatt tttcctgtct ccaggcccca
     3841 aagtaaataa taaaaaattc ctaaagctta aggaaaaaat atgcccaata aattggcgat
     3901 gaatgctgat taaaatcaag aaaaactgcc attaagacat tgaagttgct gtttttatac
     3961 atagataaca aaacgcatat ttagcggttg gcaattatca cataataatg tgtgtaaatt
     4021 gatggtggat tgatgcaaat ttgttaattg caatggtgtt agctgcggct gtgcgctcaa
     4081 aaaataatct aatatgagca taggttgacg atatatatta tatacttcgt taagatgatt
     4141 gttgtatctc gttaaaaaat aaaataattt ttccttgatt gcattttgtc atcaaaaaag
     4201 acttgatttt tcttttttga ctattcccat cgcagaaaac gacg
//
LOCUS       DAZGHF010000245         4185 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGHF010000245 DAZGHF010000000
VERSION     DAZGHF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.247"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..786
                     /locus_tag="ACHK3A_004892"
     CDS             <1..786
                     /locus_tag="ACHK3A_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFO8742839.1"
                     /translation="ETDGKQRYEAWLMWLCDTFSVESGYAGLSFALPYARERMFPCEF
                     ALAQRFSGVMVDSLGTLEGGEAVEGLKGACWYTILGTPWLEKLGGAERVKHRLAKTPE
                     ISLLPYNNGVVLKAGELPPPLGEMKTEGLSPLLVKVNQVIRSIRYDGRNGLHFYSEYE
                     GLQFEEASSMAWFARFDEASALLDKEEMGKSCAPERIKCWTDEIAPHAGQWAAMVNGT
                     TEYIQTREGQKMPPFEDKHGKQHRACWKLLKRDDNGSVYTLPE"
     gene            800..1153
                     /locus_tag="ACHK3A_004893"
     CDS             800..1153
                     /locus_tag="ACHK3A_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO8742840.1"
                     /translation="MTVQAGERLGLFARTGQLSLKSGEGAVDVQAQNGSMRLFAEKKL
                     TLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTMATYLRRTKRTMVAAPATMPLP
                     IPLLLDLYPPLNGQI"
     gene            1482..1937
                     /gene="tssE"
                     /locus_tag="ACHK3A_004894"
     CDS             1482..1937
                     /gene="tssE"
                     /locus_tag="ACHK3A_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO8742841.1"
                     /translation="MSHHSPSLYETLYGNFTGGLDLHQVCEENQVIVSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTTILQGMPGTAHQLMRVLSDVLLKYEPRIKRVDVTMQEQTQ
                     PGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRHLKQQQFVDNSSYYHV"
     gene            2005..2310
                     /locus_tag="ACHK3A_004895"
     CDS             2005..2310
                     /locus_tag="ACHK3A_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742842.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRVFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            2365..2679
                     /locus_tag="ACHK3A_004896"
                     /pseudo
     CDS             2365..2679
                     /locus_tag="ACHK3A_004896"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            3004..3153
                     /locus_tag="ACHK3A_004897"
                     /pseudo
     CDS             3004..3153
                     /locus_tag="ACHK3A_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3328..3729
                     /locus_tag="ACHK3A_004898"
                     /pseudo
     CDS             3328..3729
                     /locus_tag="ACHK3A_004898"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            3711..3935
                     /locus_tag="ACHK3A_004899"
                     /pseudo
     CDS             3711..3935
                     /locus_tag="ACHK3A_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            3938..4185
                     /locus_tag="ACHK3A_004900"
                     /pseudo
     CDS             3938..4185
                     /locus_tag="ACHK3A_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1069 a          975 c         1128 g         1013 t
ORIGIN      
        1 gaaactgatg gaaagcagcg ttatgaagcc tggctgatgt ggttgtgtga caccttttcg
       61 gtggaaagtg gttatgcagg gttgtcgttt gcacttcctt atgcccgtga gcgaatgttc
      121 ccctgtgaat tcgccctggc gcagcgtttt tccggtgtga tggtggattc tcttgggacg
      181 cttgaaggcg gtgaagccgt tgaggggctg aaaggtgcct gctggtacac cattctcggt
      241 acgccgtggc tggaaaaact ggggggggca gagagagtaa aacatcgttt agctaaaacg
      301 cctgaaatta gcctgctacc ctataacaat ggtgtcgtac ttaaagccgg tgaactcccc
      361 ccgccactgg gagagatgaa aacagaaggt ttatcgccgc tgctggtgaa ggtgaaccag
      421 gtcattcgtt ccattcgcta tgatggacgc aatggtctgc atttttactc agaatatgaa
      481 ggacttcagt ttgaagaagc gtcttcaatg gcatggtttg cccgttttga cgaagccagt
      541 gccttgctcg ataaagaaga aatggggaaa tcttgcgccc ccgaacgcat taaatgctgg
      601 accgatgaaa tagcgcccca tgccggacaa tgggccgcga tggttaatgg caccaccgag
      661 tacatccaga cccgcgaagg gcagaaaatg cccccgtttg aggataagca cggtaagcag
      721 cacagggctt gctggaaact actgaaacga gatgacaatg gtagcgttta tacactacct
      781 gagtaaggtc gtgtgctgaa tgaccgtgca ggccggagag aggctggggc tttttgcccg
      841 caccggtcaa cttagcctga aatccgggga aggggcagtg gatgttcagg cgcaaaacgg
      901 cagcatgcgg ttgtttgctg agaagaagct gacgctgagc tcagcgagtg acatctcctt
      961 tgccgggaaa aaacgcatca cgctgattgg cgggggaagt tacctacggc tggaggcggg
     1021 gaaaatagag tacggtacga tggcgacgta tctgcggcgg acgaaacgga cgatggtagc
     1081 agccccggca acaatgccgc tccccatacc gcttctcctg gacctgtatc caccacttaa
     1141 tggtcaaatt tgaattgaat gtctcctcaa tgccatcaaa gtcaacgatg ctatcgttca
     1201 gggagcgtag tcaatggatg tcacaaaaat gatgcaggtg ctacaggatt tgtttttatg
     1261 ctgctaaatg tacagcgact tcgtgatatg gggttgttgg tgttttttat aataatgctt
     1321 tttacggaat caaaaaaaca gacaacatat taattatttg agaaagcagc gtcaaaaagg
     1381 aaaagttaaa atgtcgaatc gattttttac tccggtaatc ttctttccag tgtgttgatt
     1441 gaactatttc caagattaaa tgattatgag aagaagtcgt gatgtctcat catagcccct
     1501 ccctttacga aaccttatac ggaaatttta ccggcggcct cgacctccat caggtctgtg
     1561 aagaaaatca ggtcatcgtg tcggtgctcg ataacatgca gcgtattctg aatacccggg
     1621 ccggaagcct taaacatctt ccggactatg ggctgccgga tatgacaacc attctgcagg
     1681 gaatgccggg tacggcgcat caactgatgc gcgtgctttc agatgttctg ctgaagtatg
     1741 agccacgtat taagcgtgtc gacgtgacga tgcaggaaca gactcagcct ggtgagttgc
     1801 attatgtcat tgatgcagaa cttaaagatg ccgggctggt tcgttacgga acaacattta
     1861 tgccggaggg cagagtgttg ctgcgccatc ttaagcaaca gcagtttgtg gacaattcat
     1921 cttattatca tgtctgattt ttttatccgg gctgcccctg tgtgggtggt ccggatgaaa
     1981 aatatatttt ttgtgatttt gattatgaac ccgttattta acgatattca gatgcgtctt
     2041 ttttatctga accactctcc gtactcctgg cactggaatg tcagattcag acctcaggaa
     2101 gccgtatata ttggcagcga tacctgtcat atcaccatta cctgtaatca gtccggtttt
     2161 catttaaccc gggacggaca acgggtattc actgagcgtt atatcagaaa ccttaatgag
     2221 ctgttaccag ttctgaaacg gcggtgggat gtgacaccgg caatcatccg tgcagtggaa
     2281 tacctttccc gggtcccggt atcacactga cattctttcc gtaactgtta atcatgattt
     2341 aatacagcaa cactgaatct gccgatgact tctgaagtga aactgaaaac aggtggtgac
     2401 ccccggtcat tcccggatta tgctgccctg cgcgatgaaa tcagcaaact gacccacccg
     2461 gcacgcccgg atgtggactg gcgttatgtg gaaacgctct gcctgcgcct gtatgaacac
     2521 aatggcgtgg aattacagac agcatcctgg tacaccattg cccggatgca tacaacaggg
     2581 ttaagtggcc tgaatgaagg gctggctctg attgtggcgc tgacccggca tcactggtca
     2641 gtgatgtggc cgctgaatac ccacgcccgg ctggaaatta ttaccggtct gttcaaccgt
     2701 ctgcaaaaaa cgctccgggc catgccaccg gacgaccgtg acaacctgcc cctgctgtat
     2761 cagaccgaaa cattcctgaa ggcactgagt gatacactgg cccggcacga acttaaacag
     2821 agcagtaaag tggctttacc ggaaaccatg gtgaagggat atatcacccg tctggaaaat
     2881 cagccgatgc agggcgaagt gtcgtctccg gtaacccttc ctgctcgggc actccggtct
     2941 gatgctccgg atgtacaggg acatcagccc ctgccgcact cccgtctggt gtatattgtt
     3001 tctgaaacaa aagcagagcc cacaccctcc cttcaaaaat ccccgcccac gtttctgaaa
     3061 ccgtttgtgg ctggtgtctg tgcggcattg ctggcggtca gcgtggccat tccgggatgg
     3121 cagtttctga cacagccatc accggcggag cagcaactca gggcgctgct ggcaccacta
     3181 ccggatatgc tttcagcaga ccagatggca cagctcagca gaacaccctc actgttaaat
     3241 catgcgtcag actggataac actttcaggt cagcagataa cgcgactgac agaactaccg
     3301 ctgacataca acctgcagcg tagtgcgcag ctgttgcagc aactgatggt acttttcccc
     3361 gataatcctc gtgtacagga aatggtggat aactggcaga agagcgtaag gagtcgtgcg
     3421 ctcccggaag aggcgatgac gggctggaat gaaggcatga cccgcttaca gcagctggct
     3481 gaacgcctga accgcctgga tgaacagcgg ggaaaataca tgacggtcag tgaactgaaa
     3541 acggaggtgt ttggcatcat gcaggctttt aaccggcaca taccggcaga agagcagtta
     3601 cgtcgctatg atgaagtccg taatcagaat ggcagcgaat ctcagcagaa actggcgcag
     3661 gatgctctga tggaacagtt gaaccgctac tggcttctga gacatggaga tgctgggaat
     3721 ccggcgtgac ttgggttcgc caaccaccgg aagacgccat tacggatatg gcgtattttc
     3781 ccttttaccg gtatgccatg gcctttgaag gattcattcc ggcattttca tcttggtttg
     3841 caggtaaccg gtgtggagtg gcaatactga ccggtgtccg ggcggatgaa tccctcaact
     3901 gctttatggg gctggtgtct cagcgcaaac tgagagaatt acgtgggtag gatcagatga
     3961 tggtattgga actgaaggtg atatgggaaa atggttgtta ttattttgtc tgctgtagct
     4021 cagtctgaat gacaacatat acttgaaaga acaaatgaag gccgtctcga agccgaagta
     4081 aagggtattt aatttgggcg aaaaagaacg gtcaacaggg aacaagtaca aaccttacga
     4141 agtctgggta tcggcgctac agaaatttcg cgaaagatga aattt
//
LOCUS       DAZGHF010000246         4125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGHF010000246 DAZGHF010000000
VERSION     DAZGHF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.248"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            63..2099
                     /locus_tag="ACHK3A_004901"
     CDS             63..2099
                     /locus_tag="ACHK3A_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO8742843.1"
                     /translation="MNIRNNHLPGTETLFSETETANSKQLAVLKTNFPQCFDKNGAFI
                     QEKLLEIIRASNVEISKESYCLNWLGKSYARLLANLPPTTLLSEDKDHNRREENKNSQ
                     NLLIKGDNLEVLKHMVNAYSEKVKMIYIDPPYNTGSDGFAYNDDRKFTPEQLSDLAGI
                     DLDEAKRILEFTTNGSSSHSAWLTFIYPRLYIARELLREDGVIFISIDENELNQLKTI
                     CDEIFGEANFIENIVWNKRIPKNDKGIGNIHEYILAYAKNNEISKEFLMRKDGIDDVY
                     QFIEKLKKEKVPLDKAEQQLKKFYSSNGYDRGITLYNGLNEDYRPWGKINMSWPNADS
                     FGPTYEVLHPLTNNPVKIPDRGWRWKEGTFNHIAKRIDGKYADIKKLHDGSVICGGIW
                     FARTEKTQPSSVKFLDEVEEFLLRSIISTKSDGGVEVENLFGGKGYFSYPKPTSLIKT
                     LFGSVKTEDKDIYLDFFAGSGTTAHGILELNIEDGRKRNFICVQLDEETDKKKQAYKE
                     GYKSIFNITKDRIIKAGKKLKKDNPNYNGDLGFKIFETVHDFRAKDESELTLSNLSFF
                     DDAVLTPEQYDTLLTTWCVYDGSLLTTPIEDIDLNGYKAHLCDGRLYLIAPNFTSEAL
                     KALLQKLDADKDFAPNKVVFYGSNFESAKQMELNEALKSYANKKSIDLDLVVRN"
     gene            2102..>4125
                     /locus_tag="ACHK3A_004902"
     CDS             2102..>4125
                     /locus_tag="ACHK3A_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO8742844.1"
                     /translation="MSKGFTFEKNLPHQKAGVDSVMNVFVSAIPHQTDHVAIRLLANP
                     ELNLSEQQYYNNIKNVQEFNGIEHSKDNYDAKSNVIDVSMETGTGKTYTYTKTIFDLN
                     KSFGINKFIIIVPTLSIKAGTVNFLKSDAIKEHFRDDYERELKTYVVESQKSSGKNTK
                     SYMPQAIHDFVEASNFNKKYIHILVINSGMINSKSLTDTYDVGLLDNQFDTPFSALSA
                     IKPFIIIDEPHKFPTGKKTWEHIQKFNAQYIIRYGATFSEGYKNLVYRLTAVDAFNED
                     LVKGIDAYIEDIVGDGNANLKLVKSDGKEATFELNENNNKKIFKLAKGESLSKTHSAI
                     HDLTLDALNKNTAVLSNGIELKIGSSINPYSYDQTLADNMMRKAVKEHFKLEKEYLTQ
                     RPRIKPLTLFFIDDIEGYRDGNDIAGSLKTKFEEYVLAEANELLKTEKDEFYRNYLEK
                     TVKDISSVHGGYFSKDNSDKDDKIEQEINEILHDKELLLSLENPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNEYMCRVKNRDFTLNYYVDFTEKDFV
                     DSLVKEVNDSSFKETVPGKFTQELKDKILSQYPELSSRTLMNEIFDAEIIDDNENFKD
                     SDAYSRLKAKYPAAFPAGVKPSKIKKATDGKKRTKMRIGKFSELKELWDL"
BASE COUNT         1430 a          692 c          800 g         1203 t
ORIGIN      
        1 atatgctcgc ctgaactctc tccgtcaagt acctctacct ccgtgctttc tggaggtgct
       61 gtatgaatat acgcaacaat catctaccgg gaacagaaac tcttttttct gaaactgaaa
      121 ctgcgaacag taaacaactt gctgttttga aaactaactt tccacagtgt ttcgataaaa
      181 acggagcttt cattcaggaa aagctgcttg agatcatcag agcatcaaat gttgaaatct
      241 ctaaagagtc atactgctta aactggcttg gcaaatccta tgctcgcctg ctggctaacc
      301 taccaccaac gacattgctg tcagaagata aagaccataa cagacgtgaa gagaacaaga
      361 acagtcaaaa tctgttaatc aaaggggata accttgaggt attaaagcat atggttaatg
      421 cttattctga aaaggttaaa atgatttata tcgatccgcc atataacacg ggttctgatg
      481 ggttcgccta taatgatgat cgcaaattta caccagaaca gctttctgat ctggcaggta
      541 tcgatcttga tgaggctaaa cgtatacttg aatttaccac gaatggttca agcagccata
      601 gcgcatggtt aacgttcatt tatcctcgtt tatatattgc tcgtgagctt ctacgtgaag
      661 atggtgtgat atttatttcg attgacgaaa atgaattaaa tcaacttaaa actatttgtg
      721 atgaaatttt tggcgaagca aatttcatag aaaatattgt atggaataaa cgtatcccta
      781 aaaatgataa gggtattggt aatatccatg agtacatcct tgcttatgcg aagaataacg
      841 aaataagtaa agaattttta atgcggaaag atggtattga tgatgtatat cagtttattg
      901 aaaagttaaa aaaggaaaag gtgccattag ataaagcaga gcagcaactc aaaaaattct
      961 acagctccaa cggatatgat cgtggaatta ctctttacaa cggtttgaac gaggattata
     1021 ggccatgggg taaaattaat atgagttggc ctaatgcaga ttcatttgga cccacgtatg
     1081 aagtattgca ccctttgact aataaccctg tcaaaatccc agacagaggc tggcgttgga
     1141 aagaaggcac ttttaatcat attgcaaaaa gaatagatgg aaagtatgca gatatcaaaa
     1201 aactacatga tggtagcgtt atttgtggtg gaatttggtt tgcccgtaca gaaaaaaccc
     1261 aacccagttc ggttaaattt ttagatgaag ttgaagagtt tttactaaga tcaattattt
     1321 caactaaaag tgatggtggt gttgaggttg aaaatctttt tggtgggaaa ggatatttct
     1381 cctacccgaa gccaacaagt ttaataaaaa cactatttgg ttcagtaaaa accgaagata
     1441 aagacattta tcttgacttt tttgctggct ctggaactac agctcatggt attctggagt
     1501 taaatattga agacggcagg aaaagaaact tcatctgtgt tcaattagat gaagaaacag
     1561 ataaaaagaa acaagcctat aaagaagggt ataaatccat cttcaatata acaaaagaca
     1621 gaattattaa ggcaggaaaa aagctgaaaa aagataatcc taattataat ggagatttgg
     1681 gatttaaaat atttgaaaca gttcatgatt ttcgagcgaa agatgagtct gaacttacgc
     1741 tttctaatct tagtttcttt gacgatgctg tattgacacc agagcagtat gacactctat
     1801 tgacgacatg gtgcgtgtat gatggcagct tgctaacaac accaattgaa gatattgacc
     1861 ttaatggcta caaagcacac ttgtgcgatg gtcggctata tctaattgcg cctaatttca
     1921 caagtgaggc tttgaaagct ttacttcaaa agctggatgc agacaaagac tttgccccta
     1981 acaaagttgt attttatggc agcaacttcg aaagtgcgaa acagatggaa ctcaatgaag
     2041 cactaaaaag ttatgctaat aaaaaatcta ttgatttaga tttggtggta aggaactaat
     2101 catgtctaaa gggttcacat tcgaaaaaaa tttacctcat caaaaggctg gcgttgattc
     2161 ggtaatgaat gtctttgtat ccgccatacc tcatcagacg gatcatgttg caattcgcct
     2221 gttagctaat ccagaactga atctatctga acaacaatac tacaacaaca taaaaaatgt
     2281 tcaggagttc aatggtattg agcactcaaa agataattac gatgctaaaa gcaacgtaat
     2341 tgatgtttct atggagacag gtacaggtaa aacttatact tacaccaaaa caatttttga
     2401 cctgaacaaa tcatttggta ttaacaaatt tatcatcatt gttcctaccc tatcgatcaa
     2461 agccggaacc gttaactttt taaaaagcga tgctataaaa gaacactttc gagatgatta
     2521 cgagcgtgaa ctaaagactt acgttgttga aagtcagaaa agttcgggta aaaatacaaa
     2581 atcgtatatg cctcaagcta tccatgattt tgttgaagca agtaatttca ataagaaata
     2641 tatacacatt cttgtgatca actcagggat gattaactct aagtctttaa ctgacactta
     2701 tgatgttggc ttgcttgata atcagtttga taccccattt tctgcactta gtgcgataaa
     2761 accattcatt atcattgatg agcctcataa atttcctact ggtaaaaaaa catgggaaca
     2821 tattcaaaaa tttaatgccc aatatatcat ccgctatggc gcaaccttta gtgaaggcta
     2881 taagaactta gtttatcgtc ttacagccgt agatgccttc aatgaagatc ttgttaaagg
     2941 tattgatgca tacattgagg atattgttgg tgatggtaat gccaacctca agttggtcaa
     3001 atctgacgga aaagaagcta catttgaact gaacgaaaat aataataaaa aaatttttaa
     3061 gttagccaaa ggtgaatcac tatctaagac gcatagtgcg atccatgatt taactcttga
     3121 tgctttaaat aagaatacag cagtgttgag taatggtatt gagctaaaaa ttggtagctc
     3181 gataaacccc tattcttacg atcagacact tgctgataac atgatgcgta aagctgtcaa
     3241 agaacacttt aagttagaaa aagagtatct gacacaaagg ccacgcataa aacctcttac
     3301 tcttttcttc attgatgaca ttgaaggcta tcgtgatggt aatgatattg caggaagcct
     3361 gaaaactaag ttcgaagaat acgttttggc agaagcaaat gaactgttaa aaacagagaa
     3421 agatgagttt tacagaaact accttgaaaa aactgttaag gatatatctt ctgttcatgg
     3481 tggttatttt tcaaaagata atagtgataa agatgataaa attgagcagg aaatcaacga
     3541 aatccttcat gataaagagc ttctattgtc tttagaaaat cctcgccgtt tcattttctc
     3601 taaatggacg ttgcgtgagg gatgggataa cccaaatgtc tttcagattt gtaagctccg
     3661 ttcgagcggt agcaccacat ctaaacttca ggaggtaggg cgtggtttac gtcttccagt
     3721 gaatgaatac atgtgccgtg tgaaaaaccg cgattttact ctcaactatt atgttgattt
     3781 tacggaaaaa gattttgttg attctcttgt aaaagaggtt aatgatagtt ctttcaaaga
     3841 aactgttcca ggtaagttta ctcaagaact taaggataaa atcttatctc agtatcctga
     3901 gctttcatcc agaacactca tgaatgagat ctttgatgct gaaattattg atgataatga
     3961 aaacttcaaa gattccgatg cctatagccg tttaaaagca aaatatccgg ctgcattccc
     4021 tgccggggtg aaaccaagca aaataaaaaa agccacggat ggaaaaaagc gtactaaaat
     4081 gcgtattggt aagttcagtg agttgaaaga actatgggat ttgat
//
LOCUS       DAZGHF010000247         4110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGHF010000247 DAZGHF010000000
VERSION     DAZGHF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.249"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..488
                     /locus_tag="ACHK3A_004903"
     CDS             <1..488
                     /locus_tag="ACHK3A_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8742845.1"
                     /translation="SYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEKKAGSIDGVRS
                     NEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRETGDKYGFTGEH
                     HESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSAVILNLVGECE
                     Q"
     gene            567..992
                     /locus_tag="ACHK3A_004904"
     CDS             567..992
                     /locus_tag="ACHK3A_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO8742846.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIKLEMDRYVERERSGAQVSQFSGHTGN"
     gene            1192..1347
                     /locus_tag="ACHK3A_004905"
     CDS             1192..1347
                     /locus_tag="ACHK3A_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8742847.1"
                     /translation="MKQQKAMLIALIVICLTVILTVLVARKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1475..3631
                     /locus_tag="ACHK3A_004906"
     CDS             1475..3631
                     /locus_tag="ACHK3A_004906"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFO8742848.1"
                     /translation="MCECSKNVDFHKRTILCCQTSDQRPATSDQRPATSDRKSRKSVK
                     SVEPDIADRANSWLKSYGLDYKLEQAPLNNEIDNALDAYFSKNGGAGGNRPDVKLLVQ
                     DKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKEWNFKNINGYAVNGAVHYANAL
                     LQFTNYPDIIAIGMTGWRDDDTGELHHEIGVWYVSKNNLGAGQKVGEFTDLSFLADKN
                     VDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLNNDIYQNEKGLGESDRVYLVVA
                     TVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRKIRNFLRLKAVPETKREMILRS
                     LQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKIGLTTDFTGKLFNEMYRWLGFT
                     QDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGSAGLLVAAMNEMLIDARENIHS
                     PNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMGDGSSNILNKDSLADFDGKYGF
                     GKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDKGYAAVIIQSSAGTGKATEYNK
                     KILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIPHNAKQAVKFIDFSNDGYARSN
                     RKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTEDEYFEGTIDPDSGEDWNQTRP
                     VDTRPTLEDFKKTVGDYLAWEVSKLLQGRDASEKKQ"
     gene            3664..>4110
                     /locus_tag="ACHK3A_004907"
     CDS             3664..>4110
                     /locus_tag="ACHK3A_004907"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO8742849.1"
                     /translation="MKSGGKWEEYTIERLFSIQTPKKKFNANKLTFNGNYPYVARGSN
                     NNGIKGYIDEDELYLNPGNTISFGQDTATMFYQENAYFTGDKIKIFSLRHGELNSRIA
                     VFLISAMKKSFSTFSWGSSSFNENVLNKVKINIPTFNNVPAYNYMVK"
BASE COUNT         1215 a          795 c         1055 g         1045 t
ORIGIN      
        1 gcagctatga ggcagcactc gacgatgtgg aggctgagcg gataattcgc acctccccga
       61 aatttatcga cgattactac tggggctgga ctgcggcaga gaaaaaagcc ggaagtattg
      121 acggtgttcg ctcaaatgaa attgatgatc agcgtcgtgc atatgttgat ggctatcgtg
      181 atgtactgcc agagcctcat acattgtcag acgttgttcg tgagtttatt tactgggact
      241 ggctttatga gatgcgccag acagcgggga gagaaacagg ggacaaatac ggtttcacag
      301 gtgaacatca tgagtcagta tatgatcggc agttctggct cgaaaatttg ctgggaaaaa
      361 taaagcctgt gacgcgtgat gaagcggttg aagtgtgtcg ctggtttctg gcaagcggaa
      421 aagatgagta catggaagac aatggttcag cggtcattct caatctggtt ggggagtgtg
      481 agcaatgaag ccggagatat ccagtagaca ttttccccct gagcattcaa atcagaaaaa
      541 gtatggttac agcggagtac agaataatgc gtgatatcca acaggttctg tcccgatggg
      601 gtgcatgggt ggtaaataat catgagagtg tggcatggtc cggcgtcgct gccggattta
      661 agggcgtgat cccatcaaag gttaaatcac ggcctcagtg taccgatgat gatgcgctga
      721 ttatcagcaa ttgcatggcg caactgaacg tcaacaacag cgatttgcat gattttctgt
      781 atgattatta tgtgttcggg atgacgctta tgggactggg ccgtaagcat gagcgctctg
      841 attgctgggc cgggcgggta ctgcaaaaag ctgaaggtgt tattgagggg atgttgatta
      901 tgcagggaat aaaactggaa atggacagat acgttgagcg tgaacgatca ggggcacagg
      961 tcagtcagtt ttccggacat acgggaaatt gaaagcgcgg ggttttactg tagaatgact
     1021 gcgggtgctt gagggtgtct gcctcgggca tgctgccgta aggcagacgg agaaaagccc
     1081 cagttaacat tacgcgtcct gcaagacgct taacattaat ctgaggccaa tttcatgctg
     1141 aacacatgta ggttagcctc ttacgtgccg aaaggcaagg agaagcaggc tatgaagcag
     1201 caaaaggcga tgttaatcgc cctgatcgtc atctgtttaa ccgtcattct gacggtactg
     1261 gtagcgagga aagacctctg cgaggtacga atccgaaccg gccagacgga ggtcgctgtc
     1321 ttcgtagact acgaatctga gaagtaagag acctggcggg ggagtaatct cccgccccct
     1381 ctgatgtgtc aggcatcctc aacgcacccg tactttagtt gtcatatgct ccgcaaaatg
     1441 caggttgtcg ttgcaacccg tgcggtaatt ttttgtgtgc gagtgctcaa aaaacgttga
     1501 ttttcataaa aggacgattt tatgctgcca gaccagcgac cagcgaccag cgaccagcga
     1561 ccagcgacca gcgaccagcg accgtaaatc aagaaagtct gtaaaatctg ttgaaccaga
     1621 cattgccgac cgggctaaca gctggctcaa atcttatgga cttgattaca aactggagca
     1681 ggcaccactt aacaacgaga tcgataacgc actggatgct tatttttcga aaaatggcgg
     1741 cgcaggcggt aatcgtcccg acgtaaaact ccttgttcag gacaaatacg gcaagcagta
     1801 tcccgttctc atcgagtata aaggctacaa ggacaggctt atcagacttg gcagcaacgg
     1861 tattattgag aacaaagatg ccagaaaaga atggaatttt aaaaatatta atggatacgc
     1921 ggtgaatggc gcagtccatt atgccaacgc acttcttcag ttcaccaatt accctgatat
     1981 tattgctatt ggtatgaccg gctggcgcga tgatgataca ggcgaactgc atcatgaaat
     2041 cggcgtgtgg tacgtatcaa agaataattt aggagccggg cagaaggttg gtgaattcac
     2101 cgacttatca ttccttgctg acaaaaatgt tgatggattt ctcaacaaga taaagctgct
     2161 taatcttcct cccgaagaac tggaaaaaat caaggccagc aaagaggaag agatagacac
     2221 gcgactttcc cgtctgaata acgatattta ccagaatgaa aaggggcttg gtgaaagcga
     2281 tcgtgtttac cttgttgtgg ctaccgttat tgcaacgttg gggattccgg gaaagttagc
     2341 accgctggat aaaaaagaac tgacctcttc aacggaggag gacttgcgtg atggtgatat
     2401 tattttcagg aaaataagga attttttaag gctgaaagct gttcctgaaa caaaaagaga
     2461 aatgattttg cgctcgttgc aaaatacgct gtggactgaa aacattaaca aaccagtcaa
     2521 cggtgaaagc cagttaaagc gcgtgtttgt taaagttgtt gatgatcttg gggagtatta
     2581 caaaatcgga ctgaccaccg attttacggg caaactgttt aatgagatgt atcgctggct
     2641 tggatttacg caggacaaac tcaatgacgt cgttctgaca ccaccttatg ttgccacatt
     2701 gctggcacga ctcgccagag taaacaaaga ttcctatgtg tgggattttg ctacaggttc
     2761 tgcgggattg cttgtggctg caatgaatga aatgctcatt gacgccagag aaaatattca
     2821 ctcaccgaat gaactacaac ttaaggaagc gcaaatcaag gctgaacagc ttcttggcct
     2881 tgaggtgtta tccagtattt atatgctggc catcctgaat atgattttga tgggggatgg
     2941 tagctcgaat atcctcaata aagactcgct ggccgatttt gacggcaagt atggatttgg
     3001 gaaaacagga gaaaaattcc ctgcggacgc atttattctc aatcctccgt attcagctaa
     3061 aggtaacggc atgattttcg tgcagaaggc gctgtcgatg atggataaag gctatgctgc
     3121 agtgattatc cagagttcag ccggcacagg aaaggctacg gagtacaaca aaaaaatact
     3181 caaggaaaat accttgctgg caagcatcaa aatgcctgcg gatctgttta ttggtaaatc
     3241 aagcgttcag acgtacattt acgtttttca ggtaaaaatt ccgcataacg cgaaacaggc
     3301 tgttaagttt atcgactttt ccaatgacgg ctatgctcgc tctaatcgca aaaaggcaag
     3361 aaacaatctt gttgatgccg atcgtgcaaa agagcgttat caggaggttg tggatctggt
     3421 tcacttcggg aaaggttgcc ttaatatctt tacggaggat gaatattttg agggaacaat
     3481 cgatcctgat agtggtgagg actggaatca gaccaggcca gtagatacca gaccgacact
     3541 ggaggatttt aaaaagacgg ttggcgacta tcttgcgtgg gaggtgtcaa aacttcttca
     3601 ggggcgtgat gcgtcagaaa aaaagcagta aactcccgtc ttgcagagct tgagcgggag
     3661 tttattaaat ctggtggtaa atgggaagaa tacacaatcg aaagattgtt cagtattcag
     3721 acacctaaaa agaaatttaa tgccaataaa ttaacattta atggcaacta tccttatgtt
     3781 gccagaggaa gtaacaataa cggcataaaa ggatatatag atgaagatga attatatctc
     3841 aacccgggga atacaattag ctttggtcag gatactgcga ccatgttcta tcaggaaaac
     3901 gcatatttca caggtgataa aataaagatt ttttctctca gacatggaga gttaaattcg
     3961 cgaattgctg tttttcttat atcagcaatg aaaaaatctt tttcaacgtt tagttggggg
     4021 agtagctctt ttaatgagaa tgttctcaat aaggtaaaga taaatatacc tacgtttaat
     4081 aatgttcctg catataatta tatggtaaag
//
LOCUS       DAZGHF010000248         4062 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGHF010000248 DAZGHF010000000
VERSION     DAZGHF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.250"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(126..1103)
                     /locus_tag="ACHK3A_004908"
     CDS             complement(126..1103)
                     /locus_tag="ACHK3A_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFO8742850.1"
                     /translation="MKTKKHTSRTATKTPERDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEMMPANALTGKLYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGNRLTDSEGEPLMEIVPVLKSNPLFNVGQCDGLPEHL
                     MVKAEGSADALPADTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQ
                     VADYWTTLLHELVHSTGHEKRLRRVGISTFTGRNSPEYAFEELIAEMGSAFLCAHLGI
                     AGDVQHESYIDGWLKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
     gene            complement(1196..1579)
                     /locus_tag="ACHK3A_004909"
     CDS             complement(1196..1579)
                     /locus_tag="ACHK3A_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742851.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            complement(1695..2324)
                     /locus_tag="ACHK3A_004910"
     CDS             complement(1695..2324)
                     /locus_tag="ACHK3A_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742852.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFSAPESVL
                     TTPVRGDAFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRALAGGR"
     gene            complement(2406..2783)
                     /locus_tag="ACHK3A_004911"
     CDS             complement(2406..2783)
                     /locus_tag="ACHK3A_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742853.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            complement(2979..3458)
                     /locus_tag="ACHK3A_004912"
     CDS             complement(2979..3458)
                     /locus_tag="ACHK3A_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742854.1"
                     /translation="MHSLLILLYVVVWRACTILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWEDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            complement(3541..3933)
                     /locus_tag="ACHK3A_004913"
     CDS             complement(3541..3933)
                     /locus_tag="ACHK3A_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742855.1"
                     /translation="MSTFILYPEPGISSFFRRMTAQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLRYRFGLQGDGIVAQPC"
BASE COUNT         1064 a         1190 c          947 g          861 t
ORIGIN      
        1 ccattcagac attgtttacg ctcctctttt ttatacagat gtaaaaagag ggcattgtcc
       61 cgtgaggtac aatgccctca cgggataaat ggcggtcagg ttataaccgc caccggtagt
      121 aaaaattatg ctgcgcactc ctgcgaagtt cgccgcgtca gtaaatactc tgatgcttca
      181 cgtgcctgcc ggcaggcgcg aaacagcgct tttttgtcag acttcagaac cttaagccat
      241 ccgtcaatat aactttcgtg ctgtacgtca ccggcaatcc cgagatgtgc acacagaaaa
      301 gcactgccca tctcggcaat cagctcttca aacgcatatt ccgggctgtt gcgaccggta
      361 aaggtactta tccccacccg ccgcaggcgc ttttcatgac cggtactgtg taccagctca
      421 tgcagcaggg tggtccagta atcagccacc tggtcaaact gcgacgacag tggcatcacg
      481 atacggtcaa gatcaggccg gtagaatgcc ctgtcctgtg gaagtgacga gacgctgacg
      541 ccgctgccat tcagtacctg cagcacccgc tcactgaagg tatctgccgg cagtgcatcc
      601 gcgctgcctt ccgccttcac catcagatgt tcaggtaaac cgtcacactg ccccacatta
      661 aacagaggat tcgatttcag taccggtacg atttccatga gaggctcccc ctcactgtcc
      721 gtcagccggt taccgtgttc atccacagcc tgttttttcc agtctttgta aatcaccgcc
      781 agcgtggcca cctccccttt acggacgtgg ccacccagtt gttgcgcctg acggtacgtc
      841 agccagtcat tgctgacata cccggcttca tccgcggcca gccacagcaa caggacgttc
      901 acccctgtat aaagcttccc ggtcagggca tttgccggca tcatctccgg aagccccacg
      961 ccattacgcg cagagcgcca gggacgtttc cagggtggca caccattttc aatggccgta
     1021 acaaccctgt cggtcacaag ctgatacagg tcacgttccg gagttttagt cgcggtacgt
     1081 gaagtatgtt ttttcgtttt cataagagca tgccccctga aaaagatgca cgctccctgt
     1141 acggtgagag tgcaccgtac agttaaatta ataacgaaaa tcagactggc cagaattacc
     1201 acaacgccag catcaccagt gaacgacgct caaaatcaaa cgtcttcagc gtattgctga
     1261 cccacgcttt tacgtcatcc acgtcatacc agtaatcacc ggcataatct tccccccgta
     1321 aacacgagcc tgtgacataa ggaaacagcc tgtggcagtt ccccggtgtc agcatactga
     1381 ggtcgcgcaa taatgcagcc agtgaactgc gggttacagg gaaagcatgc tgctcatctg
     1441 aagcaccggt atgttcacga aaccatgtca gaagcggttt caccttacgg aaagccccca
     1501 cctcctgatg ttcctgtgtt cccacaacgg caccactgtg atggttatca cgagccagaa
     1561 agtaaatatc gagtcccata acaattctcc tgaataaagc cgggactctc cccacaggga
     1621 tatgtccctg ccgggtgata taatgacgga atacgtgatg acgttattcc ggataaagaa
     1681 actacggcgt tctgttaccg cccaccggca agagcccgca gccagtaaag ctgatattcg
     1741 gcctcttcca gtgctgccca gtcatccggg gataactgtc gcccctgacc ggacaaaaac
     1801 tgcagcagta agctgtcaaa cgcatctccc cttaccgggg tggttaatac agactccgga
     1861 gcagaaaaat tctcatgccc caggtaccgt tttatccatt tcagcggcag cgaccagctg
     1921 gcaccaccga cagtggaatg aatacgaaaa cagtccggac acagattgtg caacagcaca
     1981 aagactgtac gggcaaagaa atctcccggc tgacgtccga aattcagctc attaagagcc
     2041 aggatattcc ggctgtgctc ccgctcaata gtgacattca ccgaccacag cccgtcggca
     2101 gaccggcagt aaaacgccag cgtgtccgtt accggaaaca actctgacgc ccgggtcagg
     2161 gagccgaaat acgcattctg cggccgtaac gcctccggca actgccagaa ggtttcagtg
     2221 acagccagaa caaaagcatg ccactgcttt tccggtattg tggaaacctg ctcaagggta
     2281 tataccaccg gctcacgggc cggacgtgaa gataactcag acatggaaca ttcctcatgg
     2341 ttaaaacgaa acaacgggga atgtctcccg gacggagaac tcccccgtca gggtcaggtc
     2401 atgaattaca tcatgtcgtg ccgggcaaaa tattcatccg ctccggccac catctttccg
     2461 gtactgatac tgtcagccca gatttcatga ttaatttcac gccccagagc catcagactg
     2521 tcgagtgcct gacgggcctg aattcccggt gcggaaatct cctcctgctc caggtcatac
     2581 aacagccagc ccagcgcctc gccggtatcc cacagataca cgtataccag tgaaggctcc
     2641 gtctcatcat ccggctccgg ggtaataccc agccgttgcc aggcacgtaa ccgcatcagc
     2701 tcgccttcca tctgactgat atgagccagc agagcagact gcgtctgaag cgctgaataa
     2761 tgagcaatta aaggtgaata catgatgacc tccgtctctt aaaggaagtc ctgtccctga
     2821 cggaacaaaa cttccgtcag ggaaaacacc ggagcaaaaa cacggtgcag taagtaaaag
     2881 aaaaaccgtt acgggagcaa aacagccatc attgcaaaat ggcagttgca tcgctcacga
     2941 tttgacatcc ttcctgtgac tgactggcat cctgcccgtc agtctgcgtc ggaaaattca
     3001 atacaggcct gcttcaggag cccggctata cgttgattca gacattccgt ccagccatcg
     3061 ttccatccac gctcccatgg attgcagaca tcctcccatg gattgcagac atcgttacag
     3121 ccattcttcc atgtcttcca tgcctccatc tccatgacag cccccagcct gtggcctttc
     3181 acacgtatat gccacagaaa cggccggaaa agtcctgatt tcagttggtc accaccgctt
     3241 ttgtagccca gatgacgtgc aatactttca gtatattcaa atgcatcatt ctgcattcca
     3301 gccgccttag ccagcgagct gtacgcatca cgaagtatcg tcataccttc cagttgatac
     3361 tcacaccacg cacggacagc cgacactggt gcgattaccc gaagaatggt gcaggcacgc
     3421 catacaacaa cgtaaaggag tattaacaga ctatgcatgt gttcacctca gatacaaaaa
     3481 ataacgggaa cacacctgcc ctgcgggaag cgtgttcccg acagggtaat aaatgataaa
     3541 tcagcagggc tgtgcgacga ttccatcacc ctgcagacca aaccggtaac gcagggtggt
     3601 cagttgctcc gccatacggc aggcagcatg ccatgtcctg cgacgggata tccgtccggt
     3661 aaaccggttc agataaatga cctcacgcgg taacggctga taccatcctg tgtactcgtt
     3721 gcgatacatt ccctgccggt aatacaggca gggtgtccag acaccgccac gacggaacat
     3781 acagacccgc acgctcagat tgccggccgg attagtgtac cagcgactgt aatatgacag
     3841 gttattgcgg gaaaaaaaca cccgcaactg agctgtcatt cgccggaaaa aagaagagat
     3901 gcccggttca gggtacaaaa taaacgttga cataaagact cctgtaaaaa aacgggagcc
     3961 ttcgccccga cggacaaaaa ctcccgtcag ggttaaaaca ccaccagtac agcatggtgt
     4021 tgctgtcaga aaaagcccgt taataaaggg cgcagttata aa
//
LOCUS       DAZGHF010000249         4027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGHF010000249 DAZGHF010000000
VERSION     DAZGHF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.251"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1854
                     /locus_tag="ACHK3A_004914"
     CDS             <1..1854
                     /locus_tag="ACHK3A_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO8742856.1"
                     /translation="GGLRKTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAW
                     FWRVLWRFRPYYGQVILASFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIA
                     AILFECAAKLIRSYLTDIAGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYE
                     SVREFMTSATLLAFVDLPFILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSR
                     HINASMRESSQRQGLLVEALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSN
                     VIICFSTAVQQLNTVLLVILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIAS
                     LATRFQQARLAMQGVNDIVRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDAL
                     CGLSLCIRPGERVGIIGSIGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRD
                     KVFLLEQNPRLFLGTLRENLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQT
                     GEGGQGLSGGQRQMVALTRMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTM
                     VVVTHRLQVLNIVDRIVVMNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            1914..3161
                     /locus_tag="ACHK3A_004915"
     CDS             1914..3161
                     /locus_tag="ACHK3A_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO8742857.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            3447..>4027
                     /locus_tag="ACHK3A_004916"
     CDS             3447..>4027
                     /locus_tag="ACHK3A_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742858.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLAEGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRATEGE
                     QKPVQPEGGQEAGKTEVSSTGSTEAGSGSSSGQV"
BASE COUNT         1017 a          830 c         1197 g          983 t
ORIGIN      
        1 gggggactcc gtaagacact ttctcacgat gaattaatgg ctgtttattc aggttactgc
       61 tggtttgttc agccacaggc aacacaggat cgccgttcgg aactgccgga gtatgacatg
      121 accggggcat ggttctggcg ggtactgtgg cggttcaggc catattacgg acaggtcatt
      181 cttgcgagtt ttatcattaa ctttcttgcc ctggtcagtt ccctgtatgt catgaacgtt
      241 tatgaccggg tcatacccaa tcgctcctgg gaaacgttat ggatgctgag tatcggggtg
      301 attgcagcca ttctgtttga gtgtgctgca aagttaatcc ggagctatct gacggatatt
      361 gcgggtaaga aggcagacct gattatcagc tcagcactgt taaggcgggt gatggcagtg
      421 aaaatgtcgg acaggccggc gtcttccggt tcatacgcca gcaatttgcg ggaatatgag
      481 tctgtcaggg agtttatgac cagcgccacg ctgctggctt ttgtggattt gccgtttatc
      541 ctgcttttca tcggtgtcat cgggcttgtg gggggctggc ttgctgttgt tcctcttaca
      601 ctggttccgc tggtgattct ggcaggaata ttactgcagc gaccgctttc gcgtcatatt
      661 aatgcctcca tgcgggaatc atcgcagcgc cagggattac ttgtggaggc actggacgga
      721 atagaaaccc tgaagctgaa taatgcagtt aactgggtcc agcgccgctg ggatggatat
      781 acagccagtg tcgcccgctc gtccatacgg gtaaaaaata ccagtaatgt cataatctgc
      841 ttttccacag cagttcagca actgaatact gttctgctgg tcattctggg gacgtacctt
      901 attcacagtg atgacccaca taaccgaatc accatggggg cactgattgc cgcagttatt
      961 ctttccggtc gtgcgctggc accgcttggt cagatagcat cactggccac ccgttttcag
     1021 caggcccgcc tggccatgca gggggtgaat gacattgtcc gccgtccggt ggagcgggaa
     1081 agtcagacac attatctgac agtgaaggat atccagggag agtttcgttt cagtgatgtc
     1141 tccttttctt ataagcccgg attacccgac gcattgtgcg gtctgtcgct ctgtatccgg
     1201 cccggagagc gggtgggtat tattggctca ataggttgtg gtaaatcgac gctgctgaaa
     1261 atgatggtgg ggctgtaccg tcagaacagg ggacaaatta ctcttgacgg ggttgcgatg
     1321 cagcaactgg acccctgttt tgtccgggat aaagtgtttc ttctggaaca gaatccccgg
     1381 ctttttctgg gaactctgcg tgaaaatctg cagctttcgc gtcttccggg aagcctgtcg
     1441 gatgtggata tgattaatgc agccggacgt ttcgggtttg accgttttat ccggcatcat
     1501 ccccgcggac ttgatatgca gacaggggaa ggcggtcagg gactgtcagg aggacagagg
     1561 cagatggttg ccctgacgag aatggtactg ggttctcccc gggtggtact gcttgatgag
     1621 cccacgacag gtctggacct gcagactgag tctgttgttc tgaaaggtct gagccactgg
     1681 agccgtggac gcacaatggt ggtggtgaca catcgtctgc aggttctgaa tattgttgac
     1741 cgtattgtcg tgatgaatga agggcgggta atgatggacg gtccccgtga tgaaatactg
     1801 aaaaaactgt cagggaaaca gcagagtggg gagagcagag cgataacttc atgagcgtca
     1861 accggataag aaaaaagagc agatatgttt aacagtttgt ggaccgcagg gatatgacag
     1921 aaaaaacgga acggaataaa gcgtcgtcag aggtggtcag gcagtcagac ctggcgctgt
     1981 tgacagatgt acaggcagcc cttcagcagg aaaagcatca tgcccttttc ctgatggtga
     2041 tttttctgct ggtactggta gtgacgtttg ttatctgggc atggaacagt cctctggatg
     2101 aagtgacccg aggtcagggg agtattattc ccggcagcag ggagcaggtt atacagaccc
     2161 ttgacccggg tatactgaaa tcacttgagg tcagggaagg cgatattgtt gagaaaggtc
     2221 aggtactgct gacactggat gatacccgga gctctgccat gctgagggaa agtgaagccc
     2281 gggtgaataa cctggaggcc gtcagggcaa gactccgtgc agaagcttat tccgaatcac
     2341 tgacatttcc ggataacgtt cctgaggatt tgcgggagcg ggaaagtacg gtctattttc
     2401 tccggaagac ggagctggcg cagagcattg ccggtctgaa gcagagtaag gcgctgctgg
     2461 acagggaaat agcaatgacg cggccaattg tgcgtgaagg ggcaatgtcg gaggtcgaac
     2521 tgttacgtat gcagcgtcag tcagcggagc tgcagttaca gatggatgag aaacagaata
     2581 aatacctgac ggaagccggt gctgaactgg tgaagacaga agcagagctt gctcaggcaa
     2641 aagagaatat ggctggcaga gccgacccgg ttgagcgttc acgtctcagg gcccccctga
     2701 gaggtatcgt aaaaaatata cgggtaaaca cactgggagg ggtggtttct gcaggacagg
     2761 acataatgga aattattccg ctggaagacc agttgctgat tgaagcttat ataaatccac
     2821 gggatgttgc ctatgtcaga acagggatgc cggcacttgt aaaactgacg gcttatgact
     2881 atgccattta tggtggtctt gacggcgtgg tgacactggt aagtccggat actttacgtg
     2941 accagaaacg gccgggagat ttaaaactgg acccgaatga agcttattac cgggtactgg
     3001 ttaccaccag taataattat ctgacagaca ggaacggaaa aagattgcca gttattcctg
     3061 gcatgatagc cagcgtggat ataaaaaccg ggcgtaaaac ggtatttcag tatctgatta
     3121 aaccgataac cagaatgaag caggcacttc aggaaagata ataaaaaggt cttccgggga
     3181 aagtatttta aatcaatttt aaacgggcag aatgatttca ctttattctg cccgggtcat
     3241 tgcgcatgcc tgtatgtgat ttggggctga agataatata aatgttatat ctgtcagggg
     3301 aaaataaatc tgaaaaaagc ggaatgatta aggaggtccg gtcagagaaa taaattatct
     3361 gtaatctgat tcgtttttat ttaatgactc cctttagcgg ataaaacata agagcatact
     3421 ggttttatta acctgaggat acttatatga aaatgatttt caccggcaag gtcagtggag
     3481 aaaaaacagt actgacagcc ggagcccgtc atacagtaaa ggcacaggcc ggggaacagt
     3541 atggactggt tgatgaggtg acaggtctgg tcccggatgg tgtggaagcg gaccgttctg
     3601 gtgatgattt aattctgcgt aaaaaggaag atgatacaga aatccggatt gagggattct
     3661 gggaagaatg ccagccgggt gaaacacagt gtacagccgt gtttaatgtg gtgggtgaaa
     3721 atggccaggt tactgaagct gtcctgaccc aggatggtcc tgtactggag aatttcactg
     3781 cagggcaaag tggcacactg gctgaaggta tcaataacgg gattatctgg ggtgggattg
     3841 cttttggtgc agggctggcg gctattgcac tggctgccgg aggcggtggt ggaagcagtc
     3901 gtcataagcg tgcaactgaa ggtgagcaga agccggttca gcctgaaggc ggtcaggagg
     3961 ctggaaaaac agaagtatca tccacaggct caactgaggc gggaagtgga agctcttccg
     4021 gccaggt
//
LOCUS       DAZGHF010000250         4000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGHF010000250 DAZGHF010000000
VERSION     DAZGHF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.252"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            110..718
                     /locus_tag="ACHK3A_004917"
     CDS             110..718
                     /locus_tag="ACHK3A_004917"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO8742859.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            816..2207
                     /gene="selA"
                     /locus_tag="ACHK3A_004918"
     CDS             816..2207
                     /gene="selA"
                     /locus_tag="ACHK3A_004918"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO8742860.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2204..>4000
                     /gene="selB"
                     /locus_tag="ACHK3A_004919"
     CDS             2204..>4000
                     /gene="selB"
                     /locus_tag="ACHK3A_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO8742861.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRR"
BASE COUNT          888 a         1022 c         1193 g          897 t
ORIGIN      
        1 accatgataa atatgtgatt tattgggaag taacaaacca tcaacatttt ctgccctttt
       61 tcctatcacc aggccatact gatatttttc ctcgcacaaa ggactcggca tgaaactcgt
      121 cggtagctac accagcccgt ttgtacgcaa actttctatt ctgttgttag aaaagggcat
      181 aactttcgaa tttattaatg aactgcccta taacgcggat aacggcgtgg cgcaatttaa
      241 cccgttaggg aaagtgccgg tgctggtgac cgaagagggc gaatgctggt ttgattcgcc
      301 gatcatcgct gaatatattg aattaatgaa tgtcgctccg gcgatgttgc cgcgcgatcc
      361 gctggagtcg ttgcgggtgc gcaaaattga ggcactggcg gatggcatta tggatgccgg
      421 gctggtatcg gtgcgtgaac aggcgcgtcc agcggcgcag cagtctgaag atgaattgtt
      481 acgccagcgc gagaaaatca accgcagtct ggatgtgctg gaaggatatc tggtcgatgg
      541 cacgctcaaa accgatacgg tcaatctggc gactatcgcc attgcctgtg ccgtcggata
      601 tctcaatttc cgccgcgttg cgccaggctg gtgtgtcgac cgcccacact tagtcaaact
      661 ggtcgaaaac ctgtttagcc gcgaaagttt tgctcgcacc gaaccgccaa aggcttgatg
      721 cgcgatatgt cctcctgacc catctcacgt tacaatccgt ggttatgtta aacgcccttc
      781 tccgtgtgag agggccttga tcagccaggt ttcctatgac aaccgaaacg cgttccctct
      841 atagtcaact tccggctatt gatcgcttat tgcgcgatag ctccttcctt tctttgcgtg
      901 atacttatgg tcacacccgc gtggtggaat tgctgcgtca gatgcttgac gaagcgcgag
      961 aagtgattcg tgatagccag acgctgcccg catggtgtga aaactgggcg caagaagtcg
     1021 atgcccggtt gacgaaagag gcgcagagcg cgctgcgtcc ggtgatcaac ctgacgggaa
     1081 ccgtgctgca taccaatctt gggcgagctt tacaggcaga agccgcggtg gaagccgttg
     1141 cgcaggctat gcgttcgcca gtgaccctcg agtacgatct ggacgacgcc ggacgcggac
     1201 atcgcgatcg ggcgctggcg cagctgctgt gccgcattac gggagcggaa gatgcctgta
     1261 tcgttaataa caatgcggcg gcggtgttat tgatgttggc ggccactgcc agcgggaaag
     1321 aggtggtggt atctcgcggc gaactggtgg agattggcgg cgcgtttcgt attcctgatg
     1381 tcatgcgtca ggcaggctgc accctacacg aagtggggac caccaaccgc acgcacgcta
     1441 atgattatcg tcaggcggtg aatgaaaata ccgcgctgtt gatgaaagta cataccagta
     1501 actacagcat tcagggattc accaaagcga tagatgaagc ggaactggtg gcgctcggca
     1561 aagagctgga tgttcccgta gtgactgatt taggcagtgg ctcgctggtt gatcttagcc
     1621 agtacggttt gccgaaagag ccaatgccgc aggagttgat tgcggcgggc gtcagtctgg
     1681 tgagtttctc cggtgacaag ttgttaggcg ggccgcaggc aggaattatt gttggtaaaa
     1741 aagagatgat cgcccgcctg caaagccacc cgctgaagcg tgcattacgc gcggataaaa
     1801 tgaccctcgc ggcgctggaa gccacgttgc gtctttattt acaccctgaa gctctgagtg
     1861 aaaaattacc gaccctgcgc ctgcttaccc gcagcgcaga ggtcattcaa atccaggcac
     1921 aacgtttaca ggctcccctt gccgcacatt acggcgcgga gtttgcagtc caggttatgc
     1981 catgtctttc gcagattggc agtggttcac tgccggttga tcgcctgccg agcgcggcat
     2041 taacgtttac accccatgat ggacgcggta gccaccttga gtcattagcc gcccgctggc
     2101 gtgaattgcc agtgccggtg attggtcgta tttatgacgg acgattgtgg ctggatttac
     2161 gctgccttga agatgagcaa cggtttttgg agatgttgtt gaaatgatta ttgcgactgc
     2221 cggacacgtt gaccacggca aaacaacctt attgcaggcg attactggcg taaatgccga
     2281 tcgtctgccg gaagaaaaaa agcgcggcat gactatcgat ctcggctatg cctactggcc
     2341 gcagccggat ggccgcgtgc ctggttttat cgacgttccc ggtcatgaaa agtttctttc
     2401 caacatgctg gcgggcgtcg gtggtatcga tcacgcgctg ttggtggtgg cgtgcgatga
     2461 cggtgtgatg gcacagaccc gtgagcatct ggcgattttg cagctgactg gtaacccgat
     2521 gctgacagtg gcgctgacca aagccgatcg cgtggacgaa gcgcgtgttg atgaggttga
     2581 acgccaggta aaggaggtgc tgcgggaata cggttttgct gaggcaaaac tgtttatcac
     2641 cgcagcaacc gaaggtcggg gaattgatgc cctgcgcgag catctgcttc agttaccaga
     2701 acgcgaacac gccagccaac atagtttccg cctcgcgatc gaccgtgcat ttaccgtaaa
     2761 aggtgccggg ctggtcgtca ccggtacggc gttaagcggg gaagtgaagg taggcgattc
     2821 actctggctg actggtgtaa ataaaccgat gcgcgtgcgt gcgctgcatg cgcaaaatca
     2881 gccaacagaa actgcccatg ccgggcagcg tatcgcgctt aacatcgcgg gtgatgcgga
     2941 aaaagagcag attaaccgtg gcgactggct gcttgccgat gcgccgccgg agccgttcac
     3001 acgggtgatt gtcgagcttc aaacccatac accgctgacc cagtggcagc cgctgcatat
     3061 tcaccacgcc gccagccacg tcacgggacg cgtttcgctg ctggaagata accttgccga
     3121 gctggtgttc gacacgccgc tgtggctggc ggataacgat cgcttagtgt tgcgcgatat
     3181 ctcggcccgc aacacgctgg cgggggcgcg tgtggtgatg cttaacccgc cgcgtcgcgg
     3241 taagcgtaag ccggaatatc tgcaatggct ggcgtctctt gcacgggcgc agagcgatgc
     3301 cgatgcgtta tctgttcatc tggaacgcgg agcggttaac cttgcggatt tcgcctgggc
     3361 acggcagctc aacggcgaag ggatgcgcga actgctgcaa cagcctggct atattcaggc
     3421 tggttatagc ttgttgaatg cgccggttgc cgctcgctgg cagcggaaaa ttctcgacac
     3481 attagcgact tatcatgagc aacatcgcga tgaacctggt cctgggcgag aacgtctgcg
     3541 acgtatggcg ttgccaatgg aagatgaagc gctggtactg ttgctgattg aaaagatgcg
     3601 cgaaagcggc gacatcctca gtcatcacgg ctggctgcat ctgccggatc acaaagcggg
     3661 cttcagcgaa gagcagcagg ccatctggca aaaagcagag ccactgtttg gtgacgaacc
     3721 gtggtgggtg cgagacctgg caaaagaaac gggaaccgac gagcaggcaa tgcgcctgac
     3781 gctacgccag gcggcgcaac aggggataat tactgcgatc gtcaaagatc gttattaccg
     3841 taacgatcgg attgtcgaat ttgccaatat gatccgcgat ctcgatcagg aatgtggttc
     3901 aacctgcgcg gcggatttcc gcgatcgctt aggcgtaggc cgaaagctgg caattcaaat
     3961 cctggaatat tttgaccgca ttggctttac gcgtcgtcgt
//
LOCUS       DAZGHF010000251         3996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGHF010000251 DAZGHF010000000
VERSION     DAZGHF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.253"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(72..818)
                     /gene="cutC"
                     /locus_tag="ACHK3A_004920"
     CDS             complement(72..818)
                     /gene="cutC"
                     /locus_tag="ACHK3A_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO8742862.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(832..1398)
                     /gene="yecM"
                     /locus_tag="ACHK3A_004921"
     CDS             complement(832..1398)
                     /gene="yecM"
                     /locus_tag="ACHK3A_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO8742863.1"
                     /translation="MANWHSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1614..3347
                     /gene="argS"
                     /locus_tag="ACHK3A_004922"
     CDS             1614..3347
                     /gene="argS"
                     /locus_tag="ACHK3A_004922"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO8742864.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3400..3792)
                     /gene="flhe"
                     /locus_tag="ACHK3A_004923"
     CDS             complement(3400..3792)
                     /gene="flhe"
                     /locus_tag="ACHK3A_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO8742865.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAIEPL
                     RFIWEVSGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(3792..>3996)
                     /locus_tag="ACHK3A_004924"
     CDS             complement(3792..>3996)
                     /locus_tag="ACHK3A_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFO8742866.1"
                     /translation="DRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQL
                     VVLSNLELSDNRHIRMTATIGGK"
BASE COUNT          999 a         1079 c         1019 g          899 t
ORIGIN      
        1 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
       61 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
      121 gccccgtcta cgacataacg cgaatactcg tccgcgtgtg catctgatga catggacaat
      181 ccttgattac gataacgcat tggtgaggct tgccacgctc ccgcggagct atggacttcc
      241 agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc tccggccata
      301 atgattggag catcaccatg ggcaataagt tccataattt ttgataaacc ttgcagtgcg
      361 tctgattttt gccctgatgt cagtacccga gcaacgccca attccgcaag attattgagt
      421 gtatttaaag ggttagcgca catatcgaag gcgcgatgaa aagtcacggc cagcggaccg
      481 gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac atcgagaaca
      541 cccgtcacca gtccaggaaa acctaattcg cggactgtgc gcacatcctc aagaatggcg
      601 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaataat aggatgcaca
      661 gggatcgtca cccgctggcg cacggatttc agcaccccca acgacggcgt taagcccccc
      721 tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc cgttagcgcg
      781 cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta attacgccga
      841 ctgctcgctg gcaacgatct cttcaatgga ccacggatga aatttaatgg tcgttttgcc
      901 atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg gcgaactggt
      961 tttaactgag atccccggca ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     1021 attcagcgtt tctggatcgc ccggcagaac gatttcaata tgttcccaac cttcgtgcgg
     1081 gtaacgtttt tccccgggcc acggtagctc cacaatggaa aactgccagt gcgcaacctg
     1141 taccggttca tgcaatttaa acagacaaat cggcctgcca ttgatcatat tttctgacaa
     1201 aagctcgccg cactgttcaa acccgcgacg ccagcgctca gcggtggcgt tttgatggca
     1261 acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc aaccccaaac ggcgggaaag
     1321 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca gctcgtcgat
     1381 agaatgccag ttcgccataa tcactcttcg tcttccacta aaagcgttaa tttaccctgt
     1441 tgccctgcgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt gaaaacgaca
     1501 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     1561 tctttaactg gtgcgggtaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     1621 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     1681 attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat caagctaacg
     1741 gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca gagcaggtgc
     1801 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgcgggt cctggcttta
     1861 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     1921 atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac tctgcgccaa
     1981 acgtggcgaa agagatgcac gttggtcacc tgcgctctac cattattggt gacgcggcag
     2041 tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg
     2101 gcactcagtt cggtatgttg attgcgtggc tggaaaagca gcagcaggaa aacgccggtg
     2161 aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat tacgacgaag
     2221 atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat
     2281 atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac cagatcacct
     2341 acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggtgaaagc ctctacaacc
     2401 caatgctgcc gggtatcgtg gcggacctga aagccaaagg tctggcagta gaaagtgaag
     2461 gcgcgaccgt cgtattcctt gatgagttca aaaacaagga aggcgaaccg atgggcgtga
     2521 tcattcagaa gaaagatggt ggctatctct acaccaccac tgatatcgcc tgtgcgaaat
     2581 atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc cgtcagcatc
     2641 aacacctgat gcaggcgtgg gctatcgtcc gtaaagcagg ctatgtaccg gaatccgtac
     2701 cgctggaaca ccacatgttc ggcatgatgc tgggcaaaga cggcaaaccg ttcaaaaccc
     2761 gcgcgggtgg tacagtgaaa ctggccgatc tgctggatga agccctggaa cgtgcacgcc
     2821 gtctggtggc agaaaagaac ccggatatgc cagccgacga gctggaaaaa ctggctaacg
     2881 cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc acggactaca
     2941 tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac atgcagtatg
     3001 catacacgcg tgtattgtcc gtgttccgta aagcagaaat tgacgaagag caactggctg
     3061 cagctccggt tatcatccgt gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt
     3121 ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg tgtgcttacc
     3181 tgtacgatct ggccggtctg ttctctggtt tctacgagca ctgcccgatc ctcagcgcag
     3241 aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacggcg aagacgctga
     3301 aactgggtct ggatacgctg ggtattgaga cagtagagcg tatgtaatcg cgttatacgg
     3361 caaaagccga tgtcatggca gacatcggct tttttatcat taacgataat tcacaatcac
     3421 ttcattccgt tgtaccttta gcggcggaat taaccgccca ccgccagaca cttcccagat
     3481 aaatcgcaac ggttctatcg ccggtatacc ggaaaaggcc acggtggttc cactctgccc
     3541 ttctaattcg acacaacgag attgtgaaca caagcgaacc cgcagtcctg caggtgtcgg
     3601 gccgataagc tgataacgcc acgccaccag cgtcatcaat cctgcagcag gttgtcgagt
     3661 agaaagaggc gctgacgaca tcgactcacc acggtgattc agcgtaacgc ccacactact
     3721 cgcctgccac atcccctccc cggcggcttg caccagcagc ggaaacaata atattgctaa
     3781 taaagttctc atcatttacc gccaattgtc gccgtcatgc ggatatgtcg gttatcagat
     3841 agttccagat tcgacagcac cactaactgc ggcaggctgc ggcgcaggaa gcgagacaat
     3901 aatggtcgca gcgcgtggtt caccaacaat accggcggcg cacccaacat ctcctgacgg
     3961 gatagcgctt cctgagtttg cgccagtaaa cgatcc
//
LOCUS       DAZGHF010000252         3941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGHF010000252 DAZGHF010000000
VERSION     DAZGHF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.254"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            428..979
                     /gene="ybcL"
                     /locus_tag="ACHK3A_004925"
     CDS             428..979
                     /gene="ybcL"
                     /locus_tag="ACHK3A_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO8742867.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            989..1786
                     /locus_tag="ACHK3A_004926"
     CDS             989..1786
                     /locus_tag="ACHK3A_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8742868.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHTLTVDNNQIILFPCGYEFYIENYPEAGLYLAEMLYLPIDLIESFQKLYTVTDQIR
                     NKTSFFLPQNPELIYCWEQLKTSVSRGFSTKIQEHLAMGVLLSLGVNHVNHLLLSYSK
                     QSLISRCYNLLLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            1903..2004
                     /locus_tag="ACHK3A_004927"
     CDS             1903..2004
                     /locus_tag="ACHK3A_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742869.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            2001..2456
                     /gene="ybcN"
                     /locus_tag="ACHK3A_004928"
     CDS             2001..2456
                     /gene="ybcN"
                     /locus_tag="ACHK3A_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO8742870.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            2456..2626
                     /locus_tag="ACHK3A_004929"
     CDS             2456..2626
                     /locus_tag="ACHK3A_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO8742871.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            2619..2909
                     /locus_tag="ACHK3A_004930"
     CDS             2619..2909
                     /locus_tag="ACHK3A_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO8742872.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            2906..3268
                     /gene="rusA"
                     /locus_tag="ACHK3A_004931"
     CDS             2906..3268
                     /gene="rusA"
                     /locus_tag="ACHK3A_004931"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO8742873.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            3265..3405
                     /locus_tag="ACHK3A_004932"
     CDS             3265..3405
                     /locus_tag="ACHK3A_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO8742874.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            3491..3874
                     /gene="quuD"
                     /locus_tag="ACHK3A_004933"
     CDS             3491..3874
                     /gene="quuD"
                     /locus_tag="ACHK3A_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO8742875.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT         1146 a          776 c          924 g         1095 t
ORIGIN      
        1 aagtgcaaga cagaattaat tatctttgac gaaacttaat gggtaattac tttgtttgct
       61 cccacaagcg agttttgtac ggctgtattg gggtagtaaa tgagctatac aatcttaatc
      121 atttgttagg tgagaactct tggtcgcaga ttcaaatact gaaaatacgt gacaaattat
      181 tatgagcaaa atggtgtatg tcacgtattt tgaatggtag gttaaaaaat aacaccgact
      241 ttcgtaggta ttactaataa taaagcagag tttttagata gtatcaatgt gctttgtgta
      301 tattgtggca aataattggg ttgggggtac aattgtgatt gcttttgcat gaacattgcg
      361 cctttatgca taatgagata aaggaatatc aaataaaata acgataggtc ataacaaaga
      421 ggtttttatg aaaacactta tcgtttcaac tgtattggca ttcataacat tttctgcgca
      481 ggctgcagca tttcaggtca ctagtaatga aataaaaaca ggagagcaac ttacaacgtc
      541 tcatgtcttt tctggatttg ggtgtgaagg tggtaataca tcgccctcat taacctggtc
      601 tggtgttcct gaaggtacca aaagctttgc cgtaactgta tatgatccag atgcacctac
      661 aggcagtggt tggtggcatt ggactgttgt taatattcca gcaacagtaa catatttgcc
      721 cgttgatgca gggagacgtg atggaacaaa actgccgact ggtgctgttc aaggccgaaa
      781 tgattttggc tatgctgggt ttggtggcgc atgtcctcct aaaggagata aaccacatca
      841 ttaccagttt aaagtatggg ctctaaaaac tgaaaagatt cctgtagatt ctaactccag
      901 cggagcgtta gttggttata tgcttaatgc taataaaatc gcaaccgctg agataacacc
      961 agtttatgag ataaagtagg gtgagagtat gctggcaaga ggtaagacta acttaaagat
     1021 cgaagaaata cggatgcata aacatcatga gattcatagg gttaagcctc ttatgccagc
     1081 tttgtgtcgt atccgtcagg gaaagaaagt tatcaattgg gagacgcata ctttaactgt
     1141 tgataataat caaataatat tatttccttg tggttatgaa ttttatattg agaattatcc
     1201 tgaagcaggg ctttatcttg cagaaatgct ttacttaccc attgatttaa ttgagagttt
     1261 ccaaaaactt tatacggtaa ctgatcaaat acgtaacaaa acaagtttct ttttacctca
     1321 gaatcctgag ttaatatatt gttgggagca actaaaaaca tctgtttccc gaggcttctc
     1381 aactaaaatt caggagcact tagcaatggg cgttctactt tcgttaggag tgaatcatgt
     1441 taatcattta cttttatcat atagtaaaca atcattgata agtcgttgtt ataacctgct
     1501 gctatccgaa cccggcacaa aatggacagc aaacaaggtt gctcgatatc tctacatttc
     1561 tgtttctaca ttacatcgcc gtctagcaag cgagggggta agtttccaaa gtatactgga
     1621 cgatgtgagg ttaaataatg cgttgtctgc tatacaaacg acggtaaaac ctataagcga
     1681 gattgccaga gaaaatggtt ataagtgtcc ttctcgtttt actgaaagat ttcataatcg
     1741 ttttaatata acaccaagag agataagaaa agcttccaga gagtaaaagt gttttaagaa
     1801 ggagcaattc tatcgatttt gattttggga aatcaacacg gcataattat gtcaccggag
     1861 cctgaacaac tccggtgact tctgcgctaa acggggacgt ttatgcgcac atacaatcca
     1921 aactctcttc tcccttcaca gatgcagaaa tgcacctgca attctttgca tctagcgttt
     1981 gacctctgcg gaggtgaagc gtgaacctct cacaagacgg catcaaatta catcgcggca
     2041 acttcaccgc tatcggtcgg cagatccagc cttatctgga ggagggcaaa tgctttcgca
     2101 tggtgcttaa accgtggcgt gagaaacgca gtctttccca gaatgcactc agccacatgt
     2161 ggtacagcga aatcagtgaa tacctcatca gcaggggtaa aacgttcgcc actccagctt
     2221 gggtaaaaga tgctctcaaa cacacatatc tcggttatga aaccaaagac ctggttgatg
     2281 tcgtaaccgg tgatatcacc actatccagt cgttacgcca tacctccgat cttgataccg
     2341 gagagatgta tgtcttcctg tgtaaggttg aagcctgggc ggtgaatatt ggctgccacc
     2401 tgactattcc gcagagctgc gagttccagc tgctccgcga caagcaggag gcgtaatggc
     2461 tacaccgctt attcgtgtca tgaacggaca catctacaga gtatcaaatc gtcgtaagcg
     2521 taagcctgag ctgaagccat ccgaaatacc aacactgctc ggatataccg ctagcctggt
     2581 tgataaaaaa tggttgcgac tggcagcaag gaggaatcat ggctgatttg agaaaagcag
     2641 cgcgtggtcg ggaatgccag gtaagaatcc ctggcgtatg taatggcaat tctgaaacgt
     2701 ctgtactggc acatatccgg ctggctggat tgtgcggtac cggtatcaaa ccgccagacc
     2761 tgattgccac cattgcatgt tctgcctgcc acgacgaaat cgaccgccgc acacattttg
     2821 tcgatgctgc atatgcaaaa gaatgcgcgc tggaaggtat ggcgagaaca caggttatct
     2881 ggctgaaaga gggggttatt aaggcgtgaa tacctacagt atcacattac cctggcctcc
     2941 gagcaataat cgctattacc gccataatcg cgggcgcacg cacgtcagtg cagaggggca
     3001 ggcataccgc gataacgtcg cccgaatcat taaaaacgca atgctggata tcggcctggc
     3061 tatgcctgtg aaaatccgca ttgagtgcca catgccggat cgccgtcgcc gtgacctgga
     3121 taatctgcaa aaagccgctt ttgacgcact caccaaagca ggtttctggc tggatgatgc
     3181 tcaggtcgtt gattaccgcg ttgtgaagat gcctgttacc aaaggtggca ggctggaact
     3241 gaccatcacc gaaatgggga atgaatgatg tttgagtttt atatggcaga acttcttcgc
     3301 caccgctggg ggcatctgcg cttatatcgt ttccccggtt ctgttttgac cgattaccga
     3361 atactgaaga attacgccaa aaccctgaca ggagcaggag tatgaagtca gagataacaa
     3421 tcaactaata ctgttttgtt gatttttgct tgtaattggc gttctggtct gatttttgtg
     3481 gagtaagttg atgcgtgata ttcagatggt tcttgagcgt tggggagcgt gggcggctaa
     3541 taatcatgaa gatgtgacct ggtcgtccat tgccgccggt tttaagggat taattacttc
     3601 aaaagtaaaa tctcgcccgc aatgttgtga cgatgacgcg atgattattt gcgggtgcat
     3661 ggcccgtctg aaaaagaaca acagcgattt gcacgattta ttagtagatt attatgtagt
     3721 cggtatgaca ttcatgtcac tggcaggtaa gcattgctgc tctgatggtt atatcgggaa
     3781 aaggttacag aaggctgagg gcataattga agggatgtta atggcattag atatccggtt
     3841 agagatggat atcgttgtta ataactctaa ttaatatgcc aattgtttac taaaaattat
     3901 taaaaatggg gcgttgcaac gcccccaaaa ataaagggta a
//
LOCUS       DAZGHF010000253         3857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGHF010000253 DAZGHF010000000
VERSION     DAZGHF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.255"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..639)
                     /locus_tag="ACHK3A_004934"
     CDS             complement(<1..639)
                     /locus_tag="ACHK3A_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO8742876.1"
                     /translation="MIRKSCIAACLSITLCSLSYAGGVPTVSIAELTQLVVNAQQQAQ
                     EALAQLNKAKEAIRQAKDQYDHYKSILQGNDKLGAFLNDPYINKLLPMKDWQDIYNQT
                     QDLTDLRKRYGITGYEPDVQKLFDKLLAQVDVYEKQYDATVTRVNHAEELRNKLNSVQ
                     TPKDREQLALRYQQEMLELENQRMQLENTRYLMEQKERIENKKNEEDFYNFMW"
     gene            complement(709..3243)
                     /locus_tag="ACHK3A_004935"
     CDS             complement(709..3243)
                     /locus_tag="ACHK3A_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFO8742877.1"
                     /translation="MQDALFKNAFSPESNAKVPWLGHHVAPEICTLGTSHILSLIRFS
                     GVPHDTRDQAMLNKEFMYLARCFKALGKQEGNNLKLQTYTFKRRIHLTHQYNLELPVL
                     QDLVDCWVKPFRNGTFRQVGYSMALILKFRDLDDGINRMRELLSLCREMLGDFGVSVM
                     ELEERDGKLYSQIGRFYSFILNGEEKDIPVTDTRLGDSIIDSETGFGAYDYVENRPSY
                     GKRRFASTYDLRDYPESKSYPGMWDEVLDEQYDFCMVQSFLFEDRNRMKRRLKTQMSD
                     LSSTEGQSSETEELADSVQEVTQGKKVFGEYYASLIVFGDTPEEAVDNGARMQSLLMA
                     RDTGFVRSRSTNYVTWLTLFPGYADVIYSMPKSTENLACGFSLHATPCGKADGNPPGD
                     GTALMPVMTENGGMYFLNAHASPLGKNCRGEKYPGHKTTIAMTGAGKTTVESLELVFF
                     SRWNPMIFSLDYNNSLENVLRALGTRYFNIKPGEFTWIQPFQMEDSVELRQYLFDVLV
                     QCAGGADSTEESKIQMSIDAVLKHKNVESRSFSLLLQSIPDTGPGGLRERLSKWCRDD
                     GNGRRGQYYWILDSPRNDFDPTLFRRLGFDCTKILNMEFATRHPQAMEVLLNTFFFLK
                     RRMHENEPGSLLINSIAEFWAPLMFESTANSIMEILHAGRTRGEMVMMDTQVPEYVLN
                     NERGPSVIQQTITQEWMANEKATSDSYSKFSVTSKEFSKIEEFSAYSRKVLVKQGGAS
                     VVLKVDMQDKLKYWLPLLSSDYANIPVAKRVREALGTDDPAVWVAPFLDEMVAIGVRK
                     ELAEKYPEQDVNDPQVWEPAFIEVMKQLERPVSMKLTTINNINSKS"
     gene            complement(3200..3571)
                     /locus_tag="ACHK3A_004936"
     CDS             complement(3200..3571)
                     /locus_tag="ACHK3A_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFO8742878.1"
                     /translation="MGETNEILEFDTNNAMNRVAMFAGIPVFALLFLVVIGCILTFSS
                     VWMWGWWGFIVTIPFILAIVGLRIICERDDKAFRRFRFQLRRWKMNRKYGRHLLLTSR
                     NPRWRDKYARRVIQKRILTGK"
     gene            complement(3571..>3857)
                     /locus_tag="ACHK3A_004937"
     CDS             complement(3571..>3857)
                     /locus_tag="ACHK3A_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742879.1"
                     /translation="ALLFVFIPVYAHAAWYDPIVKFVKEFQTGIIIVGGAVAVGSLVY
                     VGVCWIISRMSGNMETTAMDYVKHIGVIGAVGGAVAAATWAYQLWGGTIS"
BASE COUNT         1155 a          948 c          644 g         1110 t
ORIGIN      
        1 ccacataaaa ttataaaaat cctcctcatt ctttttattt tcaattcgtt ctttttgctc
       61 cataagatag cgcgtatttt ccagttgcat tctctgattt tcaagctcta acatttcctg
      121 ttgatatctt aatgctaact gctccctatc ctttggagtc tgaactgaat ttagtttatt
      181 tctcaactct tcagcatggt ttactcgtgt gactgttgca tcatattgtt tttcatatac
      241 atcaacttga gcaagtaact tatcaaacaa tttttgaaca tcaggttcat acccggtaat
      301 accataacgc tttcttaaat cagttaaatc ttgggtttga ttatatatat cctgccaatc
      361 tttcattggc agtaatttat ttatatatgg atcattcaga aatgccccca atttatcgtt
      421 tccctgaaga atcgatttat aatgatcgta ctgatctttt gcctgtcgaa ttgcctcctt
      481 tgctttatta agctgcgcca acgcttcctg cgcctgttgc tgtgcattta caactaattg
      541 cgttaactct gcaatgctta ccgttggcac tccacctgca taacttagag aacataacgt
      601 tatagagaga cacgcggcta tacatgattt acgtatcata tagaactcca gtgtttatca
      661 ggaacgaacg ttttcgttaa acatcaaagc attcctgata ccataaaatc aagactttga
      721 gtttatatta ttgatggttg tcaacttcat agaaactgga cgttccagtt gcttcatcac
      781 ttcaataaat gcaggctccc atacttgagg atcattgaca tcttgttcag gatacttttc
      841 cgcaagctct ttccttactc caatagccac catctcatcc agaaatgggg ccacccatac
      901 agccggatca tctgtaccta acgcttccct aactcgttta gctacaggta tattggcata
      961 gtcagatgac aataaaggca accaatattt cagcttatcc tgcatatcca ccttcagaac
     1021 gacagatgca cctccctgtt ttaccagaac cttacgtgaa taagccgaaa actcctctat
     1081 tttactgaac tccttagaag ttacagaaaa tttactgtaa ctgtctgagg tagctttttc
     1141 atttgccatc cattcctgcg taattgtctg ctgaataact gaaggaccac gctcgttatt
     1201 cagaacatat tcaggaacct gagtatccat cataaccatc tcaccacggg tcctccctgc
     1261 atgtaaaatt tccataatgg aattagctgt actctcaaac attagaggtg cccaaaattc
     1321 cgctatactg tttattagta agctgcctgg ttcattctca tgcattcttc tcttcaggaa
     1381 aaagaaggta tttaataata cttccattgc ctgaggatga cgtgtagcaa actccatgtt
     1441 aagaattttt gtacaatcaa atccaagacg gcgaaacaac gtagggtcga aatcatttct
     1501 gggactgtcc aagatccagt aatactgtcc acgtcttcca ttcccatcat cacgacacca
     1561 ctttgataat cgttccctga gtccgccagg accggtatca ggtatagact gtaataacag
     1621 tgaaaaacta cgactttcta catttttatg cttcagaaca gcatcaatcg acatctgtat
     1681 tttagattct tcagtgctgt cagcacctcc ggcacactga acaagaacat caaacagata
     1741 ttgacgtagt tcaaccgaat cttccatttg aaacggttgt atccaggtaa attcaccagg
     1801 ttttatatta aaataacggg ttccaagagc acggagtacg ttctccagcg aattattgta
     1861 atccagagaa aatatcatag gattccagcg actaaaaaac actaattcaa ggctctcaac
     1921 cgttgtttta cctgccccag tcatagcaat tgtcgtttta tgccctggat acttctcacc
     1981 tctacagttc ttccctaaag gactggcgtg tgcattcaga aaatacattc ccccgttttc
     2041 cgtcatcact ggcatcaacg ctgttccgtc tccaggagga ttcccatcgg ctttcccaca
     2101 cggagtcgcg tgtagggaaa atccacaggc caagttttct gtagattttg gcatcgaata
     2161 aatcacatcc gcataaccag gaaatagcgt cagccaggtt acataatttg tacttcggga
     2221 acgaacaaag ccggtatcgc gcgccattaa caacgattgc attcttgctc cgttatcaac
     2281 agcttcttca ggtgtatctc caaatacaat caggctcgca taatattcac caaaaacttt
     2341 tttcccctgg gtaacttcct gaacactatc agcaagttct tcagtttcac tggattgtcc
     2401 ttccgtagac gataaatccg acatctgagt tttcaggcga cgtttcattc ggttacgatc
     2461 ttcaaaaaga aaagattgaa ccatacaaaa gtcatactgt tcatccagca cttcatccca
     2521 cattcctggg taggatttgc tttctggata atcccttaga tcataagtag aagcaaatcg
     2581 gcgctttcca tacgacgggc ggttttccac ataatcgtaa gcgccaaatc ctgtttcact
     2641 gtcaataatc gagtctccca ggcgggtgtc agtaacagga atatctttct cttcaccgtt
     2701 cagaataaac gagtaaaagc ggccaatctg cgaatacagt tttccgtccc gctcctccag
     2761 ctccataaca gaaacaccaa aatcccccag catctctcta cataaagata acaattcccg
     2821 cattcggtta ataccatcgt ccagatcccg aaacttaagg ataagggcca tagaatatcc
     2881 aacttgccgg aatgtcccat tacgaaatgg ttttacccag caatcaacca aatcctgaag
     2941 aacaggtaat tcaagattat actgatgtgt gagatgtata cgacgcttaa aagtgtacgt
     3001 ctgcaatttc aggttattcc cttcctgttt tcccaaagct ttaaaacatc tggcaagata
     3061 cataaactct ttatttagca tagcctgatc tcgcgtatca tgaggaacac cagaaaaacg
     3121 gatcaaactg agaatatgag acgtgcccag agtacaaatt tcaggtgcaa catggtgccc
     3181 caaccagggc accttagcat tactttccgg tgagaatgcg tttttgaata acgcgtcttg
     3241 catatttatc tctccatcgg ggatttctgg aagtcaacag taagtgacga ccatatttac
     3301 gattcatttt ccagcggcgt aattgaaaac gaaaacgacg aaacgctttg tcatcacgtt
     3361 cacaaataat acgcaatcct acgattgcca gaataaaagg gatggtgaca ataaatcccc
     3421 accatcccca catccataca gaagaaaatg tcaaaataca gccaataaca accaaaaaaa
     3481 gcaatgcaaa aacagggatc cctgcaaaca tagccaccct gttcatcgcg ttattagtgt
     3541 caaactcgag aatttcgttt gtctctccca tcagctaatt gttcctcccc acaactggta
     3601 cgcccaggta gctgcggcaa cagcgccacc aacagcacca ataacaccaa tatgtttcac
     3661 ataatccatt gccgttgttt ccatattccc ggacatacga gaaataatcc aacagactcc
     3721 cacgtagaca agagaaccaa cagcaacagc gcccccgaca ataataattc ctgtctgaaa
     3781 ctccttaaca aatttaacta ttggatcata ccatgctgca tgagcatata caggaataaa
     3841 aacaaataat aacgcaa
//
LOCUS       DAZGHF010000254         3742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGHF010000254 DAZGHF010000000
VERSION     DAZGHF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.256"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            286..516
                     /locus_tag="ACHK3A_004938"
     CDS             286..516
                     /locus_tag="ACHK3A_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFO8742880.1"
                     /translation="MHKRMTITLDEAVYEGLYRTVGKRRMSQFIEDLLRPHVVDSSLD
                     DGYKAMAEDTERETLAMEWCNALAGDMADAAR"
     gene            479..829
                     /locus_tag="ACHK3A_004939"
     CDS             479..829
                     /locus_tag="ACHK3A_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO8742881.1"
                     /translation="MHWLEIWLMRRGEAWWVEFEPAIGSEIRKTRPAVIVSNDAANRN
                     LARVVVVPLTSNTERLYPGEAVVTVGGRRSKAMADQIMAADKSRLKAHIDTLSKADML
                     LVEDAIKIHLALPR"
     gene            1611..1865
                     /locus_tag="ACHK3A_004940"
     CDS             1611..1865
                     /locus_tag="ACHK3A_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HFO8742882.1"
                     /translation="MKITTLSSRELNQDVTRAKRATNYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
     gene            1865..2281
                     /locus_tag="ACHK3A_004941"
     CDS             1865..2281
                     /locus_tag="ACHK3A_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO8742883.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCARLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
     gene            2668..2856
                     /locus_tag="ACHK3A_004942"
                     /pseudo
     CDS             2668..2856
                     /locus_tag="ACHK3A_004942"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
     gene            complement(2927..>3742)
                     /locus_tag="ACHK3A_004943"
     CDS             complement(2927..>3742)
                     /locus_tag="ACHK3A_004943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFO8742884.1"
                     /translation="GSLTKAPAKSIADILIKIISPVVVVSIVRSPADFLYLKFSVGAF
                     LFCSLLCLIAYFLSSPLFKGKERNLFAFTAGTGNTGYFALPIVLSLFNENQVAIAIFI
                     IIGVNLYEFSVGYYVMSMNGGIREAIQKTLKLPVIHAAILGIVIKYSGVHLNNDILYV
                     LDNFKGAYSVMGMIIIGMVLSVNAKSIPDFKFLLVALSWKHLLVPVASCVFIFIPHEQ
                     FLVVFLMALTPLAGNTVVFSSSLDLHPEKAASAVLLSSLLSIVLIPVSMKIIS"
BASE COUNT         1143 a          788 c          819 g          992 t
ORIGIN      
        1 ccctgacggc ccgctaacgc ggagatacgc cccgacttcg ggtaaaccct cgtcgggacc
       61 actccgaccg cgcacagaaa ctctgtcatg gctgaaagcg ggtatggctt agcaggatga
      121 ggatggttaa ggtgaaatct atcaatcagt accggcttac gccgggcttc ggcggtttta
      181 ctcctgtgcc atatgcaaca actgagtacc gccttccatg ccgctgacgc ggcatcaaaa
      241 tatgctaata tacgttatac gtattacttg tcaggaatgt gaattatgca taaacgtatg
      301 acgattacac ttgatgaagc tgtttatgag ggactttacc ggaccgtggg aaaacgccgg
      361 atgagtcagt tcattgaaga tttgttgcgt ccacatgtgg tggattcatc gcttgatgat
      421 ggttataagg ctatggctga agatacagag cgggaaacgc ttgcaatgga atggtgtaat
      481 gcactggctg gagatatggc tgatgcggcg cggtgaagca tggtgggttg aatttgagcc
      541 tgctattggc agtgaaatac gcaaaactcg tccggctgtg attgtgagta atgatgcggc
      601 caacaggaac ttagccaggg tcgttgtggt tcctctgacc agcaacactg agcgactgta
      661 tcctggtgag gcggttgtta ctgtgggagg gcgacgcagt aaagctatgg ctgaccagat
      721 tatggctgcg gataagtccc gactcaaagc gcatattgat acgctttcaa aagccgatat
      781 gctgctggtc gaggatgcga ttaaaatcca tctggcttta cccaggtagg ggttctgcgc
      841 ccatttcctc caaaagtctc actcttagca taaaaagggg tccgcttgga aaacggaaaa
      901 tatcccacgc taagactgtt ttttgtacga ttcgatatcg acactgcgta atggtcggta
      961 cttccctgta cccagtttaa gattattacg ccagacataa tccccattaa gattgatatg
     1021 ctcccagccc aacggcgaaa ggtgagagag cagttggacc cgcgggggca tttgaacaga
     1081 aacttacggt ttcaactgct gaacaaagga tgaaaccgta atttcttgaa tgtgagttta
     1141 tccggctctg gctgctgcaa tatatttata cggggttcca ggccggaaac ctggattttt
     1201 ccgcgaccag taacggcgtg ggttgtcaca taaacgaccg attgagtgac tgtcactgaa
     1261 acaacgattt ctgcatcaga tttcgtcaca taacccgtca cctattcaac gtcatgcaaa
     1321 tctgtcaatt actggctttg gttacggaat tttctaggtt tccggcttag aacccccttt
     1381 tcaaggtttt cagccgtgta acacctgccg cctaaaaaaa gccgcatggc ctccctggtt
     1441 ttgtttctca catatcatcc ctcattaatg ctaatgtatg catacattag cattaatatc
     1501 tgatagcaac aagaggcgaa gactacccct aatcaagtac atttcttgca ttgtactatt
     1561 tcatggccta caatgtagta acgtacctat atgcatcaaa aggcaacaac atgaagatta
     1621 caaccctatc cagtcgggaa cttaatcagg atgtaactcg agccaaacgc gccacgaatt
     1681 atggtcccgt atttattact gatcggggta aaaccgcaca tgtacttctt agtatcgaag
     1741 aataccgaaa attgacgcaa caacgacgaa ctctcattga catcctggcg atgcccgatg
     1801 ctgatgcagt tgaatttgaa ccgcctcgta ttacggttag tttaaaacct gcggagtttt
     1861 tatagtgtat attcttgata ctaatgttat ctctgagcta cggaaggctc gaacaggacg
     1921 tatagataat aatgttgcaa aatgggcatt gcatacagat gcggcagatt tctttatctc
     1981 tgttatttcc ataatggaac ttgaacaggg aatattacaa gttgaacgtc gagatatgcg
     2041 acaagggatt atgctacgtt cctggatgga tcagcatgtg ttaccagcgt tttctgataa
     2101 gatacttcct gtagatactg cggttgcatt acgttgtgct cgtttacatg ttcctgacag
     2161 gcgcagtgaa cgagacgcat tgatcgcagc aacggcgctg gtacatggga tgacggttgt
     2221 aaccagaaat gttggtgatt ttgaattaac cggagtatca attattaatc cctgggcttg
     2281 atatcgcttc ccttcttcta gggctccgtt tgggaaacgg aatcgtacta taagagcgta
     2341 aggtgtcagg ttgaagtcac agactccgcg tcaggagtgc ccagaaaatt cgaaatagac
     2401 cctattctaa cgtgtttcgc ccaaacaacc tgacgtcaga ctgaagtaca ctccaacctg
     2461 acagcagtat gagcaacaac aagaatggtg cagctttggc ttcgatagct gtctcttagc
     2521 gtacaaaaat atccgtttcg tgagttgacc ccctatacgg cgttttcaga ggatttgact
     2581 ttaatcagat cacctaaatg cgataccgaa agatgatgct gagcaacggg gattgccttt
     2641 tgatgcaaaa atactgaaaa agcgttaagg ctttacacca gtcgttacgc ataccagaag
     2701 gcgttgcgaa agatgaaaga acgcatagac cttgacggaa gagcagcagg agaagtcacg
     2761 ccagaagaaa aggaacaggc aaagatccat ataatgggaa tagatgaaaa acagaagcga
     2821 agggcagaag aaaaaagacg tcaacaaaca aagtagaact gttaaatata gattatggta
     2881 agcctgttct aaacacaggc ttaccattga caaatggtat tcactcctag cttattatct
     2941 tcatacttac aggaattaac acgatagata ataacgaact aagtaacaca gcagatgcag
     3001 ccttttcagg atgtaaatca agactactac taaaaacaac tgtattacca gccaatggtg
     3061 ttagcgccat caagaataca actaaaaatt gttcatgtgg aataaaaata aaaacacatg
     3121 aagccactgg tactaataaa tgtttccatg ataatgcaac taaaaggaat ttgaagtcag
     3181 gaattgattt tgcatttaca gatagaacca tccctatgat tatcattccc atcacagaat
     3241 acgcgccttt aaaattatca agcacatata gtatgtcgtt atttaaatga acacctgaat
     3301 attttattac aataccaaga atagctgcat gtataacagg tagttttaat gttttttgta
     3361 ttgcctctct aatgcctcca ttcatgctca tgacataata gccaacacta aactcataca
     3421 agttaacacc aataataata aatatagcaa tggccacttg attttcatta aatagactca
     3481 acactatagg aagtgcaaag taacctgtat ttcctgttcc cgctgtaaac gcaaacagat
     3541 ttctttcctt acctttaaat aaaggtgatg ataaaaaata agcaattaaa caaagcagtg
     3601 agcaaaacaa aaatgccccg acagagaatt ttagatataa aaaatcagcc ggagatctaa
     3661 caatactaac aacaacaaca ggcgatatta tttttattaa tatatcagca atgctttttg
     3721 ctggggcctt tgtcaaggac cc
//
LOCUS       DAZGHF010000255         3635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGHF010000255 DAZGHF010000000
VERSION     DAZGHF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.257"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..189
                     /locus_tag="ACHK3A_004944"
     CDS             <1..189
                     /locus_tag="ACHK3A_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742885.1"
                     /translation="ETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATV
                     SVLFILLCEYAIFAEPGR"
     gene            196..1692
                     /locus_tag="ACHK3A_004945"
     CDS             196..1692
                     /locus_tag="ACHK3A_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO8742886.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFM
                     QGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEVSD
                     HYWLRLSAAMKMIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAV
                     ARIAGTAGSAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLIT
                     LTFVVFAVHFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGT
                     MFLVLPAVWLGALSWAGVSVGTVMGDAINKGTSNVSKAAAKSGDLANK"
     gene            complement(1743..1982)
                     /locus_tag="ACHK3A_004946"
     CDS             complement(1743..1982)
                     /locus_tag="ACHK3A_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742887.1"
                     /translation="MNNESEKAMWRCKPVDNPWTFTSLLKKAVCAVLPDFTPAQDKPQ
                     NMFSLSNEKDTDYSVPMRPFALYDPWKHHHDNADF"
     gene            2172..2435
                     /locus_tag="ACHK3A_004947"
     CDS             2172..2435
                     /locus_tag="ACHK3A_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO8742888.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
     gene            complement(2481..2656)
                     /locus_tag="ACHK3A_004948"
                     /pseudo
     CDS             complement(2481..2656)
                     /locus_tag="ACHK3A_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            2848..3420
                     /locus_tag="ACHK3A_004949"
     CDS             2848..3420
                     /locus_tag="ACHK3A_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742889.1"
                     /translation="MLTKKMLLVCASCVALSACSAQEIAELNRQISDGAFAVTKVLNV
                     GVNQADDDLKYLSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWAAAAISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYIT
                     YASPAPGHLKDKFIEPLVKRVKDVAEGRVR"
BASE COUNT          926 a          693 c         1055 g          961 t
ORIGIN      
        1 gagacacgtt atggctggct gggatggcgt ctgtgtcttt acagtgcgat cggatggctg
       61 gggtggagga tctggcatgc gccggggttc agggcagaat accgggcttc ttttctgcgt
      121 atggccacag tcagtgttct gtttatcctg ctgtgtgaat acgccatttt tgctgagccg
      181 gggcgttgag caatcatgac cacaaataac tatctggaat attttctgac tctgctgggc
      241 tgggtggtga ataacggtct gtggaatgtg ttgatcagca caggactgtt tgccctgcca
      301 ctggcattca ggatcatcgg gatctggctg aaagtcaggg aagaaggtgc ggatgaggga
      361 aacaaaggga tgctttccct gccgcgtatt gagcatgttc tctatggtgc tttttttgtg
      421 atgatatcct gctgtgttcc tctggtacag gtgacgctgg atacgctgtc attcgataaa
      481 tccagggcac agacctgtgg ggtatggaca cctgaagagc ccggagagac ggggtacagt
      541 ggggtgatca gcagtatagg ggatcagact gctgctgccc cggtatggtg ggtggtggtt
      601 cataaattgt cgaaaggtgt cacccaggcg gcagtggctt cgattccgtg ccggcctgat
      661 ttgcgccagt tgcgttttga gatccagcga acgtttatag aaaacagggc acttgctgat
      721 gagctgcagg actttacgaa cgactgttat tcactggcac tgtatatgtg gaaacagcgt
      781 gatcaggggc agagtaaaga taaaaaagtg cttgatgata tctcctggat agggagcgcc
      841 acattcatgc agggggatta cgggacgctg cagtcaaaga tgccgcgggc tgcgttcccg
      901 tggaatacag cacgtgacag cggacgaccg gatacggggc ggggaggcta tccgacatgc
      961 cgggagtggt ggagtgacag tagtgtgggg ctgaaaaaac gggtcatgga ggaggtcagt
     1021 gatcattact ggttacgtct gtcagctgca atgaagatga ttggcagtag tcaggtggat
     1081 tataaggagg aggtgatcaa gcgtctggtg agtccggcaa acctgacgct ctctcagggg
     1141 ggagaggtgt atcgtggcta tggtggcaat gcagatatga cgaccatgaa tgcggtggcc
     1201 cggattgcgg gaacggcggg cagtgcggta gcagcactgg gtgcgtttcc ggcactggat
     1261 gcaatgcggc aggcactgcc gatggtacag tcgataatac tgatggcgat ttacatcatg
     1321 ataccgctga ttctggcgtt tgcggcatat gaactgtcga cgttaatcac actgacgttt
     1381 gtggtgtttg cggtacactt tctgacattc tggtgggagc ttgcacggtg gcttgacagc
     1441 tggatgctga cggcgctgta cagttcggat acacataccc ggttcaatat gatgggattc
     1501 cagaacacat cggatgatct gataatgaat ctggtgatgg ggacgatgtt tctcgtcttg
     1561 ccggcagtgt ggctgggagc gttgtcctgg gctggggtaa gtgtcggaac ggttatgggg
     1621 gatgcaatta acaaaggaac aagtaatgta tctaaggcag cagcaaaaag tggagattta
     1681 gctaataagt aaaattctta gggtttcctt agctgggata attggggaaa cccttgcttt
     1741 tcttagaaat cagcattgtc atggtgatgt ttccacgggt catataatgc aaagggtctc
     1801 attggtactg aataatcagt atctttttca ttacttagtg aaaacatatt ttggggttta
     1861 tcttgagcag gtgtgaaatc tggtaaaacg gcacagactg cttttttcag cagggaagtg
     1921 aaggtccatg gattatccac aggcttacaa cgccacattg ctttttcaga ttcgttattc
     1981 atatctatac cctcagcagc aggataaata acacagtgtt atcatacgtc ttgtatgata
     2041 aatgtgcaat cacatgtctt ccatttgatg agaggctgcg atgccacatt acccttgggc
     2101 tcggggattc tgttgaaatg atctagctga gaatgtatct tattgttatg ttataacatg
     2161 agtgaagaga gatgaagcca aatatccatc ctgaataccg cacagtagtg ttccatgaca
     2221 cgagtgtgga tgagtacttt aaaataggtt ctaccatcaa aacagatcgc gagattgaac
     2281 tggatggtgt gacgtatccg tatgtgacga ttgacgtttc ctccaaatcg catccgttct
     2341 ataccggtaa actcagaacg gtggcatcag aaggcaatgt tgcccgcttt actcagcgtt
     2401 ttggtcgttt cgttgggact cagaagggag aatgatgaga gtgagagtct taacaggctc
     2461 catgcgtggt ggatttgctc ctatatttcc agacatctgg tatcacttaa cccattactg
     2521 cccgccgccg cagatattcc cgtggagagc gataacccag cgcactatgt ggatgccatt
     2581 cgttataatg ctcgaacgct tctgcaaggt tctttgctgc cgttaacccg tctggtttgg
     2641 gcatgatact gatgtagatt gaagaaggga ttgtgagttt ttccttcggc ttttaacgta
     2701 acttcctgtt ttcccgatat tccacagcaa gattaatttt tagcggagct gaatgaataa
     2761 aatcttcatc tggtgttttt gttgcgcgta aatgcaggtg taactgtgga tgttatccac
     2821 tgaagagctg ctgatgagga tgaagacatg ctgactaaaa aaatgttact cgtttgtgct
     2881 tcgtgtgttg cgctgagtgc ctgttctgca caggaaattg cagagctgaa caggcagata
     2941 agtgacggtg catttgctgt gacaaaagta ctgaacgtag gtgtaaacca ggcagatgat
     3001 gatctcaaat atctgtctca ggcaccccgg gcaaaaagag agaaacgtga tcatgtcata
     3061 gaagtccctg ttgatgtgga tacagctgcg ggaagactga aacggtacta taagttttat
     3121 tcaaatgaag aagtcgatgc gattcgtaat aacggacatg cgaacgggaa gtgggccgca
     3181 gcagcaataa gtcagaacgg gcatatgtgg gatgccatgc ccggtagtta ttataaaatg
     3241 ggcagtgact gggatggtgg cgagtttgat gatcatctga cgcttgaact tgaaaaaaat
     3301 ggcaaaggaa gtcggatata tataacgtat gcatctccgg caccggggca tctgaaagat
     3361 aaatttattg agccattagt taaacgtgtg aaggatgtgg ctgaaggccg ggtcagataa
     3421 aattaacccg ctggcattgt cgttgtgatg ggctcctctg gaggggcgat tatgctcaga
     3481 ggggctgaaa gtaagggacg cagagggcga cgaccagcga cgtctggaag agggcgtgat
     3541 atgtaaggaa gcacaccctg acggcatatc ataaaaaaac cagatatgcg ccagttaaaa
     3601 gtataaataa agagctgata aaattaacat atttt
//
LOCUS       DAZGHF010000256         3540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGHF010000256 DAZGHF010000000
VERSION     DAZGHF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.258"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..389
                     /locus_tag="ACHK3A_004950"
     CDS             <1..389
                     /locus_tag="ACHK3A_004950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742890.1"
                     /translation="LKLCFLMLLLSGCNDKAKDFIGGWTQESNEEYPVYITINYDSGI
                     YHVDVKKLDTYLVEKKKADAFDDFMWGKKSKNESENLTFDDCYKVFNFEAKAISSSVL
                     EGKGISVRMDGDRLIFDGKRFVRKKS"
     gene            415..1362
                     /locus_tag="ACHK3A_004951"
     CDS             415..1362
                     /locus_tag="ACHK3A_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO8742891.1"
                     /translation="MQVNVAETLFRTIDTATQSQMANMNTVMSVMGIVIGGIWMTYIL
                     MKSLYWYFEGLTTVIQDAFFTIFKAFAIMMASLTISWYTSTVIPPVLEFPLWLANTIA
                     GVNNDSSNLVDMVINSYISGLENLVDKMKFGFSVSSIKSMVTVGVAVVLYLLSGLPFL
                     GVAIATLLTLKVATNLILIIGPMFIACLAFPKVAHWFWGWVGVLGGFVLTQALFAITL
                     GMAISFINSNMINNGDFNISMVDIICMSLYFSAFTLLATEIPNYAASIMGGAPSGGVG
                     SMSRLLGKATGFGAASHMSKALGSKLVDRFHGRGKGRIS"
     gene            1579..2442
                     /locus_tag="ACHK3A_004952"
     CDS             1579..2442
                     /locus_tag="ACHK3A_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFO8742892.1"
                     /translation="MLFKSNKIKKETEQERLLHYGDGKLSGKSDEIGREIRAYTMAAR
                     WFEKRVAEDYRKKARNSRILAGLFGVIAIVAVIAVANLAPLKTVEPFVIRVDRNSGYM
                     DVVRPGWSKEDTKDISDDKHFISLYILSRETYNWASQKSNFAIVRQLSYSDVFSEYRN
                     FQLSGKGYVSTLNQSRQISVDIDSIVPLPVSTDKKLGERDDIKTYQVRFRQSLLDAEG
                     RPVPETGQILKLDENGKPIVKPKVVFWTAIISFDYLNTADTEGEAWLNPKGFGVLAYT
                     KTEEIRNEENN"
     gene            2426..3238
                     /locus_tag="ACHK3A_004953"
     CDS             2426..3238
                     /locus_tag="ACHK3A_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFO8742893.1"
                     /translation="MRKIIKYIVPVCLIFSVTGFVHAEIAGKGSVSDKRIQTALYSPD
                     NVFRIYAMKDRTTMIQLEQGETINTDAGAMNLGKPGTEGAPEWLLGANKAGSLIMIKP
                     SRYAEDPRTNLIVSTNRRTYLFELHLTQSPALATYLFRFNYPKPPEVGKNPFVGRNIN
                     ENPCDGIINKKYQKRGDMIISPYQVWDNGTFTCFRFPTNAPRPVIYEVLPDGTETMVN
                     ARPVNDILVVHGVSREFRIRLNRLVLAVRSKVENTGWYNYKGTTTGEVREVK"
     gene            3238..>3540
                     /locus_tag="ACHK3A_004954"
     CDS             3238..>3540
                     /locus_tag="ACHK3A_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HFO8742894.1"
                     /translation="MTENVVTPPENETKKETSAADENNPQGQGVGAFSLKPAGRKKPV
                     NWKIVGVIIGGIVIVLLFTIIFAAIVINNRADPEPEIDETGIKAEVVTRSIGVIDDP"
BASE COUNT         1131 a          497 c          793 g         1119 t
ORIGIN      
        1 ttttgaaatt gtgcttttta atgttgcttt tgtctggatg taatgataaa gcaaaagact
       61 ttataggagg gtggactcag gaatcaaatg aagaatatcc agtatatata accataaact
      121 atgatagcgg aatttatcat gtggatgtta aaaaactaga tacttatctg gtagaaaaga
      181 aaaaagctga tgcatttgat gatttcatgt gggggaaaaa aagtaagaat gaatctgaaa
      241 atttaacatt tgatgattgt tataaagtgt tcaattttga agctaaagca ataagtagtt
      301 cggtattaga aggtaagggt atatctgtaa ggatggatgg tgatagatta atttttgatg
      361 gaaaaagatt tgtaaggaaa aaatcataat gtgacatagc taaaggattt ttatatgcag
      421 gtaaatgtcg cggaaacgct gtttagaaca attgataccg cgacacaatc tcagatggca
      481 aatatgaata ctgtcatgtc tgttatgggg attgtgatag gcggtatatg gatgacatat
      541 atattaatga agtcattata ttggtatttt gagggcttaa caacagtaat acaggacgca
      601 ttttttacaa tatttaaagc atttgcaatc atgatggcat cattgactat atcatggtat
      661 acatcaacag ttattcctcc agttttagaa ttccctttat ggttggctaa tacaattgca
      721 ggagttaaca atgattcgtc aaatttagtt gatatggtaa taaattcata tatatcagga
      781 ctggaaaatc tggttgataa aatgaaattt ggattttctg tatcatcaat aaaaagtatg
      841 gttactgtcg gtgttgctgt tgttttatat ttactaagtg gattgccttt tttaggggtg
      901 gctatagcaa cattgctgac gcttaaagtc gcaacaaatc taattcttat cattggtcca
      961 atgtttattg catgtttagc ttttcctaaa gtagctcatt ggttttgggg atgggtggga
     1021 gttttaggag gatttgttct tacacaggcc ctttttgcta ttactcttgg aatggctatt
     1081 tcatttatta atagcaatat gattaataat ggggacttta atatatcaat ggttgatatt
     1141 atatgtatgt ctttatattt tagtgcattt actttactgg ctacagaaat tccaaattat
     1201 gctgcatcta ttatgggagg cgctccatct ggtggggtag ggagtatgtc caggctttta
     1261 ggaaaagcaa ctggttttgg tgctgcaagt cacatgagta aggcattagg aagtaaatta
     1321 gttgatcgtt ttcatggtag agggaaaggt cgtatttcct agtaattttt ctatctctgc
     1381 ttataatcca ttttctcgtc ttctttacaa aaacttcatt tataaaaagg cttatcatga
     1441 aaaatatatt cgctatatgt gcggcgcttt gtgttgtgct gggatgcata tcatgtagtt
     1501 caccgccaga gttgtcccaa ccatctggtg aatggattga tgtaaatcca gagtctgtgc
     1561 tgttaccggg agaaaaaaat gttatttaaa agtaataaga taaaaaagga aactgaacag
     1621 gaacgtctct tgcattatgg tgacggcaaa ttatcaggta agagtgatga aattgggagg
     1681 gaaatacggg cttacacaat ggctgcccgt tggtttgaaa aaagggtggc tgaagattac
     1741 agaaagaaag ctagaaatag tcgtattttg gctggattgt ttggtgttat agctattgtt
     1801 gctgtaattg cagttgcaaa tttagctcca cttaaaacag tagagccttt tgttatccga
     1861 gtagatcgca atagtgggta tatggatgtt gttcgtcccg gatggagtaa ggaagataca
     1921 aaagatattt ctgatgataa gcattttata tcattatata ttttatctag agaaacgtat
     1981 aactgggcta gtcaaaaatc taattttgca atagtaaggc agttgtctta ttccgatgtg
     2041 ttttctgaat atcgtaattt tcagttgtca ggtaaagggt acgtctcaac attaaatcag
     2101 tctcgtcaga tttctgttga tattgattca atagttcctt tacctgtcag tactgataaa
     2161 aaattaggtg agagggatga catcaaaact tatcaggttc gtttcagaca atcactgctt
     2221 gatgcagaag ggcggcctgt tccagagaca ggacagatac tgaagttgga tgaaaacggg
     2281 aaaccaatag ttaagcccaa agtggttttc tggacagcaa taatatcttt tgattattta
     2341 aatacggctg atacggaagg tgaagcctgg ttaaatccta agggctttgg tgtgcttgca
     2401 tatacaaaaa cagaggagat caggaatgag gaaaataatt aaatatatag ttcctgtatg
     2461 tcttatattt tcagttactg gttttgttca tgcggaaata gccggaaaag gttcagtcag
     2521 cgacaaaagg atccagacgg cattgtatag tcctgataac gttttcagaa tttacgccat
     2581 gaaggacaga acaacaatga tacaactgga acaaggggag acaataaata ctgatgcggg
     2641 tgcaatgaat ctggggaaac caggaacaga gggggcacct gaatggttac tcggtgctaa
     2701 taaggccgga agtttaataa tgataaaacc aagtcgatat gcggaggatc ccagaacaaa
     2761 cttaattgta agtacgaatc gccggacata tttatttgaa ttacatctga cacaatcacc
     2821 ggctttagca acatatctgt tcaggttcaa ttatccaaaa ccgccagaag ttggtaaaaa
     2881 tccatttgtt gggaggaata taaacgaaaa cccgtgtgat ggtattatta ataaaaaata
     2941 ccaaaaacgt ggtgatatga ttatttcgcc ttatcaggta tgggataatg gtacatttac
     3001 ctgttttcgt tttccgacaa atgcgccacg tccagttatt tatgaagttc tacctgatgg
     3061 tacagaaact atggttaatg cgcgtcctgt aaacgatatt ttggttgtgc atggtgttag
     3121 tcgtgagttc agaattcgtc ttaacagact ggttcttgct gtacgttcta aagtagaaaa
     3181 tacaggatgg tacaattata agggaacaac tacaggtgaa gtcagggagg tcaaataatg
     3241 actgaaaatg tagtgacacc accagagaat gaaacgaaaa aggaaactag cgctgctgat
     3301 gaaaataatc ctcaaggtca aggtgtaggc gcattttctt taaaaccagc tggtcgtaag
     3361 aagcctgtta actggaaaat agttggtgta attattggcg gtattgttat tgttcttttg
     3421 tttacaatta tatttgccgc aatagttatt aataatcgtg cagatccaga accagaaata
     3481 gacgaaacag ggattaaagc ggaggtcgta actcgctcta taggggtaat tgatgatcca
//
LOCUS       DAZGHF010000257         3526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGHF010000257 DAZGHF010000000
VERSION     DAZGHF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.259"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1599
                     /locus_tag="ACHK3A_004955"
     CDS             <1..1599
                     /locus_tag="ACHK3A_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFO8742895.1"
                     /translation="PESLCDVFTWHKLCHQARNKLSLSPESPAGWFESGCCEDLLNAL
                     NKNKLRDYDALIIDEAQALHSSWLETLSCWFENKRIIAFCDETQTFSFEKGTNYADLQ
                     RIINPQFSFNLSVVLRCPRAVTDYLIAVSPASYQITSPRNNEPDTLKELVVTDPLSTL
                     LNEILQLKESSVLPDEITVLVPTDLVKNSLQNDMRTLGVNIETVSRFRGMESSVVITL
                     YSESMEDAQLFCAYSRATTAFIAIYDSEKLAWREKNKFLHKLVNRKEVQRAINEAKHN
                     SSSSVLMAGFFREGELKLSTVKLAWSKHFSSWLVEFDERNSPEEMWIDYLYSYLPYPI
                     IYWFRDSCRAVNYIKNKQFELNELGSGIHLVIKNCSICKKMVPFYSDDNCLLCNKKNE
                     ELSPTRDEINKIREFDVTISQDNLNSVTQRLLPTPLAAVRARRVAFENPYRNKVLSES
                     LPAGNLLYRSALAFIQALIALVPSKKILDLDKITDALRRRYVHLEQLEYAELRNALAC
                     ALSTCYNRKYLIKVAKGKYLPVDD"
     gene            1681..2028
                     /locus_tag="ACHK3A_004956"
     CDS             1681..2028
                     /locus_tag="ACHK3A_004956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8742896.1"
                     /translation="MPGQPIPSELKEKGLIWLLPPYCWSHRQIARKLKISASVVSKWR
                     YELVEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLFVEQVK
                     EWRAISIQAHEKN"
     gene            2201..2365
                     /locus_tag="ACHK3A_004957"
     CDS             2201..2365
                     /locus_tag="ACHK3A_004957"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8742897.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(2369..2836)
                     /locus_tag="ACHK3A_004958"
                     /pseudo
     CDS             complement(2369..2836)
                     /locus_tag="ACHK3A_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2900..3184
                     /locus_tag="ACHK3A_004959"
     CDS             2900..3184
                     /locus_tag="ACHK3A_004959"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8742898.1"
                     /translation="MEQKTLSAEPRRSFSNNFKLQMVKLALQPRVSVARIARKHDIND
                     NLLFKWLRLWLIKGRISRRLPVRNSSGIGVELLPVEMTLDERPYPAFYAR"
     gene            complement(3401..>3526)
                     /locus_tag="ACHK3A_004960"
     CDS             complement(3401..>3526)
                     /locus_tag="ACHK3A_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO8742899.1"
                     /translation="LLITFLPVFLLPVLGNIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT         1064 a          715 c          767 g          980 t
ORIGIN      
        1 ccggagtcat tatgcgacgt ttttacctgg cacaaacttt gtcatcaggc tcgaaacaaa
       61 ttatccctgt cccccgagtc tccggcaggc tggtttgaat caggatgctg cgaagatttg
      121 cttaatgcat taaataaaaa taaactaaga gattatgatg ctttaatcat tgatgaagca
      181 caggcgttac attcttcgtg gctggagact ttatcttgtt ggttcgaaaa taaaaggatt
      241 atcgcatttt gtgatgaaac tcaaacattt tcctttgaaa aaggaacaaa ttacgctgat
      301 cttcagcgaa taataaatcc ccaattttct tttaacttat ctgttgtgtt aagatgtcct
      361 agagccgtta ctgattattt aatagctgtc agccctgcct cataccaaat tacttcccct
      421 aggaataacg aaccggatac gctaaaagaa ttagttgtta cagatccact ctccacattg
      481 cttaatgaaa ttttacaatt gaaagaaagt tctgtactgc ctgatgagat tactgtcctt
      541 gttcctacgg atttagtgaa aaattccctc cagaatgata tgagaacttt gggggtgaat
      601 attgaaacag tatctcgttt tcgaggtatg gaatcatctg tcgtgattac attgtattcc
      661 gaaagcatgg aggatgcaca gttattttgt gcttattcaa gagctacaac tgcttttatt
      721 gcaatttatg attcggagaa attagcatgg agagaaaaaa ataaattttt gcataaactg
      781 gttaacagaa aggaggttca gagagcaatt aatgaagcaa agcataattc ttcctccagt
      841 gttcttatgg ccggtttttt ccgcgaagga gagttaaaac ttagtactgt aaaattagca
      901 tggtcgaagc acttttcttc gtggcttgtt gaatttgatg aaagaaattc tccagaagaa
      961 atgtggatag attatttgta ttcctatttg ccttacccga ttatatattg gttcagagat
     1021 tcatgtagag ctgttaatta cataaaaaat aaacaatttg aattaaatga attaggctca
     1081 gggatacatc tggtaataaa aaattgtagc atatgtaaaa agatggtacc tttttattct
     1141 gatgataatt gtttgctttg taataagaag aatgaagagt tgtcaccaac cagagatgaa
     1201 attaataaaa ttcgcgagtt tgatgtaaca atatcccagg acaatcttaa ttcagttact
     1261 caacgattgt tacccacacc attagctgcg gtaagagccc gaagagttgc atttgaaaat
     1321 ccatatagaa ataaagtact aagtgaatca ttacctgctg gaaaccttct ttacagaagt
     1381 gcattagctt ttatacaggc cttgattgca ttagtcccat caaagaaaat actggacttg
     1441 gataagataa cagatgctct gcgtaggcgg tatgttcatc ttgagcaatt agaatatgcc
     1501 gaattaagaa atgctttagc ttgtgcattg tcaacttgtt ataatagaaa atatctcatt
     1561 aaagttgcaa aaggaaaata tttacctgtt gacgactagg tgcgtattac cgtgaaggag
     1621 atcggtgagt aaccccctgt gtcagcatag ttgtcgcctc aattttttaa ggagataaca
     1681 atgccaggac aaccaatacc aagtgagctc aaagagaaag gactgatatg gcttttacca
     1741 ccatactgct ggtcacatcg tcagatagcc cgtaagctaa agatcagtgc ctcagttgta
     1801 tcaaaatgga gatatgagtt agtggagcaa gggcttttac ctgaaaatga aaagattacc
     1861 tccgttgatg ttgaagactg gacaccggag cgtcgattct cagttgttct tgagacggct
     1921 acgctgtcag aaatggagct tgctgattat taccgacgca tgggattgtt tgttgaacag
     1981 gttaaagaat ggcgggctat ctcaattcag gcgcatgaaa aaaattgacg gaaaatattc
     2041 gaacagacag ggcattgcgt gacgcaaaaa ataagttacg agaactgtag aaagagctta
     2101 agcgtaaaga taaagctctg gcggaagctg cggccctgct tatattgagg gaaaagttca
     2161 atgccctctg ggacaacagc gaggaagact gacccctctc ctggagcggc gtaaaattat
     2221 tgcccttgtc cgcgaggccg tctccaacgg cgcccggaaa agtcaggctt gtaggtgtat
     2281 cggtatttca attcggacac ttcagcgctg gactgatggc aacgttatta ccacggatgt
     2341 aagcgtcaac ggagcaccgt attgacgctt atttattggt gagtactacg ttccatggca
     2401 ggagttcatc aactcggttg gaaggccatt ccggcagtac gctcaggata aggcgcagat
     2461 acgcttccgg atcgataccg ttcagacggc aggtgccgat cagcccgtac aacagtgctc
     2521 cacgctcgcc gccgtgatcg ctgccaaaga acataaagtt tttctttccg agacagactg
     2581 cacgaagcgc tctttccgca gcattattat ccgcctccgc cagaccgtca tcactgtaat
     2641 aacagagggc atcccactga ttcagtacat agctgaacgc ttcgcccagt ctggattttt
     2701 tcgacagcgt accattcttc tccaccatcc attcatgcag cgacgtcagt aacactttgc
     2761 ttcgctgctg cctgacggaa agacgctctg actccggtaa tccccgtatt tcgtaagcgt
     2821 aaaccgaccg ccgtatgtag ccattaagcc agtattggta acgtaggtgc ccaccttttc
     2881 aaactagtgg acgcctgtta tggagcaaaa aacattatcc gcagaacccc gcagatcatt
     2941 ttcaaataat tttaaacttc aaatggttaa actggcttta caaccaaggg tttctgttgc
     3001 ccgtattgcc cggaagcatg atatcaatga taatcttctg ttcaaatggc tcaggctctg
     3061 gctgatcaaa gggcgtatat cacggcgtct tccggtaagg aactcttctg gcattggcgt
     3121 tgaattatta cctgtggaga tgacgctgga tgagcggcct taccccgctt tttacgcgag
     3181 gtgattatga agatcaatga ctagccgtcg aaccgtgtgc atgaactgct gcactgaaac
     3241 ttctcagctg taaaataatc cttacgctac gtacttcccg gagcgcttac gattaatcgg
     3301 atgcagtcct gactggaaga aagaaaaatg ggaaaaaata agaggaatta atatcctcca
     3361 cgttcctgaa atcctggagg atattaatgt catcaaaata ttagcgaacg ataatcccta
     3421 acaaaatacc cagtgcgcca aagtcagcat cactgaaggt ggtgttagca aaaccaatgt
     3481 tacccagcac aggcaataag aatactggca ggaaggtaat aagcaa
//
LOCUS       DAZGHF010000258         3487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGHF010000258 DAZGHF010000000
VERSION     DAZGHF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.260"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            193..711
                     /locus_tag="ACHK3A_004961"
     CDS             193..711
                     /locus_tag="ACHK3A_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO8742900.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            921..3029
                     /locus_tag="ACHK3A_004962"
     CDS             921..3029
                     /locus_tag="ACHK3A_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO8742901.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQVIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLMVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            3096..>3487
                     /locus_tag="ACHK3A_004963"
     CDS             3096..>3487
                     /locus_tag="ACHK3A_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO8742902.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGIFGPGWKAPSDI
                     RLQIRDDALVLNDNGGRSIHFEPLLPGEA"
BASE COUNT          862 a          895 c         1005 g          725 t
ORIGIN      
        1 aacttattgc tgaataagag atttaatgat tttaaaaata gtttttacga caaccgtaaa
       61 ttctagctcg gtttgtatgt aaaaaatact gaaaaatgat gcggttccgt gaagagagct
      121 gcatctgcct gtctgggatt aacggcaggt cattgtaagt agtaattaac cacgttaaca
      181 aggagtttac aaatgccaac accgtgttat atctccatca ctggtcagac ccagggaaat
      241 attactgcag gtgcatttac cgctgattct gtcggcaaca tctatgtgca ggggcacgaa
      301 gacgagatgc tggtgcagga attcctgcat aacgttactg tcccgaccga tcctcagtcc
      361 ggccagcctg cgggtcagcg tgctcacaag ccgttcattt ttaccgtggc gctgaacaaa
      421 gctgttccgc tgatgtacaa cgcactggcc tccggcgaaa tgctgccaac cactgaactt
      481 cactggtggc gtacctctgt ggaaggcaaa caggagcatt atttcaccac ccgcctgacc
      541 gattcgacga ttgtcgatat gaaactgcat atgccgcatt gccaggatcc agcgaaacgc
      601 gagttcaccc agttgctcga agtttcactg gcctaccgca aaatagagtg ggaacacgtc
      661 aagtctggca cctccggtgc cgacgactgg cgcgcaccgc tggaagcata agttaagcca
      721 acagcatccg gctatcaggc cggatgctgt ttttgtatta cgccgtaaaa agaaaggttg
      781 tcagtaaggg attacgttca cgggagatgg cggatgccgg aaacttcggc ggtagcgtgc
      841 ctgaacgcaa gcgtaacact gtactgttac agccgcttta acagcgaact gtctgttttt
      901 ccttaaaccc ggaggttgct atgtcaaccg gattacgttt cacgctggaa gtggacggtc
      961 tgccaccgga cgcgtttgcg gtggtctcct ttcatctgaa ccagtcactc tcttcgctct
     1021 tttccctcga tctctctctg gtcagccagc agtttctctc ccttgaattt gcgcaggtgc
     1081 tggacaaaat ggcctacctg acggtatggc agggcgatga cgtacagcgc cgggtgaaag
     1141 gtgtggtgac ctggtttgaa ctgggggaga acgacaaaaa ccagatgctg tacagcatga
     1201 aggtgtgccc accgctgtgg cgcacagggc tgcgccagaa cttccgtatc ttccagaatg
     1261 aggacatcga aagcatactc gcgacgatcc tgaaagaaaa cggggtgacc gagtggagtc
     1321 cgctgttcag cgagccgcat ccttcccgtg agttttgtgt ccagtacggc gaaaccgatt
     1381 acgatttcct gtgccggatg gcggcggagg aaggcatctt cttttatgag gagcacgcgc
     1441 aaaaaagtac cgaccagagc ctggtcctgt gcgacaccgt gcgttatctg ccggagtcct
     1501 ttgagatccc ctggaatccg aacacccgta ccgaggtgag caccctatgc atcagccagt
     1561 tccgctacag cgcacaaatc cgcccatctt ccgtggtgac caaagactac acctttaaac
     1621 gacccggctg ggcagggcgt tttgatcagg aaggccagca ccaggattac cagcgcacac
     1681 agtatgaagt gtatgactac cccggacgtt tcaagggtgc ccacgggcag aactttgccc
     1741 gctggcagat ggatggctgg cgaaacaacg cagaagtggc gcgcggaaca agccgttcgc
     1801 cggagatatg gccgggacgg cgaattgcgc tgacggggca tccgcaggcg aacctgaacc
     1861 gggaatggca ggtggtggca agtgaactgc acggcgagca gccgcaggcg gtgccgggac
     1921 gcaggggttc aggtaccacg ctgaataacc actttgcggt aataccggca gacagaacat
     1981 ggcggccaca gccgttgctg aaaccgctgg tggacggccc gcagagcgcc gtcgtgacgg
     2041 gaccggcagg agaagaaatc ttctgtgatg aacatggccg cgtgcgggtg aaatttaact
     2101 gggaccgcta taacccgtca aaccaggaca gttcatgctg gatccgtgtg gcacaggcgt
     2161 gggcaggcac cggctttggc aacctggcga taccgcgtgt gggtcaggaa gtcattgtgg
     2221 acttcctcaa cggcgatccg gaccagccga ttattatggg gcgtacctac caccaggaaa
     2281 accgcacccc cggcagcctg ccgggaacaa agacgcagat gaccattcgt tcgaaaacct
     2341 ataagggcag cgggtttaat gaactgaagt ttgacgatgc gacagggaaa gaacaggtct
     2401 acatccacgc gcaaaagaac atgaacaccg aggtgctgaa taaccggacc acggatgtga
     2461 taaacaacca tgctgagacc atcggcaaca atcaggtgat tgcggttacc aacaatcaga
     2521 tacagacggt gggcgttaac cagatagaga cggtgggcag taaccagatc attaaagtgg
     2581 gctctgttca ggtggaaacg attggacttg ttcgtgcgct gaccgtgggc gtggcgtatc
     2641 agacgacggt aggtggcatt atgaacacct cggtggccct gatgcagtcc tcgcagatcg
     2701 gtttgcataa atcgttgatg gtcgggctgg gttatgacgt caaagtcgga aataacgtta
     2761 ccttcaccgt tggtaaaacg aaaaaggatg ataccggaca gaccgcgatt tactccgccg
     2821 gtgagcatct ggaactctgc tgtggtaagg caaggctggt gctgactaag gacggacaaa
     2881 tttttctcaa cggcacaaaa attcatttgc agggtaagga gcaggttaat ggtgactcac
     2941 tgttgattaa ctggaactgt gcagcctcga aatccccacc gaagacccct gatgaaaagc
     3001 aggatacgcc ggatatgaga gagtactgat cccgtacctg ttcggtattc agtaacaaac
     3061 aagcattaaa aataaacaga agaaggaatt gaatcatgag cggaaaacca gcggcgcgtc
     3121 agggagatat gactcagtat ggcggtccca ttgtccaggg ttcggcaggt gtaagaattg
     3181 gcgcgcccac cggcgtggcg tgctcggtgt gtccgggcgg gatgacctcg ggcaacccgg
     3241 taaatccgct gctgggggcg aaggtgctgc ccggcgagac ggaccttgcg ctgcccggcc
     3301 cgctgccgtt cattctctcc cgcacctaca gcagctaccg gacgaagacg cctgcgccgg
     3361 tggggatttt tggccccggc tggaaagcgc cttctgatat ccgcttacag atacgcgatg
     3421 atgcactggt actcaacgac aacggcggac ggagcattca ctttgagccg ctgctgccgg
     3481 gtgaggc
//
LOCUS       DAZGHF010000259         3476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGHF010000259 DAZGHF010000000
VERSION     DAZGHF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.261"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1548
                     /gene="tssF"
                     /locus_tag="ACHK3A_004964"
     CDS             <1..1548
                     /gene="tssF"
                     /locus_tag="ACHK3A_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO8742903.1"
                     /translation="LVSLLWPHYMRTIPSLAIVEFSPDWRSLRQAEILAEGFSVLSRP
                     VGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRFECPEKVDWSKAGIDKV
                     AIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDNDCLWKKA
                     DTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAASTWFEIDIVLSEAWSSCLPFET
                     ENFRLHCAPVINLFTLEADPLLLNPLDNEYLLRPLRLQDGHTEIYSVDNIHGSVKNGR
                     HPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLVLGGRSFELEQLSDKPE
                     SLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVLNLTAPTMPLYPPANDRFHWRV
                     MSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRRRLEAIVAVKHTLIRRFEKGFMLR
                     GVDIEVTLNMDNFAGEGDVNLFGEMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKEN
                     HSRYVPG"
     gene            1512..2600
                     /gene="tssG"
                     /locus_tag="ACHK3A_004965"
     CDS             1512..2600
                     /gene="tssG"
                     /locus_tag="ACHK3A_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO8742904.1"
                     /translation="MERESQPVRTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIGNRSGLGAGNRIRLSQRAPLGKTTGEACSRLLMTLETADPNEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPPAEDGHYRFE"
     gene            2636..3160
                     /gene="tssJ"
                     /locus_tag="ACHK3A_004966"
     CDS             2636..3160
                     /gene="tssJ"
                     /locus_tag="ACHK3A_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO8742905.1"
                     /translation="MVTVIRWWVPMFIACGLLAGCGLTQTVTDGTISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDVAGYQMLLRNADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
     gene            3165..>3476
                     /locus_tag="ACHK3A_004967"
     CDS             3165..>3476
                     /locus_tag="ACHK3A_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO8742906.1"
                     /translation="MDNRSAFFDGQTRYFLEINGSAVKPDVLRFRGREALSEPFRWDI
                     GFTSRNANIPPEEVLMKYASLRMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLAL
                     LE"
BASE COUNT          725 a          978 c         1003 g          770 t
ORIGIN      
        1 ctggtgagtc tgctgtggcc gcactatatg cggactatcc cgtcgctggc catcgttgag
       61 ttctcgcctg actggcgtag tctgcgtcag gccgaaatac tggctgaagg cttctccgtt
      121 ttgtcccgtc ctgtcgggcc gcataaaacc gcctgtcagt accggacgac ccgcgatgtg
      181 ttgctacaac cgctgcacct tgcagatgcc cgtctccata ctgaatccga tggccgctct
      241 gccatccgcc tgcgcttcga atgccctgaa aaagtggact ggagtaaagc cgggatcgac
      301 aaagtcgcca tttttcttaa tgcagaggcg ccggtaagcg ctgctctgca tctggcaatg
      361 acccggcgtg tacatgccat gtatgcgcgt catgccggga catataccgg acgtcatcag
      421 tttgacggct ggtgcagacc catgggcttt gacgacaatg actgcctgtg gaagaaagcc
      481 gatacagcct tcagtggtta ccagttgctg ctggagtatt tcagcttccg tccgaagttt
      541 atgtttgttg aactgcgcgg cctcgacacc atcgggctca ctgcggccag tacctggttt
      601 gaaatcgaca ttgtgctcag tgaagcctgg tcatcctgtt tgccttttga gacagaaaac
      661 ttccgcctgc attgtgcgcc ggttatcaat ctctttaccc tggaagccga cccgctgctc
      721 cttaacccgc tggataacga gtacctgctc aggccgctgc gtcttcagga tggtcacacc
      781 gaaatttaca gcgtcgataa cattcacggc tcggtgaaga atggcagaca cccttatgtc
      841 ccgttcacca gcttccggca ccggggcggt atgatgcgcc acgatgctcc tgaacgttat
      901 taccacaccc gcgtgaaacg gggaccctcc ggcctgtatg acacatggtt agttctcggg
      961 ggacgttcgt tcgaactgga gcagttgtct gataaaccgg agtccctctc gatgcgaatc
     1021 accgcgacca acgggcagct tccgcgcagg gcgctggaaa gtacgctcct ggacagggtg
     1081 gtcaaaaccg gcaaagtccc cgtcagagtc ctgaacctga ccgcaccgac gatgccactg
     1141 tatcccccgg caaatgaccg gtttcactgg cgggtgatga gtcacctcgg ttcaaacttt
     1201 ctcagcatga tggacaatcc ggaggtgctg cgggggacgc tggcgctcta tgactggacc
     1261 gacgatgaaa tgaaccgccg ccggcttgaa gccatcgttg ccgtgaagca caccctgatc
     1321 cgccgttttg aaaagggctt tatgctccgt ggcgtggaca ttgaggtcac gctgaatatg
     1381 gataattttg ccggcgaggg cgatgtgaat ctcttcgggg agatgctgca ccgtttcttt
     1441 gcgctctatg ctgatgttca ccttttcaac cagctcacac tggtactgca acccacaggg
     1501 aaacgactga gatggaaaga gaatcacagc cggtacgtac cgggctgacg caggccctgg
     1561 cccttaacaa cgatatctgg cgggccagtt tttatcgttt ctgccagctg ctggagcagg
     1621 aaaatccgga cgggccaaaa ctgggcacga ccagccatcc gggcgatgat ccggtgcgat
     1681 tcagaccctg gcccgggatg ggattcccgg tcagcaccct gaaagcggtg gagatcgacg
     1741 aagaccgtcc gacattaccc ccaacaatcc gcacgacatt tctcggaatg tatggggtgg
     1801 actcacctct gccgacgtcc tggctggatg atattacgca gcgccgtgaa ggacatgagg
     1861 cactcacctc atttctggat attttcagcc atcgcatcac cacgcagtac taccggatct
     1921 ggcgaaaata tgcgtacccg gccacttttg aggagggggg gcgcgatgcc acatcgcagt
     1981 gtctgctggg gctggtcggg ctcggtatac ccggcaccgc tgaacaggtg gcaacgccgg
     2041 tttcccgttt cctggcctta ctgggtgcca tgcgattacc cacccgcaat gctgaaggca
     2101 tccgggctct ggtgagtctg ctggcaccgg atacccgcgc acttatcacc gaacccgatc
     2161 cggttaaggt ccatatcggt aaccgcagtg gactgggtgc cggaaaccgc atccgtcttt
     2221 cgcagcgggc gccgctgggt aaaacgacag gggaagcatg cagccggctc ctgatgacac
     2281 tggaaacagc agatccgaac gaagcggaag gctggttgcc cggtggtgtc ctccataccg
     2341 atttgctggt cctgctgcgt gtttatctcg gctaccgcag tgacgtccgg ctgcgactca
     2401 ctgtccccgt gcggttgttg cctgagcccc gcctgggcaa aggcaggcct gttcagctgg
     2461 gccgtaccgg tttgctgggg ctgaaagcgg gcaagctcag caatggccgg gagagtctga
     2521 cggtcagtct gggctgctac gaagggttga aatgtgctgc gctaccgcct gctgaagacg
     2581 gacattatcg cttcgaataa tcacctgttc tctctctcaa taaaggagcg cctgaatggt
     2641 aacagtaatc cgctggtggg tgccgatgtt tattgcctgt ggcctgctcg caggctgcgg
     2701 tctgacgcag accgtcacgg acggcacgat cagcatcacg aaatccatct tctacaaaaa
     2761 aatcaaaacg ctgcatctgg attttacccc acgcacggcc attaacactg acggggcaca
     2821 gacgccactg gcaaccatgg tgcgggttta ccagctcagg gatcgtaaag ccgttgatgt
     2881 cgccggttac cagatgctgc tgcgtaatgc cgatacggta cttaaggacg acgtgctggc
     2941 ctcaaaagag ctgctggtga tgcccggcgg cagcgtgacg ctcaatatgc ccatggatga
     3001 agatgcgcag tttgtggctg tggtgggatt gtttaaccgg ccggatcaga aggacaatcg
     3061 ctggcgcctg gtactgaccc gtgatgacct caatccggat aagccccgca ccattgaact
     3121 gggtgatggc tggctcagcc ttgtgccggt aaaggagtga cgtgatggat aaccggtcag
     3181 cgttttttga cgggcagacc cgttattttc tggaaattaa tggcagtgca gtaaagcctg
     3241 atgttttacg ttttcgtggc agggaagcac tgagtgagcc gttccgatgg gacatcgggt
     3301 tcacctcccg gaatgcgaac attcctccgg aagaggtgct gatgaaatat gcgtcgttgc
     3361 ggatgcgtaa cggcaaaaat gtccatggca ttgtgacccg tctggaatgg ctgtccacat
     3421 cgaaagacca gtcccactac cggcttacac tcagctcccg tctggcactg ctggag
//
LOCUS       DAZGHF010000260         3474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGHF010000260 DAZGHF010000000
VERSION     DAZGHF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.262"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1548
                     /gene="tssF"
                     /locus_tag="ACHK3A_004968"
     CDS             <1..1548
                     /gene="tssF"
                     /locus_tag="ACHK3A_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO8742907.1"
                     /translation="LVSLLWPHYMRTIPSLAIVEFSPDWRSLRQAEILAEGFSVLSRP
                     VGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRFECPEKVDWSKAGIDKV
                     AIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDNDCLWKKA
                     DTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAGSTWFEIDIVLSEAWSSGLPFET
                     ENFRLHCAPVINLFTLEADPLILNPLDNEYLLRPLRLQDGHTEIYSVDNIHGSVKNGR
                     HPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLVLGGRSFELEQLSDKPE
                     SLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVMNLTAPTMPLYPPANDRFHWRV
                     MSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRRRLEAIVAVKHTLIRRFEKGFMLR
                     GVDIEVTLNMDNFAGEGDVNLFGEMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKEN
                     HNLHVPG"
     gene            1512..2600
                     /gene="tssG"
                     /locus_tag="ACHK3A_004969"
     CDS             1512..2600
                     /gene="tssG"
                     /locus_tag="ACHK3A_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO8742908.1"
                     /translation="MERESQPARTGLTQTLALNNDIWRASFYRFCQLLELENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRVLITEPDPV
                     KVHIDNRSGLGAGKRIRLSQRAPLGKTTGEACSRLLMTLETADPDEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPSAEDGHYRFE"
     gene            2634..3158
                     /gene="tssJ"
                     /locus_tag="ACHK3A_004970"
     CDS             2634..3158
                     /gene="tssJ"
                     /locus_tag="ACHK3A_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO8742909.1"
                     /translation="MVTVIRWWAPMFIACGLLAGCGLTQTVTDGTISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDAADYQMLLRRADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLILTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
     gene            3163..>3474
                     /locus_tag="ACHK3A_004971"
     CDS             3163..>3474
                     /locus_tag="ACHK3A_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO8742910.1"
                     /translation="MDNRSAFFDGQPRYFLEINGSAVKPDVLRFRGREALSEPFRWDI
                     GFTSRNANIPPEEVLMKYASLRMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLAL
                     LE"
BASE COUNT          729 a          971 c         1001 g          773 t
ORIGIN      
        1 ctggtgagcc tgctgtggcc gcactatatg cggactattc cgtcgctggc catcgttgag
       61 ttctcgcctg actggcgtag tctgcgtcag gccgaaatac tggctgaagg cttctccgtt
      121 ttgtcccgtc ctgtcgggcc gcataaaacc gcctgtcagt accggacgac ccgcgatgtg
      181 ttgctacaac cgctgcacct tgcagatgcc cgtctccata ctgaatccga tggccgctct
      241 gctatccgcc tgcgcttcga atgccctgaa aaagtggact ggagtaaagc cgggatcgac
      301 aaagtcgcca tttttcttaa cgcagaggcg ccggtaagcg ctgctctgca tctggcaatg
      361 acccggcgtg tacatgccat gtatgcgcgt catgccggga catataccgg acgtcatcag
      421 tttgacggct ggtgcagacc catgggcttt gacgacaatg actgcctgtg gaagaaagcc
      481 gatactgcct tcagtggtta ccagttgctg ctggagtatt tcagcttccg tccgaagttt
      541 atgtttgttg aactgcgcgg cctcgacacc atcgggctca cggcgggcag tacctggttt
      601 gaaatcgaca ttgtgctcag tgaagcctgg tcatccggtt tgccttttga gacagaaaac
      661 ttccgcctgc actgtgcgcc ggttatcaat ctctttaccc tggaagccga cccgctgatc
      721 cttaacccgc tggataacga gtacctgctc aggccgctgc gtcttcagga tggtcacacc
      781 gaaatttaca gcgtcgataa cattcacggc tcggtgaaga atggcagaca cccttatgtc
      841 ccgttcacca gcttccggca tcggggcggt atgatgcgcc acgatgctcc tgaacgttat
      901 taccacaccc gcgtgaaacg gggcccctcc ggtctgtatg atacatggtt agttctcggg
      961 ggacgctcgt tcgaactgga gcagctgtct gataaaccgg agtccctctc gatgcgaatc
     1021 accgccacca acgggcagct tccgcgcagg gcgctggaaa gtacgctcct ggacagggtg
     1081 gtcaaaaccg gcaaagtccc cgtcagagtc atgaacctca ccgcaccgac gatgccactg
     1141 tatcctccgg caaatgaccg gtttcactgg cgggtgatga gtcacctcgg atcaaacttt
     1201 ctcagcatga tggacaatcc ggaggtgctg cgggggacgc tggcgctcta tgactggacc
     1261 gacgatgaaa tgaaccgccg ccggcttgaa gccatcgttg ccgtgaagca caccctgatc
     1321 cgccgttttg aaaagggctt tatgctccgt ggcgtggaca ttgaggtcac gctgaatatg
     1381 gataattttg ccggcgaggg cgatgtgaat ctcttcgggg agatgctgca ccgtttcttt
     1441 gcgctctacg ctgatgtcca ccttttcaac cagctcacac tggtactgca acccacaggg
     1501 aaacgactga gatggaaaga gaatcacaac ctgcacgtac cgggctgacg cagaccctgg
     1561 ctcttaacaa cgatatctgg cgggccagtt tttatcgttt ctgccagctg ctggagctgg
     1621 aaaatccgga cgggccaaaa ctgggcacga ccagccatcc gggtgatgat ccggtgcgat
     1681 tcagaccctg gcccgggatg ggattcccgg tcagcaccct gaaagcggtg gagatcgacg
     1741 aagaccgtcc gacattaccc ccaacaatcc gcacgacatt tctcggaatg tatggggtgg
     1801 actcacctct gccgacgtcc tggctggatg atattacgca gcgccgtgaa ggacatgagg
     1861 cgctcacctc atttctggat attttcagcc accgcatcac cacgcagtac taccggatct
     1921 ggcgaaaata tgcgtatccg gccacttttg aggagggggg acgcgatgcc acatcgcagt
     1981 gtctgctggg gctggtcgga ctcggtatac ccggcaccgc tgaacaggtg gcaacgccgg
     2041 tttcccgttt tctggcctta ctgggtgcca tgcgattacc cacccgcaat gcggaaggca
     2101 tccgggctct ggtgagtctg ctggcaccgg atacccgcgt acttatcacc gaacccgatc
     2161 cggttaaggt ccatatcgat aatcgcagtg ggctgggtgc cggaaaacgc attcgtcttt
     2221 cgcagcgggc gccgctgggt aaaacgacag gggaagcatg cagccggctc ctgatgacac
     2281 tggaaacagc agatccggac gaggcggaag gctggttgcc cggaggtgtt ctccataccg
     2341 atttgctggt cctgctgcgc gtttatctcg gctaccgcag tgacgtccgg ctgcgcctta
     2401 ctgtccccgt gcggttgttg cctgagcccc gcctgggcaa aggcaggcct gttcagctgg
     2461 gccgtaccgg tttactgggg ctgaaagcgg gcaaactcag caatggccgg gagagtctga
     2521 cggtcagtct gggttgctac gaagggctga aatgtgctgc gctaccgtct gctgaagacg
     2581 ggcattatcg cttcgaataa tcacctgttc tctctcaata aaggaacgcc tgaatggtaa
     2641 cagtaatccg ctggtgggcg ccgatgttta ttgcctgtgg cctgctcgca ggctgcggtc
     2701 tgacgcagac cgtcacggac ggtacgatca gcatcacgaa atccatcttc tacaaaaaaa
     2761 tcaaaacgct gcatctggat tttaccccgc gtacggccat taacactgat ggggcacaga
     2821 cgccactggc aaccatggtg cgggtttatc agctcaggga tcgtaaagcg gttgatgccg
     2881 ccgattacca gatgctgctg cgccgggccg atacggtact taaggacgac gtgctggcct
     2941 caaaagaact gctggtgatg cccggcggca gcgtgacgct caatatgccc atggatgaag
     3001 atgcgcagtt tgtggctgtg gtgggactgt ttaaccggcc ggatcagaag gacaatcgct
     3061 ggcgcctgat actgacccgt gatgacctca atccggataa gccccgcacc attgaactgg
     3121 gtgatggctg gctcagtctt gtgccggtaa aggagtgacg tgatggataa ccggtcagcg
     3181 ttttttgacg gacagccccg ttactttctg gaaattaatg gcagtgcagt aaagcctgat
     3241 gttttacgtt ttcgtggcag ggaagcactg agtgagccgt tccgatggga tatcgggttc
     3301 acctcccgga atgcgaacat tcctccggaa gaggtgctga tgaaatatgc gtcgttgcgg
     3361 atgcgtaacg gcaaaaatgt ccatggcatt gtgacccgtc tggaatggct gtccacatcg
     3421 aaagaccagt cccactaccg gcttacactc agctcccgtc tggcactgct ggag
//
LOCUS       DAZGHF010000261         3474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGHF010000261 DAZGHF010000000
VERSION     DAZGHF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.263"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..368)
                     /locus_tag="ACHK3A_004972"
     CDS             complement(<1..368)
                     /locus_tag="ACHK3A_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DotU/TssL family secretion system protein"
                     /protein_id="HFO8742911.1"
                     /translation="MNKPVISRAEQIFYPGWLMVSQLRCGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQESSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVA"
     gene            complement(365..1702)
                     /gene="tssK"
                     /locus_tag="ACHK3A_004973"
     CDS             complement(365..1702)
                     /gene="tssK"
                     /locus_tag="ACHK3A_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO8742912.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCKDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            complement(1718..3262)
                     /gene="tssC"
                     /locus_tag="ACHK3A_004974"
     CDS             complement(1718..3262)
                     /gene="tssC"
                     /locus_tag="ACHK3A_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO8742913.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDT
                     VPVRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDL
                     PIHLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPA
                     LYDTPDATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGL
                     VTEMTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQ
                     MPKAKA"
     gene            complement(3283..>3474)
                     /locus_tag="ACHK3A_004975"
     CDS             complement(3283..>3474)
                     /locus_tag="ACHK3A_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO8742914.1"
                     /translation="PEQVARQIPRLKAMLAMRSLLRDLKANLLDNATFRKELEKILRD
                     PELSQALRDELSALVPEQV"
BASE COUNT          856 a         1034 c          839 g          745 t
ORIGIN      
        1 gccacatccg gcgcaggtaa tttcagttgc tcacgaatac gctcccagag ctcttctccg
       61 gcattgagcg taccaaaaaa atgagcctgc agcgggtcct gctgccaggt gtgccagcca
      121 tcgtctgttt tttcacggtt cagcacactc tcgtccagca gggcgcagaa ggcatacaac
      181 atgatttcac tgctttcctg actgaattct gcttcagcca gctcttcacg cgcctgcgtc
      241 accagctgac aggcacggcg atacagggcc ttaccatcct caaccggttg tccgcagcgc
      301 agctggctga ccatcagcca gccgggataa aaaatctgtt cagcccggga gataacaggt
      361 ttattcatgt ccgcagtacc gcaaaaagtt ccagtttcac atcccccagc gatgccgggg
      421 tgtagaaagt acaacgcccc gcgtccagca tcgcccgggc tgcatccgta cttaaatcca
      481 gcgcgaaata ctggttttcc agccggagcg gaatggccgc cggtacatgc gtaaccggac
      541 ggataatgac cccgctcagc gccacattca ccacctcaga cacatcatcg gggcttccgg
      601 ctttgcatag ctgcggaaac ttcgtctgca gttcatgccc gggtatggaa gagcgcactg
      661 aaagccagaa atccgcccct tcgcgcaaac gcgcatcatg taatgccccc tcccacatca
      721 caccattttg tttcagttca atgaaaacca cacgggaagg caggcttgcc tccagcagct
      781 ggttcagcaa agaaagcaag ggtggaaaca cattttcagg tgtttcatga cggtatgccg
      841 gaaccgcatc cacattatgc tcaagcgaga atgtgagcag ggagcctgcc agccgggcaa
      901 gctccctgta caacagttcc gggtggcggt aaggcatgtc aagcaactct ttcagaacag
      961 gctccgcact gttcagggca ttcagcaacc agaacaacga cacgtccgcc actgcaaaat
     1021 ccgcaagacg ggcattgttc tcccgacgca tggacatcag tcgctggcga cgcgcctgca
     1081 ggtgatgaag caactccgca agctcagtca tcagcgaagg actggcagac agcgtcagca
     1141 gagggggaat aaaacgcggg tccttgcacc actgtccctg tgcatctcga acaagacggg
     1201 taacaggaca ggtcagccag gcagcatttt cctgatgagc catgcgcaga gtcaggttat
     1261 gccgcaatac cgccacttcg ctttgctcat gcccggccag ctcctgcaca ttcacccttt
     1321 ctgatttcca gcgacgcgga cgctcgctct cactgccatc atcaaggttg ccaccgttag
     1381 cattcagtaa cggtagcgcc agcacaatat ccaccaggga acgctcagaa accgtcgata
     1441 aatcacagac cggaggcaga ttatccgcac gttccgtatc aataagcgtt ccgtccggga
     1501 aacgaacaat cagacgggtg gcattcaacc gtgaaagcgg taacagagag tcatcaaact
     1561 ctgcagccac cacgccccag ggatgcgcca gccccattcg ggcaacgctg tctgccagat
     1621 gaacatccca ggcagcctgc tgctgaaact gctggggcat cagaaaagcc ccgtcttccc
     1681 acagtggacg ataaatcttc atttcccgac gcctgcctta cgcttttgcc ttcggcatct
     1741 gcgaaaccag tgacagactg acgtccatac cttccacctg gaaatgtggt acagcataga
     1801 gtttcacgcg gaaaaatccc gggttgtctt cgatgtcttc caccaccact tttccgtcac
     1861 gcagcggatg tgaggcctgc agttcatcac ccggatcggt catctccgtc accagacccc
     1921 ggatccagtt attcagttcc agttcaagca gacggcgatc tttggtggta ccgatattct
     1981 ccctctgaat gatcttcagg taatgcgcaa tgcgggacag caggaagata tagggaagac
     2041 gggcattaat acggctgttc gccgttgcat ccggcgtatc gtacagtgcc ggtttctggg
     2101 tggagttagc ggagaagaag caggcataat cccggttctt gtagtaagag agcggaataa
     2161 agcccagatt agcgaattca aactcccgag tttccggaat catcacctct gacggaattt
     2221 tgacctgatt accggttccc agatcataca gatggatcgg caaatcctgc accgcaccgc
     2281 cggcctgagg accgcggatc tgaacacacc agccgttggt gacaaaactg cgcaccatat
     2341 tggccgcaaa agcaaaagac gcatttgtcc agagatactt atgatgatcc ggacctttca
     2401 cctcttcaac atagttaaag ctgcggacag gtacggtatc aggaccatat ggcaggcgtc
     2461 ccagaacacg tggcatcacc agaccaatat agcgggcatc atccgtttca cggaaagcat
     2521 tccacttgat gtactcggca cgctcaaagt gattgccaat atccttaatg gcggccacct
     2581 cttccatcgt atccttccgg aagaaagccg ggccaactga accaataaat ggcatatgcg
     2641 cggacgcagc cacccgggaa atatttttca gcagcgccac atcctgcgca gacgcatcaa
     2701 actcccaggc agaaatcagg gctgcaatgg gttcaccacc cggcgtatcg tactccgcca
     2761 cataagtctg cagataaaga ccactctgaa taatttccgg cgcatcctca aaatcctgac
     2821 gcagatcgtc tttggacaga tccagcagtt caattttcac attacggcgg aagtccgttt
     2881 tatccaccag agacttcacg ccccgccaca gggactccac tttctgaaat tccggatgat
     2941 gaagcacggc atccagctga cggctgatct gataatcaag ctctgcaata tgatggtcaa
     3001 tcagcgcctt atcgagtttc tccaccggtg ctcccgcttt cgccaggcat tccataaaca
     3061 cctgcatccc tgcggtcaga cgttcatctg ctgatgcctc tgacatggcc tgcggatcct
     3121 gccagatatc cagcgcactc agggctgata ccggattcag attaattttt tcaaacaggg
     3181 aagcatatac gctgtcgccg gaatgcgttt cagaaaccgc atttcccgcc acattatttt
     3241 ctttctgtac agacatcagc atgctctctc ttaatccgtt aatcacacct gttccggcac
     3301 cagtgcactc agttcgtcac gtaaggcctg actcagttcg ggatcccgca gaattttttc
     3361 cagttcttta cggaaagtgg cgttatccag cagattcgcc ttcagatccc gcagaaggct
     3421 gcgcatggcc agcatggctt taagacgggg aatttgtcgg gcaacctgct ccgg
//
LOCUS       DAZGHF010000262         3445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGHF010000262 DAZGHF010000000
VERSION     DAZGHF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.264"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1102
                     /locus_tag="ACHK3A_004976"
     CDS             <1..1102
                     /locus_tag="ACHK3A_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFO8742915.1"
                     /translation="FKDGHTPEVRRRMVECVDRFYGEFKPWLKKTITKNWVGITDNNY
                     AKKRQEILDSTPEEIFSWYLSSAEKDFLAPDYSILIMGKRIFHNDNNRSVIKLTLPLS
                     LLKETDGKQRYEAWLMWLCDTFSVESGYAGLSFALPYARERMFPCEFALAQRFSGVMV
                     DSLGTLEGGGAVIGLKGACWYTILGTPWLEKLGGAERVKHRLAKTPEISLLPYNNGVV
                     LKAGELPPPLGEMKTEGLSPLLVKVNQVIKPVRFNESRSLHFYSEYEGLQFDKESTMK
                     WYRRFDEASALLDKEEMGKSCAPERIKCWTDEIAPHAGQWAAMVNGTTEYIQTREGQK
                     MPPFEDKYGKQHRACWKLLKRDDNGSVYTLPE"
     gene            1128..1507
                     /locus_tag="ACHK3A_004977"
                     /pseudo
     CDS             1128..1507
                     /locus_tag="ACHK3A_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
     gene            1504..2007
                     /locus_tag="ACHK3A_004978"
     CDS             1504..2007
                     /locus_tag="ACHK3A_004978"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO8742916.1"
                     /translation="MNSVSFSLAAQHLICSTKVTLFALVDGLQYERYFGESLSVQQPA
                     AVPLFDTWPDSRIAFAGPWVMEMNSIMDFRERLCELEAALPSVSWMISSSSLTELAAH
                     FRRNMNTELPDGRIALLRFHDPRVQKRLGEMLNDQQHRELTGLMQEWLTIVDGKAWSF
                     KQREFIC"
     gene            2001..2345
                     /locus_tag="ACHK3A_004979"
     CDS             2001..2345
                     /locus_tag="ACHK3A_004979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742917.1"
                     /translation="MLKLSEEQCVGMDKREDDDFVRDTALRLKKKFPGITESDDTFHV
                     RLRKALDYANTLPLKEKNVKRDFLFLEAFWPGFYLKPEVDKWLRTPNAYSTDQRVEDY
                     KHVMINRERREL"
     gene            2345..3109
                     /locus_tag="ACHK3A_004980"
     CDS             2345..3109
                     /locus_tag="ACHK3A_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tox-REase-5 domain-containing protein"
                     /protein_id="HFO8742918.1"
                     /translation="MVMVLTGAGMGSAATGGVGMAGASTGTAVFPGAGSRDGDWEEDN
                     ISYPGSGSGSRQGNGGFYYQGKQYDPILNEPMVVSAETHGDFWEDDMDHSETVNYEGI
                     DSKAEVQAKTDEKTCENCPPEGKVMPMIRRCSRWSMVTISYQTRICGTFYNPETQQIQ
                     EFKYCGVSFDGWKDKLCQFWEAKARYDQFFRADGSKKPWWTGNLSAINQASRHQAVAT
                     VNQPLKVVWIFMQPVSCEYFRKMFKDYKDIITRWQP"
     gene            3106..>3445
                     /locus_tag="ACHK3A_004981"
     CDS             3106..>3445
                     /locus_tag="ACHK3A_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742919.1"
                     /translation="MIELFNTSINIHSLKERDITIDDCLNELYRSTVVVKNLTQHDHI
                     WYLTGYSRKDASKHVVFSDNEPNQNTIDTFERHYKKNRPLINESIWNGQPDGLACSIS
                     HFMKFIDNPKK"
BASE COUNT          967 a          678 c          964 g          836 t
ORIGIN      
        1 ttttaaggac ggacatacgc cagaagtccg ccgcaggatg gtggagtgcg tggatcgctt
       61 ctatggggag tttaaaccct ggctgaaaaa aacgatcacc aaaaactggg ttggcatcac
      121 tgataataac tacgccaaaa agcggcagga gatcctcgat tcaacgcctg aagagatttt
      181 ttcctggtat ctgagtagcg cagagaagga ttttttagcc cccgattaca gtattttaat
      241 tatggggaaa agaatttttc ataatgacaa taatcgttca gtcattaagt taacactccc
      301 gttgtcgctg ctgaaagaaa ctgatggaaa gcagcgttat gaagcctggc tgatgtggct
      361 gtgtgacacc ttttcggtgg aaagtggtta tgcagggttg tcgtttgcac ttccttatgc
      421 ccgtgagcga atgttcccct gtgaattcgc cctggcgcag cgtttttccg gtgtgatggt
      481 ggattcactg ggaacgctgg aaggcggtgg ggcagtcatt ggtctgaaag gtgcctgctg
      541 gtacaccatt ctcggtacgc cgtggctgga aaaactgggg ggggcagaga gagtaaaaca
      601 tcgtttagct aaaacgcctg aaatcagcct gctaccctat aacaatggtg tcgtacttaa
      661 agccggtgaa ctccccccgc cactgggaga gatgaaaaca gaaggtttat cgccgctgct
      721 ggtgaaggtg aaccaggtca ttaagccagt gcggtttaat gaatcaagat ctcttcattt
      781 ctactctgaa tatgaaggac ttcaattcga caaagaatcc accatgaagt ggtacaggcg
      841 ctttgacgaa gccagcgccc tgctcgataa agaagaaatg gggaaatctt gcgcccccga
      901 acgcattaaa tgctggaccg atgaaatagc gccccatgcc ggacaatggg ccgcgatggt
      961 taatggcacc accgagtaca tccagacccg cgaagggcag aaaatgcccc cgtttgagga
     1021 taagtacggt aagcagcaca gggcttgctg gaaactactg aaacgagatg acaatggtag
     1081 cgtttataca ctacctgagt aaggtcgtgt gctgaatgac cgcgcaggcc ggagagaggc
     1141 tggggctttt tgcccggacc ggacaactga gcgtcaagtc cggggagggg ccggtagagg
     1201 ttcaggcgaa aaacggcagc atgcggttgt ttgctgagaa gaagctgaca ctgagctcag
     1261 cgtgtgatat ctcttttgcc gggaaaaaac gtatcacgct gattggcggg ggagttacct
     1321 gcgacttgaa gccgggaagg tcgagtatgg tacgacggca aagtatctgc gcaaagtgaa
     1381 gcggacaatg gcggcggggg ctgcatcaat accagtcagg tccattgtgg ggggaggtat
     1441 ttgcctgagc tgtctgatga aggcggcaat gaatggcgac acctttgttg taaggggtgg
     1501 gtcatgaaca gcgtcagttt tagccttgcg gcacaacacc tgatttgttc aacaaaagtg
     1561 acattatttg ctctggtgga tggtctgcaa tatgagcggt atttcggtga gagtctgtct
     1621 gttcaacaac ctgccgccgt gcccctgttc gatacctggc ctgattcgcg tattgcattt
     1681 gcggggccat gggttatgga gatgaacagc ataatggatt tcagagaaag gctgtgtgag
     1741 cttgaagctg cgcttccgtc agtttcctgg atgatatctt cttcctcgct tactgaactg
     1801 gctgctcatt tcagaaggaa tatgaataca gaactaccgg atggacggat tgcgttgctg
     1861 cgtttccatg atccgcgcgt tcagaagcgc ctgggagaaa tgctgaacga ccaacaacat
     1921 cgggagctga cggggttaat gcaggagtgg ctgacaatag tggacggaaa agcctggtca
     1981 tttaaacaaa gggagttcat atgctgaagt tgtctgaaga gcagtgtgtt ggtatggaca
     2041 aaagggaaga tgatgatttt gttcgcgaca ccgcgctaag actgaagaag aaatttccgg
     2101 gaattactga atcagatgac acgttccatg ttcgcctgag aaaagcgctg gattatgcca
     2161 atacccttcc gcttaaagag aaaaacgtga agcgggactt cttgtttctg gaggccttct
     2221 ggcctggttt ttatctgaaa cccgaagtgg ataaatggtt gagaacgccg aatgcttatt
     2281 caactgacca gcgggttgaa gattataagc atgtgatgat aaacagagaa aggagggagc
     2341 tctgatggtt atggtcctga caggggctgg tatggggagc gcagctaccg gaggtgttgg
     2401 tatggctggt gcctcaaccg gaacagcagt atttcccgga gcaggaagcc gcgatggaga
     2461 ctgggaagaa gataatatat cttatcctgg ctcaggcagt ggttcgcgtc agggtaacgg
     2521 agggttttat tatcagggga aacagtatga tccaattctg aatgagccga tggttgtcag
     2581 cgcagagacg catggtgatt tctgggaaga tgatatggat cattcagaaa cagtaaacta
     2641 tgaaggtatt gatagcaaag ccgaagtaca ggcgaagacg gatgagaaaa cgtgtgagaa
     2701 ctgcccaccg gaagggaagg taatgccgat gataaggcgt tgctcgcgct ggagtatggt
     2761 aacaatcagc taccagacaa gaatatgtgg cacgttttat aatccggaga cgcaacagat
     2821 acaggaattt aagtactgcg gcgtatcgtt tgatggctgg aaagataagc tgtgtcagtt
     2881 ctgggaagct aaagcgcggt atgaccaatt tttcagggct gatggttcaa agaaaccctg
     2941 gtggacaggt aatctaagcg caattaatca agcatcccga catcaggcgg ttgcaacagt
     3001 caatcagcca ttgaaagttg tctggatatt tatgcaacct gtaagttgtg agtatttcag
     3061 gaaaatgttt aaagattaca aggatattat tacgaggtgg caaccgtgat tgaattattt
     3121 aacacgtcaa taaatataca ttctttgaaa gaaagagaca taacgataga cgattgtttg
     3181 aatgaactat acagaagtac cgtagttgtt aaaaatctaa cgcagcacga tcacatatgg
     3241 tacttaactg gctactcaag aaaggacgct tcaaagcatg tcgtgtttag tgataatgaa
     3301 ccgaaccaaa atacaataga tacctttgag cggcattata aaaagaatcg tcctttaatt
     3361 aatgaatcta tatggaatgg acaacccgat gggctggctt gctcaatatc acattttatg
     3421 aaatttattg ataaccctaa aaaac
//
LOCUS       DAZGHF010000263         3411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGHF010000263 DAZGHF010000000
VERSION     DAZGHF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.265"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..609)
                     /gene="dsbA"
                     /locus_tag="ACHK3A_004982"
     CDS             complement(<1..609)
                     /gene="dsbA"
                     /locus_tag="ACHK3A_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO8742920.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKY"
     gene            complement(626..1612)
                     /gene="srkA"
                     /locus_tag="ACHK3A_004983"
     CDS             complement(626..1612)
                     /gene="srkA"
                     /locus_tag="ACHK3A_004983"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO8742921.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTPDQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1689..1958)
                     /locus_tag="ACHK3A_004984"
     CDS             complement(1689..1958)
                     /locus_tag="ACHK3A_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO8742922.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2028..2612
                     /gene="mobA"
                     /locus_tag="ACHK3A_004985"
     CDS             2028..2612
                     /gene="mobA"
                     /locus_tag="ACHK3A_004985"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO8742923.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWIHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            2594..3121
                     /gene="mobB"
                     /locus_tag="ACHK3A_004986"
     CDS             2594..3121
                     /gene="mobB"
                     /locus_tag="ACHK3A_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO8742924.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPVLCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALMDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(3224..3339)
                     /gene="rrf"
                     /locus_tag="ACHK3A_004987"
     rRNA            complement(3224..3339)
                     /gene="rrf"
                     /locus_tag="ACHK3A_004987"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          886 a          838 c          824 g          863 t
ORIGIN      
        1 atatttcaca gtatcagcat actgctgaac aaaaacatcc atattgctgg tatccatacc
       61 ctgcggattc agctgatatt taccgttaac aaacatcgcc ggaacgccac gcaattgcac
      121 gtcagctgca gctttttcct gctgagcgac cagggatttc accacgaagc tgttccacgc
      181 tgcgtcgtac tcttcacctt taatacctgc gttgataaat acatcgcgga tatcagatgc
      241 tgaacgaatg gtctgggttt tctgtacgcc ttcaaacagc ggaacagtga ctttgtcttc
      301 cacgcccagt gccatcgcca ctgcccatgc ctgagtcaga tctttgccca ggtcaccccc
      361 catgaagttg acgtggtatt tagtcatctt cacgccttcc ggcagttttt tcttcacgtt
      421 atcagaaata tgcagaactt cttcaaactg atagcagtgc gggcagaaga aagagaaaaa
      481 ctccagcact tgcggcgcgc cagcaactgg tttttccagg gtagtgtact gtttaccatc
      541 ttcatactgc gccgccgatg cgctaaacgc taaaactaaa ccagccagcg ccagccaaat
      601 ctttttcatg atctactctc tccgattaat acataggtgt taattgcaaa gggggttctt
      661 gtagaacttt tgcctgttct ataaaagtcg ccgtctgtcg cagccagtaa tcttccccgg
      721 ttaaccacgg gaaatttttc gggaacgcgg gatcagccca acgccgcatt agccaggcaa
      781 gataataaac caaacgcatg gcgcgtaaag gttcaatcag tccgatttca gcggtgtcga
      841 actcgctaaa ttcttcataa gcttcaataa tagtttccag ttgcatccgc tgctcggctt
      901 tatcgccatt gagcaacatc cacaaatcct gaatagccgg accattacgt gcatcatcca
      961 gatcaacaaa cattggacca tcgcgccaga gaatattccc ggcgtggcag tctccatgta
     1021 gccgcagaac ggtgaaatct tcccgccagt gtgctgtaac ggcggcgatc agctcatctg
     1081 tcgctttcag aaatgccgct ttcaacccgg aaggtatcag tgtagcgtcc tcaaacagct
     1141 tgcgaggctc aatgagatat tcattcagac cgatggtcgg gcgatggata aaaagctgtt
     1201 tgcgccccgt ctggtgcata cgccctaaat aacgcccaac cgcttccatc tgatcgatat
     1261 tatcagcttc gaactggcga ccaccgacgc ttggaaaaac agcgaaataa aatccctgat
     1321 gattcaataa agtctgaccg ttaaaggcca caggtgctgc gaccggaact tcatcattta
     1381 ccaactgcaa cgcaaattga tgttcttcga ggatttgatc gggtgtccaa cgttcaggac
     1441 gataaaattt gacgacaaaa cgtcgacgat cttcgtcctg aaattgatag acacggtttt
     1501 catagctgtt aagcggggta agaccggaat ccacccggat cccttgctca aacagagcat
     1561 ccatgatggt atccgggtgt agtgtctgga aagtaaaagc gctgttgttc atcccatcat
     1621 ccggaaaaca ttacgaatga tgaaggatat catttcacga tgattttggt ggcaccgctt
     1681 acaagctttt actctttaat tacgccgcga gcgcgtaaca gcgccgtttt gaagtcttct
     1741 tcataatctt tctgcaaacc cgggatcacc gcatctttcg cggaatcacg cattttcagg
     1801 tgatagatca gaatgtcatc cgtcaaatcc gccagttcgc cgtcaaaacc tgactctttc
     1861 gccagtttct gcaaaaattg cagcaggtta aggtctggct ctttttgcca ggctggctgg
     1921 aggagttcaa taacttcatt cagacgttta catttcatag taatgctcct tactcttgag
     1981 acagacacgt tagcagggtc aatcccacaa taaaagaggc gatatcggtg aatctgatga
     2041 cgacgataac aggcgttgtg ctggcaggcg gtaaagccag acgaatgggc ggcgtagata
     2101 aaggattgct tgaattaaac ggcaaaccat tatggcaaca tgtcgctgac gcgcttatga
     2161 cgcagctctc tcacgtcgtg gttaatgcta atcgtcatca ggaaatctat caggcaagcg
     2221 gtctgaaagt gattgaagat tcactggcgg attacccagg ccctctggca ggaatgcttt
     2281 cagtaatgca gcaggaagcg ggtgagtggt ttttgttttg tccgtgcgat acgccttaca
     2341 ttccccatga tttagccgcc cggcttaatc atcagcgcaa agatgcgcct gtcgtgtgga
     2401 tccacgacgg tgaacgcgat cacccgacta ttgctctggt aaaccgcgca attgagcctt
     2461 tattactgga atatctgcaa gcaggagaac gccgggttat ggcatttatg cgtctggctg
     2521 gcggtcatgc ggttgatttc agcgatcgta aagaggcatt tataaacgtg aatacgccag
     2581 aggagcttgc ccgatggcag gaaaaacgat gataccgtta ctcgcctttg ccgcgtggag
     2641 tggtactgga aaaactacac tgctgaaaaa actgatcccg gtattatgcg ccagagggat
     2701 ccgtccggga ctgattaagc atacgcacca tgatatggat gttgataaac cgggcaaaga
     2761 tagctatgag ttgcgcaagg ctggcgcggc acaaaccatc gttgccagcc agcaacgatg
     2821 ggccttgatg acggaaacac cagacgaaga agagctggat ctacattttc tcgcaagtcg
     2881 aatggatacc tcaaagctgg atttgattct ggtcgaaggg tttaagcatg aagagatcgc
     2941 aaagattgtg ctgtttcgcg atggagccgg acatcgaccg gaagaattag tgatagacag
     3001 acatgttatt gctgtagcca gtgatgtgcc gcttaatctt gatgtcgcgt taatggatat
     3061 taatgatgtt gaggggctgg ctgattttgt ggtggagtgg atgcaaaagc aggacggata
     3121 agcaaaagta ggctggataa gacgcgccag cgtcgcatcc ggtatttatt ctgctccagc
     3181 gcaaaaaggc catcctgacg gatagccttc tgcttaattt gatgcctggc agttccctac
     3241 tctcgcatgg ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg
     3301 catggggtca ggtgggacca ccgcgctaag gccgccaggc aaattctgtt ttatcaacac
     3361 gtctcgttat gacatgtcga tttaatctgt atcaggctga aaatcttctc t
//
LOCUS       DAZGHF010000264         3406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGHF010000264 DAZGHF010000000
VERSION     DAZGHF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.266"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..278)
                     /gene="ybgE"
                     /locus_tag="ACHK3A_004988"
     CDS             complement(<1..278)
                     /gene="ybgE"
                     /locus_tag="ACHK3A_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO8742925.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGL"
     gene            complement(278..391)
                     /gene="cydX"
                     /locus_tag="ACHK3A_004989"
     CDS             complement(278..391)
                     /gene="cydX"
                     /locus_tag="ACHK3A_004989"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO8742926.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(406..1545)
                     /gene="cydB"
                     /locus_tag="ACHK3A_004990"
     CDS             complement(406..1545)
                     /gene="cydB"
                     /locus_tag="ACHK3A_004990"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO8742927.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFHLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(1561..3129)
                     /gene="cydA"
                     /locus_tag="ACHK3A_004991"
     CDS             complement(1561..3129)
                     /gene="cydA"
                     /locus_tag="ACHK3A_004991"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO8742928.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT          958 a          931 c          829 g          688 t
ORIGIN      
        1 atcagcccga caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga
       61 accttctgcg gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc
      121 aataaaccat gccagatttc cagttcactg gtcttcgcgg cgaaacgtga tgggtcccaa
      181 aacatacatc ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg
      241 tccattaccg catacaaggt tgcgataatc ttactcatca gatgtcttct tgaccggctt
      301 tgcctgattc gacgtgctca agcgccagcg cggtgattac cccaaacgaa caggcaagaa
      361 gcgttcccag aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg
      421 ggtgttacgt tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc
      481 ggtgtagagc agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt
      541 cagctggctg gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg
      601 catcacaaac gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga
      661 ggagaacaca aacgcccacg ccgctttatc catacgtgca gtcaggatgg tcagcagcgg
      721 cagaaccaca cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag
      781 ccatgcgcca gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc
      841 cattgtcgat ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc
      901 gaaacagacc agtgtcacca gtgccgccac ctgagccgtt gcacgggtac gcaggtgcag
      961 ttcgcccacg gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct
     1021 caccacgcct gccagcaggc cgaacgggtt aagcaggtgg aagaagttac cggtgtagta
     1081 cagacgcaga tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac
     1141 accaatcacc aacggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg
     1201 ccagcgggtt tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga
     1261 cgccagcacg aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg
     1321 ccaggcagca aagagtgcgc cgcccgcggt gataagccat acctggttac cgtcccagtg
     1381 tggtgcaatg gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgagt
     1441 gagcatgccc acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc
     1501 aaccagcagc caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc
     1561 ttagcgtgcc ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag
     1621 gctgcttggg ccgaggcgtg caaatttgaa cattaagaac aattctgcca ccaggaacag
     1681 ggtatacagg ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga
     1741 gttcgccaca gctgtcggca gcacttcacc gatagcccac ggttggcgac catattcagc
     1801 cacgaaccag cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg
     1861 cagcagccat tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat
     1921 gattgccagc agcaggaagc cacacgccac catgatacgg aacgcgaagt acagcggcgc
     1981 tacacgcgga atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac
     2041 gtttggcgta tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa
     2101 ctggtcacga accgcctggt cggtagaacc agaacgcagt tgctcgagca gagagtacgc
     2161 cttcatcccg ttacgaatgc gttcttcatg ctgcaccatc agttctttca ggccgataac
     2221 tggagtatcc acggaacgcg ttgcaatgat gcccagcgcg taagggatct ggatcgcaaa
     2281 tttgttcgtc tcctcttcct gatcaggaat gccgaacaga gtaaaggcag caggcgcagg
     2341 ttgcgtttcc cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc
     2401 gtaaccagat tcatcaccca gaacaataac agacagaacg gcagccatac cgaagctggc
     2461 agcgatagca aaagagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc
     2521 gctgataccg aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa
     2581 tttcacctga gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat
     2641 agtttcaaag ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat
     2701 ccacagtgcg gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac
     2761 tttacccaga cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa
     2821 ggccatcaga ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata
     2881 gtaagaccag ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc
     2941 gaagttgata ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc
     3001 ggaaaggacg tagaccgttt ccataatggc cagcaggaac gccataccga gcgtcagtgg
     3061 cacaaaaagg aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat
     3121 atctaacatc atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa
     3181 agggtcacac gcatgcccca atacaaaatt atttgctcgc ctctatcgtt gccatttcat
     3241 caaaagagga aaagacaatg ataaaaaagt tacaaatgtg ttaatgaata aaccaaattg
     3301 atcccgtaat tatattacgc cgcaaaatcc ttacaataaa caggttttta ttgagcaaat
     3361 ttgcattttt cgacagtgat caatttatag cgaaattgtc acgatc
//
LOCUS       DAZGHF010000265         3386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGHF010000265 DAZGHF010000000
VERSION     DAZGHF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.267"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            690..1466
                     /locus_tag="ACHK3A_004992"
     CDS             690..1466
                     /locus_tag="ACHK3A_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8742929.1"
                     /translation="MKRSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPAAHLL
                     VLLGIFGYIMHRTMPGISFPVFLLNGLIPFFIFNSISNRSVEAIDANQGLFNYRPVKP
                     MDTIIARTLLETLIYIIVYVLLMLIVWMSGEYFEIISLLQLVLVWTLLIIISCSVGLI
                     FMVVGKTFPEMQKILPIFLKPLYFISCIMFPLHSIPQQYWTYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            1463..2134
                     /locus_tag="ACHK3A_004993"
     CDS             1463..2134
                     /locus_tag="ACHK3A_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8742930.1"
                     /translation="MIKIENLTKSYRTSTGRHYVFKDLNIELPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDRGKIITNKTISWPIGLAGGFQGSLTGRENVKFVARLYAKPEELK
                     DKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAHYFKERHKESSFLMVSHSLNSLKEYCDVVIYIKSFNEVKYYSVVTDALAEYIID
                     TKIQV"
     gene            2160..3095
                     /locus_tag="ACHK3A_004994"
     CDS             2160..3095
                     /locus_tag="ACHK3A_004994"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO8742931.1"
                     /translation="MSIIVNHVMSSDIVSGIFTDLLSYYKRYCDRDISIVESSNAIDN
                     ADVYHYHRPHLESRLKENSVVTIHHDLYDTDPWLDYDKFHPRYMEAKKILCLNSSQQN
                     YLNNKGLMNTEIIPHGYNSDIFCSVGAQKKIKNKVCIGFISKRYGRKVKGEAYLYEIF
                     KRLDSKRYRFIFVGVDRTITAYKASRFGFEVKCYERLPYYCYGDLYKQLNFLLVTSLY
                     EGGPANIPEAIASGTPIISTPVGMVNDYVRDGINGLILTGDIDNDIDSINNFSQKSKY
                     QKLSSNALEMSGNIMSWQDVMQKTSLVYKELAGCA"
     gene            3096..>3386
                     /locus_tag="ACHK3A_004995"
     CDS             3096..>3386
                     /locus_tag="ACHK3A_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFO8742932.1"
                     /translation="MKNISILFADSIHFHVRNFKSLFDLIDRHKIHHTFIHERNKWVS
                     AYGNYEEFFNELQPLYNKLLGKDIEQLYSLTIYGINIFKVCRDELLSYYLPID"
BASE COUNT         1089 a          538 c          686 g         1073 t
ORIGIN      
        1 catatcttta agagtaagct attagatgta aattactttg gtcaggtcct tcacagcaga
       61 aacagaatgt catgaaagat aaatttacgc agtcacttga tcaactgctt agttttgcgt
      121 cttaaaggat tgtaactatg gcaactgtgg agcgaaaaat cattgggcac gggaaaagtg
      181 cggtcatata gaaaagtgct ctctattcag catctcatgg tttatcagcg aagacagaaa
      241 aaaattataa cgatgcgcag gaatttgtgg aggtttgcta atatagagcg attcgagcat
      301 ccctcgttaa atctctcgaa ccgcagtata ttcagacatt tttaaaatac gccagatgat
      361 tacgacagaa agaacacaac atataattca tgttcgctgt cctcttgctg aatacggaat
      421 tgtcttcaat aaaggtgcta cacattatgg tgagagctac cacggtttct tgaggatgca
      481 gaaaatggat ttaccgatat cgtgcaattt tgtatcggta gtatgtccga ctcatgtctc
      541 atggataatg aaccatatat tataactgcg ggtagaaaaa tgcgcatcta acgactttgc
      601 ctggacgtgt cgattaactg ttcctgggtt tgctactctt gtcagtcaga cgataaaagt
      661 gagcataaat atataggatt acatatcaag tgaaaagaag tggatttgaa gttcagaagg
      721 tgacagtaga ggcattattt ctacgagaaa tacgaacacg ttttggtaag tttcgtctgg
      781 gatatctgtg ggcaatcctg gaacccgctg ctcatttgct ggtgctgtta ggtatttttg
      841 gctatattat gcatcgtacg atgccaggta tctcgttccc ggtattttta cttaatggtt
      901 tgattccctt ttttatcttt aatagtatca gtaatcggtc tgtggaagct attgatgcaa
      961 accaggggct ttttaattat cggccagtaa agcccatgga tacaataata gccagaaccc
     1021 tgcttgagac actaatttac attatagttt atgtgttgct tatgcttatt gtctggatgt
     1081 caggagaata ttttgaaata ataagtttat tacaactggt acttgtatgg actcttctga
     1141 ttattatttc atgtagtgtc ggattaatat ttatggtcgt tggtaaaact ttccctgaaa
     1201 tgcaaaagat tctaccgata tttcttaaac cgctgtattt catttcctgt atcatgttcc
     1261 ctctacattc gatcccacag caatactgga catatctact ctggaaccca ttagtccatg
     1321 tcgtagagtt aagtcgtgag gcagttatgc cgggctatat cagcgaaggc gtgagtttga
     1381 actatctggc aatgttcact ctggtcaccc tgttcatcgg tctggcgcta taccgaacgc
     1441 gtgaagaggc aatgctgaca tcatgattaa gattgagaat ctgacgaagt catatcgcac
     1501 gtcaacaggt cgacactatg tgtttaagga tttaaatatc gaactccctt caggaaaaag
     1561 tgttgccttt attggtcgta atggtgcagg taaatcaact ttactgagaa tgattggcgg
     1621 tattgaccgc cccgatagag gaaagatcat caccaataaa acgatatcat ggcccattgg
     1681 gctcgccggt ggattccagg gcagtttaac cgggcgagaa aacgtaaaat ttgttgcacg
     1741 gttatatgcg aagccagaag aactgaaaga taaaattgag tttgtcgaag aatttgccga
     1801 actcggtaag tactttgata tgccgatcaa aacttactca tctggtatgc gatctcgcct
     1861 gggttttggt ttaagcatgg catttaagtt tgattattat attgtcgatg aagtaacggc
     1921 agtcggtgat gccaggttta aagaaaaatg tgcacattac tttaaagaaa ggcataaaga
     1981 gtctagtttt ttaatggttt ctcatagttt aaattctttg aaagagtatt gcgatgtggt
     2041 tatatatatt aaaagtttta atgaagtaaa atactacagt gtagtaactg atgcgctggc
     2101 agaatatatt atcgacacta aaatacaagt ataaactttc caagtaacaa ggcgttatta
     2161 tgtctataat tgtaaatcat gttatgtctt ctgatatagt aagtggaata tttacagatc
     2221 ttctctctta ttacaaaaga tattgtgata gagatatatc aattgttgaa agtagcaacg
     2281 ctattgataa tgctgatgtc tatcactatc atcggcctca tttagaaagc agacttaagg
     2341 aaaactcagt agtaacaata catcatgact tgtatgatac agacccatgg ttggattatg
     2401 ataagtttca tccccgttat atggaagcaa aaaaaatact gtgccttaac tcctctcagc
     2461 aaaattattt aaacaataaa ggcttaatga atactgaaat tataccacat ggttacaatt
     2521 ctgacatctt ttgctcagta ggggcgcaaa aaaaaataaa aaataaagtt tgcattggtt
     2581 ttatatcaaa gagatatgga cgaaaagtga aaggagaagc ttatctttat gagattttta
     2641 aaagattaga ttctaaaaga tacagattca tctttgtagg agtcgataga acaataacag
     2701 catataaagc atcaagattt ggttttgaag taaaatgtta tgaaaggctt ccctattatt
     2761 gttacggtga tttatataaa caacttaatt ttcttttagt aacgagtttg tacgaaggtg
     2821 gaccagcaaa tatacctgaa gcaattgcat ccggtacgcc aataatatca actcctgtgg
     2881 gtatggtaaa tgattatgtc agggatggta ttaatggtct tatattaact ggagatattg
     2941 ataatgatat cgactctata aacaatttct ctcaaaaaag taaatatcaa aaattatcaa
     3001 gtaatgctct tgaaatgtct gggaacataa tgtcttggca ggacgttatg caaaaaacat
     3061 cattggtata caaagagtta gcagggtgcg cttgaatgaa aaatataagt atattatttg
     3121 cggacagtat acattttcat gtgcgtaatt ttaaaagtct attcgattta atcgacaggc
     3181 ataaaattca ccatacgttc atacatgaac gaaataaatg ggtgagtgct tatggtaatt
     3241 atgaggaatt ctttaatgaa ttacagccat tatataataa attgctgggg aaagatattg
     3301 aacaattgta ttcacttaca atttatggaa ttaacatctt taaagtatgc cgggatgaat
     3361 tattatcgta ttatttgcca attgat
//
LOCUS       DAZGHF010000266         3359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGHF010000266 DAZGHF010000000
VERSION     DAZGHF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.268"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            137..1687
                     /locus_tag="ACHK3A_004996"
     CDS             137..1687
                     /locus_tag="ACHK3A_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO8742933.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANISGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGIRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            1728..3347
                     /locus_tag="ACHK3A_004997"
     CDS             1728..3347
                     /locus_tag="ACHK3A_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO8742934.1"
                     /translation="MKTAQRTLFAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLANVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTPKKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSDVVIDYLKKGHKITPAQVNEMRVDVSK"
BASE COUNT         1056 a          693 c          711 g          899 t
ORIGIN      
        1 aatgtttaat taataacatt atttgttact aagatcacac aatacttttc tgaactatgc
       61 ttacataaaa taagacacga tgtgtctttt atttaaataa ttcaacaaat gtaacacacc
      121 ctatccagga gggatgatga aaactgtatt tacaacatta tcagtcatat tcggcgtgat
      181 attttctcac agtgttctag ctcaggacgt tactatttat tatacgaatg acattcacgc
      241 acatgtcaat ccagcaaaaa tcatcgctgt tgataagaac agacttatag gtggtatggc
      301 taatatttca ggcatcgtta atgaagcgaa gaaaaaaagc aaagatgtgt ttttctttga
      361 tgcaggcgac tattttaccg ggccgtatat cagcaccctg accaaaggtg aggctattat
      421 tgatatcatg aacactatgc cctttgatgc ggtgtctgtt ggtaatcacg agttcgatca
      481 cggtgtgccc aatatggtgt ctcagttatc aaaagcaaaa ttccccattc tgctgggaaa
      541 tatctactac accgatacga ataaaccagt ttgggatcat ccgtggacca ttatcgaaaa
      601 agatggttta aagattggtg tgattggatt gcatggcgca ttcgctttct atgatacagt
      661 cgcggcaaaa gcacgtgaag gcgtagaagc cagagacgaa attaaatatt taaataaagc
      721 acttgcagaa ttaaaaggta aagtcgacat taccgttttg ctaattcacg aaggtgtccc
      781 ggcgcgtcaa tcaagctttg gtagtaaaga tgtggagcgg ctactacagg cagatattga
      841 gacagctaaa aaagtgaatg gtgttgatgt cttaattacc ggtcacgcac atgtcggaac
      901 accgcaacca ataaaagtta ataacacatt aattgtatct accgatgcat acggcaccaa
      961 catcggaaaa cttgtgcttg attacaatcc gaaaacgaag aaaattgaca gttataatgg
     1021 tgagttaatc accatctttg cagatcaatt taaacctgac accatcgttc aaaatactat
     1081 tgataaatgg agtgcaaagc ttaacaaaat aacacaggaa gttgtcggcc attctcccgt
     1141 agttttaaca cgtgagtatg gtagttcctc ttctaccggc aaccttattc ttgatgcaat
     1201 gatggaaaaa acacctgatg ccgttgccgg atttcaaaat agcggtggga ttcgagctga
     1261 ttttcctaaa ggcgatatca cactgggaga tgttattagc acattcccct ttaataatga
     1321 cctcatcgag atggatttga cgggccgcga tctcaaatca ttgatgacgc atgcaaccaa
     1381 tctaactaac ggtgtgttac aagtttctaa aagtgttgcg gttgtctatg acagcaaaaa
     1441 acccctcaac caacggttaa tctctttcac cattaacggc aaacccgtgg aagataatca
     1501 aacatatcgt attgccacgc actccttttg tgccagtggt ggtgacggtt ttgaagcatt
     1561 tttgaatgga aaaaatgtga agacgatacc gggaacaacc tcggcggaat ctatcatcga
     1621 ttatttcaaa aatcataaac ctgtcacccc agacttaact aaacgagtca tggacgtcgc
     1681 caaataaaat ctcatttatc agtcaattta tttaacaagg aatgcgaatg aaaacagcgc
     1741 agcgtacttt atttgccgca acattgctga ctattttttc gacctcagtt atggcgcagg
     1801 atgtcacgat tatttatacc aatgatcttc acgctcatgt ggattcctat aaagtaccgt
     1861 atgtcgcaga cggaaaacgc gacattggtg gctttgccaa tatatccacg ttagtaaaac
     1921 aagagaaagc caaaaataaa gccacctttt attttgatgc tggagactat ttcaccgggc
     1981 catatatcag tagcctgaca aagggcgaag caataattga tattatgaat accatgcctt
     2041 ttgacgcagt atcaatcggt aatcatgagt ttgaccatgg ctgggataat gccctccgac
     2101 aattaagcaa agcgaatttc cctgttttat tagccaatgt ttatcataaa gagagtgaga
     2161 aacccttctg gaacaagccc tacaccatcc tggaaaaaga tggcatcaag attggtattg
     2221 ttgggctaca cggcgtattt gcatttaatg acacggtctc ggagctctct ctccagggac
     2281 tcgataatga caataataac cgatttgata aatcttccgc gaccctcaag aatcagggga
     2341 ttgaagcacg tgatgaagtg aagtatttgc agcattatat cgatgagtta cgtgataagg
     2401 ttgacctcac cgtggcgctg gttcatgaag gcgtaccggc gcgtcagtcc agcattggca
     2461 atacagatgt tagacgcgct cttgataaag atattcaaac cgcaagcaag gtgaaaggat
     2521 tggatattct gattaccggg catgcccacg tcggaacgcc agaaccaatc aaagtcggca
     2581 acaccttaat cctttcgact gacagtggcg gtatcgatat tggtaaatta atccttgatg
     2641 tcaaccctac agctcgtact cataaagtga agagctttga gctgaaaacg gtttatgccg
     2701 atgaatggat acctgatcca accacacaaa aagttatcaa tggctggaat aaaaaactgg
     2761 cggatcttgt acgccaacca gtcggtgaat catcgattgc attaactcgt gcctacggtg
     2821 agtcatcgca acttggcaac ctgtttaccg atgcaatgct tgtcgctgca ccgactgcac
     2881 agattgcatt gataaactcc ggcagcttac gtgcggatat aaatgcaggc acgattacct
     2941 ttggcgacat taccagtaca ttccccttca agaatgaact tactgaaatg gatctcagcg
     3001 gtaaggatct gcgtaaccta ctggaacacg gggcatcgct taccaatggc atcctgcaaa
     3061 tgtcaaaagg cgcagaaatg cgctataccc cgaagaaacc agtaggtcag cgcatagtat
     3121 cttttaaaat taatggtgaa gagatcgttg atacgaatat ttaccatgtt gcaacaacga
     3181 ctttccttgc actggggggc gatggttttc tggcattcaa agaagggaaa aatgttcagg
     3241 tccgcgccgg aaataacatg tccgatgtcg tgatcgatta tttgaagaaa ggccacaaga
     3301 ttacgcctgc acaggtaaat gaaatgcggg tggacgttag caaataataa aaaaattat
//
LOCUS       DAZGHF010000267         3353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGHF010000267 DAZGHF010000000
VERSION     DAZGHF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.269"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..230
                     /locus_tag="ACHK3A_004998"
     CDS             <1..230
                     /locus_tag="ACHK3A_004998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742935.1"
                     /translation="KNNTLKPLSRQYSSRFLTDFLNKKTSFAGGWSHCVTPTTITSVF
                     SRAVVNHKIGTHAGGAGPHDFLFDESENGER"
     gene            521..715
                     /locus_tag="ACHK3A_004999"
     CDS             521..715
                     /locus_tag="ACHK3A_004999"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO8742936.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIEAWKKWNDALHYAK
                     GENIGWCIARAEYCKKTGRF"
     gene            1145..1372
                     /locus_tag="ACHK3A_005000"
     CDS             1145..1372
                     /locus_tag="ACHK3A_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742937.1"
                     /translation="MSIDVIKQIQSIQDKGIIIYSKYRANEFNRDDVYRESYSLIIEF
                     NHLIAEHAVHDEKLINHTVCILNELRSIAIN"
     gene            complement(2039..3037)
                     /locus_tag="ACHK3A_005001"
     CDS             complement(2039..3037)
                     /locus_tag="ACHK3A_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO8742938.1"
                     /translation="MKNPELKNLTDYSPVDKPWDEHRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTSMNAGFKRLKERKEFRHVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVAETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRRLSSDD"
BASE COUNT          939 a          832 c          754 g          828 t
ORIGIN      
        1 aaaaaaacaa cacactgaaa ccactctccc gacaatactc cagcaggttt ctcaccgact
       61 ttctgaacaa aaaaaccagc tttgcaggtg gttggtccca ctgcgtgaca ccaaccacca
      121 tcaccagtgt tttttctcgt gccgtcgtca atcacaaaat cggaacccac gccggaggcg
      181 cgggacccca cgattttctg tttgacgagt cagaaaacgg cgaacgctga cagatttcaa
      241 tctggctgcg tgagacgttg gcggggtgtc ggggcgcagc cctgaccagg cggcatttgt
      301 ctgattgcgt gtgcgcggtc cgacaaatac acatcctgtc ccgtttttcg cacggttttt
      361 attgattacc ggacagatga atccggattg ccatgttgcg aaaatcacgt taaaacgatt
      421 acgagcgtgc agttgggctg atatatcaac agtcatgcca atatgtatta acccgacagg
      481 ccgctctttt ttaattcaaa taaaaatata aattacgagt atgaatcagg acaaagcaat
      541 ttatttagaa aataaatatt tttcacccgc aaacgaagct gtcagactgg agcgtgaagg
      601 agattacatt gaagcatgga aaaaatggaa tgatgcgctt cattatgcca aaggagaaaa
      661 tataggttgg tgtatagcca gagctgaata ttgtaaaaag acaggccggt tttaattctg
      721 gcggggtttc aaaggggcgt cccctttggc acgtcgacgt atcaaaatgc gccagcattt
      781 tgtatacagt cggtcacgtg cctccaggat tgccccataa tatttcctat ctacataatg
      841 tctaactaag atccatctaa tatccatcta agatccattt aaggtccatc taaatcagta
      901 aatatctaac tagtgtccat ctaaggtcta tctaacgtcc atctaaaagc taactaacgg
      961 ttcaccaacg gatcgcatgt agtagacgcc acataaaaaa tcctgaaatg gaggaatcgt
     1021 cataaagaga ttcctgtttt tagcgttctg atgcaggata gggcaaacgc cgcaaaatga
     1081 cgtctttgat atattttgtg atgtttgttt cgggcatctg ttatctgttt tattgggtgt
     1141 ttttatgagt attgatgtga ttaaacaaat tcagagcata caggacaaag gaattattat
     1201 ttattcaaaa tacagagcaa atgaatttaa ccgggatgat gtatacaggg agagttacag
     1261 cctgattatt gagtttaatc atcttattgc tgaacatgcc gttcatgatg aaaaactgat
     1321 taatcatact gtctgtattc tgaatgaatt acgaagcata gcgataaatt aaaaaatccc
     1381 cctggattac agggggaagc catcagagtt tgagtaatga gaaatattat tttggagaga
     1441 gcaccacaaa tgcgctcttt tccaatctgc cgtaaccatc agcaaaagac aatcaggaca
     1501 ggcgaaaaaa tccccctata tggcaagggg gtgtcaacga ggacatggca attgtgagtt
     1561 ttgtgtgttt tatgtgttgc agtttgcaac gtcagaaatt tgccgcagtt atgcgcgaaa
     1621 aacaatcctg atttcgggac aggatgtgta atcgtataac cgcgcacgca cggctatacg
     1681 attaccacct ggtcagggct ttgcccgaca ccctcgacac cgttcccgca atacgataga
     1741 acatcagggc gcgtttccgg gtaaacgcat gttaatgacc gggaattcat tgattaggga
     1801 ccggagacgc gctacaaaac tcctgcgcgt ttctggcatc aactgaccat ggggatggtt
     1861 tggcaatagc agcaagcgcc agaaacgcgc tcaaaacgtc tctgacggcc tgaatcaacc
     1921 aaaacatata aaaccattgc ctgatgcaaa aaatcgcgtt agaaagcgtc tgagtgcgtt
     1981 tctggtgagc aaaaagctgt cagccgtgga ttgccattgc agagacccag aaacgcgctc
     2041 aatcatcgct gcttagccga cgtttatctt tcgcccgatc gcgctggtat tttttgacaa
     2101 ccggcaccca cgaaaacgcc agacgggaac cgtcatctgt accttctgcc atgccatccg
     2161 ccatgattaa atcctcgttc gtttcccgct ccagttgcag aacgttctta agcgctcccc
     2221 ccgtggcaac aaaacgacgc ttgtgtacct gccgtgttaa ttcgaggaac cattcaggat
     2281 cagccagcat atcagcaggc tttgtggaat atttcagagt ttcagccacc gcgccgcgca
     2341 gctgttccgc aacaacacaa ggtaaagatt catcgtcttt tggtttccgt gacttcacca
     2401 ctctgatatc cacattcggg tcatacgcca cccgcagaca atcacgccac agttccaccc
     2461 aacgggcatg tttcacatac tcacggccgg acaacatcga ggggggaacc atcagcagag
     2521 tgtgaaaatg gggatgtgcg ctaccatcct tgccgcgcgt tacttccgtg gtacgaaccc
     2581 agcccaacac atggcgaaat tccttacgct ctttcaaccg cttaaacccc gcattcatcg
     2641 aggtaagcgt ctcaccaagc gcgtcaatgg aacagttgcg cactgtcagt gtgagaaaca
     2701 accagcgcgc gccgggatgt tccgtcacaa tctttggcag ggactgatag aaacgggctt
     2761 gccacatcat ggaacgcctc cactgacaga tcggacagtg ccggacgcga cagaaccgtg
     2821 catcacgcaa ccgcaggcgc gtttctccgg tctctgtgtt tgttgaccac ccaaaccgca
     2881 aaataccact acaagccccg acgcgttcgc cgtagtgctc aaatccggca gcacgcagat
     2941 aaataccgcc cacatcgtcg gaaaagcttc ggtgttcatc ccacggttta tctactggag
     3001 agtaatccgt cagattctta agttcaggat ttttcatgtc tgccgtcccc ttgcgagcga
     3061 cagacgaaat tgcgtgttat cattcctgta tcacctgtac caattgtcgt caaatcagtt
     3121 catgttctag gtggtcgcgc ccctggagtg gtggaagact cgcaggggct aatttataac
     3181 cactcccgac aaaatactct caaacctatt tacacgcaaa tccgcgctca ggccagatat
     3241 ggcaagggat agaggcgatc caaaaaatcg catttatccc ttaagtataa atacaaggat
     3301 cgcgcgcacg atcacccacc caaaatgcgc gttttttgag cgtttccgag cac
//
LOCUS       DAZGHF010000268         3237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGHF010000268 DAZGHF010000000
VERSION     DAZGHF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.270"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            151..1833
                     /gene="ydeN"
                     /locus_tag="ACHK3A_005002"
     CDS             151..1833
                     /gene="ydeN"
                     /locus_tag="ACHK3A_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8742939.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTYSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEKLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1885..3042
                     /gene="ydeM"
                     /locus_tag="ACHK3A_005003"
     CDS             1885..3042
                     /gene="ydeM"
                     /locus_tag="ACHK3A_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO8742940.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHTENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETISYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3008..3130)
                     /locus_tag="ACHK3A_005004"
     CDS             complement(3008..3130)
                     /locus_tag="ACHK3A_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742941.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
BASE COUNT          984 a          700 c          649 g          904 t
ORIGIN      
        1 atagaaagta atgtcaacag tttatggact gttatttctc tttttttagc ttaatctgct
       61 atctctttat gtgactaact tcacttacat ccacttattt ctcttcgtaa aattactttg
      121 gaattaagta caataagaag aggaacattt atgaagtctg cattaaagaa aagtgtcgta
      181 agtacctcga tatctttgat actggcatct ggtatggctg catttgctgc tcatgcggca
      241 gatgatgtaa agctgaaagc aaccaaaaca aacgttgctt tctcagactt tacgccgact
      301 gaatacagta ccaagggaaa gccaaatatt atcgtactga ctatggatga tcttggttat
      361 ggacaacttc cttttgataa gggatctttt gacccaaaaa caatggaaaa tcgtgaagtt
      421 gtcgatacct acaaaatagg gatagataaa gccattgaag ctgcgcaaaa atcaacgccg
      481 acgctcctgt cattaatgga tgaaggcgta cgttttacta acggctatgt ggcgcacggt
      541 gtttccggcc cctcccgcgc cgcaataatg accggtcgag cgcccgcccg ctttggtgtc
      601 tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttcct gcctgaatta
      661 ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc aaaaatcagt
      721 aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt caccacctat
      781 tctgcggaag aatggcaacc gcaaaaccgt ggctttgatt actttatggg attccacgct
      841 gcaggaacgg catattacaa ctccccttca ctgttcaaaa accgtgaacg cgtccccgca
      901 aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga tcgtgccaaa
      961 acacttgacc agccttttat gctttacctg gcttataatg ctccgcatct gccaaatgat
     1021 aatcctgcac cggagcaata tcagaagcaa tttaataccg gtagtcaaac agcagataac
     1081 tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga aaaactgaag
     1141 aaaaacggac agtatgacaa tactattatt ctctttacct ccgataatgg tgcggttatc
     1201 gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac ctatcctggc
     1261 ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg taattatgac
     1321 aagctgattt ccgcaatgga tttctacccg acagctcttg atgcagccga tatcagcatt
     1381 ccaaaagacc ttaagctgga tggcgtttcc ttgctgccct ggttgcaaga taagaaacaa
     1441 ggcgagccac ataaaaatct gacctggata acctcttatt ctcactggtt tgatgaggaa
     1501 aatattccat tctgggataa ttaccacaaa tttgtccgcc atcagtcaga cgattacccg
     1561 cataacccca acaccgagga cttaagccaa ttctcttata cggtgagaaa taacgattat
     1621 tcgcttgtct atacagtaga aaataatcag ttaggtctct acaaactgac ggatctacag
     1681 caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca aggcgtggta
     1741 agagagttta tcgacaacag ccagccaccg cttagcgagg taaatcagga gaagtttaac
     1801 aatatcaaga aagcactaag cgaagcgaaa taactaaacc ttcatgcggc ggatttttcc
     1861 gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag ttttcaatgt
     1921 aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac tcatgaaaaa
     1981 tggatggatg acagcactct gaaagagttc atcaaacaat atatcgcagc gtctggcaat
     2041 caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga ttttttccgt
     2101 aaagttattc actatcaaca acgctatgca ggccaaaaac gtatttttaa tgcattacaa
     2161 acgaatggca ttttattgaa taatgaatgg tgtgcctttc tcaaagaaca tgagtttctg
     2221 gttggtatct cgatcgatgg cccccaagag ctacatgacc gttacagacg cagtaattca
     2281 ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc atatcaaata
     2341 gagtttaata cgttaaccgt tattaataac attaatgtcc attaccctct tgaggtttat
     2401 cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct cgaaaccggg
     2461 acgccgaata ttgatttcag tggtcatact gagaatacat tccgtatcat tgatttttct
     2521 gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg ggttaaaaac
     2581 gatgtgggtg aaattttcat ccgtcagttt gaaagctttg tcagccgttt tttggggaat
     2641 gggcatacca gttgtatttt ccaggagtcc tgcaaggata atctggttgt tgaaagtaat
     2701 ggagacattt acgaatgcga ccattttgtc tatccacagt acaaaattgg aaacattaat
     2761 aaatcggaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa acggatttca
     2821 gcgaaatgtc agcaatgtgt atataaacct atctgcaatg gtggttgccc taagcatcgt
     2881 attactaaag taaacaatga gactatttcc tatttttgcg aaggttataa aatccttttt
     2941 tcaaccatgg taccttatat gaacgccatg gtggagttag ccaagaacag agtaccgctt
     3001 taccacatta tggatgttgc aaaacaaatg gagaataatt aataacgctc cttctttgct
     3061 ccagtcttag cctccgtctt atgaccttaa caaagcaccc gacataaatt cctgttgggt
     3121 gtttcgtcat catcatcttc ccttgcgaaa attctggctt acgcggcatg cgtcagggca
     3181 ttagcaatac ccttgattat caatagcata accataacca ctgaaaatga aatattt
//
LOCUS       DAZGHF010000269         3214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGHF010000269 DAZGHF010000000
VERSION     DAZGHF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.271"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            77..568
                     /locus_tag="ACHK3A_005005"
     CDS             77..568
                     /locus_tag="ACHK3A_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8742942.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     ASSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            571..1839
                     /locus_tag="ACHK3A_005006"
     CDS             571..1839
                     /locus_tag="ACHK3A_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFO8742943.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMAVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGFILIPGK
                     GWRLSEAYDLNPVVRSDGLKLNITENDNALDLELAREVAEYFRLSLTEADDIIANFRG
                     IVSQWRIIAERLRLPGREQELMAEAFRASI"
     gene            complement(1982..>3214)
                     /locus_tag="ACHK3A_005007"
     CDS             complement(1982..>3214)
                     /locus_tag="ACHK3A_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8742944.1"
                     /translation="CFITVINYLDRTALGIAAPTIMETTGITKEQYSWIVSAFQLAYT
                     LGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLAFMRALMGFSEASAIP
                     AGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMFHSWQFAFIVSGSLAL
                     LAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKTSIKHILSQRNFWGIG
                     IARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLGDLGCLASGFVAKFFH
                     DRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIGAFSHQCLSTVAATLG
                     GDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPFFIALAFFDIIGAIAL
                     WMLIKVKDEEPQVQLATS"
BASE COUNT          928 a          661 c          775 g          850 t
ORIGIN      
        1 ctttgcaggt gcgttcaatc caataaatgc taatattatg gctatcatag ccataaaaag
       61 ataaaatatt atcactatgg gtaaaaaaac agcaccgtta ctcccttata cgaatcagat
      121 gcttagtgaa tttggtgagc ggctgaaact tgctcgttta cgacgcaggc ttaccgcaaa
      181 gcaagttgct gaacgtgctg ggatgtcgcc aataacattg cgctctctgg agtcaggaag
      241 tgcaggggtc acgatagggg catatttgtc tgtgatgcag gttttgggcc tggaaacgga
      301 tttaaataag cttgcagctg aagacgactt agggcgtcag ctccaggatt cccgtttgat
      361 taaaaaaaga gggctttctc gtgcatcaag taagaatgaa gaaggttctc agacagcctc
      421 attgcagatg aggcacgaga aagaaggtca tctcaccacg gatttaacag cactggcaga
      481 aggctcgacg ccatctatta caggagaaac atcaacctct cttgcagcac tattaaaacc
      541 gataaaaaaa ccgttaaaag gaaattaatt atggcattaa tcggagtgta tgcggattgg
      601 gaaggcctgg atggtccgga gcggattggt tatttacaca gtcgccgcac tcggacgcgt
      661 gagatatttg agtttgagta cgataaaaag gcgcttgcag atccttcact taatttcatt
      721 cagttagatc ctgaaataat gttgtatgag ggagcacagt acccaattcc tccaaaggat
      781 aaatttggtg catttagtga ttcctgcccg gatcgttggg ggcgtatgct aatgaagcgt
      841 cgttttgaga gggatattcg agacgggtta tgtgataaag actcccacct ttatgagtct
      901 gattatctac taggtgttca tgatctttat cgggttgggg cccttcgata caaacgtgaa
      961 gacgctggag agtttcttga taatcgcatt gatgttgcag ccccaccatt taccgaaata
     1021 gcgagtctgg aaagggctag ccgggcaatt gaagaagatc ctgataataa agagctaatg
     1081 gggcaaaaat ggttaagaat gctaatcgct ccggggggat cattaggggg ggcacgtccg
     1141 aaagccagtg ttgttgatga agctgggcat ctctacatcg ctaaatttcc cagtgtaaaa
     1201 gatgagtatg atgttggtgg atgggaaatg gccgttaatg cacttgctgt tggctgtgga
     1261 ctgaatgttg cacctgctca ggcgcataaa tttgccagca attaccattg ttttatggtt
     1321 cgtcgttttg accgcactaa tgcgggaagg cgtctgcatt ttgcttcggc tatgacacta
     1381 accaggcatc aggatggtga agatgcctct acaggtgtga gttatctgga attagcagat
     1441 gttcttatca ggcatggcgc gcagactaat actgatctta aggaactctg gtccaggatt
     1501 gtttttaata ttcttgtttc taataccgat gatcatttac gcaatcatgg atttatcttg
     1561 ataccaggaa aaggttggcg gctttctgag gcatatgatt tgaatcctgt ggtcaggtct
     1621 gatggtttaa aactcaatat tacggaaaat gacaatgcac ttgatttgga attagctaga
     1681 gaagttgcag agtattttcg acttagtttg acggaagctg atgacattat tgctaatttt
     1741 agagggattg tcagtcagtg gcgaatcatt gccgagcgtc tgagattacc tggtcgtgag
     1801 caagagttaa tggcagaggc attcagagcg tcaatttaga atagttattt cgatatattc
     1861 gctaaatcat aaatttgcga agcaatgcca gtagatactg aacgttttgg aagagacgtt
     1921 taatttatga gaaaaataga aacgctacaa agctaaatta tagcgtttct atgtaaaata
     1981 attagcttgt cgctaactgt acttgtggtt cttcgtcttt aactttgata agcatccaca
     2041 gcgcaatggc gccaataatg tcaaagaaag ccagggcaat aaagaacggc gcgaagccaa
     2101 taatgtgaac gaatgccccg ataaacaggt tgaaaatcaa ctgaccgctc catgcacagg
     2161 ctcctgccat acccacagcg gtagctactt cgtctttttt gaataggtca ccgcccagag
     2221 ttgctgcaac agtagaaaga cattgatgcg agaatgcgcc aatgctaatc agtaatacgg
     2281 cgatgtaggg attttcgaca atactcacca gaccaatcgt catcataatg acggctgcaa
     2341 tagtgaaggt aattcttcgt gagttaatta aactcacgcc gcgatcgtgg aagaacttcg
     2401 cgacaaaacc actggctaaa cagccgagat cgcccagcag gaaaggtagc cagacgaaca
     2461 tggcaatttc tttcaggcta aaatgcagcg tttcgacgaa gaaaatcggt acccagaagt
     2521 taatggttcc ccatgccggg tcagcgagaa aacgcgcgat gccaatcccc cagaaattac
     2581 gttggctgag gatatgcttg attgacgttt tttctttctt atctgtttta agatgctgtt
     2641 cttgcccaga ctcaatatag tggcgctctt catcagaaag gcgtttagca tcttttggat
     2701 ctttataaca aaagaaccaa aataaagccg cgagtaaagc aaggctacct gagacaataa
     2761 acgcaaattg ccagctatga aacataatgc accaggcaat caacggtgga gcaagcatcg
     2821 cgccgagtga ggtgcccata ttgaaaacac ctgtcgccac gccacgctct tttgccggga
     2881 accatgttga tgcggttttt acacccgcag gaattgccga tgcttcgctg aaacccatca
     2941 gggcgcgcat aaatgccaga cctgaccatg ttcctgtcaa ggcatgcccc attgtcgcca
     3001 ggccccagat tgcggcacat atcgcaaaac ttaacttcag acccacggta tcaataaaga
     3061 agcccattat cggttgcccc aatgtatagg ccaactggaa tgcactgaca atccatgaat
     3121 attgttcttt ggttattccg gttgtctcca taatcgttgg agccgcaata cctaacgccg
     3181 ttctgtccag ataattgatt acagtgataa agca
//
LOCUS       DAZGHF010000270         3043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGHF010000270 DAZGHF010000000
VERSION     DAZGHF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.272"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(161..>3043)
                     /locus_tag="ACHK3A_005008"
     CDS             complement(161..>3043)
                     /locus_tag="ACHK3A_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8742945.1"
                     /translation="IVGLQVDAEQFGGQQLTVNYHIRGRIIQVPSNYDPEKRTYSGIW
                     DGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGG
                     TEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDV
                     VVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDGILRYGRNLLKMD
                     AFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTG
                     GRILSIDAASRTLTLDREVILLEAGTSMVNLINGSGKPMRVDITAHPAPDRIQVSALP
                     DGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPM
                     SGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSGNGENSRLV
                     TTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAAPASIELTPGY
                     YQITAVPHLTVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGRQWSVSGPHIKPGKD
                     FWFYVRSVNLVGKSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQLDD
                     EMAEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKN
                     GIPYVAGIGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFL
                     KRLFAVSITSSGEPPTFSLTPDGKLTARNADISGNINANSGTLNNVVIAENCTIKGTL
                     RAENIIGDVVKTHNVSLPALSAAGDHRHATERTVTVIDDQSFDRNIVIPPVFYSGIVY
                     QGSDGDGATSICTLTVSVNDRVVFSKSSSIGTGRTSGGYPVQKDTMYPGSSTPFSYFM
                     RMASGGGDVRIKFRIEVIYYGTAPGFMTTGIQITTMKATASGFMES"
BASE COUNT          599 a          943 c          789 g          712 t
ORIGIN      
        1 atttgctgaa gatcgaaagt caggcctcgg gttaaacatg aatgtttgtc cggggccttc
       61 gtctttccgg ccttcaggtg ttccctccgg ccatcagcca aaggcacccg cgcatactgt
      121 acggtttttg tctccttccg gcaatcacga tcgtccggga ttaactttcc ataaatcctg
      181 aggctgtcgc cttcatcgtt gtgatctgta tccctgtggt cataaaaccc ggtgcagttc
      241 cataatagat gacctcaatc cggaacttga tcctgacatc tcctccccct gacgccatcc
      301 tcatgaaata gctgaacgga gtggatgacc cgggatacat ggtgtctttc tgaacggggt
      361 atccacctga cgtacgcccg gtcccgatgg atgagctttt gctgaacact acccgatcat
      421 tcacacttac cgtcagcgta cagattgaag ttgcaccatc accatcactt ccctggtaga
      481 caataccgga ataaaaaacc ggcgggataa caatgttccg gtcaaacgac tgatcatcaa
      541 tgacagtaac cgttcgttct gtcgcgtgtc tgtgatcccc tgctgcactg agagcaggaa
      601 gactgacatt gtgcgttttc accacatcgc caatgatatt ttccgctctc agagtacctt
      661 tgatggtaca gttctccgct atcacgacat tattgagcgt gcccgagttc gcattaatat
      721 tcccgctgat atccgcatta cgggctgtca gtttcccgtc cggcgtcagg gaaaacgtcg
      781 gaggctcacc tgatgaggta atactcaccg caaacagtcg tttcaggaac acatcgttca
      841 tgaacagctg attcccctgc gccacaaaca gcggcgtggt gttgccgttc tccgggttaa
      901 tcatcgcgat acggtccgcc tgcagcagta tattgctcag gggctggcca tcagcatcct
      961 caatccccgc acctataccg gcaacatacg gaatgccgtt tttcgttttc tgcaccttca
     1021 gcatgtacag cgcagccagg tcgttatttg tgtctgtctg cacccgctgt atctgctgta
     1081 tggtggcact ctggtcttcc agggttttac tgaccgtctg cgtgatttca ttgcgggttt
     1141 ctgtgatggt ggtcttcatc tccgccatct catcatcaag ctggctgtta tcaatcagct
     1201 cccacatccc ctgtgccaga tgtgtcttcc cgatttctcc tctgaaaaag tccagatacc
     1261 cttccgcatc attgctggcc cggccactgg cttccacaaa cgcagatttc cccaccaggt
     1321 tgacgctgcg cacataaaac cagaaatcct tccccggctt aatgtgcggg ccggagacac
     1381 tccactggcg gcctgtcccc agataacggg cagaggtttc cacctgagat gtgtctgcga
     1441 tttttgcctc cgaaaaccag aactcaaatt gtaccgtcgg gtcatacacc gtaagatgcg
     1501 ggaccgccgt tatctgataa taccccggcg tcagttcaat gctggccggt gctgccggtg
     1561 cgttaatccg gaacgtggtg gtggcaggtt cgccctgctg gccgtagctg tttatcgccc
     1621 tgaccgtcag ggtgtattcc cccagaggca ggccactgaa acggtgctcc gtgtcggcag
     1681 tgatggcggt ggtcaccagg cggctgtttt caccgtttcc gctggtcagg cgcagactga
     1741 agcgcacgcc cttcaccacc cgcggcgtgt cccatttcgc cagtgccaga tactggccgt
     1801 ctgaggcact cacctccacc gtaaggtgct gcaccgccgg cgggatgacg ctgttcagtg
     1861 agccggacat cggctcaaag cgggccccgt tatccacaat ggcttctttt tccggcacat
     1921 gctgcaccgc cgtgatggca aaggtaccgt ccgtgttttc ccggatggaa acacagcgga
     1981 acaggcgacg acgcagtgac ggcagggaga gtccccacac accgtatgtc tccacgccat
     2041 ccggcagggc gctgacctgt atccggtcag gggcggggtg tgcagtgatg tccacgcgca
     2101 tcggcttacc gctgccgtta atcaggttca ccatcgatgt ccctgcttcc agcagtatca
     2161 cctcacggtc cagcgtcagg gtacggctgg cggcatcgat ggacaggatg cgtccgccgg
     2221 tcagggtccc ggcatagtcg ttatcacaga tttcaatgat gtcaccgggt gtgtgacgca
     2281 gcccctgtga cccgagcgtg aaatccaccg tctgcgtttc cagcagttcg gtctttatca
     2341 cccacagtcc ggcacggtgg gcctgaccgc ggctggtaca gccgaacgca tccatcttca
     2401 gcaggttgcg cccgtagcgc aggatgccgt ccgggtcttc caccagttcc gtggaagtct
     2461 gccagccgtt ctgcgggtcg gtgtaattca cctccaccgc cgtgtgccgg tccttcaggg
     2521 cactgaagct gtagcggaac cccacgccgt tatcatccac caccacatcg ctgttggtgt
     2581 agggccacac cacatccgac gggcggtcct gaacgaacgt cagcgtctgg ccgttccata
     2641 ccggcataca gcgcatcgcc gagcagaaat cccccaggac atcccagacc ttacgctgct
     2701 gtgacaggta cgcattaaaa gtcatccgcg gctctgtgcc cccgaaacca tccgggaccg
     2761 tctggtcaca gtactgcccg atggcataca gtgcccactt gtccacatcc gcggccccca
     2821 ggcgttttcc catgccgtag cgcgggtggg tcagcatgtc ccacaggcac caggccgggt
     2881 tattgctgta tgccggtttc agactgccgt cccagatacc gctgtaggtg cgtttttccg
     2941 ggtcatagtt tgacggcacc tgaatgatgc gaccacggat atggtagttc accgtcagct
     3001 gctggccacc aaactgctcc gcatccacct gcagccccac aat
//
LOCUS       DAZGHF010000271         2948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGHF010000271 DAZGHF010000000
VERSION     DAZGHF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.273"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..202
                     /locus_tag="ACHK3A_005009"
     CDS             <1..202
                     /locus_tag="ACHK3A_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO8742946.1"
                     /translation="RYHFELANRGVTRDEGLRNSLRGAIMGLYNCALGSDDREALEEL
                     KVIVAELFEGEIVEPYFTEVAA"
     gene            199..324
                     /locus_tag="ACHK3A_005010"
     CDS             199..324
                     /locus_tag="ACHK3A_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742947.1"
                     /translation="MSIFLSWVILIISVLMAIGIMRIIEAMKNIESLFTGEERRK"
     gene            404..892
                     /locus_tag="ACHK3A_005011"
     CDS             404..892
                     /locus_tag="ACHK3A_005011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742948.1"
                     /translation="MSTNTPTEINKLFTDPAHIELTKKTLTEITNALDERISEIDKDK
                     HFATYMALQMQSMAMVTAKQTISGLYMKNLRLERELAELRAQMKTDESPTEEINARPE
                     HTNAHITMITRKAERTHICELLRELIETAKETHTCPQETCAAIERLIAMLTNPDELKG
                     RA"
     gene            892..1473
                     /locus_tag="ACHK3A_005012"
     CDS             892..1473
                     /locus_tag="ACHK3A_005012"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO8742949.1"
                     /translation="MNHLMIDTETLGNGPDAAIFAIGAVFFDPFTGKLGKQFERFIDP
                     VDSERNGGTVNAATAVWWAGQSVDARACLRNADGTELAAVTEFLAFISRNMHDESPGK
                     TLTVWCKGASFDFPILKSAITRTTGDKSIPWCYWNERCMRPLIDMAESTGWRMPGRQG
                     KETIAHTALNDAIYQAKVVSEIWQRFTAPFLSV"
     gene            1511..2485
                     /locus_tag="ACHK3A_005013"
     CDS             1511..2485
                     /locus_tag="ACHK3A_005013"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFO8742950.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKAHLPEADCLVEPFVGGASV
                     FMNTSYSRYILGDSNGALINFWRTARDNTDSLIDEAKDLFREHNNRTDYLAIRNTFNA
                     ENHAFLSTGISDRRELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGGYRTPYFPERE
                     IRAFADKANSTRTLLVHGDFRKTLSCASHLFSMGNTLCVYCDPPYLPFNARDNFTAYG
                     KPFTKDEHVRLRAALDRLSQETGGMTSITISNSDTPETRRIYQGYRMNCIQAPRSVGA
                     RTREPAPEVIATLKQCDACGRHGGGYCPDCGPVTGYETYSAIFATANT"
     gene            2521..2757
                     /locus_tag="ACHK3A_005014"
     CDS             2521..2757
                     /locus_tag="ACHK3A_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742951.1"
                     /translation="MNSERNKTLLQGLRHIAHAHEETAKLATAVGKPTVVISSAISQG
                     ITIEAIQVIQDQEREIAALKKLLHVKSAGQGVKQ"
     gene            2754..>2948
                     /locus_tag="ACHK3A_005015"
     CDS             2754..>2948
                     /locus_tag="ACHK3A_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742952.1"
                     /translation="MIKESTVIRFTTHGHQYEVDESLIDQGMTRRDSRNPDIYHIRLI
                     NGSHFRATNVEEVRVLTTSCR"
BASE COUNT          872 a          782 c          726 g          568 t
ORIGIN      
        1 gcgttatcac ttcgaactcg caaaccgggg tgtaacccgg gacgaaggac ttcgtaactc
       61 actgcggggc gcaattatgg ggctgtataa ctgcgcactg ggcagcgatg accgcgaagc
      121 actggaagaa ttaaaagtta ttgtggccga actgtttgaa ggtgaaatcg ttgagcctta
      181 tttcacagag gtggcagcat gagcatattt ctttcatggg tgattctgat tatttccgtg
      241 cttatggcga ttggcattat gcgaattatc gaagcgatga aaaatatcga atctttattt
      301 actggcgaag aacgccgtaa ataaataacc aaccggacag aaaacattaa aaccatccgc
      361 atctgcggag ggcttcactc aatccagaaa cggagaaaca gctatgagca ccaatacacc
      421 tacagaaata aacaaactat ttaccgaccc agcgcatatt gaattgacga aaaaaacgct
      481 gactgaaata accaatgcac tggatgaaag aatatcagaa atagataaag acaagcattt
      541 tgccacctac atggccctgc aaatgcaaag catggcaatg gtgacagcaa aacagaccat
      601 cagtggactc tatatgaaaa acctgcgcct cgagcgcgaa ctggcagaac tccgggcaca
      661 aatgaaaaca gacgaatccc ccaccgaaga aataaacgcc agaccggaac acacgaacgc
      721 gcatatcacc atgatcacaa gaaaagccga gcgtactcat atttgcgaat tactgcgcga
      781 gctgattgag acagcgaaag agacacacac ctgcccacag gaaacctgtg cagccattga
      841 acgactgatt gcaatgctca ccaacccgga cgaactgaag ggacgcgcat aatgaaccat
      901 ctcatgattg acaccgaaac actgggtaac ggaccggacg cagccatatt cgcgatcggg
      961 gcagtgtttt tcgacccgtt caccggaaag ctgggaaaac agtttgagcg attcattgac
     1021 ccggtggatt cagagcgcaa cggtggaacc gtcaatgcag caaccgctgt atggtgggcc
     1081 ggacaatccg tggacgccag agcctgcctg cgcaacgcag atggaacaga gctggcagca
     1141 gtgaccgaat tcctggcgtt catttcacgc aatatgcatg acgaaagccc gggcaagaca
     1201 ctgaccgtat ggtgcaaggg cgcttcgttt gacttcccga tcctgaaatc cgctatcacg
     1261 cgaacaaccg gagataaaag catcccctgg tgttactgga acgagcgctg tatgcgtccg
     1321 cttatcgaca tggctgaatc aacaggctgg agaatgccag gccgacaagg aaaagagacc
     1381 attgctcata ccgcactgaa cgacgccatt taccaggcga aggttgtttc agaaatctgg
     1441 cagcgcttca ctgcgccatt cctgagcgta tgaagcggac acacgaaacc tgtaacaaag
     1501 aggccgcgtt atgagcaaag aactgttatc aggcacacca ctgaaatggg tgggcggaaa
     1561 aagccgcatc atgaacacac tgaaggcgca cctgccagaa gccgactgtc tggttgagcc
     1621 gttcgtgggc ggcgcatcgg tattcatgaa caccagttac agccgctaca tccttggaga
     1681 ttccaatggt gcgctgatta atttctggcg gacagcacgg gacaacaccg acagtctgat
     1741 cgacgaggca aaagacctgt tccgggaaca caacaacagg actgactatc tcgccatccg
     1801 aaacaccttt aatgcagaga atcatgcatt cctgagcacg ggaataagcg accgtcggga
     1861 actgcgactg gccgccatgt ttttgtatct caaccggcac tgctttaatg gcctgtaccg
     1921 ggtgaacgga aaaggtgact ttaacgttcc tttcggtggc taccgaactc cctacttccc
     1981 tgaacgggaa atccgcgcat ttgcagacaa ggcaaacagc acccgaacac tgcttgttca
     2041 tggcgatttt cgaaaaacac tgagctgtgc cagccacctg ttcagcatgg gtaacacgct
     2101 ttgcgtgtac tgcgatcccc cttacctgcc tttcaatgcc cgggacaatt tcaccgccta
     2161 cggaaaaccc ttcacgaagg acgaacacgt tcgcctgaga gcagcgcttg accgtctttc
     2221 acaggaaacg ggcggcatga ccagcatcac catatccaat tccgacacac cggaaaccag
     2281 acgtatttat cagggttacc ggatgaactg catacaggca ccacgaagcg ttggcgcaag
     2341 aacccgcgaa ccagcaccgg aggttatcgc caccctgaaa cagtgcgacg catgcgggcg
     2401 ccatggcggc ggctactgcc cggactgcgg gccggtgacg ggatatgaaa cgtacagcgc
     2461 aatatttgcc actgccaata cgtgaataaa cagatgaatc cttaacaccg gagtcaacca
     2521 atgaatagcg aaagaaacaa aactctttta caggggttac ggcacatcgc ccatgcacac
     2581 gaggaaacag ccaaacttgc aactgccgtt ggaaaaccaa cggtcgttat ctcttcagcg
     2641 atttcacagg ggatcaccat agaagcaatt caggtaattc aggatcagga aagagaaatc
     2701 gccgccctta aaaagctact ccacgttaaa tctgccgggc aaggagtcaa gcaatgatca
     2761 aggagtcaac cgttatcaga ttcacaaccc acggccatca gtacgaagtc gacgaaagcc
     2821 tgattgatca gggcatgaca cgccgggatt cacgcaatcc cgatatatat cacatccgcc
     2881 ttatcaacgg ttcgcacttc cgcgccacga acgtggaaga agtacgcgtg cttaccacaa
     2941 gctgccgt
//
LOCUS       DAZGHF010000272         2883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGHF010000272 DAZGHF010000000
VERSION     DAZGHF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.274"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            72..704
                     /gene="comR"
                     /locus_tag="ACHK3A_005016"
     CDS             72..704
                     /gene="comR"
                     /locus_tag="ACHK3A_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO8742953.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(766..1305)
                     /gene="ycfJ"
                     /locus_tag="ACHK3A_005017"
     CDS             complement(766..1305)
                     /gene="ycfJ"
                     /locus_tag="ACHK3A_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO8742954.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1532..2836)
                     /gene="ndh"
                     /locus_tag="ACHK3A_005018"
     CDS             complement(1532..2836)
                     /gene="ndh"
                     /locus_tag="ACHK3A_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO8742955.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          698 a          787 c          702 g          696 t
ORIGIN      
        1 gtggactggt gaacggaggt gattattttt ataataacct ttcattcaca gtcaccaggt
       61 acaacggcat catggcaact gactcaacac aatgtgtaaa aaaaagccgc ggccgcccaa
      121 aagtgttcga cagggatgcc gcgcttgata aggccatgaa attgttctgg caacacggtt
      181 atgaagcgac ttctcttgcg gacctcgtcg aagcgaccgg agccaaagcg cccacgctat
      241 acgcggaatt taccaacaaa gaggggttat tccgcgccgt actcgaccgc tatatcgatc
      301 gttttgccgc taagcatgaa gcacagctgt tttgtgaaga gaaaagcgtg gagtctgcgc
      361 tggctgacta ttttgctgcc atcgccaact gctttaccag caaagacacc ccggcaggct
      421 gcttcatgat caacaactgc accaccctct ccccagattc aggagatatc gccaatacgc
      481 tgaaatcacg ccatgcgatg caagagcgca ctttgcagca gtttttatgt caacgacaag
      541 cgcgcgggga aatcccgacc cactgtgacg tgacgcatct ggcagaattc cttaattgta
      601 ttattcaggg gatgtcgatc agcgcacgcg aaggtgcatc gctggaaaaa ctgatgcaga
      661 ttgcccgaac gactttgcgt ttatggcccg aactggtgaa ataaggcgca aaaaaagccc
      721 ctcagcctga gcgaatgagg ggccaaattg cagagtacag ccttgttata ctttgttatt
      781 caaaatcagt tgcccgttgc tatctagcgg gatctgcgta cccggatcgc ggtccatgcg
      841 gattttgccc tgctgatcgc caatcttata ggtcacatca taaccgagca ttttttctga
      901 cttgtcatac accgttttac aacgctgttg cgtagtcgtg taagtatcgc tttcctggag
      961 agagccctgg atctggttac cggcatatcc accacccagc gcccccacaa cagtagcgac
     1021 atctttaccg cgaccaccac caaactgatg ccctatcacg ccgccagcaa cagcgccgag
     1081 caccgacccg gtaatgcgat tttcatcctg caccggtcga cgatgggtca ctgtgacgtt
     1141 acgacactcc tgacgcggtg ttttaaccgt ttccttgatt ggggttgcag aaacaacctg
     1201 agcgtattgc gggccacgtt caaacacgtt cagactggcc actgccgcta cgcccaacgc
     1261 agctgcgaca ccaatcccga tacccgccaa cattgattta ttcacgggaa tacctccttc
     1321 tttgcagtta tgccaatcat gcaagcgaag gagaaaagca gctatcagac agaagatgaa
     1381 aagcgcggga atggctggaa atacttaggg attcggagga gtctgaagga atgcctgatg
     1441 cgacacttaa cgcgtcttat caggcctacc gctcgtacca tttgtccagg gcggataagg
     1501 cgtttatgcc ccatccgcca gtacaagccg attaatgcaa cttcaaacgt ggacggataa
     1561 cgcggttaat actccccacc agcatcatta atccagtttt aaagtaacca tgcagcgcaa
     1621 tctgatgcat tcggtatagc gagatatata caaagcgcgc aattcgtcct tcaatcatca
     1681 ttgagccgcg tgtcaggtta cccatcaggc taccgacggt ggaaaagttc gacagcgata
     1741 ccagcgaacc gtgatcttta tactgataat ttttcagcgg cttaccgttc atctgcgcca
     1801 gaatgttgtt cattgcgcag gttgccatct gatgtgcggc ctgagcgcgc ggcggaacaa
     1861 agcccccttc cggacgcggg catgacgcgc agtcgccaat agcgtatatg tctggatcgc
     1921 gagtggtttg cagcgtcggt tccaccacca gctggttgat acggttggtt tcaagaccac
     1981 cgatatcttt caggaagtct ggcgctttga tcccggctgc ccacaccatc agatcagcct
     2041 caatatattc gccatcttta gtgtgcaggc cgccttcatc agcactggtg accatggttt
     2101 gcgtcagcac gcgaacgcca agtttcgtta actcactatg ggccgcagca gagatacgtg
     2161 gcggcaatgc aggcaaaata cgttcgcccg cttctaccag cgttacgttc agggcttcgt
     2221 tggtcagacc tttataaccg taactgtgca gttgcttgac cgcgttgtgc aattcagcgg
     2281 agagttctac acccgtcgcg ccgccgccga caatcgcaat gtttactttg ccattcgcgc
     2341 ccaggttggc ggagtatttc aggaacagat tcagcatctc ctggtggaag cgacgcgcct
     2401 ggtgcgggtt atcgaggaaa atgcagttct ctttgacacc tggcgtattg aaatcgttgg
     2461 aggtgctacc cagcgccatt accagggtgt cataggcgat tttacgttcc ggaaccagca
     2521 attcaccttt ttcgtcgcgc agttctgcaa tagtgattgt tttcgcttca cgatcaatat
     2581 caattacgga acccagctgg aactggaaac catgattgcg cgcatgggcc agatagctca
     2641 acgcatcgac gccttcatca agcgagccag tcgccacttc gtgcagcagc ggtttccaca
     2701 ggtggctgtg gttacgatcg accagcgtaa ttttggcttt tttcttgcgt cccagcttat
     2761 gccccagctg tgttgccatt tccagcccac cagcaccgcc gccgacaatc acaatttttt
     2821 tcaatggcgt agtcaacgtg acccccttaa atttattaac caattgttaa ttaatggtta
     2881 tta
//
LOCUS       DAZGHF010000273         2873 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGHF010000273 DAZGHF010000000
VERSION     DAZGHF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.275"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            275..1000
                     /locus_tag="ACHK3A_005019"
     CDS             275..1000
                     /locus_tag="ACHK3A_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8742956.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            958..1635
                     /gene="bglJ"
                     /locus_tag="ACHK3A_005020"
     CDS             958..1635
                     /gene="bglJ"
                     /locus_tag="ACHK3A_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO8742957.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1672..2460)
                     /gene="fhuF"
                     /locus_tag="ACHK3A_005021"
     CDS             complement(1672..2460)
                     /gene="fhuF"
                     /locus_tag="ACHK3A_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO8742958.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNTLSSQLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2601..2837
                     /locus_tag="ACHK3A_005022"
     CDS             2601..2837
                     /locus_tag="ACHK3A_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO8742959.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIIILMGLVLTP
                     AMLYHKQLRHYVLLPSCLALIAGIMLMIMNLKQG"
BASE COUNT          771 a          610 c          708 g          784 t
ORIGIN      
        1 aaattaaatg aatgagaatt ctgtttttca ttaagtattt ctaacaactt aatgtagata
       61 ttttaaatgt ctccaggcta tttcaccagg ttatcatttg atgctaacct tctgacacat
      121 gcagtggagt tgttgtgcag caggagtatg ctgatatgaa agtgaatgct aaggataatt
      181 tattcgctta atctattaat ttgctggaaa catttaagga atgaatatcc tgaataaacc
      241 atcatgattt ttagacagtg gatgtggagg aaatatgttg ccaggatgct gcaaaaatgg
      301 aattgttatc agtaaaatac ctgttatgca agcagggtta aaagaggtca tgaggactca
      361 cttccctgaa tatgaaataa tatccagcgc ctctgcggag gaccttacct tattacaatt
      421 acgtcgttcc ggattagtca ttgctgattt agccggtgaa agtgaagatc cacgttctgt
      481 ttgtgaacat tattattctt taatctcaca atatcgggaa attcactggg ttttcatggt
      541 gtcacgctcc tggtattccc aggcagtaga actgcttatg tgccctacgg cgacgttatt
      601 gtctgatgtt gaacccattg agaatctggt caagaccgta cgttccggca atacgcacgc
      661 agagcgtatc agcgccatgc tgacctctcc ggcaatgact gaaactcatg attttagcta
      721 tcgctccgtc attctcactc tttcagagcg caaggtactg cggctattag gtaaaggatg
      781 gggcatcaac cagatagctt cattgcttaa gaaaagtaat aaaactatca gcgcccaaaa
      841 aaacagtgcg atgcgtcgac tggcaattca cagcaacgct gaaatgtatg catggataaa
      901 tagcgcgcag ggtgcaagag aacttaactt gccttctgtt tatggagatg ccgcagaatg
      961 gaacacagcc gaattaagaa gagaaatgtc gcactcatag aaaaatgcgt catgagcagt
     1021 atcggtattg agagtttatt cagaaagttt gcgggtaacc cttataagct ccatacctat
     1081 accagtcagg agtcatttca ggatgccatg tcgcggatct cgtttgcggc ggtcattttt
     1141 tctttttctg ccatgagaag tgagcgcaga gagggattat cttgcctgac tgaactggcg
     1201 attaagtttc cgcgtacccg gcgtttagtt attgcggatg atgatattga agctcggctg
     1261 attggttcat tgtcgccatc accgttggac ggtgtattaa gtaaagcgtc aacgctggag
     1321 atttttcatc aggaactctt tttgtcatta aatggtgtac gtcaggcgac cgaccgactg
     1381 aacaatcagt ggtacattaa tcaaagccga acgctaagcc cgacggagag agaaatattg
     1441 cgctttatgt cgcgtggcta ctcaatgaca caaattgccg agcagcttaa acgcaatatc
     1501 aaaacgatcc gtgcacataa atttaatgtg atgtcgaaac tgggcgtcag ttctgatgca
     1561 gggttgttgg aggccgcaga tattctgtta tgtatgcggc attgcgaagc aagtaatgtg
     1621 ttgcatccct attaatccac atgatgccgg gtttacttcc ccggcagtgc ttcatttcag
     1681 cgtacaatcg ccacattgct gcacatccgg taagcgataa cgctggcagc aagtgcggcg
     1741 caccagcagg ccgtcgcgca gtaccacggt acgccagagt ggattatctt caccgttcgt
     1801 gagcattttc tcaaaaaaga gggcatggcg cagcgattca accgtcgcct cgccaagcag
     1861 ctgtttcatc tcagtcagat accagttgat caaataaccg gtattgctcc agataagttt
     1921 gccgttgatc tctcccgtcg cttctaatgc ctgcactacc ggaacaagcg cctggctgat
     1981 taacgtttcc attcgctgct gcggcgaatg tagtgttgcg tttttatctt cacacacatc
     2041 gacccagaaa caggcggcgc gtccggtttc gtgaaactca acgtggaaat gttccggcga
     2101 cacatctaat gctttttcct gcgtcagcag cgccaacatt aatggtggca ccatcaggcc
     2161 gatataccat tgtgcccata gtgagatcag cggtttgttc tcgcggatca tcaagggttg
     2221 gttgcggtag atatgatcgg aatagaccgc cagttgagaa cttagcgtat tcggtgatga
     2281 ccattgcgcc agcgtcatgg cacggagcgg tgcgggttca tccagacgga taaactccag
     2341 caaatgttca cgatgttcgg cgatcgtcgc ccgtacggct tgtgcaagcc cagcatcctg
     2401 cggctggaga tgcgttcgcc agataacatc ttcatagagc ggtgcggaac gataggccat
     2461 aatcgtgata gtaatctaaa tgataatgat tgctaatcct agcgataggt ttacccgata
     2521 gcaagggatt tctctggctt gcaaatgata aaaattatca tatgatattg gttatcatta
     2581 tcaatgaaag agatgaaacc atgttgcaac gtacgctggg cagtggctgg ggagtgttgc
     2641 tgccgggatt gctgattgca gggctgatgt atgcggattt atcgccggat caatggcgga
     2701 ttatcattct gatgggatta gtattgacgc cggcgatgct gtatcacaaa cagttgcggc
     2761 attacgtttt gctgccatcg tgcctggcgc ttattgctgg catcatgctg atgataatga
     2821 atttgaagca gggatgataa aacagggaag aaacaagaaa ggaagtaaag ata
//
LOCUS       DAZGHF010000274         2846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGHF010000274 DAZGHF010000000
VERSION     DAZGHF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.276"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..196)
                     /locus_tag="ACHK3A_005023"
     CDS             complement(<1..196)
                     /locus_tag="ACHK3A_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO8742960.1"
                     /translation="MSELSYLEKLLDGAEVEWKTLGKVLKRTKGTKITAGQMKALHKD
                     NAPLKIFAGGKTVAFVDFKDI"
     gene            complement(193..1749)
                     /locus_tag="ACHK3A_005024"
     CDS             complement(193..1749)
                     /locus_tag="ACHK3A_005024"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO8742961.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSHMENGDDSICYAKLDDNVITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLNLGDFNKHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWTGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSARGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTRVQFIDASELFKKEANNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDTREIIDIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQK"
     gene            2013..>2846
                     /locus_tag="ACHK3A_005025"
     CDS             2013..>2846
                     /locus_tag="ACHK3A_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO8742962.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRNT
                     VV"
BASE COUNT          666 a          716 c          696 g          768 t
ORIGIN      
        1 gtatatcttt aaagtcaaca aaggctactg tttttcctcc tgcaaaaatt ttaagcggcg
       61 cattatcttt gtgaagagct ttcatctgcc cagcggttat ttttgtgcct ttagttcgtt
      121 taagcacttt ccctagtgtt ttccactcaa cctcagcccc atccagcaat ttttccagat
      181 aactcaactc gctcatttct gcacctcgca gccttcaatt tcagccacaa tcgcatcaat
      241 atctttacgc aactggtcga ttttgctgac cgtggttttc agctcagcat tcagctcagc
      301 aatatcgata atttcacgag tatctttcgc ttccacatag ctgctcaccg acaggttata
      361 gtcattagcg acaacggtct caaacgcgac tgatttcgcc agatgggcaa catcttcctt
      421 gctggcaaat acctgcataa tctgttcgat atgagcatcg gtcagaatgt tgttgttagc
      481 ctcttttttg aacagttcgc tggcatcaat aaactgaact ctggtatccg ttttatgttt
      541 agacagcacc agtatgttta cggcaatggt ggtgccaaag aacaggttcg gtgccagtga
      601 aatcacggtt tcgacatagt tattgtcgac cagatactga cggattttct gctctgcgcc
      661 gccacggtaa aaaatacccg ggaagcagac aatcgcagcg cgacctctgg ccgaaagata
      721 gttcagcgca tgtaatacaa acgcaaagtc agctttggat ttgggggcca gaacgccagc
      781 cggggcaaaa cgctcatcgt taatcagtgt cgggtcatcg ctgccagtcc atttcaccga
      841 atacggcggg ttagaaacga tggcatcaaa cggtttttca tctctgaagt gcggctcagt
      901 cagcgtattg cccagcttga tatcaaactt gtcgtagttg atgttgtgca aaaacatgtt
      961 catacgcgcc aggttatagg tcgtatggtt gatttcctga ccaaaaaagc cttcttcgat
     1021 gatatggtca tcaaactgct ttttcgcctg caacaacagc gaaccggaac ccgctgccgg
     1081 gtcgtagatt ttgttaacgt gggtctgccc gtgcatagcc agttgtgcaa tcagcttaga
     1141 gacgtgctgt ggtgtaaaga actcgccgcc tgacttaccg gcatttgcgg catagttaga
     1201 aataaggaat tcataggcat cgccaaacag gtcaatctga tgtttgttga agtcaccaag
     1261 gtttaaccct tcaacccctt tcagaacagc agccaggcgg gcatttttat ccttaacggt
     1321 gttacccagg cggttactgg tggtatcgaa atcagcaaac aaacccttga tgtcagcttc
     1381 tgaaggataa ccgtaagcag aactttcgat agcaacgaag atgctgttta aatctgcatt
     1441 cagtctgtca ttggtatttg ctttcgcagc tacgttgcag aaaagctgac tggggtagat
     1501 gaagtagcct ttagtcctga tggcatcgtc tttaatgtca tcagtaatta cgttgtcatc
     1561 cagttttgca taacagatgc tgtcatcacc attttccata tggctggaaa aattttcgct
     1621 gataaaacgg tagaaaagtg cgcccagaac gtattgctta aaatcccatc catcaacaga
     1681 acctctgaca tcattggcaa tttgccatat ctggcggtgc agttctgctc gttgttgaat
     1741 acttgtcatt tttaatccat tttcctggtc tggacctgaa tcattggggg caattctaca
     1801 gcaatcttgt ggttcaggca gccccggagt aagtcggtgt acattgtgag atacctgggg
     1861 tattcacgac aaggaggagg aacggaatac tgcctttcct ttgcagtgca ccaggtacat
     1921 tctaaataat ttcagacaga tattatcgtt gtgagtcccc cgcctccttt ccggtgtgcg
     1981 gggttttgtc ttttttcacc gggaatacat gtatgaatcc gtctgatgcc attgaggcaa
     2041 ttgaaaaacc gctctcctcc ctgccttact cactttcccg tcacatcctg gaacatctgc
     2101 gcaaactcac ccgtcacgaa cccgtgattg gcattatggg taaaagcggg gccggtaaat
     2161 cctcactctg taatgcactg tttcaggggg aggtcacccc ggtcagtgat gttcacgccg
     2221 gcacccggga agtacggcgc ttccgtctga gtggccacgg tcacagcatg gttatcactg
     2281 acctgcccgg ggtgggcgag agccgggaca gggatgcaga gtatgaggcc ctgtaccgtg
     2341 acattctgcc tgaacttgac ctggtgctgt ggctgattaa agccgatgac cgggccctgt
     2401 ctgtggatga gtatttctgg cgacacatcc tgcaccgggg acatcagcag gtgctgtttg
     2461 tggtgacgca ggccgacaaa acggagccct gccatgaatg ggatatggcc ggtattcagc
     2521 cttctcctgc acaggcacag aatattcgcg aaaaaacgga ggcggtattc cgtctgttcc
     2581 ggcccgtaca tccggttgtg gccgtatcgg cccgcaccgg ctgggaactg gatacgctgg
     2641 tcagtgcgct catgacagcg cttcccgacc atgccgccag tcccctgatg acccgactgc
     2701 aggacgagct gcgcacggaa tctgtccggt ctcaggcccg tgaacagttt accggtgcgg
     2761 tggaccggat atttgacaca gcggagagcg tctgtgttgc ctctgttgtc cgtacggccc
     2821 tgcgcgctgt tcgtaacacg gtggtc
//
LOCUS       DAZGHF010000275         2815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGHF010000275 DAZGHF010000000
VERSION     DAZGHF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.277"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(238..732)
                     /locus_tag="ACHK3A_005026"
     CDS             complement(238..732)
                     /locus_tag="ACHK3A_005026"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8742963.1"
                     /translation="MSEFYSRAATVADMPFIMGEFEDGARKGHFYEEILTSKVGDTFE
                     QQLRLEIKANEQGQYTGHFIYILLRRSDDKKVGLIWFCDVRDPTGVPRLELRAVSIVK
                     ELRGKGYGSMLVSDMLASNPSRPMMARCYVKSSQMADMLKRRGFYLFGTSPKGTQMLF
                     RDPK"
     gene            complement(882..1160)
                     /gene="yacB"
                     /locus_tag="ACHK3A_005027"
     CDS             complement(882..1160)
                     /gene="yacB"
                     /locus_tag="ACHK3A_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFO8742964.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(1160..1429)
                     /gene="yacA"
                     /locus_tag="ACHK3A_005028"
     CDS             complement(1160..1429)
                     /gene="yacA"
                     /locus_tag="ACHK3A_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO8742965.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEVGRQQLERGDVLSHDMVESSATAWRDEMSRKVAGK"
     gene            2160..2387
                     /locus_tag="ACHK3A_005029"
     CDS             2160..2387
                     /locus_tag="ACHK3A_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFO8742966.1"
                     /translation="MNSTTHYENANFLRELAESLPRILPEGSTDKSALLQRLANEELA
                     RAEYGEQVRGVVRRKRRNFREAQILCSATCT"
     gene            2583..2693
                     /locus_tag="ACHK3A_005030"
                     /pseudo
     CDS             2583..2693
                     /locus_tag="ACHK3A_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089474.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
     gene            2683..2815
                     /locus_tag="ACHK3A_005031"
                     /pseudo
     CDS             2683..2815
                     /locus_tag="ACHK3A_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          732 a          717 c          652 g          714 t
ORIGIN      
        1 atggtttagg ggtttgaggg ccaaccgtac gaaaacgtac ggtaagagga aaattatcat
       61 cttaaaaatc gattagtaga caatgccgtc cgttaagtgc caattttcga ttaaaaagac
      121 accgttttaa tggcgttttt ctatgtccat tatgtttgat atatcagaca gttacttcac
      181 taacgtacgt tttcgttcca ttggacttca gaccccgcca gcatgggcca gtatctttta
      241 ctttgggtcg cgaaacagca tctgcgtgcc tttgggtgac gtgccaaaaa ggtaaaaacc
      301 tctgcgtttt agcatgtcgg ccatttgcga ggatttgacg tagcacctcg ccatcattgg
      361 ccggctaggg tttgatgcga gcatatcgga cacaagcatt gacccatagc ctttcccgcg
      421 aagctctttg acaatactga cggcacggag ttcaagtcgt ggcacaccag ttggatcgcg
      481 tacgtcacag aaccagatta gtcctacttt tttgtcatca gaacgtcgca gcaggatgta
      541 gataaaatgc ccggtgtatt ggccctgctc gttcgccttg atttccagcc tgagctgctg
      601 ctcaaaggtg tcaccaacct ttgatgtgag gatctcttcg tagaagtgac ctttgcgtgc
      661 gccgtcttcg aactcaccca tgataaatgg catgtctgcc acagttgctg cccttgaata
      721 aaattcactc atgtattacc tcagtatctt tctaagacac catattttat gccccggtta
      781 aaactatgag caaaccagac ggccgcgact gttcagaaca tatcgctctt actgggggag
      841 cagtaaacta aaaatgaaaa gtattattcc tgacaggaat tttatggcca cttctgtgcg
      901 gtatgcaaca cgcgcaggat atacactttt ccccactggc tgtcgacctc ataaaccaga
      961 acaaaatgcg ggtgaataac cagttcccgg gtaccttcca cacgaccgtt gcggccttta
     1021 tacggctgcc cggccaggct gctggctgaa taaccaatcc gttcgtccag ttcaatggca
     1081 gccatcaggt tctgctccgc aatgtactca cgaatgcgct tcctgtcctg gctggccagc
     1141 atggtccaga atatctccat catttaccgg caaccttcct gctcatttca tcccgccatg
     1201 cggtagctga ggattcgacc atgtcatggg aaagcacatc accgcgctca agttgctggc
     1261 gtcctacctc aacctggtca cggaaccagg tattatgctg ccgttcaacg gtctgacgca
     1321 taaaatcccg gattaactga gagccattac ggtccatgct ttttgcagcg gccataaaag
     1381 catccttcag ttccgcgtcg attcttaaac tcatattaac ctgtgccatt tgccacctcg
     1441 tcattaccac gcatataccc agtatataac gattcagata tcgttaccag aatatgccgc
     1501 gtcagcggca tggaaggcgg cactctgttg tttcatatga taccggagta aaaccgccga
     1561 agcccggcgt aagccggtac tgattgatag atttcacctt acccatcccc gcccttggcc
     1621 atatctgctt tcagccatga taaatcttct gtgcgcggtc ggagtggtcc cgacgagggt
     1681 ttacccgaag tcggggcgta tctccgcgtt agcgggccgt cagggccgct tacgagcgtg
     1741 ttctgagggc ttccagccag aagaggggtt tgaagcccaa tggagcggga acgtacgcta
     1801 agaagctaac tatatgattt taagtcaata aagtcgtggt gttgttaggc aggtttacgt
     1861 tgatttgatt accgagttta ttaacactga aagtccctga aaccgcatgg ctactggcat
     1921 tgacgggaag agtaaacaaa accctcgttt tctttactca cctcttctgc tcaaaaaatg
     1981 aacttagcgt acgatctcgt acggttgggc ctcaaacccc tctactaatc gatttttaag
     2041 acgataattt tcctcttacc gtacgttttc gtacggttgg ccctcaaacc ccttatactc
     2101 atgcgtgcgg tatcaaaaaa tctgcaatta ccctttttga atgaaggagg gagtcaacta
     2161 tgaatagcac cacacattac gaaaacgcca atttcctgcg cgagctggcc gaaagcctgc
     2221 ctcgtatttt gccagaaggc agtacagaca agtctgcact attgcaacgc ctcgctaatg
     2281 aagaactggc acgggcggaa tacggcgaac aggtacgagg ggttgtacgc cggaaacgcc
     2341 gaaatttccg ggaggcacaa attttatgct cggcaacctg tacgtaagac cgccgttaac
     2401 cggacagcaa agaaatagct gtttagagcg aaagaatcac tgcccgtaac gtatcacata
     2461 gctggtacgg ataaattctc acttactgca ccataaacgt actcgtcaac aaccgccaaa
     2521 catgcttcaa aaatcaacaa attcgctgta tcggaaattt cggcgtttcc ggcgtataac
     2581 ccctacgagc gaaaattgcc gctgctcgag ccgataagcg accgggaata agttccgcgc
     2641 aactgcgcca gcaactacag ggacgttatc aggaactgcg caatgagtta tgaaatagaa
     2701 tggcgaccag gtgcgattga ggatataacg gcgttgtttg aatatctggc cgagaaagaa
     2761 tgcatctctt tgggatgcaa ggaacgtgac agagcgtctg atcactgcaa ccgat
//
LOCUS       DAZGHF010000276         2790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGHF010000276 DAZGHF010000000
VERSION     DAZGHF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.278"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(40..116)
                     /locus_tag="ACHK3A_005032"
     tRNA            complement(40..116)
                     /locus_tag="ACHK3A_005032"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Asp,seq:gtc)
     gene            complement(249..980)
                     /gene="dnaQ"
                     /locus_tag="ACHK3A_005033"
     CDS             complement(249..980)
                     /gene="dnaQ"
                     /locus_tag="ACHK3A_005033"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO8742967.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQDETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1045..1512
                     /gene="rnhA"
                     /locus_tag="ACHK3A_005034"
     CDS             1045..1512
                     /gene="rnhA"
                     /locus_tag="ACHK3A_005034"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO8742968.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1509..2231)
                     /locus_tag="ACHK3A_005035"
     CDS             complement(1509..2231)
                     /locus_tag="ACHK3A_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8742969.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGLPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2265..>2790
                     /gene="gloB"
                     /locus_tag="ACHK3A_005036"
     CDS             2265..>2790
                     /gene="gloB"
                     /locus_tag="ACHK3A_005036"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO8742970.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFA"
BASE COUNT          720 a          693 c          672 g          705 t
ORIGIN      
        1 atttttgcgg ttctctgaac tcattttcat gagtgaattt ggcggaacgg acgggactcg
       61 aacccgcgac cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccgcgt
      121 tttgttccgt tgggaacgag gcgaatatta cggattgcct cacccttcgt caacggtttt
      181 tctcatcttt tgaatcgttt gctgcaaaaa tcgcccaagt cgctattttt agcgcctttt
      241 acaggtattt atgcccgcca gaggcagctt ccccccttct tctgcaccag atcgagacgg
      301 gcttcatgtg ctgcaagctc ttcatctgtc gcaaaaacaa cgcgtaactt acttgcctga
      361 cgcacaatgc gctgaattgt tgtttcatct tgttgctgtt gtgtctctcc ttccatcgca
      421 aaagccatcg acgtttgccc accagtcatc gccagataaa cttccgcaag gatctgggca
      481 tcgagtaatg ccccgtgcag cgttcgttta ctgttatcta tttcgtagcg ggcacataac
      541 gcatcgaggc tgttgcgctt accgggaaac attttcctcg ccaccgcaag gctatcggtg
      601 accttacaga aggtattggt cttcggaata tcgcgcttaa gcaacgaaaa ctcgtagtcc
      661 ataaagccga tatcgaacgc tgcgttatgg atcaccagct ccgcgccgcg gatatagtcc
      721 atgaactcat cggctacctc tgcaaacgtg ggcttatcga gcaaaaattc atcggcaata
      781 ccatgtacgc caaaggcttc cggatccacc agccgatcgg gtttgagata aacatggaag
      841 ttattgcccg tcaggcgacg gttcaccact tcaacggcac caatctcaat gatcttgtgg
      901 ccttcatagt gcgcaccaat ctggttcata ccggtagttt cggtatcgag aacgatctgg
      961 cgtgtaattg cagtgctcat agcggtcatt tatgtcagac ttgtcgtttt acagttcgat
     1021 tcaattacag gaagtctacc agagatgctt aaacaggtag aaattttcac cgatggttcg
     1081 tgtctgggta atcctggacc tgggggttac ggcgctattt tacgctatcg cggacgcgag
     1141 aaaaccttta gcgcaggcta cacccgcacc accaacaacc gtatggagtt gatggccgct
     1201 attgtcgcgc tggaggcgtt aaaagaacat tgcgaagtca ttttgagtac cgacagccag
     1261 tatgtccgcc agggtatcac ccagtggatc cataactgga aaaaacgtgg ctggaaaaca
     1321 gcagacaaaa aaccagtaaa aaatgtcgat ctctggcaac gtcttgatgc tgcgctgggg
     1381 cagcatcaaa tcaaatggga atgggttaaa ggccatgccg gacacccgga aaacgaacgt
     1441 tgcgatgaac tggctcgtgc cgcagcgatg aatcccacac tggaagatac gggctatcaa
     1501 gtcgaagttt aagcctgtgg tttacgacat tgccgggtgg ctccaaccgc ctggcgaatt
     1561 cgtggcttgt ttttactctg tttcatcgga tttagcgtta aaggaatggt ccgtttccgg
     1621 gcaacaataa gttgtaagca accaagcgca ggaatatgcg cattcaatag ttttcctccg
     1681 tgtttgttcc acgggagaac gtggaaacgg ctggcgtgta gcacttcaaa attcaacaaa
     1741 gagagccagt ccagctgccg catcagagta aacatccggc tgttataggg cgaggttttg
     1801 cgcaataccg gcacaagttt gcgtaatccc attaaactga tgggattgaa gccactaatc
     1861 accagccagc catcatcaat caatacccga tcggcttcac gcaataaacg atgcggatcg
     1921 gtgcaccacg gcaatgtatg tgccagtaga caaacatcaa cggatttatc ggcaaaagga
     1981 agatgaagtg ggtccgcctg cacctggacg ggcagtcctt gcgcagaaac attcacttga
     2041 tgagaaaccg cgcacgcttc gcaattgatt tctgcgctta aattgccaat cttaagcaga
     2101 tgaaaaccat acattttagt gaaccacggg ttgagctggc gctccagcgc cttgcgataa
     2161 agctctcccc agggcaaatc gccccagcaa tcaggagcca cgacagtttg agggactctt
     2221 gccggtttca tcacaacctt ccgtttcaca ctgagaggta atctatgaat cttaacagta
     2281 ttcccgcctt tgatgacaat tacatctggg ttttgaatga tgaagcaggt cgctgcctga
     2341 ttgtcgatcc cggagacgca gagccagtat taaacgccat tgccgccaat aactggcaac
     2401 cggaggccat atttctcacc caccatcacc acgatcacgt tggcggcgta aaagaactgg
     2461 tgaaaaagtt tccgcaaatt gtggtgtatg gtccacaaga gacacaagat aagggaacaa
     2521 cacaggtagt caaagatggc gaaactgcct tcgttttggg gcatgaattt agtgtaattg
     2581 ccacgccggg tcacacttta ggacatatct gttacttcag taaaccttat ctattttgcg
     2641 gcgacacgct gttttctggt gggtgtggtc ggttgtttga agggacagca tcacaaatgt
     2701 atcaatcact taaaaagtta agtgcgttac ctgacgatac attggtatgt tgtgctcatg
     2761 aatatacctt atcaaatatg aagtttgctt
//
LOCUS       DAZGHF010000277         2777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGHF010000277 DAZGHF010000000
VERSION     DAZGHF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.279"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            95..2374
                     /gene="ydeP"
                     /locus_tag="ACHK3A_005037"
     CDS             95..2374
                     /gene="ydeP"
                     /locus_tag="ACHK3A_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO8742971.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTIGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2622..>2777
                     /gene="safA"
                     /locus_tag="ACHK3A_005038"
     CDS             2622..>2777
                     /gene="safA"
                     /locus_tag="ACHK3A_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO8742972.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIA"
BASE COUNT          746 a          652 c          673 g          706 t
ORIGIN      
        1 cttatatatc catgttgacg ataatccctt tgtatatact gtgcatcatc gctattacaa
       61 atcctaataa ttcatttcca cacaggataa gcagatgaag aaaaaaattg aatcctacca
      121 gggagctgcc ggtggttggg gtgctgttaa atccgtagca aatgcagtac gtaagcagat
      181 ggatatacgc caggatgtta ttgccatgtt tgatatgaat aagccagagg gctttgactg
      241 tccgggatgt gcatggccag atcctaagca cagtgcgtca ttcgacattt gtgaaaacgg
      301 cgcaaaagca atcgcctggg aagtcacgga taagcaggta aatgcctctt tctttgctga
      361 gaatacggtt caatcattac ttacctgggg agatcacgag cttgaggctg cgggacgtct
      421 cactcagcct ttgaaatatg atgacgtcag cgactgttac aagccattaa gctggcaaca
      481 agctttcgac gaaattggcg aacgccttca aagctatagt gatcccaatc aggttgaatt
      541 ctatacttcg ggccgcactt ccaatgaagc tgcctttctt tatcagcttt ttgcccgtga
      601 atacgggagc aataacttcc ccgactgctc caacatgtgc catgaaccga ccagtgtggg
      661 tttggcagcg agtatcggtg taggtaaagg gaccgtgttg ctggaagact ttgagaaatg
      721 cgatttagtc atttgcattg ggcataaccc tggtacaaac caccctcgca tgttgacttc
      781 gttgcgcgct ttagtgaaac ggggagcgaa aatgatcgcc atcaatcctc tacaggaacg
      841 tggcctggag cgatttaccg caccgcaaaa cccgtttgaa atgctgacga actctgagac
      901 tcagttggcc agtgcctact acaacgtgcg cattggtggt gatatggcgt tgctcaaggg
      961 gatgatgcgc ctgttaattg agcgcgatga tgctgcaagc gccgcaggtc ggccctcatt
     1021 gctggatgac gaatttattc aaacgcatac catcggcttt gacgagctac gccgtgacgt
     1081 gctcaattcc gagtggaaag atatcgaacg tatttctgga ctaagtcaga cacaaatcgc
     1141 cgaacttgct gatgcatatg ccgctgccga acgaaccatt atctgttacg gaatggggat
     1201 cactcagcac gaacatggta cccagaacgt acagcaactg gtcaatctgc tgttgatgaa
     1261 aggtaacatt ggtaagcctg gtgcgggtat ctgcccacta cgaggtcact ctaatgtaca
     1321 gggcgaccga accgtcggta tcaccgagaa accgtctgca gagtttctgg ctcgcctggg
     1381 tgagcgctat ggtttcaccc cacctcatgc acctggacat gctgcaattg ccagcatgca
     1441 agcaatatgt acggggcagg ctcgagcatt gatctgcatg gggggcaact ttgcactggc
     1501 aatgccagat cgggaagcaa gcgctgtacc gttaacacaa ttagatctga cggtacacgt
     1561 agccactaag cttaaccgct ctcatctgct gaccgcacgg catagctata ttctgccggt
     1621 tctgggacgc agcgagattg acatgcaaaa aagcggtgcg caggcggtaa ccgttgagga
     1681 ttcaatgtcg atgattcatg cctcgcgtgg cgtgttaaaa cccgccggtg taatgctgaa
     1741 atcagagtgt gcagtggtcg cgggaatcgc gcaggcagca ctaccccaga gcgtggtagc
     1801 ctgggagtat ctggtggaag attatgatcg cattcgcaat gacattgaag ctgtgctgcc
     1861 agagttcgcc gactataacc agcgcatccg tcatcccggt ggttttcacc tgataaatgc
     1921 agctgctgaa aggcgctgga tgacgccgtc aggtaaggct aatttcatta ccagtaaagg
     1981 gctgttagaa gatccctctt cagcgtttaa cagtaaactg gtcatggcga cagtacgcag
     2041 ccacgatcag tacaacacga cgatttatgg tatggatgat cgctatcgag gtgtattcgg
     2101 tcaacgagat gtggtcttta tgagtgctaa acaagctaaa atttgccgtg taaaaaacgg
     2161 cgaaagagtt aatcttattg cgctcacgcc agacggtaaa cgtagctcac gccgcatgga
     2221 tagattaaaa gtggtcattt accctatggc tgaccgctca ctggtgacct attttccaga
     2281 atcgaatcac atgctaacac ttgataacca cgatccatta agtggcattc ctggctataa
     2341 aagtattccg gttgaattag aaccatcaaa ttaattcgcc tcctcatttc ttctgctgtc
     2401 atccgcacag cagaagaatt cctcattgac cattgtcgcg caatttactc acatgtgcta
     2461 aattaaactg ctcgttataa gatatatacc tccgcctaca gctgtaagaa actccgctca
     2521 gtactgaagc gccagtccta tttcccctat tctccagcct gttatattaa gcatactgat
     2581 taacgatttt taacgttatc cgctaaataa acatatttga aatgcatgcg accacagtga
     2641 aaaacaaaat cacgcaaaga gacaactata aagaaatcat gtctgtaatt gtggttgtct
     2701 tattactgac acttacgttg atagccattt tttcggcaat tgatcagctg ggtatttcag
     2761 aaatgggtcg cattgca
//
LOCUS       DAZGHF010000278         2718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGHF010000278 DAZGHF010000000
VERSION     DAZGHF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.280"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1571
                     /locus_tag="ACHK3A_005039"
     CDS             <1..1571
                     /locus_tag="ACHK3A_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO8742973.1"
                     /translation="KAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAKLIPF
                     LAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGFMAMH
                     LGTNFYLKQHDLLVVADQMSTHGQNIILGIQSYNTSVLGGIVAGLLVASMYKKIVNLR
                     IPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVGYFFY
                     AVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIPLDLA
                     GKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIEFLFL
                     FVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAVCVEV
                     AYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADTPTLDKSELQAAEQMV
                     KALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKRAGGIAVVKLDQNTLQVIIGTKV
                     IALRRDMDNYMGMR"
     gene            1571..>2718
                     /locus_tag="ACHK3A_005040"
     CDS             1571..>2718
                     /locus_tag="ACHK3A_005040"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFO8742974.1"
                     /translation="MDIFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYHQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNAFFDVIKHSERNILTASLIIKNEGGYAINWQDFDLKIK
                     SAKMVLLCNPHNPTGTVWSEEELLKIATSCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYLISTDEDYNHKFRTISRYRDFVNSPSVLNIIATI
                     VAYNECEGWLKSLKAHIASNIQFTQQYLNENTPELIVRPADGCYFSWIDCSAIGYSFD
                     EFYSRLIHEGKVGIMAGNVYGTEGEGYLRLNLACGREKLYMGLTRLASV"
BASE COUNT          736 a          508 c          633 g          841 t
ORIGIN      
        1 agaaagcctg gagttttttt cagagcctgg ggaaggcatt tatgtatccc attgctctgc
       61 taagtgtatg tggcatgatg ctagggctgg gaagtggttt agccagtgat gatatggcaa
      121 agttaattcc atttctggct attccaataa ttaaaaccat acttgatttc attgttagtc
      181 ttggtttgtt tgcctttgtt aatttacctg tattgtttgc gatagcgatt cccttaggat
      241 tattaaaaga taaagaggat aaagcctatg gtgctttttc tggcttaatt ggttttatgg
      301 cgatgcatct gggaacgaac ttttatctta aacagcacga cttattggtc gttgctgacc
      361 aaatgtcgac acatgggcaa aacatcattc tggggatcca gtcctacaat accagcgtgt
      421 tagggggaat tgttgctggg ttattagtcg ccagcatgta taaaaagatc gttaatttac
      481 gcattccaga atcattaggt ttttatagcg gcccacgtct ggtgcctatc attacactga
      541 ttgtgatgag tggatttggt ctgatcattc cttttatctg gccgccattt ttcaatctct
      601 tcatgctcat tggccactgg atatcaactt ccggtcctgt tggttatttc ttctatgcag
      661 ttgccgaacg tgtgacgatt ccttttggct taaaccatct ggtgacgtca gtgttccgct
      721 ttacgccaat cggcggttcg gctgtgattg gcggcgaaga gtattacggc accctgaaca
      781 tgtttatggc gtacgtcaaa gagaatgcgg tcattccgct ggatttggcg gggaaaatgg
      841 agcagggcaa actgatgatt cagtacggtc tggctggtgc tgcactggcc atgtatcgca
      901 ctgctcatgc tcaaaacaga aaggctatca aagcattgct tatttccggt gtgcttacgg
      961 ttattattgg tggcgtcagc gaaccgattg aatttctgtt cttatttgtc agtccgctgc
     1021 tgtttgtctt ccatgccttt atgaatggat tcgccaacat ggtcttgcca tatatgggcg
     1081 tgaagatggg atttactggc gatctgattc aatttattag ctttggcgta ttgcgtggca
     1141 caagaacagg ttggccgatc gcggtgtgtg ttgaagtggc ctatttcttc atttattact
     1201 ttgtgttccg ttggaccatt ctcaaattta acctgatgac cgtaggacgt gaagagtcca
     1261 gtcctgtcac gctgaacgct cacgaagata cggctatagc ggatacccca actcttgata
     1321 aatcagagtt gcaagcagcg gagcagatgg ttaaggcact tggtggtaaa gagaatatta
     1381 agtcactgga taattgcgta actcgtttac gtttaacaat cgcagatatg ggattgattg
     1441 atgaagttgc aataaaaaga gctggcggga ttgcggttgt taaacttgat caaaataccc
     1501 tacaagtcat tatcggcact aaagtcatcg ccctgcgtag ggatatggat aactacatgg
     1561 ggatgcgctg atggatattt ttaatactcc tatttcccgt aaaggaacat attgcaccca
     1621 atgggacttt tgcgaggaca gatttggtgt aaaagatgta ttacccttct ctatttcaga
     1681 tatggatctt ccgataccag acgctatcac cagagcatta aaaaaacgcc tggaacaccc
     1741 gattttaggg tacagtcgtt ggcaacatgg tgaatatctg aatgcgatag taaactggta
     1801 tcatcaacaa tatcaaacag acattaaacc tgaatggata acctatagcc ccagtgtgat
     1861 gtactctatt gcaaaggcaa tagagttatt gacatcgcat ggtgataata ttctcgtttt
     1921 tacacctgtc tataatgcat tttttgatgt aattaaacat agcgagcgaa atattctgac
     1981 agcctctttg attataaaaa atgagggggg ctacgctatc aactggcaag attttgatct
     2041 taagattaaa agcgcaaaga tggtgttatt atgtaatcct cataatccga caggaacagt
     2101 ctggtcggaa gaagaactgc ttaaaatcgc tacaagctgt acaaggcata atgtctggtt
     2161 atgttcagat gaaattcata gtgattttgt ttttaatcgt agctttacat ccgcgcttaa
     2221 aataaaaaaa gaaaaagtag tggtatttaa ctcgatatcg aaaacattta atgttcctgc
     2281 gctaacagga tcgtatctga tttcaactga cgaggattat aatcataagt tcagaacgat
     2341 atcgaggtat cgcgatttcg taaactcccc atcagtactg aatataattg caaccattgt
     2401 tgcctataat gaatgtgagg gatggttaaa gtcattaaag gcacatatcg cctctaacat
     2461 tcaattcacc cagcagtatc tgaacgaaaa tactccagaa ttgatcgtaa ggccagctga
     2521 tggttgctat ttttcgtgga tagattgctc tgctattggt tattcctttg atgaattcta
     2581 cagtcgatta attcatgaag gcaaagtggg cattatggcg gggaatgtct atggaacgga
     2641 gggggagggc taccttcgtc tgaacctggc ctgcggtcga gaaaaattat atatggggct
     2701 tacgcgtctt gcgagtgt
//
LOCUS       DAZGHF010000279         2699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGHF010000279 DAZGHF010000000
VERSION     DAZGHF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.281"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            307..990
                     /locus_tag="ACHK3A_005041"
     CDS             307..990
                     /locus_tag="ACHK3A_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO8742975.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQSA
                     ETRQRKNKK"
     gene            1034..>2699
                     /locus_tag="ACHK3A_005042"
     CDS             1034..>2699
                     /locus_tag="ACHK3A_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFO8742976.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISVLVASG
                     LLSSFGALANNYDGQPTDYGDGSAGDGWVAIGKGAKANTFMNTSGASTALGYDAIAEG
                     EYSSAIGSKTLATGGASMAFGVSARAMGDRSVALGASSVANGDRSMAFGRYAKTNGYT
                     SLAIGDSSLADGEKTIALGNTAKAYEIMSIALGDHANASKEYSMALGASSAASAANAI
                     AVGRNSAAAGVDSLAFGRLSAANAANAIAMGAESKAAENATAVGTNAEANGLNSIALG
                     SGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIALGQG
                     SYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGDIST
                     TSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTGLDN
                     NTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATNTAN
                     ITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTEATSKITNVKD"
BASE COUNT          799 a          593 c          654 g          653 t
ORIGIN      
        1 ctatcagcat cgcaaacttc gcaataaaac atcactcaac tgaatattta cctttccctg
       61 ccctgggcct ttatccggtg aaacatttga cattgattga caccgatcat taaaatccgc
      121 gcgattaata ttattggcta caaaccgaaa ttgtttgaca cttaataaca tattcgcaca
      181 accgattaat acaaaaattc tctaacaaat ttatttgtcg gatatgctcc actctaatca
      241 ttcataaaaa aaatgattgt gccgtaatta tcaactgtat cgggattatg aaaaggaatt
      301 catcctatga acctaaagaa aacactgtta agcgtgttaa taatagtgcc tctctgctta
      361 ttggcagggt gtgactatat tgaaaaagcg agtaaggtcg acgacctcgt tacacagcaa
      421 gagttgcaaa aaagcaaaat tgaggcgctt gagaaacaac aggaactcga taagcgaaag
      481 atagaacatt ttgaaaaaca acaaactact atcataaata gtaccaagac gctcgctggc
      541 gtggtgaagg cggttaaaga caaacaggac gaatttgtct ttacagaatt taacccggca
      601 caaacccaat acttcatttt aaataacggc tctgttggtt tggcaggccg ggtgctggct
      661 attgatgcag ttgaaaatgg cagcgttatt cgtatttcgc tggttaattt attaagcgtt
      721 cccgtatcaa atattggctt tcatgcgaca tggggcaatg aaaggcccac cgatgccaaa
      781 gctctggcaa agtggcaaca attacttttc aacaccacac tgaattccac tctgcaatta
      841 atgcctggtc aatggcaaga cattaatctg acattaaaag gcgtttcgcc taataacctg
      901 aagtatctga agttgtccat caatatggcc aatctccagt tcgacactgt tcaatctgct
      961 gaaactcggc agcgaaaaaa caaaaaataa atgcagcttc tgcgcttata tcaaggaata
     1021 gagagcatca ataatgaaca aaatttttaa agttatctgg aatccagcga cgggcagtta
     1081 cagcgttgcc agcgaaacgg cgaagagccg tggtaaaaaa agcgggcgca gtaagctgtt
     1141 aatttctgta ctagttgcga gtgggttgtt gtcgtcgttt ggagcgttag ctaataacta
     1201 cgacgggcag ccaactgatt atggcgatgg ctcagcaggt gacggctggg ttgctatcgg
     1261 taaaggggca aaagcaaata cctttatgaa cactagtggc gcgagtacag ctttaggata
     1321 tgacgcgata gccgaaggtg agtacagttc tgccatcggg tcaaaaaccc ttgcaactgg
     1381 tggagcatcc atggcgttcg gggttagtgc aagagcaatg ggtgacagaa gtgtcgcgct
     1441 gggtgcatcg tcagtagcaa atggcgatcg ttcgatggct tttggtcgtt acgcaaagac
     1501 gaatggttat acatctcttg ctattgggga ctcctccctt gccgatggtg aaaaaactat
     1561 tgcgttagga aatacggcta aagcttacga aattatgagc atcgccctcg gtgatcatgc
     1621 caatgcgtca aaagaatatt caatggcgct gggtgcaagt agtgccgcat ctgcggctaa
     1681 cgcgattgca gtaggccgca acagtgctgc agctggcgta gacagcctcg cattcggtag
     1741 actatccgcc gccaacgccg caaacgcgat tgctatgggt gcagagagca aagctgctga
     1801 aaatgcgacg gcagttggta ctaacgcgga ggcaaatgga cttaatagta tcgcgttggg
     1861 ctccggcagt attgcagatg tcgacaacac tattgctctg ggcaatcaaa gtcaggcagt
     1921 agcggctggc gcaattgcca tcggtcaagg gaataaagct gacggcgcaa atgctatcgc
     1981 gctgggtaat ggtagtatta caggtggtgt aaatgctatt gctcttggac aaggcagtta
     2041 tgccggttta gaaaatggca ctgcaattgg tgctcaagcc agtgctcagg ggaaaaattc
     2101 agttgctctg ggtgctggtt ctgtagcgac tgacgcggat actgtttctg tgggtaacac
     2161 aacagctcag cgacaaattg tcaatatggc agcaggtgat atcagcacta ccagtactga
     2221 tgccatcaat ggatcacagc tttatgctat cagtaagtca gtagcggaca atcttggtgg
     2281 gggggctacc gtcaatgcgc aaggcgtcgt tacttcccca aattacaggc tgaaaagtgg
     2341 tattttcggc actgttggcg acgccttaac gggcctggac aataatacgt tacaatggga
     2401 ctcccttaaa aaggcatata gtgcggcaca tggtacagat actaccagta ccatcaccaa
     2461 cgttaaagac ggcgctattt ctgataccag taaggatgcg gttaacggtt ctcagctgaa
     2521 aaccaccaac gataacgtag cgaccaatac tgccaatatc accaccaaca ctaacagtat
     2581 caataccctg acggattccg ttggcgacct taaagacgat gccctgctgt ggaatggcac
     2641 cgcgttcagc gccgcgcacg gcaccgaagc caccagcaaa atcaccaacg tcaaagacg
//
LOCUS       DAZGHF010000280         2655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGHF010000280 DAZGHF010000000
VERSION     DAZGHF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.282"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(149..910)
                     /locus_tag="ACHK3A_005043"
     CDS             complement(149..910)
                     /locus_tag="ACHK3A_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO8742977.1"
                     /translation="MKKIIAVSALAVVGMFSGLALADRGKTGFYMTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTSSAFTPWVSVGLGYARI
                     HHKATYTDTSWNKSGEVSNFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLD
                     AGDASVSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            1700..>2655
                     /gene="subA"
                     /locus_tag="ACHK3A_005044"
     CDS             1700..>2655
                     /gene="subA"
                     /locus_tag="ACHK3A_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO8742978.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHE
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRKMSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEG"
BASE COUNT          760 a          606 c          604 g          685 t
ORIGIN      
        1 caacattatt atgaagagaa taaaataata agaaaaacag aaatatcctg taaagcagat
       61 aacagacaac cctgcagtat aattacggca gggagtttcc gtttaattac agaagacagg
      121 taattattat ctttcaggag tagtttcgtc agaagttata agtcacacca agcatgatgt
      181 catgactttt aacatcaact tctgatttat atttatcgcc ccactcatcc ttgtaactca
      241 cactggcatc acctgcatca agatagcgat agctgaggtc cagagcgata tccggggtta
      301 cgtcatagcg gacacctgcc ccaaaactcc atgcaaaatt gttatcatat cccgggtaat
      361 gcaacgcaga aaaattgctc acctcaccag atttattcca ggaggtatca gtatatgttg
      421 ccttatgatg tatccgtgca taacccagtc caacagacac ccatggagtg aatgcactgc
      481 tggtgaatgc actgctgttt ctgaagtcat aatacgtatt caccatcaga gtattcacac
      541 tgagcctgtt ctgtgtgtct tcccgaccac caccgaccat cggagagtac catgtatcca
      601 gtgtataacg ggagtccgca gctccgcggc catagaattc cacttccgtg cgcacaggaa
      661 cattgtaatg ctgataaaaa tcatagccca ccgcaaggcc ggcaccgaat accgtatcgt
      721 tcttatcgcc gcccttatac ttataaacat cgtccccaaa gtcctgacga aagcgctggt
      781 cagtctgagt cacaaccgaa gcaccagctt taccggtcat ataaaagcct gttttgcctc
      841 tatcagccag ggccaggcct gaaaacatgc ccaccacagc aagcgctgaa accgcaataa
      901 tctttttcat ttcagttatc tccgctatca ctttccccga acgataaacg tggttgctta
      961 ttctaaaccc atgcaaaaga cagggtaaaa agagaaaaat cgatcgaatc gatcgacaaa
     1021 atcgatcatg catgatgaag atttcttatc gaatacataa aaatagtgat ggcaatcgtg
     1081 gcaggagctt acgtcaggcc ctgaagtact gacacaggca ttgatggcag gtgatataag
     1141 gagtgctcca gggaatgact cccgactgac ccaggcactg tcccgttgtc aacccgtatg
     1201 gttactgctc tgactcattt cctgtcgtca gacgttcaac tctggaccac tggttatgac
     1261 gacaggtgtc gctcatttgt cagcactata acggtcaaag atcgtggcat tgacacattc
     1321 tccagaggta aatttctcaa gaggtaacaa ctctgccagg aggatatctg cagagagtta
     1381 cattcgtttg ggttcatatc tgtgccgcgc gttttccttc catcagtgaa caaacattca
     1441 gtcccatgac gaccgggcaa atagcgtgtt cacagatatt gctgtccagg tacgaagtac
     1501 cataatggat attggtaagt tattgggtga cccccctcca tgaaaaaacc aaatataaaa
     1561 atgaatgttt cgggagagac ttcactcgct ttattgatag tgattgttta tcttttactg
     1621 aagacaggat atttcagatg tgatatccct gtaacatatt gaccagcaat actttttatt
     1681 tgtaccactg gaggatattg tgctgaaaaa tttacggctg catattctgt tcctgctttt
     1741 tatctcagtg tcagtcaggg cggaaaaacc atggtatttt gatgcaattg gtctgacaga
     1801 aacaaccatg tcgctcacag acaaaaatac cccggttgtc gtatctgttg ttgattccgg
     1861 agtggcattt gtaggaggcc tcagcgacag cgaatttgcc aaattcagtt ttactcagga
     1921 tggttcgcca ttcccggtaa aagagcctga agctttatat attcatggta ctgccatggc
     1981 ttcactcatt gcctcacgtc atgaggttta tggtgtttat cctcatgcac tgatatccag
     2041 cagaagggtt attcctgacg gtgtacagga ctcatggatt agagcaactg aaagcattat
     2101 gtcaaatgtt tttcttgcac caggagaaga gaaaatcatt aatatatcgg gaggccagaa
     2161 ggggatatct tccgcatcgg tctggtccga actgctttcc cgtatgggcc ggaataatga
     2221 gcgattaatt gttgcggcag tgggtaatga tggcgcagat atacgaaaaa tgagtgctca
     2281 gcagagaata tggcctgcgg cttaccatcc ggtcagcagt gtgaataaaa agcaggatcc
     2341 tgtgataaga gtggcagctc tggcacagta ccggaaagga gaaacacctg tattgcatgg
     2401 tggaggggtc accggaagtc ggttcggaaa cggctgggtt gatattgccg caccgggaca
     2461 gaatattaca ttcctcagac cagatggaaa aacggggatc ggtagtggaa cctcggaagc
     2521 aacagctata gtttccggcg ttctggcagc gatggtctca tgtaatcccc gtgcaacagc
     2581 gacagaactg aagcgtacac tgctggagtc tgcagacaaa tacccctcac ttgcagataa
     2641 ggtgacggaa gggag
//
LOCUS       DAZGHF010000281         2595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGHF010000281 DAZGHF010000000
VERSION     DAZGHF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.283"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            50..1243
                     /gene="yhiN"
                     /locus_tag="ACHK3A_005045"
     CDS             50..1243
                     /gene="yhiN"
                     /locus_tag="ACHK3A_005045"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO8742979.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGITWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(1330..2382)
                     /gene="yhiM"
                     /locus_tag="ACHK3A_005046"
     CDS             complement(1330..2382)
                     /gene="yhiM"
                     /locus_tag="ACHK3A_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFO8742980.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT          755 a          598 c          626 g          616 t
ORIGIN      
        1 ctgaggaatc tttacaattc acgcccgttt tttcttagag gagcgcaaag tggaaaggtt
       61 tgatgccatt attataggcg ctggtgcggc gggtatgttc tgttctgcgc tggcaggtca
      121 ggcaggacgc cgggttctgc tgatcgataa tggtaaaaaa ccagggcgca aaatcctcat
      181 gtctggcggt gggcgctgca actttaccaa cctttatgtc gaaccgggcg cttatctgag
      241 ccagaatccg catttttgta agtctgcact cgcgcgtttt acccagtggg atttcattga
      301 tctggtcaat aaacacggca tcacctggca cgagaaaacg ttagggcaac tcttctgcga
      361 tgactccgcg cagcagattg tcgacatgct ggtggatgag tgcgagaagg gcaatgtgac
      421 cttcagattg cgtagcgaag tgctgagtgt ggcgaaggat gaaacaggct tcacgcttga
      481 actgaacggc atgactgtcg gttgcgaaaa gctggtcatc gcgaccggtg gactgtcaat
      541 gccagggctg ggcgcgtcgc cgtttggtta taagattgcc gaacaatttg gcctcaacgt
      601 gctgccgacc cgcgcgggcc tggtgccatt cactctgcat aaaccgttgc tcgaagagtt
      661 acaggtgctg gcgggcgtgg cggtgccttc cgtgattacc gctgaaaacg gcatcgtttt
      721 ccgtgagaac ttactcttca cccaccgcgg cttatctgga ccggcggtgt tgcaaatttc
      781 cagctactgg caaccgggtg aatttgtcag tatcaatctg ctaccggatg tggacctcga
      841 aaccttcctg aatgagcagc gtaatgcaca tccgaatcag agcctgaaaa acacactggc
      901 ggttcatcta ccgaagcggt tggttgaacg tttacaacaa ctcgggcaaa tcccggatgt
      961 ttcgctaaaa cagctcaacg tgcgtgacca acaggcactg attagcacat tgaccgactg
     1021 gcgcgtacaa cccaacggca ctgaaggcta tcgcactgcc gaagtgacgc tcggcggcgt
     1081 ggacaccaac gaactctctt cacggacgat ggaagcgcgc aaagtgcctg ggctgtactt
     1141 catcggcgaa gtgatggacg tcaccggctg gctggggggc tataacttcc agtgggcgtg
     1201 gtcgagtgca tgggcttgtg cgcaggattt ggtggcagaa taagaagcaa aatgtgctct
     1261 cgataccaga gggattaacc tgaataaaac cagataaggc gtccaacgcc ttatctgaag
     1321 ccttttttat tattttttcg ctgaacccgc ttctaatata gaaacgatgg aaaacagtgt
     1381 aaaacaaact gcacctaagc caaccagaac tcgcgaagga ataaagtaag ccatatcggt
     1441 ttgcgcaatt tcagccagga acgcagcaag gaacagacaa aacagacagg taaataccgg
     1501 aatcatgggt attctgttgg ctaacgaaca ggtgcgtcgc cataccagcg ctaacaacca
     1561 cactttggag aaaatactgt agcagatcat ccccaggcaa atgagaataa tgccgggggc
     1621 caggcgggcg cttgcatctg aactgattaa tacgtagata ccaaaaataa tggtaagtga
     1681 gccaagcaaa ataacccaat agcaccacag ccaatgttct ttcactgaga acgtattccg
     1741 cgtttgatgg acaatagtgg cgacaaggcc aataagacaa gcgcaaattg ccgttaaccc
     1801 cattagtacg tgacctgcaa cataatgtgg tgtgatatct gcactacgta acagtatgac
     1861 tgaccaaatg aaaccaaaga gagtaagcag aacgggaacg gcaattaagc aattaccgat
     1921 tgttgaagaa taggcctgca atggcgtacc gtcactctta ctgcccgagg cgtttttggg
     1981 gatcaacaga aaatgtccgg aagatgccgc tacggttgat acacaagcgg caatcatgcc
     2041 aacgccgaaa ataacatgac cggcgacaaa ttcatctgcc atcacattat tgcttgccaa
     2101 cagtgaccat ccccaaatca tagttgtagc agaaccggca taaccaataa cgggaaataa
     2161 cctgttgtag aatttattca tgccgtgagt taactgagat ataataataa atgcggtagt
     2221 gaacaatgcc agacatatcg cagaaagaga aatcagaaca tgccctgcaa cgaaatattc
     2281 actttgccca gaaccggtta atacaaactc acctaatgcg atgcatatta tacccatcag
     2341 taaaggaatt aatttaaaca accatccgat atatatattc attttaaacc ccatatatat
     2401 tttctgctac acttcgaaaa acatatgatt cgtcttttca gttagttaaa tatgtctata
     2461 agatgccatc aattaaaaaa agtaacttca ctataatcgg atttagcgtt atggggatca
     2521 acttttagcg gtgagttaat gctattaaat aaatatttat cctaagataa actctgaatt
     2581 aatatcttta tctta
//
LOCUS       DAZGHF010000282         2489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGHF010000282 DAZGHF010000000
VERSION     DAZGHF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.284"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(61..285)
                     /locus_tag="ACHK3A_005047"
     CDS             complement(61..285)
                     /locus_tag="ACHK3A_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742981.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLYSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(309..776)
                     /locus_tag="ACHK3A_005048"
     CDS             complement(309..776)
                     /locus_tag="ACHK3A_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO8742982.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCHGT
                     VREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAQEYIRTQCIN"
     gene            complement(1075..1608)
                     /locus_tag="ACHK3A_005049"
     CDS             complement(1075..1608)
                     /locus_tag="ACHK3A_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8742983.1"
                     /translation="MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            1734..2054
                     /locus_tag="ACHK3A_005050"
     CDS             1734..2054
                     /locus_tag="ACHK3A_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742984.1"
                     /translation="MNNNDNNLRKEFLQIMNENVKSELKALIPDNSETTQAILSEPYG
                     MLSTETLDIIITTLTPLMLQHLKHNINKWFNDELSHPGCSWDKNFACLQKKRLFNKLS
                     LKFR"
     gene            complement(2047..>2489)
                     /locus_tag="ACHK3A_005051"
     CDS             complement(2047..>2489)
                     /locus_tag="ACHK3A_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742985.1"
                     /translation="FRHEGNDAKGSFTKIGPFFLKVDFSDQEWECNRPSFVVSGDDGK
                     ITISENGKVTPPSHQHCETLIEFAIDYLKNNKKQGLMKRIGRCMGYLQVAAEIEALVS
                     GADKDAVVRETVLRDFNTPPFKTEPDDWIQPGLTYLKGRFGAVI"
BASE COUNT          646 a          671 c          530 g          642 t
ORIGIN      
        1 tgcggtctct gttaatacgg gaatacggcg acaataccgc gccatggata ataaggtcgc
       61 tcaacacact ggctgtaatg cagccgatac catgcggcat ttagcggcat tcatcgtaca
      121 ctcaacggtt agctcttcat tcgtggcatt cacctgaaag gtccgggagt ataattgcgt
      181 acatttacca ctgaacgaac cttcaacaag aacacgacca cgctgcaaaa tacggaacgg
      241 aattgttccc tgaaaaggct ttacggttac cagtaatttc ttcatgcatt ctccggataa
      301 caaaaatact agttaataca ctgagtgcgg atatattcct gcgccccttc cagctgcttc
      361 tgcattgtca tcaaccgttc tctgaggatg aaataatccc gttcagcggt gtctgccagt
      421 cgggggccgg ttgcattatc cacgccggag gtgccggtgg cttcacgcac ggtaccgtgg
      481 caggtggcgt tgatccgcag gcgcttacga ccagcggcaa catcagcacg cagagtttca
      541 ttttcagctc tcgcatcggc taattccctc gagtattttg catcgagcgc agcaacatcg
      601 cgctggcgca cctgcatatc agtaatggtt gcgtttgcca gctccagctc tctggctttt
      661 ttatcgcgct gcgctttgta ggtgatggcg ttatcgcggt aatgattcag ccccagacta
      721 agcgcaccac aggccaccag cagggcaatg atgaccacac acagaacgcg gttcatttca
      781 ccaccagcgt atctgaccga tgaaataacc ggaggccata atcacaaaca ccagccagat
      841 aaggatgaac ttccaggtgg ataatttttc agccatcact cgaatctccc gaatcagttt
      901 gctaaaatta aacacacttt ctcctttgac ttttccggag tcaggaaaca caaaaccccg
      961 cttgctacca acaaacgggg tttttacttt tattcactta ggttttgcca gttcgcagga
     1021 tttcgtgtta tccgtccgcg ttggccaacg tcatttttca gcaaaatatt ctgcttatct
     1081 gtcgattccc cagcacgcca gcgcgctctc ctggtcacga cgggatatct ggccgtaaca
     1141 gttgtttgag cggatacggc agtctctgcc accgtcctta atccaccagc gaatcgcttc
     1201 acacgctccc ctgcgatcgc ctgcattaat tcgtttataa aacgtcgacg ggaaacactt
     1261 accggggcca atgttatacg ggcagaatga cgcaataccc gctttctggg gttcggtcag
     1321 tggcactctg atgtttttct ccacccatgc cagcgcctta tcccgttcga tagcgttaac
     1381 ccggtcgcat ttttccttcg acagcttcat gccaggaatc acaggcttac catccaccat
     1441 gatggcaccc cggcagatgg tccagatacc cgcaccatca cggtatgccg tggtgtggtt
     1501 gccttccttt tcatccagaa actggtcgag gatttcaggc gcagaagccc ctgcggcaat
     1561 cagcgccaga acggcagctg acaggccgta tttgattttt gcgttcatgg atatttatca
     1621 ggatttatag ctctcttatt cctggatatg tcagatatat aatccggtac tcacgatgaa
     1681 tataatcaac aggcaccagg acacacccgg taacaacaaa tgagatttta taaatgaata
     1741 ataatgacaa caatttacgc aaagagtttt tgcagataat gaacgaaaat gttaaatcag
     1801 aattaaaagc gcttatcccg gataatagcg aaaccactca ggccattctc tcagagcctt
     1861 acggcatgct ttcaacagaa acactggata tcattatcac cacattaaca ccattgatgc
     1921 ttcagcatct gaagcacaac attaacaaat ggtttaatga cgaactcagt cacccgggtt
     1981 gttcatggga taaaaacttt gcctgtctgc aaaaaaaacg gctcttcaat aaactatcgc
     2041 ttaagttcag ataaccgccc caaaccgccc tttcagataa gtcagccccg gctggatcca
     2101 atcatctggc tcagtcttaa agggtggagt attgaaatca cgaagaacgg tctcccgcac
     2161 aactgcatcc ttatcagcac cactgaccag cgcttcaatc tcagcggcta cctgcagata
     2221 ccccatgcaa cgaccaatgc gcttcattag tccctgcttt ttattgttct tcaggtaatc
     2281 aatggcaaat tcaatgagcg tctcacagtg ctggtgcgat ggcggtgtta cttttccatt
     2341 ttctgagata gttattttcc cgtcatcacc ggatacaaca aaggatggcc ggttacactc
     2401 ccattcctgg tcactgaaat ctaccttcag aaagaacggc ccgatttttg taaaggaacc
     2461 tttggcatcg ttcccctcat gacggaata
//
LOCUS       DAZGHF010000283         2484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGHF010000283 DAZGHF010000000
VERSION     DAZGHF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.285"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(104..1393)
                     /locus_tag="ACHK3A_005052"
     CDS             complement(104..1393)
                     /locus_tag="ACHK3A_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO8742986.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGGIGHEDRMTILEIHDCRP
                     EEHQWVDQVINVTMKAWQTSGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKAVS
                     DLSEGVVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVK
                     SWTNTQIKAAWTEIQHATDMTLDMLKQVDWVQVLTSVAKTVGVILVAVGVGGLVVTLG
                     IPEAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVG"
     gene            complement(1393..>2484)
                     /locus_tag="ACHK3A_005053"
     CDS             complement(1393..>2484)
                     /locus_tag="ACHK3A_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO8742987.1"
                     /translation="FANSKLRMEDRRGEEHIKLATEYGKTQLNSGHLVDSQGQLRGKG
                     TELRTDEWGTLRAGKGLFVAADAQAKAQGEALDMDAALKEIDRLNQQLQQLENAAERA
                     QALKADIDSQIQMFAQRLKPLNEAVLFSAPEGIALTSGEDMQMSAARNVAVNAGGDIS
                     TGTMGNLTALAGERLGLFARTGQLSVKSGEGPVEVQAQNGSMRLFAEKKLTISSESDI
                     SFTGKKRITLIGGGSYLKLEAGKVEYGTMATCMRRVKRTMTAAAQSMPMAFPRMSSSH
                     IYSAIYRLLDKHGNILVNTPCRLTTPSGQTVTGYSDNEGRSVPVYTRQQEDVELHFFV
                     KKPQPEETMWFVGETDTQQLETELRESLP"
BASE COUNT          494 a          759 c          606 g          625 t
ORIGIN      
        1 gattgactgc cggactgccg tctttgtatt tcacggaggc ttccggcagc catttctcaa
       61 tgtagttcag ctcaaaatca tgcaattcca tacctgctcc tgtttaacca actgccagta
      121 ccgccgtacc cgtaccgaga atcgtcgcca gtgttcccca ggcgcttatt gccagacgaa
      181 caatcagtaa aaacgcgcca ataatggctt ccgggatacc cagcgttacg accagtccgc
      241 cgacacccac agcaaccaga ataacgccga ccgttttcgc gacactggtt aaaacctgta
      301 cccagtccac ctgtttcagc atatcaagcg tcatatcggt ggcgtgctgt atttctgtcc
      361 atgcggcctt aatctgggtg ttggtccagg atttcaccga cttaccggtt tggctggccc
      421 agacccagac ctcacttccc tgccggtaaa cccactcacc gctgtcgtta agccagctgg
      481 ctgctgaatt caggaccagt tgcgtctctt ttgacagggc attccagcct tcagccacac
      541 cacgtaccac accctcactg agatcagata ctgctttacc ggcgtaagtc cagggctcta
      601 ttctggcagc aacgggggtt gatggcggac ggggcataaa gaccggcggg tagagagcca
      661 gcggccagta tttcgcgccg ctggtctgcc aggctttcat cgtcacgttg atcacctgat
      721 caacccactg gtgctcttcc gggcgacagt cgtggatctc cagaatggtc atgcgatcct
      781 catgaccaat tccaccgaca atctgcatat actcttcata ttggtcttca tacagcctat
      841 ctcccggaaa cttcacctcc accaggcggg ccaggttatc gctgtgcata ttgccttcgg
      901 tatccggccc cgccagtccg ggccagcggt cctccttatt tttaacaata atcacgtccg
      961 ggcggcggta aatgcccctg ccgggcacag ggaaaggatt ggtcgtgtgg cgacggccag
     1021 caccgtcttt ttccagcagc gtactgctca gaaaagggcg tggtggcgtg gtgcgcatat
     1081 caaacgccac ctccgcctta taataccagc agtaattgcg ggcgatttca tccacacgaa
     1141 taagaccact cataacggtc tgcttaaggc tcatctggta agttttacct ttccggcgga
     1201 tttgtgccga cagttgcgga aaacgcatgg cgtattccac ctttatatgc aggtactgac
     1261 ggttttccgt ggttggcaga cgcgactgct tttcgaccat ttcgacaccc gtcgtagtgc
     1321 agtcccacgg ctggttgttc gccccggtgg gaatatcaga tgtcggtgtc tcgggtaaat
     1381 cgggcatttc cattatggca ggctctccct gagttcggtt tccagttgct gcgtatcggt
     1441 ttcccccaca aaccacatcg tttcttcggg ttgtggtttt ttaacaaaaa aatgcagttc
     1501 cacatcctcc tgctggcggg tgtaaaccgg cacggagcgg ccttcgttat cgctataccc
     1561 cgttactgtc tgacctgatg gagtggtgag gcgacagggg gtattgacca gaatattgcc
     1621 gtgtttgtcc agcaacctat agatagccga gtagatatgc gaagaactca tacgggggaa
     1681 tgccatcggc atactctgcg cagccgccgt catcgtccgc ttcacccgac gcatacaggt
     1741 tgccatcgtg ccatattcaa ccttcccggc ttccagtttc aggtaactgc caccgccaat
     1801 cagcgtaatg cgtttcttgc cggtaaacga gatatcactt tctgacgaaa tggtcagctt
     1861 cttctcagca aacaaccgca tgctgccgtt ttgcgcctgt acctctactg gcccctcccc
     1921 ggatttgacg ctcagttgtc cggtcctggc aaaaagcccc agcctttctc cggccagcgc
     1981 ggtcaggttg cccattgttc cggtactgat atcacctccg gcgttgacag ccacatttcg
     2041 tgctgcactc atctgcatat cttcaccgct ggtcagggca atgccttccg gagccgagaa
     2101 aagcacggct tcattgagcg gtttcagccg ctgcgcaaac atctgtatct gactgtcaat
     2161 atcagccttc agcgcctgtg cccgttctgc ggcattttcc agttgctgca gttgctgatt
     2221 aagcctgtcg atttccttca gcgcggcatc catgtccagt gcctcgccct gtgctttcgc
     2281 ctgcgcatcc gcagcaacaa aaagtccctt ccccgctctc agggttcccc actcatcggt
     2341 acgcagctct gtgcctttac cccgcagttg cccctggctg tccaccagat gtccgctgtt
     2401 aagctgggtc ttaccgtact ccgtggcgag tttgatatgc tcctcgccgc gccggtcctc
     2461 catccgcagt tttgagttag cgaa
//
LOCUS       DAZGHF010000284         2478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGHF010000284 DAZGHF010000000
VERSION     DAZGHF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.286"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(104..1387)
                     /locus_tag="ACHK3A_005054"
     CDS             complement(104..1387)
                     /locus_tag="ACHK3A_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO8742988.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGRNRTRMTILEIHDCRPEE
                     HQWVDQVVNVTMKAWQISGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKVVSDL
                     SEGAVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVKSW
                     TNTQIKAAWTEIQHVTDMTLDMLKQVDWVQVLTNVAKTVGVILVAVGVGGLVVTLGIP
                     EAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVS"
     gene            complement(1387..>2478)
                     /locus_tag="ACHK3A_005055"
     CDS             complement(1387..>2478)
                     /locus_tag="ACHK3A_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO8742989.1"
                     /translation="PANNKLRMEDRRNEEHIKLATEYGKTQLNSGHLVDSQGQLRGKG
                     TELRTDEWGTLRAGKGLFVAADAQAKAQGEALDMDAALKEIDRLNQQLQQLENAAERA
                     QALKADIDSQIQMFEQRLKLLNEAVLFSAPEGMALTSGEDMQMSAARNVAVNAGGDIS
                     TGTMGNLTALAGEKLGLFARTGQLSLKSGEGAVDVQAQNGSMRLFAEKKLTISSESDI
                     SFTGKKRITLIGGGSYLRLEAGKVEYGTMATCMRRVKRTMTEAAQSMPMTFPRMSSSH
                     IYSAIYRLLDKHGNILANTPCRLTTPSGQTVMGYSDNEGRSVPVYTRQQEDVELHFFV
                     KKPQPEETMWFVGETDTQQLETELRESQS"
BASE COUNT          487 a          758 c          602 g          631 t
ORIGIN      
        1 gattgactgc cggactgccg tctttgtatt tcacggaggc ttccggcagc catttctcga
       61 tgtagttcag ctcaaaatca tgcaattcca tacctgctcc tgtttaacta actgccagta
      121 ccgccgtacc cgtaccgaga atcgtcgcca gtgttcccca ggcgcttatt gccagacgaa
      181 caatcagtaa aaacgcgcca ataatggctt ccgggatacc cagcgttacg accagtccgc
      241 cgacacccac agcaaccaga ataacgccga ccgttttcgc gacgttggtt aaaacctgta
      301 cccagtccac ctgtttcagc atatcaagcg tcatatcggt gacgtgctgt atttctgtcc
      361 atgcggcctt aatctgggtg ttggtccagg atttcaccga cttaccggtt tggctggccc
      421 agacccagac ctcacttccc tgccggtaaa cccactcacc gctgtcgtta agccagctgg
      481 ctgctgaatt caggaccagt tgcgtctctt ttgacagggc attccagcct tcagccacac
      541 cacgcaccgc cccctcactg aggtcagata ctactttacc agcgtaagtc cagggctcta
      601 ttctggcagc aacgggggtt gatggcggac ggggcataaa gactggcggg taaagagcca
      661 gcggccagta tttcgcgccg ctgatctgcc aggctttcat cgtcacgttg accacctggt
      721 caacccactg gtgctcttcc gggcgacagt cgtgaatctc cagaatggtc atgcgggttc
      781 ggttcctgcc cacaatctgc atatactctt catattggtc ttcatacagc ctatctcccg
      841 gaaacttcac ctccaccagg cgggccaggt tatcgctgtg catattgcct tcggtatccg
      901 gccccgccag tccgggccag cggtcttcct tatttttaac aataatcacg tccgggcggc
      961 ggtaaatgcc cctgccgggc acagggaaag gattggtcgt gtggcgacgg ccagcaccgt
     1021 ctttttccag cagcgtactg ctcagaaaag ggcgtggcgg cgtggtgcgc atatcaaacg
     1081 ccacctccgc cttataatac cagcagtaat tgcgggcgat ttcatccacg cgaataagac
     1141 cactcataac ggtctgctta aggctcatct ggtaagtttt acctttccgg cggatttgtg
     1201 ccgacagttg cggaaaacgc atggcatatt ccacctttat atgcaggtac tgacggtttt
     1261 ccgtggttgg cagacgcgac tgcttttcga ccatttcgac acccgtcgtg gtgcagtccc
     1321 acggctggtt gttcgccccg gtgggaatat cagatgtcgg tgtctcgggt aaatcgggca
     1381 tttccattat gactggctct ccctgagttc ggtttccagt tgctgcgtat cggtttcccc
     1441 cacaaaccac atcgtttctt cgggttgtgg ttttttaaca aaaaaatgca gttccacatc
     1501 ctcctgctgg cgggtgtaaa ccggcacgga gcggccttcg ttatcgctat accccattac
     1561 tgtctgacca gatggagtgg tgaggcgaca gggggtattg gccagaatat tgccgtgttt
     1621 gtccagcaac cgataaatag ccgagtagat atgcgaagaa ctcatacggg ggaatgtcat
     1681 cggcatactc tgcgcagcct ccgtcatcgt ccgcttcacc cgacgcatac aggttgccat
     1741 cgtaccatac tcgaccttcc cggcttcaag ccgcaggtaa ctccccccgc caatcagcgt
     1801 gatgcgtttc ttgccggtaa acgagatatc actttctgac gaaatggtca gcttcttctc
     1861 agcaaacaac cgcatgctgc cgttttgcgc ctgaacatcc actgcccctt ccccggattt
     1921 caggctaagt tgaccggtcc gggcaaaaag ccccagcttc tctccggcca gcgcggtcag
     1981 gttgcccatt gttccggtac tgatatcccc cccggcgttg acagccacat ttcgtgctgc
     2041 actcatctgc atatcttcac cgctggtcag cgccattcct tccggtgccg agaaaagcac
     2101 tgcttcattg agcagtttca gccgctgctc aaacatctgt atctgactgt caatatcagc
     2161 cttcagcgcc tgtgcccgtt ctgcggcatt ttccagttgc tgcagttgct gattaagcct
     2221 gtcgatttcc ttcagcgcgg catccatgtc cagtgcctcg ccctgtgctt tcgcctgcgc
     2281 atccgcagca acaaaaagtc ccttccctgc tctcagggtt ccccactcat cggtacgcag
     2341 ctctgtgcct ttaccccgca gttgcccctg gctgtccacc agatgtccgc tgttaagctg
     2401 tgtcttgccg tattccgtag cgagtttgat gtgctcctca ttgcgtctgt cttccatccg
     2461 cagcttgttg ttcgcagg
//
LOCUS       DAZGHF010000285         2457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGHF010000285 DAZGHF010000000
VERSION     DAZGHF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.287"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1603
                     /gene="espI"
                     /locus_tag="ACHK3A_005056"
     CDS             <1..1603
                     /gene="espI"
                     /locus_tag="ACHK3A_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFO8742990.1"
                     /translation="VNSDDAAFLPTINMADGGFKLMSDSSVLKARDQASVVGDIISDK
                     QATISFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAIHAPSASATVNNTWWQLTG
                     DSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAYAMRTDLKNADKLVVNQKL
                     SGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQAIGFSNVTPVITAIDAG
                     DKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNLNKRMGDLRDINGEAGAWA
                     RIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADAFKGKTK
                     SVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWYAGAEAGYRC
                     HVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDYNPLIGRTGVDVGKSFSGK
                     DWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSVGLNAEIRDNVRFG
                     LEFEKSAFGKYNVDNAVNANFRYSF"
     gene            2136..2456
                     /locus_tag="ACHK3A_005057"
                     /pseudo
     CDS             2136..2456
                     /locus_tag="ACHK3A_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          678 a          582 c          625 g          572 t
ORIGIN      
        1 agtgaattca gatgatgctg cttttttacc gaccatcaat atggctgatg gcggatttaa
       61 actgatgtct gattcatcag tactgaaagc cagagaccag gcatctgttg ttggtgatat
      121 tatttctgat aaacaggcca caatcagctt cggaactgaa tcaggtaaag agggcatatt
      181 gtctgagaag gcatcccggg gactcgcggt aggattactg agtggtttta atacggcata
      241 ccgcggtgca attcatgccc cgtcagcatc tgccactgtg aacaacacct ggtggcaatt
      301 gacaggagac tcctcacttc gctcgttaaa aaataccgga agcatgacat attttacagg
      361 aagtgcagcg aataaagcat tccatacact gacggttgat gagctgacga cgaatggcac
      421 tgcgtatgcc atgcgtacgg acctgaaaaa tgcggataag ctggtagtaa accaaaagct
      481 gtcaggtaag gacaatattc tgctggttga ttttctgaac aaacccaccg gagaaaaact
      541 ggatattgaa ctggtgagtg caccggggaa cagcagtaag gatgttttta aaggaagtga
      601 acaggcaata ggtttcagca atgtcacgcc tgttattaca gctatagacg ccggagataa
      661 aacaacatgg aacctgaccg ggtacaggat ggcagaaaat cctgccgcaa cccaaagtgc
      721 ctcaggcctt gcatctgtgg ggtacaaatc atttttgagt gaggtcaaca acctgaataa
      781 acgtatgggt gacctgcgtg acatcaatgg tgaagctggc gcatgggcac gtatcatgag
      841 cggaaccggc tctgccggtg gtggtttcag tgacaaccac acacatgttc aggtcggtgt
      901 cgacaaaaaa catgagctgg acggactgga tttgtttacc ggcttcactg tcacacatac
      961 tgacagcagt gcctctgctg atgctttcaa aggtaaaaca aaatctgtgg gggccggact
     1021 ctatgcttcc gccatgtttg attccggtgc ctatatcgac ctgattggta agtatgttca
     1081 tcatgataat gagtacaccg caacctttgc cggactcgga acccgtgatt acagtacgca
     1141 ttcatggtat gccggtgctg aagcaggcta ccgctgtcat gtcactgagg atacctggat
     1201 tgagccacag gcagaactgg tttacggtgc tgtatccggt aaacagtttg catggaagga
     1261 ccaggggatg catctgtcca tgaaggacag ggactacaat ccgctgattg gtcgtaccgg
     1321 tgtggatgtg ggtaaatcct tctcaggtaa ggactggaaa gtgacagccc gtgccggtct
     1381 gggctaccag ttcgacctgc tggctaacgg cgaaaccgta ttgcgggacg catcaggtga
     1441 aaaacgtatc aaaggtgaaa aagacagccg tatgctgatg tccgttggcc tgaatgcaga
     1501 aatcagggac aacgtccgct ttggactgga gtttgagaaa tccgcctttg gtaagtacaa
     1561 cgttgataat gctgtcaacg ctaacttccg ttactcgttc tgatactccg tattcaaaag
     1621 ggcctgaggg ccctttatgc tcaggacctg ccgcccgggt atacatttcc ggagtatcag
     1681 tcgatttgtt tacttaaccg ccatgaccaa agccggcagt aatgtcggct ttttacgtcc
     1741 atatattcca ctcatccgga actgtgacac agaacctgaa tacaggattg atcaccggtg
     1801 cccacaaatg gcacattttc agtaagattc tgaatgttac attccccctt catgtgtgac
     1861 acagctgtca cagagaacga aacggaaagc acatgaaaaa cataaaaact cacaccggac
     1921 tccttatcca caaagaacaa acacggcgcg ttcgtcttca tgaaacaccg gccgcctggt
     1981 gccacacacg ccgggaatgc tacagcaaaa cgaccggtcg tcgctgcggc agccctgaca
     2041 gtctcagcag gctgattctg agcagcattc agcccataga atgagataac ccgtatgaat
     2101 gatgacagac gtctacggta cccgatgtat ggaacatttg ctttctggct aacggcatgc
     2161 ccgacacatg gttttacacc gatgacgcct ccgtcacaaa agagtggggg cgggaatatt
     2221 gtgacaatgt ggaagtgttc cctctgtgct atatgcatga cggtcccgtt ttccgggggc
     2281 gctatattga tgagctcagc gccgcacgga acagaatgct ccatatgcac cacatactgc
     2341 cggaatgaac ccggtacaga gaaaattttc atgacgcaga atgtatccgt gaaattcacg
     2401 aagcgcccct gcaggctccc ggcattctgc aaaaatacaa acgcacaaaa tcataag
//
LOCUS       DAZGHF010000286         2455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGHF010000286 DAZGHF010000000
VERSION     DAZGHF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.288"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..373)
                     /locus_tag="ACHK3A_005058"
     CDS             complement(<1..373)
                     /locus_tag="ACHK3A_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742991.1"
                     /translation="MTGLSEQAKELHMMMGRRKPPKIQAVLNTRYWKNTEKTIKRTVG
                     MNIHTLSQWCGEQYEKMFISKVAEVYMLHHTAAILRRKGVYVDVFKIPSKIMNPPEAR
                     ANCTGCPSSQNVSPFFAYSEPN"
     gene            487..1371
                     /locus_tag="ACHK3A_005059"
     CDS             487..1371
                     /locus_tag="ACHK3A_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO8742992.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICTTHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRHQELSLATVTVRVATLFPSSFPVLPVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGETIGKSFDVAVARFSFPAWMLQLLR
                     KARDRIIRLLVTLWDHLF"
     gene            1490..2167
                     /locus_tag="ACHK3A_005060"
     CDS             1490..2167
                     /locus_tag="ACHK3A_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8742993.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            2173..2411
                     /locus_tag="ACHK3A_005061"
                     /pseudo
     CDS             2173..2411
                     /locus_tag="ACHK3A_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
BASE COUNT          604 a          636 c          575 g          640 t
ORIGIN      
        1 tgttaggctc tgaataagca aaaaaaggag agacattctg agaagaaggg caaccagtac
       61 aattggctct cgcttcaggc gggttcatta tttttgaggg aatcttaaaa acatccacat
      121 aaactccttt acgcctcaat attgctgccg tatggtgaag catatagact tctgccactt
      181 ttgaaataaa catcttctca tattgttctc cacaccactg actgagcgta tgaatattca
      241 tacctacagt tctttttatc gttttttctg tgtttttcca gtatcgggtg ttaagaacag
      301 cctgtatctt cggcggtttt ctccgcccca tcatcatatg aagttctttt gcctgctcag
      361 ataatcctgt catttattac ctccgaatat attccattaa aaatggaaat aattatacgc
      421 ccatattacc tgaatgataa accggattac tgtgatccgg ttcagtgaaa tcaacaggat
      481 accggtatgt cattcagcca ttcttccctc tctgcacaag tgaaatcata tctgacgttt
      541 cttcctgaag aaattcgcca gaaaatcctt gaacatctcc acggtgttat tcattacgag
      601 cccgtgattg gcattatggg taaatccggc accggcaaaa gcagtctgtg taatgccatt
      661 tttcagtccc gtatctgcac cacgcatccc ctgaacggct gcacccgcca ggctcatcgc
      721 ctcaccctgc agattggtga acgcagaatg acgctggtcg atctgcccgg cattggtgaa
      781 acaccgcagc atgatcagga ataccgggag ctttaccgtc agttactgcc ggaactggat
      841 ctgattatct ggatcctgcg ggctgatgaa cgtgcgtatg ctgccgatat tgccatgcat
      901 cagtttttac tgaatgaagg tgcagatccc tcgcgttttc tgtttgtact cagtcatgcc
      961 gatcgcgtgt ttcctgctga agaatggaac gacacagaaa aatgcccgtc ccgtcaccag
     1021 gaactctcac tggcaactgt tacagtccgg gtggcaaccc tgttcccgtc atcatttccg
     1081 gtactccccg ttgctgcgcc tgtcggctgg aatcttccgg cgtttgtgtc actaatgatc
     1141 cacgccctgc caccgcaggc aaccagtgcg gtttattcac atatccgggg ggaaaaccgc
     1201 tctgaacagg cccggaaaca cgcacaacag acttttggtg aaaccatcgg gaaaagtttt
     1261 gacgtcgccg ttgcccgttt cagttttccg gcctggatgt tacagcttct gcgtaaagcc
     1321 cgggaccgca ttatccgcct gctggtcaca ctgtgggatc acctgttctg agcgccccgg
     1381 agtcccccgt ggcaaccgta tacctttaca cgacaacgga ttacggtaac cactgccgta
     1441 taccgtctgt cattatcaat aacacactct ttaatttcag aggtcagtca tgaataccag
     1501 aattcttttt cccctgctgt tcactgtggc atccttctcc gcctccgccg gtaactgggc
     1561 tgtcaaaaac ggctggtgcc agaccatgac ggaagatggt caggcgctgg taatgctgaa
     1621 aaatggcacg attggtatta ccggcctgat gcagggatgc ccgaatggcg tacagacgct
     1681 cctgggcagc cgtatcagta ttaacggtaa cctgatcccc acatcacaaa tgtgtaatca
     1741 gcagacggga ttcagggctg ttgaggtgga aatcggacag gcgccggaaa tggtcaaaaa
     1801 agccgttcac tccatagcag agcgtgatgt gtccgtttta caggcatttg gtgtacgaat
     1861 ggaattcacc cgcggtgata tgctgaaggt ctgtccgaaa tttgtcacat cacttgccgg
     1921 tttttccccg aaacagacga ccactattaa taaagactcc gtcctgcagg ctgcccggca
     1981 ggcatacgcc cgggaatatg acgaggaaac aacagaaacc gctgattttg gctcttacga
     2041 agtaaaaggc aataaggttg agtttgaagt attcaatcct gaagaccgtg cgtacgacaa
     2101 agtgaccgtc acggttggtg ctgacggtaa tgccaccagt gccagcgttg aatttatcgg
     2161 aaaatagccg gtatgtcgga ctgtcaccct gttttattgc ccgaaggccc tttctcacgc
     2221 aaacaggcaa tggctgccac aacagcttac cttaccgcaa tgtgcttatt gaagatgacc
     2281 atggaacgca tttcctgctg gttatccgca atgccgaagg gcagctacgc tggcggtgct
     2341 ggaattttga gtctgacgcc ggaaaacagc ttaatgtgtg gctcgccagt gaagggcttc
     2401 tcagacaata aaagtcttca tttcatccat caggccgcgt cttctccggg agacg
//
LOCUS       DAZGHF010000287         2437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGHF010000287 DAZGHF010000000
VERSION     DAZGHF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.289"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1076
                     /locus_tag="ACHK3A_005062"
     CDS             <1..1076
                     /locus_tag="ACHK3A_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO8742994.1"
                     /translation="SPSIRPSLYAYGANATTLKPFSVALGAGSVADRGAGIEAYMPET
                     KRWNPGDVVKSRMSDKQLNRLMEIDKLISAYNSQETLSAEETKQLEEVKKERADMFAT
                     YIATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQTVTEKADAAL
                     DKRITNNTSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAEEHKLLISENSK
                     QISTNTLGIRENTQRVQDEGQARIRGDRETLRAATGYTDMRVNGLERNTNKRFAQLKE
                     DIDKNRKRADAGIAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAAGFHFNTSENSA
                     VKVNAAWDTENGAGVSAGMALGW"
     gene            complement(1506..2081)
                     /gene="dolP"
                     /locus_tag="ACHK3A_005063"
     CDS             complement(1506..2081)
                     /gene="dolP"
                     /locus_tag="ACHK3A_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO8742995.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADIASRVSGVKRVTTAFSCIR"
BASE COUNT          698 a          571 c          596 g          572 t
ORIGIN      
        1 catccccgtc aatacggccc tcgctgtacg cttacggtgc aaacgcaaca acattgaaac
       61 cattctctgt cgctctgggc gcaggaagtg ttgctgatcg tggcgctggt attgaagcat
      121 atatgccaga gacaaagcgc tggaatccag gggatgtagt aaaatccagg atgtctgaca
      181 agcagcttaa caggcttatg gaaatcgata agctaatatc cgcttataat tcacaagaga
      241 cgctgtctgc tgaagagact aaacaactgg aggaagtgaa aaaagagcgg gctgatatgt
      301 ttgcgacata tatagcaacg cacgatgctg tatctgtagg tgttggtgca acgacaacag
      361 gacgacatgc tgttacaaga cagataacta gcgttgcagc aggttctgtc gatacggatg
      421 ctgttaacgt tgcacagctc agggatttgc agactgtgac agaaaaggca gatgcagcgc
      481 ttgataaacg cataacaaac aacaccagtg cgataactaa aaatctgcag gtggttaaca
      541 gtaataaaga ggaaatcagt aacattaata aaacgacaaa aattctggat gaaagagtga
      601 cagccaatac acagacagca gaagaacata agctgcttat ttcggagaac agcaaacaaa
      661 tcagtaccaa cactcttggt atcagagaga atacacaacg ggtccaggat gaaggacagg
      721 ctcgaatccg gggagacagg gaaacactgc gtgcagcgac cggttatacc gacatgcggg
      781 taaacgggct ggagcgtaac acgaataaac gttttgcaca gcttaaagag gatatagaca
      841 aaaacagaaa acgtgcagat gcaggtattg caggtgcgat ggcaatgacg gggataccgt
      901 ccgtccccgg gaaaaaggtc tcatttggta tggccatggc cggataccgg agtgaaggtg
      961 ctgttgctgc cggcttccat tttaatacaa gtgaaaacag tgctgttaaa gtgaatgcag
     1021 cctgggacac tgaaaatgga gctggcgttt cagccgggat ggctctggga tggtaatgct
     1081 tccgggccag aagccgttcg cagatattgt tatccagggg acactgcacc accattcagg
     1141 aagcgattca gtttatcgca tcggctcagg atgtaattaa tacggacaat ctggctcctg
     1201 caccggagcc agacgtacac gtgtcaacgc cacgatcttt gacctgtatg gataaaagct
     1261 cagattattt accagcagcg acatccggag agcaccggga ttttatctgt gagtgaacat
     1321 acccgggcaa aaaaatcccc cgaattcagg ggggtgtcaa agaggaatta tgtatattgg
     1381 ttattataat gagcacgtct ccggtattcc gcgacgaatg ttcacgtggt ggcatgaacg
     1441 gcccggaaac gtgctcttct gatttttaac gtaaatttgc actaaaggca actcagatac
     1501 ggccttcacc gtatacagga gaatgccgtc gtgacccgct tcacgccact caccctactg
     1561 gcaatatctg ctgccgctcg cccttcctgc tctgtaacca ggcccagcaa aaagacttcg
     1621 ctgttttctg tcaccacctt gacactggat gatttgacac gggcacttgt gagtaactga
     1681 gaacgaattt ttgtcgtgag ccaggcatca cctgccatta tgaccggact gacgggagtc
     1741 ccctgccgga tttcattata gacctcctga acaccatcga caccgagtgc tatctgacgg
     1801 gcacgggcag ataattcacg gtccggagac tgaccgatca gcagcacgcg tccctgatat
     1861 gcaatctcac tgatgcgtgc ttcttttctg atttgttcat cttttgaaag tgcactgctg
     1921 accctcagct ccagcacttc atcatccacc tgcgttccga cactgcgtgg gtcggttgct
     1981 gcctttgtcc cgacagcggc tgtacccacc acagttgcag caacgcatcc ctgtaacaat
     2041 aaggctgaaa tcaggacaac agcagaatat aataaacgca tatctccccc ggaatttatc
     2101 gtacaaaaag tttaccttca gtgaaacaaa acaagctacg gactgttatt ctcttccggc
     2161 acagccggaa acagcggata cgttacgtga catatctgta tctgccagac ggaaatgttt
     2221 atgacggata tggatgtact gaaatttaat attgttcatg tttttgcacg aaaacgttga
     2281 tagcgtagta aagattctgt tttttaacta cctggtttgc tgagcaggat ctccttttcg
     2341 ttatacacag gctaaaaggc accgtggccc cccggtgcct ttttcataaa acagagaaaa
     2401 cacggctttg aatataccgt ttatgattac cgttcgc
//
LOCUS       DAZGHF010000288         2421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGHF010000288 DAZGHF010000000
VERSION     DAZGHF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.290"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..740)
                     /locus_tag="ACHK3A_005064"
     CDS             complement(<1..740)
                     /locus_tag="ACHK3A_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO8742996.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYSRQHETGGDAIYCGLYGHHIKNGVTYSPINTDFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEDNNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNR"
     gene            complement(822..1850)
                     /locus_tag="ACHK3A_005065"
     CDS             complement(822..1850)
                     /locus_tag="ACHK3A_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HFO8742997.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVKIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSEVIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFGQ"
     gene            complement(1876..>2421)
                     /gene="rfaY"
                     /locus_tag="ACHK3A_005066"
     CDS             complement(1876..>2421)
                     /gene="rfaY"
                     /locus_tag="ACHK3A_005066"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO8742998.1"
                     /translation="IDTKYGRYVFKVFAPKTKRNERFFKSFVKGDYYQNLIVETDRVR
                     SAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKL
                     HALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYG
                     YYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          764 a          478 c          395 g          784 t
ORIGIN      
        1 ttccggttac ctttgtataa ctgcatcaca gaagagtaga ttctttcgtg cgcaaatgac
       61 tcatgtcgat aatgagtgat ggttctacat gagttcatta aggaattttc tctatagtta
      121 tgcttataca tataatatgg aaccgcatac atatcgcatg taattagcag tcgatagata
      181 aaatccagat cttctcctag tcgtaaatct tcatcaaaaa aaagattatt atcttccagg
      241 agttgttttc ttataaacaa gcaccctata tggaatctga cctttttata aaaaaaatcg
      301 aaaagtaaag acccttcatt aaaatctgta tttataggtg agtaagtaac accatttttt
      361 atatgatgtc cataaagccc acaataaatc gcatcccctc ccgtctcatg ttgtctggaa
      421 tacatcttca gcagatagtt aggatctatc tcatcatcat catccataaa acatataaac
      481 tgtccttgcg ccttgcggat acctgtattc ctggcagacg aaacaccgcc attttcttta
      541 ttgataataa ttaatcgaga gtgggttaac ccatactctc tcaaaacatt tgatgtgtta
      601 tctttagaac catcattaac aataacaatt tcaaaatttg ggtaagtttg tttttctaat
      661 ttcgttagag tttcagtaat gtaatcagat gaattatacg tcggtattat tatggatact
      721 aaagggtaat cattgctcat cgtgttctct tgcctgaact aataaaatat gatagtactc
      781 atccttaatt attattgtaa ctcagacatc catgattttt attattgccc aaatagtttt
      841 cttttatact tatttaattt gaatttaatg aagtaaataa aagcgttaaa cgtatcacca
      901 tttttctttg catgttggta tttcacttga tactgctttt cattcgtggc tggaataaac
      961 gccacatcat tccagcaaga agcctgataa gcctcattaa aatacttctg gcagggataa
     1021 ttcgtccagt cgttccaggg cttcgttacg ccaacataat gaatcagctt agtatcatcg
     1081 gtaatggtct ttttataatc ttcatcgctt ttatttttta actcatagtc aatgccataa
     1141 aagcaatcat aatcgccgct aataaaaata caatgaccaa agaacagaat atttaaaata
     1201 tcctgatcaa ggcatttata tttcgtgtgc tgcgggtcac ttgccagcat agcaatggct
     1261 ttattcataa aatcattttt cgcccatgct tcaagctgca gatatactac accggcgttg
     1321 aaataattac ctggaagtcc ctctatagcc agacgcttcg ctggtttttc ctgcataaaa
     1381 ggcacatctt taataacagc tgcgaactta tcgccatcaa aattaatatc aagcaatcca
     1441 gctaatgagc ctttacagat gacatcagca tcgatatata acagattttc aataacttca
     1501 ctcagatact ggaacgcaac taaacggaag aatgttgcat aagaccactg tttcaggctg
     1561 gggaagatat caataaaggc actggagact ttataaattt ttacttcaac gttagcattt
     1621 ttgctggtaa aagcgttaac acgttgtaga taatcttcat caatgtagtc ggtaaagaga
     1681 tgaaacttaa ccgcaaggtc cgggttatgc ataacaactg attgcataga tactgccgca
     1741 ccaaataaaa aattacggtc aataccgtaa gcgatattta acacacgatc ggtgttagca
     1801 atttccccag agacagcgac ttcagtaata ctctcagcta ataaatccat gttggttccg
     1861 ttttgactgt gtggtttacg ctttgccttt taatttttta ataaatttgc gtagtttggt
     1921 tctgtagatt actgaatagt agccataatc ttttatttcg ttggcaatac ccaggtgacg
     1981 ttccatggcc agacgatcac gagctttacg ctctgccgta cagcttttac cggataggtc
     2041 aattatcctg acaccatctt tactaacgat aaagttaccc cggtgagggt cgcccgatag
     2101 catatttaag gcatgcaatt tttccattga agccttaatt tctgctttaa cattttctgg
     2161 aataatcggc atatcattga gttctacgcc ctcgacatac tcgataagca tgataaaaac
     2221 actggcatag ttgaaaattt tacgctcagc cagaaaataa aaatcattag gaaaagtaag
     2281 acctgcactt ctgacacgat cggtttcaac gatcaaattc tgatagtagt caccttttac
     2341 aaaagattta aaaaaacgtt cattcctttt cgttttggga gcaaagactt taaaaacata
     2401 gcgaccatat ttcgtatcaa t
//
LOCUS       DAZGHF010000289         2418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGHF010000289 DAZGHF010000000
VERSION     DAZGHF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.291"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            162..1301
                     /locus_tag="ACHK3A_005067"
     CDS             162..1301
                     /locus_tag="ACHK3A_005067"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO8742999.1"
                     /translation="MSTMKPAIFSFFSGSGFLDLGFENAGYEVVFVNEFHPPFMDAYK
                     HSRKKLGKQPPRFGYAQESIETIEHSVIKHSMKTLKGENRLVGFIGGPPCPDFSVAGK
                     NKGKDGENGKLSQSYIDLIIEMMPDFFLFENVKGLYRTLKHREFFEQIKNELASVGYI
                     LHERLINSIEYGAPQDRERIILVGFLDKSLAKNFEWSGQIYPNKEAFQFPWPTTNTYE
                     EDSSMECPQGIPEELTIEYWFEKNDVTNHPNAKHVFTARAGLARFQSVEEGDDSKKSY
                     KRLHRWRYSPTAAYGNNEVHLHPYKPRRITVAEALAIQSLPKNFELPGDMTLSNMFKT
                     VGNGVPYIAALGIAQSILQHLGYSNEDNSIESGSGDFELTQKYAV"
     gene            1237..2310
                     /locus_tag="ACHK3A_005068"
     CDS             1237..2310
                     /locus_tag="ACHK3A_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO8743000.1"
                     /translation="MRITASNLVQAISSLPKNTLFNYINDRNSGKIEIVRIQHPEGPI
                     EIKRFDPKKGQTQATAKTESISTQMLWRLANALEENRPVNVERVFGASYNTRSVLESL
                     LAHTPEFYWCRPARLEIMNAQKSIKPGHKHLIFLPDMPHTNGLLIEHQTNIVVSEMSF
                     DVVHQSVDIETIKPTKGMTIEEKRRHAQIQIALVKIGYCLGLDTWVAANDRSLQYNGK
                     AIAQMDGVISSLSDEQVLQSYNDAIKDARLIDCIWFREGRMMPAVMEIEHSTGIKSGL
                     TRMKQFYDHAPRLQDIRWTIVAPDEYRAKVIEFANMPQFIELKAKFFPYSSVEELYSL
                     CDRRKPKGINDLFLDSFMEDCVI"
BASE COUNT          815 a          452 c          476 g          675 t
ORIGIN      
        1 taactttata gcaatgaata ctatctaatc tgaagtagtg ccaggtctta tttgctaata
       61 tattaaaaag taaattgata attaacaagt tatcatgtaa aatagtagga tcgtacagcg
      121 agatatttag taaaaattta agctaacttg ataggtataa aatgtccaca atgaaacccg
      181 ctatattttc attcttttca ggatcaggtt ttcttgacct aggcttcgaa aatgccgggt
      241 atgaggtggt tttcgtcaat gaattccatc ctcccttcat ggacgcgtat aaacactcaa
      301 gaaaaaaact tggcaaacag ccgccacgct ttgggtatgc ccaagaaagc attgaaacta
      361 ttgagcattc agtgattaag catagcatga aaacactgaa aggtgaaaac cgcctagtgg
      421 gcttcattgg cggaccacca tgccctgact tttctgttgc tggcaaaaac aaaggcaaag
      481 atggtgaaaa tggcaaatta tcacaaagtt acattgacct gataattgaa atgatgcctg
      541 atttcttcct ttttgaaaac gttaaaggct tataccggac tcttaaacat agagaatttt
      601 tcgaacaaat taagaatgaa ctagcttcag taggatatat cctgcatgaa agattgatta
      661 attcaattga atacggtgcg cctcaagatc gagaaaggat tattttagtt ggttttcttg
      721 ataagtcttt agccaaaaac ttcgaatgga gtggccaaat ataccccaat aaggaagctt
      781 tccagttccc ttggccaaca acaaacacat acgaagaaga ctcaagtatg gaatgcccgc
      841 aaggtattcc agaagaactt actatagaat actggttcga gaaaaacgat gttaccaatc
      901 atccaaacgc taaacatgta tttacggccc gagccggttt agcaagattc cagtcagtag
      961 aagaaggcga cgatagtaaa aaatcatata aaaggcttca tcgctggaga tactctccaa
     1021 cagcagcata tggtaataat gaagttcacc ttcacccata caaacctcgt cgtattacgg
     1081 tcgctgaagc attggcaatc cagtctttgc caaaaaattt tgagctacca ggggatatga
     1141 ctttgtcgaa tatgtttaaa acagtcggaa acggagttcc ctatatcgcc gcgctcggta
     1201 ttgcccaatc aattttgcaa catttaggat acagtaatga ggataacagc atcgaatctg
     1261 gttcaggcga tttcgagctt acccaaaaat acgctgttta actatataaa cgataggaat
     1321 agcgggaaga ttgagattgt acgtatccaa catcccgaag gtcctatcga aataaagaga
     1381 tttgatccta agaaaggtca aactcaagca acagctaaaa cagagagtat ttcgactcaa
     1441 atgctttggc gacttgcaaa tgcactcgaa gaaaacagac ctgtcaacgt tgaaagggtt
     1501 ttcggtgcca gttacaacac ccgctcggta ttggagagct tgctcgcaca tactccagag
     1561 ttctattggt gtaggccagc cagactggaa atcatgaatg cacaaaagtc tataaaacca
     1621 ggacataagc atctgatttt tctgccagat atgccacata ctaatggttt gttaattgag
     1681 caccagacta atattgttgt ctctgaaatg agcttcgatg ttgttcacca aagcgttgat
     1741 attgagacaa ttaaaccgac taaaggcatg actattgaag aaaaaagacg tcatgctcag
     1801 atacaaattg ccttagtaaa aataggttat tgtttaggtt tagatacttg ggtagcagcc
     1861 aatgatcgta gcttacaata taatggtaaa gctatagcgc aaatggacgg tgtaataagt
     1921 tcattatctg atgagcaagt actccaatcg tataatgatg cgattaaaga tgctcgtctt
     1981 atagactgca tttggtttag ggaaggccgc atgatgccag cagtaatgga gattgaacac
     2041 agtactggta taaaaagtgg tctgactcgc atgaaacaat tctatgatca tgcaccgaga
     2101 ttacaagata tccgttggac tatcgttgct cctgatgaat atcgagcaaa ggtgatagaa
     2161 tttgcaaaca tgccccaatt cattgaatta aaagctaaat tcttccctta ctcttcagtt
     2221 gaagaattat actctctatg cgaccgaaga aaacctaaag gaataaatga tttatttcta
     2281 gattctttta tggaagattg tgttatatag ctaagaacat caaattaaca tgaacctact
     2341 gatatatact atcagtaggt ttaaaatgat ttcaaatgtg ttccccaata tgagctattg
     2401 ccactctgtt tttatcac
//
LOCUS       DAZGHF010000290         2368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGHF010000290 DAZGHF010000000
VERSION     DAZGHF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.292"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1413..>2368
                     /gene="subA"
                     /locus_tag="ACHK3A_005069"
     CDS             1413..>2368
                     /gene="subA"
                     /locus_tag="ACHK3A_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO8743001.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWTELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSMNKKRDPVIRV
                     AALAQYRKGETPVLHGGGITGSRFGNGWVDIAAPGQNITFLRPDGKTGTGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEG"
BASE COUNT          687 a          498 c          524 g          659 t
ORIGIN      
        1 acaccggaat tgatccaagc taccgccatt atcttccgcg ctgacagaag ataataaaaa
       61 tgtgagatac ttcaatttta ttgaaaagct gaaaaaccct tacaatcctt gcagtttatg
      121 gagaaaattt tcacaaggta aattttaaaa caaacaaatt taatgtaata taattggggg
      181 aggattaacc atcgatgatt gccggaacaa aaaacaatat aaaccaacaa gatagaaaat
      241 catcacaaca aagggaaagg tctgtggata agagggggag gaactttctg cttcatgtca
      301 cataattgat atactttatc ccgcaacctc cagttgcatt atatataatt caacctcagg
      361 ctcccatcac aggccaaaaa taatatatcg caagccgcat caaatccgga tttttatata
      421 ataaataata cctgtttttt ccatcaccag tctgttaatc aacttaatat atttcagggt
      481 tattttacat tagcccggca aaagttatca ggcatgcagg atacattatc agggagagcg
      541 gtagtgaacg ccggtacctg cttccccaaa gttatttaat taacccgtat caacagggta
      601 acagcatggc aaaatccagc aattacgcac ctgttaaggt cattatttac gttgatatcg
      661 gtaaagatcc ctgccacact gttatcatta atcgttctga taattagttc gacaaaatat
      721 tagcaaacgt cgaaaacaag ctcaggaaac agatatccgg cctcaggaaa tatgggcata
      781 tactgctgat tgctgaccag cctgctacca tcgttgtatt acccgtccac attgcacgct
      841 cagaaggtgt ccttgtcaga tacctgtaag tggactgact gccccgtatt gatatctatt
      901 gaccagtcag ttcgactttc ccggggagca gctcgcatcc ccggtttaac ggtcattcat
      961 gaatatactc agtgacctga tatccggggc ttaccaatga accacaccac cattcactga
     1021 ttgccccggt aaaacggcgc tgtgatatcc tgttcgccat gatgcctgac ggaacttttt
     1081 attgtcataa acatgccagg caacttactt gcctcctctt attttttctg gttaagtttt
     1141 atccatcatg gctgtattta cttctgctct gaaggaggag gcagagcatc ttatcgctga
     1201 cctgttgcag aaatccagat gcatattacc gtgaaggtga gcgttaattc attggataca
     1261 taaatataaa aattaatgtt tttgagacgg gcttcactcg ctttattggt tgtgattgtt
     1321 tatcttttgt cgaaaacagg atatttcaga tgtgatgttc ctgtaagata ttgacctgca
     1381 atactgttta tttgtactga ctggaggata ttgtgcttaa gattttatgg ccgcatattc
     1441 tgttcctgct ttttatatct gtgtctgtca gggcagaaaa accctggtat tttgatgcaa
     1501 ttggcctgac agaaacaacc atgtcgctca cagacaagaa tacccctgtt gtcgtatctg
     1561 ttgttgattc cggagtagca tttgtaggag gcctcagcga cagcgaattt gccaaattca
     1621 gttttactca ggatggttcg ccattcccgg taaaagagtc tgaagcttta tatattcatg
     1681 gtactgccat ggcttcactc attgcctcac gtcatggggt ttatggtgtt tatcctcatg
     1741 cactgatatc cagcagaagg gttattcctg acggtgtaca ggactcatgg attagagcaa
     1801 ctgaaagcat tatgtcaaat gtttttcttg caccaggaga agagaaaatc attaatatat
     1861 cgggaggcca gaaggggata tcttccgcat cggtctggac cgaactgctt tcccgtatgg
     1921 gccggaataa tgagcgatta attgttgcgg cagtgggtaa tgatggcgca gatatacgaa
     1981 aactgagtgc tcagcagaga atatggcctg cggcttacca tccggtcagc agtatgaata
     2041 aaaagcggga tcctgtgata agagtggcag ctctggcaca gtaccggaaa ggagaaacac
     2101 ctgtattgca tggtggaggg atcaccggaa gtcggttcgg aaacggctgg gttgatattg
     2161 ctgcaccggg acagaatatt acattcctca gaccagatgg aaaaacgggg acaggtagtg
     2221 gaacctcgga agcaacagct atagtttccg gcgttctggc agcgatggtc tcatgtaatc
     2281 cccgtgcaac agcgacagaa ctgaagcgta cactgctgga gtctgcagac aaatacccct
     2341 cacttgcaga taaggtgacg gaagggag
//
LOCUS       DAZGHF010000291         2354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGHF010000291 DAZGHF010000000
VERSION     DAZGHF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.293"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            121..2262
                     /locus_tag="ACHK3A_005070"
     CDS             121..2262
                     /locus_tag="ACHK3A_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO8743002.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
BASE COUNT          650 a          561 c          556 g          587 t
ORIGIN      
        1 tgattgaatg attctcaatt acatatccga tggcgaaata acaaacggat agtgcttagt
       61 gagcaataag aaacaatttt cactcgcgac aatccgcgag tttttgggat aacagactaa
      121 atggctatgt tcacaccttc attctcagga ctcaaaggtc gggcgctctt ttcactgctt
      181 tttgcggcac cgatgattca tgcaacagac tctgtaacga ccaaagatgg cgaaacaatc
      241 actgttacag cagatgcaaa taccgcaact gaggcgaccg atggttatca acctctgagc
      301 acttccacgg cgacattaac cgatatgccg atgctggata tcccgcaggt ggtcaatacg
      361 gttagcgatc aggttctgga aaaccagaat gcgacaacgc tggatgaggc gctttataac
      421 gtcagtaacg tggtacagac caatacatta ggcgggactc aggatgcctt tgtacgccgt
      481 gggtttggcg caaaccggga tggctccatc atgaccaacg gtctgcgaac cgtacttcct
      541 cgcagtttca acgccgccac agaacgtgtg gaagtgctaa aaggcccggc ctccacgctg
      601 tatggcattc tcgatcctgg cggactgatt aacgtcgtga ccaagcgccc ggaaaaaaca
      661 ttccatggtt cggtttcagc cacctcctcc agttttggtg gcggcactgg gcaacttgat
      721 atcacaggtc ccattgaagg cactcagctg gcgtatcgcc ttaccgggga agtgcaggat
      781 gaagattact ggcgaaactt cggtaaagag cgcagtacat ttattgcccc gtcactcacc
      841 tggtttggtg ataatgcaac agtaaccatg ctctattccc atcgggacta taaaactccg
      901 ttcgatcgtg gaacgatttt cgaccttacg acgaaacagc ccgtaaacgt tgatcgaaaa
      961 atacgttttg acgaaccgtt taatattaca gatggtcagt ccgatctggc gcaactcaac
     1021 gcagaatatc atctcaatag ccagtggaca gcgcgctttg attacagcta cagccaggat
     1081 aaatacagcg ataatcaggc gcgtgttacc gcgtatgatg caacgacagg aacactgaca
     1141 cggcgtgttg atgcaactca gggatctacc cagcgtatgc atgctactcg tgcggatctg
     1201 caagggaatg ttgatattgc cggattctat aatgagattc tgggtggggt gtcatatgaa
     1261 tattatgatc ttctgcgtac agatatgatt cgctgtaaaa aagctaaaga tttcaatatc
     1321 tacaaccctg tttatggtaa taccagcaaa tgtacaacgg tttcggcgtc ggacagtgat
     1381 cagacgatca aacaggagag ctactcagcg tatgcacagg acgcactcta tctgaccgat
     1441 aactggattg ccgtcgccgg gatccgctat cagtattaca cgcagtatgc gggtaaaggc
     1501 cgtcctttta atgtcaatac tgacagccgc gatgaacaat ggactcccaa actggggttg
     1561 gtctacaaac tgacgccatc ggtatcctta tttgccaatt attcgcaaac atttatgccg
     1621 cagtcgtcaa ttgccagcta cattggcgat cttccaccgg aatcatctaa tgcttacgaa
     1681 gtcggggcaa aattcgagct attcgatggt atcaccgcag atattgcgct gtttgatatc
     1741 cataaacgta atgtgttgta taccgaaagt gttggtgatg aaaccattgc caaaacggca
     1801 ggccgtgttc gttcaagagg ggtagaagtc gaccttgcgg gagcattaac tgaaaacatt
     1861 aatatcattg ccagctacgg ctataccgat gctaaggttc tggaagatcc tgattatgca
     1921 ggtaaaccat tgccgaatgt tcctcgtcat accggttcgc tattcctgac ctatgacatt
     1981 cataacatgc ctggcaataa cacactgacg tttggcggtg gtggacatgg cgtaagccgt
     2041 cgttcggcaa ccaatggggc tgactattat ctgccaggct attttgttgc cgatgccttc
     2101 gccgcataca aaatgaaatt gcagtatccg gtcacactgc aattaaacgt caaaaacctg
     2161 tttgataaaa cgtattacac ctcttccatc gccacaaata atctggggaa tcagattggc
     2221 gatccgcgtg aagtgcaatt cacggtgaaa atggaatttt gatcaaaaaa gcccggtatg
     2281 tttaccgggc tgagactgat gacaaactcc aaattgtctg atgcgctacg cccatcagat
     2341 ctaccataga aatt
//
LOCUS       DAZGHF010000292         2293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGHF010000292 DAZGHF010000000
VERSION     DAZGHF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.294"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..227
                     /locus_tag="ACHK3A_005071"
     CDS             <1..227
                     /locus_tag="ACHK3A_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743003.1"
                     /translation="LLPAEMLIRCLEDQTGGRWIRAHEPVMLMKRLISNLKKARPECT
                     VPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            245..1489
                     /locus_tag="ACHK3A_005072"
     CDS             245..1489
                     /locus_tag="ACHK3A_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO8743004.1"
                     /translation="MTLPVDSLIAYTLEKMQERIVQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVS
                     RVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACM
                     SRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPGQMAARQRVTEGRMQ
                     PGSETEPGVETQSSVLKMLSSDSELSEKQGQKILSSDSEPSEKSEGYHRVRKPNHYVR
                     NITHSVIKNTYVLPEALTGQIREEEAIMLTSQLQALPEELAQGVLESLSGMLGSHSLQ
                     NPVGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGERGRATAQSVSSGKRTEPISAE
                     RLSGIVAGLRASLRRCSAMRSE"
     gene            1601..2080
                     /locus_tag="ACHK3A_005073"
     CDS             1601..2080
                     /locus_tag="ACHK3A_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743005.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWVTYSLYKARTGSTVGGGLQFMFLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
BASE COUNT          643 a          472 c          677 g          501 t
ORIGIN      
        1 ttcttttgcc agctgagatg ttaattcgct gtctggaaga tcaaacaggg ggacggtgga
       61 tccgtgcaca tgaaccagta atgctgatga aacgattaat cagtaacctg aaaaaagcca
      121 gaccagaatg taccgttcct gatgaagcgc tggcatatct ccggcgtaac tgtctggtga
      181 atatgggcga tatacccgtt accagcagac ggatgtctga cgtataacaa aaaggaaaag
      241 tgtaatgaca ttacccgtag acagcctgat tgcgtacacg ctggaaaaaa tgcaagagcg
      301 gatagtgcag aaaggccgac aacaggacgg aaaaatacaa agcggactgc tgtttaccgg
      361 aaatgtgcat gatgccattc cgcgtcgact gcttctggac acgcgacttt ctccgctgga
      421 taagatggcg tggatgatga ttcggctgta tgcacagaat aatgaaggtg cagttttccc
      481 cacatatgac gaactgcaac tgcagcttgc ttccccgggg aaaggaaaag cctccaggga
      541 gactgtcagc cgggtgctgc tgatgctgcg gatcacgggc tggctgagtc tgtgcaaacg
      601 ggtgcgtgat gagaaagggc gggtacgggg caatatttat gcccagcacg atgaaccggt
      661 gacgtgccgg gatgctgaaa tactggatcc acgttatctg gatatggtgg cagatgcctg
      721 tatgagccgg aaccggacca taagtcagac ggcccgggat attctgtgcg aaataaaaaa
      781 tgatccggga atgcgtcatt atcacagcca tatcagtcaa ctggaagccc ggatagggag
      841 tgcccagaca ccgggacaga tggcggcccg tcagcgggtg actgagggca gaatgcagcc
      901 gggttcggaa accgaaccgg gggtagaaac acagagttcg gtattgaaaa tgctgagttc
      961 ggattccgaa ctcagtgaaa aacaggggca aaaaatactg agttcggatt ccgaaccatc
     1021 agaaaaatca gaaggttatc acagagttcg gaaaccgaac cattacgtac gtaatatcac
     1081 acacagtgtg attaaaaaca cgtacgtatt gcctgaagcg ctgacagggc aaatcaggga
     1141 ggaggaggcc attatgctga ccagtcagtt gcaggcgctg ccggaagaac tggcgcaggg
     1201 ggttctggaa agtctgtcag ggatgctggg cagccactcg ctgcagaatc ctgtgggctg
     1261 gttgcttgca gtgctgaagc gagcccggga gggaaatttt tatccgccga agcagaaaca
     1321 acgtgttgtt gtgccagcct ccgtactggc aggagaaagg gggcgagcta ctgcgcaatc
     1381 agtttcatcg gggaaacgta ctgaaccgat ttcagcagaa cgtctgagtg gcattgtggc
     1441 tggactacgg gcctcgctgc gcagatgttc tgcgatgaga tctgagtgat tgcaatacgg
     1501 gctatgttcg cgggaacaaa aatcaaccag tgccgccagc gactggttaa aaagaaaaca
     1561 gaatcgggaa ctggtcaggt gaaacaaggt ggtaaatagc atgcagaaat acaaacagga
     1621 aagtctgagt gcagccatga aagaaagtgg cctgttgatc gctaaaattt gtgcctgcgt
     1681 gattgccgga gtggtggtta tctgggggct ggtgacaggc acagagaccc tgctcactgc
     1741 gctgaaaaca atgcacgaac agtacgataa cagtatgtgt tatatcgtat gtgccacagg
     1801 gtggtttttc ctggtgttaa gtaactggct gtgggtcaca tacagtcttt ataaagcgcg
     1861 tacaggcagt acagtcggag ggggcctgca atttatgttc ctcctgagtc tgctggcaga
     1921 atgcttttca ttttttgtga ttttctggtc attgtctcat ctggatataa ccgacattca
     1981 gttgctcgtg atggggataa cgacattaat gctgtttttg tctctgaacg ttaaggtagt
     2041 agtcatcatt aaccatgttg ccagtggcaa cagtgattaa aaaatgagcg gtgttgccag
     2101 tggcaacaga ggttaaaaaa tgagcagcgc tgccagtggt aacggtgatt aaaaaatgag
     2161 cggtgttgcc agtggcagca gagattaaaa aatgagcagt gctgccagtg gcagcagaga
     2221 ttaaaaaatg agcagcgctg ccagtggcag aagaggttaa aaagcgaaca gtgctgccag
     2281 tggcagaaga ggt
//
LOCUS       DAZGHF010000293         2153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGHF010000293 DAZGHF010000000
VERSION     DAZGHF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.295"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(72..560)
                     /locus_tag="ACHK3A_005074"
     CDS             complement(72..560)
                     /locus_tag="ACHK3A_005074"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8743006.1"
                     /translation="MFQLTPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AVDNDHHIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
     gene            complement(566..847)
                     /locus_tag="ACHK3A_005075"
     CDS             complement(566..847)
                     /locus_tag="ACHK3A_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO8743007.1"
                     /translation="MPSATQTARLEARISTELHAMLKRAAEIQGRTMTDFIIAAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
     gene            complement(1265..1840)
                     /gene="dolP"
                     /locus_tag="ACHK3A_005076"
     CDS             complement(1265..1840)
                     /gene="dolP"
                     /locus_tag="ACHK3A_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO8743008.1"
                     /translation="MRLLYSAVVLTSALLLQGCVAAAVVGTAAVGTKAATDPRSVGAQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPGRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADVASRVSGVKRVTTAFSYIR"
BASE COUNT          557 a          524 c          476 g          596 t
ORIGIN      
        1 tgttgcccgt taacatttaa cttcgtgggc tgacgttgcc acaagcaccg tatttccctg
       61 atttattctg tttattttat tgtggataat gatgccaatg gcatgaataa agttcttccg
      121 gactccggga acgccataaa gccataatgt ttgtaaaatg cgactgccgt gtcatctttt
      181 gcatcaacaa taagcgcata tgctgcaatt tcagatgtgt atgcacgata tagcgcatca
      241 gctaaaagtg ctcccccaag ccccgttcct tttgatgaca gacttactgc cagtcgtccc
      301 atacgaatag ccggaatagt gggatatcgc ggaagctttt ttctcaaatg ctcaggtaag
      361 tcagttaata atatactggc cgctgataac gtatagtatc cgatgatatg atgatcgtta
      421 tctacagcga caaaacaggc cgcgatgcgt cgtcgaatat cctgtgtgac atgctctttt
      481 atgtaacggt ccagagcatc agagccacat aaaaatgagg ttctgtcata tgtgctgtta
      541 aatggtgtca gttgaaacat tatatttatt ttgtgtacag catattatta cggcgtttaa
      601 aggcccgttt aagcgcatca ttcggctctg ttggtgacag taatgcctga gcaaaaattt
      661 cctgatctgt ccgggacagt cggattatat cggtttgttc gatagcgcgt tgggccgcat
      721 cctgaacggc agcaatgata aaatcggtca tagtgcgccc ctggatttct gcagcgcgct
      781 tcagcatggc atgtagttct gtgctgatcc ttgcttccag tcgggcagtt tgtgttgcag
      841 atggcatgat ttgtacctcc tgaatacact gtacggcaaa ttgccgtatt gtgcaaatta
      901 cactctgaca ccgggacggc agacagacat tctgcctgaa aagaaaaatc agattcagac
      961 aaaactatcc gggggatgtt aacgccacga tctttgacca ttgcatacag ataaaagctc
     1021 agattattta ccagcagtga catccggagt acaccgggat tttatctgtg agtgaacata
     1081 cccgggcaaa aaaatccccc gaattcaggg gggtgtcaaa gaggaattat gtatattggt
     1141 tattataatg agcacgcctc cggtattccg cgacgaatgt tcacgtggtg gcatgaacgg
     1201 cccggaaacg tgctcttctg atttttaacg taaatttaca ctaaaggcaa ctctgatact
     1261 gccttcaccg gatataggag aaggccgtcg tgacccgctt cactccgctc accctactgg
     1321 caacatctgc tgccgctcgc ccttcctgct ctgtaaccag gcccagtaaa aagacttcgc
     1381 tgttttctgt caccaccttg acactggatg atttgacacg ggcacttgtg agtaactgag
     1441 aacgaatttt tgtcgtaagc caggcatcac ctgccattat gaccggactg acgggtgtcc
     1501 cctgccggat ttcattatag acctcctgaa caccatcgac accgagtgct atctgacggg
     1561 cacgggcaga taattcacgg cccggagact gaccgatcag cagcacgcgt ccctgatatg
     1621 caatctcact gatgcgtgct tcttttctga tttgttcatc ttttgaaagt gcactgctga
     1681 ccctcagctc cagcacttca tcatccacct gcgctccgac actgcgtggg tcggttgctg
     1741 cctttgtccc gacagcagct gtacccacca cagcggcagc cacgcatccc tgtaacaata
     1801 aggctgaagt aaggacaaca gcagaatata ataaacgcat atctcccccg gaattgtttt
     1861 gatttttact tcatattccc ggaatttatc gtacaaaaag tttaccttca gtgaaacaaa
     1921 acaagctaca gactgttatt atcctccggc acagccggaa atagcggatg cgttacgtgg
     1981 catatctgta tctgtttttt gcacgaaaac tttgataccg tcgtaaagat tcttttttta
     2041 actatctggt tcgctgaaca ggatctcctt ttcgttatac acaggctaaa aggcaccgtg
     2101 gagcccccgg tgcctttttc ataaaacaga gaaaacacgt ctttgaatat acc
//
LOCUS       DAZGHF010000294         2152 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGHF010000294 DAZGHF010000000
VERSION     DAZGHF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.296"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            98..307
                     /locus_tag="ACHK3A_005077"
     CDS             98..307
                     /locus_tag="ACHK3A_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8743009.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            complement(327..560)
                     /locus_tag="ACHK3A_005078"
     CDS             complement(327..560)
                     /locus_tag="ACHK3A_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743010.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(649..1272)
                     /locus_tag="ACHK3A_005079"
     CDS             complement(649..1272)
                     /locus_tag="ACHK3A_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8743011.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYHL"
     gene            complement(1361..1870)
                     /locus_tag="ACHK3A_005080"
     CDS             complement(1361..1870)
                     /locus_tag="ACHK3A_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743012.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGINGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWPVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
BASE COUNT          585 a          584 c          465 g          518 t
ORIGIN      
        1 taaaaacaga aaattaacaa cgcacatcag gaaacgtcgt ctcctgattc agtgatacca
       61 catgaaaatt tagtgaacgg ttaccgttct gaagtctatg tcagaagata ttccattacc
      121 aaaaatcaat cagcgataca gagacgatca tggcgcgctt gtcaccgtga ccagtgttga
      181 ggaaacccgg gtggtgttta tgcgggacgg ctatccgcat ccctgcatgc ggccgatgta
      241 caacttcctg ggaaaattca aacccgaacc ccggaaagaa ccccctgccg gcaatcacac
      301 tgcctgatgg cagcataatc cacttattaa ttctcatccc cggagacaag cccagaaaac
      361 cactccgggt gttcactgta tgccgtgttc agcgcatccc aagaaacgtt gatataaaag
      421 ctgtcctgca atttgcgggt aacaatttct gccacccggt tacggttatc cgcctgtccg
      481 gcataacctc tctctgccag ccactcattc agttcatgtt ccggagacag attgataata
      541 tcgtaatcgg accttgccat tcccgtttcc ttagcacatg ttttcaggct cggggataaa
      601 gaatgtcgta ctgctgctgc cagagcaata ttccccctga tggtcgaatc agagatggta
      661 ggccggtact ttgcgggttg ttcccgtcag attatcatgc acctccgcgg tcgttgtcac
      721 caccggcatt ccgctctttc tcagcctcag gacatcagcc gcaatacgct gactaccgaa
      781 ccagaacagg ccgtccagtg cagtcaccag caaccccgcc tccagcgcat gcttcagccg
      841 ttcacggggc gctttcactt cccgggcaat ctgctggtat ggcgatgatg tgttttcatt
      901 cccaatcacc cggcgaatac gatgagacag atgataccgg tatgtatccg gtacaccgga
      961 aaggctggcc ttcaggctgt acacgcagcc aaatcgttta tcattgaaca ccacattttt
     1021 ctggctgatg ccccattctt cacgcagcgc ggcaatcagt tgtggtgtcc gggtaagcaa
     1081 caagcgaaaa ggcagttcaa aactggtgac gtaatccaca ttcagcagtg caatacgcag
     1141 ccgttcttcc ggcccggcct ctgtctgccg gcactcctcc agaacatcct gccactgctg
     1201 gtgaaggcgt ggaagttcat tcagttcagt aaaactgtat ttatccgcaa gatagtcaat
     1261 tcgtgtacgc atactgaaga gtattccgta taaagattca gctggcaaaa ctttatcagt
     1321 ctgtaaaaac taacggaaga gtcgatattt ctcccgtcca tcaccggatg attgttgcaa
     1381 taccccgtgg catcagagac tgaacagccg tttttaacgc cacgtattgc cctgatgaac
     1441 caggccgggc aacccgaaaa catctttcca cccggcattg tccgccagca cttatcaccg
     1501 gccaggtctg ttgcagtaaa tccaccagtt gcgcacattc ttcatcagcc atgacgccga
     1561 ctcgcggatg gcaaatactc gtctggctga gcagcaacag acatcgtatc tgtgctcctt
     1621 catgttgttc ccgcaaccag cgtaatacca ccggcgctga tgggctgacc gtgtgattat
     1681 gctccgtaat acgttcgtgt acccgtcggt gaaaggaact acaaaatgtc tgaatctgtt
     1741 gaccattgat tccttctgcc gaatagagcg tgaagtgaaa tgccaacaga tttggccagt
     1801 ggtccaccgc ctgctgaaca aaacgccgga tttcccccgg ctctgaaatt aaggcttcgg
     1861 ttatttgcac gaaatgttcc ttttcaaccc ctgttgaatt ttgtcaagcc ggtgcaggca
     1921 cagtaaagcg cgcatcaaca cctgaaaggt acgatacaaa cacaagttac aaacaataag
     1981 aacggaagga caattaatca gtgaggaata ataactaaat taaccaaaag aatgttactg
     2041 acaatactac caatacttaa atagcaatat attaattaat gtaagaacag atgcccggta
     2101 agacatatac acaatacatc cacaaacaca ctcttttcat taaatcaatc ac
//
LOCUS       DAZGHF010000295         2127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGHF010000295 DAZGHF010000000
VERSION     DAZGHF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.297"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..535
                     /locus_tag="ACHK3A_005081"
     CDS             <1..535
                     /locus_tag="ACHK3A_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO8743013.1"
                     /translation="PGGLPWLSEADRRLQIKSALPWWLVCRGEIHRFRCVPHLTGRRF
                     EHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLAENGFYRVSPSCAQA
                     GDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRRTHSVWRHRHWSA
                     SAFTGIYNDLVAASACM"
     gene            532..1113
                     /locus_tag="ACHK3A_005082"
     CDS             532..1113
                     /locus_tag="ACHK3A_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8743014.1"
                     /translation="MNTAAEAIRALSLQVPGFRGQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEQLGEGAVIHIVPRLAGAGGNGVFQVVLGVAAIVGSFFTGGATMALWGAALSAGGL
                     TATTMLFSLGVSMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
     gene            1535..>2127
                     /locus_tag="ACHK3A_005083"
     CDS             1535..>2127
                     /locus_tag="ACHK3A_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743015.1"
                     /translation="MGKGGGKGHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLERNGHWVTEKDVTINGKTT
                     SQYLTSVILNNLPERPFNIRMVRETADSTTDQLQNRTL"
BASE COUNT          493 a          531 c          668 g          435 t
ORIGIN      
        1 tcccggtggt ctgccctggc tgagcgaggc ggaccggcgg ctgcagataa agagtgccct
       61 gccctggtgg ctggtctgcc ggggtgaaat tcaccggttc cgctgtgtgc cgcacctgac
      121 cggacggcgc tttgaacacg gtgtgacgga ctgttacacc ctgttccggg atgcatacca
      181 tctggcgggg ataacgctgc cggattttgt gcgtgaggat gactggtggc gcaacggtca
      241 gaacctgtac ctggacaacc tggcggaaaa cggcttttac cgggtgtctc cgtcctgtgc
      301 acaggcgggc gatatcctgc tgtgctgctt cggcgcatcg gtgccgaatc atgccgccat
      361 ttactgtggc aacggtgaac tgcttcacca tatacctgaa caactgagta aacgggagag
      421 gtattctgaa aaatggcaac gacgaacgca ttctgtctgg cgtcaccgcc actggtccgc
      481 atctgccttc acggggattt acaacgattt ggtcgccgca tcagcctgta tgtgaacacg
      541 gcagcggagg ccatccgtgc cctgtcgctg caggtgccgg gattccgcgg tcagatgaac
      601 gaaggctggt accagatacg tattgccggt gaggataccg caccggaggc agtgtacgcc
      661 cgtcttcacg aacaactggg tgagggagcg gtcatccaca ttgtaccgcg actggccgga
      721 gccgggggaa atggtgtttt tcaggtggtg ctgggggtag cagccatcgt gggctctttc
      781 ttcaccggcg gcgcaacgat ggcgttgtgg ggcgcagccc tgagtgccgg agggctgact
      841 gccaccacga tgctgttctc actgggtgtc agcatgatac tgggtggtgt ggcccagatg
      901 ctggccccga aggcaaagac gccggagtac aaaagtacgg ataacggtaa acagaacacg
      961 tatttttcgt cactggacaa catgattgcc cagggtaacc ctatgccggt gccttatggt
     1021 gaaatgctgg ttggttcacg acggatatcc caggacatca gcacccgtga tgagggcgga
     1081 gacgggaaag tggtggttat cgggcgggga taaaaataaa aaaaatcccg cagagttagc
     1141 ggagctgcgg gaaataacga tgaagattaa cgttatggag ttattttttt gggcagaaga
     1201 acagtaacgc agcgtcatta ttgcggctac aggcaattgc cggaagtgtg aagaatttca
     1261 gaaattttct gttgcggagc tgaaataaaa aacgctcccg caatacacct ggatattaac
     1321 gggaggaacg aatagtcacc ttaaggagtt gcgattaatt gcttttattg accgcaggta
     1381 atctacccat ccggcaaggt ttcctccacg ggaatttgcc ggaaatgtga agaatttcag
     1441 aaattttatt ccgtcatgac acaggcaccc tccggggtgc ctgtcgtttt ctggcataaa
     1501 cagattcaga catcagacag gagaggggga cagagtgggt aaagggggcg gcaaggggca
     1561 cacaccgcgt gaggcgaagg acaatctcaa atccacgcag atgatgagcg tgattgatgc
     1621 cattggtgag ggaccggtgg aaggtccggt gaagggactg cagagtattc tggtgaacaa
     1681 aaccccgctg acggacacgg acggtaatcc cgtgatacac ggtgtgaccg ccgtctggcg
     1741 tgccggggag caggagcaga caccgccgga aggctttgag tcctccgggg cagaaaccgc
     1801 actgggcgtg gaggtgacga aggcaaagcc ggtgacgcgc accatcacgt cagcgaacat
     1861 tgaccgtctg cgggtcacct tcggggtgca gtcactggtg gagaccacct caaagggtga
     1921 ccgtaatccc tcttctgtcc gcctgctgat tcagcttgag cgtaacgggc actgggtgac
     1981 ggagaaggat gtcaccatta acggcaagac cacctcgcag tacctgacgt cggtgattct
     2041 gaataatctc cctgagcgcc cctttaacat ccggatggtc agggagacgg cggacagcac
     2101 cacggaccag ctgcagaaca gaacgct
//
LOCUS       DAZGHF010000296         2124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGHF010000296 DAZGHF010000000
VERSION     DAZGHF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.298"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            175..456
                     /locus_tag="ACHK3A_005084"
     CDS             175..456
                     /locus_tag="ACHK3A_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743016.1"
                     /translation="MNTTCARLPSADTLAEQAAKTASGWMSDAVIAIDELFGKGYAKQ
                     HPELVAAFMQTAGLDEAAMHLRGLVLANQRIDDTLEVLSEHIMLLNEPQ"
     gene            525..947
                     /locus_tag="ACHK3A_005085"
     CDS             525..947
                     /locus_tag="ACHK3A_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO8743017.1"
                     /translation="MRRDMQLVLERWGRWAASEEYCSMVDWPSMSVTSRDVTDSGKPG
                     CSDEDGAVIDTCIAHMSMVCPYDDLLILGLRFIGGLSTRAIAEAIDRSHLSVRTSLKA
                     SEAFLEGALTLQGVRLDMDPEVVLPERVVCAQKNMLWL"
     gene            1033..1128
                     /locus_tag="ACHK3A_005086"
                     /pseudo
     CDS             1033..1128
                     /locus_tag="ACHK3A_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            1117..1193
                     /locus_tag="ACHK3A_005087"
     tRNA            1117..1193
                     /locus_tag="ACHK3A_005087"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1151..1153,aa:Arg,seq:tct)
     gene            1754..>2124
                     /locus_tag="ACHK3A_005088"
     CDS             1754..>2124
                     /locus_tag="ACHK3A_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO8743018.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPTGGEGAVISATSDPEYYFVVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPG"
BASE COUNT          560 a          461 c          569 g          534 t
ORIGIN      
        1 atatacgcgt gcgcgcacgc atgtatgtaa gaattttatt tttccaaaag gttgagtagg
       61 ttgagtaaga gtatgatttt tatgtttttt aggttactca accttttgcc cgtaaaaaaa
      121 ggttgagtaa agaaaacttg cagaaaatca caaataacca aacggagtaa aaacatgaat
      181 acaacctgtg caagattacc aagtgcggat acgttagcgg aacaggcagc aaaaacggca
      241 agtgggtgga tgtcagacgc cgtaatagcg atagatgagc tttttggaaa gggctatgcg
      301 aagcaacacc ctgaattagt tgctgccttt atgcaaacag cgggcttaga tgaagctgca
      361 atgcacttac ggggacttgt gttagctaac caacggattg atgacacact ggaagtgctt
      421 tcggagcata ttatgctgct taacgagccc caataaaccc gatgcggata aacagacgta
      481 gttctcagaa aattttttcg tagcaaaacg tagaggccag cgttatgcgt agagatatgc
      541 aacttgttct ggagcggtgg gggcgatggg cggcgagcga ggaatactgt tcaatggttg
      601 actggccctc aatgtcggtt acttcccggg acgtaaccga tagtggcaaa cccggttgct
      661 ctgacgagga cggagcggtg attgatacct gcatcgcgca tatgagtatg gtttgcccgt
      721 acgatgattt gctgatcctg gggttacgtt ttataggcgg cctctcaacg cgtgcaattg
      781 ctgaggcgat agaccgtagt catcttagcg tacggacatc cctgaaggcg tctgaagcat
      841 ttctggaagg agctctgact ctgcagggag taaggttgga tatggatcct gaagttgtac
      901 tacctgaaag ggttgtgtgc gcacaaaaaa atatgttatg gttatagcgt ctataattgt
      961 atgcttactg ctgaaaggtt ccgataacac cggaaccttt tttcttgtct tctgctggga
     1021 aaaattaata aaacaggaaa gaaaaacatg acatcgcact ggttcagtgt cagccagtgg
     1081 cagttaccaa acgaggatga ttacagaaaa cttcatgcgt tgttagctca gccggacaga
     1141 gcaattgcct tctaagcaat cggtcactgg ttcgaatcca gtacaacgcg ccacattgat
     1201 ttttctgtct cgcttcggtg ggcctttttg tcgctgaaaa agaaaacaac gggtggttag
     1261 cagagtatca gttaatcatt gaaattttta aatgcctcac aatttcgtaa gttgatgatt
     1321 gtctggctga tggcgaattt gttaaaaatc gcatcgcatg gcgaatcccc ctgtgcggag
     1381 gggcgactgg tgaaaaatcc ttacttgtga ttcattatcg acgcgggttc ggtggcaccg
     1441 ggccgaactc accgggaggc acccggcacc atgtacatca tgatacagat acgcagcgtc
     1501 agcccctctc cggaggggct tttttatgga taaaaaaagc ccgcgccagg agacgcgggc
     1561 ggcaaggaat aaacaacaaa acgtgaagta atatttcagc tggcgaataa tacccgacag
     1621 taatcactct gcgcaactgc gcggcctttt tcgtattgcg ggctgtagtc ttccttctgt
     1681 cattgtcctt taacttccgg acctcagccc gctctttatc tgattcagta cactatcccg
     1741 gccgggagga ttcatgacat ttaaacacta cgatgtggtc agggcggcgt cgccgtcaga
     1801 ccttgctgat gcacttgctc aaaaaattcg tgaaggatgg caaccatacg gcgggccgtt
     1861 ttcttcgtat acggatgatg gcgcagcact tattcaggcg attgtcgcag aaggtgatgt
     1921 gagcacacct gttgtggtga agccgacagg tggagaaggt gcagtaatca gcgccaccag
     1981 cgaccccgag tattactttg ttgtggttct ggcggggcag tcaaacagca tggcatatgg
     2041 tgaaggcctt ccgctgccgg agacatatga ccgtccggac ccgcgtatta agcagctggc
     2101 gcgccgcagt acggtgacac cggg
//
LOCUS       DAZGHF010000297         2120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGHF010000297 DAZGHF010000000
VERSION     DAZGHF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.299"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..523)
                     /locus_tag="ACHK3A_005089"
     CDS             complement(<1..523)
                     /locus_tag="ACHK3A_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO8743019.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENAWHLMEDHRGKAVWRLESGSALLITEPGPLPENVTEIKP
                     DGEFCRWDGSSWVKDEAAEQAYRVSEAENKKRELIQEATNTINILQDAVDLNMATDDE
                     SALLPLWKKYRVLL"
     gene            complement(523..>2120)
                     /locus_tag="ACHK3A_005090"
     CDS             complement(523..>2120)
                     /locus_tag="ACHK3A_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO8743020.1"
                     /translation="MDERQRRENVDIYGKATFFDDGWLVKKGETWTVNKGVGYVEGLR
                     AELSTHHPMTTPAFPSRIWLNVWWTGTLTSEWATEHEIITADPDVTEERLRKTYPRDG
                     IQQYFCAIAEIDEDGNVTDLRPRGTPEERRSLDALKKHEKSRNHPDATLKEKGFTQLS
                     SATTSTSETQAATPKAIKIAMDNADGRLAKNRNGEDIPNKELFLINVGAPRVYAKDGY
                     VGETGQRWTTEQFVEWLNERGAFRPGLWIFKANISLSRHSVLTDTGYGDICLGGCLIE
                     VSGDINGGIIRITTGLESPNPADMYLRTQFTGVVLNKGKSTIWYRTYTDKNKPTPQDI
                     GALPASELLPAGVPLPWPSDTPPAGYAIMVGQTFDKARYPLLAKAYPDGRIPDMRGWT
                     IKGKPASGRAVLSQEQDGIKSHTHGGSVSNTDLGTKTTSSFDYGTKTTSTNGQHEHSV
                     PLRRQDGGGINFDWLDGSSSGSHIGNGYVPAAGNHQHTIPIGAHTHTLALGSHGHGLT
                     INPTGNAENTVKNIAFNYIVRLA"
BASE COUNT          379 a          555 c          581 g          605 t
ORIGIN      
        1 tcagtaatac ccggtatttt ttccacaggg gaagcagggc gctttcatcg tcggtcgcca
       61 tatttaaatc cacggcatcc tggaggatgt ttattgtatt tgttgcttcc tgtattaatt
      121 cccgtttttt gttctccgcc tcgctcaccc ggtaagcttg ttctgctgct tcatccttaa
      181 cccatgatga accatcccac cggcagaact ccccgtccgg ttttatttct gtcacgtttt
      241 ccggtaacgg gcccggctca gtaatcagta aggcgctccc cgactccaga cgccagacgg
      301 ctttaccccg gtgatcttcc atgaggtgcc aggcgttttc atcctcatca aagatagcca
      361 cgtgtccttc aggaatgtca ggcggggcaa tgtccgttga gtgcgcaggc agtccggtat
      421 gcggagggat ccaggcatcg ccttcgccga taaattcatt tgttccggct gacaggttgt
      481 agattttaat tgtctgtggg tgttcgctca ttttaaaggt cattatgcaa gcctcacgat
      541 gtagttaaat gcgatgtttt tgacggtgtt ttccgcatta ccggtggggt taatggtcag
      601 gccgtgacca tgtgaaccca gtgcgagcgt gtgggtgtgt gcgccaatgg gtatggtatg
      661 ttggtggtta cctgcggcag gaacgtatcc attaccaata tgagaaccag acgatgaacc
      721 atccagccag tcaaagttta tgcctccgcc atcttgtctt ctcaggggga cgctgtgttc
      781 atgttgtccg ttcgtagagg ttgttttggt tccgtaatca aatgatgagg tcgttttcgt
      841 gccaaggtca gtatttgaga cactcccccc gtgggtgtgg gatttgatgc cgtcctgttc
      901 ctgtgacaat accgcacgac cgctggcagg ttttcccttg attgtccagc cacgcatatc
      961 cgggatgcga ccgtccggat aggcttttgc cagcagggga tatcgtgctt tatcgaatgt
     1021 ctgccccacc atgatggcgt aaccggcagg tggggtgtct gacggccacg gcaggggaac
     1081 gcctgcgggt aacaactcac ttgcaggcag tgcgccgatg tcctgtggtg tgggtttgtt
     1141 tttgtccgtg taagtgcggt accagatggt gcttttccct ttatttaaaa caaccccggt
     1201 gaactgtgtg cggaggtaca tgtcggcagg gtttgggctt tcgagacctg tcgtgatcct
     1261 gatgatcccc ccgttgatgt cgcctgatac ttcgataagg cagcctccaa ggcagatatc
     1321 accatagccc gtatcagtga gcacgctgtg ccgggaaagt gaaatattgg ctttgaatat
     1381 ccagagtcct ggtctgaagg cgccacgttc attcagccat tccacgaatt gttctgttgt
     1441 ccagcgttgc cctgtttcac ccacgtaccc gtcttttgca taaacgcgtg gtgcgcccac
     1501 gttgatcagg aacagctcct tgttggggat gtcctcacca ttgcggtttt tcgcaagccg
     1561 accatcggcg ttgtccatgg cgatctttat agcctttggc gtggctgcct gtgtctctga
     1621 tgtactggtg gtggcgctgc ttaactgtgt gaagcccttt tctttcagcg ttgcgtccgg
     1681 gtggttgcgt gatttctcgt gttttttcag ggcgtccaga ctgcggcgct cttccggggt
     1741 tcctctgggg cggaggtcag tcacgttgcc gtcttcatca atttcagcga tggcacaaaa
     1801 atattgctga atgccgtccc tcggatacgt tttccgtaaa cgctcttccg taacgtccgg
     1861 gtcggctgtt atgatttcgt gctctgtcgc ccattcactg gtgagcgtgc cggtccacca
     1921 cacattcagc cagatacggg acgggaaggc gggcgtggtc atggggtggt gagtgctcag
     1981 ctctgcgcgt agcccctcca cgtaacccac ccctttgttg actgtccagg tttcgccttt
     2041 cttaaccagc cacccgtcat caaaaaaagt tgccttcccg tagatatcga cgttttcgcg
     2101 gcgctggcgt tcgtccatgg
//
LOCUS       DAZGHF010000298         2075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGHF010000298 DAZGHF010000000
VERSION     DAZGHF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.300"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..226)
                     /locus_tag="ACHK3A_005091"
     CDS             complement(<1..226)
                     /locus_tag="ACHK3A_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020236720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO8743021.1"
                     /translation="MRDLKDKIYFYTEGEFLEMLEEIVNATSKDKSLKGKKLEKYLDT
                     LVDHFIKITEHPKKGDLIFYPNSQEDGEPEN"
     gene            complement(232..2013)
                     /gene="tssD"
                     /locus_tag="ACHK3A_005092"
     CDS             complement(232..2013)
                     /gene="tssD"
                     /locus_tag="ACHK3A_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO8743022.1"
                     /translation="MSDIVYLRIIGEKQGYISSGCGTYASVGNCWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECITVDYDYILCKHLIVNTEFDWLAFPAGYNSLFI
                     PPKSPPASNLKPEPLPVVNLPLSSPAVNPVYAKSCLKEKGCTDAGTAEEPAENFGQVA
                     IFAQPVVDDCCGYHHPEANDVGQPAEAQTMLLFPGSVLAAQIWGKWSLSGILSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLDQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDLRKLPGVVTGRGVPLSLGTRWLDMSVSNNGNGAPIPAHIADKLRGREFKTFDEF
                     REALWLEVSKDSVLLAQFIKSNQNNVSQGYSPYVPEEGYYYGPNEIVKKFQIHHVVAI
                     EHGGGVYDIDNFRIVTPRLHDEIHYRR"
BASE COUNT          549 a          501 c          441 g          584 t
ORIGIN      
        1 tgttttcagg ttcaccatct tcttgggaat taggatagaa aatgagatct ccctttttag
       61 gatgttcagt aattttaatg aaatgatcca ctaaggtatc gagatatttt tctaattttt
      121 tcccttttag ggatttatct ttgctagttg catttactat ctcctcaagc atttcaagaa
      181 attcaccttc tgtgtaaaaa tatattttat cttttaaatc acgcatatac attatctcct
      241 gtagtgaatt tcatcatgta gtcggggcgt aacaatccta aaattatcaa tatcatacac
      301 tccaccacca tgttctatag caactacatg atgtatctgg aatttcttaa ctatctcatt
      361 tggtccgtaa taatacccct cctcagggac atagggggaa tatccttgac taacgttatt
      421 ctgattactt tttataaact gcgccaataa cactgagtct ttactcacct ccagccacag
      481 cgcctcgcga aactcatcaa aggttttaaa ctcccgtccg cgcaatttat cagcaatatg
      541 cgccgggatt ggtgcgccgt tgccgttatt ggataccgac atatccagcc agcgagttcc
      601 tagtgaaagc gggacgccgc gccctgttac aacaccaggc agctttcgca gatcctcctt
      661 caggtaaaca tagatgggct taataccgga tcccgccgga aaaaccagaa tataatcgtt
      721 gaattctttc ggatcaggaa ccgggaatgg cggcgtggta taggtatcgg tgccgtccgg
      781 aatcggcgtc accagaatgg tgggctgatc cagcggcggc ctgtcgttac cggtatggcc
      841 cggaacattc ccatgttccg gattttccgg cgtccaggta atcagggggc catcgacatc
      901 gtgagcgggg gtaaattcat aacgctgcgt ctgctcgttc cactgcatgt tcgccacgcg
      961 gacgttttca tacggcgtac cttcacccgt atgaacacca tacacctgag gcttgccgtg
     1021 aatgtcagtt ccccagaaaa aacgtacccg ggtggtggcg gtactgcctg ctagtgcttt
     1081 ctgccgcagt tcttcctcat accagaactc atcacgtccg ggcacgcgag cactgccctc
     1141 gcccgcagag ggaatataca aagcagatgc caacgcaccg atgtaagagc cgcgggttgc
     1201 actgagtatg ccactgagcg accattttcc ccatatttga gccgccaaca cactacccgg
     1261 aaacagtagc atggtttgag cttctgcggg ttgcccgaca tcgttagcct cgggatggtg
     1321 gtatccacag cagtcatcaa ccactggctg agcaaaaatc gctacttgac cgaagttttc
     1381 agcgggttct tctgccgttc cagcatctgt acatcccttc tccttcagac aggattttgc
     1441 atagaccggg ttaaccgctg gtgaagagag tggaaggtta acaactggta gaggttcagg
     1501 cttaagatta ctggcaggtg ggcttttagg cggaataaat aagctattat agccagcagg
     1561 aaaggccaac cagtcaaatt ccgtattgac tattagatgt ttacataata tgtagtcata
     1621 atcgacagtt atacattcgg taggaagatt attgtcagaa atgtttacat gaatagcagt
     1681 taatgaagca tttcttagct gaatataata ataccgttcc cagcgcccgc ttttatttat
     1741 acgatacaaa tcgatttcaa taaataaccg ctcattctga ttgatggcat tagacagtag
     1801 cggtgagctt ttatcaatga gtttgcaaaa ttgtagcccc tgtagattaa cgcctttacc
     1861 agtactggta atggcgttgg tgagtgcaaa ggcaaaaatt tcatcttcat ggccaacctg
     1921 ccagcaatta ccaaccgacg catacgtccc acagcctgaa gaaatatagc cctgcttctc
     1981 accgattatt ctcaggtaaa caatatcgct cactttattt cattccagtt tatccttaac
     2041 ttatttgcat taaatagaaa acttaattct cgatc
//
LOCUS       DAZGHF010000299         2072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGHF010000299 DAZGHF010000000
VERSION     DAZGHF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.301"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1744
                     /locus_tag="ACHK3A_005093"
     CDS             <1..1744
                     /locus_tag="ACHK3A_005093"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO8743023.1"
                     /translation="TDADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNI
                     LPGEQTALLSEAGKGRARADWLTGMNLSRLYTLKARERGISGVLSVGRVQTPTLAMVV
                     RRDQEIATFVPVSWWQVIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLC
                     RQQSSAVVQDVVQKREKTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKAT
                     TYPRTDCGYLPASMHEESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHA
                     IIPTRQVFDLSSLNGDELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRV
                     NVIIGWKQLFPPENHADEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFT
                     EGCLITAMKNASSGVTDPALKKILRENAGLGTEATRAGILETLFARNYIERAGKHIRS
                     TQLGRELVAALPDSLTNPGMTALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRN
                     QTVNLSVESGPSCPLCNGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINF
                     SHKQKSGDVGDKLN"
BASE COUNT          494 a          462 c          630 g          486 t
ORIGIN      
        1 cacagatgcc gatcgtgaag gagaagtgat agccagagag ttgctggagt actgtcagta
       61 cagagggcgt gtcaggcgtc tgtggttatc tgcactggat gaggccagta tcaggaaggc
      121 gctgaataat attctcccgg gcgaacagac tgcgcttctc agtgaagccg gtaaggggcg
      181 tgcccgggct gactggctga cagggatgaa tctgagccgg ttgtataccc tgaaggcacg
      241 ggaacgtgga atttctgggg ttttgtcagt gggccgggtg cagacaccca cgctggcaat
      301 ggtggtcaga cgggatcagg agatcgccac atttgtgccg gtttcctggt ggcaggtgat
      361 tgtctggctg gaaaaggagg ggctccgttt ccgcagtaac tgggtggcag ctgagcagta
      421 ctgcgatgat gagggacgtt gtgtgaatgt gcaggcggcc ggtgctgttc tcggtctgtg
      481 ccgtcagcag tcctctgctg ttgtacagga tgttgtacag aagagggaga aaacaccgcc
      541 acctttgtgt ttttcgctgg gaacgttgca ggaagcctgt tcccggaagt ttggcatggg
      601 ggctcagacc gttctggata tcgcacagtc cctttatgag actcacaaag caacaactta
      661 tcccaggaca gactgtggtt atctgcctgc ctcgatgcat gaggagagtt ctgaagtgct
      721 tgaggctgtc gggcgttcag atccgtccgt gcttccacaa ctggctgaac tggaatcagg
      781 acgggtatcc cgtatctgga atgataaaaa aattacggct caccatgcca ttattccgac
      841 ccggcaggtt tttgacctca gctcactgaa cggtgatgaa ctgaaggtat atcaactgat
      901 tcgtcagcac tatctggcac agtttctgcc ggagcaggaa gctgatgtta ctgaggcaac
      961 atttgtgatt ggggggcagt tattccgttc ccgcggaaga gttaatgtga ttatcgggtg
     1021 gaaacagtta tttccaccgg aaaatcatgc agatgaagaa aagcctgaca gtgatgaaag
     1081 tggaaacata ttgccccctt tgataaaggg agaactctgt gctctggatg gtgctgaaca
     1141 gaaggagctg aaaaccgtac ctccccgtcc ttttactgaa ggctgtctta ttactgcgat
     1201 gaagaatgct tcgtctggtg taacggatcc ggcgctgaaa aagatcctga gggagaatgc
     1261 gggactggga acggaagcca cacgcgcggg gatcctggaa acgttatttg cgcgcaacta
     1321 tattgagaga gcgggaaaac atatccgttc cacgcaactg ggaagagaac tggtggcggc
     1381 attgccggac tctctgacaa atccgggtat gacggcttta tgggaacagg cgctggatga
     1441 tgttgcccgg gggaaaatct cactggaggc gtttatggag aaacagcatc agtggatcag
     1501 gcacctggtg ttatccggac gtaaccagac ggttaatctg tcagtggaat caggtccttc
     1561 atgtccactc tgtaacggtc cgacggtgaa acggaaggga aaaagcggtt ttttctgggg
     1621 atgctcccgt tatcccgact gcaggggaat tgctggtgag cagggaaaca gcaggggcag
     1681 gaaaagaaaa attaattttt ctcataaaca aaaatctggt gatgtcggag acaaactgaa
     1741 ttaaaattgc aggcgagttc tggtatcacc ctgttgacca cagtggggct gacagccctg
     1801 agaggtccgg taacccgtta tgcacacggc ggggttacaa ggcacatgca ctccgtgaaa
     1861 accgtgccgg gagtcgctct ctccggagaa gatgagaatc aacaccggga tttggtatcc
     1921 ccctgaagca ccggcttgcc ggtgcttttt tgtttttggt tagctgttga ctttttccat
     1981 cagtgatttc agtctgtccg ggcacataac gtcgctccgg cgaccaccaa tgctccacaa
     2041 gtggcctgag aggagcgcct tcgggtggct ac
//
LOCUS       DAZGHF010000300         2069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGHF010000300 DAZGHF010000000
VERSION     DAZGHF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.302"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            180..647
                     /locus_tag="ACHK3A_005094"
     CDS             180..647
                     /locus_tag="ACHK3A_005094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743024.1"
                     /translation="MKGKIIPEKNKIKNLINQERDAIALWIKDGMRLPDIYRLLKEKH
                     PELTFSQNGFVFSLRRDASDLHENALFNRSTVAVFMQKQHSEMTLMVNSGCLLKNVHE
                     ALFSHISYSVFLRYIVKLYPDLHYQAKLNRKGAVCGELHHEENASVYQNDEVC"
     gene            complement(770..1051)
                     /locus_tag="ACHK3A_005095"
     CDS             complement(770..1051)
                     /locus_tag="ACHK3A_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743025.1"
                     /translation="MKTNKKGTTWHIFYRENSGAEVLLEIPSFRECLSVSKELMTPSN
                     YMICIEKNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
BASE COUNT          563 a          368 c          438 g          700 t
ORIGIN      
        1 tcatggcagc ctcatgtatt tatgtggaga ttatttcatc ggatattatt ttatccagga
       61 atgttgtttt gttaaagtga gtaagtctta tagattatgt ttctttactg aattccgggt
      121 atgtagacaa agctggttct gtatatgtga acttctttgt tgttcctgag ggagataata
      181 tgaaaggcaa gattatacca gaaaaaaata agataaaaaa cttaatcaac caggagcgtg
      241 acgccattgc actctggatt aaggatggaa tgagactgcc agatatttac agattactga
      301 aggaaaaaca cccggaactg acgttttcac agaatggttt tgtattttca ctgagacgtg
      361 atgcaagtga tttacatgaa aatgcgcttt ttaatcgcag tactgttgct gtatttatgc
      421 agaaacaaca ttctgagatg actctcatgg ttaattctgg ttgtttgctg aaaaatgtgc
      481 atgaggcgtt attcagtcat atatcttact ccgtgtttct tcgatatatt gtgaagttat
      541 atccagattt acactatcag gcaaaattaa acaggaaagg ggctgtctgt ggtgaattac
      601 atcatgagga gaatgcttct gtttatcaga atgacgaggt ttgttaaagg tgttttataa
      661 aaatagtggg gtagtgctgg cacagagatg tcttacaggc attctcccgc aaaaaatgaa
      721 cccgttgctt gttatagtgc cttgcttaat acattgcctg aatccggagt tactttttaa
      781 taattctgtt aatggttatt agctcaccta atatttcgaa gttatcaggt gggcagttaa
      841 tccatttgtt agagccagcg atgatttccc tgtcccagcg ttttattctt tcgccatttt
      901 tttctatgca tatcatgtaa tttgatgggg tcattaattc tttggatact gaaagacatt
      961 cccgaaatga tggtatttca agcaaaactt ccgcgcctga attttcacgg taaaaaatgt
     1021 gccatgttgt gcctttttta ttagttttca cgtgcgtatt ctcataggat aaatataaaa
     1081 ccttgtaaca ttcaggaggt aacaggattt attctgtaag aacaacagac agaacaatga
     1141 tatctgtttt atgtatacga tgtagccagt gatttagtac attgctggct atatcacttt
     1201 tcccattttg tgataagtct gctttgaaac gatggtgtcc ctcctcctgt attgtgaaag
     1261 ttgccacatt tttttcttca tatccctgaa catccccgtc gtgccttata taattgactc
     1321 cattaagaag ttgtcagaat aattatttct gattgcaacg gagttaattt cagtgatggt
     1381 aaagatatcc gtggcaccat aacgcatttt tcatcatcag tatagtggta tgcgtgaaca
     1441 tatatggtgg cattttgcct gatgttgtta aggcttttca gcaggtctac agtcaactcc
     1501 atcgaggtat ccttcaggtt gttatcattc gttgatgatg cgatctgttg aaggcgttgg
     1561 cgacaattaa ttctttttct cttctgaaaa atttacggaa tacaaaaaag atctgttttt
     1621 aactgattgt tactttctga agatattctc tgctccattt gctttccggg taagacgtat
     1681 ggctggttat ggtatggtcg gactgcagcc acagggggat gactgtaata cctctgatca
     1741 ccacagtcag tcatctgtaa atgttaaata cctcacagtt cagtcagtgg attattgtcg
     1801 gtcttacggg atatttgtta aaaaaatact gcatggtgaa tccccctgag cggcggggca
     1861 tatcagtatc gtaggtgttt ggtcacatcc cttatctgtg cgggttcagg tgctgatact
     1921 gaactcaccg ggaggcaccc ggcaccatgc tacataacac tccattacgc tacactcgcc
     1981 cctctccgga ggggttttct ctgcaggatg tgaatatgag atgctgcatc attcactgcg
     2041 aatattgatg gtgagaatct ttaataata
//
LOCUS       DAZGHF010000301         2038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGHF010000301 DAZGHF010000000
VERSION     DAZGHF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.303"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..263)
                     /locus_tag="ACHK3A_005096"
     CDS             complement(<1..263)
                     /locus_tag="ACHK3A_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO8743026.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDP"
     gene            complement(510..1916)
                     /gene="gndA"
                     /locus_tag="ACHK3A_005097"
     CDS             complement(510..1916)
                     /gene="gndA"
                     /locus_tag="ACHK3A_005097"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO8743027.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIV
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          564 a          464 c          460 g          550 t
ORIGIN      
        1 ttaggatcat agtcggttgg agtggcgatg atgacgtaat cagcatcccg ataggcttca
       61 tttttatcta atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg ctgaatttcc
      121 ttatcaacaa taggagatat ccgatcattc agcatagcaa cgcgtgacgg taaaatatct
      181 aatgccacaa cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc gacatagcca
      241 gtaccggaaa tggtgatttt catttcatgc tctcagaatt aacttaactg tgaatcatga
      301 tgtttttagc atcctgataa gagttaaaag ttttaacgct acgttgtaag atggtcaacg
      361 caacgaaaaa caattcagaa tttctttata agaatagctg atatttatta agttaatatt
      421 aagcagtgaa atttagtcta taagataatt ggtaaaaaaa cccggcataa tatacgccgg
      481 gcctcaattt tattgttgat taaatcagat taatccagcc attcggtatg gaacacacct
      541 tctttatcaa tgcgcttata agtatgcgca ccgaaatagt cacgctgtgc ctggatcagg
      601 ttcgcaggca gaacagcggc acggtagctg tcataatagg caaccgcagc ggcgaaggtc
      661 ggaaccggga taccgttctg tactgcataa gcaacgacat cgcgcagcgc ctgctggtag
      721 tcatcggcaa tttgcttgaa gtaaggagcc agcagcaggt tagcgatctg cggattttcg
      781 gcataagcat cggtgatttt ctgcaggaac tgcgcacgga taatgcagcc agcacggaaa
      841 atcttcgcga tttcgccata gttcagatcc cagttgtact cttcagacgc tgcgcgtagc
      901 tgagagaagc cctgagcgta agaaacgatt ttgcccagat acagcgcacg acgaactttt
      961 tcgatgaact cagccttgtc gcctgctgac tgagcttgcg gaccagagag aactttagat
     1021 gcggcaacgc gctgatcttt cagagaagag atataacgtg caaacacaga ctcggtaatc
     1081 agcgacagcg gttcgccgag atccagcgca ctctggctgg tccatttacc ggtacctttg
     1141 ttagccgctt catccagaat cacatcaacc aggtagttac cgtcttcatc ttttttggtg
     1201 aagatatctt tggtgatgtc gatcaggtag ctgcttagtt caccgttatt ccactcggta
     1261 aacgtctgcg ccagttcttc gttggtgaga ttcaggccac ctttaagcag agaataggct
     1321 tcagcaatca gctgcatatc gccgtattca ataccgttgt gaaccatctt cacatagtga
     1381 cctgcaccat cggcaccaat ataggtaacg catggctcac cgtcttcagc cactgcggcg
     1441 attttggtca ggatcggtgc aaccagttca taggcttctt tctgcccacc aggcataatg
     1501 gaaggacctt tcagcgcacc ttcttcacca ccggaaacac cggtaccgat aaaattaaag
     1561 ccttcggcag aaagctcacg gttacgacga atggtgtcct ggaagaaggt attaccacca
     1621 tcaatgatga tgtcaccttt atcgaggtat ggcttgaggg aatcaatagc agcatccgtg
     1681 cctgcacctg ctttcaccat taacaggatg cgacgaggcg tttccagaga ttcaacaaac
     1741 tctttcaccg tatagtaagg aaccagtttc ttgcctggat tttcgacaat cacttcttcc
     1801 gtcttttcac gggaacggtt gaaaatagag acggtataac cacgactttc gatgttgagc
     1861 gcgaggttgc gccccatcac tgccataccg acgacgccga tctgttgctt tgacattgtt
     1921 tactcctgtc agggttttac cgcatgtgcg gtatgagaat aacttaaaaa gtttacttga
     1981 aataaagtat tatatatatg atattgacgg taaaaacaaa gttgtcgaca gactgaat
//
LOCUS       DAZGHF010000302         2010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGHF010000302 DAZGHF010000000
VERSION     DAZGHF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.304"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..656)
                     /locus_tag="ACHK3A_005098"
     CDS             complement(<1..656)
                     /locus_tag="ACHK3A_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8743028.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     A"
     gene            complement(656..985)
                     /locus_tag="ACHK3A_005099"
     CDS             complement(656..985)
                     /locus_tag="ACHK3A_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743029.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(982..>2010)
                     /locus_tag="ACHK3A_005100"
     CDS             complement(982..>2010)
                     /locus_tag="ACHK3A_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8743030.1"
                     /translation="LKEAATKRQLSGQEKSLLAHKDETLEYKRQLAELGDKVEYQKRL
                     NELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPQALAQVTG
                     ALKQTWADEDMLRGDWLAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQNMAAMLT
                     GAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTAIEAAAA
                     NFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGA
                     GSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELRLQLRDG
                     GMLSGSGR"
BASE COUNT          387 a          742 c          453 g          428 t
ORIGIN      
        1 gccaccatgc cgcgcatctc acacccgcgc atgcatttac tgcatctgtc cttccggata
       61 tccgtggtgg ggttgtcgaa ctcatccgcc actgccggac cgttataccc gcactcatcg
      121 ccccggtaat cccacataca ggtgttcgcc agcatgatgc gaccgggaaa cagcgcaccg
      181 tccgtctccg tcggtgtcgc cagcacaaac gaggccgtca tggccgtcag cgatgacatc
      241 tgctccacca cccaccggtc cgtcagctcc tgctccgggt ctgcctcagg attgcctgcc
      301 acaaaattca ccgcatccag aaaacgcgca tacacccggc gacggaccac cgtggcaccc
      361 accaggctct gcaaatcctc cgccatcccg gtgaccagtc cgaaaagatt ggacaccgtc
      421 agcgacgggc gggcactgct gccctttccg ttcatctcaa agccgctgcc ctcaatcggg
      481 tacgcctcat acttccgccc ctgccaggtc accggctccc ctttttcatt cagctcattg
      541 cagaaaaaat accgctcacc gccctgcacc gtcaggtcga tttcccagag caccacccgc
      601 ggtgactgct ctgacttaac cgactcgttc aggctttctt cgtgaatatc ctgcatcagt
      661 tcaccacctg cttaaactcc gcgctgaact caacgcgcaa catcccgacc cgcgcagacc
      721 acccggcgca ggtcaccttt atctgccggt atgcataggg tggcttccac aaaaatgcct
      781 tccagccccc gtgctctgcc agaaacgctt ccagatgccg ggcctcctcc cgggtcacgg
      841 aaagcgtcac ccggtatgtt ttcaggtcag cattcagccc tgccgccata cgctgtgagt
      901 acccgtcacc aaaacgcact tcacgcaccg acggctgcga gttcacctcc atatccggct
      961 tcactttcca gcgaaaggtt ttcatcgccc gctccctgat aacataccgc catcacgcaa
     1021 ctgcagccgg agttcatcct gcgccccctt acgggccatg tcatacaccg ccttcatcag
     1081 ctgcggcccc gcctgtccgt ttataccgtc gttctgaatc accacgtgat tgttctgatt
     1141 aaaattaata ccttccgccc gccgcatctg cgccggactt ccggcaccac ccacataacc
     1201 accttccgca tagccgcgca tcagacggta aagattcccc acacctatcc ggctggttgc
     1261 ctctttcgtg aaaacaaact ccccgcggtg aactatcccc gcaggctcat atttgccgcc
     1321 cgtccccgta aatcctccgg tcgcgaaatg gaagttcgcc gccgcagcct caatggccgt
     1381 ccccgaggaa gcagatgcac caccaccgaa agcaccacca atggcgctgc cgatacgccc
     1441 gacaattccc accatggcct gtttaagcag gatttctgtc atcatggaca gcaccgaacg
     1501 ggtgaatccc cgccagtctg cctctgcacc ggtcagcatc gccgccatat tctgtgcaat
     1561 accatcaaag gtctgcgtgg ccgcactttt aacctgcgaa aaactgtccg tcgcactttc
     1621 cgcccactcc ccccagccgg acttcagccc ggccagccag tcaccgcgca gcatgtcttc
     1681 atccgcccat gtctgtttca gtgccccggt gacctgtgcc agcgcctgcg gattatcgcc
     1741 gtacacgtca cgaagacgct gctcttcaga ctcccgctgt gcctgacggt cggtgagacc
     1801 acgggctttt gcgctgatgg cggcctgctt cgcgctctgc tgctcttcaa accgcaccgc
     1861 ctgctgtgcc agctcattca ggcgtttctg gtattcaacc ttgtcgccca gctcagccag
     1921 ctggcgtttg tactccagcg tctcgtcttt atgcgccagc agggattttt cctgcccgga
     1981 taactgtcgt ttcgtggcag cctctttcag
//
LOCUS       DAZGHF010000303         1996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGHF010000303 DAZGHF010000000
VERSION     DAZGHF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.306"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(248..1120)
                     /locus_tag="ACHK3A_005101"
     CDS             complement(248..1120)
                     /locus_tag="ACHK3A_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO8743031.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT          498 a          444 c          519 g          535 t
ORIGIN      
        1 gagatccggc tgacggaagt ttctgaattg tggttctgtt gttccctgaa tctcatgtct
       61 gtgtgtatct gcgcacgttg ctaacaggag ttattattag cataacgatc gatattaacg
      121 atcggtgaaa tgggtatcga tcgttttatt ctattatcta gctattgcac ggacaagaga
      181 gacagtaacg aaaacagcgc ttttgtgagc agacccagag gcagggagga catcatccac
      241 gacaggttca gaagaaaatc cagttccata ccgcacgggc aacagagacc accgtgtcac
      301 gaacagcgcg cagggccgta cggacaacag aggcaacaca gacgctctcc gctgtgtcaa
      361 atatccggtc caccgcaccg gtaaactgtt cacgggcctg agagcggaca gattccgtgc
      421 gcagcccgtc ctgcagtcgg gtcatcaggg gactggcggc atggtcggga agcgctgtca
      481 tgagcgcact gaccagcgta tccagttccc agccggtgcg ggccgatacg gccacaaccg
      541 gatgtacggg ccggaacaga cggaataccg cctccgtttt ctcacgaatg ttctgttcct
      601 gtgcgggaga aggctgaatg ccggccatat cccattcatg gcagggctcc gttttgtcgg
      661 cctgcgtcac cacaaacagc acctgctgat gtccgcactg caggatgtgt cgccagaaat
      721 actcatccac agacagggcc cggtcatcgg ctttaatcag ccacagcacc aggtcaagtt
      781 caggcagaat gtcacggtac agggcctcat actctgcatc cctgtcccgg ctctcgccca
      841 ccccgggcag gtcagtgata accatgctgt gaccgtgacc actcagacgg aagcgccgta
      901 cttcccgggt gccggcgtga acatcactga ccggggtgac ctccccctga aacagtgcat
      961 tacagagtga ggatttaccg gccccgcttt tacccataat gccaatcacg ggttcgtgac
     1021 tggtgagttt acgcagatgt tccaggatgt gacgggaaag tgagtaaggc agggaggaaa
     1081 gcggtttttc aattgcctca atggcatcag acggattcat acatgtattc ccggtgaaaa
     1141 aagacaaaac cccgcgcacc ggaaaggtgg cgggggatcc acggagataa tatctgtctg
     1201 aaattgtttt gaatcgatgg gattgttgcg tattaacatg aaggagatca ggacttatcc
     1261 gttactgggt gatctccttc catgaaaata cgcggttatt gttaaacagc aggctgatta
     1321 ctccgtaaag gagaaaaata ttcatcttgc agcctgatta tcaggtgtgt ttatgtttac
     1381 tgaggtgagt atttttcagt atattgtcgg ggcagtcagt gggagatatt aaatttacgt
     1441 ttagagttat cttgcttctg ctgtgtattt ttataagagt gcgcacgtaa tgatatctgt
     1501 tgataccatg ttgttaagtg tttcccttcc gggtcatgcg tacttgcaaa atggcatctg
     1561 aatcgatcac ggtttggcca aaccgtgatc gaatagagag gaatacgcac gtaatgatta
     1621 aatgcttttg ttacaccatt cttccggcat gaatatttat ccagttccct tttttatttg
     1681 tgtagtgata ctgtcggaat agcgttcagt aatgtgaagt gtcctgttaa aaatctttaa
     1741 taaatgtgtc accctggtca ggaaatatct gctgagatat tattagttgg tggtgttttt
     1801 actgagtaat agctggcttt taattcctga actgaaatgc tgtcgctcgt cagggtttaa
     1861 ttaaaactgg atgatgatgt gaaaatgaag gctggttttt gtgtttgtcg aggaagtgtt
     1921 gtttttatcc attctcatca ttggctggct gcgattatac agaagtattt gaagaaaaaa
     1981 tggtgcattt tatctg
//
LOCUS       DAZGHF010000304         1954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGHF010000304 DAZGHF010000000
VERSION     DAZGHF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.307"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(617..1708)
                     /locus_tag="ACHK3A_005102"
                     /pseudo
     CDS             complement(617..1708)
                     /locus_tag="ACHK3A_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
BASE COUNT          609 a          447 c          354 g          544 t
ORIGIN      
        1 taaagattct caccatcatt cgcagtgaat aatgccgcat ctcatattca tatcctgcag
       61 agaaaacccc tccgaagagg ggcgagtgta gcgttaatgg agtgttatgt agcatggtgc
      121 cgggtgcctc ccggtgaaca gactgttcgt gataccagtc agcaacatag gaaattaatg
      181 gtatcacccc accgcacagg gggattcacc atgcctgcct tttttagcaa attcatcaga
      241 aaccggacaa catgcttatt tatgaaatga ggtgcttttc acatttatat aaaatcatta
      301 taacgataat cctaaaaatc aatgtaatta tcatcttcag taactttctg tattacacca
      361 tacggaaatt gatttttgac ggtaaagata caaggggaag tatgtctgta aggagtaccc
      421 tgaataatgg acaggcatgc atcgtggctg ttcccctgaa attatcagta caaccagtca
      481 gctcataatc cctgtctctt tttccgggga gccctcaggg ggactttccc ttatcccccc
      541 agtttctgga gagacgtaaa aaagaattaa tcatatgtca ggtatctgaa tatgtcccgt
      601 aattaatata tttacgtcag gattttttct gcccgccagg agtatgaaca tcttccgccg
      661 ttgagtctgt catatccatt tttttctgtg ccagtacttc atatgatttt tctttttcca
      721 gactctcctg ctgcatcccg tgagccggag catatacaag ctcagccatc actggaaaat
      781 gatctgaacc catatccggt aaacggcgta aacttttaag cagaaaatgc tggctgtgaa
      841 atacatggtc aagcggccat cgcaggaaaa aataatggct gtgaaacgtg ctaaaaaaac
      901 cacgtccccg gcgtggatcc agcagtccac ttattttcat gaataaacga gtggtcgcag
      961 accacgcaac atcattcaga tcccccgcta atattacagg taattcagaa tctgcaacac
     1021 ttttcccgac cattaaaagt tctgagtcac gatcagttga ctcatcactt tcagtcgggc
     1081 tcggtggaac aggatgaaga caatgtaatc ttattctctg accttccggc atatgaacta
     1141 tcatatgcat ggagggaatt tccttgtcca ctaaatactg tatttctgca tcacttaaaa
     1201 tcatccttga atatacatgc atgccatata aattttcttt cgggcatttc agggagtatg
     1261 gaaacacttc atccagaggt gatagtttct gttcccacca taagtttgtt tcaagaagaa
     1321 caagtatatc aggtttttct ttatttataa gttcaagcaa aggcatatat tttttattgg
     1381 tcataaggac attgaatacc agtatactta aacccggaaa tccatcagag tcctttattt
     1441 actttacctg cttcttgcca attactgtat aaggaattat ccataccaac tggtaagcga
     1501 cacaaattaa acttattatc acaacaaaca actctgcaaa tgagtcaaga aaaacaacag
     1561 acagaaaaac attcaaagta cacagcaaaa gtatctgtag tcggggaaaa tcccataccc
     1621 tgacaaccca tgatgtatta ccggaaacag ggataaaagt tatgactgcc agaaggatag
     1681 cagtaaaaat aaaaacacaa gttatcacaa atcactcctt gttctgaacc ggcacacaaa
     1741 actgtcatat acgtttcaaa agtaaaaaca cactgctgcc acaagattca cgacgtaacc
     1801 gggcagcata tcctgattac ggacaatcca tgaaaccgcc tcgccagaag cgtccatcac
     1861 atccggtttt tccctgtttt atattccacg aaccatttta ttttcaggaa tctccgggcc
     1921 tttatcccgc atcatataca cagctatgac aacc
//
LOCUS       DAZGHF010000305         1939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGHF010000305 DAZGHF010000000
VERSION     DAZGHF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.308"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1939
                     /locus_tag="ACHK3A_005103"
     CDS             <1..>1939
                     /locus_tag="ACHK3A_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFO8743032.1"
                     /translation="YPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKA
                     AQRSITAPDRIGEGKTWVYQRDTMVERGDNRGVHQYGPAEHFTHIISDKPSPKDKYVA
                     YAINIPDYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEII
                     TPKKPGEAILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYW
                     FHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYP
                     SADIRAKISKDIESGIPGYWQGQVLIPFTIDPVTKERKQIHPEDIHLTAKDFETSKDN
                     ISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSK
                     HEQIERSITPPLTIYNERLHNTNLFKPIYHEKAVMNYMEYLVTCQLDEEQMSSLLYER
                     DDLSAIDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSKRRILININHDFPNNGNL
                     FNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLN
                     KGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVII
                     NKGNEEVEGTWPNKLQPGIYKN"
BASE COUNT          680 a          382 c          361 g          516 t
ORIGIN      
        1 ttatcctcag gatttaaaag acagagaaat tatatcaact tttcacgcag aaaaagtcat
       61 taatgatctg ttaaaaaacg attataaaat ttccctttct ccagatacaa cttaccgaga
      121 gctgaataaa gctgcacagc gtagcattac agcgccagac aggataggag aagggaaaac
      181 atgggtttat caacgagata caatggttga aagaggtgat aacagaggcg ttcatcagta
      241 tggtccagct gaacatttca ctcacattat atctgacaaa ccatccccaa aagataagta
      301 tgttgcatat gctattaaca ttcctgacta tgagctagca gccgatgtat ataatattaa
      361 cgtgacgtca ccttccggac agcaagaaac atttaaaata ctaatcaatc cagaacatct
      421 acggcaaaca cttgagcgta aatctcttac tgctgttcag aaatcacaat gtgaaatcat
      481 cacccccaaa aaacctggcg aagcgattct tcatgctttt aatgccacct accagcaaat
      541 cagggaaaat atgtctgagt ttgcacgttc ccattatggg tatatacaaa ttcctccagt
      601 gacaactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaaaggtt actggtttca
      661 cgcttatcaa cccgaagatc tttgtaccat ccataatcca atgggagatt tgcaggattt
      721 tatcgcattg gttaaagatg ctaaaaaatt tggtatcgat atcattcctg attatacctt
      781 taactttatg ggaattgggg gtagtggtaa aaatgaccta gattatccct ctgctgatat
      841 acgagcgaag atcagtaaag atatagaaag tggtatccct ggctattggc aaggtcaggt
      901 tttgattcca tttactatag atccagtaac aaaagaacgt aaacaaatcc atccagaaga
      961 tatacatctc actgcaaaag actttgaaac aagtaaagat aacatctcta aggatgaatg
     1021 ggaaaacctc catgcattaa aagaaaagcg tttaaatgga atgcctaaaa caacccccaa
     1081 aagtgaccag gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa aatatggcgt
     1141 acgaggttta cgttacgatg cagcaaaaca ctcaaaacat gaacaaatag aaagatcaat
     1201 aactccaccg cttacaattt ataatgagcg attacacaat actaacttat ttaagccaat
     1261 atatcatgaa aaagccgtta tgaattacat ggaatacctg gtaacttgtc agttggatga
     1321 agaacaaatg tcatcgctgc tttatgaaag agatgattta agcgccattg atttttcatt
     1381 gctcatgaag acgataaaag ccttttcatt tggtggcgat ctccaaaccc ttgcatcaaa
     1441 accgggttca acaatctcaa gcatcccgtc aaaaagacgg atattgatta acattaacca
     1501 cgattttcct aacaatggca atcttttcaa tgactttcta tttaaccatc aacaagatga
     1561 acaattagca atggcatata tggccgctct cccgttcagc aggcctttag tttactggga
     1621 tggccaagta ttaaaatcaa cgactgaaat taaaaattat gatgggtcga cgcgtgtcgg
     1681 cggtgaggcg tggcttaata aaggttgctc tacctatcag cagctctaca atgaattcca
     1741 cgcattatat atagataaag caggaatatg gagcgcattt gagggtgtat ttgcaactaa
     1801 aaacgttctg gcctttagtc gtggggattc tgtgaacatt aatcactctc ctcatgatgg
     1861 actagttata ataaataaag gaaacgaaga agttgaaggt acctggccta acaaattgca
     1921 acctggaata tataaaaac
//
LOCUS       DAZGHF010000306         1923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGHF010000306 DAZGHF010000000
VERSION     DAZGHF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.309"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..935)
                     /locus_tag="ACHK3A_005104"
     CDS             complement(<1..935)
                     /locus_tag="ACHK3A_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8743033.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPVRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYV"
     gene            complement(1003..>1923)
                     /locus_tag="ACHK3A_005105"
     CDS             complement(1003..>1923)
                     /locus_tag="ACHK3A_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HFO8743034.1"
                     /translation="QLAALMAGQLLPGEAGAWLATVPGALQNLAGAVWHQHPEMALIR
                     TVLQEAMEEVVSPFAAGDVAEVPVAEVVPVLLQSAAISAESVIQPLPVVPSPVEDAEN
                     EGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPDKESSIELPEESGC
                     VTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLSVNSVDSNVHVVSG
                     YVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFFQARLYSDAEHSGR
                     YKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
BASE COUNT          470 a          583 c          385 g          485 t
ORIGIN      
        1 tcaacatacc ccatggtcgt tgtaagactc ctgtgtccca gtatggattt gaccagatgt
       61 aaattacgtt caggatattt catcagtctg gttgccagcg tatgtctgaa acgatgtggt
      121 gaaacaggaa aaccacattc tcgcgaaagt cgccggaaaa atgaacgtac ggtctgatgc
      181 gcccgaacgg gacagtactg atatctgtcg gcagagatcg gctcactgaa acgacagacg
      241 tcaaaaagat agtcatcatc acctgccccg cactgacggg cccgctccag cagtttttcc
      301 atcgatacct tcaggggagc caccagagga acgccccatt ccctgtgagt tttacttccc
      361 tccagcttaa gatcaattat gctttcatcc ggacagacat ccctcacacg gatatgcttc
      421 agttgattaa agcgcattcc ggtataacgc agaacatcca gaactgtcag ccagtaccag
      481 gcagggtata tagcattgcg tcgacgacta ttcaccatgt tctgctcttc cgcttctgca
      541 aacctctgca caaccaggta aaccctgagc atctgattgt cactcagtgt ctttttcttt
      601 ttttcagtac tcgcaactga tgcttccacc aacgggtttt ctgtctgagg cagcagtttc
      661 atcttgatcc cgaatcccat aattgcccgc aagtgggcca ccttgttatt ccaggtcacc
      721 tccctgcgct tctgctcttt caggacataa cgccggtatc gctgaacatc acggacagtc
      781 acctcccccg gcaaaatatc accgataaat tccctgaata ccctgacaac tttgcagtag
      841 ctccactctg tatccggccg aagattcttt gaaaaaaagt actcttccag cagttgttcc
      901 caggtgattt ttgtgacgga atcgtcattc tgcattatat attccccttg tttactgtgc
      961 tcccgcactc tccggtcaaa ttctgcacat catcgccttt actcacccga aaacaataag
     1021 cgggctgtct ccgggaaccg taatattccc gtacagaaac ctgcccttaa ccagatatcc
     1081 cgaaaccatt ttgtaacgtc cggaatgttc tgcgtcacta taaagacggg cctgaaaaaa
     1141 acgctctcct gaacgtacct tatggatacc tgcccgttca aagagcgact gaacataacg
     1201 acgatcctgc ccggaaccac tctccttcag aaaacgataa aaaatccccg gggcacacag
     1261 aaaaacatat ccggaaacaa catgaacatt tgaatcaacg ctgttaaccg aaagtgaccc
     1321 ctccctcagc ccctctttta accatctgat aaattcactc tctaccctgc tatcctctgt
     1381 cagggaaaac tctgccgttt tttccactgt ttccgggata cggtttccct gattttctgt
     1441 tacacatccg ctctcttccg gtaactcaat actgctttct ttatccggcg gcacgcctga
     1501 cgctatctgc cctccctcat ccgacaaatc agcagccgaa aacagcgtaa ggagcattcc
     1561 ggtatcagca tcagtttcgt tcccctcatc cacctcctgt tccgctgtcc ggccctcatt
     1621 ttcggcatcc tccactggcg acggtacaac cggcaacggc tgaatgacgc tttctgctga
     1681 aattgctgct gactgcagca gtaccggaac aacctcagcc accggaactt cggccacatc
     1741 tcctgcagcg aagggactca cgacttcctc catagcttcc tgtaatactg tacgtatcag
     1801 cgccatttca ggatgctgat gccagactgc tccggcaaga ttctgtaatg cccccgggac
     1861 agtggcaagc caggctcccg cctctccggg cagcagttgt cccgccatca gggctgccag
     1921 ttg
//
LOCUS       DAZGHF010000307         1902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGHF010000307 DAZGHF010000000
VERSION     DAZGHF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.310"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            110..862
                     /locus_tag="ACHK3A_005106"
     CDS             110..862
                     /locus_tag="ACHK3A_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO8743035.1"
                     /translation="MTHKPYISPSLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYINDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDAFQAAGFTWRGIVAWNKGRASRTPHTGYFRHQCEYVVWGSKGALG
                     KSPSGPFDGCITHHTRPQEKQHPTGKPESLMDELVRVCEPGGLILDPFMGSGTTGVAA
                     LRRGCRFTGIELNAHYFSVAQARLTGTEQRND"
     gene            862..1200
                     /locus_tag="ACHK3A_005107"
     CDS             862..1200
                     /locus_tag="ACHK3A_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO8743036.1"
                     /translation="MKLSEKQQLFAVMIADLIHWAQEHGYRLTFGEAYRTPEQAALNA
                     KSGKGIRNSLHTLRLAVDFNLFINGKYQADTDAYRPLGEYWESIGGTWGGRFSRADGN
                     HFSLEHNGVK"
     gene            1197..1583
                     /locus_tag="ACHK3A_005108"
     CDS             1197..1583
                     /locus_tag="ACHK3A_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743037.1"
                     /translation="MMKQLAGCLVILFAAMWAGWLAHDWHDARVALAEQEAMEATRRA
                     VTEAGQQSARILEKRLEALKGNEIHTERVIHTETVRPVFRNVCATDDYVRLFNARAEA
                     AERTLAGKPADGMPEDTAETGRPDGR"
     gene            1683..>1902
                     /locus_tag="ACHK3A_005109"
     CDS             1683..>1902
                     /locus_tag="ACHK3A_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFO8743038.1"
                     /translation="MTTKNTGTIILSVAGVTLSFEPNKTAFNNLINEITMTNKVAPMS
                     TYLNRIVTPASRDALADLIARYPGCEMQI"
BASE COUNT          443 a          555 c          569 g          335 t
ORIGIN      
        1 ctggtagtgg tcggtctggc gtgcgcgctg ggcattatgg gcagcgcctt tattgaagaa
       61 tgcttttgcc gtctggcaga ccgctacctg aagcagaagg aacgcagaaa tgacccataa
      121 gccttacatt tcgccttccc tgagcctgta tcacgcgcag gcgcatgacg ttctgcggat
      181 gctgccggaa aacagtgtgg acgcgctcat cacagacccg ccatacagtt caggtgccac
      241 acacaaggcg gggcggacat gccagggcag ccacgccaaa tacatcaacg atgaagatct
      301 tcaccgcttt gaaggcttcg aaggcgagaa catggacgcc cgttcatggg aatactggac
      361 gcatctgtgg atgacacagg catggcgcac ggtacgaccc ggtggttacg ccctggtctt
      421 caccgactgg cggcagttgc ccgcgctgac ggacgcattc caggccgccg gctttacctg
      481 gcgggggatc gtggcatgga acaagggacg ggcctcaaga acaccgcata ccggttattt
      541 tcgtcaccag tgcgaatatg tggtctgggg cagtaaaggc gcgctgggca aatccccttc
      601 cgggccgttc gacggctgca tcacgcacca cacacgccca caggagaaac agcacccgac
      661 aggtaaaccg gaatcgttga tggatgagct tgtgcgcgtg tgtgaacccg gagggctgat
      721 cctcgatccg tttatggggt ccggcaccac gggcgtggcc gcccttcgcc ggggctgccg
      781 ttttacgggt attgaactga acgcccacta tttcagcgtg gcacaggcgc gccttacggg
      841 gacggaacag aggaatgact gatgaaactg agcgaaaaac aacaactgtt tgccgtcatg
      901 attgccgatc tcatccactg ggcacaggag catggctatc gcctgacctt cggcgaggca
      961 taccgcacgc cggagcaggc cgcactgaat gcgaaaagcg gaaaaggcat tcgcaacagc
     1021 cttcacacgc tgcgcctcgc ggtggacttc aacctgttca ttaacggcaa ataccaggcc
     1081 gataccgacg cctacaggcc gctgggggaa tactgggaat ccatcggcgg cacatggggc
     1141 gggcgcttca gccgggcaga cggcaatcat ttcagccttg agcacaacgg ggtgaaatga
     1201 tgaaacagct ggcgggctgc ctggtgatcc tctttgccgc catgtgggcg ggctggctgg
     1261 cccatgactg gcatgacgcc agggtggcgc ttgccgaaca ggaagccatg gaagccacgc
     1321 gacgtgcggt aacagaagcc gggcagcagt ccgcccgcat actggaaaaa cggctggagg
     1381 cgttaaaggg caatgaaatt cacactgaac gcgttattca cacggaaacc gtccgcccgg
     1441 tattccgcaa tgtgtgcgct actgatgatt acgtccggct gttcaacgcc cgtgctgaag
     1501 ccgctgagcg taccctggca ggaaaaccag ctgacggaat gcccgaagac actgccgaga
     1561 ctggcaggcc cgacgggcgc tgattttgat gcggcactgc gaaccctcac gagccgctac
     1621 accgaatgcg ccgccaggca taacagcctg gtggaaacca tacgaaacac ggagaaactg
     1681 caatgaccac gaagaacacc ggcaccatca tcctgtccgt tgcgggcgta accctgagct
     1741 ttgaaccgaa caaaacggca tttaacaacc tcatcaacga aatcacgatg accaacaagg
     1801 tggcgccgat gagcacctat cttaaccgca tcgtgacgcc tgccagccgc gacgcactgg
     1861 ctgacctgat tgcgcgttat ccgggctgtg agatgcagat cg
//
LOCUS       DAZGHF010000308         1902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGHF010000308 DAZGHF010000000
VERSION     DAZGHF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.311"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            110..862
                     /locus_tag="ACHK3A_005110"
     CDS             110..862
                     /locus_tag="ACHK3A_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO8743039.1"
                     /translation="MTHKPYISPFLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYLNDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDAFQAAGFTWRGIVAWNKGRGSRTPHTGYFRHQCEYVVWGSKGALG
                     KSPSGPFDGCITHHTRPQEKQHPTGKPESLMDELVRVCEPGGLILDPFMGSGTTGVAA
                     LRRGCRFTGIELNAHYFSVAQARLTGTEQRND"
     gene            862..1200
                     /locus_tag="ACHK3A_005111"
     CDS             862..1200
                     /locus_tag="ACHK3A_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO8743040.1"
                     /translation="MKLSEKQQLFAVMIADLIHWAQEHGYRLTFGEAYRTPEQAALNA
                     KSGKGIRNSLHTLRLAVDFNLFINGKYQADTDAYRPLGEYWESIGGTWGGRFSRADGN
                     HFSLEHNGMK"
     gene            1197..1583
                     /locus_tag="ACHK3A_005112"
     CDS             1197..1583
                     /locus_tag="ACHK3A_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743041.1"
                     /translation="MMKQLAGCLVILFAAMWAGWLAHDWHDARVALAEQEAMEATRRA
                     VTEAGQQSARILEKRLEALKGNEIHTERVIHTETVHPVFRNVCATDDYVRLFNARAEA
                     AERTLAGKPADGMPEDTAETGRPDGR"
     gene            1683..>1902
                     /locus_tag="ACHK3A_005113"
     CDS             1683..>1902
                     /locus_tag="ACHK3A_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFO8743042.1"
                     /translation="MTTKSTGTITLTVAGVTLTFEPNKTAFNNLINEITMTNKIAPMT
                     TYLNRIVTADCRDALADLMDRYPGCEMQI"
BASE COUNT          450 a          555 c          561 g          336 t
ORIGIN      
        1 ctggttgtgg tcggtctggc gtgcgcgctg ggcattatgg gcagcgcctt tattgaagaa
       61 tgcttttgcc gtctggcaga ccgctacctg aagcagaagg aacgcagaaa tgacccataa
      121 gccttacatt tcgcctttcc tgagcctgta tcacgcgcag gcgcatgacg ttctgcgaat
      181 gctgccggaa aacagtgtgg acgcgctcat cacagacccg ccatacagtt caggtgccac
      241 acacaaggcg gggcggacat gccagggcag ccacgccaaa tatctcaacg atgaagatct
      301 tcaccgcttt gaaggcttcg aaggcgagaa catggacgcc cgttcatggg aatactggac
      361 gcatctgtgg atgacacagg catggcgcac ggtacgaccc ggtggttacg ccctggtctt
      421 caccgactgg cggcagttgc ccgcgctgac ggacgcattc caggccgccg gttttacctg
      481 gcgggggatc gtggcatgga acaagggacg gggctcaaga acaccacata ccggttattt
      541 tcgtcaccag tgcgaatatg tggtctgggg cagcaaaggc gcactgggca aatccccttc
      601 agggccgttc gacggctgca tcacgcacca cacacgccca caggagaaac agcacccgac
      661 aggtaaaccg gaatcgttga tggatgagct tgtgcgcgtg tgtgaacccg gagggctgat
      721 ccttgatccg tttatggggt ccggcaccac gggcgtggcc gcccttcgcc ggggctgccg
      781 ttttacgggt attgaactga acgcccacta tttcagcgtg gcacaggcgc gccttacggg
      841 gacggaacag aggaatgact gatgaaactg agcgaaaaac aacaactgtt tgccgtcatg
      901 attgccgatc tcatccactg ggcacaggag cacggctatc gcctgacctt cggcgaggca
      961 taccgcacgc cggagcaggc cgcactgaat gcgaaaagcg gaaaaggcat tcgtaacagc
     1021 cttcacacgc tgcgcctcgc ggtggacttc aacctgttca ttaacggcaa ataccaggcc
     1081 gacaccgacg cctacaggcc gctgggggaa tactgggaat ccatcggcgg cacatggggc
     1141 gggcgcttca gtcgggcgga cggcaatcat ttcagccttg agcacaacgg gatgaaatga
     1201 tgaaacagct ggcgggctgc ctggtgatcc tctttgccgc catgtgggca ggctggctgg
     1261 cccatgactg gcatgacgcc agggtggcac ttgccgaaca ggaagccatg gaagccacgc
     1321 gacgtgcggt gacagaagcc gggcagcagt ccgcccgcat actggaaaaa cggctggagg
     1381 cgttaaaggg caatgaaatt cacactgaac gcgttattca cacggaaacc gtccacccgg
     1441 tattccgcaa tgtgtgcgct actgatgatt acgtccggct gttcaacgcc cgtgctgaag
     1501 ccgctgaacg taccctggca ggaaaaccag ctgacggaat gcccgaagac actgccgaga
     1561 ctggcaggcc cgacgggcgc tgattttgat gcggcactgc gaaccctcac gagccgctac
     1621 accgaatgcg ccgccaggca taacagcctg gtggaaacca tacgaaacac ggagaaactg
     1681 caatgaccac gaagagcacc ggcaccatca ccctgaccgt cgcgggcgta accctgacct
     1741 ttgagccgaa caagacggca tttaacaacc tcatcaacga aatcacgatg accaacaaga
     1801 tagcgccaat gaccacctat cttaaccgta tcgtgaccgc tgactgccgg gatgcactgg
     1861 ccgacctcat ggatcgttac cccggctgcg agatgcagat cg
//
LOCUS       DAZGHF010000309         1808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGHF010000309 DAZGHF010000000
VERSION     DAZGHF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.312"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..274
                     /locus_tag="ACHK3A_005114"
     CDS             <1..274
                     /locus_tag="ACHK3A_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8743043.1"
                     /translation="PPPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITRELDALG
                     VQLHESAVVSVRHGARVRLADGRTSYWNDSTRRIDITPAPNTEAPQ"
     gene            271..675
                     /locus_tag="ACHK3A_005115"
     CDS             271..675
                     /locus_tag="ACHK3A_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743044.1"
                     /translation="MTRFYCAMQRQRFDELMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTTIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLASINRKAAIARYVHSAAGRQSRR"
     gene            724..894
                     /locus_tag="ACHK3A_005116"
     CDS             724..894
                     /locus_tag="ACHK3A_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743045.1"
                     /translation="MNSTRRISDRELTKMIESAQQLLSRQNPVVEETWWENLLAALTE
                     LRERRNNEGAAK"
     gene            891..1217
                     /locus_tag="ACHK3A_005117"
     CDS             891..1217
                     /locus_tag="ACHK3A_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO8743046.1"
                     /translation="MKTPGMTGKEIILRYLETHARFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTNKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAAFR"
     gene            complement(1283..1546)
                     /locus_tag="ACHK3A_005118"
     CDS             complement(1283..1546)
                     /locus_tag="ACHK3A_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO8743047.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(1584..>1808)
                     /locus_tag="ACHK3A_005119"
     CDS             complement(1584..>1808)
                     /locus_tag="ACHK3A_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8743048.1"
                     /translation="HRFPAGLAGIIPTNGATIPNPETTRETYRKDEVIPLQRKIMNAV
                     NSDPDIPEHLYLCFDVDAGHVAEEKAVPVG"
BASE COUNT          477 a          532 c          498 g          301 t
ORIGIN      
        1 cccgccacct gacacggaaa ccgtgaaaaa cggcggcatc agtgcgcccg ctggcggcgt
       61 tcgcgtgagt gtcgctctga ccgctgagcc acacatcacc gcacaaatca cccgcgagct
      121 tgatgcgctg ggcgtgcagc tgcatgaaag cgccgtcgtt tccgtccgtc acggtgcccg
      181 cgtgcgcctg gctgacgggc gcacctcgta ctggaatgac agcacccgcc gcattgatat
      241 cacccccgca ccgaatacgg aggcgccaca atgacccgct tttattgcgc catgcagcga
      301 cagcgttttg acgaactgat gcgccagcgc acgcccttac tgaccctgaa caacattgcc
      361 acggggccac aaccgggcga tgagctggaa atcacggtcg tcagtcccga tcgcaccacc
      421 acgaccatca tcaccgacat actcaacgga cgaccggaca gcaccaaccg cagctggctt
      481 gtcgccgttc gtccgcgccg tccgcccgga gagccggagc cgctcacgcc tgccggaaaa
      541 atcatgcggc agtaccacga agagcaggca cacaaacaca acgcagaact caccagaaca
      601 ctggcatcga ttaaccgcaa agcggcaatt gcccgctatg tgcattcagc cgctggcaga
      661 caatcacgca ggtaaacaaa gaccatgaac ataaccagaa acagccaact aaaggagcgc
      721 accatgaaca gcaccaggag gatcagcgac agagagctta cgaaaatgat tgagtcggca
      781 cagcagctgc tttcacgaca aaacccggtc gtggaagaaa catggtggga gaatttactg
      841 gcagcactga ccgaactccg ggaacgccgg aacaacgaag gagccgccaa atgaagaccc
      901 caggaatgac cggaaaggaa atcattttac gctacctgga aacgcacgca cgtttcagcg
      961 ccccagaact ggtagaaaaa ctgggcgtga aaagaaccgt cattaatcag gccgccggaa
     1021 aaatggtacg ggaagggttg ctggttgtgg acgggtacac gaacaagata acgcattacc
     1081 ggaagccaac ggaaaaggaa cgacaggaac tggcgcaacg ggctgaacgg cagcaggaaa
     1141 aatcagtcat cgaagcctgc aagcgcagcg aaatcatgaa gcgaattctg tttatctacg
     1201 gcgctgcatt ccgttaaagg agctggtgag tcggggcaaa aattcttttt gccccggcga
     1261 agcagcagac aagcgaagcg cgtcaggaac gccgcgacag tagttcaagc gcataccggc
     1321 gatcttccgg gcgcatggca tccaccagga acttcaccat attgcgaccg gagcgggcac
     1381 cggggctgag ggtatgggaa aaggtcatcg tacagacgaa ggtgtgcccg cattcgacat
     1441 cactgcatgc acagtacaga tccgcaagtt caggggtttt ccagtctgtc ttccggatga
     1501 cagcaggtgc gccacagtca ggacaccggg ctttaaaaat ccgcatctta cctcccgata
     1561 acacgtattc cggggaggac attttacccg accggcaccg ctttttcttc cgccacatgc
     1621 ccggcatcca catcaaaaca aagataaagg tgttccggta tatcgggatc gctgttcacg
     1681 gcgttcatga ttttacgctg aagcggtatc acctcatcct tgcggtacgt ttcacgcgtc
     1741 gtttccgggt tagggatggt ggccccgttc gtggggataa tccccgcaag accggcagga
     1801 aagcggtg
//
LOCUS       DAZGHF010000310         1804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGHF010000310 DAZGHF010000000
VERSION     DAZGHF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.313"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..596
                     /locus_tag="ACHK3A_005120"
     CDS             <1..596
                     /locus_tag="ACHK3A_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO8743049.1"
                     /translation="PEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWL
                     VCRGEIHRFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNL
                     YLDNLAENGFYRVSPSCAQAGDILLCCFGSSVPNHAAIYCGNGELLHHIPEQLSKRER
                     YSEKWQRRTHSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            593..1174
                     /locus_tag="ACHK3A_005121"
     CDS             593..1174
                     /locus_tag="ACHK3A_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8743050.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAC
                     LHEQLGEGAVIHIVPRLAGAGGNGVFQVVLGVAAIVGSFFTGGATLAAWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          443 a          404 c          554 g          403 t
ORIGIN      
        1 caccggaaga ctggctgcag gcacagatgc agggggagat tgtggcgctg gtccacagtc
       61 atcccggtgg tctgccctgg ctgagcgagg cggaccggag gctgcagata aagagtgccc
      121 tgccctggtg gctggtctgc cggggtgaaa ttcaccggtt ccgctgtgtg ccgcacctga
      181 ccggacggcg ctttgaacac ggtgtgacgg actgttacac cctgttccgg gatgcatacc
      241 atctggcggg gataacgctg ccggattttg tgcgtgagga tgactggtgg cgcaacggcc
      301 agaacctgta cctggacaac ctggcggaaa acggctttta ccgggtgtct ccgtcctgtg
      361 cacaggcagg cgatatcctg ctgtgctgct ttggttcatc ggtgccgaat catgccgcca
      421 tttactgtgg caacggtgaa ctgcttcacc atatacctga acaactgagt aaacgggaga
      481 ggtattctga aaaatggcaa cgacgaacgc attctgtctg gcgtcaccgc cactggtccg
      541 catctgcctt cacggggatt tacaacgatt tggtcgccgc atcagcctgt atgtgaacac
      601 ggcagcggag gccatccgtg ccctgtcgct gcaggtgccg ggattccgct gtcagatgaa
      661 cgaaggctgg tatcagatac gtattgccgg tgaggatacc gcgccggagg cggtgtatgc
      721 ctgtcttcac gaacaactgg gtgagggagc ggtcatccac attgtgccgc gactggccgg
      781 agccggggga aatggtgttt ttcaggtggt gctgggggtt gcagcgattg tcgggtcatt
      841 tttcactggt ggtgcaacac tggcggcatg gggcgcagcc ctgagtgccg gagggctgac
      901 tgccaccacg atgctgttct cactgggtgc cagcatgata ctgggtggtg tggcccagat
      961 gctggccccg aaggcaaaga cgccggagta caaaagtacg gataacggta aacagaacac
     1021 gtatttttcg tcactggaca acatgattgc ccagggtaac ccgatgccgg tgccttacgg
     1081 tgaaatgctg gttggttcac gacggatatc ccaggacatc agcacccgtg atgagggcgg
     1141 agacgggaaa gtggtggtta tcgggcgggg ataaaaataa aaaaaatccc gcagagttag
     1201 cggagctgcg ggaaataacg atgaagatta acgttatgga gttatttttt tgggcagaaa
     1261 aacattaacg cagcgaaatt ataagcgcta catggatttt gcgaaaatgt gaagaaattc
     1321 agaaattttt tgctgcagag gtgcagggag cttgccgggt atttgtggca gaagacagaa
     1381 aacatcatgc cggggttaag ggataaaaaa atcccgcaga gtccggagac tgcgggacag
     1441 aaccgatgaa gattaacgtt caaggagttt tgtttttatt gacccgaaaa aactgtaacg
     1501 ccccggaatg atatctgcca cggggacgta cagaaaatgt gaagaaattc agactttttc
     1561 tgtcgcaggt ttacagaacg gatgaccgcc agggtaccag tggcgtaaat gagcaggatg
     1621 ttgtggtgtg gctgaagcaa aaaaatctcc cgtaatatcg ccggatactg acgggagacg
     1681 aatagtcacc ttaaggaggt ctgataatta ttgcttttat tgagcaaaaa aactgtaacg
     1741 ctgcgtcatt atagtggcta caggcaattg ccggaaatgt gaagaatttc agaaatttta
     1801 ttcc
//
LOCUS       DAZGHF010000311         1747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGHF010000311 DAZGHF010000000
VERSION     DAZGHF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.314"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..193)
                     /locus_tag="ACHK3A_005122"
     CDS             complement(<1..193)
                     /locus_tag="ACHK3A_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743051.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCMIR
                     SMNKTCEKAYEYVDALRTNK"
     gene            complement(190..381)
                     /locus_tag="ACHK3A_005123"
     CDS             complement(190..381)
                     /locus_tag="ACHK3A_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743052.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(383..760)
                     /locus_tag="ACHK3A_005124"
     CDS             complement(383..760)
                     /locus_tag="ACHK3A_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8743053.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(846..1505)
                     /locus_tag="ACHK3A_005125"
     CDS             complement(846..1505)
                     /locus_tag="ACHK3A_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO8743054.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
BASE COUNT          463 a          368 c          375 g          541 t
ORIGIN      
        1 ctttattcgt tctcagagca tcaacatact cgtaagcctt ttcacaggtt ttattcatgg
       61 aacgaatcat acaaagtaag aaatcatctg tctctccgtt atcaccagag tttttaaata
      121 taaactcaag cattgacgtg ttttctttta tttctgctgc cacttcctca agcataatca
      181 ggggagtttt catgttcttt gttccttaaa tgcatcgcat gcgcttctgg cgtattgttg
      241 tgccagtaaa aatatgtcat ccgaaagttc atcacattct tcatcaccgg aagccgaaat
      301 gattaacccc gcttcaagca gtactgcaat gtgatgaaaa gctgtttccg gttcgttggt
      361 gaggcctttg aacattttca tcttacgatt cctttgtcgt gtcagaatgg aaacttgtaa
      421 gtaaccacat cggatcgcag tcaagtatat ttgccagagg aatcacctgg cttgccagag
      481 gttcagcctt gccgttctcc cactggatga tggtttcttc atcaactcca agcagcgtgg
      541 cgagttcagc agtggataga ccgcagcttt cgcgttgggt gcggatgcca tcacggcaat
      601 cttttttgtt tctcggaggt aacagaatat gttttagttg ttggattttt tcgattccaa
      661 cctcaaggag tacgtgacgg tttctttccg atacagactc cagactttca tgaagcagcg
      721 attctacttg ttcgataagc atcaattgtg aagtagacat aaaaaaacct cgtaaccaat
      781 atttcgaaat ctaggttacg agtgagtaat gtaggtgtca agagcaatta atgttttttg
      841 ttgaattaaa tttgcatacg catctcaacg acaacaccaa taatttcaca atccccattg
      901 atggggatca gtggatagcg tgggtttagt ggctttagga aacattgccc agcgtcttct
      961 atgtactgtt taagggttgc ctctccggag tttttgagcc tggcgacgac atattttcct
     1021 gaaaaaactt ctttgtcagg gttaacaaga atgttcatgc cttcaaaaat agagaactct
     1081 ccattctgtg atgtcattga gtcccctttc acttccagcc agaagccgtg atcgcccgca
     1141 tatatatcgc taccaatcca ttcttctgga taagctatgt aatcttcgca gttccagggg
     1201 ccagcactca cccatgttag tttcggatat ctataacggt ttggagaaac tggcttagga
     1261 tttcggacgt tttgaatgct atctgtaggt gatggggcgc tgccgtcaag aagccattta
     1321 ggagagcatt tcaatgcatc cgcgattcta aataggtttg aagatcttgt gtcttgtgag
     1381 tctcctaact ctattttgct gattgttaca cgcgagacac cggtcaattt tgccaggtca
     1441 tcttgtgaca ttcctagctg tactctgcgc attaacagtc tgccaccaaa gctatcgtga
     1501 tccatcttct cccccttctt gtcgaaataa tcgtttcgaa acaaatatag caaaaaatct
     1561 tgaaatctag gtttcggaaa attaagatta atcgaaacaa acgtgtcgag gtggatatgg
     1621 atttgtatga aattctaaaa aacatgtttg gatcgaatgt tgaaataggt cgttattttc
     1681 cccgaagggg aagggctaga accggacagg ctgtaggtaa gtggaaaacc cgaggtgtgc
     1741 cggaaga
//
LOCUS       DAZGHF010000312         1746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGHF010000312 DAZGHF010000000
VERSION     DAZGHF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.315"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..244
                     /locus_tag="ACHK3A_005126"
     CDS             <1..244
                     /locus_tag="ACHK3A_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8743055.1"
                     /translation="LHMSESLLKKKLKDEGNSFSRLLLEERMRGAVNMLCFQYGRGAI
                     VAEKCGYSSLSYFISVFHRYYGVPPDKYVSLHMVDF"
     gene            1322..>1746
                     /locus_tag="ACHK3A_005127"
     CDS             1322..>1746
                     /locus_tag="ACHK3A_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO8743056.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQ"
BASE COUNT          416 a          411 c          496 g          423 t
ORIGIN      
        1 actgcatatg agcgaaagct tgttaaagaa aaagcttaaa gatgaaggaa acagcttcag
       61 ccgcctgttg ctggaagaga ggatgcgggg ggcagtaaat atgttatgct ttcaatatgg
      121 acggggagcg atagtggcag aaaaatgcgg ttattcaagc ctgtcttact ttatatcagt
      181 attccacagg tattatggag tacctcctga caaatatgtg tctctgcata tggttgactt
      241 ttaatttcat cctgctggcg gatggagtgc tggtggtcgg cattgtggtg gtgctttttc
      301 ctttggttaa aaaggacatg ccaggtagtt agcgggtatc agtcatgccc agaaaatttt
      361 aaatgtctca caattcagac ggttgacagt tgtctgtttt gcggggagtt tgttaaaaga
      421 aactggcatg gtgaatcccc ctgagcggag gggcgactgg tgcatcaggt gttatctttg
      481 atacccagga aagtgaacgc gcgggttcgg tggcaccggg ctgaactcac cgggaggcac
      541 ccggcactat gcgcatgatg tacagatacg cggcttcagc ccctctccgg aggggctttt
      601 ctgtgcctta tgcatcacag tttctggaac cctgagtact acagtatcag tcagggtgct
      661 atatttgcag atgtgatgaa agcctgccgg caggcagggc gtatcggaaa tgacccagta
      721 agagaaaacg ttgactcaga taccggtgct gagttaccgg gaaaccggca tcacatgacc
      781 gctatccttc atggctctct caggagagcc tttttactgg agaaaaccgc ttccccgtaa
      841 aatgccgtgt tgctcacaat tcagtcagtt gaaggttgtc tgcccgactg agaatttgtt
      901 aaaaaatttc tgcatgatga atccccctgt gcggaggggc ggctggtgta cggaatggtc
      961 tctgatgatc gttagcgaga atgacgcggg ttcggtggca ccgggctgaa ctcaccggga
     1021 ggcacccggc atcatgtaat gaacggtaca tagcgctact cttcagcccc tctccggagg
     1081 ggctttctta tggataaaaa aagcccgcat ggtgagatgc gggcagcaag gaataaacaa
     1141 caaaacgtga agtaatcaat ttttcagctg gcgaataata cccgacagta atcactctgc
     1201 gcaactgcgc ggcctttttc gtattgcggg ctgtagtctt cctcctgcca ttgtcctgta
     1261 acttccggac ttcagcccgc ccctcatctg actcacaaca ttatcccgac cgggaggatt
     1321 catggcattt aaacactatg acgtggtcag ggcggcgtcg ccgtcagacc ttgcggagcg
     1381 actgactcaa aaactgaagg aggggtggca gccatttggc agtcctgtcg ccatcacgcc
     1441 ttataccctg atgcaggcca ttgcggcgga aggtgatgtc accacacctg tggtggtgaa
     1501 gccgtcggat ggagaaggca cagttatcag cgccaccagc gacccggagt attactttgt
     1561 tgtggttctg gcagggcagt caaacggcat ggcatatggt gaaggccttc cgctgccgga
     1621 gacatatgac cgtccggacc cgcgtattaa gcagctggcg cgccgcagta cggtgacacc
     1681 gggaggtgcc gcctgtaagt ataacgacat tattccggcg gaccactgtc tgcatgatgt
     1741 gcagga
//
LOCUS       DAZGHF010000313         1740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGHF010000313 DAZGHF010000000
VERSION     DAZGHF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.316"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..902)
                     /locus_tag="ACHK3A_005128"
     CDS             complement(<1..902)
                     /locus_tag="ACHK3A_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO8743057.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAAELGIPPRTVYHWCDVGQ
                     WATLLPAESVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAE
                     IAALRPAGYEDGAAAGTCGGGEGKKRRYRKNDVSGLTAEMLDEWARKNLYAYQLHCRE
                     NRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIINFAL
                     QFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMA
                     THDKYRTTYFSTPSAKTHQAYLVW"
     gene            1069..>1740
                     /locus_tag="ACHK3A_005129"
     CDS             1069..>1740
                     /locus_tag="ACHK3A_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO8743058.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     MLWPWHEEEIGHRQYTSNLGEVDSLKAETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAEIVENFARSGRDYLLGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTTHAPSKPESFLKRLFSAGQTDNTDNTDTSKPDTGEDAKMDEIKALLEKLITLVE
                     GGSAAA"
BASE COUNT          408 a          522 c          462 g          348 t
ORIGIN      
        1 ttccagacca gataagcctg gtgggttttg gcactgggtg tggaaaaata cgtcgtgcgg
       61 tatttgtcgt gggtcgccat tgccgaggcc acttcatgca gtcgcgtgaa ttttgggatc
      121 cagaacacct catcgccgta aagatggccg ttaaatccct gggcggtact ggcattggtg
      181 gacaggaaac gcagcattgc accattgctc agacggatat gcttacccgt gagcgtgatc
      241 ccgaactgca gggcgaagtt aataatatat tcacggaaga tttccgcctg tacccgtgac
      301 gcagaaaaga aaatctggtt atcacctgtc accaccgcat cttcgaacgc ctcccatgca
      361 aaataccagg tcatgccgat ctggcggctc ttgaggatga aacggcgctc acggtgccgg
      421 ttctcgcggc agtgcagctg gtaggcataa agatttttac gcgcccattc atccagcatt
      481 tcagcagtca gtccggaaac gtcgtttttc cggtagcgcc gctttttccc ttctcccccg
      541 ccgcaggtcc ccgcagccgc gccgtcctca taccctgccg gacgcagggc ggcaatttcc
      601 gccatccttt cagcgtgttt gtttttctgg gcgatgatct tcacgtgctg tgcgataagt
      661 tcacgcagtt cggcaagttc acactctgtc ttgccctccc gtccggtcag cacatcaata
      721 cgccgggcga tcacgtcctc aacggattca gcgggcagca gggtagccca ctgccccaca
      781 tcacaccagt ggtaaaccgt gcgcggcgga ataccgagtt ccgccgcaat atcgcggggg
      841 tgccaccgct tcaggtaaag cgatcgcgcc gcctctttga tttcgtcaga atatttagcc
      901 atgcgcccat tatggcggcg catccggccc gcattcaggc agaaaatttc ggctgtgttc
      961 ggctatccca cgttatccga agacagccga attcagccag tagccccccg ctgtaaaaat
     1021 cccgacactc accggagttc ttcagatttt cacgtaaaag aggccacgat gccgcagcca
     1081 acgttgatga ccgactggat atgtatcgcc acatcaggac cgaccgttga cggccgcgaa
     1141 attgatccgc agtggctgac tgatgccgct gaaacctatt cccgcaacac ctacacggcg
     1201 atgctgtggc cctggcatga ggaagaaatc ggacaccgcc agtacaccag taatctgggt
     1261 gaagtggact cactgaaagc ggaaaccggc gaggacggta aaacccgcct ctacgcgcgg
     1321 ctggtcccta accagtacct gattgaagcc aacagactgg ggcagaagct gttcacatcc
     1381 gccgagatag tggagaactt cgcccggagc ggacgcgact atctcctggg cgtggcggtg
     1441 acggatatcc ccgccagtct ggggacggaa aaaatacgct tcaccctgaa caacgaggaa
     1501 tacacagcac agcgggcgaa ctgccagacg ttcacgctgg gcagcctgtc accggacacc
     1561 acccatgccc ccagtaaacc tgaatcattc ctgaaacgcc tgttcagcgc aggacagacc
     1621 gacaacaccg acaacaccga taccagtaag cctgatacag gcgaggacgc aaaaatggac
     1681 gaaattaaag cactgcttga aaagctgatc accctggtgg aaggcggcag cgccgctgcc
//
LOCUS       DAZGHF010000314         1732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGHF010000314 DAZGHF010000000
VERSION     DAZGHF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.317"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1038)
                     /locus_tag="ACHK3A_005130"
     CDS             complement(<1..1038)
                     /locus_tag="ACHK3A_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO8743059.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILTELWK
                     ETGDPRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGVHKARQIHEEQLG
                     RTVSLRELADLLTHEGLPIHNSTISRMEDALKYLYPWIPDLMESGLGRPQVTALLALR
                     QDAERVWGQFAVAADTDAEFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALPHPS
                     LDYDRWLLELDPKERNRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPD
                     TDTEAMPESTVS"
     gene            complement(1038..>1732)
                     /locus_tag="ACHK3A_005131"
     CDS             complement(1038..>1732)
                     /locus_tag="ACHK3A_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFO8743060.1"
                     /translation="LRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDE
                     DWLRVSSAVGMALGDGGMGSLGERIIIDDCCEQTPSSLRAGARRYTRLYGKPSLIMVD
                     YLQLIRAPELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDL
                     RDSGALEQDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLF
                     TPSENDGPGGYA"
BASE COUNT          377 a          534 c          432 g          389 t
ORIGIN      
        1 ggagactgtg ctttccggca tcgcctctgt atccgtatcc ggttgacact ccggtacttc
       61 agaccggatc ccggataccg gtgccccggg ttgtaccgta ctgctgccac ggactgaagg
      121 aggcaccacc accggttccg gctcaccaaa atgatgacga cggtttcgct ctttcggatc
      181 cagttccagc aaccaccggt catagtccag ggagggatgt ggcagggcct gcaacaaatc
      241 tccgataagc tcatcgcgga acatctccag tgaccacagt tccggcgaat taaacttccg
      301 gcagctttcc ccgaacacgc ggtcaaattc cgcatccgta tcggctgcaa cagcaaactg
      361 cccccataca cgttctgcgt cctgacgaag tgcgaggagt gctgtaacct gtggacgccc
      421 cagtcctgac tccatcaggt cggggatcca ggggtacaga tacttcaggg catcttccat
      481 tctgctgatc gtggagttat gtatcggcaa cccttcatgg gttaacaaat cagccagttc
      541 acgaagagac actgttctgc ccagttgttc ctcatggatt tgtcttgcct tgtggacccc
      601 ctgcgccttc tcaataaaag tcagttcacc gcgaacttca ttttcagcca gatgaccaat
      661 cacacactgg agccgacccg gccagggttt aaacagcaca tgaatacggt aaaaacgcgg
      721 atcccctgtt tctttccaga gttccgtcag tatctggtaa cgggtgtttc ccccgtcact
      781 gaagatatac acatccggct cactgtccgg catacgggtg actttgggca ccgtatccag
      841 cccgcgggca tgaatggaat ttttgatatc gtcataacgc ggattgcgcg tggtgcgcgg
      901 gttatccgga ttcggacgta actggtccag cgtcagaacc atgggcgttt cccccattgc
      961 caccacattg ccggcaccag tggtctgacg accgggctgc ataatagccg ccccgagatc
     1021 cagtggttta tgtgccatca cgcatatcct cccggcccgt cattctcaga gggcgtaaac
     1081 agtgtgtaac gaccgtcgaa cctgacccgt accgtacccg tgggcccctg acgctgcttg
     1141 ctgacaatga tttctgcctc cccctttgcc agtgagtcgg ggttatagac ctcgtcgcgg
     1201 taaataaaca taatcaggtc agcatcctgc tccagcgccc cggaatcacg caggtcaccg
     1261 ttattggggc gtttatcagc acgctgctcc acctgccggt taagctggga gagggccagc
     1321 accgggcagc ccagctcctt ccccagcgct ttcagggaac gggaaatttc agcaatttcc
     1381 tgtgtgcggt tttccagctc cggcgcacga atcagttgca gataatccac cataatcagg
     1441 gagggtttac cgtacagacg tgtataacga cgggcgcctg cacgcagtga tgaaggcgtc
     1501 tgctcacagc agtcatcaat tattatgcgc tcacccagag agcccattcc tccgtcacca
     1561 agcgccatac cgaccgcaga tgacaccctc agccagtcct catcatccat gttgccactg
     1621 cgcaaatgat tcagttccac acgccccagc atggaaagca tgcgcagaat cagttgctcc
     1681 ttcggcatct caatgctgaa taccagaacg ctctcttccg ttcgccgcag tg
//
LOCUS       DAZGHF010000315         1731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGHF010000315 DAZGHF010000000
VERSION     DAZGHF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.318"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..902)
                     /locus_tag="ACHK3A_005132"
     CDS             complement(<1..902)
                     /locus_tag="ACHK3A_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO8743061.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAVELGIPPRTVYHWCDVGR
                     WAALLPAESVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAE
                     IAALRPAGHEDGVAAGICGGGEGKKRRYRKNDVSGLTAEMLDEWARKNLYAYQLHCRE
                     NRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIINFAL
                     QFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMA
                     THDKYRTTYFSTPSAKTHQAYLVW"
     gene            1069..>1731
                     /locus_tag="ACHK3A_005133"
     CDS             1069..>1731
                     /locus_tag="ACHK3A_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO8743062.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     LLWPWHEEEIGHRQYTSNLGEVDSLKAETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAEIVENFARSGRDYLLGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTSPAPRKPESFLKRLFSAGQTDNTDTSKPDTGEDAKMDEIKALLEKLITLVEGGS
                     AAA"
BASE COUNT          399 a          525 c          461 g          346 t
ORIGIN      
        1 ttccagacca gataagcctg gtgggttttg gcgctgggtg tcgaaaagta cgtcgtgcgg
       61 tatttgtcgt gggtcgccat tgccgaggcc acttcatgca gtcgcgtgaa ttttgggatc
      121 cagaacacct catcgccgta aaggtggccg ttaaatccct gggcggtact ggcattggtc
      181 gacaggaaac gcagcattgc accattgctc agacggatat gcttacccgt gagcgtgatc
      241 ccgaactgca gggcgaagtt aataatatat tcacggaaga tttccgcctg tacccgtgac
      301 gcagaaaaga aaatctggtt atcaccggtc accaccgcat cttcgaacgc ctcccatgca
      361 aaataccagg tcatgccgat ctggcggctc ttgaggatga aacggcgctc acggtgccgg
      421 ttctcgcggc agtgcagctg gtaggcataa agatttttac gcgcccattc atccagcatt
      481 tcagcagtca gtccggaaac gtcgtttttc cggtagcgcc gctttttccc ttctcccccg
      541 ccgcagatcc ccgcagccac gccgtcctca tgccctgccg gacgcagggc ggcaatttcc
      601 gccatccttt cagcgtgttt gtttttctgg gcgatgatct tcacgtgctg tgcgataagt
      661 tcacgcagtt cggcaagctc acactctgtc ttgccctccc gtccggtcag cacatcaata
      721 cgccgggcga tcacgtcctc aacggattca gcgggcagca gggcagccca ccgccccaca
      781 tcacaccagt ggtaaaccgt gcgcggcgga ataccgagtt ccaccgcaat atcgcggggg
      841 tgccaccgct tcaggtaaag cgatcgcgcc gcctctttga tttcatcaga atatttagcc
      901 atgcgcccat tatggcggcg catccggccc gcattcaggc agaaaatttc ggctgtgttc
      961 ggctatcccg cgttatccga agacagccga attcagccag tagccccccg ctgtaaaaat
     1021 cccgacactc accggagttc ttcagatttt cacgtaaaag aggccacgat gccgcagcca
     1081 acgttgatga ccgactggat atgtatcgcc acatcaggac cgaccgttga cggccgcgaa
     1141 attgatccgc agtggctgac tgatgccgct gaaacctatt cccgcaacac ttacacggcg
     1201 ctgctgtggc cctggcatga ggaagaaatc ggacaccgcc agtacaccag taatctgggt
     1261 gaagtggact cactgaaagc ggaaaccggc gaggacggta aaacccgcct ctacgcacgg
     1321 ctggtcccta accagtacct gattgaagcc aacagactgg ggcagaagct gttcacatcc
     1381 gccgagatag tggagaactt cgcccggagc ggacgcgact atctcctggg cgtggcggtg
     1441 acggatatcc ccgccagtct ggggacggaa aaaatccgct tcaccctgaa caacgaggaa
     1501 tacacggcac agagggcgaa ctgccagacg ttcacgctgg gcagcctgtc accggacacc
     1561 tcccctgccc cccgtaaacc tgaatcattc ctgaaacgcc tgttcagcgc aggacagacc
     1621 gacaacaccg ataccagtaa gcctgataca ggcgaggacg caaaaatgga cgaaattaaa
     1681 gcactgcttg aaaagctgat caccctggtg gaaggcggca gcgccgctgc c
//
LOCUS       DAZGHF010000316         1724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGHF010000316 DAZGHF010000000
VERSION     DAZGHF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.319"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            35..568
                     /locus_tag="ACHK3A_005134"
     CDS             35..568
                     /locus_tag="ACHK3A_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8743063.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            866..1333
                     /locus_tag="ACHK3A_005135"
     CDS             866..1333
                     /locus_tag="ACHK3A_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO8743064.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     EQANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCHGT
                     VREATSTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAKEYIRTQCIN"
     gene            1357..1581
                     /locus_tag="ACHK3A_005136"
     CDS             1357..1581
                     /locus_tag="ACHK3A_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743065.1"
                     /translation="MKKLLVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          439 a          400 c          487 g          398 t
ORIGIN      
        1 gggattcgga accgataaat cctgataaat atccatgaac accaaaatca aatacggcct
       61 gtcggctgcc gttctggcgc tgattgccgc aggtgcgcct gcgcctgaaa tcctcgacca
      121 gtttctggat gaaaaagaag gcaaccacac cacggcatac cgtgatggcg cgggtatctg
      181 gaccatctgc cggggtgcca tcatggtgga tggtaagcct gtgattcctg gcatgaagct
      241 gtcgaagggg aaatgcgacc gggttaacgc cattgagcgt gataaggcgc tggcatgggt
      301 ggagaaaaac atcaaagtgc cactgaccga accccagaaa gcggggatcg cgtcattctg
      361 tccttacaac atcggcccag gtaagtgttt tccgtcgacg ttttataaac gaattaatgc
      421 aggcgatcgc aggggagcgt gtgaagcgat tcgctggtgg attaaggacg gtggcagaga
      481 ctgccgtatc cgctcaaata attgctacgg tcaggtatcc cgtcgcgacc aggagagtgc
      541 gctggcatgc tggggtatcg acaggtagta gaatattttg ctgaaaaatg acgttgacca
      601 acgcggacgg ataacacgaa atcctgcgaa ctggcaaaac ctaagtgaat aaaagtaaaa
      661 accccgtttg ttggcagcaa gcggggtttt gtgtttcctg actccggaaa agtcaaagga
      721 gaaagtgtgt ttgattttag caaactgatt cgggagattc gagtgatggc tgaaaaatta
      781 tccacctgga agttcatcct tatctggctg gtgtttgtga ttatggcctc cggttatttc
      841 atcggtcaga tacgctggtg gtgaaatgaa ccgcgttctg tgtgtggtca tcattgcctt
      901 gctggtggcc tgtggtgcgc ttagtctggg gctgaatcat taccgcgata acgccatcac
      961 ctacaaagcg cagcgcgata aaaaagccag agagctggaa caggcgaatg ccaccattac
     1021 tgacatgcag gtgcgccagc gtgatgttgc tgcgctcgat gcaaaatact cgagggaatt
     1081 agccgatgcg agagctgaaa atgaaactct gcgtgctgat gttgccgctg gtcgtaagcg
     1141 cctgcggatc aacgccacct gccacggtac cgtgcgtgaa gccaccagca cctccggcgt
     1201 ggataatgca accggccccc gactggcaga caccgctgaa cgggattatt tcatcctcag
     1261 agaacggttg atgacaatgc agaagcagct ggaaggggcg aaggaatata tccgcactca
     1321 gtgtattaac tagtattttt gttatccgga gaatgcatga agaaattact ggtaaccgta
     1381 gaaccttttc agggaacaat tccgttccgt attttgcagc gtggtcgtgt tcttgttgaa
     1441 ggttcgttca gtggtaaatg tacgcaatta cactcccgga cctttcaggt gaatgccacg
     1501 aatgaagagc taaccgttga gtgtacgatg aatgccgcta aatgccgcat ggtatccgct
     1561 gcattacagc cagtgtgttg agcgacctta ttatccatgc gcggtattgt cgccgtattc
     1621 ccgtattaac agagaccgca gcccgacagg gagactcctc tgcgcgagtg tgcggggata
     1681 atcaaaaacg atacacaccg gggtttaccg cgttaacgga gcgc
//
LOCUS       DAZGHF010000317         1719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGHF010000317 DAZGHF010000000
VERSION     DAZGHF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.320"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..474
                     /locus_tag="ACHK3A_005137"
     CDS             <1..474
                     /locus_tag="ACHK3A_005137"
                     /inference="COORDINATES: protein motif:HMM:NF026977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743066.1"
                     /translation="PLGLLGGEQTYRYVPNPCGYVDPLGLAITSIINKTVEFSRGPQT
                     YVSDLGHVTGSTARARNKAITAILKEDFAYLKLTYIPQYNPFMRTGIAKLWEGTQFGK
                     NSFSSRAALRDVIIHEELHHRWWKRGLNDHHPKGTEMEQKFYETIRRYKRMRGWL"
     gene            474..887
                     /locus_tag="ACHK3A_005138"
     CDS             474..887
                     /locus_tag="ACHK3A_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743067.1"
                     /translation="MFGDEFKTTIECPCCGKICSIAIGQTSHNGILAWYESIICHNCG
                     LRSESDDIGFPPEKIRLQLIDFEGLWNVRLENIKSQMETIKVIRKAFSIDTKEALRII
                     RNESKVIYSGTREEAIWLMSLLKHVGETPLIQIAR"
     gene            942..1103
                     /locus_tag="ACHK3A_005139"
                     /pseudo
     CDS             942..1103
                     /locus_tag="ACHK3A_005139"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
     gene            1134..>1719
                     /locus_tag="ACHK3A_005140"
     CDS             1134..>1719
                     /locus_tag="ACHK3A_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO8743068.1"
                     /translation="MNVTTQGRRAIEYHYPDEHTVIRCILPPEDERDRHPGESLLKTT
                     YRYNAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQGWNAASQLVTQRAGF
                     FPEETTWGGLLPSLVREYRYDSAGNVSAVTSREDYGRETRREYRLDRNGQVTAVTASG
                     TGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETD"
BASE COUNT          478 a          374 c          474 g          393 t
ORIGIN      
        1 ccgctggggt tgctgggggg tgagcagact taccggtatg tgcctaatcc gtgtgggtat
       61 gttgatccgc tgggattagc tataactagt ataattaata aaacagtcga gttttctaga
      121 gggccacaga cttatgtttc tgatctcggt catgttacag gttctacggc cagagccaga
      181 aataaggcaa ttacagccat acttaaagaa gattttgcat atcttaaact aacatatatc
      241 cctcaataca atccgtttat gcgaacaggg attgctaaac tttgggaagg aactcagttt
      301 ggtaaaaata gtttttcgtc tcgtgcagca ttaagagatg ttattattca tgaagaatta
      361 catcataggt ggtggaagcg aggattgaat gatcatcatc ccaaaggtac tgagatggaa
      421 cagaaatttt acgagacgat tcgaagatat aagcggatga gagggtggtt atagtgtttg
      481 gtgatgaatt taaaacgact attgagtgtc cttgctgtgg aaaaatatgt tctatagcta
      541 tagggcagac gagtcacaat ggtatactcg catggtatga atcaattata tgccataatt
      601 gcggattacg tagtgaatca gatgatatag gctttccccc agaaaaaatt cgtctacaat
      661 tgattgattt tgagggttta tggaatgtta gacttgaaaa tataaaatca cagatggaaa
      721 ctataaaagt cattcgtaag gctttttcga ttgatacaaa agaagcactt cgaattatta
      781 gaaatgaatc gaaggtaata tactccggaa cgagagaaga ggccatctgg ttaatgagtt
      841 tattaaaaca tgtgggtgaa acccccttaa tacaaattgc tagataaatt ctatgagctg
      901 ggtacaacgg agtagcctgg cgttaccgct atgacgccga tggcctgtgt accgccaggg
      961 ttaacggtct ggaggaaacc atcctctaca gccgggacgc cgcaggccgc ctggcagaag
     1021 tcatcacgcc ggaaggcaaa acgcagtatg cgtatgacaa atccggcagg ctgacgggta
     1081 tcttcagccc ggacggcaca tcacagcgca ccggctatga cgaacgcggg cgggtgaatg
     1141 tcaccactca gggccgacgg gccattgaat accactaccc cgatgaacac accgtcatcc
     1201 gctgtatcct gccaccggaa gatgaacgcg acagacatcc cggtgaatcc ctgctgaaaa
     1261 ccacctaccg ctacaacgcc gccggagaac tgacggaggt tatcctgccg ggggatgaga
     1321 cgctgacgtt cagccgtgat gaggcaggac gtgaagtgct ccggcacagt aaccggggtt
     1381 ttgcctgtga gcagggctgg aatgcagcca gccagcttgt cacccagcgc gccggatttt
     1441 tcccggagga aaccacatgg ggcgggctgc tcccctcact ggtacgggag taccgttacg
     1501 acagcgcggg caatgtgtcg gctgtcacca gccgggaaga ttacggacgg gaaacacggc
     1561 gggaataccg gctggaccgg aacggtcagg tcacggcggt gacagcctca ggcaccgggc
     1621 tgggctatgg cgaaggcgat gagtcctatg gctatgacag ttgtggctac ctgaaggcgc
     1681 agtctgcggg caggcaccgg ataagtgaag agactgacc
//
LOCUS       DAZGHF010000318         1709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGHF010000318 DAZGHF010000000
VERSION     DAZGHF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.321"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            50..781
                     /gene="bluR"
                     /locus_tag="ACHK3A_005141"
     CDS             50..781
                     /gene="bluR"
                     /locus_tag="ACHK3A_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO8743069.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            1002..1406
                     /locus_tag="ACHK3A_005142"
     CDS             1002..1406
                     /locus_tag="ACHK3A_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO8743070.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRVAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTKKNPAKIKEAALLHISGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            1459..1569
                     /locus_tag="ACHK3A_005143"
     CDS             1459..1569
                     /locus_tag="ACHK3A_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743071.1"
                     /translation="MEIKVQRLSLWMINTVFLLPPINNHQANTINLIFEM"
BASE COUNT          511 a          363 c          367 g          468 t
ORIGIN      
        1 tattgggtcg tgtacaggcg acggagattt gtgaccgcaa ggaggaattg tggcttatta
       61 cagcattggt gatgttgctg aacgttgcgg gattaatcct gtcactctcc gggcctggca
      121 acgccgctac ggtttgttaa aaccacagcg cagtgaaggc ggacaccgac tctttgatga
      181 agaagacata caacgcatcg aagagatcaa gcgttggata agtaatggcg ttcctgtagg
      241 caaagttaaa gcattactgg aaaccaccag ccaggatacg gaagatgact ggagtcgcct
      301 gcaagaagag atgatgtcaa ttcttcgtat ggctaatcct gccaaactac gcgcgagaat
      361 tatttcactg ggtcgagagt acccagtaga tcaattgatt aatcatgttt accttcctgt
      421 tcgccagcgt ctcgtgcttg atcacaacac ctcccgcatt atgagcagta tgtttgacgg
      481 cgcattaatt gaatacgcag cagcctcact tttcgaaatg cgccgtaagc ccggtaaaga
      541 agccattctg atggcgtgga atgttgaaga gagagcacga ttgtggctgg aagcatggcg
      601 tttatcattg tcaggatggc acatttctgt tcttgctgat cccattgaat cgccgcgccc
      661 ggaactgttc ccgacgcaaa cattgattgt ctggacaggc atggcaccaa cgagaaggca
      721 aaacgaactt ttacaacact ggggtgagca aggttataaa gtcatctttc atgcccccta
      781 atttcacctt tatcccgccc cttagacaga atcttataat ctgaacccac aacagctttt
      841 gtaccggagc tggtccgttg caaaatgctc accgggagaa ttcaggtaat ccccctttca
      901 cactttgtac atatcacagc caaaggacag accagattga agtggtaaaa ccgatcgtgc
      961 acactacgca gtctcacaac taacaagagg ttattatcct gatggatcag gtcgttgttt
     1021 ttcaaaagat gtttgagcaa gtgagaaaag agcaaaactt ctcctggttt tattcagaat
     1081 taaaacatca ccgcgttgca cattacattt attatctggc tacggataac atcagaatta
     1141 ttactcacga tgacacggtt ttgttattaa gaggaaccag gaacctgtta aaagttagta
     1201 cgaaaaagaa ccctgctaaa ataaaagagg ccgcattgct tcatatttcc ggaaaatcta
     1261 catttcggga gtactgttca acactggcag acgcgggcgt tttccggtgg gttactgatg
     1321 ttaatcataa caaacgcagt tactacgcca ttgataatac gcttttatat attgaagatg
     1381 tagaaaataa taaaccatta atctagctaa agttggatgc ttaaaaaatg cttcataatt
     1441 cagtaagaca ttagcataat ggaaataaaa gtgcagagac tatccctatg gatgataaat
     1501 actgtctttt tattgccacc cataaataat caccaggcca atactatcaa cttgatattt
     1561 gaaatgtgat cacttgactt tctatacgtt attttataac ggttaacata tttataaaac
     1621 caacgggcgt gccatacgcc cgtttcaata cttaacgcac atgtgttttg gtttagtcat
     1681 catccgatta tatgtatttt agtcaggaa
//
LOCUS       DAZGHF010000319         1692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGHF010000319 DAZGHF010000000
VERSION     DAZGHF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.322"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            4..738
                     /gene="nanR"
                     /locus_tag="ACHK3A_005144"
     CDS             4..738
                     /gene="nanR"
                     /locus_tag="ACHK3A_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO8743072.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(774..>1692)
                     /locus_tag="ACHK3A_005145"
     CDS             complement(774..>1692)
                     /locus_tag="ACHK3A_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8743073.1"
                     /translation="NDKSLNEMMLSLFNKSTIVDLSSETISESKSLSGRIYQDPEHNG
                     EFELDRVLTDIYAPQLIIFLPDKPNGTGILIIPGGGYRAVMLDKEGTALAPEFLSQGY
                     TLFVLSYRLPSPAEEENINKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFSAGGHLA
                     STFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPDSRKQLLGDNSTSE
                     NIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGH
                     GFSVQWATDLPVGGWIKLFMDWMYSHGFK"
BASE COUNT          584 a          350 c          296 g          462 t
ORIGIN      
        1 tatatgtccg acaacaaaaa aaccgtgtta cctgggctaa caacgaccag aaagcccatt
       61 gttacaaaaa aactttcaga aatcgttgcc gaagagcttg aaagaatgat ccgccaggag
      121 gagataaggg aaggtgaata cctgccttct gaacgtgaac ttatggcatt ctttggcgtt
      181 ggaagaccat ctgtcagaga ggctttatca tctctcagac ataaagggct aatccggtat
      241 aacaagggag aaaaagccag tgtctgccgt ccatcaccag aaaccatcat taatgggtta
      301 tcaggcattg ttaaagattt tttatccaca gctgatggca tcaaggattt tgaaagacta
      361 cgcatctttt ttgaaaccaa ccttgtaaga tatgcagcaa aaaatgcaac gcctgaggat
      421 attcacaggc ttgagaaggt gctcagtatc catcataact cactgagaac ccccgaagtg
      481 tatgctcaat atgatattga atttcatagt atacttgctg atatacctga caataaagta
      541 ttaaaaacaa tacactcagc attttctgaa tggctgataa tggccaaacc tctggaaaag
      601 aataaaatga catatgaaaa taacaaaaaa acccatcagc agcatattga aatacttacg
      661 gccataaaag agcatgattg tgataaagct gaacagctaa tgtataatca cctttataaa
      721 cgaattgaag catcatagtt actcgacgct tcaccaggta gtcaccataa atattattta
      781 aatccatgag aatacatcca gtccataaac agttttatcc atcctccaac aggaagatct
      841 gtggcccact gaacactaaa gccatgccct cctttctcat agagatgcat ttcagcagaa
      901 acgcctttct ctattaggcc acggtacatg agcagactgt tttcaattga cacaagatca
      961 tcatcacaac aatggagtaa aaacacagga ggaaaacatg atgtgacatt aagatccgca
     1021 ctatattgta ttatattttc tgaggtacta ttgtccccca gtaattgctt cctggagtct
     1081 ggatgtgtca catcatcttt cattgatatt acaggataaa ttaacccgac aaaatcggga
     1141 atagcgaaaa tctcttgttc tttactgtca gaagaacgtt tgaaatcaca aatctgattc
     1201 acaaacgttg aggcgagatg ccccccagca gaaaatccta atacaccaat acgatgacgc
     1261 tgtagatgcc atttatcagc attcttacga atataaagca ttgcagatat tgcatcaata
     1321 aaagttttgt ttatattttc ttcctcagct ggcgatggta atctgtaact aagaacaaat
     1381 aatgtatagc cctgagataa aaactctgga gcaagagctg taccttcctt atctaacata
     1441 acagcccggt agcccccccc gggaataatc aatattcctg ttccatttgg tttatctggt
     1501 aaaaaaataa ttaactgagg agcataaata tctgtcaata cacgatcaag ttcaaattcc
     1561 ccgttatgct caggatcctg ataaatacgg cctgaaagag atttactctc agatattgtc
     1621 tcacttgaca agtcaacaat agttgattta ttaaacaagc ttaacatcat ttcatttaaa
     1681 gatttatcat ta
//
LOCUS       DAZGHF010000320         1690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGHF010000320 DAZGHF010000000
VERSION     DAZGHF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.323"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..352
                     /locus_tag="ACHK3A_005146"
     CDS             <1..352
                     /locus_tag="ACHK3A_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO8743074.1"
                     /translation="SLTLKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRL
                     NAGIAGVTAISSIPYVAENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDS
                     SHNTAIGAGVAGGW"
     gene            complement(1278..1520)
                     /locus_tag="ACHK3A_005147"
     CDS             complement(1278..1520)
                     /locus_tag="ACHK3A_005147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743075.1"
                     /translation="MMMARDITAYHLNSVHISARRFPGIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
BASE COUNT          520 a          405 c          334 g          431 t
ORIGIN      
        1 ttcactgaca ctgaaacagg caacagccta cagcgatatc agtacccgcc gggcactgtc
       61 agaggccaat cgttataccg acaacaaatt cagaaacctg agcaataaaa ttgaacgggc
      121 agaaaaaaga cttaatgcag gcatcgccgg agtgacagca atatcctcca tcccctatgt
      181 ggctgaaaat tctttttcat acgggatcgg cgtgggtaac tatcagaacg ctaatgctgt
      241 ggccgcaggc attcagtata aaacatcacg taataccaat atacgcctca acatctcctg
      301 ggactcatct cataacaccg cgattggtgc aggggttgcc ggcggctggt aaataatctt
      361 gcgtacatac ggcattatgg tattccgtaa tcacattaat tcagtacgca acacagcccc
      421 atccggaaca gaccactgga ctgagcggga taatcaagga aaaagggcgc gcagtgtggt
      481 cagccttcac aacaatccgg cggcagattt cctgccgcca tgttgcccgt aaacatttaa
      541 gttcgcaggc taacattatt gccacaagcc ccgtatttcc ctggtgaatc ctcaaagcga
      601 gtccctcacc atccagacaa aacacagtat cgtcgattaa taatcactaa aagcgttcgt
      661 cgtagcatca taccccctga atgaacacat ccctgtttgc aaggattctg ttgttctgat
      721 atcgacgagt agctgcaact ctgtatccgc acttattgca atcagtgcag tctggccacg
      781 atgcgattcg ctgacctgag ccttattctc caggcggact ttatgcaatg ctcccctgaa
      841 atcttccatt attttacatg tacaggcatc atattaaata tggaaataat aacgaacgga
      901 aaagggacac aatgaatatc aaagcaccag tataaaccag gatgttttga gcaatttctt
      961 actctcctga atacacactg taaatcatta gaaactgcac tagccatgcc aagtacactc
     1021 tgctacgaac tgatgaggaa gtgcggtatc aattacctgg acagcctgat caacggagag
     1081 gtatatctat tatctatgta attacagggg gactgatatc agcgaaaaag caggtcagca
     1141 tattcactgc ccctggtctg aactgaaaca acgtcagcca gaaataatcc tataatgcct
     1201 catataagtg gataactttt catccgtcgt tgacatcata cacataaaac attgataaca
     1261 gtcatgaata caccgaatta tctttctgac tcagcgtttc cagtcagtag catttcttcc
     1321 ggtaaaggca tttccgaaac gtcacgttcg ccattaaata caaaaatact ttctgcgtaa
     1381 tcattctgta acaaagaatt agttaacggc agattttttg tttgcatacc ggtggcccac
     1441 tgaaaaatcc cggggaaacg acgggcactg atgtgtacag aattgaggtg gtacgcagta
     1501 atatctcttg ccatcatcat aagatggcac atcatatccc gaacgcaaac ctggcacatg
     1561 atgaacagac ggaaggctca gcaggacttc tggataatta tacacgatag ccagttactg
     1621 ctgacctgga tttattatca taccacatta tttatttcaa aaaataaatt ttgaattact
     1681 tgtttttact
//
LOCUS       DAZGHF010000321         1642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGHF010000321 DAZGHF010000000
VERSION     DAZGHF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.324"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..546
                     /locus_tag="ACHK3A_005148"
     CDS             <1..546
                     /locus_tag="ACHK3A_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO8743076.1"
                     /translation="IRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLSSTRNTMINA
                     GQHLDIGAMKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVT
                     SSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGANLPNEA
                     PGFSLTDITQESKISSKSFND"
     gene            562..>1642
                     /locus_tag="ACHK3A_005149"
     CDS             562..>1642
                     /locus_tag="ACHK3A_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743077.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPLPFKGEQAIRCMADGEVIAYRICRDYLT
                     LGWESGPLSFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYESEAENQWVVQD
                     TLKAYSEMDWLTAKLTSDETSPQIAGYMPEGARVEWDPADSNLNATGSNKRKYGLVTL
                     KGLPEKTSSTLTPGQRYWVVVDNNNVKPAAGAGPGWWRHLLPPAKEAMVFDKTVSLRS
                     PFAIKAGDPIGHMGYYQAPKDGGYEARYQVHIECTSMDDNLEKFLTNPEQVGKNNPLW
                     LKYAPGLALYKKDVSTGTFTKDTGVT"
BASE COUNT          446 a          374 c          455 g          367 t
ORIGIN      
        1 attaggtttg taaccgataa aatcagggaa cttcagtctg cggtcctgct gggtagcgcg
       61 ccacaggggg tggccctgac ctcaggtgaa caccttcagc tcagcagtac ccgtaatacc
      121 atgatcaatg caggccagca tcttgatatc ggtgcgatga aaaatctgtc ggtaacggtg
      181 gaaaaagccc ttggtctgtt tgtgcataaa gacggggcga agctggtagc caatcaggga
      241 gatattgaga ttcaggctca gcacaacacg atggcgctgt tttcagaaaa acagctgacg
      301 gtgaccagta gtgaagacga aattattatc agcaccccgg aaaccctgac gctgaacggt
      361 ggcgggtctt acctgagact gagtaagaac gggatagagc acggctcatc gggtgagttt
      421 attgtgaaga cgtctgacta tctggtgcct ggttctggcg caaatctccc caacgaagca
      481 cctggtttca gcctgacgga tatcacacag gaaagcaaaa tcagcagcaa gtcttttaac
      541 gactgacagg gagtatgaat cgtgtctgta ttacccaaaa tcgtctggcc gatcccctcc
      601 agccgtcggg gaagtgagtt caaaaatcag gaagagatcc tctctcattt gggaggagaa
      661 tctaccgggc tgtacatgat aggccgtagt ggcatgtggc atggtgggat ccatatcacc
      721 gaagccacga caccatggtg tgcgcttagc ggtaagtctc cacttgaagc gatagatttt
      781 ccgcttcctt ttaaaggtga gcaggcaatc cgatgcatgg ctgacggaga agtgattgct
      841 taccgcatct gcagggatta tctgacgcta ggctgggaat caggtccgct aagtttttcg
      901 ggctcatttg ttctggtgaa acactatatc cagccgggtg agaaagaaac cagcggactg
      961 catttctata ctctgtatat gcatcttgcg ccgtattcag cttatgaatc tgaagcagaa
     1021 aatcagtggg tcgttcagga cactctaaaa gcatattctg aaatggactg gctgacggcg
     1081 aaactgactt cagatgaaac ttctccacaa attgcagggt acatgcctga aggggccaga
     1141 gtggagtggg acccggcaga cagcaatctt aacgctacag gaagtaacaa gagaaaatac
     1201 ggtcttgtga cgctaaaggg tttaccagaa aaaaccagca gtactctcac tcccggtcag
     1261 cgctactggg ttgttgtgga taacaacaat gttaagccag cggcgggggc cgggcccggc
     1321 tggtggcgac acttgctgcc tcccgcaaag gaagccatgg tattcgataa aacagtaagc
     1381 ctccgttccc cattcgccat taaggctggt gacccgattg ggcacatggg ctactatcag
     1441 gcaccaaagg atggggggta tgaggcacga tatcaggtac atatcgaatg cacgagtatg
     1501 gatgataatc tggagaaatt cctgaccaat cctgagcagg tgggaaaaaa taatccactc
     1561 tggctgaagt acgccccggg acttgcgttg tacaagaaag atgtttctac aggaacattc
     1621 acgaaggata cgggggtgac gg
//
LOCUS       DAZGHF010000322         1642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGHF010000322 DAZGHF010000000
VERSION     DAZGHF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.325"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..546
                     /locus_tag="ACHK3A_005150"
     CDS             <1..546
                     /locus_tag="ACHK3A_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO8743078.1"
                     /translation="IRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLCSTRNTMINA
                     GQHLDIGAMKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVT
                     SSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGVDLPNEA
                     PGFSLTDITQESKISSKSFND"
     gene            562..>1642
                     /locus_tag="ACHK3A_005151"
     CDS             562..>1642
                     /locus_tag="ACHK3A_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743079.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPVPFKGEQAIRCMADGEVIAYRICRDYLT
                     IAWESGPLNFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYESEAENQWVVQD
                     TLKAYSEMDWLTAKLTSDETSPQIAGYMPEGARVEWNPADSNLNATGSNKRKYGLVTL
                     KGLPEKNSSTLTPGQRYWVVVDNNNVKPAPGAGPGWWRHLLPPAKEAMVFDKTVSLSS
                     PFAIKVGDPIGHMGYYQAPKDGGYEARYQVHIECTSMDDNLEKFLTNPEQVGKNNPLW
                     LKYAPGLALYKKDAFTGTFTKDARVT"
BASE COUNT          443 a          371 c          455 g          373 t
ORIGIN      
        1 attaggtttg tgaccgacaa aatcagggaa cttcagtctg cggtcctgct gggtagtgcg
       61 ccgcaggggg tggccctgac ctcaggtgaa caccttcagc tctgcagtac ccgtaacacc
      121 atgatcaatg caggccagca tcttgatatc ggtgcgatga aaaatctgtc ggtaacggtg
      181 gaaaaagccc ttggtctgtt tgtgcataaa gacggggcga agctggtagc caaccaggga
      241 gatattgaga ttcaggctca gcacaacacg atggcgctgt tctctgaaaa acagctgacg
      301 gtgaccagta gtgaagacga aattattatc agcaccccgg aaactctgac gctgaacggt
      361 ggtgggtctt acctgagact gagtaagaac gggatagagc acggctcatc gggtgagttt
      421 attgtgaaga cgtctgacta tctggtgcct ggttctggcg tagatctccc caacgaagca
      481 cctggtttca gcctgacgga tatcacacag gaaagcaaaa tcagcagcaa gtcttttaac
      541 gactgacagg gagtatgaat cgtgtctgta ttacccaaaa tcgtctggcc gatcccctcc
      601 agccgtcggg gaagtgagtt caaaaatcag gaagagatcc tctctcattt ggggggagaa
      661 tctaccgggc tgtacatgat aggtcgtagt ggcatgtggc atggtgggat ccatatcacc
      721 gaagccacga caccatggtg tgcgcttagc ggtaagtctc cacttgaagc gatagatttt
      781 ccggttcctt ttaaaggtga gcaggcaatc cgatgcatgg ctgacggaga agtgattgct
      841 taccgcatct gcagggatta tctgacgatt gcatgggaat ccggtccgct gaatttttcc
      901 ggctcatttg ttctggtgaa acactatatc cagccgggtg agaaagaaac cagtggactg
      961 catttctata ctctgtatat gcatcttgcg ccgtattcag cttatgaatc tgaagcagaa
     1021 aatcagtggg tcgttcagga cactctaaaa gcatattctg aaatggactg gctgacggcg
     1081 aaactgactt cagatgaaac ttctccacaa attgcagggt acatgcctga aggggccaga
     1141 gtggagtgga acccggcaga cagcaatctt aacgctacag gaagtaacaa gagaaaatac
     1201 ggtcttgtga cgctaaaggg tttaccagaa aaaaacagca gtactctcac tcccggtcag
     1261 cgctactggg ttgttgtgga taacaacaat gttaagccag cgccgggggc cgggcccggc
     1321 tggtggcgac acttgctgcc tcccgcaaag gaagccatgg tattcgataa aacagtaagc
     1381 ctcagttctc catttgccat taaggtcggt gacccgattg ggcacatggg ctactatcag
     1441 gccccaaagg atggggggta tgaggcacga tatcaggtac atatcgaatg cacgagtatg
     1501 gatgataatc tggagaaatt cctgaccaat cctgagcagg tgggaaaaaa taatccactc
     1561 tggctgaagt acgccccggg gcttgcgttg tacaagaaag atgcttttac aggaacattc
     1621 acgaaggacg cgagagtgac ga
//
LOCUS       DAZGHF010000323         1634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGHF010000323 DAZGHF010000000
VERSION     DAZGHF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.326"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            193..375
                     /locus_tag="ACHK3A_005152"
     CDS             193..375
                     /locus_tag="ACHK3A_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8743080.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            401..646
                     /locus_tag="ACHK3A_005153"
     CDS             401..646
                     /locus_tag="ACHK3A_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO8743081.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASDGGDPTPDSDMMM"
     gene            723..938
                     /locus_tag="ACHK3A_005154"
     CDS             723..938
                     /locus_tag="ACHK3A_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8743082.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            942..1169
                     /locus_tag="ACHK3A_005155"
                     /pseudo
     CDS             942..1169
                     /locus_tag="ACHK3A_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
BASE COUNT          460 a          334 c          457 g          383 t
ORIGIN      
        1 tgctgattga cagtgtgaag gtggaagtga acaacgcgga cgccgcggca tgataaaaag
       61 agccgccagc ggcaggaatg gaagctggcg gaggtaatcc caatggagaa tgtaaagaaa
      121 agatgctttc gtacatcggt tgttttttta aatgaaaaca gttctcattg tcaaccataa
      181 cggtaagaaa ttatgacatt tattcatcag gtgatgctgt acttctgtac ggcggtctgt
      241 gtgctgtatc ttctttcggg tgggtacagg gcagtgcgcg atttctggcg caggcagatt
      301 gataaaaggg ccgcagagaa aatcagcgcc agtcagtcag ccggaagcaa acccgaagag
      361 cccgttactc cttaacaacc cctttcagcg agaaaatccc atgtcagaaa tcacatccct
      421 ggtcactgct gaagcagtga aggaagtcct gcgctctgaa gaagtcctga gcgcactgaa
      481 acagaaactc cgccagaacc ttgaggcgcg tcttgatgca gaagtggatg ccattctgga
      541 tgaactgctg ggcgcaccgg cagttccgga gccggaaggt atcgcgggtg aggggagtgc
      601 ttcagatggc ggtgacccca cacctgacag cgacatgatg atgtaagcat gcgcaaggga
      661 ccatcggtgt gtgccggtgg tctttatatt gttgtgagct tccggattgc gggagacggg
      721 gtatgtacca gatggaaaaa atcacaacag gtgtgtcata caccacgtca gcggtgggaa
      781 cgggctactg gttcctgcag ttgctggaca gggtttcccc gtctcagtgg gcggcaatag
      841 gtgtgctggg gagtctgctg tttggtctgc tgacatatct gacgaacctg tatttcaaaa
      901 tcagagagga ccgtcgtaag gcggcgcggg gagagtagac gatgaataaa aaatatgaac
      961 tggttgtaaa agggataaat cattactcgg ataagataac tgttactgtg acactggaaa
     1021 ttgccgggca gccgtcgttg ttgtcgccat atgtggcgat tagtcttgac cgtactgaag
     1081 gtgccacgct ggaattttac gaagctgagg cgaaaaaaca ggcgaagcag tttttcatgg
     1141 atattgctgc cgctttatgt gaaggggatg aacagtcaca ggaaaagcgc ctctgttcag
     1201 aagagcggta taccatacaa attaacaatg catataacac gatacagtca gaaaaagatg
     1261 atatcgaatc acgcattgaa aaactggaga acagcgtcgt tgaactgaat aaaaaactgt
     1321 cggtgcagat cccttccgaa ggtgaaaaaa aacgccgtga tgagcagttc gcggcgtttt
     1381 acgattattg ccggaaagtg atgagcagaa atctcgcaga gtgtttccgt attcaaaaaa
     1441 acagagagcg ctctgcaaat agtgagattc tggataattt tttaaagggg acgcatctcg
     1501 ccagtgattg tttcattgct cattcttttg gtggctttca tgaggccatc aaacatgatg
     1561 ttgagagaag tccaggtatc ctctgttcca ttcccgtaag catcgaaata gacacaaata
     1621 cgataaccgg tgaa
//
LOCUS       DAZGHF010000324         1631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGHF010000324 DAZGHF010000000
VERSION     DAZGHF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.327"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1060
                     /locus_tag="ACHK3A_005156"
     CDS             <1..1060
                     /locus_tag="ACHK3A_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO8743083.1"
                     /translation="MGPKGEKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPR
                     GEPGPAGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETKA
                     ATPKAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEF
                     VAWLKEKGAFDQPYWMMKASLLAEFNKVITDVGPGKLNLGGCAIEVMGTYNAAIVRVT
                     IGEYGGDGFLNGTVCTCTVYGDTQRFHWRVDYSTKNKPTTASLTVNGWERDEVTGRLR
                     QWGSIEVSEDDGKLMTQHSIRFPVAFPVAALNAQVSAVGDPHAIKMYTLSNPSLVSVT
                     LITVRGCYGKFSWEAIGY"
     gene            1070..1294
                     /locus_tag="ACHK3A_005157"
     CDS             1070..1294
                     /locus_tag="ACHK3A_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743084.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            1291..>1631
                     /locus_tag="ACHK3A_005158"
     CDS             1291..>1631
                     /locus_tag="ACHK3A_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743085.1"
                     /translation="MMHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLK
                     VVYDSRGVITGYGKDTALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAE
                     KARGMKGDEINA"
BASE COUNT          407 a          347 c          558 g          319 t
ORIGIN      
        1 tatggggcca aaaggcgaga aaggtgatcc gggaggtcct gcaggcccgc aaggtcctaa
       61 aggtgaacga ggagaagccg gaccacaggg accgatggga gcacgaggtg agcgtgggga
      121 gactggcccc cgaggtgaac ctggtcctgc aggtccgaga ggcgaacgag gagagaccgg
      181 acctcaggga cctcgtggag agccaggtcc ggcaggcagc gctgcaaatg tggctgatgc
      241 gacgacggca cagaagggaa ttgtgcagtt aagcagcgca acggacagtg atgatgaaac
      301 gaaggcagcc accccgaaag cggtgaaagc ggcaatggac aaagcggacg gatgcctgga
      361 gaaagcgaaa aacggtgacg atatcccgga taaggtgaag tttctgaaca ccgtgggggc
      421 agccagagta tacgggcggg acattcatac gggggccggt gaatggacca cgagcgagtt
      481 tgtggcctgg ctgaaagaaa agggggcatt tgaccagcct tactggatga tgaaggcatc
      541 actgcttgcg gaatttaata aggtcatcac ggatgtcgga ccgggaaaac tcaatctggg
      601 aggctgcgcc attgaggtga tggggacgta taacgcagcc atagtccggg tcaccatcgg
      661 cgaatacggt ggcgatggtt ttctgaacgg cacggtctgt acctgtacag tttacggaga
      721 cacacaacgt tttcactggc gggtggatta cagcacaaaa aacaagccca caacagcaag
      781 cctgacagta aatggctggg agcgtgatga agtaaccggt cgtttgcggc aatggggcag
      841 tattgaggta agtgaggatg atggtaagct tatgacacag cactctatcc gttttcctgt
      901 cgcttttccg gttgctgctc tgaatgctca ggtgtctgca gtgggagacc cccacgccat
      961 taagatgtat accctgtcga atccgtcatt agtgtctgta actctgataa cagtcagggg
     1021 atgctacggc aagttttcat gggaagcgat cggatactga gggcattgta tggaacagta
     1081 tgttttttca ccgtcagaga atatgttcta tcccctgtcc ctgcggccgg tttatgaggc
     1141 agcagggcgc tggccggaag acggtattgt ggtggattat gtggtgtata aagtctttgc
     1201 agcggatgct gcaccggcag gtatgaagcg gggcgtcggt gcggaaaaaa tgccggtctg
     1261 ggtgccggta tcggaggagg gcacggaaac atgatgcata tcagaaattt ttcgtattac
     1321 accccggcgg agccggatgt tgcaggcgcg atgtacctga agtctgagga tggtcaggac
     1381 tggtatgagt gtcaggcgtt gttttcgccg gagacgctga aggtggtgta tgacagccgg
     1441 ggtgtgatta cgggatacgg taaggacact gcgttactgt ggccggtgaa ccagagtgtg
     1501 gcagaagtgc cggatacccc ggagagccgg aaaattgacc tgagcgggcg ctggggtttt
     1561 gacggggaga aaatcacgga cctgctgacc gcggagaaag cgcgagggat gaagggcgat
     1621 gaaataaacg c
//
LOCUS       DAZGHF010000325         1624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGHF010000325 DAZGHF010000000
VERSION     DAZGHF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.328"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..282
                     /locus_tag="ACHK3A_005159"
     CDS             <1..282
                     /locus_tag="ACHK3A_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HFO8743086.1"
                     /translation="LTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVPAFTFAQDA
                     PVVVRAPGYRAGRTMYWLSWVAGIVTLAGLWCVLSSVLADQVARLTGQG"
     gene            285..>1624
                     /locus_tag="ACHK3A_005160"
     CDS             285..>1624
                     /locus_tag="ACHK3A_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8743087.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWYCLSSRRDAPEKAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQ
                     QRGVPQRYGLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPKMVRLNSLA"
BASE COUNT          306 a          426 c          494 g          398 t
ORIGIN      
        1 ctgacatgcc tctgccgtac gcttcagctc ggttttaccg gtcagtaccg gtcgcaggat
       61 gatgagcgtc gcgaagatgt aattcgcgca ctgacagcgc gtgttcctgc atttactttc
      121 gcacaggatg cgccggtggt ggtgcgtgca ccgggttacc gtgctggcag aacgatgtac
      181 tggttgtcat gggtggcggg tattgtgacg ctggctggtc tctggtgtgt gctttcttct
      241 gtgctcgcag accaggtggc acggcttacc gggcagggat aaccatgcac gtacgttccc
      301 gtctctggct gacaatactg agtctgttcc tgacactgtg gctgatttcg ggattcctgc
      361 cactgtctgc agtaaaccag ataatactga ccctggtttc agttgtggcg gcgggactgg
      421 tgctgtggta ccagtggtat tgtctgtctt cccgtcgtga tgccccggag aaggctggcg
      481 atgacggtct gccgcctgaa tattttcagg gacatgtgct gcttgttgcc ggaaactcag
      541 atgcgtggtt cgaacccggt caggcatacc gggaaacagc atcgggatgg tatcttcgtg
      601 tggatacgcc ggagcagatg agaatactgg cagagcggtt gtcgtacagc cgtccggccc
      661 tgtgtggcca ggtcgtggtc atgctgggac ttttaccgga gcagcatacc tcagatgaag
      721 ctttcctgtc gtggttgcgg aactggcagc gtgcagtcat gcagtgccgg cagtggctga
      781 ataccatgcc gccggtgctg gtgacattga tggtttcagg gccgtcacca caaaacacat
      841 ccttaaataa gcactctgca cactggttta cactgacacc ggacctgtcg ggaatgcaca
      901 tacgcctgac ggaagccgga gccattccgg cagaggaatg gctccgggag tgtgatgcag
      961 cgtccaggct ccggtgcgtc agtgaactgt tatggctgaa tgccatgttg tcctggtaca
     1021 accgggttac tgatgccctg acagatattt gccggcagga gcacttttat ttccggccgg
     1081 ttgccgttgg attctgtctg acgtcggttg cggcccggcc cggtaatctc tggcaacaac
     1141 aactgacgtc actcactgta ctgccaccgc ctgccggcgt gtcatcacag gcactgccgt
     1201 tgccggatat tctgctgtcc ggtattcccc gtcatcgtgg actcagtcac tggcacagac
     1261 tttgtctcca gacgggggcg attgtcggcg tttttcttct gctggctatg ctggcgtctt
     1321 ttatgaataa ccagcgactg gttcgcagcg tgggggaaca cctgacggct tggcaccgga
     1381 tgcgcggata ttcatctgaa tcgaaaaaaa ctgcactgca acgtcttcag gctgacagcc
     1441 agttactgaa cgactggcag caacgtggtg tgccgcagcg ttacggactg ggattgtatc
     1501 agggaatgtg gctgataccg ccggttgagc gagcgactgg tgagtggatt gcaccactgc
     1561 ctccccgtcc ggtaatacag aaagttactc agacgccgaa aatggttcgc cttaacagcc
     1621 tggc
//
LOCUS       DAZGHF010000326         1615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGHF010000326 DAZGHF010000000
VERSION     DAZGHF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.329"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(330..557)
                     /locus_tag="ACHK3A_005161"
     CDS             complement(330..557)
                     /locus_tag="ACHK3A_005161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743088.1"
                     /translation="MSHLIKRYTNYDTTLLADILSCIAANIEDAYILSGAVAGRDYNY
                     NDLMSLAVKYLSGTKQIPDIHTETIIRGEPD"
     gene            complement(738..932)
                     /locus_tag="ACHK3A_005162"
     CDS             complement(738..932)
                     /locus_tag="ACHK3A_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743089.1"
                     /translation="MNITRNSKLKEHTMNDIRRISDRELTKMIESAQQLLSRQNPVVE
                     ETWWENLLAALTELRERRQQ"
     gene            complement(941..1345)
                     /locus_tag="ACHK3A_005163"
     CDS             complement(941..1345)
                     /locus_tag="ACHK3A_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743090.1"
                     /translation="MTRFYCAMQRQRFDEMMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTAIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLASINRKAAIARYVHSAANRQSRR"
     gene            complement(1342..>1615)
                     /locus_tag="ACHK3A_005164"
     CDS             complement(1342..>1615)
                     /locus_tag="ACHK3A_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8743091.1"
                     /translation="PPPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITRELDALG
                     VQLHESAVVSVRYGARVRLADGRTAYWNDSTRRIDITPAPNTEAPQ"
BASE COUNT          304 a          388 c          475 g          448 t
ORIGIN      
        1 ctgtgcatgc agtgatgtcg aatgcgggca taccttcgtc tgtacgatga ccttttccca
       61 taccctcagc cccggtgccc gctccggtcg caatatggtg aagtttcttg ttgatgccat
      121 gcgcccggaa gatcgccggt atgcgcttga actactgtcg cggcgttcct gacgcgcttc
      181 gcttgtctgc tgcttcgccg gggcaaaaag aatttttacc ccggcgaagc agccctgata
      241 aagggggggg tggtgtaaat tcggacgtta taaaattaaa aaagcgccgt aacggcgctc
      301 gggtagagta tttattcatg caaaaatagt taatccggct cccctcgtat tattgtttct
      361 gtatgaatat cgggaatctg ttttgtaccg gatagatatt ttacagcaag actcattaag
      421 tcattgtaat tataatccct tccagctaca gctccactca ggatgtaggc gtcttcaata
      481 ttggctgcga tgcaggacaa gatatcagct aaaagggttg tatcgtagtt cgtatagcgt
      541 ttgattagat gagacataaa acctccgggt tgcttattaa tattgctggc gaaatcagtc
      601 attgtgttag ctatgtaata ttaacagaag tgtaaagtgt ttttattccg gggtggtaat
      661 gttgcatata ttttcttagt gtgtgtcggg tgaatgaatt gcgttgcggt gaaaaaatcg
      721 ggcgcgtcag tgcggcctta ctgctgacgg cgttcccgga gttcggtcag tgctgcaagt
      781 aaattctccc accacgtttc ttccacgacc gggttttgtc gtgaaagcag ctgctgtgcc
      841 gactcaatca ttttcgtaag ctccctgtcg ctgatcctcc tgatgtcgtt catggtgtgc
      901 tccttcagtt tgctgtttct ggttatgttc atggtctttg ttacctgcgt gattgcctgt
      961 tggcggccga atgcacatag cgggcaattg ccgcttttcg gttaatcgat gccagtgttc
     1021 tggtgagttc tgcgttgtgt ttgtgtgcct gctcttcgtg gtactgccgc atgatttttc
     1081 cggcaggcgt gagcggctcc ggctccccgg gcggacggcg cggacgaaca gcgacaagcc
     1141 agctgcggtt agtgctgtcc ggtcgtccgt tgagtatgtc ggtgatgatg gccgtggtgg
     1201 tgcgatcggg actgacgacc gtgatttcca gctcatcgcc cggttgtggc cccgtggcaa
     1261 tgttgttcag ggtcagtaag ggcgtgcgct ggcgcatcat ttcgtcaaag cgctggcgct
     1321 gcatggcgca ataaaagcgg gtcattgtgg cgcctccgta ttcggtgcgg gggtgatatc
     1381 aatgcggcgg gtgctgtcat tccagtacgc ggtgcgtccg tcagccaggc gcacgcgggc
     1441 accgtaccgg acggaaacga cggcgctttc atgcagctgc acgcccagcg catcaagctc
     1501 gcgggtgatt tgtgcggtga tgtgtggctc agcggtcaga gcgacactca cgcgaacgcc
     1561 gccagcgggc gcactgatgc cgccgttttt cacggtttcc gtgtcaggtg gcggg
//
LOCUS       DAZGHF010000327         1615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGHF010000327 DAZGHF010000000
VERSION     DAZGHF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.330"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1515
                     /locus_tag="ACHK3A_005165"
     CDS             <1..1515
                     /locus_tag="ACHK3A_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8743092.1"
                     /translation="VRTTTSDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAE
                     DAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVE
                     SRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDHLTTVQTDTTRIQTV
                     YEPGSFTPLIRIETDNGEREKTQRRSLAEKLQQEGSEDGHGVVFPAELVRMLDRLEGE
                     IRADRVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLALISTEGA
                     TEWRGEYDEWGNLLNEENPHHLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGRYITQD
                     PIGLEGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSGDLISL
                     GGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSGVSNSV
                     GVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWVN"
BASE COUNT          410 a          368 c          497 g          340 t
ORIGIN      
        1 gtgcgcacga ccacatcaga cctggatatc cgcatcccgt atgccaccga cccggcggga
       61 aaccgcctgc cggacccgga gctgcacccg gacagtacac tcacagtgtg gccggataac
      121 cgcatcgcgg aggatgcgca ctatgtctac cgccacgatg aatacggcag gctgacggag
      181 aagacggacc gcatcccggc gggtgtgata cggacggacg acgagcggac ccaccactac
      241 cactacgaca gccagcaccg cctggtgttc cacacgcgga tacagcatgg cgagccactg
      301 gtcgagagcc gctacctcta cgacccgctg ggacggcgaa tggcgaaacg ggtatggcgg
      361 cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggaggt gacgtggtac
      421 ggctgggacg gcgaccacct gacgacggtg cagaccgaca ccacgcgtat ccagacggta
      481 tacgagccgg gaagcttcac accgctcatc cgcatcgaaa cagacaacgg cgagcgggag
      541 aaaacgcagc ggcggagcct ggcggagaag ctgcagcagg aaggaagcga ggacggccac
      601 ggtgtggtgt tcccggcaga acttgtgagg atgctggaca ggctggaggg ggaaatccgg
      661 gcagaccgcg tgagcagtga aagccgccag tggctggcac agtgcgggct gacggtggag
      721 cggctggcgg cacagataga gccggtttat ttacctgagc gaaaaattca tctttatcac
      781 tgtgaccacc gggggctgcc gctggcgctc atcagtaccg aaggagcaac ggaatggcgt
      841 ggggaatatg atgaatgggg caacctgctg aatgaggaga acccgcatca cctgcaccag
      901 ccataccgtc tgccggggca gcagcatgat gaggaatcag gactgtacta taaccggaac
      961 cggtactatg acccgctaca ggggaggtat attacacaag accccattgg gctggagggg
     1021 ggatggaacc tgtataatta cccactgaat ccgataataa ggatggatcc tttgggtttg
     1081 tataatttat atcaattatt atatgatgtt tggcatgatg attcatatgg aacatcatca
     1141 attgatatta ctggcagtgg agatctaata tcattaggtg gtcatgcagg acttggcgtt
     1201 gcgtttgcta aaaagaaagg tgaaatgtta tctgatattt gtatttatgc tacagcatgt
     1261 ggacatgcag gaattggtgg tgggataaat gcggctatca catattctga gaccaagtct
     1321 ttacctacat cgggagtcag taattcagta ggtgtaacgg ttggcggcgg agttgggggg
     1381 cattttgcgt atacttatgt agtggatgtt gataatccag aatcatcgac agaatctgtt
     1441 ggtatcggtg caggtgttga cgcttcagtt atgactctgg cttgtagaac gtggcaagaa
     1501 tgctgggtca attaacagga ggaaatatga aaaaatatat atttttagtt ttatttttat
     1561 tgtgtggttg ttctccagtg gacatagagc cagctagaca agaagttaat atctt
//
LOCUS       DAZGHF010000328         1611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGHF010000328 DAZGHF010000000
VERSION     DAZGHF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.331"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..340)
                     /locus_tag="ACHK3A_005166"
     CDS             complement(<1..340)
                     /locus_tag="ACHK3A_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO8743093.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNV"
     gene            656..>1611
                     /locus_tag="ACHK3A_005167"
     CDS             656..>1611
                     /locus_tag="ACHK3A_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8743094.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVL"
BASE COUNT          448 a          428 c          376 g          359 t
ORIGIN      
        1 gcacattacg cagacgaagg cgaccatccg gcgcatgaag catggccgtt ttcagttgct
       61 catgggggtc gttggagacg atgaggtcaa gccccctgac agccgtacgg gaaatgatgt
      121 tgtcaggatg actgaggtca gccgtgcgca tcagtaattc aaaaagaccg gccggcgctt
      181 catacgtcag cggaaagata ttgcttgccg tgggctgtgc atggtctccg tctatcagca
      241 gagtacggag gccggcatct gccagaaaac cggcaagatt agcagactgg gttgattttc
      301 cttcgccacc tttagtggat ataaccggta aaattttcat atagcacact ctttaaagta
      361 tgcaaccaat cacttacact acggaaacca gccagaatat tcacttcact acctgaatac
      421 tcgcaacacg gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccac
      481 tttcacttcc agattcatct cccgaactcc acgaaactca agtataatca catcgttaca
      541 agcaatctga acatcctgaa ctccaccgaa ctctactgaa ctctttctca atcaagcttt
      601 cattgggggc accggtgggg gctcattatg ttcagtcagt ttcaggagcc cccatatggc
      661 acttacagcc agacaggtcg aaacagccag acctaaagaa aaagactata aactctctga
      721 cgaacgtggt ttatatctgc tggtaaaaac cacgggagcc cgctactggc ggcttaaata
      781 ccggatagca ggaaaagaga aaaaactggc cctcggcgtc tatcccgacg tctcccttgc
      841 tgaggccaga atcaaacgtg acgatgcccg aaaaatcatc tccgaaggtg gtgacccggg
      901 cgaaaagaag cgaaaggaaa aactcactca gaaaatctct gccaccaata cgttccatgc
      961 cctcgctacg gaatggcacc agcataaatc tttgtcatgg tctgaaagtt acgccagaag
     1021 cgtactggaa gcgctggata aagatatttt cccgtatctg ggcaaacgaa gcgttacgga
     1081 tatcctcccg ctggaaatgc tggaaattct gcgccgcata gaaaaacgtg gctcgctgga
     1141 aaaactgcgt aaggtacgtc aatactgtaa tcagattttt cgttatgcca tcgccaccgg
     1201 acgagccact gtcaatccgg catctgaact gaccagtacg ctggcggcgc caaaagctgc
     1261 acacttcccc cacctgagag cagatgagct ccctgttttt ctcaggaagc tcgctgaata
     1321 tcatggcagt cctgttaccc gcatggcgac aaatctgctg cttctgacag gcctcagaac
     1381 gattgagcta cggtccgctg aatggtcaga aattgatttt gataatgccc tgtggacaat
     1441 ccctgaaagc cgcatgaaaa tgcgacgtaa acatgtcgta ccactgtcac gacaggtcac
     1501 tgacattctg caacaactca aaactttctc cggacaatac cggctggttt tcccgggacg
     1561 ttgtgatatc aacaagccaa tgagcgaagc cagcatcaat atggtgctca a
//
LOCUS       DAZGHF010000329         1603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGHF010000329 DAZGHF010000000
VERSION     DAZGHF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.332"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(6..>1603)
                     /locus_tag="ACHK3A_005168"
     CDS             complement(6..>1603)
                     /locus_tag="ACHK3A_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO8743095.1"
                     /translation="MDERQRRENVDIYGKATFFDDGWLVKKGETWTVNKGVGYVEGLR
                     AELTEDTPMVTPTSPSRIWLNVWWTGTLTSEWTVEHEIIPADTDVTEERLRKTYPRDG
                     IQQYFYAIAEIDEDGNVTDLRPRGTPEERRSLDALKKHEKSRNHPDATLKEKGFTQLS
                     SATTSTSETQAATPKAIKIAMDNADGRLAKNRNGEDIPNKELFLINVGAPRVYAKDGY
                     VGEAGQRWTTEQFVEWLNERGAFRPGLWIFKANISLSRHSVLTDTGYGDICLGGCLIE
                     VSGDINGGIIRITTGLESPNPADMYLRTQFTGVVLNKGKSTIWYRTYTDKNKPTPQDI
                     GALPASELLPAGVPLPWPSDTPPAGYAIMVGQTFDKARYPLLAKAYPDGRIPDMRGWT
                     IKGKPASGRAVLSQEQDGIKSHTHGGSVSNTDLGTKTTSSFDYGTKTTSTNGQHEHSV
                     PLRRQDGGGINFDWLDGSSSGSHIGNGYVPAAGDHQHTVPIGAHTHTLALGSHGHGLT
                     INPTGNAENTVKNIAFNYIVRLA"
BASE COUNT          286 a          418 c          448 g          451 t
ORIGIN      
        1 ggtcattatg caagcctcac gatgtagtta aatgcaatat ttttaacggt gttttcggca
       61 ttaccggtgg ggttaatggt caggccgtga ccatgtgaac ccagtgcgag cgtgtgggtg
      121 tgtgcgccaa tgggtacggt atgttggtgg tcacctgcgg caggaacgta tccattacca
      181 atatgagaac cggacgatga accatccagc cagtcaaagt ttatgcctcc gccatcttgt
      241 cttctcaggg ggacgctgtg ttcatgttgt ccgtttgtag aggttgtttt ggttccgtag
      301 tcaaatgatg aggtcgtttt cgtgccaagg tcagtatttg agacactccc cccgtgggtg
      361 tgggatttga tgccgtcctg ttcctgtgac aataccgcac gaccgctggc aggttttccc
      421 ttgattgtcc agccacgcat atccgggata cgaccatccg gataggcttt tgccagcagg
      481 ggatatcgtg ctttatcgaa tgtctgcccc accatgatgg cgtaaccggc aggcggggtg
      541 tctgacggcc acggcagggg aacgcctgcg ggtaacaact cacttgcagg cagtgcgccg
      601 atgtcctgtg gtgtgggttt gtttttgtcc gtgtaagtgc ggtaccagat ggtgcttttc
      661 cctttattta aaacaacccc ggtgaactgt gtgcggaggt acatgtcggc agggtttggg
      721 ctttcgagac ctgttgtgat cctgatgatc cccccgttga tgtcgcctga tacttcgata
      781 aggcagcctc caaggcagat atcaccatag cccgtatcag tgagcacgct gtgccgggaa
      841 agtgaaatat tggctttgaa tatccagagt cctggtctga aggcgccacg ctcattcagc
      901 cattccacga attgttctgt tgtccagcgt tgccctgctt cacccacgta cccgtctttt
      961 gcataaacgc gtggtgcgcc cacgttgatc aggaacagct ctttgttggg gatgtcctca
     1021 ccattgcggt ttttcgcaag ccgaccatcg gcgttgtcca tggcgatctt tatagccttt
     1081 ggcgtggctg cctgtgtctc tgatgtactg gtggtggcgc tgcttaactg tgtgaagccc
     1141 ttttctttca gcgttgcgtc cgggtggttg cgtgatttct cgtgtttttt cagggcgtcc
     1201 agactgcggc gctcttccgg ggttcctctg gggcgcaggt cagtcacgtt gccgtcttca
     1261 tcaatttcag cgatggcata aaaatattgc tgaatgccgt ccctgggata tgttttccgt
     1321 aaacgctcct ccgtaacgtc cgtgtcggct ggtatgattt cgtgctccac cgtccattca
     1381 ctggtgagcg taccggtcca ccacacattc agccagatac gggacgggga ggtgggcgtg
     1441 accattggtg tgtcttcggt cagctctgcg cgtagcccct ccacgtaacc cacccctttg
     1501 ttgactgtcc aggtttcgcc tttcttaacc agccacccgt catcaaaaaa agttgccttc
     1561 ccgtagatat cgacgttttc ccggcgctgg cgttcgtcca tgg
//
LOCUS       DAZGHF010000330         1587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGHF010000330 DAZGHF010000000
VERSION     DAZGHF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.333"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..183
                     /locus_tag="ACHK3A_005169"
     CDS             <1..183
                     /locus_tag="ACHK3A_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO8743096.1"
                     /translation="DGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVK
                     TKVKGTVVMFSASGKK"
     gene            complement(265..607)
                     /locus_tag="ACHK3A_005170"
                     /pseudo
     CDS             complement(265..607)
                     /locus_tag="ACHK3A_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            620..1215
                     /locus_tag="ACHK3A_005171"
                     /pseudo
     CDS             620..1215
                     /locus_tag="ACHK3A_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          486 a          376 c          347 g          378 t
ORIGIN      
        1 gacggcgttg tgcacaatag cttcggtcaa aaactgatgc gcatttacaa tcagaaaggc
       61 atcttttcaa acacaaaaga ttccgaagaa ggcttaacac atatactctc agagcatttc
      121 gagaatgtta aaaccaaggt taaaggtact gtagtaatgt tttccgcttc agggaaaaaa
      181 tagcatccag ccgcagcacg ttcttgcgca cgacgttctg cggcataatc ccaatgatta
      241 ctctctgaca gggttcacag gccactaaat atcaggggca gttgatgtat caactcggtt
      301 cagcaacacc cgatacatct tccaggcttc cagcaacgag gtttcttcct ccgtatcctt
      361 tatccatgat atgccgttcc acttctgaaa ctcccctgcc ggggatacct ggtgacattt
      421 tccggtaacg gaccgagttc agaaataaat aatgcgtccc ctgatgccac gtcataaacc
      481 gttttacccc gatggtcttc aacgagatgc cacgatgcct catcactgtt gaaaacaacc
      541 acgaagccag ccggaatatc tgggcaatat cagtactgtt tgcaggcaaa cctgtatgag
      601 gtggaatggc acaagagact tacaaaagaa tcaggccaaa cgcctgattg atgagagccc
      661 cgaaccactg aaatctgtcg tcaaatttgc gctggtaact gttctgagaa agtcgaacat
      721 cataaatctg gagtggcaac agattgacat gcagcgacaa gttgccaggg tgaatccaga
      781 agatagcaaa tcaaactgcg ccattggtgt ggcgctgaac gataccgcct ctaaagtgtt
      841 gcgtgatcaa acaggcaagc atcacaaatg tgggtgtaca taccagggcg gctaagcgag
      901 cagatggaac atcaatgcct gcgctcagga agatgcgcat tgacagcaag acagcatggc
      961 tatcagcttg tcgtcgtgca ggaattgaaa atttccgttt ccatgacctc tggcatacct
     1021 gggcaagctg gttgattccg tcaggcgtcc cattatcagt gcttcaggaa atgggcggat
     1081 gggagtccag agaaatggtt cgtagatatg ctcaccttgc gcctaatcat ttgactgagc
     1141 acgcgaggaa aatagacgac atttttggtg ataatgtccc actctggaat tatagaagaa
     1201 ataaagaagg cgtaactgat tgaattttaa tggcgcgccc tgcaggattc gaacctgcgg
     1261 cccacgactt agaagttcct agaacgacat tttaagtcaa caacttaccg cgccatctct
     1321 gcgctcacac gtcccactac ctcaaaacat gtaaagcctt gcaagccatt gcgaggcctt
     1381 atgtgtctca gttttgtccc tcttttttgt actaaaaaac atagtaattg aggataaacc
     1441 tcatgctatt ttcgcttata tgcctctaaa ggcatggcac ttaaatagat aaaagcacca
     1501 caaaagcata aaaaaaccac acagtaaaac cgcaatatga aacaataaca gataattaaa
     1561 ccaaaaacag atagcgcatt gtgataa
//
LOCUS       DAZGHF010000331         1579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGHF010000331 DAZGHF010000000
VERSION     DAZGHF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.334"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..831
                     /locus_tag="ACHK3A_005172"
     CDS             <1..831
                     /locus_tag="ACHK3A_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO8743097.1"
                     /translation="IPEGTIVQTARINGVIYSMTVTEETLISSGKSGASVPVEATTEG
                     SGHNLAPGYFRILPVAIPGIASVVNREDWLTVPGADEESDDELRDRIRNQFNLVSSYH
                     TDAVYRGLIASVAGLSIDRIYFQHDAPRGPGTANAYLLLDSGEASAPFIEAVNRYINE
                     EGHHGHGDDLQCFAMPETRHRLDVTVYIRSSTTLPPDGAEGLKQDISDLIRCAFRENA
                     RFNVKKVWPYSRFSFSTLGKEIHQTFPVVASVQFSLEDIISDLAVPRLESLTVEVVIE
                     "
     gene            824..1372
                     /locus_tag="ACHK3A_005173"
     CDS             824..1372
                     /locus_tag="ACHK3A_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743098.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAAQAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTSLYRRRIAHAFVNAQDAGEVAGFIAIFE
                     RLGIGHVELQERKPGVDWDVILVQVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     NPFIIHYGAYEGEYICWTARAE"
     gene            1395..>1579
                     /locus_tag="ACHK3A_005174"
     CDS             1395..>1579
                     /locus_tag="ACHK3A_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743099.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFIFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVG"
BASE COUNT          358 a          456 c          450 g          315 t
ORIGIN      
        1 atccccgaag gcaccatcgt ccagaccgcc agaattaacg gcgtgatata cagcatgacc
       61 gtcacggaag aaacgcttat cagcagcggt aaaagcggcg catccgttcc cgtggaagcc
      121 accaccgaag gcagcgggca caacctcgcg ccggggtact tccgtatcct tcccgttgcc
      181 attcccggca ttgcatccgt ggtgaaccgg gaagactggc tgaccgtacc gggtgccgat
      241 gaggaaagcg acgacgaact gcgcgacaga atacgcaacc agtttaacct cgtgagcagt
      301 taccacacgg acgccgtcta ccggggactt atcgccagcg tggcgggcct gagcatcgac
      361 cggatctact ttcagcacga cgccccgaga ggaccgggaa cggccaacgc ctacctgtta
      421 ctggacagcg gcgaggcctc tgcccctttc attgaagcag tcaaccgcta catcaacgaa
      481 gaggggcacc acggacacgg ggatgatttg cagtgtttcg ccatgccgga gacgcgtcac
      541 cgtctggacg tcaccgtata catcagaagc tcaaccaccc tgccaccgga cggcgcggaa
      601 gggctgaaac aggatatcag cgacctgata cgctgcgctt ttcgggagaa cgcccgcttt
      661 aatgtgaaaa aagtctggcc ctattcgcgc ttttcgtttt ccacgctggg caaggaaatt
      721 catcagacct tcccggtggt ggcttcggtt cagttttcac tggaagacat catcagcgat
      781 ctggccgtgc cacggctcga atccctgaca gtggaggtgg tcattgaatg actttttgca
      841 gcgacttcgc tcgctgaaac tgccctcctg gatggacagg ggggaacccg acaaactgct
      901 gcgggccgcg caggcgttct ggtcccgcgt atgggaatgg atcacctggc ccgtgcgcca
      961 gtttgacccc atgacatgtg cagagcccgt acttgattta ctggcatggg agcgggacat
     1021 cacccgtttt aaaggtgaac ccacatcact gtaccgccgc cgtatcgccc acgcctttgt
     1081 caatgcacag gacgccggag aggtggcagg atttattgcc atttttgaac ggctgggcat
     1141 tggacatgtg gagttgcagg agcgcaagcc cggcgtggac tgggatgtga tcctggtgca
     1201 ggtgacagac agccagatag ccgacaacag cgatcttctt ctggagatca tccgcaaata
     1261 tggcaggaca tgccgccgtt accgttttga ggtggaaacc cttaacccct tcatcatcca
     1321 ttatggcgca tacgaagggg agtatatctg ctggacggcc agggcagagt gaatgactga
     1381 aggagtatga ctttatggcg aaatctgtaa ttaccagcgc ctttatccgg ctcaaggcac
     1441 agcaggcggc ggaaggcacc cccgttgtgc tggatgagtt tatttttgca aacgtcccgg
     1501 acctcaaccc ggaaacaccg gtatcccccg acgaaactct gccacctgaa gatcagattg
     1561 ttcaccgcca ggccgttgg
//
LOCUS       DAZGHF010000332         1579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGHF010000332 DAZGHF010000000
VERSION     DAZGHF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.335"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..831
                     /locus_tag="ACHK3A_005175"
     CDS             <1..831
                     /locus_tag="ACHK3A_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO8743100.1"
                     /translation="IPEGTIVQTARINGVIYSMAVTEETLISSGKSGASVPVEATTEG
                     SGHNLAPGYFRILPVAIPGIASVVNREDWLTVPGADEESDDELRDRIRNQFNLVSSYH
                     TDAVYRGLIASVAGLSIDRIYFQHDAPRGPGTANAYLLLDSGEASAPFIEAVNRYINE
                     EGHHGHGDDLQCFAMPETRHRLDVTAYIRSSTTLPPDGAEGLKQDIGDLIRCAFRENA
                     RFNVKKVWPYSRFSFSTLGKEIHQTFPVVASVQFSLEDIISDLAVPRLESLTVEVVIE
                     "
     gene            824..1372
                     /locus_tag="ACHK3A_005176"
     CDS             824..1372
                     /locus_tag="ACHK3A_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743101.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAARAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTEIYRRRISYAFINAQEAGEVAGFIAIFE
                     RLGIGYVELLERQPGVDWDVILVRVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     TPFIIHYGAYEGEYICWTARAE"
     gene            1395..>1579
                     /locus_tag="ACHK3A_005177"
     CDS             1395..>1579
                     /locus_tag="ACHK3A_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743102.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFIFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVG"
BASE COUNT          347 a          460 c          458 g          314 t
ORIGIN      
        1 atccccgaag gcaccatcgt ccagaccgcc agaattaacg gcgtgatata cagcatggcc
       61 gtcacggaag aaacgcttat cagcagcggt aaaagcggcg catccgttcc cgtggaagcc
      121 accaccgaag gcagcgggca caatctcgcg ccggggtatt ttcgtatcct tcccgttgcc
      181 attcccggca ttgcatccgt ggtgaaccgg gaggactggc tgaccgtacc gggtgccgat
      241 gaggaaagcg acgacgaact gcgcgacaga atacgcaacc agtttaacct cgtgagcagt
      301 taccacacgg acgccgtcta ccggggactt atcgccagcg tggcgggcct gagcatcgac
      361 cggatctact ttcagcatga cgccccgaga ggaccgggaa cggccaacgc ctacctgtta
      421 ctggacagcg gcgaggcctc tgcccctttc attgaagcag tcaaccgcta catcaacgaa
      481 gaggggcacc acggacacgg ggatgatttg cagtgtttcg ccatgccgga gacgcgtcac
      541 cgtctggacg tcaccgcata catcagaagt tccaccaccc tgccaccgga cggcgcggaa
      601 gggctgaaac aggatatcgg cgacctgata cgctgcgctt tccgggagaa cgcccgcttt
      661 aatgtgaaaa aagtctggcc ctattcgcgc ttttcgtttt ccacgctggg caaggaaatt
      721 catcagacct tcccggtggt ggcttcggtt cagttttcac tggaagacat catcagcgat
      781 ctggccgtgc cacggctcga atccctgaca gtggaggtgg tcattgaatg actttttgca
      841 gcggcttcgc tcgctgaaac tgccctcatg gatggacagg ggagaacccg acaaactgct
      901 gcgggccgcc cgggcgttct ggtcccgcgt atgggaatgg atcacctggc ccgtgcgcca
      961 gtttgacccc atgacatgtg cagagcctgt acttgatttg ctggcatggg agcgggacat
     1021 cacccgcttt aaaggggaac ccacggagat ctaccgccgc cgcatcagtt acgccttcat
     1081 caatgcacag gaggctggcg aggtggcggg ctttatcgcc atttttgaac ggctgggcat
     1141 cggctacgtg gagttactcg agcgccagcc cggcgtggac tgggatgtga tcctggtgcg
     1201 ggtgacagac agccagatag ccgacaacag cgatcttctt ctggagatca tccgcaaata
     1261 tggcaggaca tgccgccgtt accgttttga agtggaaacc ctcaccccct tcatcatcca
     1321 ttatggcgca tacgaagggg agtatatctg ctggacggcc agggcagagt gaatgactga
     1381 aggagtatga ctttatggcg aaatctgtaa ttaccagcgc ctttatccgg ctcaaggcac
     1441 agcaggcggc ggaaggcacc cccgttgtgc tggatgagtt tatttttgca aacgtcccgg
     1501 acctcaaccc ggaaacgccg gtatcccccg acgaaaccct gccgcctgaa gatcagattg
     1561 ttcaccgcca ggccgttgg
//
LOCUS       DAZGHF010000333         1565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGHF010000333 DAZGHF010000000
VERSION     DAZGHF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.336"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            330..464
                     /locus_tag="ACHK3A_005178"
     CDS             330..464
                     /locus_tag="ACHK3A_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743103.1"
                     /translation="MSLRQIVTLADAVVKGQPTRIPPMKGDEWREFCWWLDHLRGYAM
                     "
     gene            525..1007
                     /locus_tag="ACHK3A_005179"
     CDS             525..1007
                     /locus_tag="ACHK3A_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743104.1"
                     /translation="MTVVQNAVLRKTQLVFGAVAGIQELKSKRLQKKCRALAEREKPF
                     RIMEFTNFLNCGQHRLFVLRNVEGEIPHTGILPDVKAFSGGVITGKRFIGLIVVDRVE
                     EKILVYQYARSNWTDRKFEENIRISYDSPVFALKHFTKVNIWFWQRELHILLNKTRCR
                     "
     gene            1085..1486
                     /locus_tag="ACHK3A_005180"
     CDS             1085..1486
                     /locus_tag="ACHK3A_005180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743105.1"
                     /translation="MRKNEDNILFVPAGMTREDMVSEIALLRSSRDLTTDIYFEYVDA
                     GFPETTELSAADTGRLKAGLPAHHFHWLGADGAPLTVRRISDFADVADDFSCLAAAID
                     PELAEPPEEGIPVEGFNGGIFCVIREEDTDK"
BASE COUNT          429 a          353 c          442 g          341 t
ORIGIN      
        1 gtggcagtga tgccccatgc ggcgaaggtt ctcaaacggg tgatggtgaa taccggaggg
       61 aacaaaaggc aggtccccgc aaaacgtcaa aaccgcggct cccgaaactc aacgggtgat
      121 ggttttaaaa agacaaaacc cggttcttta acaatctgac aatgcaatga aaatcactgg
      181 attatgcagc aattaaggag gtgacacgat gtaggcgcaa tacagcgcaa gggaaagcac
      241 ggggaggaaa aggacgttca cgggtatgca agggggtgta ggagcacccc tttgcacgaa
      301 tatcaacaac ctttgtaagg atattgatta tgtcactcag acaaatcgta acccttgctg
      361 atgcggttgt caaagggcaa cccactcgca tcccgccaat gaaaggcgac gaatggcgcg
      421 agttctgttg gtggctggac cacctcagag gctatgccat gtgactcccg gggcggtaac
      481 gccccctttt aaacggtttc atttagcccc tgacggaggc gattatgaca gtagtgcaga
      541 atgcagtgtt gcggaagaca cagcttgttt ttggtgctgt ggccggaata caggaattaa
      601 aaagtaaacg gcttcagaaa aagtgccggg cgctggcgga gcgtgaaaaa ccgtttcgca
      661 tcatggagtt cacgaatttt ttaaactgtg gtcagcatcg tttatttgtg ttgcggaacg
      721 tggaaggtga aataccgcac acagggatac tgccggatgt gaaagcattt tcaggcggag
      781 taataaccgg aaagcggttt attggtctga tagtcgttga ccgcgtggag gaaaaaatcc
      841 tggtttatca gtatgcacgc agtaactgga cagaccggaa atttgaagaa aatatccgaa
      901 tcagctatga cagcccggtt tttgctctga agcatttcac gaaggtgaac atctggttct
      961 ggcaaaggga attacatatt ctgttgaaca aaaccaggtg cagataaatc cgggcggggc
     1021 ctgacggtcc cgcccttctg acgcagagcg ggcgacaccg tgatactgaa tgagagacga
     1081 ggcaatgaga aaaaacgaag ataatatcct gtttgtgccc gcaggcatga cgcgtgaaga
     1141 tatggtcagc gagattgctc tgctacgttc ttcacgcgac ctgacgacgg atatttattt
     1201 tgaatacgtg gatgcgggat ttcccgaaac aaccgaactc tctgccgcag acaccggacg
     1261 cctgaaagcc gggttaccag ctcatcattt tcactggtta ggcgcggatg gtgctcccct
     1321 gacggtaagg cgaataagtg actttgccga tgttgctgat gatttcagct gtctggcagc
     1381 cgccatcgac ccggaactgg ctgagccacc ggaggagggt ataccagtgg aaggatttaa
     1441 cggggggatt ttctgcgtaa ttcgggaaga ggatacagat aaataaccct caatggcaac
     1501 ctcaggcatc cgccagcaaa aatatgaaag gggccgcgct cacgcgccgc ccctttcatt
     1561 acgcg
//
LOCUS       DAZGHF010000334         1554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGHF010000334 DAZGHF010000000
VERSION     DAZGHF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.337"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(83..1051)
                     /locus_tag="ACHK3A_005181"
     CDS             complement(83..1051)
                     /locus_tag="ACHK3A_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO8743106.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSALTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            complement(1053..>1554)
                     /locus_tag="ACHK3A_005182"
     CDS             complement(1053..>1554)
                     /locus_tag="ACHK3A_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO8743107.1"
                     /translation="CHFMKPAGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYT
                     KSDKQAWWSQIKFYQRQRASLGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSN
                     YIRHKFIRFARGRSRAEKMTEKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKN
                     RNSLGQ"
BASE COUNT          349 a          423 c          359 g          423 t
ORIGIN      
        1 ggtttgatat ttgccggaac ggcgctgtac gctgccgtcg ggatagtgaa taaagcttta
       61 tgtacctgat tgtccgtacg catcacgcaa ccgccttttt tctgtaaaaa accagttccc
      121 gaacctgatc gccgttcatg agcatattgt tgaaatcatc atgatcaggc caccggacac
      181 ttacccgctg aatgtcattt cgggcctgga gatttgcatg tgcgcattca caggccgcag
      241 ccattccggt agcactgtat tcatcgcgat cggcaaaaat aatcagatgg gtgactcccg
      301 ccggtacgcg gaactttttc ataaaaccac tgttaatggt tgcccaggta tttactttgt
      361 aaatctgatg tgcggacaac gcggtttcga taccttcagc tatacccagt gtactggcta
      421 ccggaaacat cctgatagca actgaccggg catggtccag atagttatcg tcctgtaaag
      481 atttgagcct tctggcgctg ataccaatgt ctgctttttt agctccatca agcaatgtct
      541 gatgcaggta acacagttct cccttgtcat cggtcgccag ggcataaaga gactggaaca
      601 tgcgcccgtt ataattttgc tggctgttga agcgaactcc ctcaaccgga agactgaata
      661 tgccacggga acgaagatat tcagcaccag atgttccctg tagcgccgtg agcgctgaaa
      721 atcgacgctg aacgcgcgat ctcaggcttt ccgacgtaca gatcacaggc ggtttatctc
      781 tccggtattc attccccgtg atgcggtcta tttcagcgca aatttcatga aacgctcttt
      841 tttgtgtcag agcgatcagt ttcatgccgt caccgctacc acaaacacag atccacgttc
      901 cggtgccatc acggtcatca atacggaact tacctctggc accacaaacc ggacattccc
      961 ctttgaagtg ctttttaccc gtgactggcg gcagtccgta atgttttaaa atttcaggcc
     1021 agtggccttt cgctgcttct gctgttttca tcttactgac ccagactgtt tctgtttttc
     1081 tgaagttgtt gctttgcctg catgatctgc cacgactcac tgccttccgg caccccagac
     1141 cggacaccat gaagcaggga cagagaaagc gcttcggggg atttttccgt catcttctct
     1201 gcccggctac gccctctggc aaaccggatg aatttgtgtc tgatgtaatt actgacctcc
     1261 ggggtgattt ccatcggaaa actgctcagc ccgtcaggcc attcgccgaa cttctcccgg
     1321 aaagtgtgag cgcaccatcc atcgctgacc ggtcgcccca gagaggcacg ctgacgctga
     1381 tagaatttga tctgactcca ccaggcctgt ttgtcgcttt tggtgtaaat tttttcgtcc
     1441 ttgctgagtt tcctgatatt gcggcgggta tctgtgttca catcctcacc agccagcggt
     1501 ttaaatccgc atttcggaca gacataaaca cctgccggtt tcatgaaatg acac
//
LOCUS       DAZGHF010000335         1546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGHF010000335 DAZGHF010000000
VERSION     DAZGHF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.338"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1352
                     /locus_tag="ACHK3A_005183"
     CDS             <1..1352
                     /locus_tag="ACHK3A_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO8743108.1"
                     /translation="FRINAPAAPASIELTPGYYQITAVPHLTVYDPTVQFEFWFSEAK
                     IADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASND
                     AEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTLED
                     QSATIQQIQRVQADTNNDLAALYMLKVQKTKNGIPYVAGIGAGIEDADGQPLSNILLQ
                     ADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLT
                     ARNADISGHISANSGTLNNVVIAENCTINGTLKAENIIGDLVKCAGVAFPVDGSYLAN
                     GTRTLTVYDDHSFDRQIIIPPIIYVGSKQESRTSNDIWTECFLHVDQNGRRIYSGRSV
                     TEPGIFSGIIDMPAGGGHITLSFTVSSRRQNGSFGSSRISNLQAIVVKKNSAGISIR"
BASE COUNT          396 a          372 c          461 g          317 t
ORIGIN      
        1 cgttccggat taacgcaccg gcagcaccgg ccagcattga actgacgccg gggtattatc
       61 agataacggc ggtcccgcat cttacggtgt atgacccgac ggtacagttt gagttctggt
      121 tttcggaggc aaaaatcgca gacacatctc aggtggaaac ctctgcccgt tatctgggga
      181 caggcagcca gtggagtgtc tccggcccgc acattaagcc ggggaaggat ttctggtttt
      241 atgtgcgcag cgtcaatctg gtggggaaat ctgcgtttgt ggaagccagt ggccgggcca
      301 gcaatgatgc agaagggtat ctggactttt tcagaggaga aatcgggaag acacatctgg
      361 cacaggggat gtgggagctg attgataaca gccagcttga tgatgagatg gcggagatga
      421 agaccaccat caccgaaacc cgcaatgaaa tcacgcagac ggtcagtaaa accctggaag
      481 accagagtgc caccatacag cagatacagc gggtgcaggc agacacaaat aacgacctgg
      541 ctgcgctgta catgctgaag gtgcagaaaa caaaaaacgg cattccgtat gttgccggta
      601 ttggtgcggg gattgaggat gctgatggcc agcccttgag caatatactg ctgcaggcgg
      661 accgtatcgc gatgattaac ccggagaacg gcaacaccac gccgctgttt gtggcgcagg
      721 ggaatcagct gttcatgaac gacgtgttcc tgaagcgact gtttgcagtg agcatcacct
      781 cgtccggcaa tcccccgacg ttctccctga cgccggaggg caggctgacg gcccgcaatg
      841 ctgacatcag cggacatatc agtgcgaact cgggcacgct gaataatgtc gtgatagcgg
      901 agaactgtac gataaatggc acgctgaaag cggagaacat tattggtgat cttgtgaaat
      961 gtgcaggggt ggcttttccg gtggatggta gttaccttgc gaacggtaca cgaacgctga
     1021 cggtgtatga cgatcacagc tttgaccggc agattataat cccgccgata atctatgtcg
     1081 ggtcaaaaca ggaatcccgc accagtaatg acatctggac agagtgcttc ctgcatgttg
     1141 atcagaacgg acgccggatt tattccggca ggtcagtgac agagccggga attttcagcg
     1201 ggatcatcga tatgccagcc ggaggtggtc atatcaccct gagttttacc gtaagttcac
     1261 ggcgtcagaa cgggagtttt ggcagttcac gtatcagtaa ccttcaggcg atagtggtga
     1321 agaaaaacag cgcggggatc agtatccgct gaacatcgcg ccccgggatt gccggaagga
     1381 tacaaaaacc gtacattatg tgcgggtgcc tttggctgat ggccggaggg aacacctgaa
     1441 ggccggaaag acgaaggccc cggacaaaca ttcatgttta acccgaggcc tgacgttctt
     1501 ccttcagcaa gaagaaggtt agcgcctctc cgtaaaagga gcaagc
//
LOCUS       DAZGHF010000336         1531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGHF010000336 DAZGHF010000000
VERSION     DAZGHF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.339"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..227)
                     /locus_tag="ACHK3A_005184"
     CDS             complement(<1..227)
                     /locus_tag="ACHK3A_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO8743109.1"
                     /translation="MQEVTQNAPESPAETQCDQLTDAPETAHQKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGK"
     gene            335..634
                     /locus_tag="ACHK3A_005185"
     CDS             335..634
                     /locus_tag="ACHK3A_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8743110.1"
                     /translation="MSTNVGEKIRIMRKSEMLTQEKMAEITGLTVAALRQYEQGRNEP
                     NLESTKKLLKSPVFRKYRDWFLFDEVEPKAGQVVPALAHIGRSESESPHSGKKTG"
     gene            701..>1531
                     /locus_tag="ACHK3A_005186"
     CDS             701..>1531
                     /locus_tag="ACHK3A_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO8743111.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRLELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKDLESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     F"
BASE COUNT          404 a          305 c          396 g          426 t
ORIGIN      
        1 aatttccctg cttttgccat gctcacgatg gcgtcaacgg tcttgccgat atacatcgca
       61 aacccttctt ttgacaggag atgagaaggg ttttgtgaaa gcctgatttc ctggcgttca
      121 ggggctttgc gcccggtctt ttggtgtgcg gtttctggcg catctgtcaa ctgatcgcac
      181 tgtgtttcgg caggcgattc aggcgcgttt tgagtgactt cttgcatctg gtatcctccg
      241 ttattggtgc tatttctatt tagcaccagt tagagccgcc tagattgtct ctaggtgtgt
      301 ttgttgatgt ataaactaag agagttaact cactatgtca actaacgtag gtgaaaaaat
      361 tagaatcatg cgtaagtctg aaatgcttac acaagaaaaa atggctgaaa ttacagggct
      421 cacggtagcg gccctgcgtc aatatgaaca ggggagaaat gagccaaatc ttgaatccac
      481 aaaaaagctg ctcaagtctc ccgtcttccg taaataccgt gactggtttt tgttcgatga
      541 ggtcgaacca aaggccgggc aggtggtacc ggctctcgca cacattgggc gaagcgaatc
      601 ggaatcaccc cactccggca agaagactgg ttaacggtcc atctggcctt tatttctcaa
      661 aattgtgatc aagatgatgt tttcattggg gggcttcctt atgtcgatta agaagctcgc
      721 tgatggcaaa tactgtgtgg acgtgcggcc tgctggatct gaaggaagaa gatttcggcg
      781 gcgatttcct acccgtggtg aagcggcgat gtatgaacgc catgttctga cgcattatca
      841 cgataaggac tgggtcgaaa aacctattga acgtaaagcg ttaagcgatc ttctggaact
      901 gtggtgggtg taccacggta agaatcatcg ttatggcgcg atgaatcggg tcagaattga
      961 agcggttctt cgtgatatgc aagagcttgg tattaacaga agtgaccact taacccgaaa
     1021 aaatattatc agatatcgcc ttgaattaat gaatcgcgga attaagcagt caacggtaaa
     1081 tcgttattgt gcaatgatga gcggtttctt tgaaaagctg attgatgttg aagagtttgt
     1141 gggtgataac ccgtttcacg agatacgcaa gctaacaatt aatcagccgg aaatggctta
     1201 tcttgcacat gatgatattc ccaggctaac cgcactttta tctggcgata atcttaaggc
     1261 tgtattgtta agcctggcta caggcgggcg ctggggtgaa gttgccaata ttaaaggtga
     1321 acacataatt ggtgggaagg ttattttcat ggagacaaag aatggatcgc ggcgggttat
     1381 tcctatagct aaagatcttg aatctctgat taaagtcaaa gcgactggaa gattaatgaa
     1441 ccccagttat gcgatagtca gaagtgcaat aaggactgtg cggcctgatt taccagtcgg
     1501 tcagtctgtg catgttttac ggcacacatt c
//
LOCUS       DAZGHF010000337         1518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGHF010000337 DAZGHF010000000
VERSION     DAZGHF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.340"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1358
                     /locus_tag="ACHK3A_005187"
     CDS             <1..1358
                     /locus_tag="ACHK3A_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO8743112.1"
                     /translation="FRINAPAAPASIELTPGYYQITAVPVLAVYDPTVQFEFWFSEAK
                     IADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASND
                     AEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTLED
                     QSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIGAGIEDADGQPLSNILLQ
                     ADRIAMINPENGNTTPMFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLT
                     ARNADISGHISANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVGKAFPRDSRAPER
                     WPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHNDIYSSCRLMVKCNGRVLFERT
                     ALAESQVYSGVIDMPAGRGHMTLEFSVSAWLVNGWYPTASISDLLVVVMKKSTAGISI
                     S"
BASE COUNT          374 a          361 c          466 g          317 t
ORIGIN      
        1 cgttccggat taatgcgcct gcagcaccgg ccagcattga gctgacgccg gggtattatc
       61 agataacagc agtaccggtg ctggcggtgt atgacccgac ggtacagttt gagttctggt
      121 tctcggaggc aaaaatcgca gacacatctc aggtggaaac ctctgcccgt tatctgggga
      181 caggcagcca gtggagtgtc tccggcccgc acattaagcc ggggaaggat ttctggtttt
      241 atgtgcgcag cgtcaacctg gtggggaaat ctgcgtttgt ggaagccagt ggccgggcca
      301 gcaatgatgc agaagggtat ctggactttt tcagaggaga aatcgggaag acacatctgg
      361 cacaggggat gtgggagctg attgataaca gccagcttga tgatgagatg gcggagatga
      421 agaccaccat caccgaaacc cgcaatgaaa tcacacagac ggtcagtaaa accctggaag
      481 accagagcgc caccatacag cagatacagc gggtgcagac agacacaaat aacgacctgg
      541 ctgcgctgta catgctgaag gtgcagaaaa caaaaaacgg cattccgtat gttgccggta
      601 taggtgcggg gattgaggat gctgatggcc agcccctgag caatattctg ctgcaggcgg
      661 accgtatcgc gatgattaac ccggagaacg gcaacaccac gccgatgttt gtggcgcagg
      721 ggaatcagct gttcatgaac gatgtgttcc tgaagcggtt gtttgcagtg agcatcacct
      781 cgtccggcaa tcccccgacg ttctccctga cgccggaggg caggctgacg gcccgcaatg
      841 cggacatcag cggacatatc agtgcgaact cgggcacgct caataatgtg accattaacc
      901 agaactgccg gatacttgga aaactgtctg ccaaccagat tgaaggcgat attgttaaaa
      961 ccgtggggaa ggctttcccg cgggactccc gcgcaccgga gcggtggcca tcggggacca
     1021 ttaccgtcag gatttatgac gatcagcctt ttgaccggca aattgtgatc ccggcagtgg
     1081 ctttcagcgg cgctaaacat gagagagagc ataacgatat ttattcatca tgccggctga
     1141 tggtgaaatg taacggcagg gtgctttttg aaaggacggc gctggctgag tctcaggtgt
     1201 attcaggggt gattgatatg cctgcaggaa gggggcatat gacgctggag ttttctgtgt
     1261 ctgcctggct ggtgaatgga tggtacccga cagcgagtat cagcgatctg ctggttgtcg
     1321 tgatgaagaa gtccacggcg ggtatcagta tttcgtgatc atgaggagcc ggattgccgg
     1381 aaggagacaa aaaccgtaca ttatgcgcgg gtgcctttgg ctgatggccg gagggagcac
     1441 ctgaaggccg gatgtgaaaa ggccccggac aaacattcat gtttaacccg aggcctgacg
     1501 ttcctacctt caacaagt
//
LOCUS       DAZGHF010000338         1501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGHF010000338 DAZGHF010000000
VERSION     DAZGHF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.341"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1207)
                     /locus_tag="ACHK3A_005188"
     CDS             complement(<1..1207)
                     /locus_tag="ACHK3A_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFO8743113.1"
                     /translation="MAKVKVKTLIGSVVTVAFFLTVIVAFLFYAFPENVASATGFGPY
                     DGLRILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESITPVTNTIPGEE
                     DDDEPVFSEDAVSEHLRFRYGRRWQRKVRLLLVMGDPDVVRKAAPGLCHDLWQEGDGY
                     VLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALLTDGERDALLRYRQKAD
                     HQLGWQAPVWLWLTDNATGARQDAKAAATGVIFGPEGTVNGADEAFSTLALHLQKAGM
                     AQILNEPADFGLLQLSLRLRQELKASLAVLLSGLMQGTAAWRLRGVMFSPELTVAGAV
                     PNTCPDTPAWKAIIDDCDAVSGRKLGFNWLKMLRLILLSLIVLWGAGTLLSLLVNRTQ
                     IYQAQETAGQ"
     gene            complement(1200..>1501)
                     /locus_tag="ACHK3A_005189"
     CDS             complement(1200..>1501)
                     /locus_tag="ACHK3A_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743114.1"
                     /translation="NELKGSWISGPTLTSGSSWNNACEENGVTFSLSEDNFSIDPVLG
                     YTGHAAPWLAITLADAAFEQRGAQVIAAQPTADKDDVWVAVITKEEVRKESPGNG"
BASE COUNT          348 a          441 c          394 g          318 t
ORIGIN      
        1 cctgtcccgc cgtttcctgc gcctgataaa tctgagtacg gttaacgagc agagacagca
       61 gtgtaccggc cccccacagg acaatcaggg aaagcagaat caatcgcagc atcttcagcc
      121 agttaaagcc cagcttcctg ccgctgacgg catcacagtc atcaataata gctttccagg
      181 caggtgtgtc ggggcatgtg tttggtaccg ctccggcaac ggtcagttca ggactgaaca
      241 tgacaccgcg taaacgccag gcagcagtcc cctgcatcag tccactgagc aaaacagcca
      301 ggctggcttt cagctcctgc cgcagtctga gtgacaactg aaggagaccg aaatcagcag
      361 gttcattcag tatctgcgcc attcctgctt tttgcaggtg taacgccagt gtggaaaacg
      421 cttcgtccgc accgttcacg gtaccttccg gtccgaagat gacccctgtt gcggccgctt
      481 tcgcgtcctg ccgggcaccg gtagcgttgt cagtcagcca cagccagact ggtgcctgcc
      541 agcccaactg atggtcggct ttctgacggt aacgcaggag ggcatcccgt tcgccatccg
      601 tcaggagggc tgaaatactc atcacctgaa tgatgccatc aaccggttga ccggatcgca
      661 gacgcttcag ttttgaaagg aagatctcgt ccgggagtga ctgcgcgtct ccgccataaa
      721 tcagcacgta gccgtcgcct tcctgccaga ggtcatggca aaggcccggg gctgctttcc
      781 ggaccacatc cggatccccc atcaccagca ggagtcgcac cttacgctgc cagcgacgcc
      841 catagcgaaa gcgcaaatgt tctgaaacag catcttcgct aaaaaccggc tcatcgtcgt
      901 cttcttcacc gggaatggtg ttcgtcaccg gagtgatact ctcagccttt ttctgccccc
      961 actggaaata cagcccggat ttaccggcga agtcgaaagc cttctccgtc agccagccga
     1021 gtaaaaacag cgtggcaacc agtatggcgc agaatgtgag tatacgcagc ccgtcatacg
     1081 gaccgaagcc ggtggccgaa gcgacgtttt ccggaaaggc ataaaaaaga aatgccacga
     1141 tgaccgtcag aaaaaaggcg acagtcacaa cagagccgat gagtgtttta acttttacct
     1201 tagccattcc cgggtgattc cttgcgaact tcttctttag tgatgacggc aacccagacg
     1261 tcgtctttgt cagctgtggg ctgggcggcg ataacctgcg cacctcgttg ttcaaaggca
     1321 gcatcagcaa gcgtgatggc aagccatgga gcagcatggc cggtatagcc cagcacggga
     1381 tcgatactga aattgtcttc tgaaaggctg aatgtcactc cgttctcttc gcaggcatta
     1441 ttccagctgc tgcccgacgt caatgtggga ccggagatcc agcttccttt aagctcattg
     1501 g
//
LOCUS       DAZGHF010000339         1496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGHF010000339 DAZGHF010000000
VERSION     DAZGHF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.342"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..298)
                     /locus_tag="ACHK3A_005190"
     CDS             complement(<1..298)
                     /locus_tag="ACHK3A_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO8743115.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVI"
     gene            complement(301..1005)
                     /locus_tag="ACHK3A_005191"
     CDS             complement(301..1005)
                     /locus_tag="ACHK3A_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO8743116.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
     gene            1051..>1496
                     /locus_tag="ACHK3A_005192"
     CDS             1051..>1496
                     /locus_tag="ACHK3A_005192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743117.1"
                     /translation="MRITDNKKTNQAEYTFNADYFLIAVDTGNTAHFLTSVATGSTVR
                     FLTSAATGSTVHFLTSAATGSTVRFLTSAATGSTVRFLTSAATGSTAHFLISAATGST
                     VHFLTSSATGSTVRFLTSAATGSTVHFLTSAATGSTAHFLIPAATG"
BASE COUNT          345 a          431 c          296 g          424 t
ORIGIN      
        1 caataaccac ttcagaagcg cgttttaaca gcccccggat cacaataagt tgttttttca
       61 ccttcggctt caccaccatc tgccattgtt ccggtatcag cggcaacaca tcagaacgcc
      121 atggggaacc gaactgttca ccgtaagctt ccggctcagc cagttccagc aggtgtccta
      181 tcgcccaggt gacagcaata ttgtcatctc cgatgtatcc gtcgccccgc ttactgagcg
      241 ccagttcacg ggcgatatct ttcgcctgag aaggtttttc acacagaaag agttgcatac
      301 tcaccccgct ttaaccggtg gtgaataaac agaacgtttc ttaccgctca tcacgtcagg
      361 atccggctcc cccatgcggt taaccgcctc ctttccacgg gctgtgccgg acaaaatatc
      421 agcacgtgta acactgactg atttataatt ttgaataatc ccgtaaatcc tgcgtaccgc
      481 ataccctccc tgattcaaca actcatcccg ttttgtgcgg gaaataagtc cgtaatgaaa
      541 tacctgaaat accttcatga ccagtcgatc atatccgatc agaagccata cacatcgata
      601 accgaccgga gaactactgt acacgccaat atttaccggc gaagaggatt taatatcaga
      661 aagggagatc tgtcccggca ggtttttaag gatcccgtca agccacgcaa gatgcttttc
      721 aattgtttca gttgccttcg tcagggcatt ttccagcctg actaacatca tatctgcata
      781 aggattatcc ctggctgaat ctctggttgc ctgacccgca aacgcaatag ccctgggcat
      841 actgatgatg tcaggccgct tttcctgttt ttcctccttc tgattattcc tgcgtccctc
      901 ccagagacgg atcgcatatc gggtatgaag ggcaatagtc agcgatgact gcagagcacc
      961 aacttgttct gattttctga cttctccggt tttctcctgg ggcattgtgt ttactccaaa
     1021 tgatttcatg accagaagat agtccgcagg ttgcgtatta ccgacaacaa aaaaacaaat
     1081 caggcagaat atacatttaa cgccgattat tttttaatcg ctgttgacac tggcaacacc
     1141 gctcactttt taacctctgt tgccactggc agcactgttc gctttttaac ctctgctgcc
     1201 actggcagca ctgttcactt tttaacctct gctgccactg gcagcactgt tcgcttttta
     1261 acctctgctg ccactggcag cactgttcgc tttttaacct ctgctgccac tggcagcact
     1321 gctcattttt taatctctgc tgccactggc agcactgttc actttttaac ctcttctgcc
     1381 actggcagca ctgttcgctt tttaacctct gctgccactg gcagcactgt tcactttttg
     1441 acctctgctg ccactggcag cactgctcat tttttaatcc ctgctgccac tggcag
//
LOCUS       DAZGHF010000340         1486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGHF010000340 DAZGHF010000000
VERSION     DAZGHF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.343"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1486
                     /locus_tag="ACHK3A_005193"
     CDS             <1..>1486
                     /locus_tag="ACHK3A_005193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743118.1"
                     /translation="LFSGTGENNTQVMIKYKTERGEEKNIKVVVDDTGKYEINLNGWD
                     IKDADGAEVKIVDSAGNVKSGNLYNIARLYVDMNTNKAITLDSAYNIIGSQKEGTDPA
                     KALIMSDDNDWVYIGGGISPHIGDQNPGSDNNIDMAGGDDILSSVGAVLDGANINMGD
                     GDDKLYTQDGFASSGTRNIIMGDGNDVISVDNSFGGKNTISLGEGNNLFIVGNYVNSI
                     AENDITAGSGDDRIEIGTNLDGKNKIDLGDGDNTIQVGGYITNSHTITGNSGDDIIYV
                     ATNIDGSGSFNLGEGNNNFIVGGYIQGKNTIEMGSGDDTVSVSTRIADNVKIQLNAGD
                     DSVYAGGLLNKAIVDLGDGDDVVTLSGISDNGKRNNMEELVSTNAMLTGGEGNDTLKI
                     NGSFKLLNMKNISGFETIDLGESSENHLDVGIKSDMLDISSSSGVKIFTIMGGAGNTV
                     DLGKANITSHNSVEQGNYADSWYKGDTVDGYTTYTPVGDKSVEL"
BASE COUNT          488 a          195 c          366 g          437 t
ORIGIN      
        1 tattattttc cggtacaggt gaaaataata cgcaagtgat gattaaatat aaaacagaac
       61 gtggggagga aaaaaatata aaagtagttg ttgatgacac aggaaagtat gaaataaatc
      121 taaatggctg ggacatcaag gatgcagatg gcgctgaagt aaaaattgtt gattctgctg
      181 gtaacgttaa atcaggaaac ttatataata tcgcgcgttt gtatgtggat atgaatacaa
      241 acaaagctat aacgctggat tcggcttata atataattgg ctctcagaaa gaaggaaccg
      301 atcctgctaa agctcttatc atgtcagatg ataatgactg ggtgtatatt ggtggaggga
      361 tatctcccca tataggggat cagaatccag gctctgacaa caacattgat atggctggcg
      421 gtgatgatat tctgtcatct gttggtgcag ttttggatgg agccaatatt aatatgggtg
      481 atggagatga taaactatat acgcaggatg gtttcgcttc ttcaggtacc cgaaatatta
      541 ttatgggaga cgggaatgac gttatttctg ttgataattc atttggtgga aaaaatacca
      601 tttctttagg agagggaaac aacttattta ttgttggtaa ctatgtaaat agtattgctg
      661 aaaatgatat tacggctggt agtggtgatg acagaataga aattggtaca aatctggatg
      721 gtaaaaacaa aattgatctt ggggatggtg acaacactat tcaggttgga ggatacataa
      781 ccaactcaca taccattact gggaatagtg gtgatgatat tatctatgtc gctacaaata
      841 tagatggttc tggctcattt aatttaggtg aaggtaataa caactttata gtcggaggat
      901 atatccaggg gaaaaacaca attgagatgg gttccggtga tgatacggtt tctgtttcca
      961 ctcgtattgc agataatgtt aaaatccaac ttaatgccgg ggatgacagt gtgtatgcag
     1021 gtggattgtt aaacaaggca attgttgacc tcggggatgg ggatgatgtc gtcacattga
     1081 gtggaatatc agataatggt aagcgtaata atatggagga gttagtatcc actaatgcaa
     1141 tgcttacagg aggggaagga aacgatactc tgaagattaa tggttctttc aaattgttaa
     1201 atatgaaaaa tatatctggg tttgaaacta ttgatcttgg ggaaagttca gagaatcatc
     1261 tggatgtcgg tataaaatcg gatatgctgg atatttcatc gtcatctggg gtgaaaattt
     1321 ttactatcat gggtggggcg ggaaatacag ttgatttagg taaggcaaat ataacgtctc
     1381 acaattcagt agaacaagga aattatgctg attcatggta taaaggagat actgtggacg
     1441 gatacacaac gtatactcct gtaggtgata aatctgttga actcca
//
LOCUS       DAZGHF010000341         1480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGHF010000341 DAZGHF010000000
VERSION     DAZGHF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.344"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..828
                     /locus_tag="ACHK3A_005194"
     CDS             <1..828
                     /locus_tag="ACHK3A_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743119.1"
                     /translation="CAQLKNILDVMIEEDDKGKACFTVNRIRSQDDRVTFIIEQKIKP
                     SESLLDKPWSLLEPKSFLTEKKHDSIDKNDASVSVKKVGLSEGDALKNTGVDKQEDDY
                     SRHVGTEESKSKVSGTEQNKNKGKTPPKVDRNVLVPSYVKFNFRGHKKCSRIFNELKS
                     HLTFDNTPNAISILLRIFIDLSVSTFVEENKLQFKEPHRTPGLHDKVKMCANFLRENK
                     KMTGSQCTAVITFSSQITKHDGSLQQYVHNPHLIPSKEAVNTEWDNFELLLSLIWSE"
     gene            898..>1480
                     /locus_tag="ACHK3A_005195"
     CDS             898..>1480
                     /locus_tag="ACHK3A_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO8743120.1"
                     /translation="MKFYTPLRYPGGKGKLSYFLKDVIEKNSLNDGAYAEPYAGGAGV
                     ALELLLEEYVRKIYINDADFAVYSFWSSVVSDTDELCRLISRAKIDMDEWSFHRYVIS
                     NPSEFTKLEVGFAAFFLNRTNRSGILKAGVIGGKSQNGKWKMDVRFNRADLISRIEKI
                     ANYNQRIVVTNLDALDFLDALNFMDNMGKVDKNK"
BASE COUNT          501 a          200 c          321 g          458 t
ORIGIN      
        1 tgtgcacaat taaaaaatat tttagatgta atgattgaag aggatgataa aggtaaggct
       61 tgttttactg ttaataggat tcgtagccaa gatgacaggg ttacatttat tattgaacaa
      121 aaaataaaac cttcagaatc tcttttagat aagccgtgga gtttactgga gcctaaatct
      181 tttcttacag agaaaaaaca tgattctatt gacaaaaatg atgcatctgt gagtgtaaag
      241 aaagtaggtc tttcagaagg tgatgcctta aagaatacag gtgtggataa acaggaagat
      301 gattattcta gacatgtagg cacagaagag agtaaatcta aagtttcggg tacggaacag
      361 aataaaaaca agggaaaaac tcctcctaaa gtagatcgta atgtgttggt gccatcttat
      421 gtgaaattta attttagagg gcataagaaa tgctctagaa tatttaatga gcttaagtcg
      481 catctgacat ttgataacac ccctaatgct atctcaatat tgttgagaat atttatagat
      541 ctctctgtaa gcacttttgt tgaagaaaac aagttacaat ttaaggaacc acatagaact
      601 ccagggcttc atgataaggt taaaatgtgt gctaatttcc ttcgggaaaa taaaaagatg
      661 acggggagtc aatgtacggc tgtaataact ttctcgagcc aaataactaa acatgatggt
      721 tcattgcagc agtatgtaca taacccacac ttaattccct caaaagaggc tgtcaacact
      781 gagtgggata actttgagtt gttattaagc cttatttgga gtgagtgaga tttgtctaca
      841 tctggacatg gatgtccaga aactgataaa ctacaaaatc aggaatgaga gggatttatg
      901 aaattttata ctccattaag ataccccggc gggaaaggta agttatctta ctttttaaaa
      961 gatgttattg aaaaaaactc gttgaatgat ggagcatatg cagaacctta cgctggtggt
     1021 gcaggggtgg ctttagaatt attattggaa gagtatgtcc gtaaaatcta tataaatgat
     1081 gcagattttg ctgtttattc tttttggtca tctgttgtta gtgatactga tgagctttgc
     1141 cgacttatta gccgagcaaa gatagatatg gatgaatggt cttttcatcg ctatgttatt
     1201 tcgaatcctt ctgagtttac taagcttgag gttggctttg ctgcgttttt cttaaataga
     1261 acaaatcgtt caggtattct caaggcgggg gttataggcg ggaaatcaca aaatggaaaa
     1321 tggaaaatgg atgttcgctt taacagggcg gatttaattt caagaattga gaagatagca
     1381 aattataatc aaagaatagt agtaactaat ctggatgctt tggattttct tgatgccttg
     1441 aattttatgg ataatatggg aaaggtggat aaaaataaaa
//
LOCUS       DAZGHF010000342         1473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGHF010000342 DAZGHF010000000
VERSION     DAZGHF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.345"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1276
                     /locus_tag="ACHK3A_005196"
     CDS             <1..1276
                     /locus_tag="ACHK3A_005196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743121.1"
                     /translation="PINNRMEIINYLIQNEKIKVLAKSIAENGTSPLETLAVMENENG
                     HFIALEGNRRVCALTLLNDPDLCPENERAYFRKLSDNSDVIPDAVECVLFENREDANK
                     WIELRHNGEQDGAGLLTWNATQKARHFGSSDNTLALKLLDYAETKGFISKQKRDGILT
                     TASRFLGNPVFRSTMGIRTGRSESNVMIDVSRDEFDKVLERFINDLINGENNVTSRSK
                     KEDWENYAKQLRSEGIAPKGHCEPFALFSTEHSNASGKGSTISKIVKNNQSPNNRKYI
                     IPSDFKVNIPDPILKRVFDELRTLECSEYTLASSVVCRAFLENIYSLAYEKFYGNYPE
                     GMAEHVVMEKIINKIEEDKNSLTKKEKKALEALKRVKTDTTNPLSPKTLGANAHAGFY
                     PDPTALKVHWDNISHILLFMLNKIFNSSSVHK"
BASE COUNT          525 a          239 c          296 g          413 t
ORIGIN      
        1 tcctattaat aaccgcatgg agataattaa ctatctgatt caaaatgaaa aaattaaagt
       61 attggctaaa agtattgctg agaatggaac aagtccatta gaaacacttg cggtgatgga
      121 gaacgaaaat ggacacttta ttgcccttga ggggaatagg agagtttgtg cattaactct
      181 gcttaatgat cctgatttat gtccagaaaa tgagagggct tatttcagaa aactatctga
      241 taatagtgac gtcattcctg atgcagttga atgcgtttta tttgaaaata gagaggatgc
      301 aaataaatgg attgaattgc gccataatgg agaacaagac ggtgcggggc ttcttacttg
      361 gaatgcaact caaaaagcaa gacatttcgg ttcttctgat aatacattag cgttaaaatt
      421 attagattat gctgaaacta aaggatttat ttcgaagcaa aaacgagatg gcattttgac
      481 aacagcatcg agattcttag gtaatcctgt attcaggtca actatgggaa ttagaacagg
      541 gcgttcagaa tctaatgtga tgattgatgt ttccagagat gaatttgata aagttcttga
      601 aaggttcatc aatgacttaa ttaatggtga aaataatgtc acatcaagat caaagaaaga
      661 agactgggag aattatgcaa aacagctaag atcggaaggt attgccccca aaggacactg
      721 tgaaccattt gctcttttta gtacggaaca tagcaatgct tctggtaagg gaagtactat
      781 aagtaaaata gttaaaaata accagagccc caataataga aaatatatta ttccttctga
      841 ttttaaagtt aatatacctg atcctatatt aaaaagagta tttgatgagc tcagaacact
      901 tgaatgttca gaatacacat tggcttcttc agttgtatgt agggcttttc tggaaaacat
      961 ctattcatta gcatacgaaa aattttatgg aaactatcct gaagggatgg ctgaacatgt
     1021 agtaatggag aaaataataa ataagataga agaagacaag aatagcctca ctaaaaaaga
     1081 aaagaaagct ctggaggctc tgaaaagggt taaaactgat acgaccaatc cactatctcc
     1141 aaaaacgcta ggagcaaatg cccatgcagg cttctaccca gatccaacgg cgttaaaagt
     1201 ccactgggat aacatttcac atatcttatt gtttatgttg aataaaatat tcaacagtag
     1261 ctctgtccat aagtaatcaa ctgttctaaa ataaatcggt atgaatctat tttcacaggg
     1321 aaagactatg tcagcaactc catcaccatt acgatatccg ggagggaaag ccgcaattca
     1381 agatatgatt tcggctataa tttgctccaa caaccttact ggcggacact atgcagaacc
     1441 ctacgctgga ggtggtggtc ttgctttatc att
//
LOCUS       DAZGHF010000343         1453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGHF010000343 DAZGHF010000000
VERSION     DAZGHF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.346"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHK3A_005197"
     CDS             complement(<1..1001)
                     /locus_tag="ACHK3A_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO8743122.1"
                     /translation="MNRSLTSKAGSVVQQLSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQVPMSDGTPALPIRTVFGVVESFRRIF
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTTGDAESPETGAYFARLRHERILNAQYCVSGHSSSPYLTPGQVLETDG
                     "
     gene            complement(998..>1453)
                     /locus_tag="ACHK3A_005198"
     CDS             complement(998..>1453)
                     /locus_tag="ACHK3A_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO8743123.1"
                     /translation="SDLLMQQLSEKPETTESELHELIRPLLRDHFQPALLARFQTVIY
                     RPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERLYDALTSACLLPDTGARNVESL
                     LNQQLLPVLSRQLLSHMAAKQKPQALTLTWSDEDGIVMVFDAQAEGEPS"
BASE COUNT          321 a          425 c          385 g          322 t
ORIGIN      
        1 ccgtccgttt caagtacctg acccggcgtc agataagggc tggaggaatg cccactgaca
       61 caatactgcg cgttcagaat gcgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgt cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgcccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccagattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgttc aagacggctg
      421 tccatttcat agcgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagttcc ctaggtggat actcccggga gagccggaat
      541 tcaaaatcct gcccttccag accatgttta cgtaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcgat acggggaaca
      661 atcgtcagtg cgtaacgggt ctcatcattc gaggcaaaga tacgacgaaa cgactcaacc
      721 acgccgaata ccgtcctgat cggcagggcc ggtgttccgt cagacatcgg gacctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga agagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgattgccc tgaaagctgc
      961 tgtacaacgc tgccagcctt gctggtcagg gaacggttca tgacggctct ccctctgcct
     1021 gtgcatcaaa taccatcaca ataccgtcct cgtcactcca tgtcagagtc agcgcctgtg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagttgct
     1141 ggttcagcag actctccaca ttccgggcgc ccgtatccgg cagcaggcag gctgatgtca
     1201 gggcatcgta cagacgttca tcaaccgatg tgctcagccc ataatggcag tgcaggcgtt
     1261 cgcagacctg tgcaagcttc atctccacaa tggtgcgcat ggcagactgc gtcagcggac
     1321 ggtaaatcac ggtctggaaa cgggcaagca gtgctggctg gaagtggtcg cgcagcagtg
     1381 gacggataag ctcatgcagc tccgattccg ttgtctccgg cttttcgctc agttgctgca
     1441 tcagaaggtc act
//
LOCUS       DAZGHF010000344         1453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGHF010000344 DAZGHF010000000
VERSION     DAZGHF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.347"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHK3A_005199"
     CDS             complement(<1..1001)
                     /locus_tag="ACHK3A_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO8743124.1"
                     /translation="MRSFLTSKAGSVVQQFSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQTPVSDGTPALPLRTVFGVVESFRRIS
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTAGDAESPETGAYFARLRHERILNAQYLVSGHSSSPYLTPGQVLETDG
                     "
     gene            complement(986..>1453)
                     /locus_tag="ACHK3A_005200"
     CDS             complement(986..>1453)
                     /locus_tag="ACHK3A_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO8743125.1"
                     /translation="SDLLMQLLDEQPEATEASLHELLRPILRDHFQPALLARFRTVIY
                     RPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESL
                     LNQQLLPVLSRQLLSHMAAKQKPQALTLAWSDEDGIVLVFDAQAEGESSCAVS"
BASE COUNT          322 a          432 c          391 g          308 t
ORIGIN      
        1 ccgtccgttt caagtacctg acccggcgtc agataagggc tggaggaatg cccgctgaca
       61 agatactgcg cattcagaat acgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgc cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgtccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccagattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgctc aagacggctg
      421 tccatttcat aacgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagctcc cggggtggat actcccggga gagccggaac
      541 tcaaaatcct gcccttccag accatgttta cgcaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcaat acggggaacg
      661 atcgtcagtg cgtaacgggt ctcatcattc gaggcagaaa tgcgacgaaa cgactcaacc
      721 acgccgaata ccgttctgag cggcagggcc ggtgttccgt cagacaccgg ggtctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga ggagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgattgccc tgaaaactgc
      961 tgtacaacgc tgccagcctt gctggtcagg aaactgcgca tgacgactct ccctctgcct
     1021 gtgcatcaaa taccagtaca atgccatcct cgtcactcca tgccagagtc agcgcctgcg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagctgct
     1141 ggttcagcag actctccaca ttccgggcac ccgtatccgg cagcaggcag gctgatgtca
     1201 gggcatcgta cacacgttca tcaaccgatg tgctcagccc ataatggcag tgcaggcgtt
     1261 cgcagacctg tgcaagcttc atctccacaa tggtgcgcat ggcagactgc gtcagcggac
     1321 ggtaaatcac ggtccggaaa cgggcgagca gtgcaggctg gaagtggtcg cgcagtatcg
     1381 ggcgcagcag ttcgtgcagg ctggcctcag tggcttccgg ttgttcgtcc agcaactgca
     1441 tcagaaggtc act
//
LOCUS       DAZGHF010000345         1441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGHF010000345 DAZGHF010000000
VERSION     DAZGHF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.348"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1285)
                     /locus_tag="ACHK3A_005201"
     CDS             complement(<1..1285)
                     /locus_tag="ACHK3A_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8743126.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPNTDY
                     SVKLLWAWSTYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAFMDYQPGV
                     EQTPQALWGICLLMTIIPAILNLITGLIMRFYLINN"
BASE COUNT          498 a          328 c          257 g          358 t
ORIGIN      
        1 cattattaat cagataaaat ctcatgatga gtccggtaat caggttaagt atcgcgggaa
       61 tgatcgtcat taacaggcag atcccccaca acgcctgtgg tgtttgttct acgccaggtt
      121 gatagtccat aaacgctaaa gcgattccga taaaccctcc cgccagtccc ataccaaact
      181 tttgaaaaaa gagaatcccg ccaaatgcca gtccagaaac acgttgcccc gttttcattt
      241 caccataatc cactgactca gcaatggatg cccaataaat gggaagctga atatcagtca
      301 gaaaggtcac cacaatataa aatgtaaaag ctaacactat agaatttggt tgcacgaaat
      361 acatcgcacc acccaccacg aacgccagta attgggtata acgaaatatt tttattttgt
      421 cgtagagtcg agtaagccag gtgcaagcga tcatagccag aacagaggcg atcacgcctg
      481 aagttaaaaa cggtgaaatt aaagcatccc caccattcag gtaatatttt gcataatatg
      541 cagcgacaga gttacgaata atcccgccaa acataatgat cgcaattaca gcgcccaaaa
      601 tgagccattg atcatttttg atcagtaacc gaaactgcgc ggccataccg agactgggtt
      661 ttggatggta gatgcgctct ctcacgctaa aaaaacaata caggcataaa cacgttgcca
      721 gaattcccat tgcccccatt gctatttgat aacccagttg tacgttattc ttcccaagat
      781 acattgcaga taaaggaaca ataattgtta cggcgaacat ggcaattttt gtcataacaa
      841 aacgataacc attggcgctc aatcgctctt gtggatcatc cgtgataacg ctgattaatg
      901 aaacataagg aatagcgatt aacgtatagg tcaaggtaag taacacatag gtagaccagg
      961 cccataaaag cttaactgaa taatcggtat taggtgtggt gaacatcaac caaatgctta
     1021 tcccaaacgg tattgacata aaaagcaacc acggccgata ttttccccaa cgtgtattcg
     1081 ttctgtctgt cattgttccc acaactggat caatcacagc atcaaggata cgtacagaaa
     1141 ataacagaat acctaagtcg acgggtttca gaccaaatac atccgtgtaa aaataagtga
     1201 tgatcatggc caaagacatc attactatcg cgatagccat atcaccagca ccaaaaccta
     1261 tcttttcatt tattgtaagt ttcatacaca gcactcattg attacgtgaa cttatattaa
     1321 aaacaaatat atctgaactc attcagcaca taactttacg agtgagcata aatgcattgg
     1381 ttgataccac aacaaaccat tgagtcaata ttgagcaaca tcacataaaa accaatttca
     1441 c
//
LOCUS       DAZGHF010000346         1439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGHF010000346 DAZGHF010000000
VERSION     DAZGHF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.349"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..625
                     /locus_tag="ACHK3A_005202"
     CDS             <1..625
                     /locus_tag="ACHK3A_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743127.1"
                     /translation="QINSQIENNKTSINEVNMLFGDKKQEGRSVIKKTFPNGTYNIVS
                     YQGHLNDLGGTYAHRVLFVVYDKDGVVINHDIAINNFREKNKFEQRPDELRLTAFNEL
                     NKNDSKSKVLNLLGEPRTLSFSDSGNVLWVYSNTEISRDASSYIPVFNMIKGTESGMS
                     ERVYIELKDNKVENIYLASFKIVQGRGATNAADYKEDVIDIRKKYEL"
BASE COUNT          463 a          247 c          306 g          423 t
ORIGIN      
        1 gcaaataaac tcacaaattg aaaacaataa aacttcaata aatgaagtta atatgctatt
       61 tggtgataaa aaacaagaag gtagaagtgt aattaaaaag acgtttccca acggtactta
      121 taacatcgta tcctatcaag gacatttgaa tgatttaggt ggtacttatg ctcatcgtgt
      181 tctctttgtt gtttatgata aggatggtgt agttatcaac catgatattg caataaacaa
      241 cttcagagag aagaataaat ttgaacaaag accagatgag ctacgtttaa ccgcttttaa
      301 tgaattaaat aaaaatgata gcaaatcaaa ggtattaaat ctccttgggg agccaagaac
      361 tttatcattc tctgattctg gaaatgtttt gtgggtttac agcaatacag aaatatcaag
      421 agatgcatct tcctacattc cagtttttaa tatgataaaa ggtactgaat ctggaatgtc
      481 agagcgggtt tacattgagt tgaaagataa caaagttgaa aatatttatt tggcatcatt
      541 taaaatagtc cagggccgtg gggctacaaa tgcagcagat tataaagaag atgtaataga
      601 tatcaggaag aaatacgaat tatagatttt taccaagtaa aatataatcg tgttttttat
      661 acaaaaatta tttggccatt tgctctccct ggtctggcct tcatcagttc cgggggattt
      721 tttgcctgta atccccctgt agttggcatg gcagaggaca tggataatgg ttcccgtctg
      781 gcattttcgt gggcgttggt tgtaaaaaaa taccagcagg ataagcgcag attgatcagt
      841 gatgattgag cgcgaatctg acaacaaggt aagtacaacg acacagcaac aaaaaaagac
      901 gccagaaacg cgctcagacg ctttgtaacg cgattttttg cttcaggcaa gtatttaatt
      961 ttcaggccgc cataaacgtt ttaagcacgt ctccggtccc taatcaatga attcccggtc
     1021 attaactcgt gtttatccgg aaacgcgctc tgatgttata tcgtattgca ggaacgctgt
     1081 cgagggtgtc agggtggtgg cgtttgtctg attgtgcgtg cgcggtccgg cgaacacgca
     1141 tcctgtcccg tttttcgtac ggttttttat tgattaccga acagatgcat ccatattgcc
     1201 atgttgtgaa aaatacgtta aaacaattac gagcgtgctg ttgggctgat atatcaacat
     1261 caggccaata tgtattaacc cgactggctc tctcttttta actccgtaaa tataaatgcg
     1321 tgtatgaatc aggataaagc aaattattta gcaaataaat atttttcacc cgcaaacgaa
     1381 gcagtcaggc tggagcgtga aggtgattac attgaagcat ggaaaaaatg gagtgaagc
//
LOCUS       DAZGHF010000347         1431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGHF010000347 DAZGHF010000000
VERSION     DAZGHF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.350"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            49..1035
                     /locus_tag="ACHK3A_005203"
     CDS             49..1035
                     /locus_tag="ACHK3A_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO8743128.1"
                     /translation="MYRYDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            complement(1350..>1431)
                     /locus_tag="ACHK3A_005204"
     CDS             complement(1350..>1431)
                     /locus_tag="ACHK3A_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="subtilase cytotoxin subunit B"
                     /protein_id="HFO8743129.1"
                     /translation="LTSNALVGLSTCATSTECFGPDRKKN"
BASE COUNT          421 a          265 c          351 g          394 t
ORIGIN      
        1 ttaataatga aaatatttaa ttaaatctat tatgtaaagg ggggagtgat gtatcgttat
       61 gattttaatg aaaaaataga tttctggtat gtgatagccc tcgcaggaca gtctaatggt
      121 atggcctacg gtgaaggtat tcccctgcca gatacactcg acaaacccga gtcccgtgta
      181 aaacaactgg ctcgtcgaaa aacaatcaca ccaggaggaa aagagtgtaa attcaatgag
      241 attattccgg cagatcactg tctgcatgat gttcaggata tgagtggata tcaccatccg
      301 gctgctgatt tgcataaagg ggaatatggt tgtgtcggac agggattgca tatagctaaa
      361 aagttactgc catacatacc tgagcaagca gggatattac tggttccttg ttgcagagga
      421 ggtgccgcct ttactgttgg agctgaaggt atgtatgtac ctgatacagg ggcaacagct
      481 gatgcaatgc ggtggggcac cggaacagca ctctatgagg acttagtagc ccgggttaag
      541 gtagctctgg aatataacag aaagaataaa ctgctatctg tctgctggat gcagggggag
      601 tttgatctta tgtcaccaga ttatgaaaag catcctgact tgttttatca gatggtaact
      661 tctttcaggt ctgagttatc tgaatactca tctcagtgtg ttggtaattc gtcagaaaga
      721 gttccatggt tgtgtggtga tacaacctgg tactggaagg aatcttatca aaaagaatat
      781 gattttatat atggacatta cagacaacgg actgatgatg aaatacattt tctcagcttt
      841 caggatagta accgacatga attaactaat gagccagaag aagatgccga tgatttatct
      901 gtcgggtatc tggggtcatc atggaggaca gaattaagct ggacaacgtc acagagaagt
      961 acacacttta attcaatggc cagaagggga gttattgctg aatgttatgc gcaaaaaata
     1021 agaaattatt tgtagacaag ttcaggatat atctacagct ctgacatgat tctctgagag
     1081 acatgacgga gtggtatgga gttgtcggta tcaacaccaa gatagctggt cgttaccgtt
     1141 tgatatcact ggttctgata ctatgtggac ggtagtgtgt cacgaaggca cgcaccaggg
     1201 acgtgagtcc ctgtaatatc atcgacagtc ccattatggc atgtctgcac ctacaacgtc
     1261 atttcgaggt cggctcagtg ttcactcgag tcttatatca ctgacgccga cactccatcc
     1321 agagtatctc tggacggaaa catcacctat tagttctttt ttctgtcagg gccaaaacat
     1381 tcagtcgatg tggcgcaggt cgataaccca acaagggcat tggacgttaa t
//
LOCUS       DAZGHF010000348         1419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGHF010000348 DAZGHF010000000
VERSION     DAZGHF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.351"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            34..489
                     /gene="tssE"
                     /locus_tag="ACHK3A_005205"
     CDS             34..489
                     /gene="tssE"
                     /locus_tag="ACHK3A_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO8743130.1"
                     /translation="MNRKSPSLYEILYGNFTGGLELNQVSEEEQVILSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTSILQGMPGTAHQLMRVLSDVLLKYEPRIKRVGVTMQEQSQ
                     PGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRYLKQQQFVDNVMTFRG"
     gene            542..847
                     /locus_tag="ACHK3A_005206"
     CDS             542..847
                     /locus_tag="ACHK3A_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743131.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            902..>1419
                     /locus_tag="ACHK3A_005207"
     CDS             902..>1419
                     /locus_tag="ACHK3A_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFO8743132.1"
                     /translation="MTSEVKLKTGNDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTASWYTIARMHTTGLSGLNEGLALIVALTRHHWPVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDNLPLLYQTETCLKTLSDILTRHELKQSSKVALPE
                     AMVKGYITRLEN"
BASE COUNT          369 a          320 c          372 g          358 t
ORIGIN      
        1 taacatgaca gtaatacaaa ttggattatt attatgaacc gaaaatcccc atcattatat
       61 gaaattctct atggtaattt cactggcggg ctggagttaa atcaggtcag tgaggaagag
      121 caggttattc tttcggtgct cgataacatg cagcgtattc tgaatacccg ggccggaagc
      181 cttaaacatc ttccggatta tggtctgccg gatatgacaa gcattctgca gggaatgccg
      241 ggaacggcgc atcaactgat gcgtgtgctt tcagatgttc tgctgaagta tgagccacgt
      301 attaagcgtg tcggggtgac gatgcaggaa cagagtcagc ctggtgagtt gcattatgtc
      361 attgatgcag aacttaaaga tgcaggactg gttcgttacg gaacaacttt tatgccggag
      421 ggcagggtgt tactgcgata tctgaagcaa cagcagtttg tggataatgt gatgactttc
      481 agggggtgat ttatggttcc ccttatccgt gtgcttaaca gacataaacg agggctgaat
      541 gatgaacccg ttatttaatg acatccagat gcgccttttt tatctgaatc atgcaccgta
      601 ttcctggcac tggaatgtca gattcaggcc acaggaagcc gtatatattg gtaatgatgc
      661 ctgccatatt accattacct gtaatcagtc cggttttcat ttaaccaggg acgggcagcg
      721 actgttcact gaacgttata tcagaacact cagtgagtta ttggcggttc tgaagcggcg
      781 gtgggatgtg acaccggcaa tcatccgtgc ggtggaatac ctttcccggg taccggtatc
      841 acactgacgc tctttccggg aatattaatt atgatttgat acagcaacac tgaatctgcc
      901 gatgacttct gaagtaaaac tgaaaacagg taatgacccc cggtcattac cggattatgc
      961 tgccctgcgc gatgaaatca gcaagctgac tcacccggca cgtccggatg tggactggcg
     1021 ttatgtggag acgctctgcc tgcgtctgta tgaacacaat ggcgtggaat tacagacggc
     1081 atcctggtac accatcgccc ggatgcatac aacagggtta agtggcctga atgaagggct
     1141 ggctctgatt gtggcgctga cccggcatca ctggccagtg atgtggccgc tgaataccca
     1201 cgcccggctg gaaattatta ccggtctgtt cagtcgtctg caaaaaacgc tccgggccat
     1261 gccaccggat gagcgtgaca acctgcccct gctgtatcag acagaaacat gcctgaaaac
     1321 gctgagtgac atactgaccc ggcatgaact taaacagagc agtaaagtgg ctttaccgga
     1381 agccatggtg aagggatata tcacccgtct ggagaatca
//
LOCUS       DAZGHF010000349         1418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGHF010000349 DAZGHF010000000
VERSION     DAZGHF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.352"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(404..778)
                     /locus_tag="ACHK3A_005208"
     CDS             complement(404..778)
                     /locus_tag="ACHK3A_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743133.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
BASE COUNT          397 a          336 c          318 g          367 t
ORIGIN      
        1 ggaggatgac ctacgaaaat ttcgtaagtc ctaccccgca caaggattaa ataatcacta
       61 caagcgattg attaaactta attatcgcga atcgcgagaa ttagccccgc agaaacaagg
      121 aaagaagaat tttacgaaaa tttcgtaaaa tccccccaca tctcccacgg ttgcccgtta
      181 agtgcaagcg cacaggcacg acctcattaa ccagttacca gcgctcacgc tcaaatatcc
      241 acaggaacgg ggctaaatcg cattctggcg caatgttcac tgtcagggcg agtattcacc
      301 agaaaacgtc atacaggctt acagtcggct taaaattcga taggcggagg accggatggg
      361 aatcgggaga cctggacaaa aagtgcaggc ctccatgaca cggtcaggac agtttgtgtt
      421 taaccagaat atttagcgcc tgtattgggg cttcgtggag ctcccgcaga cattcagggt
      481 cagtaacatg ttcctggtat cttgttttct caagtaacaa gcggtgcata tgcagcatac
      541 gggcaagagc cgcgcacacc tcctcaaatg cggtatcccg cttaccgtca ctgtcagagc
      601 cacaaaacag cggtacaacc tccacgccgt cgctgtgggc ctttagccag gcatcaacca
      661 cggttaaatc atcggtgaag aaccacgcac tgttagcgct gaatcgcatt ttccaggctt
      721 ccggtactgg ttgcggggag tatgaatatg ctggtggtat cttgtcgtga gtatccatag
      781 gctatcgttc cgtcattctg ttattcacca cagcgctaaa aacgccatga aggaccagaa
      841 taccgattgc ctcgtttgct gtatgaggtg cttaccacac attgggtaca cagatgaatc
      901 aggcggaagt gtgattaccg gaaaaaaccc ctttcagttt gacgaaaaaa aaaccgcagt
      961 gtgataacac tacggcacgc caccaaagaa acataacaag gaatcagcaa tgagtaatac
     1021 ctgtaagcct tcggggtgat tgtaacacat gagcaatctt ttactggaag ctgtttttca
     1081 ctggatcacg tatcgttatg gggattccca tatcggagag catccaccag cttcatagcg
     1141 gataaccgga aaaattccgg taaatatttg cctgagtaag tcatcaagcg cctgctaaat
     1201 gtgtatagaa atgtgtatag gattgatttt agaatatgtg ggaatggctt tattgctggt
     1261 ggcatggctt gttgttctgt tcccttcgcc gctccaattt atcttcacat catctacctg
     1321 ccatttttgt tgtatctgat tttctatatt atcttaattc aagcgcttca gacttttcgc
     1381 aatcgccgta atacttcccc atttttttgt aaataaaa
//
LOCUS       DAZGHF010000350         1414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGHF010000350 DAZGHF010000000
VERSION     DAZGHF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.353"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1414
                     /locus_tag="ACHK3A_005209"
     CDS             <1..>1414
                     /locus_tag="ACHK3A_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8743134.1"
                     /translation="VRTLAPDLDISIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAE
                     DAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVE
                     SRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDSTRIQTV
                     YQPGSFTPLIRIETDVTELEKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEE
                     IRTDRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARKVHLYHCDHRGLPLALISEDGN
                     TVWSAEYDEWGNLLNEENPHHVYQSYRLPGQQHDEESGLYYNRNRYYNPLLGRYITQD
                     PIGLAGGWSLYAYPLNPVNGIDPLGLSPADVALMRKKEQLNHQRAWDILSDTYDDMKR
                     LNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNMFGMYGRKVKLSH
                     SEMIEDNKKDLAVNEHGLTCP"
BASE COUNT          364 a          334 c          452 g          264 t
ORIGIN      
        1 gtgcgcaccc tcgcaccgga cctggatatc agcatcccgt atgccaccga cccggcggga
       61 aaccggctgc cggacccgga gctgcacccg gacagtacac tcacagtgtg gccggataac
      121 cgcatcgcgg aggatgcgca ctatgtctac cgccacgatg aatacggcag gctgacggag
      181 aagacggacc gcatcccggc gggtgtgata cggacggacg acgagcggac ccaccactac
      241 cactacgaca gccagcaccg tctggtgttc cacacgcgga tacagcatgg cgagccactg
      301 gtcgagagcc gctacctcta cgacccgctg ggccgccgga cgggaaaacg ggtatggcgg
      361 cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggaggt gacgtggtac
      421 gggtgggacg gcgacaggct gacgacggtg cagactgaca gcacgcgtat ccagacggtg
      481 taccagccgg gaagcttcac accgctcatc cgcatcgaaa cagacgtgac cgaactggag
      541 aaagcgcagc ggcggagcct ggcggagaag ctccagcagg aagggagcga ggacggacac
      601 ggcgtggtgt tcccggctga actggtgcgg ctgctggaca ggctggagga agaaatccgg
      661 acagaccgcg tgagcagtga aagccgggcg tggcttgcgc agtgcgggct gacggtggag
      721 caactggaaa aacaggtgga gccggaatat acaccggcgc gaaaagttca tctttaccac
      781 tgcgaccacc ggggcctgcc gctggcgctt atcagcgaag acggcaatac ggtgtggagc
      841 gcggagtatg atgaatgggg aaacctgctt aatgaggaga acccgcatca tgtgtatcag
      901 tcataccgtc tgccggggca gcagcatgat gaggagtcgg ggctgtacta caaccggaac
      961 cggtactaca atccgttgct gggacgatat attactcagg acccgattgg gctggcgggg
     1021 ggatggagtc tgtatgcgta tccgctgaat ccggtgaatg gtattgatcc attaggatta
     1081 agtcctgcag atgtagcgtt aatgcgaaaa aaagaacaat taaaccatca aagagcatgg
     1141 gatatattat ctgatactta tgatgatatg aagagattaa atttaggtgg gactgatcaa
     1201 tttttccatt gtatggcatt ttgtcgagtg tctaaattaa atgacgctgg tgttagccga
     1261 tcagcgaaag ggctgggtta tgaaaaagag attagagatt acgggttaaa tatgttcggt
     1321 atgtacggca gaaaagtaaa gttatcccat tctgagatga ttgaagataa taaaaaagac
     1381 ttggctgtaa atgaacatgg gttgacatgt ccat
//
LOCUS       DAZGHF010000351         1408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGHF010000351 DAZGHF010000000
VERSION     DAZGHF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.354"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1090)
                     /locus_tag="ACHK3A_005210"
     CDS             complement(<1..1090)
                     /locus_tag="ACHK3A_005210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO8743135.1"
                     /translation="MLRHDWKTYQSFMMYMPTGSGKTGLAAFITAGLVSRGMRVLFVV
                     PYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIASADTLIRRDFPQNIDL
                     LIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRYYQRLLKPTTIGELIQ
                     RGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCGADLVGDIVDNWLCNG
                     RDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQLMISRFETGATKIIVS
                     VGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKESCLIFDHSGTVHRLGF
                     PENIEYDELPGKNDGMQVSSSRGNEIREE"
     gene            complement(1169..>1408)
                     /locus_tag="ACHK3A_005211"
     CDS             complement(1169..>1408)
                     /locus_tag="ACHK3A_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743136.1"
                     /translation="CFTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIR
                     ALPEGISGFVFLRDGKHPQRYIADGVDVIRATRFK"
BASE COUNT          359 a          387 c          320 g          342 t
ORIGIN      
        1 tttcttcccg aatttcgtta ccacgtgatg atgacacctg catcccgtcg tttttgccgg
       61 gcagttcgtc atactcaata ttttcaggaa atcccaggcg atgaaccgta ccggaatggt
      121 cgaaaatcag gcatgactcc ttgcctggcg ctgtacgcaa tcctctccca atcgcctgaa
      181 gccagcgaat ctctgatttt gtcgggcgtg cgtaaatgat gcaccggaca tcactgtcaa
      241 atcccgccac cagaacgcca acgctgacaa tgattttcgt cgctcccgtt tcaaaacgac
      301 tgatcattaa ctgacgctca tcatgtggcg tttcagcaac catcacctct gcgttaattc
      361 cggctttgtt gaactgcatg gtcacgaagc tggcatgcgt tttattgacg cagaacgcca
      421 ctgtcggtaa atcccttccg ttacacagcc agttatcgac aatatcgcca accagatccg
      481 cgccgcacat aatctctgcc agttgtgatt cgtcgtaatc cctgccgtac tccattgaga
      541 ctttcgtttt tacaccttcc agatccggtt tcgtgggcgc ataaaattcg taattgctca
      601 gttcgccacg ctggatcagt tcccctatag tggtcggctt gagaagacgc tggtaataac
      661 gccccaggaa cgatgaaaag ggtgtaccgg acaacccaat caccttcaca tttccctctg
      721 caaccagtcg ttcgatttcc gtgaggatgc ggcgcttacg gagatgcgct tcatcaataa
      781 tcagcaggtc gatattctga ggaaaatcac ggcgaatcag cgtatccgca ctggcaatct
      841 gaatcagtcg tgacgggtcg ttattgggat gatcacgcca gataaagctg atttcatccc
      901 cggaaagccc gtactgaaca aagcgctgtg ctgtctggtt gatcaaaatg gtataaggaa
      961 cgacaaacag aacgcgcatg ccacggctta ccagaccagc agtgatgaac gccgccaggc
     1021 cggttttacc actccctgta ggcatgtaca tcatgaagga ctgatatgtt ttccagtcat
     1081 gacgcaacat attcagcgca cgttcctgag caaaattcgg cttgatattc agcatggtat
     1141 tctcccgatg acagggctga tacagtgatt acttaaaccg ggtcgcccgg atgacatcca
     1201 caccatcggc gatataacgc tgtggatgtt tgccatcacg gagaaaaaca aatcccgaaa
     1261 tgccttcagg aagcgctcga attttcatca gtgaacgctg acggggcgat cggttatcca
     1321 cctcaactgc gcaacgctga ccatcgggag aaataaccag aagatcgata aatcccctgc
     1381 gatcaccttt cagcctgaca gtgaaaca
//
LOCUS       DAZGHF010000352         1394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGHF010000352 DAZGHF010000000
VERSION     DAZGHF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.355"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1394
                     /locus_tag="ACHK3A_005212"
     CDS             <1..>1394
                     /locus_tag="ACHK3A_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HFO8743137.1"
                     /translation="QNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELAQQAE
                     VRRQAGGNRDIPQPETIAPESETTVFTDRESTVRGGEVRGKKIEDFGEEIKGAAKHRY
                     AQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIPAKTK
                     HNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPPSQMD
                     RASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAKVWFAE
                     QEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASDARKY
                     IDSHRDELVRHVKDMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRGVQFG
                     NYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPG
                     EVAINLTKGNGPGAL"
BASE COUNT          376 a          330 c          413 g          275 t
ORIGIN      
        1 cagaacgcga cagcaccggt acgtgaaggg ctaccggctc ctgacattgc gcgtaatgtt
       61 cgtatgcctc agcctgaatc acttccccgc actgtacggg actcactgcc tgagctcgca
      121 cagcaggcag aagtacgccg acaggccgga ggaaatcgtg acatcccgca gcctgagaca
      181 atcgcacctg aatctgaaac aactgtcttt actgacaggg aatctaccgt gcgcggaggt
      241 gaagtcaggg gcaaaaaaat tgaagacttt ggcgaggaaa ttaaaggggc agccaaacac
      301 cgttatgcac agcttgctga aacactgggt aaaacgctgg aagacaggga ttatgccacg
      361 cagccgctga gcaaactgtt cccgaaaccg gactacgcaa aactggcgaa cgaaggtgcc
      421 gatgctgaca ctctggcaat gatagcgctg tatcgtagcg atattccggc gaagacgaaa
      481 cacaatacgg caggctgggg ggagagcgta aaaaaagtac gacacagtgt atcggaaatg
      541 ctgaacggaa cggtcagcgc gaaacgcctc gcagaatgga tggaaggcag aatgccctcc
      601 cgttacgcgg atacctggca actgttacgc actctgccac cctcacagat ggacagggct
      661 tctgcttatc gggtggtatc gggtgtgtat caggcggcag gagggaagcg ttacgatccg
      721 ccacagaaac tttattcact gcgcaataag gacaataagg ggagtaacct ctttttctcg
      781 gaaagcaggg atgaattact ggcaaaggcg aaagtctggt ttgcagagca ggaggaaaaa
      841 tcacaggcga aaggtgatga aaaaacagca ccgtcaccgg atgacaaaat ccgctttgac
      901 gtttaccgga atacccgcag tggcgatatt tttatcgctt acggtaaaaa caaaatgcgg
      961 gtgagaggtg gctttaagtc agccagtgat gcgcgtaagt acattgattc acatcgtgat
     1021 gagcttgttc gtcatgtgaa ggatatgcgg gagatttcgc gtgaggagca gcgtaacgcc
     1081 accaaccgcg accgtaccgg accagaacgc cgtaaagggg atgtttcacc ggagcagttc
     1141 agtgatgcgt ttggtttccg tggtgtgcag tttggtaact acgtggaagg tccgcgtcgt
     1201 caggctgatt tgaaccgggc ttatgactcg ctgcatgacc ttgcggaagt actgaatgta
     1261 ccgacaaaag cgctttccct gaacggtcgt cttggcctgg catttggtgc ccgtggtaag
     1321 ggtaaggcgg cggcacacta tgagccgggt gaggtggcaa tcaacctgac aaaaggtaac
     1381 ggaccgggtg cgct
//
LOCUS       DAZGHF010000353         1379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGHF010000353 DAZGHF010000000
VERSION     DAZGHF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.356"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..534
                     /locus_tag="ACHK3A_005213"
     CDS             <1..534
                     /locus_tag="ACHK3A_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8743138.1"
                     /translation="IGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
     gene            531..1100
                     /locus_tag="ACHK3A_005214"
     CDS             531..1100
                     /locus_tag="ACHK3A_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743139.1"
                     /translation="MKRILFFLLMIFVSFGVIADCETQAKDQDCFTIFTKGTIFSAFP
                     VLNNKTIWRWYQNEDIGEYYWQTELGICKNNKFTPSGARLLIRVGSLRLNENHATKGT
                     LQELLNTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYECLTKVQHELLRSEEK"
BASE COUNT          480 a          242 c          283 g          374 t
ORIGIN      
        1 attgggctga aggggggatg gaatttttat cagtatccgt tgaatccggt tcagtatata
       61 gattcaatgg gactggcatc aaaatatgga cacttaaata atggcggata tggagcgaga
      121 cccaacaaac cgcctacgcc cgatccaagt aaattgccag acatagcgaa acaattaaga
      181 ctgccatatc ctattgacca ggccagtagt gcgcctaatg ttttcaaaac attcttcaga
      241 gcattaagcc cttacgacta cacactgtat tgcaggaagt gggtaaaacc aaatctgact
      301 tgtacgccac aggatgatcc ccagtatcca gggatggata caaagacagc aagtgattac
      361 ctgccacaga caaattggcc aacaactcaa ttaccaccag gatatacttg tgcagaaccc
      421 tatttattcc cagacattaa taaacccgat gggccagcaa cagcagggat agatgatttg
      481 ggtgaaattt tagctaaaat gaaacagaga acatcgagag gaataagaaa atgaaaagaa
      541 ttttgttctt tttgctgatg atatttgtta gttttggtgt tatagctgat tgcgaaacac
      601 aagctaaaga tcaagattgc tttaccattt tcactaaggg tactattttt tccgcgtttc
      661 ctgtcttaaa taataaaacc atatggagat ggtatcagaa tgaggacata ggtgagtatt
      721 attggcaaac agaacttggt atatgtaaaa ataacaaatt cacaccaagc ggggcgagac
      781 tcttaattcg cgtcggatca ctacgcctta atgaaaatca tgcaaccaaa ggaacgttgc
      841 aggagttgct aaatacagca gaaaaaacgg cgtttctcgg cgatcggttt agaagttata
      901 taagagcggg tatctatcaa aaaaagagca gtgatccagc tcagttgttg gcagtccttg
      961 ataattcaat catggttaaa tattttaaag atgagaagcc aacttatgct cgaatgacag
     1021 cacatctccc gaacaaagat gagtcttacg aatgtttaac aaaagtacag catgaactgc
     1081 ttcgtagtga agaaaaataa aatctcttcc tgagaaaata aattaactat tcgaaaaaat
     1141 atgttcttaa tgggtatcca ggtacaccag aaggtcgatt tggaaaaatg gattccgatg
     1201 gtaagaatat catatattct gaatgtaatg ttttacccac aacttttgag attcgatagg
     1261 tgtagagcat gagaaactca ctaatattac ttattatgat attgtttaca atatcacact
     1321 cagtatttgg aaaagaaaat tcagtggaaa ttttagtaaa tagtcgtgaa atggctttg
//
LOCUS       DAZGHF010000354         1359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGHF010000354 DAZGHF010000000
VERSION     DAZGHF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.357"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(72..299)
                     /gene="mchB"
                     /locus_tag="ACHK3A_005215"
     CDS             complement(72..299)
                     /gene="mchB"
                     /locus_tag="ACHK3A_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFO8743140.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALARIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(327..536)
                     /gene="mchI"
                     /locus_tag="ACHK3A_005216"
     CDS             complement(327..536)
                     /gene="mchI"
                     /locus_tag="ACHK3A_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFO8743141.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
BASE COUNT          473 a          274 c          246 g          366 t
ORIGIN      
        1 tgttttaaac aaaaaggata tattttacag gtactaccga aaaagtgcct gtaaaataaa
       61 taaaaatata attagctacc gccaccagca gaagaactgg cactaccact tcccacggtc
      121 gagccaattg ctgttgtcaa accggcagat acggctccaa ctacaacccc aagtggacca
      181 ccaggtattc tggcgagagc tccaacaata gctgcagcac ctgcagcatt agctgaagtc
      241 gctggcgcac ctcccgcccc ggaaatatat cttaactgtg attctgttat ttctcgcata
      301 taaactgcat ataaactcca tcatatttaa cttccgtttt tccagcctga ccagaccaga
      361 atccttcgaa tcccatgaat cagctcaata aaaccaagaa atattataaa gcttagaaaa
      421 acgtcaacat aagaattccc ttcgccaaca atccgaaggg atgaaagtga aaccaataag
      481 atagtaatag gtaaactgaa tatagccgtc aattgggaca gttttttata actcattaat
      541 ccctacctgt tgctttattt tcattatgaa aagttaccaa atcttagcac ctcagtcagg
      601 agagtcaggt catcgataaa gaatattcca gtagagtggc gctcatgatc aaagataaca
      661 gcaaagttga tgacagcgct gaccgactgt catttacaga aaccctgttt gtagcacatt
      721 ccatcttacc tgacccagac aaagacaaag acaaaagaga atgattgtta tttacttttc
      781 aaaaaaaagt gtcatattgt ccatataaag tcaagaggta ttttacgtta tgtcactgat
      841 gcaattgtta gtcaggatct catggttgtc gctcatgtat aacctgagac ttgaacgcac
      901 actaatagca ctgacggtca tacatcggga aatgtgagga ggcataagaa tgaattgtga
      961 taataatcac agaaatgaag aattcattgt tacctttgat aaaggcaaca agcaagacaa
     1021 ttcaagacga aaacacgata attttcctat agaggtagaa tcctccgtag agcaggagac
     1081 acactgtatc acaaataata agtcggcttc cggtatagta acacatgact atgatgccga
     1141 ttatatttgt ggttgtggtg aaattatgtg tcctggttgc ggtcatgacc tataacaaac
     1201 atttttgcaa actgatacct gatcaggtac aagcctgttg attattatgc tgaatatttc
     1261 atgttataaa accatcgata taaacattaa caagaaaaaa catatattgt ttatctgaac
     1321 atttatcgat ggaaaaccaa aggggaaagt atgtatctt
//
LOCUS       DAZGHF010000355         1357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGHF010000355 DAZGHF010000000
VERSION     DAZGHF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.358"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            49..951
                     /locus_tag="ACHK3A_005217"
     CDS             49..951
                     /locus_tag="ACHK3A_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO8743142.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRSNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGGDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
     gene            984..1307
                     /locus_tag="ACHK3A_005218"
     CDS             984..1307
                     /locus_tag="ACHK3A_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743143.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
BASE COUNT          429 a          211 c          339 g          378 t
ORIGIN      
        1 gaacgtacag ctgtttaagg taaaaattat tatgtgcagg agaagttgat gtccagacgt
       61 gaaaaattac tgaaacagga tctggttgct cacattcgaa aagacaaagg cagcttcaag
      121 aagctgagtg attacaccag gatcatgcac cgctttgctg aaagtctggc aaaaatgaat
      181 gtacagatca gttctgccgc gcaggtaaaa gtccggcata ttgagctata tatgagaagc
      241 cggagtaatg tttctgggcg aacaagggaa aatgaaatgt ctgcaatacg ggttatgtta
      301 cgtcaggcag gtaaatataa gatggctgat cccgaacatc ctcgtctcag caataaagcg
      361 ttaggcattt ccggctccag tcgtaaggga acaaaaaaat taatcacgga tgagcgtttt
      421 cgggagattc ttcagcgtgt ggaagcaaaa gataaagggg tggctgctgt tgttaaactg
      481 tcacgttacc tcggtctgag aaatgaagaa gctatccagt cggccaaatc attacgaacc
      541 tggaaaaaag cgttacaacg gggagatgaa aaaataagag tgatttttgg tacgaaaggg
      601 ggacgggaaa gaatgacaac ggttgttgac cgggataacg ttcttgatgc tgttaattat
      661 gcactcaaat atgccagtga acataatgga cgtcttatag acagagataa tcttaagtcg
      721 gcaatggatt attatcttaa tacattacgt cgttatggag aactgaaagg cggagatgag
      781 actgcacaca gttttcggta cagttacgct gcaagtgcca tgaaatatca tattatgaac
      841 ggttacagtt ataaggaggc acttgcactg acatcaattg atcttggtca tggtgatggc
      901 agaggacgat atattgaaca ggtttattgt cagttgttag gctatgagta ataactcatg
      961 gcgatttcgt acgggtaaat attatggatt gtactatttt gctgaaaata atctctgtaa
     1021 aagagattac aaaaaattcg cctttgtggt cacactatct ggcgcgatat gttgattccg
     1081 gatatcaggc cgagtttgaa tgtgaaggaa cagcagataa atgggatatc tatgatgaat
     1141 gggatatcta tgaatccatt gcggattatt ttgatgtggt gggagatttt aactgtgatg
     1201 tcagatggtc gggaagaaaa atactgatta aagggacctg tgtttttcct ctgggagaac
     1261 ccgttaaggg aatacataat attcctcttt ggattgatgt tgaataactt gacattcatc
     1321 ttttgtgatt taaaaatata caggcttata tgtcgct
//
LOCUS       DAZGHF010000356         1349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGHF010000356 DAZGHF010000000
VERSION     DAZGHF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.359"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1349
                     /locus_tag="ACHK3A_005219"
     CDS             <1..>1349
                     /locus_tag="ACHK3A_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO8743144.1"
                     /translation="KAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQI
                     SDSFGGPLSLLTLLKEELLGIRDASESSEDSLSDTANALAENARNAGELGRFMSVARV
                     AAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAGVISDEAGGG
                     IRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKAL
                     ASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEK
                     IWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWE
                     TTKNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDVRIRQGEMGYINRSRAT
                     GVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKH"
BASE COUNT          327 a          316 c          448 g          258 t
ORIGIN      
        1 aaagccggga tatcagtcgg acagtataag gctgccatgc gcacactgcc cgcacagttc
       61 acggatattg tcactcagct tgccggtggt cagaatccct tccttatcat gctgcagcag
      121 ggggggcaga tcagcgattc attcggtgga ccgctcagcc tgcttaccct gctgaaggag
      181 gaacttctcg ggatcaggga tgcctctgaa tcatcagagg attcgctgtc agatacggca
      241 aatgcactgg ctgaaaatgc ccggaatgcc ggtgaactgg gacgatttat gtcggtggcc
      301 cgtgtggcgg caggtggcgg ggttgccgta ctggccgcgc ttgctgctgc cgcctggcag
      361 gcagagcagg ctgaccgggc cttattgcgt tcactgaccc tgaccggagg ggcggctgcc
      421 accacaacgg cagaattgtg gaaaatggcc ggggtgatca gcgatgaagc cggtggtggt
      481 atcagacagg cggcagaaaa tctggcccgt ctggcagaaa gcgggaaata taccgccggg
      541 cagctacgga tcatggggga aacctctcag agatggctgc agacggtggg ggacgatgcc
      601 gggaaggtgg aaaaagcctt tgaagggatt gcagcagatc cggtgaaggc gctggcctcc
      661 ctgaatcagc agtataactt cctgagcgtt tcccagttac gccatattga tgagcttgag
      721 cgcacgaaag gtaaacaggc tgcggtgacg gaggcgatgt ccctgtttgc ggatgtcatg
      781 aatgcacgtc tggagcaact tgataaagcg gccacgccgg tggaaaaaat ctgggacgat
      841 gttaaaacct ggacttctga cgcatgggca tggataggtg atcatacact gggggcactc
      901 agtctgatca ctgacgtggt ggcaggaacc gttgaacagg tgaagctgct gcttgtgcag
      961 ggggatctgg cgctggctga atttattcag tcagcctggg aaacgacaaa gaatgtgccc
     1021 ggcgttggtg cgttgtttgg tgaactggca gaagagaacc gcgtatttat tgagaaaaca
     1081 aaacgcgatg aactggcgct gagaaaatcc attgcggaac gggatgtgcg tatacgccag
     1141 ggggaaatgg ggtacatcaa ccgttcgcgt gcaacaggcg tcagcaaagg tccggggcag
     1201 caggaagccg tcagccgtct ggctgaagag ctgacaggta aaaagcatac atcaccgaaa
     1261 acgcgctctg ccggggagag ggaagaggag caggcaagag aggctctgct tgcccttgaa
     1321 gctgagctca ggacgctgga aaaacacag
//
LOCUS       DAZGHF010000357         1347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGHF010000357 DAZGHF010000000
VERSION     DAZGHF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.360"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1347
                     /locus_tag="ACHK3A_005220"
     CDS             <1..>1347
                     /locus_tag="ACHK3A_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8743145.1"
                     /translation="LKNNSAYAADNFVRHGLRARLKRIARVNERFATPAFKATAARER
                     LDELLMLPQLSRDEIQRLATLTATAFSAALERTCDEVIEKTGRGDDDLFTWLLTYQPL
                     AHMALKLGITPPCWPSLEIRPDSRTAPDPEQVPGAVMRLSCATWWNSQLRHLRDIWRE
                     ELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIENEEGERFSLEDIYWAG
                     LSNPRNRRNEMMACVRGMEQVAEARGDVAFFVTVTAPSRFHSVNDDGSLNPKYCGATV
                     RDASDYLVYRFFAAVRRAINKAGLGWYGVRTVEPHHDGTPHWHMLVFTSPENEARITE
                     IMRNAAIREDRAELGDDISPRFKCEKIDPAKGTPASYIATYIGKNLDASAFMGNDPKT
                     GKPYVDKESGKTMAETVENAIGWAALHRIRQFQFFGIPPRQVWRELRRLAGQMARNP"
BASE COUNT          299 a          423 c          388 g          237 t
ORIGIN      
        1 cttaagaaca acagcgcata cgcggcggat aacttcgttc gtcacgggct gcgtgcccgc
       61 ctgaaacgta tcgcccgtgt gaatgaacgc tttgccaccc cggcctttaa ggccaccgcc
      121 gcgcgcgaac ggctggatga actgcttatg ctgccgcagc tgagccgtga cgaaatccag
      181 cgccttgcca ccctgaccgc caccgcgttt tccgccgcac tggagcgcac atgtgatgag
      241 gtcatcgaaa agaccggcag gggtgacgat gacctgttca cctggttact gacctaccag
      301 ccactggcac acatggcgtt aaaactgggt atcacgccac cctgctggcc gtcgctggaa
      361 atcaggcccg acagccgcac cgcccccgac ccggaacagg tgccgggtgc ggtcatgcgc
      421 ctttcctgtg cgacatggtg gaacagtcag ttaagacacc tccgggacat ctggcgtgaa
      481 gagctgttac gcgccgcagg cctggtgtca cgtaagacgt cggtatatat cagccatgaa
      541 gcgttactgg actggcgcga aaaacgccag cgcacccgcg attttctgaa ggcctgggac
      601 atcgagaacg aagaaggaga acggttcagc cttgaggata tctactgggc aggtctctct
      661 aatccgcgta accgccgtaa cgagatgatg gcctgcgtcc ggggaatgga gcaggtcgcc
      721 gaagcacgcg gcgacgtggc gtttttcgtc accgtcacag ccccttcccg ttttcacagc
      781 gtgaacgatg acggcagcct gaacccgaaa tactgcgggg caacggtacg tgatgccagt
      841 gattatctgg tttaccgctt tttcgccgcc gtccgcaggg ccatcaacaa ggccgggctg
      901 ggctggtacg gcgtgcgcac cgtggagccc caccacgacg gtacaccgca ctggcacatg
      961 ctggtattca ccagcccgga aaacgaagcc cgtatcactg aaatcatgcg caacgccgcc
     1021 atcagggagg accgcgcaga gctgggtgat gatatctccc cgcgttttaa atgcgaaaaa
     1081 atcgacccgg caaaaggcac accggcaagc tatatcgcga cctatatcgg taagaacctg
     1141 gacgccagcg ccttcatggg gaatgacccg aaaaccggca aaccttacgt cgataaggaa
     1201 tcaggcaaaa ccatggccga aaccgtggag aacgccatcg gctgggcggc ccttcaccgc
     1261 atccgccagt tccagttctt cggtatcccg ccccgccagg tatggcgcga actccgccgc
     1321 cttgccgggc agatggcccg taacccg
//
LOCUS       DAZGHF010000358         1346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGHF010000358 DAZGHF010000000
VERSION     DAZGHF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.361"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            134..1345
                     /gene="bluF"
                     /locus_tag="ACHK3A_005221"
     CDS             134..1345
                     /gene="bluF"
                     /locus_tag="ACHK3A_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO8743146.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELMCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
BASE COUNT          382 a          285 c          291 g          388 t
ORIGIN      
        1 ctaactcatc ttcaactttt tccatttttt atcaatgtaa agaaacagtt atgttttggt
       61 attaaaagaa agacaccgga agatatgtct gttaccgtct tactctcgcc tctcccatta
      121 ccctggacag ttaatgctta ccacccttat ttatcgtagc catatacgtg acgacgaacc
      181 tgtcaaaaaa atcgaagaaa tggtttcgat agcaaatcgc aggaacatgc agtctgacgt
      241 aacagggatc ttactgttta atggttctca ttttttccag cttctggaag gtccggaaga
      301 acaggttaaa atgatatatc gggctatatg ccaggatcca cggcactata atattgttga
      361 gctgatgtgc gattacgcgc ctgctcgccg ttttggcaaa gcgggaatgg aattatttga
      421 tttgcgcctg cacgagcgag atgacgtttt acaggccgta ttcgacaaag gcacatcaaa
      481 atttcagcta acttatgatg acagagcgct acaatttttt cgtacttttg tccttgcaac
      541 cgaacaagca acctatttcg agatccctgc cgaagactcc tggcttttta tcgctgacgg
      601 atctgataaa gaacttgatt cctgtaccct ttcaccaaat ataaacgacc actttgcctt
      661 tcatcctatt gtcgatccct tatcgcggcg gataatcgct tttgaagcca ttgtgcaaaa
      721 aaatgaagac agcccgtcag ctatagcggt tgggcagcgt aaagaagggg aaatctatac
      781 agcggatctc aaaagtaagg cgcttgcatt cgcgatggca cacgcacttg acctcggtga
      841 taaaatgatt tcaatcaatc tattacctat gaccctggtt aacgaacctg acgcagtctc
      901 ttttttactt gatgaaataa aggccaatgc tctggtgcct gaacaaatca tcgttgaatt
      961 tactgaaagt gaagtcatat ctcggtttga tgagtttgcc gaagcggtta aatcgctaaa
     1021 ggctgctggt atcagtgtag caattgatca ttttggcgca ggttttgccg gtttgttact
     1081 cctgtcacgc ttccagcctg acagaattaa aatcagtcag gaattgatta ccaatgtcca
     1141 taaaagtggg ccacggcagg caattattca ggcgatcata aaatgctgta catcacttga
     1201 aattcaagtc tctgctatgg gtgtgacaac accagaagag tggatgtggc ttgaatctgc
     1261 aggaattgag atgtttcagg gagatctgtt tgcgaaagct aaattgaatg gtatcccttc
     1321 agttgcgtgg ccagagaaaa aataat
//
LOCUS       DAZGHF010000359         1345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGHF010000359 DAZGHF010000000
VERSION     DAZGHF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.362"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..246
                     /locus_tag="ACHK3A_005222"
     CDS             <1..246
                     /locus_tag="ACHK3A_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO8743147.1"
                     /translation="HERAADVPGARLGDETQLVAQYKKAVYARAKADLMGEFATIGRR
                     ETYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            243..716
                     /locus_tag="ACHK3A_005223"
     CDS             243..716
                     /locus_tag="ACHK3A_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743148.1"
                     /translation="MNQLESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMELFVSRSRE"
     gene            713..>1345
                     /locus_tag="ACHK3A_005224"
     CDS             713..>1345
                     /locus_tag="ACHK3A_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO8743149.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRHRLLWRIARHGIIAAA
                     RRHQRNQCAPDGTPWEPRKNKKKKAGKNTGKMLKKLPRLMAVREIPGQDAVKVYFRAV
                     RYGPTASASNVAWAHHNGATIRYHGDSFRHKKPGRSVPCSLKQAQALRAWGLELKSGK
                     RWRKATLSMIRRRVSNRQAGWLLARFERDDWENFRDLHSSDGPGIRQRNSP"
BASE COUNT          331 a          382 c          415 g          217 t
ORIGIN      
        1 catgaacgcg ccgcagacgt accgggggcc aggctgggcg atgaaaccca gctggtcgca
       61 cagtacaaaa aggccgttta tgcccgtgcc aaggccgacc ttatgggcga gttcgccacc
      121 attggtcgcc gtgaaaccta tccggggcag gaaagcgagg acacacgcgc caccttactg
      181 gctgaagcct cgctggtcat gcgtaacatg ctggggcaca gacgcgcggg agtgcacatc
      241 atatgaacca gctggaaagc ctgaccgcct tcattaccga aaatctgccg cctgacgcca
      301 tgcagttctt catgagcagt caggaagacg cgcagcttat ccgcagtcag aaggcgctgg
      361 gtaacgacca gatccgcatg ggcgtgatga cgtacaccgg cgtgattgca tgggataact
      421 tcccgttccg caaatactca ccggggctgg tttacgcgct ggtcatggcc tggctggaag
      481 agcacgcgga cccgctgagg gaggaattcc gcccggagga tgtgaacgtc gacccgcagt
      541 ttaacgatga ggattcgtgc cttctcgata tcacggtggg actggctgac ccgctgatca
      601 tccgggtgca ggaagacggc cccgttccgt acagggggaa acgctggaca ctggttaacc
      661 ctgaaatctg gacggcggaa accatggagc tgtttgtcag caggagccgc gaatgataag
      721 cggccagctg aacccgcagc agctggcaga agcgcaggcg atgcttcagt cgctggatct
      781 gcccccgaaa aaacgccatc gcctgttatg gcgtattgcc cggcacggca ttatcgccgc
      841 agccagacgc catcagcgca accagtgcgc accagacggc acgccgtggg aaccacggaa
      901 aaataaaaaa aagaaggcag gcaagaacac cggaaagatg cttaaaaaac tcccgcgcct
      961 tatggcagtg cgggagatac cgggacagga cgcggtgaag gtgtatttcc gcgcagtcag
     1021 atacggcccg acagcgtcgg cgagtaacgt cgcgtgggca catcacaacg gcgccaccat
     1081 ccgctatcac ggcgacagct tccggcacaa aaaaccgggg cgttccgtac cgtgttccct
     1141 gaagcaggca caggcgctga gagcatgggg gcttgagctg aaaagcggca agcgctggcg
     1201 aaaagccacc ctgtcaatga tacggagaag ggtatctaac cgacaggcag gctggctgct
     1261 ggcaagattt gagcgcgacg actgggaaaa ttttcgcgac cttcacagca gtgatggtcc
     1321 gggaatacga caacgtaaca gccca
//
LOCUS       DAZGHF010000360         1339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGHF010000360 DAZGHF010000000
VERSION     DAZGHF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.363"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1130
                     /locus_tag="ACHK3A_005225"
     CDS             <1..1130
                     /locus_tag="ACHK3A_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HFO8743150.1"
                     /translation="TLKNFKAYKEQSFDFSNLTVFCGNNSVGKSTAIQAIGMFLQSDM
                     NGNSNLKINGDLVHVGDFDDIHNFSMREDDVLYLKYTFEDDTACWGYGIEEGDAREEL
                     SRKNELPFIGCDKQLRTITNSLKDNFSFQYLEAERFGPRDNIPLSQHNYHEGWLGKNG
                     EHTIEVLESLVTRRRLNFSAQEDPRKHEGIINNNVFNHIEAWMSEISPGHRISPRIEA
                     RANVAFNVIIPSPGQLTKPINIGFGFSYALSIVTALMLASKNELVIIENPEAHLHPRG
                     QSYLGRLIALSAKAGIQVIIETHSDHLLNGIRVIARTDPTFDASLFTLYYISRENDET
                     SVAKIKINNEGKLSEWPRGFFDQQSQDMYTIMTGSFDHPNI"
     gene            1150..>1339
                     /locus_tag="ACHK3A_005226"
     CDS             1150..>1339
                     /locus_tag="ACHK3A_005226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743151.1"
                     /translation="MKAGVFLIPECFDFNTDILSLSSDLDLASKLVVNTRSEFIHFMK
                     SREFDSKIAELLFSTSPDY"
BASE COUNT          438 a          203 c          259 g          439 t
ORIGIN      
        1 taacattaaa aaatttcaaa gcttataaag aacagagttt tgattttagt aatttaactg
       61 tattttgcgg taataattct gttggtaaaa gtacggctat tcaagctatc ggtatgtttc
      121 tccaatcaga tatgaatggt aacagtaatc taaaaataaa tggcgattta gttcatgtcg
      181 gagacttcga cgatattcat aatttcagta tgcgtgagga tgatgtttta tatttaaaat
      241 atacatttga agacgatacg gcttgctggg gatatggtat agaagaaggg gatgctagag
      301 aagaactttc taggaaaaat gaattacctt ttattgggtg tgataaacaa ttaagaacta
      361 taacaaactc acttaaagat aacttttcat tccaatattt ggaagcggag agatttggtc
      421 caagagataa tattccatta tcacagcaca attatcatga aggttggctt gggaaaaatg
      481 gggaacatac aattgaagtt cttgagagtt tggtcacaag gaggcgttta aatttttctg
      541 ctcaagagga tcctaggaaa catgaaggaa ttattaataa taatgttttc aatcatattg
      601 aagcatggat gtctgagatt tcaccagggc atcgtatctc acctagaatt gaggctaggg
      661 cgaatgttgc atttaatgtc ataatccctt cacctggaca attaacgaaa cctattaaca
      721 taggatttgg atttagttat gctcttagta ttgtcactgc tttaatgctg gcatcaaaaa
      781 atgaactcgt tattattgaa aatcctgagg ctcatctaca tccaagaggt caaagctatt
      841 taggacgact tattgcttta tcagcaaaag cagggatcca ggttattatt gagactcata
      901 gtgatcatct tcttaatggc ataagagtaa ttgcacgcac agatccaact tttgatgctt
      961 ctctttttac tctttactat atttcacgag aaaatgatga aacatctgtc gccaaaataa
     1021 aaataaataa tgaagggaaa ctttccgaat ggcctagagg gttctttgat caacaatcac
     1081 aggatatgta tacgataatg acaggctctt ttgatcatcc caatatttaa tattgtaggg
     1141 acatataaaa tgaaagctgg cgtgtttctt attcctgaat gttttgattt caatacggat
     1201 attttatctt taagttcgga ccttgatctg gcttcgaaac tcgtagtaaa tacccgttca
     1261 gaatttattc actttatgaa atctagggag tttgactcga agattgcgga gttattgttt
     1321 tccacatcac ctgattatg
//
LOCUS       DAZGHF010000361         1335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGHF010000361 DAZGHF010000000
VERSION     DAZGHF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.364"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..778
                     /gene="ompT"
                     /locus_tag="ACHK3A_005227"
     CDS             <1..778
                     /gene="ompT"
                     /locus_tag="ACHK3A_005227"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO8743152.1"
                     /translation="SQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVD
                     QDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWFLNEPNYRLGLMAGYQESRYSF
                     TARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFK
                     YSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRVT
                     NKKGDTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1010..>1335
                     /locus_tag="ACHK3A_005228"
     CDS             1010..>1335
                     /locus_tag="ACHK3A_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO8743153.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVF"
BASE COUNT          422 a          273 c          280 g          360 t
ORIGIN      
        1 cagtcaactt gactggaaat tcaataacgc tgcaattatt aaaggtgcaa ttaattggga
       61 tttgatgccc cagatatcta tcggggctgc tggctggaca acactcggca gccgaggtgg
      121 caatatggtc gatcaggact ggatggattc cagtaacccc ggaacctgga cggatgaaag
      181 tagacaccct gatacacaac tcaattatgc caacgaattt gatctgaata tcaaaggctg
      241 gttcctcaac gaacccaatt accgcctggg actcatggcc ggatatcagg aaagccgtta
      301 tagctttaca gccagaggtg gttcctatat ctacagttct gaggagggat tcagagatga
      361 tatcggctcc ttcccgaatg gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc
      421 ctacattggc ttgactggaa gttatcgtta tgaagatttt gagctaggtg gcacatttaa
      481 atacagcggc tgggtggaag catctgataa cgatgaacac tatgacccag gaaaaagaat
      541 cacttatcgc agtaaagtca aagaccaaaa ttactattct gttgcagtca atgcaggtta
      601 ttacgtaacg cctaatgcaa aagtttatat tgaaggcgca tggaatcggg ttacgaataa
      661 aaaaggtgat acttcacttt atgatcacaa tgataacact tctgactaca gcaaaaatgg
      721 tgcaggcata gaaaactata acttcatcac tactgctggt cttaagtaca ccttttaaca
      781 acgttaaata gattttctcc ccggtggtat tgtatagccc cggggaattt gtttaccgtt
      841 aaaagcaaac aatatcccct aatcttgtaa gcatttaacc tgcgtcctgg cgtgtaacct
      901 cttaccgcaa cacaagactc aatatattca aattcgttat gccgtggcat catttaaccc
      961 cttgtaattc atcgctataa ttgatttaat tcacaaataa aactataaca tggtgaaact
     1021 aatgaaaaaa aacacagatg atggggctaa aatttacaca ccacttaccc taaagcttta
     1081 tgactggtgg gttttgggtg tatcaaatcg gcttgcatgg ggatgtccta caaaggaaca
     1141 ccttcttcca cactttctgg aacatttagg taacaaccat ctggatattg gcgttggaac
     1201 tgggttttac cttactcacg tacctgagag tagtctgata tctttaatgg atttgaacga
     1261 agctagcctg aacgcggcat cgacaagggc tggggaatca aaaattaaac ataaaattag
     1321 ccatgatgtt tttga
//
LOCUS       DAZGHF010000362         1326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGHF010000362 DAZGHF010000000
VERSION     DAZGHF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.365"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            65..1084
                     /locus_tag="ACHK3A_005229"
     CDS             65..1084
                     /locus_tag="ACHK3A_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO8743154.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDENTGEVVDKQLRGRDFLEYFSNR
                     NPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRRQLVKFRTMQTNSLHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLITVLENQYNRLNELDLLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYVRNHV
                     SIRPY"
BASE COUNT          379 a          307 c          368 g          272 t
ORIGIN      
        1 atttccggca tcatagtgcc caatacggga tgcagaaagc tccctcagac tggagactcc
       61 acaaatgaaa tatacaccgg ttggcgttga tatcgcaaaa catctgattc aggttcactt
      121 cattgatgag aacacaggtg aagtggtgga taagcaactt cgcggcagag attttctgga
      181 atatttcagc aaccggaacc cctgcctgat tggtatggag gcctgtggag gctctcagca
      241 ctgggcacgg gagctgacaa agctggggca taaagtccgc ttgttacagg cccgctttgt
      301 taaggcattc gtcatgggaa acaagaacga cgtgatggat gcccgggcta tctggatggc
      361 ggttcagcag cctggtaaag aaattgccgt aaaaacagaa gaacagcagt cagttcttgt
      421 gttgcaccgg acccgcaggc aactggtgaa gttccgcacc atgcagacta actcattgca
      481 cggcacgctc ctggaatttg gtgaaaccat tcacaaaggt cgggcagcga tggagcgaga
      541 gtttcctgaa gcactggagc ggatgaaaga gagactgcca ccgtatctca ttactgtgct
      601 ggaaaaccag tacaaccggc tgaatgagct ggacttactg atagaggata ttgaaaaaca
      661 gcttaccagc gtggcgaggc agaatgaaac ctgtaagcgg ttgctggata ttcccggcgt
      721 tggaccactt attgcgacgg cagcggtggc caccatgggg gaagcatcag cgtttaaatc
      781 agggcgagag ttcgccgcat atgtcggtct ggttccaaaa caaaccggct ccggaggtaa
      841 agtacgcctg ctggggataa gcaaacgtgg tgacacttat ctcaggacat tatttatcca
      901 cggagcaaga gcggtggcat tagtagctaa agaaccaggc ccgtggataa ccgaactgaa
      961 aaaacgtcgt ccggccagtg tggcaatcgt cgccatggca aacaagctgg cacgaacagt
     1021 atgggcgata acggcccatg accgtaagta tgtcaggaac cacgtcagta tcagaccata
     1081 ttaatcgctg ataccgttaa acaatgaact cttaacaaaa gggtgaatgc tgaaaggttg
     1141 ctatggcggc ccgagtgatg acaacgacag gtaagaccgt gacctgataa acctgaataa
     1201 gctcctggac ataaagtccg taatgggaat aaggctcagg tcagcgaatc tcatagtggc
     1261 ccgcagctct gactgcaata aggccggata cagagctgca actactcgtc gatgtcagaa
     1321 caacgg
//
LOCUS       DAZGHF010000363         1326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGHF010000363 DAZGHF010000000
VERSION     DAZGHF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.366"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT          419 a          302 c          274 g          331 t
ORIGIN      
        1 tttctccctg acgacacggt taaacgttgc gatccttcgg gtagtgactg cctgtgcgcc
       61 agggtagcgc agattgttta ggtattgagg ggtaacaaat cggtgttgca caccgactgc
      121 agcatttgct gtacctgtct ccaggtcgcc cccgacacca cctctaataa gtggtatcgc
      181 cgattcgaca tctcactaca taaaactcct taaaaaacaa ttgattctgc aaaaaaccat
      241 ttatggacgt tattcacaaa atcgtccgta aatttgcaga agaggttgaa aaaacactct
      301 cacgccgggt cacttcttca aatgttaaaa aacatcacgt gaaacggcag ttcgtgcggg
      361 ttcgttaagc agacgcgcgt gcgtcacttt gtcatcgtga cacgctgacg aaagtgacgc
      421 cgcgcagctt cagacgccag atacacgcgt aagcgaccaa atttcaaatg gtcttataca
      481 gcgcgcacag tcgcaggaag tgctggcaaa caaatgaaaa gaataaaaga agtcaatacc
      541 gccattacgc agtaagaaca ttccggcatc accggatgga taaggatcac acaaccactg
      601 ctgtgtattg cccgtaataa agggcgcggt taaaagaaac cttcatcgtc atgactgacg
      661 atatctgctc tcaaaggtgg gcagatttac caccaccaga aacatcctgg ctgcacatcc
      721 cctttcaggt tacgggggtg ttgatcaccc gaaggcgttc ctctcagatc aacggaacat
      781 tggtgcccac cgaagcggga tgtggtcacc cgaaggcgct cctctcagac cacttatgga
      841 gcattggtgg tcgccgaagc gacagataat gcattctgag catatcagag cgcataaaag
      901 gcgtcaatta tccatgctcc ctcacgaaac agaagctgaa ctcttaacac ctgctatcgg
      961 aactcactga ataaacaact aaaaaggaca ttgtgaaaat taacatcaac agtatgtaaa
     1021 gtgatttgtt ttgtcttaaa tgggtgaatg taaaaaaata ttctatacaa tccacacatc
     1081 cttgtatcat atatcaagta aacatccaca gagataacag cttatttcaa ataagcagat
     1141 tctgtttgat acttcagaaa aatagtatat gctcctgatt tactgatgtt ataagttata
     1201 caggatagtt aaatgtgtga ttttataaaa tcatgttatg cagcaccaag aattaacaaa
     1261 atggctgatt ttttctcatc aacagcatct ggagtaaaac ataaaccttt gaagcatgta
     1321 aaaatt
//
LOCUS       DAZGHF010000364         1311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGHF010000364 DAZGHF010000000
VERSION     DAZGHF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.367"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1253)
                     /locus_tag="ACHK3A_005230"
     CDS             complement(<1..1253)
                     /locus_tag="ACHK3A_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO8743155.1"
                     /translation="MLMKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSL
                     AAVTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTG
                     GQWINAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQW
                     VHGGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTT
                     INKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRD
                     GWQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSSAATVTGTNRLGAF
                     SVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLA
                     DSGAAVSGTRSDGTAFRIGGGQADAL"
BASE COUNT          245 a          422 c          329 g          315 t
ORIGIN      
        1 agggcatccg cctgaccgcc tccgatacgg aatgccgttc cgtcgctccg ggtaccactg
       61 acagcggcac cggaatcggc cagcagtaca ccgccattcc ccatggatac ggtggtggcg
      121 gcaccaccgt tgcggacatc cagcgttccg ccatcatcca cacgggtatt agtggctgtg
      181 tgtccgctca gcacatccag acgtccgcca ttttccagta cgacattatc agccttaccc
      241 gccacaacgg agaatgctcc caggcggttt gtaccggtga cggttgcagc tgaactggta
      301 accagtgcac cgcccgtgtt ctgggtgaca tcagacgctt taccgccggc attcacctgc
      361 agctttcctt tctggttgat ggtggtatgc gcggcagttc ctccttcctt aatcacctgc
      421 cagccatcac ggtttatcag cgtctctgtt gccgtgccac cgttgtgtac atactggtta
      481 cccccctcca gccgggtgtt atgtgcctct ccgtgtaccg tctggtcacc accggcgtaa
      541 atgagggtgt cccgtgccat ccccgaagac cagataacct gccggccatt tttgttgatg
      601 gtggtggatt cagccgtccc tccgaccatc tgaccgctgg acacattttc agactccgga
      661 ccaccttctg caccggtgtt gacgatggtt ccggttgcca gtccgccatg ttttatggtc
      721 tggtaaccat cctggttaat aattgtaccg gcagctttcc ctcccccgtg tacccactgc
      781 tcacctctgt tatccagcgt ggtgttcacc gccagtccgt taaggctctg ccctccgcca
      841 tcacgaataa ccgtatcact ggcagttcct cctgcctgca caatctgacg accatttgcg
      901 gtgacagtgg tgtttctgcc tgtgccaccc gcgtttatcc attgcccgcc ggtgttttcg
      961 tcactgtccg gccccagctc aagcccggta ctgacagtca cgccatcagc tgttccggat
     1021 acaaactggt tgtcatggtt taccagtgtt ccgccattca ctgtttcacc cgggtgcaca
     1081 acgatgtcag cagccagcac cgggagtgac gtgaccgcgg caagagacag tgcaaccgcc
     1141 acaccgccac gtttaccccg tgcgcgggcc agttcggagg caaccacgaa agcgcccgta
     1201 atgtgattcc ataccagcct gtagcaggta ttcagatgtc gtttcatcag catttcctta
     1261 atcagagtga gggtgaataa aaaagccggt atccacagat gtggataccg g
//
LOCUS       DAZGHF010000365         1293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGHF010000365 DAZGHF010000000
VERSION     DAZGHF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.368"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..373)
                     /locus_tag="ACHK3A_005231"
     CDS             complement(<1..373)
                     /locus_tag="ACHK3A_005231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743156.1"
                     /translation="MTTCSELKERARFDGMHDASLEVAWQLLMTKSVSTITTEIISGA
                     DMSNFSVIIRERYKHAIAKSLLNHLSPDVVSDIIGLSHDEFNQQYKGNLTRQQHLRDI
                     AVSLTDKGFTESSACNIVNLDL"
     gene            complement(457..>1293)
                     /locus_tag="ACHK3A_005232"
     CDS             complement(457..>1293)
                     /locus_tag="ACHK3A_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO8743157.1"
                     /translation="SIFKSFMIEETDRFTKSGVHTRIDRIYIHNDTAMSKSIVSDDDN
                     FAKLNTMSYREFLDNLSQTIFAKHDTLHKVFCDIKDTINITDYLNIQTIRKIKSGFSK
                     YLLNNSFNKFSLGYNVISGSIHPTKFTNADGNHLHEVLSGDLGVLQDNTKAPLDSYLF
                     EEVFYDSELERLNITDGEIHSVAVFTKIPKNSIKIPVAGGYTYSPDFAYVVKTTKGDY
                     LNFIIETKNVEGKESLRKEELDKIKHAQKLFEQVSKSVKVTFITQFSGDRIHDLIKNV
                     IQ"
BASE COUNT          385 a          219 c          229 g          460 t
ORIGIN      
        1 ttaggtccag gttgacaata ttacatgcag atgattctgt gaatccttta tctgttaaac
       61 ttacagcaat atctctcagg tgttgttgtc tggttaaatt acctttatat tgttgattga
      121 actcatcatg agataaaccg atgatgtcag aaacaacatc gggagataaa tgattgagaa
      181 gagattttgc tattgcatgt ttatatcgtt ccctgataat cactgaaaaa ttggacatgt
      241 ctgcaccgga tattatctca gtagtgatag tggatacact tttcgtcata agtaattgcc
      301 atgccacttc aagagaagca tcatgcatac catcaaaacg ggctctttcc ttcagttcag
      361 aacatgttgt cattgaactc acctgcgaat tttgtgatgt gtgtataatt ataaagtggg
      421 ggtgaaacca cctccacttt gttatcatgc atgtgtttac tgaatcacat tttttatcag
      481 atcatggatt ctgtcacctg agaattgagt tatgaaagtt acttttacag atttacttac
      541 ctgttcgaaa agtttttgtg catgctttat tttatccagt tcttctttac gtaaactttc
      601 tttaccttcg acattttttg tttcaataat aaagttaagg tagtcaccct ttgttgtttt
      661 tacaacataa gcaaagtcag gagaatatgt gtatccacct gccaccggaa ttttaattga
      721 gtttttcggt atctttgtaa aaacagcaac agagtggatc tctccgtctg ttatattgag
      781 tctttcaagc tctgaatcat aaaaaacttc ttcaaaaaga taagagtcca ggggggcttt
      841 tgtattatcc tgtaatactc caagatcacc tgataatacc tcatgcaggt gattaccgtc
      901 tgcattagtg aattttgtag gatgaattga gcctgaaatc acattatatc caaggctgaa
      961 tttgttaaat gaattgttca acaaatactt gctaaaccca gatttaatct ttctgattgt
     1021 ctgaatgttc aggtagtcgg tgatattgat agtgtctttt atatcacaaa aaaccttatg
     1081 taaggtatcg tgtttagcaa aaatagtttg tgataagtta tcaagaaatt cccgatagct
     1141 cattgtgttt agtttagcaa agttatcatc gtcactgacg atacttttcg acatcgccgt
     1201 atcattatga atataaattc tatcaatgcg agtatgaaca cctgatttag taaatctatc
     1261 tgtttcttca atcatgaatg atttgaaaat gga
//
LOCUS       DAZGHF010000366         1291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGHF010000366 DAZGHF010000000
VERSION     DAZGHF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.369"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..246
                     /locus_tag="ACHK3A_005233"
     CDS             <1..246
                     /locus_tag="ACHK3A_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO8743158.1"
                     /translation="HERAADVPGARMGDETQLVAQYKKAVYARAKADLMGEFATIGRR
                     ETYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            243..716
                     /locus_tag="ACHK3A_005234"
     CDS             243..716
                     /locus_tag="ACHK3A_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743159.1"
                     /translation="MNQLESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMELFVSRSRE"
     gene            713..>1291
                     /locus_tag="ACHK3A_005235"
     CDS             713..>1291
                     /locus_tag="ACHK3A_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743160.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQTAPDGTAWAPRKRGKGKMFRKLPRLLAVREMPGQDAVKVYFRNGNHTSGRS
                     RISAGAVGWVHHNGAEIRVSADSFSGQDQNGQTATRRQAKRLRELGFKVRQKGRWRKA
                     SMKYLLTLSRAQAGLLITIMEDQIIAGPRQQKA"
BASE COUNT          300 a          371 c          405 g          215 t
ORIGIN      
        1 catgaacgcg ccgcagacgt accgggggcc aggatgggcg atgaaaccca gctggtcgca
       61 cagtacaaaa aggccgttta tgcccgtgcc aaggccgatc ttatgggcga gttcgccacc
      121 attggtcgcc gtgaaaccta tccggggcag gaaagcgagg acacacgcgc caccttactg
      181 gctgaagcct cgctggtcat gcgtaacatg ctggggcaca gacgcgcagg agtgcacatc
      241 atatgaacca gctggaaagc ctgaccgcct tcattaccga aaatctgccg cctgacgcca
      301 tgcagttctt catgagcagt caggaagacg cgcagcttat ccgcagtcag aaggcgctgg
      361 gtaacgacca gatccgcatg ggcgtgatga cgtacaccgg cgtgattgca tgggataact
      421 tcccgttccg taaatactca ccggggctgg tttacgcgct ggtcatggcc tggctggaag
      481 agcacgcgga cccactgagg gaggaattcc gcccggagga tgtgaacgtc gacccgcagt
      541 ttaacgatga ggactcgtgc cttctcgata tcacggtggg actggctgac ccgctgatca
      601 tccgggtgca ggaagacggc cccgttccgt acagggggaa acgctggaca ctggttaacc
      661 ctgaaatctg gacggcggaa accatggagc tgtttgtcag caggagccgc gaatgataag
      721 cggccagctg aacccgcagc agctggcaga agcgcaggcg atgcttcagt cgctggatct
      781 gccaccaaaa aaacgccgtc gcctgttatg gcgtattgcc cggcacggca ttatcgccgc
      841 agccagacgc catcagcgcg atcagaccgc gccggacggc acagcatggg caccccggaa
      901 acgcggcaag gggaaaatgt tcagaaagct gccgcgtctt ctggcggtcc gtgagatgcc
      961 cggacaggat gccgtaaagg tttatttccg taacggcaat cacaccagtg gcaggagccg
     1021 catcagtgcc ggtgccgtgg gctgggttca ccacaacggc gcagaaatcc gggtcagtgc
     1081 tgacagcttc agcggtcagg accagaacgg gcagaccgcc acccgccgac aggcaaaacg
     1141 actgcgggag ctgggcttta aggtacgcca gaaggggcgc tggcggaaag catccatgaa
     1201 atacctgtta acgctttcgc gggcgcaggc cgggttgctg atcaccatta tggaagatca
     1261 gattatcgcc ggaccacgcc agcagaaggc c
//
LOCUS       DAZGHF010000367         1287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGHF010000367 DAZGHF010000000
VERSION     DAZGHF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.370"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..260)
                     /locus_tag="ACHK3A_005236"
     CDS             complement(<1..260)
                     /locus_tag="ACHK3A_005236"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743161.1"
                     /translation="MAFHDEAEHTQHDVRDRTDCAQRQHGQRDVTQPAGDVREHVQPA
                     HERRADIGKTASQWCENGVEGVTDGGPDVVEGGHKAVPRVGHG"
     gene            249..1220
                     /locus_tag="ACHK3A_005237"
     CDS             249..1220
                     /locus_tag="ACHK3A_005237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743162.1"
                     /translation="MEGHKAIIKGARAAWSLYTLALTKVRSAIIATSLAARLAGVSFL
                     TMSAPVLLVAAAIAALVVVAIKFWQPIKAFIRGFIDGFTSALDAMSPVTAAFGDIARA
                     LAPVWLGIKTLFSAFSDLFAPVQLTTGQMNSVTRAGETCGRVVAAAVSMISPALEVVI
                     SVIRTLIDIFAIVIRGWQDVVAAFDPTAPVESFRKMAGIIGNVFGDLWNVIKNSFNRT
                     WNWLAEKLNKIPGISIDMKPTEQVNTMTRGATGSTVPPQIAETAVPALAGNRISAELP
                     PGGIRNQIRTDTSTRIDNSRTFGNLTINTQNPMTPGQLDEWTELYAG"
BASE COUNT          270 a          395 c          370 g          252 t
ORIGIN      
        1 ccatgaccga cccgtgggac cgccttgtgg cctccttcta caacatccgg gccgccatcg
       61 gtaacgccct ctaccccatt ctcgcaccac tggctggccg ttttgccgat atcggcgcga
      121 cgttcgtgcg ctggctgaac atgttcccga acatcgcccg ctggctgggt tacatcacgc
      181 tgaccatgct ggcgctgggc gcagtcggtg cggtcacgaa catcgtgctg ggtgtgttca
      241 gcttcatcat ggaaggccat aaggccatca taaagggcgc cagggcagcc tggagtctgt
      301 acacgcttgc actcacaaaa gtacgcagcg ccatcattgc caccagtctt gccgcgcgtc
      361 tggcgggcgt gtcgtttctg accatgagcg ccccggtcct cctggttgcc gccgccatcg
      421 ctgctctggt ggtggtggcc attaagttct ggcagcccat caaagccttt attcgcggct
      481 ttattgatgg ttttaccagc gcactggacg ccatgagccc ggtgactgcc gcgttcggtg
      541 acattgcccg ggcgctggcc ccggtatggc tgggcataaa aacactgttc agtgccttct
      601 ccgatctgtt tgcaccggta cagctgacca cgggacagat gaacagcgtc acccgtgccg
      661 gggaaacatg cggtcgcgtg gtggccgcag cggtaagcat gatatccccc gcgctggagg
      721 tggttatcag cgtgatcaga accctgatcg acatcttcgc catcgtcatc agagggtggc
      781 aggacgtggt ggctgcattt gacccgaccg ccccggtcga atccttccgg aaaatggcgg
      841 ggatcatcgg taacgtcttc ggggacttat ggaacgtcat taaaaactcg tttaaccgga
      901 cgtggaactg gcttgcggaa aagctgaaca aaatccccgg tatcagtatc gacatgaagc
      961 ccacggagca ggtgaacacc atgacgcggg gcgcaacagg cagcaccgta cctccgcaga
     1021 ttgcagaaac cgccgtcccg gcactggcag gcaaccgcat cagcgcagag ctgccacccg
     1081 gaggcatacg caaccagata cgcacggaca cctcaacccg tatcgacaac agccggacgt
     1141 tcggcaatct gacaatcaac acgcagaacc cgatgacgcc ggggcaactg gatgaatgga
     1201 cggagcttta cgcgggatga acacgccatc gctttacatc gacatgctta tcactgacgg
     1261 cagttttacg ctgaacagcg gcaacga
//
LOCUS       DAZGHF010000368         1287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGHF010000368 DAZGHF010000000
VERSION     DAZGHF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.371"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1220
                     /locus_tag="ACHK3A_005238"
     CDS             <1..1220
                     /locus_tag="ACHK3A_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8743163.1"
                     /translation="MTDPWDRLVASFYNIRAAIGNALYPILAPLAGRFADIGATFVRW
                     LNMFPNIARWLGYITLTMLALGAAGAVTNIVLGVFSFIMEGHKAIIKGARAAWSLYTL
                     ALTKVRSVIIATSLAARLAGVSFLTMGAPVLLVVAAIAALVVVAIKFWQPVKAFIRGF
                     IDGFTGALDAMSPVTAAFGDIARALAPVWQGIKTLFGAFSDLFAPVQLTTGQMNSVTR
                     AGETCGRVVAAAVSLISPALGVVISVIRNLIDIFTIVIRGWQDVVAAFDPTAPVESFR
                     KMAGIIGNVFGDLWNVIKNSFNRTWNWLAEKLNKIPGISIDMKPTEQVNTMTRGATGS
                     TVPPQIAETTVPALAGNRISAELPPGGIRNQIRTDTSTRIDNSRTFGNLTINTQNPMT
                     PGQLDEWTELYAG"
BASE COUNT          266 a          396 c          374 g          251 t
ORIGIN      
        1 ccatgactga cccgtgggac cgccttgtgg cctccttcta caacatccgg gccgccatcg
       61 gtaacgccct ctaccccatt ctcgcaccac tggctggccg ttttgccgat atcggcgcga
      121 cgttcgtgcg ctggctgaac atgttcccga acatcgcccg ctggctgggt tacatcacgc
      181 tgaccatgct ggcgctgggc gcagccggtg cggtcacgaa catcgtgctg ggtgtgttca
      241 gcttcatcat ggaaggccat aaggccatca taaagggcgc cagggcagcc tggagtctgt
      301 acacgctcgc actcacaaaa gtacgcagcg tcatcattgc caccagtctt gccgcgcgtc
      361 tggcgggcgt gtcgtttctg accatgggcg ccccggtcct cctggttgtt gccgccatcg
      421 ccgctctggt ggtggtggcc attaagttct ggcagcccgt caaagccttt attcgcggct
      481 ttattgatgg ttttaccggc gcactggacg ccatgagccc ggtgactgcc gcgttcggcg
      541 acattgcccg ggcgctggcc ccggtatggc agggcataaa aaccctgttc ggtgccttct
      601 ccgatctgtt tgcaccggta cagctgacca cgggacagat gaacagcgtc acccgtgccg
      661 gggaaacatg cggtcgcgtg gtggccgcag cggtaagcct gatatccccc gcgctggggg
      721 tggttatcag cgtgatcaga aacctgatcg acatcttcac catcgtcatc agagggtggc
      781 aggacgtggt ggctgcattt gacccgaccg ccccggtcga atccttccgg aaaatggcgg
      841 ggatcatcgg taacgtcttc ggggacttat ggaacgtcat taaaaactcg tttaaccgga
      901 cgtggaactg gcttgcggaa aagctgaaca aaatccccgg tatcagtatc gacatgaagc
      961 ccacggagca ggtgaacacc atgacgcggg gcgcaacggg cagcaccgta cctccgcaga
     1021 ttgcagaaac caccgtcccg gcactggcag gcaaccgcat cagcgcagag ctgccacccg
     1081 gaggcatacg caaccagata cgcacggaca cctcaacccg tatcgacaac agccggacgt
     1141 tcggcaatct gacaatcaac acgcagaacc cgatgacgcc ggggcaactg gatgaatgga
     1201 cggagcttta cgcgggatga acacgccatc gctttacatc gacctgctta tcactgacgg
     1261 cagttttacg ctgaacagcg gaaacga
//
LOCUS       DAZGHF010000369         1263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGHF010000369 DAZGHF010000000
VERSION     DAZGHF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.372"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            183..560
                     /locus_tag="ACHK3A_005239"
     CDS             183..560
                     /locus_tag="ACHK3A_005239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743164.1"
                     /translation="MIKTLFDPETMSREDMLREIQLLRDSRDLALELVFGYPETKAAE
                     QIELNDDEITLLKVKLPEHHFQWLTGDGDEALTVGRLGDFADLADDFSVLAGEIDPDL
                     EEPQEEVISRGPCGVVFCLIEPD"
     gene            829..1251
                     /locus_tag="ACHK3A_005240"
     CDS             829..1251
                     /locus_tag="ACHK3A_005240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743165.1"
                     /translation="MEDWRTKPRTGASQFAGYEEEIRLLKEKKYTNKQIYDAISQKGI
                     TIGQKMFYIYMRRMFNDELYIKPSASKNKKEKTVLSDNIIKETTAWNNVNIRSLRLIK
                     DLESYGLTPEIVREWREPNEMAVRQHLTVLISKKGIIK"
BASE COUNT          448 a          203 c          294 g          318 t
ORIGIN      
        1 gacagtcctg taaaagcaat aactcaattt acaaaggtaa acatatggta ttgggatgaa
       61 atgttaaagc aacttatgaa taaattatga cagacaaggc ggggaaaccc gccttactct
      121 gtattattac ttgtgttagc atagttaaaa acaggcatta ataaattaaa cgagatatat
      181 ttatgattaa aaccttgttc gatccagaaa ccatgagcag ggaggatatg cttagggaaa
      241 ttcagctact gcgcgactca cgtgatctgg cgcttgaact ggtgttcggc tatccagaaa
      301 caaaggccgc agaacaaatt gaattgaatg atgatgaaat aacccttctg aaagtgaaac
      361 taccggaaca ccattttcaa tggttaacag gagatggaga tgaggcgcta acagttgggc
      421 gattaggtga ttttgcagat ctggctgacg attttagcgt tctggccgga gagatagatc
      481 cagacctgga agaaccacag gaagaagtta tatcccgtgg gccttgtggt gtcgtatttt
      541 gtttgattga gcctgactga tcttgtgtgt gaggtgcagt aaatgccgcg tctgcggcat
      601 gggaggcgac aaaaacaagt taaatataat tggctgtaaa gtgtcgccgt gacgcggcta
      661 cgccgcccgg atgtaaataa atcacaggaa cacctcctcc ccctgccaga cgtgtttgtg
      721 tctggcgcag aatgcgccag acaaccatat atcaggtaac acgttcctta atggtcgaag
      781 acaagcaatt gtaacagtaa caggcttgaa ctttatggag atgatgaaat ggaagattgg
      841 cgtactaagc caagaacagg agcgtctcaa tttgctgggt atgaagagga aataagatta
      901 ttaaaggaaa agaaatatac gaacaaacaa atatatgacg caatatccca gaagggaata
      961 acaataggac aaaaaatgtt ttatatatat atgagacgca tgtttaatga cgaattatat
     1021 ataaaaccaa gtgcatcaaa aaataagaaa gaaaaaactg ttttgagtga taatataata
     1081 aaggaaacta cagcgtggaa taatgttaat ataaggagtt tacgattaat aaaagatctt
     1141 gagtcttacg gtcttacccc tgaaattgtt cgagagtgga gagaaccaaa tgagatggct
     1201 gtaagacaac acttgacagt attaattagt aagaaaggaa taataaaatg attctgaaaa
     1261 ttg
//
LOCUS       DAZGHF010000370         1257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGHF010000370 DAZGHF010000000
VERSION     DAZGHF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.373"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..982
                     /locus_tag="ACHK3A_005241"
     CDS             <1..982
                     /locus_tag="ACHK3A_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743166.1"
                     /translation="DDTRTSHALVKHNYQRLLDKIDSGSDRYSPMEYWRALHNPDYRD
                     VLQKTIVKHPSDWYFKKDDAIWQPFLNALKKDAPEWKKYSEDFLDKMAWMQDVTTEKL
                     GPLLWHMHPIMFLGCMKKSSGIWPLGKTSERYESGGRGPGVISSGKGDHGGVSYGMYQ
                     LSSKMGTVNKYIIMSKYKDLFEGKKPATPEFNEIWKKIALIHKEEFAQDQHLFIKKTH
                     YQVQLDYLASKGLNFSHSLAAINDMIWSTSVQYGPTTGLISRALKGKNLETLTDADVI
                     TIVQDYKINHVEDLFPSSPTWWDDLKTRALTEKKDLLELNELNLELKNED"
     gene            972..>1257
                     /locus_tag="ACHK3A_005242"
     CDS             972..>1257
                     /locus_tag="ACHK3A_005242"
                     /inference="COORDINATES: protein motif:HMM:NF045244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743167.1"
                     /translation="MKIKLFALLIFLPIISFAKEPHVCNSSMQSHPDIIDCIQISTRK
                     IEDSIKEIIAKSGNEYSIDMAFYNSQRKSISERCSLYYSMEGQRIDLIENQ"
BASE COUNT          431 a          213 c          259 g          354 t
ORIGIN      
        1 cgatgatacg cgaaccagtc atgcgctggt gaaacataat tatcagcgcc tgctggataa
       61 aattgatagt ggaagcgacc gttactctcc aatggaatac tggagagcac ttcataaccc
      121 cgactatcgt gatgttcttc agaaaacaat cgttaaacat cccagcgact ggtacttcaa
      181 aaaagacgat gcaatctggc aaccatttct gaatgcgctg aaaaaagatg ctccggaatg
      241 gaagaaatac agcgaagatt ttcttgataa gatggcctgg atgcaggatg tgaccacgga
      301 gaaactgggc cctttgttgt ggcatatgca tccgattatg ttcttaggct gtatgaaaaa
      361 atcgtcagga atatggccac ttgggaaaac ttcagagcgt tatgagtctg gaggaagagg
      421 gccaggtgta atttcatctg gaaaaggaga tcatggcggt gtgtcatatg gaatgtatca
      481 attatcttct aagatgggaa cggttaataa atatattata atgtcaaaat acaaagattt
      541 atttgagggt aaaaaacctg ctacacctga attcaatgaa atatggaaaa aaattgctct
      601 cattcacaaa gaagaattcg cacaagatca gcatcttttt ataaaaaaaa cacactatca
      661 agtacaatta gactatttgg catccaaagg gctaaatttc tctcattctc ttgccgcaat
      721 caatgatatg atttggtcga cttccgttca atatggccct acgactggtt taatttcgag
      781 agcgttgaaa ggaaagaatt tagaaacatt aacagatgca gatgtgataa ctattgttca
      841 ggactacaaa ataaatcatg ttgaagatct ttttccatca tccccgactt ggtgggatga
      901 tttaaagaca cgagcgctaa cagaaaagaa agatttattg gaattaaatg aattgaatct
      961 ggagttaaag aatgaagatt aaattatttg cattgcttat tttcttgccg attatctctt
     1021 ttgcgaaaga accacacgtt tgcaattcct cgatgcagtc tcatccagat attatcgatt
     1081 gcatacaaat tagcacaagg aaaatagagg attccattaa agaaataatt gcgaaatctg
     1141 gcaacgagta ttcaattgat atggcgtttt ataatagcca gagaaagtct atttctgaac
     1201 gatgcagctt atattattcg atggaaggtc aacgaattga tttgatagaa aatcaat
//
LOCUS       DAZGHF010000371         1244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGHF010000371 DAZGHF010000000
VERSION     DAZGHF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.374"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..137)
                     /locus_tag="ACHK3A_005243"
     CDS             complement(<1..137)
                     /locus_tag="ACHK3A_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO8743168.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNSHYSLQETHATPDGYLVK
                     QT"
     gene            245..568
                     /locus_tag="ACHK3A_005244"
     CDS             245..568
                     /locus_tag="ACHK3A_005244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743169.1"
                     /translation="MIDKSVKDAITEYLVSIYTTNLAVAPVDKLTDPRDSVGVVSDLL
                     ISLLISELPDDKRDALMKTASEILRQIEGQQAFAPYAVNIRTRFHSINHLACFIRASK
                     DKPDL"
     gene            complement(546..1037)
                     /locus_tag="ACHK3A_005245"
     CDS             complement(546..1037)
                     /locus_tag="ACHK3A_005245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743170.1"
                     /translation="MIKKYSEHGSKAGEAIAIDEDCLITGDPWLATERDLEGKTFFVR
                     AIKHKDRVVGGIIYSTSTIIPDNYLDISFRPVSIQGCEAYLNYVAELKKINLDYMWPI
                     VQREIMRRKSEGFDVKIEFLSTLMGFLQKPLILFPSLDRNLPVKEGVEKYIVEQVRDL
                     VCL"
     gene            complement(1034..>1244)
                     /locus_tag="ACHK3A_005246"
     CDS             complement(1034..>1244)
                     /locus_tag="ACHK3A_005246"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743171.1"
                     /translation="PSSLAETPNFTSTGKHGMKPAIDSLKTHRKNGTNGKTDFFYLSQ
                     QLVHGINASVKKLRDIELAIIGRTG"
BASE COUNT          301 a          287 c          276 g          380 t
ORIGIN      
        1 gtttgtttaa ccagataccc gtcaggtgtg gcgtgcgttt cctgcaggct gtagtgcgag
       61 ttgctgcgtg atgcgctggt tgagaaacgg gctgcgtggc gttctgtgcc agtgcggaag
      121 ctgcgtctct gtgtcatatc ggaaaacccc gtttaagtta aatgaaaatc agtgccggga
      181 tgtttaacca cgcccggcgc gtggtattct ggtgtttcca cacaaccaga aaaaggtaac
      241 taacatgatt gataaaagcg ttaaggacgc tatcactgaa tacctggtta gcatttacac
      301 aactaatctt gctgtggctc ctgttgataa gttgactgac ccaagagact cagtgggagt
      361 ggtgtccgat ctgcttattt cgctcctcat tagcgaactt cccgatgata aaagggatgc
      421 attgatgaaa actgcgtcag aaattttgcg tcagatagag gggcaacaag cctttgctcc
      481 ctatgccgtg aatataagga cacgatttca tagtattaat catcttgcgt gctttattcg
      541 cgcctctaaa gacaaaccag atctctgact tgttcaacaa tatatttttc caccccttct
      601 ttgactggca ggttccggtc aagggagggg aaaagaatca atggtttttg taaaaatccc
      661 attaacgtcg ataaaaactc aattttcaca tcgaagcctt ctgattttcg tcgcattatt
      721 tcgcgttgta cgattggcca catgtagtcc aggttaattt tctttagctc cgcaacgtaa
      781 ttaaggtatg cctcgcaccc ctgaatgctt acaggtctaa aagaaatatc cagataatta
      841 tcaggaataa tcgtactggt tgaataaata attccaccca caaccctatc tttgtgcttt
      901 attgctctga caaaaaatgt tttgccttct aaatcccttt ctgttgccag ccaggggtct
      961 cctgttatca ggcaatcctc gtcaatggcg attgcttcgc cagccttgct cccgtgttcg
     1021 ctgtatttct taatcatcct gtcctcccaa tgattgccag ttctatatca cgcagctttt
     1081 ttactgatgc gtttattccg tgaaccagct gctggcttaa ataaaaaaag tcggttttcc
     1141 cattcgtgcc attcttgcgg tgcgttttca gtgagtcgat cgcaggcttc atcccatgct
     1201 ttccggttga tgtaaagttc ggcgtttccg ccaggcttga gggg
//
LOCUS       DAZGHF010000372         1222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGHF010000372 DAZGHF010000000
VERSION     DAZGHF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.375"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            44..1200
                     /locus_tag="ACHK3A_005247"
     CDS             join(44..293,293..1200)
                     /locus_tag="ACHK3A_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFO8743172.1"
                     /translation="MSRKIQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDIFKKSNSVFCTGVAEKYALI
                     EQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGT
                     RRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFA
                     PTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALR
                     SQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNE
                     SLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          358 a          287 c          311 g          266 t
ORIGIN      
        1 gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca gaaaaatcca
       61 acgttactct aaagagttca aagccgaagc tgtcagaacg gttcttgaaa atcaactttc
      121 gatcagtgaa ggcgcttccc gattatctct tcctgaaggc actttaggac aatgggttac
      181 cgccgccaga aaagggctcg gtactcctgg ttcccgcacg gtggctgaac tggaatctga
      241 aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt gagcgagata tattaaaaaa
      301 agcaacagcg tattttgcac aggagtcgct gaaaaatacg cgttaatcga acaatggcga
      361 caacaatttc ccattgaagc gatgtgtcag gtatttggtg tatccaggag cggttattac
      421 aactgggtac agcatgaacc ctcagacaga aaacaaagtg atgagcggct aaaactggag
      481 attaaggtgg cacatatccg cactcgcgaa acatatggaa cccggcggct ccagacggag
      541 ctggcagaga atggcatcat cgttggtcgt gaccgactgg cacgtcttcg taaggagcta
      601 aggctacgct gtaagcagaa acgcaagttc agagcgacta cgaactcgaa ccacaatctg
      661 ccagttgcgc caaatctgct gaaccagacg ttcgctccta cagcaccaaa tcaggtctgg
      721 gtggcggacc tgacgtatgt tgccacacag gagggatggt tgtacctcgc tggcatcaaa
      781 gatgtttata cgtgcgaaat tgtcggctac gccatgggag agcgcatgac aaaagagctg
      841 acaggtaaag ccctgtttat ggcgctcagg agccagcgcc cacctgccgg gctaatccac
      901 cactccgatc gtggttcaca gtactgcgca tacgattacc gggtcataca ggagcagttt
      961 ggtctgaaaa catcaatgtc gcgtaaaggt aactgttacg acaacgctcc gatggaaagc
     1021 ttctggggaa cgctgaaaaa tgagagcctg agccactatc gttttaataa ccgggatgaa
     1081 gccatctcag taatacggga atacattgag attttctaca atcgtcagcg tcgtcactct
     1141 cgtctgggga atatctcccc ggcagccttc agggaaaaat atcatcagat ggctgcttaa
     1201 aaaaagaaca aatggtagtg tc
//
LOCUS       DAZGHF010000373         1220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGHF010000373 DAZGHF010000000
VERSION     DAZGHF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.376"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1220
                     /locus_tag="ACHK3A_005248"
     rRNA            <1..>1220
                     /locus_tag="ACHK3A_005248"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          339 a          258 c          383 g          240 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact
      601 aagtgttgtt gggtagggga gcgttctgta agcctgtgaa ggtgtactgt gaggtatgct
      661 ggaggtatca gaagtgcgaa tgctgacata agtaacgata aagcgggtga aaagcccgct
      721 cgccggaaga ccaagggttc ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa
      781 ggcgaggccg aaaggcgtag tcgatgggaa acaggttaat attcctgtac ttggtgttac
      841 tgcgaagggg ggacggagaa ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt
      901 gtaggctggt tttccaggca aatccggaaa atcaaggctg aggcgtgatg acgaggcact
      961 acggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat
     1021 caaatcgtac cccaaaccga cacaggtggt caggtagaga ataccaaggc gcttgagaga
     1081 actcgggtga aggaactagg caaaatggtg ccgtaacttc gggagaaggc acgctgatat
     1141 gtaggtgaag cgacttgctc gtggagctga aatcagtcga agataccagc tggctgcaac
     1201 tgtttattaa aaacacagca
//
LOCUS       DAZGHF010000374         1216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGHF010000374 DAZGHF010000000
VERSION     DAZGHF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.377"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(46..279)
                     /locus_tag="ACHK3A_005249"
     CDS             complement(46..279)
                     /locus_tag="ACHK3A_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO8743173.1"
                     /translation="MPGCTARLLPEGPFSRGQAVAVTTAYRNVFTEDDQGTYFRLVIR
                     NAEGQLRWRCWNFESDAGKQLNVWLSSEGLLRQ"
     gene            complement(350..>1216)
                     /locus_tag="ACHK3A_005250"
     CDS             complement(350..>1216)
                     /locus_tag="ACHK3A_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO8743174.1"
                     /translation="HLGHDNNGGIARGATPESSGSYGFVRLEGDLLITEVAGMSLTTG
                     VYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKA
                     SSGNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFG
                     HGSAQHVRAGFRLGSHNDMNFGKGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSS
                     RGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAE
                     GYNGQATLNVTF"
BASE COUNT          257 a          361 c          346 g          252 t
ORIGIN      
        1 gcgtctcccg gagaagatgc ggcctgatgg atgaaatgaa gacgtttatt gcctgagaag
       61 cccttcactg gagagccaca cattaagctg ttttccggcg tcagactcaa aattccagca
      121 ccgccagcgt agctgccctt cggcattacg gataaccagc cggaaatatg ttccctggtc
      181 atcttcagta aacacattgc ggtaagctgt tgtgacggca acagcctgtc cgcgtgagaa
      241 agggccttcg ggcaataaac gtgctgtaca acccggcata tctgttgctc cctgaaagta
      301 aaaagccccg gtcatgatga ccggggcctg aaggggagtg acctgattat cagaaagtca
      361 cattcagcgt ggcctgaccg ttatagcctt cagcgctgct gccgctgacg ctgtgggcat
      421 aaccggcctg aacgcccagg gtgatatttt cccggacacg ggcttccagt ccggcctgca
      481 ggtccagtga cgtgccattc tgggacggtg agaacgtcat cccgctgcct gcggtggagg
      541 tccccacacg catatcaccc cgggagctga aggtgcggat aacggaaggc tgtacccacc
      601 agttcaccgg cagttcacgc acactgtgtt ttgcactgtc acgcaggggg gcacgggatg
      661 aggtgccttt accaaagttc atatcgttgt ggctgcccag acggaagccg gcacgcacat
      721 gttgtgcact gccatgcccg aacttcacat aaccggcgtt atcctggccg tcatccaggg
      781 aaagtccctg ccaggtgtac tgcagttgtg gctccagcat caggttgtca gtgatactga
      841 agggcagacc ggtttccagt gagcccagcc agccccagcc ccgtgcgcgg aagtcgttat
      901 tgcccgatga cgctttcata ctgtggcggg ttccctgtgc cactatgtca gcccacaggc
      961 cggaggaggt gtgtgtcagg ttcatatatc cgcccaggct gccggcatca tcccggaccg
     1021 tgccggcgcg ggagccgtca tcatccttaa catcaacgga agaatggcct gcagcaccat
     1081 acaccccagt cgtcagtgac ataccggcga cctctgttat gagcaggtca ccctccagac
     1141 ggacgaagcc atagctgccg ctgctttccg gcgtggcccc acgggcaata ccgccgttgt
     1201 tatcgtgacc gagatg
//
LOCUS       DAZGHF010000375         1213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGHF010000375 DAZGHF010000000
VERSION     DAZGHF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.378"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..651)
                     /locus_tag="ACHK3A_005251"
     CDS             complement(<1..651)
                     /locus_tag="ACHK3A_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFO8743175.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLA"
     gene            848..>1213
                     /locus_tag="ACHK3A_005252"
     CDS             848..>1213
                     /locus_tag="ACHK3A_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743176.1"
                     /translation="MNTEKQLPSGIESEVKYTLQFWLLSNGKVMTEKDGLCNPFPLNC
                     IESAHPFTPFQQNQWVELDTVGPNGPVKATFLVHSVLYKLKRQGGSLNDAGRPEYGRC
                     IAHWHTDVFVEKKALPDWLR"
BASE COUNT          296 a          339 c          321 g          257 t
ORIGIN      
        1 cgccagagag gttttcccca ttgacggacg ggcagccagc agaaccaggt caccaggctg
       61 caggccacag gtcatttcat ccagacagtc aaatcccgtg ggggtaccgg taacaccacc
      121 ggagccgagc acactttcca gttgctgcag aacggtatca agcgcggatg aaagagaggc
      181 ttccgtgcgt tcccggagcg tcctgttctg cgcaagtgca aacagacgcc gctctgcagc
      241 atccagcaca tcccgtgatg tggcgttcgg tgccgccaca tcagcaatca gggtgtttcc
      301 ggtggcctgt aactgacgca gaacgctttt ctccaccaca atgcctgcat aggccatgat
      361 gttggccgca gaaggtgtgt tcttgctgag ctcggccaga taagcaaagc cgcccagtac
      421 gtccagcctg ccctgatgtt ccaggtgctc tgacagtgta atcaggtcca gtggctggcc
      481 attcaccgcc agttcagaca tggcacggaa aatagcccgg tgctgagcca cataaaaatc
      541 gtcaggactc agcatcagca ccacttcatc ccagcgttca ttctccagca tcagaccgcc
      601 cagaacggcc tgctctgctt cgtatgcgca gagaacagac atttcagcca tggcgccccc
      661 tccccctctg acagccggaa acctggctac gtgaaaaaag gaaaaagggt gccggacgta
      721 cagctgtcgg gaataaaatc ccggtaactg tctgtacgtt ttcagaccgg tgagtttgcc
      781 gcccccggtc tgaaggtacg gacagcattc gttcattcat aacaagttgt tttaaaggaa
      841 aaagtttatg aatacagaaa aacaactgcc gtccggcatt gaatctgaag tgaaatacac
      901 cctgcagttc tggctgctga gtaacggaaa ggtcatgacg gaaaaagacg ggctctgtaa
      961 cccctttccc ctgaactgta ttgaatcggc tcatcctttt actccgtttc agcagaatca
     1021 gtgggttgaa ctggacactg tcgggccgaa tggaccggtg aaagcgacat tccttgtgca
     1081 cagcgttctg tataaactga aacgtcaggg tggctccctg aatgatgccg gccggcctga
     1141 atacggccgg tgcattgctc actggcacac tgacgtgttt gttgagaaaa aagcgctgcc
     1201 tgactggctg aga
//
LOCUS       DAZGHF010000376         1213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGHF010000376 DAZGHF010000000
VERSION     DAZGHF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.379"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..651)
                     /locus_tag="ACHK3A_005253"
     CDS             complement(<1..651)
                     /locus_tag="ACHK3A_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFO8743177.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLA"
     gene            848..>1213
                     /locus_tag="ACHK3A_005254"
     CDS             848..>1213
                     /locus_tag="ACHK3A_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743178.1"
                     /translation="MNTEKQLPSGIESEVKYTLQFWLLSNGKVMTEKDGLCNPFPLNC
                     IESAHPFTPFQQNQWVELDTVGPNGPVKATFLVHSVLHKLKRQGGSLNDGNWPEYGRC
                     IAHWHTDVFVEKKALPDWLR"
BASE COUNT          304 a          335 c          317 g          257 t
ORIGIN      
        1 cgccagagag gttttcccca ttgacggacg ggcagccagc agaaccaggt caccaggctg
       61 caggccacag gtcatttcat ccagacagtc aaatcccgtg ggggtaccgg taacaccacc
      121 ggagccgagc acactttcca gttgctggag aaccgtatca agcgcggatg aaagagaggc
      181 ttccgtgcgc tcccggagcg tcctgttctg cgcaagcgca aacagacgcc gctctgcagc
      241 atccagcaca tcccgtgacg tggcatttgg tgccgccaca tcagcaatca gggtgtttcc
      301 ggtggcctgt aactgacgca gaacgctttt ctccaccaca atgcctgcat aggccatgat
      361 gttggccgca gaaggtgtgt tcttgctgag ctcggccaga taagcaaagc cgcccagtac
      421 gtccagcctg ccctgatgtt ccaggtgctc tgacagtgta atcaggtcca gtggctggcc
      481 attcaccgca agttcagaca tggcacggaa aatggcccgg tgctgagcca cataaaaatc
      541 gtcaggactc agcatcagca ccacttcatc ccagcgttca ttctccagca tcagaccgcc
      601 cagaacggcc tgctctgctt cgtatgcgca gagaacagac atttcagcca tggcgtaccc
      661 tccctctctg actgccggaa acctggctac gtgaaaaaag gaaaaagggt gccggacgta
      721 cagctgtcgg gaataaaatc ccggtaactg tctgtacgtt ttcagaccag taagtctggc
      781 aaccccggtc tgaaagtaca gacagcattc gttcattcat aacaagttgt tttaaaggaa
      841 aaagtttatg aatacagaaa aacaactgcc gtccggcatt gaatctgagg tgaaatacac
      901 cctgcagttc tggctgctga gtaacggaaa ggtcatgacg gaaaaagacg ggctctgtaa
      961 cccctttccc ctgaactgta ttgaatcggc tcatcctttt actccgtttc agcagaatca
     1021 gtgggttgaa ctggacactg tcgggccgaa tggaccggtg aaagcgacat tccttgtgca
     1081 cagcgttctg cataaactga aacgtcaggg tggctccctg aatgatggca actggcctga
     1141 atacggccgg tgcattgctc actggcacac tgacgtgttt gttgagaaaa aagcgctgcc
     1201 tgactggctg aga
//
LOCUS       DAZGHF010000377         1204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGHF010000377 DAZGHF010000000
VERSION     DAZGHF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.380"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            162..>1204
                     /locus_tag="ACHK3A_005255"
     CDS             162..>1204
                     /locus_tag="ACHK3A_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO8743179.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNLFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNA
                     ESELYSVGLSRVQT"
BASE COUNT          296 a          293 c          324 g          291 t
ORIGIN      
        1 ttacccggtt gcatcatgat gcctgattat atttcccgcg tgtcccggag gttcgggtga
       61 agagaatatt atctttctgt tttcttttct ctgtccacaa cgtgttcgct tctccactgc
      121 cggccctcct gagggatgct ctcattcatg atccatcagt gatggaggca agggcagaag
      181 ccacctctgc ccgttcccgt gtggagcagg ctcgttcagc acactggccg gtcgtcacag
      241 caacaggcag taaactcctt tcccagagtc accgctacga ctatgactac gactctgaag
      301 atattttgcc cggtatccgc ggtgaaatga atctgtttgc ttcgggagct attgaggcgg
      361 atgtcagccg gagtgagtcg gaagccgagt attaccgtta taaagttgat gaggctggcg
      421 aggaaacaat tcgctccttt gtttcattat atcttgatgc gctaagggaa aagcaatcca
      481 ttgcggtact tgaacagagt ctctcccggc ataacgcaat tcttaatgat cttaacacca
      541 tcagtattca cgataccggg cgggaatctg aatttgttca ggccgaagcc cgcaggctga
      601 tggtccggca gcagataaat acccgcaccc gagtgctcaa aaccacgctg ggaaaacttt
      661 cgacctggac cacaaatccg gtaaccgaag ccgatcttga aaatcctttt tcccggatga
      721 cagagtccag attgttaact gattttacgc agacgcctca gaaagggaat ccgtcgtggc
      781 ttgccagtca ggctgatgta gagagtaaaa aagcggcgct gaaagcgcag gagcttgccc
      841 ggtatccccg ggtggattta acgggatcag tgacccggga tgacaggcag ataggtgtca
      901 gtctgtcctg ggacctgttc aaccgtaatg ccagttatgg tgtcagtgaa aaggccgcgc
      961 agatagctgc agctaccgga cgactggact ctgttgcccg aatgattgat gaaaccggac
     1021 gattatcgct gataacggtc agacaaagtc ggatggaaat ggaaacgctg agacgtcagg
     1081 agcaggcctc tgccagagtt gtggactttt atcgtcttca gttcagggtg gcaagaaaga
     1141 cactgattga attactgaat gcagaaagcg aactgtacag tgtcgggctt tcccgggttc
     1201 agac
//
LOCUS       DAZGHF010000378         1201 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGHF010000378 DAZGHF010000000
VERSION     DAZGHF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.381"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..409
                     /locus_tag="ACHK3A_005256"
     CDS             <1..409
                     /locus_tag="ACHK3A_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8743180.1"
                     /translation="FQKTTLSCIEDLKNLGSLIALIAKAAPDTTLANDIESCAGMAWD
                     IINKVSHTLSLLIHKQNISDCPHEIRTQRESCGLSTAELATLLGVDEETIIQWENGKA
                     EPLASQVIPLANILDCDPMWLLTSFHSDTTKES"
     gene            411..602
                     /locus_tag="ACHK3A_005257"
     CDS             411..602
                     /locus_tag="ACHK3A_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743181.1"
                     /translation="MKMFKGLTNEPKTAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFMEQRT"
     gene            599..799
                     /locus_tag="ACHK3A_005258"
     CDS             599..799
                     /locus_tag="ACHK3A_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743182.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCLIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            974..>1201
                     /locus_tag="ACHK3A_005259"
     CDS             974..>1201
                     /locus_tag="ACHK3A_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743183.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTA"
BASE COUNT          397 a          246 c          259 g          299 t
ORIGIN      
        1 gttccaaaaa actacgctat catgcattga agatttaaaa aatcttggct ccctcattgc
       61 tcttatcgca aaagcagcgc cagatacaac tcttgccaat gatatcgagt catgcgccgg
      121 aatggcgtgg gatataataa acaaagtatc tcacacatta tctttgttaa ttcataaaca
      181 aaacatttct gattgccccc atgagatccg cacccaacgc gaaagctgcg gtctatccac
      241 cgctgaactc gccacgctgc ttggagttga tgaagaaacc atcatccagt gggagaacgg
      301 caaagctgaa cctctggcaa gccaggtgat tcctctggca aatatacttg actgcgatcc
      361 gatgtggtta cttacaagtt tccattctga cacgacaaag gaatcgtaag atgaaaatgt
      421 tcaaaggcct caccaacgaa ccgaaaacag cttttcatca cattgcagta ctgcttgaag
      481 cggggttaat catttcggct tccggtgatg aagaatgtga tgaactttcg gatgacatct
      541 ttttactggc acaacaatac gccagaagcg catgcgatgc atttatggaa caaagaacat
      601 gaaaactccc ctgattatgc ttgaggaagt ggcagcagaa ataaaagaaa acacgtcaat
      661 gcttgagttt atatttaaaa actctggtga taacggagag acagatgatt tcttactttg
      721 tctgattcgt tccatgaata aaacctgtga aaaggcttac gagtatgttg atgctctgag
      781 aacgaataaa ggaaattaaa cagacaatca tacataaaaa catgacggtc tacggtcggg
      841 agttctcact attttaaaat ggggatgaca catgaaagac aaacatgcct ataaaaccgc
      901 tttattaatg gctgatgccg gatactggtc agttgcagta ttgtttctca aaaaggcata
      961 cgggaaataa caaatgaaag atgaatacat ttctgcatat cgtcgccgca taacggttgc
     1021 agcactgcgg cggatgaaac gaaaaacagg aagtaatctc ctcactgtaa agcgtcatga
     1081 tggagagtta acaactattg aaataacaga acagttcata agccagttgc tattacgatt
     1141 tgaaggcgtt acccgtggtg agtttgggcg gaaagatggt gaaacagcaa tacgcactgc
     1201 a
//
LOCUS       DAZGHF010000379         1193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGHF010000379 DAZGHF010000000
VERSION     DAZGHF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.382"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            31..747
                     /locus_tag="ACHK3A_005260"
     CDS             31..747
                     /locus_tag="ACHK3A_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8743184.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          392 a          213 c          238 g          350 t
ORIGIN      
        1 tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa tattgttgct
       61 aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg tgtagaaatc
      121 tatgctgctg ccaaacaagg tgaacccgat ccagaattaa acacatcttt aaaattcgtt
      181 attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc aattgataaa
      241 gccaaaggcg gcggagatga aacgttcgta cagggacgtt atgaaggctt tggtcctaat
      301 ggctcaatga ttattgctga gacattgact tcaaatgtta accgtacgat tgctaacgtt
      361 cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt cagctatatg
      421 tttgacaata cgggtgtgat tgtatttaaa ggaacagacc ctgaccatat ttttgaaatt
      481 ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa cattgttatt
      541 tatactgaac ctaccgacct tcataaagga atcgcggctc taaaagcagc tggaatcact
      601 gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct ttccccagaa
      661 gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga tgtacaaaaa
      721 gtttatcata acgtcgcaaa tctctaatga tattttaaag aaatctgtct ttatggcaga
      781 tttctttaat ctcatataat tcttataaag aatataatat ccagctcgtc atattgatta
      841 taaaacccac gctcccagag aaataatatt tattaaaatt ccaggccttc tttttctgat
      901 tacagaaggc aaagtggcaa ttacgtatag tttcccgata aagacgtgac agcaacatcc
      961 cgcataaggc atttttctct ttatctttgt acggtacttc acggaacgaa gtttttgacc
     1021 ttgcgaatcg tgatgtctgt tggggggcaa tttgctcact gaagcgtgag actcgattaa
     1081 acgaacgaaa cacagaaatc aaaaaacccg gtcacttttt ttacaaagtg accgggtaaa
     1141 aataattttt atttttttaa ctgtcctgag actcatagag atgtctcaaa act
//
LOCUS       DAZGHF010000380         1187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGHF010000380 DAZGHF010000000
VERSION     DAZGHF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.383"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..295
                     /locus_tag="ACHK3A_005261"
     CDS             <1..295
                     /locus_tag="ACHK3A_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO8743185.1"
                     /translation="HYPYLRRMSWAQITAAAEDAEIHHDYARALILWQHAYHAATLTL
                     NKNLAVAKIDFCIKRIRMHEQMSRIIRKTGTDEILFRLSKHHHLYEKKKTKEG"
     gene            complement(683..1105)
                     /gene="subB"
                     /locus_tag="ACHK3A_005262"
     CDS             complement(683..1105)
                     /gene="subB"
                     /locus_tag="ACHK3A_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO8743186.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKVLTSNALVGLSTCATSTECFGPDRKKN"
BASE COUNT          350 a          283 c          256 g          298 t
ORIGIN      
        1 gcattatccg tatctgcgcc ggatgtcctg ggcgcagata acggcagcag ctgaagatgc
       61 ggaaatacat catgattatg cccgggcatt aatattgtgg cagcatgcat atcatgctgc
      121 cacgctgact ctcaacaaaa acctggcagt tgctaaaatc gacttctgta ttaaacgtat
      181 ccggatgcat gaacaaatga gcaggataat ccggaagact ggcacagacg aaatactctt
      241 ccgtctgagt aaacaccatc atttatatga gaaaaagaaa acaaaagagg ggtgagaaac
      301 ccattgtatg gggaaaactt gccatgctca ctgcgggggc atttttgtac tggctgttct
      361 aaccgggtaa cccgttgata cacatagtac aataatgttt tatgcaggct tacgcgccag
      421 actgccatca gtagtgcatt gcgtgccagc tcgcggacag gggcagtgac cagccaccac
      481 atgagtaagc atcatttcag cgccatcagg cagctcctga aactcctgcg agttgaaaaa
      541 gcatcctcct gcattagcaa tactggcttg tcagtgccag tgtgatataa gacgtaatgt
      601 caaagttcgt ggtgttgaca tatttctgtc gcaaccaaag tatgctcccg cccagtatat
      661 atctgggcag gagcatcatt tattagttct ttttcctgtc ggggccaaaa cattctgtcg
      721 atgtggcgca ggttgataac ccaacaaggg cattggacgt taataccttc acaaagggcg
      781 gatacgtcca taccccgggt tcataataaa tcctgaccaa ctgacctgtt gtgtaaaaat
      841 atagtgcctg attatataat gtggaaaacg aaggccccca gacagatgaa ttcttcatcg
      901 agcaggctct tatggaagaa ccaccaggtt gtttcccttc aatacaaaaa taagggttat
      961 tatctatttg ccctgtatga aactgatcaa taacaacgcc tgaaaacatg ccatcccggg
     1021 catcgccagt ccactcagcc atggcaggat taagggataa acatcccata acacctgcac
     1081 atacaaaaaa cctcttaatc gtcatattca cctccggaat tttacagttc ttcactcatc
     1141 cttccctgac ggacaggaat ataatttttc ttgcagaaca tgctaat
//
LOCUS       DAZGHF010000381         1178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGHF010000381 DAZGHF010000000
VERSION     DAZGHF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.384"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..852
                     /locus_tag="ACHK3A_005263"
                     /pseudo
     CDS             1..852
                     /locus_tag="ACHK3A_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            862..1178
                     /locus_tag="ACHK3A_005264"
                     /pseudo
     CDS             862..1178
                     /locus_tag="ACHK3A_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          383 a          220 c          291 g          284 t
ORIGIN      
        1 gactgccatt cttcagatga taaagacgtt attgcaattg atggaaaaac gctccggcat
       61 tcttatgaca agagtcgccg caggggagcg attcatgtca ttagtgcgtt ctcaacaatg
      121 cacagtctgg tcatcggaca gatcaggacg gatgagaaat ccaatgagat cacagctatc
      181 catgaacttc ttaacatgct ggatattaaa ggaaaaatca tcacaactga tgcgatgggt
      241 tgccagaaag ataccgcaga gaagatacaa aaacagggcg gtgattattt attcgctgta
      301 aaaggaaatc aggggcggct taataaagcc tttgaggaaa aattcccact gaaagaatta
      361 aataatccag agcatgacag ttacgcaatt agtgaaaaaa gtcacggcag agaagaaatc
      421 cgtcttcaca ttgtttacga tgtccctgat gaacttattg atttcatgtt tgaatggaaa
      481 ggtctgaaga aattatgcgt ggcagtctcc tttcggtcaa taatagcaga acaaaagaaa
      541 gagcccgaaa tgacggtcag atattatatc agttctgctg atttaaccgc agagaagttc
      601 gccacagcaa tccgaaacca ctgacacgtg gagaataagc tgcactggcg tctggacgtg
      661 gtaatgaatg aagacgactg caaaataaga agaggaaacg ccgcagaatt attttcaggg
      721 atacggcaca tcgctattaa tattttaacg aatgataatg tattcaaggc agggttaaga
      781 cgtaagatgc gaaaagcagc catggacaga aactacctgg cgtcagtcct tgctgggagc
      841 gggctttcgt aatctttccc tgggcggtat atcatccagg actcgattgg gctgaagggg
      901 ggatggaatc tgtaccaata tccgctgaat ccagtgcaac acgttgatcc attagggctt
      961 agtactatga taataggtaa cggccccgtt cctgataatc cttttgggca tgctgcggcg
     1021 gcaaatcgat atggattaat gagttcggga actggggatg agatgggagc ctctgtctct
     1081 gattatttta aaaagatgca accccgcaga gatacctgga tttggataat agatacaaca
     1141 gatgaagaag aacaatgtat gatgaataag gcaataga
//
LOCUS       DAZGHF010000382         1147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGHF010000382 DAZGHF010000000
VERSION     DAZGHF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.385"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1057)
                     /locus_tag="ACHK3A_005265"
     CDS             complement(<1..1057)
                     /locus_tag="ACHK3A_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO8743187.1"
                     /translation="MILVCVILWGIYVAVRALINLNERFIDAVSSPAGIIGLFFGLLT
                     VFAILLRFFIYRRLRKKTAAFEQAVSELVQRERGFNETVNAVIARGIRQEKEQLARRR
                     EEFHTARQKASRAMQRIVDSAWKFKAKTLLAGVTINNWQSKYDQLRKEREAYAAVSEK
                     IAFLNLEDNSDWESVRQQFLDKVALLEKAQEEKEYQAELKRQIREEKERQDELDRRQR
                     EAEEEERRLAEQQKLIEEALRAAEGAHREELEKQRLELEQKIQEAHAQYERAKSMAQL
                     TKQGHVYIISNIGSFGEDVFKIGMTRRLEPMDRVKELSGASVPFDFDVHAMISCDDAP
                     ALEKTLHDSLEKYRINRI"
BASE COUNT          280 a          325 c          214 g          328 t
ORIGIN      
        1 taatcctgtt aatgcggtat ttttccagac tgtcatgaag tgttttttcc agcgcaggcg
       61 catcatcaca ggaaatcatg gcgtgaacat caaaatcaaa cggaacagaa gcaccactta
      121 actctttgac cctgtccatg ggttccagcc gtcgggtcat gccaatttta aagacatcct
      181 caccaaacga accaatattc gaaatgatat aaacatgccc ctgtttcgta agctgagcca
      241 tggatttagc acgttcatac tgcgcatgtg cttcctgaat tttctgttcc agctccaggc
      301 gttgtttctc taactcctcc ctgtgagccc cttctgctgc gcgcagcgcc tcctcaatca
      361 gtttctgctg ctcagcaaga cgccgttctt cctcttccgc ttcccgctgg cgccggtcaa
      421 gttcatcctg acgctctttt tcttcacgta tctgacgctt aagctctgcc tgatattcct
      481 tctcttcctg cgctttttcc agcaatgcca ctttgtccag aaactgttgc ctgacactct
      541 cccagtcaga attatcttcc aggttaagaa aggctatttt ctcactgaca gcagcgtaag
      601 cctctctttc cttacgaagc tggtcatatt tgctttgcca gttattaatc gttacaccag
      661 ccagcagagt cttcgcctta aatttccagg cggaatcaac aatccgttgc attgcacggc
      721 tggccttctg tctggcggta tgaaattcct cacgacgtct ggccagttgc tccttttcct
      781 gtcgtattcc ccgggctatc acggcattga cagtttcatt aaaaccccgc tctctctgaa
      841 ccagctctga aacggcctgc tcaaaggctg ctgttttctt tctgagtctc cggtaaataa
      901 aaaaacgaag taaaattgca aatacagtaa gcaaaccaaa aaacaaacca ataatacctg
      961 ccggactcga tactgcatca ataaagcgct cattaagatt tatcagcgcc ctgaccgcca
     1021 cataaatccc ccagagaata acgcaaacta aaatcaggac aaatcccgct gcattatcat
     1081 cttttttctt cctgccatga ctttccttta ttgtaagcgt acagcgaggg ccgtattgac
     1141 ggggatg
//
LOCUS       DAZGHF010000383         1116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGHF010000383 DAZGHF010000000
VERSION     DAZGHF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.386"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(336..503)
                     /locus_tag="ACHK3A_005266"
                     /pseudo
     CDS             complement(336..503)
                     /locus_tag="ACHK3A_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            653..>1116
                     /locus_tag="ACHK3A_005267"
     CDS             653..>1116
                     /locus_tag="ACHK3A_005267"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8743188.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTL"
BASE COUNT          359 a          237 c          184 g          336 t
ORIGIN      
        1 tactatactt tttattaaca taaactcact acaacgcacc tgaaacctct tgctatatat
       61 atgtcaaccg tttgaattta aaataaaaag agtatcattt ttacttgcat ttcttatcaa
      121 gtcacattca acaacagtaa aaaaacatta ttagaaccat tcaattaaca aaaaaaccaa
      181 catccagctt gcttaatttt tctttattaa acgatattga aaatcaattg ataaaataca
      241 tctaaacaac cttttggggc gcaaaagcat aacatcaaac aaataacaca ccgaaaaaac
      301 tcacaattaa taacctatga tatacatact gtttattatg gttgaataag ccactcgata
      361 tctggtgcta cggaagtgtc cacacggttt agcagcaccc gatacttttt tcaggcttcc
      421 agcaacgatc tttcttcctc cgttgcgatt tccagatcta cagcatcctg cagtggcgca
      481 atatactcac tgaattcctg gatgtagaac tgtgtggtga cggtcttcca gctattcggc
      541 tcctgctgta ttgaagcata ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa
      601 ccccttgtaa ttcattgcca taattgattt aattcacaaa taaaactata acatggtgaa
      661 attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta ccctaaagct
      721 ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc ctacaaagga
      781 acaccttctt ccacactttc tggaacattt aggtaacaac catctggata ttggtgttgg
      841 aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa tggatttgaa
      901 cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta aacataaaat
      961 taaccatgat gtttttgaac cttatcccgc ggcgttacat ggtcaatttg attccatttc
     1021 catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct gtgtaatacg
     1081 caatgcggcg caggccttaa ctgacgatgg aactct
//
LOCUS       DAZGHF010000384         1109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGHF010000384 DAZGHF010000000
VERSION     DAZGHF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.388"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            204..386
                     /locus_tag="ACHK3A_005268"
     CDS             204..386
                     /locus_tag="ACHK3A_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8743189.1"
                     /translation="MTFLNQLMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLIP"
     gene            424..669
                     /locus_tag="ACHK3A_005269"
     CDS             424..669
                     /locus_tag="ACHK3A_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO8743190.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDA
                     ILDELLGAPAVPEPEGIAGDGSASDGGEPTPDSDMMM"
     gene            746..961
                     /locus_tag="ACHK3A_005270"
     CDS             746..961
                     /locus_tag="ACHK3A_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8743191.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            965..>1109
                     /locus_tag="ACHK3A_005271"
     CDS             965..>1109
                     /locus_tag="ACHK3A_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO8743192.1"
                     /translation="MNKQYELVVKGINNYPDKITVTVALEIAGQPSLLSPYVAISLDR
                     TEGA"
BASE COUNT          293 a          238 c          325 g          253 t
ORIGIN      
        1 tgctgattga cagtgtgaag gtggaagtga acaacgcgga tgccgcggca tgataaaaaa
       61 ggccgccagc aatgaaggaa agggatgaac tttgtccact acatgaatgc tggcggctgg
      121 taaaacgacc atgatgacac cgaagtctgc ggtgtttttc tacaaaaata agtaaatcat
      181 gtcaactatc acggtgagaa accatgacat ttctgaacca gttaatgctg tacttctgta
      241 cggcggtctg tgtgctgtat cttctttcgg gtgggtacag ggctgtgcgc gatttctggc
      301 gcaggcagat tgataaaagg gccgcagaga aaatcagcgc cagtcagtca gccggaagca
      361 aacccgaaga gccgctcatt ccgtagtcac tttcttaaca acacctttca acgagaaaat
      421 cctatgtcag aaataaaatc gctggtcact gctgaagcag tgaaggaagt cctgcgctct
      481 gaagaagtca gaagcgcact gaaacagaaa ctccgccaga accttgagtc gcgtcttgat
      541 gcagaagtgg atgccattct ggatgaactg ctgggcgcac cggcagttcc tgagccggaa
      601 ggcattgcgg gtgacgggag tgcttcagat ggcggtgaac ccacacccga cagcgacatg
      661 atgatgtaag catgcgcaag ggaccatcgg tgtgtgtcgg tggtctttat attgttgtga
      721 gcttccggat tgcgggagac ggggtatgta ccagatggaa aaaatcacaa caggtgtgtc
      781 atacaccacg tcagcggtgg gaacgggcta ctggttcctg cagttgctgg acagggtttc
      841 cccgtctcag tgggcggcaa taggcgtgct ggggagtctg ctgtttgggc tgctgacata
      901 cctgactaac ctgtatttca agattaaaga agaccgtcgt aaggcggcgc ggggagagta
      961 gacgatgaat aaacaatacg aactggttgt taaagggata aataattacc cggataagat
     1021 tactgttact gtggcactgg aaattgccgg gcagccgtcg ttgttgtcgc catatgtggc
     1081 gattagtctt gaccgtactg aaggtgcca
//
LOCUS       DAZGHF010000385         1108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGHF010000385 DAZGHF010000000
VERSION     DAZGHF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.389"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            192..>1108
                     /locus_tag="ACHK3A_005272"
     CDS             192..>1108
                     /locus_tag="ACHK3A_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743193.1"
                     /translation="MSPDIISSAVNRFSGWPSNDPLKTDKESMTSPMTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDVLLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESASLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVID"
BASE COUNT          341 a          221 c          205 g          341 t
ORIGIN      
        1 tgttattgtt tcattagtaa taatcactta aatgtatcga tgaaacaggt atgacttagc
       61 gggagtgatt gtttctgagt gaacagagac cactttcgcc tgataaatga aaaatgagcg
      121 atttttgcaa gttagttttt ctatagccga gtacgctcag cgcctcgctt gattacaatg
      181 aggttgctgc tatgagtcct gatattatca gttctgctgt caatcgcttc tcaggttggc
      241 catctaatga tcctctaaaa acagataaag aatcaatgac atcacccatg actgtgcagg
      301 taataaagat gattgccgcc atccttgacc cacatcggat agtcgttgga atcgatacac
      361 tttatacaac gcagcgcgaa acatttacct cagagagtca acagctcatt gaatcagcat
      421 tgcaaacact tcatgtgatg aatttaaata acctggaaac aaatctcgat gaggaaggtc
      481 actatctggc ttttggatat aacgttgaca ctcctgacag aataaccgtt tctgcttata
      541 atgaaaaaaa gcctggctgc tatggccaat gtcttttaat acaggatgac gaaacagact
      601 tatttaacct gataaaagtt aaaatttttg aagccaggca taattatgtc gctcaattga
      661 tgagcgatcc ggaatttatg ttcaaattta gttatagcgc tcaacaaaat agattagaac
      721 ctcatcttct ggttcccact tatttccctc taaaaacaaa cgaaccggtt acgcaagagg
      781 atgtactgtt actttatcgt ttctttaaaa tggatggtga ttttaatata ttaacatcag
      841 atgaatacat gagtatcatg acacctctga tgaagtgtga gcgcagcatc cacgaccaca
      901 acaggtatgt gacgggcaaa gatacccttc ttctggacta tcctccttct ggtaaccaga
      961 tatatttcca tatttttcct gacgaatcag catcactagt cctttatttt tcaaacaatg
     1021 acattgaatg tttcgtcttt gaaagagata ttccgagcca gtatcgcttt ttcaaaatgt
     1081 ttaccaatct ggcactggtt attgatca
//
LOCUS       DAZGHF010000386         1094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGHF010000386 DAZGHF010000000
VERSION     DAZGHF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.390"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(79..>1094)
                     /gene="ompC"
                     /locus_tag="ACHK3A_005273"
     CDS             complement(79..>1094)
                     /gene="ompC"
                     /locus_tag="ACHK3A_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO8743194.1"
                     /translation="AEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYARLGFKGET
                     QINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGRNYGVAYDI
                     GAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQYQGKNDRTD
                     VTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQVIYGNNSLNASGQNAEVWAAGLK
                     YDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQYQFDFGLRPSVAYLQSKGKD
                     LGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDKSDFTKASGVATDDIVAVGMV
                     YQF"
BASE COUNT          303 a          246 c          236 g          309 t
ORIGIN      
        1 ctaaaggaag tggcttgagg gagccactta tatgttgggg aggcaaagcc tcccgcaaca
       61 tatcttttag taatcaaatt agaactggta aaccatacct acagcaacga tatcatcggt
      121 agcaacgcca gatgctttcg tgaaatcgct cttatcaatc aggttgattt tgtaatcaac
      181 aaaagtggac atatttttgt tgaagtaata ggttgcacct acatcaatat attcaaccag
      241 gtcctgatca ccccacgcac ccaagtcttt tcctttagat tgcaggtaag caacggacgg
      301 acgcagaccg aagtcgaact gatattgtgc aactacttcg aagttttgtg ctttgttggc
      361 aatatggtta ttaccaaaaa cagtcatgtt ctgggtttca gaataggtgg tagccagata
      421 gatgttgttc gcatcatatt tcagaccagc tgcccatact tcagcatttt gaccagatgc
      481 attcaggctg ttgttaccgt agataacctg attattagtg cggtcagatt tagcataggt
      541 tgcacctaca ccgaatcctt catactcata agtagtggag aaaccgaaac catcaccatt
      601 agcttcagtt acgtcagtgc ggtcattttt accctgatac tgagcagcaa agttcagacc
      661 atcaaccaga ccaaagaagt cgttgttacg ataagttgca acaccagtgg tgcgaccagt
      721 catgaacaca tctgtttggg tccaggtatc gccaccgaat tctggcagaa cgtcagtcca
      781 cgcaccgatg tcatatgcta caccgtagtt acggccgtaa tcgattgagc cgtagtcccc
      841 gaatttcagg cctgcaaatg caagacgggt tttgtctttg gaggagcctt gagattcagc
      901 gcggttgcct ttgaattcat attcccactg accgaaacca gtcagttgat cgttgatttg
      961 ggtttcacct ttgaagccaa gacgggcata agtagtatca ccatcatctg catcattaga
     1021 ggagaagtag tgcttggcat taactttccc atatagatcc agcttgttac tgtctttatt
     1081 ataaatttca gctg
//
LOCUS       DAZGHF010000387         1076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGHF010000387 DAZGHF010000000
VERSION     DAZGHF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.391"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            41..406
                     /gene="cdiI"
                     /locus_tag="ACHK3A_005274"
     CDS             41..406
                     /gene="cdiI"
                     /locus_tag="ACHK3A_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease toxin immunity protein CdiI"
                     /protein_id="HFO8743195.1"
                     /translation="MNKYLFELPYERSEPGWTIRSYFDLMYNENRFLDAVENIVNKES
                     YILDGIYCNFPDMNSYDESEHFEGVEFAVGYPPDEDDIVIVSEETCFEYVRLACEKYL
                     QLHPEDTEKVNKLLSKIPS"
BASE COUNT          340 a          227 c          203 g          306 t
ORIGIN      
        1 caggaattgt aagtaacttc cacccgaaat aggagtaact atgaataaat atttatttga
       61 attgccatat gaacgttctg aacctgggtg gaccataagg tcatactttg accttatgta
      121 taatgaaaat aggttcttgg acgctgtaga aaatatagtt aacaaagaat cttatattct
      181 ggatggaatt tattgtaact ttcctgatat gaatagttat gatgaaagcg aacattttga
      241 aggtgttgaa tttgctgtcg gctatccccc agatgaagat gatatagtga tcgtaagcga
      301 agaaacctgt tttgaatatg ttcgtctggc ctgtgaaaaa tatcttcagt tacaccctga
      361 agatactgaa aaagtaaaca aattactgtc taaaatccca tcctgagtaa agattccggc
      421 cactgcgccg ggatctttct ttaaccgtca ggctcatcca caatgtccat ccccacgctt
      481 aatcaccggc acatctctaa tgtcgttaag cgcaccgggg aaggcgttaa accacgtgag
      541 catctgcggc ctgttgacat acgttgtgtc gttacgggga agtcatcttc acagcgattt
      601 tcagtcagtt agcattaccg acattcattc atcaaattac cggagataat tgatgtcaaa
      661 accgtaagcg taaactgacc gccgtatgta gccatcagac gagaattggt aacttagacg
      721 cccatctgat atagacggac atctaagtat ggaattacag gactggcgaa aagaacctcg
      781 taaaaactat tcgaatgaat tcaaacttcg tatggtggaa ctggcatcac aacctggagg
      841 tttatcccaa gaatattgcc tttgatatgc tggcacatac aatccttgac cattctcaca
      901 gatatactcc cacacacttt tgggttgcgc cccgaaaaca tctttgattt ccatataacc
      961 ccttgattta ataccattta tcaaaagttt aacatttcgg aattttacca actatggaag
     1021 acttagcaat aaccatcaaa caatactaac taatcatccc ttagcaaggg ctaact
//
LOCUS       DAZGHF010000388         1068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGHF010000388 DAZGHF010000000
VERSION     DAZGHF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.392"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..250
                     /locus_tag="ACHK3A_005275"
     CDS             <1..250
                     /locus_tag="ACHK3A_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743196.1"
                     /translation="IKGVRHSIALAQKLVGDTVKLGNEGINVLTMLTDLADVVEELAD
                     ITASHTHPKTGTSPQAAQFSQVAQECRQLKNKYSPIIE"
     gene            668..1003
                     /locus_tag="ACHK3A_005276"
     CDS             668..1003
                     /locus_tag="ACHK3A_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743197.1"
                     /translation="MQGFAKVCKNSGFLRIPKNFNALNNKRFLKLRHDRRSEKGSGDE
                     NGKIRKTRTGAAWRGVAAKITFAHFCTTAPKKDGEGEGKQPDHPLHVTDVKGKLRAGA
                     FRLTAGRNT"
BASE COUNT          318 a          274 c          263 g          213 t
ORIGIN      
        1 gattaagggc gtccgtcaca gtattgcgct ggcgcagaag ctggttggcg atacggtgaa
       61 gctgggaaac gagggcatta atgtactgac catgctgacg gaccttgccg atgtggtgga
      121 agagctggca gatattacgg catcacatac ccaccccaaa acgggcacgt ctccgcaggc
      181 cgcacagttc agccaggtcg ctcaggagtg ccgccagctt aaaaataaat attcaccaat
      241 aatcgagtaa ttattcactt taacaggccg cgcaatgtgg ccttttttat tcccccgaca
      301 ataaaaaata ttcatcaccg gacatcaccc gccacgaaaa aaccgtccgc agcagaaaaa
      361 aagatggacg aaacgcccca cgtaatcccc acgtaaaacg tcgcgtaacg ccacgtaacg
      421 aagaaataat cccgccagcc cgattcgatc ccctgacatc agaaaacgat ttctgcgccc
      481 caaaatcgcc gcaaatggcc ggaaattttc gggggcgacg taacgcgtaa agtgacgtaa
      541 tcggcgctac cccgcacacg cctccggtat ttgtgtacga taaattttgc aaatcgaaag
      601 gggtgcaaaa cacccctcca gggcgcgcca ttactgagtt tttcccttct gaatggtttt
      661 gcactttgtg caaggttttg caaaagtgtg caaaaactcc ggctttctga ggatcccgaa
      721 aaattttaat gcactgaata acaagagatt tttaaaatta cgtcacgatc gaagatctga
      781 aaaaggatcc ggtgacgaaa acggaaaaat caggaaaacc agaactggcg cggcctggcg
      841 gggagtggca gcaaaaatca cttttgcaca cttttgcaca actgctccga aaaaggatgg
      901 cgaaggggaa ggaaaacagc cggatcatcc gttgcacgta acggacgtaa aaggtaaatt
      961 acgcgcgggt gcctttcggc tgacggctgg aaggaatacc tgaaggccgg atgtgaaaag
     1021 gccccgagtc aactaacgtt aacccgaggc cctgacactt cctacctg
//
LOCUS       DAZGHF010000389         1068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGHF010000389 DAZGHF010000000
VERSION     DAZGHF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.393"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..250
                     /locus_tag="ACHK3A_005277"
     CDS             <1..250
                     /locus_tag="ACHK3A_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743198.1"
                     /translation="IKGVRHSIALAQKLVGDTVKLGNEGINVLTMLTDLADVVEELAD
                     ITASHTHPKTGTSPQAAQFSQVAQECRQLKNKYSPIIE"
     gene            666..956
                     /locus_tag="ACHK3A_005278"
     CDS             666..956
                     /locus_tag="ACHK3A_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743199.1"
                     /translation="MQGFAKVCKNSGFLRIPKNFNALNNKRFLKLRHDRRSEKGSGDE
                     NEKIRKARTGAAWRGVATKITFAHFCTTAPKKDGEGEGKTATSSVARNGRKR"
BASE COUNT          319 a          275 c          262 g          212 t
ORIGIN      
        1 gattaagggc gtccgtcaca gtattgcgct ggcgcagaag ctggttggcg atacggtgaa
       61 gctgggaaac gagggcatta atgtactgac catgctgacg gaccttgccg atgtggtgga
      121 agagctggcc gatattacag catcacatac ccacccaaaa acgggcacgt ctccgcaggc
      181 cgcacagttc agccaggtcg ctcaggagtg ccgccagctt aaaaataaat attcaccaat
      241 aatcgaataa tcattcactt taacaggccg cgcaatgtgg ccttttttat tcccggacga
      301 taaaaaatat tcatcaacgg acatcaccca ccacgaaaaa ccgtccgcag cagaaaaaaa
      361 gatggacgaa acgccccacg taatccccgc gtaaaacgtc acgtaacgcc acgtaacgaa
      421 gagataaccc cgccagcccg attcgatccc ctgacatcag aaaacgattt ctgcgcccca
      481 gaatcgccgc aaatggccgg aaattttcgg gggcgacgta acgcgtaaag tgacgtaatc
      541 ggcgctaccc cgcacacgcc tccggtattt gtgtacgata aattttgcaa atcgaaatgg
      601 gtgcaaaaca ccccgccagg gcgcgccatt actgaggttt tcccttctga atggttttgc
      661 actttgtgca aggttttgca aaagtgtgca aaaactccgg ctttctgagg atcccgaaaa
      721 attttaatgc actgaataac aagagatttt taaaattacg tcacgatcga agatctgaaa
      781 aaggatccgg cgacgaaaac gaaaaaatca ggaaagccag aactggcgcg gcctggcggg
      841 gagtggcaac aaaaatcact tttgcacact tttgcacaac tgctccgaaa aaggatggcg
      901 aaggggaagg aaaaacagcc acgtcatccg ttgcacgtaa cggacgtaaa aggtaaatta
      961 cgcgcgggtg cctttcggct gacggctgga aggattacct gaaggccgga tgtggaaagg
     1021 ccccgagtca aacatttgtt taacccgagg ccctaaccca tctacctc
//
LOCUS       DAZGHF010000390         1055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGHF010000390 DAZGHF010000000
VERSION     DAZGHF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.394"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..567
                     /locus_tag="ACHK3A_005279"
     CDS             <1..567
                     /locus_tag="ACHK3A_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFO8743200.1"
                     /translation="AEDTDPVTNKNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDF
                     IGLDAAVADLVHTLIDESYRGHPDLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIG
                     RQIAGLTAYTPPFMPEGRLIVTTLANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEG
                     YAVEHDEIYAAYDKLTIKGAEAVPGVGG"
     gene            585..>1055
                     /gene="gpM"
                     /locus_tag="ACHK3A_005280"
     CDS             585..>1055
                     /gene="gpM"
                     /locus_tag="ACHK3A_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO8743201.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYITDGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRNVTT"
BASE COUNT          227 a          341 c          321 g          166 t
ORIGIN      
        1 gccgaagata ccgacccggt aaccaacaaa aacggcgagg acgtcaacac cggctggcat
       61 gccatcgtca aaggccgcag cgcaaaacag atcgtggagg ctggcccgac gctgggcaag
      121 ggtggcgact ttatcgggct ggatgcagcc gtggcagacc tcgtgcatac cctcattgat
      181 gagtcatatc gcggtcatcc ggatctggtg gtactggttg cgcccgacct tgtggcacag
      241 gacaaggtga cgctgcttaa cgccatcgac gcccccacgg aaaaagtggc cgcacagctt
      301 atcggtcgcc agatcgccgg gctgacagca tacaccccgc cgttcatgcc ggaagggcgc
      361 ctgattgtca ccacgctggc aaaccttcac atctacacgc agcgcggcac ccgtcagcgc
      421 cgtgcggagt ggaacgacga tcgcaagcgt tttgaaaaca actatctgcg catggaaggt
      481 tacgccgtgg agcacgacga aatttacgcg gcctacgaca aactgaccat caagggcgca
      541 gaagccgtac caggcgtcgg cggctgagga cggagggcgg cgcaatgaca ccctgccagc
      601 ggcaccgggc gaaaatccgc acacaggagg cactcgaacg ccgggaggcg ctgacggcct
      661 ccccggtcag cttccacctg ttacgcgcgg agcttgatcg cgatgtggca cggctgcgca
      721 gtcttccggt ccgcgaagag cggctggcgt tcaagcgcga cattctcctg ccgcgctggc
      781 tgcccgtcgc ggagcgctat atcactgacg gaaagcgcca cgcctgcccg gtactggtgt
      841 actgcatcat ctggctgttc gacacgggcg acctgagtcg cgcccttgac tgggcagaca
      901 ttgccatcag tgaaggccag gccacaccgg acaacttccg cagcaccctg cccgccttcg
      961 tggcagacac ggtgctgcag tgggccaccg acaacagcga ggccggacac agcatagaac
     1021 cgtatttttc gcgcaccttc aggaacgtca ccacc
//
LOCUS       DAZGHF010000391         1055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGHF010000391 DAZGHF010000000
VERSION     DAZGHF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.395"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..567
                     /locus_tag="ACHK3A_005281"
     CDS             <1..567
                     /locus_tag="ACHK3A_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFO8743202.1"
                     /translation="AEDTDPVTNKNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDF
                     IGLDAAVADLVHTLIDESYRGHPDLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIG
                     RQIAGLTAYTPPFMPEGRLIVTTLANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEG
                     YAVEHDEIYAAYDKLTIKGAEEAEDVGA"
     gene            585..>1055
                     /gene="gpM"
                     /locus_tag="ACHK3A_005282"
     CDS             585..>1055
                     /gene="gpM"
                     /locus_tag="ACHK3A_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO8743203.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYIADGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWDTDNSEAGHSIEPYFSRTFRNVTT"
BASE COUNT          228 a          336 c          322 g          169 t
ORIGIN      
        1 gccgaagata ccgacccggt aaccaacaaa aacggcgagg acgtcaacac cggctggcat
       61 gccatcgtca aaggccgcag cgcaaaacag atcgtggagg ctggcccgac gctgggcaag
      121 ggtggcgact ttatcgggct ggatgcagcc gtggcagacc tcgtgcatac cctcattgat
      181 gagtcgtatc gcggtcatcc ggatctggtg gtactggttg cgcccgatct tgtggcacag
      241 gacaaggtga cgctgcttaa cgccatcgac gcccccacgg aaaaagtggc cgcgcagctt
      301 atcggtcgcc agatcgccgg gctgacagca tacaccccgc cgttcatgcc ggaagggcgc
      361 ctgattgtca ccacgctggc aaaccttcac atctacacgc agcgcggcac ccgtcagcgc
      421 cgtgcggagt ggaacgacga tcgcaagcgt tttgaaaata actatctgcg catggaaggt
      481 tacgccgtgg agcacgacga aatttacgcg gcctacgaca agctgaccat caagggcgca
      541 gaagaagccg aagacgtcgg cgcataagca cagagggcgg cgcaatgaca ccctgccagc
      601 gacaccgggc gaaaatccgc acacaggagg cactcgaacg ccgggaggcg ctgacggcct
      661 ccccggtcag cttccacctg ttacgcgcgg agcttgatcg cgatgtggca cggctgcgca
      721 gtcttccggt ccgtgaagag cggctggcgt tcaagcgcga cattctcctg ccgcgctggc
      781 tgcccgtcgc ggagcgctat atcgctgacg gaaagcgcca cgcctgcccg gtactggtgt
      841 actgcatcat ctggctgttc gacacgggcg acctgagtcg cgcccttgac tgggcagaca
      901 ttgccatcag tgaaggccag gccacgccgg acaacttccg tagcaccctg cccgccttcg
      961 tggccgacac ggtgctgcag tgggacaccg acaacagcga ggccggacac agcatagagc
     1021 cgtatttttc gcgcaccttc aggaacgtca ccacc
//
LOCUS       DAZGHF010000392         1027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGHF010000392 DAZGHF010000000
VERSION     DAZGHF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.396"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1027
                     /locus_tag="ACHK3A_005283"
     CDS             <1..>1027
                     /locus_tag="ACHK3A_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFO8743204.1"
                     /translation="VFNVVGENGQVTEAVLTQDGPVLENFTAGQSGTLSDDDRGGFIW
                     LGGLAFGGGLAAMALAAGGGGGSKHRHENHEADTTAPSAPELTAGDNGSVSVELPGDA
                     NKGDTVEITFEDENGDEQKVTLEKGDNGWTSSNPALIPDSQGDTATIAPDTVKDNSEV
                     TAVAKDPAGNESTPVTVTSKTDGVADAPVLTIPEVADSVANAAELKDGLQAEVKLPAG
                     TVEGAVITLTVTHPDQSTENVTHNVTGDEVTAGKVSMDIPEDAVVDGQNSVSVSLTQG
                     SNPAKAGNKVEFVVDGQVPGDTNGDGVADVTPAVAIPEATDGVNAKELKDGVQAEVTV
                     PAGSAEGD"
BASE COUNT          274 a          206 c          325 g          222 t
ORIGIN      
        1 gtatttaatg ttgtgggtga aaatggtcag gtaactgaag ctgtcctgac ccaggatggc
       61 cctgtactgg agaatttcac tgcaggacaa tccgggacct tgtcagatga tgatcgtggc
      121 ggttttatat ggttgggcgg tctggcattt ggtggggggc ttgctgctat ggctttggct
      181 gcaggtggtg gtggcggcag caagcacaga catgaaaacc atgaggcaga tacaacagca
      241 ccgtcagcac cggaattaac ggccggagat aatggttcag tttctgttga actgccgggt
      301 gatgcaaata aaggcgatac tgttgaaatc acctttgaag atgaaaatgg tgatgaacag
      361 aaagtcacac tggagaaagg ggataacggc tggacatcca gcaatcctgc actgatcccg
      421 gacagccagg gtgatacagc cacaatcgcc ccagacactg ttaaagataa cagtgaagtc
      481 acagcagttg ctaaagatcc ggctggtaat gaaagtactc cggtcactgt gacgtcaaaa
      541 actgatggtg tggcagatgc tccggtcctg accattccag aagtggctga tagtgtggcc
      601 aatgcggctg agctgaaaga tggtttacag gcagaagtca agttgccggc aggaacagtg
      661 gaaggtgcgg tcattacact gacagtgact catccggatc aaagtaccga aaatgtgaca
      721 cataacgtca ccggagatga ggttactgcc ggtaaggtat ccatggatat tcctgaggat
      781 gctgttgttg acggtcagaa cagtgtgagt gtcagcctga cccagggcag taatccggct
      841 aaagcgggga ataaagtgga gtttgtggtt gacgggcagg ttccgggtga caccaatggt
      901 gatggtgtgg cggatgtcac accggcagtg gccattccgg aagcgacgga cggtgtgaat
      961 gccaaagaac tgaaggatgg cgtacaggca gaagtgacgg ttccggcagg cagtgctgaa
     1021 ggtgata
//
LOCUS       DAZGHF010000393         1020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGHF010000393 DAZGHF010000000
VERSION     DAZGHF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.397"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..268)
                     /locus_tag="ACHK3A_005284"
     CDS             complement(<1..268)
                     /locus_tag="ACHK3A_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO8743205.1"
                     /translation="MRFICDNSYFLEGVKEYINPGKFIISGMQTDNVVIISSSRISEI
                     VGFLYKNNISDMNTLYFSSPEALRFLWGVVDSPVYDIRHLTERCS"
     gene            complement(392..868)
                     /locus_tag="ACHK3A_005285"
     CDS             complement(392..868)
                     /locus_tag="ACHK3A_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO8743206.1"
                     /translation="MQIIIMTRDRYLEYGLMRMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVIFHEYGCTFLADQLGISMKTVCAHLYNAMEKSGLRGVSVKYLCSTADR"
BASE COUNT          289 a          292 c          195 g          244 t
ORIGIN      
        1 ctgaacatcg ctcagtaaga tgtctgatat cataaaccgg actgtcaacc actccccaga
       61 gaaaacggag cgcttccgga gaagaaaaat aaagggtgtt catatcactt atattattct
      121 tatacagaaa gccgacaatt tcagatatcc gggaagaaga tattatcacc acattatctg
      181 tctgcatgcc gctgataatg aatttaccgg gattaatata ctccttcact ccctccagaa
      241 aataactgtt atcacaaata aaacgcatac ccgttccaca tgaaatttat cacgcctgac
      301 cacataacat taattatccg gagcaccggg atgcccgtga cacagcatgc acagaaattc
      361 ccgtcgcaac agccttcatc agaccggctt tttaccggtc tgcggtgctg cagagatatt
      421 taacactgac acctcgcaga ccgcttttct ccatcgcatt gtaaagatgc gcacataccg
      481 tcttcatgct tatccccagc tggtctgcca gaaaggtaca gccgtactca tggaaaataa
      541 ccccgaagac caccaccatc tcggtacggg tcagtggtct tgccgtatgt ccgaacagcc
      601 acgcaccacg acggatggcc tgcctgatat ctgtcaggca tctcacagag gaaacaggaa
      661 tgacaagaaa acgacgccca cagaacatac agtatgtaag cctttccaga ttacggtcac
      721 agagaatcac ataactgtct tcgggaagtg gcagacggcg ctttccggca tcaaacagtt
      781 cacggcctgt cgtgagcctg tatccgttca gcatacgcat caggccatat tccagatatc
      841 tgtcacgggt cataataata atctgcatac tctcagaccc ttatctcccg gataccggct
      901 ccgttcacca ttaccggtat aactgactca tcattcataa cggaaactgt ctgccgcata
      961 catcagtgcc cgctcttcca gctctgacag actcagctct ttcagttccc cttctccttc
//
LOCUS       DAZGHF010000394         1011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGHF010000394 DAZGHF010000000
VERSION     DAZGHF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.398"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1011
                     /locus_tag="ACHK3A_005286"
     CDS             <1..>1011
                     /locus_tag="ACHK3A_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HFO8743207.1"
                     /translation="TSAGQLQSQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSD
                     SGRLTGVHTTTSNLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRY
                     DRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSRHRLVHYTRTQYAEPLVESRYLYDPL
                     GRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRTRIQTVYQPGSFTP
                     LIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSE
                     ESRRWLASCGLTVEQIQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGATEWCAEYD
                     EW"
BASE COUNT          232 a          317 c          314 g          148 t
ORIGIN      
        1 atacctctgc cgggcagtta cagagccagc acctgaacag cctgcagtat gaccgcgatt
       61 acacctggaa cgacaacggc gaactcatcc gcatcagcag cccgcgacag acccggagtt
      121 acagctacag cgactccggc aggctgaccg gcgtccacac caccacctca aatctggata
      181 tccgcatccc gtatgccacg gacccggcag gtaaccgcct gcccgacccg gagctgcacc
      241 cggacagcac cctcagcatg tggccggata accgtatcgc ccgtgacgcg cactatcttt
      301 accggtatga ccgtcacggc aggctgacag agaaaaccga cctcatcccg gaaggggtta
      361 tccgcacgga tgatgagcgg actcaccggt accattacga cagccggcac cggctggtgc
      421 actacacgcg gacacaatat gcagagccgc tggtcgaaag tcgctatctt tacgacccgc
      481 tgggccgcag ggtggcaaaa cgggtatggc ggcgtgaacg ggacctgacg ggctggatgt
      541 cgctgtcacg gaaaccggaa gtgacctggt acggctggga cggcgaccgg ctgaccacga
      601 tacagaacga cagaacccgc atccagacgg tttatcagcc ggggagcttc acgccactca
      661 tcagggtcga aaccgccacc ggagagctgg cgaaaacgca gcgccgcagc ctggcggatg
      721 cccttcagca gtccggcggc gaagacggtg gcagtgtggt gttcccgccg gtgctggtgc
      781 agatgctcga ccggctggaa agtgaaatcc tggctgaccg ggtgagtgag gaaagccgcc
      841 gctggctggc atcgtgcggc ctgacggtgg agcagataca aaaccagatg gacccggtgt
      901 acacgccggc gcgaaaaatc cacctgtacc actgcgacca tcgcggcctg ccgctggcgc
      961 ttgtcagcac ggaaggggca acagaatggt gcgcagaata cgatgaatgg g
//
LOCUS       DAZGHF010000395         1006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGHF010000395 DAZGHF010000000
VERSION     DAZGHF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.399"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            342..599
                     /locus_tag="ACHK3A_005287"
     CDS             342..599
                     /locus_tag="ACHK3A_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO8743208.1"
                     /translation="MLHIIRKGDKTTHGGSVLTASETMKFGGIGVARKGDKVSCPGHG
                     PTIIIEGNPDYLDHGIPVAFHGHKCACGCTLISSFAAAKVA"
     gene            602..>1006
                     /locus_tag="ACHK3A_005288"
     CDS             602..>1006
                     /locus_tag="ACHK3A_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743209.1"
                     /translation="MPVWLDAIPEKAPKIARPNTRRWLLFLAFIMLGGMALTLWCWTS
                     ERTGFVFWFTALGLPFCTWGLIFGLRRFAYKAGQVGAESRNVEREALIDSEILRGQRC
                     AWILGTYIQSPAGNKADDLLEAMEVAAPAIDFS"
BASE COUNT          272 a          203 c          255 g          276 t
ORIGIN      
        1 ggtgaaatac caagggtaat gatttcctca gaaaagaatg tttgtggtat aaaaattaat
       61 gacgagtttg taaatatgta ggtggttaca aatgtgaatt ttattgcgca ctatctgtag
      121 tatcgcatcc atatttgtat gaaattggca aaaaataata gtatttattg ataacccaat
      181 ggtaaatgca tgcgatggag aactctatct atcaataatc agtgacccgg aaacattcat
      241 aataaaaatc cagacaatca ctatcgatgg cagagcccag cataaacatg ggaattaaag
      301 agtgatgaat tagttaagag ataagaaaga ggaatttaat aatgcttcat attattcgta
      361 aaggcgataa aactactcac ggaggttccg ttctgaccgc ttctgaaacg atgaaattcg
      421 gtgggatagg cgtcgcccgt aaaggcgata aagtgtcatg tccgggtcat ggtcctacca
      481 tcatcataga aggcaatcct gattatcttg accatggtat cccggtggca tttcacggtc
      541 ataaatgcgc ctgtggttgc acattgatca gttcatttgc cgcagcaaag gttgcctgat
      601 tatgcctgtc tggctggatg ccatccctga aaaggcccca aagatagcgc gccccaatac
      661 tcgccgctgg ctgttgttcc tggcatttat catgctgggg gggatggcgc tcacattatg
      721 gtgctggacg tcagaacgaa caggatttgt cttctggttc accgctctgg ggcttccatt
      781 ttgcacgtgg gggcttattt tcggtttacg tcggtttgcc tataaagccg ggcaggtggg
      841 ggctgagtcc cgaaatgtcg agcgggaagc gcttattgac agtgaaattc tgcgtggtca
      901 gcgctgtgcc tggatcctcg gtacttatat tcagagcccg gccggaaaca aagccgatga
      961 cctgcttgag gcaatggaag tcgcggctcc tgctattgat ttctcg
//
LOCUS       DAZGHF010000396          982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGHF010000396 DAZGHF010000000
VERSION     DAZGHF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.400"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..263
                     /locus_tag="ACHK3A_005289"
     CDS             <1..263
                     /locus_tag="ACHK3A_005289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743210.1"
                     /translation="KRRTGMEEMQDKMAWMQNLITEKLGPSLWHMHPIAFPGALIKKN
                     TGWAHSKFANLLGSVESENDYSAYNVTKTGKSVPHYNINLTK"
     gene            434..>982
                     /locus_tag="ACHK3A_005290"
     CDS             434..>982
                     /locus_tag="ACHK3A_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743211.1"
                     /translation="MGCNKKSNKFLLFVSCMLLSFVTYSQDSSNPQFSDYLVPVSNGP
                     FEKNIHFNKEQENYSQHWKNAVQEELKKPVNFAGHFRIYTAFGGHGKECLRDNWVCGW
                     VIDKLSGEVVATLPSDNNGSYNYADVSDNGTPVGLPFEIDTYKNSSMIAITGQSISVS
                     KSDNPVCKTTLFNFNNNEYVKLI"
BASE COUNT          330 a          157 c          212 g          283 t
ORIGIN      
        1 aaaaaagacg taccggaatg gaagaaatgc aggataagat ggcctggatg cagaatctga
       61 tcacggagaa actgggccct tcgttgtggc atatgcatcc gattgccttt cctggagcat
      121 taataaagaa aaatactggg tgggcgcata gcaagtttgc aaaccttttg ggaagtgtcg
      181 agtcagaaaa tgattattcg gcatataatg ttactaagac aggtaaatct gtcccgcact
      241 ataatattaa tctcacaaaa tgacattatc tgaggtaatt gatgccccaa gagaacgaaa
      301 aatggtaaaa agaaatacag gttagtacct agggtggcag aagttgaggg ggagtcgtac
      361 tattctggtg atggattgaa taaagctcac ctattaccag atacgatgat taaagctctt
      421 gaggattcta aaaatgggat gcaataaaaa atcaaacaaa tttttgcttt ttgtgtcatg
      481 catgctcttg tcgtttgtaa cgtactcaca agacagttct aatcctcagt tttctgacta
      541 cctggttcct gtaagcaacg gaccatttga aaagaatatt cactttaata aagagcagga
      601 aaattactcg caacactgga agaatgcagt ccaagaggaa ctaaaaaagc ctgttaattt
      661 tgcagggcac tttagaatct atacagcctt tggcggtcat ggtaaagaat gccttcgtga
      721 taattgggta tgtggttggg ttattgataa actatcaggg gaagttgtag caacacttcc
      781 atcagataat aatggaagtt ataactatgc tgatgtgtct gataatggta cccctgttgg
      841 tcttccattt gagattgata cttataaaaa tagttcaatg attgcaataa ccggtcaatc
      901 tatctcagta agcaagagtg ataatcctgt atgtaaaacg actttattta attttaacaa
      961 taatgaatat gtgaaattaa tt
//
LOCUS       DAZGHF010000397          956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGHF010000397 DAZGHF010000000
VERSION     DAZGHF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.401"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            173..802
                     /locus_tag="ACHK3A_005291"
     CDS             173..802
                     /locus_tag="ACHK3A_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743212.1"
                     /translation="MSGNATNEIILAKINDILVDFNELNNQINNFPEKFSGKIDAINF
                     AIKQTPDALEESLKKLFSAAEELEKTVQKSTDECRVSMRAYAINELDNLKVKISEAIN
                     ESIKDSIDMPLDQAKERIVQINKMLQNSTGVEMSTRVAFGIGGLLGLSFLSLAMCISA
                     GYLYFQERSKAERYHNAYREQQIVIERLPPETQRQFRDELGRYLTRNQK"
BASE COUNT          331 a          127 c          227 g          271 t
ORIGIN      
        1 aaattctgtc ctgaatgatg acagggattt tagaacatta atgagggaaa ctgatgatcc
       61 agatatactt gaactattgt ctactgaaag agctataaag cgtcttgcta caggtataaa
      121 taaagagctg gatattgcat ttaatcatct cgaaattata taaggtgtga atatgtctgg
      181 aaatgcaaca aatgaaatta ttttggcgaa aataaacgat attcttgtcg attttaatga
      241 gttaaacaat caaataaata actttcctga aaagttttca gggaaaatag atgcaattaa
      301 ctttgccata aaacagacac ctgatgcgct agaggaatca ttaaaaaaat tattttctgc
      361 ggcagaagag ttagaaaaaa cagtgcagaa aagtacagat gaatgtcggg tatcaatgag
      421 ggcgtatgct ataaatgagt tagataatct gaaagtgaaa atatcagaag ctataaatga
      481 atccattaaa gattctattg atatgccgtt agatcaggcg aaagaacgga ttgttcagat
      541 aaataagatg ctacaaaata gtaccggggt agaaatgagt acaagggtag catttggtat
      601 tggtggattg ctggggcttt catttctttc tttagcaatg tgtatatcag cgggatatct
      661 gtattttcag gagcggagca aagcagagcg ttatcataat gcgtaccgtg aacaacagat
      721 agtgatagaa cgtttgccgc cagaaacgca gcgccagttc cgggatgaat tggggcgtta
      781 tcttacccgt aatcagaagt aaataggcgt tagatggacg ttagatggac attagatgga
      841 cattagatgg atcttagttg gccatctttg atttagatgg acgttagatg gatattagat
      901 ggacgctaga tggacattag ttagctgtca gttacagggg gagattgagg gtaaat
//
LOCUS       DAZGHF010000398          953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGHF010000398 DAZGHF010000000
VERSION     DAZGHF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.402"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..229)
                     /locus_tag="ACHK3A_005292"
     CDS             complement(<1..229)
                     /locus_tag="ACHK3A_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO8743213.1"
                     /translation="MKEYINPGKFIISGMQTDNVVIISSSRISEIVGFLYKNNISDTN
                     TLYFSSPEALRFLWGVVDSPVYDIRHLTERCS"
     gene            complement(325..801)
                     /locus_tag="ACHK3A_005293"
     CDS             complement(325..801)
                     /locus_tag="ACHK3A_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO8743214.1"
                     /translation="MQIIIMTRDRYLEYGLMCMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVVFHEYGFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYLCSTADR"
BASE COUNT          273 a          265 c          180 g          235 t
ORIGIN      
        1 ctgaacatcg ctcagtaaga tgtctgatat cataaaccgg actgtcaacc actccccaga
       61 gaaaacggag cgcttccgga gaagaaaaat aaagggtgtt cgtatcactt atattattct
      121 tatacagaaa accgacaatt tcagatatcc gggaagaaga tattatcacc acattatctg
      181 tctgcatgcc gctgataatg aatttaccgg gattaatata ctccttcatt ccctccagaa
      241 aataactgtt atcacaaata aaatgcatac ccgttccaca tgaaatttat cacgcctggc
      301 cacataacat taattatccc ggatttaccg gtctgcggtg ctgcagagat atttaatact
      361 gacgccgcgc agaccatttt tctccatcgc attgtaaaga tgcgcacata ccgtcttcat
      421 gcttatcccc agctggtctg ccagaaaggt aaaaccgtac tcatggaaaa caactccgaa
      481 gaccaccacc atctcggtac gggtcagtgg tcttgccgta tgtccgaaca gccacgcccc
      541 acgacggatg gcctgcctga tatctgtcag gcatctcaca gaggaaacag gaatgacaag
      601 aaaacgacgc ccacagaaca tacagtatgt aagcctttcc agattacggt cacagagaat
      661 cacataactg tcttcgggaa gtggcagacg gcgctttccg gcatcaaaca gttcacggcc
      721 tgtcgtgagc ctgtatccgt tcagcataca catcaggcca tattccagat atctgtcacg
      781 ggtcataata ataatctgca tactctcaga cccttatctc ccggataccg gctccgttta
      841 ccattaccgg tataactgac tcatcattca taacggaaac tgtctgccgc atacatcagt
      901 gcccgctcct ccagctctga cagactcagc tctttcaggt ccccttctcc ttc
//
LOCUS       DAZGHF010000399          952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGHF010000399 DAZGHF010000000
VERSION     DAZGHF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.403"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            90..>952
                     /locus_tag="ACHK3A_005294"
     CDS             90..>952
                     /locus_tag="ACHK3A_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743215.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLM"
BASE COUNT          281 a          234 c          238 g          199 t
ORIGIN      
        1 agcggctttt tcctggttaa gtcgtcatta tcaggaaaaa cacgcgacat cctgcgcgcc
       61 tacaaaacag caacaaggga gtctgaacga tgaaagaagc tgatctgaaa gcactgcgtg
      121 acgccgcaca aaataccgta tatggcgaca agggatgtga tctggaagca tttcactccc
      181 ttgccactcc gccagttatc cttgcgctaa tcagtcacat agaaaatctg gagaaaaaac
      241 tggatgaaat cgtaacgtca gagattagcc cgaaggcctg gctgaataac cttacggggg
      301 aagtttgcag tattgatgcc atgcctgacg cccgtaatga cagagaaaca tacaccccgc
      361 tttatcctgc ctggcagcgc attgccgccg cagatgataa cggcagagtg gcatggatgt
      421 atacgcacaa aaaagcaggt tacagggtcc ccacaatgac atcgtgggaa agtcttgcag
      481 aagagcgggg aggctctctg gataaatata tcgtcagtcc actattcact gttcctcaga
      541 taccgccagt gagaacagaa caggatgctt accctgatgt gcaggtttca tgggatgcaa
      601 cacgtcacta tgtcaacggc tggaacgcct gccgacaggc aatgctgacg acggcagaaa
      661 aagtacttga tgtgcgaatc cctgaatata tcggacgctg gctttccgct gcacttcatg
      721 atcagaactc atgcgaggaa atgaaaaacg atatacgacg cttcctggct gaataccacc
      781 atgttccatc gaaatataac ggctggcgac ttgtaccgga caagttaacc gaacagatga
      841 agtaccggat tgtgccacta ctggcgacca tcaagccccg cagcgtcgat gaatatgacc
      901 gctcaggaga aaacgcacaa tttcttgaat gggtatggac gcaactgatg ga
//
LOCUS       DAZGHF010000400          942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGHF010000400 DAZGHF010000000
VERSION     DAZGHF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.404"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            242..898
                     /locus_tag="ACHK3A_005295"
     CDS             242..898
                     /locus_tag="ACHK3A_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO8743216.1"
                     /translation="MRTLGERLSSARQRAGLTQDALAKKAGITRVAISKAEQGLTKSF
                     NGDTLFKVATALNCSPQWLQNGEEQSVNWENNVKSCPQKDAFHSYPVINWVQAGLFST
                     AGDDYSMYDHDNWRHSVKYAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKAVHS
                     GCYVIARKKSTNEATFKKYVTEMGRSFLKPLNPQYPIIEIDDDCEIVGVIVDARWDIF
                     "
BASE COUNT          304 a          201 c          193 g          244 t
ORIGIN      
        1 tcttccggca cacctcgggt tttccacttt ccaacgccct gactacctct aggttttcct
       61 ctcttaggga aacggcgacc aattgcagca ttggttttaa actcagcttt taaaatttcg
      121 taaagagtca tctctcactc actgattgat actttagcat ccataaatgt acaacaaatg
      181 aatctaaagt atcaagaata tgtgctactt tggtatcgac cgaaaaaaca ggagagataa
      241 aatgagaact ttgggagagc gccttagcag cgccaggcaa cgggcaggat tgacacaaga
      301 tgcattagca aaaaaagccg gtataacccg cgtggctata agcaaagcag agcaaggact
      361 tactaaaagc ttcaacggag atactttatt caaggtagcc acagccctaa actgctctcc
      421 acaatggcta caaaatggag aagaacaatc tgtaaactgg gaaaacaatg ttaagagttg
      481 tcctcaaaaa gacgcatttc attcctatcc cgttattaac tgggtccagg ccggactatt
      541 ctctactgct ggagatgatt acagcatgta tgaccatgat aactggagac attctgtcaa
      601 atatgcaggt gaaagaggtt tttggcttga agtacatggt gattcaatga cctcgcctgt
      661 tggaatcacc tttcctgagg gtatgtctat tctcgtaaac ccagacaaag ctgtacattc
      721 aggctgttac gttatcgcaa gaaaaaaatc aaccaacgaa gcaacattca aaaaatatgt
      781 gactgaaatg ggaagatctt tcctgaaacc actcaatcct cagtatccca tcattgaaat
      841 agatgatgat tgtgaaatcg tcggcgtgat cgtggatgcc cgatgggata ttttctaaga
      901 aagatacttt ggtatcaaaa aacgcttgcc catgaatgat ac
//
LOCUS       DAZGHF010000401          924 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGHF010000401 DAZGHF010000000
VERSION     DAZGHF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.405"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..373)
                     /locus_tag="ACHK3A_005296"
     CDS             complement(<1..373)
                     /locus_tag="ACHK3A_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO8743217.1"
                     /translation="MARLLTIVLFFSISANAVTVNQFQRELDLADKTILWALISASTQ
                     EGRKACSLSYFSCKAAEAELGLAYMAANDNKAFLTSLSRIMMYKIDAGLSESYTCYLL
                     SKGKIIRPYLKNLNPHQLVADC"
     gene            complement(742..>924)
                     /locus_tag="ACHK3A_005297"
     CDS             complement(742..>924)
                     /locus_tag="ACHK3A_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743218.1"
                     /translation="KNVRIYIIDGKDRYYIDKNGVVSYGVQDNVICSNNKINEDSIEK
                     ILLEGNIDFWKKLKIK"
BASE COUNT          260 a          186 c          147 g          331 t
ORIGIN      
        1 tgcaatcagc gacaagttgg tgtggattta gattcttcag atatggtctt atgatctttc
       61 ccttgcttaa taaatagcat gtataggatt cggaaagtcc agcatctatt ttgtacatca
      121 ttattctaga taaggatgtg agaaatgcct tgttatcatt agcagccata tatgccagtc
      181 cgagttcagc ctctgcggct ttacatgaaa aatagctaag tgaacacgct tttcgtccct
      241 cttgggtact ggctgatatt agtgcccata atattgtctt gtctgccaaa tccaactctc
      301 tctgaaattg gtttacagta actgcattcg cagaaataga gaaaaaaagc acaattgtta
      361 ataaccttgc catatttcta gtccgtcact gttaatgtgc tatctggctc tgaaaatatt
      421 agctcattta atttttgcat gtcattagat gaaccagtta tacaaccctc ggaacagaat
      481 cgaggtccta aagatggacc aaaagcgttt aaacaactat gtgttaaaaa tccatcacga
      541 ttatacgttt ctgtatttgc tgacggtact aaacgtatac cattaggttt tctattggtt
      601 gctccaggac tgttaagcaa gcctcgtccg gtttgcttca ttgagttctc cactttagac
      661 tattggacta tctttcaaca tggcatcgcc ttagtatacc gctggttttc cgctcattgt
      721 taattttctc tttcgttcat ttcacttaat tttcagtttt ttccaaaagt caatattccc
      781 ttctaataaa attttttcta tggaatcttc attaatttta ttattagagc atatgacatt
      841 atcttgaacg ccatatgaca ccaccccgtt tttatcaata taatatctgt ctttaccatc
      901 aataatataa atcctaacat tttt
//
LOCUS       DAZGHF010000402          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGHF010000402 DAZGHF010000000
VERSION     DAZGHF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.406"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(72..398)
                     /gene="ybfB"
                     /locus_tag="ACHK3A_005298"
     CDS             complement(72..398)
                     /gene="ybfB"
                     /locus_tag="ACHK3A_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO8743219.1"
                     /translation="MKCIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(398..>916)
                     /locus_tag="ACHK3A_005299"
     CDS             complement(398..>916)
                     /locus_tag="ACHK3A_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8743220.1"
                     /translation="KGGWNFYQYPLNPITNIDPLGLETLKCIKPLHSMGGTGERSGPD
                     IWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVET
                     DNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNI
                     GRFIRFVFTGVM"
BASE COUNT          297 a          207 c          136 g          276 t
ORIGIN      
        1 cctgccgggt ctgtggcata cgggatatca atgcccaggt tacatataat aacaagagca
       61 attaacttaa tttatttgtt tataatgcgc ttacttaata caaaccattg aatataccct
      121 cctgacagaa aataaagcca tattattatc gccataatat acttactatc aatccttacg
      181 ccaataatgt ctgaaacagt ccctataaat attagagcaa taaagaatac tatgcctgtt
      241 ggaaaacata ttaccatcat tccataagac atgagactaa ttaactcgct cacatcacga
      301 gtggagtcta atagtgaaag tctatgcatg gaaaagaata gtattaaaag cgataaagcc
      361 agccatatag ctctgaataa aaaaataata catttcatta catcactccg gtaaatacaa
      421 atctaataaa acgtccaatg ttatttccgg agcacttcat cttacatgtt tctaaagaat
      481 cattagacca gtcttggcag tttttaaata gccctaaatt tataggtgtg aatatatcag
      541 gcaatacaga ataacgcggc cttacctctt taaacttccc ttttaaacag ttctccacac
      601 aactattatc ggtttcaacg aggtcacaac ggccagcagc ttcttttgta tcattacttg
      661 ctttacctgg cccccataga ccatctcctt ttgattctcc ccgttggtct tggccaccac
      721 aagtatagtc ccctttacca tctgggacac aaagatattg atgatagaac ggattccccc
      781 atatatctgg accgcttctt tcaccagttc cgcccattga atgcagtggc ttaatgcatt
      841 ttagtgtttc taatcctaaa ggatctatat tagtgatcgg attcaacgga tactgataaa
      901 aattccatcc cccctt
//
LOCUS       DAZGHF010000403          912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGHF010000403 DAZGHF010000000
VERSION     DAZGHF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.407"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..748
                     /gene="ompT"
                     /locus_tag="ACHK3A_005300"
     CDS             <1..748
                     /gene="ompT"
                     /locus_tag="ACHK3A_005300"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO8743221.1"
                     /translation="AIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPG
                     TWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYS
                     SEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDN
                     DEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYD
                     HNNNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          307 a          190 c          191 g          224 t
ORIGIN      
        1 tgcaattatt aaaggtgcaa ttaattggga tttgatgccc cagatatcta tcggggctgc
       61 tggctggaca actctcggca gccgaggtgg caatatggtc gatcaggact ggatggattc
      121 cagtaacccc ggaacctgga cggatgaaag tagacaccct gatacacaac tcaattatgc
      181 caacgaattt gatctgaata tcaaaggctg gctcctcaac gaacccaatt accgcctggg
      241 actcatggcc ggatatcagg aaagccgtta tagctttaca gccagaggtg gttcctatat
      301 ctacagttct gaggagggat tcagagatga tatcggctcc ttcccgaatg gagaaagggc
      361 aatcggctac aaacaacgtt ttaaaatgcc ctacattggc ttgactggaa gttatcgtta
      421 tgaagatttt gaactcggtg gcacatttaa atacagcggc tgggtggaat catctgataa
      481 cgatgaacac tatgacccgg gaaaaagaat cacttatcgc agtaaggtca aagaccaaaa
      541 ttactattct gttgcagtca atgcaggtta ttacgtcaca cctaacgcaa aagtttatgt
      601 tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat acttcacttt atgatcacaa
      661 taataacact tcagactaca gcaaaaatgg agcaggtata gaaaactata acttcatcac
      721 tactgctggt cttaagtaca cattttaaga acgccaacta aaatttcccc gaggtgaaaa
      781 tcgccccggg gaataactag ccatttcaat gtaacaatta acccttaaaa taaacccaga
      841 aggttattaa ctaaatcaca tagaaaacca tcaattatag tatgtataaa ataggcgaca
      901 gcaacccaat ta
//
LOCUS       DAZGHF010000404          908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGHF010000404 DAZGHF010000000
VERSION     DAZGHF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.408"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..183
                     /locus_tag="ACHK3A_005301"
     CDS             <1..183
                     /locus_tag="ACHK3A_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO8743222.1"
                     /translation="DGIVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVK
                     TKVQGTVVMFSASGKK"
     gene            549..662
                     /locus_tag="ACHK3A_005302"
     CDS             549..662
                     /locus_tag="ACHK3A_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO8743223.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            731..>908
                     /gene="ydfK"
                     /locus_tag="ACHK3A_005303"
     CDS             731..>908
                     /gene="ydfK"
                     /locus_tag="ACHK3A_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO8743224.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKEL"
BASE COUNT          313 a          189 c          149 g          257 t
ORIGIN      
        1 gatggaattg tgcacaatag cttcggtcaa aaactgatgc gcatttacaa tcagaaaggc
       61 atcttttcaa acacaaaaga ttccgaagaa ggcttaacac atatactctc agagcatttc
      121 gagaatgtta aaaccaaggt tcaaggtact gtagtaatgt tttccgcttc agggaaaaaa
      181 tagcatccaa ccgcagcacg ttcttgcata aggcgtgtct gcggtttttc aactattcag
      241 atacatcact cccatcacat tcattcctcc gcatcatagg catataggct atatcacctt
      301 gatatttttc ttcttcagat aaaaactgtt atctatgtat acttttaaac ccaatccgtg
      361 tagagtctct gcataagata gtttgcagtt gacacttcag cttgcgccat aaaccgcctg
      421 atttttgccg ccacctgtta gcattcctgt atacctgaaa cgacaatgtt tatctacgaa
      481 ctttaagaac acccaggata aaaattgtca actatatcat atataacaca ttactaattc
      541 gaggctatat gaacagcata ctgataatca cttcgctcct tatcatattc agcattttta
      601 gtcatgctct aataaaatta gggattggca tatccaataa cccagacaaa accgatgtat
      661 aagtcaacat atcctgaatc agacatacaa tatcgcaatg aaaatcaata atattttaag
      721 gaatatcttc atgaaatcaa aagacaccct aaagtggttc cctgcgcagc ttcctgaagt
      781 aagaattatc ctaggggatg ctgtagtgga agtagcaaaa cagggaagac ctatcaatac
      841 cagaacattg cttgattaca ttgaaggaaa cataaagaaa aagtcatggc tggataacaa
      901 agaattat
//
LOCUS       DAZGHF010000405          906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGHF010000405 DAZGHF010000000
VERSION     DAZGHF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.409"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..782
                     /locus_tag="ACHK3A_005304"
     CDS             <1..782
                     /locus_tag="ACHK3A_005304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743225.1"
                     /translation="RNMEKSFIREKLRPNTTELTGVVFIACCASLHAENSLMPGWKNT
                     TPGLISIAGLSRKDHLRREQTMTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFD
                     EPIWHGLEIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNE
                     NGGTDKGTMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTG
                     ALGDGEKTGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            818..906
                     /locus_tag="ACHK3A_005305"
                     /pseudo
     CDS             818..906
                     /locus_tag="ACHK3A_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
BASE COUNT          266 a          208 c          231 g          201 t
ORIGIN      
        1 tccggaatat ggagaagtct ttcatccggg agaaattacg cccgaatact acagagctca
       61 cgggcgttgt tttcatcgcc tgttgcgcat ccttgcacgc agaaaacagt ttgatgcctg
      121 gatggaaaaa tacaacgcct ggattgatca gtattgccgg cttgtcgcgc aaagaccatt
      181 taagaagaga gcaaaccatg actgcaactg aacagttatt ttttcttacc gctgaaggta
      241 tccacacacc ttacggactg cttgatatca acaaagcact ggaaatgctc gatgaaggct
      301 gttttgacga gccgatctgg catggtctgg aaatagtgtg ttgcatcagt gaagcagaag
      361 aaaaaggcat gacaggtgag ctcgcgccgg aaaagacaat catgtactgg cgctggcttt
      421 ataccgccat gttcttctct gaacagctca ccagaaacgg ctggaccgaa gtgcttaacg
      481 agaatggcgg tacagacaag ggcaccatgt acagaggcca caacggtggc atgacaatct
      541 acctgaaacc attacgtctc gctatgcaga actatatgga atgggcactc atagaaactt
      601 acggagcgga atcagggctg ctcaaaagca ttcttatgta caaagcattc accggcgctc
      661 tgggcgacgg cgaaaaaacc ggcatcacaa tggaaggtta cagagcgatg cacatgattc
      721 tggatgggct tatcagtgaa gccactgaca actcagaacc ggaaaccaga gtgttccact
      781 aatcagcttt tcgtgaggtg taatgatgaa aaaatcaact gaacttgtgc cggtggcagc
      841 ccgtgatttg caaattatcg aatatcgcgg tcaacgcgtt gtgaccactg aacaactggc
      901 ggcagg
//
LOCUS       DAZGHF010000406          896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGHF010000406 DAZGHF010000000
VERSION     DAZGHF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.410"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            56..817
                     /gene="ydeO"
                     /locus_tag="ACHK3A_005306"
     CDS             56..817
                     /gene="ydeO"
                     /locus_tag="ACHK3A_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO8743226.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTDNTMSALAI"
BASE COUNT          279 a          170 c          177 g          270 t
ORIGIN      
        1 aatatgaaat gttaaaaaag tatcgataaa aactttattg ttttaaggag ataaaatgtc
       61 gctcgtttgt tctgttatat ttattcatca tgccttcaac gctaacattt tagataaaga
      121 ttacgccttc tctgacggcg agatcctgat ggtagataac gctgttcgta cgcattttga
      181 accttatgag cggcatttca aagaaatcgg atttaatgaa aataccatta aaaaatatct
      241 acaatgcact aacatccaga cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc
      301 aaatgtaccg actggattgc ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa
      361 tcatcataat ttttcagaac ttttgctttt ttcttgcctg tctatttttg ccacatgcaa
      421 aggtttcatt acactattaa ctaacggtgt gctatccgtt tccgggaaag tgagaaatat
      481 tgtcaacatg aagctggcgc acccatggaa gctgaaagat atttgtgact gcctgtacat
      541 tagtgaaagc ctgttgaaga aaaaactaaa gcaagagcaa acgacattct cacagattct
      601 tttagatgcc agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa
      661 aattgccgaa caatgtggtt atgcgagtac atcttatttt atttatgcgt tccgcaaaca
      721 tttcggcaac agtccgaaga gagtttctaa ggagtaccgt tgtcaaaggc acacgggtat
      781 gaacacggat aacacgatga gtgctttagc tatttgatta tttgctaacg agtagtcaac
      841 cacgcacgtt acgtagaaat taaaatgggg cagccattcc ctgccccgcg ttgttt
//
LOCUS       DAZGHF010000407          889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGHF010000407 DAZGHF010000000
VERSION     DAZGHF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.411"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            45..845
                     /locus_tag="ACHK3A_005307"
     CDS             45..845
                     /locus_tag="ACHK3A_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743227.1"
                     /translation="MNSKKVCCICVLFSLLAGCASQSSIDEKKKKAQVTQSNINKNIP
                     PQLTDKDLFGNETTLAVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YHDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKMSNKELSNDHVTEVQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          279 a          167 c          207 g          236 t
ORIGIN      
        1 gctaaatatt ctaatcatta tgacaggcga gggagtgtcc gattatgaat tcaaaaaagg
       61 tttgttgcat atgtgtatta ttctcgctgc ttgcaggatg tgcctctcaa tcttctattg
      121 atgaaaaaaa gaaaaaagct caagttacac aaagtaatat taataaaaac attcccccgc
      181 aactgacaga caaagattta ttcggtaatg aaaccactct ggctgtatcc gaggaggata
      241 ttcaggctgc gcttgaagga gatgagtttc gtgtgcctct caattctccc gtaatacttg
      301 tccagtctgg cagtcgcgca ccggaaacca ttatgcagga ggagatgaga aaatattata
      361 ctgtttccac attctctggt atcccggaca ggcaaaagcc tctgacttgt aacaaaaata
      421 aagataaaaa tgagaacgag gatgttgcca gtgctgaaaa tatgaactgg atgcaggcat
      481 tgcgttttgt ggctgcgaaa gggcatcaga aagcgattat tgtttaccat gatacgttgc
      541 agacaggaaa atatgactct gcgctgaaat caacagtatg gtccgactat aaaaatgaaa
      601 aactcactga cgctatctcc ctgcgctacc tggtacgttt cacgctggtg gatgtggcaa
      661 caggtgagtg ggctacctgg tcgccggtga attatgaatc gagagtaatt tttccgccgg
      721 ctggaataac aaaaatgagc aataaagaac tatcaaatga ccatgttacg gaagtgcaac
      781 tgtttaattt gaaacaaaaa acatatgtat cgatggtgaa agacttggtg agtcgattcc
      841 agtaatcgtt attgttatgc tgccgcctgt agtttagggc gggcagcaa
//
LOCUS       DAZGHF010000408          887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGHF010000408 DAZGHF010000000
VERSION     DAZGHF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.412"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            258..473
                     /gene="essD"
                     /locus_tag="ACHK3A_005308"
     CDS             258..473
                     /gene="essD"
                     /locus_tag="ACHK3A_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO8743228.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            473..>887
                     /gene="rrrD"
                     /locus_tag="ACHK3A_005309"
     CDS             473..>887
                     /gene="rrrD"
                     /locus_tag="ACHK3A_005309"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO8743229.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRW"
BASE COUNT          236 a          198 c          210 g          243 t
ORIGIN      
        1 caatactcgg tagcattctc gcaaatatca ttttgtggtt tacgtacgta aaaaattggt
       61 tatgctgtta agagtggtta cttcgtcaca cagcttaaac ccgccgtcga gcgggttttt
      121 ccattttttg agtctcgata ttagctgata acccaatacc tgagttattc actgactccg
      181 agtctgttac gtttctgctt ttttgcgata cgttgtattc cctcaattta cacccgcttt
      241 gtctgcgagg tggggttatg aaatccatgg ataagttaac aacgggtgtc gcctatggca
      301 cctcagcagg tagtgccggt tactggtttt tacagctgct cgataaagtc acgccctcac
      361 agtgggcagc aataggtgtg ctgggtagcc tggtatttgg cctgctgacg tacctgacaa
      421 acctttattt caagattaaa gaagataagc gcaaggctgc gagaggtgaa taatgcctcc
      481 atcattacga aaagccgttg ctgctgctat tggtggcgga gcaattgcta tagcatcagt
      541 gttaatcact ggcccaagtg gtaacgatgg tctggaaggt gtcagctaca taccatacaa
      601 agatattgtt ggtgtatgga ctgtatgtca cgggcataca ggaaaagaca tcatgctcgg
      661 taaaacgtat accaaagcag aatgcaaagc cctcctgaat aaagaccttg ccacggtcgc
      721 cagacaaatt aacccgtaca tcaaagtcga tataccggaa acaatgcgcg gcgctcttta
      781 ctcattcgtt tacaacgtgg gtgctggcaa tttcagaaca tcgacgcttc ttcgcaaaat
      841 aaaccagggc gatatcaaag gcgcatgtga tcagctacgt cgctgga
//
LOCUS       DAZGHF010000409          884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGHF010000409 DAZGHF010000000
VERSION     DAZGHF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.413"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..755)
                     /locus_tag="ACHK3A_005310"
     CDS             complement(<1..755)
                     /locus_tag="ACHK3A_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743230.1"
                     /translation="MNPTKRLLLGNDTIQLVSCSVMLELNACGRGFITARTEQNYTGR
                     PVRLDIGYGNDLVRWFTGYVERSQPAENGSVRLLIREMAGILDHPFPCSFQHPTMRTV
                     TQWLSETSGLEIILPDNAAYTDRPVPHFTHSGTGYELLASLGRIFSVPDYIWHPLPDG
                     GIMAAAAAQGMFSGRPVTIPHEFSQASTGGHSMTLPMIQTLRPGVEVNGQRLTMVMLE
                     DDNMTITWTPRNKATGAPLQKSPFRRQAEKAFP"
BASE COUNT          163 a          245 c          273 g          203 t
ORIGIN      
        1 tcagggaagg ccttttcagc ctggcgacgg aaaggggact tctggagggg agcgccggta
       61 gctttattgc gcggtgtcca cgtgatggtc atgttgtcat cttccagcat gaccatcgtc
      121 aggcgctgac cgttcacctc cacacccggt cgcagcgtct gaatcatcgg gagggtcata
      181 ctgtgcccgc ccgttgatgc ctggctgaac tcgtgcggga tggtgacggg gcgaccggaa
      241 aacatcccct gcgcggcagc agcggccata atgccgccgt ccggtaacgg atgccagatg
      301 taatccggta cggagaaaat acgccccaga ctggccagca gttcgtagcc cgtgccgctg
      361 tgtgtgaaat gcggaacggg tctgtcagtg taagccgcgt tgtccggcag gatgatttcc
      421 agcccgctgg tctcactcag ccactgtgtg accgtgcgca ttgtcgggtg ctgaaatgaa
      481 caggggaagg ggtgatcaag gatccccgcc atttcacgaa tcagaagccg caccgagccg
      541 ttttcagcgg gctggctgcg ttcaacgtat ccggtaaacc agcgcaccag gtcgtttccg
      601 tatccgatat cgagacgaac agggcgcccg gtgtagttct gctccgttct ggccgtgata
      661 aatccgcgtc cgcacgcgtt cagctcaagc atgacggaac agctgaccag ctgaatggtg
      721 tcgttaccca gtaacagacg tttagtgggg ttcatggtta ctccagcgca tcattgacgg
      781 gtttgaggac cttttcttcg aaccaggtca ttttctccgg tgtttcgttg gccacggttt
      841 tgccttttgc gtcgggcgtc tgcggcgtct gtttacggct gctg
//
LOCUS       DAZGHF010000410          884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGHF010000410 DAZGHF010000000
VERSION     DAZGHF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.414"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..755)
                     /locus_tag="ACHK3A_005311"
                     /pseudo
     CDS             complement(1..755)
                     /locus_tag="ACHK3A_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          162 a          245 c          275 g          202 t
ORIGIN      
        1 tcagggaagg ccttttcagc ctggcgacgg aaaggggact tctggagggg ggcgccggtg
       61 gctttattgc gtggtgtcca cgtgatggtc atgttgtcat cttccagcat gaccatcgtc
      121 aggcgctgac cgttcacctc cacacccggt cgcagcgtct gaatcatcgg gagggtcata
      181 ctgtgcccgc ccgttgatgc ctggctgaac tcgtgcggga tggtgacggg gcgaccggaa
      241 aacatcccct gcgcggcagc agcggtcata atgccgccgt ccggtaacgg atgccagatg
      301 taatccggta cggagaaaat acgccccaga ctggccagca gttcgtagcc cgtgccgctg
      361 tgtgtgaaat gcggaacggg tctgtcagtg taagccgcgt tgtccggcag gatgatttcc
      421 agcccgctgg tctaactcag ccactgtgtg accgtgcgca ttgtcgggtg ctgaaatgaa
      481 caggggaagg ggtgatcaag gatccccgcc atttcacgaa tcagaagccg caccgagccg
      541 ttttcagcgg gctggctgcg ttcaacgtat ccggtaaacc agcgcaccag gtcgtttccg
      601 tatccgatat cgagacgaac ggggcgcccg gtgtagttct gctctgttct ggctgtgata
      661 aatccgcgtc cgcacgcgtt cagctcaagc atgacggaac aactgaccag ctgaatggtg
      721 tcgttaccca gtaacagacg tttagtgggg ttcatggtta ctccagcgca tcattgacgg
      781 gtttgaggac cttttcttcg aaccaggtca ttttctccgg tgtttcgtcg gccacggtcc
      841 cgccttttgc gccgggcgtc tgcggcgtct gtttacggct gctg
//
LOCUS       DAZGHF010000411          883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGHF010000411 DAZGHF010000000
VERSION     DAZGHF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.415"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>883
                     /locus_tag="ACHK3A_005312"
     rRNA            <1..>883
                     /locus_tag="ACHK3A_005312"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          206 a          201 c          280 g          196 t
ORIGIN      
        1 ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
       61 gttccgacct gcacgaatgg cgtaatgatg gccaggctgt ctccacccga gactcagtga
      121 aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc
      181 tttactatag cttgacactg aacattgagc cttgatgtgt aggataggtg ggaggctttg
      241 aagtgtggac gccagtctgc atggagccga ccttgaaata ccacccttta atgtttgatg
      301 ttctaacgtt ggcccctgat cggggttgcg gacagtgtct ggtgggtagt ttgactgggg
      361 cggtctcctc ctaaagagta acggaggagc acgaaggttg gctaatcctg gtcggacatc
      421 aggaggttag tgcaatggca taagccagct tgactgcgag cgtgacggcg cgagcaggtg
      481 cgaaagcagg tcatagtgat ccggtggttc tgaatggaag ggccatcgct caacggataa
      541 aaggtactcc ggggataaca ggctgatacc gcccaagagt tcatatcgac ggcggtgttt
      601 ggcacctcga tgtcggctca tcacatcctg gggctgaagt aggtcccaag ggtatggctg
      661 ttcgccattt aaagtggtac gcgagctggg tttagaacgt cgtgagacag ttcggtccct
      721 atctgccgtg ggcgctggag aactgagggg ggctgctcct agtacgagag gaccggagtg
      781 gacgcatcac tggtgttcgg gttgtcatgc caatggcact gcccggtagc taaatgcgga
      841 agagataagt gctgaaagca tctaagcacg aaacttgccc cga
//
LOCUS       DAZGHF010000412          880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGHF010000412 DAZGHF010000000
VERSION     DAZGHF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.416"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>880
                     /locus_tag="ACHK3A_005313"
     CDS             <1..>880
                     /locus_tag="ACHK3A_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFO8743231.1"
                     /translation="SEDAVSEHLRFRYGRRWQHKVRLLLVMGDPDVVRKAAPGLCHDL
                     WQEGDGYVLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALPTDGERDALL
                     RYRQKADHQLGWQAPVWLWLTDNATGARQDAKAAATGVIFGPEGTVKGADEAFSTLAL
                     HLQKAGMAQILDEPADFGLLQLSLRLRQELKASLAVLLSGLMQGTAAWRLRGVMFSPE
                     LTVAGAVPNTCPDSPAWKAVIDDCGAASGRKLGFSWLKMLRLILLSLIVLWGTGTLLS
                     LLVNRTQIYQAQETAGQ"
BASE COUNT          177 a          237 c          278 g          188 t
ORIGIN      
        1 agcgaagatg ctgtttcaga acatttgcgc tttcgctatg ggcgtcgctg gcagcataag
       61 gtgcgactcc tgctggtgat gggggatccg gatgtggtcc ggaaagcagc cccgggcctt
      121 tgccatgacc tctggcagga aggcgacggc tacgtgctga tttatggcgg agacgcgcag
      181 tcactcccgg acgagatctt cctttcaaaa ctgaagcgtc tgcgatccgg gcaaccggtt
      241 gatggcatca ttcaggtgat gagcatttca gccctcccga cggatggcga acgggatgcc
      301 ctcctgcgtt accgccagaa agccgaccat cagttgggct ggcaggcacc agtctggctg
      361 tggctgactg acaacgctac cggtgcccgg caggacgcga aagcggccgc aacaggggtc
      421 atcttcggac cggaaggaac tgtgaaaggt gcggacgaag cgttttccac actggcgtta
      481 cacctgcaaa aagcgggaat ggcgcagata ctggatgagc ctgctgattt cggtctcctt
      541 cagttgtcac tcagactgcg gcaggagctg aaagccagcc tggctgtttt gctcagtgga
      601 ctgatgcagg ggactgctgc ctggcgttta cgcggtgtca tgttcagtcc tgaactgacc
      661 gttgccggag cggtaccaaa cacatgcccc gactcaccgg cctggaaagc cgtcattgat
      721 gactgtggtg ccgccagcgg cagaaaactg ggcttcagct ggctgaagat gctgcgattg
      781 attctgcttt ccctgattgt cctgtggggg accggtacac tgctgtctct gctcgttaac
      841 cgtactcaga tttatcaggc gcaggaaacg gcgggacagg
//
LOCUS       DAZGHF010000413          877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGHF010000413 DAZGHF010000000
VERSION     DAZGHF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.417"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>877
                     /locus_tag="ACHK3A_005314"
     CDS             <1..>877
                     /locus_tag="ACHK3A_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO8743232.1"
                     /translation="VPNNEAIVSVAQKSFGKEMAMIMFFAMVINIMIARFTPWKFIFL
                     TGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAMVFFPAIAHPYMKKVTGSDDV
                     AIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLFLRDTPVAISFTMSIIFLVTC
                     LFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQGVRMVIAEIVPAFKGISDKLV
                     PNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTLYLLNMIVIIPGVVPHFFVGA
                     AAGVFGNATGGRRGA"
BASE COUNT          182 a          194 c          235 g          266 t
ORIGIN      
        1 tagtgccgaa caatgaagcc attgtttccg tagcgcagaa aagcttcggg aaagaaatgg
       61 cgatgattat gttctttgcg atggttatca acattatgat tgcccgtttc acaccgtgga
      121 aatttatctt cctgaccggt catcacacgt tgtttatgtc gatgatggta gcggtaattc
      181 tggcaacagc aggcatgacc ggcattacgc ttatagccgt tggctctttg gttgttggcg
      241 tggcaatggt ctttttcccg gctattgcgc atccgtacat gaagaaagtg accggttctg
      301 atgacgttgc catcggccac ttctcaacat tatcttatgt attggccggt ttcatcggca
      361 gcaagtttgg taataaagag cactcgactg aagacatgaa tgtgccgaaa agtctgctgt
      421 tcttgcgcga cacgccggtt gctatctctt ttaccatgag cataattttc ctggtgacgt
      481 gcctgtttgc gggtgcagat gcggtgaaag agcttagcgg tggtaaaaac tggttcatgt
      541 tctctatcat gcaatccatt accttcgcag ctggcgtgta catcattctg cagggtgtgc
      601 gcatggtgat tgcggagatt gtcccggcat tcaaaggtat ttcagacaag ctggttccga
      661 atgccagacc tgctctcgat tgcccggtcg tcttccctta cgcacctaac gctgtactgg
      721 ttggtttcct gagtagcttt gctgcaggtt taatcggtat gttcacgttg tatctgctga
      781 acatgatcgt gattattcct ggtgtggtgc cgcacttctt cgtaggtgca gctgcagggg
      841 tattcggtaa tgcaacgggt ggacgtcgtg gtgcgat
//
LOCUS       DAZGHF010000414          876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGHF010000414 DAZGHF010000000
VERSION     DAZGHF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.418"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>876
                     /locus_tag="ACHK3A_005315"
     CDS             <1..>876
                     /locus_tag="ACHK3A_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO8743233.1"
                     /translation="AVPGCTIELKARRTTETVIVTTVAYGQPGETGSYSMDVEPGLYR
                     VTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAVAAEILKRA
                     EASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGETGPQGLQG
                     VKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAATPKAVKAAMDKADGCL
                     EKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEFVAWLKEKGAFDQPYWMM
                     KASLPAVLNKVITDVG"
BASE COUNT          241 a          187 c          323 g          125 t
ORIGIN      
        1 agcggtaccg ggatgcacga tagagctgaa agcgcgccgc acaacggaga cggtgattgt
       61 caccacggtg gcgtatggtc agccggggga aaccggcagt tacagtatgg atgttgagcc
      121 ggggttgtac cgggtgacgc tgaacacgga agggtacgcg ccgtcatatg tgggtgacat
      181 tctggtgaag gcggattctg caccgggaac gctgaataaa tttctgatgg acctggagga
      241 cgcacagtat tacccgaaag cccttgcaga gctggaagcg gtggcagcgg aaatcctgaa
      301 acgtgcagaa gcgtcagcgg ccagtgcaga ggaagcgaag aaacgggcag agaatgcgcg
      361 gggaccgaag ggggataagg gagacaccgg gccgcagggt attcccgggc caaaaggcga
      421 taccggcgag cgggggccaa agggtgagcg tggtgagaca ggaccacagg ggcttcaggg
      481 tgtgaaaggt gaacggggag agaagggcga aaaaggcgag ccgggaggac cggatgcgac
      541 gacggcacag aagggaattg tgcagttaag cagcgcaacg gacagtgatg atgaaacgaa
      601 ggcagccacc ccgaaagcgg tgaaagcggc aatggacaaa gcggacggat gcctggagaa
      661 agcgaaaaac ggtgacgata tcccggataa ggtgaagttt ctgaacaccg tgggagcagc
      721 cagagtatac gggcgggaca ttcatacggg ggccggtgaa tggaccacga gcgagtttgt
      781 cgcctggctg aaagaaaaag gggcatttga ccagccttac tggatgatga aggcatcact
      841 gcctgcggta ctcaataaag tcatcacgga tgtcgg
//
LOCUS       DAZGHF010000415          860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGHF010000415 DAZGHF010000000
VERSION     DAZGHF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.419"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..402
                     /locus_tag="ACHK3A_005316"
                     /pseudo
     CDS             1..402
                     /locus_tag="ACHK3A_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            394..729
                     /locus_tag="ACHK3A_005317"
     CDS             394..729
                     /locus_tag="ACHK3A_005317"
                     /inference="COORDINATES: protein motif:HMM:NF026979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain fold toxin domain-containing protein"
                     /protein_id="HFO8743234.1"
                     /translation="MAKTRDLATTISDKFKQLFQCDKFAAALKSKMQQAGVNGEHLIL
                     ETKNKQGAFGNIWSDRIGKNIATNGHHEAIKVGDTVFDNMNPSGIKYEDWLNDIYSPG
                     EYTITPSRF"
BASE COUNT          265 a          172 c          219 g          204 t
ORIGIN      
        1 ccgtcggtgg ccccgcagtg ggtgacgcgg acgaatcatg cggtgagcga cctgacgggc
       61 cgtccgctga tgctctttaa cagtgaaggt aaaaccgtgt ggcgaccgtg acaaaccagc
      121 ctgtgggggc tggcactcag tctgcccgca gacaccggct acccggaccc gcgcggggag
      181 ctggacccgg aagccgaccc cggcctgctg tatgcaggac agtggcagga tacggaatcc
      241 gggctgtgct ataaccggtt ccggtattac gagccggaaa cagggatgta cctggtgagt
      301 gacccgatag ggctgagagg cgggataaac aattatgcat atgttcataa tccccttggt
      361 tatattgatc cattaggatt aaccatttgc gatatcgcaa aaacaaggga tcttgcaaca
      421 acaataagtg ataagtttaa acagcttttt caatgcgata aatttgccgc agcgttaaaa
      481 agtaaaatgc aacaagctgg ggtaaatggt gagcatctca tactcgaaac taaaaataaa
      541 caaggtgcct ttggcaatat atggagtgat agaataggta aaaatatagc aactaatggt
      601 catcatgaag caataaaagt aggtgataca gtttttgata atatgaatcc ttctggtata
      661 aagtatgaag attggctgaa tgatatatac tctcctggcg aatacaccat tactccatca
      721 aggttttaat tatgaatgat attgttaaac tgattgatat gatagaaaaa agaccagctt
      781 tatatttaag caggcatacc atatcggcgt taaaagcttt cctggatgga tggcaaatga
      841 gagagccaaa agaaatcgtc
//
LOCUS       DAZGHF010000416          859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGHF010000416 DAZGHF010000000
VERSION     DAZGHF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.420"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..735
                     /locus_tag="ACHK3A_005318"
     CDS             <1..735
                     /locus_tag="ACHK3A_005318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743235.1"
                     /translation="TTELTGVVFIACCASLHAENSLMPGWKNTTPGLISIAGLSRKDH
                     LRREQTMTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGLEIVCCISE
                     AEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGRTEVLNENGGTDKGTMYRGHNG
                     GMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEKTGITMEGY
                     RAMHMILDGLISEATDNSEPETRVFH"
     gene            771..859
                     /locus_tag="ACHK3A_005319"
                     /pseudo
     CDS             771..859
                     /locus_tag="ACHK3A_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
BASE COUNT          257 a          198 c          214 g          190 t
ORIGIN      
        1 actacagagc tcacgggcgt tgttttcatc gcctgttgcg catccttgca cgcagaaaac
       61 agtttgatgc ctggatggaa aaatacaacg cctggattga tcagtattgc cggcttgtcg
      121 cgcaaagacc atttaagaag agagcaaacc atgacagcaa ctgaacagtt attttttctt
      181 actgctgaag gtatccacac accttacgga ctgcttgata tcaacaaagc actggaaatg
      241 ctcgatgaag gctgttttga cgaaccgatc tggcatggtc tggaaatagt gtgttgcatc
      301 agtgaagcag aagaaaaagg catgacaggt gagctcgcgc cggaaaagac aatcatgtac
      361 tggcgctggc tttataccgc catgttcttc tctgaacagc tcaccagaaa cggccgtacc
      421 gaagtgctta acgagaatgg cggtacagac aagggcacca tgtacagagg ccacaacggt
      481 ggcatgacaa tctacctgaa accattacgt ctcgctatgc agaactatat ggaatgggca
      541 ctcatagaaa cttacggagc ggaatcaggg ctgctcaaaa gcattcttat gtacaaagca
      601 ttcaccggcg ctctgggcga cggcgaaaaa accggcatca caatggaagg ttacagagcg
      661 atgcacatga ttctagatgg gcttatcagt gaagccactg acaactcaga accggaaacc
      721 agagtgttcc actaatcagc ttttcgtgag gtgtaatgat gaaaaaatca actgaacttg
      781 tgccggtggc agcccgtgat ttgcaaatta tcgaatatcg cggtcaacgc gttataacca
      841 ctgaacaact ggcggcagg
//
LOCUS       DAZGHF010000417          856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGHF010000417 DAZGHF010000000
VERSION     DAZGHF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.421"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..788
                     /locus_tag="ACHK3A_005320"
     CDS             <1..788
                     /locus_tag="ACHK3A_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO8743236.1"
                     /translation="NETAKQFNTSIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYL
                     SESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGR
                     YFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKD
                     FDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQS
                     PWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          284 a          159 c          153 g          260 t
ORIGIN      
        1 ttaatgaaac ggcaaaacaa tttaatacct caattattgt atatttaatt gaccccaaat
       61 actttgctga tctgccgacg tcacagtttt ggtcgtacgc aacatacttc agggtattgt
      121 cttttgaata tctgagtgaa agtatttcta cactgctgta tctggatgcc gatgttgttt
      181 gtaaaggaag cctgaaacct ctcacagaaa ttatatttaa agatgagttt gctgcggtca
      241 ttcctgacaa tgatagtact caggcggcat gtgcaaaacg cctcaacatt cccgaaatga
      301 atggacgtta tttcaatgca ggcgttatct atgtcaatct taaaaaatgg catgaagcaa
      361 atttgacacc gtatttactc acgcttttac gaggggaaac taaatatggc tctcttaaat
      421 atttagatca ggatgcgttg aatatcgcat ttaatatgaa taatatctac ctcgcgaagg
      481 attttgatac tatttatacc ctgaaaaacg aacttcatga tcgtagtcat cgaaagtatc
      541 agcaaaccat taccgataaa acagtattga ttcactatac agggataact aaaccatggc
      601 atagctgggc tggatatcca tctgcatcat actttaatat cgcgcgtgaa caatctccct
      661 ggaagaaata tcctcttaaa gaggcgcgga ctgttgcaga aatgcagaaa caatataagc
      721 atctgtttgc ccatggtgag tatattaaag gtataacttc attaattaag tacaagctta
      781 agaaataaat tactcttatg attacaagta tacgctatcg cggcttctca ttttattaca
      841 aagataacga taataa
//
LOCUS       DAZGHF010000418          854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGHF010000418 DAZGHF010000000
VERSION     DAZGHF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.422"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>854
                     /locus_tag="ACHK3A_005321"
     CDS             <1..>854
                     /locus_tag="ACHK3A_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO8743237.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGPDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNLHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDMQGHEMRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TQISHLSEG"
BASE COUNT          216 a          249 c          244 g          145 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacaccggat ggcagggcat cggcatttta ctataaccac
       61 cacagccagt taacgtcagc caccgggcct gacgggctgg aattgcgccg ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatctac acagtgactt accctgcgca acggaagatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacat gcagggccat
      421 gaaatgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtaccac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggcttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac acaaatcagc
      841 catctcagcg aagg
//
LOCUS       DAZGHF010000419          854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGHF010000419 DAZGHF010000000
VERSION     DAZGHF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.423"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>854
                     /locus_tag="ACHK3A_005322"
     CDS             <1..>854
                     /locus_tag="ACHK3A_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO8743238.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGTDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TGISHLSEG"
BASE COUNT          212 a          250 c          249 g          143 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacgccggat ggcagggcat cggcgtttta ctataaccac
       61 cacagccagt taacgtcagc caccgggact gacgggctgg agttgcgcag ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatccac acagtgactt accctgcgca acggacgatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacac gcagggccat
      421 gaaacgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtaccac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggtttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac aggcatcagc
      841 catctcagcg aagg
//
LOCUS       DAZGHF010000420          846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGHF010000420 DAZGHF010000000
VERSION     DAZGHF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.424"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            485..>846
                     /locus_tag="ACHK3A_005323"
     CDS             485..>846
                     /locus_tag="ACHK3A_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO8743239.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPG"
BASE COUNT          198 a          219 c          230 g          199 t
ORIGIN      
        1 gtatccgttc aacaggaatg ttacatacct cacaattaag tcagttgaat gttgtctgcc
       61 cggatgagaa tttgttagaa aaaaactgca tggtgaatcc ccctgtgcgg aggggcatat
      121 cagcgacagg tgtttggtta catcccttat cctttctgtg cgggttcagg tgctgatact
      181 gaactcaccg ggaggcaccc ggcaccatgc gcatgatgat acagatacgc agcgtcagcc
      241 cctctccgga ggggctttct tatggataaa aaagcccgca acaatgtgcg ggcaaacatg
      301 attaacactt tcaaaaagca atgaatttag cggacagatt ttaaccagaa ttcataaggc
      361 tgcgcaactg cgcggccttt ttcgtattgc gggctgtagt cttcctcctg tcattgtcct
      421 gtaacttccg gacttcagcc cgccccttat ctgactcaca acattatccc gaccgggagg
      481 attcatggca tttaaacact acgatgtggt cagggcggcg tcgccgtcag accttgcgga
      541 gcgactgact caaaaactga aggaggggtg gcagccattt ggcagtcctg tcgccatcac
      601 gccttatacc ctgatgcagg ccattgcggc ggaaggtgat gtcaccacac ctgtgttggt
      661 gaagccgtcg gatggagaag gcacagttat cagcgccacc agcgacccgg agtattactt
      721 tgttgtggtt ctggcagggc agtcaaacgg catgtcgtat ggtgaaggcc ttccgctgcc
      781 ggagacatat gaccgtccgg acccgcgtat taagcagctg gcgcgccgca gtacggtgac
      841 accggg
//
LOCUS       DAZGHF010000421          844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGHF010000421 DAZGHF010000000
VERSION     DAZGHF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.425"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>844
                     /locus_tag="ACHK3A_005324"
     CDS             <1..>844
                     /locus_tag="ACHK3A_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO8743240.1"
                     /translation="DDSRTSHALVKHNYQRLLDKIDSGSDRYSPMEYWRALHNPDYRD
                     VLQKTIVKHPSDWYFKKDDAIWQPFLNALKKDAPEWKKYSEDFLDKMAWMQDVTTEKL
                     GPSLWHMHPIMFLASLIQPNEDIRILRLRAFLRMIRIGEGTIQEDGYRTMFTGVKFTD
                     FSKHPNIRHEANGVVSTAAGAYQFLYGTWRNLQRRYSFPDFSPSNQDLGCIALIAGRK
                     ALDVVMQGKISEAIHLCRIEWASLPGSPHAQPTANKKMIMEKYELYLAEEKEGKTSLH
                     ATTEK"
BASE COUNT          275 a          162 c          183 g          224 t
ORIGIN      
        1 cgatgattcg cgaaccagtc atgcgctggt gaagcataat tatcagcgcc tgctggataa
       61 aattgatagt ggaagcgatc gttactctcc aatggaatac tggagagcac ttcataatcc
      121 cgactatcgt gacgttcttc agaaaacaat cgttaaacat cccagcgact ggtacttcaa
      181 aaaagacgat gcaatctggc aaccatttct taatgcgctg aaaaaagatg ctccggaatg
      241 gaagaaatac agcgaagatt ttcttgataa gatggcctgg atgcaggatg tgaccacgga
      301 gaaactgggc ccttcgttgt ggcatatgca tccaataatg tttttagcct ccctaattca
      361 acctaatgaa gatattagaa ttcttcgttt acgtgcattt ctacgaatga tccgcatcgg
      421 tgaagggaca attcaggaag acggatacag aacgatgttt actggtgtaa aatttacaga
      481 ttttagtaaa caccccaata tcaggcatga agctaatggt gttgtttcga ctgctgctgg
      541 agcttatcaa tttttatacg gaacatggag aaatttgcaa cgtagatatt catttccaga
      601 tttcagtccg agcaatcaag atctcggttg tatagcgctc attgcaggtc gaaaagcatt
      661 agatgtagta atgcagggta agattagtga agccatacat ttatgtcgta ttgagtgggc
      721 cagtttaccc ggttcaccgc atgctcaacc aactgcaaat aaaaaaatga ttatggaaaa
      781 atatgaactc tacttagctg aagaaaaaga aggtaaaaca agtttacatg ccaccacaga
      841 aaaa
//
LOCUS       DAZGHF010000422          839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGHF010000422 DAZGHF010000000
VERSION     DAZGHF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.426"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>839)
                     /locus_tag="ACHK3A_005325"
     CDS             complement(<1..>839)
                     /locus_tag="ACHK3A_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO8743241.1"
                     /translation="LEDLIESPEISGVDSGDKNIEEQSESELAPEQKRTGIAKMLMTT
                     PVLTLIFDRVRYDEIELQPDFQRKDRIWPDDKKSKLIESILMKLPLPGFYFGEKPNGN
                     WVVIDGLQRTTTICDYMSGHFSLKGLSILEHLNGKSFKDLTRTEQRDIREYQITAYQI
                     ELNDDSSELVVELFHRINTYGVKLSSQEIRSALNKGNSVTFLRYLASLETFKKATQFK
                     VKPDRQKDMELCLSALAFMVLGYNNYGQHSYDHFLCSAMQKLNNYPLSIINKEEIDAG
                     TAL"
BASE COUNT          250 a          167 c          127 g          295 t
ORIGIN      
        1 taagagctgt tccagcatca atctcttctt tattaatgat gcttaatgga taattattta
       61 gcttctgcat tgcactacat aaaaaatgat catacgaatg ttggccatag ttattataac
      121 caagtaccat aaaagcaaga gcggacaaac agagctccat atctttttgc ctgtccggct
      181 ttactttaaa ttgcgtcgct tttttgaacg tttctaatga agctaaataa cgtaaaaagg
      241 ttacgctatt gcctttatta agtgcagatc ttatttcttg actacttaac tttacaccat
      301 aagtgtttat tctatggaat agttctacaa ccaattcaga actatcatca tttagttcta
      361 tttggtaagc cgtaatttga tactctctaa tgtctcgttg ctcggttcga gtaagatctt
      421 taaaactctt tccatttaag tgctctaaaa tagagagccc tttcaatgag aaatgaccac
      481 tcatataatc acaaattgta gtcgttcttt gaaggccatc aataactacc cagttaccgt
      541 ttggcttttc accaaagtaa aaccccggta atggaagctt cattaatatt gattcaataa
      601 gttttgactt tttgtcgtca ggccaaattc ggtcttttct ctgaaagtca ggttgtaact
      661 caatttcatc atatcttaca cgatcaaaaa ttagtgttaa aactggtgta gtcataagca
      721 tttttgcaat gcccgtacgt ttttgctcag gtgctaattc tgattccgat tgttcctcaa
      781 tattcttatc acctgaatca actccactta tctcagggga ctcaattaaa tcctctaaa
//
LOCUS       DAZGHF010000423          824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGHF010000423 DAZGHF010000000
VERSION     DAZGHF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.427"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="ACHK3A_005326"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="ACHK3A_005326"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO8743242.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          214 a          208 c          184 g          218 t
ORIGIN      
        1 taacgcttat aaatgtttaa tcaggcaata tttagatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaatgca ggtttcaatg cacaaacgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcacgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggtgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ctcatgagta ccactggttt caatctggca gctaaaacca
      361 ttcttttcga gcagatcagt cagtggtagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt gtatccctgg cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgcca caacaatcat gtcttattaa cattctgtta caggcaggtt
      781 taaagaaaaa tgagaaacag atcgttaata ataaaaggga gtaa
//
LOCUS       DAZGHF010000424          823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGHF010000424 DAZGHF010000000
VERSION     DAZGHF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.428"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>823
                     /locus_tag="ACHK3A_005327"
     CDS             <1..>823
                     /locus_tag="ACHK3A_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO8743243.1"
                     /translation="YGDNVISIDIYNQKNINEVMQVLSPIYKSLSYSKTYIFNGGQTP
                     YDDKPLSVTGIDALGLSINKITTNNMEFNGDVAIVSEDSPYLHNGIVFLNGIPFKIIG
                     VIPKMKMDFLDSLGLSKYQSNNQLFVPLSTLFRFKLSSNIDNVKIVLSDKVGENDINT
                     TKKTLGEEKIPEYNIITALDVRTTVDKVLNRFSILTNTIYIMLTLFAAVICIVLCKRT
                     FSSRSTEFALKIIHGIKQNSIILVVIIESMITLVICLFNSLVASYFIMLWMSQMLS"
BASE COUNT          292 a          132 c          131 g          268 t
ORIGIN      
        1 cttatggtga caatgttatt tctattgaca tctataatca aaaaaacatt aatgaggtaa
       61 tgcaagtatt atctcctata tataaatcat tatcatactc aaaaacttat atattcaatg
      121 gcggtcaaac accttacgat gacaaacctt tatcagtgac aggtattgat gcgctaggtt
      181 tatccataaa taaaataacc accaataaca tggaatttaa tggagatgtt gctattgtta
      241 gtgaggatag tccatattta cacaatggga ttgttttcct taatgggata ccgtttaaaa
      301 ttattggcgt aattccaaaa atgaaaatgg acttcctgga cagtcttggg ttatcgaaat
      361 atcagagcaa caatcagctc tttgtcccct taagtacatt attcagattc aagctaagca
      421 gtaatattga caatgttaag atagtgttaa gcgataaagt tggcgaaaat gatattaaca
      481 caacaaaaaa aacgcttggt gaggaaaaaa taccagaata caatattata acagctcttg
      541 atgtaagaac caccgtcgac aaagtgttga atagattctc catattaaca aacactatat
      601 atataatgtt gacattgttt gctgcggtta tatgcattgt gttgtgtaaa cgaacatttt
      661 cttcccgaag cacagaattt gcactaaaaa ttatccacgg tataaaacaa aactcgataa
      721 ttctcgttgt catcatagaa tcaatgatta cattagtaat ttgtttattc aactcattgg
      781 tcgcttctta ttttattatg ttatggatgt ctcaaatgct atc
//
LOCUS       DAZGHF010000425          821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGHF010000425 DAZGHF010000000
VERSION     DAZGHF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.429"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>821
                     /locus_tag="ACHK3A_005328"
     CDS             <1..>821
                     /locus_tag="ACHK3A_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO8743244.1"
                     /translation="LGQKCRVVLVGDCYQQIYRFRGANNALSHPALKNADRLWLTQSF
                     RFGPAVARMANLLLQREGETREVKGCGGDDEVLLKCHAREHLQGHYTVLSRTVAGVIG
                     TALMAAMKGQKVYWVGGIEGYRTGELEDLYWFQVDMPERMHSDRLRRDYRNFEEYKYI
                     AKSTKDVEMNQSLRLLELCFPLPKKLELLRQYTVTNEQDADITVSTAHRSKGLEWPVV
                     MLDEDFQDITDPLMTEAERHDETCLLYVAVTRALRTLVLNDLMHQLAEEQQKTEG"
BASE COUNT          201 a          163 c          261 g          196 t
ORIGIN      
        1 tctggggcag aagtgtcggg tggtccttgt tggagattgt tatcaacaga tttaccgctt
       61 tcggggtgcg aataatgcgc tttcccatcc tgcactgaag aatgcggacc ggttgtggct
      121 gacacagagt ttccgttttg gtccggcggt ggctcgtatg gcaaatctgc ttctgcagcg
      181 tgaaggtgaa acacgagagg tgaaaggttg tggtggtgat gatgaggtat tgctgaaatg
      241 ccatgcgcgt gaacatctgc aggggcatta cacggtactc agtcgtactg tcgcgggtgt
      301 gattggtact gcgctgatgg cggcaatgaa agggcaaaag gtttactggg tggggggaat
      361 cgaaggttac aggaccgggg aacttgagga tctctactgg ttccaggttg atatgccgga
      421 acggatgcat tcagacaggc tgcgccggga ctacaggaat tttgaagaat ataaatatat
      481 tgcaaaatcc acgaaggatg tggagatgaa tcagtccctt cggcttctgg agttgtgttt
      541 tccgttaccg aaaaaactgg aacttctccg gcagtatacg gtaacgaatg agcaggatgc
      601 agatatcacg gtatcaacgg cacaccggag caaggggctg gagtggccgg ttgtaatgct
      661 ggatgaggat ttccaggata tcacggatcc gctgatgacg gaggccgagc gtcatgatga
      721 aacctgtctg ttatatgtgg ctgtcacccg ggccctcagg acgctggtac tgaatgacct
      781 gatgcaccaa ctggcagaag aacagcagaa aactgaaggt t
//
LOCUS       DAZGHF010000426          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGHF010000426 DAZGHF010000000
VERSION     DAZGHF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.430"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>819
                     /locus_tag="ACHK3A_005329"
     CDS             <1..>819
                     /locus_tag="ACHK3A_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO8743245.1"
                     /translation="VRTRRDEIDWVLTDKTHQTGSDISPEALKIRLTERYFTDFGNAW
                     LNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSPLVALMNTLNYQGKTGQKGEALADSVV
                     DSAKKLLDKNKNGRQFIEQAQGPEGPLDGVFGPLTGLMEGKEGAGGNGNLSFRSWLAR
                     VTQMRLKLQQVTSAPDPQAMSQMLARAVFQGKAIDLTDTRDYGSLVAASLGQEWSGFG
                     QSLFVQPLDLAWRQVLVPAAGSLNARWQTTIVDQWNKAFAGRYPFKATGSDASLP"
BASE COUNT          181 a          209 c          261 g          168 t
ORIGIN      
        1 gtcagaacac gccgcgatga aattgactgg gtactgacgg ataagacgca tcagacaggg
       61 agcgatatct ctccggaggc gctgaagatc cgcctgactg agcgctattt taccgatttt
      121 ggcaatgcct ggctgaacat gctcaacagc atacagtggc atgaacccac gtcactttcc
      181 ggagccattg cccagttgag tctgctcggt gatgtccgtc agtctccgtt agttgcactg
      241 atgaacacgc tgaactacca ggggaaaacg gggcagaaag gtgaggcact ggctgattca
      301 gtcgtggatt cagcgaaaaa gctgctcgat aagaataaaa atggcaggca gtttattgaa
      361 caggcacagg gccctgaagg cccgctcgat ggggtatttg gaccgctgac agggctgatg
      421 gaggggaaag agggggcggg tggcaacggt aatctgagct tccggtcctg gctcgcccgt
      481 gtaacccaga tgcggctgaa acttcagcag gttaccagcg ccccggatcc gcaggcgatg
      541 tcgcagatgc tggcccgggc cgtgtttcag ggcaaagcca tcgatctgac ggatacccgc
      601 gattacggca gccttgttgc agcaagtctg gggcaggaat ggagtggatt tggtcagtcg
      661 ttgtttgtgc aaccgctgga tctggcctgg cggcaggtgc ttgtcccggc tgcgggcagc
      721 ctcaatgccc gctggcagac aaccatagtc gatcagtgga ataaggcttt tgcggggcgg
      781 tacccgttta aagccaccgg cagtgatgcc tccctgccg
//
LOCUS       DAZGHF010000427          807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGHF010000427 DAZGHF010000000
VERSION     DAZGHF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.431"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..365
                     /locus_tag="ACHK3A_005330"
     CDS             <1..365
                     /locus_tag="ACHK3A_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFO8743246.1"
                     /translation="PEEAKAEVAEIAAEIADAAAEVAELAEEVKAAPEDEVKAEAFTA
                     AKAKLEDAIKAFSTITPEKGERYRRFAARRRDDTDKEEIKAIAEKLNDLTTKLSATGI
                     TPRPAGTPAESEKPFDFV"
     gene            377..>807
                     /locus_tag="ACHK3A_005331"
     CDS             377..>807
                     /locus_tag="ACHK3A_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFO8743247.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGSENEFFNRIQNFVNRQIALDILR"
BASE COUNT          221 a          240 c          216 g          130 t
ORIGIN      
        1 cgccggaaga agcaaaagcc gaagtcgcgg aaatcgccgc cgaaatcgcc gacgccgccg
       61 cagaggtggc agagctggca gaagaagtga aagccgcccc ggaagacgag gttaaggccg
      121 aagcctttac cgctgcaaaa gcgaagctgg aagacgccat caaagccttc agcaccatca
      181 caccggaaaa aggcgaacgt taccgccgct ttgccgcccg tcgccgggac gacaccgaca
      241 aagaagaaat caaagccatt gccgaaaaac ttaacgacct gaccacgaag ctgtcagcca
      301 ccggcatcac gccacgcccg gctggcacac cggcagaaag cgaaaaaccg tttgactttg
      361 tttaaggaca cgcaacatgc aactgaacca gaaaaccgaa tcactgcttc gtaagtacgc
      421 tgccgggctg ggcgcggcaa acggggttga cgacgtcaca cgctattttg cgctgtctga
      481 cccgaaggag accgcgctgc gtgacgccat catgcactcc gacgagtttt tatcaaaact
      541 gccgaacgtg ctggatgttg aacaggtgac aggccaggtg gttaccaccg gcgttccggg
      601 cctccatacc ggacgtaaaa aaaatgcccg cttcaccctt ccgctgggtg ttaccgggaa
      661 tgaatacaag ctggctgaaa ccgattccgg cgcccacctc ctgtacacca cactggtcac
      721 ctgggcgaac gcaggaagtg aaaacgagtt ctttaaccgc attcagaatt tcgttaacag
      781 acagattgcg ctggatatcc tgcgcat
//
LOCUS       DAZGHF010000428          795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGHF010000428 DAZGHF010000000
VERSION     DAZGHF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.432"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..382)
                     /locus_tag="ACHK3A_005332"
     CDS             complement(<1..382)
                     /locus_tag="ACHK3A_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743248.1"
                     /translation="MGRKLIWRAVASAPGHEEVHVFVDASDRAEAKKHLYQRLAEEWR
                     CSVNRITFHNMWNENELREMSTGPREPAGLPLLESGGGNGGVFYDHEPLLLIASRRLR
                     ASLETALIVLQKWTVCVGCGYSGVR"
     gene            complement(367..>795)
                     /locus_tag="ACHK3A_005333"
     CDS             complement(367..>795)
                     /locus_tag="ACHK3A_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO8743249.1"
                     /translation="PVLPDVREFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTA
                     LARQTLAELTGIINAGRYSKVLPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSA
                     RCSDSALVTMHSLACELLPEWRAFFDALLENGGKSLWAEN"
BASE COUNT          183 a          238 c          200 g          174 t
ORIGIN      
        1 cccgtacccc ggaataacca cagcccacgc acaccgtcca cttctgcagt acgatgagtg
       61 cagtttcgag tgatgcgcgg agacgccggg aggcaataag gagcaacggc tcatggtcat
      121 aaaaaacccc gccgttaccc cctccggatt caagcagcgg gagtcctgca ggctcccggg
      181 gacctgttga catttcccgt aattcgtttt cattccacat attgtggaaa gttatccggt
      241 tcactgaaca acgccattcc tccgccagac gctgatacaa atgctttttc gcttctgcgc
      301 ggtcagatgc atcaacaaac acatgcactt cctcgtgtcc gggtgctgaa gccacggcac
      361 gccatatcag ttttctgccc acaggctttt ccctccgttt tccagcagag catcaaaaaa
      421 cgcacgccac tccggcaaca gttcacaggc cagactgtgc atggtgacca gcgcagaatc
      481 gctgcaacgg gcagacactt tttccagacg atgcaccggc tgaccggctg cgtgcccctg
      541 tttataaata ttcaggtcgt aaatctgtgt tgaaagaagg gtgaccgcac cttcaggcag
      601 cactttgctg tagcgaccgg cgttgataat gcctgtcagc tctgccagtg tctgtctggc
      661 cagcgccgta taatccatgc agttaaccag ggtgcggact ttcggcaggg gaatacccag
      721 ttcacggtag ggcataaggt tttccatgac attgaccgtg ccgcggatga actcacgcac
      781 atccggcaga accgg
//
LOCUS       DAZGHF010000429          795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGHF010000429 DAZGHF010000000
VERSION     DAZGHF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.433"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            173..248
                     /locus_tag="ACHK3A_005334"
     tRNA            173..248
                     /locus_tag="ACHK3A_005334"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ile,seq:cat)
BASE COUNT          229 a          156 c          178 g          232 t
ORIGIN      
        1 taacatgctt gttgactgcg agtatcgact ttgcgaatgg aagcggtgtc gggatttatg
       61 tcgcatgtat tgacgaccca ggatggatga gaacgcataa aaatctgtta tttacatcat
      121 aagatcatca tgtttattgc gtggtttata agtattgtta ttgcatactt aaggcccttt
      181 agttcagtgg ttagagcagg cgactcataa tcgcccggtc gctggttcaa gtccagcagg
      241 ggccaccaaa tcatcattca tgtatcaaga agagttaagg gtacaagggg tactcgatgg
      301 cggtctatat ttcatgacta acgccattta aaggaagaag tgcggtgggt ggtttcgaat
      361 gagggctggt gttttggtac gtggcacgcc actgcaatgt atttatccat cagtactcat
      421 tcaaaagtac ctggaaaaaa tgcccgctat gccaagcggg caaattgctt acatgtggaa
      481 ttaagtaatt aacgataaag ccagagtatt atcatttatc atgcctcttc atttaatcag
      541 gttaattact caaatcactg aactattctc tgttcgctca ctgatgataa gtgaagtgtc
      601 acaaccacga tttgtatgtg gaccaatgcc gtagagtaag gatgaaatgt ctctgccacg
      661 ccttaaatat ctactactga ccagaacacc cgccgcaggc agatgagtct tatgagcgtt
      721 acaggagtct ggctttgctc attaattaac gctatttaga tataaatttc taacactcga
      781 aaaacgaatt ccact
//
LOCUS       DAZGHF010000430          788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGHF010000430 DAZGHF010000000
VERSION     DAZGHF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.434"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            173..391
                     /gene="tdcR"
                     /locus_tag="ACHK3A_005335"
     CDS             173..391
                     /gene="tdcR"
                     /locus_tag="ACHK3A_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO8743250.1"
                     /translation="MSKFSNFIINKSFSVINNAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            647..748
                     /locus_tag="ACHK3A_005336"
                     /pseudo
     CDS             647..748
                     /locus_tag="ACHK3A_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          263 a          141 c          145 g          239 t
ORIGIN      
        1 gttaaagtat aaaaatcagc ataaaccctg atttttgtca aaaaaaatga cggggataac
       61 catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg ggctgagacc
      121 atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt taatgagcaa
      181 gttctcaaat tttattataa ataaatcatt ttcagtgata aataatgcgg cacgtcacat
      241 tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta aaatttcgaa
      301 tacaggtatc gatcatctcg aacaattgat taacgtcaac tttttctctt ctgacaggac
      361 gtcattttgt gaatgcaatc gttttccata aattcctctc ccctcatagg cgacgaatag
      421 cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa ggtcagacga
      481 agacaacaag ggggattctc actgtgcttt acatcagcgt aggccataga atgttgcttc
      541 gttgactgta atgccgggta aatgagcatt ttttgatagt gcgaaagaac cctgcgagga
      601 aaataagcat ctattattgt ttgtcatgat gacaaggcag gcatcaatga aaggcttccc
      661 aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta acaatcacaa
      721 cagaaatatt gattattgga cggtaaaaat ttaggttata acgtccaaat gattcaacga
      781 tcggacaa
//
LOCUS       DAZGHF010000431          784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGHF010000431 DAZGHF010000000
VERSION     DAZGHF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.435"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..324
                     /locus_tag="ACHK3A_005337"
     CDS             <1..324
                     /locus_tag="ACHK3A_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO8743251.1"
                     /translation="ALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTASYARDRYL
                     TEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKHDKAIYTP
                     EAEHE"
     gene            317..679
                     /locus_tag="ACHK3A_005338"
     CDS             317..679
                     /locus_tag="ACHK3A_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO8743252.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYEDLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
BASE COUNT          247 a          187 c          189 g          161 t
ORIGIN      
        1 gcgctgaacg gcgtaaatac cagccagata atgtggtggc tgtctggccg aaaatgggta
       61 ttcaacgaac aacgcgatcc ggagaaaaca ccccgctggc gcacggcctc ttatgcccgt
      121 gacagatacc tgaccgaaga agcagtacag atcactccac acgggaaagc aacattcacg
      181 aaattcaccc ccgtattgct ggaaaaagga tgccaccgtt tataccagtt ttacatgaac
      241 ggtgaactcc ctatgaagaa aaactggaac ggtgaattta aacacgacaa agctatttac
      301 acaccggagg cagaacatga atagcactac tgcggcaatc atgaactacg acccgaacat
      361 gacattatgc ggccgcatgg caagacagac ggttcgtctg acactcgggc aatgggagta
      421 ccgcgaaaca tttgaagtgg acgttgtcgg caatctgacg ggactggatg taataacctg
      481 cgcaattgaa aacctttatg aagacctgcc gtatgaagaa gcacaggatg aatataccgg
      541 tgaaactaac attatggcca caatacagat cggtgatctt gcatgtacgg atgaggacat
      601 gctcggcgaa gcatggctgg caggtatgct tattgcagca gaaattatca gcattgaacc
      661 agcaggaaca ctttcataac ggagatcgcc atcagatgat tatttcattc accgattacg
      721 gcgaacaggc aaaactgatt atcaccagtt cccgcctgac attgcgaaaa cataatcgtg
      781 tcgt
//
LOCUS       DAZGHF010000432          779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGHF010000432 DAZGHF010000000
VERSION     DAZGHF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.436"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            145..741
                     /gene="fimE"
                     /locus_tag="ACHK3A_005339"
     CDS             145..741
                     /gene="fimE"
                     /locus_tag="ACHK3A_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO8743253.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          211 a          147 c          185 g          236 t
ORIGIN      
        1 tttttgtatg atatttaatg taattgactg atagccacat cacttcgtgt gtggttatct
       61 ttttatctat tgggctaatt ttgaccgatt gaggtttcct ataggtattc attcaaatat
      121 atctcagtta ggagtactac tattgtgagt aaacgtcgtt atcttaccgg taaagaagtt
      181 caggccatga tgcaggcggt ttgttacggg gcaacgggag ccagagatta ttgtcttatt
      241 ctgttggcat atcggcatgg gatgcgtatt agtgaactgc ttgatctgca ttatcaggat
      301 cttgacctta atgaaggtcg aataaatatt cgccgactga agaacggatt ttctaccgtt
      361 cacccgttac gttttgatga gcgtgaagcc gtggaacgct ggacccagga acgtgctaac
      421 tggaaaggcg ctgaccggac cgacgctata tttatttctc gccgcgggag tcggctttct
      481 cgccagcagg cctatcgcat tattcgcgat gcaggtattg aagctggaac cgtaacgcag
      541 actcatcctc atatgttaag gcatgcttgc ggttatgaac tggcggagcg tggtgccgat
      601 acccgtttaa ttcaggatta tctcgggcat cgaaatattc gccatactgt acgttatacc
      661 gccagtaatg ctgctcgttt tgccggatta tgggaaagaa ataatctcat aaacgaaaaa
      721 ttaaaaagag aagaggtttg atttaactca ttgataataa agttaaaaaa caaataaat
//
LOCUS       DAZGHF010000433          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGHF010000433 DAZGHF010000000
VERSION     DAZGHF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.437"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>778
                     /locus_tag="ACHK3A_005340"
     CDS             <1..>778
                     /locus_tag="ACHK3A_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO8743254.1"
                     /translation="ENIINLLKSSIVIKVIIVDNGASKSLDELKSIPDVIIKHLGQNR
                     GIATAQNVGLRISLSLCSKYALLLDQDSEIETDLVSKLLDGYITLEQKGIKLAAVGPR
                     PFDLFENKKMKANIQRDIQIFEGFSLTKQIIASGKLINLEVLDQVGFMEDDLFIDGVD
                     HEWCWRAGRLGYKIAIIENAIMKHRLGDARGNFLGFTYKIGSPIRLYYQFRNILVLSR
                     RDYVPLYWKLRNLIGMFLKFIIFSLKESDSKIRRYYMIKG"
BASE COUNT          272 a           99 c          144 g          263 t
ORIGIN      
        1 aggaaaatat aattaatctt ctaaaatcat caattgttat caaagttata attgttgata
       61 atggtgcatc aaaaagttta gacgaattaa aaagtattcc agatgttata attaagcacc
      121 ttggtcaaaa ccgtggtatt gcaacggcac aaaatgtcgg gctaagaatt tctctatctt
      181 tatgctcaaa gtatgcgcta ttactggatc aagattcaga aattgaaaca gatttagtgt
      241 caaaacttct cgacggttac attacattag aacaaaaagg catcaaactt gccgctgttg
      301 gtccccgacc gtttgattta ttcgaaaaca aaaaaatgaa agcaaacatt caaagagata
      361 ttcaaatatt tgagggtttt tctttgacga aacagataat cgcttcaggt aagctaatta
      421 atttggaagt tttagatcaa gtcggtttta tggaagatga tttatttatt gatggtgttg
      481 atcatgaatg gtgttggcgt gctggaagat taggatataa aattgcaata atagaaaatg
      541 ctattatgaa acatcgatta ggcgatgcac gtggtaattt tttaggattt acttataaaa
      601 ttggttcgcc tattagacta tattatcaat ttagaaacat cttagttcta agtcgaaggg
      661 attatgttcc tttgtattgg aaattaagaa acttgatagg tatgtttctg aaatttatta
      721 tcttttctct aaaagagagt gattcaaaaa ttagacgcta ttatatgatt aaaggctt
//
LOCUS       DAZGHF010000434          776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGHF010000434 DAZGHF010000000
VERSION     DAZGHF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.438"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>776
                     /locus_tag="ACHK3A_005341"
     CDS             <1..>776
                     /locus_tag="ACHK3A_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO8743255.1"
                     /translation="HLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAG
                     IYGAAGHSSVDVKDDDASRAGTVRDDAGSLGGYLNLTHTSSGLWADIVALGTRHSMKA
                     STDNNDFRARGWGWLGSLETALPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFG
                     YGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHGVSELPVNWWVQPSVIRTFSS
                     RGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENIT"
BASE COUNT          161 a          221 c          238 g          156 t
ORIGIN      
        1 catctcggtc acgataacaa cggcggtatt gcccgtgggg ccacgccgga aagcagcggc
       61 agctatggct tcgtccgcct ggagggtgac ctgctgagaa cagaggttgc cggtatgtct
      121 gtgaccgcag ggatatatgg tgctgcaggc cattcttccg ttgatgttaa ggatgatgac
      181 gcctcccgtg ccggcacggt ccgggatgat gccggcagcc ttggcggata cctgaatctg
      241 acacatacat cctccggcct gtgggctgac attgtggccc tgggaacccg ccacagcatg
      301 aaagcgtcaa cggacaataa cgacttccgc gcccgggggt ggggctggct gggttcactg
      361 gaaaccgctc tgcccttcag tatcactgac aatctgatgc tggagccgca actgcagtac
      421 acctggcagg gactctccct ggatgacggc caggataacg ccggttatgt gaagttcggg
      481 tatggcagtg cacaacatgt gcgtgccggt ttccgtctgg gcagccacaa cgatatgacc
      541 tttggtgaag gcacctcatc ccgtgacacc ctgcgcgaca gtacaaaaca cggtgtgagt
      601 gaactgcctg tgaactggtg ggtacaacct tctgttatcc gcaccttcag ctcccggggg
      661 gacatgagca tgggtacagc tgcagccggc agtaacatga cgttctcacc gtcccggaat
      721 ggcacgtcac tggacctgca ggccggactg gaagcccgtg tccgggaaaa tatcac
//
LOCUS       DAZGHF010000435          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGHF010000435 DAZGHF010000000
VERSION     DAZGHF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.439"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>767
                     /locus_tag="ACHK3A_005342"
     CDS             <1..>767
                     /locus_tag="ACHK3A_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO8743256.1"
                     /translation="EGIVITTVRSRGSRKSRFTLTFEGIPYSETVCWRPALLSRPVIS
                     GSLPARIESHEKNDIYAHLDDQGRYRVKLDFDRNSTERGYAYLWLRLAKPCAGDTYGF
                     HSPLIDGTEVAIVFDGGDPDRPYIAYALHDSEHPDHVTSDNHTRNVWRTPANNKLRME
                     DKRQEEHIKLATEYGKTQLNMGHLVNSRREKRGAGFELRTDEHGAVRAAKGLFLTADA
                     QARAQGPVLEMAAAISQINQANSQMQALNSAAEAAGAL"
BASE COUNT          179 a          207 c          236 g          145 t
ORIGIN      
        1 gaaggcatcg ttatcaccac ggtgcgttcc cgcggttcac gcaaaagtcg ctttacgctg
       61 acgtttgagg ggatccccta cagtgaaacg gtctgctggc gtccggcact gctcagtcgc
      121 ccggtgatat ccggctcgct tccggcgcgg attgagagtc acgagaagaa cgatatctac
      181 gctcatctgg acgatcaggg gcgttaccgg gtgaagctgg actttgaccg caacagtaca
      241 gaacggggtt acgcttatct gtggttgcgt ctggctaaac cctgtgccgg tgatacgtac
      301 ggtttccact caccgcttat cgacggaacg gaagtggcca ttgtatttga cggcggagat
      361 ccggatcgtc cctacatcgc ttatgccctg catgattcag agcatccgga tcatgtcacc
      421 agtgacaatc atacgcggaa cgtgtggcgc acgccggcga acaataagct gcggatggag
      481 gataagcgcc aggaagagca tatcaagctc gcaacggaat atggcaaaac acagctgaac
      541 atgggacacc tggtcaacag ccggcgtgaa aagcgaggtg ccggttttga actgcgaacg
      601 gatgagcatg gggctgtacg tgcagcaaaa gggctgttcc tgacggcaga tgcccaggcc
      661 agagcccagg ggccggtgct ggaaatggcg gccgcgataa gtcagattaa ccaggctaac
      721 agccagatgc aggccctgaa cagcgctgcc gaagccgccg gcgccct
//
LOCUS       DAZGHF010000436          764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGHF010000436 DAZGHF010000000
VERSION     DAZGHF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.440"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>764
                     /locus_tag="ACHK3A_005343"
     CDS             <1..>764
                     /locus_tag="ACHK3A_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8743257.1"
                     /translation="AQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDIRTDAR
                     LDEETVAFLTTTDEGKRINSHLYHDEERRIRLEKLARHKHENPPTLVSEAMAELRALP
                     PFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVNERFAT
                     PAFKATAARERLDELLMLPQLSRDEIQRLATLTATTFSTALERACDEVIEKTGRGDDD
                     LLTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRT"
BASE COUNT          180 a          248 c          209 g          127 t
ORIGIN      
        1 gcacaactgg cgtacccgtg gaacgccccg ctaccggtta tcgatcctga aacgttcggc
       61 agagcgcagc caaccgcatt acgccaggcc attcagagct acatcaacga ggatattcgc
      121 actgacgcca gactggatga agaaaccgtc gccttcctga caaccacaga cgaagggaag
      181 cgaattaatt cgcatcttta ccacgacgaa gagcgtcgga tcaggctgga aaaactggcg
      241 cggcacaaac acgagaatcc gccaacactg gtatctgaag ccatggcaga gctgcgcgcc
      301 ctgccgccgt ttcttgccgg tccgctcatc cgtgacctgg cgcgcctgag ccgcaaacag
      361 gagagcgcac gccgggaagg gcttaagaat aacagcgcat atgcggcgga taacttcgtt
      421 cgtcacgggc tgcgtgcccg cctgaaacgt atcgcccgtg tgaatgaacg ctttgccacc
      481 ccggccttta aggccaccgc cgcgcgcgaa cggctggatg aactgcttat gctgccacag
      541 ctgagccgtg acgaaatcca gcgccttgcc accctgaccg ccaccacgtt ttccaccgca
      601 ctggagcgcg catgtgatga agtcatcgaa aagaccggca ggggtgacga tgacctgctc
      661 acctggttac tgacctacca gccactggca cacatggcgt taaaactggg tatcacgcca
      721 ccctgctggc cgtcgctgga aatcaggccc gaccgccgca ccga
//
LOCUS       DAZGHF010000437          726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZGHF010000437 DAZGHF010000000
VERSION     DAZGHF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.441"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            93..701
                     /locus_tag="ACHK3A_005344"
     CDS             93..701
                     /locus_tag="ACHK3A_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO8743258.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
BASE COUNT          170 a          159 c          248 g          149 t
ORIGIN      
        1 gggtatgagg ccgggaaaaa tgcctcactg catcatggtg tgggggagag tgcccgacag
       61 gtggtgccgg gtctgatgat acgggaggcg tcatgaataa cagcttgtct caggcaacca
      121 acggtctgct gatgcaactg gtgatggacc tcaagagcgg ctacctgcgt cgctgcgaat
      181 ctctggggct ggcccgggag gaaatgcaga tgctgcaggg actgaccatt gaggaaattc
      241 attacctgtc aaactctgaa gtgtcggtta tccggctgga cattaatcat gaaaacctgc
      301 tgcgtatgct gcagcaggcc cggacggagc agaaacgtct gcagcgcatt gaccgggcac
      361 tggcgctggg aggctccatt gaactgatgg cgttttactt tggactttcc agtgtggatg
      421 tggcggcgcg ccgtcgcatt gccggtattg atgttcgccc gggacgtggc atcacactga
      481 gtgatgagga aagtgcagag ctgtggcgtt tatggcagaa gagcggcatc acggatgtgg
      541 agagtgcgga cggactggat gtcatgatgc tggcagctga gcaaatgaat atcccgctga
      601 cggcggtatg gcatgccgtg aggggatggc atcagggaac tgcggggttt aatgatgtat
      661 cggccagtcg ccggaaactg aaccgggaaa aagcagaatg acaaggacaa atgacatggc
      721 cgaaat
//
LOCUS       DAZGHF010000438          721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGHF010000438 DAZGHF010000000
VERSION     DAZGHF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.442"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            404..>721
                     /locus_tag="ACHK3A_005345"
     CDS             404..>721
                     /locus_tag="ACHK3A_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743259.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGF"
BASE COUNT          238 a          149 c          151 g          183 t
ORIGIN      
        1 atagctcctc ccccggaata acggagataa tgaaaaatga ataattgcgt tgaagtactc
       61 gataatgtcc actgcgagat aacagaaagc ctgtctttgt tacaggtgat tattgacagc
      121 ggtgatattg gttcaccaga tgcgctaaac atgatgaatt gtcttttccg ctcattatcc
      181 tgtacagcag acaaagcaga aaagcatctg tgccggactg aaaacgaata acattattta
      241 agaacagtaa ttaatttaca gctttattgt cggggatttt tacaacctga aaaggccagg
      301 ctgtatcaca gatatatcgc aacaaacgaa ttgctgagcc acatcttctg gctgcgctca
      361 gagtgaataa aagtaattaa caccggatga acacggagta accatgaaag aagaacctga
      421 aatcattgaa attaattctg aagaattcac agaaaacgaa tggctgacag aaaccgatcc
      481 gcgcgttctg gcgctgacaa tggaaggata catcgtattt ccacctttct tcagtgatgc
      541 ggaatacagt gcaataaata atgccggaat cgaccgtgct tttcataaat acgaactcga
      601 ttgcggccct gtttacagag tctgccgcca cggacaagtt aagaaatgct tcacgcgaaa
      661 aacggccatc atacatctcg ctcattttat gacaacgaag gtattcgaaa gttcaggttt
      721 t
//
LOCUS       DAZGHF010000439          715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGHF010000439 DAZGHF010000000
VERSION     DAZGHF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.443"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..252
                     /locus_tag="ACHK3A_005346"
     CDS             <1..252
                     /locus_tag="ACHK3A_005346"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO8743260.1"
                     /translation="QMRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAA
                     KANFAVAVGQYSQANAEKSISLGASARAIENNSTALGAK"
     gene            341..>715
                     /locus_tag="ACHK3A_005347"
     CDS             341..>715
                     /locus_tag="ACHK3A_005347"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8743261.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEAPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELK"
BASE COUNT          208 a          171 c          159 g          177 t
ORIGIN      
        1 caaatgcgta aaatcagtgt cagttatata tgcaaatcat tatttctcta ctgctcagct
       61 tctttacctg tcttatctgc aaatggtatt cctgataaaa tgatcgtgga acttggtgat
      121 aacagtgcgg caaaagctaa tttcgctgtt gctgttggtc aatattcaca ggcaaatgca
      181 gaaaaaagta tttctttagg tgcatcagca agagctattg aaaataactc taccgcgtta
      241 ggtgcaaagt aagcgtacag cctgaaccgt ctggtcagaa tctgacgaat tagacaaagt
      301 ggtgtccacc aaataagtag tgggaaccaa agtatcagat atgcagaaaa atgtgactcc
      361 cggcaggcga aaaggctgcc ctaattatcc tcccgaattt aaacagcagc tcgttgctgc
      421 ctcctgtgaa cccgggatat ccatctcaaa acttgctctt gaaaatggca ttaacgccaa
      481 tctgttgttc aaatggcgac aacaatggcg cgagggaaaa ctgctattac cttcttcaga
      541 gagcccccag ctacttcctg tgactctcga tgccgctgcc gaacagccag aatcgctcgc
      601 agaggccccg gaaaccctca gtatcagctg tgaggtaacg ttccggcacg ggacgctccg
      661 cttcaatggc aatgtcagcg aaaagctcct gactctgctg atacaggaac tgaag
//
LOCUS       DAZGHF010000440          710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGHF010000440 DAZGHF010000000
VERSION     DAZGHF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.444"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..569
                     /locus_tag="ACHK3A_005348"
     CDS             <1..569
                     /locus_tag="ACHK3A_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO8743262.1"
                     /translation="KHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQGKVVFIGVGKS
                     GIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGETTEILATLPS
                     LKKMGNYLISFTRSHRSSLAISCDLSVEIPVKSEADNLGLAPSCSSTVVLVVGDAVAL
                     ALSELKKFTRADFGLYHPGGALGIKANS"
BASE COUNT          180 a          143 c          168 g          219 t
ORIGIN      
        1 ttaaacattt tatgcgtaac gaacataaag cagttgaaga agttattgat tccccccttt
       61 cggaggtcgc aaatttaatt aaagtattac agtcttgtca ggggaaagtt gtttttattg
      121 gtgtcggtaa gtccggtatt attgcccgaa aactcgccgc aacatttgcc agcactggaa
      181 ctccctcgtt ttttgttcac ggtactgaag ccgtacacgg cgatcttggg atggtggcga
      241 aagatgatgt tgttattctt atttcaaaca gtggtgagac gacagaaatc ctggcaacac
      301 tacctagtct gaaaaaaatg ggtaattatt tgatctcctt tacccgcagt catcgctcat
      361 cgcttgcgat aagttgtgat ttatccgttg aaattcccgt aaaaagtgag gcggataatt
      421 taggtttagc gccgtcatgc tcatcaacag tcgttctggt tgttggtgat gcggtggcgc
      481 ttgcgctctc cgaattgaaa aagtttactc gtgctgattt cggcttatat catccaggag
      541 gtgcgctcgg tattaaagca aactcataat tttagaatat aaaacagtaa ccggtcacgc
      601 catcttttgg ggtggcgttc attgatgtga gtgtcagcta tgaaaaacgt tctggtcacc
      661 gttcccagct tttcagcgcg ttgtgtttct gccagcaagc tgctaagaga
//
LOCUS       DAZGHF010000441          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGHF010000441 DAZGHF010000000
VERSION     DAZGHF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.445"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>709
                     /locus_tag="ACHK3A_005349"
     CDS             <1..>709
                     /locus_tag="ACHK3A_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743263.1"
                     /translation="AQLTDRQKMDLAKERVADEVRAQLATDPELLAVNAMAAKLGDAQ
                     LFNLVTRGTAKTVKSGIVRNATEQGVINGAQNAYSRYQENMALRETAGMDVSPWEGVT
                     DAGIEGTVFGAAMGAPFGAVAGYRGRRQAAEEAAMRDAETVQQDDAAPQPEPVDPVAQ
                     HRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQAKRQAVMNELK
                     AKPRSELLEEYRRLSQKE"
BASE COUNT          182 a          170 c          233 g          124 t
ORIGIN      
        1 gcacagctta ctgatcgcca gaaaatggat ctggcaaaag agcgtgttgc cgatgaggtg
       61 cgcgcgcagc tggcaaccga tcctgaattg ctggctgtga atgccatggc ggcaaaactg
      121 ggtgacgcac aactgtttaa tctggtgaca cgaggcacag cgaagaccgt taaaagcggc
      181 attgtcagaa atgccacgga acagggcgta attaacgggg cacagaacgc ctattcgcgc
      241 tatcaggaaa acatggcatt gcgtgagacc gccggaatgg atgtatcgcc atgggagggg
      301 gtgactgacg cagggattga aggcactgtt ttcggtgctg cgatgggggc tccattcggt
      361 gcggttgccg gatatcgtgg cagacgtcag gccgcagaag aagcagccat gcgtgatgct
      421 gaaaccgtgc agcaggacga cgcagccccg caacccgaac cggttgatcc ggtggcacag
      481 catcgtgaat ccatgcaggg catgaatcgc gagcagcttc tggaacagta tgctgatgcg
      541 gatatggcaa cagagggtga cgcatccgca gctcatcgcc gggaagctgc cagccagttg
      601 ttgaatgaac tggacgaaca ggcgaagcga caggctgtga tgaatgagct gaaggcgaag
      661 ccgcgttctg aactgcttga ggaataccgc agactcagcc agaaagagg
//
LOCUS       DAZGHF010000442          701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGHF010000442 DAZGHF010000000
VERSION     DAZGHF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.446"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            49..144
                     /locus_tag="ACHK3A_005350"
     CDS             49..144
                     /locus_tag="ACHK3A_005350"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO8743264.1"
                     /translation="MEGKQPKTLGGNFTILIAKKDGQVLNVIHTK"
     gene            complement(506..>701)
                     /locus_tag="ACHK3A_005351"
     CDS             complement(506..>701)
                     /locus_tag="ACHK3A_005351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743265.1"
                     /translation="NILPPYSKNTQAFRTLYQPHLDCFPFPNTAARGVIKVRNFQQYG
                     VLLFTAVMHQPVLAIVVITV"
BASE COUNT          231 a          126 c          151 g          193 t
ORIGIN      
        1 gaatttcaga aaccatatag tattacagat gacaataatt gttggaaaat cgagggaaaa
       61 caaccgaaga cgttaggagg aaattttaca atactaatag cgaaaaaaga tgggcaagtg
      121 ttgaatgtaa tacacacaaa ataatccact gtacagtcga ttcaaatatt ttaccaaggt
      181 gtgttttgta acaattttgt gtacaatatg actctacata tcgacgaaaa aaccacttga
      241 tttgacagga acacattcaa aaaaatttct ttattcattt caggagcgat tttcatctgt
      301 tacttgaagt atctgtgaga ttaagtgact ccccctaacg tcgtactaaa tagtaattac
      361 tgtccatacc gggagcagta ttcggcgctc cccacatgca gcagaataac cgcatcagcg
      421 aagatgtatt gtatttttac tatcacaatg tgtacggacg gtttatggaa aagaatgaaa
      481 attagatacg cgatggggcg gcaggttaca cggttatcac tacgatagcc aatactggct
      541 ggtgcattac cgccgtgaac aataggacgc cgtactgctg gaaatttctt acctttatga
      601 ctccccgcgc cgccgtattg ggaaacggga agcaatcaag atgaggctga tataatgtgc
      661 ggaatgcctg agtattttta gaataggggg gtaatatgtt t
//
LOCUS       DAZGHF010000443          690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGHF010000443 DAZGHF010000000
VERSION     DAZGHF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.447"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..203
                     /locus_tag="ACHK3A_005352"
     CDS             <1..203
                     /locus_tag="ACHK3A_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFO8743266.1"
                     /translation="KVSNADIVHHKENNMNLDNVKSPLIIKEYKNEKAILKKIKKLKR
                     NPKAFLIDSKHRSLNLLARFLF"
     gene            275..396
                     /locus_tag="ACHK3A_005353"
                     /pseudo
     CDS             275..396
                     /locus_tag="ACHK3A_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            424..690
                     /locus_tag="ACHK3A_005354"
                     /pseudo
     CDS             424..690
                     /locus_tag="ACHK3A_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
BASE COUNT          207 a          135 c          166 g          182 t
ORIGIN      
        1 aaaaagtttc caatgctgat attgtacatc ataaagaaaa taatatgaat cttgataatg
       61 ttaaatctcc attaataata aaagaatata aaaatgaaaa ggcaatatta aaaaaaataa
      121 aaaaactcaa acgtaatcct aaagcttttc ttattgactc taagcacagg tctttgaatt
      181 tgttagccag atttctgttc taattttgga tagcagaata tgcaatgtta tttttcgaat
      241 cctccataac tgatatatgt gctgttagtg gagtacaaga ataagctcgc gttagcagtc
      301 tgtcagtact gcgacagcgt tgtctgggag ggctttaccc ggtcaaaaca gcattattgt
      361 gcaccccatg tgacggatga actgtgtgct cagggggcat tttactgtaa aaaccgtgct
      421 gcggtcagcc gtaaccgtca ggtgggtgaa cgcaacagtc gcgccattgc ttttcaggca
      481 cgtaccctgc aggcgcatga gcgtcgtatg aacagccagc agcgtcagat taatgagaat
      541 catgaggaaa tgaagcgggc agcagcccag tcagctgcgc tggccggtct tttccagcca
      601 tacagtgtgg gtaaatttaa cgccacagcg gctgtgggcg gacagtgaca ggcaggctgt
      661 tgcggtgggc atgggatatc gctttaacga
//
LOCUS       DAZGHF010000444          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGHF010000444 DAZGHF010000000
VERSION     DAZGHF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.448"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>686
                     /locus_tag="ACHK3A_005355"
     CDS             <1..>686
                     /locus_tag="ACHK3A_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8743267.1"
                     /translation="MWLVRGGKAAQPDGHTLGRLWGALPPDIRLSPHLYLATSSAQGP
                     WWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGRTLTYRREAAGDLAGEITGVTDG
                     AGREFRLVLTTQAQRAEEARTSSLSSSDRPRPLSVSAFPDTLPGTEYGPDRGIRLSAV
                     WLMHDPAYPESLPGAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVAHRYA
                     GRPEMRYRYD"
BASE COUNT          105 a          219 c          252 g          110 t
ORIGIN      
        1 atgtggctgg tgcgcggtgg taaggcagcg cagccggacg gccacacgct ggggcggctg
       61 tggggggcgc tgccgccgga tatccggtta agcccgcatc tttacctggc gaccagcagc
      121 gcacagggac cgtggtggat actggggtgg tcagagcggg tgccgggtgc tgaggacgta
      181 ctgccagcgc cgctgccgcc gtaccgggtg ctgacggggc tggcggaccg cttcgggcgg
      241 acgctgacgt accggcgtga ggccgccggc gacctggccg gggaaatcac cggcgtgacg
      301 gacggtgccg ggcgtgagtt ccgtctggtg ctgaccacgc aggcgcagcg tgcggaagag
      361 gcccgcacct cttcgctatc ttcttctgac cgtccccgcc ctctctcagt ctcagcgttc
      421 cccgacacac tgcccggtac cgaatacggc cccgacaggg gtatccgcct ttcggcggtg
      481 tggctgatgc acgacccggc atacccggag agcctgcccg gtgcgccact ggtgcggtac
      541 acgtatacgg aagccggtga actgctggcg gtatatgacc gcagcaatac gcaggtgcgc
      601 gctttcacgt atgacgcgca gcacccgggc cggatggtgg cgcaccgtta cgcgggaagg
      661 ccggagatgc gctaccgcta cgacga
//
LOCUS       DAZGHF010000445          679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGHF010000445 DAZGHF010000000
VERSION     DAZGHF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.449"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..641
                     /locus_tag="ACHK3A_005356"
                     /pseudo
     CDS             1..641
                     /locus_tag="ACHK3A_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961341.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          233 a          110 c          130 g          206 t
ORIGIN      
        1 atgttgcttc acttcagcca ggtagaacac gtagtgttat ttcagaaacg ccgccaaatg
       61 actgtatgga atcacataat ttatatgaca accaaataga taagatttca cctgtagcta
      121 aaaactcgca gcaagttaaa ggtcactatg gagataaatt taaagaaatg cacatgttca
      181 ttaaccagat gagcaatgca cttcaacagg cttcatcttt gtcagagtca aaggaacaca
      241 ccgtagatat tcaagaaaaa acgaataagt ttgtgcagca ttttcagcgg gtattatttg
      301 ataaaaatgg aaggtcatca gagtttctac ttaattttta tgagtgttgc tataagtttt
      361 taccaagagc gcagcctcaa gataaaatcg atagctataa ttcagcactg caagcttttt
      421 ccatcttttg ttcatctacg ttgacacata ataatgtagg gtttaatttc aaattatttc
      481 cagaagtcaa gctgtctgga gaacatcttg aaacggtatt caaatacaaa aatggcgatg
      541 atgtccggga gatagccaaa attaacattg ctctccaaaa agaagagggt ggtttatata
      601 atttaggtgg attggatttt aagggatgct tcttttctgg atagaacttc agtaactatg
      661 atattaaata tgtgaactg
//
LOCUS       DAZGHF010000446          679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGHF010000446 DAZGHF010000000
VERSION     DAZGHF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.450"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>679
                     /locus_tag="ACHK3A_005357"
     CDS             <1..>679
                     /locus_tag="ACHK3A_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system domain-containing
                     protein"
                     /protein_id="HFO8743268.1"
                     /translation="GSAVADWPEAEKPSFAGLCSALENRLARSGGMEALVPQNSSPRE
                     TGRESAPPDSPQLAAVTSGRDLLDQARLLSRWLSGQPQGWLASHRLMKTVRWDTVDQL
                     PSLDSSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLW
                     QGLSHAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEE
                     DVMYGDEA"
BASE COUNT          128 a          190 c          218 g          143 t
ORIGIN      
        1 ggatccgccg tggcagactg gccggaagcg gagaagccgt cttttgcggg gctttgttcc
       61 gcgcttgaga accgcctggc acgttcaggg ggcatggagg cactggtccc ccagaacagc
      121 agtccccggg agaccgggcg tgaatcagct cccccggact caccacaact ggcagcggtg
      181 acctcggggc gcgacttact cgaccaggcc aggttacttt cccgctggct gagcgggcag
      241 ccgcagggct ggctggcttc tcaccggctg atgaagacgg tgcggtggga tacggtcgat
      301 cagctccctt cgctggacag tagcggacgc acccgcctgg tccccccgaa aaccgggtat
      361 cgcgctcagc ttaagcgact ctggcttcag aaaaactggg ttgagctgat tgaactggcc
      421 agtcagatgt attgcgaagg tgtgaatcat ttctggctcg atcttcagtg gtatctctgg
      481 cagggactca gtcatgctgg tcagccctgg gattcctggt ctgacgctgt cctgtcagat
      541 ctgcggctgt tgcttaaacg tctcccgggc cttgaaggac tggcatggaa tgacggtact
      601 ccctttgctg acgaggtcac cctcgcctgg atcgctgaaa aagtgaatga agaagatgtg
      661 atgtacggcg atgaagctg
//
LOCUS       DAZGHF010000447          678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGHF010000447 DAZGHF010000000
VERSION     DAZGHF010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.451"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>678
                     /locus_tag="ACHK3A_005358"
     CDS             <1..>678
                     /locus_tag="ACHK3A_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO8743269.1"
                     /translation="AERLRSQLVLQQAIARLQHREATGAPWYTRFGLNQDSDTLAALW
                     PLYAKNNAQLMRDATADSLRQQLNAFVQLPPASDARTQGTQHTYDILKSYLMLARPDK
                     ADAGWLAKNALKALPKRRNVPDGIWQNVAPKLLGFYAQNLPAHPEWKIKPDDELISTV
                     RQILLKQIGQRNAESGLYQEMLKRVASNWPDLTLADMTGDTDASTVFSTDEVVPGMFT
                     RQAWEEQV"
BASE COUNT          168 a          184 c          191 g          135 t
ORIGIN      
        1 ggctgaacgc ctgcgcagcc agcttgtact ccagcaggcc attgcccgtc tgcaacatcg
       61 tgaggcaacc ggtgcgccct ggtacacacg atttggtctt aatcaggaca gcgacacgct
      121 ggccgcgctc tggccgctgt atgcgaaaaa taacgcacag ctgatgaggg atgccacagc
      181 agacagcctc aggcagcagc tgaatgcctt tgtgcaactc cctcctgcca gtgatgcccg
      241 tacgcagggc acacagcata cttacgacat actcaaaagt tatctgatgc tggcgcgccc
      301 ggacaaagcg gacgccggct ggctggcaaa aaatgcgctg aaagccttac cgaaacgccg
      361 gaatgtgccg gatggcatat ggcagaacgt cgcaccgaaa ctgctggggt tttatgcgca
      421 aaaccttccg gctcatccgg aatggaaaat taagcctgac gatgaattaa tcagtacggt
      481 ccgtcagatt ctgcttaagc aaattggcca gcggaatgct gagtccgggt tatatcagga
      541 gatgcttaag cgcgtggcca gtaactggcc tgatttaaca cttgcggata tgaccggtga
      601 caccgatgcc tcaacggtat tcagcactga cgaagtcgtt ccgggaatgt ttacgcgtca
      661 ggcctgggaa gagcaggt
//
LOCUS       DAZGHF010000448          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGHF010000448 DAZGHF010000000
VERSION     DAZGHF010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.452"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHK3A_005359"
     CDS             complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHK3A_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFO8743270.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          157 a          187 c          125 g          202 t
ORIGIN      
        1 ccggaggttt ttcaaatgcg cctataaggc tctcttacca gccgcgccct aacaggcgca
       61 cacgatctga catttgcatc caacttcgtt acttacggcc cgtaaacggg ctgcccggat
      121 agggaatcga taactgctct cccattttat cctcttcaag ctggtgcttt atgtaatcct
      181 gtatcttcgc cgtgttctta cccaccgtat cgacgtagta ccctctgcac cagaactctc
      241 tgttcctgta tttgaatttc aaatcaccaa actgctcgta aagcatcaga ctgcttttcc
      301 ctttcagata tcccataaag ccggatacac tcattttggg cgggatctcc acaagcatat
      361 ggatatgatc tgcacagcat tcagcttcaa gaatccgtac gcttttccac tcacacagct
      421 ttctcaaaat actgcctatt gctctacgct tctctctgta gaacacttgt cttcggtatt
      481 ttggcgcaaa aactatgtga tatttacagt tccatcgggt gtgcgctaag ctcttttcgt
      541 tccccattgg gacccccttt tgatttcttg tttgacactt gcagttgcca gaccgcaagg
      601 tgttttaaca aatcaaaagg ggttttaata actggctcaa agctgaaagc tttccggaac
      661 ccccagccta g
//
LOCUS       DAZGHF010000449          669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGHF010000449 DAZGHF010000000
VERSION     DAZGHF010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.453"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>669
                     /locus_tag="ACHK3A_005360"
     CDS             <1..>669
                     /locus_tag="ACHK3A_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO8743271.1"
                     /translation="TDTTVNGGLFTARGGSLAGTTTLNNGATLTLSGKTVNNDTLTIH
                     EGDALLQGGALTGNGRVEKSGSGTLTVSNTTLIQKTVNLNEGTLTLNDSTVTTDVIAQ
                     RGTALKLTGSTVLNGAIDPTNVTLTSGATWNIPDNATVQSVVDDLSHAGQIHFTSART
                     GKFVPTTLQVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSG
                     TGLAT"
BASE COUNT          171 a          203 c          187 g          108 t
ORIGIN      
        1 acggatacca cggtaaatgg cggactgttc accgccagag ggggctcgct ggcgggcacc
       61 accacgctga ataacggtgc cacgcttacc ctttccggga aaacggtgaa taacgatacc
      121 ctgaccatcc atgaaggtga tgcactcctg cagggaggcg ctcttaccgg taacggcagg
      181 gtggaaaaat caggaagtgg cacactcact gtcagcaaca ccacactcat ccagaaaacc
      241 gtcaacctga atgaaggcac gctgacgctg aacgacagta ccgtcaccac ggatgtcatc
      301 gctcagcgcg gcacggccct gaagctgacc ggcagcacag tgctgaacgg tgccattgac
      361 cccacgaatg tcaccctgac atccggtgcc acctggaata tccccgataa cgccacggtt
      421 cagtcggtgg tggatgacct cagccatgcc ggacagattc atttcacctc cgcccgcaca
      481 gggaagttcg ttccgacaac cctgcaggtg aaaaacctga acgggcagaa tggcaccatc
      541 agcctgcgtg tacgcccgga tatggcgcag aacaatgctg acagactggt cattgacggt
      601 ggcagggcaa ccggaaaaac catcctgaat ctggtgaacg ccggcaacag tggtacgggg
      661 ctggcgacc
//
LOCUS       DAZGHF010000450          669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGHF010000450 DAZGHF010000000
VERSION     DAZGHF010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.454"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..256
                     /locus_tag="ACHK3A_005361"
     CDS             <1..256
                     /locus_tag="ACHK3A_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743272.1"
                     /translation="SVTEGFENKDGLLSVLESRYFSDELVQECIDRLRKEYGQGNVPL
                     SVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            260..574
                     /locus_tag="ACHK3A_005362"
     CDS             260..574
                     /locus_tag="ACHK3A_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743273.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKEELWYYNLFINPSADELMNVNRRYGPERTAWLQGFIRNN
                     GA"
BASE COUNT          181 a          138 c          191 g          159 t
ORIGIN      
        1 ctctgtcaca gaggggtttg agaataaaga cgggctgctg tctgtgcttg aatcccgtta
       61 tttcagcgat gaactggtac aggagtgtat tgatcgcctt cgtaaagagt acggacaggg
      121 gaacgtcccg ttgagtgtga ccgtggagcg ttaccggaaa aacgagctgg agaagattga
      181 aacagcgcgt tttccgggca acatatccgt agccataaac gagatcggga taaataccag
      241 caaatacagg aggtaaggta tggctggtaa atgccttgtc tggttctgta acgggatctc
      301 tgaaccgtgt ctgatagcag gaaatatcag tgaattactg gcagatgtta cgatcacgtc
      361 aaattaccgt ggagatgtca catctgaaat cagggatgtt gtacaggagt ggctcaggac
      421 cggcaggggg aacctggcgg atttaaagga agagctctgg tactacaacc ttttcatcaa
      481 tccgtctgca gacgaactga tgaatgtcaa caggcggtat ggccctgagc gaactgcctg
      541 gctgcagggt tttatccgca acaacggggc gtgatacccc gttttcccgt gagggcattg
      601 tacctcacgg gacaatgccc tctttttaca tctgtataaa aaagaggagc gtaaacaatg
      661 tctgaatgg
//
LOCUS       DAZGHF010000451          634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZGHF010000451 DAZGHF010000000
VERSION     DAZGHF010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.455"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>634
                     /locus_tag="ACHK3A_005363"
     CDS             <1..>634
                     /locus_tag="ACHK3A_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFO8743274.1"
                     /translation="QHGITRPLKAVSDTDTERTEKCSRPSQEESAGRGDSGASSAENA
                     LRPEIQPDMYGGKPVFSGDVMEDDAAGAVLASVMTGDTAPGTTDALPASVTHFAVRRD
                     DIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFLAGLAGD
                     APRTVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSV"
BASE COUNT          131 a          158 c          209 g          136 t
ORIGIN      
        1 agcaacatgg tattacccgg ccactgaaag ccgtttctga tactgacaca gagcgcactg
       61 aaaaatgcag tcgaccttct caggaagaga gcgcaggcag gggggacagt ggggcgtctt
      121 ctgctgaaaa tgccctgcgt cctgagatac agccggatat gtacggtggt aagcctgtct
      181 tcagtggtga tgttatggaa gatgatgctg ccggggcagt actggcatca gttatgacag
      241 gagatactgc cccgggaacc actgatgcac ttcccgcgtc ggtgacgcac tttgctgtga
      301 ggcgggatga tatcgaacat ctgcagaatg aagcgttccg tctggcctgg gaactggctg
      361 aatcagccgg ttgtgctgaa gagattgcca tggacaggga aagcgacctg tctgccggtt
      421 tcggggcagc agtggagaaa tgttctcctg tagtggcgtt tctggcgggt ctggccggtg
      481 atgcaccgcg gacagtcctc cccgtgagtc tgaaagacct gcttacaggg ggcagtgcac
      541 cggaagcgtg gccactgctg gatgacgacc atgccgtgaa gctgctgcgt cttctgagtg
      601 tgctgcgccg cctgcgagcc ctgcagcgca gtgt
//
LOCUS       DAZGHF010000452          626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGHF010000452 DAZGHF010000000
VERSION     DAZGHF010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.456"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>626)
                     /locus_tag="ACHK3A_005364"
     rRNA            complement(<1..>626)
                     /locus_tag="ACHK3A_005364"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          122 a          205 c          140 g          159 t
ORIGIN      
        1 agggggccgc cttcgccacc ggtattcctc cagatctcta cgcatttcac cgctacacct
       61 ggaattctac ccccctctac gagactcaag cttgccagta tcagatgcag ttcccaggtt
      121 gagcccgggg atttcacatc tgacttaaca aaccgcctgc gtgcgcttta cgcccagtaa
      181 ttccgattaa cgcttgcacc ctccgtatta ccgcggctgc tggcacggag ttagccggtg
      241 cttcttctgc gggtaacgtc aatgagcaaa ggtattaact ttactccctt cctccccgct
      301 gaaagtactt tacaacccga aggccttctt catacacgcg gcatggctgc atcaggcttg
      361 cgcccattgt gcaatattcc ccactgctgc ctcccgtagg agtctggacc gtgtctcagt
      421 tccagtgtgg ctggtcatcc tctcagacca gctagggatc gtcgcctagg tgagccgtta
      481 ccccacctac tagctaatcc catctgggca catccgatgg caagaggccc gaaggtcccc
      541 ctctttggtc ttgcgacgtt atgcggtatt agctaccgtt tccagtagtt atccccctcc
      601 atcaggcagt ttcccagaca ttactc
//
LOCUS       DAZGHF010000453          626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGHF010000453 DAZGHF010000000
VERSION     DAZGHF010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.457"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..137)
                     /locus_tag="ACHK3A_005365"
     CDS             complement(<1..137)
                     /locus_tag="ACHK3A_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO8743275.1"
                     /translation="MTQRRNFRTGTERHAARFSTSASRSNTHYSLQETHATPDGHLVK
                     QT"
BASE COUNT          128 a          133 c          151 g          214 t
ORIGIN      
        1 gtttgtttaa ccagatgccc gtcaggtgtg gcgtgcgttt cctgcaggct gtagtgtgtg
       61 ttgctgcgtg atgcactggt tgagaaacgg gctgcgtggc gttctgtgcc agtgcggaag
      121 ttgcgtcgct gtgtcatatc ggaaaacccg cttaagttaa aagaattgaa ataacggtgt
      181 attggcattt atgcccagtt gtcttttctt attactgttt cattctgaaa tcttgtctgc
      241 tccattgcat tttttaagga ctcttctgtt tcaaatgtca caacgatgca atgggtcggg
      301 taatggaaat acaaatcgtt gccagcccga acaatggcat ctaagttctg attacagatt
      361 attactgaag cgtcgtcgct tatttttatt ctttgtctga tttccgtgtg tttaatcatc
      421 ttgcccctcc aatgattgcc agttctatat cacgcagctt ttttactgat gcgtttattc
      481 cgtgaaccag ctgctggctt aaataaaaaa agtcggtttt cccattcgtg ccattcttgc
      541 ggtgcgtttt cagtgagtcg atcgcaggct tcatcccacg ctttccggtt gatgtagagt
      601 tcggcgtttc cgccaggctt gagagg
//
LOCUS       DAZGHF010000454          625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGHF010000454 DAZGHF010000000
VERSION     DAZGHF010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.458"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>625
                     /locus_tag="ACHK3A_005366"
     CDS             <1..>625
                     /locus_tag="ACHK3A_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743276.1"
                     /translation="PKLARVEAPTEAVSAGNIADPFRPRYAVNLQLLDENGNPAADTP
                     VYNAVPLPVPMAGSESGMFQFPPPGTLVEVGFVEGRADKPFIRQIMAEGHNLPDVKPG
                     EQLQQQRDGVSQRVTVAGDWERQTDQRIQETSRERVVSADEEKRSLTSRETTIQATDT
                     TTVLGVSTLQAGAVTHITEENYSISTGGHMMVVAGTCERDVEGDTQDT"
BASE COUNT          142 a          199 c          199 g           85 t
ORIGIN      
        1 cccaaactgg cacgtgttga ggcaccgacc gaagccgtca gcgcgggcaa tattgccgac
       61 ccgttcagac cccgctacgc ggtgaatctc cagctgcttg acgagaacgg caacccggca
      121 gcagatacgc ccgtctataa cgccgttccc ctccccgttc ccatggcggg cagtgagtcc
      181 ggcatgttcc agttcccgcc acccggaacc ctggtggaag tgggatttgt ggaaggccgg
      241 gcggataagc ccttcatccg ccagataatg gcagagggcc acaatctgcc cgacgtgaag
      301 ccgggcgagc agttgcagca acagcgcgac ggcgtatcgc agcgcgtgac ggtcgccgga
      361 gactgggagc gccagacaga ccagcggata caggagacat cacgcgagcg cgtggtgagc
      421 gcagacgagg aaaagcggag cctgaccagc cgggagacca ccatacaggc aaccgacaca
      481 accaccgtcc tgggcgtatc cacgcttcag gctggcgcgg tcacccatat cactgaagag
      541 aactacagca tcagcacggg cgggcacatg atggtggttg ccggtacctg tgagcgcgac
      601 gtggaaggcg acacccagga caccg
//
LOCUS       DAZGHF010000455          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGHF010000455 DAZGHF010000000
VERSION     DAZGHF010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.459"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..602
                     /locus_tag="ACHK3A_005367"
     CDS             <1..602
                     /locus_tag="ACHK3A_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HFO8743277.1"
                     /translation="DSGRITTFLKTHLGGLVHQEGNHWVAEPSASQGMVVSPDFLRAI
                     NQLAELSDIVFSQGDAGVHFELMARPSRDVARVQLTLDEQNLDYFNQMESWQSFTWPG
                     NTYYPGVSLSWRSVSSGMQLYASHQGNWGFIRLLDEALITPLDASRTQLVWITADGNP
                     LKFVMRSELGDGPLALLKLQGFTLPTAIFNVAAGTEGVE"
BASE COUNT          130 a          163 c          187 g          144 t
ORIGIN      
        1 gcgactcggg tcggatcacg acgttcctga aaactcatct tggcgggctc gtccaccagg
       61 aggggaatca ctgggtcgct gagccttccg caagccaggg gatggttgtc agtccggatt
      121 tcctccgggc gattaatcag ctggcagagc tgtcagatat cgtgttctct cagggcgatg
      181 cgggggttca cttcgaactg atggcgcgtc cttcacggga tgttgccaga gtgcagctta
      241 cccttgatga acagaatctg gattacttca atcagatgga aagctggcag agcttcacct
      301 ggccggggaa tacttactat ccaggtgtgt cgctcagctg gcggagtgta agcagtggta
      361 tgcaactcta cgccagtcac caggggaact gggggtttat tcgcctgctg gacgaagccc
      421 tgatcacgcc acttgatgcc agccgcacac aactggtgtg gatcactgcc gatggtaatc
      481 ctctgaaatt cgtcatgcgc agcgagctgg gcgacggtcc gcttgcctta ctgaagctac
      541 aggggtttac gctgcccacc gccattttta acgttgccgc cggcacggaa ggtgttgagt
      601 aaaaatgaac aggggcattt atcc
//
LOCUS       DAZGHF010000456          621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGHF010000456 DAZGHF010000000
VERSION     DAZGHF010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.460"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..347)
                     /locus_tag="ACHK3A_005368"
     CDS             complement(<1..347)
                     /locus_tag="ACHK3A_005368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743278.1"
                     /translation="MNKWYDEINNIADRIDKEAVAHAIEITREYISNINDISYELEKG
                     CGYYIIKNVPVDDYMPEPPSNGLRPYNKRYVSELSLAGITGALGYNVFSYAQEKKGAF
                     FHDITPVSGKEET"
BASE COUNT          209 a          130 c           63 g          219 t
ORIGIN      
        1 cttgtctcct ctttaccgct aactggggtt atatcatgga aaaatgcccc tttcttctct
       61 tgagcataag agaatacatt atatcctaat gcccctgtaa tccctgcaag agatagttcg
      121 ctgacatatc ttttattata tggtcgtaat ccattgctgg gaggttcagg catataatca
      181 tcaacaggaa cattttttat aatgtaataa ccacaccctt tctctaattc ataagaaata
      241 tcatttatat tactaatata ctccctggta atctcaatgg catgagcaac agcttcctta
      301 tcaatacgat cggctatatt attaatttca tcataccatt tattcatgtt cactctcctt
      361 taacttgata acaaacatta tttaaaaaat atttgctatc attttttatt ctttttaact
      421 caatgttttt atgcacaaca aaaatttctg cgcttaatat agataacagc aaggcgctta
      481 ttaatatact tttaaatatc attaacacac ctcacaaaac aacaatccca cattcttctt
      541 acaacacaaa aaacaaaaaa ttaacacact gtgattgttt tattgaactt catcacactt
      601 taattattca catcacgata a
//
LOCUS       DAZGHF010000457          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGHF010000457 DAZGHF010000000
VERSION     DAZGHF010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.461"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(309..>608)
                     /locus_tag="ACHK3A_005369"
     CDS             complement(309..>608)
                     /locus_tag="ACHK3A_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743279.1"
                     /translation="LEQVVTWDSDNIFTPNIFEHNGRCFMKKNETWMLLNGDATPNIR
                     TMSNKKIECLEKSEVEWASRVCDSITDKERENLKALICSKPAKSVNTGKRPALGY"
BASE COUNT          154 a          123 c          106 g          225 t
ORIGIN      
        1 tatgctccat caatttttta agttccattt tgttctcctt aattatgtaa ggagtatttg
       61 atcatgtata agcaataaaa aacagcttta ggtaatgagg aatatctcaa tttttaaaca
      121 taaaatgcga attatttagt acaaaaaagc agcgttgcgt gatctttccc tagtccctgt
      181 cccggattgt agtaccggtg ccagttgtag tgcagttcgg attcgtcgtc atactgctag
      241 cccggatggc ggagcagttg ctctagtcac tcagaatatt gtaatagtga tagagtgcag
      301 tgccggtttc aatatcctaa cgctggtctt ttgcctgtat taactgattt agctggtttg
      361 ctacatatta atgccttcag attttccctt tccttgtctg tgatactgtc gcaaacccta
      421 ctagcccatt ctacttcact cttctccagg cattctattt tcttattgct cattgttcta
      481 atgtttggtg ttgcatctcc atttaataac atccatgttt catttttctt cataaaacat
      541 cttccattat gttcaaagat attaggtgtg aatatattat cagaatccca tgttacgact
      601 tgctcgag
//
LOCUS       DAZGHF010000458          583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGHF010000458 DAZGHF010000000
VERSION     DAZGHF010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.462"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>583)
                     /locus_tag="ACHK3A_005370"
     CDS             complement(<1..>583)
                     /locus_tag="ACHK3A_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO8743280.1"
                     /translation="FSITFIPAFLTNRQELISRGLLVPALFLAEFLILVPLYFIFFRK
                     REGLGRGSFRLDVFFTLFSLIMIIQLFVPYLLAVRKTEEWMISQMSFSGAILWINIFS
                     SVLLVPVYEEIVFRGCLFNSFKFWFNDNIYISAIVTSVIFSALHLQYTDFRTFLMLFL
                     ISLVLISAKIKSNGLLMPILLHMAMNAVITGIQ"
BASE COUNT          234 a           95 c          103 g          151 t
ORIGIN      
        1 tattgtattc ctgttatgac tgcattcatc gccatgtgaa gtaatatggg cattaacagg
       61 ccattcgact ttatttttgc actgataaga actaacgata tcagaaagag catcaaaaat
      121 gtacgaaagt cagtatattg tagatgtaat gcagaaaata tgactgatgt tacaatagca
      181 gagatgtaaa tattgtcatt aaaccagaat ttaaaagagt taaaaagaca ccctcgaaat
      241 acaatctctt catatacagg aacaagaagt acggaagaaa atatattaat ccataaaatg
      301 gccccggaaa aagacatttg cgagatcatc cattcttccg ttttcctcac tgccagcaga
      361 taagggacaa ataactgaat aatcattatt aaagagaaaa gagtaaaaaa aacatccagt
      421 cgaaaagaac ctcttcctaa tccttctctt tttcggaaga aaataaaata cagtggaaca
      481 agaattagaa attcagcaag aaataatgct ggaacaagaa gtcccctgga tataagttcc
      541 tgcctgtttg taaggaatgc aggaataaag gtaatagaaa atg
//
LOCUS       DAZGHF010000459          578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGHF010000459 DAZGHF010000000
VERSION     DAZGHF010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.463"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..352
                     /locus_tag="ACHK3A_005371"
     CDS             <1..352
                     /locus_tag="ACHK3A_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8743281.1"
                     /translation="KPLVSSPFIIRNIAVSNGQYVNSGDDIVDIELLDKFFIDVKIDP
                     VSEQGNIKEKKIRYHSLVNGLSGLASVVRITRTFENGQDDKSSGLRVVTLAIDGDHKK
                     LAELLDTAFEIEIK"
     gene            349..>578
                     /locus_tag="ACHK3A_005372"
     CDS             349..>578
                     /locus_tag="ACHK3A_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO8743282.1"
                     /translation="MIKLDIYEKQFKDKKILSSISVNVEKGSFHVITGPSGVGKSSLL
                     NILGLLDSEFDGNYEFCGKNINPKDYNKTSRI"
BASE COUNT          193 a           78 c          119 g          188 t
ORIGIN      
        1 aaaaccgttg gtatcatcgc cttttatcat aaggaatatt gctgtaagca atgggcaata
       61 tgtcaattca ggagacgata tcgtagatat tgaacttctg gataagtttt tcattgatgt
      121 taaaattgat cctgtgtctg aacaaggaaa tatcaaagaa aaaaagataa gatatcactc
      181 tttggtcaat ggattatccg gtttagcatc tgtagtccgc atcacccgta ccttcgaaaa
      241 tggccaggat gataaaagtt ctggtttgag ggttgtaaca ttggccattg atggtgatca
      301 taaaaaactc gctgaattac tcgatactgc ttttgagatt gaaataaagt gattaaatta
      361 gatatttatg aaaaacagtt caaggataaa aaaatcctta gttctatatc tgttaatgtt
      421 gaaaaaggtt catttcatgt tattacaggg ccttccgggg tcggaaagag ttcactgtta
      481 aacatacttg gtttgttgga tagtgaattt gatgggaatt atgagttttg tggtaaaaat
      541 attaatccga aggattacaa taaaacttct cgtataag
//
LOCUS       DAZGHF010000460          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGHF010000460 DAZGHF010000000
VERSION     DAZGHF010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.464"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(95..>568)
                     /locus_tag="ACHK3A_005373"
     CDS             complement(95..>568)
                     /locus_tag="ACHK3A_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743283.1"
                     /translation="AVWQWQGFNFTWQADQIRELMQRFNITYIGIDITGIGKGVFDIV
                     SRFAPREANAILYSVESKNRLVMKMIDVVAHKRIEWAKDAIDEASRERTEIPASFMSI
                     RRTTTKSGNALTFVAERSDVTGHADVFFAISHAVINEPVDYDYERPSTWAFGKAA"
BASE COUNT          115 a          163 c          146 g          144 t
ORIGIN      
        1 accggttccg gctgaccaaa cgtgattgtg ctgccacgtg cgggcgtgaa tgcttttttc
       61 gtcacgcgtg acgttttgtt tctgcgtttc ttgctcatgc ggctttcccg aaggcccatg
      121 tggacgggcg ttcatagtca taatctacag gctcattaat cacggcgtgc gaaatggcaa
      181 aaaacacatc cgcatgcccc gtaacatccg accgttccgc cacgaacgtc agcgcgttac
      241 cgctttttgt ggtggtgcgg cgtatggaca tgaacgacgc ggggatctcc gtgcgttcac
      301 ggctggcctc atcaatggcg tctttcgccc actcgatacg cttatgggcc accacgtcta
      361 tcattttcat caccaggcgg tttttgctct ccacgctgta aagaatggcg ttagcctcac
      421 gtggcgcaaa gcgactgacg atatcaaaca cccctttccc gataccggtg atatctatgc
      481 cgatataggt aatgttaaag cgctgcatca gttcgcggat ctgatcggcc tgccaggtga
      541 agttaaatcc ctgccactgc cagacggc
//
LOCUS       DAZGHF010000461          566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZGHF010000461 DAZGHF010000000
VERSION     DAZGHF010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.465"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            223..>566
                     /locus_tag="ACHK3A_005374"
     CDS             223..>566
                     /locus_tag="ACHK3A_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743284.1"
                     /translation="MAGSRASGDVTIIMIAENTHDLFIQSMAALTAVLPVPVFTQAQR
                     MAQAAASLSTDKMQIPITTDTLPAPVHLSVATMRSAVSAARAAQARQLAAQRPDAAGL
                     RRQISRFISRRAG"
BASE COUNT          128 a          185 c          154 g           99 t
ORIGIN      
        1 gccggatatt gtctggcctg aatccccttc gccggatcat cacgcaggct gaccggaggc
       61 accatgacgt ggacaagaaa aacaatcagc gtcagcgctg acatgccccc gctgagctgt
      121 tccgtcatcc ccgtcacacc gtggacttac gggctgggac gatatgaaga atcgggggta
      181 tatttaagcc ctccgaacgc cataaactgg ctggccggga aaatggccgg aagccgtgca
      241 tccggcgacg tgaccattat catgattgcc gaaaacaccc acgatctttt tatacagtca
      301 atggcggcac tgaccgccgt tctgcccgtg ccggtattca cgcaggcaca gcgcatggca
      361 caggctgccg ccagtctgag cactgacaaa atgcaaatcc cgatcaccac cgacaccctg
      421 cccgcacctg tgcatctttc cgttgccaca atgcgcagcg cagtcagtgc cgcccgtgca
      481 gcacaggcca ggcagctggc agcacaacgc ccggatgccg cagggcttcg ccgccagata
      541 agccgcttta tcagcagacg ggctgg
//
LOCUS       DAZGHF010000462          563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGHF010000462 DAZGHF010000000
VERSION     DAZGHF010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.466"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>563
                     /locus_tag="ACHK3A_005375"
     CDS             <1..>563
                     /locus_tag="ACHK3A_005375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO8743285.1"
                     /translation="KYITGNNLNLIYDDVHIQNKPMLNYANSARLRKFIIIGTLCEGK
                     GQKIAIEAMHNLIREGYLCHLKIIGNNRVPYASYLNKIVADYNLSDYVEFMGFRHDLD
                     QIRLDNDVCLIPSLSEAFGRVTIESMAAGMIVVASDSGASKEIINDGINGFLFSSGSV
                     SDLTSVLKKILDVESNNLECIRKRALV"
BASE COUNT          183 a           70 c          117 g          193 t
ORIGIN      
        1 aaatacatta caggaaataa tcttaatcta atatatgatg atgtccatat ccaaaacaaa
       61 ccaatgctaa attatgctaa ttcggctagg ctgcggaagt tcattattat tggcacattg
      121 tgtgagggca aaggtcaaaa aatagccata gaggctatgc ataatctgat tcgtgaagga
      181 tatttatgtc acttaaaaat aattggaaat aatagagttc catatgcaag ttatcttaat
      241 aaaattgttg ctgattataa tttgagtgat tatgtagagt ttatgggatt taggcatgat
      301 cttgatcaaa taaggcttga caacgatgtt tgcttaattc cctctctttc tgaagctttt
      361 ggtagagtta ccattgaatc aatggctgca ggtatgatag ttgttgctag tgattctggg
      421 gctagtaaag agatcatcaa tgatggtata aatggttttt tgttttcttc aggttcggta
      481 agtgatctta ctagcgtact taaaaaaatc cttgatgtag agtctaataa tttagaatgt
      541 ataaggaaaa gggctttggt tga
//
LOCUS       DAZGHF010000463          563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGHF010000463 DAZGHF010000000
VERSION     DAZGHF010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.467"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..540
                     /locus_tag="ACHK3A_005376"
     rRNA            <1..540
                     /locus_tag="ACHK3A_005376"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          139 a          134 c          167 g          123 t
ORIGIN      
        1 gaactttcca gagatggatt ggtgccttcg ggaactgtga gacaggtgct gcatggctgt
       61 cgtcagctcg tgttgtgaaa tgttgggtta agtcccgcaa cgagcgcaac ccttatcctt
      121 tgttgccagc ggtccggccg ggaactcaaa ggagactgcc agtgataaac tggaggaagg
      181 tggggatgac gtcaagtcat catggccctt acgaccaggg ctacacacgt gctacaatgg
      241 cgcatacaaa gagaagcgac ctcgcgagag caagcggacc tcataaagtg cgtcgtagtc
      301 cggattggag tctgcaactc gactccatga agtcggaatc gctagtaatc gtggatcaga
      361 atgccacggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc atgggagtgg
      421 gttgcaaaag aagtaggtag cttaaccttc gggagggcgc ttaccacttt gtgattcatg
      481 actggggtga agtcgtaaca aggtaaccgt aggggaacct gcggttggat cacctcctta
      541 ccttaaagaa gcgtactttg cag
//
LOCUS       DAZGHF010000464          562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGHF010000464 DAZGHF010000000
VERSION     DAZGHF010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.468"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..416)
                     /locus_tag="ACHK3A_005377"
     CDS             complement(<1..416)
                     /locus_tag="ACHK3A_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743286.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPTQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILLR
                     GKETETISERLIREADTREYELWRPVRRFFSIIFLIV"
BASE COUNT          188 a           97 c          101 g          176 t
ORIGIN      
        1 acaatgagaa aaataatgga gaaaaagcga cggaccggac gccatagttc atattctcgt
       61 gtatcagcct cacgtatcaa cctttctgaa atggtttcgg tttcttttcc tcgcagcaga
      121 atatgtctgt aatatttcct catgacatat accgaaaact gaaaccagat taatatggat
      181 ggacacacaa cacataaact tctgatgata tcacccggga aagtatcttg ggtgggtaaa
      241 agaaagtcat atactctgga taaaatcatc atcgagataa atgagacgcc agcacctatc
      301 cagtattcga tatgagaaga tgctgcatta tatgcgtcag taaaattttt ctttcttaat
      361 gaaggtggaa gtgctgaaag ttcaggaatg cgttttcttg tccagtatat tttcatatag
      421 taactcatat aaaaagtgtt ctactttact atgatgatga cgatttataa aaggcttata
      481 atgactactt ctcctttcat tctcgaattt cacgataata atagagataa tcattatcaa
      541 ttgatagtgt cagtaatttt ga
//
LOCUS       DAZGHF010000465          560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZGHF010000465 DAZGHF010000000
VERSION     DAZGHF010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.469"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..350)
                     /locus_tag="ACHK3A_005378"
     CDS             complement(<1..350)
                     /locus_tag="ACHK3A_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743287.1"
                     /translation="MRDKTREAMRFFLGGRCYTAENLEKDYLAEVANYSDDRWEAPQR
                     AARLAAAVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRSGSQWLVVEVF
                     GEKGRQHRSADSNP"
BASE COUNT          143 a          148 c          124 g          145 t
ORIGIN      
        1 tccgggttac tgtccgcgct gcggtgctga cgccccttct caccaaaaac ttcaacgacc
       61 agccactggc ttccgcttct gccgaacaat gccttacaca aacgtctcac ggtcagcggg
      121 gttaaatctg gatcgggatc atatgcaatg gtagcaaaaa taaagcgcag catttctgat
      181 gtcttgtaac gctttacggc agctgccaga cgtgcggcgc gttgaggtgc ttcccagcgg
      241 tcgtcactgt aattagcaac ttcagccaga taatcctttt caaggttttc agccgtgtaa
      301 cacctgccgc ctaaaaaaaa ccgcatggcc tcccgggttt tgtctctcat atatcatccc
      361 tcattaatgc taatgtatgc atacattagc attaatatct gacagcaaca agcgacggat
      421 aattgccaga attgcagggc tggagataca tttggataac gtaatgatcg cagtatacag
      481 gctaggctgt caggttttca gctgtatctg ttcctgcagc agctccagat gatatacagc
      541 aatagcattt accacccctg
//
LOCUS       DAZGHF010000466          543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGHF010000466 DAZGHF010000000
VERSION     DAZGHF010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.470"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..539)
                     /locus_tag="ACHK3A_005379"
     CDS             complement(<1..539)
                     /locus_tag="ACHK3A_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFO8743288.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGHWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMP"
BASE COUNT          127 a          177 c          125 g          114 t
ORIGIN      
        1 ggcatcttcc tgaaccggaa ccggtatacg ccctccggta cagatatccc cggctgccag
       61 catctgccgt cggcctgttc gccttccagt tccgccagca acggcaggtg ataaaacaga
      121 gccgaccaga acaccaccat gttccacaca ctcccctgcg cggcctgctc ttctgctgtc
      181 gctcccggtg gaaacattct gcctctggcc agccggacag catacgtcgt gaactgcagg
      241 gtcaggtcac caaaaccacc ggtgtaaccc cagtgacctt ctgctgtggc cggcagattc
      301 tgactgacag acaacaactg atgcacagga tcaagacaaa ggcgctgata catatccacc
      361 ggcatggaac tgttttccca taactgtttc agacaccttt tcctgaacgg tgtggatacc
      421 agtacatcag ccggcaatgg tgtaaaatac ccctccggca ccgtttcccc gggaatatcc
      481 gtccggacat cagagcattc tgtcttcaca ggaaataacc ttttcaggcg ttcaagcatg
      541 ccc
//
LOCUS       DAZGHF010000467          538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZGHF010000467 DAZGHF010000000
VERSION     DAZGHF010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.471"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT          173 a           98 c           69 g          198 t
ORIGIN      
        1 atcaaaccga ccagataaga aacgcaacta ttttaacttt gctaatttgt tgttcactca
       61 gaatttttag agtcattacc atcttgaatg ctaattttta ctttaacatc atcattgtta
      121 atcccaacaa ctccaataca gtaatctatt gacttatgca cctgaatttt attttcggaa
      181 tccatagaga taattctttt tgctccaacc tcgcaagtat ttcccatggg gaatggaata
      241 ttcaaacttc tgtccaagag taagcaagga gagaataaga tatcaaacag gaaggatatt
      301 catactcaca cctatgcttc aaaacaactt tatcattaaa agaggtttca cattgtgttc
      361 catttttatt gaatgaactg taaaaaatcg aagctgttgt ctatgctata cataggtaaa
      421 agtgcaacta tcaaagatag agtttttctc atgttaactt ctatatcttt cttttattcc
      481 taattttagc ctttctctta ctttaatact tgcttcatat tttgttattg cattatat
//
LOCUS       DAZGHF010000468          537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZGHF010000468 DAZGHF010000000
VERSION     DAZGHF010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.472"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>537
                     /locus_tag="ACHK3A_005380"
     CDS             <1..>537
                     /locus_tag="ACHK3A_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8743289.1"
                     /translation="VSSEEASGYLAAMGARFRSEFSAMGAVGFAEDLAGKTAWMVQQF
                     GVEMSTMQELIEGTKNAGADFGVSMAEQFAILGTLSRTMGTEASGIYEEFLRSAPAAA
                     QKMGMSFVDATGKILPMGDILDRLQARFGRNIEGNVAAQQALDDAFGSGADVIKKLWG
                     QQEMLNRSISALGRNDGMK"
BASE COUNT          120 a          131 c          182 g          104 t
ORIGIN      
        1 gtcagcagcg aagaggcgag cggctacctt gccgccatgg gtgcccgttt ccgcagtgag
       61 ttttctgcca tgggtgccgt ggggtttgct gaagatctgg caggtaaaac cgcgtggatg
      121 gttcagcagt tcggcgtgga aatgagcacc atgcaggagc ttattgaggg caccaaaaac
      181 gcgggcgcgg attttggcgt cagcatggca gagcagtttg ccatactggg caccctgtca
      241 cggaccatgg gcaccgaggc atcgggcatc tatgaggaat tcctgcgcag tgcccccgct
      301 gccgcacaaa aaatggggat gagttttgtt gatgccaccg ggaagatttt acccatgggt
      361 gacatcctcg accgtctgca ggcgaggttc ggcaggaaca tcgagggtaa cgtggcggca
      421 caacaggcgc tggatgacgc cttcggcagt ggcgcggacg tgattaaaaa actctgggga
      481 cagcaggaaa tgcttaaccg gagcatatca gcgctggggc gtaatgacgg catgaag
//
LOCUS       DAZGHF010000469          533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGHF010000469 DAZGHF010000000
VERSION     DAZGHF010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.473"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..185)
                     /locus_tag="ACHK3A_005381"
     CDS             complement(<1..185)
                     /locus_tag="ACHK3A_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO8743290.1"
                     /translation="MKTSDNESTKYAITGQAVLQILRMKINFSLQTLIKQLLVMKSVE
                     ENAFRRDLIDSIIRDFS"
     gene            complement(228..464)
                     /gene="ycgZ"
                     /locus_tag="ACHK3A_005382"
     CDS             complement(228..464)
                     /gene="ycgZ"
                     /locus_tag="ACHK3A_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO8743291.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRIL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          159 a          112 c           93 g          169 t
ORIGIN      
        1 ttgctaaaat cacgaattat gctgtcaatt aaatctcgtc ggaaagcatt ttcctcaaca
       61 gatttcatta cgagtaattg cttaataaga gtctgcaacg aaaaatttat cttcatgcgc
      121 agtatctgga gcaccgcctg gccagtaatt gcatattttg tactttcatt atcagatgtc
      181 ttcataacga cttcatagct aacctgttaa ataatcaggc tgttgcatta ttcaaaaagc
      241 aacccaatta gtgcattata atgtttctgt tcctcttccc ctgtcgcttg ttccagtcgg
      301 catagaagtt tggcacaaag cgactttcga ctcagaatac gtccatcttt caaaatctca
      361 gtcacaattt ccccgagagt ttcctgctga gtaggcaagt tagcttttgc aaagtaacga
      421 gtgattgctc ccgcagaatc taaagtcact gaattttgat gcatgctacg cctctgttaa
      481 aaatgttaat caatgttaag tgaggtaaca atagcaaact tgtacgaaat atg
//
LOCUS       DAZGHF010000470          532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGHF010000470 DAZGHF010000000
VERSION     DAZGHF010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.474"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            69..305
                     /locus_tag="ACHK3A_005383"
     CDS             69..305
                     /locus_tag="ACHK3A_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO8743292.1"
                     /translation="MSQYPELIAQFSSGNQTRIKQGLTAKAPLEGWRYGPKEIVKKFQ
                     IHHRVAIEYGGSVYDIDNLRIVTPRLHDEIHYRR"
     gene            308..>532
                     /locus_tag="ACHK3A_005384"
     CDS             308..>532
                     /locus_tag="ACHK3A_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO8743293.1"
                     /translation="MFKEKLEDYTEEEFLNFLGGLRSTMKDGKPLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPENIL"
BASE COUNT          186 a           92 c          111 g          143 t
ORIGIN      
        1 aaaacaggta aaaaatactg aaatcatttt atcaatttct cgcgttcacc gatacccttt
       61 ggctggaggt gagtcagtat ccggagttga tagcgcagtt ttcaagtggt aaccaaacac
      121 gtataaaaca aggattaacc gcaaaagcac ctcttgaagg ttggcgttat ggccctaaag
      181 aaatcgttaa aaaattccag atacatcatc gtgttgcaat tgaatatggc ggcagcgtat
      241 acgatattga taatttacga attgttaccc ccagactaca cgatgagatt cactacagga
      301 gataaatatg tttaaagaaa agctcgaaga ttataccgag gaggaatttc ttaatttttt
      361 aggcggattg agatcaacta tgaaagatgg aaagccctta aaaggtaagg agctagaaat
      421 gtactgggat tcattagtag atcattttat tgaaattaca cagcatccgt cagggagcga
      481 tctaattttc taccctaaaa gtcaaggaga tgataagcct gaaaacatcc tg
//
LOCUS       DAZGHF010000471          532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGHF010000471 DAZGHF010000000
VERSION     DAZGHF010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.475"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            328..525
                     /locus_tag="ACHK3A_005385"
     CDS             328..525
                     /locus_tag="ACHK3A_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743294.1"
                     /translation="MILSRKKIEIEVVQSGTADNPETVTKIKVSRPEDLEACGVIVEK
                     ICAIHAKSVNTTASNDHNKYN"
BASE COUNT          151 a          116 c          125 g          140 t
ORIGIN      
        1 agacggtcaa caacatggtt aagcataaaa tgctcacgat gatcgttttt tgcggcatag
       61 cgcttgactc cttttacagg gaggcgctaa ccttccactt gtcacggtag gaagtgttag
      121 ggcctcgggt taacatttaa gttgactcgg ggcctttcca catccggcct tcaggtaatc
      181 cttccagcca tcagccgaaa ggcacccgcg tgtaatgtac tgatttccct ttgtgtgcgc
      241 aatgcctggc gttgaagcag acaacgctat cgcactggtg atattctttc gcacaggctt
      301 actctgtcct gtgtcggggc gatgaaaatg attctttcaa gaaaaaaaat tgaaattgag
      361 gttgtgcaaa gtggaacggc tgataatcct gagacggtta caaaaataaa ggtttcacgc
      421 ccggaagatc ttgaagcgtg tggtgttatc gtggaaaaaa tttgtgctat tcatgcaaaa
      481 tcagttaata ccactgccag taacgaccac aataaataca actgaacaaa aa
//
LOCUS       DAZGHF010000472          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGHF010000472 DAZGHF010000000
VERSION     DAZGHF010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.476"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>528
                     /locus_tag="ACHK3A_005386"
     CDS             <1..>528
                     /locus_tag="ACHK3A_005386"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743295.1"
                     /translation="PVSVMAKAQKGSVINSITFTDSLTDETDDKHDFTNTGDLKGSTP
                     SIMTFPYGESDDRYTTNYVGNVQSSKTKFINLATGLTNDTTPTILISLDKELNNNQHI
                     EITRYKVDVDNDNILYEVVDEIIPSEHVDIKGKNIIVKDQLEHTYSQYYKYEFVIKDN
                     VDGKESVTSEKEFYFL"
BASE COUNT          193 a           74 c           98 g          163 t
ORIGIN      
        1 cccagtcagt gtgatggcaa aagcacagaa aggtagcgtc attaattcca ttaccttcac
       61 tgatagttta actgatgaaa ccgatgataa acatgatttt actaatacag gtgatctgaa
      121 gggcagtacg ccttctatca tgacatttcc gtatggtgaa tcagatgata ggtatacgac
      181 taactatgtt ggtaatgttc agtcctcaaa aacaaaattt ataaatcttg caacagggtt
      241 aacgaatgat actacgccaa ctatattaat atctcttgat aaggagctga ataataatca
      301 acatattgaa attacacgtt ataaagttga tgttgataac gataatatat tgtatgaagt
      361 agttgatgag ataatcccta gtgaacatgt ggatattaaa ggaaaaaata ttattgttaa
      421 agatcaactg gagcatacct atagtcaata ttataaatat gagtttgtca taaaagataa
      481 tgttgatggt aaagaatctg tgacttcgga gaaagagttt tacttcct
//
LOCUS       DAZGHF010000473          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGHF010000473 DAZGHF010000000
VERSION     DAZGHF010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.477"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            35..>528
                     /locus_tag="ACHK3A_005387"
     CDS             35..>528
                     /locus_tag="ACHK3A_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743296.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     IKQYYASRENFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFE
                     KEKLLASMEVINSMSAYVELLTKDMATTESQQVALLKQALDHANNAQTLLGVSHSKDL
                     AQSAA"
BASE COUNT          173 a          101 c          115 g          139 t
ORIGIN      
        1 tatctgagat aaactcaacg tcaagaggtg ggttatgatt agatatagtg gaatatttct
       61 atcgctgtta atgctttcag gttgtgcaac tgaagcattg aaaaacgaac aagctgaaac
      121 tgttattact gcgggtaaca aagcagtagc ggcgaatgca agtttcatta aacaatacta
      181 tgcttctcgt gaaaacttcc ttgttaattt ctacgcgact aatccggatt gtgccctgcg
      241 acctgattat accattgata taggcggtga tgggaatggc ggaaaattat gccagcgttc
      301 agggccaaat acaaaacaag tacaattagt ccctttcgag aaagaaaaat tactggcctc
      361 aatggaagtc attaatagca tgagcgctta tgtggaactt ctgacaaagg atatggcaac
      421 tacggaaagt cagcaagtgg cattacttaa acaggctctg gatcacgcaa acaacgcaca
      481 aacactactt ggtgtttcac atagcaaaga tttagctcaa tctgcagc
//
LOCUS       DAZGHF010000474          519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGHF010000474 DAZGHF010000000
VERSION     DAZGHF010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.478"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..206
                     /locus_tag="ACHK3A_005388"
     CDS             <1..206
                     /locus_tag="ACHK3A_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO8743297.1"
                     /translation="RAKWFRFAGLYLLRDEAGKPRATAINDTAVLEKADAYLAQAAAL
                     SRTAGVKSIRARIRARISALNVA"
     gene            314..>519
                     /locus_tag="ACHK3A_005389"
     CDS             314..>519
                     /locus_tag="ACHK3A_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO8743298.1"
                     /translation="MSEPSFSVSGKPLKSTPQTVTNGEPFWPDLDLAELQGSRTLPAD
                     LPDDTAAMALLAAVAEVNTTLAPVV"
BASE COUNT          112 a          152 c          167 g           88 t
ORIGIN      
        1 tccgcgcgaa gtggttcaga tttgccgggc tgtatcttct gcgcgacgag gcgggcaaac
       61 cgagagccac cgcgattaat gatactgccg tactggaaaa ggcagacgca tacctggcac
      121 aggcggcagc actgagccgt accgctggcg taaaaagtat cagggcgcgc atcagggcac
      181 gcatcagcgc ccttaatgta gcgtaaacga ctacccgcag ggccgggcgg gcgcgggagg
      241 aggcatcagt gacggaaact gtatggccgt ggaatcccgg acagcccgcc tttttattca
      301 tatgaggcac gtcatgagcg aaccgagctt cagcgtcagc ggtaaaccgc tgaaatccac
      361 accgcagacg gtgacaaacg gcgagccgtt ctggccggac cttgatctgg ctgagcttca
      421 ggggtcacgc acgcttccgg ctgatttacc cgacgacacg gcggccatgg cgctgcttgc
      481 cgcagtggcc gaggtgaaca ccacgctggc gccggttgt
//
LOCUS       DAZGHF010000475          518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGHF010000475 DAZGHF010000000
VERSION     DAZGHF010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.479"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..346
                     /locus_tag="ACHK3A_005390"
     CDS             <1..346
                     /locus_tag="ACHK3A_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm52 family immunity protein"
                     /protein_id="HFO8743299.1"
                     /translation="NTSDMIDSLLLLAEENNNSWIKVDSKGYWRNDSNVFPDRVYVGW
                     MLYMPHILLPELIPEAAKIVPVFEGEKQKGTIVVSTEDVFDGNNKEHIGKANDIEIRL
                     LDLGLLPLLTEL"
BASE COUNT          172 a           87 c          106 g          153 t
ORIGIN      
        1 caatacttcg gatatgattg attccttgct tctgttggct gaagaaaata ataattcatg
       61 gataaaagtc gactcaaaag gttattggcg taatgacagt aatgtttttc ctgacagggt
      121 atatgtggga tggatgttat atatgcccca catactctta cctgaattaa tccctgaagc
      181 cgcaaaaata gtaccggttt tcgagggcga gaagcaaaag ggaactattg ttgtttcaac
      241 cgaagacgta tttgacggaa ataataagga acacatcgga aaagctaatg atattgaaat
      301 aagacttctg gatcttggac tgctaccttt gctaacagag ctttaatcca accttatcac
      361 tcatgggtag atgacgggac agttacaccc gttaatggcc ttatttttaa cacatcagat
      421 aaaattgaat gctcaccatt aagaatacct ggagaaatta aaaaacggag attgtttcaa
      481 gagcttcttt tatttaaggg tttacaggtc aaaaatgt
//
LOCUS       DAZGHF010000476          514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGHF010000476 DAZGHF010000000
VERSION     DAZGHF010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.480"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>514)
                     /locus_tag="ACHK3A_005391"
     CDS             complement(<1..>514)
                     /locus_tag="ACHK3A_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO8743300.1"
                     /translation="QNQSVPEVVEQVLRKHGLEGADFEFRLERAYPSREIITQWQETD
                     LQFIQRILSEVGIFWRTEMDDVRGLDTYIFADSQLNYQFDVCLPYSEPSGLFDGAAES
                     VWDVRTWHNVATGTVSTRDYNYRTASMPMDATVSVRNDAVTTGEHYRDAPAYREAGDD
                     TSPEPETESGA"
BASE COUNT          111 a          163 c          134 g          106 t
ORIGIN      
        1 gcaccagatt cggtttccgg ctccgggctg gtgtcatccc ccgcttcacg ataagccggt
       61 gcatcgcggt aatgctcccc ggtggtgacc gcatcattac gcacgctgac cgtcgcatcc
      121 atcggcatgg aggcagtccg gtagttgtaa tcacgcgtgg agaccgtgcc ggtggcgacg
      181 ttgtgccatg tccgtacatc ccagaccgat tctgcagcac cgtcaaaaag gcccgacggc
      241 tcgctgtacg gcagacacac atcaaactgg tagttaagct ggctgtcggc aaaaatgtac
      301 gtatccagtc cgcgcacatc atccatctcc gtgcgccaga aaatccccac ctcagacaga
      361 atgcgctgaa tgaactgcaa atccgtttcc tgccactggg tgataatttc acgcgacggg
      421 taagcgcgtt ccagtctgaa ttcaaaatca gcgccctcca gcccgtgttt gcgcagcacc
      481 tgctccacca cctcagggac ggactggttc tgaa
//
LOCUS       DAZGHF010000477          508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGHF010000477 DAZGHF010000000
VERSION     DAZGHF010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.481"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>508)
                     /locus_tag="ACHK3A_005392"
     CDS             complement(<1..>508)
                     /locus_tag="ACHK3A_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743301.1"
                     /translation="KHAYAAGIRPELLGGVAWIESGGMPENYKFQIYETKRMIGSLDM
                     PENKTSFGSMGIQIRTAAITLGLDPSELTTRNQLELATCLMEDDFTFKIAATHLRDLA
                     LFDYPSSATLYMTNEQYIMAGIRYNRGVERDLGFFIYLINNLPARDTDDYKFISYGMR
                     LLEIREHIK"
BASE COUNT          160 a          109 c           81 g          158 t
ORIGIN      
        1 ttttgatatg ttctctaatc tctaacaacc tcataccata agagatgaat ttataatcat
       61 ctgtatcacg cgcaggcaaa ttattaatta aataaataaa aaaacccaaa tctcgttcaa
      121 caccacgatt atatctaata ccagccataa tatactgctc attcgtcata tacagtgttg
      181 ccgaagaggg ataatcaaaa agggcaagat ctcttaaatg agtagcagca attttaaagg
      241 taaaatcatc ttccatcagg catgtcgcta actctaactg atttctggta gttaattctg
      301 aagggtcaag gcctagagta atcgctgcgg ttcgtatttg tatacccatt gagccaaatg
      361 atgttttatt ttcaggcata tctaacgatc cgatcattct ttttgtttca tatatctgaa
      421 acttataatt ttcaggcata cctcctgact caatccaggc cacgccgccc agtaactctg
      481 gacgaatccc tgcggcatat gcatgttt
//
LOCUS       DAZGHF010000478          501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGHF010000478 DAZGHF010000000
VERSION     DAZGHF010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.482"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..468
                     /locus_tag="ACHK3A_005393"
     CDS             <1..468
                     /locus_tag="ACHK3A_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8743302.1"
                     /translation="KGGWNFYQYPLNPVSGFDPLGLKVSFQGDESTQKTLKEAYKAVA
                     ETKFGHKITEELESSEHEYIFRGLRKGINQTCYDDTEYSFYIDIDNDHSSCVYQGKNK
                     ACAMKPTLLSVVLAHEMGHAKGMKDDGTDSMANVDKYENPFRKELGLPARMKY"
BASE COUNT          184 a           66 c          110 g          141 t
ORIGIN      
        1 aaggggggat ggaattttta tcagtatccg ttgaatccgg tatctggttt cgatccatta
       61 gggttaaaag tgtcttttca gggagatgaa agtacacaaa aaactttaaa agaagcatat
      121 aaagctgtag cagaaacaaa gtttggacat aaaataacag aagaattaga gtcatcagag
      181 catgaatata tatttagagg attaagaaaa ggcataaacc aaacatgcta cgatgatact
      241 gaatatagtt tttacattga tattgataac gaccatagct catgtgtata tcaaggaaaa
      301 aacaaagcat gtgccatgaa acccactctg ttgagtgtgg ttcttgcgca tgaaatgggg
      361 catgcaaagg gaatgaaaga cgatggaact gactcgatgg ctaatgttga taaatatgaa
      421 aacccatttc gtaaagagtt gggcttgcca gcaagaatga aatattaaac atttttatat
      481 tgcttttggg tatgttttta a
//
LOCUS       DAZGHF010000479          499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGHF010000479 DAZGHF010000000
VERSION     DAZGHF010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.483"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(45..368)
                     /locus_tag="ACHK3A_005394"
     CDS             complement(45..368)
                     /locus_tag="ACHK3A_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO8743303.1"
                     /translation="MKWIVIDTLIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGESEIPNKCLHNSCIIKIC
                     PYGLK"
BASE COUNT          163 a           80 c           78 g          178 t
ORIGIN      
        1 tggatattat taatagcatg tcgagcatat ttatatgaag cccattactt gagtccatat
       61 gggcatattt ttataataca actattatgc aaacatttat ttggtatttc gctttctcca
      121 ggaggacact cttgactgct tttgagtaaa ctccataaat ccttgttaaa tggtgcgatg
      181 ttataaatag taatagggta ttctttatcc ttaaggataa taccagactt aaccggtgta
      241 aatatacttc caggagggag aaatatagta gattgatacc agatgatcat tttcatatca
      301 ccccatatgg ctgaaaaaga tatgccgcat gtaggttgaa ttaacgtgtc aattactatc
      361 cacttcattt gttatgtctt atcccacggt atttaatata gttcattagg atgttcattt
      421 cttgattttg catatgagta tattatcccc tcaaaaatat taattaaaaa ttaaaaccgt
      481 tgcctatata aaacaaaat
//
LOCUS       DAZGHF010000480          496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGHF010000480 DAZGHF010000000
VERSION     DAZGHF010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.484"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>496
                     /locus_tag="ACHK3A_005395"
     CDS             <1..>496
                     /locus_tag="ACHK3A_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO8743304.1"
                     /translation="MRDDVAFQIINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI
                     NKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMK
                     WRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIE
                     ACDE"
BASE COUNT          132 a          119 c          133 g          112 t
ORIGIN      
        1 aaatgcgcga tgatgttgca tttcagatta tcaatgatga attatatctt gatggcaacg
       61 ctcgtcagaa cctggccact ttctgccaga cctgggacga cgaaaacgtc cataaattga
      121 tggatttgtc gatcaataaa aactggatcg acaaagaaga atatccgcaa tccgctgcaa
      181 tcgacctgcg ttgcgtaaac atggttgccg atctgtggca tgcgcctgca ccgaaaaatg
      241 gtcaggccgt tggcaccaac accattggtt cttccgaggc ctgtatgctc ggcgggatgg
      301 cgatgaaatg gcgttggcgc aagcgtatgg aagccgcagg caaaccaacg gataaaccaa
      361 acctggtgtg cggtccggta caaatctgct ggcataaatt cgcccgctac tgggatgtgg
      421 agttgcgtga gatccctatg cgccccggtc agttgtttat ggacccgaaa cgcatgattg
      481 aagcctgcga cgaaaa
//
LOCUS       DAZGHF010000481          493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGHF010000481 DAZGHF010000000
VERSION     DAZGHF010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.485"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            209..>493
                     /locus_tag="ACHK3A_005396"
     CDS             209..>493
                     /locus_tag="ACHK3A_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743305.1"
                     /translation="MSEIKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIR"
BASE COUNT          154 a          117 c          101 g          121 t
ORIGIN      
        1 tttccgtaat ctgatgcatc aggggtatgc ccaccacaaa taactctgta cagcaacaga
       61 aaaatttccc tggtcatatg atgaattaat catcgccatc acaccggatg cttctcatcc
      121 tgtcctcaga aaggcaggtg gtctcctgtc tgccgcgctt atccaggata ccgggacctg
      181 aaaggtcata atctggcagg aaaaaataat gtccgaaata aaaaaacacg tttcttattc
      241 attaacagtt gcagctatgc tgtcagccgc catcgtctgt cagacagcac atgctgatgt
      301 caacacaccg aacatcaccg gagaaaaaaa caatacaaca acacgattct ccggaaatat
      361 ttacggcagt gaaaatactg tcacatcaga gtcaaacagt ctggttgtgg gtaacagtaa
      421 ttccgtggag ggagggtata acaacattgt tattggtaat tcctccacgg tcaaagcttc
      481 cggtttgatc aga
//
LOCUS       DAZGHF010000482          492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGHF010000482 DAZGHF010000000
VERSION     DAZGHF010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.486"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>492
                     /locus_tag="ACHK3A_005397"
     CDS             <1..>492
                     /locus_tag="ACHK3A_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8743306.1"
                     /translation="DITASAREGKIDPVSGRDTEIRQMVDILSRRRKNNPILVGDPGV
                     GKTALVEGLALRIAEGNVPESLRPVTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQQ
                     SPAPVLLFIDEAHTLIGAGNQAGGADAANLLKPALARGELRTIAATTWSEYKQYFERD
                     AAL"
BASE COUNT          103 a          140 c          158 g           91 t
ORIGIN      
        1 gggacatcac ggccagcgcg agagagggga aaattgaccc ggtatcggga cgcgatacgg
       61 aaatccgtca gatggtggat attctctccc gccgccgcaa gaacaacccg attctggtgg
      121 gggaccccgg tgtggggaaa acggctctgg tggaagggct cgccctgcgt attgcggaag
      181 gtaacgtacc ggaatcactc agacccgtca ccctgcgcac ccttgacctc ggcctgctgc
      241 aggccggtgc cggcgtgaag ggtgaatttg aacagcgcct gaaaaacgtg attgatgccg
      301 tgcagcagtc accggctcct gtactgttgt ttatagatga agcccatacc cttatcgggg
      361 ccggtaatca ggccggcggc gcggatgcgg ccaacctgct gaagcctgcg ctggcgcggg
      421 gtgaactgcg taccattgcc gccaccacct ggtctgagta caaacaatac tttgaacgtg
      481 acgcggctct gg
//
LOCUS       DAZGHF010000483          492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZGHF010000483 DAZGHF010000000
VERSION     DAZGHF010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.487"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     repeat_region   79..412
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=79..107
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          118 a          120 c          132 g          122 t
ORIGIN      
        1 ttgtttgtgt gatactgtaa agttggtaga ttgtgactgg cttaaaaaat cattaattaa
       61 taataggtta tgtttagaga gttccccgcg ccagcgggga taaaccgaga tttcagaggc
      121 agccgccagg ccgttgatcg agttccccgc gccagcgggg ataaaccgcc atttcggtag
      181 cgccatcaaa atggttacgc gagttccccg cgccagcggg gataaaccgc gtcgttagcg
      241 gtctggtgac gttaaagggg tgagttcccc gcgccagcgg ggataaaccg ttgatcgccg
      301 tggcggtgag attagaaacg cggagttccc cgcgccagcg gggataaacc ggaagtggca
      361 aagctgccgt ttcttttctc tatgagttcc ccgcgcctgc ggggataaac caccatataa
      421 cccgttatct ctttctcaag tttttatatt agctgtactt gtaataagca acatatccac
      481 gtaacacctc at
//
LOCUS       DAZGHF010000484          487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGHF010000484 DAZGHF010000000
VERSION     DAZGHF010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.488"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>487
                     /locus_tag="ACHK3A_005398"
     CDS             <1..>487
                     /locus_tag="ACHK3A_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743307.1"
                     /translation="GNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGS
                     DTLKRRITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITET
                     RARTEGDAAALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQARTAG
                     DT"
BASE COUNT          153 a          129 c          121 g           84 t
ORIGIN      
        1 tcggtaatgc caccagaatt aacggtgatt catctgttgc cataggtctg acatcgtccg
       61 taaccgggaa cgactccgtg gcactgggca gtcattccag tgctaacgga aacaatatcg
      121 tatccgtcgg ctctgacaca ctgaaacgcc gcatcacaaa tctggcacag gggaaagatg
      181 accatgatgc ggtaaatctg atgcagatga ccagtgctgt taaccgggaa gctggagaaa
      241 gaataaaatc aacaaacaac ctgcagaatc agcttaatac cctctctggt caggtcataa
      301 ctgaaaccag ggcccgaaca gaaggtgatg cagcagcgct ggctgcagcc agagcccaca
      361 gcgatgaaaa cgacaaaaga acactggtca gtgcgaatac ctatgctgac cagaaagaaa
      421 ccgccatcaa taaccgtact gacggattac tgacgaccga acgccaggca cgcaccgcgg
      481 gcgatac
//
LOCUS       DAZGHF010000485          483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZGHF010000485 DAZGHF010000000
VERSION     DAZGHF010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.489"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            260..412
                     /locus_tag="ACHK3A_005399"
                     /pseudo
     CDS             260..412
                     /locus_tag="ACHK3A_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
BASE COUNT          118 a          105 c          114 g          146 t
ORIGIN      
        1 taattccctt gtgtagatgt acagaattgt acgatttcgg acaccaaaac cgctgttgtc
       61 ccgccattca ggcgatccct gaaagtcatt tggctaaaaa acagtcttaa cgtggtttat
      121 tttctgaatt ccaggcggac cccagtaagg gggagctgag agcgttcaat gaatagatat
      181 aatttattga ttaataaata attggctatt tttattaacc tcttaatggc gtttactgtt
      241 tcattgctcc ccaaacccct atatacaact gtcgctgtgt ggcgtggccg gagaagtagt
      301 gattggtaac tcgctctgta atgagcgacg ccgcgtactg ctgacgcccg ggtattaccc
      361 gggaaactgg gagcatcgtc tgcatcatct tcaggagcag aatgtggtat gatgcagaat
      421 gttttatcac tgcgccagtc ttcggactgg cgttttgttc aactgccagt tccctgaatg
      481 ttt
//
LOCUS       DAZGHF010000486          474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZGHF010000486 DAZGHF010000000
VERSION     DAZGHF010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.490"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            150..>474
                     /locus_tag="ACHK3A_005400"
     CDS             150..>474
                     /locus_tag="ACHK3A_005400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743308.1"
                     /translation="MAEGAMARTISQLSKGQVLSLTTSPTKENEPVFFSHGSHIHSYF
                     ICSPFFLVHEEIKNNNPELFPHGFDIIIEDTTFQMYSPDREQQIKHVKKTLKYDGIFI
                     FTEKFS"
BASE COUNT          170 a           90 c           74 g          140 t
ORIGIN      
        1 ctgactatct tacgaaaaac agtggtccat ttgttaacca ctacttttgc agtattccat
       61 acagcaaaga agaagaatgc aggctgggtg tgactattat gaaatatgca agccatcgaa
      121 ataagccatt gagcttatgg tgtcttggaa tggcagaagg agccatggcc agaacaattt
      181 ctcaactatc aaaaggacag gttttatcat taacaaccag cccaacaaaa gaaaatgaac
      241 ctgtattctt tagtcatggt tctcatattc actcttactt tatttgctcc ccattctttc
      301 ttgttcatga agaaataaaa aataacaacc ctgaactatt tcctcatggt tttgatatta
      361 taatagaaga tacaacgttt cagatgtatt ctcccgacag agaacaacaa ataaaacatg
      421 taaaaaaaac attaaaatac gatggtattt tcattttcac agagaaattt tcca
//
LOCUS       DAZGHF010000487          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZGHF010000487 DAZGHF010000000
VERSION     DAZGHF010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.491"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHK3A_005401"
     CDS             complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHK3A_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFO8743309.1"
                     /translation="TKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYFNTK
                     LENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNERTTA
                     "
BASE COUNT          112 a          128 c          108 g          125 t
ORIGIN      
        1 ttcactcact gtcagaagac cttcagacac gctaccgccc ttcgccataa cccgggcgca
       61 tcaggtctgt gttcaggatt gagaattctt tcttcagcac ccgccaacag atactgaaga
      121 aaaaagaaaa atgacggaca ggataccctg cccgtccggg agaattacgc agtggtacgt
      181 tcattccacg cgtcagaatg aatgatgtta ccgtccttat aagtccaggt gattttctcg
      241 tagcgcagct ccacctgctc aaggtggtta tgcttctcgt aggcgggatt cttgatgtcg
      301 tgcatgaccg ggttaacctt caccaccttc acgttctcaa gcttcgtatt gaaatactcc
      361 acttcctgac cggcatcatt gattctgtac cacttaaatt cagcagactt cagggtctgc
      421 cccgtggtca ccgccttgta aaggtagggg ctggaggaat caatttcctt ggt
//
LOCUS       DAZGHF010000488          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZGHF010000488 DAZGHF010000000
VERSION     DAZGHF010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.492"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>464)
                     /locus_tag="ACHK3A_005402"
     CDS             complement(<1..>464)
                     /locus_tag="ACHK3A_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743310.1"
                     /translation="NKLLLDVDNPRFPTSAENQRDAIAKMLDLQYDRIYRLAKDIASK
                     GLDPSENILVYPSEEESGFFVVAEGNRRVTALKLLHSPRLAPTEKARKAFEKLKITQL
                     KDINVIDNCVLFDDDEYEHWVNLKHTGQNAGVGRVEWTATEKARHLARMGKQ"
BASE COUNT          136 a          103 c           82 g          143 t
ORIGIN      
        1 ctgcttaccc atcctagcca gatgtcgtgc tttttctgtt gcagtccatt caactcttcc
       61 aactccagca ttttgaccag tatgctttaa attgacccaa tgctcatatt catcatcgtc
      121 aaaaagaacg cagttatcta taacattaat atctttaagc tgagtaatct taagtttttc
      181 aaaagctttc cgtgcttttt ctgttggtgc aagtctagga gagtgtaaaa gtttaagggc
      241 tgtaactcga cgattacctt cagcaacgac gaaaaaccca gattcctctt cactaggata
      301 aactaatatg ttttcagacg gatctaaacc tttcgaagca atatctttag ccaagcgata
      361 aatgcggtcg tactgcaagt caagcatctt ggcaatagca tcacgctgat tttctgctga
      421 cgtcgggaaa cgagggttat caacatccaa aagtaactta ttga
//
LOCUS       DAZGHF010000489          461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZGHF010000489 DAZGHF010000000
VERSION     DAZGHF010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.493"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>461)
                     /locus_tag="ACHK3A_005403"
     CDS             complement(<1..>461)
                     /locus_tag="ACHK3A_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8743311.1"
                     /translation="RLAALWQALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADE
                     VLPAPLPPYRVLTGLVDRFGRTQTFHREAGGEFSGEITGVTDGAGRHFRLVLTTQAQR
                     AEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPDNLP"
BASE COUNT           76 a          167 c          145 g           73 t
ORIGIN      
        1 ggcaggttgt ccggatattc cgggtcgtgc gtcagccaca cggcagacag acggatgccg
       61 ttgtcccggc catattcggt gtaacccggc agggtatcag gaaaagcgga cggttccgtc
      121 ccgccggaaa tggcctgctg ccgggcctct tctgcccgct gcgcctgcgt ggtcagtacc
      181 agccggaagt gacgcccggc accatccgtc acgccggtga tttcgccgct gaattcgccg
      241 ccagcctcac ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc
      301 cggtacggcg gcagcggcgc aggcagcacc tcatccgcct ccggcacccg ctcacaccag
      361 ccgagtaccc accacggccc ctgcggactg ttcgtcgcca gataacgatg cggactcagg
      421 cgaagctctt ccggcagcgc ctgccagagt gcggccagcc g
//
LOCUS       DAZGHF010000490          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZGHF010000490 DAZGHF010000000
VERSION     DAZGHF010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.494"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>459)
                     /locus_tag="ACHK3A_005404"
     CDS             complement(<1..>459)
                     /locus_tag="ACHK3A_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8743312.1"
                     /translation="DDIDAILAAADVGCFATYITRQGGVLIPRNTYLVRTAYETAEEA
                     NDYGEFPQRIYGVRAPSLGERYTICTHPDTWKLVRRKPENEDRTDEGFDVECGFSAPW
                     TRGNNCPPDQNLTINDPPERQPDGRREQRLTLPEGDLGEWLRSLTPTERK"
BASE COUNT           76 a          126 c          146 g          111 t
ORIGIN      
        1 gttttcgctc tgtgggggtc agtgacctta accattcacc caaatcacct tccggcaggg
       61 taagtcgctg ctccctgcgt ccgtccggct gcctttctgg cgggtcgttt atggtcagat
      121 tctggtcagg gggacagtta ttgccacgag tccaaggtgc ggaaaagccg cactcaacgt
      181 caaaaccctc gtccgtgcgg tcttcgtttt ccggcttacg tctgaccagt ttccaggtgt
      241 ccgggtgggt gcatatggtg tagcgctccc cgagtgacgg ggcgcggacg ccgtaaatgc
      301 gttgcgggaa ttcgccgtag tcgttggcct cttcggcagt ttcgtatgcg gtgcggacca
      361 ggtacgtgtt gcggggaatg agcacaccac cctggcgggt gatataggtg gcaaaacatc
      421 ccacgtcggc ggcggccagt atggcgtcaa tgtcgtcat
//
LOCUS       DAZGHF010000491          446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZGHF010000491 DAZGHF010000000
VERSION     DAZGHF010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.495"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(140..259)
                     /locus_tag="ACHK3A_005405"
     CDS             complement(140..259)
                     /locus_tag="ACHK3A_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO8743313.1"
                     /translation="MPLSYAGYHYFIMMSLVSGEHNTLALPIQEQSPGLVPAG"
     gene            complement(220..405)
                     /locus_tag="ACHK3A_005406"
     CDS             complement(220..405)
                     /locus_tag="ACHK3A_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743314.1"
                     /translation="MSRKYSSAMITLVILFCRRPHCRKISVQQYYQKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
BASE COUNT          139 a           97 c           85 g          125 t
ORIGIN      
        1 catgccgttc cccagacgca tgttgtaact cagcgttgca ccaagttcac gatatatact
       61 gcgggtatcg actgatttcg accccatgcc attggataaa tgatagtagc gtctaagtga
      121 ccttagctct gctgcaacat catcccgcag gcaccagacc aggggattgc tcctgaatag
      181 gcagcgcaag tgtattgtgt tcaccgctca ccaaagacat catgatgaaa taatgatatc
      241 ccgcataaga aagaggcatt ttttaaacgc agtgcgctga agtgtagttg gataaaaaat
      301 cactcttttc tgataatact gttgtacgct tatttttcga caatgaggcc gtcgacaaaa
      361 taaaatgact aatgtaatca ttgctgatga atattttctg ctcacataaa aatcacacaa
      421 taatattcat aaatcagatt atctta
//
LOCUS       DAZGHF010000492          442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZGHF010000492 DAZGHF010000000
VERSION     DAZGHF010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.496"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..314)
                     /locus_tag="ACHK3A_005407"
     CDS             complement(<1..314)
                     /locus_tag="ACHK3A_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743315.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARG"
BASE COUNT          101 a          146 c           85 g          110 t
ORIGIN      
        1 cctcgcgctt tctccgcggt cagcaggtcc gtgattttct ccccgtcaaa accccagcgc
       61 cccgagatgt cagctttgcg gttttcttcc gtatccgcca cctccaccac actctggccc
      121 actggccaca gaactgatgc caccctgctg atgccggtaa tgaccccggc actgttataa
      181 accaccttca gcgtctctgc tgaaaataac gactgacact cataccagtc ctgaccatcc
      241 tctgatttca ggtacatcgc acccggcaca tccggatttt ccggcgtgta ttgtctgaaa
      301 ttctttatgt gcatctgtaa gtcctcccgg ttacatgtct gaaagatgat gcgctttata
      361 atggaaaata ctcaaacccc gaagctcctg acgacacata ctcacccacg ctgactgtac
      421 gccactgaga accatcaaat gt
//
LOCUS       DAZGHF010000493          441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZGHF010000493 DAZGHF010000000
VERSION     DAZGHF010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.497"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>441
                     /locus_tag="ACHK3A_005408"
     CDS             <1..>441
                     /locus_tag="ACHK3A_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8743316.1"
                     /translation="WPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYS
                     HRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKG
                     YWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLV"
BASE COUNT          122 a           89 c          140 g           90 t
ORIGIN      
        1 gtggccggga ctgggaaatg aagtgacagc ccgcctgctg accgtgacgc gcaaaggaaa
       61 agtctgccat ctgctgacgt cgatgacgga cgccatgcgc ttccccggag gagaaatggc
      121 ggatctgtac agtcatcgct gggaaatcga actgggatac agggagataa aacagacgat
      181 gcaactgagc aggctgacgc tgagaagtaa aaagccggag cttgtggagc aagagctgtg
      241 gggtgtctta ctggcttata atctggtgag atatcagatg attaaaatgg cggaacatct
      301 gaaaggttac tggccgaatc aactgagttt ctcagaatca tgcggaatgg tgatgagaat
      361 gctgatgaca ttgcagggcg cttcaccggg acgtataccg gagctgatgc gcgatcttgc
      421 aagtatggga caacttgtga a
//
LOCUS       DAZGHF010000494          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZGHF010000494 DAZGHF010000000
VERSION     DAZGHF010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.498"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>430)
                     /locus_tag="ACHK3A_005409"
     CDS             complement(<1..>430)
                     /locus_tag="ACHK3A_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HFO8743317.1"
                     /translation="SQEERLSLISRANEALSLWDNWLHPFTSGSETGDDPAYDDNFQL
                     MREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLTRDGERGLSEGLLLLV
                     AMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGV"
BASE COUNT          101 a          128 c          109 g           92 t
ORIGIN      
        1 tccaccccgg gccataacag cagtgcatca gtcacttttg cactgttcag ccactccagc
       61 gcagctttcc gggcggcagg acgccgggga tgacatgcct gtccgtagcg ggtcagcatc
      121 gcaacaagaa gtaataagcc ttcggacaac cctctttcac catcccgggt gagacgggcc
      181 agcacgtacc aggtcacgac acgaatatct ctggcgcatt cgcaaaggca cttctgagcc
      241 agttcgcaca acagcgctga atcagtacct gaaatcttat tgatctcttc acgcatcagc
      301 tgaaaattat catcgtatgc aggatcgtcc ccggtttcag atccggaggt aaatggatgc
      361 agccagttat cccagagcga cagggcctca tttgcgcggg aaatgaggct cagcctttct
      421 tcctggctgg
//
LOCUS       DAZGHF010000495          426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZGHF010000495 DAZGHF010000000
VERSION     DAZGHF010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.499"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..225)
                     /locus_tag="ACHK3A_005410"
     CDS             complement(<1..225)
                     /locus_tag="ACHK3A_005410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743318.1"
                     /translation="MSPSVKCDRYTFSGLVGAGTGKIFIQPDGTGDALFVVRLSGEVV
                     TPQCQILLKGIAADAFDPRGQFFNSISSFGE"
     gene            224..>426
                     /locus_tag="ACHK3A_005411"
     CDS             224..>426
                     /locus_tag="ACHK3A_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8743319.1"
                     /translation="MASQLDFIINLTDQLTRPLRQVQESVTGFAERSQGAFARIGVGV
                     AGLWGVAEGIRAAMDPAWEMQKAL"
BASE COUNT           90 a          122 c          136 g           78 t
ORIGIN      
        1 ctccccgaaa ctggagattg agttaaaaaa ctgaccgcgc gggtcgaaag catccgccgc
       61 aatccccttg agcagtatct ggcattgcgg cgtcactacc tccccggaga gccggacgac
      121 gaagagagca tcgcccgtgc catctggctg gatgaatatt ttgcccgtac ccgcgccaac
      181 aagaccgctg aaggtatagc gatcgcattt aacggaaggt gacatggcca gccagctgga
      241 tttcattatc aatctgacag accagcttac ccgcccgctg cggcaggttc aggaatccgt
      301 gacgggattc gccgaacgct cgcagggcgc gttcgcgcgt atcggtgtgg gtgtggccgg
      361 actgtggggc gtggccgaag gtatccgcgc cgcgatggat ccggcgtggg agatgcagaa
      421 ggcgct
//
LOCUS       DAZGHF010000496          426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZGHF010000496 DAZGHF010000000
VERSION     DAZGHF010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.500"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..408
                     /locus_tag="ACHK3A_005412"
     CDS             <1..408
                     /locus_tag="ACHK3A_005412"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFO8743320.1"
                     /translation="ATQIISLDRLLDNIPESTINVLKTNEFVISSPDSFDIREENRTS
                     ILYEHNGELEIQLSIEVTRALNTDAEKALKLISEAANAASSSLMWDKGDFLIFNNRKT
                     LHGRREIKGKRWLQRCYGSKNIPLYKTINLEGL"
BASE COUNT          161 a           49 c           85 g          131 t
ORIGIN      
        1 gcgacgcaaa ttatttcatt ggatcgattg ctggataata ttccagaatc aacaataaat
       61 gttttgaaaa ctaatgagtt tgtgattagt tctcctgatt catttgatat aagagaagaa
      121 aatagaacat ctatattata cgaacataac ggagaattgg agattcaatt atcaatagag
      181 gttactagag cgttgaatac tgatgcagag aaggctttga aattaatttc tgaagcggca
      241 aatgcagcat catctagttt gatgtgggat aagggcgatt ttttaatttt caataacaga
      301 aaaactttac atggacggag agaaattaaa gggaaacgat ggctgcaacg ttgttatggt
      361 tcaaaaaata taccgctata taaaacaata aatttagaag gattataatt atttatggat
      421 gaatta
//
LOCUS       DAZGHF010000497          426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZGHF010000497 DAZGHF010000000
VERSION     DAZGHF010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.501"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>426
                     /locus_tag="ACHK3A_005413"
     CDS             <1..>426
                     /locus_tag="ACHK3A_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8743321.1"
                     /translation="KRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSG
                     DITDITTPDGRETKFYYNDGNQLTAVVYPDGLESSRAYDELDRLVSETSRSGETVRYR
                     YDDVHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGY"
BASE COUNT          101 a          111 c          156 g           58 t
ORIGIN      
        1 tgaagcgggt ggtgaaaaaa gaactggcgg acggcagcgt cacgcacagc gggtatgacg
       61 cggcaggaag gctcacggca cagacggacg cggcgggacg gcggacagag tacggcctga
      121 atgtggtgtc cggcgatatc acggatatca ccacgccgga cgggcgggag acgaaatttt
      181 actataacga cgggaaccag ttaacggcgg tggtgtaccc ggacgggctg gagagcagcc
      241 gggcatatga tgagctggac agactggtat cggagacatc gcgcagcggg gagacagtac
      301 gctaccgcta cgatgacgtg cacagtgagt taccggcgac gacaacggat gcgacgggca
      361 gcacccggca gatgacctgg agccgctacg ggcagttgct ggccttcacc gactgctcgg
      421 gctacc
//
LOCUS       DAZGHF010000498          416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZGHF010000498 DAZGHF010000000
VERSION     DAZGHF010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.502"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>416
                     /locus_tag="ACHK3A_005414"
     CDS             <1..>416
                     /locus_tag="ACHK3A_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
                     /protein_id="HFO8743322.1"
                     /translation="NQWKNLGTTPFPGTAGSSVIFAEQQIYILGGERKPGLRSVRSWT
                     GELSHDRIKWSELPPVASPEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHK
                     GLDKAYSKEIYQLIKNDWKKVGSLPEGLAYGVSLPW"
BASE COUNT          116 a           79 c          114 g          107 t
ORIGIN      
        1 aaccagtgga agaacctggg aacgactcca tttccgggaa ctgctggttc atcggtgatt
       61 tttgcagaac agcaaattta tatactggga ggggagagaa aaccgggcct tcgttctgtt
      121 agatcctgga cgggggagct ttcgcatgat cgaattaaat ggtctgaact tccaccagta
      181 gcttcacccg agggggtgtc aggagcatat gcctctgtta ttgatggaaa tatctttctg
      241 gccggtggtg catatttccc aggggcggca gaaaagtata gcaatggtga atactggtct
      301 cataaaggac tggataaagc ctattcaaaa gagatatatc agctaataaa aaatgactgg
      361 aaaaaagttg gttctttgcc ggaaggactt gcttatggtg taagcctgcc ctggca
//
LOCUS       DAZGHF010000499          415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZGHF010000499 DAZGHF010000000
VERSION     DAZGHF010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.503"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>415)
                     /locus_tag="ACHK3A_005415"
     CDS             complement(<1..>415)
                     /locus_tag="ACHK3A_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8743323.1"
                     /translation="MFPDAVLSLLAPFASTVQGGQRTRRLILLIWLCVLTALGISALN
                     NRDLIRQVSTDLQRWNAVPKGHYRPEAESLAALKQDALLLEQWQRQGVPLRYSLGYYP
                     GQRLWLALQQAIDTRVPPPSPEPEPVPEIVRLDSLA"
BASE COUNT           96 a          126 c          118 g           75 t
ORIGIN      
        1 gccaggctat cgagtcgtac aatttccggc accggttctg gttcagggga tggtggtgga
       61 acccgggtat cgatagcctg ctgcagggcc agccacagac gttgtcccgg gtaatagccc
      121 aggctgtaac gtaaaggcac tccctgacgc tgccactgct caagcagaag cgcatcctgt
      181 ttcagtgcgg ccagcgattc cgcctcagga cggtagtgcc ccttcgggac agcattccag
      241 cgctgtaaat cggtgcttac ctggcggatc agatcacgat tgttaagcgc agaaatcccc
      301 agcgcagtca ggacacacag ccatatcagc aaaatcagac gacgggtacg ctggcctccc
      361 tgtaccgtcg acgcaaaagg tgccagcagc gacagaaccg catccgggaa catcc
//
LOCUS       DAZGHF010000500          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZGHF010000500 DAZGHF010000000
VERSION     DAZGHF010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.504"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>414
                     /locus_tag="ACHK3A_005416"
     CDS             <1..>414
                     /locus_tag="ACHK3A_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO8743324.1"
                     /translation="CQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADL
                     WHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQIC
                     WHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT          106 a          104 c          118 g           86 t
ORIGIN      
        1 ctgccagacc tgggacgacg aaaacgtcca taaattgatg gatttgtcga tcaataaaaa
       61 ctggatcgac aaagaagaat atccgcaatc tgcagccatc gacctgcgtt gcgtaaacat
      121 ggttgccgat ctgtggcatg cgcctgcgcc gaaaaatggt caggccgttg gcaccaacac
      181 cattggttct tccgaggcct gtatgctcgg cgggatggcg atgaaatggc gttggcgcaa
      241 gcgtatggaa gctgcaggca aaccaacgga taaacccaac ctggtgtgcg gtccggtgca
      301 aatctgctgg cataaattcg cccgctactg ggatgtggag ctgcgtgaga tccctatgcg
      361 ccccggtcag ttgtttatgg acccgaaacg catgattgaa gcctgcgacg aaaa
//
LOCUS       DAZGHF010000501          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZGHF010000501 DAZGHF010000000
VERSION     DAZGHF010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.505"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..200)
                     /locus_tag="ACHK3A_005417"
     CDS             complement(<1..200)
                     /locus_tag="ACHK3A_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO8743325.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     GVERLFEGFAFLMGRLREKLDD"
     gene            complement(234..>413)
                     /locus_tag="ACHK3A_005418"
     CDS             complement(234..>413)
                     /locus_tag="ACHK3A_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO8743326.1"
                     /translation="TLEDWEPALLFEVRARRLKLLRMKAGRSESDKARLMPEMDTLLA
                     GLIAIDPARAMVLCG"
BASE COUNT          101 a          130 c          101 g           81 t
ORIGIN      
        1 tcatcatcca gcttttcacg caaacgtccc atcaggaagg caaagccttc aaacagacgt
       61 tccacaccgg gatcgcaggc gccaggctta tccagattca gcattgctgc ccgatccgga
      121 tgagcacggg caaactcttt accggcttcc cgcagatagc gcatctctgc ttcgtaatag
      181 cgcagggtta aatcatccat gcacggcatt cctgaaaatg attaaagaga acatcagcca
      241 cagagcacca tggcacgggc cgggtcaatg gcgatcagcc ccgccagcag cgtatccatc
      301 tcaggcatca gacgggcttt atcagattcg ctgcgaccgg ctttcatccg tagcagcttc
      361 aggcgtcggg cccggacttc aaacagcaaa gcgggctccc agtcttccag cgt
//
LOCUS       DAZGHF010000502          407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZGHF010000502 DAZGHF010000000
VERSION     DAZGHF010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.506"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..192)
                     /locus_tag="ACHK3A_005419"
     CDS             complement(<1..192)
                     /locus_tag="ACHK3A_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO8743327.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVDFLTNTDEG"
     gene            complement(189..>407)
                     /locus_tag="ACHK3A_005420"
     CDS             complement(189..>407)
                     /locus_tag="ACHK3A_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743328.1"
                     /translation="DGDGFGLFHINGTDSTAISWSSSEMALVLEMTTHSVAVKGIYTA
                     EDFIGEYQRLLVLYRAAFQAATAGECDA"
BASE COUNT           75 a          114 c          116 g          102 t
ORIGIN      
        1 gccttcgtct gtgtttgtca ggaagtcgac ggtctcttca tccagtcttg catcaatgcg
       61 aatgtcttcg ttgatgtagc tctgaatggc ctggcgtaat gcggttggtt gcgctctgcc
      121 gaacgtttca ggatcgataa ccggtagcgg ggcgttccac gggtacgcca gctgtgcatt
      181 gtggtcactc atgcgtcgca ctccccggca gtcgccgcct gaaacgccgc gcggtacagc
      241 acgagaaggc gctggtattc accgataaag tcttctgcgg tgtatatgcc tttcacggcc
      301 acgctgtgcg ttgtcatttc cagcacaagc gccatctctg acgacgacca gctgatggcg
      361 gtgctgtcgg tgccgttgat gtgaaataaa ccaaagccat cgccatc
//
LOCUS       DAZGHF010000503          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZGHF010000503 DAZGHF010000000
VERSION     DAZGHF010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.507"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>403
                     /locus_tag="ACHK3A_005421"
     CDS             <1..>403
                     /locus_tag="ACHK3A_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO8743329.1"
                     /translation="IVRVTIGEYGATGFINGTVCTCTVYGDTQYFHWRVDYSTKNKPD
                     TVSQRDASTTQKGVVQLSSDTGSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGV
                     PGPKGEKGDPGAQGPKGETGATGPAGPRGAQG"
BASE COUNT          116 a           92 c          138 g           57 t
ORIGIN      
        1 catagtccgg gtcaccattg gcgaatacgg tgcaacgggg tttattaatg gcacggtctg
       61 tacctgtacg gtttacggcg acacacagta tttccactgg cgggtggatt acagcacaaa
      121 aaacaaaccg gatacggtca gccagcggga tgccagcacg acgcagaaag gtgtggtgca
      181 gttaagcagt gatactggca gtaatgacga aacaaaggca gccacgccga aggccgtgaa
      241 ggcggcaatg gatgtggcaa atgaagcgaa aacaaaggca gaagaggctg cagcaggagg
      301 tggtgttccc ggtccgaaag gagagaaagg cgaccccgga gcacagggtc cgaaaggtga
      361 aacgggagca acaggccccg ctggtccgcg gggagcgcag gga
//
LOCUS       DAZGHF010000504          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZGHF010000504 DAZGHF010000000
VERSION     DAZGHF010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.508"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>397
                     /locus_tag="ACHK3A_005422"
     CDS             <1..>397
                     /locus_tag="ACHK3A_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8743330.1"
                     /translation="LMKDEQTELLHLEKDIGRRVVGQDVALESIAQRLRAAKTGLTSG
                     NGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPG
                     YVGYGQGGILTEAVRKRPYSVVLLDEVEKA"
BASE COUNT           92 a           97 c          135 g           73 t
ORIGIN      
        1 ccctgatgaa ggacgaacag acggaactgc tgcatctgga aaaggatatc ggcagacggg
       61 tggtcggaca ggacgtggca ctggaatcca ttgcgcagcg tctgcgtgcg gcgaaaacgg
      121 gtctgacatc cggtaacggc ccccaggggg tgttcctgct ggttgggccc agtggtgtgg
      181 gaaaaaccga aacggcactg gcgctggcag acgtgatgta cggcggcgaa aaatcactga
      241 ttaccatcaa tctgtcggaa taccaggagc cccatacggt ttcccagctt aagggttcac
      301 cgcccgggta cgtgggatac ggtcagggcg gtattctgac ggaagctgtg cgtaaacgcc
      361 cttacagcgt ggtgctgctg gatgaagtgg aaaaggc
//
LOCUS       DAZGHF010000505          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZGHF010000505 DAZGHF010000000
VERSION     DAZGHF010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.509"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(200..>397)
                     /locus_tag="ACHK3A_005423"
     CDS             complement(200..>397)
                     /locus_tag="ACHK3A_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743331.1"
                     /translation="ESSNREFIRYRFAQCSFALTWGARVGRLAFSRLYPCYTELNKIR
                     AMQLNNAYISDFQNPDEISSK"
BASE COUNT          124 a           61 c           80 g          132 t
ORIGIN      
        1 gtcattgagt cagtttcgat atcgaatgct aaatctacaa agcaataatc tacgtagaac
       61 ataaatctac gtatatcttc tggtatggct gtggaaaaat aacggtattg cgtgtaagtg
      121 gtttgatcat cgaattcgtc tgattgtact gaatatgtaa ttgtggccag tataaatagt
      181 aatgttaatt ttataataat catttgctgg atatctcatc tggattttga aaatctgaaa
      241 tgtaagcatt gtttagttgc atggctctta ttttgtttaa ctcagtatag catggatata
      301 gtcgagagaa tgccagacgt cctactctgg ccccccaggt tagagcaaaa gagcattgag
      361 caaatctgta acgtataaat tcccgattag aagattc
//
LOCUS       DAZGHF010000506          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZGHF010000506 DAZGHF010000000
VERSION     DAZGHF010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.510"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>396)
                     /locus_tag="ACHK3A_005424"
     CDS             complement(<1..>396)
                     /locus_tag="ACHK3A_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743332.1"
                     /translation="LPLHLAALEGDENAFFSAEVNMVRRELQSVPGNSVNCMCWASLA
                     ALAFDAEERVERQKGNNENIATYDESIATYAFRAACTVSRCLSRNASGGIPRSYPEWK
                     AILFPRDGADIFPDGWLENMTRRAERVFTS"
BASE COUNT           98 a          120 c           93 g           85 t
ORIGIN      
        1 actcgtaaaa acacgttcag cccgacgggt catattttcc agccacccgt cggggaaaat
       61 gtcagcacca tcacggggaa acaaaatcgc tttccattca gggtatgagc gcggaatacc
      121 tccggaagca ttccggctca gacagcgact cacagtgcag gcagcacgga atgcatatgt
      181 tgcgatgctc tcatcatatg ttgcgatgtt ctcattattg cctttctgcc gttccacccg
      241 ctcttcagca tcaaatgcca gtgcagcaag gcttgcccag cacatgcagt tcactgaatt
      301 ccccggaacg ctctgcagct cccggcgcac catgttgacc tcagcactga aaaaagcgtt
      361 ctcatcgcct tcaagggcag ccagatgcag cggaag
//
LOCUS       DAZGHF010000507          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZGHF010000507 DAZGHF010000000
VERSION     DAZGHF010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.511"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>395
                     /locus_tag="ACHK3A_005425"
     CDS             <1..>395
                     /locus_tag="ACHK3A_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8743333.1"
                     /translation="IFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVGTTDTLR
                     EMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTPWLNTAP
                     GLFIDGYTSAAASTVPSLSRTLIYDYEQNP"
BASE COUNT           93 a           97 c          108 g           97 t
ORIGIN      
        1 attttctggc ggacacacca ccgcggtcac cgtaactggc tggcactgct gctgttcgta
       61 ttatgctctg taaacagctg gccgttgcgg atggttaaag gaaccgttgt ggggacaact
      121 gacacattgc gtgaaatgca gcgttatgaa caactgagtc agcacagggc tgacagctgg
      181 aaaatcctgc cgggagcacc gttgtatgac acgattgtga ttgtcactgg tgagagtgtg
      241 cgcagggatt atatgtcggt atatggttat cccgtaccaa ccacaccgtg gctgaatacg
      301 gctcccggtt tatttattga cggctataca tcggcagcag ccagtaccgt accttccctg
      361 agccggacac tgatttatga ctatgagcag aaccc
//
LOCUS       DAZGHF010000508          392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZGHF010000508 DAZGHF010000000
VERSION     DAZGHF010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.512"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>392)
                     /locus_tag="ACHK3A_005426"
     CDS             complement(<1..>392)
                     /locus_tag="ACHK3A_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743334.1"
                     /translation="SQTALLALDGEGIFMQNMVISPSMQFQEKDQSGQTSSTANAEQG
                     IKAKELRVTGMITFDNERALQRIFQLASATTGDGALKVYRIANATAAAINFREGTFSG
                     QIDAQQQTDRLAWQVSFTLRERRSVPEK"
BASE COUNT           69 a          107 c          120 g           96 t
ORIGIN      
        1 gtttttccgg tacgcttcgt ctttcgcgta atgtgaagct gacctgccag gcgaggcggt
       61 cggtctgctg ctgtgcgtct atctgcccgg aaaatgtccc ttcacgaaag ttaatggcgg
      121 cagcggtggc gtttgcgata cggtaaactt tcagggcacc gtccccggtg gtggcagagg
      181 ccagctggaa aatgcgctga agggcccgct cattgtcaaa ggtgatcatc ccggttacgc
      241 gaagctcttt ggccttgatg ccctgctcgg cgttcgcggt gctcgatgtc tggccggact
      301 ggtccttttc ctggaactgc atcgagggcg aaatcaccat gttctgcatg aaaataccct
      361 caccatcaag ggcgagcagt gcggtctggc tc
//
LOCUS       DAZGHF010000509          388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZGHF010000509 DAZGHF010000000
VERSION     DAZGHF010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.514"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>388)
                     /locus_tag="ACHK3A_005427"
     CDS             complement(<1..>388)
                     /locus_tag="ACHK3A_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFO8743335.1"
                     /translation="ANVGIPADKVTQDGEYTVKAEITDPAGNTSGQGKATQFGVDTVA
                     PSEPALKAENDGSVSATLPDGANKGDKVEVTFTDEEGNEQKVTLEKGDGNWSSDKPEL
                     IPDSTDNKVTVPADKVKNNTEVTATAK"
BASE COUNT           64 a          121 c           80 g          123 t
ORIGIN      
        1 ctttcgctgt tgccgtcacc tctgtgttgt tcttcacttt gtctgccgga actgtcactt
       61 tattatcggt gctgtccggg atcagttcag gcttgtcgga actccagttg ccatcccctt
      121 tctccagcgt gactttctgc tcattaccct cttcatccgt aaaggtcact tccaccttgt
      181 cacctttgtt cgcaccatct ggcagagtcg ctgatacaga accatcattc tcagccttca
      241 gtgctggttc tgacggagct acagtatcca cgccaaactg ggtagcttta ccctgaccac
      301 tggtgttgcc tgccgggtct gtgatttcag ccttaactgt gtattcccca tcctgcgtca
      361 ctttatccgc aggaatgccc acattcgc
//
LOCUS       DAZGHF010000510          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZGHF010000510 DAZGHF010000000
VERSION     DAZGHF010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.515"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..271
                     /locus_tag="ACHK3A_005428"
     CDS             <1..271
                     /locus_tag="ACHK3A_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO8743336.1"
                     /translation="VEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDDLEEHLTR
                     ITQHPLMSDLIYYPAKKGDDKPENILKIVKEWRRSQGLSLFKDSE"
BASE COUNT          136 a           64 c           89 g           96 t
ORIGIN      
        1 agtagaagat tacacagagc gggaattcat tgagttgctt ggcgagttta caaatccaac
       61 cggggataac gctcaattaa aaggggaaga gcttgacaaa tattgggatg atctagaaga
      121 acatcttacc agaattacac aacatccgtt gatgagcgat cttatctact atcctgcaaa
      181 gaagggagat gataagcctg aaaacatcct gaaaatcgtc aaagaatggc ggcgttcgca
      241 aggattgtct ttgttcaaag attcagaatg aattctaaac agaatctgtc acctgaaaat
      301 catcacgttc tggttaagtg atgattttcg ctacgagaag gttacggtgg cgattgcagt
      361 gataataagc aatgtaaaaa gaaaa
//
LOCUS       DAZGHF010000511          376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZGHF010000511 DAZGHF010000000
VERSION     DAZGHF010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.516"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>376
                     /locus_tag="ACHK3A_005429"
     CDS             <1..>376
                     /locus_tag="ACHK3A_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8743337.1"
                     /translation="GWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIR
                     YRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEGGLKRVVKKEHA
                     DGSVTQSQFDAVGRLKAQTDAAG"
BASE COUNT           93 a          103 c          129 g           51 t
ORIGIN      
        1 ggctggacgc cgcgcggcga actggcggcg gtgtatgacc gcagcaatac acaggtgcgc
       61 agctttactt acgatgataa ataccggggc cggatggtgg cgcaccgtca cacgggccgg
      121 ccggaaatcc gttaccgtta cgacagtgac gggcgggtga cagaacagct aaacccggca
      181 ggcttaagct acacgtatca gtatgagaaa gaccacatca ccatcaccga cagcctggac
      241 cgccgtgaag tgctgcacac gcagggcgaa ggcgggctga agcgggtggt gaaaaaggaa
      301 cacgcggacg gcagcgtcac gcagagtcag tttgacgcgg tgggcaggct taaggcacag
      361 acggatgccg caggcc
//
LOCUS       DAZGHF010000512          376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZGHF010000512 DAZGHF010000000
VERSION     DAZGHF010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.517"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT          111 a           64 c           63 g          138 t
ORIGIN      
        1 tgtgtcagag ggttgccgaa catttttatt cttagacaaa agaaccttat taattgcctt
       61 tatcctttcc cggtcaagtt caaaaccatg ctcctcaatt ataattgaat tatatgtgtt
      121 atttattttt aaatttgtgt tattaggaat aggatcgtat ttttcctcca tatagtattc
      181 attgccagta aaaaaatgtt ctagtaccag ggggattgtc ggatgtgttt tcagaagttc
      241 taatagggtc ctgttatata tctgggcacc agaaaaaata cacgcataag tatgatttct
      301 tattttacta agtttttctt tccaaaactc aagccaggac catgctgccg caaagtttaa
      361 taacaatgtc ctttga
//
LOCUS       DAZGHF010000513          375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZGHF010000513 DAZGHF010000000
VERSION     DAZGHF010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.518"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>375)
                     /locus_tag="ACHK3A_005430"
     CDS             complement(<1..>375)
                     /locus_tag="ACHK3A_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743338.1"
                     /translation="LLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRH
                     TPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVILPETGTSTVNLINGSGKPV
                     RVDITAHPAPDRIQVSVLPDGVE"
BASE COUNT           61 a          124 c          112 g           78 t
ORIGIN      
        1 ctccacgcca tccggcagga cgctgacctg tatccggtcc ggggcggggt gtgcggtgat
       61 gtccacgcgc accggcttac cgctgccgtt aatcaggttc accgtcgatg tccctgtctc
      121 cggcagtatc acctcacggt ccagcgtcag ggtacggctg gcggcatcga tggacaggat
      181 gcgtccgccg gtcagggtcc cggcgtagtc gttatcacag atttcaatga tgtcaccggg
      241 cgtgtgccgc agcccctgtg acccgagcgt gaaatccacc gtctgcgttt ccagcagttc
      301 tgtctttatc acccacagtc cggcacggtg ggcctgaccg cggctggtac agccaaacgc
      361 atccatcttc agcag
//
LOCUS       DAZGHF010000514          375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZGHF010000514 DAZGHF010000000
VERSION     DAZGHF010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.519"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>375
                     /locus_tag="ACHK3A_005431"
     CDS             <1..>375
                     /locus_tag="ACHK3A_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8743339.1"
                     /translation="LLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRH
                     TPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVTLPETGTSTVNLINGSGKPV
                     RVDITAHPAPDRIQVSALPDGVE"
BASE COUNT           80 a          113 c          122 g           60 t
ORIGIN      
        1 ctgctgaaga tggacgcgtt cggctgtacc agccgcggtc aggcccaccg tgccggactg
       61 tgggtaataa agacagaact gctggaaacg cagacggtgg atttcacgct cgggtcacag
      121 gggctgcggc acacgcccgg tgacatcatt gaaatctgtg ataacgacta tgccgggacc
      181 ctgaccggcg gacgtatcct gtccatcgat gccgccagcc gcacactgac gctggaccgt
      241 gaggtgacac tgccggagac aggtacatcg acggtgaacc tgattaacgg cagcggtaag
      301 ccggtgcgcg tggacatcac tgcacacccc gccccggacc ggatacaggt cagcgccctg
      361 ccggatggcg tggag
//
LOCUS       DAZGHF010000515          374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZGHF010000515 DAZGHF010000000
VERSION     DAZGHF010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.520"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            24..100
                     /locus_tag="ACHK3A_005432"
     tRNA            24..100
                     /locus_tag="ACHK3A_005432"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHK3A_005433"
     tRNA            143..218
                     /locus_tag="ACHK3A_005433"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT          108 a           79 c           90 g           97 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
      361 caacacgatg atga
//
LOCUS       DAZGHF010000516          370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZGHF010000516 DAZGHF010000000
VERSION     DAZGHF010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.521"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            11..>370
                     /locus_tag="ACHK3A_005434"
     CDS             11..>370
                     /locus_tag="ACHK3A_005434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFO8743340.1"
                     /translation="MTSKTDGVADAPALTIPEVADSVANAAELKDGLQAEVKLPAGTV
                     EGAVITLTVTHPDKTTRTETHTVSKDEAADGTVSMVVPKGSVVDGQNSVSVSLTQGSN
                     PAKAGNKVEFVVDGQVPG"
BASE COUNT           95 a           75 c          120 g           80 t
ORIGIN      
        1 tccggtcact gtgacgtcaa aaactgatgg tgtggcagat gctccggccc tgaccattcc
       61 agaagtggct gatagtgtgg ccaatgcggc tgagctgaaa gatggtttac aggcagaagt
      121 caagttgccg gcaggaacag tggaaggtgc tgtcattaca ctgacagtga ctcatccgga
      181 taaaaccacc agaaccgaga cgcatactgt tagcaaagat gaggctgcag acggtactgt
      241 gtcaatggtt gttccgaagg gaagtgttgt tgacggtcag aacagtgtga gtgtcagcct
      301 gactcagggc agtaacccgg ctaaagcggg gaataaagtg gagtttgtgg ttgacgggca
      361 ggttccgggt
//
LOCUS       DAZGHF010000517          367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZGHF010000517 DAZGHF010000000
VERSION     DAZGHF010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.522"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>367)
                     /locus_tag="ACHK3A_005435"
     CDS             complement(<1..>367)
                     /locus_tag="ACHK3A_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO8743341.1"
                     /translation="IRQNHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASN
                     VRSLILWTERGAANHAKLLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVI
                     EAEERAEAMALENKTLSATV"
BASE COUNT           82 a           90 c           89 g          106 t
ORIGIN      
        1 ctacggttgc agagagcgtt ttattttcca gggccatagc ttcagcccgt tcttcggcct
       61 caataaccat caacgcgagt tccttgcgag tgagtacgcg ctgaacatcg atgttttccc
      121 gctgagtgaa gtagaactcc acaaggtctt cgtgatagtt ccacgcctga tccgtttcca
      181 gcaattttgc gtggttggct gcgccgcgtt cggtccagag gatgagggat cgaacattgc
      241 tggcaatttt cacagagtag ctttgagata ctctgtgctt aagttcacgt agttcatcgc
      301 cttccagcaa aaaataatgc tttccaacaa cgaatcgttc ttcgtttcgg gaatgattct
      361 ggcgaat
//
LOCUS       DAZGHF010000518          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZGHF010000518 DAZGHF010000000
VERSION     DAZGHF010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.523"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            228..>366
                     /locus_tag="ACHK3A_005436"
     CDS             228..>366
                     /locus_tag="ACHK3A_005436"
                     /inference="COORDINATES: protein motif:HMM:NF015490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743342.1"
                     /translation="MSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESG
                     LY"
BASE COUNT          115 a           72 c           97 g           82 t
ORIGIN      
        1 tctggagaca ttgataatat tggtgagtgt caaattttca tcaggtataa acacagctgg
       61 attgagaata aattcaaaga aaaaataatt gaataaccat gttatgtata gacattttag
      121 atggaacgaa ccagaggaag gcatgcctcc gacttgggtt atatgggcac ggagttttag
      181 tgtagtgaac tcaacgacgc aaaccgaata acctgccgct ggcgcttatc agcacggaag
      241 gggcaacaga atggtgcgca gaatacgatg aatggggcaa cctgctgaat gaagagaacc
      301 cgcaccagct gcagcagctc atccgcctgc cgggtcagca gtatgatgag gagtccggcc
      361 tgtatt
//
LOCUS       DAZGHF010000519          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZGHF010000519 DAZGHF010000000
VERSION     DAZGHF010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.524"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..256)
                     /locus_tag="ACHK3A_005437"
     CDS             complement(<1..256)
                     /locus_tag="ACHK3A_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743343.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIE"
BASE COUNT          104 a          103 c           67 g           85 t
ORIGIN      
        1 cctcaatacg caccaccaca atggcttcct gtgcattcag taaaagaagc acaggaaccg
       61 caagtgaagg aatatcttcc ggagcccgcc gggaaagatg attatcaaac ccttcactgc
      121 gaagaaactc gccaacagaa cgaaaatcaa tcccggagac atctgtccgg actgtaccgg
      181 cagccagcgt ctcagcagat acaggtgtac ccagtaatgc cgttacctgt gataatcctt
      241 caaaaatact cttcacttat ttatctgtcc ttctgccctg ttactttttc ttctgcacca
      301 gaaagattca ctccgctcca tttcaggagc attccctggg aatacagata atccagcat
//
LOCUS       DAZGHF010000520          357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZGHF010000520 DAZGHF010000000
VERSION     DAZGHF010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.525"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..131)
                     /locus_tag="ACHK3A_005438"
     CDS             complement(<1..131)
                     /locus_tag="ACHK3A_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO8743344.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVSFIMQSNALHRLN"
BASE COUNT           77 a           84 c          113 g           83 t
ORIGIN      
        1 tcgttaagtc gatgtaatgc gtttgactgc atgatgaagg agacagatgc agcccgatgg
       61 agtctgcggt tgatttcagt ccggatgtga agacgctcag cgcgggcgcg ctgttgcttg
      121 cggtttgcca tggtgtggcc tcttgtgtag taagttttga aaactcacca tccagaggtt
      181 gcaattctgg gtggtgagac gtacagggtt gcaaccaccg gctacacaag accccggccc
      241 gaccgaagtc ggccctgtac gccccaccat aatttcggtg cgaaaaagat gtgacgatgc
      301 ggtacgcaca aaaaaaccgc tggcgcggtt gtgcgcttgt gtagtcagcg ggggttg
//
LOCUS       DAZGHF010000521          351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZGHF010000521 DAZGHF010000000
VERSION     DAZGHF010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.526"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>351
                     /locus_tag="ACHK3A_005439"
     CDS             <1..>351
                     /locus_tag="ACHK3A_005439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743345.1"
                     /translation="AYDSYCDFLKLDDAVVLQVKYECFSGYDSVSTFDGVMDGEFSFS
                     RMEPRPVYKLPVLIETQLYDPGKIIVDVFVPDAAKKQLKLKDTLELKQDEFVCLLQSG
                     CCWVKRREAGDKKKP"
BASE COUNT           95 a           50 c           83 g          123 t
ORIGIN      
        1 gcgtatgatt cttattgcga ttttttaaag cttgatgatg ctgttgttct tcaggtgaaa
       61 tatgagtgct tttctggtta cgattctgta agtacttttg atggtgtgat ggatggtgag
      121 ttttcttttt ctcgtatgga acccagacct gtctataaat tacctgtgct gattgaaact
      181 caactgtatg accccggaaa gattattgtt gatgtttttg ttcctgatgc tgcgaaaaaa
      241 caattaaaac ttaaagacac cttagagctg aagcaggatg aatttgtctg tttacttcag
      301 tcagggtgtt gttgggttaa acgaagggaa gcaggagata agaaaaaacc t
//
LOCUS       DAZGHF010000522          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZGHF010000522 DAZGHF010000000
VERSION     DAZGHF010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.527"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            64..>347
                     /locus_tag="ACHK3A_005440"
     CDS             64..>347
                     /locus_tag="ACHK3A_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO8743346.1"
                     /translation="MLWTENDAANTSQWNGYPLQIGRFRKDKAMPALISGEKSAALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEES"
BASE COUNT          126 a           69 c           64 g           88 t
ORIGIN      
        1 atcaacattc atttgtccag cactccatac tttcagccgt tggattggta tataataaaa
       61 gccatgcttt ggactgaaaa cgatgcggca aatacttctc agtggaatgg ttatccatta
      121 caaattggtc gttttcggaa agataaagcg atgcctgctt taatctctgg agaaaaatcc
      181 gcagcccttg ttaccccacc acaatggaga aacaaagcat tcaatgggtt aaaagatcca
      241 gaaagaaatt actgggcaaa agaacaaata actggctctc cagaagagaa tatcaaagca
      301 gcaattactt acttaatgat gaaactttct aatacaaagg aagaatc
//
LOCUS       DAZGHF010000523          341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZGHF010000523 DAZGHF010000000
VERSION     DAZGHF010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.528"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..212
                     /locus_tag="ACHK3A_005441"
     CDS             <1..212
                     /locus_tag="ACHK3A_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO8743347.1"
                     /translation="TKNNPGKNLDKIHPGDILYYQKASMKVIITGWKPITIKNVAMNY
                     NGGGDPKYAIKLQFVYTLLTKNRVL"
     gene            215..>341
                     /locus_tag="ACHK3A_005442"
     CDS             215..>341
                     /locus_tag="ACHK3A_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743348.1"
                     /translation="MGKITIILLLTFSCTKCLAFDISMSKECSGVDELEFVDCVKN"
BASE COUNT          132 a           49 c           59 g          101 t
ORIGIN      
        1 taacgaaaaa caaccctgga aaaaatctcg ataaaataca cccgggagat attttatatt
       61 atcaaaaagc atccatgaaa gtaataatta ctggatggaa acctatcaca ataaaaaatg
      121 tagcgatgaa ttataatggt ggtggagatc ccaagtatgc aataaaactt cagttcgttt
      181 atacattgct cacaaaaaac agagtgttat gagaatggga aaaataacaa ttattctact
      241 gcttacattc tcatgtacaa aatgtctggc atttgacatt tccatgtcta aagaatgtag
      301 tggcgttgat gaacttgaat ttgttgattg tgttaaaaat t
//
LOCUS       DAZGHF010000524          337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZGHF010000524 DAZGHF010000000
VERSION     DAZGHF010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.529"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            97..253
                     /locus_tag="ACHK3A_005443"
                     /pseudo
     CDS             97..253
                     /locus_tag="ACHK3A_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
BASE COUNT           88 a           86 c           87 g           76 t
ORIGIN      
        1 gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtcacacaa ccgtaggtta
       61 gcctcttacg tgccggaagg caaggagaag cagtctatga agcagcaaaa ggcgatgtta
      121 attcgccctg atcgtcatct gtttaactgt catagtgacg gcactggtaa cgaggaaaga
      181 cctctgcgag gtacggatcc gaaccggcca gacggaggtc gctgtcttcg tagactacga
      241 atctgagaag taagagacca ggcgggggag taatctcccg ccacctctga tgtgtcaggc
      301 atcctcaacg cacccgtact ttatttgtca tatgctc
//
LOCUS       DAZGHF010000525          336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZGHF010000525 DAZGHF010000000
VERSION     DAZGHF010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.530"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>336
                     /locus_tag="ACHK3A_005444"
     CDS             <1..>336
                     /locus_tag="ACHK3A_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743349.1"
                     /translation="KQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGY
                     CSAQGEQWEYRYDASGRRTEKRCDRKKIRFTYLWDGDNIAEIREYRDDKLYSVRHLVF
                     NGFELISQQ"
BASE COUNT           88 a           89 c          109 g           50 t
ORIGIN      
        1 gaaacaggcc ggaaacacgc agtatgacta tgacgccgca ggccggatgg tcagccgcac
       61 cagacaccgt gacggctacc gcccggaaac agagcggttc cggtgggaca gccgtgacca
      121 gctgaccggg tattgcagcg cacagggtga gcagtgggaa taccgctacg acgccagcgg
      181 caggcggacg gaaaaacgct gcgaccggaa gaaaatccgt tttacgtacc tgtgggacgg
      241 cgacaatatt gcggaaatcc gggaataccg cgatgataaa ctgtacagcg tacggcacct
      301 ggtgtttaac ggctttgagc tgataagcca gcagtg
//
LOCUS       DAZGHF010000526          325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZGHF010000526 DAZGHF010000000
VERSION     DAZGHF010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.531"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>325
                     /locus_tag="ACHK3A_005445"
     CDS             <1..>325
                     /locus_tag="ACHK3A_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HFO8743350.1"
                     /translation="LNKDTKCFDIIFLGSDQIWNANYTNGVDPNYYGQGPYCKAHKLV
                     SYAASMGKLCLGKYEEQAFLSLINNIQQIGVRENYLKTYIEEKTDLKCDVNLDPTLLL
                     TKSDW"
BASE COUNT          116 a           42 c           65 g          102 t
ORIGIN      
        1 cattgaataa agatactaaa tgtttcgata ttatctttct tggtagtgat cagatatgga
       61 atgcaaatta tactaacgga gtagacccta actattatgg acaaggacct tattgtaaag
      121 cgcacaaatt agtttcttat gcggcaagta tgggtaagct gtgtttggga aaatatgagg
      181 aacaagcatt cttatctttg ataaataata tacagcagat tggtgttaga gaaaattatt
      241 taaagacgta catagaagaa aaaaccgatt taaaatgtga tgttaatctt gatccgactc
      301 ttctgcttac taaaagcgat tggga
//
LOCUS       DAZGHF010000527          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZGHF010000527 DAZGHF010000000
VERSION     DAZGHF010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.532"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            83..196
                     /locus_tag="ACHK3A_005446"
                     /pseudo
     CDS             83..196
                     /locus_tag="ACHK3A_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          119 a           56 c           51 g           97 t
ORIGIN      
        1 acaaaaatca ttgtaaacca tgccatcttt ttagagatta ccaacagaaa attccttgtt
       61 cgaaacttag caaatgcaag ctatggaaaa acttacaacg gaactacact ccttaagtaa
      121 gatgggccga agacatgtaa tctctatttt agccgaaata gctaatggct atgatgattt
      181 tagtgacatg ttgatttagt tagaatttca cccgaatcat aagattctga tatcttaaat
      241 aagaaaatcc cgttcgacgt tctgaaaaag aaatcacaaa atattagcag atacttattt
      301 cgtgactgta tattatccga tga
//
LOCUS       DAZGHF010000528          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZGHF010000528 DAZGHF010000000
VERSION     DAZGHF010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.533"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>323)
                     /locus_tag="ACHK3A_005447"
     CDS             complement(<1..>323)
                     /locus_tag="ACHK3A_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743351.1"
                     /translation="VRYAALPEYQTDLTKALKGAVNKLTTRVEGIVHPLPPALPCWLV
                     LDCDNDLYPLIEEQLKADLSLKTGRIFRLMTGKGLGAFDAWLDKRWDTPGILVVITLS
                     LPASP"
BASE COUNT           72 a           95 c           85 g           71 t
ORIGIN      
        1 gggagaggca ggtaacgaca gcgtgatgac aaccagaatg cccggggtgt cccatcgctt
       61 gtccagccag gcatcaaacg cccccagccc ttttccggtc atcaggcgaa aaatcctgcc
      121 cgtcttgagt gagaggtcgg ctttaagctg ctcttcaatc aggggataca gatcgttatc
      181 acaatccagc accagccagc atggtagcgc gggcggaagc ggatgaacta tcccctctac
      241 acgcgtggtc agtttattca ccgccccttt cagagccttc gttaaatctg tctggtattc
      301 tggtaatgcc gcgtacctga ccg
//
LOCUS       DAZGHF010000529          318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZGHF010000529 DAZGHF010000000
VERSION     DAZGHF010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.534"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            14..>318
                     /locus_tag="ACHK3A_005448"
     CDS             14..>318
                     /locus_tag="ACHK3A_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743352.1"
                     /translation="MHTQKNRLPAFAWSGYSFPAPHKTGAGITTPPTTRAHNRASGFF
                     VRTVSPRLFRVRIMAGRTGPTSVGPGSLVAGCGNPVRLATLRLPPRVGEFSELTTRG"
BASE COUNT           63 a           93 c           86 g           76 t
ORIGIN      
        1 ctgagtgatc atcatgcaca cacaaaaaaa ccgcttgcca gcgttcgcct ggtcgggtta
       61 cagttttccc gcacctcata aaacgggtgc cgggattacc accccgccga ctacaagagc
      121 gcacaaccgc gccagcggtt tttttgtgcg tactgtatcg ccacgtcttt ttcgcgtcag
      181 aattatggcg gggcgtacgg ggccgacttc ggtcgggccg ggatctcttg tagccggttg
      241 tggtaaccct gtacgtctcg ccaccctgag attaccgcct cgggttggtg agttttcaga
      301 acttactaca agaggcca
//
LOCUS       DAZGHF010000530          317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZGHF010000530 DAZGHF010000000
VERSION     DAZGHF010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.535"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>317
                     /locus_tag="ACHK3A_005449"
     CDS             <1..>317
                     /locus_tag="ACHK3A_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO8743353.1"
                     /translation="VRTRRDEIDWVLTDKTHQTGSDISPEALKSRLTERYFTDFGNAW
                     LNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSPLVALMNTLNYQGKTGQKGEALADSVV
                     DSA"
BASE COUNT           81 a           81 c           90 g           65 t
ORIGIN      
        1 gtcagaacac gccgcgatga aattgactgg gtactgacgg ataaaacaca tcagacaggg
       61 agcgatatct ctccggaggc cctgaagagc cgcctgactg aacgctattt taccgatttt
      121 ggtaatgcct ggctgaacat gctcaacagc atacagtggc atgaacccac gtcactttcc
      181 ggagccattg cccagttgag tctgctcggt gacgtccgcc agtctccgtt agttgcactg
      241 atgaacacgc tgaactacca ggggaaaacg gggcagaagg gggaggcact ggctgattca
      301 gtcgtggatt cagcgaa
//
LOCUS       DAZGHF010000531          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZGHF010000531 DAZGHF010000000
VERSION     DAZGHF010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.536"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>314
                     /locus_tag="ACHK3A_005450"
     CDS             <1..>314
                     /locus_tag="ACHK3A_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743354.1"
                     /translation="LWLVRGGKATQPDGHTLARLWASLPPDIRLSPHLYLATSSAQGP
                     WWILGWSERVPGAEDVLPAPLPPYRMLTGLADRFGQTLTYRREAAGDLAGEITGVTDG
                     AGR"
BASE COUNT           43 a           96 c          127 g           48 t
ORIGIN      
        1 ctgtggctgg tgcgtggcgg taaggcaacg cagccggacg gccacacgct ggcacggctg
       61 tgggcgtcgc tgccgccgga tatccggtta agcccgcatc tttacctggc gaccagcagc
      121 gcacaggggc cgtggtggat actggggtgg tctgagcggg tgccgggtgc tgaggacgta
      181 ctgccagcgc cgctgccgcc gtaccggatg cttaccgggc tggcggaccg cttcgggcag
      241 acgctgacgt accggcgtga ggccgccggt gacctggccg gggaaatcac cggcgtgacg
      301 gacggtgccg ggcg
//
LOCUS       DAZGHF010000532          310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZGHF010000532 DAZGHF010000000
VERSION     DAZGHF010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.537"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..264)
                     /locus_tag="ACHK3A_005451"
     CDS             complement(<1..264)
                     /locus_tag="ACHK3A_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO8743355.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPG"
BASE COUNT           89 a           75 c           85 g           61 t
ORIGIN      
        1 gcccggcagc atgatgtcca ggcgattcac aatctggtga agaggttctt tacgctcaag
       61 cgccatgcca acaatacacc agaccatcat ttcgagggga agacggcgct tgcgtagcgt
      121 tacagtacct gattcggcaa ggcaacgaga gatgagttcg gggtcgaggt aatcccccag
      181 agaagtcagt gggttacgca gagaatcgta acgggatacc agatcaagag cctgtccaat
      241 gtgcataaaa aaatccggaa acaagtgagc gtttccggat tcttacacag ccactggatc
      301 ggtcaactga
//
LOCUS       DAZGHF010000533          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZGHF010000533 DAZGHF010000000
VERSION     DAZGHF010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.538"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>308
                     /locus_tag="ACHK3A_005452"
     CDS             <1..>308
                     /locus_tag="ACHK3A_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFO8743356.1"
                     /translation="FKSHPWERQKNNVRSALTLDKINEHIKSLSLDKSSRVLLTEHFN
                     LEGLIKQSDYIVTLCSQSAIEAAFWGMKPIQLGNAFYGQKGFTHDYDSIDEFYADLQK
                     "
BASE COUNT          108 a           45 c           57 g           98 t
ORIGIN      
        1 tttaaatccc atccatggga aaggcaaaaa aataatgtgc gttccgcttt aacattagac
       61 aaaataaatg aacatattaa atctctttca ctggataaat catccagagt acttttgaca
      121 gagcatttta atttggaggg tttgattaaa caatcggatt acatagtgac tttatgctct
      181 cagtctgcaa tagaagccgc attttgggga atgaaaccta ttcagcttgg gaatgcattt
      241 tatggacaaa aaggatttac tcatgattat gatagtattg atgaatttta tgcggattta
      301 cagaaaaa
//
LOCUS       DAZGHF010000534          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZGHF010000534 DAZGHF010000000
VERSION     DAZGHF010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.539"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            23..>306
                     /locus_tag="ACHK3A_005453"
     CDS             23..>306
                     /locus_tag="ACHK3A_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO8743357.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLT"
BASE COUNT           88 a           61 c           86 g           71 t
ORIGIN      
        1 gcaggtttca caggagaacg tcatgagcag ttcgtttcag aatgagatcc cgaaggcaag
       61 ggtgaatctg aaattagacc tacatacagg tggtgggcag aagaaagtgg agcttccgct
      121 caaactgctg gttgccggtg atttcagtaa cggacaggaa gagcgcccgc tgtcagagcg
      181 cataaaaatg gatgttaata aaaataactt taatgctgtt ctggctgata tctcgccgtc
      241 agtacgcctg aatgtggaga acacacttgc cggtgatggt actgaagaga gcatcagcct
      301 gacttt
//
LOCUS       DAZGHF010000535          304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZGHF010000535 DAZGHF010000000
VERSION     DAZGHF010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.540"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>304
                     /locus_tag="ACHK3A_005454"
     CDS             <1..>304
                     /locus_tag="ACHK3A_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO8743358.1"
                     /translation="VDAIYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPS
                     GVNFDADENDPVKLLIVLAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILS"
BASE COUNT           79 a           76 c           73 g           76 t
ORIGIN      
        1 tgttgatgcg atctatcact cgcacgacac tatcggtccg tactatgtgg ttggccctgg
       61 tattgcgatg cctcatgcgc gcccggaaga gggggctaac aagctgtcat tagcactgac
      121 cctgatccct tcaggtgtga attttgatgc cgatgaaaac gatccagtga aattactgat
      181 tgtgctggct gcaaccgaca gtaccagcca cattgaagcg atttctcaac tcgccaagtt
      241 attcgataac gaaaaagaca tacaggccat tttgacggcc aaaacgacgc aggacatttt
      301 gtct
//
LOCUS       DAZGHF010000536          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZGHF010000536 DAZGHF010000000
VERSION     DAZGHF010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.541"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>303
                     /locus_tag="ACHK3A_005455"
     CDS             <1..>303
                     /locus_tag="ACHK3A_005455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743359.1"
                     /translation="KQAAQSAVDKLQAGNDKSGLQGRLDGLTPAAVPAVTDKDGNGKA
                     DTADKALADAEAAVRAAEAKKDAADDAAAKADKDGNKLITPAEAKAVTDANAELATA"
BASE COUNT           79 a           71 c          119 g           34 t
ORIGIN      
        1 aaacaggcgg cacagtcagc ggtggataaa ctgcaggcgg gtaacgacaa gagcggtctg
       61 cagggacgtc ttgatggtct gaccccggcg gcggttccgg cggtgacgga taaggacggc
      121 aacggtaagg cggacacggc agacaaggct ctggcggatg cggaagccgc agtccgggcg
      181 gcggaagcga agaaagatgc agcagatgat gctgcagcga aagcagacaa ggacggtaac
      241 aagctgatta ccccggcgga agcgaaggcg gtgacggatg cgaatgcgga gctggcgaca
      301 gcg
//
LOCUS       DAZGHF010000537          298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZGHF010000537 DAZGHF010000000
VERSION     DAZGHF010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.542"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>298)
                     /locus_tag="ACHK3A_005456"
     CDS             complement(<1..>298)
                     /locus_tag="ACHK3A_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743360.1"
                     /translation="LPPVEWLTYGSGYLAGMKLGGTPLLEYMRDRLHRETVRSFGSMA
                     GNTAAYELTSTYTPAGQLQSRHLNSLVFDCDYNWNDNGDLMRISGPRQTREYGY"
BASE COUNT           47 a          100 c           85 g           66 t
ORIGIN      
        1 ctgtagccgt attcccgcgt ctgtcgcggg ccgctgatgc gcatcaggtc gccgttgtca
       61 ttccagttat agtcacaatc aaacaccagg ctgttcaggt gccggctctg taactggcct
      121 gcgggggtgt aagtgctggt cagttcatat gcggctgtat tgcctgccat gctgccgaag
      181 ctgcgcaccg tctcccggtg cagtctgtcc cgcatgtact ccagcagcgg cgtcccgccc
      241 agcttcatgc ccgccagata accgctgcca taggtcagcc actccaccgg cggcaggc
//
LOCUS       DAZGHF010000538          296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZGHF010000538 DAZGHF010000000
VERSION     DAZGHF010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.543"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>296
                     /locus_tag="ACHK3A_005457"
     CDS             <1..>296
                     /locus_tag="ACHK3A_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743361.1"
                     /translation="RYHYDLTGKLTQSEDEGLVILWYYDESDRITHRTVNGEPAEQWQ
                     YDGHGWLREISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHETKQ"
BASE COUNT           77 a           75 c           96 g           48 t
ORIGIN      
        1 aacgttatca ttatgacctg accggaaaac tcacgcagag tgaagatgag gggcttgtca
       61 tcctctggta ctacgatgaa tcggaccgta tcactcaccg cacggtgaac ggcgaaccgg
      121 cagagcagtg gcagtatgat ggccacggct ggctgagaga aatcagccac ctgagcgaag
      181 gccaccgtgt tgccgtccac tatggctatg acgataaggg ccgtctgacc ggggagcgcc
      241 agacggtgga gaacccggag acgggggaac tgctgtggca gcatgagacg aaacag
//
LOCUS       DAZGHF010000539          293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZGHF010000539 DAZGHF010000000
VERSION     DAZGHF010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.544"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>293
                     /locus_tag="ACHK3A_005458"
     CDS             <1..>293
                     /locus_tag="ACHK3A_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 family protein"
                     /protein_id="HFO8743362.1"
                     /translation="RISAIQDGVAAPGVMVVPRLWGNEPGVLAGRLCNPSVTVADSPA
                     RVATGALVAMGNDEIPQDGKKQPLELATLRSLEGKRFSVPMWYHDFDGLYWSDG"
BASE COUNT           53 a           86 c           98 g           56 t
ORIGIN      
        1 cgcatcagtg ccatacagga cggcgtggca gcacctggcg tgatggtggt tccccgctta
       61 tggggaaatg agcccggcgt actggcgggc cgtctctgta atccttccgt caccgttgcg
      121 gacagtccgg cacgtgtggc aaccggtgcc ctggtggcga tgggtaacga tgagatcccg
      181 caggacggca agaaacagcc ccttgagctg gccacgctgc gctcgctgga aggcaaacgc
      241 ttcagcgtgc ccatgtggta tcacgatttc gacgggctct actggtcaga cgg
//
LOCUS       DAZGHF010000540          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZGHF010000540 DAZGHF010000000
VERSION     DAZGHF010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.545"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            122..>287
                     /locus_tag="ACHK3A_005459"
     CDS             122..>287
                     /locus_tag="ACHK3A_005459"
                     /inference="COORDINATES: protein motif:HMM:NF022218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 family protein"
                     /protein_id="HFO8743363.1"
                     /translation="MSWPNVTVSHQNRFNGATREVERTLLFVGYGKKNTGNTLSVSPE
                     TDLDDVLGPDE"
BASE COUNT           74 a           78 c           80 g           55 t
ORIGIN      
        1 cgtcgcgcgt ctttctgggg gtaacaaacg aagaattttc acacatcctg gcacgccaga
       61 tgaacgcaat gggctacggc ctggacgttg acgcacagga tatcagataa ggagtcacac
      121 cgtgagctgg ccgaatgtaa ccgtaagcca tcagaaccgt ttcaacggtg ccacccgtga
      181 agtggagcgc acgctgcttt ttgtgggcta tggcaaaaag aacacaggta acaccctttc
      241 cgtctcccct gagaccgacc tggacgacgt gctgggaccg gatgaaa
//
LOCUS       DAZGHF010000541          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZGHF010000541 DAZGHF010000000
VERSION     DAZGHF010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.546"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            11..>287
                     /locus_tag="ACHK3A_005460"
     CDS             11..>287
                     /locus_tag="ACHK3A_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO8743364.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYY"
BASE COUNT           66 a           65 c           94 g           62 t
ORIGIN      
        1 gatgagaatg atgagtgata tgtcgcatca ggaacgtctg atcaggcggg tcaggaaact
       61 gctggagctg agccgtaata acagcaatgc ccatgaggcc gggctggcgc ttgcaagggc
      121 gcaacagctg atggcgaaac acggtatcac ggagcaggat gccggacttt cttcggtgca
      181 gacctcttca tcacaggggg caccttctga tgctgaaaaa gttccggcgt ggatgtttcg
      241 tctggtcatg gtggtgacac agacatttgg ctgccgggcg tattacg
//
LOCUS       DAZGHF010000542          286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZGHF010000542 DAZGHF010000000
VERSION     DAZGHF010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.547"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(165..278)
                     /locus_tag="ACHK3A_005461"
     CDS             complement(165..278)
                     /locus_tag="ACHK3A_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8743365.1"
                     /translation="MICITVVLIIWITHSSLCEFRFRIAGAEIAAFLQCKQ"
BASE COUNT           65 a           84 c           69 g           68 t
ORIGIN      
        1 cacccgatat tctgactgtc atggatttcc cccataaaaa aaccgcagta ccggtttccc
       61 ggcctgcggt aaaatttgtg gtttgttggt gttaaaacgg tgccatccgg ctgaccaccc
      121 tcagcaaccg gtcggcgggg gatattctcc cccgccacgg tttcttactg cttacactgt
      181 aagaacgccg caatctccgc gcccgctatc cggaaccgga actcgcacag tgaactgtgg
      241 gtgatccaga taatgagcac taccgtgata caaatcacgg tggttt
//
LOCUS       DAZGHF010000543          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZGHF010000543 DAZGHF010000000
VERSION     DAZGHF010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.548"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT           79 a           55 c           72 g           78 t
ORIGIN      
        1 gaggtccggc tgacggaagt ttctgacgtg tggctctgtt gttccctgaa tgtctttctg
       61 tacgtatctg agcatgttac tgacaaaggt tattattagc ttatcgatcg atataaacga
      121 tcgataaaat aggtatcgat cgttttattc tattgtttgg ctattgcacg gacaagagag
      181 acagtaacga aaacagcgct tttgtgagca gactcagagt cagggagggc atcagtcacg
      241 acaggttcag aagaaaatcc agttccatac cgcacgggca acag
//
LOCUS       DAZGHF010000544          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZGHF010000544 DAZGHF010000000
VERSION     DAZGHF010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.549"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>284
                     /locus_tag="ACHK3A_005462"
     CDS             <1..>284
                     /locus_tag="ACHK3A_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFO8743366.1"
                     /translation="EAVGYYGSKWRNDQKTWTSVDRASHFSSWARRGIISDRLATAIL
                     VHAGRTAEFITGKQPDTVKPTGPSGEGPEREPEAPVSNRTLMSLLASGED"
BASE COUNT           65 a           75 c           89 g           55 t
ORIGIN      
        1 tggaagccgt cggttattac ggttcaaagt ggcgtaatga ccagaagacc tggacatctg
       61 tggacagggc cagccatttc agttcatggg ctcgccgtgg gattatttcc gaccgtctgg
      121 caacggcgat tctggtgcat gccgggagaa ccgctgaatt cattaccgga aaacagcctg
      181 atacggtgaa gcccaccgga ccttccggtg aaggcccgga gagagagccg gaagccccgg
      241 tcagtaaccg aaccctgatg agtctgctgg cgtccggtga agac
//
LOCUS       DAZGHF010000545          283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZGHF010000545 DAZGHF010000000
VERSION     DAZGHF010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.550"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..244
                     /locus_tag="ACHK3A_005463"
     CDS             <1..244
                     /locus_tag="ACHK3A_005463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743367.1"
                     /translation="RNTSPRRAAAMISKYTTNARKPGKDARHHIAVLRNLLLEEIDDF
                     TATLSGPGAPVTPGEIHRDLKRRIENAIDYTLNPTE"
BASE COUNT           87 a           89 c           67 g           40 t
ORIGIN      
        1 cagaaacaca agcccacgga gagctgcggc catgatcagc aagtacacca ccaacgcacg
       61 taaacccggt aaagacgcac gccaccacat cgccgtactc cgcaatctgt tactggagga
      121 aatcgacgac ttcacggcaa cgctgagcgg tccgggcgca ccagtaacac caggtgagat
      181 ccaccgggac cttaaacgcc gtattgaaaa cgccattgat tacaccctga acccgaccga
      241 ataaccggaa cagtaacgga ggttatcgcg atgagactta cac
//
LOCUS       DAZGHF010000546          282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZGHF010000546 DAZGHF010000000
VERSION     DAZGHF010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.551"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>282)
                     /locus_tag="ACHK3A_005464"
     CDS             complement(<1..>282)
                     /locus_tag="ACHK3A_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743368.1"
                     /translation="LYSRIYGVEWPSGNNVRHARDEMSSLVLLIDTPWYPPSGELVGE
                     ISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDLQAEWPGRETLPQPR"
BASE COUNT           58 a           92 c           68 g           64 t
ORIGIN      
        1 tcggggctgt ggaagtgttt ccctgcccgg ccattcggcc tgcagatcac tgtccgtatg
       61 ctctccgcca tcgtaacagt tgtaaccctg tataccgtgt accggctcgc tgtaccagtg
      121 tcggacctcg cagtcaaaca ccttcgagat ctcaccaacc agttcgcctg aaggcggata
      181 ccagggcgtg tcgatcagca gcaccagact gctcatctca tcacgcgcat ggcgaacatt
      241 atttcctgac ggccactcca caccgtatat ccggctgtaa ag
//
LOCUS       DAZGHF010000547          279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZGHF010000547 DAZGHF010000000
VERSION     DAZGHF010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.552"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT           75 a           96 c           58 g           50 t
ORIGIN      
        1 acagctgcat cgtcgcattc cagacgaacc tgcaccctgt cagcccgacc gtttacagta
       61 aatacctgca ctgaacttat gcgatagttc agcatataca ctctccctct cagctcctca
      121 ggaatacccc ggaaccgtca gtcatgccac cggggagaaa agcaggcagc gaatcatgac
      181 cgcatacaga acgctgcccg ccccggtgac tcagacccgg gttcagaact cccagttgac
      241 acccacattc caggaaacat caccgtcact gaacgcagt
//
LOCUS       DAZGHF010000548          279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZGHF010000548 DAZGHF010000000
VERSION     DAZGHF010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.553"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..111
                     /locus_tag="ACHK3A_005465"
     CDS             <1..111
                     /locus_tag="ACHK3A_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFO8743369.1"
                     /translation="AGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            116..>279
                     /locus_tag="ACHK3A_005466"
     CDS             116..>279
                     /locus_tag="ACHK3A_005466"
                     /inference="COORDINATES: protein motif:HMM:NF016437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFO8743370.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPL"
BASE COUNT           57 a           83 c           79 g           60 t
ORIGIN      
        1 gccggaaaga ccacgaaaaa aatcaagttc atggtcacaa gcccggactt tatcagcatt
       61 gacggcgtgc cctacctgtc agcccgtgac acccgcgatc tgaaggggtg atgccatgcc
      121 ggacggtcag acttcactcc tggcaaaact gattatcatc ggtgctgcca tcgggctggg
      181 gcgtctcatg gtgagccgcg atcgcatcac agcccgtctg cttatcgggc gaatgattat
      241 gggctctgcc gtggcaccgg ttgccgccat tccgctgtt
//
LOCUS       DAZGHF010000549          277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZGHF010000549 DAZGHF010000000
VERSION     DAZGHF010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.554"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT           84 a           73 c           70 g           50 t
ORIGIN      
        1 atgtaaaaac cgcacaggaa atggctctgg aagtgggatg cagtgagtgc acagtgaggt
       61 acacactgcg taaacggggt tacaacctga aaaaatgcgg tgagaaccac cattgcgcca
      121 gatacagcga tcatctggtt gagctggtca cggagctacg cgacatacac aacatgacgt
      181 tctgcatgat cgctgaacac atcaacagca cgatacagat gcacattaac gacgatgcgg
      241 cgtttcatct ctaccaccgc cgcaccgcaa ctgacgc
//
LOCUS       DAZGHF010000550          269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZGHF010000550 DAZGHF010000000
VERSION     DAZGHF010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.555"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            76..>269
                     /locus_tag="ACHK3A_005467"
     CDS             76..>269
                     /locus_tag="ACHK3A_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8743371.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARH"
BASE COUNT           67 a           65 c           71 g           66 t
ORIGIN      
        1 gatcccaaaa ttccttatca gcatccgtcg ctcatgaatg ccagtcatga atcgtgatgt
       61 tctgccggag gctgtatgtc ccctgattac gttcagatgg agatgcctgc caggtacagc
      121 caggcagatt cagagtggat taagcggcag ttacagagcc tgccttcctc actgagacag
      181 aaggtcgccc tgaaatacgc ggaggtttac gaagttactt ttgattccga gcctgtttca
      241 ttccgtaagg agaacagagc gaggcatga
//
LOCUS       DAZGHF010000551          267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZGHF010000551 DAZGHF010000000
VERSION     DAZGHF010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.556"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(10..>267)
                     /locus_tag="ACHK3A_005468"
     CDS             complement(10..>267)
                     /locus_tag="ACHK3A_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFO8743372.1"
                     /translation="VSIGQDVVHSIIESGIAALLIGERSPTLRSDIITRLILLVEDDE
                     RLVPGTVFINEEAPGRLHVTAETQDFGRINTRVNYERETGR"
BASE COUNT           51 a           83 c           67 g           66 t
ORIGIN      
        1 tgattaaaat caacgtccgg tttccctttc atagttcacc ctggtgttta tgcgtccaaa
       61 atcctgtgtc tctgccgtca catgcagcct gcccggtgcc tcttcgttaa tgaacaccgt
      121 ccccggaacc agccgttcat catcctcaac caggaggata agccgggtaa tgatgtcgct
      181 gcgcagcgtc gggctgcgtt cgccgataag cagggcggcg atcccgcttt cgataatgct
      241 gtgcaccacg tcctggccga tgctgac
//
LOCUS       DAZGHF010000552          262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZGHF010000552 DAZGHF010000000
VERSION     DAZGHF010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.557"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>262
                     /locus_tag="ACHK3A_005469"
     CDS             <1..>262
                     /locus_tag="ACHK3A_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8743373.1"
                     /translation="LREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIA
                     ENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDP"
BASE COUNT           61 a           64 c           83 g           54 t
ORIGIN      
        1 ggttgcgtga aaagatgaag accctgtcgc gacactcaga gttggcgaag gcgttcgcgt
       61 acgcacttaa ccagtggccg gcactgacgt actatgcgaa cgatggctgg gtggaaatcg
      121 acaacaacat cgctgaaaat gccctgcggg cggtcagtct gggtcgtaaa aacttcctgt
      181 tcttcggctc tgaccatggt ggtgagcggg gagcgctact gtacagcctg atcgggacgt
      241 gcaaactgaa tgacgtggat cc
//
LOCUS       DAZGHF010000553          261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZGHF010000553 DAZGHF010000000
VERSION     DAZGHF010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.558"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT           72 a           46 c           64 g           79 t
ORIGIN      
        1 tatcacgtga gagctgcaca ttattggagg ggcaggggaa aacaccattt tgggaacaat
       61 gaatcaggta ctcttaccac tgaagcaaga tcatccgggc accaggtggt ggtgcagtgc
      121 tttggaaatg gtcccctcgg gggagttgtc attattttct atgctatttg cttatgatat
      181 tcatcaggtg aatttaataa attcctaatt ttaaatatcc actattaagc cagtgtttaa
      241 cggcgttaag atggcaatgt g
//
LOCUS       DAZGHF010000554          261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZGHF010000554 DAZGHF010000000
VERSION     DAZGHF010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.denovo.559"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..65)
                     /locus_tag="ACHK3A_005470"
     CDS             complement(<1..65)
                     /locus_tag="ACHK3A_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO8743374.1"
                     /translation="MAIPVYLWLQDDGGADIKGSV"
     gene            complement(100..>261)
                     /locus_tag="ACHK3A_005471"
     CDS             complement(100..>261)
                     /locus_tag="ACHK3A_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8743375.1"
                     /translation="DIPQSCFAVQGYGESRPVAPNETAEGRARNRRVEISLVPQADAC
                     RLPDITRAP"
BASE COUNT           55 a           87 c           62 g           57 t
ORIGIN      
        1 tccacggagc ctttaatatc cgctccgcca tcatcctgca gccacagata aacaggaatt
       61 gccataaagt tactccctct cgttgtctta cgcgccctgt tacggcgccc gggtgatgtc
      121 cggcagacga caggcatcgg cctgcggtac cagactgatt tcaacacggc ggttacgcgc
      181 tcgcccttcc gccgtctcat tcggagccac gggacgactc tcaccatatc cctgtacggc
      241 aaaacagctt tgagggatat c
//
LOCUS       DAZGHF010000555        15649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.guided.109, whole genome shotgun sequence.
ACCESSION   DAZGHF010000555 DAZGHF010000000
VERSION     DAZGHF010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.guided.109"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHK3A_005472"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHK3A_005472"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO8743376.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHK3A_005473"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHK3A_005473"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO8743377.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHK3A_005474"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHK3A_005474"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO8743378.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDANPLVDDNKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHK3A_005475"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHK3A_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO8743379.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLLGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6384..6698)
                     /gene="yjeO"
                     /locus_tag="ACHK3A_005476"
     CDS             complement(6384..6698)
                     /gene="yjeO"
                     /locus_tag="ACHK3A_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO8743380.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6695..7009)
                     /gene="yjeN"
                     /locus_tag="ACHK3A_005477"
     CDS             complement(6695..7009)
                     /gene="yjeN"
                     /locus_tag="ACHK3A_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO8743381.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            complement(7072..8574)
                     /gene="yjeM"
                     /locus_tag="ACHK3A_005478"
     CDS             complement(7072..8574)
                     /gene="yjeM"
                     /locus_tag="ACHK3A_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO8743382.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8793..9770)
                     /gene="epmA"
                     /locus_tag="ACHK3A_005479"
     CDS             complement(8793..9770)
                     /gene="epmA"
                     /locus_tag="ACHK3A_005479"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO8743383.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10095..11903
                     /gene="frdA"
                     /locus_tag="ACHK3A_005480"
     CDS             10095..11903
                     /gene="frdA"
                     /locus_tag="ACHK3A_005480"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO8743384.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11896..12630
                     /gene="frdB"
                     /locus_tag="ACHK3A_005481"
     CDS             11896..12630
                     /gene="frdB"
                     /locus_tag="ACHK3A_005481"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO8743385.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12641..13036
                     /gene="frdC"
                     /locus_tag="ACHK3A_005482"
     CDS             12641..13036
                     /gene="frdC"
                     /locus_tag="ACHK3A_005482"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO8743386.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            13047..13406
                     /gene="frdD"
                     /locus_tag="ACHK3A_005483"
     CDS             13047..13406
                     /gene="frdD"
                     /locus_tag="ACHK3A_005483"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO8743387.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            13469..14602
                     /gene="ampC"
                     /locus_tag="ACHK3A_005484"
     CDS             13469..14602
                     /gene="ampC"
                     /locus_tag="ACHK3A_005484"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO8743388.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            14691..15224
                     /gene="blc"
                     /locus_tag="ACHK3A_005485"
     CDS             14691..15224
                     /gene="blc"
                     /locus_tag="ACHK3A_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO8743389.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(15225..15542)
                     /gene="sugE"
                     /locus_tag="ACHK3A_005486"
     CDS             complement(15225..15542)
                     /gene="sugE"
                     /locus_tag="ACHK3A_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO8743390.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT         3896 a         4153 c         4041 g         3559 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagttct gccaccgact
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcata tatttaaaca ggaagcgacg atcctggccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacgcgctc caccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcagactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgttgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccagc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgcccg gatttttacg acggtgtgaa acctattgct gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcattcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagtcata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgttggcaaa tccagcctgc
     1561 tgaacgcatt gctgggtctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 gtctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgactc cccaggagtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactact taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaaggaaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gatatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggttatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggctggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccac
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttttccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggggcgac gattgtaggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacaccaga cgctgaaccg gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccaacccatt ggttgacgac aacaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atctcacaag
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccgga agtcgtagag gcgctccagt
     3181 ccgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattacccg aaactttccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgaacc gcgcagcgtg tcaccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggaacgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgat gcccgccgtc
     3481 agttaaatga aatcgagcgc cgcctgggga cgcttaccgg caattccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtc gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgtc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaaca
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gctcgtccaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggcta ccggaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct aaataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtgga gacacgctac tactggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaatgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgta atgatgctga
     4441 ccagtaaaga gacgattttg ccgctgcttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgcctgt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggtgat ggcgtaacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgggtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt cactctcagt ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagaag gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcaccgctgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc gcaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca gcgtgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcact tccacggtac agggcgtaga aagcctggag ccgattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattctgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agtgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg caaagaaatt atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtaa tcgataaccc agcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt caaattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc actacgccat gagattcacc agctaattct ggcaggattc catgcccacg
     6241 gtattgatat gccattcccg cccttccaga tgcgtttgga aagcctgaat ggtaagcaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taaggtcttt
     6361 gtggccggat gaacccatcc ggcttataag caaaactgat agcgcagaaa aaactgccac
     6421 agccaatagc cgaaaatagc cagcgcggtg gcgtacataa accagccgcg tttttttcgc
     6481 cacaaagcga accagagaaa agggaaaaag agcggtaacg tcattatcac accgacatca
     6541 cgggtatcgt catcttcgag atacatcagc acatcgcaga cgttattaat cccgcgtcca
     6601 tcaatcatcc actctttgtc cagcgcggaa gtgatggtta tccagaggaa cgccacaata
     6661 aaccagatgc agcacaagat aaacatgcgc gcactcatga gataatcggt cgcttaattt
     6721 cgctatagcg catacgaaac aaatcgccgt atcccactaa ttctccgcga aaaaccagac
     6781 atctaataag ataagcgcaa tagatatccg gcaggtttcc ccaccgcgga ccatcgcgct
     6841 ctttgagggt attaatagtg ttacccacca ccatcgctac tttgcgggtg tggcgggtat
     6901 cgacaaaaga gaggaccgtt tgctctattt ccgccacatc cccggcagaa aattgcagct
     6961 ccctgatttc atcttcggtt atcactggat cccgactagc gtcgtccata acgtcttcct
     7021 ttccagacaa ctcatcgccg gagagacgat gagttgcagg atttcatatg atcagtcgag
     7081 agctaactca ggtttgttcg ccatgcgact gcaatagttc tggtaaatcg ccatcgctaa
     7141 cagcgagaag aagacagggc cgccaatcat ccacaatgtg ctgtcccagt ctccagcttc
     7201 aaccacaggt tgaatgatgg tgaagacgtt cgcaaatgtc accaccagta cgacaaccac
     7261 tgttgcaatc attgccgaca tatgcgtttt gaaaatcaca aacggtctgt cgagatcctg
     7321 acgtgcttta aagaatggga aagccagcgc gaggaacagg taaggaagcg tcatagacac
     7381 gttcgccatc agcgtcagct tgttaaagaa cgccgatgcg gtaccgccac caaacgaaac
     7441 cagcaggatg aagacagtaa ccaacccgca ctgcatccac atggcgatag acggcatccc
     7501 catcgcattc aggcgcgtca tcggttccgg ccacaacgct ttcggcgtcc cctggatgat
     7561 ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg gtataagcga ggaacatcga
     7621 aagtccggta atacgcgcaa accatacgcc cagcgacaat gacgcttcag gtgacaaatg
     7681 cagtgcgtta cccagcgtca ccccgaggct cttcatcagc acataggtaa tattgccgag
     7741 gttaacggaa ccattactta atacctgctg ccagtttgtg ctgacgcccc ataaaaatat
     7801 tgccagcgaa taaccgattg aaataacaat agcggcaaaa acaatacctt tggcaaagtt
     7861 cttttctgga ttttccgttt tatcgaccag accaccaacc gcttcaattc caccataggc
     7921 aaaaatagca aataccacaa acgatagcat tgccagaccg gactgataac ctgggttcgg
     7981 tgatgcaagg aaattaatat cctgcgcgaa atgcccacca tttaataaca aaatagtaat
     8041 gcttactaac agcaatacta aattcagaca cattactgca ataccgccca ccgcagtaat
     8101 gcgggcaatt ttattaatcc cttttgaagc aacgacggtg accagaatca tccatgccac
     8161 tgccagcaga ccaaccacct gcgtaggctc cagtccggca atacgccagt gctgggtcat
     8221 gtcgctacca tagaggaatg ttgagaacgg tacccaaact ttcgcggagg tactcaccat
     8281 ccagatgata taagaggaaa accacataaa cgtaccaatg aaggcaaaac gtggtccgac
     8341 actattattc atccaggaat agataccgcc ttcttctttg cgataagcag ctcccatttc
     8401 agccatcatt aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat
     8461 cgcactataa cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt
     8521 aaagatcatc aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaaac
     8581 atccttcatt tcagcgcgtg tataaaacgc gcccactgaa ctgcactgcc caacattgag
     8641 cgggcagcgt aaaaaccaat acagaattga tacctggatg atatcaaata tccagtctta
     8701 aattgtggcc tttagcggaa aaatgaaaac atatgctaca gaatggcgct tatcacgcca
     8761 ttcttcgcgg ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg
     8821 acttcagcca gtgtctccgc gcccagcgcc aacatcacca gacgatcaac gcctaatgcc
     8881 acgccggaac agtcaggcat accgactttc aaggcttcaa tcagattctg gtcaatgggg
     8941 tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc
     9001 tcacgggcat ccgtcaattc atggaaacca ttcgccagct caataccttt ataataaacc
     9061 tcaaagcgtt cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg
     9121 gctggaaagt ggtacacaaa ggtcggtttt tctttgccaa tatttggctc tacgccaaag
     9181 gtaaacagca attgtagaag cgtgtcgcgg tcttcttcag tatcggcaac gttgctcaaa
     9241 tccagtttcg ctgccacttc ccgcagttgc gttttgtcgg cagagagtgg gtcaatttcc
     9301 agataacgca agaaagcttg ttgataagaa aggctttctg ctgccggaca gtccagcacc
     9361 tgttgcaaga gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac
     9421 cactcaagca tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg
     9481 cggcacagct ggaataccgg cccacaacca gcaaccagca ggcgtttcat atggtattcc
     9541 gggctggtca ttaaccagag attcatcccc tgcgaatgcc cggggcctac gaaacgtgtc
     9601 tcaaacggga ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc
     9661 tccagcactc cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcgcgtttt
     9721 aataagttag gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct
     9781 ccagtttttg acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc
     9841 aggaatcaaa caagggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta
     9901 aattaatcat cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca
     9961 aatttcagac ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg
    10021 ttcgcagacc gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa
    10081 tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg
    10141 cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa
    10201 tctcaaaagt atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg
    10261 tcgcgcagga tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact
    10321 ggttgtgtga gcaggatgtc gtggattatt tcgtccacca ctgccccacc gaaatgaccc
    10381 aactggaact gtggggatgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc
    10441 gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata
    10501 tgctgcacac gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac
    10561 atttcgtact ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaatgaaca
    10621 tgatggaagg cacgctggtg cagatccgtg ctaacgcggt cgttatggct accggcggtg
    10681 cgggtcgcgt ttatcgttac aataccaatg gcggcatcgt taccggtgac ggtatgggta
    10741 tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg
    10801 gtctgccagg ttccggtatc ctgatgaccg aaggctgccg cggtgaaggc ggtattctgg
    10861 tcaacaaaaa tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg
    10921 gcgagccgaa aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct
    10981 ggcacgaatg gcgtaaaggc aacactatct ccacgccgcg tggcgatgtg gtttatctcg
    11041 acttgcgtca cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg
    11101 cgaaagcgta cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgaccgcac
    11161 actacaccat gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt
    11221 tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg ggttctaact
    11281 ccctggcgga actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag
    11341 caactgccgg taatggcaac gaagcggtaa ttgaagcgca ggcagctggc gttgaacaac
    11401 gtttgaaaga tctggttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa
    11461 tgggcctggc tatggaagaa ggctgcggta tctaccgtac gccggaactg atgcagaaaa
    11521 ccatcgacaa gctggcagag ctgcaggaac gcttcaaacg cgtgcgcatc accgacactt
    11581 ccagcgtgtt caacaccgac ctgctctaca ccattgaact gggccacggt ctgaacgttg
    11641 ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc
    11701 tggacgaagg ttgcaccgag cgtgacgacg tcaacttcct caaacacacc ctcgccttcc
    11761 gcgatgctga tggcacgact cgcctggagt acagcgacgt gaagattact acgctgccgc
    11821 cagctaaacg cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata
    11881 agaaggagaa ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac
    11941 ccggaagtcg ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc
    12001 tcattactgg atgcgctggg ctacatcaaa gacaacctgg caccggacct gagctaccgc
    12061 tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa
    12121 ctggcatgta aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct
    12181 aacttcccga ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa
    12241 gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag
    12301 accccggcgc agatggccaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc
    12361 tacgccgcgt gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg
    12421 ctggcgcatc gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag
    12481 ttgaacagcc agaacggcgt atggagctgt actttcgtgg gctactgctc cgaagtctgc
    12541 ccgaaacacg tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac
    12601 tttcttatcg cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc
    12661 gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttctacat
    12721 gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct
    12781 gtttgccctg aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc
    12841 ggttatcgtg atcattaacc tgatcactct ggcggcagcc ctgctgcaca ccaaaacctg
    12901 gtttgagctg gcaccaaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga
    12961 gccaattatc aaaagcctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt
    13021 tgccctgtac tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac
    13081 cggtattctg gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga
    13141 tgatcctgct ggttggtatt ctgctgccac tggggctgtt tccgggtgac gcactgagct
    13201 acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga
    13261 tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa
    13321 tccacgtacc tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca
    13381 cgctgattgg tgtcgttaca atctaacgca tcgccaatgt aaatccggcc cgcctatggc
    13441 gggccgtttt gtatggaaac cagaccctat gttcaaaatg acgctctgcg ccttattaat
    13501 taccgcctct tgctccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac
    13561 aattaccccg cttatagagc aacaaaagat ccccggtata gcggtggcgg taatttatca
    13621 gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac
    13681 acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg
    13741 cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcgacaaaat actggcctga
    13801 acttaccgct aaacaatgga atgggatcac actattacat ctcgcgacct acaccgctgg
    13861 cggcctgcca ttgcaggtgc cggatgacgt taaatcctca agcgacttgc tgcgcttcta
    13921 tcaaaactgg caacctgcat gggctccagg aacacaacgt ctgtatgcca acgccagtat
    13981 tgggttgttc ggtgcactgg cggtgaaacc atccggtttg agctttgaac aggcgatgca
    14041 aactcgtgtt ttccagccac tcaaactcac tcatacgtgg attaatgtgc cgtccgcaga
    14101 agaaaagaat tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc
    14161 gttagatgct gaggcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca
    14221 aagcaattta aaaccccttg atatcactga gaaaacgctt caacaaggga tacaactggc
    14281 acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga
    14341 ctggccggtc aatcctgaca tcatcattaa cggcagtgac aataaaattg cactggcagc
    14401 acgccccgta aaagcgatta cgcccccaac tcctgcagta cgcgcatcat gggtacataa
    14461 aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg
    14521 tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca
    14581 gattctcaac gctctacagt aaaatttcat cgggtccgaa tttttggacc ttttctccgc
    14641 ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc
    14701 tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc
    14761 cgccccgtgg cgtgaccgta gtgaataatt tcgatgctaa acgctatctc ggtacctggt
    14821 atgagattgc ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat
    14881 acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag
    14941 aaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc
    15001 tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg
    15061 aataccgcca tgcgctggtt tgcggtccgg accgcgacta cctgtggata ctctcccgca
    15121 cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt
    15181 ttgatgtcag taagtttatt tgggtacagc agcccggtag ttagttagtg agtgctgagt
    15241 ttcagaccaa taatccccaa tacgattaac gccagactcg ccaggcgcat cgggttagcg
    15301 gactcgccga gcagcacaat gccggtgatg gccgcgccga ctgcgccaat acccgtccac
    15361 acggcataag ccgtccctac tggtaacgat ttcatcgccc aggcaagtag cgccatactg
    15421 acaatcatcg ccgtcacagt aataacactt ggcgtcaaac gactaaagcc gtgggtatat
    15481 ttcaggccaa cggcccatac cacttccagc aggccagcaa taactaagat aatccaggac
    15541 atatcaggct ccggagaatt ggggccgtcc ccggtgaaaa gaggcgtttg caggtcgtcc
    15601 tgcaaagctg atggttgaac ggatattttg ccaggtggca ggggaattt
//
LOCUS       DAZGHF010000556         4256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.guided.243, whole genome shotgun sequence.
ACCESSION   DAZGHF010000556 DAZGHF010000000
VERSION     DAZGHF010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.guided.243"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            120..326
                     /locus_tag="ACHK3A_005487"
                     /pseudo
     CDS             120..326
                     /locus_tag="ACHK3A_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            322..674
                     /gene="prfH"
                     /locus_tag="ACHK3A_005488"
                     /pseudo
     CDS             322..674
                     /gene="prfH"
                     /locus_tag="ACHK3A_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(731..2188)
                     /gene="pepD"
                     /locus_tag="ACHK3A_005489"
     CDS             complement(731..2188)
                     /gene="pepD"
                     /locus_tag="ACHK3A_005489"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO8743391.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2449..2907
                     /gene="gpt"
                     /locus_tag="ACHK3A_005490"
     CDS             2449..2907
                     /gene="gpt"
                     /locus_tag="ACHK3A_005490"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO8743392.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            2999..4243
                     /gene="frsA"
                     /locus_tag="ACHK3A_005491"
     CDS             2999..4243
                     /gene="frsA"
                     /locus_tag="ACHK3A_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO8743393.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
BASE COUNT         1015 a         1065 c         1121 g         1055 t
ORIGIN      
        1 tgcaggaagg cgacaagcga gtgaatcccc aggagcttac taaagtaagt gactggggtg
       61 aacgaacgcg gtcgcagtac atgcaacttg aagtatgacg agtataaatg gaatgataca
      121 tgggcaaata tattcgtccc ttatccgatg cggtatttac catcgcatct gatgacctgt
      181 ggatcgagag tttagcgatc caacaattac acaccacggc aaatttaccc aacatgcagc
      241 gcgttgtggg gatgccagat ttacatcccg gacgcggcta cccgattggc gcagcgttct
      301 tctctgttgg tcgtttttac ccggcaagac gtcgcggtaa cggtgctgga aacagaaacg
      361 ggccgctact ctgacacgct gcgttcggcg ctgatttctc tggatggcga taatgcatgg
      421 gcattaagcg aaagttggtg cggcactatt cagtgtattt gtccgagtcc gtatcggcct
      481 catcatgggc gcaaaaactg gtttctgggc atgctaacaa gcggctggcg cgattgctga
      541 ttgcctggaa gctggaacaa cagcaacagg aaaatagcgc ggcgctgaaa tcgcagcggc
      601 gaatgttcca tcaccagatt gaacgtggca acccgcgacg aacgttcaca gggatggctt
      661 ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca tccggcagca
      721 aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagtagt gtccagtaat
      781 gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg atggttggcc
      841 cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat tccaggcccg
      901 cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg ctgataggtt tcacgtacca
      961 gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct ttcgcttcgg
     1021 ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag tctttaccgc
     1081 tgtcgatcag agaacggatc aggcagtgaa tttctacgtt attgtcagtc atggtcacca
     1141 caccgacgtt cagggaggtt tcgaccacac cttttgccac gtcggagttg cggatcacac
     1201 cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca atcagggcag
     1261 ctttatcgtt cgcaacagag tccagcaaca aggccagatt tttctctttc tctgccagct
     1321 cgtttttcag gatctcctga taggtattca ccagagattt caaagcgtcg actttatcag
     1381 ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt gtgccgccgt
     1441 tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc cgccaggaag cgcaccagca
     1501 gtttgttggc attacccagg ccaacgtgga tttcaccgcc ggagtgaccg cctttcaggc
     1561 cttttaaggt taacttgaag gtttcaaagc cagctggaac cgcttcacga tctaaatgca
     1621 ggttggaggt gaagtcgata cctcccgcac aacccatgta gatttcacct tcttcttcgg
     1681 agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagcca aaagcaccgt
     1741 ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt
     1801 cgtcagccag aaccgccagt gcagaggcca taccaatgcc gttatccgca cccagcgtgg
     1861 tgccgcgtgc tttaacccat tcgccatcaa tataaggctg gataggatct ttggtgaagt
     1921 catgcacggt gtcgttattt ttttgcggca ccatatcgag gtgggcctgc aagacgaccg
     1981 gtttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta cctacctgat
     2041 cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct
     2101 cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct
     2161 gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca
     2221 gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc ttgcgagata
     2281 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
     2341 tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc
     2401 ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa
     2461 tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg
     2521 ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggtgcg
     2581 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
     2641 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
     2701 atcgttattg atgacctggt agataccggt ggtactgcgg ttgcgattcg tgaaatgtat
     2761 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgat
     2821 tatgttgttg atatcccgca aaatacctgg atcgaacagc cgtgggatat gggcgtcgta
     2881 ttcgtgccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     2941 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     3001 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     3061 gacgctagtc cgccgcttta gtcacggcgc acaactgcct gtgcagtcgg cccttgatgg
     3121 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     3181 tgacccccgt gaaatcctcg acgtccaggc gcgtattgtg atgagcgatg ccgaacgtac
     3241 cgacgatgat ttatacgata cggtgattgg ctaccgcggc ggcaactgga tttatgagtg
     3301 ggctacccag gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc cgcaactcag
     3361 cggtcgatac tggctgcatg cggctacgtt gtacaacatt gctgcctatc ctcatctgaa
     3421 aggagatgac ctggccgagc aagcgcaggc tttgtcaaac cgcgcctatg aagaggccgc
     3481 tcagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg gtgcgcccat
     3541 caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg
     3601 tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt ttgcgccgcg
     3661 cggcattgct atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct
     3721 cacccaggac tccagcctgt tgcatcagca cgttttaaag gcgctgccta acgtaccgtg
     3781 ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg
     3841 tctggcatac cttgaatcgc cgcgtctgaa agtggttgcc tgtcttggtc cggtagttca
     3901 taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc ttgacgttct
     3961 ggccagtcgt ttagggatgc atgatgcttc cgatgaagcg ttgcgagtgg agttgaatcg
     4021 ctattcatta aaagtgcaag gattgctggg acgtcgctgc ccaacgccaa tgttatcagg
     4081 ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca cgtcgtcatc
     4141 tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt ttgacaaagg
     4201 ccttcaggaa atcacagact ggatcgaaaa acgtttgtgt taaaaatttg ctaaat
//
LOCUS       DAZGHF010000557         2003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.guided.305, whole genome shotgun sequence.
ACCESSION   DAZGHF010000557 DAZGHF010000000
VERSION     DAZGHF010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.guided.305"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            259..1206
                     /gene="stxA1a"
                     /locus_tag="ACHK3A_005492"
     CDS             259..1206
                     /gene="stxA1a"
                     /locus_tag="ACHK3A_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO8743394.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1216..1485
                     /gene="stxB1a"
                     /locus_tag="ACHK3A_005493"
     CDS             1216..1485
                     /gene="stxB1a"
                     /locus_tag="ACHK3A_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO8743395.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          537 a          362 c          488 g          616 t
ORIGIN      
        1 ttgccagcct cccccagtgg ctggcttttt tatgtccgta acatcctgtg tatcaataaa
       61 tgttgttgtc tacgtacgtc aagtagtcgc atgagatctg accagatatg ttaaggttgc
      121 agctctcttt gaatataatt atcattttca ttacgttatt gttacgttta tccggtgcgc
      181 cgtaaaacgc cgtccttcag ggggtggagg atgtcaagaa tatagttatc gtatggtgct
      241 caaggagtat tgtgtaatat gaaaataatt atttttagag tgctaacttt tttctttgtt
      301 atcttttcag ttaatgtggt tgcgaaggaa tttaccttag acttctcgac tgcaaagacg
      361 tatgtagatt cgctgaatgt cattcgctct gcaataggta ctccattaca gactatttca
      421 tcaggaggta cgtctttact gatgattgat agtggcacag gggataattt gtttgcagtt
      481 gatgtcagag ggatagatcc agaggaaggg cggtttaata atctacggct tattgttgaa
      541 cgaaataatt tatatgtgac aggatttgtt aacaggacaa ataatgtttt ttatcgcttt
      601 gctgattttt cacatgttac ctttccaggt acaacagcgg ttacattgtc tggtgacagt
      661 agctatacca cgttacagcg tgttgcaggg atcagtcgta cggggatgca gataaatcgc
      721 cattcgttga ctacttctta tctggattta atgtcgcata gtggaacctc actgacgcag
      781 tctgtggcaa gagcgatgtt acggtttgtt actgtgacag ctgaagcttt acgttttcgg
      841 caaatacaga ggggatttcg tacaacactg gatgatctca gtgggcgttc ttatgtaatg
      901 actgctgaag atgttgatct tacattgaac tggggaaggt tgagtagtgt cctgcctgac
      961 tatcatggac aagactctgt tcgtgtagga agaatttctt ttggaagcat taatgcaatt
     1021 ctgggaagcg tggcattaat actgaattgt catcatcatg catcgcgagt tgccagaatg
     1081 gcatctgatg agtttccttc tatgtgtccg gcagatggaa gagtccgtgg gattacgcac
     1141 aataaaatat tgtgggattc atccactctg ggggcaattc tgatgcgcag aactattagc
     1201 agttgagggg gtaaaatgaa aaaaacatta ttaatagctg catcgctttc atttttttca
     1261 gcaagtgcgc tggcgacgcc tgattgtgta actggaaagg tggagtatac aaaatataat
     1321 gatgacgata cctttacagt taaagtgggt gataaagaat tatttaccaa cagatggaat
     1381 cttcagtctc ttcttctcag tgcgcaaatt acggggatga ctgtaaccat taaaactaat
     1441 gcctgtcata atggaggggg attcagcgaa gttatttttc gttgactcag aatagctcag
     1501 tgaaaatagc aggcggagat tcataaatgt taaatacatc tcaattcagt cagttgttgc
     1561 ctgtctgata atagatgtgt tagaaaattt ctgcatggtg aatccccctg tgcggagggg
     1621 cgactggtga acggtatgat ctctttgatg atcgtaagcg agaatacgcg ggtttggtgg
     1681 caccaggccg aactcaccgg gaggcacccg gcaccatgca gtattcagag attaggcata
     1741 tatccaggct cctcatcgca ggagcctttt tacatgcaaa aaaaaacgct cctgggaaga
     1801 gcggctggca agaagaaaca atatgaacaa ttaattaacg atgggaataa taccttagag
     1861 taatcacctt gcgcaactgt aagggcatat ttcatctttg cgggctgttt ttctgtgtgg
     1921 cttctgtgtt tccggagggc agcctgtacc ttttctgact cagaacatta tcccggccgg
     1981 gaggattcat ggcatttaaa cac
//
LOCUS       DAZGHF010000558         1115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.guided.387, whole genome shotgun sequence.
ACCESSION   DAZGHF010000558 DAZGHF010000000
VERSION     DAZGHF010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.guided.387"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHK3A_005494"
     CDS             132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHK3A_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO8743396.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
BASE COUNT          297 a          237 c          268 g          313 t
ORIGIN      
        1 aagcaatcgg tcactggttc gaatccagta caacgcgcca tacttatttt ttctggctcg
       61 cttttgcggg ccttttttat atctgcgccg ggtctggtgc tgattacttc agccaaaagg
      121 aatacctgta tatgaagtgt atattgttaa aatgggtact gtgcctgtta ctgggctttt
      181 cttcggtatc ctattcccgg gaatttacga tagacttttc gacccaacaa agttatgtct
      241 cttcgttaaa tagtatacgg acagaaatat cgaccccact tgaacatata tctcagggga
      301 ccacatcggt gtctgttatt aaccacaccc caccgggcag ttattttgct gtggatatac
      361 gagggcttga tgtctatcag gcgcgttttg accatcttcg tctgattatt gagcaaaata
      421 atttatatgt ggccggattc gttaatacgg caacaaatac tttctacagg ttttcagatt
      481 ttgcacatat atcagtgccc ggtgtgacaa ctgtttccat gacaacggac agcagttata
      541 ccactctgca gcgtgtcgca gcgctggaac gttccggaat gcaaatcagt cgtcactcac
      601 tggtttcatc atatctggcg ttaatggagt ttagtggaaa tgccatgacc agagatgcat
      661 ccagagcagt tctgcgtttt gtcactgtca cagcagaagc cttacggttc aggcaaatac
      721 agagagaatt tcgtctggca ctgtctgaaa ctgctcctgt ttatacgatg acaccggaag
      781 aagtggacct cacactgaac tgggggagaa tcagcaatgt gcttccggag tttcggggag
      841 agggtggtgt cagagtgggg cgaatatcct ttaataatat atcagcgata ctgggcacag
      901 tggcggttat actgaattgc catcatcagg gggcgcgttc cgttcgcgcc gtgaatgaag
      961 agatacaacc agaatgtcag ataactggcg acaggccagt tataaggata aacaatacgt
     1021 tatgggaaag taataccgca gctgcttttc tgaatcgcag ggctcactct ttaaatacat
     1081 ccggagaata acaggagtta aatatgaaga agata
//
LOCUS       DAZGHF010000559          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2008W48534
            SAMN43267324-rid23072063.guided.513, whole genome shotgun sequence.
ACCESSION   DAZGHF010000559 DAZGHF010000000
VERSION     DAZGHF010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267324
            Sequence Read Archive: SRR30307828
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351145.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351145.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:21:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 88
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 122 of 169
            Pseudo Genes (internal stop)      :: 46 of 169
            Pseudo Genes (multiple problems)  :: 51 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267324-rid23072063.guided.513"
                     /serotype="O146:H28"
                     /isolate="2008W48534"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            29..292
                     /gene="stxB2b"
                     /locus_tag="ACHK3A_005495"
     CDS             29..292
                     /gene="stxB2b"
                     /locus_tag="ACHK3A_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO8743397.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          123 a           63 c           94 g          110 t
ORIGIN      
        1 tacatccgga gaataacagg agttaaatat gaagaagata tttgtagcgg ctttatttgc
       61 ttttgtttct gttaatgcaa tggcagctga ttgtgcaaaa ggtaaaattg agttctctaa
      121 gtataatgag aatgatacat tcacagtaaa agtggccggg aaagagtact ggactaaccg
      181 ctggaatctg caaccgctac tgcaaagcgc acagttaaca ggaatgacgg taacaatcaa
      241 atcaaatacc tgtgcgtcag gttcaggatt tgctgaagtg cagtttaatt aatatcagaa
      301 gcattgctgg tttcgtggtg tgcagcaatg tagttacagt gtaatcaatg tcacaattca
      361 gtcagttgaa ggttgtctgc ccgactgaga
//
